LOCUS       DADMGC010000001       111748 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.001, whole genome shotgun sequence.
ACCESSION   DADMGC010000001 DADMGC010000000
VERSION     DADMGC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 111748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 111748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..111748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.001"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            606..2891
                     /gene="nrdA"
                     /locus_tag="JNZ73_000001"
     CDS             606..2891
                     /gene="nrdA"
                     /locus_tag="JNZ73_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAY8730429.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3125..4255
                     /gene="nrdB"
                     /locus_tag="JNZ73_000002"
     CDS             3125..4255
                     /gene="nrdB"
                     /locus_tag="JNZ73_000002"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HAY8730430.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4255..4509
                     /gene="yfaE"
                     /locus_tag="JNZ73_000003"
     CDS             4255..4509
                     /gene="yfaE"
                     /locus_tag="JNZ73_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HAY8730431.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4563..5213)
                     /gene="inaA"
                     /locus_tag="JNZ73_000004"
     CDS             complement(4563..5213)
                     /gene="inaA"
                     /locus_tag="JNZ73_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HAY8730432.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSRVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5377..5619
                     /locus_tag="JNZ73_000005"
                     /pseudo
     CDS             5377..5619
                     /locus_tag="JNZ73_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5661..6737)
                     /gene="glpQ"
                     /locus_tag="JNZ73_000006"
     CDS             complement(5661..6737)
                     /gene="glpQ"
                     /locus_tag="JNZ73_000006"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY8730433.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6742..8100)
                     /gene="glpT"
                     /locus_tag="JNZ73_000007"
     CDS             complement(6742..8100)
                     /gene="glpT"
                     /locus_tag="JNZ73_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HAY8730434.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8373..10001
                     /gene="glpA"
                     /locus_tag="JNZ73_000008"
     CDS             8373..10001
                     /gene="glpA"
                     /locus_tag="JNZ73_000008"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HAY8730435.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIAHHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9991..11250
                     /gene="glpB"
                     /locus_tag="JNZ73_000009"
     CDS             9991..11250
                     /gene="glpB"
                     /locus_tag="JNZ73_000009"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HAY8730436.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11247..12437
                     /gene="glpC"
                     /locus_tag="JNZ73_000010"
     CDS             11247..12437
                     /gene="glpC"
                     /locus_tag="JNZ73_000010"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HAY8730437.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12630..13529
                     /locus_tag="JNZ73_000011"
     CDS             12630..13529
                     /locus_tag="JNZ73_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY8730438.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDESDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGFEREIVLAATQLTDADIPNCY"
     gene            13548..13769
                     /locus_tag="JNZ73_000012"
     CDS             13548..13769
                     /locus_tag="JNZ73_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8730439.1"
                     /translation="MTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQEGMHEQAIKIA
                     LRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13810..14280)
                     /locus_tag="JNZ73_000013"
     CDS             complement(13810..14280)
                     /locus_tag="JNZ73_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY8730440.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14335..15537)
                     /locus_tag="JNZ73_000014"
     CDS             complement(14335..15537)
                     /locus_tag="JNZ73_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HAY8730441.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSSRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15624..16166)
                     /locus_tag="JNZ73_000015"
     CDS             complement(15624..16166)
                     /locus_tag="JNZ73_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8730442.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16445..16870
                     /gene="nudI"
                     /locus_tag="JNZ73_000016"
     CDS             16445..16870
                     /gene="nudI"
                     /locus_tag="JNZ73_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HAY8730443.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16909..17511)
                     /locus_tag="JNZ73_000017"
     CDS             complement(16909..17511)
                     /locus_tag="JNZ73_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAY8730444.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNMVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17819..18958
                     /gene="arnB"
                     /locus_tag="JNZ73_000018"
     CDS             17819..18958
                     /gene="arnB"
                     /locus_tag="JNZ73_000018"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HAY8730445.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDTIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18962..19930
                     /gene="arnC"
                     /locus_tag="JNZ73_000019"
     CDS             18962..19930
                     /gene="arnC"
                     /locus_tag="JNZ73_000019"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HAY8730446.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19930..21912
                     /gene="arnA"
                     /locus_tag="JNZ73_000020"
     CDS             19930..21912
                     /gene="arnA"
                     /locus_tag="JNZ73_000020"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HAY8730447.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDFVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            21909..22799
                     /locus_tag="JNZ73_000021"
     CDS             21909..22799
                     /locus_tag="JNZ73_000021"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HAY8730448.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22799..24451
                     /gene="arnT"
                     /locus_tag="JNZ73_000022"
     CDS             22799..24451
                     /gene="arnT"
                     /locus_tag="JNZ73_000022"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HAY8730449.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDLWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24448..24783
                     /gene="arnE"
                     /locus_tag="JNZ73_000023"
     CDS             24448..24783
                     /gene="arnE"
                     /locus_tag="JNZ73_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HAY8730450.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24783..25169
                     /gene="arnF"
                     /locus_tag="JNZ73_000024"
     CDS             24783..25169
                     /gene="arnF"
                     /locus_tag="JNZ73_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HAY8730451.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25163..25435)
                     /gene="pmrD"
                     /locus_tag="JNZ73_000025"
     CDS             complement(25163..25435)
                     /gene="pmrD"
                     /locus_tag="JNZ73_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HAY8730452.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25540..26895)
                     /gene="menE"
                     /locus_tag="JNZ73_000026"
     CDS             complement(25540..26895)
                     /gene="menE"
                     /locus_tag="JNZ73_000026"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HAY8730453.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADVLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26892..27854)
                     /gene="menC"
                     /locus_tag="JNZ73_000027"
     CDS             complement(26892..27854)
                     /gene="menC"
                     /locus_tag="JNZ73_000027"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HAY8730454.1"
                     /translation="MRSAQVYRWQIPMDAGVLLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(27854..28711)
                     /gene="menB"
                     /locus_tag="JNZ73_000028"
     CDS             complement(27854..28711)
                     /gene="menB"
                     /locus_tag="JNZ73_000028"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HAY8730455.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28726..29484)
                     /gene="menH"
                     /locus_tag="JNZ73_000029"
     CDS             complement(28726..29484)
                     /gene="menH"
                     /locus_tag="JNZ73_000029"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HAY8730456.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIIEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29481..31151)
                     /gene="menD"
                     /locus_tag="JNZ73_000030"
     CDS             complement(29481..31151)
                     /gene="menD"
                     /locus_tag="JNZ73_000030"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HAY8730457.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIGNIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31240..32535)
                     /gene="menF"
                     /locus_tag="JNZ73_000031"
     CDS             complement(31240..32535)
                     /gene="menF"
                     /locus_tag="JNZ73_000031"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HAY8730458.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32614..32919)
                     /gene="elaB"
                     /locus_tag="JNZ73_000032"
     CDS             complement(32614..32919)
                     /gene="elaB"
                     /locus_tag="JNZ73_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HAY8730459.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGEPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32974..33435)
                     /locus_tag="JNZ73_000033"
     CDS             complement(32974..33435)
                     /locus_tag="JNZ73_000033"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8730460.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33500..34417
                     /gene="rbn"
                     /locus_tag="JNZ73_000034"
     CDS             33500..34417
                     /gene="rbn"
                     /locus_tag="JNZ73_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HAY8730461.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TAQRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34605..35825
                     /gene="elaD"
                     /locus_tag="JNZ73_000035"
     CDS             34605..35825
                     /gene="elaD"
                     /locus_tag="JNZ73_000035"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HAY8730462.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTGPDNSDVLLFKKLFSPSKLMTIIGNEIPLISEKQSLSKVLLNDENNELSDGT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFPINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKENSLKRVI
                     NSFNSELMGKMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRESN
                     SDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEIK
                     HSQPKNRASGKRVS"
     gene            36368..36860
                     /locus_tag="JNZ73_000036"
                     /pseudo
     CDS             36368..36860
                     /locus_tag="JNZ73_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            36963..37466
                     /locus_tag="JNZ73_000037"
     CDS             36963..37466
                     /locus_tag="JNZ73_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HAY8730463.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLDEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(37533..38990)
                     /gene="nuoN"
                     /locus_tag="JNZ73_000038"
     CDS             complement(37533..38990)
                     /gene="nuoN"
                     /locus_tag="JNZ73_000038"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HAY8730464.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFDYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(38997..40526)
                     /gene="nuoM"
                     /locus_tag="JNZ73_000039"
     CDS             complement(38997..40526)
                     /gene="nuoM"
                     /locus_tag="JNZ73_000039"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HAY8730465.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITVATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(40690..42531)
                     /gene="nuoL"
                     /locus_tag="JNZ73_000040"
     CDS             complement(40690..42531)
                     /gene="nuoL"
                     /locus_tag="JNZ73_000040"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HAY8730466.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAAQV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(42528..42830)
                     /gene="nuoK"
                     /locus_tag="JNZ73_000041"
     CDS             complement(42528..42830)
                     /gene="nuoK"
                     /locus_tag="JNZ73_000041"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HAY8730467.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(42827..43381)
                     /gene="nuoJ"
                     /locus_tag="JNZ73_000042"
     CDS             complement(42827..43381)
                     /gene="nuoJ"
                     /locus_tag="JNZ73_000042"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAY8730468.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(43393..43935)
                     /gene="nuoI"
                     /locus_tag="JNZ73_000043"
     CDS             complement(43393..43935)
                     /gene="nuoI"
                     /locus_tag="JNZ73_000043"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HAY8730469.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(43950..44927)
                     /gene="nuoH"
                     /locus_tag="JNZ73_000044"
     CDS             complement(43950..44927)
                     /gene="nuoH"
                     /locus_tag="JNZ73_000044"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HAY8730470.1"
                     /translation="MSWISPELIEILLTILKAVGILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(44924..47650)
                     /gene="nuoG"
                     /locus_tag="JNZ73_000045"
     CDS             complement(44924..47650)
                     /gene="nuoG"
                     /locus_tag="JNZ73_000045"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HAY8730471.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(47703..49040)
                     /gene="nuoF"
                     /locus_tag="JNZ73_000046"
     CDS             complement(47703..49040)
                     /gene="nuoF"
                     /locus_tag="JNZ73_000046"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAY8730472.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(49037..49537)
                     /gene="nuoE"
                     /locus_tag="JNZ73_000047"
     CDS             complement(49037..49537)
                     /gene="nuoE"
                     /locus_tag="JNZ73_000047"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HAY8730473.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(49540..51342)
                     /gene="nuoC"
                     /locus_tag="JNZ73_000048"
     CDS             complement(49540..51342)
                     /gene="nuoC"
                     /locus_tag="JNZ73_000048"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HAY8730474.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRTQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(51436..52098)
                     /locus_tag="JNZ73_000049"
     CDS             complement(51436..52098)
                     /locus_tag="JNZ73_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HAY8730475.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(52114..52557)
                     /gene="nuoA"
                     /locus_tag="JNZ73_000050"
     CDS             complement(52114..52557)
                     /gene="nuoA"
                     /locus_tag="JNZ73_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HAY8730476.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRECMNPETNSIANRQR"
     gene            complement(53188..54126)
                     /gene="lrhA"
                     /locus_tag="JNZ73_000051"
     CDS             complement(53188..54126)
                     /gene="lrhA"
                     /locus_tag="JNZ73_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HAY8730477.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            55105..56322
                     /locus_tag="JNZ73_000052"
     CDS             55105..56322
                     /locus_tag="JNZ73_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAY8730478.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            56406..57005
                     /gene="yfbR"
                     /locus_tag="JNZ73_000053"
     CDS             56406..57005
                     /gene="yfbR"
                     /locus_tag="JNZ73_000053"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY8730479.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(57064..58896)
                     /locus_tag="JNZ73_000054"
     CDS             complement(57064..58896)
                     /locus_tag="JNZ73_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAY8730480.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(58983..59633)
                     /gene="hxpA"
                     /locus_tag="JNZ73_000055"
     CDS             complement(58983..59633)
                     /gene="hxpA"
                     /locus_tag="JNZ73_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HAY8730481.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALSGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(59644..60138)
                     /locus_tag="JNZ73_000056"
     CDS             complement(59644..60138)
                     /locus_tag="JNZ73_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAY8730482.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(60221..60676)
                     /locus_tag="JNZ73_000057"
     CDS             complement(60221..60676)
                     /locus_tag="JNZ73_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HAY8730483.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            61014..62216
                     /gene="ackA"
                     /locus_tag="JNZ73_000058"
     CDS             61014..62216
                     /gene="ackA"
                     /locus_tag="JNZ73_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAY8730484.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            62291..64435
                     /gene="pta"
                     /locus_tag="JNZ73_000059"
     CDS             62291..64435
                     /gene="pta"
                     /locus_tag="JNZ73_000059"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY8730485.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            64625..66145
                     /gene="yfcC"
                     /locus_tag="JNZ73_000060"
     CDS             64625..66145
                     /gene="yfcC"
                     /locus_tag="JNZ73_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAY8730486.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(66178..66720)
                     /gene="yfcD"
                     /locus_tag="JNZ73_000061"
     CDS             complement(66178..66720)
                     /gene="yfcD"
                     /locus_tag="JNZ73_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HAY8730487.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(66778..67329)
                     /gene="yfcE"
                     /locus_tag="JNZ73_000062"
     CDS             complement(66778..67329)
                     /gene="yfcE"
                     /locus_tag="JNZ73_000062"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HAY8730488.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(67385..68029)
                     /gene="yfcF"
                     /locus_tag="JNZ73_000063"
     CDS             complement(67385..68029)
                     /gene="yfcF"
                     /locus_tag="JNZ73_000063"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HAY8730489.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            68165..68812
                     /gene="yfcG"
                     /locus_tag="JNZ73_000064"
     CDS             68165..68812
                     /gene="yfcG"
                     /locus_tag="JNZ73_000064"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HAY8730490.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            68869..69231
                     /gene="folX"
                     /locus_tag="JNZ73_000065"
     CDS             68869..69231
                     /gene="folX"
                     /locus_tag="JNZ73_000065"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HAY8730491.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            69252..70145
                     /locus_tag="JNZ73_000066"
     CDS             69252..70145
                     /locus_tag="JNZ73_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HAY8730492.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSHWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(70193..71083)
                     /locus_tag="JNZ73_000067"
     CDS             complement(70193..71083)
                     /locus_tag="JNZ73_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY8730493.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIA
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(71280..72053)
                     /gene="hisP"
                     /locus_tag="JNZ73_000068"
     CDS             complement(71280..72053)
                     /gene="hisP"
                     /locus_tag="JNZ73_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HAY8730494.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEACERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(72061..72777)
                     /locus_tag="JNZ73_000069"
     CDS             complement(72061..72777)
                     /locus_tag="JNZ73_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8730495.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(72774..73460)
                     /locus_tag="JNZ73_000070"
     CDS             complement(72774..73460)
                     /locus_tag="JNZ73_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708190.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY8730496.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     LGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(73550..74332)
                     /gene="hisJ"
                     /locus_tag="JNZ73_000071"
     CDS             complement(73550..74332)
                     /gene="hisJ"
                     /locus_tag="JNZ73_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HAY8730497.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(74553..75335)
                     /gene="argT"
                     /locus_tag="JNZ73_000072"
     CDS             complement(74553..75335)
                     /gene="argT"
                     /locus_tag="JNZ73_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HAY8730498.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALLPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(75601..76170)
                     /locus_tag="JNZ73_000073"
     CDS             complement(75601..76170)
                     /locus_tag="JNZ73_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY8730499.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(76265..77782)
                     /gene="purF"
                     /locus_tag="JNZ73_000074"
     CDS             complement(76265..77782)
                     /gene="purF"
                     /locus_tag="JNZ73_000074"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAY8730500.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(77819..78307)
                     /gene="cvpA"
                     /locus_tag="JNZ73_000075"
     CDS             complement(77819..78307)
                     /gene="cvpA"
                     /locus_tag="JNZ73_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HAY8730501.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(78566..79228)
                     /gene="dedD"
                     /locus_tag="JNZ73_000076"
     CDS             complement(78566..79228)
                     /gene="dedD"
                     /locus_tag="JNZ73_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HAY8730502.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(79218..80486)
                     /gene="folC"
                     /locus_tag="JNZ73_000077"
     CDS             complement(79218..80486)
                     /gene="folC"
                     /locus_tag="JNZ73_000077"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAY8730503.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIELGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGRELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADAAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(80556..81470)
                     /gene="accD"
                     /locus_tag="JNZ73_000078"
     CDS             complement(80556..81470)
                     /gene="accD"
                     /locus_tag="JNZ73_000078"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HAY8730504.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(81626..82285)
                     /locus_tag="JNZ73_000079"
     CDS             complement(81626..82285)
                     /locus_tag="JNZ73_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8730505.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(82368..83180)
                     /gene="truA"
                     /locus_tag="JNZ73_000080"
     CDS             complement(82368..83180)
                     /gene="truA"
                     /locus_tag="JNZ73_000080"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAY8730506.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(83180..84193)
                     /locus_tag="JNZ73_000081"
     CDS             complement(83180..84193)
                     /locus_tag="JNZ73_000081"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY8730507.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQLWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(84259..85395)
                     /gene="pdxB"
                     /locus_tag="JNZ73_000082"
     CDS             complement(84259..85395)
                     /gene="pdxB"
                     /locus_tag="JNZ73_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HAY8730508.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            85494..86489
                     /locus_tag="JNZ73_000083"
     CDS             85494..86489
                     /locus_tag="JNZ73_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HAY8730509.1"
                     /translation="MIQPISGPPLGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(86486..87664)
                     /locus_tag="JNZ73_000084"
     CDS             complement(86486..87664)
                     /locus_tag="JNZ73_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8730510.1"
                     /translation="MTAVSQTETRSSASFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLCFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(87939..89159)
                     /gene="fabB"
                     /locus_tag="JNZ73_000085"
     CDS             complement(87939..89159)
                     /gene="fabB"
                     /locus_tag="JNZ73_000085"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HAY8730511.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            89318..91324
                     /gene="mnmC"
                     /locus_tag="JNZ73_000086"
     CDS             89318..91324
                     /gene="mnmC"
                     /locus_tag="JNZ73_000086"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HAY8730512.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVLVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAR"
     gene            complement(91445..91723)
                     /locus_tag="JNZ73_000087"
     CDS             complement(91445..91723)
                     /locus_tag="JNZ73_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HAY8730513.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(91757..92305)
                     /locus_tag="JNZ73_000088"
     CDS             complement(91757..92305)
                     /locus_tag="JNZ73_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HAY8730514.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(92305..93114)
                     /locus_tag="JNZ73_000089"
     CDS             complement(92305..93114)
                     /locus_tag="JNZ73_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAY8730515.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(93114..93938)
                     /gene="mepA"
                     /locus_tag="JNZ73_000090"
     CDS             complement(93114..93938)
                     /gene="mepA"
                     /locus_tag="JNZ73_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HAY8730516.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(93942..95027)
                     /gene="aroC"
                     /locus_tag="JNZ73_000091"
     CDS             complement(93942..95027)
                     /gene="aroC"
                     /locus_tag="JNZ73_000091"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAY8730517.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKEGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(95062..95994)
                     /gene="prmB"
                     /locus_tag="JNZ73_000092"
     CDS             complement(95062..95994)
                     /gene="prmB"
                     /locus_tag="JNZ73_000092"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY8730518.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            96160..96711
                     /gene="smrB"
                     /locus_tag="JNZ73_000093"
     CDS             96160..96711
                     /gene="smrB"
                     /locus_tag="JNZ73_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HAY8730519.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(96815..97705)
                     /locus_tag="JNZ73_000094"
     CDS             complement(96815..97705)
                     /locus_tag="JNZ73_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HAY8730520.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(97770..98216)
                     /locus_tag="JNZ73_000095"
     CDS             complement(97770..98216)
                     /locus_tag="JNZ73_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8730521.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNT"
     gene            complement(98213..98683)
                     /locus_tag="JNZ73_000096"
     CDS             complement(98213..98683)
                     /locus_tag="JNZ73_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8730522.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(98680..99228)
                     /locus_tag="JNZ73_000097"
     CDS             complement(98680..99228)
                     /locus_tag="JNZ73_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8730523.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(99233..99955)
                     /locus_tag="JNZ73_000098"
     CDS             complement(99233..99955)
                     /locus_tag="JNZ73_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY8730524.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPYQYKV
                     TLTRNGQEFTVNNPTSYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSC"
     gene            complement(99975..102616)
                     /gene="yfcU"
                     /locus_tag="JNZ73_000099"
                     /pseudo
     CDS             complement(99975..102616)
                     /gene="yfcU"
                     /locus_tag="JNZ73_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(102698..103261)
                     /locus_tag="JNZ73_000100"
     CDS             complement(102698..103261)
                     /locus_tag="JNZ73_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY8730525.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(103945..104430)
                     /gene="sixA"
                     /locus_tag="JNZ73_000101"
     CDS             complement(103945..104430)
                     /gene="sixA"
                     /locus_tag="JNZ73_000101"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HAY8730526.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(104633..106777)
                     /gene="fadJ"
                     /locus_tag="JNZ73_000102"
     CDS             complement(104633..106777)
                     /gene="fadJ"
                     /locus_tag="JNZ73_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HAY8730527.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(106777..108087)
                     /gene="fadI"
                     /locus_tag="JNZ73_000103"
     CDS             complement(106777..108087)
                     /gene="fadI"
                     /locus_tag="JNZ73_000103"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HAY8730528.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(108267..108551)
                     /locus_tag="JNZ73_000104"
     CDS             complement(108267..108551)
                     /locus_tag="JNZ73_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HAY8730529.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            108923..110263
                     /gene="fadL"
                     /locus_tag="JNZ73_000105"
     CDS             108923..110263
                     /gene="fadL"
                     /locus_tag="JNZ73_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HAY8730530.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLQADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            110629..111687
                     /locus_tag="JNZ73_000106"
     CDS             110629..111687
                     /locus_tag="JNZ73_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8730531.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNCQAK
                     YQNEINQLSALGVNFDDI"
BASE COUNT        28004 a        29875 c        27470 g        26399 t
ORIGIN      
        1 tgcaatggct attttgttat tatttgatga ataatatcag tgcgtcataa ttcaagttaa
       61 taaccttaag ggataccagt tatatttaaa ctaaattaaa gtaatgaata attttcttat
      121 aatataaggt aaattaacaa aatggcttcg catttaacaa taaccgaata gaaaacaacc
      181 atttcgacat caacaatcgc ttacattcgc ttatatattg accacaactg atacatcaga
      241 ttatgtgatg actcgtgctt agatcaattt ttgcaatcat tagcaaaaag attaataaac
      301 catctatatc aatttatcca acctattctg ccgttcaaga aatcgccgaa cagttatttt
      361 taacaaattt ttctcttccc attgactttc ccggacacct tgtctgacct aaggtgcgcg
      421 aaagccactt tttccttcct gagttatcca caaagttatg cacttgcaag agggtcattt
      481 tcacactatc ttgcagtgaa tcccaaacat accccctata tatagtgttc taagcagctt
      541 cccttactac aggtagtctg catgaaacta ttgcggaaag aattccaaaa acaggtacga
      601 catacatgaa tcagaatctg ctggtgacaa agcgcgacgg tagcacagag cgcatcaatc
      661 tcgacaaaat ccatcgcgtt ctggattggg cggcagaagg actgcataac gtttcgattt
      721 cccaggtcga gctgcgctcc cacattcagt tttatgacgg tatcaagacc tctgacatcc
      781 acgaaactat tatcaaggct gccgcagacc tgatctcccg tgatgcgccg gattatcagt
      841 atctcgccgc gcgcctggcg atcttccacc tgcgtaaaaa agcctacggc cagtttgagc
      901 cgcctgcgct gtacgaccac gtggtgaaaa tggtcgagat gggcaaatac gataatcatc
      961 tgctggaaga ctacacggaa gaagagttca agcagatgga cacgtttatc gatcacgacc
     1021 gtgatatgac cttctcttat gctgccgtta agcagctgga aggcaaatat ctggtacaga
     1081 accgcgtgac cggcgaaatc tacgaaagcg cccagttcct ttatattctt gttgccgcgt
     1141 gcctgttctc gaactacccg cgtgaaacgc gcctgcaata cgtgaagcgt ttttacgacg
     1201 cggtttccac atttaaaatt tcgctgccga cgccaatcat gtccggcgtg cgtaccccga
     1261 ctcgtcagtt cagctcctgc gtactgatcg agtgcggtga cagcctggat tccatcaacg
     1321 ccacctccag cgcgattgtg aaatatgttt cccagcgtgc cgggatcggc atcaacgccg
     1381 gacgtattcg tgcgctgggc agcccgattc gcggtggcga agcgttccat accggctgta
     1441 tcccgttcta caaacatttc cagacagcgg tgaaatcctg ctctcagggc ggtgtgcgcg
     1501 gcggtgcggc aacgctgttc tacccgatgt ggcatctgga agtggaaagc ctgctggtgt
     1561 tgaaaaacaa ccgtggggtt gaaggcaacc gcgtgcgtca tatggactac ggggtacaaa
     1621 tcaacaaact gatgtatacc cgcctgctga aaggtgaaga tatcaccctg ttcagcccgt
     1681 ccgacgtacc ggggctgtac gacgcgttct tcgccgatca ggaagagttt gaacgcctgt
     1741 ataccaaata tgagaaagac gacagcatcc gcaagcagcg tgtgaaagcc gttgagctgt
     1801 tctcgctgat gatgcaggaa cgtgcgtcta ccggtcgtat ctatattcag aacgttgacc
     1861 actgtaatac ccatagtccg tttgatccga ccatcgcgcc agttcgccag tctaacctgt
     1921 gcctggaaat tgccctgccg accaaaccgc tgaacgacgt caacgacgag aacggtgaaa
     1981 tcgcgctgtg tacgctgtct gctttcaacc tgggcgcaat taatagcctg gatgaactgg
     2041 aagagctggc gattctggcg gttcgtgcac ttgacgcgct actggattat caggattacc
     2101 cgatcccggc cgccaaacgt ggagcgatgg ggcgtcgtac gctgggtatt ggtgtgatta
     2161 acttcgctta ctatctggcg aagcacggta aacgctactc cgacggcagc gccaacaacc
     2221 tgacgcataa aaccttcgaa gcgattcagt attacctgct gaaagcgtct aatgagctgg
     2281 ctaaagagca aggcgcgtgc ccgtggttta acgaaaccac ttacgcgaaa ggtatcctgc
     2341 cgatcgatac ctataagaaa gatctggatg ccatcgctaa tgagccgctg cattacgact
     2401 gggaagcatt gcgtgaatca atcaaaacgc acggcctgcg taactccacg ctttctgccc
     2461 tgatgccgtc cgagacctct tcgcagatct ctaacgccac taacggtatt gaaccgccgc
     2521 gcggttacgt cagcatcaaa gcgtcgaaag acggtatttt gcgccaggtg gtgccggact
     2581 acgagcacct gcacgacgcc tatgagctgc tgtgggaaat gccgggtaac gatggctacc
     2641 tgcaactggt aggtatcatg cagaaattta tcgatcagtc gatctctgct aacaccaact
     2701 acgatccgtc acgtttcccg tcaggaaaag tgccgatgca gcagttgctg aaagacctgc
     2761 tcaccgccta caaattcggg gtcaaaacgc tgtattatca gaacacccgt gacggcgctg
     2821 aagacgcgca ggacgatctg gtaccgtcaa tccaggacga tggctgcgaa agcggcgcat
     2881 gtaagatctg atattgagat gccggatgcg gcgtaaacgc cttatccggc ctacggctcg
     2941 gtttgtaggc ctgataagac gcgccagcgt cgcatcaggc tccgggtgcc ggatgcagcg
     3001 tgaacgcctt atccggccta cggctcggat ttgtaggcct gataagacgc gccagcgtcg
     3061 catcaggcac aggatgcggc gtaaaatgcc ttatccggca ttaaactccc aacaggacac
     3121 actcatggca tataccacct tttcacagac gaaaaatgat cagctcaaag aaccgatgtt
     3181 ctttggtcag ccggtcaacg tggctcgcta cgatcagcaa aaatatgaca tcttcgaaaa
     3241 gctgatcgaa aagcagctct ctttcttctg gcgtccggaa gaagttgacg tctcccgcga
     3301 ccgtatagat taccaggcgc tgccggagca cgaaaaacac atctttatca gcaacctgaa
     3361 atatcagacg ctgctggatt ccattcaggg tcgtagcccg aacgtggcgc tattgccgct
     3421 tatttctatt ccggaactgg aaacctgggt cgaaacctgg gcgttctcag aaacgattca
     3481 ttcccgttcc tatactcata tcattcgtaa tatcgttaac gatccgtctg ttgtgtttga
     3541 cgatatcgtc accaacgagc agatccagaa acgtgcggaa gggatctcca gctattacga
     3601 tgaactgatc gaaatgacca gctactggca ccttctgggc gaaggcaccc acaccgttaa
     3661 cggtaaaacc gtaaccgtta gcctgcgcga gctgaagaaa aaactgtatc tctgcctgat
     3721 gagcgttaac gcgctggaag cgatccgctt ctacgtcagc tttgcttgtt cttttgcctt
     3781 tgcagaacgc gaactgatgg aaggtaacgc caagattatt cgcctgattg cccgcgatga
     3841 agccctgcac ctgaccggta ctcagcatat gctgaatctg ctgcgcagcg gcgcggatga
     3901 tccggagatg gcggaaattg ccgaagaatg taagcaggag tgctatgacc tgtttgtcca
     3961 ggcggcacag caagagaaag actgggcgga ttatctgttc cgtgacggtt cgatgattgg
     4021 tctgaataaa gatattctct gccagtacgt tgaatacatc accaatatcc gcatgcaggc
     4081 agtcggtttg gatctgccgt tccagacgcg ctccaacccg atcccgtgga tcaacacctg
     4141 gctggtgtct gataacgtgc aggttgctcc gcaggaagtg gaagtcagtt cttatctggt
     4201 cgggcagatt gacgcggaag tggacaccga cgatttgagt aacttccagc tctgatggcc
     4261 cgcgttaccc tgcgcatcac tggcacacaa ctgctgtgcc aggatgaaca cccttccctt
     4321 ctggcggcgc tggaatccca caatgtggcg gttgagtacc agtgccgcga aggttactgc
     4381 ggctcctgtc gcacccgact ggtcgcaggt caggtggact ggattgccga accgttagcc
     4441 tttattcagc cgggggaaat tttgccctgt tgttgccggg caaaaggcga tattgaaatc
     4501 gagttgtgat cgggtactag cgccagatac gacgcttacg cgtattatct ggcctacaac
     4561 gattacatgg cgtagtaata cgctttcacc tgctcccagt ccgctttagg gatcggctcc
     4621 agatattttt ccagctggcg gaagtcatga ttaatcgctt tatcgcgacg caagcggcga
     4681 cgacttttct ccagatcaag aaaaccagct tctgccttac cttctgtttt cacatagata
     4741 tggcgaacat agcaacagcc atgctgacga ttaacgctat gcattttctt aaacgccagt
     4801 gccactgctt tcaacatggc ttgccgtact tcgtcagaat aaggcgatac tgcatgccgg
     4861 gcataccagt cagcaatgct gatgaacccc gccatatctt cagtcaccag caacgcgcgc
     4921 cattcaccct caattttcac cgcttcacca aaaacgatct tcggcacgat gacaccggcc
     4981 cgttccagtt ctttaattac cgcaacctca cggacaatcg ttggtcggcc gaacggataa
     5041 cgtactgaat gaaacagatg atgcgtcatg cgctttacat acagcttttt gccgttgcgc
     5101 tcgacgcatt gcaccctgct cataccatta cggcgatagt taggctcttc aacccagtcg
     5161 ccctctgttg cccaccagtg attaaattcg tcgtactttg ctgaaactgc cataccctat
     5221 cgcctgtcat ttttattaac gacaatgact ataggtggtt acctgagcaa aatcttaatg
     5281 aaacgtgtcg tattccgcta caggtgtgac accgcttttg cccttcattt gcagcgcata
     5341 gattccacca gccactacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag
     5401 tcggatacag cgatgaattt tattcgacag ggattaggca tcgccttaca accagagtta
     5461 acgctgaaaa gcattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga
     5521 cagatttcgt tgctgactaa agaaaagccg gtagaaggca gtccactgtt tttactacaa
     5581 atgtgcatgg aacaattagt ggcgattgga aaaatttgat atgagacagg atggcgcatg
     5641 aacgccatcc tgcagtaaca ttactcttta ttaagaaatt ttactgcctt atcagggaaa
     5701 tcagtaaaca gcccatttac acccgctttg ttatacagag catcatataa ctgattcaca
     5761 tcaggagtgt attcaggcag tttatctgac cgcacggtat aaggatgcac taccagttta
     5821 ttctgctgag catcttgcac catgccagtg agtttgatat tacccggctg cgatgtctcc
     5881 tcaatcaaca tatggtaatc cggaccaata ccatctgcat attccgccac ctgtttcatg
     5941 gcacccggct taaacatcca gtcgtagttg taattaaccc agcttccatc cggctgtttc
     6001 tgctgcgttt cattccagtc ggtataggca atcagctgta ccagattgag ctccatgccc
     6061 attttgggtt ccagctcatt cttaatacgc ttcagctcat cagcatcaaa acattgcaaa
     6121 taaactttat cgtctttacc ggtgtaacca tatttcttca gcacttccag cgtttttgcc
     6181 gcaatatcct tcccttcctg atgatggaac cacggcgctt tgatttctgg atagataccg
     6241 atatttttcc cggtagagtg atttaacccc tgaacaaatt caatctcttc ttcaaaggtg
     6301 tgcacccgga agtcggactt acccattggg aaacgccccg gataagtctg tactttttta
     6361 ccgttttcaa tatcgaaacc ttcggtaaat ttcagcgact taatttcatc cagcgtgaaa
     6421 tctatcgcgt agtaacgacc gtctttgcgc gcccgatccg ggaaacgatc ggcaacatca
     6481 gtaacacgat cgagataatg gtcatgcaga acaaccagat ggtcgtcttt ggtcatcacc
     6541 aaatcctgct ccagataatc cgctccctgc gcatacgcca tcgcttttgc tggcagcgta
     6601 tgctccggca aatatccact ggcaccgcga tgggcgatga ctattttttc gttgctgtcc
     6661 gccgccattg cactgcttcc catgactata gtgctcatca tgatcgccat gctaaggttt
     6721 ttcagcttca atttcatgcc attagcctcc gttgcgtttt tgcagtaatt gttcatggcg
     6781 acgtttttcg ccaatcatca caacaatcaa caagataacc gccagaatgc tgccgccaat
     6841 cattaccata aagccgccat cccagccgaa gaagtccacg gtgtagccaa caatcgcgct
     6901 cgccgccacc gaaccgccca ggtaaccaaa cagcccggta aagcccgctg ccgtacctgc
     6961 cgcttttttc ggtgccagtt ccagcgcatg cagaccgatc agcatcacag gaccgtagat
     7021 caggaagccg ataacaatca tacaaatcat atcgacggtt gggttacctg ccgggttcat
     7081 ccagtaaacg atagtcgcga tggtcaccag tgtcataaag aagacgccgg ttgccccacg
     7141 gttaccacgg aagactttgt ccgacatcca gccgcacagc agggtgcccg gaatacccgc
     7201 atactcatag aagaagtagg cccaggagga tttatccagc gcgaaatgct taacctcttt
     7261 cagataagtc ggtgaccagt cgaggatgcc gtaacgcagc agataaacga acacgttggc
     7321 gatggcgatg taccacagca gtttgttcgg cagcacgtac tgcatgaaga tttgcttcgc
     7381 ggtcagctcc tgttccgctt tttcgttata gtcgtccgga taatcatttt tgtactcttc
     7441 gatcggcggc aagccacagg attgcggggt atcgcgcatc atcgcaaagg cgaataatgc
     7501 caccagaatg gcgcagaaag caggcatata gagcgccgca tgccagtcat tgaaccaggc
     7561 catccccagc aggaacagca gcggcggaat accaccaccg acgttgtgcg cacagttcca
     7621 cactgacaca atgccgccac gttctttctg cgaccaccag tgcaccatag tacgaccaca
     7681 cggcggccac cccatcccct ggaaccaacc gcagaggaac aacagtacaa acatcaccgc
     7741 aatgctcgac gtcgcccatg gcacaaagcc cataaacaac atcactgccg ccgccagaat
     7801 caaacctgcg ggcaggaaaa cgcgcggatt cgagcgatcc gataccgaac ccatgatgaa
     7861 tttcgaaaat ccataagcaa tcgagatccc cgaaagggca aaacctaaat caccgcgtga
     7921 gaatccctgc tcaaccagat aaggcatagc aagcgcaaag ttcttacgaa ccaaatagta
     7981 agccgcatag ccaaagaata tccccaggaa aatttgccag cgcaatcgac gataagtcgg
     8041 atcgatctcc gcggcaggta agcgcgcttt gtgtggcgct ggtttaaaaa tactcaacat
     8101 tgatagcctc cgtggcccgt ggtcttattt atgattaaca gcctgattca gtgagagaac
     8161 ctgccgtttc ttgagttgcc gcgatgttaa gaaaacattc ataaattaaa tgtgaattgc
     8221 cgcacacatt attaaataag atttacaaaa tgttcaaaat gacgcatgaa atcacgtttc
     8281 actttcgaat tatgatcgaa tatgcgcgaa atcaaacaat tcatgttttt actatggcta
     8341 aatggtaaaa aacgaacttc agagggataa caatgaaaac tcgcgactcg caatcaagtg
     8401 acgtgattat cattggcggc ggcgcaacgg gagccgggat tgcccgcgac tgtgccctgc
     8461 gcgggctgcg cgtgattttg gttgagcgcc acgacatcgc aaccggtgcc accgggcgta
     8521 accacggcct gctgcacagc ggtgcgcgct atgcagtaac cgatgcggaa tcggcccgcg
     8581 aatgcattag tgaaaaccag atcctgaaac gcattgcaca tcactgcgtt gaaccaacca
     8641 acggcctgtt tatcaccctg ccggaagatg acctctcatt ccaggccact tttattcgcg
     8701 cctgcgaaga agcagggatc agcgcagaag ctatagaccc gcagcaagcg cgcattatcg
     8761 aacctgccgt taacccggca ctgattggcg cggtgaaagt tccggatggc accgttgatc
     8821 catttcgtct gaccgcagca aacatgctgg atgccaaaga acacggtgcc gttatcctta
     8881 ccgctcatga agtcacgggg ctgattcgtg aaggcgcgac ggtgtgcggt gttcgtgtac
     8941 gtaaccatct caccggcgaa actcaggccc ttcatgcacc tgtcgtggtt aatgccgctg
     9001 ggatctgggg gcaacacatt gccgaatatg ccgatctgcg cattcgcatg ttcccggcga
     9061 aaggatcgct gctgatcatg gatcaccgca ttaaccagca tgtgatcaac cgctgccgta
     9121 aaccttccga cgccgatatt ctggtgcctg gcgataccat ttcgctgatt ggtaccacct
     9181 ctttacgtat tgattacaac gagattgacg ataatcgagt gacggcagaa gaggttgata
     9241 ttctgctgcg tgaaggggaa aaactggccc ccgtgatggc gaaaacgcgc attttgcggg
     9301 cctattctgg cgtgcgcccg ctggttgcca gcgatgacga cccgagcgga cgtaacgtca
     9361 gccgtggcat cgtgttgctc gaccatgctg aacgcgatgg tctggacgga tttatcacca
     9421 tcaccggtgg caaactgatg acctatcggc tgatggctga atgggctacc gacgcggtat
     9481 gccgcaaact gggcaacacg cgcccctgta cgactgccga tctggcactg cctggttcac
     9541 aagaacccgc tgaagttacc ttgcgtaaag tcatctccct gcctgccccg ctgcgcggtt
     9601 ctgcggttta tcgtcatggc gatcgcacgc ctgcctggct gagcgaaggc cgtctacacc
     9661 gtagcctggt atgtgagtgc gaagcggtaa ctgcgggtga agtgcagtac gcggtagaaa
     9721 atttaaacgt taatagcctg ctggatttac gccgtcgtac ccgtgtgggg atgggcacct
     9781 gccagggcga actctgcgcc tgccgcgctg ccggactgct gcaacggttt aacgtcacga
     9841 cgtccgcgca atctatcgag caactttcca ccttccttaa cgaacgctgg aaaggcgtgc
     9901 aacccatcgc ctggggagac gcactgcgcg aaagcgaatt tacccgctgg gtttatcagg
     9961 gactgtgtgg tctggagaag gagcagaaag atgcgctttg atactgtcat tatgggcggc
    10021 ggcctcgccg gattactctg tggcctgcaa ctgcaaaaac acggcctgcg ctgtgccatt
    10081 gtcacgcgtg gtcaaagcgc gttgcatttc tcatccggtt cgctggattt gctgagccat
    10141 ctgccagatg gtcaacccgt ggcagacatt cacagtggac tggaatcttt gcgtcagcag
    10201 gcacctgcac atccttactc tcttctcggg ccacaacgcg tgctcgatct cgcttgccag
    10261 gcgcaggcat taatcgctga aagcggtgcg caattgcagg gcagcgtaga actggctcac
    10321 cagcggatta cgccgctcgg cactttgcgt gcaacctggt taagttcgcc agaagttccc
    10381 gtctggccgc tacccgcgaa gaagatatgt gtagtgggga taagcgggct gatggatttc
    10441 caggcgcatc ttgcggcggc ttcgttgcgc gaactggatc tttcggttga aaccgcagaa
    10501 atagagctgc cggaactgga tgtcctgcgc aataacgcca ccgaatttcg cgcggtgaat
    10561 atcgcccgtt tccttgataa tgaagaaaac tggccgctgt tacttgatgc gcttattcct
    10621 gtcgccaata cctgcgaaat gatcctgatg cccgcctgct tcggcctagc cgatgacaaa
    10681 ctgtggcgtt ggttgaatga aaaactacct tgttcactga tgcttttgcc aacgctgccg
    10741 ccttccgtgc tgggcattcg tctgcaaaac cagttacagc gccagtttgt gcgccagggt
    10801 ggagtgtgga tgccgggcga tgaagtgaaa aaagtgacct gtaaaaatgg cgtagtgaat
    10861 gaaatctgga cccgcaatca cgccgatatt ccgctacgtc cacgtttcgc ggttctcgcc
    10921 agcggcagtt tctttagtgg cggactggta gcggaacgta acggcattcg cgagccgatt
    10981 ctcggccttg atgtgctaca aaccgccacg cggggtgaat ggtataaggg cgattttttt
    11041 gcgccgcaac cgtggcagca gttcggtgta accactgatg agacgctacg cccgtcgcag
    11101 gcagggcaaa ccattgagaa cctgtttgcc atcggttcgg tgctgggcgg atttgatccc
    11161 atcgcccagg gatgcggcgg cggtgtttgt gccgtcagtg ctttacatgc cgctcaacag
    11221 attgcccaac gcgcaggagg ccaacaatga acgacaccag cttcgaaaac tgcattaagt
    11281 gcaccgtctg caccaccgcc tgcccggtga gccgggtgaa tcccggttat ccagggccaa
    11341 aacaagccgg gccggatggc gagcgtctgc gtttgaaaga tggcgcactg tatgacgagg
    11401 cgctgaaata ttgcatcaac tgcaaacgtt gtgaagtcgc ctgcccgtcc gatgtgaaga
    11461 ttggcgatat tatccagcgc gcgcgggcga aatatgacac cacgcgcccg tcgctgcgta
    11521 attttgtgtt gagtcatacc gacctgatgg gtagcgtttc cacgccgttc gcaccaatcg
    11581 tcaacaccgc tacctcgctg aaaccggtgc ggcagctgct tgatgcggcg ttaaaaatcg
    11641 atcatcgtcg cacgctaccg aaatactcct tcggcacgtt ccgtcgctgg tatcgcagca
    11701 tagcggctca gcaagcacaa tataaagacc aggtcgcttt ctttcacggc tgcttcgtta
    11761 actacaacca tccgcagtta ggtaaagatt taattaaagt gctcaacgca atgggtaccg
    11821 gtgtacaact gctcagcaaa gaaaaatgct gcggcgtacc gctaatcgcc aacggcttta
    11881 ccgataaagc acgcaaacag gcaattacga atgtagagtc gatccgcgaa gctgtgggag
    11941 taaaaggcat tccggtgatt gccacctcct caacctgtac atttgccctg cgcgacgaat
    12001 acccggaagt gctgaatgtc gacaacaaag gcttgcgcga tcatatcgaa ctggcaaccc
    12061 gctggctgtg gcgcaagctg gacgaaggca aaacgttacc gctgaaaccg ctgccgctga
    12121 aagtggttta tcacactccg tgccatatgg aaaaaatggg ctggacgctc tacaccctgg
    12181 agctgttgcg taacatcccg gggcttgagt taacggtgct ggattcccag tgctgcggta
    12241 ttgcgggtac ttacggtttt aaaaaagaga actaccccac ctcacaagcc atcggcgcac
    12301 cactgttccg ccagatagaa gaaagcggcg cagatctggt ggtcaccgac tgcgaaacct
    12361 gtaaatggca gattgagatg tccacaagtc ttcgctgcga acatccgatt acgctactgg
    12421 cccaggcgct ggcttaaact cccttctgat gcccggtaag catgtggtta ccgggcattt
    12481 ttgcgtacac gattccgtgc ccaatgtatg cgttgcaacg cagtgaaaat tcctctgaaa
    12541 acgtctcgca aaggctgaaa ctggcagatg tcaaaggcct gggataaccg taatgtcgcg
    12601 tcatcataaa tatcaggtga cggacaacca tgaccgaatc aacaacctcc tccccgcatg
    12661 atgcggtatt taaaaccttt atattcacac ccgaaaccgc acgggatttt ctcgaaatac
    12721 atttaccaga accactgcgc aagctttgca acctgcaaac cttacgcctg gaacccacta
    12781 gttttattga aaaaagttta cgcgcttact actcggatgt tttgtggtcc gtggaaacca
    12841 gcgacggtga cggctatatc tactgcgtga ttgaacatca aagctctgca gaaaagaata
    12901 tggcttttcg gccaatgcgc tatgccactg ccgccatgca gcgtcaccta gataaaggct
    12961 atgacagagt tccgctggtg gtgccattgc tgttttatca tggcgaaaca tcgccctacc
    13021 cgtactcact taactggctg gatgagtctg acgatccgca acttgcccgg cagttgtaca
    13081 ccgaagcttt tccgttggtg gatatcacca tcgtacctga cgatgagatc atgcaacatc
    13141 ggcgtatagc tctgctggaa ctgattcaaa agcatattcg cgaccacgat ttaatcggca
    13201 tggtcgacag gatcaccacg cttttggtta gaggcttcac taatgacagc cagctacaaa
    13261 cactgtttaa ttatctgctg caatgcggcg atacctcccg tttcacccgt tttattcagg
    13321 agattgccga acgttcacca ctacaaaagg agagattaat gactattgct gaacggctac
    13381 ggcaggaagg gcatcaaatt ggctggcagg aaggtatgca tgaacaagcc attaaaattg
    13441 ccttgcgcat gctggagcag ggctttgaac gtgagattgt gctggcggca acccaactca
    13501 ctgatgctga tattccgaac tgttattaaa aggagaggga gagattgatg actattgctg
    13561 aacggctacg gcaggaagga catcaaattg gctggcaaga aggtaaatta gtaggtttgc
    13621 agcaaggtaa attagaaggt ttgcaggaag gtatgcatga acaagccatt aaaattgctt
    13681 tgcgcatgct ggaacagggc attgatcgtg accaagtgct cgcggccacc cagctaagcg
    13741 aagccgatct ggcagcgaat aaccactaat taacacaggc ccacagccga tccccatggg
    13801 cctttgatat caataactac cttttatgcg tggcccattt ttgcctgatt taaacatcgc
    13861 cagtcgttga tccagggcat cgctgtagag catcgtgtca acgccaacag cgacaaagtt
    13921 cgctccccac gccaggcatt gctgcgccat atcaggagcc acagccagaa aaccagccgc
    13981 tttacccgca gcacggatcc gccgaatgct ggtttcaata attcgctgca cttccgggtg
    14041 cccggcgtta tccgggtagc ccaacgacgc agaaagatcc gctggtccaa taaacacgcc
    14101 atcaatccct tcgacgtcga ggatctcgtc caggttatcc agtgccgttt tactttccac
    14161 ctgcactaac agacaaagcg aatcgttgac ttgcgccatg taattctcaa tgcgtcccca
    14221 gcgcgcagcc cgtgccacac tggccccaac accacgctca ccgtagggag ggttggacaa
    14281 attttgccgg agggggaatg tgccggtgca gtaaaccggc acagagtggc gaaatcagac
    14341 ggataaagtc atggactcaa caacctcaat ccagccatgc tcactagcga tgtcctggcc
    14401 gtttaaccaa cggcgtagca tattcagtgc catcattgcg cacacttcct gacggatagc
    14461 caggctgtag cgtgtagtgc tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc
    14521 tagcgcaaag ttgagatgct cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa
    14581 atggttggcc cgccgttctg taatccagtg cgcagtttgc gccagggttt cttcctgtga
    14641 aggaatcact tcacacgcca gcaatggagc acctgcgcga gaaagttgca aagccaataa
    14701 accaccggtg aattgctcgc tcaacgtcag gctgaactgg cggttttgca attcgcgact
    14761 gatctgcgcg ggcagtcctt cggtgccttc gaaaatcacg ctctgtccgg caacacgttt
    14821 aacgtccagc cacagttttt ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag
    14881 ttcgatgatc ggcattgagg agcgatagcc cattgttacg cccggcggca gttgtagagt
    14941 gtccaggctt tgtgccagat cgctttccga acgaccaaag gtagttaaac gcagacaaac
    15001 cggcggctgt ggtaaagaaa aacgctcgcg caggcgcggc aggatttcgt gctcgaccat
    15061 caccttaaat tctgacggta cgccgggagt aaagaacatc aggcaacgat taagctgcac
    15121 ggcaaaacca caggcggtgc ctaccgggtt attgataaat tcagcactgg caggcagctc
    15181 cgcttgttta cggctgctcg gtgccattac tcgtccacgt tcgtggaaat agcgttccat
    15241 ctctttgagc caggcttcat gcagcacaag gccttcacct tttgctgtcg cagcggcgag
    15301 tgcgcttaaa tcatcgctgg tcggtcccag cccgccgtta acgatcagca catcggcgtg
    15361 ctgactacgt tcgcgaagaa tggtgactaa gtcatcaagg ttatccccca ccgtattgcg
    15421 gcgagataat ggcagcccct gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc
    15481 aacgatttgc ccgtataaca cttcatcccc ggtggatagc atttccactt ttaacatggt
    15541 tatctcctgc ttttgttatc gaatcactat aacgcaaagg gagataaggc gtagtaagaa
    15601 actgaatgcc gcgccggaag ggatcagaaa ctggcgttaa ccccaacata caggccgtca
    15661 gccacagcgt tgtcgcggtt accgtcttta cccgacagat tcaggtagcg ataacccgcc
    15721 tcaatactga ctggacgcat aatggtgtaa cgcgcgccag cattcgcttc ctcataactt
    15781 tgaataccgc tggagagcga atccggagag tagtaatact cgccaaacaa acggaagctg
    15841 ttgccaattt tccactgcaa accacctcct accgctgcgg cataaccttc atcgccgtaa
    15901 ttcgggttgg tgtacacgcc ttttccgcca acggtcgcca ttaacggccc gagaggcaga
    15961 ttcaacccca gccccacgcc cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg
    16021 cttaaagcca ggcccgtcga ttcagtccca aaaccgacac caatattggt gtattcttta
    16081 cccgcctggc cgctgatgct cattgcattg accgatgcag aaaccaacag cattccggcc
    16141 agacctgcta gcgcaatttt tttcattatc ccctttccca caagcaatta atcaaaattc
    16201 ccaaagctgc gggattgtac tgcctggcac agaggattca atgagcgaaa aaagggatct
    16261 agtcacttat tgtaactaaa tacttcttaa ataattaaac gccgaaaaaa taaacacact
    16321 ttaccgatac acacaaaaat aataataagt gcagttatca ttgcaaaaga gattactgtc
    16381 actttcacat atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac
    16441 ccctgtgcga caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct
    16501 gtgcaaaatg gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg
    16561 cgtggagtct ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg
    16621 agaacagctg cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa
    16681 gacatatgca gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt
    16741 ttctgccaac cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc
    16801 tgaagatctg gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg
    16861 tcttctttaa caatgatgac agccatcact ggctgtcagg ttgaaaattt agtggttaac
    16921 gaattcccca tccagataaa ctttgccttt ttccacatgc atgactaaac catccagata
    16981 atcaggttta aatatcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg
    17041 ggtgaaaata accatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata
    17101 aatctcatta ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa
    17161 ccaggtcgcc gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat
    17221 gtcagcgcta aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc
    17281 tgttttatct gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa
    17341 aacgacaacc gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa
    17401 accattgcta ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa
    17461 aatgatgaaa tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc
    17521 acctgtagac aagcgcaacc agacaattac cgtgaaattg agcgacattt ctggcgataa
    17581 ttagcagttg gtgtaatatt aaaaatccta cgatgtcggc aaaatgcctc aaatttttgc
    17641 caaatgcaaa gcctaaataa gaaaaaatat aaaaatttca atatttacgt ctaatattag
    17701 tttcttaagg ttaagttaat attctatcct taaaatttcg ctccaaatgg caaaatatac
    17761 acaacactct ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat
    17821 gtcagaattt ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa
    17881 agaggttctc gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc
    17941 cttttgccag ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat
    18001 gcatatcacg ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct
    18061 gacctgggtt tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga
    18121 tgtcgaccgc gatacgctga tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg
    18181 cactaaagcc atcattccgg tgcattatgc cggtgcgcca gcagatattg acaccattcg
    18241 cgccattggc gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac
    18301 gtattacaaa gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat
    18361 taaaaatatt acctgtgctg aaggtggcct gattgtaact gataatgaaa accttgcccg
    18421 ccagctacgg atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacaaacctg
    18481 gggccgtgca ccgcaggctg aagtcttaac accgggctat aagtacaatc tgaccgatat
    18541 taacgccgcg attgccctga cacagttagt caaattagag cacctcaaca cccgtcggcg
    18601 cgaaattgcc cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct
    18661 tcccgcctgg ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg
    18721 ttgtggtatc agtcgcgatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg
    18781 gttacatttc cgcgccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc
    18841 gttaccgaat accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat
    18901 gactaccgcc gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc
    18961 catgtttgaa atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca
    19021 ggaaagctta ccggaattaa tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga
    19081 gtatgagatc ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga
    19141 agcctcacaa gcggagaaca gccatattgt gtctattttg cttaaccgca attacgggca
    19201 acattcagcg attatggcgg gattcagtca cgttactggc gacttaatta ttacccttga
    19261 tgccgatctc cagaatccgc cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg
    19321 ttacgacgtg gtagggactg tacgccagaa ccgccaggac agctggtttc gtaaaacagc
    19381 ttcgaagatg attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactacgg
    19441 ttgtatgctg cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg
    19501 cagcaccttt atcccgattc tggcgaatat cttcgcccgc cgtgcaattg aaattccagt
    19561 acatcatgcc gagcgtgagt ttggtgaatc caaatacagt tttatgcgcc tgattaattt
    19621 gatgtacgac ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg
    19681 cagcattatt gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac
    19741 cttcggacca caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt
    19801 tattggcgct cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac
    19861 cgatgtccgc gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga
    19921 aaatgaataa tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa
    19981 gctctgctgg ctgccggtta cgaaattagc gccattttta cccataccga taatcccggt
    20041 gaaaaagcct tttatggttc ggtggctcat ctggcggcgg aaagagacat tccggtttat
    20101 gcgccggata acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcaccagag
    20161 gtgattttct ctttttatta tcgccatctt atttgcgacg agatttttca gctcgctccc
    20221 gcaggtgcat ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg
    20281 aactgggtgc tggtgaacgg tgaaacggaa acgggcgtta cattgcaccg aatggtaaaa
    20341 cgtgccgatg ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc
    20401 gctattacgc tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta
    20461 cccgccatta aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt
    20521 tttggtcgca gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg
    20581 cacaacatgg tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat
    20641 cagaaattca ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg
    20701 agcgtgattt ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc
    20761 accggacagg cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc
    20821 ctggtgcaag gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg
    20881 gtactcatcc tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc
    20941 gaagatcatt atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg
    21001 aatcatccac attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag
    21061 tatcacgtca aaaaatgtga tttcgtcttg ccgctggtgg cgatagccac gccgattgaa
    21121 tatacccgca acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc
    21181 cgctactgcg ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg
    21241 atgtgtagcg ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat
    21301 aaaccacgct ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat
    21361 ggcgaaaaag agggtttaca gttcaccctc ttccgcccgt ttaactggat gggaccacga
    21421 ctggataacc ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc
    21481 aatctggtag aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt
    21541 actgatatcc gggacggtat cgaggcgttg taccgcatta tcgaaaatgc gggaaatcgc
    21601 tgcgacggcg aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg
    21661 ggcgagatgc tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt
    21721 gcgggctttc gcgttgtcga aagtagcagc tactacggca aaggatatca ggacgtagag
    21781 catcgtaaac cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat
    21841 atgcaggaaa ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat
    21901 aaaccatcat gaccaaagta ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg
    21961 aaggcgtgcc gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt
    22021 tcagcgtcgg cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt
    22081 tgtggaagat gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag
    22141 gtacggcctg gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg
    22201 ctaaacatca cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg
    22261 gtaactggga tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag
    22321 agattatcgg tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg
    22381 tgatcgaagc aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatgc
    22441 cgttccgtcc actgctcgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac
    22501 ccacctggga tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac
    22561 tcaaccgcat cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag
    22621 gctgcgctta tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga
    22681 cattttgccc tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg
    22741 gaaatattgc cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat
    22801 gaaatcggta cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc
    22861 gatcagcacg cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga
    22921 aatgctggca tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa
    22981 acccattgcc ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt
    23041 tggtgtgcgg gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt
    23101 tactctgcgc ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc
    23161 attgtttatt gtctatgcca tcggcactta tgccgtgctc gatccgttta ttgcattctg
    23221 gctggtggcg ggaatgtgca gcttctggct ggcaatgcag gcacagacgt ggaaaggcaa
    23281 aagcgcagga tttttactgc tgggaatcac ctgcggcatg ggggtgatga ccaaaggttt
    23341 tctcgccctt gccgtgccgg tattaagcgt gctgccatgg gtagcgacgc aaaaacgctg
    23401 gaaagatctc tttatttacg gctggctggc ggttatcagt tgcgtactga cggttctccc
    23461 ctggggactg gcgatagcgc agcgggagcc tgacttgtgg cactattttt tctgggttga
    23521 gcatattcaa cgctttgcac tagatgatgc ccaacataga gctccgttct ggtactacgt
    23581 gccggtcatc attgccggta gcctgccgtg gctgggatta ctccccggtg cactgtacac
    23641 aggctggaaa aaccgcaagc attccgcaac cgtctatttg ttgagctgga cgataatgcc
    23701 gctgctgttt ttctccgtcg ctaaaggcaa attgcccacc tatattcttt cttgctttgc
    23761 acctctggca atgctgttgg cgcattacgc tttgctggca gcaaaaaata atcctctggc
    23821 gctgcggatt aatggctgga ttaacatcgc ttttggcgtc actggcatta ttgccacatt
    23881 tgtgatctcc ccctggggac caatgaacac gccggtgtgg caaaccttcg agagctataa
    23941 agtcttttgt gcctggtcga ttttttcgct atgggcattt ttcggctggt acaccttaac
    24001 aaacgtcgaa aagacctggt ctttcgccgc gctttgcccg ctggggctgg cgttgctggt
    24061 aggattttca attcctgaca gagtcatgga aggaaaacat ccgcaatttt ttgtcgagat
    24121 gacacaagaa tcacttcagc caagccgcta tattcttact gatagcgtcg gtgttgccgc
    24181 aggtctggca tggagcctgc aacgcgatga catcatcatg tatcgccaga caggtgagtt
    24241 gaaatacggc cttaattatc cggatgcgaa agggagattt gtcagcggtg atgagttcgc
    24301 aaactggctt aatcaacatc gtcaggaggg gattattaca ctcgtgcttt cggttgaccg
    24361 cgatgaagat atcaacagtc tcgccattcc gcccgcagat gccatcgttc gtcaggagcg
    24421 tctggtgctg attcagtatc gtcccaaatg atctggctaa cattagtctt tgccagcttg
    24481 cttagcgttg ccgggcagtt gtgtcagaaa caggcaacct gctttgtggc gataaacaaa
    24541 cggcgcaaac atatcgtgct gtggctggga ctggcgctgg cttgtcttgg tcttgccatg
    24601 gtgctctggc tgctggtctt gcagaacgta ccggtaggca ttgcctaccc gatgttaagt
    24661 ctgaattttg tctgggtgac gctggctgca gtaaaactgt ggcacgaacc ggtatcgccg
    24721 cgtcactggt gtggggtggc gttcattatt ggtggcattg tgatcctcgg gagtacggtg
    24781 taatgggcct gatatgggga ttattcagcg tcattattgc ctcagttgcg caattaagcc
    24841 tgggttttgc ggcaagtcat ctgccgccga tgacgcactt atgggatttt atcgcggctc
    24901 tgttagcttt tggcctggat gcccggatac tgttactcgg gctgctgggt tatctgcttt
    24961 ccgttttttg ttggtataaa acgctacata aacttgcgct aagcaaagcc tatgccttgc
    25021 tcagtatgag ttatgtgctg gtatggattg cttcaatggt acttcccggc tgggaaggga
    25081 ctttttcgct gaaagcacta ctgggagtag cctgtattat gagcgggttg atgctgattt
    25141 tcctgcccac gacaaaacaa cgttactgag ttttccctgc cgctgtacac tgccgttccc
    25201 actcatcggg gctataactg cttgccgaga ggactttcac cgtatgcagc ttttcacgat
    25261 taatacaata aagcgcattc tgcaaaggcg agagtaaatc ccccactttc acggcgaagt
    25321 cactcttcac ttcggcgatc atttttatcg cgccgccagc atcgcagagc ataagtacat
    25381 gtctgttatc ttgtttattg cagcacgatt ttttgtgcca gccattccat tgcattatcc
    25441 tgtttgctaa gagttttcac atcaatttac agattaacag ataaactctt tatcaacggc
    25501 aatggaatgg cagaaatgag aagtgaaacg gagggttaat tattgctgac gttgcaccca
    25561 ctcttttagc gcctgacgtg aaattttaat accgccgttt ttcagctccg gcggtaaagt
    25621 cagccagcgc accggttgtt gaaaacgagc cagcttatct ttcacccatt cgccaagatc
    25681 aacggtttgc tggtcatact ccaccaccgc taccggccga tgaccaaact ccttgtcggc
    25741 aacggaaacg ataaacacct gcaaaaccgc aggatgtgca gcaataacac gctcgacttc
    25801 ttccggctga ataccctcac cgccactgaa gaacagattg tctaaacgtc cgacaatggt
    25861 cagcttgcca ttatgcatct caccgcgatc gcgcgtagcg taccagcctt cgtcattaac
    25921 cagtgaaacc agttgcccgt tacgccagta accttctgcc atactggcag cccgcagcca
    25981 cacttcatta ttaacgattt tcacttcccg acccggcagc ggcaaaccaa cgtctgccag
    26041 gacgtcggct tctttcgcac ataccgtgga ggcaaactcg gtcagaccat agccgcaaaa
    26101 gcaacgaatc ccctgctcgc gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc
    26161 aagtaacacc gctttcaggg aaacggaact gcggttaacc agcaaacgcc agagttgtgt
    26221 tggcaccagt gaagcgtgag tacagcctgc cagcatttgc tccaatggct gtttatcacg
    26281 caccgtcatc cgcgcaccag cgtataacca gcgccacata attccctgac cggagacgtg
    26341 aaacagcggt aaagagagca accagtcatc gtgatcgcca aacggaatca gcgataacac
    26401 accttgcgca ctggcaagat gggcctgata agtatggaca gcggctttcg gcaaaccggt
    26461 agaacccgag gtcaacgtca ttgagcacag acgcgtcggc tgccacgcag cggcatgtac
    26521 gccttcaacc agctgaatgt gcagcgacgt taatgccgga aacgtgtttt ccccatccgg
    26581 caccagcgca aattgcagcg tcagattggg cagcaattct tcaagcaacg gttgcggcag
    26641 ctgagggttc acgggcaaca cccgcgcccc gcattgcagt aacgccagcc aggcgagcag
    26701 cgtttgcggc gtattccacg cccgcaacat cacgccgctg ccctcaacca caccctgcgc
    26761 cgcaaagccc gtagctaact catcgacgcg agcacaaagc tcgcgccagt tgagttgctc
    26821 gtcattaaga cgtaaggcga tggcttctcc ccgcacttgc cgccagtgac gccacggcca
    26881 gtcagagaag atcataacaa ctgctccagt acatcagcgt ccactaacgg cagtgggctc
    26941 cccggccagc gacgaacctg ctgcgcctgc atcagatcca gcgtgtccag tcccggaatg
    27001 gtgtctggcg ttaaccaggc agcaatccgc gccagttgcg ttagacctaa gctcgactca
    27061 atggaagaac tgatcaccgc cgtcagcccc agcgcatgcg ccgcctgtac ctgctcgcgt
    27121 actttttcca gactgcccgt gagcgtgggt ttgataacta ccgcgcgcac gccctcttca
    27181 gccacaaagg caaaatccgg ctcgcgcagg ctttcatccc aggcaatggc aatgccggtt
    27241 tcacgggcaa acgctcgcga atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg
    27301 cggtggcgat aatccgggtt aacatatttg gcaaactgct gacctttcag cggtgtccag
    27361 gcgcgatttg cgtcaagacg caaatgcaga tccggaattg cctccagcaa cagattcacc
    27421 accatgccgt cgcgcaccgc ttcgtacaat cccactttga ccttcgccac tttctcgccc
    27481 ggcatatcgg caagtttgag gatcagatcg tccggatcgc cattacacag cggtgccgca
    27541 cggtagttgg ctgcttgcgg caacgtatct gtcagttctg ccaatgcaca gcttacgcca
    27601 aaggccacgg aaggcatctg cggtagctcg caatcgcctg ccagccagtt atttacccag
    27661 gcaagcagca cgctttgcgc ctcttcccag ttttcctggc tgaagcccgg cagtggggag
    27721 atctcccccc acccttcgcg ctcgccctca cgcaggcaaa cgtacagccc gtcgcgggtt
    27781 tttaaccgcc tgtcgcgcag aagcaccccc gcgtccatgg ggatctgcca gcggtatacc
    27841 tgcgcgctac gcattacgga ttccgtttaa atttgctgaa atcaggctga cgtttctggt
    27901 tgaaagcgtt gcgaccttcc tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc
    27961 ccgccagctc ctgcagcccc gcctgcccgt cacagtcggc gttcagcgca gctttcaggc
    28021 agcgcagcgc catcgggctg ttttgcagca tttcgcggca ccaacggacg gtttcttttt
    28081 ccagatccgc cagcggtacc acggtgttga caaggcccat atccagcgcc tgttttgcgt
    28141 cgtactgacg gcacaggaac cagatttctc gcgctttttt ctgcccgacg atacgagcca
    28201 tgtaggaagc gccccagccg ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga
    28261 tggcattatc tgctgcgata gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga
    28321 tggagtagcc agccaccatc gcgacaaccg gtttcggaca ggtacggatc tgacgctgga
    28381 agtccagcac gttcaggtga tgtacgccgg aatcatcttt atagccgccg taatcaccac
    28441 gcactttctg gtcaccaccg gagcagaacg ctttatcgcc tgcgccagtc agaatgatca
    28501 cgccgatatt gtcgtcataa cgcgcatctg ccagtgcctg gatcatctct tttaccgtca
    28561 gaggacggaa ggcattgcgc acctgcggac gattaatggt gatttttgcg ataccgtcgg
    28621 tggatttttc ataacgaatg tcctcgaaac cttcggagca gtcgtgccat tcaaccggtg
    28681 cgtaaggcat tgcttcatca ggataaatca tattgtgtcc tttagtcaaa aacgcaagat
    28741 ctgcgccaga cttgcgatta cgccagcggg attttcccga tgcgcgttat gtccggcgcg
    28801 aggaatgaca tggcagtcgg cagccagttc cgccgctagg gcgcggaatt tgctgtcacg
    28861 ttcaccacat aaataataaa acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg
    28921 ctggacggcg agagaagtcg cctccagcat ggcggcaagg gttgcgccat tattgttgct
    28981 gcgcagcgcc accagctccc ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg
    29041 ataccagtcg gcaaataccg ccgttaacgg ttctgtgcga aaacgctgcg cccattggcg
    29101 atcggaacgc tgacgttccg cacgttgttc agcattttgc agccccggat gcccgccttc
    29161 gataataacc ccacaaagcc ccgccagccc ctggcaagcc gccatcatcg ccacccgtcc
    29221 gccaagcgag taccccacca gccagaagtc aaggatgttg taactaacca aggttttacg
    29281 cagtaagtcg gtgacatcat caaatccatc gacgctaatc gccgccgaac caccatgacc
    29341 tgggagatca acatacaacc gtgagtagtc ggcaaacgcc tcgcccactt cttgccattc
    29401 gtggcaatcg ccggaaaaac cgtgcaaaaa caccagccag ggtaaacctg gttttccgtg
    29461 ttttgcctgc gcgtgcagga tcataaatgg cttacctgcg ccagaagttg ctggagcgtt
    29521 tgcgcaccat cggtgtcgtt aaccaccatt tcaatcaccg tggtggttgg cgtgcgccag
    29581 gcgtcggcaa atgccgtttc aagttcctgc cagttttgcg gacgatgata tttcagctcg
    29641 aacatcgcgg cggcgtgctc aaaatggacg ttttgcggca tcagatagaa acgctcacgc
    29701 tcgctttgcg gcgttggcaa cagcgagaaa atttgcccgc cgttgttgtt caccacaatt
    29761 aataccagcg gcgcagaaac ctgacgcaat aacgccagcg cgttgagatc gtaaagtgcg
    29821 gagagatcgc ccacaatcgc cagcgtcggt ttgccgcttg cccgctgaac gcctgcggcg
    29881 gtcgaaagca gtccgtcgat accgctggcc cctcggttgc tgtacaccgg gtaacctgcc
    29941 ggaagttgcg aaagcgcatc aatcagacgt accaccaggc tgttaccaac aaacaattgc
    30001 ccctgttcag gcagatagtc gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatca
    30061 cggcgggcaa taaccgcctg cattgcctgt tccgccaggc gcgggatttc aacgcaccag
    30121 ggctggcgtt tttctgccgg atgcagctcc agccagtcgg caatattgcc aattaagcga
    30181 cgtccgcgat ggtgtgccgg atcaagtcgc ccttcaatgt catcaacaat ccagtactct
    30241 tctggttcac agcttgcctg ccattgcagg agccgtttcc ccgtcaggct gcttcccagt
    30301 tgcaccacaa tttgtgcctg ctgcagctcg ctggtcgctt tggcattgcc taaccaaaga
    30361 tcggcacacg gcagcggctg cccggtttgt gacagcacat cgccaatcag cggccagcca
    30421 agagtttgcg cccacagggc aacttttttg ccctcttccg cactcatgcg cccggcaacc
    30481 accacgccgc gcttttgtcg ccagaagaac cagtcgcgct gtttttcact ttccagacga
    30541 ggcgcttcac gcagccacgg tttgtcgtcc tgccaccagt cacccagacg ctgttgccag
    30601 ctaagcccgg tatcgtccat ttcgccatac agcggttcag caaacgggca gttgatatgg
    30661 actccccccg catgaagcgt accgagagcg tggtcgatgg tagaaaccag ccaacgtgcg
    30721 gggatatcct gggtcgggcg cggcaatgaa atactgtgcg tggggtgaga ggcgaacatt
    30781 cccggctggc gaattgcctg attcgcgccg cagtcaatta gctccggcgg gcgatcggcg
    30841 gttaagagaa tcagtttttc tccggttaac ccggcttcaa tcagtgccgg atagagattt
    30901 gccaccgccg tgccggaggt cacaatcacc gccaccggct gcttgctgac tttcgccagc
    30961 cccagcgcca gatgccccaa cccacgctca tcgaaatggg tgtggtgaat gaatgcggaa
    31021 ttctccgccg ccgctaacgt taacggtgta gaacgcgagc ctggggcgat acagatgtgt
    31081 ctgacgccgt gacgcgttaa tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg
    31141 cttactgaca tgagtttgtc cggtatcaat attgcggcta agtataagga gctacaaaaa
    31201 tagattattg atatgaatcg gtaatgatgc gactcattac tattccattt gtaataaagt
    31261 acgcagccct gccgctttgt tgtcgatttc ctgccactct tgctcggggt cggaaccacg
    31321 gacaatgccc gcgccagcat ataatcgcac gacattgccg ctaatttttg ctgagcgcag
    31381 ggaaacgcag aattcgcttt gttgtaatga gagatagccc gcagaaccgg cgtaccattc
    31441 tcgggtgaac ggttcgtgac gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc
    31501 aactgctgcc gttggctgca actgatgtaa acagatcaca tcatccgctt tgttgagtga
    31561 agtccagata cagcggcgaa gatgctgcac tttacgcaga cgcagtacct gcggcggtaa
    31621 aacatccagc gtctgggtat cggcctgtaa tcgttggcag atatcttcca ccaccagcat
    31681 gttctcgcgc tggtttttat catccgccat cagccactct cctaactgct gcgcctgctt
    31741 atcatcagga tgatttgcta ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg
    31801 ccgccgccat aaccgttccg gtgaagagcc aagaaaagca ttttcgccat caaaggccat
    31861 gtaaaaatgg tagcaattca gattcagtcg acgactggca gccatcatcg ccgccgcgtt
    31921 gaccggactt gcgaaatgca ggtcagttgc ccgagcgagc accactttgt cgagctcacc
    31981 ttcggcgatg gttttcgttg ccagttcgat taattgcgtc cagcccgttt tgtccggcca
    32041 gtgttgttct cgcgtggtgg ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt
    32101 ggcgataaat tcttttgcct gaatcgcatc gtgctgaagg gagctttcgc tgaatagcgt
    32161 caaccgcagc gtggctttac cgccacagcg tcgccattcc aggcggggta aaagtaaatt
    32221 gccctgcgac gggtcaaaag cattcagccc ccaaatgcgt aagtcggcgt gttccgggtg
    32281 ctggcgaaga aaacgttgtg cctggtccaa cgacgtaaaa cgggtaatcg cgcccaggac
    32341 ggcagcttct tcatcaccat tacgttgttg ccagtagaat tgcgggtacg tttgctgact
    32401 ggccagccag ctcaaggcat caaaagcgtc tttgagaggg aaaggaatat cgataacccg
    32461 aatgccgggt gtcgccggaa tctcttgcga caaatggcgc agtagatttt ccagcgccgt
    32521 agtaagtgat tgcacgcgga cctctccctg ttaaaagcct cacattatac ggggtactac
    32581 aaaaaaaatg cagtaccccg gtgtagggag gttttaacgg cgtgccagca acagtcctag
    32641 taccagcccg acggccgcgc ccacaccaat tccttgccag ggtttttcgt ggacgtagtc
    32701 atcagcacga taaacagcct gcttcgcccg atagtaataa ctgtctgaag cctggctaac
    32761 ccgttttttc acatcatcca gcgctttttc tgcacgcgct ttcagctcaa catatttctg
    32821 atcggcgggc tcgccagagg agcggagcac ctcttccagt gtttcactaa gcagcgtcag
    32881 gtcgtcatcg atacgtgtat caccaaactg attagacatt ctcgttctcc atttgcgtaa
    32941 aacctgtcac taactataga caagagttgt tgattacgcc tgaattacct cgcgtgccat
    33001 cccaatgtgt gggataccat cttcttcata gacctccgtc acagggataa aaccgaaact
    33061 ctggtagaag ttttgcagat gcgcctgcgc cccgaggtaa acaggcttat caggccagtg
    33121 atgcgtacat gtttcgagtg ttttactcat cagctgctgc cccacttttt caccacgcaa
    33181 cgcttcactg acaatcaccc gacctataac gacaggctca agatcatcat cacttttcag
    33241 aatcctcgca tacgccacca gttcatcatt tttccaaccg agaatatggc gattatcccc
    33301 cgtcaaatca tcaccatcga tatcctgata agggcaattc tgctcgacaa caaacaccgc
    33361 gcaacgcagt tgtaataagg cataaagctg ggaaaccgat aattcagagt gatgcagatc
    33421 ttgccattca atcataatgc tctcctttta gatgtatgcc ttatactagc cggaatattt
    33481 tttgaaacgt gatgaactca tggaattaat ttttttaggt acttcagccg gtgtgccaac
    33541 ccgcacgcgc aatgtcacgg caatattgct taacctgcaa catccgaccc agagcggact
    33601 ttggttgttt gactgcggtg aaggcaccca gcatcagcta ctgcataccg cctttaaccc
    33661 tggaaaactg gacaagattt ttatcagtca ccttcatggc gatcatcttt ttggtttacc
    33721 cggcttgctg tgcagtcgtt ctatgtcagg cattatccaa cccttaacga tttatggtcc
    33781 gcaaggtatc cgtgaatttg tggaaaccgc gcagcggatt agcggctcat ggaccgatta
    33841 tccgctggaa attgtcgaaa ttggcgctgg cgaaattctc gatgatggcc tgcgcaaagt
    33901 aaccgcttat ccgctggaac acccactgga atgttatggc tatcgtattg aagaacatga
    33961 taaaccgggt gcattaaatg cccaggcatt aaaagctgct ggcgtgccgc ctggcccact
    34021 gtttcaggaa ttaaaagcgg gcaaaacaat cacgctggaa gatggaaggc agattaacgg
    34081 cgcagattac ttagctgctc cagtgccagg taaagcgctc gctattttcg gcgataccgg
    34141 cccctgcgat gccgcacttg acctggctaa aggtgtcgat gtcatggtgc acgaagcgac
    34201 gctggatata accatggaag ccaaagccaa tagtcgcggc catagctcta cacgccaggc
    34261 tgcgacacta gcccgtgagg ctggagtcgg caagctaatc attacccacg tcagctcgcg
    34321 ctatgatgac aaaggttgtc aacacctgtt acgtgaatgc aggtcaattt tcccggcgac
    34381 tgaactggcg aatgatttca ccgtgtttaa cgtttaactc tgcttattat taaaaacagg
    34441 gtgaaacttg ccctgttata gcaacccgcg ctacatatat ccacccacac aggcatattg
    34501 agcgattttt gatatttatt catctcctta cgcactatat tcagagcaat aagttgcctc
    34561 ttttcatctt gattcagcac aacctttgca actaaaagga gcccatgatg gttacagttg
    34621 tcagcaatta ttgtcaatta tctcaaaccc aactcagtca gacatttgca gaaaaattta
    34681 ctgtgaccga ggaattactg cagtctttaa aaaaaacagc gttatccgga gatgaagaaa
    34741 gtattgagtt actgcataat attgcgttag gttatgatga atttggaaaa aaagctgaag
    34801 atattcttta ccatattgtt agaaacccaa caaatgagac cctatcgatt atccgactta
    34861 ttaaaaatgc ctgtttaaaa ttatataatt tggcacatac cgcaaccaac tcccacctta
    34921 aaccaactgg tccggataat tcagatgttc tcctgttcaa aaagctattc tccccttcga
    34981 aattaatgac aattatcggt aatgaaattc ctcttatatc tgaaaaacag tcgctttcaa
    35041 aggtgctttt aaatgatgag aataatgaac tgagtgatgg tacaaacttc tgggataaaa
    35101 atcgtcaatt aaccacagat gaaatagctt gctatcttca gaagatcgcc gctaatgcaa
    35161 aaaatactca agtcaattat cctactggtc tctacgtccc ctactccacc agaactcacc
    35221 tggaagacgc tctcaatgaa aatattaaga gcgatccatc atggccgaat gaagtccagt
    35281 tattccccat aaatactggc ggacactgga tattagtttc gctacagaaa atagtaaata
    35341 aaaaaaataa taaactacaa ataaaatgcg tcatattcaa ctcattgcgt gcactaggcc
    35401 atgataaaga aaattcactt aagcgtgtca ttaatagttt taattctgaa ctcatgggaa
    35461 aaatgtcgaa taataatata aaagttcatt taaatgaacc agagataata tttttacatg
    35521 ccgatcttca gcaatactta agccaaagtt gcggtgcgtt tgtgtgcatg gcagcccagg
    35581 aagtgattga acaaagggaa agcaattctg acagcgcccc ctatacgtta ttaaaaaact
    35641 atgctgacag atttaaaaaa tattcagcag aagagcagta cgaaattgat tttcaacatc
    35701 gactggtaaa cagaaattgt tatttagata aatatggcga tgcacgaatc aatgcttctt
    35761 atacacaatt agaaataaaa cactcacaac ccaaaaatag agcatccggc aaaagagtga
    35821 gttaacatca ttgttaatca attcgaccag tcagtaaata tgctgactgg tcgaacataa
    35881 tttgctaaaa agtcatcact gactgatgtc agtcccacca tctgccagtt caatctacgg
    35941 gtatttccag cgtatcgcgg cacgcgattg ctggtctgtt gatgtagtgg attgtagttg
    36001 ctgacttttc ttaccctcag atttctgccc cacttgtcaa ttcaatctgc caataaataa
    36061 cataattata tttttattcc tcacagaaat tcctttttaa aaatagcgaa cccactgtaa
    36121 aaaacacttt ttgtatacct ctaccaagcg taataaaata aacgctcata attcattata
    36181 aataataatg aaataacgcc ttttattgat aatttacata tgaaaaaaat tttccttttg
    36241 ttatattatt tctgttttca ctccctctga ttatttttac caaccctggg tcactgcatt
    36301 atcaccaacg ccccgacacg taagtcctga gcaattagaa aaaacagttc gttatcttac
    36361 acaaactgtg catccacgta atgccggcaa tattgataat ctgaataggt ccgcagagta
    36421 tataaaagag ttctttgtca gtagcggtgc cagagttacc gcgcagggcg tccccgattg
    36481 gctattacga ctctgcgcct ggaagccaga attaccctta cccggcaatg tcctggcttt
    36541 atcccgatca gggagacttt attgctgtgg tcggcagaat gcaggatatc aacgccgttc
    36601 gtcaggtaaa agcggcattg ttgtcatccc gggatttatc tgtttattct atgaatgccc
    36661 cagggtttat tcccggtatt gatttttctg atcaccttaa ttattggcaa cacgatattc
    36721 ccgccgtaat gattactgac accgcttttt atcgtaataa acaataccac ttgcccggtg
    36781 ataccgcaga cagattgaat tatcagaaaa tggctcaggt ggtagatggt gttataacct
    36841 tgttatacaa cagtaaataa taaaaatttc tcgaggcggg tccagaaaca gcgacatgta
    36901 cattttattt tccctcgtct gttttaagat gcgaaacata aaattcaatg gaggaaaaat
    36961 aaatgggaat gattggctat tttgcagaaa ttgactcaga aaagattaac caactgcttg
    37021 agtctactga aaaacctttg atggataata tacatgacac gctttcagga ttacgccgac
    37081 tggatattga taaacgctgg gattttttac actttgggct gacaggcacc tctgcttttg
    37141 atcccgcgaa gaatgatccg ctgagtcgtg ccgtgcttga tgaacacagc ctcgaagatg
    37201 gcatagatgg cttcctggga ttgacctgga atcaggaatt agccgcgaca atcgaccgac
    37261 tggaatcact ggatcgaagt gagctgcgta aacagttttc cattaagcga ctcaatgaaa
    37321 tggagattta tccgggagtg accttttcag aagagttaga ggggcaactc tttgcctcga
    37381 tcatgcttga tatggaaaag ctgattagcg cgtatcgacg tatgctgcga caagggaatc
    37441 atgcgctgac ggttattgtt gggtgatggt gtcagagcgc caaaaaaaac cgccgaacat
    37501 gtcggcggct ttctgactta caaactaaca gtttacatca gcggcattgc taaccgcaca
    37561 atactaatca gcggttgtgg ccatacaccc agcaccagta ccaacagtgc agaaatcagc
    37621 accacgatac cgcccgcgct gtactgccag tttgacggtg catcgcgacc cggttgttcc
    37681 ggtgcgtgca ggtacaggct caccgccacg cgcaggtagt agtagaggcc gattgccgaa
    37741 ccgacaacca cggcacccac cagccaccac aagtgtgcct ggacaccgac cgccagcacg
    37801 tagaacttac cgataaagcc cagcgtcatc gggataccgg ccagagacag catcatcacc
    37861 gtcatcactg ccgcgaggat cggacgatgc cagaacagac cgcggtaaga gaacagggaa
    37921 tcagcatccg ggccacgata cgggctggac atcaggctga ccacgccgaa cgcgccgagg
    37981 ctgctgaaca gataaccggc caggtaaacc cctaccgctt ccatcgacat ctcgccggtt
    38041 tgcagcgcaa tcagcgccac cagcagatag ccgaggtgag agatagatga gtaaccgagc
    38101 agacgtttga tgttggtctg gctcaacgcc atcaggttac cgaagatgat ggaggcaaag
    38161 gcgataatcg ccagtactac gcgaatcgct tcgctgtcac ccaccggtgc gtagaggaac
    38221 agacgcatca ccacaccgaa gatagcgatt ttgctcgccg tcgccaggaa agtggaaacc
    38281 ggcgcaggcg cgccctggta tacgtctggc gtccacaggt ggaacggcac cagagagagt
    38341 ttgaagccga ggccaacaat catcatgccg aaacctgcca gcaacagcgg ctcgttgagc
    38401 ataccgtcgc cgaggttttt acccagcgcg acaaacgaca ggtcgccaga ctgcgcatac
    38461 accagcgcca taccgaacag caggaaagaa gacgctgcgg cagaaaggat ggtgtatttg
    38521 atactggctt ccagcgaacg tttctggcgg aaagcgtaac cgaccaggcc aaacagcggc
    38581 aaagagatca gttcaatacc gaggaacaga gacgccagat ggtttgcatt cgccagcagg
    38641 atcccgccca gcgcggcaat taacaccaac aggtagaact catccttgtt gtcgttatag
    38701 ccttcaagcc acgggtagtc gaaagtacag gtggcgaggc tcgccaacaa taccagcccg
    38761 gtgtaaagca tggcgaaacc atcaacgcgc atcagcggcg taacgtccat agcgcccgcc
    38821 tggccaacaa accagagcga aaccagcgcc gcgttaagcc caataaccga gagcgtagcg
    38881 ttgaggaaat gattgcgtcg ccacgcaatg gagagcatca caaccaccac cgtcaagccg
    38941 acgatcagca acggtagcag tgcgatcagg ttttgtggag ttattgtcat ggcgatttac
    39001 ggccttgtag tagtaacgga attaacaaac cactgctgga tattgccaat cgcggagtgc
    39061 gaggtatcca gaatcggctg cggatagaag cccagcagta ccagcagcac caccagcaac
    39121 aggatcataa acagctcacg cagcgacatc cctggcagtt cctggctggc aatctggctt
    39181 ttcgctttac cgaagtaagc gcgatgcaac atcgccagcg aataaacaga tgcaaagacc
    39241 agcccaaagg tagagataac ggtaatcacc gggacaacct ggaagctgcc gaacagaatc
    39301 ataaattcgc cgacgaagtt accggtgcca ggcatcccaa gcgttgccac cgcaaagaac
    39361 agcgacagtg ctggcagcca tttcatcttg ctccacagac cgcccatcat gcgcatgtcg
    39421 cgggtatgga tacgttcata aagctgaccg cagagaataa acagacccgc cgccgacaag
    39481 ccgtgcgcaa tcatctggat taccgcgccc tggtaggcca actggctgcc ggtgtagatt
    39541 gcaatcagca cgaagcccat gtgggaaacc gaggtgtagg cgatcagacg tttgatatcg
    39601 gtctgagcga aggccatcca cgcaccgtag aagatgccga taacacccag ccacatagcg
    39661 attggcgcga actctgccga cgcgttcggg aacagcggca gggagaaacg caacaaaccg
    39721 taagcggcgg ttttcagcaa gatccccgcg aggtccacgg aaccggcggt cggagcctgg
    39781 gagtgcgcat ccggcagcca gccatgcagc ggaaccaccg gcattttgac tgcgaaggcg
    39841 atgaagaagc ccagcatcaa cagatattcc acaccactgg acattggcgt attcagcagc
    39901 tcttcatagt tgaaggtcca gacgccggtc gcattgtagt gaacaaaaac cagcgccagg
    39961 atggcgatca acatcaccag accactcgcc tgggtgtaaa tgaagaactt ggttgccacc
    40021 gtgatacgcg ttttaccgtc agaggcttta tgcccccaca gtgcgatcag gaagtacatc
    40081 ggcaccagca tcatttccca gaagaagaag aacaggaaca tgtcgatggc aaggaacacg
    40141 ccgataacgc cgcccaggat ccacatcagg ttgaggtgga agaagccctg atatttttcg
    40201 atctctttcc acgaacatag taccgccagc acaccgagca gaccggtcag cacgaccatc
    40261 agcagcgaca gaccgtcaat ggcgagatga atagagatac caaaacgcgg gatccacggc
    40321 atgtcgaatt cagactgcca ctgcggaatt ccggcggatt gcgtcagtga ataaccgccc
    40381 tgcaaccaca gttgcagcga cagcgccagc gtcaatccca tggtgatcag cgcgatccag
    40441 cgtggcacct tgacgccaaa gcgttcggtc tgccagcaca ggaagccgcc aataaaggga
    40501 attaatatta gccagggtag taacatggcg atctttattc cttgtaaaag tcccgtcagg
    40561 accggatttt caacgaattc tcacgacaga aaattctctt cggactgggg gtagtgtcgg
    40621 atgcgccgcg aaggcagctt atccgaccta cgttcttata tgcccagggg caatcccaca
    40681 atccttaact caacgcagta ccatcaacag tgccagcacc acgaccgcac cgatgctcat
    40741 ggatgccaca taccagcgca gataaccgtt ctcgcttaac agcagacctt tacctgcaaa
    40801 gcgggaaaga accgccggga tgttcatcat tgagttcagc ggatcgcgtt tcagcaacca
    40861 ggcaataccc aggaacggct tgacgaacac tttgtcgtac agccagtcaa agccccaggc
    40921 gttgtaccac caggtactca gcaggcggcc cggcgcactg ttggcgatgg aggtcaccag
    40981 agtacgttta cccagccaca gccaggctgc cagcagaatg ccgaccaccg cgaccacgcc
    41041 agaggtaatt tccagggtca acatgctgcc gtgcgccagt tccgtcgttt gcggaagcac
    41101 gccctgcagc ggcggtacaa tcagtgcgcc aacgaaggtg gaaaggatca gcagcacaat
    41161 cagcggcagg ctgtgagtta cccctttcac ggcgtgagcg tgaatttgtt cttttccgtg
    41221 gaagacgatg aaaatcatac ggaaggtgta gagcgaggtc ataaacgcac cgaccagacc
    41281 tgccaccatc agattgatat gaccattcgc catcgcaccc gcgaggatct catccttact
    41341 gaagaagccc gcagtgacca gcggcagtgc cgacagtgct gcgccgccca ccaggaagca
    41401 gagataaacc agcggaatag atttacgcag accgcccatc ttgaagatgt tctgttcgtg
    41461 atggcaggcc agaatgacgg aaccggatgc caggaacagc agcgctttaa agaacgcgtg
    41521 agtcatcaag tggaaaatcg ccgcatccca tgcctgcacg ccaagcgcga ggaacatgta
    41581 gccaatctgg ctcatggtag agtaagcgag aacacgtttg atgtcggtct gtaccagcgc
    41641 ggcaaaaccg gccagcagca gcgtaaccgc cccgacaata cccaccagat gcagaacttc
    41701 cggcgtcatc aggaacaggc cgtgggtacg ggcgatcagg tagacacccg cggttaccat
    41761 cgttgcggcg tggatcagcg cggagacagg cgtcgggccc gccatcgcgt cagcaagcca
    41821 tgtctgcaac ggcaactgcg cagatttacc gaccgcaccg cccagcagca tcagcgtcgc
    41881 ccacatcagc atgttattgc cgtcagcaaa gtgcgctggt gccagttcca ccatttcgcg
    41941 gaagttcagg gtgcccagtt cgttgtaaag aatgaacagt gcgaaagcga ggaaaacgtc
    42001 acccacacgg gtcacgacga acgctttcat tgctgctgcg ccattcttcg gatcggtgta
    42061 atagaaaccg atcagcagat aggagcacag gcccacgcct tcccagccga ggtacatcag
    42121 cagcaggtta tcggcgagaa ccagaactac catgctggcg atgaacaggt tggtgtaggc
    42181 gaagaagcga gagtagccct cttcaccgcg catgtaccag gaggcgtaca tgtgaataag
    42241 gaaacccaca ccagtgacca ccgagagcat ggtcagcgac aggccgtcca gcaccaggtt
    42301 aaaaccgatg ttaaagtcgc ctaccgacat ccacgtccac agcggctggc tgtatgcctg
    42361 ctcgccgtta gcgaagaaat caacgccgat aaaggcggtt acctgcgccg ccaggcccac
    42421 agagccaacg ccgacgatcg ccgaaacgtt ttcagaccag cgcccacggg agaacgccag
    42481 caggacgaag ccaatcaatg gcaaaataat ggttaaggca agcatgttca tccgcgcatc
    42541 tcacttactg aatcgatgtt caggttctgg cgacgacggt gaagttgcag cagcagcgca
    42601 aggccgatac tcgcttctgc cgccgcgagg ctgatggcga gaatgtacat cacctgaccg
    42661 tcggtctgac cccagtagct tccggcgacc acaaaggcca gcgcggaggc gttaatcatg
    42721 atttccagac caatcagcat aaacagcaga ttgcgacgga taaccagacc ggttaagcca
    42781 agaacgaata agattgccgc gaggatcagt ccatgttgta aggggatcat gcgtgctcct
    42841 ccgtttttct tttcgcgctg tcgtctttac gattgctcag cacttcaccc gcacgctctt
    42901 cacgaccgac gtggaaggcc acaaccagac ccgcgagcag cagcatagaa gccagttcca
    42961 ccgccagtac gtaaggcccg aacagcgtaa tacccactgc tttagcactg attggcgtac
    43021 cgtcgatacc ctgatcgtta acaccgagga tggcgtaaac aatcaccacc agcatgatgg
    43081 ccgacaaaat tgccgggcca atccacacct gcggtttcag ccactggcgt tcctgttcga
    43141 tttctgaacc gcccaggttg agcatcatca ccacgaacac gaacagcacc ataatggcac
    43201 ccgcgtagac gataatttcc agcgcaccgg cgaagtaagc gcctagtgag aagaacaccc
    43261 cggaaatcgc cagcagcgaa ataatcaggt acagcagtgc gtgtaccgga ttggtatggg
    43321 tgatcactcg caaggtcgca agtatggcta tcaggccaca gatataaaaa gcgaactcca
    43381 ttgcccctct ccttacggta acaggctctt gacgtcgata ggcttggctt cgttctctgc
    43441 ttcgccctta tctttgccgt cgattgccat acctgccatc cggtagaagt tatattccgg
    43501 gtatttgccc ggaccggaga tcagcagatc ctctttctcg taaaccagat cctggcgctt
    43561 gtattccccc atttcgaaat ccggggttaa ctgaatcgcc gtggtcggac aggcttcttc
    43621 gcacagacca cagaaaatgc agcgtgagaa gttaatgcgg aaaaattccg gataccagcg
    43681 accgtctttg gtttctgctt tttgcagcga gatacagccg accgggcagg ctaccgcaca
    43741 gaggttacag gctacgcaac gctcttcgcc gtccgggtcg cgggtcagaa cgatacgacc
    43801 acgataacgg ggcggcagat agaccggctc ttccgggtac attcgcgttt cgcgtttggc
    43861 gaacgcgtgc aggccgatca tccagatact acgaacctgg gtgccgaaac ctactaacaa
    43921 ttcttttaag gtcatggtct tattgcccct tattgcgcct gccagagaat gacagccgcc
    43981 gttaccagca agttgatcag cgtcagcggc aggcagattt tccagccgaa ggacattacc
    44041 tggtcataac gcggacgcgg taacgacgca cgaatcaaaa tgaacatcat cataaagaac
    44101 gcggttttca gcgcgaacca gatgaatggc ggtaacaacg ggccttgcca gccaccgaag
    44161 aacagcgtca ccatcaatgc agagatggtc acaatcccga tgtactcacc cacgaagaac
    44221 agaccgaact tcataccgga atattcaatg tggtaaccat ccgccagttc ctgctcggct
    44281 tccggctggt caaacgggtg acggtgacac accgccacgc ccgcgatggc aaaggtaata
    44341 aaaccaaaga attgcgggat gacgttccac acatgcgcct ggctgttgac gatgtcggtc
    44401 atgttgaatg aaccggcctg cgccaccacg cccatcaggg aaagcccgag gaacacttcg
    44461 tagctcaggg tctgcgcaga agcacgcatc gcacccagca gcgagtattt gttgttactt
    44521 gaccagcccg caaacagcac cgcgtaaacc gccagacctg ccatcatcag gaagaacaaa
    44581 atcccgatgt tcaggtcggc aaccacccaa cccggactga ctggcacaat tgcaaaggcc
    44641 agcagcagcg aggtaaaggc aatcatcggt gccagggtaa agatgacgcg atccgagaat
    44701 ttcgggatcc agtcttcttt aaagaacatt ttgatcatgt ccgcaaccag ctggagcgaa
    44761 ccgccccagc caacacggtt aggtccataa cggttctgga acagacccag caggcgacgt
    44821 tcgccaaagc tcatgaatgc cccacaggtg acaaccacca gcaggatccc caccgctttg
    44881 aggatggtca gcaggatctc aatcagttcc ggtgatatcc aactcattgt tgtgcctcct
    44941 tgagatcctc aagatgcgcg ccagccagca ccggagcaat gccggacata cccatcggca
    45001 agcccacctg ccctgccgtc agtccttcgg cgatttcaac cggcagcgtg accgtattgc
    45061 catcgtaact aaaggagacg cgtgtacctg cgttcacacc caacttcgcg gcatccgctg
    45121 ggttgagttt gatgtacggc tgcggcatac ggctctggaa gaccggagca cgctgtgaca
    45181 attcatcgct gccaaacagg tggtagtacg gcgcgatacg ccatttcccg tcctgcggct
    45241 ggaagcgtgc cggtacgctg gtgaagtaat ccagaccgtt ttcgctggtt tcaaacagac
    45301 gcacgcccgg atcgccaaag cgcagtttgc cgcccacttc gtcctggaat ttgttccacg
    45361 cctgcgggga gttccagccc ggcgcccagg caaacggcac ttgcgaacgg tgcgcggtcg
    45421 gctggttgtt accttccatc gagaaggtga acatggtgtc aatatcctgc ggctgacgcg
    45481 gctcatgaac gctgatattg gcgcgcatgg cggtacgacc gctgtaacgg tgcggttcac
    45541 gggccagttt ctgcccacga atacggaatg tcgcatccgg cgcagcatct ttgatacccg
    45601 ccagttccgg gattttcgcc acaacagcgt caattacatg gtcgagctgc gtccagtcca
    45661 cttcacggct cagcaaggtg ctgtgcagcg agtgcagcca gcgccagctt tccagcatga
    45721 cagttttgct gtcgtaataa gccggatcgt aaacctggaa gaaacgttgg gcgcgacctt
    45781 cattattaat taccgtaccg tcgctttcag caaagctggc agcggaaagc accagatggg
    45841 cgttttccat gatcgctgtg cgttgatgat caaccaccat caccagcggc gctttagcca
    45901 gcgcagcatt cacgcgggta gcagaagcgt aacgatgcag atcgttttcc aacaccacca
    45961 ccgcgtcggc gcgtccggtt tccagttcgg ttaacgcttc ttcaagcgaa ccgccaccca
    46021 taatgcccag ccccatgctg ttgacggaac gggcaatcat ggtgatacca acgtcagcgc
    46081 cgcgaccttt cagggctttc gcgacgttag ccgcagcctg aatcacctct atgctaccgg
    46141 cgttcgtccc ggagataatc aacggtttct tcgcacctgc cagtgcctgc acgatgacgt
    46201 cgattttgtt ttgcagctca ggttcgatac cgtcaaccgc tggtgcggag ttatccagcg
    46261 catgggcgat ggcaaaacct aaacgcgcct gatcttcaac cggtgcgcgg taagtccacg
    46321 ccgcgatatc atccagacga gtgtcatcaa cgttggtaac aaacagcgga tgcttcgcac
    46381 gttgaccgat gttgaggatt gccgcaatct gccagtcagc cactttctgt gctgcagcca
    46441 tttcgcgcgc tttacctttc accgcctgac gcactgccag tgcgacgcgc gcgccggtct
    46501 gggtaacgtc ttcgcccagc accagtaccg catcgtaaga ttcgatttcg cgcagagccg
    46561 gagtataaat gccgccttca cgcagcactt tcagcgccag ttgcagacgt tcctgctcac
    46621 cgtgagcgat accggtgtag aagttttctt cgcccaccag ttcacgcagc gcaaagttgc
    46681 tttccacgct ggcacgcgga gaaccaatac cgatcacttt cttcgactga cgcagaatat
    46741 ctgccgcgcc ctgcattgcc tgttcggcgt tgagggtaat gaaatcatcg ccacgacgct
    46801 gtactggctg acgcggacga tccttcaggt tgacgtaacc gtaaccgaaa cgaccacggt
    46861 cgcagaggaa gtagtggttt accgtaccgt tgtaacggtt ttcgatacga cgcagttcgc
    46921 cgtaacgttc accggggctg atgttacagc cgatggaaca ttgctggcag atgctcggcg
    46981 caaactgcat atcccattta cggttgtaac gctcggagtg cgttttgtcg gtaaatacgc
    47041 cggtcgggca aatttcgacc aggttaccgg aaaattcgct ttccagcgtg ccgtcttccg
    47101 ggcgaccgaa gtagacgttg tcgtgcgcgc cataaacgcc cagatctgta ccgtcagcgt
    47161 aatctttgta gtaacgcaca cagcggtagc aggcgatgca gcggttcatt tcgtgagaga
    47221 tgaatggccc caaatcctga ttacggtggg tacgtttggt gaaacggtag cgacggaagc
    47281 tgtgtccggt catcacggtc atatcctgaa gatggcagtt accgccttct tcacataccg
    47341 gacagtcgtg cgggtggttg gtcattaacc actcgaccac actttcacgg aactgtttcg
    47401 cttcttcgtc gtcaatggaa ataaaggtgc catcggaagc cggtgtcata caggacatca
    47461 ccaggcgacc acgcgtgtct tccgcgtttt ggtattgctt caccgcacac tggcggcaag
    47521 caccgacgct tcccagcgcc ggatgccagc aaaagtaagg aatatcaagg cccagagaca
    47581 gacaagcttc cagcaggttg tccgctccgt tgacctcgta ttctttgccg tctacatgaa
    47641 ttgtagccat tagcatgctt ccagttttct cggtcaggga ccgagcgtta atcgaaattc
    47701 ggttaccagc gctctttcag caggttcggc tgaatcccat taatcaaatg ggtattgctg
    47761 aacggctgtt tgattcccgc ctcaaattct tcgcggaaat atttgatggc gctctgtaac
    47821 ggctccactg caccaggtgc gtgggcacag aaagttttac ccgggcctaa gaatcgacac
    47881 agttgctcaa gtgtttcgat atcgcccggc tggccttcgc cacgctccag cgcacgcaga
    47941 attttcacgc tccacggcag accgtcgcgg cacggcgtac accagccgca ggactcacgg
    48001 gcgaaaaact cttccaggtt acgcaccagc gacaccatgt tgatctcatg gtcaaccgcc
    48061 atcgccagcg ccgtacccag acggctgccc gctttaccga tactttcgaa ttccatcggc
    48121 agatcaaggt gcgcttcggt caggaagtca gtccccgcgc cgcctggctg ccaggcttta
    48181 aatttcagac catcacgcat accaccggcg taatcttcga ggatctcgcg tgcggtggtg
    48241 ccgaacggca gttcccacag tcccggattt ttcacccgac cggagaagcc catcagcttg
    48301 gtgccagcat ctttactttt cgagatgttc tgataccact ccacgccgtt agcgaggatc
    48361 gccggaacgt tacacagggt ttcgacgttg ttgacacagg tcggtttgcc ccatacgccg
    48421 gaggttgccg ggaagggggg tttcgagcgt gggttagcac gacgcccttc cagggagttg
    48481 attaacgctg tttcttcccc gcagatgtag cgccctgccc cggtatggac gaacagttcg
    48541 aagtcgaaac ctgttcccat aatgtttttg ccaagcaagc ccgcttcagt ggcttcggca
    48601 atagcgcggc gcagatttac tgccgcttcg atatattcgc cacgcaggaa gatgtagcca
    48661 cggtaagctt tcagcgcaaa cgcggagatg agcatacctt ccaccagcag gtgcggcagt
    48721 tgctccatca acaggcggtc tttataggtg cccggctcca tttcatcggc gttacacagc
    48781 aggtaacgga tgttcatgga ttcgtctttc ggcatcaggc tccacttcag gccagtcgaa
    48841 aagcccgcgc cgccgcgccc tttcagacca gcgtctttta cctgattaac gatttcgtcc
    48901 ggagacagcc cggtcagcgc cttacgcgcc ccttcgtaac cgtttttgct gcggtattcg
    48961 tccagccaca ctggctgttt gtcatcgcgc agacgccagg tcagcggatg cgtttcggga
    49021 gtacggataa tgtttttcat ttataccgct ccagcagttc agggatcgct tccggggtca
    49081 gatgcgcgtg agtgtcctca tcgatcatca tgtttggccc tttatcacag ttccccaggc
    49141 agcaagttgg cagcagcgta aagcggccat caaatgtcgt ttgccctggt ttgatgttca
    49201 gctttttctc gagcgccgcc tgaataccct gatacccgtt gatatgacag accacgctgt
    49261 cgcaataacg gatcacatgg cgaccaaccg gctggcggaa gatctgactg tagaacgttg
    49321 ccacaccttc gacgtcgctt gccggaatac ccagcacatc ggcgatcgcg tggatcgcac
    49381 catccggcac ccagccacgc tgcttctgaa cgattttcag cgcttcaatg gacgccgcac
    49441 gcgggtcttc gtagtggtgc atctcgtgct caatcgcttc acgctctgcc gcactcagct
    49501 caaaagcctc ggtttgtggt tgttgattct cgtgcataat tagcggtcca catctgacat
    49561 aacaaaatcg atactgccca gataaacaat caggtcagac accaggctgc cgcggatcgc
    49621 cgccggaatt tgctgcaaat gcgcataact cggcgtgcgg atacgagtgc gataactcat
    49681 ggtgctaccg tcgctggtca ggtagtaact gttgatcccc ttggtcgcct caatcatctg
    49741 gaaagattca ttggcaggca tcaccggccc ccacgacact tgcaggaagt gggtgatcag
    49801 ggtttcgata tgttgcagcg tgcgctcttt cggcggcggc gtggtcagcg ggtgatccgc
    49861 tttgaacggg ccttccggca tgttgttgag gcactgctca agaatgcgca gactctggcg
    49921 cagctcttcc actttcagca ttacgcgggt gtagcagtca gaaacaccac caccaaccgg
    49981 gatttcaaag tcgaagtttt catagccaga ataaggacgc gccttacgca cgtcgaagtc
    50041 gatcccggta gcacgcaggc ccgcgccagt ggtgccccac tccagcgcct ctttcgcgcc
    50101 ataggcggca acgccctggg tacgaccttt cagaatggta ttttgcagcg ccgctttctc
    50161 gtaagacgcc agacgtttcg gcatccagtc gaggaactca cgcagcagac gatcccagcc
    50221 gcgcggcagg tcgtgcgcta cgccgccaat acggaaccac gccgggtgca tacggaaacc
    50281 cgtgatcgct tccaccagat cgtaaatttt ctgacgatcg gtaaaggcga agaacacggg
    50341 cgtcattgcg ccgacgtcct gaataaaggt cgagatgtac agcaggtggc tgttgatacg
    50401 gaacagttcg gagagcataa cgcgaatgac gttaacgcga tccggcacgg tgatcccggc
    50461 cagtttctct accgccagca cgtaaggcat ttcgttaacg cagccgccga ggtattcaat
    50521 acggtcggta tacggaatgt agctgtgcca ggactggcgc tcgcccattt tctccgcacc
    50581 acggtggtgg tagccgatgt ctggtacgca gtcgacaatc tcttcgccgt cgagttgtaa
    50641 aacgatacgg aaagccccgt gcgccgacgg gtggttcgga ccgaggttga ggaacatgaa
    50701 gtcctcgttt tcggtgccgc gcttcatccc ccactcttcc ggtttgaagg tcagggcttc
    50761 catctccaga tcctgtttgg ctttggtcag ctcaaacggc gagaattcgg tagcgcgcgc
    50821 cgggtaatct ttacgcagcg ggtgaccttt ccaggtttgc ggcatcatga tgcggcgcag
    50881 gttcgggtga ccgtcgaaag taatgccaaa cagatcccag gtttcacgct cataccagtt
    50941 agcgttcggg aacagtttgg tgaaggtcgg tacgtgcagg tcgttttctg ccagcgccac
    51001 cttcagcatg atgtcgcggt tacgatcgat agaaatcaga tggtagaaaa cggaaaaatc
    51061 cgcggcaggt aacccttcgc ggtgtgtgcg cagacgttcg tccatgccgt gtaagtcaaa
    51121 cagcatgacg taaggtttcg gcagtttctt taagaaatcg ccaacttcca gtaattgttc
    51181 acgcttgatc cacacaacgg gaaccccggt gcgagtcgcc tgaacagtaa aggcatccgg
    51241 cccaaaacgg ttgcgcagtt cgccaattac cggatcatca agatgatcgc gggtctgcca
    51301 ggcgggttct tgcgcggtta agtcggtcat attgttcacc attgcaaatg gtccgtggtg
    51361 actgtcggcg cagtaagctt cgcgaaatag atttaggaat aagcgaagaa aaatcccttt
    51421 gccgacaggc gcaaattaaa tctcgtcagg tgtacgcagg ttggttacgg caatgcgttc
    51481 accgcgcttg cgttcgcgct ctgattgcat attggcgcga taaacgccct gatcgccaac
    51541 cacccaggag agcggacgac gttctttgcc gatagattcc tgcaacagca tcagtgcctg
    51601 catgtacgct tcaggacgcg gcgggcagcc cgggatatac acatcaaccg ggatgaattt
    51661 atcgacgccc tgcacaacgg aataaatatc gtacatacca ccagagttgg cacaggcccc
    51721 cattgagata acccattttg gttccagcat ctggtcatac agacgctgaa taaccggtgc
    51781 cattttggta aagcaggttc ctgcaaccac catcaggtca gcctgacgcg gcgaagcacg
    51841 caatacttct gcgccaaaac gcgccacgtc atgcaccgcg gtaaacgaag tcaccatctc
    51901 aacgtaacag caggaaagac cgaagttata cggccaaatt gagtttttac gaccccagtt
    51961 aaccatgtca ttgagcttgc ccataaacac gtttttgtta acttcttgct ccagagggtc
    52021 ggttacgatc tcctgctttt gcagggggta acggtcgttc tcaccgttgg gatctatgcg
    52081 ggtgagcgta taatccatct taatgcctcg cggttagcgt tgacgattag cgatactgtt
    52141 cgtttccggg ttcatacact cgcggcgtga acgcgcgggc gtccagtcca gcgcgccaat
    52201 acgcaccaga taaaccagac ctgccagtaa cacaaaaata aaaattgcag cttccacaaa
    52261 gcctacccag ccgctttcgc ggatagaggt tgaccatgcg aacagataca gcgcttcaac
    52321 gtcgaagata acgaagaaca tggccaccag ataaaacttg gcggacaggc gtaagcgggc
    52381 ggagccgacc gaatcgatac cggattcaaa cggcacgttt ttcgacctcg cgcgtgcgcg
    52441 accgcctaaa aaccaaccgc ctaccagcat caggcaacac aggccaatgg caacgataag
    52501 aaagatagcg aatgcccagt gatgagcgat gacttcagtg gatgttgaca tactcattgc
    52561 ttactcatca aaagtagcgc cagattcact gctcttcacg gcagatggac gccacatcga
    52621 ttcatgggga ggaataaaaa aaaccttaca atcactgtag aaattctttt atacagctaa
    52681 ttgatgtggt cttttactcc tttctataac cttttgtcaa ctttaacaaa agtttcttca
    52741 cattagttta cataatatca acaccattag catttaatgc cctttcaccc cagatccttg
    52801 acgactccag gataattaga tgttgttgaa tcgtgtccgt tgtgaagcaa tggaaaaaat
    52861 acgggtctat tttgacagga atttgtgtcg attcctcccc ccaaaagaga gtattttctt
    52921 gatctgtgac acgcttttgt tattccataa caaaaacgca gcaacaaatt tacgtatttt
    52981 ttaacatcat tgtagcaggt gatttttttc aggcgattat ttgtgcgttc gggacgtgaa
    53041 tctctggtga ttgaaaaata aacagttttg tacgttctgc actatgcgag gaaggatttt
    53101 tactaaaaaa aagccgctgg ggtttaaaac acccccagcg gctcgttttt tacactattg
    53161 tctcaggaat tatctatcgt ccgtcgatta ctcgatatcc ctttcaatca acaatgaatc
    53221 atccccttcc ggagcagaca ttgggctgta ttgccacgga ttatggtagc tctccatcgc
    53281 ctgatagatc acctgggcca gctcattatt actggacggg tcatagcaca gcaaatattc
    53341 ggtatcaggt agcggcggta agccatctac tccactcagc acacgcaggt ccgggctcat
    53401 catctcaacc ggccttgccg tcacgccaag accggctttc actgccgcac gaacggccgg
    53461 aagcgtcgag gcgacataag ccagtcgcca tggaatatct gctttattaa gcgtcgccag
    53521 caccatatcg cgaaacggac ttggatcatc cagcaataca agagggatcg gctcgccttt
    53581 ttgcaatacg tattccgctg cgcagtacca gtgtgttggc gaggtacgca ggttcaatgc
    53641 cttaaacgct gaaggacgat gggtggttac catcaaatcc acttcctggg attccagcat
    53701 ttctgccata taggcattac gtttaacgcg gacatccagc gctaatttcg gataaaccga
    53761 actcacgcga tttaacagga aaggtaagat cgtatcggca gattcatctg aagcaccgat
    53821 agttaacacg ccctgaagat tactgaacat taatgatgag caggcctcat cattaaaacg
    53881 caggattttc ctggcgtaac caagaagttg aatgccatgt tcagttaaca gtttgttgcg
    53941 accgtgacga gcgaacagtt ctttcccaac gagttgttcc agacgctgca tttgctgact
    54001 tacggcggac tgagtacgac acacagcggc agctgcggca gcaaaagtgt tcagatcggc
    54061 aacagcaaca aatgttctca gcagatcgag gtcgaggtta attatcggac gatttgcact
    54121 tatcatatat tatcacttac tggcggctca tactgagctg gttaatgctg tgcacacaca
    54181 aacaagcaat tccatttgta atgtgcctcc ctggcagttt catccggaac ccggacgaaa
    54241 gtaaaaatgc atatgagttg cactaaaaaa gcgactcaca ttgttccgtt ataatgcctg
    54301 aagtagatca cagaatatat cttcagggat cgcatatcta ttaagttact cactcttttc
    54361 tatttatgac atgcgcgtgt ttgtataaat gtaaatgtga gtccttgttc cactctcgtg
    54421 cagcatcgct ggtcatacgc gaacacgtac aaacagcaat ggtgaagcgg catcaagagc
    54481 agggatccgt catttatgcg agcattttac cccaaaacct tttatttata agggtcattg
    54541 cgaattatct gatgcaaagt tatgttatgt tatgttatgt tatgttatgt tatgttatgt
    54601 tatgttatgt tatgttatgt tatgttatgt taggcaaagt aatcttctat tattaataag
    54661 cacatcaaaa cctttttgaa tattaaataa taattaatta gcatcatcct cattcattaa
    54721 ttccgcttaa caatagtttc acaccttatc cccattgcga cacctcggaa atcatcacgc
    54781 tgttaaactc tccgtaatgc ggaaatatca tcatcgcgtt tcattagtga attcttaaat
    54841 gaggcatttt cacacaatta tcttacagat aaaaaaacca gacttgcaat taagaattag
    54901 aacaattcac tatataacat tacatgtaaa gcatatacac ctcattattt tgtcattatt
    54961 aagttattaa cagcacaatc gagccttccc ctctggcaaa atcttattct gcagaccttc
    55021 aaaacaccgt cctgggggag tacattgttc taagctgact tccacggcag ggagtggcga
    55081 taacagcaaa aaaggtcaag attcatgtcc cccattgaaa aatccagcaa attagagaat
    55141 gtctgttatg acatccgtgg tccggtgctg aaagaagcaa aacgcctgga agaagaaggt
    55201 aacaaggtac tgaaactgaa catcggcaac ccagccccat tcggttttga cgcgccagat
    55261 gaaatcctcg ttgacgtgat acgcaacctg cctaccgctc aagggtattg cgattccaaa
    55321 gggctttact ccgcgcgtaa agccatcatg cagcactacc aggctcgtgg catgcgtgat
    55381 gttaccgtgg aagatattta catcggcaat ggtgtatcgg agcttatcgt tcaggcaatg
    55441 caggcattgc tgaacagcgg ggacgaaatg ttggttcctg caccagatta cccactctgg
    55501 accgcggcgg tttcgctttc cagcggtaaa gcggtgcatt atctttgcga tgaatcctct
    55561 gactggttcc cggacctcga tgatattcgc gctaaaatta cgcctcgtac gcgtgggatt
    55621 gttattatca acccaaataa cccaaccggc gcggtatatt ccaaagagct tttaatggag
    55681 attgtggaga ttgcacgtca gcataatctc attatcttcg ccgatgaaat ttatgacaaa
    55741 attctctacg acgatgctga gcatcactca attgcgccgc tggcacctga cctgctgacc
    55801 attaccttta acggactgtc gaaaacgtac cgcgttgcag gcttccgtca ggggtggatg
    55861 gtgttgaacg ggccgaaaaa acacgccaaa ggctacatcg aaggtctgga aatgctggct
    55921 tcaatgcgcc tgtgtgctaa cgttcctgcg caacacgcca ttcagaccgc gctgggtggt
    55981 tatcagagca tcagtgaatt tattacccct ggcggtcgtc tttatgagca gcgtaaccgc
    56041 gcgtgggaac tgatcaacga tattccgggc gtttcctgcg tgaaacctcg tggtgcgctg
    56101 tatatgttcc cgaaaatcga cgccaaacgc tttaacattc atgacgatca gaaaatggtg
    56161 ctggatttcc tgttgcagga aaaagttctg ttggtgcaag ggacggcatt caactggccg
    56221 tggccggatc acttccgcat tgtcacgcta ccgcgtgtcg atgatatcga gctgtctttg
    56281 agcaagttcg cgcgtttcct ttctggttat catcagctgt aatcttaatt tcactgccgg
    56341 agattgcatc cggcagtgtt atcccgccac aatgacctga tgatgtcatc atacgtaagg
    56401 tcactatgaa acagagccat ttttttgccc atctctcccg cctgaaactc attaaccgct
    56461 ggccgctaat gcgcaacgtg cggacggaaa atgtgtccga acacagtttg caggtagcga
    56521 tggtcgccca tgcgctggca gctatcaaaa atcggaaatt tggcggtaat gtcaacgccg
    56581 aacgtatcgc tttactggcg atgtaccacg atgcctcaga agtgctcacc ggcgatctcc
    56641 ctactccggt gaaatacttc aatgcgcaaa tcgctcagga gtacaaggct attgaaaaaa
    56701 tcgctcagca gaaactggtc gatatggttc cggaagaact gcgggatatc tttgcgccgt
    56761 taattgacga gcatgcatat agcgatgaag aaaaatcgct ggtgaaacag gcagatgcac
    56821 tgtgtgcata tctgaaatgt ctggaagaac tcgcggccgg aaataatgaa ttcttgctgg
    56881 caaaaacgcg actggaagcg acgcttgaag cgcgtcgcag ccaggagatg gactacttca
    56941 tggaagtatt tgttcccagc ttccatcttt cgctcgatga gattagccag gattcaccgc
    57001 tgtaagcagc cggagtctgc gtcgcatcag gcaataagcg ccggatgcga catcaggctc
    57061 ttgtcaaaac ggaaacagca tcgggatcat caccacacaa accgccatca cgataatggt
    57121 gaacggtacc cccaacttca caaagtcact gaagctgtaa tttcccggac ctaaaaccag
    57181 ggtgttaaca ggtgaagaca ccggtgtcat aaaggcggcg gatgctgcca tcgccacgac
    57241 catcgcaaat ggataaggcg acactcccat cgttttggca gcagccagcg caatcggagc
    57301 catcaacacc gccgtcgcgg tattagagat aaacagccca ataaccgccg acaagacaaa
    57361 caaacacccc agcatcatat gtggcccata accgccgcca atatccatca gccctttcac
    57421 cgccagcgcg acacctcccg ttttctgtaa tgccacagca aacggcatca tcccaacgat
    57481 caaaataatg ctcggccagt gaatggattt ataggcgctt tcagcatcta tacagcggaa
    57541 tttccccatc agcaggcagg cgatgatagc ggcaacagga ttaggaattt catttgtcag
    57601 cattaacgcc accattagca ccagacagaa aatggcatgg ggtgcctggc tgtgcgcggg
    57661 cgatgcttca ctcacctctt ccggtaagtt cagcgctacg aagtcgcggc cctgtttggc
    57721 cagcataccg atcagtttcc agttacccac aaccaggatg atatcgccca gcagcagagg
    57781 ctcatccgcc agcgaacctt ccagcgccac gccattgcgc ttgagcccca ccacattcag
    57841 tccgtagcgg gtacgaaaac caatttcgcg caccgattta ccaatcagtt ctgactcagg
    57901 aattaatgaa atctctgcca tgcccacatc aagggcctgg tcagaaaaat actcgccgcg
    57961 cagtaccatc ggctccagca attgctcact acaaaattgc cggagatcga catcagccgc
    58021 agacatatca ataagcaaaa cgtcacgcgc gcgaaattca gaaaccccat taacgttcac
    58081 gataacgcga cgaaaacgcc gccagcgttc aacaccgatg acgttagcgc cataacgctc
    58141 acgtaatttg agatcatcca gccgttgacc tatcattggc gatccggggc gaatagccag
    58201 acgtcgcgca cgcccggtca gtcgatattc acggataaga tcgcgaaagg ttcgacgcgt
    58261 ccagccttcg cgctgcgggg tctgggtatc ccctttcagc atgaaacgca tcactaacat
    58321 atacaaaata cccagcacca gcacgaccag gccaataggt gttacgctaa agaaactgaa
    58381 gccgtgatag ccttcacgca gcaattcact gttgactacc aggttcggcg gcgtcgccac
    58441 cagcgtcatc atgccgctaa tcagcccggc aaaacttaac ggcatcatca gacgtgacgg
    58501 cgaagtttgc atacgcatgg caacgcttaa caccacggga ataaagatag cgacaacgct
    58561 ggttgaactc ataaacgcgc caagccccgc gacggtcagc atcaacaaaa ccaacatttt
    58621 gatttcactg ttgcccgcca ctttgaccag ccatgttccc attacggtgg caacaccggt
    58681 acggaccaaa ccatcgccaa taataaacaa ggcggcaatc aggacaacgt taggatcaga
    58741 aaagccggaa aatacttcgg ggaccgtcag cgttccgctt aatgcaaacg cgacaataac
    58801 aaacaaagcg accgcatcca tacgcactct gcccgtcgca aacaagacga tggcaacggc
    58861 cagtaatgaa agaacccaaa tcaattcacc gttcacaact tatccttgtt aattgagggg
    58921 gatgacttga ttctgccata aaaaagcccc gacgagacgg ggctaaatca tgatcaggtg
    58981 tttcactgaa taataacatc gccatttggc tgtttggtca cagtaatttg ctccagactg
    59041 tggaggacca aatcgacctc attcaggcgc ggggtatctg ccggagcgtt aaccgcaatg
    59101 acatggcaac ccgccgctag gccagaaagc acgccagcgg gagcatcttc caccaccaca
    59161 cactcctgcg gcgcaagccc cagcagctgc gcacctaaca gatacgcatc aggttctggt
    59221 tttccgcgct tcactcgctc agcggttaca aacacctctg gtgcgggaag ccccgctatt
    59281 ttatggcgcg ctcgcgctac cggcatggag ccagaagtca caatggccca cggaatacct
    59341 gctttattca aatgattgag taaggcgatt gccccagaaa gcgcggtaat accttcggtt
    59401 tccgtggcct cgatttgctc cagacgcgta aactcggcgg caatatcagc ctcggatttg
    59461 cccgccataa aatggcgcaa agaggtgatc gcctgtttac cgtgaatgaa agccagcacc
    59521 tcttccggcg ctaacccatg acgtctggcc cagttgctcc acgcccgttc taccgcaggc
    59581 agggaatcca ccagcgttcc atcaagatca aacagaaaac ctttgcaccg cacgcgggcc
    59641 tcctcaggcg ttgataattt gattaatttc gttggcgctc aaatggtact gacgcgggca
    59701 ggcatgccac acattaagca tgcgctgata tttttcccac attggcgtct gggcgttaaa
    59761 accatgagtc ccagcgtcaa aatgggtata gcgcccttcc acattaacca taaagcggac
    59821 ataaccgagg taacgtgctt cagtggcggc gtcaaagccg aggaaggtga cacgacgttc
    59881 atcgatggat tgctggtcct gcaaattaga ccaggaaaca tgcaacgcat gatacatctc
    59941 cataatgtcg atgatagtgc ggcaggtttc ttctttcagc tcgccaaact cgcgatccaa
    60001 ttcacgcatc tgtaatccgt aaccacgctc aataattgtt tgcaggcgac ggtaacgttc
    60061 agcatttgcc ggatcgagca tagtcatcat cttgtactga ttagacaaaa taagacgttg
    60121 cgcgttggtc atttccattg ttgactcctg tatcactcta ctacggtgaa aaaaaagaag
    60181 gctgagtatg ccttctttta tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag
    60241 ttttatccag ttgtttgaag gcgcgcttaa gcgtgtcagc taatgcctgg taatcaggct
    60301 tgccttcaac gggtgccaac acctgtccag actcctgcaa tttaccgcga acttcataaa
    60361 accagttaag gattgcaggg ggtaatggcg ttacagaacg cttgcccagc caccacaatc
    60421 cctgcatggg taaacttaag gcgaacagtg cagtggcaac tgccggccca agcagaccgc
    60481 ccagggcaat ctgccagcag agagtaaata cggcgatcgg cggcataaaa cggatcgcat
    60541 aacgcgtcat cttgataacg cgattttcga caaagaccgg ggcaaggcgt ttttccagtg
    60601 gccacgtctt tgagtaatgc tgtccgcggc gaaacaagct aaaaaaatta acagaacgat
    60661 tatccggcgt tgacatgctt cacctcaact tcacatataa agattcaaaa atttgtgcaa
    60721 attcacaact cagcgggaca acgttcaaaa cattttgtct tccataccca ctatcaggta
    60781 tcctttagca gcctgaaggc ctaaatagta catattcatt gagtcgtcaa attcatatac
    60841 attatgccat tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt tcttccatca
    60901 tgcaaaaaaa atttgcagtg catgatgtta atcataaatg tcggtgtcat catgcgctac
    60961 gctctatggc tccctgacgt ttttttagcc acgtatcaat tataggtact tccatgtcga
    61021 gtaagttagt actggttctg aactgcggta gttcttcact gaaatttgcc atcatcgatg
    61081 cagtaaatgg tgaagagtac ctttctggtt tagccgaatg tttccacctg cccgaagcac
    61141 gtatcaaatg gaaaatggac ggcaataaac aggaagcggc tttaggtgca ggcgccgctc
    61201 acagcgaagc gctcaacttt atcgttaata ctattctggc acaaaaacca gaactgtctg
    61261 cgcagctgac tgctatcggt caccgtatcg tacacggcgg cgaaaagtat accagctccg
    61321 tagtgatcga tgagtctgtt attcagggta tcaaagatgc agcttctttt gcaccgctgc
    61381 acaacccggc tcacctgatc ggtatcgaag aagctctgaa atctttccca cagctgaaag
    61441 acaaaaacgt tgctgtattt gacaccgcgt tccaccagac tatgccggaa gagtcttacc
    61501 tctacgccct gccgtacaac ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg
    61561 gcaccagcca cttctatgta acccaggaag cggcaaaaat gctgaacaaa ccggtagaag
    61621 aactgaacat catcacctgc cacctgggca acggtggttc cgtttctgct atccgcaacg
    61681 gtaaatgcgt tgacacctct atgggtctga ccccgctgga aggtctggtc atgggtaccc
    61741 gttctggtga tatcgatccg gcgatcatct tccacctgca cgacaccctg ggcatgagcg
    61801 ttgacgcaat caacaaactg ctgaccaaag agtctggcct gctgggtctg accgaagtga
    61861 ccagcgactg ccgctatgtt gaagacaact acgcgacgaa agaagacgcg aagcgcgcaa
    61921 tggacgttta ctgccaccgc ctggcgaaat acatcggtgc ctacactgcg ctgatggatg
    61981 gtcgtctgga cgctgttgta ttcactggtg gtatcggtga aaatgccgcg atggttcgtg
    62041 aactgtctct gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa cgcaacctgg
    62101 ctgcacgttt cggcaaatct ggtttcatca acaaagaagg tacccgtcct gcggtggtta
    62161 tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag ccgcctgact gcctgatttc
    62221 acaccgccag ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag
    62281 aggataaacc gtgtcccgta ttattatgct gatccctacc ggaaccagcg tcggtctgac
    62341 cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt
    62401 caaacctatc gctcagccgc gtaccggtgg cgatgcgccc gatcagacta cgactatcgt
    62461 gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg aaaatgagct acgttgaagg
    62521 tctgctttcc agcaatcaga aagatgtgct gatggaagag atcgtcgcaa actaccacgc
    62581 taacaccaaa gacgctgaag tcgttctggt tgaaggtctg gtcccgacac gtaagcacca
    62641 gtttgcccag tctctgaact acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt
    62701 tatgtctcag ggcactgaca ccccggaaca gctgaaagag cgtatcgaac tgacccgcaa
    62761 cagcttcggt ggtgccaaaa acaccaacat caccggcgtt atcgttaaca aactgaacgc
    62821 accggttgat gaacagggtc gtactcgccc ggatctgtcc gagattttcg acgactcttc
    62881 caaagctaaa gtaaacaatg ttgatccggc gaagctgcaa gaatccagcc cgctgccggt
    62941 tctcggcgct gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg atatggctcg
    63001 ccacctgaat gcgaccatca tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt
    63061 cactttctgc gcacgcagca ttccgcacat gctggagcac ttccgtgccg gttctctgct
    63121 ggtgacttcc gcagaccgtc ctgacgtgct ggtggccgct tgcctggcag ccatgaacgg
    63181 cgtagaaatc ggtgccctgc tgctgactgg cggctacgaa atggacgcgc gcatttctaa
    63241 actgtgcgaa cgtgctttcg ctaccggcct gccggtattt atggtgaaca ccaacacctg
    63301 gcagacctct ctgagcctgc agagcttcaa cctggaagtt ccggttgacg atcacgaacg
    63361 tatcgagaaa gttcaggaat acgttgctaa ctacatcaac gctgactgga tcgaatctct
    63421 gactgccact tctgagcgca gccgtcgtct gtctccgcca gcgttccgtt atcagctgac
    63481 tgaactggcg cgcaaagcgg gcaaacgtat cgttctgccg gaaggtgacg aaccgcgtac
    63541 cgttaaagca gccgctatct gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa
    63601 tccggcagag atcaaccgtg ttgcagcgtc tcagggtgta gaactaggtg caggcattga
    63661 aatcgttgat ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg agctgcgtaa
    63721 gaacaaaggc atgaccgaaa ccgttgcccg cgaacagctg gaagacaacg tggtgctcgg
    63781 tacgctgatg ctggaacagg atgaagttga tggtctggtt tccggtgctg ttcacactac
    63841 cgcaaacacc atccgtccgc cgctgcagct gatcaaaact gcaccgggca gctccctggt
    63901 atcttccgtg ttcttcatgc tgctgccgga acaggtttac gtttacggtg actgtgcgat
    63961 caacccggat ccgaccgctg aacagctggc agaaatcgcg attcagtccg ctgattccgc
    64021 tgcggccttc ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg gtacttctgg
    64081 tgcaggtagc gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc
    64141 tgacctgatg atcgacggtc cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa
    64201 atccaaagcg ccgaactctc cggttgcagg tcgcgctacc gtgttcatct tcccggatct
    64261 gaacaccggt aacaccacct acaaagcggt acagcgttct gccgacctga tctccatcgg
    64321 gccgatgctg cagggtatgc gcaaaccggt taacgacctg tcccgtggcg cactggttga
    64381 cgatatcgtc tacaccatcg cgctgactgc gattcagtct gcacagcagc agtaatctcg
    64441 tcatcatcca cagctttgcg ctgcggatat ctgaaccgga aataatcact atttccggtt
    64501 ttttattctc ttaatttgca ttaatccttt ctgattatct tgcttaactg cgctgcatca
    64561 atgaattgcg ccatctcact ttgcatactt accactttgt tttgtgcaag ggaatatttg
    64621 cgctatgtcc gcaatcactg aatccaaacc aacaagaaga tgggcaatgc ccgatacgtt
    64681 ggtgattatc ttttttgttg ctattttaac cagtcttgcc acctgggtag ttccggtggg
    64741 gatgtttgac agtcaggaag tgcagtatca ggttgatggt caaacaaaaa cacgcaaagt
    64801 cgtagatcca cactcatttc gcattctgac taacgaagca ggcgaacctg agtatcaccg
    64861 cgtacagctg ttcacgacgg gcgatgaacg cccgggcctg atgaacttcc cgtttgaagg
    64921 gttaacctca ggatcgaaat acgggacagc cgttggcatc atcatgttta tgctggtgat
    64981 tggcggcgcg tttggcattg tgatgcgtac aggaaccatt gataacggta tcctggcgct
    65041 tattcgccat acccgcggga atgaaattct ctttattcct gcgctgttta ttctgttttc
    65101 acttggcggc gcggtatttg gtatgggaga agaggccgtc gcctttgcca ttatcatcgc
    65161 accgctaatg gtccggctgg gctatgacag tattaccacc gtcctggtga cttatattgc
    65221 cacgcaaatc ggttttgcca gttcgtggat gaacccgttt tgtgtggttg ttgctcaggg
    65281 gattgccggc gttccggtgc tttctggctc cgggttgcgc atcgtggtgt gggttatcgc
    65341 cactctgatt ggcctgatct ttaccatggt gtacgcctca cgagtgaaaa agaatcctct
    65401 tctgtcacgc gtgcatgagt ccgaccgctt ctttcgtgaa aagcaggcgg atgttgaaca
    65461 acgtccgttt acctttggtg actggctggt attgattgtc ctgacagccg taatggtctg
    65521 ggtgatttgg ggcgtgatcg ttaatgcctg gtttattcca gaaattgcca gccagttctt
    65581 caccatgggt ctggtgattg gcatcatcgg cgttgttttc cgccttaacg gcatgacggt
    65641 taataccatg gcttcatcct ttaccgaagg ggcgcgaatg atgatcgccc ctgccctgct
    65701 ggtgggtttc gccaaaggga ttttgctgct ggttggtaat ggtgaagcag gtaatgccag
    65761 cgtgttaaat actatcctca acagcattgc caatgccatt agcggtctgg acaacgcggt
    65821 cgcggcctgg tttatgttgc tcttccaggc tgtatttaat ttcttcgtga cgtccggttc
    65881 tggtcaggcg gcattaacta tgccgttact ggcaccgctt ggcgatctgg tcggtgttaa
    65941 ccgtcaggtt accgtgctgg cgttccagtt tggtgatggc ttcagccaca tcatttaccc
    66001 aacctcagct tcgttaatgg caacgctcgg tgtgtgccgg gtggacttcc gtaactggct
    66061 gaaggtgggc gcgaccctgc ttggactgct gtttattatg tccagcgtcg tagtgatcgg
    66121 cgctcagttg atgggctacc actaaaaatg ttaagagccg caattgcggc tcttttttca
    66181 ttctgccgtt tcagtctcta cggcttcatt tttggcattg cgcttcatcc acaacgccag
    66241 cgctttcagc gagtctggag tgaactcatc gcagcgtgcg gtgatttctt ccggcgtcag
    66301 ccagcaaact tcactgactt catcttcctg tagggcgaag ggaccgtgag agacgcaact
    66361 gaacaacgcg ccccagacac ggcaattttt atcttcgaaa tagaactgcc cgtgctcggc
    66421 aaaggggaca ccggcaatgc ccaactcttc ttccgcttcg cgacgcgcgg attccagcag
    66481 ttgctcatcg gcctgaacta caccgcctgc ggtcgcatct aacatgccgg gtaaaaagtc
    66541 ttttgtctcg gtacgacgct ggaccagaat tttgcccatg ccatcatgca cgacgatgta
    66601 agttgcacga tgacgcagac actgtgcccg catttgttcc cggctggctt gtgcaatgac
    66661 ttcgttctct tcattgacaa tatccaccca ttcagtactt gccaaacgac gctgttccat
    66721 tatcaggaaa ccttcttttt ctggcgctct tacggcgcat tttgagttgt gggtaaatta
    66781 cggattaatc gcgacctgcg caatgatact ttgatcattg agtgcgatta cactaagtac
    66841 gtcattatcc agcatgccat aactcgccgg attaccgcct ttcggaatac tcaccgagcc
    66901 ggggttgaag tggaaaatct ccccccgctg ttctgccact ggcagatggg tatgaccgta
    66961 caccagcaca tcgttctggt ttaaagcagg tagattttcc gggccaaaaa gatgaccatg
    67021 cgtcaaaaac agacgttgtt tttccagtaa tacctgttgc cacggcgcgg ttatcgggaa
    67081 atgcagcatc atttgatcca cttcgctgtc gcagttgcca cgcacagcga taaccttatg
    67141 tgccacttca ttaagccgtt cagcgacttt ggctggcgcg taaccctccg gtaaagcatt
    67201 acgcgggccg tgattcaaca cgtcgccaag gatcaccagc cactgggcac cgctttggac
    67261 aaacaactcc acaacacgtt ccgtcgccgg taacgaccca tgaatgtccg atgcaaacat
    67321 cagtttcatc actcactcct cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt
    67381 gctatcagcc agattgcttc gccgaaagtg caataaaacg ctggacagac gctcgctgcc
    67441 actggaatgt cgcataatcc accaggcgtt ctggcacctc atcgccatgt agtaccaggc
    67501 ggttaatcat tagcgccaga tcagtatcag caatgcacca ttcaccaaat aaattcggct
    67561 gacccagtgc taacaaatgt tctgccatcg cgaacagttt ctctgcactg gcttttccct
    67621 cggccgttag tggcgctttt ttcgcccccg caaagacaac atccgtcgga cgctcttcgc
    67681 ggatgggcat cagatcgctg cgcagccagg cctgaatctg tcgtgcacgc gcacgatttt
    67741 ctaaatcaag cggataaata cgttcccagg tcggtggcgc aaatcgatct tccagatact
    67801 ccgcaatggc agaagattca ctcaactcaa aatcatcgat ttgtaataac ggcacacggc
    67861 gtgtctgacc gtaaccttgc cacgtcggct gcaaatgttc accgctgtcg aggtcgatgg
    67921 tcttgatatg aaacgacagg cctttttcct gcaacgccac ccaggcggat aacacataag
    67981 gggagaaaaa gtgggcatct gaccaaagcg tgatagcggg tttactcatt atgtcctcgg
    68041 caataaaggg ggctggctat caaactatag agcctttttg tcactgtcac gtgacgaata
    68101 ctcacgtaag tcgccgcgct ttctatactt gtggtgttca gatgattaaa caaccggagc
    68161 tgcaatgatc gatctctatt tcgccccgac acccaatggt cacaaaatta cgctgtttct
    68221 cgaagaagca gagcttgatt atcgcttgat taaggtagac ctggggaaag gcggtcagtt
    68281 tcgtccggaa tttttgcgca tttcgcccaa caacaaaatt ccggcaattg ttgatcattc
    68341 tcctgccgat ggcggcgaac cgttaagcct ctttgagtct ggtgccattt tgttgtatct
    68401 ggctgagaaa acaggactct ttttgagtca tgaaacgcgt gagcgcgccg ccacattaca
    68461 gtggttattc tggcaggtag gcggactggg gccgatgctt gggcaaaatc atcattttaa
    68521 tcacgcagcc ccccaaacca ttccttacgc tattgaacgt tatcaggttg aaactcagcg
    68581 tctttaccat gtactaaaca agcggctgga aaactcgccc tggctgggag gcgagaacta
    68641 cagcattgcg gatattgcct gctggccgtg ggttaatgcc tggactcgcc tgcgaattga
    68701 cctcgcaatg tatccggcag tcaagaactg gcatgagcgg atccgttcgc gccctgccac
    68761 cgggcaggca ctgctaaaag cacaactcgg tgatgagcgt tcggatagtt aacagaaaca
    68821 ggttctcgtg tattatttca tcctaagtaa aacaacggag aacctgcaat ggcacaacct
    68881 gccgctatta ttcgtataaa gaaccttcgt ttgcgtacgt ttatcggaat taaggaagaa
    68941 gaaattaaca accgtcagga tattgttatc aatgtgacga tccactaccc cgccgataaa
    69001 gcgcgcacta gcgaagatat caacgatgcg ctgaattatc gcaccgtaac gaaaaacatt
    69061 attcagcatg tagagaataa ccgtttctct ttgctggaaa aattaactca ggatgtgctc
    69121 gatatcgcac gtgaacatca ctgggtgacg tatgctgaag tggagatcga taaactgcac
    69181 gcgctgcgct acgccgattc ggtatccatg accttaagct ggcagcgtta atcgccatat
    69241 cgggaggctg catgaacata gtgatcaccg gagggacggg attaattggt cgccatttga
    69301 ttccgcgttt gctggagctg ggccatcaaa ttacggtagt gacgcgtaac ccgcagaaag
    69361 ccagttccgt tctcggccct cgggtgacac tatggcaagg gcttgccgat caaagcaacc
    69421 tcaacggcgt tgatgcggta atcaacctgg ccggagaacc gattgctgat aaacgctgga
    69481 ctcacaagca aaaagagcgt ctctgccaaa gccactggaa tatcacgcaa aaactggtcg
    69541 atttgattaa tgccagcgac acgccgccgt cggtactcat ttccggctcg gcaacgggct
    69601 attatggcga cttaggtgaa gtggtggtta ccgaagagga accgccgcat aacgaattta
    69661 ctcataaact ctgcgcccgc tgggaagaga ttgcctgccg ggcgcaaagt gacaaaacgc
    69721 gagtgtgcct gctgcgaacc ggcgtggtac tggcaccgga cggcggtatt ctcggtaaaa
    69781 tgctgccacc gtttcgtctt ggcctgggcg ggccgattgg ttccggtcgg cagtatctgg
    69841 cctggattca tattgatgat atggtcaacg gcattctctg gctgctggat aacgagctgc
    69901 gcgggccatt taatatggtt tcgccctacc cggtacgcaa tgaacaattt gcccatgcgc
    69961 tcggtcatgc gctgcatcgc ccggctattt tgcgcgtccc cacaaccgcc attcggctgt
    70021 taatgggcga atcttcagta ctggtattag gtggacaacg cgcgctgcct aaacggctgg
    70081 aagaagcggg ttttgcgttt cgctggtacg atttagaaga ggcgctggcg gatgtcgttc
    70141 gctgatgtgg tttacagcaa acatccgcca gttaactccc tgtgttacag gattagtggc
    70201 tttgcgcgat aagatcgtct ggtgaaagtc gggtcaccat cataactaac tctctgtcta
    70261 aacctctatc cagcatctcc tgagcaatac gcagggcttc ttcgtgtttg ccctgcattg
    70321 cgccttcttc acgtaatctg tcagcaatgg tcatcagttt ctccttttct tgtggtgcgc
    70381 gttctgttat ctcaccaata aatgcacgga aacgctgggc atcccctgtt tgtaatacgt
    70441 aattaaacag ggcttttagc tgtctgtcat tagtgttccc tgtaactagc agcgaaacaa
    70501 tttggtcgac taatcccaac agatcgcgct gacgaatatg tttctgaatt aactccaaca
    70561 gcgccatttt gcggtgttgc ataatctcgt catccggcac cacggcaata tccaccaacg
    70621 gaaacgctga tgaatatatt ttgcgggcta tagcaggctc ggcaaattca tcaagccagc
    70681 agagtgaata aggataagga cttctgcaac catgataaaa cagcatcggg atcaccaatg
    70741 gaagctcttt atagcccgca tcaagatggt tttgcattgc cgcaatggaa taacgcatca
    70801 tgcgaaaagc cattaattct tccggcttac tttggtgctc tatcactaca taaatataac
    70861 ccactccctc ctgcgttttc acagaccaca agaggtcgga ataatattgc cgcaggtctt
    70921 catcaataaa actgtttggt tctagtttaa gcgtcgttaa atcacacagt ttgcgcagcg
    70981 gcgcgggaag atgaatatta ataaaattcc gcgcggtgtc tggatggcgt aaaaaagatt
    71041 taaataccgc atcatgcggc gtggaagttg tcgatattgt catggcgttc cgtcacccct
    71101 caaatttaag atgacgcgac aataaccacg attaacaccc aatgcagcag gcagttttta
    71161 tctttggaca gcgaattcag aagtattttt agcggactgc aacttttaca tttcagcgga
    71221 agctgactcg caaattcccc ttgaaaatac tccggcagcg ttagccaccg gagaggaaat
    71281 tatttcagcg atcccttaag gaactgttgc agacgagggc tttgcgggtt gccaaataac
    71341 tgctccggcg cgccctcttc ttctattttc ccctgatgga ggaaaatgac gtgagtagaa
    71401 acatggcgag caaagcccat ttcgtgagtc actaccacca tggttttccc ctcttctgcc
    71461 agttgctgca taatacgtaa cacttcgcct accagttcag gatcgagcgc cgaggtaggt
    71521 tcatcaaaca gtaaaacttc tggttccatc gccagcgccc gcgcgataga aacacgctgt
    71581 tgctgaccgc cggaaagatg caccggatat ttcccctgcg cacgttcgtc tatcccgact
    71641 ttcgccagat acttcaccgc ccgctcgcac gcttcctgct tgctcaggcc caacacctga
    71701 atcggcgctt ccatgacgtt ttccaacacc gtcatatggc tccagagatt gaagtgctgg
    71761 aataccatcg tcaggcgtgt gcgcagtaag cgcagttgat ttttatcggc gactttgagt
    71821 tgaccgtctt tgtcgcgcac cagattgatc gtctggccat tgaccacgat cgatccttca
    71881 ctcggttttt cgaggaagtt aatgcagcgc agaaaggtac ttttccccga tcccgacgat
    71941 ccgatgatgc ttattacatc tccggcattc gcttgcagtg agaccccttt cagcacttcg
    72001 tgttcgccat agcgtttgtg caaatcgata acgtttaatt tattctcgga catcgtattc
    72061 tcagtgcgtt gaagaaggtt tcacatgctg caaccagcgt ttttccgctc tgcgaaagag
    72121 gctgatcagg acataagaga tgattaaata gagcaccgcg gcaatgccga aggcggtaaa
    72181 aggttgatac gtggcggcgt taatatcgcg ggctattttc agcagatccg gcacagtggc
    72241 cgtaaacgcc agcgcagtag agtgcagcat caggatcact tcgttgctgt atgccggtaa
    72301 cgcaatacgc agcgcagaag gcaaaataat gcagcgatac attttaaagg tcgagaagcc
    72361 ataggctctg gcggcttcaa tttccccatg cggaaccgaa cggattgccc cagcaaaaat
    72421 ctcggtagtg taagcgcagg tgttaagcgt cagcgccagc acggtacagt tcaggccact
    72481 gcggaagaaa gcgttaagga attcggttcc cttaacaatc tcaagcgtgt acatgccgga
    72541 atagaacacc agcaactgaa catacagcgg cgtaccgcga aaaatatagg taaataacca
    72601 gattggaaac tggatgtatt tattactgga gacacgacca atcgccagaa acagcgccag
    72661 gactccgcct atcactaccg acaaaataag cagccacaga gtgatcgcca caccagtaaa
    72721 gcgataaccg tcggtccaca gcagcggttt ccagtattca tgtaagattt cgatcacagg
    72781 tcagccctct tcacacccac ggagtagcgg cgctcaagga acagcagcac accattggaa
    72841 acggtggtga aaaccaggta aatcacgcca cagacgatgg cgaaatagaa cggttcccag
    72901 gtacttttgc ctgccagctg cgtggctttg accacatctt ccaggccgag taacgaaacc
    72961 agtgcggtag atttgaggat cacctgccag ttgttgccaa tgcctggcag ggcgtaacgc
    73021 atcatcgccg gaaacatgat cctccgaaac acttgcccgc gagtaaaacc gaacgccgtc
    73081 gccgcctcta tatgcccttt cggcactgcc ataaacgcgc cacggaacgt ttcggtaaaa
    73141 taagcaccgt agataaaacc gagagtgata ataccagcga ccatcggatc gatatcaatc
    73201 tgcccgacgc ccatcgcctc cgtcaccatg tttagcgcaa tctgcaaacc atagaaaatc
    73261 agcagcatca acaccaagtc cggcacgcct cgaatcagcg tggtgtaccc ttcgaaaatc
    73321 agccccaaaa gccgattttg cgagagctta ccgccagcgc caattaaacc gatgattaca
    73381 gcgagcacta cagagctgat agccagctcc agcgtgacga gcgcaccctg taaaataaca
    73441 cctgaaaacc catacaacat gctgcctgtc ctgtcgtgtg tggtgaatta ccgcctcttg
    73501 tctccctccg gttacccgaa gggagcgggc tttctttcac cgatggcgat taaccaccat
    73561 aaacatcaaa atcgaagtac tttttcgcta atttctcgta agtaccgtca gcgcgcattt
    73621 cggcaaaggc tttgttcagt gcctcgcgca gttcgttatc ttctttacgc aggcccatgc
    73681 cggtccctac gccaaacagt ttttcatctt taacagacgg accaccgaat ttgtaatctt
    73741 taccgacggg ttgtttgagg aaaccttcgc tggcagcgac ctcatcctgg aacgcggcat
    73801 caatacgtcc ggcagtcagg tcagaataaa tgttgtcctg cccctgatac gagacgattt
    73861 caatgccttt tggtgcccag tgttcattac cgaacgtctc ctgggtggtg ccctgcaata
    73921 cgcctacccg tttgcctttc agcgactcga ctgtcggctg aatgtcagaa tttttcgcca
    73981 ccaccagacg agaatcggca gcgtacagtt tgtcggtgaa ggcaatttct tgctgacgtt
    74041 tttccgtaat ggaaagcgat gacatgatgg cgtcaatctt cttcgctttt aaggacggga
    74101 ttaacgcatc cagcgggttt tcgacaaacg tacattgcgt attgatgcgc ttgcacaact
    74161 ccttcgccag ctcgatatcg aaaccaacca gttcgccttg tgaatttttt gattcaaatg
    74221 gcgcataggt cgggtcggta ccgatgcgga tgttttgcgg aattgcagca agtgccgcag
    74281 ttgcgctgga gaaggccaga accagagaga gcgatagcac cagttttttc ataatgtcct
    74341 caactgacag tctttttata gggaatattt acaggtttgt tacacttatc gtgccataaa
    74401 attgaccatc aaggcaaaat atcccgcccc gttagacgta ttatttttca ttaaaaatgc
    74461 ttaagtatgc atttcaatgc accacgatag tgcaacaaac ctggcgtgca ccatgatgat
    74521 cattccatca ggtacagctt cccagcgacg tatcagtcac cgtagacatt aaagtcgaaa
    74581 tactttttcg ccatcttgtc gtaggtgccg tcctgacgca gctcgccaag cgccttattg
    74641 aaggcagccg tcagttcagc atcatcttta cgtagcccta caccggtgcc atcaccgaag
    74701 tattttttgt cttttactga tgagccagca aaggcgaaat ctttaccagc aggttgcttg
    74761 aggaatcctt cgctggcagc aacttcatct tgtaacgcag catccagacg tcctgcagcc
    74821 agatcggaat agaccaaatc ctggttggca taggccacca catccacgcc tttactacgc
    74881 caggtctcgt tagcgtaagc ttcctgggtt gatccctgca gcacaccaac atgtttacct
    74941 ttcagtgaat ccagcgttgg ctgaatcggt gaacctttgg cagcaatcaa acgagaatct
    75001 gcggcgtaca gcttgtcgga gaaggcaatc tcctgctgac gtttatcggt aatggaaagc
    75061 gacgaaataa tagcgtcgat ttttttcgct ttcagtgagg ggagcagcgc gtcaaagtca
    75121 ctggcaaccc aggtacattt cacctgcatc cgtttgcaca tctcgttacc gagatcgata
    75181 tcaaagccaa caaaatcacc tttagcatct ttcgacgaga acggcgcata ggtggtatcg
    75241 gttccgatac gtaccgtctc cggtagcgcc gcatagctgg aagccgctgc ggagagaccg
    75301 actaataaag acagagcgag aatcgacttc ttcatacata accctcaagt gaaaatggct
    75361 tttttatgtt gtgtattgtg ttgtgtgttt gcaggctctt tcatgcaggt cttatgccat
    75421 cttgacgaca acagtaacat tcaacgttaa atatgttaat aagacgttgc attattgtcc
    75481 tgaagttgaa gatagcaggt atagcggttg aatcgcagcg tttcgattga atggcagaaa
    75541 caaaatgtcg aggatttgat cgcggttgca aaattgccct gaaacagggc aacagcggag
    75601 ttatgcgccc tgccagcggg caaagagatc ttcaggaagg gttattgcaa actggtcaag
    75661 aacacgatta accgtctgat ttatcacatc atcaagggat tgcgggcgat gataaaacgc
    75721 cggaacggga ggcataatca ccgcaccgat ttctgccgcc tgagtcatta aacgcagatg
    75781 gcctaagtgc aatggtgttt cacgcacgca gagcaccaac gggcgacgct ctttcagcac
    75841 cacatctgcc gcacgggtca gtaagccatc agtatagcta tggacaatgc cggacagggt
    75901 tttgattgaa cagggtaaaa tcaccatccc cagcgtctgg aaagaaccgg aagagatgct
    75961 ggcggaaata tcgcgcgcat cgagcgtgac atcagctaat gcctgcactt cgcgcaggga
    76021 aaaatccgtt tcgagggata aggtctggcg cgccgcctgg ctcatcacca gatgcgtttc
    76081 gatatctgtg acatcgcgca gaacctgtaa taagcgcacg ccataaatcg cgccgctggc
    76141 accgctgatg cctacaatga gtcgtttcat aaatagttgc cctgtcagac ttgcaggcag
    76201 actttgcagt atttcggcgg gagttgcaag tcagggtgcc agaccggcac cctcagcgaa
    76261 ggcatcatcc ttcgttatgc atttcgagat tttccacttc gttctgacgt tgcactgctt
    76321 tggcgtcatc attacgtaac gtatcgagga aatcgaggta gccctgatca acatctttgg
    76381 tgacgtagac gccgttgaac accgagcatt caaactgctg gatatccgga ttttcagcgc
    76441 gaacggcgtc gatcagatcg ttcagatcct ggaaaatcaa cccgtcagca ccgatgatct
    76501 ggcgaatttc atcaacttcg cgaccgtgag cgatcagttc cgtggcgctc ggcatatcaa
    76561 taccataaac gttcgggaag cgaatttccg gtgccgcaga agcgaggtac actttcttcg
    76621 ctccggcttc gcgtgccatc tcgataatct gctcagaagt ggtgccacgg acgatggagt
    76681 cgtcgaccag caggacgttt ttatcgcgga actcggcgcg gttggcattc agtttacggc
    76741 gcacggactt acgacgcagc tgctggcccg gcatgataaa ggtgcggcca acatagcggt
    76801 ttttaacgaa gccctggcgg tacggtttgc ccagaatacg agcaatttcc agcgcgatat
    76861 cacacgaggt ttctgggatc gggatcacca cgtcgatatc cagatcttcc cattcgcggg
    76921 caattttctc gcccagtttc gtgcccatat tcacacgcgc gctgtaaacg gaaattttgt
    76981 cgataaacga gtccgggcgg gcaaagtata catactcaaa caggcacgga ttgctgaccg
    77041 gattgtcagc acattgacgg gtaaacaact gcccttcttc agtgatgtaa atcgcttcgc
    77101 ccggcgcgac gtcacgcagg aaatcaaagc ccagcgtatc gagcgctacg ctttcggaag
    77161 cgaccatata ttctgtacgg ttctcgtcaa tatcacgttt tcccagtacc agcggacgaa
    77221 tcccgtttgg atcgcggaaa gcaaccatac cgtggccgat aatcatcgcc acacaggcat
    77281 acgcgccgcg gattaagcgg tttgtggcag caatggcagc gaaaatattg tcggcttcca
    77341 gcgggtagtg gcggaagttg tccagctcgc tggcgaagat attaagcaga atttccgagt
    77401 cggaagtggt gttgatgtgg cggcgttttt cttcaaacag ttttttacgc aactcgtgag
    77461 cgttggtcag attgccgttg tgggcaagcg taatgccata cggggagtta acgtaaaacg
    77521 gctgcgcttc agaggcgctg gagctgccag ccgtagggta acgcacatga ccaatgccca
    77581 tattgccctg caaacgctgc atatggcgag cttcaaatac atcgctcacc agcccgttcg
    77641 ctttacgcaa acggaagcag ttattggcat ctatggtgat gatgccggcg gcatcctgac
    77701 cgcgatgctg aagcaccgtt aaggcatcat aaatcgactg gttaaccggc ataacaccgg
    77761 cgataccgac aataccgcac atacgtcttt ttcctcgtta agccacatct cagagcactt
    77821 acgctctggg caagaaactt gacgagcttt gcagataatc aaaaaaccat ctgatgataa
    77881 aactgaattg cgggatcagc tgtgatttgc tccagtcttc acttttcgac accccggtaa
    77941 aggagtcgag aaagaagaga atggcggcaa caatcaacac accgcgcaac gcaccgaaac
    78001 agacgcccag cacccgatcg gtgcctgaca accccgtttt ctccaccaac tggcctatca
    78061 cgaagttcac gatagcacca acgatcaggg tagcgataaa cagtaccgcg atggcaatcc
    78121 catttcgaac cagttcgtct tcaaagcccg taaaccagac tgacaggtaa gtgtagtaat
    78181 gactggcaac aaagaaagca caaccccatg tcaccagcga taacgcttca cgaacaaagc
    78241 cgcggatcag gctaaccaga gaggaaaaag caatcaccgc gattatggcg taatcaatcc
    78301 agaccatatg tgtcccacga ttttacgccc tgtcatcctg ttcggggcgc attctaacag
    78361 aaaaagaaaa cgtttgcgta gggatttcct tcccgcgcat caataaaaat ggcgctgaaa
    78421 aaatattcaa cgccatcgac tttttatgcc tttgcggcat cgtgcaatgc gtgtcggatg
    78481 cggcgtaaac gccttatccg acctacggtt ctaacactgc ccaagcctga taagacgcgc
    78541 cagcgtcgca tcaggcaaga ctgtgttaat tcggcgtata gcccattacc acgccactta
    78601 agccagaaag ttgcttcaac tcacccagcg aacctttcag cttatctttc gaggcatccg
    78661 gcccaaccag aatacgggta attttaccct gcactggcgt ggatggcgac gtataaaccc
    78721 gataaccggc accgcgcagc ttaccgacaa tctcattcac tttatcggca tttttcagcg
    78781 cacccagttg cacaacatag gctttacccg ttggcgctgc tttttcttcc acgaccggct
    78841 tcggttctgg cttcggcgca ggtggtgctt caacctttgg tttaggcggc tccaccggtt
    78901 tcggcttcgg tggggcgacc ggtgcaggtt ccggttcaaa ctcggtgtta ttggctgcaa
    78961 tagtggccgg atcgagcgac ggtgctgcgg catcacctgc ccgcacctct tccgctgcgc
    79021 cttccggcgg ctgcgtcggc aacgcctggg tggcggctgg catcatatca ggctcatcac
    79081 gatcgcccgc tttcggcacc agcgggatag ccgcgaactc atcctgataa tgttttttct
    79141 gcccgtccag cagccctgga agtacaatca cccccagcgc caccagcacg atcgtgccca
    79201 ctaaccgatt ctgaaactta cttgccaccg cttctcctcg cgtcaatcac ttccatgaca
    79261 tgtgcgaccg tgtggaaaga accacacacc agcacggtgt cttccgcttt agcgtccgcc
    79321 attgcggcat cccatgcctg cgcaacgcta tcaaatgatt tgccgttacc caaatgctca
    79381 agcagttgtt ctgccgtggc accgcgcggc ccttccagtg gcgcacaata ccagtcatca
    79441 accacgcttt tcaaccaggc cagagttccg gcaatatctt tatcatgtag cataccgata
    79501 accgccagca cgcgcccgtt tttcggtagc gctttcatac gcccggtgag atattccgcc
    79561 gcatgtggat tatgcgcgac atcaaaaata acgcgtggcg actcgctcac aatctggaaa
    79621 cgtcccggca aaattgcgct ggcaatcccg tcgcgaatgg cattttcact gacttccagc
    79681 ccgctggcac gcagtgccgc cagcgccgtt gcggcattcg gttgcgggac aagcggcaac
    79741 ggcagatttt ccagcgtgcc gtgagcatcg ctaaacgccc agtcatgatc ggtgacggaa
    79801 tagttccact caacgccccg acgttgtaac agtgcacctt tttcctgcgc cacatcagca
    79861 atggtagaag gcatttccgg ctcaccgaca attgccggtt tttcgctgcg gaagatgcct
    79921 gctttctcgc gaccaatact ttcgcgatct ggacccagcc agtcggtatg atccagcgca
    79981 atactggtta ctaccgcggc atcggcatcg acaatattgg ttgcgtccag acgaccgccc
    80041 agccctactt ccagaatcac cacgtcaagt tgtgcctgct tgaacagcca caacgccgac
    80101 agcgtaccgt actcgaaata ggtcagggaa atatcaccgc gtgccgattc aatctccgca
    80161 aaagaggcgg tgtgggccga ttccggcaat tcccggccct gcacacgtac gcgctcggta
    80221 taacgcacca gatgaggcga actgtagacg cccactttgt accctgccgc catcagaatc
    80281 gactccagcg tacggcaggt ggtgcctttg ccattcgtac ccgcaacggt aaacacaaat
    80341 ggcgctggtt tcaggacgcc aagacgcgcc gcgacctggc tcacgcgctc aaggccgagc
    80401 tcgatagttt tactgtgcag gttttccaga taagaaagcc acgaagccag aggcgacgcg
    80461 gcttgaggag tgcgtttgat aatcatggta tccgctgatt cgttacggtg agaatagcaa
    80521 aagggcagag ccagtggccc tgcccttatc agttatcagg cctcaggttc ctgatccggt
    80581 accgggggta ccactacgcc ttcacgcggc gcttcaggat tcggcgctgg cagattcatc
    80641 aacttcgcca gaatgctcgc cagtttcagg cgcatttccg gacgacggac gatcatgtcg
    80701 atcgcgcctt tctcgatcag gaattcactg cgctggaatc taggcggcag tttttcgcga
    80761 acggtctgtt cgataacacg cggaccggca aagccgatta acgctttagg ttcagcgatg
    80821 ttgagatcgc ccagcatggc gaaacttgca gaaacaccgc ccatcgtcgg gtcggtcagc
    80881 acggagatgt acggcaagcc gcgctcctgc atttttgcca gtgccgcaga ggttttcgcc
    80941 atctgcatca gcgacatcag tgcttcctgc atacgtgcgc caccagaggc ggagaagcag
    81001 atcagcgggc agttatcttc ctgcgcctgc tcaacggcac gcacgaaacg tgcacccaca
    81061 acagacccca ttgaaccgcc cataaaggcg aactcgaatg ccgcagcgac aaccggcatt
    81121 ccatacagag tgcctttcat caccaccagc gcatcttttt cgccggtttc tttctgcgca
    81181 gatgccagac ggtctttata cttcttggag tcacgaaact tcagctcatc tttcggctca
    81241 agctcgctac ccagctccac aaggcttcct tcatctaaca ggctatgcag gcgattacgc
    81301 gctgtcatac gcatgtgatg gtcacacttc ggacagacct caagattacg ttccagctca
    81361 gcgcggtata aaacctgacc gcagctatca cacttagtcc acaccccttc aggaatgctc
    81421 gccttgcggg tgggagtaat gttgctttta attcgttcaa tccagctcat tagggacctt
    81481 tctgtctgaa cttggttcga tgccagtttt atctttgggg acgcataatg ccatttttgc
    81541 ccccaacaga ccatgaatgt tgcacattaa aacataacag cccgaaactt tggataaaaa
    81601 agtggtcgaa ccgcggagtt actttttatt ttgcggcgcg tgccgcagcg cgtttgtgac
    81661 ggattatttc gatgacgccc ggcaaaatag aaaccacaat aatcccgacg atcagcagct
    81721 taaggttatc ctgaaccatc gggattgtac cgaagaaata gcctgcgtag gtaaaaagca
    81781 gtacccacaa cagtgcgccg atcacgttat aagcagcgaa atgacggtac gacatgtggc
    81841 ccattcccgc aacaaacggg gcgaacgttc tgacgatcgg cacaaaacgg gcgagaataa
    81901 tcgttttgcc gccatgtttc tcataaaact gatgggtttt gtcgagataa ctgcgacgga
    81961 aaattttcga attgggatta ctgaataact tttcaccgaa caaccgcccg atagtgtaat
    82021 tgaccgcgtc acccacaatc gcggcgatca gcatcagcac taccatcata tggacgttga
    82081 gatcgttggt ttccagtgat gccaacgctc cggcaacaaa cagcaatgaa tcacccggta
    82141 aaaacggcat taccaccaga ccggtttcac agaacaaaat taagaacaaa atggcataaa
    82201 cccagacgcc gtactccgcg actagctctg ccagatgcac gtcaatgtgc aggataaaat
    82261 cgatgaggaa ataaatcagg tccatatttg cctatgcctt gtactcgtca tctttcaggc
    82321 tgtaactacg ttggctacag cctgaaatgt tccgagcatt attctcgtta gtccgccaga
    82381 aatagcgggc ccattggcgg ttttggaaga tcataccggt cagggtaatc caccgcgacc
    82441 agatacagcc cttccgcttt tgccgttgct gccgccagcg ttctgtcctt tgccgccagc
    82501 aactctgcta tccagctctc cggctggttg tgggcaccga cttccatcag gctgccgaca
    82561 atattcctga ccatatgatg tacaaaggca ttcgctttga tatcaaccac cacataagga
    82621 ccgtgacgcg tgacattaat atgcataacg ttgcgccacg gggttcggga ctggcactgc
    82681 accgcacgga acgaggtgaa atcattctcg cccagcaagc attgcgcagc ccgatgcatc
    82741 cgttcagcgt ccagcggttc gtaaaaatgg gttacccctt tactcagtac cgccgggcgc
    82801 agccgatgat tgtagatgat gtagcgataa cggcgagccg tggcgctaaa tcgggcatga
    82861 aaatcatcag gtacagcttt aacccaacgc acggcgatgt caccaggtaa attcgcattt
    82921 acgcccaagg tccacgccgc gtctttgcgc tgcgcggttg tttcgaaatg caccacttgc
    82981 ccggtaccgt gtacccctgc gtcagtacgc ccggcgcaga agacggtgat gggttcgttc
    83041 gccacctggg agagcgcctt ttccagcttc tcctgcacac tgcggacttc attctgccgt
    83101 tgccagccgt aatacttact gccgtcgtac tcaatgccca gcgcaatttt ataaactggc
    83161 agttgttgct ggtcggacat tagtacagat actcctgcac cagtttctcg gcgattttta
    83221 ctgccatcag cgcgccgcca aagcgaacgt tatcggccac cgaccagagc tggacttgct
    83281 ccggcatacc gtagtcatta cgcacgcagc caacagaaag atgcggcgta cccgaagcat
    83341 cccctacctg agtcgggaat tcgttctctt cagagagcac aatatcttcg ccctgagcaa
    83401 acgcatcacg cgcttcttct gccgccagcg gacgcagtgc ttcaaagttg accatctggg
    83461 catgaccgta gaataccggt gcctggacga cgcttgccga aatcatcagc ccttcgtcct
    83521 gcaggatttt gcgtacttcg tcaacgatac gacgttcttc acgcacgcta ccttcgctat
    83581 ccggcaacaa cggcagcata ttgaacgcca gttgacgacc gaagaaatct tcttcgtcaa
    83641 tcggaatgcc gttgagcaat ttcgcactct gccccgctaa cgcatcgacc gcttttttgc
    83701 cctgggcgga ggctgaaatc aggctggtaa cactgatacg cgacaaaccg ccctgatcga
    83761 ttaacggttt cagtgccgcc agcagctggc tggtcagact gtctggtacg gcgatgacat
    83821 tccggttgcg gtaatcggtc agtacaaacg ggtttacttc cggcaccacc agcggtacgt
    83881 cgggttcgag agcaaacagt ccgctgctgt cgatcaccag gcaacctgag ttggtcgctt
    83941 cttcaaccca ggcagcggta gcttctttgc ctgcgacaaa aaatgccagc tgcgcctgcg
    84001 tccagtcgaa ttcagcggca tcctgcacgg tgattgtttt accaccaaag cgcagttgtt
    84061 cgcctgcgct ttcgttacgt gccagtgcat aaatttcccc aaccgggaac tgacgttcag
    84121 ccagcgtttc aagcagggct tcgcccatag cgccagttgc gcccaggacg gcaatgttcc
    84181 agccttcaga catggtggtt tactccagaa atagcaaagc tccctgccaa tgttacagca
    84241 gagagcatga agaagagatt aacgtgccgg atgatgaacg gcgttaaaac ccagtttaca
    84301 cagcaatgat gccgcactgg cgtcatcaca aattacatac agagatgacc attcacggcg
    84361 ctcaagatag tttttgcgca gtttatcgaa ctcacccggt atcccggcga ctttacgcag
    84421 cggtgcgtca tcgcggcgca catcatacac caaatgcacc agccttttca gcgtcggttg
    84481 atcgagcgga ccatgcagcg taatgcggcc aaactcaggc gcgggcagca acgtatccag
    84541 cgcaacgtgt tgttcatgcc caataaactt gctataggct tcaaacactt gcgtcgtgcc
    84601 acgcgcttta ccctccaagg tatagcctgc gatatgcggc gtgccgatat ccacttttgt
    84661 cagcaattcc acattaagtt ccggctcgcc ttcccagaca tccagcacca cgcttaactt
    84721 ctggccttca ttcaggcagg tcagcagcgc ggtattatcg acgactgcgc cacggcaggc
    84781 gttaatcaga atcgctccgg gcttcaggct acggatcagt ttttcatccg ccagatgtag
    84841 cgttttgtac ggaccatctt taaagagtgg cgtatggaaa gtcagaatat ccgcgcgctg
    84901 gactaactca tccagcgagc ggaaatcacc ctcatcccca cggtcggcgc gaggcggatc
    84961 gcaaagtaag gttttaatcc ctaacgcttc cagtcgcgcc tgcaatcgac gtccaacgtt
    85021 accaacgccc acgatcccca ccgtacggtc gtgcagtgaa aatccatcgc gttcggcaag
    85081 catcagcagg gaggagaaaa catattccac caccgcaatc gcattacagc caggtgcagc
    85141 ggaaaaacca attcccgcct gcttcaacca tgcttcatcg acatggtcgg tccccgcagt
    85201 ggcagttcca acaaatttaa tgggttttcc tgccagcaaa gattcattca ctttcgtgac
    85261 cgaacgcacc atcagcgcat ccgcgtctgc cagttgagcg acggggattg gacgcccggg
    85321 aaccgcgatc acctcaccca aacggctaaa taagtcgcgg gcataaggca tattttcatc
    85381 aacaaggatt ttcacgtttg tgttacctgt atgagacgag agttaaccgg acaagtgtgc
    85441 cataatctcg cggccaggca tacttgcgaa gatttcaggt ataaggatac gtaatgatac
    85501 aacctatttc cggccctcct cttgggcagc caccaggtca gggagataac ctaccgtctg
    85561 gcgcgggcaa tcagccttta tccagtcagc aacgtacttc gctggaaagc ttaatgacga
    85621 aagtgacctc actgacgcaa caacaaagag cagaactgtg ggcgggtatc aggcacgata
    85681 ttggtctgtc gggagattca ccgctgcttt cgcgtcactt ccctgccgct gagcataatc
    85741 tggcgcaacg tctgctggcc gcgcaaaaaa gccattctgc ccgccagctt ttagcgcaat
    85801 taggggagta tttacgtctg gggaataatc gtcaggcggt cacggattat atccgtcata
    85861 actttggtca gacgccgctg aatcagctct caccggagca attaaaaacc attctcaccc
    85921 tgttgcagga agggaagatg gttattccgc aaccacagca gcgcgaggcg accgaccgtc
    85981 ctttattacc ggcggagcac aatgcgctaa aacagctggt gaccaaactt gcggcggcaa
    86041 cgggggaacc cagcaaacag atctggcaat cgatgctgga actttccggg gtgaaagatg
    86101 gcgagttaat tccagcgaaa ctgtttaacc atctggtgac ctggctacag gcgcgtcaga
    86161 cgctaagcca gcaaaatacg ccgacgctgg aatcactaca gatggcgcta aaacaacctt
    86221 tagatgccag tgaactggcg gcgttatcgg catatatcca gcaaaaatat ggcctttctg
    86281 cgcaatcatc gctttcttct gcccaggccg aggatattct taatcagctt tatcaacggc
    86341 gggttaaagg gattgatccg cgtgatatgc aaccgctgct taatcctttt ccaccgatga
    86401 tggacacgtt gcaaaatatg gcaacgcgtc ccgcgctgtg gatactgtta gtcgcgatta
    86461 tcctgatgct ggtctggctg gttcgttaac cccgacgaaa tgacagtatc gtgacaataa
    86521 tacccagcac cgcagagatc gccccggcaa gaaataccga agagtaacca aacgtggtcg
    86581 ccagcattcc cgccagcggc ccggagacgc cgagggcgat atcctgaaac gcggcgtaac
    86641 cgcccagtgc ggtgccgcga acttgtgagg ggacgcgttt aaccacctcc acgcccagcg
    86701 caggaaagat aagcgaacat ccggctccgg ttaacgccgc gcccgctaat gcgacccatg
    86761 cacctggggc ttgccagagc agcaacaagc ccaccgtttc tacaagcaga gagacaatcg
    86821 ccactttcac gccgccaaaa cggtccggca tccagccaaa catgacgcgc atcacgacaa
    86881 atgcgccgcc aaacgcggta agagtaaagc ccgccatcgc ccatcctttg ctggcaaagc
    86941 agagcgaaac gaaagtcccg ataaccgcaa aaccaacgcc ttgtagtgcc agccctaacc
    87001 ctggtttcca gataagcccg acaacgctcc acagcgatgg acgttctccc gccagggccg
    87061 gtactttgcg cactgtgccg ttacaggccc acgccagtaa gggtaatacc attgtggtga
    87121 tcgccagtgc ggcaaaaccg taatggctat gaatcaacag gccaagcggc gcaccaacag
    87181 cgagggcacc gtaaatcgcc attccgttcc atgacatcac tttgccagag tgttttggcc
    87241 ctacgatgcc taacccccag gtcagagcgc ctgtcagtaa ctggctttca ccaaacccaa
    87301 gaatcaaacg cccgatgacc aacagggcaa atttgaacgg tgcggagaca ggcaaaatcg
    87361 ccgccagcag caacgcgccg ccagccagac cacaagctaa catcccctga agcgccgaac
    87421 gttttgcgcc atattgatcg gccagtcgcc cggcgtaacc acgcgtcagc accgtagcca
    87481 gaaactgaat cccgacggca atgccgacca tggtattgcc atagcccaga tcatgatgaa
    87541 caaacagcgg gataaccggc aacggcaacc ctacggtcat gtaggtgaga aaaaccgcaa
    87601 aagcgatgcg gaagagcgaa aaactggcag aagatcgtgt ttcggtttgg cttacagcag
    87661 tcatgcatta ctccagaatg cagcgcaagg cgaggagtat ccccgtctca tctctctggt
    87721 ttcagggtta cggtgcgttg gcaggattta acgcgtacgt cttttcagaa ggaaatcgac
    87781 aaagcgggaa gtttgcctag aactggtggc gattgtcaat gatgtgaaaa agggaaccat
    87841 caggttccct tttgcgttag tgccggaggc cgcattggcg cgtaacgtcg gatgcgacgc
    87901 tggcgcgtct tatccgacct actccggcct actgcgaatt aatctttcag cttgcgcatt
    87961 accagcgtgg cgttggtgcc gccgaagccg aagctgttag acataacagt ggtcagttcg
    88021 cgatcggtcg tttcggtcac gatgttcaga cccgcagcct gctcgtccag ctcttcaatg
    88081 ttgatgctcg gggcgataaa gccgtgttcc agcatcagca gagagtagat agcttcctgc
    88141 acacccgcag cgcccagtga gtgaccggtc atggctttgg ttgcagaaat cgccgggctc
    88201 ttatcgccga acacttcacg gatagctgcc agctctttca cgtcgccaac cggagtcgaa
    88261 gtaccgtgag agttcaggta atcgattgga gtatcaacgc catgcatcgc catctgcata
    88321 caacgtactg cgccttcgcc agacggagca accatgtctg caccatcaga ggttgcgccg
    88381 tagccaacga tttcagcata gatgtaagca ccacgcgcca gcgcgtgttc cagctcttca
    88441 accactacca taccgccgcc gccagcgata acgaaaccgt cacggtgagc gtcgtaagta
    88501 cgggaggctt tttccggggt gtcgttgtat ttagtagaca gcgcacccat cgcgtcgaat
    88561 tcgcaggcca tttcccagca cagctcttcg ccgccgccag caaacacgat gtcctgtttg
    88621 cccagttgga tctgctctac tgcgttaccg atacagtgtg cggaagtcgc acacgcggag
    88681 ctgatggagt agttaacgcc atgaatttta aacggcgtgg cgagacaggc agaaacgccg
    88741 gatgccatcg ctttagtgac cacatacggg ccaaccgctt tcaggccgcg cgggccgcgc
    88801 attgcgtcag cgccgaacac ctggaaacgc ggggaaccgc cgccggaacc tgcaatcagg
    88861 ccaacgcgcg ggttattctg gtaagcttcc ggagagaggc ccgcatcagc gattgcctgc
    88921 tccatagaaa ggaatgcata aatggatgcg tcgctcataa agcgcacaac tttgcggtca
    88981 atgaggccag tggtatccag ttttatgttg ccccagacgt ggctacgcat gccggaatcc
    89041 ttcagctcct gagagaaagt gatccctgaa cgtccttcac gcagagatgc caggacttcc
    89101 tgctggttat taccgatgct ggaaacaatg cccaggccag taatcactgc acgtttcatt
    89161 caatacctct gtaagtcgca catagagtaa gtttcgaatg cacaatagcg tacacttgta
    89221 cgccgaacaa gtccgatcag ccatttaata gagaaatttg cgcagcctta cacacatcgc
    89281 taagatcgag ccaccgcctg taagacgagt aacttacgtg aaacactact ccatacaacc
    89341 tgccaacctc gaatttaatg ctgagggtac acctgtttcc cgagattttg acgatgtcta
    89401 tttttccaac gataacgggc tggaagagac gcgttatgtt tttctgggag gtaaccaatt
    89461 agaggtacgc tttcctgagc atccacatcc tctgtttgtg gtagcagaga gcggcttcgg
    89521 caccggatta aacttcctga cgctatggca ggcatttgat cagtttcgcg aagcgcatcc
    89581 gcaagcgcaa ttacaacgct tacatttcat tagttttgag aaatttcccc tcacccgtgc
    89641 ggatttagcc ttagcgcatc aacactggcc ggaactggct ccgtgggcag aacaacttca
    89701 ggcgcagtgg ccaatgccct tgcccggttg ccatcgttta ttgctcgatg aaggccgcgt
    89761 gacgctggat ttatggtttg gcgatattaa cgaactgacc agccaactgg acgattcgct
    89821 aaatcaaaaa gtagatgcct ggtttctgga cggctttgcg ccagctaaaa acccggatat
    89881 gtggacgcaa aatctgttta acgccatggc aaggttggcg cgtccaggcg gcacgctggc
    89941 gacatttacg tctgccggtt ttgtccgccg cggtttgcag gaagccggat tcacgatgca
    90001 aaaacgtaag ggctttgggc gcaaacggga aatgctttgt ggggtgatgg aacagacatt
    90061 accactcccc tgctccacgc cgtggtttaa tcgtacgggc agcagcaaac gggaagtagc
    90121 gattatcggc ggtggtattg ccagcgcgtt gttgtcgctg gcgttattac ggcgcggctg
    90181 gcaggtagcg ctttattgcg cggatgaagc ccccgcgctg ggtgcttccg gcaatcgcca
    90241 gggggcgctg tatccgttat taagcaaaca cgatgaggcg ctaaaccgct ttttctctaa
    90301 tgcgtttact tttgctcgtc ggttttacga ccaattaccc gttaaatttg atcatgactg
    90361 gtgcggcgtc acgcagttag gctgggatga gaaaagccag cataaaatcg cacagatgtt
    90421 gtcaatggat ttacccgcag aactggctgt agccgttgag gcaaatgcgg ttgaacaaat
    90481 tacgggcgtt gcgacaaatt gcagcggcat tacttatccg caaggtggtt ggctgtgccc
    90541 agcagaactg acccgtaatg tgctggaact ggcgcaacag cagggtttgc agatttatta
    90601 tcaatatcag ttacagaatt tatcccgtaa ggatgactgt tggttgttga attttgcagg
    90661 agatcagcaa gcaacacaca gcgtactggt actggcgaac gggcatcaaa tcagccgttt
    90721 cagccaaacg tcgactctcc cggtgtattc ggttgccggg caggtcagcc atattccgac
    90781 aacgccggaa ctggcagagc tgaagcaggt gctgtgctat gacggttatc tcacgccaca
    90841 aaatccggcg aatcaacatc attgtattgg tgccagttat catcgcggca gcgaagatac
    90901 ggcgtacagt gaggacgatc agcagcagaa tcgccagcgg ttgattgatt gtttcccgca
    90961 ggcacagtgg gcaaaagagg ttgatgtcag tgataaagag gcgcgctgcg gtgtgcgttg
    91021 tgccacccgc gatcatctgc caatggtagg caatgttccc gattatgagg caacactcgt
    91081 ggaatatgcg tcgttggcgg agcaaaaaga tgaggcggta agcgcgccgg tttttgacga
    91141 tctctttatg tttgcggctt taggttctcg cggtttgtgt tctgccccgc tgtgtgccga
    91201 gattctggcg gcgcagatga gcgacgaacc gattccgatg gatgccagta cgctggcggc
    91261 gttaaacccg aatcggttat gggtgcggaa attgttgaag ggtaaagcgg ttaaggcgcg
    91321 gtaatctgct ctcgcagtgt ttgccggatg cggcgtgaac gccttatcca acctacgtgt
    91381 gacgtgtagg cctgataaga cgcgataagc gtcgcatcag gcatggtgct ccagacgccg
    91441 caacttacag ctgtgacgcc tgctgaaaca ggttttccca catatcggtc accagcgcct
    91501 ggtcacgcgg cgacaattca ccggcaccaa tggctttttc cagactctgg ctaacggtcg
    91561 tatgcaccgc ctgagcggag tggtcatcac cactttccag ttctgcgatg gctaatgtca
    91621 ggtggccacg caaataccca ctggcaaaca actcatcatc actggcatgg tcaaccatgc
    91681 cgtcgattaa tgccagaatg cgtgattcaa actccgcgat catcttcttt cctcattgta
    91741 aaacgtggcg ctggcttcag ttgagcgctt ccggccacgg gaactgttcc gccgtaagtt
    91801 ccggcgtgtg ataataattt tgtagtgctt taatgaagcg tgccgggcgt tccggaatgc
    91861 catttgtcag ataatccatc acctgcgcat gtacccgccg ttgaaacacc acacgatccg
    91921 gttcgaaatc cccttccaga ttgtcgcaac tgacgttaaa cggatatcca gccgccacgc
    91981 agaataacca gtcgaacgcc tgcggcttca cttcaacatc ttcaaactgg ctttgcgtct
    92041 gggcatcgcg tccgtccggg caataccagt agccgaaatc aaccagctca cggcgcgctt
    92101 ttccggcaat acaccagtgc gaaatctcat gaattgcgct ggcataaaag ccatgagcaa
    92161 agacgattcg gttatacggt acttccgcat cagcaggaag atagatcggt tcgtcgtcgc
    92221 ctttaatcag acgggtatta aattcatcgg caaagcagct attaaaaatt tcaatcaact
    92281 gctcgtagtg gtgtgtactg ttcattagtt catccccaac cagtggagga tctcctgtcc
    92341 gtggctatcg taaagtaatt tggcactcat caccgccgag acgataacaa tcatcgggcg
    92401 gatcagcttt tgtcctttgc tcaacaccaa ccgtgaaccc atgcgcgcgc cgaggaactg
    92461 ccccaccagc atgacaaagc ccgtcgccca aatcactttg ccgccgagaa taaacagcag
    92521 cagaccgcca atgtttgacg ttgcgttgag taatttggcg tgagccgtgg ctttggcgag
    92581 gttgaatccg cacagcataa cgaaggccag cgcgtaaaac gatccggcag ccgggccaaa
    92641 gaatccgtca taaaaaccga cgcagccacc ggcaattaac gcaaagggta aaccgtacat
    92701 ccggcgctgg cgatcttctt cacccagctt tggcatcagc aagaaataga ggccgataca
    92761 aatcaccaga atgggcaaaa tctgccgtaa gacatcagcc tgaacgtact gtaccaacag
    92821 tgcgccgctc attgagccga caaaggtcat ggcaatattg agtttctgat cgcttaaact
    92881 aaccactttg cggcgaataa agtagatagt ggcggaaata gagccgccgc aggcttgcag
    92941 tttattggtt gccagcgcgt tagcgggaga cattcccgca gccatcaatg ccggaatggt
    93001 gagtaaccca ccgccaccgg caatcgagtc gataaatccc gccagcatgg cgacaaaaaa
    93061 gagaactccc agcaacagcg gagaaaccat aaacaggcta ttaaacgttt ccattagatc
    93121 acgtgctcat ccagtagcgc ctggcaggaa ggcggcaacg gaggcggtgt cttcttctca
    93181 ggctttgttg ttcccggttt tggaggttca aaccagcttt gcagttctgc cccgcaacca
    93241 tcgcctgatg gcggtaaagg ttgatcttca cactccagac tatcggcagg gcaacgtaat
    93301 cgtacatgca tatgcgcgcg atgctggaac cagggtcgca ctttgcgcaa ccagtcgcga
    93361 tcggtgcccg catcaaggca aagttgttgt ttaatcgccg gattaacaaa aatgcgcgtg
    93421 acgtctttgt cctgggcggc gagtttgatc aagctgaaaa tttctggctt ccacagcgtg
    93481 gagacaacgt gtttaccgtc gcgggaaact aagtccagtg cttgcgggcg caagagctgc
    93541 gcggaggtcc agcgagtttt cggcagttgc agaaagatat cgacatccag tccggtctgg
    93601 tggctggcat gaccgccgtt gaaacgccca ccagcgggca tccccatatc gccaatcagc
    93661 accgtaccca tgcccagatt gctcacctgg ctactcagac gctggataaa catcaccaga
    93721 tccgggtgac cgaaatagcg acgctgatcg gtacgcatga cctgataatg ttcggactgt
    93781 atcggcaacg tgtcagcgcc gacaatacag ccattagaaa aactgcctat cgattgtgcg
    93841 ctacccggca caggttgggt tattttttgc cacggcgtcg ctgccaggct agcgctactg
    93901 gcaagcagag ccagcagcgc aatcgcggtt ttgttcattt tttaccagcg tggaatatca
    93961 gtcttcacat cggcattttg cgcccgctgc cgtaacaggt gatccattaa aacgatcgcc
    94021 agcatcgctt cagcgatcgg cactgcacgg atcccgacac agggatcgtg acggcctttg
    94081 gtgatcatct caacttcttc gccaaagcgg ttaatagtgc gccccggcac ggtaatgctg
    94141 gaggtcggtt tcagcgccat atgagcaatg atttgctgcc cgctgctgat accgccgaaa
    94201 atgccgcccg catggttgct ctggaaaccc tctttggtga tttcgtcgcg gttctggctg
    94261 ccgcgcagcg ccactacgtc aaaaccgtcg ccaatttcca cgcctttcac cgcgttgatg
    94321 ctcatcagcg catgggcgat gtcggcatcc aggcggtcaa agaccggctc gccaagtccg
    94381 gcggggacac cactggcaac aacggtgact ttcgcgccga tggagtcgcc ctcttttttc
    94441 agcgcgcgca tcagttcatc taacgcgtcg attttgtcgg ggtccgggca gaagaacgga
    94501 ttttgttcaa ccagcgacca gtctttgatt tccagcggaa tgtcgcccat ctgggtcagg
    94561 cagccgcgga tttcaatacc aaatttctcg gcgagatatt ttttggcaat cgcccctgcc
    94621 gccacgcgca tggcggtttc gcgggcggaa gaacgtccac cgccgcgata atcgcgcaga
    94681 ccgtattttt gttcgtaggt gtaatcagca tggcctggac ggaagacgtc tttaattgcg
    94741 ctgtaatcct gagaacgctg gtcggtattt tcgatcaaca agccaatgct ggtgccagta
    94801 gtaacgcctt caaaaacacc ggagagtatt ttgacctgat ccggctcgcg gcgctgggtg
    94861 gtatagcgcg atgtcccagg gcgacgacga tcaaggtcat gttgcaggtc cgcttccgtc
    94921 agcggaatgc ctggcggaac accatcgacg atgcagccga gcgccagccc gtgcgattcg
    94981 ccgaaggtgg ttacgcgaaa gagttgtcca attgtgtttc cagccatcac ggctccgtta
    95041 ttgttgtgtt tgcgtgttta cttaatcttt ataaatcgcg aaatgttctc gtgcggcaag
    95101 aagctgctct ttagtgagca taaacacacc atcgccgccg ttatcaaact ccagccaggt
    95161 gaacggaaca tcgggatatt gttccataag atgtaccatg ctgttgccca cttcacaaat
    95221 caacacgcca tcatcagcaa ggtaatctgc cgcgttaccg agaatgcgac gcgtcagttt
    95281 caggccgtcg gtgccagatg ccaggcccag ttccggctcg tgacggtatt cgtttggcag
    95341 gtcggacata tcttccgcgt cgacatacgg cgggttagtg acaatcagat cgtattgcac
    95401 tttcggcaaa tcacggaaca gatcggaacg aatcggaatg acgttgtgga tcagaccgtg
    95461 ttcttcgatg ttctgttcgg caaccgccag cgcgtcggga gagatatcta ccgcgtcgac
    95521 ttccgcttcc gggaaggcat aagcacaggc aatggcgatg cagccgctac cggtacacat
    95581 atctaaaata tgctgcggtt gcttgctgat gagtccggca aatttattgt tgatcagttc
    95641 accaatcggc gagcgcggca ccagcacgcg ttcatcgacg taaaattcat gaccgcagaa
    95701 ccaggctttg ttggtcaggt aagccaccgg aatgcgctcg ttgacgcggc ggatcacgcg
    95761 ttcaacaata cggtgttttt cgctggaggt cagacgcgcg gtgcgcatat cttccgggat
    95821 atccagcggc aggtagagcg atggtaacac cagctgtacg gcttcatccc acgggttatc
    95881 ggtgccgtga ccgtaccaga tatttgccgc gctgaagcgg cttaccgacc agcgcaacat
    95941 gtcctgaatg gtttgcagct catttactgc ttcatcaacg aaaattttat ccacgtattc
    96001 ctccagggca tgatcgcaat aatttcggcg gctagtttgc catgaagatg acgataaatc
    96061 agcattcacg cgcggtgagt gaggaaaaat acgtttaaaa cgatcgattg tgctacgagt
    96121 cgggtaaact gtaggaaaat tagaaataga gacagataaa tgaaaaagaa aacaacactt
    96181 agcgaggagg accaggctct gtttcgccag ttgatggcgg ggactcgcaa gattaagcag
    96241 gacacgattg tccaccgacc gcagcgtaaa aaaatcagcg aagtgccggt gaaacgcttg
    96301 atccaggagc aggctgatgc cagccattat ttctccgatg agtttcagcc gttattaaat
    96361 accgaaggtc cggtgaaata tgttcgcccg gatgtcagcc attttgaggc gaagaaactg
    96421 cgccgcggcg attattcacc ggaattgttt ttggatttac acggtctgac gcagctgcag
    96481 gccaagcagg aactgggcgc gttgattgcc gcctgtcgcc gcgaacatgt gttttgtgcc
    96541 tgcgtgatgc atgggcacgg gaagcatatt ttgaagcaac aaacaccgct gtggctggca
    96601 caacatccgc atgtgatggc atttcaccag gcaccgaaag agtacggcgg tgatgcggct
    96661 ttgttggtgt tgattgaagt ggaagagtgg ttgccgccgg agttgccctg aatgctggca
    96721 gcaaatgccg gagcagcgta aacgccttat ccggcctaca aatatgtgat aattcaataa
    96781 attgcaattc acttcgtagg cctgataaga cgcatcagag caatactacg aacttcagtt
    96841 cattcaatac atactgaaaa cgacacgtcg aatcaatcgg ttgcccacta ctggtattca
    96901 cattcaccac aatatcgtgt agtactttat ccgttaccgg atgacgagtt ggcgtaaccg
    96961 ttaccgttga tttatcacaa ccgtaagagg taaaggttaa caattctgat accgcgcggc
    97021 agtatgctcc tctgccatca ttatcagaaa aattactgcc atcacagagt ctgccgcctt
    97081 ccatcaggta ggtcgaaagc gtggtggtca ctgttgaccc cgtggactga attcgtaacg
    97141 ccttattatg gctgacataa ttttcactcc aggagatatc gtatgaagcg accggtatcg
    97201 ttgggcatat tgtgttatgc actgtagggg ccgtatagct tccaaatccg ggtaaatctg
    97261 tataggaata aaaggatgcg atccaagggc aaccataatc atcagatgca acggtaattt
    97321 taaacgtcat atatgcacaa cgaaaaattt tcgtatctat gccagggcaa tttgagttat
    97381 taattgtatc aaagtcagtc agtaccaaag ttggagctga acccgaccca gaccaggaaa
    97441 taacccgcaa cgtatcgcct ttatacgcaa aagaatcata tttcccatat gcaacctcct
    97501 ttggcgttat caccgtaaca ttgaaaacac tgctcgctac agcaccggtt accttgtcca
    97561 tggcataata aactctcaca ctgtcaaaac tcgcactcat aagtttatct ccagcataac
    97621 tcttagatgc gccgacaaca ctcagaacga gcagcattag gctcgcgaaa attatggtcc
    97681 attttttcat ttcagaaata ctcaaaggtt gccgtcactg tggcactaaa gtctccggac
    97741 gttacatctc gcccgctctt tgcctgtaac taggtattaa aatttaggct gttatttccc
    97801 tgattcagcg ggagcgtgac gcctgttgta ttgttgattg gcaccgatgt gcctgccgca
    97861 gtttcaatac caatcccagc gccggatgct gatgacgagg agtcaaaggc cagaaagccg
    97921 ggtagagcac tatcttctgt tcctgtcaac gttactttga cattaaacaa tgtagaacta
    97981 tgacaatctt tcaattgaaa caccaatggc actcgctctg actgcccaag gttcattaaa
    98041 tcgctggcgg caatggcagg aaaatgtact tctgccaact ggctaccatt aatcactggc
    98101 gtacacgatt tactgattaa attgccggta aagtgcaaat tatctcctgc tattgtggtt
    98161 ccactgccca gcaacatcag gctactaata aaaaacaacg tacgcttttt cttcattggt
    98221 aatctacctg taacgtggcc caggcctcaa agtccgcctc tggcagaatg acaccactct
    98281 tttttactgg cacagccgtt aacaccggta aatcggtttc attcaccaca agtggcgtat
    98341 tgatcgtaaa actctgccct gcctgtttga gctgaatgcc taaaccagtg atattgctgc
    98401 ttactgcagc attatcaaaa tcgcttggtg tgccactcca gctcaaggtc atttccatta
    98461 atgggtcgcg aaccgccgta tcgcaggtaa tggaatacgg cacggtttgc tcataattgc
    98521 tgccattaat gtcatcaata agcacatcct gaaaagttat ttcaatggtt tgagcgttgc
    98581 taatcgtgca gttaggcggc tgcactaaat aaccgtgaaa agagacgttt gagagtgcca
    98641 tcgagcaaaa gccccacagc aaaataagcg agatccgttt cattggtaat ccaccacaat
    98701 tgtggcactg gcgttaaatt cagctgccgc taacgttgta ccactctgtt tcaccggcac
    98761 agcttccaga acgggaactc ccgaggaaaa attaaatggc aaccatgagc cagaggttaa
    98821 gtcgagtatt gtgtgatcac tacttttctg aacccgaata ccaaacccag gaatacccgt
    98881 gctgataact gtttcaccgt taatcacgac cgttgtcgcc tgcatctgta gacgcaggtc
    98941 atccattgcc agattgttac aaacaagcga gtagtcaatg ggacgtttat aatcaaccct
    99001 attgatctta ttaatgaata cattaccaaa ctcaactgcc gcaccattta ccgtacaagg
    99061 aggaggggca tcgacaagca cccgtaaatt catacttact gacttactcg ctgcaactgc
    99121 aaagccgcta ccaacacaac atatcagtaa cccagttacc gcccagacag aacaagatcc
    99181 tttcatactg gctctcccgg cctcagcctt tgcggctctg ctcttcatcg acctagcaac
    99241 tgttaccgtt gcaagtgaaa aagagcggca tccgagcgcc atagtcgttg acataggtca
    99301 gtaccggcac gctacccatc ttcacattca gcggtgcaga ggttttcggc gacatcacta
    99361 ttggcgagaa accggctgcc ggattgccat ttttctgtgt gctggcatta ctgaaaatga
    99421 cgtaatacga cgtcgggtta ttgacggtaa attcctgacc gttgcgggtt aaagtaacct
    99481 tatattgata ggggtttttc atgctgacgt tctccagcgc cttcggtcgc cagaacaact
    99541 taatacgcgt ttgtagtgct atttgcagcg tgttggcttt gtttgatttt ggcgggatct
    99601 cacgcacgtt gaagtagaac aggctttcac gatcggcagg caatttgttg atatccggca
    99661 ttccctgcac ttttacctgc ccgttcatca ttgaatcgat gcgttgcacc ggtggcagaa
    99721 cagttaacgg tgaactgatc ttgttaccct tttcatcttc gatccagctt tgggccagat
    99781 aaggcagttt cggatcgtta ttgcgcaacg tcacgctgat tgatttgtcg ctttcattaa
    99841 atatcaaacg ggtacgatct ggcgttacag aagcctgggc agacaaactg gcagcgctaa
    99901 gcagtaacgc tagcgtcgta gcacccaatt tggcagagca aagtaaatca gacatggatc
    99961 attccttaat cacgttattg gttagctgaa acagaaacag ggggattggt gggtgtcacc
   100021 tgttgcgtct gctcctgaat gacaggcttt atgtcatcag gaacaaccgg cgcgacgttg
   100081 cctttatgct ggcatggcag taacaagccg ttaaacagat cggcaggcag cgggtccggc
   100141 aggttgatat cgcaatgcgc aacaccactc cagaacacac tcatgtgttc gccaggtttc
   100201 accccagcca gataaacatt gccgtcatca tcgacaaggc ccactgtctg ctcgttatca
   100261 ttttttactt ctgcgccaaa cggaggatgg ctgccgtctt gtaaacgcag caccgccatc
   100321 gctttttgac cactgatgac ggcaaatttg cggtagccaa tcgccccttc agttagcgtg
   100381 gcttgtacca ccgactgggt tgcttcggcg ttttccggca gtttgttgag gtcgatatac
   100441 gcctgattgc ggtaatagtt attgacgtca gaaacgacgg ctttaccaaa catattggta
   100501 taaacagcag caccgttacc ttctaccgga acatcggcaa cgccatcggc atcaatcaac
   100561 aagcgtgtcc cgcccatatt ctgggtacgg tgaagtgcgc caccgtgggc agtaaacgtt
   100621 gcgccgccct gtaacgacaa gcccgcagag gtgtattgcc cttcatggta gttcgcactg
   100681 aggtcaacct gcgccagcga accatcatgg ctgtaatagc cgtcaacgct ggtgtgtttg
   100741 tcactggtgc cgacgttcaa ctgatagtga gtcgcgtcat cgacacggct gaaataaccg
   100801 acctgactgc tgtccgtccc actgccatag taccgttata gctaacggta ctgttgtcgc
   100861 cccacggcat actgagcgaa atgtacatgc ctttgtcggc ctggttgtca tactcgtaac
   100921 ggtagccagt catcgagatg ctgacattac gaatactgcc catattgaaa tagtgcgaga
   100981 gcatgatgtt gtagtttgtc tgctcctcgc gatcccagta ggtatggcgg gtatagttga
   101041 gataaaccga aacacccgca tcgcggaagt tctggttata agtggcggtg tacatctctt
   101101 tgtcgttgcc cgtgcgtacc attccgctgt cactggcatc cagatactcg ctcatggtca
   101161 taaagttctc ttccgagaag cgatatccag caaaagtaac gcggctgttg agctggtcaa
   101221 agtctttgga ataactcaca cggaaggagt taccgtccag cgaacctttg ccataagcgg
   101281 tgtctttatc cagtttggtg tgcgagtgag taacatcaaa cgcgaccgcg ccgaatgtag
   101341 acaaatcgcg accgacacca agcgccgcag actgatagtt ttcatctccc agtgcgccgc
   101401 catataacga ccagccgtta gcgatccccc aggaagcttc agcaccagaa aaaaattcac
   101461 cctcgacatg gtgtccccac tcttgcggac ggcccatcat gatcttatag cgaacctgac
   101521 ctggacgagt gaggtatggc atcgaggcgg tgctgatgtc atattcctgc acctggccgt
   101581 tctgttcttc aatgcgaata tgcaacgtac cggagacaga atcaccaaga tcctgaatac
   101641 gaaacggtcc agccggaacc tgcgtttcgt aaatcacacg ccccatctgg ctgacggtca
   101701 cttttgctgt ggtgtgtgcc acgccggaaa tgtctggcgc gtagccgcgc agattgggag
   101761 gcaacatttg atcgtcagta ctgacactgc caccaacata gttaaaacca tcaaaaatat
   101821 cggatctgag gtaatcctcg cccagcgcca gtttggcttt taatgatggt aacgcccgcc
   101881 tggcatagta gcgactccac tcccattttt tttgagtttc atcgccgctg aattcatcgt
   101941 catcattact gcgagtatgt tgatagttag tctgccagtc agcacgcata cgccacggcc
   102001 ctaggttaac cccaaccgtc ccgttgccgc tgatctcgtt actatcatca ccgccatttt
   102061 cttcgtgccg ggtttgtgcg ttgatgctgt agtccgcgac gatcccggag atgccgtcat
   102121 cccaacgtga aggcggatcc cagtcgggat aagtatattc gaggtaagcc tgtggcagtg
   102181 aaatgactaa tgcggactgg cttaaatcag ccttaatttc catgccttcc agttgaccgg
   102241 atttcaggca tttagcatcg tggctccatt gcagattttt cgccacgtct tcttttaaac
   102301 caaactgcgc taccagttcc ggtgtcagac aagcatagct tttgctcgcg tcatcttcac
   102361 cagcatacca gtaaatatcg tactcttccg ccaatggctg tttatttagt tgaacctgta
   102421 aattgtattt tccgggctca acatatccct ggctggaaaa acgcttcaga tcaatttttg
   102481 tgtcgccttt taattccaga aaacgggaat caaactgaat gtcgtcttca gcccagacac
   102541 tgctataact ccctgacatt gccagcgcaa tgcaccacgg aagaatccgt aatcgaaaaa
   102601 gagaatggtc aggcatacac gtttctatcc gtttttattc ttgttcggtc aaaatccatt
   102661 taaatccccc atagcagtgt cgctatgggg gtatgcctta caggtaagta atctggaagg
   102721 tggtatttgc ttcaaaatta cccagatctg gcgcatcagc tgcgccgacc agccaggctt
   102781 taaagttcag tgtctgcttc gctttgcctt tgttggtaga agtgtctttc actattttct
   102841 ggtcaatacc catgccgctt ttgtaagagg tacccagaga gtcaccgatc gccaggctaa
   102901 cattgttaaa tgccgcaccg gtatcggtat tgaaaatggt gtaataattg ccgctatttg
   102961 cagaagaaac ggtaccagta aaggtagtgg tcattgtttc ctgagtatca aatacgcaat
   103021 cttgcagacg aatctggaaa cttttcggcg tggtggtgcc gttatttttc agtttttcgg
   103081 caccgatatc acccatatca acttccagtt tgtgatcgtc cggaacgata gaacacactg
   103141 agtcttcaat ggtgccgtaa aaacgtgcag taccgttgtt acccgcagcg attgttgccg
   103201 tagaggtgaa cacgcccatc accattgccg cagcaatagc tgttttaaca aacttactca
   103261 ttatttgtcc ttaaactata aataattaaa aatataaaca ttgcaataca ttgattcagt
   103321 caatagccaa tgtttatacc cagccattga atacggcatg cgacccaaaa agaacaggtc
   103381 gtagaaaatt gtattcattg gtgtggggcc gcatagtgat gtttccacac ccctgatcca
   103441 atttaatttt actaaagagt aaatgtagat ttaattaata tattgacaga agaaaggtta
   103501 tcttaaggac atccttaaaa aatacggaat aagtatttag aaaaaataga aagtatatca
   103561 ataatgaata tcaacagctt aaaaaacaaa caatgaacat ataatgcgat tggcatttat
   103621 ccacttttga caatataata tataaacact atagccagaa tttaatcaag tttcaattac
   103681 atcctgcctg attattcaac tctattttct ctattatctg tattaataaa tgacactgag
   103741 tgtcaaacag acattattca tttcaacatg taactttggg caaaatcaag atacgcgcag
   103801 aagaataatt tccgaatgaa tgtagaaagt gttttattta tagataagga gagtcttcga
   103861 tagtagtgag gttgacagat atccgctgcc taatgagcaa ttatgcccaa tgaatctacc
   103921 tcattgggca taatttggaa cacatcagat agcttttgcc atcttcagat tacacgggct
   103981 catctgccag ttgaatgtac cgtttccgct ttcatcaagc gttacgctgg caatggccga
   104041 ggtggtaaac ataggcggcg tttcgcccgg acataactcg gcaaccagat acccgactaa
   104101 cggtaagtga gagatcacca gtaccgaagc aacaccttca ttggtcaacg cctgcaaata
   104161 ggcactgacc agaccaacat cgccgcaggg cgttaactcc ggcagaactt ctgcactgga
   104221 aggcaggttc agacaatccc ctacttcttc cagtgtttgc tcggctcgca ggaacgggct
   104281 caccagaacg cgttcgattt ccactttttg acctttcagc cagttcgcca tcaggcgaga
   104341 ttcgtcacaa ccattagtgg tcagaggacg aaccgaatca ctggcggcat cgagggctgc
   104401 gtcgccgtga cgcatgataa aaacttgcat attgcaccgc ttttgttaac cagattcgcc
   104461 cgttttcttt catctgaaac cagagaaaga aaacggtggc cggcattgtg ccttatccat
   104521 tcaccgaatg aaacgctgtt ttttacctca atggcgtaag tatagtcaat atgcgtttac
   104581 attttgacca acactccgcc attcagcgcg gattcatata gctttgacct tcttattgca
   104641 ggtcagttgc agttgttttc caaaaacttt ccccacgcgc gcccatctcg accaaacgct
   104701 cgcaaggggt aaaacgggaa ccatactgcg tggccagtcg ttgcattatt gcaaccactt
   104761 cgcccgcgcc gagagaatcg atatagcgga acggtccacc gagaaatggc ggaaaaccaa
   104821 tgccaaatac cgcaccaata tccccgtcac gcacgctacg gataacctgc tcatcaacac
   104881 aacgtactgc ttcattcagc atcaacatca cacaccgttc agcaacctgc ggtgcggaga
   104941 gtcgcccctg cccttgtgcg ccaatgagcg ggtaaatggc aggatcgacc tgttttttgc
   105001 ttttacgccc tttctgacca taaagataga aaccccgacc attttttctg cctttgcgat
   105061 cgtcgttcaa aattgaagaa acaacatttg caggcgcgct aaaacgttct ccataagcgg
   105121 cttccagtac aggaataatt ttagtcccgg tgtcgattcc tacctcatcc aaaagttgga
   105181 ttgggcctac cggaaaacca aatttcacta gcgcggcatc aatgtgctct acccgttcac
   105241 cttcggtcag catgcggata gcttcattaa tgtaaggcgc taagatgcga ttgacgtaaa
   105301 acccggcttt gtcacgcacg acaatgggcg ttttgccctg ttttttcgcc agttttaccg
   105361 tagtggcgat ggtttgcgcc gatgtacccg catgaggaat aatctctacc agcggcattt
   105421 tttccaccgg actgaagaaa tgcaggccaa taacttgctc aggtcgcgcg gcgtgagcgg
   105481 cgatatcacc aatcggtaaa gatgacgtgt tcgaagcaaa aatggtatga gcggcgcaat
   105541 tttgctccac ttccgccacc atctgttgtt ttaattcgag attttcaaac accgcttcaa
   105601 taatcagatc gcgatgggca aagccgcgat agtccgtcgt tccggagatt aatgccagct
   105661 gtttgtcacg ttcgctggct ttgagatgac gacggcgaac tttgccctcc agctgatccc
   105721 aactgtactt cagcgcatga tttatgccct gcgggttgat atctttaatt ctgaccggaa
   105781 gccccgcttt acaagcagtg acataagcaa taccgccgcc catcaagcca ccacctaaaa
   105841 ttcccacgct gtttaatggc gcaggcggtg catcactacc gggatctttc ttcacgtccg
   105901 tactggcaaa aaagatacta cgcaaagctt gcgattgcgg cgtcatcgcc agttcgccaa
   105961 aggcgcgagc ttcagcgtca tagccgctgc tggtgccctg cgctaatccc gtttcaacaa
   106021 cctccaggat gcgttctgtc gccggataat tgccttgagt tttgtgttct gttttcttgc
   106081 cgaccatttt gaacagcagc gcacgaccta acggccccgc cagaatacgc tcgcgtacag
   106141 gtagagggcg ggaagatggg cgatccttct tcgccagctc aacagcggct tccagcaaaa
   106201 tggagtgcgg aacaacgtca tccaccagcc ccagctttaa tgcctgtttc gcccgaagtt
   106261 gttttccggt gagaatcatc tctaatgctg tactgacgcc tatcagacgc ggtaaacgct
   106321 gggtgccgcc tgaaccgggt aacaatccaa gttgtacttc aggcaaaccg agcaccgttt
   106381 tgggatcgtc agtacaaacg cgaccgtggc acgccagcgc cagctccagc ccaccgccca
   106441 ggcaagcgcc atgaatagcc gcgataaccg gaatgggcaa agcatgaatc tccgccatca
   106501 actgttggcc ctgccgcgcc agtgcttccg cttcttgcgc cgttttgcag ttgccaatca
   106561 tgttgatgtc tgctccggcg ataaaattgt ccggtttcgc ggagataaac accacgccgc
   106621 gcaactcttt gttttcacgg agttgcttaa taatggcgcg cacctgcgag gcaaattccg
   106681 ccttcagggt attcattttc tcatccggta cgtcgatggt gatgatagca atgttgtcca
   106741 gacgaacatt aagagtaaac gctgatgcca tttccattat tccgcctcca gaaccattgc
   106801 cgcgccaagc ccaccggcag cacaggcggt aactaaacca aatccaccgc cgcgacggcg
   106861 aagttcatgc aatgtctggg taatcatccg cgcgccggtc gccgcgaagg gatgtccgta
   106921 agcaatcgaa ccgccaagca cgttaaattt gctatcgtcc acttcgccag tggcatgtgc
   106981 acgccccagt acttcacgag caaaacgttc actacccagc aactgaatat tcgccagtgt
   107041 ctgagctgca aaggcttcgt gcatatcgat caatgtcagt tcgctcatcg tcaaaccggc
   107101 acgttccagc gccagcggtg ttgaccaggc tggaccgagc aacatgtcct gccagacatc
   107161 aatcgcagta aatgcgtagc tgcgcagata ccccagcggc accagcccta attctttcgc
   107221 ccgggattca gtcatcagga tcaccgctgc cgcgccatcg gtcagcggcg tactgtttgc
   107281 cgccgttacc gttccgtgtt tgcgatcaaa cgccgggcgc agctttgcgt aatcggcaag
   107341 cgaggaatta ccgcgaatat tgttgtcttc gacaagcggt tgtttataag gagggataaa
   107401 ggcagtcatc acctcttctt tgagttttcc gtctgaccat gcctgagcgg cacgctgatg
   107461 cgaacggtgc gctaatgcat cttgctgttc tcgggtgatg ccgtaggttt tcgccatttg
   107521 ttcggcggtg tcgcccatcc gcaagccagt agaatattct gctaccgcag gcggtacggg
   107581 cattaagtcg cgcaaacgca ggcgagagaa gagtttcagg cgctggctca tggttcgagc
   107641 tttgttgaca tcaaccagca cgtgcgccag ttttttactg acgccaattg gcaatacgga
   107701 agaggaatct gccccaccgg caatccccgc tcggatagtt cccgccatca ggctttctgc
   107761 gacgtttgca accgcctgga aactggtagc gcaagcgcgg ctgacgctgt aagcatcggt
   107821 atgcacattc attccggtac cgagaacaat ttcacgcgca atgttggggg cttcaggcat
   107881 ttgtacgacc tgaccaaaga ccagctgttc aatcacttca gcggggatct cgctgcgtgc
   107941 cagcagttcg cctaccacca tcttccctaa atcaaccgcg ggaatgccat gaaaagccgt
   108001 cgcctggcgg gcaaaaggcg tacgtaaacc gctaacaatg gcgatacgat cgccctggcg
   108061 ggtaaccagt ggtaaaacct gacccataac actcccctgt aaaaaaataa ataaagtggt
   108121 ctgacctgat catagtatta accatttttt tacatttagc caagcggaga aaaaggaaag
   108181 tgggagctat gacacagtga agacagaaga aagaaaagaa aacgcccctg ccagcaactg
   108241 acaggggcgt acgtacccga gaggaattaa cgcagaccca gctggaagat cagcatttca
   108301 gcttcgcagg caaaagtgaa atcgatatcc aggcgcacac cgtcagactc ttcagtgaaa
   108361 gtcggggaga ttttgcacgg ttcggattcc acgctacggg atttttcagt cagcgccgcc
   108421 agcgtttgtt ctgcttctgc gcggtttgca aacacgcggc tgtaagacgc ggtgcagtcg
   108481 gagttgtcca taatggtgcc aacatccata cagcagcaaa ccggggtttc atcagcacta
   108541 catttactca tcgttgattt cctctgtatg tgcacccaag gtgccagata aacgttgtgg
   108601 atattttacg cttccggaaa gtgctgctcc agttgttaat tctgcaaaat cggataagtg
   108661 accgaaatca cacttaaaaa tgatctaaaa caaaattcac ccgaatccgc gagtgcgcaa
   108721 cctccaaatt ttgccagctg gatcgcgttt cttagatcat atttgaaaaa agatggaaac
   108781 atacttgcaa cattctagct ggtccgacct atactctcgc cactggtctg atttctaaga
   108841 tgtacctcag accctacact tcgcgctcct gttacagcac gtaacatagt ttgtataaaa
   108901 ataaatcatt gaggttatgg tcatgagcca gaaaaccctg tttacaaagt ctgctctcgc
   108961 agtcgcagtg gcacttatct ccacccaggc ctggtcggca ggctttcagt taaacgaatt
   109021 ttcttcctct ggcctgggcc gggcttattc aggggaaggc gcaattgccg atgatgcagg
   109081 taacgttagc cgtaaccccg cattgattac catgtttgac cgcccgacat tttctgcggg
   109141 tgcggtttat attgacccgg atgtgaatat cagcggaacg tctccgtctg gtcgtagcct
   109201 gcaagccgat aacatcgcgc ctacggcatg ggttccgaac atgcactttg ttgcaccgat
   109261 taacgaccaa tttggttggg gcgcttctat tacctctaac tatggtctgg caacagagtt
   109321 taatgatact tatgcaggcg gctctgtcgg gggtacaact gaccttgaaa ccatgaacct
   109381 gaacttaagc ggtgcgtatc gcttaaataa tgcatggagc tttggtcttg gtttcaacgc
   109441 catctacgct cgcgcgaaaa ttgaacgttt cgcaggcgat ctggggcagt tggttgctgg
   109501 tcagattatg caatctcctg ccgggcaaac tcctcaaggg caagcattgg cagctaccgc
   109561 caacggtatc gacagtaata ccaaaatcgc tcatctgaac ggtaaccagt ggggctttgg
   109621 ctggaacgcc ggaatcctgt atgaactgga taaaaataac cgctatgcac tgacctaccg
   109681 ttctgaagtg aaaattgact tcaaaggtaa ctacagcagc gatcttaatc gtgcgtttaa
   109741 taactacggt ttgccaattc ctaccgcgac aggtggcgca acgcaatcgg gttatctgac
   109801 gctgaacctg cctgaaatgt gggaagtgtc aggttataac cgtgttgatc cacagtgggc
   109861 gattcactat agcctggctt acaccagctg gagtcagttc cagcagctga aagcgacctc
   109921 aaccagtggc gacacgctgt tccagaaaca tgaaggcttt aaagatgctt accgcatcgc
   109981 gttgggtacc acttattact acgatgataa ctggactttc cgtaccggta tcgcctttga
   110041 tgacagccca gttccggctc agaatcgttc tatctccatt ccggaccagg accgtttctg
   110101 gctgagtgca ggtacgactt acgcatttaa taaagatgct tcagtcgacg ttggtgtttc
   110161 ttatatgcac ggtcagagcg tgaaaattaa cgaaggccca taccagttcg agtctgaagg
   110221 taaagcctgg ctgttcggta ctaactttaa ctacgcgttc tgataacgcg ttcgcctgga
   110281 taaagtcacc tgctatgcag gtgactttaa ctcccccact tcaccgaaag tagtttcctc
   110341 attgcttgcc tcctgagttt tgcaaacagc ctgatggcag cttgcccttt caaatcagta
   110401 agtggtatcc acagacaaac ctgaagggaa atgcatattt tcaagcgttc tgctcgtcct
   110461 tctcaaagag tttacttttc tgcatttcca ggatactcaa cccccctggc tattgtgcgc
   110521 ttatacactc aaattaaaga taggttctaa ataaatgagc gttttttgat agtctatttc
   110581 attaggtaat atatatttgt aacaaatcaa tcaacataga ataaaatcat gctaccatct
   110641 atttcaatca acaataccag cgcagcttac ccagaatcca tcaatgaaaa taacaatgat
   110701 gaaattaatg gattagtaca agagttcaaa aaccttttta atggtaagga aggaataagc
   110761 acctgtatta aacatctact tgagcttata aaaaacgcca tacgagtaaa cgacgatcct
   110821 tatagattta atattaataa ttcctcagtt acttatattg atattggctc caatgataca
   110881 gaccatatta ctattggtat cgacaaccaa gaaccaatag aattacctgc gaactataaa
   110941 gacaaagaac tcgtccgtac tatcattaat gacaacatag ttgagaagac tcatgatatc
   111001 aataacaagg aaatgatctt cagcgcatta aaagaaatat atgatggaga tcctggtttt
   111061 atcttcgata agatatcaca caaactcaga catacggtaa cggaatttga tgagagcggg
   111121 aaaagcgaac caacggactt atttacctgg tacggtaaag ataaaaaagg cgactctctc
   111181 gctattgtaa ttaaaaataa aaacggaaat gattacttat ctctcggtta ctacgatcag
   111241 gacgactacc acattcaaag aggaattcgt attaatggtg atagtctcac ccaatattgt
   111301 agtgaaaacg ccaggagtgc ttcagcgtgg tttgaaagca gtaaagctat catggcagaa
   111361 tcatttgcaa ctggttccga tcatcaggtt gtaaacgagc tcaacgggga aagactgaga
   111421 gaacctaacg aagtttttaa acgtttaggt cgagcaataa gatataattt tcaagtggac
   111481 gatgcaaaat ttagacgcga taatgtaaaa gaaataattt ctactttatt cgctaacaaa
   111541 gttgacgttg accatcctga aaataaatac aaagacttta aggatctcga agataaagtt
   111601 gaaaaaagac ttcaaaattg ccaggcgaaa tatcaaaatg aaattaatca actatctgca
   111661 ctaggtgtta attttgatga tatttaaaat tatttttcca gttttttgat ttttcaacgc
   111721 ctgaatatcg gaaatagtca gtgacaaa
//
LOCUS       DADMGC010000002        97951 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.002, whole genome shotgun sequence.
ACCESSION   DADMGC010000002 DADMGC010000000
VERSION     DADMGC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 97951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 97951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.002"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..1109
                     /gene="pdeF"
                     /locus_tag="JNZ73_000107"
     CDS             <1..1109
                     /gene="pdeF"
                     /locus_tag="JNZ73_000107"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY8730532.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
     gene            complement(1148..2689)
                     /gene="ppx"
                     /locus_tag="JNZ73_000108"
     CDS             complement(1148..2689)
                     /gene="ppx"
                     /locus_tag="JNZ73_000108"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HAY8730533.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLLRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPSTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(2694..4760)
                     /gene="ppk1"
                     /locus_tag="JNZ73_000109"
     CDS             complement(2694..4760)
                     /gene="ppk1"
                     /locus_tag="JNZ73_000109"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HAY8730534.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFGHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(4932..5570)
                     /gene="purN"
                     /locus_tag="JNZ73_000110"
     CDS             complement(4932..5570)
                     /gene="purN"
                     /locus_tag="JNZ73_000110"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAY8730535.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQSYAADE"
     gene            complement(5570..6607)
                     /gene="purM"
                     /locus_tag="JNZ73_000111"
     CDS             complement(5570..6607)
                     /gene="purM"
                     /locus_tag="JNZ73_000111"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAY8730536.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            6932..7558
                     /gene="upp"
                     /locus_tag="JNZ73_000112"
     CDS             6932..7558
                     /gene="upp"
                     /locus_tag="JNZ73_000112"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAY8730537.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            7644..8933
                     /gene="uraA"
                     /locus_tag="JNZ73_000113"
     CDS             7644..8933
                     /gene="uraA"
                     /locus_tag="JNZ73_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAY8730538.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            8983..9729
                     /gene="hda"
                     /locus_tag="JNZ73_000114"
     CDS             8983..9729
                     /gene="hda"
                     /locus_tag="JNZ73_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HAY8730539.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(9862..10221)
                     /gene="arsC"
                     /locus_tag="JNZ73_000115"
     CDS             complement(9862..10221)
                     /gene="arsC"
                     /locus_tag="JNZ73_000115"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HAY8730540.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(10242..11705)
                     /gene="bepA"
                     /locus_tag="JNZ73_000116"
     CDS             complement(10242..11705)
                     /gene="bepA"
                     /locus_tag="JNZ73_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HAY8730541.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSNLLDEWAKGNVLQQRAAQYGRALQAMKANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLEQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            11918..12979
                     /locus_tag="JNZ73_000117"
     CDS             11918..12979
                     /locus_tag="JNZ73_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY8730542.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(13017..13865)
                     /locus_tag="JNZ73_000118"
                     /pseudo
     CDS             complement(13017..13865)
                     /locus_tag="JNZ73_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(13887..15899)
                     /locus_tag="JNZ73_000119"
                     /pseudo
     CDS             complement(13887..15899)
                     /locus_tag="JNZ73_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
     gene            complement(15929..16342)
                     /gene="hyfJ"
                     /locus_tag="JNZ73_000120"
     CDS             complement(15929..16342)
                     /gene="hyfJ"
                     /locus_tag="JNZ73_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HAY8730543.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            complement(16335..17093)
                     /gene="hyfI"
                     /locus_tag="JNZ73_000121"
     CDS             complement(16335..17093)
                     /gene="hyfI"
                     /locus_tag="JNZ73_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HAY8730544.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(17090..17635)
                     /gene="hyfH"
                     /locus_tag="JNZ73_000122"
     CDS             complement(17090..17635)
                     /gene="hyfH"
                     /locus_tag="JNZ73_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HAY8730545.1"
                     /translation="MLKLLKTIMRAGTPTVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(17645..19359)
                     /gene="hyfG"
                     /locus_tag="JNZ73_000123"
                     /pseudo
     CDS             complement(17645..19359)
                     /gene="hyfG"
                     /locus_tag="JNZ73_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            complement(19349..20929)
                     /gene="hyfF"
                     /locus_tag="JNZ73_000124"
     CDS             complement(19349..20929)
                     /gene="hyfF"
                     /locus_tag="JNZ73_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HAY8730546.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFFFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(20934..21584)
                     /gene="hyfE"
                     /locus_tag="JNZ73_000125"
     CDS             complement(20934..21584)
                     /gene="hyfE"
                     /locus_tag="JNZ73_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HAY8730547.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(21596..23035)
                     /locus_tag="JNZ73_000126"
     CDS             complement(21596..23035)
                     /locus_tag="JNZ73_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
                     /protein_id="HAY8730548.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISDYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(23063..24004)
                     /gene="hyfC"
                     /locus_tag="JNZ73_000127"
     CDS             complement(23063..24004)
                     /gene="hyfC"
                     /locus_tag="JNZ73_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HAY8730549.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGQGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGLASALLRLHGSSG"
     gene            complement(24015..26032)
                     /gene="hyfB"
                     /locus_tag="JNZ73_000128"
                     /pseudo
     CDS             complement(24015..26032)
                     /gene="hyfB"
                     /locus_tag="JNZ73_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(26032..26649)
                     /gene="hyfA"
                     /locus_tag="JNZ73_000129"
     CDS             complement(26032..26649)
                     /gene="hyfA"
                     /locus_tag="JNZ73_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HAY8730550.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(26902..27372)
                     /gene="bcp"
                     /locus_tag="JNZ73_000130"
     CDS             complement(26902..27372)
                     /gene="bcp"
                     /locus_tag="JNZ73_000130"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAY8730551.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(27372..27944)
                     /locus_tag="JNZ73_000131"
     CDS             complement(27372..27944)
                     /locus_tag="JNZ73_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HAY8730552.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            28090..28968
                     /gene="dapA"
                     /locus_tag="JNZ73_000132"
     CDS             28090..28968
                     /gene="dapA"
                     /locus_tag="JNZ73_000132"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAY8730553.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            28985..30019
                     /gene="bamC"
                     /locus_tag="JNZ73_000133"
     CDS             28985..30019
                     /gene="bamC"
                     /locus_tag="JNZ73_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HAY8730554.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPASLEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            30232..30945
                     /gene="purC"
                     /locus_tag="JNZ73_000134"
     CDS             30232..30945
                     /gene="purC"
                     /locus_tag="JNZ73_000134"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAY8730555.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            31113..31976
                     /locus_tag="JNZ73_000135"
     CDS             31113..31976
                     /locus_tag="JNZ73_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HAY8730556.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRCSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            31991..34006
                     /locus_tag="JNZ73_000136"
     CDS             31991..34006
                     /locus_tag="JNZ73_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAY8730557.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAL
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGCLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            34080..34778
                     /gene="ypfH"
                     /locus_tag="JNZ73_000137"
     CDS             34080..34778
                     /gene="ypfH"
                     /locus_tag="JNZ73_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY8730558.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(34888..35088)
                     /locus_tag="JNZ73_000138"
     CDS             complement(34888..35088)
                     /locus_tag="JNZ73_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HAY8730559.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(35116..36243)
                     /gene="dapE"
                     /locus_tag="JNZ73_000139"
     CDS             complement(35116..36243)
                     /gene="dapE"
                     /locus_tag="JNZ73_000139"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HAY8730560.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIIEQLVA"
     gene            complement(36247..36603)
                     /locus_tag="JNZ73_000140"
     CDS             complement(36247..36603)
                     /locus_tag="JNZ73_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HAY8730561.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLYAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            36984..37043
                     /gene="ypfM"
                     /locus_tag="JNZ73_000141"
     CDS             36984..37043
                     /gene="ypfM"
                     /locus_tag="JNZ73_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HAY8730562.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(37142..40255)
                     /gene="acrD"
                     /locus_tag="JNZ73_000142"
     CDS             complement(37142..40255)
                     /gene="acrD"
                     /locus_tag="JNZ73_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HAY8730563.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNAAINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKAEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFIHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSADFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNSYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGTQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(40457..42180)
                     /gene="narQ"
                     /locus_tag="JNZ73_000143"
                     /pseudo
     CDS             complement(40457..42180)
                     /gene="narQ"
                     /locus_tag="JNZ73_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            42386..44365
                     /gene="aegA"
                     /locus_tag="JNZ73_000144"
     CDS             42386..44365
                     /gene="aegA"
                     /locus_tag="JNZ73_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HAY8730564.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDTPGVYDALPFIIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            44433..45008
                     /gene="nudK"
                     /locus_tag="JNZ73_000145"
     CDS             44433..45008
                     /gene="nudK"
                     /locus_tag="JNZ73_000145"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HAY8730565.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            45134..46165
                     /locus_tag="JNZ73_000146"
     CDS             45134..46165
                     /locus_tag="JNZ73_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HAY8730566.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQSFRLVRYAAEPTCDNW
                     HGPDARPTL"
     gene            complement(46279..48282)
                     /gene="tkt"
                     /locus_tag="JNZ73_000147"
     CDS             complement(46279..48282)
                     /gene="tkt"
                     /locus_tag="JNZ73_000147"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY8730567.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(48302..49252)
                     /gene="tal"
                     /locus_tag="JNZ73_000148"
     CDS             complement(48302..49252)
                     /gene="tal"
                     /locus_tag="JNZ73_000148"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY8730568.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPLDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            49541..51820
                     /gene="maeB"
                     /locus_tag="JNZ73_000149"
     CDS             49541..51820
                     /gene="maeB"
                     /locus_tag="JNZ73_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HAY8730569.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFVADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            52113..52448
                     /gene="eutS"
                     /locus_tag="JNZ73_000150"
     CDS             52113..52448
                     /gene="eutS"
                     /locus_tag="JNZ73_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HAY8730570.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            52461..52940
                     /gene="eutP"
                     /locus_tag="JNZ73_000151"
     CDS             52461..52940
                     /gene="eutP"
                     /locus_tag="JNZ73_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HAY8730571.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            52915..54288
                     /gene="eutA"
                     /locus_tag="JNZ73_000152"
     CDS             52915..54288
                     /gene="eutA"
                     /locus_tag="JNZ73_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HAY8730572.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVSDLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVCATVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            54812..55503
                     /locus_tag="JNZ73_000153"
     CDS             join(54812..55058,55058..55503)
                     /locus_tag="JNZ73_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY8730573.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALPPFNIGM
                     LTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHEK
                     VIGAFIEKHMFY"
     gene            complement(55622..56443)
                     /gene="murI"
                     /locus_tag="JNZ73_000154"
     CDS             complement(55622..56443)
                     /gene="murI"
                     /locus_tag="JNZ73_000154"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY8730574.1"
                     /translation="MRLNMFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNM
                     NAPYGNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEKYSGYAKSFPFPI
                     LSIIEPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLI
                     EEGNLNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAH
                     DQAISIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(56465..57925)
                     /locus_tag="JNZ73_000155"
     CDS             complement(56465..57925)
                     /locus_tag="JNZ73_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8730575.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            complement(57918..58196)
                     /locus_tag="JNZ73_000156"
     CDS             complement(57918..58196)
                     /locus_tag="JNZ73_000156"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8730576.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNIREYKNV"
     gene            complement(58177..58515)
                     /locus_tag="JNZ73_000157"
     CDS             complement(58177..58515)
                     /locus_tag="JNZ73_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY8730577.1"
                     /translation="MLDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMA
                     EPEGRCVRMLSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEP
                     DLRSCANMLA"
     gene            complement(58974..59180)
                     /locus_tag="JNZ73_000158"
     CDS             complement(58974..59180)
                     /locus_tag="JNZ73_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8730578.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            complement(59645..60187)
                     /locus_tag="JNZ73_000159"
     CDS             complement(59645..60187)
                     /locus_tag="JNZ73_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8730579.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            complement(60252..60473)
                     /locus_tag="JNZ73_000160"
     CDS             complement(60252..60473)
                     /locus_tag="JNZ73_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8730580.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLIC"
     gene            complement(60491..60886)
                     /locus_tag="JNZ73_000161"
     CDS             complement(60491..60886)
                     /locus_tag="JNZ73_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY8730581.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLDKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            complement(61646..62398)
                     /locus_tag="JNZ73_000162"
     CDS             complement(61646..62398)
                     /locus_tag="JNZ73_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8730582.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKQK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGYVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCRE"
     gene            complement(63449..64213)
                     /locus_tag="JNZ73_000163"
     CDS             complement(63449..64213)
                     /locus_tag="JNZ73_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY8730583.1"
                     /translation="MRIDQILSPANEEPALANAEPAQEQEQEQEQEQEQEQEQEQEQE
                     QEQEQEQNKAAAIAEFKELQRQQITAQQEECRHPLAMPKPVAKANTDKPTSWFNEEAE
                     EDLRFDSKEEQLPEEREQVETEDQQNDYRNLPAVNNAMAYLFKSGLFHKLKGDELKLY
                     TWMVSKCRYRGNDKYEIWYSRTKMAKALGKSVDTIDRHLEALRKLGLIQEMRQALRGK
                     CGKTYRLRIPSKGTAEEASMTAQQHEREAKVSYLVH"
     gene            complement(64697..65902)
                     /locus_tag="JNZ73_000164"
     CDS             complement(64697..65902)
                     /locus_tag="JNZ73_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY8730584.1"
                     /translation="MSKTPLTAKAIDAAQPKDKQYKLTASLTPGLFLLVHPNGSKYWR
                     FRYWIDKKERLQAIGVYPLISLKEARKRATESRLLIAQGIDPMDLARKEKAIDALNMA
                     AGFKTVAEDWLATRVGGWSESYTKQVKSALEKDVYPVLGKRSIVDITARDVLALLQKK
                     ERTAPEQARKLRQRIGEIFKFAVITELVTRNPVADLDTALKARRPGHNAWIPISEIPA
                     FYKALERAGSVQIQAAIRLLILTALRTAELRLCRWEWINLEDATITLPAEVMKARRPH
                     VVPLSRQAVELLQDQFTRSGYSVFVFPGRFMDKPLSASAILKALERIGYKSIATGHGW
                     RTTFSTALNESGRYNPDWIEIQLAHVPKGIRGVYNQAAYLKMRRQMLQDYADAIDSIL
                     ADGINPLEL"
     gene            66091..67452
                     /locus_tag="JNZ73_000165"
     CDS             66091..67452
                     /locus_tag="JNZ73_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HAY8730585.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAIAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDGCYTNHADSDQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            67473..68360
                     /locus_tag="JNZ73_000166"
     CDS             67473..68360
                     /locus_tag="JNZ73_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HAY8730586.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            68370..69029
                     /gene="eutL"
                     /locus_tag="JNZ73_000167"
     CDS             68370..69029
                     /gene="eutL"
                     /locus_tag="JNZ73_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HAY8730587.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            69042..69503
                     /gene="eutK"
                     /locus_tag="JNZ73_000168"
     CDS             69042..69503
                     /gene="eutK"
                     /locus_tag="JNZ73_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HAY8730588.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGT"
     gene            69588..70712
                     /gene="eutR"
                     /locus_tag="JNZ73_000169"
     CDS             69588..70712
                     /gene="eutR"
                     /locus_tag="JNZ73_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HAY8730589.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITCQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWGGGSYTQSLTLINSLNAASGLPSFG"
     gene            complement(70646..71545)
                     /gene="hemF"
                     /locus_tag="JNZ73_000170"
     CDS             complement(70646..71545)
                     /gene="hemF"
                     /locus_tag="JNZ73_000170"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HAY8730590.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(71549..72418)
                     /gene="amiA"
                     /locus_tag="JNZ73_000171"
     CDS             complement(71549..72418)
                     /gene="amiA"
                     /locus_tag="JNZ73_000171"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HAY8730591.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            72632..73057
                     /locus_tag="JNZ73_000172"
     CDS             72632..73057
                     /locus_tag="JNZ73_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase YpeA"
                     /protein_id="HAY8730592.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            73044..73493
                     /locus_tag="JNZ73_000173"
     CDS             73044..73493
                     /locus_tag="JNZ73_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HAY8730593.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            73554..74129
                     /locus_tag="JNZ73_000174"
     CDS             73554..74129
                     /locus_tag="JNZ73_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HAY8730594.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            74225..75124
                     /gene="yfeX"
                     /locus_tag="JNZ73_000175"
     CDS             74225..75124
                     /gene="yfeX"
                     /locus_tag="JNZ73_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HAY8730595.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMVL"
     gene            complement(75304..76728)
                     /gene="murP"
                     /locus_tag="JNZ73_000176"
     CDS             complement(75304..76728)
                     /gene="murP"
                     /locus_tag="JNZ73_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HAY8730596.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAARACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(76732..77628)
                     /gene="murQ"
                     /locus_tag="JNZ73_000177"
     CDS             complement(76732..77628)
                     /gene="murQ"
                     /locus_tag="JNZ73_000177"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAY8730597.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            77591..78592
                     /gene="murR"
                     /locus_tag="JNZ73_000178"
     CDS             77591..78592
                     /gene="murR"
                     /locus_tag="JNZ73_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HAY8730598.1"
                     /translation="MFEPSVIIFSSCIGGDLTIYDQIIADVWNHISRIDRLKGIIEST
                     LAECEERHKNLSGIFAASPGEWKMLYLTKIRNAESEFTGNEQKIADFLRARVSELKSV
                     SSRQMAKQLGISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSIT
                     SDDSLEVIARKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGR
                     DLSFKLMKIGYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQG
                     ATVIAITSLADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            78779..79570
                     /gene="ucpA"
                     /locus_tag="JNZ73_000179"
     CDS             78779..79570
                     /gene="ucpA"
                     /locus_tag="JNZ73_000179"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HAY8730599.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            79874..80890
                     /locus_tag="JNZ73_000180"
     CDS             79874..80890
                     /locus_tag="JNZ73_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8730600.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDEAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            80890..81723
                     /gene="cysT"
                     /locus_tag="JNZ73_000181"
     CDS             80890..81723
                     /gene="cysT"
                     /locus_tag="JNZ73_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HAY8730601.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            81723..82598
                     /gene="cysW"
                     /locus_tag="JNZ73_000182"
     CDS             81723..82598
                     /gene="cysW"
                     /locus_tag="JNZ73_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HAY8730602.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            82588..83685
                     /gene="cysA"
                     /locus_tag="JNZ73_000183"
     CDS             82588..83685
                     /gene="cysA"
                     /locus_tag="JNZ73_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HAY8730603.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            83819..84730
                     /gene="cysM"
                     /locus_tag="JNZ73_000184"
     CDS             83819..84730
                     /gene="cysM"
                     /locus_tag="JNZ73_000184"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HAY8730604.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MHAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(84934..85657)
                     /locus_tag="JNZ73_000185"
                     /pseudo
     CDS             complement(84934..85657)
                     /locus_tag="JNZ73_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(85690..86064)
                     /locus_tag="JNZ73_000186"
     CDS             complement(85690..86064)
                     /locus_tag="JNZ73_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HAY8730605.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            86169..87020
                     /gene="pdxK"
                     /locus_tag="JNZ73_000187"
                     /gene_synonym="thiJ"
     CDS             86169..87020
                     /gene="pdxK"
                     /locus_tag="JNZ73_000187"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HAY8730606.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(87062..87571)
                     /gene="crr"
                     /locus_tag="JNZ73_000188"
     CDS             complement(87062..87571)
                     /gene="crr"
                     /locus_tag="JNZ73_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAY8730607.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(87612..89339)
                     /gene="ptsI"
                     /locus_tag="JNZ73_000189"
     CDS             complement(87612..89339)
                     /gene="ptsI"
                     /locus_tag="JNZ73_000189"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HAY8730608.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVAQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(89384..89641)
                     /gene="ptsH"
                     /locus_tag="JNZ73_000190"
     CDS             complement(89384..89641)
                     /gene="ptsH"
                     /locus_tag="JNZ73_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HAY8730609.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(90025..90996)
                     /gene="cysK"
                     /locus_tag="JNZ73_000191"
     CDS             complement(90025..90996)
                     /gene="cysK"
                     /locus_tag="JNZ73_000191"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAY8730610.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(91181..91942)
                     /gene="cysZ"
                     /locus_tag="JNZ73_000192"
     CDS             complement(91181..91942)
                     /gene="cysZ"
                     /locus_tag="JNZ73_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HAY8730611.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            92172..93059
                     /gene="zipA"
                     /locus_tag="JNZ73_000193"
     CDS             92172..93059
                     /gene="zipA"
                     /locus_tag="JNZ73_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HAY8730612.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPVSPQVAPAPQPVHSAPQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEA
                     VIIMNVAAHHGSELNGELLLNSIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKP
                     GTFDPEMKDFTTPGVTIFMQVPSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRSMMT
                     PQKLREYQDIIREVKDANA"
     gene            93130..95145
                     /gene="ligA"
                     /locus_tag="JNZ73_000194"
     CDS             93130..95145
                     /gene="ligA"
                     /locus_tag="JNZ73_000194"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAY8730613.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            95147..95365
                     /locus_tag="JNZ73_000195"
     CDS             95147..95365
                     /locus_tag="JNZ73_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8730614.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(95362..96360)
                     /locus_tag="JNZ73_000196"
     CDS             complement(95362..96360)
                     /locus_tag="JNZ73_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HAY8730615.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            96450..97376
                     /locus_tag="JNZ73_000197"
     CDS             96450..97376
                     /locus_tag="JNZ73_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8730616.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(97367..97708)
                     /locus_tag="JNZ73_000198"
     CDS             complement(97367..97708)
                     /locus_tag="JNZ73_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flxA-like family protein"
                     /protein_id="HAY8730617.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(97828..97950)
                     /locus_tag="JNZ73_000199"
     ncRNA           complement(97828..97950)
                     /ncRNA_class="other"
                     /locus_tag="JNZ73_000199"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT        23841 a        24717 c        25616 g        23777 t
ORIGIN      
        1 acaagcaaaa actttctcac tggctgtcac ccttgctgga accgggtgaa gatgtttatc
       61 agctttcggg taacgatctc gcgctgcgac tgaatacaga atcgcaccag gagcgcatta
      121 ccgcactgga tagtcatctc aagcaatttc gtttcttttg ggatgggatg ccgatgcaac
      181 cgcagattgg cgtcagttac tgctatgtgc gctcgccagt gaatcatatc tacctgctgc
      241 tgggagagct aaatacggtc gccgaacttt ccatcgtgac caacgccccg gaaaatatgc
      301 agcgttgcgg ggcaatgtat ttgcaacgcg aattgaaaga taaagtcgcg atgatgaatc
      361 gactacagca ggcgctggag cacaaccatt ttttcctgat ggcccagccg attaccggta
      421 tgcgtggtga tgtttaccat gaaattcttc tgcgcatgaa aggtgagaat gatgaactga
      481 tcggccccga tagcttcttg ccggtcgcgc acgaatttgg tttatcgtcg agtatcgaca
      541 tgtgggtcat tgagcatacg ctgcaattta tggctgaaaa cagagcgaag atgcccgctc
      601 accgttttgc tattaatctg tctccaacct cggtatgtca ggctcgtttt cctgttgaag
      661 tcagtcagtt gctggctaaa tatcagattg aagcgtggca acttattttt gaagtcaccg
      721 aaagtaatgc tctgaccaat gttaagcagg cgcaaatcac cttgcagcat cttcaggaat
      781 taggctgcca gattgcgatt gatgatttcg gcaccggcta cgccagctat gcacggctta
      841 aaaatgtgaa tgccgatctg cttaaaattg acggcagctt tatccgcaat attgtgtcaa
      901 atagtctgga ttatcagatt gtggcgtcga tttgccatct ggcgcgaatg aagaaaatgc
      961 gggtagtggc agagtacgtt gaaaacgaag agatccgcga ggcggtgctc tctttgggga
     1021 tcgattatat gcagggttat cttattggta agccgcaacc gttaattgat acgctgaatg
     1081 aaatcgaacc cattcgcgaa agtgcctgaa taatgcgggc cgacatttct cgtcggcccg
     1141 caaagcatta agcggcgatt tctggggtac tttcttcttc aattttcaac cgccagccag
     1201 ccacgccttc ccagtattct tgctcctttt ccagatcaag cagtaccagc gcattctgac
     1261 taaaccagtc atgcgggaaa cgcagtgtcc agtgactgtc atcagtaatc agtgtcaatg
     1321 ttgacggtgt ggtggttgcc tgacgttgat tgtttagtaa tacgccaagg cgcaatagct
     1381 gtatcagtgg caggaactgt ttcttcttaa acaaggtaaa gcgcggcaga tcgtcgagct
     1441 taatcgcttt acggtgatag cgcagcagtg tcgccatcat cagctgctgt tcctgattaa
     1501 aacccggcaa gtcactgttt tgcagaatat aagcggagtg gcgatgcaaa ccgctgtggt
     1561 tgatattcaa cccgacctca tgcagcatgg cggcccatcg cagtagcgcc tccagttgcg
     1621 gatgcgccag cttcggttgc tgttcccgcc actgttcgta catttgcata gtggtatcca
     1681 gcactcgtcg ggcctgttcg ctgtctatgt gatactggtt ggcgaggctg ctggcggtgc
     1741 gactacgcac atcctgatga cggaaacgtc cttccatttc atacagtacg ccttcgcgta
     1801 acgccccgtc agaaaggcgc agttcacgga tggctaaagc atcaaacaca ccacataaaa
     1861 tcgccagtcc aggaacgaag actgttttcc gctcttcgga aagacccggt aaactcagcg
     1921 atgcgaaatt acggtgacgt aaaatttctt ttaccagttt ttccagacgt tccggggtaa
     1981 ttatcccgtc tttctcgccc atttccatta acacttcatg ggcggctttt atggtaccgg
     2041 aagcgcccat tgcaacgttc cagccctgaa tacggaattg ccaggttaaa gtttccagtt
     2101 tttgtgctgc cgccatgcga gcgcgctgaa aattctcttt attgatgacc ccgccaggaa
     2161 aataaagctg ggcaaagctg acacaaccca tccggcggct ttcaacgaga ataggttcga
     2221 aattttcacc aatcaccagt tccgtagatc cgccgccaat atcaataacc agtttgcgac
     2281 ctttttccgg ttgggtatgt tccacgccca taaaaatcag acgggcttct tcattaccgg
     2341 aaataatttc aatcgggtag ggaatgacct tttccgcgcg tttcagaaag tcagtggcgt
     2401 tcagcgcctg acgcagcgta tgggtgccga ctatacagac gctggcagga gaaaaccctt
     2461 gtagccgttc ggcaaacagc gacagacagt tcaaaccgcg cgtcattgcc tcttcactca
     2521 acatattatc tggccccagg ccgtccgcca gatgcacccg ctgtttcagg cggccaataa
     2581 tctgcatggc accatctacc acacgggcta tgaccatgtg aaaactgttt gaaccaagat
     2641 cgaccgcagc aaactcctgc ggacgagggg atttatcgtg tattggcata gggttattca
     2701 ggttgttcga gtgatttgat gtagtcgtaa atcgccaact gcgcccgtac tttgcggcga
     2761 ttgccgcggg gaacgtagcg attactgagt tctttatcga tataacgtgc tttgaccgta
     2821 tcgctgaaca atatgtcgat gatgtccagc acccgctgct tcaggcgcgg atcgagcagc
     2881 ggtgtcgcca cttcaatacg ataatcaata ttgcgcgtca tccagtcggc ggaagaaagg
     2941 tagacctttt tatcgccgcc attttcaaaa atataaaccc ggtcatgttc aaggtaacgg
     3001 tcaacaatac tgatggcacg aatgttgtcg ctaatgcctt ccagattggg gatcagcgaa
     3061 cacattccgc gaaccagcag attaaccggt acgccggagc tggaggccgc atacagacga
     3121 tcaaccaggc ctttatcgac aaggttattt agcttcaggg tgataccact gggcagccct
     3181 tgctgcgcgt tggcgatttc gcggtctacc atttcatata acagacggcg agagttttgc
     3241 ggtgacacca ttaaataatc aaatgtcacc gggcggtatg ggttttcaat aaagttaaat
     3301 acccgccgta cttcgttggt gatacgcgca tctgcggtca gcaacgaata gtcagtataa
     3361 agacgcgcgg ttttttcgtt aaagttcccg gtcccgatgt gtgcgtaacg gaccacttca
     3421 ccgttttctt tacgtgaaat caggaacagt ttggcgtgaa ttttcagccc cggcgcagag
     3481 aagataacgt gcacgcctgc ttcggtcagg cgcttcgccc agtgaatgtt ggcttcttcg
     3541 tcgaaacgcg cctgtaactc aaccaccaca gtgactttct taccgttatg tgcggcgtgg
     3601 atcatcgagt cgatgatgcg tgaatctttc gccacgcggt aaatgttaat tttaatcgcc
     3661 agtacgctcg ggtcgaacga agcctgacgc agcagttcca gcacatgccc aaaggtgtga
     3721 taaggataat agagcaacac atcgcgttcg cgaatggcat caaaaccatt gcggaactgg
     3781 gctttatcaa accaaatatg gcgtaaacgc ggcagtggtt tgttcaccag attggctttg
     3841 ccgacattgg gaaaattaat aaagtcttta aaattatgat aacgaccgcc ggggacgatg
     3901 gagtcgtagc gggaaatagt cagtttttcg cgtaacactt caaccagcgc attgggcata
     3961 tcgcgctgat aaacaaaacg caccggctca gcagttaaac gctgcttgag actggaagac
     4021 atcaactcca tcaggctggc ttccatctca tgcactaaat cgtattcggc atcgcgggtc
     4081 atcttcattg aataggcatt cagcgcgtca taatcaaaga agcctttgaa aatatcatca
     4141 aggcagtaac gcagaatgtt atccagaaga atcatcggct tgcgtcgacg cggcgcttct
     4201 ggcggtaaat tcacaaagcg cggcacttta tctgatggga tctccagcag cgcgtaacgg
     4261 atgctatcgc tacggataat ttccaccgcc agataggtgt aatcatcttt caggaactgc
     4321 actaagtcag tgtcaggatt gattaaaatc ggcgtaatgt gctgacgcag atactgctta
     4381 aaataatgac gcagccagtt ttgttgattg acggagagct ggcgttcatt aatcaggaag
     4441 atctggttgc gcgccatctc cagcaatagc tcgttgtaga ggccgtcgaa ttcctgatcg
     4501 gctttcagca cccgggactg aattttgccc agtaaatggc gggaatgagc gttggagcct
     4561 tgttcttcgc taataatgat gcgtcgcttc agttcagcga agcggacttt atagaactca
     4621 tcaaggttat tggaatagat ccccaggaaa cgcatccttt caatcagcgg gttagatttg
     4681 tccgccgctt cctgaagcac gcgttcattg aacgataacc aactgagctc tttttcgatg
     4741 tatagctttt cctgacccat taccactttt actccgtttt attcacggga cactgcctgg
     4801 atattatggc gagcaatatt ggcagatgtc caactatgcc agaaaagggg gaaaaaaact
     4861 gctggagctt gcgatttaca ggtgaattga aaaacgccag cggcagagct ggcgctttaa
     4921 ctacgggggc attactcgtc ggcagcgtag ctctgcggcg gcagacgttg accatccagc
     4981 cacgcggcgt tttcgtgcat tttcagacga ccatcggcaa accagctaat caccagtgga
     5041 taaatagcgt gttcctgggt ttgcacgcgg gcggtgatgt catcttccgt atcaccagca
     5101 aataccggga ctttcgcctg taaaataacc gggccaccgt ccagttcatc ggtgacgaaa
     5161 tgcaccgatg taccgtgctc ttcatcgcca ttttccagcg cctgacgatg ggtgtgtaat
     5221 ccgggatatt tcggcagcag agaagggtga atgttcagca aacgcccggc atagtgggag
     5281 acaaacgccg ggctgagaat gcgcataaaa ccagccagca cgaccacatc gggtgcgtac
     5341 atgtcgattt catgaatcaa ctcccggtca taggcttcac gactgtcaaa cgcgctggcg
     5401 atgagcgtat gcgttgcaat acccgcctgg cgggcgcgtt caaggccgaa cgcgtcggcc
     5461 ttattgctga aaactgcccg tacggtgcct ttaattttgt tggttttaca ggcgtcaata
     5521 attgcctgta aattacttcc gttgccggaa ataagcacca caatattcat tattcgataa
     5581 ccacgcgttg ttcggaatca gaggctttga tgataccgat tttccacgcg ttttcaccgt
     5641 tggcatttag cagggcgagg gctttgtcca cttccggagc aggcagggcg ataatcatcc
     5701 cgacgccgca gttgaaggtg cgatacattt catggcgctc aacgttacct gccgtttgca
     5761 gccagttgaa cacttccggc cactgccagg aagattcatc aatcactgcc tgggtattat
     5821 ctggcaatac gcgcggaatg ttttcccaga agccgccgcc ggtcaggtgc gcaatggcat
     5881 gcacatcgac cttttcaatc aactccagca ctgacttcac gtaaatgcgg gtcggtgcca
     5941 gcagatgatc ggctaatggc ttaccatcaa gttcggtggt ttgcggatca caaccgctga
     6001 cttcaagaat tttgcgcacc agcgaatagc cgttcgagtg cggaccgctg gaaccgagtg
     6061 caatcagcac atcgccgtcg ctgactttag agccgtcgat gatttctgat ttttctacca
     6121 cgcccacgca gaaacccgcg acatcgtaat cttcaccgtg atacatcccc ggcatttctg
     6181 ccgtttcgcc acccaccaga gaacagcccg attgcagaca accttccgcg atgccgctga
     6241 tcactgctga agcggtatca acatccagtt ttccggttgc gtaatagtcg aggaaaaaca
     6301 gcggttccgc accttgcacc accaggtcat taacgcacat ggcgaccaga tcaataccaa
     6361 tggtgtcgtg acgttttaag tccattgcca gacgcagctt ggtacctacg ccgtcagtgc
     6421 cggaaaccag cacgggttca cgatattttt gcggcaatgc acacagcgca ccgaagccgc
     6481 ccagaccgcc catcacttcc ggacgacgcg ttttcttcac tacgcctttg attcttccaa
     6541 ccagagcatt acccgcgtca atatcaacac cggcatcttt gtagctaaga gaggttttat
     6601 cggtcactgc ttgggtcccc acgcgttact tgcggtagaa aaataaaatt cggcgcaatt
     6661 ctaacaggga aagcaaacgt ttgcgagact gttttacaca acctttttgc acgtcttttc
     6721 cccgggcgcg cggcgaaaga agacttgtgc cagggcaaag gttagttttc ggatggaata
     6781 atcttctttc ataaccattt gaatataaaa taactttatc tcaaactgtt atcattttga
     6841 ctaaagtcaa cgaaaataat attgccgcct tgaagaaagg aggtataatc cttcgatttt
     6901 ttttgtggct gcccctcaaa ggagaaagag tatgaagatc gtggaagtca aacacccact
     6961 cgtcaaacac aagctgggac tgatgcgtga gcaagatatc agcaccaagc gctttcgcga
     7021 actcgcttcc gaagtgggta gcctgctgac ttacgaagcg accgccgacc tcgaaacgga
     7081 aaaagtaact atcgaaggct ggaacggccc ggtagaaatc gaccagatca aaggtaagaa
     7141 aattaccgtt gtgccaattc tgcgtgcggg tcttggtatg atggacggtg tgctggaaaa
     7201 cgttccgagc gcgcgcatca gcgttgtcgg tatgtaccgt aatgaagaaa cgctggagcc
     7261 ggtaccgtac ttccagaaac tggtttctaa catcgatgag cgtatggcgc tgatcgttga
     7321 cccaatgctg gcaaccggtg gttccgttat cgcgaccatc gacctgctga aaaaagcggg
     7381 ctgcagcagc atcaaagttc tggtgctggt cgctgcgcca gaaggtatcg ctgcgctgga
     7441 aaaagcgcac ccggacgtcg aactgtatac tgcatcgatt gaccagggac tgaacgagca
     7501 cggatacatt attccgggcc tcggcgatgc cggtgacaaa atctttggta cgaaataaag
     7561 aataaaaata attaaagccg actttaagag tcggcttttt tttgagtaaa gcgcctataa
     7621 cacataatac agaggataat actatgacgc gccgtgctat cggggtgagt gaaagaccgc
     7681 cacttttaca gacaatcccg cttagtttgc aacatttgtt cgccatgttt ggtgcaaccg
     7741 tcctggtgcc cgtcttattt catattaacc cggcgactgt actgttattt aacggtattg
     7801 gaacgctgct gtatctcttc atctgtaaag ggaaaattcc ggcttatctt ggttccagct
     7861 ttgcctttat ttcaccggta ttgttactgt tgccgttagg gtatgaagtc gcgctgggcg
     7921 gctttattat gtgcggcgtg ctgttctgcc tggtttcttt tatcgtgaag aaagcgggga
     7981 ccggctggtt ggacgtgctg tttccacctg cggcaatggg cgcaatcgtt gccgtcatcg
     8041 gtctggagct ggcgggcgta gctgccggta tggcgggttt actcccggct gaagggcaaa
     8101 cgccaaactc caaaaccatc atcatctcta ttaccaccct ggcggtcacg gttttaggtt
     8161 ccgtgctgtt tcgtggtttc ctggcaatta tcccgatttt aattggcgtg ctggtggggt
     8221 acgcgctctc tttcgcaatg ggaattgtcg ataccacgcc gattattaat gctcactggt
     8281 ttgcactgcc aaccctctat acgccgcgct tcgagtggtt tgccattctg actattctgc
     8341 cagcggcgtt agtggttatt gccgaacacg tagggcacct ggtagtaacg gctaatatcg
     8401 tcaaaaaaga tctgctgcgc gatccaggtc tgcaccgttc gatgtttgct aacggcctgt
     8461 cgaccgtgat ctccggcttc tttggctcta cgccaaatac cacttacgga gaaaacattg
     8521 gcgtgatggc gatcacccgt gtttacagta cctgggttat cggcggggcg gcgattttcg
     8581 ctatcctgct ttcctgcgtc ggtaaactgg ctgccgctat ccagatgatc ccattgccgg
     8641 tgatgggcgg cgtttcgctg ctgctttatg gtgtcattgg tgcttccggt attcgtgttt
     8701 tgatcgaatc gaaagtggat tacaacaaag cacagaacct gatcctgact tccgtgattt
     8761 tgatcatcgg cgtcagtggc gcgaaggtaa acatcggcgc ggcggagtta aaaggtatgg
     8821 cgctggcgac catcgtcggt atcggcttaa gtttgatttt caaactgatc agtgtgctgc
     8881 gccctgaaga agtggtgctg gacgcagaag acgcggatat aacagacaaa taatgtaccg
     8941 accgggcagt gttgctgccc ggttcaaaca tcatgggatt ctgtggtaaa cttctcgcga
     9001 ttttgtgaaa tcctggttga ggtatctctg aacacaccgg cacagctctc tttgccactt
     9061 tatcttcctg acgacgaaac ctttgcaagt ttctggccgg gggataactc ctctttactg
     9121 gccgcgctgc aaaacgtgct gcgtcaggaa catagcggtt acatctatct ctgggcacgc
     9181 gaaggcgcgg ggcgcagcca tctgctgcac gcggcttgcg cggaattgtc gcagcgtggc
     9241 gatgcggtgg gctatgtccc gctggataaa cgcacctggt ttgttccgga agtgctcgac
     9301 ggtatggagc atttgtcgct ggtctgtatc gacaacattg agtgtattgc aggcgatgag
     9361 ttgtgggaga tggcgatttt cgatctctac aatcgaattc tggaatcggg caaaacacgg
     9421 ttgttgatca ccggcgatcg tccaccgcgg cagttgaatc tgggattacc ggatctcgcg
     9481 tcgcgtctcg actgggggca gatctacaaa ttgcagccac tttctgatga agataagttg
     9541 caggcgctac agttacgcgc gcgtttgcgt ggttttgaac tgccggaaga tgtggggcgt
     9601 ttcttgctga agcggctcga cagagaaatg cgcacgctat ttatgacgtt ggatcagttg
     9661 gatcgtgcgt cgattaccgc gcaacgtaag ctgaccattc cgtttgtgaa agaaattctg
     9721 aagttgtaga taaggtgttt attgtcggat gcggcgcgaa cgcctgacct acgcgaagtt
     9781 aatccatagt attcgtaggc cggataagac gtggcaagcg tcgcatccgg caatgtgctt
     9841 caactagcga tggagcacat cttaacccac gatttccagt acctgctccg gtggacgacc
     9901 aatccgcgct ttgccattcg cgaccataat cgggcgctcc atcagcttcg gattatccac
     9961 cattgcctga atcagcgcct cttcgctaag cgaactgtca gccaggttta gttctttata
    10021 aagatcctct ttctggcgca tcaattcacg ggcgctattc atccccagca ttttcagcaa
    10081 atcgcgcagc gttgccgcat cggcgggtgt ctcaaggtag agcaccactt ctggctccac
    10141 gccgttttct ttcagcaggt tcagcgtttc ccggctcttt gaacagcgtg ggttatggta
    10201 gatttttacc tgtttggtca ttgttcttcc tttaatgcga attacatctt ggtataaggc
    10261 ttaaagcgtt cctgcagctg gcgcaactgg tcgatgcgcg catcgtaacg cgcttgttgc
    10321 aggctgccta atttcacctg cgaactggcg ctactcaaca gcgaaatggc ctgatcgagt
    10381 cgtccggcga gcgcataacc ttctgctcgc gcagccagct cctgatcgcg gttatttagc
    10441 gcggcttccg cctgtgccag caaatcccag ccgttgctgt catctttatt attaaaggtg
    10501 tagcgattca gaatattggc cgcttcttgt ggttgaccgc cttgcagata agcgttcgcc
    10561 aggttgagct gcaacaccgg attggtgcgt aaatcgcggg catttttcag gcgattgatt
    10621 gcctcattgg ctttgttttg ctcaagatcg atatcggtag ccagatcgag ataccatgcg
    10681 ttgccaggtt ctgccgccag taacggttgc agcgttttac gcgcctcgtc gtatttattg
    10741 gctttcatcg cctgtaaagc acgaccatat tgcgccgctc gttgctgaag aacgtttcct
    10801 ttcgcccatt catccagcaa attactggtg agctggttac gtccggaatt atacatcccc
    10861 agtgtgcgcg ctttcgccag atagaaatct tccgacgact gcaccaccat cgggcgcatc
    10921 tgattagcac ggttgcgggc atctgccaga cgactttccg gcaacgggtg agtcaataaa
    10981 atttccggcg ggcgcgagga gtaacgcgcc tgatcgagca atttttccag gaaggtcggc
    11041 atcgcctgtg gatcgaatcc cgagcgttgc agcacctgaa taccaatgcg gtccgcttcc
    11101 tgttcatttt gctgggtgaa actgatcatc ccctggcgcg ttcccgccag tgtaccggtc
    11161 agcgccgcca tccccgcctg cggactggcc atcgccagta aaatagaacc taacgcgccg
    11221 acccaggtca gcggcgcgct gcgttgttga tcttccatcg ctcgcgccag gtgacgttgg
    11281 gtgacgtggg agatttcgtg cgccataact gaagccagtt gactttcgtt atcggaataa
    11341 cggaacaggg cagagtgcag caccacgttg ccgccaaaga aagcaaaggc gttaatttcg
    11401 tcgttgttga tcagaaaaaa atgaaacggt gtcttaaccg aattggcatg cgaaaccaga
    11461 cgcatcccca gcgaattaat atattgcgtt aacagcgggt cattaattaa cggcgcgctg
    11521 ccgcgtagct ggcggacata atagtcgccc atctgcattt cctgaccaat ggaaagcgtg
    11581 cttcctgcgg aggttcccat atccggcaag gtgtctgcgc tgtcggcaaa cgccggggct
    11641 acctgaccaa tggtcatagc agcaatgagg gttgcaacca ggtttttttt caactgcctg
    11701 aacataacct ctatcctgta tttctgaaga tagtcatttg accgatactg tgaaatatcg
    11761 ttcccacctc gccgcctttg agtgtaacgg cgaagattag cccactcaaa tgacttttca
    11821 gtttcctgaa tccgtcataa aaagcgaagt cttttttgtg acatttcgat acaattgcgg
    11881 atcgcaatcc cgctcttgag gttcagggaa ggtctttatg ctcgaaatgt tgatgcaatg
    11941 gtatcgccgc cgttttagcg acccggaagc gattgccttg ctggttattt tagttgccgg
    12001 atttggcatt atctttttct ttagtggcct gcttgctccg ttgctggtgg ctattgtgct
    12061 ggcttatttg ctggaatggc caaccgtgcg cctgcaatct attggctgct cccgccgctg
    12121 ggcgacgtcg attgtattgg tggtttttgt cggtatattg ctactgatgg cgttcgtggt
    12181 actgcctatc gcctggcaac agggcatcta cttaatccgc gatatgccgg ggatgctcaa
    12241 taagctttct gactttgccg ccacgttgcc gcgccgctat ccggtgttaa tggacgcggg
    12301 cattattgat gcaatggccg aaaatatgcg cagtcggatg ctgaccatgg gcgattcggt
    12361 ggtgaaaatt tccctcgcct cgctggtcgg tttgctgacc atagccgtct atctggtgct
    12421 ggtgccattg atggtcttct tcctgctgaa agacaaagag cagatgctga acgccgttcg
    12481 ccgggtgctg ccgcgtaacc gtgggctggc aggacaggtg tggaaggaga tgaatcaaca
    12541 aatcaccaac tatatccgcg gcaaagtgct ggagatgatc gtggtgggga tcgccacctg
    12601 gctggggttc ttgctcttcg ggctgaacta ttcgctgctg ctggcagtgc tggtcggctt
    12661 ctcggttctt attccgtaca ttggcgcatt tgtggtgacc attccggtgg ttggcgtggc
    12721 gctattccag tttggcgcag gtactgaatt ctggagctgc ttcgcggtgt atctgattat
    12781 tcaggcgctg gacggcaacc tgctagtacc ggtgttgttc tccgaagcgg ttaacctgca
    12841 tccgctggtg attattttat cggtggtgat cttcggtggt ttgtggggat tctggggcgt
    12901 attcttcgcc attccattgg cgacgctgat caaagccgtg atccacgcct ggcccgatgg
    12961 gcaaatcgcg caagaataat cttcagcccg acgaactgtt tcgccgggct gatttttcag
    13021 gattcctgac gtaaataaat agcccgataa caaatactca ccagcactgc accgccgata
    13081 atattaccca gcatcaccgg gagcagattg gcggtaataa aatggctgac cgtcagtgcc
    13141 ggaaaattgt ctgcgctact gtgcgccagt tgccagaagg aggtaggggc gaaatggcga
    13201 atggcaatgg cgaacggaat cacaaacaaa ttggcgatac agtgctcaaa gccactggcg
    13261 acaaacaggg tgatggacaa aatcatggcg acgattttgt cgcataacga acgtccgcaa
    13321 taactcatcc acagcgccag gcaaaccatc agattgcaca taatgccgag gctgacagat
    13381 tcagtaaatg tatgatgcat tttgccctcg gcgcagtgta aaatcgcgac tccccacatc
    13441 gcgttttcac tcatcaccag cccggaaaac cagattaaca aactgaaaca ggcaatacct
    13501 gccagattac cgcaggccac cagaagtgcg ttaatcagcc aagttcgcca actaataacg
    13561 ccccgacttt ttgccattac cgtcattacc gacgaggtga acaggctggt gccgcaaacc
    13621 gccagcaaaa taaagccaag tgtaaagcaa aggccgccca ccagatgggt taatgcctat
    13681 gaagacgggg catcggcgat aacggaaagg taaaaagtaa atccgatcgc cataaacacg
    13741 ccagcggaca tcgccattaa aaagaccgac actttactgc gggcaatttt atgggcggca
    13801 atccgttcag cgattgccgc ttttctggcg ctcaactgca agtcgaaaga gagtttgttt
    13861 ctcatgcata ctcctgcaaa agcagattac aacacctcgc gaaccgagat tcccagacgc
    13921 tgcattcgtg acagcagcgt ggtgcgcttc atccctaatc gtgtcgccgc gccacggggc
    13981 ccggcaacaa tgccattggt ttctcgcaat acctgaacaa tgcgctgacg ttcttcttcg
    14041 tcattctctg gcgtcgtcgg gtgcagcaac tgcgccattg aactgcgaag cgctgaatct
    14101 tcatttagcg tcggcagtaa acggctttgt cggacattaa gatgtaaatt cagactgtta
    14161 ccgcgagtca gcagtaccgc tcgctcaatc acgttttcca gctcgcgcac gttgcccggc
    14221 cagtcccacg acatcaactg gcgtagcgcc tcggtcggga tggcgtcaat tgaacgattc
    14281 atatggcgcg ccattttttg cgtgaaatgc tttgctaaaa gagggatatc ttccggacgg
    14341 tcgcgcagcg gaggcaattc cagtgggaag acattcaggc gataaaagag atcgctgcga
    14401 aactggcgat cttcaaccat ttgccacaaa tcacggttgg tggcggcaat gactctgaca
    14461 tttaccggga tcgttctact cccgccgaga cgctcaatct cccgttcctg caatacgcgc
    14521 agcagtttag gctgaagttc taacggcaga tcgccaattt catcgagaaa caacgtgccg
    14581 ccatcggcaa tttcaaaacg accacgatgg gtattaatcg caccagtaaa cgcccctttg
    14641 tcatgaccga ataactcact ttccagaaga ctggcgggga tggcagcgca gttgattttg
    14701 accagcggct tgtcgtgtcg cgggctaagt tgatggatcg ctctggcgat cacctctttg
    14761 ccggttccgg tttcaccgca aatcaacacc gtactgtcgc tcttcgccac aatatctacc
    14821 tgctggagca ggtcttccat tgcctggctt tgatagataa tgtcaccgac gcccagattc
    14881 gaaaggagct gctcgctaag ctggtggttt tcttgctgca aactttcctg caaatcggtc
    14941 attctacggc aggcatcggc attgccaacg gcaatagcga tgcgatcggc tatgtgttgt
    15001 agtagctggc agttttcctc actaaagaga gtggaagagg tatgcgccag caacaatgca
    15061 cccggtgtat ggttgccaaa ggtaagcggt ataaggagcg cagattcgca gccgttagat
    15121 gcaagtaaga gtaataacgg gtcgcgttgc cagagaaaca gatcatctgc acggtgcgtc
    15181 aaggtcggtt gtcgggtttg tagcgtttgt gtcagcaata cactgtcgcc aggcatattg
    15241 tgtggcagac acgcacaatg tgaggcagaa agatcgctgc accacaggct gaacttctcg
    15301 ttctttcgat gatcgcccag taccatactg actgaagcca gaccgaaaaa atgatggatc
    15361 tcacgagcga cgtcagcgat cagatcgtcg agatcaagat gtgacagcac tgcattagtg
    15421 atatccacca gtacccgatg atgatcgcga tctttctcca gcaaatgcag cgccacctgc
    15481 tgttcttcat cattgagcca tgattgcaga accggggtga taatgcgtgc cagccagtta
    15541 aacagtgtgc aatcctgggg agtaaaacgg ctgccgtggg tacgaataaa gcgtagctcc
    15601 gccattcaac tactgccgag aaggacggcg tgacgcgaat aatcgaccat ctcgccctga
    15661 tactctgttg ttgccgtcac atcgctgcca tcatcatggt agcggtaata gaagttgctt
    15721 ttgagaacat gcagttcaat aagttgtacc gggctgaacg gcggcggcag cgggatgaag
    15781 gcgcgtaata aagacgcctt gccgtgtttc tgcgctaacc gctccgcgag cattccgttt
    15841 accgcttcaa ttgttattcc ttgcggcggg gcaaacatcg cttcgtctga catagccatc
    15901 aacagtcctt attaatgaga aatccttctc acgcaatttt tctggccatc agatagatag
    15961 ccggttccag cacaatttca tccaacagat gaatgaattg aatgctacgc gcttgccaca
    16021 cagacgtcgc gtcatctgcc agtggcgcga atgcccgggt caacagggcg gtgtggctgg
    16081 cgtcaataac aatctcacca aaagtcatca cccccagcat cttacgtcgg gcttgctcct
    16141 cttcgaccag tgcaagccaa tcttcgtatt ccgtcagtgg gcagcggaag gcgacattca
    16201 gacaatcaat cacgccaacg tgatgaccga tagccagcga gtaatacatt acctgagagc
    16261 tgtgttccgg catctcgtcg ctactggcga caaacttttt tcgcagcgtc cagaaaacaa
    16321 tttccccgca ctcttcagtc atgtaacccc cggagtacgc gaaagagttg ctgaacgata
    16381 ctattgagac gtggatcgtc ggcatcgcgc aaccaggtgt taacccgatt tcctgtagga
    16441 tcgtcgctta aatggcgcag gagccgatcg caaatttctc gcccctgacg atagcccgcc
    16501 agccgccgcg cttctcgctc aatggcgata cgctgtgatg gcgggatctg cggccacaac
    16561 ggttgcatag tcaccccggt gggatcgcga taatccaccg cgtgaatctt ctgttgcagc
    16621 aaaccgagcg ccacggcgaa accgtgaatg gtggcggccg gtgttggcgg gcagccgggg
    16681 atccaaacat caatggggac aatggtgtcg ctgccgcccc agacgctgta gagatcgtgg
    16741 aaaataccgc cgccgacacc gcacgcgccg taggaaacac agattttatg atcgggggca
    16801 gactcatacg cccgaagtgc aggcatacgc atcgcccggg tgactgcgcc agtaaataac
    16861 aaaatatcgg cgtgacgcgg tgatgaaaca accttaatgc caaaacgttc tgcgtcgaat
    16921 acgggggtga tggcagcaaa aatttcgatt tcacaggcgt tgcagccgcc gcaatcgatg
    16981 cgataaacgt aagccgagcg acggatatcc tgtagcaaat gccgcttcat cttttgcgtt
    17041 tgctcgtcca gcgtgatggg ctggctgaca tattgcgtaa gcactggact catagctgct
    17101 ccttagccac cagcggtaca tctgtatcgt cgttgatcag cgtcgcgcgt tgtttgcact
    17161 ccgggcagac gctcgcttgc gcccataaca tttcgcggtt ttgtggagca ttttgttgct
    17221 gtgccaacaa ttcagcagcc agtgcgacgg ttttttgcgg ggcaaacggg cgttcgcaac
    17281 ggctgcaacg ttgtagatgg aacgtcgcgt gggtatagag atcggcttta tgggtgacgg
    17341 tcagttcaaa gttattggta agctggatgg ctctggtcgg gcacacttct tcacaacgtc
    17401 cgcagtaaat acaacgcccc agatagagct gccaggtgcg cgaattttgc tggtcgtcgg
    17461 tctggatagt cagcgcattt gccggacaag cacaggcgca ggcaccgcag gcaatacatt
    17521 ggctgggcat caggtccggt ttcccgcgaa agcctgggct gacctccagt ggcgcgaagg
    17581 gatatttcac cgtcggggtt ccggcgcgca taatagtttt cagtaacttc agcatcttct
    17641 gtccttactt cagcggcgaa cggttacgat caatgccgta gcgttcgatc tctttataca
    17701 gcacggtttt tgactggcgc ttgcgcacat ctaccagcgt cacacggtcg gtacaggagt
    17761 agcagggatc aaggctaccg ataatcagcg gtgcgtcaga aacggtattg ccgcgcagca
    17821 tgtaacgcaa caccggccag ttggcgtagg tggcggcacg acagcgccag cggaacaatt
    17881 tttggttatc accgagcatg ctccagtgca cgtcttcacc gcgtggcgct tcaacaaagc
    17941 ccagcgcgaa tgcgtgaggt ttatagctaa agccttcggt cagcagtggg gtatccggca
    18001 tgttgtcgag ggcaaattcc agcattgcca gcgaatcaaa cgtctctttg acacggacca
    18061 tcacgcggga gaaaacatcg ccgccggtaa aggtaaacag tgtttttgga atattgccgt
    18121 agtcggcgta ggggtgatca aagcgcaaat cacgggcaaa accactgcgc ggatcagcgg
    18181 ccctacaggg ctataatcac gggcgatttg tcggtcgaga atgccaatgc cctgagtgcg
    18241 ttgttccata ttcggcgtag caagcagcat ctctaccagc tccgacacgt cggagcgcat
    18301 ctcgcgcacc agtttcagcg tttgcagacg ttgctctttg agaatatcgc ggcgaacacc
    18361 accaatcaga ttcagaccgt aggttttacg cgacccggtc agcaattccg ccatcgtcat
    18421 cgacttttcc cgcacgcgga aaaattgcat aaagccggta tcaaaaccaa caaaatggca
    18481 ggagaggcca aggttgagca aatgactgtg tagccgctcg acttccagca gaatcgagcg
    18541 aatagtatgt gctcgttgcg gcacctcaat ccccagtgca ttttcaaccg agttggtata
    18601 ggccacactg tgggcaaaac cgcaaatccc acacacgcgg tccgataaga aggtcacttc
    18661 gttgtagccc atccgcgttt ctgccagttt ctccatgccg cgatggacat aaaacaggcg
    18721 gtaatcagca tcgacaattt gctcgccatc cacaaacaag cggaagtgac ccggttcata
    18781 ggaggtgata tgcagcgggc cgacagggat cacccgcgca tcgctgttgc cctcattgat
    18841 aaacgcatac gtttcggaat ctgtcgtcgg ttcagggcgc agtcgataat ccatcgcatc
    18901 tttgcgcagc ggatgcatat cttccggcca gtcatcgggc aacaccaggc gacgctgatc
    18961 cggcaggcca accggaatca gcccgtacat atcgcgaatt tctcgctcgc cccagaccgc
    19021 ggcagggacg cgcggtgtga ctgacggaaa ctcccgactg tcggcatcga ccagtgcctt
    19081 caccacgatc cagcattttt cggccccttc cattgaaagg gcataataaa ccgcgtaatg
    19141 accgttaagt gtccgctcgt cgttgccaaa caggactgga agccagccat catgttgata
    19201 ataaagataa tgtacaacgt caggcagcag attgattttc accgtaatgg tgacctgttc
    19261 aggcgtttgt cgctcttcat ccagcaccgc gccggggaac tgcgttttca gggcggcgag
    19321 aatcgcttcg ccacgatttg acgatgaatt aacgttcact gtgtttctcc gaaacagatg
    19381 cggtgcctga aggcaaaaaa tcatgccagg tgctacgttg tgcaggcagg tcgtgcgtcc
    19441 ctgagaggac tatagtggaa gcgcccgcca ggatcctgat gacaggttgt ggaatatgcg
    19501 ttcccatcgc cagcatcatg actagcagaa tcaccattgg cgaggtggtc aaccagccga
    19561 gatcaccccg gttaacggcc tgcggcggtt tcgccattaa cacccgcgca gccatccgta
    19621 ccaggcccgc gagcaccagc gttaacagca ataacagcag gacgataatc agcaggtgat
    19681 tacgtgccag tcctgcggta acggtcataa attcgctaag aaaaatgttg aagggcggca
    19741 tccctgccag cgccagcgca ccgccgccaa acagcacggc ggtaaatggc atgattttga
    19801 gcatcccaca gacgacgttg agatcgcgcg tgccgtactt gagcagtaca ttgccggaac
    19861 cgcagaacag cagcgttttt gccagactgt ggtttaaggt gtgcagcagc gcggcaaaaa
    19921 ttcccagcgg cccgccaatg cctagcgcca ccgcgaccag ccccatgttc tccacgctgg
    19981 agtacgccag cagacgctta atgtcccgct gtaccagaat gaaaaatgcc gccacggcaa
    20041 ccgacaacat gccgaagatg agcaacaacc ggttggggaa atcgctgccg atggcctggc
    20101 aaataatgat gtaatagcga atcagcacca acagcgcgca gttcagcaat acggcggaga
    20161 gcagggcgct gaccggactc ggcgcttcac tgtgagcatc cggcagccag gcgtgcatgg
    20221 gaaatagccc ggttttggta ccaaagccaa ttagcacaaa cacaaaggcc aacagcatta
    20281 atgttgggtc aagcaaggac gattgcttaa gaacctcgct ccagaatatc gccatttctg
    20341 cctgcggcat aacgctggcg gcgttggcgt ataccagcac ggtaccgaac agaccaaaag
    20401 cgacaccaac agtacaaata atgatgtact tccatgctga ttccagcgat gaacgctgac
    20461 cgtaaatgcc taccagaaac gccgagctta aggtggtggc ttcgatcgcc gcccacatca
    20521 caatcaggtt attgctggta acaaccagca gcatggtgaa aaaaaacaga tggaagaaac
    20581 cgtagtaatc gcacagtgta acgggtgaaa gctcgccgtg tgccacttca tgacgcatgt
    20641 agccaatcga gtaaacaccg gtgagaaagc caatcacacc aagaatggcg aggaacaaac
    20701 cgcccagacc atcaatatga agccacagtc ccgcagcgaa tatttctcct gcatcagcgg
    20761 cagtttggac cacccagagt gccagaatca gcagcagtgt gatccctaag ctatgtaata
    20821 cagtcaccgt acaagtcgca gagagccccc gtttccggca ggcaaaacag agcagcgaaa
    20881 aaagcagcgg cgtgagcagg agtaaagcga acatcacaga ataactcatc tcattatccc
    20941 ttcagcgcgg tcaagttgtt cacgtccagc gtgccgtggg tacgccatat ttttcttgcc
    21001 agtaacacca tcacaatgac ggcgaagatg gcgtcggtag cgatacctat ttccaccagt
    21061 tccggtgctc gccaggcaag aagcgccagc accagatggg agccgttttc catcaggcag
    21121 taaccaaaaa tttgccgcag gatattgcgc tggctgacaa tgcacagcag gccaagcaga
    21181 aaatgaccta acgctaccgc cagcgccggt ttcagcccgg tagccatcgg tagcttcacg
    21241 ggctgaacga caaatgcgca aagcaggaca attaacgccg cgagcagtgc catcattgcc
    21301 ggaccgaata acgctttttc cgggatgttc tggggaatat ttcgtgcagc gtaagtcatg
    21361 attaacggta ccagcagcac tttggtgata aaggcgctgg cggaccagat cagcagttgc
    21421 tctgcggcga acaggcacga gagagtggcg aaaatagaca ccagcaccag cgactggcag
    21481 gcgtaaaatc cgcatgacag gcgatagctt ttgacgctaa tcacaaacag cgatgtcagc
    21541 atcatcagtc ccgccagatt atttacgatc atagaaccgg tcattcatca tctccttact
    21601 gcaaccaggt cgcggcgatt acgctggaaa tcagcgacat cacaatcagt acaatcaaca
    21661 ccaggcgcat tgaccctggc agcggtgcgg catcggcgac ggcctcgctc ggtttgcccg
    21721 gcacgacgcg accaaaccag cgaataaacc aggcgaaact ggcgaccgat tcaatcatca
    21781 gcagaatcat ggcgggcagc aggatccagt actccactga caacgcaaaa ccggcggcaa
    21841 acagcgggaa tttactgaag aagccgttga acggcggcac accggtaatt gccagcgctg
    21901 ccacgcagaa acccacgcct ggcaacggca gggtgtgcaa tacgccacgc agacgcggca
    21961 acaagcgcgt gccgcagctg taactcagcg cacctgctac aaggaaaaac aggcttttag
    22021 cgaacgcgtg gttgacgatg taggcgatgc taccctccag cgccagccgc gagccgaaga
    22081 tggagagcga caagccgaag aacatccagc caagttgagt gatggtcgac caggccagca
    22141 accgcttcat atcctgctgt ggcaaataca tcagaaagcc ataaagaatg gtgaccagtg
    22201 ccatgaccat gccaacgccg ccaatcacat gcgggatatt gccgccgtcg ataatggcgc
    22261 gggcaaaaat gtaaacgccc actttcacca tcgatgcggc gtggagatag tcgctgatcg
    22321 gtgttggcgc ttccattgcg tccggtagcc acgcttgcat cggtagctgg gccgatttcc
    22381 cccacgcggc aaacaggatg ccgccataaa ccagataacg tgcgtcgcca tgtaactcgc
    22441 tcatcgcgct aagcacaaac gttccggtct gcaaaaacag cgtggcggcg gcaagataca
    22501 accccaacga accgatatgg gtgataagca acgcttttag tgctgaacgc tgcgctttat
    22561 cgctctggta ataactgatc aacgcccagg agcagccgcc cgtaatttca aaaaacaaca
    22621 actgaccgag cagcgtcgag gagagtacca gtcccgccat cgcgccgata aacaccagca
    22681 aaaatgcgta ataacgattc gtgccgttat gcgggtgttc gcgattttta tccgtcagat
    22741 aacccgtgga gtagatcgtg accagcaaac cgagaaacac caccacaaac agaatcagcg
    22801 tactcacgcg atcaatgacc aggccaaaca atgccacatc ccccacgttg accaacgtca
    22861 acgtgacggc aactttatcg gcctgataaa acgcggagat cagcgacaac atgcacagcg
    22921 tggtcagcgc ggcgaacaaa accccccatt cggcggcccg acgttgtggc gaaaacgaaa
    22981 cgaccagtgc gccgataaaa ggcagcatta acgtcgtcag agcaagattt tccatattcc
    23041 gtcagtgctc cttacagacc ggttaaccag aagacccatg caagcgcagc aaggctgaag
    23101 ccaagccaag ccaggtcaca tggtgaatga gtaaaaaacg cccgcgtgcc agcgtgtttt
    23161 ctgcgattga ggccagtaca aaaatcagca aaaccttgag cagcgtaacg acaagtgaag
    23221 tcagcaggca ggcgagagaa agttcttgcg cgcgcccaaa gggcagaaac agggccacaa
    23281 acagtgctgc catcacgacc tgtttcagcc cctgccccca tttcgctagc gccagcccgg
    23341 caccggaata ttcggtcagc gggccttcct gtaattcctg ttctgcttca gcaacatcaa
    23401 agggaatttt tcccatctca atgaagcagg caaaaccaca ggccagtaac gccagtacgg
    23461 tggttagcgg cgagttccag cccatcgcca gcgtattgct gatcatctcg atatgcgtgg
    23521 aacctgctat cagcgccaat accagcagtg agagaataag cattggttcg accagaatgc
    23581 cgagcgtcaa ctcgcgactg gcaccgactc ccgcaaacgg acttccggta tccagcccgg
    23641 aaagagcaaa gaaaaaacga aacagggcaa gaagatagat aagggtgatc agatcgccgc
    23701 cgcccgcaaa aggggaaacg gtaataaata gtggtaaggc catcgccagc accagcatgc
    23761 tgctgattaa tacccacggc atcaggcgga acatcagacc tgaagatgtc ggcgcaactt
    23821 cctggcgttt aaacagtttg tggatatcgc gataatcctg ccagatcccc ggcccgcggc
    23881 gggagtgcat acgcgcgcgt atctgccggg aaatacccgt aaaaagtggg gttagcatca
    23941 gcagaatcac ggcctgtgct aacgcaaaaa tgaccagata tccgtcgcaa agagtttgtc
    24001 tcatggtgat ttccttagac ggcaatagcg attagcagca caaccagtgc ggcgaccaca
    24061 tacaggcaat agagacgaaa gtcgccgctt tgcagatgct gtatttcttt gaccagccgt
    24121 tgggtggcgc tcacgatggg gcggatcacc cgctcatccc agaagggttc tgtgctctga
    24181 gccctggcgg tgacgtgcgc aagaccttta tttagcctca gcgtagggtc gagctgttgt
    24241 cgtagacgaa ataacgcaga gaagaccaca cgcagcggct gcatcacgcc attgcctgac
    24301 ggagccatcg cgttttccca gccgtagccg catgcccagg catctcctgt gcgacgaaac
    24361 gctgcgcggc gcgaacgaca ccacagccag taaaggcctg gcagcaaagg catcgccagt
    24421 agtaacagca acagtaatga aggagtgacc cgtgtatgaa acgtgccggg tacaagtgct
    24481 attccgctgg cgacagtgac gggaggggta ttggtaaacg catgggcaat atgcattatc
    24541 ttcggtgcca gccaactggc actaagcgca attaatacgc agagtgccgc gagcagtagc
    24601 atggcgacga tcatcgtatt tggcacttcc tgtgcctctt cagcgtgtgt actgcgcggc
    24661 gcaccacaga aagtaatacc gtacattttg acgaagcaca ttactgccag cccaccagtg
    24721 actgccagca ttaccatagc aataggaccc gcaagttgta gcgctacggc ttccacacga
    24781 cttagtgaga acagcgactg ccaggtgtac cattcgctga taaaaccatt cagcggagga
    24841 atggctgata tcgcgaggca accaatcagg catgctgcgg ctgtccacgg catccgtttc
    24901 gccagtgccc ccattttttc catgtcgtgg gtatgcaaac gcgaaataat cgcgtccgcg
    24961 ccgagaaata gcagcccttt gaacagcgca tggttgagca gatgaaacag tgcgccgagc
    25021 agtccaacca cggtgagcag cgggtcgtgc agtgacagac cgtccatcgc cacaccgact
    25081 gccagcaaaa taatgccgac gttttcgacg gtactccagg ccagcagccg tttgatatcc
    25141 tgttccgcca gcgcatatag cacgcccagg agcgcggaga ttgcgccgat cgccatcacc
    25201 agaatgcccc accacagagg caaacccgtt tgcgccagca gatccatcgc tactttcagg
    25261 atgccgaaaa taccgatttt gaccattacg ccagacatca acgccgaagc gtgcgatggt
    25321 gcggcagggt gagcgcgcgg caaccagctg tgcaacggca tcatcccggc tttcgcgcca
    25381 aaaccgaaaa aggccagcag aaacaccgcc gacgccagcc ccggagaaag tgaaagcgtg
    25441 cgaaactggc aaaatcgagg ctgccgcttt cgcgccccat cagcaagaag gcgatcataa
    25501 tcagcaccga tccggcgtgg gcgataaaaa agtagagcat gccagcgttg atcgacgttt
    25561 tatcctgcct ggcaatgacc agaaaccagg aagacagcga catcatttca aacagcacga
    25621 tgaaccaaaa agcgttgtcc atcaccagca gggcaaccat cgatgcgatg aaaagattca
    25681 taaagaagcc catcgccgcc gcgcctttgc cctcgtattc gcgcatataa gtcaatgaat
    25741 agagcgaaca aaccaccacc agcaaggaga taaccagcac cataaatgcc gagaggctat
    25801 ccattcgcag ggttaaatcg gcaaagtcaa aaggggtggc aaaatgggcg actaaagtta
    25861 caccagcatg taattgcgta attccgctga tgatgccaat cacgccgccc accagcgatg
    25921 tgatgccgga aagcttaata gccagtctgt ccagaccgag taaaaacagc gaagccagac
    25981 tggcaaacag atagaggatc agcgaccagg ttaataattg cagggcatcc attagtgttg
    26041 ctcctgagtg agggaaaggg gatcctcccc gccattggca aaccagcttg cggcaaggcg
    26101 ctgtttttct ttcatctgac gttgcaggct atcgtcggtg atcagccgta acgcctgatt
    26161 cgggcaagcg cgaacgcagg ccggaccttc tggcaagaaa tcacacaggt cgcatttcac
    26221 cgcgacggtc tggacacccg gttcccagcg cagcaacgca tgttggggcg gcacgttttc
    26281 ttcgccgtct ttgagtgagc cttcagcctg aaaaacatat tgcgcatggg cattcaccgg
    26341 acggcttcct gaagcgctga ttgcgccaaa tgggcagacc acggcgcaaa gcttgcagcc
    26401 aatacagagg ctttcgttga gttggatcgc atcatccctc tgagagatgg cattgaccgg
    26461 gcagacctgc aggcaagggg cttcctcaca gtgatgacac acgacagggg cagtgattgt
    26521 tgacgtcttc gccagggcca ggcgcgggtg ttgctgtaaa ccttgcgttt tatgcacgtc
    26581 cgaacaggca gcgagacagg tattacatcc tgtacaccac agtggttcgg ccaccacaaa
    26641 gcggttcatg tcggctcctt gaaaatgata tgaaacattg catttcatat tgcataaagg
    26701 gtgccattct gattaactca atctattcaa taagttatgt attaactgga ttctgaacgg
    26761 tacaaaaatg atggtcatta acgttcagca aaaaatggat tcgacacccg tgtcgcaggg
    26821 gaaagcgaga atgtgatctt gatcacgaga agtgaggtat gctggccgca agcgcagcca
    26881 gcacggaatg gagcaaagta atcaggcgtg ttctttcagc cagttcagca caacgtcgtg
    26941 gtgattgctg gttttgaaat cgtcaaagac atgttcgatt ttgccatcag cgtcaatcag
    27001 gaagctgatg cgatgaatac catcgtaggt tttgcccatg aaggactttt caccccagac
    27061 gccgaattgt tcgcacacct ggtggtcctc atcagacagg agcgtaaagt taagcagctc
    27121 tttttccgca aaacgggaga gtttttcggg tttatcggtg ctgataccca gcacatcaac
    27181 gcccgctttt ttcaactcat ccatgttatc gcgtaagccg caggcctgta cggtacagcc
    27241 gggggtcatg gctttcgggt agaaataaac cagaacacgc tgtccctgga agtcggtcaa
    27301 attaacttgt tctccgtctt gatccggcaa gctaaatttc ggtgcgatat caccggcttt
    27361 cagtggattc attacttaac tccatcctgt tcatcatgtt gggaataatt gacgacgtta
    27421 atactgcctt gtgcattgag ttctgtacat agggctttga acgcttgctc aatatttgct
    27481 gcgtccgcag atgcggggct gtgggcggtt atctgaatat gcaactgcgc agcccgttca
    27541 ttttcagcag gttgtgtgcg cgacaccagc tccgcaatgt tcatatgatg cgcgtcgaaa
    27601 agtgctgtga agcgttcaat taaatgcggg gagtctgcaa catcgacctg aacccagaca
    27661 gatgctggca ttggcggacg cggacgcgcc gtcgtgcgct tcatcacgat taaaagatcc
    27721 agttcggcac ctttcaacgg taacgttgat tcaatcagag tgatggcatt ccatgaaccg
    27781 gaaagcagca tgataaacgt gaactcttct cccagcatcg ccaggcgact gtcttcaata
    27841 ttacagccgc aactactgac atgacgggtg atggtgttca caattccagg gcgatcggca
    27901 cccaacgcag tgatcaccag ataatgttgc gatgacagtg tcaaactggt tattccttta
    27961 aggggtgagt tgttcttaag gaaagcataa aaaaatcatg catacaacaa tcagaacggt
    28021 tctgtctgct tgcttttaat gccataccaa acgtaccatt gaggcacttg tttgcacaga
    28081 ggatggccca tgttcacggg aagtattgtc gcgattgtta ctccgatgga tgaaaaaggt
    28141 aatgtctgtc gggctagctt gaaaaaactg attgattatc atgtcgccag cggtacttcg
    28201 gcgatcgttt ctgttggcac cactggcgag tccgctacct taaatcatga cgaacatgct
    28261 gatgtggtga tgatgacgct ggatctggct gatgggcgca ttccggtaat tgccgggacc
    28321 ggcgctaacg ctactgcgga agccattagc ctgacgcagc gcttcaatga cagtggtatc
    28381 gtcggctgcc tgacggtaac cccttactac aatcgtccgt cgcaagaagg tttgtatcag
    28441 catttcaaag ccatcgctga gcatactgac ctgccgcaaa ttctgtataa tgtgccgtcc
    28501 cgtactggct gcgatctgct cccggaaacg gtgggccgtc tggcgaaagt aaaaaatatt
    28561 atcggaatca aagaggcaac agggaactta acgcgtgtaa accagatcaa agagctggtt
    28621 tcagatgatt ttgttctgct gagcggcgat gatgcgagcg cgctggactt catgcaattg
    28681 ggcggtcatg gggttatttc cgttacggct aacgtcgcag cgcgtgatat ggcccagatg
    28741 tgcaaactgg cagcagaagg gcattttgcc gaggcacgcg ttattaatca gcgtctgatg
    28801 ccattacaca acaaactatt tgtcgaaccc aatccaatcc cggtgaaatg ggcatgtaag
    28861 gaactgggtc ttgtggcgac cgatacgctg cgcctgccaa tgacaccaat caccgacagt
    28921 ggtcgtgaga cggtcagagc ggcgcttaag catgccggtt tgctgtaaag tttagggaga
    28981 tttgatggct tactctgttc aaaagtcgcg cctggcaaag gttgcgggtg tttcgcttgt
    29041 tttattactc gctgcctgta gttctgactc acgctataag cgtcaggtca gtggtgatga
    29101 agcctacctg gaagcggcac cgcttgcgga gcttcatgcc ccggctggaa tgattttgcc
    29161 ggtgacctcc ggtgattatg caatcccggt gaccaacggt agtggtgctg tcggtaaggc
    29221 gctggacatt cgtccaccag cccagccgct ggcactggtt tctggcgcgc gtacccagtt
    29281 cacgggcgat accgcttcat tgctggtgga aaatggtcgt ggcaatactc tgtggccgca
    29341 ggtggttagc gtgctgcagg cgaaaaacta caccatcacc caacgtgatg atgctggtca
    29401 gacactgacc accgattggg tacaatggaa ccgtctggac gaagacgagc agtatcgtgg
    29461 tcgttatcaa atctctgtta agccgcaggg ttatcagcag gcggttacgg ttaaactgct
    29521 gaacctggaa caggcgggca aaccggttgc agacgcggct tccatgcagc gttacagcac
    29581 ggagatgatg aacgttattt ccgccggtct ggataaatct gccactgacg ccgcgaacgc
    29641 tgcgcaaaat cgtgcctcca ccactatgga cgtacaaagt gcagctgatg acaccggttt
    29701 accaatgctg gtcgtacgcg ggccgttcaa tgtggtttgg caacgtctgc cagcgtcgct
    29761 ggaaaaagtg ggcatgaaag tgaccgacag cacccgttcg cagggcaaca tggccgtaac
    29821 ttataagccg ctgtctgaca gcgactggca ggaactgggc gcgagcgatc caggcctggc
    29881 atccggtgac tataaactgc aggttggcga tttagataac cgcagcagcc tacagttcat
    29941 cgatccgaaa ggtcatactc tgactcagag tcagaacgac gcgctggtag ctgtcttcca
    30001 ggctgcgttt agcaagtaaa aatacagggc tggaatcatc cggccctttt ttctgatatg
    30061 atacgcaaac gtgtgcgtcg gcaggaaaac gcgattttag cggtaattcg cacgaaattt
    30121 gtttgtcgga cgtagttcgg ataaggcgtt cacgccgcat ccgaaaaaac atccggcaca
    30181 ccagacagca aaagatttta aaacgttaat tcacacccag gagtgataaa gatgcaaaag
    30241 caagctgagt tgtatcgtgg taaagcgaaa accgtataca gcacggaaaa cccggacctg
    30301 ttggtgctcg aattccgcaa tgatacgtca gcaggggatg gcgcgcgcat tgagcagttt
    30361 gatcgcaaag gtatggtgaa caacaagttc aactacttca ttatgagcaa actggctgaa
    30421 gcgggtatcc cgactcaaat ggagcgtctg ctctccgata ccgaatgtct ggtgaaaaag
    30481 ctggatatgg tgccggttga gtgtgtcgtg cgtaaccgtg ctgctggctc tctggtgaaa
    30541 cgtcttggaa tcgaagaagg tattgagctg aacccgccgc tgttcgatct gttcctgaaa
    30601 aacgatgcca tgcacgatcc gatggtcaac gaatcttact gcgaaacctt tggctgggtg
    30661 agcaaagaga acctggcacg tatgaaagag ctgacctaca aagcgaacga cgtgctgaaa
    30721 aaactgttcg atgatgctgg tctgattctg gtcgacttca agctggagtt tggtctgtac
    30781 aaaggtgaag tggtactggg cgatgagttc tccccggacg gcagccgcct gtgggacaaa
    30841 gaaacactgg agaaaatgga caaagaccgt ttccgccaga gcctcggtgg cctgatcgaa
    30901 gcctatgaag ccgtcgcccg ccgcctgggt gtacagctgg actgattttt ctgttcatca
    30961 tcttgccgtg cagctggcac ggcaagacaa ccgctctcgg aatatatgca ttgaacgctc
    31021 attcctccgc catttcatcc cgctttcctg tggtaatctt cctctgaacc acctacgatc
    31081 atttctataa tgaatatatt gttgaggtat ctatgcgttg gcaagggcga cgtgaaagtg
    31141 acaatgttga agacaggcgc aacagctctg gcggtccgtc aatgggcggt cccggtttcc
    31201 gtctgccgag cggtaaaggc gggctgattt tactgatagt cgtgctggtt gcaggctact
    31261 atggcgttga tttaaccggg ttgatgaccg ggcagccggt ttctcaacaa caatcaacgc
    31321 ggtcaattag ccctaatgaa gacgaagccg caaaattcac ctcggtgatt ctggcaacca
    31381 cggaagacac ctggggacaa cagttcgaaa agatgggtaa gacctatcag caaccgaagc
    31441 tggtcatgta ccgtggaatg acgcgcaccg gctgcggggc gggccagtcc attatggggc
    31501 cgttctattg cccggcggat ggcacagttt atatcgatct ctccttctat gatgacatga
    31561 aagacaaact tggcgcggat ggcgattttg cccaggggta cgttatcgcc catgaagtcg
    31621 gtcatcatgt gcagaaactg ttaggcatcg agccgaaagt tcgtcaactg caacaaaacg
    31681 cgacgcaggc ggaagtaaac cgcttatctg tgcgtatgga actccaggcc gactgttttg
    31741 ccggtgtctg ggggcatagt atgcagcagc aaggcgttct ggaaactggc gatctggaag
    31801 aggcgctgaa tgcggcgcag gccatcggcg atgaccgttt acaacagcaa agtcaggggc
    31861 gagtagtacc agacagtttc acacatggca cttctcagca acgctgcagc tggtttaaac
    31921 gtggtttcga cagcggcgat ccggcacaat gcaatacttt tggtaaaagc atttaatttc
    31981 cggggcaggg atggctgaac tgactgcgct ttacacatta acagcgcaaa tgaaacgtga
    32041 agggatctgc cgcttgctgg tgttgagcgg agaagagggt tggtgttttg atcatgctct
    32101 taagttgcgt gatgccttac ctggcgactg gctgtggatt tcgccgcagc tagatgctga
    32161 aaaccactgt tcaccctcgg cgctacaaac tttacttggg cgcgagttcc ggcatgcggt
    32221 attcgacgcc cgccacggct ttgatgccgc tgcctttgca gcacttagcg gaacgttgaa
    32281 agcgggaagc tggctggttt tgttactccc tgtatgggaa gagtgggaaa accaacctga
    32341 tgccgactcg ctgcgctgga gtgattgccc tgaccctatt gcgacgccgc attttgtcca
    32401 gcatctcaaa cgcgtactta cggcggataa cgacgctatc ctctggcggc aaaaccagcc
    32461 gttctcgttg gcgcatttta ctccccgtac tgactggcac cccgcgaccg gcgcaccaca
    32521 accagaacaa cagcaactct tacagcagct actgaccatg ccgccgggcg tggcagcggt
    32581 aacggctgcg cgtgggcgcg gtaagtcggc gttggcaggg caactcattt ctcgtattgc
    32641 gggcagtgcg attgtcaccg cgcccgcaaa agcggcaacg gatgtactgg cacaatttgc
    32701 gggcgagaag tttcgcttta ttgcaccgga tgccttgtta gccagcgatg agcaagccga
    32761 ctggctggtg gtcgacgaag ccgcagccat acctgcgccg ttgttgcatc aactggtatc
    32821 gcgttttcct cgaacgttgt taaccactac ggtgcagggc tacgaaggta ccggacgtgg
    32881 ttttttgctg aaattttgcg ctcgctttcc gcatttacac cgttttgaac tgcaacagcc
    32941 gatccgctgg gcacagggat gcccgctgga aaaaatggtt agtgaggcac tggtttttaa
    33001 cgatgaaaac ttcacccata caccacaagg caatatcgtc atttccgcat ttgaacaaac
    33061 gttatggcga agcgagccag aaacgccgtt aaaggtttat cagttattgt ctggtgcgca
    33121 ctaccggacc tcgccgctgg atttacgccg gatgatggat gcactagggc aacatttttt
    33181 acaggcggct ggcgaaaacg agattgccgg ggcgttgtgg ctggtggatg agggcggatt
    33241 atctcaagaa ctcagtcagg cggtatgggc aggttttcgt cgcccgcggg gtaatctggt
    33301 ggcccagtcg ctggcggcgc acggcagcaa tccactggcg gcgacattgc gcggaaggcg
    33361 ggttagccgg atagcagttc atccggctcg tcagcgggaa ggcacagggc ggcaacttat
    33421 tgctggagct ttgcaatata cgcatgacct cgactatctt tcggtgagtt ttggttacac
    33481 tggggagtta tggcgtttct ggcaacgctg cggttttgtg ctggtgcgga tgggtaatca
    33541 tcgggaagcc agcagcggtt gctatacggc gatggcgctg ttaccgatga gtgatgcggg
    33601 taaacagctg gctgaacgtg agcattaccg tttacgtcgc gatgcgcaag ctctcgcgca
    33661 gtggaatggc gaaatgcttc ctgttgatcc actaaacgat gccatccttt ctgacgacga
    33721 ctggcttgaa ctggccggtt ttgctttcgc tcatcgtccg ctattaacat cgttaggttg
    33781 cttattgcgt ctgctacaaa ccagtgaact ggcattaccg gcgctgcgtg ggtgtttaca
    33841 gaaaaacgcc agtgacgcgc agttatgtac cacacttaaa ctttcaggcc gcaagatgtt
    33901 actggttcgc cagcgggaag aggccgcgca ggcgctgttc gcactaaatg aggttcgcac
    33961 tgagcgtctg cgcgatcgca taacgcaatg gcaatttttt cactgactcc ttcaattttc
    34021 tcccatggtc attgtgagtc agcggcgtaa aattaatgac atcagttaag gagttcgcca
    34081 tgaaacatga ccattttgtt gttcaaagcc cggataaacc tgcacaacag ttgttactgc
    34141 ttttccacgg tgtcggggat aacccggtgg caatggggga aataggcagc tggtttgcac
    34201 cgctgtttcc tgatgcgctg gtggtgagtg tcggtggcgc ggagccgagc ggtaatccgg
    34261 cggggcgtca gtggttttca gtgcagggta ttacggaaga taatcgccag gcgcgtgtgg
    34321 atgccatcat gccgacgttt attgaaacgg tacgctactg gcagaaacag agcggagtag
    34381 gggctaacgc cacggcgcta attggtttct cacaaggcgc gattatggcg ctggagagca
    34441 ttaaagccga accgggcctt gcttcgcgcg tcattgcctt taacggacgc tatgcgagcc
    34501 tgccggagac cgcgtctaca gccacgacaa ttcacttgat tcatggtggt gaagatccgg
    34561 ttatcgatct ggcgcatgca gttgcggcgc aggaagcgtt aatcagtgcc ggtggtgacg
    34621 tgacgctgga tatcgtggag gatttaggcc atgcgatcga caatcgcagt atgcaatttg
    34681 ccctggatca cttgcgttat accattccga aacattattt cgaccaagcg ctaagtggcg
    34741 gtaagcctgg cgatgatgat gtgattgaaa tgatgtaaaa gacagattac ctggcctgat
    34801 aagacgcgtg agcgtcgcat caggcatcgt tcaccattgc cggatgcggc gttaacgcct
    34861 tatccggcct acggttgagc actcagctta cttcttcggt tgatcctttt tcggccagtc
    34921 gtcgtcatcg tcccacttat cgttgaaatc acgatgcggg ggaagctccg gcttgttggc
    34981 gagaaatttc ttatggtcga cgcgcttgag atctttaatc acgttcagca ggacgccgac
    35041 caaaaagaca atcaccagaa tccaccaata tttagccagc cagtccatgc ttatttcctc
    35101 ttgcagaacc actcatcacg cgacgagctg ttctatgata cgttgataca tacgggcaag
    35161 tagctgcagg tcggcagcgt tcacacattc attaatttta tgaatagtgg cattgaccgg
    35221 cccgagttcc accacctgcg cccccatgcg agcaataaag cgcccgtcgg acgttccgcc
    35281 tgtggtcagt agctgcggtt taatttcatt atagtgctca accgcgttaa cgaccgcatc
    35341 caccagttta ccgcgcgcgg tcaaaaatgg ctgcccggaa agccaccaat ccaccgtata
    35401 gcgcagttga tgtttttcaa gcagggcaag tacctgcgct ttgatcatct catcagtcag
    35461 ttcggtgctg aagcggaagt taaactgcac aaacagttca cccggaataa cattgttact
    35521 gcccgttccc gcctgaatat tggcaatctg catactggtc gccgggaaga attcattgcc
    35581 ctgatcccac tcaatagcca ctaattcatt aaggaaaggt gctgcgcgat gtaccggatt
    35641 gtcagccaga tgtgggtagg caacatgccc ctgaacgcca tgaatggtga ggttgcaggt
    35701 taacgatccg cgacgaccat ttttcaccac atcacctacc acttcgatac tcgacggttc
    35761 gccgaccagg cagtaatcga gacgctcatt acgtgccatt aacgcctcga cgacttttac
    35821 cgtaccgttg tgggcactgg cttcttcatc agaagtgatc agaaatgcca gtcgccccgt
    35881 atggttggga tgttgtgcga caaaacgttc tgctgctacc accatcgccg ccagcgagcc
    35941 tttcatatct gccgcaccgc gcccgaataa catgccgtca cgaatggtgg gttcaaacgg
    36001 cggattgatc caacgatcgg cgtcgccagg cggcaccacg tcggtatgcc cggcaaaggc
    36061 taacgtttca ccttgcccac gccatgccca aaaattctgc gtatcggcaa aatccatgcg
    36121 ttcaacggta aaaccgatcg cctgcaaacg ttcaatcaac aaagcctgac atcctgcgtc
    36181 atcaggactc agggaagggc ggcgaataag ctgttgtgtc agctcaataa ccgggcacga
    36241 catagactac acctcatgga aaaattgctg ataactggaa tcactgaaac ccagcagcat
    36301 aggcttacca ggcgcgtaga gcaatggacg tttgataatt gcaggcattt cagtcattaa
    36361 tgccgccgca gaggccgcat cggtgatttt attgcgggta gtttcgtcca gtttacgcca
    36421 ggttgtacca cgggtgttga gtaacgcttc ccagcctaat tcgttgataa aaccgttcaa
    36481 taattcgctg tccagcccat cgacgcggta atcatgaaaa cgatagtcga tgttattggc
    36541 ttccagccaa cggcgagcct ttttaatggt gtcacaattt ttgatgccgt aaagtgtaac
    36601 cataatgaat cctttttcga tttatgagaa taaatactca tttaagggtg aaaacatttt
    36661 gcctgcaaat gattatcaga aaatataaca ttatcgaagt attcaaatta acaactatta
    36721 aataatatta aacattttta acgtaaacat taagaatatt catcttgtta tctgtctgta
    36781 ttttaaccag aattaaataa gtattagatg ccgtatgaat atctcgaaat gttgctgtgt
    36841 atcacataca aattatcacc gcaggaccat agtatttaca taaggtttgt cccccggagg
    36901 tagtcatcgc agcaatcgag taaattagct tcaccacggt atattttttc cgtagaatac
    36961 ccataattat aagagaggtt gttatgattg aacgtgaact ggggaactgg aaagacttta
    37021 tcgaagttat gcttcgtaag taattcctga gacaaagtat aaacagcaag aacccgctga
    37081 gcaggttctt aatcggaaag ttgaaaaagg cgacacattg gcatgtcgcc ttttttattg
    37141 cttattccgg gcgcggcttc agcgggaagc ggcggcgcac cagcacaaag aacagcggca
    37201 cgaagtaaat agccagaata gtagctgaaa tcatcccgcc cattacgcca gtacccaccg
    37261 catgctgacc accggaaccg gcacccgtgc tggttgccat tggcaatacg ccgaagataa
    37321 atgccagcga ggtcatcaga atcgggcgta aacgctgacg gcaggcgtgg agcgtcgctt
    37381 caaacaggtc gtggcctttt tggttcatct cgttagcaaa ctcgacgatc aggatggcgt
    37441 ttttcgccga taaaccaatg accgttaaca ggcccacctg gaagtaaacg tcgttttcca
    37501 gcccgcgcat ccaggttgcc agcagcgcgc cgattacccc cagcggcacg accagcatta
    37561 ccgagaacgg taccgaccag ctttcataca atgccgccag acacaggaat accaccagca
    37621 aggaaatggc gtacagcgcc ggagcctgcg tgccggaaag ccgctcctga tacgacatcg
    37681 ccgtccactc cagaccaaag ccgtttggca gctgcttcac taacgattcc ataatatcca
    37741 tcgccgtacc ggtactgacc cccggtgcgg cttccccaac aatctcaacc gcagaataac
    37801 tgttatagcg ttccagacgc ggcgagcctg tttcccagcg tgaggtcgcg aaagcagaga
    37861 agggcaccat gccgccatct ttatttcgga cataccagag attgatgtca tctggcagca
    37921 tgcgatacgg cgcagctgcc tgcacataga ctttcttcac gcgaccgcga tccataaagt
    37981 cattcacata gctcgaaccc caggcggttt gcagtgtgtc gttaatatcg tcgatatcaa
    38041 cgcccagcgc ctgagcttta cgctggtcga tatcaatctg caactgcgga ctgtcatcga
    38101 ggccgttatg gcgcacacgg gttagctccg ggttttccgc cgccagcgcc agcaactgat
    38161 tacgtgctgc catcagcgca tcgtgacccg ctccagcgtg gtcctgcaac tccatatcaa
    38221 aatctgcaga actaccaaga ccgctaattg ctggtgggct gctggcgata acgcgagctt
    38281 ctttaatttg gttaaacgcc ttcgttgcac gctcgataat ggcaaacgag gtgccggtct
    38341 tactgtcgcg ttcgctccag tctttcaggc ggataaacat tctcgccacg ttttgcccgt
    38401 tacccccagg gccagaacca acggtggcaa acaccgacat gatgttgtct ttttcatgga
    38461 tgaagtaata tttctcgatt tgctcaacga ctttcagggt ctgttgttgg gttgaaccgc
    38521 tgggcaattg taccgaggta gtaaacatgc cccggtcttc cagcggtaag aacgacgtcg
    38581 gcaaacgcag gaacaggaac accatgccgc caagcagcag gacataaatc acaatccagc
    38641 gcaggctacg gtggagaatt ttcgccaccc ctttttcgta gcgttcggcg ttgcggttaa
    38701 acatctggtt aaaccaggca aaaaaacctt tttgcccgtg atgctcagct tttttcagtg
    38761 gcttaagtag cgtggcacac agagccggag tgaggatcat cgctaccagt actgacagca
    38821 ccatcgccgc aacaatggta atagagaact ggcgatagat ggcaccggtg gtgccgccga
    38881 agaaggccat tggtacaaat accgccgaaa gcaccatcgc aatcccgacc agtgccccct
    38941 ggatctgccc catcgattta cgtgtggctt cgcgaggagt gagtccttcc tcactcataa
    39001 tacgttcgac gttttccacc accacgatgg cgtcatccac cagcagaccg atcgccagca
    39061 ccatcgcgaa catggttaag gtgttgacgc tgtaaccgaa ggcgtaaagt acggagaagg
    39121 ttcccatcaa caccaccggc acggcgatag tgggtatcag cgtggcgcgg aagttttgca
    39181 ggaacagata cataacgagg aaaaccagag cgatagcttc cagcagcgtt ttcaccacgt
    39241 cttcaatgga ggctttaaca aacgaggtgg tttcatacgc caccttgtat tccagtccgt
    39301 gcgggaaata ctgcgccagc tcgtcgagac gattgagcac cagctccgct gtcgccattt
    39361 cgtttgcgcc ggaggccagt tttaccccca acccggaggc tggcttacca ttgaagcggc
    39421 taagataatc gtatttctcc gcccccattt cgacggtggc gacatcgccc agccttacct
    39481 ctgagccgtc ctgattcacc cgcaaggtga tatcgcggaa ctgttctggt gtttgcagca
    39541 gtgactgggc gttaatggcg gcgttgagcg cctgcttatc gacggaaggt gtaccaccaa
    39601 gttgcccaac cgcaatctgc gcgttctgtg actcaatggc atcagtgaca tctttagccg
    39661 tcatctggaa actgttgagt ttcgccgggt ccagccagat acgcatggaa tattgcgaac
    39721 cataggcatc gatatccccg acgccattca cgcggcttaa cgggtcctga atattactgg
    39781 caacataatc agcaatatcc tgtttatcca tcgagccatc ggtagagacg aaggcaatgg
    39841 tcagaatgtt ggtatcgccg gttttacgca ccgtcacgcc ctgattttgc accgcctgcg
    39901 gtaactttcg catggctgat tgcagctggt tttgtacttg ctgcacggct tcgtccggat
    39961 cggtgcctgc tttaaaactt aaagtgacag atgcctgacc ggtgccactg ctctgagatg
    40021 acatatacat cagattatcg aggccggtca tattttgctc gataacctgg gtcacggtgt
    40081 tttccagcgt ctgggccgat gcgccgggat agttagcggt cactcgcaca ttcggtggcg
    40141 cgagatcggg gtattgttca acgggcaatg aaaaaatcgc cagggtacct gtcagacaca
    40201 acaggattgc cagcacccag gcaaaaatgg ggcgatcaat aaagaaattc gccattaaaa
    40261 gaggacctcg tgtttctgcg tattgttgta ggcttgactt gcttcactgt agcggcaagg
    40321 tacgagccaa tcgtggagaa aaaaaggagt taatgtaaat tatcatgaga ttgctgacac
    40381 actcgcttac aggtcaatat gggcacgttt ttccctctcg cctttaaagg gagagggccg
    40441 gtgtgagggt aattatttac acctcagccg gcgtgttccc cttccccttc ccctcaccct
    40501 ctgcggagcg aaagctaata ctcactaacg tgccgccgcc ggaaggttgt gaaaaagtca
    40561 gcgtcccgcc cagccgttcc gcgcgttcgc gcatgatatt cagtccataa tgaccttcgg
    40621 gttctttcgg ttcaccgata ccaatcccgt tatcacggat ataaaccgtg tgattgccgt
    40681 ccggcgcggt gacgcaactg atggcgattt cgctggcgtt ggcgtgcttc atcgcattca
    40741 gcaccgcttc gcgaataatt tgcaacaaat gcacctgcat ttgcgcatcc agtgccaggg
    40801 ttggcagacg gcagtcgagg gtcagtttag cgctggtttg attttgtaac gtatccagca
    40861 tttccctcaa cgcggagggg agatccgcct gctgcagcgt caggcggaaa gtagtcaaca
    40921 gctcgcgtaa ctgccgataa gcatcattca acgcctggga aaaatcggcc atgatacttt
    40981 gtgcggtggc gttatcttcc ggtatcgaac gcttcagtaa cgtcaactgg atacgtaagt
    41041 aagaaagcac ctgagccagc gagtcgtgca attcgcgggc gatggtcgca cgttcttcca
    41101 tcaacaataa ctgctgaaaa tgcttctgcg cctgattaaa gtacaaaccg cgtcccagca
    41161 tcgatgaaac actgttaagc agcggttctg atgatgaaac gtgactattt tgccagtgca
    41221 gttcgccgta aaccgtctct tgcattgtca ccggtaaaat ctgcattggc tgctcagggt
    41281 ttggcttccc ttcgctaatc cgccagttat ccccaacgtt cagctccaga tattcagctg
    41341 cttcattgtc gcgaacaatc tgcaaaatat ggcggaaaca atgcacatca atctggctgg
    41401 tgttcagcgc ctgggaacac tgcactgata caacacctcc agacgacgct tggcctcgtg
    41461 gagatcgcgg gtcttttctt ctactgacgc ctccagcgaa aggtacaatt tatgcagctc
    41521 gctcgacatt tggttaaagg tttttgcaag cagaccaagc tcattcggca gactggtatc
    41581 cagcggcggc gagtcgaact gcccgtgttc aatacgctga ctggcggtaa ccagctgatt
    41641 cagcggggca accacctgat ggcgtatgcg gcgcaaagta aaaaagacca gcgtgaaaat
    41701 accgataccg ccagccaggg aaatcgccac caccagcagc attttgcgtt cagcgtagtg
    41761 ctgtaaagcc agcacgaaca ggtctatctg attaacataa ttattaatat tggcctgata
    41821 ccacggcaaa tcgcccttgc tgagccgatt attcatctcc agccagttgg cattcagatg
    41881 cgcatagcga gtttttactg cttctggcac ataccagacg ttgaggttgg ttaataccgg
    41941 tgaatgcagt gcctgctgaa acaactggcg atgtgcattg agttgtggac tgccactttg
    42001 caagtcgtag cccaggcggt aactctgcat acgcagcgat ccggcaatat tgatagcctc
    42061 agcgtcgcgc aaactgctcg ccagagttag cagagcgata cccgtggaaa gaatcgacag
    42121 cagcacaatg taaaaaaagg cccgggccag actggccgag acggggcgtt taacaatcac
    42181 acgcgtcttc tccacaaaaa atcattaatg ttccagttcg ctgaaacgca atttagtcag
    42241 tgatgggatt atttgatctg ccacagttta tagcgaaata gttacttatt ctgggcacat
    42301 gaacattgcc ggttcccctg taagtcgggt aataacaaca ctcatataaa gaataaggtt
    42361 tttacaacca aaaaagaagg tcgttatgaa tcgttttatt atggccaaca gtcagcaatg
    42421 tctgggttgt catgcttgtg aaatcgcctg tgtcatggct cacaatgatg agcaacatgt
    42481 cctgagccaa caccattttc atccccgaat tacggttatc aaacatcagc agcaacgtag
    42541 tgcagtgacc tgtcaccatt gtgaagatgc gccctgcgcc cgtagctgcc ctaatggcgc
    42601 aatcagccac gttgatgaca gcattcaggt caatcagcaa aagtgtattg gctgtaaatc
    42661 ctgcgtggtg gcctgtccgt ttggtacgat gcaaatcgtc ctgacacccg tcgcggcagg
    42721 aaaagtaaaa gccacggcgc ataaatgcga cctttgtgca gggcgcgaaa acggtcctgc
    42781 ctgtgtcgag aattgcccgg cggacgcgct gcaactggtc actgacgtcg cactctccgg
    42841 catggcgaaa tcccgccgct tgcgcaccgc acgtcaggag catcaaccgt ggcatgccag
    42901 taccgctgca caagaaatgc cggtaatgag taaagtcgaa caaatgcagg caacgcccgc
    42961 gcgtggcgag ccggataaac tggcgattga agcgcgcaaa accggttttg atgaaattta
    43021 tctgccattt cgcgccgacc aggcacaacg ggaagcctcg cgctgcctta agttcggcga
    43081 gcacagcgtt tgtgaatgga cctgcccgct gcataaccat attccgcagt ggattgaact
    43141 ggtgaaagcc ggaaacatcg acgccgccgt cgagctttct caccagacca acaccctgcc
    43201 ggaaattacc ggacgcgttt gtccgcaaga ccgtttgtgt gaaggtgcct gtactattcg
    43261 cgatgagcac ggcgcggtaa ctatcggcaa cattgaacgc tatatttcag atcaggcgtt
    43321 ggcgaaaggt tggcgtcctg acttaagtca tgtcaccaaa gtggacaagc gggtggcgat
    43381 tatcggggca ggtccggcag ggctggcctg tgcggatgtt ctgacccgca atggcgtggc
    43441 ggtgacggtg tacgatcgcc atccagaaat cggtggcttg ctcactttcg gcattccttc
    43501 tttcaaactg gataaatccc tgctggcacg ccgtcgggaa atcttcagcg cgatggggat
    43561 tcacttcgaa ctcaattgtg aagtgggtaa agatgtctct ttggattcgc ttttggaaca
    43621 atacgacgcg gtcttcgttg gcgtaggcac ttaccgttcc atgaaagcgg gtttacccaa
    43681 tgaagatacg ccgggcgttt atgacgcgct gccgttcatc attgccaaca ctaaacaggt
    43741 gatggggctc gaagagctac cggaagagcc gtttatcaat accgccggac ttaacgtcgt
    43801 ggtactgggc ggcggcgaca ccgcgatgga ctgtgtgcgt actgcgctgc gccacggcgc
    43861 gagtaacgtc acctgcgctt atcgtcgtga tgaagctaac atgccaggct cgaagaaaga
    43921 ggtgaagaac gcccgcgaag agggggccaa cttcgaattt aacgtccagc cggtggcgct
    43981 tgagctgaat gagcaaggtc acgtttgcgg aattcgtttc ctgcgcacgc gtcttggcga
    44041 gccggatgcc caggggcgtc ggcgacctgt accgattgag ggcagtgaat ttgtcatgcc
    44101 agccgatgcg gtgattatgg cgtttggctt caatccacac gggatgccgt ggctggagtc
    44161 gcacggtgta acggtagaca aatggggccg catcatcgcg gatgtggaaa gccagtaccg
    44221 ttaccagacc accaatccga aaatcttcgc cggtggcgac gccgtgcgtg gtgcggatct
    44281 ggtggttacc gcaatggcag aaggacgtca tgcggcacag gggattattg actggctggg
    44341 ggtaaaatca gtcaaatctc actgatagcc tgcgcagaca aacccgactt cacagcgtaa
    44401 tataattgtt catttcgcgc tgtggagtcg gtatgacgca acaaatcacc ctcattaaag
    44461 acaaaattct ctccgataac tatttcaccc tgcacaacat tacttacgat ctcacgcgca
    44521 aagatggtga agttatccgc cataaacgtg aagtttacga tcgcggtaat ggtgcgacga
    44581 tcctcctgta caacgcgaag aaaaagagcg tggttctgat tcgtcagttc cgtgtcgcta
    44641 cctgggttaa tggcaatgaa agcgggcagc tgattgaaac ctgcgccggg ctgctggata
    44701 acgacgaacc ggaagtgtgc attcgtaaag aagcgattga agagacgggc tatgaagtgg
    44761 gcgaagtgcg caaattattt gaactgtata tgtcgcctgg cggtgtgact gagctaatcc
    44821 acttttttat cgccgaatac agtgacaatc agcgcgctaa cgccggtggc ggtgttgaag
    44881 atgaagatat tgaagtgctc gagctgccgt tcagccaggc gctggagatg atcaaaaccg
    44941 gcgagatacg tgacggtaag acggtgttat tgcttaacta tttgcaaatg tcacatttaa
    45001 tggactgaaa aatagcaata atatttcgtt gtttattatt ggctcaatcc gacaaatccg
    45061 attgagccgc gctacctgcg caacgaagat acgacttgtg ctgtttgttt gaacttctgg
    45121 ggtcgtaccg tccatgcgct atcgcatttt ccttctcttt tttttcgctt tgttgccgac
    45181 gtctttggtg tgggcggcac cagcgcaacg ggcgttttcc gactggcagg tcacctgtaa
    45241 taatcaaaat ttttgtgtgg cgcgcaatac gggcgatcat aatggactgg tgatgaccct
    45301 gagccgcagc gctggggcgc ataccgatgc cgttttacgt attgagcgcg gtgggttgaa
    45361 gtcgccggat gcgtctgaag gggagatagc gccacggctg ctattagatg gtgagccgtt
    45421 ggcattaagt ggtgacaagt ggcggatttc accatggtta ttagtaaccg atgatacggc
    45481 aaccatcacc gcgtttttgc agataattca ggaagggaag gcgatcacct tacgcgacgg
    45541 caatcagacc atttctctga gcggcttaaa agcggcgttg ttgtttattg atgctcagca
    45601 aaagcgcgtt ggcagtgaaa ccgcgtggat caagaaaggg gacgaaccgc ctctcagcgt
    45661 accgcccgcg cctgcgctga aagaggtcgc ggtggttaac ccaacgccga cgccactctc
    45721 actcgaagaa cgtaacgatt tgctggatta tggaaactgg cggatgaatg gtctgcgctg
    45781 ctcgcttgat ccgttacgtc gtgaggtgaa tgtcactgcg ctgaccgatg ataaagcact
    45841 gatgatgatt agctgtgagg caggggccta taacaccatt gatttggcat ggattgtgtc
    45901 gcgtaaaaag ccgctagcct cgcgcccggt tcggttgcgt ttgccgttca acaacggtca
    45961 ggagacgaat gaactggaac tgatgaacgc aacatttgat gagaaatcgc gtgaactggt
    46021 gaccttagcg aaagggcgcg gattaagcga ttgtggcatt caggcgcgct ggcgctttga
    46081 cggtcagagt tttcgcctgg tgcgttacgc cgcagaacct acctgcgata actggcatgg
    46141 gccagacgcc aggcccacgt tgtgatcacc cgttaaacaa aatcgcagat gcggccctaa
    46201 aaagcgcacg gaccgtcccc tcgcccttct ggggagaggg ttagggtgag gggaacatac
    46261 agcatccggc gatcaccatc aggcaccttt cactcccagc accttatgcg cttttgccac
    46321 aatattctcg gcggtaaagc caaagaacgg gaacagctta tccgccggag cagattcccc
    46381 gtaacccgtc atcccgacaa ttgccccttt cagaccaaca tacttgtacc agtaatcggc
    46441 aatacctgct tccaccgcca cgcgagccgc aacgttagaa ggcaacaccg actcccgata
    46501 ttcctcatcc tgggcgtcga aaatatcggt cgagggcagg gaaactacgc gtacattgcg
    46561 accttctcct gctaatttct ctgccgcttg cagggtaatt tccatctctg aaccggtggc
    46621 aatcagaata atatctggct taccgccgct gtctttcagc acataaccgc cacgagcaat
    46681 ctctttaacc tgatccggcg tacgttccac ctgggccaga ttctgccttg agaggatcag
    46741 tgccgtcggt ccgttgtggc gctcaaccgc cagcttccag cccaccgccg cttccacctg
    46801 atcgcacggt cgccaggtgc tgaaatttgg cgttaagcgc aggctggcca gttgctcaac
    46861 agcctggtgc gtcggaccat cttcgcccag gccaattgag tcgtgggtat aaaccataat
    46921 ctgccgcgct ttcatcagtg ccgccatccg cgcggcgtta cgggcgtatt caacaaacat
    46981 caggaacgtc gcggtatacg gcacaaagcc gccgtggtgc gcgatgccgt tggcgatagc
    47041 ggtcatgcca aattcacgca ccccgtagtg aatgtagttg cccgccggat cttccttcag
    47101 cgaaacagaa cctttccaga tggtcaggtt gctgggagcc agatccgccg aaccgccgag
    47161 caactcaggc agcatcggcc cgtaagcgtt aagcgtattt tgcgaagcct tacgggtagc
    47221 gattttcgcc ggatttgcct gtaactcatt gatatatttc tgagtcgttt tctcccagtc
    47281 cttcggtaaa ccaccgctca tccgtcgggt aaactcttct gccagttgcg gatgagcctt
    47341 tttataggcg gcaaacttct cattccagct ctgctgcgct ttttcgcctt tttcacgggc
    47401 atcccaggcg tgataaatct ctttagggat ctcaaatggc ggatggtgcc agcccagttt
    47461 ttgccgtgcc agcgccactt cttcttcccc cagtggtgcg ccgtgcgcct cttccttacc
    47521 tgctttattc ggcgaaccaa agccaatcac cgtacggcag ataatcagcg acggcttatc
    47581 tttcacgctt tgcgcttcaa ggatcgcttc cttcaccgcc tgcggatcgt gaccgtcgat
    47641 ttcatggatc acatgccagt gataggcttc aaaacgtttt gccgtatcgt cggtaaacca
    47701 gccttctgtt tcaccgtcga tggaaatacc gttgtgatcg taaaaaccaa tcagcttgcc
    47761 tagtcccagc gtgcctgcca gcgaacagac ttcgtgggaa ataccttcca tcaggcagcc
    47821 gtcgcccata aacacatagg tgaagtgatc gacgatctca tggtctggct ggttaaactg
    47881 cgccgccagt gtacgctctg ctatcgccag cccgacggcg ttcgccaaac cttgtccaag
    47941 cgggccggtg gtggtttcaa cgcctggcgt atagccaatc tccgggtggc ctggggtttt
    48001 cgaatgcaac tgacggaagt tcttcagttc ttccagcggc aggtcgtaac cggtcagatg
    48061 tagcaaactg tagagcagca tcgacgcgtg accgttggaa agaataaagc ggtcgcgatc
    48121 ataccaggtt gggtcggtag gattatgttt aagaaaatcg ttccacagca cttcggcaat
    48181 atcagccatg cccatcggcg cgccgggatg accagaattg gctttttgta ccgcatccat
    48241 actgagtgcg cgaatcgcat tggcaaggtc ttttcgggac atataacact ccgtggctgg
    48301 tttatagttt ggcggcaaga agatcttcca gtttgcgttg atcaacagcg aacagacgaa
    48361 tgccttcaga cagtttttct accgccatcg catcctgatt gtgctcccaa cggaactccg
    48421 cttcgctcat gggtgctggg cgtgggaacg tctgagaagg cgggattaat ttacgtacca
    48481 ctggagaaac tttttcctgc agctccttca gcaaattcgg tgcgatagtc agtcgatcgc
    48541 agccggttaa ggcgaggatt tgttcggtgc gacggaagct cgcgcccatc acaatggttt
    48601 catagtggtg ttgcttatag tagtcgtaga tattgcgcac cgatttaacg cccggatctt
    48661 cttccaccac atacgggtcc atcggcttgc gtgcctgata ccagtcataa atacgcccta
    48721 caaacggcga aatcagaaaa acgcctgctt ccgcacaggc ccgcgcctgg gcaaaagaaa
    48781 acagcagcgt caggttgcag ttaatacctt ctttttccag ctcttcggcc gcgcgaattc
    48841 cttcccaggt cgaagccagt ttgatcaaaa tgcgtgattt ctcaacgcct tgttgctgat
    48901 acaagtccac cagatggcgc gccttctcaa tactcttttc tttatcaaaa gagaggcgtg
    48961 catcaacttc tgttgacacg caaccgggta cgattttgag aatttcagca ccgaaattga
    49021 ccgccagttt gtcacacgct gcgaccacct gttgttcctg ggtcttgcca ttttttttac
    49081 cccaggcgat agcatcgtct attaaatgct catattgtga taatccggca gccttgagta
    49141 acagcgaagg attggtggtg gcatccaggg gatgataatg gcgaatggac tcaatatcgc
    49201 cgctgtctgc cacgacagtg gtgaactgtt tgatgccgtc taactcgttc ataggaaata
    49261 ctccttgaaa agtaaagtgt tagatgagtg cgttaattca cacttctgag aaatttcgct
    49321 aaacgcatca aaaaagcata gcagacaggc atggtattgc tggattaagc aggtaatatc
    49381 agtgttatag gattgttacc aaaacattat attaatggtg agagtttgga cttgctcaaa
    49441 gtctgtagac tccggcaggg taataatgtg cgccacgttg tgggcagggg ctgttgccca
    49501 cacactttat ttgtgaacgt tacgtgaaag gaacaaccaa atggatgacc agttaaaaca
    49561 aagtgcactt gatttccatg aatttccagt tccagggaaa atccaggttt ctccaaccaa
    49621 gcctctggca acgcagcgcg atctggcgct ggcctactca ccaggcgttg ccgcaccttg
    49681 tcttgaaatc gaaaaagacc cgctaaaagc ctacaaatat accgcgcgcg gtaacctggt
    49741 tgcggtgatc tctaacggta cggcggtgct ggggttaggc aacattggcg cgctggcagg
    49801 caaaccggtg atggaaggca agggcgttct atttaagaaa ttcgctggga ttgatgtatt
    49861 tgacattgaa gttgatgaac tcgacccgga caaatttatt gaagtcgtcg ccgcgctcga
    49921 accgaccttc ggcggcatca acctcgaaga cattaaagcg ccagaatgtt tctatattga
    49981 acagaaactg cgcgagcgga tgaatattcc ggtattccac gacgatcagc acggcacggc
    50041 aatcatcagc actgccgcca tcctcaacgg cttgcgcgtg gtggagaaaa acatctccga
    50101 cgtgcggatg gtggtttccg gcgcgggtgc cgcagcaatc gcctgtatga acctgctggt
    50161 agcgctgggg ctgcaaaaac ataacatcgt ggtttgcgat tcaaaaggcg ttatctatca
    50221 ggggcgtgag ccaaatatgg cggaaaccaa agccgcatat gcggtggtgg atgacggcaa
    50281 acgtaccctc gatgatgtga ttgaaggtgc ggatattttc ctgggctgtt ccggcccgaa
    50341 agtgctgacc caggaaatgg tgaagaaaat ggctcgggca ccaatgatcc tggcgctggc
    50401 gaacccggaa ccggaaattc tgccgccgct ggcgaaagaa gtgcgtccgg atgccatcat
    50461 ctgtaccggc cgttccgact acccgaacca ggtgaacaac gttctttgct tcccgttcat
    50521 cttccgtggc gcgcttgacg ttggcgcaac cgccatcaac gaagagatga aactggcggc
    50581 ggtacgcgcg attgcagaac tggcccatgc agaacagagt gaagtggtgg cttcagcgta
    50641 tggcgttcag gatctgagct ttggtccgga atacatcatt ccaaaaccgt ttgatccgcg
    50701 cttgatcgtt aagatcgctc ctgcggtcgc aaaagcggcg atggagtcgg gcgtggcgac
    50761 gcgtccgatt gctgatttcg acgtctacat cgacaagctg actgagttcg tttacaaaac
    50821 caacctgttt atgaagccga ttttctccca ggctcgcaaa gcgccgaagc gcgtagttct
    50881 gccggaaggg gaagaggcgc gcgttctgca tgcgactcag gaactggtaa cgctgggact
    50941 ggcgaaaccg atccttatcg gtcgtccgaa cgtgatcgaa atgcgcattc agaaactggg
    51001 cttgcagatc aaagcgggcg ttgattttga aatcgtcaat aacgaatccg atccgcgctt
    51061 taaagagtac tggaccgaat acttccagat catgaagcgt cgcggtgtca ctcaggaaca
    51121 ggcgcagcgt gcgctgatca gtaacccgac agtgatcggc gcgatcatgg ttcagcgtgg
    51181 ggaagccgat gcaatgatct gcggtacggt gggcgattat catgaacatt ttagcgtggt
    51241 gaaaaatgtc tttggttatc gcgatggcgt tcacaccgca ggtgcaatga acgcgctgct
    51301 gctgccgagt ggtaacacct ttgttgccga tacctacgtc aatgacgaac cagacgcaga
    51361 agagctggcg gagatcacct tgatggcggc agaaactgtc cgtcgttttg gtattgagcc
    51421 gcgcgttgct ctgttgtcgc actccaactt tggttcttct gactgcccgt cgtcgagcaa
    51481 aatgcgtcag gcgctggaac tggtcaggga tcgtgcgcca gatctgatga ttgatggtga
    51541 aatgcacggc gatgcagcgc tggtggaagc gattcgcaac gaccgtatgc cggacagccc
    51601 tttgaaaggt tctgccaata ttctggtgat gccgaacatg gaagctgccc gcattagtta
    51661 caacttactg cgcgtttcca gctcggaagg tgtgactgtc ggtccggtgc tgatgggcgt
    51721 ggcgaaaccg gttcacgtgt taacgccgat cgcatcggtg cgtcgtatcg tcaatatggt
    51781 ggcgctggcc gtggttgaag cgcaaaccca accgctgtaa ttcattctta actctcacgc
    51841 ttaccctgaa tattcagggt aagcggtttt cctgaaatat attagcaaag cttattactg
    51901 agtttgcgaa taataaaaaa atcagtatat aaaatatctc gatattattt tatttatatt
    51961 catgcgtcgc atatgaaagt ttattcataa cagtaaatat ctctccattc ttagtgatct
    52021 acctcacctt ttaaacgcgc ttgccgaatt ttgttattta ctctgacgaa aaattgtcac
    52081 gatacacgaa agtttttcac aggcggcgac tcatggataa agaacgcatc attcaggaat
    52141 ttgtgcccgg caaacaggtc acactggcgc atctcatcgc gcatcccggt gaggaactgg
    52201 cgaagaaaat cggcgttccg gatgcgggcg caattggcat tatgacgcta actcccggcg
    52261 aaacggcgat gatcgccggt gatttagcgc tgaaagccgc tgatgtgcat atcggttttc
    52321 tcgaccgctt cagcggtgca ttggtgattt atggctcggt gggcgcagta gaagaagcgt
    52381 tatcacaaac cgtcagcggt ttaggtcgct tattaaatta tacgctttgt gaaatgacca
    52441 aaagttaata cgaggcattc atgaaacgaa ttgcgtttgt gggtagcgtc ggggcaggaa
    52501 aaacaacgtt atttaatgcg ttgcagggga attataccct cgccagaaaa acacaggccg
    52561 tggaatttaa tgataagggc gatattgata cgccgggtga atattttagc catccccgct
    52621 ggtatcacgc cttaattacc acgctacagg atgtagacat gctgatctat gtccatggcg
    52681 caaatgaccc ggaaagtcgg ttgcctgccg ggttgctgga tattggcgtc agcaaacggc
    52741 aaatcgccgt catcagtaaa acggacatgc cagatgctga cgtcgccgca acgcgaaagt
    52801 tattgcttga gacgggtttc gaagagccga ttttcgagct aaacagccac gacccgcaaa
    52861 gcgtgcagca actggtggat tatctggcat ctctcaccaa acaggaggaa gcaggtgaaa
    52921 aaactcatca cagcgaatga tattcgtgaa gcacacgcgc gcggcgaaca ggcaatgtcc
    52981 gtggttctgc gcgccagcat tattaccccg gaagcccgtg aagtgtcgga tctactgggt
    53041 tttaccatca ccgaatgtga tgaatcgatc ccggtaacgg cgtccgtgcc cgccagcgtg
    53101 ccagccgaca aaaccgaaag ccagcgcatc cgcgaaacca tcattgccca gctgccggaa
    53161 ggccagttta ccgaaagcct ggtcgcacag ctgatggaaa aagtgatgaa ggaaaaacag
    53221 tcgctgggcg attttgtcgt cgccagcgcc gggccgcacc tcgaatccgt gatcgccggt
    53281 cacggagccg gggcgcaaac cctttctgaa caacggctgt gtcgggtact gaatatcgat
    53341 atcggcggtg gcaccgcgaa ctacgccctg ttcgatgccg gaaaaatcag cggcactgcc
    53401 tgtctcaacg tcggtggtcg cctgctggaa accgacagcc aggggcgcgt ggtttacgct
    53461 cataaaccgg ggcagatgat tgtggatgag tgcttcggtg caggcactga cgcccgttcg
    53521 ctgaccggcg cgcagctggt gcaggttacc cggcggatgg cggcgctgat tgtcgaagtg
    53581 attgacggaa cgctttcgcc gctcgcgcag gcattgatgc aaaccggttt gctgcccgca
    53641 ggtgttacgc ccgaaatcat tacgctttct ggaggtgtgg gcgaatgtta tcgccaccag
    53701 cccgccgacc cattctgttt tgccgatatt ggcccacttc tggcaacggc gctgcatgac
    53761 catccgcgcc tgcgtgagat gaatgtgcag tttccggcgc aaaccgtatg cgccacggtg
    53821 attggcgcgg gtgcacatac cctttcgctc tctggcagca caatctggct ggagggcgta
    53881 caactgccgc tgcgcaattt gccggtggcg atcccgattg atgaaacgga tctggtgagt
    53941 gcctggcaac aggcgctgct tcagctggat cttgatccca aaactgacgt gtacgtgctg
    54001 gcgcttcccg cctcgctgcc tgtgcgttac gccgcggtac tgacggtcat caacgcgctg
    54061 gtcgatttcg tcgcgcgttt tccgaatccg catcccctgc tggtggtggc cgggcaggac
    54121 tttggtaaag ctctgggcat gttgttgcgc ccacagctac aacaactccc gttggcagtc
    54181 attgacgaag tgattgtccg cgcgggggac tatatcgaca ttggtacgcc tctttttggc
    54241 ggatcggttg tgccggtgac ggtgaaatca ctcgcatttc cttcctgaat acggtagtta
    54301 tcaggtatgg tgcttggtgg ctgggtgtta gtgggattat atccggcagt gtatccggtt
    54361 aaggtgattg gtctggcaag atgattggat gcttttgtgg tgaaaaatgt tttggtgttt
    54421 tagcataacc ctgaagaaag aataaaaata aaaaggaaaa aaccaaaacc agaacagaag
    54481 agaaccggaa agagaagaaa agagaaaatg aaaaataaaa accaaaaaag aaagaatact
    54541 taaatcttcg catgttcttc gggtggtacg caagtttgct agtctgactg cacaataaat
    54601 tttcgcttag aaaataataa gttacataat attctgcttt tttagataaa tataacccga
    54661 aaaaatcatt gttaatctta tgcatttatt atttgattgt aagttgtgat tattataatt
    54721 tagttattgt tagtatgttt tataatgctg gatgggtgat gcctctaatt agttgaatct
    54781 gatgtataat acgggctttt gaggttatct catgaccagc gttaatattc attgtccccg
    54841 ttgtcagtca gctcaggttt accgccatgg tcagaaccct aaaggccgtg acagacttcg
    54901 ctgccgtgac tgccaccgtg tgtttcagtt cacttatact tatcaagcac gtaagccggg
    54961 tatgaaagag ctgataactg aaatggcctt taatggtgcc ggggttcgcg ataccgccag
    55021 gacactgaaa attggtagta acaccgtcat ccggacttta aaaaactcgc gccaaagcga
    55081 ataacttctt cgcctgttgc ccatgctgat gtggcgttta tctgcgagct tgatgagcaa
    55141 tggagctacg ttggcagtaa agcccggcaa cattggctcg ggtacgcgta caacaccaaa
    55201 acaggcggtg tactggccta cacttttggt ccccgaaccg atcaaacgtg ccgggagcta
    55261 ctggcactgc cacccttcaa catcggcatg ctgaccagcg atgactgggg cagctatggc
    55321 cgggaggtgc cgaagaataa gcatctgacc ggcaaaatat tcacccaacg cattgagcgt
    55381 aataatctga cgctacgcac ccgcattaag cggttgggtc gtaaaacaat ctgcttctca
    55441 cgctcagttg agatccacga aaaagtgatc ggagcgttta tcgaaaaaca catgttctac
    55501 taattggatg cactacccag tatgtaactg ggtttgtcca taaaatgcac cttactcagt
    55561 tggtgtccaa cttttggggt gcagttcaga aggacgggat tttacggtgc accggataaa
    55621 tttaaaactg cctgatactg aatgtatgag gtttcgtaat gctcaattcc cctagtaccg
    55681 ttttgtagat ctccatactt ccactagtat ttatttgcaa agttcctata tttgaggagt
    55741 ttaataaatt tagctgtccc aagtgatttt taatagagat agcctgatca tgtgcaggat
    55801 taataaattt tatatccggt gcaactgctt caaaaaaatt ctgaacaatt ggatagtgag
    55861 tacagccgag aataatttca tttactggat ggagggaaat taaatggtta acatgttttt
    55921 tgatgtcatt caagattgca ggagcattaa gattaccttc ctcaattaac attgcaagat
    55981 ttttgcttgg ttctccaaat acatttattg ttgaattcaa tttttttatt agctctttgt
    56041 gacaccccgt tttaatagta aactcggtag cgataatacc aacattttta tattgctggc
    56101 gaatcacatc ttctactgca ggttcaatga tggataagat aggaaacgga aagctcttag
    56161 catatccgct gtatttttcg gattccaaag ttgaggatat agtattacat gcgacagcaa
    56221 ctagtttgac attccttgac tgtaaaaaag tcagcatttt tttagtcaaa ctgatgatat
    56281 catcggcaga acggttgcca taaggtgcat tcatattatc tccacaatag ataatatctt
    56341 caccaggaag caaattaatt atttccttta ccgatgtcaa tccacctaca cctgagtcta
    56401 ttacaccaat tggagattca gcactaaaca tatttaacct catcttttgt agcttatgga
    56461 aaatttaatt actagttctt ttttcccaag aaaatggttc gaaatcagag tttggatctt
    56521 tccagtcaga ttttcggaag gcgtaaatta atattggaat taataaaaat agggcaacta
    56581 atactaacaa ataaagaaca tagctggttg gactaccagt tgggatttgc tcgggcggaa
    56641 taaaacttaa tacaaaagcc agaaaagacc ctaagaatcc aagtgttgcc cagaaaaata
    56701 aagcaggggc tcgatatggg cgttttgtat caggttgaga atgtcgtaga tagatagcac
    56761 tagcgaacat gagcatatac ataactaaat agagcataac tgttaactgt gataaaatct
    56821 gaaaagctgc ttgaactgaa ggcattatca caaaagtaac agaaagaact gagacaatta
    56881 taccttgtac aaacattaga tggtgcccca ttccatgacg attggttttt tggaaaaact
    56941 taggtaaata accagctttc gcgatcgcta atacacctgt attaggccct gcaatccaag
    57001 taaccacccc acccagtacg ccaattgcta gcataaatgc catcacagag ccaagccatg
    57061 gcactccagc ccattcgaac agcatatcgt aggcaaccaa caaactttga gttagactaa
    57121 ttttatctgt aggaataata aaggcgaccc ctaaagtact gagcaggaat attgcaatag
    57181 tacctataga agcgatggta attgcaattg ggtatttttt agttggatta tctaaatcgt
    57241 taacatggac cgcattcatt tccatccctg cataagcaag aaatatgctg gcggcaagaa
    57301 caatattctg gaagttagct atattaggga aaagatcacc ccaacctaat gggatcaccg
    57361 ctggtttacc gctgaaccac agataggaga aaccaagaat aacaaggata atagctggaa
    57421 taattgtccc tataataccg caccacttag atacctgcga gaaaatacca acgccacgta
    57481 atgcaacaca aatagctccc cagtaaacaa atagtacgaa aaataaaata taccatttat
    57541 tactggcaat tgattctgcc acccccatat caacatcaat aaatgcaaca gagactgcac
    57601 caaatgtcag tactgttgga aagtagggga tgacttgtag ccaggtcata agcattccta
    57661 caaaacccca gcgagcccca aatgcttctc ctatccatcg aaatgccccc cctttttgtg
    57721 gatatgttgt agctagctca gccgcaacaa aggcaacagg tactaaaaac ataattgcag
    57781 caaaggtata gtaaaaaatg gcacctaagc cgtattctgc ttcagaaggt ataaatcgta
    57841 gacttactac agcagttata ttcatgatag cgagtgtccc cactgtcatt ttcttacttt
    57901 tgggtgcgat attagcatta gacattttta tactctcgaa tatttttaaa atagaaacat
    57961 ataattacga tgtaaatata aaaactaata ttaactttaa cacttatctt aaaaacacta
    58021 acgcaatctg ctttacaaaa ataagtaata cctaaagtcg taacgacacc aatagtgttt
    58081 tcatcgacat gcttcaaaac ctctttagca ttcattatca ggtgatgatc atcaagtgga
    58141 atttcacaca atttcacatc acagtaacgg cagaatttat gccagcatat ttgcacagga
    58201 tcgcaaatca ggttcggttg atttaccttg tacttcgcgt tttttacacc actgccattt
    58261 taacgccaag cggccgagca tagcagcttc agatgaacca atggttgagc aacctaaggt
    58321 gtgtcgcgaa tcggggaaac tccacgaatc ggaaagcata cgcacacagc gaccttcagg
    58381 ttccgccatt tgtggatatt catctttata aatcatgttt ttatcaatag ctaaattaag
    58441 caactcacgg atttcatcat caagccatgt ttgacagaaa gtagctaagt tttgtcgtga
    58501 atccccatca aggatgagct catcacgtat ggcgctgaat acatttcgag atttcttctc
    58561 atctacacgc aaatgatatt tatgcaatat aaatgaaagc tcaagtgagc tatgaatatc
    58621 gactgcttct ctataacaag aattacctga tttatttttc ataataaacg ccactcaata
    58681 caatcagatt aaacattaca aacacaatct ggattttgca attttaaaac tatcaatttt
    58741 agatatattt taattaatat aaaaccataa acatatcaaa aaattacttt attatggtca
    58801 tggtagatgt gtttcaatgc caacctggtc tattcctaca tatgtgagag catatttaca
    58861 aaaaaatcgt tattgtgatt tcaacggtga tgctgaagtg gcacactaaa tttggccacc
    58921 tgaacagagg tgatatgctc acctcagaac aacatgacgt ggtgttattc tgctcagtac
    58981 tgttctggca atgaatccag ccagtcctct gcatcttcct tgtaacactc gtagatgcag
    59041 taattggtga aactgatgta tttggttagt aactctgctt cgttgttcct gtgcgctctt
    59101 ctggcctgct tacgtagttg tttcgcttct gcccgctggc cttcgagtat ctccagcctg
    59161 tctgcaatat cttgcttcat gttcagctcc tcgcgcgcgc atagtacttg tccgaaaatt
    59221 aatcgcccgt cccatcatta atatcgattc ccacgctgtg taactggcta tgaatgtctc
    59281 tgcgaatgag aattaaacgc ttgatgaact ccatttcctc cgcgctctgg ttgctcggcc
    59341 ccgatttcat gtcgatgatt ttattgatag ttccaagcga atattcagcc cacagacgaa
    59401 gcgtttagcg cttcatggcc tgtttgctca gtggcgaatg tttcaggtct aagacatgtt
    59461 ctgcgagggt cttagcgatg ccctgcatga tgcgtagtgc caacatgatg attttctccg
    59521 gacttgtgct aatatagttc ccgaacattg tttcgagact gcttatcctg tttgtcttgt
    59581 ccgatttgtt cattggtcac attgttaagt cagccggggt aattcccggc tttcttttat
    59641 ccgatcagta ccgaatccct atcgcattga atacgcgttt ccgttgctca gcttcaggat
    59701 cggtggccat ttctggttct ggctccggca tagacggggc ggcatcaaaa tgctcaattt
    59761 cacgaccgaa atacgagttc aggtcaatac ctgccgaact atccactcca tcaaaatagc
    59821 gacgctgttc ctcggtgaga cagctaccca gacgttcctc aacctgcgcc ataaactcaa
    59881 catccggtga gaagtactca tcgggattcg gagcaccgct ctgtttgtgc atctccattt
    59941 tctctgcgtt cgtttttagc ccggcaatgc gtttctccag tccggcggct ttaatagcgt
    60001 cagcaacgat ttgtgggatc tgcggtttaa ttgtttcgat aacttcatta ctgattaatt
    60061 taaactgggc catacggatc gctttaatct gtttatttag cagtgcgcgt tccccttcgt
    60121 tggcggtttt atcacgctgt gctaccagtt cagcgccttt atttgccagt acctcaaatg
    60181 cgtccattat ctttcccccc ttttttcgat tatttaaaag cgcttattga cacgccaaaa
    60241 cgacgactta attagcaaat gagtcgccgc tttgcagtgc cttccatatc ttccggcatt
    60301 tcagactgaa gtttatcagc tacttcatca acaagtggac gaaccgtttc cacgaacttg
    60361 tccgcaatat aatcagcaga acgcatcact ttctcatgga attcctcacg gcgttcctgg
    60421 gcggctgatt taggggctgt cgtgcgctga aaataactgt ctaatgaatg catggtattt
    60481 actactgtgt ttatagtgat tgttcaatta attgcggttg aagccattgt gtgtggccca
    60541 gccgaataag tttgcggacg tttttatgta tccggcggtg cttttctttc ggcatcaggc
    60601 gaatattctt gatgcggtta tcctgtttgt ccaaattaac gtggtgcact tcatataatg
    60661 tctcgtcata agcgccgtat gtgtatacat agcaggcttt ctgataggtg agtgtcactt
    60721 tgaatttacc ttcatgtacc gttacaacca aatgcccttt attggtcatg ctgcctttga
    60781 taacgtcttt tgtttcacgg ctaaacaata aacccgcttc agggtcataa atccatctgc
    60841 gccgcataat atcggcacag cgttctaatc tttcgatatt ggtcatgatg tttacctcaa
    60901 aatgtgatgc gtgattatta tcgttctgtt ctgtacaacc attctttgca ttcgtgctcc
    60961 cagaataatt ggcgagtacc gcgtggggta aatggcaaag ggactccggt tgatggtttt
    61021 tttagccagc gcctcagcgt tctggtcgat acattcgcca ttgctgcgaa ctgttctaat
    61081 gttattgctt tcataaatac ctcgatacga aaaagaagcc gccccacgga ggaaagaaga
    61141 agcaaatttt gaatttccgt gaggcggtgg attccgattc cctgaaccca ttacccatgt
    61201 gatacttaat attataaacc ttattttttc tgttgacatt tttggccata tattgacagc
    61261 ggtttttgtt agcaaaaagt gctatttatt aaatcatcaa agcaataaaa gattatttgc
    61321 acccgcaagg taatggcggg ataatattag cgagggttag ccgccctcga cctcaaagtg
    61381 atctgttaat aactacaacg atatgtctct atttcccgct tctccggcgg gttttttttg
    61441 cctgcaaatc accacatcag aattaattaa attgataccg tttaaaacgc tcccaatcgc
    61501 aatataacgg ccttaacttc tggctatgca tttgctcagg tgtgatcaaa ttgattcgtc
    61561 aggcgtggaa taaggcaata aaaacgggca ttgcgcccgc ttgtgttcac tgcatcgaaa
    61621 ataacttatc cataattctt ttctcctatt ccctgcacat catttcggca cggtcccgat
    61681 cttcctgtgt cggattaaag tccggcgttc tgtatctgtt gcctgaaata taccgtgcat
    61741 gattgcggcg acctgaacgc ggcattatcc agtattcgat gttctcacgc gtggcaatga
    61801 gtaaaccatc ttctacaagc gtattcaggg cgtgccatag ctgcttgtaa gtaacgtaac
    61861 cttcaccgac aagataatcc agtgcgcacg acaatccgct tactgttaac tctttggcgg
    61921 cgaaatttgg gtgttcagaa aggaaatact cctgaccctg tttgctgaat acccaaagga
    61981 tctggcggcg caattcttca acgtcctggc tataagcgtg attgatatgt ctttcagcta
    62041 cgaaagcgcg gccttgtttc ctgattagcc cattactaac catcgtcatg agtgagcggc
    62101 gggcggcgtc aacctggacg cgggtaacat tgccaccata aacgagatct gcggcttgtc
    62161 gtgcggtgat tgattcgttt tttgccagat agtcgaggat ctgccgttgt tgttctgata
    62221 tgcgcatgat gtgtaacccc ctgattttca caataaactt atctcttttc tgcttaatgt
    62281 atgtatctat tatgcgctca atatcgaggc ggggcaatgt tgcgatcagt cctgatcgcc
    62341 accattcagg cgagtgagaa aaatttaatg aaatcgcatc gattttaagc gctctcaatc
    62401 gctctgtggc gacgttaagt tttcaggtaa gcaagttagc gcttatgtgt gttcatcgcg
    62461 ttctatgggc cattcctttc gaatttggcg cggtcatgat agttcgcggt gatcggcggc
    62521 ggggataaag tttcctgagt gatcacttaa gcctgctgtg cgtcgtgtga gtgtgctgtc
    62581 tgcacctggc tatacgctgc tttgctgctg tttcctgtct ggtctgtata cgcgataaaa
    62641 accattggtc ttgcagtgtg gcggtgtcgt tggtttttat tgagcgcgta gcgcgataga
    62701 aggtgcgctc tcagatgttt atgtgtctac ctatggtgag cgctccgtgc cttacgtcgt
    62761 ttcattgagc attggcccca ggagacgagc gaagcgaagc tctccccact gctaagctgg
    62821 ttttgttttt atcctgcatg ctattccgct taagccagct agttaattcg tcaaggaatg
    62881 atttaaagag tcctttaaca ttatggtcac aatccgaaca gctttatcgt gaggatttga
    62941 cctagccgag cggcaatgag cgtagtcctg ctgcacagaa gttaaggtct gctctggagg
    63001 ttatcaggac gtaagcacta gtctcatgat tccgccgaaa agacgaaaaa tcttggcgct
    63061 gatctgtatt tattatttct ctgtatttat tactttgggc cgcaaaaatg cggctagtga
    63121 gtgggcaaat ttgcggctag tgagtgggca aaaatgccca cctatttttt ttcagcgtca
    63181 aaatcatggc aaaatcatga cctttctgga tgcttcttga acagtaatca cgctttttct
    63241 tgcactaacc aattgaataa taaaaggttc tggcaaatag gaccgcattt ttgcggctta
    63301 ttttgcttgc agccagcgcc acgtctgggt ttgagctatc actatcaaac tggttgtaac
    63361 cgcgatgtga ttattagttt attgaggtaa tgatctgatt atctgacatg aaaaaagggg
    63421 catttgcccc cctttgctga cccgctgcct agtgtaccag gtagctgact tttgcttctc
    63481 tttcgtgctg ctgtgccgtc attgaggcct cttctgctgt gccttttgac gggattcgca
    63541 gtctgtaggt ttttccacat ttgcccctca gtgcctggcg catttcctgg ataagtccca
    63601 gctttctcaa cgcttccagg tggcgatcta tcgtgtcaac gctcttacct aacgcttttg
    63661 ccattttggt gcgggaatac caaatctcat atttgtcgtt accgcgatac ctgcacttgg
    63721 ataccatcca cgtatatagc tttaactcat cgcctttcag cttgtgaaac agcccgcttt
    63781 tgaagaggta agccatagcg ttattgacgg ctggaaggtt gcgataatcg ttttgctggt
    63841 cttccgtttc cacctgttcc cgctcttcag gcaactgctc ctctttacta tcaaaacgca
    63901 aatcttcttc cgcttcttcg ttaaaccatg aagtcggctt gtctgtgttg gctttcgcta
    63961 ctggcttggg cattgccagc gggtggcggc attcttcttg ctgcgctgtg atttgttgtc
    64021 tttgcagctc cttgaactcg gcaatagccg ccgctttatt ctgttcctgt tcctgttcct
    64081 gttcctgttc ctgttcctgt tcctgttcct gttcctgctc ctgctcctgc tcctgctcct
    64141 gctcctgcgc tggttctgca ttggctagcg ctggctcttc attggctggc gacaagattt
    64201 gatctatgcg cacgaatcca gttgctggct tgtattccca gcgataccca tcgaagcgga
    64261 tgatgttcat gccatcacga aaggcggcct gaacctgttc cggaagggct tggagtgttt
    64321 gaagtgcttg gagtgttgca attttgtgtt tgaccagtgt ttcgcctggt ttcgccacgt
    64381 aggcggttgc tgattggtac atggtatttt tttcctgtgt tgtttttgtg tccccctagt
    64441 gtgctgtagc ctccagggtg tttttgtgtt gatcctattt tcgatccccg atccaccctg
    64501 ttgcaaacac ggctttaagt cattgatatt tattcattta ttccaacgat actggaataa
    64561 ttggaataat tgatcccatc ggcaaagaca aaaaaagccg gacgaatccg gaaaaagtga
    64621 atagttattt tgaaggtgtc cagtgtttat ccggcctact ccggtggagt cgtcggaaaa
    64681 cgtgattaat cattcattac agttcaagcg gattgatacc atcagccagt atcgaatcaa
    64741 tcgcgtcggc gtagtcctga agcatttgac ggcgcatttt gagataggcc gcttggttgt
    64801 aaactcccct aattcccttc gggacgtggg ctagttggat ctcaatccaa tcagggttat
    64861 acctgccgct ttcgttaagt gcggtgctga acgttgtgcg ccagccgtgg cctgtggcta
    64921 ttgacttgta tccgatacgc tcaagcgcct tgagtatcgc gctggctgat aatggcttat
    64981 ccatgaatcg gcccgggaac acgaaaacac tgtatccgct gcgtgtgaac tggtcctgca
    65041 atagctcgac cgcctgccgt gatagcggga ccacatgcgg gcggcgggcc ttcatgactt
    65101 cagcgggcaa tgtgattgtt gcgtcctcca ggtttatcca ttcccaacgg cacaaacgca
    65161 gttctgcggt ccttaatgcc gttaatataa gcaaacgtat tgccgcctga atctggacgc
    65221 tcccggccct ctcaagggct ttgtagaagg ccggaatttc actaatcggt atccacgcgt
    65281 tgtgaccagg tcggcgggcc ttcagtgccg tatcaagatc tgcaaccgga ttcctcgtta
    65341 ccagttcggt gataacggca aacttgaaga tctcaccgat gcgctggcgt agcttgcggg
    65401 cctgctccgg tgcggtacgt tctttcttct ggagtaatgc cagcacgtca cgggcggtta
    65461 tatcaacgat tgaacgcttg ccaagtaccg gataaacgtc tttctccagt gctgatttga
    65521 cctgctttgt atagctctca ctccagccgc ctaccctggt agccagccag tcctccgcta
    65581 ccgtcttaaa acctgccgcc atgttgagtg catcaatggc tttctccttg cgtgccaaat
    65641 ccattgggtc aattccctgg gctatcagta aacggctctc tgtggcgcgt ttgcgggctt
    65701 ccttgaggct aatcagcgga tataccccaa tagcctgtaa ccgctctttc ttgtctatcc
    65761 agtaccggaa tcgccagtat ttgctgccgt tgggatgcac cagcaaaaac aggccgggcg
    65821 tcagtgaagc ggtgagtttg tattgcttgt cctttggttg tgcggcgtct atggcctttg
    65881 ctgtgagtgg tgttttgctc aaaaccggat acctcgatct aacgactcga taaccccgct
    65941 catgtatccg gcgagttcgg ttgcgttcgg caggataagt aacgggcgtg ttaaggaaat
    66001 caccttacat gacaagggct aacgatgaaa tagaattgtt tgcaggctgt gagaaatgtt
    66061 actcatcact tcttcctgag ggaacgactt atgaaactaa agaccacatt gttcggcaat
    66121 gtatatcagt ttaaggatgt aaaagaggtg ctggctaaag ccaacgaact gcgttcgggg
    66181 gatgtgctgg cgggcgttgc agcggcaagc tcacaggagc gcgtggcggc aaagcaggtg
    66241 ttgtcggaaa tgaccgtagc ggacatccgc aataatccgg tgattgccta tgaagatgac
    66301 tgcgtgacgc ggctgattca ggacgacgtt aacgaaacgg cctacaacca gattaaaaac
    66361 tggagcatca gcgaactgcg tgagtatgtg ctgagcgatg aaaccagcgt ggacgacatt
    66421 gcctttaccc gcaaagggct gacctcggaa gtggtcgcgg cgatagcgaa gatttgctcc
    66481 aacgcggacc tgatctacgg cgcgaagaaa atgccggtaa tcaaaaaggc caataccacc
    66541 atcggtattc cgggcacctt tagcgcccgt ttgcagccga acgatacccg tgacgacgtg
    66601 caaagtatcg ctgcgcaaat ctatgaaggg ctttccttcg gggtgggcga tgcggtgatc
    66661 ggcgttaacc cggtaactga cgacgtggaa aacttaagcc gcgtgctgga taccatttat
    66721 ggcgtgatcg acaaattcaa catcccaact cagggctgcg tactggcgca cgtcaccacc
    66781 cagatcgaag cgattcgtcg cggcgctcct ggcggactga ttttccagag tatctgtggc
    66841 agcgaaaaag ggctgaaaga gtttggcgtg gaactggcga tgctcgacga agcgcgcgca
    66901 gtgggcgcag agttcaatcg tatcgccggg gaaaactgcc tctacttcga aaccggacaa
    66961 ggctctgcgc tatccgctgg cgctaacttc ggcgctgacc aggtgacgat ggaagcacgt
    67021 aactatgggc tggcgcgtca ttacgatccg tttatcgtca acaccgtggt tggctttatt
    67081 gggccggagt atctctacaa cgaccgccag attatccgtg ctggcttaga agatcacttt
    67141 atgggcaagc tgagcggcat ctctatgggc tgtgacggct gctacaccaa ccacgctgac
    67201 tctgaccaga acctcaacga aaacctgatg atcctgctcg ccaccgcagg ctgcaactac
    67261 atcatgggga tgccgctggg tgatgacatc atgctcaact accagaccac cgcattccac
    67321 gataccgcca ctgtgcgtca gttactcaac ctgcgcccgt caccagagtt tgaacgctgg
    67381 ctggaaagca tgggcattat ggcaaacggt cgcctgacca aacgggcggg cgatccgtca
    67441 ctgttcttct gatgacgcgg agataacaca tcatggatca aaaacagatt gaagaaattg
    67501 tacgcagcgt gatggcgtca atgggacaag cggcccccgc gccgtcagaa gcaaagtgcg
    67561 ccaccaccaa ctgtgcggca ccggtgacct cggaaagctg tgcgctggat ttaggttccg
    67621 ctgaagcaaa agcgtggatt ggtgttgaaa acccgcatcg cgcagacgta ttaacagaac
    67681 tgcgccgcag caccgtggcc cgcgtttgta ccggtcgtgc cggtccgcgt ccacgtactc
    67741 aggcgctgct gcgtttcttg gccgatcact ctcgttcgaa agataccgta ctgaaagaag
    67801 tgccggaaga gtgggtgaaa gcgcagggct tgctggaagt acgctctgaa attagcgaca
    67861 aaaatctcta cctgactcgc ccggatatgg gccgccgcct gtgtgcagaa gctgttgaag
    67921 cgctgaaagc acagtgtgtt gctaatccgg acgtacaggt cgttatttct gatggcctgt
    67981 caactgatgc catcaccgtc aactacgaag agatcctgcc gccgctgatg gcgggcctga
    68041 aacaggccgg gctgaaagtc ggcacgccat tctttgttcg ttatggtcgc gtgaagattg
    68101 aagatcagat cggtgagatc ctcggcgcga aagtggtgat cctgctggtg ggggaacgtc
    68161 cggggttagg gcagtcagaa agcctctcct gctacgccgt ttactcgccg cgtatggcga
    68221 ccaccgttga ggccgatcgc acctgtatct ctaacattca ccagggcggc acgccgccgg
    68281 ttgaagccgc agccgtcatt gtggatttgg cgaaacgtat gctggagcag aaagcatccg
    68341 gcatcaacat gacccgataa ggaggcatca tgccagcttt agatttgatt cgaccgtcgg
    68401 taaccgccat gcgggtgatt gcctccgtta acgctgaatt tgcgcgtgaa ctaaaattgc
    68461 cgccgcatat tcgtagcctc gggctgattt ctgccgattc tgacgacgta acctacattg
    68521 cggctgacga agcgaccaaa caagcgatgg tcgaagtggt atatggtcgc tcgctgtatg
    68581 cgggagcggc acacggcccg tcaccgactg ccggtgaagt gctgattatg cttggtgggc
    68641 caaacccggc ggaagtgcgt gccggtctgg atgcgatggt tgcacatatt gaaaatggcg
    68701 cagctttcca gtgggccaat gacgcagaaa acaccgcctt cctggcgcat gtagtttcgc
    68761 gtacaggttc ttatctttca tcaaccgccg ggatcacgct tggcgatccg atggcgtatc
    68821 tggtggcacc gccgctggaa gcgacctacg gcattgatgc cgcgttgaaa tctgccgacg
    68881 tacagctggt gacctacgtc ccgccaccgt ctgaaaccaa ctactcggca gcatttttaa
    68941 ccggtagcca ggccgcgtgt aaagcagcct gtaacgcctt taccgatgca gtgctggaaa
    69001 tcgcgcgtaa tccaatccag cgtgcgtaac ggaggttgcc gatgatcaat gcactgggat
    69061 tgctggaagt ggacggcatg gtcgccgcga tagatgcagc ggatgccatg ctcaaagcag
    69121 ctaacgttcg tctgctcagt cacgaagtgc ttgaccctgg tcgcttaacg ctggtggtgg
    69181 aaggcgatct ggcggcgtgt cgtgcggcgc tggacgcagg ttgtgctgcc gcgatgcgta
    69241 ccgggcgtgt catcagccgt aaggagatcg gtcggccaga cgatgacacc cagtggctgg
    69301 ttactggctt taaccgccag ccgaagcaac ccgtaaagga acccgacgcg ccagttatcg
    69361 tcgcggaatc tgctgacgag ttgttggcgc tgttaacatc agtatgtcag ggaatgacgg
    69421 caggagaagt ggccgcccac tttggctggc cgctggaaaa agccagaaat gcgctcgaac
    69481 agctcttttc tgccgggacg taacgtaaac gcagtagtcg ttatcgtctc aagccccatt
    69541 aacctgtcgg aggtgccggg tgtcctacaa cacccggcat taacatcatg aaaaagaccc
    69601 gtacagccaa tttgcaccat ctttatcatg aacccttacc cgaaaacctg aagctcacgc
    69661 cgaaggtcga agtggataat gttcatcaac gacagacaac ggatgtctat gaacatgctt
    69721 tgacgattac cgcctggcag cagatttacg atcagctgca tccgggcaag tttcatggtg
    69781 aatttacgga aattctactc gatgatattc aggtttttcg tgaatacacc ggtctggcgc
    69841 tgcgtcagtc gtgcctggtc tggccgaact cgttctggtt tggcattccg gcgacgcgcg
    69901 gtgagcaggg atttatcggt tcgcaatgtc tgggaagcgc agaaatagca acgcgccccg
    69961 gtggaaccga gtttgaatta agtacgccgg acgattatac gatcctgggc gtagtgctgt
    70021 ctgaagatgt catcacctgt caggctaact ttttgcacaa tccggatcgg gtattacata
    70081 tgctgcgtag ccagtcggcg ctggaagtga aagagcagca taaagccgcg ctgtggggct
    70141 ttgtccaaca ggcgctggcg acgttttgcg agaacccgga aaatctccat cagcctgcag
    70201 tgcgaaaagt gctgggggat aatttgctaa tggcgatggg ggcgatgctg gaagaagcgc
    70261 aaccaatggt gacggcggaa agcatcagtc atcagagtta ccgtcggttg ctttcccgcg
    70321 cccgtgaata tgtgctggaa aacatgtccg aaccggtgac ggtgctggat ttgtgtaatc
    70381 aactgcatgt cagtcgccgc acgctacaaa acgcgtttca cgctatttta ggcattggcc
    70441 caaacgcgtg gctgaaacgc attcgcctga acgccgtacg ccgcgaactg ataagcccgt
    70501 ggtcgcaaag cacaacggta aaagacgccg ccatgcagtg gggattctgg catctggggc
    70561 aatttgccac ggattaccag cagctgtttg ccgagaagcc gtcgttgacg ttgcatcagc
    70621 ggatgcggga atggggggga gggagttaca cccaatccct gaccttaata aactcactta
    70681 acgccgcttc tgggctgcca tcttttggct gataatcata ctcccagcgt accagtggcg
    70741 gcattgacat caggatcgac tcggtgcgcc cgccagtttg caggccaaac agcgtgccgc
    70801 gatcccagac cagattgaac tcgacataac gaccgcgacg gtagagctga aaattgcgct
    70861 cgcgctcgcc gtaggccatc gctttacgtc gctctacaat tggtaaataa gcgtcggtgt
    70921 agcctttgcc taccgcctgc ataaaggcaa aacagtggtc gaaatctggc gtgttcagat
    70981 catcaaagaa cagcccgcca atgccgcgct gttcgttgcg atgtttgagg tagaagtaat
    71041 cgtcgcacca ctttttgtaa cggggataaa cgtcttcacc aaatggctgg cacaggtcac
    71101 gggcggtgcg gtgccagtga atggcgtctt cttcaaaacc atagaaaggg gttaaatcga
    71161 agccgccgcc aaaccaccaa acgggctctg cacccggttt ttcggcaata aaaaaacgca
    71221 cattcgcgtg gctggtggga acatacgggt tatgcggatg cactaccagt gaaacgccca
    71281 tcgcctcgaa actgcgcccg gcaagttccg ggcgatgagc ggtggcggaa gcaggcatcg
    71341 cctcaccgtg gacgtgagaa aagttgacgc ccgcctgttc gaaaacacca ccattacgca
    71401 acacccgact gcgcccgccg ccgccagctt cgcgctgcca actatcttcg acaaattctg
    71461 cgccatcgac ggcggtcagc tgctgacaaa tcgtatcctg aaggttgagc agaaactgtt
    71521 taacctggtg tgcgtcgggt ttcataactt atcgcttttt cgaatgtgct ttctggttgt
    71581 cgaaccagtg gaaataactg atcacgcctt cagcaatcgc tgtggcgatt ttctgacgaa
    71641 acgccgccgt gcctaacagc cgctcttctt ccgggttggt gataaacgag gtttccacca
    71701 gcaccgaagg aaccgacggc gatttcaaca ccacaaacgc ggcttgttcg gtgttgcggc
    71761 tgtgcagttt atgcaccggc ttaatctttt tcagaatatg cgagccaagc gtcaggctgt
    71821 ttttaatagt atcggtttgc accagatcaa acagtacttg ctgcaatagg tgatccttgt
    71881 cagtcgcctt tttaccggca acttcatcgg cgcggttttc gcgttcagac aggtatttcg
    71941 ccattgcgct acttgcccca cggttagaga gggcaaatac cgaagcaccg gcagctttcg
    72001 ggttggtaaa gccatcggca tgaattgaca taaacagatc tgcgccatgt ttatgggcga
    72061 tttcaacgcg atcgtaaagt gggataaacg tatcgccaga acgcgttaaa cgcgcatcaa
    72121 tcccatgatt acgcaaaatg gaacggacgt ttttagcaat cgccagcacc acatgttttt
    72181 ctttcgaacc gttgcgtccg atcgctccgg tatcaattcc gccgtggcct ggatcgagaa
    72241 caacgacacg tttgccgcca gatttttttg ctttcggctt gctgtgtccg ttgctggttt
    72301 ttaaaggttc gtctttggca atggcttgcg acattcctga caacgtcagg gcagctaaac
    72361 cggctttcag cacctggcgg cgcgaagtga gtgtttttag tggtttaaaa gtgctcatac
    72421 ggcctgagtt gtaataataa agttccagat gttatatcgt atcgcgtaat ccgttacgac
    72481 ggtttgctgt tgagaattgt tttacttttc atttcaatag ttgacaactc cccattatgc
    72541 cactttttca accgattttc gtcagatatt ggctaattcg accgttcacg ccataatcag
    72601 ccttttaacc cgcaaaaagg taacgaatac catggagata cgcgtatttc gccaggaaga
    72661 tttcgaagag gtcatcaccc tttgggagcg ttgcgacttg ctgcgtccgt ggaacgatcc
    72721 ggaaatggac atcgagcgta agatgaacca tgacgtcagt ttgtttttgg tcgctgaggt
    72781 aaacggtgaa gtggtcggaa cggtgatggg cggttatgac gggcatcgcg ggtctgctta
    72841 ttatcttggc gtgcatccag agtttcgtgg gcgtggaatt gccaatgcgt tgcttaaccg
    72901 gctggagaaa aagctgattg ctcgcggctg cccgaaaatt cagatcaacg tgccggaaga
    72961 taacgacatg gtgctcggaa tgtatgaacg cctgggatat gaacacgccg acgtgctgag
    73021 tctgggtaag cgtttgattg aagatgaaga gtactgagtt ccatcctgtc cattatgatg
    73081 cgcacggacg cctgcgttta cccttgctct tctggcttgt gctattgctt caggcgcgaa
    73141 cctgggtgct gtttgtcatt gccggtgcgt cgcgcgagca gggtactgcg ctgctaaatc
    73201 tgttttatcc cgatcacgat aatttctggc tggggctgat tcccggtatt ccggcggtgc
    73261 tggcgttttt gttgagcggt cggcgggctt cgtttcctcg cacctggcat gtgctctatt
    73321 ttctgttgct gttggcgcag gtggttttac tttgctggca accgtggctg tggctgaacg
    73381 gtgaatccgt tagcggtatc ggtctggcgc tggtagtggc ggatattgtg gcgttaatct
    73441 ggctgctgac caatcgacgt ttacgcgctt gttttaatga agaaaaagaa taacggcact
    73501 ttttggtgaa tttgcactcc aagcaacgtt attgaataac caaaggaagt gacatgaaat
    73561 cgctgcgttt aatgttatgc gctatgccgt tgatgctgac cggctgttcc acgatgtcgt
    73621 cagttaactg gtctgccgct aacccgtgga actggtttgg atcgtccacc aaagtgagcg
    73681 agcagggcgt gggtgaatta acggcgtcca caccactgca agaacaagcc attgctgatg
    73741 cgcttgatgg cgattatcgc ctgcgcagcg gaatgaaaac cgcgaacggc aacgtggtgc
    73801 gcttttttga agtgatgaaa ggcgacaacg tggcgatggt gattaacggc gatcaaggca
    73861 cgatcagtcg cattgacgtg ctggatagcg atattcctgc tgacaccggt gttaaaatcg
    73921 gtacaccgtt tagcgacctt tacagcaaag catttggcaa ttgccaaaaa gctgatggtg
    73981 atgataatcg tgccgtcgaa tgtaaagccg aaggcagtca acatattagc taccagttca
    74041 gcggggaatg gcgtggtcct gaagggttaa tgccttcgga cgataccctg aaaaactgga
    74101 aagtcagtaa aattatctgg cggcgttaat ttgcgtctga acaaaccgcc gtcgcgaaaa
    74161 aacacgtaaa atagcgctca acaatgccac ggattgcgtg gcattcttat tttcaggagg
    74221 aacaatgtct caggttcaga gtggtatttt gccagaacat tgccgcgcgg cgatttggat
    74281 cgaagccaac gtgaaagggg aagttgacgc cctgcgtgcg gccagtaaaa catttgccga
    74341 caaactggca acttttgaag cgaaattccc ggacgcgcat cttggtgcgg tggttgcctt
    74401 tggtaacaac acctggcgcg ctctgagcgg cggcgttggg gcagaagagc tgaaagattt
    74461 tccgggctac ggtaaaggcc ttgcgccgac gacccagttc gatgtgttga tccacattct
    74521 ttctctgcgt cacgacgtaa acttctctgt cgctcaggcg gcgatggaag cctttggtga
    74581 ctgcattgaa gtgaaagaag agatccacgg cttccgttgg gtggaagagc gtgacctgag
    74641 cggctttgtt gacggtacag aaaacccggc gggtgaagag acgcgccgcg aagtggcggt
    74701 tatcaaagac ggcgtggatg ccggcggcag ctatgtgttt gtgcagcgtt gggagcacaa
    74761 cctgaagcag ctcaaccgga tgagcgttca cgatcaggag atgatgatcg ggcgcaccaa
    74821 agaggccaac gaagagattg acggcgacga acgtccggaa acctctcacc tcacccgcgt
    74881 tgatctgaaa gaagatggca aagggctgaa gattgttcgc cagagcctgc cgtacggcac
    74941 cgccagtggc actcacggtc tgtacttctg cgcctactgc gcgcgtctgc ataacattga
    75001 gcagcaactg ctgagcatgt ttggcgatac cgatggcaaa cgtgatgcga tgttgcgttt
    75061 caccaaaccg gtaaccggcg gctattattt cgcaccgtcg ctggacaagt tgatggtgct
    75121 gtaagcgttt ctgattcatt gattaaaggc caggcagtaa cattactgac tggccttttt
    75181 attactgctt taacgccgca tatacttgat caactacctt ctgtcagaac agggtatttt
    75241 gcggcgctga cgctacaact aacagccagc agagaaagat agagcattgt ccgtttcata
    75301 atatcagtcc agattgacgt tacggcagcc aaagagcgtg gtgaaaataa acccgcaaac
    75361 ccatgccacc agaatcccgc cagcataaac cgccattgcc ggaaggatgc cttgtgcgga
    75421 agtcatcagc ggcagcgcca ccagaccaga cggcccaaag gcgctgttta agcccatcgg
    75481 cagaccccac caggctatca atccgataaa caaaccgccc gccgcgccgc ctaaacaggc
    75541 ggtaacaaac ggcttcatgc ggggcagggt gacaccgtaa atcagcggtt caccaacgcc
    75601 cagcaggccg ggaataatcg ccccgcgtac ctgactgcgt agcgcactgt gcggttgcgc
    75661 ccgccagtag agtgccagcg ccgcgcccac ctggcccgcg cctgccattg aaaggatggg
    75721 aaataagctg ttgaatccct ggctgtccat taacgcgagg taaacaggaa taaagccctg
    75781 atgcacgcca aataccacgg cgatcaggaa cagcccggct aaaaccgcac aaccgaacgg
    75841 attactgttc aggtgcataa acagccatga cataccttcg aataaccagc cacccagcgg
    75901 cataatgatc aggtacgcaa gcgtagcggt gatcagcagg gtgattaacg aggtcagcag
    75961 catgtcgaga tcgtccggca taaagcggcg caccatgcct tcaatgcggg cgcaggccct
    76021 cgcggcaatc agcacgccga taatattgcc gcgcggatcg atgggcagac caaagaaatc
    76081 gtgaaaaccg gcgtagtaac cggtggttgc agcggggtta taaccgagca aaaagagtgc
    76141 ggcaataatt gcgccattga cgcccgtgcc gccaaatgcc tgggcggcgt tatagcccac
    76201 cagaatcact aggaaggtga acaaaccttt gctgaacacc ttcataaaat tcagcgcgtc
    76261 gggtagcgtg ccctgagcat ctgccggaac gtgcatcacc gtggcaatta acgtcgctat
    76321 ccccagcagc agaccggcgg caataaaacc ggggatcagc ggcgtgaaga tggtggcgaa
    76381 tttggcgaga aattgttgca cgccggaggt ttgtttggct tttaactgac gtttgttctg
    76441 ggcggcgatt tctgcggcat cctgtaccgg tgcctcgccc agcagctcgc tcatcgcttt
    76501 tgcggcacga tgcgctttac caggcccaaa aacgacctgc acctggtcgc tggtcaaaat
    76561 gacgcccttc acgccttcca gcgttttgat attgggatca accagtgaac tgtcatgtac
    76621 acccagacgc aggcgcgtca tacagttacc acaactggcg atatttcccg gtccgccgac
    76681 acgggtaaga atggtgttca gaagttcact gctgatctct ttggccatgg gttattcctt
    76741 gtctaaaacc tgacgaataa agccgccgtg ttgatccagg cgttttttag cttctgcggc
    76801 atcgagattt ttcagcacca tcacaatggc cgttttacag ttgcgctcgc aggcaattaa
    76861 cgccgcttcc gcttgttcgg cattacatcc ggtggcgttt ttaacgatat tgacctggcg
    76921 cacatgcagt ttttcgttgg tggcgaccac atcgaccatc aggttgccga acactttgcc
    76981 ggatttaatc atcagcccgg tggaaagcat attgagcacc agtttctgcg ctgtgcctgc
    77041 tttcatccgc gaagaaccgg taacaacttc ggcacctaca atcggtgtaa tggcaaactc
    77101 agcggtggtt gaaacggcgc tccccggatt acaggaaatt cccactgtgc ggcagccgag
    77161 ctggcgtgcg tattccagtc cggcaatcac atacggcgtg cgaccgctgg cagcaatgcc
    77221 aaccaccaca tcctgtgccg ttaaattaat atttttcaga tcgttaacac caccttcccg
    77281 gctatcttca gcgccttcca ccgcgtgctg aatggcatat tcgcctccag caatcaaacc
    77341 aaccaccaga cccggtttca cgccgtaggt gggcggacat tcgctggcat ccagaatccc
    77401 cagacgaccg gatgttcccg caccgagata aatcagacga ccgccgccgc tgacctgggc
    77461 gtggataaca tcgatcgccg cggcgatatc cggcaggacg cgatcaacgg caagcggtac
    77521 ggttttatct tcatcgttga taatccggca catttccagc gtcgatacgc ggtcaatttc
    77581 agccgaggcg gtgttcgagc cttcagtaat catcttttct agttgcatcg gtggtgacct
    77641 tactatttac gatcaaataa ttgcagatgt atggaatcat atatcccgaa ttgacagact
    77701 taaaggaatc attgagtcta cactcgcaga gtgtgaagag cgtcataaga acttaagcgg
    77761 aatttttgct gccagcccag gagagtggaa gatgttgtac ctgacgaaaa tacgcaatgc
    77821 ggaaagtgaa tttaccggga acgagcaaaa aattgctgac tttctgcggg ccagagtgag
    77881 cgagctgaaa tccgtctctt cacggcagat ggcgaaacag ctcggtatca gccagtcgag
    77941 cattgtgaaa tttgcccaga agctcggcgc gcaaggattt actgaattgc gtatggcgtt
    78001 aattggcgaa tacagtgcca gccgggagaa aacgaatgcc acggcgctgc acctgcacag
    78061 ttcgattacc agtgatgatt cgctggaagt gattgcccgc aaactgaatc gcgaaaaaga
    78121 gctggcgctg gagcaaacct gtgcgttgtt cgactacgcg cggctgcaaa aaatcattga
    78181 ggtcatcagt aaagcaccgt ttatccagat aacgggcctg ggcggatcgg cgctggtggg
    78241 gcgcgatctg tcgttcaaac tgatgaaaat tggctatcgc gtggcctgcg aagccgacac
    78301 gcacgtacaa gccaccgttt cccaggcgtt gaaaaaaggc gatgtacaaa tcgctatctc
    78361 ttacagcggc agtaaaaaag agattgtctt gtgcgcagaa gcggcacgaa agcagggggc
    78421 aacggtgatt gccattactt ctctggctga ctctccgctg cggcggctgg cgcatttcac
    78481 gctcgatacc gtttccggtg aaaccgaatg gcgtagctcg tcgatgtcta cccgcacggc
    78541 gcaaaattct gttaccgatt tactgtttgt tggtcttgtg caacatcaat gatgtggcgt
    78601 cattgaaaat gattcagcgc agcagtgagc taacacaacg cctgaaatga tgcataaagc
    78661 agcgactgga ttgagatttt cctgaattag tgagctgatc cgcagcaata ttttgtttat
    78721 cctgtatttt cagagggaat ggagtgtaac gctctgtatt aacaaggaga gcattaaaat
    78781 gggtaaactc acgggcaaga cagcactgat tacgggcgca ttgcagggaa ttggcgaagg
    78841 aattgccaga acttttgcac gtcatggcgc gaacctaatc ttgctggata tctcccctga
    78901 gatcgaaaag ctagcggacg aactgtgtgg tcgtggtcat cgctgtacgg cggttgtcgc
    78961 cgatgtgcgt gacccggcgt cggtagccgc agctatcaaa cgcgcgaagg aaaaagaagg
    79021 gcgcattgat atcctagtga ataacgcagg cgtttgtcgt ctgggcagtt tcctcgatat
    79081 gagcgatgaa gatcgcgatt tccatattga tatcaatatt aaaggcgtat ggaacgtcac
    79141 gaaggcggtg ctgccggaga tgattgcgcg caaagatggt cgcattgtga tgatgtcttc
    79201 agtcactggt gatatggtgg ccgatcctgg cgaaacggcg tatgccttaa cgaaagcggc
    79261 gattgttggc ctgactaaat cgctggcggt ggagtacgcg caatccggta ttcgcgttaa
    79321 cgccatctgc ccgggatacg tccgcacgcc aatggcggaa agcattgccc gccagtcgaa
    79381 cccggaagat ccagaatcgg tgctgactga aatggcaaaa gcaatcccgc tgtgtcgcct
    79441 cgccgatccg ctggaagtcg gcgaactggc ggccttcctc gcatcggatg aatccagcta
    79501 tttaaccggt acacagaatg tgattgatgg cggcagcaca ctgccggaga cggttagcgt
    79561 cggtatctga ttcacctctg tttcctccct gcatgtgtgg ggaggatttc gtcttgaact
    79621 aagttcacca ggctatttca tttgtcattt tggccccggg cagtgctcga aatcctcacg
    79681 tactatgtgt acgctccggt ttctccgcgc tgtccgtgtc caaactgcct gcaacaatta
    79741 cgcctgttga accaagttct tattcccttt ttaacttcca aatcaccaaa cggtatataa
    79801 aaccgttact cctttcacgt ccgttataaa tatgatggct attagaaagt cattaaattt
    79861 ataagggtgc gcaatggccg ttaacttact gaaaaagaac tcactcgcgc tggtcgcttc
    79921 tctgctgctg gcgggccatg tacaggcaac ggaactgctg aacagttctt atgacgtctc
    79981 ccgcgagctg tttgccgccc tgaatccgcc gtttgaacag caatgggcaa aagataacgg
    80041 cggcgacaaa ctgacgataa aacaatctca tgccgggtca tcaaaacagg cgctggcgat
    80101 tttgcagggc ttaaaagcag acgttgtcac ttataaccag gtgaccgacg tacaaatcct
    80161 gcacgacaaa ggcaagctga tcccggctga ctggcagtcg cgcctgccga ataatagctc
    80221 gccgttctac tccaccatgg gcttcctggt gcgtaagggc aacccgaaga atatccacga
    80281 ctggaacgac ctggtgcgct ccgacgtgaa gctgattttc ccgaacccga aaacgtcggg
    80341 taacgcgcgt tatacctatc tggcggcatg gggcgcagcg gataaagctg acggtggcga
    80401 tgaagccaaa accgaacagt ttatgaccca gttcctgaaa aacgttgaag tgttcgatac
    80461 cggcggtcgt ggcgcaacca ccacttttgc cgagcgtggc ttgggcgatg tgctgattag
    80521 ctttgaatcg gaagtgaaca acatccgtaa acagtatgaa gcacagggct ttgaagtggt
    80581 gatcccgaaa accaatattc tggcggaatt cccggtggcg tgggtcgata aaaatgtgca
    80641 ggccaacggt acggaaaaag ccgcaaaagc ctacctgaac tggctctaca gcccacaggc
    80701 gcagaccatc atcaccgact attactaccg tgtgaataac ccggaagtca tggacaaact
    80761 gaaagataaa ttcccgcaga ccgaactgtt ccgcgtggaa gacaaatttg gctcatggcc
    80821 ggaagtgatg aaaacccact tcaccagcgg cggcgagtta gacaagctgt tagcggcggg
    80881 gcgtaagtaa tgtttgcagt ttcttcaaga cgcgtgctgc cgggctttac tttaagcctt
    80941 ggcaccagtc tgctgtttgt gtgcctgata ttgctgctgc cgctctctgc gctggtgatg
    81001 cagctttctg agatgagctg ggcgcagtac tgggaggtga tcaccaaccc gcaggtggtc
    81061 gcggcctaca aagtgacgct gctgtcggcg tttgtggcat cgatttttaa cggcgttttc
    81121 ggtctgctga tggcgtggat tttaacacgt tatcgctttc cgggccgcac gctgcttgat
    81181 gcgctgatgg atttaccctt tgcgctgcca acggcagtcg ccggtttaac gctggcctcg
    81241 ctcttttccg taaacggttt ttacggtgaa tggctggcga agtttgatat caaagtcacc
    81301 tatacctggc ttggtattgc ggtggcgatg gcctttacca gcattccgtt tgtggtgcgt
    81361 accgtgcagc cggtgctgga agagttaggc ccggaatatg aagaagcggc ggaaacgctt
    81421 ggtgcaacgc gctggcagag tttctgcaaa gtggtgctgc cggagctttc tccggcgctg
    81481 gtggcgggcg tggcgctgtc gtttacccgt agtcttggtg aatttggcgc ggtgattttt
    81541 atcgccggaa atatcgcgtg gaagacggaa gtgacgtcgc tgatgatttt tgtgcgctta
    81601 caggagtttg attacccggc agcgagcgcg attgcttcgg tgatcctcgc ggcatctctg
    81661 ctgctgctgt tctcaattaa cactctgcaa agtcgctttg gtcggcgtgt ggtaggtcat
    81721 taatggcgga agttacccaa ttgaagcgtt atgacgcgcg cccgattaac tggggcaaat
    81781 ggtttctgat tggcatcggg atgctggttt cggcgttcat cctgctggtg ccgatgattt
    81841 acatcttcgt gcaggcattc agcaaggggc tgatgccggt tttacagaat ctggccgatc
    81901 cggacatgct gcacgccatc tggctgacgg tgatgatcgc gctgattgcc gtaccggtaa
    81961 acctggtgtt cggcattctg ctggcctggc tggtgacgcg ctttaacttc ccgggacgcc
    82021 agttactgct gacgctactg gacattccgt ttgccgtatc gccggtggtt gccggtctgg
    82081 tgtatctgct gttctacggc tccaacggcc cgctcggcgg ctggctggat gaacataatc
    82141 tgcaaattat gttctcctgg cccggaatgg tgctggtcac catctttgtg acgtgtccgt
    82201 ttgtggtgcg cgagctggtg ccggtgatgt taagccaggg cagccaggaa gacgaagcgg
    82261 cgattttgct tggcgcgtcc ggctggcaga tgttccgtcg cgtcacattg ccgaatattc
    82321 gctgggcgct gctttacggc gtggtgctga ccaacgcccg cgcgattggc gagtttggtg
    82381 ctgtgtcggt ggtttccggc tcgattcgcg gcgaaacact ttcgctgccg ttacagattg
    82441 aattgttgga gcaggactac aacaccgtcg gctcctttac cgccgccgcg ctgttgacgc
    82501 tgatggcgat tatcaccctg tttttaaaga gtatgttgca gtggcgcctg gagaatcagg
    82561 aaaaacgcgc gcagcaggag gagcatcatg agcattgaga ttgccaatat caagaagtct
    82621 tttggtcgca cccaggtgct gaacgatatc tcactggata ttccttctgg tcagatggtc
    82681 gcgttgctgg ggccgtccgg ttccgggaaa accacgctgc tgcggattat cgccgggctg
    82741 gagcatcaaa ccagcgggca tattcgcttc cacggcaccg acgtgagtcg cctgcacgcg
    82801 cgcgatcgta aagtcggttt cgtgttccag cattacgcgc tgttccgcca tatgacggta
    82861 ttcgacaata tcacttttgg cctgacggtg ctgccgcgtc gcgagcgccc gaatgccgca
    82921 gccatcaaag cgaaagtgac aaaattgctg gaaatggtcc agcttgcgca tctggcggat
    82981 cgttatccgg cgcagctttc cggcggccag aaacagcgtg tggcgctggc gcgcgcgctg
    83041 gctgtagaac cgcaaattct gctgcttgat gaaccgtttg gcgcgctgga tgcgcaggtg
    83101 cgtaaagagc tgcgtcgctg gctgcgtcaa ctgcatgaag agctgaaatt caccagcgtg
    83161 tttgtgaccc acgaccagga agaggcgacc gaagtagccg atcgtgtagt ggtgatgagt
    83221 caggggaata ttgaacaggc tgatgcgccg aatcaggtat ggcgcgaacc agcgacccgt
    83281 tttgtactcg aatttatggg cgaagtgaac cgcctgcagg gaaccattcg cggcgggcag
    83341 ttccatgttg gcgcgcatcg ctggccgctg ggctacacac ctgcgtatca gggtccggtg
    83401 gatctcttcc tgcgcccgtg ggaagtggat atcagccgcc gtaccagtct cgattcgccg
    83461 ctgccggtac aggtactgga agccagcccg aaaggtcact acacccaatt agtggtgcag
    83521 ccgctggggt ggtacaacga accgctgacg gtcgtgatgc atggcgacga tgccccgcag
    83581 cgtggcgagc gtttattcgt tggtctgcaa catgcgcggc tgtataacgg cgacgagcgt
    83641 attgaaaccc gcgatgagga acttgctctc gcacaaagcg cctgataggt tgagtgaatg
    83701 ttaaacgccc ggaggcgctt cccgcgatcc gggcttttta atggcaaggt ttgtaacctg
    83761 taggcctgat aagacgcgca agcgtcgcat caggcaacac cacgtatgga tagagatcgt
    83821 gagtacatta gaacaaacaa taggcaatac gcctctggtg aagttgcagc gaatggggcc
    83881 ggataacggc agtgaagtgt ggttaaaact ggaaggcaat aacccggcag gttcggtgaa
    83941 agatcgtgcg gcactttcga tgatcgtcga ggcggaaaag cgcggggaaa ttaaaccggg
    84001 tgatgtctta atcgaagcca ccagtggtaa caccggcatt gcgctggcaa tgattgccgc
    84061 gctgaaaggc tatcgcatga aattgctgat gcccgacaac atgagccagg aacgccgtgc
    84121 ggcgatgcat gcttatggtg cggaactgat tcttgtcacc aaagagcagg gcatggaagg
    84181 tgcgcgcgat ctggcgctgg agatggcgaa tcgtggcgaa ggaaagctgc tcgatcagtt
    84241 caataatccc gataaccctt atgcgcatta caccaccacc gggccggaaa tctggcagca
    84301 aaccggcggg cgcatcactc attttgtctc cagcatgggg acgaccggca ctatcaccgg
    84361 cgtttcacgc tttatgcgcg aacaatccaa accggtgacc attgttggcc tgcaaccgga
    84421 agaaggcagc agtattccgg gcattcgccg ctggcctgcg gaatatctgc cggggatttt
    84481 caacgctgcg ctggtggatg aggtgctgga tattcatcag cgcgatgcgg aaaacaccat
    84541 gcgcgaactg gcggtgcggg aaggaatatt ctgtggcgtc agctcaggcg gcgcggttgc
    84601 tggagcactg cgggtggcaa aagctaaccc tggtgcggtg gtggtagcga tcatctgcga
    84661 tcgtggcgat cgctacctct ccaccggagt gtttggggaa gagcatttta gtcagggggc
    84721 agggatttaa ggattaatag cattggagac tgatgacaaa cgcaaaactg cctgatgcgc
    84781 tccgcttatc aggcctacaa tgtttctgca atatattgaa ttagcaatat ttttatgccg
    84841 gatcaggcgt ttacgccgca tccggcataa acaaagtgca ctttgtcaat aatctgattt
    84901 gtcggcagtt gttgctgccg acaaatgtag tttttaattt tcgtgtggaa aagaagtaag
    84961 gtcatcaatc attttttgaa gtattggtga gtcctgaccg tcaccccatt gaagaatttt
    85021 tgcggtaagc tgatgacgcg ctagtgcatt gagtcccatc tttttaagtt tatgggagat
    85081 tttacccatg actttgatct gaccgaatgc agtcgcgtag gtaatcatat tgctctggat
    85141 agagctcatt tcatcggcta gtaattgcgc acgcacatcc tcttcaagaa tactgtccat
    85201 agcgatgtat ttcatacccc acatagtttc aattaacatt tgagggaaat cagcacagtc
    85261 cagaacggag gctattttca agttcgttta gagcatcagc accttcatcg ctaccaaaag
    85321 gagattcttc atcccagcaa cacatataaa gtggatcagt aaaatggcac tggaagtttg
    85381 ggtgtgacgt tttaagatgt aacccaatct cttcatcatc aaaatagtag agatccatga
    85441 agttaaagtc tggagtttct tgatatcctt ttttcatttt tgcggcaatc aatttactgg
    85501 cttctttcag acactgttct tcattatcga attcttttgt atggaattta ccaatactac
    85561 ctaccttgcc atagttgaca gctaaactat ccccttcgta atctatccac caaaatttat
    85621 tcgatttttc atcgtgataa ataaagcatt ttttcatgtt acctcctgta aatgccttac
    85681 caaagcggat taatttcctt ccgcaggcac cgttttatcc gtctgcgcaa ttaacgcatg
    85741 taaaaaaggc tgtgcgttct catcgctctt accggggatc ttcacgatat acggcttccc
    85801 ggtaatcgat gacgacgaag ctaccttatc aataaactgc tcggcagtgt caatgcggtt
    85861 acgggtattg ccgagcttca gacgcaggtg ggaaaccgct tcatcgcagg tatgttcatc
    85921 accgttgcgc acaaatatca aatccttttt ctgtgctaat ccctccagca tggcgttgat
    85981 gcgggcttct tcatgaacag ttaacttcgc gtaagcgggg agtgtcatta gcagcgtaat
    86041 ggccagacaa atgattttct tcactgaatt accttatctt tgttttgtaa tagccgattt
    86101 gactgccaga atcaggtaaa gtttcggttg gaatacgatg aggatcatcg gcaaggagaa
    86161 aaaattaaat gagtagtttg ttgttgttta acgataagag tagggcgctg caggcggata
    86221 tcgtcgccgt gcagtcgcag gtagtgtacg gaagcgtagg caacagcatt gcggtgcctg
    86281 ctataaaaca gaacggcttg aatgtctttg ccgtgccgac ggtattgctg agcaatacgc
    86341 cgcattatga cactttctac ggtggtgcga ttccggacga atggtttagc ggctatttgc
    86401 gtgcgcttca ggagcgtgat gcgctgcgcc aacttcgtgc tgtaaccacg ggctatatgg
    86461 gaacggcatc gcaaatcaaa atccttgccg agtggctgac tgcgctacgc aaagaccatc
    86521 ctgacctatt gatcatggtc gatccggtga ttggcgatat tgatagcgga atttatgtca
    86581 aacctgacct tcccgaggcg tatcgacaat atttactgcc gctggcgcag ggaattaccc
    86641 ccaatatctt tgagctggaa atcctgaccg gtaaaaattg ccgcgatctc gacagtgcca
    86701 ttgctgccgc aaaaagtctg ctttcagaca cattaaaatg ggtggtgatt accagcgcct
    86761 ccggtaatga agaaaatcag gagatgcagg tggtggtggt cagtgccgac agcgtgaatg
    86821 tcatttccca ttcacgggta aaaaccgacc tgaaagggac tggcgacctg ttttgtgctc
    86881 agcttatcag tggtttgctg aaagggaagg cgttaaacga tgcggtgcac cgtgcggggt
    86941 tgcgcgtact ggaagtgatg cgctacaccc agcagcatga gagcgatgaa ttgattttgc
    87001 cgccgctggc ggaagcataa aaaatggcgc cgatgggcgc catttttcac tgcggcaaga
    87061 attacttctt gatgcggata accggggttt cacccacggt tacgctaccg gacagtttga
    87121 tcagttcttt gatttcgtcc atgttggaga taacaaccgg agtcagggta gacttggctt
    87181 tctcttccag cagcggcaga tcaaattcaa tgacagtatc gccaactttc acgcgctgac
    87241 cttcttcagc aatacgcttg aagccttcgc ctttcagttc aacggtgtcg ataccgaagt
    87301 ggacgaacag ttcaacgccg ctatcagatt cgatagagaa cgcgtgattg gtttcaaaga
    87361 ttttaccaat ggtaccgtct acaggcgcga ccattttgtt acccgttggt ttgatagcaa
    87421 taccatcacc aacgattttt tccgcaaaaa cgacatccgg cacgtcttcg atattgacga
    87481 tctcgccaga gagcggagca atgatctcaa tagttccggt atcctttttg tcgtcggaaa
    87541 ccagagattt cagtttatcg aacaaaccca tgatcttctc ctaagcagta aattgggccg
    87601 catctcgtgg attagcagat tgttttttct tcaatgaact tgttaaccag cgtcattaac
    87661 tcgtccgttg tcggttgagc aagagcctgc tctgctaaca ccttcgcatc ttcgaagttc
    87721 gtgttacgga taatcttctt aatgcgcggg atagaaatgg cgctcataga gaattcgtcc
    87781 agacccatcc ccagcaacag aagtgtagca cgttcatcgc cagcaagctc accacacatg
    87841 ccagtccatt tgccttcagc atgagaagca tcaataactt gcttgatcaa gttcagcacg
    87901 gacggtgaca ttggctggta aaggtgtgaa atcatatcat taccacggtc aactgccaga
    87961 gtgtactgcg ttaaatcatt ggtgccgata ctaaagaaat caacttcttt ggctaaatga
    88021 cgtgcaattg ttgcggcagc cggtgtttcc accattacgc cgatttcaat tgactcgtca
    88081 aacgctttac cttcgtcgcg cagttcctgt ttgtagattt cgatctcttt gcgcagtgca
    88141 cgcacttctt caacagagat gatcatcggg aacataatgc gcaatttacc gaaagccgag
    88201 gcacgcagga tagcgcggag ctgatcgcgc aggatctctt tacgatccat cgcgatacgg
    88261 atagcgcgcc agccgaggaa cgggttctct tctttcggga agttcatgta tggcagctct
    88321 ttgtcgccgc cgatgtccat ggtacgaacg ataaccgcct gcgagccaca cgcttcagcc
    88381 actgctttgt aagcagcaaa ctgttcttct tcagtgggca gcgcgtcgcg gtccatgaac
    88441 aggaactcag tacgatacag accaacgcct tcagcgccgt tacgctctgc accttcaacg
    88501 tcacgaaccg taccaatgtt agcgcatact tctacctggt gaccgtccag cgtaatagct
    88561 ggcagatctt tcagtttagc aagctctgct ttttcagaag ccacttgctc ctgaacagcg
    88621 cgcattttat caataacttc gttggttgga ttgacgtaaa cctgattatt tacggcatcc
    88681 agaatcagat agtcgtcatt tttcacctga gaggtgacgc taccggtacc cacgatagca
    88741 ggcagttcca gagaacgcgc catgatagag gtgtgggaag tacggccacc cgcgtcggtg
    88801 atgaaaccca gcaccttctt caggttcagc tgtgcggttt cggacggcgt caggtcagcg
    88861 gcaaccagaa tgacttcatc ctgaatggcg ctcaggtcga taatcttcag gcccaggatg
    88921 ttgcgcagca ggcgcttacc gatatcacgt acgtcagccg cacgttcttt caggtattca
    88981 tcatccagct cttccagggc agaagcctga ccttcgataa cttcatgagc agctgcgtca
    89041 gctgtcatgt gtttatcttt aatcagggct atgatttcct gctccagctc ctcatcttcg
    89101 agcagcataa tatgcccttc aaagatggct tctttttctt caccgaacgt ttcaccagct
    89161 ttcgttttga tcgtttccag ctgggctgat gccttggcac gaccgctcag aaaacgttca
    89221 acttcctgag caacctggtc ggcagaaatt tttttccggt caatgacaat ttcgtcttct
    89281 ttcagaagca gagctttacc gaaagcgata cccggggatg ctaaaatgcc tgaaatcata
    89341 accctacctt acttgtgact gatttttaaa agaacccggg aaattactcg agttccgcca
    89401 tcagtttaac cagatgttca accgctttct gctcgtcttc gccttctgcg gagatagtca
    89461 caacggtacc ttgagtcagg cccagagtct gcagtttaaa caggcttttc gcgctggcgc
    89521 ttttgccgtt ggaagtcaca gtaatttcag aagtgaagcc cttagcttct tttacaaact
    89581 gggcagcagg gcgggtgtgc agaccgttcg gagcggtaat ggtaacttct tgctggaaca
    89641 ttgtatttcc ccaacttata ggtttagtgt tgtggaacta aagtctagcc tggcggttcg
    89701 actttagcct gtattgttag cgccagtttt taacagacgc gacgcacgaa gagcgcaata
    89761 cagtccattg ctggcgggtg ttgaccgctg acgtttcgta cgtcattaaa cattatgccg
    89821 ctaaaggaag aattgaacca aatcataaaa tcgattcagc tttggctttt cctgtaacga
    89881 ttaatttcgc gcatcaaaat aattagtctg gttaaatacc agttccgaca ggctgaatca
    89941 atgccaggag gggcgaaagt ttgaagcagg ccacaaaaaa gcacctaaaa aggtgctttt
    90001 ttacgcattt ttaacaagct ggcattactg ttgcaattct ttctcagtga agagatcggc
    90061 aaacaatgcg gtgcttaaat aacgctcacc cgatgatggt agaataacca caatattctt
    90121 gttggtaaag ctttcatctt cttgtagttt caacgctgcg gcaacagctg ctccagaaga
    90181 gatacctgca agaatacctt cttcttccat tagacgacgg gcggtagaaa tggcttcttc
    90241 attggtgatg ccaatgactt tatcgaccag cttgagatcg aggttagccg ggataaaacc
    90301 agcgccaata ccctgaattt tatgcgggcc aggtttaatc tcttcacctg ccagcacctg
    90361 ggcgataact ggagaatcgg ttggctcaac ggcgacagag ataagatcgg tcttgccttt
    90421 ggtgccttta atgtagcggc tgacgccagt cagcgtaccg ccagtcccaa cgccagcaat
    90481 aaatacatca acctgaccgt cggtatcttc ccagatctcc ggaccggtgg ttttttcgtg
    90541 aatttcaggg tttgccggat tgctgaattg ttgcagcagc aggtatttct ctggattgct
    90601 ggcgacaatt tcttctgctt tttggattgc gcctttcatg cctttagcac cttccgtcag
    90661 caccaggttt gcacctaacg ctttcagcag cttgcggcgt tcaatactca tggtttctgg
    90721 catggtcagg gtgagtttgt aaccgcgagc ggcagctaca taggccagtg caatcccggt
    90781 attaccgctg gtcggttcaa ccagttcaac gcctggtttc agcacgccgc gcttttcggc
    90841 atcccaaatc atgttggcac cgatacggca cttaacgctg aagctggggt tacgagattc
    90901 caccttcgcc agaatgcgtc cgttaccgat gcgattcagg cgaaccagcg gcgtgtgacc
    90961 gatagtcagc gagttatctt caaaaatctt actcatggcc tgtccttaac tgtatgaaat
    91021 tgggatacaa caggtagcat acccgctcag agaatatgcg gaagtaagga tttagcatat
    91081 ctatatacag aagggaaata atgacatgca agatggaata aggggcggca taagccaccc
    91141 ctgtttcaca caaacggttt acaaaataac cggtagattg ttaccgccac atcgcgtgtt
    91201 tatcgcgata gcaatcgacc cacatcgccg tcgcgccaca aacggcaacg ggcatgatga
    91261 acagattaag cagcgggatc atcgtaaaca ggctggttaa agcaccaaac tgcatattgg
    91321 tgattttgcg tgtgcgcagg gcggtgcgca tctctttaaa cggcactttg tggttatcga
    91381 aggggtaatc gcaatactgg atggctaaca tccaggcgct aaacaggaac cacagtaccg
    91441 gcgcgacggt ttgcccaata ccagggatga agtaaagaat tagtaataca attgcgcgcg
    91501 gcagatacca gacaaatttt tgccattcgc gtttcatgat tcgcggcaca tctttcatga
    91561 taccgaaaat cccggtatct ggcggtgtag cgccagtcaa tcgtgcttcc agttgttcag
    91621 ccaataaacc gttaaacgga gcggcaatcc agttagcaat cgtggagaag aaatagccaa
    91681 acactaacag cacagagatg acagccagag gccacaacag ataactcagc cattgtagcc
    91741 agtccggaac gtaactcatg aaagtcggga tccagacatc gagctgtgta aagagccacc
    91801 agaatgcgcc ccccatcaac aaaatattga ccagcagcgg taaaataacg aaacgccgaa
    91861 tcccaggttg cgagacgagc ttccagcctt gcgcaaaata gtaaaaaccg ctgcgtgggg
    91921 cagatgtgaa tgatgaaacc ataatcagga tgagctcctt ttgaccaatc ccaggaaaat
    91981 tctgcgtatt ttaccgggta attgcgcaat ggacagttag gatatgttcg aaaaaacagc
    92041 aaaaagcacg atttcatcta tctttgtgct gtgaaagtta atagtgcact tgcacttgag
    92101 gtaatcggca aatactctta gtgagtaaat gtttgccgtg gtggcaaggt gttagaacaa
    92161 cagagaatat aatgatgcag gatttgcgtc tgatattaat cattgttggc gcgatcgcca
    92221 taatcgcttt actggtacat ggtttctgga ccagccgtaa agaacgatct tctatgttcc
    92281 gcgatcggcc attaaaacga atgaagtcaa aacgtgacga cgattcttat gacgaggatg
    92341 tcgaagatga tgagggcgtt ggtgaggttc gtgttcaccg cgtgaatcat gccccggcta
    92401 acgctcagga gcatgaggct gctcgtccgt cgccgcaaca ccagtaccaa ccgccttatg
    92461 cgtctgcgca gccgcgtcaa ccggtccagc agccagtttc gccacaggtc gcgccagcgc
    92521 cgcagccagt gcattcagca ccgcaaccgg cacaacaggc tttccagcct gcagaacccg
    92581 tagcggcacc acagcctgag cctgtagcgg aaccggctcc agttatggat aaaccgaagc
    92641 gcaaagaagc ggtgattatc atgaacgtcg cggcgcatca cggtagcgag ctaaacggtg
    92701 aactgcttct taacagcatt caacaagcgg gcttcatttt tggcgatatg aatatttacc
    92761 atcgtcatct tagcccggat ggcagcggcc cggcgttatt cagcctggcg aatatggtga
    92821 aaccgggaac ctttgatcct gaaatgaagg atttcactac tccgggtgtc actatcttta
    92881 tgcaggtacc gtcttacggt gacgagctgc agaacttcaa gctgatgctg caatctgcgc
    92941 agcatattgc cgatgaagtg ggcggtgtcg tgcttgacga tcagcgcagt atgatgactc
    93001 cgcagaaatt gcgcgagtac caggacatca tccgcgaagt caaagacgcc aacgcctgat
    93061 acacttaagg caaattaact cctcttcgaa cccccgcttg tcgggggttt ttagcattga
    93121 tggtgcgata tggaatcaat cgaacaacaa ctgacagaac tgcgaacgac gctttgccat
    93181 catgaatatc tttatcatgt gatggatgcg ccggaaattc ccgacgctga atacgacagg
    93241 ctgatgcgcg aactgcgcga gctggaaacc aaacatccag aactgattac gcctgattcg
    93301 cctacccaac gtgtaggtgc tgcgccgctg gcagctttca gccagatacg ccatgaagta
    93361 ccaatgctgt cactggataa cgtttttgat gaagaaagct ttcttgcttt caacaaacgt
    93421 gtgcaggacc gtctgaaaaa caacgagaaa gtcacctggt gctgtgagct gaagctggat
    93481 ggccttgccg tcagtattct gtatgaaaat ggcgttttag tcagtgccgc gacccgtggc
    93541 gatggcacca ccggggaaga tatcacgtct aatgtgcgta ctattcgcgc cattccgctg
    93601 aagctgcacg gagagaatat cccggcgcgt ctggaagtgc gtggtgaagt gttcctgccg
    93661 caggcggggt tcgaaaagat taacgaagat gcgcgacgca cgggcgggaa agtttttgct
    93721 aacccacgta atgcggcagc tggttcactg cgtcagcttg atccgcgtat tacagcgaag
    93781 cgaccgctca cttttttctg ctatggcgtt ggtgttctgg aagggggcga gctgccggat
    93841 actcatcttg gccgtttact gcaatttaaa aagtgggggt tgccggtcag cgatcgggta
    93901 acgctttgtg aatcggcgga agaagtgctg gcgttctatc acaaagtgga agaagaccgc
    93961 ccgacgctgg gctttgatat cgacggcgtg gtgattaagg tcaactcact ggaacagcag
    94021 gagcagcttg gctttgtcgc gcgtgccccg cgctgggcgg tagcgtttaa attcccggcg
    94081 caggagcaga tgacctttgt gcgtgacgtc gagtttcagg ttgggcgtac tggcgcgatt
    94141 acgcctgttg cgcgtctgga acctgtccat gttgctggcg tgctggtgag taacgcaacc
    94201 ttacacaatg cggatgaaat cgaacgtctt ggtttacgca ttggcgataa agtggtgatt
    94261 cgccgcgctg gcgacgtgat cccgcaggtg gttaacgtcg tgctttctga acgcccggaa
    94321 gatacccgtg aggttgtatt cccgacgcat tgtccggtat gtggttctga cgttgagcgt
    94381 gtggaaggtg aagcggttgc ccgctgtacc ggtggcctga tttgcggtgc gcagcgtaaa
    94441 gagtcgctga aacactttgt ttcccgccgt gcgatggatg ttgacggaat gggcgacaaa
    94501 atcatcgatc agctggttga aaaagaatat gtccatactc cggcagatct gttcaaactc
    94561 accgcaggca aactgaccgg actggagcgt atggggccaa aattggcaca aaacgtggtt
    94621 aacgcgctgg aaaaagcgaa agaaaccacc tttgctcgct tcctctatgc acttggcatc
    94681 cgtgaagtcg gcgaggccac cgcagcaggt ctggcggcat atttcggcac gctggaagcg
    94741 ctggaagccg cttcgattga agagctgcaa aaggtgcctg atgttggcat tgtcgttgca
    94801 tcccacgttc acaacttctt tgccgaagaa agcaaccgca atgtcatcag cgagctgttg
    94861 gcggaaggtg ttcactggcc tgcgccgatc gttatcaacg cggaagagat tgacagcccg
    94921 tttgctggta aaaccgtggt gcttacgggc agcttaagcc agatgtcgcg tgatgacgct
    94981 aaagctcgac tggtcgaact gggcgcgaaa gtcgcgggca gcgtgtcgaa gaaaaccgat
    95041 ctggtgatag cgggtgaagc tgcaggatct aaactggcga aggcgcagga actgggcatt
    95101 gaagtcatcg acgaagcgga aatgctgcgt ttgctgggta gctgagatgg aaaaagagca
    95161 gctgattgaa atagccaata cgataatgcc gtttggcaaa tacaaagggc gtcgcttaat
    95221 tgacctgccg gaggagtatc tgctgtggtt tgcccgcaaa gatgagttcc cggcagggaa
    95281 gctcggtgag ctaatgcaaa tcacgctgct gattaaaacc gaggggctga cgcaactggt
    95341 ccagcccctg aagcgtccgc tttaagcttt atcggcgctg ctttcctgct gcgcctgtaa
    95401 ctgttcggtc tggcgtttgt atcgacgcgc tagcaccgca cagaccatca attggatctg
    95461 atggaaaatc atcaggggca gcaccatcat accgatcacc gatgtgggga acagaatgtt
    95521 tgccatcggg atgccatttg ccagactctt tttcgaacca caaaagacga tagtaatttc
    95581 atctgcctta ttgaagccca gtcggcgtgc cataaagacg ttaactacaa tcacgatagc
    95641 cagaagaacg cagctgacca ccacgataaa cagcaatgat ccccagccaa ctttatgcca
    95701 gataccatta acgacggctt cgctgaacgc tgtataaacc accaacagaa tggacgtctg
    95761 gtcagttttc gcaatccatt ttttattgcg cgacacccag tcaccaatcc acggccggga
    95821 aagatgcccc aacacaaacg gcagcagcag ttgcagcata attttaccga cctgctcaag
    95881 gctgcccctt gcaccgtgaa cgttcatcac cagaccaacc agcaatggtg aaaggaaaat
    95941 ccccagcagg ctggatgctg acgcagaaca aaccgccgcc gcgacgttac cgcccgccat
    96001 tgacgtgaag gcgattgcag actgcacggt agccgggaga atgcacaagt agagaaaacc
    96061 ggagtagagc atcgggtcga catttaccgg tttccaccag gcaaacagta cacccagaat
    96121 cggaaacagc acgaaggtgc tgcacattac ccacaaatgc agtcgccagt gaccaccgcc
    96181 agcaataatc gcctcacgcg acaacttcgc gccgtgcata aagaacagca gggcaatagc
    96241 tgcggtggtc agattttcaa agaaggggac gaaatcgcct ctggccggaa agaaagaggc
    96301 cagcaacacc accgtgatca gggttaaggt gaaaggatcg aggatacgaa aaagtttcat
    96361 aaagactcca gatgatcgat ggttctattg tgcttcttcc agtttgagaa ataaaattga
    96421 tttattgcat ccattcatga agaaaacaga tgaattattc tttaaaacaa ttaaaggttt
    96481 tcgtcacagt agcgcaggag aaaagtttta gtcgtgcagg agagcgtatc ggcttgagcc
    96541 agtcggcagt aagtcacagt gtgaaggagc tggaaaatca tactggtgtt cgcctgctgg
    96601 acagaaccac gcgtgaagtg gtgcttacag atgcagggca gcagttggct ttgcgtcttg
    96661 agcgactgct ggatgaactt aacagcacgt tgcgcgatac cgggcgtatg gggcaacaac
    96721 tgagcggaaa agttcgggtc gctgccagcc agaccatttc cgcgcatctt attccgcaat
    96781 gcattgctga aagccatcgc cgctatccag atattcagtt tgtcctgcat gatcgtccgc
    96841 agcagtgggt gatggaaagt attcgccagg gagatgtcga ctttggcatc gtcatcgatc
    96901 caggcccagt gggcgatctg caatgtgaag cgattctttc cgagcctttc tttctgcttt
    96961 gccatcgcga tagcgctttg gccgtggaag attatgtacc ctggcaggca ttacagggag
    97021 cgaaattggt gttgcaggat tacgcgtcag gcagccgacc gctgattgac gcagcgctgg
    97081 cgcgcaatgg tattcaggcg aatattgtgc aggagattgg acatccggcg acgctgttcc
    97141 cgatggtagc cgcaggcatc ggcatcagta ttcttcctgc actggcgcta ccattgcctg
    97201 aaggtagccc actggtggtt aagcgcatta cgccggtggt tgaacgccag ttgatgctgg
    97261 tgcgcaggaa gaaccgttca ctttccaccg ccgctgaagc gttatgggac gtggtacgag
    97321 accagggcaa tgcgctaatg gctggtcggg aaggggatcc gttgtatcag atatagatat
    97381 cgatatgagt cgttttttca acaggattta gaagggatac attcagagac aaacgctgct
    97441 ctttctcctg cgccttttct gcctgctgtt tctgcaactg cgccagttgt gtttccagta
    97501 gggtgatttg ctgttgaatc aattcggcct gtttttgctt gtcttcagca ctgccgctag
    97561 tgtctacaat ctctttaatt tgttgagtga gtttgatgat ttgctgactt atccgcgcga
    97621 tttgtgaagc aacatcgcta ccttgtgatg ccctgttact actctgtgtt tgtatggaag
    97681 gaattgaagt tgaagagata gtggccattg ttcagtcctc gtcactgtac gaggtaatat
    97741 cggtagaaga acaaggaact tgagaagaaa aatcaatctt acaaatcgca gatagcaaaa
    97801 aagcgccttt agggcgcttt tttgcattgg tgggtcgtgc aggatgactc gcttcgctcg
    97861 cccttcgggc cgtcgccgcg agcggctccg ttgtttcgct gcgctcaact caaacctgct
    97921 gcaggttcga atctttgagt atcgcagata g
//
LOCUS       DADMGC010000003        69005 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.003, whole genome shotgun sequence.
ACCESSION   DADMGC010000003 DADMGC010000000
VERSION     DADMGC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 69005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.003"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..188
                     /locus_tag="JNZ73_000200"
     CDS             <1..188
                     /locus_tag="JNZ73_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8730618.1"
                     /translation="GIRIENLGGGVANIPFQNGILPVDPSGHGTVNMRAWPVNLVGGE
                     LETGKFQGTATITVMVR"
     gene            complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="JNZ73_000201"
     CDS             complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="JNZ73_000201"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAY8730619.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1156..3192
                     /locus_tag="JNZ73_000202"
     CDS             1156..3192
                     /locus_tag="JNZ73_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY8730620.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLALQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLSVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3150..3545
                     /locus_tag="JNZ73_000203"
     CDS             3150..3545
                     /locus_tag="JNZ73_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAY8730621.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            3565..4155
                     /gene="diaA"
                     /locus_tag="JNZ73_000204"
     CDS             3565..4155
                     /gene="diaA"
                     /locus_tag="JNZ73_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HAY8730622.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4165..4740
                     /gene="yraP"
                     /locus_tag="JNZ73_000205"
     CDS             4165..4740
                     /gene="yraP"
                     /locus_tag="JNZ73_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HAY8730623.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(4854..5894)
                     /locus_tag="JNZ73_000206"
     CDS             complement(4854..5894)
                     /locus_tag="JNZ73_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY8730624.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLPGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(5967..6602)
                     /locus_tag="JNZ73_000207"
     CDS             complement(5967..6602)
                     /locus_tag="JNZ73_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAY8730625.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6730..7248
                     /gene="yhbO"
                     /locus_tag="JNZ73_000208"
     CDS             6730..7248
                     /gene="yhbO"
                     /locus_tag="JNZ73_000208"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HAY8730626.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7228..7671)
                     /locus_tag="JNZ73_000209"
     CDS             complement(7228..7671)
                     /locus_tag="JNZ73_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HAY8730627.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            7722..8024
                     /gene="yhbQ"
                     /locus_tag="JNZ73_000210"
     CDS             7722..8024
                     /gene="yhbQ"
                     /locus_tag="JNZ73_000210"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HAY8730628.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8011..8514)
                     /locus_tag="JNZ73_000211"
     CDS             complement(8011..8514)
                     /locus_tag="JNZ73_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8730629.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8508..9032)
                     /locus_tag="JNZ73_000212"
     CDS             complement(8508..9032)
                     /locus_tag="JNZ73_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HAY8730630.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVCDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9241..10236
                     /locus_tag="JNZ73_000213"
     CDS             9241..10236
                     /locus_tag="JNZ73_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY8730631.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHISINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10245..11123
                     /locus_tag="JNZ73_000214"
     CDS             10245..11123
                     /locus_tag="JNZ73_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY8730632.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            11204..12211
                     /locus_tag="JNZ73_000215"
     CDS             11204..12211
                     /locus_tag="JNZ73_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAY8730633.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12329..13573)
                     /gene="mtr"
                     /locus_tag="JNZ73_000216"
     CDS             complement(12329..13573)
                     /gene="mtr"
                     /locus_tag="JNZ73_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAY8730634.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13727..15616)
                     /locus_tag="JNZ73_000217"
     CDS             complement(13727..15616)
                     /locus_tag="JNZ73_000217"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HAY8730635.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGELRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15609..15689)
                     /gene="yrbN"
                     /locus_tag="JNZ73_000218"
     CDS             complement(15609..15689)
                     /gene="yrbN"
                     /locus_tag="JNZ73_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HAY8730636.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(15796..16680)
                     /gene="nlpI"
                     /locus_tag="JNZ73_000219"
     CDS             complement(15796..16680)
                     /gene="nlpI"
                     /locus_tag="JNZ73_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HAY8730637.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEHTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(16789..18924)
                     /gene="pnp"
                     /locus_tag="JNZ73_000220"
     CDS             complement(16789..18924)
                     /gene="pnp"
                     /locus_tag="JNZ73_000220"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAY8730638.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19171..19440)
                     /gene="rpsO"
                     /locus_tag="JNZ73_000221"
     CDS             complement(19171..19440)
                     /gene="rpsO"
                     /locus_tag="JNZ73_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAY8730639.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(19589..20533)
                     /gene="truB"
                     /locus_tag="JNZ73_000222"
     CDS             complement(19589..20533)
                     /gene="truB"
                     /locus_tag="JNZ73_000222"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAY8730640.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20533..20934)
                     /gene="rbfA"
                     /locus_tag="JNZ73_000223"
     CDS             complement(20533..20934)
                     /gene="rbfA"
                     /locus_tag="JNZ73_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAY8730641.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21098..23746)
                     /gene="infB"
                     /locus_tag="JNZ73_000224"
     CDS             complement(21098..23746)
                     /gene="infB"
                     /locus_tag="JNZ73_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAY8730642.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(23771..25258)
                     /gene="nusA"
                     /locus_tag="JNZ73_000225"
     CDS             complement(23771..25258)
                     /gene="nusA"
                     /locus_tag="JNZ73_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAY8730643.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(25286..25738)
                     /gene="rimP"
                     /locus_tag="JNZ73_000226"
     CDS             complement(25286..25738)
                     /gene="rimP"
                     /locus_tag="JNZ73_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAY8730644.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(25945..26021)
                     /locus_tag="JNZ73_000227"
     tRNA            complement(25945..26021)
                     /locus_tag="JNZ73_000227"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25985..25987),aa:Met,seq:cat)
     gene            26369..27712
                     /gene="argG"
                     /locus_tag="JNZ73_000228"
     CDS             26369..27712
                     /gene="argG"
                     /locus_tag="JNZ73_000228"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAY8730645.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(27720..29345)
                     /locus_tag="JNZ73_000229"
                     /pseudo
     CDS             complement(27720..29345)
                     /locus_tag="JNZ73_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(29804..29890)
                     /locus_tag="JNZ73_000230"
     tRNA            complement(29804..29890)
                     /locus_tag="JNZ73_000230"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29854..29856),aa:Leu,seq:gag)
     gene            complement(29905..30237)
                     /gene="secG"
                     /locus_tag="JNZ73_000231"
     CDS             complement(29905..30237)
                     /gene="secG"
                     /locus_tag="JNZ73_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAY8730646.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30465..31802)
                     /gene="glmM"
                     /locus_tag="JNZ73_000232"
     CDS             complement(30465..31802)
                     /gene="glmM"
                     /locus_tag="JNZ73_000232"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAY8730647.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRVLQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(31795..32643)
                     /gene="folP"
                     /locus_tag="JNZ73_000233"
     CDS             complement(31795..32643)
                     /gene="folP"
                     /locus_tag="JNZ73_000233"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAY8730648.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(32733..34667)
                     /gene="ftsH"
                     /locus_tag="JNZ73_000234"
     CDS             complement(32733..34667)
                     /gene="ftsH"
                     /locus_tag="JNZ73_000234"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAY8730649.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(34767..35396)
                     /gene="rlmE"
                     /locus_tag="JNZ73_000235"
     CDS             complement(34767..35396)
                     /gene="rlmE"
                     /locus_tag="JNZ73_000235"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HAY8730650.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35522..35815
                     /gene="yhbY"
                     /locus_tag="JNZ73_000236"
     CDS             35522..35815
                     /gene="yhbY"
                     /locus_tag="JNZ73_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAY8730651.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKKRKISLPR"
     gene            complement(35971..36447)
                     /gene="greA"
                     /locus_tag="JNZ73_000237"
     CDS             complement(35971..36447)
                     /gene="greA"
                     /locus_tag="JNZ73_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAY8730652.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36695..38128
                     /gene="dacB"
                     /locus_tag="JNZ73_000238"
     CDS             36695..38128
                     /gene="dacB"
                     /locus_tag="JNZ73_000238"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY8730653.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFGVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38314..39486)
                     /gene="cgtA"
                     /locus_tag="JNZ73_000239"
     CDS             complement(38314..39486)
                     /gene="cgtA"
                     /locus_tag="JNZ73_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HAY8730654.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39502..40467)
                     /locus_tag="JNZ73_000240"
     CDS             complement(39502..40467)
                     /locus_tag="JNZ73_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY8730655.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40594..40851)
                     /gene="rpmA"
                     /locus_tag="JNZ73_000241"
     CDS             complement(40594..40851)
                     /gene="rpmA"
                     /locus_tag="JNZ73_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAY8730656.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40872..41183)
                     /gene="rplU"
                     /locus_tag="JNZ73_000242"
     CDS             complement(40872..41183)
                     /gene="rplU"
                     /locus_tag="JNZ73_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAY8730657.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41442..42413
                     /gene="ispB"
                     /locus_tag="JNZ73_000243"
     CDS             41442..42413
                     /gene="ispB"
                     /locus_tag="JNZ73_000243"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HAY8730658.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42641..42919
                     /gene="sfsB"
                     /locus_tag="JNZ73_000244"
     CDS             42641..42919
                     /gene="sfsB"
                     /locus_tag="JNZ73_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HAY8730659.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(42967..44226)
                     /gene="murA"
                     /locus_tag="JNZ73_000245"
     CDS             complement(42967..44226)
                     /gene="murA"
                     /locus_tag="JNZ73_000245"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAY8730660.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(44281..44535)
                     /gene="ibaG"
                     /locus_tag="JNZ73_000246"
     CDS             complement(44281..44535)
                     /gene="ibaG"
                     /locus_tag="JNZ73_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HAY8730661.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44695..44988)
                     /gene="mlaB"
                     /locus_tag="JNZ73_000247"
     CDS             complement(44695..44988)
                     /gene="mlaB"
                     /locus_tag="JNZ73_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HAY8730662.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(44988..45623)
                     /gene="mlaC"
                     /locus_tag="JNZ73_000248"
     CDS             complement(44988..45623)
                     /gene="mlaC"
                     /locus_tag="JNZ73_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HAY8730663.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45642..46193)
                     /gene="mlaD"
                     /locus_tag="JNZ73_000249"
     CDS             complement(45642..46193)
                     /gene="mlaD"
                     /locus_tag="JNZ73_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HAY8730664.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46198..46980)
                     /gene="mlaE"
                     /locus_tag="JNZ73_000250"
     CDS             complement(46198..46980)
                     /gene="mlaE"
                     /locus_tag="JNZ73_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HAY8730665.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(46988..47797)
                     /gene="mlaF"
                     /locus_tag="JNZ73_000251"
     CDS             complement(46988..47797)
                     /gene="mlaF"
                     /locus_tag="JNZ73_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HAY8730666.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48007..48972
                     /locus_tag="JNZ73_000252"
     CDS             48007..48972
                     /locus_tag="JNZ73_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAY8730667.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIIWLAMLYWLSPILVE"
     gene            48986..49972
                     /gene="kdsD"
                     /locus_tag="JNZ73_000253"
     CDS             48986..49972
                     /gene="kdsD"
                     /locus_tag="JNZ73_000253"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HAY8730668.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            49993..50559
                     /gene="kdsC"
                     /locus_tag="JNZ73_000254"
     CDS             49993..50559
                     /gene="kdsC"
                     /locus_tag="JNZ73_000254"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HAY8730669.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDCPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50556..51131
                     /gene="lptC"
                     /locus_tag="JNZ73_000255"
     CDS             50556..51131
                     /gene="lptC"
                     /locus_tag="JNZ73_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HAY8730670.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51100..51657
                     /gene="lptA"
                     /locus_tag="JNZ73_000256"
     CDS             51100..51657
                     /gene="lptA"
                     /locus_tag="JNZ73_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HAY8730671.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51664..52389
                     /gene="lptB"
                     /locus_tag="JNZ73_000257"
     CDS             51664..52389
                     /gene="lptB"
                     /locus_tag="JNZ73_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HAY8730672.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52437..53870
                     /gene="rpoN"
                     /locus_tag="JNZ73_000258"
     CDS             52437..53870
                     /gene="rpoN"
                     /locus_tag="JNZ73_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAY8730673.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            53893..54180
                     /gene="hpf"
                     /locus_tag="JNZ73_000259"
     CDS             53893..54180
                     /gene="hpf"
                     /locus_tag="JNZ73_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HAY8730674.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54298..54789
                     /gene="ptsN"
                     /locus_tag="JNZ73_000260"
     CDS             54298..54789
                     /gene="ptsN"
                     /locus_tag="JNZ73_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HAY8730675.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            54835..55689
                     /gene="rapZ"
                     /locus_tag="JNZ73_000261"
     CDS             54835..55689
                     /gene="rapZ"
                     /locus_tag="JNZ73_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAY8730676.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55686..55958
                     /gene="npr"
                     /locus_tag="JNZ73_000262"
     CDS             55686..55958
                     /gene="npr"
                     /locus_tag="JNZ73_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HAY8730677.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56171..56803
                     /gene="yrbL"
                     /locus_tag="JNZ73_000263"
     CDS             56171..56803
                     /gene="yrbL"
                     /locus_tag="JNZ73_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HAY8730678.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(56800..57528)
                     /gene="mtgA"
                     /locus_tag="JNZ73_000264"
     CDS             complement(56800..57528)
                     /gene="mtgA"
                     /locus_tag="JNZ73_000264"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HAY8730679.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSTPDGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57525..58178)
                     /gene="elbB"
                     /locus_tag="JNZ73_000265"
     CDS             complement(57525..58178)
                     /gene="elbB"
                     /locus_tag="JNZ73_000265"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HAY8730680.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58408..60744)
                     /gene="arcB"
                     /locus_tag="JNZ73_000266"
     CDS             complement(58408..60744)
                     /gene="arcB"
                     /locus_tag="JNZ73_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HAY8730681.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEKSTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60840..61769)
                     /locus_tag="JNZ73_000267"
     CDS             complement(60840..61769)
                     /locus_tag="JNZ73_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAY8730682.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62444..66904
                     /gene="gltB"
                     /locus_tag="JNZ73_000268"
     CDS             62444..66904
                     /gene="gltB"
                     /locus_tag="JNZ73_000268"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HAY8730683.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPTTTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIHPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            66917..68335
                     /locus_tag="JNZ73_000269"
     CDS             66917..68335
                     /locus_tag="JNZ73_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY8730684.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68519..68998
                     /locus_tag="JNZ73_000270"
     CDS             68519..68998
                     /locus_tag="JNZ73_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HAY8730685.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        16854 a        18752 c        17148 g        16251 t
ORIGIN      
        1 tcggtattcg tattgaaaat ctcggcggcg gagttgcaaa tattcctttt cagaacggta
       61 tccttcccgt tgatccttcc gggcatggca ccgtcaatat gcgcgcctgg ccagttaatc
      121 tggtcggtgg tgagctggaa acaggaaaat ttcagggcac agccaccatt accgtcatgg
      181 tgcggtaaaa gaagacgttg cgttttgatt aacgcaacgc ttcggcactg ttacccctgc
      241 tgctccagcg catacttata cagcgcattt ttcttcacgc catgaatttc tgcggccagc
      301 gccgccgctt ttttcagcgg cagttctgcc tgtagcagcg ccagcgtgcg cagggcatcg
      361 gcgggtaagt cttcttcctg tgctttatga ccttcgacaa tcagcaccat ttcgcctttg
      421 cgacggtttt catcttcctt tacccacgcc agcagctcgc caacgggcgc gccgtgaatg
      481 gtttcccagg ttttggtcag ctcacgcgcc agaaccacgt agcgggattc gcctaatacc
      541 gcaacgatat cttccaggct atctaacaga cggtgggtag attcataaaa aatcagcgtg
      601 cgcggctccg cttcaatggc ttttagcgca tcacggcggc cttttgattt ggcaggtaaa
      661 aagccttcgt aacagaaacg gtcagatggt aaacccgcgg cgcttaacgc agtgatagca
      721 gcacacggtc cgggtagcgg caccacacga atccccgctt cacggcaggt acgcaccaga
      781 tggtagccag gatcgttaat tagcggcgtt ccggcatcgg aaaccagcgc aatgttttgc
      841 ccctcttgca gcttcgccag cagcgtttcg gctttttgtt gttcgttatg gtcgtgcagc
      901 gcaaacaacc gggcattaat cccaaaatgt tgcagcaata aaccggtgtg acgagtatcc
      961 tcggcggcaa tcagatcaac ggcctgtaat acctctaacg tacgctgggt gatatccgcc
     1021 agattgccga ttggcgtcgg tacaatgtaa agctggccct gagaattatc cgccgattgg
     1081 tgttgtttca ttgtgtcgtc cgtattgccg atttaatatt gagcattgcg taaaaaaaat
     1141 atcactggat acattatggt accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg
     1201 cctgttgttc tggcagccct gattttcgcc ggttgtggca cccatactcc cgatcagtcc
     1261 actgcttata tgcagggcac ggcgcaggct gattctgcct tttatcttca gcagatgcag
     1321 caaagctctg atgataccag gatcaactgg caattactcg ccattcgtgc actggtgaaa
     1381 gaaggtaaaa ccgggcaggc ggttgagttg tttaaccaac taccgcaaga actgaacgat
     1441 tctcagcgtc gcgagaaaac actgctggcg gcagagatta aactggcgca gaaagatttt
     1501 gctggcgcgc aaaacttgct ggcgaaaatc acacctgccg atttagaaca aaaccagcaa
     1561 gcgcgttact ggcaggcaaa aatcgatgcc agccaggggc gtccttccat tgatttactg
     1621 cgcgcgttaa ttgctcagga accgctgctt ggcgcgaaag aaaaacagca gaatattgat
     1681 gccacctggc aggcgctctc ctccatgact caggaacagg cgaatacgct ggtgatcaac
     1741 gccgacgaaa atattctgca aggctggctg gctctgcagc gcgtctggtt tgataaccgt
     1801 aacgatcccg acatgatgaa agccgggatc gccgactggc agaaacgtta tccgaacaat
     1861 ccgggcgcga aaatgctgcc aacgcagttg gttaacgtaa aagcgtttaa accagcctcg
     1921 accaacaaaa tcgccctgct gttgccgctg aatggccagg cagcggtatt tggtcgcact
     1981 attcagcaag gctttgaagc cgcgaaaaat atcggcactc agccagtggc ggctcaggta
     2041 gctgccgcac ctgccgcaga cgtagcagaa caacctcagc cgcaaactgc ggatggcgtt
     2101 gccagcccgg cacaagcctc ggttagcgat ctgaccggtg atcagcctgc ggcccagccg
     2161 gtgcctgtaa gcgccccggc gacaagcacc gcagcggtaa gcgcacccgc aaatccatcc
     2221 gcagagctga aaatctacga tacctcatca caaccactta gccagatctt aagccaggtt
     2281 cagcaggatg gcgcgagtat tgtggttggt ccgttgctga aaaataacgt tgaagagttg
     2341 ctgaagagca acaccccgct gagcgtactg gcactgaacc agccggagaa tatcgaaaac
     2401 cgcgtcaata tttgttactt cgcgctttca ccggaagacg aagcgcgcga tgcagcgcgt
     2461 catattcgtg accagggtaa acaagcgccg ctggtgctga tcccacgcag tgcattgggc
     2521 gatcgcgtag ccaatgcgtt tgcgcaagag tggcagaaac tgggcggcgg caccgttctg
     2581 caacaaaaat ttggttccac cagcgaatta cgcgcgggtg ttaacggcgg ttctggtatt
     2641 gctttaacgg gtagcccgat tactcccaga gcgacaaccg actccggcat gacgaccaac
     2701 aatccaacgc tgcaaaccac gccaaccgat gaccagttca ccaataatgg cggtcgtgtc
     2761 gatgcggtct acattgtggc aacgccgggt gaaatcgctt ttatcaaacc gatgatcgcc
     2821 atgcgtaacg gtagccagag cggtgcaacg ctgtacgcca gttcccgcag cgcacaaggc
     2881 accgcaggcc cggatttccg actggagatg gaaggcttgc agtacagcga aatcccgatg
     2941 ctggcaggcg gtaatctgcc gttaatgcag caggcgctca gcgcggtgaa caacgattat
     3001 tcgctggctc gcatgtatgc gatgggcgtt gatgcctggt cgttggcaaa tcatttctca
     3061 caaatgcgcc aggttcaggg ttttgaaatc aacggtaata ccggaagtct gacggctaac
     3121 ccggattgcg tgattaacag gaagttatca tggctacagt accaacaagg tcaggtagtc
     3181 cccgccagtt aaccaccaaa cagaccggcg atgcgtggga agcacaagcg cgtcgctggc
     3241 tggaaggcaa aggactgcgg tttatcgccg ctaacgtgaa cgagcgtggc ggcgagatcg
     3301 atctgataat gcgtgaaggc ctgaccaccg tttttgtcga ggtacgctat cgccgctctg
     3361 cgctttatgg cggcgcggca gccagtgtga cccgcagcaa acaacacaaa ttattacaga
     3421 ctgcccgctt gtggctcgcg cgtcataatg ggagttttga tactgtggat tgccggttcg
     3481 atgtggtagc cttcaccggg aatgaggttg agtggattaa ggatgccttt aatgaccact
     3541 cataattaag gtttaaggat tagcgtgcaa gaaagaatta aagcttgctt cactgaaagc
     3601 attcaaactc aaattgcggc ggcagaggcg cttccggatg ccatctcccg tgcagccatg
     3661 acgctggttc agtctctgct caatggcaac aaaatcctct gttgtggtaa tggaacttcc
     3721 gctgccaatg cacagcattt tgctgccagc atgatcaacc gtttcgaaac ggagcggccc
     3781 agcttacctg ccattgcact aaatactgat aatgttgtct taacggcgat tgccaacgat
     3841 cgcttacatg atgaagtgta tgcaaaacag gtgcgggcgc tgggtcatgc gggagatgta
     3901 ttgttagcca tttccacccg tggcaacagc cgcgatattg ttaaagcagt tgaagccgcc
     3961 gttacgcgtg atatgaccat tgtggcattg accggctatg acggcggcga acttgcaggt
     4021 ttgttagggc cacaggatgt ggagatccgc attccttcgc atcgtagtgc tcgcattcag
     4081 gaaatgcata tgctgacggt aaattgcctg tgcgatctga tcgataacac gcttttccct
     4141 caccaggatg attaaggaga atacatgaag gcattatcgc caatcgcagt ccttatttcc
     4201 gcgctgctgt tgcaaggttg tgttgccgct gccgtagtgg gtaccgctgc tgtgggtacc
     4261 aaagccgcaa ctgacccacg cagtgtcggc acccaggtgg acgatggtac cctggaagtg
     4321 cgcgtgaaca gcgcattgtc gaaagacgaa cagattaaga aagaaacccg tattaatgtc
     4381 acggcctatc agggcaaagt gctgctggtt gggcagtcac caaatgctga actttcggct
     4441 cgcgccaaac agattgctat gggcgtagac ggtgccaacg aagtgtataa cgagattcgt
     4501 cagggccagc cgattggtct gggcgaagca tctaacgata cgtggatcac caccaaagtg
     4561 cgttcgcagc tcttaaccag cgacctggtg aaatcgtcca acgtgaaagt gaccaccgaa
     4621 aacggtgaag tgttcctgat ggggctggta actgaacgtg aagcgaaagc ggcggcagat
     4681 attgccagcc gggtgagcgg cgtaaagcgg gtaaccacag cgtttacgtt tattaaatag
     4741 cagccccttg taatgcctga tgcgacgctt gccgcgtctt atcaggccta caacgatgca
     4801 aaccgtaggt cggataaggc gtttacgccg catccgacat gttttccctc aaatcagaac
     4861 aacagcgcca gcccgccgac aatcacacca gacactgcca ccatcgcccc tgtcagccat
     4921 aaggctttcg ccgggaacgc tttgcgcagc ataatcagtg acggcaaact cactgccggg
     4981 agcgtcatca acaatgccag cgccggagcg gttcccatac ctgccagcat catcgtttgt
     5041 acaatcggaa tttctgctgc cgtgggaatg acaaacaagc atcctgctac cgccatcgcc
     5101 accacccaca tcaggctgtt atcgacagca ccatcggcat gggggaataa ccagacgcgt
     5161 gcggcaccca acaccagtac tgcaaggatg taaaccggga tcgtactcca gaaaagtgtc
     5221 catagcgccc tgccccagcg gctaaaaaaa ccgctctgtg cttccggtat gtcaatttcg
     5281 accggtgcct gcgtttgcgg tgtttcacgc acccattttt gcaccagcgt cgcaatcagc
     5341 aacaccatca ccagcccggc caccagacga atcgccgcaa aaccccagcc gaggacaaac
     5401 cccataaaca ccagcgtcgc cgggtttaac accggattgc ccatccagaa tgccagcgca
     5461 ccgcccatcg atacctgttg gcgacgcatt cccgccgcga ccggagccgc acagcaggta
     5521 cacatcatgc ccggcagcga aaacagcgtt cccggcagcg tgccgcgaaa gcgcgattgc
     5581 ccaagcgtac gcaacaacca gtcacgcggg atcagcacct gaatcaacga accgaggatc
     5641 acccccagca ccgccgcttt ccatactgcg aggaaataga tcatcgcgta atccaacgcc
     5701 gcttgccacg gattagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg
     5761 gctgcagtaa acgctttgcc gtagtaaggt tgccatttca cataccagag accggcaatg
     5821 acaacgagaa agaaaagcgc gggtttccac cactgaatgg gcgttgccgc ctgagatgaa
     5881 gactgaccag tcatagcatt ccccaggaat aagttatgat aattgagcgc gtgaatatta
     5941 cgctcactat caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt
     6001 aaaatcgtca cgccttcatg ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg
     6061 tctctcgcat caatggattt ccagttacct ggtaacaaac ggaacagcgg cgcaaaaagc
     6121 gtttcgttca tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc
     6181 aatttcggcc agttctgggc aattaatgct tcttccatct cccctttgac gcggttatag
     6241 aaaaacggcg agtgggcatt ggcacccata gcactgacca ccagcatatg ctgcgcgccc
     6301 agtcgccgcc cggttaatgc ggtatccact accagcgtgt aatcggcatg aataaacgcc
     6361 tctttgctcc ccgcttctcg ccgcgtggtg ccgagacaac aaaatacaat gtcgatgggt
     6421 tcggtgacct gcgccaacgc gtcactcagt tgcggatcat ggggattaaa caccccgggc
     6481 atatcgccca acggacgtcg cgtcggcgca gcaatggcgt taactttcgg ttcattaatc
     6541 aacatccgca gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag tacctgactc
     6601 atcttcgctc ctttacagaa ttgtctgcct tgcgctcccc ggctcatgca ccagtcttaa
     6661 tagaccatga ggtaattatc cccgattgtg gaaaattcgc ctcatccaat gcaacaacgc
     6721 ggaggaagca tgagtaagaa aattgccgtt ttaatcactg atgaatttga agattcagaa
     6781 tttacttcac ccgcagacga gttccgtaaa gccggacacg aagtgattac tattgaaaaa
     6841 caagcgggta aaacggtgaa aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc
     6901 atcgatgaag tgacgccagc ggagtttgat gccctgttgc taccgggtgg tcattcaccg
     6961 gattatctgc gtggcgacaa ccgttttgtc acctttaccc gtgattttgt gaatagcggc
     7021 aaacctgttt tcgccatctg tcacggtccg cagctgctga tcagcgccga tgtgattcgg
     7081 gggcgcaaac tgaccgcagt taaaccgatc attattgatg ttaaaaatgc gggcgtggaa
     7141 ttttacgatc aggaagtcgt ggttgataaa gatcagctgg ttaccagccg gacaccggac
     7201 gatctgccag cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagt cgcgtagcca
     7261 aatcattttt ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat
     7321 ttcccacacc ggggccgaca gcattctggc aaccggaaag cgacgattgt acgccttgcg
     7381 cgcgaggtcg ctttcctcac cttccagcct gcggatctca cctttaaact gcacaccgcg
     7441 aattaacgct accgttttcg gctgaccgtt taccgttccg gcaaccgcag cctgcggccc
     7501 actcatctgc gcgtggcgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc
     7561 atcaaaaaga taaaaggcat tagcgcacca gagttcccct tcctgctgca cacaccaggt
     7621 gacaacatgt tgttttgcca gccaacggct gatggcgatg agtgtttcca ttgctgttct
     7681 cttttatact gtgggcctga ccttaacatg cgatgcagac gatgaccccc tggtttcttt
     7741 acttgatccg taccgccgac aataagcttt ataccgggat caccacggat gtcgaacgcc
     7801 gctatcagca gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga
     7861 cgctggcgtt ttctgcgcca gtgggcgacc gttcgctggc actacgggcg gaatatcgcg
     7921 ttaagcaact gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca gggtttgcag
     7981 aactgttgag cagcctgcaa accccggaga ttaaaagcga ttgaaatgct cgtgatactc
     8041 aaccagaccg gttacgccat tcagcgcgtc atccgctaaa cgatgtacct ggaaggcgct
     8101 ttcagtgccc ggccagcggc aacgcagatc gtgatgcgcc gccagttcaa agccgaaacg
     8161 actgtacagc gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga
     8221 atcgagtcct tcatagacca gttggcgtgc cagcccctgt ccacggtatt tttcatcaac
     8281 cgccaacggt gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa
     8341 tgccacatag ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa
     8401 gccatcttca cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgagcgacg
     8461 cagcagggca tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca
     8521 cctaccgatg tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc
     8581 agttgcagca gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc
     8641 aggtttttca catacacccc cagctccgta tcgccttcaa tcaccagccg acgctggaag
     8701 aagagcgtat ccggatcttg tttacgcgcc gcaatcatca gcagatcgct ggcgtcggca
     8761 ctaaaactca catcagcttg cgcgttctgg ctgaccacca gtttgccatt caccaccgag
     8821 gtaaaccatt gcaggtcaat atcacacaca tgaatactta accagcggcc ttcaagaaac
     8881 tccagctcgc catcatccag cgcctgacgg aattgccagc ttaagacctg ctcaagaacc
     8941 tggcgtttta gcgcaaatgg cgtcagtttt accggtacac tcaacagaga tggccccaaa
     9001 tgcacaatac gggaacgcag tttatccaac acgagtttta ctccctgttt caacaatcat
     9061 cctattttgc catatcagaa aaataacata gcggtataaa tcaacaattc catatgaaat
     9121 tgctgctacc accaatacaa ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta
     9181 actaaaatcc cagttcgtta acatttttgc gttttgatag cgcaaccttc aggaaaaatt
     9241 atggagctgc tctgccctgc cggaaatctc ccggcgctta aggcggccat cgaaaacggc
     9301 gcagatgctg tttatatcgg gctaaaagat gataccaatg cccgtcactt cgccggcctt
     9361 aactttaccg agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa
     9421 cttcacatct cgattaacac ttttgcgcat ccggacggtt acgcccgttg gcagcgtgcc
     9481 gtggatatgg cggcgcagct gggtgccgac gcgctgatcc tcgccgacct cgccatgctg
     9541 gagtacgccg ccgagcgtta cccgcatatt gaacgtcatg tgtcagtgca ggcttcggcg
     9601 accaatgaag aggcgattaa cttttatcat cgccattttg acgttgcccg cgtggtgctg
     9661 ccgcgcgtgt tgtcgattca tcaggtgaaa cagctggcac gggttactcc tgtcccgctg
     9721 gaagtgtttg ctttcggcag cctgtgcatt atgtcggaag gccgttgcta tctgtcgtcg
     9781 tatctgacgg gtgagtcgcc caacactgtg ggtgcgtgtt ctccggcccg tttcgtgcgc
     9841 tggcaacaaa cgccgcaggg gctggaatcc cgcctgaacg aagtgctgat cgaccgttat
     9901 caggacggcg aaaacgcagg ttatccgacg ctgtgtaaag ggcgttatct ggtggacggc
     9961 gagcgctatc acgcgctgga agaaccaacc agtctcaata ccctggaact gctgccggag
    10021 ttaatggctg cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat
    10081 gtcagccagg tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa
    10141 aacttcgtac cgcaaagcgc gtggatggag acgctcgggt cgatgtccga aggcacgcaa
    10201 accacccttg gcgcgtatca ccgtaaatgg cagtgagaag agcaatgaaa tattccttag
    10261 ggccagtgct gtggtactgg ccaaaagaga cgctggaaga tttttatcag caagccgccg
    10321 ccagcagcgc cgacgtgatt tatcttggtg aagcggtatg cagcaagcgc cgggcaacca
    10381 aagttggcga ctgtctggag atggcaaaat cgctcgccgg aagtggcaag cagattgtgc
    10441 tctccacgct ggcgctggtg caggcatcct ctgaactggg cgaactgaaa cgctatgttg
    10501 agaacggtga gtttctgatt gaagccagcg atctcggcgt ggtgaatatg tgcgctgaac
    10561 gcaaactgcc gttcgtcgcc gggcacgcgc tgaactgcta caacgcggtg acgctgaaaa
    10621 tattgctcaa acagggcatg atgcgctggt gtatgccggt ggagctttcc cgcgactggc
    10681 tggtgaatct gcttaatcag tgcgatgagc tgggcattcg caaccagttt gaagtggaag
    10741 tcctgagcta cggtcatcta ccgctggcct actccgcccg ctgctttacc gcgcgttcgg
    10801 aagaccgccc gaaagatgag tgtgaaacct gctgcattaa gtatccgaac gggcgcaacg
    10861 tgctgtcgca ggaaaaccaa caagtgtttg tactcaatgg cattcagacc atgagcggct
    10921 acgtttacaa cctcggtaac gagctggcat ccatgcaggg attggttgat gtagtccgcc
    10981 tgtcaccgca gggcactgac actttcgcga tgctcgacgc cttccgcgct aatgaaaatg
    11041 gcgctgtgcc actgccgttg acggcaaaca gcgactgtaa cggctactgg cggcggctgg
    11101 caggactgga gctgcaagcc taagtaaata gctcactttg ttaacaactt taactattct
    11161 ttaatgcagt attaaagatt aatcggtaac aaagtgagct gttatgactg ataaaaccat
    11221 tgcgttttcg ctactcgatc tggcccccat acccgaaggc tcttcagcgc gggaagcgtt
    11281 ctcccactct ctcgatctcg cccgtctggc tgaaaagcgc ggctatcatc gctactggct
    11341 ggcagaacac cacaatatga ccggcattgc cagtgctgca acatcggtat tgattggcta
    11401 tctggcggcg aataccacca cgctgcatct ggggtctggc ggcgtgatgt tgcctaacca
    11461 ctcaccgttg gtcattgccg aacagttcgg cacactcaat acgctctatc cggggcgaat
    11521 cgatttgggg ctgggtcgtg ctccgggtag tgaccaacgg acaatgatgg cgctacgtcg
    11581 tcatatgagc ggcgatattg ataatttccc ccgcgatgtc gcggagctgg tggactggtt
    11641 tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca ggctatggcg agaaaatccc
    11701 cgtgtggttg ttaggctcca gcctttacag tgcacaactg gcggcgcagc ttggtctgcc
    11761 atttgcgttt gcctcacact tcgcgccgga tatgttgttc caggcgctgc atctttatcg
    11821 cagtaacttc aaaccgtcgg ctcggctgga aaaaccatac gcgatggtgt gcatcaatat
    11881 tatcgccgcc gacagcaacc gcgacgctga atttctgttt acctcaatgc agcaagcctt
    11941 tgtgaagctg cgccgtggcg aaaccgggca actgccgccg ccgattcaaa atatggatca
    12001 gttctggtca ccgtctgagc agtatgacgt gcagcaggcg ctgagtatgt cgttggtagg
    12061 cgataaagcg aaagtgcgtc atggtttgca gtcgatcctg cgcgaaaccg acgctgatga
    12121 gattatggtt aacgggcaga ttttcgacca ccaggcgcgc ctgcattcgt ttgagctggc
    12181 gatggatgtt aaggaagagt tgttgggata gtgtgtctta acgcgggaag ccttatccga
    12241 gctggcaacg ctgtcctacg taggcctgat aagcgaagcg catcaggcat tgtgtaggca
    12301 gcagaaatgt cggataaggc accgctgatt actgatacac cggcagtaaa ttaaagctcg
    12361 ataaaatatg caccagtgcg ttgccgacgc caaacaccag aatcagcgca atcatcggct
    12421 tgccacccca gacgcggaat ttcgggctgc caaagcgttt acgcgatgcg cgggccagca
    12481 gtgccggaac aattgccgcc cagatggtag ccgctaaacc agcataacca atggcataca
    12541 ggaatccgtt cgggaacagc agaccaccca caacaggcgg ggcaaaggtc agcaatgccg
    12601 ttttcaagcg gcccacagcc gagtcgtcga aaccaaacag atctgccaga tagtcaaaca
    12661 aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa gtttgagaac acgaccagca
    12721 gaaggtccag gctacggctg ttcagcacgc cgcttaatgc ttgtaccagc acatcaatat
    12781 taccgccctt ctcggcgatg ccgataaact ccggacgagg gatgttgccc attgtcgcca
    12841 gcaaccagat ggtatacagc gccagtgcca tcagcgtacc gtacaccagg catttcacga
    12901 tggttttcgg atctttgccg taatacttca tcaggcttgg cacgttaccg tgataaccaa
    12961 acgacgccaa gcagaacggc agggtcatca gcaaatacgg cgcataagat gcattgcttt
    13021 cggcaacgtt gaacaatgtc gcaggctgca catgccccag caggctaccg aaggtgagga
    13081 agaaggtaat gactttcgcc cccagcacaa tcgccgtcat gcggctgacg gctttagtac
    13141 tcaaccacac cacaaacgct accagcagtg caaaaccaaa acccgccgcc cgtgccggga
    13201 cgtttagcga catctctgcg aaggtgtgat gcagaatcga accactggca gaaatatagg
    13261 cataggtcag gatatagagc acaaaggcaa tggaaatgcc gttgaccacg ttccagcctt
    13321 tgcccagcaa atctttggtg atggtgtcaa aactcgaacc gattcggtaa ttcaggttag
    13381 cttccagaat catcaagccg gaatgcagca tacagaacca ggtaaagatc agcgctgcca
    13441 ttgaccagaa aaaccacgcc ccggacatga ccactggcag agaaaacatc cctgcgccaa
    13501 taatggtgcc gccgataatc accacgccgc caagcagcga cggtgacgtt tgggtggtgg
    13561 ttagtgttgc catgagggct tctctccagt gaaaaatagt gcgactgcgt tgttatgcat
    13621 tgcactgtac cagtacatga gtacaaaaga cagaaaaaaa gccccgatgg ttaaaatcgg
    13681 ggctgtatat attattttac agattgtgtt cgctgttcag cgatgattac gcatcaccac
    13741 cgaaacgacg acgaccggta gagtcatcac gacgcggtgc gcggccttca cgacgttcgc
    13801 cgctaaaacg acgaccatca ccacgaccac cttcacggcg ttcaccgctg aagttacgac
    13861 cgccttcacg acgctcgcca ccgaaaccac gaccaccgcc acgaagctca ccgccagtat
    13921 gcggctgtgc atcgcccagt aactgcatgt tcatcggctt gttgagaatg cgagtgcgcg
    13981 taaagtgttg cagcacttca cccggcatac ctttcggcag ttcgatggtg gagtgagaag
    14041 caaacagctt gatgttaccg atgtaacggc tgctgatgtc gccttcgtta gcaatcgcgc
    14101 caacgatatg acgaacttca acaccatcat cgcggcccac ttcaatgcgg tacagctgca
    14161 tatcgccaac atcacgacgt tcacgacgcg gacgatcttc acggtcgcca cgcgggccac
    14221 ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg gaattcacgt ttcggacgca
    14281 tcggcgcatc tggcggaaca atcagagtac gttcaccctg tgccattttc agcagtgccg
    14341 cagccagagt ttcgagatcc agctcttcac cttctgcagt cggctgaatt ttgctcagca
    14401 gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt
    14461 tttccagacg gcgtttgccc agcagttctg cgttcggcag ttctacttcc ggaatagtca
    14521 gcttcatagt acgttcaatg ttgcgcagca gacgacgctc gcggttctca acgaacagca
    14581 gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat acggtgaacg taagactcag
    14641 aatccatcgg gatatcgtag ttaaccacca ggctgatacg ctcaacgtcc aggccacggg
    14701 ccgcaacgtc ggtcgcaatc aggatgtcca gacgaccgtc tttaaggcgt tccagtgtct
    14761 gctcacgcag cgcctggttc atgtcaccgt tcagcgcggc gctattgtag ccgttacgct
    14821 caagagcttc agccacttcc agagtcgcgt ttttggtacg aacgaagata atcgccgcat
    14881 caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt tttacgcata ccccacacag
    14941 tccagtagct ctggctgatg tcaggacggg tagtcacgct ggactgaata cgcacttcct
    15001 gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga
    15061 acagagcggt ctgatgacct tccgggatct gcgccataat ggtttcaacg tcttcgatga
    15121 agcccatgcg cagcatttcg tcagcttcat ccagaaccag accgctcagt ttagagaggt
    15181 ccagagtgcc acgtttcagg tggtccagca gacggcccgg agtaccgaca acgatctgcg
    15241 gcccctgacg cagggcgcgt aattgcacgt cataacgctg gccgccgtac agggcaacca
    15301 catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca
    15361 gttcgcgggt cggtgccagc accagaatct gtggtgcttt cagctcagga tcaagattct
    15421 gcagcagagg taaagagaat gctgcagttt ttccgctccc cgtctgggcc atacccagaa
    15481 cgtcgcggcc attcagcaga tgtggaatac actctgcctg aattggagat ggtttttcgt
    15541 aacccagatc gttaagggct tcaaggatag gagccttcag gcccagatct gcaaaagtgg
    15601 tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa tggcggccag tctacataac
    15661 tcatcatgaa attgatcagc aattttcatt gaaaagtgtg aaccggctca aagtaggtgt
    15721 attaacgaac aacaacgccc tcacccgtta aggtgatggc aatcaaaaaa gattacgggc
    15781 tgatgtgtac gtcagctatt gctggtccga ttctgccagg tcatcttggt cctggcccag
    15841 gagcgataat tccaacaatg cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac
    15901 cgccagtttg aacagtgccg tggcgctgtc caaatccccc agacttaggt agtacttacc
    15961 taaatagaag ttggtttcac tgagatgctc agcgagcgag gtgttatcgg ttgcgtccgc
    16021 cttgagcctt tccattaacg tgtgttcgct aatgttgccc aggtagaact cgacaatgtt
    16081 ccatccccac tgttccttat ccgatttttc gaagtgctgt ttcaacactt ctttagcctg
    16141 cttctcatcg agcttctgct cggcgagata aagccacaga ctacggaaag gatcattggg
    16201 atcgtcttga taaaacgcca gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata
    16261 taatgcaatc ccgcgattca agtgcgcgta gttgtaagtt ggatcaagct caagtacaga
    16321 atcaaacgct tcataggcag catcaaaatt gcctgcctgc gttaaatata tgcctaagta
    16381 attgaatact tcaggcatat ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc
    16441 taatgccctc agaccgagac tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg
    16501 ttcgtcatcg gttaaagccc gactggcaag gatttgttcc atacgtgcca gaatcacttc
    16561 ctgctgtaaa gtcggttgca atggtaccgc gaggacttca cttttacgcc aggaagtatt
    16621 actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat
    16681 ttcccactcc cgaagaccac ggttgaatga acgtcctgtt cccggttgct aacaaggcgt
    16741 cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa tggcaacctt actcgccctg
    16801 ttcagcagcc ggagcttccg gtgctgcagc aggttgagac tgctcagtcg cttctttaat
    16861 gctcagacgg atacggccct ggcgatcaac ttccagaact ttcaccggta cttcctgacc
    16921 catctgcagg taatcggtca ctttctcaac gcgtttgtca gcgatttgag agatgtgtac
    16981 cagaccttct ttaccgccgc cgatggcaac aaatgcgcca aagtcaacga tacgggtcac
    17041 tttaccagtg tagacgcggc ccacttcgat ttctgcagtg atctcttcga tacgacgaat
    17101 agcatgtttc gctttctcgc cgtcggtcgc tgcaatcttc acagtaccgt catcttcgat
    17161 ttcgatggtg gtgccagttt cttcggtcag ggcacggata acagagccgc ctttaccgat
    17221 aacgtctttg atcttgtccg ggttgatctt gatggtatgg atacgcggtg cgaactcaga
    17281 gatatcgcca cgcggcgcgt tgatcgcctg ttccattacg cccaggatat gcagacgcgc
    17341 acctttagcc tggttcagcg caacctgcat gatctctttg gtgatacctt caattttgat
    17401 atccatctgc aatgcagaga taccctcgcg ggaacccgca actttgaagt ccatatcgcc
    17461 caggtgatct tcgtcgccca aaatgtcaga cagtacaacg tagttgtcgc cttctttcac
    17521 cagacccatt gcgatacccg caacggcagc tttgatcggc acacctgcgt ccatcagcgc
    17581 cagagacgcg ccgcacacgg aagccataga agaggaaccg ttggattcag tgatttcaga
    17641 cacaacacgt acggtgtacg ggaatttgtc catatccggc atgactgcca gcacgccgcg
    17701 cttcgccaga cgaccgtgac caatttcacg acgcttcgga gaaccgacca tgccggtttc
    17761 gcctacggag tacggaggga agttgtagtg gaacaggaag gtatcggtac gttcgcccat
    17821 cagttcatca agaacctgcg cgtcacgagc agtacccagc gttgcggtaa ccagcgcctg
    17881 cgtttcacca cgggtgaaca gcgcagaacc gtgagtacgc ggcagcacgc cagtacgcac
    17941 atccagacca cggatcatat ctttttcacg accgtcgata cgcggttcgc ctgccagtac
    18001 gcggctacga acaacgtttt tctcgatcgc gtgcagaatt tcacccagtt cgttttcgtc
    18061 cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca gatttgatga catcaacctg
    18121 cgcataacgc tcttgtttgt cggtgatgcg gtaagcatcg ctcaggcgag cttcagccag
    18181 tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc ggctgccagt cccaacgtgg
    18241 tttaccggct tctttcacca gttcattgat gttctgaata acaacctgct gttgttcatg
    18301 accgaacact actgcgccca gcatctggtc ttcgctcagc agttcagctt cagattcaac
    18361 catcagtaca gcggcttcag taccggcaac aaccagatcc agtttgctct ctttcaactc
    18421 gtcctgagtc gggttcagta cgtactggtc attgatgtaa cctacgcgag cagcaccaat
    18481 cggaccattg aacggaatac cagacagaga cagtgctgcg gaagcaccaa tcatcgcgac
    18541 gatatccggg ttaacttgcg ggttaacaga aaccacggtg gcgataacct gaacttcgtt
    18601 gacgaagcct tccgggaaca gcgggcgaat cgggcggtca atcagacgcg cgatcagggt
    18661 ttcgccttcg cttgggcggc cttcacgacg gaagaagcta cccgggatac gaccagcagc
    18721 gtaggtacgc tcctgatagt taacggtcag tgggaagaag tcctgacctg gtttggcttt
    18781 tttctggcca acaacggtaa caaataccgc ggtgtcatcc atgctaacca taacagcggc
    18841 agtagcctga cgagccatca tgccggtttc cagagtcacg gtgtgttggc cgtactggaa
    18901 tttacgaacg atcggattaa gcaatgtaat atcctttctc tttcttagac agtaccttac
    18961 ggcactggtg ttaatacccg atcttctgcg catcctcgcg actaatgaca accctaaccc
    19021 agctctatgt gggtaaagcc tctcattagc cgcgcgaacc tctgcaacgg aagatcattc
    19081 atagcaacaa tacattagtt tccagtgaat tgctgccgtc agcttgaaaa aaggggccac
    19141 tcaggcccct ttttctgaaa ctcgcaagaa ttagcgacgc agacccaggc gctcgatgag
    19201 ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg agcagtttac gacgctgaga
    19261 aaccatgcgc agcagaccac gacggctgtg gtgatctttt ttgtgctctg caaagtggcc
    19321 ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga acttcagtag aaccggtgtc
    19381 gtttgcgtca cgaccaaact cagaaacgat tttagctgtt gcttcagtac ttagagacat
    19441 tttaaaactc caaagtatat agaatgaaag gacgccgatc tctaattcag cgatcccagt
    19501 gtacgttacg caaagtgtta aacaatttac gcgacgttaa gcggcagtat tctactcgta
    19561 gcgacctgtt atcgcaagac ggttaacatt acgccgggta ttcaaccacc aggcgacgag
    19621 gcgcaacgcg gccttcatcg tcaatttcac ccataccgat aaatttgccg ttctcacctt
    19681 ctgtaacgcg aaccagtcct tccagcggcg caccggatgt acgaactggg ttaccatttt
    19741 tgaagtaaac agaagacgtt aacggaagat tcaccaccgg gtagtccgaa gctggactgt
    19801 ccattggcat cagtaatgga tcaagtaact ccgcagccgg aatatcctgc tgttcggctt
    19861 gttcgaccag ttcacgcaga tgttccaggg tcacaatccg ttcaaccgga tatttactta
    19921 ccgccagacg acgcaggtaa ataacatgcg cgccacaacc gagtttttca cccaggtcat
    19981 caatgatggt gcggatataa gtgccttttg agcagtgaat ttccagctcc agctcattgc
    20041 cttcgtggcg aataaacagc aattcataaa cggtaatcgg gcgcgcttca cgcggtactt
    20101 caatgccctg acgcgcatat tcgtacagtt ttttaccctg atatttcagt gctgaataca
    20161 tcgaagggat ctgttcgata tcgccacgga aagtatccag cgccgccgcc agttgctctg
    20221 cgctaaaggt taccggacgt tcttcaacga tttgtccgtc ggcatcagaa gtatcggtac
    20281 gctgtccaag acgcgcaatg acccgatagc gtttgtcgga gtccagcaga tactgggaaa
    20341 acttcgtcgc ttccccgagg caaattggca acatgccagt tgccagcggg tccagcgcac
    20401 cggtatgccc ggcacggttg gcgttataaa tccgtttcac tttttgcagc gcatcattgc
    20461 tggacatacc ctgcggttta tccagcaaca aaacgccgtt aatgtcgcga ccgcgacgac
    20521 gaggacgact cattagtcct ccttgctgtc gtccgggtta acacgacgtt cttcgtcatg
    20581 tttgaccacg ctggtcacca ggtttgacat gcgcatccct tcaaccagag agttgtcgta
    20641 gaagaaggtc agttccggca cgatacgcag gcgcatcgct ttccccagca ggctgcggat
    20701 gaaaccagaa gcttcttgca acgctttgat gcccgcttta accgcgtctt catctttgtc
    20761 gttgaggaac gtcacatata ctttggcata cgccaggtcg cgagacattt cgacaccgga
    20821 aacggtggtc atcatgccca ggcgaggatc tttaatttca cgctgcagga tgagagcgat
    20881 ctctttttgc atttcctgcg ctacgcgctg cgggcgacca aattctttcg ccataataaa
    20941 ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa ttaatttcag gtggaagggc
    21001 tgttcacgtt ggcctgataa gacgcgccag cgtcacatca ggcaatccat gccggatgcg
    21061 gcgtaaacgc cttatcccgc atggaaccct aaaaacctta agcgatggta cgttggatct
    21121 cgatgatttc gaatacttcg atcacatcgc cagtgcggac gtcgttgtag ttcttaacgc
    21181 cgataccaca ttccataccg ttacggactt cgttaacgtc atctttgaag cggcgcaggg
    21241 actccagctc gccttcgtag ataaccacgt tgtcacgcag aacgcggatc gggttgtgac
    21301 gtttaaccac accttcggta accatacagc ctgcgatggc accaaatttc ggcgatttga
    21361 acacgtcacg aacttccgcc agaccgataa tctgctgttt cagttccgga gacagcatac
    21421 cgctcatcgc cgctttcact tcgtcaatca ggttatagat gacggagtag taacgcagat
    21481 ccaggctttc cgcttcaatc actttacgtg cagaggcatc agcacgtacg ttgaagccaa
    21541 ccaggatggc gttggacgcc gcagccaggg tggcgtcggt ttcggtgata ccacctacgc
    21601 cagaaccgat gatcttcact ttaacttcgt cagtagacag tttcagcaag gagtcggaga
    21661 tcgcttccac agaaccctgt acgtctgcct tcaggacgat gttcacttcg tgaacttcgc
    21721 cttcggtcat gttggcgaac atgttctcga gtttagattt ctgctgacgc gccagtttaa
    21781 cttcgcggaa tttaccctga cgatacagtg caacttcacg cgctttcttc tcgtcacgta
    21841 caacggtaac ttcatcaccc gcagccggta cgccggacag gccgaggatt tccaccggaa
    21901 tggacggacc cgcttccagc acttcctgac ccagttcgtt acgcatcgca cgaacacgac
    21961 cgtattcgaa gccacacaga acgatatcgc ccttgtgcag agtaccttca cgtaccagaa
    22021 cggtagcaac cggaccacga cctttatcga ggaaggattc gataaccgca ccgctcgcca
    22081 tacctttacg taccgctttc agctccagaa cttccgcctg cagcaggata gcgtccagca
    22141 gttcatcgat accagtaccc gctttcgcag acacatgtac gaactggctt tcaccgcccc
    22201 actcttccgg caggatgccg tactgggaga gttcgttctt aacgcgatcc ggatcggctt
    22261 ctggtttatc gatcttgttc actgcaacga ccaccggaac ctgcgccgct ttcgcgtgct
    22321 ggattgcttc gatcgtctgc ggcattacgc cgtcgtcggc agcaacaacc aggactacga
    22381 tgtccgttgc ctgcgcacca cgagcacgca ttgaagtaaa cgcggcgtgc cccggggtgt
    22441 ccaggaaggt gatcatgccg ttttcagttt caacgtggta tgcaccaatg tgctgagtaa
    22501 tgccgcccgc ttcgccagag gccactttcg ttgaacgaat gtagtccagc agagaggttt
    22561 taccgtggtc aacgtgaccc atgatggtca caaccggcgc gcgcggttca gccgcagcac
    22621 ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt ttcacgacgc aggataactt
    22681 tatggcccat ctcttcagca accagctgtg cggtttcctg atcgataacc tggttgatgg
    22741 ttgccattgc gcccagtttc atcatcgctt tgatgacctg agagccttta accgccatct
    22801 tgttcgccag ttcgccaacg gtgatagttt cgccgatcac aacgtcacgg ttaacggcct
    22861 gagcaggctt ctggaagcct tgctgcagcg aagaaccttt acgttttccg cctttaccgc
    22921 cacgtactgc tgcgcgtgct tcttcacgat cagcttttga ttcagcgtgt ttgttgcctt
    22981 tcttcggacg cgctgctttc gcgttacgac cacggccacg gccgccttcg acttcacgat
    23041 cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt gacgtgataa tcgctggaat
    23101 cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc cgccatacga cgtgcttctt
    23161 cagcaacgcg acgtgcttct tcttcgagtt tacgacgcgc ttcttcttca gctttacgct
    23221 tgagctctgc agcttcctgc tcacggcggg ctttttcagc ctgggcgttt ttagtcatat
    23281 cgtcttgttg attgctcact ttgtcttttt ccgcagcttc acgtttcgct tgttcagcag
    23341 cttcacgctt agcttgttct gcggcctcac gttcagcttt ttgttgcgcc tcgcgtttag
    23401 ccgattcttc tgcctcacga cgggcttgct cttccgctgc aaggcgttca gcctcttgcg
    23461 gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc
    23521 caccggtacc aggaatgtta agggtgctgc gtgttttacg ttgcagcgtc aatttgtccg
    23581 ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcagacacag
    23641 agtcgtcagc agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg
    23701 aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg
    23761 ctacagttta ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg
    23821 ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg
    23881 cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta
    23941 ccccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg
    24001 cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct
    24061 catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg
    24121 tcgagaaacc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg
    24181 tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg
    24241 tcattacgtt gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccgttac
    24301 ggccaatcgc ctgcgccagg ttaccggctt caacggcgat atccatggtg tgtttatctt
    24361 catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcaca aactgcgccg
    24421 ggttatcatc ccacaggacg atatcgatac gctcgccgcc cagttcagta gaaaccgcct
    24481 gaacacgcgc gccacgcata cctacgcaag cacctaccgg atcgatacgt ttatcgttgg
    24541 ttttcaccgc gattttcgca cgagaacccg ggtcgcgagc cgctgcttta atttcaatca
    24601 cttcttcgcc gatttccggc acttcaatac ggaacagttc gatcagcatt tccggcttgg
    24661 aacgagtgac gaacagttgc gcgccacgcg cttccgggcg aacggaataa agcacgccac
    24721 gaacgcggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg
    24781 cttcagcatt gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc
    24841 cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt
    24901 cacgcacttt ctgcacgata acctgttttg ccgtctgggt ggtgatacgg tcaaaggtaa
    24961 cagactcaat ctgatcttca acgtaatcgc ccaagttcag gctttcatct tcataacgtg
    25021 cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac
    25081 ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac acggacgtcg atctcttgct
    25141 catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc
    25201 gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg
    25261 cgggcttttc acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat
    25321 tactcagcgc gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta
    25381 ccgctttgat aacgccctgc catttacgac ggttttgtac cgccatacgg agaaccagag
    25441 tcacctcttc tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac
    25501 ccggtgagga gacttccagg ttataagcaa cggtgatggg atcttcaaca tccagcacag
    25561 cacttacctg gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa
    25621 tatagatgcg cagtgtggat gtgcgaccgc gaataaattc gatgcctacc agttcaaaac
    25681 ccagggcctc aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc
    25741 ccacccccaa gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa
    25801 aaaccccgat aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga
    25861 gcactttcca gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat
    25921 ttgaaaaagg actctaaggg aaagtggttg cgggggccgg atttgaaccg acgaccttcg
    25981 ggttatgagc ccgacgagct accagactgc tccaccccgc gcctgaaacg tggcaaattc
    26041 tactcgtttt gggtaaaaaa tgcaaatact gctgggattt ggtgtaccga gacgggacgt
    26101 aaaatctgca ggcattatag tgatccatgc cacattttgt caacgtttat tgctaatcac
    26161 gtgaatgaat atccagttca ctttcatttg ttgaatactt ttgccttccc ctgctctccc
    26221 ttaagcgcat tattttacaa aaaacacact aaactcttcc tgtctccgat aaaagatgat
    26281 taaatgaaaa ctcatttatt ttgcataaaa attcagtgag agcggaaatc caggctcatc
    26341 atcagttaat taagcagggt gttattttat gacgacgatt ctcaagcatc tcccggtagg
    26401 tcaacgtatt ggtatcgctt tttccggcgg tctggacacc agtgccgcac tgctgtggat
    26461 gcgacaaaag ggagcggttc cttatgcata tactgcaaac ctaggccagc cagacgaaga
    26521 ggattatgat gcgatccctc gtcgtgccat ggaatacggc gcggagaacg cacgtctgat
    26581 cgactgccgc aaacaactgg tggccgaagg tattgccgct attcagtgtg gcgcatttca
    26641 taacaccacc ggcggcctga cctatttcaa cacgacgccg ctgggccgcg ccgtgactgg
    26701 taccatgctg gttgctgcga tgaaagaaga tggcgtgaat atctggggtg acggtagcac
    26761 ctataaagga aacgatatcg aacgtttcta tcgttatggt ctgctgacca atgctgaact
    26821 gcagatttac aaaccgtggc ttgatactga ctttattgat gaactgggcg gccgtcatga
    26881 gatgtctgaa tttatgattg cctgcggttt cgactacaaa atgtctgtcg aaaaagccta
    26941 ctccacagac tccaacatgc ttggtgcaac gcatgaagcg aaggatctgg aatacctcaa
    27001 ctccagcgtc aaaatcgtca acccgattat gggcgtgaaa ttctgggatg agagcgtgaa
    27061 gatcccggca gaagaagtca cagtacgctt tgagcaaggt catccggttg cgctgaacgg
    27121 taaaaccttt agcgacgacg tagaaatgat gctggaagct aaccgcatcg gcggtcgtca
    27181 cggcctgggc atgagcgacc agattgagaa ccgtatcatc gaagcgaaaa gccgtggtat
    27241 ttacgaagct ccggggatgg cactgctgca cattgcgtat gaacgtctgt tgaccggtat
    27301 tcacaacgaa gacaccattg agcagtatca cgcgcatggt cgtcagttgg gccgtctgct
    27361 gtaccagggg cgttggtttg actcccaggc gctgatgctg cgtgactctc tgcaacgctg
    27421 ggttgccagc cagatcactg gtgaagttac cctggagctg cgccgtggga acgattattc
    27481 aatcctgaat accgtctcag agaacctgac ctacaagcca gagcgtctga cgatggaaaa
    27541 aggcgactcg gtgttctcgc cagatgatcg tattggtcaa ttgaccatgc gtaatctgga
    27601 tatcactgat acccgagaga aactttttgg ttatgccaaa actggcctgc tttcctcctc
    27661 tgccacttca ggcctgccgc aggtggagaa tatggaaaac aaaggccagt aattcgacat
    27721 caaccctgcc cttcaggggt ggggttacct tgcttatcct ctgtaaactc ctttctgagc
    27781 atcacataat cgaaaacatc atggtttgca tccacaaccc gtgagtctcc tttatagtgt
    27841 gcaatcactt cctcaagcgt ttgcggctgt tccggctttg ttacccccat ccacgcatta
    27901 ataaggtaat tattgtaagc tgtcgaaaag atattgtttt ccgttgtgcg atccacgcca
    27961 tccccaagaa cggggctata acagataaac atcgggacat gatatgcctg ctggctagcc
    28021 tccctgcctc catgaaaata gacgttcttc ttcgtagggt cacgttccag gccgtggtcg
    28081 gcaaaataca tgaccgaggc gcggcgatct tttaataatt caaaaaactg acctagcaaa
    28141 ctatctgtgt aatgaatgga gttgtcatag caggcatcct gatcgtcctg cggttgaaac
    28201 acgcgctgga ttgcggatag gcgctacaag ccggttcatg gcttccattt aaatgaagaa
    28261 caatcagttt cttttgctgc gtattttgct gtaatgcttg cgataaatgc ggcaacaaca
    28321 attcatcaaa tcctctgaca taaactgttt ccatggcgcg catggcgata ctggtaactg
    28381 ctgtaccatt ctgccgaaaa gcggattgcg agcttagcca gaaagtctga aatcctgctt
    28441 gattagccat attaataatg ttgtcggggt aattatgaat gtcatgactc aaaacagaat
    28501 cagcagttaa agaaagggga accgacagcg cagtgtaagg tgcgccgctt attgcttgat
    28561 taaacagttt gatctgtttt ctttgtgctt caacttgcgg tgtcgtagag cgtgtatatc
    28621 catacaaaga catattgtcg acacgtacag actcccccac aatcaacacg taggtatcaa
    28681 tacttgtatc cctgactgat aattgaaaat acggcaccgt gtttgcaatt gagagtaatc
    28741 tttgatgctc tttcgctgct aaagcaaaat agttgagatt gaaaaacggc gtataggtag
    28801 caaatcacga cgctagtata tatggactga acgctttttt attttttgca tctttataag
    28861 caaattgaca agcggaaaat aaactgcccg aaatgacaat cagcaataat attcctgtca
    28921 ctttttttgt cggcaaggaa acatcatatt ttattattac tgccaaaaaa aaaagggata
    28981 aaaaagcaaa agcacataga taagggatat acatccccag cattttcaca acttcatccg
    29041 gatcactctg cagcacacta atcgcaaatc catcattgaa agttgtacca aaggtacacc
    29101 atgaatagag gctaatactc atgtctgccg cagtcagtaa cacaaatggt gcggcaacaa
    29161 gcaggcgaag cggaagggat gatatacgtt taacaaccag gatggtcagg acgaagaaca
    29221 gcaatcgctg aaccatatgc gctccggaag aggcgactaa gttcgtaata ccaaaaacaa
    29281 taacacaaag atacaacaac caatgagatt taaaatcttc tagcaaattt gttgaatact
    29341 gtcatccaga aatccaagtt cacaaccaat ataaacaatt atcaacaatg ttcaatttta
    29401 tatcagacat gtttttcaac tctgaaccat atcagaccga aacactgtta gttttttctt
    29461 tttaaatgat gcgaccattg cgttctcagt aattaaataa cattatcaat gcgtctctaa
    29521 acattacgca gaacgtagcc atcagtagtt atactgacag gagcaggatg gtttatcgag
    29581 aagtaaacat atccgtattc gtaccggaac tggagttgac acaataaagt gccaattatg
    29641 tcagcagtag ggaaaagttt ataactaagg cgtaccggca ccatcgtttc aaggtaccag
    29701 ctacgagtaa agcaactgga cgagatacag atacctgaca accattcatc agaccaggac
    29761 caaaacgaaa agagacgctt ttcagcgtct cttttctgga atttggtacc gaggacggga
    29821 cttgaacccg taagccctat tgggcactac cacctcaagg tagcgtgtct accaattcca
    29881 ccacctcggc acggatacta ctttttagtt cgggatatcg ctggtcggct tagccggagc
    29941 agctggctga gtttgttcgg ttttcgccgg tgcactcaga ttttcccatt cgctacctct
    30001 attggttttg ttgctattga tgttacccag caccagactg atgatgaaga ataacgttgc
    30061 cagcagcgcc gtcatgcggg tcatgaagtt accagaacca cttgaaccaa acagcgtagc
    30121 ggaagcgcct gctccgaagg aggctcccat atcagcgcct ttaccttgct gcagcatgat
    30181 cagaccaaca aggccaattg ccacaataag gaaaactact aaaagagctt cgtacataat
    30241 caacctgttc cttgcggagt tgccgcgtac caatgcttca accaataaag cgggagtttt
    30301 taatctttcc cactgaagcg ggtgtgaata ctaaccaaag cgaatgacct tcgcaagggc
    30361 aattttatcg cattgtatca actgcggaaa aaaacagcaa aaccgatgtg ttcgccgaga
    30421 aaaaaggcga caatcgccgc ctttttagcc agttatctaa cgctttaaac ggcttttact
    30481 gcatcggcga tgcggtgtgc aaattcagtc acctgcgctt cgtcttcgcc ttccaccatc
    30541 acgcgaatta acggttcggt gccggattta cgcagcaaca cgcgtccacg gttgcccagc
    30601 gcagcttcaa cctctgcggt cacggcttta actgactcat gctcaagcgg atcgccgcta
    30661 cctgcggtgt aacgtacgtt aaccagaatc tgcgggaaca ttttcatgcc gctgcaaagg
    30721 tcgtgcaggc tcatatggtt acgtgccatc gccgccagca cctgcaagcc agcaacgatg
    30781 ccgtcaccgg tagtggtttt atccagcagg atcacatgac cggaattctc tgcaccgata
    30841 cgccagcctt tctcctgcat tttttccagt acgtagcggt cacccacttt cgcgcgcgca
    30901 aatggaattc ctaactgttt cagcgccagt tcaagcccca tgttgctcat caatgtaccc
    30961 acagcgccac cacgcagctg gccctgacga agaccttcac gcgcgatgat atacatgatc
    31021 tgatcgccat cgactttatt gccttcatgg tcaaccataa tcacgcgatc gccgtcgccg
    31081 tcgaaggcaa taccgagatc cgctttttca gccagcacac gagcctggag cacgcgaacg
    31141 tcggtagccc ccacttcggc attgatgttt acaccgtttg gctcacaacc gatagcgata
    31201 acgttcgccc ccagttcgcg cagcacgttc ggcgcgatgt gataagtcgc accgtttgca
    31261 caatccacca caatcttcag ttcactgagg ctaagttcgt tcgggaacgt ggctttgcaa
    31321 aactcgatat agcgacccgc ggcatcaacg atacggctgg ctttacccag ttctgccgaa
    31381 tcaacgcagc tgatctcctt ttccatttcc gcttcgatgg cctcttctac cgcatccggc
    31441 agtttagtgc cgtcgataga gaagaattta atcccattat cgtagaacgg gttatgcgat
    31501 gcagatatca caattccggc ctctgcgcgg aaggtacgcg tcagataagc cacggccggt
    31561 gttggcatcg ggccagtgaa gagtgcggaa aggcccgctg ccgccagacc cgcttccagt
    31621 gctgactcca gcatatagcc agaaatacgc gtgtctttac caataataat cttacgggag
    31681 ccgtggcgcg ccagcacttt acccgcggcc caacccagct taagcacaaa atcaggtgtg
    31741 atcggcgcat cccctacacg accacgaatc ccatcggtac cgaaatattt acgattactc
    31801 atagcgtttg ttttcctttg cagacagagt ggcttccacc acccgcatcg cttctacggt
    31861 ttctttgacg tcatgaacac gaatgatgtg cgcgccttgc attgcggcaa tgaccgcaca
    31921 ggccagacta ccgctcaggc gctcggacgg ccccacgttc agcagctgcc caatcatcga
    31981 ttttcgtgac atacccacca acagcggcag gttgaaatgg tgaaattcag ccaggcgcgc
    32041 cagtaatgaa tagttatggg agagattttt accgaaaccg aatccggggt cgagcaacaa
    32101 tttctctttt gcgatacccg cctgctcgca acgtgctatt tgctcaataa agtagcgatt
    32161 cacttctgca aagacatcgt catacttcgg agcttcctgc atggtttttg gatttccctg
    32221 catatgcatc agacaaaccg gtaaaccggt ttctgcagcc gcctccagag cgccaggttc
    32281 ggaaagggag cggatatcat taataatgtg agcgccaact ttcgctgact cacggatgac
    32341 ttctggtttg gatgtatcga ccgagatcca gacttcgaag cgttgagcaa ttgcctcaac
    32401 cacaggaata acacgttgca actcttcttc aacgctaact tccgccgccc ctgggcgcgt
    32461 ggactcgcca ccaacgtcaa tgatcgtcgc gccagcattg atcatcagat tcgcatgttt
    32521 caccgcatct atcagcgagt tatgcgtgcc accatccgaa aaggaatcag gcgtgacgtt
    32581 gaggatcccc attacgtgag gatggctaag gtccagtgaa gtaccctggg caaagagttt
    32641 catgatgtta tccctggtat gaattgataa gaaaaaaccc cggagcacgc cccggggttt
    32701 tcggtacaaa tacagtcatc tgatgcggga acttacttgt cgcctaactg ctctgacatg
    32761 gtgttacccg ggttcggcgt acgcggttca tcaaccggac gaggagcctt tggactacca
    32821 ttgtcgccag aattgttaga agcgcctggt tcttcccagc ccgctggcgg acgtacatcg
    32881 cgacgtgcca tcaggtcatc aatctgcggt gcgtcgatag tctcatattt catgagagca
    32941 tctttcatcg catgcagaat atccatattg tcggtcagaa gctgacgcgc acgattatag
    33001 ttacgctcaa tcagtgcttt cacttcctgg tcgatgatac gtgcagtttc atcggacata
    33061 tgtttcgctt tcgctacgct acggccgagg aatacttcac cttcttcttc cgcgtacagc
    33121 agtggaccca atttctcaga gaagccccac tgagtcacca tgttacgtgc caggttggtc
    33181 gcaactttaa tatcgttgga cgcaccggta gaaacatgtt ccggcccgta gatgatctct
    33241 tctgccagac gaccaccgta cagcgtagaa atctggcttt ccagtttctg acggctggcg
    33301 ctgattgcgt cgccctcagg caagaagaaa gtcacaccca gcgcacgacc gcgtgggata
    33361 atcgtcactt tatgcaccgg atcgtgttcc ggcaccaggc gaccgataat cgcatgaccc
    33421 gcttcgtggt aagccgttga ttctttctgc gcttccgtca tcaccatgga gcgacgttcc
    33481 gcacccatca tgattttgtc tttcgctttc tcgaactcaa ccatcgacac aacgcgtttg
    33541 ttgccacgag cagcgaacag tgccgcttcg ttcaccaggt tcgccaggtc agcaccggag
    33601 aaaccaggag taccacgggc aatgattgcc gcgtcgatat cgggtgccaa tggtacgcga
    33661 cgcatgtgaa ctttcaggat ctgctcacga ccgcgaacat ctggcaagcc gaccacaacc
    33721 tggcggtcga aacggccagg acgcaacagg gccgggtcga gaacgtccgg acggttagtc
    33781 gcggcgataa cgatgatacc ttcgttacct tcgaagccat ccatctcaac cagcatctgg
    33841 ttcagagtct gttcacgttc atcgtgacca ccgcccagac cagcgccacg ctggcggcct
    33901 acggcgtcga tttcatcgat aaagatgatg cacggtgccg ctttcttcgc ctgttcgaac
    33961 atgtcacgaa cacgggatgc acccacaccg acgaacattt ctacgaagtc agaaccggag
    34021 atagtaaaga acggaacttt cgcttcgcct gcaatcgctt tcgccagcaa cgttttaccg
    34081 gtacccggag gaccgaccat caggacgcct ttcgggatct taccgccgag tttctggaag
    34141 cggctcggct cgcgcagata ctctaccagt tcagcaactt cttcttttgc ttcgtcgcag
    34201 cccgcaacgt cagcaaaggt cgttttgatc tgatcttccg tcagcatgcg cgctttgctc
    34261 ttaccaaacg acatggcacc tttgccaccg ccgccctgca tttgacgcat gaagaagatc
    34321 cagacaccaa tcagcaacag catcgggaac caggagatga agatagaagc cagcaggctt
    34381 ggttcttcag gcggttcacc gacaaccttg acgttcttgg tcaacaggtt atccagtaat
    34441 ttcggatcct gaaccggaat gtaagtggta taacggttac tatctttctt ggtaacgttg
    34501 atttcacgtc cgttgatacg cgcttcacga acctggtcgt tattgacctc ttgtaggaag
    34561 gtagagtaat ccaccttacg gccattagac tcgctgggcc caaagctctg gaatactgac
    34621 atcagcacaa cggcaatgac cagccagagt attaggtttt tcgccatgtc actcaaggga
    34681 ttaacctctt attacaactg tgttaaaaac agcgtcagga tactctatat ccagcatctt
    34741 tcaaactttc gtctgaaatc tcccggttag ggtttacgcc cggtcgctac aatatacact
    34801 tctcgcgaac gtgcacgaga agagtccggc ttacgaactt tgaccttcgt aaacagggag
    34861 cgaatttccc ttagatactc atcgaaacct tcgccctgga acaccttcac tacaaaactg
    34921 ccacctggcg ctaatacatc acgacacatt tctagcgcca gttccaccag atacatggca
    34981 cgggggatat ccaccgccgg tgttccgctc atgtttggtg ccatatcgga catgacaacc
    35041 tggactttgc tgtcgccaac gcgctccagc agtgctttca tcaccagttc atcacgaaaa
    35101 tcgccctgaa gaaagtccac accaacgata ggatccatag gtaaaagatc gcaagcgatg
    35161 atgcggcctt tgccgccaat ttgggtgacc acatattgtg accaaccacc cggagcagca
    35221 ccaaggtcga caaccgtcat tcccggttta aagagtttgt cactttgctg tatttcatca
    35281 agtttaaacc aggcacggga acgtaatccc tttttctgtg cctgttgaac atatttatcg
    35341 ctaaagtgtt cctgaagcca gcggctggag ctggcagaac gcttcttacc tgtcatttaa
    35401 ctttcccatg gggataactc atcgtaacca attgcgtaaa tttttacgcg cctatttggt
    35461 gatatatggg agatggcggt agaatgaccc gttttcaatc ccaacgtaag caaaaaatac
    35521 gatgaatctg agtactaaac aaaaacagca cctgaaaggt ctggcacatc cgctcaagcc
    35581 agttgttctg cttggcagta atggtttgac cgaaggggtg ctggccgaga ttgaacaagc
    35641 gttagagcac catgaactca tcaaggtgaa aatcgccacc gaagatcgcg aaacaaaaac
    35701 cttgatcgtg gaagctatcg tgcgcgaaac cggcgcctgt aatgtacagg tcatcggtaa
    35761 aacgctggtg ctttatcgcc caactaaaaa acgtaaaatc tcgctgccac gctaagatta
    35821 tcctaaagtt acacacattt gctgtgtaaa acgaggggtt ttccgcaggc aggagagcaa
    35881 aatgccacgc tctgttcgtt gataaaaggc cgcagagcgg cctttttcct ttctttacaa
    35941 tacatcaaca tcttgagtat tgggtaattc ttacaggtat tccaccttaa ttacttcaaa
    36001 ttctacttcg ccgcccggcg ttttgatgac cacaacatca tcttcttctt tgccgatcag
    36061 gccacgagca ataggcgagt ttacagaaat caggttttgt ttaaagtcag cttcgtcatc
    36121 gccaacgatg cgataagtct gttcttcgtc agaatccaga ttcagcaccg ttacggtagc
    36181 accaaaaata acgcgcccat tgttgggcat tttggtgaca tcaatcacct gcgcgtttga
    36241 cagcttggct tcgatgtctt tgatacggcc ttcgcagaag ccctgctgtt cacgagctgc
    36301 gtggtattcg gcgttttctt tcaggtcgcc atgctcacgc gcttccgcga tagcagcaat
    36361 gatttcagga cggcgcacag atttcagaaa atccagctct tcgcgtaatt tttcagcgcc
    36421 gcgtaaggtc atcggaatag cttgcatttg ttatacctct tgaatattcc tgatggggca
    36481 agtcttcacc caccccggct gttcggcctg cccggcataa ctcctcaccg ggaccagaag
    36541 caaaaaaata ccgacccggg tacaagtccc aggtcagcta caattcacat tttgatggtc
    36601 attttaccct gaagttcccg aagggtcatc gtttacttta tagggcgttg cgccgtagta
    36661 tgacggctcg attccaggtt gttagcgcga gattatgcga ttttccagat ttatcatcgg
    36721 attgaccagc tgtatagcgt tcagtgttca ggccgcgaat gttgatgagt acgttactca
    36781 actccccgca ggtgccaacc ttgccctgat ggtgcaaaaa gtcggtgcgt cggcccccgc
    36841 tattgattac cacagtcagc agatggcgct gcccgccagt acccagaaag tgattactgc
    36901 gctggcggcg ttgattcaac tcggccccga ttttcgtttc accacgacgc ttgaaaccaa
    36961 aggcaatgtg gaaaacggcg tacttaaggg tgacttagtg gcgcgatttg gtgccgatcc
    37021 gacgttaaaa cgtcaggata ttcgcaatat ggtcgcgact ttgaaaaaat ctggcgtcaa
    37081 ccaaatcgat ggcaatgtgt tgatagatac ctccattttc gccagccacg ataaagcccc
    37141 cggctggcca tggaatgaca tgacacaatg ctttagcgct ccgcctgctg ccgccatagt
    37201 tgaccgcaac tgtttctccg tctcgctcta cagtgcccca aagcctggtg atatggcttt
    37261 tatacgcgtg gcatcttatt accccgttac gatgttcagc caggtacgca ccctcccccg
    37321 tggttctgcc gaagcgcaat actgcgaact ggatgtggtg ccaggcgacc tgaaccgctt
    37381 tacgctgacg ggatgcctgc cacaacgttc tgagccgctc ccgttggctt ttggcgtgca
    37441 ggatggagcc agctatgccg gtgcaattct gaaagatgag ttaaaacagg cgggtatcac
    37501 ctggagcgga acactgctgc gccagactca ggttaacgaa cctggaacgg tagttgccag
    37561 taaacagtcg gccccgctgc acgatctgct taagattatg ctgaaaaagt cggacaacat
    37621 gatcgccgat acggttttcc gcatgatcgg ccatgcgcgc ttcaatgtgc ctggaacatg
    37681 gcgggctggg tcggacgccg tgcgtcagat cctgcgccag caagccggtg tcgatattgg
    37741 taacaccatt attgccgatg gttcagggct ttcgcggcat aacctgattg cccccgccac
    37801 catgatgcag gtgctgcaat acattgccca acacgacaat gaacttaact ttatctccat
    37861 gctgccgctt gcgggctatg acggctcttt gcagtaccgt gcaggtctgc atcaggcggg
    37921 cgtggatggc aaagtctcag cgaaaaccgg ttcgttgcag ggggtatata acctggcggg
    37981 attcattacc acagcgagcg ggcaacgaat ggcatttgta caatatcttt ctggctatgc
    38041 agtaaaaccc gcggatcagc gtaatcgccg tattccgtta gtgcgttttg aaagccgttt
    38101 gtacaaagat atttatcaga acaattagtc aaaaagaaac cccggcgcat ggccggggtt
    38161 tcagattatt gacaaagtgc gctttgttta tgccggatgc ggcgtgaacg ccttatccgg
    38221 cctacaaaat cgtgcaaatt caatatattg caatcatctt gtaggcctga taagtgtagc
    38281 gcatcaggct tatttggcat tggtcattag tgattaacgc ttgtaaatga actcaacgcc
    38341 ttcttcgtcg tcttcgtccc agtcgtcatc ccagtcttca tcatcctctt cagcaatctc
    38401 ttcaagctgc tgacggtgat aatcatccca catgaattcg actttctctg gctgtttcgc
    38461 ttcttcagcc tgcacgaccg ggttttcaat gataaaggtc atcacatccc agcagagatc
    38521 tttcacgccc agtccgctcg ccgcagagat cagataatat ttatcttccc agcccagcgc
    38581 ttcagcgatc gctttcgctt tctcttcggc ttctgcctta tccagcagat cgatcttgtt
    38641 gaagactaac caacgcggtt tcgccgccag atcctggctg tatttttcca gctcgctgat
    38701 aataatacgc gcgttttcaa ccggatcggt gccgtcaatc ggatcgatat cgatgaggtg
    38761 caacaggacg cggcaacgtt ccaggtgctt caggaagcga atgcccagac ctgcgccttc
    38821 cgcagcgcct tcaatcagtc ctggaatatc ggcaacaacg aagctctttt cgttgtccat
    38881 tcgtaccaca cccagacttg gcaccagagt ggtaaacgga taatccgcca ctttcggttt
    38941 agccgccgat accgcacgaa taaaggtcga tttacccgcg tttggcatcc ccaacatacc
    39001 gacgtcagcc agcagcatca gctccagcag cagctcacgc ttatcgcccg gcgtaccgtt
    39061 agttttctgc cgtggtgtac ggttaactga cgatttgaaa cgggtattgc ccagaccgtg
    39121 ccagccgccc ttagcaacca gcagacgctg accgtgttta gtcatatcac ccatggtttc
    39181 accagtaccc tggtcgatta cacgcgtacc taccggcact ttaatcgtca cgtctttacc
    39241 gcgcttaccg gtacaatcgc ggcttgcgcc gttctgaccg cgctctgcac ggaaagattt
    39301 ttcaaaacga taatcgataa gcgtgttcag gttctcgtcg gcttccatcc atacgtcacc
    39361 accatcaccg ccgtcgccgc catccgggcc gcctttcgga atatactttt cgcggcggaa
    39421 gctcacgcaa ccattaccgc catcacctgc aacgaccaga atcgatgctt catcaacaaa
    39481 cttcatttta ttctccgtaa atcattcgcc tgcgcggggt tgcgatacca ccgttgtatg
    39541 cttacgtaat ccgccccaaa tacgatgacc aatggcggaa tacatcgcgc ccgcaaccac
    39601 gacaaacgca ccgagataac ctaaaaggtt taacatcggt ctggcgaaga aatcgggcca
    39661 ggccagtgat aaaagatctg aaaaaaacag cgtaaacagt ggggtgagcg tgatgatcgc
    39721 gctcacctga gctgcctgcc agcgagccat cgcttccgcc aaggcgccat atcctaccaa
    39781 ggtattcagt ccgcaaaaaa ttaaacatgc gagctgccag tggctaagct gcgctatcac
    39841 tccaggcttt gccagaggga agagcgcgat tgtacataaa gtgtacagta aaaacaggat
    39901 ctgcggtgag gccagccgac gcaataaaac cttttgcgcc acgccataac tcacccaaac
    39961 cgtcgccgca ccgaccccaa agataactcc ccaggtgtaa tcggtgagct ttgtaaatat
    40021 ctcgaccaga ctggtgttaa aaaacatcac cagaccgctc aggagcatca atgcccctac
    40081 aacctgagtg ctgcgcattt tctctttcag gataaatacg ctggcaacca tcatgccaac
    40141 tggcgagagt tgcccaatca cctgcgaagc ggtcggactc aggtattgca aggatgagct
    40201 gaacaggatg aagttcccaa acagcccggc ggtcgccact gccaacaaaa tcaaccagcg
    40261 tggcttacga aacacgcgta atggcggcaa cctcttcttc accgcaagaa tggcaccaag
    40321 gccaatactc gccatcaaga aacggtaaaa cacgattgtc ggaggttcca tcacctccag
    40381 cacctgcttc attgcgattg gcaacgcccc ccagcaaatt gctgtggtaa gcgccaaaag
    40441 aatgccaatg cctgcctgct gcttcatacc gtattccctg caaagcgcat ttgcggatta
    40501 acgtcgcacc atgcgcattt ttaccggtta tcgaatgtaa aaagccccgc aacgtgttgc
    40561 ggggctttca tccgttaccg ggacgcgaaa aacttattca gcttcgatgc tgataaattt
    40621 acggtttttc gggcctttaa cttcgaattt cactttaccg tctgctttag caaacagagt
    40681 gtggtcacga ccgcaaccta cgttagcgcc agcgtggaat ttggtaccac gttgacgaac
    40741 gatgatgcta cccgccagaa cggattcgcc accgaaacgc ttaacgccca ggcgtttagc
    40801 ttctgaatcg cgaccgttac gtgtggagcc gccagccttt ttatgtgcca tttgaaatct
    40861 ctcctcaggt cttaggcgct gatgccagta attttcacat cagtgaacca ctgacgatgg
    40921 ccctgctgct tacgatagtg tttacgacga cgaaacttaa cgattttaac tttctcgcca
    40981 cgaccgtgag caacaacttc agctttgatt acgccgccat caacgaaagg aacgccgatt
    41041 ttgacttctt caccgtttgc gatcatcagc acttcagcga actcaacagt ttcgccagtt
    41101 gcgatgtcca gcttttccag gcgaacggtc tgaccttcgc ttactcggtg ttgtttacca
    41161 ccactttgga aaaccgcgta catataaaac tccgcttccg cgcacacctt ttcaatgatt
    41221 caaagtgcgc tataaatatt cacaataggg cgcgaatatt acgcaaaacg cacgcctttg
    41281 acaagtgcta cagtcaatac acgaagaaaa aaaacacaac ttgtacggta acgtttatct
    41341 gtgccatttt ttcagtacaa ttaccctata ttcctaacca taaaccctaa gttgcctttg
    41401 ttcacagtaa ggtaatcggg gcgaaaagcc cggcttttgc gatgaattta gaaaaaatca
    41461 atgagttaac cgcgcaagat atggcgggtg ttaatgcggc aatccttgag cagcttaatt
    41521 ccgacgtcca actgatcaat cagttaggct attacatcgt cagcggcggc ggtaaacgta
    41581 ttcgtccgat gattgctgta ctggctgcac gagctgttgg ctatgaggga aatgcgcatg
    41641 tcaccattgc tgccctgatc gagtttatcc acacggcgac tctgctacac gacgacgttg
    41701 tggatgaatc agatatgcgc aggggtaaag ctaccgccaa cgccgcattt ggcaatgccg
    41761 ccagcgtgct ggtaggcgat tttatttata cccgcgcgtt ccagatgatg accagcctcg
    41821 gttcactcaa agtgctggaa gtcatgtcag aagctgtaaa cgtcatcgca gaaggtgaag
    41881 ttctgcaact gatgaacgtt aacgatccgg acatcactga agaaaactac atgcgcgtta
    41941 tctatagcaa aaccgcgcgt ctgtttgagg ctgccgcgca gtgttccggg attctggctg
    42001 gctgtacgcc ggaggaggag aaaggcctgc aggattatgg gcgctatctc ggcactgctt
    42061 tccagttgat cgacgattta ctcgattaca atgccgatgg cgaacagtta ggtaaaaacg
    42121 tcggcgacga tctgaacgaa ggtaaaccga cgctgccgct gctgcatgcg atgcatcatg
    42181 gcacaccaga acaggcacag atgatccgta ccgccatcga acaaggtaac ggtcgccatc
    42241 ttctggaacc ggttctggaa gcaatgaacg cttgtggatc tcttgaatgg acgcgtcagc
    42301 gcgccgagga agaagcagac aaagccatcg cagcgttaca ggtgctcccg gacacccctt
    42361 ggcgagaagc actcatcggc ctcgcgcaca tcgctgttca acgcgatcgt taatcccctc
    42421 ccttcatccc gcgcagtgcg cgggatgatt ccagtaaatt ccataaaaac actaattcag
    42481 cgctataaca cctgaaaaaa agggcgatga atctttatat attctgaata ttcacactct
    42541 ttacaggaac tttttagcgc aataggccat caggagtata gtgatgcccg acagaagaag
    42601 tgttctgaat gaaagcgact aacttaagga gtgaggaaag atggaaagta atttcattga
    42661 ctggcatccc gctgacatca ttgcaggttt gcgcaagaaa ggaacgtcaa tggcggcgga
    42721 atctcgcaga aatggtttga gttcctcaac gctggcaaac gcattatcgc gcccatggcc
    42781 gaaaggagag atgattattg cgaaagccct gggaactgac ccctgggtta tctggccatc
    42841 acgctaccat gatccgcaga cccatgagtt tatcgacaga acgcagttga tgcgtagcta
    42901 cactaaaccg aaaaaatgag tggtctggcg gtagccccgc gaacggggct gccagctctc
    42961 agacgattac tcgcctttca cacgctcaat atttgcacct aaagcgcgca gtttgtcttc
    43021 aatgcgttcg tagccacgat cgatgtgata aatacgatca accaccgtcg tcccttccgc
    43081 aatacagcca gccagcacca ggcttgctga tgcacgcaga tcggttgcca taacctgtgc
    43141 accagaaagt ttttcaacac catgacaaat aacggtattg ctttcgattt cggcgtgcgc
    43201 gcccatacgg ctcagctctg gcacatgcat aaagcggttt tcaaagaccg tttcggtgat
    43261 gaacccggtc ccttcagcca ccaggttcaa cagcgtgaac tgggcctgca tatcggtcgg
    43321 gaatgccgga tgcggcgcgg tacgtacgtt aacagccttc ggacgtttgc catgcatatc
    43381 caggctaatc cagtcttcgc cgacttcgat gtccgctcca gcatcacgca gtttcgccag
    43441 cacggcgtcc agagtatctg gctgcgcgtt acggcagata attttgccac gagagatcgc
    43501 cgccgccacc aggaaagtac cggtttcgat acgatccggc agcacgcgat agacaccgcc
    43561 gcctaaacgt tccacacctt cgatgacgat acgatcggtg ccctgaccgc taattttcgc
    43621 acccagcgta atcaggaagt tcgcggtatc gacgatttcc ggttcacgcg ctgcgttttc
    43681 aataatcgtg gtgccttccg ccagggttgc agcacacatg atggtcaccg ttgcgccaac
    43741 gctgacttta tccatcacga tatgtgcgcc tttcagacga ccatcgacgg aagctttaac
    43801 gtaaccttct tccagtttga tggtcgcgcc taattgttcg aggccagaaa tgtgtagatc
    43861 aaccggacga gcaccgatcg tacaaccgcc aggtagtgaa acttgcccct gaccaaagcg
    43921 cgctaccagc ggccccagcg cccagataga agcacgcatg gttttaacca gatcgtaagg
    43981 tgcgcagaat acattaacgt cgcgggcatc aatatgcaca gaaccattac gttctacttt
    44041 cgcacccagc tggcttagca gtttcattga tgtatcgacg tctttcagtt tcgggacgtt
    44101 ctggatctct accggttctt ccgccagtag tgcggcaaaa agaataggca gagcagcatt
    44161 tttagcgccg gaaattgtga cttcgccctg gagcttcgtt ggcccctgaa cacgaaattt
    44221 atccatttag tttgttctca gttaacaatt catatccgct accggcgaat cgcccatagc
    44281 tcaaaagccg ttcagtttgc gatcgcgcgc ccactccgca ggggtatacg ctttgatcga
    44341 cacagcatga atgcggttat ccgcaatata ttccatcagc gggccataga ccgtctgctg
    44401 ttttttaacc cgactcatac cgtcaaacaa ctcacccacg gcaataacct gaaagtgact
    44461 gccatcgccg gaaacgtgga cttcctggag ggagagagcg ttcatcaaca cgctctgaat
    44521 ttcattattt tccatgggat cttcaatcat cagttaataa accagcgaaa catcttagag
    44581 caaagttgcg ctggcataaa taagcaaaaa gcctcgctga taaatcagac aaggctcgac
    44641 ttgcaggcag gtttgccgga caggcggtta acgccatatc cggcctgaaa aaatttaacg
    44701 aggcagaaca tcagcaggca aattatacaa tttcgcgagg gtatacactt tgtcgtttac
    44761 cccctgaagc gtcacgttgt tgccctgctt tttcgccaga tcgataagat ggagcagcag
    44821 tgccagtccc cccgtatcca cgcgggacac acggctaaga tcgatgcagg taatcccctt
    44881 caccgcttcc tcacgcattt cccaaagcgg tagcagaacg tcctgatcca gctctccgga
    44941 taacgccagc gtgtcacccg tctgcatcca gctcagtgac tcgctcatta ttttttctct
    45001 tccagagtga ttttctgttg agaaatcgat ttcagttgcg cagtcaggcc gtcgatacct
    45061 ttggtacgca gcagcgttcc ccactcgttt tgtttggtgg tgatcatact gacgccttca
    45121 gcaatcatgt cgtaagcttg ccagtttccc gtctgggagt ttttacgcca ctggaagtcc
    45181 agacgcaccg gcggacggcc attcgggtca ataatggtaa cgcgaatagg cacaatggtt
    45241 ttatcgccca gcggctgttc tggcgcaatc tgataggttt gaccgtgata catcgccagc
    45301 gcctgaccgt aagcctgctt caggtactca cggaaagcgg caaagtaggc ttcacgttga
    45361 gcaggggtcg cactcttgta atactggccc agcaccagcg caccggcgta tttcacctgt
    45421 acgtatggca gcagttcctg atcaacaatg gtacgcagat aatccgggtt ggcccgaatt
    45481 tgcggttgct cattcttcag gcgatcgaac gttttctgcg ccgcctcgtc catcagctta
    45541 taaggattgg tctggtctgc cgcggttgcc gcactcagag gtgcaatcac cagcaaagcg
    45601 accatcatta aacgtttaaa catgcgtcgg ttctcctgaa attatttcgt tgtacccaca
    45661 ggttcagtgg tttcattatt acctggcgca gcagctggcg catcgccact attcttattg
    45721 tcatcgcctt tactaccgta aaggaactga ccaatgagat cttccagcac catcgcagac
    45781 ttggtgtcct gaattgtatc gccatccttc aggatagcag tccccagttc cgggtcttca
    45841 aaaccgacgt ttaatgccag atattgttct cccagcaggc cggaagtacg aatgctcagc
    45901 gaactggtat caggaatgtg gttataacgt tgttcaattt ccagcgttac gcgcggcaga
    45961 taggttttcg ggtccagcgt aatatccgcc acccggccca caacaacacc accaatgctg
    46021 accggagagc gcgctttcag gccgccaatg ttatcgaacg tcgcataaag cgtgtaggtc
    46081 gattctgtac gtatggacgt cacgttcgcc gccttcaggc aaacaaacag cgccgccagc
    46141 agtgctgcta ataaaaagat acccacccaa atttcatttt ttttcgtttg catgaactca
    46201 attcccaaac atcaatgcgg tcagcacaaa atccagcccc agaacagcca gagacgagtg
    46261 gacaacggtg cgagtggttg cccggctaat cccggcagac gtcgggatgg cgtcgtagcc
    46321 gttaaacaac gaaatccacg tcaccgtgat ggcgaacacc acgctcttaa tcagacagtt
    46381 gaccagatcc atacgccagt cgacggcatt ttgcattgcc gaccagaaga acccgctatc
    46441 aatgcctttc caactgacgc cgaccagcga tccgccccag atccccacgg cgacgaagat
    46501 aaccgtcaac agtggtaatg aaataacccc agcccagaaa cggggagaaa taacccgacg
    46561 cagcggatcc accgccatca tctccatact ggagagttgc tctgtagcgc gcatcaggcc
    46621 gatttctgcg gttaacgccg aaccagcacg cccggcaaac aacagcgcgg caaccaccgg
    46681 ccccagttca cgcagtagcg ataacgccac cagcataccc agactggttt ccgcgctata
    46741 agtggtcaga accagataac cttgcagccc caacaccatt ccgatgaaca cgccagaaac
    46801 tacaataatc agcatcgaca ggacgccgac attatagagc tggcgcacca gcagcggcgc
    46861 atgtttgcga aattccggtt tgccgaccag cgcattgaat aacattaacc cggcccgccc
    46921 gaacgttctc agggttttaa tccctttatg tccgagcgac gccagcgcat ttaacagcat
    46981 gagtggctta actccctggt aaaagatcag cgtgataatc gccggcagga tagcggaacg
    47041 gaacaggccc gtcagctatc ccgtccagaa actgacgtac gcgcggatca ggattcgtct
    47101 gcaacgcctg ggcactgcca tgagcgacaa tttttttgtc cgccaggatc caggcgtgat
    47161 ccgcaatact taacacttcc ggcacatcgt gagaaaccac cacacaagtc acgcccagcg
    47221 cgctgttcag ctcagaaatc agcttcacca gtacgcccat ggtgatggga tcttgcccaa
    47281 caaaaggttc atcaaacatg atgagatccg gctccagcgc aatcgcacgc gccagcgctg
    47341 cacgccgcgc catcccaccg gaaagttcag aaggcattag tttagccgct ccacgcagcc
    47401 ccacggcctc cagcttcatc atcaccgtac tatgcaacaa tggcgcggga agttgggtat
    47461 gttcgcgcag tggataggcg acgttgtcaa atacgttcat atcagtgaac aacgccccgg
    47521 actgaaataa catgctcatc cgtttacgca ctgtatacag gcgcgaacga gacatcgccg
    47581 gaatattctc accatcgaaa aggatctcac catgatctgg tgcgatttgc ccgccaatca
    47641 gacggagtag cgtcgtttta ccgatgcccg atggccccat gatcgccgtg atcttccctc
    47701 gcggcacggt cagggaaata ttatcgaaga tgcagcgatt gccacgcgta aaactgacat
    47761 cgcgcatatc gactaaattc gccacagact gctccataat tcacccttcg tcttgcgttg
    47821 attttctaag catggcgctc aatttaacct tgaacccaac atatttacag aatattaccc
    47881 gccgtggtta gcgaaagctg gcatttgttt tactttttag ccgcataaag tcaaaattaa
    47941 gaatccgtta cggctttctg aaaatcttca gcggaccggc gagtatacct gaagaaagga
    48001 cgttagatgc ttttagctac ggcactgtta attgttggtt tacttttggt cgtttacagt
    48061 gccgaccgcc tggtttttgc cgcgtctatt ctttgccgaa cctttggcat cccgccgctg
    48121 atcatcggca tgacggtggt cagtattggt acatcgttac cagaaatcat cgtctcgctt
    48181 gccgcgtctc tgcacgaaca acgcgattta gccgttggta cagccctcgg ctcaaacatt
    48241 atcaatatat tgctgatcct cggtctggct gcgctggttc gtccttttac cgtccattcc
    48301 gatgttctgc gccgtgaatt acccttaatg ttgttggtca gcgtggtggc cggttccgta
    48361 ctctatgacg gacaacttag tcgaagcgat ggtatctttc tcctctttct ggctgtgcta
    48421 tggctgctgt tcattgttaa acttgcacgt caggggactg acagcctgac cagagagcag
    48481 cttgcagagc tgccgcgtga cggcggattg cccgtcgcgt ttttatggct cggcattgcg
    48541 cttatcatca tgccagtggc cacgcggatg gtggttgata acgccacggt gctggcgaat
    48601 tactttgcca tcagcgagtt gacgatgggt ctgacggcaa ttgctatcgg aaccagtctg
    48661 ccggaactgg caaccgcaat agcgggggtt cgcaaaggtg aaaacgacat tgctgtcgga
    48721 aatatcattg gcgcaaacat ttttaatatt gtcatcgtgt tgggtttacc cgcgctgata
    48781 acgccaggag agattgatcc actggcgtac agtcgtgact acagcgtgat gttgctggtg
    48841 agcattattt ttgcgttgct gtgctggcgg cgctccccgc aaccgggccg tggtgtaggg
    48901 gtattattaa ctggcggatt tatcatatgg ctggcgatgt tgtactggtt atcgccaata
    48961 ctcgttgaat aactggaaac gcattatgtc gcacgtagag ttacaaccgg gttttgactt
    49021 tcagcaagca ggtaaagaag tcctggcgat tgaacgtgaa tgcctggcgg agcttgatca
    49081 atacatcaat cagaatttca cgcttgcctg tgaaaagatg ttctggtgta aagggaaagt
    49141 tgtcgtcatg gggatgggga aatcggggca catcgggcgc aaaatggccg caacgtttgc
    49201 cagcaccggt acaccttcat ttttcgtcca tcctagtgaa gccgcgcatg gtgatttagg
    49261 catggtcacc ccacaggatg tggtgattgc tatctctaac tcaggtgaat ccagcgaaat
    49321 cacggcctta attccagtgc ttaagcgtct tcacgtaccg ttaatctgca tcaccggtcg
    49381 cccggagagc agcatggcgc gcgccgcaga tgtgcatctg tgtgttaaag tagcgaaaga
    49441 agcctgtccg ttagggctgg caccgaccag cagcaccacc gccacgctgg ttatgggcga
    49501 tgccctcgct gtcgcgctgt taaaagcacg cggctttact gctgaagatt ttgcgctctc
    49561 acacccaggc ggcgcactgg gtcgtaaact tctgctgcgc gtaaacgata ttatgcatac
    49621 gggcgatgag atcccgcatg ttaagaaaac ggccagtctg cgtgacgcat tgctggaagt
    49681 tacccgcaaa aatcttggta tgactgtcat ttgcgatgac aatatgatga ttgaaggcat
    49741 ctttaccgac ggtgatttac gccgtgtctt cgatatgggc gtggatgttc gtcagttaag
    49801 tattgccgat gtgatgacgc cggggggaat acgtgtgcgc cctggcattc tggccgttga
    49861 ggcactgaac ttaatgcagt cccgccatat cacctccgtg atggttgccg atggcgacca
    49921 tttactcggt gtgttacata tgcatgattt actgcgtgca ggcgtagtgt aaagattcaa
    49981 ggataaacaa caatgagcaa agcaggtgcg tcgcttgcga cctgttacgg ccctgtcagc
    50041 gccgacgtta tggcaaaagc agagaacatt cgtctgctga tcctcgacgt tgatggcgta
    50101 ctgtcagatg gcctgattta tatgggcaat aacggcgaag agctgaaagc gttcaatgtt
    50161 cgtgacggtt atggcattcg ttgtgcgctc acctctgata ttgaagtcgc tatcattacc
    50221 gggcgaaagg ctaaactggt agaagatcgt tgtgccacat tggggatcac tcacttgtat
    50281 caggggcagt caaacaaact gatcgccttt agcgatttgc tggaaaaatt ggcgattgcc
    50341 ccggaaaatg tggcttatgt tggcgatgat ctcatcgact gcccggtaat ggaaaaagtg
    50401 ggtttaagcg tcgccgtggc cgatgcccat ccactgttga tcccgcgcgc cgattacgtg
    50461 acgcgcattg ctggcggtcg tggcgcagtg cgcgaagttt gcgacttatt actcctggcg
    50521 cagggcaaac tggatgaagc caaagggcaa tcgatatgag taaagccaga cgttgggtta
    50581 tcattgtgct atcactggcg gttctggtga tgatcggcat taatatggcc gaaaaagacg
    50641 ataccgccca ggtggtcgtc aacaacaatg atcccaccta taaaagcgag catacggaca
    50701 cgctcgtcta taacccagaa ggggcactaa gctatcgatt gattgctcaa cacgttgaat
    50761 attattccga tcaggccgtt tcgtggttta cgcagccggt acttaccacg tttgataagg
    50821 ataaaatccc gacatggtcc gtaaaagcag ataaagccaa gctgaccaat gaccggatgc
    50881 tctatttata tggacacgtt gaagtcaacg cacttgtgcc agactctcaa cttcgcagaa
    50941 tcacgacgga taacgcgcag atcaatctgg tgacgcagga tgttacctct gaagacctcg
    51001 tcacgttata cggaacaaca tttaactcca gcggtctgaa aatgcgcggc aacttacgca
    51061 gcaagaacgc cgagctgatt gaaaaggtta gaacatccta tgaaattcaa aacaaacaaa
    51121 ctcagcctta atcttgtgct tgccagctca cttctggccg ccagcattcc ggcatttgcc
    51181 gtaaccggag acactgatca gccgatccac attgaatcgg accagcaatc tcttgatatg
    51241 caaggcaacg tggttacctt taccggtaat gtcatcgtca cccagggcac catcaaaatt
    51301 aatgccgaca aagtggtcgt tacccgtccg ggcggcgaac aaggtaaaga agtgattgac
    51361 ggatacggta aaccggcaac gttctaccag atgcaggaca acggtaaacc cgttgaaggt
    51421 cacgcttccc agatgcacta cgaactggca aaagattttg tcgttctgac gggtaatgct
    51481 tatctgcagc aggtcgatag caacattaag ggcgataaga tcacttacct ggtgaaagag
    51541 cagaaaatgc aggctttcag cgacaaaggc aagcgcgtaa ccaccgttct ggtgccgtcg
    51601 cagctgcagg acaaaaacaa caaaggccag accccggcac agaagaaggg taattaattc
    51661 gttatggcaa cattaactgc aaagaacctt gcaaaagcct ataaaggccg tcgcgtggta
    51721 gaagacgtca gcctgaccgt caactccggg gaaattgtcg gtctgctggg gccaaacggt
    51781 gccggtaaga ccaccacttt ctacatggtt gtaggcattg tgccgcgcga tgcgggcaac
    51841 atcattattg atgatgacga tatcagtctg ctgcctctgc atgcacgcgc gcgccgcggt
    51901 atcggctatc tgccacagga agcctccatt ttccgtcgcc tcagcgttta cgataacctg
    51961 atggcggtac tgcaaattcg tgacgacttg tctgctgaac aacgtgaaga ccgcgcgaac
    52021 gagctgatgg aagagtttca cattgagcac ctgcgtgaca gcatggggca gtcactctcc
    52081 gggggtgaac gtcgccgtgt agaaattgcc cgcgcactgg ctgcgaatcc gaaatttatt
    52141 ctgctcgacg aaccgtttgc cggggttgac ccgatctcgg ttatcgatat taaacgcatc
    52201 attgaacacc tgcgcgacag cggcctgggc gtgctgatca ctgaccacaa cgtgcgtgaa
    52261 acactggcgg tttgtgaacg cgcttatatc gtcagtcagg ggcatttgat cgcccacggc
    52321 acgcctacag aaatcttaca agacgaacac gttaagcgtg tataccttgg ggaagacttc
    52381 agactctgat agggtagaag tttgcgacgt tttagcagga gagtacgatt ctgaacatga
    52441 agcaaggttt gcaactcagg cttagccaac aactggcgat gacgccacag ctccaacagg
    52501 caattcgtct gttgcagttg tcgacgctgg aacttcagca ggagctacag caggcgctgg
    52561 agagtaatcc gctgcttgag caaatcgaca ctcatgaaga aatcgacacc cgcgaaacgc
    52621 aagacagtga aacgctggac accgccgacg cgctcgaaca aaaagagatg ccggaagagc
    52681 tgccgctcga tgccagttgg gacaccattt acaccgctgg tacaccatcc ggcaccagcg
    52741 gtgactacat tgacgacgag ctgccggtct accagggcga aacgacgcag accttgcagg
    52801 attacctgat gtggcaggtc gagctgacac cgttttccga cactgaccgc gctattgcta
    52861 cctctatcgt cgatgccgtt gatgaaaccg gttatctgac tgtcccgctg gaagatattc
    52921 tcgaaagtat gggcgatgaa gagatcgaca tcgacgaggt tgaagccgtc cttaagcgga
    52981 tccaacggtt tgatccggtc ggtgtagcgg caaaagatct gcgtgactgc ctgctaatcc
    53041 aactctccca attcgataag accacgccgt ggctggaaga ggccagattg atcattagcg
    53101 atcatctcga tctgttagcc aatcacgact tccgcacttt aatgcgcgtc acgcgtctga
    53161 aagaagatgt gctgaaagaa gccgtcaatc tgatccagtc gctcgatccg cgccccgggc
    53221 agtcgatcca gactggcgaa cctgagtatg tcattccaga tgtgctggtg cgtaagcata
    53281 acggtcactg gacggtagaa ctcaacagtg acagcattcc gcgtctgcaa atcaaccagc
    53341 actacgcctc gatgtgcaat aacgcgcgca acgatggtga cagccagttt atccgcagca
    53401 atctgcagga tgccaaatgg ttgatcaaga gtctggaaag ccgtaacgat acgctactgc
    53461 gcgtgagtcg ctgtatcgtt gaacagcagc aagccttctt tgagcaaggt gaagaatata
    53521 tgaaaccgat ggtactggcc gatatcgccc aggctgtcga aatgcatgaa tcgacgatat
    53581 ctcgcgtgac cacgcaaaaa tacctgcata gtccacgagg catttttgaa ctgaagtatt
    53641 tcttttccag tcacgtcaat accgagggcg gcggcgaagc ttcctccacg gcgattcgtg
    53701 cactggtgaa gaaattaatc gcggcggaaa acccagcgaa accgttgagc gacagcaagt
    53761 taacctcttt gctgtcggaa caaggtatca tggtggcacg ccgcactgtt gcgaagtacc
    53821 gagagtcttt atccattccg ccgtcaaacc agcgtaaaca gctcgtttga cccaaccgat
    53881 aaggaagaca ctatgcagct caacattacc ggaaataacg tcgagatcac cgaggcactg
    53941 cgcgaatttg ttacagctaa atttgccaaa cttgagcaat attttgaccg aatcaaccag
    54001 gtctatgttg ttctgaaagt ggagaaagtc acccacacct cagatgcaac actgcatgta
    54061 aacggtggtg aaattcatgc cagcgcggaa ggtcaggaca tgtacgctgc cattgatggc
    54121 ttaattgata agctggcacg ccagttgacc aaacataaag ataaactgaa acaacactaa
    54181 ttgtccgggc aattagcatg tgcatggcgg cctgttgtgc ggcacaacgg gccatttgta
    54241 cggttaatgc tccgagcctg ttccactgtt tgagtgggca ggttcttagg tgaaattatg
    54301 acaaataatg atacaactct acagcttagc agtgttctta acagggaatg tacgcgaagc
    54361 cgcgtccact gtcagagcaa aaaacgcgcc ctggaaatca tcagcgagct ggcggcgaaa
    54421 caacttagcc taccgcctca ggtggttttt gaagctatcc tgacgcggga aaaaatgggc
    54481 agtaccggta tcggcaatgg tattgccatt ccgcatggca aacttgaaga agatactctg
    54541 cgtgccgttg gcgttttcgt tcagctcgaa acgcctatag ctttcgatgc catcgacaac
    54601 caaccggtgg acttactttt tgccctgctg gtgccggcag accaaactaa aacgcacctg
    54661 catactctgt cgctggtggc gaaacgtctg gcggacaaaa ccatctgccg ccgtttacgc
    54721 gcagcccaga gcgatgaaga gctgtatcaa atcattacgg ataccgaagg tactccggat
    54781 gaagcgtagt tattcggtaa tgtctctttt agacgttgtg aggagaaaca gtacatggta
    54841 ctgatgatcg tcagcggacg ttcaggttca ggtaaatctg tcgccctgcg tgcgctggaa
    54901 gatatgggtt tttactgcgt ggataacctt cccgtagtgt tgttacccga tctggctcga
    54961 actctggccg atcgcgagat ttctgccgcc gtcagcattg atgttcgtaa tatgccggag
    55021 tcaccagaaa tattcgaaca ggcgatgagt aacctgcctg acgctttctc accgcaacta
    55081 ctgttcctgg atgccgaccg taatacctta attcgtcgtt acagtgacac gcgccgactg
    55141 catccgcttt ccagcaaaaa cctgtcgctg gaaagtgcta tcgacaaaga aagcgatttg
    55201 ctggagcctc tgcgttcgcg agcggatctg attgtcgata cctcagaaat gtccgttcac
    55261 gagctggctg aaatgctgcg tacccgtctg ctgggtaaac gtgaacgcga actgaccatg
    55321 gtctttgagt ctttcggctt caaacacggt atccctatcg atgcagatta cgtctttgac
    55381 gtgcgcttct tgccgaaccc gcactgggat ccgaaactgc gtccaatgac aggtcttgat
    55441 aaacctgtcg ccgcgttcct cgaccgccac acagaagtac acaattttat ctaccagacg
    55501 cgaagctatc ttgagctatg gttacctatg ctggaaacca acaaccgtag ctacctgacg
    55561 gtcgccattg gttgtaccgg cgggaagcac cgttcggtgt atattgcaga gcaactggca
    55621 gactacttcc gctcgcgcgg taaaaacgtc cagtcacgcc atcgtacgct ggaaaaacgt
    55681 aaaccatgac cgtcaagcaa actgttgaaa tcacaaacaa gctgggcatg catgcccggc
    55741 ctgcaatgaa gctgtttgaa ttaatgcagg gttttgacgc tgaagtgctg ttacgtaatg
    55801 acgaaggcac cgaggctgaa gccaacagcg ttattgcgct gctgatgttg gattctgcca
    55861 aaggacggca gattgaagtt gaagcgaccg gtccacagga agaggaagca ctggccgccg
    55921 ttatcgccct ctttaattct ggttttgatg aagattaatc ttcatcactt tgacatacaa
    55981 ctcccttcaa gctcccccga caataagaaa atcacgtact gaaatcgttc tcaatcaacg
    56041 tcatttgtac attttgtgcg cttttcactt tcagaagaac cttaagaaaa ccttaagagg
    56101 cattgtttag gttttgttta agttaatcga ccatactgga gatcgtcaga aaatatttcc
    56161 aggagatggc atgattcgct tatctgaaca aagtcccctg ggcaccggaa gacatcggaa
    56221 atgttatgcg catccggaag atgcccaacg ctgtatcaag attgtctacc atcgtggcga
    56281 tggtggcgat aaagagatcc gccgtgagtt aaagtactac gcgcatcttg gtcgccggtt
    56341 aaaagactgg agtggaatac cgcgctatca cggtaccgtt gagacggatt gcggaaccgg
    56401 ttatgtctac gatgtgattg ccgattttga cggcaagcct tccattacgc tgaccgaatt
    56461 tgctgaacaa tgccgttacg aagaagacat tgcacaactg cgccagctac tgaaacagct
    56521 gaagcgttat ttgcaggata accgtatcgt gacgatgtcg ctgaagccgc agaatatcct
    56581 ctgtcaccgc atcagtgagt cagaggttat cccggtcgtc tgcgacaaca ttggtgaaag
    56641 cacattgatt ccactggcga cctggtcgaa gtggtgttgt ctgcgtaagc aagagcggtt
    56701 atggaaacga tttatcgccc aaccggcgct ggctattgcc ctgcagaaag atctgcaacc
    56761 tcgtgagagc aaaacgctgg ccctcacttc gcgcgaagct taatccagct ggtgttgttg
    56821 cataaacggc tcaccgccta actgatacat ctgccgtaaa atccacgcct gacggctacg
    56881 cacgtagccg tcaggcgtgg agactttgaa acgaagtgga ttaggtaata cagctgccag
    56941 taatgcagct tccgaccggg taagtttgct cgcgggtttg tggaaataac gttgtgccgc
    57001 agcttcgacg ccaaacacac cgtcgccaaa ttcggcgata ttcaggtaaa ccgtcaggat
    57061 acgctttttg ctccagactg tttctatccc cagcgttaat ccagcttcca gcccttttcg
    57121 tacccagcta cgcccatccc ataaaaagag atttttggcc gtctgctgag aaatcgttga
    57181 agcaccgcga atacggtttt cattgcgctc gttgtgcgcc agggctttct caatggaagc
    57241 gacatcaaag ccccagtgct caggaaattt ctgatcttct gcggcaatca ccgccagtcc
    57301 catccacggc gagatttgat ccatactgac ccagtcagaa tgtgccacgt aacgaaaatt
    57361 gccatgcagc caggcgctga cctgtcgctc gaccattacc gctgagaagg gaacaggcgc
    57421 aacgctaaac aacgcgatgc ccccgcccca gaaaacggcg aggacaacca ttaaccgcaa
    57481 aagaaaacgg cgaacgaaac taaacaccgt taagcggctt ttactcattc agccagaacc
    57541 agcacgcggg aaaccagctt atcaatgccg ctcgccgctt ctgcaatgtt ctgcgccagc
    57601 atatatgctg gggtggtgac aatcttattg tcttcatcaa ccacgatatc atcgacagga
    57661 cacggcacat gctccgcgcc catctcttcc agtacttctg cggtatcgat atcagtaccg
    57721 atggtcaaac gcagcgggaa atcgaaaatt ttcggcagca tcgccggggc aatacacata
    57781 aaaccaagcg gttttccggc ctgatgcatc gcttgtgcca gcgcctttaa ttcacggtca
    57841 acggtgcatt cgctaccaag actggcaaaa ttgcttaaat tcttcgccgc gccaaccccc
    57901 cccggcacaa tcaacgcatc cagttcagcg gcatcggcct gggccagagg acggatttca
    57961 ccgcgcgtta ttcgtgccgc ttcaatcagc acattgcgcg tttccgtcat cgcttcgcca
    58021 gttaaatggt tgataacatc aacctgctgc ttatccggtg caaagcagac cgcctgcgca
    58081 ccgctgcgtg aaatagctaa caacgtcaac accgcttcat gaatttcaga accgtcatag
    58141 acgccgcatc cgctcagaat tacgccaatt ttcttcattg tgatcatcct ttcgcaattg
    58201 actgaaacac attaattttt ttaataaaaa tggtacgcat cacacattta actgattcat
    58261 gtaacaaatc atttaagttt tgctatctta actgcgtgcg gcctgaaaaa cagtgctgtg
    58321 cccttgtaac tcatcataat aatttacggc gcagccaaga tttccctggt gttggcgcag
    58381 tattcgcgca ccccggtcta gccggggtca ttttttagtg gcttttgcca cccacgcttt
    58441 cagcacttct acgtcgtgac gccactcttc tttcatctct tcaatccatt caccgacgtt
    58501 atcttcccag gcgggaaggt caggagactg aatttgctga cccagctgtt gcagatggcg
    58561 taaccccact gaccccgccg cacctttaat tttatgtcct tcctcaacaa tgcctttttt
    58621 atcctgcgcc gtcagattcg actccagcac gctgacatag cccggcatca tcttctcaaa
    58681 caccgctaac ccgtcggtga tcagcttcgg tcctacaagt tcgagatact gttccagcat
    58741 gggaatatcg agcaatgctt ctgatttact gttctcttct gtcgtcaccg tactcttctc
    58801 atcatcctgg gtatcccaga atttcttgat catcgcggtt agcgccggaa ccgaaagcgg
    58861 cttactcagc acatcatcca ttccagcatt gaggtactct tgtttgtctt tcagcacgtt
    58921 agcggttaag gccaccagcg gcggtaaatc ctcgcgcgga taacgcttcg tcagttcacg
    58981 agagatatcc agcccggtca tatctggcaa ctgaatatcc agcaacacca ggtcgtattc
    59041 gcccggttta aacatctcca gcgccgcctt gccggtcatg gcgacatcaa cgctgttacc
    59101 taatttttcc agcacagaac gcgcgacaat cacgttcagt tcaatgtctt ccaccagcag
    59161 cacattcagc gccggtaaag gcatatcgtc ttcatcaaac gcatcatcga cctcttctgc
    59221 caccgacggt gcgtggatcg tcaacgtaaa ggttgaacct ttgccctgtt cgctggtaac
    59281 cgtaatatcg ccgcccatat ttttcgccag acgacgagaa acggccagac caataccggt
    59341 gccggtggca ggtttaccgc catgactgtc tttcacctgg taatacatgg cgaagatttt
    59401 atccagctca tcctgcggaa tgccgatacc agaatcttcc acttcaaaat gcagcatatc
    59461 gccttcatcg tagcgcacgc gcacggtaac ctggccttgc tgggtgaatt tgacggcgtt
    59521 actgatgagg ttccacagga tctgccgtaa acgcgtcccg tcggtaatga cctgatgcgg
    59581 taatggcagc gtaggctcca ggttaaagcg caatcctttt tgttgcgcct gcaatgcgga
    59641 gagattttcc agatcggcaa ggaagctggt gaaatcaacc ggctgattat caagctggac
    59701 cttgcgccgt tccatcttat ccatgtcgat aatatcgtta aagatattcc ccagcgtgac
    59761 ggccgaaaca tggatagttt tgagatattt ttcctgctcg gcggtgagtt cggtatccag
    59821 cagaatgcgg ctcaggccga cgataccgtt cagcggtgta cgcaattcgt gactgatggt
    59881 ggaaataaac gtcgttttgt cgcggctggc gcgttcaagc gcatcctgat accgcttacg
    59941 ctcggtaatg tcgcgaccaa agcccatcaa accgtgacgt ttacccacgc ggtcgtagta
    60001 cggcacttta cggatttcaa agcaggcttt gcgcccgtcc gggtaatcca gccactgttc
    60061 ataggtcagt gacacattat gacggaacac tttttcatcg gtttcaatga cttttgcggc
    60121 ggcttccggt gagtaaacat cagcaggttt cagatgaacc agttgttttt cgctttttcc
    60181 ggtcagcagc tccatcgcgc ggttacagcc ggaaaactct ttatcttcgt tacgataaaa
    60241 aaccaggtcg ggtgaagcat caaggaagga acgtaagaat gaggattgct gctcgagctg
    60301 aatttgtgtc tcttcgcgct ctttgatttc aattttcagt tggccgaagg tttcctgcag
    60361 ttctgcttcc gctttttcac gaacggcaat ttcctgattt agctgggcaa tattatcttt
    60421 taactgaacg ttgaggctca aatcgcgctc gcgcatctcc tccagttttt gcaccagccg
    60481 tgacagacgt tgtcgtgact cctccagttg ctcgacgacc accgatagaa agtagaccgc
    60541 ccacggcgta atcagcaaac caaagaagat agaacgaata acatcaatgc tttcgacctg
    60601 accatgcagc accatggtta ccgccatttg taccacaatg gcaagaacga cgagggccag
    60661 cgccagcaac attgagaagc gcaccagacc taacttcatc atcaggtcaa cataatactg
    60721 cgccagcaga cgaatttgct tcattaggga attccttcac gacaacctgc cccaataata
    60781 cccaattctg acagttacgt tataaattgt gtgagaaatg cggaattccc ctcaacgacc
    60841 tactccgttg gaggtagcca cggacgtccc agcgccgatc cctgtacgcc atgttcattc
    60901 agatagcgat ccagctcgac cattcccgtc catcgatttt cgcaccacag cggagcaagc
    60961 agcgtcgggc gacgggcgct ggcggaaatg cggtggtaga tcacttccgg tggcgtatgg
    61021 cgaatcattt ctccggcagt gagcgtataa tcctccagtt caataccgtt caaacgcccc
    61081 gcttcccagg ctttcgccat aatgctgcct ttcacaatat gcagcggatg cagctttatg
    61141 ccgtccacgc tggtttcaac cactcgctcc agcgtttgca aacattcagc ctgcccttcg
    61201 ccaggcaggc cgacaattaa gtgggaacac accttcagcc cgcgctgacg tgccagctgg
    61261 gttgtacgct gataacaggc aaaatcatgg ccgcggttga tgcgatgcag tgttttgtcg
    61321 tgggcggttt gtagccccag ctccagccac acttcgtaac cctggtcctt atattcgcaa
    61381 agcagatcca gcaccgcatc cggcacgcag tccgggcggg taccaacaca caaaccgaca
    61441 atattggcct ggctcaccgc ctgctgatac atagaacgca gcacctgaac ttccgcaaag
    61501 gtgctggtat acgcctgaaa gtaggccaga tagcgtttag cgcggttaac taaattcgcc
    61561 tggtgcgcca gttgctcggc aatggaacga tgctgctgcg cttcatcggc aaacgaggca
    61621 acattacaga atgtgcagcc accacgcccg atggtaccgt cacggttagg gcagctaaaa
    61681 ccgccgtgca gcgtcagctt atgcaccttt tgcccataac gacgggtgag atcaccacca
    61741 aacatattga ctaatttctg taactgcata atctgataga ccgcgccttg aaaagaggcc
    61801 aaagcctgcc atttttagct caattcggcg atgacctaga tcaatcgtcc cgccctgctt
    61861 ttatcaactg cataatcaat caaaattacc gaaatttcat gcataatcac ataaatcact
    61921 tttgcttatc ttgtgtcaga tttttttatc tccttatgga ttttaggcaa aaacagtagc
    61981 atgaaacgtc attaccaatt aaggcagtat aaaatgctgg ttttctcgtc agttcaaggc
    62041 aggataaggg ttaacacgcc tttatgacag tcaggaattg actgtttctc taacgactct
    62101 ccttttagcc ttaaagataa aatctatttt aatttcagcc atttaataaa gaattttgcg
    62161 ctaaagcaca tttctgtacc aataagcttg ccatttgacc tgtatcagct ttcccgataa
    62221 gttggaaatc cgctggaagc tttctggatg agcagcctgc tcatcatatt tatgcagtaa
    62281 ttgagatccc ctcttcaccg tattaaccga tgcgaaaagg acaacaaggg ggcgaatgcg
    62341 aggcgcgcgt atgacacgca aaccccgtcg ccacgctctt tctgtgcccg tgcgcagcgg
    62401 ttcggaagtg gggttcccgc agagcctggg ggaggttcac gatatgttgt acgataaatc
    62461 ccttgagagg gataactgtg gtttcggcct gatcgcccac atagaaggcg aacctagcca
    62521 caaggtagtg cgtactgcaa tacacgcact ggcccgcatg cagcaccgtg gcgcgattct
    62581 cgccgatggt aaaaccggcg acggttgcgg cttgctgtta caaaaaccgg atcgcttttt
    62641 tcgcatcgtt gcgcaggagc gcggctggcg tttagcaaaa aactacgctg tcgggatgct
    62701 cttcctgaat aaagatcctg aactcgccgc tgccgcacgc cgcatcgttg aagaagaact
    62761 gcaacgcgaa accttgtcga ttgtgggctg gcgtgatgtc cccactaacg aaggcgtgct
    62821 gggtgaaatc gccctctcct ctctgccacg cattgagcaa atttttgtga acgccccggc
    62881 aggctggcgt ccacgcgata tggagcgccg tctgtttatc gcccgccgcc gcattgaaaa
    62941 gcgtctcgaa gccgacaaag acttctacgt ctgtagcctg tcgaatctgg tgaacatcta
    63001 taaaggtctg tgtatgccgg cggatctgcc gcgcttttat ctggatcttg cggacctgcg
    63061 tctggaatcg gccatttgcc tgttccacca gcgcttctcc actaacaccg taccgcgctg
    63121 gccgctggcg caaccgttcc gctatctggc gcataacggt gaaatcaaca ccatcaccgg
    63181 taaccgccaa tgggcgcgtg cgcgtaccta taaattccag acgccgctga tccctgacct
    63241 gcacgacgcc gcgccattcg tcaacgaaac cggttctgac tccagttcca tggataacat
    63301 gctggaactg ctgctggcag gcgggatgga tatcatccgc gccatgcgcc tgttagtgcc
    63361 acccgcctgg cagaacaacc cggatatgga cccggaactg cgtgccttct ttgactttaa
    63421 ctccatgcat atggagccgt gggatggtcc ggcgggcatc gtgatgtccg atggtcgctt
    63481 cgctgcctgt aacctcgacc gtaacggtct gcgtccggca cgctacgtca ttaccaaaga
    63541 caagctcatc acctgcgcct ctgaagtcgg tatctgggat taccagcctg acgaagtggt
    63601 cgaaaaaggc cgcgtcgggc caggcgaact gatggtgatc gacactcgca gtgggcgtat
    63661 tctgcactcg gcagaaaccg atgacgatct gaaaagccgc cacccttata aagagtggat
    63721 ggagaaaaac gtccgccgac tggtaccgtt tgaagatctg cccgatgaag aagtgggtag
    63781 ccgcgaactg gacgacgaca cgcttgccag ctaccagaaa cagtttaact acagcgcgga
    63841 agagctggac tccgtaattc gcgtactggg cgaaaacggt caggaagcgg tcggttcgat
    63901 gggcgatgat accccattcg ccgtgctctc cagtcagccg cgcattattt acgactactt
    63961 ccgccagcag tttgcccagg tgactaaccc gccaatcgac ccgctgcgtg aagcgcatgt
    64021 tatgtcgctc gccaccagta tcggtcgtga aatgaacgtc ttttgcgaag cagagggcca
    64081 ggcgcaccgt ttaagcttta aatcgccgat tctgctctac tccgatttca aacagctcac
    64141 gacgatgaaa gaggagcact accgcgcaga tacgctggat atcacctttg acgtcactaa
    64201 aaccacgctc gaagcgacag tcaaagagct atgcgacaaa gccgaaaaaa tggtacgtag
    64261 cggcaccgtg ttgctggtgc tctccgaccg gaatatcgct aaagatcgcc tgccggttcc
    64321 ggctccgatg gcggttggcg cgatccagac ccgtctggtc gatcaaagcc tgcgttgcga
    64381 tgccaacatc atcgtcgaaa ccgccagcgc ccgcgatccg caccacttcg ccgtgttgct
    64441 gggtttcggc gcgacggcta tttatccgta ccttgcctat gaaacgctgg gccgcctggt
    64501 agacacccac gcgattgcca aagattatcg taccgtgatg ctcaactacc gtaacggcat
    64561 caacaaaggc ttgtacaaaa tcatgtccaa aatgggcatc tccaccatcg cctcttaccg
    64621 ctgctcgaaa ctgtttgaag cggtcggtct acacgatgat gtagtgggcc tgtgcttcca
    64681 gggcgcggtc agccgcattg gtggggcaag ctttgaagac ttccagcagg atctgctgaa
    64741 tctgtcgaaa cgtgcctggc tggcgcgcaa gccgattagc cagggcggcc tgctgaaata
    64801 cgtccacggc ggcgaatacc acgcctacaa cccggacgtg gtgcgcacgc tgcaacaggc
    64861 ggtacaaagc ggcgagtaca gcgactatca ggaatacgcg aagctggtta atgagcgtcc
    64921 gacaaccacg ctgcgcgatc tgctggcaat tacgccgggt gaaaacgcgg tcaacattgc
    64981 tgatgttgaa ccggcaagcg aactgtttaa acgctttgat accgccgcga tgtctatcgg
    65041 cgcgttaagc ccggaagccc acgaggcgct ggcggaagcg atgaatagca tcggcggtaa
    65101 ctcgaactcc ggtgaaggcg gcgaagaccc ggcgcgctat ggcactaaca aagtgtcgcg
    65161 catcaagcag gtagcttctg gtcgcttcgg cgttactccg gcgtatctgg tcaatgccga
    65221 cgtcattcag attaaagtcg cccagggcgc gaagccgggc gaaggcggtc agttgccggg
    65281 cgacaaagtc actccttaca tcgccaaact gcgctattcg gtgcccggag tgacgctgat
    65341 ctccccgccg ccgcaccacg atatctactc tatcgaagac ttagcgcagc ttattttcga
    65401 cctcaagcag gttaacccga aagcgatgat ctccgttaag ctggtgtccg aaccaggcgt
    65461 tggcaccatc gcgactggcg tggcgaaagc ttatgcggac ttgatcacca tcgcgggcta
    65521 tgacggcggc accggcgcaa gcccgctgtc gtcagtgaaa tatgcaggct gcccgtggga
    65581 actcggtctg gtggaaaccc aacaggcgct ggttgccaac ggcttgcgtc ataaaattcg
    65641 tttgcaggtt gatggcggcc tgaaaacggg tgtcgatatc atcaaagcgg cgattctcgg
    65701 cgcagaaagc ttcggcttcg gcactggccc aatggtggcg ctcggctgta aatatctgcg
    65761 tatttgccac ctgaacaact gcgccacggg tgtagcaact caggatgaca aactgcgtaa
    65821 gaatcactat cacggcctgc cgttcaaggt gactaattac tttgagttta tcgcccgtga
    65881 aacccgcgag ctgatggcac agctgggagt aacgcgtctg gtggatctga ttggtcgcac
    65941 cgacctgctg aaagagctgg acggtttcac cgccaaacag cagaaactgg cgctgtcgaa
    66001 gctgctggag actgccgagc cacatgcagg taagtcactc tactgcactg aaaacaaccc
    66061 gccgtttgat aacggcctac tgaacgcgca gttgctgcaa caggcgaaac cgtttgtcga
    66121 tgaacgccag agcaaaacct tctggttcga tatccgcaac accgaccgtt ctgtcggcgc
    66181 gtcgctttca ggctatatcg cccagacgca cggcgatcag gggctggcag ccgatcctat
    66241 caaagcgtac ttcaacggca ccgcaggcca gagtttcggt gtgtggaacg cgggcggcgt
    66301 ggaactatac ctgaccgggg atgccaacga ctatgtcggt aaaggcatgg cgggcggctt
    66361 aatcgccatt catcctccgg ttggttccgc cttccgcagc catgaagcaa gcattatcgg
    66421 caacacctgc ctgtatggcg cgaccggtgg tcgtctgtat gccgcaggcc gcgcgggtga
    66481 acgtttcggc gtgcggaact ccggtgctat caccgtggta gaaggcattg gcgacaacgg
    66541 ctgtgaatat atgacgggcg gtattgtctg tattctgggt aaaaccggcg ttaacttcgg
    66601 cgcgggcatg accggcggct tcgcttacgt tctcgatgaa agcggtgatt tccgcaaacg
    66661 cgttaacccg gaactggtcg aagtcttaag cgtcgacgat ctggcgatcc atgaagagca
    66721 tctgcgcggt cttatcaccg agcatgttca gcataccggt tctcagcgcg gtgaagagat
    66781 tctggcgaac tggtcaacct tcgccacgaa attcgcgctg gttaaaccga agtccagtga
    66841 tgtaaaagca ctgctgggtc accgtagtcg tagcgcagca gagttgcgcg tgcaggcgca
    66901 gtaaggggta gcaacaatga gtcagaatgt ttatcaattt atcgacctgc agcgcgttga
    66961 tccgccaaag aaaccgctga agatccgtaa aattgagttt gttgaaattt acgagccgtt
    67021 ttccgaaggc caggccaaag cgcaggctga ccgctgtctg tcgtgcggca acccatactg
    67081 cgagtggaaa tgcccggtac acaactacat cccaaactgg ctgaagctcg ccaacgaggg
    67141 gcgtattttt gaagcggcag aactgtcgca tcagaccaac acactgccgg aagtatgcgg
    67201 acgtgtctgc ccgcaagacc gtctgtgcga aggttcctgc accctgaacg atgagttcgg
    67261 tgcggtgacc atcggcaaca ttgagcgtta tatcaatgat aaagcgttcg aaatgggctg
    67321 gcgtccggat atgtccggcg tgaagcaaac cggtaaaaaa gtggcgatta tcggtgcagg
    67381 cccggcaggt ctggcgtgtg cggatgtcct gacgcgtaac ggcgtaaaag ccgttgtctt
    67441 cgaccgtcat ccagaaattg gcgggctgct gaccttcggt attccggcct tcaagctgga
    67501 aaaagaggta atgacgcgtc gccgtgaaat cttcaccggc atgggtattg aattcaaact
    67561 caataccgaa gtgggccgcg acgtacagct ggacgatctg ctgagtgatt acgatgccgt
    67621 gttccttggc gttgggactt atcagtcaat gcgcggcggg ctggaaaacg aagacgccga
    67681 tggcgtgtac gcagcgctgc cgttccttat cgccaacacc aaacagttaa tgggctttgg
    67741 tgaaacccgc gacgaaccgt tcgtcagcat ggaaggcaaa cgcgtggtag tacttggcgg
    67801 tggcgacact gcgatggact gcgtgcgcac gtctgtacgc cagggggcaa agcacgttac
    67861 ctgtgcctat cgtcgtgatg aagagaacat gccgggttcc cgccgcgaag tgaaaaacgc
    67921 gcgggaagaa ggcgtagagt tcaaattcaa cgtccagcca ctggggattg aagtgaacgg
    67981 taacggcaaa gtcagcggcg taaaaatggt acgtaccgaa atgggcgaac cggatgccaa
    68041 aggccgtcgc cgcgcggaga tcgttgcagg ttccgaacat atcgttccgg cagatgcggt
    68101 gatcatggcg tttggtttcc gtccacacaa catggaatgg ctggcaaaac acagcgtcga
    68161 gctggattca caaggccgca tcatcgctcc ggaaggcaac gacaacgctt tccagaccag
    68221 caacccgaaa atctttgctg gcggcgatat cgtccgtggt tccgatctgg tcgttaccgc
    68281 catcgccgaa ggtcgcaaag cggcagacgg cattatgaac tggctggaag tttaagctct
    68341 gtcccagcgc cgcctcctca gaaaagagtc ggcgctgaaa ttctcctttc tttctggaaa
    68401 tcgcctcgca taatctgaag aaaaccaatg taatacgctc atctggattg ttaccggtag
    68461 atgcccggcg tatagctgat tccattattc aacgcagcgc catataaagg gacagataat
    68521 ggaatcgctc tcagagagaa cctcaacagg ctaccagcaa atccacgacg gcattattca
    68581 tctggtcgat agcgcccgaa cggaaacggt acgtagcgtt aacgcgctga tgaccgcgac
    68641 gtactgggaa attggccgac ggattgtcga atttgaacaa ggtggtgagg ccagagcagc
    68701 ttatggtgca cagttgattg atcggctatc gaaggattta agtcgacggt ataaacgtgg
    68761 ggtctctgca aaaaacttac gccaaatgag gcttttttac ctcttttttc aacatgttga
    68821 aattcgccag acagtgtctg gcgaattaac accattggga attccgcaga caccatctgc
    68881 agaattccca tcggcaaaga tttggcagac gctgtctgcc aaattgagag gctgtttgat
    68941 atttccacag cttttgtatt gcatctccta ttttcggttt gtatattcat gcaaataaac
    69001 gaagg
//
LOCUS       DADMGC010000004        68195 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.004, whole genome shotgun sequence.
ACCESSION   DADMGC010000004 DADMGC010000000
VERSION     DADMGC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 68195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68195
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.004"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..247
                     /locus_tag="JNZ73_000271"
     CDS             <1..247
                     /locus_tag="JNZ73_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8730686.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="JNZ73_000272"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="JNZ73_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HAY8730687.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWLIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="JNZ73_000273"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="JNZ73_000273"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY8730688.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="JNZ73_000274"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="JNZ73_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HAY8730689.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTMLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMVNVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3543)
                     /locus_tag="JNZ73_000275"
                     /pseudo
     CDS             complement(3258..3543)
                     /locus_tag="JNZ73_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3629..4444)
                     /gene="ybjI"
                     /locus_tag="JNZ73_000276"
     CDS             complement(3629..4444)
                     /gene="ybjI"
                     /locus_tag="JNZ73_000276"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HAY8730690.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMKNAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4444..5652)
                     /locus_tag="JNZ73_000277"
     CDS             complement(4444..5652)
                     /locus_tag="JNZ73_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8730691.1"
                     /translation="MTVNSSRNALKRRTRALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5736..6272
                     /locus_tag="JNZ73_000278"
     CDS             5736..6272
                     /locus_tag="JNZ73_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY8730692.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6447..8132)
                     /locus_tag="JNZ73_000279"
     CDS             complement(6447..8132)
                     /locus_tag="JNZ73_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HAY8730693.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAITNVLLTLAGTIIVMVWPGLG"
     gene            8402..8779
                     /locus_tag="JNZ73_000280"
     CDS             8402..8779
                     /locus_tag="JNZ73_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8730694.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8809..9066)
                     /gene="grxA"
                     /locus_tag="JNZ73_000281"
     CDS             complement(8809..9066)
                     /gene="grxA"
                     /locus_tag="JNZ73_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HAY8730695.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9226..9513
                     /locus_tag="JNZ73_000282"
     CDS             9226..9513
                     /locus_tag="JNZ73_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HAY8730696.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVVKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9497..10220
                     /gene="nfsA"
                     /locus_tag="JNZ73_000283"
                     /pseudo
     CDS             9497..10220
                     /gene="nfsA"
                     /locus_tag="JNZ73_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
     gene            10281..11183
                     /gene="rimK"
                     /locus_tag="JNZ73_000284"
     CDS             10281..11183
                     /gene="rimK"
                     /locus_tag="JNZ73_000284"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HAY8730697.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11271..11747
                     /locus_tag="JNZ73_000285"
     CDS             11271..11747
                     /locus_tag="JNZ73_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HAY8730698.1"
                     /translation="MTSLVVPGLDTLRQWLDGLGMSFFECNNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12099..13211
                     /gene="potF"
                     /locus_tag="JNZ73_000286"
     CDS             12099..13211
                     /gene="potF"
                     /locus_tag="JNZ73_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HAY8730699.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13306..14382
                     /gene="potG"
                     /locus_tag="JNZ73_000287"
     CDS             13306..14382
                     /gene="potG"
                     /locus_tag="JNZ73_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HAY8730700.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPT"
     gene            14449..15402
                     /gene="potH"
                     /locus_tag="JNZ73_000288"
     CDS             14449..15402
                     /gene="potH"
                     /locus_tag="JNZ73_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HAY8730701.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15399..16244
                     /gene="potI"
                     /locus_tag="JNZ73_000289"
     CDS             15399..16244
                     /gene="potI"
                     /locus_tag="JNZ73_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HAY8730702.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16304..16792
                     /locus_tag="JNZ73_000290"
     CDS             16304..16792
                     /locus_tag="JNZ73_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HAY8730703.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16833..17960
                     /gene="rlmC"
                     /locus_tag="JNZ73_000291"
     CDS             16833..17960
                     /gene="rlmC"
                     /locus_tag="JNZ73_000291"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HAY8730704.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18135..18866)
                     /gene="artJ"
                     /locus_tag="JNZ73_000292"
     CDS             complement(18135..18866)
                     /gene="artJ"
                     /locus_tag="JNZ73_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8730705.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19158..19826)
                     /gene="artM"
                     /locus_tag="JNZ73_000293"
     CDS             complement(19158..19826)
                     /gene="artM"
                     /locus_tag="JNZ73_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HAY8730706.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19826..20542)
                     /gene="artQ"
                     /locus_tag="JNZ73_000294"
     CDS             complement(19826..20542)
                     /gene="artQ"
                     /locus_tag="JNZ73_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HAY8730707.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20549..21280)
                     /gene="artJ"
                     /locus_tag="JNZ73_000295"
     CDS             complement(20549..21280)
                     /gene="artJ"
                     /locus_tag="JNZ73_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8730708.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21298..22026)
                     /gene="artP"
                     /locus_tag="JNZ73_000296"
     CDS             complement(21298..22026)
                     /gene="artP"
                     /locus_tag="JNZ73_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HAY8730709.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22244..22759)
                     /locus_tag="JNZ73_000297"
     CDS             complement(22244..22759)
                     /locus_tag="JNZ73_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8730710.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23408..25177
                     /locus_tag="JNZ73_000298"
     CDS             23408..25177
                     /locus_tag="JNZ73_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY8730711.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNAFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25388..25711
                     /locus_tag="JNZ73_000299"
     CDS             25388..25711
                     /locus_tag="JNZ73_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAY8730712.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25708..26538
                     /locus_tag="JNZ73_000300"
     CDS             25708..26538
                     /locus_tag="JNZ73_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAY8730713.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26535..27548)
                     /locus_tag="JNZ73_000301"
     CDS             complement(26535..27548)
                     /locus_tag="JNZ73_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY8730714.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVLTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIAHSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27647..29077)
                     /locus_tag="JNZ73_000302"
     CDS             complement(27647..29077)
                     /locus_tag="JNZ73_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8730715.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANKHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29088..30089)
                     /gene="ltaE"
                     /locus_tag="JNZ73_000303"
     CDS             complement(29088..30089)
                     /gene="ltaE"
                     /locus_tag="JNZ73_000303"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAY8730716.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGAHIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30126..31844)
                     /gene="poxB"
                     /locus_tag="JNZ73_000304"
     CDS             complement(30126..31844)
                     /gene="poxB"
                     /locus_tag="JNZ73_000304"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HAY8730717.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31977..32945)
                     /gene="hcr"
                     /locus_tag="JNZ73_000305"
     CDS             complement(31977..32945)
                     /gene="hcr"
                     /locus_tag="JNZ73_000305"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HAY8730718.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32957..34609)
                     /gene="hcp"
                     /locus_tag="JNZ73_000306"
                     /gene_synonym="priS"
     CDS             complement(32957..34609)
                     /gene="hcp"
                     /locus_tag="JNZ73_000306"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAY8730719.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPSFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(34753..35652)
                     /gene="lysO"
                     /locus_tag="JNZ73_000307"
     CDS             complement(34753..35652)
                     /gene="lysO"
                     /locus_tag="JNZ73_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HAY8730720.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36109..36804)
                     /gene="aqpZ"
                     /locus_tag="JNZ73_000308"
     CDS             complement(36109..36804)
                     /gene="aqpZ"
                     /locus_tag="JNZ73_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HAY8730721.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     VSGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            37229..38887
                     /locus_tag="JNZ73_000309"
     CDS             37229..38887
                     /locus_tag="JNZ73_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAY8730722.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(38884..39840)
                     /locus_tag="JNZ73_000310"
     CDS             complement(38884..39840)
                     /locus_tag="JNZ73_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HAY8730723.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            39991..41106
                     /gene="macA"
                     /locus_tag="JNZ73_000311"
     CDS             39991..41106
                     /gene="macA"
                     /locus_tag="JNZ73_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HAY8730724.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMGGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            41103..43049
                     /gene="macB"
                     /locus_tag="JNZ73_000312"
     CDS             41103..43049
                     /gene="macB"
                     /locus_tag="JNZ73_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HAY8730725.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(43122..43346)
                     /gene="cspD"
                     /locus_tag="JNZ73_000313"
     CDS             complement(43122..43346)
                     /gene="cspD"
                     /locus_tag="JNZ73_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HAY8730726.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            43669..43989
                     /gene="clpS"
                     /locus_tag="JNZ73_000314"
     CDS             43669..43989
                     /gene="clpS"
                     /locus_tag="JNZ73_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HAY8730727.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            44020..46296
                     /gene="clpA"
                     /locus_tag="JNZ73_000315"
     CDS             44020..46296
                     /gene="clpA"
                     /locus_tag="JNZ73_000315"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HAY8730728.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(46699..46786)
                     /locus_tag="JNZ73_000316"
     tRNA            complement(46699..46786)
                     /locus_tag="JNZ73_000316"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46750..46752),aa:Ser,seq:gga)
     gene            complement(47040..47258)
                     /gene="infA"
                     /locus_tag="JNZ73_000317"
     CDS             complement(47040..47258)
                     /gene="infA"
                     /locus_tag="JNZ73_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAY8730729.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(47543..48247)
                     /gene="aat"
                     /locus_tag="JNZ73_000318"
     CDS             complement(47543..48247)
                     /gene="aat"
                     /locus_tag="JNZ73_000318"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HAY8730730.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(48289..50010)
                     /gene="cydC"
                     /locus_tag="JNZ73_000319"
     CDS             complement(48289..50010)
                     /gene="cydC"
                     /locus_tag="JNZ73_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HAY8730731.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTQLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(50011..51777)
                     /gene="cydD"
                     /locus_tag="JNZ73_000320"
     CDS             complement(50011..51777)
                     /gene="cydD"
                     /locus_tag="JNZ73_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HAY8730732.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(51900..52865)
                     /gene="trxB"
                     /locus_tag="JNZ73_000321"
     CDS             complement(51900..52865)
                     /gene="trxB"
                     /locus_tag="JNZ73_000321"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAY8730733.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            53410..53904
                     /gene="lrp"
                     /locus_tag="JNZ73_000322"
     CDS             53410..53904
                     /gene="lrp"
                     /locus_tag="JNZ73_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HAY8730734.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            54039..58145
                     /gene="ftsK"
                     /locus_tag="JNZ73_000323"
     CDS             54039..58145
                     /gene="ftsK"
                     /locus_tag="JNZ73_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY8730735.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFLGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSIFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREARRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFTQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            58300..58911
                     /gene="lolA"
                     /locus_tag="JNZ73_000324"
     CDS             58300..58911
                     /gene="lolA"
                     /locus_tag="JNZ73_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HAY8730736.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            58922..60265
                     /locus_tag="JNZ73_000325"
     CDS             58922..60265
                     /locus_tag="JNZ73_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAY8730737.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            60356..61648
                     /gene="serS"
                     /locus_tag="JNZ73_000326"
     CDS             60356..61648
                     /gene="serS"
                     /locus_tag="JNZ73_000326"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAY8730738.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            61888..64332
                     /gene="dmsA"
                     /locus_tag="JNZ73_000327"
                     /pseudo
     CDS             61888..64332
                     /gene="dmsA"
                     /locus_tag="JNZ73_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            64343..64960
                     /gene="dmsB"
                     /locus_tag="JNZ73_000328"
     CDS             64343..64960
                     /gene="dmsB"
                     /locus_tag="JNZ73_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY8730739.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     HYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            64962..65825
                     /locus_tag="JNZ73_000329"
     CDS             64962..65825
                     /locus_tag="JNZ73_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase"
                     /protein_id="HAY8730740.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(65861..66487)
                     /locus_tag="JNZ73_000330"
     CDS             complement(65861..66487)
                     /locus_tag="JNZ73_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY8730741.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            66802..67950
                     /locus_tag="JNZ73_000331"
     CDS             66802..67950
                     /locus_tag="JNZ73_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8730742.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT        16945 a        16862 c        17857 g        16531 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taagccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccatg ttgcgcttct tgcagggcat
     2341 aagcctctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggt gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccac
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcaca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga cacgcttgtg gaacgctgac
     3421 tgaatcagga accacaacca ctttgtatcc actcggcaca tcgacggtga cactttgcgc
     3481 catcgccaca ccagtaaagc ccattaaaag cgcgcccaac agtagacaat ttttcataat
     3541 catttcgcct gaaataatgc agcgggttaa tttccgcgaa ttatgcagcc ttcattcagg
     3601 cattgtgttg ccgggcgtgc tgccgttttt attggtcaaa tggcgcttcg tgtttaagaa
     3661 ctttatcgat cacatccagt acgccttcac ggttattgga gcctgcccgg tattttgccg
     3721 ctgcgacgac cgcgctgccg gcatttttca ttgcaaaact aaagcctgcc tgacgcagca
     3781 tctcaatatc gttaccgcca tcgccaaaga ccaccacttc gctgtcgtct attccccata
     3841 atttctgcag ttggcgaagg ccattggctt tatgtacgcc ggggataatc agatcgatgc
     3901 tgccgttgcc ggtgtggact ggcaccataa tatcgccgat ggcctcatgt aatgcttttt
     3961 gtacttgtgg aatcagttca tcggaaagat tcaggccaaa cttaaagaaa atatcttcta
     4021 agttgtcaaa gttatcgacg tattccagac ggtgataata catttccgcc accgttttca
     4081 tggcatcgtc atattttttg agtgtatagg cgctattttt tccgcaggca ataatttcca
     4141 cttccgggcg cgtcagcaaa tgttccacga cagtagcaaa cgcatccttc gacagctcgc
     4201 cattaaaaac atctttgcct tcactcacta cccagccgcc gttttcagcc acaaaggcaa
     4261 tttcattcgc aatttcaggg aagaaagaga tcaactgata atattgattc ccgctggcga
     4321 ccacaaagcg aatgccttgt gctttcattt gctgatactg agccataaac cgctcacggt
     4381 tataggtttt ttgatcgctt aagaaagtac catccatgtc taccgcaatt aatttaatac
     4441 tcatcaacta ttctccatcg ccgtctgcgt tttggtatcg ggtttggcga cggctttcgc
     4501 gacaatagcc gcgagaataa ccagcgccag tacaaccagc attgcactac gtaatccata
     4561 atgttcgccg agatagccca gcagcggcgg cccgacgagg aaagccagat aaccggtcgt
     4621 tgctaccaca ctgacgcggg ttggtgcatc ggggccggta tcgctggcgg cagaaatggt
     4681 cagcgggaag cccagcgagg cacccagtcc ccaaagtaca acagacactc ctgcgaccca
     4741 ggcgctatcg acaaaaataa tcagcccaat acccaacgcc cccattagtg cactggcccg
     4801 aaccacggca acgcgactgt aacggtcgat gaaccaaccg ccggtgaagc gtccaacggt
     4861 catccccagg gtaaaaccgg cataaatcag tgagccggaa gtggggctaa aaccgtgacc
     4921 atcaaccatt aataagggta accagtcgtt ggcagaacct tcggcaaagg ccatcgccag
     4981 cactacaaca ccaatcaaca gcaactggat atcgcgataa aaaggaatgc ctttttcgcc
     5041 atgctgggtg ccatcggcag catttttgcc cgtaccgtca gggattgcct gaatggcgat
     5101 ataaataggc gcgataccta ccagcgccgc caataaaatg tgcaccgttg ccggaacgcc
     5161 aaaggccgtc agtgccatcc cgacaccagc gcctgccagc gtgcccaggc tataaaaacc
     5221 gtgcatcatc ggcaaaaccg ttttattcat ttctcgctca acggcggcac cttcaacgtt
     5281 tatcgccact tccgcagaac caaaacttgc cccaaagacg ccgagaccaa cggcaaagag
     5341 caggggcgat gtcagccaga gtgccagact taatatcatc atcccgatca atgcgcagga
     5401 catcgtgacc aggatgacat tacgtgtccc aaagcgtttc actaaccacg ccgagcagag
     5461 aataccgctc atcgaaccga tcgacagacc aaagagaaca ccgcccattt cagcgatcga
     5521 gacagagaga atatcgcgga tagcaggcgt acgggttgcc caggacgcca ttaacaggcc
     5581 tggcaaaaag aagaacataa acagcgccct ggttcggcgt ttcaatgcat tacgtgaaga
     5641 atttacggtc atagatcacg tcaaaataag aagagggaag acaacattag caaggttgtg
     5701 tacatttgta cacaattgca gacagaggaa atgacatgcg tcgcgctaac gatccgcaac
     5761 ggcgagaaaa aattatccag gccacgctgg aggcggtgaa actttacgga atacatgctg
     5821 ttacgcaccg caaaattgct acccttgccg gggtaccgtt gggatcgatg acctactatt
     5881 tttcaggaat tgatgagttg ttgctggaag cgttcagcag ttttactgag attatgtccc
     5941 ggcaatatca ggcatttttt agcgatgtta gtgatgctca aggcgcatgc caggctatca
     6001 ccgatatgat ctacagctca caggttgcaa cgccggataa tatggagctg atgtaccagc
     6061 tctacgcgct ggctagccga aaaccgctat taaaaacggt aatgcaaaac tggatgcagc
     6121 gcagtcagca aacgctcgaa caatggtttg aacccggaac cgcccgcgcg cttgatgcgt
     6181 ttattgaggg gatgacgctg cattttgtca ccgaccgtaa gccgctatcg cgcgaggaga
     6241 ttttgaggat ggttgagagg gttgcagggt agtagataaa ttttagataa caaaaaaccc
     6301 atcaaccttg aaccgaaatg gcggggttga tgggctccac aaaatgggga catcaaagaa
     6361 aagcagtggc actaattaag actgatgccc tgcggaaaag ttctgcggtt gtgcaaaaaa
     6421 atttcatttt cagggcaact tcagttttat cctaatcctg gccataccat gacgatgatt
     6481 gtccctgcca gcgtcagcag gacgttggtg attgcgtagg tgcccgcata gcccagcgca
     6541 gggatgttac tgcgagctgt atcactgatg atctccattg ccggcgcgca ggtgcgtgcg
     6601 cccatcattg cgccgaacaa cagtgcgcgg ttcattcgca atacataagc accgaacaag
     6661 aaacagataa ccacgggcac aagactgaca attaatccgg caatcaacat ctgaccgcca
     6721 atcgcgccca ggccgttatt aataccgcta ccggcgctca gaccaacgcc tgccataaac
     6781 accatcaagc cgaactcttt caccatgctt aatgcacctt gcggaatgta accgaaggtc
     6841 gggtggttag cacgcataaa gcccagcata attccggcga ataacaaccc ggcagcgttc
     6901 cccatgccga aactgaatgt gctgaactgg aaggtgatca tcccgatcat cagcccaata
     6961 acaaagaagg cgcaaaatgc cagcaggtca gtgacctggc tgtgaatcga gataaagccg
     7021 atgcgatcgg cgatggtttt tacgcgacgg gcatcaccgc tgacttgtaa aacgtcacct
     7081 ttgttaagca cgacgttgtc atctatcggc atctcaatct ggctacgaat gacgcggtta
     7141 aggaagcaac cgtgatcggt caacttcagt tgtgcgagac gtttacctac agcgttatgg
     7201 tttttaacga ccacttcttc agtgacgata cgcatgtcga gaaggtcacg atcgaaaact
     7261 tctttaccgt tacggaagct gggatcgagt cgggcatggg cgtcgggata gcctaccaac
     7321 gctatttcat cgcccatttg tagcacggca tcaccgtctg gatttgccag aatcccgtta
     7381 cgtcgaatac gttcaatgta gcagccggtt tgtcgataaa tacccagttc acgcagattt
     7441 ttgccgtcgg tccaggccac cagctccggg ccgacgcgat aggcgcggat caccggtaaa
     7501 taaaccttac ggttggcatc agtgtccagg ccacgttcgc gggcgatttg ctgggcgctg
     7561 gtctgtaagt cctgatgctg caatttcggc aagtaacgcg caccaacaat caaactcacc
     7621 agaccgatta aataggttaa ggcatacccg aggctcagat tatccagtgc cagtgagagc
     7681 tgcctgcttt ccatgccgga atgatgcagt gtatcgccag caccgaccag aaccggtgtc
     7741 gacgtcatag agcctgctaa cataccggcc gtcaggccaa tatcccagcc aaacagctta
     7801 cctaacccta aggcgatcac cagcgcactg ccaaccatca ccagtgctaa cattaggtaa
     7861 tttttcccat cgcgaaaaaa aatggaaaaa aagttcggtc cggcttcgac cccgacgcag
     7921 aaaataaaca gcataaagcc aagattaagc gcatcggtgt taatgctgaa atgttgttgg
     7981 cctaataaca gcgatacgac taaaacgcca atggaattac ccagttggat cgaaccaagt
     8041 cgtaactttc cgagacatag cccaagcgcg aggaccacaa ataataacag aatgtaattc
     8101 ccatttaaca attcggcgac gtttatattc acggaggcta acttcttgtt tactagtaag
     8161 ctgttgaaag aaatggtaat ttacgataat gtgttttacc agaattcagg gtgcagattc
     8221 attcagcgca cctaaacgat agtaaagtaa caatatattt tactagtgta atcacattag
     8281 gtatcaacgg ctatatgaat tgcgttggcc tatattagca tggaatgcga agcggcttta
     8341 tcttactgaa cgccacactg gcgaaaaatg tgttcgatag acgcagtgtc aggaggaacg
     8401 agtgaaacat aaacaacgtt gggcgggggc aatctgctgt tttgtcctct tcattgtggt
     8461 gtgccttttt ctggcgacgc acatgaaagg cgcttttcgg gctgccgggc atcctgaaat
     8521 cggcttgctg tttttcattc ttcctggagc agttgccagt tttttttcac agcgtagaga
     8581 agtcctgaaa cctctatttg gcgcaatgct ggcggcaccc tgttcaatgc tcattatgcg
     8641 gctgtttttt tcaccgacgc gctcattctg gcaagagctg gcatggttac taagcgcggt
     8701 gttctggtgt gcgctggggg cactgtgttt cttatttatc agtagtttgt ttaaaccaca
     8761 gcacagaaaa aatcagtaaa gccctcaacg cgagggcttg tcagacgatc aggcgtccag
     8821 attttctttc acccatgcag caaaatcggt atagccgccg atatgttgct gatcgacaaa
     8881 aatctgcggc acggtttcta cgggtttacc tgccttttgt tgtagatctt ctttagtgat
     8941 cccttccgca cgaatatcta catactgata ctgaaaatca tcgcgttcat tgctcaattt
     9001 ctcagccaga tcttttgcac gcacacagta agggcaaccc gaacgaccaa aaataacggt
     9061 ttgcattatt tctctcctca tagatttatg cctgtaatga tcacgctaaa atgtattcgc
     9121 tgaaagtagg tttaacctgt tgcattaatt gctaaaagct ataactgtta aacacaatac
     9181 agtgaaaagt tttagactga aggctcactt tgcagaggga agcgtatgcg cgcgatcggt
     9241 aaattgccta aaggcgtgtt gatactggaa tttatcggaa tgatgctact ggcggtggcg
     9301 ctgctgtcgg taagcgactc cctgtcgctg cctgagccat tttctcggcc agaagtgcag
     9361 attctgatga tttttctcgg tgttttgctc atgcttcccg ctgcggtggt ggttattctt
     9421 caggtggtaa aacgtcttgc cccacagctg atgaaccgtc caccgcaata ttcacgttca
     9481 gaaagagaaa aagataatga cgccaaccat tgaacttatt tgtggccatc gctccattcg
     9541 ccatttcact gatgaaccca tttccgaagc gcagtgtgag gcgattatta acagcgcccg
     9601 tgcgacgtcc agttccagtt ttttgcagtg tagtagcatt attcgcatta ccgacaaagc
     9661 attacgtgaa gaactggtga cgctgaccgg cgggcaaaaa cacgtagcgc aagcggcgga
     9721 gttctgggtg ttctgtgccg actttaaccg ccatttacag atctgtccgg atgctcagct
     9781 cggcctggcg gaacaactgt tgctcggtgt cgttgatacg gcaatgatgg cgcagaatgc
     9841 attaaccgca gcggaatcgg ctgggattgg gcggggtata tatcggcggc ctgcgcaata
     9901 atattgaagc ggtgacgaaa ctgctgaaat taccgcagca tgttctgccg ctgtttgggc
     9961 tgtgccttgg ctggcctgcg gataatccgg atctcaagcc gcgtttaccg gcctccattt
    10021 tggtgcatga aaacagctat caaccgctgg ataaagacgc actggcgcag tatgacgagc
    10081 aactggcgga atattacctc acccgtggca gcaataatcg ctgggatacc tggagcgatc
    10141 atatccgccg aacaatcatt aaagaaagcc gcccatttat tctggattat ttgcacaaac
    10201 agggttgggc gacgcgctaa aaccgccacg tcgatgtatg atacgcgggc ttttgaccag
    10261 gtctgacaga gaggtgcagg gtgaaaattg ccatattgtc ccgggatgga acgctctatt
    10321 cgtgtaagcg gctgcgtgaa gccgctatac agcgcggtca cctggttgaa attcttgatc
    10381 cgctttcttg ctacatgaac ataaatcctg cggcgtcttc tattcactac aaaggccgca
    10441 agttacccca ttttgacgca gtgatcccgc gtattggcac cgccattacc ttttatggga
    10501 cggcggcact gcgccagttc gagatgctgg ggagctatcc gctcaatgag tcggtcgcca
    10561 ttgcccgggc gcgtgacaaa ttgcgttcca tgcaactgct ggcgcgtcag ggcatcgacc
    10621 tgcctgtcac gggcattgcg cattcgccgg atgataccag cgatttaatc gacatggtcg
    10681 gtggtgcgcc gctggtggtc aagttggttg aaggcacgca gggaattggc gtcgtgctgg
    10741 cggagactcg tcaggcggcg gaaagcgtga ttgacgcttt ccgcggtctg aacgcgcata
    10801 ttctggtgca ggaatatatc aaagaggcgc aagggtgcga tatccgctgt ctggttgttg
    10861 gtgatgaagt ggtcgctgcg attgaacggc gggcgaaaga gggctatttt cgttccaatt
    10921 tgcatcgtgg cggcgcggca agcgtcgcca gtatcacacc acaggagcgt gaaatcgcga
    10981 taaaagccgc gcgaacgatg gcgctggacg ttgctggtgt ggatattctg cgtgctaatc
    11041 gcgggccgtt ggtgatggaa gtgaatgcgt cgccggggct ggaaggaata gaaaaaacca
    11101 ccggtatcga tatcgcgggt aaaatgatcc gctggatcga acgccacgct acgacagaat
    11161 attgcctgaa aacgggtggt tagtcgcaat cacattactg atcatggttt tgcctgcgct
    11221 ttttgcgtaa gctgtgccgg tctttttatc gaaagaggtt gtacaaaatt atgacatcgc
    11281 tggtcgttcc tggtctggat acgctgcgtc aatggctcga tggcctgggg atgagttttt
    11341 ttgaatgtaa taactgtcag gctctgcatc tgccccatat gcagaatttc gacggtgtct
    11401 ttgatgccaa aatcgatctg atcgataaca cgatcctgtt ttctgccatg gcggaagtcc
    11461 gaccttcagc cgtattgccg ctggcggcgg atttatctgc catcaatgcc agttcgctga
    11521 ccgtgaaagc atttcttgat atgcaggatg ataatctgcc aaagctggtg gtttgccagt
    11581 ctttatccgt tatgcagggc gtaacctatg agcagtttgc atggttcgtg cgtcagagcg
    11641 aagagcagat ttcgatggtc attcttgaag ctaatgccca tcaactgctg ttaccgactg
    11701 acgatgaagg gcaaaacaac gttaccgaaa actattttct ccactgataa ttcctttcga
    11761 gcacgcagtc gctgccgcag tgactgcgcg ctgcaaaatt atctgctgtt tttaaccttt
    11821 tcttaaagat tatttcactt ctcttgtgtc gatttggctt tatcacatag agcaaatacg
    11881 cataaaaatt tgttaaatac cgttttttaa tccgagctat agtctcaaac cctggctaaa
    11941 gttattcttg cgatgcttct atatagcgag cagtgctggc cgggagagag ttctcttttc
    12001 ttacaccgcg ccgataaaaa aatatgcacg tttattgcat atctttcagt gtgacaactt
    12061 ttgttcgttt gttaacgaac tttcagaagg aaagagatgt gaccgcctta aataaaaaat
    12121 ggctatcggg tctggttgcg ggtgctctga tggccgtctc tgtcggcacg ctcgcggctg
    12181 aacaaaaaac actccacatt tataactggt ctgattatat cgcccctgac acggtggcca
    12241 attttgaaaa agaaaccggt attaaagtcg tctacgatgt tttcgactct aacgaagtac
    12301 tggaaggcaa attaatggcg ggtagcaccg gctttgactt ggtggttcca tctgccagct
    12361 ttcttgaacg ccagttgact gcgggagttt tccagccgct ggacaaaagc aaattgccgg
    12421 agtggaagaa tctcgacccg gagctgctga agctggtcgc caaacacgat cccgacaata
    12481 aatttgctat gccctatatg tgggcgacga ccgggattgg ctataacgtt gataaagtta
    12541 aagctgtgct gggcgaaaac gcgcccgtcg atagctggga cttgatcctc aaacctgaaa
    12601 atctggaaaa actgaaaagc tgcggtgtct ctttcctgga tgcgccagaa gaagtttttg
    12661 ctaccgtgtt gaattatctc ggcaaagatc ccaacagcac taaagcggat gattacaccg
    12721 gaccggcaac cgatctgctg ttaaagctgc gcccgaacat tcgttatttc cattcatctc
    12781 aatacattaa cgacctggca aacggcgata tttgcgtcgc tatcggctgg gcaggtgatg
    12841 tctggcaggc gtcaaaccgc gcgaaggaag cgaagaatgg agtgaatgtc tcgttctcga
    12901 ttccaaaaga aggggcgatg gcgttctttg atgtattcgc catgcctgcg gatgccaaaa
    12961 acaaagacga agcctatcag ttcctgaatt acctgctgcg cccggatgta gtagcgcata
    13021 tttccgacca tgtgttctat gtcaacgcca ataaagcagc cacgccgctg gtgagtgcgg
    13081 aagtccgtga gaacccgggt atttatccgc ctgcggatgt tcgtgcgaag ctgttcactc
    13141 tgaaagtgca ggacccgaaa atcgaccgtg tgcgcacccg cgcgtggacc aaagtgaaga
    13201 gcggaaaata atccgcagcc ttagatgccg ggcgggcgca ccacacccgc cggcaattcg
    13261 caccatcatg gtgcgcttgc acacattcaa tgccggagag cagccgtgaa tgacgctatc
    13321 cctcgcccgc aggcgaaaac ccgtaaggtg ctgacgccgc tattagaaat ccgcaacctc
    13381 accaaatcct acgatggtca acatgcggtg gatgatgtca gcctgaccat ctacaaaggt
    13441 gaaatcttcg cgctgctggg cgcatccggc tgtggcaagt ccacgctgct gcgtatgctg
    13501 gcaggtttcg aacaaccttc tgccggacag ataatgcttg atggcgtcga tttgtcacag
    13561 gttccgcctt acctgcgccc catcaatatg atgtttcagt cttacgcgct gtttccgcat
    13621 atgaccgtgg aacagaacat cgcttttggc ctgaaacagg acaaactacc gaaagcggaa
    13681 atttccagtc gggtcaatga gatgctcggc ctggtgcaca tgcaggagtt cgccaaacgt
    13741 aaaccgcatc agctttctgg tggtcagcga cagcgtgtgg ccctggcccg aagccttgcc
    13801 aagcgcccga aactattact gctcgacgag ccgatgggcg cgctggataa aaagctgcgt
    13861 gaccggatgc agcttgaagt ggtggatatt ctggagcgcg tcggtgtgac ttgtgtgatg
    13921 gtcacccacg atcaggaaga ggcgatgacc atggcggggc gcatcgccat tatgaatcgt
    13981 gggaaatttg tccagattgg cgaaccggaa gagatctacg agcatccgac tacccgctat
    14041 agcgccgaat ttattggctc ggtaaacgtc tttgaaggcg tactcaaaga gcgtcaggaa
    14101 gatggcctgg tgcttgattc gccggggctg gtgcatccac tgaaagtcga cgcggatgcc
    14161 tcggtggtcg ataacgtgcc ggtacatgtg gcgctgcgcc cggaaaaaat catgctttgc
    14221 gaagagccgc ccgccaatgg ttgtaacttc gcggtggggg aggtgataca cattgcctat
    14281 ctcggcgatc tttcggtgta tcacgttcgt ctgaaaagtg ggcagatgat tagcgcccag
    14341 ctacaaaacg cccatcgtca tcgtaaaggg ttaccgacct agggcgacga agtgcgtttg
    14401 tgctgggaag tggacagcag tgtggtgctg acggtttaag gagcaaagat gaatacactt
    14461 gaacctgctg cccagtcgaa accgccgggc ggatttaagc tgtggttgtc acagctgcaa
    14521 atgaagcatg ggcgcaaact ggtcattgcg ttgccatata tctggttgat cttgctgttt
    14581 ctgctgccat ttctgattgt ctttaaaata agcctggcgg agatggcgcg cgctattcca
    14641 ccttataccg aactgatgga gtgggctgac gggcaactgt ccatcactct taatctcggt
    14701 aattttctgc aactgaccga cgatccgctc tatttcgatg cttatctcca gtcgctacag
    14761 gtggctgcga tttcgacatt ttgctgttta ctgatcggct atccgctggc gtgggcggtg
    14821 gcgcacagta agccttcgac ccgtaatatt ttattactac tggtgatcct gccgtcgtgg
    14881 acctcgtttc tgatccgcgt ttatgcctgg atgggaatat taaaaaacaa cggtgtgctg
    14941 aataattttc tgctgtggct gggggttatc gatcaaccgc tgaccattct tcataccaat
    15001 ctggcagttt atattggcat tgtttacgcc tacgtgccgt ttatggtgct gccgatttat
    15061 accgcgttga ttcgtattga ttattcgctg gtggaagcag cgctggatct cggtgcacga
    15121 ccgctgaaaa cgttctttac tgtgatcgtg ccgctgacta aaggtgggat tattgccgga
    15181 tcgatgctgg tgtttatccc ggctgtgggc gagtttgtga tcccggaact gctcggtggc
    15241 ccggacagca tcatgatcgg gcgcgtgcta tggcaagagt ttttcaataa ccgcgactgg
    15301 ccggtggcct cggcggtagc gatcatcatg ttgctgctgc taattgtgcc gataatgtgg
    15361 ttccacaaac accagcaaaa aagcgtggga gaacacggat gaataattta ccggtagttc
    15421 gttcgccctg gcggattgtg attttgctgc tgggctttac ctttctctac gcgccaatgc
    15481 tgatgctggt tatctattcg tttaacagct cgaagctggt gacggtgtgg gccggctggt
    15541 caacgcgctg gtatggtgag ttattgcgcg atgacgcgat gatgagtgcg gttggttcaa
    15601 gcctgacaat tgctgcctgt gcggcaacgg cggcggcgat cctcgggact attgcggcgg
    15661 tggtgctggt gcgctttggc aggtttcgcg gatcaaatgg ctttgccttt atgatcaccg
    15721 cgccgctggt aatgccagat gtcatcacgg gcttgtcgct gttgttatta ttcgtcgcgc
    15781 ttgctcatgc cattggctgg cctgcggacc gcggtatgct taccatctgg ctggcgcatg
    15841 tcactttctg tacggcttat gtggcggtcg ttatttcgtc gcgtctgcgg gaactggatc
    15901 gctcgataga agaagccgcg atggatctcg gtgcgacgcc gctgaaagtg tttttcgtca
    15961 ttacgctacc gatgatcatg cccgcgatca tttctggctg gttactggct tttactttgt
    16021 cgcttgatga tctggtgatc gccagctttg tttccgggcc gggagccacc acgttaccga
    16081 tgctggtctt ttccagcgtg cggatggggg tgaatccgga aatcaacgcc ctggcaacat
    16141 tgatcctcgg cgcggtcgga attgtcggat ttatcgcctg gtatctgatg gcgcgcgcag
    16201 aaaaacagcg gatacgcgac atccaacgtg caagacgtgg ctgaagacac taaaatttgc
    16261 caacctggct acataatgct gcgcatgtcg cggcattgtt ttcatggaag acgaaacgtt
    16321 gggatttttt aagaaaacat cttcatctca tgctcgcctg aatgtgcctg cgctggtgca
    16381 ggtggcggcg ctcgccatta ttatgatccg tggcctcgac gtgctgatga ttttcaatac
    16441 gctgggcgtg cgcggtattg gcgagttcat tcatcgcagc gtacaaacct ggagtttaac
    16501 gctggtcttt ttaagcagtc tggtgctggt tttcattgag atctggtgtg cgttttcact
    16561 ggtgaaaggg cgtcgctggg cgcgctggct atatctgctg acacaaatca ccgccgcaag
    16621 ttacttgtgg gcggcttcgc tggggtacgg ttatccggag ctgttcagca ttcccggtga
    16681 atcaaaacgt gaaatcttcc atagcctgat gctgcagaag ctgccggata tgctcatcct
    16741 gatgctgctg ttcgttccct cgaccagtcg gcggttcttc cagttgcaat aatgtgtata
    16801 atcgtcgccc ctgatgatgt gaaggtcaat gtatgcagtg cgcactttac gacgcgggtc
    16861 gctgtcgttc ctgtcagtgg ataacgcagc cgattccaga gcaactctcc gctaaaaccg
    16921 ccgatcttaa aaatctgctc gccgattttc cggttgagga atggtgcgcg ccggtgtcag
    16981 gcccggaaca agggtttcgt aataaagcca aaatggtggt gagtggtagc gttgaaaaaa
    17041 cactgctcgg tatgctgcat cgcgatggca cgccggaaga cctttgtgac tgcccgcttt
    17101 atccagcctc atttgcgccc gtttttgcgg cgctaaaacc gtttatcgcc cgagcggggt
    17161 taacgcccta caacgtggcg cgtaagcgtg gcgaactgaa atacattctg ctgactgaaa
    17221 gccagagtga tggcggcatg atgctgcgct ttgtactgcg ttctgatacc aagctggcgc
    17281 aactgcgtaa ggcgctgccg tggttacacg aacaactacc gcagctgaaa gttattaccg
    17341 tcaatattca gccggtacat atggcgatta tggaagggga gacggagatc tacctgaccg
    17401 aacaacaggc actggcggag cgttttaatg acgtaccgct gtggatccgt ccgcaaagtt
    17461 tcttccagac taatccggcg gtcgccagcc agttgtacgc caccgcgcgc gactgggtac
    17521 gacagctgcc ggttaaacat atgtgggatc tgttctgcgg tgtggggggc tttggtttac
    17581 actgcgcgac gcctgacatg cagttaaccg ggatcgaaat tgcgccagag gccattgcct
    17641 gtgcaaagca gtcagccgct gaactgggct taactcgttt acaatttcag gcgctggact
    17701 ccactcagtt tgccactgct cagggggagg tgccggagct ggtgctggtt aacccgccgc
    17761 gccgcggcat tggtaaaccg ctgtgtgatt atctttcaac gatggcaccg cgttttatta
    17821 tctactccag ctgtaacgcc caaaccatgg cgaaagatat ccgcgaactg ccaggttacc
    17881 gcatcgaacg ggtacagctt tttgatatgt tcccgcacac cgcgcactat gaagtgttga
    17941 cgctgctggt gaagcaataa aaaagccaca ggtgcggctt cagattgctg atcaggtgcg
    18001 ctttgttcat gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc atgctaaatc
    18061 aatacattgc agagttgatg tgggccggat aagcgcagcg catcaggcaa tcttactttt
    18121 gtcatcagtc tcaattactg cggaaaccac tggtcattga ttttctgata tgtaccgtca
    18181 gctttaattg ctgccagcgc gttattcagt ttttccagca gagctttgtt atccggacgt
    18241 acagcgatac ccaggccggt gccgaagtat tgcggatcgg tcactttttc agtcgcaacg
    18301 cccagttgcg gattggtttt cagccattcg tttaccaccg cggtgtcacc aaatacccca
    18361 tcaatacgac catttttcag atcgataaag gcattctgat aactgtcata ggagacagtt
    18421 ttcacttccg ggtgctgatc ctgaatatat ttctggtgcg tcgtaccgtt ttccatccca
    18481 atgcgtttgc ctttcaggtc ggcaaacgtt ttgtaggtat cttttttggc aatcacgacg
    18541 gctgagtttt catagtaggg tgtggtaaac gaaacctgct tgctacgctc cggggtgata
    18601 tccataccgg agattacggc gtcatatttt ctgaatttca gtgacgggat caggctgtcg
    18661 aacgcgtgat tagtaaaagt acattctgcc tgcatttgtt tgcacaaggc ttttgccaga
    18721 tcgatatcaa agccgacaat ctcattatta gcacctatag attcaaaggg tggataggtg
    18781 gctgaaacgc caaaattgat tttctctgcg gcagaagcac cgaaagtaaa ggaagcaagt
    18841 aaagcggcaa gaactaactt tttcatgatg gaactcccgt ctgtcaatct tatgattttt
    18901 ggccgtgtct gcggcatggg ataacaatgc caccaagtga atttatatgc aataaacatg
    18961 attaaataat ttaaatgaaa taaaaaaaga cggataactt agttagttgt ccgtcttcat
    19021 tataagaatt tatgcactat gtaggccgga taaggcgtcc acgccgcatc cggcacaggc
    19081 accgtgctga tgtctgatgc gacgctggcg cgtcttatca gacctacaaa tccccccggc
    19141 gaatgtacgc agctacatta attccgccgt tcgaatgcca gtgctttgcg ctcgatcaga
    19201 cgcatcatca gcgtcagcag gccgttgacg accaggtaaa taatccctgc cgcaccgaac
    19261 accattacat cgtaggtgcg tccgtacaac aactggctgt atcccatcac ttccatcagc
    19321 gtaatggtgt aagccagaga ggtacttttg aacaccagca ccacttcgtt ggaataagaa
    19381 gagagcgagc gtttaaaggc atacggcagc aggatcgcca gcgtatcttt tttgctcatt
    19441 cccagggcgc tacaggactg ccactgacct tctgggatcg cacgaattgc accgtaaaac
    19501 agctgcgtgg tatacgccgc actgttcagc gataacgcaa tcagcgcaca taaccacggt
    19561 tctgacaaca aatgccacag tgccggatac tcctgcaacg tcggaaactg gcccggcccg
    19621 taataaatca ggaagatctg caccagcagc ggcgtaccgg taaacagcgt gatataaccc
    19681 cgcaccagcc acaccagcac cggcgttttc agcgtcagga tgatggtaaa aatcaatgcc
    19741 agaatcagtg ccacaatcag cgaggcaacg gttagcgtca ggctggtgtg cagccctttc
    19801 atcagctcgg gtaaatactc aaacattagc tgggcctccg ctcaaaacgt gtcgcgcgca
    19861 ggtcaatgcg tttgagaatg tactgactga gcagggtgat caccaggtaa atcgccgccg
    19921 ccacaatgta ccaggtaaat ggttcctggg tacgagtagc aatgcttttg gtttgtaaca
    19981 ttaaatcatt cacactaatc aaactgacca gcgcggtatc tttcagcagc accagccact
    20041 ggttaccgag gccaggcagc gcatgacgcc acatctgcgg catcaccaga cggaaaaaga
    20101 tagccgattt cgacagcccc agcgcctgac cagattccca ctggcccacc ggtacagctt
    20161 tcaacgcgcc gcgcagcgtt tgcgaggcat aggcggcata cagcagtgac agagcgatga
    20221 caccacaaag gaacggactc acgtcgaagt tctcaatgtc catctgcacc gggatctgca
    20281 cgaacccaag attgatagtg aagccatccg aaagcgtcag cagtaactgc gaggagccaa
    20341 aatagataaa cagcactacc agaatttctg gcaggccacg cagaatggtt accagcgctg
    20401 aacctgccca cgcgacagga cgccatttcg ctgactccca taccgcaaag aacatcgcca
    20461 gcgccagccc gacaatcaat gcacaaacgg caaggccgac ggtcatcccg gcggcgcttg
    20521 ctaaaggaaa aaattcattc atcaggaatt acttctggaa ccatttgttg tagatggttt
    20581 cgtaagtgcc atctttcttc actttttcca gcgcagtgtt gagtttctgc tgcagttcag
    20641 tgttgccctg acgtaccgcg atgccgaggc cagtaccgaa ataatcttta tcggtcactt
    20701 tatcgcccac tgccgccagt tttgggttat ctttcagcca ctcagtgacc actgcggtgt
    20761 caccaaatac gctgtcgata cgcccgtttt gcagatccag ttttgcgttc tggtagctgt
    20821 catacggaac ggtggtgatt tccgggtgct tatccataat gaacttctgg tgtgtcgtcc
    20881 cgttctgaac gccgactttt ttgcctttca gctgatcaac actggtgtat ttgccttgct
    20941 gacccacaaa cagggcagag ttgtcatagt acggggtagt aaacagtacc tgcttttcac
    21001 gctccggagt gatatccatg cccgccatca ctgcttctac gcgacggaat ttcaggcttg
    21061 ggatcaggct gtcaaacgcc tggttagaga aagtacaggt tgcatcaatc tctttacaca
    21121 gtgcctgtgc caggtcgacg tcaaaaccaa cgatctggtt gtttgcatca atcgattcaa
    21181 acggaggata ggaggcttcg gtagcaaaac gaatggtttc ggcagctgtg gcggaaagac
    21241 taaaacctgc aattaacgcg gcaatcagaa cttttttcat tgttgttatc ccgaatctta
    21301 gtgagagaga tagtttttaa atgcttcggt ttgcggctcg gtaaagcagc tcgcgtcgcc
    21361 ttgttctacg atatgaccat tttccatata caccactcgg ctggcggttt tacgcgccac
    21421 ttcaacttcg tgggtgacga tcacctgggt aatattcgtt tctgccagct cacgaatgat
    21481 gctgacgatt tgtgccgtaa tttccgggtc cagtgcggcg gtcggttcat cgaacagcag
    21541 tacctgcggt tccatcatca acgcacgggc aatagcaaca cgctgctgct gaccaccaga
    21601 aagatgcagc gggtaacgat cgctataagg tttgagacgc agacgttcca gcagtttttc
    21661 tgcacgggcc agcgcctgat ctttactcaa ccccagtaca cggcagggcg cttcaatcag
    21721 gttttgctgc acggtcagat gcggccacag gttgtattgc tgaaacacca tgccaacgtt
    21781 acgacgcaaa tcgcgaatcg ctttgtcaga gggtgttttg gtgaaatcga aatggttgcc
    21841 tgcaatgttg agcgtaccgg agcgcggcat ctcaagcaga ttgagtacac gcagcagcga
    21901 gcttttaccc gcgccgctgg ggccaagtaa caccagcgtt tcgccctgtg ggcaatccag
    21961 cgtgatatcg aacagcgcct gatgcgcgcc gtagaagcaa ttaatgccgt ttaattgaat
    22021 actcattgac actcgtatac tggcagtctg atagctattg aggtcgaaga tagtaccttt
    22081 gacagaataa ttatgcaata tttatgcttt aaaagttaaa agcaaagcgc attattcaat
    22141 aaacatagca caaaataacg ggggcggtgg tcggtgagca taaatgtcgg cattcctcac
    22201 gaaatgccgg ataatttacg tggtttattg gttgatcaag gcgttagcga ttctcgatgg
    22261 actgacggag cgtacccgcc gtggcatgaa cgctaccgcc taagtagcgc acatcgtcga
    22321 tgacccaaca ctggccttcc tgaatcatta acacttcatc ctgccagccc tggtcaccct
    22381 gtttgagatc cacccgcaat ggaatgttac gggcatcacg attagggata gtcgatgcac
    22441 tggcaacgtg ggcgctgtct ggcaaggtgg ttcgactgga gaacggatcg ctgcttaaca
    22501 attcacgatg actattatcg cggctggcat cgctaagttg ggttgccagt ttgtcactca
    22561 aataggggcg cagggcggta atgtcattgc tgcgatgttg aatgcggtag tcgtagaatt
    22621 gttgagccac actgtcaggc cctccctgga cacaggaacc ggtgcggggg ccgttatctt
    22681 tataagctgg cgtgacggtg gtacaggcac tcagcagcag tgcacatgga ataagtagag
    22741 atagtgaacg gtagcgcata atcatttcct tattagcttg ctgtctgcgt cacactacga
    22801 ccaaacgcta tcagattatt taatacattg tttttaatac aattctgaaa caatctgaac
    22861 acctgattgg gtttgagtcc attgctggct acccactcaa ggttgctcgt agctgactta
    22921 cttaaattac ccaaacaata gacagtgtaa accatttgtg aggttgagcg tgttatatgg
    22981 ctgaaatgat catttaaggt aaatatgata gttctgaaat ggtggtataa atcctcctgt
    23041 agaggttaag agcatctgca agaagccagt ttcacattta taacgcgtga taaatgaatg
    23101 cggttattgt ctaatcatta caaaattttt cacatgcgat aagagatcct gcgattaaga
    23161 tgattgccaa aattacgttg tatttttgaa aatgagcgaa atttgatagt cccagctttg
    23221 actctcgata atattattta tgctacaatt gtgggtaaag aatttaagct aatgaatttg
    23281 agtgaagaat aaattaaatt gcttcagtat ttcgttaatt aatatgaata caaaaggtgt
    23341 tgtgctttta taattataaa gcgcgaggag ttcggtggat tatcgttttt cttggatgat
    23401 ttttaatatg ataaacaata tatctgatca agcatcatca tttccaggca cacaactaaa
    23461 tcaaagtgat aatttcctgg attcactgag ggaatttttc gcgatattaa attccagtcg
    23521 taagggtgag ttgtctacat gggataccat ctacttgcat ctgatattag caataaatgc
    23581 tgacagtgat ctcataaaaa atgatgtgtt gctcgcggaa aatattccat cagcgaatta
    23641 ccaatttaat acatttttta gcaatgcttt cgagattgat gttaaaaaat atctgggtaa
    23701 atcagaagat aacgaagttg aaataaaagc gggtaatgaa agaatctcta ttggaatacg
    23761 taatgtatcg aatggaaaac ttgaaagaca acagtttctt ttcccactgg actatgagaa
    23821 taaattacaa gaacaattag ataagtattt tacgattgaa tcgcacccat tattatacag
    23881 gtataccatt ggtagtaaga ttgcgaatgt gatttttgaa aagttataca gtcgtattga
    23941 ctttaacaaa gaacaatata tatcctttat aaaggacgca tttattcatt tttatgatta
    24001 tagcaggcga tatgctatta gtgagaatat cgataaagac gctgtaacaa acaatatcgc
    24061 attgatgagc actttttatg acagtgataa tacgtcaggt gaggttttaa ataatgattt
    24121 tacagaagaa gagtcatttg aaactgcgtt agatgtagag catgcaatag tgcttggatt
    24181 tgctgatgat aattttgaaa caaaacctgt tcattatcag gatttattaa cacgatttag
    24241 tgcctttcaa gataccgtgt ttaatctgtt tcctgaaatg cactcttcgc attaccacga
    24301 tatttgtagc gtaagtgtgg atatgacaaa gggaacgcaa tgcatgattc acttgatggt
    24361 gaatgaagaa gttttcatgt ctctgcctgt tccagttgcc acgatggtac gtgaagatgc
    24421 aagtaattta gtcaatctta aaacactact caatgatggt tgtttcatta aatatagtca
    24481 ttttaatgac gtggcattaa taaagcaaaa cataagtaat ctctatttat cacacactgt
    24541 tgtgaatgaa agcatattga aaaaatgttg ttttgaaaac ggcagtttag gtgatgttaa
    24601 aataacaaat agcaatgtaa taaacagtgc attcaaaaat atttcgttta gatctgttaa
    24661 aattaataac gtaaatactc attctttaaa atttattaac tgcaattttt tcaatgttga
    24721 tatgataaga gttaatctca gcaaatgtct ttttcatgaa tgtagtatgc atggtgtcaa
    24781 gataaaaccc tggttgcctg taaaatggac aaaagaatta ataagtgatt atttgtatgg
    24841 ctgtttacta tctctgtact ctatctgtgc aagggatata tataatatga acgccgggaa
    24901 taacgtgaag gttgctgcgg atgctttcct tgaaattatt ttctctctta aaaataaata
    24961 ttgtattaaa ctattaagcg ctcaggatcg ggcgtttata tacgagtttg caagaatgat
    25021 cttcgcttat attaatgaca aaagcattga gatcttatta ctttcttgtt ttgcggcggc
    25081 tgaccaaaaa gcaatccagc ggtatagacc tcagtcacaa gatggggaag actttaggag
    25141 tcatttacaa tataagttgc ctctttccgc tcattaatgt tgttggtgca acataataag
    25201 acaggtaagc ggtaccctat aatgaaacca atatcgggtt atgcaacgga taatcgtaaa
    25261 gacttaagtg ggtgtttttg acacaatgag atgatttcct aaactgatca aaaccttctt
    25321 cccaacatgg catatcagtc cgataatgct ttagagatcg aaggcttaac aaacaaggag
    25381 atcgatcatg caattttcta caaccccaac tctggaaggc cagaccatcg ttgaatattg
    25441 cggtgtggtg accggcgaag cgattttagg tgccaatatt ttccgtgatt tctttgccgg
    25501 tatccgcgat atcgttggcg gacgttccgg tgcgtatgaa aaagaactgc gtaaagcacg
    25561 ggagatcgcc tttgaggaat taggctccca ggcgcgggcg ctgggggccg atgccgtagt
    25621 tggtattgat atcgactacg aaacggtcgg gcaaaacggc agtatgctga tggttagcgt
    25681 cagcggtacg gcggtgaaaa cgcgtcgatg agaagagtct tctggctggt cgctgccgct
    25741 ctgttactgg cagggtgtgc aggcgaaaaa ggcattgtcg agaaagaggg atatcagctt
    25801 gatacccgac accaggcgca ggcggcgtat ccgcgcatta aagtgctggt gatccactac
    25861 accgcagatg attttgatag ctcgctggcg acactgactg ataagcaggt cagctcgcat
    25921 tatctgatcc ctgcggtgcc accgcgatac aacggtaaac cgcgcatctg gcaactggtg
    25981 ccggaacaag aactggcctg gcatgcgggg attagcgcct ggcgcggggc aacgcgcctt
    26041 aacgacacct ctattggcat tgagctggaa aaccgtggct ggcaaaaatc atccggagtg
    26101 aaatattttg ccccgtttga acaggcacag attcaggcgc ttattccgct ggcgaaagat
    26161 attattgccc gttatcacat caagccggaa aacgtagtgg cacatgcgga tatcgcaccg
    26221 cagcgcaaag acgatccggg gccattattt ccctggcagc aactggcgca gcaggggatt
    26281 ggtgcctggc cggatgcgca gcgggtgaac ttttaccttg ccgggcgcgc gccgcacaca
    26341 ccggtagata ccgcgtcatt gctggagctt ttggcgcgct acggttatga cgtaaaacct
    26401 gatatgacac cgcgcgagca gcggcgcgtg attatggcat tccagatgca tttccgcccg
    26461 acgttatata acggcgaagc ggatgcagaa actcaggcga ttgccgaagc attgctggag
    26521 aaatacgggc aggattagcg cggcagtttt ccgtggtcgc gtagccaggc ggcagttttc
    26581 tcgatgcctt catccagggt gatgaccggc tgataaccta actcttcctg cgcacgcgta
    26641 atatccagcg taaagtcaaa attcaacttg gagacgccgt agtgggtcag cggcggctct
    26701 tttgctgact tgcggcctaa acgctccatg ctgtgggcga tcatatccag catcgggtaa
    26761 gggacggaac gaatacgaca gtcaatattc aactcgtcga tcagcttctg cacgatgctg
    26821 cgcagtgtgc gatgctcgcc gttggtgatg ttgtacacac gcccggaagg tagcttatcg
    26881 caggcttcct ggcttgccag ccacattgcg tgcacggcat tttcatagta ggtcatatcg
    26941 accagcgcac tgccgccatg cggtaacaga atactgccgt agtggtgcat catatgcgcc
    27001 agacggggaa taaagacttt atcgtgcggt ccgaacagac tttgtgggcg cagaatagta
    27061 aagcgcgttt gtggattcgc ctgcgaaagc atattgatca cttcttcgct ggctgctttg
    27121 ctgcgggcaa actcgttggc gaagcggtga gggcgaaaat cttctttaat atcgcgatgg
    27181 tggtgataat cgaagtacag ggagggggaa gagatatgaa taaagttacg cacaccccag
    27241 gcgacagccc attcacccag gcggcgagtg gcgcgaacgt tagccagatc gaaagcctgt
    27301 tgtgtccccc agggtgaggt aaagctggag cagtgccaca gcgtatcaat gcccgcgagc
    27361 atcactttag cttgtgatga aaccagctcg gtcagatccg tcagaacaaa ctctgcgccc
    27421 attttttcca gcaatttacc cattgcttcg ttgcgaccgc tcgcacgcac gctgatgcct
    27481 ttctggcata aaaactctac cgcgtttcga cctaagccgc tggtggcgcc ggtaaccagt
    27541 accttcatat caatccactg ttgttgagaa aatgacgtgc gcattcttcc gtgatttccc
    27601 ccatgatgca atgggaaaca tgaaagaata acgcaggttt tgtcgattaa tctgtgcttt
    27661 gttctgccag tctggcgatt tgttttgcca ttccgcgaaa aataaacaga tgcgcgggga
    27721 tcatcaataa ccagtaaaac agccccggca taccgtgcgg atgccagaaa gcgcggacat
    27781 cgatagtacg atagtcacct ttatcttcca gggtaaaaca cagtcgtccc agccccggcg
    27841 ctttcatgcc aaataacaac gtaagttgct ttttcggttc aacgacaatc actttccagc
    27901 tatccaccgc atcgccggtc tgtaaatatt cgcgctccgg gcggcctttc gccagattat
    27961 gaccgatcgc gcggtccatc aacgcccgtg tctgccacaa aatattgcca aagaaataac
    28021 gctctttacc gccgatttgg ttcaccacca gccataaagc cgcaaggctg gcggacgttt
    28081 taacggtaaa ccccgcctgt ttggcgaaat aaccgtactc cggtcgccag cgggcaaagg
    28141 cctgagcgtc gtagccccag tcgctggagt tgaccagttt ttcttcctct ttcaacgtgc
    28201 tacgtaccgc gtcatcgaaa gcgatcagcc gttgtgggat gagtgcacgt agcgcggtat
    28261 catctgccag cagatcgtgt ttcagcccct gaatcaacgc cctggcggtg gtgggcggta
    28321 cggaagtaat cacattgaga aaccacaccg aaatccagcg ggtggggagg gggatgggga
    28381 tcaaccagcg gcgcttaccg ctcaccgcca taaaatgttc aaactgttgc tgataactga
    28441 gcacctctgg tccggcggct tcgaagatgc ggtgtttgtt ggctggatgg tctaacagcg
    28501 ccaccagata gtgcagcaag ttttccagcg cgatgggcgt ggtgcgtgaa cgtacccagc
    28561 gtggcggcgt taacactggc aggttgtaga ccatatcgcg catgacttcg aacgccgctg
    28621 aacctgcgcc aacgataatt ccggcccgaa gttcggtcac tggtacattc gcttcacgaa
    28681 gaatgtccgc cgtagcctga cgagctcgca gatgatccga ctgctcatgt ggcggggcct
    28741 gcaacgaact gagaaagatt aattgcttaa ctggtacttc acgtagcgca tcgcggacgt
    28801 tgagagccac ctggcgctcc tgagcgataa aatcgccgcc ttcgcccatg ctgtgcacca
    28861 gaaaatagac ggtatcgata tcctgcaaca gggcaggaag gttatccggc cagctgagat
    28921 cgactttatg gcaactgacg tttgccagtt gcagctttgc aagcctgtcg acatgacgtg
    28981 ccgccgccag gatctgatgc ccttgctggc tgagtgtgcg caccagatgc tgaccaatgt
    29041 agccactggc accgagaact aaaatgcgtt gcggcacgtc tctctcctta acgcgccagg
    29101 aatgcgcgcc agtgggcggc gacttccgcc agttgttcgc gcgagacgtc aagatgcgtc
    29161 accaggcgga caatcggcga ggcgttaatc agcacgtttc tcgctttcat gtattcgcct
    29221 aacgcggcag cattttcttc cccgacgcga acaaacagca tattagtgtc ctgacgcatc
    29281 acatccgcgc ctgcttcacg cagttgctcc gccatccagg cggcgttgtc gtgatcttcc
    29341 tgcaaccgtg cgacgttatt tttcagggca tacatcccgg cggcagccag aatcccggac
    29401 tgacgcatcc cgccaccggc cattttccgc cagcggatgg cacgtttaat gtaatcacga
    29461 ttaccgacga gtaatgaacc gactggcgtc ccaagacctt tcgacaggca aatggtgaac
    29521 gaatcacaat attgcgtgat ctctttcagt tcgcagccgt aagccaccac ggcattaaag
    29581 atgtgcgcac cgtcaacatg cagcgccaga ttgcgctcgc gggtaaattc ccatgcttct
    29641 ttcaggtatt cacgcggcag cactttgccg ttgtgggtgt tttccagact gagtaatttg
    29701 gtgcgggcga aatggatatc gtcgggtttg attttcatcg ccactttatc cagcggtagc
    29761 gtgccgtcgg cagccgcgtc gatgggttgc ggctgaatac tgcccagcac cgctgcgcca
    29821 ccggcttcaa acagatagtt atgcgcggcc tgaccgacaa tatactcttc gccacgttcg
    29881 cagtgactga gcagagcgac caggttggcc tgagtgccgg taggcagaaa aatggcggct
    29941 tctttaccgg aaagctctgc tgcatagtcc tgcagagcat taacggtagg gtcgtctccg
    30001 taaacgtcgt ccccaaccgg ggcggccatc atcgcttcga gcatggcgcg gctcggtcgg
    30061 gtaacggtat cactgcgtaa atcaatcatg gcatgtcctt attatgacgg gaaatgccac
    30121 cctttttacc ttagccagtt cgttttcgcc agttcgatca cttcatcgcc gcgtccgctg
    30181 ataattgcgc gcaacatata caggctaaaa cctttggcct gttcgagttt gatctgcggt
    30241 ggaatggcta attcttcttt ggcgaccacc acatccacca acaccggacc gtcgatagag
    30301 aaggcgcgtt gcagggcttc atcaacttca gaggcttttt ctacacggat acccgtaatg
    30361 ccgcacgctt cggcaatgcg ggcaaagttg gtgtcgtgca gctcagtacc gtctgtcagg
    30421 taacctccgg ccttcatctc catcgccaca aagcccagca cgctgttatt aaagacgata
    30481 attttcaccg gcagtttcat ctgcactact gagaggaaat cgcccatcaa catgctaaaa
    30541 ccgccatcgc cgcacatggc gaccacctga cgttccggct cggttgcctg cgcacccaac
    30601 gcctgcggca tggcgttagc catcgaaccg tggttaaacg aacctaacag gcgacgcttg
    30661 ccgttcattt tcagataacg tgccgcccat accgttggcg tgccgacgtc acaggtaaag
    30721 atggcgtcat cggcggcaaa atgactaatt tgctgcgcca gatattgcgg gtgaatggct
    30781 ttctcgctcg gtttagctaa atcgtccagc cctttgcggg catcgcggta atcttccagc
    30841 gctttatcca gaaacttgcg atcggttttt tcttccacca gtggcagcaa tgcgcgcagg
    30901 gttgatttga tatcgccgac cagcgccata tctaccttgc tatgtgcgcc gatgctggct
    30961 ggattaatat caatctgaat aattttggca tcggtcgggt agaacgcacg gtaggggaac
    31021 tgagtaccga gtagcactaa cgtgtcggcg ttcatcatgg tatggaaacc tgacgagaat
    31081 ccgattaacc cggtcattcc aacatcatac ggattatcgt attcgacatg ctctttaccg
    31141 cgtagggcat gaacaatagg tgctttaatt ttcccggcaa actcaactaa ctctttatgc
    31201 gcccccgcgc agccgctgcc acacatcagg gcgatattgc tggaataacg cagcagttgc
    31261 gccagtttgc gtaactcttc ttcttccggc gtcacgactg gctgtggcgc atgataccag
    31321 tgcgtggttg ccccttctgg cgcaggtttt aacgccacgt cgcccggtaa cacaacaacg
    31381 gaaacgccac ggttaagcac cgctttgcgc atagcaattg ccagcacttg tgggatctgc
    31441 tccgggctgg aaaccagctc gcaatagtga ctacattcgc ggaatagctc ttgtgggtgg
    31501 gtttcctgga aatagccgct gccaatttcg ctggagggaa tatgagcggc aatcgccagt
    31561 accggaacgt tattgcggtg gcaatcgaat aggccgttga tcaagtgcag gttgcctggg
    31621 ccgcacgatc cggcacagac cgccagttct cctgaaagtt gtgcttcagc gccagcggca
    31681 aacgccgcca cttcttcatg gcgggtggac atccactcga tggtgcccat gcgattaaga
    31741 ctgtcgctaa gaccgttcag agagtcgccc gtgactcccc agatgcgttt cacgcctgcc
    31801 gattcgagtg ttttggcgat ataagctgca accgtttgtt tcatggttct ccatctcctg
    31861 aatgtgataa cggtaacaag tttagttcat ctgacggagg gggaagggat gggagagaaa
    31921 ggaggcacta acggttaaat agcccgatga aaggaatatc atcgggcata aggcgattat
    31981 gcgagaacca aatccccctg cggatggcag gagcaggcca gtacgtaacc ttcagcgatt
    32041 tcggcgtcgg tcagcgtcat tgtactgctc accgtatatt caccggaaac cacttttgtc
    32101 ttacagcagc cgcaaacacc cgcacggcag gcagcgacaa ccggaacgtt attgctttcc
    32161 agcgcctcca gtagcgtggt gccaaccggg gcgtaaaatt ctcgtgccgg ttgcagtttg
    32221 gtgaatttca gaccgctggt cgccgcttct gctactgggg tgaagaattt ctctttaaag
    32281 aaacgcgtca cgccgagcgc tttcacttcc tgctctaccc aatccatata cggagccggg
    32341 ccgcaggtca tcacggtacg tgaagctaag tcaggtacat ctgccagcag ttcgcgagtg
    32401 agacgaccag cgataaagcc ttcggtaacg ttattttctg ccaccagcgt taccggatag
    32461 ttacgccact catcggcgaa aataacatcc tgcggcgtac gcacgttgta gatcacccgc
    32521 acatcggcct gtggacggtt cttcgcaagc cagcgacgca tcgacataat cggcgtgacg
    32581 ccgcagcctg ccgccagcaa caggaattta tcttctgctt tatcgtcgca ggtaaattcc
    32641 cccatcgcgt ccgaaagcca gagataatca ccgcgtttta catcgcgcgt cagccactgg
    32701 gagccgacac cgtcatcaat ccgccgcacg gtcagggtga tatattcact cacgcctggc
    32761 gtggaggaaa tggtgtaagc acgcagcgtt tccgctgagt ttcgcacgct gaccagtgca
    32821 tattgcccgg cgcgatatgg gtagtgatcg tggcaaatca gggaaatcgt ccacacatcc
    32881 ggcgtttctt gcgtaatgtg atgaacctgc atccgccacg ggcattgatt cgttggcatc
    32941 gtcatcgaca aactccttac gcgctcaaca gttgcttcat gtcttcttca acagtggtga
    33001 tagaacgcag gccgaatttc tcgttcagca ccgccagcag gtctggtgtc aggaaactag
    33061 gtgcagtcgg gccggtgacg atatttttca cgcccagaga aagcagcgtc agcagaatga
    33121 caatcgcttt ctgttcaaac caggagagca ccagcgacag cggcagatcg ttcacaccac
    33181 agcccagttt ctctgccaga gtgacagcca gaataattgc tgagtaagca tcgttacatt
    33241 gacctgcatc taccagacgc ggcagacctt cgatatcgcc aaactcaagt ttgttaaagc
    33301 gatatttacc acaggcgagg gtcaggatca ggcagtcatc cggcacgctg gtggcgaaat
    33361 cggtgaagta gtggcgctcg ccgcgtgcgc cgtcacagcc accaagcagg aagatatgac
    33421 gcagtttttc acggctaacc agatcaatca gcgtatcggc tgcaccaagc agcgtctggc
    33481 gaccaaagcc cacggtgatc aggtgaggaa tttcgctgta cgggaagccc gccatttgtt
    33541 gcgcctgggc gatgaccgca gagaaatctt cgccatccag atgacgcacg ccaggccagc
    33601 caacgatgct gcgggtccag atacgatcgt cataagcgcc tacggttggg tcgatgatgc
    33661 agttcgaggt catcacgatg gggccaggga aacgagcgaa ctccacttgc tgattctgcc
    33721 agccgctgcc gtagttaccg accagatgct tgaatttacg cagctccggg tagccatgcg
    33781 caggcagcat ttcgccgtgg gtgtagacat taacgcccgt gccttcggtc tgttccagca
    33841 ggttgtagag atctttgaga tcgtgaccgg aaatcagaat gcatttaccc gccgtcgctt
    33901 tgacgttgac ctgggttggc gtcgggtgac cgtatttacc ggtttcgcct gcatccagaa
    33961 tgctcatcac tttgaagttc atctggccga tttccattga acactcaaga agcgcgttca
    34021 tatcggcagg ccaggtcccc agccacgcca tgattttatg gtactgggca taaatatcgt
    34081 tgtcgtattg accgagaaca tgcgcgtgtt ccatataggc cgccgcacct ttcaggccat
    34141 acaggcacag cagacgcagg ccgagaatgt tttcgccaat cgccgcttta tctttgttag
    34201 gggtaaattc tgctgcctga cgttgcagct cgccgagatc atcgctcacc agttgcaggt
    34261 cagccatcgg gttatcgacg cgcgcgttgg catctacagc caggcattgt gctttcagcg
    34321 cctcgcgcaa ggcaatcgct tcacgagcgt agccgacaat acgcggagaa tcgaagttaa
    34381 cgttggtcag ggttgagaaa aaggcacgtg gcgcgaagct gtcaacatcg tggttgatga
    34441 tgccgtattc acgcgctttt accgcccagg cagaaagccc ttgcagcgcc gcgatgagta
    34501 aatcctgaag gtcagaagtt tccgccgttt taccacacat cccctgcgcg tatgagcagc
    34561 cgtttcctgc cggagtacgg atagtttgtt cacattgcac acaaaacatg atcacacctt
    34621 ttaaagttat atttaatata catgtttaag gttaagacgc ttaacgcggg gataaaaggg
    34681 atttttcatg caactttaag ggagattgat ttagcgcaat tttggcggca gggatctacc
    34741 gccagagagg tattacgcag agaaaaaggc gatgaggatc ggcactaaca ggctaagaat
    34801 aaaaccgtga acaattgccg ccgggaccat atccagcccg ccagtacgtt gaagaacggg
    34861 cagggtgaaa tccattgatg tggcaccgca taagcccagt gcagtagagc ggctgcggcg
    34921 aatcagccca gggatcaaca taatagcaat cagttcacgg gccagatcat taaaaaacgc
    34981 cgcgctcccg attaccggac caaaagattc ggtcaataaa ataccggaaa gagaatacca
    35041 gccgaaaccg gaggccattg ccagcgcggt attgatgggg agatcaagaa taaaggcgtt
    35101 aattaaacca ccaattaatg aactgacaac caccaccacg gcgacaatca ttccccggcg
    35161 attaaggaca atctgcttta aggtcatgcc attattgcgc aactgaatac caacgaggaa
    35221 aagtagcaaa attaacgtgt attcactggc ttcggtcgcg tgttgtaaga aagccagtcc
    35281 actcagacca atggcaaaac caatcactac tacgccgcac agttttagcg actccagcgc
    35341 catcgcaata cgcgacggga gtttttcttg ctgatggtgg ttgcgccacg gcaggcctcg
    35401 ctccagccac atcagggcgg caatattaca cagtaaaata acggtaatac tgacggcaga
    35461 ataatgcaga atcgccaaca ggttactggc gaggttatcg agaaacgcca gactgatacc
    35521 cataaaaaag agaataaggt aaaccatcca gcttaatagc tgattaataa cttttaacgc
    35581 agcttgttgg cgaagcggaa tgaggtaacc cacaatcagg ggaaccagaa tgattaacag
    35641 cccagaaaac atgaaaatcc agtccttgca aagaagaagt cgaaatgcgc gatgacacac
    35701 tactgaaagc ggaaggacga gtaaagttgc aattaaaagg aaatgttatg catcagaggg
    35761 ggatttttaa tcctatctga gtgtttagca ttgttgcgct cctttcatgt gcaatgccat
    35821 ataaagccac tggcggaggg agcgcgcagg gggcagccag tcgccgccgc cccctgcacc
    35881 cccgggctct ggcgaacaaa atcgccgctg cgcggtacct tcagcttatc cccttcggca
    35941 accaggtcgg gcgcgaggta acgtcctgta agacgcgccc tcagcccaca tccatgtggg
    36001 ctgccccggc cttcggggaa cgcttcagcg attttgacgc caccaatacc cgtgcggttt
    36061 gtaatgtcga agataaaaca accagggcag ataaaatgcc aatgctaatt aatcacgctt
    36121 ttccagcaga gtccggtaaa tcagaccacc gataatgccg ccgacaattg gcaccaccca
    36181 gaagaaccac agttgttcta atgcccagcc gccctggaag atagcaaccg cggtgctgcg
    36241 cgccgggtta acagaagtgt tagtcaccgg aatactgatt aagtgaatca gggttaaggc
    36301 cagaccaata gcgatcggcg caaaacctgc cggagcgaat ttgtcggttg caccgtggat
    36361 cactaacagg aagcccgcac tcaataccag ttcaactacc agcgcggaaa gcatggaata
    36421 accgcctggt gaatgctcgc cataaccgtt agaagcaaaa ccgctggctg ccgcgtcaaa
    36481 acccgtttta ccactgacaa ttaaatacag cagcgccgct gcaacaatac cgccgacaac
    36541 ctgggcaatc acgtagccaa cgacttcttt tgccggaaaa cgtccgccag cccataaacc
    36601 aatagtgacc gccgggttaa aatgaccacc agaaatatga ccaacagcga aggccatcgt
    36661 cagaacggtc aaaccaaatg ccagcgccac gccggcaaaa ccaatgccta attccgggaa
    36721 gcctgcggcc agtacagcac taccacagcc accaaaaaca agccagaaag taccaaaaca
    36781 ttcagctgct aattttctga acatatccac ctcaattaaa aattgaccct gtgaaaaata
    36841 gggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
    36901 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
    36961 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
    37021 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
    37081 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
    37141 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg ccagcgtaaa
    37201 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
    37261 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
    37321 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
    37381 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
    37441 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
    37501 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
    37561 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
    37621 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
    37681 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
    37741 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
    37801 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
    37861 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
    37921 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
    37981 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
    38041 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
    38101 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
    38161 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
    38221 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
    38281 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
    38341 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
    38401 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
    38461 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
    38521 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
    38581 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
    38641 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
    38701 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
    38761 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
    38821 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
    38881 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
    38941 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
    39001 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
    39061 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
    39121 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
    39181 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
    39241 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
    39301 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
    39361 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
    39421 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
    39481 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
    39541 ctccagcaat tgcttacggc taagattcgc cgcaaggtaa gggcgatgca gacgcacagg
    39601 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
    39661 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
    39721 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
    39781 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
    39841 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
    39901 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
    39961 ggattggcat gtggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
    40021 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
    40081 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
    40141 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg taggcgcgca ggtcagcggt
    40201 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
    40261 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
    40321 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
    40381 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
    40441 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
    40501 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
    40561 ggcggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
    40621 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
    40681 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
    40741 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
    40801 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
    40861 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
    40921 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
    40981 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
    41041 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
    41101 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
    41161 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
    41221 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
    41281 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
    41341 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgctttcgc
    41401 atttaaccgc cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
    41461 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
    41521 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
    41581 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
    41641 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
    41701 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
    41761 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
    41821 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
    41881 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
    41941 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
    42001 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
    42061 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
    42121 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
    42181 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
    42241 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
    42301 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
    42361 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
    42421 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
    42481 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
    42541 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
    42601 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
    42661 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
    42721 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
    42781 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
    42841 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
    42901 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
    42961 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
    43021 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
    43081 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
    43141 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
    43201 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
    43261 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaaaccttt ggcattgttg
    43321 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
    43381 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
    43441 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
    43501 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
    43561 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
    43621 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
    43681 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
    43741 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
    43801 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
    43861 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
    43921 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
    43981 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
    44041 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
    44101 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
    44161 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
    44221 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
    44281 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
    44341 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
    44401 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
    44461 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
    44521 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
    44581 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
    44641 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
    44701 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
    44761 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
    44821 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
    44881 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
    44941 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
    45001 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
    45061 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
    45121 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
    45181 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcgggcgct
    45241 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
    45301 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
    45361 accctgaaaa acctcggcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
    45421 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
    45481 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
    45541 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
    45601 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
    45661 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
    45721 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
    45781 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
    45841 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
    45901 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
    45961 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
    46021 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
    46081 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
    46141 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
    46201 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
    46261 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
    46321 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
    46381 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
    46441 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
    46501 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
    46561 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
    46621 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
    46681 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
    46741 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
    46801 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    46861 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    46921 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    46981 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    47041 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    47101 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    47161 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    47221 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    47281 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    47341 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    47401 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    47461 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    47521 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    47581 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    47641 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    47701 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    47761 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    47821 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    47881 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    47941 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    48001 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    48061 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    48121 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    48181 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    48241 ggcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    48301 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    48361 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    48421 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    48481 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    48541 taacagcgca cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    48601 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    48661 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    48721 cagcgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    48781 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    48841 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    48901 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    48961 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    49021 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    49081 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    49141 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    49201 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    49261 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    49321 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    49381 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    49441 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    49501 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    49561 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    49621 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    49681 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    49741 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    49801 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    49861 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    49921 gagctgcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    49981 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    50041 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    50101 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    50161 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    50221 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    50281 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    50341 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    50401 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    50461 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    50521 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    50581 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    50641 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    50701 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    50761 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    50821 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    50881 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    50941 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    51001 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    51061 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    51121 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    51181 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    51241 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    51301 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    51361 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    51421 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    51481 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    51541 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    51601 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    51661 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    51721 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    51781 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    51841 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    51901 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    51961 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    52021 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    52081 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    52141 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    52201 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    52261 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    52321 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    52381 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    52441 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    52501 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    52561 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    52621 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    52681 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    52741 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    52801 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    52861 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    52921 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    52981 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    53041 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaattgatg
    53101 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaaacagta
    53161 ttcttatatg cgcataacca tgtatgtaaa taccatgttt accgtgctag tgaaatctac
    53221 gtatggcgtg gacagacgcc attcgtgatg tcgatagctg ccacaaggca acggtcttct
    53281 caccgtagac ccaggcattg cgcgccgtga atcttcatga tttcggtcta tcgtgacggg
    53341 tagcgactct gaacagtgat gtttcagggt cagacaggag tagggaagga atacagagag
    53401 acaataataa tggtagatag caagaagcgc cctggcaaag atctcgaccg tatcgatcgt
    53461 aacattctta atgagttgca aaaggatggg cgtatttcta acgtcgagct ttctaaacgt
    53521 gtgggacttt ccccaacgcc gtgccttgag cgtgtgcgtc ggctggaaag acaagggttt
    53581 attcagggct atacggcgct gcttaacccc cattatctgg atgcatcact tctggtattc
    53641 gttgagatta ctctgaatcg tggcgcaccg gatgtgtttg aacaattcaa taccgctgta
    53701 caaaaacttg aagaaattca ggagtgtcat ttagtatctg gtgatttcga ctacctgttg
    53761 aaaacacgcg tgccggatat gtcagcttac cgtaagttgc tgggggaaac cctgctgcgt
    53821 ctgcctggcg tcaatgacac acggacatac gttgttatgg aagaagtcaa gcagagtaat
    53881 cgtctggtta ttaagacgcg ctaacacgga acaggtgcaa aatcggcgta ttttgattac
    53941 actcctgtta atccatacag caacagtact ggggtaacct ggtactgttg tccgttttag
    54001 catcgggcag gaaaagcctg taacctggag agcctttctt gagccaggaa tacactgaag
    54061 acaaagaagt cacattgaca aagttaagta gcggccgccg ccttctggag gcgttgctga
    54121 tccttattgt cctgtttgcc gtctggttga tggctgcctt actaagcttt aacccttcgg
    54181 accccagctg gtcgcaaacg gcctggcatg aacctatcca taatttaggt gggatgcccg
    54241 gtgcgtggtt ggcagatacg ctgttcttta tttttggcgt gatggcttac accattcccg
    54301 tcattattgt cggcggttgt tggtttgcct ggcgtcatca gtccagcgac gaatacattg
    54361 attattttgc cgtttcgcta cgcatcattg gcgttttggc gctcatcctt acctcctgtg
    54421 gtctggcggc aatcaacgct gacgatatct ggtattttgc ctccggtggc gtcattggca
    54481 gcttactaag cactacgcta caaccactgc tacacagtag cgggggaact attgcgctgc
    54541 tctgcgtttg ggcagcgggc ctgacgttgt tcaccggttg gtcatgggtg accattgctg
    54601 aaaaactcgg cggctggatt ttaaacattc tcaccttcgc cagtaatcgt acccgtcgcg
    54661 atgatacctg ggtcgatgaa gatgagtatg aagacgacga agagtatgaa gatgaaaatc
    54721 acggcaaaca gcatgaatca cgccgtgccc gtattcttcg cggcgcgcta gcgcgtcgta
    54781 aacggttggc ggaaaaattc attaatccga tggggcggca aacagacgct gcgttgttct
    54841 ccggtaagcg gatggatgat gacgaagaga ttacctacac tgcacgcggt gtggctgccg
    54901 acccggacga cgtcctattt ttgggcaatc gtgcaacgca gccagaatat gacgaatacg
    54961 atccattatt aaacggtgcg ccaattaccg aacctgtcgc tgtagcagct gctgctacca
    55021 cggcgacaca aagctgggct gcgccggttg aacctgtgac tcagacgccg cctgttgcct
    55081 ctgttgatgt tccaccttcg caacctacag tagcctggca gcctgtaccg ggtccacaaa
    55141 cgggagagcc ggttattgct cctgcaccgg aaggttaccc acagcagtca caatatgcgc
    55201 agcctgcagt gcaatataat gagccgctgc aacaaccagt acagccgcag cagccgtatt
    55261 atgcacctgc agctgaacaa cctgcgcaac aaccttacta cgctcctgcg gcggaacaac
    55321 ctgttcagca accgtattac gcaactgcgc cggaacagcc tgcgcaacag ccgtattatg
    55381 cccctgcgcc agaacaaccg gtggcaggta acgcctggca agccgaagag cagcaatcca
    55441 tttttgctcc acagtctaca taccagactg agcaaactta tcagcagcca gccgctcagg
    55501 agccgttgta ccaacagccg caacccgttg aacagcagcc tgttgtggag cctgaacccg
    55561 ttgtagaaga gacaaaaccc gcgcgtccgc cgctttacta ttttgaagaa gtggaagaga
    55621 agcgagcccg tgaacgtgaa caacttgcgg cctggtatca accgattcca gaaccggtta
    55681 aagaaccaga accgatcaaa tcttcgctga aagcaccttc tgttgcagca gtacctccag
    55741 tagaagccgc tgccgctgtt tctccgctgg catctggcgt gaaaaaagcg acactggcga
    55801 cgggggctgc cgcaaccgtt gccgcgccag tcttcagtct ggcaaatagc ggtggaccgc
    55861 gtccgcaggt caaagagggg attggcccgc agttgccacg accgaaacgt atccgcgtgc
    55921 caactcgtcg tgaattggcg tcttacggta ttaagctgcc ctcacagcgt gcggcggaag
    55981 aaaaagcccg tgaagcccgg cgcaatcagt atgattctgg cgatcagtat aacgatgatg
    56041 aaatcgatgc gatgcagcag gatgaactgg cacgtcagtt cgcccagaca cagcagcaac
    56101 gctatggcga acagtatcaa catgatgtgc ccgtaaacgc agaagatgca gatgctgcgg
    56161 cagaggctga actggctcgt cagtttacgc aaactcaaca acaacgttat tccggcgaac
    56221 aaccggctgg ggcgaatccg ttctcgctgg atgattttga attttcgcca atgaaagcgt
    56281 tgctggatga tggtccacac gaaccgctgt ttacgccaat tgttgaacct gtacagcagc
    56341 cgcaacaacc ggttgcacca cagcagcagt atcagcagcc acaacagccg gttgcgccac
    56401 agccgcagta tcagcagcca caacagcagg ttgcgccgca gccacaatat cagcagccgc
    56461 aacaaccggt tgcgccacag cagcaatatc agcagccgca acaaccagtt gcgccgcagc
    56521 cgcagtatca acagccgcaa caaccggttg caccgcagcc gcagtatcag cagccacaac
    56581 agccagttgc gccacagccg caggataccc tgcttcatcc gctgttgatg cgtaatggcg
    56641 acagccgtcc gttgcacaaa ccgacgacgc cgctgccttc tctggatttg ctgacaccgc
    56701 cgccgagcga agtggagccg gtagatacct ttgcgcttga acaaatggcg cgtctggtgg
    56761 aagcgcgtct ggctgatttc cgtattaaag ccgatgtcgt taattactct ccggggccag
    56821 ttatcactcg ctttgaactt aacctggctc ctggcgtaaa agcggcgcgc atttctaact
    56881 tgtcacggga ccttgcccgt tcactttcga cggtggcggt gcgtgtcgtt gaagttattc
    56941 ctggcaaacc ctatgtaggt ctggagttac caaataaaaa acgacaaacc gtttatctgc
    57001 gcgaagtttt ggataacgcc aaattccgcg ataatccgtc gccattaacc gtggtgctgg
    57061 gtaaagatat cgccggtgag ccggtggttg ccgatctggc gaaaatgccg cacttgttgg
    57121 ttgcggggac taccggttcc ggtaaatctg tcggtgtgaa cgcgatgatc ctgagcatgc
    57181 tttataaagc acagccagaa gatgtgcgtt tcatcatgat cgacccgaaa atgctggagc
    57241 tttcggttta tgaaggcatt ccgcacctgt taacggaagt cgttactgat atgaaagatg
    57301 ccgccaacgc gctgcgctgg tgtgttaacg agatggagcg tcggtataaa ctgatgtctg
    57361 cgctgggtgt gcgtaatctg gcgggttata acgaaaaaat tgctgaagcc gatcgcatga
    57421 tgcgtccgat tccagacccg tactggaagc cgggtgacag tatggatgcc cagcatccgg
    57481 tgctgaaaaa agaaccatac attgtcgtgt tggttgacga atttgccgac ctgatgatga
    57541 cggtaggtaa aaaagtggaa gagctgatag cacgtctggc gcaaaaagcc cgtgccgcgg
    57601 gtatccacct cgtactggca actcagcgtc catcggttga tgttattact ggtctgatta
    57661 aagcgaatat tccgacccgt atcgccttta ccgtatccag taagattgac tcacgtacca
    57721 ttcttgatca ggctggcgcg gaatcactgc tgggtatggg ggatatgctc tactctgggc
    57781 cgaactccac gttgccggta cgtgtccatg gtgcttttgt tcgcgatcag gaagttcatg
    57841 ccgtggtgca ggactggaaa gcgcgtggtc gcccacagta tgttgatggc atcacctccg
    57901 acagcgaaag cgaaggtggt gcgggtggtt tcgatggcgc tgaagaactg gatccgttgt
    57961 tcgatcaggc ggtgcagttt gtcactgaaa aacgcaaagc gtcaatttct ggcgtacagc
    58021 gtcagttccg cattggttat aaccgtgcag cgcgtattat cgaacagatg gaagcgcagg
    58081 ggattgtcag cgaacagggg cacaacggta atcgtgaagt gctggcccca ccgccgtttg
    58141 actaattaat gcattgccgg ataaggcgcg gtagcgtcgc atccggcact ctatcaactg
    58201 aaaattcagt attttcttct ttcctcaagc tgattattag cctggaatag agagtagagg
    58261 gaactcccga tcgggagtga cgtaatttga ggaataatga tgaaaaaaat tgctatcacc
    58321 tgtgcattac tctcaagctt agtagcaagc agcgtttggg ctgatgccgc aagcgatctg
    58381 aaaagccgcc tggataaagt cagcagcttc cacgccagct tcacacaaaa agtgactgac
    58441 ggtagcggcg cggcggtgca ggaaggtcag ggcgatctgt gggtgaaacg tccaaactta
    58501 ttcaactggc atatgacaca acctgatgaa agcattctgg tttctgacgg taaaacactg
    58561 tggttctata acccgttcgt tgagcaagct acggcaacct ggctgaaaga tgccaccggt
    58621 aatacgccgt ttatgctgat tgcccgcaac cagtccagcg actggcagca gtacaatatc
    58681 aaacagaatg gcgatgactt tgtcctgacg ccgaaagcca gcaatggcaa tctgaagcag
    58741 ttcaccatta acgtgggacg tgatggcaca atccatcagt ttagcgcggt ggagcaggac
    58801 gatcagcgca gcagttatca gctgaaatcc cagcaaaatg gggctgtgga tgcagcgaaa
    58861 tttaccttca ccccgccgca aggcgtcacg gtagatgatc aacgtaagta gaggcacctg
    58921 agtgagcaat ctgtcgctcg atttttcgga taatactttt caacctctgg ccgcgcgtat
    58981 gcggccagaa aatttagcac agtatatcgg tcagcaacat ttgctggctg cggggaagcc
    59041 gttgccgcgc gctatcgaag ccgggcattt gcattctatg atcctctggg ggccaccggg
    59101 taccggcaaa acaaccctcg ctgaagtgat tgcccgctat gcgaacgctg atgtggaacg
    59161 tatttctgcc gtcacctctg gcgtgaaaga gattcgcgag gcgatcgagc gcgcccggca
    59221 aaaccgcaat gcaggtcgcc gcactattct ttttgttgac gaagttcacc gtttcaacaa
    59281 aagccagcag gatgcatttc tgccacatat tgaagacggc accatcactt ttattggcgc
    59341 aaccactgaa aacccgtcgt ttgagcttaa ttcggcactg ctttcccgtg cccgtgttta
    59401 tctgttgaaa tccctgagta cagaggatat tgagcaagta ctaactcagg cgatggaaga
    59461 caaaacccgt ggctatggtg gtcaggatat tgttctgcca gatgatacac gacgtgccat
    59521 tgcagaactg gtgaatggcg acgcgcgccg ggcgttaaat acgctggaaa tgatggcgga
    59581 tatggccgaa gtctatgata gcggtaagcg ggtcctgaag cctgaattac tgaccgaaat
    59641 cgccggtgaa cgtagcgccc gctttgataa caaaggcgat cgcttttacg atctaatttc
    59701 cgcactgcat aagtcggtac gtggtagcgc acccgatgcg gcgctgtact ggtatgcgcg
    59761 aattattacc gctggtggcg atccgttata tgtcgcgcgt cgctgtctgg cgattgcgtc
    59821 tgaagacgtc ggtaatgccg atccacgggc gatgcaggtg gcaattgcgg cctgggattg
    59881 ctttactcgc gttggcccgg cggaaggtga acgcgccatt gctcaggcga ttgtttacct
    59941 ggcctgcgcg ccaaaaagca acgctgtcta cactgcgttt aaagccgcgc tggccgatgc
    60001 tcgcgaacgc ccggattatg acgtgccggt tcatttgcgt aatgcgccga cgaaattaat
    60061 gaaggaaatg ggctacgggc aggaatatcg ttacgctcat gatgaagcaa acgcttatgc
    60121 tgccggtgag gtttacttcc cgccggaaat agcacaaaca cgctattatt tcccgacaaa
    60181 caggggcctt gaaggcaaga ttggcgaaaa gctcgcctgg ctggctgaac aggatcaaaa
    60241 tagccccata aaacgctacc gttaatgtta tcgttgcggt aatgttgtta ctgtatccct
    60301 gtggtcgcag gctgtggcca catctcccat ttaattcgat aagcacagga taagcatgct
    60361 cgatcccaat ctgctgcgta atgagccaga cgcagtcgct gaaaaactgg cacgccgggg
    60421 ctttaagctg gatgtagata agctgggcgc tcttgaagag cgtcgtaaag tattgcaggt
    60481 caaaacggaa aacctgcaag cggagcgtaa ctcccgatcg aaatccattg gccaggcgaa
    60541 agcgcgcggg gaagatatcg agcctttacg tctggaagtg aacaaactgg gcgaagagct
    60601 ggatgcagca aaagccgagc tagatgcttt acaggctgaa attcgcgata tcgcgctgac
    60661 catccctaac ctgcctgcag atgaagtgcc ggtaggtaaa gacgaaaatg acaacgttga
    60721 agtcagccgc tggggtaccc cgcgtgagtt tgactttgaa gttcgtgacc acgtgacgct
    60781 gggtgaaatg cactctggcc tcgactttgc agctgcagtt aagctgactg gttcccgctt
    60841 tgtggtaatg aaagggcaga ttgctcgcat gcaccgcgca ctgtcgcagt ttatgctgga
    60901 tctgcatacc gaacagcatg gctacagtga gaactatgtt ccgtacctgg ttaaccagga
    60961 cacgctgtac ggtacgggtc agctgccgaa atttgctggc gatctgttcc atactcgtcc
    61021 gctggaagaa gaagcagaca ccagtaacta tgcgctgatc ccaacggcag aagttccgct
    61081 gactaacctg gtgcgcggtg aaatcatcga tgaagatgat ctgccaatta agatgaccgc
    61141 ccacacccca tgcttccgtt ctgaagccgg ttcatatggt cgtgacaccc gtggtctgat
    61201 ccgtatgcac cagttcgaca aagttgaaat ggtgcagatc gtgcgcccag aagactcaat
    61261 ggcggcgctg gaagagatga ctggtcatgc agaaaaagtc ctgcagttgc tgggcctgcc
    61321 gtaccgtaaa atcatccttt gcactggcga catgggcttt ggcgcttgca aaacttacga
    61381 cctggaagta tggatcccgg cacagaacac ctaccgtgag atctcttcct gctccaacgt
    61441 ttgggatttc caggcacgtc gtatgcaggc acgttgccgc agcaagtcgg acaagaaaac
    61501 ccgtctggtt cataccctga acggttctgg tctggctgtt ggtcgtacgc tggttgcagt
    61561 aatggaaaac tatcagcagg ctgatggtcg tattgaagta ccagaagttc tgcgtccgta
    61621 tatgaacgga ctggaatata ttggctaata cccaattttt ctgaatctaa aaagcgcctt
    61681 tagggcgctt tttttgtctc cctttgatac cgaacaataa ttactcctca cttacacgta
    61741 atactacttt cgagtgaaaa tctacctatc tctttgattt tcaaattatt cgatgtatac
    61801 aagcctatat agcgagctgc tatagaaata attacacaat acggtttgtt actggaatca
    61861 atcgtgatca agcttgagtg agccattatg aaaacgaaaa tccctgatgc ggtattggct
    61921 gctgaggaga gtcgccgtgg tttggtaaaa acgacagcga tcggcggcct ggcaatggcc
    61981 agcagcgcat taacattacc ttttagtcgg attgcgcacg ctgtcgatag cgccattcca
    62041 acaaaatcag acgaaaaggt tatctggagc gcctgtacag ttaactgtgg tagtcgctgc
    62101 ccgctacgta tgcacgtcgt ggacggtgaa atcaaatatg tcgaaacgga caataccggc
    62161 gatgacaatt acgacggcct gcactaggtt cgcgcctgcc tgcgtgggcg ttccatgcgt
    62221 cgccgtgtct acaatccgga ccgcctgaaa tatccgatga aacgagtcgg ggcgcgcggt
    62281 gaaggcaaat tcgagcgcat tagctgggaa gaagcctacg acatcatcgc gaccaatatg
    62341 cagcgcctga tcaaagagta cggcaacgag tctatctatc tgaactatgg caccggtacg
    62401 ctgggcggca ccatgacccg ctcctggccg ccgggaaata ccctggtcgc gcgcctgatg
    62461 aactgctgcg gcggttatct taaccattac ggtgactact cttccgcgca aattgcggaa
    62521 ggtttgaacc atacctacgg cggctgggca gatggtaaca gcccgtcgga tatcgaaaac
    62581 agtaaattgg tagtgctgtt tggtaataac ccaggcgaaa cgcgaatgag tggcggtggg
    62641 gtgacttact atcttgaaca ggcacgccag aaatctaatg ctcgcataat catcatcgat
    62701 ccgcgctata ccgacaccgg tgccgggcgc gaagatgagt ggatccctat tcgtccagga
    62761 acagatgccg cactggttaa cggtctggcg tacgtcatga tcactgaaaa cctggtggat
    62821 caggcattcc tcgataaata ttgcgttggc tatgacgaga aaaccctgcc cgccagtgcg
    62881 ccgaaaaatg gtcattataa agcttatatt ctgggtgaag ggccagatgg cgtggctaaa
    62941 acaccggaat aggcctcgca aatcaccggt gttccggcag acaaaatcat caaactggct
    63001 cgtgaaatcg gcagtaccaa accggcgttt atcagccagg gatggggccc gcagcgtcac
    63061 gctaacggtg aaatcgcaac ccgtgctatc tcgatgctgg cgattctgac cggtaacgtt
    63121 ggtattaatg gagggaacag cggcgcgcgt gaaggttcat acagcttacc gtttgtccgt
    63181 atgccgacct tggaaaaccc gatccagacc agcatttcga tgtttatgtg gaccgatgcc
    63241 attgaacgtg gcccggaaat gacggcgctg cgtgatggtg tgcgcgggaa agataagctg
    63301 gatgtgccga tcaaaatgat ctggaactat gccggtaact gcctgattaa ccagcattct
    63361 gaaatcaacc gtacccatga aatcctccag gatgataaga agtgcgagct gattgtggtt
    63421 atcgactgcc acatgacctc atcggcgaaa tatgctgaca tcctgctgcc tgactgcacc
    63481 gcttccgaac agatggactt tgcactggat gcatcctgcg ggaatatgtc ttacgtgatt
    63541 ttcaacgatc aggtgattaa accgcgcttt gaatgtaaga ccatctatga aatgaccagc
    63601 gaactggcaa aacgtcttgg cgttgagcaa cagtttactg aaggccgtac ccaggaagag
    63661 tggatgcggc atctgtatgc ccagtcgcgg gaagcgattc ctgaactgcc aacgtttgaa
    63721 gagttccgca agcaggggat ctttaaaaag cgcgacccac aagggcatca cgttgcttat
    63781 aaagccttcc gtgaagatcc gcaggcaaac ccactgacta cgccatcggg caaaattgag
    63841 atttattcgc aggcgctggc tgacattgcc gctacctggg aattgccaga aggcgatgtg
    63901 atcgatccac tgccgatcta cacgccgggc tttgaaagtt atcaggatcc gctgaacaaa
    63961 cagtatccgc tgcagcttac aggtttccac tataaatctc gcgttcactc aacttacggc
    64021 aacgttgatg tgctgaaagc ggcttgccgt caggaaatgt ggatcaaccc gcttgatgcc
    64081 caaaaacgcg gtatccacaa cggcgataaa gtcaggatct ttaacgatcg tggtgaggtt
    64141 catattgagg cgaaagtgac gccacgaatg atgccgggcg tggtcgcact gggtgaaggc
    64201 gcctggtatg acccggatgc aaaacgtgtc gataagggcg gttgtatcaa cgtactgacc
    64261 actcaacgtc cgtctcctct cgctaagggg aatccgtcac atacaaacct tgttcaggtt
    64321 gaaaaggtgt aaggagtaac cgatgacaac ccagtatgga ttttttattg attccagccg
    64381 ttgcaccggt tgcaaaacct gcgagctggc ttgtaaagac tacaaagatt tgacgccaga
    64441 agtcagtttc cgccgcattt atgaatatgc tggcggcgac tggcaggaag ataacggtgt
    64501 ctggcaccag aacgtgtttg cctactatct gtcgatttcg tgcaaccact gcgaagatcc
    64561 ggcttgtact aaagtctgcc cgagcggtgc gatgcataaa cgcgaagatg gttttgttgt
    64621 ggtcgatgaa gatgtatgca ttggctgcca ttactgtcat atggcttgcc cgtatggcgc
    64681 accgcaatat aacgaaacga aaggccatat gaccaaatgc gatggttgtt atgaccgtgt
    64741 tgcggagggt aaaaagccga tctgtgttga atcttgcccg ctgcgggcgc tggatttcgg
    64801 ccctatcgac gagctgcgta aaaaacatgg cgatctggcg gccgttgcgc cgttgccgcg
    64861 agctcacttt accaaaccga atattgtgat caaacccaat gccaatagcc gcccgacagg
    64921 ggataccact ggctatctgg caaacccgaa ggaggtgtga gatgggaagt ggatggcatg
    64981 aatggccgct gatgatcttc acggtcttcg ggcaatgtgt ggcgggtggt tttatcgttc
    65041 tggctttggc gctgctcaaa ggcgacctgc gagcagaagc ccagcagcgt gttatcgcct
    65101 gcatgtttgg cttatgggta ctgatgggca ttggctttat cgcctctatg ctccatcttg
    65161 gttcaccgat gcgcgccttt aactcgctca accgggtagg ggcttcagca ctcagtaacg
    65221 aaatcgccag cggttcgatc ttttttgctg taggcggcat cggctggttg ctggcaatgc
    65281 tgaaaaagct gtcaccggca ttgcgtacgc tatggctggt ggtgacgatg gttcttggcg
    65341 tcgtctttgt ctggatgatg gtgcgtgtgt ataacagcat tgataccgtt ccgacctggt
    65401 acagcatctg gacgccgatg ggcttcttcc tgacgatgtt catgggcggc ccgttactgg
    65461 gttacctgtt gctgagtctg gctggcgtcg atggctgggc gatgcgtctg ctgccagcca
    65521 tttctgtact ggcactggtg gtgagtggcg tgatgtcagt gatgcagggc acagagctgg
    65581 caactattca tagttctgtg cagcaggccg cagcgctggt gccggactat ggtgcgctga
    65641 tgtcctggcg gatcgtgctt ttggccgttg ccctgtgctt gtggattgca ccacagctaa
    65701 aaggttatca gcctgcggtt ccgctactat ccgtctcatt cattctgctt ctggcagggg
    65761 aattaatcgg tcgcggcgta ttctatggtt tgcatatgac cgtggggatg gccgtcgcaa
    65821 gctaatcagt aacaaccggg gtttcggccc cggttctctt ttatttctgc ttcgttaacg
    65881 tgtcataact ggtcatcaaa ttacgataat caggaatatg gttagagaac aacgtcgcca
    65941 gtccttcaat atcattacgc cagtcacggt gcagctcgca agccacgccg aaccatgtca
    66001 tcaattgcgc accagcttgc gacatgcgat cccacgccga atgccgggta atttcattaa
    66061 aggtccctga tgcatcagtc acgacaaaga catcaaaccc ttcttcaatg gctgaaagcg
    66121 ccgggaatgc cacgcaaact tcggttacca caccggcaat aattaactgt tttttacctg
    66181 tcgctttgac tgcttttaca aaatcttcgt tatcccaggc gttaatattg cccgggcgag
    66241 caatgtaagg tgcatcgggg aattgtgctt tcagttctag aactaatggg ccgttaggac
    66301 cggtttcaaa actggtggta agaatagttg gtaagttgaa atacttagcc aggtcaccca
    66361 gcgccagcac gttattttta aacttatcgg gttcgatatc ccgtacaagg gaaagtaaac
    66421 cagcctggtg atcaacaagc aaaacggcag catcattttt atcaagacga acatacggtt
    66481 tggtcatcct cttctccttt cgagagtcag tggtacgtct gaaatcggtc agacgcgatg
    66541 cattgctctg aaagcataga cgggaaatat gagtttgctg tgaccatgaa atttttcgac
    66601 tgaacgaagc gtatgaaatt tgtgttagat caataaaaac aatcagatga gattaatcat
    66661 ctcttcagca ttagcgttat gaatgagtaa tataaatcga taataccgcc gctgcgatat
    66721 gcgcgtattt tcacccattg acaatgtttt tggcggtggc atgatgcgca tgaaatttga
    66781 acttcctcac ggttttaatt catgtccacg tatacccggc ctgtcatgct tttactgtct
    66841 ggcctgcttt tgttgactct ggcgatcgcg gtgttaaata cactcgtgcc gctttggctc
    66901 gcccaggaac acatgtccac atggcaggta ggcgttgtca gctcatccta ttttaccggc
    66961 aaccttgtcg gtacattgct gacagggtat gtcattaaac gcattggctt taaccgcagc
    67021 tattatctgt cctccttcat ttttgctgct ggctgtgccg gccttggcct gatgattggt
    67081 ttctggagct ggttggcgtg gcgttttgtc gcgggcgtcg gctgtgccat gatttgggtg
    67141 gttgttgaga gcgcgctgat gtgcagtggg acgtcacgta accgtgggcg tttgcttgct
    67201 gcgtatatga tggtttatta cgtgggaacg tttttaggcc agttactggt cagcaaagtc
    67261 tcaaccgagc tgatgagcgt attgccgtgg gttacaggtt tgacattggc agggatctta
    67321 ccgctgttgt ttacgcgtgt cctgaatcag caggctgaaa accatgattc gacgtcaatt
    67381 acggcaatgc taaaactccg tcaggcgcgg cttggcgtga atggctgcat tatctcagga
    67441 atcgttctgg gatctctata tggcctgatg cctctgtacc tcaatcacaa aggggtgagc
    67501 aatgccagta ttggtttctg gatggcggta ctggtcagtg cgggtatcct cggacaatgg
    67561 ccgattggac gtctggcgga taagtttggt cgactgctgg ttttgcgtgt tcaggtcttt
    67621 gtcgtcattc tcggcagtat tgcgatgctt agccaggcgg cgatggcccc agcgttattc
    67681 atcctcggtg ccgctggctt tacgctatat ccggtggcga tggcctgggc ttgtgagaaa
    67741 gttgaacatc atcaactggt ggcgatgaac caagccttac tgttgagcta taccgtggga
    67801 agtctgcttg gcccgtcatt taccgctatg ctaatgcaga atttctccga taatttattg
    67861 tttatcatga tcgccagcgt atcgtttatc tatttgctga tgctgcttcg caacgccggt
    67921 catacgccga aacccgttgc tcacgtgtaa atgaattcaa gcaaagtgtg aacttactgt
    67981 ttcacactct gcttttttgt ttcttctatc tgacttgctt ttttccaaat tttattcgtt
    68041 taaaaataaa atgtgcagca ggttataatt ttgcatttcg ctatttccgc acttcttatt
    68101 tgccgcgcat aatccctcgt tttacagatg cccctttaat tttggcgaag gatttgtcta
    68161 tggctgggaa tgttcaggaa gtaagcgccc catca
//
LOCUS       DADMGC010000005        66599 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.005, whole genome shotgun sequence.
ACCESSION   DADMGC010000005 DADMGC010000000
VERSION     DADMGC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.005"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JNZ73_000332"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JNZ73_000332"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HAY8730743.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JNZ73_000333"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JNZ73_000333"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAY8730744.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JNZ73_000334"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JNZ73_000334"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAY8730745.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JNZ73_000335"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JNZ73_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HAY8730746.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JNZ73_000336"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JNZ73_000336"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HAY8730747.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDASHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JNZ73_000337"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JNZ73_000337"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY8730748.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="JNZ73_000338"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="JNZ73_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HAY8730749.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JNZ73_000339"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JNZ73_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY8730750.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMDICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLN"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JNZ73_000340"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JNZ73_000340"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAY8730751.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JNZ73_000341"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JNZ73_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HAY8730752.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /locus_tag="JNZ73_000342"
     CDS             complement(14847..15542)
                     /locus_tag="JNZ73_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY8730753.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="JNZ73_000343"
     CDS             complement(15665..16702)
                     /locus_tag="JNZ73_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY8730754.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="JNZ73_000344"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="JNZ73_000344"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8730755.1"
                     /translation="MSEIVIRHAETRDYETIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19270)
                     /gene="ggt"
                     /locus_tag="JNZ73_000345"
     CDS             complement(17582..19270)
                     /gene="ggt"
                     /locus_tag="JNZ73_000345"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005071965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAY8730756.1"
                     /translation="MLLCSQEVVLAPPVSYGVEEDVFHPVRAKQGMVASVDATATQVG
                     VDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKNGNTTAIDFREMAPAK
                     ATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGTMPLNKVVQPAFKLAR
                     DGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGGTLVQANLAKSLEMIAE
                     NGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPISGDYRGYQVYSMPPPS
                     SGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADRSEYLGDPDFVKVPWQ
                     ALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTTHYSVVDKDGNAVAVTYTL
                     NTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANAVGPNKRPLSSMSPTI
                     VVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNAPRFHHQWLPDELRVE
                     KGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASDPRSVDDLTAGY"
     gene            19424..19864
                     /locus_tag="JNZ73_000346"
     CDS             19424..19864
                     /locus_tag="JNZ73_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HAY8730757.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19851..20594)
                     /gene="ugpQ"
                     /locus_tag="JNZ73_000347"
     CDS             complement(19851..20594)
                     /gene="ugpQ"
                     /locus_tag="JNZ73_000347"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY8730758.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20616..21503)
                     /gene="ugpC"
                     /locus_tag="JNZ73_000348"
     CDS             complement(20616..21503)
                     /gene="ugpC"
                     /locus_tag="JNZ73_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAY8730759.1"
                     /translation="MADGEFIVMVGPSGCGKSTLLRMVAGLERVTEGDIWINDQRVTE
                     MEPKDRGIAMVFQNYALYPHMSVEENMAWGLKIRGMGKQQIAERVKEAARILELDGLL
                     KRRPRELSGGQRQRVAMGRAIVRDPAVFLFDEPLSNLDAKLRVQMRLELQQLHRRLKT
                     TSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGTPVEVYEKPASLFVASFIGSPAMNLLT
                     GRMNNEGTHFELDGGIVLPLNGGYRQYAGRKMTLGIRPEHIALSSQAEAGGATGASGA
                     PDGRQHAVAASGGKSAASF"
     gene            complement(21583..22428)
                     /gene="ugpE"
                     /locus_tag="JNZ73_000349"
     CDS             complement(21583..22428)
                     /gene="ugpE"
                     /locus_tag="JNZ73_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HAY8730760.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22425..23312)
                     /gene="ugpA"
                     /locus_tag="JNZ73_000350"
     CDS             complement(22425..23312)
                     /gene="ugpA"
                     /locus_tag="JNZ73_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HAY8730761.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23410..24726)
                     /gene="ugpB"
                     /locus_tag="JNZ73_000351"
     CDS             complement(23410..24726)
                     /gene="ugpB"
                     /locus_tag="JNZ73_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HAY8730762.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25123..25836)
                     /gene="livF"
                     /locus_tag="JNZ73_000352"
     CDS             complement(25123..25836)
                     /gene="livF"
                     /locus_tag="JNZ73_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HAY8730763.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSSYLGG"
     gene            complement(25838..26605)
                     /gene="livG"
                     /locus_tag="JNZ73_000353"
     CDS             complement(25838..26605)
                     /gene="livG"
                     /locus_tag="JNZ73_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HAY8730764.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26602..27879)
                     /locus_tag="JNZ73_000354"
     CDS             complement(26602..27879)
                     /locus_tag="JNZ73_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HAY8730765.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27876..28802)
                     /gene="livH"
                     /locus_tag="JNZ73_000355"
     CDS             complement(27876..28802)
                     /gene="livH"
                     /locus_tag="JNZ73_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HAY8730766.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28850..29959)
                     /gene="livk"
                     /locus_tag="JNZ73_000356"
     CDS             complement(28850..29959)
                     /gene="livk"
                     /locus_tag="JNZ73_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HAY8730767.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30383..30766
                     /gene="panM"
                     /locus_tag="JNZ73_000357"
     CDS             30383..30766
                     /gene="panM"
                     /locus_tag="JNZ73_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HAY8730768.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30965..32068)
                     /locus_tag="JNZ73_000358"
     CDS             complement(30965..32068)
                     /locus_tag="JNZ73_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY8730769.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32339..33193)
                     /gene="rpoH"
                     /locus_tag="JNZ73_000359"
     CDS             complement(32339..33193)
                     /gene="rpoH"
                     /locus_tag="JNZ73_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HAY8730770.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33438..34496)
                     /gene="ftsX"
                     /locus_tag="JNZ73_000360"
     CDS             complement(33438..34496)
                     /gene="ftsX"
                     /locus_tag="JNZ73_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HAY8730771.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34489..35157)
                     /gene="ftsE"
                     /locus_tag="JNZ73_000361"
     CDS             complement(34489..35157)
                     /gene="ftsE"
                     /locus_tag="JNZ73_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAY8730772.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35160..36656)
                     /gene="ftsY"
                     /locus_tag="JNZ73_000362"
     CDS             complement(35160..36656)
                     /gene="ftsY"
                     /locus_tag="JNZ73_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAY8730773.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36806..37402
                     /gene="rsmD"
                     /locus_tag="JNZ73_000363"
     CDS             36806..37402
                     /gene="rsmD"
                     /locus_tag="JNZ73_000363"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HAY8730774.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37392..37661
                     /locus_tag="JNZ73_000364"
     CDS             37392..37661
                     /locus_tag="JNZ73_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HAY8730775.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37664..38023)
                     /locus_tag="JNZ73_000365"
     CDS             complement(37664..38023)
                     /locus_tag="JNZ73_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HAY8730776.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38164..38790
                     /locus_tag="JNZ73_000366"
     CDS             38164..38790
                     /locus_tag="JNZ73_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HAY8730777.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38864..41062
                     /gene="zntA"
                     /locus_tag="JNZ73_000367"
     CDS             38864..41062
                     /gene="zntA"
                     /locus_tag="JNZ73_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HAY8730778.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41164..41409)
                     /gene="tusA"
                     /locus_tag="JNZ73_000368"
     CDS             complement(41164..41409)
                     /gene="tusA"
                     /locus_tag="JNZ73_000368"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HAY8730779.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41630..42295
                     /locus_tag="JNZ73_000369"
     CDS             41630..42295
                     /locus_tag="JNZ73_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HAY8730780.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42368..42925
                     /gene="dcrB"
                     /locus_tag="JNZ73_000370"
     CDS             42368..42925
                     /gene="dcrB"
                     /locus_tag="JNZ73_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HAY8730781.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42929..44146)
                     /locus_tag="JNZ73_000371"
                     /pseudo
     CDS             complement(42929..44146)
                     /locus_tag="JNZ73_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            44278..45327
                     /locus_tag="JNZ73_000372"
     CDS             44278..45327
                     /locus_tag="JNZ73_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY8730782.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPDRPKSRLPG"
     gene            45382..45969
                     /gene="acpT"
                     /locus_tag="JNZ73_000373"
     CDS             45382..45969
                     /gene="acpT"
                     /locus_tag="JNZ73_000373"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HAY8730783.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46080..47654
                     /gene="nikA"
                     /locus_tag="JNZ73_000374"
     CDS             46080..47654
                     /gene="nikA"
                     /locus_tag="JNZ73_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HAY8730784.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPLETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47654..48598
                     /gene="nikB"
                     /locus_tag="JNZ73_000375"
     CDS             47654..48598
                     /gene="nikB"
                     /locus_tag="JNZ73_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HAY8730785.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48595..49428
                     /gene="nikC"
                     /locus_tag="JNZ73_000376"
     CDS             48595..49428
                     /gene="nikC"
                     /locus_tag="JNZ73_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HAY8730786.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLILGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49428..50192
                     /gene="nikD"
                     /locus_tag="JNZ73_000377"
     CDS             49428..50192
                     /gene="nikD"
                     /locus_tag="JNZ73_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HAY8730787.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50189..50995
                     /gene="nikE"
                     /locus_tag="JNZ73_000378"
     CDS             50189..50995
                     /gene="nikE"
                     /locus_tag="JNZ73_000378"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HAY8730788.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKNLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51001..51402
                     /gene="nikR"
                     /locus_tag="JNZ73_000379"
     CDS             51001..51402
                     /gene="nikR"
                     /locus_tag="JNZ73_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HAY8730789.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51522..51881)
                     /locus_tag="JNZ73_000380"
     CDS             complement(51522..51881)
                     /locus_tag="JNZ73_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HAY8730790.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51878..52153)
                     /locus_tag="JNZ73_000381"
     CDS             complement(51878..52153)
                     /locus_tag="JNZ73_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAY8730791.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(52212..53336)
                     /locus_tag="JNZ73_000382"
     CDS             complement(52212..53336)
                     /locus_tag="JNZ73_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAY8730792.1"
                     /translation="MRQLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53336..56071)
                     /gene="rbbA"
                     /locus_tag="JNZ73_000383"
     CDS             complement(53336..56071)
                     /gene="rbbA"
                     /locus_tag="JNZ73_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HAY8730793.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56068..57135)
                     /locus_tag="JNZ73_000384"
     CDS             complement(56068..57135)
                     /locus_tag="JNZ73_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY8730794.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKTAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57501..59123)
                     /locus_tag="JNZ73_000385"
     CDS             complement(57501..59123)
                     /locus_tag="JNZ73_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY8730795.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNHGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDSSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGVQFIIDLLKRIMSINKNVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59385..60974)
                     /locus_tag="JNZ73_000386"
     CDS             complement(59385..60974)
                     /locus_tag="JNZ73_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY8730796.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIPLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEESQCYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61470..62389
                     /locus_tag="JNZ73_000387"
                     /pseudo
     CDS             61470..62389
                     /locus_tag="JNZ73_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908812.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
     gene            complement(62476..62969)
                     /locus_tag="JNZ73_000388"
                     /pseudo
     CDS             complement(62476..62969)
                     /locus_tag="JNZ73_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(62923..63687)
                     /locus_tag="JNZ73_000389"
     CDS             complement(62923..63687)
                     /locus_tag="JNZ73_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8730797.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSANLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNGETYAICSEVLLYD"
     gene            63934..64695
                     /locus_tag="JNZ73_000390"
     CDS             63934..64695
                     /locus_tag="JNZ73_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY8730798.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSLQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64802..65314)
                     /locus_tag="JNZ73_000391"
     CDS             complement(64802..65314)
                     /locus_tag="JNZ73_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8730799.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
     gene            complement(65453..>66599)
                     /locus_tag="JNZ73_000392"
     CDS             complement(65453..>66599)
                     /locus_tag="JNZ73_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY8730800.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDQK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        16175 a        17873 c        16342 g        16209 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgtaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggaatgg tttttatctt ccactgcagc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cgaatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccacacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaagtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tgcctggctg attaatgccg aagcgataat catgccgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgagg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta attcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcaga cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagtag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatgtcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tattacgggt aactgttatc ggtaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctggat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tagcaaaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgagacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgaga ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cgcccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgccgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc taaaacaact tcctgagagc
    19261 agagcagcaa tggccacccg gcgtaaaaac gtcggtttta tcatcgttat tctccagaga
    19321 ttaaggggca accccagcta agcctggtat ataactctga attaatcatc gttttgtcgg
    19381 gaagcgagta aacttaaagg atatcttcag aggaggatac gcgatgaaac gacttctgat
    19441 tcttacggca ctcctgccgt ttgtcggctt tgcacagccc attaatactc tgaacaaccc
    19501 taaccagccg gggtatcaga tacccagcca gcagcggatg caaacccaga tgcagactca
    19561 gcaaatccag caaaaaggga tgctgaatca gcaactgaaa acgcaaactc agttgcaaca
    19621 gcagcattta gaaaaccaga taaacaataa ttctcagcgg gtgttgcagt cacagccggg
    19681 ggagcgaaat cccgcccggc agcaaatgct gcccaacacc aacggcggga tgttaaacag
    19741 caaccgtaat ccggatagtt cgttgaatca gcagcatatg ctgccggaga ggagaaacgg
    19801 cgacatgctg aatcagccca gcacgccgca gcctgacatt ccgttgaaaa ctattgggcc
    19861 gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga tgcaatccac gccccagcgc
    19921 agcaactctg ccgcgcgctg gggtttattg acggtataaa ccagaatccg taatccggcg
    19981 tctttcaact gcatcactcg cgctttatcg agtaacttat gattgagatg aatagagacg
    20041 cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc actcatcaag caataaacca
    20101 cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag catcaatctc aaacgatgac
    20161 agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg ccagcgccac cattttgccc
    20221 gttaatggtc cagtgccagt ggtgggtttg atttcgatat tcgccatcat cccgtgttcg
    20281 cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct cacctttaaa cgctttgctg
    20341 taccaactgc ccgcatccac gcgcagtaaa tcctgccagt tcagttcacc cgcgacgccc
    20401 cagccgttgc tggtgcgttc gagattgtcg tcatggagca ggaagatctc gccatctttc
    20461 gataacttcg cgtcaaattc gatcatctta tgaccgtatt ttgccccgac gtcgattgcc
    20521 gccagggtgt tttccggggc cagcttaccg ccgccacgat gagcgacgat gcggggataa
    20581 ggccagttac tcatactcgt tgtcctgttt caccatcaaa aagatgcagc tgattttccg
    20641 ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc ctgatgcgcc agtcgcacca
    20701 ccagcttctg cttgcgagct tagcgcaata tgttccgggc gaatgccgag ggtcatttta
    20761 cgcccggcat actgacggta gccaccgttt agcggtagca cgataccgcc gtccagttcg
    20821 aaatgcgtgc cttcattatt catgcggcct gtcagcaggt tcatcgccgg gctgccgata
    20881 aaactcgcca caaacaggct ggcgggcttt tcgtagactt ccaccggcgt gccaatctgt
    20941 tcggcaacgc cgccgttcat caccattact cgctgggcga gcgtcatcgc ctcaacctga
    21001 tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt gcagctgttg cagttcaaga
    21061 cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg gctcatcaaa caagaacacc
    21121 gccggatcac gcacaatcgc gcgtcccatc gccacacgct ggcgctgacc gccggaaagc
    21181 tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa tgcgcgccgc ttctttaacg
    21241 cgctcggcta tttgctgctt gcccatgccg cgaattttca gcccccacgc catgttttct
    21301 tcgacgctca tatgcggata aagcgcgtaa ttctggaaca ccatcgcaat cccgcgatct
    21361 ttcggctcca tttcggtcac gcgctggtcg ttgatccaga tatcgccttc ggtcaccctc
    21421 tccagcccgg caaccatacg cagcagcgtc gatttcccgc agccagacgg cccgaccatc
    21481 acgataaatt cgccatccgc cacatcaagg gtcagcggtt taatgaccta ggttttgcca
    21541 tcccagcttt tggttactgc ctgtaatttc agtcctgcca tcttatttct cactatcgac
    21601 caggccgcgc acgaaggcac gctgcatcac taaaacaatc actactggtg ggatcagggt
    21661 taacaacatc gccgccatca ctgagttcca ttccgtggtg ccttcgccag tggcgatcat
    21721 ccctttgatc cctgccacgg tggtgccgag atccacgtcg gtaataatca acaacggcca
    21781 caaatactgg ttccagccgt aaataaaggt gatcacaaac agcgccgcca gattagtttt
    21841 ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt gacgcaccgt cgatccgcgc
    21901 cgcttccacc agttcatccg gcagcgtcat aaagaactgg cgaaacagaa acgtggcggt
    21961 cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg tcgagcatct tcaggttggc
    22021 gatgacttcc accgtcggga agatacgtac ttcaaccggc agcatcaggg tgataaaaat
    22081 catccagaag aagaggttac gtagcggaaa acgaaaccag acaatggcaa atgccgagag
    22141 catcgagaca gtaattttgc cgagcgtaat gctgaacgcc atcacaaagc tgttaagcaa
    22201 catccgccag aacggcgcgc tatttgtgcc taccccgttc acccagatgt tgtggatgtt
    22261 ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg gcgtagacgg cctgtttatc
    22321 cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg atcaccgcga tcccgaggat
    22381 cagcatggta tggctgaata tcgtcagcca cggacggttc tcaattattg gtaacgcacc
    22441 ttgctttcaa catagcggaa ctgcaccacc gtcagcacga tgacgaggaa catcaacacc
    22501 accgactgtg cggcagacga agccagatcc agtccggtaa aaccttcgcg gtagatctta
    22561 taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg tggcggcgtc gatcaccggg
    22621 aaggtgtcga agaaggcata caccaggttc actaccagca ggaagaaact caccggggcg
    22681 ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc ctgcaccgtc gatggctgcg
    22741 gcttcgatca acgaacgggg aatggattgc agcgcggcat agaagaacag aaagttgtag
    22801 ctgatttgct tccacaccga ggcaaacacc accagaaaca tcgcctgacc gctattttgc
    22861 gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg tgatcagccc gcgaccgggg
    22921 ttaaacagga agatccacaa tacggcggca acggcgggag ccacggcata cggcagcaac
    22981 attaaggttt ggtagaaacg actgccgcgc acgatgtact ccaccagcgc cgcaaagaac
    23041 agcgatacca gcaaaccgct gacggtgaca aaggtgctga attttatcgt cgtccagaag
    23101 gagtcgagat aatagctgtc atgaaacagc gtgacgaagt tatccagacc gacaaactgg
    23161 ctggagaaac caaacggatc gacgctttgt agcgagtacc acaacgcttc gcccgcaggc
    23221 cagataaaaa agataacggt gatgatgagc tgcggcgcga ccagcagata aggcagccag
    23281 cgcgagcgga acaccggacg ggatgatgac attacggtta attcctgaac tgtgccggat
    23341 gcgcttcgct tatccggcct acacgcgatg ccaggtcaga taagcgaaac gcatccggca
    23401 ttacactgat taagacttcg tcgatttctc aaagcggcgc agtaactggt tcccacgctc
    23461 aactgcggta tccagcgcct gctgtggcgt cttcttaccg gtccacacgc tctcaagctc
    23521 ttcatccaca atcacgcgga tctgcggcat gttgcccaga cgaagccctt tggtgaacgg
    23581 caacggcggc ttgttcagca tctgacgcgt cgcggtatcc gcacctgggt ttttctcgta
    23641 aaagccctgc tcacgggtca ggtcatacgc cgctttggta attggcagat aaccggtttt
    23701 ctgatgccac tcagcagcgt tttctggctt cgcgaggaag tcgagaaact tcgccacgcc
    23761 ggtatacgtt tctttatctt tgccctgcat cacccacagg ctggctccgc cgataatggc
    23821 gttttgtggc gcatctttcg catcggcgtc gtaaggcatc atacctacgc cgtagttaaa
    23881 tttggcgtac tcgcgaatgt tggcaagaga accggaagag gcggtggtca tcgcgcaatc
    23941 accgttatag aacttctcgg tggattcatc cttacgaccg acgtagctga agtcgccctt
    24001 cttgttcatc tcctcgagca tggcgatgtg tttcacctgc tccggcttgt tgaactccag
    24061 caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca aacggcagac cgttccaggc
    24121 gctaaagttt tccagttgga tccagccctg ccagccgctg gcgtagccgc acttaatacc
    24181 ggaggctttc agttttgcgg aatagtccgc cagatcctgc caggttttcg gcggctgttc
    24241 cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa tagagaacgg gagtcgagct
    24301 gttgaacggc tgggagagta agtggccggt tttgctgtcg gagtagtaac cggaaaccgt
    24361 cggcacaaac tgcgactcat cgaactgaat ccctgcctct ttaaacacgt catacaccgg
    24421 tttaatggct ttcgacgcca tcatggtggc ggtgccaact tcataaacct gcaaaatagc
    24481 cggagcgttg ccggtacgaa atgcggcaat ccccgcgctt aaattctgtt cgtagttgcc
    24541 tttataggtc ggtacaattt tgtaatccgg attttcggcg ttaaaacgtt gggccagaga
    24601 atccacctct ttacccagtt ccccttccat agaatgccag aacggaatgg tcgtcactgc
    24661 ctgtgcattc cccattaacg ccagtccgag cgccagtgct gaagctgtat aatgtaacgg
    24721 tttcatcgtt tatctctctt gttgtaccga atgcgcgaat tcacgcgttt tatgctcgcg
    24781 gggtaacatg acatgctcga attacagaaa aataactttt ttgttacatt tgtaagatag
    24841 taaggtgtca gaaagatgac aaggcggtga cggcgtgggt gagggaaaat gggagatggg
    24901 gcacggataa gcggaaaaat agaaggtctg aatcaaactc tacagattac tcatcgtttc
    24961 atgccggatg cggcgtaaac gccttatccg gcctacaaga tcgtgcaaat tcaatatatt
    25021 gcaactcacc cagtaggcct gataagcgca gcgcatcagg caattttgca tttgtcacct
    25081 gtctcaaagg agtcttttga ctccttatca atcaacgtgt tattacccgc ctaaatacga
    25141 acttctcact gcttcgttcg ccagcagcgc gtcgccggta tcggagagca ccacatggcc
    25201 gttttccagc acgtagccgc gatccgccag ctttagcgcc tggttggcgt tctgctcgac
    25261 gaggaagata gtcatcccct gctcgcgcag ctgctcgatg gtgtcgaaaa tttgctggat
    25321 aataatcggc gcaagaccga gcgatggctc atcaagtaac aacaaacgcg gattgctcat
    25381 cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg gacatggtgc ccgcccgctg
    25441 aacacggcgc tcatgcagac gtggaaacag ttcatacacc cactttatgc gctcctgaaa
    25501 ctggtcacgt tcggcaaaga agccgcccat cgccaggttc tcttccaccg tcatccgcga
    25561 gaagacgcga cgcccttccg ggacaatcgc taccgcttcg cgcatgattt tcgccgtctg
    25621 ccagtcggta atgtctttat catcaaacac aattcgcccg ctggtggcgc gcggatcgcc
    25681 gcataacgtg ccgagcaagg tggttttccc cgccccgttc gcgccaatga gagtgacaat
    25741 ctcgccctga ttgatatgca ggctcacctc atgcagcgcc tggattttgc cgtagtgggc
    25801 gctgactttg tcaaaggaca acatgacttt ttccatctta tgcctcacct aaataggcac
    25861 ggatcacgtc cgggttatta cggatttgct ccggcgtacc gtttgccagt ggcgtcccct
    25921 gattgaccac ataaattcgg tctgaaatcc ccatcaccag cttcatatcg tgttcaatca
    25981 acaagatagt ggtgttgtga tgattgcgca gttcggcaat cagctcatcc agctctttgg
    26041 tttcttttgg gttaagacct gccgccggtt cgtcgagcat taaaatctcc ggctgcgtca
    26101 ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata ggccaggtta cttgcctgac
    26161 ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt ggcggcgcgg tcgagcgctt
    26221 cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc agagaacagc ccggttttca
    26281 gttgctgatg ctgcgccacc agcaggtttt caattaccgt catttcacgg aacagacgca
    26341 catgctggaa ggtgcgcacc acgcccatgc gagcaatttg ctgccccggt aaaccttcaa
    26401 gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt gtagaaaccg gtcaggcagt
    26461 taaaaaccgt ggtttttccg gcaccgttag ggccgattaa cgagacgatt tcctgcgggt
    26521 acagttcaag attgacgttg ttcaccgcca gcaggccgcc gaagcgcatc atcagaccgt
    26581 taacagataa taatggctga ctcatgcctg ctctcctttc gctgcgccgt ttttcagctt
    26641 cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc cagatcatca tcagcaccat
    26701 caaaccaccg agcattaaca tgctgtattc gttgaaatca cgcatcaatt cgcgcgacac
    26761 caccagcaga acagccgcca gaatcactgc aaattgcgag cccataccgc cgagcaccac
    26821 tatcgccagc acaaaggccg attcagcaaa ggtgaaggat tccgggctga caaagccctg
    26881 acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg gcgctgatgg taaaagcggt
    26941 cagcttgata cggcgcgggc ttaagcccag cgaacgacag gcgatctcgt cttcacgcaa
    27001 cgcttcccac gcgcggccca gcggcatccg cagcaggcgg ttaatgacaa acaggcttaa
    27061 caccaccagc agcaacgcca ccaggtagag gaagataacg cgatcggacg gatcgtattt
    27121 caggccaaag aaattactga acgtgtccca gccgccttca cgagcggtac ggctgaactc
    27181 gagtccgaag agtgtcggtt tcgggatctg actgattccg ttcgggccgc cggtgatttc
    27241 agtgttattg agcagcaata tgcgcacaat ttcgccgaaa ccaagggtaa cgatcgccag
    27301 atagtcaccg cgcagacgca gcaccggaaa accgagcagg aaacccgccg ccgctgccat
    27361 cagtccggca atcggcaaac aggtccagaa ccccagaccg taatagtgat tgagcagcgc
    27421 aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc agcaccagca gaccagaaag
    27481 accaacaacc acattcagcc cgagaccgag gataatgtag atcatggtca gagtggcgat
    27541 atccaccgtc ccacgtgaca ccataaacgg ccacgccacc acaagcacca gcagcgccac
    27601 gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg ggcagaataa acttcggccc
    27661 ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa agctggaaga aaaagaccac
    27721 cgccgtgccg ataaacatcc actgccaacg gatgtctgaa gccgtgtcga ccaccagttt
    27781 ggtgccatcc agctccagtt gtacgcccat aaagacgccc gccaggacaa agaacatcgc
    27841 ggcagagagc agtgccattg caatatgcat cggtttcata ctttctctac ctccgggcga
    27901 cccagaatac cggtcggcat caccagcagc accagaatca gcagggcgaa tgagaccaca
    27961 tctttatatt ccgtactcag ataggcagaa gagagcgcct ccgcaatccc taggatcagc
    28021 ccgccaatca tcgcccctgg aatgctgcca atcccaccga gcaccgcagc ggtaaaggct
    28081 ttcatcccgg ccataaagcc gatatagggg ttaatgacgc agtagaactg accgagcagt
    28141 acgccagcca ctgccgccat cgccgcgcca ataacaaagg tcaaagcaat cacccggtcg
    28201 gtgttgatgc caagcaggct cgccattttc agatcttccg cgcaggcacg acaggcgcgc
    28261 cccatacggg aatagcgaat gaaaatcgtc agcgccagca tggcgaggaa ggtgacaatc
    28321 cagatcaccg cctgcatggt ggtaatagag gcagagaagt tttcgctatg gcccaccacc
    28381 cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg aaccttcggt caggctgacg
    28441 tagttttgca ggaagatgga cataccgatt gcggaaataa gcgcaatcag gcgcttagag
    28501 ttacgcaccg ggcggtaagc tacccgttcg atactccagc cgtaggcgct ggcgatgacg
    28561 attgcgccga cgaatcccgc cgccaccagc agccagccgg tatcaatgcc catcatcatc
    28621 agcgcggcga tgatcataaa tgagacgtag ctgccaatca tataaacctc gccgtgggcg
    28681 aagttgatca tgccgataat gccgtaaacc atggtgtagc cgatggctat cagcgcgtag
    28741 gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga aatacaaaaa ctgctcagac
    28801 ataaggtaac ctttctaaac ccgcccgcat tttacgggcg gtgggatgat cacttggctg
    28861 ccgtggatga accgtcggcg tgccactgga agacaccaaa atcaaatccc ttaagatcgc
    28921 ctttttcatc ccagttcagc ggcccaatca cggtgtttgc accgttagct tttaaatctt
    28981 tcaccagcgc cagcggctca tcgctgccgg tacgctcaag ggcagttgcc agagattgca
    29041 ctgccgcgta agtgatccag acataaggcc cggacggatc tttcttgtct gctttcagcg
    29101 catcaacgat gccctggttt gccggatcct ggtcatagcg ttttggcata gtgaccaaca
    29161 tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc gttaccgaca ccttctggcc
    29221 ccataaactg ggttttcagg ccaacggaac gggcttggcg cagcatctgc cccatttccg
    29281 ggtagtaacc gccgtagtaa acgaagtcga tattttcttt tttcaggcgg gcgatcagcg
    29341 cggagaaatc tttctccccg gcggtaatac cgtcgaagaa gacgacgttg gcgttagccg
    29401 ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc atactgttgt ttgtcgtgaa
    29461 tgatggcgat gcgctggggc ttcaccgtct caagaatgta ttttgccgcc gtaggcccct
    29521 gggaagagtc cagtccggca gtacgcataa tgtgttgata accgcgttgg gtcagctccg
    29581 ggttggtcgc tcccggcgag atcatcagaa taccttcgtc ttcgtagata tccgacgctg
    29641 gctgggtaga ggaagaacac agatgaccaa taacgtattt aatgccgtcg ttaacgattt
    29701 tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc gtcatattcc acgccaacca
    29761 gcttgtcgcc cttaattccc cctttgacat taatgtcttt aatcgcctga cgcgcgccgt
    29821 taaattccat atcgccccac tgggcaatcg ggccggacat cgcgccgaca acggcgactt
    29881 taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc aatcatccct gcgatgatag
    29941 ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg atgttgtgtt gctttgtttt
    30001 tatgtgttaa caaatcagac tgttcttttt ttatactgca ctgtttttgc ctgtctgatt
    30061 aaggggttag cgcagtattt tgtgatgata gcgattaaaa tccctatttt tcagtcgatt
    30121 aagaacagat aatattctga atttattgat agataaacag aaaaaagtgc ctttgtcagc
    30181 ataaaataac ggcacaaagg gcggaataat tcactatcat tcaggggatt atgctggaca
    30241 tttttcattc tctaatgttt taactttgta attattgctg ttaaaaaatt aatcacctgc
    30301 caaaagaaat aaaaaagaga aagcctccga ttaaattatt tcgctacact ggttccactt
    30361 ttgtgattta cacgggttac ccatgaagct gaccatcatt cgattagaaa actttagcga
    30421 ccaggaccgg attgacctgc aaaagatctg gccggagtat tccccttcct ctttacaggt
    30481 tgacgataac caccgtatct acgccgcgcg ttttaacgag cgtctgcttg cggccgtgcg
    30541 ggtaacctta agcggcaccg agggagcact ggattctcta cgcgtgcggg aagtcacccg
    30601 ccgtcgcggt gtggggcaat atctgctgga agaggttttg cgtaacaatc ctggcgtttc
    30661 atgctggtgg atggcggatg caggcgtgga agatcgcggt gtgatgacgg cgtttatgca
    30721 ggcactggga tttacggcac agcagggcgg ctgggagaag cgttaatcgt caagttgatt
    30781 tcaaaagtga tgaccaacga gaaattgcct gatgcgctac gcttatcagg cctataatgt
    30841 gtgctgcaat ttactgattt ctttggttct tgtaggccgg ataaggcgtt tacgccgcat
    30901 ccggcatgaa gcaacgtatt cgatattagc aatttggcgg caacccaaag ttgccgatta
    30961 acaattactt cgcatcggtc gccgtgccgt tggcgtgcca gtcaaatacg ccgaactcaa
    31021 agccctttag atcgcctttc tcatcccagg tcagtggccc catcacggta tccacggagt
    31081 tcgcttttag gtatttggcg atttcagccg gatcgtcaga ctgattcagg cccgcctgca
    31141 aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc gcttgggtcc tgttttttcg
    31201 ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg atcgtagttc ttcggcttgg
    31261 tcaccagcag cccttccgct gattcgcccg caatgttaga cagcgaaacg ttagccacac
    31321 cttccggccc cataaactga gttttcagcc ctgccgcgcg tgcctgacgc aggatttgcc
    31381 ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat attctctttt ttcagacgcg
    31441 ccaccagcgt tgagaaatct ttttccccgg cggtgatacc atcaaagaac accacgttag
    31501 cattgccttt cttcaggccg tcctgcaccg ctcgtgccag accttcgccg tattgctgtt
    31561 tgtcgtgaac gatagcaata cgctgcggtt tcactttctc aagaatatat ttcgccgccg
    31621 tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat cagctgatag ccacgggcgg
    31681 tcagctccgg cgcggttgcc gctggggtga tcattaaaat gccttcgtct tcgtagatgt
    31741 cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat cacatattta ataccgtcgt
    31801 taacgacttt gttcgccacc gcaaccgcct gtttcgggtc acaggcatcg tcatatttta
    31861 cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt gatatccgca accgcctgct
    31921 ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg accggacatt gcacccacga
    31981 ccgcgacttt aatatcttct gccagagcca tattgctgaa tgccagcgcg atacatcctg
    32041 ccagtaacgc tttacccttt atgttcatcc tgagaatccc cattcttctg gttattacgt
    32101 gtgttgtgat gttgttgttc agcactttat ttcgttttat gcatgactac ccgtgcttta
    32161 gcagcatact ctgctaaaac atacccgatt tttatgatat tggaatagct attttgacag
    32221 tttattaaca atctgcgtgg ggattggcgt tttgccggag ggggaattga ttattgtaga
    32281 ggcccaaaaa acaaaaaccc cggactctca tccagggttc tctgcttaat agcggaaatt
    32341 acgcttcaat agcggcgcga agttttttca tcgcgttctt ttccagttgg cgcacacgtt
    32401 cagcggaaac accgtaacgg tcagccagtt cctgcaacgt ggacttgttg tcttcgtcca
    32461 gccagcgcgc acggatgatg tcctggctgc gttcgtccag accatgcatc gcgtcggtca
    32521 gacggtttgc cgcctgctct tcccagttat catcttcgat gccgtcggca aagttagatg
    32581 atttatcctg cagatagagc accggtgcca tcggctggct gtcggaatcg tcgtcggaag
    32641 acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc catctcacgt acgtctttgc
    32701 tggttacgcc cagttcacgg gccaccattt cgacttcatc ctggttaaac cagcccagac
    32761 gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg cgctttggtg gtcgcaactt
    32821 tgacgatacg ccagttacgc agaacgtatt cgtggatctc tgctttgatc cagtggacgg
    32881 cgaaggagac caggcgcaca cccacttccg ggttaaaacg gcgcactgct ttcatcaggc
    32941 cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc atagcccgca taattacgag
    33001 caatatgaac aacaaaccgc aggtgagaca ggatcagcgt tttagctgct tccagatcgc
    33061 catggtaatg cagcttttca gccagcgccc gctcctcgtc agccgacaac atcggccacg
    33121 cgttagctgc ccggatgtag gaatccaggt tgccaactgg ggctaaagct aaactttgca
    33181 ttttgtcagt cattcaaatc ctctcaatcg atatcttctg gcgcttcagt ggtagcaaca
    33241 actgtgccaa agcttaagag caacgaggtt atcattcact gttttatcag accgtgattt
    33301 tatccacaag ttcaatgcaa gcttgtgaat aaattacgca caaaatgtga catagagatg
    33361 aaataccggg aagagacaac ggggtctctt tccctgctac ggaacccatt gcagggaaag
    33421 agtataacac gcttttatta ttcaggcgta aagtggcgta aatgttgtac cgtggcaagc
    33481 cacgctgcca cccagccaat catcgagcat accagcagca atagcaggca ttcatcgaac
    33541 gataaaccat tgatatcaaa cttcgttccg aaaacctgtg ccacttccgc caccgctgac
    33601 gacaaacgca gtaccagaat ttctgacaaa attaatgaca acaatgcgcc agaaaatccc
    33661 agcagcgcac caccgtacag gaacgggcga aggatgaaac catccgtcgc accaatcagt
    33721 ttctgcacgt tgatggagtc acggcgagca aagatgctca gacgtacgct gttgccgatg
    33781 acgaggaaca ctgccgccac catcaacacg ccgatcatcg ccgaaacgcg cccaaccagc
    33841 ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca tccgcacttc gtcaatgccg
    33901 ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt ccgtcccctg aaaatcgagt
    33961 ttcgggatca ccaccgccac tgccggaagc gggttttctt ccagcatatc cagcgcacca
    34021 ccaaaaccag accagttacg gaactcgccc agtgcgtctt cacgagaaag atagttcact
    34081 ttctccacgc cttgctcggc ctgcaactgt gccaccacgc ccgcagcggc gtcatcgtcc
    34141 agcgtttttt gcaagtaaac agtgatttgc ggtgacggat aatactgcgt cgccgcctga
    34201 ttaacgtttt tgtacaccat ataacagacg ctgggcagcg tcagggagat ggcgataacc
    34261 atcaccgtta aaaacgtggc gaacggtttg cttttcagat cctgcaatgc gccgtggaag
    34321 gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat ttaccggttt tggcgaggat
    34381 ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc cgccgaccga tttacggaag
    34441 cgatccagac gcccaccaaa ctgccgaata tgattgattg catcgcgctt attcatggcc
    34501 cacgcctcca tgcaagtgac catcgctcag ggtgagcatg cgataggaac gccgtgaaat
    34561 caggttaatg tcgtgcgttg ccatcaatac ggttacccca acgcggttaa attcttcaaa
    34621 cagacgtaga atcccttccg acaacgcgtc gtccaggtta ccggtcggtt cgtccgccag
    34681 cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca acacgctgtt gttcaccgcc
    34741 ggaaagctga atagggaagt tcttcgcttt gtccagtagc ccgactttat ccagtgccgc
    34801 cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg ataatcagcg ggatcgccac
    34861 gttatcgtag acagtacggt ccatcagtag atggtgatcc tggaaaatca tgccaatctg
    34921 gcggcgcaga aacggaactt cacggttttt cagacgcgtg atgtcatggc cgctaaacca
    34981 gattttcccg gcgctgggcc gctcaatccc acagatcagc ttcaggaggg tacttttccc
    35041 tgcgccggaa tgaccggtca gaaacgccat ctcacccggc tgcatatgga acgtaacgcc
    35101 ctgcagcgcc tgtctcccac cgagataagc cttgctgaca tgttcaaagc gaatcattgt
    35161 taatcctctc gggcaaaaag tgcctctata aagtcgtccg ccttaaacgg acgcaaatcc
    35221 tcaatacgtt cgccgacacc aatgtagcgg atagggatac caaactggtc agccaccgag
    35281 aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta gcgtgatgcc ggttaagcca
    35341 acagcttcgt ggaacagttt ggcctggctt accgcgttct gcccggtgct ggcatcaata
    35401 gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct tcatcacgcg gacgattttc
    35461 ttcaactctt ccatcagatg cgatttgttc tgcagacgtc cggcggtatc ggcaatcagg
    35521 acgtcgatat tacgcgcttt agctgcctga atggcgtcga agataacaga ggcggaatcc
    35581 gcaccggtat gctgggcaat caccggaata ttgttgcgct gaccccagac ctgaagctgt
    35641 tcaaccgcag ctgcacggaa agtatcgccc gccgccagca tcaccgattt accctgctgc
    35701 tcaaactgac gcgccagctt accaatcgtc gtggttttac ccacaccgtt gacgcccacc
    35761 atcaggatca cgaacggcgt tttatcttca acattcagcg gctcatcgac tttcgccaga
    35821 atctcaccca tctcttcttt cagcaggcca tagagcgcct cggcatcacg aagctgcttg
    35881 cgggatgcgc cttccgtcag attggtgata attttacgtg tggtttccac gcccacatcg
    35941 gcgatcaaca gctgttcttc cagctcttca aacagatcat cgtcgatttt tttaccgcgg
    36001 aacaggctga taaatccgga accgagattt tctttggttt ttaacaggct gcgtttcagg
    36061 cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct gggcaatttc ttcaaccggc
    36121 tgttcttctt ctgccggagg aactaccatc accgcctctt ctgccgcttc ggcagccagc
    36181 gcctgtgctt ccagctcttc gtcggtgatt tcttctttag ccgcttcttc ttctgccgct
    36241 tcgacaatct ctacggtttc cgcttcagcc tgccactctt ctggcgaaac ctcttcggcg
    36301 ttgacgtctt ccggcaacgg cagctcttca cgttcgatag ccactggctc cggcgtttct
    36361 tctacgaccg gttccggctg tgcaacaact tccgcttcag gctgcgcttt ttcactttca
    36421 acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag tttccgcctc ggcttcagta
    36481 tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct tcgcaggctc ttgcgcctga
    36541 acgatttctt ctacaaccgg ttgttcattc tgaacttctg tctctttttc cggggtctgc
    36601 tctttttgac caaagcccag ccaggaaaaa aagccacgtc ttttttcttt cgccatttgc
    36661 gactacactc ctcgctgttg cttcatggca cagcgttaac gctatgtaca tggcagctaa
    36721 aaaaatgatg aaatagtcta tcacttaact taattcacat caccgcctgc aagtatatgt
    36781 tatctggcgg attgagcaat ttatcatgaa aaaaccgaat cattccggca gcggccaaat
    36841 ccgcattatt ggcgggcagt ggcgaggccg taaactcccg gttcctgata gcccaggtct
    36901 gcgccccacc accgaccgcg tacgcgaaac gttgtttaac tggctggctc cggtcattgt
    36961 tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg ctggggctgg aagcgttatc
    37021 gcgctacgct gcgggggcaa cgttgattga gatggatcgc gtggtttctc agcagttaat
    37081 taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg gtgaacagca acgcaatgtc
    37141 attcctggcg caaaaaggca caccgcataa tatcgtgttt gtcgatccac cgttccgccg
    37201 tggcttgtta gaagagacga taaatttact ggaagataac ggctggctgg ctgacgaagc
    37261 cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc actgttccgg caaactggtc
    37321 attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg ctgtatcaac acgaagcaca
    37381 aggagaaagt gatgctgatt aatattggtc gtttgttaat gctctgcgtt tggggatttt
    37441 taatcctcaa tctggtgcat cccttcccgc gcccgctaaa tattttcgtt aacgtggcgc
    37501 tgatttttac cgtgctgatg catggtatgc agctggcgct attgaaatcc actttaccga
    37561 aagatggccc gcagatgacc accgccgaaa aggtgcggat tttccttttc ggcgtgtttg
    37621 aactgctggt ctggcagaag aaatttaaag ttaaaaaata accttattgt tcgccaacaa
    37681 agctaacaaa gcgcgttcct ttatagctca gcgtaccttt atcgcccacg gtcagggcgt
    37741 ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc tccgctttgc ggtttgaagc
    37801 ttgcctcata gcgcatactg gtgcctgccg gagtcacttc ctgctggcgc gaacggcgat
    37861 cgttaatcgg tttttcccgc ttgttgctca ccaccaccag cttttgctgg agcggagcca
    37921 tatcattatc agctttttcc cgtcgctgtt gcataaaacg aaacgatgcg gcgacgacaa
    37981 ttaagccaat gataacaata aagaaaagag gtggtttact catctttatc cctcatcgga
    38041 aaatgcggaa ttaagcatac cctgccagtt atggtgttgt catccgtcca ccctcgccac
    38101 taaactggaa gcaagaccgt aggcattccg cttacgaaaa aataacgaat tcaaggaact
    38161 aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat ggctatctgt ggatgcatcg
    38221 tatcgtgggc caacctggca acgctgggtg tttaaaccgt taacccttct tctcctgctg
    38281 ttactggcct ggcaagcacc gatgttcgac gccattagct atctggtgct ggcgggactg
    38341 tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac gtcaacgtct gatgtacgcc
    38401 atcggcgcgt ttttcctctc gcacctgctg tacaccatct atttcgccag tcagatgacg
    38461 ctctctttct tctggcctct gccactggtg ttgctggttc tgggtgcgct gctactggcg
    38521 attatctgga cgcggctgga agagtaccgt tggcctatct gcacgtttat cggcatgacg
    38581 ctggtgatgg tgtggctggc aggtgaactg tggttcttcc gtccgaccgc tccggcgctc
    38641 tctgcgtttg ttggcgcttc gttgctgttt atcagtaact ttgtctggct ggggagccac
    38701 tatcgccgac gcttccgtgc ggataacgcg attgctgcgg cctgctactt tgccggtcac
    38761 ttcctcatcg tccgctcact gtatctctga taaaacttga ctctggagtc gactccagag
    38821 tgtatccttc ggttaatgag aaaaaactta accggaggat gccatgtcga cacctgacaa
    38881 tcacggcaag aaagcccctc aatttgctgc gttcaaaccg ctaaccacgg tacagaacgc
    38941 caacgactgt tgctgcgacg gcgcatgctc cagctcgcca acgctctctg aaaacgtctc
    39001 cggcacccgc tatagctgga aagtcagcgg catggactgc gccgcctgtg cgcgcaaggt
    39061 agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg caggtgttgt tcgccaccga
    39121 aaaactggtg gttgatgccg acaatgacat ccgtgcccag gttgaatctg cggtgcaaaa
    39181 agcgggctat tccctgcgcg atgagcaggc cgccgacgag ccacaagcat cgcgcctgaa
    39241 agagaatctg ccgctgatta cgcttatcgt gatgatggca atcagctggg gtctggagca
    39301 gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc acgctagttg ggctgtaccc
    39361 gattgctcat caggcattac ggctgatcaa atccggcagc tacttcgcca ttgaaacctt
    39421 aatgagcgta gccgctattg gtgcactgtt tattggcgca acggctgaag ctgcgatggt
    39481 gttgctgctg tttttgattg gtgaacgact ggaaggctgg gccgccagcc gcgcgcgtca
    39541 gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc acgcgcctgc gtaacggtga
    39601 gcgggaagag gtggcgatta acagcctgcg gcctggcgat gtgattgaag tcgccgcagg
    39661 tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt gccagttttg atgaaagcgc
    39721 cctaaccgga gaatccattc cggtggagcg cgcgacgggc gataaagttc ctgcaggagc
    39781 caccagcgta gaccgtctgg taacgctgga agtgctgtca gaaccgggtg ccagcgccat
    39841 tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc cgcgccccca ttgagcggtt
    39901 tatcgaccgt ttcagccgta tttatacgcc agcgattatg gccgtcgccc tgctggtaac
    39961 gctagtgccg ccgctgctgt ttgccgccag ctggcaggag tggatttata aaggtctgac
    40021 gctgctgctg attggttgcc cgtgtgcgtt agttatctca acgcctgcgg cgataacctc
    40081 cgggctggca gcagcagcgc gtcgtggggc gttaattaaa ggcggtgcgg cgctggaaca
    40141 actaggtcgt gttacccaag tggcgtttga taaaaccggt acgctgaccg tcggtaaacc
    40201 gcgcgttacc gcgattcatc cggcaacggg tattagtgaa tctgaactgc tgacactggc
    40261 ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa gccatcgtac gcgaagcaca
    40321 ggttgctgaa ctcgccattc ccaccgccga atcacagcgg gcactggtcg ggtctggcat
    40381 tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct gccgggaaac atcccgctga
    40441 tgcatttgct ggtttgatta atgaactgga aagcgccggg caaacggttg tgctggtagt
    40501 acgtaatgat gacgtgctgg gtatcattgc attgcaggat accctgcgcg ccgacgctac
    40561 aactgccatc agtgaactga acgcgctggg ggttaaagga gtgatcctca ccggcgataa
    40621 tccacgcgca gcggcggcaa ttgccgggga gctggggctg gagtttaaag cgggcctatt
    40681 gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa catgcgcccc tggcgatggt
    40741 cggtgatggt attaacgacg cgccagcgat gaaagctgcc gccatcggga ttgctatggg
    40801 tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca ttaacccata accacctgcg
    40861 cggcctggtg caaatgattg aactggcacg cgccactcac gccaatatcc gccagaacat
    40921 caccattgcg ctgggactga aagggatctt cctcgtcacc acgcttttag ggatgaccgg
    40981 gttgtggctg gcagtgctgg cagatacggg tgcgacggtg ctggtgacgg cgaatgcgtt
    41041 aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca ttgagcgcga tcggtcccct
    41101 cgcccctctg gggagagggt tagggggtga ggaaaaggcg gcatcgaagc caatcagccc
    41161 ctatcaaccg cctttacgaa tcaaataacg ataaggcagt ccatccgtct ctttcgcaac
    41221 cagttcgtgt tccataaagg tacaaaaccc aggaatatcg cgggtagtgg ccggatcgtc
    41281 ggcgataatc agcaacgttt cgccaggctg catattgcgc acggttttgc gcaccatcat
    41341 caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg tggtcagggc tggaaaagag
    41401 atcggtcatt ttcttctcat cacttaaaaa aacggcgcta gtttacgccc tgtgagtccg
    41461 taagcaaacc aggttaacga ttgcgtgaaa attaaccatt gcattgtcaa cgtaaagcag
    41521 tatcatgcgg cggctcgaaa aaagggtaag cacgttatta tgttaaggta acagacgtgt
    41581 cgtacgtatt gggttccctc accccaatgg ttaatcaaaa aggtacaata tgaacgtttt
    41641 ctcgcaaact caacgctata aggcgttgtt ctggttatcg ttatttcatc ttctggtgat
    41701 cacctccagt aactatctgg ttcagcttcc cgtctccatt tttggtttcc ataccacctg
    41761 gggcgcgttt agctttccgt ttatttttct tgctaccgac ctgaccgtgc gtatttttgg
    41821 cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc cctgcgttat taatctccta
    41881 cgtcatctcg tcgctattct atatgggttc ctggcaggga ttcggcgcac tcgcccactt
    41941 caacctgttt gtcgcccgta tcgccaccgc cagtttcatg gcctacgcgc tggggcaaat
    42001 cctcgacgtg cacgttttta accgcctgcg tcagagtcgc cgctggtggc tggcaccgac
    42061 agcgtccaca ctgttcggta acgtcagcga cacgctggcc tttttcttca ttgccttctg
    42121 gcgtagcccg gatgccttta tggctgaaca ctggatggaa atcgcgctgg tcgattactg
    42181 tttcaaagtg ttaatcagta tcgttttctt cctgccaatg tatggcgtat tactcaatat
    42241 gctgttgaaa agactggcag ataaatccga aatcaacgct ttgcaggcga gttaaaggtt
    42301 cgtaatcaga gttgtgataa gatggatgaa tgagccgtta tggccgttta tcgaaaggaa
    42361 gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc ctgctggtta tggggcttgc
    42421 ggcctgtgat gataaagaca ctaacgctac ggcgcagggt tcggtcgcgg aaagtaacgc
    42481 taccgggaat cccgtcaacc tgcttgatgg caagttaagt ttctcgctgc cagcggatat
    42541 gaccgaccag agcggtaagc tgggaacgca ggccaataac atgcatgtct ggtccgacgc
    42601 caccgggcag aaagcagtca tcgtcatcat gggcgatgat ccgaaagaag atctggcggt
    42661 gttggcgaag cgtctggaag atcagcaacg tagccgcgat ccgcagctgc aagtggtaac
    42721 caataaagcc attgagctga aaggtcacaa aatgcagcag ttagacagta ttatctccgc
    42781 gaaaggccag acggcgtact cttccgttat tctgggtaac gtgggtaatc aactgctgac
    42841 catgcaaatt acgctgccag ctgacgatca gcaaaaagcg caaaccaccg cagaaaacat
    42901 cattaatacg ctggttattc agtaagtttt aagatgatga ggcggcctca gggacgtgtt
    42961 ccggaggccg tttttttaat cgccacgtca gtaataacgc gattgcgacc agtcccgccg
    43021 ccgccagata aatcaccggt acgcccgccc agctcatcac cagcccagcc agtggtccag
    43081 tcacgccaag cgataaatcc ataaatacgg tgtaagttgc cagcgccgcc ccctaatttt
    43141 gctgcggaac cgcttttacc gccactacac ccaatgccgg gaacaccagc gaaaacccgg
    43201 cccccgccag taagacgccg attttcgcca tccacggcat agtcgccacg ccaaccagta
    43261 gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac gtttaagcca ccgatacggt
    43321 taatgccgtt agggaataac aaacgcgtac cgacaaacgc acagctaaac agcgtcagcg
    43381 cgaaagccgc accgtcccaa cctttagcgt cataaaacag cgtgataaag gtggcgatga
    43441 cgccaaatcc ggcggaagcc agtgccagcg ccataccgta cagccagacg cgcccaagca
    43501 ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac cgtcggacgc gggatcgcca
    43561 acaaaatggc caccagcgcc acgcccataa tgattaacgc taacgcctgc aagccgcccc
    43621 agtgataaaa cacgacgcct aacggcgcac ccatcgccat cgccccgtaa gtgacaatgc
    43681 cgttccacga aatcacccgc ccgatatgca gcgagccaac cactccaacg ccccataggg
    43741 tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat gacgcgcccc aggcaaagta
    43801 ataacaggct gatgacaggc agactggcgg ttaatcctgc cgtcagatac cccagaccgc
    43861 tcaaaaagca gccgcataaa ccgaagacga caatcttttt gggtcccagc aaatcggcgt
    43921 aacgtccggc atgagggcgg ctcagcaagg tggcgaaata ttgcaggctg ataaccaatc
    43981 ctgcccagaa ggcgctaaag cccatcacat catggacata gcccggtaat acagcgagcg
    44041 gcaacccgat ggtgaggtag ctggcgaagt taaacatgac aatggagaca atgcgcaaat
    44101 tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc gggcatgagg atcaccacat
    44161 ttttacaaca gtgtttcatt tttaccatgt gctgacgtga aaatcagcag taagaatcag
    44221 aatattgctg gcgtgactcc cgctacactt aacacaaaaa gtcacaagga agccccaatg
    44281 gaaacccctc aacccgataa aacgggcatg cacattctgc tcaagttggc ctcgctggta
    44341 gtgatcctcg cgggcattca cgcagcggca gatatcattg tgcagctgtt actggcgctg
    44401 ttttttgcca tcgtcctcaa cccgctcgtt acctggttta ttcgtcgggg agtacaacgc
    44461 cccgttgcca ttacgattgt agtggtggtg atgctgatcg cactaaccgc gctggtcggc
    44521 gtactggcgg catcgtttaa cgaatttatc tctatgctgc cgaagtttaa taaggagctg
    44581 acgcgcaaac tttttaaatt gcaggagatg ttgccttttc ttaatttgca tatgtcgccg
    44641 gagcgaatgc tgcagcggat ggactcggaa aaagtggtta ccttcaccac agcgctaatg
    44701 accgggcttt ccggggcaat ggcgagcgtg cttttgctgg tgatgaccgt agtttttatg
    44761 ctgtttgaag tgcgccacgt cccttacaaa atgcgttttg cgctgaataa cccacagatt
    44821 cacatcgcag gattacaccg cgcacttaaa ggcgtttcgc attatctggc gttaaagacg
    44881 ctactcagtt tatggacagg tgtcatcgtc tggctggggc tggcgctaat gggcgtacag
    44941 tttgcgctga tgtgggcagt actggcgttt ttgctcaact acgtgcccaa tatcggcgcg
    45001 gtaatttccg ccgtaccgcc aatgattcag gtgctgctgt ttaatggtgt ttacgaatgt
    45061 attctggtcg gcgcattgtt tttagtggtc catatggtca tcggcaatat tttagaacca
    45121 cggatgatgg gccatcgcct ggggatgtcc accatggtgg tatttctttc attgttaatc
    45181 tggggatggc tgctcggccc ggtagggatg ctactttcgg tgccattaac cagcgtgtgt
    45241 aaaatctgga tggaaacgac gaaaggtggc agcaaactgg cgattttact ggggccggac
    45301 agaccgaaaa gtcggttacc gggatgaggc gacaagtgat acgatacgca ctttcatttt
    45361 ccattaaacg ttggccctga tatgtatcgg atagttctgg ggaaagtttc gaccttaagc
    45421 gcagctccac tgccaccggg tttacgcgag caagcaccgc aaggtccacg acgcgaacgc
    45481 tggctggcgg ggcgtgcatt gctttcgcac acgctttccc cgctaccgga gatcatctat
    45541 ggcgaacaag gcaaacctgc attcgcgccg gaaacgccgc tatggttcaa cttaagccat
    45601 agcggtgacg atatcgctct gctgttgagc gatgaaggcg aagtcggctg cgatatcgaa
    45661 gtgattcgcc cgcgcgccaa ctggcgctgg ctggcgaacg ccgtgttcag cctcggggaa
    45721 cacgctgaaa tggacgccgt gcatcctgat cagcaactgg aaatgttctg gcgcatctgg
    45781 acgcgcaaag aagccatcgt taaacagcgt ggcggcagcg cctggcaaat cgtcagcgta
    45841 gatagcacct atcactcctc gctgtcagtc agccattgtc agctagaaaa tttaagcctt
    45901 gcgatctgca cccccacccc ctttacgctc accgccgaca gtgtgcaatg gatcgattca
    45961 gttaactgat ccgcccaccc gactgcccat ctattgatcc agaacaggta atcagtatga
    46021 cgaatactta aaatcgtcat acttatttcc gccatctatt ttaatccatt ggggttacca
    46081 tgctctccac actccgccgc actctatttg cgctgctggc ttgtgcgtct tttatcgtcc
    46141 atgccgctgc accagatgaa atcaccaccg cctggccggt gaatgtcggg ccactaaacc
    46201 cgcaccttta cacgcctaac cagatgttcg cccagagcat ggtttatgaa ccattggtga
    46261 aatatcaggc agacggttcg gtgatcccgt ggctggcaaa aagctggact cattcagaag
    46321 atggtaaaac ctggaccttc accctgcgtg atgacgtgaa attctccaac ggtgaaccgt
    46381 tcgatgccga ggcggcggca gaaaacttcc gcgcagtgct cgataaccgt caacgtcacg
    46441 cctggctgga gctggcaaac cagattgttg atgttaaagc actcaataaa acagagctgc
    46501 aaattaccct gaaaagcgcc tactatcctt tcctgcaaga actggccctg ccccgtcctt
    46561 tccgttttat cgctccctcg cagtttaaaa accatgaaac catgaacgga attaaagcgc
    46621 cgattggcac cggaccgtgg attttgcagg aatcgaaact gaatcagtac gatgtcttcg
    46681 tccgtaacga aaactactgg ggcgaaaagc cagcgattaa aaagatcacc tttaacgtca
    46741 tcccggaccc gactacccgc gcggtggcgt ttgaaactgg cgatatcgac ctgctgtacg
    46801 gaaacgaagg gttattaccg ctcgatacct tcgcccgctt tagccagaat ccggcttacc
    46861 acacccaact gtcacagccg ctcgaaaccg tgatgctggc gctcaatacc gccaaagccc
    46921 ccaccaacga gctggcagta cgtgaagctc ttaattacgc ggtaaacaaa aaatcgctga
    46981 ttgataacgc gttatatggc acccagcagg tcgccgacac cctgtttgcc ccttctgtgc
    47041 cttacgctaa tctcggcctg aaaccgcgcc agtacgatcc gcagaaagcg aaagagttgc
    47101 tggaaaaagc cggttggacg ctgcctgcgg gcaaagacat ccgcgagaaa aatggtcagc
    47161 cgctgcgcat tgaactgtcg ttcatcggca ccgatgcgtt aagcaaatcg atggcggaaa
    47221 tcattcaggc tgatatgcgc cagattggcg cagatgtctc gctgattggc gaagaagaga
    47281 gcagtatcta tgctcgtcag cgcgacggtc gttttggcat gattttccac cgcacctggg
    47341 gcgcgccata cgatccacac gccttcctga gttcaatgcg cgtaccgtcg cacgctgact
    47401 tccaggcaca gcaaggatta gccgacaagc cgctgattga taaagagatc ggcgaagtgc
    47461 tggcgaccca tgacgaaacg caacgtcagg cgctgtatcg cgacattctg acccgtctgc
    47521 atgacgaggc ggtttatctg cctatcagct acatctcgat gatggtggta tcaaaaccgg
    47581 agctgggtaa tatcccctac gcgccgatcg ccaccgaaat tccgttcgaa cagattaaac
    47641 cggtgaaacc ttaatgttgc gttacgtatt acgccgcttt ctgctgctga tcccgatggt
    47701 gctcgccgcc tcggtgatca tttttctgat gctgcgcctc ggtaccggcg acccggcgct
    47761 cgattatctg cgtttatcta acctgccgcc gacgccggag atgctggcct ctacccgcac
    47821 catgctcggg ctggatcagc cgctgtacgt ccagtacggt acctggttgt ggaaggcgct
    47881 gcatcttgac tttggtatct cattcgccag ccaacgcccg gtactggacg atatgctgaa
    47941 cttcctgccc gccacgctgg aacttgcagg tgcggcgctg gtattaattc tgctcacttc
    48001 cgtaccgctc ggtatctggg cggcgcgcca ccgcgatcgt ttgccggatt tcgccgtgcg
    48061 ctttatcgcg tttcttggcg tgtcgatgcc caacttctgg ctggcatttt tgctagtgat
    48121 ggcgttttcg gtgtatctgc aatggctacc cgcgatgggc tacggcagct ggcagcacat
    48181 cattttacct gcggtttcca tcgcctttat gtcgctggcg attaacgcgc gactgctgcg
    48241 cgccagtatg ctggacgtcg ccggtcagcg tcacgtcacc tgggcgcggc tacgcggcct
    48301 gaatgacaaa cagaccgaac gccgccatat tctgcgcaat gcctcgctgc cgatgatcac
    48361 cgccgtgggg atgcacattg gcgaattgat tggcgggacg atgattatcg aaaacatctt
    48421 tgcctggcct ggcgtcgggc gctatgcggt gtcggcgatt tttaaccgtg actatccggt
    48481 gattcagtgc tttacgctga tgatggtggt ggtttttgtg gtctgtaatt tgattgtcga
    48541 tttgctcaac gccgcgctgg acccgcgcat tcgtcgtcat gaaggagcgc acgcgtgaac
    48601 tttttcctct cttcccgctg gtcggtacgc ctggcgctga tcattatcgc cctgctggcg
    48661 ctgattgcgc tcaccagcca gtggtggctg ccgtacgatc cacaggcaat tgatttgccg
    48721 tcgtgcctgc tttcgccgga tgcgcagcac tggctgggca ccgatcactt aggtcgcgat
    48781 attttctcgc ggctgatggc agcgacccgc gtgtcgctcg gttcggtaat ggcctgcctg
    48841 ctgctggtgc tgatattagg gctggttatt ggcggcagcg ccgggttgat tggcgggcgc
    48901 gttgatcagg ccaccatgcg cgtcgccgat atgtttatga ccttcccgac ctcgattctg
    48961 tcgttcttta tggttggcgt gctcggcacc gggctgacca acgtaattat cgccatcgcc
    49021 ctgtcgcact gggcgtggta tgcacgcatg gtgcgcagct tggtgatttc actacgccaa
    49081 cgcgagtttg tgctggcgtc acggctttcc ggtgcgggcc atgtgcgggt gtttgtcgat
    49141 catctggcag gcgcggtgat cccttcgctg ctggtgctgg caacgctgga tatcggccat
    49201 atgatgctgc acgtcgcggg gatgtctttc cttggcctcg gtgtgaccgc gccgaccgcc
    49261 gaatggggcg tgatgattaa cgacgcgcgc cagtatatct ggacccagcc gctgcaaatg
    49321 ttctggccgg ggctggcgct gtttatcagc gtgatggcct ttaacctggt gggtgacgca
    49381 ctgcgcgatc atctggatcc tcatctggtg acggagcacg cacactaatg ccgcaacaga
    49441 ttgaactacg taatatcgcg ctacaggccg cgcagccgct ggtacacggt gtatcgttaa
    49501 ccctgcaacg cgggcgcgtg ctggcgttag tcggcggtag cggcagcggg aaatcattaa
    49561 cctgcgccgc gacgctgggc attttgcccg ctggcgttcg ccagacggcg ggggaaattt
    49621 tagccgatgg caaaccggtt tcgccttacg ccctgcgcgg catcaaaatt gccaccatca
    49681 tgcagaaccc gcgcagcgcc tttaatccac tgcacaccat gcacacccac gcgcgggaaa
    49741 cctgcctggc gttagggaaa cccgccgatg acgctacgct taccgctgcc atagaagcgg
    49801 tggggctgga aaacgccgcg cgcgtgctga agctgtaccc gttcgagatg agcggcggca
    49861 tgttgcagcg catgatgatt gcgatggcgg tgctgtgtga atcaccgttt atcatcgccg
    49921 atgaaccgac caccgacctc gacgtggtag cacaggcgcg catcctcgat ctgctggaaa
    49981 gcattatgca aaaacaagcg ccgggaatgc tgctggtcac ccatgatatg ggcgttgtgg
    50041 cgcgtctggc ggatgacgtg gcggtgatgt cacaaggtaa aattgtcgaa cagggcgatg
    50101 tagaaacgct gtttaacgcc cccaaacata cggtgacgcg cagcctggtt tccgctcatc
    50161 tcgccctcta cggtatggag ctggcatcat gaccttactt aacgtctgcg gcctttccca
    50221 tcactatgcc cacggtggat ttagcggaaa acatcaacat caggcggtgc tgaataacgt
    50281 ttccctgacc ctgaaaagcg gcgaaaccgt cgccctgctg ggacgcagcg gctgcggaaa
    50341 aagcaccctc gcgcggctgc tggtaggctt agagtctccc tcacaaggga atattagctg
    50401 gcgcggcgaa tcgctggcga aacttaatcg cgcccaacgt aaagccttcc gccgtgatat
    50461 tcagatggtg tttcaggact ccatcagtgc cgtgaatccg cgcaaaaccg tgcgcgagat
    50521 cctgcgtgaa ccgatgcgcc acctgctatc actgaaaaaa tccgaacaac tggcgcgcgc
    50581 cagcgaaatg ctgaaggcgg tcgatctcga tgacagcgtt ctcgacaaac gcccaccgca
    50641 gttaagcggc ggccagctcc agctcgtctg cctggcacgc gcgctggcgg tcgaaccgaa
    50701 attgctgatt ctggatgaag ccgtttctaa ccttgatctc gtgttacagg cgggcgtcat
    50761 tcgcctgctg aaaaatctac aacaacagtt tggcactgcc tgcctgttca tcactcatga
    50821 cttacgcctg gtggaacgct tttgccagcg agtgatggtg atggacaacg gacaaatcgc
    50881 cgaaacccag gcggtgggag ataaattaac cttttcctct gacgccggac gtgtgctaca
    50941 aaacgcggta ttacccgcat tccccgtgcg ccgtcgcacc tcagaaaagg tttaacgcca
    51001 atgcaacgag tcaccatcac gcttgatgac gatttactgg agacgctgga cagcctgagc
    51061 cagcgtcgtg gttataacaa ccgttccgaa gctatccgcg atattctgcg tagtgccctg
    51121 gcgcaggaag ctacccagca gcacggcaca caaggtttcg cggtgctgtc gtatgtgtat
    51181 gaacacgaaa aacgcgactt agccagccgt attgtttcca cccagcatca tcaccacgac
    51241 ctctccgtcg ccacgctaca tgtgcacatc aaccacgacg actgtctgga aatcgccgtg
    51301 ttgaaaggtg acatgggtga cgtgcagcat tttgccgatg acgttatcgc ccagcgcggc
    51361 gtgcggcacg ggcatttgca gtgcctgccg aaggaagatt gagttatttc taaaacaagg
    51421 aaagaaaaga tattaactga acttaattta taaaattaag ttcagttgca tagatagatc
    51481 ctgtaaaggc agatattctg cgtaatgtac gtcgctaaaa tttataaatg attcaaacgc
    51541 tcattcagtg tttcaataat ataggtatta acagagactt gttgctgcgc agccgcatta
    51601 ttaaggcgtt cactcagcga ttctggatag cgtaacgtaa aggtcttaat cttttctgta
    51661 cgcgcatacg gctcaatacc cgcagcttta caatcctcaa gatactctcg cagggaaagc
    51721 tctccttctt tttgcagccc ctgaatactg tcagagacaa aatcacaata acctgacaag
    51781 cccaggaatt tgccacgaaa tgcattcagt tctggcacgt aggtaataac agctggttgc
    51841 ccggcaatct ccatagagtt tggcgtcttc agtttgatca tggttttacc cctatactta
    51901 gtaaccagtc acgcacgctt tctacagcgc ctttatcggt atctggcgac ggatgaggcc
    51961 gatggaaaca cgcaacgctc atatttagta tgaacttgca acgcgaacct cttccttcct
    52021 taatttctcc gcctaatgct ttaaccagtg actctatatc agaccacttg atcccttgag
    52081 gaacaggtgt tttaaatatt tgatccaatg tatttttttg tttcttccgt aaagcctgta
    52141 ccttggttct catggcatgt ccttatgact tcactttatg acatcacagt atgaagtcaa
    52201 gtttcccttc cttacgccat tgtcccaatc gtcttcctga atcgcagcag tgcgatggtg
    52261 aaaaacgcgc cgccaattgc catcagcgtc aggaactgcg gccagacgat ttcgaatccg
    52321 gcaccccggt agaggatggc ctgcgcgagg ctaacaaagt gtgtcgtcgg catggtcagc
    52381 ataatgtcct gcaccatctg cggcatactt tcgcgcggcg tggatccacc ggaaagcatt
    52441 tgcagcggca gcagcaccag aatcaccagc agccccagtt gcggcattga acgcgctatc
    52501 gtccccataa aaatgccgat tgacgtggtg gcaaacagac tgagcgccac gcccagcata
    52561 aacagcggga tcgagccttc aatcggtacg cccagtacac ctttcaccat cagcaccagc
    52621 gataatcccg ataccaccag caccaccagc cccatcgacc agatcttcgc catcatgatc
    52681 tcaaacggcg ttatcggcat caccagtaaa tgttccaccg tgccgtgttc acgctcgcgg
    52741 atcagcgccg atccggtcaa tacaatcgcc agcatggtaa tgttgttgat gatcgccatc
    52801 accccgccaa accacgcggg atcaaggttc gggttaaagc gcatccgggt ttccagcgat
    52861 accaacggtt cgctgttatc acggtagcgc gcgacaaagc tgttcacttc accgttgata
    52921 atattctgga tatacccatt gccggtaaat gcctggctca tgcgcgtggc atcgacgttc
    52981 acctgaatat ccggctggcg tccggcgagg acatcacgct gaaaattagg cggaatattt
    53041 atcgcgaagg tatagcgtcc ggcgtccagt ccggcatcca tctcatcggc ggtgatcatc
    53101 tccggtggca aaaaccacgg acgatagaag ctgttaacga tccggttcga taactgcgat
    53161 tgatccatat cggcaatggc gatcggcgcg aggttcaacg atcctggcgt aacggtcgct
    53221 gacgaataca ccgacaccgt aaacgagaag acaatcagcg tcagcatcgc tttatcaccg
    53281 agcagactgc gcaactcttt gatacccaga ttaaaaatat tgcgtaactg gcgcatcatc
    53341 cctcctgttt tttcagcagc aggatgctta agcccatcac cagcgggatg gctatcagca
    53401 acgggataaa aagttgccac aaatcagtca gatccagcgc tttcgagaac gttccgcggg
    53461 cgatagtcag aaaatgactg gtcgggtaaa cctcgccgat ccaacgtcca ggcccttcca
    53521 gcgaagctac cggatcgatc atcccggaaa actgtgtcgc cgggatcaat gtgataatcg
    53581 ccgttccgaa aatggcggca atctggcttt tcataaaggt ggagatcagc agccccattc
    53641 cagtggcaat gatgatatac agcagcgccg ccaaggtgag cgtcaggaaa ctgcctttat
    53701 gcggtacgcc aaacacaaac accgacaggc cgcagagcag gaaaaagttc agcatcccca
    53761 gcgcgatgta cggcagctgt ttgccaagca aaaattcgct acgggtggtg ggggtcacgt
    53821 aaaggttgat aatcgaccct agctcttttt cccgcaccac gctaagggcg cttagcattg
    53881 acgggatcat catcagcaga agcgggatca ccgccggaac aatcgctggc aggcttttta
    53941 cgtccgggtt atagcgataa cgcgtctcaa tgttcatcag cccgctttgg ctggcgggtg
    54001 tcgattgtcg gctcgccaca tcctgaagcc aactctggtg cattgcctgc acgtaacctt
    54061 ttaccgtttc ggcacgactg ggcatcgctc cgtcgatcca gacgccgagt tccacaggcg
    54121 taccacgcgc gatatcgcgc ccgaaattgg gcgggatctc aatggccacc gtgatatcgc
    54181 ccgcacgcat ccgacgatca agctcgtcat aactggtgag cggcggctgt tcgataaagt
    54241 aacgggaacc ggagaggttg agtgtccacg cctggctact gacggtctgg tcgcggtcga
    54301 gcaccgcaaa gcgcaggttt tccacatcca tactgatgcc gtaacccatt atcagcatca
    54361 ggatcaccgt tcccatcagc gccagcgtcg aacgtactgg atcgcgtcgc agttccagcg
    54421 cttcgcggcg gctgtagcta aacagacggc gcaggctaaa tccctgacgc ggcgcgtggg
    54481 tggtgtcgtg taccacgggc ggcgcttcgg cttcgttgct ctgccctgcc gcttcctgca
    54541 aataggcgat aaatgcctct tccagactgg cggctccgcg tttctcaacc agttcctgcg
    54601 gtgtaccgct ggcaagcact tttccggcgt gcatcagtga gatgcggtcg caacgttccg
    54661 cttcgttcat aaagtgggtg gagatgaaga tagtcacttt gtcctggcgc gagagatcga
    54721 ccatcaactg ccagaacata tccctcgcca ccggatcgac accagaagta ggctcatcga
    54781 ggattaacat ctccgggcga tgaatcaccg ccaccgccag cgaaagccgc tggcgaatgc
    54841 cgagcggcaa tgactccggc agaacatctt caacgtcgtt gagcttaaaa cgctcgctca
    54901 tttcagccac tcgcgcggga atttccgctt ccgggatgtg aaacaaacgg gcatgtaact
    54961 ccaggttttg ccgcacggtg agttcgttat agagcgaaaa cgcctgcgac atatagccca
    55021 cacgacggcg ggtatcgata tcttttggat caaccggttg cccaaatagc cacgcctcac
    55081 cttcgctggc gggcagcagt ccggtaagca ttttcatggt ggtggattta ccgcagccgt
    55141 tcgaaccaag gaatccaaaa atctccccgc gtggaatgcg gaaattgacg tgatcaacgg
    55201 caacgaagga accaaaacgc atggtcagat cgcgcgcttc gatggcaatc tctgcgtttt
    55261 caggttgata cggtgggatc actaccgcct gatgcgcctg gcgttgcgct tgcggtaaca
    55321 gattaataaa tgcttcttcc agcgtggcgc tttgcgtttg ctgccgcagc tcttcggcgc
    55381 tgccggttgc cagcacttct ccggcattca tcgctaccag ccagtcgaag cgttcggcct
    55441 cttccatata ggcggtggcg accagcacgc tcatattgct ctgccgctgg cgaatactgt
    55501 cgatcagatc ccagaactgg gcgcgggaga gcgggtcaac ccccgttgtt ggctcatcaa
    55561 ggatcaacag ttccgggtcg tggattaacg cgcagcacag cccaagtttt tgcttcatcc
    55621 cgccggagag tttacctgcc ggacgatcgc gaaacggtgc taacccggtg ctggtcagca
    55681 gctcattaat tcgcacttcc cgctccgctt tatcgtgacc gaacaggcga gcgaaaaaat
    55741 cgacattttc atacaccgac aaggtgtggt agaggttttt gcccagcccc tgcggcatcc
    55801 aggcgatgcg cgggcagacg tctcggcgat gcttcgggtc gtgcatatcg ccacccagca
    55861 ccaccacatt gccctgctca atgacgcggg caccggaaat cagtgacaat aaactcgact
    55921 tcccgacgcc atccggacca atcagcccga ccatgcaacg ggccggaata tcgagagtga
    55981 tattgttcag cgcaacggtt tttccataat gctggctcac gcccgccagt tgcgcgacag
    56041 gcgggacagg aaccagttcc agatgcgtca ttgcggcaac ctcaccacga ggtcgtcagg
    56101 ccacggaagt tcttcattca cccgcaccca cgctacgccc ggtaaaccgg ttttgacata
    56161 ttccagatgc tgctggagta attccggtgg gatacgcgct ttgacgcgga acatcagttt
    56221 cagccgttca tcgctggttt cgacggtttt tggcgtgaac tgggcgacac tggcgacaaa
    56281 actgatggtt gcaggaatac gcagatctgg cgcggcatcg aggatcagcc gggcttcacc
    56341 gcccagtttc agcgtgcccg cctgttcggt tggcaggaag aaggtcatat agacgtcgct
    56401 gagatcgacc atattcagca cccgaccgcc tgccgccagc acttcccctg gctcggcaac
    56461 ccgatactgc acgcgtccgt cacgcggggc tttcagttca ctgtcatcga tatctgcggc
    56521 aatgcgccgt tcagtggctt gcgctgcttc gacccgagtt tgcgcctgaa tgatattggt
    56581 gcgtgctgct tctatagccg ctttagaagc cgaaacctgg gctttcgctg attccagcgc
    56641 agctcgggcg ctctcagcgg cggcgcggtc gtcatccagc tgttgcgcag aaatagcccc
    56701 tcgttgggcg agcgagcggg aacgcgtatg acgtttggca acagagtcca gttcggcctg
    56761 gcgttgattg accagcgact gtgcggcacg agtttcactt tgtcgttgct ccagcaaagc
    56821 ctgcgcggcg gcaacggcgc tttgtgcctc tttgatttgc gcgatggctt ccagtcgctg
    56881 ttcctgcaac acgcgagtat ccatcttcgc cagcacttcg ccttcgcgaa caaactggcc
    56941 ttctttcacc agaatggtgt cgatacgccc ggcagttttg ctggcgatat ccacttccgt
    57001 cgcttcaatg cgcccattgc tgacagcaaa gccttccggc acacccgccg ggcgcaacag
    57061 ccaccacgcc acgacggcag ccaccgccag tgccccgaca acccaccacg ccagatggcg
    57121 cttactctta tccataatcg acccgccata atccctgtaa gcaaacgacg cgtgttacaa
    57181 ctgccgccga acgacgttca gcggattctt ccatccggga taagaaggaa acgacaaata
    57241 cgaccagcgc gcagagcatt gagcgccaac ggggaaggct tagcgaaaac gggtagtaat
    57301 gttgtcatca tcagcattcc tggccgtaaa tgaaacacgc atactcataa gtgtgatcta
    57361 taaaactggc tgacaaaacg gtatccatcc tgttggatcg cgtttcacgc ctgttcagtt
    57421 gcaggaacag agcattcctt tttacgtttt atcaacagtc tcaaccagat agtaacatta
    57481 atattcatgt tgtctatggt tcagagctgt aacattgtgc gccttctaat caaaggcata
    57541 agtccatttc tgtttttttc atgctggtaa gacttccagg gagtggttat ctctggccct
    57601 gcaccagggt taatttggca attatgcatg ctgaacattc ttccagcatg tttactgtca
    57661 cgggcgttaa gacctaaaac tgtccccgtt tcacctatgc gttctctccc accatttccg
    57721 ttatgtgcaa caacttgatt tatacctaac atttgtgaac atgctaatag tgcttcaagt
    57781 ttaggtctgt aatctgtcga aacagaaaaa taacgattaa aggatcgaca agagatagcc
    57841 cggctagttt taaacttttt gccattaatt atagcatgat gctttttatt aataatttcc
    57901 gctaattcga ccggatcgag agcattggtc atttcatcaa tagagacagt tatcggtcct
    57961 ataatgtaac gttttcgctg atcatcaaaa gttatcccat gatgatttgc cattattttg
    58021 tatttggggt catagaaaca tacaggataa tcttttatcc cgtcgtaggt atcctcatca
    58081 agtggtttca tgcgcgtgaa gttttgcata tagttaccat tgcaattcgt ttcatgattt
    58141 ccagctaata catgaacatt tttattgata ctcattatgc gttttaacaa atcgatgata
    58201 aattgaacgc cacagatatt actaaatcga tcgccggttt ggtcgccaag aaacgtacat
    58261 ggcgtgactg cgtctttatt aataaccaaa taaggctgaa gactttctaa aacattatct
    58321 cttctgttca gtatactgat tattgtatta acattagtgt caagttgctc attaaagttc
    58381 tcccttagca atacgttgag aacttcatct tctatattaa gcaactggct aaggaaattt
    58441 tctccttcag ccatgattcc caaatgtcct gttgcaaaaa gcgcgtacaa aactgcaccg
    58501 actgagctat cggtatcgcc aaaataggtc ggcctggtac ttgtgggatc aacaggtgaa
    58561 caattggaga aataggttct gtcagcgaac tggggtttta taattgtttc gaatttaata
    58621 tcaaatacat tgtattgcga tatttcccag ttccagatgg gtttgtacga ctcagtaact
    58681 gcaacatcat tagcattatc atttactgtc atattattaa gtaaataaac aatgctgcac
    58741 caattaatat cggtattatc aagagggcaa ccgtgatttt tactattaat tgtcacaata
    58801 aactgataac agttattcct acgtagaata tgagcgcgca tgcccgatag catactatat
    58861 ttttctgaat gattcgtttg tatggtaaat gaaattttgt catcttgaat agattgttgg
    58921 aaacgttcta tttgccaatc atcaattttt tccacaactt caccgagcat tgtcctggca
    58981 atacttgata tatcatcacc tataccattt gcaatatacc caaacagttc tttagtgaag
    59041 ttggtaacat gctcttgcac catatcattt gttgcgattg tcgttgtact gccattagtg
    59101 cctgccacag ttccaatttt catcttttac ctcatattat tcaccgcgag gaatacatag
    59161 ttaacatttt atcttatgac atacacctca tattcgatgg gaaaataatt atattttgca
    59221 caaatcattc agccttaaag cataatatga aatttcttat ctcatagcag agaaagtccc
    59281 taaagattgt agaggcgtca tcagtacata cctctacaat cgattaagaa aacctatgaa
    59341 atactggcaa aattaaaaag agataccaca gcactcatga agttttattg aggttgaaaa
    59401 caactaagcc ctgggacacc ttttttatca ccacgttcgt taggtgctat cttccaagaa
    59461 tttagcccca tggtataatt atcaattttt tgatgctggt ctacccctaa tccataatgg
    59521 ccaaatttct gtttagtttt tttgagcgtc tgtccacatt tgaagatatc ttcaggtttt
    59581 ggtctgaaag cagtagtgtt attaaaataa ttttcatata caggtgtggg tgctatagca
    59641 ttgcttctaa ttagatggag aagatgatca tccatgtgtt gtttatgttt tttattaaat
    59701 atatttgcta attcctcagg atcaattggg tctttcatct gattaaatgg aacctgcaaa
    59761 gcaccgagac aataacattg actttcttca tttcttatta ttccgtgatg actggttaaa
    59821 actttagttt ctgagttata attacaaaca tcgagcgttt taattaagtt atacgtatca
    59881 ccggcagaga gtttataatt ttctagtcgt gcagtatagt taccattaaa attgatttca
    59941 tgatttccag ccagaacgat gacattttta ttaatacggc tatctttatt accctccata
    60001 tttcgcatag aatttagcaa agtaaggata tatttatcac caaaaatagt actaaatcta
    60061 tcgcctgtat gatccccaag ggggatacaa ggggttatcg catcgtcctt taaaaaaaca
    60121 tgttgatgca actcattcaa tatcatgtct ctcgtattta ataaattgta gagcaattta
    60181 ttatttttat gtatgtatgc agatgaagcc atgtcttcgt gttttaataa ttcacataat
    60241 aaactccaac ccttttccct gatgcctata tgccctgaaa cgagcaatga ataaagtaca
    60301 gcgcctactc ttccatctgt atcgccaaaa taagccgtgt tggcattttg tgggttaatc
    60361 acaccacatt gtttataaaa gtctatttta gaaaacacag atttaacata gctatcaaac
    60421 ttatccttag gaaacgtctc tttagagaca tcccaacgcc agtcagcttt aaaattaccg
    60481 ctaagaagag taaagaaatt ttcaggtatg atagaaagcc cattaacttt ataaattatg
    60541 tctgatttat ctccggagaa attaataaaa taataaccat ccttacactc aatcttaact
    60601 gtaatgggag acgataattt tatcacatgg ttattgtctg gtataattgt aaagataaac
    60661 gtgttagtag tattatcggc aaaactatta tattcttgaa tatctataga tggctcacta
    60721 ttgtaaaacc atattttctc catctgaaga ctatgttcat ttaccctcca gtgaccatgt
    60781 tgcaggaaat agatgatatg catgccaaaa tcgttatcta tttgcgaatc attatccagt
    60841 ttatcaattt caactatctt tgaacgatgt gttttatcaa ccttgctatg gtgttgatga
    60901 ttattaactt tatctgaatg cttatcttta tggtttgaag ggatgtactt aaatatattg
    60961 tcaattgctt tcataaataa cccgcgatat ttttgacctg ttagtaaaaa ataaatgata
    61021 catgtctgaa agacatattt acgacgatat aaaacaataa tgcttatatg ttcgctgatt
    61081 ttaagcaccg agactatccg gtcttttact aaataatgga aaacaatcca tccctggaag
    61141 acaattgtaa aaaaaacaac agaataagaa ttaatcttag gataaatatt tatttatcat
    61201 ggccttttga ccgctaaaaa atggcactcg gcacagtaaa ccccgctata gttatgctat
    61261 acatttgtct ttaatgatat ctcgtcgtta tattaacagg ctgaaattat cattaatgca
    61321 catttcaata ttagcaggga tacctgagga gtttaaagtg aacatatata tcgggtagct
    61381 tttcaaatta atccctttga ttataggctt aatttgcatc gcgttaggtg gctttgtgct
    61441 ggaaagttca gggcaacgtc acgggtcatg tgctgatttc tctggcggcc atatgcctgg
    61501 cattattcac taccgcattt attatcattt cgcagctcac gcgcggcgtt aatacgtttt
    61561 acaatacatt gttccccatt attggctatg cggggtcaat tatcaccatg atatggggtt
    61621 gggcactgtt agcaggtaat gatgtgatgg cagacgagtt tgttgccggc catgttattt
    61681 ttggcgttgg tatgattgcc gcatgtgtat cgacggtggc agcgtcgtcc ggtcactttc
    61741 tgctcattcc caaaaatgca gcggggagca agagcgacgg aacaccggta caggcttatt
    61801 cttcattaat cggtaattgc ctcattgccg ttcccgtttt actcaccctg ctcggtttca
    61861 tttggtctat tacgctgtta catagtgccg acataactcc gcattatgtc gccttgggtt
    61921 aaccgcaatc tgtgcctgtc taattggcct agtggccaca atcgtccatc aaacacgtaa
    61981 tacgttctca tccaaagagc actggctgtg gtgttattgg gttatttttc tcggctcagt
    62041 cacggtgctg caggggatat acgtcttagt cagttccgat gcaagcgccc gactggctcc
    62101 cggcattatt ctcatttgcc tcggaatgat ctgttacagc atattctcaa aagtctggct
    62161 actggcactg gtctggagac gtacctgttc gttagccaac agaataccga tgataccggt
    62221 atttacctgt ctgttttgct tgtttttgtc atcattcctc gcggaaatcg cacaaactga
    62281 gctggactac tttattccat ctcgggttct ggttggatta agcgcagtat gttttacatt
    62341 gttctctatt gtttcaatat tagaggccgg ttctgcgaaa aaaaattaaa atttacataa
    62401 tcccagcgtt attcatatct taacgctggt tttttacgaa cttagtgaaa taatgtagaa
    62461 tataaaatta taattttata ttggagcaca tgtgttaatg atcaaccccg ggatactaaa
    62521 aatatagaca ttattaaact tgcttagcct cgcattgcct ttacctgtta aagatgcatc
    62581 gagaaaattg agtctgaagt tctcatcctt tatctgattt tccagtcccg aaactttggc
    62641 cttagcatta cctgagatcc gtccgttact atctattttg taggtaaaaa aatacgaatg
    62701 ggaaaatttc ctcggtgtat cttcatcagt taagccaaca atcgtaaaac taccgctacc
    62761 atcttttttt aaaatgactg aaaggttagc agagtagtat aaagaatgat ctgttttagc
    62821 atttcttgtt aacgtcgacg tacattcaag agacgttgtg ctaccatcac gagccttcca
    62881 gagagcaaaa aaacacacct gtagcaaaaa tacattttct tattagtcat ataatagcac
    62941 ctctgaacaa attgcataag tttcgccatt agaggatttt atacatcttg ataaataccg
    63001 ctgcccttcc ccaagcaaat aatgcatact atttacatca tatataaaat ttgcacctgg
    63061 cacacagggt aaatgctttt tcgaaatttc agcggcctct atttgagatt tagcgaggcc
    63121 aatctcgtta agattcgctg aaagatataa atcacaggtg tcaatcttac ctgatttaac
    63181 caatggataa tccgttatac tgtaggtatc tttaaaagcc acggcaaaca tttcaataat
    63241 aacaattact accacaagcg aaatcaaaat gcgaaaaaga ggaatactcc ttttgcgctc
    63301 tactttttca acaacatctt cttgtaaatg agaatcatca taagattgtt tacttattgt
    63361 tatgttttca ctgatgtaaa agcctacttt gggtatcgta agaatcacat tttcttcaac
    63421 accaatattt ttaaaacttt tgcgtaacag ggatatatat tggttcaatg tgttgtttga
    63481 cagatcaaat gaaaatctct ccaaaacgct atcaatgatt gagttcctgt caaccgctcc
    63541 accattactt tccagtaaca ctgccaaaat acaccccgcc gtagctggca aatttatctc
    63601 atcctgggta tttacattcg agagcgagca tttgttcgga tcaaaaataa tcgcgtcatt
    63661 gataatgaaa aaacatctcc gctccatgca ggcaacaaca caaaataaga ttttgtcatt
    63721 ttagcctatt cctggtggaa ctgacacaag aaactaaaat acaaatataa ccctgaaaaa
    63781 gtcatataaa acttatatcc acagaataaa aaaacaaaac atgtcagttt tatcaatata
    63841 aagtatcgca aggcgaaaag tgatgcaaga atactcctaa gtaaatgaaa gcgcaccaca
    63901 tgaaattatc aacacatgta atttcatcat taggtgctat cgaaaataac tcaggagcta
    63961 cttaagatga ataatgtctt actcatgaga cccgatgact cagaatttat gatacctgat
    64021 gactggtcag ggctaaacgc aaaaggcatt acatacgaac aagataatag agtgacagag
    64081 tcttttctgc cttttaaatc tttttattgt tcaccggtgt gcacgacagt tgaactcatc
    64141 tcacaaaata attgttttgc tatgggtatc cgctaccttc tggaaaagaa aagagactta
    64201 aggctaaatc actttaaagc cttctctcat aaatgcgaga caaaacctga cgtgttaatt
    64261 atgaccgggt gcaccgtcgc tgatacttat agtatattct cacgcattgt tgaagaaaat
    64321 ataatgaaaa cgatgacacc tatcgtcatc attactgggc atcactcaga agatctttgt
    64381 tctctggcat cgaaactggg aggagttaaa ataatatcac cgacagaaaa gattgcaaaa
    64441 atagaaaacg ctatcaatga tgcttttaac agcaagccta aaaaaataag atttttaaca
    64501 gatatactct ctcttcaaca aattgagacc atgaaaatgg ttgcgcaggg tagatccgtt
    64561 aatgagattt cagccgcaat gaatattagc atcaagaccg tgtatgcaca taaagttcgt
    64621 gctatcaata agctgggaat taagaaaaaa gtttatgaag cgctttttta taaagcgcta
    64681 attcagttca actgatgtaa aaatcaccct cgcttaataa ataatatatt ctatgtcttt
    64741 ttttataagt taaaaaaggc gggatatcaa atcccgccat gaaattaact aatcatctgc
    64801 attattctgc gtagatataa gcctgggtac ggatggtgta agagtctggc tcaacaacct
    64861 gttctttcat atgggaaacg acgcgatagg caccacctgt tccagtattt ttcagcatca
    64921 tataacgctt accgccaatc tcttcggagg cagctgaagg aggatcctgc tcaagcgttg
    64981 tcagggaaac tttcagttta tggtccgggt tattgtcacc agaaattact gcatccgctg
    65041 aagtactgct ctccgcataa atatcatccg ggaaagttac agcatagtcg tggctctgcc
    65101 catcagatgc gccaacaata cctgttgcca aaggcgtcca tgcagttaac gtaccgctat
    65161 ataccccttt cactggttga atggtattat taacaacgat aggcttggtc aaagtaagat
    65221 tagctgacgc ttcagtaccc tggcttacta aagttcccgc ctgagaagca aaagctgtcc
    65281 ccgccagcat gattgctaac aatgttttct tcattatatt ttctccatga ttttcattta
    65341 cactgcaaaa tgcagtttct ttctttgcat ttacgtgcta tcgcgaaaat gcttccacca
    65401 aataatggaa acatcttcac aaaaataaat tatgagtttc ttaatttaga aactataaac
    65461 atgtcgccgc tatctggtat aaccctgatg cacttttctt cgcaggaagt tggtattttg
    65521 cgatacagga ttgatcttta gaataacccc atttcacatt aaactcgcct tccgcactca
    65581 agccagaaac ataagcaata ccatcttcac ctacaatgcc ggccatattt tcagtgtctt
    65641 tgctataact aatcacagcg ccgaagggga cgggtttacc ctctttctgt tttaaggtta
    65701 ctaatccttt atccccgctc ttcactgcca atgtcgcagg cacaacggct ccagctgtcg
    65761 gtacaaccaa ttgactagtt tgtggtaagt cgtaattctt acctatcgtt ttttcatcca
    65821 gaataacatc gtctcttcgg tatggctgca ctggcagtat tgcataccca cgaaaatcag
    65881 tactcagtcc cggccagcca ttaacgttaa cccccgttgc tccgggtgcc tctataaggg
    65941 ctaatgtccc atccacattt tgaccaaatg taagtccatc ccttgtaata agtatcccac
    66001 cgcttaaatt tgcacctagc tgtttcatgt tttcgctgga gctgtaatgg ctactaaact
    66061 gaccatatga tccatttttg ccaacttgca caatgttcat cgtgctcttc ggttgtgact
    66121 gatgagcgtt agttatattt gtcgctacat cccaccatac accaccacta ttctgacctg
    66181 ataatgaaac ctgatagtta ttaccctgct ctcccgaacg actcatctgt gaagacgcat
    66241 aaatttgatt ttcgtcaccc agcgataatg gcactgaaat acccacgctt aatttctgat
    66301 ctactttcca tttatgcgat gtcagcaagt cgttttttgc ccagttaatg gagaatgtta
    66361 tgcctctcca taccgtacta taactcaggc tggtagaccg cgtgtttcct gtgccccggg
    66421 aagaattata cctcgtggta ttgaagttca aattcccgat attaccaagg ggctgggata
    66481 aggatatcgt agagccatcg tgactattct tatctctttt tctgttccgt tcatcctgtg
    66541 cacttactgc ttcagtcagt gtggcataac ctgatgcata acggttcgtg gttatagac
//
LOCUS       DADMGC010000006        65613 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.006, whole genome shotgun sequence.
ACCESSION   DADMGC010000006 DADMGC010000000
VERSION     DADMGC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.006"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(129..608)
                     /gene="folK"
                     /locus_tag="JNZ73_000393"
     CDS             complement(129..608)
                     /gene="folK"
                     /locus_tag="JNZ73_000393"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAY8730801.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="JNZ73_000394"
     CDS             complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="JNZ73_000394"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HAY8730802.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="JNZ73_000395"
     CDS             complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="JNZ73_000395"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706092.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HAY8730803.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQGR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3025..3480)
                     /locus_tag="JNZ73_000396"
     CDS             complement(3025..3480)
                     /locus_tag="JNZ73_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HAY8730804.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="JNZ73_000397"
     CDS             complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="JNZ73_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAY8730805.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="JNZ73_000398"
     CDS             complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="JNZ73_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HAY8730806.1"
                     /translation="MSEPQRLFFAIDLPAETREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4981..7410
                     /gene="hrpB"
                     /locus_tag="JNZ73_000399"
     CDS             4981..7410
                     /gene="hrpB"
                     /locus_tag="JNZ73_000399"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HAY8730807.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPNDPAN
                     TAPTRRTKKYS"
     gene            7612..10146
                     /gene="mrcB"
                     /locus_tag="JNZ73_000400"
     CDS             7612..10146
                     /gene="mrcB"
                     /locus_tag="JNZ73_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HAY8730808.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLAAIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10366..12609
                     /gene="fhuA"
                     /locus_tag="JNZ73_000401"
     CDS             10366..12609
                     /gene="fhuA"
                     /locus_tag="JNZ73_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HAY8730809.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FGENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12660..13457
                     /gene="fhuC"
                     /locus_tag="JNZ73_000402"
     CDS             12660..13457
                     /gene="fhuC"
                     /locus_tag="JNZ73_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HAY8730810.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13457..14347
                     /gene="fhuD"
                     /locus_tag="JNZ73_000403"
     CDS             13457..14347
                     /gene="fhuD"
                     /locus_tag="JNZ73_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HAY8730811.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14344..16326
                     /gene="fhuB"
                     /locus_tag="JNZ73_000404"
     CDS             14344..16326
                     /gene="fhuB"
                     /locus_tag="JNZ73_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HAY8730812.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVMLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16361..17641)
                     /gene="hemL"
                     /locus_tag="JNZ73_000405"
     CDS             complement(16361..17641)
                     /gene="hemL"
                     /locus_tag="JNZ73_000405"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HAY8730813.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLMTEL
                     VPTIDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17866..19287
                     /gene="clcA"
                     /locus_tag="JNZ73_000406"
     CDS             17866..19287
                     /gene="clcA"
                     /locus_tag="JNZ73_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HAY8730814.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LIGLAAVAFDKGVAWLQNQRMGVLVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19311..19376
                     /locus_tag="JNZ73_000407"
     CDS             19311..19376
                     /locus_tag="JNZ73_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8730815.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19369..19713
                     /gene="erpA"
                     /locus_tag="JNZ73_000408"
     CDS             19369..19713
                     /gene="erpA"
                     /locus_tag="JNZ73_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HAY8730816.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19760..20383)
                     /locus_tag="JNZ73_000409"
     CDS             complement(19760..20383)
                     /locus_tag="JNZ73_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HAY8730817.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20421..21221)
                     /gene="btuF"
                     /locus_tag="JNZ73_000410"
     CDS             complement(20421..21221)
                     /gene="btuF"
                     /locus_tag="JNZ73_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HAY8730818.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21214..21912)
                     /gene="mtnN"
                     /locus_tag="JNZ73_000411"
     CDS             complement(21214..21912)
                     /gene="mtnN"
                     /locus_tag="JNZ73_000411"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAY8730819.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPTGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21996..23513
                     /gene="dgt"
                     /locus_tag="JNZ73_000412"
     CDS             21996..23513
                     /gene="dgt"
                     /locus_tag="JNZ73_000412"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAY8730820.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23643..25067
                     /gene="degP"
                     /locus_tag="JNZ73_000413"
     CDS             23643..25067
                     /gene="degP"
                     /locus_tag="JNZ73_000413"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HAY8730821.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25222..26378
                     /locus_tag="JNZ73_000414"
                     /pseudo
     CDS             25222..26378
                     /locus_tag="JNZ73_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
     gene            complement(26467..26853)
                     /locus_tag="JNZ73_000415"
     CDS             complement(26467..26853)
                     /locus_tag="JNZ73_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HAY8730822.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27167..27991)
                     /gene="dapD"
                     /locus_tag="JNZ73_000416"
     CDS             complement(27167..27991)
                     /gene="dapD"
                     /locus_tag="JNZ73_000416"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HAY8730823.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28022..30694)
                     /gene="glnD"
                     /locus_tag="JNZ73_000417"
     CDS             complement(28022..30694)
                     /gene="glnD"
                     /locus_tag="JNZ73_000417"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HAY8730824.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30756..31550)
                     /gene="map"
                     /locus_tag="JNZ73_000418"
     CDS             complement(30756..31550)
                     /gene="map"
                     /locus_tag="JNZ73_000418"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAY8730825.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31918..32643
                     /gene="rpsB"
                     /locus_tag="JNZ73_000419"
     CDS             31918..32643
                     /gene="rpsB"
                     /locus_tag="JNZ73_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAY8730826.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32901..33752
                     /gene="tsf"
                     /locus_tag="JNZ73_000420"
     CDS             32901..33752
                     /gene="tsf"
                     /locus_tag="JNZ73_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAY8730827.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33899..34624
                     /gene="pyrH"
                     /locus_tag="JNZ73_000421"
     CDS             33899..34624
                     /gene="pyrH"
                     /locus_tag="JNZ73_000421"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAY8730828.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34916..35473
                     /gene="frr"
                     /locus_tag="JNZ73_000422"
     CDS             34916..35473
                     /gene="frr"
                     /locus_tag="JNZ73_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAY8730829.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35565..36761
                     /gene="ispC"
                     /locus_tag="JNZ73_000423"
     CDS             35565..36761
                     /gene="ispC"
                     /locus_tag="JNZ73_000423"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAY8730830.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36950..37708
                     /gene="ispU"
                     /locus_tag="JNZ73_000424"
                     /gene_synonym="uppS"
     CDS             36950..37708
                     /gene="ispU"
                     /locus_tag="JNZ73_000424"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HAY8730831.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37721..38578
                     /gene="cdsA"
                     /locus_tag="JNZ73_000425"
     CDS             37721..38578
                     /gene="cdsA"
                     /locus_tag="JNZ73_000425"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAY8730832.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWCADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38590..39942
                     /gene="rseP"
                     /locus_tag="JNZ73_000426"
     CDS             38590..39942
                     /gene="rseP"
                     /locus_tag="JNZ73_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HAY8730833.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRVAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39972..42404
                     /gene="bamA"
                     /locus_tag="JNZ73_000427"
     CDS             39972..42404
                     /gene="bamA"
                     /locus_tag="JNZ73_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HAY8730834.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFREGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42526..43011
                     /gene="skp"
                     /locus_tag="JNZ73_000428"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42526..43011
                     /gene="skp"
                     /locus_tag="JNZ73_000428"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HAY8730835.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43015..44040
                     /gene="lpxD"
                     /locus_tag="JNZ73_000429"
     CDS             43015..44040
                     /gene="lpxD"
                     /locus_tag="JNZ73_000429"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAY8730836.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44145..44600
                     /gene="fabZ"
                     /locus_tag="JNZ73_000430"
     CDS             44145..44600
                     /gene="fabZ"
                     /locus_tag="JNZ73_000430"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAY8730837.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44604..45392
                     /gene="lpxA"
                     /locus_tag="JNZ73_000431"
     CDS             44604..45392
                     /gene="lpxA"
                     /locus_tag="JNZ73_000431"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAY8730838.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45392..46540
                     /gene="lpxB"
                     /locus_tag="JNZ73_000432"
     CDS             45392..46540
                     /gene="lpxB"
                     /locus_tag="JNZ73_000432"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HAY8730839.1"
                     /translation="MTDQRPLTIALVAGEISGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46537..47133
                     /gene="rnhB"
                     /locus_tag="JNZ73_000433"
     CDS             46537..47133
                     /gene="rnhB"
                     /locus_tag="JNZ73_000433"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAY8730840.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47170..50652
                     /gene="dnaE"
                     /locus_tag="JNZ73_000434"
     CDS             47170..50652
                     /gene="dnaE"
                     /locus_tag="JNZ73_000434"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAY8730841.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50665..51624
                     /gene="accA"
                     /locus_tag="JNZ73_000435"
     CDS             50665..51624
                     /gene="accA"
                     /locus_tag="JNZ73_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAY8730842.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51723..53864
                     /gene="ldcC"
                     /locus_tag="JNZ73_000436"
     CDS             51723..53864
                     /gene="ldcC"
                     /locus_tag="JNZ73_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HAY8730843.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53921..54310
                     /locus_tag="JNZ73_000437"
     CDS             53921..54310
                     /locus_tag="JNZ73_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY8730844.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54375..55673
                     /gene="tilS"
                     /locus_tag="JNZ73_000438"
     CDS             54375..55673
                     /gene="tilS"
                     /locus_tag="JNZ73_000438"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAY8730845.1"
                     /translation="MTLTLNRPLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55722..55982)
                     /gene="rof"
                     /locus_tag="JNZ73_000439"
     CDS             complement(55722..55982)
                     /gene="rof"
                     /locus_tag="JNZ73_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HAY8730846.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55969..56169)
                     /locus_tag="JNZ73_000440"
     CDS             complement(55969..56169)
                     /locus_tag="JNZ73_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HAY8730847.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56335..56868
                     /locus_tag="JNZ73_000441"
     CDS             56335..56868
                     /locus_tag="JNZ73_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HAY8730848.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAW"
     gene            56877..57299
                     /gene="arfB"
                     /locus_tag="JNZ73_000442"
     CDS             56877..57299
                     /gene="arfB"
                     /locus_tag="JNZ73_000442"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY8730849.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     APSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57313..58023
                     /gene="nlpE"
                     /locus_tag="JNZ73_000443"
                     /gene_synonym="cutF"
     CDS             57313..58023
                     /gene="nlpE"
                     /locus_tag="JNZ73_000443"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HAY8730850.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58178..59002)
                     /locus_tag="JNZ73_000444"
     CDS             complement(58178..59002)
                     /locus_tag="JNZ73_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HAY8730851.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRNIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59055..60773)
                     /gene="proS"
                     /locus_tag="JNZ73_000445"
     CDS             complement(59055..60773)
                     /gene="proS"
                     /locus_tag="JNZ73_000445"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAY8730852.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60884..61591)
                     /gene="tsaA"
                     /locus_tag="JNZ73_000446"
     CDS             complement(60884..61591)
                     /gene="tsaA"
                     /locus_tag="JNZ73_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAY8730853.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61588..61992)
                     /gene="rcsF"
                     /locus_tag="JNZ73_000447"
     CDS             complement(61588..61992)
                     /gene="rcsF"
                     /locus_tag="JNZ73_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HAY8730854.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62110..62925)
                     /gene="metQ"
                     /locus_tag="JNZ73_000448"
     CDS             complement(62110..62925)
                     /gene="metQ"
                     /locus_tag="JNZ73_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HAY8730855.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62965..63618)
                     /gene="metI"
                     /locus_tag="JNZ73_000449"
     CDS             complement(62965..63618)
                     /gene="metI"
                     /locus_tag="JNZ73_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HAY8730856.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63611..64642)
                     /gene="metN"
                     /locus_tag="JNZ73_000450"
     CDS             complement(63611..64642)
                     /gene="metN"
                     /locus_tag="JNZ73_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HAY8730857.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64830..65405
                     /gene="gmhB"
                     /locus_tag="JNZ73_000451"
     CDS             64830..65405
                     /gene="gmhB"
                     /locus_tag="JNZ73_000451"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAY8730858.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15511 a        16251 c        18081 g        15770 t
ORIGIN      
        1 atgggattga tatttatctg cgggaattta tattatgttt ctgcaaaaat gtttaaacta
       61 aaatttttat taaggaaaga gaggatgtat gattttcaga aaatatttat attcgtaata
      121 taaataaatt accatttgct taatttgtca aatgctcttg tatgtaagac ttcacgcaac
      181 gtctccccat caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat
      241 ccacgattct tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta
      301 ccaaacagca tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca
      361 cgaccctgtt gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgggcgaa
      421 gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtggg
      481 gtgcggtaaa acgaagaaac ggcaagaatg cggctttcag ggatatcgcc taatgctttc
      541 agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc
      601 actgtcatgt ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacgacgac
      661 gcggtgacgg ttcttcatcc agctcgttaa gcatcccttt ttgatctggt ggtgcggaaa
      721 cctggaactc accccaccat ttcaccagac gctgcagttc agcattacgt tcaacttcag
      781 ctcgcaaggc caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg
      841 cgcgtttacc ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg
      901 tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg
      961 ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct
     1021 ccagcagtgg gtaccagaac atggcggcaa acaagaacgc cgggttcacg cgcatatcgt
     1081 tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt
     1141 cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt
     1201 cacacaacag cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt
     1261 caaacaggcg tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg
     1321 cggtttccgg gctgatgcgc atacccaatt tggcggcaaa acgtaccgcg cgcagcatac
     1381 gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct
     1441 tcagatcctt cataccgcca acgtaatcac ggacggtaaa atccgctacg ctgtaataca
     1501 ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt
     1561 cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt
     1621 cgtggtgtcc acggaaggtc gcaacttcga taatctccgg gccaaacatt acatgagcca
     1681 gacggaaacg gcgacccacc aggcggcagt tacggaacag tttgcgcacc tgctcaggcg
     1741 tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc
     1801 cgccgccaac cagccaggct tcgtatcccg ctttgttgag cctgtacatt accttcaggg
     1861 cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct
     1921 gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa
     1981 aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa
     2041 aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc
     2101 cgactcgggc acggcggtca gcatccagtt tttgacggct gactgaagga tttgctcgac
     2161 gctgaaatcc tgccagtgtg cttctgcctg ctgccccaga aattgaagtg ccgcgattag
     2221 taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc
     2281 gccttgtgga ttaagcgcca gcggcagatg aatgtaatct ggcactttcc agccaaaaag
     2341 ctggtacagc gagatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc
     2401 tgtaacgccc tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc
     2461 acggcgatga atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgcctcg
     2521 cagcaggtca gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc
     2581 tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg
     2641 cgcacgcgta caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc
     2701 acgataggcg tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta
     2761 atgttccagc tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat
     2821 atcttctatg cgtaccagcc agcgaccttg ccgggcgcga ccctgcaaat agctgccgag
     2881 cgcggcgatc agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat
     2941 atactgtgtg tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg
     3001 gtaaacatga tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag
     3061 cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat
     3121 ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt
     3181 ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc
     3241 ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg
     3301 tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg
     3361 gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc
     3421 caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat
     3481 gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta
     3541 taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg
     3601 aggaaaagcg tattcgcgca cgcgaccaga atgtaaatta accagttact tactttacta
     3661 caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta
     3721 agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc
     3781 gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaagataac
     3841 cgcacgctgg ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc
     3901 agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc
     3961 aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttcgga
     4021 gccgtatttt acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt
     4081 aaggatagcc tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa taaatgcgcc
     4141 gctctggctt tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt
     4201 cgaataccag acggtatcgc caggcgttgc acaaccggtc atcgcacccg tattcgggca
     4261 gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg
     4321 ctgaattagc gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa tccttgttat
     4381 tgcgttagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag
     4441 gcgtaaaggg tgaactccgt caccgcataa gaccagttaa aacccggcgg cgggaatgtc
     4501 accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga
     4561 aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt
     4621 ggaggctgac gcatccctaa ccacaccaca cgcgaacgca gccattgtcc ggcgtcatca
     4681 agcgtgagtg tgaaacctgg ttgacgaatc cgtccggcta aaagagaaag cgccttctct
     4741 ttctctgcgc tcacttcgcc taaaaatgcc agagtcagat gcaaattatc ggcggcgacc
     4801 ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccaat ggataatctg ttcgcgggtt
     4861 tctgcaggta agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg
     4921 ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt
     4981 gtgtcgtcgt tgcccgttgc tgccgtctta cctgaattac ttaccgctct cgattgtgcg
     5041 ccgcaggtat tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa
     5101 ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg
     5161 gcgcgtaacg tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg cgataccgtt
     5221 ggctaccgga tgcgtgcgca aaactgcgtc gggccgaata cccgcctgga agtggttacc
     5281 gaaggcgtgc tgacgcggat gatccagcgt gacccggaac tgagcggtgt tggattggtg
     5341 atccttgatg agtttcatga gcgcagtttg caggcggatt tggcgttggc gctgttactc
     5401 gatgtgcaac aaggtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac
     5461 aatgaccgct tgcagcaaat gctgccggaa gcgcccgtcg tcatctcaga agggcgctcg
     5521 tttccggttg aacgccgtta tttaccgctg cccgcgcatc agcgttttga cgaagccgtt
     5581 gcggtagcca ccgccgaaat ggtgcgtcag gaaagtggat cattactgtt atttttacct
     5641 ggcgtcggag aaattcagcg cgtgcaggaa caactggctt cgcgcatcgg cagtgatgtg
     5701 gtgctctgcc cgctgtatgg cgcgttgtca ctgaacgatc agcgaaaagc gatcctcccg
     5761 gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc
     5821 atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ctttgatccg
     5881 cgcacggggc ttacgcggtt gattactcaa cgcgttagtc aggcatccat gacgcagcgt
     5941 gccgggcgcg ccgggcgtct ggagccgggt atcagcctgc atttaatcgc caaagaacaa
     6001 gcagaacgcg ccgcggcgca aagtgagccg gagatcttac aaagcgatct ttccggtttg
     6061 ctgatggaat tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa
     6121 ccgccaacgg tgaatctact ggccgcgaaa cgcctgttac aaatgctggg ggcgctggag
     6181 ggtgaacggc ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga tccgcgttta
     6241 gcggcaatgc tggttaacgc aaagagcgat gacgaagctg ctaccgcggc aaaaattgcc
     6301 gccattctcg aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc
     6361 aatcaaccag cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgcggc
     6421 ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt
     6481 gctcatcgcc gtgggcaaga tggacgctat caactggcaa acggcatggg agcgatgctc
     6541 gatgccgacg acgcgctaag ccgccacgaa tggttgatcg caccgttatt attgcagggc
     6601 agcgcctcgc cggatgcgcg gattttactg gcgctaccgg tcgatattga tgaattagta
     6661 caacgctgtc cgcagctggt gcagcaatct gacaccgtgg aatgggatga cgcgcaaggt
     6721 acgctgaaag cctggcgtcg gctacaaatc ggtcagttga cggtgaaagt gcagccgctg
     6781 gcgaaaccgt cagaagacga gttgcatcag gcgatgctta atggcatccg tgataaaggt
     6841 ttaagcgtgc tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc
     6901 gcaaagtggt tgccggaata tgactggcca gcggttgatg atgaaagttt gttggcgacg
     6961 ctggaaacgt ggctgctgcc acatatgacc ggcgtacatt cactacgcgg cctgaaatca
     7021 ctcgacattt atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat
     7081 agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat
     7141 gaagataacc cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga ggccactaat
     7201 ccgacgatcg cccaggggcg cgtgccgctg gtgctgaagt tgctttcccc tgcccaaagg
     7261 ccgctgcaaa tcacgcgtga tttgggggcc ttctggaaag gggcgtatcg tgaggtgcaa
     7321 aaggagatga aagggcgtta tcccaaacac gtctggccga acgacccggc aaacacggca
     7381 ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga
     7441 gggccggggt gagggcatca gcgcgcacgt tcaccctcac cctaacccta accctctccc
     7501 tcaagggaga ggggaccgat cgagcacaaa ttttgagaga tatcttcttc tgtcttgtaa
     7561 cagaagaaca gaaaatcggg cttttgcgcc tgaatattgc ggagaaaaag catggccggg
     7621 aatgaccgcg agccaattgg acgcaaaggg aaaccgacgc gtccggtcaa acaaaaggta
     7681 agccgtcgtc gttacgaaga tgacgatgat tacgacgatt atgatgacta tgaggatgaa
     7741 gaaccgatgc cgcgcaaagg taagggcaaa ggcaaagggc gtaagcctcg tggcaaacgc
     7801 ggctggctat ggctactgct aaaactggct atcgtttttg ccgtgctgat cgccatttac
     7861 ggcgtttatc tcgatcaaaa aattcgtagc cgtattgatg gcaaggtctg gcaactgccc
     7921 gcggcagttt atggccgaat ggtcaacctt gaaccagata tgaccatcag caagaacgaa
     7981 atggtgaagc tgctggaggc aacacagtat cgtcaggtgt cgaaaatgac ccgtcctggc
     8041 gaatttaccg tgcaggccaa cagcattgag atgatccgcc gtccgtttga tttcccggac
     8101 agtaaagaag ggcaggtgcg tgcgcgtctg acctttgatg gtgatcatct ggcggccatc
     8161 gtcaatatgg agaacaaccg tcagttcggt ttcttccgtc ttgatccgcg tctgatcacc
     8221 atgatctcct cgccaaacgg tgagcagcgt ttgtttgtgc cgcgcagtgg tttccctgat
     8281 ttgctggtgg atactttgct ggcgacagaa gaccgtcatt tttacgagca tgatggaatc
     8341 agtctctact ccattgggcg tgcggtgctg gcaaacctga ccgcaggtcg cacggtgcag
     8401 ggtgcgagta cgctgacgca acagctggtg aaaaacctgt tcctctccag cgagcgttct
     8461 tactggcgta aagcgaacga agcttacatg gcgctgatca tggacgcgcg ttacagcaaa
     8521 gaccgtattc ttgagctgta tatgaacgag gtgtatctcg gtcagagcgg cgacaacgaa
     8581 atccgcggct tcccgctggc aagcttgtat tactttggtc gcccggtaga agagctaagc
     8641 ctcgaccagc aggcgctgtt agtcggtatg gtgaaagggg cgtccatcta caacccgtgg
     8701 cgtaacccga aactggcgct ggagcgacgt aatctggtgc tgcgtctgct gcaacagcaa
     8761 cagattattg atcaagaact ctatgacatg ttgagtgccc gtccgctggg ggttcagccg
     8821 cgtggtgggg tgatctctcc tcagccagcc tttatgcagt tggtgcgtca ggagttgcag
     8881 gcaaaactgg gcgataaggt aaaagatctc tccggcgtga agatcttcac tacctttgac
     8941 tcggtggccc aggacgcggc agaaaaagcc gccgtggaag gcattccggc actgaagaaa
     9001 cagcgtaagt tgagcgatct tgaaactgcg attgtggtcg tcgaccgctt tagtggtgaa
     9061 gttcgtgcga tggtcggagg ttctgagccg cagtttgcgg gctacaaccg tgcgatgcag
     9121 gcgcgtcgtt cgattggttc ccttgcaaaa ccagcgactt atctgacggc tttaagccag
     9181 ccgaaaatct atcgcctgaa cacgtggatt gccgatgcgc caatcgcgct gcgtcagccg
     9241 aatggccagg tctggtcacc gcagaacgat gaccgtcgtt atagcgaaag cggcagagtg
     9301 atgctggtgg atgcgttgac acgttcgatg aacgtgccga cggtaaatct ggggatggcg
     9361 ctggggctgc ctgcggttac ggagacctgg atcaaactgg gcgtaccgaa agatcagttg
     9421 catccggttc cggcaatgct gctgggggcg ttgaacttaa cgccaatcga agtggcgcag
     9481 gcattccaga ccatcgccag cggtggtaac cgtgcaccgc tttctgcact gcgttcggta
     9541 atcgcggaag atggtaaggt gctgtatcag agcttcccgc aggcagaacg cgctgttccg
     9601 gcgcaggcgg cgtatctgac actatggacc atgcagcagg tggtacaacg tggtacgggt
     9661 cgtcagcttg gggcgaaata cccgaacctg catctggcag ggaaaacagg gactaccaac
     9721 aataacgtag atacctggtt tgcaggcatt gacggcagca cggtgaccat cacctgggta
     9781 ggccgtgata acaaccagcc gaccaaactg tatggagcca gcggggcaat gtcgatttat
     9841 cagcgttatc tggctaacca gacgccaacg ccgctgaatc ttgttccgcc agaagatatt
     9901 gcagatatgg gcgtggacta cgacggcaac tttgtttgca gcggtggcat gcgtgtcttg
     9961 ccggtctgga ccagcgatcc gcaatcgctg tgccagcaga gcgagatgca gcagcagccg
    10021 tcaggcaatc cgtttgatca gtcttctcag ccgcagcaac agccgcaaca gcaacctgct
    10081 cagcaagagc agaaagacag cgacggtgta gccggttgga tcaaggatat gtttggtagt
    10141 aattaacatc taagcgtgaa ataccggatg gcgagttgcc atccggtaaa ataacatccc
    10201 atctaagata ttaacccttt cttttcatct ggttgtttat taacccttca ggaacgctca
    10261 gattgcgtac cgcttgcgaa cccgccagcg tttcgaatat tatcttatct ttataataat
    10321 cattctcgtt tacgttatca ttcactttac atcagagata taccaatggc gcgttccaaa
    10381 actgctcagc caaaacactc actgcgtaaa atcgcagttg tagtagccac agcggttagc
    10441 ggcatgtctg tttatgcaca ggcagcggtt gaaccgaaag aagacactat caccgttacc
    10501 gctgcacctg cgccgcaaga aagcgcatgg gggcctgctg caactattgc ggcgcggcag
    10561 tcagctaccg gcactaaaac cgatacgccg attcaaaaag tgccacagtc tatttctgtt
    10621 gtgaccgccg aagagatggc gctgcatcag ccgaagtcgg taaaagaagc gcttagctac
    10681 acgccgggtg tctctgttgg tacgcgtggc gcatccaaca cctatgacca cctgatcatt
    10741 cgcggttttg cggcagaagg ccaaagccaa aataactatc tgaatggcct gaagttgcag
    10801 ggcaacttct ataacgatgc ggtcattgac ccgtatatgc tggaacgcgc tgaaattatg
    10861 cgtggcccgg tttccgtgct ttacggtaaa agcagtcctg gcggtctgtt gaatatggtc
    10921 agcaagcgtc cgaccaccga accgctgaaa gaagttcagt ttaaagccgg tactgacagc
    10981 ctgttccaga ctggttttga ctttagcgat gcgttggatg atgacggtgt ttactcttat
    11041 cgcctgaccg gtcttgcgcg ttctgccaat gcccagcaga aagggtcaga agagcagcgt
    11101 tatgctattg caccggcgtt cacctggcgt ccggatgata aaaccaattt caccttcctt
    11161 tcttacttcc agaacgagcc ggaaaccggt tattacggct ggttgccgaa agagggaacc
    11221 gttgagccgc tgccgaacgg taagcgtctg ccgacagact ttaatgaagg ggcgaagaac
    11281 aacacctatt ctcgtaatga gaagatggtc ggctacagct tcgatcacga atttaacgac
    11341 acctttactg tgcgtcagaa cctgcgcttt ggtgaaaaca aaacctcgca aaacagcgtt
    11401 tatggttacg gcgtctgctc cgatccggcg aatgcttaca gcaaacagtg tgcggcatta
    11461 gcgccagcgg ataaaggcca ttatctggca cgtaaatacg tcgttgatga tgagaagctg
    11521 caaaacttct ccgttgatac ccagttgcag agcaagtttg ccactggcga tatcgaccac
    11581 accctgctga ccggtgtcga ctttatgcgt atgcgtaatg acatcaacgc ctggtttggt
    11641 tacgacgact ctgtgccact gctcaatctg tacaatccgg tgaatcccga tttcgacttc
    11701 aatgccaaag atccggcaaa ctccggccct taccgcattc tgaataaaca gaaacaaacg
    11761 ggcgtttatg ttcaggatca ggcgcagtgg gataaagtgc tggtcaccct aggcggtcgt
    11821 tatgactggg cagatcaaga atctcttaac cgcgttgctg ggacgaccga taaacgtgat
    11881 gacaaacagt ttacctggcg tggtggtgtt aactacctgt ttgataatgg tgtaacacct
    11941 tacttcagct atagcgaatc gtttgaacct tcttcgcaag ttgggaagga tggtaatatt
    12001 ttcgcaccgt ctaaaggtaa gcagtatgaa gtcggcgtga aatatgtacc ggaagatcgt
    12061 ccgattgtag ttactggtgc cgtgtataat ctcactaaaa ccaacaacct gatggcggac
    12121 cctgagggtt ccttcttctc ggttgaaggt ggcgagatcc gcgcacgtgg cgtagaaatc
    12181 gaagcgaaag cggcgctgtc ggcgagtgtt aacgtagtcg gttcttatac ttacaccgat
    12241 gcggaataca ccaccgatac tacctataaa ggcaatacgc ctgcacaggt gccaaaacac
    12301 atggcttcgc tgtgggctga ctataccttc tttgacggtc cgctttcagg tttgacgctg
    12361 ggcaccggtg gtcgttatac tggctccagc tatggtgatc cggctaactc ctttaaagtg
    12421 ggaagttata cggtcgtgga tgcgttagtg cgttatgatc tggcgcgagt cggcatggcg
    12481 ggctccaacg tggcgctgca tgttaacaac ctgttcgatc gtgaatacgt cgccagctgc
    12541 tttaacactt atggctgctt ctggggcgca gaacgtcagg tcgttgcaac cgcaaccttt
    12601 cgtttctaat ttctcttttg gggcacggat ttccgtgccc atttcacaag ttggctgtta
    12661 tgcaggaata cacgaatcat tccgatacca cttttgcact gcgtaatatc tcctttcgtg
    12721 tgcccgggcg cacgcttttg catccgctgt cgttaacctt tcctgccggg aaagtgaccg
    12781 gtctgattgg tcacaacggt tctggtaaat ccactctgct caaaatgctt ggtcgtcatc
    12841 agcctccgtc agaaggggag attcttcttg atgcccagcc gctggaaagc tggagcagca
    12901 aagcgtttgc ccgcaaagtg gcttatttgc cgcagcagct tcctccggca gaagggatga
    12961 ccgtgcgtga actggtggcg attggtcgtt acccgtggca tggcgcgctg gggcgctttg
    13021 gtgcggcaga tcgcgaaaag gtcgaggaag ctatctcgct ggttggctta aaaccgctgg
    13081 cgcatcggct ggtcgatagt ctctctggcg gcgaacgtca gcgggcgtgg atcgccatgc
    13141 tggtggcgca ggatagccgt tgtctgttgc tcgacgaacc gacttcggcg ctggatatcg
    13201 cccaccaggt tgatgtgctg gcgctggtgc accgtttaag tcaggaacgt ggcctgacgg
    13261 tcattgccgt gttgcacgat atcaatatgg cggcacgcta ctgtgattat ctggtcgccc
    13321 tgcgcagtgg tgaaatgatt gctcagggaa cgcctgcgga aattatgcgc ggcgaaaccc
    13381 tcgaaatgat ttatggcatc ccgatgggta ttttgccgca tccggcgggt gctgcacctg
    13441 tgagttttgt ttattgatga gcggcttacc tcttatttcg cgccgtcgac tgttaacggc
    13501 gatggcgctt tctccgttgt tatggcagat gaataccgcc cacgcggcgg ctattgaccc
    13561 caatcgtatt gtggcgctgg agtggttgcc ggtggaatta ctgctggcgc tcggcatcgt
    13621 gccttacggc gtggcggata ccatcaacta tcgcctgtgg gtcagcgaac caccattgcc
    13681 ggactcagtg attgacgtcg gtttgcgtac agaacctaac cttgaactgc tgaccgaaat
    13741 gaaaccgtcg tttatggtct ggtcggcagg atatggccct tcatcagaaa tgctggcacg
    13801 tattgcgccg ggtcgcggat ttaacttcag tgacggcaaa catccgttgg cgatggcgcg
    13861 taaatcgctg acggaaatgg cagatttact taacctgcaa agcgcagcgg aaacgcattt
    13921 agcgcaatac gaagacttta tccgcagcat gaaaccccgc tttgtgaagc gtggtgcacg
    13981 cccgttactg ctgacgacgc ttatcgatcc gcgccatatg ctggtcttcg gtccaaacag
    14041 cttgttccag gaaattctcg atgagtacgg catcccaaat gcctggcaag gggaaaccaa
    14101 cttctggggc agtaccgccg tcagtatcga tcgtctggca gcgtataaag acgttgatgt
    14161 gctctgtttt gatcacgaca acagcaaaga catggatgcg ctaatggcaa ccccgctgtg
    14221 gcaggccatg ccgtttgtcc gtacagggcg ctttcagcgc gtacccgcag tctggtttta
    14281 tggtgcaacg ctttcggcaa tgcactttgt gcgtgttctg gataacgcca tcggaggtaa
    14341 agcgtgagta aacgaattgc gcttttcccg gcgttattgc tggcgctgtt agtgattgtc
    14401 gctacggcgc tcacctggat gaacttctca caggcgctgc cgcgcagtca gtgggcgcag
    14461 gccgcctggt cgccggatat tgacgtcatc gagcagatga tttttcacta cagcttgttg
    14521 ccgcgtctgg cgatttcgct gctggtgggc gcgggcctgg ggctggtggg cgtgctgttt
    14581 cagcaagtac tgcgtaaccc gctggcggaa ccgacgacgt tgggcgttgc aacaggcgcg
    14641 caactgggga ttaccgtcac cacgctctgg gcgatcccta gcgcgatggc gagccagttt
    14701 gctgcgctgg caggggcttg cgttgttggc ttaattgtct ttggcgtcgc gtgggggaaa
    14761 cggctgtcgc cggtaacgct gattctcgcg gggttggtag tgagccttta ttgcggcgca
    14821 atcaatcagt tactggttat cttccatcat gaccagttgc aaagcatgtt tctgtggagc
    14881 actggaacgc tgacgcaaac cgactggggc ggtgttgagc gtttatggcc gcagttgctg
    14941 ggcggtgtga tgctgacgtt gctgctactt cgtccgttaa ccctgatggg gcttgatgat
    15001 ggcgtggcgc gcaatctcgg gctggccttg tcgcttgcgc gtctggcggc gctgtcgctg
    15061 gcgattgtca tcagtgcgct gctggtgaac gctgtgggga ttatcggctt tatcgggttg
    15121 ttcgcgccgc tgctggcaaa aatgctgggg gcgcggcgtc tgctgccacg actgatgctg
    15181 gcgtcgttga ttggtgcgct gatcctctgg ctttccgatc aaatcatcct ctggctgact
    15241 cgcgtgtgga tggaagtgtc caccggttcg gtcactgcgt tgatcggtgc gccgctgcta
    15301 ctgtggctgc tgccgcgttt acgcagcatt agcgcgccgg atatgaaggt taacgatcgt
    15361 gtcgcgactg aacgccaaca tgtgctggcg tttgccctcg cgggcggcgt gctgttgttg
    15421 atggctgtgg tggtggcgct gtcgtttggt cgtgatgcgc acggctggac gtgggcgagc
    15481 ggggcgttgc tcgaggattt aatgccctgg cgctggccgc gaattatggc ggcgctgttt
    15541 gcgggcgtca tgctggcggt agcgggctgt attattcagc gactgaccgg caacccgatg
    15601 gcaagcccgg aagtgctggg gattagctcc ggcgcggcgt ttggtgtggt gttaatgctg
    15661 tttctggtac cgggtaatgc ctttggctgg ttgttacccg caggcagtct cggggcggcg
    15721 gtgatgctgt tgatcattat gatcgccgcc ggacgcggtg gattttcccc acaccgcatg
    15781 ttactggcgg ggatggcgtt aagtaccgcg ttcaccatgc ttttgatgat gttgcaggca
    15841 agtggtgacc cgcgaatggc gcaagtgctg acctggattt ccggttcgac ctacaacgcg
    15901 accgatgctc aggtctggcg caccggaatt gtgatggtga ttttgctggc gattactccg
    15961 ctgtgccgcc gctggctgac cattttaccg ctgggtggtg ataccgcgcg ggcggtaggg
    16021 atggcgctga cgccgacgcg aattgcgctg ctgctgttag cggcttgcct gacggcgacc
    16081 gcgacgatga ctattggacc gttgagtttt gttggtttaa tggcaccgca tattgcgcgg
    16141 atgatgggct ttcgacggac gatgccacat atcgtaattt cggcgctggt gggtggttta
    16201 ctgctggtgt tcgctgactg gtgtgggcgg atggtgctgt ttccattcca gatcccggcg
    16261 gggctgctgt cgacctttat cggtgcgcca tattttatct atttgctgag aaagcagagc
    16321 cgttaataga atgcctcgcc cggtgggcga ggcaaaatta tcacaacttc gcgaacaccc
    16381 gacgtgcagc atcgatggtg ttattgatat cttccatgct gtgcgccaca gacataaagc
    16441 ccgcttcaaa cgctgacggt gccaggtaaa caccttcatc cagcatcata tggaagaaac
    16501 gcttaaaacg ctcgacgtca caggccatca catcctgata gcacgtcacg gactcggcgt
    16561 cggtaaagaa gataccgaac ataccaccga cgtggttaac gaccagcgga attcccgctt
    16621 cttctgccgc ttccagcaga ccttctgcca gacgtgttgt cagctcatcc agcgtttcgt
    16681 gaatgcccgg ctgcgcgact tcattcagac aggcgaaacc cgctgccatc gcaatcgggt
    16741 taccggaaag cgtacccgcc tgatagaccg gacctgtagg ggccagcgca tccattacat
    16801 cacgacgacc accgaatgcg cctaccggca ttccaccgcc gatgattttt ccgaggcagg
    16861 ttaaatctgg cactacgccg aaataatcct gtgcgccagc tagcgctacg cggaaaccgg
    16921 tcatcacttc atcgatgatc agcaacgcgc caaattcgtc gcacagcgca cgcagacctg
    16981 gcaggaactc tggcagcggc ggaacacagt tcatattgcc tgccaccggc tcgacgataa
    17041 tacaggcaat ctcttgcggg tattgctcaa acgcggcgcg tacagaggcc agatcgttat
    17101 aagtacaggt taaggtatgt ttggcgaaat ctgccggaac gcccggcgag tttggctggc
    17161 ctaacgtgag tgcgccagaa ccggctttca ccagcaggca gtcagcgtga ccgtggtaac
    17221 aaccttcaaa tttaatgatt ttgtcgcgac cggtaaaacc acgggccaag cggatggcgc
    17281 tcatcgtcgc ctcggtgccg gagttcacca tgcgcaccat atctatggtc gggaccagtt
    17341 cggtcatcag ttgcgccatt ttcacttcca tttcggtcgg tgcaccaaag cttaaaccac
    17401 gctcggcggc ttcaatcacg gcattgcgga tcgccggatg gttatggccc agcaccatcg
    17461 gcccccagga accgacataa tcgatatagg ctttgccatc aacatcgtac agataagcgc
    17521 cgtccgcttt ttcgataaac agtggagtgc cgcccacgcc agtaaaggcg cgaacagggg
    17581 agttcacacc gccagggatc agctcgcgcg ctgcgctgta aagattttca gacttactca
    17641 tggagggttc ctgattcgta gaaaaagtga atggctgcta ttctatgtta ttcataacaa
    17701 gttaaatact cgtcaaacat caggctgctt gtactggtgc aatcctgatt tcttagagta
    17761 taaaagtttt gtgcatttga aacattacgc tttgcaaagg attttcatgg aacgtgcgag
    17821 taaaatgccg tcatcttatt tgtatgacca ataagtgatc attggatgaa aactgatact
    17881 ccctctttag aaacaccgca ggccgcgcgc ctgcgacgca gacaactgat tcgccaactt
    17941 cttgagcgcg ataaaacccc gttagccatt ttgtttatgg cggcagtcgt cggcacgctt
    18001 atcgggctgg ctgcggttgc ttttgacaaa ggtgtcgcct ggttgcagaa ccaacgtatg
    18061 ggagtgctgg tacatactgc tgataattat ccgctactgt taaccgtcgc ttttctctgt
    18121 tcggcggtgc tggcgatgtt cggctacttt ttggtgcgca aatacgcgcc cgaagcaggg
    18181 ggatcgggga tcccggaaat tgaaggggcg ctggaagatc aacgtcctgt tcgctggtgg
    18241 cgtgtattgc cggtgaaatt ctttggcggg ctggggacac tcggcggagg catggtgcta
    18301 gggcgcgaag gaccaaccgt gcagatcggc ggcaatatcg gtcgcatggt gcttgatatt
    18361 ttccgcctga aaggtgatga agctcgccat acgctgctgg caaccggtgc tgctgcgggg
    18421 ctggctgcgg cctttaacgc gccgctggcg ggtattttgt ttattatcga agagatgcgt
    18481 ccgcagtttc gctatacgtt aatttcgatt aaagcggtat ttattggcgt cattatgtcg
    18541 accattatgt accggatttt taatcatgaa gttgcgttga ttgacgtcgg taaactttct
    18601 gatgcgccgc ttaatacgct gtggctttat ctgatcctcg gtattatttt tggcattttc
    18661 ggccctattt ttaataaatg ggtgctgggg atgcaggatt tgctgcaccg tgtgcacggc
    18721 ggcaatatta ccaaatgggt actgatgggg ggggcgattg gcggtctgtg tggattgctg
    18781 gggtttgtgg caccagcaac gtcgggcggc ggttttaacc tgattcctat cgctaccgcg
    18841 gggaatttca gcatgggaat gctggtgttt atcttcgtcg ctcgggtcat taccacctta
    18901 ctctgcttct cttccggcgc gccgggcggt atttttgccc cgatgctggc gctgggtact
    18961 gtgctgggaa ccgctttcgg aatggttgcc gttgagctgt ttccgcaata tcacctggag
    19021 gcggggacgt ttgctattgc cggaatgggg gcattactgg cggcatctat tcgcgcgccg
    19081 ttaacgggga tcattctggt tctggagatg accgataact accagctcat tttgccaatg
    19141 attattaccg gtcttggcgc aacactatta gcgcaattta ccggcgggaa acctctatac
    19201 tcggcgattc ttgcgcgcac gctggcaaaa caggaagctg agcaactggc gcgaagcaag
    19261 gccgcatcag ccagcgagaa tacttgaacg aaataccagg gtattagata atggcgatta
    19321 ttattgggtt agaatttgcc cgattgccga tgtcgtttgg agcaaaatat gagtgatgac
    19381 gtagcactgc cgctggagtt taccgacgca gcagccaaca aagttaaaag cctgatcgct
    19441 gacgaagata acccgaatct gaaattacgc gtgtatatca ccggtggcgg ttgcagcggc
    19501 ttccagtatg gtttcacctt tgatgatcag gtgaacgaag gcgatatgac catcgaaaaa
    19561 cagggcgttg gcctggtggt tgatccgatg agcctgcaat atctggtcgg cggttccgtt
    19621 gactacaccg aaggactgga aggttctcgt ttcatcgtga ccaacccgaa cgcgaaaagc
    19681 acctgcggtt gcggttcttc ctttagcatc taatctgttg ttcatgtgcc ggataaacta
    19741 tccggcacat cttctctctt caacgcccat tctcatccag cgcaaacgtc ggtagcttaa
    19801 gatgccaacg aatagccgcc agccgaatca atagcgtcac gaccatgccc atcatactgg
    19861 ctgtttccag tggtacggaa aatgtgtaat aagccgtagc gtggacaata ccgccgataa
    19921 tacaggcagt tgcgtagatt tctgtacgta aaatcatggg gatttcgcgg gccagtacat
    19981 cacgaatgat cccgccgcca acgccagtaa tgacgcccat acaaaccgcg attaacggac
    20041 cggcttccgc attaaaggct ttattcacgc caatgccgac aaacaccgcc agaccaacgg
    20101 cgtccagcac cggcaacatc cattttggta agcgtcttgg ctggcgcacc agcacgatgg
    20161 tcagcatgct ggtgaccatg gcaacgacca gatcggtggg atctttcacc caaaataccg
    20221 ggccgtgatc cagcgccatg tcgcgaattg ttccgtcgcc tactgcggta accacgccca
    20281 gtaccagaac accaaaaggg tccatacgca attttccggc taacaaaacg ccggagatgg
    20341 caaataccgc tgtgccgact atatccagcc aatagacgag cattgttcaa tccccactga
    20401 gcacctgaaa aggtcaggca ctaatctacc tgtgaaagcg cattacagag ctgttgtgct
    20461 gcgaggataa tacgtgggct tgcacgttca aaccagtcac tcgtgagagg aataacggga
    20521 attttgagct gttcacccca gtattgtttg attttaggaa tttggtccgg tccgcctgta
    20581 atgacaatcg cctgtggcga gcgtgctaac acctgttcgc ggctaacttg cggccaggga
    20641 acccggctgt ctttaaagat gttttctccg ccacaaactt cgagtacctg gttctgaatc
    20701 gactcttttc cactggtaaa tggcggatta atgccgaatt gcagaaaaac acgtttttta
    20761 ggtttatcag catattgcgc tttcaattgc acgtactgat ccagcaggga ttgcgcggct
    20821 tgttcggcct tgtctggttg cggactccag ggggccagtt gacgtaacgc attggcaatt
    20881 tgttcaatgc ttgtcgcatc gacccacatc acttttattc ccagcgaagc cagctggtca
    20941 acctgccgct cggcattacc tccacgccag gcaatcacca gatcgggttt cagcgcgaca
    21001 atgcgttcca gattcatccc ctgccaggtg gaaacctgct caatcttttg cgcttgtgga
    21061 ggatagtcgg aatagctgct gaccccaacc ggcgtgatcc cggcggcaaa ggcaagttca
    21121 gtgttggcgg gagaaagcgt gatgacgcgc ggcgcggcgt tgagccacag tggcgcgaga
    21181 aaagacaggg cgaccagcgc cctgaacagt gatttagcca tgtgccagtt tctgcaccag
    21241 tgactcaacc atcaggctgg actgtttagc ggcaacagcc aggaactcat cgaagctaag
    21301 atgagactgt tgatcggcca cgtcggagat ggcgcgtacg acaacaaacg ggacgttgaa
    21361 attgtggcag acatgggcga ttgccgtcgc ttccatctct acagcaatgg cctgtgggaa
    21421 gttgtggcgg attttcgcca gaccaacaga accgttgatg aaagcgtcgc cgctaacaat
    21481 caggccacgt acagcgttaa gattcagttc ggcaatgcag gcctcagcgg cagcgatcag
    21541 tttatcgtca gctttaaagc ctgtcggaca gcctggtaac tgaccgtatt cataaccaaa
    21601 tgccgtgaca tccgcgtcgt gataacgcgc ttcgtccgag acaacgatat cgcccacttt
    21661 cagcgttggt gccaggccac cggcagaacc ggtgttaata atcacatctg gcttgcagtg
    21721 ttccaacagc agagtggcac ccagcgccgc agcgacttta ccgatgcccg atttcagaag
    21781 cgcaacctcg gttccattca gttggccggt atagatttcg caaccgccga gactgatagt
    21841 ttgacggttt tcgattttgt cacgcagcag cgtaacttct tcttccattg caccaatgat
    21901 gccgattttc atagatttac tcgcgataag cccgatttga aggcatagtt taccatgcgc
    21961 ttacggggaa gcgtatttct cacgcgggag aggacatggc acagattgat ttccgaaaaa
    22021 aaataaactg gcatcgtcgt taccgttcac cgcagggcgt taaaaccgaa catgagatcc
    22081 tgcggatctt cgagagcgat cgcgggcgta tcatcaactc tccggcaatt cgtcgtctgc
    22141 aacaaaagac ccaggttttt ccactggagc gcaatgccgc cgtgcgcacg cgtcttaccc
    22201 actcgatgga agtccagcag gtgggacgct acatcgccaa agaaatttta agccgtctga
    22261 aagagcttaa attgctggaa gcatacggcc tggatgaact gaccggcccc tttgaaagca
    22321 ttgttgagat gtcatgcctg atgcacgata tcggcaatcc gccgtttggt cattttggcg
    22381 aagcagcgat aaatgactgg tttcgccaac gtttgcaccc ggaagatgcc gaaagccagc
    22441 ctctgactga cgatcgctgc agcgtggcgg cactacgttt acgggacggg gaagaaccgc
    22501 ttaacgagct gcggcgcaag attcgtcagg acttatgtca ttttgagggg aatgcacaag
    22561 gcattcgtct ggtgcataca ttgatgcgga tgaatctcac ttgggcacag gttggcggta
    22621 ttttaaaata tacccgtccg gcgtggtggc gtggcgaaac gcctgagaca catcactatt
    22681 taatgaaaaa gccgggttat tatctttctg aagaagccta tattgcccgg ttgcgtaaag
    22741 aacttaattt ggcgctttac agtcgttttc cattaacgtg gattatggaa gctgccgacg
    22801 acatctccta ttgtgtggca gaccttgaag atgcggtaga gaaaagaata tttaccgttg
    22861 agcagcttta tcatcatttg cacgaagcgt ggggccagca tgagaaaggt tcgctctttt
    22921 cgctggtggt tgaaaatgcc tgggaaaaat cacgctcaaa tagtttaagc cgcagtacgg
    22981 aagatcagtt ttttatgtat ttacgggtaa acaccctaaa caaactggta ccctacgcgg
    23041 cacaacgatt tattgataat ctgcctgcga ttttcgccgg aacgtttaat catgcattat
    23101 tggaagatgc cagcgaatgc agcgatcttc ttaagctata taaaaatgtc gctgtaaaac
    23161 atgtatttag ccatccggat gtcgagcagc ttgaattgca gggctatcgg gtcattagcg
    23221 gattattaga gatttatcgt cctttattaa gcctgtcgtt atcagacttt actgaactgg
    23281 tagaaaaaga acgggtgaaa cgtttcccta ttgagtcgcg cttattccac aaactctcga
    23341 cgcgccatcg gctggcctat gtcgaggctg tcagtaaatt accgtcagat tctcctgagt
    23401 ttccgctatg ggaatattat taccgttgcc gcctgctgca ggattatatc agcggtatga
    23461 ccgacctcta tgcgtgggat gaataccgac gtctgatggc cgtagaacaa taaccaggct
    23521 tttgtaaaga cgaacaataa atttttacct tttgcagaaa ctttagttcg gaacttcggg
    23581 ctataaaacg aatctgaaga acacagcaat tttgcgttat ctgttaatcg agactgaaat
    23641 acatgaaaaa aaccacatta gcactgagtg cactggctct gagtttaggt ttggcgttat
    23701 ctccgctctc tgcaacggcg gctgagactt cttcagcaac gacagcccag cagatgccaa
    23761 gccttgcacc gatgctcgaa aaggtgatgc cttcagtggt cagcattaac gtagaaggta
    23821 gcacaaccgt taatacgccg cgtatgccgc gtaatttcca gcagttcttc ggtgatgatt
    23881 ctccgttctg ccaggaaggt tctccgttcc agagctctcc gttctgccag gggggccagg
    23941 gcggtaatgg cggcggccag caacagaaat tcatggcgct gggttccggc gtcatcattg
    24001 atgccgataa aggctatgtc gtcaccaaca accacgttgt tgataacgcg acggtgatta
    24061 aagtccagct gagcgatggt cgtaagttcg acgcgaagat ggttggcaaa gatccgcgct
    24121 ctgatatcgc gctgatccaa atccagaacc cgaaaaaact gaccgcaatt aagatggcgg
    24181 attctgatgc actgcgcgtg ggtgattaca ccgtagcgat tggtaacccg tttggtctgg
    24241 gcgagacggt aacttccggg attgtctctg cgctggggcg tagcggcctg aatgctgaaa
    24301 actacgaaaa cttcatccag accgatgcag cgatcaaccg tggtaactcc ggtggtgcgc
    24361 tggttaacct gaacggcgaa ctgatcggta tcaacaccgc gatcctcgca ccggacggcg
    24421 gcaacatcgg tatcggtttt gctatcccga gtaacatggt gaaaaacctg acctcgcaga
    24481 tggtggaata cgggcaggtg aaacgcggtg agctgggtat tatggggact gagctgaatt
    24541 ccgaactggc gaaagcgatg aaagttgacg cccagcgcgg tgctttcgta agccaggttc
    24601 tgcctaattc ctcggctgca aaagcgggca ttaaagcggg tgatgtgatc acctcactga
    24661 acggtaagcc gatcagcagc tttgccgcac tgcgtgctca ggtgggtact atgccggtgg
    24721 gcagcaaact gaccctgggc ttactgcgcg acggtaagca ggttaacgtg aacctggaac
    24781 tgcagcagag cagccagaat caggttgatt ccagctccat cttcaacggc attgaaggcg
    24841 ctgagatgag caacaaaggt aaagatcagg gcgtggtagt gaacaacgtg aagacgggca
    24901 ctccggctgc gcagatcggc ctgaagaaag gtgatgtgat tattggtgcg aaccagcagg
    24961 cagtgaaaaa catcgctgaa ctgcgtaaag ttctcgacag caaaccgtct gtgctggcac
    25021 tgaacattca gcgcggcgac agcaccatct acctgttaat gcagtaatct ccctcaaccc
    25081 cttcctgaat acgggaaggg gttctcctta caatctgtga acttcgccac aactccatac
    25141 atcttcatca tcctttgggc atttgcacaa tgccgtacgt tacgtacttc cttatgctaa
    25201 gccgtgcata acggaggact tatggctggc tggcatcttg ataccaaaat ggcgcaggat
    25261 atcgtggcac gtaccatgcg catcatcgat accaatatca acgtaatgga tgcccgcggg
    25321 cgaattatcg gcagcggcga tcgtgagcgt attggtgaat tgcacgaagg tgcattgctg
    25381 gtactttcac agggacgagt cgtcgatatc gatgacgcgg tagcacgtca tctgcacggt
    25441 gtgcggcagg ggattaatct accgttacgg ctggaaggtg aaattgtcgg cgtaattggc
    25501 ctgacaggtg aaccagagaa tctgcgtaaa tatggcgaac tggtctgcat gacggctgaa
    25561 atgatgctgg aacagtcgcg gttgatgcac ttgttggcgc aggatagccg tttgcgggaa
    25621 gaactggtga tgaacctgat tcaggcagag gagaatactc ccgcacttct gaatgggcgc
    25681 aacggctggg gatcgatctc aatcaaccgc gagtggtggc tattgttgag gtcgacagcg
    25741 gtcagcttgg cgtggacagc gcaatggcgg agttacaaca actgcaaaac gcgctgacta
    25801 cgcccgagcg taataatctg gtggcgattg tctcgctaac cgaaatggtg gtgttgaaac
    25861 cggcgttgaa ctcttttggg cgctgggatg cagaagatca tcgtaagcga gttgaacaac
    25921 tgattacccg catgaaagag tacggccagc tgcgttttcg cgtttcactg ggcaactatt
    25981 ttaccggtcc tagcagtatt gcccgatcct atcgtacggc gaaaacgacg atggtggtgg
    26041 gtaaacagcg gatgccagaa agtcgctgct atttttatca ggatctgatg ttacctgtgt
    26101 tactcgacag tttgcgtggc gactggcagg ccaacgaact ggcgcgaccg ctggcgcggc
    26161 tgaaagcgat ggacaataac ggcttgctgc gacgaacgct ggcggcgtgg tttcgccaca
    26221 atgtgcaacc gctggcaacg tcaaaggcgt tgtttattca tcgtaatacc ctggagtatc
    26281 ggcttaatcg tatatcggaa ctgaccgggc ttgatttggg caattttgat gacaggttgc
    26341 tgctgtatgt ggcgttacaa ctggatgaag agcggtagat tatgcgttaa ggttgaggcg
    26401 gcgctggctc attcccctca gcccgaaggg gagaggggaa tgcccgagtt aaccgtcaat
    26461 tcgggattat ttacgcgtta gtttttccag atcggcttca atctcgctga tcttattggt
    26521 tacgactgac tccaggtgac gtaagtcgtc gaggatctta cgcttaagat caacttcgct
    26581 gcggtcacgc tgacagattt gatcaagctc atcaatgata taccgtagat tcgggctgat
    26641 ttcctggact tctttataac cctgacccac accatcagcg acgaccgttt tacgctgacg
    26701 cggatattta aacttaacgc tcttggcgaa aaactcgcct ttgtctttct ggaaatagat
    26761 tttcagaata tcgttgttgg cttcctgccg gaggctgtaa cgatcaattt cttcaggatt
    26821 ggtaataccc agacttttca gattgtcgta catagcgtta cctcaaaatg agtcagtaaa
    26881 ttgtgcttat tttagcattt ggcttggccc gcccggctcg actgtgatcg cagaaagcct
    26941 ttttcttcca gacttctatc atggcgtaaa aatcaaaaaa ttacctgctt tattgtggcg
    27001 atattccatg ggatggatta gcacatttat cttcgttttg cgaattacat gacagtccga
    27061 aacaagatga aaaagtattt aaaaaagggg gaggaagaat gagtaggacg aattattctg
    27121 agccagaaag catacgggca gcatcatgct gcccgtatat gtactcttaa tcgatggtac
    27181 gcagcagttc gttaatgccg actttgccgc gagttttcgc gtcaactttc ttaacgataa
    27241 ccgcacagta gaggctgtat ttgccatctt ttgacggcag attacctgaa acaaccacag
    27301 accccgccgg aacgcgaccg tagtggattt cgccggtttc acggtcgtaa atacgggtgc
    27361 tctgaccaat gtatacgccc atagagatga cggaaccttc ttcgacaatc accccttcaa
    27421 ccacttcaga gcgtgcgcca atgaagcagt tatcttcaat aatggtcggg ttagcctgca
    27481 gcggctccag cacgccgccg atgcccacgc caccggaaag gtggacgttt ttaccaatct
    27541 gcgcacaaga accgacggtc gcccaggtat caaccatggt gccttcatca acatatgcgc
    27601 cgatgttaac gtaagacggc atcagcacgg tgttacgggc aataaacgca ccctgacgta
    27661 ccgccgctgg tggcacaacg cggaagcctt ctttctggaa acgtgcttcg tcgtagtcgg
    27721 cgaatttcat cggcactttg tcgaagtagc ggctttctgc cccttcgatc acctgattat
    27781 cattaatacg gaaagagagc agcaccgctt ttttcaacca ctgatgcgtc acccactgac
    27841 cgtcaatttt ttccgctaca cgcagtgcgc cggaatccag cagggcgatc acctgattta
    27901 ccgcttcgcg ggtaacggtg tctgcattgg ctggcgtgat ctcggcacgg cgttcaaaag
    27961 cggtttcaat aatgttctgt aactgctgca ttgttaaact cttttcatat cagtaaacac
    28021 atcacccttt atcgtttgga ttgagggcct ctgtcaaccg ctgatgcact tcctgctgca
    28081 actcgttatt aagcgcacgc cggtcagcgg tggcaattat gaataaatct tctactcgct
    28141 cgccaatggt tgtaattcgg gcaccatgaa gcgaaattcc cagatcggca aaaattttcc
    28201 cgactcgcgc cagcagtcca ggttggtcga gggcgatcag ttcgaggaac gatttgcggt
    28261 cggtatgggt cggcaaaaac gttacttcgg tttcaacagt aaaatggcgt aatttggcgg
    28321 gttggcgacg gggctgcggt ggctgccagc tactttgcgt cagcacctgc tccagaccaa
    28381 accgaataac ctcatggcga tctgcggaca gcgggctgcc atcgggttcc agcacgataa
    28441 aggtatccat cgccataccg tcgcgagtgg tgaaaatttg tgcgtcgtga acacttaaat
    28501 tgcggcggtc taattcggca cagacggcgg caaacagata ggggcggtcc gggctccaga
    28561 taaaaatctc ggtgcctcca cgcgtagcct gcgggctaag caataccagc ggtttgctta
    28621 aatcatgctg taataaatgg cgggcatgcc aggccagttg atttgggcta tggcggacaa
    28681 aatagttagc acgacagcgt gaccaaattt ggtgcagcgc ctcttcgtcg atgttatcca
    28741 tgcgcagtag tgccagtgcc tggagttggt gatggcgaac ccgttcgcgc atatccggcg
    28801 tgttttgcat tccgcgtcgt agctgctttt cggtggcaaa atagagttcg cgcaacagac
    28861 tttgcttcca gctattccac agcgtttcgt tggtggcgca aatgtcagcc actgtcaggc
    28921 ataccagata gcgcagacga ttttccgttt gaacttcttc ggcaaactgc ttgatgactt
    28981 cagggtcctg aatatcgcgg cgttgtgctg tcaccgacat cagcaagtgc tggcgaacca
    29041 gccaggcaac cagctgtgtt tcgcgtgagt tcagcccgtg gagttctgca aaatgcacta
    29101 catcctgagc accgagaatg gagtggtcgc cgccgcgtcc tttggcgata tcgtgaaaca
    29161 gcgcggcgat gaaaatcagc tcagttgacg gcaggcgcgg ccagacgtcc acacacaacg
    29221 gatggcgctg gcgcgtttct tcactggcaa aactctccag tttcagcatc acgcggatag
    29281 tatgttcatc caccgtgtag gcgtggaaca gatcaaactg catctgcccg acgatatgcg
    29341 accattgcgg catatacgcg ccgagcacgc tatggcgatg cattggcaat agcccgcgcc
    29401 gcaccgctcc ggggtgacgc agaatgctca aaaacagttt tcgtgcttgc ggaatattac
    29461 acagcggttg ttgcagatgg cgacgggcat ggcgtaactg gcgcagcgtg gtggagtaaa
    29521 taccggtgat cgcactgttg cgcaccatgg tgtagaacat acgcaagatg gcttccggct
    29581 ggcgcataaa tagtgtttca tcacgcaggt cgattagcgt accgcgtagc tgaaactcat
    29641 cgtcgagtgg acgtggtttt tcgtcggcgg gaagggcgag gatggcttca tcgaacagtt
    29701 gcagcagcat ctggttgagt tcactgacgc ggcgtgtaac gcggaagtaa tccttcatca
    29761 tccgctcgac cggttcgttg ccttcaccac tgtaattcag acgctgagcg acgctaagtt
    29821 ggcgatcgaa taacagacga ttatcgtaac ggctgacgac cagatgcagg gcaaagcgaa
    29881 tacgccacag tatatgcaga cattcgttta attccgcccg ctccgctgag gttaagaagc
    29941 caaacccgac catttcatcc agcgatgttg cgccaaaatg acggcgggcc acccattgca
    30001 gagtgtgaat atcgcgcaag ccgccagggc tacttttgat gtctggttca aggttgtagc
    30061 tggtgccatg gtaacgctga tggcgctggt tctgttcttc aactttcgct gcgtagaact
    30121 tgtcggaagg ccagaatcct tcgctaaaaa tatgtttttg cagttcgagg aagagcgcaa
    30181 catcgccaat taataagcgg gattcgatta aattggtggc gacggttaaa tccgataacc
    30241 cttccaacat gcactcttca agcgtgcgca cactatgacc gacttccagc tttacatccc
    30301 agagcagcgt taacagctcg cccacttttt gcgcctgatc gtccgggagc tttttacggc
    30361 ttaaaatcag taaatcgatg tcagaaagtg gatgcagctc gccacgaccg tagccaccga
    30421 cggcaaccaa tgccaggtcg gcaatctggc tgaatcccgc ttcaatccat aatcgttgca
    30481 ggagctggtc tatgaactcg gtacgtgcct caatcaactg ttctgcagag atcccattgt
    30541 caaaggcatc acccagccaa cgctggaaag tatcgatatg ggcttttatc ccacagacgg
    30601 ttaattcatc acggggccag gcgcatggat tttgcggttg accgggcagg gtggagagag
    30661 cggtgtttgc gtactgttct ggaagggtat tcattgtgcg ccacccataa gattaaatta
    30721 tcgcattaaa aaagccggca ttcgccggct tcatcttatt cgtcgtgcga gattatcgcc
    30781 gggatggtgt catccttgcg tagcgtcaaa atttcgcagc cgttatcagt caccacaata
    30841 gtatgctcat attgtgcaga caagctgcga tctttggttt ttaccgtcca gccatctttc
    30901 atggtgcgga tctctttttt acccgcgttg accattggct caatggtgaa cgtcatcccc
    30961 ggtttcagca cgacgttggt ttcacgggag tcatagtgca gcacctgcgg ttcttcatgg
    31021 aagccgcgac caataccgtg tccgcaatat tcacgaacga cggagaagcc ttctgcttcg
    31081 acaaatttct gaatcgccgc accgatttcg cgcagattaa tgcctggttt taccatgcgt
    31141 agcgccaggt acaggctttc ttgcgtgatg cggcacaggc gttcgcccat gatggtcggc
    31201 ttaccgacga taaacatttt cgaggtatcg ccgtggaaac catctttgat tacggtgaca
    31261 tcaatgttaa cgatatcgcc atctttcagc agcttagcat cgtccgggat accgtggcac
    31321 accacttcat taatagagat gcaaacggat ttcggatagc cgtgatagcc gaggcaggca
    31381 gaaaccgcgt gttgttcatt aacaatgtaa tcattacaga tgcgatccag ctcgccggtg
    31441 ctgacgcccg gtttaacata cggttcgatc atctccagca cttcggcagc cagtcggcca
    31501 gcgacgcgca ttttttcgat atcttctggg gtcttgattg agatagccat taattctgtc
    31561 catcagcgtc ggtgataccg acaatatata tgtaagtgcc gtcaatggta tcacacccgg
    31621 gcaaattgag aatcattctg aatttcgcca aacgtgccac tgaaggtttt ctataataga
    31681 aaattcgacg tctgatgctg tacacagcgc caacaattat tggtgtccac gacgtatttg
    31741 tggtataaag cgcgccggac ttccgatcca tttcgtatac acagactgga cggaagcgac
    31801 aatctcactt tgtgtaacaa cacacacgta tcggcacata ttccggggtg ccctttgggg
    31861 tcggtaatat gggatacgtg gaggcataac cccaactttt atatagaggt tttaatcatg
    31921 gcaactgttt ccatgcgcga catgctcaag gctggtgttc acttcggtca ccagacccgt
    31981 tactggaacc cgaaaatgaa gccgttcatc ttcggtgcgc gtaacaaagt tcacatcatc
    32041 aaccttgaga aaactgtacc gatgttcaac gaagctctgg ctgaactgaa caagattgtt
    32101 tctcgcaaag gtaaaatcct tttcgttggt actaaacgcg ctgcaagcga agcggtgaaa
    32161 gacgctgctc tgagctgcga ccagttcttc gtgaaccatc gctggctggg cggtatgctg
    32221 actaactgga aaaccgttcg tcagtccatc aaacgtctga aagacctgga aactcagtct
    32281 caggacggta ctttcgacaa gctgaccaag aaagaagcgc tgatgcgcac tcgtgagctg
    32341 gagaaactgg aaaacagcct gggcggtatc aaagacatgg gcggtctgcc ggacgctctg
    32401 tttgtaatcg atgctgacca cgaacacatt gctatcaaag aagcaaacaa cttgggtatt
    32461 ccggtatttg ctatcgttga taccaactct gatccggacg gtgttgactt cgttatcccg
    32521 ggtaacgacg acgcaatccg tgctgtgacc ctgtacctgg gcgctgttgc tgcaaccgta
    32581 cgtgaaggcc gttctcagga tctggcttcc caggcggaag aaagcttcgt agaagctgag
    32641 taataaggct tgataactcc cccaaaatag ttcgagttgc agaaaggcgg caagctcgag
    32701 aattcccggg agcttacatc agtaagtgac cgggatgagc gagcgaagac aacgcatctg
    32761 cggcgcgaaa tatgaagggg gagagccctt atagaccagg tagtacacgt ttggttaggg
    32821 ggcctgcata tggccccctt tttcactttt atatctgtgc ggtttaatgc cgggcagatc
    32881 acatctccga ggattttaga atggctgaaa ttaccgcatc cctggtaaaa gagctgcgtg
    32941 agcgtactgg cgcaggcatg atggattgca aaaaagcact gactgaagct aacggcgaca
    33001 tcgagctggc aatcgaaaac atgcgtaagt ccggtgctat taaagcagcg aaaaaagcag
    33061 gcaacgttgc tgctgacggc gtgatcaaaa ccaaaatcga cggcaactac ggcatcattc
    33121 tggaagttaa ctgccagact gacttcgttg caaaagacgc tggtttccag gcgttcgcag
    33181 acaaagttct ggacgcagct gttgctggca aaatcactga cgttgaagtt ctgaaagcac
    33241 agttcgaaga agaacgtgtt gcgctggtag cgaaaattgg tgaaaacatc aacattcgcc
    33301 gcgttgctgc gctggaaggc gacgttctgg gttcttatca gcacggtgcg cgtatcggcg
    33361 ttctggttgc tgctaaaggc gctgacgaag agctggttaa acacatcgct atgcacgttg
    33421 ctgcaagcaa gccagaattc atcaaaccag aagacgtatc cgctgaagtg gtagaaaaag
    33481 aataccaggt acagctggat atcgcgatgc agtctggtaa gccgaaagaa atcgcagaga
    33541 aaatggttga aggccgcatg aagaaattca ccggcgaagt ttctctgacc ggtcagccgt
    33601 tcgttatgga accaagcaaa actgttggtc agctgctgaa agagcataac gctgaagtga
    33661 ctggcttcat ccgtttcgaa gtgggtgaag gcatcgagaa agttgagact gactttgcag
    33721 cagaagttgc tgcgatgtcc aagcagtctt aattatcaaa aaggagccgc ctgagggcgg
    33781 cttctttttg tgcccatctt gtaaattcag ctaacccttg tggggctgcg ctgaaaagcg
    33841 acgtacaatg tcgctagtat taattcattt caatcgttga cagtctcagg aaagaaacat
    33901 ggctaccaat gcaaaacccg tctataaacg cattctgctt aagttgagtg gcgaagctct
    33961 gcagggcact gaaggcttcg gtattgatgc aagcatactg gatcgtatgg ctcaggaaat
    34021 caaagaactg gttgaactgg gtattcaggt tggtgtggtg attggtgggg gtaacctgtt
    34081 ccgtggcgct ggtctggcga aagcgggtat gaaccgcgtt gtgggcgacc acatggggat
    34141 gctggcgacc gtaatgaacg gcttggcaat gcgtgatgca ctgcaccgcg cctatgtgaa
    34201 cgctcgtctg atgtccgcta ttccattgaa tggcgtgtgc gacagctaca gctgggcaga
    34261 agctatcagc ctgttgcgca acaaccgtgt ggtgatcctc tccgccggta caggtaaccc
    34321 gttctttacc accgactcag cagcttgcct gcgtggtatc gaaattgaag ccgatgtggt
    34381 gctgaaagca accaaagttg acggcgtgtt taccgctgat ccggcgaaag atccaaccgc
    34441 aaccatgtac gagcaattga cttacagcga agtgctggaa aaagagctga aagtcatgga
    34501 cctggcggcc ttcacgctgg ctcgtgacca taaattaccg attcgtgttt tcaatatgaa
    34561 caaaccgggt gcgctgcgcc gtgtggtaat gggtgaaaaa gaagggactt taatcacgga
    34621 ataattcccg tgatggataa ataagggtaa gattccgcgt aagtatcgcg ggggcgtaag
    34681 tctggttata aggcgttatt gttgcaggca gtttggtcac ggccagcgcg cagcaaccgg
    34741 agcgtacaaa agtacgtgag gatggcgagc actgctcggg gccaaaatgg caaataaaat
    34801 agcctaataa tccagacgat tacccgtaat atgtttaatc agggctatac ttagcacact
    34861 tccactgtgt gtgactgtct ggtctgactg agacaagttt tcaaggattc gtaacgtgat
    34921 tagcgatatc agaaaagatg ctgaagtacg catggacaaa tgcgtagaag cgttcaaaac
    34981 ccaaatcagc aaaatacgca cgggtcgtgc ttctcccagc ctgctggatg gcattgtcgt
    35041 ggaatattac ggcacgccga cgccgctgcg tcagctggca agcgtaacgg tagaagattc
    35101 ccgtactctg aaaatcaacg tgtttgatcg ttcaatgtct ccggccgttg aaaaagcgat
    35161 tatggcctcc gatctcggcc tgaacccgaa ctctgcgggt agcgacatcc gtgttccgct
    35221 gccgccgctg acggaagaac gtcgtaaaga tctgaccaaa atcgttcgtg gtgaagcaga
    35281 acaagcgcgt gttgcagtac gtaatgtgcg tcgtgacgcg aacgacaaag tgaaagcact
    35341 gttgaaagat aaagagatca gcgaagacga cgatcgccgt tctcaggacg atgtacagaa
    35401 actgactgat gctgcaatca agaaaattga agcggcgctg gcagacaaag aagcagaact
    35461 gatgcagttc tgatttcttg aacgacaaaa acgccgctca gtagatcctt gcggatcggc
    35521 tggcggcgtt ttgcttttta ttctgtctca actctggatg tttcatgaag caactcacca
    35581 ttctgggctc gaccggctcg attggttgca gcacgctgga cgtggtgcgc cataatcccg
    35641 aacacttccg cgtagttgcg ctggtggcag gcaaaaatgt cactcgcatg gtagaacagt
    35701 gcctggaatt ctctccccgc tatgccgtaa tggacgatga agcgagtgcg aaacttctta
    35761 aaacgatgct acagcaacag gggagccgca ccgaagtctt aagtgggcaa caagccgctt
    35821 gcgatatggc agcgcttgag gaggttgatc aggtgatggc agccattgtt ggtgctgctg
    35881 ggctgttacc tacgcttgct gcgatccgcg cgggtaaaac cattttgctg gccaataaag
    35941 aatcactggt tacctgcgga cgtctgttta tggacgccgt aaagcagagc aaagcgcaat
    36001 tgttaccggt cgatagcgaa cataacgcta tttttcagag tttaccgcaa cctatccagc
    36061 ataatctggg atacgctgac cttgagcaaa atggcgtggt gtccatttta cttaccgggt
    36121 ctggtggccc tttccgtgag acgccattgc gcgatttggc aacaatgacg cccgatcaag
    36181 cttgccgtca tccgaactgg tcgatgggac gtaaaatttc tgtcgattcg gctaccatga
    36241 tgaacaaagg tctggaatac attgaagcgc gttggctgtt taacgccagc gccagccaga
    36301 tggaagtgct gattcacccg cagtcagtga ttcactcaat ggtgcgctat caggacggca
    36361 gtgttctggc gcagctgggg gaaccggata tgcgtacgcc aattgcccac accatggcat
    36421 ggccgaatcg cgtgaactct ggcgtgaagc cgctcgattt ttgcaaacta agtgcgttga
    36481 catttgccgc accggattat gagcgttatc catgcctgaa actggcgatg gaggcgttcg
    36541 aacaaggcca ggcagcgacg acagcattga atgccgcaaa cgaaatcacc gttgctgctt
    36601 ttcttgcgca acaaatccgc tttacggata tcgctgcgtt gaatttatct gtactggaaa
    36661 aaatggatat gcgcgaacca caatgtgtgg acgatgtgtt atctgttgat gcgaacgcga
    36721 gtgaagtcgc cagaaaagag gtgatgcgtc tcgcaagctg aggataatcc ggctacagag
    36781 agtcgcgcta tttgttagcg tagggcttca atgatatagt ctgcgccatc tgatcgtaag
    36841 tagttggctt tataaggtca ggtatgccgt ggttttacac ggcttttttt tgtataggct
    36901 tcagtattcc tgagtaccgt aaaccctgtc agggaataaa aaacgcgtga tgttgtctgc
    36961 tactcaacca cttagcgaaa aattgccagc gcatggctgc cgtcatgttg cgatcattat
    37021 ggacggcaat ggtcgctggg caaaaaagca agggaagatt cgtgcctttg ggcataaagc
    37081 cggggcaaaa tccgtccgcc gggctgtctc ttttgcggcc aacaacggta ttgaagcgtt
    37141 aacgctgtat gcctttagta gtgaaaactg gaaccgacca gcgcaggaag tcagtgcgtt
    37201 aatggaactg tttgtgtggg cgctcgatag cgaagtaaaa agtctgcacc gacataacgt
    37261 gcgtctgcgt attattggcg ataccagtcg ttttaactcg cgtttgcaag aacgtattcg
    37321 taaatctgaa gcgctaacag ccggaaatac cggtctgacg ctgaatattg cggcgaacta
    37381 cggtggacgt tgggatatag tccagggagt caggcaactg gctgaaaagg tgcagcaagg
    37441 aaacctgcaa ccagatcaga tagatgaaga gatgttaaac cagcatgtct gtatgcatga
    37501 actggcccct gtagatttag taattaggac tgggggagag catcgcatta gtaacttttt
    37561 gctttggcaa attgcctatg ccgaacttta ctttacagat gttctctggc ccgatttcga
    37621 tgaacaagac tttgaagggg cgttaaatgc ctttgctaat cgagagcgtc gtttcggcgg
    37681 caccgagccc ggtgatgaaa cagcctgatg ggggtcgctt ttgctgaagt atcgcctgat
    37741 atctgctttt gtgttaatac ccgtcgtcat cgcggcgttg tttctgttgc cgccggtggg
    37801 gttcgccatt gtaacgctgg tggtctgcat gctggcagcg tgggaatggg gacagcttag
    37861 cggttttacc actcgttcgc agcgagtatg gttggcggtg ttatgcgggt tattgttggc
    37921 gctgatgctt tttctgttgc cggaatatca ccgaaatatt catcaaccgc tggttgaaat
    37981 ctcactttgg gcttcgttgg gttggtggat tgtcgcgcta ttgctggtgc tgttttaccc
    38041 aggttccgca gcaatctggc gtaactctaa aacattgcgc cttatttttg gcgtgctaac
    38101 cattgttccc ttcttctggg gcatgctggc gttacgggcc tggcactatg acgagaatca
    38161 ttacagtggc gcaatatggc tgctctatgt catgatcctg gtatggtgcg ctgactccgg
    38221 cgcatatatg tttggcaaat tgtttggtaa acataagctg gcaccgaagg tttctccggg
    38281 taaaacctgg caaggcttta taggtggact cgctactgca gcggtaatct catggggtta
    38341 tggcatgtgg gcgaatctcg acgtcgctcc cgtcacctta ctcatttgct ctattgtcgc
    38401 agcgttagcc tcagtgctcg gcgatctgac cgagagtatg tttaagcgtg aagcaggaat
    38461 taaggacagc ggtcatttaa ttccaggaca cggtggtatt ttagatcgta ttgatagcct
    38521 gacggctgcg gtaccggtct ttgcttgctt gttgttactg gtattcagga cgctttaacg
    38581 gaaggtaata tgctgagttt tctctgggat ttggcttcgt tcatcgttgc actgggtgta
    38641 cttatcaccg tgcatgaatt tggtcatttc tgggttgccc ggcgttgtgg tgttcgcgtt
    38701 gagcgtttct caatagggtt tggtaaggcg ctctggcggc gaactgataa gctcggcacc
    38761 gaatatgtta tcgccctgat cccgttgggc ggttatgtca aaatgctgga tgagcgcgca
    38821 gaaccggtcg ttccggaact ccgccaccat gccttcaata ataaatctgt cggccaacga
    38881 gtggcgatta ttgccgcagg tccggttgca aacttcattt ttgctatctt tgcctactgg
    38941 ctggttttta ttattggtgt gcctggcgta cgtccggtgg ttggcgaaat agcagccaat
    39001 tcgatagctg cggaagcaca aattgcacca ggtacggaac taaaagccgt agatggtatc
    39061 gaaacgcctg attgggatgc cgtgcgtttg cagttggtcg ataaaattgg cgatgaaagc
    39121 accaccatta cagtagcgcc atttggcagc gaccaacggc gggatgtaaa gctcgattta
    39181 cgtcactggg cgtttgagcc tgataaagaa gatccggtat cttcgctggg gattcgtcct
    39241 cgtgggccgc aaattgaacc tgtactggaa aatgtgcagc caaactcggc ggcaagcaag
    39301 gcaggtttgc aagcaggcga caggatcgtt aaagtcgatg gtcagccctt aacgcagtgg
    39361 gtgacctttg tgatgcttgt ccgggataac ccgggtaaat ccttagcgtt agaaatcgaa
    39421 aggcagggga gtcccttgtc tttgacatta atcccggaga gtaaaccggg taatggtaaa
    39481 gcgattggtt ttgtcggtat tgagccgaaa gtcattcctt tgccagatga gtataaagtt
    39541 gtacgccagt atgggccgtt caacgccatc gtcgaagcca cggacaaaac gtggcagctg
    39601 atgaagctga cggtcagtat gctgggaaaa ttgatcaccg gtgatgtgaa actgaacaac
    39661 ctcagtgggc cgatttctat cgccaagggg gctgggatga cagcggaact cggggtagtt
    39721 tattacctgc cgtttcttgc gcttattagc gtgaacttag ggataattaa cctgtttccg
    39781 ttgcccgtac ttgacggggg gcatctgctg ttccttgcga tcgaaaagat caagggcgga
    39841 ccggtatccg agcgggttca agacttttgt tatcgcattg gctcgattct gctggtgctg
    39901 ttaatggggc ttgcactttt caatgatttc tctcggttat gagagttagt taggaagaac
    39961 gcataataac gatggcgatg aaaaagttgc tcatagcgtc gctgctgttt agcagcgcca
    40021 ccgtatacgg tgctgaaggg ttcgtagtga aagatattca tttcgaaggc cttcagcgtg
    40081 tcgccgttgg tgcggccctc ctcagtatgc cggtgcgtac aggcgacacg gttaatgatg
    40141 aagatatcag taataccatt cgcgctctgt ttgctaccgg caactttgag gatgttcgcg
    40201 tccttcgtga tggtgatacc cttctggttc aggtaaaaga acgtccgacc attgccagca
    40261 ttactttctc cggtaacaaa tcggtgaaag atgacatgct aaagcaaaac ctcgaggctt
    40321 ctggtgtgcg tgtgggcgaa tccctcgatc gcaccaccat tgccgatatc gagaaaggtc
    40381 tggaagactt ctactacagc gtcggtaaat atagcgccag cgttaaagct gtcgtgaccc
    40441 cgctgccgcg caaccgtgtt gacctaaaac tggtgttccg ggaaggtgtg tcagctgaaa
    40501 tccagcaaat taacattgtt ggtaaccatg ctttcaccac cgacgaactg atctctcatt
    40561 tccaactgcg tgacgaagtg ccgtggtgga atgtggtagg cgatcgtaaa taccagaaac
    40621 agaaactggc gggcgacctt gaaaccctgc gcagctacta tctggaccgc ggttatgccc
    40681 gtttcaacat cgactctacc caggtcagtc tgacgccaga taaaaaaggt atttacgtca
    40741 cggtgaacat caccgaaggc gatcagtaca agctttctgg cgttgaagtg agcggcaacc
    40801 ttgccgggca ctccgctgaa attgagcagc tgactaagat cgagccgggc gagctgtata
    40861 acggcactaa agtgaccaaa atggaagatg acattaaaaa gcttctcggt cgctatggtt
    40921 atgcctatcc gcgcgtacag tcgatgcctg aaattaacga tgccgacaaa accgttaaat
    40981 tacgcgtgaa cgttgatgcg ggtaaccgtt tctacgtgcg taagatccgt tttgaaggta
    41041 acgatacctc gaaagatgcc gtcctgcgtc gcgaaatgcg tcagatggaa ggtgcatggt
    41101 tggggagcga tctggtcgat cagggtaagg agcgtctgaa tcgtctgggc ttctttgaaa
    41161 ctgtcgatac tgatacccaa cgtgttccgg gtagcccgga ccaggttgat gtagtctaca
    41221 aggtgaaaga acgtaacact ggtagcttca acttcgggat tggttacggt actgaaagtg
    41281 gcgtgagctt ccaggctggc gtgcaacagg ataactggtt aggtacaggt tatgctgttg
    41341 gtatcaacgg gaccaaaaac gattaccaga cctatgctga actgtcggta accaacccgt
    41401 acttcaccgt agatggcgta agcctcggtg gtcgtctctt ctataatgac ttccaggcag
    41461 atgacgccga cctgtccgac tataccaaca agagttatgg tacagacgtg acgttgggct
    41521 tcccgattaa cgaatataac tcgctgcgtg caggtctggg ttatgtacat aactccctgt
    41581 ccaacatgca gcctcaggtt gcgatgtggc gttatctgta ctctatgggt gaacatccga
    41641 gcacttctga tcaggataac agcttcaaaa cggacgactt cacgttcaac tatggttgga
    41701 cctataacaa gcttgaccgt ggttacttcc cgacagatgg ttcacgtgtc aacctgaccg
    41761 gtaaagtgac cattcctgga tcggataacg aatactacaa agtgacgtta gacacggcga
    41821 cttatgtgcc gatcgatgac gatcacaaat gggttgttct gggtcgtacc cgctggggtt
    41881 atggtgatgg tttaggcggc aaagagatgc cgttctacga gaacttctat gccggtggtt
    41941 ccagcaccgt gcgtggcttc cagtccaata ccattggtcc gaaagcagtt tacttcccgc
    42001 atcaggccag taattatgat ccggactatg attacgaatg tgcgactcag gacggcgcga
    42061 aagacctgtg taaatcggat gatgctgtag gcggtaacgc catggcggtt gccagcctcg
    42121 agttcatcac cccgacgccg tttattagcg ataagtatgc taactcggtt cgtacttcat
    42181 tcttctggga tatgggtacc gtttgggata caaactggga ttccagccaa tattctggat
    42241 atccggacta tagtgatcca agcaatatcc gtatgtctgc gggtatcgca ttacaatgga
    42301 tgtccccatt ggggccgttg gtgttctcct acgcccagcc gttcaaaaag tacgatggag
    42361 acaaggcaga acagttccag tttaacatcg gtaaaacctg gtaagtgttc tccacaaagg
    42421 aatgtagtgg tagtgtagcg atgactttag gcgatcaata taagatcgcc gggccacgca
    42481 aagaactgca ccctccggtg caaatgggat ggtaaggagt ttattgtgaa aaagtggtta
    42541 ttagctgcag gtcttggttt agcactggca acttctgctc aggcggctga caaaattgca
    42601 atcgtcaata tgggcagcct gttccagcag gtagcgcaga aaaccggtgt ttctaacacg
    42661 ctggaaaatg agttcaaagg ccgtgccagc gaactgcagc gtatggaaac cgatctgcag
    42721 gctaaaatga aaaaactgca gtccatgaaa gcgggcagcg atcgcaccaa actggaaaaa
    42781 gacgtgatgg ctcagcgcca gacttttgct cagaaagcgc aggcttttga gcaggatcgc
    42841 gcacgtcgtt ccaacgaaga acgcggcaaa ctggttactc gtatccagac tgctgtgaaa
    42901 tccgttgcca acagccagga tatcgatctg gttgttgatg caaacgctgt tgcttacaac
    42961 agcagcgatg taaaagacat cactgccgac gtactgaaac aggttaaata agtaatgcct
    43021 tcaattcgac tggctgattt agcgcagcag ttggatgcag aactacacgg tgatggcgat
    43081 atcgtcatca ccggcgttgc gtccatgcaa tctgcacaaa caggtcacat tacgttcatg
    43141 gttaacccaa aataccgtga gcatttaggc ttgtgccaag cgtccgcggt tgtcatgacc
    43201 caggacgatc ttcctttcgc gaaaagtgcc gcgctggtag tgaagaatcc ctacctgact
    43261 tacgcgcgca tggcgcaaat tttagatacc acgccgcagc ccgcgcagaa cattgcaccc
    43321 agtgcggtga tcgacgcgac ggcgaagctg ggtaacaacg tatcgattgg cgctaacgcg
    43381 gtgattgagt ccggcgttga actgggcgat aacgtgatta tcggtgccgg ttgcttcgta
    43441 ggtaaaaaca gcaaaatcgg tgcaggttcg cgtctctggg cgaacgtaac catttaccat
    43501 gagatccaga tcggtcagaa ttgcctgatc cagtccggaa cagtggtagg cgcagacggc
    43561 tttggttatg ccaacgatcg tggtaactgg gtgaagatcc cacagattgg tcgcgtaatt
    43621 attggcgatc gagtggagat cggtgcctgc acaaccatcg atcgcggcgc gctggatgac
    43681 actgttattg gcaatggcgt gatcattgat aaccagtgcc agattgcaca taacgtcgtg
    43741 attggcgaca atacggcggt tgccggtggc gtcattatgg cgggcagcct gaaaattggt
    43801 cgttactgca tgatcggcgg agccagcgta atcaacgggc atatggaaat atgcgacaaa
    43861 gtgacggtta cgggcatggg tatggtgatg cgtcccatca ctgaaccagg cgtctattcc
    43921 tcaggcattc cgctgcaacc caacaaagtc tggcgcaaaa ccgctgcact ggtgatgaac
    43981 attgatgaca tgagcaagcg tctgaaatcg cttgagcgca aggttaatca acaagactaa
    44041 cgttccatct tttgttcgcc aaactttacg gcctgtctca ttcttacgat tgcggcaggc
    44101 cgtgttatta ttgtcgtttc ttatattttg acaggaagag tatcttgact actaacactc
    44161 atactctgca gattgaagag attttagaac ttctgccgca ccgtttcccg ttcttactgg
    44221 tggatcgcgt gctggatttt gaagaaggtc gttttctgcg cgcagtaaaa aatgtctctg
    44281 tcaatgagcc attcttccag ggccatttcc ctggaaaacc gattttcccg ggtgtgctga
    44341 ttctggaagc aatggcacag gcaacaggta ttctggcgtt taaaagcgta ggaaaactgg
    44401 aaccgggtga gctgtactac ttcgctggta ttgacgaagc gcgcttcaag cgcccggtcg
    44461 tgcctggcga tcaaatgatc atggaagtca ctttcgaaaa aacgcgccgc ggcctgaccc
    44521 gttttaaagg ggttgctctg gtcgatggta aagtagtttg cgaagcaacg atgatgtgtg
    44581 ctcgtagccg ggaggcctga tacgtgattg ataaatccgc ctttgtgcat ccaaccgcca
    44641 ttgtggaaga gggcgcgtca attggcgcga acgcacacat tggtcctttt tgtatcgttg
    44701 gaccccatgt cgaaattggt gagggtaccg tactgaaatc tcacgttgtc gtgaatggtc
    44761 atactaaaat tggccgcgat aatgagattt atcagtttgc ctccatcggc gaagttaacc
    44821 aggatttgaa atatgctggc gaaccgaccc gtgtggaaat cggcgatcgt aaccgcattc
    44881 gcgaaagcgt caccattcat cgtggcacag tccagggcgg tggattgacg aaggtgggca
    44941 gcgacaactt actgatgatc aacgcgcaca ttgcgcacga ttgtacggta ggtaaccgct
    45001 gtattctcgc caacaacgca acgctggcgg gtcacgtatc ggttgacgac ttcgcgatca
    45061 tcggcggcat gaccgcagtc catcagttct gcatcattgg tgcgcatgtg atggttggcg
    45121 gctgctccgg tgtggcgcag gacgtccctc cttatgtcat tgcgcagggt aaccacgcaa
    45181 cgccgttcgg tgtcaatatc gaagggctga agcgccgcgg attcagccgt gaggcgatta
    45241 ccgctatccg caatgcgtat aagctaattt atcgtagcgg taaaacgctc gatgaagtga
    45301 aaccggaaat tgctgaactg gcggaaacat atccggaagt gaaagccttt accgatttct
    45361 ttgcacgctc aacgcgcggt ctgattcgtt aatgactgac cagcgtccat taacgattgc
    45421 cctggtcgcc ggagaaatct ccggcgatat cctgggggcc ggtttaatcc gcgctctgaa
    45481 agaacgtgtg cccaacgccc gctttgttgg tgttgccggg ccacgaatgc aggctgaagg
    45541 ctgcgaagcc tggtacgaaa tggaagaact ggcggtgatg ggcattgttg aagtgctcgg
    45601 tcgtctgcgt cgcttactgc atattcgtgc cgatctgaca aagcgttttg gcgaactgaa
    45661 gccagatgtt tttgttggta ttgatgcgcc cgacttcaat attactcttg aaggtaacct
    45721 caaaaagcag ggtatcaaaa ccattcatta cgtcagtcca tccgtctggg cgtggcgaca
    45781 gaaacgcgtt ttcaaaatag gcagagccac cgatctggtg ctcgcatttc tgcctttcga
    45841 aaaagcgttt tatgacaaat acaatgtacc gtgccgcttt atcggtcata ccatggctga
    45901 tgccatgccg ttagatccag ataaaaatgg tgcccgtgat gtgctgggga tcccttacga
    45961 tgcccactgt ctggcattgt tgccgggcag ccgtggtgca gaagtcgaaa tgcttagtgc
    46021 cgatttcttg aaaactgccc agcttttgcg ccagacatat ccggatctcg aaatcgtggt
    46081 gccgctggtg aatgccaaac gccgcgagca gtttgaacgc atcaaagctg aagtcgcgcc
    46141 agacctgtcg gttcatttgc tggatggaat gggccgtgag gcgatggtcg ccagcgatgc
    46201 ggcactactg gcgtcgggga cggcagccct ggagtgtatg ctggcgaaat gcccgatggt
    46261 ggtgggatat cgcatgaagc cttttacctt ctggttggcg aagcggctgg tgaaaactga
    46321 ttatgtctcg ctgccaaatc tgctggcggg cagagagtta gtcaaagagt tattgcagga
    46381 agagtgtgag ccgcaaaaac tggctgcggc gctgttaccg ctgttggcga acgggaaaac
    46441 cagccacgcg atgcacgata ccttccgtga actgcatcag cagatccgct gcaatgccga
    46501 tgagcaggcg gcacaagccg ttctggagtt agcacaatga tcgaatttgt ttatccgcac
    46561 acgcagctgg ttgcgggtgt ggatgaagtc ggacgcgggc cgttagttgg cgcggtcgtc
    46621 accgctgcgg tgatccttga cccggcgcgc ccgattgccg ggctgaatga ttccaaaaag
    46681 ctgagcgaaa aacgccgtct ggcgctctat gaagagatca aagagaaagc gttgagctgg
    46741 agtctgggcc gcgcggaacc ccacgaaatc gacgagctga acattcttca tgcgaccatg
    46801 ctggcgatgc agcgtgccgt cgctgggctg catattgcgc cggaatatgt gttgattgat
    46861 ggtaaccgct gcccgaaatt accgatgcct gcgatggctg tggtgaaagg ggatagccgc
    46921 gtaccagaaa tcagtgccgc gtctatccta gcgaaagtga cgcgtgacgc cgaaatggcg
    46981 gcgctggata ttgttttccc gcaatatggt tttgcccaac ataaagggta tccaaccgct
    47041 tttcatctgg aaaaactggc tgaatatggc gcgactgaac atcatcgtcg cagctttggg
    47101 cctgtcaaac gcgcactggg acttgcgtcc tgattcttgt gtcgagatta agtaaaccgg
    47161 aatctgaaga tgtctgaacc acgtttcgta cacctgcggg tgcacagcga ctactcgatg
    47221 atcgatggct tggccaaaac cgcaccgctg gtaaaaaagg cggcggcgtt gggtatgcca
    47281 gcactggcga tcaccgattt caccaacctt tgcggtctgg tgaagttcta cggagcggga
    47341 catggcgcag ggattaagcc tatcgtcggg gcagatttta atgtccagtg cgacctgctg
    47401 ggtgatgagt taacccacct gacggtactg gcggcgaaca ataccggcta tcagaatctg
    47461 gcgttgctga tctcaaaagc gtatcagcgc gggtacggtg ccgccgggcc gatcatcgat
    47521 cgcgactggc ttatcgaatt aaacgaaggg ttgatccttc tttccggcgg gcgcatgggc
    47581 gacgtcggac gcagtctttt gcgtggtaac agcgcgctgg tagatgagtg tgtcgcgttt
    47641 tatgaagaac acttcccgga tcgctatttt ctagagctga tccgcaccgg caggccggat
    47701 gaagaaagct atctgcacgc ggcggtggaa ctggcggaag cgcgcggttt gcccgtcgtg
    47761 gcgactaacg atgtgcgctt tatcgacagc agcgactttg acgcacacga aatccgcgtc
    47821 gcgatccacg acggctttac cctcgacgat cctaaacgcc cgcgtaacta ttcgccgcag
    47881 caatatatgc gtagcgaaga ggagatgtgt gagctgtttg ccgacatccc agaagccctt
    47941 gccaacaccg ttgagatcgc caaacgctgt aacgtaactg tgcgtcttgg tgaatacttc
    48001 ctgccgcagt tcccgaccgg ggacatgagc accgaagatt atctggtcaa gcgtgcaaaa
    48061 gaaggcctgg aagagcgtct ggcattttta ttccctgacg aggaagaacg tcttaaacgc
    48121 cgcccggaat atgacgaacg tctggagact gaacttcagg ttatcaacca gatgggtttc
    48181 ccgggctact tcctcatcgt tatggaattt atccagtggt cgaaagataa cggcgtaccg
    48241 gtaggaccag gccgtggctc cggtgcgggt tcactggtgg cctacgcgct gaaaatcacc
    48301 gacctcgatc cgctggaatt tgacctgctg ttcgaacgtt tccttaaccc ggaacgtgtc
    48361 tccatgcctg acttcgacgt tgacttctgt atggagaaac gcgatcaggt tatcgaacac
    48421 gtggcggaca tgtacggtcg tgatgcggta tcgcagatta tcaccttcgg tacgatggcg
    48481 gcgaaagcgg tgatccgcga cgtaggccgc gtgctggggc atccgtacgg ctttgtcgac
    48541 cgtatctcga aactgatccc gcccgatccg gggatgacgc tggcgaaagc gtttgaagcc
    48601 gagccgcagc tgccggaaat ctacgaagcg gatgaagaag ttaaggcgct gatcgacatg
    48661 gcgcgcaaac tggaaggggt cacccgtaac gccggtaagc acgccggtgg ggtggttatc
    48721 gcgccgacca aaattaccga ttttgcgccg ctttattgcg atgaagaggg caaacatccg
    48781 gtcacccagt ttgataaaag cgacgttgaa tacgccggac tggtgaagtt cgacttcctt
    48841 ggtttgcgta cgctcaccat catcaactgg gcgctggaga tgatcaacaa gcggcgggcg
    48901 aagaatggcg agccaccgct ggatatcgcc gcgatcccgc tggatgacaa gaaaagcttc
    48961 gacatgctgc aacgctcgga aaccacggcg gtattccagc ttgaatcgcg cggcatgaag
    49021 gacctgatca agcgtctgca acctgactgc ttcgaagata tgatcgcact ggtggcactg
    49081 ttccgccctg gtccgttgca gtcagggatg gtggataact ttatcgaccg taaacatggt
    49141 cgtgaagaga tctcctatcc ggacgtgcag tggcagcatg aaagcctgaa atcggtactg
    49201 gagccaacct acggcattat cttgtatcag gaacaggtca tgcagattgc ccaggtgctt
    49261 tctggttata ccctcggtgg cgcggatatg ctgcgccgtg cgatgggtaa gaaaaagccg
    49321 gaagagatgg ctaagcagcg ttctgtattt gctgaaggtg cagaaaagaa cggaatcaac
    49381 gccgaactgg cgatgaaaat cttcgacctg gtggagaaat tcgcgggtta cggatttaac
    49441 aaatcgcact ctgcggccta tgctttggtg tcatatcaaa cgttatggct gaaagcgcac
    49501 tatccggcgg agtttatggc ggcggtaatg accgccgata tggacaacac cgagaaggtg
    49561 gtgggcctgg tggatgagtg ctggcggatg gggctgaaaa tcctgccacc agatataaac
    49621 tccggtcttt accatttcca cgtcaacgac gacggcgaaa tcgtgtatgg tattggcgcc
    49681 atcaaagggg taggtgaagg tccgattgag gccatcatcg aagcccgtaa taaaggcggc
    49741 tacttccgcg aactgtttga tctctgcgcc cgtaccgaca ccaaaaagtt aaaccggcgg
    49801 gtgctggaaa aactgatcat gtccggggcg tttgaccgtc ttgggccaca ccgcgcggcg
    49861 ctgatgaact cgctgggtga tgcgttaaaa gcggcagatc aacacgcgaa agcggaagct
    49921 atcggtcagg ccgatatgtt cggcgtgctg gccgaagagc cggaacaaat tgaacaatcc
    49981 tacgccagct gccaaccgtg gccggagcag gtggtattag atggggaacg tgaaacgtta
    50041 ggcctgtacc tgaccggaca ccctatcaac cagtatttaa aagagattga gcgttatgtc
    50101 ggaggcgtaa ggctgaaaga catgcacccg acagaacgtg gtaaagtcat cacagctgcg
    50161 gggctcgttg ttgccgcgcg ggttatggtc accaagcgcg gcaatcgtat cggtatctgc
    50221 acgctggatg accgttccgg gcggctggaa gtgatgttgt ttactgacgc cctggataaa
    50281 taccagcaat tgctggaaaa agaccgcata cttatcgtca gcggacaggt cagctttgat
    50341 gacttcagcg gtgggcttaa aatgaccgct cgcgaagtga tggatattga cgaagcccgg
    50401 gaaaaatatg ctcgcgggct tgctatctcg ctgacggaca ggcaaattga tgaccagctt
    50461 ttaaaccgac tccgtcagtc tctggaaccc caccgctctg ggacaattcc agtacatctc
    50521 tactatcaga gggcggatgc acgcgcgcgg ttgcgttttg gcgcgacgtg gcgtgtctct
    50581 ccgagcgatc gtttattaaa cgatctccgt ggcctcattg gttcggagca ggtggaactg
    50641 gagtttgact aatacaggaa tactatgagt ctgaatttcc ttgattttga acagccgatt
    50701 gcagagctgg aagcgaaaat cgattctctg actgcggtta gccgtcagga tgagaaactg
    50761 gatattaaca tcgatgaaga agtgcatcgt ctgcgtgaaa aaagcgtaga actgacgcgt
    50821 aaaatcttcg ccgatctcgg tgcatggcag attgcgcaac tggcacgcca tccgcagcgt
    50881 ccttataccc tagattacgt tcgcctggca tttgatgaat ttgacgaact ggctggcgac
    50941 cgtgcgtatg cagacgataa agctatcgtc ggtggtatcg cccgtctcga tggtcgtccg
    51001 gtgatgatca ttggtcatca aaaaggtcgt gaaaccaaag aaaaaattcg ccgtaacttt
    51061 ggtatgccag cgccagaagg ttaccgcaaa gcactgcgtc tgatgcaaat ggctgaacgc
    51121 tttaagatgc ctatcatcac ctttatcgac accccggggg cttatcctgg cgtgggcgca
    51181 gaagagcgtg gtcagtctga agccattgca cgcaacctgc gtgaaatgtc tcgccttggc
    51241 gtaccggtag tttgtacggt tatcggtgaa ggtggttctg gcggtgcgct ggcgattggc
    51301 gtgggcgata aagtgaatat gctgcaatac agcacctatt ccgttatctc gccggaaggt
    51361 tgtgcgtcca ttctgtggaa gagcgccgac aaagcgccgc tggcggctga agcgatgggt
    51421 atcatcgctc cgcgtctgaa agagctgaaa ctgatcgact ccatcatccc ggaaccgctg
    51481 ggcggcgctc accgtaaccc ggaagcgatg gcggcatcgt tgaaagcgca actgctggcg
    51541 gatctggccg atctcgatgt gttaagcact gaagatttaa aaaatcgtcg ttatcagcgc
    51601 ctgatgagct acggttacgc gtaattcgca aaagttctga aaaagggtca cttcggtggc
    51661 ccttttttat cgccacggtt tgagcaggct atgattaagg aaggattttc caggaggaac
    51721 acatgaacat cattgccatt atgggaccgc atggcgtctt ttataaagat gagcccatca
    51781 aagaactgga gtcggcgctg gtggcgcaag gctttcagat tatctggcca caaaacagcg
    51841 ttgatttgct gaaatttatc gaacataacc ctcgaatttg cggcgtgatt tttgactggg
    51901 atgagtacag tctcgattta tgcagcgata tcaatcaact taatgaatat ctcccgcttt
    51961 atgccttcat caacacccac tcgacgatgg atgtcagcgt gcaggatatg cggatggcgc
    52021 tctggttttt tgaatatgcg ctggggcagg cggaagatat cgccattcgt atgcgtcagt
    52081 acaccaacga atatcttgat aacattacgc cgccgttcac gaaagccttg tttacctacg
    52141 tcaaagagcg gaagtacacc ttttgtacgc cggggcatat gggcggcacc gcatatcaaa
    52201 aaagcccggt tggctgtctg ttttatgatt ttttcggcgg gaataccctt aaggctgatg
    52261 tctctatttc ggtcaccgag cttggttcgt tgcttgacca caccgggcca cacctggaag
    52321 cggaagagta catcgcgcgg acttttggcg cggaacagag ttatatcgtt accaacggga
    52381 catcgacgtc gaacaaaatt gtgggtatgt acgccgcgcc atccggcagt acgctgttga
    52441 tcgaccgcaa ctgccataaa tcactggcgc atctgttgat gatgaacgat gtagtgccag
    52501 tctggctgaa accgacgcgt aatgcgttgg ggattctggg ggggatcccg cgccgtgaat
    52561 ttactcgcga cagcatcgaa gagaaagtcg ctgccactac gcaagcacaa tggccggttc
    52621 atgcggtgat caccaactcc acctatgatg gtttgctcta caacaccgac tggatcaaac
    52681 agacgctgga tgtcccgtcg atccacttcg attccgcctg ggtgccgtat actcattttc
    52741 atccaatcta ccagggtaaa agtggtatga gcggcgagcg tgttgcggga aaagttttct
    52801 tcgaaacgca atcgacccac aaaatgctgg cggcgttatc gcaggcttca ctgatccaca
    52861 ttaaaggcga gtatgacgaa gaggcgttta acgaagcctt tatgatgcat accaccacct
    52921 cgcccagtta tcccattgtt gcttcggttg agacggcggc ggcgatgctg cgtggtaatc
    52981 cgggcaaacg gctgattaac cgttcggtgg agcgggcgct gcattttcgc aaagaggtcc
    53041 agcggctgcg ggaagagtct gacggttggt ttttcgatat ctggcaaccg ccgcaggtgg
    53101 atgaagccga atgctggccc gttgcgcctg gcgaacaatg gcacggcttt aacgatgcgg
    53161 atgccgatca tatgtttctc gatccggtta aagtcactat tttgacaccg gggatggacg
    53221 aacagggcaa tatgagtgag gaggggatcc cggcggcgct ggtagcaaaa ttcctcgacg
    53281 aacgtgggat cgtagtagag aaaaccggcc cttataacct gctgtttctc tttagtattg
    53341 gcatcgataa aaccaaagca atgggattat tgcgtgggtt gacggaattt aagcgctctt
    53401 acgatctcaa cctgcggatc aaaaatatgc tgcccgatct ctatgctgaa gatcccgatt
    53461 tctaccgcaa tatgcgtatt caggatctgg cgcaagggat ccataagctg attcgtaaac
    53521 acgatctttc cggtttgatg ttgcgggcat tcgatacttt gccggaaatg atcatgacgc
    53581 cacatcaggc atggcagaga cagattaaag gtgaagtaga aaccattgcg ctggaacaac
    53641 tggtcggtag agtatcggca aatatgatcc tgccttatcc accgggcgta ccgctgttga
    53701 tgccaggaga aatgctgacc aaagagagcc gcacggtact cgattttcta ctgatgcttt
    53761 gttccgtagg gcaacattac cccggttttg aaacggatat tcacggcgcg aaacaggacg
    53821 aagacggcgt ttaccgcgta cgagtcctaa aaatggcggg ataacttgcc agagcggctt
    53881 ctgggcgagt aacgtgctgt taacaaataa aggagacgtt atgctgggtt taaaacaggt
    53941 tcaccatatt gcgattattg cgacggatta tgcggtgagc aaagctttct actgcgatat
    54001 tcttggtttc acgctgcaaa gcgaagtcta tcgcgaagcg cgcgactcgt ggaaagggga
    54061 tttggcgctt aatgggcaat atgtgattga gcttttctct tttccgttcc cgccggagcg
    54121 ccccagccga ccggaagctt gcggcctgcg tcatctggct tttagcgttg atgacatcga
    54181 tgcggcagtg gcgcaccttg aaagccataa cgtgaagtgt gaagccatcc gtgtcgatcc
    54241 atacacgcaa aaacgcttca ccttctttaa cgatccggac gggctgccgt tggaactgta
    54301 tgagcagtaa ggcttgtcat cgccgcattt gcccggtaac gtgccgggca ttgctactgt
    54361 aaaatcgcac catcatgaca ctcacgctca atagaccact tctcacctca cgccagattc
    54421 tggtggcctt tagcggcggg cttgactcca ccgttctgct gcatcagttg gtgcagtggc
    54481 ggacggaaaa tccgggcgtc actctgcgcg ctattcatgt gcatcacggt ttaagtgcca
    54541 atgccgatgc ctgggttacg cattgcgaaa acgtctgcca acagtggcag gtgccgctgg
    54601 tggtcgaacg cgtacaactt gcgcaagagg ggctggggat tgaggcccag gcgcggcagg
    54661 cgcgttatca ggcatttgcc cgtaccctat tgcccggcga agtgctggtc accgcgcaac
    54721 atctcgacga tcaatgtgaa acctttctgc tggcgctaaa acgcggtagc ggccctgccg
    54781 ggctttcggc tatggcggaa gtttcggagt ttgctggaac acggcttatc cgcccgttgc
    54841 ttgcccgcac gcggggggaa ctggagcagt gggcattggc gcatggttta cgctggattg
    54901 aagacgaaag taatcaggac gactcatacg atcgtaactt tctgcgcctg cgcgtagtgc
    54961 cgttattgca gcagcgttgg ccgcattttg cggaagcaac ggcccgcagc gccacacttt
    55021 gtgctgaaca agagagtctg ttggatgaac tgctggcgga tgatttagca cactgccaaa
    55081 cgtcgcaggg gacgctgcag attgcgccaa tgctggcgat gagtgatgcc cgtcgcgcgg
    55141 cgattattcg ccgctggctg gcagggcaga atgcaccgat gccttcccgc gacgcgctgg
    55201 tgcgcatctg gcaggaagtg gcgctggcgc gggaagatgc ttccccctgt ttacgtttgg
    55261 gcgcctttga aatccgccgc tatcagtcgc aactgtggtg gattaaatcc gtcaccgggc
    55321 aaagcgaaac cattgtgctg tggcagacgt ggctacaacc gctggaatta ccggcggggc
    55381 tgggaaccgt acagcttact gcgggaggcg atattcgccc tccgcgtgca gacgaagcgg
    55441 tcagcgtgcg tttcaaagcg ccaggattgc tgcatattgt cgggcgcaac ggcggacgta
    55501 agctgaagaa aatctggcaa gagttaggcg tgccgccgtg gctacgtgac accacgccac
    55561 tgctgtttta tggagaaacg ctgattgcgg cggcaggggt atttgtgacg caagaaggtg
    55621 tggctgaagg tgagaatggc gtaagttttg tctggcagaa aacgcttagt taagtgaaag
    55681 ccggataaga cgcatcaaac gtcgcatccg gcgaaagtca atcaggattc gcttaccacc
    55741 accgtaccga tttcagggtg gctaaagctg gtaattttat ccagacgcag ctcgcgggtt
    55801 tcgccagcgg cctcgacgac cagatactcc acatttttgc gggagactaa atcactggct
    55861 tttgcctgca atttttcgcc atctttcagc tcaagtgtca gcattaaatg atgctggcag
    55921 gcgagctcaa gattatcgta atcatcacaa ttaattggtt gatacgtatc attcattgac
    55981 ataatcgctc accagtaagt ttgccgcagc gtatgctgct ttttccctga cagcctcaga
    56041 aagggcgtcg tcggcagcca tttcattcag cactttcaaa acgcagccca gcgcgtccgg
    56101 aacgtatcct aagtctccgc tggcgatttc cgcgtaccgc ttgcgtatta actcacaata
    56161 tttttccaca tgccctcctg tcagcactct gacttaaccg tggatgcaag tctaagccta
    56221 cgaagataaa ctctgtttcg caaggtgact ataccacact catttctgca atatcagcgc
    56281 cgcaactgca cgtattccgt tacaatggcc tcctgattcg aaaggagttt tcttatggcg
    56341 cttaaagcga caatttataa agcgacggtt aatgtggccg atctcgaccg caaccagttt
    56401 ctcgatgcct ctctgacgct ggcgcgccac ccttcagaaa cccaggagcg tatgatgctg
    56461 cgcttgctgg cgtggctgaa atatgctgat gaacgtctgc aatttacccg tggtttgtgt
    56521 gccgatgatg agccggaagc gtggctgcgt aacgatcacc tgggcattga tttgtggatt
    56581 gagctggggc tgccggatga gcgacgaatt aagaaagcct gcacccaggc cgcagaagtg
    56641 gcgctgttta cctataatag tcgggcggcg caaatctggt ggcagcaaaa tcagagcaaa
    56701 tgtgtgcagt ttgccaatct ttccgtctgg tatctggacg atgaacaact ggcgaaagta
    56761 agcgcctttg ccgatcgtac catgacgctg caggcaacga ttcaggatgg tgttatctgg
    56821 ttatcggatg ataagaataa tctggaagtt aacttaaccg cctggtaaca accttcatga
    56881 ttgtgatttc ccgacatgtt gctattcccg atggtgagct tgagatcacc gccattcgtg
    56941 cgcagggcgc tggcgggcag catgttaata agacctcaac ggctattcat ctgcgttttg
    57001 acattcgggc gcccagcctg ccagagtatt acaaagagcg tctgctcgcc gccagccatc
    57061 atttgatcag cagtgatggc gtgattgtca ttaaggcaca ggaataccgc agtcaggaac
    57121 tgaaccgcga agccgctctg gcccggttgg tggcagtgat taaagattta acaaaagaac
    57181 aaaaagcccg acgacccacg cggcccaccc gtgcatcgaa agagcgcagg ctggcatcga
    57241 aagcacaaaa atcaagcgtg aaggcgatgc gcggcaaagt acgcagcggt cgggaataaa
    57301 aagaaggaat ggatggtgaa aaaagcgata gtgacagcga tggctgtaat cagcctcttt
    57361 actctgatgg gatgtaataa tcgggccgaa gtcgatacgc tttctccggc gcaggctgcc
    57421 gaactgaaac cgatgccgca aagttggcgc ggcgtgctac cgtgtgccga ttgcgaagga
    57481 atcgaaatct ctctgttcct cgaaaaagac ggaacatggg tgatgaatga gcgttatctc
    57541 ggtgctcgtg aagaaccttc ctccttcgct tcctacggta catgggcgcg aaccgctgac
    57601 aagctggtat taaccgacag caaaggtgaa aagtcatatt atcgggcgaa aggcgatgcg
    57661 ctggagatgc tcgatcgtga aggcaatcca attgaatcgc agttcaacta tacgctggaa
    57721 ccggcacaat ccagtttacc tatgacgccg atgaccctgc gaggcatgta tttttatatg
    57781 gctgatgcgg cgaccttcac tgattgcgcg accggaaaac gtttcatggt agcgaataac
    57841 gcagagctgg agcgtagcta cctggctgcg cgcggtcaca gtgaaaaacc ggtgttactg
    57901 tcagtagaag gtcactttac gcttgaggct aatccggata ctggtgcgcc aaccaaagta
    57961 ttagcgcccg atacggcggg taaattttac ccgaataagg attgcagtag tttggggcag
    58021 taaacctgct taaggcagaa acaaacgcaa aattgcctga tgcgctgcgc ttatcaggcc
    58081 tacagcatat aatgcaacat tttgaattag cacgattttg taggccggat aaggcgttta
    58141 cgccgcatcc ggcattttgt gcacgaggct ggctttatca ctgagtcagc ccaacaaagc
    58201 gacccgcctt gatataaatc ccaggcttca ggtgcccaac atactgtaac tgcgtttctg
    58261 gcttaaacgc agagacatca ccaatacgca tatgcatcag atcggcaggg ctaatccagc
    58321 ccgattgcgc cagtgtaagt ggatgcccgg ctttggcaaa tgcatcagtg acaaattccg
    58381 aacaaaacca cgactttttg tctccttcgc acacactgct taactgcgct ttcgccaggt
    58441 cgctgacgca ctgttggcga aaatcctcgg agaacggatt cagtgagcac atctggcgag
    58501 tcaccataaa gggaataaat tcgacaatgt tgcgatagtt ataaccgcta tctttgattt
    58561 tattggcaaa cgcggtgata tctgtggctt gttgcggggt aagatccggg actcgtaaga
    58621 cgaaaagctt atcactatgc ttcatcgctt ttttaaggga aacaatctgg acgccagcgc
    58681 ctgtcgcttc tgcaacgtta ttatcaccca gaaagattgc aacgtgactc acagaggaag
    58741 tgctgaagac gcggattcca aatgaggtta ccccaaggct tgaggagaac agcaaatcgc
    58801 cgggtttgag atcaggtgcc gttatttctt ttattgattg ttcggtgaaa gagctttgat
    58861 gctggaattt gacagcccat gtttttgcct cggcatctac tgctgtggct gatgggtctg
    58921 gctggctgat atcaacggta caagccgaaa gtaaaagaaa gctggggaga agcaggcggc
    58981 agtacgcctt tggtttatcc attttataca atccatgtaa aaaagggccc tgaaattcag
    59041 gaccctttct ggcatcagcc tttaatctgt ttcaccagat attcgacgat gtcaccagtc
    59101 ttaattaact gtttctcgcc gttgcgacga tatttatatt cgatatcgtc gttgtcgagg
    59161 ttacggtcgc ccagcacaat agtgtgcgga ataccgatca gttccatatc agcaaacatc
    59221 acgcccggac gctctttgcg gtcatccagc agcacttcga taccttgtgc acgcagttcg
    59281 ctgtacagtt tctcagcaag ctcttgtacg cggaaggatt tgtgcatgtt catcggcaga
    59341 atcgccacct ggaacggcgc gatagcgtca ggccatacga tgccgcgttc gtcgtagttc
    59401 tgctcaatcg ccgcagctac cacacgcgtt accccgatac cgtagcaacc catcgtcagg
    59461 atttggttac ggccatcttc accctgtacg gaggctttca gtgcttcgga gtacttggta
    59521 cccagctgga agatgtgacc aacttcgata ccacgtttga tcagcagcgt accctggcca
    59581 tccgggcttg gatcgccagc caccacgtta cggatatctg caacttccgg ggtagcgaca
    59641 tcgcgatccc agttgatacc gaagtagtgt ttaccatcga tgttagcacc agcagcgaaa
    59701 tcactcatcg ccgcaacggt acggtcaatc accaccggaa tcggcatgtt taccggaccc
    59761 agtgaacccg gaccggcttt aaccacagca cgaatttctt cttcggtcgc gaaagtcagc
    59821 gggcttgcaa cctgcggcag tttttctgct ttaacttcgt tcagctcgtg atcaccgcgc
    59881 accagcagcg caaccagcgg gaagctactg ccttcaaccg ctttaaccag cagagtctta
    59941 accgttttct caatcggcag attgaactgt tcaaccagtt ccgcgatggt tttcgcgttc
    60001 ggcgtatcaa ccagcgtcat ttcctgggta gcagcagcgc gcggttcttt cggcgcgata
    60061 gcttctgcca gttcaatgtt cgctgcatag tcagaggtgt cggagaagac cacatcgtct
    60121 tcaccgctct gcgccagcac ctggaattcg tgagaggcgc tgccgccgat agaaccggtg
    60181 tcggcctgta cggcgcggaa atccagcccc atgcggctga agattttgct gtaggccgca
    60241 tacattgcat cgtaggtttc ctgcagggat tcctgagaag tatggaaaga gtaagcatct
    60301 ttcatcagga attcgcggga acgcatgacg ccgaaacgcg gacgcacttc gtcgcggaac
    60361 ttggtctgga tctgatagaa gttcagcggc agctgtttgt aagagctaag ctcgttacga
    60421 atcaggtcag tgataacttc ttcatgagtt gggccgagta cgaacggacg ctcgccacgg
    60481 tcaacaaaac gcagcagttc cggaccgtac tgttcccaac gaccactctc ttgccacaaa
    60541 tcggctggct gaaccaccgg catcgacacc tcgatcgcac cggcgttgtt catctcttca
    60601 cgcacgatgt tttcgacttt tttcagaacg cgcacgccgg tcggcagcca ggtatataac
    60661 ccggaggcca gcttgcggat catcccggcg cgcagcatca gctgatggct gatcacctcg
    60721 gcgtcggcag gtgtctcctt gagagtggag agcaggtatt ggctagtacg catgttgtta
    60781 cggttccagt tggaaggtag aacaggctca aggcgagcct gggacaaaaa aagtgattta
    60841 gtttaccagt gcaaaagaaa tgtcaaaaga gaagggcgtg aatttaacgc ggttccagcg
    60901 caaagacttc aaaacctgcg tcggtgacgc gccagcgaac gttaaaatca tgtagccaga
    60961 cggcataggt tttgcccgtt tcctcacctt tacgataggc cgggcgcggg tcctgcgcca
    61021 gtacttcgcg gataaacagc gttaactgcg gataacgctt ctccagcgtc aaaagctgct
    61081 tttcgacctc tgcggtaaaa ctcaccgcca tctctgcagc tggcgcactt tgcgcatagc
    61141 tggcactggc atcgggaagc gattcggcaa aggggagata cggtttgata tccactaccg
    61201 gcgtaccatc gaccagatcc agactgccga gcttcagaat cacgctgtct ttatggcaaa
    61261 caacctcttt cagctctacc agcgacatgc caattgggtt agggcggaaa gtagagcgtg
    61321 ttgcgaaaac ccccattctg gcgttaccgc cgaggcgcgg tggacgcaca gtcggacgcc
    61381 agccgccttc catcgtttga tgaaagacga aaaggatcca taaatggctg aatgcttcca
    61441 ggccgcgaac ggcgtcggcc tggttgtagg aagcaatgag atgcagttcg ccgttggcgc
    61501 tttttaccag acctggctgg cgcggaacgg cgaatttttc tttatagggc gagcgaataa
    61561 cgcctatttg ctcgaactgg aaactgctca tttcgccgta atgttaagcg cagagccgat
    61621 acatacagcc tgacgatagc agcccggcgt accgctggtg acttcgcagc tatgcagtaa
    61681 tacagcattg gctttcattt tagaggcatt gatttgcatc tgcttacgtg cggttggaat
    61741 gctcggcgga gagtcctgat tagaggcctg gcaagagtcg ccactgactt caccgagatc
    61801 gcggaacggt ttgccgacta attcttctgc attggtataa attcggaccg gcgtggcgcg
    61861 cggcgctttc ggttttgcag gctccgcttt cggctggggt gcagtgcttt gaacgggttc
    61921 gacaggggat ctgcttaaca tggaacagcc gcttagcatg agtgctacta aacagatcgg
    61981 taaagcacgc atagtatttc ctcaatgtat gatcaaaacg tcaatattga atcaggagct
    62041 tgtaaaaatg acaagacggg caagcgcccg tcctgaatga tattacaaat tgtggaaaca
    62101 gcctaaaaat taccagcctt taacagcgcc gccgttaaac actttgtttg ctgcttcgta
    62161 aacttcatca gactgataag cctggacgaa tttcttcacg ttctcggcgt ctttgttatc
    62221 ttcacgcgtt acgatcaggt ttacgtatgg ggagtcttta tcttcgacaa agataccgtc
    62281 tttcgccgga gtcaggccaa tctggctggc ataggtggta ttgataactg ccagagcgat
    62341 ttgcgcgtcg tccagagaac gcggcagttg cggtgcttcc agttcaacaa ttttcagatt
    62401 ttttgggttc tcaacaacat caagaactgt cggcagcagg ccaacgccat ctttcagttt
    62461 gatcaagccc actttttgca gcagcagcag tgaacgacca agattagttg gatcgtttgg
    62521 cacggcaacc tgcgaaccat cctgcagttc atccagtgat ttgattttct tggagtaacc
    62581 agcaatcgga taaacaaaag tgttgcctac tgcgaccagt ttgtagccac gatctttcag
    62641 ttgctgatca aggtacggtt tatgctggaa ggcgttggcg tcgatatcgc ctttgctcaa
    62701 tgcttcgttt ggcagaacat agtcgttgaa ggttaccagc tcaacgtcca ggccatattt
    62761 gtctttcgca actttctgcg cgacttctgc tacctgctgt tcggcaccaa caatcacgcc
    62821 gactttaatg tggtttggat ctttttcatc ctgaccgcag cctaccagtg ccagtgatcc
    62881 aatcagggct cccactgccg caaaggtttt gaatttgaac gccatacctt attccttctt
    62941 caattattta tgttgtgttg aacgttactt gcgagtgaca gcccggacga tgcggtcgcc
    63001 tgcgaactga attaaataaa ccagaatgac cagcaatacc agtaccgtat tcatcaccgt
    63061 cgcgttgtag ccgatgtagc catactgata gccaatctga cctaaaccac cggcaccgac
    63121 tgcaccaccc atcgcggaat aaccgaccag ggtaatcagg gtgatagttg ccgcattcac
    63181 cagacccggc aacgcttccg gtaacagcac tttacggacg atctgcattg gcgtggcacc
    63241 cattgcgcgg gaagcttcaa ttaacccggt tgggatctcc agcagagcgt tctcgaccat
    63301 acgggcaata aacggtgctg caccaacggt taacggaaca attgctgcct gcaaaccaat
    63361 cgatgtaccg acaataacgc gggtaaacgg aatcatccat accagcaaga taatgaacgg
    63421 gatggaacgg aaaatgttca caatcgcaga aacggtacga tacagcttcg cgttagcaat
    63481 aatttgcccc ggacgcgtga cataaagcag aacgccaacc ggcagaccaa tcacaaaccc
    63541 aaaaaatccg gatacgaagg tcattgccag cgtttcccat acgccacgaa ccagcagcca
    63601 catcatcggc tcagacataa cccagtacct ctacttttac atggtgttcc tgcagccagg
    63661 caatggcggc ttgcgtatct tgttgtgtgc cgtgcatttc agtcagcatg atgccgaatt
    63721 taacgccacc ggcgtagtcc atctgcgcgc taataatgtt gttgttgacg ttgaaacgac
    63781 gcgcggtttc agaaagcagt ggggcatcga ccgattgacc ggtaaactcc agacgcagca
    63841 tcggcacgca gtcagtaaat ggctccgttt gcagacgttc ctggtaatct tccgggatat
    63901 ccagatgcag ggtcgactga ataaacttct gcgccagcgg cgttttcgga tgcgagaaca
    63961 cttcacttac cgtgtcctgc tcgatcagtt cgccattgct gatgaccgcc acgcaatcac
    64021 aaatacgctt cacaacgtcc atttcgtggg tgatcaacag aatcgtcaaa cccagacggc
    64081 ggttaatgtc tttcagcagt tcgagaatag aacgtgtcgt tgccgggtcc agcgcgctgg
    64141 tggcttcatc acacagcaat actttgggat tgctggctaa tgcacgggca atcgccacac
    64201 gttgtttctg cccaccggaa agattcgacg ggtagctatc atgcttatcg ccaagaccaa
    64261 ccaatgacag caattccgtc acgcgacgtt tgatctcgtc tttcggtgtg ttgtccagct
    64321 ccagcggcag agccacgttg ccaaaaacag tacgcgaaga gagcaggtta aaatgctgga
    64381 aaatcatacc aatctggcgg cgagctttgg tcaactcgga ttctgacagc gtggtcaatt
    64441 cctggccatc gaccagcacg ctaccctcgg ttgggcgctc cagcaggttt acacaacgta
    64501 taagcgtact cttacccgcg cctgaggcac cgataacgcc ataaatttgt ccagctggca
    64561 catgcaggct gacgttgttc aacgcctgga tggtgcgggt gccctggtgg aacactttgg
    64621 tgatattcga aagttttatc attgattatt tattatcgtc attaagttag tcgtagcatc
    64681 tcgaatgcct gaaacgggca acgccgtcaa tgaaatggat gttaaggcat ccagacgtct
    64741 aaatcaatca ggtttatgcg aagagcactt tcttgcaggt cgaaacatgc gatactagcg
    64801 tcccatgcct tattaaggag ctataaaagg tggcgaagag cgtacccgca atttttcttg
    64861 accgtgatgg caccattaat gtcgatcacg gctatgtcca tgagatcgac aactttgaat
    64921 ttatcgacgg tgttattgac gccatgcgcg agctaaaaaa aatgggcttt gcgctggtgg
    64981 tagtaaccaa ccagtctggc attgctcgcg gtaaatttac cgaagcacag tttgaaacgc
    65041 tgaccgagtg gatggactgg tcgctggcgg accgagatgt cgatctggat ggtatctatt
    65101 attgcccgca tcatccgcag gggagtgttg aagagtttcg ccaggtctgc gattgccgca
    65161 aaccacatcc ggggatgctt ttgtcagcac gcgattattt gcatattgat atggccgctt
    65221 cttatatggt gggcgataaa ttagaagata tgcaggcagc ggttgcggcg aacgtgggaa
    65281 caaaagtgct ggtgcgtacg ggtaaaccta ttacacctga agcagaaaac gcggcagatt
    65341 gggtgttaaa tagcctggca gacctgccgc aagcgataaa aaagcagcaa aaaccggcgc
    65401 aatgattaaa agatgaacgg ttgaaataaa aatgcatttt tccgcttgtc ttcctgagcc
    65461 gactccctat aatgcgcctc catcgacacg gcggatgtga atcacttcac acaaacagcc
    65521 ggttcggttg aagagaaaaa atcctgaaat tcaggggtga ctctgaaaga ggaaagtgtg
    65581 atatacgcca cctcgcgaca gtgagctgaa agc
//
LOCUS       DADMGC010000007        59976 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.007, whole genome shotgun sequence.
ACCESSION   DADMGC010000007 DADMGC010000000
VERSION     DADMGC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.007"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..310
                     /locus_tag="JNZ73_000452"
     CDS             <1..310
                     /locus_tag="JNZ73_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8730859.1"
                     /translation="YQYEQRFGMRSTSLLNVIGSNTIAIYTTHRILVEIFSLTLLAQM
                     NAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKLSQRAFSDLLFSPPSLPAAVSYSR
                     "
     gene            438..3884
                     /gene="mfd"
                     /locus_tag="JNZ73_000453"
     CDS             438..3884
                     /gene="mfd"
                     /locus_tag="JNZ73_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAY8730860.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRYVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLNDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGMIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            4027..4989
                     /gene="ldtC"
                     /locus_tag="JNZ73_000454"
                     /pseudo
     CDS             4027..4989
                     /gene="ldtC"
                     /locus_tag="JNZ73_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(5072..5329)
                     /gene="bhsA"
                     /locus_tag="JNZ73_000455"
     CDS             complement(5072..5329)
                     /gene="bhsA"
                     /locus_tag="JNZ73_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HAY8730861.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            5570..6202
                     /gene="comR"
                     /locus_tag="JNZ73_000456"
     CDS             5570..6202
                     /gene="comR"
                     /locus_tag="JNZ73_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HAY8730862.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(6264..6803)
                     /locus_tag="JNZ73_000457"
     CDS             complement(6264..6803)
                     /locus_tag="JNZ73_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY8730863.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(7030..8334)
                     /locus_tag="JNZ73_000458"
     CDS             complement(7030..8334)
                     /locus_tag="JNZ73_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY8730864.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(8735..9277)
                     /locus_tag="JNZ73_000459"
     CDS             complement(8735..9277)
                     /locus_tag="JNZ73_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY8730865.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(9300..10325)
                     /gene="nagZ"
                     /locus_tag="JNZ73_000460"
     CDS             complement(9300..10325)
                     /gene="nagZ"
                     /locus_tag="JNZ73_000460"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAY8730866.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(10336..11160)
                     /gene="thiK"
                     /locus_tag="JNZ73_000461"
     CDS             complement(10336..11160)
                     /gene="thiK"
                     /locus_tag="JNZ73_000461"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HAY8730867.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(11141..11782)
                     /gene="lpoB"
                     /locus_tag="JNZ73_000462"
     CDS             complement(11141..11782)
                     /gene="lpoB"
                     /locus_tag="JNZ73_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HAY8730868.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(11797..12174)
                     /locus_tag="JNZ73_000463"
     CDS             complement(11797..12174)
                     /locus_tag="JNZ73_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HAY8730869.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(12177..12536)
                     /gene="hinT"
                     /locus_tag="JNZ73_000464"
     CDS             complement(12177..12536)
                     /gene="hinT"
                     /locus_tag="JNZ73_000464"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HAY8730870.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            12870..15059
                     /gene="fhuE"
                     /locus_tag="JNZ73_000465"
                     /pseudo
     CDS             12870..15059
                     /gene="fhuE"
                     /locus_tag="JNZ73_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(15119..16552)
                     /gene="ptsG"
                     /locus_tag="JNZ73_000466"
     CDS             complement(15119..16552)
                     /gene="ptsG"
                     /locus_tag="JNZ73_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAY8730871.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(16847..17644)
                     /locus_tag="JNZ73_000467"
     CDS             complement(16847..17644)
                     /locus_tag="JNZ73_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY8730872.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLCDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(17655..18659)
                     /gene="holB"
                     /locus_tag="JNZ73_000468"
     CDS             complement(17655..18659)
                     /gene="holB"
                     /locus_tag="JNZ73_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAY8730873.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWVATLLMDALKRHHGAALVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(18656..19297)
                     /locus_tag="JNZ73_000469"
     CDS             complement(18656..19297)
                     /locus_tag="JNZ73_000469"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAY8730874.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(19287..20309)
                     /gene="yceG"
                     /locus_tag="JNZ73_000470"
     CDS             complement(19287..20309)
                     /gene="yceG"
                     /locus_tag="JNZ73_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HAY8730875.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(20312..21121)
                     /gene="pabC"
                     /locus_tag="JNZ73_000471"
     CDS             complement(20312..21121)
                     /gene="pabC"
                     /locus_tag="JNZ73_000471"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAY8730876.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(21241..22482)
                     /gene="fabF"
                     /locus_tag="JNZ73_000472"
     CDS             complement(21241..22482)
                     /gene="fabF"
                     /locus_tag="JNZ73_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAY8730877.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(22569..22805)
                     /gene="acpP"
                     /locus_tag="JNZ73_000473"
     CDS             complement(22569..22805)
                     /gene="acpP"
                     /locus_tag="JNZ73_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY8730878.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(23016..23750)
                     /gene="fabG"
                     /locus_tag="JNZ73_000474"
     CDS             complement(23016..23750)
                     /gene="fabG"
                     /locus_tag="JNZ73_000474"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HAY8730879.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGSQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(23763..24692)
                     /gene="fabD"
                     /locus_tag="JNZ73_000475"
     CDS             complement(23763..24692)
                     /gene="fabD"
                     /locus_tag="JNZ73_000475"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAY8730880.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(24708..25661)
                     /locus_tag="JNZ73_000476"
     CDS             complement(24708..25661)
                     /locus_tag="JNZ73_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAY8730881.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(25729..26799)
                     /gene="plsX"
                     /locus_tag="JNZ73_000477"
     CDS             complement(25729..26799)
                     /gene="plsX"
                     /locus_tag="JNZ73_000477"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAY8730882.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(26880..27053)
                     /gene="rpmF"
                     /locus_tag="JNZ73_000478"
     CDS             complement(26880..27053)
                     /gene="rpmF"
                     /locus_tag="JNZ73_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAY8730883.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(27105..27626)
                     /gene="yceD"
                     /locus_tag="JNZ73_000479"
     CDS             complement(27105..27626)
                     /gene="yceD"
                     /locus_tag="JNZ73_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HAY8730884.1"
                     /translation="MQKVKFPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            27825..28409
                     /gene="yceF"
                     /locus_tag="JNZ73_000480"
     CDS             27825..28409
                     /gene="yceF"
                     /locus_tag="JNZ73_000480"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HAY8730885.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(28521..29480)
                     /gene="rluC"
                     /locus_tag="JNZ73_000481"
     CDS             complement(28521..29480)
                     /gene="rluC"
                     /locus_tag="JNZ73_000481"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HAY8730886.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            30053..33238
                     /gene="rne"
                     /locus_tag="JNZ73_000482"
     CDS             30053..33238
                     /gene="rne"
                     /locus_tag="JNZ73_000482"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HAY8730887.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(33435..34388)
                     /gene="flgL"
                     /locus_tag="JNZ73_000483"
     CDS             complement(33435..34388)
                     /gene="flgL"
                     /locus_tag="JNZ73_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
                     /protein_id="HAY8730888.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRTELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(34400..36042)
                     /gene="flgK"
                     /locus_tag="JNZ73_000484"
                     /pseudo
     CDS             complement(34400..36042)
                     /gene="flgK"
                     /locus_tag="JNZ73_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(36108..37049)
                     /gene="flgJ"
                     /locus_tag="JNZ73_000485"
     CDS             complement(36108..37049)
                     /gene="flgJ"
                     /locus_tag="JNZ73_000485"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HAY8730889.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDQVSKTYSMNIDNLF"
     gene            complement(37049..38146)
                     /locus_tag="JNZ73_000486"
     CDS             complement(37049..38146)
                     /locus_tag="JNZ73_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAY8730890.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAVVMVTVSLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(38158..38856)
                     /gene="flgH"
                     /locus_tag="JNZ73_000487"
     CDS             complement(38158..38856)
                     /gene="flgH"
                     /locus_tag="JNZ73_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAY8730891.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(38909..39691)
                     /gene="flgG"
                     /locus_tag="JNZ73_000488"
     CDS             complement(38909..39691)
                     /gene="flgG"
                     /locus_tag="JNZ73_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAY8730892.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(39829..40580)
                     /locus_tag="JNZ73_000489"
                     /pseudo
     CDS             complement(39829..40580)
                     /locus_tag="JNZ73_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(40600..42519)
                     /gene="flgD"
                     /locus_tag="JNZ73_000490"
                     /pseudo
     CDS             complement(40600..42519)
                     /gene="flgD"
                     /locus_tag="JNZ73_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(42531..42934)
                     /gene="flgC"
                     /locus_tag="JNZ73_000491"
                     /pseudo
     CDS             complement(42531..42934)
                     /gene="flgC"
                     /locus_tag="JNZ73_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(42938..43111)
                     /locus_tag="JNZ73_000492"
     CDS             complement(42938..43111)
                     /locus_tag="JNZ73_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgB"
                     /protein_id="HAY8730893.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVTQFADNSLQYQMSLSALSG
                     QIKGMMNVLQSGN"
     gene            43266..43925
                     /gene="flgA"
                     /locus_tag="JNZ73_000493"
     CDS             43266..43925
                     /gene="flgA"
                     /locus_tag="JNZ73_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAY8730894.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            44001..44277
                     /gene="flgM"
                     /locus_tag="JNZ73_000494"
                     /pseudo
     CDS             44001..44277
                     /gene="flgM"
                     /locus_tag="JNZ73_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
     gene            44282..44698
                     /gene="flgN"
                     /locus_tag="JNZ73_000495"
     CDS             44282..44698
                     /gene="flgN"
                     /locus_tag="JNZ73_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HAY8730895.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDHEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(44738..46273)
                     /gene="murJ"
                     /locus_tag="JNZ73_000496"
     CDS             complement(44738..46273)
                     /gene="murJ"
                     /locus_tag="JNZ73_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAY8730896.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSHLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(46440..47306)
                     /locus_tag="JNZ73_000497"
     CDS             complement(46440..47306)
                     /locus_tag="JNZ73_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY8730897.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(47308..47955)
                     /locus_tag="JNZ73_000498"
     CDS             complement(47308..47955)
                     /locus_tag="JNZ73_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HAY8730898.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTLGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(47966..48550)
                     /gene="rimJ"
                     /locus_tag="JNZ73_000499"
     CDS             complement(47966..48550)
                     /gene="rimJ"
                     /locus_tag="JNZ73_000499"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HAY8730899.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            48786..49994
                     /gene="mdtH"
                     /locus_tag="JNZ73_000500"
     CDS             48786..49994
                     /gene="mdtH"
                     /locus_tag="JNZ73_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HAY8730900.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEYR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSTHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            50058..50705
                     /gene="grxB"
                     /locus_tag="JNZ73_000501"
     CDS             50058..50705
                     /gene="grxB"
                     /locus_tag="JNZ73_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HAY8730901.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYHDNMAKQTQINLLSSMAI"
     gene            50839..51399
                     /locus_tag="JNZ73_000502"
     CDS             50839..51399
                     /locus_tag="JNZ73_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8730902.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            51505..52551
                     /gene="pyrC"
                     /locus_tag="JNZ73_000503"
     CDS             51505..52551
                     /gene="pyrC"
                     /locus_tag="JNZ73_000503"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAY8730903.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLQTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            52625..52870
                     /gene="dinI"
                     /locus_tag="JNZ73_000504"
     CDS             52625..52870
                     /gene="dinI"
                     /locus_tag="JNZ73_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HAY8730904.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            53160..53414
                     /gene="bssS"
                     /locus_tag="JNZ73_000505"
     CDS             53160..53414
                     /gene="bssS"
                     /locus_tag="JNZ73_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HAY8730905.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            53529..54647
                     /gene="solA"
                     /locus_tag="JNZ73_000506"
     CDS             53529..54647
                     /gene="solA"
                     /locus_tag="JNZ73_000506"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HAY8730906.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            54695..54808
                     /locus_tag="JNZ73_000507"
     CDS             54695..54808
                     /locus_tag="JNZ73_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HAY8730907.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            55069..55635
                     /locus_tag="JNZ73_000508"
     CDS             55069..55635
                     /locus_tag="JNZ73_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAY8730908.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            55639..56214
                     /locus_tag="JNZ73_000509"
     CDS             55639..56214
                     /locus_tag="JNZ73_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAY8730909.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(56256..57308)
                     /locus_tag="JNZ73_000510"
     CDS             complement(56256..57308)
                     /locus_tag="JNZ73_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAY8730910.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            57533..58453
                     /locus_tag="JNZ73_000511"
     CDS             57533..58453
                     /locus_tag="JNZ73_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HAY8730911.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            58625..58822
                     /locus_tag="JNZ73_000512"
     CDS             58625..58822
                     /locus_tag="JNZ73_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HAY8730912.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            58846..>59976
                     /locus_tag="JNZ73_000513"
     CDS             58846..>59976
                     /locus_tag="JNZ73_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8730913.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIKCSECGATIVLGGYALKRFGMYRCPNRSANR
                     CSAKAISRKQTDATILYMLALCDRFETENTDTIDSLKLQREDL"
BASE COUNT        14651 a        15902 c        14886 g        14537 t
ORIGIN      
        1 ttatcagtac gagcaacgat tcggtatgcg ctccaccagc ctgctgaatg tgattggctc
       61 caacaccatt gctatctaca ccacccatcg cattctggtt gaaatattca gcttaactct
      121 gcttgcgcaa atgaacgcag cacgctggtc gccgcaagtc gaactgacac tcctgctggt
      181 ttatcccttt gtcagtttgt tcatctgtac tgttgcgggc ttgctggtaa gaaaactttc
      241 acagcgtgca ttcagcgatc tgttgttctc cccgccttca ctgcccgctg ccgtcagtta
      301 ctctcgttaa accatcaccc cagccggggc gattcaattt gctaaaccat gtcgattacg
      361 gataatgaac agcattgctt atcagagata tgcccccata tgttgaggca tatcctaacg
      421 agaatctgac aaccgttatg cctgaacaat atcgttatac gctgcccgtc aaagcgggtg
      481 agcagcgtct gctgggcgag ttaaccggcg cagcctgtgc aacgctggta gcggaaattg
      541 ccgaacgtca cgccggtccg gtggtactca ttgcaccaga tatgcaaaat gctctgcgtt
      601 tgcatgatga aatcagccag ttcaccgatc agatggtgat gaatctggcg gactgggaaa
      661 ctcttcccta cgacagtttt tcgcctcatc aggacattat ctcctcgcgc ctttccaccc
      721 tttaccagct accgacgatg cagcgtggcg tactgattgt tccggtgaat acgcttatgc
      781 agcgcgtttg cccacacagt tttctccacg gtcatgcgct ggtgatgaaa aaaggtcagc
      841 gcctgtcacg agatgcatta cgaacccaac tggacagcgc cggttatcgc tatgttgacc
      901 aggtgatgga gcacggcgaa tacgccacgc gcggcgcgtt gctggatctc ttcccgatgg
      961 ggagtgagct gccttatcgt cttgatttct ttgatgatga aatcgacagc ctgcgggtgt
     1021 ttgacgtcga cagccagcgc acgctggagg aagtagaagc gatcaatctg ctgcccgcac
     1081 acgaatttcc gaccgataaa gcggcaattg aactgttccg cagccagtgg cgcgatacct
     1141 tcgaagtgaa gcgcgatccg gaacatattt accagcaagt gagtaaaggc acattacctg
     1201 ccgggatcga gtactggcag ccattgttct tcagcgaacc actgccgccg ctgttcagtt
     1261 atttccctgc caataccttg ctcgtgaata ctggcgatct ggaaaccagt gccgaacgtt
     1321 tccaggctga cacgctggcg cgttttgaga atcgcggcgt cgatccgatg cgcccgctgt
     1381 tgccaccaca atcgctctgg ctgcgggtgg acgagctctt ctcagagctg aaaaactggc
     1441 cccgagtgca gctaaaaact gaacatttac cgacaaaagc cgcgaatgcc aatttaggtt
     1501 tccagaaact gccagacctg gccgttcagg cgcaacaaaa agcgccgctg gatgcgctgc
     1561 gtaagttcct cgagactttc gacggtccgg tggtgttctc ggtagaaagt gaaggtcgcc
     1621 gtgaagcgct gggtgaactg ctcgcacgaa ttaaaattgc tccgcaacgc attatgcgtc
     1681 ttgatgaagc cagcgaccgt gggcgttatc tgatgattgg cgctgccgaa catggttttg
     1741 tcgataaggt gcgtaatctg gcgctgatct gcgaaagcga tctgctcggt gaacgtgttg
     1801 cccgccgtcg tcaggattct cgccgcacca tcaaccccga tacactgatc cgtaaccttg
     1861 cggagctgca tattggtcag ccggtggtcc atctggagca cggcgtcggt cgctatgccg
     1921 gaatgaccac gctggaagct ggcggcatta ctggcgagta cctgatgctc acctatgcca
     1981 acgacgccaa actgtatgtt ccggtgtcgt cactgcatct gattagccgt tacgcaggtg
     2041 gcgcggaaga aaacgccccg ctgcataaac ttggcggcga tgcgtggtca cgcgcgcggc
     2101 agaaagcggc ggaaaaagtg cgtgatgtgg cggcggaatt gctggatatc tacgcgcaac
     2161 gcgccgccaa agagggcttc gcgtttaaac acgatcgtga gcagtatcag ttgttctgcg
     2221 acagcttccc gtttgaaacc acgccggatc aggcgcaggc cattaatgcg gtacttaacg
     2281 acatgtgtca gccgctggca atggatcgtc tggtgtgcgg cgatgttggc tttggtaaaa
     2341 cagaagtggc gatgcgcgcc gctttcctgg cagtagataa ccacaagcag gtagcggtgt
     2401 tggtgcctac cacccttctt gcgcagcagc attacgacaa cttccgcgac cgtttcgcca
     2461 actggccggt acgtatcgaa atgatctccc gtttccgcag cgccaaagag cagacgcaaa
     2521 tccttgcgga agtggcggaa gggaaaatcg atattctgat cggtacgcac aaactgctgc
     2581 aaagtgacgt caagtttaaa gatttaggcc tgctgattgt cgatgaagaa caccgcttcg
     2641 gggtgcgtca taaagagcgc attaaagcga tgcgcgcgaa cgtggatatt ctgacgctta
     2701 ctgcaacgcc gatcccacgt acgctgaata tggcaatgag cggaatgcgt gacctgtcga
     2761 ttatcgccac gccgcccgcc cgtcgtctgg cagttaaaac ctttgtccgt gagtatgaca
     2821 gcctggtggt ccgggaggcg atcctgcgtg aaattttgcg cggaggacag gtttattatc
     2881 tctacaatga tgtggaaaac attcagaaag ccgccgaacg gctggcagaa ctggtgcctg
     2941 aagcacggat tgccatcggt cacgggcaga tgcgcgagcg cgaactggaa cgggtgatga
     3001 atgatttcca tcatcaacgt ttcaacgtgc tggtttgtac caccattatc gaaaccggga
     3061 tcgacatccc gacagccaac accattatca ttgaacgtgc ggatcacttc ggtctggcgc
     3121 agctgcacca gttacgcggt cgcgtcggac gttcacatca tcaggcatat gcatggctgc
     3181 tgacgccgca tccaaaagcg atgactaccg atgcacaaaa acgtcttgaa gcgattgcct
     3241 cgctggaaga tctcggggca ggttttgcgc tggcaacgca cgatctggag attcgcggcg
     3301 cgggtgaact gcttggcgaa gaacaaagcg gctcaatgga aaccatcggt ttctcgctgt
     3361 atatggagtt gctggaaaac gccgtcgatg cactgaaagc cggacgcgag ccgtcgctgg
     3421 aagatctcac cagccagcaa acagaagtcg agctgcggat gccgtcgcta ttgccagatg
     3481 atttcatccc tgacgtgaac acgcgtctgt cgttctacaa acgtattgcc agcgccaaaa
     3541 cggaaaacga actggaagag atcaaagtcg agcttatcga tcgcttcggc ctgctgccag
     3601 atccggcgcg taccctgctg gatattgccc gtctgcgcca gcaagcgcag aaactgggga
     3661 tcaggaagct ggaaggtaat gagaaaggcg ggatgatcga atttgccgag aagaatcacg
     3721 ttaatccggc ctggttgatt ggtttgctgc aaaaacagcc gcagcattac cgccttgatg
     3781 gtccgacgcg cctgaaattt attcaggatt tgagtgagcg gaaaacgcgt atcgaatggg
     3841 tacgccagtt tatgcgtgaa ctggaagaga acgcgatcgc ttaatgtaaa tcgggggcgt
     3901 ttctgcgccc ccatctgcaa catttacaaa ttctttgcac tttcctgcac tatccgacac
     3961 tgtcaccatc cataattcag gcttatctgt ttattacaat aaccttatat ttattatgga
     4021 tttttggtga tgatcaaaac gcgtttttct cgctggctaa cgttttttac gttcgccgct
     4081 gccgtggcgc tggcgctacc ggcaaaagcc aacacctggc cgctgccgcc agcgagcagt
     4141 cgtctggttg gcgaaaacaa atttcatgtg gtggaaaatg acggtggttc tctggaagcc
     4201 atcgccaaaa aatataacgt cggctttctc gctctgttac aggctaaccc cggcgttgat
     4261 ccttacgtac cgcgcgcggg cagcgtgtta acgatcccgt tgccaaccct acttccagac
     4321 gcgccgcgcg aaggcattgt gatcaacatt gcggagctgc gtctctatta ctacccgccg
     4381 ggtaaaaatt cggtaaccgt gtatccaata ggtattggtc agttaggtgg tgacacgctg
     4441 acaccgacaa tggtgaccac cgtttcagac aaacgtgcaa acccaacctg gacgccaacg
     4501 gcaaacatcc gcgcccgtta taaagcacag ggaattgagt tgcctgcggt agtgccggct
     4561 ggaccggata acccaatggg ccatcatgcg attcgtctgg cggcctatgg cggcgtttat
     4621 ttgcttcacg gtacgaacgc cgatttcggc attggcatgc gggtaagttc tggctgtatt
     4681 cgtctgcggg atgacgatat caaaacactc tttagctagg tcaccccagg caccaaagtg
     4741 aatatcatca acactccgat aaaagtctct gccgaaccaa acggtgcgcg tctggttgaa
     4801 gtacatcagc cgctgtcaga gaagattgat gacgatccgc agctgctgcc aattacgctg
     4861 aatagcgcaa tgcaatcatt taaagatgca gcacaaactg acgctgaagt gatgcaacat
     4921 gtgatggatg tccgttccgg gatgccggtg gatgtccgcc gtcatcaagt gagcccacaa
     4981 actctgtaaa tcctggataa aaaaaatccc cgcgacacgg catgtcgcgg ggaaaaaatc
     5041 agcagtagca ggcattaatg agggttaatg cttatttata aattactgct gttccatgga
     5101 gggtattcgg accggttaca gaagtaatac ggaaagattt tgcgcccatc tcatccgctt
     5161 tttgcgccag ctgctcttcc agcgatccca gatttgtccc cgcgttagca ctgatagtac
     5221 cgactttttg ttggccttct ggcgttgact gaacttcgac agccgcaaag ttggcaaatg
     5281 acatggagct taaaatcgcc gcagcgatga gggtttttac gtttttcata atagtggcct
     5341 tatgcagatg aatgacgtcg caagaaatta cttaacgatc gataactaaa tgatagatgt
     5401 gatctggatc acatacaaga tattttttat aacaatcatt aattaattta aaaatcttta
     5461 aatttcagtc aaatagaaag caattatttt tatgggatct ggactggtga acggaggtga
     5521 ttatttttat aataaccttt cattcacagt caccaggtac aacggcatca tggcaactga
     5581 ctcaacacaa tgtgtaaaaa aaagccgtgg ccgcccaaaa gtgttcgaca gggatgccgc
     5641 gcttgataag gccatgaaat tgttctggca acacggttat gaagcgactt ctcttgcgga
     5701 cctcgtcgaa gcgaccggag ccaaagcgcc cacgctatac gcggaattta ccaacaaaga
     5761 ggggttattt cgtgccgttc tcgaccgcta tatcgatcgt tttgccgcta agcatgaagc
     5821 acagctgttt tgtgaagaga aaagcgtgga gtctgcgctg gctgactatt ttgctgccat
     5881 cgccaactgc tttaccagca aagacacccc ggcaggctgc ttcatgatca acaactgcac
     5941 caccctctcc ccagattcag gagatatcgc caatacgttg aaatcacgcc atgcgatgca
     6001 agagcgcact ttgcagcagt ttttatgtca acgacaagcg cgcggggaaa tcccgaccca
     6061 ctgtgacgtg acacatctgg cagaattcct taattgtatt attcagggga tgtcgatcag
     6121 cgcacgcgaa ggtgcatcgc tggaaaaact gatgcagatt gccggaacga ctttgcgttt
     6181 atggcccgaa ctggtgaaat aagtcgcaaa aaaagcccct cagcctgagc gaatgagggg
     6241 ccaaattgca gagtacagcc ttgttatact ttgttattca aaatcagttg cccgttacta
     6301 tctagcggga tctgcgtacc cggatcgcgg tccatgcgga ttttgccctg ctgatcgcca
     6361 atcttatagg tcacatcata accgagcatt ttttctgact tgtcatacac cgttttacaa
     6421 cgctgttgcg tagtcgtgta agtatcgctt tcctggagag agccctggat ctggttaccg
     6481 gcatatccac cacccagcgc ccccacaaca gtggcgacat ctttaccgcg accaccacca
     6541 aactgatgcc ctatcacgcc accagcaaca gcgccgagca ccgacccggt aatgcgattt
     6601 tcatcctgca ccggtcgacg atgggtcact gtgacgttgc gacactcctg acgcggtgtt
     6661 ttaaccgttt ccttgattgg ggttgcagaa acaacctgag cgtattgcgg gccccgttca
     6721 aacacgttca gactggccac tgccgctacg cccaacgcag ctgcgacacc aatcccgata
     6781 cccgccaaca ttgatttatt cacgggaaca cctccttctt tgcagttatg ccaatcatgc
     6841 aagcgaagga gaaaagcagc tatcagacag aagatgaaaa gcgcgggaat gactggaaat
     6901 acttaaggat tcggaggagt ctgaaggaat gcctgatgcg acgcttaacg cgtcttatca
     6961 ggcctaccgc tcgtaccatt tgtccagggc ggataaggcg tttatgccac atcagccagt
     7021 gtacgtcgat taatgcaact tcaaacgcgg acggataacg cggttaatac tccccaccag
     7081 catcattaat ccggttttaa agtaaccatg cagcgcaatc tgatgcattc ggtatagcga
     7141 gatatataca aagcgcgcaa ttcgtccttc aatcatcatt gagccgcgcg tcaggttacc
     7201 catcaggcta ccaacggtgg agaagttcga cagcgatacc agcgaaccat gatctttata
     7261 ctgataattt ttcagcggct taccgttcat ctgcgccaga atgttgttca ttgcgcaagt
     7321 cgccatctgg tgtgcagcct gagcacgcgg cggaacaaag cccccttccg gacgcgggca
     7381 tgacgcgcag tcgccaatag cgtaaatgtc tggatcgcga gtggtttgca gcgtcggttc
     7441 caccaccagc tggttgatac ggttagtttc aagaccaccg atatctttca ggaagtctgg
     7501 cgctttgatc ccggctgccc ataccatcag atcagcctca atatattcgc catctttagt
     7561 gtgcaggccg ccttcatcag cactggtgac catggtttgc gtcagcacgc gaacgccaag
     7621 tttcgttagc tcgttgtggg ccgcagcaga gatacgtggc ggtaatgcag gcaaaatacg
     7681 ttctcccgct tctaccagcg ttacgttcag ggcttcgttg gtcaggcctt tgtaaccgta
     7741 gctgtgcagt tgcttgatcg cgttgtgcaa ttcagcggag agttctacac ccgtcgcgcc
     7801 gccgccgaca atcgcaatgt tcactttgcc atttgcgccc aggttggcag agtatttcag
     7861 gaacagattc agcatctcct ggtggaagcg acgcgcctgg tgcgggttat cgaggaaaat
     7921 gcagttctct ttgacgcctg gcgtattgaa atcgttagag gtgctaccca gcgccatgac
     7981 cagggtgtca taggcgattt tacgttccgg aaccaacagt tcacctttct cgtcgcgtag
     8041 ttctgcaata gtgattgttt tcgcttcacg atcaatatca atgacggaac ccaactggaa
     8101 ctggaaacca tgattgcgcg catgggccag atagctcaac gcatcgacgc cttcatcaag
     8161 cgagccagtc gccacttcgt gcagcagtgg tttccacagg tggctgtggt tacgatcgac
     8221 cagcgtaatt ttggcttttt tcttacgtcc cagcttatgc cccagctgtg ttgccatttc
     8281 cagcccacca gcaccgccgc cgacaatcac aatctttttc aatggcgtag tcaacgtgac
     8341 ccccttaaat ttattaacca attgttaatt aatggttatt aacatagctc ttaattaaca
     8401 acaggttaca gcaatgaaaa tgttcttcgc tattgagaat aacatgaatg gtgcattggt
     8461 cataccaaaa ttgatgttaa tcaagttttg ttgcttaaaa gataggcaac tttaccgggg
     8521 gagatcaaaa aatagcgttt attatttgag caaattcgga agagtaatat agggtgaagc
     8581 gtatatcgag ggcgtttggt cgtcctgata agatgcgcag catcgcatca ggcattgatg
     8641 ctcgactgtc ggatgcggcg tggacgcctt atccgactta cagaattcgg agcgcatcag
     8701 gtaccggcaa ggttacctga tgcgacgagg gcatttaccc gagggttttg aacgctttaa
     8761 tgcgctgtaa atgcggggag atattcttga atttgtgcgt ctgctcttcg tcccagacaa
     8821 tctcgtaata atgatgcaac tcttcagatg tccgctggct gttaagtgct tcatcattac
     8881 gcgacaaaat aaccagacaa cgatcgcgat tcttctcgcg gaagttggtc acacacttag
     8941 tggcaatatc ggcatactct tccgggcgat caatcttgcc ttccatgttc tcataaggga
     9001 acaaattagg gttgaagatc acctggcgga tatcgcaaag aaaaccaatc cgttccgccc
     9061 agtatccgcc caggccaacg ccgcaaatta gcggacgctc gtcaacgttc agttgcaaca
     9121 ttttgtccac ttctttaagc agatgctgca tatcatgttt cggatgccgc gtactgtagc
     9181 ttatcaagcg tacatccggg tcaataaact gcaattgtaa gactttctcg tggttacccg
     9241 gactgttaga gtcaaaaccg tgtaaataga taatcatcgc ttcctcacat aagccagggt
     9301 tagtgacctg ctttctcttc ctgccagcgt tcatgtaact gattcagacg ggtgctgatc
     9361 gctttccagc gagccgagtc catcagttcc tgtcgcgaaa atgaaccttt atgatacaaa
     9421 cgtgtaacac gttctgcctt gatcggtgac agattatcta acacgctgac ggccccttta
     9481 cgattattgc agaccaggat catatcgcaa cccgcatcca gtgatgcctg cccgcgttcg
     9541 gcataactgc ccataatcgc ggcaccttcc atcgataaat cgtcagagaa aatcacgccg
     9601 tcaaaaccca gttcctgacg caaaacggtt ttcagccagt agggagaacc gctcgccgga
     9661 cgcggatcaa catcactgta gatcacatgc gcaggcataa tggcgtcgag tttattttcg
     9721 cggattaagg aactgaagac cgacatatct ttagcgcgaa tctccgcttg tggacgtgga
     9781 tcgcacggtg tttctttgtg tgagtctgcc gttactgcac cgtgtcctgg gaagtgtttc
     9841 ccggtcgttt tcattccggc ttcatgcata ccatcaataa accggctggc aattgccagg
     9901 gctttttgtg gatcggcatg ataagaacgc tcgccaatcg ccgcgctgat atgcccgaca
     9961 tccagcactg gcgcaaagct gatatcaata tccatagcga tcatttcgct ggccatcagc
    10021 caacccgcct cttgcgccag tttgccgccc tcttccattc ccaacagcgc agcaaaggat
    10081 tgtgctgccg gtaagcgggt aaaaccttcg cgaaaacgct gcacgcgtcc accttcctga
    10141 tctaccgcca ccaccagatg attgcgcgat gctgcgcgga tctggcgcac cagttcacgt
    10201 aactgggcag gatcatgata gttacgcgta aagagaatca gccctcccac cagcggatgc
    10261 gccagtattt cacgctcttc cgcgtccagt tcgtaacctt cgacatccaa cattactgga
    10321 cccacactgc tctccttatt gttttattaa tagctgccgc caggtgtcat cggccagcct
    10381 gataaattgt tgatcgccgg tttgtcgcca gcggtactca aaccaccctc ctttgagcat
    10441 cagcagccag ggaaaccatc gcctgacctg acgccataat tgcgccggat aaatcttcgc
    10501 gcgagtggca tagtcattga ccaattgccg gtgctgttca gtattttcca cccacaccgc
    10561 cgccagttcc agcgcgatat caccatctcc agcatactcc cagtcgatga gttttaaccc
    10621 tgatgcgcta tgcactaaat ttccggcgtg gacatccata tgcaatggac ttaagcgtaa
    10681 aggtcgtggt tcccgcgctt tgcgcagacg ttttaacatt cgcagccaac ccacagtccg
    10741 ccgcgccgga tcgctttgct gccagtacag ttccagtaag ggcaacagcg ttattcgcca
    10801 gccaaaacgt ggttgttgat gtagataata cagcaagcct gccagttcgt tggtatccgg
    10861 caaatacgtt tttaccgcgc cgggcagata gtcgactacc atccagtcac ggagatataa
    10921 atgcggcttc ggtgcaatgc ttgcgggtag ttgtgataaa gcccgatact ggcgcaagaa
    10981 cgcggactgc ggcgcatcag gatcgtgcgg ctgacgcaca acaaaacgct ggccctgatg
    11041 ttcaatgaga aaactaccgc cactaagccc actattaaac gtcgtgacgg gatgaaactg
    11101 cgggaaaaag cgcgacagca attcgtcgcg cgtgatggga ttattgctgc gaaacggcac
    11161 ctttacctga ccagataatt tcgcccgtct gcaccagcat cagctgcatt tgtagggtcg
    11221 gagcgttaac gttgccagag gcgctggagt acagcacgta atgagcgccg acattgcggg
    11281 caatgcctat ggctttacta cgggtgccta aactgtcctg cggcgacaaa cctaactgtt
    11341 gcttagccat cgacagttgc tgggcggaaa ccagggtaaa tttcccgtta ttagccagcg
    11401 catttcgcag cgtttcggtc gcttctgcgg cattcagcga accgttagta cggttgttaa
    11461 cgctatcaac cagcaggacg ctacccgcag tcaccccgtc agccccaagc atcttactga
    11521 ccatcggctg cattgcgcca ttccagtcat aatggcgaat atgcggcgca ggcggtgccg
    11581 ttcgatcttc gtgctcaatt gggcctggct gctgcgggat cgtcggcacc gagggcactg
    11641 tggggacagg ctgttgtggc tcggctggtt gttccggcgc tggtttcact tcttctaccg
    11701 gtgcaggttc acgttgcccc acacacccgg cgagaaacat cgccagcgcg gtaatcaagg
    11761 cgtagcgact catttttgtc atcaagattc acccctttac aaatatagat aaagtctgac
    11821 tttgtgcgcc cccagaaaat tggcgctgcc gtacagcgtt accgccgaat gtgcgggaat
    11881 ggtaacgctg cgtggccttt ccagaggatg catctccagc cctctggcgt cataccagta
    11941 aaaacgataa tgaacggtaa cgggttcttg cctttcgtta taaagcgttg aggatgctga
    12001 aggttgaata tcagacgtcg aaaggacggg cttttctgca ctgatgcccg cagccaataa
    12061 agatgactcc atcaccagcg attgctcatc attcaccgga atttccggat gtgaacgaca
    12121 gcccaccagc agtaacaaca ccagagacac cagcccaaag catccttttc tcatcgttac
    12181 agacctttat gcgccagcat tggtcccagc ggacggccac ccaacaagtg catatggatg
    12241 tggtaaacct cttgtccgcc atggcggttg gtattcatga tcagacgata gccatcttcg
    12301 gcaatacctt cttgctcagc aatttttgcc gctacggtga tcatgcgtcc cagcgcctgc
    12361 tcatgctcag ctgacacgtc gttcacagtc ggaatgagga tattcggaat gatcagaata
    12421 tgcgttggcg cttgcggcga aatatcgcga aacgccgtta ccagatcatc ctggtagacg
    12481 atatcggagg ggatctcacg acgaataatt ttgctgaata tagtttcttc tgccacgaca
    12541 ttttcctttt tcaaatagcc atgcgttgcg ccatgctacg ccgcgccaca ctgcgagtat
    12601 agagtatggt cgagttaaac ctgctctttc aaccttaact cacgatttct taagcaaaaa
    12661 atcgttttgc tgccgtatat atcgccatta ttcccatttc tgcctgcgaa tgcgctgaaa
    12721 agacattctc tgtttggttt acacgacaaa tatgaatgcg tatatttctc atttgcattt
    12781 gcaagcaaaa ttattcgcac actaaatata accgctcatc accctcctcc ggcatgagcc
    12841 tgacgacaac ataaaccaag agatttcaaa tgctttcaac acaatttaac agggataatc
    12901 aatatcaagc catcaccaaa ccgtcactac ttgccggttg catagcactg gcactattac
    12961 cttctgccgc ttttgctgca ccagccactg aagaaacggt gattgttgag ggttcagcca
    13021 cagctccaga tgatggcgaa aatgattaca gcgtaacgtc tacctctgcg ggtaccaaaa
    13081 tgcagatgac tcaacgtgat attcctcagt cggtcactat tgttagccag cagcggatgg
    13141 aagatcagca gttacaaacg ctgggcgaag tgatggaaaa cacgctgggg atcagcaaaa
    13201 gtcaggcgga ttccgatcgt gctctttatt attcccgcgg attccagatc gataactata
    13261 tggttgatgg tatccccacc tattttgaat cgcgctggaa tctgggcgac gcactttctg
    13321 atatggcact gtttgaacgc gtagaagtag tgcgtggcgc gacaggactc atgaccggga
    13381 cgggtaatcc atctgcggca attaatatgg ttcgaaaaca cgcgaccagt cgtgaattta
    13441 aaggcgatgt ctcggcggaa tacggtagct ggaacaaaga acggtatgtg gcggatttac
    13501 aaagcccact caccgaagac ggtaaaatcc gcgcgcgaat tgtcggcggc taccagaata
    13561 acgactcatg gctggaccgc tacaatagtg aaaagacctt cttctcgggc attgtcgatg
    13621 ctgatttagg cgatcttacg acgctttcag ccggttacga atatcagcgc attgatgtta
    13681 atagcccaac ctagggcggt ttaccgcgct ggaatactga tggcagcagc aacagttacg
    13741 atcgcgcacg cagtaccgca cctgactggg cgtacaacga taaagagatc aacaaggtct
    13801 ttatgaccct gaagcagcgg tttgctgata cctggcaagc gacactgaat gccacccact
    13861 ctgaagtcga atttgacagc aaaatgatgt atgtcgatgc ctatgtaaac aaagcggatg
    13921 gtatgctggt tgggccatac agtaattatg gacctggctt tgatcatgtc ggcggcaccg
    13981 gttggaacag tggcaaacgt aaagttgatg cgctggattt gttcgctgac ggtagttatg
    14041 aattgtttgg tcgtcagcac aatctaatgt ttggtggcag ttacagcaaa caaaacaatc
    14101 gttacttcag ttcatgggcc aacatcttcc cggatgaaat tggcagtttc tacaacttta
    14161 atggcaattt cccacaaacc gactggtcac cacagagcct ggcgcaggac gataccacac
    14221 atatgaaatc gttatatgct gccactcgtg tcacccttgc cgatccgctg catctgatcc
    14281 tcggcgcacg ttataccaac tggcgggttg atacgctgac ttacagcatg gagaaaaacc
    14341 acaccacgcc ttacgctggt ctggtgtttg acatcaatga caactggtcg acctacgcca
    14401 gctatacctc tattttccag ccgcaaaatg atcgtgacag ttcaggcaaa tatctggctc
    14461 caatcaccgg taacaactac gagctgggtc tgaaatcgga ctggatgaat agccgtctga
    14521 ccaccacgtt agccatcttc cgtattgagc aggataatgt cgctcagtcc accggtacac
    14581 ctatccccgg cagcaacggc gaaaccgcct ataaagcggt ggatgggaca gtcagtaaag
    14641 gggtggaatt tgaactcaac ggcgcaatta ccgacaactg gcagctgaca tttggcgcaa
    14701 cgcgctatct tgcagaggat aacgaaggaa acgccgttaa tcctaatctg ccacgcacca
    14761 cggttaaaat gttcaccagc tatcggttgc ctgtcatgcc agagttgaca gtcggcggtg
    14821 gtgttaactg gcaaaatcgc gtgtataccg acaccgtgac accgtatggc accttccgcg
    14881 ccgagcaagg tagctacgcg ctggtggatc tcttcacccg ctaccaggtg acgaaaaact
    14941 tctcgttaca ggggaacgtc aataacctgt tcgacaaaac ctacgatacc aacatggaag
    15001 gttctatcgt ctacggcgca ccgcgtaatt tcagcattac cggcacgtat caattctgaa
    15061 taacacctgt aaaaaaggca gccatctggc tgccttagtc tccccaacgt cttacggatt
    15121 agtggttacg gatgtactca tccatctcgg ttttcaggtt atcggattta gtaccgaaaa
    15181 tcgcctgaac accagaacca gcaaccacta cgcccgctgc gcccagtttc ttcaggccag
    15241 cctgatccac tttagacaca tcagcaacgc tgacgcgcag acgggtaata catgcgtcga
    15301 ggttagtaat gttttcttta ccaccaaatg cagcaaccag agccggtgcc atttcgctgg
    15361 tacctgttgc ttttgcatct tcagtcgcgt cttcacgacc cggcgttttc agatccagtg
    15421 ctttaatcag cacgcggaag atggtgtagt aaacaatcgc ataaccgata ccgacgatcg
    15481 ggaacagcca cagtttgctg ctgttaccag acagaacgat gaagtcgatc agaccgtgcg
    15541 agaacgacgt accgtcacgc attcccagaa gaatacagat tgggaatgcc aggcctgcca
    15601 gaatcgcgtg gatgatgtac aggatcggcg caacgaacat gaagaagaac tcgatcggct
    15661 cggtgatacc ggtcaggaac gaggtcagcg ccgcggagat cataataccg cccactttcg
    15721 cgcggttttc tggtttagca gagtgccaga tagcaattgc ggcagctggc agaccgtaca
    15781 ttttgaacag gaagccacca gacagtttac ccgcagtcgg gtcacccgcc atataacgcg
    15841 gaatgtcgcc gtggaaaacc tgacctgctg cgttggtgta ttcaccaatc tgcatctgga
    15901 aaggtacgtt ccagatgtgg tgcagaccaa acggtaccag acaacgttcg atgaaaccgt
    15961 aaatgccaaa cgcaactacc gggttctggt aagcagccca ctgagagaag gtctggattg
    16021 cagaaccaat cggcggccaa atgaaggaca gcacaacgcc agtaaagatg gcagccaggc
    16081 cagaaatgat cggcacaaag cgtttaccgg caaagaagcc aagatactca ggcagcttaa
    16141 tacggtagaa acggttaaac atgtacgctg caatcgcacc ggagataatc cctccgagta
    16201 cgccagtatc cgccaagtgt ttagaggcga tttcttcagc aggtaaatgc agtaccagtg
    16261 gcgcaaccac ggccatggtt ttaaccatga tgccataggc aacaactgcg gccagcgcgg
    16321 atacgccatc gttattggta aagccgaggg cgacaccgat cgcaaaaatc agtggcatgt
    16381 ttgcaaagac ggaaccgcct gcttctgcca taacatgcga tacaacggcg ggcagccagc
    16441 tgaaattcgc ggaaccgacg cccagcagaa tacctgcgat aggtagtacg gataccggca
    16501 gcatcagcga tttaccgacc ttttgcaggt tagcaaatgc attcttaaac ataattgaga
    16561 gtgctcctga gtataggtgc tttttttacg ttctcacgcg tggcaagggg ggagagcctc
    16621 gccgtgtaca gggcatctaa gcgcccttta tttattacac agagtaaaat aattcagtgc
    16681 caatttgttt gacggctatc acgtttcaac ttcagatcat tggagaagtt gcacattttg
    16741 acaaaacata tgtcacaata tttcaaacgc ggttattcac cgcccaattt tcggcggtga
    16801 actttactcg ttttagccat taattacgag ctttaaaaaa actcattcaa cggatggatt
    16861 gaaggcggga agcgtcgatg tgaaacagac gggcgaagtt atcggtggtt acctgcgcca
    16921 gttcttcaac ggcaacacct ttcaacacag ccatgtattc tgcaacgtca cgaaccatcg
    16981 caggttgatt ctcttttcct cgatgcggta ccggcgcaag gtaaggtgag tcagtttcca
    17041 ccagtaatcg atccaggggg acataacgcg cagcatcgca cagttgctcc gcattacgga
    17101 aggtcacaat gccggaaaag gagatgtaaa atccgagatc cagtaattta cccgccgttt
    17161 ctctgtcctc tgtaaaacag tgtagtacgc caccgcaatc cgtcactttt tcttcgcgca
    17221 gaattgccag cgtatcggca cgggcgtcac gggtatggac gataaccggc ttgttcagtt
    17281 cacgaccaat ctggatatga tggatgaagg actcttgctg acgtacttta gtttccggtg
    17341 tgtaataata atccagcccg gtttcaccca gcgctacaac accctcttct gccgccagac
    17401 ggcgtaaatc ttctacatcg tagggatcat tctggttgag tggatgcacg ccacaagaaa
    17461 atacgacgtt gtcacgttcg cctaccagat cccgcatatg taaataaccc ggtaatgttg
    17521 tggcgactgc cagacaaaat ttcacatcgc gtgcggcggc tttcgccaga acgtcatcca
    17581 cgtccttatg caaagattca taatccagac catcgagatg gcagtgtgag tcgactaaaa
    17641 acatgatgtc tctcttaaag atgaggaacc ggtagcacaa cgcccggttg caggtaatgc
    17701 tcaatacgca gtaaaagatc ggtgatgaga agctcgcggt tgatgcctgt gacagacatt
    17761 aactgttcac gaatgtggca aacatccccc agtatagcct gcaggcgcga gggagaaaga
    17821 tggtttgcca gttcggcgac caggcccggc acatcaacat tggtcaccag cgcagcgcca
    17881 tgatggcgtt ttagcgcatc catcagcaac gttgccaccc agtgtaaacg cgccggagct
    17941 tgttcatgat taagggccgc cagcagcgaa taccagtcgc ccgatggcac gctatatgcc
    18001 aacgcctgac acaatgtttc acgagcctgc cagttatctc cctgaaacaa cgccagtgcc
    18061 gcgccaggcg aaccggcgct taagcgcaat gcggcaagta atgcatcctg tgacattgtc
    18121 acttcgcgtg aaagccaagt cacggcgtac tgttctggag gcggcgcaag gtaatgtaac
    18181 cgacaacgac tacgtaatgt tgccagtaaa cgttcaggct cgcgggtagc caggaaaaac
    18241 caggtttctg ctggtggctc ttcaagcgtt ttcagcaatg cgttagccgc ggcgtcggtc
    18301 agtaaggcag catcggttac ccagacaact ttcgcaccac ctaggcgtgc gtgctcattc
    18361 agcttttcgg tgacctcacg taccgcatca atgcccagcg tatttttccc tttttcggga
    18421 gccagggtgt agtaatcggg atgcgtgcca gcctgcatca actgacatcc acgacagtga
    18481 ccgcaacttt tgtggccctg cggttgttgg cagagtaaat aacggctcag ggcgtagatt
    18541 aaagcatcat cgcccatgcc cggtaacgcc tgaatgagta gcgcatggtg acctcttccg
    18601 gcctgatagc tggctaccag tttttcgaaa tcaggtcgta accatggata ccatctcatg
    18661 cgtccaactc cttcacccag tgggtcacgg tagtgcggat tgcatccatc acggcctcca
    18721 gcggctgggt ggcatcaatg gtatgaatac ttttatcttg tgcagccagt tccagatagc
    18781 gggcgcgggt gcgattaaag aaatcgaaag actcttgctc aatacgatcc agctcgccgc
    18841 gcgcacgcgc gcgttttaag ccaacttccg gggtaacatc gagatagagc gttaagtcgg
    18901 ggcgaaaatc cccgagaaca gcatcacgca gtgttgccag catatgttgg tcaataccac
    18961 gtcctccgcc ctgatacgcc tgagtggaga gatcgtggcg atcgccaatc acccaggtgc
    19021 cgttagccag cgcaggtttg atgaccgttt ctaccagttg aacgcgcgcg gcataaaaca
    19081 tcagaacttc ggctttatcg gtaatgactt catcgcctac cgatttgata tccagcacca
    19141 ggcttcttaa cttttcggca agttgcgtac cgccaggttc ccgagtgaaa accatgtcgc
    19201 ggatacccag ttgctcgagc gtctcaacca ccacattacg cgcggtagtt ttgcctgcgc
    19261 cttccagccc ctcaatgacg atatacttac tgcgcatttt tttccttaag cactttcaga
    19321 taatcctgca cagacttgtt atgactggca agattggtat taaacgtgtg accaccttta
    19381 ccatcggcca caaaatagag atacggcgtt tttgccggat gcgcagcagc cttcagcgaa
    19441 tccgcccccg gcgtagctat cgcacccggc ggcaaaccgg taatggtata ggtgttatac
    19501 gctgtcggcg tttccaggtc tgcacgagaa agtttgccat tataacgctc tcccatcccg
    19561 taaatcacgg ttgggtcggt ctgcaggcgc ataccaatgc gtaaacggtt gataaatacc
    19621 gaggcaacct gatcgcgttc actggcaacg gcggtttctt tttcgataat tgatgccatc
    19681 gtcaccagct gatttttatc tttataaggc agaccgtccg cacgcccttc ccaggcgcta
    19741 tcgaccgctt tcaccatttt cttgtgagct cgcttgagta acgcgacatc ggtggtattg
    19801 gcggtataca tcaaggtgtc tggccagaac caaccttcaa tccactccgg gttttccagt
    19861 tcaagtgcct gcgctacggt ggcgtactta tcgtcactca gcgtgtgctt gatatacgga
    19921 gcctcacgca attgcttgag gtagtcgctc agacgcatcc cttctaccag tcgcagcggg
    19981 aactgtgctt ctttaccgct ttccaacaat ttcagcatct cgcgcacggt catctgcggt
    20041 gtaaagcggt aagtcccggc tttaaagtga gaaagatccg gttcgatacg cagcagccat
    20101 tgaaaaaccc gtgggcgatt gatgatctta tcagcataaa gctgttcacc gagcgccaga
    20161 cgtccggtcc ctggcttcag ggtaaatatc gtctcttctt tgataagcaa tttgctgtcg
    20221 gcaagatggc gaaccttcca gacgcccaca ccagcggcga tacccagtac caccagcaat
    20281 aacaagataa tcaataacac ttttttcatg actaattcgg gcgctcacaa agtggggcta
    20341 aatattcata taacgttgct gacgaaaagg tgacatcgcc acaggcacgt acgggcatca
    20401 ctggcattaa cgcattacaa ataaccatct catctgcctg caacgcctcc tccagcgagg
    20461 cttgcacttc gacaagctga taagaggatt gtgccagtaa acggatacag aattgtcgca
    20521 taatgccgtt aacacctgcc tgatccagtc gcggcgtata aactacattg cccttccgcc
    20581 agaacaaatt agccgcacag cattccgtaa cccacccttc gctgtcaagg accagcgcct
    20641 catcagcgtt tgtctgctca agatgagagc gaatcaatac ttgctcaagc cgattaagat
    20701 gtttaatacc tgcaagatga ggattgcgcc ccagccgcac cgggcttagc gccaacgtca
    20761 tcccttcgtt acgcaaacgg tcgtaatgtg caggataagc cgtaacggag agaatccgcg
    20821 ttgctggtcc gctgttcaat gtgctgtacc ctcgcccgcc actaccgcga ctgatcacga
    20881 ctttcagcac accattttgc tgttctgctg ccagcgtttt catctcttgt tcaagctgag
    20941 gccagaagtc acaggaaatc atcaaccgct gacaagcatc ctgtagtcgc tggatatgcg
    21001 ccgataacaa actgacttta ccgtcgataa ctctggcggt ggtaaaacaa ccatcaccaa
    21061 actgcgttgc ccgatcgctt actgccagcg attcctgctt ataaccgtta attaagaaca
    21121 taccggctcc ttatcagaga attgttagtg tggcagcatg ttcactacgg aacaagtcgg
    21181 aataaaagct aagaaaaaag gcccgcaagc ggacctttta tatgggtgga aaatgacaac
    21241 ttagatcttt ttaaagatca aagaaccatt agtgccaccg aagccgaagg agttacacag
    21301 cgtgtattcc attccgctga cctgacgtgc ttcatgcggt acgaaatcca gatcgcaacc
    21361 ttcatccggg ttatccaggt tgatggttgg cggaacagcc tgatcgcgca gcgccaggat
    21421 ggagtagata gattctactg cacccgccgc acctaacagg tgaccggtca tagatttcgt
    21481 ggagcttacc aacacacggc ttgcagcttc accgaagatg gttttcaccg cctgcgcttc
    21541 agctttatcg ccagccggcg tagaagtacc gtgcgcgtta acgtagccaa tctgactcgc
    21601 ttcaatgcct gcatcacgca gagcatttgc cattgccaga gctgcgcctg cgccattttc
    21661 tggcggtgac gtcatatgat aagcatcgct gctcatacca aagccgacga gttcagcgta
    21721 aattttcgca ccgcgttttt tcgcgtgttc gtactcttca agtaccagca taccggcacc
    21781 atcgcccagt acgaagccat cacgctcttt atcccacggg cggctcgccg cttgcgggtt
    21841 atcattgcgg gtagataatg cacgtgccgc gccaaaacca ccaacgccca gcggcgtact
    21901 ggctttctct gcgccacctg caaccatcac gtcagcatcg ccatacgcga taatacgcgc
    21961 agcatggcca atgttgtgca cgccggaagt acaggcggtc gcgatagaga tgcttgggcc
    22021 acgcaggcca tacatgatag tcagatgacc tgccaccatg ttcacaatcg ttgacggaac
    22081 gaagaatggg ctgatcttac gtggaccacc gttcatcaga gatgtgtggt tttcttcgat
    22141 cagtccgagg ccgccaatcc cggagccaat tgcggcacca atgcgggttg cgttctcttc
    22201 cgttatttca aggccagaat cctgcatggc ctgaacgcca gcgacaattc catattgaat
    22261 gaaggcatcc atcttgcgct gttctttgcg cgagataatg tcctcacagt taaaatcctt
    22321 tactaagcca gcaaatttcg ttgcataggc gctagtatcg aaatggtcga ttaggctgat
    22381 gccactctga ccggcaagca gagctttcca ggtagactct acggtattgc cgacaggaga
    22441 caacatgccc agtccggtca caactacacg acgcttagac acgtttgtcc tccaaggagg
    22501 gaaaaatgat tctagtggga caaaaagata aaactcaggc ggtcgaacga ccgcctggag
    22561 atgttcactt acgcctggtg gccgttgatg taatcaatgg cagcctgaac ggtggtgatt
    22621 ttctcagctt cttcgtccgg aatctcagta tcaaactctt cttccagagc cattaccagc
    22681 tcaacggtgt caagagaatc cgcgcccagg tcttcaacga aagaagcatt gttggtaact
    22741 tcttcctgct taacgcccag ctgttcgccg ataattttct taacgcgttc ttcgatagtg
    22801 ctcatactct taaatttcct atcaaaactc gctttcgcga tggttttcgt agtgtataaa
    22861 atgttgaaaa atttgcaact aaatcccggc aggtcttacc acgattttac gttattttga
    22921 ggccttaccc cccaataacg caaatcattt tgcactaatt attactcata accacgcaga
    22981 ctgcgcgcaa catgagcatt ttgtgcaaat cgcggtcaga ccatgtacat cccgccgttc
    23041 acatgcaaag tttcacccgt gatgtaagct gcttcgtcgg atgccaggaa tgcaaccgcg
    23101 ttggcgattt cctgtgcgcc gccgaggcga cccgcaggaa cctgcgccag gatacccgca
    23161 cgctggtcat cgctcagcgc acgtgtcatg tccgtttcaa taaagcccgg agcaacaacg
    23221 tttacagtaa taccgcgtga cgcaacttcg cgcgccagtg atttactgaa gccgatcaag
    23281 cccgctttcg ccgcagcgta gttggcctga ccgccatttc ccatggtacc aaccacagaa
    23341 ccgatagtga taatacgacc atgacgcttt ttcatcatag cgcgcattac cgcttttgac
    23401 agacggaaaa cagatgaaag gttagtttcg ataatatcgt tccactcttc atctttcatt
    23461 cgcattaaca ggttatcacg agtgataccg gcattattga ccaggatatc cacttcacca
    23521 aattctgcgc gaattttttc cagaacagat tcgatagatg ccgggtcggt cacattcaac
    23581 atcagacctt tgccgttggc acctaaataa tcactgatcg cctgagagcc attttcactg
    23641 gtcgcagtgc caataacttt cgcgccacgg gctgcgagcg tttcagcaat tgcgcggcca
    23701 attccgcggc ttgcaccggt taccagtgcg atttttcctt caaaattcat gattttcctc
    23761 ttttaaagct cgagcgccgc tgccatcgct gaaggttcgt tcagcgccga ggcggtcagg
    23821 gtgtcgacaa tgcgtttcgt caggccagta agcaccttgc ccgggccgac ttcatagaga
    23881 tgttctacgc cttgcgctgc catgtactca acagacttcg tccactgaac cgggttatac
    23941 aactgacgta ccagtgcgtc acggatggca tcaccattgg tttcgcattt cacatcaacg
    24001 ttattcacaa cagaaactgt tggtgcgtta aaggtgattt tcgctaattc tactgccagt
    24061 ttgtcggctg ctggtttcat cagcgcacag tgagacggta cgctcactgg taacggcagc
    24121 gcgcgttttg cgccagcagc tttacaggca gcgccagcac gctcaaccgc ttctttatga
    24181 ccggcaataa ccacctgtcc cggagagtta aagtttaccg gagaaacgac ctgaccttct
    24241 gcagcttctt cacacgcttt cgcaatagac gcatcatcca gaccgatgat tgccgccata
    24301 gcgcccgtgc cttccggtac ggcttcttgc atgaacttac cgcgcatctc aaccaaacgc
    24361 accgcatcag cgaaatcaat cacgccagcg caaaccagcg cggagtattc ccccaggctg
    24421 tgaccggcca tcattgccgg tgctttaccg ccctgctgct gccatacgcg atacagcgca
    24481 acagatgcag tcaacagcgc tggctgggtt tgccaggttt tattcagttc ttcagctggc
    24541 ccctgctggg tcagcgccca caggtcgtag cccagcgccg cagaagcttc agcaaacgtt
    24601 tcttcgacaa ttggatagct cgccgccata tcagccagca ttccaacggt ttgagaaccc
    24661 tgtccaggga acacaaatgc aaattgcgtc atgttttaat ccttatccta gaaacgaacc
    24721 agcgcggagc cccaagtgaa tccaccgcca aaggcttcaa gcagaaccaa ctgccccggc
    24781 ttaatgcgcc cgtcgcgtac agcttcatcc agcgcgcacg ggacagaggc cgcagaggta
    24841 ttaccgtggc gatccagcgt caccacgaca ttgtccatcg acataccgag ttttttcgcc
    24901 gttgcactga taatacgcag gttagcctga tgcggaacca gccagtccag ttgagaacgg
    24961 tcaagattat tcgccgccag cgtctcatca acgatgtgcg ccagttccgt taccgcaacc
    25021 ttgaagactt cgttgcccgc catcgtcaga tgaattgaat tctctggatt cacgcggtcg
    25081 gcgtttggca gcgtcagcaa ttcaccataa ctaccgtcgg catgcagatg ggtggaaatg
    25141 attcccggct cttcagaggc agccagcacc gcagcgcccg cgccatcgcc aaaaataata
    25201 atagtcccac gatctgttgg atcgcaggtg cgcgccagta catcggaacc gacgaccaga
    25261 gcatacttca ccgccccaga tttcacgtat tgatcggcta cgcttaatgc ataggtgaaa
    25321 cctgcgcagg ctgctgcaac gtcaaatgcc gggcaacctt taatgcccaa catgctttga
    25381 atctgacaag ctgcgctcgg gaaagcgtgc gtagcagaag tcgttgccac aacgatcagg
    25441 ccaatctggt ctttctcaat gcccgccatc tcaattgcgc gtgtcgccgc ttcaaagccc
    25501 atggttgaaa cggtttcgtt tggcgcggca atgtggcgtt cgcggatacc ggtacgagtg
    25561 acaatccact cgtcagaggt gtccaccatt ttttccaaat cggcgtttgt ccgcacttgt
    25621 tcgggcagat agctgccagt accaataatc ttcgtataca tgtacgctca gtcacttttc
    25681 ggttatatac cgtcacttgc aaactgcgag ttcgctggca gcgtcctgct accgcagagt
    25741 tccgcttttg ccaccgtcca gcagctcaaa accagctggg tatacagatt ccaggcgagc
    25801 ggcaattcgc tgaggaactt gtcgctgcac cgcctgcact gcctgttcaa tcgcgaccgc
    25861 aaaagctcgc tgattggctg caccatgact ttttatcacc gtgccgcgca atcctaacag
    25921 acaggcgccg ttatactggt cggggttgag gtgactgaat cgcctcgtca ggctcttttg
    25981 tagccaacgc tttaataaca gtagccacca cgaccgtttt ttcccttcac cctgagattt
    26041 cagcagagaa aggaacatcc tgacaacacc ttccatcgtc tttaacgtga catttcctgt
    26101 aaagccgtca caaaccagca catctgtctt gccagttaac aactcattgg cttcaagata
    26161 gccgatataa ttgatagaag ggattgtttt aagcaccgct gaggcatccc gaatactgtc
    26221 aagacccttt acttcttctt caccaatatt gagcaacgcc acgcgaggat tgggaatttc
    26281 caccacctct tcagccagaa ctgagcccat aatggcaaat tgcaccaaca ttgtgctgtc
    26341 acaatcgacg ttggccccta aatcgaggac caccgttttg cccttttgct gatgtggtaa
    26401 taccgtcacc agcgccggac gctcaatccc ctccaggggc ttgagtaata attttgccag
    26461 ccccatcagt gccccggtat taccggcact gacacaggct tgcgctcgac cttctttcac
    26521 cagctccagg gccatacgca ttgaactccc acgactggcg cggatagctt gcgaaggccg
    26581 ggcatcactg gcgataactg actgcgcagg aataatctgc agacgcgaac gttgttcaaa
    26641 gtcagcttta gcaagtaatg gcgtgatggc gtcgggattg ccgactaaaa gaagagtgag
    26701 ttgcgaatta gaattcagtg cctgcaatgc tgcaggcact gtcacggaag ggccaaaatc
    26761 ccctcccatg acatctaacg ccagggttag acgtgtcaag gtatcgtcgc cgcccggttt
    26821 ggtctttccc cagttgcctg gggaaaatcc tcactaagct tcatcacgca gacgcgtgat
    26881 tacttagcga tgaccttgcg gccgcggtag taaccgtcgg cagtgatgtg gtgacgcagg
    26941 tgtttttcac cagaagtttt gtctacagac aggctggtga ctgcggtcag cgcgtcatgg
    27001 gaacgacgca tgccacgttt ggaacgggtt ggtttattct gttgtacggc catggacctt
    27061 actcctcaat tacggtttat ccccgatcca acggggagca ccaattactt acgctttaag
    27121 ctggctaata cggcaaatgg gtttggcttt tgcacttctt caggcagttc accaaagacc
    27181 atatccgctt cggacacttc acagtgttca gaatcatgca ccggaactac cggcaaggcg
    27241 aggatgattt catcttcaac cattgcaagc agatcgattt caccgaattc gttaacctca
    27301 atcggttcat acgcttccgg cagtgcttca gcctgttcgt ctgaacgcac aggactaaaa
    27361 caatacgttg tgtagacctg atgagtaaac ggcttcccgc aacgctgaca ctcgagcgtt
    27421 accgtcacct tcgcatcgcc gtttaacact gcgagacgtt ggttatcgat agcgaacgac
    27481 atggagcatt ccacatcact gtccacactg actacggatt cggcgacgcg ctcaacctga
    27541 tcaggggtat agataccctg gtaatcaagg cgtttttgag ccgtacgaac cggatcgaga
    27601 gtcaggggga attttacctt ttgcataggg cgcgcatatt aactttgtaa cgtcatagag
    27661 tcaaagaaaa aggcagcctg tggttgcctt ttgccaataa ttcgcacaca ttgcgggtta
    27721 ctgctttatt ttcgttcagt gctgataacg aacatcaacc tgcaaaacga agcgcatagt
    27781 ttaaaatggc tatcatcaat acgctatatc tgatggaaaa aaatatgcct aaacttattt
    27841 tagcctccac ctcgccatgg cgtcgcgctc ttctggaaaa actgcaaatc tcttttgaat
    27901 gtgcagcacc tgaggtcgac gaaaccccac gcagcgacga atcaccgcga cagttggtgc
    27961 ttcgactggc acaagaaaaa gcgcaatctc tggcgtcacg ttatccggat catttaatta
    28021 ttggttcaga ccaggtatgt gttcttgatg gcgaaatcac cggtaaaccg ttaacggaag
    28081 aaaatgcccg tctgcaatta cgcaaagcca gcggcaatat cgtcaccttc tatactggac
    28141 tggcgttgtt taattcggcg aatgggcatc tacaaacaga agtggagcct tttgacgtcc
    28201 atttccgtca cctaagcgag gcggagattg ataattacgt gcgtaaggag catcccctgc
    28261 actgcgcggg tagctttaag agtgaaggat ttggcattac gctgtttgag cgtttagagg
    28321 gacgtgaccc taacacgctg gttggtttac cgctaatcgc gctgtgtcag atgttacgtc
    28381 gggaagggaa aaacccgctg atgggatagt tgtttgcgcc ggatgcggtt acgtcgcatc
    28441 cggcaatcgg tgcaggcggc ctgatgcggc gtaaacgcct tatcaggcca accgcccatt
    28501 atcaggctta tatgcttgta ttagcgcgcg ttacgcagtt tttgcaaaca acgcttcaaa
    28561 ccttcatcca tcggcgcttc gatacgcatc acctcaccgg tccccggatg ggtaaacttc
    28621 aacgctgcgg cgtgcaggaa cagacgattt aatcccgtgc ctgcttcagt gagctgtctg
    28681 tcaaattcac ggtcaccgta gcgatcgtca aaggcaatcg gatgacccgc atactgtgta
    28741 tgcacacgga tctgatgagt acgtccggtt accgggctac aacgcaccag ggtggcaaat
    28801 gcatagcgtt cttccacttt aaagcgtgtt tccgacggtt tgccttcctg actgacacgc
    28861 acgatacgtt cgccgctttg cagaatattt ttcaataacg gcgcttgaac gctcttcaca
    28921 tgcgactgcc actgaccgcg caccagcgcc aggtaatctt tttgcattcc tttttcacgt
    28981 aattgctcat gcagagaacg caacgccgag cgttttttcg ctaccagcaa cacacctgaa
    29041 gtgtcccggt cgagacgatg aaccagttca aggaaccgcg cttccgggcg caacgcccgc
    29101 aaaccttcga taacgccgaa gcttaaacca ctgccgccat gtaccgccgt accggaaggt
    29161 ttattcagca ccaggatatg atcatcttca tataagatga cgtccgccag cgccgccacc
    29221 ttttgcagat gtggcgaaac cgcctcttct tcccgctcag caacgcgaac cggtgggata
    29281 cgcacctcat cacccgcttc gagtttatat tcaggcttaa tacgtttttt gttcacccgc
    29341 acttcacctt tacgcaaaat acggtaaatc atactttttg gtacgccttt caattgggta
    29401 cgcaaaaagt tatcgatacg ttgccccgct tcgtcagcgg tgatagcaac aatttttacg
    29461 gatggagtct ctgttttcat ggtgggcgat tctaattagc caacaggatt cgcgccactc
    29521 atttttctat gcttatattt agttttgcac cttattactt cactacgtga tcactttatt
    29581 gatggttatt aaaccaatca ccagcaagaa gtgaaaaaac tgtgagtaag cgggtgataa
    29641 atggtaaaag tcatcttgct ataacaaggc ttgcagtgga ataatgaggc cgtttccgtg
    29701 tccatccttg ttaaaacaag aaattttacg gaataaccca ttttgcccga ccgatcatcc
    29761 acgcagcaat ggcgtaagac gtattgatct ttcaggcagt tagcgggctg cgggttgcag
    29821 tccttaccgg tagatggaaa tatttctgga gagtaatacc cagtctgttt ctttgataat
    29881 tgcgctgttt ttccgcatga aaaacgggca accgacactc tgcgcctctt tgagctgacg
    29941 ataaccgtga ggttggcgac gcgactagac acgaggccat cggttcacac ccggaaaggc
    30001 gttactttgc ccgcagctta gtcgtcaatg taagaataat gagtaagtta cgatgaaaag
    30061 aatgttaatc aacgcaactc agcaggaaga gttgcgcgtt gcccttgtag atgggcagcg
    30121 tctgtatgac ctagatatcg aaagtccagg gcacgagcag aaaaaggcaa acatctacaa
    30181 aggtaaaatc acccgcattg aaccgagtct ggaagctgct tttgttgatt acggcgctga
    30241 acgtcacggt ttcctcccac taaaagaaat tgcccgcgaa tatttccctg ctaactacag
    30301 tgctcatggt cgtcccaaca ttaaagatgt gttgcgtgaa ggtcaggaag tcattgttca
    30361 gatcgataaa gaagagcgcg gcaacaaagg cgcggcatta accaccttta tcagtctggc
    30421 gggtagctat ctggttctga tgccaaataa cccgcgcgca ggtggcattt ctcgccgtat
    30481 cgaaggcgat gaccgtaccg aattaaaaga agcactggca agccttgaac tgccggaagg
    30541 catggggctt atcgtgcgca ccgctggcgt aggcaaatct gctgaggcgc tgcaatggga
    30601 tttaagcttc cgcctgaaac actgggaagc catcaaaaaa gccgctgaaa gccgcccggc
    30661 tccgttcctg attcatcagg agagcaacgt aatcgttcgc gccttccgcg attatttacg
    30721 tcaggatatc ggcgaaatcc ttatcgataa cccgaaagtc ctcgaactgg cacgtcagca
    30781 tatcgctgca ttaggtcgcc cggatttcag cagcaaaatc aaactgtaca ccggcgagat
    30841 cccgctgttc agccactacc agatcgagtc gcagatcgag tccgccttcc agcgtgaagt
    30901 ccgtctgcca tccggtggtt ccattgttat cgacagcacc gaagcgttaa cagccatcga
    30961 catcaactcc gcacgcgcga cccgcggcgg cgatatcgaa gaaactgcgt ttaacaccaa
    31021 cctcgaagcc gccgatgaga ttgctcgtca gctgcgcctg cgtgacctcg gcggcctgat
    31081 tgttatcgac ttcatcgaca tgacgccagt acgccaccag cgtgcggtag aaaaccgtct
    31141 gcgtgaagcg gtgcgtcagg accgtgcgcg tattcaaatc agccatattt ctcgctttgg
    31201 cctgctggaa atgtcccgcc agcgcctgag cccatcactg ggtgaatcca gccatcacgt
    31261 ctgcccgcgc tgctccggta ccggtaccgt gcgtgacaac gaatcgctgt cgctctctat
    31321 tctgcgtttg atcgaagaag aagcgctgaa agagaacacc caggaagttc acgccattgt
    31381 tcctgtgcca atcgcttctt atctgctgaa tgaaaaacgt tctgcggtga atgcgattga
    31441 aacgcgtcag gacggtgttc gctgcgtgat tgtgccaaac gatcagatgg aaaccccgca
    31501 ctaccacgtg ttgcgcgtgc gcaaagggga agaaacgccg accttaagct acatgctgcc
    31561 gaagctgcat gaagaagcga tggcgctgcc gtctgaagaa gagttcgctg aacgtaagcg
    31621 tcctgaacaa cctgcactgg caacctttgc catgccggat gtgccgccag cgccaacccc
    31681 agctgaacct gccgcgactg tcgtagcccc agcaccgaaa gctgcaacgg caacaccagc
    31741 agctcctgca caacctgggc tgttaagccg cttcttcggc gcactgaaag cgctgttcag
    31801 cggtggtgaa gaagccaaac cgaccgagca accaacaccg aaagcagaag cgaaaccgga
    31861 acgtcaacag gatcgtcgca aacctcgtca gagcaaccgc cgtgaccgta atgagcgccg
    31921 cgacacccgt agtgaacgta ctgaaggcag cgataatcgc gaagaaaacc gtcgtaatcg
    31981 tcgccaggca cagcagcaaa ctgccgagac gcgtgagagc cgtcagcagg ctgaggtaac
    32041 ggaaaaagca cgtaccaccg acgagcagca agcgccgcgt cgtgaacgta gccgccgccg
    32101 taacgatgat aaacgtcagg cgcaacaaga agcgaaggcg ctgaatgttg aagagcaatc
    32161 tgttcaggaa accgaacagg aagaacgtgt acgtccggtt cagccgcgtc gtaaacagcg
    32221 tcagctcaat cagaaagtgc gttacgagca aagcgtagcc gaagaagcgg tagtcgcacc
    32281 ggtggttgaa gaaactgccg ctgccgaacc aattgttcag gaagctccag ctccacgcac
    32341 agaactggtg aaagtcccgc tgccagtcgt agcgcaaact gcaccagaac agcaagaaga
    32401 gaacaatgct gataaccgtg acaacggtgg catgccgcgt cgttctcgcc gctcgcctcg
    32461 tcacctgcgc gtgagtggtc agcgtcgtcg tcgctatcgt gacgagcgtt atccaaccca
    32521 gtcgccaatg ccgttgaccg tagcgtgcgc gtctccggaa ctggcctctg gcaaagtctg
    32581 gatccgctat ccaattgtac gtccgcaaga tgtacaagtt gaagagcagc gcgaacagga
    32641 agaagtacaa gtgcagccga tggtgactga ggtccctgtc gccgccgctg tcgaacctgt
    32701 tgttagcgcg ccagttgttg aagaaatggc tgaagtcgta gaagcccccg taccggttgc
    32761 cgaaccgcaa ccggaagtgg ttgaaacgac gcatcctgaa gtgattgccg ccgcggtaac
    32821 tgaacagccg caggtgatta ccgagtctga tgttgccgta gcccaggaag tcgcagaaca
    32881 cgcagaaccg gtggttgaac cgcaggaaga gacggcagat attgaagaag ttgccgaaac
    32941 tgctgaggtt gtggttgctg aacctgaagt tgttgctcaa cctgccgcgc cagtcgtcgc
    33001 tgaagtcgca gcagaagttg aaacggtagc cgcggttgaa ccagagatca ccgttgagca
    33061 taaccacgct accgcgccaa tgacgcgcgc tccggcaccg gaatatgttc cggaggcacc
    33121 gcgtcacagt gactggcagc gccctacttt tgccttcgaa ggtaaaggtg ccgcaggtgg
    33181 tcatacggca acgcatcatg cctctgccgc tcctgcgcgt ccgcaacctg ttgagtaata
    33241 attagctcaa agtaatcaag ccctggtaac tgccagggct tttttatttc atgtttgaat
    33301 caacatatta aaattcatca aatggcatcc ttgctaacca acaatgcaaa ataggcaaaa
    33361 aaaatgcccg ccccggcgat cccagagcgg gcagacaaaa catacccagt ttcatgatat
    33421 gttttaaagc gagcttattt gttgagctgg aagagcgaca atccctgcat atcggtaaat
    33481 gctttatacg atgcctgcaa tgccgtttgc tgcatgatgt aagatgaaat agttgcattc
    33541 cagtccacat caaccagatc gctcatctgc tgcgtttgcc ctaaagcgcg atcgctacct
    33601 aaggaatcca gcgactccag tttgttcagc tgcgtgccta attccgtgcg aacggtaagg
    33661 acattattca gcgagttttt cagtccacgg ttggttttat ccaatgccgc agcggcgatt
    33721 tctttatccg cttcgctatc cgcgaccggc gttttcaggg ctgcgatggc gctatccagc
    33781 atggcaaaaa gattggtttc agaagcgcta ccgtctggtt ccgctaccgc gttactggta
    33841 atactgtcga aaattttgtc gcccgtatgt cctatcacca tcgaacgcga agcatcgacc
    33901 tgttgcttaa tactttctgt tccgccgaca taatcaccgt tagcctcact aaacggcgca
    33961 gtctctgttt tataaccggc aaaaatgtag cgcccattac cgtcagtggt gttcgccaga
    34021 ttcagcaact ggtcacgaag cccctgaata tccgtagcca gcgaggcccg gtcatcgtca
    34081 ctcaaggtgc cattgctggc gtagacaatt ttttcctgag cattctggat agcagtggtg
    34141 acctggctaa gtacactctc ttccagtgac actttttgag tggcgaaagt acgtgccagc
    34201 gtgtactggc tgttttgcgc ctgtgcctgg gagagaacaa cagcttgtga tgcagcaatg
    34261 ggatcgtctg aagggttaac gactcgctta cccgtcgaca tctgctcgcc gtacttcatc
    34321 cattctgcct gagaattggt gatgccgcgc atgttttgct ggtacatcat ctgggtactg
    34381 aaacgcattt tatctcccct tagcgaatgt taatcagcgc atcaaaaatc gcgtttgccg
    34441 tctgcagaac ctgcgcattc gccaggtaat actgctgaaa acgttgcaga tttccgtatt
    34501 cctcatcgag attgacaccg gaaatcgact gctgctgatt ggaaagctgc gtcaccacat
    34561 taccttgcgt ggtgctactg gttttcaacg tcgcggtttt attaccgata tcactcacta
    34621 acgaggcata agcgtcgtta aaggatttcg caccgcccac cgttttactg ttgctttgca
    34681 gatccagcag ggcctgaccg ttgcggttgt cgctatcacc cgcatcttct tcgctcgcca
    34741 tcgcgatttt cgcttcgtcg gtgattaata catccatgtt gacgatggcg tcacttactg
    34801 gtttcagcgt gaagctgtcg ttaacggcag gcgttcctgt aaacgtcaac tccagaccat
    34861 caaatgccac tttaccgttg gcatctggtg tcgccgtaaa agtggtattg ctggcaaggc
    34921 gggtgacctg ccactgatta ttatcgaacg agattttgta atctgtcgcc agtaccacgg
    34981 aggcatcagt taccgtggca ccgatcgcaa cgtcaccttt gtttttcgtg ttttgctgaa
    35041 ccgcgggatt accgatagta aagaaatctt caccagcatc gccgttagca tcaaatccgg
    35101 ctttgtgttg gctgttgaaa gcctcggcaa atgccagcgc cagttgtcca agcgtattac
    35161 gcgtctggtc cagatcctga gaacggaatg tcagaatgcc gcccagcgac ccggtattca
    35221 gtaatttctc cgggatctca atattgcctg ccgtcccatc aacgtaagcg acagtcgtac
    35281 gagaagggtc ggcgctggaa ggaactgccg ccagttgccg cgccgtactt ccctgaacca
    35341 gtgagtaacc attggcatcg tgatgttata agtaccgcca tcctgaacgc tgacttctac
    35401 gccaacaatc tggtttaatt cgctcaccag ttgatcgcgt tgatccagca gattgttagg
    35461 tgacgcccct gcccccacgc ctgtcaggcg cgagatttga tcgttcaggc tggcaatttg
    35521 tttagcgtaa ttgttgatct gatcaacgct ggcacctatc gcgatattga cctgtttgtc
    35581 ctggtcgcgc agatattgat cggtggtttt aaactgattc accaaccctt ctgatttccc
    35641 aatcagcgcc tggcgcgccg ccgggtcttc cgcgttactc accagcgttt gcaggctggt
    35701 gaagaaatcc tgcatctgtg ttgccagcga agaggtactg gtggagagca tattgtcgat
    35761 tttcgacatc tgctcatagc gggcggtcag gccgctactt tgcgtctgca cagcacgtaa
    35821 ctggttggta ataaacgtat catactcacg ctgcacacca gaaacgtaga cgccattgcc
    35881 aacccagccg ccagcgccca acgtgctatt ggcctgcgcc ataatagtgg tttggcgggt
    35941 atatccggca acgttatagc tggagatatt attacttgcc gtatttaacg ccgcctgggc
    36001 cgcgttcagt ccgctcatgg cgttattgat caagctggac atggaggttc cttataagcc
    36061 ttcaattaca gagtattatc ggcagcgacc cgccggactt gagttattca gaacagatta
    36121 tcaatgttca tgctgtaggt tttgctcacc tggtcgctta tcgatttcat ctgctggatc
    36181 atgttggtga gtttacgggc atagtgagga tcggtagcat agcccgcgtc ctgtaggacc
    36241 tgcgccccct gttccgcact cgcggcggtc gtcacggcgg cgtagcgcgg gttacgtgtt
    36301 aacagcccaa cgtaatccga caatgcttcc agatacgagc tgtagacccg aaacttcgct
    36361 tttactttct tcgcttcgcc gttttcatat tcagtcgtgg tgatttcagt gacttgccct
    36421 ttccagttgc cagaggcttt gacaccaaac aggttatagc ttggctcgcc gttttcgcgg
    36481 cggatttgcc gttgccccca accagattca agcgcagcct gagcgaggat caaatgatgt
    36541 ggcacaccgc tttgctggct tgccagttgg gcgggcagcg agagttgcgc gaggaatgct
    36601 ctactgtcac ccggcagcga atcatcgtag ttacgtggta cggccttttg caccagctgc
    36661 gaaagcgtct ggttttgata acgcaccacg gtttcgagcg ggaatttcat cggtgctgct
    36721 ggcgtggact cctctggcaa tggttgttct ggcgtcatct gtttaaccat catctctgca
    36781 agccccagac ctttgcccgc cgtcatctgt tgggcaattt gctggtcata catactggta
    36841 tacagacgag tgtgctcgct gctgaacagg ccatcttttg gtaaagcgtc gcgcatgctt
    36901 ttcaacatca tctgcacgaa catcccttcc acctgacggg ccaccggacg gatatttgcc
    36961 gccggatctt cgctcgcttt cgcctttagt tcgttgagtg attgcgcatc ccaggccgca
    37021 cttgccagta gtttgctgtc gctgatcatc agatgatttc cagttttgcc cgcagacatc
    37081 ccgcactttg cattgattgc aaaatagaca tcagatccat cggcgtagcg cccagcgcat
    37141 tgagcgcacg caccacgtta ttgaggctgg cgctggaacg tacgctttgc agcgaaccgc
    37201 cgctctggcg taaatcgatc tgcgtttgtg gagtaacaac agtctgtcca ccaccaaacg
    37261 gtgtatctgg ctggctgaca ttggcctgac gattaactgt tactgagaga tttccctgcg
    37321 ctaccgcgca gctgtcgagg gtcacttctc gattcatcac caccgaaccg gtgcgcgagt
    37381 taatcactac tttagcgtcc tgcggggtga cattaacctg catattctgg atatcggcaa
    37441 ggaaacggac ctgggaactg ttgccacttg gtacgcgcac ctgaatagtc cgcgcatcta
    37501 acgcggtggc gctgccatat ccacgcacgc ggttgatggt gtcagcgatt tgctgcgcca
    37561 tgctgaaatc ttcgtcgtta agttgcaaat taagggtatt cccgacgcca aactggctgg
    37621 gcaattcacg ttcaataacc gcaccattgg tgatccgtcc accgttcagt tggttaacct
    37681 gaacactgct accgcccgcg gaggctcctg cgccgccaac cagaatattg ccctgcgcca
    37741 gcgcatacac ctgactgtca acgcccttaa gcggtgtcat caacaacgta cctccacgca
    37801 agcttttggc attacccatg gaagaaacca ccacatcgat ggtttgcccc tgacgtccaa
    37861 acggaggaag tgacactgtc accattacca cagcgacgtt ttttagctgc atattggtgc
    37921 ccgtcggaac ggtaattccc agctgtgaga gcatgttatt aagcgtttgt gtggtaaacg
    37981 gcgtctgggt tgtctggtca ccagtgccat ccagccccac taccaggcca tagccaatca
    38041 gtgagttttg ccttaccccc tgaacactgg tgagatcgcg aatacgctca gcctgagccg
    38101 ccgtcgtaac cagtagaaga attaatgcag agagaaattt aatcaccaca gcctcactta
    38161 cattggcgac aggttaagga agaaacgctg caaccagccc atattttgcg cttcgttaat
    38221 gtagccattg cctacgtatt caatacgcgc atccgccacc tgagtagacg gtacggtatt
    38281 gctgccgctg atagtgcgtg gattaaccac gcccgagaag cgaataaatt cggtaccctg
    38341 attaatggca atctgttttt cacccaccac atgcaggttg ccgttgacca gtacctggtc
    38401 aaccgtcacc gtcaacgtgc cgctaaaggt attgctggca ttggccccgc cctttccgtt
    38461 gaacgtgtta ccaccggagg cttcgacatc ggcacgagcg ttaccaaaca gcccctgcaa
    38521 atagcgcggc acagtatcaa agccaaaatt agttttaccg tcacggctgg cattcgcaga
    38581 ggagcttttg ctggcgctga cgttctcctg caacacgatg gtcagcgtat cgccaatatt
    38641 gcgtggtcga cgatcttcaa acagcggttg atagccatag ttaatcggct gagcagactg
    38701 gaaaatagaa ccgttggcga cgggcgtcgg accgggaacc ggttgtgcac tggtcgcccc
    38761 ctgcaccagc ggcgtggagg gtatccaggc gcagccggtt agtgaaagca ccaacaagct
    38821 ggaaatggca taagtatgcg cagcgttttt ttgcatggct atcatcttca aaaatcagta
    38881 aaccggtgaa cctgccaccg gttatgcctt agagttgcgt cagtttttgc agcatctgat
    38941 cggtggtgga caccgcttta ctgttgattt cgtaagcgcg ttgcacctga atcatattga
    39001 ccagttcttc cgccacgttg acgttagacg tttcaacata cccttgatac agcagtcccg
    39061 cgccgttcag gcccggcgtg ctttcgttcg gtgcaccgga ggattgcgtt tcggtgtaga
    39121 ggttttcgcc aatgctctcc agtccggtat cattcataaa ggtggtgaga ttgagctgcc
    39181 caacctgaac cggagctgcc tggccttgtt gggttacgct gaccacgcca tcacgaccga
    39241 tggtgatact taacgcattc gccggaatgg tgatcgctgg ctgcacctga aaaccaccag
    39301 ccgtcaccag ctgcccgttc tgatccacct ggaaagagcc gtcacgggta taggctgatg
    39361 agccatctgg caacattacc tggaaaaagc cctgcccttt aatcgcgaca tctttgctgt
    39421 tattggtctg cgacagattg ccctggctgt gtaagcgttc agtggcgacc gggcgtaccc
    39481 ccgtgccgat ttgtaatccg gagggtaagg tggtttgttc ggaagactgt gcccccggct
    39541 ggcgaatggt ttgataaagc agatcttcaa acaccgcgcg ctgacgctta aaaccgttag
    39601 tactgacgtt tgccaggttg ttggcaatga cgtccatatt ggtttgctgg gcgtcaaggc
    39661 ccgttttggc gatccataat gaactgatca tataatgtcc tgtagtggat tgctgacaag
    39721 cagagcagaa gcacgcgaca gtgtgaattt cccctcaccc taaccctctc cccaaagggg
    39781 cgagggaata gtccgcgcgc ttttattgca atgtcatcaa tcgttcaatt aactcatcga
    39841 cagcagttgg ttggcacggc ctgcgttatc atcgacgctg ctgatcacct tcatctgcat
    39901 ttcaaaacgc cgcgcgctgg caatcatgtc gctcattgcc gcaacggcat tgacgttact
    39961 gccttccaga acccccgaca tcacacgcaa ggttggatct gcctgcagta ccggccctcg
    40021 cgtggcctgg gtttctgcgc ttaaacgaaa aatgccgtcg tcaccgcgct gcacttcgct
    40081 gcccgtggct ttcaccagtt tcagacgccc tactggcgca actgtatttg ccggatcgcc
    40141 cggattgagc gccgagattg tgccatcggc agcaatcgtg atttccgccc cttccggcac
    40201 agcaattggc cccgcctcgc ctatcaccgg atgcccctga attgtcagtt gcccggtggg
    40261 atcaacctga atgctgccat tacgcgtata cccttcgctg ccgtcagcgg tctgcacggc
    40321 cagccatcct gctgcaacgc aacgtccagc gggcgcgagg tgtaatccat cttgccgggc
    40381 gtcatgtctg cacccggtgt tgacgccgtt accagcgtgc gcgtgggcag tgaaagccct
    40441 tccaccggca ctgcgcgcaa ggcgtttaac tgcgcgcgaa aaccgggcgt agaggcattg
    40501 gccagattac tggcggttac cgcctgttga ttcagcgtct ggctggctgc tcccatcgca
    40561 gtataaattg cgtgatccat tgagctatcc cgtcagcgat tagcgtaagt taacccgcgt
    40621 gttgaggatc tggtcctggg ttttgatggt ctgggcgtta gactgatagt tacgctgggc
    40681 aacgatcata ttgaccagtt ctttactgag atcgacgttg gacgcttcca gcgcaccgtt
    40741 ggtcagggtg ccaaagtttc ccgtcccggc tgtccccaac agcgccacgc cagaagattg
    40801 cgtcgcagac cagacgttgt cgccttcgga tgccagacct tcgttgttgg caaagttcgc
    40861 cagtacaatc tgccccagca gttgggtttg ttcgttggaa tagttgccga caaccgtacc
    40921 gtcatcattg atttgataac tcaccagatc gcccggtttg tagccattct gggtggttgc
    40981 cacaatgttg ttagcgccgg tattttgctg catggagttg aggaagctca gactaaacgt
    41041 ggcgggttct gcaccgttaa ttgcgccggt tgcgatatta ttcgccatcg cacatgtgcc
    41101 attagcatta aattccagtg ttgtcgctgt cttcgcaatg ctgtttggat cactgctatc
    41161 ctgggtgtag acctgccagt tattatcccc ggtcttcaca aaatagacgc tcatgtcatg
    41221 agcattaccc tgactgtcga aaacagtcac cgaacctttt ttgttatagc tatccgcatt
    41281 gctggcgctg aatggcgtaa cagtaggaag cggatcactg gaattcaggt tgatctgcat
    41341 cgacgccgtg gtggtagttt tcgctgccat cagggtattc gggatcgaaa tattggtcgg
    41401 attcgcccct tgctgaatag tcggcggcgt accggttgcc gggtaacccg tcagctgtaa
    41461 accttgcata ttcaacaggt tacggttttc atccagctta aattgtccgt tacggctgta
    41521 gaacaccgaa ccgttgctgt ctaccagacg gaaaaaaccg ttctggctga tagcaacgtc
    41581 cagacctcgc ccggtgttgg tggtcgtgcc atcggtaaag tcctgagtga taccggcaac
    41641 ttttaccccc agtcccactt tcgaaccggc aaacatatcg gcaaaagagg ccgtgcctga
    41701 tttaaagccg taggtggcgg agttggcgat attgttgcca ataacatcga ggttggtggc
    41761 ggcagcgttt aatccgctaa ccgcttgaga aaaggccatg actgactcct gaaatgtgaa
    41821 ggcttaaatt atctgccgta cttcgtcgag ggtggtggtg ccgtaagtgc cgagatccag
    41881 cgtattaccg ctgttgccgc ggatcacacc ctgcaccaga gcaaactgca gcggctgggc
    41941 aaccagttgt gtaccaccgt tactggcgct aatcgctaca ttgtaagaac cgttcggcgc
    42001 agtgctgcca tcagtcaacg taccgtccca ggtgaaactg tgaactccgg cggtcagttc
    42061 accaatatca atggtgcgca caaccgcgcc atttttatcg gtgatggtgg ccgtaacttt
    42121 gtctgccgcc tgttgcagct caacaccaaa cggcgtggtc gtggtcacag ccccttcttc
    42181 actgccggtt cccgcaagaa cagttgtgcc ggggatcatc acgccgtgac cgatcaggtt
    42241 actggcctgt aacgactggc tgttatcaat ctgtccggaa atagatccga gcgtggtatt
    42301 gagtttttca atcccgctga ccgtgctgat ttgtgccaat tgcgacgtca gctcgttgtt
    42361 ttccattgga ttggtcgggt cctagttttt cagctgcgcc accagcaaag tcagaaaact
    42421 gctttgtaaa tctgcggcgt tgctgcccgt gagcgaacta ctgctggtgg tactgacgcc
    42481 ggtatttgtc ggatcggtgg tggttaccgc aatggacata gctttctcct ttattgaccg
    42541 agcgtaaggg ttttcagcat catgcttttc accgtgttga gcacttcaac attggcctga
    42601 tagctgcgtg acgccgacat ggtgttaacc atctccccga cgacatcaac gttcggcatt
    42661 tttacgtagc cctttgcatc tgccagcgga ttacctggtt cataaaccag tttgtccggg
    42721 gcctgacttt cttaacatcg gcaaccttta cgccgcctgt cgcagcacct ggtgcagcgt
    42781 taacctggaa taccacctgt tttgcccgat atggctgtcc atcaggaccg gtcacgctat
    42841 cagcatttgc cagattactg gccgccacgt tcaggcgctg ggactgggca gttaacgccg
    42901 acccggcgat atcaaaaata ttcagcagtg ccatccgtta atttccgctc tgtaaaacgt
    42961 tcatcatgcc tttgatttgc ccgctcaacg cgctaaggct catctggtat tgcaggctgt
    43021 tatcggcaaa ctgggtgact tcctgacgct gggcgcgcag attgagcgcc tcttgttgaa
    43081 aacgtaaggc ggcgtcgagc ttatcgagca tatctcctcc gcaggtatca aaattctgcc
    43141 atcagcttaa atgcctttac gcgcgcgtta tcggcggaat aaacgcaaaa tgggtcgcta
    43201 tttatgccgt tgatggtcat tgcggacagg tacaactcac gttgtagaaa tggctggggg
    43261 cgaaaatgct gacaataaaa cgtagcgtgg cgatcatcgc gatactgttc agtccgttaa
    43321 gtacggcgag caatctcaca tcgcaattgc acaacttttt tagcgcccaa ctcgcggggg
    43381 taagtgatga ggttcgtgtt tctattcgta cagcgcccaa tctactaccg ccatgcgagc
    43441 agccattgct ttcgatgagc aataattccc gcctgtgggg caatgtgaat gtgttggcac
    43501 gctgcggtaa cgacaaacga tatttacagg ttaatgtgca ggccacagga aattatgtgg
    43561 ttgccgcgat gcctattgtg cggggaggaa agctggaagc tggcaatgtc aaactgaaac
    43621 gtggacgcct ggataccctg ccaccgcgta cggtgctgga tatcaatcaa cttgttgatg
    43681 ccgttagcct gcgcgatcta tcacccgatc aacctatcca gttaacccag tttcgccagg
    43741 catggcgggt aaaagcggga caacgcgtca atgtgatcgc cagcggtgat gggtttagcg
    43801 ccaacgcaga aggtcaggcg ctgaacaatg cagccgtcgc acagaatgcg cgggtgcgca
    43861 tggtatcggg acaggtagtc agcggcgttg ttgatgcaga tgggaatatt cttataaacc
    43921 tgtaagctgt taaagattac ccgtcccttg ccgataaata agcaacacat gataaaagcg
    43981 ccctcaatga ggaataaacc atgagtattg atcgcacttc gcctctgaag cctgtaagca
    44041 ccgttcaacc gcgcgaaacc actgacgcgc cggtaacgaa cagccgcctc caccagcacc
    44101 agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac ccggcagcag tgatatcaat
    44161 cttgaacgtg tcgaagcgtt aaaactggcg attcgtaacg gtgaactaaa aatggacacc
    44221 ggcaaaattg ccgatgcgct gatcaacgaa gcgcagcaag acttgcagag taactgatcg
    44281 tatgacacgt cttgcagaga tcctcgacca gatgtccgct gtgcttaacg atctcaaaac
    44341 ggtaatggat catgagcagc aacatctctc tatggggcag atcaacggca gccagttgca
    44401 atggattaca gaacaaaaaa gctcactgct ggcgacgctg gattacctcg aacagttacg
    44461 caagaaagaa cccaatacag caaatagcgt tgatattagt caacgctggc aggaaattac
    44521 tgtgaaaacg cagcaactac gccaaatgaa tcaacataac ggctggttac tggaaggaca
    44581 gattgagcgc aatcaacagg cgctggaaat gttgaaaccg catcaggaac cgacgctata
    44641 tggggcgaac ggtcagacct caacacccca tcgcggcagt aaaaagattt cgatctgaat
    44701 attatctccg gcctgcactg caggccggaa tgcattgtta caccgtccgg cgggcaaatt
    44761 ctttaacttt gaagcccagt accgccagtg cagcgaagta cgcggcaatc cccgccagca
    44821 cgaccgccat taaacgcagt aagcgccagg gcatggtacc caatgaccac tccggcatga
    44881 tatgtaacat acctaaaagc acgccagaca tcaccagtac cgccaccacc agacgcaaca
    44941 gaaacgccat ccagccgggt tgcggagtaa agattttctg cttacgcaac tgccagtaaa
    45001 gcagcgaagc attcagacac gccgccagac caatagaaag tgacagcccg gcatgtttca
    45061 acggaccaat aaacgccagg ttcatcaatt gcgttaaaat cagcgtaacg atggcaattt
    45121 tcactggcgt tttaatgtct tggcgggaat aaaagccagg agccaacact ttcactacaa
    45181 tcaggccaat caaacccacc gagtaggcaa ttaacgcccg ctgggtcatc agcgcatcaa
    45241 acgcggtaaa tttaccgtac tggaacagcg aaacggttaa cggacctgaa agaatgccca
    45301 acgcaaccgc actcggcagt gccaacagga aacaaagacg caacccccag tccatcaaac
    45361 ggttgtattc atcatgattg ccactggcaa aacttttcga cagcgacggc agcaaaatgg
    45421 tgccaagcgc cacgcccagc acaccggagg gaaactccat taagcggtcg gcgtaataca
    45481 tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat ggtgttgatg attaaggaga
    45541 tctggctcac agagacgcca aggatcgccg gtcccatctg tttcaccacg cgcattgctc
    45601 cagcatcgtg gaagttaatg cgcggcagga ccagcatgcc gatcttcttc aggtgcggta
    45661 gctgatacac cagctgcagg atgccgccga ccgtaacagc ccacgccagc gccagcaccg
    45721 gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat catgctgatg ttaagcagtg
    45781 ttggcgcaaa cgccggaatc gagaagcggt tccacgtatt cagaatcgct cccaccagcg
    45841 acgccaggga gatcagcaag atatagggaa aggtaatctt tagtaggtgg ctggtcaggg
    45901 caaatttgtc agctgtgtca gcgaagcctg gcgcggtcac catgatcacc cacggtgcgg
    45961 cgagcatgcc agcgaccgtc acaaccgcca gcgcaagtgt cagcaggcca gaaacataag
    46021 agacaaagac ccgcgtggcg tcttcaccct gcttactttt atattccgcc agaatcggta
    46081 caaatgcctg ggaaaatgcc ccttcggcaa agatacggcg taacaagtta ggaagtttaa
    46141 aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa gattctggcg acaattgcgt
    46201 ctcgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga gctgacggcg gccagcgatt
    46261 ttaataaatt catcggtgtt ctaatccgga cccacgggca aaacgcctgc aaggcaggcg
    46321 ctgaaagagg cgaatagtct acctagatta tggtgaattg ctaccgccag atgttacagg
    46381 gtttattcac tcatcgcatc gcgccagatc ttgtcaacga tacgttgcgc cagcactgcc
    46441 tattcgccgg cggtttgcgg aactgtctga ttttgcacgc attcaatgaa gtgccgcgca
    46501 cagccgacaa atccacgttg ctcaagagtg ctctgccaac caggaatcgg tttattcact
    46561 acaccctgcc cgcgctcctc acgccattcg cgcatatccg taatgtcgat gagcgcaccg
    46621 tcagtcacgg cctgcacggt ttcacgctga cttccggcac ggcgatgcat acaagtggtg
    46681 atctgcaaag gaccggccga aaaatggtgc tcggcaaaca gcatttcgcc agcgtcgtta
    46741 gtcagtagcg taccgccatc cagagaggct ttgccgcccg acaaccacag cgcggtatcc
    46801 accacatgca gataatcatc cagcaacgtg aaataaagat cgtgtggccc gacgctattg
    46861 ctacgatgtt tatccattct tagcgaggct gcggtggcga gttgcgtttt taactcaccg
    46921 tagagtggtg cgaaacgacg gttaaaaccg accatcaacg tcagtttttt ccgcgccgcc
    46981 agttccacca gcctttcagc atcgcgcaga ttttctgcca gcggtttatc gacacagaca
    47041 tgtacccccg cattgagtaa cgtactgacc acgtcaaagt ggctggcggt gctggagtgc
    47101 acaaaaaccg catcacaact ggcggcaagg ctggataacg aatcggcata aggaatgcgc
    47161 cagctgtcac aaattggcag ggctttggcg cgcgtaggcg accaggctcc ttgtaacgtc
    47221 cagtcagacg ctgccgccag caccggtaac cacgcttttt gcgcaatgcc gcctaatccc
    47281 actacgccga tacgtaattt tttcacttta atctcccaga tgggccagca acgaatcaag
    47341 acgttgtttc agttctgcca cttcgatttc cagggcttcg acgcgggtct gtaaatcacc
    47401 gtcaaccatg ttcgacatag ccgttaccgc cggctgatct tcaacctcac cactgaaaag
    47461 atgcatgtag cggctttcgc gtttacccgg ttcgcgggcc agacgcacca caaaaggacc
    47521 atcttcgcga ttcgccagtt gttccagcgt cgactccact tccgccatat cgctgaattc
    47581 atacattcgc gcggcgcggc tgcgcagttc acccagcgtc tgggcaccac gcaataacaa
    47641 cgtggtgatt aacgccactt ctgcggcgct cagtttcaga tcgccaaatt ctgaattaca
    47701 aaaacgttgc tcatatttgg tgacccgatt accaaaaccg ctcactgtgc gtagataatg
    47761 acgtttgacc agattatcca gctgttcctg cacttcggat tcactcagat tcatgaccgg
    47821 ttcacggttc gttttctgat tacaggccgt gactacacca ttgactgaga gcgggtattg
    47881 ctccggcgtg gtcacctgct tttccagcaa gcaaccaatt acgcgagctt ccagggcagt
    47941 aagttgatat ttcatgagat tctccttagc ggccgggcgt ccagtctggg gtagttaatg
    48001 ccgtcagtac gtgatcgcgc cattgtccat caatcaacag atagtctttc gcatagcctt
    48061 ctttttcaaa acccagtcgc gccagtaaat caccgctgcg tttattgtgc ggcatataat
    48121 tagccataat gcgatgaata tgttgggtgc gctgcatata acgaatggct gcggtcaggg
    48181 cttcaaacat gagtcctttg ccctgccatt tttgcccaat cgaataaccg agatagcagg
    48241 catgaaaaga gccgcgaaca acattggaaa aattggcaac gccaataatc tctttttcgt
    48301 ccgggtcgaa taagccaaag tagaaagctg aaccttgttt atgaaattcg ttaatcatcc
    48361 ctagcctggc ctgccagcct gatggataac agtggctttc gtcgcgcact ggctcccagg
    48421 gcttgaggaa atggcgattc tctgcgtaat aatccgccag acgccaggca tcacgatcat
    48481 gcaccagacg cacgaccagt cggtctgtgg ttaagcgcac ttttggcacg ttactgcgat
    48541 agccaaacat tctataccta ctccttcccg taacgtcttt aatacgctgt aagtattact
    48601 atacctgcgc aaccagaccc tgtgaaaagt ccaaaatgcc attttctgca ttattcacat
    48661 atttgataaa aactctctat caaagcgacg ttttgataaa aaaatattgt ccccgaagtg
    48721 atgggaaaag tcttagcggc aatgcagaat agaaagtgct catcttttat ttcccgggag
    48781 gggaaatgtc ccgcgtatcg caggcgagga acctgggtaa atatttcctg ctcatcgata
    48841 atatgctggt cgtgctgggg ttctttgttg tcttcccgct gatctctatc cgcttcgttg
    48901 atcaaatggg ctgggccgcc gtcatggtcg gtattgctct cggtctacgc cagtttattc
    48961 agcaaggtct gggtattttc ggcggcgcaa ttgccgaccg ctttggtgcc aaaccgatga
    49021 ttgttaccgg tatgctgatg cgcgccgccg gattcgccac aatgggtatc gcccacgaac
    49081 cgtggctatt gtggttttca tgcctgctct cgggactcgg cggcacgttg tttgatccgc
    49141 cgcgttcggc gttggtggtg aaactaatcc gtccacaaca acgtggtcgt tttttctcgc
    49201 tgttgatgat gcaggacagt gccagtgcgg tcattggcgc attgttgggg agctggctgt
    49261 tgcaatacga ctttcgcctg gtctgcgcca caggggccgt tttgtttgtg ctatgtgcgg
    49321 cgttcaatgc gtggttgtta ccggcatgga aactctccac cgtacgcacg cccgttcgcg
    49381 aaggcatgac ccgcgtgatg cgtgacaagc gttttgtcac ctatgtcctg acgctggcgg
    49441 gttactacat gctggctgta caagtgatgc tgatgctgcc aattatggtc aacgacgtgg
    49501 ctggcgcgcc ctctgccgtt aaatggatgt atgccattga agcgtgtctg tcgttaacgt
    49561 tgctctaccc tatcgcccgc tggagtgaaa agcattttcg tctggaatac cggttgatgg
    49621 ctgggctgtt gataatgtca ttaagcatga tgccggtggg catggtcagc ggcctacaac
    49681 aacttttcac cctgatttgt ctgttttata tcgggtcgat cattgccgag cctgcgcgtg
    49741 aaaccttaag tgcttcgctg gcggacgcaa gagctcgcgg cagctatatg gggtttagcc
    49801 gtctgggtct ggcgattggc ggcgctattg gttatatcgg tggtggctgg ctgtttgacc
    49861 tgggcaaatc gacgcaccag ccagagcttc cgtggatgat gctgggaatt attggcatct
    49921 tcactttcct tgcgctgggt tggcagttta gtcagaaacg cgccgcgcgt cgtttgctgg
    49981 aacgcgacgc ctgatttgat acacccccct gactcctcca tactgaaagt gaactgacag
    50041 ccaaatggag gagtcacgtg aagctataca tttacgatca ctgcccttac tgcctcaaag
    50101 cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt acatgttctg ctcaacgacg
    50161 acgcagaaac acccacccgg atggtcggtc aaaaacaggt tcccattctg caaaaagatg
    50221 acagccgcta tatgccagaa agcatggata tcgttcacta tgtcgataaa ctcgacggca
    50281 aaccgttact gaccggcaaa cgttcccctg ccattgaaga gtggctgcgc aaggtcaatg
    50341 gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc ggcatttgat gagttttcta
    50401 ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc cagcgcgggt aattttgccg
    50461 acctgctggc ccactctgac ggtctgatta agaatatcag cgatgatttg cgtgcgctgg
    50521 acaaactgat cgtcaaaccg aacgccgtga atggcgaact ttcggaagat gatattcagc
    50581 tatttccgct actgcgtaat ctgacgctgg tagccggaat caactggcca agccgcgttg
    50641 ctgattacca cgataatatg gcgaaacaga cacaaatcaa tttgttatca tcaatggcga
    50701 tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt gccgccgttt tcagtttttt
    50761 ctgctggcga gtttatcgct ggtgacacat gctgcgttgt gtcaggatat tgacgcagat
    50821 ttcatgtcga ggactaccat gaacaagttt ttatttgccg ctgcattgat cgtcagtggc
    50881 ctgctcgttg gctgtaatca actcacccaa tacaccatca ccgaacaaga aattaaccag
    50941 tcgcttgcga aacataataa tttctcaaaa gatatcggtt tacccggcgt ggctgacgcc
    51001 catattgttc tgacaaactt gaccagccaa attggtcgcg aagagccgaa taaggttacc
    51061 ctaaccggag acgccaatct ggacatgaac tccctgttcg gtagtcagaa agcgaccatg
    51121 aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa aaggtgcgat cttcctgaaa
    51181 gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa tgcaaacggt gatgcaaacg
    51241 ttgcttccct atttgaacca ggcattacgc aattacttta accagcaacc tgcttacgtc
    51301 ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa aactggcgaa aggcattgaa
    51361 gtgaagccag gtgaaattgt cattccgttt acggattaat gacgagggcg cattcgcgcc
    51421 ctttattttt cgtgcaaagg aaaacatttc cgcttatcct ttgtgtccgg caaaaacatc
    51481 ccttcagccg gagcatagag attaatgact gcaccatccc aggtattaaa gatccgccgt
    51541 ccagacgact ggcaccttca cctccgcgat ggcgacatgt tacaaactgt cgtgccgtat
    51601 accagcgaaa tttatggacg ggctatcgta atgcccaatc tggctccgcc cgtgaccaca
    51661 gttgaggctg ccgtggcgta tcgccaacgt attcttgacg ccgtacctgc cgggcacgat
    51721 ttcaccccat tgatgacctg ttatttaaca gattcgctgg atcctaatga gctggagcgc
    51781 ggatttaatg aaggcgtgtt caccgctgca aaactttacc cggcaaacgc aaccactaac
    51841 tccagccacg gcgtgacgtc aattgacgca atcatgccgg tacttgagcg catggaaaaa
    51901 atcggtatgc cgctactggt gcatggtgaa gtgacacatg cagatatcga catttttgat
    51961 cgtgaagcgc gctttataga aagcgtgatg gaacctctgc gccagcgcct gactgcgctg
    52021 aaagtcgttt ttgagcacat caccaccaaa gatgctgccg actatgtccg tgacggaaat
    52081 gaacggctgg ctgccaccat cactccgcag catctgatgt ttaaccgcaa ccatatgctg
    52141 gttggtggcg tgcgtccgca cctgtattgt ttacccatcc tcaaacgcaa tattcaccaa
    52201 caggcattgc gtgaactggt cgccagcggt tttaatcgag tattcctcgg tacggattct
    52261 gcgccacatg cacgtcatcg caaagagagc agttgcggct gcgcgggctg cttcaacgcc
    52321 ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga tgaatgctct gcagcacttt
    52381 gaagcattct gttctgtaaa cggcccgcag ttctatggct tgccggtcaa cgacacattc
    52441 atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca tcgcactgac tgatgacacc
    52501 ctggtgccat tcctcgccgg ggaaacggta cgctggtccg ttaaacaata aaaatcgtag
    52561 cttcctgttg tcattaggtt attttacctg tataaataac cagtatattc aacagggggc
    52621 tattatgcga attgaagtca ccatagcgaa aacttctcca ttgccagctg gggctattga
    52681 cgccctggct ggcgaacttt cccgccgtat tcagtatgcg tttcctgata atgaaggcca
    52741 cgtatcggta cgttatgccg cagcgaataa tttatcggtt attggcgcaa caaaagaaga
    52801 taaacagcgc attagcgaaa ttctccagga aacgtgggaa agcgccgatg actggtttgt
    52861 cagcgaataa tatgcagtga tttttgtttt tgccgggtcg ccccggcttt tttattttac
    52921 ttacgaattt tgagtacaaa atttctacac ttcctcaaaa taattgcaca atatgctaaa
    52981 aaaatatgaa tgacatgctg ttttattgtt catataaatc ttaaaagcag aaatttatct
    53041 gttcccccct ttaaaaattc ggtgaatacc cttacttatt ggtatactga agttgcttca
    53101 gataaaaacg cattgaacct cgaataacgt tgtctagtaa cacgaattag ggggccatga
    53161 tggaaaaaaa taatgaagtc attcagactc atccgctcgt agggtgggac atcagcaccg
    53221 ttgatagcta tgatgcgctg atgttgcgtt tgcactacca gaccccaaat aagtccgagc
    53281 aggaagggac tgaagttggt cagacgctct ggttaaccac tgatgttgcc agacaattta
    53341 tttcgatatt agaagcagga atcgccaaaa ttgaatccgg tgatttcccg gtaaacgagt
    53401 atcggcgtca ttaacatgac atgaccatcc acccaaaagg cacctcaccg gtgcctttac
    53461 catttctggg ttgtatatct gcattcagtt aattactctg cttaaaccat gagagcagag
    53521 agaaaaaaat gaaatacgat ctcatcatta ttggcagcgg ttccgtaggc gctgccgccg
    53581 ggtattatgc aacccgcgcc ggtttaaacg tgctaatgac cgacgcccat atgccaccgc
    53641 atcaacacgg cagccaccac ggcgatacgc gattaattcg ccatgcttat ggtgaaggcg
    53701 aaaagtatgt cccgttagtc ctccgcgcgc aaacgctgtg ggatgaactt tcccgccaca
    53761 acgaagatga tcccattttt gtacgctctg gtgtcattaa cctcggcccg gctgactccg
    53821 catttctcgc caacgtcgcc cacagcgccg aacagtggca actcaacgtt gaaaagctcg
    53881 atgcgcaagg gattatggcc cgctggccag aaatacgcgt cccggacaac tacatcggct
    53941 tatttgagac tgattccggt tttttgcgca gcgaactggc gattaaaacc tggatccaac
    54001 tggcgaagga agcgggctgt gcgcaactgt tcaactgccc ggtcaccgca attcgtcatg
    54061 acgatgatgg cgtaactatt gaaacggttg acggtgagta tcaggcgaaa aaagcgattg
    54121 tctgcgcggg aacatgggta aaagacctgc tcccggagct gcctgtccag cccgtacgta
    54181 aagtatttgc ctggtatcag gccgatggcc gctatagcgt gaagaataaa ttcccggcgt
    54241 ttaccggtga actgcccaat ggcgatcaat attatggttt tccggcagaa aacgacgcgt
    54301 tgaagattgg caaacataac ggaggccagg ttatccattc agcggatgaa cgtgttccgt
    54361 ttgcggaagt ggccagtgat gggtcagaag ccttcccgtt cttgcgcaat gtattgccgg
    54421 gtatcggttg ctgcctgtac ggcgctgcct gcacctatga taattcgcct gacgaagatt
    54481 ttattatcga taccctaccc ggccacgata atacactgct cattaccggc ctgagtgggc
    54541 acggttttaa atttgcgtca gttttagggg aaatagctgc cgattttgcg caagaccaaa
    54601 aaagcgattt tgatttgacg ccattcaggc tttcccgctt ccaataatca tcattacggc
    54661 ctccagaatg aggccgtttt tacaggagta tttaatgcgt cggctgttgc actatctcat
    54721 caataatatt cgcgagcatc tgatgcttta tctttttctc tggggattgt tggcaattat
    54781 ggatctgatc tatgtgtttt atttttagtt ttccgcactg acataaactg acaaatattt
    54841 aatctccctt tcatttagcg tgaaaaattg aatgagatta ttcctgcttc tacagcactc
    54901 accagacatc tctgatgctg aacccactaa acttatgaag attctttcat tattaattca
    54961 aaattattga aataaaaata aatatcattc aatttgttat ttttgtgttg caattcattc
    55021 tccgacacgc cattttcttt ctctcgcaaa ttcgtattgg atgcacttat gtcattcaca
    55081 aatacccctg aacgctatgg tgttatatct gcggccttcc attggctaag cgcaattatt
    55141 gtctacggca tgtttgcttt aggtctgtgg atggtcacgc tcagttatta cgatggctgg
    55201 tatcacaaag cacccgaact gcataaaagc atcggtattt tgttaatgat ggggctggtt
    55261 attcgcgttc tgtggcgtgt catatctcca ccgccgggtc cgctgccgag ttattcgccc
    55321 atgactcgtc ttgccgcaaa agctggacat ctcgcattgt acctattgct gttcgccatc
    55381 ggcatcagcg gctatctgat ctcaactgcc gatggtaaac cgatcagcgt ttttggctgg
    55441 tttgacgtcc ccgcgaccct tgccgacgct ggcgcacagg cagactttgc cggtgccctg
    55501 catttttggc tcgcgtggag cgtcgtagta ctgtctgtta tgcacggatt tatggccctg
    55561 aagcatcatt tcatcgataa agacgacact ctgaagcgca tgctgggaaa gtcgtcatct
    55621 gactatggag tataaaaaat gaaaaaaagc ctgcttggtt taaccttcgc gtccctgatg
    55681 ttctctgccg gttcagcggt tgccgccgat tacaaaattg acaaagaagg tcagcacgcc
    55741 tttgttaact tccgcataca gcatcttggc tatagctggt tatatggcac ctttaaagat
    55801 ttcgacggta cttttacctt tgatgaaaaa aatccggctg ccgataaagt gaatgtgaca
    55861 attaacacca ccagcgtcga tactaaccac gccgaacgcg ataaacatct tcgcagtgcg
    55921 gattttctca ataccacaaa atatccacag gcaacattca cctccaccag cgtgaagaaa
    55981 gacggtgacg aactggatat taccggcgat ctgacgctga atggcgtaac caaacccgtc
    56041 acgctggaag cgaaattaat tggtcagggc gacgacccat ggggcggtaa acgtgcaggc
    56101 tttgaggccg aaggcaaaat taagctcaaa gacttcaata tcaagacaga tttaggtcca
    56161 gcttctcagg aagtggatct gattatttca gtggaaggcg tacagcagaa gtaagagtga
    56221 aatgttgccg gatggcacgc atccggcaat gatatttatt ctgttggatc aggaataccc
    56281 agtgttgtat tcagacgtcc acgagactta ttaaagatct tattgccatt ttcacgtcct
    56341 gcccgacggc gtcgctgttc ttctggcggt aacgcgcttt cttcgcagca aatctcacta
    56401 caacaacctt tgtatttttc cgcgcatact ggacactgaa taaacagcag gtggcagcca
    56461 tcatttttac agttggtatg gctgtcgcac ggcgcgccgc actggtggca atgcgcgata
    56521 atctcatccg atatacgttc gcccatccgc tcgtcaaaaa caaaattttt gccaataaaa
    56581 cgcaccggca agccctgctc gcgcgcctta cgggcgtatt caataattcc gccctcgata
    56641 tgccagactt tgttgaatcc gttatgtttc atccaggcac tggccttttc acaacgaatg
    56701 ccgccggtgc agtacatgac gattttttta tctttatgtg cctgcatcat ctcgactgct
    56761 tttggcagct gctcacggaa ggtatctgcc ggaatttcga gcgcgttttc aaagtgcccc
    56821 acttcatact catagtggtt acgcatgtcg ataaatagtg catcgggatc gtcaagcatg
    56881 gcgttcactt ccgccgcttg cagatactca ccaacattgc tggcatcaaa gtgaggatcg
    56941 tcaataccgt cggcaacaat gcgatcgcgt accttcatgc gcagtaccca gaaggatttc
    57001 ccgtcatcat ccaacgcgat attcaggcgt aaaccctcta aagccgggtc gaaggcatag
    57061 agctgcgcgc gaaatgtttc aacattgctc gcaggtacgc tgatttgcgc gttaatgccc
    57121 tcatgcgcca gatacactcg cccaaaaaca ttcagcgcgg taaacagctg atataaagcg
    57181 tcacgggtcg ccttaggatc ggcgatgtgg aaatacttgt aaaacgaaat ggtggttcgc
    57241 ggttcgctct cagccaacat tttggctttt aacgcgtcgt tggaaatgcg gttgtgtaac
    57301 actggcatgg tgtacggttc ctgcgagatg ggaaagtaaa aatccgcggc atgatatagc
    57361 aattatcgat aattaacatc cacacatttt acgctacatt tgcgcattaa aaattatttg
    57421 ttatttacaa gcgcggcaat ttcgcccagt cttcagccac aaattttggt tgcgggcgaa
    57481 aaaatgcgac aatacataca attgcccgta taggttgaaa aacaggattg atatgacgaa
    57541 tctacccaag ttctccaccg cactgcttca tccgcgttat tggttaacct ggttgggtat
    57601 tggcgtactt tggttggtcg tgcaattgcc ctacccgatt atctaccgcc tcggttgtgg
    57661 attaggaaaa ctggcgttac gttttatgaa acgacgcgca aaaattgtgc atcgcaacct
    57721 ggaactgtgc ttcccggaaa tgagcgaaca agaacgccgt aaaatggtgg tgaagaattt
    57781 cgaatccgtt ggcatgggcc tgatggaaac cggtatggcg tggttctggc cggaccgccg
    57841 aatcgcccgc tggacggaag tgatcggcat ggaacacatt cgtgacgtgc aggcgcaaaa
    57901 acgcggcatc ctgttagttg gcatccattt tctgacactg gagctgggcg cgcggcagtt
    57961 tggtatgcag gaaccgggga ttggcgttta ccgcccgaac gataatccac tgattgactg
    58021 gctacaaacc tggggccgtt tgcgctcaaa taaatcgatg ctcgaccgca aagatttaaa
    58081 aggcatgatt aaagccctga aaaaaggcga agtggtctgg tacgcaccag atcatgatta
    58141 cggcccgcgc tcaagcgttt tcgtcccgtt atttgccgtt gagcaggctg cgaccacgac
    58201 cggaacctgg atgctggcac ggatgtccgg cgcatgtctg gtgcccttcg ttccacgccg
    58261 taagccagat ggcaaagggt atcaattgat tatgctgccg ccagagtgtt ctccgccact
    58321 ggatgatgcc gaaactaccg ccgcgtggat gaacaaagtg gtcgaaaaat gcatcatgat
    58381 ggcaccagag cagtatatgt ggttacaccg tcgctttaaa acacgcccgg aaggcgttcc
    58441 ttcacgctat taaatctccc atgccggatg cttcagaatg gcatccggca ttaccacagc
    58501 aaatccccct gatttagcga taaaagctct ctggattgcg ccccctggaa gtcgggcgca
    58561 taattagtgt gtttatcttt tcttcttatg ttcaccgcgc ctggcgcacc aacagcggat
    58621 tgctatgtca ccctgtgaaa atgacacccc tataaactgg aaacgaaacc tgatcgtcgc
    58681 ctggctaggc tgttttctta ccggggccgc cttcagtctg gtaatgccct tcttacccct
    58741 ctacgttgag cagcttggtg ttaccggcca ctccgccctg aatatgtggt ccggtattgt
    58801 cttcagtata atgggttcct aaacacatca ttttagatat tgaatatgag acgggcaatc
    58861 agctacatca gattttcaag tgaacgacaa ctgaaagggg actctgtacg ccgacaatct
    58921 aagctggtta ctgactggct tgataagaac cctgagtttt acttggattc atccttgagc
    58981 tttaaagatt tggggaaaag tgcttttagt ggtaagcacc tgaaaggtgg tctaggtgac
    59041 tttttaaccg ctatcgagaa agggcttgtt aaagctggtg atacgctact aatcgagagt
    59101 ttagaccgtc tgtcacgtca ggatattgat attgcttctg agctattacg ccgtatctta
    59161 agggctggtg tagatgtagt cacactgagc gatggagaac attacacacg agaaagttta
    59221 aaagacccgt tagccctgat taaaagcatt ctgataatgc agcgggcgca tgaggaaagt
    59281 ttgaggaagt ctgagcgtgt ccaagctgca tggaaccgta aaaaagaact catctccgaa
    59341 ggtatcaagg tatctaggcg ttgtcctgca tggttaaggc tgaatgatga ccgtcgaaca
    59401 ttcacaatca ttcctgataa agtggaagtt gttaaacgag catttgacct gagattacag
    59461 ggtttaagct tttgggctat caccagaact ttaaacgatg aaggtcactt gtcattgaat
    59521 caatacacac ccaaacaaaa ggggtggtct gatactgctg taaagaaact actcagaaat
    59581 cgtgctgtga ttggatgttt cacacctgct ggacgtgaag aagttcaggg ttactacccc
    59641 gcaataatca gtgagtcact attctatcga gtacagcaac tgaacacagg gcaatatggt
    59701 agggcttctg tatcaagtaa tccgctatca gtcaacttgt tcaggggtat catcaaatgt
    59761 tctgagtgtg gggctactat tgttcttggt ggatatgcct taaaacgttt tggaatgtac
    59821 agatgcccta atcgttcagc taacaggtgt agtgcaaagg ctatcagtcg taagcaaacg
    59881 gatgcaacta tcctttacat gctcgcatta tgcgaccgtt ttgaaactga aaatactgac
    59941 actattgata gccttaaatt acagcgtgaa gatttg
//
LOCUS       DADMGC010000008        58005 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.008, whole genome shotgun sequence.
ACCESSION   DADMGC010000008 DADMGC010000000
VERSION     DADMGC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.008"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(24..278)
                     /locus_tag="JNZ73_000514"
     CDS             complement(24..278)
                     /locus_tag="JNZ73_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAY8730914.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="JNZ73_000515"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="JNZ73_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HAY8730915.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="JNZ73_000516"
     CDS             1228..2694
                     /locus_tag="JNZ73_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY8730916.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNPLKI"
     gene            2812..3798
                     /locus_tag="JNZ73_000517"
     CDS             2812..3798
                     /locus_tag="JNZ73_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAY8730917.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQLL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="JNZ73_000518"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="JNZ73_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HAY8730918.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="JNZ73_000519"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="JNZ73_000519"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HAY8730919.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAMG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="JNZ73_000520"
     CDS             5709..7265
                     /locus_tag="JNZ73_000520"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY8730920.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVARHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHLVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="JNZ73_000521"
     CDS             7347..9161
                     /locus_tag="JNZ73_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY8730921.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWHFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="JNZ73_000522"
     CDS             9162..10256
                     /locus_tag="JNZ73_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HAY8730922.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YMFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="JNZ73_000523"
     CDS             10256..11281
                     /locus_tag="JNZ73_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8730923.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="JNZ73_000524"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="JNZ73_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HAY8730924.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     LSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="JNZ73_000525"
     CDS             complement(12876..13220)
                     /locus_tag="JNZ73_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8730925.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="JNZ73_000526"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="JNZ73_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HAY8730926.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAVFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="JNZ73_000527"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="JNZ73_000527"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HAY8730927.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="JNZ73_000528"
     CDS             15616..17376
                     /locus_tag="JNZ73_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAY8730928.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDPPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="JNZ73_000529"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="JNZ73_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HAY8730929.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="JNZ73_000530"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="JNZ73_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HAY8730930.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="JNZ73_000531"
     CDS             19113..19340
                     /locus_tag="JNZ73_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HAY8730931.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="JNZ73_000532"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="JNZ73_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HAY8730932.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDTVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="JNZ73_000533"
     tRNA            21195..21271
                     /locus_tag="JNZ73_000533"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="JNZ73_000534"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="JNZ73_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HAY8730933.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="JNZ73_000535"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="JNZ73_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HAY8730934.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGNKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /locus_tag="JNZ73_000536"
     CDS             complement(25143..26195)
                     /locus_tag="JNZ73_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HAY8730935.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="JNZ73_000537"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="JNZ73_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HAY8730936.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /locus_tag="JNZ73_000538"
     CDS             complement(26746..28689)
                     /locus_tag="JNZ73_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY8730937.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="JNZ73_000539"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="JNZ73_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HAY8730938.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="JNZ73_000540"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="JNZ73_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HAY8730939.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="JNZ73_000541"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="JNZ73_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HAY8730940.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="JNZ73_000542"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="JNZ73_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HAY8730941.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="JNZ73_000543"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="JNZ73_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HAY8730942.1"
                     /translation="MLEAREVLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="JNZ73_000544"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="JNZ73_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HAY8730943.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="JNZ73_000545"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="JNZ73_000545"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HAY8730944.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="JNZ73_000546"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="JNZ73_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HAY8730945.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="JNZ73_000547"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="JNZ73_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HAY8730946.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="JNZ73_000548"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="JNZ73_000548"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HAY8730947.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTAMKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="JNZ73_000549"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="JNZ73_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HAY8730948.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="JNZ73_000550"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="JNZ73_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HAY8730949.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRVWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="JNZ73_000551"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="JNZ73_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HAY8730950.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38328..39952)
                     /gene="mqo"
                     /locus_tag="JNZ73_000552"
                     /pseudo
     CDS             complement(38328..39952)
                     /gene="mqo"
                     /locus_tag="JNZ73_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
     gene            complement(40170..41813)
                     /gene="yojI"
                     /locus_tag="JNZ73_000553"
     CDS             complement(40170..41813)
                     /gene="yojI"
                     /locus_tag="JNZ73_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HAY8730951.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVPLLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41889..42483)
                     /gene="alkB"
                     /locus_tag="JNZ73_000554"
                     /pseudo
     CDS             complement(41889..42483)
                     /gene="alkB"
                     /locus_tag="JNZ73_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
     gene            complement(42483..43547)
                     /gene="ada"
                     /locus_tag="JNZ73_000555"
     CDS             complement(42483..43547)
                     /gene="ada"
                     /locus_tag="JNZ73_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HAY8730952.1"
                     /translation="MKNATCLTDDQRWQSVLARDPDADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43621..44676)
                     /gene="apbE"
                     /locus_tag="JNZ73_000556"
     CDS             complement(43621..44676)
                     /gene="apbE"
                     /locus_tag="JNZ73_000556"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HAY8730953.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44788..45909)
                     /gene="ompC"
                     /locus_tag="JNZ73_000557"
     CDS             complement(44788..45909)
                     /gene="ompC"
                     /locus_tag="JNZ73_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY8730954.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46647..49319
                     /gene="rcsD"
                     /locus_tag="JNZ73_000558"
     CDS             46647..49319
                     /gene="rcsD"
                     /locus_tag="JNZ73_000558"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HAY8730955.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQILIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPRIEKYISDIDSYVKSLL"
     gene            49336..49986
                     /gene="rcsB"
                     /locus_tag="JNZ73_000559"
     CDS             49336..49986
                     /gene="rcsB"
                     /locus_tag="JNZ73_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HAY8730956.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGLLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50186..53035)
                     /gene="rcsC"
                     /locus_tag="JNZ73_000560"
     CDS             complement(50186..53035)
                     /gene="rcsC"
                     /locus_tag="JNZ73_000560"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HAY8730957.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKKTLTVYAERVRKSRDS"
     gene            complement(53310..54086)
                     /locus_tag="JNZ73_000561"
     CDS             complement(53310..54086)
                     /locus_tag="JNZ73_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HAY8730958.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQISGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDMDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54091..55740)
                     /locus_tag="JNZ73_000562"
     CDS             complement(54091..55740)
                     /locus_tag="JNZ73_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HAY8730959.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVSSMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPGSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55741..58005)
                     /locus_tag="JNZ73_000563"
                     /pseudo
     CDS             complement(55741..58005)
                     /locus_tag="JNZ73_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
BASE COUNT        14166 a        15661 c        14266 g        13912 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccatc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcggccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaacc ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gctcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttgtg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcctggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcaatg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gccaggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact tggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcgcccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggca tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgcct atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcgc tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctag gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcg tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatatgt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatgct gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcgcttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaaca
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cagcgcactg atgaccatcc
    13861 acgctgccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgaccccccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctgaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcagatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccggttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgcccg cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct ggtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagaca ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ttggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcggt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atagcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ctttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggtc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata atgaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgacggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcaacaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gtcaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaggtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcca gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggt
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtacctctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc aaggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgttg gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac ggttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcatggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag accctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcgaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcaaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct tgtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgca gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatccg gcataccatg ccggatgtgg cacatcatta caacgcaata
    38341 tccgccactt ctttttgaac gggttgcggt ttcaactgcg gtttcggcgt actatctgct
    38401 gcttgcggtt tgtcgtattt cagccccagc acttcgctgg tgtactgcaa ctcgcgttct
    38461 gttgccgcta catcaccgtt cagcttgcgt ccataagacg gaacgatcgc tttcaacgta
    38521 gactgccatt gcgggctgga aacgcgatcg ccaaatactt tttccagcag attcaacata
    38581 atcggcgcgg cggttgacgc ccctggcgat gcccccagca gcgccgcaat ggtcccttgc
    38641 tggtcactga ccacttcggt acccagacgc agtacgccac ctttctcggc atcacgcttg
    38701 ataatctgca cacgctgccc cgcttgccac aaacgccagt cctctttttt cgcttgcgga
    38761 tagtactctt tcaacgcttc aaaacgatcc tcttcactca acatcacctg actcaccaga
    38821 tatttcacca gatcgaaatt atccagcccg acgtgcatca tcggcatcac gttagaggtg
    38881 gtggtggaac tcatcagatc ccacaatgaa ccgtttttga ggaatttggt tgagaaggtg
    38941 gcaaatggcc caaacagcac tacgcgttta ccgtccagaa cacgggtatc gatatgcgga
    39001 accgacatcg gtggtgcgcc aacggatgct ttaccgtaaa ctttcgccag atggcgatta
    39061 accacgtctg gattttccga aacaaggaac tgtccgccca ccgggaaacc ggcgtagtct
    39121 ttcgcttccg gaatccccga ttcctgtaac agcttcagcg ccgcaccgcc cgcgccgata
    39181 aagacaaatt tggtgcggat gttctgtgca gtgccgtttt tcagatcggc aacggtaacg
    39241 gtccaggtgt tatcgtcatt acgctttagg gcgcggactt cgctgctgag ttgcagcgag
    39301 aagttagatt tcttctgtaa ggaagcaatt aactggcggg tgatctcacc atagttcaca
    39361 tcagtgccaa tttctgttgc ggatcgcgcc cttccatcac taacggtgcc cactctttga
    39421 tctgcgcgtg atcttcagag taacgcatac cgcgaaacag cgagctttgt tgcaacgcgg
    39481 cgtaacgggc gcgcaggaaa ttaacgttat cctcgcccca gacaaagctc atatgcggaa
    39541 cggtattgat aaatgaacgc ggagtacgca gcacgccgcg ctcgacctgg tgcgcccaga
    39601 actggcggga aatctgaaat gcttcgttaa tggcgactgc tttttcaata ctgatgctgc
    39661 catcggcgtt ttgcggggtg tagttcagtt ccatcagtgc agaatgcccg gttccggcgt
    39721 tgttccagcc gttcgaactc tcctgcgcgc ccccctccag gcgctccacc atggtcatcg
    39781 accattcagg ctccagctcg cgtaaatagg tccccaacgt ggcgctcata atgccgccgc
    39841 caatcaacag tacatcagtt tcctgctcct cggacgcttt cgcttttgcc gccatcgaaa
    39901 cggcattaag ccccacggcc atcgagaaga gcatggcagt cacttttttc atcttgttaa
    39961 tgccttactt ttagtcgctt tattgcaggt gagatttgcg cggcatcaac ggtaacactt
    40021 aagtaacaac atttaaataa tgtttataaa ttatattcta attttagaaa cattataatt
    40081 tcgttgatta attatagggt tattagaaat gaagggattt ttagcatatt gacctgacgg
    40141 cagcagtccg ccaggtcagc gtcgtaatat tatgccgtcc gggcaacggc atcacgcgaa
    40201 gcggcatcgc gctcttcgcc cgtcagctcg ctaagttgcc cattgcgcat ttccagcagg
    40261 cggtcggcgt ggataaagta atgatcatca tgactgatag cgaaaatagt tttacccatc
    40321 tcctgcatca gcggcagcaa cacctgataa aactcacgac ggaagtgtgg atcctgatcc
    40381 gccgcccatt catccagcag gataatatcg cgttcttctg ccagcgccag caacagcggc
    40441 acgcgttttt tctgcccttt tgataacttc aggttaacaa tacgcccgtt gcttaactca
    40501 agcttatgag ccattttcag ctgcgccagc cacttctcaa ccagttgcgg gttagcgggt
    40561 tgcccctccg gccccagcag ttgatcaaac agccagacat cggtaaacac tgccgaaaac
    40621 agtttgcgat aatcttccgg ttgttcgccg ctgacaggtt tgccatccag caagatttcg
    40681 ccgctttgtg gctgatacaa gcccgtcaac aacatcgcca gcgtcgattt tccgctaccg
    40741 ttgccgccaa tcagaaacag cagctcgcca cgtttgatgg tgagattaat cggaccaacg
    40801 gaaaacgcgt tatcctgata agcaaacgtc acgttacgca gctccagcgt ttgccagttg
    40861 ggaaacgcct gcgggcgcgg aaactcagct ttgaaaggcg cgagcgcgaa tttgttcagc
    40921 ttgttaaacg ccacctgcgc cgtcagcagc gtcggcaatg cgccaaccgc cgaaagcagc
    40981 ggcgtacgca ggaataacag cgtcaacgaa taggtcgcag caacgttggt atcagcccaa
    41041 ccgagactgt tcgccatcca gaacaccagg ccgatagcgc ccagcatcat gatgtttgac
    41101 cagttcacgg cactaagatg gaaggtgtct gcgcgaataa tatgatggcg atactcttgc
    41161 gcatcgggga tgtagaggtt gttaaacaca tactcggcgc gttcccggtt cagagtcagc
    41221 tctttgcgcc cttcaagtac agtttgaaaa tccgtgtaca gcttgtcttc ggtttcacgt
    41281 agggtcgcca tatgtttgta cacccgcgcc accagcacaa aaccgcccca gatggtgatc
    41341 gccatccaga tagccgttac cagcaacatt ttgcccgaca gcatccacag atacgccgat
    41401 gaaccgatag tgagaatgat cccctgcccc agttccggca gacgcacaaa agcaatggtg
    41461 atattgcgca catcgctggt taatcccgcc agcaacgagg cgctaccgag ttgttcaatg
    41521 cgctcgacgt gggtatccag aatccgcttg ataaattcac tacgcagtcg gtaaacgaag
    41581 tgatgcccca aagtggtgag cgccagttgc gatccgagag tgactgccat caacagcagc
    41641 aataatccca gaaactccgg taacaccagc agactggtat ccgccgtttc gataaggcgc
    41701 tgattgataa aagcaattaa gccaatgcct aatgccgcac tggcgaggct tagcgccatc
    41761 acactgataa atggccagcg atactgccgc cagacaagta caagaagttc catgcagaaa
    41821 acccggacaa tgaattacag cccgcagttt aaacatcttc gcgcgcacag caataataat
    41881 tcttattttt attctttttt acctgcctga cggaatgtca ggttgtagcg gcagtcggtg
    41941 gtgagtggat gaaaccccgc tttcaacggt tgaataccgt gataaaacag ccgcgattca
    42001 ccgccccagg ccaccaaatt gaaaaatcgc gggtaagccc agagaaacag aaacaattgg
    42061 cgcgcgcaga tccggttcgt ctttatcctg atgcagcgac agtttcgcgc taggagcgta
    42121 gcggttgata agacaagcat ctggctggaa atctggatag cccgccgccg tagccgcacg
    42181 ttgacataaa tcatgaaagc tttgtggcat ggcgggccac ggtttatttg tttgcggatc
    42241 aatgggcgaa tagagataac cttgccgatg ggtcgtccag cccagatgcc cacagttggt
    42301 catcgccacc gacatggtat atccgcctgg agcgaccatc tggcgaaacg gcgactggct
    42361 ggcaacgtca ttaatatcgc ggattagttg ctccgcagca ttaaaagcaa aacgccgtaa
    42421 aattaccgca ccagccgcca gtggctcttg ccacggttca gcatcggcaa acagatccaa
    42481 cattacctct cctcattttc agcttcgcgg cgcagcagtt gcgctttacg cgacacgccc
    42541 cagcggtaac cggaaagtgt gccatcacca cggaccactc gatgacaggg tattacgata
    42601 gccagcttgt tggcggcaca ggcgctggca acggcccgta ccgctttcgg tttgccgatg
    42661 gcgttagcca gttgctgata actgacggtt tcaccacaag gtatcgtgcg cagtgcctgc
    42721 cagacttgtt gctgaaaagc agtgccgcga atatccagcg gtaacgtcag cggcgtatcg
    42781 cgttgattga ggctggcgat cacttcacgc acatgttgct gaaacgtcag atcggcaggc
    42841 gcgttgtcgg cagcgggaaa catctgctgc aactcgctga ttaatgtcgc gtcatcatcg
    42901 cccagcaata tcgcgcaaat cccccgctcg ctttctgcca ccaggcaacg acccagctca
    42961 caatcagcca gcgcgtaacg caccgccaga ttttcgccac cgtgacggaa ttgtttagcc
    43021 gtcatgccca gcgtttcatc ggctttgcgg tagtaactgc tgctgtcggg gaatccggcg
    43081 ttaagaatag acgtcgtcac gctctcccct ttcgccagcg attcgcgcaa acggcgagcg
    43141 cgccaggcct gttgccaggc tttaggcgtc attccggtag tagctttaaa caaccgatgt
    43201 agatgaaatg gactcatcgc cacctggtcg gctaaggctt ccagcgttac aggcgtttcc
    43261 tgttccagca gtcgacacgc gtgggtgatt ttatccaacc gatgttgccg gggattggct
    43321 ttgtctggct gacaacgttt gcaggggcga aatccggcgg cgagtgcctc gctggcattt
    43381 gcgtagaagg agacattttc ccgcaaggca tgtctggcgc ggcaagacgg acggcaaaag
    43441 atacccgtgg tgcgcacggc gaaaacgaat tcgccgtcgg catccgggtc gcgggctaag
    43501 acagattgcc agcgttgatc gtcagttaag catgtggcgt ttttcataat cagctccctg
    43561 gttaaggata gcctttagtc tgcccggtca ccatcacgca aaaaccaaca atcttgcgct
    43621 ttaatttttt tcgctgacaa ggaagctttt aaactgtggt gacatccagg ttttaaagct
    43681 atcgccttct ttggtgatca tataaaccgc cagcccttcc cggcgaacaa cctctttggc
    43741 tttctccggc ccgagtacca tcaagccagt atcccaggca tcggcttcca gcgccgtcgg
    43801 agcaatcacc gtcacggata ccagattgtg ttcgatggga cgcccggttt gcggatcgat
    43861 aacatgggaa agacgtttgc cgtccagttc gtaatagtta cggtagctgc cagaagtgct
    43921 aatcccatga ccgttgatat ccaccacagc ctgaaccgcg ttttctttat cggttggttt
    43981 ttgaatcgct acccgccacg gctggccttc accgttcata ccacggctgt tcagcgcgcc
    44041 gcccaccgac accagatagc gggaaatccc ttcctgctcc atcaagcgtg ccaggtgatc
    44101 cgccgcataa ccttcaccga cggtggagag atcgacatat aaatccggca ggtctttttg
    44161 cagatattgc tgatgcgact gattgatgac cgtcaggtgc tgtaagccgg ttttggcttt
    44221 catcgcatcg atctgttcct ggctcggaat ttgaaccggc tgttgttccg ggccaaagcc
    44281 ccacagattc accagcggcc cgacggtaat atccatcgca ccatcggtcc ttgccccaat
    44341 gcgcagcgag gtggtgacga tatcggccat cgcttcactt accggccacg gcgacaaact
    44401 ttgcgagtcg ttaaagcgca tcagcgcgga atctttttta taggtcgaaa gcagctgatc
    44461 gtcggcgtcc agctgggtct gaatcttttc tttaagttcg gcactgcgtt tggcatcaat
    44521 gcccgggatg ctggcacgcc agaaggtacc catggttttg ccttcaagaa cggtaacttc
    44581 agtagcgtgg gttttggcgg gttgtggttt ttgatcgcaa ccaacaaaga agagcgcggc
    44641 aaccagcagt gccacgcggg taaagcttat ttccatacgt gattatcctc atgcgaacgg
    44701 tcgcaagagt acaccaaaaa actgtgtttg tacgctgaaa acaatgaaaa aagggcccgc
    44761 aggccctttg ttcgatatcc atcgagatta gaactggtaa accagaccca gagctacgat
    44821 gttatcagtg ttgatgccag cgtcacgagt gaactggttg tcgtccagca ggttgatttt
    44881 gtagtcaacg taggtggaca tgtttttgtt gaagtagtag gtcgcaccaa catcaacata
    44941 tttcaggata tcttcgtcgt cgtagttacg accattgatg acacccaggt ttttaccttt
    45001 agactgcagg taagctaggg acggacgcag accgaagtcg aactggtact gagcaacagc
    45061 ttcgaagttc tgtgctttgt tcgcccaacc caagttgcct acgcgagttg cgttgtaggt
    45121 ctgggtgtac tgagcagcta ggtagatgtt gttcgcgtcg tatttcagac caccggtgta
    45181 ggtttcagcg cggtcgccat taccgatgta acgctcatca taacggttca gaccgaagtt
    45241 ctggtcatca gtacgtttgg agctggaaac tgcagcaccg ataccgaagc cttcgtaatc
    45301 ataagtaata gaaccgccaa cgccgtcacc gttctgacgc agagcttcac gaccgttgtt
    45361 ggtcatgcct tcaccttccg ggctaccgtt tttgccctga tactgaacag cgaagttcag
    45421 accgtcaacc agaccaaaga agtcggtgct acggtaggtt gcgaagccgt taccacgctg
    45481 ctgcatgaag ttgtcagaac cgtaagtgtc accaccgaat tctggcagta cgtcggtcca
    45541 ggaagttacg tcgtaaacta cgccgtagtt acgaccgtag tcgaaagaac ctacatcctg
    45601 gaatttcaga cctgcgaatg ccacacgggt ccaggagttg ttttcgtttt cagcgctgtt
    45661 gccctggatc tgatattccc actggccgta accggtcagc tggtcagtaa cctgagtttc
    45721 acctttgaag ccaagacgca tgtaggtctg gtcgccatct acagacttgt cgtcagagaa
    45781 atagtgcagg ccgtctactt taccgtacag atctaatttg ttgccgtctt tgttgtaaac
    45841 ttcagcagcg tttgctgctc ctgctaccag cagagctggg accaggaggg acagtacttt
    45901 aactttcatg ttattaaccc tctgttatat gcctttattt gcttttttat gccactgcat
    45961 actgattaac cctcattaat cagtcggcaa gtccattctc cccaaaaatg cagaataatc
    46021 caacacgaat atgatactaa aacttttaag atgtttcatt tatcgctaca gatgtttcaa
    46081 aatgtaaata caagggaact ttttaagatt attgcggaat ggcgaaataa gcacctaaca
    46141 tcaagcaata ataattcaag gttaaaatca ataacttatt cttaagtatt tgacagcact
    46201 gaatgtcaaa acaaaacctt cactcgcaac tagaataact cccgctatca tcattaactt
    46261 tatttattac cgtcattcat ttctgaatgt ctgtttaccc ctatttcaac cggatgcctc
    46321 gcattcggtt tttttaccct tctttacaca cttttcatta ttctgtgcta ccacagaaaa
    46381 actataacgc ttgttaacta tttcacaaat aattaacatc agcataattt ccagcaatct
    46441 ttgtttattt acaattaatt tcgttgggct ttttgtaggt tatttgtaca gcaaaatggc
    46501 gcttgtacat ctatttcccc ccatacagga tgataaatat cacgggagaa tagagaatca
    46561 tcaatcaggt aagagtctgg aatttcacac tgtacccttt atactgccct atcacttcgc
    46621 gaagttttaa caggtcataa acacgaatgc gtcagaaaga gacaacggcc acgacccgct
    46681 tttcactcct accggggagc attacccgct tctttttact gttgatcatt gtgttactgg
    46741 tgacgatggg ggtaatggta caaagtgccg ttaacgcctg gctgaaagat aaaagttacc
    46801 agattgtcga cattacccac gcaatccaaa agcgcgtcga tacctggcgt tacgtgacca
    46861 ggcagatcta cgacaacatt gccgcgacga cctccccctc ctccggcgaa ggtttacaag
    46921 agacgcgcct gaaacaggat gtctactatc tggagaaacc acgccgcaaa acggaagcgt
    46981 taatctttgg ctctcacgac aactcaacgc ttgagatgac tcagcggatg tccacttatc
    47041 tggatacatt gtggggtgca gaaaatgtac cgtggtcgat gtattacctg aatggtcagg
    47101 ataacagtct ggtgctgatc tcaaccctac cccttaaaga tctcacctcc ggatttaaag
    47161 aatcgaccgt cagtgacatt gttgattcac gtcgtgcaga aatgttgcaa caggccaacg
    47221 ccctcgatga acgcgaaagt ttttctaaca tgcgccgcct ggcctggcag aacggtcatt
    47281 actttacctt gcgtaccacg ttcaaccagc cgggacatct ggcaacggtc gtggcttttg
    47341 atctgccgat taatgatttg atcccaccgg gtatgccgct ggacagtttc cgccttgagc
    47401 cagacgcgac ggcaacggga aacaatgata atgagaaaga agggacggat agcgtcagta
    47461 tccactttaa cagtacgaag attgaaatct cctcggcact caactctacc gatatgcgcc
    47521 tggtctggca ggttccttat ggcaccttat tgctggatac gttgcaaaac attctgctgc
    47581 cactgctgct gaacatcggt ttgctggcgc tggcgttatt tggctatacc acattccgcc
    47641 atttctccag ccgcagtaca gaaagcgtcc ccagcacggc ggtcaataac gaattgcgca
    47701 ttttacgggc aattaatgaa gagatagtct cactgttgcc gctcggcctg ctggttcacg
    47761 atcaggaatc gaaccgcact gtcataagta acaaaattgc cgatcatttg ctgccgcatc
    47821 tgaatctgca aaacatcacc accatggcgg aacagcatca ggggattatt caggcgacga
    47881 tcaataacga gctgtatgag atccgcatgt tccgcagcca ggtcgcgccg cgcacacaaa
    47941 ttctcattat tcgcgatcag gatcgcgaag tgctggtaaa caagaaactc aagcaggcgc
    48001 agcgtctgta tgagaaaaac cagcaggggc ggatgacctt tatgaaaaac attggcgatg
    48061 cgctgaaaga acccgcacag tccctggcgg agagtgcggc taaactcaac gccccggaaa
    48121 gcaaacaact ggcgaatcag gcagatgtgc tggtgcggct ggtcgaagaa atacagttag
    48181 cgaacatgct tgcggatgat agctggaaaa gtgagacggt gctgttctcc gtgcaggatt
    48241 taattgatga agttgtgcct tcagtgttgc ctgccatcaa gcgtaaaggt ctgcaactgc
    48301 tgattaacaa tcatctgaaa gcacacgata tgcgccgcgg cgatcgcgat gccttacgac
    48361 gtattttgct gctactgatg caatatgccg tgacctcaac gcaattggga aaaatcaccc
    48421 ttgaggttga tcaggatgag tcctctgaag accgcctgac attccgcatt ctggacacgg
    48481 gagaaggcgt aagtattcat gaaatggata atttgcactt cccgtttatc aaccagaccc
    48541 aaaacgatcg ttatggaaag gcggacccgc tggcattctg gctgagcgat caactggcac
    48601 gtaaactggg cggtcattta aacatcaaaa cgcgggatgg gcttggtaca cgctactctg
    48661 tgcatatcaa aatgctcgcc gctgacccgg aagttgaaga ggaagaagag cgtttactgg
    48721 atgatgtctg cgtaatggtg gatgttactt cggcagaaat tcggaatatt gtcactcgcc
    48781 tgttagaaaa ttggggtgca acctgtatca cacccgatga aagattaatt agtcaagatt
    48841 atgatatctt tttaacggat aatccgtcta atcttactgc ctctggcttg cttttaagcg
    48901 atgatgagtc tggcgtacga gaaattgggc ctggtcaatt gtgcgtcaac ttcaatatga
    48961 gcaacgctat gcaggaagcg gtcttacaat taattgaagt gcaactggcg caggaagagg
    49021 tgacagaatc gcctctgggc ggagatgaaa atgcgcaact ccatgccagc ggctattatg
    49081 cgctctttgt agacacagta ccggatgatg ttaagaggct gtatactgaa gcagcaacca
    49141 gtgactttgc tgcgttagcc caaacggctc atcgtcttaa aggcgtattt gccatgctaa
    49201 atctggtacc cggcaagcag ttatgtgaaa cgctggaaca tctgattcgt gagaaggatg
    49261 ttccaagaat agaaaaatac atcagcgaca ttgacagtta tgtcaagagc ttgctgtagc
    49321 aaggtagcct attacatgaa caatatgaac gtaattattg ccgatgacca tccgatagtc
    49381 ttgttcggta ttcgcaaatc acttgagcaa attgagtggg tgaatgttgt cggcgaattt
    49441 gaagactcta cagcactgat caacaacctg ccgaaactgg atgcgcatgt gttgattacc
    49501 gatctctcca tgcctggcga taagtacggc gatggcatta ccttaatcaa gtacatcaag
    49561 cgccatttcc caagcctgtc gatcattgtt ctgactatga acaacaaccc ggcgattctt
    49621 agtgcggtat tggatctgga tatcgaaggg atcgtgctga aacaaggtgc accgaccgat
    49681 ctgccgaaag ctctcgccgc gctgcagaaa gggaagaaat ttaccccgga aagcgtttct
    49741 cgcctgttgg aaaaaatcag tgctggtggt tacggtgaca agcgtctctc gccaaaagag
    49801 agtgaagttc tgcgcctgtt tgcggaaggc ctcctagtga ccgagatcgc taaaaagctg
    49861 aaccgcagta ttaaaaccat cagtagccag aagaaatctg cgatgatgaa gctgggcgtc
    49921 gagaacgata tcgccctgct gaattatctc tcttctgtga ccttaagtcc ggcagataaa
    49981 gactaatcat ctgtaggcca gataagacgc gttagtgtct tatctggcat ttgcaccgat
    50041 tgccggatgc ggcgtaaacg ccttatccgg cctacgattc ccattatttc aacagattac
    50101 attaaagtag gccagataag acgcgtcagt gtcttatctg gcatttgcac tgaatgccgg
    50161 atgcggcgta aacgccttat ccgtcctacg aatcccgcga tttcctgacc ctctcggcat
    50221 ataccgtcag cgtctttttt atcacatcca gcgttaccgg cttcgagagg cagctgtcca
    50281 taccggactc cagacaccgc tgcttctctt cagccaacgc attagcagtt actccgatta
    50341 ccggcaacgt cagtcccaac tgacgaatgc gttgcgtcaa gcggtaacca tccatatttg
    50401 gcatgttgac gtcgctaagc acgatatcaa tatgattctt actaagtaca ttaagcgcat
    50461 cgacgccatc attcgcggtt ttacattgat agcccaacga tcccaactga tctgccagca
    50521 gacgtcggtt aatcggatga tcatccacga ccagaatcat catatcgtcg ttatcgctga
    50581 ccgctttgtc cgtcgacggc agagcgttag caggatcgtc gctctccatc tcgatcaaat
    50641 aaatacgcgc caacaatgcc ggtagctcat gcggagcagc cacactgtgt acccactccc
    50701 ctggcgcttt ctccagcgga ataccaatat ggcgacgaca gaaggtcact accgctctgc
    50761 cctgccattt tttactcact acctcgtcag tgattaacac atcttcggga gtcggttcct
    50821 gcccttcgta tgttgtaacg acgatgccgc tgcgctgcaa actggtttcc aggaactgac
    50881 agagcgacgc attgcggacc gccagccagc agcgtttacc actcaaccct tccacgcctt
    50941 ttttctgcgg gtactgagcg ccgtacaacg gaatacgcac ggtaaactgg ctgcccattc
    51001 ccggttctga atctaccgag atatcgccgt ccatcatgct gatcagtttt tcacaaatcg
    51061 ccagacccag accggtcccc tggaagtttc gctgtacgcc cgttccaacc tggaagaagg
    51121 gatcaaacaa gcgcaccact tctttcgccg gaatccccac gccggtatcg cgaacacgga
    51181 tagagagata atcgccatca gcgcgaacat gcaaaactat acagccggta tcggtgaatt
    51241 ttatggcgtt actcaacagg ttggagatga cctgctgtaa acgcatcggg tcgccattta
    51301 aggccactgg cacatccggt tcaataaagc agtacaagcc taactgcttg cgtaccacca
    51361 gcggtaaata gttggcggtg atgtggttca tcacttcacg cggtgaaaac tcacgcggtt
    51421 cgatcttcaa ctgttccgat tcaatcttcg agaaatcgag aatatcgctg ataattttca
    51481 acaacaggct ggaagagttg ttcattgccg tcaccagacg atcgacgcct ttcggtaact
    51541 ctttggtttg caacagatcc aggttaccga taatgccata cagcggcgtt cgcagctcat
    51601 gactgacggt ggcaaggaac atcgattttg actggctcgc ctgttccgct gcttgtgcca
    51661 tctcctgcaa cgactcttcc atcttcacgc gcgaagaaac atccaccagc acacaaatgg
    51721 ccacgttttc attacgatag cgcgaatgga cgaagctgat ttgcagattg gtattgttgc
    51781 tggtcaggac atcaacaaaa ttgacctgct gcccacagat aatttgcgtc agtcgttggc
    51841 ggtcctcatg cgtaagcata ttgagatagg tatgcgccag ttcgttactt aaaatattga
    51901 cgccatcagc ggtacgcaaa atgcagatac ccactggcgc ggaggcgaca atcttgcgat
    51961 tgaactgctc atgttcttcc agtcgcaggg cgtcgctttc cgccggaatg aaaatacgtc
    52021 gctcgtacat ccgcgcgaga gtaaacaatg cagctccggc aagcacattc agcaaaattg
    52081 cgttaaggat caacatgcga atgcgttcca gcaccttatc aaccggcacc gaatacacga
    52141 tgcttagcga tgagggtggc agatttttct tcagcaccag ctcccggaac ccttccgtat
    52201 agccaaacca ggagcgttcc tgcatccagc gaggatcgcc cttaatttta ctttctggtc
    52261 cggtaagcga aatcagggta tgaccatttt catcaagaat ggtaaccccc atcggcaacg
    52321 tacccggtaa gaaaaagttc tccatccgga tggtctgctc gacacccaaa agcgcctgca
    52381 accggttcgc cagataaact ggcgtcaacg cgtaaaaata cccgacgcca gggcgcggac
    52441 cttcgctgat ccagtagagg ttactgccgc tatcatcttg tggtgcattt cgatatttat
    52501 tgatgcgttc atgcaacgct ttcaacgcgg tatcgcgttc cactggcata tcacgcagac
    52561 cgaaattggc catgcagagg ttatcactgc cgattaaaaa tacccggttg agatcgtaag
    52621 ccgcagaaaa attatcgcgc cagtagcgca taaaccacgc caatgactcc agagaacctc
    52681 gccaggtgtt actcattgcg gaacaatcgg agtcggcaaa cagcggttca aacgcaggca
    52741 catccgcctg cgtttctcgt ccacgcgggg aaagcacacc gttttctgcc gataagcgat
    52801 tttcggcgat gtacttaagc tctttcatca catcagaggt gcgttgaata aagcgctgag
    52861 cctgatcgga actcagatta aattcctgac gaatttccga ttctcgctga tgtaacgcat
    52921 taacgatgta aaaaacggat gaaaaagcaa tcaacagcca gagcactaac gccaatgctc
    52981 tgaacatgta gcgcgaggct ttcagggttg tacgaaaaga agcaaggtat ttcaaagggg
    53041 cgaagctccg cctcaggtga ccgatggagt gtggttaagg tagcggtaaa agcgtgttac
    53101 cgcaatgttc tgtcaccatt gcaataatgc gtatcgctta gaaaccattg ccttaaccag
    53161 gaaagccgga gtagatggac agttctcagt acgaaaaata agaaatgacc aatctgccga
    53221 cgcttgttaa tggttatatc ctggcgtcgg cagatcgccc agcagtctta tctgaaaaaa
    53281 gagagagcca ggttgtgtga agggatggat caccagtcaa aacttttcac cagcgtcagc
    53341 tcgccagcat tacgcatcgg tacaataaat gtttcctgtt tctcattgac cgatccttca
    53401 tcggtgatca gcgtcagttg ggcggtcgtc aattccgttt cgctgcgccc accatagtag
    53461 ttgatataca cctgatagcg cccgtgaact ggtgctggca tggcgaaaat ctcgggtccg
    53521 taccccgtcg tgacatccat atccagtgca ccactgtttt tcagcacggt gttaccgtac
    53581 caggcgtgtt cgccatcggg cgtgacaaca tggagatcga gatcggtatt gtccatatcc
    53641 cacgagagaa ccagccgtaa acgtgcacga atagttcctg tacccggcgt tgagtaaaac
    53701 tgcatttttt gccggctttg cccatccggg ctgatgacct gcacgctgtt gctgccttca
    53761 gtgaaaatat aagggcgtgc aaatgaacca tcggattcaa ttctctgcgg catactggca
    53821 ccgttgacca ccagccggcc ctgctgaact ttacccgccg cagcataatt ctttattttg
    53881 ccgctgatct gcgctgagat attttgatca tctgccatgt tgacggacga cgccgggtaa
    53941 ttgatcgatt gtgaaaaact ggcatcttcg ccctctgccg gatgccagcc agagagcggt
    54001 gcgtcaatct cgacttcctg cacaccttca ctgtgagcga caggcgaaag tgccaccagt
    54061 aacaacggaa gaaaaatctt tcgcatagca ttagtccatt aataattgtc gggtcagcgt
    54121 ttcgatatag ttctcatcaa gcccggtggg ataaccatca aaggccaagt gcagatactc
    54181 atgggttaaa tccagccgat cctgaagcgt aaagaagtta cgaataaaca gccgtttctg
    54241 ctgccgatcg gtatagggga aacctgagac taaacgacag accgcaaaca cgtctggttc
    54301 gttgtacccc gtctcgcctt gtaatatacg ccgccactgc ggcatttttt tcgccagcca
    54361 ggctttcgct ttgggtaata actggcaggt tgagcgtgga gctccccagc ggctaaggct
    54421 gttgtcggga taagcaaacg cgagtatctg gtcgtaacgc tccccctgcc ccgcctgggc
    54481 cgtggcttgc cgccaggaaa gtgtcccttc ggtagcgcga ctgccatgat aatgaaccgg
    54541 atcgcctgca tagatgagct cctgcgtcca ggcggtcatc gttcgcgccc ccgtggtcgc
    54601 tggcgaagca gaaacacgct gcgtggcgct gctgccgggg atggtcaggc aatcaccttc
    54661 ccgattggcg ttttgctgta aaaaagtacg tatcgccaca gtcatggctt tcgctgcctc
    54721 aggtggcgta cttttcgcct cgcgatccag aactctggca acgtactctt cacgatcgag
    54781 atgcgactgc aatttcagtt tgcccttttc gctcagtaag gtcgtctcac cgtggctgac
    54841 aaacgtaatg tggttaccat tagtaaacgt cacacgatag cgcccattaa gtacgctggg
    54901 ttttactgcc gtcgtactct tttccgctgt gattttcttc agcggatagc gggcaaacag
    54961 ctccacctct acacattgcc cactggcaac ctgcgtcggc actggcaaaa cccgattaag
    55021 caccgtcgca taacgggtta gtaccgtttt gctggtgcca ctgccagtaa cccacagcgg
    55081 tgtaccatcg gttaaccagc cagcaaaacc accctgacgg atttcctgtt tatcatcggc
    55141 aaaccagctc caggttttca cccgtacccg actaccgagc atggatgaga caccgatttt
    55201 cgcctcgtcc agcaccacat ccagtaagac ttcctgagct ttgttttgcg caggtaacgt
    55261 cgctaatgaa tcaagcagcg atttaaccgt taccgaggtt tctggcttca gcgttgtaag
    55321 agacgctaac cacgcaggtg cctgacgctg ttgccagtat tgcccccaca catctgcgcc
    55381 aatatgcaac cgctgcggcg caaaatagag cccacaggaa cgtaccagcg cggtatcgcg
    55441 cgttatgctc tccccagcct gacaacaata aacctcttcc ggggagtttc cgcgacactg
    55501 gtagggctgc tccggttgat gggtatcctc cagccaggcg taaacataca gtttccacaa
    55561 actccctaac ggcgtggtga gcgagtcagg taatgctgag actttggtca cgcctgatga
    55621 cgacagctga tacagttgat cgtgctgcgc accgcgcagc accagttgta acggcgtctc
    55681 ctccgcaagc gttggcagag taaccaacgc caatagccag acgattctgc gccagttcac
    55741 ttatttcacc tgcatcccgg tccattcact ccctgccgcg acactttgct gcgcaggtgc
    55801 ataggaacgc acataacgag caggaggcag aacgaattgc cctttttgcg agaagcgcag
    55861 caaatggcgg aaagtgaccg ttcccgtcag ttctttcacc ggcaccatat aggccagttc
    55921 gcccatttca ttacgcgctt tttccagcaa ttgcccctgc tgttccgcgg cgttgggttt
    55981 attgactgaa atgccccatg ttgtgcgctc aacgtcggct cccggcggga gcggtacttc
    56041 cacctgacca tagcgcagaa ctgcatcctg ctcgcaggta agcgtaattt catcgagata
    56101 cagcgcatcg ctgtcaatct cattgctggt caccggctgc agagtaaagc tcatctcttc
    56161 ttcaccgggg ataagccgat acaactggcg ttcaacggtc accggaatgt tactttgttg
    56221 agccgttttt gccggttcac gccagcggac ctgcacattt tgcggcgata attcgtcacc
    56281 aaaagagaga atgtccggca caccctgacc aacccaacgc cagtcttcgc cccctccagt
    56341 taacttatgt ttagcccatg cgcccgcagg cgcaggcaaa acaactggag gcattgtcgc
    56401 catatatttc gccagccagt tcatggccag cgcgcgctca atagtggatt gctcagcggt
    56461 caaaccatta aaatagcggc cgtctgggta gcgtcaccac cgctattgag cagcacaacc
    56521 gtgtgcgcca gcggctgatt gctgtttgcc gctttatccc acgcatattg ctgaacctgt
    56581 ggcatcaccg tggacttcaa ctgcgctttt ttcagcaacg tcatggttaa gacgtttgcc
    56641 accgcatctg ccagtggaga ttccggtgta tcgaggatca ggctgtcatt gatatcgcgg
    56701 atgtcttcct cagagaaatc ttcatcttta gttccgcgcc gggcgatagc ttcatccccc
    56761 cctttcaaca acgttttgca cggcagattc atctcctgtg cccacgccag caccagcgcc
    56821 cgatgcaata acggcatgtt atccgcctgc tcggcataac tgtcgagcat atgctgccag
    56881 tattccggtt gttgcgttac gccgagcgcc tggctggcct gccaatcggc gtaccatgcc
    56941 catgccgtaa ggaaggcgtc accattgcca tcttcacccc accaggtaaa gcgcgctccg
    57001 ggccccgcca gttgcatcag ccgcagacgg ttatcctgaa tcatctgacg aatgtcgtta
    57061 gcggcggcac tttgatgatc ggcaagcgaa cgccaggcga gactgagcgg gatcagacgg
    57121 ctaccggtgt tgatcacccc accccacggt tcatcgacta acgcatcaag attgttgcgg
    57181 aaaatctcct gcggcgtttc actactttgt agccggatat tactcgcctg ctcgggcaac
    57241 atcagcgcgt tctcgccacc gccgagcatg acatttttct gctgttcaac gggccagctg
    57301 ttgtccacaa aagacagttt tgtgctaatg ctgtcctgca cttgcccatt ttgttgcagt
    57361 tctgcactta acaagccaga ttgctgaata ttctgcgtca gcgaaatata attcgcccct
    57421 ttgtgcagcg tcagcgtctg gcgcatctca gcgcctgcaa atttagtcac cagcgccacc
    57481 ggttcgttat cctgctgact gaagataaac agtcctgccg ccggtttgtc gcccacgcga
    57541 tacaccgttg gcatactcca cttcatgtag agattttttt ccgaacgcag ataagcacgc
    57601 ccctgcccga ccagcccgtc gccgttcatc ccacgcgcgg tgatacgcca gcgggttaac
    57661 gaatcaggca tcaggaacgt gaaatacgct ttgccttgtt tatcggttgt gagtgacggc
    57721 atccatgccg cggtatccac ctcttcacgc cgtggacgtt caggcatttt tactcgccgc
    57781 tcgctgcggt tagtcgcgcc aggcgcaacc ggctcgctgg agagcgcctg gtcatagctg
    57841 ataaacgaca gactggagct ggtacgcaca ttgttacgcc ccagcggata gaaaaatttg
    57901 ccgatattcg gcgcgatttc tggttgcagc gcgtagatca tttcatcgac cacgcctacc
    57961 gttagctgcg cagaaacagg tttacctttc agcgacgagg ttaat
//
LOCUS       DADMGC010000009        56573 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.009, whole genome shotgun sequence.
ACCESSION   DADMGC010000009 DADMGC010000000
VERSION     DADMGC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.009"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..1017)
                     /gene="setA"
                     /locus_tag="JNZ73_000564"
     CDS             complement(<1..1017)
                     /gene="setA"
                     /locus_tag="JNZ73_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HAY8730960.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGA"
     gene            complement(1119..1250)
                     /gene="sgrT"
                     /locus_tag="JNZ73_000565"
     CDS             complement(1119..1250)
                     /gene="sgrT"
                     /locus_tag="JNZ73_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HAY8730961.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1339..2994
                     /locus_tag="JNZ73_000566"
     CDS             1339..2994
                     /locus_tag="JNZ73_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HAY8730962.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGDLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3028..3127
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3158..4141
                     /gene="thiB"
                     /locus_tag="JNZ73_000567"
     CDS             3158..4141
                     /gene="thiB"
                     /locus_tag="JNZ73_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HAY8730963.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPSGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4117..5727
                     /gene="thiP"
                     /locus_tag="JNZ73_000568"
     CDS             4117..5727
                     /gene="thiP"
                     /locus_tag="JNZ73_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HAY8730964.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5711..6409
                     /gene="thiQ"
                     /locus_tag="JNZ73_000569"
     CDS             5711..6409
                     /gene="thiQ"
                     /locus_tag="JNZ73_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HAY8730965.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(6523..7287)
                     /locus_tag="JNZ73_000570"
     CDS             complement(6523..7287)
                     /locus_tag="JNZ73_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8730966.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7373..8251)
                     /gene="araC"
                     /locus_tag="JNZ73_000571"
     CDS             complement(7373..8251)
                     /gene="araC"
                     /locus_tag="JNZ73_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HAY8730967.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8590..10226
                     /gene="araB"
                     /locus_tag="JNZ73_000572"
                     /pseudo
     CDS             8590..10226
                     /gene="araB"
                     /locus_tag="JNZ73_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
     gene            10237..11739
                     /gene="araA"
                     /locus_tag="JNZ73_000573"
     CDS             10237..11739
                     /gene="araA"
                     /locus_tag="JNZ73_000573"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAY8730968.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            11939..12634
                     /gene="araD"
                     /locus_tag="JNZ73_000574"
     CDS             11939..12634
                     /gene="araD"
                     /locus_tag="JNZ73_000574"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY8730969.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12709..15060
                     /gene="polB"
                     /locus_tag="JNZ73_000575"
     CDS             12709..15060
                     /gene="polB"
                     /locus_tag="JNZ73_000575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HAY8730970.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHNPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWVRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15224..18130
                     /gene="rapA"
                     /locus_tag="JNZ73_000576"
     CDS             15224..18130
                     /gene="rapA"
                     /locus_tag="JNZ73_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HAY8730971.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAILLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNTVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18142..18801
                     /gene="rluA"
                     /locus_tag="JNZ73_000577"
     CDS             18142..18801
                     /gene="rluA"
                     /locus_tag="JNZ73_000577"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HAY8730972.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARAVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(18918..19733)
                     /gene="djlA"
                     /locus_tag="JNZ73_000578"
     CDS             complement(18918..19733)
                     /gene="djlA"
                     /locus_tag="JNZ73_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HAY8730973.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            19988..22342
                     /gene="lptD"
                     /locus_tag="JNZ73_000579"
     CDS             19988..22342
                     /gene="lptD"
                     /locus_tag="JNZ73_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HAY8730974.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22395..23681
                     /gene="surA"
                     /locus_tag="JNZ73_000580"
     CDS             22395..23681
                     /gene="surA"
                     /locus_tag="JNZ73_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HAY8730975.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRANAYVKILSN"
     gene            23681..24670
                     /gene="pdxA"
                     /locus_tag="JNZ73_000581"
     CDS             23681..24670
                     /gene="pdxA"
                     /locus_tag="JNZ73_000581"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAY8730976.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24667..25488
                     /gene="rsmA"
                     /locus_tag="JNZ73_000582"
     CDS             24667..25488
                     /gene="rsmA"
                     /locus_tag="JNZ73_000582"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAY8730977.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25491..25868
                     /gene="apaG"
                     /locus_tag="JNZ73_000583"
     CDS             25491..25868
                     /gene="apaG"
                     /locus_tag="JNZ73_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HAY8730978.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25875..26717
                     /gene="apaH"
                     /locus_tag="JNZ73_000584"
     CDS             25875..26717
                     /gene="apaH"
                     /locus_tag="JNZ73_000584"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HAY8730979.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26795..27274)
                     /gene="folA"
                     /locus_tag="JNZ73_000585"
     CDS             complement(26795..27274)
                     /gene="folA"
                     /locus_tag="JNZ73_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HAY8730980.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27465..29327)
                     /gene="kefC"
                     /locus_tag="JNZ73_000586"
     CDS             complement(27465..29327)
                     /gene="kefC"
                     /locus_tag="JNZ73_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HAY8730981.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29320..29850)
                     /gene="kefF"
                     /locus_tag="JNZ73_000587"
     CDS             complement(29320..29850)
                     /gene="kefF"
                     /locus_tag="JNZ73_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HAY8730982.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(29898..31292)
                     /locus_tag="JNZ73_000588"
     CDS             complement(29898..31292)
                     /locus_tag="JNZ73_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8730983.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(31351..31638)
                     /gene="fixX"
                     /locus_tag="JNZ73_000589"
     CDS             complement(31351..31638)
                     /gene="fixX"
                     /locus_tag="JNZ73_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HAY8730984.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31635..32921)
                     /locus_tag="JNZ73_000590"
     CDS             complement(31635..32921)
                     /locus_tag="JNZ73_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY8730985.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSLSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESSPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(32972..33913)
                     /locus_tag="JNZ73_000591"
     CDS             complement(32972..33913)
                     /locus_tag="JNZ73_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAY8730986.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(33928..34698)
                     /locus_tag="JNZ73_000592"
     CDS             complement(33928..34698)
                     /locus_tag="JNZ73_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HAY8730987.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVNA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35169..36683
                     /gene="caiT"
                     /locus_tag="JNZ73_000593"
     CDS             35169..36683
                     /gene="caiT"
                     /locus_tag="JNZ73_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HAY8730988.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFDIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36714..37856
                     /gene="caiA"
                     /locus_tag="JNZ73_000594"
     CDS             36714..37856
                     /gene="caiA"
                     /locus_tag="JNZ73_000594"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HAY8730989.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            37985..39202
                     /gene="caiB"
                     /locus_tag="JNZ73_000595"
     CDS             37985..39202
                     /gene="caiB"
                     /locus_tag="JNZ73_000595"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HAY8730990.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            39276..40829
                     /gene="caiC"
                     /locus_tag="JNZ73_000596"
     CDS             39276..40829
                     /gene="caiC"
                     /locus_tag="JNZ73_000596"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HAY8730991.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            40938..41723
                     /gene="caiD"
                     /locus_tag="JNZ73_000597"
     CDS             40938..41723
                     /gene="caiD"
                     /locus_tag="JNZ73_000597"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HAY8730992.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            41729..42319
                     /gene="caiE"
                     /locus_tag="JNZ73_000598"
     CDS             41729..42319
                     /gene="caiE"
                     /locus_tag="JNZ73_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HAY8730993.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(42405..42800)
                     /gene="caiF"
                     /locus_tag="JNZ73_000599"
     CDS             complement(42405..42800)
                     /gene="caiF"
                     /locus_tag="JNZ73_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HAY8730994.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(43062..46283)
                     /gene="carB"
                     /locus_tag="JNZ73_000600"
     CDS             complement(43062..46283)
                     /gene="carB"
                     /locus_tag="JNZ73_000600"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAY8730995.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(46301..47449)
                     /gene="carA"
                     /locus_tag="JNZ73_000601"
     CDS             complement(46301..47449)
                     /gene="carA"
                     /locus_tag="JNZ73_000601"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAY8730996.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(47904..48725)
                     /gene="dapB"
                     /locus_tag="JNZ73_000602"
     CDS             complement(47904..48725)
                     /gene="dapB"
                     /locus_tag="JNZ73_000602"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAY8730997.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(48892..49806)
                     /gene="rihC"
                     /locus_tag="JNZ73_000603"
     CDS             complement(48892..49806)
                     /gene="rihC"
                     /locus_tag="JNZ73_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HAY8730998.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(49872..50822)
                     /gene="ispH"
                     /locus_tag="JNZ73_000604"
                     /gene_synonym="lytB"
     CDS             complement(49872..50822)
                     /gene="ispH"
                     /locus_tag="JNZ73_000604"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAY8730999.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(50824..51273)
                     /gene="fkpB"
                     /locus_tag="JNZ73_000605"
     CDS             complement(50824..51273)
                     /gene="fkpB"
                     /locus_tag="JNZ73_000605"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY8731000.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(51360..51854)
                     /gene="lspA"
                     /locus_tag="JNZ73_000606"
     CDS             complement(51360..51854)
                     /gene="lspA"
                     /locus_tag="JNZ73_000606"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAY8731001.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(51854..54670)
                     /gene="ileS"
                     /locus_tag="JNZ73_000607"
     CDS             complement(51854..54670)
                     /gene="ileS"
                     /locus_tag="JNZ73_000607"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAY8731002.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAAILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(54713..55654)
                     /gene="ribF"
                     /locus_tag="JNZ73_000608"
     CDS             complement(54713..55654)
                     /gene="ribF"
                     /locus_tag="JNZ73_000608"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAY8731003.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(55662..55880)
                     /locus_tag="JNZ73_000609"
     CDS             complement(55662..55880)
                     /locus_tag="JNZ73_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HAY8731004.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            55983..56246
                     /gene="rpsT"
                     /locus_tag="JNZ73_000610"
     CDS             55983..56246
                     /gene="rpsT"
                     /locus_tag="JNZ73_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAY8731005.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        13545 a        15496 c        14681 g        12851 t
ORIGIN      
        1 tgcccccgct cttccaggca ttaaatcctg aaaccatagc atcccaatac ccgcaacaat
       61 gccgataaat acagcgttaa aaagttgcag cgtcatcaac gccatacggc tatgaaagaa
      121 aatcaatcca gtgtaaaaca gcactcctgc caccactgca atgaccatca ttcgccgctt
      181 accataacgt ttgacatagt agccagccag aatcattgct ggtatttcca gtccagctgc
      241 cgtccccatc aggaaacccg ccagtttgtc tggcaatcct aaatcgctac tgatccacaa
      301 cggcatatca ataatgtaca tagtgttgca ggtccacatt aacgtcgagg cgacaaataa
      361 catccgtacg ttactatcct gccagtcacc ttgcattgat aaagcatttt ccgacggcag
      421 ttctacccgc gccacagacg gaagcataaa tgcaatcaat accagactga gtgtgaatat
      481 cccggcggca atcgaaaaca tcactgtaaa gccgtaattc aacgccagca taaaggccag
      541 cggtggaccg attacccatg ccagagaaag ctgcgcacgc atcaccgagc taaacatcac
      601 cacttctcgc gccgagttat ccgcgtattc ccgcgccaga gcaaataact gtggcattgc
      661 cgtattggcc agagatgcca gaagcacacc acaggtgata agcgtcagat aatgacgatt
      721 aaatgcaaac agtagcgcat tgccaatagc catcaaacag caaaatataa tcagttttcg
      781 ccgatcgccc tgactgtcag aacgttttgc caaccagagg cttacgccga tcccagcaat
      841 agcattcacc gtataaaaga gtccgatcca gaaaggttgc gcgccaacct cacgactcag
      901 aaataagctc aatgtaggag cctgtagcgc cccggccacc cccatcataa aagcgaccag
      961 cataaatgcc gcgtaaacac cgttcatacg gcgagccatc gtcattatcc agatcatacg
     1021 ttcccttttt agcgcggcga gaataaaaaa aaccagcagg tataatctgc tggcgggtga
     1081 ttttacacca atactcagtc acacatgatg caggcaagtc aactttcaga attgcggtca
     1141 tcccactgca tcagtccttc aagcatggtt aatcgttgtg ggacgcttaa ccaacgcaac
     1201 cagcacaact tcgctgtcgc ggtaaaatag tgctgataaa actgacgcat ggggcatctc
     1261 cttgcttcat cgttaggaat tattgactta atatagggaa aataaaattg ctgtcttttg
     1321 cgcaggagtt ccccttttat gccatctgct cgtctgcaac aacagttcat ccgcctgtgg
     1381 caatgctgcg agggtaaatc gcaggacaca acgctcaacg aactggcagc gttattgagc
     1441 tgctcgcgtc gtcatatgcg taccctgctc aacaccatgc aggatcgcgg ctggctgacg
     1501 tgggaagcgg aagtcgggcg cggtaaacgc tcgcgtctga cattcctcta taccgggctg
     1561 gcgcttcagc aacagcgggc ggaagacctg ctggagcagg atcgtatcga ccaactagtg
     1621 cagttggttg gcgacaaagc gactgtgcgg caaatgctgg tttctcatct gggccgcagc
     1681 ttccgccagg ggcggcacat cctgcgcgtg ctctactatc gtccgttgcg taatctgcta
     1741 cctggcagcg cattgcgccg ttccgaaacc catattgccc ggcaaatctt cagttcgcta
     1801 acgcgcataa atgaggaaaa tggggaactg gaagcagaca tcgcccacca ctggcagcaa
     1861 atatcaccgc ttcactggcg tttctttttg cgtccaggag tccattttca tcatggtcgt
     1921 gaactggaaa tggacgacgt gatcgcctct ttaaaacgaa tcaatacgct gccgctctat
     1981 tcgcatatta cggacattgt gtcgccgacg ccctggacgc tggatatcca tctcacgcag
     2041 ccggaccgct ggttgccgtt actgctgggg caagttccgg cgatgatcct gccgtgcgaa
     2101 tgggaaaccc tcagtaactt tgccagccat cctattggca ccggtccgta tgcggtgatt
     2161 cgcaacagca ccaatcaact gaaaattcag gcattcgatg acttcttcgg ttaccgggca
     2221 ttaatcgacg aagttaacgt ctgggttctg ccggaaattg ccgacgagcc agccggtgat
     2281 ctgatgctaa aaggtccaca gggcgaggaa aaagagattg aaagccgcct ggaggaaggt
     2341 tgctactatt tactgttcga cagccgcacc catcgcgggg cgaatcagca agtcagggac
     2401 tgggtaagct atgtgctttc tccaactaat ctggtctatt tcgctgagga acagtaccag
     2461 caactgtggt tcccggctta tggactgctc ccccgttggc accatgcccg caccataaag
     2521 agcgaaaaac cggctggcct ggaaagcctc accctgacct tttatcagga tcacagtgag
     2581 catcgggtga ttgccgggat catgcagcag attctggcaa gtcaccaggt cacgctggaa
     2641 atcaaagaga tcagctacga tcagtggcat gaaggagaga tcgagagcga tatctggctt
     2701 aatagcgcca actttacgct gccgctggat ttttcgctgt tcgcgcactt gtgcgaggtg
     2761 ccgctgctcc aacactgtat tccgatcgac tggcaagccg acgccgcccg ctggcgcaat
     2821 ggcgaaatga acctggcgaa ctggtgccag caactggtcg ccagtaaagc aatggtgccg
     2881 cttatccacc actggctgat cattcaggga caacgcagta tgcgcggcct gcgtatgaat
     2941 accctcggct ggttcgattt taaatcagcg tggtttgcgc caccggatcc atgattgctg
     3001 gtagataaca aaatcactac actaacgccg ttctcaacgg ggtgccacgc aaacgcgtgc
     3061 gctgagaaaa tacccgtcga acctgatccg gataacgccg gcgaagggat ttgaggctcc
     3121 ttctcaagtc ctttgccact cttttttgag gtgcaaagtg ttaaaaaaat gtcttcccct
     3181 gctgttgctg tgcacagcgc ccgttttcgc taaacccgtt ctgactgttt atacctacga
     3241 ttccttcgcc gccgactggg ggcctggtcc aaagatcaaa aaagcttttg aagccgactg
     3301 taactgcgaa ctgaaactgg tggcgctgga agatggcgtt tcgcttctca accgtctacg
     3361 gatggaaggc aaaaatagta aagccgatgt ggtgctgggg ctggataaca atctgttaga
     3421 cgccgccagt aaaaccggac tatttgccaa aagcggtgtg gcagcggatg ccgttaacgt
     3481 tcccagcggc tggaataatg acactttcgt cccgtttgat tacggctatt tcgccttcgt
     3541 ctatgacaag aacaaactga aaaacccgcc gcaaagcctg aaagaattgg ttgagagcga
     3601 tcagaactgg cgggtgattt atcaggatcc gcgtaccagc acgccggggc tgggtctgct
     3661 gttgtggatg caaaaagtct atggcgatga cgccccacaa gcctggcaaa aactggcgaa
     3721 gaaaaccgtc acggtcacca aaggctggag cgaagcctac ggcctgtttt taaaaggtga
     3781 aagcgatctg gtactgagtt acaccacctc tccggcttat cacattctcg aagagaaaaa
     3841 agataactac gccgccgcga actttagcga aggtcactat ctgcaggtgg aagtcgccgc
     3901 ccgcaccgct gccagcaagc agccggagct ggcgcaaaaa ttcctccagt ttatggtttc
     3961 tccggctttt cagaatgcga tcccaaccgg taactggatg tatccggtgg caaatgtcac
     4021 gctgcctgcc gggtttgaac aattgaccaa accggccacc acgctggagt tcacaccagc
     4081 tgaagtggcg gcacaacgtc aggcatggat tagcgaatgg caacgcgccg tcagccgtta
     4141 attcccggct ggttaattcc aggtgtaagc gccgccacgc tggtggtagc ggttgcgctc
     4201 gcggcgtttc tcgccctgtg gtggaacgcg ccgcagggta actgggtggc agtctggcag
     4261 gacagctacc tgtggcatgt ggtgcgcttc tccttctggc aggcgtttct ctcggcgcta
     4321 ctctctgtcg tacccgcgat attcctcgcc cgcgccctct atcgcaggcg ctttccgggt
     4381 cggctggcgc tgttgcgtct gtgcgcaatg accttgatcc tcccggtgct agttgctgtt
     4441 ttcggcattc ttagcgtcta tggtcgtcag ggctggctgg catcgctctg ccaatcgctc
     4501 ggtctggagt ggaccttttc gccctacggc ctgcaaggca ttttgctggc gcacgtattt
     4561 tttaatctgc cgatggcgag ccgcttatta ctccaggcac tggaaaacat tcccggcgaa
     4621 caacgtcagc ttgccgccca gcttgggatg cgcggctggc attttttccg cttcgtcgaa
     4681 tggccgtggc tacggcgaca aatcccgccg gttgccgcgc ttatctttat gctctgtttc
     4741 gccagcttcg ccaccgtgct atcgctgggc ggcggtccgc aggcaaccac tatcgagctg
     4801 gctatttatc aggcgctgag ttacgactac gatcctgccc gcgcggcgat gctggcgctg
     4861 ctccagatgg tgtgttgcct tgggctggtg ctgttgagtc agcgactaag taaggccatt
     4921 gcgcccggca ccacgctgct gcaaggctgg cgcgatccgg acgatcgtct gcatagccgc
     4981 atttgcgaca cggtgttaat agtgctggcg ctgctgctgt tgctgccacc gttgctggcg
     5041 gtgatcgtcg atggggtaaa tcgccagttg ccggaagtgc tggcacaacc ggtgctgtgg
     5101 caggcgctgt ggacctcgtt gcgtattgcg ctggcggcag gtgtattgtg cgtagtgctg
     5161 accatgatgc tgctatggag cagtcgcgaa ctgcgggcgc ggcagaaaat gctggcgggt
     5221 caggcgctgg agatgagcgg catgttgatc ctcgccatgc cggggattgt gctggctacc
     5281 ggcttctttt tactgctcaa caacactatc ggcctgccac aatctgctga cggcattgtg
     5341 attttcacca atgcgttaat ggcgatccct tatgcgctga aagtactgga aaacccgatg
     5401 cgcgatatca ccgcccgcta cagtatgttg tgtcagtcgc tgggcattga aggctggtcg
     5461 cgcttaaaag tggtcgagct gcgcgccctg aaacgtccac tggcgcaggc gctggctttt
     5521 gcctgcgtgc tgtcgattgg tgattttggc gtagtggcgt tgttcggtaa cgatgatttc
     5581 cgtaccctgc cgttttatct ctaccagcaa attggctcct atcgcagcca ggacggcgcg
     5641 gtcaccgcgt taattctgct actactctgt tttctgctgt ttaccgtgat tgaaaaacta
     5701 ccggggcgaa atgttaaaac tgactgatat cacctggctt tatcaccatt tgccgatgcg
     5761 ttttagctta acggtagaac gcggcgagca ggtggcaatc ctcgggccaa gcggcgcggg
     5821 taaaagtacc ctgctgaatt tgattgccgg ttttctgacg ccagccagcg gttcgctgac
     5881 tatcgatggc gttgatcaca ccacgacgcc gccgtcacgc cgtccggtgt cgatgctgtt
     5941 tcaggagaac aacctgttca gccacctgac ggtcgcgcag aacatcgggc tggggctaaa
     6001 tccggggttg aaactgaacg cggcacagca ggagaaaatg cacgcgattg cccgtcagat
     6061 ggggattgat aatttaatgg cgcggttacc gggcgagctt tccggcggtc agcgacagcg
     6121 cgtggcgtta gcgcgttgtc tggtgcgcga acagccgatt ttattgctcg atgaaccgtt
     6181 ctctgcgctc gatccggcgt tacgtcagga gatgctgacg ctggtgagca cgagctgcca
     6241 gcagcaaaaa atgacgctat tgatggtgtc gcacagtgtg gaagatgcgg cgcggatcgc
     6301 cacacgctcg gtggtggtcg ccgacgggcg catcgcctgg cagggtaaga ctgaagaatt
     6361 gttgagcggt aaggcgagtg cttcggcaat attggggatt acgggttagt atgcactgtc
     6421 agatgcggcg tgaacacctt atccgaccta cggacagtgc gttttgtagg cctgataaga
     6481 cgcggtcagc gtcgcatgag gcaacccgca caagacaatc ccctaaaccc caaccacttt
     6541 acgcaaaata tcgatataca ccggcatcaa cgggtggcga attaacacca ccagcgccac
     6601 cacgccgatg gcagaaatca acggcgtcag ccacaacaaa cggccgcgcg acaaataatg
     6661 acttaaacga tcggttgctt taccgctgcg ccataaccgc cagcacagcc agccaccaac
     6721 ccacaaaaat accgccgtcg ccagcagcaa ccatttaaac tcgccgctct gcattccggc
     6781 tggaatatcg atcgccgcgc ccgccagaat cccaggcagg aagtaaaacg gtggccacag
     6841 caggcagccg ataatattcg gcgtaatgaa ttttgccacc ggcagatcca gcattcccgc
     6901 caccattggc accagcggac gcgtcgggcc aacaaagcga ccgaccagaa tggtgaacat
     6961 gctgtgttga tgcaacgcat gttcagtttt atcgagtaat gctttgtttt tcttcagaaa
     7021 tgaccagcga tgcaacggct ttttaaaacg ccagcccagc cagaaggaga tccagtcgcc
     7081 cagcagacaa ccgacaatcc ctgccagcca agcgtgccag aaacttaact cgccgctgcc
     7141 aatcagcgct cccagccccg ccatcagcac cgtaccgggt agaatcaaac cgaccagcgc
     7201 cagcgactcc agaaaggcca ccaacaccac cgccatcaat gaatacacgg tagattgggt
     7261 aataaagtgt tccagcaatg cttgcataat gtgcctgtca aatggacgaa gcagggattc
     7321 tgcaaaccct atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca
     7381 acttgacggc tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg
     7441 ccccggtgca ttttttaaat acccgcgaga aatagagttg atcgtcaaaa ccaacattgc
     7501 gaccgacggt ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc tggctgatac
     7561 gttggtcctc gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca
     7621 gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg
     7681 ccaggtgatc gctgatgtac tgacaagcct cgcgtacacg attatccatc ggtggatgga
     7741 gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca
     7801 gcagctccga atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc
     7861 tgaaatacgg ctggtgcgct tcatccgggc gaaagaaacc cgtattggca aatattgacg
     7921 gccagttaag ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc
     7981 attcgcgagc ctccggatga caaccgtagt gatgaatctc tcctggcggg aacagcaaaa
     8041 tatcacccgg tcggcagaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa
     8101 tggtgagatt gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat
     8161 aaccgttggc ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg
     8221 gcagcagggg atcattttgc gcttcagcca tacttttcat actcccgcca ttcagagaag
     8281 aaaccaattg tccatattgc atcagacatt gccgtcactg cgtcttttac tggctcttct
     8341 cgctaaccca accggtaacc ccgcttatta aaagcattct gtaacaaagc gggaccaaag
     8401 ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt
     8461 atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc
     8521 ctacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt ttttggatgg
     8581 agtgaaacga tggcgattgc aattggcctc gattttggca gtgattctgt gcgagctttg
     8641 gcggtggact gcgccagcgg tgaagagatc gccaccagcg tagagtggta tccccgttgg
     8701 caaaaagggc aattttgtga tgccccgaat aaccagttcc gtcatcatcc gcgtgactac
     8761 attgagtcaa tggaagcggc actgaaaacc gtgcttgcag agcttagcgt cgaacagcgc
     8821 gcagctgtgg tcgggattgg cgttgacagt accggctcga cgcccgcacc gattgatgcc
     8881 gacggtaacg tgctggcgct gcgcccggag tttgccgaaa acccgaacgc gatgttcgta
     8941 ttgtggaaag accacactgc ggttgaagaa gcggaagaga ttacccgttt gtgccacgcg
     9001 ccgggcaacg ttgactactc ccgctatatt ggcggtattt attccagcga atggttctgg
     9061 gcaaaaatcc tgcatgtgac tcgccaggac agcgccgtgg cgcaatctgc cgcatcgtgg
     9121 attgagctgt gcgactgggt gccagctctg ctttccggta ccacccgccc gcaggatatt
     9181 cgtcgcggac gttgcagcgc cgggcataaa tctctgtggc acgaaagctg gggcggcttg
     9241 ccgccagcca gtttctttga tgagctggac ccgatcctca atcgccattt gccttccccg
     9301 ctgttcactg acacctggac tgccgatatt ccggtgggca ccttatgccc ggaatgggcg
     9361 cagttggcgc aggcgcacag cctaacgcac tggtaaaagt tatcggtact tccacctgcg
     9421 acattctgat tgccgacaaa cagagcgttg gcgagcgggc agttaaaggt atttgcggtc
     9481 aggttgatgg cagcgtggtg cctggattta tcggtctgga agcaggccaa tcggcgtttg
     9541 gtgatatcta cgcctggttt ggtcgcgtac tcggctggcc gctggaacag cttgccgccc
     9601 agcatgcgga actgaaagcg caaatcaacg ccagccagaa acaactgctt ccggcgctga
     9661 ccgaagcatg ggccaaaaat ccgtctctgg atcacctgcc ggtggtgctc gactggttta
     9721 acggtcgtcg cacgccaaac gctaaccaac gcctgaaagg ggtgattacc gatcttaacc
     9781 tcgctaccga cactccgctg ctgttcggcg gtttgattgc tgccaccgcc tttggcgcac
     9841 gcgcaatcat ggagtgcttt accgatcagg ggatcgccgt caataacgtg atggcgctgg
     9901 gcggcatcgc gcggaaaaac caggtcatta tgcaggcctg ctgcgacgtg ctgaatcgcc
     9961 cgctgcaaat tgttgcctct gaccagtgct gtgcgctcgg tgcggcgatt tttgctgccg
    10021 tcgccgcgaa agtgcacgca gacatcccat cagcccagca aaaaatggcc agtgcggtag
    10081 agaaaaccct gcaaccgcgc agcgaacagg cacaacgctt tgaacagctt tatcgccgct
    10141 atcagcaatg ggcgatgagc gccgaacaac actatcttcc aacttccgcc ccggcacagg
    10201 ctgcccaggc cgttgcgact ctataaggac acgataatga cgatttttga taattatgaa
    10261 gtgtggtttg tcattggcag ccagcatctg tatggcccgg aaaccctgcg tcaggtcacc
    10321 caacatgccg agcacgttgt taatgcgctg aatacggaag cgaaactgcc ctgcaaactg
    10381 gtgttgaaac cgctgggcac cacgccggat gaaatcaccg ctatttgccg cgacgcgaat
    10441 tacgacgatc gttgcgctgg tctggtggtg tggctgcaca ccttctcccc ggccaaaatg
    10501 tggatcaacg gcctgaccat gctcaacaaa ccgttgctgc aattccacac ccagttcaac
    10561 gcggcgctgc cgtgggacag tatcgatatg gactttatga acctgaacca gactgcacat
    10621 ggcggtcgcg agttcggctt cattggcgcg cgtatgcgtc agcaacatgc cgtcgttacc
    10681 ggtcactggc aggataaaca agcccatgag cgtatcggct cctggatgcg tcaggcggtc
    10741 tctaaacagg atacccgtca tctgaaagtc tgccgatttg gcgataacat gcgtgaagtg
    10801 gcggtcaccg atggcgataa agttgccgca cagatcaagt tcggtttctc cgtcaatacc
    10861 tgggcggttg gcgatctggt gcaggtggtg aactccatca gcgacggcga tgttaacgcg
    10921 ctggtcgatg agtacgaaag ctgctacacc atgacgcctg ccacacaaat ccacggcgaa
    10981 aaacgacaga acgtgctgga agcggcgcgt attgagctgg ggatgaagcg tttcctggaa
    11041 caaggtggct tccacgcgtt caccaccacc tttgaagatt tgcacggtct gaaacagctt
    11101 ccaggtctgc ccgtacagcg tctgatgcag cagggttacg gctttgcggg cgaaggcgac
    11161 tggaaaaccg ccgccctgct tcgcatcatg aaggtgatgt caaccggtct gcagggcggc
    11221 acctccttta tggaggacta cacctatcac ttcgagaaag gtaatgacct ggtgctcggc
    11281 tcccatatgc tggaagtctg cccgtcgatt gccgtagaag agaaaccgat cctcgacgtt
    11341 cagcatctcg gtattggtgg taaggacgat cctgcccgac tgatcttcaa tacccaaacc
    11401 ggtccagcga ttgtcgccag cctgattgat ctcggcgatc gttaccgtct gctggttaac
    11461 tgtatcgaca cggtgaaaac accgcactcc ctgccgaaac tgccggtggc taatgcactg
    11521 tggaaagcgc aaccggatct gccaactgct tccgaagcgt ggatcctcgc tggtggcgcg
    11581 caccataccg tcttcagcca tgcgctgaac ctcaacgata tgcgccagtt cgccgagatg
    11641 cacgacattg aaatcacggt gattgataac gacacccgcc tgccagcgtt taaagacgca
    11701 ctgcgctgga acgaagtgta ttacggattt cgtcgctaag tagccacatc aggtatgtaa
    11761 cgcctgatgc gacgctgact cgtcttatct ggcctacacg ctgcgatttt gtaggccgga
    11821 taagcaaagc gcatccggca ttcaacgcct gatgcgacgc tggcgcgtct tatcaggcct
    11881 acgcgctgcg attttgtaga ccggataagc gaagcgcatc cggcacgaag gagtcaacat
    11941 gttagaagat ctcaaacgcc aggtattaga agccaacctg gcgctgccaa aacacaacct
    12001 ggtcacgctc acatggggca acgtcagcgc cgttgatcgc gagcgcggcg tctttgtgat
    12061 caaaccttcc ggcgtcgatt acagcgtcat gaccgctgac gatatggtcg tggttagcat
    12121 cgaaaccggt gaagtggttg aaggtacgaa aaagccctcc tccgacacgc caactcaccg
    12181 gctgctctat caggcattcc cctccattgg cggcattgtg catacgcact cgcgccacgc
    12241 caccatctgg gcgcaggcgg gtcagtcgat tccagcaacc ggcaccaccc acgccgacta
    12301 tttctacggc accattccct gcacccgcaa aatgaccgac gcagaaatca acggcgaata
    12361 tgagtgggaa accggtaacg tcatcgtaga aacctttgaa aaacagggta tcgatgcagc
    12421 gcaaatgccc ggcgttctgg tccattccca cggcccgttt gcatggggca aaaatgccga
    12481 agatgcggtg cataacgcca tcgtgctgga agaggtcgct tatatgggga tattctgccg
    12541 tcagttagcg ccgcagttac cggatatgca gcaaacgctg ctggataaac actatctgcg
    12601 taagcatggc gcgaaggcat attacgggca gtaatgactg tataaaacca cagccaatca
    12661 aacgaaacca ggctatactc aagcctggtt ttttgatgga ttttcagcgt ggcgcaggca
    12721 ggttttatct taacccgaca ctggcgggac accccgcaag ggacagaagt ctccttctgg
    12781 ctggcgacgg acaacgggcc gttgcaggtt acgcttgcac cgcaagagtc cgtggcgttt
    12841 attcacgccg atcaggttcc ccgcgctcag catattttgc agggtgaaca aggctttcgt
    12901 ctgacaccgc tggcgttaaa ggattttcac cgccagccgg tgtatggcct ttactgtcgc
    12961 gcccatcgcc aattgatgaa ttacgaaaag cgcctgcgtg aaggtggcgt taccgtctac
    13021 gaggccgatg tgcgcccgcc agaacgctat ctgatggagc ggtttatcac ctcaccggtg
    13081 tgggtcgagg gtgatatgca caatggcact atcgttaatg cccgtctgaa accgcatccc
    13141 gactatcgtc cgccgctcaa gtgggtttct atagatattg aaaccacccg ccacggtgag
    13201 ctgtactgca tcggcctgga aggctgcggg cagcgcatcg tttatatgct ggggccggag
    13261 aatggcgacg cctcctcgct tgatttcgaa ctggaatacg tcgccagccg cccgctgctg
    13321 ctggaaaaac tcaacgcctg gtttgcaaac cacaatcccg atgtgatcat cggctggaac
    13381 gtggtgcagt tcgatctgcg aatgctgcaa aaacatgccg agcgttaccg tcttccgctg
    13441 cgtcttgggc gcgataatag cgagctggag tggcgcgagc acggctttaa aaacggcgtc
    13501 ttttttgccc aggctaaagg tcggctaatt atcgacggta tcgaggcgct gaaatccgcg
    13561 ttctggaatt tctcttcatt ctcgctggaa actgtcgctc aggagctatt aggcgaagga
    13621 aaatctatcg ataacccgtg ggttcgaatg gacgaaattg accgccgttt cgccgaagat
    13681 aaacctgcgc tggcaactta taacctgaaa gattgcgagc tggtgacgca gatcttccac
    13741 aaaactgaaa tcatgccatt tttactcgaa cgagcaacgg tgaacggcct gccggtggat
    13801 cggcacggcg gttcggtggc agcgtttggt catctctatt ttccgcgaat gcatcgcgct
    13861 ggttatgtcg cgcctaatct cggcgaagtg ccgccgcacg ccagccctgg cggctacgtg
    13921 atggattcac ggccagggct ttatgattca gtgctggtgc tggactataa aagcctgtac
    13981 ccgtcgatca tccgcacctt tctgattgat cccgtcgggc tggttgaagg catggcgcaa
    14041 cctgatccag agcacagtac cgaaggtttt ctcgatgcct ggttctcacg ggagaaacat
    14101 tgcctgccgg agattgtgac taacatctgg cacgggcgcg atgaagccaa acgtcagggt
    14161 aataagccgc tttcgcaggc gctgaagatc attatgaatg ccttttatgg cgtgctcggc
    14221 accaccgcct gccgcttctt cgatccgcgc ctggcatcgt cgatcaccat gcgtggtcat
    14281 cagatcatgc ggcagaccaa agcattgatt gaagcgcagg gctacgacgt tatctacgga
    14341 gataccgact cgacttttgt ctggctaaaa ggtgcacatt cggaagaaga ggcggcgaaa
    14401 atcggtcgtg cactggtgca gcacgttaac gcctggtggg cggaaacgct gcaaaaacaa
    14461 cggctgacca gcgcattaga actggagtat gaaactcatt tctgccgttt tcttatgcca
    14521 accattcgcg gagctgatac cggcagtaaa aagcgctacg ccgggctgat tcaggagggc
    14581 gacaagcagc ggatggtgtt taaagggctg gaaaccgtgc gcaccgactg gacgccgctg
    14641 gcccagcagt ttcagcagga gctgtacctg cgtatcttcc gcaacgagcc atatcaggag
    14701 tacgtccgcc aaaccatcga caaactgatg gcgggtgaac tggatgcgcg gctggtttac
    14761 cgtaaacgcc ttcgccgtcc gctgagcgaa tatcagcgta atgttccgcc tcatgtacgc
    14821 gccgctcgcc ttgccgatga agaaaatcaa aagcgcggtc gccccttgca atatcagaat
    14881 cgcggcacca ttaagtacgt atggaccacc aacggcccgg agccgctgga ctaccaacgt
    14941 tcaccactgg attacgaaca ctatctgacc cgccagctac aacccgtggc ggagggaata
    15001 ctccctttta ttgaggataa ttttgctaca cttatgaccg ggcaacttgg gctattttga
    15061 gcaaaaaaag agttcgccag ataccatttt gatgcgtgac gaatgctttg ccatccagta
    15121 ccatagcgcc ctttccattc ctggacctga ataacaccac tacctcataa gcacggtagc
    15181 gggtggttat tgcctgcaat taaagatata gagccgaaca catatgcctt ttacacttgg
    15241 tcaacgctgg atcagcgata cagaaagcga attgggactt ggaaccgttg tcgcggtgga
    15301 tgcgcgaact gtcactttac ttttcccatc tactggtgaa aaccgtctgt acgcacgcag
    15361 tgattccccc gtgacccgcg tgatgttcaa ccctggtgat accattacca gccatgacgg
    15421 ctggcagatg caagtcgaag aagtaaaaga agaaaatggc ttgctgacct atatcggtac
    15481 tcgcctggat actgaagagt ccggcgtagc cctgcgtgaa gttttccttg atagcaaact
    15541 ggtgttcagc aaaccgcagg accgtctgtt tgccgggcag attgaccgta tggaccgctt
    15601 tgcgctgcgt tatcgcgcgc gtaagtattc cagcgaacag ttccgtatgc cgtacagcgg
    15661 cctgcgcggt cagcgtacca gcctgatccc gcatcagctc aacatcgctc atgatgttgg
    15721 tcgccgccac gcgccgcgcg tcctgctggc tgacgaagtg ggtttaggga aaaccattga
    15781 agccgggatg atcctgcatc agcaactgct ctctggcgct gctgaacgtg tgctgattat
    15841 cgtcccggaa accttacagc atcagtggct ggtagaaatg ctgcgccgtt tcaacctgcg
    15901 ctttgctctg tttgatgatg agcgttatgc cgaagctcag cacgatgctt acaacccgtt
    15961 tgacaccgaa cagctggtga tttgctcgct ggattttgcc cgtcgtagca aacagcgcct
    16021 ggaacatctc tgtgaagccg aatgggacct gctggtggtc gatgaagcgc atcacctggt
    16081 gtggagcgaa gatgcgccaa gccgtgaata tcaggccatt gaacaactgg cagagcacgt
    16141 gccgggcgtt ctgctgctga ccgcgacccc ggaacagctg gggatggaaa gccacttcgc
    16201 ccgtctgcgt ctgctggacc cgaaccgttt ccacgatttt gcgcagttcg ttgaagagca
    16261 gaaaaattat cgtccggttg cggacgccgt tgccattctg ctggcaggta acaaactgag
    16321 caatgacgaa ctgaacatgc tcggcgagat gatcggcgag caggatatcg agccgctgtt
    16381 gcaggcagca aacagcgaca gcgaagatgc ccagagcgcc cgtcaggagc tggtttcgat
    16441 gctgatggat cgccacggca ccagccgcgt gctgttccgt aacacgcgta acggtgtgaa
    16501 aggattcccg aaacgcgagc tgcacaccat taagctgccg ctaccgacgc agtatcagac
    16561 ggctattaaa gtctccggca ttatgggcgc acgtaaaagt gcggaagatc gtgctcgcga
    16621 tatgctctac ccggagcgta tttatcagga atttgaaggt gataacgcca cctggtggaa
    16681 cttcgatccg cgcgttgagt ggctgatggg ctacctgacc agccatcgct ctcagaaagt
    16741 gctggtgatc tgcgccaaag ctgccactgc gctgcaactg gagcaggtac tgcgcgaacg
    16801 tgaaggtatt cgcgctgcgg tgttccacga aggtatgtcg attatcgaac gtgaccgcgc
    16861 tgccgcctgg tttgccgaag aagacaccgg cgcacaggta ctgctgtgct cagaaatcgg
    16921 ttctgaagga cgtaactttc agttcgccag ccacatggtg atgtttgacc tgccattcaa
    16981 cccggatcta ctggagcagc gtattggtcg tctggatcgt atcggccagg cgcacgatat
    17041 tcagatccat gtgccttatc tggagaaaac cgctcagtcg gtgctggtgc gctggtatca
    17101 cgaaggtctg gatgcatttg agcacacctg cccgaccgga cgcactattt acgatagcgt
    17161 atacaacgat ctgattaact atctggcttc accggatcaa accgaaggct ttgacgatct
    17221 gatcaaaagc tgccgcgagc aacatgaagc gctgaaagca cagctggaac agggtcgtga
    17281 ccgcctgctg gaaatccact ccaacggtgg cgaaaaagcc caggcactgg cagaaagcat
    17341 tgaagagcag gatgacgata ccaacctgat cgccttcgcc atgaacctgt tcgatattat
    17401 cggtatcaat caggacgatc gcggcgacaa catgatcgtg ctgacgccgt ccgatcatat
    17461 gctggtgccg gacttccctg gcctgtcgga agatggcatc accatcacct ttgatcgtga
    17521 agtggcgctg gcgcgtgaag atgcacagtt tattacctgg gagcatccgc tgatccgcaa
    17581 cggtctggat ctgatccttt ctggcgatac cggtagcagc acgatttcac tgttaaaaaa
    17641 caaagcgttg ccggtaggta cgctgttggt ggaactgatt tatgtggttg aagcccaggc
    17701 tccgaagcag ttgcagctca accgcttcct gccaccgacg ccggtacgta tgctgctgga
    17761 taaaaacggc aacaacctgg cggcgcaggt agagtttgaa acctttaacc gccagcttaa
    17821 cgcggttaac cgtcacaccg gcagcaaact ggttaacacc gtgcagcagg atgttcacgc
    17881 tatccttcaa ctgggtgaag cgcagatcga gaaatctgcc cgtgcattga ttgatgcagc
    17941 gcgtaacgaa gccgacgaaa aactgtctgc cgagctgtct cgtctggaag ctctgcgtgc
    18001 agtgaacccg aacattcgtg acgacgaact gaccgccatt gagagcaacc gtcagcaggt
    18061 aatggaaagc ctggatcagg caggttggcg tctggatgcc ctgcgtttga tcgttgtaac
    18121 gcatcagtaa cggagccgaa aatggggatg gaaaactaca atccaccgca ggaaccctgg
    18181 ttggttatcc tgtatcagga tgaccatatt atggtggtca acaagccgag cggtttgttg
    18241 tcagtgccgg gtcgtctgga agagcacaaa gacagcgtga tgacgcgcat tcagcgtgat
    18301 tatccgcagg cagaatcggt gcatcgtctg gatatggcta ccagcggcgt gattgtagtg
    18361 gcgctgacca aagccgcgga gcgggagtta aaacgccagt tccgcgagcg cgagccgaaa
    18421 aagcagtatg tggcccgcgt ctgggggcat ccatcccccg cagaaggtct ggtggatctg
    18481 ccgctgattt gcgactggcc aaaccgcccg aaacagaaag tctgttacga aacgggtaaa
    18541 cctgcgcaga cggaatatga agtggtggag tatgcggcgg ataacacggc aagagcggtg
    18601 ttaaaaccga ttaccgggcg ttcgcatcaa ttgcgtgtgc atatgctggc gctggggcat
    18661 ccgattctcg gcgatcgttt ttatgcatca ccagaagcga gagcgatggc accgcgtttg
    18721 ttgctacatg cagagatgtt gacgattacc catccggcgt atggcaatag tatgacgttt
    18781 aaagcgccag cggattttta atcgtcacgc tggcgcagtt cggtaggcgg ataaggcgtt
    18841 cacgccgcat ccggcaattg tgcgccgatt gcctgatgcg acgcgggccg cgtcttatca
    18901 ggcctacagt ttaaaactta tttaaaccct ttctgctgct ttatcagctc atatgcctgc
    18961 tgaatttcct gcgctttctg cttcgccatc tccatcatct caggcggcaa acctttcgcc
    19021 accagcttat cgggatggtg ttcactcatc agcttacggt aggcacgttt gatggtggtc
    19081 gcatcatccg tcggcttcac gcccagcaca ttacaggcat cttccagcgt cgggccacgc
    19141 tgcgcttgct gccagttacc accgccagat tgctgctgat aaccgccgcc aaactgtgca
    19201 ccgccctgca tcatgcgcaa aaactggtca aactgggcgc gggagatccc taattcttca
    19261 gcaatgacat acagcaccgc ccgttcattc gggtgcagtg aaccatcagc aaacgccgcc
    19321 tgaatctgga tctccagaaa catacgaatt aagtcaaaac gaccaaagca gacactgcga
    19381 aactggcgca tcttttcgcg cagcgggtaa ttatctgatt ttcccacccg gaacgcgttt
    19441 tgcgccgcag tacgggaagc gccatgaagg ttcattcggt ccatcaactg gctggcgata
    19501 tgaatatcag cctccgtgac gcgacctttg gatttggtta aatgccccat cacttcaaaa
    19561 gtggtggcaa aaaacagcgc ctgacgctca cgctggttgg cgaaccacgc cattttacgg
    19621 ctacgggctt tatcaaacat atggccaatt aacaggccta acactacgcc ccaaaagccg
    19681 ccgcccatca gtaaggccac ggccacgcca atgatttttc cccaatactg catatattcc
    19741 ccaaatcgac acacggatat cagggctatc tcccacaata taaaggtgct tttaccgttt
    19801 tccggctgcg gtcaattgtg ggacatcgcc tataatttgc attatcatac ctgtcattca
    19861 atgccgtgcc taacaccact gacgctattc ggacaggatt aacactagcg tagagatgac
    19921 aagtacgtta gtctctgacc gtttgtcacg cgcaacgtta ccgatgatgg aacaataaaa
    19981 tcaacgtatg aaaaaacgta tccccactct cctggccacc atgattgcca ccgcccttta
    20041 tagtcaacag ggactggcag ccgacctcgc ctcacagtgc atgttgggcg tgccaagcta
    20101 tgaccgtcct ctggtacagg gcgataccaa tgacttaccc gtgactatca atgctgacca
    20161 cgcgaaaggg gactacccgg atgacgccgt gtttactggc agcgtggata tcatgcaggg
    20221 taacagccgt ctgcaggccg acgaagtgca gctccatcaa aaagaggcac caggacaacc
    20281 ggagccggta cgtaccgttg atgcgctcgg taatgtccat tacgacgata accaggtgat
    20341 cctcaaaggg ccgaaaggct gggcgaatct gaacaccaaa gataccaacg tctgggaagg
    20401 tgattaccag atggtgggtc gccagggtcg cggtaaagcg gacctgatga aacaacgtgg
    20461 cgaaaaccgc tataccattc tggataacgg tagctttacc tcctgtctgc cgggttctga
    20521 cacctggagc gtggtaggta gcgaaattat tcatgaccgc gaagaacaag ttgcggagat
    20581 ctggaacgcc cgctttaagg tgggtccggt accgatcttt tatagcccct atttgcagtt
    20641 gccggtgggt gacaaacgtc gctctggttt cttgatcccg aacgccaagt acaccaccac
    20701 caactacttt gagttctacc tgccatatta ctggaacatc gcgccaaata tggatgccac
    20761 catcacgccg cattatatgc atcgtcgtgg caacatcatg tgggagaacg aattccgcta
    20821 cctctcccag gcgggcgctg gcttgatgga actggactat ctgccttcag ataaagtcta
    20881 tgaagatgaa cacccgaacg atgacagttc acgtcgttgg ttattctact ggaaccactc
    20941 cggggtcatg gatcaggtgt ggcgtttcaa cgtcgactac accaaggtca gcgatcctag
    21001 ctacttcaat gatttcgata acaagtacgg ttccagtact gacggctacg caacgcaaaa
    21061 attcagcgtt ggctatgcgg tgcaaaactt caatgccacc gtttcaacca agcagttcca
    21121 ggttttcagc gaacagaaca ccagtagcta ctcggcagag ccgcagttag acgttaatta
    21181 ctaccagaat gatgttggtc cgtttgatac gcgtatttac ggccaggcag tgcactttgt
    21241 taacaccaga gacgacatgc ctgaagcaac ccgtgttcac ctggaaccga ccatcaattt
    21301 gccgctctct aataactggg gcagcatcaa taccgaagcg aagtttctgg caacccatta
    21361 tcagcaaacc aatcttgact ggtataactc cagaaacacg accaagctgg acgaatccgt
    21421 taaccgcgta atgccgcaat tcaaagttga cggcaaaatg gtctttgaac gcgatatgga
    21481 aatgctggct ccgggttata cccaaacgct ggaaccgcgc gcgcagtatt tgtacgtgcc
    21541 gtatcgcgat cagagcgaca tctataacta cgactcgtct ctgctgcaat ctgactactc
    21601 tggcctgttc cgggaccgga cttacggcgg tcttgaccgt attgcctccg ctaaccaggt
    21661 gacgaccggt gtcacatctc gcatatatga tgatgctgcc gttgaacgtt ttaatatttc
    21721 cgttggtcaa atctactatt tcacggagtc tcgcactggc gatgacaaca taacatggga
    21781 gaatgacgac aaaacgggct cactggtgtg ggcaggcgac acctactggc gtatctccga
    21841 gcgttgggga ttgcgtggcg ggattcagta cgatacacgt ctggataacg tagcgaccag
    21901 taactccagc attgaatacc gtcgggatga agaccgtctg gtacagctga attaccatta
    21961 cgccagcccg gaatatattc aggctacgct gcctaagtac tattccactg ctgagcaata
    22021 taagaatggt atttcgcagg taggtgctgt cgccagcagg ccaattgccg atcgttggtc
    22081 cattgttggg gcctactact acgacaccaa tgcgaacaag caagccgact ctatgttagg
    22141 tgtgcaatac agctcctgct gctatgcaat tcgcgtcggt tacgagcgga agctgaacgg
    22201 ttgggataac gataaacaac atgcggtata tgacaacgca atcggcttta acatcgaact
    22261 tcgcggcctg agctccaact acggtctggg tacgcaagag atgctgcgtt cgaacattct
    22321 gccgtatcaa aacactttgt gatttgttga tttaccacgt aatccgcagt gcggttaatt
    22381 gaaatggaaa aagtatgaag aactggaaaa cgctgcttct cggtatcgcc atgatcgcga
    22441 ataccagttt cgctgccccc caggtagtcg ataaagtcgc agccgtcgtc aataacggcg
    22501 tcgtgctgga aagcgacgtt gatggattaa tgcagtcggt aaaactgaac gctgcgcagg
    22561 caaggcagca gcttcctgat gacgcgacgc tgcgccacca aatcatggaa cgtttgatca
    22621 tggatcaaat catcctgcag atggggcaga aaatgggagt gaaaatctcc gatgagcagc
    22681 tggatcaggc gattgctaac atcgcgaaac agaacaacat gacgctggat cagatgcgca
    22741 gccgtctggc ttacgatggt ctgaactaca acacttatcg taaccagatc cgcaaagaga
    22801 tgattatctc tgaagtgcgt aacaacgagg tgcgtcgtcg catcaccatc ctgccgcagg
    22861 aagtcgaatc cctggcgcag caggtgggta accaaaacga cgccagcact gagctgaacc
    22921 tgagccacat cctgatcccg ctgccggaaa acccgacctc tgatcaggtg aacgaagcgg
    22981 aaagccaggc gcgcgccatt gtcgatcagg cgcgtaacgg cgctgatttc ggtaagctgg
    23041 cgattgctca ttctgccgac cagcaggcgc tgaacggcgg ccagatgggg tggggccgta
    23101 ttcaggagtt gccggggatc ttcgcccagg cattaagcac cgcgaagaaa ggcgacattg
    23161 ttggcccgat tcgttccggc gttggcttcc atattctgaa agttaacgac ctgcgcggcg
    23221 aaagcaaaaa tatctcggtg accgaagttc atgctcgcca tattctgctg aaaccgtcgc
    23281 cgatcatgac tgacgaacag gcccgcgtga aactggagca aattgctgct gatatcaaga
    23341 gtggtaaaac gacttttgct gccgcagcga aagagttctc gcaggatcca ggttctgcta
    23401 accagggcgg tgatctcggc tgggctacac cagatatttt cgatccagcc ttccgtgacg
    23461 ccctgacccg cctgaacaaa ggtcaaatga gtgcaccggt tcactcttca ttcggctggc
    23521 atttaatcga actgctggat acccgtaatg tcgataaaac cgacgctgcg cagaaagatc
    23581 gtgcataccg catgctgatg aaccgtaagt tctcggaaga agcagcaagc tggatgcagg
    23641 aacaacgtgc caacgcctac gttaaaatcc tgagcaacta atggttaaaa cccaacgtgt
    23701 tgtgatcact cccggcgagc ccgccgggat tggcccggac ttagttgtcc agcttgcaca
    23761 gcgtgagtgg ccggtcgaac tggttgtttg tgccgatgcc actctcctta ccgaccgggc
    23821 agcgatgctc ggtttgccgc tcaccctccg cccttattcc cccaactccc ctgcacaacc
    23881 gcaaactacg ggcacattaa cgctacttcc tgtcgcgcta cgtgaatctg tcactgcggg
    23941 gcagttagcg attgaaaatg ggcattacgt ggtggagacg ctggcgcgag cgtgtgatgg
    24001 ctgtctgaac ggtgaatttg ctgcgctgat cacaggcccc gtgcataaag gcgtcattaa
    24061 cgacgcaagc attccgttta ccggtcatac cgagtttttc gaagagcgtt cgcaggcgaa
    24121 aaaagtggtg atgatgctgg cgaccgaaga acttcgcgtg gcgctggcaa cgacgcattt
    24181 accgctgcgc gatatcgcag atgctatcac ccctgcactt ttgcacgaag tgattgctat
    24241 tttgcatcac gatttgcgta caaaatttgg tattgccgaa ccgcgcattc tggtctgcgg
    24301 gctgaatccg cacgcgggcg aaggcggtca tatgggtacg gaagagatag acaccattat
    24361 tccggtgctc gacgagctgc gggcgcaggg aatgaaactc aacgggccgc tgcctgccga
    24421 taccctgttt cagccgaaat atctcgataa cgccgacgcc gtgctggcga tgtaccacga
    24481 tcagggtctt cccgtgctaa aataccaggg cttcgggcgc ggtgtgaaca ttacgctggg
    24541 cctgcccttt attcgcacat cagtggacca cggcaccgcg cttgaactgg cgggacgtgg
    24601 cgaagccgat gtcggcagtt ttattacggc gcttaatctc gccatcaaaa tgattgttaa
    24661 cacccaatga ataatcgagt ccaccagggc cacttagccc gtaaacgctt cgggcaaaac
    24721 tttctcaacg atcagttcgt gatcgacagc attgtttctg ccattaaccc gcaaaagggc
    24781 caggcaatgg tcgaaatcgg ccccggtctt gcggcattga ccgagccggt cggcgaacgt
    24841 ctggaccagc tgacggtcat cgaacttgac cgcgatctgg cggcacgtct gcaaacgcat
    24901 ccattcttag gcccgaaact gacgatttat cagcaggatg cgatgacctt taactttggc
    24961 gaactggccg agaaaatggg tcagccgctg cgtgttttcg gcaacctgcc ttataacatc
    25021 tccacgccgt tgatgttcca tctgtttagc tatactgatg ccattgccga catgcacttt
    25081 atgttgcaaa aagaggtggt gaatcgtctg gttgcaggac cgaacagcaa agcgtatggt
    25141 cgattaagcg tcatggcgca atactattgc aacgtgatcc cggtactgga agttccgccg
    25201 tcagccttta caccaccacc caaagtggat tccgccgtcg tgcgcctggt ccctcatgca
    25261 acgatgcctc acccggttaa agatgttcgc gtgttgagcc gcatcaccac cgaagccttt
    25321 aaccagcgtc gtaaaaccat tcgtaacagc ctcggcaacc tgtttagcgt cgaggtgtta
    25381 accggaatgg ggatcgaccc ggcgatgcga gcggaaaata tctctgtcgc gcaatattgc
    25441 cagatggcga actatctggc ggagaacgcg cctttgcagg agagttaacg atgatcaatt
    25501 cgccccgagt gtgtattcag gttcaaagcg tctacattga ggcacaatct tcacctgata
    25561 atgaacgtta cgtttttgct tataccgtaa ccatacgcaa tctggggcga gcgccagtgc
    25621 agttgttggg gcgttactgg ctgatcacca atggcaatgg ccgtgaaacc gaagtccagg
    25681 gcgaaggagt ggttggcgtc cagccactta tcgcgcctgg cgaagagtac cagtacacca
    25741 gcggtgcaat cattgaaacc ccgctgggca ccatgcaggg tcactacgaa atgatcgatg
    25801 aaaatggcgt ccctttcagc atcgacattc ccgtattccg actcgccgtt cccacactca
    25861 ttcattaaaa gaatatggcg acatacctta ttggcgacgt tcatggttgt tacgatgaac
    25921 tgatcgcatt gctgcataaa gtagaattta cccccgggaa agataccctc tggctgacgg
    25981 gcgatctggt cgcgcgcggc ccgggttcgc tggatgttct gcgctatgtg aaatccttag
    26041 gcgacagcgt acgtctggtg ctgggcaatc acgatctgca tctgctggcg gtatttgccg
    26101 ggatcagccg caataaaccg aaagatcgcc tgacaccgct gctggaagcg ccggatgccg
    26161 acgagctgct taactggctg cgtcgccagc ctctgctgca aatcgacgaa gagaaaaagc
    26221 tggtgatggc ccacgcaggg atcacgccgc agtgggatct gcagaccgcc aaagagtgcg
    26281 cgcgcgatgt agaagcggtg ctgtcgagtg actcctatcc cttctttctt gacgctatgt
    26341 acggcgatat gccaaataac tggtcaccgg aattgcgggg gctgggaaga ctgcgtttta
    26401 tcaccaacgc ctttacccgt atgcgttttt gcttcccgaa cggtcaactg gatatgtaca
    26461 gcaaagaatc gccggaagaa gcccctgccc cactgaaacc gtggtttgcg attcctggcc
    26521 ctgtcgctga agaatacagc atcgcctttg gtcactgggc atcgctggaa ggcaaaggta
    26581 cgccggaagg tatttacgcg ctggataccg gctgctgctg gggtgggtca ttaacctgcc
    26641 tgcgctggga agataaacag tattttgtcc agccgtcgaa ccggcataag gatttgggcg
    26701 aagcggcggc gtcttaaaca cagcctgata caggaaggcc ggataagacg cgaccggcgt
    26761 cgcatccggc actagccgta aattctatac aaacttaccg ccgctccaga atctcaaagc
    26821 aatagctgtg agagttctgc gcatcggcgt cgtggaattc gctgaatacc gattcccagt
    26881 catccggctc gtaatccggg aaatgggtgt cgccttccac ttctgcgtcg atatgcgtca
    26941 gatacagttt ttgcgctttt ggcaggaact gctcataaac gcgaccgccg ccaatcacca
    27001 tgatttctgg tacgtcacca cacgccgcaa tggcttcatc caccgatttc acccacgtta
    27061 cgcgatcgtc cgtacccggt tgactgctga ggataatatt tttgcgtcct ggcaacggac
    27121 gaccgattga ttcccaagta tggcggccca taatcacggg tttatttaag gtgttgcgtt
    27181 taaaccaggc gagatcggca ggcaggttcc acggcatggc gttttccatg ccgataacgc
    27241 gatctaccgc taacgccgca atcagactga tcattgagat ttcccgataa aaaaattgtc
    27301 gccactatac gtaaagcgta aaccgtcgtc gactggtgcg aggatgatgt tgaggaaaat
    27361 tttatattct gctggcgagt ccacgctctc ccccaggact cgccgcatta caatgaaaca
    27421 aaaacaaaca gttagctgta aagtgtgatt tacgtcactc tttattaaga tgagggtttc
    27481 gtttccggtt catccgccat gttgccggta tgtttacctt cttctgttcc ctgccagcca
    27541 tgtcgttgaa ttaatgacag atgttcgcgg tcctcggtga tgatctcact taacatcgcg
    27601 ctggtgcgtt tatagaccgc cgcgcgggct ttggtgtcat tctcaaccat tgccatctct
    27661 tccaccatct gaatattaaa gcggcggaac acatcggcac gttctcgcgc ttcatacggc
    27721 cccagaccta aactttccag cgccagacgc ccggttttca gcgcaccttc aaaggtttca
    27781 cgctccggct tttcaacgcc tgcctgacgc aaacgaatgt agtgatcgac atcgcgggcg
    27841 cgggcaataa tctgcaaatg cgggaaatgt tctttcacca tctccgtcag ttgcaggttg
    27901 gtttgcggat cgtcaatagc gttaatcagc acttccgctt tcgccgctcc ggcagattcc
    27961 agtaaatcca tccgcgtggc atcgccataa aacactttca taccaaattt acgcagcgtt
    28021 tcgatatggt ccggatcgtg atcgagcacc accattttca ccccgctgga gagcagtaaa
    28081 cgtccggtaa tctgtccaaa acgaccgaat ccggcgataa tcacgcgcgg ctgttcttca
    28141 tcgatctcat cggcttcacg cgcctcctcc gtagaagatt gctcaaggcg attgaggatc
    28201 accagcagaa tcggcgttgc tgccatcgac agcgccaccg ccagggtcag cgatttcgcc
    28261 cactccggct ccagcacatt cgccatctgc gccgcaccaa ataccacaaa ggcaaactca
    28321 ctgccctgcc ctaacaacac cgcaaaccag cgacgctgtt tatttggcac ctgcaacggt
    28381 cgggcaatca gccacagcat ggcgattttg atgatgagga aaccgagcag caaaatgaca
    28441 atgcgcaatg ggttttcaat cagcgtgcca aagtctatcg acatgccaac accgatgaaa
    28501 aacagcccca acagcaaacc tttaaacggt tcgatatcgc tttccaacgc atgacggtat
    28561 tccgagcttg ccagcagtac gcccgccaga aacgcgccca tcgccatcga caagccgacc
    28621 tcttccagca gcaaaccaaa gccaaacaca aggaataacg ccacggcact aaacacttcc
    28681 cgcaagccag agcgggcaac aaaacgcagc gccggacgcg taacatagcg ccccagcaat
    28741 accaccagta ccagcgcacc cgccactttt aacgccgaga gagcaaatgc gcccatcgtc
    28801 gtcgaggcac tgctcgccgc cagtagcggg atcatcgcca ccagcgggat tgccgcgata
    28861 tcctggaaca gcagcactgc aaaggcactg cggcccattt gcgtcaccat cagattgcgt
    28921 tcattcatcg cctgcatggc aatcgccgtt gatgagagcg ccagcgtcat accgatcaat
    28981 tccgcgacct gccagcgcag cccaagtaac atacagaaca gccccagcag gccaccgcaa
    29041 atcaccatct gcaatgcgcc accgccgaac actgccgcac gcagcttcca cagcctttgt
    29101 ggatcgagtt cgaggccgat aataaacagc atcagcacca ccccaatctc ggcaaagtgc
    29161 agaatagatt cggcatcggt caccagtcgc agcccccacg ggccaataat gcagccggcg
    29221 atcaggtagc caagtaccga tcccagacca agacgtaccg caatgggtac aatcagcgcc
    29281 gccgatccga ggtagatcag cgcctgaacc agcgtatgac tatccatgat gggcctcctg
    29341 ccattccagc agacgttgct tatagtggcg cgcctgccct tcgagggttt cgtcgtcaca
    29401 aataaaggtg cagtgcatgg caaacggtgg cagccagttc agcccgcagt agattgccgt
    29461 cgcctgtagc ggctgcgaca gcacatcaaa gcccggatgc gcaccaattt caaaatggct
    29521 ttccccgccg ccggtcgtca ccgcccacag caaatgtttg ccatgcagcg ccgtgccgcc
    29581 gtgaccgtaa gcccagccgt gcgacaaaac tttatcgatc caaagtttaa gaagcggagg
    29641 aatgctgtac cactgcatcg ggtgctgcca gacgatcaga tcggcgcgag agagcgcctc
    29701 ctgctcggcg gcaatatcga tattgaagtc aggatagagt tgataaagag agcgaatttc
    29761 gacgccttcc agcgtccttg cctgttcaag catccgttta ttcgcatggg aatgatgcgg
    29821 atacggatgc gcataaatga tgaggatcat agttattagc ccgcgtgttt acgcctttgt
    29881 tttcccacaa agtgtagtca tatattcacg cggcttatag tcaataatat tgattcagaa
    29941 aagagagaaa tctgaacaaa taatcccatt ctctgcccgc ggatcgtcat attgctggtc
    30001 tgcgccagtg acattcctcg agtctccggg gcaaacgcta cggaaatcaa caagccaaac
    30061 agcgagatac ccgcccccat tagcatcgtg ttactgatac cgtaattatt gataaatatc
    30121 ggcagtgccc aggtcgaaac aatggtgcca atacgactta aggacataat cacgcccacg
    30181 gcagaggcgc ggatatccgt cgggaagagt tcattaggat agagccactg caaattaccc
    30241 ggcccgccag agaaaaaggc atacaccgca aaggccatca ctaccagcca gatccccata
    30301 tccgggatta gccccaaaac cgccagcgcc agcgtcatca tggcaaagct gccaatcaac
    30361 aatggacgcc gtccggcagt gtttagccac agcatcggcg gaatacagcc gagcataaag
    30421 aacaggctaa tcaccacatt ccctagtgcc gcgtttttgc caacccccaa tcccaacgaa
    30481 ccaacgattt gcgggccaaa ggtgtaaatg gcgaacattg ggatcacctg gcaggtccag
    30541 atggcggcaa caaacagaac aaaaggaaaa tggcggcgat taaacagatc gcgaaaacgg
    30601 gtttgctgcg gctgctcttc atcgaaagcc accggttcgc caaacagttt tatcatcatc
    30661 tcttcgcact cttttactcg ccctttgcgt aataaccagc ggggagattc tggcagttcg
    30721 aatcgaccaa tcaaaatcaa caaacaaggg atcgccgcgc tacccagcat ccagcgccaa
    30781 ccaccttcca catcataaag ccagtagccg accagatcgg cgcaggtcgc gccgacatac
    30841 cacatcgcgg caataaagct gatggaaaac gcccgctgac gggtactgga gaactcggtg
    30901 atcatcgaag tggcgatggg ataatctgca ccgatgacaa tgccgatgag tacccgcatc
    30961 accaacagtt cgacggggga tgaaacacac atcgtcgcca ccgatatcac gccgatggcg
    31021 atgatatcaa tgaggaacat tttgcgccgt ccgactttat cggagatata gccaaacagc
    31081 gatgtgccaa cgaacagccc ggcgagcgtt cccgcgccca gcaagccaat ccagtcagcg
    31141 tccagtttca gcgccggagt cagttgctcc agcgccacgc caatcattac cagtacataa
    31201 ccatccagaa acggaccacc gcttccccac agcaaaatgc ggcggtgaat agaggagaat
    31261 ttgagatcgt caaagtttct ggacggttgc atgggttatt cctgtttttt tgcaatgaga
    31321 gtcagtacgg cgttacgtat caaaccaaca tcagccgtaa cggaactcca caccaaaggt
    31381 gccgcgcggg tattcccact gttccagcgc cgaccccagc cccagaatgc gacaggtgcc
    31441 gcactccaga catccggcgt aatcgaagcg cacactgccg tcatcctgct tcttgtacag
    31501 acctgcgggg cacgctttca ccagcagctc cagcacctgt ttatcagcat cggccttcac
    31561 aacaatgtgc ggatgctctt catcgacatt gaatttattg acgcccagtt tgacgtccac
    31621 attgacggga gaagtcataa aacggtcact cctttcatgc catccttgat cagattgatg
    31681 aagcccactt tcttgccgtg gcggaggatt ttcttgcgca tcagttccgg cgcactgccg
    31741 tcaatggtga acaggtcacg cgccacgccc accgccagtt ccgggtagcc gctaaacatg
    31801 cgtgggttat caaggaacgc cggtagtttc tggtacatgc gcatatcgcg cagcgggctg
    31861 ctctccagat gctgacgata ttccgcgagt ttttgcttac tgaaatcgtc gcttttcatc
    31921 gctgaaagca cggtttttgc tgcagcttcc ccggcggcaa tcgccagatc cataccgcga
    31981 atggtaaaac cgaggttcat acacattccg gcggcatcac cggcaatcaa tacgccgtca
    32041 ccgaccagct ccggcagcat gttgatgcct gcttccggca ctacgtgagc ggaatattcc
    32101 accagcttgc cgcccgcgat cagcggtgca acggccggat gctgtttgaa atcttccagc
    32161 atttgcggca ccgatttttt cgcgtcatgc agatgatgca gaccacaaac cagccccagc
    32221 gacagggtat tttcattggt ataaaggaag ccgccgccca tcaggccatc ggtgggtgat
    32281 cccgcaaaca gacaagccgc gccctgatta ccctgcaact gaaaacggtc ttcgattacc
    32341 gacttcggta actcgatcag ttccttcacg ccaaccgcca catccgtcgg tttaacgcgt
    32401 tttgccatcc ccaacttttc ggcaaggatg gagttcaccc catcagcaag gagcaccgtt
    32461 ttcgcttcaa tcacatcgcc atcggcttct acaccgacga ctttgccatc gcgctgtacg
    32521 aggttatcga cgcggatccc ggtaattaac tgcgcgcccg cttcttcggc ctgctccatc
    32581 aaccaggcat caaatttact gcgcaaaacg gagtaagaac gctgggatag cgaggtttcg
    32641 tcaccgttgc agtagtccat agtcatcgct gacttttccg tcataaacgc gagtttttca
    32701 tgggtgatca ggcgttctac gggggcggaa tcggcgaaac ctggaataat gtgttccaga
    32761 ctgtgggcat agagacgccc gccagtaacg ttcttggcac ccgcggaatt gccacgctcg
    32821 ataactaaca cttgcgcccc ttcgcgggcg agcaccagtg cggcaaccga accggcaagc
    32881 cctgcaccga cgatgatggc gtcaaagata tcttcggaca taagagctcc ctgtcagcgg
    32941 cagagccgta atgcagccct gccagagtgg atcaacgcgc taaagctgcg gtcagtgccg
    33001 gaaggatctt cacggcatcg ccaacaatgc cgtaatccgc gtactggaag atcggcgcat
    33061 ttttatcttt attgatggcg aaaatggttt gcgacgcgtt agcgccaacc atgtgctgga
    33121 tctgcccgga gatccccacc gccaggtaca gttcaggttt cagcatcagg ttggagatac
    33181 cgacatagcg ttcgtgctcc atccattttt cgttttctgc caccggacga gaacaggcca
    33241 actccgcacc tatcgccttg caaagctgtt ctgccagtgc aatgttctct ttgctgccaa
    33301 tgccgcgacc gacgctgacc accagacggg ctttgtcgag atcgacgctg ttgctctggc
    33361 gcgcctgggt tgccgtgcgg gtaatcgcca cagccggagc ctgccactcc acggtgtgcg
    33421 tttcgccagt gcgtgacgcg tctggctgag ccgcatcgaa cgtgccgctg ctgatggtca
    33481 gtaccgcata cggcgtggca atgcgttctt cgccaatcgc cagaccgccg taaaccatgt
    33541 gtttcactgt tgctttaccg tcctgtacgc tgacggtgct ggcatcgtta gacaccgccg
    33601 ctttaaggcg ataacccagt tttgccgcca gtaatttgcc gcgacgggtg tttggcaaca
    33661 gcaccagacc gtcagcgccg tgctggcgaa tagtgtcagc catgacaccg gcgtaatctt
    33721 cgatcatccg atcgtccggt ttgccgttta atttccagac atgattagcg ccgagctgga
    33781 ttgcctgtgc gccgtcggca tcattgagga caaaggcatt gatttgatta gctaaagcct
    33841 gcgcaccgtt catcagttcc ggcagacgag agggggtatc gctgaatacc cagacttgag
    33901 aaaacgtgtt catagcatcc cctgtaatta aatgactttg cgaagatttt cagcaaatgc
    33961 ggcgatctgt tcttcgccgt cgccttcaat cacgatgcgc tgacgctcgc gctgtttcgg
    34021 cgcggcaacc tgttgttcag accaggcgtt cacggcgtta aaaccaatat ccgccgccga
    34081 ccatacctgg actggctttt tcgccgcgcc gagaatggct ttcatcgaag gaatttgtgg
    34141 ggagttgata tcagtggaaa cagcaacaac cgcaggcagc ggaatgctta aggtttcggt
    34201 ttcatcttcc agttcgcgct caacggtgag ggtatctgcc gtcagggaga taattttgct
    34261 gacgccgtta actgccggaa tattgaggat ttcgcccacc agcagaccaa cctgctgggc
    34321 ataaaggtcg gaagaaccat cgccacagag gatcagatca aagcctgctt tctgggcggc
    34381 tgcagccagt gcgctcgccg tttgttgcgg cagtgcctgc tcgaactggt catcaatcac
    34441 cacaatcagt tcatccgggc cgcgcgatag cacatcttta cgccctttgg cgttggtcag
    34501 ggctttaccg cccacactta aggctgtcac ctgcgcctct gctgcctgtt gctttagctg
    34561 gcaagccgct tcaatagcgt tgagatcgta ttggcttatt ttggcatcgg ctttgctgaa
    34621 gtctaatgaa ccatcagcat tattgaccgc aatatcctgt tcatcaggca cgcacttata
    34681 gcaagtaata atcttcattg catctccaga aatcatgaag gaaacgtcgt tcatatcggt
    34741 agtgttaatt acagatatca cgccctttaa tttggaaata aaactttaat caccaatatt
    34801 gaaaatgtca cacgcagata aatccaactt tcaatattgt taagttcctc accaatattg
    34861 aaaacatggc gtagcaaaaa gaaattttca atattgtttt atagatcacc aatattgaaa
    34921 gtcagatgct attttttacc cacaaaagaa cataacaaac tgattaataa ccaaaattaa
    34981 cgaaaaacgt cctctattca ctcgaaacaa aaatgtgaca ccaatcacag aatacagctt
    35041 attgaatacc cattatgagt tagccattaa cgcgtccacg aggttaataa taattatatt
    35101 aaatgttaac aaaaataaaa caaacgggaa cacaaaataa atattcgttt tcacagtgga
    35161 attaactcat gaagaatgaa aagagaaaaa cgggaataga accgaaggtt ttctttccgc
    35221 cattaataat cgtcggcata ctttgttggc ttacagtcag agatctggat gcggcgaatg
    35281 tcgttattaa tgctgtattc agttacgtca ccaatgtatg gggatgggca tttgaatggt
    35341 atatggtggt gatgcttttc ggttggttct ggctggtgtt tggcccgtat gccaaaaagc
    35401 gtttaggtaa cgaaccgcca gaatttagca ccgccagttg gatctttatg atgttcgcct
    35461 cctgtacgtc tgctgccgta ctgttctggg gatcgattga gatctactac tacatctcca
    35521 ccccgccgtt tggcttagaa ccgaactcga caggagcgaa agagttgggg ctggcttaca
    35581 gcttgttcca ctggggacct ctgccgtggg ccacttacag cttcctttca gtcgccttcg
    35641 cttacttctt ctttgtccgc aaaatggaag tgattcgccc cagctcgaca ctggtgccgc
    35701 tggtaggtga aaaacacgcc aaagggttgt tcggtaccat cgtcgacaac ttctatctcg
    35761 tcgccttgat cttcgcgatg ggtaccagtc tgggccttgc cacgccgctg gtgaccgagt
    35821 gtatgcaatg gttgtttgac attccgcata ccctgcaact ggacgctatc atcattacct
    35881 gctggattat cctcaacgcc atttgcgtcg cttgcggtct gcaaaaaggg gtacgtatcg
    35941 ccagtgacgt gcgtagttac ctgagcttcc tgatgctggg ttgggtgttc atcgtcagcg
    36001 gtgccagctt catcatgaac tacttcaccg attcggtggg gatgttgctg atgtatctgc
    36061 cgcgcatgtt gttctatacc gatcccatcg ctaaaggcgg cttcccgcag ggctggaccg
    36121 tgttctactg ggcatggtgg gtgatttacg ccatccagat gagtatcttc ctcgcccgca
    36181 tctcccgtgg tcgtactgtg cgtgaactgt gcttcggcat ggtgatgggg ctgacagcat
    36241 ccacctggat cctgtggacc gtgctcggta gtaacactct gctgttgatg gataaaaaca
    36301 tcatcaacat tccaaatctg atcgaacagt acggtgtggc gcgcgccatc atcgaaacct
    36361 gggccgctct gccactcagc accgccacca tgtggggctt cttcatcctc tgctttattg
    36421 ccaccgttac gctggttaac gcctgctctt ataccctggc gatgtctact tgccgcgaag
    36481 tacgcgatgg tgaagaacca ccgctgctgg tgcgtatcgg ttggtcaatt ctggttggca
    36541 ttatcggtat tgttctgctg gcgctcggcg gcctgaaacc gattcaaacc gccattatcg
    36601 ccggaggatg cccgctgttc ttcgtcaaca ttatggtgac gctctccttt attaaagacg
    36661 cgaaacagaa ctggaaagat taattaaccc ccaaaatatc aagaggttga aagatggatt
    36721 ttaatttaaa tgatgagcag gaactgtttg tcgccggtat ccgcgaactg atggccagcg
    36781 aaaactggga ggcctatttt gccgagtgcg accgtgacag cgtctacccg gaacgttttg
    36841 tcaaagcact ggcggatatg ggtatcgaca gtctgctgat ccctgaagag cacggtggtc
    36901 tggacgcggg gtttgttact ctcgccgccg tgtggatgga gctgggacgt ctgggggcac
    36961 caacctatgt actgtaccag ctgccgggcg ggttcaatac cttcctgcgc gaaggcacac
    37021 aagagcagat cgacaagatt atggctttcc gcggcaccgg taagcagatg tggaactcag
    37081 cgattaccga accgggcgcg ggctccgacg tgggtagcct gaaaacgact tatacccgta
    37141 gaaatggtaa gatttatctt aatggtagta agtgttttat taccagtagc gcctacaccc
    37201 cgtacatcgt ggtgatggcg cgcgacgggg cttctccgga caaacctgtc tacaccgaat
    37261 ggtttgttga tatgagcaaa ccgggcatca aagtgaccaa acttgagaag ctcggtctgc
    37321 gtatggatag ctgctgtgaa atcacctttg acgatgtgga actggacgag aaagacatgt
    37381 tcggtcggga aggtaacggc tttaaccgcg tcaaagaaga gttcgaccat gaacgtttcc
    37441 tggtagccct gaccaactac ggtacggcga tgtgcgcctt tgaagatgcg gcgcgctacg
    37501 ccaaccagcg cgtgcagttt ggcgaggcta ttggtcgttt ccagttgatt caggaaaaat
    37561 tcgcccacat ggcgatcaaa ttaaactcca tgaaaaacat gctgtatgaa gcagcgtgga
    37621 aagcagacaa cggcaccatc acctctggcg atgcagcgat gtgcaaatac ttctgcgcca
    37681 atgcggcatt tgaagtggtg gatagcgcaa tgcaggtgct gggcggtgtc gggattgcgg
    37741 gcaaccaccg catcagccgc ttctggcgtg acctgcgtgt agaccgcgtt tccggaggat
    37801 ctgacgaaat gcagatcctg acgctgggtc gtgcggtgct gaagcaatac cgctaagtcc
    37861 gtgcgctgcc tgatgcgacg ctgacgcgtc ttatcaggcc tacgggagca cgaatgtaag
    37921 ccggataagg cgtttacgcc gcatccggca gtcatgcgct acataaattt ttcaggagct
    37981 aataatggat catctaccca tgccgaaatt cggaccgctg gccggattgc gcgttgtctt
    38041 ctccggtatc gaaatcgccg gaccgtttgc cggacaaatg ttcgcagaat ggggcgcgga
    38101 agttatttgg atcgagaacg tcgcctgggc cgacaccatt cgcgttcaac cgaactaccc
    38161 gcagctctcc cgccgcaatt tgcacgcgct gtcgttaaat attttcaaag atgaaggccg
    38221 cgaagcgttt ctgaaattaa tggaaaccac cgatatcttc atcgaagcca gtaaaggtcc
    38281 ggcctttgcc cgtcgtggca ttaccgatga agtactgtgg cagcacaacc cgaaactggt
    38341 tatcgctcac ctgtccggtt ttggtcagta cggcaccgag gagtacacca atcttccggc
    38401 ctataacacc atcgcccagg ccttcagtgg ttacctgatt cagaacggtg atgttgacca
    38461 gccaatgcct gccttcccgt ataccgccga ttacttttct ggcctgaccg ccaccacggc
    38521 ggcgctggca gcactgcata aagtgcgtga aaccggtaaa ggcgaaagta tcgacatcgc
    38581 catgtatgaa gtgatgctgc gtatgggcca gtacttcatg atggattact tcaacggcgg
    38641 cgaaatgtgc ccgcgcatga gcaaaggtaa agatccctac tacgccggtt gcggtctgta
    38701 taaatgtgcc gacggctaca tcgtgatgga actggtgggc attacccaaa ttgaagagtg
    38761 ctttaaagat attggcctcg cacatctact cggtacgccg gaaatcccgg aaggcactca
    38821 gcttatccac cgtatcgaat gcccttacgg cccactggta gaagaaaaac tcgatgcctg
    38881 gctggcggca cataccatcg cagaagttaa agaacgcttt gccgaactga atatcgcctg
    38941 tgccaaagta ctgaccgtac cggaactgga aagcaatcca cagtatgttg cccgtgaatc
    39001 aatcactcag tggcaaacga tggatggtcg cacctgcaaa gggccgaaca tcatgccgaa
    39061 attcaaaaat aaccccggac aaatctggcg cggaatgccc tcacatggca tggacacggc
    39121 tgccattttg aaaaatatcg gctacagcga aaacgacatt caggagttgg tcagcaaagg
    39181 tctggccaaa gttgaggact aaacgcgtgg cgtcagtcgg cttgcctgac tagcgcccgc
    39241 ccaataacaa acgaacaaaa atggatagag gtgcaatgga tatcattggc ggacaacatc
    39301 tacgtcaaat gtgggacgat ctggcggacg tttacggtca taaaacggcg ctgatttgtg
    39361 aatccagcgg cggagtcgtt aaccggtata gttatcttga gttaaatcag gagattaacc
    39421 gcacggcaaa cctgttttat acgctgggga ttcgcaaagg caacaaggtt gcactacatc
    39481 tcgacaactg cccggaattt atcttttgct ggttcgggct ggcaaaaatt ggcgcgatta
    39541 tggtgccgat taacgcccgc ctgttacgcg aggaaagcga gtggatcttg caaaatagcc
    39601 aggcgtgcct gctggtgacc agtgcgcaat tttatcctat gtatcaacag attcagcagg
    39661 aagatgccac tcaattgcgg cacatttgcc tgacagatgt ggcacttccc gctgatgatg
    39721 gagtgagttc atttactcaa ctgaaaaatc aacaacctgc caccttgtgc tatgcaccgc
    39781 cgctatcgac tgacgatacg gcggaaattc tcttcacctc cggcaccacc tcccgaccga
    39841 aaggtgtggt gattacccat tacaacctgc gcttcgctgg atattactcc gcctggcagt
    39901 gtgcactgcg tgacgatgac gtctacatga cggtaatgcc tgcgtttcat atcgattgcc
    39961 agtgtactgc ggcgatggcg gcgttttctg ccggggccac ctttgtgctg gtcgagaaat
    40021 acagcgcccg cgccttctgg ggacaggcgc agaagtaccg cgccaccatt accgaatgta
    40081 ttccgatgat gatccgtacg ttgatggtgc agccgccttc agcgaacgat cggcaacacc
    40141 gcctgcggga agtgatgttt tatctcaact tgtcggagca ggaaaaagac gcattttgtg
    40201 aacgcttcgg cgttcgcttg ctgacgtctt atgggatgac ggaaaccatt gtgggcatta
    40261 tcggcgatcg ccctagcgat aaacgacgct ggccgtcgat tggtcgggcg gggttttgct
    40321 acgaagcgga gatccgcgac gatcacaatc gcccgctccc ggcaggtgag atcggtgaaa
    40381 tctgtattaa aggcgtacca gggaaaacca tcttcaaaga gtattttctc aacccgaaag
    40441 ccactgcgaa agtgctggaa gccgacggct ggctacatac cggcgatacc ggataccgcg
    40501 acgaagaagg ctttttttat ttcgtcgatc gccgctgcaa tatgatcaaa cgcggtggcg
    40561 agaatgtctc ctgcgtggag ctggaaaata tcatcgccac ccatccgaaa attcaggaca
    40621 tcgtggttgt gggtattaaa gattcgattc gcgatgaagc catcaaagca tttgtggtgc
    40681 tgaatgaagg tgaaacattg agcgaagagg aattttttcg cttctgcgaa caaaatatgg
    40741 cgaaatttaa agtgccctct tatctggaga tcagaaaaga tctgccacgt aattgctcgg
    40801 ggaaaataat tagaaagaat ctgaaataaa tgaaacgtca gggaactacc ctgccagcta
    40861 ataatcacgc cctaaaacaa tatgcatttt ttgcagggat gttgtcgtcc ctaaaaaagc
    40921 aaaaatggag aaaaggaatg agtgaatcat tacatctgac ccgcaatgga tcaattctgg
    40981 aaattaccct tgatcgccca aaagcgaatg ctattgatgc aaaaaccagc tttgaaatgg
    41041 gcgaagtatt tctaaatttc cgtgacgatc cgcaattacg tgtcgccatt attaccggtg
    41101 ccggagagaa attcttttcc gcgggctggg atttaaaagc ggcagcagaa ggcgaagcac
    41161 cggatgctga ctttggcccg ggtggttttg cgggattaac cgaaattttc aatctcgaca
    41221 aaccagttat cgcagctgtg aacggctacg cctttggcgg cggtttcgaa ctggcgctgg
    41281 cggcagattt tattgtttgt gccgataacg ccagcttcgc cctgccggaa gccaaactgg
    41341 gcatcgttcc tgacagcggc ggtgtgctgc gtctgccgaa gatcctgccg cctaccatcg
    41401 tcaatgaaat ggtgatgacc ggcagacgaa tgggcgcaga agaggcgctg cgttggggga
    41461 tagtcaaccg cgtggttagc caggcggaac tgatggataa cgcccgcgaa ctggctcagc
    41521 agctggttaa cagcgccccg ctggcgattg cggcgctgaa agagatctac cgcaccacca
    41581 gcgaaatgcc ggtagaagaa gcgtatcgct atattcgcag cggcgtgttg aaacactatc
    41641 catcggttct gcattcggaa gatgccattg aagggccgct ggcgtttgcc gagaagcgcg
    41701 atccggtgtg gaaaggacgt taacaaccgt gagctattac gcctttgagg ggttaattcc
    41761 ggtggttcac ccgacggcgt ttgtccatcc cagtgccgtc ttgattggcg atgtgattgt
    41821 gggagccggt gtctacatcg gcccactcgc ctcactgcgt ggtgactacg ggcggttgat
    41881 cgtgcaagcg ggagccaata ttcaggatgg ctgcattatg catggctact gcgacactga
    41941 caccatcgtt ggggaaaacg gccatatcgg gcatggagcg atcctgcatg gttgtgtgat
    42001 tggtcgcgat gcattggtcg ggatgaacag cgtaattatg gatggcgcgg tcattggcga
    42061 agagagcatt gttgccgcaa tgagctttgt caaagcaggc tttagtggcg agaaacgcca
    42121 gttgttgatg ggtacgccag cccgcgctgt acgcagtgtt agtgacgacg agttacactg
    42181 gaaacggttg aataccaaag agtatcagga tcttgtcggg cgctgtcatg catcgttaca
    42241 tgaaacgcag ccactgaggc aaatggagga aaatcgcccc cgtttgcagg ggacgacgga
    42301 tgtgacgcct aaacggtgat cttgccggat gcgacctggt tgctggataa gatgctacaa
    42361 catcgtatct ggcaagcagg acgcccggta tcaacgagat aaaattaacg acgcatactc
    42421 tttgacaaca tcatctgcca cttctgctcg cggttaagtt ccgtgagcgg aacggcagga
    42481 atacgcggtg tctttcttac gccgactaat ttttcccgac tgttattgcg caggcgcgcg
    42541 taaatttgct catcgatatc gaccacttta accagtcgct gacactggca tccccgccct
    42601 tccagcttat tagggatcat cttaacttcg cagcttattt ctgtgacttc cgacagaata
    42661 taagtcaggg tattaaccgc cttgctgtgt tcaatatcga aatgaataga gatctctctt
    42721 gctatcaccc accgattttc agccatcatc cattcggcga ttaacaagta gagtggtttt
    42781 tcaacatatc cttcacacat acgaactcca gtttcacaaa ttaaaagtct ggctatgact
    42841 cgttttgaag caattaaaca agaccatatt aaaattgtgg atatatcgat agtatgcaac
    42901 ttcaccgtcg ataagtgtga gctaaatcct gcttatgtca tcaaaaatat cgattcccca
    42961 aaagatttac ttaatgggca aaaaaaaacg gtcctcatca gagaaccgta ttagttattc
    43021 gatcaaatta gcggatgaaa aatatccgcc acgacacgct attatttgat ctgtgcgtgc
    43081 atttcctgca ccgaaattac tttttcagtc gcatcggcat tcagcgccat cgcggtagcg
    43141 aaaccaccgt tcagggtggt gtcataatgc actttatatt gcagcgcact gcgacggatc
    43201 acgcgggagt cttcaatcgc acgacggcct gaggtggtgt tgatgatgta ggtatattcg
    43261 ccattcttga tacggtcctg aatgtgcgga cggccttcat gcaccttgtt taccagacgc
    43321 ggattgatac ccgcttcgcc cagcacaatc gccgtgccgt gggtcgcatc cagctcgaag
    43381 ccctgtttca gcagttttgc cgccaggtcc accacgcgtt ctttatcgcc ttcgcgcacg
    43441 gaaagcagcg cacgaccgtg tttcttcatg gtggagttgc tgcccagctg cgctttggcg
    43501 aacgcttcag cgaaggtgcg gcccacgccc atgacttccc cggtagagcg catttctggc
    43561 cctaacagcg ggtcaacgcc tgggaattta ttgaacggca gcaccacttc tttcaccgag
    43621 tagtacggcg gaataacttc tttggttacg ccctgctcag ccagcgattt gccagccatc
    43681 acacgcgccg ccacttttgc cagcggtacg ccggtggctt tggagacgaa cggaacagta
    43741 cgcgccgcac gcgggtttac ttcaatcagg taaacttcgt tgtttttcac cgcaaactgc
    43801 acgttcatca gaccgcgcac ctgcaattcg aaggccagtt tctgcacctg ctggcgcatc
    43861 acatcctgaa tttcctgact taaggtgtac gctggcagcg aacatgcgga gtcaccggag
    43921 tgcacgcccg cctgctcgat gtgctccatg atgccgccaa tcagcaccat ttcgccgtcg
    43981 cagatggcat ccacgtcaac ttctactgcg tcatcaagga aatgatccag caacactggc
    44041 gcatcgttag acacgctaac cgccgtctgg aagtagcgac gcaggtcagc ttcgtcatag
    44101 acgatttcca tcgcccgacc gccgagaacg taagacggac gcaccaccag cgggtagcca
    44161 atctcttttg ccttctcaac tgccatttca atagcagtaa cggtggcgtt cgccggttgt
    44221 ttcagtttca gacggtcaac cgcatgctgg aagcgttcac ggtcttccgc acggtcgata
    44281 gcatccgggc tggtgccgat aaccggtacg ccagcggctt ccagtgcacg cgccagtttc
    44341 agcggggttt gaccgccgta ctgaacgata acgcctttcg gcttctcaat acgcacgatt
    44401 tccagcacat cttccagagt taccggctcg aagtagaggc gatcggaagt gtcgtagtcg
    44461 gtagagacgg tttccgggtt acagttaacc ataatggttt cgtaaccgtc ttcacgcagc
    44521 gccagcgagg cgtgtacgca gcagtagtcg aattcgatac cctgaccgat acggtttgga
    44581 ccgccgccaa gcaccatgat tttttcacgg tcggtagacg gattcgcttc gcactcttct
    44641 tcataagtgg agtacatgta agcggtgtcg gtggcaaact ctgccgcaca ggtatccacg
    44701 cgcttgtaga ccgggtgcag gtcatattgg tcacgcagct tacggatttc cgcttcgcgt
    44761 acgcccgcca gttttgccaa gcgcgcatcg gcaaagcctt tgcgtttcag ctggcgcagg
    44821 aattcagcgt tcaggccagt gatgcccact tccgccactt tctcttccag acgcaccagt
    44881 tcttcaatct gtaccaggaa ccagcggtca atgttagtca ggttgaagac gccgtccaca
    44941 gacaggcccg cgcggaaagc atcggcgatg taccagatac gctctgcgcc tgcgtctttc
    45001 agttcgcgac ggattttggt taacgcttcc gggtcatcca ggctcacttt cgggtcgaag
    45061 ccagttgcgc ccacttccag gccgcgcagc gctttttgca gggattcctg ctgcgtgcga
    45121 ccaatcgcca tcacttcgcc aaccgatttc atctgagtgg tcagacggtc gttagcaccg
    45181 gcgaattttt cgaagttgaa gcgaggaatt ttagtaacca catagtcgat ggacggctcg
    45241 aaggaggccg gagtacgtcc gccagtgatg tcgttcatca gttcgtctag ggtgtaaccc
    45301 accgccagtt tcgccgccac tttagcaatc gggaaaccgg tcgctttcga cgccagcgcc
    45361 gaagaacggg acacgcgtgg gttcatttcg ataacaatca ggcgaccgtt tttcgggttc
    45421 accgcaaact ggacattgga accaccggtt tcaacgccga tttcacgcag caccgccatc
    45481 gaggcgttac gcatgatttg atattctttg tcagtcagcg tttgggctgg cgcgacagtg
    45541 atggagtcac cggtgtggat gcccatcgca tcaaagtttt cgatagagca gacgatgatg
    45601 cagttgtcgt ttttatcacg caccacttcc atctcgtact ctttccagcc gatcagcgac
    45661 tcatcaatca gcaactcttt ggtcggagag agatccagac cgcgggcgca aatttcttca
    45721 aactcttcac ggttataagc gataccgccg ccgctaccgc ccatggtaaa ggatgggcga
    45781 ataatgcacg ggaagcccac gtcagcggca acagccagcg cttcttccat cgtgtgtgcg
    45841 ataccggaac gcgcggtttc cagaccaatt ttcttcatcg ctacgtcgaa acgacggcgg
    45901 tcttctgctt tatcaatcgc atcggcagtg gcaccaatca tggtgacacc gaactcttcc
    45961 aacacgccct gacgttccag ctccagcgcg cagttcagcg ccgtctgacc gcccatggtt
    46021 ggcagcaccg cgtccgggcg ctctttttca ataatcttgc gtaccacttc ccagtgaatc
    46081 ggctcgatgt aggtcgcatc ggccatttcc gggtcggtca tgatggtcgc cgggttggag
    46141 ttcaccagaa tgacgcggta accctcttcg cgcagggctt tacatgcttg cgcgccagag
    46201 tagtcaaact cacacgcctg accgataaca atcgggcccg cacccagaat caggatactt
    46261 tttatatctg tacgttttgg catggctctt ttactcctga ttacttagcg gttttacggt
    46321 actgctcaat taacttgata aagtggtcga acaacggcgc ggcgtcgtgt ggaccagggc
    46381 tggcttcagg gtgaccctgg aagctgaacg ccggtttatc gatacgatga atgccctgta
    46441 acgtaccgtc gaacagggat ttatgcgtga cacgcaggtt tgcaggtaat gttgcttcat
    46501 ccaccgcaaa accgtggttc tgggcggtga tcatcaccac gtttttctcc acatctttaa
    46561 ccggatggtt gccgccgtgg tgaccaaatt tcattttgac agtcttcgca ccgctcgcca
    46621 gcgccagcag ctgatgaccg agacagatgc cgaataccgg aatatcggtt tcgaggaact
    46681 tctggatggc ggtaatggcg taatcgcacg gggccgggtc gccaggaccg ttggagagga
    46741 agatgccgtc tggattcatt ttcagcacat cttccgcaga agtttgcgcc ggaacgatgg
    46801 tcaggcgaca gcctctatcc accagcatcc gcaggatgtt gcgcttggca ccaaaatcat
    46861 aagccacgac gtggaacggc agctcgtctt cttttttcgc ttctggcagg ccgccggtca
    46921 acgtccagct cccttgtgtc cagctatagg cttctgcggt ggtcacttct tttgccagat
    46981 ccatgccgtt cagacctggg aacgcgcggg ctttttctaa cgccagcgcc gcatccgggt
    47041 tatcgcctgc gataatgcag ccattctgtg cgcctttctc gcgcagtaaa cgcgtcagct
    47101 tacgggtatc gatatcggca atcgccacga tgttatggcg cttcaggtaa gaagagaggt
    47161 cttcggtatt acggaagttg ctggcaatca gcggcaggtc gcgaatcacc agaccttgtg
    47221 catgtacctg agaagattct tcatcggcgt cattagtgcc gacattgcca atatggggat
    47281 aagtaagagt aacgatttga cgagaatagg aaggatcagt gaggatttct tgataaccgg
    47341 tcattgaagt attgaaaacg acttccccaa ccgccgaacc tgttgcccct atggcccgac
    47401 cgtgaaactg ggttccgtct tccagaacca atagcgctga cttaatcaaa acacctccat
    47461 agaatattca ctcactttat ttgcatatta attcacagtg atgacgtaaa tcaatgcaaa
    47521 tctgcttacc gacgaatttc tggcaaacgg cggcattctg gagatatggg cgctaaaagt
    47581 caacttaatg gcaggatttt ttatattatt ttgtggctca tgcatgtttt acaggcatta
    47641 agcgacaaaa acatcatcta acctgttctg gaaaacgctt gcgcagcaag aagattgcat
    47701 gaaataaaaa cgagcaggaa aaagtggacc aaatggtcaa aatttacatt aaaacaacaa
    47761 aaatagaaga cgaaacatgt aatttttggc agttatagaa aatttaaaag cataacacca
    47821 aaaataaaag ggctaaaaat taaagccctt tataattaat aaattaaatg tgttactttt
    47881 gcatcataat aaacattttg tggttacaaa ttattgagat caagcacatc tcgcatatca
    47941 aaaagaccac tttccttacc actcaaccac aaagccgatc ttaccgcacc gttagcaaat
    48001 gtcatgcggc tggacgcctt atgggtgatc tccagacgct cgccaatatc ggcaaacatc
    48061 gcggtatgtt cgccaacgat gtcacctgca cgcacggtgg caaaaccaat ggtaccaggc
    48121 acacgttcac cggtatggcc ttcacgactg tagaccgcgc aatctttcag atctttatca
    48181 agggcgtggg cgatcgcctc tcccattgcc agtgcggtgc ctgacggcgc atcaacttta
    48241 tgtctatgat gtgcttcaat aatttcgata tcggtgtagt cacccatcac tttggctgct
    48301 ttctccagca gcttaagcat gacgttaacg ccaacgctaa aattggcggc aaagacaatc
    48361 gcaatatcgg cggcggcgtc acgaattgcc tgtttaccgg cttcgtcaaa ccccgtagtg
    48421 ccgatcacca tccctttgcc atgctgccga caaaaagcga gatggttcag cgtaccttcc
    48481 ggacgggtaa aatcgataaa cacatcaaaa tcatctttta tcgcatcaag actgctttgc
    48541 acggtaacgc ctgttttccc tgcaccagcc agctcaccgg cgtcgctgcc cagtaaagaa
    48601 gatccttcgc gctccagcgc cgcgcccagc tgcacgcctt ctaatgccag tgccgcctga
    48661 atcaactggc ggcccatacg ccccccggct cccgcgatgg caacgcggat gtttgcatca
    48721 tgcatagcta ttctcttttg ttaatttgca tagaccgttt tcagagtaac cagcgcatcg
    48781 ctacgccgcc agccaaaaac gctgataatt agaaattaat gataaacagg gagggatatc
    48841 agtgaaaggc atgagtcgat gaatgactgc atgccgataa catgtgacag gttacggcac
    48901 cagagccagc acctcagcca cccactgttg gaagcctttt acattcagat ccaatgccac
    48961 ctgtacattg gctggtttgc ccaggcaacc gtcgatatca accaccgtcg tgcctgaggt
    49021 aaattcgcct tgagtttcta ccgcgacaaa acacggcttg agggtgaata agtccgggcg
    49081 caccagccag gcgatggcgc agagatcgtg cattcgcaga ccgctttgca tactgccgct
    49141 acggtagtgg ctaaacaggg cgtggagcat ttttccggtg cgattcaact ccggtaaagt
    49201 agcgagatag tcaggggtta atattgcctg attggtgaca tccagaccgc acatgacaat
    49261 ttcgatgccg ctacggaaga cacaggcagc cgcttctgga tcggcagcga tattaaactc
    49321 ggcatttggt gtacagttgc cgcgaccagc agaaccgccc atgatcacca gacgacgaat
    49381 atacggttta cattccgggc attgtaaaag taacagcgca atattggtta acgggccgat
    49441 ggccaccagg gtaacgggct ctggtgcacg catcagggca tcccgaatcg ccagaaacgc
    49501 cggtatcccg agtggctttc ggttgtgctc aacaaagtcg tagccagcca ttcccgattc
    49561 gccgtgcaca gatgctgcat cacgcggtgc gcgtaccagc ggcacagcgg ctccctgggc
    49621 gagcggaatc tccgcattcc agaaatgcag cagttgcagg gcattgcggg tagttttctc
    49681 aaccgagaca ttacccgcga cggtggtcat cagttgcagg tcgagttcgg gggcaaaaat
    49741 cgcggcggca atggcgacgg catcgtcaat gccggggtca gtatcgagga agataggtaa
    49801 acgcatgttt tcttcataaa aaatgccggt aacaagaccg gcattttcgc ataacttagg
    49861 ctgctaatga cttaatcgac ttcacgaata tcgacacgca gctctttcgg cacttcgaaa
    49921 acaatatttt cttcacggcc ttccagcgga atggcttcac caccacccaa ctgctgcaaa
    49981 cgtgccacca cattctgcac cagaatatcc ggagccgatg cgcccgcagt cacgccgacg
    50041 catttaacct ctttcaccca ctcttcctgg atatctttcg catcgtcaat caaaaacgcg
    50101 tgtttgccca tacgctgggc cagctccgcc agacggttgg agttggagga gtttttcgaa
    50161 ccgaccacca acacaacttc cgcctgttct gccagggcgc gtaccgcttc ctgacggtta
    50221 gtcgtggcgt agcagatgtc atctttgcgc ggaccgacaa ttttcgggaa gcgtttacgc
    50281 agcgcgtcga tcacatcaga cgtatcatcc accgacagcg tggtttgggt cataaaggag
    50341 agcttctctt cgtttttgac cgtcagtttc cacacatcgt ctggcgattc gaccagatac
    50401 attccccctt cggggttgct gtactgcccc atcgtccctt ccacttccgg gtgcccggcg
    50461 tggccgatga gaatagattc ttcgccacgg cgactggcgc gggcaacttc catatgcact
    50521 ttggtcacca gcggacaggt ggcatcaaac accgtcaaat cgcggctttt cgcttcgtta
    50581 cgaaccgcct gagaaacacc gtgtgcggag aaaatcagga tcgcgccgtc cggcacttcg
    50641 ctaatctgct caataaagat agccccacgc tcgcgcaagc tatcgaccac gtagcggtta
    50701 tgcaccactt cgtgacggac atatatcggt gcgccgtaaa tggccagcgc gttttcaaca
    50761 atgctgatag cgcggtctac cccggcacaa aaaccacgcg ggttggccaa caggatctgc
    50821 atgttacgcc tccagtgccg gatcgatttc cagcacttca atatcaaaat gaacggtctg
    50881 cccggccagc ggatggttga aatcaacggt aatggagtcg ccgttaattt cgcggatcac
    50941 accaggcatc tcactgccat ccattgcggt aaaaagcatg attgcgccaa tttctggctc
    51001 gcctgcatcc ataaattcac ggcgggagaa gtactgaatc aggtccggtg acggcacgcc
    51061 aaacgccgca tctggctcca gcgagaaggt ggttttgtcg cccactttca gccccagcag
    51121 gtgttgctcc agcccttcag aaagagaagc atcgcccagg cggaacagcg ccggtttacc
    51181 gttgttgcga gtagactcgg cggtggtgcc atcgtcgagt tttagcgtga agtgcaccag
    51241 gacggcgcta ttgctctgta cagattcaga catgaaggtt tgctcttatt tttgaggatt
    51301 tttgccggat gcggcctacg atttcgtagc aatctgtagg ccgcatccgg cagggtttat
    51361 tattgttttt tcgctttgga aggtaaaaac ccttccagca cgatcagtgc cgcaccgaca
    51421 caaatggcag tatcggcaag gttgaaggtg gcgaagtgcc agtcgccgac gtagaagtcg
    51481 atcatatcga caacgaagcc gtgccacagg cggtcgaaca ggttgcccag cgcgccgcca
    51541 ataatcagcg cgtaagcgat attgtttagc ttctgcgtgg ccttcgagcg atacatcatc
    51601 actgccagga gcacgctaat accaatcgca ataccggcaa agaaccaacg ctgccagccg
    51661 ccgctatcgg caaggaaact aaacgccgcg ccatagttac gcgcataatg cagattaagc
    51721 gacgggaaca gcgggaccgt atcccccaga gcaaagttct ggaggatcag gtatttgctg
    51781 cccagatcga taatcagcac gactaccacc agccacagcc agcgtagccc tgttgaacag
    51841 atcgattgac tcatcaggca aacttacgtt tttcaccgtc accggcgacg ttgctgacac
    51901 agcggccgca gatttctgcg tgttccgcca ccttgccgac atcctgggtg tagtgccagc
    51961 agcgtgggca cttctcacct tcggctttac tcaacgcgac tttcagccct ttgagtactt
    52021 cgctctgctg agcatcagca ggtgcgtcgt tatagtctgc aacggtagcg ccggaggtca
    52081 acaggacaaa tcgtaattca tcgcccagcg cggtcagttt cgccgccagt tccggttctg
    52141 catacaaggt tactgccgct tccagcgagc cacccacttt cttgtcggca cgcgcttgct
    52201 caatgacttt gttcacttcg ccacgcactt tcaacagctc gtcccagaac gcatcgttca
    52261 ttgcttcact gtctgccaga ccaaacaggc cttcgtacca ctcaccggtg aagacgtatt
    52321 tttcacgttc gcccggcaga tagccccaca cttcatcagc ggtgaaggag aggattggtg
    52381 ccatccagcg caccagcgct tcagcgatgt gatacagcgc agtctggcag ctacgacgcg
    52441 ccacgctatc cgctttggcg gtgtactgac ggtctttgat gatgtcgagg tagaaggaac
    52501 ccatctcaac ggagcagaag cgcatcagac gctgtaccac ttcgtggaaa tcgtatgctt
    52561 cgtacgcctt gaggatgtct tcctgtgccg ctttcgcaca acctacggcc cagcgatcca
    52621 gtaccaccat ctcttccggt ttcaccatat cttttgctgg atcaaaacca ttcaggtttg
    52681 ccagcaggaa gcgcgcggtg ttacggatac gacgatagct atcggcagca cgtttcagga
    52741 tctcgtcaga aacggccatt tcaccggtgt agtcggttga tgccacccac agacgcagaa
    52801 tatccgcgcc cagtttgttc atcacatcct gcggcgaaac ggtgttgccg atggatttag
    52861 acatcttgcg accctgacca tccacggtaa agccgtgggt cagtacctga cgatacggcg
    52921 ctttgccttt catcgcggtg gagatcatta gggaagacat gaaccaacca cggtgctggt
    52981 cagaaccttc cagatacatg tccgctgcgt gaccggcaaa ttccggacgc acgtcaacaa
    53041 cagaagagtg ggtagatccg gagtcaaacc atacgtcgag agtatccggc actttcacat
    53101 actggtcagc ttcgtcgccg aggatctctt tcgcatcgag atcccaccac gcctggatgc
    53161 cgtcaacttc aacgcgttta gccacttctt ccatcagttc gagggtacgc ggatgcagct
    53221 cttccgtgtc tttgtgcacg aacagtgaca tcggtacgcc ccaggtgcgc tgacgggaga
    53281 tacaccagtc aggacggtta gcaaccatcg actcgatacg cgcctggccc cagtccggga
    53341 tccactgcac gcctttgatc tctttcagtg actgcgcacg cagacctttc tgatccatgc
    53401 tgacgaacca ctgcggtgtc gcgcggaaga tgatcggcgt tttgtgacgc cagcagcacg
    53461 gatagctgtg ctgcattttc tcaacgtgca gcagcgcgcc tttttcctgc agcagcgcaa
    53521 cgacgatgtc gttcgctttg aagacgttca cgccatccag cgtcggataa gtgcccggca
    53581 gataagtgcc gtccgggcca accgggttag cggtttccag gccgtatttc tgaccgatca
    53641 catagtcgtc cgggccgtgg ccaggcgcgg tgtgaacggc accggtaccg gcatccaggg
    53701 taacgtgatc gccgaggatt gccggaacgt cgaagcccat aaacggatgg gtaaagcgca
    53761 gcagctcaag ctctgcacct tttaccgtgc cgagaatggt gtaatcggtc acgccgatac
    53821 gctgcattac gctttcaacc agatctttcg ccagaatcgc ggcctgaccg tcgatctgca
    53881 ccagcgcata gtcgaaatca ggtgcaatag agattgcgcg gttcgcaggc aaagtccacg
    53941 gcgtagtggt ccagatcacc agcgagattg ggccgttaac gttgcttacg ccaaatttcg
    54001 ctttcagcgc atcctgatcg accgcctgga aagcgacgtc aatggacgga gaagttttgt
    54061 cgtaatactc aacttccgct tctgccagtg cagaacggca gtctacgcac cagtgcaccg
    54121 gcttcgcgcc tttgtgcagg tgaccattgc cgatgatttt gcccagcgcg cggatgatgt
    54181 tggcttcagt tttgaagtcc atagtcagat acgggtgcga ccagtcgccc agcacgccca
    54241 gacggataaa gtctttgcgt tgaccgtcaa cctgggtcgc cgcgtattcg cggcacttgg
    54301 cgcggaactc ggcggcggtg aatttctcac ccggcttacc gtattcttgc tctactttca
    54361 gctcgatcgg cagaccgtgg cagtcccagc caggcacata cggcgagtca taaccggaaa
    54421 gccctttgga cttcacgata atgtctttca gaatcttgtt aaccgagtga ccaatatgaa
    54481 tgctgccatt cgcataagga gggccatcat gcagaatgaa ggtttttttg ccttttttag
    54541 ccgcacggat gatgccgtac agatcatcat cagtccaacg cgccagcatt cccggttcgc
    54601 gcttggcgag atcgccacgc atcgggaacc ctgtttccgg caaattcagg gttgatttat
    54661 agtcactcat cagattctcg gttccgtgtt tcggtttgat tacataacag gcttaagccg
    54721 gttttgttag cccaaaaaat tcgcgggcgg ttaattcatc acgcgcaatc tgcgctttca
    54781 gttcgtccag cgacgcaaat cgctgctcat tgcgtatttt tttacgcagc actacttgta
    54841 tatggcgacc gtaaaggtcc attgcaacat ctaacaaatg cacttccagc tgctggcgaa
    54901 taccggcaac cgttgggcgt gttccgatgt ttgccacgcc gggtaacggc ttttcaccga
    54961 ggcccagcac ttctaccgca taaacccctt tcaccgggga aacctgacgg cgtagcggta
    55021 cattcgccgt cgggaaacct atagtgcgcc ctaattcatc accgtggact acacgcccgg
    55081 agatggcaaa cgggtgcccc agtaaacttt ctgccagagc cagattgtca tcagccagcg
    55141 cctgacgcac ggcggtgctg ctgatacgca caccaccttc gcaaaaagtt tgcgtactgg
    55201 tgatatcgaa gccgtattcc atgccagctt tctgtaataa caagaaatcg ccttcacgac
    55261 cagcgccaaa gcggaaatca tcacctacgg caagaaattt tacgcgcaaa tgcttcacca
    55321 aaagatcgct gatgaaattt tgcgcggtta acgccgcaaa acgcctgtcg aaacgcacgc
    55381 acagcacgta atcaacgcca cactctgcaa ggtaacgcag tttttcccgc agccgggtca
    55441 gtcttgccgg ggctttatcg gtagcaaaca gttccagtgg ttgaggttca aaaagcatca
    55501 ccatcaccgg taagttgcgc ttgcgccctt cttcctgcaa gccctgtaac agcgcgcgat
    55561 gaccgcgatg cacgccgtcg aaattaccaa tagtcagcac acacccttct tgcggggcct
    55621 ggctgagatt atgtatgccg cgtatcagct tcatgtctgg ctcaaaacag tgaaaatcgt
    55681 ccgagtatac cttgtacagc ggtcaaggtt aaccggcgat tgagtaccga aatcagaaag
    55741 aagtggcttt tcattgccat ggcgcaaatc acgggaagaa actgaccgcc tgctgcaatt
    55801 tttatcgcgg aaaagctgta ttcacacccc gcaagctggt agaatcctgc gccatcacta
    55861 cgtaacgagt gccggcacat taacggcgct tatttgcaca aatccattga caaaagaagg
    55921 ctaaaagggc atattcctcg gcctttgaat tgtccatata gaacacattt gggagttgga
    55981 ccttggctaa tatcaaatca gctaagaagc gcgccattca gtctgaaaag gctcgtaagc
    56041 acaacgcaag ccgtcgctct atgatgcgta ctttcatcaa gaaagtatac gcagctatcg
    56101 aagctggcga caaagctgct gcacagaaag catttaacga aatgcaaccg atcgtggacc
    56161 gtcaggctgc taaaggtctg atccacaaaa acaaagctgc acgtcataag gctaacctga
    56221 ctgcacagat caacaaactg gcttaatcgc caatttgctg aagctttgtg aaaaagcccg
    56281 cgcaagcggg tttttttatg cctgctgctt ttgtgatgcg tgagagaaca cactgccggt
    56341 tgccagatag tcaaaagaga catgccgttg tactatctga actcttcgtg gcttaagaat
    56401 cgtagaacca tcggctgata cattctgaac aagaatatct actcaatctc cagactcccc
    56461 cacaaagaat atggatattg tgatacacat tgaggtatta aacttaagac cactctattt
    56521 ttgaattaaa ttcctatata ctttaaccat tcataaatat ctcctcatta gac
//
LOCUS       DADMGC010000010        56337 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.010, whole genome shotgun sequence.
ACCESSION   DADMGC010000010 DADMGC010000000
VERSION     DADMGC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.010"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..358)
                     /locus_tag="JNZ73_000611"
     CDS             complement(<1..358)
                     /locus_tag="JNZ73_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY8731006.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREH"
     gene            complement(429..2543)
                     /gene="fusA"
                     /locus_tag="JNZ73_000612"
     CDS             complement(429..2543)
                     /gene="fusA"
                     /locus_tag="JNZ73_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAY8731007.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2640..3110)
                     /gene="rpsG"
                     /locus_tag="JNZ73_000613"
     CDS             complement(2640..3110)
                     /gene="rpsG"
                     /locus_tag="JNZ73_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAY8731008.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3207..3581)
                     /gene="rpsL"
                     /locus_tag="JNZ73_000614"
     CDS             complement(3207..3581)
                     /gene="rpsL"
                     /locus_tag="JNZ73_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAY8731009.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3707..3994)
                     /gene="tusB"
                     /locus_tag="JNZ73_000615"
     CDS             complement(3707..3994)
                     /gene="tusB"
                     /locus_tag="JNZ73_000615"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HAY8731010.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4002..4361)
                     /gene="tusC"
                     /locus_tag="JNZ73_000616"
     CDS             complement(4002..4361)
                     /gene="tusC"
                     /locus_tag="JNZ73_000616"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HAY8731011.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4361..4747)
                     /gene="tusD"
                     /locus_tag="JNZ73_000617"
     CDS             complement(4361..4747)
                     /gene="tusD"
                     /locus_tag="JNZ73_000617"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HAY8731012.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4747..5469)
                     /locus_tag="JNZ73_000618"
     CDS             complement(4747..5469)
                     /locus_tag="JNZ73_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8731013.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5636..6448)
                     /gene="fkpA"
                     /locus_tag="JNZ73_000619"
     CDS             complement(5636..6448)
                     /gene="fkpA"
                     /locus_tag="JNZ73_000619"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY8731014.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6669..6887
                     /locus_tag="JNZ73_000620"
     CDS             6669..6887
                     /locus_tag="JNZ73_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HAY8731015.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6936..7526)
                     /gene="slyD"
                     /locus_tag="JNZ73_000621"
     CDS             complement(6936..7526)
                     /gene="slyD"
                     /locus_tag="JNZ73_000621"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY8731016.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETEQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7621..7821)
                     /locus_tag="JNZ73_000622"
     CDS             complement(7621..7821)
                     /locus_tag="JNZ73_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HAY8731017.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7831..9636)
                     /gene="kefB"
                     /locus_tag="JNZ73_000623"
     CDS             complement(7831..9636)
                     /gene="kefB"
                     /locus_tag="JNZ73_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HAY8731018.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGVAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARVRGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9636..10187)
                     /gene="kefG"
                     /locus_tag="JNZ73_000624"
     CDS             complement(9636..10187)
                     /gene="kefG"
                     /locus_tag="JNZ73_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HAY8731019.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSSIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10318..12231
                     /locus_tag="JNZ73_000625"
     CDS             10318..12231
                     /locus_tag="JNZ73_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8731020.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIQ
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQAVTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12231..13253
                     /locus_tag="JNZ73_000626"
     CDS             12231..13253
                     /locus_tag="JNZ73_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY8731021.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13247..13465
                     /locus_tag="JNZ73_000627"
     CDS             13247..13465
                     /locus_tag="JNZ73_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HAY8731022.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13519..14388
                     /locus_tag="JNZ73_000628"
     CDS             13519..14388
                     /locus_tag="JNZ73_000628"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HAY8731023.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     LLVQRLMEGKKIE"
     gene            complement(14443..14847)
                     /locus_tag="JNZ73_000629"
     CDS             complement(14443..14847)
                     /locus_tag="JNZ73_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HAY8731024.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15149..15781
                     /gene="crp"
                     /locus_tag="JNZ73_000630"
     CDS             15149..15781
                     /gene="crp"
                     /locus_tag="JNZ73_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HAY8731025.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15832..17922
                     /locus_tag="JNZ73_000631"
     CDS             15832..17922
                     /locus_tag="JNZ73_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731026.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17989..19209)
                     /gene="argD/dapC"
                     /locus_tag="JNZ73_000632"
     CDS             complement(17989..19209)
                     /gene="argD/dapC"
                     /locus_tag="JNZ73_000632"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HAY8731027.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPQPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQVLLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19295..19858)
                     /gene="pabA"
                     /locus_tag="JNZ73_000633"
     CDS             complement(19295..19858)
                     /gene="pabA"
                     /locus_tag="JNZ73_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HAY8731028.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19890..20492)
                     /locus_tag="JNZ73_000634"
     CDS             complement(19890..20492)
                     /locus_tag="JNZ73_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HAY8731029.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEIDVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20482..20649)
                     /locus_tag="JNZ73_000635"
     CDS             complement(20482..20649)
                     /locus_tag="JNZ73_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HAY8731030.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20754..21326)
                     /gene="ppiA"
                     /locus_tag="JNZ73_000636"
                     /gene_synonym="rotA"
     CDS             complement(20754..21326)
                     /gene="ppiA"
                     /locus_tag="JNZ73_000636"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HAY8731031.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21597..22778
                     /gene="tsgA"
                     /locus_tag="JNZ73_000637"
     CDS             21597..22778
                     /gene="tsgA"
                     /locus_tag="JNZ73_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HAY8731032.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFSCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23040..25583
                     /locus_tag="JNZ73_000638"
     CDS             23040..25583
                     /locus_tag="JNZ73_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HAY8731033.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIARKFNLYTKITGSQRLAMFGAQKGDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25580..25906
                     /gene="nirD"
                     /locus_tag="JNZ73_000639"
     CDS             25580..25906
                     /gene="nirD"
                     /locus_tag="JNZ73_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HAY8731034.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26032..26838
                     /gene="nirC"
                     /locus_tag="JNZ73_000640"
     CDS             26032..26838
                     /gene="nirC"
                     /locus_tag="JNZ73_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HAY8731035.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26857..28230
                     /gene="cobA"
                     /locus_tag="JNZ73_000641"
     CDS             26857..28230
                     /gene="cobA"
                     /locus_tag="JNZ73_000641"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY8731036.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28477..28644
                     /locus_tag="JNZ73_000642"
     CDS             28477..28644
                     /locus_tag="JNZ73_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HAY8731037.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28939..30276
                     /gene="frlA"
                     /locus_tag="JNZ73_000643"
                     /pseudo
     CDS             28939..30276
                     /gene="frlA"
                     /locus_tag="JNZ73_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30297..31319
                     /gene="frlB"
                     /locus_tag="JNZ73_000644"
     CDS             30297..31319
                     /gene="frlB"
                     /locus_tag="JNZ73_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HAY8731038.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITLLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31369..32199
                     /gene="frlC"
                     /locus_tag="JNZ73_000645"
     CDS             31369..32199
                     /gene="frlC"
                     /locus_tag="JNZ73_000645"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HAY8731039.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIYMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32196..32981
                     /gene="frlD"
                     /locus_tag="JNZ73_000646"
     CDS             32196..32981
                     /gene="frlD"
                     /locus_tag="JNZ73_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAY8731040.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            33081..33812
                     /gene="phoB"
                     /locus_tag="JNZ73_000647"
     CDS             33081..33812
                     /gene="phoB"
                     /locus_tag="JNZ73_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HAY8731041.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33972..35057)
                     /locus_tag="JNZ73_000648"
     CDS             complement(33972..35057)
                     /locus_tag="JNZ73_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY8731042.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLLALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35069..36373)
                     /locus_tag="JNZ73_000649"
     CDS             complement(35069..36373)
                     /locus_tag="JNZ73_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731043.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTWLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEMALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36385..36676)
                     /locus_tag="JNZ73_000650"
                     /pseudo
     CDS             complement(36385..36676)
                     /locus_tag="JNZ73_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
     gene            complement(36687..37565)
                     /locus_tag="JNZ73_000651"
     CDS             complement(36687..37565)
                     /locus_tag="JNZ73_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HAY8731044.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLCQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37562..38788)
                     /locus_tag="JNZ73_000652"
     CDS             complement(37562..38788)
                     /locus_tag="JNZ73_000652"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY8731045.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38789..39952)
                     /locus_tag="JNZ73_000653"
     CDS             complement(38789..39952)
                     /locus_tag="JNZ73_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HAY8731046.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLKA"
     gene            complement(40036..40398)
                     /locus_tag="JNZ73_000654"
     CDS             complement(40036..40398)
                     /locus_tag="JNZ73_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY8731047.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40415..41320)
                     /locus_tag="JNZ73_000655"
     CDS             complement(40415..41320)
                     /locus_tag="JNZ73_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731048.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLLATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41590..42594)
                     /gene="trpS"
                     /locus_tag="JNZ73_000656"
     CDS             complement(41590..42594)
                     /gene="trpS"
                     /locus_tag="JNZ73_000656"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAY8731049.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42587..43345)
                     /gene="gph"
                     /locus_tag="JNZ73_000657"
     CDS             complement(42587..43345)
                     /gene="gph"
                     /locus_tag="JNZ73_000657"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HAY8731050.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43338..44015)
                     /gene="rpe"
                     /locus_tag="JNZ73_000658"
     CDS             complement(43338..44015)
                     /gene="rpe"
                     /locus_tag="JNZ73_000658"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAY8731051.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(44033..44869)
                     /gene="dam"
                     /locus_tag="JNZ73_000659"
     CDS             complement(44033..44869)
                     /gene="dam"
                     /locus_tag="JNZ73_000659"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY8731052.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44976..46262)
                     /gene="damX"
                     /locus_tag="JNZ73_000660"
     CDS             complement(44976..46262)
                     /gene="damX"
                     /locus_tag="JNZ73_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HAY8731053.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46354..47442)
                     /gene="aroB"
                     /locus_tag="JNZ73_000661"
     CDS             complement(46354..47442)
                     /gene="aroB"
                     /locus_tag="JNZ73_000661"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAY8731054.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47499..48020)
                     /gene="aroK"
                     /locus_tag="JNZ73_000662"
     CDS             complement(47499..48020)
                     /gene="aroK"
                     /locus_tag="JNZ73_000662"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HAY8731055.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQKLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48421..49659)
                     /gene="hofQ"
                     /locus_tag="JNZ73_000663"
     CDS             complement(48421..49659)
                     /gene="hofQ"
                     /locus_tag="JNZ73_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HAY8731056.1"
                     /translation="MKQWIAALLLMLISGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49571..49975)
                     /gene="hofP"
                     /locus_tag="JNZ73_000664"
     CDS             complement(49571..49975)
                     /gene="hofP"
                     /locus_tag="JNZ73_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HAY8731057.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADIRRTGGKAAKSDADGG"
     gene            complement(49965..50405)
                     /gene="hofO"
                     /locus_tag="JNZ73_000665"
     CDS             complement(49965..50405)
                     /gene="hofO"
                     /locus_tag="JNZ73_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HAY8731058.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50389..50928)
                     /locus_tag="JNZ73_000666"
     CDS             complement(50389..50928)
                     /locus_tag="JNZ73_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HAY8731059.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50928..51707)
                     /gene="hofM"
                     /locus_tag="JNZ73_000667"
     CDS             complement(50928..51707)
                     /gene="hofM"
                     /locus_tag="JNZ73_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HAY8731060.1"
                     /translation="MTFKIWQIGLHLQQQEVAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51827..54379
                     /gene="mrcA"
                     /locus_tag="JNZ73_000668"
     CDS             51827..54379
                     /gene="mrcA"
                     /locus_tag="JNZ73_000668"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HAY8731061.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54544..55104)
                     /gene="nudE"
                     /locus_tag="JNZ73_000669"
     CDS             complement(54544..55104)
                     /gene="nudE"
                     /locus_tag="JNZ73_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HAY8731062.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55424..>56337
                     /gene="igaA"
                     /locus_tag="JNZ73_000670"
     CDS             55424..>56337
                     /gene="igaA"
                     /locus_tag="JNZ73_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HAY8731063.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQ"
BASE COUNT        13449 a        15353 c        14198 g        13337 t
ORIGIN      
        1 tgtgctcacg agtctgcggc atcgggccgt cagtcgcagc aactaccagg atcgcgccgt
       61 ccatctgagc agcaccggtg atcatgtttt taacatagtc ggcgtgcccc gggcagtcta
      121 cgtgtgcgta gtgacgggtc ggggtgtcat attcaacgtg agaagtgttg atggtgatac
      181 cacgagcttt ttcttccggc gcgttatcga tctggtcgaa tgcacgagca gcaccgccgt
      241 aggttttagc cagtacggtg gtgattgcag cggtcagagt agttttaccg tggtcaacgt
      301 ggccgatagt accaacgtta acgtgcggtt ttgtacgttc aaatttttct ttagacacgg
      361 ctatattcct tactatagtg ctctcccctt caggagagag cacgggactt tggtattaac
      421 ccttaggctt atttaccacg ggcttcaatt acggcctgag caacgttact cggcgcttca
      481 tcatacttca ggaattccat agtgtatgat gcacgacctt tggtcagaga acgcagctga
      541 gttgcgtatc cgaacatttc agacagcggt acttcagcgt ggatcttaac gccagtaact
      601 tcagattcct gacctttgag cataccacga cgacggctca agtcaccgat aacgtcaccg
      661 gtgttctctt ccggagtttc tacttcaacc ttcatgatcg gctcaagcag aactggtttc
      721 gctttcttaa agccttcttt aaaggcgata gaagcagcca gtttaaacgc cagttcagag
      781 gagtcaacgt catggtaaga accgaagtgc agacgaatac ccatgtctac taccgggtag
      841 cctgccagcg gacctgcttt cagctgttcc tggatacctt tatcaacggc cgggatgtat
      901 tcgccaggga ttacaccacc tttaatgtcg ttgatgaact cgtagccttt cgggtttgaa
      961 cccggctcca gcgggtacat gtcgataaca acatgaccat actgaccacg accaccagac
     1021 tgtttcgcgt gtttaccttc aacatcggta actttctggc ggatagtttc acggtaagca
     1081 acctgcggtt tacctacgtt cgcttcaacg ttgaattcac gcttcatacg gtcaacgatg
     1141 atgtcgaggt gcagttcgcc catacccgcg atgatggtct ggttagattc ttcgtcagtc
     1201 catacacgga aagacgggtc ttctttagcc agacggccca gagccagacc cattttttcc
     1261 tggtcagctt tggttttcgg ttcaactgcg atggagatta ccggctcagg gaattccata
     1321 cgttccagaa tgatcggcgc atccgggtca cacagggtgt caccagtggt tacgtctttc
     1381 agaccgatag cagcagcgat gtcgcccgcg cgaacttctt tgatctcttc acgtttgtta
     1441 gcgtgcatct gaacgatacg accgaaacgc tcacgtgcag ctttcacgga gttcagtacg
     1501 gtatcaccag agttaaccac accggagtaa acacggaaga aggtcaggtt accaacaaac
     1561 gggtcggtag cgattttgaa cgccagtgca gagaacggct cgtcatcact tgcgtgacgt
     1621 tcagccggag tgtctttacc gtcgtccagg ataccgttga tcgcaggtac gtcaaccggg
     1681 gatggcaggt aatcaattac cgcatccagc atcgcctgaa cacctttgtt cttgaacgca
     1741 gaaccacagg ttaccaggat gatttcgttg ttcagaacgc gctgacgcag agcacctttg
     1801 atttctgctt cagtcagttc ttcaccaccc aggtattttt ccatcagctc ttcagaagct
     1861 tcagctgcgg attcgatcag gttctggtgc cattcgttag ccagttcaac catgtctgcc
     1921 gggatatctt cgtattcgaa ggttacgccc tggtcagcgt cgttccagtt gatagctttc
     1981 attttcacca ggtcaacaac accggtgaaa tgttcttcag caccaatcgc cagctgcagc
     2041 ggaaccgggt tcgcgcccag acgggttttg atctggttaa caactttcag gaagttcgca
     2101 cccatgcggt ccattttgtt aacgaacgca atgcgcggaa ctttatattt gtttgcctga
     2161 cgccatacgg tttcagactg cggctgaaca ccaccaactg cgcagtaaac cattaccgca
     2221 ccatcgagaa cacgcatgga acgttctact tcgattgtga agtcaacgtg ccccggggtg
     2281 tcgatgatgt tgatgcgatg cggctcatac tgcttagcca taccagacca gaatgcagta
     2341 gtcgcagcgg aagtgatggt aataccacgt tcctgctcct gctccatcca gtccatggtt
     2401 gcagcgccgt catgaacttc accgatttta tggtttacac cggtgtagaa cagaatacgt
     2461 tcggtagtag tggttttacc ggcgtcgatg tgcgcactga taccgatgtt acggtagcgt
     2521 gcgatgggtg ttgtacgagc catttgtttc ctcgtttatc ttttaggcgt tcaatttaag
     2581 tagcccaaag cgggctgctt actggaagcg cccgcctggt gactaaaact ccgaagggat
     2641 taccaacggt agtgtgcgaa cgccttgttg gcttcggcca tacggtgaac gtcttcacgt
     2701 ttcttaactg cagtaccttt gttttctgca gcatcagaaa gttcgttcgc caggcgcaga
     2761 gccatggatt tatcaccgcg tttacgagca gcttcaacga tccaacgcat tgccagagca
     2821 ttacgacgaa ccggacggac ttcaactggt acctgataag tagaaccacc aacgcggcga
     2881 gacttaactt ctacagtcgg gcgcacgttt tcgagagcta cttcgaatgc ttccagttca
     2941 gatttaccag agcgctgagc cagggtctcc agcgcgctgt atacgataga ttcagcagta
     3001 gattttttac catctaccat caggatattt acaaatttag ccagcagttc tgatccgaac
     3061 ttcggatccg gcagaatttt acgctgacca atgacgcgac gacgtggcat ggaaatactc
     3121 cgttgttaat tcaggattgt ccaaaactct acgagtttag tttgacattt aagttaaaac
     3181 gtttggcctt acttaacgga gaaccattaa gccttaggac gcttcacgcc atacttggaa
     3241 cgagcctgct tacggtcttt aacgccggag cagtcaagcg caccacgtac ggtgtggtaa
     3301 cgaacacccg ggaggtcttt aacacgaccg ccacggatca ggatcacgga gtgctcctgc
     3361 aggttgtgac cttcaccacc gatgtaggaa gtcacttcga aaccgttagt cagacgaaca
     3421 cggcatactt tacgcagcgc ggagttcggt tttttaggag tggtagtata tacacgagta
     3481 catacgccac gtttttgcgg gcatgcttcc agcgcaggca cgttgctttt cgcaactttg
     3541 cgagcacgtg gtttgcgtac cagctggtta actgttgcca ttaaatagct cctggtttta
     3601 gcttttgctt cgtaaacacg taataaaacg tcctcacaca atatgaggac gccgaatttt
     3661 agggcgatgc cgaaaaggtg tcaagaaata tacaacgatc ccgccatcac caggccatct
     3721 ggctggagtg cttaaccgta agtctgacga aatcagtata gtcaatggga atgatgtcgt
     3781 tcaaaatttg aacagtcaaa ccgcgggcaa taaggtcttc gttcagggca tagaccttaa
     3841 tgggggcatt acgcagactt tcaaggtagc ggttaccgtc aactgcggca gttacgccat
     3901 cttgcaataa tagcagttcg tctccttcac tgagcagacg cagcagcgca gcaaaatccg
     3961 tcagccaggg tgaacgatgt aacgtgtgca gcataaacgc ctcaaaacct caaaataaca
     4021 tcgtagttgg cgagttcgcg gcgtaaggca tctgcttcga gcggcgtggc ttcgacaaca
     4081 aagggtgtct gcggatctaa cccgcgttcg cgcagtgaag ccgcacaaac ccagcactgt
     4141 tcaatgtcgt acagactcaa caatttaaaa gtggcaatgt aatcacgcgc cagcactgca
     4201 tcgggctttt gtcctgacag cagctgaaaa acgccatcag caataaagaa gacggccaga
     4261 tcgtcagtta atgcggaagt tgccagtaaa gcatctaaac cttcccgacc agcagctgta
     4321 ccatgaggtg cagtagaaaa aacaaacgca attcgtttca tcagaactgt accaccctgt
     4381 cacaggtcag cgaggcttcc gccagcgcgc caagtccgct taaggtaaat cccggctgaa
     4441 ggtttgacga agccagcccc agccttcctg cttcagtttc atcgacgata ccgcggcgta
     4501 acgctgccgc tacgcagata ttcagcgcca caccatgttg cgcattcaac tgttgccagc
     4561 cgcgcacaag gtcaaattcg tcgcttgccg gtgaggtcag ttggttagcg ttatagaccc
     4621 cttcccgata gaaaaagacg ctgcttaact tatggccttc tgctatcagc gcctgcgcaa
     4681 actgaaaagc actactcgcc tgttgcgtac cgtatgctgg cccggtcacc acgatggcaa
     4741 aacgcattac ttatcttgcc cctgaaaatc accgctcttg aactggcgga tgtagagata
     4801 gacagtgtgt ttggagatgt tcaggcggtc agcaacctgg ttgatcgcat ctttaatatc
     4861 gaagatccct ttctcgtaga gattcagcac gatctgacga tttttggcgt tattagaaac
     4921 attgcgatcg gcattcactt cttcgatggt gaactccagc gtttgggtaa ccagatcttc
     4981 aacagaagag gcaaagttga cgcttgaacc gacatccggg gtttctggcg gcacaaaagt
     5041 gctcataatc tgcgagaagg gaacatcaag attcatattg atgcacagca gaccaattac
     5101 acgctgttcg cggttacgaa tcgcgatagt cagggacttc attaatacgc cgcttttggc
     5161 gcgagtaaag tagcatttag aaacgctgct atccgctccc gtcatatcgt gcagcatacg
     5221 tagcgccagg tcagtaattg gcgaaccaat cttccggcct gtatgttcac cgttagcaat
     5281 gcgaatggct gaacatttta gatcctgcaa agagtgcaaa acgatttcac agtgggagcc
     5341 aataagcatc gctaacccgt ccaccaccgc ttcgtaggat ttcagaatat caaaatcggt
     5401 ctggtcgaaa ggacgttgat ccagtaaatc caactcactg gtttcgttgg ttaaaagcga
     5461 cctggacatg aaaaaaaaca ctccttttca ggagcctgtc gttaactttt cagggcaggc
     5521 tcattaatga tgcgggtaac taaattaata cagcggaggt tccgctttcc agcactaatt
     5581 atatccggcc tgtaataaaa aaaccgccgc ctggtcaggc ggcggttctt aatgcttatt
     5641 ttttagcaga atctgcggct ttcgcatcag cttccggctt tgcatcagcc ttcggcgctg
     5701 gtttcacatc cagcagctct acgtcaaaca ctagggtaga attcggtggg atccccggaa
     5761 cacccgcttt gccgtaagcc agttctggtg gaataaccag tttgatctta ccgcctttct
     5821 tgatgttctt cagaccttct gtccaacccg ggataacacc gtccagacgg aaagaaagcg
     5881 gttcaccacg ggtgtaagag ttgtcgaact ctttaccgtc gatcagcgta cctttgtagt
     5941 tcactacaac agtatcgctg tctttcggtg cttcgccttt accggcttct actacctgat
     6001 aaaccagacc agttgaagag gttttcacac ctttctcttt ggcaaatttc tcgcggtact
     6061 ctttaccttt tgcttcgtta tcagccgcgt ctttttccat cttcgcctga gcagaagact
     6121 tcacgcgagc ttcgaatgct tgcagagtct gttcgatctc ttggtcggag agtttgctct
     6181 tatcagcaaa tgcatcctga acaccagcga tcagctgatc tttatccagt ttgatgccca
     6241 gtttttcttg ttctttaaga gagttttcca tgtaacgacc cagcgaagca cccagtgcat
     6301 aagctgattt ctgatcgtca tttttgaacg ctgctttgct gtcagcagtt gtagcaggtt
     6361 ttgcagcttc agcagcaaaa gtgattggtg catgcagggc aacggccatt gtggtcgcca
     6421 gtagcgttac tttaaacagt gatttcatcc atatctccag gatcggggca tctcacccca
     6481 gggttaacta ttatcagaag ggtactataa agcgttgtcg aacaaatcta catacagaca
     6541 cgccctatta tcatctattt tcagactctt tttgtttaaa ttagtttcga tgaccgcgaa
     6601 atgagtgctg tctcgggcag caaagttaag tagaatccgc ggcgaccatt cgacaaaaga
     6661 ggtgaatcat gcaggattta tcattggaag cacgcctggc agagctggag agccgactgg
     6721 cttttcagga aatcaccatt gaagaactga acgtcacggt gaccgctcat gaaatggaga
     6781 tggcgaaact gcgcgatcat ctgcgtctgc tgaccgagaa gttaaaagcc agccagccgt
     6841 cgaacatcgc gtcgcaggct gaagaaacgc caccgccaca ttattgaggc gtaaaaaaag
     6901 cggggattcc ccgctttttt gtcacctttt cggtattagt ggcaaccaca accgccgttg
     6961 cctttaccgc cacagcagcc ttcgccaccg tgttcatgac cgtgatcgtg gccatgaccg
     7021 ccgcagcaac cgtcgtggtc gtgatcgtgg tgatgatcgt gcgcgccgtg aacgtggcca
     7081 tgagccagtt cttcttcggt cgcttcgcga atcgccacaa cttcaacgtt gaatttcagg
     7141 ttctgaccgg ccagcatgtg gttaccatca accactacgt gatcgtcttc aaccgcagtg
     7201 atttcaaccg gtaccggacc ctgctcagtt tcagccagga aacgcatacc tacctgcagt
     7261 tcatcaacgc ccataaatac gtctttagga acacgttgca ctaggttttc gtcgtactga
     7321 ccgtaagcgt cgttcgcgcc aacagcgaca tcaaatttgt cgccaacttc atgaccttcc
     7381 agcgccgttt ccaggccaga gatcagggaa ccgtgaccat gcaggtagtc cagcggcgca
     7441 ctcaccggag actcatcaac caacacaccg tcttctgtac gtacctgata ggccaggctg
     7501 accaccaggt cttttgctac tttcatgata tctcctgagc atgggaagaa tagtggcgca
     7561 gattgtagcg gaattctgca gccgtgtact cactagctta aaaaaactcg gcgcatatcg
     7621 ctagtccgga tgaaaaatcc cgatcacttg ctcatctttg tgaacgtgat cgcgggcttc
     7681 tttgtctgct tctcgcatct gatgtccgca cttaacacat tcaacaatat caatattatt
     7741 ttcgcgccac atcgccattg aatcctgcgc ctgacaggcc gggcattttg cgcccgctat
     7801 aaaacgtttt cggattgcca tccttaccct ctactcaaat tcatcccagc cgtccagctg
     7861 gcgtcgttct tgttgcattt cacgctggaa aatctcttcc agttcgcgtc gggcttccct
     7921 ggcgcgagaa atttgtacgg tatcagcatg catcgggatt aactcccgca gcattcgcat
     7981 atccaggcgg cgaaaatgca gttgcgcccg ctgtgcctga tgcggatgca tgccaagcgt
     8041 gaccagcgtc ttgcgcccca gctctaacgc actggagaat gtttcacggg aaaactgcgt
     8101 cacccctgcc tgtaataact catgcgcttc cacacgtccg cgcactcgcg caagaatatg
     8161 caaatgcgga aagtgctgct ggcatatttc caccagcttc atggtgtctt ccggctcgtt
     8221 acaggtaatg acgatagact cagcggcctc tgcacccgca gaacgtaaaa gatcgacctg
     8281 cgtggcgtcg ccgtaataaa ctttgtagcc atatttacgc atcaggttaa cggcgctgat
     8341 atcccgctcc agcacggtaa tgcgcatttt atttgccatc agcaaacgac caatcacctg
     8401 accaaaacgc ccgaagccca cgacaatgac ctggggttta tcatcgttga cccacggttt
     8461 ttcgtcttct tcttccggtc cgttaaactg gcgggatagc catttatcca ccagcttcat
     8521 cagcaacggc gtggtcatca tggaaagcgt caccgtcacc agcaacagcg ccatctggtc
     8581 gccctggaat aaacgttgtg aagaagcggt agaaaagagg acaaaggcaa actcaccgcc
     8641 ctgactcaac acgccagcaa actgcatccg ctctgagcta cgcacgccat acaatcgcgc
     8701 cagcagatac agcacgagaa ttttcaccgc caccagcaca accacgctta tcactaccca
     8761 caacagatgg gtataaagca ccccgaggtt gagcgacatg ccgacagaga taaagaacaa
     8821 accgagcagc aagcctttga agggatcgat agccgtttcc agttcatggc gatattcact
     8881 ttccgccagc agcacgcccg caataaatgt accgagcgcc atcgacagcc ccagcgcatc
     8941 cataaacaat gcggaaccca acaccagcag cagcgtcgcg gcggtgaaca cttcccgcac
     9001 gccagaagct gcaataaagc ggaataccgg acgcagtaaa tagcgcccac caatcagcat
     9061 gccgacaaac gccagcacct tcatgccgat cttcatccag tcgaaatgtt cgtctgccga
     9121 ccccgccaac aacggcacta acgccagtgc tgggatcact gccagatcct gaaacagcag
     9181 aaccgaaaac ccgagctggc cggattcgct gcgattcatc cctttctcac gcatcaattg
     9241 caacgccatt gcagttgaag acatcgcaag gccaatgcca ccgaccaccg ccgcctgcca
     9301 ggcgaaatcc gtcagcatca ataatcccgc cagcaacgcc gcgcttaaca gcacctgtgc
     9361 cacgcctacg ccaaaaatcg aacgccgcag ttgccaaagt ttggagggat tcaactcaag
     9421 gccgatgata aacatcagga ataccacgcc gagttccgaa aagtggagga tctcatcgac
     9481 gtcgctaata aaccccagcc cccacgggcc aattgcaatc cctgccagca aatatcccaa
     9541 cacagcgcca atccccagcc gcgatgccaa cggcaccgca gccaccgccg cgaagagaaa
     9601 cagcactcct gcgagtaaaa aatcggaacc ttccatcagc ggcctcctgg agacagcgga
     9661 tttgccaacc agtcaccgta ggctctggcg tggctcgcca gctcctttgc gctttgccgt
     9721 ctcgcccagt aaataatgat ggaacttaac caatgcatcc ggcacatgcc cgccgccagt
     9781 tcaaaggggc gcagcacatc gctcatcggg tagcgattca gcgcgtcata acggtaagca
     9841 ctttccggct cgccggtggt aatcacgtta cgccagtact ttcccgccag ttggtttcct
     9901 cccggcccgc tggcaaaacc acgacttaat acccggtcca gccactcttt cagtagcgcc
     9961 gggcagctat aggtataaag aggatgctga aagacaatca cctcgtgctc gcgcagtaat
    10021 gcctgctcac gggggatatc aataaaaaaa tcgggatagt gcgcgtaaag gtcgtgcacg
    10081 gtaacattgc tgagctgcgt ggccggttta agcagtaccc ggtttgccac cgagtcctga
    10141 gattccggat gggcatacag cagcaaaact ttcgctggct gagacatcat ccccctcccg
    10201 gtatggtttt tgtgtatagt cgctgttttg ggctaccatt gcgcccggtg cggcagctcg
    10261 cccatacatt acattatcat aatgataagt taacatagtc tgaacatacg gcgccttatg
    10321 attgttttct cctcgttaca aattcgtcgc ggcgtgcgcg tcctgctgga taatgccacc
    10381 gccaccatca accccgggca gaaagtcggc ctggtgggta aaaacggctg tggtaaatct
    10441 accctgctgg cattgctgaa aaatgaaatc agcgccgacg gcggcagcta cacctttccg
    10501 ggaagctggc aactggcgtg ggtgaatcag gaaacgccag cgttaccgca agcggcgctg
    10561 gaatatgtca ttgacggcga ccgtgaatat cgtcaactgg aagcgcagct gcacgacgcc
    10621 aacgaacgta acgacgggca cgccattgcg accattcata gcaagctgga tgctattgac
    10681 gcatggagta ttcgctcccg tgccgccagc ctgctgcacg gcctcggttt cagcaatgaa
    10741 caactggagc gcccggtaag tgatttctcc ggtggctggc gtatgcgtct taaccttgcc
    10801 caggcgctga tttgccgttc agacttgctg ctgctcgacg aaccgactaa ccacctcgat
    10861 ctcgatgccg ttatctggct ggaaaaatgg ttgaagagct atcagggcac gctgatcctg
    10921 atctctcacg accgcgactt cctcgatccg atcgttgata aaattattca gatcgaacaa
    10981 caaagcatgt tcgagtacac cggcaactac agttcgtttg aagtacagcg cgccacccgt
    11041 ctggcgcagc aacaagcgat gtacgaaagc cagcaggaac gcgtggcaca tctgcaaagt
    11101 tatatcgatc gtttccgtgc caaagccacc aaagcgaagc aggcccagag ccgcattaag
    11161 atgctggaac gtatggagct gattgccccc gcacacgtcg acaacccgtt ccgctttagc
    11221 ttccgcgcgc cggaaagcct gccaaatccg ttactgaaga tggaaaaggt cagcgcaggc
    11281 tatggcgatc gcattattct cgactcgatt aaattgaatc tggtccccgg ctcgcgcatt
    11341 ggtctgttag gccgcaacgg cgcaggtaaa tcgacattaa tcaaactgtt agccggtgaa
    11401 cttgcgccag tcagcggtga aattggtctg gcaaaaggga tcaagctcgg ctacttcgcc
    11461 cagcatcaac ttgaatacct gcgcgccgac gaatcaccta ttcaacatct ggcacgttta
    11521 gcgccgcagg agctggaaca aaaactgcgt gactacctcg gcggctttgg tttcctgggc
    11581 gataaagtaa ccgaagaaac gcgccgcttc tccggtgggg aaaaggcccg cctggtgctg
    11641 gcattaattg tctggcagcg tccgaatctg ctgctgctcg acgaaccgac taaccacctt
    11701 gacctcgaca tgcgtcaggc agtcaccgaa gcattaatcg agtttgaagg cgcgctggtt
    11761 gtcgtttcgc acgaccgtca tttgctgcgt tccaccactg acgatctcta cctggttcac
    11821 gatcgtaaag tcgaaccgtt cgacggcgat ctggaagatt atcaacagtg gttgagcgac
    11881 gtacaaaagc aggaaaacca gaccgacgaa gcgccaaaag agaacgcgaa cagcgcccag
    11941 gcacgtaaag atcagaagcg tcgggaagcg gagctgcgtg cgcaaaccca gccgctgcgt
    12001 aaagagattg cccgtctgga aaaagagatg gagaagctga acgcgcaact ggcgcaggcg
    12061 gaagagaaac tcggcgacag cgaactgtat gaccagagcc gtaaagcgga gttgaccgcc
    12121 tgcctgcaac agcaagccag cgccaaatcc ggcctggaag agtgcgaaat ggcgtggctg
    12181 gaagcccagg agcagcttga gcagatgctg ctggaaggcc aaagcaactg atggcgcaga
    12241 taacgacgac cgatgccaat gaatttagca gcagtgctga attcacccct atgcgcggct
    12301 ttagcaattg tcatctacaa actatgctgc cgcgtttgtt tcgtcgtcag gtgaaattca
    12361 ccccgtactg gcagcggctg gagttgcccg acggcgattt tgtcgatctc gcgtggagtg
    12421 aagaccccgc acaggcgaaa cataaaccgc gtttagtggt gtttcacggg ctggaaggca
    12481 gtctcaatag cccttacgcc cacggtctgg ttgaggcggc gcaaaagcgc ggctggctgg
    12541 gcgtggtgat gcattttcgc ggatgcagcg gtgaaccaaa ccgtatgcac cgcatttacc
    12601 attcgggcga aaccgaagac gccagttggt ttttacgctg gctgcaacgc gaatttggtc
    12661 atgcgccaac ggctgccgtc ggctattcgc tcggcggtaa tatgctggcc tgtttgctgg
    12721 caaaggaagg caatgatctc ccggttgatg cggcggtgat tgtctctgcg ccgtttatgc
    12781 tggaagcctg tagctatcat atggaaaagg gcttttcccg cgtttatcag cgttacttgc
    12841 tgaacctgtt aaaagccaat gccgcgcgca agctggcagc ctaccccgga acgctgccga
    12901 ttaatctcgc gcagttaaaa tcggtacgtc gcatccgtga atttgacgat ctaatcaccg
    12961 ccagaattca cggctacgcc gacgctatcg actattatcg tcagtgtagc gccatgccga
    13021 tgctgaaccg gatcgccaaa ccgacgctga ttattcacgc caaagacgat ccgtttatgg
    13081 atcatcaggt gatcccaaaa ccggaaagtc tcccctcgca ggtggagtat caactgactg
    13141 aacatggcgg tcatgttggc tttattggcg gtacattact tcatccgcaa atgtggctgg
    13201 agtcacgcat tcctgactgg ttaacaacgt atctggaggc gaaatcatgt tgattccgtg
    13261 gcaagacctc tcccccgaaa cgctggaaaa tttaattgaa agctttgtgt tacgtgaagg
    13321 caccgattat ggtgaacatg agcgtacact tgaacagaaa gtcgccgacg tcaaacgcca
    13381 gctacagtgc ggagaagcgg tgctggtatg gtcggaactg cacgaaacgg tcaatattat
    13441 gccgcgcagt cagtttcgcg aataacctgc aaccgtcggc ggacgagata atagtgatag
    13501 ctaagtcatg gagttactat gtctgccaaa catccggtca ttgcggtaac aggatccagc
    13561 ggcgcgggga ccaccaccac cagcctcgcg tttcgtaaaa tattcgcgca gttaaatctg
    13621 catgcagctg aggtggaagg cgacagtttt caccgttaca cccgcccgga aatggacatg
    13681 gcgatccgca aagcgcgcga cgccgggcgg catatcagct acttcggccc cgaagcgaac
    13741 gacttcggcc tgctggaaca aaccttcatt gaatacggtc agagcggcaa agggaaatct
    13801 cgcaaatatc tgcataccta cgacgaagcc gtaccctgga atcaggtacc gggaacattc
    13861 accccctggc aacctttacc agaacccact gatgtactgt tttatgaagg tttacacggc
    13921 ggcgtagtca cgccacagca taacgttgcg cagcatgtgg acttactggt cggcgtagtg
    13981 cctatcgtta accttgagtg gattcaaaaa ctgatccgcg acaccagcga gcgcgggcac
    14041 tcacgagaag cagtgatgga ctcggtagtg cgttcaatgg aagactatat caactacatc
    14101 acaccgcagt tttcccgcac ccatcttaac ttccagcgcg ttcccaccgt cgacacttca
    14161 aacccgttcg cggcaaaagg tatcccgtcg ctggatgaaa gctttgtggt gatccatttt
    14221 cgtaatctgg aagggatcga tttcccttgg ctgctggcga tgttgcaagg ctcattcatt
    14281 tcccacatca atacgttagt ggtacctggc ggcaaaatgg ggctggcaat ggaattaatt
    14341 atgctgctgc tggtgcaacg attgatggaa ggaaagaaaa tcgagtaact ttgctattat
    14401 gccggataaa atactatccg gcttcacaac gggatagtta agtcacgcgg caaccacttc
    14461 atacgagtga gtaatattca ccgctttttc cagcatcaac gccactgaac aatatttctc
    14521 ggcagagaga tcaaccgcac gcgcaaccgc tgcgtctttc aggtcgcgac cggtgacgat
    14581 aaaatgcaga ttaatgtgcg taaacaggcg tggtgcctct tcgcggcgtt cagaggtcaa
    14641 ttttacttca caatcgacca catcctgacg ccctttttgc aggatcgaaa ccacatcgat
    14701 ggcactgcaa ccacccgccg ccatcaacac catttccatc ggacttggtg ctttatcgcc
    14761 tgagttgccg tccattaaaa tctggtgacc agaggcggat tcgcccagaa aagttaaccc
    14821 ttcgacccac ttcactcgcg cttgcatttt tgctactcca ctgcgtcaat tttcctgaca
    14881 gagtacgcgt actaaccaaa tcgcgcaacg gaaggcgacc tgggtcatgc tgaagcgaga
    14941 caccaggaga cacaaagcga aagctatgct aaaacagtca ggatgctaca gtaatacatt
    15001 gatgtactgc atgtatgcaa aggacgtcac attaccgtgc agtacagttg atagcccctt
    15061 cccaggtagc gggaagcata tttcggcaat ccagagacag cggcgttatc tggctctgga
    15121 gaaagcttat aacagaggat aaccgcgcat ggtgcttggc aaaccgcaaa cagacccgac
    15181 tctcgaatgg ttcttgtctc attgccacat tcataagtac ccatccaaga gcacgcttat
    15241 tcaccagggt gaaaaagcgg aaacgctgta ctacatcgtt aaaggctctg tggcagtgct
    15301 gatcaaagac gaagagggta aagaaatgat cctctcctat ctgaatcagg gtgattttat
    15361 tggcgaactg ggcctgtttg aagagggcca ggaacgtagc gcatgggtac gtgcgaaaac
    15421 cgcctgtgaa gtggctgaaa tttcgtacaa aaaatttcgc caattgattc aggtaaaccc
    15481 ggacattctg atgcgtctgt ctgcacagat ggcgcgtcgt ctgcaagtca cttcagagaa
    15541 agtgggcaac ctggcgttcc tcgacgtgac gggccgcatt gcacagactc tgctgaacct
    15601 ggcaaaacaa ccagatgcta tgactcaccc ggacggtatg caaatcaaaa ttacccgtca
    15661 ggaaatcggt cagattgtcg gctgttctcg tgaaaccgtg ggacgcattc tgaagatgct
    15721 ggaagatcag aacctgatct ccgcacacgg taaaaccatc gtcgtttacg gcactcgtta
    15781 atcccgtcgg agtggcgcgt tacctggtag cgcgccattt tgtttccccc gatgtggcgc
    15841 agactgattt atcaccccga tatcaactat gcacttcgac aaacgctggt gctatgtttg
    15901 cccgtggccg ttgggttaat gcttggcgaa ttacgattcg gtctgctctt ctccctcgtt
    15961 cctgcctgtt gcaatattgc gggccttgat acgcctcata aacgtttttt caaacactta
    16021 atcattggtg cgtcgctgtt tgccacctgt agcttactga cacagctgct actggtaaaa
    16081 gatgttcccc tgcccttttt gctgaccgga ttaacgctgg tacttggcgt cactgctgag
    16141 ctggggccat tgcacgcaaa attgcttcct gcatcgctgc tcgccgccat ttttaccctc
    16201 agtctggcgg gatacatgcc ggtctgggaa ccattgctca tctatgcgtt gggcactctc
    16261 tggtacggat tgtttaactg gttttggttc tggatctggc gcgaacaacc gctgcgcgag
    16321 tcactaagtc tgctgtaccg tgaactggca gattattgtg aagccaaata cagcctgctt
    16381 acccagcaca ccgatcctga aaaagcgctg ccaccgctgc tggtgcgcca gcaaaaagcg
    16441 gtcgatttaa ttactcagtg ctatcagcaa atgcatatgc tttccgcgca aaacaatacc
    16501 gattacaagc ggatgctgcg tattttccag gaggcgctgg atttgcagga acatatttcg
    16561 gtcagtttgc atcagccgga agaggtgcaa aaactggtcg agcgtagcca tgcggaagaa
    16621 gttatccgct ggaatgcgca aaccgtcgcc gctcgcctgc gcgtgctggc tgatgacatt
    16681 ctttaccatc gcctgccaac gcgttttacg atggaaaagc aaattggcgc actggaaaaa
    16741 atcgcccgcc agcatccgga taatccggtt gggcaattct gctactggca tttcagccgc
    16801 atcgcccgcg tgctgcgcac ccaaaaaccg ctctatgccc gtgacttact ggccgataaa
    16861 cagcggcgaa tgccgttgct tccggcactg aaaagttatc tgtcactaaa gtctccggcg
    16921 ctacgcaatg ccggacgact cagtgtgatg ttaagcgttg ccagcctgat gggcaccgtg
    16981 ctgcatctgc cgaagtcgta ctggatcctg atgacggtat tgctggtgac acaaaatggc
    17041 tatggcgcaa cccgtctgag gattgtgaat cgctccgtgg gaaccgtggt cgggttaatc
    17101 attgcgggcg tagcgctgca ctttaaaatt cccgaaggtt acaccctgac gttgatgctg
    17161 attaccaccc tcgccagcta cctgatattg cgcaaaaact acggctgggc aacggtcggt
    17221 tttactatta ccgcagtgta taccctgcaa ctgttgtggc tgaacggtga gcagtacatc
    17281 cttccacgtc ttatcgatac cattattggt tgtttgattg ctttcggtgg taccgtctgg
    17341 ctgtggccgc agtggcagag cgggttattg cgtaaaaacg cccatgatgc tttagaagcc
    17401 tatcaggaag cgattcgctt gattctgagc gaggacccgc aaccaacgcc actggcctgg
    17461 cagagaatgc gggtaaatca ggcgcataac actctgtata actcattgaa tcaggcgatg
    17521 caggaaccgg cattcaacag ccattatctg gcagatatga aactgtgggt aacgcacagc
    17581 cagtttattg ttgagcatat taatgccatg accacgctgg cgcgggaaca ccgggcattg
    17641 ccacctgaac tggcacaaga gtatttacag tcttgtgaaa tcgccattca gcgttgtcag
    17701 cagcgactgg agtatgacga accgggtagt tctggcgatg ccaatatcat ggatgcgccg
    17761 gagatgcagc cgcacgaagg cgcggcaggt acgctggagc agcatttaca gcgggttatt
    17821 ggccatctga acaccatgca caccatttcg tcgatggcat ggcgtcagcg accgcatcac
    17881 gggatttggc tgagtcgcaa gttgcgggat tcgaaggcgt aatacaggct aaaccatcgg
    17941 ataaggcatt cacgccgcat ccgacatatt ttgcctgatg cgtcttattt acgccccaac
    18001 caccttcgcc accgcgtggg cgaaacgttg catcccctca tcgatatccg catcttccac
    18061 caccagcgac ggtgctaaac gcatcacatc cggtccggca ttcagcacca ttacgccaac
    18121 ctctgcaccc gcatacagga aatcacgcgc ccgacctttg tagtgtggtt tcagctccgc
    18181 gccaattaac agccccatac cgcgaatatc gctaaacaca tcgtactgct gatcgatctt
    18241 ctgcaaatgg tcaacaaaac gctggcgttt cgcctgaatg ccttccagca cttcaggggt
    18301 attgatgata tcaaacgccg cgcccgctac tgcacaggcc agaggattac cgccgtaggt
    18361 ggaaccgtga gaaccaggat gaaacgcaga agcaatttcc gccgtggtca gcatggcgct
    18421 aatcgggaag ccgccgccta acgctttcgc agaagtcaga atatccggcg tcacaccgta
    18481 gtacctgtaa gcaaacaaat cgccagtccg ccccatcccg cactgcactt catcaaacac
    18541 caataatacc tgatgttgat cgcacagctc gcgcaagccc tgcaaaaact ctggcgtcgc
    18601 tgccgtcaca ccgccctcgc cctggatcgg ctcaaccacc accgcacagg tgtgatcatc
    18661 catcaccgct ttcactgcat ggagatcgtt aaagggaacg tggatgatgt ctgccggttg
    18721 cggcccaaag ccgtcggaat attttggctg cccacccacc gaaacggtaa acagcgagcg
    18781 accatgaaaa gcgttatgga aggcaataat tttggttttg aacgggctat gacgcacaca
    18841 ggcgtaatgg cgtgccagtt taaaggcggt ttcgttagct tccgtgccgg agttcataaa
    18901 caccacgcgt tcggcaaacg tcgcctcaat cagtttgcgc ccaagacgca gcgccggttc
    18961 attggtgaaa acgttactga tatgccacag agtttcgccc tgggttttta acgcgttcac
    19021 cagcgcagga tggcaatggc ccaacgccgt aactgcaatg ccacccgcga aatcgacata
    19081 ctccttgcct tgctgatccc agattcggct gccctgacct tttaccggaa taaactctgc
    19141 cggagcataa atcggcagga tcacttcatc gaaagtcgcg cgtgtaattg ctgtttgttc
    19201 aattgccatc tcatgatcac cctgttacgc ataaataaat atgaaattat aatcacaaaa
    19261 tatgcataaa aaatcactga atggcaatcg gaaatcagag atgcaggaaa ttagccagca
    19321 gttgatgtcc ttgttcgcta agaatacttt ctggatggaa ctgcacacct tccagatccc
    19381 actggcgatg gcgaatcccc ataatctcgc gggtttcgct ccaggccgtc acgtcaaagc
    19441 acgctggtaa tgagtcaggt tccaccacca gcgaatggta gcgtgtcacg gtaagtggat
    19501 ttgccagccc ccgaaatacg ccctcaccgc tatgtgtaat cggcgaggtt ttgccgtgca
    19561 tgacctttgc ggcgcgcaca actttgccgc caaatgcctg cgccattgcc tgatgaccga
    19621 ggcagacgcc aagaatcggc aagcgcccgg catagtggcg aataacgtca agggagatcc
    19681 cggcttcatc tggcgtacag gggccaggtg agatgacaat tttttgtggt ttaagggcgt
    19741 cgatatccgc cagcgtcaac gcatcgttgc gcttaaccag cacatccgcc cccagttcac
    19801 aaaagtactg gtagaggttc caggtaaaag aatcgtagtt atctataagc aggatcatgg
    19861 cggctccggt acgaaagaac cgcactattt tactcagatt ccccggcttc gcttaccact
    19921 ttgctaaata tcatctgcag tgcggtgaga tcccccattg ccccactctg atttgcctga
    19981 ttccaagcct ctttttcgat accctgccag ctcagttgat atccggcatg aatcgccagt
    20041 tgctcgaaga agatccgctg tgccagacca cttcccaccc ggaagggatg cagcacgtcg
    20101 atttcacaat agtaatgcgc cagccgctcg acgaacttcg ctttctccag gccaaccaga
    20161 taaccttctt cctccagatc ctgcatcagg gcattgccct ctttttcgat ataagcaaag
    20221 tggcagaacg gcgtatcgcc ctgataaata tcaacttcac gcagttgccc tgcccagtcg
    20281 aaaatatcct ggtacagctg gcgatggata gtgcgcaaat gcggtaaacc gcgcaccagc
    20341 ggaccaagct caatggtcgc agcacgcagc gccgtcattt cgtaagcggc ctgttccagc
    20401 cgctgctgct ggcggatgtt cagccggtta cgcatgatat caaggccagg ataaagatac
    20461 ggatcgcgcc cttcgccgaa tttatcgctc atagtgcctc ctcagctctt caaggcgcgc
    20521 taaagcctct gcggcagtaa gagtgactaa aggcatctcg acgccttcaa gacggcgact
    20581 ggcctggaaa ttacgattac gctgaagctc ccagagacgg gacttttgct tatcggtgag
    20641 tttcttcact tgatgcctcc ctgaacgtgt ccatttgcca caagtataag cggcaaatcc
    20701 gggttacgcc gggagagcag aagcgcgggc aagattgccc gcgcgagaaa tcattacggc
    20761 aggactttag cggaaaggat aactaccggt tttgacggca cattctggta cggaccaaca
    20821 tcatgagtcg gcacctggga aatcttatcg gcaacgtcca tgcctttcac cactttacca
    20881 aataccgcgt aaccgaaatc acgctgacca tggtcaagga aggcgttatc ggcaacgttg
    20941 ataaagaact ggctggtggc gctgtctttg tcagcggtac gcgccatcgc gatggtgcca
    21001 cgcgtgttgc gcaggccgtt atcggcttca tttttgattg gcgggtttgg ttttttctgc
    21061 tgcatctgct cggtgaaacc gccgccctga atcataaagc caggaatgac gcggtgaaag
    21121 gtagtgttgt tataaaaacc gctgttcaca taatcgacaa agttttgcac agacactggc
    21181 gctttttgtt tatccagctc cagttcgatg ttaccagctg aggttgtcaa caatacgtgc
    21241 gggtcccctt tcgctgccat tgctgcggga gaaagagcag aaagagcgaa aacagcagcc
    21301 atcgccgcca gggtcgattt gaacatgaga tttccttaac aaacagagca gaaaaaaagc
    21361 aagtgaagag attctaaata gccttcaaga acaaggccat ccctttacct aattttacgt
    21421 atctgaaata tatgtaacct gacgatcact ttttattccg taatcaaaat cacctcttaa
    21481 aatgcaattt agcaaccgat tgcaataaaa catttaaaca gatcacaaaa tcacctaaaa
    21541 tcgcccgctc gcggcatatc ccgctatgcc attttatttt tgaacgcagg ccaatcatga
    21601 ctaacagcaa tcgcatcaag ctcacatgga ttagctttct ctcctacgca ctgactggtg
    21661 cgttggttat tgtcaccggg atggtgatgg gaaatatcgc cgattatttc aatctgcctg
    21721 tttccagtat gagtaacacc ttcaccttcc tcaacgccgg cattttaatc tctatcttcc
    21781 tcaacgcctg gctgatggaa atcgtcccgt tgaaaacgca gttacgtttt ggctttctcc
    21841 tgatggtgct ggcggttgcc ggtttgatgt tcagccacag cctggcgctg ttctcgacgg
    21901 cgatgttcat tctcggggtg gtcagcggca ttactatgtc gattggtaca ttcctgataa
    21961 cacaaatgta tgaagggcgt cagcgcggtt cccgcctgtt atttactgac tccttcttca
    22021 gtatggctgg gatgattttc ccaatgatcg ccgcgtttct actggcgcgc agcattgagt
    22081 ggtactgggt ttatgcctgc atcgggctgg tgtatgtcgc tatttttatt ctgaccttca
    22141 gctgtgagtt cccggcactg ggcaaacgtg caccaaaaac ggatgctccg gtagagaaag
    22201 aaaagtgggg gatcggcgta ctgtttctct ctgttgcggc gctgtgctac atcctcggtc
    22261 agttaggttt tatctcctgg gtgcctgagt atgccaaagg cctgggcatg agcctgaacg
    22321 acgcgggcac gctggtgagt aacttctgga tgtcatacat ggtcggcatg tgggcgttca
    22381 gctttattct tcgcttcttt gatttgcaac gcattctgac cgtactggct ggtctggctg
    22441 cgattctgat gtacgtcttt aacaccggaa caccagcaca tatggcgtgg tcaattctcg
    22501 ctctgggctt cttctccagc gcgatctata ccaccatcat caccctgggt tcacagcaga
    22561 ccaaagtacc gtcgccaaaa ctggttaact ttgtcctgac ctgcggaacc atcggtacta
    22621 tgttgacctt tgtggttacc ggcccgattg ttgaacatag cggtccgcag gcggcactgc
    22681 ttacagcaaa cggtctgtac gctgtcgtct ttgtgatgtg cttcctgtta ggtttcgtca
    22741 gccgtcaccg tcagcataac accctgacct ctcattaatt gctaatgccg gacggcacta
    22801 tcgtcgtccg gccttttcct ctcttccccc gctacgtgca tctatttcta taaacccgtt
    22861 cattttgtcc attttttgca caaacatgaa atgtcagaca attccgtgac ttaagaaaat
    22921 ttatacaaat cagcaatata ccccttaagg agtatataaa ggtgaatttg atttacatca
    22981 ataagcgggg ttgctgaatc gttaaggtag gcggtaatag aaaagaaatc gaggcaaaaa
    23041 tgagcaaagt cagactcgca attatcggta acggtatggt cggccatcgc tttatcgaag
    23101 atcttcttga taaatctgat gcggccaact ttgatattac cgttttctgt gaagaaccgc
    23161 gcatcgctta tgaccgcgta cacctctcgt cttacttctc tcaccacacc gccgaagagc
    23221 tgtcgctggt gcgcgaaggc ttctacgaga aacacggcat caaagttctg gtcggcgaac
    23281 gcgctatcac catcaaccgt caggagaagg tgattcactc cagcgccgga cgtaccgttt
    23341 tttatgacaa gctgatcatg gcaaccggtt cctacccgtg gatcccacca atcaaaggtt
    23401 ctgatactca ggactgcttt gtctatcgca ccattgaaga cctcaacgcc attgaatcct
    23461 gcgcccgtcg cagcaaacgc ggtgccgttg ttggtggcgg cctgttaggt ctggaagccg
    23521 ctggcgcgct gaaaaactta ggtattgaaa cccacgttat cgaatttgcc cctatgctga
    23581 tggcggaaca gcttgatcag atgggtggcg agcagctgcg tcgcaaaatc gaaagtatgg
    23641 gcgtgcgcgt tcacaccagc aaaaacaccc ttgagattgt gcaggaaggt gtcgaagcgc
    23701 gtaaaaccat gcgttttgcc gacggcagcg aactggaagt cgactttatc gtcttctcta
    23761 ccggtatccg tccgcgcgat aagctggcaa cccagtgtgg tctggacgtt gctccgcgtg
    23821 gggggattgt cattaatgat tcctgccaga cttccgatcc ggatatctac gccatcggtg
    23881 aatgcgcaag ctggaacaac cgtgtatttg gtctggtagc accaggctac aaaatggcgc
    23941 aggtcgccgt tgaccatatt ctgggtagcg aaaacgcctt tgaaggtgct gaccttagcg
    24001 ccaagctgaa actgctgggc gtagacgtag gtggtattgg tgatgcgcac ggtcgcacgc
    24061 ctggcgcacg tagctatgtt tacctcgacg aaagtaaaga gatctacaaa cgcctgattg
    24121 tcagcgaaga caacaaaacc ctgctcggtg cggtactggt gggcgatacc agcgactacg
    24181 gcaacctgct gcaactggtg ctgaacgcta tcgagctgcc ggaaaacccg gattctctga
    24241 ttctgccggc gcactcgggt agcggtaagc cgtctatcgg tgttgataaa ctgccggaca
    24301 gcgcgcaaat ctgctcctgc ttcgacgtca ctaaaggtga tctgattgct gccatcaaca
    24361 aaggctgcca cacagttgcg gcgctgaaag ctgaaaccaa agcgggtact ggctgcggtg
    24421 gctgtatccc gctggtcact caggtactga acgcggaact ggcgaaacag ggcatcgaag
    24481 ttaacaacaa cctgtgcgaa cactttgctt attcgcgtca ggaactgttc catttgatcc
    24541 gcgttgaagg cattaaaacc ttcgaagaac tgctggcgaa acacggcaaa ggctacggtt
    24601 gtgaagtttg taaaccaacc gtcggttcgc tgctggcctc ctgctggaac gaatacattc
    24661 tgaagccgga acatactccg ctgcaggatt ctaacgacaa cttcctcgct aacatccaga
    24721 aagacggcac ctactcggtg atcccgcgtt ctccgggcgg tgaaatcacc ccggaagggc
    24781 tgatggcagt gggtcgtatc gcgcgtaaat ttaatctcta caccaaaatc accggctccc
    24841 agcgcctggc gatgtttggc gcacagaaag gcgatctgcc ggaaatctgg cgtcagctga
    24901 ttgaagctgg cttcgaaacc ggtcatgcct atgcgaaagc actgcgtatg gcgaaaacct
    24961 gcgtgggtag cacctggtgc cgctacggtg ttggcgatag cgtcggcctc ggcgtggaac
    25021 tggaaaaccg ctacaaaggc atccgtacgc cgcacaaaat gaagttcggt gtctccggct
    25081 gtacccgtga atgttcagaa gctcagggta aagacgtggg tattatcgcc actgaaaaag
    25141 gctggaacct gtatgtttgc ggtaacggcg gcatgaaacc gcgtcatgcg gatctgctgg
    25201 cagcggatat cgatcgcgaa acgctgatca aatatctcga ccgcttcatg atgttctaca
    25261 tccgtaccgc cgacaaactg acgcgtaccg cgccgtggtt agaaaacctc gaaggcggca
    25321 tcgattacct gaaagcggtg atcattgacg acaaactggg gctgaacgct catctggaag
    25381 aagagatggc gcgcctgcgt gaagcggtag tgtgtgagtg gactgaaacg gtcaatacac
    25441 cgtccgcgca gactcgcttc aaacacttca tcaacagcga caagcgtgac ccgaacgtgc
    25501 agatggtgcc agagcgcgaa cagcaccgtc cggcaacgcc gtatgaacgt atcccggtaa
    25561 ctctggtgga ggacaacgca tgagccagtg gaaagacatc tgcaaaatcg atgacatcct
    25621 gcctgaaacc ggcgtctgcg cgctgttagg tgacgagcag gtagcgattt tccgcccgta
    25681 tcacagcgac caggtgtttg cgatcagcaa cattgatccg ttcttcgagt ccagcgtgct
    25741 atcacgcggg ctgattgcgg aacaccaggg cgagctgtgg gtcgccagcc cgctgaaaaa
    25801 acagcgtttt cgcttaagtg atggcttgtg catggaagac gaacagtttt ccgtcaaaca
    25861 ttacgaagca cgagtgaaag acggcgtggt gcagctgcgc ggttaatgtt ttaacgggag
    25921 gcgcaatgcc tccccttttt gcatggtcct gtaatgacct tcggtatatt gcaggacatt
    25981 ttttaaactt tttgttttat tttttgtttt tattttttaa aggataatca aatgtttaca
    26041 gacactatta ataagtgtgc ggctaatgct gcgcgcattg cacgcctgtc ggcaaataac
    26101 ccgctcggct tttgggtcag ctccgccatg gcgggcgcat atgtgggtct tgggatcatc
    26161 ctgattttca cgctcggtaa tttgctcgac ccgtccgtac gtccgctggt gatgggcgcg
    26221 acctttggta tcgccttaac gctggtgatt atcgccggtt ctgaactgtt caccggacac
    26281 accatgttcc tcacctttgg ggtaaaagcg ggcagcatca gccacgggca aatgtgggca
    26341 atcctgccgc aaacctggct gggtaacctg gtcggttccg tcttcgttgc catgctctat
    26401 agctggggcg gcggtagcct gctgccggta gataccagca tcgttcactc cgtcgcgctg
    26461 gctaaaacca ctgcaccggc aatggtactg ttcttcaaag gtgcattgtg taactggctg
    26521 gtttgcctgg caatctggat ggcgctgcgc actgaagggg cggcgaaatt tatcgctatc
    26581 tggtggtgtc tgctggcatt tatcgcgtcc ggctacgagc actctatcgc taacatgacg
    26641 ctgttcgcgc tctcctggtt cggcaaccac agcgaagcct acacgctggc gggtattggt
    26701 cataacctgc tgtgggtgac gctgggtaat actttatcag gtgccgtatt catgggattg
    26761 ggttattggt atgctacgcc gaaagcgaat cgtccggttg cggacaaatt taatcaaact
    26821 gaaacggctg ccggttaatt actaaggggt ttttacgtgg atcatttgcc tatattttgc
    26881 caattacgcg atcgcgactg tctgattgtc ggcggtggtg atgtcgcgga acgcaaagca
    26941 aggttgctgt tagacgcagg cgctcgctta acggtgaatg cattagcgtt tattccacag
    27001 ttcaccgcat gggcagatgc aggcatgtta accctcgtcg aagggccatt tgatgaaagc
    27061 cttctcgaca cctgctggct ggcgattgca gcgacggatg atgacacgct taaccagcgc
    27121 gtcagcgaag ccgctgaagc tcgtcgcatc ttctgtaacg tagtcgatgc gccgaaagcc
    27181 gccagcttta ttatgccgtc gattattgac cgctcaccgc taatggtagc ggtctcctct
    27241 ggcggcacct ctccggttct ggcgcgcctg ttgcgcgaaa aactcgaatc actgctgccg
    27301 ttgcatctgg gccaggtggc gaaatatgcc gggcaattac gcgggcgagt gaaacaacag
    27361 ttcgccacga tgagtgagcg tcgccgtttc tgggagaaat tgttcgttaa cgaccgcctg
    27421 gcgcagtcgc tggcaaacaa cgatcagaaa gccattactg aaacgaccga acagttaatc
    27481 aacgaaccgc tcgaccatcg cggtgaagtg gtgctggttg gtgcaggtcc gggcgatgcc
    27541 gggctgctga cgctgaaagg actgcaacaa attcagcagg cagatgtggt ggtctacgac
    27601 cgtctggttt ctgacgatat tatgaatctg gtacgccgcg atgctgatcg cgttttcgtc
    27661 ggcaaacgcg cgggatacca ctgtgtaccg caggaagaga ttaaccagat cctgctgcgg
    27721 gaagcgcaaa aaggcaaacg cgtggtgcgg ctgaaaggtg gcgatccgtt tatttttggc
    27781 cgtggtggcg aagagctgga aacactgtgc aacgcgggta ttccgttctc ggtggttccg
    27841 ggtattaccg cagcttctgg ttgctctgcc tattcgggta ttccactcac gcatcgcgat
    27901 tatgcccaga gcgtacgctt aattaccgga cacttaaaaa ccggtggcga gctggactgg
    27961 gaaaacctgg cggcagaaaa acagacgctg gtgttctata tggggttgaa tcaggccgcg
    28021 actattcagc aaaagctgat tgaacacggt atgcctggcg aaatgccggt ggcaattgtc
    28081 gaaaacggaa cggcagtcac gcagcgcgtg attgacggta cgctcacgca actcggtgaa
    28141 cttgctcagc aaatgaacag tccatcgcta attattattg gtcgggttgt tggcctgcgc
    28201 gataaactga actggttctc caatcattaa tttaacccgg gccagagaat tctggccttc
    28261 ttaatgactc tttttatata ttcagcaaat aaaacataac cccttaataa atattatctg
    28321 ctaatgggtt atattcaccg ttacagcgac ttacaaaatt aagtcattcg ctcactgacg
    28381 ccaattaaat attcctgtat cgttcttaac gagcagtctg cttatattca tataatcaat
    28441 gaatattaat taatatataa tacataggga atgtaaatga acaaatttat taaagttgca
    28501 ctggtaggtg cagtactggc tacgttaact gcatgtactg gtcatattga aaaccgtgat
    28561 aagaactgct cttacgacta cctgctgcac ccggcaattt ctatttctaa aatcattggc
    28621 ggttgcggtc ctactgcaca gtaagtctct gacagaaatc ggctaacacc ggtttaattg
    28681 cccgacgccc tctggatttt ccgagggcgt atttgtttct atgtccttct tgcccccgcc
    28741 cgtaaaaata ttttcacttt aaattcaatt ttataactac atccattagc cacagcgcga
    28801 tcctgtgcga aattttgtga tcttcctcca cattacacaa catcatatgt tgttatattc
    28861 atcatgcatt gtcatgttgc cttttaaatg actgcaaact ctcccctaca acgtattgga
    28921 caagaaaaag gtatcgctat gggaagccag gaactccaac gcaagctcgg attttgggcc
    28981 gttcttgcaa tcgccgtcgg gacaaccgtc ggctccggta tttttgtatc tgtgggtgaa
    29041 gtggcaaaag cagcgggcac gccgtggctt acggtgctcg cgtttgtcat tggcgggtta
    29101 attgtgatcc cgcaaatgtg cgtctatgcg gaactatcca ccgcttatcc ggaaaatggc
    29161 gcagattatg tttatctgaa aaatgccgga agccgaccgc tggctttcct ctccggctgg
    29221 gccagcttct gggccaacga tgcgccgtca ttgtcgatta tggcgctggc gattgtcagc
    29281 aatcttggct ttttaacgcc tatcgatccg ttgctcggta aatttatcgc cgccggatta
    29341 attatcgcct ttatgttgct acacctgcgc tccgttgaag gcggcgcagc gtttcagacg
    29401 ctaattacca tcgccaaaat tatcccgttc actatcgtca ttggccttgg gatcttctgg
    29461 tttaaagcgg agaattttgc cgcccttacc accactgcga ttggcgcaac gggcagcttt
    29521 atggcgctgc tggcggggat ctctgccacc agttggtcgt ataccggcat ggcctctatc
    29581 tgttatatga ccggcgaaat taaaaacccc ggaaaaacca tgccacgagc gctgattggt
    29641 tcctgtctgc tggttctggt gctctacacc ctgctggcgc tggtgatttc cggcctgatg
    29701 cccttcgaca aactcgccaa ttctgaaacg ccgatttccg acgccctgac ctagatcccc
    29761 gcactcggca gcaccgctgg gatctttgtt gccatcacgg cgatgatcgt cattcttggt
    29821 tcgctttcca gctgcgtgat gtaccagccg cggctggaat acgcgatggc gaaagacaac
    29881 ctgttcttta aatgcttcgg ccatgtgcat ccgaaataca acacgccgga tgtctccatc
    29941 atcctgcaag gggcgctggg gatcttcttc atcttcgttt ccgatctcac cagcctgctg
    30001 ggttatttca ccctggtgat gtgtttcaaa aataccctca ccttcggctc catcatctgg
    30061 tgtcgtaaac gcgacgatta caaaccgctg tggcgtactc cggctttcgg gctgatgacc
    30121 accctcgcca ttgcgtcaag cctcattctg gtcgcctcaa cctttgtctg ggcaccgatt
    30181 cccggcctta tctgcgccgt catcgttatt gctactggtc tgcctgctta cgccttctgg
    30241 gcgaagcgta gccgccagct caacgctttg tcgtaatgtt atctgaagaa aataaaatgt
    30301 tggatattga taaaagcacc gtggactttc tggtcaccga aaatatggtt caggaagtgg
    30361 aaaaagttct cagccatgac gttccgctgg tgcacgccat cgtggaagag atggtgaagc
    30421 gcgacattga tcgtatttat ttcgttgcct gcggctcgcc actcaacgcg gcgcaaacgg
    30481 cgaaacatct ggcggatcgc ttttccgatc ttcaggtcta cgccatttcc ggctgggagt
    30541 tctgcgataa caccccgtat cgcctcgacg atcgttgcgc ggtgattggc gtttctgact
    30601 acggtaaaac ggaagaggta atcaaagcgc tggagctggg ccgggcctgc ggcgcactca
    30661 ctgcggcgtt caccaaacgc gcggacagcc cgattacctc ggcggcggaa tttagcattg
    30721 attatcaggc cgactgtatc tgggaaattc acctgctgct ctgctacagc gtggtgctgg
    30781 agatgatcac cctcctcgcg ccgaacgcgg aaatcggcaa gatcaaaaac gatctcaagc
    30841 agttgccgaa tgcgctcggt catctggtac gcacctggga agaaaaaggc cgccagcttg
    30901 gtgaactggc cagccagtgg ccgatgattt ataccgttgc tgcgggtccg ctgcgtccgc
    30961 tgggttacaa agaaggcatt gtaacgctga tggaatttac ctggacgcac ggctgcgtga
    31021 ttgagagcgg agagttccgc catggcccgc tggagattgt cgaaccgggc gttccgttcc
    31081 tgttcctgct cggcaatgat gaaagtcgcc acaccaccga acgcgccatt aactttgtta
    31141 aacagcgtac tgacaacgtg atcgtcatcg attacgccga aatttcgcaa gggctgcacc
    31201 cgtggctggc accgttcctg atgttcgtgc caatggagtg gctctgctac tacctgtcta
    31261 tttacaaaga tcacaacccg gatgaacgcc gctattacgg tggtctggtg gaatattaat
    31321 ccctctcccg gcccggcaac gggccggatt tacgcaagga gttacccgat gaaaacaggt
    31381 atgtttacct gcggccacca gcggctgcct attgaacacg catttcgtga tgcaagcgag
    31441 ctgggttacg acggcatcga aatttggggc ggtcgcccgc acgcgttcgc gccggactta
    31501 aaagcgggcg gcatcaaaca aatcaaggcg ctggcgcaga cgtatcagat gccgattatc
    31561 ggttacacgc cagaaaccaa cggctatccg tataacatga tgctgggcga tgaacatatg
    31621 cgtcgtgaaa gcctggacat gatcaagctg gcgatatata tggcaaaaga gatgaacgcg
    31681 ggttatacgc tgatttctgc ggcccacgcg ggctatctca cgccgcctaa cgttatctgg
    31741 gggcggctgg cagagaactt aagcgaactg tgtgagtacg cggaaaacat cggcatggat
    31801 ctgatcctcg aaccgttaac gccgtatgaa tcgaacgtcg tgtgtaatgc taatgatgtg
    31861 cttcatgcgc tggcgctggt gccttcgccg cgcctgttca gcatggtcga catttgcgcg
    31921 ccgtatgtcc aggcggaacc ggtgatgagt tatttcgaca aactgggcga taaattacgt
    31981 catctgcata ttgtcgacag cgacggggcc agcgacacgc attacattcc tggagaaggc
    32041 aaaatgccgc tgcgggaact gatgcgcgat attattgatc ggggctatga gggttactgt
    32101 acggtggagc tggtgacgat gtatatgaac gagcccagac tctatgcccg ccaggcgctg
    32161 gaacgctttc gcgcgctgct gccggaggat gagagatgaa aaccctagcg acaatcggcg
    32221 ataactgcgt cgatatctac ccgcaactga ataaagcgtt ttctggcggt aatgcggtca
    32281 atgtggcggt gtactgcact cgctacggca tacagccggg atgcattacc tgggtgggtg
    32341 acgatgacta cggcacaaag ctgaagcagg atctcgcccg catgggcgtc gatatcagcc
    32401 atgtccatac gaaacacggc gttaccgcac aaactcaggt ggaactgcac gacaatgatc
    32461 gcgtttttgg cgactacacc gaaggcgtga tggccgactt tgccctgagt gaagaggatt
    32521 acgcctggct ggcgcagtat gacattgtgc acgcggcaat ctggggacat gcggaagacg
    32581 cattcccaca gctgcacgct gcgggcaaac ttaccgcttt cgacttctcc gacaagtggg
    32641 acagcccgct ctggcagaca ctggtgccgc atctcgattt tgcctttgcc tccgcaccgc
    32701 aagaagacga agcgctgcgt ctgaagatga aagcgattgt tgcccgtggc gcaggaacag
    32761 tgattgtcac gctgggtgaa aacggcagca ttgcctggga tggcgcgcag ttctggcgtc
    32821 aggctcctga accggtgacg gttatcgaca ccatgggtgc cggagattcg ttcattgccg
    32881 gattcctttg cggctggtct gcggggatga cattaccgca ggcgatggcg cagggaacgg
    32941 cgtgcgcggc gaaaaccatt cagtaccacg gtgcctggta ggtataacgt tggcgtgagc
    33001 atcttcacgc caacgtgctg ttacttgccg gaaaacgacc ctataatccg agtaattcat
    33061 tctttatttc agggtcgatt atgtcagcta cggaccgcta ctctcatcaa ctcctctacg
    33121 ctaccgtccg ccagcggctg ctggatgata tcgcgcaggg ggtttaccag gccgggcaac
    33181 agatccctac cgaaaacgag ctttgtacac aatataacgt cagccgcatt accattcgca
    33241 aagccatcag cgacttagtg gcagacggcg tactgatccg ctggcaggga aaaggcacct
    33301 ttgtacaaag ccagaaagtt gaaaacgccc tgcttactgt cagtggtttt accgattttg
    33361 gcgtctcaca aggcaaggcg acgaaagaga aagtgatcga acaggaacgg gtcagcgccg
    33421 cgccgttttg cgaaaagctg aacatccccg gaaacagcga agtgttccat ctctgccggg
    33481 tgatgtatct cgataaagag ccgctgttta ttgatagttc atggatcccg ctgtcgcgtt
    33541 atcctgactt tgatgagatt tacgtcgaag gaagctccac ctatcagtta tttcaggagc
    33601 gttttgacac gcgagtggtc agcgacaaaa agaccatcga tatctttgcc gccacccgcc
    33661 cgcaggcaaa atggctgaaa tgcgaactgg gcgaaccgtt gtttcgcatc agcaaaatcg
    33721 cctttgatca gaatgacaaa ccggtgcacg tctccgaact cttctgccgc gccaatcgca
    33781 tcaccttaac gattgataat aaaagacatt aaccgtaggc cggataagat gcgccagcat
    33841 cgcatccggc gatgctggcg cactgaattt tacatcccgc acgttcccct caccctaacc
    33901 ctctccccaa aggggcgagg ggagcgttca gtgcggaatt tagcttgatt gccgaacggt
    33961 gctatccagc attaaatact cgcaatactc tcacgcaaaa tccgcagcac cgtctcttca
    34021 ccgctgcgat tcgggttaat gcgaatcgca caatgttcga gttgtggatt cgcctggcga
    34081 aacgttcctg aaagtcgata gaagagcggc gggatttcat atttcgactc tgcaccgacc
    34141 gggtaaggca acgcgccacg cttttgcgct tcttccagca ccctggcggc aatcagctga
    34201 tgaaactcga caatcaacac cttcgactgc gcattggcaa tgacggcgct tttcacttcc
    34261 gccaccgcgc cgccgttaag caaagccagc aaccgttcgg atactcccgc ctgcaccgca
    34321 tgcatcactg gcgcgaacac cagaccacgc aagacttcca gcgcctgtga gccctggatc
    34381 tggctaccgc cggagtaaag cgtggcgcga atccgactaa taacatcggc atcgccaacc
    34441 actgcaccaa cgccctccgg cccaaacagc ttgaagcagg agaatgtcga gacattcgcg
    34501 ccgcattcac agccgattcg cgctaccttc atcaccgcat agttatcatc ggttaacgct
    34561 ggaacacctg ccgcgcgcaa cgttgccagc acatctgcca gcacatagct gtcctgcggc
    34621 tgctggcgcg tatgctgcac cagcgcggca tcaggttgtt gctcgtcgac gacctgcttc
    34681 agtgccaaca ggtcattgaa atcagcagta ataagcgtca gccccatctg ctcaataata
    34741 acccgtgtcg tcgggtaaac aggcgcgtca tgcaccagaa gacgctgccc cggtttgagc
    34801 aaagccgcca gcccggcgcg aatcgcgcca gtccccgcgc cctgcaccag cgccgcggcc
    34861 tgtgcgtgaa atgcatcagc cagcacctgc tccacccgtt gggtaatacg cggttgattc
    34921 agacctggag ttaaccctaa atcaccgcca gcaagaaact cgctgcctgg aaaatggcgg
    34981 caaatagtgt ccaccagcgc aaactgcttt tgctgcgctt cagcaagagt caggctttgc
    35041 aaaggaaacg tcttcatagg agccttcctt aagccggaac gaacaggcca agccagtaaa
    35101 gaacattcag cagaataccg gtgatcatta ctgccacgac cggtgccgcc attttctgta
    35161 ccggacggcc cagggattcg ttgaggaagt aaatagcaac cgcgatagag aatccggtat
    35221 aacccgccat cttaattgct gcgaaaatcg aaccgaccag cagcgccatt tccatcagca
    35281 tattcatggc gttacggatg ttatccgacg cattacgcac cgacgggtag cgtcccagcc
    35341 atttgccgat cgaacgaagc agcaagactt ccgccgaaat aaccactgcg cctaataccg
    35401 ctgcaaccat cggattcggc gagagatagc ccaccgcata aacaaaggta aagcccgcaa
    35461 ctgcatacac acccgttgct aaggcggtgg tggcaatcaa cggcacaaac cccagtccgc
    35521 gcataaattc tgccagagcc gcctgattaa tcagcgtttg cgattgttcc ggcgtcacgc
    35581 ctgcggaata cgctttctcc agtgtgaaga tcgaaacttc gctaccagca aaaatcttca
    35641 tactggcaac ggcggcaatc aatgctccca cgatggcgat ataaggtaag tttttgataa
    35701 tccgcgacgt gcgctcttca aacaccgaaa gcccgctggc atcgatgtca ttttcatcac
    35761 gatgacgcag gtcgtgagtt atcgcgatcc ccagcagcat caccatgcca ataaagattt
    35821 cgatggattc agggttaaga tgtgggaaat agcgcacgac taccacacgg gtcatcagaa
    35881 caaccacggc ggcgaccaga ctttgcttcc agccaaactg gtagaaaatc gccaccagcg
    35941 ggaacagtgc aaaagcagaa accaccggcg agcttaattc cccgaggcta cctaatacat
    36001 ccaccggcag cgcggtcagc agctggttaa ctggcaacag gcaagtaagg atcaacacgc
    36061 cccagatagc gccaagacca aacgccatca ggctgtttat cgccagtacg ccgaggatat
    36121 cggtaggaag aaagagtaac catgcgttga gcagccaggt tttcagggta aaagagatcc
    36181 ccaccgaggc cacaaaacca atgctcagac caaaagcgat actccccgcc tcgcgacggt
    36241 tcatatagcc ttcaatcagt tgcggcagga tcgggtggat gccgtcatga aaaacagccg
    36301 ccgagcgatg cgccagaagc gatgtcatac ccgtcaggca cgccaccacg ataatctgaa
    36361 tatacagatc catagtcgtg ccttttattt cagatggtta atcaacatcg gaatcgcgtg
    36421 ttcgacgtgc tcaacggaaa ggccaaacgc cactttccct tcagcgatca ttttggcgat
    36481 atgctcgtct ttcgctttat gccgcgcccg caccggtatt acacgcgcca atgtaataat
    36541 ctagttgccc ggatttcacc ttcattgccg cttccatgtc gttgtgaatg aaaacttcaa
    36601 aacagccagg agccgtcgct tcaatggttt ttttaatctg ctcacgctgt aagcctgcaa
    36661 cgccaatctt tttcatgagt ctgtccttat tggaaaaatt gagagggatt ttcacgtaac
    36721 atcacatcca catcggcctg actgaatcct gactggcaca attgcggaat aaaggtggtc
    36781 aataaatagt catagccata accaccattg gcttttaaat gggagcggcg cgtaatatcc
    36841 atcgacagca tgacgcggtt cagcaaccca cggtcacgta gcgcgtggag catcgcaatg
    36901 cgcttttcgt ccgggtagta actgttctta ccaatggtgt cgaactgcac gtacgcgccg
    36961 agatcgatca tcttcaaaat gttgtcgagg ttgtctttca gatcgcagtg accaacggtg
    37021 acgcgcgaaa gatcaactcc gtgggcttgc aacagagcca gttgttccag ccccatggtg
    37081 ctgaacgacg tatgcgtgga gatcgggcgt ccggtctggt tatgcgccag cgcagcggca
    37141 ataaatacct tctcttccag cggcgtaatc tttccttcgc tggagccgat ctccgcgatg
    37201 atcccggctt tcagctccgt gccatcgata ccctgttcaa tttcatcgac catctcctgc
    37261 gccagttcct gcacgctgcg ggtcaccaca tgttccggga aaaacgcatc ctggtaataa
    37321 ccagtacagg ccaccacgtt gatcccggtt tcgcgcatta catcgagcat aaattgcgca
    37381 ttgcgcccca tgtaacggtt ggtcatctca atcacattac gtacaccccg ggtcatcagg
    37441 tcgttcatct cctggcaaat gaacgcatac tgatcaaggc ggcagtccac gttgttttta
    37501 aagccggaga gatcaatatg cagatgctca tgggccaggg tgtaacccgt cggatcaaaa
    37561 ctcatagggt gtctcctgca aaccggaggg aggaaagaaa agagcgacca ttttgtggcg
    37621 gtggcgcacg gaaaaattca cacacggtag ctcccacatc agaaagcgtg gtgcgcacac
    37681 cgagctgcgt ggcgaccagc ccttgctgat aaaccagcac tggcaccact tcacgggtat
    37741 ggtggctgtg accaatggtc ggatcgttac cgtgatccgc catcacgacc aggcaatcat
    37801 ctggctgcat cgcctcaaca agccgggcaa ggttacggtc aacgacctgc aaacgttcgg
    37861 cataacgtgc gacgtcttct gcatgaccag cgaggtcggt ttcctggata ttgatgcaaa
    37921 taaacgccgt cggatgggtg ttaaattcgt cgagggtgat atccataatc cgctggctat
    37981 ccaccaggtt ttgccagctc acaccataag gattgctgac gatatctgcc accttaccca
    38041 ccagcacggt tggcacgccc gcttcataca gtttttgcgg cacctgcact ttttcatcaa
    38101 caccgtagcc catatgcacg acctggaagc cgttgtcata agcgccagaa cgcggcgcat
    38161 taataccaat aaagcgccct tctttgcttt ctgcggcatc gagaatacgc tgactgttgg
    38221 ttaacaggcc gccaaatgta atgacccgac cgacctgtac ctggtcacgc acgatacgac
    38281 caattttgat tgcgtcgtca aaagagatca cagagagatt ggcggtaatg ttatagactt
    38341 ggcctaaatc cgcctcgaga ttatcgccaa tcgcaaccgc ctgattgacc cacagaaatt
    38401 gcagatcatc gccacggcgc tccacctgcc agccagcgga aactaatgcc tgctcaacac
    38461 ggtcaatcac atcgcgaaaa ggcatccgca gcggcggtaa cgggcgcgtg cctaaaattt
    38521 cctgatgccc cataaaggta tcgccacctt catgttgcag ctctgccacg ccccaggttg
    38581 cggaatctga cggctgcata tcgcctggcg cataacccaa tgtgttgatt agccccagct
    38641 tctccaacgt tggtagctgc aaatgcggca actggctcag gatgtgacca caggtattcg
    38701 ccccggcatc ttgcggacgc accagcgtga catctttcat tgcccctacg ccaaagctat
    38761 caatcactaa caccacaaat cgcgccattt atgccttcag agggttgccg aggctgtcat
    38821 aacgaccaac tatttctggt tcgccgcgct gaataccgga caccagcacc acatcgctac
    38881 gggtgacaaa aatctgcgtg cgaaaacaga gcaccactgc actgctcacc ggatactcgc
    38941 ctgccagcgg cagggtatag tcgatactgc tgtcatccac ggttttgaga ttggtttcag
    39001 taatcttttg attttctggc gtaaacacca gcgcatgttg cgcatgacca cgacggtagt
    39061 aaccgccgcc gtagcagtag ctgtcgccac ggaaatgatg ggagatttca cttaaccaga
    39121 gcatcgcgat acgttcaggc tgatcgccct gctggtttgc cggaatagta ccggtcagcg
    39181 catgaccggg ttcggcatga gtcgcaccgt attccgccag caatggcagc gaagtgcagc
    39241 tggtcgctga aggtacgttc agttgctcaa tcgcaatacc agatttcgcc agttgatccc
    39301 gtgcttgtac cagcgtgtga agattcggtg ttggcaaaac tttcccggca gcctcatccc
    39361 aaagcaggca agggaaatgg gttagtccgg ctaaatgcag ccccggcagt ttcttaattt
    39421 cagcaatcac ctcgtgcagc gaatgctgta caaaaccgct ctcctgcccc ggataaagaa
    39481 aatcatcgtc gctataaact ttgagcagca caggctggac ccgtccggcc ttaaccgctg
    39541 ccgcagaaac ttcccgtgct ttgtcgagag taaacacggt gatgatgtcg gtgccctgtt
    39601 caacggcgtc agcaacctga tgacaaggga tttgtaccag atgcccctga tgcgccactg
    39661 gcaaaccagc gcggcgcatg actcgcgcct ctttgtaatc caccgccaca atgccgctgt
    39721 agcctaatgc cagcaatttt tccgccagcc acggattacg accaaattgc ttggtcatca
    39781 gatacagttc aatgccgtaa agccgcgccg tctcaatgag ccgcttaccg ttttccagta
    39841 tctgatccac gtcgatcacc cagctgtccg gggcgatttt gccctgctgc cacaaactta
    39901 gtgcggcgga aatcagcgcc gggttctggc gtttcaatgc ttctacaaac atcttgttgc
    39961 ctcgtttctg ttccttgaat taaatattca tttttttaaa tatttaggtt gtgcgacgtg
    40021 gatttgtcgg gagcttcaaa cctcttcgtt agcagccatc cataatccat aaagattggc
    40081 cagaaaatag ccttcttcac acgggttaac ttccagagcg aattccttca acaacacctg
    40141 atgcaattgc acaacggctt gccagtggct ggattgcgcc agttccgcca gcaactcgtt
    40201 atccagcggc tctatttctt caccacggcg actgcgcatc agtgcactcg ccatatgtgt
    40261 catcgccatc gttccttgct cactgcgcac cggcagatgg cactcttttt ccagtacgtt
    40321 gacgacctgc atcatgcctt tgcagacgtc cttatcaata acgcctgcct cgcaaagcag
    40381 gttgagtctg gtttccatat cagatgcctt tcgtttaata actgggttcc tgttccccac
    40441 tcaccacccg ttgttgatgg gcaagcaggg cgtgtttatc tacaacggca cgcagcaatt
    40501 gttgcattgg gtaatcatcg actcgcgtca ggaccacggc ttcggtggcc agtaaaaatc
    40561 gcggatcgtc tgttagcggc gtcgcctcta atcccagcat ggtcagttcg ttttccgcca
    40621 ccacgttcca gatcaccgca tcgacatcgc ctttaacaat gcgttgtaaa ctctcgtgat
    40681 aagagagatc gactcgttcc acatcactat cgccaaaaaa gacatcggtc atgattttct
    40741 gatccgccga acgactatcg agacctactc gcttaacatt tccagactca cctttgcggc
    40801 atatcagttg atgttcacca acatacgtat gcgggccaag ctcgagggcg atgcacaaat
    40861 tgttttggct gagataactt tccgccgcca ggcgggaaac tactgccatg tcatagacac
    40921 cgttaagtaa acactccacg cgaatatccg cgccgcgcat atgcgcatag taaaaaggaa
    40981 tgccatcaaa ctgggctttc aatccgctcg ctaaaccttc gtacaagcgg gtatagggca
    41041 aaggcatcgc acataccacg ttgttgatat ccacatgact cagcaatgct ttgttatcca
    41101 tctcgacaag ataactgcca ttgcgcccac ggcgttcaat ccgtatcgct ccgcttgatt
    41161 ccagcgtttt caacgcggcc tgcgtcaggc caaccgatga acggcattca gttgccagct
    41221 catctatggt tttcagtcga ttaccgcatt tttcacccaa caaataacgg gccagtgtcg
    41281 tgatgacgac gccttctttt ttgataaacg ttcgacgcat aatatatttt caataaaatg
    41341 aatatttata tcttcattaa aatgaagata agtggcgacg acgagatgtg gaaacggcga
    41401 ggcgcttcac attttttcgg gttgttgcga aaagaggata aaaaaaccgg gtttccccgg
    41461 tttcagagtg atgacgaagt gatttattcg acaatgcgag caaagggtaa ttcccactgg
    41521 cgcttgcctg ctcccctcac cctaaccctc tccccaaagg ggcgagggga ccgtccgagc
    41581 gttttgatat tacggcttcg ccacaaaacc aatcgcttcg tacaccgctt tcagcgtacg
    41641 ggaagcgtgt acactggctt tttctgcgcc gtctttcatc acctgttgca ggaaggcttc
    41701 atcgttgcgg aaacggtgat agcgttcctg caattcagtc agcataccgg aaacggcatc
    41761 agccacttca cctttcagat gaccatacat cttgccttcg aactgttttt ccagttctgg
    41821 gatgctctgg cccgttaccg cagaaaggat atccagcagg ttggaaacgc ccgctttgtt
    41881 ctgcacatcg tagcgaacta ccggcggctc gtcggagtca gtgaccgcgc gtttgatttt
    41941 cttcactacc gatttcggat cttccagcag gccgataacg ttattgcggt tatcgtcaga
    42001 cttggacatc ttcttggtcg gctccagcag cgacattacg cgcgcgccag atttcggaat
    42061 aaacggctcc ggcaccttaa agatctcgcc atacagcgcg ttgaaacgct gggcgatatc
    42121 gcgactcagt tccaggtgct gtttctggtc ttcacccacc ggtaccagat tagtttgata
    42181 cagcaggatg tccgcagcca tcagcaccgg atagtcaaac agaccagcgt tgatgttctc
    42241 ggcataacgc gcagatttat ctttaaactg cgtcatgcga ctcagttcgc cgaagtaggt
    42301 atagcagttc agtgcccagc ctaactgcgc atgttctggt acgtgggact gaacaaaaat
    42361 ggtgcttttc tcaggatcga taccacaagc cagatacaag gccagcgtat ccaacgtcgc
    42421 tttacgcagc ttctgtgcat cctggcgcac ggtgatcgcg tgttggtcaa cgatacagta
    42481 aatgcaatgg tagtcatcct gcatgtttac ccactgacgc agcgcaccca tgtagttacc
    42541 aatggtcaat tcacctgagg gctgtgcgcc actaaaaacg atgggcttag tcatttttcg
    42601 attcctgatt ttcgctatgc ggaagcccta atgcgggcag aaggtcattt atagactgat
    42661 aaattacatc aggctggctg agatcgatag cctcgccgta gttatatccg taggttaagc
    42721 caactgatgg gcaacctgcc gcttttgccg cctgaatatc attgcgtgag tcgccgacaa
    42781 acagcatctg ttgtggggca attcccatcc gctcagccac cagtaacagc gggtccggat
    42841 gcggtttttt gttttgcaca tcatcgccgc cgataaccac gctaaagtat ttggcaatat
    42901 ctaaggcttc gagcagcggc gcgacgaacg gcgtcggttt gttggtgacc aggcctagcg
    42961 gcaggccttt agcctgcaac gcgcccaacg tatcggcaac gtgcgggaac aaaaacgtcc
    43021 cctcttcggc aacctcgcca tagtagcgat cgaacagttt acgcagaata cgtacctgtt
    43081 cttctgccgg aatgtcgtca tcaacgggcg gtttccccat tgttttacgc agagtcgcac
    43141 gttcctgacg cgcccaggtc aatgcgcgct ccatcagaac atctgcgccg ttaccaatcc
    43201 aggtaataac gcgttcttca cctgcgacgg gcaactccag cgcatacagc gccatatcta
    43261 ccgcagcagc aagaccagga gcactgtcga ccagcgtacc atcaagatca aaagcgacgc
    43321 cgcgaatatc ttcaaactta ttcatgactt acctttgcca gttcactgcg catttcatca
    43381 atgacttttt tatagtctgg ctggtcgaag attgccgaac cggcgacgaa catatccgcg
    43441 cccgccgcag cgatttcgcc aatgttgttc accttcacgc caccgtccac ttccagacga
    43501 atgtcaaagc cagactcgtc gatacggcga cgtacttcgc gcagtttatc cagtgtttga
    43561 ggaatgaaag actgaccgcc gaaaccaggg ttgacggaca tcagcaggat cacatccagc
    43621 ttatccatca cgtaatccag atagctcaga ggtgtcgccg ggttaaatac cagacccgct
    43681 ttacagccat tttctttaat cagttgcagc gtgcggtcaa catgctcgga ggcttctgga
    43741 tgaaaggtaa tgatgctggc accagcggca gcgaaatcag gcacaatgcg atcgacgggt
    43801 ttcaccatca gatgtacgtc gataggggcg gtaatgccgt agttacgcaa ggatttcagc
    43861 accattggcc caatcgtcag attgggaaca tagtggttat ccatgacgtc aaaatgcacg
    43921 acatcagcgc cagctgccag ggcttttgcg gtatcttcac ccaggcgggc aaaatcagcc
    43981 gacagaattg agggggcaat caaatactgt ttcatccgct tctccttgag aattattttt
    44041 tcgcgggtga aacgactcct ggtttgtaca aagccagcag ttcgtccacc tttttacgtg
    44101 tgccgccgtt gctgcttata ctgcgtcgaa ctttgacgac atgcaatttt gcgcgctgat
    44161 accactcacg cgttaacatc gtatcgtgat tggagatcag cactggaata tggcgctcaa
    44221 ccagaccttc ggcgatctcc gccagatgcg cttgttgttc aagcgtaaaa ctgtttgtgt
    44281 gatacgccgt aaagttggcg gtcgcagaca gcggtgcata aggcggatcg caatagacga
    44341 cggatgcatc atctgcgcgc tccatgctat cggcgtaaga ctcacaatag aaaaaggcat
    44401 tctgcgcttt ttcagcgaag tgatacaact ctgcttccgg gaaatagggt tttttgtagc
    44461 ggccaaacgg cacgttaaac tcaccgcgca gattgtaacg acacaggccg ttgtaaccgt
    44521 agcggttcaa atataaaaac agtaccgccc gacggaacgg atcctggctt ttgttgaact
    44581 cttcgcggaa ctgatagtaa acctcggcgc aatttgtttc gggaacaaac agctcgcgtg
    44641 cggcctgtac gtactcatca gtacgcatct tcacaatgtt atagagactg atcaggtcgc
    44701 tattgatatc ggcaaggata taacgagaaa agtcggtgtt gagaaacacc gacccggcac
    44761 ctacaaaagg ctcaaccaga cattcgccct tgggcaaatg ccgtttaata tcatcaagca
    44821 ggggatactt gccccctgcc cacttcaaaa aagcgcgatt tttcttcatg ccgactaact
    44881 aattacacct tctccggctg tggagaaagc tccgacagca tccagcgctt caggcagctc
    44941 ccgcaacgat aaaccattgc gggagataac cttgattact tcagatcggc ctgtacctga
    45001 cgcagcggtt tcgcccacgg gtttttggcc tgaacatctg ctggcaatgt agataccgct
    45061 tttttcgcct cttctttcga agcgtacacg ccagaaacca ggacatacca cggctgacca
    45121 ttacgcgtcg tttcatagac aacgtagttt ttcagattct ctttcttcgc ccaaccgttc
    45181 aggttgtcgt agttagagga actgctcagc tgcagagtgt aatggctgga cggtgccgat
    45241 ttcaacgaac caacattacc tgcggtcttc cctccagcgg ctggtgttgc cgtggtttgc
    45301 gccggggatg ccgtctgtac tggagccgta gtcgccgtct cttttggtgc tggcgtagaa
    45361 gtcgcagccg gtgccttcgt gctcgccaca ggggcagctg gttcagtacg cttcggcgtc
    45421 tgtgctaccg gcttcggctc cgttttcacg gttgcttgcg gttttttcgg ttcaatcacc
    45481 gcctgctgac gaactgggcg cgtagtggac ggacgttcag cggtttgcgt tttcgccgtg
    45541 tcacgcgatg cattgccatt gcgaacaggc gcaaccgttg cgggttcagt cggcaacgtg
    45601 gaattgaccg ccacattgtt cagctgttgc tgattttgtg gctgggtcag cgcattgttc
    45661 aggtcaccct gcacttcaac acgttgttga ccatccgttg ccaccggggt ttgcccttga
    45721 gtcggcgtag aagagatcgg tggcagagaa acatcctgct gagtattttc cgcagacgtg
    45781 gttcccggcg ctggctgcac gccattcgcc tgatcggtcg cattgccagc aagatcaata
    45841 ctcttctcgc cagacgcggt ttgatcgctg gaagtggtcg agggggcttt tagcgcagaa
    45901 ccgataccga tgatcaacag cagtagaacc agaatgccga cgcccatcat catatactga
    45961 cgagaagcgg gtttactggc tgcttttttg cgcttacgcg gacgacgctc tacgcgctct
    46021 tcatccacgg tttcatcttc ggattcgtca atttcttctt cgatttccgg ttcctcattg
    46081 cgctcttttt gcgcacgagt cggacggcga tcgtcagtgt catcaagttc aatatcatca
    46141 aaattgatct gcggttcgcc acgttcagta cgctcagaac gttcagaaga ttgacgagaa
    46201 cgaccagtac gacgatcgct gggatcgggt ttcagctcgt cttctggttt gaattcatcc
    46261 atttaacacc ccactaaaag gttaatgctt accacgttgc aattaacctg aagctaatag
    46321 accgcttgat aagcggcctg acctttcttg ttgttacgct gattgacaat cggcaatggc
    46381 gttaagaaca agctcgtgcg aaacgccgct gcgaacttca ctcttaccaa ttgccaacgg
    46441 aagaattaag cgcatctctc ccgcaaggac tttcttgtca cgcagcatat gcggtaaata
    46501 cgcctgcgcg gacatttcgc gcggcccatt gaccggtaac ccagcccgcg tgagcagggt
    46561 tataatacgc tgcgtttcgg cagaactaaa ctgcccgaga cgttccgacg tccgcgccgc
    46621 catcaccata cccgcagcga ccgcttcacc atgtaaccaa ttgccatacc ccatttcagc
    46681 ttcaatggca tgaccaaagg tgtgtcccag attcagtaaa gcacgtaacc cggtttcgcg
    46741 ctcgtcggca gcgacaactt ctgccttcag ttcacaacaa cggcgaatac agtacgccat
    46801 tgccggaccg tccagacgca acaacgcatc cagattctct tccagccagt taaaaaacgc
    46861 accgtcaaga ataatgccgt atttgatgac ttctgccagc cccgacgcta actcacgcgg
    46921 gggaagcgtt ttcagacagt cgagatccac caccactgaa gcaggctggt agaacgcgcc
    46981 aatcatgttt ttaccgaggg gatggttgac cgcagttttg ccgccaacgg aggaatcgac
    47041 ctgcgacagt aacgtcgtcg ggacttgaat gaaacgaaca ccgcgctgat aactcgccgc
    47101 cgcgaagccg gtcagatcgc ccactacgcc gccgccaagc gccaccagcg tagtatcgcg
    47161 accatgcggc ttttgtaaca acgccgtaaa gacggtatcg agtacagcta ggcttttata
    47221 ctgctcgccg tcagggagga taacgctatc gacgttaaca cccgcctgtt caagtacgcc
    47281 gcggacctta tcgagataca gaggagccag ggtttcgctg gtgaccaaca tgacctgctc
    47341 gcccgatttc agcggtaaga atgaagctgg ttcattaaac aaaccagatg cgatggtaat
    47401 tgggtaacta cgttccccga gagtaacgac aatcctctcc ataacgcgac atccacctta
    47461 attactgtac ccgcagacga gtgtatataa agccagaatt agttgctttc cagcatgtga
    47521 ataatctggt ttgcaaccac tttagcgctt tgatcatcag tacgaatggt cacgtcggca
    47581 atctcttcat acagcggatt gcgttcattg gccaacgctt ccagaacttc acgcggcggt
    47641 gtttcaacgt gcagcaacgg gcgtttttta tcacgctgcg tgcgtgcaag ttgcttttcg
    47701 atggtcgttt caagataaac gacaacgcca cgagcggaaa gacggttacg cgtttcacgg
    47761 gatttcacag agccgccgcc agtagccagc acaataccct gtttctcggt caactcattg
    47821 atgacctttt cttcgcgatc gcggaagcct tcttcgcctt ctaaatcgaa aacccagccc
    47881 acatcagctc cggttcgttt ctcaatctct tgatcggaat cgtaaaattc catattgagt
    47941 ttttgagcta actggcgccc aatagtgctt tttccggcac ccataggccc aaccagaaag
    48001 atattgcgtt tctctgccat tttttcggta ctactaagac tattcgttaa tgataaaccc
    48061 gcttcgctca gagagcgccg caggacatga actgaaacct cataagatat tgcgagagtc
    48121 agactgaaaa ttatctcaat actccagcgg gtttggcaac tgaataaatc acccatccct
    48181 ctgcatatct ggtcgctgca agcgcactgc cttgctacca ccgctctggc gataaatcac
    48241 cgggtaagat tagcgtaaaa aagacagcaa aatgccgcct gaatgataaa tcatcatcat
    48301 ggaacgcagg cagcgaagag caccaactct caaatcggta ctccttgtat gctaaatacc
    48361 tgcgcgcgtc aaatagatga aacacgttca gcataaaaac attaccgctt ttacggctgt
    48421 ttactcactg gaaaccagtc gtggcgtgat aaacaccact aactcgcgtc gttcatcttc
    48481 ttttccgtca tgacgaaata attgcccgaa ccagggaatg tcgccaagca acggtacgct
    48541 atcctgaccc gatttatttt tacgggtaaa aatgccgccc agcgccaacg tttctccgct
    48601 ttttacctcg acctgcgttt cgatctcctg cttatcaatc gccagcactt cgccatcggc
    48661 ctgctgtagc acctgccccg gaacgttctg gctgatgtgt aatttcagcc ggatgcgacc
    48721 tttttgtaac accgtgggcg tgacctccat ccccaggacg gcctctttaa attccaccga
    48781 cgtcgcgcca ctttccccgc tggaaacctg atatggaatt tcgctcccct gtttaatgct
    48841 ggcaggctga agatgtgagg ccagcagacg cggactggcg ataatatcca gctgctgttt
    48901 ttgttcgagc gcggaaagct caagatccag caagcgtccg ttgatgcgcc caatgttaaa
    48961 accgacatgc gttgtcgccg tcgctacgga gaggtcgcta ccaagcgtgg tgacttgccc
    49021 aacgccacca gcgtgttgcg catcggccag cgtccatttc acgcctaact cacgcaaact
    49081 tttttcatta atggtgacaa tatgcgccga cagctcaacc tgcccgaccg gcagatccat
    49141 ttgcgctacc cactgttcaa gcgcgcttaa cgccgttttg ttatctcgta gcaaaaggcg
    49201 attggtgcgt ttatcgacgg tcatactccc tttggcactc agtagcttct cccccgcttt
    49261 cgccagttct cccgcgtcgg cgtattgcag ggttatactg cgattttcca gcggcagatt
    49321 tgcctgcgcc cgcgcctgct ccgcctcctg gcgggcgata ttgtcattct gccaggcaat
    49381 ggaatgcact gagagaatgt tgccttcctg ccgcgttatc agtccggcgc ttttcactac
    49441 agtttgtagt gcctgcttcc agggaacatc tgttagatgt aacgacaccg taccgctgac
    49501 gtctggcgac acgaccaggt tcaacttctc ctgttcagcc agcgcctgca acacctgagc
    49561 taccggaacg tcatccacca tcagcgtcac tttttgcggc tttgccgcct gtacgccgga
    49621 tatcagcatc aacagtagtg cggctatcca ttgcttcatt tgtatctcct tgccgttgcc
    49681 acaaccattg tggcggttcg cagtttgtcc cggtacccag cgttagtacg tctggcgtca
    49741 gctgtaaaat tgtccagccg ttttccagca catcgttttg ctgcacccgt cgccatttct
    49801 tttgcccgtc ttttattaca ccgatgatgc gctcgcctcg ccctaccatc ccctgatagc
    49861 gccactggct aagttcgcta atccggcata gatcttccgg cgatttaaaa gggtcacgca
    49921 taccggttaa aaggcacaat gcaatacctg ccaacaacca gcgtttaacc ctcatgcggc
    49981 atctccagtt gtagcgtgaa caaaagatca tcaccttcca cgcttaacga aaaacggctc
    50041 acgctgacgt tgcgctctgc cagccgtgta aatgccgacg gcactgcttc ccacagcgtt
    50101 ttcaacgcca actcgcctcc ctgcgcggat ggatgccagg aaaccagttg cgcgccggat
    50161 aactgaaaat ccagtggcga aaagggcagc gttttttcct cgctgaaggg agtggtgtct
    50221 accaggcgat acagtgcggc ccactgctgg tgatgacttg cccgtagtcg aattaatgcg
    50281 tcgcgctctt catggtgtgt cgatgataga aaaatgagcg taactaacat cagcaaccag
    50341 aatgcccagc aaaactggcg gaggcggggt gatgtggcga accaccagtc aaagaacatg
    50401 ttcatcgcta accttccttg ttaactgata ctcaaattgc cagcgtccct gcgcatcctg
    50461 ctgcgtggct ccccgctgat tgagatgaaa agacgcatcc tggcggagtg acgtttctag
    50521 tgcgtttaac gcggtaatgc ttgttgtcag ccccttgatc tccagcgttc cctgctgcca
    50581 gcttatcgtt gtcagccagg cgtgctcggg taaaagcgcc gccagtgctt ccagcgcaga
    50641 ttgccagtcg cgggtaaatt gtcgctggcg ctgccgttgc gaacgctgtt cgcgtaactg
    50701 ctgttgctcc agcaaacgtg gcttcgttat ctgtaagctg cgggcgagtt gttgttccgc
    50761 ctgaagcaaa actgcgtcta tgcgcgcttc ggcgctgcct gtcagacgca gtattagcgt
    50821 tatcccgacg gccagcagca gaggcgcaac gaacatcagc aaccagaaac gcagaaaagc
    50881 ggtccggcgt tgctgtcgcc agggcaaaaa attaattggc gggttcatca gtacgccttc
    50941 ccaagcgcca gccccagcgc gatggcaaag tctccaccag gcggcggtag cggcggctga
    51001 cgaacagaaa cggcctccca ggggtcaaat ccgccttcgc cacatatcgc gacgctgtcg
    51061 ggatcaacgg ataacgctgc cgccagctcc ttcgcgctag tcatccccac cgccagtttg
    51121 cgcccccagc tatagcgcgt cgcccacagc cactgttcgt tatcacgcca ggccagacat
    51181 tgctgatgag aaggtaaaaa aggcaggaat cgctgtaatg cactggcatc cggggtgatc
    51241 gcactaacat gaacacgcaa cctttctgcc agcgtaagca gcgttgccag ctctttgctt
    51301 tgcgcggcag tcacgttata agcggggctg agtgagtctt cgctgtaatc gaagcgcagg
    51361 gagtccggat ccatatccag ctcgcgggcc atcgtccctg acagccaggc cgtttgctcc
    51421 cgctcaccaa gggacatcga cgggcgcgga aatgaccgct gtaatgtgcg actggcggga
    51481 aacgccaaca taatgtgatg acgctgcggc agttcgcgac tccacggtaa caacgtttta
    51541 gccagctgct gcgcatcaac aatccgccca tctttgataa tgtcgtgcgc cagcggcaac
    51601 cgccaccagc gttgcaaaaa gcattctttt gcgccccgca cgatcgcaac cgctgccact
    51661 tcttgctgtt gtaaatgcaa accaatttgc cagatcttaa atgtcattgt gatgatctcc
    51721 ttatcacccg tcactctgac gggtatatca atgcgtctgg cttgccttta tactaccgcg
    51781 cgtttgttta taaactgccc aaatgaaact aaatgggaaa tttccagtga agttcgtaaa
    51841 gtattttttg atccttgcag tctgttgcat tctgctggga gcaggctcga tttatggcct
    51901 ataccgctac atcgagccac aactgccgga tgtggtgaca ttaaaagatg ttcgcctgca
    51961 aattccgatg cagatttaca gcgccgatgg cgagctgatt gctcaatacg gtgagaaacg
    52021 tcgtattccg gttacgttgg atcaaatccc accggagatg gtgaaagcct ttatcgcgac
    52081 ggaagacagc cgcttctacg agcatcacgg cgttgacccg gtggggatct tccgtgcggc
    52141 aagtgtggcg ctgttctccg gccacgcgtc acaaggggcg agtaccatta cccagcagct
    52201 ggcgagaaat ttcttcctca gtccagaacg cacgctgatg cgtaagatta aggaagtctt
    52261 cctcgcgatt cgcattgaac agttgctgac gaaagacgaa atcctcgagc tttatctgaa
    52321 caagatttac cttggttacc gcgcctatgg tgtcggtgct gcggcacaag tctatttcgg
    52381 taaaacggtc gaccaactga cgctgaacga aatggcggtg atagccgggt tgccgaaagc
    52441 gccttccacc tttaatccgc tctactcaat ggatcgtgcc gtcgcgcggc gtaacgtcgt
    52501 actgtcgcgg atgctggatg aagggtatat cacccagcaa cagttcgatc agacacgcac
    52561 tgaggcgatt aacgctaatt atcacgcgcc ggagattgct ttctctgcgc cgtacctgag
    52621 cgaaatggtg cgccaggaga tgtataaccg ttatggcgaa agtgcctatg aagacggtta
    52681 tcgcatttac accaccatca cccgcaaagt gcagcaggcc gcgcagcagg cggtacgtaa
    52741 taacgtgctg gactacgaca tgcgccacgg ctatcgcggc ccggcaaatg tgctgtggaa
    52801 agtgggcgag tcggcgtggg ataacaacaa gattaccgat acgctgaagg cgctgccgac
    52861 ctatggtccg ctgctgcctg ccgcagtcac cagcgccaat cctcagggag cgacggcgat
    52921 gctggcggac gggtcgaccg tcgcattgag tatggaaggc gttcgctggg cgcgtcctta
    52981 ccgttcggat actcagcagg gaccgacgcc gcgtaaagtg accgatgttc tgcaaacggg
    53041 tcagcaaatc tgggttcgtc aggttggcga tgcatggtgg ctggcacaag tgccggaagt
    53101 gaactcggcg ctggtgtcga tcaatccgca aaatggtgcc gttatggcgc tggtcggtgg
    53161 ctttgatttc aatcagagca agtttaaccg cgccacccag gcactgcgtc aggtgggttc
    53221 caacatcaaa ccgttcctct acaccgcggc gatggataaa ggtctgacgc tggcaagtat
    53281 gttgaacgat gtgccaattt ctcgctggga tgcaggtgcc ggttctgact ggcagccgaa
    53341 gaactcacca ccgcagtatg ctggtccaat tcgcttacgt caggggctgg gtcagtcgaa
    53401 aaacgtggtg atggtacgtg caatgcgggc gatgggcgtc gactacgctg cagaatatct
    53461 gcaacgcttc ggcttcccgg cgcaaaacat tgtccacacc gaatcgctgg cgctgggttc
    53521 agcgtccttc accccaatgc aggtggcgcg cggctacgcg gtcatggcga acggcggctt
    53581 cctagtggac ccgtggttta tcagcaaaat tgaaaacgat cagggcggcg tgattttcga
    53641 agcgaaaccg aaagtagcct gcccggaatg cgatattcca gtgatttacg gtgatacgca
    53701 gaaatcgaac gtgctggaaa ataacgatgt tgaagatgtc gctatctccc gcgagcagca
    53761 gaatgtttct gtaccaatgc cgcagctgga gcaggcaaat caggcgttag tggcgaagac
    53821 tggcgcgcag gagtacgcac cgcacgtcat caacactccg ctggcattcc tgattaagag
    53881 tgctttgaac accaatatct ttggtgaacc aggctggcag ggtaccggct ggcgtgcagg
    53941 tcgtgatttg cagcgtcgcg atatcggcgg gaaaaccggt accactaaca gttcgaaaga
    54001 tgcgtggttc tcgggttacg gtccgggcgt tgtgacctcg gtctggattg gctttgatga
    54061 tcaccgtcgt aatctcggtc atacaacggc ttccggagcg attaaagatc agatctcagg
    54121 ttacgaaggc ggtgccaaga gtgcccagcc tgcatgggac gcttatatga aagccgttct
    54181 tgaaggtgtg ccggagcagc cgctgacgcc gcctccgggt attgtgacgg tgaatatcga
    54241 tcgcagcacc gggcagttag ctaatggtgg caacagccgc gaagagtatt tcatcgaagg
    54301 tacgcagccg acacaacagg cggtgcacga ggtgggaacg accattatcg ataatggtga
    54361 ggcacaggaa ttgttctgat taaaaaggcg cttcggcgcc ttttcagttg gctgacaaag
    54421 tgcatttgtt tatgccggat gcgggtgaac gcgttatccg gctaacaaaa tcgtgaaaaa
    54481 tcaatatatt gcaggaaccc cataagcctg ataagcgttg tgcatcaggc aaacttcacg
    54541 catttacact cgcccctgcc ctttcaacca ttcgcgcacg aggaacagcg cgctgacatt
    54601 acgcgcttcg ttgaagtcag ggtcttccag caaatccatc atatgcgcca gcggccagcg
    54661 cacctgtggt agcggctctg gctcatcgcc ttccagcgat tccgggtaga gatcttgcgc
    54721 gaccacgata ttcattttac tggaaaagta agacggtgcc atgctgagct tcttcaaaaa
    54781 agtcagatcg ttcgcaccaa atccaacctc ttcttttagc tcgcggttag cggcttcgta
    54841 gacgctttca cccggatcaa ttaatccttt cgaaaaacct aattcgtagg attcagttcc
    54901 cactgcgtat tcgcggatca ggatcaggtg atcgtccaca atcggcacaa tcatcactgc
    54961 ttcccggttg gttggacgca ttcgttcata aacacgccgc acgccattgc tgaactccag
    55021 atccacactc tcgacggtaa acagtcggga acgggctaca gtttcaacat tcagaatggt
    55081 gggtttttgt aatgatttgt tcatcgtggg atctatgctg tgaaatcagc ggttattgtg
    55141 cgatatcgga cacgctttcg gcaatgtgaa ttgcatgtta tttacattta tgtaacttaa
    55201 taaataatcg tcctcaaatc aaattaaaag tcaataggtt gaaataactc caggaatttg
    55261 ctgatattcc gccttcagag ggtttgctat gatcagcggt tactgtgatg tgcttaatga
    55321 tgctcaagtt aaactccacg cttgccgata gccaaccgca gaatcatgta ttgtgtccgg
    55381 tgcgactgac cacgcctgac agactaagta agatggggaa agcatgagca ccattgtgat
    55441 ttttttagct gctttgctgg cctgctcact acttgcggga tggctgataa aagtgcgatc
    55501 cagacggcgt cagctgccct ggaccaacgc cttcgcggat gcgcaaacgc gtaaactcac
    55561 acctgaagaa cgtagcgccg ttgaaaatta tcttgagagc ctgacgcagg tattacaggt
    55621 gcctggccca acgggagcta gcgcggcacc gatctctctg gcgctgaatg ccgaaagcaa
    55681 caacgtcatg atgctgacac acgctatcac acgttacggc atctctaccg acgatccgaa
    55741 taaatggcgt tactacctcg attcggtaga agtccacctg ccccccttct gggaacagta
    55801 catcaacgat gagaataccg ttgaactgat tcataccgat tcgctgccgt tggttatttc
    55861 actcaacggt catacgctgc aggagtacat gcaggaaacg cgcggctatg ccttgcaacc
    55921 tgttccgtcc acgcaggcgt cgattcgcgg ggaagagagt gagcaaatcg agctactcaa
    55981 tattcgcaaa gaaacgcatg aagaatatgc gctgagtcgt ccgcgcgggc tgcgtgaagc
    56041 gttgctgatc gtcgcctcct tcctgatgtt ctttttctgc ctgattaccc cggatgtatt
    56101 tgttccgtgg ctggcaggcg gcgcattact gctgctgggc gcaggtctgt gggggctatt
    56161 cgcgcccccg gcaaaatcct ccctgcgaga aattcattgt ctgcgcggta caccccgtcg
    56221 ttggggattg tttggcgaaa acgatcagga acagatcaac aatatttcac tcggtattat
    56281 cgacctggtc tatcccgcac actggcagcc atacattgct caggatctcg gtcaaca
//
LOCUS       DADMGC010000011        53210 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.011, whole genome shotgun sequence.
ACCESSION   DADMGC010000011 DADMGC010000000
VERSION     DADMGC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.011"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(11..322)
                     /locus_tag="JNZ73_000671"
     CDS             complement(11..322)
                     /locus_tag="JNZ73_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HAY8731064.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="JNZ73_000672"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="JNZ73_000672"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="JNZ73_000673"
     CDS             701..1054
                     /locus_tag="JNZ73_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HAY8731065.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="JNZ73_000674"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="JNZ73_000674"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HAY8731066.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLHQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="JNZ73_000675"
     CDS             complement(2810..3280)
                     /locus_tag="JNZ73_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731067.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="JNZ73_000676"
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="JNZ73_000676"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HAY8731068.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="JNZ73_000677"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="JNZ73_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY8731069.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="JNZ73_000678"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="JNZ73_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HAY8731070.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="JNZ73_000679"
     CDS             complement(5283..8432)
                     /locus_tag="JNZ73_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAY8731071.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAVSLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="JNZ73_000680"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="JNZ73_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HAY8731072.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="JNZ73_000681"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="JNZ73_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HAY8731073.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="JNZ73_000682"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="JNZ73_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HAY8731074.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="JNZ73_000683"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="JNZ73_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HAY8731075.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="JNZ73_000684"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="JNZ73_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HAY8731076.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREAAAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="JNZ73_000685"
     CDS             14911..15288
                     /locus_tag="JNZ73_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HAY8731077.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="JNZ73_000686"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="JNZ73_000686"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAY8731078.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="JNZ73_000687"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="JNZ73_000687"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAY8731079.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     gene            18099..18428
                     /locus_tag="JNZ73_000688"
     CDS             18099..18428
                     /locus_tag="JNZ73_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAY8731080.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="JNZ73_000689"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="JNZ73_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAY8731081.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="JNZ73_000690"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="JNZ73_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAY8731082.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="JNZ73_000691"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="JNZ73_000691"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAY8731083.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="JNZ73_000692"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="JNZ73_000692"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HAY8731084.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23893)
                     /gene="aes"
                     /locus_tag="JNZ73_000693"
                     /pseudo
     CDS             complement(22933..23893)
                     /gene="aes"
                     /locus_tag="JNZ73_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24045..25349
                     /locus_tag="JNZ73_000694"
     CDS             24045..25349
                     /locus_tag="JNZ73_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HAY8731085.1"
                     /translation="MKFPGKRKSKNYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(25482..27158)
                     /locus_tag="JNZ73_000695"
     CDS             complement(25482..27158)
                     /locus_tag="JNZ73_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HAY8731086.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGKIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27396..28616)
                     /locus_tag="JNZ73_000696"
     CDS             complement(27396..28616)
                     /locus_tag="JNZ73_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8731087.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSQGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMISGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28834..30486
                     /locus_tag="JNZ73_000697"
     CDS             28834..30486
                     /locus_tag="JNZ73_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY8731088.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="JNZ73_000698"
     CDS             complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="JNZ73_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HAY8731089.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31206..32000)
                     /locus_tag="JNZ73_000699"
     CDS             complement(31206..32000)
                     /locus_tag="JNZ73_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731090.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32138..32479
                     /locus_tag="JNZ73_000700"
     CDS             32138..32479
                     /locus_tag="JNZ73_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY8731091.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32694..35198)
                     /gene="copA"
                     /locus_tag="JNZ73_000701"
     CDS             complement(32694..35198)
                     /gene="copA"
                     /locus_tag="JNZ73_000701"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HAY8731092.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35460..36392
                     /gene="glsA"
                     /locus_tag="JNZ73_000702"
     CDS             35460..36392
                     /gene="glsA"
                     /locus_tag="JNZ73_000702"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAY8731093.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36395..37687
                     /locus_tag="JNZ73_000703"
     CDS             36395..37687
                     /locus_tag="JNZ73_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8731094.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37812..38219
                     /locus_tag="JNZ73_000704"
     CDS             37812..38219
                     /locus_tag="JNZ73_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CueR"
                     /protein_id="HAY8731095.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38220..38678)
                     /locus_tag="JNZ73_000705"
     CDS             complement(38220..38678)
                     /locus_tag="JNZ73_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAY8731096.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTYVEVIAIEGITLHIRAVSS"
     gene            complement(38675..39592)
                     /locus_tag="JNZ73_000706"
     CDS             complement(38675..39592)
                     /locus_tag="JNZ73_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAY8731097.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39738..40415
                     /gene="fetA"
                     /locus_tag="JNZ73_000707"
     CDS             39738..40415
                     /gene="fetA"
                     /locus_tag="JNZ73_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HAY8731098.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40402..41181
                     /gene="fetB"
                     /locus_tag="JNZ73_000708"
     CDS             40402..41181
                     /gene="fetB"
                     /locus_tag="JNZ73_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HAY8731099.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="JNZ73_000709"
     CDS             complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="JNZ73_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HAY8731100.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="JNZ73_000710"
     CDS             complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="JNZ73_000710"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY8731101.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="JNZ73_000711"
                     /pseudo
     CDS             complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="JNZ73_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43551..44237
                     /locus_tag="JNZ73_000712"
     CDS             43551..44237
                     /locus_tag="JNZ73_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8731102.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44234..46648
                     /locus_tag="JNZ73_000713"
     CDS             44234..46648
                     /locus_tag="JNZ73_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8731103.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTDDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFVG"
     gene            complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="JNZ73_000714"
     CDS             complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="JNZ73_000714"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAY8731104.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48523..49449)
                     /gene="allS"
                     /locus_tag="JNZ73_000715"
     CDS             complement(48523..49449)
                     /gene="allS"
                     /locus_tag="JNZ73_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HAY8731105.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQHRPEISGFLEIFGNPRS"
     gene            49679..50161
                     /gene="allA"
                     /locus_tag="JNZ73_000716"
     CDS             49679..50161
                     /gene="allA"
                     /locus_tag="JNZ73_000716"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HAY8731106.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50239..51054
                     /gene="allR"
                     /locus_tag="JNZ73_000717"
     CDS             50239..51054
                     /gene="allR"
                     /locus_tag="JNZ73_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HAY8731107.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51144..52925
                     /gene="gcl"
                     /locus_tag="JNZ73_000718"
     CDS             51144..52925
                     /gene="gcl"
                     /locus_tag="JNZ73_000718"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HAY8731108.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALNSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52938..>53210
                     /locus_tag="JNZ73_000719"
     CDS             52938..>53210
                     /locus_tag="JNZ73_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HAY8731109.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12821 a        13706 c        14019 g        12664 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca acgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggtgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgac tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccggg aagggcagga tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctggc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aatcagcgag aatttgctgg cgtaatgtgt gctcttccgc tagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagct ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcat tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc cagccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaaccg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gtttttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttaaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagccgg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgtgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgcagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacccgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgctactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcatca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caatatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacta ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggcaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg cggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaagc
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactct gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacgg ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgttcgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggcgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggcgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca ggcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgac atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaacc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagccg gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctc atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc gccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactcctccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatca ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgccttcc atgcaaataa aatagcgtcg
    23641 ccgggctatc tggctgcgga caaaagagac gtgtttccac ctgcccatat tttgttggaa
    23701 ccatgtaagc tctggttgcc atttctggag cgcccgcatt ccagaatcgg cgctcaagcg
    23761 tgtaatactg tcgttgctca gcaatcgttc ccgttgcggg ccaggacggt aaatccggct
    23821 gaagagtatt cacaacggtc ttcatttcag cagaaataag gtccagaaca ggtagtttgt
    23881 tttccggctt catacaaaaa ctcctttcaa attacgtcat tgtaaggaac cactgccatg
    23941 aaaatgcgat cccgcctgct gatattgaaa ctggctgcgt ctcgcgcgct cccgtcagat
    24001 tgtgttaaca ttcgccgctc agttaaccac ccgtaaaaac aaccatgaaa tttcccggta
    24061 aacgtaaatc caaaaattac ttccctgtaa atgcacgcga tccgctgctt cagcaattcc
    24121 agccagaaaa cgaaaccagc gccgcctggg tcgtaggtat tgaccagacg ctggtcgata
    24181 ttgaagcgaa agtggatgat gaattcattg agcgttatgg attaagcgcc gggcattcac
    24241 tggtgattga ggatgacgta gccgaagtgc tttatcagga gctaaaacag aaaaacctga
    24301 ttacccatca gtttgcgggt ggcaccattg gtaacaccat gcacaactac tcggtgctcg
    24361 cggacgaccg ttcggtgctg ctgggcgtca tgtgcagcaa tattgaaatt ggcagttatg
    24421 cctatcgtta cctgtgtaac acttccagcc gtaccgatct taactatcta caaggcgtgg
    24481 atggtccgat tggtcgttgc tttacgctga ttggcgagtc cggggaacgt acctttgcta
    24541 tcagtccagg ccacatgaac cagctgcggg ctgaaagcat tccggaagat gtgattgccg
    24601 gagcctcggc actggttctc acctcatatc tggtgcgttg caagccgggt gaacccatgc
    24661 cggaagcaac catgaaagcc attgagtacg cgaagaaata taacgtaccg gtggtgctga
    24721 cgctgggcac caagtttgtc attgccgaga atccgcagtg gtggcagcaa ttcctcaaag
    24781 accacgtttc tatccttgcg atgaacgaag atgaagccga agcgttgacc ggagaaagcg
    24841 atccgttgtt ggcatctgac aaggcgctgg actgggtaga tctggtgctg tgcaccgccg
    24901 ggccaatcgg cttgtatatg gcgggcttta ccgaagacga agcgaaacgt aaaacccagc
    24961 atccgctgct gccgggcgct atagcggaat tcaaccagta tgagtttagc cgcgccatgc
    25021 gccacaagga ttgccagaat ccgctgcgtg tctattcgca cattgcgccg tacatgggcg
    25081 ggccggaaaa aatcatgaac actaatggag cgggggatgg cgcattggca gcgttgctgc
    25141 atgacattac cgccaacagc taccatcgta gcaacgtacc aaactccagc aaacataaat
    25201 tcacctggtt aacttattca tcgttagcgc aggtgtgtaa atatgctaac cgtgtgagct
    25261 atcaggtact gaaccagcat tcacctcgtt taacgcgcgg cttgccggag cgtgaagaca
    25321 gcctggaaga gtcttactgg gatcgttaag ttatcgtcgg ttcgtaggcc agataaggcg
    25381 ttcacgccgc atctggcatt tggctctcga tgcctgatgc gacgctggcg cgtcttatca
    25441 tgcctacata tttttcatat tttacatccg gcaaccaccg tttaccccgt caccacttca
    25501 cccgccggtg gcgtttccag cagttccagc atagtacggg caatttcacg ttcacccatc
    25561 actacctgat tcgcgccacg ttcggtgata tacgtcactt catcgtcata atgggcgcgg
    25621 gcgataatct caatatccgg gtttttcgcg cgggcagacg ccacaatctc acccgcttca
    25681 taaccgttgg ggatcgtcag gatcagccat tttgcacatt ccagatgtgc cagttgcata
    25741 atttcttcgt tcgctgcatt gcccaataca gcgcggaccc cacgctctcg cagctcatca
    25801 acacgggttc gtgacgtctc aatcaccacc agcggaatat cagaggcgag caatttctcc
    25861 cccagcaggc tgcctacacg accgtaaccc accaatagcg catggttgca aatatccact
    25921 gggatctgtt tctcttcttc gattgcctct tccagcgtct gctcttccag cgtttcggtc
    25981 ttcgccagat atttctccag tagtgcgaac agtaccgggt tgagcataat cgacaggatc
    26041 gcccctgcca gtaccaggtt ttgtccggcc tgcggcagta aattcaatgc cattcccagt
    26101 cccgccagga taaacgcgaa ctcaccaatc tgcgccaggc tggcggcgat ggttaatgcc
    26161 gtacgttggg agtgaccaaa cagacggaca aggaacaggg ccgcgaccga tttaccaaac
    26221 agaataattg ccagcgtcgc cagcactgcc agcggttgct gaatcagaat taacggatca
    26281 aacaacatcc cgactgagac aaaaaacagc accgcaaacg cgtcgcgcaa tggcaacgta
    26341 tcgtgggcgg cacggtgact caattcagac tcgttcagta ccatcccggc aaagaacgca
    26401 ccgagtgcaa aggagacatc aaacagctct accgcaccaa aggcaatccc taacgccagc
    26461 gccagcactg atagggtaaa cagttcgcga gaaccggttg ccgcgctgcg tgccataatc
    26521 cacggcacca gacggcgacc taccagcatc ataatggcga taaatgcgat caccttgccg
    26581 atggtgatcc ccatatcgac tgcaagagtg gcaaagccca catcgccctg ttccatcatt
    26641 cctgccactg cgggcagcaa caccagcgtc agaaccatca ccaggtcttc cacaatcaac
    26701 caaccgatgg cgattttccc acgctgactg tctattaatt gccgttcttc aagtgcgcgc
    26761 agtaacacca cggtactggc ggtggaaaga cataagccga acacgatacc ggtcattaac
    26821 gaccagccca gcacggcgga gagcgccata cccagcagcg tcgccacggc tatctgggcg
    26881 atcgcgccgg gaatggcgat ggactttacc gccatcaaat ccttgagcga aaaatgcaaa
    26941 ccgacgccga acatcagcaa aataacgccc agttcagcca gttccggcgc aagcttggta
    27001 tcggcaacaa agcccggagt gaatggtcct gccagcacac ccgctaacag atatcccacc
    27061 agaggagaaa tacgtagttt attggccagc atgccgagga taaaggcgag cacaaggccg
    27121 ccaacaatgg tggtgataag cggggtggcg tgatgcattc cgtctccttt tcctggtggt
    27181 tattgtccat ttttggccgg gaaaaccaaa attacaggta atagtttatg acaatttcat
    27241 tgatgatgtt tatgaataat tgttgaattt tgcagaaaaa tggaattagc tgcaaaaaaa
    27301 gcacggatcg gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg actgtaaaac
    27361 gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg gttatcaggc
    27421 aggaatatgg tcaacatccc caatagtggc aggaaagcac agattttata gactaactcg
    27481 atgctggtgt gatcggcgat aagccccaga actgccgctc ccagacctcc catgccgaaa
    27541 gcaaaaccga aaaagagtcc agaaatcata ccgatacgtc ccggaagtag ctcctgagcg
    27601 tagaccagaa tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc
    27661 cccgtccagt gcaggcgggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata
    27721 gagccccaaa tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta
    27781 cctgccgcaa cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat
    27841 ccgaattttt gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat
    27901 ttcgagaaaa tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt
    27961 ggattgatta tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa
    28021 cggctaattt gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg
    28081 ttgcctttgc cataaggcgc gataatcacc gccgccagca aggggccctg ggaactgcca
    28141 aagttgccgc cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata
    28201 cgggctacgc gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgcc
    28261 gccagcagaa ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc acttaaggta
    28321 aagcacatgc caattggcaa cgaccacggc atcggatatt tatcggtcca gtagccgacc
    28381 actggttgca gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata
    28441 aatgtcagag aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc
    28501 atgtcgttca gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc
    28561 ttggtcgttg acgcagccgc gcccgccaca ggctgggttt gttcactcat tgccatagga
    28621 aagtcacttt ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact
    28681 aacatacctg tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct
    28741 ggatgcagat ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt
    28801 ttaacacgtt gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg
    28861 ttagcgctgt taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat
    28921 aaaacctaca aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat
    28981 gaatatggcg aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag
    29041 gttgcggctg aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg
    29101 cccgagtctg acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat
    29161 gacgcgatgg cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag
    29221 gaaaagtggg ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag
    29281 cgcctgttta aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg
    29341 ctgacaaccg atgacacagc aaaaattggt aacccggaat acttcactga tatcgaattt
    29401 cgtaagcccg ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca
    29461 gacattatta tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac
    29521 gcaccgggcg atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc
    29581 ggtggtcact cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac
    29641 gtgccgggta cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat
    29701 gagtggggca aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg
    29761 gttaactacc agctgattcc ggtgaacctg aagaagaaag tgacctggga agacgggaaa
    29821 agcgagcgcg tgctttacac tcctgaaatc gctgaaaacc agcaaatgat ctcgctgtta
    29881 tcaccgttcc agaacaaagg caaagcgcag ctggaagtga aaataggcaa aaccaatggt
    29941 cgtctggaag gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt
    30001 ctggcagccc aaatggatcg cactggtgcc gactttgcgg tgatgagcgg aggcggaatt
    30061 cgtgattcta tcgaagcagg cgatatcagc tataaaaacg tgctgaaagt gcagccattc
    30121 ggcaatgtgg tggtgtatgc cgacatgacc ggtaaagagg tgattgatta cctgaccgcc
    30181 gtcgcgcaga tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg
    30241 gcgaaagacg gcaaactgaa cgaccttaaa atcaaaggcg aaccggtcga tccggcgaaa
    30301 acttaccgta tggcgacatt aaacttcaat gccaccggcg gtgatggata tccgcgcctt
    30361 gataacaaac cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat
    30421 atccagaaaa gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg
    30481 cagtaatccg aaagtgccgg atgtttgcat ccggcacaat gcttaatcgc ggcgggcgat
    30541 atcagcaaat ttggcatcga ggatctttgc cagatcgcct gccgccagtt cgatatccag
    30601 tccgcgcttg ccgccggaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat
    30661 cgtgggcaga cgttttttct gccccagtgg gctaatcccc ccaaccaggt atcccgtcga
    30721 acgctgcgcg accatcggat cggccatctc aactttcttg gcacccagcg cttttgctac
    30781 ttttttaaga tccagttgac ctgcgaccgg cgtaacggcc acggcaaggt gtttcatatc
    30841 accgttcact gccaccagca gcgttttgta gacctgatcc ggattcaaac ctaatttttt
    30901 gacgacttca tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa
    30961 cgaaatcttg tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta
    31021 aacaaaaaaa cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc
    31081 taagagtatt ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt
    31141 gacggcataa taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca
    31201 tttttttagc gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc
    31261 gcgaccacat atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc
    31321 tgatgcatca gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta
    31381 ttttgcggcg gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta
    31441 tgccagtggg tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac
    31501 atggcaatct ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc
    31561 gcctgcaata gctggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc
    31621 gtcgcctgaa gaaccatcgc gatttgccac agcggttggg tagaaaagag tgagggagaa
    31681 atgcccatct cctggctaat gtgctgcagg ttttggagtt gctcctcgct aatgcgctct
    31741 tccagcgcct cgcaggcagg caaattagca aaaggcgtat cgctggtgga aacatccgcc
    31801 tcgacgatca gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc
    31861 atatcgtggc tacccatatg aatactgcca atcagatgaa aatggcgatt gccggggagg
    31921 gtgatatcga tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt
    31981 acccggtaca acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga
    32041 gggatggcgc aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct
    32101 tgcatcgcta ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc
    32161 gactacaccc ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa
    32221 tgagttagca gagttgctgc atgttcatcg taatcgcgtc agtgcactga tcaataacaa
    32281 tcgtaaactc accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga
    32341 ttttaggcta aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac
    32401 ccaggaagaa ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg
    32461 tgcaaaaaag gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt
    32521 cacgccgcat ccggcatccg agcaccattg cctgatgcga cgcttacgcg tcttatcagg
    32581 cctacaaacc tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc
    32641 atgcgtcgat gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc
    32701 ttcggtttaa accgcagcaa ccggttggcg ttactcacta cggtaatcga cgagagcgcc
    32761 attgccgctc cggcaactac cgggttaagc agtgttccag taaacggcca caaaataccg
    32821 gcggcgaccg gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg
    32881 ttgcgcagcg ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc
    32941 atcagggtaa tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg
    33001 acatccgcct gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga
    33061 cgtccttcac tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca
    33121 atcacttcat caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg
    33181 gtcaacatca ccagacgata tcccgcttta tgcaggcgtt gcagcgccgc cacgctatca
    33241 ctacgcaacg gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc
    33301 acaggcgttg ctccttgcga tgcctgagct gaaatatcgg cttcaatcgc tttagtatcg
    33361 acttgctgat catttaacaa cgcctgatta cccagcaata acgcatgacc ttctgcttca
    33421 ccgctcacgc ccagcccgcg caatgtgcgg aaaccgttga cctgcggtag ctgcatatcg
    33481 cctgctttat caaggatcgc tcgtgccaat ggatggctgg aaccttgctc cagcgccgcc
    33541 gccagacgca gcgcctgcgc ttcgtcaaca tcagcaaatg ttttcactgc gacaacctgc
    33601 ggcttccctt cggtcagcgt cccggtttta tcgaacacga cggtatcgag tgtactggcg
    33661 cgttgcagcg cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg
    33721 gaaataatcg acatcggcgt cgccagtccc agcgcacacg ggcaggcgat aatcagcacc
    33781 gtagtggcaa tcaccagggt atagacaatc tgcggtgccg gaccaaagaa ataccagatt
    33841 gccgcactga caagcgcaat aaccactact accggcacaa atacggctga gattttatcc
    33901 gccagctgac cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatcctt
    33961 gatagcgtgg tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc
    34021 actgtcccgg catggacgct atcgccttcg cctttttgct gcgggattgg ttcgcccgtc
    34081 agcatcgctt catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga
    34141 tcgccggtcg tcaggcgcag caacatacct ggctgcactt ctgccagagg cacgcttttt
    34201 tcaccttcgt cagtaaccag gcgcgccgtc ggtggggtta aatcgagtaa tttctccagt
    34261 gccttagaag aacgctgacg tgcgcgtgct tccagcatat ggccgagatt gatcagaccg
    34321 ataatcatcg cactggcttc atagtaaaga tgtcgcgctt ccatcgggaa ccactgcggc
    34381 cacaggttga cgctcatcga atagagccac gccacgcccg ttcccagcgc caccagcgta
    34441 tccatcgtcg ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgccg
    34501 gcgaaaacca tcaccgccag ggttatcagg ccgataacca accacaggct gcggttgtcg
    34561 gcggtgacca tcatgttatc gccgatcatc ccccagacca tcaccgggat acccaccgcc
    34621 agtgcgacaa ttgcctgcca gcggaagcgc ttcatcgtag cgacggcggt ttcttgctgg
    34681 cgttcgcggc gtttcgcgtc atcttcaatc gcttccgcgc cgtagcccgc tttttccacc
    34741 gcctgcacta aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg
    34801 tttacccgtg cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca
    34861 cagctggcgc agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc
    34921 gctgccggaa gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc
    34981 agcggtttag cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg
    35041 atcagctgtt ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgct
    35101 tgttccacat ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag
    35161 gacaggccgt ccaggttcag gtcgatagtt tgtgacataa aacactcctt taagacagtt
    35221 ttgactggct gtgataaagg ttaaaccttc cagaaagggg aaggtcaaga aattaataaa
    35281 ccaggcgggt aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac
    35341 atttatttct ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg
    35401 aaaatcggca caggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga
    35461 tgttagatgc aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac
    35521 ttaacggcgg acaaaatgcc gattacattc cctttctggc gaatgtacca ggtcaactgg
    35581 cggcagtggc tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc
    35641 gctttgcact ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg
    35701 gcccgcaggc ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag
    35761 ttatcgcctt agagttgcat ggcggcaaac cgctttcgcc actggtaaat gctggcgcta
    35821 ttgccaccac cagcctgatt aacgctgaaa acgccgaaca acgctggcag cgaattttac
    35881 atatccaaca gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg
    35941 aacaaacaac caacttccat aaccgggcca ttgcctggct gctgtactcc gccggatatc
    36001 tctattgtga tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca
    36061 atactgttga actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga
    36121 cgcataaacg cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg
    36181 aagggctgta tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg
    36241 gtgtaggtgg cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac
    36301 caccgctgga cgaagaaggc aatagtgttc gcggtcaaaa aatggtggca tcggtcgcta
    36361 agcaactcgg ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg
    36421 taacaaaccc ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc
    36481 ggggatcttc gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctggggcgc
    36541 ctttgctttt ggcggtattg tggcgatgtt ttccggttat gcctatgcgc gtctgggggc
    36601 gagctatccc agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt
    36661 cttttcgcta gcgctctcgt tgctatacct gttgacgctg gcggtgagca tcgccatggt
    36721 tgcccgtgct tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca
    36781 ccttattttg ctctacgcgt tggggatcat tgcggtgatg acgcttttca actccttaag
    36841 caaccatgcg gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt
    36901 attgctgatt attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc
    36961 ccccagctcc ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt
    37021 tggcatgatg gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc
    37081 gtttctggtg gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct
    37141 tagcgatgta tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc
    37201 ttctccgctg ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac
    37261 ggcttcaggc attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag
    37321 cgaacgcgaa ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa
    37381 catcatcgtc gtggtgttga taatgctgat gacggcggcg ctaaatttag actcactcgc
    37441 cagcgttgcc agcgccacct ttttgatctg ctatctggcg gtgtttgtgg tggcgatccg
    37501 cctgcgtcat gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt
    37561 gttggtgatc gttggcttta tctacagcct gtggtcccag ggcagccgtg cgttgatatg
    37621 gattattggt gcaatcttac tcagccttat tgtggcaatg gtcatgaagc gtaataaaac
    37681 cgtataacac ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg
    37741 cactggcaaa aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg
    37801 ttgcgcgaac gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca
    37861 ttcgcttcta tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc
    37921 gcacctacac gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg
    37981 gctttaacct ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca
    38041 gcgccgacgt caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg
    38101 agctgcaatc catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca
    38161 gcgccgactg cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggtgat
    38221 taagacgaga cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatac
    38281 gtacctgcac cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata
    38341 tgaccgcgcc cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc
    38401 ccgcgctggt ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat
    38461 ttccaccaca gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc
    38521 caacccagcg gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac
    38581 aacaaataac catttccgcc cagcatctcg gctgccagca gcaacccgcc gagactgagc
    38641 cagaaaatat gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc
    38701 tgtctttcac cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg
    38761 gcatcatcac tactttgctg ttactggagg aaccgatctg ctgtaacgct tcggtgtatt
    38821 tttgcgctac gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca
    38881 ttttggtggc gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg
    38941 ccgactgacg ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga
    39001 ggatttccgc ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg
    39061 ctttcatctg cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt
    39121 caatacgggt gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc
    39181 gtgagttgat gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg
    39241 tacggatgtt agtcatggtc aggttgatga tcgccagctc cagattgctg acttcatagg
    39301 cagcgcgtgg cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt
    39361 tatctttcga gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct
    39421 tgcgaccaat gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg
    39481 tatagcgacc aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg
    39541 cgccgacaat gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa
    39601 cctcctgttg taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg
    39661 attactgaaa gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc
    39721 agaaaaaggg aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat
    39781 atctggcggg tgatacgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat
    39841 ttaagttaat taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt
    39901 cattgatcag cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa
    39961 aaccagaaat ctaccgccag caagtctctt actgcgccca gacgccgacg ctgtttggcg
    40021 atacggtata cgataatctg atctttccct ggcagatccg tgaccagcag cctgacccgg
    40081 ccatttttct cgattttctc gaacgcttcg ctttgccgga cagcattttg acgaagaata
    40141 tcgccgagct atctggtggt gaaaaacaac gcatctcatt gattcgtaac ctacaattta
    40201 tgccgaaggt tttattgctg gatgaaataa ccagtgcgct ggatgaaagt aataaacata
    40261 acgtcaatga gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga
    40321 cacacgataa agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg
    40381 gagaaatgca ggaagcacgc tatgaactcg cataatatta ctaacgaatc attagcactg
    40441 gcattaatgc tggtggtggt ggcaatctta attagccata aagaaaaact ggcgctggag
    40501 aaagatattc tctggagcgt cgggcgagcg ataattcagc tgattattgt cggctatgtg
    40561 ctgaagtata ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc
    40621 tgctttaatg cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc
    40681 tcatcgttta ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca
    40741 gggtcgattg aatttatccc gatgcaggtg atccctgtcg ccgggatgat tgccggtaac
    40801 gccatggtag cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag
    40861 caacagattc aggagaaact gagtcttggc gcgacgccga agcaggcttc agcgatattg
    40921 attcgcgaca gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc
    40981 ttagtgagtt taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag
    41041 gcgattaaat atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc
    41101 attattgcct gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg
    41161 actcaattga agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg
    41221 ttcatgtgcc gaacaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc
    41281 gacgccagtg catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc
    41341 tgaccgtctg cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct
    41401 tcattgcgcc caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct
    41461 ggattctccg ccacctgctg ttgcaactgt tgaatttccg gcttatcagc cgcctgcttc
    41521 agcagttcga tttgcgctac cagcccctgg tagcgggtgt cctgatcctg caacggaatg
    41581 gttttcagca ccgcttccgc atcttcagaa cggttcagcg caatcagcgt ttctgccagc
    41641 agcaggccga tctccccgtt ctgattcgac aactgccagg cgtctttcag caatggcagg
    41701 gcatcggtgt aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct
    41761 tcgcgcggca gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg
    41821 aagccatcta ccggttgccc gttctggaac agatacacgg tcggaatcgc gcgcagacca
    41881 aactgggcgg caatcatctg ctccgcgtcg cagtccagct tcgccagaat aaattgcccg
    41941 ttgtactgcg ccgcgaggct ttccaggatc ggggttaact gcaaacagtg ctggctacgt
    42001 tcagaccaaa aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg
    42061 ttagattcgt taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat
    42121 attttctttg acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc
    42181 atcacgcgcc caggcagcag gcgctcgagc accattactg cccaggtcac cagcgttacc
    42241 ggatagcgca tcttcggctt ctcgctaata aaagcatggc gcactttgtc gaccaccgct
    42301 tccggtccca acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc
    42361 gtctggttga cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact
    42421 ttaattccgc tgtggcgtag ctccatgcgc agcgcgtctg accacgcctc cagcgcatat
    42481 ttactggccg cgtaagcgcc acgacccggc gtggagatta atcccatcac cgatgatgtc
    42541 atcacaatac gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg
    42601 tgtgcgccga aaaagttggc ggaaaactgc tgttccatct gcgcacggct gatggtggaa
    42661 agggggccat acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc
    42721 agggcgatca cctcgtctgc tgcgcgatta acactttctg gtgaatccag atcgatcaac
    42781 acgccggtaa atcccatgtt gttcatacgc tcaacatcat tcggttttcg gcaacctgcc
    42841 agcacatgaa aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa
    42901 catccggtaa ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta
    42961 tgagtcatga tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg
    43021 ggcgtcgcgg ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc
    43081 ttccataaaa aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc
    43141 gctaaaggct tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa
    43201 tggttcagcg ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg
    43261 ctgtggctga aaaccacgca aaccatcatt gcgcccagtt caaccagcac ccaacgcggc
    43321 tgatgctgtt tgagcagagc cggaaggcgc gccagccctt gttgcgaggt gtcgccgctg
    43381 atgctggcat taactaccga cgttttactc tgccacttat cattcaacaa ggcaggccag
    43441 gccgcgctgg cagacattcg atatccggcg ctcaggctat cacccagaat caataacgtg
    43501 tccgctgcgg cggcacggaa ggttaacagg accaggaaca ggaagggcaa atgccagcgg
    43561 aaaacattgt tgaagttcat catcttaaga agtccgtcgg tcagggggag catgaactct
    43621 ccatcctcac cggagttgag ctggttgtca aacgtggcga gaccatcgca ctggtgggcg
    43681 agtcgggatc gggtaagtca accttgctgg cgatcctcgc cgggcttgat gacggcagca
    43741 gtggcgaagt gagtctggtg ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa
    43801 agttgcgcgc gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt cctaccctta
    43861 acgcgctgga aaacgtcgag cttccggctc tgctgcgcgg tgagagtagc gcggaaagtc
    43921 gtaacggggc gaaagcgttg ctcgaacagt tagggctggg taaacgtctg gatcatcttc
    43981 cggcacagct ttccggcggt gaacagcaac gagtggcgct ggcacgagcc tttaatggtc
    44041 gacctgatgt gctgtttgcc gacgaaccca ccggcaacct tgaccgccag acgggcgata
    44101 aaattgccga cctgctgttt tccctcaacc gtgaacatgg caccacgttg attatggtga
    44161 cccacgacct gcaactggcg gcacgctgcg accgctgctt acggctggtg aacgggcagt
    44221 tgcaggagga agcatgattg cacgttggtt ctggcgcgaa tggcgttcgc cgtcgctatt
    44281 aattgtctgg ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag
    44341 cgatcgcatg gagaagggct taagccagca aagccgtgag tttatggcgg gcgatcgggc
    44401 gttgcgcagt tcacgcgaag tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct
    44461 gaaagtcggc aagcagctga ctttcgccac aatgaccttt gcaggcgaca caccgcagct
    44521 ggcgaacgtc aaagcggtgg atgatatcta cccgatgtat ggcgatctgc aaactaatcc
    44581 ccctggcctg aaaccgcagg cgggcagcgt attgctggcc ccacgcctga tggcactgct
    44641 taacctgaaa acggacgaca ccattgacgt gggcgatgcc accttgcgga ttgccggaga
    44701 agtgattcag gaaccggatt ccggttttaa ccccttccag atggctccgc gtctgatgat
    44761 gaatctggcg gatgtcgata aaaccggagc cgtgcagccg gggagtcggg tcacctggcg
    44821 ttataaattc ggcggcaacg agaaccagct cgacggctat gagaaatggt tgttacctca
    44881 gcttaaaccc gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc
    44941 gatggaacgc tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc tgctggcagt
    45001 ggcagcggtg gcggtagcga tgaatcatta ctgtcgcagt cgctacgatc tggtggcgat
    45061 cctcaaaacg ctgggggcag ggcgagcgca actgcgtaag ctaatcgtcg gtcagtggtt
    45121 gatggtgctg acgctttcag ccgttaccgg tggggccata ggcctgttgt tcgaaaacgt
    45181 gttgatggtg ctgctcaagc ccgttctgcc tgctgcacta ccgccagcca gcctctggcc
    45241 gtggctgtgg gcgcttggca ccatgacggt catctcgctg ctggtggggc tacgaccata
    45301 ccgcttgttg ctggcaacgc agcctttacg cgtattacgt aatgatgtgg tcgcgaacgt
    45361 ctggccgctg aagttttatc tgccgattgt cagtgtggtg gttgtgctgc tgctcgccgg
    45421 attaatgggt ggcagcatgc tgctttgggc ggtgctggcg ggcgcggtag tactggcttt
    45481 gctgtgcggt gtgctgggct ggatgctgct gaatgtactt cgccgcatga cgctgaaatc
    45541 gctgcctctg cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca
    45601 gctttcggca ttttcgctct cctttatgct gctggcactg ctgctggtgc tgcgtggcga
    45661 tctgctcgac cgctggcagc agcagctacc gccagaaagc ccgaactact ttttaattaa
    45721 catcgccaca gaacaggtag caccgctaaa agcgttcctc gcggaacatc atatagtccc
    45781 ggaatcattt tatccggtag tgcgggcgcg gctgacggcg attaacgata agccgacaga
    45841 aggtaatgaa gatgaggcgc ttaatcgcga actcaatctt acctggcaaa atacgcggcc
    45901 cgatcataat ccgattgtcg ccggtaactg gccgccaaaa gccgatgaag tgtcgatgga
    45961 agaggggctg gcgaaacgct taaacgttgc cctcggtgat accgtgactt ttatgggcga
    46021 tacccaggag ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg aaagtctgcg
    46081 gcctaatttc tattttattt tccctgaagg ggcattagac gggcaaccgc agagctggct
    46141 taccagtttc cgctgggaga atggcaacgg catgttgacg caactcaacc gccagttccc
    46201 gaccattagc ctgttagaca ttggcgcgat tttaaaacag gtcggtcagg tgctggagca
    46261 ggtaagtcgg gcgctggagg tgatggtggt gctggtcacc gcctgcggta tgttgctgtt
    46321 gctggcacag gtgcaggtgg gaatgcgcca gcgtcatcag gagctggtgg tatggcgcac
    46381 actcggtgcg gggaaaaaac tgctgcgcac gacattgtgg tgtgagttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tgtaactatg attttcttca tgtgctagat gaggactgcc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aacaaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc
    47341 cgtttcagaa taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac
    47401 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat
    47461 attctttaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    47521 gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc
    47581 gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt
    47641 cgtcgccata agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcattcaggc
    47701 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct
    47761 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    47821 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    47881 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    47941 ccagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    48001 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg
    48061 cggtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    48121 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    48181 gcggattttg caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg
    48241 ccaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    48301 caacggcggc gcgttcatcg ttattcatta acggcagatt gatagcggcg ggcattgcgc
    48361 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    48421 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    48481 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtgggttgcc
    48541 gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc
    48601 ttctaccgct ttgccgctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    48661 catcgttggg attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga
    48721 ttttggcaaa aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc
    48781 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcg
    48841 ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc
    48901 ttcaacgttc gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa
    48961 gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga
    49021 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    49081 ctgggtaaag gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    49141 atagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    49201 cagctcgctg ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    49261 ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt
    49321 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    49381 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    49441 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    49501 gatttatcag tatttttaca tgataatcct gttcaatttg tggaccaaat ctagttttgg
    49561 aaaaacattc caacttttgt attgatgttg ttctcttaag gttttagatt gcctgttatt
    49621 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    49681 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    49741 aacgcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    49801 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    49861 ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ctcaggcctt
    49921 tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    49981 cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa
    50041 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    50101 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    50161 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    50221 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gcaggccagg acaggcggag
    50281 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    50341 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    50401 tccacgacct ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt
    50461 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    50521 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    50581 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    50641 tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    50701 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    50761 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    50821 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    50881 ctgaattgca tagcttcagc aatttacgat gatgtcggta gcgttgttgc cgctatctcc
    50941 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    51001 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    51061 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt taaaagttgg aaaatttttc
    51121 caattaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    51181 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt
    51241 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    51301 gtgtttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    51361 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctcaattcc gcttctgctg
    51421 attccattcc tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag
    51481 attttcaggc cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    51541 ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg
    51601 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    51661 ttgatcctga catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    51721 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg
    51781 gggtaatcaa tgctgatgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    51841 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    51901 ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    51961 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    52021 ccgaagggcg caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt
    52081 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    52141 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    52201 agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    52261 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta
    52321 ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    52381 tcaactgtgg tcaggctggt ccgttaggct ggacgattcc ggcggcactg ggggtttgtg
    52441 ccgctgatcc gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga
    52501 ttgaagagtt agccgttggc gcgcagttca acattccgta catccatgtg ctggtgaata
    52561 acgcctatct cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc
    52621 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    52681 aagtagcgga aggtttaggt tgtaaagcta ttcgggtctt caaaccggaa gatattgcgc
    52741 cagcctttga acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtagtggaag
    52801 ttattctcga gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat
    52861 ttgaagatat cgccgataac gcagcggatg caccgactga aacctgcttc atgcactatg
    52921 aataagggag ataaatgatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat
    52981 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    53041 tgtttcctta tgactacgac attgaagaat taaaacaggt actggcgagt aataaactcg
    53101 aacatacgct gcacaattta ccggcaggtg actgggcggc gggcgagcgc ggtattgcct
    53161 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc agcagcgatt
//
LOCUS       DADMGC010000012        52864 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.012, whole genome shotgun sequence.
ACCESSION   DADMGC010000012 DADMGC010000000
VERSION     DADMGC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.012"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..354
                     /locus_tag="JNZ73_000720"
     CDS             <1..354
                     /locus_tag="JNZ73_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731110.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="JNZ73_000721"
     CDS             complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="JNZ73_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HAY8731111.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="JNZ73_000722"
     CDS             complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="JNZ73_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HAY8731112.1"
                     /translation="MKHLHQFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLPFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFSNAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3052..3261
                     /gene="mokC"
                     /locus_tag="JNZ73_000723"
     CDS             3052..3261
                     /gene="mokC"
                     /locus_tag="JNZ73_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HAY8731113.1"
                     /translation="MLNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVT
                     RKDLCEVHIRTGQTEVAVFTAYESE"
     gene            complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="JNZ73_000724"
     CDS             complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="JNZ73_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAY8731114.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="JNZ73_000725"
     CDS             complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="JNZ73_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAY8731115.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSSRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6877..7281
                     /locus_tag="JNZ73_000726"
     CDS             6877..7281
                     /locus_tag="JNZ73_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HAY8731116.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7307..8020
                     /gene="msyB"
                     /locus_tag="JNZ73_000727"
     CDS             7307..8020
                     /gene="msyB"
                     /locus_tag="JNZ73_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAY8731117.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8169..8735
                     /gene="satP"
                     /locus_tag="JNZ73_000728"
     CDS             8169..8735
                     /gene="satP"
                     /locus_tag="JNZ73_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HAY8731118.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8770..9357)
                     /gene="mog"
                     /locus_tag="JNZ73_000729"
     CDS             complement(8770..9357)
                     /gene="mog"
                     /locus_tag="JNZ73_000729"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HAY8731119.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9472..10425)
                     /gene="tal"
                     /locus_tag="JNZ73_000730"
     CDS             complement(9472..10425)
                     /gene="tal"
                     /locus_tag="JNZ73_000730"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY8731120.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10704..12152
                     /locus_tag="JNZ73_000731"
                     /pseudo
     CDS             10704..12152
                     /locus_tag="JNZ73_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12222..12998
                     /gene="yaaA"
                     /locus_tag="JNZ73_000732"
     CDS             12222..12998
                     /gene="yaaA"
                     /locus_tag="JNZ73_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAY8731121.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13151..13447)
                     /locus_tag="JNZ73_000733"
     CDS             complement(13151..13447)
                     /locus_tag="JNZ73_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY8731122.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13661..14947)
                     /gene="thrC"
                     /locus_tag="JNZ73_000734"
     CDS             complement(13661..14947)
                     /gene="thrC"
                     /locus_tag="JNZ73_000734"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAY8731123.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14948..15880)
                     /gene="thrB"
                     /locus_tag="JNZ73_000735"
     CDS             complement(14948..15880)
                     /gene="thrB"
                     /locus_tag="JNZ73_000735"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAY8731124.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15882..18344)
                     /gene="thrA"
                     /locus_tag="JNZ73_000736"
     CDS             complement(15882..18344)
                     /gene="thrA"
                     /locus_tag="JNZ73_000736"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HAY8731125.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            complement(18425..18490)
                     /gene="thrL"
                     /locus_tag="JNZ73_000737"
     CDS             complement(18425..18490)
                     /gene="thrL"
                     /locus_tag="JNZ73_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HAY8731126.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18704..19390)
                     /locus_tag="JNZ73_000738"
     CDS             complement(18704..19390)
                     /locus_tag="JNZ73_000738"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY8731127.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSSDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19790..19930)
                     /locus_tag="JNZ73_000739"
     CDS             complement(19790..19930)
                     /locus_tag="JNZ73_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731128.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20026..20742
                     /gene="arcA"
                     /locus_tag="JNZ73_000740"
     CDS             20026..20742
                     /gene="arcA"
                     /locus_tag="JNZ73_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HAY8731129.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20801..22153)
                     /gene="creD"
                     /locus_tag="JNZ73_000741"
     CDS             complement(20801..22153)
                     /gene="creD"
                     /locus_tag="JNZ73_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HAY8731130.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22211..23635)
                     /gene="creC"
                     /locus_tag="JNZ73_000742"
     CDS             complement(22211..23635)
                     /gene="creC"
                     /locus_tag="JNZ73_000742"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HAY8731131.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     VAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNTIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRNFT"
     gene            complement(23635..24324)
                     /gene="creB"
                     /locus_tag="JNZ73_000743"
     CDS             complement(23635..24324)
                     /gene="creB"
                     /locus_tag="JNZ73_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HAY8731132.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSLGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24337..24810)
                     /gene="creA"
                     /locus_tag="JNZ73_000744"
     CDS             complement(24337..24810)
                     /gene="creA"
                     /locus_tag="JNZ73_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HAY8731133.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25021..25890
                     /gene="robA"
                     /locus_tag="JNZ73_000745"
     CDS             25021..25890
                     /gene="robA"
                     /locus_tag="JNZ73_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HAY8731134.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGKFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25887..26534)
                     /gene="gpmB"
                     /locus_tag="JNZ73_000746"
     CDS             complement(25887..26534)
                     /gene="gpmB"
                     /locus_tag="JNZ73_000746"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HAY8731135.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26586..27107
                     /gene="yjjX"
                     /locus_tag="JNZ73_000747"
     CDS             26586..27107
                     /gene="yjjX"
                     /locus_tag="JNZ73_000747"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
                     /protein_id="HAY8731136.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(27192..27518)
                     /gene="trpR"
                     /locus_tag="JNZ73_000748"
     CDS             complement(27192..27518)
                     /gene="trpR"
                     /locus_tag="JNZ73_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HAY8731137.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27608..29545)
                     /gene="sltY"
                     /locus_tag="JNZ73_000749"
     CDS             complement(27608..29545)
                     /gene="sltY"
                     /locus_tag="JNZ73_000749"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HAY8731138.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     EIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29756..31423
                     /gene="ettA"
                     /locus_tag="JNZ73_000750"
     CDS             29756..31423
                     /gene="ettA"
                     /locus_tag="JNZ73_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HAY8731139.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31731..32963)
                     /gene="nadR"
                     /locus_tag="JNZ73_000751"
     CDS             complement(31731..32963)
                     /gene="nadR"
                     /locus_tag="JNZ73_000751"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HAY8731140.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32984..34366)
                     /gene="radA"
                     /locus_tag="JNZ73_000752"
     CDS             complement(32984..34366)
                     /gene="radA"
                     /locus_tag="JNZ73_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAY8731141.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34415..35383)
                     /gene="serB"
                     /locus_tag="JNZ73_000753"
     CDS             complement(34415..35383)
                     /gene="serB"
                     /locus_tag="JNZ73_000753"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HAY8731142.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAARSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35489..36133
                     /locus_tag="JNZ73_000754"
     CDS             35489..36133
                     /locus_tag="JNZ73_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HAY8731143.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36161..37177
                     /gene="lplA"
                     /locus_tag="JNZ73_000755"
                     /pseudo
     CDS             36161..37177
                     /gene="lplA"
                     /locus_tag="JNZ73_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37209..37472
                     /locus_tag="JNZ73_000756"
     CDS             37209..37472
                     /locus_tag="JNZ73_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8731144.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37633..38352)
                     /gene="deoD"
                     /locus_tag="JNZ73_000757"
     CDS             complement(37633..38352)
                     /gene="deoD"
                     /locus_tag="JNZ73_000757"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAY8731145.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38409..39632)
                     /gene="deoB"
                     /locus_tag="JNZ73_000758"
     CDS             complement(38409..39632)
                     /gene="deoB"
                     /locus_tag="JNZ73_000758"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY8731146.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLIRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39684..41006)
                     /gene="deoA"
                     /locus_tag="JNZ73_000759"
     CDS             complement(39684..41006)
                     /gene="deoA"
                     /locus_tag="JNZ73_000759"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HAY8731147.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLDGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MVLCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41133..41912)
                     /gene="deoC"
                     /locus_tag="JNZ73_000760"
     CDS             complement(41133..41912)
                     /gene="deoC"
                     /locus_tag="JNZ73_000760"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAY8731148.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42170..43711
                     /locus_tag="JNZ73_000761"
     CDS             42170..43711
                     /locus_tag="JNZ73_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HAY8731149.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDTRCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43683..44546
                     /gene="yjjW"
                     /locus_tag="JNZ73_000762"
     CDS             43683..44546
                     /gene="yjjW"
                     /locus_tag="JNZ73_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HAY8731150.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEVTTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44760..45542)
                     /locus_tag="JNZ73_000763"
     CDS             complement(44760..45542)
                     /locus_tag="JNZ73_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY8731151.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45539..46612)
                     /locus_tag="JNZ73_000764"
     CDS             complement(45539..46612)
                     /locus_tag="JNZ73_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAY8731152.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46734..46895)
                     /locus_tag="JNZ73_000765"
     CDS             complement(46734..46895)
                     /locus_tag="JNZ73_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAY8731153.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(47022..47627)
                     /gene="osmY"
                     /locus_tag="JNZ73_000766"
     CDS             complement(47022..47627)
                     /gene="osmY"
                     /locus_tag="JNZ73_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HAY8731154.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKDYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(48020..49609)
                     /gene="prfC"
                     /locus_tag="JNZ73_000767"
     CDS             complement(48020..49609)
                     /gene="prfC"
                     /locus_tag="JNZ73_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAY8731155.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49700..50377)
                     /gene="yjjG"
                     /locus_tag="JNZ73_000768"
     CDS             complement(49700..50377)
                     /gene="yjjG"
                     /locus_tag="JNZ73_000768"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HAY8731156.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50392..50838)
                     /gene="rimI"
                     /locus_tag="JNZ73_000769"
     CDS             complement(50392..50838)
                     /gene="rimI"
                     /locus_tag="JNZ73_000769"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAY8731157.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50807..51220)
                     /gene="holD"
                     /locus_tag="JNZ73_000770"
     CDS             complement(50807..51220)
                     /gene="holD"
                     /locus_tag="JNZ73_000770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HAY8731158.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51323..52354
                     /gene="rsmC"
                     /locus_tag="JNZ73_000771"
     CDS             51323..52354
                     /gene="rsmC"
                     /locus_tag="JNZ73_000771"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HAY8731159.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVITQTGRFKVYRA
                     IMTRQAKKG"
     gene            52631..52717
                     /locus_tag="JNZ73_000772"
     tRNA            52631..52717
                     /locus_tag="JNZ73_000772"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52665..52667,aa:Leu,seq:cag)
     gene            52746..52832
                     /locus_tag="JNZ73_000773"
     tRNA            52746..52832
                     /locus_tag="JNZ73_000773"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52780..52782,aa:Leu,seq:cag)
BASE COUNT        12590 a        14297 c        13267 g        12710 t
ORIGIN      
        1 tatcagtttc atcatataag atttgactgc atccagggcg agcaaggtga attagttttt
       61 gaacccagtg acgttgaata ttactttgaa ccagtgacga tacaggagtc aggaaccagc
      121 atattaaaaa atgagttaga aaacagtgag gacggtgcgg gtcaaatagg ctttcaactc
      181 tccaatgatg gtacgaatga aatccagtat ggtaaaagca attactactc ttttcagcat
      241 ccccatgaag gaagtaatca gattcctcta tttattcgcc cgcgtacata tggaaacaat
      301 gtctcaagcg ggcaaataat gagccgagtc aaaatcgtgg taatgtacaa ctaatctatc
      361 tcccccacct taactttggg ggagctgccg attaacgcac cgctggacta aaaagcgcag
      421 aataatccgt attgcagatt cgctgtaccg ccgggtgctg aatcatccgc tcagcaaaaa
      481 tagcatggta ctcttccatc acattctcga cgcgaccaat ttctacgaca gttttatcgg
      541 cataaaaatc atatgcataa agcgttgggg caacgaagat tgcattgtgc atcgcaccaa
      601 aagctttcat caaagcggca tcatcaaact cgccgaggat ttctacgttt aatccctggg
      661 agttaaacca gttaagcaat ttgcgcccta acattgaacg tcgcccagga atcaaaagtc
      721 gccgttcttc cagacaagcc gggaacggtt tttctggtgg tggatttgta caccagaaac
      781 tcacgccaca ttcgccaatt ctcacggaga acaggccttc ctgctgcgta gagtctatcg
      841 gacagtcaga aatgatcata tccagtttat gctgacttaa ttgctccagc agcatttcgt
      901 gggtggattc gaagcagcga agatgaatgg gctcgccttc taccactgcg gcgttaagta
      961 cgctactgac caggcgtttg gaaagtgcat cagccacgcc aacgtcaaac aataaattgg
     1021 attctttgcg atagttcaca atatccagca tttcctggct taaggtgaac attttatcgg
     1081 catagcgata gaccagttct cccagctcgc tgggttcgag accacgtccc ttgcgtttaa
     1141 ataatttgcc ttgcaggcgc tcttccagcg ctcgaatctg tccggtaatg gtttgtggag
     1201 ttaaataaag cgcctccgct gcgccaacca cggaaccttc tttatagaca tgccagaagt
     1261 aatacaagtg gttgtaattg atatgagaca tgctcatttc tctccctgat aacaatgaaa
     1321 agggagccgt ttatggctcc ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac
     1381 gcgtaaccag ctgtatccaa ttaccgccga agagatagaa ccgaccagga taccgagttt
     1441 cgcccagtta atcagttctg gatctacgct accaaaggcc aggctggcaa taaagataga
     1501 catagtaaaa ccgataccgc acaggatccc caccaccata atttgctgat aagtcgttcc
     1561 ctcaggcaga tgcgccagtt tcaaacgcag cgccaaccag cagaacagac taatccccag
     1621 cggtttgcca atcagcaagc cagcgatgat ccccaatggc agaatggagg tcaagccatc
     1681 cagcgtgacg ccttgcagtg aaacgccagc attagaaaat gcaaacagcg gcaaaatcag
     1741 atacgccacc cacgggtgca acacatgctc cagtcgcttc gctggagaac gcccatgctt
     1801 ctctttcaaa ggaataaaga agccgacaat tacccccgcc agagttgcgt gaacccccga
     1861 tttcaacacc gcagtccaca acaccacgcc aacaagaata tagacgcccg tgcggcgtac
     1921 accacacaga ttcaataccg cgagtaccgc aattgctaca gccgcgacgc caagagaggc
     1981 catcgataag tcattagtgt agaacaatgc gatgataatg atggccccaa gatcgtcgat
     2041 aatagccaga gccatcaaaa agatcttcag cgctaacgga acacgacttc ccaacagcgc
     2101 cagtacacca agtgcaaaag caatgtcagt agccgccggg atcgcccacc cttcgcgggt
     2161 aatcggatcg gcatagttaa aaggcagata gagtaatgcc ggcacaatca tcccaccaat
     2221 agcggcgata actggaaatg cggcctggcg taagctggct agcgatcctt gcatcagttc
     2281 acgtttaact tccagaccga ctaacaggaa aaataccgcc atcagcgcgt catttatcca
     2341 taacagcatg tttttgttga tttcgagtga accaacccgg agctgaaccg gcgtctccag
     2401 aaagtcgtga taccatccac tggttgcgcc gctgttggcc ataatcatcg ccaggatagc
     2461 ggcaatgata agaataatgc ctcccgaggc atcactgcta aagaattgat gcagatgttt
     2521 cactttttat ttctctttca ggtgaataga tcgtttttac tattttaccc cgcacgaatc
     2581 atcgttaaaa tcagattata gtgtaggaat aattcgtttt ttacgagcta ttgaagaatt
     2641 tgcgccatca gcggtgagat gcgtttttga cggctgtaag cgctcacgag tgaccctaag
     2701 cccagataat catagcttaa ggtgaggatt cctgagggat taattaggaa aagtaataat
     2761 taatttgttg tcagtcggcg atgcttgtta ggccaatatt tcaaaaataa tccagcttgt
     2821 cgtcaataat tgcgatgaag agataaatta aagttaaata attaaatcat taaaaaatta
     2881 cgccacaaaa atgcgaggcg tcttcaggat tagaatcgat aaaacaaaat gggttaaaga
     2941 gagcgatata atagcggcgg gtgcttgagg ctgtctgtct caggcattag ctgaacggca
     3001 gatagagaaa agccccgagt gatattttac catcaacccg aggcctccta tatgctgaac
     3061 acatgtagag tgcctcttac tgaccgtaag gtcaaggaga agagagcaat gaagcagcat
     3121 aaggcgatga ttgtcgccct gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggtg
     3181 acgagaaaag acctctgtga ggttcacatc cgaactggcc agacggaggt tgctgttttc
     3241 acggcttacg aatccgagta agagcaacgg cggggagtga tccccgccac tttacaggtg
     3301 ctcgcatatc ttcaacgcac cctattttta cccaggcctg cccacgggca ggcttttggg
     3361 gaggttagcg ggtcaggtcg tcaaaaaact tcttcacacc atcaaagaag ctctttgagc
     3421 gcgggctgtt gtgctcgccg gttgggccac cgaagctttc ttgcagctct tgcagcagct
     3481 gtttctgctt ttcgttcagg cctaccggtg tttcgacgac aacgcggcac agcaaatcac
     3541 cctgtgcacc accgcggaca gacttgacgc ctttaccgcg catacggaat agcttaccgg
     3601 tctgggtttc gccaggcact ttcagtttga cgcgaccatc aagggtcggt acttcgattt
     3661 cgccacccag cgccgccata gcgaagttga tcgggacttc gcaatacagg ttgttgcctt
     3721 cacgctcgaa aatcgggtgc tgtttaacct gaacctgaac gtacagatcg cctgccggtg
     3781 cgccatgctc gcccgcttca ccttcgcccg caagacggat gcggtctcca gtgtccaccc
     3841 ctgccgggat tttaacggac agcgttttgc tgcgctcaac acgaccatga ccatgacatt
     3901 tgttgcacgg atctttgatc agcgtaccgc ggccctgaca gtgtggacag gtctgctgta
     3961 cagcgaagaa tccctggcgc atctgcacct gaccagaacc atgacaggtc ggacaggtct
     4021 gcggctgtgt acctggtttt gcaccgctac cgtggcaaac gtcacactct tccagagtcg
     4081 gaatgcggat ttctttggtc acgccacgta cagcttcttc gagggtgagc tccatgttat
     4141 agcgtaaatc agcaccgcgc gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc
     4201 cgaaaacgtc accaaaaata tcgctgaagt ctgcgccgcc gccaaaacca ccgccgccca
     4261 tgcctccttg ctcaaacgca gcatgaccat actgatcgta tgccgcacgt ttttgcgagt
     4321 cggtcagaac ttcataagct tccttgatct ctttaaattt cgcctcggcc tctttgtcac
     4381 cctggttacg gtccgggtgg tatttcatgg ccaggcgttt gtaggccttt ctgatttcac
     4441 gctcttccgc tgttttggaa acgcctaaaa tctcgtaata atcttgctta gccatctttt
     4501 ttaaattgcc cctagatgaa tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt
     4561 ataattaccc gtttataagg cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg
     4621 acaacatcgt catctttcgc gttgtttgca gaagcatcag caccggcagt ctgctgctgg
     4681 gcatgttgct gctgggcgat ttccatcagt ttctgggaaa cctgtgccag ttcctgcatt
     4741 ttcgcttcga tagcggcttt gtcttcacct ttcagagcag tttccagtgc agtcagcgca
     4801 gactcgatag cagttttgtc gtcagccggc agtttgtcgc ctgcttcttc aacctgctta
     4861 cgggagctgt gcagcagatg gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta
     4921 cggtcagctt cggcgttagc ttctgcgtcg cgtaccattt tctggatttc atcttcgttc
     4981 agaccagaag aagccttgat ggtgatcttc tgctctttac cgctgttttt atctttcgcg
     5041 gaaacgtgca ggataccgtc agcatcgata tcgaaggtaa cttcgatctg cggcatgccg
     5101 cgcggtgccg ggttgatacc atctaggttg aactgaccca gagatttgtt atcagccgca
     5161 cgtttacgtt caccctgcag cacatggatg gttaccgcag actggttgtc ttcagcggta
     5221 gagaacacct ggctgtgctt ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc
     5281 acaccgccca tggtttcgat acccagagac agcggggtaa cgtccagcag cagtacgtct
     5341 tttacgtcac cagtcagaac accaccctga acagcagcac cgattgctac agcttcgtcc
     5401 gggttaacgt ctttacgcgg ctctttacca aagaactcag caactttctt ctgaaccatt
     5461 ggcatacgag tctgaccacc aacgaggata acgtcgtcga tatcagatac ggacaggcca
     5521 gcatcctgca gtgcaacttt cagcggctca atggaacggt ttaccagatc ttcaaccagg
     5581 ctttccagtt tcgcacgagt cactttgatg ttcatgtgtt tcggaccggt cgcgtctgca
     5641 gtgatgtatg gcaggttaac gtcggtctgc tgagcggaag acagttcgat tttcgctttt
     5701 tctgccgctt ctttcaggcg ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga
     5761 tctttcttga attcttcaac cagatagttg atcagacggc tgtcgaagtc ttcaccaccc
     5821 aggtgggtat caccgttggt tgccagaact tcgaaggttt tttcgccgtc aacttcgtcg
     5881 atttcgataa tagaaatatc gaaagtacca ccccccaggt cataaaccgc gatagtacgg
     5941 ttgccagtgc ctttgtccag accgtaagcc agcgcagctg cggtcggttc gttgatgata
     6001 cgttttactt ccagaccagc gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg
     6061 ttaaagtatg ccggtacggt gataacagct tcagttaccg gttcacccag gtaatcttca
     6121 gcggttttct tcattttttt cagcacttca gcagaaatct gcggcggtgc cattttctgg
     6181 cctttaactt cgacccatgc gtcgccgtta tcagcagcaa taattttgaa cggcatgatg
     6241 gaaacatcac gctgtacttc ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca
     6301 aacagggtgt tttgcgggtt cgtcactgcc tgacgtttag ccggctgacc aaccagagtt
     6361 tcaccatcct gggtataggc aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc
     6421 agcacgcgag gaatggtgcc atccataatc gctacacaag agttggtagt acccaggtcg
     6481 ataccaatta ttttacccat ctaaacgtct ccactatata ttcggtcatc atgtggttgt
     6541 gagtctgtaa taggggcgaa acgtctggtt tcaactgccc aatttcattt ttttgcagac
     6601 tcactcactg cggttgacta ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc
     6661 aaaaattttt tgtgcttttg cgcagaaatt tcgcgatatg gcaggacaag acgtaaaatt
     6721 gacagcaatt ttattcatat aaagaatgaa tcgcagccaa ggcattcatc aaaaaattgt
     6781 aataaaaaga aaagattacg tgcctgaatc ttctctttat cagcagtaaa ctagtgggta
     6841 ttcatccccc tacctcttcc cactaagaga atccttatga aatccgtttt tacgatttcc
     6901 gccagcctgg cgattagcct gatgctgtgc tgcacggcgc aggcaaacga ccataaactc
     6961 ctcggcgtca ttgcaatgcc gcgtaacgaa accaacgatc tggcgctgaa acttcctgtt
     7021 tgtcgcattg tgaaacgcat acaactctct gccgaccatg gcgatttaca gttaagcggt
     7081 gcatcggttt atttcaaagc cgcccgtagc gccagtcaga gcctgaatat tccttcagaa
     7141 ataaaagaag ggcaaaccac tgactggatc aacattaaca gcgataacga caataaacgc
     7201 tgcgtctcaa aaatcacctt ttccggtcat acggtgaact catcggatat ggccacgctg
     7261 aaaattatcg gcgacgacta acgccagaat atagaagcca caaaaaatga atgttaatta
     7321 cctgaatgat tcagatctgg attttcttca gcattgtagt gaggaacagt tggcaaattt
     7381 cgcccgattg ctcacccata atgaaaaagg caaaactcgc ctctccagcg tactgatgcg
     7441 caacgaactg tttaaatcga tggaagggca tcccgagcaa catcgccgca actggcagct
     7501 gattgccgga gaattacagc attttggtgg cgatagtatc gccaacaaac tgcgcggaca
     7561 cggtaaattg tatcgggcca ttttgctcga tgtttcaaag cgattgaagc tgaaagccga
     7621 caaagagatg tctacgtttg aaattgagca acagttactg gaacaatttc tgcgtaatac
     7681 ctggaagaaa atggacgagg aacataagca ggagtttctg cacgcggtcg atgccagggt
     7741 gaatgagctg gaagagctgc tgccgctgct gatgaaagac aaattattgg caaaaggtgt
     7801 gtcgcatttg ctttccagcc aactgacccg cattttacgc acccacgcag caatgagcgt
     7861 acttgggcat ggtttgctgc gcggagcggg gctgggaggc cctgtgggcg cagcattaaa
     7921 tggggttaaa gcggtcagcg gcagcgccta tcgcgtgacg attccagccg tactgcaaat
     7981 cgcctgcctg cgccggatgg ttagcgccac tcaggtctga taccggaaac cggggaaaat
     8041 tttcattttc ccctcaaaag atcgtagaca ctgccccact ggctgattat tatgccgcgc
     8101 cctgaaaaca ctacagttat tcagggaaat tatttcacca ttcattcgat gatgattttt
     8161 gaggaattat gggcaacact aagttggcta atccggcacc gctgggcctg atgggcttcg
     8221 gcatgaccac cattctgctt aacctgcaca acgtgggtta tttcgctctg gacggtatta
     8281 ttcttgccat gggcattttc tacggcggca tcgcgcaaat ttttgctggt ctgctggagt
     8341 acaaaaaagg caacactttc ggtttaaccg cattcacctc ttacggttct ttctggctga
     8401 cgctggttgc gattctgctg atgccgaaac tgggtctgac cgatgcgcca aatgcacagt
     8461 tccttggtgt ctacctgggt ctgtggggcg tatttacgct gtttatgttc ttcggcactc
     8521 tgaaaggcgc acgcgtcctg caattcgttt tctttagcct gaccgtgctg tttgccctgc
     8581 tggcgatcgg taacattgcc ggtaatgccg cgatcatcca ctttgccggc tggattggtc
     8641 tgatctgcgg tgccagcgca atctatctgg cgatgggtga agtgctgaac gagcagtttg
     8701 gtcgcaccgt tctgccgatt ggtgaatccc actaattgtc ttgagatccc cccgctcggg
     8761 gggatttttt tattcgctaa cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac
     8821 ttctggtgcc gtttcaacgt atggcccttc cagcaactga atgcagtacg gtacgctggc
     8881 aaaaatacca tgcaccacaa cgttaccctc agcgtccttc acaccttcca gcgtctcttt
     8941 aatagacttc ggctgaccgg gtaagttaag gatcagcgcc tgtttgcgaa tcacgcccac
     9001 ctgacgcgaa aggatcgcag ttggtacaaa atgcaggctg atctggcgca tctgttcacc
     9061 aaagccaggc atctcgcggt ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg
     9121 cgccgggcca gttccgcccg tggtgagcac cagatggcaa ctcatttcat ccaccagctc
     9181 acacaacgtt tgctcgatga tcgcctgctc atcggggatt aagcgggttt ccagttcaaa
     9241 cggcgtggtt agcgccgatg tcagccattc ttccagcgct ggaatgcctt tatcctgata
     9301 aacgccgctg gatgcgcgat cagagatgga aactaagcca atacgtaaag tattcatcaa
     9361 gatttttccg tttaaacagt ctcgttaaac agaatgatac actgcgaagg gagtgacaga
     9421 caggcttcag aagaggtagc gtgaccgact tcccggtcac gctaagaatg attacagcag
     9481 atcgccgatc attttttcca gtttttcctg gtcaatagca aacttacgga taccttccgc
     9541 cagtttatct actgccattg gatcctggtt gtgctgccac aggaactcgg actcagtgat
     9601 acgcgccgga cgcgctttca cttcgccggt gtaagacagt ttacgttcga tagccccttc
     9661 gctctccgcc agctctttca gcagtgccgg tgcgatggtc agacggtcac agcctgccag
     9721 ttccagaatt tcgccgatgt tacggaagct tgcgcccata accacggttt cataaccgtg
     9781 ctctttgtag tactggtaga tttcagatac agaaaccacg cccggatctt ctgccggagc
     9841 gtactctttc ttatcggtat tcgctttgta ccagtcaaga atacggccaa caaacggcga
     9901 gatcaggaac acgcccgcct ccgcacaagc acgagcctga gcgaaggaga acagcagggt
     9961 caggttacag ttgatacctt ctttttccag ctgttctgca gcacggatac cctgccaggt
    10021 agaagccagt ttgatcagaa tacgatcgtt gctaatacct gcatcgttgt agagtttgat
    10081 caggcgtttt gctttcgcaa ttgacgcttc ggtgtcatag gaaagacgcg catcaacttc
    10141 agttgagata cggcccggaa ccagtttcag gatttccaga ccaatattta ctgccagttt
    10201 gtcggtcgcg tccacgatct gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac
    10261 agcatcatca atcaacttac ggtattccgg aatctgcgct gcgttaagaa tgagagaagg
    10321 gttggttgtg gcatcctgcg gttgatacag cttcattgcc gcgatgtccc cagtgtcggc
    10381 cactacggtg gtgtactgac gaagggaggt caatttgtcc gtcatgatag tatttctctt
    10441 taaacagctt gttaggggga tgtaaccggt ctgccctgat gatatcacga cgcaacgtca
    10501 gcgcaaccgc cgacaagacg gttatcgcca ggcgagactg tttcggattt ctgacactgc
    10561 gttgatatcg cccgccattt ttatacaaaa cctcatgtat gctacgcaga agttatcaag
    10621 tacctcgtag cgtatatact tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc
    10681 gaccggcaat aagagggata tgcatgccag attttttctc ctttattaac agcgtccttt
    10741 ggggatcggt aatgatttac ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg
    10801 gatttgtgca gtttcgctac atccgccagt ttggcaaaag tcttaaaaat agcattcatc
    10861 cacagccagg cggtttaacc tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg
    10921 gtagcggcaa cctggccggc gttgcgctgg caattaccgc cggtggacca ggtgccgtct
    10981 tttggatgtg ggttgctgcg tttatcggca tggcgacctc gtttgccgaa tgttcccttg
    11041 cacaacttta taaagaacgt gacgtcaatg ggcagtttcg tggcggaccg gcatggtata
    11101 tggcgcgcgg gctggggatg cgctggatgg gcgttctgtt cgccgtcttt ttgctcatcg
    11161 cctacggcat aattttcagc ggagttcagg cgaacgccgt tgcccgagcc ctgagttttt
    11221 cttttgattt tcctccgctg gtgacgggca ttattctcgc tgtctttgct ctgctggcaa
    11281 tcactcgcgg tcttcatggc gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga
    11341 taatctgggt actgaccagc ctggtgattt gcgtaataaa tattgggcaa cttccccacg
    11401 tcatttggtc tatttttgag agtgcttttg gctggcagga agcggcaggc ggcgcggcgg
    11461 gatatacctt aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg
    11521 cgggaatggg atcgacgcca aacgcggcag cggcagcggc agcggcagcg gcagcggcat
    11581 cctggcctcc gcatccggca gcacaaggga ttgtccagat gattggcatt tttatcgaca
    11641 ccctggtcat ctgtacggca agcgccatgc tgatattact ggcgggtaac ggcacaacct
    11701 acatgccgct ggaaggtatt cagcttatcc agaaggcgat gcgggtgcta atgggttcct
    11761 agggtgctga atttgttacc ctcgtggtta ttctgtttgc cttcagctcc atcgttgcca
    11821 actacattta tgccgaaaac aatctcttct ttttacgcct gaacaaccct aaagcgatct
    11881 ggtgtttgcg gatctgcacc ttcgcaacgg tcatcggcgg caccttgcta agccttccgc
    11941 tgatgtggca actggcagat atcataatgg cctgcatggc tattaccaat ttgaccgcca
    12001 ttttactgct ctcgcctgtg gttcatacca ttgccagtga ttatctacgc cagcgtaaac
    12061 tcggcgtgcg cccggtgttt gatccgttgc gttatccgga tatcggtcgc cagctttctc
    12121 cggacgcgtg ggatgatgtt tcgcaggagt aatcacaact atcgatcaac tcattctctt
    12181 tttttgctaa agtcggcata aatttcctgc aaggactgga tatgctgatt cttatttcac
    12241 ctgcgaaaac gcttgattac caaagcccgt tgaccaccac gcgctatacg ctgccggagc
    12301 tgttagacaa ttcccagcag ttgatccatg aagcgcggaa actgacgcct ccgcagatta
    12361 gcacgctgat gcgcatcagc gacaaactgg cgggtatcaa cgccgctcgc tttcatgact
    12421 ggcagccaga tttcacgccg gcgaatgccc gccaggcgat tctggcgttt aaaggtgatg
    12481 tctacaccgg cttgcaggcc gaaaccttca gcgaagacga tttcgatttt gcccaacagc
    12541 atttgcgaat gctttccggc ttgtatggcg tactgcgccc gctcgattta atgcagcctt
    12601 atcgtctgga aatggggatc cgtcttgaga atgcccgagg gaaagatctg tatcaattct
    12661 ggggagatat catcaccaac aagctgaacg aggcgctcgc agcacaaggc gataatgtgg
    12721 tgattaatct ggcgtcagat gagtatttta aatcggtgaa gccgaagaaa ttgaatgccg
    12781 agattatcaa gccggtgttc ctcgatgaga agaacggcaa atttaagatc atcagcttct
    12841 acgctaagaa agcacgcggt ctgatgagtc gtttcattat tgaaaatcgg ctgaccaaac
    12901 cagagcaact gactggtttt aatagcgaag gttacttctt tgatgaagat tcctccagca
    12961 atggcgaact ggtgtttaaa cgctacgagc agcgttaagt ttgtagtcaa tgccggatgc
    13021 ggcgtgaacg ccttatccgg cctacaaaag catgcagatt caatatattg cagaattaac
    13081 gtaggcctga taagacgcgc cagcgtcgca tcaggcgctg aatgccggat tgttacccgg
    13141 catttgtcat ttagcggtga tgtttgcctg gaccatgacc gccgtgatga tcatgaggag
    13201 ctttcttatg gtggcgcggc ggtggcggcg gtccgtgtgg gtgccagcga ttgcctcgcc
    13261 attcataatg ttgtttccac cagccgtggt cgcgccagtg acctccatcc cagtaatagc
    13321 cacgattatc acgatcgcct atctgtaatt ttactgacgg gactaacgta atttccgcag
    13381 cctgtgctgc catgggagcg accagaacca gggaaagtgc gagtacgata gattgcatct
    13441 ttttcacagg ttactccttt tatcattgcc cgttatgggt cgatatccgg agattacgcg
    13501 atgagaacga cggttgttat tctccgcaat cctaatctct aagttaccgc atttattgat
    13561 aatttctcct ttttccctgt cgtttttctc cataatttct tcataaaaaa gccgggctgc
    13621 ataaaagcaa acccggcctg attgagataa tgaatagatt ttactgatga ttcatcatca
    13681 atttacgcaa cgcagaaaaa tcggcgggca gattatgtga aagcaagggt aaatcagcac
    13741 gttctgccag ctcttttggc agatccaacg tttcaccgag aatcgcttcc acgctctctt
    13801 taaatttcgc cggatgcgcg gtgccgagga acaagccata ttcgcctgga ttcaactggt
    13861 cacgcagcgc acgatacgct acggcagcgt gcggctccga ggtgtagccc agttctttta
    13921 actcacgcat tgtctgttgc gtggtttcat catccacggc tgcataaccc agctctttca
    13981 gttgccagat tttgcggcgg aacaactctt ccacacgcgg ccagttgttc ggctgactca
    14041 cgtccatcgc gttggataac gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca
    14101 ggaaacgtgg cacggtatcg ttcacgttgg tcgcagcaat aaaacgtttc accggcagac
    14161 cgaatgactt cgccagcaga cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga
    14221 caaccagctg gttgcgcgcc tcctgcggca gctgcgcaac agcttcaaag tagtagcaaa
    14281 tctgcgccag caaacggctg atgttaatcg agttagccga gtttaacccc agcgccactt
    14341 tcagttcttc atcatcaaac gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt
    14401 cgatggcaac cgtttcgata ttgccgccca acgtacagaa cagtttttct tgcagtggac
    14461 tgattttgcc tcgtggatag aggataacca ctttcacatt cggcaaaccg tagaaagcat
    14521 gagccactgc agctccggta tcaccggagg tcgcggtcag aatggtcact ggcttatcat
    14581 ccgcaatatg ggtcagcatt tgtgccataa agcgaccgcc gaaatcttta aatgccagcg
    14641 ttggcccgtg gaacaattcc agacaaccga catcgctttc aacattggcg accggagccg
    14701 ggaaggcaaa cgccgcgcgc acgcgctctt ccaggatttc ctgtgggatt tcatcaccaa
    14761 taaacgccga gaggatcttc gcactgcggg tgacaaaatc cagcttcagc atctcatcaa
    14821 tttcagtcag gctgaattcc ggcaggtcgt gcggaaaaaa cagcccctga tttttgccca
    14881 acccctgggt tacggcttgc gcaaagctga cctgctcgtt gtgatctttc agattgtaga
    14941 gtttcattta gttttccagt actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa
    15001 aaccttcctg attttgcagg tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat
    15061 ccggcttgtc acatagagcg aacaaggtcg ggccggagcc ggagataccg ctcgctaccg
    15121 cgccgatttc cgcgaccgcc tgccgcgcct gccggaagcc aggcagtaac cgttcacggt
    15181 agggttcagc gataacatct ttcatcagct tcgcggcaag ctcaggctga cgggaatagc
    15241 aggcgtgaat gaagccagcc agatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact
    15301 gcgccggtaa aatagcccgg gcttctgccg tcgagacttt aattcccgga tacgccagca
    15361 cccacagcca ctcatcaaac cctggcactt gctggctgat gatgtcgttt tcttcgatca
    15421 tcaactgcat accaccaaga aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga
    15481 tacgtccttc cagctcgccc atcaaagcca gcaaacgagt gtcattaagc ggcttgccgc
    15541 agtgttcatt catcgccatc agcgccgcga ccaccgaaca ggcgctggag cctaagcccg
    15601 aaccgatcgg catattcttt tccagagtca tcgccactgg aatttgcttg ccaagctcct
    15661 ggcaaaaacg ctcccagcac tgataaacga tattttcccg tggttctgac ggcagcttat
    15721 cggcaaagcg tccgaggttg ttgagactga atgtctctgc cgcctcaacc gtgactacat
    15781 ctccgagcaa tgcaccatca acgggtgcca ccgccgcccc gagcacatca aacccgacgc
    15841 tcatattggc actggaagcc ggggcataaa ctttaaccat gtcagactcc taacttccat
    15901 gagagggtac gtagcagatc ggcaaagaca ccggcagcgg taacgtcatt gcccgcacca
    15961 tatccgcgca gcaccaacgg cagcggctga taatagtggc tataaaaggc cagggcgttt
    16021 tcgccatttt tcactttgaa cagcggatca ttcccatcca cttcggcaat cttcacgcgg
    16081 cagacaccat cttcatcaat attgccaaca tagcgcaaaa cttttccttc atcacgggcc
    16141 ttcgccacgc gcgcggcaaa gagatcgtcg agctgtgaca gattcgccat aaaagcggca
    16201 acatcaccct cggcgttaaa ctctgcgggc agcacaggtt caatttcaat atccgccagc
    16261 tccagttcac gtcccgtttc acgagcgaga atcaatagtt tacgcgccac atccatacca
    16321 gaaagatcat ctcgcgggtc cggttcggta taacccattt cccgcgccag cgtggtcgcc
    16381 tcggagaaac tcatgccttc gtctaacttg ccgaagatat aagaaagcga acctgaaaga
    16441 atgccggaga acttcaccaa ttcatcacct gcattgagca gattttgcag gttctcaata
    16501 accggtaatc cagccccaac gttggtgtca tagaggaatt tacgccgcga tttttccgcc
    16561 gcataacgca actgatgata gtaatccatc gacgaggtgt tggccttttt gttcggcgtg
    16621 acaacgtgga aaccttcgcg caggaagtcg gcatattgat ccgccactgc ctggctggag
    16681 gtgcagtcaa caatgaccgg gttcagcaga tgatattctt tcacgaggcg aattaagcgc
    16741 ccgagattaa acggctcttt ggcttgtgca agttcttcct gccagttttc cagattaagg
    16801 ccatgcacac tagtgagcag agccttcgag ttggcaacgc cgcagacacg taagtcgata
    16861 tgtttattct tcagccagct ttgctgacgc ttcagttgct ccagcagcgc accgccaacg
    16921 ccaccgacgc caatcacaaa cacttcgata acctgatcgg tattgaacag catctgatga
    16981 gtaacgcgca cgccagtggt cgcatcatcg ttatttacca cgacagagat tgagcgttca
    17041 gaagatccct gagcaatggc gacaatgttg atattggcgc gggccagcgc ggcaaagaat
    17101 ttcgccgaga tcccacgcaa ggtgcgcata ccatcaccta ccaccgagat aatggccagc
    17161 cgttccgtca ccgccagcgg ctccagtaag ccttctttca gttccaggta gaactcttcc
    17221 tgcattgccc gttcagctcg cacacagtcg ctttgtggaa cgcagaaact gatactgtat
    17281 tcggaagatg attgcgtaat cagcaccacg gaaatacggg cgcgtgacat tgcagcaaag
    17341 acgcgcgccg ccatgccaac catccctttc atccccgggc cggaaacgct gaacattgcc
    17401 atgttattca gattggaaat gcccttgacc ggtaattcgt cttcatcacg gctggcacca
    17461 atgagcgtac ctggtgcttg aggatttccg gtatttttaa tcaggcaagg gatctggaac
    17521 tgggcgatgg gggtaatggt gcgggggtga agaactttag cgccgaagta ggaaagctcc
    17581 atcgcttcct ggtaggacat cgacttcaac aacctcgcat cgggcacctg acgcgggtcg
    17641 caggtataga ccccgtcaac gtccgtccaa atctcgcaac aatcggcgcg taaacaggca
    17701 gccagcaccg ccgcggagta gtcggaaccg ttacgtccaa gcaccaccag ttcgcctttt
    17761 tcattaccgg cggtgaaacc tgccatcaac accatgtgat cagccggaat gcggcttgcc
    17821 gcaatacggc gggtggactc agcaatatcg acggtagatt cgaggtaatg ccccactgcc
    17881 agcagttttt cgaccggatc gataacggta acgttgtgac cacgcgcttc taacacgccg
    17941 gccataatgg cgatcgacat tttctcgcca cggcaaatca gcgcagcgtt gatgctatcc
    18001 gggcactgcc ccaacaaact aatgccatgc aggacatgtt ttatttgggc aaattcctga
    18061 tcgacgaaag ttttcaattg cgccagcggg aaccccggct gggcggcggc gagtcccgtc
    18121 aaaagttcgg caaaaatacg ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta
    18181 atggtttttt caatcatcgc caccaggtgg ttggtgattt tggcgggggc agagaggacg
    18241 gtggccacct gcccctgcct ggcattgctt tccagaatat cggcaacacg cagaaaacgt
    18301 tctgcatttg ccactgatgt accgccgaac ttcaacactc gcatggttgt tacctcgtta
    18361 cctttggtcg aaaaaaaagc ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta
    18421 cgcgtcagcc cgcaccgtta cctgtggtaa tggtgatggt ggtggtaatg gtggtgctaa
    18481 tgcgtttcat ggatgttgtg tactctgtaa tttttatctg tctgtgcgct atgcctatat
    18541 tggttaaagt atttagtgac ctaagtcaat aaaattttaa tttactcacg gcaggtaacc
    18601 agttcagaag ctgctatcag acactctttt tttaatccac acagagacat attgcccgtt
    18661 gcagtcagaa tgaaaagctg aaaaatactt actaaggcgt tttttatttg gtgatatttt
    18721 tttcaatatc atgcagcaaa cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca
    18781 ggcgttgctg taaccagtcg accagtttta tgtcatctgc cactgccaga gtcgtcagca
    18841 atgccatgac tcgttcgcgt aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt
    18901 tcgccggttg ttgtattaat gttgctaatt gatagcaata gaccatcacc gcctgcccca
    18961 gattgagcga aggataatcc gccaccatcg gcacaccagt aagcacgtca gccaacgcta
    19021 actcttcgtt agtcagcccg gaatcttcgc gaccaaacac cagcgcggca tggctcatcc
    19081 atgaagattt ttcctctaac agcggcacca gttctactgg cgtggcgtag taatgatatt
    19141 tcgcccgact acgcgcagtg gtggcgacag tgaaatcgac atcgtgtaac gattcagcca
    19201 gtgtcgggaa aactttaata ttatcaataa tatcactaga tccatgtgcg acccagcggg
    19261 tggctggctc caggtgtgcc tgactatcga caatccgcag ttcgctaaaa cccatcgttt
    19321 tcattgcccg cgccgctgcc ccaatatttt ctgctctggc gggtgcgacc aggataatcg
    19381 ttatacgcat attgccactc ttcttgacca aataaccgcg aacagggtga tcactgtcaa
    19441 cttattacgc ggtgcgaatt tacaaattct taacgtaagc cgcagaaaaa gccctttact
    19501 tagcttaaaa aaggctaaac tattttctga ctgcactaac ggtttaattg ttaaaaaatg
    19561 ctacatatcc ttctgtttac ttaggataat tttataaaaa ataaatctcg acaattggat
    19621 tcaccacgtt tattagttgt atgatgcaac tagttggatt attaaaataa tgtgacgaaa
    19681 gctagcattt agatacgatg atttcatcaa actgttaacg tgctacaatt gaacttgata
    19741 tatgtcaacg aagcgtagtt ttattgggtg tccggcccct cttagcctgt tatgttgctg
    19801 ttaaaatggt taggatgaca gccgtttttg acactgtcgg gtcctgaggg aaagtaccca
    19861 cgaccaagct aatgatgttg ttgacgttga tggaaagtgc atcaagaacg caattacgta
    19921 ctttagtcat gttacgccga tcatgttaat ttgcagcatg catcaggcag gtcagggact
    19981 tttgtacttc ctgtttcgat ttagttggca atttaggtag caaacatgca gaccccgcac
    20041 attcttatcg ttgaagacga gttggtaaca cgcaacacgt tgaaaagtat tttcgaagcg
    20101 gaaggctatg atgttttcga agcgacagat ggcgcggaaa tgcatcagat cctctctgaa
    20161 tatgacatca acctggtgat catggatatc aatctgccgg gtaagaacgg tcttctgtta
    20221 gcgcgtgaac tgcgcgagca ggcgaatgtt gcgttgatgt tcctgactgg ccgtgacaac
    20281 gaagtcgata aaattctcgg cctcgaaatc ggtgcagatg actacatcac caaaccgttc
    20341 aacccgcgtg aactgacgat tcgtgcacgc aacctgctgt cccgtaccat gaatctgggt
    20401 actgtcagcg aagaacgtcg tagcgttgaa agctacaagt tcaatggttg ggaactggac
    20461 atcaacagcc gttcgttgat cggccctgat ggcgagcagt acaagctgcc gcgcagcgag
    20521 ttccgcgcca tgcttcactt ctgtgaaaac ccaggcaaaa ttcagtctcg tgctgaactg
    20581 ctgaagaaaa tgaccggccg tgagctgaaa ccgcacgacc gtactgtaga cgtgacgatc
    20641 cgccgtattc gtaaacattt cgaatctacg ccggatacgc cggaaatcat cgccaccatt
    20701 cacggtgaag gttatcgctt ctgcggtgat ctggaagatt aatcggcttt accaccgtca
    20761 aaaaaacggc gctttttagc gccgttttta tttttcaacc ttatttccag atacgtaatt
    20821 catcgtccgt tgtaacttct ttactggctt tcattttcgg cagtgaaaac gcataccagt
    20881 cgatattacg agtcacaaac atcatgccgg ccagcgccac caccagcaca ctggttccca
    20941 acaacagcgc gctatcggca gagttgagca gtccccacat cactccatcc agcaacaaca
    21001 gcgcgagggt aaacaacatg ctattgcgcc agcctttcaa taccgcctgc aaataaatac
    21061 cgttcattaa cgccccaatc agactggcga ttatccatgc cacggtaaaa ccgatatgtt
    21121 cagaaagcgc cagcaagagc aaataaaaca tcaccaatga aagccccacc agcaaatatt
    21181 gcattgggtg taaacgttgc gcggtgagcg tttcaaaaac aaagaacgcc ataaaagtca
    21241 gtgcaatcag cagaatggcg tacttagtcg cccggtccgt taattggtat tgatcggctg
    21301 gcgtcgtcac tgcgacgcta aacgcgggga agttttccca gccggtatca ttgcctgaag
    21361 caaaacgctc accgagatta ttagcaaacc agctgctttg ccattgcgcc tgaaaacctg
    21421 actcgctagc ttcccgtttg gctggtagaa aatcacctaa aaaactggga tgcggccagt
    21481 tgctggttaa ggtcatttcg ctattacgcc cggcaggcac cacagaaaga tcgccggtac
    21541 cgcttaaatt cagtgccata ttcagctgca ggttctgctt ccgccagtcc ccttcgggta
    21601 aagggatatg cacgccctgc ccgccttgct ctaacccggt accgggttca atggtcagtg
    21661 ccgttccgtt aacttcaggc gctttcacca caccaatacc acgcgcatcc ccgacgctaa
    21721 tcacaataaa gggcttgcct aagatgatat ttggcgcgtt gagctcgcta agacgcgaaa
    21781 catcgaaatc ggcttttagc gttaaatcac tgtgccagac ctgaccggta taaatcccta
    21841 tcttgcgttc ttccacgttc tgattgccat caaccatcaa tgactcaggt aaccaaaaat
    21901 ggataaaact tcgtttccgc tccacggttt tatcctcttc ctgcaccgta taaagctcgg
    21961 tcacaggaat agcgataagc ggcccaacga gtttttgcgg cccgctggta ctttggcgaa
    22021 tcgcatcttc cacatcgcta cggtaatcgg cacgttcgac aatcacttgc cgaatcagca
    22081 ttatcggaat caacaacagc aatactgcac caaacaaggt ggtcattttc cagaacaggg
    22141 gggatttcaa catagtcttc tcctttgcaa tggcagcagg atacgaaggc tatgtgggaa
    22201 gaatttgaag ctatgtgaag ttacggtgaa gtcgtagcga ggccagcacg ccaccttcct
    22261 gcacgttgcg cagcgtgact tcgccgttaa acaaacgggc gacctcactg acgaacgcca
    22321 accccagacc gctgcttttt tgcccatttg cacgcggcaa agagtaaaag cgttcaaaaa
    22381 tacgtgacag cgcgtagtca ggaataccac taccggtatc cagcacctta agcgtgacgt
    22441 gttcctgatc cacttcggcg cttagcgtta tgcaaccgct ctcgggagta aaatcgatgg
    22501 tgttatccag taaattcccc agcgcctgat ccagtaacgc cggttcagca gcaacattaa
    22561 cctcggtggg cataacatgc aaagtgatgt ttttttctgc caactgcacg gtgcgcgctt
    22621 cgctgacgcg gcgaaataat gccgccacat caacagcagt cagaacgact tcctgacgat
    22681 tctccagtct tgcctggcgt agtaacgttt ccaccagcgc ctgcattcgc gcattttgtg
    22741 tcagaatgtt gtcagtaaaa cgagccacta cttcaggcgg cggaccttcg cgtaaaattt
    22801 ccgccacgcc acgaatcgcc gccagtgggc tttttagctc atgggttaac gcataaacat
    22861 actgctcaat atagtttttc ccttccagct tcacgcgcat actttccagc gcctgcgcga
    22921 gtttacgcag ctcgctacta ccgagatcgg gaagaggaac gggcttattg tcagtgacgg
    22981 aatcagcata gcgagtgagc cgggcaatgg agcggttgat ccaccaaacc atgcctgcgc
    23041 caatcaccag tgcaatcccc aacaaaatgg cgctggccca taaaattcgc cgctcgctgc
    23101 gtttaatgac cggagccatc gccgcgttcg gtttgcctac gctcaaaacg ccaataagcc
    23161 gcgagccgcc cataatcggc gcggcaacat acatcaccga actttcggga tcggcaggat
    23221 tttgcaacgt gctgcgcgca ccatactgac cacgcaacgt tagccagacg tcattccagc
    23281 gcgaataatc ctgtccaacg gctttatttg ccgaatcgaa caatactttg ccatgcgcat
    23341 cggtcatata gacatgatat tcattgcgca ctttgttaat gccaccaata ttggcgcgaa
    23401 acgggcgatg ttgtagctga ttaaacgcct gcgccagttg cccatgcgtt gggtccccag
    23461 agagcaaatc gggacgcgcc agctccgcaa gtaacgttgc cgtgtcgatc aacgtcccct
    23521 ccgttgctct tcgcacgccc ggtttaactt ctttgacaaa aatagccagt acgaaccagg
    23581 ccgccaccgc caccagtaaa aaatagccca gcagcaaccg catgccgata cgcattacaa
    23641 gcccctcagg ctatatccca tgccgcgatg agtattaatc ggtgaaagat cggggttgat
    23701 ggcgcgcagc ttggcacgca gcgttttaat gtggatatcg acggtgcgat cgtaggtgtc
    23761 ctgcgcatct tcccacacgc tatccattag ttgctggcgg gaccagacgc ggcctagcga
    23821 cttgagtaac gtcttcagca ataaaaactc ataccgcgtc agcgttaatg gcgtgtcaaa
    23881 ccagctgatc tgcgccgcgg gttcattcaa ttcaaaatga ccaatacgga tgacgggaga
    23941 cggcgtcgag aacttcttca cccgacgcag taaggtgcgc accctggcgc acacttcgcg
    24001 gggtgaaaac ggtttagcca catagtcgtc agcgccaatt tccagcccaa gcaggcgatc
    24061 gacctcttca cttcgggccg tcaggaacag tacaggtaac gccggatgga gcgccagtaa
    24121 ctggcggcac aattcaaagc cactgatatc aggcaaacca acgtcgagaa tcatgacgtc
    24181 gggaacctgc tggcgagctt tatccagcac cggcaaacct cgctcaaaga cctcgacggc
    24241 aaaaccttcc tgctgcaaca tgtagaccag cgtgtcggct atcccttgct catcttccac
    24301 taaccagacc gtttcccgtt gcataaatcg cctctgttat tgccgccacg gcatgacagg
    24361 aaccgcgcta atcgcgtttt tcggcgaacc ttctacaact ttgtcggagt aagccagata
    24421 agcgagcgcg ttgcgtttgg cgtcataaaa gcgcacgacc tgtaacgact taaagaccag
    24481 ggacgtgcgt tttttgaata ctacctcgcc ctgagctttg ccgtttttaa tacgatccga
    24541 cagttcaatc ggcccgactt gctgacaaga aatggccgca tcggaggtat cttctgccag
    24601 gcccaatccc cctttaatac caccggtttt cgcccggctc acataacagg tgacattttt
    24661 cacatcggga tcgtcaaagg cttccacaac aattttgtga tccgggccga tcattttaaa
    24721 tacggtgtcg accgaaccaa tctcttctgc atgagccaat ggccccagca ttatcaggct
    24781 taaagacagg atcaaatgct tgtatttcat attgttacca tttttatcta ttacacgagc
    24841 aattagttcg tcacggtaaa taacatattg attaagaaca aaatctcaat tcttttattt
    24901 ccgtcaggcc ctaaaacata ctctactaag aaaaaaacac tgaatgctaa aacagcaaaa
    24961 aatgctatta tccaattacc tgatgtcagg tgctcgttgt tgaaaggatg aggatatttt
    25021 atggatcagg ccggcattat tcgcgacctt ttaatctggc tggaaggtca tctggatcag
    25081 cccctgtcgc tcgacaatgt agcggcgaaa gcaggttatt ccaagtggca cttacagaga
    25141 atgtttaaag atgtcactgg ccatgctatt ggcgcgtata ttcgtgctcg tcgtttgtcg
    25201 aaatcggcgg tcgcactacg cctgactgcg cgtccgattc tggacatcgc gctgcaatac
    25261 cgcttcgact ctcaacagac atttacccgc gcattcaaga agcagtttgc ccagactcct
    25321 gcactttacc gccgttctcc tgaatggagc gcctttggta ttcgcccgcc gctacgtctg
    25381 ggtaaattca caatgccaga gcacaaattt gtcaccctgg aagatacgcc gctgattggt
    25441 gttacccaga gctactcctg ttcgctggag caaatctctg atttccgcca tgaaatgcgt
    25501 tatcagttct ggcacgattt tctcggcaac gcgccgacca ttccgccggt gctctacggc
    25561 ctgaatgaaa cgcgtccgag tcaggataaa gacgacgaac aagaggtatt ctataccacc
    25621 gcgttagccc aggatcaggc agatggctat gtactgacgg ggcatccggt gatgctgcag
    25681 ggcggcgaat atgtgatgtt tacctatgaa ggtctgggaa ccggcgtgca ggagtttatc
    25741 ctgacggtat acggaacgtg catgccaatg ctcaatctga cgcgccgtaa aggtcaggat
    25801 attgagcgat actacccggc agaagacgcc aaagcgggag atcgcccaat taatctgcgc
    25861 tgtgaactgc tgattcctat ccgccgttaa cgctgcagct catctaatgc aggggcgtcc
    25921 agatgcgaga tgtcccctgc ggtttcaacg acccagccag acgccagcca caggctttcc
    25981 tgataatcca cgcgcgaaat agaacagtta cgcagacgta agcggcgctc tgcccatgct
    26041 ggtaatccga gaatcgtact caccagacat cccagtgcaa taccatggct caccagcagc
    26101 ggtcggcttc cctgcggtaa gtcccggcaa gattccagtg ccgcattgac gcgatcgctg
    26161 agctcctgca ttgactcgcc ttcaggaata cgcccgtcaa cggtgccatt gaccagctgc
    26221 cgacgccagt tctcttcttc ttcggtcaga gaatcgatat gtcttttttc cagcacaccc
    26281 atgtttaatt cacgcaggcg agaatcaaag atgatgtcac agccgcaggc ctgggcgatg
    26341 atttccgccg tacgccgggt gcgtcctaaa tcgctactga taatatgcgt aatgccaagc
    26401 tctttggcac gggttgccac ctgcatcgcc tgttgctcac ctttggcggt cagcgggctg
    26461 tcagactggc cctgaatacg tcgctcggcg ttccactgcg tttcaccgtg gcggactagg
    26521 tatacctgta acatgctttt tttccgtata ctgctgtaac gttaattttc tgagtaatgc
    26581 tgattatgca ccaagttgtc tgtgcgacca ccaatcccgc taaaattcag gccattctgc
    26641 aggcatttca cgagatcttc ggcgaaggat cctgccatat tgcatccgtt gccgtcgaga
    26701 gcggtgtacc ggaacaaccc tttggcagtg aggaaacgcg cgctggcgca cgaaatcggg
    26761 tagcaaatgc ccgccgttta cttccagagg ctgatttttg ggtggcgatt gaagctggca
    26821 ttgatggcga cagcactttc agttgggtgg tgattgaaaa cgccagccag cgcggcgagg
    26881 cgcgttcggc aactttgccg ttacctgcgg tgattctgga aaaagttcgt gaaggcgagg
    26941 cgcttggtcc cgtgatgtcg cgttataccg gtattgatga gattggccgt aaggaagggg
    27001 cgatcggcgt gtttaccgcc gggaaactca ctcgcgccag cgtctatcac caggcggtga
    27061 tcctggcgct gagtccgttt cacaatgccg tgtatcaggc attgtaggac ggataaggcg
    27121 ttcacgccgc atccgacatt cggtgcacga tgcctgatgc gacgctgccg cgtcttatca
    27181 ggcctacaaa atcaatcgct tttcagcaac acctcttcca gccactgacg cagctccacg
    27241 ggcgcggctt tcaggctgtt agatccacgc gtaatcgtcg cgatgcccgc gccgagttca
    27301 ttttttaact cacgctgact catttcgcca cgcaacagct cttcgacaat acgcacgcga
    27361 gtccccaacg cttcgcgctc atctggcgtc agcatcaggt ttaacaacgg taaatggaga
    27421 tcgttttggt aggcattctt aagcaggtcg acaaaacgta accactcctg gtgacgctgt
    27481 tctgccatcg ctgctgaata gggtgattgt tgggccataa tatgtcgcca ttgttagcgg
    27541 gggaagcaaa atgactcccc cggttgtact cgctaaagag tacgatagca tatcataaac
    27601 gtgcggatca gtaacgacgt ccccattccg tggcgctcat caacgtcggt ttatccccca
    27661 tgaaatagcg gtagtaagca tcataagcca gcacgttctt cacataaccg cgcgtctcgg
    27721 agaacggaat actctcgaca aatgccacag cgtcgatacg cccggcgctg ttgccaagcc
    27781 aggttcgcac ccgtccaggt ccggcgttat aagctgctga ggagaaaata cggttattgc
    27841 caaactgctg ataaacatat tgcaggtaac tggtgccaat gttgatgttc gtttccggat
    27901 ccagcaattg cccagggctg ctataaccag gaatagagaa catcttcacc gtatgggtcg
    27961 ctgtaccagg cataatctgc atcaggccgc tggcccctac cggtgatttc actttcggat
    28021 tccaggcgct ctcctgacgg gcaatcgcca tcgcatagct ttgcgggatc tctttcccac
    28081 tggtgtaacg tttgaaaaga tcgttgtaag ccagcgggaa tcgctcttcc agatgatccc
    28141 aaagcttccc ggcgatcgtt gcctgaacgc taagatccca ccattggtta ttgaaagcat
    28201 accgagccag ttgagcctgc tctgtttttg acttgctctt caccagattg gcccactcgc
    28261 tacgcgcggt attatcgaga ttccagtaca tcaactcgcg cacgcgcgcc atctccggcc
    28321 cctgagtcag ggtgctgtca acattctgcg gcgctttatc aatcttcagc tcatactctt
    28381 caccgatgcg ttgtgctgca accatcgggt agaaaccacg ctgttgcatg agttgatgca
    28441 aaatctcttt cgcttcagct tcacgtccgc gttccagcaa taaatccgcc tgccagtaac
    28501 gccattcatc tttctctttt gcttccatcg gcagacgcgc cagccaggta ttcaggccgc
    28561 gacgatcgcc ggtgccaagc gccattcgca cacggcgttc aatcaacgac gtggattgcg
    28621 agcgcataat ggcgtcatcg cgccatttcg cctgctcatc ggtgacatca ttgcccatca
    28681 aacgccaggc gacgatatcg cgcagctcct gaatctcatc ttcattaagc tgctgcgcct
    28741 gggcaagcga cgggatcatc agccgtgcat tttcggcatc ctgccgcgcc acactggcaa
    28801 acgccaccgc cgccatttga cgggtaaaat cggtcgcgcc ggttgtacgc gcgaaggtca
    28861 gtaccgtatt agggttgttc gccagtgaaa tgattgccga ggcgatagtc tggtaatcgg
    28921 caggcatctg ccctgccagc actgttacca ggcctgtgtt acccgctttc atcgccagac
    28981 ggatacgctc taaatacgcc agcggatctt gtttacctga cgcacgccag acgctaaata
    29041 acttgtcaca ggcgttaggc tggctcttgc cggttagcca cagctctttc gccccttgcc
    29101 aggcttcttc actctgcccg gtgttccatt tcgcatagta gtaattacat tgcgcttcgg
    29161 tagttccggg cttttccggg ctaaaggcta acaagccacg ccagtcttca cgccgcgcca
    29221 gttcattgac gaaacgagat tgcagcgtgc gagcgggagg aagcgtgggg ttagcgcgaa
    29281 caaagttagt gaccgtcacc gccggttgat tcatcaggtc atcggtgatc tggcggtatt
    29341 ccaggtaggg ataaagcgga taatccttca gtccaggcat catttgttcg accacatcca
    29401 tctgtcgatt atcccaggcc tgcttgattt gcgcgtaacg gctacgctgc tcatccagtg
    29461 agtcggctcg cgccacgctg ctgaccgtca gcagacagac accggcagcc aacagccgcc
    29521 aggtaacttg tttggctttt tccacaagcg catcctctaa gtgtaaatac atcaatgcag
    29581 cgtcataccg cgtaatagat ttatgctaac cagtcattgc cgtttacgcc acgttacgga
    29641 cactttttta cttttactgc gaaggtgatc gaccaactgg gattaggcgg ggaaacaggc
    29701 tacacttcgc ctttgaaaac tcatcatcac gataaacata gaggcgaagt ccaacgtggc
    29761 tcaattcgtt tataccatgc atcgtgtcgg caaagttgtt ccgccgaaac gtcatatttt
    29821 gaaaaacatc tctctgagtt tcttccctgg ggcaaaaatt ggtgtcctgg gtctgaacgg
    29881 cgcgggtaag tccaccctgc tgcgcattat ggcgggcatt gataaagaca tcgaaggtga
    29941 agcgcgtccg cagccagaca tcaagattgg ttatctgccg caggaaccgc agctgaaccc
    30001 ggaacacacc gtgcgtgagt ccattgaaga agcggtttca gaagtggtta acgccctgaa
    30061 acgcctggat gaagtgtatg cgctgtacgc cgatccggat gccgattttg acaagctggc
    30121 cgctgaacaa ggccgtctgg aagagatcat tcaggctcac gacggtcata acctgaacgt
    30181 acagctggag cgtgcggcgg atgcgctgcg tctgccagat tgggacgcga aaatcgctaa
    30241 cctctccggt ggtgagcgtc gtcgcgtggc gttgtgccgc ctgctgctgg aaaaaccaga
    30301 catgctgctg ctcgacgaac cgaccaacca cctggatgcc gaatccgtgg cgtggctgga
    30361 acgcttcctg cacgacttcg aaggcaccgt ggtggcgatt actcacgacc gttacttcct
    30421 cgataacgtt gcaggctgga tcctcgaact ggaccgcggt gaaggtattc cgtgggaagg
    30481 taactactcc tcctggctgg agcagaaaga tcagcgtctg gcgcaggaag cttcacaaga
    30541 agcggcgcgt cgtaagtcga tcgagaaaga gctggagtgg gtacgtcagg gaactaaagg
    30601 ccgccagtcg aaaggtaaag cacgtctggc acgctttgaa gagctgaaca gcaccgaata
    30661 ccagaaacgt aacgaaacca acgaactgtt tattccacct ggaccgcgtc tgggcgataa
    30721 agtgctggaa gtcagcaacc tgcgtaaatc ctacggcgat cgcctgctga ttgatgacct
    30781 gagcttctcg atcccgaaag gggcgatcgt cgggatcatc ggtccgaacg gcgcgggtaa
    30841 atcgaccctg ttccgtatga tctccggtca ggaacagccg gacagcggca ccatcacttt
    30901 gggtgaaacg gtgaaactgg catcggttga tcagttccgt gactcaatgg ataacagcaa
    30961 aaccgtttgg gaagaagttt ccggcgggct ggatattatg aagatcggca acaccgagat
    31021 gccaagccgc gcctacgttg gccgctttaa ctttaaaggg gttgatcagg gtaaacgtgt
    31081 tggcgaactt tccggtggtg agcgcggtcg tctgcatctg gcgaagctgc tgcaggttgg
    31141 cggcaacatg ctgctgctcg acgaaccaac caacgacctg gatatcgaaa ccctgcgcgc
    31201 gctggaaaac gccctgctgg agttcccggg ctgtgcgatg gttatctcgc acgaccgttg
    31261 gttcctcgac cgtatcgcca cgcacatcct ggactaccag gatgaaggta aagttgagtt
    31321 cttcgaaggt aactttactg agtacgaaga gtacaaaaaa cgcacgctgg gcgcagacgc
    31381 gctggagccg aagcgtatca agtacaagcg tattgcgaag taatgcgtaa aatgccggat
    31441 gcggcgcgaa cgccttatcc ggcctacaaa accagcaatt tcaatacgtt gtaaaactgt
    31501 aggcctgata agacgcggta agcgtcgcat caggcattga gcaccgaatg ccggatgcgg
    31561 cgtgaacacc ttttccggtc tacaacagcc ttagcatcct caatttcgtg gcgggtcacc
    31621 cgtgcaggtt ttttcaaacc ttgtttaatc tcgacagcct cttccagaga ggttttcaac
    31681 tcatcaaaaa aactcatttt atacctcgcc tttgagcagt ttcgtcacgg ttatccttgc
    31741 tcccccatca tctcccgcac cagctccacg cagcgcagga aacggctgtc gtaatcgtcc
    31801 tcttcaaccc gcacgaattc gatattgttc tcttccagca tctccaccag caagttctgg
    31861 aactctttgc gatccaccga actgccgagg ctgcgtaaac catccgccac ccacggcgtg
    31921 ttgttctcca gcaggatcac cagatcgaaa cggtattcat caatcagcgc ctgcacgaac
    31981 ggatgctcac gcccttcgta ctttttgcag aacgcctggg tagtgacaaa atcggtgtca
    32041 ataaacgcca ctttattggc gtatttcact gcaaaatcaa tgtattgtgc gtggcccaac
    32101 gcgattttat cgtagtcgga atactgcaat gcgatctcat caccgccgag gtgtgaaaag
    32161 acataatcgc ggccatattc ccacgcactg gtggtgttga agatattggc aagtttgttt
    32221 accagggtgg atttaccgct cgactcgccg ccaaggatcg ccacggtacg cacaaaaaac
    32281 ggcttcactt cggtaggaat atattcccag tagcggaacg ggttttcgcg gatctgcgca
    32341 ccgctgatac tcataaaggt acgtttcgga tcgaccagca ccgtctcgat ccccagatgt
    32401 tccatatact gtggcgcatc ggcttcttcc gaggtgtaga tcaggtccgg ctggatcccc
    32461 ttttcagcca taaacttttt gatgccgttg ctccacacat cccagccatg cggatacggc
    32521 tccatgccct cttcgttgaa agcatgaata cggatatttt tctgatattt aaaggtttgc
    32581 aatagccagc gcagacgatc cggcaccgtg ggctgctgcg acatggcgct gtcttcaaac
    32641 aacgcgcggt cgcgggtatc gtcaaaaccc ataatgatat gcagctcgtc aacctggcta
    32701 caggcgcgct ggataaggta gatatgtccg gtatgcagcg ggtagaactt accgaatacg
    32761 acaccaatgg ttttcttctg ccggggaaat tcaagcccca aaaaacggtg caatgcctcc
    32821 agcttttgcg cgctggggct tttaattttg gcattcagta actggcttaa ataccctttg
    32881 gtcataccgc tggcatcagc tacctgctgt agcgtgcagc cctgttgctt gatggcagtt
    32941 ttcaggtaat caaatgacga catatctccc tccgtatctc tcattataag tcgtcgaaca
    33001 cgctaagcgc gtcggagagt tttttaacgc caaaaatctg catcccttcc ggcgcttttt
    33061 tcggtacgtt agccgccgga acaatcgccc ggcgaaaacc gtgtttcgcc gcttcagaaa
    33121 tccgctcctg gccactgggc accgggcgga tctcccctgc cagcccgact tcaccaaaca
    33181 ccaccagatc ctgtggcagc ggtctgtcac gcaggctgga aaccatcgcc agcagtaacg
    33241 ctaagtcggc gctggtttcg gttaccttca cgccgccgac cacgttcaca aacacatcct
    33301 gatcggccat ttgcagacca ccgtgacggt gcaagacagc cagcaggatt gccagacggt
    33361 tttgttccag ccccactgcc acgcggcgcg gattcgccat tatcgagtga tcgaccagcg
    33421 cctgaatctc caccagcaac ggacgcgttc cttcccacac caccatcacg gagctgccgg
    33481 aggtcacttc atctccgcga cttaagaaaa ttgccgaagg gttgctgact tcacgcagcc
    33541 cctgttcggt catcgcgaag acgcccagct cattcaccgc gccgaagcgg tttttatggc
    33601 tgcgtaaggt gcgaaaacga gagtcggcat cgccatccag aagcaccgaa cagtcgatac
    33661 agtgttccag cactttcggg ccagccagcg aaccatcttt ggttacgtgt ccaaccatga
    33721 caatcgccac accgcgcgtt ttggcgaaac gcgtcagata agccgccgtt tcacgcacct
    33781 gcgccacgct accaggcgat gattgtacat ccgccatatg catcacctgg atcgagtcaa
    33841 ttaccatcag cttcggttgc tcttcttcgg caatcaggca gatctgctcg atgctggttt
    33901 ccgacaacat attgagattg tcagtcggca ggccaaggcg atgagcgcgc attgccacct
    33961 gttgcagcga ctcttcgccg gtgacataca gcgttttcat ctgctgggcc agtttgcaca
    34021 gcgtttgcag caacagcgtg gatttccccg caccagggtt accgccaatc agaatggcac
    34081 ttcctggcac cacgccgccg cctagtacgc ggtcgaactc tttaaatccg gtggaaaaac
    34141 gcggcagctc ttcaaggctg atatcggaga gtttctggac ttttgccacc ccggcgctac
    34201 cggcatagcc gctgagacgc tcgttacgcg ccaccgttgg cgacgcagca agacgcacct
    34261 cggtgatggt gttccaggca tgacaggcac tgcactgccc ctgccagcgc ggataatcgg
    34321 ccccgcattc attacaaaca aaggcgcgtt ttggagcttt tgccacggtg tacctcgtta
    34381 atgctgtgcc gcccgcagga tgacgggcag gcaattactt ctgattcagg ctgccagaga
    34441 ggatgcagaa tacccccatc aggtcagcgt gacggatggt gacttccgcc ttttcattca
    34501 cttttggctt ggcatggtag gcaatcccca gccctgctgc tttgatcatc ggcaggtcat
    34561 tagctccatc gccaatcgcc acggtctgcg ccagcgggat ttcatactcc tgcgcgaggc
    34621 gagtcagagt tttcgctttg tactgtgcgt ctacgatgtc gccgatcaca ttgccggtaa
    34681 atttaccgtc catgatctcc agttcattgg ctaccacggc tgtcaggcgc agcttgtcgc
    34741 gcaggtattc agcaaagaaa gtaaagccgc cggaggcaat cgccactttc cagcccagcg
    34801 tttccagctt gagcaccagc tgcgttaagc ctggcatcag cggcagattt tcacgcacct
    34861 gttgcagaat attggcgtca gcgcctttca gcgtcgccac gcggctgcgc aggctggcgg
    34921 taaaatcgag ttcgccgcgc atcgcccgtt cggttacctc cgctaccatc tcgcccgttc
    34981 cggccagctt ggcaatttca tcaatacatt caatctggat ggcggtggaa tccatgtcca
    35041 tcaccagcaa acccggcgtg cgcaggtgcg ggattttccc cagcggggcg acatccagct
    35101 gcgcttcgtg ggccaggcgt gtagcccgtg cggtgagtga acctgccaga cgaatcacct
    35161 gataatcttc cacgcaccag gcggcaacaa tcaccatcgc cgcacccagt ttgctctggt
    35221 attgggtcag acgctgttta tccagcccac gaccatacag cagccagccg ctacgagctg
    35281 cgtggtaatc cagtggcatc acttcatcac cacttaatga aagaggcaga cccggccata
    35341 aagagacatc ttcaggcagg tcgcaccagg taatgttagg cattaaggct cctgtaaaat
    35401 cgttcgaagc agggaaaata acgcatgagg ctaccttgta tccattgctt ctggcaacat
    35461 taagtctcaa attttcaaag ggtggaagat ggctcgcaca aaactgaaat tccggctaca
    35521 tcgggcagtg attgtcctgt tctgtcttgc cttgttagtg gcactgatgc agggagcgtc
    35581 atggtttagt caaaaccacc agcgacagcg taatccacag ctggaagaac tggcccgcac
    35641 cctggcgcgt caggtgacgc tgaacgttgc accgctgatg cgtaccgact caccggatga
    35701 aaaacgcatt caggcgatcc tcgatcagtt aacggatgaa agccgtatcc tcgacgcggg
    35761 tgtgtatgac gaacaaggcg atcttatcgc acgttctggc gaaagcgtcg aagtgcgcga
    35821 tcggctggcg ctcgacggta aaaaagcagg cggctatttt aaccagcaga ttgtcgagcc
    35881 aattgcgggt aaaaacggac cgctcggcta tctgcgcctg acactcgaca cccatacgct
    35941 cgccaccgaa gcccaacagg tggataacac cactaacatt ttacgcctga tgttgctgct
    36001 ctcactggca atcggcgtag tgctgacccg cacgctgcta cagggtaaac gcacccgctg
    36061 gcagcaatcg cccttcctgt taaccgccag caaaccggtg ccggaagagg aagaaagcga
    36121 gaaaaaagag tgacccatta ctacaagaaa ggaaatcgtt atgtccacat tacgcctgct
    36181 catctctgac tcttacgacc cgtggtttaa cctggcggtg gaagagtgta tttttcgcca
    36241 aatgcccgcc acgcagcgcg ttctgtttct ctggcgcaat gccgacacgg tagtaattgg
    36301 tcgcgcgcag aacccgtgga aagagtgtaa tacccggcgg atggaagaag ataacgtccg
    36361 cctggcgcgg cgcagtagcg gtggcggcgc ggtgttccac gatctcggca atacctgctt
    36421 tacctttatg gctggcaagc cggagtacga taaaactatc tccacgtcga ttgtgctcaa
    36481 tgcgctgaac gcgctcggcg tcagcgccga agcgtccgga cgtaacgatc tggtggtgaa
    36541 aaccgtcgaa ggcgaccgca aagtctcagg ctcggcctat cgcgaaacca aagatcgcgg
    36601 cttccaccac ggcaccttgc tactcaatgc cgacctcagc cgcctggcaa actatctcaa
    36661 tccggataaa aagaaactgg cggcgaaagg cattacttca gtgcgttccc gcgtgaccaa
    36721 cctcaccgag ctgctgccgg ggctcaccca tgagcaggtt tgcgaggcca taaccgaggc
    36781 ctttttcgcc cattatggcg agcacgtgga agcggaaatc atctccccgg acaaaacgcc
    36841 agacttgcca aacttcgccg aaacctttgc ccgtcagagt agctgagagt ggaacttcgg
    36901 tcaggctccg gcattctcgc atctgctgga tgaacgcttt agctggggcg gcgtggaact
    36961 gcatttcgac gttgaaaaag gccatatcac ccgcgcccag gtatttaccg acagcctcaa
    37021 ccccgcgccg ctggaagccc tcgccgggcg actgcaaggc tgcctgtacc gcgcggatat
    37081 gctgcaacaa gagtgcgaag cgctgttggt tgacttcccg gaccaggaaa aagagctacg
    37141 ggagttgtcg acgtggatag cgggggcggt aaggtaactc gctgattaaa atatccaagg
    37201 aaggaaatat gatcgatctg gaaaaccagg aacgggagat tatcaattta atgttcagcc
    37261 agcgtatttc ctggctagcc gccgtgcgta tcaggcataa attatcgctt gctgaagtaa
    37321 gtaaaatgtt aggtatctcg attaattctc taaaacagat cgaaaagacg gaacggctta
    37381 gctcaaacat caaaagcaaa atggcagaaa tttatggttg tccaccagag ttgctgatat
    37441 gcccttcctg gatgacggct gaacataaat gaatagattc gtgaatagat acttgaatgt
    37501 attttatttt ctatccatat gaaaaataat agtaaatcta taaatcagtt gctattgatg
    37561 aatagatact aaaaagccgg agcagtcccc cactccggct taacacgaca atcgcctttc
    37621 agcgaaacac aattactctt tatcgcccag cagaacggat tccagtgcga ttttgatcat
    37681 gtcgttgaag gtggtctgac gctcagcggc agtggtctgc tcgtgagtgc ggatgtggtc
    37741 agacacggtg cagatggtca gggctttcgc gccaaactct gcagccacgc cgtagatacc
    37801 agccgcttcc atttccacgc cgaggatgcc gtatttttcc atcacgtcga acatttcgcc
    37861 gtccggagag tagaacaggt cagcggagaa caggttaccc acgcgagcat caacgcccag
    37921 tgctttagct gcatctactg cgttacgcac catgtcaaag tcagcgatag cggcaaagtc
    37981 atggtcttta aaacggatgc ggttaacttt ggaatcggtg caggccccca taccgataac
    38041 gacgtcgcgc agttttacgt gcggcagaac tgcgccacag gaaccaacgc ggataatttt
    38101 cttcacgccg aaatcggtga tcagttcttt ggtgtagatg gagcaggacg ggatacccat
    38161 accgtgaccc attacggaaa ttttgcggcc tttgtaagta ccagtgaagc ccagcatacc
    38221 gcgaacgttg ttcacttcac gggcatcttc aaggaaagtt tcagcaatat acttcgcacg
    38281 cagcgggtcg cctggcatca aaactacgtc agcgaaatcg cccatttctg cattaatgtg
    38341 tggggtagcc attgttttat cctttttgtg acataacaaa ggcggagtca acgctccgcc
    38401 caaatccatc agaacatggc tttgccatat tccatatcag aagtaccaaa atattttgcc
    38461 agagtctggc cgatatccgc gaaggtttca cggtgaccca gtgagcccgg ttttactttc
    38521 gggccatata ccagtaccgg aatgtgttca cgcgtgtggt cagtaccggt ccaggtcgga
    38581 tcgcaaccgt ggtcagcggt gaggatcagg atgtcgtcat cgcgcagcag agacatcagc
    38641 tccggcagac ggcggtcgaa cagttccaga cccgcagcat aaccggcgac gtcgcgacgg
    38701 tggccccagg aagagtcgaa gtcaacgaag ttggtgaaga cgatggtgtt atcacccgct
    38761 tctttcatct ctttgatggt ggcgtcaaac agcgcgtcca agccagtcgc tttcactttt
    38821 ttggtgatac cgcagttggc gtagatgtct gcaattttac cgacagaaac cacctggccg
    38881 tgtttttcat caaccagttt ctgcagcacg gtcggtgctg gcggctcaac agccaggtcg
    38941 tgacggttac cggtacgctg gaagttacca gctttgtcac cgataaacgg acgagcgata
    39001 acacgaccga tattgtagcc gccgttggtc agctcttcac gggcgatttc gcacagttcg
    39061 tagagtttat ccagaccgaa agtttcttca tggcaggcaa tctggaacac ggagtcagcg
    39121 gaggtataga aaatcggctt gccggttttc atgtgctctt cgcccagttg atccagaatg
    39181 accgtaccgg aagagtggca gttaccgagg taacccggca gattagcgcg ttcgaccagt
    39241 ttatccagca gctcttgcgg gaagctgttt tcgtgatcgg agaaatatcc ccactcaaac
    39301 agaaccggga caccggcgat ttcccagtga ccagacgggg tatctttacc ggatgacatt
    39361 tcgtgcgccc atgcgtacgc gccgataact tcagcgttgc cgtccattcc cgccggaatg
    39421 aaaccggtag aaccttcgtg tgctttcgcc agccccagac ggatcagatt tggcagattg
    39481 agcggccctt tacgaccatt atcagcttcg cctttggcac aagcttctgc gatatgaccc
    39541 agggtgtcag ccccgacgtc accaaagcgt tctgcatctt ctgtagcgcc gatgccgaat
    39601 gagtccagca ccataataaa tgcacgtttc atatgttctc cgtactcagt gcttcaaatt
    39661 taaatgatca gatcagtata ccgttattcg ctgatacggc gatagacagt tggtgtgctt
    39721 tccggtgcgt tatcggcaag tttaattgcc gctttcaccg ctttcgccgc ttcctgccag
    39781 ctgttttcgt ctttcgcgag gataaccgcc agcggacgct gaccgtctac ctggtcgccc
    39841 agacgcgcca tatcagtaaa gccgacgctg taatcgatgg tgtcagatgc ctgacgacga
    39901 ccgccgccca ttgcaaccac tgccatcccc agcgcgcggg tatccatttc actgacaaaa
    39961 ccttcggtat cagcatagac tgctttcgtc agcatcgctg tcggcagata cttcgcgtag
    40021 ttctcaacga agtcggtcgg gcctttttgt gccgctacca tacgaccaaa gacttctgcc
    40081 gctttaccgt tgtccagcac cgcctgcaat ttcgcgcgcg cttcggcgtc atctttcgcc
    40141 agtttgccgg agatcagcat ctccacgcac agcaccatcg tgacatcaaa cagacgcggg
    40201 ttacgatact cacccgtcag gaactgcacc gcttcacgaa cttcaaccgc gttacctgca
    40261 ctggaggcca gtacctgatt catgtcggtg agcagcgcgg tggtgcgcac gccagcgccg
    40321 ttagccacgc caacaatcgc ttcggcaagg gcttcagaga gttcgtaggt cggcataaac
    40381 gcgccgctac ccactttcac gtccatcacc agcgcgtcca gaccttccgc cagtttcttc
    40441 gccaggatcg aggcagtgat cagcgggatg gagtccacgg ttgcggtaat atcacgggtc
    40501 gcgtagaaac gtttatcagc cggagccagt gaactggtct gaccgataat cgccacgccg
    40561 acgtctttaa taatttcgcg gaaacggttg tcatccggga aaatgtcgaa gccagggatg
    40621 gattccagtt tgtcgagcgt accgccagta tgaccgaggc cgcgaccgga gatcatcgga
    40681 atatagccgc cgcaggctgc gaccatcggc cccaacatca gcgaagtgac atcgccgacg
    40741 ccgccggtcg agtgtttatc aacaatcggg ccatccagat gcaggctttt ccagtcgaga
    40801 acggttcctg aatctcgcat cgccatggtc agcgagacac gctcaggcat tgtcatatcg
    40861 tggaagaaaa tggtcatcgc gagggcggca atctgccctt cggaaatagt gttgtcgcga
    40921 ataccgttga taaagaaacg aatttcttca tcgctcagcg catgaccatc acgtttttta
    40981 cgaataattt cttgtgcgag aaacaaggta acctcccgag gaaaagagta tgaggcggct
    41041 tttgccggat gcgtctacgc cttatccggc ctacgggtag atacgtctcg taggcctgat
    41101 aagcgcagcg catcaggcgt aaagcatctt acttagtagc tgctggcgct cttaccgtcg
    41161 ccgtggccca gcgctttcag caggcttgcc agcaggctgg aagcaccaaa gcggtagtga
    41221 cgcgcatcag cccagtcagc accgaacagt tcatctgcga tggcgagata tttctgcgca
    41281 tcttccgcag tacgcacgcc gcccgccggt ttgaaaccaa cggttttttc tacgcccata
    41341 tcacggatca cttccatcat gatgcgagcg ctttccggcg tcgcgttcac agccacttta
    41401 ccggtagagg ttttgatgaa gtccgcaccc gctttgatgg agatttcaga cgctttacgg
    41461 atcagcgctt cgtctttcag ttcgccggtt tcgatgatca ctttcagcag tacattcgct
    41521 gccgcgcaag cctctttaca ggctttcacc aggtcaaaac caacctgctc gtcacccgcc
    41581 atcagcgcgc ggtacgggaa taccacgtca acttcatcag caccgtaggc gattgccgca
    41641 cgggtttctg ccagcgcgat ttcgatgtcg tcgttaccgt gtgggaagtt ggttaccgta
    41701 gcgatacgga tttccggggt gccctgctct ttcagagttt tgcgagcaat cgggataaaa
    41761 cgaggataga tacagatagc ggcggtattg ccgaccggag ttttggcctg atgacacaga
    41821 gcaattactt tctcgtcggt gtcgtcgtca ttcagggtgg tcaggtccat caatttcagt
    41881 gcacgcaggc tgcttgcttt cagatcagtc atttcattct cctggcttgt cgccaataaa
    41941 attcaccttg cgagtttgtt agtattctaa catctactcc gcaacacact tcgatacaca
    42001 tcacaattaa ggaaatctac ttacaagttt gcatcactgt aatgcgatct ggttcaaata
    42061 attcactttc aaatgaatgc gtcagtggtg gcaaacgcat caggatcaaa gtgaacatca
    42121 cgaaacttct tacaatggcg catcaccttc ggcataaaag gaaaagaaga tgcccacttc
    42181 tcatgaaaat gcactgcaac aacgttgcca gcaaattgtc accagcccag tgcttagccc
    42241 ggagcagaag cgccattttc tggcactgga agcagaaaac aatctgcctt acccacagct
    42301 tcctgccgaa gcccgccgcg cgctggatga aggtgtaatc tgcgatatgt ttgaaggtca
    42361 tgcgccgtac aaaccgcgct atgtcttacc cgattacgct cgttttctgg cgaacggttc
    42421 cgaatggctg gagctggaag gcgcgaaaga tcttgatgac gcactctctc tgctgaccat
    42481 tctttaccac cacgtaccgt cggtcacatc gatgccggtc tacctggggc aactggatgc
    42541 gttgttgcaa ccgtatgtta gaattctaac acaaaacgag atcgatgttc gaataaaacg
    42601 tttctggcgt tacctcgaca gaaccctgcc agatgccttt atgcacgcca atatcggccc
    42661 gtctgattcg cccattaccc gcgcgatctt acgtgcagac gcgcaggttt caccgaacct
    42721 gacctttatc tacgatcctg aaatcacccc tgatgacctg ctgctggaag tggcgaagaa
    42781 catctgtgaa tgtagcaaac cgcacatcgc caacggtccg gtgcatgata aaattttcac
    42841 aaaagggggc tacgggattg tgagctgtta caactcactg ccgctggcgg gtggtggcag
    42901 cacgctggta cgccttaacc tgaaagccat tgccgagcgc agcgaatcgc tggatgactt
    42961 ctttacgcgc actctaccgc actactgcca gcagcagatc gccatcatcg atacgcggtg
    43021 tgaattcctc tatcaacaat cacacttctt tgagaatagc ttcctggtga aagaagggct
    43081 gattaaccct gaacgttttg tgccaatgtt tggcatgtat gggctggcgg aagcggttaa
    43141 cttgctgtgt gagaaagaag ggattgccgc gcgctacggt aaagaagccg ccgcaaatga
    43201 agtgggttat cgcatcagcg cgcaactggc ggagtttgtc gccaataccc ccgtgaaata
    43261 tggctggcaa aaacgcgcca tgttacacgc acagtcgggg atcagttccg atatcggcac
    43321 cacgccgggc gcgcgtttgc cgtatggcga tgagccagat ccgatcaccc atctgcaaac
    43381 tgtcgccccg catcatgctt attattattc cggcatcagc gacattctga cgctcgacga
    43441 aaccatcaaa cgtaacccgc aggcactggt acagctttgc ctcggtgcct ttaaagccgg
    43501 aatgcgtgaa tttaccgcca atgtcagcgg taacgatctg gttcgcgtta ccggttatat
    43561 ggtgcgtttg tcggatttag aaaaatatcg cgccgaaggt tcacgcacca acaccacctg
    43621 gctgggcgaa gaagccgcac gcaacactcg tattctggaa cgccagccgc gcgtgataag
    43681 ccatgaacag cagatgcgct ttagtcagta agattatccc cttctcctgc gttgacgggc
    43741 caggcagtcg tctggctctg tttttgcagg gctgcaatct gcgctgcaaa aactgtcaca
    43801 atccgtggac gatgggacgt tgcaatgact gtggggagtg cgtgccacag tgtccgcatc
    43861 aggcgttgca gattgttgac ggcaaagtgg tgtggaacgc tgtggtctgc gagcaatgtg
    43921 atacctgcct gaagatgtgt ccgcaacatg ccacaccaat ggcgcaatcc atgagcgtgg
    43981 acgaagtgct tagccatgtg cgcaaagcgg tgctgtttat tgaagggata acggtaagcg
    44041 gcggcgaagt cacgacccaa ctgccgtttg tagtggcgct gtttactgct atcaaaaacg
    44101 atccgcaact gcgccatctc acctgtctgg tggacagtaa cggtatgttg agcgaaaccg
    44161 gctgggaaaa attactcccg gtgtgtgacg gcgcaatgct cgatctcaaa gcatggggta
    44221 gcgaatgtca tcaacaatta accggacgcg ataatcaaca gattaagcgc agcatctatt
    44281 tgctggcaga gcgcggcaag ctggcggaac tgcgtttgct gatgatccca ggccaggtgg
    44341 attatttgca acacatcgaa gaactggcgg cgtttatcaa gggacttgga gatgttccgg
    44401 ttcgcctgaa cgcgtttcat gcccacggcg tgtatggcga ggcgcaaagc tgggcgagcg
    44461 ccacgccgga agacgttgag ccgttggctg atgcattaaa ggtgcgcggg gtgagccggt
    44521 tgatatttcc ggcgctctat ttgtgacatg ccggatacgg cctacggttc ggcacagact
    44581 tgtaggcctg ataagacgcg tcaagcgtcg catcaggcat tgtgcgccaa ctgccggatg
    44641 cggcgtgaac gccttatccg gcctacggtt cggcacaaac ctgtaggcct gataagacgc
    44701 gttaagcgtc gcatcaggca ttatgcgccg actgccggat gctgcctacg aagggcaaac
    44761 tacccacgaa cgttaaacac cgcatacgtg ttattaagca acacttcggc aatctcatcc
    44821 gcgggttccg ggcgtaactc gcaaagcacg gcaaacacac gggcagcctg ttccgggcgg
    44881 ttaggctgcc cctgaaaacc gttgagcggc atatccggcg catcggtttc cagcaacaac
    44941 gatgccagcg gtaattttgc gatgacatcg cgggttttac tggcgcgtgg ataggtgata
    45001 gtaccgccta cgccgatttt gtagcccagc tgtacaaacc gttcagcctg ttgctggctg
    45061 ccagaaaatc cgtggacaac accagcacgc ggcaaatcgt ggcgtttaag atgcatcgcc
    45121 agtttgtcgt gcgtgcgccg tgaatgcagg atcaccggca gatcgtagcg tttcgccagt
    45181 ttcagttgtt cgtcgagtaa ccactgctgc ctctcaaatt gcggatcgtc gccgaagaga
    45241 tccagaccga tctcccccac cgccaccacc ttcgccggac gcctttccag cgcctgctgt
    45301 agctgctcaa gagacacatc gctatgtttt tccaacatac caggatgcaa gcccaatgcg
    45361 gcatacagcg gttgataatt ttccgctaac gcctgcacgc gggcaaaatt ttccgcctct
    45421 gtcgccggaa caatgatctt gcctacgccc gcttgtgccg cgcgttgcag gctggcctct
    45481 tcatcgccaa taaaaggcgg gaaatcaaaa tggcagtgag tgtcgataaa acggcaaatc
    45541 aagccagatc ctcattgtta aatgtcgggt cgttcgcctg cggagcgtcg ctcacttcgg
    45601 caaccagcgt atcgttggcg actggcgcag gcggaatgac aatcgattcc ggcgtcacca
    45661 ctggcaccag atggcgggta agcggcgctt tttcagttaa tagcttgcca accgtggcga
    45721 ggaaataacg accgcataaa cgcccaagtt tatagtcttc acgcagcgcc ggaatccgac
    45781 tgccaagcgc gatactatgt aatggcttcg gcggataaat ttcggatatc cgcagcttgc
    45841 ccggtggttt ctcaataaat tgctgaatgt cacgatagct ggtttcatga tgctgcacca
    45901 gattgaccag cggctgcagg ctactgtcac ccagccagcg ttccatgcgt ttgaaccact
    45961 gcggcgtgta gtacatttgt gacggcacag tgcggatgac gaccaacgtt ttagcgccct
    46021 gccttgccgc ctctttaacc ggaatcgcat cactgatccc gccatccagg tagttaatgc
    46081 cttccagcga cactccgcta cgataaaagc caggtatcgc actggaggcg cgaatcacat
    46141 ccagccagtt ttgtttggtt ggtaaaaagt aattcggcgc gtagtcatcc tgacgacagg
    46201 cgcacatata aaacgatttg ccgctgtcaa acaaccgcgc ggcggtatcc atttgcaacg
    46261 gcatctggct tgcggtggcc tccaccagcc agtcgagatc gataagattt cctccacgga
    46321 caaagcgcaa tggatcaaaa aattcgcgtt ttgtggtata gcgcatgatg actttgcgcg
    46381 cgtaaccggg ctgattgcag ataaacgccg agaggttctg cgccccggca gatgtgccga
    46441 gataaagatc gaaaggatta aactgcgcgc gcataaactc atccagcacg ccagccgtga
    46501 aaattccacg ctgtccgccg ccttcgcaca ccagagctat tcgtccaggc tggaacggcc
    46561 ttagcgacaa cggcgcaata ttaccaagcg ttacaggtat tctctgcccc acttctgcct
    46621 tcctgttttt gttgtgaaag aaagacaaag taacgcaatt tattattcgc taaaaccgca
    46681 aaagccagtc cgctggactg gctttggata aaattttctt aatggtctga aatctagggt
    46741 cgttttcggc ccatgaacaa actcactagg aacagaataa tcccgacgac aaagacaatt
    46801 ttagctgcgc ctgcagcggt accggccaga ccaccaaacc caagtgcggc ggcgattaac
    46861 gcgataacca gaaatatgat gccccaacga aacataagcg tctcctttac catagttaat
    46921 gtcaccgctt gatatgagcg tgaaaatcac tcaatgcgat atttttagtg tggtgcacat
    46981 cgcgcctccc gacaaagttc gggaggacga attacgacga attacttagt tttcagatca
    47041 tttttaacgc ttttcacacc atctaccgct ttggcgatac tttcagcacg gtcactttgt
    47101 gcctgagaat ctacggtacc ggagagctga accacgccat cggtggtttc aactttcact
    47161 ttacgtgaag ggacgatatc gtccgccagc agtttggctt tgatttcact ggtggtggcg
    47221 gtgtcacccg cgtagtcctt caccgagcct tctttagcgt cgcgaacgtg cagtttgtcg
    47281 ctgacagagg tcacgccttc aacgcctttc gccactttca ctgcctcttc ggcctgggcc
    47341 tgactttcaa cgaaaccgct cagggtcacg actttttgat cggttttgac agagatatcg
    47401 gtgctcttga tgttgtcatg atccaccagc gccgccttca ctttcgcggt gatggcgctg
    47461 tcatccatga aattaccgac tttattcata gagctatcga ctttttgccc tgcgctttca
    47521 ttggtagtct gcgcgttgtt ttccgcgtag gcagagccgg tcgcgacggc agaggtcaac
    47581 attacagcca gcagagtttt cgaaatcttc agtcttgtca tagtcatcga tttattcctg
    47641 tatgtttgct cgtaatttga gcctagcaac acgaggttgc attgctgaat agggagagac
    47701 ttcaccctct acagaagtca atggtcgcca tcacaaaagc gatgggtgat gaataacgac
    47761 cattacagtc tctgaatcag ttattaatat cggtagaatg acaatcgacg gctttagata
    47821 ctgatatcta cgcattgaac ggtatttaac gccatcagaa atgtcatcac tttgttaaat
    47881 atagatcaca attttgaaac cgctcgggat atcagcgaaa acataagcaa aagtgaatgt
    47941 tttaagaaca ttccgtaagc ggctaataag gaagggaaat tgacagggcg cagcggctac
    48001 cgcgccctgg aggcaagaat taatgctcgc gggtctggtg gaactgaacg tccggataac
    48061 gttcctgtgc caggcgcagg ttgaccatgc tggtagcgat gtaagcgagg ttatcgccgc
    48121 catcaagcgc cagttggctt tcgttcttac gcttgaactc ttcgaatttc ttcgcgtctg
    48181 cacattctac ccagcgggca gtggcaacgt tgactgactc atacactgct tcaacgttgt
    48241 attcgctctt caggcgcgct accaccacat caaactgcag cacaccaacc gcaccaacga
    48301 tcagatcgtt gttggagatt ggacggaaca cctgcaccgc gccctcttcg gaaagctgta
    48361 ccagcccttt gagcagctgt ttttgcttca gcggatcttt caggcggata cgacggaaca
    48421 gttctggtgc gaagttcgga ataccggtga acttcatcat ctcaccctgg gtaaaggtgt
    48481 cgccgatctg aatggtgccg tggttgtgca ggccgaggat atcgcccgga tacgcttctt
    48541 caacgtgcga acggtcaccc gccataaagg tcagcgcgtc ggagatcacc acatctttcg
    48601 cagtgcgcac ctggcgcagt ttcatgcctt tttcatattt accggacacc acacgcataa
    48661 acgccacgcg gtcgcggtgt ttcgggtcca tgttggcctg aattttaaat acgaagccgg
    48721 taaatttatc ttcgctcgct tctacggtac gggtatcggt ctgacgcggc atcggcgcag
    48781 gcgcccactc caccaggcca ttcagcatat gatcaacgcc gaagttaccc agcgcagtac
    48841 cgaagaatac cggagtgatt tcgcccgcaa ggaacagctc tttgtcgaac tcgttagacg
    48901 cgcctttaac cagttccagt tcgtcacgca gctgctgtgc cagatcttca ccaaccgcag
    48961 catcgagatc cgggttattc agccctttaa caatgcggac ttcctgaatg gtgtgccctt
    49021 taccgctctg atagagatag gtttcatctt tataaaggtg gtaaacgcct ttaaacagct
    49081 tgccgcagcc aattggccag gtgatcggtg cgcagccaat tttcagctcg ttctcaactt
    49141 catcgagcag ttccatcggg tcgcggatat cacggtcaag tttgttcata aaggtgagga
    49201 tcggcgtgtc gcgcagacgg gtaacttcca tcagcttacg ggtacgatct tcaacacctt
    49261 ttgcggcgtc gataaccatc aggcagcagt ccaccgccgt cagggtacga taggtatctt
    49321 ccgagaagtc ttcgtgcccc ggggtgtcga gcaggttaac caggcaatcg tgatacggaa
    49381 actgcatcac agacgtagta atggagatcc cacgctgctt ttccatctcc atccagtccg
    49441 acttagcgtg ctggttggaa ccacggcctt ttactgtacc ggcggtctga atggcctgtc
    49501 cgaacagcag caccttctcg gtgatggtag ttttaccggc gtccgggtga gaaataatgg
    49561 caaaagtgcg gcgcttcgcc acctcttgca aataaggaga caacgtcata attctttctt
    49621 cttgagtaag cggcatcgcg ccgcgcttgt tgaaaacgaa aaattgcggc tattttaccc
    49681 atcaacgggg gggaggcaat cagtgtttac acaggagctg ctccagttcg tgcaacgaag
    49741 aaacggtcca ggtgggcgcg atgccttctg gttgctcgcg attgtgcgca ttcagccagc
    49801 aggtcgcaag cccggcgttg atgccaccga gaatatcgga ctcggcagtg tcgccaacca
    49861 tcagcacgcg tgaacggtca ggattgcccg cctgttccag cgcataatcg aaaattttct
    49921 tattcggttt ggcaacgcca acttcttcgg aaatcaccag caaatcgaag taatcacgca
    49981 ggcccgtccg ttccagacgc acttgctgca aggcgctaaa accgttggtg atgatgccga
    50041 ttttagcgtt gccacgaatg gcgttaagca gagaaaccgc gcccggcaac ggcgtacaga
    50101 tttccgccat cgcattaata aaggcatcgt taagcaagcc tgcttcaact ttcagccgtt
    50161 cggcccagct ctcgaaacgc ccgtgctgaa gctgtaatga agtgatcgcg ccgttttgat
    50221 aatccaccca cagtggcttg ttaacggcct gatagtcctg aaaatcttca gcggtaaagg
    50281 tgacgctgta atcaagaaac atccgctgca ggccggtgaa tgagtcaaag gtaaacagcg
    50341 tttcatcggc atcaaagaaa atccagtccc acttcattat tccaccttgt cttacatact
    50401 gattggcaac gccatgatga tggcgtcttc gcgaccgtcc gtggtggggt agtaattgcg
    50461 gcgaatcgtc gcctcgttaa agcctaaact ttcgtacagg gcaatggcgg cagcatttga
    50521 ggcacggact tccagccata gtgtcgccac gccgcgtttt tccagttcgt cgatcagatg
    50581 ttccagcagc gccctaccca gcccctgacg ctgatagtcg ggatcgaccg caatattgaa
    50641 caatgtagct tcatccagca ccacttgcgt aatcgcaaac gccgccattt tgccgttttg
    50701 cgttaactga aagttgagat aacgctcgcc ctggttgctg gcaaaggttt tttcactcca
    50761 cggaaaggcg tgggcgcgtt gttcaatgtg gtaagccgct ggtaaatcag tcgtttcgag
    50821 ggaagaaatc gtgttcatat gtgcaaattt gttgccataa cgcggcgcgt gccgttgggt
    50881 ttgcccgtaa ttcggtgagc gccggtgatg ccacctgagc gccttccaat gatagcggtt
    50941 cgtcagtacc caaccgccag ctgttgcagc gactgccttg cggcagcatc gcgatttttt
    51001 ctggcgtcag ttgcagcact tgatcggggc tgacggttaa tgcgcgcaga acatcgctca
    51061 ctaaaggatc agtcagggcg ggaagatcgt ttgccaccat caccagacgg acgtgtgccg
    51121 ggctcgcaat ggcaatctcc ccctgcaacg cgccagggcg acgcagcgac cactgggtaa
    51181 tgcccagttg ctgtaactgc cagtctcgtc gggatgtcat agcgaatcgc tcctgttgtc
    51241 aggggcgcaa atatagcaaa ttcgtcgata ccgcgccaac atatggctat aatcgccgcc
    51301 agtatcaatt gaggagcatt ccatgtctgc atttaccccg gcaagtgaag tcttgctgcg
    51361 tcacagtgat gatttcgaac aaagccgtat tctgtttgcc ggagacttac aggatgacct
    51421 gcccgcgcgt ttagataccg cggccagccg tgctcatacc cagcaattcc accattggca
    51481 ggtgttaagc tgccagatgg gggataacgc ccgttttagt ctggtcgcca cggcgaatga
    51541 tgtcgcagat tgcgatacgc tgatttacta ctggccgaag aacaaaccgg aagcccagtt
    51601 ccagttgatg aatttacttt ctctgctgcc agtggggaca gatatttttg tcgttggcga
    51661 gaaccgcagc ggcgtgcgca gcgctgagca gatgctggca gattatgcgc cgttgaataa
    51721 agtcgacagc gctcgtcgct gtggcctcta ttttggtcgt ctggaaaaac agccagtatt
    51781 tgatgcggat aaattctggg gcgaatacag cgtcgatggc ctgacggtca aaacgctgcc
    51841 tggcgtgttt agccgcgacg gtctggatgt cggtagccag ttgctgctct cgacgttaac
    51901 tccgcacacg aaaggtaaag tgctggatgt cggctgtggc gcgggtgtac tttcagttgc
    51961 ctttgcgcgc cattcgccga aaattcgtct caccttgtgc gatgtctctg ccccggcggt
    52021 agaagccagc cgcgcaacac ttgcagccaa cggtgttgaa ggtgaagtct ttgccagcaa
    52081 cgtcttttcc gaggtgaaag gttgttttga tatgatcatc tccaacccgc cgttccacga
    52141 tgggatgcaa accagcctgg atgcggcgca aacgctgatt cgtggcgcgg tgcgtcatct
    52201 taatagcggc ggcgagctgc gaattgtagc gaacgccttc ctgccttacc cggacgtgct
    52261 ggatgagaca tttggcttcc atgaagttat cacgcaaacc gggcgcttca aggtatatcg
    52321 cgccattatg acccgccagg cgaagaaagg ttgagtatct gatatctgtc gccgcatacc
    52381 gtgtcggatg cggcgtgaac gccttatccg acctacaaaa cctgcacgtt agcccttcgt
    52441 aggccagata agacgcgcca gcgtcgcatc tggcatctgc ccccgcatgc ctaccaaacc
    52501 tgcacgctag tttcctgatg gacatttttc cagcaattac acatcaggcg ataattaact
    52561 attgacgaaa agctgaaaac cactagaatg cgcctccgtg gtagcaattc tttttaagaa
    52621 ttgatggtat gcgaaggtgg cggaattggt agacgcgcta gcttcaggtg ttagtgtcct
    52681 tacggacgtg ggggttcaag tccccccctt cgcaccaaaa accacgttga tattgctcgc
    52741 actgggcgaa ggtggcggaa ttggtagacg cgctagcttc aggtgttagt gttcttacgg
    52801 acgtgggggt tcaagtcccc cccctcgcac caaacgaggc gatatcaaaa aaagtaagat
    52861 gact
//
LOCUS       DADMGC010000013        52645 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.013, whole genome shotgun sequence.
ACCESSION   DADMGC010000013 DADMGC010000000
VERSION     DADMGC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.013"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..320
                     /locus_tag="JNZ73_000774"
     CDS             <1..320
                     /locus_tag="JNZ73_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8731160.1"
                     /translation="VGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDS
                     NSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQV
                     TYP"
     gene            433..1227
                     /gene="panB"
                     /locus_tag="JNZ73_000775"
     CDS             433..1227
                     /gene="panB"
                     /locus_tag="JNZ73_000775"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAY8731161.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1239..2090
                     /gene="panC"
                     /locus_tag="JNZ73_000776"
     CDS             1239..2090
                     /gene="panC"
                     /locus_tag="JNZ73_000776"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAY8731162.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSTLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2164..3066)
                     /locus_tag="JNZ73_000777"
     CDS             complement(2164..3066)
                     /locus_tag="JNZ73_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY8731163.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIPQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3340..3720
                     /gene="panD"
                     /locus_tag="JNZ73_000778"
     CDS             3340..3720
                     /gene="panD"
                     /locus_tag="JNZ73_000778"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAY8731164.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3724..4953)
                     /locus_tag="JNZ73_000779"
     CDS             complement(3724..4953)
                     /locus_tag="JNZ73_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY8731165.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5017..5457)
                     /locus_tag="JNZ73_000780"
     CDS             complement(5017..5457)
                     /locus_tag="JNZ73_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY8731166.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5562..6332)
                     /locus_tag="JNZ73_000781"
     CDS             complement(5562..6332)
                     /locus_tag="JNZ73_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8731167.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6329..7255)
                     /locus_tag="JNZ73_000782"
     CDS             complement(6329..7255)
                     /locus_tag="JNZ73_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8731168.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCLNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7364..8026
                     /gene="can"
                     /locus_tag="JNZ73_000783"
     CDS             7364..8026
                     /gene="can"
                     /locus_tag="JNZ73_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAY8731169.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="JNZ73_000784"
     CDS             complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="JNZ73_000784"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAY8731170.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8809..11199
                     /locus_tag="JNZ73_000785"
     CDS             8809..11199
                     /locus_tag="JNZ73_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HAY8731171.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHAFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11246..12795)
                     /gene="cueO"
                     /locus_tag="JNZ73_000786"
                     /pseudo
     CDS             complement(11246..12795)
                     /gene="cueO"
                     /locus_tag="JNZ73_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
     gene            12961..13308
                     /locus_tag="JNZ73_000787"
     CDS             12961..13308
                     /locus_tag="JNZ73_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HAY8731172.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13414..14280
                     /gene="speE"
                     /locus_tag="JNZ73_000788"
     CDS             13414..14280
                     /gene="speE"
                     /locus_tag="JNZ73_000788"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAY8731173.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14296..15090
                     /gene="speD"
                     /locus_tag="JNZ73_000789"
     CDS             14296..15090
                     /gene="speD"
                     /locus_tag="JNZ73_000789"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAY8731174.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15128..15490)
                     /locus_tag="JNZ73_000790"
     CDS             complement(15128..15490)
                     /locus_tag="JNZ73_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HAY8731175.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="JNZ73_000791"
     CDS             complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="JNZ73_000791"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HAY8731176.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18618..20471
                     /locus_tag="JNZ73_000792"
     CDS             18618..20471
                     /locus_tag="JNZ73_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HAY8731177.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVIPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNSITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQATASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSTVRERLSERGARRLER"
     gene            complement(20542..21972)
                     /gene="lpdA"
                     /locus_tag="JNZ73_000793"
     CDS             complement(20542..21972)
                     /gene="lpdA"
                     /locus_tag="JNZ73_000793"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAY8731178.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPNPKAKKK"
     gene            complement(22297..24189)
                     /gene="aceF"
                     /locus_tag="JNZ73_000794"
     CDS             complement(22297..24189)
                     /gene="aceF"
                     /locus_tag="JNZ73_000794"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAY8731179.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPVAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24204..26867)
                     /gene="aceE"
                     /locus_tag="JNZ73_000795"
     CDS             complement(24204..26867)
                     /gene="aceE"
                     /locus_tag="JNZ73_000795"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HAY8731180.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27028..27792)
                     /gene="pdhR"
                     /locus_tag="JNZ73_000796"
     CDS             complement(27028..27792)
                     /gene="pdhR"
                     /locus_tag="JNZ73_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HAY8731181.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28333..29706
                     /gene="aroP"
                     /locus_tag="JNZ73_000797"
     CDS             28333..29706
                     /gene="aroP"
                     /locus_tag="JNZ73_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAY8731182.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29749..30603)
                     /gene="ampE"
                     /locus_tag="JNZ73_000798"
     CDS             complement(29749..30603)
                     /gene="ampE"
                     /locus_tag="JNZ73_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HAY8731183.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30600..31151)
                     /gene="ampD"
                     /locus_tag="JNZ73_000799"
     CDS             complement(30600..31151)
                     /gene="ampD"
                     /locus_tag="JNZ73_000799"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HAY8731184.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31239..32132
                     /gene="nadC"
                     /locus_tag="JNZ73_000800"
     CDS             31239..32132
                     /gene="nadC"
                     /locus_tag="JNZ73_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAY8731185.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNDKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32335..32775
                     /gene="ppdD"
                     /locus_tag="JNZ73_000801"
     CDS             32335..32775
                     /gene="ppdD"
                     /locus_tag="JNZ73_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HAY8731186.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32785..34170
                     /gene="gspE"
                     /locus_tag="JNZ73_000802"
     CDS             32785..34170
                     /gene="gspE"
                     /locus_tag="JNZ73_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HAY8731187.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNTVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAVQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPAIRQLISANTEVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGK"
     gene            34160..35362
                     /gene="hofC"
                     /locus_tag="JNZ73_000803"
     CDS             34160..35362
                     /gene="hofC"
                     /locus_tag="JNZ73_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HAY8731188.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35397..36440)
                     /gene="guaC"
                     /locus_tag="JNZ73_000804"
     CDS             complement(35397..36440)
                     /gene="guaC"
                     /locus_tag="JNZ73_000804"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAY8731189.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            36665..37285
                     /gene="coaE"
                     /locus_tag="JNZ73_000805"
     CDS             36665..37285
                     /gene="coaE"
                     /locus_tag="JNZ73_000805"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAY8731190.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLIENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37285..38028
                     /gene="zapD"
                     /locus_tag="JNZ73_000806"
     CDS             37285..38028
                     /gene="zapD"
                     /locus_tag="JNZ73_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HAY8731191.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38038..38235
                     /gene="yacG"
                     /locus_tag="JNZ73_000807"
     CDS             38038..38235
                     /gene="yacG"
                     /locus_tag="JNZ73_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HAY8731192.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38335..38724)
                     /gene="mutT"
                     /locus_tag="JNZ73_000808"
     CDS             complement(38335..38724)
                     /gene="mutT"
                     /locus_tag="JNZ73_000808"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HAY8731193.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38784..41489)
                     /gene="secA"
                     /locus_tag="JNZ73_000809"
     CDS             complement(38784..41489)
                     /gene="secA"
                     /locus_tag="JNZ73_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAY8731194.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKLGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41551..42063)
                     /gene="secM"
                     /locus_tag="JNZ73_000810"
     CDS             complement(41551..42063)
                     /gene="secM"
                     /locus_tag="JNZ73_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HAY8731195.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42294..43211)
                     /gene="lpxC"
                     /locus_tag="JNZ73_000811"
     CDS             complement(42294..43211)
                     /gene="lpxC"
                     /locus_tag="JNZ73_000811"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HAY8731196.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43312..44463)
                     /gene="ftsZ"
                     /locus_tag="JNZ73_000812"
     CDS             complement(43312..44463)
                     /gene="ftsZ"
                     /locus_tag="JNZ73_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAY8731197.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44524..45786)
                     /gene="ftsA"
                     /locus_tag="JNZ73_000813"
     CDS             complement(44524..45786)
                     /gene="ftsA"
                     /locus_tag="JNZ73_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAY8731198.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45783..46613)
                     /gene="ftsQ"
                     /locus_tag="JNZ73_000814"
     CDS             complement(45783..46613)
                     /gene="ftsQ"
                     /locus_tag="JNZ73_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HAY8731199.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46615..47535)
                     /locus_tag="JNZ73_000815"
     CDS             complement(46615..47535)
                     /locus_tag="JNZ73_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAY8731200.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLKASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47528..49003)
                     /gene="murC"
                     /locus_tag="JNZ73_000816"
     CDS             complement(47528..49003)
                     /gene="murC"
                     /locus_tag="JNZ73_000816"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAY8731201.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49057..50124)
                     /gene="murG"
                     /locus_tag="JNZ73_000817"
     CDS             complement(49057..50124)
                     /gene="murG"
                     /locus_tag="JNZ73_000817"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAY8731202.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNGWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50121..51365)
                     /gene="ftsW"
                     /locus_tag="JNZ73_000818"
     CDS             complement(50121..51365)
                     /gene="ftsW"
                     /locus_tag="JNZ73_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HAY8731203.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51365..>52645)
                     /gene="murD"
                     /locus_tag="JNZ73_000819"
     CDS             complement(51365..>52645)
                     /gene="murD"
                     /locus_tag="JNZ73_000819"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAY8731204.1"
                     /translation="GLGLTGLSCVDFFLARGVTPRVMDTRMTPPSLDKLPEAVERHTG
                     SLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCREAQAPIVAITGSN
                     GKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELSSFQLETTSSLQA
                     VAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALTMPIRGADERCVS
                     FGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAALALADAAGLPRA
                     SSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGLHVDGTLHLLLGG
                     DGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETMEQAMRLLAPRVQP
                     GDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
BASE COUNT        12637 a        14495 c        12929 g        12584 t
ORIGIN      
        1 aggttggtac tgaaaacggg caactccttg gtaatactct cacaggtaat gacgcagcaa
       61 aaggagtcgg tgtactcata gaaggtttag caactagtaa aaatcctcta atgacattga
      121 aacctaatga ttcaaattct gtttataaag attacgaccc gagaggcaaa gacgatacaa
      181 caggaggagt ttacccggat caagatacag gtataacata ccctctccat ttccaggcca
      241 cgctacaaca ggatggaact ataccaatag aagctggtga gtttaaagcc accagtactt
      301 tccaggtaac ctacccttaa taagtccacc gcaccgccat catctggcgg tgcggtaatt
      361 gataaatcat tgcccccacc aatgtgactg ataaaatgcc aggcaacacg acatcattta
      421 tcaggatacg ttatgaaacc gaccaccatt gcttcacttc agaaatataa gcaggataaa
      481 aagcgtttcg cgaccatcac cgcttacgac tacagcttcg cgaaactatt tgctgatgaa
      541 gggcttaacg tcatgctggt aggcgactcg ctgggcatga ccgttcaggg ccacgattcc
      601 actctgccag taaccgttgc tgatatcgcc taccacactg ccgccgtacg tcgcggcgca
      661 ccaaactgcc tgctattggc tgacctgccg tttatggcgt atgccacgcc ggaacaagcc
      721 tttgaaaacg ccgcaacggt tatgcgtgcc ggtgccaata tggtcaaaat tgagggcggt
      781 gagtggctgg tcgaaaccgt aaaaatgctg accgaacgtg ccgttcctgt atgtggtcac
      841 ttaggtttaa ccccacagtc agtgaatatt ttcggtggct acaaagtaca ggggcgcggc
      901 aatgaagcga gcgatcgact actcagcgat gcattagcct tagaagccgc gggggcacag
      961 ctgctggtgc tggaatgcgt gccggttgag ctggcaaaac gtattaccga agcactggcg
     1021 atcccggtta ttggtattgg tgcaggcaac gtcactgacg ggcaaatcct cgtgatgcac
     1081 gacgcctttg gtattaccgg cggtcacatt cctaaatttg ctaaaaattt cctcgccgaa
     1141 acgggcgaca tccgcgcggc tgtgcggcag tatatggctg aagtggagtc cggcgtttat
     1201 ccgggcgaag aacacagttt ccattaagga gtcacgttgt gttaattatc gaaaccctgc
     1261 cgctgctgcg tcagcaaatt cgccgcctgc gtatggaagg caagcgcgtg gcgctggtgc
     1321 ctactatggg taatctgcac aatggccata tgaagctggt cgacgaagcc aaagcccgcg
     1381 ccgatgtggt cgtcgtcagt attttcgtta acccgatgca gttcgaccgc ccggaagatc
     1441 tggcacgtta tccacggacc ttgcaggagg actgcgagaa gctaaacaaa cgtaaagtgg
     1501 atttagtttt cgccccttcg gtaaaagaga tctacccgaa cggtactgaa acccacactt
     1561 acgttgacgt tcctggcctt tcaaccatgc tggaaggtgc cagccgtccg ggacattttc
     1621 gcggcgtttc gactattgtc agcacgctgt tcaacctggt ccagccggac atcgcctgct
     1681 tcggtgaaaa agattttcag caactggcgt tgatccgcaa aatggttgcc gatatgggct
     1741 tcgatattga gattgtcggt gtgccaatta tgcgcgccaa agacggtctg gcgctaagtt
     1801 cccgtaacgg ttatctgacg gcggaacaac gcaaaattgc gcctggtctg tacaaagttt
     1861 taagttcgat tgctgacaaa ttgcaggctg gggaacggga tctcgatgaa attattacca
     1921 ttgcggggca agaactgaat gaaaaaggct tccgcgccga tgatattcag attcgcgatg
     1981 ccgacacatt gctggaagtt tctgaaacca gcaaacgggc agtaattctg gtagccgcct
     2041 ggcttggcga tgctcgcctg atcgacaaca aaatggtcga gctggcgtaa tacttaactg
     2101 gcgctacggc tgatggcgcc agttattaat ttaccccacg tcatctgtca taacacaggt
     2161 aacttaaata aggttaatta ccttatcaat ctcagcaaga ggtaaatttg ccatctctgc
     2221 aacgtcttcc cgagacattc ctttacttag aagacgcagg gcgaattctt gcctttcctc
     2281 ctgtcttccc tgcggaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2341 catcatagac ttccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2401 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2461 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2521 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2581 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gcgccagctc
     2641 cggcgagtaa aacatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     2701 ataaaacagt atcggcacca ccagcggcag cttatcgtgg ccagcctcca gatgccggtg
     2761 catggcagca atagaataac gcatcatgcg aaaggccatt ttcttatccg gcttgctttg
     2821 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     2881 gtccgtgctg tgacctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     2941 gttgagatca caaagttcgc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3001 cgtctccgca tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc
     3061 atccatcttc cctgtcttcc gtgtggcctt gcgtgaaggc agtatccagt caaggcgtgc
     3121 tatcgtcagc cacctcttta ttgtttaccg agcagcattc ataaaaatat caattcgtta
     3181 caggcgttac atggcacgct tcggcgcggt acgcaaactg ctgacgcaaa tgccatcaga
     3241 tgagtagaca ctcatcaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcac
     3301 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3361 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3421 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat cgatatctgg
     3481 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3541 attatttctg ttaacggtgc ggcggcccac tgcgccagcg tcggcgatat tgtcatcatc
     3601 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa tgtcgcctat
     3661 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     3721 ttgttatccc tgcggctggt tactcaccag ccgcgacatc ttctccagcg aatccgttct
     3781 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     3841 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     3901 gccgccaaac ggatacgaaa gataccagac atgtggatta aattgcgcca gagcgcggcg
     3961 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4021 ataaccatct acccgatgca aaaaatgggt atgtgactgg aaatcaaaaa catcgcgaat
     4081 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4141 acgtttgatg cgtgaggtaa caataaacac cgtcgccttc atgccatatt gtttcaacac
     4201 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4261 cgcagggaga ttgatcgtat tcttcacgta accttccagc tgagccatgc tcagtgtcgc
     4321 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4381 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4441 cggcaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4501 acggatctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4561 caaattgtcc gccagtaccc caaatggcgc actgcccaca ctcggcgcgt tgtagaccgg
     4621 cgtatctttc caggtaatta agttctgatt actcagcggc ttgttaaggt cacccaaacc
     4681 gtcttcaact ttttgtcgcc cctgaactgg ctcgagatgc cctttatcga taaacccttt
     4741 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctctacgg caatgatttg
     4801 tccggcccga atattcccca cggtcaccat cttgtcacca atttgcgccc agaccgcagc
     4861 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     4921 catcagcagg agaataacag cttgtttata catgaaaaaa taaccgaggg tgaaacaacg
     4981 cttttattgt agcaaaagcg ccatcagaat cgcgggttaa tactcataca aattatgttt
     5041 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5101 cagttcgaca atacactcac gaaactctga actggtcatg gtttcacggc aagccatcat
     5161 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5221 taataatgaa gccacgcgat acggtggcgc tccggctata tcggttaaga agatgacacc
     5281 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5341 taatccgcgc cagaaattca cggcccggca ctgaagaagt gccccatgtt ttttctccag
     5401 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5461 cttcctcctt agccgaaaat cagcctaccc tgaccattgt tgataaaggg tgatagccgt
     5521 caaaactcct ctcccccgaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5581 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     5641 ccaaaggtag tgaccagcgg cacatcattt ataccgagga agccgtagcg gaaaccactg
     5701 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     5761 agtgagtaaa agaccccgcc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     5821 ctgatgtcat cgaacgtttt ggcaaacacg ccgttcagca aacccgcaag ggagaatagc
     5881 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     5941 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6001 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6061 ttacgctgga acttggcacc aaaaaatgat gacgcgacgt tagcgtaggc gttggtgatc
     6121 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6181 ccaatacgcg aacctatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6241 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6301 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6361 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6421 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgtagc acttcaactt
     6481 ccagcgtcgc agtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6541 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6601 aggtattttc caccagctca ccatgttgaa taatgccgat attgagacac agcatttctg
     6661 cttcttccag gtagtgtgtg gtaagaatga tggtggtgcc tttgtcgttt aaatccttca
     6721 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     6781 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     6841 cagataacat acgcgcacgt tcgttgcgtt ttccccataa atcgagctgc ttaagatatt
     6901 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     6961 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7021 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7081 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7141 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7201 gaacgccgcc tggatagttt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7261 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7321 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7381 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7441 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7501 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7561 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7621 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     7681 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     7741 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     7801 gtgtgaactg aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc
     7861 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     7921 cttgctgcgt gatctggatg ttaccgccac caaccgcgaa acccttgagc aacgttaccg
     7981 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8041 atgcaacaca tccggcaact tcacacttac tcgtccagca gaatcacttt gccaatatac
     8101 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8161 atcgagaaac cgataaattc aaccgggacg ttcacttcac gacgggacgg tttatccagc
     8221 agcgtacaaa tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8281 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8341 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8401 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8461 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8521 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8581 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     8641 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     8701 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaatct
     8761 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     8821 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     8881 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     8941 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg
     9001 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9061 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9121 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9181 gtggtcgcac tgctgattag cggtggtatc ctgacctggg ccggatttaa cgatccgcag
     9241 gagatcaacg gcaccttaag cgccgatgcc acacctgccg aagctatctc ccccgttgcc
     9301 gatcaggact ggcctgcgta tggccgcaat caggaaggtc aacgcttttc accactgaag
     9361 caaattcacg ccgataacgt ccacaagctg aaagaagcct gggtgttccg tactggcgat
     9421 gtgaagcagc cgaacgatcc gggtgaaatc accaatgaag tgacgccgat taaagtgggc
     9481 gatacccttt acctgtgtac cgctcaccag cgcctgtttg cgcttgatgc cgccagcggc
     9541 aaagagaaat ggcattacga tcctgagctg aaaaccaacg agtctttcca gcacgtaacc
     9601 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
     9661 gattgcccgc gtcgtatcat tcttccagtg aatgatggtc gcctgattgc gattaacgct
     9721 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
     9781 atgccagaca ccaaaccggg tctgtatgag ccgacttctc cgccaattat caccgataaa
     9841 accatcgtga tggccggttc agtcaccgat aacttctcaa cccgcgaaac gtctggcgtg
     9901 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc gggcgcgaaa
     9961 gatccgaacg caatcccgtc tgacgaacac gcctttacct ttaactcacc aaactcgtgg
    10021 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10081 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10141 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10201 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10261 gtgccgatta tttacgctcc ggcgaaaacc ggtaacattt ttgtgctcga tcgtcgtaat
    10321 ggcgaactgg tggttccggc cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat
    10381 tacgttaccc ctactcaacc attctcggaa ctgagcttcc gtccgacgaa agatttgagc
    10441 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10501 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggtacgct ggtcttcccg
    10561 ggtaacctgg ggatgttcga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10621 attgccaacc caatggcact gccgtttgtt tcgaaactgc tcccgcgtgg tcctggcaac
    10681 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    10741 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccatttgg tctgccatgt
    10801 aaacagccag catggggtta tatctcggcg ctggatctga aaactaatga agtggtgtgg
    10861 aagaaacgta ttggtacgcc gcaggacagt atgccgttcc cgatgccggt tccggtgccg
    10921 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    10981 atcgccgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11041 tggcagggtc gtttaccggc gggtggtcag gcaacaccga tgacctatga agtgaatggc
    11101 aagcagtacg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11161 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagaaatcc
    11221 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11281 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11341 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11401 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcgtg ccgtgaatat
    11461 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11521 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11581 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    11641 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    11701 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    11761 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    11821 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggtgcaatcg
    11881 ccatccccat ctggctgacc ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct
    11941 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12001 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12061 aattgagcga gcgggcatta cagccattga gcaaacgcag gcgcagccaa ccacgcgggg
    12121 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12181 cggcggtcat cacatccagt tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct
    12241 gaacgatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12301 cgtcatcttc aatcaccacc agaccagcca gccccatcgc cacctgtcgc cggtcttgcc
    12361 atgttgatgt ggatggaacc agcaggtagc ggcaggttga tcaacgttca acgtcaccga
    12421 gcgcttgcca cctggcggaa taattccctg tgggccgccg tcgacgtcac ccggtacttc
    12481 cagcccgtgc cagtgcaacg tcgtctcttc cgccagttgg ttgtagatat caaccgttac
    12541 cgctttgccg cgctgtaatt tcaccgccgg ccccagcaga ttgccgttat agccccaggt
    12601 agttgcagtt ttctcgccaa aggtggactg acctgcgcca atagttaact gaatgcgatt
    12661 acgggcatcg gtcgtgagca aatcagggat cggtaacgtt gggcgttccg ccgcaaagac
    12721 tgcacggctc cacagtggca aggctgaagc cacacccagt gcgacggaat atttcaagaa
    12781 atcacgacgt tgcatagtta tttccttatt cttaagcagg caaaacaaaa tcaaacgttg
    12841 agcatagtcc ttccccttac gggaaggtca agccgcagac acaatttaat cgacaataat
    12901 ggtcgtcgcc tcgctcgcag tgtgctaacg tttatcttct ttaagcccct ggtagaagca
    12961 atgaagacgt ttttcagaac agtgttattc ggcagcctga tcgccgtttg cgcaaacagt
    13021 tacgcgctca gcgagtctga agccgaagat atggccgatt taacggcagt ttttgtcttt
    13081 ctgaagaacg attgtggtta ccagaactta cctaacgggc aaattcgtcg cgcactggtc
    13141 tttttcgccc agcaaaacca gtgggatctc agtaattacg acaccttcga catgaaagct
    13201 ctcggtgaag acagctaccg cgatctcagc ggcattggca ttcccgtcgc taaaaaatgc
    13261 aaagccctgg cccgcgattc cttaagcctg cttgcctacg tcaaataatc cctgatacct
    13321 ttttgtagaa atattgaccg tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt
    13381 ttttacgggt gttaacaaag gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa
    13441 acgctacacg accagtttgg gcagtacttt gcggtagata acgttctgta tcatgaaaag
    13501 accgatcacc aggatctgat catttttgag aacgctgcat ttggtcgcgt aatggcgctg
    13561 gatggcgtag tacaaaccac cgagcgcgac gagtttatct atcatgagat gatgacccat
    13621 gttccgctac tggcccacgg tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt
    13681 gccatgctgc gtgaagtaac ccgacataaa aacgttgagt caatcacgat ggtggaaatc
    13741 gatgcgggtg tcgtgtcgtt ctgccgtcag tatctgccca accataacgc cggtagctac
    13801 gacgatccgc gctttcaact agtgatcgac gatggcgtca atttcgttaa tcaaaccagc
    13861 cagacctttg atgtcattat ctccgactgc accgatccta tcggtcccgg cgaaagcctg
    13921 ttcacctcag cattttatga aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc
    13981 gcacaaaacg gcgtctgctt tttacagcag gaagaagcca tcgacagcca tcgcaaactc
    14041 agccattact tcagcgacgt tggcttttat caggcggcga tcccgaccta ttacggcggt
    14101 atcatgactt ttgcatgggc gacagataac gacgccttac gccatctctc aaccgaaatt
    14161 attcaggcgc gttttctcgc ttctggcctg aaatgccgtt attacaatcc ggcaatccat
    14221 acggcagctt ttgccttacc tcagtatctg caagacgcac tggcttcaca gccgtcctaa
    14281 ggagaagata agaaattgaa aaaactgaaa ctgcatggct ttaataatct gaccaaaagt
    14341 ctgagttttt gtatttacga tatctgctac gccaaaactg ccgaagagcg cgacggttat
    14401 attgcttata tcgatgaact ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc
    14461 tgttccatta tcggggccaa tattcttaac atcgcccgcc aggattacga accacagggt
    14521 gccagcgtca ctattctggt gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca
    14581 gaacaccccg gcccactgcc agaaacggtg gttgctcatc tcgataaaag tcatatttgc
    14641 gtacatacct acccggaaag tcatcctgaa ggcggtttat gtaccttccg cgccgatatt
    14701 gaagtctcta cctgcggcgt gatttctccg ctgaaggcgc tgaattacct gatccaccag
    14761 cttgagtccg atatcgtaac cattgattat cgcgtgcgcg gttttactcg cgacattaac
    14821 ggtatgaagc actttatcga ccatgagatt aattcgattc agaactttat gtctgacgat
    14881 atgaaggcgc tgtatgacat ggtggatgta aacgtctatc aggaaaatat cttccatacc
    14941 aagatgttgc ttaaagagtt cgaccttaag cactacatgt tccacaccaa accggaagat
    15001 ttaaccgaca gcgagcgcca ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt
    15061 tattacgggc gcaatatgcc agctgtttaa cggctctggc ggagctccca ggctccgcca
    15121 gatttattta cttctgctgc acgaaattgc ggtaagccgc cacgacctgc agaaaatcct
    15181 caacgccgca tagcgacagg ctttcttcgt cgtagtagtt catcccctct tccatttcat
    15241 cgccagcgaa ttccagttga ttggcgcgaa ccatcacctc ttcagcgtcc atccacaggg
    15301 tgtattcatg ccctgcccgt tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt
    15361 gttccacttc atcaagcaag gccaggtttt ctttcacctc ttcattaaac cagtgcccga
    15421 ccacttcatg ccccatggac atacgcacct ttaccactcc ggtaatatcg cgcagaaatt
    15481 cgtaatccat agtgtgttcc tctgctcccg gcaatgcgcc gaaatcgcat cgcgccattg
    15541 atgtaattat cgcagcctta ccggagaaaa acagcggaac ggcgatggtt tagaaataaa
    15601 aaatgcccgg gaatctcatt caccgggcat tggtgtcgtt tatgcgcagc gcgtgcgctt
    15661 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    15721 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    15781 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    15841 gcagaagcca ggaagacatt cgcgccagta cccaaacggt tcgggaagtt acgggtagag
    15901 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagggaacag
    15961 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttccgtc
    16021 agttgcgcgg cgtccatacg ggttggcggt gccacccaca ggcgggtcgg caactgacct
    16081 ttgtgcgcat ccagcagttt accagcggca cggaagtgac cgatgttagt catgcaagaa
    16141 ccgataaata cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcatcc
    16201 ggatcgttcg gagcacacag gattggctct ttaatctccg ccagatcgat gtcgatcact
    16261 gccgcgtatt ccgcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16321 atgccctgaa tacggcgttc caaggtacga cgatcgccgt aaccttccgc gatcatccac
    16381 ttcagcagga cgatgttgga gctcaggtat tcgatgatcg gttctttgtt cagcttgata
    16441 gtacaaccag cggcagaacg ctcggcggac gcatcggtta gctcaaaggc ctgctcaact
    16501 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16561 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcggaatggc gtgtaccaga
    16621 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    16681 atatccagcg gcattacgcc agttgcggca gcaaacgcca ccagaccaga acccgccggg
    16741 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    16801 agcagcatac ggttcagcca ggagtgaatg acgccgtcac ccggacgcag cgacacaccg
    16861 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    16921 tacgccgcgg tgtggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    16981 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc
    17041 attttcggtt cgcagtacgc gcccggacga atgcctttca cgccacaggc acggcctacc
    17101 attttctgcg ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17161 acatcactgt gcggcagacc aagtgcttca cgagctttgg tggtcaggcc acgcccgata
    17221 atcagcggaa tacggccgcc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17281 aaggtcgcca gcagttcgcc tgtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17341 tcaatcacgt cgcccatgtt caggttagag acgttgactt cgattggcag tgcacccgcg
    17401 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17461 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17521 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17581 tgttgcagag cttcaatctg tttgatcggg ccaacaacac ctggctggtc tggctcaata
    17641 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac
    17701 gcatccggtg ccggagaaag gtcatcggtg ttggtttcgc cagtgacttt aaagacggta
    17761 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    17821 tgcattacct gcttcgcata ttcgttgcct gctttcgctt tctcttctac gtcatagaag
    17881 ttatcgaaca tcagcagcgt gtgagaaagt gctttggcag caataggtgc cagtttggca
    17941 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt gcccagcagt
    18001 tcgatagctt tttccggagt cagcagaggg gatttagctt cgcctttagc gatagcagcc
    18061 aggaagcctg cttttacata ggcggcttca tcgacgcctg ggggaacacg gttggttaac
    18121 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagcgcg
    18181 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18241 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18301 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18361 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18421 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18481 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18541 cgcctcttta tactcggatt cacagcacct gcgggtggca gttcgccgac cattgcgatt
    18601 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcgctaa
    18661 tttgcagtgc cgggctttgt gctgcctctg ccgggctata tataaaaagc cgcacagtgg
    18721 aagcgcctgt agaaccgcaa tcgacacaac tggctgtgtc tgacgctgcc gctgttacgc
    18781 ttcctgcaac ggtttccgca cctcccgtaa tacccgcagt cgtcaaatcc gcattcagca
    18841 ctgcacaaat agatcaatgg gtcgcacccg tcgcgctgta tcccgacgcc ctactttcgc
    18901 aggtgctgat ggcatcaacc tatccgacaa acgttgctca agcagtgcaa tggtcgcacg
    18961 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19021 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19081 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19141 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19201 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19261 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19321 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19381 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19441 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19501 acgcactatt cagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19561 atgatgatta tcatcaccac gatggcggtc atcgtgacag taatggctgg caacacaacg
    19621 gcgacaacat caatatcgac gtcaacaatt tcaacagtat caccggtgag catcttactg
    19681 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    19741 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    19801 tacctgctcc aacacgcgac agccagcgtc aggcgacagc aagtcagttt cagcaacgaa
    19861 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    19921 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    19981 tgacccagca gcaaaaggac gccgctcgtc agcgttatca gtcggcctct cctgagcagc
    20041 gccaggcagt ccgcgagaaa atgcagacta acccgcagat ccagcagcga agggaggcag
    20101 cgcgccagcg tattcagtcc gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc
    20161 agactaaccc gcagaaccag cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa
    20221 ctcccgaaca gcgccaggtg tttaaggaaa aagtacagca gcgcccactg aaccaacagc
    20281 aacgtgataa cgcccgccag cgtatacaat cggcatcacc tgaacaacgt caggtttttc
    20341 gggagaaagt tcaggagagc cgcccacaac gtctaaacga cagtaaccat actgtcaggc
    20401 tgaataacga gcaacggtca acagtacgcg aacgtctctc tgaacgtgga gcaagaagac
    20461 tggaaaggta aattgcagac gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg
    20521 gatgttccgg caaacgaaaa attacttctt cttcgctttc gggttcgggt tcggcaggtc
    20581 ggtaatgcta ccttcgaaca cttctgccgc caggcccaca gactcgtgca gagtcgggtg
    20641 cgcgtggatg gtcagtgcga tgtcttcagc atcacaaccc atttcgattg ccagcccgat
    20701 ttcacccagc agctcgccgc cgttggtacc aacaatcgca ccaccgatca cgcggtgaga
    20761 ttctttgtcg aaaatcagct tggtcatacc gtctgcgcag tcagaagcga tagcacgacc
    20821 agaagcagcc cacgggaagg tggcggtttc atagctgatg cctttctctt tcgcttcttt
    20881 ctcagtcaga cctacccatg caacttctgg ttcggtatag gcgatggacg ggataacttt
    20941 cggatcgaag tagtgtttct taccggcgat aacttcagcg gcaacgtgac cttcgtgaac
    21001 acctttgtgt gccagcatcg gctgaccgac gatatcgccg atagcaaaga tgtgcggtac
    21061 gttggtacgc agctgtttgt caacgcggat gaaaccacgg tcgtcaactt ccacgccagc
    21121 tttgcctgcg tcgaggtttt taccgttcgg cacacgacca atcgctacca gcacggcgtc
    21181 gtaacgctgc ggttcagctg gtgctttttt gccttccatc gtcacataaa taccgtcttc
    21241 tttcgcttca acggcggtaa ctttggtttc cagcatcagg ttgaatttct tgctgatacg
    21301 cttggtgaag actttaacga tgtctttgtc agctgccggg ataacctggt cgaacatttc
    21361 aaccacgtca atctgtgaac ccagcgcgtg gtaaacggtg cccatttcca gaccgatgat
    21421 accgccaccc attaccagca ggcgttctgg cacttctttc agttccagcg cgtcagtgga
    21481 gtcccagata cgcggatctt catgcggaat aaacggcagt tggatcgggc gagaacccgc
    21541 tgcaatgatc gcattgtcga agttgatcac ggttttgccg ttctcacctt caacttccag
    21601 ggtgttagcc ccggtgaatt tacccagacc gttgaccact ttgactttgc ggcctttcgc
    21661 cataccagcc agaccaccgg tcagctgatt gatcactttc tctttccagg tacgaatctt
    21721 gtcaatatcg gttttcggtt cgccgaagac gataccgtgt tcagccagcg ctttggcttc
    21781 ttcgataact tttgctacgt gcagcagtgc tttagaaggg atacagccga cgttcaggca
    21841 aacaccgcca agggtgttgt aacgttctac gattacggtt tccagaccta aatcagcgca
    21901 acggaaggca gcggagtaac ctgcggggcc tgccccaagt accacgacct gagttttgat
    21961 ttcagtactc atcatgacct ctatatattt atctccggcg gtcatacccg tcgtctttca
    22021 ggccgcagcg gcgttagctt cacgccctca ccccagtcac ttacttatgt aagctcctgg
    22081 ggattctggc gcttgctacc ttgctgcacc cagaaatcca tagggtatgt gaagtaatac
    22141 cctaaccacc accgggtcgt tctatccgtc ggtattttac aaaattgtta acaattttta
    22201 aacaacaaac ggcaaccgat ttgtctattc gctaataacc tcaatacatt catgagatta
    22261 ccagaaaaaa gccggccgtt gggccggctc ttttacttac atcaccagac ggcgaatgtc
    22321 agacagcgtg ttgttaatga tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg
    22381 gtggtcgaag gagagagaaa tcggcagcat cagacgcggc acgaactctt taccattcca
    22441 caccggctcc atcgcggact tggaaacgcc gaggatagcc acttccggcg cgttcacaat
    22501 cggcgcgaag tgggtagtac ccaggccgcc gatgctggag atggtgaagc aaccgccctg
    22561 catttcgccc gcagtcagct taccgtcacg cgctttctta gaaatagtca tcagctcgcg
    22621 agacagctcg atgatgcctt tcttgttgac gtctttgaat accggaacaa ccagaccgtt
    22681 cggggtatcc accgccacac cgatgttgat gtatttcttc agggtcagac gctgaccgtc
    22741 ttccgacagc gaactattga agcgaggcat ctgctcaaga gctgcagcaa cggctttcat
    22801 gatgaagaca accggggtga tcttcacatc cagcttacgt ttcgccgctt cttcgttctg
    22861 ctgtttacgg aacgcttcca gctcggtgat atcggttttg tcgaagtggg taacatgcgg
    22921 gatcatcacc cagttacggc tcaggttagc accagaaatt ttctggatac ggcccagttc
    22981 cacttcttcg atttcgccaa acttgctgaa gtccaccttc ggccacggca gcatgccagg
    23041 gataccaccg ccagtcgccg ccggagctgc ttctgcacgt ttgatagctt ctttcacgta
    23101 agcctgaacg tcttcgcgca ggatacgacc tttacggcca gtgcccttca ctttcgccag
    23161 gttaacgcca aactcgcgtg ccagacggcg gatcagcggg gtcgcgtgaa cgtaagcgtc
    23221 gttttcagca aattcagatt tgccttccgc tttcgcagcc ggagctgctg ccggagcttc
    23281 agcttttgct gccggagccg gcgctgccgc ttcctgtttc gcaggagctg ccgcaggtgc
    23341 tgcgccttca acttcgaaga tcataatcag cgaaccagtt ttcactttat cgccaacgtt
    23401 gactttcagt tccttcacga cgcctgcaaa cggagccgga acttccatag aagctttatc
    23461 gccttctacg gtaatcagtg actgttcagc ggcaactttg tcgcccactt tcaccatcac
    23521 ttcggtcact tcaacttcgt caccgccgat atccggaacg ttaacttctt tcacgccagc
    23581 tgctggtgcg ggggtcgctg ccggagtcgc ttcctgttta gccgccggag ctgccgcgcc
    23641 tgcttcaccc gcgacttcga agaccataat cagcgagccg gtagacactt tgtcacccac
    23701 gttcactttg atctctttca cggtacctgc aaacggagcc ggaacttcca tagaagcctt
    23761 gtcgccttct acggtgatca gcgactgttc agcttcaact ttatcgccaa ctttcaccag
    23821 gatttcggtc acttcaactt cgtcgctgcc gatatccgga acgttaacgt cttttgccgc
    23881 cgcagccgcg ggtgctgcta ccggagctgc ttctttcttc tcttctgcct gagcaggtgc
    23941 agcgtctgct gcaccgtcgg cggaatcgaa aatcataatc agtgcgccgg tctgggtttt
    24001 atcgccaaca gagactttga tctctttaac gatacccgcc tgcggagatg gaacttccat
    24061 agaggctttg tcgccttcta cggtgatcag cgactgttcg gcttcaactt tgtcgcccac
    24121 tttgaccagg atctcggtga tttcaacttc atcagccccg atgtccggta ctttgatttc
    24181 gatagccatt attcttttac ctcttacgcc agacgcgggt taactttatc tgcatcgatg
    24241 ttgaatttgg cgattgcgtc agcaaccact ttcttatcga tttcgccacg tttagccagt
    24301 tcgcccagcg ccgcaaccac gacataagaa gcatcaactt cgaagtggtg acgcaggttc
    24361 tcacggctgt cggaacgacc gaagccatca gtacccagta cgcgatagtc gtcagccggt
    24421 acgtaagtac ggacctgctc agcgaacagt ttcatatagt cggtagatgc cactgccggc
    24481 gcgtcgttca tcacctgagc gatatacgga acgcgcggag tttccagcgg atgcagcatg
    24541 ttccagcgtt cacaatcctg accatcacgc gccagctcgg tgaaggaggt cacgctataa
    24601 acgtcagaac ctacgccgta atctttcgcc aggatctctg ccgcttcacg gacgtgacgc
    24661 aggatggaac cggagcccag cagctgaact ttacctttgc taccttcaat ggtttcgagt
    24721 ttgtagatac ctttacggat accttcctca gcaccttccg gcattgccgg catgtggtag
    24781 ttttcgttca gcgtagtgat gtagtagtaa acgttctctt gtttttcacc gtacatacgc
    24841 tccagaccgt catgcataat gacagcaact tcgtaagcgt aagccgggtc gtaagagata
    24901 cagttcggga tagtcagcga ctgaatgtgg ctgtgaccat cttcgtgctg cagaccttca
    24961 ccgttcaggg tggtacgacc ggaagtaccg ccgatcagga agccacgcgc ttgctggtct
    25021 ccagccgccc agcacagatc gccgatacgc tggaagccga acatcgagta atagatgtag
    25081 aatgggatca tcggcagatt gttcgtgctg taagaggtcg ccgctgccag ccaggaacac
    25141 cctgcgccca gctcgttgat cccttcctgc agaatctgac ctttctcgtc ttctttatag
    25201 taagcaacct gctcgcggtc ctgcggggtg tactgctgac cgttcgggct gtaaatacca
    25261 atctgacgga acagaccttc cataccgaaa gtacgcgctt cgtcggcgat gatcggtacc
    25321 agacgatctt tgatcgactt gttcttcagc atcacgttca gagcacgaac gaaagcgata
    25381 gtggtagaga gctctttgct ctgctcttcc agcagcgcgc cgaagtcttg caggctcggc
    25441 agctcaagct tctcggtgaa gttcggctga cggcttggca gataaccgtg cagtttctga
    25501 cgctgagcgt gcagataggt atgctcttca gaaccttccg ggaaggtgat gtacggcagt
    25561 ttttcgatat ctgcatcaga caccggcaca ttgaaacggt cgcggatgtg acgcacgccg
    25621 tccatgttca ttttcttaac ctggtgcgcg atgtttttac cttcagccgc gtcgcccatg
    25681 ccgtaacctt taatggtatg agcaaggatt actgtcgctt tgcctttggt ttcctgcgct
    25741 ttcttgaatg cagcgtagat tttcttcgga tcgtgaccac cacggttcag tgcccagatc
    25801 tgctcgtcag tccagtctgc aaccagtgct gcggtttcag gatatttacc gaagaagtgt
    25861 tcacgaacgt acgcaccatc tttcgatttg aaggtctggt agtcgccgtc aacggtttcg
    25921 ttcatcagct ggatcagttt accgctggta tctttacgca gcagttcatc ccaacggcta
    25981 ccccacatca ctttgatcac gttccagcca gcaccttcga agatgccttc cagttcgttg
    26041 atgatcttgc cgttaccggt gaccgggccg tcaagacgct gcaggttaca gttgataacg
    26101 aaaaccaggt tatccagttt ttcacgggta gcgatggtga tcgcaccttt ggattccggt
    26161 tcgtccattt caccgtcgcc gaggaacgcg taaacggtct gtttagaggt atctttcagg
    26221 ccacggtgtt ccagatattt caggaattta gcctggtaaa tagcaccaat cggacccaga
    26281 cccatagata cggtcgggaa ctgccagaat tccggcatca gtttcgggtg cggataggaa
    26341 gagaggccat tgccgtgaac ttcctgacgg aagttatcca gctgctcctg agtcagacga
    26401 ccttccagga aagcacgggc gtaaacgccc ggggagatgt ggccctggaa gtaaaccagg
    26461 tcgccgccat cctgctcgtt gcgtgcacgg aagaagtggt taaagcacac atcataaatg
    26521 gttgcggaag actggaagga cgccatgtgg ccgcccagtt cgaggtcttt tttcgacgca
    26581 cgcagcaccg tcatgatggc gttccagcgg atagctgaac gaatacggcg ttccagttcc
    26641 agattacccg gatactccgg ttgttcttca acggggatgg tgttgatgta gttgctgata
    26701 cctgtgcctg cggctacgtt tacaccgcct ttgcgggctt cagcaagcag ttggtcgatc
    26761 agatactgag cacgctcaac accttcttca cggatgaccg attcgatcgc ctggagccag
    26821 tcgcgagttt cgatcggatc cacgtcattt gggaaacgtt ctgacatggg ttattcctta
    26881 tctatctaat aacgttgagt tttctggaac ctgtcccatt gaactctcgc cagaaagctc
    26941 aataagacag gttctacgtt tagttaccgc gcttttatat gcgcttgatt tacaacatct
    27001 tctggataat ttttaccaga aaaatcacta attctttcgt tgctccagac gacgcagaga
    27061 acgctcacgg cggctctctt cacggcttct gtcgagcaaa atttcttcga taaaggccag
    27121 atggcgatgc gatgcttcgc gcgcttcttc cggcttaccg gccataatcg cttcaaatat
    27181 gcgggtgcgg tgactactca ccagcggcag catctcgcga cgcgaataga gcaattcgaa
    27241 gttctggcgg acattctggg ccaacatcgg ctccatacac cttagcagat gaagcagaac
    27301 cacattgtgg gccgcttcgg tgacggcaat ctgatactgg agtacggcgt ttgattccgc
    27361 gtccagatcg ccagactgct gcgccagctc tatggcgtgg tggagttcac ggatgcgttc
    27421 cttgtcttca tcggtactac gcagcgcggc gtaataagcg gcgatacctt ccagggcgtg
    27481 tcgtgtttcg agcaagtcat actgtgattc aggatggtcg gagagcagct ccaccagcgg
    27541 atcgctgaag ctttgccata ggctgctctg gacaaaagtg ccgccaccct ggcgacgaag
    27601 caacaagccc ttcgcttcga gacgttgaat cgcctcacgc aaggagggac gggagacgtc
    27661 gaactgtttt gccagttcgc gttccggtgg gagtttttcg cccgggcgga gagtgccttc
    27721 gaggatcaaa aactccagtt gctgctcaat cacatcggag agttttggtt ggcggatttt
    27781 gctgtaggcc atgagttcct gtcttaagcc acttgccgaa gtcaattggt cttaccaatt
    27841 tcatgtctgt gacgctaaag taacaaagta ttcaccttat gtccatacag gttttgattg
    27901 aaatcatgaa actgtgcaca ttttaacaac ttgacatata taacgtttca aagttgtaac
    27961 tatgcacaaa tgtagacttt acgtaggaaa gggatttttt aacgattatt aaactataaa
    28021 aatccgaatt gaaccgattc acttaccaat tttttgattt ttaagtcaat aaaaacgaat
    28081 ttaaattcac tctgcatatt gagaggggtt gaggctgagc tttacaaacg gtttcttttt
    28141 aagcaactca tcttcaacca tgcataaagc gggtgcattc gctgccgcat accattattc
    28201 ttgatctgac ggaagtcttt ttgtaacaat tcaaacttcc ttgatgtaaa taaatcaata
    28261 caacaaacgg aattgcaaac ttacacacgc atcactgcgt agatcaaaaa aacaatcacc
    28321 gcacgaggtt tcatgatgga aggtcaacag cacggcgagc agctaaagcg cggccttaaa
    28381 aaccgccata ttcagcttat cgcgctgggt ggcgcgatag ggacagggct attcctgggt
    28441 agcgcctccg taatacagtc cgcagggcca gggattatcc tgggttacgc cattgctggt
    28501 tttatcgcct ttctgatcat gcgtcagctg ggtgaaatgg tggtcgaaga acctgtcgca
    28561 ggctccttta gccactttgc ttataaatac tggggcagtt ttgccggttt cgcctctggc
    28621 tggaactact gggtactgta cgttttagtt gccatggcag agctgactgc cgtgggtaaa
    28681 tacattcagt tctggtatcc ggaaatccca acctgggttt ctgccgccgt attctttgtg
    28741 gtgattaacg ccatcaacct gaccaacgtt aaagtgtttg gcgagatgga gttctggttt
    28801 gccattatca aagttattgc ggtagtagcg atgatcatct tcggcggctg gctgctgttc
    28861 agtggcaacg gcggcccgca ggcgagcgtt agcaacctgt gggatcaagg tggtttcctg
    28921 ccgcacggct tcaccggact ggtgatgatg atggcgatta tcatgttctc gttcggtggt
    28981 ctggaactgg tggggatcac cgcagcagaa gctgataacc cggagcaaag tatcccgaaa
    29041 gcaactaacc aggttatcta ccgcatcctg attttctata ttggttcgtt agccgttctg
    29101 ctctcactga tgccgtggac ccgcgttacc gccgatacca gtccgtttgt gctgatcttc
    29161 cacgagttag gcgatacctt tgtggcgaat gcgctgaaca tcgtggtact gactgcggcg
    29221 ctctccgtgt acaacagctg cgtatattgc aacagccgta tgctgtttgg tctggcacaa
    29281 caggggaacg cgccaaaagc gctggcgtct gtcgataaac gcggcgtacc ggttaacacc
    29341 attctggtgt ctgcgctggt tacagcattg tgcgtattga ttaactatct tgctccggaa
    29401 tccgcatttg gcctgttaat ggcactggtg gtatccgcac tggtgatcaa ctgggcgatg
    29461 atcagtctgg cgcatatgaa gttccgtcgc gccaagcagg aacaaggcgt ggtaactcgc
    29521 ttccctgctc tgctttatcc gctgggtaac tggatctgcc tgctgtttat ggcggcggta
    29581 ctggtgatta tgctgatgac cccaggaatg gcgatttcgg tatacctgat cccggtatgg
    29641 ctgatcgtgt taggtatcgg ctatctgttt aaagagaaaa ccgccaaagc cgtaaaagcg
    29701 cattaatctc tctacgccct cacccgtaca gggtgagggc aataatctct acaccaacgc
    29761 cccgtaaatc gtcagtagcg caatcaccac cacgaccacg aacgaggttt tcttcgccat
    29821 tgaaaccgct gccttcggcg tctccacctt atcgacatgc ggttcacgcg ccagagagaa
    29881 ctgcgccaga cgcgttaata cctgatattg cgaagtatgg aaatcaccca gcgaagcaaa
    29941 ccaggccggt aacgctttct caccatgacc gatcaaggca tataccacac ccgcaagacg
    30001 aaccggcacc caatccagta catgaagcac tgcatcaatg ccagactgta aacgatgatg
    30061 cggcgtctgg tatcgcgcca gccagtattg ccatgcacgc aagaacgcat accccatcag
    30121 cgtaacgggt ccccaggttc cccccacaat cagccagaac agcggtgcaa gataaaaacg
    30181 aaagttaatc cacagcaatg cattttgcag ctcacgcaaa tactcacgtt cgtcgcagcc
    30241 tgccgggacg ccgtgaatca tggtgagttc gccagccatc gtggcacggg catggctatc
    30301 attacgtgaa gcagctgtca gataagcatg ataatgaaga cgaactttac ctgcgccaat
    30361 acacagcaaa ccaatcagca gccacaccag cagcgtggga acgttgaaca atactccctg
    30421 caatgcgcgt aacagtaaaa aagtcacacc catcgcaata atggtcatgc ctaacgtgcg
    30481 cccgagagaa aaatgtttca cccgccgaaa gaacgcttca agacgatgat caagctgcca
    30541 gtgctcgccc aacttaaaca ggcgctcgaa aattaacacc agtaaggttg taaatagcgt
    30601 catgttgtct ccttgctgac cagcgcacga aaccgtgccc aatcaaatgc aggaccggga
    30661 tcggttttcc gatccggcgc aatatcacaa tggcccgtca tgttattagc gatatcggga
    30721 tagcgatcta tcagtgcccg cgtaaccgcc gcaagctgtt gatactgcgc atcggtatac
    30781 gccagagtat cggtgccttc aagctcaatc ccaatagaaa aatcattgca gcgttcacgc
    30841 ccctgatact gagagactcc cgcatgccag gcacgtttat cgaaaggaac atactggact
    30901 atttcaccat cacggcgaat caaacagtga gcggagacgc gcaaatgggc gatctcagca
    30961 aagaaaggat gtgcctgcgg atcaatagtt ccagtgaata atgcgtcgat ccacggaccg
    31021 ccaaactcgc ctggcggcag gctaatattg tgcaccacca gcagggtggg tgtttcgtca
    31081 tccaggcggc aatcgtaatg tggtgaggga acgcggcgcg cgccaaccag ccacccctgt
    31141 tctaacaaca tgcaggatct ccttatatgt ggtgctaata cccggttcag agtagcatgt
    31201 ttctacctta tgattcgtta gctatctgga gttttaacat gccgcctcgc cgctacaacc
    31261 ctgacacccg acgtgacgag ctgctggaac gcattaatct cgatatcccc ggcgcggtgg
    31321 cccaggcgct gcgggaagat ttaggcggaa cagtcgatgc caacaatgac attacggcaa
    31381 aacttttacc ggaaaattct cgctctcatg ccacggtgat cacccgcgag aatggcgtat
    31441 tttgtggcaa acgctgggtt gaagaggtgt ttattcaact ggcaggcgac gatgtcacca
    31501 taatctggca tgtggatgac ggcgatgtca tcaacgccaa tcaacccttg ttcgaacttg
    31561 aaggcccatc ccgcgtgctg ttaacgggcg aacgcactgc gcttaatttt gtgcaaaccc
    31621 tttcaggagt tgccagtaag gtacgccact atgtcgaatt actggaaggc accaacacgc
    31681 agttgttgga tacgcgcaaa accttacctg gcctgcgttc agctctgaaa tacgcggtac
    31741 tttgcggcgg cggagcgaat caccgtctgg ggctttctga tgccttcctg atcaaagaaa
    31801 accatattat tgcctccggc tcagtgcgcc aggcggtcga aaaagcgtcc tggctgcatc
    31861 cggatgcgcc agtagaagtc gaagtagaga atctggaaga acttgatgag gccctgaaag
    31921 ccggagccga tatcatcatg ctggataact tcgaaacaga acagatgcgc gaagccgtca
    31981 aacgcaccaa cgacaaggcg ctactggaag tgtctggcaa cgtcactgac aaaacactgc
    32041 gcgaatttgc cgaaacgggc gtggacttta tctccgtcgg tgcgctaact aaacacgtac
    32101 aagcactcga cctttcaatg cgttttcgct aattgctgac agatttatcc gctccatcgc
    32161 gggcggataa tctccccttc cccaactttc ttcgtaacgc ctcgcaaatt tcttgtcttt
    32221 cgctgcaacg aaataaaaaa cctcggaatc tgctttcccg tttcgttttt tgcccctcgc
    32281 ctgtgctgat ggcgtttgcc aaagtagcac caaccaaatc aaggagcgaa acagatggac
    32341 aagcaacgcg gttttacact tatcgaactg atggtggtta ttggcatcat tgccatttta
    32401 agcgccattg gtattcccgc ttatcaaaac tacctgcgca aagccgcact caccgacatg
    32461 ctacaaacct ttgtgcctta ccgtaccgcc gtagagttgt gcgcgctgga acatggtgga
    32521 ttagatacct gcgacggtgg cagcaatggc attccctcgc ctaccaccac ccgctatgtt
    32581 tcagccatga gtgtggcaaa gggcgtggtg tcgctgaccg ggcaagaaag tctcaatggg
    32641 ctaagcgtcg tcatgacgcc aggttgggat aacgcaaacg gcgtcaccgg ctgggcgcgc
    32701 aactgcaata ttcaaagtga cagcgcattg cagcaagcct gcgaagatgt cttccgcttt
    32761 gatgacgcca actaaggagc ggcaatgaat attccacagc tcacggccct gtgcctgcgt
    32821 tatcagggag tcttgctgga tgccagcgaa gaagtggttc atgttgcggt ggtcgatgcc
    32881 ccctcacatg agttgctgga cgcattgcat ttcgctacca ccaaacgtat tgagatcacc
    32941 tgctggacgc gccaacaaat ggaaggtcac gccagtcgca cacaacagac attgcccgta
    33001 gctgttcagg agaagcatca gcccaaagca gagttgctga ctcgaacgtt acaatctgcg
    33061 ctggaacaac gcgcgtctga tattcatatc gaaccagcgg acaatgccta ccgcatccgc
    33121 ttgcgtatcg acggcgtatt gcatccttta ccggatgttt caccggatgc cggagtcgca
    33181 ttaacagcca gattaaaagt gctgggaaac ctggatattg cggaacatcg cctgccgcag
    33241 gacgggcaat tcactgtcga actggcagga aacaccgtct catttcgtat tgcgacctta
    33301 ccatgtcggg gtggtgaaaa ggtggtatta aggttgttac agcaggtgag ccaggcactg
    33361 gatgttaaca cgctgggaat gcagccgtta caactggcgg actttgctca tgccttgcaa
    33421 caaccacagg gactggtgct ggtaactggc cctacaggca gcggcaaaac ggtcacgctt
    33481 tatagtgccc tgcaaacgct gaataccgct gacattaata tttgtagcgt cgaagatccg
    33541 gttgagatcc ccatagccgg actaaaccag acgcaaatcc atccgcgtgc cgggctcacc
    33601 tttcagggtg ttttgcgtgc gttattgcgc caggatcctg acgtcatcat gatcggagag
    33661 atccgcgatg gcgaaacggc agaaattgcc attaaagccg tgcaaaccgg tcacctggtg
    33721 ttgtccacgc tacacaccaa ttccaccact gaaacgctgg tacgtttaca gcaaatgggg
    33781 gttgcccgct ggatgctatc atcggcgctt acgctggtaa tagcccagcg tctggtacgt
    33841 aaactttgcc cacattgtcg ccgacagcaa ggggagccca tccatattcc agacactgta
    33901 tggccgtcgc cgctgcccca ctggcaggca cccggttgtg tacattgcta ccacggtttt
    33961 tatggtcgta ccgccttgtt tgaagtcctg cccattacac cagccatacg ccagcttatt
    34021 tccgctaata ctgaagttga atcgctggaa acgcacgccc gacaggcggg tatgcgtacg
    34081 ctttttgaaa acggctgcct ggccgtggag caaggcttaa ccacctttga agagttaatc
    34141 cgcgtactgg ggatgccgca tggcaagtaa gcaactctgg cgctggcatg gcataaccgg
    34201 cgacggcaat gcgcaagatg ggatgctatg ggcagagagc cgtactttac tgcttatggc
    34261 actacagcaa cagatggtta ccccactaag cctgaagcga atcgccatca attctgcgca
    34321 gtggcgagga gataaaagcg cggaagtcat tcatcaactg gcgacgctac ttaaagccgg
    34381 gttaacgctt tctgaagggc tggctctgct ggcggaacag catcccagta aacaatggca
    34441 agcgttgctg caatcgctgg cgcacgatct cgaacagggc attgcttttt ccaatgcctt
    34501 attaccctgg tcagaggcat ttccgccgct ctatcaggcg atgatccgca cgggtgaact
    34561 gaccggtaag ctggatgaat gctgctttga actggcgcgt cagcaaaaat cccagcgtca
    34621 gttgaccgac aaagtgaaat cagcgttgcg ttatcccatc atcattttag cgatggcaat
    34681 catggtggtt gtggcaatgc tgcattttgt tctgccggag tttgccgcta tctataagac
    34741 cttcaacacc ccactaccgg cactaacgca ggggatcatg acgctggcag actttagtgg
    34801 cgaatggggc tggctgctgg tgttgttcgg ctttctgctg gcgatagcca ataagttgct
    34861 gatgcgccgc ccgacctggc ttatcgcgcg gcagaaattg ctgttacgca tcccgattat
    34921 gggttcactg atgaggggac aaaaactcac gcagatcttt acgattctgg cgctgacaca
    34981 aagtgcaggc attacttttt tacagggcgt agagagcgtc agagaaacaa tgcgctgccc
    35041 gtactgggtg caacttctga cacaaatcca gcacgatatc agtaacggtc atcccatctg
    35101 gctggcgcta aaaaatgccg gtgagtttag cccgctctgt ttgcaattag tgagaacagg
    35161 agaggcatcc ggctcgctgg acctcatgtt agacaacctc gcccatcatc atcgggataa
    35221 cacaatggcg ctggcggata acctcgcagc cttactggaa ccggcgttgc tgatcataac
    35281 gggaggaatt atcggtacgc tggtggtggc aatgtatctg ccaattttcc atttaggcga
    35341 tgcgatgagt gggatgggat aacgctggcg tgttgctcca cgccagcgtt gggagattac
    35401 aggttgttga agatgcggtt ttcttgttcc agcacacgaa taaacgtggt gcgcttggtc
    35461 agctctttca ggcgtgaagc cccaacgtat gtacaagctg aacgcaggcc gcccaaaata
    35521 tcacgcgcgg tattttcaac cgggcctcgc agcggcagct tgacggtttt accttctgct
    35581 gcgcggtatt ccgcaacgcc gccaacgtga cgtttcatcg cagactcgga gctcatgccg
    35641 tagaacagca taaatttctc gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg
    35701 cccgccagca tgccgccaag catgacgaaa tcggcaccgc cgccaaaggc tttcgccaca
    35761 tcgcccggcg tggtacagcc accgtcgctg acgatcattc cgcccagacc gtgcgccgca
    35821 tcggcacatt cgattaccgc agaaagctgc ggataaccga cgcctgtttt gacgcgagtt
    35881 gtacaaacag aacctgggcc aatgccaact ttaacgatat cagcaccgga gaggataagc
    35941 tcctcacaca tttcaccagt cactacgttg ccagcacaaa tggttttggt cggccacgct
    36001 tcacgcgctt tcgcaacgaa ctgcacgaag tgttcggaat aaccattcgc cacatcaata
    36061 caaacgaagt ttaatgccgg gttcaggtcg agaatctgtt tagttttttc gaaatccgca
    36121 tcagacgtgc cggtagaaac catcacatgt ttcagcacat cagcggaaga attgttgata
    36181 aacgcttgcc actcttcgac agaaaagtgt ttatgcacag cagtcagaat atcaaaagaa
    36241 gccagcgcag aggccataga aaatgtgcct acggtgtcca tatttgcggc gataatcggc
    36301 acgccggacc agctctgacc tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg
    36361 gaacggcttt taagagtgga gcgtttaggg cggatgagaa cgtctttaaa acctaacttc
    36421 agatcttctt caatacgcat gtgcggattc ctggggttaa tggctataag ggtaagagcg
    36481 caactccagt gacgctatca tacgcactaa tcaatgcggc gcaagactgc gaaattgtct
    36541 ttttttacgc tacaatccca taaatttacc tggtaaatag tgagcgaacg tttcatcaat
    36601 ttcactttcg cgcgctcatt tttaacgttt tgataatcat gatgaattcc cgggaatata
    36661 aattatgggg tatatagttg ccttaacggg aggcattggc agtggtaaga gtaccgttgc
    36721 caatgcgttt gctgatctcg gaattaacgt cattgatgcc gatattattg cgcgtcaggt
    36781 ggttgaacca ggtgcacctg cgctacatgc cattgctgat cactttggcg aaaacatgat
    36841 tgctgctgat ggaacattgc agcgccgggc cttgcgcgag cggatcttcg ccaacccgga
    36901 agagaaaaac tggcttaacg acctgctgca tccgctgatt cagcaagaga cgcaacacca
    36961 gatccagcaa gcaacttccc cctatgtact gtgggttgtg ccattgctga tagaaaactc
    37021 actgtataaa aaagcgaatc gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa
    37081 gcgcaccatg cagcgcgatg atgtaactcg cgaacatgtc gaacaaatcc ttgctgctca
    37141 ggcaacgcgc gaagcccgcc ttgccgtggc agatgacgtc attgataata acggcgcacc
    37201 ggatgctatc gcatcggatg ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca
    37261 gtttgtctca caggaaaaac cgtaatgcag acccaggtcc tttttgaaca tccactaaat
    37321 gaaaaaatgc gtacatggct gcgcattgag tttttgattc agcaactcac cgttaattta
    37381 cccatcgttg accacgctgg cgcgctgcat ttcttccgta atgtcagtga attactggat
    37441 gttttcgagc gcggcgaagt ccgcactgag ctgttgaaag aacttaaccg gcagcaacgt
    37501 aaactccaga cctggattgg cgtgcctggc gtggaccaga gccgtattga agcattaatt
    37561 cagcagttaa aagcggcggg gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg
    37621 cgtgaagatc gtttgattgc tctggtgcgt cagcgactga gcatcccagg cggctgttgc
    37681 agctttgatt tacctacatt gcacatttgg ctgcatctac cccaggcgca gcgcgacagc
    37741 caggtagaaa cctggattgc cagcctgaac ccgctcaccc aggcacttac catggtgctg
    37801 gatttaattc gccagtcggc ccccttccgt aaacaaacca gcctgaatgg tttttatcag
    37861 gataacggtg gcgatgccga cttgctgcgc ctgaatctgt cgctcgattc acagctttat
    37921 ccgcaaattt ccggacataa gagccgtttt gccattcgtt ttatgccgct ggacactgaa
    37981 aacggacagg taccggaacg tctggatttc gaactggcct gttgctaagg agtaaaaatg
    38041 tcagaaacta ttacggtgaa ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc
    38101 agcccgtttc ggccattttg ctccaaacgt tgtcagctga tcgacctcgg agaatgggct
    38161 gctgaagaaa aacgaatccc cagcagtggc gatctttccg aaagcgatga ctggagcgaa
    38221 gagccaaagc agtgacattt gtctgatgcc tcacgtaggc ctgataagac gcggacagcg
    38281 tcgcatcagg catcttgtgc aaatgtcgga tgcggcgtga acgccttatc cgacctacag
    38341 acgcttaagc ttcgcaatta ccggttcatt ggctggcgga aaatcatcgg cattaagacc
    38401 gaccagcgac atccactcac cgggttgccc ttctttaccc cacggcaccc cttcccaact
    38461 ttcgaccagc cagaaccaca gagttatatg cctgtccggg aattcatatt ccagtttttc
    38521 aaatagcgaa aaatgttgtg gggtaatccc gacctcttcc tgaagttcac gcaccaccgc
    38581 ctgttccggc gtttcgccca tttcaatttt accgccggga aactccagtt tattcgccat
    38641 gtgcgcatct gctgcgcgac gtgttataaa gatttcattg ttctcgttgc gaataatacc
    38701 taccgcaatt tgcagctttt tcattgtctt aaacctataa aaaaggcgca gaatcctgcg
    38761 ccttttactt caactgttag cttttattgc aggcgaccat ggcactgctt gtatttttta
    38821 ccagaaccgc acgggcaagg atcgttacgt cctactttgc gttcaccggt ttgcgccgcc
    38881 agtgcagctg cggctgcaga gtcgtcatcc tgatggctaa gctgctgcat ttgtgctaaa
    38941 cgctcggctt ccatacgacg ctgttgttcc agctcctcaa cctcttcagg catacgtacc
    39001 tgaactttgc tcagcgtact gataacttca tatttcaacg actccagcat cgctgcaaac
    39061 atggagaacg attcacgttt gtattcctgc ttcggatctt tctgtgcata gccacgcagg
    39121 tggataccct gacgcagata gtccatcgct gccaggtgct ctttccacag ggagtcaagc
    39181 gtttgcagca tgacgccttt ctcgaagtga cgcatcatct cagcaccaac cacttcttct
    39241 ttacgctgat acacttcgat ggactgcgcc agaatgcgct cacgcagcgt ctcttcatgc
    39301 agttctggtt ctttatccag ccactcggca attggtaaat cgaggtcgaa atcgttcttc
    39361 agacgttcct gcagccccgg aatatcccac atttcttcca gcgactgtgg cggaatgtag
    39421 gcatcaatgg tggctttgaa cacatcttca cgaatgctgt tgatggtttc gctcacatcg
    39481 ctgacatcca gcagttcgtt acgctgggag taaatggcgc gacgctgatc gttagccacg
    39541 tcatcatatt ccagcagttg cttacgaatg tcgaagttac gactttcaac tttacgctgg
    39601 gcgttggcaa tcgctttagt cacccacggg tgttcaatgg cttcgcctag cttcataccc
    39661 agtttacgca tcatgccgga tactcggtcg gaagcaaaaa tacgcatcag cgcatcttcc
    39721 atcgacaggt agaaacggga agaaccagca tccccctgac gaccagaacg accgcgcaac
    39781 tggttatcga tacgacggga ttcgtgacgc tcggtaccga tgatatgcag gccacctgct
    39841 tccagtaccg catcgtgacg tacctgccag tcggctttaa ttttttcaat ttgctctgcg
    39901 gtcggatttt ccagcgcggc aacttctgcc tgccagctac ctccgagcac aatatctgta
    39961 ccacgacccg ccatgttggt cgcgatagtc accgcagccg gataacctgc ctgagcaaca
    40021 atcgccgctt cgttggcgtg gaatttggcg ttcaggacat tgtgcttaat accggctttg
    40081 gtcagttcgt ttgacaccag ctccgatttt tcgatggaga tagtacccac cagcaccggc
    40141 tggcctttcg cagtacgttc tttgatatct tcaatgatcg cctgaatttt ttccgcttca
    40201 gtcatgtaga ccaggtccgg cagatcttta cgaatcattg gacggttggt cggaacaacg
    40261 acggtatcca gtttgtagat ggagctaaat tcgaaagctt cggtatcagc agtaccggtc
    40321 atccccgcca gtttttcata cagacggaag tagttctgga aggtgatcga agccagcgtt
    40381 tggttttcgt tctggatctg cacaccttct ttcgcttcca cagcctggtg cagaccatcg
    40441 gaccagcgtc ggccctgcat ggtacgaccg gtgtgttcgt caacgatgat aacttcacca
    40501 tctttaacga tgtagtcgac gtcacgggta aacagcgcat gagcgcgcag cgccgccgtt
    40561 acgtggtgca tcagcatgat gttggccgga gagtacagag actccccttc atccatgatg
    40621 ccctctttca ccagcagttc ttcaatcagc accagaccac gttcggtcag gttcacctgg
    40681 cgagattttt cgtccaccga gaagtggcct tcgccctgga aggtttcgga gtcttctttt
    40741 tcctgacgga tcaggtgcgg aataatttta ttcacgcgtt tatacatttc cgagctgtct
    40801 tctgccgggc cggaaatgat cagcggtgta cgcgcttcat cgatcaggat ggagtccact
    40861 tcgtccacca gcgcatagtg cagtttacgc tgtacacgtt cttcagggct gaacgccatg
    40921 ttgtcgcgca ggtagtcaaa gccgtattcg ttgttcgtac cgtaagtgat gtcagctgcg
    40981 taagcttcgc gctttgccgg tgctggcatg cccggcaggt tgataccaac agtcaggcca
    41041 aggaattcaa acagcggacg gttgttttcg gcgtcacgtt gcgccaggta gtcgttgacg
    41101 gtaactacgt gcacgccttt accggttagt gcgttcaggt aagcaggcag cgttgcggtc
    41161 agggttttac cttcaccggt acgcatttcg gcgatgcagc gttcgttaag aaccataccg
    41221 ccgagtaact gaacgtcgaa gtgacgcata ccaaagacgc gcttacttgc ctcacgcacc
    41281 acggcgaaag cttccgggat cagattttcc agcacttcgc ctttttccag acgcgcacga
    41341 aactctgcgg ttttcccttt cagttcttca tcggagagtt tttccatctc cggttccatg
    41401 gcattgatga tgttgaccac tttgcgcatc cggcgcaggg tgcgatcgtt acgactaccg
    41461 aaaactttag ttaacaattt gattagcata ataaaatctc aaacgccccg cgttacggag
    41521 ttaataaaat gaagtaaagg tttattgttg ttaggtgagg cgttgagggc cagcacggat
    41581 gccttgcgcc tggcttatcc agacgggcgt gctgaatttt gcttgtgggg taaaatgcgc
    41641 ataatcaatg cgataaccct tttcagacgg cgtgccttcc tgggtcagca gcgcgctgag
    41701 cgtatccagt aatgcaagat gttgcgcctg aagaggcaaa gattcttcag caacgggcag
    41761 tgtttgcggt gccattgcga aagaaagatg acggattacc gtgcgaatag catgttgatg
    41821 ccagtaatca acggaatagt tcgaattcgg gcggcgcgtg ttcgcttcca gcaaggccaa
    41881 ttgaccaaag ttaactttgg ctgaaggctc gtggttgcgg gttgtcgctt ttgcgggcgc
    41941 gtttggttcg gcggcgttgc tgagcgcagg caaacctaaa ctcgccgcga ccatccctaa
    42001 taagagatgc ggccagaagt agcgtttacc aaactgtcgc cagcgcgtca gtattccact
    42061 cacgttattg ccatcccgat cgaattcaaa cgtatttagc gcacaaatct tatcattaaa
    42121 gcctgaagtc cacagcacga atgaagataa gttgtgcgta aaagtgctta agaaaacagc
    42181 gttcgcaccg ttttttatag ctcttataaa attcaaaaaa aaacgactgg ttcacctggc
    42241 cggagagagt gccagatttg ccagtcgaat tttatacgac agtataaatg tcgttatgcc
    42301 agtacagctg aaggcgcttt gaaggccaac ggcagttctg cgtcgtcctg gaaggtcaca
    42361 tattcccagg cttcctgttt cgccaggaca gcctgcagca gtttgttatt cagtgcatga
    42421 ccggatttat aagcggtaaa tgcaccaata atattgtgac cacacatgaa caagtcaccg
    42481 atcgcatcga gcattttgtg acgcacaaat tcgtcttcaa aacgcaggcc gtcttcgttc
    42541 agtacgcgat aatcgtcaac aacgatggca caatcgaagc tgccgcccag gcacaaacca
    42601 cgggactgca gatattcgat atcacgcatg aaaccgaacg tacgcgcacg gctgatctgg
    42661 cgcataaacg catcagcgga gaagttcatc gcatagcgct ggttgctgga atcaatagcc
    42721 ggatggttaa aatcgatggt gaaatccagc gaaaaaccat tgtacggctt aaattcagcc
    42781 cacttatcgc catcttcgac acgaacagtc tctttgatgc gaacaaattt tttggcgcag
    42841 ttcaactcgt cgataccggc gtcaagcagc aggtatacaa acggagcggc gctgccgtcc
    42901 atgatcggga tttccggcgc gttaacttcg ataacaatgt tatcgatgcc caggcccgcg
    42961 agagcagcat tgaggtgctc tacggttgaa atccgtacat catgctcgtt gaccagacac
    43021 gtacagagca tggtatcacg cacagatttg gcatcggccg ggaaatctac cggtggattc
    43081 aagtcggtgc gacgatagat gaccccggtg ttggccggcg cagggcgtaa cgtcagggtg
    43141 actttcttgc cggtatgtaa accgacaccc gtcgcctgaa cgatacgttt aagtgtcctt
    43201 tgtttgatca tcgtattatc tcgccaaatt acctatccaa ccgaagtgta ctatacattc
    43261 ggcgggccag tttagcacaa agagcctcga aacccaaatt ccagtcaatt cttaatcagc
    43321 ttgcttacgt aggaatgctg ggatatccag ataatccggc tctttcgcag tttgcggcgc
    43381 attgtcattc actactttag caaccggctt ctgctcctgg gtcagcggag ccatcccatg
    43441 ctgctggtag cgatccatca ctggctgctg aacctgctta ttggtcacca gagtgatttc
    43501 aggacgtttg tccatgccga tacctgtcgc aacaacggtt acgcgcagct cgtcattcat
    43561 atccgggtca agagaagtac cgataaccac agtcgcgttg tcggaagcaa atgcacggat
    43621 ggtgttacct accgtttcga actcatccag acgcaggtcg aagcccgccg tgatgttaac
    43681 cagcacgccg cgcgcgccag acaggtcgat atcttccagc agcggagaag agatagccat
    43741 ttcagcagct tcttccgcac ggtcttcacc gctcgccacg ccagaaccca tcattgcgta
    43801 gcccatttca gacattacgg tgcgtacgtc tgcaaagtcc acgttcatca aacccggacg
    43861 agtaatcagt tcggcgatac cttgcacagc gcctttcagt acatcgttcg ctgcgccaaa
    43921 cgcatccagc agggagatac cgcggcccag aactttcagc agtttgtcgt tcgggatagt
    43981 gatcagagag tccacatgct tggacagttc agtgatcccc tgctcggcga atgccatacg
    44041 cttcttgcct tcaaagttga aaggcttagt gacgacagca acggtcagga tacccaaatc
    44101 ttttgccact tcagcgacga ctggtgctgc acctgtaccg gtaccaccac ccatacccgc
    44161 agcaataaag accatgtctg caccttccag cgccgcacgc aatgcatcgc gatcctcatc
    44221 agccgcattg cggccaactt ctggattagc gccagcgccc agtcctttgg tgataccgct
    44281 accgatttga atcgtctgtc caaccgctgt tttacgcagc gcttgtgcat cggtatttac
    44341 cgcgaagaat tcaacacctt caatacgctc gcgcaccatg tgttcaacag cattaccgcc
    44401 gccgccgccg acgccgatga ctttaatcac cgcgtcattg gtaagttcca ttggttcaaa
    44461 catagtttct ctccgatttg tgcctgtcgc ctgaggccgt aatcatcgtc ggcctcataa
    44521 aaattaaaac tcttttcgca gccaactatt gagtcgcttg atccacgagc caactgatgc
    44581 tgtaacacgt ttttctactt cagcttcacc gttaagatgt gactctttcc catagtgaag
    44641 caatcccacc gccgtcgaat aatacggctc ctgagcataa tccgttaaac cggtaatgtt
    44701 cagcggcgcg ccgatacgca cttgcgtatg aaacacgcgc tgagcacagg ctgcaagacc
    44761 ttcgatctgc gctgcgccac cggttaatac aatgcctgcc gccaggtgat gtttaacccc
    44821 ttgttggcga agcttttcct gcaactgcaa tatctcttcg ttgaccaggt tgagcaactc
    44881 ggtatagcgc ggctcgatca cctctgccag ggtctgacgt tgcagactcc gcggcggacg
    44941 accgcctacg ctcggcactt ccacgctctc atcttttcca acgatggaac ccagcgcaca
    45001 accgtggcga actttaatcg cttcggcgtc gcttggcggc gtgccaaagg cgtaagcgat
    45061 atcactggtc acgacattgc cagcataagg aattacctta gtgtggcgta atgccccacc
    45121 ggtataaacg gcgatatcca ttgtaccacc accgatatcg acgacgcaga cacccagttc
    45181 acgttcatct tccgtcaata ccgaataact tgatgccagt ccggcaaata tcagttggtc
    45241 aactttcagc ccacaacgtt caaccgcttt gacgatgttt ttcgccatat cgttgtgaca
    45301 tgtgatcagg tgcacttttg cctgcatccg cacacccgaa agtcctaccg gattcttgat
    45361 cccttcctga tagtcaatcg catactcttg cgggatcaca tgcagcacac gatgctcatc
    45421 gcgcacacgc accgatttcg cggtatggac gacgttttcc acatcttctt gcgtcacttc
    45481 ttcttcagaa ataggcacca taccaatttc attctggcag ctgatgtgct taccagaaag
    45541 cgccagatat accgaagaga tctgacaatc tgccatcaat tctgcctggt caatggcgcg
    45601 ttgtacgcac ttgaccacgg attcgaggtc gttcaccccg cctttatcca taccacgtga
    45661 cgggcagctg cccacgccaa tgatattgac cataccgtcg ggcagaactt cccctactaa
    45721 agcggcaacc ttcgcggtac caatttccag tcctactacc agttttctgt ccgtcgcctt
    45781 gatcattgtt gttctgcctg tgcctgattt tgttgctgag tagattcctc tggcggcaag
    45841 ggcgcccagc ctactgccgc tccagagtca taacgcaaat caacgtagct aatccgtttg
    45901 ccatcggttt gcgcctgctg ctgtaaaacc ggataaagtt ctacaaagcg agccaaacgt
    45961 ttcatcgtat cgccccggcc aagattgagc ttaatatcgt tattcagcgt caactgccag
    46021 gaacgccgcg cggtcatcgc cgcttccttc agagtaaatc tgtcctttgc cagcatctgc
    46081 cccatttcgc gatagccctg caacacttca ttagcgctgc cttccgggcc atacagcatt
    46141 ggaagcacct gcttgctggt gcgatctggc ggcacgctga aggtgtttcc ttccgcgtct
    46201 accatgtgtt gatcattcca ccgcgcaatc ggcacatatt caaccagatg aatcttcaat
    46261 tcatcaggcc actgctttct gacgctcacc tgcttaatcc acggcaggcg ttgttctatt
    46321 tgcgtctgga tgatgttgac atcctgggtc ataaaggtac ccggctcacc caatgccagg
    46381 atcgactgcc ggatatcgtc attacgcgtg taatggcgtt caccggtcaa caccagcttt
    46441 gagagcggca ggcgttgcgc atcttccatc cagctcaaca cgacccagcc gctcaccaac
    46501 actgtcgtta aaacggtcag caggaaaagg atccccgcca gacgcgttcc attattgcgg
    46561 cgagaagaaa cctcttcttc gctgtttcgc gtgttcagag cagcctgcga catattagtc
    46621 cgccagttcc agaattcgta ctaccaactg cgagaagctc atacctgcct gacgtgccgc
    46681 catcggcacc aggctgtggc tggtcatacc cggtgaggta ttggcttcca gcagataaaa
    46741 ctgtccatca ctgtccagca taacgtcaat acgtccccat cctttgcaac ctaacgtcgt
    46801 ccatgctttc agcactaatg cctgcaaatt ggcctcttgt gacgctttca gacctgcggg
    46861 gcagaaatac tgtgtctcat cagagagata cttcgcctca taatcataga aggttccgga
    46921 cggttgaata cgtattgacg gtaaaatttc ttcaccgagt atcgcaaccg tgaactccgg
    46981 cccacttagc catttttcaa tcaatacttc ttcatcgtgc tgaaatgcca atcttaatgc
    47041 atcttgtaga gcattttctg ctactacttt tgacattccc acactggaac cttcgcggct
    47101 cggcttaacg ataaccggca aacccagagc agaaatttct gctaactgct tatcgctcag
    47161 gcctttttca aactctgcgc gggttaacgc tacccacggc gcgaccggta aaccggcacc
    47221 ttgccacaga agtttgctgc gtagtttatc tattgaaagc gcagatgcca tcactccgct
    47281 gccggtataa ggcaagccca tcagctcgag catgccctgc agcgtaccat cttcaccgcc
    47341 gcgaccgtgt agcgcgataa acactttctg aaaacccatc gacttcagtt gcgtcacgtc
    47401 gacttctttc gggtcgacag gatacgcatc aataccgcct tcacgcagcc cggctaacac
    47461 cgctgcgcca gaattcagag aaacttcccg ctcagcggaa gtcccgccca acaggaccgc
    47521 gattttatca gtcatgttgt tcttcctccg gagtttgcgg cttcagtttg atttcagcta
    47581 aagaacgggc aatttttccg atattaccag ccccctgaac gagaatcagg tcgttaccgg
    47641 ttaataccgg tgccagcatc tcggctacct gcgcagggtc cggcaccaga atgggatcaa
    47701 ttttcccacg tccacgaatt gtgcgacaca gcgaacggct gtccgctccc ggaattggcg
    47761 cttcgccagc cggatacact tccagcatca acagggtatc aacctgcgtc agcacattgg
    47821 cgaaatcatc atacaggtcg cgcgtacggg taaaacggtg cggctgaaac agcattacca
    47881 ggtttttatc cggccagcct gcgcgcgccg ctttaatggt ggcgtcaact tccgtcgggt
    47941 ggtggccgta gtcatcgacc agcattgccg taccgctttt accattcact ggctccagcg
    48001 ggaattcacc gaggaaatca aaacggcgac cagtcccctg gaagctctcc agcgcacgca
    48061 aaatagcctc gtcgtcaatt ccctcttccg tagcaaccgc aaccgcagct gcggcgttca
    48121 gcgcgttatg acggcctggc gcattcaggg tgacgcgcat cggctctttg tcctgacgca
    48181 gcagcgtaaa gtgcccctgc gggccaatct gctgataatc ttctacacgc acgtcggcat
    48241 cttcgctgaa gccgtaagtt gtggtctgac gtcccacacg cggtaacaat tcgcggatca
    48301 ccggatcatc aacacacatc accgcacgac cgtaaaaagg caggttgtgc agaaaattaa
    48361 taaaggtctg ttttaaattc tcaaagtcgc cctggtaggt atccatgtgg tcggcttcga
    48421 tattggtgac aatcgccacc atcggttgca gatgcaggaa cgatgcatca ctctcatctg
    48481 cttcggcaat caggtaccga ccatgcccca aacgcgcatg aacccccgcc gcttttacca
    48541 gcccgccgtt aacgaaggtt gggtcgagcc ccgcttctgc gtagatgctg gaaaccatcg
    48601 cggtagttgt cgttttgccg tgcgttcctg caatggcgat gccatgacga aaacgcatta
    48661 actcagccag catttcggca cgacggatca ccggaatacg cgcttcatga gctgcgacaa
    48721 tttccgggtt gtcggcagaa atggcgctgg aaacaacgac cacgctggca tcacgtacgt
    48781 tttccgggcg atggttgaaa taaatcgtcg cacccagatt cagtaactgc tgcgtgaccg
    48841 gatttggcgc taaatcggaa ccactgatct gataaccttc attggccaga acttcggcaa
    48901 taccgcccat accggcacca ccaatgccga caaaatgtat gtgccgaacg cgacgcattt
    48961 cgggcacgat ggaacgcagt tttgccaatt gttgtgtatt cattctttac gccattaact
    49021 tcttaaattc atacgatgca aaaggcatcg ctacaattac gcccgggcag cccggctcac
    49081 ttcatttgcc actcgctcgg tggcatccgg aatggatgca gcgcgggcgc gttctgccat
    49141 ggttaataag gtttctcgcg accacccggc cagggtgttg gcgacagcat ccacgctaag
    49201 ctgtggctgc tcgataattt tggctgcgcc cgctttttcc agcggtagcg cattccagta
    49261 ttgctggcgg tctttatgtt gaaacggcac aaacaacgcc ggtagtcctg ccgcggcgat
    49321 ttcactcaca gttaaagcac cggagcggca aacgacgaca tccgcccacg catacgccgc
    49381 cgccatatca tcaataaatt cagtcacttt atgctgcggt tgccccgctt cggcatacgc
    49441 ctgttcaacg gattgttgcg aacctttgcc gctctgatgc cagatagtga ctgaatcacc
    49501 cagtttcgca gcaacctgcg gcattgtctg gttaagaatg cgtgcgccct gggaaccacc
    49561 cactaccaac acacgaaccg gaccttcacg tccagccaaa cgttgctgcg gcaacggcag
    49621 cgccagcaca tcggtacgca ccgggttacc caccacttcc gcattaggga aagcgcctgg
    49681 aaacgcctgc atcactttgg tagcaatctt cgccagccat ttattggtta agcccgcaat
    49741 accgttttgt tcatgaagta caaccggaat gcctaacgac cacgcggcca gaccacctgg
    49801 acctgataca tagccgccca taccgagtac cacgtcaggt ttgtacgctt tcataatcgc
    49861 ccgcgcctga cgccagccgt tgaagatacg cagcggcgca gctatcagtg cttttatacc
    49921 ttttccacgc aaaccagaga tacgaatgaa atcaatttcg atgccatgtt ttggcactaa
    49981 gtccgcttcc atacggtcgg cagtccccag ccagcgaact tgccaaccct gagccattag
    50041 atagtgcgca accgccagtc ccgggaatac atgtccaccg gttccgcctg ccatcaccat
    50101 taatcgcttt ccttgaccac tcatcgtgaa cctcgtacaa acgcctgcgc tttctccaga
    50161 cgcgtttcat aatcaatacg caacagcatc atgatggctg tcgacataat cagtaagctc
    50221 gaaccaccgt aactgattag cggcaatgtc agacctttgg tcggtaacat ccccgccgcc
    50281 gcgcctacgt taaccagcgc ctggaagcta aaccagatgc caatagaaca ggcgagaaaa
    50341 ccggaaaaac ggtggtcaat ttctaatgct ttgcggccaa tcgacatcgc acgaaaagcg
    50401 acgaagaata ccattaaaag tgctagtacc acaccgacat aacctaattc ttcgccgata
    50461 atggcgaaaa taaagtcagt gtgcgcttcc ggcagatact ccagtttttg taccgaatta
    50521 cctaaacctt gcccccaaag ttcgccgcga ccaaacgcca tcagcgattg cgttaactga
    50581 tagccgctgc caaagggatc ttcccacggg ttccagaatg cggtaacacg gcggatacgg
    50641 tacggttcgg cgaggatcag caacacaacc gctgaaatgc ccataccgat aatggcaatg
    50701 aactgccaca atttcgctcc cgccaggaac aacatcgcca gcgtagtcac aaacaacacc
    50761 accaccgtac caaggtctgg ctgtgccagc agtaacactg ccaacaccag aatcacgccc
    50821 atcggtttca ggaagccgcg caggttatta cgtacttcgt cgcctttacg caccagatag
    50881 ttggcgatat agcaaaacag cgacagtttt gtcagctccg caggctggat acgcagcaaa
    50941 ccgagatcga tccaacgcga tgccccttta acagcgctac ctactaccag cacgatcatc
    51001 agcaggatga tagatccgag cagcatcgtg gcactgtagc gttgccagaa ctccatcggc
    51061 agacgcagcg taatgatcgc cagaataaac gccaaaatca gatagacacc atcacgcttc
    51121 gcgaagaaga acggatcgtt ggttaagcgt tgccctatgg gcattgacgc cgaggtcacc
    51181 atgataaagc caatcgccgc gaggccgaag gtcagccaca gtaaggtgcg atcgtacatg
    51241 atcaggctgt cggtatcttt ttcccgcgag cccatcaccc agccctttag cgccgtggag
    51301 atccagacca agatactgaa tcctggcagg cgcggcattt tcaggcgagg gagagataaa
    51361 cgcatcaacc taactccttc gccagacggg caaactcatt gcctcgttgt tcaaagttct
    51421 tgaactgatc aaggctggca caggccgggg agagcagaac catatcgccc ggctgaacac
    51481 gcggagccag caagcgcata gcctgttcca tagtttcggt ttgttctgcc acttccgggc
    51541 gtagcgccgc cagctgcgcg ccgtcacgac cgaaacaata cagacgtacg ttatcgccat
    51601 tcaggtaacg cgccagtggg ctaaagtccg ccgatttacc atcgccgccc agcaacaaat
    51661 gcagcgtgcc gtctacgtgc aggccattca gcgccgcttc cgtactgccg acgttggtcg
    51721 cttttgaatc gttaatccag cgcacaccgt tatgctccag caccacttca aagcgatgcg
    51781 gcagaccggt gaaggtggtt aacgctttca ggctgctggc gcgcggtaac cctgcggcat
    51841 ctgccagcgc cagcgccgcc agcgcattgg tgtagttatg ctgcccggaa agtttcatct
    51901 ctttcacatt cagcactttc tcgcctttaa cccgcagcca ggtttcgccc tgctgatgat
    51961 tcaggtgata gtcacccatg ttgacgccaa agctgacgca gcgttcatcc gcaccgcgaa
    52021 tcggcattgt taaggcatca tcagcattaa ccacgcaaac tttcgcgttt tcgtaaatgc
    52081 gcagttttgc tgcacgatac tgttgtaaac caaacgggta gcgatccata tgatcttcag
    52141 tcacgttcag aatggtcgct gctaccgcct gtaagctgga ggtggtttcc agctggaagc
    52201 tcgacagttc cagcacgtac agttcacact catcatccag tagcatcaac gcaggcaggc
    52261 caatattgcc acccacacca acgttaaccc ccgccgcttt cgccatttca cccactagcg
    52321 tggtgaccgt gcttttgccg ttagaaccgg taatcgccac aatcggtgct tgtgcttcgc
    52381 gacaaaacag ctcgatatcg ccaacgattt cgattccggc atcagcggca gcgcttaagg
    52441 atggatgcgc cagtgcaata ccgggactgg cgacaatcag atctgccgcc atcaaccatt
    52501 catcattcag actgcccgtg tggcgttcta cggcttcggg taatttatcc aggctaggcg
    52561 gtgtcatacg cgtatccata acgcgcggcg tcacaccgcg agcgaggaaa aagtccacgc
    52621 aggaaagccc ggtgaggccc aggcc
//
LOCUS       DADMGC010000014        47518 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.014, whole genome shotgun sequence.
ACCESSION   DADMGC010000014 DADMGC010000000
VERSION     DADMGC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.014"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(164..955)
                     /gene="cpoB"
                     /locus_tag="JNZ73_000820"
     CDS             complement(164..955)
                     /gene="cpoB"
                     /locus_tag="JNZ73_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HAY8731205.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(965..1486)
                     /gene="pal"
                     /locus_tag="JNZ73_000821"
     CDS             complement(965..1486)
                     /gene="pal"
                     /locus_tag="JNZ73_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HAY8731206.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1521..2813)
                     /gene="tolB"
                     /locus_tag="JNZ73_000822"
     CDS             complement(1521..2813)
                     /gene="tolB"
                     /locus_tag="JNZ73_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HAY8731207.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(2946..4202)
                     /gene="tolA"
                     /locus_tag="JNZ73_000823"
     CDS             complement(2946..4202)
                     /gene="tolA"
                     /locus_tag="JNZ73_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HAY8731208.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAK
                     AAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADI
                     NNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAA
                     KLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4267..4695)
                     /gene="tolR"
                     /locus_tag="JNZ73_000824"
     CDS             complement(4267..4695)
                     /gene="tolR"
                     /locus_tag="JNZ73_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HAY8731209.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4699..5391)
                     /gene="tolQ"
                     /locus_tag="JNZ73_000825"
     CDS             complement(4699..5391)
                     /gene="tolQ"
                     /locus_tag="JNZ73_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HAY8731210.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5388..5792)
                     /gene="ybgC"
                     /locus_tag="JNZ73_000826"
     CDS             complement(5388..5792)
                     /gene="ybgC"
                     /locus_tag="JNZ73_000826"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAY8731211.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQHIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(5942..6235)
                     /gene="ybgE"
                     /locus_tag="JNZ73_000827"
     CDS             complement(5942..6235)
                     /gene="ybgE"
                     /locus_tag="JNZ73_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HAY8731212.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6235..6348)
                     /gene="cydX"
                     /locus_tag="JNZ73_000828"
     CDS             complement(6235..6348)
                     /gene="cydX"
                     /locus_tag="JNZ73_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HAY8731213.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6363..7502)
                     /gene="cydB"
                     /locus_tag="JNZ73_000829"
     CDS             complement(6363..7502)
                     /gene="cydB"
                     /locus_tag="JNZ73_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY8731214.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTGGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7518..9086)
                     /gene="cydA"
                     /locus_tag="JNZ73_000830"
     CDS             complement(7518..9086)
                     /gene="cydA"
                     /locus_tag="JNZ73_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HAY8731215.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILIANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(9935..12568)
                     /gene="mngB"
                     /locus_tag="JNZ73_000831"
     CDS             complement(9935..12568)
                     /gene="mngB"
                     /locus_tag="JNZ73_000831"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HAY8731216.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGFRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKIWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVGVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(12586..14562)
                     /gene="mngA"
                     /locus_tag="JNZ73_000832"
     CDS             complement(12586..14562)
                     /gene="mngA"
                     /locus_tag="JNZ73_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HAY8731217.1"
                     /translation="MVLFYRAHWRDYKNDQVRIMMNLTTLTHRDALCLNARFTSREEA
                     IHALTQRLAALGKISSTEQFLKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVAT
                     LSEPLQWESVDGPEAIDLVVLLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATT
                     PDELLSALDDKGGTQPSASFSNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVY
                     VEKQGANGIEGRLTADQLNSATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEAL
                     IQQSLTLERGDETRTVQQDTQPVKSVKTELKQALLSGISFSVPLIVAGGTVLAVAVLL
                     SQIFGLQDLFNEENSWLWMYRKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGL
                     AANMIGSGFLGAVVGGLIAGYLIRWMKNHLRLSSKFNGFLTFYLYLVLGTLGAGSLML
                     FVVGEPVAWINNSLTAWLNGLSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMA
                     NGVYGPYAIFASVKMVSAFTVTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPM
                     AIEDPLRVIGSFVLGSMVTGAIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWF
                     GAALVGAAISTAILLIWRRHAVKHGNYLTDGVMQ"
     gene            14672..15394
                     /gene="mngR"
                     /locus_tag="JNZ73_000833"
     CDS             14672..15394
                     /gene="mngR"
                     /locus_tag="JNZ73_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HAY8731218.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15498..16367)
                     /gene="sucD"
                     /locus_tag="JNZ73_000834"
     CDS             complement(15498..16367)
                     /gene="sucD"
                     /locus_tag="JNZ73_000834"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAY8731219.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16367..17533)
                     /gene="sucC"
                     /locus_tag="JNZ73_000835"
     CDS             complement(16367..17533)
                     /gene="sucC"
                     /locus_tag="JNZ73_000835"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAY8731220.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17627..18844)
                     /gene="odhB"
                     /locus_tag="JNZ73_000836"
     CDS             complement(17627..18844)
                     /gene="odhB"
                     /locus_tag="JNZ73_000836"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAY8731221.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(18859..21660)
                     /gene="sucA"
                     /locus_tag="JNZ73_000837"
     CDS             complement(18859..21660)
                     /gene="sucA"
                     /locus_tag="JNZ73_000837"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAY8731222.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(21961..22677)
                     /locus_tag="JNZ73_000838"
     CDS             complement(21961..22677)
                     /locus_tag="JNZ73_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HAY8731223.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(22693..24459)
                     /gene="sdhA"
                     /locus_tag="JNZ73_000839"
     CDS             complement(22693..24459)
                     /gene="sdhA"
                     /locus_tag="JNZ73_000839"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HAY8731224.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24459..24806)
                     /gene="sdhD"
                     /locus_tag="JNZ73_000840"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24459..24806)
                     /gene="sdhD"
                     /locus_tag="JNZ73_000840"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HAY8731225.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(24800..25204)
                     /gene="sdhC"
                     /locus_tag="JNZ73_000841"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(24800..25204)
                     /gene="sdhC"
                     /locus_tag="JNZ73_000841"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY8731226.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25585..25692
                     /locus_tag="JNZ73_000842"
     CDS             25585..25692
                     /locus_tag="JNZ73_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731227.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            25898..27181
                     /gene="gltA"
                     /locus_tag="JNZ73_000843"
     CDS             25898..27181
                     /gene="gltA"
                     /locus_tag="JNZ73_000843"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HAY8731228.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            27572..28138
                     /locus_tag="JNZ73_000844"
     CDS             27572..28138
                     /locus_tag="JNZ73_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY8731229.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATCVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28189..30648
                     /locus_tag="JNZ73_000845"
     CDS             28189..30648
                     /locus_tag="JNZ73_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY8731230.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALALEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWYSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGQVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            30663..31394
                     /locus_tag="JNZ73_000846"
     CDS             30663..31394
                     /locus_tag="JNZ73_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8731231.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31391..32453
                     /locus_tag="JNZ73_000847"
                     /pseudo
     CDS             31391..32453
                     /locus_tag="JNZ73_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            32560..33651
                     /locus_tag="JNZ73_000848"
     CDS             32560..33651
                     /locus_tag="JNZ73_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAY8731232.1"
                     /translation="MLYRYTKEKIMRERRMPVLQWGMLCVLSLLLSIGFLAVHLPAAL
                     LLGPMIAGIIFSMRGITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAI
                     LLVTLLSSAIVGWLLVRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYL
                     RVLFVAGAAVLVTRMMLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLP
                     SGTMLIPMLAGAVLQSGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQ
                     ILLSIFALLAICAGMAWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMA
                     MQTLRLFSILLTGPAIARFISTYAPKRSA"
     gene            complement(33648..34439)
                     /gene="nei"
                     /locus_tag="JNZ73_000849"
     CDS             complement(33648..34439)
                     /gene="nei"
                     /locus_tag="JNZ73_000849"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HAY8731233.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34475..35209)
                     /gene="pxpA"
                     /locus_tag="JNZ73_000850"
     CDS             complement(34475..35209)
                     /gene="pxpA"
                     /locus_tag="JNZ73_000850"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HAY8731234.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(35199..36131)
                     /gene="pxpC"
                     /locus_tag="JNZ73_000851"
     CDS             complement(35199..36131)
                     /gene="pxpC"
                     /locus_tag="JNZ73_000851"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HAY8731235.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(36125..36781)
                     /gene="pxpB"
                     /locus_tag="JNZ73_000852"
     CDS             complement(36125..36781)
                     /gene="pxpB"
                     /locus_tag="JNZ73_000852"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAY8731236.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQQHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(36804..37547)
                     /gene="ybgI"
                     /locus_tag="JNZ73_000853"
     CDS             complement(36804..37547)
                     /gene="ybgI"
                     /locus_tag="JNZ73_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HAY8731237.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            37818..39299
                     /gene="dtpD"
                     /locus_tag="JNZ73_000854"
     CDS             37818..39299
                     /gene="dtpD"
                     /locus_tag="JNZ73_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HAY8731238.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTLF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(39449..40867)
                     /gene="phrB"
                     /locus_tag="JNZ73_000855"
     CDS             complement(39449..40867)
                     /gene="phrB"
                     /locus_tag="JNZ73_000855"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HAY8731239.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYETARKGK"
     gene            complement(40864..41373)
                     /locus_tag="JNZ73_000856"
     CDS             complement(40864..41373)
                     /locus_tag="JNZ73_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HAY8731240.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            41502..41819
                     /locus_tag="JNZ73_000857"
     CDS             41502..41819
                     /locus_tag="JNZ73_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HAY8731241.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSLHLK
                     GDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELREELYQVKQVVKGMRDGMFSVL
                     NES"
     gene            complement(41874..42254)
                     /locus_tag="JNZ73_000858"
     CDS             complement(41874..42254)
                     /locus_tag="JNZ73_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731242.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     EEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYTL"
     gene            complement(42257..46921)
                     /locus_tag="JNZ73_000859"
     CDS             complement(42257..46921)
                     /locus_tag="JNZ73_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY8731243.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGGTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNTWRCVYDERGNLLAVTDPLQQNTRYQYDHHGQVVQ
                     ITDARGGNKYLQWNEDGQLMRHTDCSGSQTAWFYDERTRLIRMTDAQSHSTRYGYDDS
                     GHLTEVILADGRTVNYQSDAAGRLVKYTSPMGRITRWQRDGQGRVRSRTDAMGRRTAF
                     GYDAYGRLVTLTNENGERYWFRHDVLDRLAEQINPDGCCQSYRYNALNAVTEVVFTGD
                     RGGEIRHRLARDAAGRLTAKETADGRTEYVHDAADQLLEIRRQRHETDAPEVIRFSYD
                     RLGRMLSEETAQGILTHQYDELGNRTATTFPDGRTQRHLYYGSGHLQQINLDREVISE
                     FTRDHLHREVLRSQGRLSTRQLYDPGGRLKRRETYSGMRGVVPETFTDRQYSYSGEDE
                     LLKTRHSRRGETDYFYDPTGSITACRSEDEGYLASWQYDAAGNLLGRRAGERATAENS
                     VVPFNRLMSYRGVHYRYDEFGRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYD
                     ALGRRTEKQHISPDGKPYNRTKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVD
                     KAGKEGPNRILYFHTDVNGAPEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRY
                     QGQYLDRETGLHYNLHRYYDPDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTP
                     CAVSNQKANRLLDSSETKVTVRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYL
                     TGNKTKAGSYHWDDIKDPSVTKPPYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPR
                     ET"
     gene            complement(46940..47161)
                     /locus_tag="JNZ73_000860"
     CDS             complement(46940..47161)
                     /locus_tag="JNZ73_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1795 domain-containing protein"
                     /protein_id="HAY8731244.1"
                     /translation="MLSQRGGLEIQSQFLRGNEQVYQCQVAFVLPGERVMMAFTYART
                     TPLTPADMTRWAEIKKNLRFRMRQEVRTN"
BASE COUNT        11292 a        13037 c        11529 g        11660 t
ORIGIN      
        1 ctttttcgtt gctttcggtt tgtttgatat cccgtggcaa cggcggcata tattactgat
       61 ttcagacttg agcgcaacaa aaatttcgat gcagatcact caactgctta tgattcgcac
      121 gacacgacca gaaataatgc gacttctggt cgtgttttat gcattacatc gcgttcagac
      181 gtttttgtgc ctgtttagcg ccatcagtac cagggtattt actgataacc tgctggtata
      241 cggctttcgc ttttgcggtg tcacctttgt cctgcatgat gacgccgact ttaaacatcg
      301 catcagcagc ctttggtgac ttcggatagt ttttcactac cgaagcaaaa tagtacgccg
      361 catcatcttt tttacccttg ttgtagttta actgacccag ccaataattg gcgtttggca
      421 ggtaagttga atcagggtaa tttttgatga aattctgaaa tgccaccatt gcgtcatcct
      481 ggcgggattt atcctgcacc agcgcaatag ctgcattgta atccgtgttt gcatcgccgc
      541 ttttcaccgg cgcgccagca ttcgcagtac cagcatcagc tgtcggcgtc gttgatgccg
      601 ccgcaccgct ttgatcgctg ctggttgatt gcgccgctgc accaccgctg ctgaggctgt
      661 cgatctgcaa caggatctgc ttttgccgct ccacgacctg attcagttga tactgatttt
      721 cctgaatctg accacgcagg gaatcaatat cggattgatt atcagaaagt tgttgctgga
      781 gttgggttaa aagctggctg tgagcattag aaatacgctc aagttgagtg acgcggtctt
      841 cgaccgagcc tgagccgaca ctactgattg gtgcctgagc aaaagcggcc cagggggccg
      901 ctataccaac cagtaacgac agactcaata gttgatgtct gaagttactg ctcatgcaat
      961 tctcttagta aaccagtacc gcacgacggt ttttggagta tgctgcttcg tcatgaccca
     1021 gtactgcagg tttttcttta ccgtaagaaa cgatggagat ctggtctgca gaaacgcctt
     1081 taccctgcag gtacatctta acggcgttcg cacgacgttc acccagggag atgttgtatt
     1141 ccggagtacc acgttcgtcc gcgtgacctt ctacggtgac tttgtaagac gggttgctac
     1201 gcaggaagtt tgcatgtgca tccagcattt gagcgaagtc agaacggata tcgtacttgt
     1261 ccagatcgaa gtaaacgatg ttgttctgct gcagctgttg catttgcaga cgagcctgct
     1321 cttcggaaga catgttgccg ttgccgccgt tcgcatccat accagtgcca gcacccagca
     1381 tgccttcgct gccgtcattg ctggcgttct tgttggaaga acatgccgca attgccataa
     1441 caggcagagc aatcatcagc cctttcagca ctttgttcag ttgcatttca atgattcctt
     1501 tactattcaa ttaattatta tcacagatac ggcgaccagg cagggaattt gacctgtcca
     1561 tcagttgccg gaagacgcgc tttgaaacgc ccatctgtag aaaccaaatt cagcacggat
     1621 cccatcccct gagaagagct gtagattacc atagtgccgt taggtgccag acttggcgtt
     1681 tcatccagga acgtggacga cagaacttgt acgcctcccg ttgccagatc ttgtttggca
     1741 atgtgctgct gcccaccatt ggagctgacc attaccataa atttaccgtc gctgctgaca
     1801 tccgcatcct ggttctgcga accttcccag gtaatacgtt gtggcgcacc gccgttgata
     1861 ttcactttat aaacctgcgg acgaccggcc tggtcagaag taaatgccag gttctggcta
     1921 tccgggaacc aggtcggttc ggtattgtta ctgcgaccat cagtcacctg gcggatctga
     1981 ccagaagcca aatccattac gtacaggttc agactaccgg ttttcgacaa ggcgaatgcc
     2041 agtttgctgc cgtctggcga gaatgcaggt gcaccgttgt gacgcgggaa tgaagccacc
     2101 tgacgtacag cgccatttgc cagcgtctga ataaccagcg cggaacgacc gctttcgaag
     2161 gtcacataag ccagtttaga accgtctggt gaccacgccg gagacatcag cggctgcggt
     2221 gaacggtgaa cgacaaactg gttgtaaccg tcatagtcag atacgcgcag ttcatacggg
     2281 aactgaccgc cgttggtctg aacaacgtag gcaatacggg tacggaacgc acctttaatg
     2341 ccggtcagtt tttcaaacac ttcatcactg gcggtatgac cagcataacg cagccactgc
     2401 ttgttcactt tgtacgagtt ctgagcaagt acagtacccg gtgcgccgcc agtgtcaaca
     2461 agttgataag caacattgta agaaccatcc ggattcggag tgacctgacc gacaactaca
     2521 gcgtcaatgc ccagtgcgga ccatgcagct ggttgtactt cctgcgcact acccggctgc
     2581 tgtggcagac gagcgcgatc taacggatta aatttaccgc tgttacgcaa gtcagcagca
     2641 acgatgccgc caatatcttc aggtgccgca ccaggccccg cccactggaa aggaacaaca
     2701 ccaataggac gaccggaatc tacaccgctg tcgatcacaa tgcggacttc agcatgcaga
     2761 actgatgccc acagtatgag aaaaccaaat gctactcgta atgcctgctt catcatatct
     2821 cccttatctg gaccgatggc ccacgataat ttagcagaat gttaacaaac taaaatacac
     2881 aaaactacca gaaccccgtg gcaaccggtg cctgatgttg accgtccgaa cagtcaacat
     2941 cgcgattacg gtttgaagtc caatggcgcg tttttgaaca cttcatatac tgcctggctt
     3001 ggtggtttcg ggatcttcgc aagtttagct gctgccaacg cagcctgaca aagtgcggga
     3061 tcgccacctt caggtttgat atccagtaac ataccatcgg gtgccagttt tatgcgcagc
     3121 gtacaggttt tgcctgcata ggacgatgcg tcatagaact tactttcgat agcagattta
     3181 atctgcccgg catagttatt gatatcggcc cctgatgcgc cattgttttt agtattacca
     3241 ctcccggcag gcgaagcatt gttccctttc gcccctcccc ccgttttcgg tgcattctta
     3301 ccagagctta gctcaccgaa aatatcatct gcctctgcgg cagcttttgc tgcagcggct
     3361 ttttcagctg ctgcttttgc cgctgccgct tttttgtctg cggcggcttt ttctgccgct
     3421 gctttcttat cagctgctgc cttatcagca gccgcttttt ctgctgcttt tttgtcggct
     3481 gcagctttct ctgctgccgc tttcttatca gctgcagcct tatcagctgc agccttttca
     3541 gcagccgctt tcttctcagc ttctgccttg gctttttcag cagcttcagt tgccgctttc
     3601 tttcttgctt cagctgcagc tgcttctgcc gcttccgctt tcttcttcag tgccgcagcg
     3661 gctacctcgg cttttttctg cgcttcggct gcggctttgg cggcttctgc ttctgctttt
     3721 ttcttcgcgt ctgcagccgc tttcttcgca gcttcttccg cagctttagc atctgccgcc
     3781 gctttcgctg ccgccacttc agcttgcttc tgctttaact cggcctgttt tgcggcttct
     3841 tcagcctgct ttttctgctc ctgagccgct aaccgctctt tctcaagttg cttcaggcgt
     3901 tcctgttcag ccgcttgttt ctcacgcagt tcttcagcag cctgctgttc cttcatcttg
     3961 cgctgttcat cagaacgctt cgcgcttgat tcctggcttt gcatgcgttt gtactgctca
     4021 actaccgcac ctgaatcaac catgacagcg tcgatggacg aaccaccgcc gcctccggct
     4081 gaagcttcta tattctcatc gaacgaactc cagatcagcg ccgcaaataa gatgacatgc
     4141 agcactgctg aaataattat cgcccgcttg agcttgtcgt tttgttcggt tgcctttgac
     4201 actctcggtt tccaaaaact gttcgcctgt tacccgctct ctttcaagca agggaaacgc
     4261 agatgtttag ataggctgcg tcattaaacc aaccgatttc acacccgcac tatgtaacaa
     4321 gttcagtgct ttaattattt catcgtaagg cacatctttt gcgccaccga tcagaaagac
     4381 cgttttcggg ttggccttga aacggctgga cacttccgcc accacctgct ctggtggtaa
     4441 acgctccagg cgatctttct caaccaccac ggtgtactga ccaataccag acacttcaac
     4501 aatcactggc ggattatcgt tactgctcac cgcctgtgat tcagtagcgt ctggcagatc
     4561 gacctccacg ctctgggtga tgatgggcgc tgtcgccata aagatcagca acagcaccag
     4621 cagtacgtcc agcaacggta caatgttgat ttcggacttg agatcgcgac gacctcgtcc
     4681 acgcgctctg gccatggctt accccttgtt gctctcgcta acggtaaacg cctggcggtg
     4741 cagaatcgcg gtaaactctt ccataaagtt gtcgtaattc agttccagtt tgtttacgcg
     4801 ctggttgaga cggttataag ccattaccgc cggaattgcg gcgaacagac caattgccgt
     4861 cgcaatcaac gcttctgcga taccgggcgc aaccatttgc agtgttgctt gttttaccgc
     4921 cccgagggcg ataaaggcgt gcatgatccc ccagaccgta ccaaacagac caatatacgg
     4981 gctgatggag ccaaccgtac cgaggaacgg aatgtgcgtt tccagatttt caagttcacg
     5041 gttcatggag atacgcatag cacgcgacgc cccttccact acggcttccg gcgcatggct
     5101 attggcacga tgcaggcgca caaactcttt gaacccgctg taaaagattt gttccgaacc
     5161 agtcagatta tcccgtttcc cctgactctc ttgatagagg cgagagagtt cgattccaga
     5221 ccagaattta tcttcaaacg cttcggcttc acgcgccgct gcgttaagaa tacgggttcg
     5281 ctggataata atggcccagg atgcgattga aaaaccaatc aaaatcaaca tgataagttt
     5341 aaccagaagg ctagccttca ggaacaaatc aaggatattc atgtcagtca ctgcttaaac
     5401 tccgcgacaa tagacttggg aagcgcacga ggcttcattt tgagtgggtc aacgcaaaca
     5461 accagaacct ctgcttcatt cagcaaagta ttctcggcgt tgacaatatg ttgcgtgaaa
     5521 accaaagagg tgccacgcat tgatgttatt tcagtctgta tttcgagcat atcgtcgagc
     5581 cgcgcaggtg cataatattc caccgtcatt ttacgtacca caaaggcaac gcgttcagcc
     5641 atcagcgcct gctgactgaa gtgatgatga cgcagcatct ctgtgcgtgc tctttcataa
     5701 aaagcgacgt aactggcgtg gtacaccaca ccaccggcat cggtatcttc atagtagacg
     5761 cgaaccggcc atcgaaacag cgttgtattc actttacatc ccggtaatgc aacaaaagtt
     5821 agagctttta aacgctgcta ctatacgcgc gcgattgagg tttgggaatg agggcaagtt
     5881 aagggagcgt aaaaatttat gggcctagag cggcccataa ttttttaaag agaattctga
     5941 tttaaaagaa gaagaaaatc agcccgacaa tgagaacaat atcggcaagc aatgggcaaa
     6001 aaatcccttg ccaaagaacc ttctgcggac gaaagcccac gccgtgaatc acaccagcac
     6061 agacggccca catcagcaat aaaccatgcc agatttccag ttcactggtc ttcgcggcaa
     6121 aacgagatgg gtcccaaaac atacatcctg ctaacagaag cgccatcacg aaggaaagcg
     6181 cccgcagggg gcgcttgtcc attaccgcat acaaggtcgc gataatctta ctcatcagat
     6241 gtcttcttga ccggctttgc ctgattcgac gtgttcaagc gccagcgcgg tgattacccc
     6301 aaacgaacag gcaagaagcg ttcccagaat ccatgcgaaa taccacattt ttagctcctt
     6361 acttagtaca gagagtgggt gttacgttca atatcttctt tggtgatacg accgaacatt
     6421 ttccagtaac accaggcggt gtagagcaga atgatcggta ccagaaccac cgcaacccag
     6481 gtcatgacgt taagcgtcag ctggctggaa gttgcatccc acattgtcag acttgcgttc
     6541 atcatggtgc tggacggcat cacaaacggg aacattgcga taccggctgt caggatgatg
     6601 caggccagcg tcagggagga gaacacaaac gcccacgcgg ctttatccat acgtgcagtc
     6661 aggatggtca gcagcggcag aaccacaccc agtgccggaa tagcccacag aattggcgtg
     6721 ttgttgaagt taaccagcca tgcgccagct tcacgaacca cttctttatt cagtgggtta
     6781 gaggctgcgt aatggtccat tgtcgatttc acgacataac catcgatacc gtacatcacc
     6841 catacgccag ccagtgcgaa acagaccagt gtcaccagcg cagccacctg agccgttgca
     6901 cgggtacgca ggtgcagttc gcccccggta cgcatttgca gataggttgc gccctgagta
     6961 atgatcatcc ctacgctcac cacgcctgcc agcaggccga acgggttaag caactggaag
     7021 aagttaccgg tgtagtacag acgcagatat tcatcaacgt tgaacggtac gccctgcaac
     7081 aggttaccga acgctacacc aattaccagc ggcggaacga agctaccaat gaagatgccc
     7141 cagtcccaca tgttacgcca gcgggtttct tcaatcttgg agcggtagtc aaaaccgacc
     7201 ggacggaaga acaaagacgc cagcacgagg atcatcgcca catagaagcc ggagaacgca
     7261 gcggcataga ccatcggcca ggcagcaaag agtgcgccgc ccgcggtgat cagccaaacc
     7321 tggttaccgt cccagtgtgg tgcaatggag ttaatcataa ttcgacgctc ggtgtcgtta
     7381 cgaccgagga aacgggtgag catgcccacc cccatgtcga aaccgtcagt gactgcaaaa
     7441 ccaatcagca gaacgccaac cagcagccac cagataaaac gcaatacttc ataatcgatc
     7501 atttgacgac tcctgtctta gcgtgccggc tgagtagtcg tggaagactg ctcaaagtga
     7561 tagcgaccgg ttttcaggct gcttgggccg aggcgtgcaa acttgaacat taagaacaat
     7621 tctgccacca ggaacagggt atacaggccg caaatcagca ccattgagaa gatgagatcg
     7681 cctgcggtca gtgacgaatt cgccacagct gtcggcagca cttcaccgat agcccacggt
     7741 tggcggccat attcagccac gaaccagccc gcttctacag caatccacgg cagcggaata
     7801 ccgtacagcg cggcgcgcag aagccatttt ttctcgccaa tgcggttgcg gatgacactc
     7861 cagaaagaga gcgcgatgat tgccagcagc aggaagccac acgccaccat gatacggaac
     7921 gcaaagtaca gcggcgctac acgcgggatg gagtctttgg ttgcctgttg aatctgcgct
     7981 tcagtcgcat cagccacgtt tggcgtatag cgtttcagca gcagaccgta accgaggtct
     8041 ttcttcatgc tattgaactg gtcacgaacc gcctggtcgg tagaaccaga acgcagttgt
     8101 tcgagcagag agtacgcctt catcccgtta cgaatgcgtt cttcatgctg caccatcagc
     8161 tctttcaggc cgataaccgg ggtatccacg gaacgcgttg caatgatgcc cagtgcgtaa
     8221 gggatctgaa tcgcaaattt gttcgtctcc tcttcctgat caggaatgcc gaacaaagta
     8281 aaggcagcag gcgcaggttg cgtttcccac tcggcttcaa tagcagccag tttggttttc
     8341 tgcacgtcgc ccatttcgta gccggattca tcacccagaa caataacaga cagaacagca
     8401 gccataccga agctggcagc gatagcaaag gagcgtttag cgaaggcgaa gtcacgacct
     8461 ttcagcatat accatgcgct gataccgagg atgaacatcg cgccagtcac ataaccagac
     8521 gctacagtgt gaacgaattt cacctgagca accgggttaa gcaccagctc ggagaagctc
     8581 accatctcca tacgcatagt ttcaaagttg aaatcggacg cgattgggtt ttgcatccag
     8641 ccgttcgcaa tcagaatcca cagtgcggac aggtttgaac cgagcgccac cagccaggtg
     8701 acacacatat gctgaacttt acccagacga tcccaaccga agaagaacag acctacaaag
     8761 gtggattcga ggaagaaggc catcagacct tcgattgcca gcggcgcacc gaagatatcc
     8821 cctacatagt gggaatagta agaccagtta gtcccgaact ggaactccat ggtcagaccg
     8881 gtagccacac ccagagcgaa gttgatacca aacaacttgc cccagaactt ggtcatatct
     8941 ttataaatct gtttgccgga gaggacgtag accgtttcca taatggccag gaggaacgcc
     9001 ataccgagcg tcagtggcac aaaaaggaag tggtacatcg cggtcaaggc aaactgtaag
     9061 cgcgacagtt cgactatatc taacatcatg actccttgct catcgcatga agactccgag
     9121 agtgaacccc gttagaaagg gtcacacgca tgccccaata gaaatttgtt tgaacacttt
     9181 catcatacca tgtgaatgca cacaaaaacg gcgtaaagaa aggttgcaaa catgttaata
     9241 aaaactcaaa ttgatcccac gtatatatta cgccgcaaaa tccttacaat aaacaggttt
     9301 ttgttgaagc aaatttgcat ttttcgacgg tgatcaattt atagctaaat tgccgccttt
     9361 cagccaattt gatcgagaac aatttatctc tttttgatgc ccatttccaa gacttataca
     9421 ttgataaata tcaatttcta caaattatgt taatcatgtt tcaacaaggt aactttagtg
     9481 acatttatgt ttaaaatgtg tgaattataa gactgaaatt agtttcggtt ggcaattaaa
     9541 gataaaaaga gacgaagcaa aaacagcacg gttgacctta attctttttt tggtcactgg
     9601 ctaatacgac gcagagtttc aggattatta ataaaggcat aaatctcctc cccgccgata
     9661 ttaacattta attaacttat aattatatta taattaactc acccggggtt attattttgt
     9721 taaaaataaa gccacatata cgtagcttga gagtgaagat aaacgcaaaa ttacctgatg
     9781 cgctacgctt gccagtgtct acatcatctc tgcaatatat tgaatttgcc tgcttttgta
     9841 gaccgaataa agcgttcatg ccgtatccgg catgaacaaa gcgccctttg tcaacaatct
     9901 ggccgcgcat atgtggcttc ggtttgattg ctattcaggc aagccggtaa ctgaacgtcc
     9961 gtgactggcc gggtaaaaaa ggccccgata gatttgaacc ttgattttcc tcggtggtaa
    10021 tgtgttcatc catcatcgtt tctgagcaag aaatcacctc gcgactgaaa gcaacagtcg
    10081 catcacaggt tgctgattca gccggattaa acagccgtaa aatcacttct tgtcggtctt
    10141 cagctttctt aagtgcgctt atcaggcatc ccactggggg cattttcaac aaactataac
    10201 tttccggcac gttgaatccg gctttgttga gcttcatcgc atcccatggg attttgttgt
    10261 aacactgtac tggagtcagc catgctcgcg cctgctgagc tacaccagcg gcggttggcg
    10321 taccggtata actcaataaa cttaggcgac aagaaagcag accacgtagt tgtgagtccg
    10381 ggactggcat tttaattccc gaaggccgcc taggccttaa aagcagatct tctttcccca
    10441 gtaagcccac gccgcgcagc aacgtaatgg caaaggtttt cttctcttca ccgatgactt
    10501 caaattcacg tagcccttcg ctaaagacag ccatgccgtt acgcccttcc tgtaaggctg
    10561 catagttgag catattccat accggaaccg gcgcttcttt ccagccttct tgctgccagt
    10621 tgttcattgc actgtcgttc acggggcgcg ttagcgaacc aaactgggta tctgccagaa
    10681 cactatcggt gttaaaagat gtagggatca ggacgcgaag gcgatgatcg tcagcctggt
    10741 tatcaaggtt gatatccaca tcaatgcgcc tgttgttatg actaagagtg acaaccaact
    10801 cgacacctac tctgccagtg gattgtcttg cgctgcgttg ttgcagatta cgcggcaccg
    10861 ccatttcata gcggataaca gccctgctct gccaggcctc atgaataatc tcgcattgcg
    10921 gtttcgcatt cgccgaagta attacccact cttcttttgc gggtgaatag tcatattcat
    10981 caccatcatc agagctttct tcaatttgca acacccggtc atagcgcaca ccgctatctt
    11041 tatctaccag ttgtagcgtt ccatcctcat tgagcgcaat ttgccagaaa gcattttcca
    11101 gtatcccttc agcgtcactt tttgccgcaa tcacgttgcc aggctgattc gcttcgatat
    11161 aaagcgtgcg atagcccata gaagggacaa tctggttgat ctgtatatca aactccataa
    11221 agggatcgta attaccgtaa tgaactattt gccgatcgat taggcctgga tcgatctcac
    11281 gggcatggcg aataaaatac ggtacaggct gaccgcgatc gtctcgtaga ttaaactggc
    11341 tggcgcgcag ccgcacagtg gtgttgatca cttcttcccg cggccagggc atcaggttaa
    11401 acaggacgag tttgtcggcg tcgctctgcg gcatgttgtc ggcgatttta cgcatataga
    11461 aacgcagcag attatccgcc atgtcttcag ccagttcgaa gcgggcgacg atttcgcgat
    11521 gaactttgtc actacagcag cagccgatgc tgtcgtgggc atgatttttt aagatctctt
    11581 tccatatttt ctccagcaag ccgtggtggt attcaaaacc caacgtccag gccagcgttg
    11641 ccagcggttc cagcagatta acaatcttat tttcaatacg tgcgtgggca actttgatat
    11701 ccatgcgcgt ggaaccgatg gtgcgatgca cgcgcatata tttgccatca ataaattccc
    11761 ctttcagggt tgccagatta tctcgctgcg cttcgatctt ctcaaatacc tcttcaaagc
    11821 ggctcatcac aaactttcgt tgaggataaa tttcgcgcag tttctccatc acttcgaaga
    11881 tattttgctg caatggcatc tgatcatgcc cattcggcag caaaatctct ttggttacag
    11941 acgctttttc cagcacgtca aaataactgt cgaggcgttt gcggaatccg ttttcgtccg
    12001 caggtaagta cttaccgatg gcataaccca acggcatcac ctgcgccgtc acttcgctac
    12061 cgtcactgct ttgccacaaa aactcggttt tatccgtacc gtggcgctca gaacatccac
    12121 gccagaacat ggtgcaggta atgccaaatc cattgtagat atgcggcagt tgcccggaca
    12181 tgccaaagga atcgggtaaa taacctattt tcatcggctc gccaaacgcg aggcagtcac
    12241 gcattccgta catcagatta cggacgatgg attccgcaga aacaatcgtg gtatcggtct
    12301 gggtatacca ggggccgata atcaacttgc cggcttctac cagtttcttc acgcggtctt
    12361 tgttttccgg tttcaccgcg aaataatctt cgaggatcgc cgtttgcccg tcgagtacgt
    12421 aatatttgta ttcgttgtcc tgttccagtc ggcacaggat ctcttccata ttattgacca
    12481 gcagaatgcg tgactcttcg gtggtgaaat accactcccg atcccagtgc atatgcgggg
    12541 tgatgtgaac gcgagatacc gctttcatcg tcgtttcctt tttgtttatt gcattacgcc
    12601 atcagtcaga tagttgccat gcttaaccgc gtgacgccgc cagatcagga gaattgcagt
    12661 cgagattgca gcccccacca atgccgcacc aaaccagcca attgccgcca taacaccgcc
    12721 cgcgccatta tcatgaagta aaaagagcga gaaaatgcca gcaccgggtg tcgaaaggcc
    12781 gatattcatc gcaccgacaa tagcgcccgt taccatagag cccagcacaa acgaaccaat
    12841 aacccgcagc ggatcttcaa tcgccatcgg gatcgcccct tcggtaatac ctgccagccc
    12901 taacagccag gtggatttcc cggtctcaat ttcaaactct ttaaacaggc gcggtgcgag
    12961 catcgtggaa gcggttacgg taaatgccga aaccattttg acggaggcga aaatggcata
    13021 cgggccgtaa acgccgttcg ccattgcgcc caggcagaat gcataagcgg ctttattcac
    13081 tggccctcca aggtcaaagg aacacataaa accgagaatg gcacccagca acagcgcgtt
    13141 acttcctgac agaccgttca gccaggcggt aagcgagtta ttgatccagg cgacaggttc
    13201 ccccaccaca aacagcatca gactgcccgc tcccaatgta ccgagcacca ggtagagata
    13261 aaaagtcagg aagccattga atttactgct aagacgcaag tgatttttca tccagcgtat
    13321 caagtaaccg gctatcaatc cgccaacgac cgcgccgaga aacccggagc cgatcatgtt
    13381 ggcggcaagt ccagccgcaa agcctggcgc taacgccggt ttatctgcca gcgaataggc
    13441 ggtataggct gccagcaccg gtaccatcaa aattccgagc agcccgccgc ccagtttgcg
    13501 gtacatccac agccaggagt tttcttcatt aaacagatct tgtagcccga agatttgcga
    13561 cagtaatacc gcgaccgcca gcaccgtccc ccccgcgaca atcaacggta cggaaaaaga
    13621 gatcccgctc aacagtgcct gtttcagctc cgttttgacg cttttcaccg gttgcgtatc
    13681 ttgctgtacg gtacgcgtct catcgccacg ctcgagggta agcgattgtt ggatcaacgc
    13741 ttctgcatgg cgaatcggct cggcaacagg cacagaaagc gcaggaatgc cgttaaaacg
    13801 ctcactctcc ttgatggcga cttcagccgc aaaaatacag gcagttgcac tattgagttg
    13861 atccgccgtt aaacgccctt caataccgtt agcaccttgc ttttcaacgt agacattcac
    13921 gccgagtttg cgtccggctt tttccagata ctccgcagcc atgtaggtgt gagcaatacc
    13981 cgccgggcag gccgttacgc aaacgatagt tggcgcgttg gaaaaagagg cagaaggttg
    14041 cgtgcctccc ttgtcatcca gcgccgagag caattcatca ggcgtcgtcg ccgactgtat
    14101 acgcgcccga atctcatcat ccgccaggcg cgtggtcagc gctgtcagca gttgcatgtg
    14161 cgtagtaccc gcttcattgg ggggaatcgc cagcaacacc actaaatcaa tcgcttccgg
    14221 gccatccacg ctttcccact gaagcggctc gctgagtgtg gcgaccgcaa acgccgcttc
    14281 ttttaccgca gcagttttgc catgcggcac agccaaccct tcacctaagg cggtcgggcc
    14341 aaggctttca cggcgataca cttctttcag aaattgctca gtactggaaa ttttccccag
    14401 agcagcaaga cgttgagtca acgcgtgaat ggcctcttca cggctggtaa agcgcgcatt
    14461 cagacacaac gcatcgcggt gggtcagagt cgtcaggttc atcatgatcc tcacttgatc
    14521 gtttttatag tcgcgccagt gtgcccgata aaacaatacc attgcgtaat caatttcact
    14581 tgattgaaat tatttgcatt gtatttgtat tatttacagg atgaatttat cgggcataat
    14641 ggctaaattc gtattaatga gatgctaaat aatgggacac aaacccttat accgacagat
    14701 tgctgatcgc attcgtgagc aaattgcgcg tggcgagctt aaacctggcg atgcgttacc
    14761 taccgaatcg gcattacaaa cagagtttgg cgtcagccgg gttacggtgc gtcaggcttt
    14821 gcgccagtta gtcgagcaac agatcctcga aagcattcag ggcagcggga cttacgtcaa
    14881 agaagagcgg gtcaattacg atatttttca gttaaccagt tttgatgaaa aactgtcgga
    14941 ccgtcacgtc gatacacaca gtgaagttct gatatttgaa gtgattccgg ctgacgattt
    15001 tcttcagcaa cagctacaaa tcaccgtgca ggatcgtgtg tggcatgtga agcgcgtgcg
    15061 ctatcgcaag caaaagccaa tggcgctgga agaaacctgg atgccgcttg cgttgttccc
    15121 ggatctcacc tggcaggtca tggaaaattc gaaatatcac tttattgaag aagtgaagaa
    15181 gatggttatc gatcgtagcg aacaggaaat cattcctctg atgccaaccg aagagatgag
    15241 ccgtctgctt aatatcagcc agacaaagcc gattcttgaa aaagtatcgc gtggatattt
    15301 agttgatggt cgggtgtttg aatatagccg caacgctttt aataccgatg actataaatt
    15361 taccctgata gcgcagcgaa aatcatcgcg ataagcacaa aaaaggccat catacgatgg
    15421 ccaaccatgt cgaaaccgac agcccttcgc cattcctgta gagggaagcg gcgagggcta
    15481 tttcttatta cagatattta tttcagaaca gttttcagtg cttcaccgat atccgccagg
    15541 ctgcgaacgg ttttcacgcc tgcggcttcc agagcagcga atttctcatc cgcagtccct
    15601 ttcccaccgg caatgatcgc acccgcgtgg cccatacgtt tgcctttcgg cgcagtcaca
    15661 ccagcgatgt aacccacaac tggcttggta acatgctctt tgatgtacgc tgccgcttct
    15721 tcttcagcgc taccgccgat ctcaccgatc atcacgatcg cttcggtctg cggatctttt
    15781 tcgaacattt cgagaatgtc gataaagtta gagccaggga tcgggtcacc gccgataccg
    15841 acacaggtcg actgaccgaa accgtaatcc gtggtctgtt taaccgcttc ataggtcagt
    15901 gtaccggaac gggaaacgat acccacttta cccggtttgt gaatgtgacc cggctggata
    15961 ccgattttgc attcacccgg ggtgataacg cctgggcagt tcgggccgat catacgaacg
    16021 cctgcttcgt ccagtttcac tttcacggtc agcatatcca gcgtcgggat gccttcagtg
    16081 atggtgataa tcagtttgat gcctgcatcg atagcttcca gaatggagtc tttgcagaac
    16141 ggtgctggta cgtaaataac agacgcggaa gcgccagtag cagcaacagc ttcacgcacg
    16201 gtgttgaaca ccggcaggcc gaggtgggta gtgccgcctt tacctggagt tacgccacca
    16261 accattttag tgccgtatgc aatggcctgt tctgagtgga aagtcccctg gctaccggta
    16321 aagccctggc agataacctt ggtgttttta tcaattaaaa tggacattat ttcccctcca
    16381 ctgcggcaac aacctgctga gctgcatccg tcagaccttt tgctgcaata atattcaggc
    16441 cgctgtcagc cagtttcttc gcgccgagtt cggcgttgtt accttccaga cgtaccacga
    16501 ccggtacgtt aacacccact tccgctaccg cgccgatgat accgtcagcg atcaggtcgc
    16561 aacgaacgat accgccgaag atgttaacca gaacggcttt cactttgtcg tcagagagga
    16621 tgattttgaa cgcttcggtt acacgttctt tggttgcgcc gccgccaacg tccaggaagt
    16681 tagccggttc gccgccgtgc agtttaacta tgtccatcgt acccatcgcc agacctgcgc
    16741 cgttaaccat acaaccgatg ttaccgtcca gcgcaacgta gttcagttcc cactgtgcag
    16801 cctgtgcttc acgcggatct tcctgcgact ggtcacgcat ttcgcgcaga tcaggctggc
    16861 ggaacagggc gttgccatca gcgcccagtt tgccgtcgag gcaaatcaga tcgccctgtt
    16921 tggtgatgac cagcgggttg atttcgatca gcgccaggtc gcgctccagg aaaatggtcg
    16981 ccaggcccat gaagattttg gtgaactgct gaaccagttt accttccaga cccagtttga
    17041 acgccagctc gcgtccctga tacggcatcg ggccagtcag cggatcaagc gcaactttat
    17101 ggatcaggtg cggagtttct tccgccactt tttcgatttc cacgccgcct tcggtggagg
    17161 ccataaagac cacgcgacgg gaactacggt ctacaacggc accgagatac agctctttag
    17221 cgatatcggt cgccgcttca accagaatct ggttaaccgg ttggccattg gcatctgttt
    17281 gatacgttac cagacgcttg cccagccagt tttctgcaaa agcacggatg tcttctttgc
    17341 tgtttacaac tttcacaccg cccgctttac cgcggccacc agcgtgaacc tgacatttca
    17401 ctacccacgg accggcaccg atttttgaag cggcttcttc tgcttcgcgc ggagtagtac
    17461 aggcataacc caccggtgct ggtaagccat agcgggcaaa cagttgtttt gcctgatatt
    17521 catgtaagtt catgtgttct gtccatcctt cagtaatcgt tatcttttaa accgtagacc
    17581 gacaccctat ccacgtcggt ctacagtgca ggtgaagctt aaactactac acgtccagca
    17641 gcagacgcgt cggctcttcc agcaactctt tgatcgttac caggaagccc acggattcgc
    17701 gaccatcgat cagacggtga tcgtaggaca gcgccaggta catcatcggc aggatctcaa
    17761 cctgaccatt caccgccatc ggacgatctt tgatagcgtg catacccaga attgcgctct
    17821 gcggcgggtt gatgatcggc gtagacatca gggaaccgaa cacaccaccg ttggtgatgg
    17881 tgaagttacc accggtcaga tcttcaaccg tcagcttgcc atcacggcct ttgactgcca
    17941 gctctttgat tttcttctcg atgtctgcca tgccgagggt atcgacatca cgcagaaccg
    18001 gcgtcaccag gccgcgcggc gtagaaaccg ccatgctgac gtcgaaatag ttgtggtaaa
    18061 ccacgtcatc gccgtcgata gacgcgttca cttccgggta acgtttcagg gcttcaacca
    18121 ccgctttcac gtagaaggac ataaagccca gacggatgcc gtggcgtttt tcaaacgctt
    18181 caccgtactg cttacgcaga tccataatcg gcttcatgtt gacttcgttg aacgtggtca
    18241 gcatggcggt ggagtttttc gcttccagca gacgctctgc cacacgctta cgcaggcgag
    18301 tcatcgggac acgtttttca ctacgtgcag ccagagccgg ttgcgccgcc ggagcagccg
    18361 ctgccggagc agactctttc gccggggctt tcgccagatg tttttccaca tcttcacgag
    18421 tcagacgacc acccacaccg gtgcctttaa tggcgctggc gtcgagattg tgttcagcca
    18481 gcaggcgacg gatcgccggg cttaacgcat cgttgttttg ctcttccaga gacgcctgct
    18541 ggcgttgcgc cggagtggac gctttctctt cagatttggc gctggtttct ttaccggcgc
    18601 tgttgccttc acgcaggcga ccaaggatct gacgagacgt taccgttgta ccttcatctt
    18661 ccagaaccgc atccagaatg ccgtctgctg atgccggtac ttccagtacc actttgtcag
    18721 tttcgatttc taccagcact tcatcacgta cgactgcgtc gccgggtttt ttatgccagg
    18781 ttgcgacggt ggcatcggct acggattcag gcaggtcagg gaccagaata tctacgctac
    18841 tcattgtgta tcctttattt attcgacgtt cagcgcgtca ttaaccagat cttgttgctg
    18901 tttctggtga acggacatat accctaccgc cggagaggcg gaggccgggc ggcctgcata
    18961 acgcagagaa gccccaaacg gaatcacttc acggaaatga tgctggctgc agtaccatgc
    19021 gccctggttg agcggctctt cctggcacca gacaaaatcc ttgacgtgag caaactgctg
    19081 caacacttcc tgcatcgctt tatgcgggaa cgggtagagt tgctcgatac gcacaatggc
    19141 gacatcgtgt tgattgttct tacgacgctg ttccagcagg tcgtaataaa ccttaccaga
    19201 acacatcact acgcgcttca cgcccttcgg atcaagttcg tcgatttcac cgatggctgg
    19261 caggaaggtg ccgttcgcca gttcttcgag gctggaaacc gccagcggat gacgcagcag
    19321 ggatttcggc gacatcacga ccagcggacg acgcatcccg cgcagcgcct gacgacgcag
    19381 catgtggtaa acctgtgccg gggtagacgg tacgcaaacc tgcatgtttt gctcagcaca
    19441 aagttgcaga taacgttcca gacgcgcgga ggagtgctcc ggcccctgcc cttcgtaacc
    19501 gtgcggcagc aacatcacca gaccacacat ccggccccat ttctgctcgc cagaggagat
    19561 gaactggtcg ataaccacct gcgcaccgtt ggcgaagtca ccgaactgcg cttcccagat
    19621 ggtcagggtg cgtggttctg cggtggcata accatattca aacgccagca cggcttcttc
    19681 tgacagtacg gagtcccaga cacggaacgc gccctgaccg ttatggatgt gttgcagcgg
    19741 cgtgtaagtg gaaccgttag actggttgtg gatcaccgcg tggcggtgga agaaggtacc
    19801 gcgaccggag tcttcacccg acaggcgaac cggaatgcct tcatcaacca gcgtggcgta
    19861 agcgaggttt tccgcaccgc cccagtcgaa cagtttctca ccggctgcca tcgcctggcg
    19921 atcgccataa atcttggcaa cgcgagactg catttcaact gcttctggta ccgtactgat
    19981 acgtttagcc agttcctgca ggcgcttcat ctcaactttg ttcgggtact cttcgtccca
    20041 ttcgtggttg aggtacggcg accaggtgaa agagtgcatg ttcatcggac gccactctgc
    20101 cactacgcaa tcgccagcat ccagcgcatc gcggtacagg ttaaccatct cggtggcatc
    20161 ttccagcgtc gccacttttt cctgctccag cttgtcagcg tagattttgc gcggtgtcgg
    20221 atgttttttg attttctgat acatcagtgg ctgggttgcg ctcggctcgt cggcttcgtt
    20281 gtggccgtga cggcggtagc ataccaggtc gatgaagaca tcacgtttaa aggtgttacg
    20341 gaaatcgagc gccagacggg tcacaaaggc aacggcttcc ggatcatccg cgttaacgtg
    20401 gaaaatcggt gcctgaacca tcttaccgat atcagtacag tacggtgtag aacgggcatc
    20461 cagcgggtta gaggtggtga aaccaacctg gttgttgata acgatacgta ccgtaccgcc
    20521 aacttcataa ccacgcgctt tcgacatgtt cagagtttcc tgaaccacgc cctgcccggt
    20581 cactgcggcg tcaccgtgga tggtgattgg cagcactttg ttgctgctcg gctcatcaag
    20641 tctgtccaga cgggcacgaa cagagccgat aacgaccggg cttacaatct caaggtgaga
    20701 cgggttaaac gccagcgcca ggtgcaccag gccgccatcg gtctggaagt cagacgagaa
    20761 gcccatgtgg tatttcacgt cgcctgtgcc gaggtgttct ttatgtttac cggcgaactc
    20821 gtcgaacaag tcttgcggtt ttttacccag cacgttcacc agcacgttca gacgaccacg
    20881 gtgcgccatc ccgagaacca cttcgcgggt gccgctgttg ccagcgtggc ggatcatctc
    20941 tttaagcatc gggattaacg cgtcaccgcc ttccagcgag aagcgttttg cgccagggaa
    21001 ttttgcgccg aggtaacgct caaggccttc agcggcggtc agttcgctta agaagcgttt
    21061 tttctcttcg ctattgaaag tcgcgcgacc agactcgata cgctgttgga tccagcgttt
    21121 ttcttcggtg ctggtgatgt gcatatactc ggcaccaatc gggccgcagt aggtttgctt
    21181 gagggcggaa ataagctcgc cgagtttcat ggtttctttg ccgctggcaa atgaaccgac
    21241 gttgaaggtt tcctggaagt ctgcttcggt cagatcgtgg aaagacggat ccagatcggc
    21301 cactttatct tgctgccaca gtcccagcgg atctagattc gcatgctggt gaccacggaa
    21361 gcggtatgcg ttaatgagct gcaggacttt aacctgcttc acattggtgt cagggtcgga
    21421 gatcgttgaa gagtaacgtg aagcgtcttt cgccaggcgg cggaaatatt cacgcgtttg
    21481 agagtggaat tgatccggtt tgactcccgt accaggtaac tgctggaacg tcgaacgcca
    21541 gttagcgtca accgagtcag gatcggttaa gaagtcttca tagagctgtt ctatccagct
    21601 ctggtttgcg ccagagaggt aagaagagtc caaccaggct ttcaaagcgc tgttctgcat
    21661 cgtgatccct taagcatctt ttttatgctt acttcgccgt ggatactacc acgcacagtg
    21721 atgtgcgtgc cggggttcac ttgcgagctc ttgtctacgt agtggctcgc gaaggaacct
    21781 ttaaaaactg tcttagtgtc aggggcagtt tttaaagatt tcctgcattt ctttgttacg
    21841 cctgatgcgc tgcgcttatc aggcctgcgg gtaatgactt gtaggccgga taaggcgttc
    21901 acgccgcatc cggcactggt tgcctgatgc aacgcttgcg cgtcttatca ggcctacagt
    21961 ttacgcatta cgttgcaaca acatcgactt gatatggccg atggcgcgcg tcgggttcag
    22021 ccccttcgga catacactga cgcagttcat gatgctgtga cagcggaata cgctgaatgc
    22081 atcgctcaaa ccgtcgaggc ggctgtcagt ctcggtatca cggctatcga tcaggaaacg
    22141 atatgccgct aacaagcctg ccgggccgat aaacttatcg ggattccacc agaaagacgg
    22201 acaagaggtt gaacaacatg cgcagagaat acattcatac agcccgtcga gtttttcgcg
    22261 ctgctctggc atctgtaaat gctcgcgagc tggcggattt tgtccattat tcaacaggta
    22321 aggcttaatt ttctcatatt gcgcatagaa ttgtcccatg tctaccacca aatcgcggat
    22381 caccggtaaa cctggcagcg ggcgaatcac aatcttcttg cccggctggt tgagtgccga
    22441 aatcggggta atacaggcca gaccattctt gccgttcatg ttcagaccgt cggaaccgca
    22501 cacaccttca cggcaggagc ggcggaacga caggctggga tctttctctt tcagctggat
    22561 aagcgcatcc agcagcatca tgtcgcgacc ttcttccgct tccagggtgt aatcctgcat
    22621 acgcggagca tcatcaacat ccgggttata gcgataaatt gaaaactcga gtctcatttt
    22681 cctgtctccg cattagtaag tacgaatctt cggcgggaat gccgggcgca gtttcggttc
    22741 catgttgacg cttcggcgcg tcatggattc cgactcaggc agatacaggg agtggcacag
    22801 ccagttttca tcatcacgat ccgggaagtc gaagcggcta tgtgcgccac ggctttcggt
    22861 acggaagttg gcagaaacag ccgttgcata cgccgtttcc atcaggttat ccagttccag
    22921 gcactcaacg cgctgggtgt tgaactcgct ggaagtgtca tccagacggg catttttcag
    22981 acgctcgcgg atcactttca actgctcaag ccctttcgcc atcgcatcac cttcacggaa
    23041 gaccgagaag ttatgctgca tacattcttg cagcgcttta cggatcgcca ccggatcttc
    23101 accgttacga ttattgttcc accggttcag gcgatccaga gacgcttcaa catcagactc
    23161 gctggcatcg cgcagtgcgc cctgctcggc gatagactct tgcagatgca gacctgccgc
    23221 gcgaccaaag accaccaggt ccagcagcga gttgccgccc agacggttag cgccgtgtac
    23281 cgatacacaa gcgatttcac caacggcaaa cagtcccgga acaaccacat cttcgccttt
    23341 ctcattcaca gtcagcgcct gaccggtaac tttggtcgga ataccgccca tcatgtagtg
    23401 acaggttggg ataaccggaa tcggctcttt caccggatcg acgtgagcga aggtacggga
    23461 aagctccagg atacccggca gacgggattc gagaacttct ttacccaggt gatcgagttt
    23521 cagtttcgcg tgtggccccc acggaccatc acagccgcga ccttcacgga tttcaatcat
    23581 gatggaacgc gccaccacat cacggcccgc caggtctttg gcgttcggcg cataacgctc
    23641 cataaagcgt tcgccatgtt tgttcagcag ataaccgcct tcaccacggc aaccttcggt
    23701 gaccagtacg cccgcaccgg caatgccggt cgggtggaat tgccacattt ccatatcctg
    23761 caccggtacg cctgcgcgga tagccatgcc gacaccgtcg ccagtgttaa tgtgggcgtt
    23821 agtggtggac tgataaatac gccctgcccc gccagtcgcc agcacggtag cgcgggcttt
    23881 gaaataaacc acttcaccgg tttcgatgca cagtgcggta caacccacca ccgcgccatc
    23941 ctggtttttc accagatcca gcgcatacca ctcggagaaa atggtggtgt ggtttttcag
    24001 gttctgctga taaagcgtgt gcaacagtgc gtgaccggta cggtcagcag ccgccgcagt
    24061 gcgtgccgcc tgctcgccgc cgaagttttt cgactgaccg ccaaacggac gttgatagat
    24121 acgaccatca tcgagacgcg agaacggcag gcccatgtgt tcgagttcca gaatcgcttc
    24181 cggcccggtt ttacacatat attcaatcgc gtcctggtca ccgatatagt ccgacccttt
    24241 tacggtgtcg tacatatgcc attcccagtt atcttcatgg gtattaccca gcgcaacggt
    24301 aatgccgcct tgcgcagaaa cggtatggga acgggtcggg aagactttag agagcagcgc
    24361 acaggtctgg ccgctctggg aaatttgcag cgccgcgcgc atacctgcgc caccggcacc
    24421 aatcacaact gcatcaaatt ctctgactgg caatttcatc acacccccca caccacaacg
    24481 aatccataaa tcacgtaaac caccagtgca acgacaatca ccagttgcag catcaggcgc
    24541 aaggccagcg gtttaacgta gtcggtcaac acctgccaca tgccgatcca ggcatgaatc
    24601 aagatagaaa acagcgccag cagggtgaac actttggtga acgcagaggc gaagaaaccg
    24661 atccagactt catatgtcag ttcgccactg gtggcgaaaa aaccgaccat ataaatgatg
    24721 tagagcgtca ggacgatagc ggtagcgcga acgaggatga aatcatgtac gccattgcgt
    24781 cctaatgcgg aggcgttgct taccatacga ggactcctgc gagaagtgaa agcacgacag
    24841 taataacaaa ggagattttg gcggagcgtt tacccgcttc gaatgtttct tccagatagc
    24901 caaaatccat catcatgtgg cgaataccta cgacgacgtg atacgccaga gcggtaagga
    24961 tgccccacat gataaatttg acgaagaagc tgcccataat cgcggaagct tgctcgaaac
    25021 cttcagggga agagaggctg gtacccagaa gccacagcag gatgcccact gcaacaaagg
    25081 tgatcacacc ggaaacgcga tggagaatgg acgctatcgc cgtgatgggg aaccggatgg
    25141 tctgtaggtc cagattaaca ggtctttgtt ttttcacatt tcttatcatg aataacgccc
    25201 acatgctgtt cttattattc cctggggact acgggcacag aggttaactt tctgttacct
    25261 ggagacgtcg ggatttcctt cctccggtct gcttgcgggt cagacagcgt cctttccata
    25321 actgcgcgtc atgcaaaaca ctgcttccag atgcgaaaac gacacgttac aacgctgggt
    25381 ggctcgggat tgcagggtgt tccggagacc tggcggcagt ataggctgtt cacaaaatca
    25441 ttacaattaa cctacatata gtttgtcggg ttttatcctg aacagtgatc caggtcacga
    25501 taacaacatt tatttaattt ttaatcatct aatttgacaa tcattcaaca aagttgttac
    25561 aaacattacc aggaaaagca tataatgcgt aaaagttatg aagtcggtat ttcacctaag
    25621 attaacttat gtaacagtgt ggaagtattg accaattcat tcgggacagt tattagtggt
    25681 agacaagttt aataattcgg attgctaagt acttgattcg ccatttattc gtcatcaatg
    25741 gattctttac ctgcaagcgc ccagagctct gtacccaggt tttcccctct ttcacagagc
    25801 ggcgagccaa ataacaaacg ggtaaagcca ggttgatgtg cgaaggcaaa tttaagttcc
    25861 ggcagtctta cgcaataagg cgctaaggag accttaaatg gctgatacaa aagcaaaact
    25921 caccctcaac ggggacacag ctgttgaact ggatgtgctg aaaggcacgc tgggtcaaga
    25981 tgttattgat atccgtactc tcggttcaaa aggtgtgttc acctttgacc caggcttcac
    26041 ttcaaccgca tcctgcgaat ctaaaattac ttttattgat ggtgatgaag gtattttgct
    26101 gcaccgcggt ttcccgatcg atcagctggc gaccgattct aactacctag aagtttgtta
    26161 catcctgctg aatggtgaaa aaccgactca ggaacagtat gacgaattta aaactacggt
    26221 gacccgtcat accatgatcc acgagcagat tacccgtctg ttccacgctt tccgtcgcga
    26281 ctcacatcca atggcagtca tgtgtggtat taccggcgcg ctggcggcgt tctatcacga
    26341 ctcgctggat gttaacaatc ctcgtcatcg tgaaattgcc gcgttccgcc tgctgtcgaa
    26401 aatgccgacc atggccgcga tgtgttacaa gtattccatt ggtcagccat ttgtttatcc
    26461 gcgcaacgat ctctcctatg ccggtaactt cctgaatatg atgttctcca cgccgtgcga
    26521 accgtatgaa gttaatccga ttctggaacg tgctatggac cgtattctga tcctgcacgc
    26581 tgaccatgaa cagaacgcct ctacctccac cgtgcgtacc gctggctctt cgggtgcgaa
    26641 cccgtttgcc tgtatcgcag caggtattgc ttcactgtgg ggacctgcgc acggtggtgc
    26701 taacgaagcg gcgctgaaaa tgctggaaga aattagctcc gttaaacaca ttccggaatt
    26761 tgttcgtcgt gcgaaagata aaaatgattc tttccgcctg atgggcttcg gtcaccgcgt
    26821 gtacaaaaat tacgacccgc gcgccaccgt aatgcgtgaa acctgccatg aagttctgaa
    26881 agagctgggc accaaagatg acctgctgga agtggctatg gagctggaaa acatcgcgct
    26941 gaacgacccg tactttatcg agaagaaact gtacccgaac gtcgatttct actctggtat
    27001 catcctgaaa gcgatgggta ttccgtcttc catgttcacc gtcattttcg caatggcacg
    27061 taccgttggc tggatcgccc actggagcga aatgcacagt tacggtatga agattgcccg
    27121 tccgcgtcag ctgtatacag gatatgaaaa acgcgacttt aaaagcgata tcaagcgtta
    27181 atggttgatt gctaagttgt aaatatttta acccgccgtt caaatggcgg gttaattttt
    27241 atatgcctaa acacaaaaaa ttgtaaaaat aaaatccact atcggacatt catatattct
    27301 aaaaaaaaat atatcggtat aaattataaa caaatcgatg atttttaatc atctatcttc
    27361 ctctcagtta catgaataaa ccaccaaata tagcgcaccc ctataatttc caatacttta
    27421 ttcatcaaac atttaattca taccatcaat taattacttt tacatttaat aaaattaata
    27481 atatttttaa gaaaaactaa aaacattcga caatcaaata agatattctt gcaccgatat
    27541 acttttttct tttctgttaa aggactcagt tatgtttaaa ggacaaaaaa cattggccgc
    27601 actggccgta tctctgctgt tcacagcacc tgtttatgcg gctgatgaag gatccggtga
    27661 aattcacttt aaaggtgaag ttattgaagc accgtgtgaa atacatcagg atgatattga
    27721 taaagaggtt gaactcggtc aggtgaccac cagccacatt aatcagtcac atcacagcga
    27781 tgccgttgct gtcgacctgc tcttagtcaa ctgtgatctg gaaaactcca gcaacggttc
    27841 cggtggcaag atttctaaag ttgcagtcac ctttgatagc tcagcgaaaa ccaccggcgc
    27901 agatccgatt ctcaacaaca ccagcacggg tgaagccacc tgcgtcggcg tacgtttaat
    27961 gaataaagat caaagcaata tcgttttagg caccgctact ccagatatcg acctggctcc
    28021 gacctccagc gaacaaacgc tgaatttctt tgcctggatg gaacaaattg atcaggctac
    28081 acctgtaacg ccaggcgcag ttacggcgaa tgcaacctac gtgctggatt ataaataagt
    28141 attactttgg cggcgctatc gccgcctttc ataaaatatt tatcaggtat ggacaccgtg
    28201 aatatttatc gactctcttt tgtatcctgc ctggtcgtgg cgatgccttg cgcattggcg
    28261 ctcgaattca atctgaatgt tctcgataaa tcaatgcgcg accgcattga tatttcatta
    28321 ttaaaggaaa aaggagtcat tgctcccggt gagtattttg ttagcgttgc ggtaaataat
    28381 aaccaaatca gtaacgagca aaagattaac tggcacaaaa atgacgataa aaccattccg
    28441 tgcatcaatg atttactggt cgataaattt ggcttaaaac ctgaagtccg tcagtcatta
    28501 ccattgataa atcagtgcgt cgattttagc tcccgacctg aaatgctctt caatttcgat
    28561 caagccaatc agcaactgaa tattagtatt ccgcaagcct ggctggcgtg gtactcagaa
    28621 aactggaccc caccctccac atggaaagaa ggtgtcgccg gtgtcctgat ggattacaac
    28681 ttgtttgcca gcagctaccg cccacaggat ggcagcagca gcactaatct taacgcctat
    28741 ggaacagccg gaatcaacac gggggcatgg cgcttacgca gtgattatca gttgaatcat
    28801 actgatagcg atgataacca tgaacagtca ggcgaaatat cacgtaccta tctttttcgc
    28861 ccgttaccgc aattaggttc aaaattaacc ctcggcgaaa cggattttag ttccaatatt
    28921 ttcgacggtt tttcttatac cggcgcggca ctggcaagtg acgatcgaat gttgccatgg
    28981 gaactacgcg gctacgcccc acaaattagc ggtattgcac agaccaatgc cacggtgacg
    29041 atcagtcaat caggccgcgt catttaccag aaaaaagtcc caccaggccc atttatcatt
    29101 gacgacctta atcagtctgt tcagggcaca ctggatgtca aagtgacgga agaagatggt
    29161 cgggtgaaca atttccaggt ttcagcagca tcgacgccct tcctgactcg tcagggacag
    29221 gttcgctata aactggccgc gggtcagcca cggccctcca tgtcacatca aactgaaaat
    29281 gaaacctttt ttagcaatga agtttcctgg gggatgctgt caaacacctc gctgtacagc
    29341 ggcctgctgc tttctggtga tgactaccat tctgccgcaa tgggtattgg gcaaaatatg
    29401 ctgtggcttg gtgcgctgtc gtttgatgtc acgtgggcca gtagccattt tgatactcag
    29461 caggacgagc ggggcttaag ctaccgtttt aattacagca aacaagtgga tgccactaac
    29521 agcacgattt ccctcgccgc ttatcgtttc tccgatcgtc attttcacag ctacgccaac
    29581 tatctggatc acaaatacaa cgacagcgat gcgcaggacg aaaaacagac gatcagctta
    29641 tctgtgggcc aaccgattac cccactaaac ctcaatcttt acgccaacct gctacatcaa
    29701 acctggtgga atgcagacgc ctccacgacc gccaacatca cggcaggttt taatgttgat
    29761 attggtgact ggagagatat ctctatttct acgtcattca atacaaccca ttacgaagat
    29821 aaagatcgcg acaaccagat ttacctgtcg atttcgctcc ccttcggtaa cggtggtcag
    29881 gttggctatg acatgcaaaa cagtagccac agcaccacac accgcatgtc gtggaacgat
    29941 acgctggatg aacgtaatag ctggggcatg tctgccggac tgcaatccga ccgtcctgac
    30001 aatggagccc aggtgagcgg taactatcag cacctgagtt cagcgggtga gtgggatatt
    30061 tctggtacct atgccgccaa tgattacagt tccgtcagca gcagctggag cggttctttc
    30121 accgcaaccc aatatggtgc agcgtttcat cgccgcagct ccaccaatga accacgcctg
    30181 atggtcagca ccgatggcgt ggcagatatt ccggttcagg gcaatctcga ctacaccaac
    30241 cattttggca ttgcggtggt gccgttgatt tccagttacc agccttccac cgtggcggtg
    30301 aacatgaatg acttacccga cggcgtaaca gttgcagaaa acgttatcaa agaaacgtgg
    30361 attgaaggcg cgataggtta caaatcactg gcttcccgtt ccggtaaaga cgttaacgtc
    30421 atcattcgca acgccagcgg tcagtttcct cccctcggag cggatatccg ccaggatgac
    30481 agcggcatta gcgtggggat ggttggcgag gaaggacatg cctggttaag cggagtcgct
    30541 gaaaatcaaa agtttaccgt ggtctggggt gatagccagc attgctcgct ccatcttcct
    30601 gaacatatgg aagacaccgc aaatcgcctg attttacctt gtcattaatt aaggaaatga
    30661 aaatgacatt tatgaaagga ctaccccttt tgttgttggt tgccagtctg tgcagccacg
    30721 ctgcactaca acccgatcgc actcgtattg tgtttaacgc taatgataaa gctaccagtc
    30781 tgcgggtgga taatcgtagc gataaactcc cctacctcgc ttattcctgg ctcgaaaatg
    30841 aaaaagggga gaaaagcgat gatcttctgg tcgccctgcc gccgatacaa cgtctggaac
    30901 cgaaagcaac aacgcaggta cggatagtaa agcaagcctc gaccacaaaa ttaccgggcg
    30961 atcgcgaaac gctatttttc tacaatatgc gtgaaattcc tcccgccccg gaaaaaaaca
    31021 gcgaccatgc cgttctgcaa gtcgccattc agagtcgtat caaggtgttc tggcgaccgg
    31081 ctgcattacg caagaaagca ggagaaaagg tcgaactgca gttacaggtc agccagcagg
    31141 gcaaccaact gacactgaaa aatcctaccg cgtattatct gaccattgcc tacctgggac
    31201 gaaatgaaaa aggcgttctc cccggcttca aaaccgtgat ggttgcgcct tttagtacgg
    31261 tcaacacaaa caccggaaat tatagcggca gccagtttta tctcggttat atggatgatt
    31321 acggcgcgtt acgcatgacg acgctcaact gcagcggaca atgccattta caagccgtgg
    31381 aggcgaagaa atgagtgctg gcaagggagt gttgctcgtc atctgtctgt tatttctgcc
    31441 gttgaagtct gcgcttgccc tgaactgcta ctttggttca tccggtggct cggtagaaaa
    31501 atcagaagcg attcaacctt ttgctgtacc aggcaatgcc aaactcggcg ataagatctg
    31561 ggaatctgac gatattaaaa ttcccgtcta ttgtgacaac aataccaacg gcaactttga
    31621 aagcgaacat gtctacgcat gggtaaaccc ctacccgggg gtgcaggatc gttattatca
    31681 actcggagta acttacaatg gcgttgatta tgacgccaac cagggaaaaa gccgcatcga
    31741 cactaaccag tgtatcgaca gtaagaacat tgacatttat acccttgagc agatcatcgc
    31801 gatgggatgg cagaacaaaa tttgctcggg tgatcccgct aatattcaca tgtcgcgtac
    31861 ctttctcgct cgcatgcggt tatacgtcaa aatacgagaa atgccgccgc atgattatca
    31921 aagtacgctt agcgactata tcgtcgtgca atttgacggt gccggtagcg ttaacgaaga
    31981 ccctactgcc caaaacctga aatatcatat tactggtctg gaaaacattc gcgtgctgga
    32041 ttgcagcgtc aatttttcca tttcaccgga aacacaggtg attgattttg gtaaatttaa
    32101 tttgctggat attcgtcgcc acacaatgtc gaaaacattc agtattaaga cgacaaaatc
    32161 acaaaatgac caatgcaccg acggatttaa agtcagttcc tccttttata ccgaagaaac
    32221 gttggttgaa gaagataaag cattgttgat tggtaatggt ctgaagctac gtttgctgga
    32281 tgaaaacgcc tcgccttata ccttcaataa atatgctgaa tacgccgatt tcaccagcga
    32341 catgttggtc tacgaaaaaa cctatacagc tgaactttcg tccattgcag gtacccccat
    32401 cgaggctggg gcccttcgat acagtggtgc tttttaaaat taactataac tgatttgcca
    32461 tgatgccctt tgcagccgga aagggcatct ctttcagaaa attgctgatt ttcccctttt
    32521 ccttgattgc tgttattttt tcctagcccg gctttcttta tgctgtatcg atacaccaaa
    32581 gagaaaataa tgagggagcg aaggatgcca gttttgcagt ggggaatgtt atgtgtgcta
    32641 tcacttctcc tttctattgg tttcctcgcg gtacatctcc cggcggcgct attacttggg
    32701 ccgatgatcg ccgggatcat cttcagtatg cgcggaatta ctctgcaact tccccgctcc
    32761 gcttttctcg ccgcgcaggc cattcttggc tgcatgattg cgcaaaacct cactggttcg
    32821 attctcacca cgctggccgc caactggccg atcgtgcttg ctattttact ggtgacgctg
    32881 ctttccagcg ccatcgtggg ttggttattg gtgcgctata gctcactgcc tggaaatacc
    32941 ggtgcctggg gttcttctcc aggcggcgca gcggcaatgg tcgctatggc gcaagattac
    33001 ggcgcagata ttcgcctggt ggcgtttatg caatatctgc gagtgctgtt cgtcgcgggt
    33061 gccgctgttc tggtcacccg catgatgctg ggtgataacg ctgaagcggt taaccagcaa
    33121 atcgtctggt tcccgccagt gagcattaat ctcctgctta cgattttgct cgcagtcgtt
    33181 gccggtacgg cgggatgcat gctgcgtctt ccttccggca cgatgctcat cccgatgctg
    33241 gcgggcgcag tgctccagtc tggtcagctc atcaccatcg aactaccgga atggctgctg
    33301 gcgatggcgt atatggcaat tggctggcgg attggtcttg gtttcgataa acaaatctta
    33361 ctgcgggcac tacgcccgct accgcaaatc ctgctgtcga tttttgctct gctggctatt
    33421 tgtgcaggta tggcgtgggg actgacccgg tttatgcata ttgattttat gactgcctac
    33481 ctcgccacca gtcccggcgg gcttgatacc gtggcggtca tcgccgcagg gagcaatgcc
    33541 gatatggcgc ttattatggc gatgcaaacc ctgcgcctgt tcagtatttt gctgacaggg
    33601 ccagccattg cgcggtttat ttcaacctat gcgccgaagc ggtcggccta gtgctggcag
    33661 cctggacacc agtaaaacgg gcgcgaggac aacgtggttt tctcaatgat gccgccacag
    33721 cgttcgcaca gttcgccatc acgatgaaaa accttaaagc gaaacagcgc cccatgatgc
    33781 ttattctcat ccacctgccc ccgcgtagcg taagaaaacc gaggaatctc cagtaacgcg
    33841 tgtgcgagtg catccagttg cgccgcattg agatctttcg ctttatgatt tccagtcaac
    33901 ccaacctgcc agaggatctc cacccgcaaa taattgccaa gcccagccag aaacgcctga
    33961 tcgagcagta atcctgcaaa ctgacggtta cgaaagcgcg gcgacaataa ccgttccttt
    34021 accacctccg gcgtcagatt cggatccagc acatcgggac caacgcgttg taaaaacgga
    34081 tgcgtggtca gttgttccgg cctcaacatc tcaatatccg aggcgctata aagcagaatg
    34141 gttttgtcag ccgtttgcag ttttacccgc aatactcgcg tggtctgcgg ctcttcgccg
    34201 gtatcaacca cgcgccagac gccgtaaagc tgattatggc tgtagagcgt taaaccgttg
    34261 ggaaaatgag ttaacaacgc cttaccacgc gtttccacat gggtaacgtg ttgaccgata
    34321 agttgtgatt gataagtttt taactgcggg aaggcaaacc agacatcagt tagtggtttg
    34381 cctttgatcg ccgcctccag gttatccgct gcacggcgga tctccgggcc ttcaggcatg
    34441 gtgatatcct tttttcattc tttattaagc ggggttatgc tgcgacaaca atcccctttt
    34501 tggcaaatgt agctcgtaat cggcgggcga acgccagagc gtgctcgccg tcgccatgca
    34561 ggcagaccgt ttgcgccgcg accgttgccc attcgccggt gatacttttg actctgccgt
    34621 gttgcaccat ctccagcgtt tgcgccaacg cctgttcttc gttttcaatc aacgcgcctg
    34681 gctgacttcg cggcaccagc gagccgtcag cctgataacc gcgatcggca aacacttcct
    34741 cgcgcgttgt caggccatat tgcttgcctg cacgaatcag ctcgcttcct gccagcccaa
    34801 cgagaaccaa tgccggatcg caagcgtata ccgctctggc gatggcgtct gccagttgtg
    34861 cttctttcgc cgcctggttg tacaacatgc cgtgcggttt gacatgacgc attacgccgc
    34921 cttgcgcacg ggcaatcgtt gccagcgcgc caatttgata cagcgtctgg gcgtaaacgg
    34981 tttccggcgg cagctgcatg gcgctgcgac caaaattttc cctgtcggga aaactcgggt
    35041 gagcgccaat cgcgacacca ttttttattg cttcacgcac gcaagcctgc atggtttgcg
    35101 catcgcctgc atgaaatcca caagcaatat tggcagagga aaccagcgtt aatagctctg
    35161 cgtcgctggc gcagccttcg cccagatcgg cgttcaggtc aattttcatt gtgcagccgc
    35221 cacgctaatt gttcaaaata acgttgctga tcttgccgcg ctttcagtgc ctcttccagt
    35281 gaacactgga caaaatgaat cggctgaccg agcggaattt gcgccagatg gtacatatca
    35341 gcctcaatga tacaggcaat acgtggataa ccaccggtgg tctgtgcgtc gttcatcaac
    35401 acaatcggct gcccgttatg cggcacctgc accacgcccg gtaacaaacc gtgagataac
    35461 agttcgcgat cggtgatgcg ttttaaaatt tgcccctgta aacgatagcc cattcggtta
    35521 ctttgcgagc taagctgcca gggcgaacgc cagaatgcat cctgcgaggc gcgatcgaac
    35581 tcatgatatt ccggccccgg caaggcgcga atgcggttgc cccacagcag ctgtttaacg
    35641 ccctgcgctt ccataaaatc acgcttcgat ttgccaatcg ggagtcggtc accatccttc
    35701 agtaaacggc cttccagccc gccaatcccc actttgagat cggtgctgca tgaccccatt
    35761 accggcggaa catcaatacc acccgcgacc gccagataac tgcgcatccc gtgctgcggg
    35821 cgtttaagcg ttaaacgctg gcctgctttc atcggcaatc gccagccggt ccagacggcg
    35881 ttatcatcca accgcgcttc gcaacctgca cccgtcaggg caaaccaccc atcagtttcg
    35941 aactcaactg ttaactgacc gagcgtgatc tccagcgcgg gggcattggc gtcattaccc
    36001 accagcaggt tagcaatgcg cagcgcaggc atatccagtg cgccacagtg gctgataccc
    36061 gactggcgaa aaccgtgacg accgccatcc tgcacagtgg tgtacatgcc cgcacgaata
    36121 atcttcagca tattccctcc ttctgcggta caaatcgcac gctgtctccc ggacgtaata
    36181 agatgggttc gtcacgcgcc ggatcaaaca ggctgagtga ggtatgacca atcaactgcc
    36241 agccacccgg cgttgccagc ggataaacac cagtctgcgg cccgccgatc ccgacagaac
    36301 ctgccggaac gagcaagcgc ggttcagcgc gccgtggcgt gtgttgttgt tccggcaaac
    36361 tcccgagata cgggaagccc ggttgaaaac ctaaaaacca gaccacgtat tccacggagg
    36421 agtgcaattc aacaacctgt ttttcgctca acccgcaata cgccgcgacc accgccaaat
    36481 ccggtccgcc tgcaccaccg taaaccaccg gaatttcaat aaagcgagac tcaggttcca
    36541 gggcctcgct ctcctcccac cagcgttgca aacgctctat ggcatccagc gccagcgact
    36601 caggattacg caaaatcacc gtgatattgt tcatgccggg aatggcttca accacattcg
    36661 gcatatccac cagacgctgc gccagtcgcc agatccgctt ctggctagcc agcgtcaccg
    36721 gcggttccag ttccagcact accgccgttt cacctatcag ataacaacgc gctcgttgca
    36781 ctttcgatcc ctctgattat tcgttatgca ggattaggaa tatcaataaa ggtcacatca
    36841 agatcggtat tttcattcag ccactcgctc aatgcgcgaa taccaccgcg ttcagtggca
    36901 tggtgacctg cagcataaaa atgcaatccc tgctcgcggg ctgaatgaat ggtctgttca
    36961 gaaacttcgc cagtaataaa agcatccacg ccaaaacgcg cggcgctatc gataaaactt
    37021 tgcccgccgc ccgtgcacca ggcgacgcgc tggaccacct caggtccggt atcgccacac
    37081 cataacggct tacgtcccag acgcgcttca atccaggagg ctagttccat tcccggcaca
    37141 ggcatggtca gctctcccca cggcaccaac ggctcaattt cgcccatgac cgtgatcccc
    37201 agtaacgccg ccagctgtgc gttattgccc agctcaggat gtgcgtcaag cggcaaatgc
    37261 cagccataca ggttgatatc attcgccagc aacgttttta aacggttacg cttcattccg
    37321 cgaatgaccg gagactctcc tttccagaag tagccatgat gcacaatgac cgcatccgcg
    37381 cccagacgca ctgcctcatc gagcaaagcc tggctggcgg tgacaccggt aacaattttt
    37441 tgcaccgtct ctttgccttc cacctgcaaa ccattcggcg cgtaatcact aatcgccgcg
    37501 ctgttcagtt tttcgttaat cagttgttcc agttcggtgt ttttcatctt cgcgccttcc
    37561 gtattctgat agctacaccg ttaacatagc gttacctcgc ctgttcgtcg atagccaatc
    37621 ttttgacata ttaagaaaca aacccgacgc caaaagacgc aaattgttta tatcatcacg
    37681 cttcagctaa tgtatattta tgcaatcaga cagcatattg cataaataat cagttctgta
    37741 tcaaagtttt aactttaagg aaacacaagc acgccgcgca actattacgt gacgttgccg
    37801 tcaattgtgg aattattatg aataaacacg catcacaacc gcgcgctatt tactatgtcg
    37861 ttgcgctgca aatctgggaa tattttagct tttacggcat gcgtgccctg ctgattctct
    37921 atctcaccaa tcaactaaaa tacaacgata ctcacgccta cgagttattt agcgcctact
    37981 gttcgctggt gtatgtcacg ccaatcctcg gtggcttttt ggcggataaa gttctcggca
    38041 atcgcatggc ggtgatgctg ggggcgttgt tgatggcgat cggtcatgtg gtgctgggtg
    38101 ccagtgagat ccatccgtca ttcctctatc tgtccctggc gattatcgtc tgcggctatg
    38161 gcctgtttaa atctaacgta agctgtctgc tcggcgagct gtatgagcca accgatccgc
    38221 gtcgtgatgg cggtttctcg ctgatgtatg cggcgggtaa cgtggggtct attatcgcac
    38281 ctatcgcctg tggttacgcc caggaagagt acagctgggc gatgggcttt ggcctggcgg
    38341 cggttggcat gatcgcgggt ctggtcattt tcttatgtgg caatcgtcat ttcactcata
    38401 cccgcggcgt taacaaaaaa gtactgcgtg cgacaaactt tctcctgccg aactggggat
    38461 ggctgctggt tctgctggtg gcaacgcctg cactgattac cgtgctgttc tggaaagagt
    38521 ggtcggtata cgccttaatt gtcgcgacta ttattggcct gggtgtactg gcaaaaattt
    38581 atcgcaaagc agaaaaccag aaacagcgga aggagctggg gctgattgtg acgctcacct
    38641 tattcagtat gttgttctgg gccttcgcac aacagggcgg tagctcgatt agcctttata
    38701 tcgaccgctt cgttaaccgc gatatgtttg gttataccgt tccgaccgcg atgttccagt
    38761 cgattaatgc cttcgcagtt atgctgtgcg gtgtgttcct ggcgtgggtg gttaaagaaa
    38821 gcgtcgcagg taatcgtacc gtgcgcatct ggggaaaatt tgctcttggc cttggcctga
    38881 tgagcgccgg attctgcatt ctgaccttaa gcgcccgctg gtccgcaatg tatggtcact
    38941 cttctctgcc actgatggtg ttaggcctcg cggtgatggg ctttgcggaa ctgtttatcg
    39001 acccggttgc catgtcgcaa attacgcgca ttgaaattcc gggcgtgacc ggcgtattaa
    39061 ccggcatcta catgctgctt tctggcgcga tcgcgaacta tctggcgggc gtgattgccg
    39121 atcagacatc gcaggcttcg tttgatgctt ccggggcgat caactactcc atcaatgcat
    39181 atattgaagt atttgatcaa attacctggg gcgcactggc gtgtgtagga ctggtactga
    39241 tgatttggct gtatcaggcg ctgaaattca gaaaccgcgc gctggcgctg gagtcataat
    39301 aggccagcac cgatgcctga tgcgacgctt gacgcgtctt atcaggccta cgtatttcct
    39361 gcaatttatt gaatttgcac aaatttgtag gctggattag gcgttcacgc cgcatccggc
    39421 atctgggctc gattgcctga cgcgtctgtt atttcccctt ccgcgccgtc tcatacgctg
    39481 ccaacgtttg tactctcgcc tctttgtgct cgactatcgg ttgcggataa tccagcttca
    39541 cacctgcttt ctgcgcccac ttccacggct catgcaccgc tttccctggt acatcgcgca
    39601 gctccggtaa ccatcgacga ataaactcgc cctcacggtc aaatttctcg ccctgagtta
    39661 taggattgaa aatacgaaaa tacggcgcag catcagtacc cgtagaagcc gcccactgcc
    39721 agccaccgtt attagctgcc aaatcaccat caatcagctg cgacataaaa tatcgctcgc
    39781 cttcgcgcca gtcgatcaac aaatctttca ccagaaaact ggctgtaatc atccgtagcc
    39841 ggttatgcat ccagccagtg ctgttaagct gacgcatagc ggcatcaata atcgggtatc
    39901 ccgttttgcc tttctgccag gcctgtaaat gtgcgggatt gctttgccac tgcacacgat
    39961 ccgtccaggc aataaatgga cgatgtttac acaacgaggg gtaatacgtc atcagatgac
    40021 ggtagaactc gcgccagatc agctcactaa gccagacact accggcccca ccgtccagcg
    40081 cctgcggctg ttccgccaac aagcgatgca aacactggcg aggcgataac ccgcccgttg
    40141 ccaagctggc ggacaaacgg ctggtgcctt ccactgccgg aaaatctcgt tgttgctcat
    40201 actctccagc accgttctcg caaaactggc gtaattgcgc aatcgccgct ttttcttcca
    40261 ccgggaaatg cgcagtatcg aaagactgac gaggataatt cagcgtaatg gatggcgcgg
    40321 gctttatcga tccgctacta cgaacttttg gtgcagcgac gcactccggc atcccttccc
    40381 gcagccgttt cagccaggca ttcttaaaag gcgtaaagac tttgtacatc tcgtgattgc
    40441 cggtcatcac cgcccccggc ggcaggatca cgctgtcatc aaatccttca cacaccacgt
    40501 tacgcagcgc tctttcaacg tccacatccc gcgctcgctc attcacctca tactgatagt
    40561 tataaaaaag atgagtaacg ctgttttccg cgcacacctg tttaactatt tcgacactgg
    40621 cgacaaagtc atccacttca cggaacaata aaggaatacc tttttccgcc agcgcgattt
    40681 gtagcccatt cagttgagca ttgataagtt cagcctgacg cggcgacatg ttatgcgtct
    40741 cccactggcg tggtgtagcg atatacagcg ccagcacgcg tgcagacgaa ttgcggcagg
    40801 cggcagccag tgcgagatta tcgtgcagac gtaaatcctg gcgaaaccag gccagatggg
    40861 tagtcataaa actcctgaat ggcgcaagtt aatcacacgc ggccaaagtt cgaaataacg
    40921 ctgccctgaa agccaggcgt caggataaag cgccatatag tgtttgatac gcatcagcgg
    40981 cgcaagaagt ggttgctcgc cacggcgata actgtcgata agcgcagcca gctgctggcg
    41041 ttctgtggaa tcaatatgcg ggcgaaaata accctgaacg tgcatcaaaa cgttggtgtg
    41101 atcgcgcggg ttggcgggat gagaaagcaa aacaattacg cgttggcggt actggttgta
    41161 aaaatcatcc agattttgcc actgatgaat atcagccaca aacgggccaa gtttgcggta
    41221 ctcgggctga gaatgggcca gaaagacgag tttatagcgg ctgtgataat cgagaagcgc
    41281 cccgcgcgtt aagccatgtt ctttcagtcg atgtaactca tgaagggcga agatacggat
    41341 tagggtgctg tcatcaaatc gttgtagatt cattactgct cctgaacggt atacaggagt
    41401 aagtgtagac gttggtaaat gatgtttgtg aagttaatcc gctagcaggg caagattacg
    41461 aaagcgcgct ccccgcaagc aactaatcaa ggtagttaat catggctgaa cacgatatta
    41521 cgccagaagt caccatttcc aaaacacagc gccgctataa agtcggctat gtcagagccc
    41581 gccaccagga ccgcaaaacc ggtatagccc ggtattatag cctgcacctt aagggcgact
    41641 ggctgaaaga ggccggattt gataccggac ggggtgtcac ggtgaagatt tcggaggggg
    41701 gcctgaccat catcgctgac agtgacgagg tgcaggaact gcgggaggaa ctttatcagg
    41761 ttaaacaggt agttaagggg atgcgggatg ggatgtttag tgtgctgaac gaaagttaaa
    41821 tattaaaaaa gctgggaaga tttaaaacct tcctagcttt ttattctgtc ttattataat
    41881 gtatactcat catcttccga tgaatttata aattgctcca agtttacatg aagagtactt
    41941 aacctagttt ttaactcatt aagtgaagcg ataatttcat attttccaac ataatcatat
    42001 gatggatcgt attcattatc tatgtcagaa gctaaaaatg atgatattcg tagtttaaat
    42061 ttagcttcat ctttattaca ttcattggca taaggtgaat gccctatcat agcatattcc
    42121 tctaatctaa aaggataaga tggttttaat tcatcgatca atgaaatttc aatccatgtt
    42181 ctatatccag gcattaatgg actaatttct ttgagcaaaa cctttccact taataatgta
    42241 ttgagattgt tcattattat gtctccctcg ggaaaaatat atgtctaaca cttccaccat
    42301 gttgatgaac ttgtaaatgt ggtaaatcac catcaggatt tcctggtcca tgacctgaca
    42361 ctctatatgg aggttttgtt acagaaggat cttttatatc atcccaatga tatgaacctg
    42421 cttttgtttt atttccagta agatattcca ttaagttctt agtgctgggg cctgactcac
    42481 ctgtcatatt tttataatta tgacctaaat atctatccat aaacaattgt tcagcgtcac
    42541 ttctagacct aacagtaact ttggtttcac tactatctaa taaacgattc gctttttggt
    42601 tactaacagc acatggagtt aatccaagtg gatcagcata ttttagagga ttaggtgcat
    42661 aactgtagag atttaaaccg cctctcaacc ctatcgggtc agttaccatg aaccgcccga
    42721 catccggatc atagtacctg tgcagattgt agtgcagccc cgtttccctg tcgaggtact
    42781 gtccctggta gcgcaggttc tgctccaccc cgccgtggtc cttctcctgt atcgtgttac
    42841 cccagacctg ataccccgtc tcccacacga ttttcccgtc gctgtccgtc atctcttccg
    42901 gcgcgccgtt cacatccgtg tgaaaataca gtatccggtt cgggccctct ttgcccgcct
    42961 tatccacccg cgccagcggt tcataactcc cctggtccct gtagatgtac aggctgcctg
    43021 ttccctccgg cctgctctcc tgcgccagcc gcattccgtc ccataaaaat tttgtccggt
    43081 tgtacggctt accgtcaggg ctgatgtgct gtttttccgt ccgcctcccc agtgcatcgt
    43141 acacataccg gtatgtcccc cgcgctgtcg tcacttccac catccggtgt tccgcatcat
    43201 aacggtaact ctgcgttccg ctccggccct ccttttccac cgcacggcca aattcgtcat
    43261 aacggtagtg cacaccacgg taagacatca gccggttgaa cggcaccacg ctgttttctg
    43321 ccgtggccct ttcccccgcg cgcctcccca gcaggtttcc cgccgcgtcg tactgccagc
    43381 tcgccagata cccctcatcc tccgaacgac acgccgtgat gctccccgtg gggtcgtaga
    43441 agtaatccgt ttccccccgc cggctgtgcc gcgtcttcag cagttcatcc tcgccactgt
    43501 agctgtactg ccggtccgtg aacgtctccg gtaccacccc gcgcatcccg ctgtatgtct
    43561 ccctgcgctt cagccgcccg ccggggtcgt aaagctgccg cgtgctcagt cgcccctggc
    43621 ttctgagcac ttcacggtgc aggtggtcgc gcgtgaactc gctgatgacc tcccggtcca
    43681 gatttatctg ctggagatgg ccgctgccgt agtacagatg gcgctgcgtt ctgccgtccg
    43741 gaaaggtggt ggcagtccgg ttgcccagtt catcgtactg gtgggtcagg atgccctgcg
    43801 ccgtctcctc gctcagcatc cgccccagtc tgtcgtagct gaagcggata acttccggcg
    43861 cgtcagtttc gtgccgctgg cgacggattt ccagtagctg gtctgccgca tcatggacgt
    43921 attccgtacg gccatcggct gtctcttttg ccgtgagccg gcccgccgca tcacgcgcca
    43981 gccggtggcg gatttcaccg ccccggtccc cggtgaacac cacttccgtg accgcgttca
    44041 gcgcgttgta gcggtacgac tggcagcagc cgtccgggtt tatctgctcc gccagacggt
    44101 ccagcacatc atggcggaac cagtagcgtt ctccgttctc gttcgtgagc gtgaccagac
    44161 gcccgtaagc gtcgtacccg aatgccgtcc tgcgccccat cgcgtccgtc cggctgcgca
    44221 cccggccctg cccgtcccgc tgccagcgcg ttatcctccc catcgggctg gtgtacttta
    44281 ccagcctgcc cgccgcgtct gactggtaat tcaccgtgcg gccgtccgcc agtatcacct
    44341 ccgtcagatg accgctgtcg tcatacccgt agcgcgtgct gtggctctgc gcatccgtca
    44401 ttctgataag cctcgtgcgt tcgtcgtaga accacgccgt ctgcgagccg gaacagtccg
    44461 tgtgccgcat gagctgcccg tcttcattcc actggaggta tttattcccc ccgcgcgcat
    44521 ctgttatctg caccacctgg ccgtggtggt catactgata ccgggtgttc tgctgcagcg
    44581 ggtcggtcac ggcaagaagg ttgccgcgtt catcgtacac acagcgccag gtgtttcccg
    44641 ccgcgtccac ctccgtttcc ggctggtgcc acaccgggtg ccactgcgcc tgctccaccc
    44701 gccccagcgg gtcgtgcgtc tccgtgatat gcccctgcag gtcatacaca tagcgccatt
    44761 tgccgccctg agggtcggtc atccccagca acagtcgttc ttcgtcgctc cagcggaatg
    44821 tcgtcacctg tccggcttcg tcctggtacg ccgtcacctg attctgcgca tcccactggt
    44881 gataacgtgt gcgccccagt ccgtccgtca cgcacagcct gcgctgcgcc aggtcccacg
    44941 ccagccggta ctgttcaccg ctgtccgtgt ggtgccccgt cacccgccat gccgcgtcat
    45001 ccggaccttc ataccagccc cactggtaat aatacccttc gccgcccggc tggcggtgca
    45061 gcgtcatgca gtggttttcg tcgtaagcaa actcacgcag caggtgcccc gtgctgtcgt
    45121 acacgccgtt caggtggccg gcctcgtcat agctgtaacg acgcagcagt aaaggtgcag
    45181 tctcagggaa atgctgataa atctgtgtca cccgccgggg atgcgccgcc agttcgtaat
    45241 acagacggat gcacggacgc tgctgttcgc cgctgatttc cacaatgcgg ccccggtcgt
    45301 cgtagtacag attcagacag ttgctgttac ggtcgcccag cattttcagc cgctggcgct
    45361 gcgggtggtg cgggtcagcc tcaaacagaa aacactgccc gtcatcgctg ctgataagcc
    45421 agtgcccctg ctcaccacgc cttaccgcca gcccttcacc ggggctgtaa aacccgcccc
    45481 ccggcagtac ggcttccata tccaggcgtc gccccatgcc cgacacaaag gtcatgcagt
    45541 tttcgtctgc gctacccggt gtgcacacga ggctgacttc atacattaca ctccagccct
    45601 gaccaaacat tccttccgta cgttcgtcac gactgctgta cacgcgctgc cagtccagcg
    45661 gaaagtggcc cggcagactg aaatcgaagt catcgtcatc ggccaggtat ttggccccgg
    45721 tggcggcatt gaccggatgc ggaagggcac tggtggcctt ctgcgtcagc atgctcatcc
    45781 cgacactggc agcaaaacag gcgattttag aaagcatttt gcccgggcga agaaacatca
    45841 gcgccacggt tgccaccagg gttatctgcg acctgccgct tctgatatcc ctgaccacca
    45901 gtaacggacc gccaatgcgc acatccggtg acacaaacgc cccgttcccc ggctcattca
    45961 ccaccctggc ctcgcaggta cagcgtgccc cgctgcgtgt cgccggctgg ctgttgatac
    46021 gcacatcccg cgcgccttcc gcgatatatt ccgctgcggg cggctcgtca tcgcctttga
    46081 acagattact gatgctgcca ataaccgcat tcactccctg aaaggcggtt tccagcatac
    46141 cgcccggatt cagcaggtta agggccgttc ccagggggtc atccagaaag gcggcttttt
    46201 tttgtgcgag aaactgcgtc agtgaggaag gatgtttatg gcagtcgatt ttatcgtcag
    46261 gggtcggggt ggagcccggg gccgcactgg ccacagtggg ctgccatagt tccgacacaa
    46321 aatgtgtcac gccatccacc atttcagaac gcccttcttc cgtactgagc tggtcgtgca
    46381 gagcccggga catcagcaca ggccctgtca ccgggttaac cgccagggct atggcccccg
    46441 ccatttccag atattccccg gccacatccc ggaacgttct ggcatcctcc tgacgctgcg
    46501 cttcttcctg ttctgccttc gcctgcgctt cctgcgcctc tatgtcctgc cgcgatgccg
    46561 cagtcgccgc tgcccgcgcg gcaggtttgc tgttgatcca tgtgtcacca gagccggtga
    46621 ggatgtaacc ttcaatctga ggcgggaaaa gcatatttgc gagggctgaa caggcttcgc
    46681 tgaccttttc tgtcacgccg gtgtacacca tcgccacacc cgccacaaaa ccggtcagca
    46741 cgacggccgt gccccccatc gtgatcccca ctgacgtcgc cagtaacgag gttgtgccga
    46801 caatgagggc atcaataacg acatttgcag ccacctccag caccgccccc agcacatctg
    46861 ccataaacga cgtatgcagc agggcatccc cctgtcttgc cgcccagaat gcctcttcca
    46921 tgtcccgttc cctgtatgat taattcgtcc tgacttcctg ccgcatcctg aagcgcagat
    46981 tctttttaat ttcagcccag cgcgtcatat ctgccggggt cagcggtgtg gttctggcgt
    47041 aggtgaaggc catcatgacc cgctcacccg gcaatacaaa tgccacctga cactggtaca
    47101 cctgctcgtt accccggaga aactggctct gtatttccaa accgcctcgt tgagataata
    47161 ttccgtcata atgtaaatag tccgttttaa taaaggaatt atttattgac aggttctgtt
    47221 tttttaatca tgaatgtaat acccatcacc gctttacagc acactttatc tttccagcca
    47281 taatcactaa taatattaag caattagcaa tatttacaac gaccagagcg gatacatagt
    47341 tatgtattta acaatcccaa tatattcact atgcaaataa aaccactagt aacagacacg
    47401 ctaccgcctc catataaaaa tggacacctt ataaaattgt atcttgcata gaaaaagctg
    47461 aaatatcgga tgcgttttaa ttatcaacta taaatctata agctttttat taacaaca
//
LOCUS       DADMGC010000015        44108 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.015, whole genome shotgun sequence.
ACCESSION   DADMGC010000015 DADMGC010000000
VERSION     DADMGC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44108
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.015"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(1305..2987)
                     /gene="glcA"
                     /locus_tag="JNZ73_000861"
     CDS             complement(1305..2987)
                     /gene="glcA"
                     /locus_tag="JNZ73_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HAY8731245.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFEPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFAGVIGVITLLQAYVFTGMLVS"
     gene            complement(3342..5513)
                     /gene="glcB"
                     /locus_tag="JNZ73_000862"
     CDS             complement(3342..5513)
                     /gene="glcB"
                     /locus_tag="JNZ73_000862"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HAY8731246.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGIVPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5535..5939)
                     /locus_tag="JNZ73_000863"
     CDS             complement(5535..5939)
                     /locus_tag="JNZ73_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HAY8731247.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(5944..7167)
                     /gene="glcF"
                     /locus_tag="JNZ73_000864"
     CDS             complement(5944..7167)
                     /gene="glcF"
                     /locus_tag="JNZ73_000864"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HAY8731248.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVENVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            complement(7178..8230)
                     /gene="glcE"
                     /locus_tag="JNZ73_000865"
     CDS             complement(7178..8230)
                     /gene="glcE"
                     /locus_tag="JNZ73_000865"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HAY8731249.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFCYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8230..9729)
                     /gene="glcD"
                     /locus_tag="JNZ73_000866"
     CDS             complement(8230..9729)
                     /gene="glcD"
                     /locus_tag="JNZ73_000866"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HAY8731250.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKESLDINPVGRRARVQPGVCNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNYILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANELGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            9975..10745
                     /gene="glcC"
                     /locus_tag="JNZ73_000867"
     CDS             9975..10745
                     /gene="glcC"
                     /locus_tag="JNZ73_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HAY8731251.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10965..12011)
                     /locus_tag="JNZ73_000868"
     CDS             complement(10965..12011)
                     /locus_tag="JNZ73_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8731252.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQDFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEM"
     gene            12415..14103
                     /locus_tag="JNZ73_000869"
                     /pseudo
     CDS             12415..14103
                     /locus_tag="JNZ73_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            14118..15014
                     /locus_tag="JNZ73_000870"
     CDS             14118..15014
                     /locus_tag="JNZ73_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAY8731253.1"
                     /translation="MTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNLTWVRGS
                     LEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGFCQRFLF
                     ISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKPLFDWML
                     RGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDWQRVRQL
                     AAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADWSASNNR
                     ISEDINWFPGISLEHALRNGLF"
     gene            15047..15295
                     /locus_tag="JNZ73_000871"
     CDS             15047..15295
                     /locus_tag="JNZ73_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY8731254.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLNVKPPAQLMETLLPWLENK"
     gene            15295..16467
                     /locus_tag="JNZ73_000872"
     CDS             15295..16467
                     /locus_tag="JNZ73_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY8731255.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFVVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISPGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(16503..17582)
                     /gene="lptG"
                     /locus_tag="JNZ73_000873"
     CDS             complement(16503..17582)
                     /gene="lptG"
                     /locus_tag="JNZ73_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY8731256.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17579..18649)
                     /gene="lptF"
                     /locus_tag="JNZ73_000874"
     CDS             complement(17579..18649)
                     /gene="lptF"
                     /locus_tag="JNZ73_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY8731257.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRTWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPWQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18680..19240)
                     /locus_tag="JNZ73_000875"
     CDS             complement(18680..19240)
                     /locus_tag="JNZ73_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY8731258.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLSNE"
     gene            complement(19252..20076)
                     /locus_tag="JNZ73_000876"
     CDS             complement(19252..20076)
                     /locus_tag="JNZ73_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731259.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(20076..21425)
                     /locus_tag="JNZ73_000877"
     CDS             complement(20076..21425)
                     /locus_tag="JNZ73_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAY8731260.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21471..22229)
                     /locus_tag="JNZ73_000878"
     CDS             complement(21471..22229)
                     /locus_tag="JNZ73_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731261.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22261..22974)
                     /locus_tag="JNZ73_000879"
     CDS             complement(22261..22974)
                     /locus_tag="JNZ73_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY8731262.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23148..23840
                     /locus_tag="JNZ73_000880"
     CDS             23148..23840
                     /locus_tag="JNZ73_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY8731263.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23889..25388)
                     /gene="pitB"
                     /locus_tag="JNZ73_000881"
     CDS             complement(23889..25388)
                     /gene="pitB"
                     /locus_tag="JNZ73_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HAY8731264.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25680..27539)
                     /gene="gss"
                     /locus_tag="JNZ73_000882"
     CDS             complement(25680..27539)
                     /gene="gss"
                     /locus_tag="JNZ73_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HAY8731265.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVGHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27744..28610
                     /gene="yghU"
                     /locus_tag="JNZ73_000883"
     CDS             27744..28610
                     /gene="yghU"
                     /locus_tag="JNZ73_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HAY8731266.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28733..28981)
                     /gene="hybG"
                     /locus_tag="JNZ73_000884"
     CDS             complement(28733..28981)
                     /gene="hybG"
                     /locus_tag="JNZ73_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HAY8731267.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29115..29456)
                     /gene="hypA"
                     /locus_tag="JNZ73_000885"
     CDS             complement(29115..29456)
                     /gene="hypA"
                     /locus_tag="JNZ73_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY8731268.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29449..29937)
                     /gene="hybE"
                     /locus_tag="JNZ73_000886"
     CDS             complement(29449..29937)
                     /gene="hybE"
                     /locus_tag="JNZ73_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HAY8731269.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29930..30424)
                     /locus_tag="JNZ73_000887"
     CDS             complement(29930..30424)
                     /locus_tag="JNZ73_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HAY8731270.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLSDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALELVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30424..32127)
                     /gene="hybC"
                     /locus_tag="JNZ73_000888"
     CDS             complement(30424..32127)
                     /gene="hybC"
                     /locus_tag="JNZ73_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HAY8731271.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVSGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32124..33302)
                     /gene="hybB"
                     /locus_tag="JNZ73_000889"
     CDS             complement(32124..33302)
                     /gene="hybB"
                     /locus_tag="JNZ73_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HAY8731272.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33292..34278)
                     /gene="hybA"
                     /locus_tag="JNZ73_000890"
     CDS             complement(33292..34278)
                     /gene="hybA"
                     /locus_tag="JNZ73_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HAY8731273.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34281..35399)
                     /gene="hybO"
                     /locus_tag="JNZ73_000891"
     CDS             complement(34281..35399)
                     /gene="hybO"
                     /locus_tag="JNZ73_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HAY8731274.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35588..35875)
                     /locus_tag="JNZ73_000892"
     CDS             complement(35588..35875)
                     /locus_tag="JNZ73_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HAY8731275.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35994..36920)
                     /locus_tag="JNZ73_000893"
     CDS             complement(35994..36920)
                     /locus_tag="JNZ73_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708817.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY8731276.1"
                     /translation="MLCLKKHQLRNATMPRLTAKDFPQELLDYYDYYVHGKISKREFL
                     NLAAKYAVGGMTALALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLV
                     KPAKMSGKTPAVGVVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKG
                     RELQQQVDPTKLMNDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACA
                     VPFYGRQAPTADVAKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVN
                     HGFHNDSTPRYDKSAADLAWQRTLKWFDKYLS"
     gene            37038..38078
                     /gene="gpr"
                     /locus_tag="JNZ73_000894"
     CDS             37038..38078
                     /gene="gpr"
                     /locus_tag="JNZ73_000894"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAY8731277.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKIRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38118..38612)
                     /locus_tag="JNZ73_000895"
     CDS             complement(38118..38612)
                     /locus_tag="JNZ73_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HAY8731278.1"
                     /translation="MERFFENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38803..39688
                     /gene="yghA"
                     /locus_tag="JNZ73_000896"
                     /pseudo
     CDS             38803..39688
                     /gene="yghA"
                     /locus_tag="JNZ73_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
     gene            complement(39960..40385)
                     /gene="exbD"
                     /locus_tag="JNZ73_000897"
     CDS             complement(39960..40385)
                     /gene="exbD"
                     /locus_tag="JNZ73_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HAY8731279.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40392..41126)
                     /locus_tag="JNZ73_000898"
     CDS             complement(40392..41126)
                     /locus_tag="JNZ73_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbB"
                     /protein_id="HAY8731280.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41378..42565
                     /gene="metC"
                     /locus_tag="JNZ73_000899"
     CDS             41378..42565
                     /gene="metC"
                     /locus_tag="JNZ73_000899"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAY8731281.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42705..43364
                     /locus_tag="JNZ73_000900"
     CDS             42705..43364
                     /locus_tag="JNZ73_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8731282.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43404..>44108)
                     /locus_tag="JNZ73_000901"
     CDS             complement(43404..>44108)
                     /locus_tag="JNZ73_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8731283.1"
                     /translation="RVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLNVDILQLQ
                     ELLMDIGEDEHFQPSMAASGINSATLSEEILFAAERLLDVMERPLDARILGKQIIREI
                     LYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEANMSVSAFH
                     HNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSREFKRYFG
                     VTPGEDAARMRAMQGN"
BASE COUNT        10942 a        11762 c        10836 g        10568 t
ORIGIN      
        1 cgtaccgttt aaacaaagac aatacgtctc tttatttctc ataaaacccc aatcccttac
       61 agggcttatt gaaaaattta aaaatcacaa atttctcaat actcgaaatg agatcacata
      121 agaaatcaaa aagccttctc atctatttta agttttccca acggaaaata agagctttca
      181 ttaatactga accactttac tgttctattt gttcaggtat tgcattacgg tgtgctgaga
      241 tccatcggtg catccctgta tcccgcgatg agactaaaat agtcgtgatg taagctcgta
      301 tcagattctt agtgaatacc acatttgtta taattaagtg aaaagataca ttgctgattt
      361 acattatttg gtttacaaaa tgtcatcact tgcagagtta tcgctgacag atacctttaa
      421 tctcttacta ctttcgtgtt attgcaatac aaccatccgt aaagcagggc tatttttagc
      481 aggaaatact caggtgtcaa cattcgaact ccaactctga aatattcata taaattaatg
      541 attttaatga ttttttcatt tcttcgcgcc taataaaagt attcaaattc agatgatttt
      601 tctgctcaac agacacaaac aggcaataat actctccaaa gcgaacaata tttggcatta
      661 cgatttaatg cttcataatc accaaattat ttaaccataa attttcttat ttctaacaaa
      721 atgattggtg tgttgttgga gttaaaatac atacataata ttaacatctg ccaacatttc
      781 actttccgca agacggatga tttcgaatcc tcatccacgt taccctatcc caaatatctt
      841 tattaatagg cagtaacgaa cactgttata gcaagattaa cttattaacc taacggcatc
      901 ttttatgtaa caagtggaga taaattgctt aaaattgatc ctcgacaaat cttaaaagtt
      961 ctcattattc tgcaaaatca gctttgcgaa taatcttatg tttttgggag ttttgggaat
     1021 tttgatctac tcattgttct agtgaaaaat ataaataaga gaaaaaacgc cattcgtcat
     1081 gaaagcaaca gatgaagcaa tgtaataaca tactaaaatt gatctcatga aagtgggtac
     1141 tttataacca gcacagaagc caagttgtat aaatattaaa caattcgcat cgtgaaacgg
     1201 agactacgta taaagaagcc aatcgtaaaa acggttccct ggcgagataa acggcagaca
     1261 aatggaaagc cgggtgatat ttaccttcat ccggctttat tgatttacga gactaacatc
     1321 ccggtaaaca catacgcctg cagcagggtg ataacgccga taacgccggc aaaaatcaga
     1381 ctgtgcttca cggtgtagcg gaacagttca gattctcggc ccaccatgcc cgtcgcggcg
     1441 caggccacgg cgatagattg cggggagatc atcttgccag ttacgccgcc gctggtattt
     1501 gctgccacca gcagagtgtc agagacgttg atttgctgcg ccgtggtcga ttgcagtgaa
     1561 ccaaacaggg cgtttgaaga agtgtccgac ccggtaagga atacgcccag ccagccgaga
     1621 aacggtgaga aaaacgggaa catcacgcct gtacctgcca gtaccagcgc cagcgtggtg
     1681 gacatgccgg aatagttggt gacgaaggcg aatgccagca ccatgccaat cgacaatatc
     1741 ggccacttca gactaattag cgtttcggca aagacgccaa tccctttctt gatgcccacg
     1801 ccgaggatga agatagagat aatcgccgca ataaaaatag cggtgccgcc agccgagaga
     1861 ggttcgaatt taaacaccgc atccattggc gttggttggg cgacaatggg tgccgctttc
     1921 aacacttgtt gatgcaaatg agggatctgg aaattaatca ccagtgaata aaacgcgccg
     1981 cccggagcaa ataacgcttt aaacggcttc atggtccaga tggtgaccag caccgttaag
     2041 attaaaaacg gtgaccacgc acgaatgatt tgccccagac tatattctga aggcacggga
     2101 ccgccagaag atggcttatt taccaccatc gcacctgcgg attgccccat gctgattgcc
     2161 gtttcggtat ttttcggccg ccagacttta aggaataaag cgagtgagac gatactcacc
     2221 agcgccgaag taatatccgg cagttccgga ccaatatagt tagaggtaaa gaactgagtg
     2281 acagcgaagc ttcccccagc aaccagcgcc gctggccacg tctctttcac ccctttccag
     2341 ccgtccatca ttgctaccag ccagaacggc acaagaaccg acaggaacgg taactgacgt
     2401 cccgccattg cgccaatgtg gaacggatcg attcccgtta cctgaccggc gaccagaatc
     2461 ggcacgccca acgcaccaaa cgccaccggc gcagtattgg caatcagaca cagccccgcc
     2521 gcgtataacg gtttgaagcc caggcccacc agcagcgcac cggtaatcgc caccggcgca
     2581 ccaaagccag ccgctccttc cagcaacgca ccaaaggaga aaccaatcag taacacctgc
     2641 aaacgctgat cgtcggtgat ggagataacc gagctgcgga taatatcgaa ctgcccgctg
     2701 gcaacggtta atttatacag gaacaccgcc gcgacaataa tccacgcaat tggccataat
     2761 ccataaataa agccatagcc cgcagcagca aatgccatat caatcggcat tttaaaggcg
     2821 aatatcgcaa tcaggataga taatataagg gttattgctc cagcgacatg tcctttcaga
     2881 cgtaataccg cgagtgcaac gaagaagaat attatcggga tcagggcgac cagagcggat
     2941 agccccagtc ctcccatcgg catatacatt tgggtccagg taaccatatt gttcagtctc
     3001 ttattatctt tatatgcttg atatacttaa ggttgtaata agcaaaagag gactgaactg
     3061 taaaatatag gcgttatact ttacagcaac agtacgccgc taacgcaatt gctacctctg
     3121 gcataacaag tatatcgggt aagggtttct gttccgcaca cgcaggcgca gagtatcgtt
     3181 aagatgtcca tattgttgtt ttaggcccgc tagtaatgcg ctacgggtat ttaatattgt
     3241 taaaccctga taatcgctcc ggttatttcc gggataaatg tactaccgca gttactatca
     3301 tagccccgac aataaaactt gccggggctt ttttgacgct attaatgact ttctttttcg
     3361 cgtaaacgcc aggcgtgtaa taacggttcg gtataaccgt ttggctgttt cacgccgagg
     3421 aagattaaat cgctggcagc tttaaaagca cacgagttag cgaaattccc cgccatcgga
     3481 cgataagccg gatcgccagc attttgctga tcaaccactt tcgccatatt ctccagcgac
     3541 gcctgcacct gttctttggt cagaataccg tgacgtaacc agttggcgat atgctggctg
     3601 gagatacgca gcgttgcgcg gtcttccatc agcgccacgt tgtgaatatc cggcactttt
     3661 gaacaaccaa tcccctgctc cacccaacgc accacgtacc ccagaatccc ctgcacgttg
     3721 ttatccagct cttgttggat ctcttgcgcc gaccagttag cgttttcagc caccggaata
     3781 gtcagcagat cgtccagcag cggttcaaat tcagcattga actcggtctg ggcaatgttg
     3841 gcttgcacgc tctgtacgtt ggtttggtgg tagtgcagcg catggagcgt agcagcggtt
     3901 ggtgacggaa cccaggctgt gtttgccccg gcacgcagtt ggtcgccctt ctggctgtac
     3961 atgtctgcca tcaggtccgg cattgcccac atgcctttac cgatttgcgc tttaccgcgc
     4021 agcccacaga acagaccgga aagcacgtta ttacgttcgt aggctttgat ccacggcgtc
     4081 gatttcatct ggtttttacg cagcatcggg ccagcttcca tcaccgaatg catttcatcg
     4141 ccggtacggt cgaggaaacc ggtattgatg aacgccacgc ggttgcgcgc ctgagcgata
     4201 cagctccgca agttcagcga ggtccggcgt tcttcatcca taatgcccat tttcagggta
     4261 ttcggtacta taccgagcat tgtctcaatg cgggtaaaca gtttgttggc gaacgccact
     4321 tcctgcggac cgtgcatttt cggtttcaca atatagacgc tgccagtgcg cgagtttttc
     4381 tgcactttta aatcatagag ggcaatcgcg ccagtcatga cgccatcaag aataccttcc
     4441 gggatttcat tgccttcgct gtcccaaatc acaggaatgg tcatcaaatg acccacgttg
     4501 cggataaaca gcaacgagcg tccgtgcaga gaaatttcag agccatcggc ggcggtgtac
     4561 tgacgatcgt cattcagttt acgtacgatt tgccgaccgt ttttctccat tttctcttgc
     4621 agagtcccct gcatcaggcc cagcagattg cggtacagca ggattttatc ttccgcatca
     4681 accgccgcga ccgaatcttc gcagtcgaga atggtactga tagcagcttc gacgataaca
     4741 tcgttgatgt gcgccggatc gtctttgcca atccgcccgt tggcatcaat ttgcagttca
     4801 atatgcaggc cgttattttt cagcaaaatg caggtcggcg cagcggcatc gccacggtaa
     4861 ccgacaaact gtgctggagt acgtaacgtg gtttctttac catttttcaa ctggatgcgt
     4921 aattgtttat cgaccacctt aaacgccacc acatcctgat agctgccgtt ttccagcggt
     4981 agtgattcat cgaggaaacg tcgaacccag gcgataacct gctcaccgcg ctgcggatcg
     5041 tagccactga ccatcgcccc ttcctgcggg atgatgtcgc tgccgtataa cgcatcgtac
     5101 agtgagcccc agcgagcgtt cgccgcgttc agcgcgtagc gggcgttcat tgccggaacc
     5161 accagctgtg gccccgcctg actggtgatt tcgctgtcaa tgcccgtggt ttccaccgtc
     5221 acgcgctccg gttgcggcac caggtagccc agttcacgaa ggaaagattt ataggccgct
     5281 ttatctttta ccggccccgg attgctgcga tgccactcat caagcgccgc ctgaatgcga
     5341 tcgcgttctg ccagcaactg acgattttct ggtgccagat cgtgaacgat ctcatcaaca
     5401 ttgtgccaga atgcagcagc gtccagttcc acgcccggca aaacttctcc atccacaaaa
     5461 cgtttaaaat tggcgtcaat gcgtaaacgg ccctgggtta tggtttgact cattgtttct
     5521 ctcctcgttt tcgcttattt cgccaacacc gctgctgccg ctttcgcgac ctgcgcatcc
     5581 tgtgctccgg ttaaaccaga aacgcccacg gcaccaataa tttgcccatc aacaacaacc
     5641 ggtacgccgc cttccagcga cgtcagtaac ggcgcagtca cgaacgcggt acgtccgttg
     5701 ttcaccatct cttcatagcc cttagtttca cgacgcccca gcgcggcggt acgcgctttc
     5761 tcctgggaga tgtaagccgc aatcggcgcg caatcgtcca tgcgacttaa cgccagcaga
     5821 tgaccgccgt catcggcaac agcaatggaa acagaccagt tatttttctg cgcctcttcc
     5881 tgacctgcgg caataattgc actcgccatt tgctggctaa gaatgacttt agttttcatt
     5941 tttttattcc ttttcaaggg cttgttctac aatttcaatc cagtgacgca cagaggtacg
     6001 accggcgctc gccagatgcg tctggcaacc aatgttggcg gtgacgatca tttccggttt
     6061 gccgctttcc agcgcattca ttttgttatc ccgcagctgg cgtgccagat cgggatgcgt
     6121 taacgcatat gttcccgctg aaccgcagca cagatggctg tcgggaacgt ccgttaaggt
     6181 aaatccaaga cgaagtaaca cgttttccac ttcgccgttc agcttttgcg catgttgtag
     6241 ggtacacggg cagtggaagg ccagtttttt atcgccgcga attgccagtt tttccagcgg
     6301 ttcctcgcgc agaagttcga ctaaatcgac cgccagttca ctgacctgac gcgctttatc
     6361 ggcatataac gcatcgtttt tcagcatctg cccatactct ttgacgaacg cgccgcaacc
     6421 gctggcggtt tgcaaaattg cctcggcacc tgcttcaatc gcgggccacc aggcatcaat
     6481 attattgcgc gcccgtgcca accctttctc ctgcgcatta agatgatagt ccaccgcgcc
     6541 acaacagcct gcttcgttag ctgacatgac gctgatcccc agacgatcca gcactcgcgc
     6601 agttgccgcg ttggtgttgg gcgaaagcgt aggctgggcg cagccttcca gcattaaaac
     6661 ccgacgctta tggcgcagcg gcggacgcgg tttagctttc accgtttcag caggcagttt
     6721 tgctctgacc tgttccggta aaaacggtcg cagcaccagc cctacctgcg tcagcgcacg
     6781 gaagaccgcc ggacgcggca ctacctggcg caatccttcg cgcagcattc gctccggcag
     6841 tgggcgtttc actttctgct cgacaatatc acgcccgata tccagcaaat tgtgatagcg
     6901 cacaccagaa ggacaggtgg tttcacaatt gcggcaagtg aggcagcgat cgagatgctc
     6961 ctgtgtttta agcgtgactt cgttgccttc cagcacctgt ttaatcagat agatgcgccc
     7021 gcgcggcccg tccagttcat cgcccagaag ctgataggtt gggcaggttg cggtacaaaa
     7081 tccgcagtgg acacaggcgc gcaggatgct gtcggcttcc agcgcgcgcg cgttctgccg
     7141 catctcttcg gttaatcggg tttgcatagc ctgctcctca aagttccgcg tacatgcgac
     7201 cggggttaaa cacgccgcaa gggtcgagct gctgtttaag ctgctggtga tagcagaata
     7261 aaggagccga tagcggggca aagccaccat ctccggcact aaagcgggtc gcatgaccgc
     7321 cagcgttgcg ggcgatgcga tggatttgat tgtcatcggc tgtcgatttc agccagcgta
     7381 acgccccgcc ccagtcgatc agttgctcgc cgggtaaatc catcatcggc gcatcgctgg
     7441 gtaatgaaat gcgccataag gtacctggta acgagaagaa cggcagttgt tgttcacgca
     7501 attgctgcca gaactgaccc gcaacctctt cgccacccag cagttcacgc gctgctttta
     7561 ccgatccttc gccgccctca aggcggatcc acaacgcatt gtcgaagtaa cataagccac
     7621 taatgggtaa tggctggagt tgccactcgg cgatttcact catggcttct tgcaggctga
     7681 tttcccgacg caggctcaga gaggcacgcg gtcgcggtaa cactttcatt gagatttcag
     7741 tgagcacgcc aagacaaccg tagcttccgg ccattaaccg tgagagatcg tatccggcaa
     7801 cgtttttcat cacttcgcca ccaaaacgca gatgttttcc agtgccggta atgatgcgcg
     7861 tgccgaggac aaaatcgcgg accgaaccgc tccacgggcg acgcggcccc gccagcccgc
     7921 aggcgaccat cccgccccag gtggcttctt caccataatg cggcggctca cagggaagca
     7981 tttgcccggc gctctccagc gccgcttcaa tcgccaccag cggcgttccg gcacgagcgg
     8041 ttatcaccag ctcggtcggg tcgtaattaa caatgccgcg atgacaacgc acatccagcg
     8101 tttgcccggt gactgggcga cctaaaaagg ctttgctatt gctgccctga atcaccagcg
     8161 gcgttttatc gctaattgcc tgattcacct gctccagcag cgcctgactg taatcacact
     8221 cgcgtagcat caaaagcgct ccagttcagg aaaaggtaaa tgaccgtgat gcacatgcat
     8281 ggcaccaaat tcagcacagc ggtgtagcgt gggaatgttt ttcccagggt tcagcaaacc
     8341 atcggggtca aacgccgcct tgaccgcatg gaaggtcgtg atttcatcgc tgttgaactg
     8401 ggcgcacatt tgattgattt tttctcgccc gatgccatgt tccccactga tgctgccgcc
     8461 aacttcaacg cagagttcga ggatcttccc gcccagctct tccgcgcggg caaattcacc
     8521 aagttcgttg gcatcgaaaa ggattaacgg gtgcatgttg ccatctccgg catgaaagac
     8581 gttggcaaca cgtaaatcat attgctgcga taaacgggca atgccttcca gtacgccagg
     8641 cagggcgcga cgcgggatgg tgccatccat gcagtagtaa tccggggaga tacgtcctac
     8701 cgccgggaac gcatttttgc gaccggccca gaaacgtacg cgctctgctt cgtcctgtgc
     8761 caaacggacg tcagtcgcgc ccgctttcaa caagatgtag ttaacccgct cgcagtcttc
     8821 ctgtacgtca gactccacgc cgtccagttc gcataacaaa atcgcttcgg cgtcgacggg
     8881 ataaccggca tgaataaaat cttccgccgc gcggatcgac aggttatcca tcatctccag
     8941 cccgccgggg ataatgccat tggcgatgat gtcaccaacc gcaagtccgg ctttttctac
     9001 cgagtcaaag ctggctaaca gaacccgcgc cacgggcggc ttcggcagca gttttaccgt
     9061 cacttcggtg gtcacgccga gcataccttc cgatccggtg aacagcgcca gcaggtcaaa
     9121 accaggtgaa tccagcgcgt ccgatcccag cgtcagcgcc tcgccgtcca gcgtttgcac
     9181 ttcaattttc agcaggttat gtacggtcag accatatttc aggcagtgga cgccaccggc
     9241 attttccgcc acattgccgc caattgaaca ggcgatttgt gaggaaggat ccggcgcgta
     9301 atagagatta tgcggcgcaa cggcctggga gatcgccagg ttacatacgc ccggctgcac
     9361 gcgcgcgcgg cgaccaacgg ggttaatgtc gaggctctct ttaaagcgcg ccatcaccaa
     9421 caacacacct ttttccagcg gcagcgcgcc accagaaagc ccggtgcctg caccacgggt
     9481 caccaccggt acacgcaggc gatggcagac agccagaatc gctgtcacct gttccatttg
     9541 cttaggcaga acaaccagta atggacgcgt gcgatacgcg ctcaacccgt cacactcgta
     9601 aggaatgatc tcctcatcgg tatgcaggat ctcaagtcca gggacatgct cacgcagtgc
     9661 catcagtacc gatgtgcggt cgacatcggg taaagcgcca tcaagacgct cttcgtacaa
     9721 gatgctcatg agtaggcttc gctttgttgt gtggcagctg atttttgcgc gctgcttctg
     9781 tgaacagtta ttaagcgggc ttttcgtttt cgtctatctc tttagctacc ggtcagacca
     9841 ttttttttcc agctctgtga ccttgtcttg gttaacttaa tgttaaattg atgtaacata
     9901 atcacttgcg tgatgtgcgt gttttgcgag ttaagaacag aaaaattggt cctacctgtg
     9961 cacgaggtcc gggaatgaaa gatgaacgtc gccctatttg cgaagtggtt gcagagagta
    10021 tcgaacggtt aattatcgac ggcgtactga aggtcggtca gccgcttccc tcggaacgtc
    10081 gactgtgtga aaagctcggc ttctcacgct ccgcactgcg tgaagggctg accgtgctgc
    10141 gcgggcgcgg gattattgaa acggcgcagg gtcgcgattc tcatgtcgca cggcttaatc
    10201 gggtgcagga caccagcccg ctgatccatc tgttcagtac gcagccgcga acgctgtacg
    10261 atctgctcga cgttcgcgca ttactggagg gcgaatcggc aaggctggcg gcaacgctgg
    10321 gaacgcaggc tgattttgtt gtgataaccc gctgttatga aaaaatgctc gccgccagtg
    10381 agaaccacaa agagatttcg ctgatcgaac atgcgcagtt ggatcacgct ttccatctcg
    10441 ccatttgtca ggcttctcac aatcaggtgc tggtgtttac gctgcaatca ttgaccgatc
    10501 tgatgtttaa ttcagtgttt gccagcgtaa ataatctcta ccatcgacca cagcaaaaaa
    10561 agcagatcga tcgccagcat gcgcggatct acaacgcggt gttgcagcgg ctgccgcacg
    10621 tcgctcagcg cgcagcgcgc gatcatgtac ggaccgtgaa aaagaatctc cacgatatcg
    10681 agctggaagg ccaccatttg attcgctcgg cggtgccgct ggagatgaac aaggtgggaa
    10741 tgtgagcaga gttttaccct atccccaagg cgaggggacc gcccctgcac ttaacaggat
    10801 ctggtgttaa atctgttcat ctataccctc tcccgaatga ccgcccctgc gctaaacagg
    10861 atctggtatt aaatttgctt ttcatacaat cggtaacgct tgtacggctc cgccccaatg
    10921 cgttccagca tgttattcat gcctgtattg gtttcgagga tccattacat ctccagcgca
    10981 tcgatcttcc ggcgggcaaa cggatcgcgt aaggcttcaa tcaataacag cgcaatcacc
    11041 gggccgatgc ggctgaactg atactcgtcg cgcacgccca tcagcggtac tcgcgcagtt
    11101 cgcacaccgc tgactttcaa acgccacagc aattttgccc agccgaaggg aaagagcgat
    11161 ccgttcagat cggcaatcgc ctcgttgatg ttaggcaagc cgacaataaa cgcgcagggt
    11221 gcagaatcaa tctcagcgat gtagatcata tcgtccggca ccagatattt aagttggtcg
    11281 cccatggtcg cgaattcatg ttcggtaaac ggcacaaatc cccagttgtg ctgccagcca
    11341 gagttgaaaa tctcacgcag gatctgcatc tcttcggcaa accgctgacg attgatgcag
    11401 cgaatggtca cctttttgcg cacctgatcc atcagttttt ttagcgccgg agagaaagtg
    11461 agatcggttc gctgcatcca ccacgccagt aaatcaatgc ctttgtgata acccagttgt
    11521 tcaatatgcg cggcatacca cggtttgccg tgtggcatca tcgcacaggg tggtgtgtca
    11581 aaaccttcaa tcagtaatcc gctttcctga ttgatattca ggctgaaagg cccgctgatc
    11641 ttacttgcac cttgtgactt caaccacgct tccgctgcgc caaacaacgc ggcaaaatcc
    11701 tgcggatcat caatggcgtc aatcatgccg aaatgaccgg tatcttcacc gtaacgctca
    11761 cggtgcaagg tatctatttg cgcggtaata cgcccaacta tctgccccgc tttttttgcc
    11821 acccacgcct gccagatgat atggtccgtc cccggatttt tcgcagacaa atgctcgttg
    11881 cgttcaatga ataaaggggg tatccagttt ggatcgtcgg gataaagtga tgacggaaaa
    11941 gcgataaatg ccttaaggtc atttttatta attacttttt caattttaat catcagcaga
    12001 tcgcattcca tatctctgtg gataacaaat aattaatggt gaaaaacagg ttgcaacagc
    12061 aacgagcggc atcttaataa tcaactcaat gaatacaaac gacgaataaa tagtattaat
    12121 taatgattat gataaggtca gaggctacac agaaacgcct caatgccttt tgtcgattta
    12181 aggcgaccgg acaaggcttc ttcttaatat attcataaat taaatatcat tcataataaa
    12241 aataaaaaac tcaaactcat tatttaaatc aatgttcatt ttcacaccga tgaaaatact
    12301 atctcattta tcttttatgc ctatataatc caccgcatta ttgtcatttt atttttgata
    12361 tccaattttt tcgtggtgta tatgtctaat aaaatcttta cgcattccct acctatgcgc
    12421 tatgccgatt ttccaacgct ggttgatgct ttggactacg ccgctctgag tagagccgga
    12481 atgaattttt atgacagacg ttgccaactt gaagatcaac tggaatatca gacattaaaa
    12541 acgcgtgccg aagctggtgc gaagcggttg ttatcgctga acctgaaaaa aggcgaacgc
    12601 gtggcgctga ttgccgaaac gagtagcggg tttgtagagg ctttttttgc ctgtcagtat
    12661 gctggcttag tcgccttccc gttggcgatt ccaatgggcg tcggtcagcg ggattcctgg
    12721 agcgccaaac tctagggttt actggcaagt tgccagcccg cagccattat cactggtgat
    12781 gagtagttgc cactggtcaa tgccgcgacg cataacaacc ccgaattaca tgttttaagc
    12841 cacgcttggt ttaaggcatt accggaagcc gatgttgcgc tccagcgtcc agttccgaac
    12901 gatatcgcct acctccagta cacctccggc agcacccgtt ttccccgtgg cgtcattatc
    12961 acccatcgcg aagtgatggc taatctacgt gctataagcc acgatggcat taaattacgc
    13021 cctagcgacc gctgcgtctc ctggcagcct ttctaccatg atatgggact ggtcggcttt
    13081 ctcctgcccc ccgtcgccac gcaactttca gtagattatt tgcgcactca ggattttgcc
    13141 atgcgtcctt tgcaatggct taaattgatc agtaaaaatc gcggcaccgt ttccgttgcg
    13201 ccgccgtttg gctatgaatt gtgccaacgc cgcgtgaatg aaaaagatct cgctgaactg
    13261 gatctttcct actggcgcgt cgctggtatt ggtgctgagc cgatctccgc agaacaactc
    13321 catcaattcg ctgaatgttt ccgtcaggtt aactttgaca ataaaacttt catgccgtgc
    13381 tacggactgg cagaaaatgc gctggctgtc agcttctctg atgaagcctc cggggttgtg
    13441 gttaacgaag tggatcgcga catcctcgaa tatcagggta aagccgtcgc gccgggtgca
    13501 gagacacgcg ccgtatcgac tttcgtcaac tgcggcaaag cgttgccgga acatggtatt
    13561 gaaatccgca atgaagcagg tatgccggtc gcggaacgtg tggtaggcca tatttgcatc
    13621 tccggtccca gtctgatgag cggttacttt ggcgaccagg tttcgcaaga cgagattgcc
    13681 gcgacgggct ggttagacac cggcgacctc ggttatctgc tggacggtta tctgtatgtc
    13741 accggacgca ttaaagatct gattattatt cgtggccgta atatctggcc gcaggatatt
    13801 gaatatatag cggaacagga accggaaatt cattctggcg atgcgattgc ttttgttacc
    13861 gcccaggaaa aaatcatttt gcagatccag tgtcggatca gcgacgaaga acgtcgcggg
    13921 cagcttatcc acgcgctggc ggcacggatc caaagcgaat ttggcgtgac cgcggctatc
    13981 gatctgttgc cgccccacag tattccccga acgtcctccg gcaagcctgc ccgtgcggaa
    14041 gcgaaaaaac gttatcagaa ggcttatgct gccagtctta atgtgcagga atccctggca
    14101 tgaatcaaac tgtcgcggtg acgggcgcta ccgggtttat cggtaaatat attatcgata
    14161 acctgctcgc ccgcggcttt cacgttcgcg cattgacgcg tactgcccgc gctcacgtca
    14221 atgataatct cacctgggtg cgcggttcgc tggaagatac tcattcactt agcgagctgg
    14281 ttgccggagc cagcgtagtg gtccattgcg ccgggcaagt gcgcgggcac aaagaagaga
    14341 ttttcacccg ctgtaacgtt gacggcagcc tgcgcctgat gcaagcagca aaagagagcg
    14401 gcttttgcca acgttttctg tttatctctt cgctggcggc gcgccatccc gagctctcct
    14461 ggtatgcaaa atccaaatac gtcgccgagc aacgactggc agcaatggcg gacgaaatta
    14521 cgctgggcgt ttttcgcccg acagccgtgt atggtcccgg cgataaagag ttaaaaccgc
    14581 tgtttgactg gatgctacgc ggcctgctgc cacgacttgg tgcgccagat acacaactct
    14641 cttttcttca cgtcaccgat tttgcgcaag cagtgggtca gtggttaagc gccgaaactg
    14701 tacagacgca aacctatgaa ttatgcgatg gcgtcgccgg tggctatgac tggcaacgcg
    14761 tacgacagct tgccgccaaa gcccgttgtg gttccgtgcg aatggttgac attcctctgc
    14821 cggtactcac aggccttgcg gatatcagta ccgcgttgag tcgcctggca ggtaaagaac
    14881 ctatgctgac ccgctcgaaa attcgtgaat taacccacgc cgactggtcg gcaagtaata
    14941 accgtatttc tgaagatatc aattggtttc ccgggattag cctggaacac gcattacgca
    15001 acgggctatt ttgattgtct tgttttaaag gtgtgttaac gtttctatgg taaatcgtga
    15061 aatagtaatg gattacatcc tttcctgttt acaggatttg gtcgaaaacg gtgtggaaat
    15121 taagcccgac agcgatctcg tcaacgatct tggcctggag tcgatcaaag tgatggatct
    15181 cttaatgatg ctggaagaca gattcgatat ttccattcct attaatattc tgctgaatgt
    15241 caaaccccct gcgcaattaa tggaaactct acttccatgg ctggagaata aataatgggg
    15301 ctatacgata aatatgcgcg ccttgctggc gagcgtctgc aatttagtga caacgggtta
    15361 acaccgtttg gcacctgtat tgatgaagtg tattccgcca ccgaaggccg gatcggcaat
    15421 aaaaaagtga ttctggccgg gaccaataat tatctcggtt taacctttaa tcatgatgcc
    15481 atcagcgaag gtcaggccgc gttagcggcc caaggcaccg gcaccaccgg ttctcgtatg
    15541 gcaaacggca gttatgcgcc gcacctggcg ttagagaaag agattgccga gtttttcaat
    15601 cgccctaccg ctatcgtttt ttcaaccggt tataccgcta atttaggcgt catcagcgca
    15661 cttgccgacc ataacgccgt ggtgttactg gatgccgaca gccacgccag tatttatgac
    15721 gcctgttctc tcggtggcgc ggaaattatt cgtttccgcc ataacgatgc taaagatctg
    15781 gaacggcgca tggttcgcct cggtgaacgg gcgaaagagg cgattatcat cgttgagggc
    15841 atctacagca tgctcggtga cgttgcgccg ctggcggaaa ttgttgatat caaacgtcgt
    15901 ctcggtggtt atttgattgt cgatgaagcg cattcgtttg tcgtgttagg cgcaacgggg
    15961 cgcggactgg ctgaagctgt cggcgttgaa gatgatgtgg atattatcgt tggtactttc
    16021 agtaaaagtc tggcctcgat tggcggtttt gctgtcggtt cggaagcgat ggaagtgctg
    16081 cgctacggca gccgcccgta tatttttacc gcctctcctt ctccgtcctg cattgcgacc
    16141 gtgcgctcgt cgttaagaac tattgccaca caaccagagc tgcggcaaaa gctgatggat
    16201 aacgccaacc atctgtatga cggtctgcaa aaactgggtt atgagttaag ttcacatatt
    16261 agcccggtag tgccagtaat aatcggttcg aaagaggaag gtttacgcat ctggcgcgag
    16321 cttatctcgc cgggcgtgta tgtgaatctt atcctgccgc ctgccgcgcc tgcaggcatc
    16381 acgctgctgc gctgtagcgt aaatgctgcc cacagtcgtg agcaaatcga tgccatcatt
    16441 caggcatttg ctactctgaa gcagtaatgt gagcccgccg tggtgtttgc gccacggcgg
    16501 gtttaatgtt gtctgtcata ccaatacacc aggctcaacg ccaccaacac aaacgctacc
    16561 ggtaaaccga gcgcggtaac aggtgcgctt aacgcaaata gcaatcccag attaaccatg
    16621 atttgatagc tgatccaggt gagtaagccc acgatgacgc ctacagcgag acggctaccc
    16681 atcccaggcg aacgcggggc actaaaggta aacggcacag ccagcaaaat catcgccagg
    16741 gtcaggatgg gttgccccag tttttcccac agagcaaggc ggtattcgct gctgggttgc
    16801 ccggtttcct gcaaataatg gatgtaatga ttaagctgac ggacagaaaa agtattcccc
    16861 ggcattgata actcttcaag attcatactg gtgaaggccg attgccaggc aagattatct
    16921 aatgtttcca gcgtttcttt gccattcagc cattttttat gattcacacc atgcaggacc
    16981 cacgttttgt cgtcttcaat ggtggcactg cgtgcataaa tgtaggattc aagcgagaga
    17041 tcgtcgcgat aatgaaatat ctccacgccc accggctggc cctgctcgtt cagcgatttc
    17101 actgtcacaa attcattacc acgcctggcc cacagcatat tgccggtaat gtcatcgtcc
    17161 tcccccaacg cggtggcggt cgatttgatt tgcaacgcct gttgctgcaa tggcgacgcc
    17221 acccactcat ctatcgcgcc taacgaaaca gtccacaata tcccggcaac cagcgccacc
    17281 agtgcaatac ggaagataga aaaccccgtg ctgcgaatgg cggtaagctc actgtttttc
    17341 gataactgcc ccagtccaac aataccaccc aataacgcga taaacggcga aagttcgacc
    17401 agtgtgcgcg gtaaggtcat tagcaccacc agcaccgcct gagtccagcg ataaccgccc
    17461 gggctgacac catccagttc gttaatcagg ttgaaggtgg taaaaagcgg cagcaatagc
    17521 cctgcagcgg cggcaaaacc gagaaagaga tgacggatta aatagcgact gaaaacattc
    17581 atcgggaaaa tttctgcagc aaagagaagt cacgtgcgac cagtatcagc aggcccatta
    17641 gcatgagtcc gggcactaac cacaaaccag gaatgagggg aatcgcgccg ttagccacca
    17701 gtgtacggca gacgtcgccg ccataaaaaa tggcaataaa cagcaacgtc agtggcaata
    17761 acgttgaaaa tcgcccttgc cacggtttta cccgacttaa tgaaatggcc agcagcgcca
    17821 tcaacaatgc ggtcaggcca cggctttgtc gccattgcag ttcggcatga tcggcaggaa
    17881 aggcggagcg cgccagctcc gtgaccgatt tcgctttgcg tttgacgttc gggctttgat
    17941 ccagcggatt cagatgtaat tgcagattac ggtagatctg ttcgttgtcg tcacgcccct
    18001 gatgatcgag aagataggca gtgccgttgt gcaacataac ggtcggtttc tcgggtgatg
    18061 ggtcaaccac atcaaccgaa cgggcgcgga aaatacgggt tcgattggca gtagaagtat
    18121 aaatcagcgc gtcagtcagg cgattattat cctgatcaac cgtctgcgaa aggatcattc
    18181 gtccgttatc gttagtgtta aatttctttg cccgcaactg gcgaacatcc agctccgact
    18241 gcgactgctg ctccagttga taaatctgcg cataagccca cgtacgacca tacatcgaca
    18301 gaagggtgac aaaaatactt agcggtatcg ccagataaag aacggctttg tacaagcgtc
    18361 ccggactgcc ccccgccgca gagatagcgg taatttccga gtcggtatac atctgcccta
    18421 gcgtcacgcc aactgacaca tacagaccaa caggtaacaa catctccagt gcaatcagta
    18481 ctttgtagaa aacgatatcc agcacaacat caagagctaa tgtcccattt gccgcttcgg
    18541 tcagataacg ctgtgcggag tagctggcga aaataaagat cagaaaaccg acgataatca
    18601 gcaccagacg gcgcgtacca cgcatgatgt agtgttcaac cagcttcatc tcactaacgc
    18661 cctcaatcct ttgagttttt tattcattgc tcagtttctg cgccaacgca atgacatgat
    18721 ctacctcttc cggcgtcagg ctgccgtcct ttgcccactg aatttgcccg gctttattca
    18781 gcaccagaat ggttgaactc tgctccggca atcgccaggc aacgcgtccc tgtccattgc
    18841 cgtcaataac gaattgcgcc cacggatagc ggcgtttatt tttttcgatt ttgccaacaa
    18901 caaaatagcc agtaccaaag atggcatcat cggtgttcac aatggtggtg ggctgaaaac
    18961 gatcctgcgg aaagtttgcc gcttcaacag ctttaattaa cagtgagttc tttttcttcg
    19021 ccgacttacg cccggcgata tactgaataa tgcggacttt tcccgctaat tgtgtgctgt
    19081 tccacttttg gtagtgaatt tcgtcgtcgc tattcagaag cagctcgccg ccttcctgga
    19141 tataaacagg ggtaaccgtt ttgccagtga cgaaattgtg tgcctgcaca cccggggcta
    19201 agagcatgat aatcaacgca ataatcaaac gagatgacat cattgctccg gctatagcgg
    19261 aaaacgcgaa atcagcagct ctaagcgctg acaaatatcg ctatccagcg ccaacggtgc
    19321 ataatacgac tggcggtttt ccatatcatc cgccaccatg tcgtcgtgtt tatctcccat
    19381 caggccatta atggcggcga ggaaacggga aatctgtaac gcctctttat tttcaggatc
    19441 aagcgatctt aaggtgtcgt aataagaaac ctgctggctg atgatttttc gcacatcatg
    19501 aaattccctg ccggttagtc gcggtttggc aataatctct ttcagggtat gcatttgctt
    19561 ttgccgatag gcaataaaac tgcttacgct ttctaactgg ctatggcgta atgaatagtt
    19621 caccaacgac cagaccggag agcttaaata aatccgtaac aggttgccgt aatacgacac
    19681 aatatttttc ttaccgttac gaaagccgtc ctgaaaacgc cccagaaaaa cggtcgtttt
    19741 gccgaataaa aatccggcct gatgcttctt taaatacagc cgttgcgcaa aacgcaggtg
    19801 cttgtagcgc gctcgcatgt atttaaattg tttgcgctca tcagcagtca gctcatcgcc
    19861 tttggcttgt ttgcgaacta aacgcagtaa ttcctgacga tcaacgccat tgagccagaa
    19921 ttgcaggcat aacgcgtaat tatcatgaat gatttcggtg gtacaactta gtgcaatagt
    19981 cggcggcaaa ctaacctgtg catcaacgac atcatcttct tcaatcgcaa caaatagacg
    20041 ctcgctcgcc tcaagcggga aatattttct catcatcagg atttctctcc ggctgcgtta
    20101 taaagcaagg aatggcgctg aaaaaatgcc tttaaggttg gaattaacga gagcgccacg
    20161 tccagacatt gcccaccgaa gtaagcaaac gctgctccca gcacaccaaa cagatgacag
    20221 aacatgaaca gcaatacgat gtagccgatt gaggcgatgg tttgcgccac gagaaaagcg
    20281 cgctgttttc ccgccatcaa tagcaatgac tcctgcggga agcccagcat cgaaatcaca
    20341 attgcgccca gcatcacctg aatcaggtca tacgcttcga gatacttaac gccaaacacc
    20401 agcgaaatga gcggcttgcc gacgatcaac accgcgagcg ccaccagaat accgattccg
    20461 cccgccagta aaccggactt cacacctaac aaccacggtc tggtggtgcg cggatctaaa
    20521 cgcatcacct ctgggtaaaa acttttaccc agcaaacccg ctggcgttcc ggcggcgtcg
    20581 aagaatgtca tggcgatttt aaataaccca gcggcagcgg gtcctaacac gatccccacc
    20641 aacactgtgc tgcacgagtt acgcgccgac cagatggagt gggcaatgtt ggttgaccag
    20701 acaaaactcc acgcgccttt aatgtgtcgg gcagactcaa acagattcaa tttgaaggcg
    20761 ttatggatat ttctacggcg taattcgcgc gcggcaaacc accagtacat ggtgccgcca
    20821 accagattcg acacgtacca ggcaataaca aaacccgcaa aaccaaagtc aaaataccag
    20881 gctgcgacgc tccccgctgc gcgcagaaaa ggtttcgtcg cctgctgtac agcaatcaaa
    20941 tcaaagcgat ctaccgcacg cagaatgccg gtcggcgtgg aggaagccat tgaaggaatg
    21001 agcgtgcaat acagcgccgc cagccaaaag ctttggtcat ccagacctaa tgaatgggaa
    21061 aggaatggca gtaaggcaat gccgccgaca atcgccaccg cgccgctgac gatatccagc
    21121 gagaaggaaa atgagacgac attgcgaaat tgctgcggat tattgttggt caaggctggc
    21181 gttccgtact gaaccaccag ctgccatgtc tgaaacttaa taaaatcgct gatcgacttg
    21241 gcgtacgatt gcacaatcac cagtacacca aacatggcgg gcgtcatccc tttaccggca
    21301 cacgagagcg ccaacagccc cagcaaggcg ctctcgacat tactggagcc taaccaggcg
    21361 ctattgcgaa taatggtgcg aaacgcgcca tctgcaaacc aatgtttgat gttaaaacca
    21421 gccaattcag cctgacctta tcgttgatag taaaaaatat cccgccagcc ttaagttaaa
    21481 cttcggcggt cagaaacgat ggcaaccaga gaaaccgcct tctgtacctg ttccagcact
    21541 tcgctgtagg gcgctctgga atcaatctca agaatttttg tgccgttata gccaatcgtc
    21601 gacatgacgc cgattttgtc ctgcagctcg gcatactcat ggtcaggctt gcgggagatg
    21661 gcagtatcaa tatcaatccc caaacgaatg attaattcag gacggtattg cgccattttt
    21721 tggtataaac tccgttcgcg ctgcgccaga aacatgctga ttttcccggt cgcacgttcg
    21781 acgccgatcc ccggtccgtc ataataaaag cccgaaattt cagcctgcgg gaagcgatcg
    21841 ctgaccacca gaacgccact ttgcgccagt cgctgaacct tgcgtagatt cgccattctt
    21901 cgcagcgaga agcagtacat aatcaccgcc gcccatagcg ccggagattt ggttttcatg
    21961 ctttgggttt tcgaggattt ggccgccagt cgccgttcca gccagacgcc aaccaacggc
    22021 aatcgtttga ttttgtctcc gtcttcgccg gagagcagcc ccagatagcg gcgctcggtt
    22081 tgccagtgtt gttgcagcga tttcaccagg tcggtggtca gcgtggattt accggtgcca
    22141 tcacacccga ccaccgcaat cagccccgga atgtagttgg gctgcggcgc ggttgtacta
    22201 ttaacagttt gtgtttgtag tgcatccatt cgttaaatca tcccctatcc attttggtga
    22261 tcaggaaagc gcggcgtgaa ttgcgcgtag tgacgcttgc ataatttcat cggctggttg
    22321 ccgaccatcc agttcaagga tcttggcgcc attgaatgtc agttgcggcg taacggcgat
    22381 tttttcctgt aacgctgcca gttggtggtc aggtttacgc gcaaacgcgg tttgttcatc
    22441 aatgccaaga cgaatcaaca atacgggtaa ataagacgcc atccattggt acagcttcaa
    22501 ctcgcgctgc cttaacattt ttatccaacc gttaccgccc gtggtttttg ccaattgcgg
    22561 gccatcaaag cgaaaacccg gcacttcaac ctgcgggtag cggtcggtga tgagcagata
    22621 gccttgctgg cttttacaca acattttgcg aaacttgtac gcccgccagc aggaaagcag
    22681 atagataacc agcgcagtaa tattgccagg cggtgttgag ggcttttcgt gtacatgtgc
    22741 cgctttactt cgcagataac acccacaagg tgcgccaata acagggagct gtgaaatcca
    22801 ttcgccaatt cgcccggacg attgcccgag ataaatgtgt tctgttggca ttcttgctgc
    22861 cagttcattt accaggcttg ccgtgagggt cgatttacct gaaccatcac atcctacaat
    22921 ggcaataaca cgcactggcg tggaattaat tattgacata ttacgttgat tcacgaaaaa
    22981 cccggcagta aataatgtat tgaatattac gtggtccgtg ctcagaatat ccgttcaacc
    23041 ttattgtgac aatggttaat tgtgacattg acctgaatta cgctttcata aaaacatatt
    23101 aaccaaataa atatttttaa tggatattta aattaaagga tatattcatg cagtcaataa
    23161 cacctccatt aattgccgtt attggtagcg atggttcagg caagtcaacg gtgtgtgaac
    23221 atcttattac cgttgtcgaa aaatatggtg ctgccgaaag agttcattta ggaaaacagg
    23281 ccggaaatgt cggtcgtgca gtgacaaaat tacctttgat gggaaaatcc ttacataaaa
    23341 caattgaacg aaatcaggtg aaaacagcaa aaaaattgcc tggaccagtt ccggcgctgg
    23401 taattacagc gtttgtcgcc cgtcgcttac tgcgctttcg tcatatgctt gcctgtcgtc
    23461 gtcgcgggtt aattgttcta accgaccgtt atcctcagga ccaaattcct ggcgcttacg
    23521 atggtacggt gttcccacct aacgttgaag gtggtcgttt tgtctcatgg ctggcaagcc
    23581 aggaacgtaa agcgtttcac tggatggcga gccataagcc tgatctggtc atcaaactca
    23641 atgttgacct tgaagttgcc tgtgcacgta aacccgacca taaacgggaa tcgctggcga
    23701 ggaagattgc cataacgcca cagttaacct ttggtggtgc acaactggtt gatatcgatg
    23761 ccaatcagcc tctggaacag gtgttggttg atgcagaaaa agcgattacg gattttatga
    23821 ccgcgcgtgg ttatcactag tcaaaaatgg aaatgcccga tcgccaggac cgggcatttt
    23881 caggaaggtt aaatcaactg caatgctatc cagtacagcc caccagaaag aaaaattgcc
    23941 gccggtaaag taaacaccca cgccatcagg atgctggtta ccgttttacg ctgtaaccca
    24001 ccgccgtcca ccaccatcgt ccctgctacc gcagacgaga ggacgtgtgt tgtggagaca
    24061 ggcatcccaa tataactggc aaggccgata gacactgccg ccgtcatttg tgccgccatg
    24121 ccttgcgcat acgtcatgcc gcgcttacca atcttctcac cgatggtcat cgctacacga
    24181 cgccagccaa tcatggtgcc aatgccgagc gccagtgcta ccgccatgat gatccacacc
    24241 ggagcgtact caatggtgct taacatatcg ctgcgcagtt tcttcagcag gttctggtct
    24301 tctttactga cgcctggcag tttcgccagc ttcgcggagg tatcagagat gcacagcata
    24361 atgcggcgca gctggctgcg ctgactcacg cttaacggct cgtaactttc catattgcct
    24421 ggcagcatcg ttttaacgcg cgcaatcgca tcaaaggtat ttgccggatg acagtgaaac
    24481 tctgttactt gcgtgccatc agtcgatgct gcaggcaatg gaggttccat ggcaatcaac
    24541 ttctgcggca gttcaggatg ctgttgcagg tagtgttcga agttggtaac ggcatcgcgg
    24601 gtacgggtaa tttcatagcc ggacgcattc atattgacga cgaagccagc aggggcaatc
    24661 cccaccagta ccagcattac caagccgatc cctttctgcc cgtcgttcgc gccgtgcgaa
    24721 aacgccacgc ctgcagcgga aacaatcagc gcaatacgcg tccagaatgg cggtttacgt
    24781 ttgccctttt tctttttgcg atcttccgga atgcggtgaa tacggtcacg ctttttcgtc
    24841 ccactccaga aacggcgcag caggaaaatc agaccacccg caatgaccag gccgacgata
    24901 ggggaaacaa tcagcgagga gaaaattttg gttacttcac gcaggtttaa cgcatccatc
    24961 accgatgagc cggttaacag cgcgttggtt aaaccgatgc cgataatcgc accaatcaag
    25021 gtgtgcgaac tggaggccgg taaaccaaag aaccacgttc ccaggttcca gataatcgcc
    25081 gccagcagca tggaaaagac catcgccaga ccgtgagttg accccatatt cagcaacaaa
    25141 tcggttggta acatatggac aatggcatag gcaacgctaa gtccgcccaa taacacgcca
    25201 aaaaagttaa aaaatgccgc catcaccaca gcaagttgtg gctgcatggc acgagtataa
    25261 ataacggttg ccaccgcatt cgccgtgtca tgaaaaccat tgattgcttc gtagaacaac
    25321 acaaatgcca gagcaagtaa taacaaaagc cccgtatata tatcaaggcc aacaaataaa
    25381 tttagcatat attagattac gccattttga atttacgaac ggacgcatta tcagtgactt
    25441 taacggcatg ggcaaagtga aatatcattt tttaacgata aaaagagtgt tatttcttgt
    25501 gtattagaca ataagcattc attcaaaacc tttagaaata atcaaaacac aggtattcca
    25561 ctggttaata tattcacata tgaaatgaat aaatatttga atatataaat attgaatatt
    25621 ttgattaatc cccgtactaa ttattcttca taatcagtac ggggttgcta caacatgtat
    25681 tactttttca ccacaattaa cggttcaata tcactctctt ttttgatgac cagtgattca
    25741 tcaccgcgca aacacgtccc accgtagttg ccgccaacgg tgaaggtaca tacctgaatg
    25801 tatttaccgt ccactttcgg caaacaccac agttgctgat agatgttttt ctgctcggca
    25861 aatttaccgc tggttttgtc cagcacctct tcatgatggc cgaccaggtc gatattgcta
    25921 ccacagcgac cggcgatcgg tttcactgcg taacctgttt tcaccaattc atcattaaca
    25981 gtgaaatcgg tatccagcag gtaacgatgg tgcgggaaca gcgaccagag gatcggcaga
    26041 atcgctttgt tgccggggat caccgtccac agcggctcaa agaccagcac ttccgggcgc
    26101 agcaatacgt cgataagacg cacttcgttt tgcggatgac cggtacggat tggcaccgca
    26161 gcaaactcac ggtcgctaac ttcacgaatc tgatcaaacg cggtttccca cgcccaggtt
    26221 ttccacacgc agttaaccag tcgcccttcc ccatcaatca gttgcccggc agcatcccag
    26281 cccagttcat ccagcccgcg caagatacgc gtttcaaagc ccgcctggtg cagcgcctgc
    26341 tccataaact gcgcgtgata gttttcctcg atatctttgt cctgcatgat atggacaaac
    26401 ggacgtgcac gactgtgttt ccaggcacca gccagttcgt taatcagccc ttccgccgga
    26461 ttgaagccgt tgcctttata gccctgctcc gcccaacgtt cgaggatcaa gcccgcttcg
    26521 gtatgacagg aggcggagtc ggcgttgtat tcgtaaacct tcaggccacg ctcatccatg
    26581 cagaaatcca tacgaccagt gatcatatgg tgacggcgac gctgccagga gagacgcaaa
    26641 cgtggccaga ggattttcgg aatgtcgaac agcgccagca ggttgtcatc tttcagcacc
    26701 ttgtcggttg cgtgaagata catcagatgc agctcgttgg tggctttaat tagctcctgc
    26761 tcggcactct cggtaatggt gtagtaatga tacggatcct gattgatcac ctgaccgttg
    26821 gcctgcacat aggcgttttg tagcggatct ttttcatcca gccatttacc gtcaaactgg
    26881 cctttgtttt ccagacgcgc tccactgatt ttcagcagct cgcctgcaat ttccggctgc
    26941 ggtaagctgt attcagtatc ttccgtctgg atcatccagc ccagaatggt ggtgtcatca
    27001 aaagtgtctt taagggtata gcagccgttt tcgaccacca tctccagctc gcgcgtccac
    27061 tgttgccctt gcggcaacgg ggaatgaatc acgttctgtt ccgcaatacg gactttgttg
    27121 ccatgcaatt gggtaatgat ggcgacatgg ccagtgtctt taaattcacc gcctttatcc
    27181 cagataagaa gcgcacccgc gaccggcgca cgcggcgagc cgttaggaaa tgcctgcaat
    27241 ggcaggatgt tgtcattaac cacttctcgc aggaagcgca gcgagaaaat ctcccacgcc
    27301 atacccacgt cagtaaagac cacaccgtaa ttcagaaaga gaaaacggcg agcaaattca
    27361 acgcattgcc acttgtggcc catatattcg tcgtcgatat agctacggaa tacggcgtca
    27421 tcttcgtatt cctgcggatc gagagaactg taatctgaag agtagattgc taccccgcct
    27481 ggggcgtagc ccaataatgt cccgaacggg gcatcctggc tggtcgttcc tttgctcatc
    27541 actttacctt taacaataca acctaagcag ttggcgtaat tgttgtgctc tgattacctg
    27601 ctcagcgaca ttaaccggac agaggtcaga ctaatcatac acctcatcgc agcggctggc
    27661 gcaaagggtt aaaaaattca cattctgtac agcaagtgac ctgctacact gcttcaacac
    27721 taccactcag aaggcaactc actatgacag acaatactta tcagcccgcg aaagtctgga
    27781 cgtgggataa atccgctggc ggcgcgttcg ccaatatcaa tcgcccgttt tctggtccga
    27841 cgcatgaaaa aacgctgccc gttggcaaac atccattgca actttattcg ctgggaacgc
    27901 cgaacggtca gaaagtaacg attatgcttg aggagctact ggcgctgggc gttactggtg
    27961 cagagtacga cgcctggctg attcgtattg gcgatggcga tcaattctcc agcggctttg
    28021 tcgaagtgaa cccaaactcg aagatcccgg cgctgcgcga tcatacgcat aatccgccga
    28081 tccgcgtgtt tgaatctggt tcgatcctgc tttatctggc ggagaaattt ggctacttcc
    28141 tgccgcagga tctggcaaaa cggactgaaa cgttgaactg gctgttctgg ttacagggtg
    28201 ccgcgccgtt cctcggcggt ggttttggtc acttttacca ttacgcgccg gtgaaaattg
    28261 agtacgccat caaccgcttt accatggaag ccaagcgcct gctcgacgtg ctggataagc
    28321 aactggcgca gcataagttt gttgcgggcg atgagtacac cattgcggat atggcgattt
    28381 ggccgtggtt tggcaacgtg gtgctcggcg gcgtgtatga tgccgcagag tttctggatg
    28441 cgggaagtta taaacatgtg caacgctggg cgaaagaagt gggcgaacgt ccggcagtga
    28501 aacgtgggcg tattgttaac cgcaccaacg ggccgctgaa cgagcagttg catgagcgcc
    28561 atgacgccag tgattttgag acaaatacag aagataagcg tcaggggtaa gggttggtgt
    28621 tcgtcgcagc aagccatcca ggccggataa ggcgttcacg ccgcatccgg caatcgtgca
    28681 caatgcctga tgcgacgctg ccgcgtctta tcaggcctac aaaagctaat catcacgcgc
    28741 tggtaatgtc gtaatccatt tggcgcagtg cgtctaatgt ggctttggct tcatcttcgt
    28801 cgatgatgct catggcaaat ccgacgtgta ccagcaccca ctggcccagc agatcggcag
    28861 ggttaccttc gcaaatcagg gcgatattca cgtcgcgctt gataccacat acttcaacct
    28921 gcgcaagctg gtgaatatct tcaccgacag ccagcacctg gcctggaacg ccaatacaca
    28981 ttgttaactc cggttatttc gattgccgga caaacacgaa aaagaacctt tagcgggtgt
    29041 tccctctcac cctaaccctc tccccggagg ggcgaggggc ccgtccgtgc gctttttagg
    29101 cgttgtcctc agtcttattc aacttcaata cttttgacga tcagcgaatc gccggtatcg
    29161 acacgcaacc gctcgccgtg acagagcgga cactgcgcat cgtgctgatg aatctccacc
    29221 acctggctgc aatcccagca ccaggcctgg gcgggtttat agacgatatg taaatcgcac
    29281 ccttgcgcca ccgttccgtg gcagacaatt tcaaaactaa aacggacggc gctctcctca
    29341 acgcaggaga gcgcgccaat ttccagccac acggcggtga cgcgcttaac atcgtgctgc
    29401 tccgcctgcc gttggataat ttcaacggcg ctctggcaaa gcgacaactc atgcattttc
    29461 gcccctcctg cggccaaaca gcagggcgcg acgccctgcg tgtggtacat ccggattggt
    29521 gactggcaac gaaaggatca ttcgtgcgca gtcatccgtc aggcgttggc cttcttcaat
    29581 cgacatgctg tgcgaaagcg gcgacatcag cgaacaggag aggtattgcg aaacaccgtc
    29641 cagttcgcca acggtaaagg tcatggtgcc atacggcagt tgcagaccaa ttttttcact
    29701 cactttgcgc agcggccaga gttgatccgg gccggggaag ataactgcac tcagcatcca
    29761 cggggtgatc acacaccccg tccactgacc ttcgaacagc gtaaaatcag aaacatacac
    29821 cggcattgaa ggatgcagaa aagagagatc gtgcatcgaa cgccgggcaa tttcttcaaa
    29881 cgctgcctgt acttgcgcct tcggggaggt ctggaaacct gctatctctt cagtcatgaa
    29941 tcgcctcccg tgggatggct tccacgcctg attcgcgcag cgcagccaga accagctcaa
    30001 gcgcaggttc aatcattgct tcaaccgtcg gcgttaagcc gatgtgtggc tccagcgatt
    30061 ccgggatcac gccgaccagg gtcagctttt tcggaaactc accggtgaag cgcagggccg
    30121 acaatacgtc ggccaggcca agctgatgcg gagagatttt gttggtaaac aacgccggaa
    30181 cttcttcatc ccgcaggatc atcatcgttc ccggcgtgtt ctttttcgac acaatggcat
    30241 ccgcaataat cagatgatcg cgatttgcca tgtcgctaag cagctccatt cccgccgtgc
    30301 cgccatcgag gatctcaaca taatccggca gaatgtatcg ttgctctaac gcttcgacaa
    30361 tccgcacgcc gatggcttca tcggtcagca aaatattgcc gacccctaag actaaaatac
    30421 gcattacaga accttcactg aaaccacttc gttgccgtcg gcatccacta cgtgtaccgc
    30481 acaggccatg cacgggtcga aggagtgaat ggtacgcacc acttccagcg gtttattcgg
    30541 atcggcaacc ggtgtaccca ccagcgactg ctcgtaagga ccgacgtcat cattgaagtt
    30601 acgcggacca gagttccagg ttgatggaac aaccgcctgg tagttgctga tgataccgtc
    30661 tttaataacc atccagtgag agagcatacc gcgcggcgct tcgaggaagc caacaccttt
    30721 gaactcaccc gttgccggaa tgttcggctt cacaaaggtg gtgtgatcgc ctttgccgat
    30781 attggtgatc agtgcactgt attggttttg caggatatcc tgcaattcac agcagtgaac
    30841 ggtacgacca ataatacggc ccagcgtgga gtgcagctgt gccacttcca gcgtgttgcc
    30901 agtcagtttc tgataaatcg caacgatttc attcagtttg ttctgggtag attcgcgacc
    30961 tgccgccagt ttcaccagca tattagccag cggacccact tctaccgttt tgccgtagaa
    31021 agtcggtgat ttcacccagg aatatttccc gtcgtcagac caaccatcat aagccggaat
    31081 ggtggtacct tcccacggcg cctgtggcgc ttcgtcttta taccaggagt gcttcgcgct
    31141 ttcctgaatg cctttgatca ggtattcatc ggaatgagaa gtgatcggac gatacgagga
    31201 cagatccgca ttctcaatgt agccgcccgg gaacaggaag ctgccgtttt tgctgtcggt
    31261 cgggaattcc ggcacgctca ggtagttcac cgcaccttta ccgcgcgtca gccattccgg
    31321 gtagaacgcg gcgataaccg cggtatcaac cttataaacc tgctcaacaa agtcgctcag
    31381 tttgtcgatg aaagacttga tgtacatcag gcgctcaagg ttcaacacgc ccaacccgtc
    31441 gaggttgatt gggttcgcga caccactcac tgccaggttc tgaatgtgcg gcgttttacc
    31501 gcccagcagc gccacgacgc ggttagcgtc acgctggcac tccagcgcct gcaggtagtg
    31561 cgctaccgca atcaggttca cttccggcgg cagtttcatc gccgggtgac cccagtagcc
    31621 attggcgaaa atacccaact gaccgctggc aaccagatct ttgatcttgt tctgaacttt
    31681 agtgaactct tccggactgt tcagatgcca ggtcgaaacg cctttcagca tttcggaggc
    31741 tttggttggg tcagcttgca gtgcagaagt gatgtccacc cagtccagcg ccgaaagctg
    31801 atagaaatga acgatatggt catgcgtggt gtgcgcagcc agaatgatgt tacggatgta
    31861 ttgcgcgtta accggaacgt cgatattcag cgcactttct gccgcacgaa cggaagacag
    31921 cgcgtgagtg gtagtacata cgccacagat acgttgcaca atcatccatg catcgcgcgg
    31981 atcgcggttt ttcacgatct cttccatgcc gcgccacatg gtaccggaag cccatgcttt
    32041 cgaaacgacg ccattttcga tttcgcaatc gatgcgtaaa tgcccctcaa tacgggttac
    32101 cggatcaata gtaattctct ggctcatgct ttgctcgcct catgacgatt atgatcgttt
    32161 tgttttaaag gaggaagtat cggcagtaga cgaatgagta cgatgtaagc gcaaatctca
    32221 atagccacaa aaccaataga aatcaacagt tcttcccagg tcgggaagta ggcgtaaccg
    32281 ccgcccgggt tgaatgccac caacgaatag gtcagacgcc aggttgcaca acctaacagt
    32341 gcgctcagtg ctgacaggaa cagcatgcgg gaatcattac gcagcttcgc cacacgcaga
    32401 acgaccagcg ggaagagcat cagcaggact tcaatccaga acatcacgga gtagaagtca
    32461 ccggcaaacg ctaacgacag cttgtcgcga tagataagct cgccaaagcg cagcccgatg
    32521 aaaatcgcca gcaacacact gatggtgttg gtcagcttga cgaacaggct cttttcatcc
    32581 ggaccgttgc cacgcagacc cgcctgcacc agtgaacctt caaagatgac aatcgagaag
    32641 cccatgatga acgccgtcag cagcgagaac agcggcaaca tttcatagct ctgccagagc
    32701 ggatgcacct tgtagcccgc cgagatcatc agcgacccca ttgaagactg gtgcatggtc
    32761 ggcagcagcg caccgagcgc gatgatgaag aacatcacct tgttcagacg ttgtagcgac
    32821 accttccagc ccagacgttc aaacagtgcc ggagcaaact ccagagccat cacgccgatg
    32881 tagatggtca tacagaccgc cgtctcgaac agtaccgagt tcacgttgaa gtgacccgga
    32941 atgtagaagt acggcaggtt ccagtagcga cccacgtcga tagtgatcga caagccaccc
    33001 agtgagtaac caaacagact cgccaacagc gccggacgca ccagcggatg gtattgccca
    33061 cggttaaaga cgtataccgc ccacgccagc gcccagccgc cacaggcaaa gccggtgcca
    33121 atcagcaggt caaacgcgat ccacacgccc cacgggaagc cgccgttcag gtcagagaca
    33181 gagcccagac cgaacaccag acgcttcaca atcaggagca tacagatgac gattaacggt
    33241 ccaaaaatca tgaccggttt actgatgatt ttgccaccca gcggttgtgg atcatgactc
    33301 atgatcgtct cctccgtcgt gatggtcgtt tttggtgttg cgacgaacca gcacggttaa
    33361 gcccgccagc acagccagtg gtagcatcat gcctttatac agggtgtgtt gaatattttc
    33421 ggaacgcgca ccggtagaaa gatcgtccag tttcggcagg tcgagatttt cataaggcac
    33481 acccgtcagt accagaacct gagtaccgcc gccctctttc tcgccgtaca gatgcggata
    33541 atatttcggc accgtatgca ggtaagtgtc gccagatttc agcgtctgac gcggatagtg
    33601 gtattcgctg ccaggcttca gcgccagacg ttttttcgcc tccgccatca gctcttcacg
    33661 cgtaccgaaa atcaccgcgc ccgccgggca cacttctacg cagccaggca gaccgccttt
    33721 atcgagacgt tccacacctt tctggttgca cagctcgcac ttatgcagcg caccaaacgg
    33781 gttgttgtag tcgtacttcg gcacgttgta cggacaggcg accatgcagt aacggcaacc
    33841 ggtgcacacg tctttgtcgt aatggacaat gccggttttc ggatcttttt tcagtgcaga
    33901 gaccgggcac acagagacac agttcggatc gacgcagtgc atacactgtt tcttaatgta
    33961 cgcgtagccg ttctcctcct ggtctttgtt gacccctgtg ccgctggtcc acacctgaat
    34021 gatgttattg gtatacggcg acagtttgtc gttgttcgac caggtctgtt ccccttgcgg
    34081 gttacgttca gggaagttga tatcctgaca cttggtgacg caagcctggc agcctacgca
    34141 caaggtcgag tcgtacaaca tccccagcga tcccggaatt ggcgggcggt tttcagcagc
    34201 cgcatgactg acagacggca gcgcgcccgt cagcaatgcc ccgcaggagg ctgctttaat
    34261 aaaattacgt ctgttcacgg ttattctccc cgtgagtcag cgttatcttt cttttgctga
    34321 cgacccagtt cacgcaccgc catcacgctg acaccggcaa ccagcccaac cacaccgccg
    34381 agcaaaccaa tagcgcctgc agagacgttg ccgccctctt tagcgttaac atccggtttc
    34441 tgtgaacgcg gagtctgatt ttcgacgttg gcaagctgat ggatgccttt atggaagccg
    34501 ataccttttt cgttacagcc atagcacggg tgaccaatcg ccaccggcca cacaccgcca
    34561 acatcgcaga attgcagcgt tgagcagttg ccgtaagttt ctggcccttt acagccgagg
    34621 tggtacaggc accagccttc gcggtggcct tcatcaccga actctttggc aaaacgacca
    34681 gcatcgaagt gcgggcgacg ttcgcagtgt tcgtgaatca gacggccata ggcgaaggtc
    34741 ggacggtttt tgtcatccag tttcggcggt ttgccgtaag tgatgatgtg cgcaacggtc
    34801 gcgaggaagt tgtgcgggtt cggcgggcag cccggaatat tgataacggt tttgcctggc
    34861 agaacttctt gcaagctgac tgcgccagtt gggttaactc cagctgcggc aacaccgccc
    34921 cacgcggagc aggaaccaat agcgataatg gctgctgcgc cttccgccgc tttgcggatg
    34981 tgatccacaa tcggctcacc ggcaaccatg caataaatac cgttatcttt taatgggatg
    35041 gaaccatcca ccactaacac atactgccct ttgtacttct cgagagcgtt atgtttgttc
    35101 tcttcgacct gatgaccgaa ggcggcggaa agcacttcgt gatattccag agagatagtc
    35161 tccagcacga ggttttctac cgttggatgc gttgcacgaa gcagagattc cgtacaaccg
    35221 gtgcactcct gcgcgccaat ccagataact ggcggacgct gcgggttagt aaccgattcg
    35281 gccatctctg cagcggcttt gctacttaac cccatggtgg cggctaatgc tgcacaaagc
    35341 ttcatgaaat cacgacggtt aatgccgtga gaatggatga gggtgttatc tccagtcatt
    35401 tatagttatt ccgttgcgag gacctggcat atattttgcc tcaatcgcaa aatcaataat
    35461 gcgatcgatg cgccatttac cacacattta ttatggttat cgatatcatg atactgcggc
    35521 ggggcgctaa aacgaaggga agaagcatta gtgtaattaa ttaaagcaag ataatacgta
    35581 tgtttgatca attttcgagg cgataaccgg ccataaaaaa gcgcaacgta gaacaggaat
    35641 tattctcacg aaagaaatcc attaccatct ctttatccag tccatagcgg cgcgtcagaa
    35701 tacccgcttc ccaggcgcta agctggcgat ctccggtctt ttcgtacatc cggtgtgagt
    35761 agcctaatac aaaaccgcgt ttataatcgg cacagaattt tgtcacatta accgcactgt
    35821 cggcatgcgt tgcttttaat cccgccatta agcctttacc aaaatggtta ttcatcgttc
    35881 accccattcg ttatatatcg ccatatatag cgatatttta ggcaaagaac gacttttgcg
    35941 cacgctattt tttcatactt gtttaagacg taatcccgta agagataaac ctatcaggag
    36001 agatatttat cgaaccattt cagtgtcctt tgccaggcaa gatcggcggc agatttgtca
    36061 taacggggcg tggaatcatt atggaatccg tgattaaccc ccggatagat ataagcctca
    36121 taaaccttat tattgtcttt caacgccgcc tcgtaaggag gccagccctc gttgattcgg
    36181 gtgtccagtt ccgcgaagtg gagtagtaaa ggcgcttcaa tcttcgccac atcggcagtg
    36241 ggtgcctgac gaccataaaa cggcaccgcg caggccagtt ccggatacgc gacagccgcc
    36301 gcgttcgata cgccaccgcc atagcaaaat ccggtaatac ccactttacc tgtcgcttgc
    36361 ggatagcgtt gcataaactc aattgcggca aagaaatcat tcatcagttt ggttggatca
    36421 acctgctgtt gcagctcacg acctttatca tcatttcccg gataacctcc aacggaactt
    36481 aatccgtcag gtgccagggc gatatacccc gccttcgcca ctcgccgtgc cacatcttcg
    36541 atatacggat tcagtccacg attctcatgc accaccccca cggctggcgt tttgccgctc
    36601 atttttgcgg gtttcaccag ataaccccgt acctcgccgt gaccatttgg cgaaggatac
    36661 gtgatgtact cagcaacaat ctccgggtcg gtaaactcta cctgagtcgc cagcgcataa
    36721 tttggcttga gcaaatcaaa caacgctaat gccgtcatcc cgcccaccgc atacttcgcc
    36781 gcaaggttga ggaactcacg tttcgaaatt ttcccgtgaa cgtaatagtc gtagtaatcc
    36841 aacaactctt gtgggaaatc tttggcggtt aaacgcggca tcgttgcatt cctcaactgg
    36901 tgttttttta agcaaagcat aagcgcgtgt ttttgcccag tttttcgtca ctctgtgagc
    36961 cagactacgg gatacgcgct ggcgaatcgc taaactagaa acattgtttc gaaattgaac
    37021 ggtggaaagg agaggtcatg gtctggttag cgaatcccga acgttacggg cagatgcagt
    37081 accgctattg cggaaaaagt ggtttacgcc tgcccgcgtt atcgctcggt ttatggcaca
    37141 atttcggtca cgttaacgcg ctggaatcac agcgtgcaat cctgcgtaaa gcgtttgatt
    37201 taggcattac gcactttgat ttagccaaca attacgggcc gcctccagga agcgcagaag
    37261 agaactttgg tcgcctgctg cgcgaggatt ttgctgctta tcgcgatgaa ctgattatct
    37321 ctaccaaggc tggctacgat atgtggcccg gcccgtacgg ctctggcggt tcacgtaaat
    37381 acctgctcgc cagcctcgac caaagcctga agcgtatggg gcttgagtat gtcgatatct
    37441 tttactctca tcgcgtcgat gaaaatacgc cgatggaaga aaccgcctct gcgctggctc
    37501 atgcggtaca aagcggtaag gcgctgtatg tcgggatctc ctcttactcg ccagagcgga
    37561 cgcaaaaaat ggtcgagttg ctgcacgagt ggaaaattcc gcttttaatt catcaacctt
    37621 cgtacaattt actgaaccgc tgggtggata aaagcggcct gctggatacc ctgcaaaata
    37681 acggcgtggg ctgtattgcc tttactcctc tggctcaggg attgctgacc ggaaaatatc
    37741 tcaacggcat tccgcaagat tcacggatgc atcgtgaagg gaataaaatt cgtggtctga
    37801 cgccgaaaat gcttaccgaa gccaacctca acagcctgcg cttattgaat gaaatggcac
    37861 agcagcgtgg acaatcaatg gcgcaaatgg cgttaagctg gttgctgaaa gatgatcgcg
    37921 tgacgtcggt attgattggt gccagccgcg cggagcaact agaggagaac gtgcaggcgc
    37981 tgaataatct gacatttagc accgaggagc tggcgcagat tgatcagcat atcgccgatg
    38041 gcgaactgaa tctgtggcag gcgtcgtccg ataaatgacc tgttaataac gggcgcgcga
    38101 accgcgcccg aatcagatca gtgattatga cgagtcagtc ggtcaagata gcccatcaca
    38161 aatgcagaaa gcacaaacgt cagatggata atgacgtacc acatcagttt gttatccggg
    38221 acatttttcg catccataaa gacgcgcagt aagtgaatgg aagaaattgc cacaatcgac
    38281 gctgctactt tgtttttcag cgacgttgcg tccattttcc ccagccagtt cagcttctct
    38341 ttgttctcgg agatatccag ctgtgagacg aaattctcat aaccggaaaa catcaccatc
    38401 accagcaaac cgccaaccag cgtcatatcc accagcgaca gcaacacgag gatcaaatct
    38461 gattccgcca tcgagaagat attcggcagt acgtgaataa tctcctggaa gaacttcagc
    38521 gccagggcaa ctaacgccag cgaaaggcca aagtacacgg gggcaagcag ccagcgagaa
    38581 gcatacattg cattttcaaa aaaacgttcc atacattcct gtcgtgtagt taaaacgggc
    38641 aacagtatat cgcaattgag taaacagcac gcaacagcaa atcaacatat acgttaaacc
    38701 tttgtcgcat aattctgagg tttagggatc ttcctggttc ccttatcaac gcatcgagag
    38761 cgtctaccct taaaggacgt tgaacctgaa gggagaaaaa cgatgtctca tttaaaatga
    38821 cccgaccacg cagtattaca ctggtgaata tcccaaacag aaacaaccga cgccaggcat
    38881 ccaggcgaag atgacaccga taccggattg cggcgagaaa acctatgttg gtagcgctcg
    38941 cctgaaagat cgtaaagcac tggtgacagg gggcgattcc ggaataggtc gcgctgccgc
    39001 catcgcttac gcgcgagaag gggctgacgt ggcgatcagt tatcttcccg tggaagaaga
    39061 agacgctcag gatgtgaaaa agatcattga agaatgcgga cgcaaagccg ttctgctgcc
    39121 aggcgattta agcgatgaga aattcgcccg ttcgctggtt cacgaagcgc acaaggcgtt
    39181 aggcgggctg gatattatgg cgctggtcgc cgggaaacag gttgccattc cggatattgc
    39241 agacctcacc agcgaacagt ttcaaaagac ctttgccatt aacgttttcg cgctgttctg
    39301 gctaacccag gaagcgatcc ccctgctacc gaaaggtgca agtatcatca ccacttcgtc
    39361 aatccaggca taccagccaa gtccgcattt actggactat gcggctacga aggcggcgat
    39421 tctgaactac agccgtggcc tggcaaaaca ggtcgcggag aaaggtattc gggtgaatat
    39481 tgtcgcgcca ggccctatct ggacggcact gcaaatttcc ggcggacaaa cgcaggataa
    39541 gatcccgcag tttggtcaga aaacgccgat gaagcgtgcg gggcaaccgg cggaactggc
    39601 ccctgtatat gtttatctgg caagtcagga gtcgagctac gtcaccgcag aagtgcacgg
    39661 cgtgtgcggc ggcgagcatt taggttaaaa aaatgcccgg ttgtgaaaag caaccgggca
    39721 tcattgtgaa ttcactctta cctgatgtgg cgaaaacgcc ttatccgcct acaaacgcat
    39781 gaagattcaa catgttgcaa tagattttgt aggcctgata agcgtagcgc atcaggcatt
    39841 gagcacctaa tgccggatgc gtcgtgaacg ccttatccgg cctacaaacg cacgaagatt
    39901 caacatgttg caatagattt tgtaggcctg atgagcgtag cgtatcaggc aattctactt
    39961 tacttcgctt tggcggtttc ttcgccgacc agacctatct tcaggtaacc cgcctgatgc
    40021 agcgtatcca ttaccttcat caacgtctcg taatcgacgg ttttgtccgc tcggaagaag
    40081 atggtggtgt ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat tgtttcatcg
    40141 gtgaccggat cgttaccgat aaacatcgag ttgtctgcct tcaccgacag ataaaccggt
    40201 ttttccgggc gcggctgcgg cgtgctggta gaagcaggca agttcacctt cacatctacc
    40261 gtcgctaacg gtgccgccac cataaagata atcagcagaa ccaacatcac gtcgataaac
    40321 ggcgtcacgt tgatatcatg catttcgccg ttatcgtcga ggttttcgtt aagatgcatt
    40381 gccatcggac attatcctgc gcgtaatttt tgtgcgacac gaaccggatg cgcagcggcg
    40441 ctggcttcca gatccaggtc acggctttgc agcaacaata cctgcgctgc aacatcaccc
    40501 agcatcgctt taaagccgcc aatctggcgt gcaaatacgt tatagataac gaccgcagga
    40561 atcgctgcca cgaggccgat tgccgttgct aacagagctt ctgcgatacc cggcgcaacg
    40621 actgccaggt tagtggtctg cgtttgcgcg ataccaataa agctgttcat gatgccccag
    40681 accgtaccaa acagaccaac gaacggagaa atcgcgccga tggttgccag atagccgtta
    40741 ccgcggccca tttgacgacc cactgcggcg acccgacgct ccagacggaa gctggtacgt
    40801 tctttaatac cttcgttatc gtcgctgcct tctgacagtt ccagctcatt ctgcgcttca
    40861 ttgagcaaat gcaggcttaa gcttttgcta ccaaaatcag cggcgatatc gttggcctgg
    40921 tttaaggaac gtgcttcagc cagcagttgc tgctcgcgct taaggcgacg cttctgattg
    40981 aagaactcta cgctcttact gaagaagatt gcccaggtga ctacggaggc caaaataagc
    41041 ccaatcatca cgcacttaac gacaatatcg gcgtgctgat acatacccca gacggaaagg
    41101 tccgtctgca ttaaattatt acccactgtg tatctccagg acgcaagtca caaaatctgc
    41161 gcataataat atcaaaacga cgtcgaattg atagtcgttc tcattactat ttgcatactg
    41221 ccgtaccttt gctttctttt ccttgcgttt acgcagtaaa aaagtcacca gcacgccatt
    41281 tgcgaaaatt ttctgcttta tgccaattct tcaggatgcg cccgcgaata ttcatgctag
    41341 tttagacatc cagacgtata aaaacaggaa tcccgacatg gcggacaaaa agcttgatac
    41401 tcaactggtg aatgcaggac gcagcaaaaa atacactctt ggcgcggtaa atagcgtgat
    41461 tcagcgcgct tcttcgctgg tatttgagag tatggaagcc aaaaagcacg cgacgcgcaa
    41521 tcgcgccaat ggcgagttgt tctatggacg gcgcggaacg ttaacccatt tttccttaca
    41581 acaagcgatg tgtgaactgg aaggtggcgc aggctgcgcg ctatttccct gcggggcggc
    41641 agcggttgct aattcgattc ttgcttttgt cgaacagggc gatcatgtgt tgatgaccaa
    41701 caccgcctat gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac tgggcgtaac
    41761 gacatcgtgg tttgatccgc tgattggtgc cgatatcgtt aagcatctgc agccaaacac
    41821 taaaatcgtg tttctggaat cgccgggttc catcactatg gaagtccacg acgttccggc
    41881 gattgttgcc gccgtgcgca gtgtggtgcc ggatgccatc attatgatcg acaacacctg
    41941 ggcagccggt gtgctgttta aggcgctgga ttttggcatc gatgtttcta ttcaggccgc
    42001 caccaaatat ctggttgggc attcagatgc gatgattggc actgccgtgt gcaatgcccg
    42061 ttgctgggag cagctacggg aaaatgccta tctgatgggc cagatggtcg atgccgatac
    42121 cgcctatata accagccgtg gcctgcgcac attaggtgtg cgtttgcgtc aacatcatga
    42181 aagcagtctg aaagtggccg aatggctggc agaacatccg caagttgcgc gagttaacca
    42241 ccctgccctg cctggcagta aaggacacga attctggaaa cgagacttta caggcagcag
    42301 cgggctattt tcctttgtgc ttaagaaaaa actcaatgat gaagagctgg cgaactatct
    42361 ggataacttc agtttattca gcatggccta ctcgtggggc gggtatgaat cgttgatcct
    42421 ggcaaatcaa ccagaacata tcgccgccat tcgcccacaa ggcgagatcg attttagcgg
    42481 gaccttgatt cgcctgcata ttggtctgga agatgtcgac gatctgattg ccgatctgga
    42541 cgccggtttt gcacggattg tataacattg ccacttttgg acaattttgc agacatttta
    42601 ttgtgaaaag tcttaaattg ttgcgtccgg gatcaaggcg tcccggacga ttcaggagta
    42661 caataggcag ataaaagctt aaacgctgtt ccacaggaaa gtccatggct gttattcaag
    42721 atatcatcgc tgcgctctgg caacacgact ttgccgcgct ggcggatcct catattgtta
    42781 gcgttgttta ctttgtcatg tttgccacgc tgtttttaga aaacggcctg ctgcccgcct
    42841 catttttgcc aggcgacagc ttgttgatac tggcaggcgc attgattgcc caaggggtta
    42901 tggattttct gcctacgatt gcgattctga ccgccgcagc aagtctgggc tgctggctaa
    42961 gttatattca ggggcgctgg ttagggaata ccaaaacggt gaaaggctgg ctggcacagc
    43021 ttcctgctaa atatcaccag cgcgccacct gcatgtttga ccgccacggt ctgctggcgc
    43081 tgctggctgg acgttttctt gcatttgtcc gtacgctgct gccaaccatg gcgggaattt
    43141 ccggtctgcc aaaccgccgc ttccagtttt tcaactggtt aagtggattg ctgtgggtca
    43201 gcgtggtaac cagttttggc tatgccttaa gtatgattcc gttcgttaaa cgccatgaag
    43261 atcaggtaat gacgttcctg atgatcctgc caattgcctt gttaaccgct ggcttgttag
    43321 gcacgctgtt tgtggtgatt aaaaaaaaat actgtaacgc ctgacgattt tccccgttcc
    43381 cggctgctgt accgggaacg tatttaattc ccctgcatcg cccgcattct tgccgcatct
    43441 tcccccggcg tcacaccgaa gtaacgttta aactcacggc taaattgcga tgcgctttca
    43501 tagccgacgc gcatcgctgc tgcgctggcc ttcatgccgt catggatgat catcatccgc
    43561 gccttatgca gacggtaatt cttcaaatac tgcaacggcg aggtgctggt gacagactta
    43621 aaattatggt ggaacgccga tacgctcatg ttggcttctg ccgccagttg ctcgacgctc
    43681 aggttttcgg tgtatttatt ctcaattcgt ttcagcacgc ggctaatcag actgaagtga
    43741 gtctggcgac tgaccagcgc cagtaacgcg ccgccgcaag gtccggtcag cacgtagtac
    43801 agaatttcgc ggatgatctg tttgccgaga atacgcgcat ccagtggtcg ctccatcacg
    43861 tcgagtaacc gctccgccgc gaataaaatc tcttctgata acgtggcgga gttaatcccg
    43921 ctggctgcca tcgacggctg gaaatgctca tcttcgccaa tgtccatcaa cagttcctgt
    43981 aactgcaaaa tatcgacatt gagacgcaac cctgccagcg gcacctctga cgtggcatag
    44041 gtttcgcact caaacggcaa cggcaccgtc agcagcaggt attcattggc atcataacga
    44101 aacacgcg
//
LOCUS       DADMGC010000016        43689 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.016, whole genome shotgun sequence.
ACCESSION   DADMGC010000016 DADMGC010000000
VERSION     DADMGC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.016"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            231..1664
                     /locus_tag="JNZ73_000902"
     CDS             231..1664
                     /locus_tag="JNZ73_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAY8731284.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="JNZ73_000903"
     CDS             complement(1672..2730)
                     /locus_tag="JNZ73_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY8731285.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="JNZ73_000904"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="JNZ73_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="JNZ73_000905"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="JNZ73_000905"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HAY8731286.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JNZ73_000906"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JNZ73_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HAY8731287.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JNZ73_000907"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JNZ73_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HAY8731288.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="JNZ73_000908"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="JNZ73_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HAY8731289.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="JNZ73_000909"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="JNZ73_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAY8731290.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIVVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="JNZ73_000910"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="JNZ73_000910"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HAY8731291.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="JNZ73_000911"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="JNZ73_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HAY8731292.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFCCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="JNZ73_000912"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="JNZ73_000912"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HAY8731293.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIHYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="JNZ73_000913"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="JNZ73_000913"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HAY8731294.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="JNZ73_000914"
     CDS             complement(17699..18322)
                     /locus_tag="JNZ73_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HAY8731295.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="JNZ73_000915"
     CDS             18619..18894
                     /locus_tag="JNZ73_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731296.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="JNZ73_000916"
     CDS             complement(18971..19213)
                     /locus_tag="JNZ73_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HAY8731297.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="JNZ73_000917"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="JNZ73_000917"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HAY8731298.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="JNZ73_000918"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="JNZ73_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HAY8731299.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="JNZ73_000919"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="JNZ73_000919"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HAY8731300.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKACVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22592..24289
                     /locus_tag="JNZ73_000920"
     CDS             22592..24289
                     /locus_tag="JNZ73_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY8731301.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24304..24699
                     /locus_tag="JNZ73_000921"
     CDS             24304..24699
                     /locus_tag="JNZ73_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY8731302.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSVRLPRR"
     gene            24657..25037
                     /locus_tag="JNZ73_000922"
     CDS             24657..25037
                     /locus_tag="JNZ73_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HAY8731303.1"
                     /translation="MATTADQRTPAATVTRENDTINLVKDERIIVTPINDIYYAEAHE
                     KMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYILR
                     LKDLDFEVPVSRSKVKEFRQLMHL"
     gene            25050..25907
                     /locus_tag="JNZ73_000923"
     CDS             25050..25907
                     /locus_tag="JNZ73_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8731304.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(25910..28411)
                     /gene="ptsP"
                     /locus_tag="JNZ73_000924"
     CDS             complement(25910..28411)
                     /gene="ptsP"
                     /locus_tag="JNZ73_000924"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY8731305.1"
                     /translation="MLTIQFLCPLPMPNGLHARPARELKEQCSQWQSEITFINHRQNA
                     KADAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAEL
                     TAHPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHS
                     LATLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNM
                     EQVCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTP
                     SQFLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQ
                     CGVLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEA
                     PGAFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGD
                     KSISYLNVPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLD
                     QILWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSND
                     MTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLP
                     LLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPE
                     EDVRPLLALENIFVDQDLSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTG
                     VGFGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVF
                     SQLARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28436..29473)
                     /gene="ypdE"
                     /locus_tag="JNZ73_000925"
     CDS             complement(28436..29473)
                     /gene="ypdE"
                     /locus_tag="JNZ73_000925"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAY8731306.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKILGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29473..30556)
                     /gene="ypdF"
                     /locus_tag="JNZ73_000926"
                     /pseudo
     CDS             complement(29473..30556)
                     /gene="ypdF"
                     /locus_tag="JNZ73_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30571..31818)
                     /locus_tag="JNZ73_000927"
     CDS             complement(30571..31818)
                     /locus_tag="JNZ73_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAY8731307.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31840..32166)
                     /locus_tag="JNZ73_000928"
     CDS             complement(31840..32166)
                     /locus_tag="JNZ73_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HAY8731308.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32385..33350)
                     /gene="glk"
                     /locus_tag="JNZ73_000929"
     CDS             complement(32385..33350)
                     /gene="glk"
                     /locus_tag="JNZ73_000929"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HAY8731309.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33554..34810
                     /locus_tag="JNZ73_000930"
     CDS             33554..34810
                     /locus_tag="JNZ73_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HAY8731310.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAIMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34925..35251
                     /locus_tag="JNZ73_000931"
     CDS             34925..35251
                     /locus_tag="JNZ73_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY8731311.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35392..36630)
                     /gene="mntH"
                     /locus_tag="JNZ73_000932"
     CDS             complement(35392..36630)
                     /gene="mntH"
                     /locus_tag="JNZ73_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HAY8731312.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36966..38168
                     /gene="nupC"
                     /locus_tag="JNZ73_000933"
     CDS             36966..38168
                     /gene="nupC"
                     /locus_tag="JNZ73_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HAY8731313.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38218..40407)
                     /locus_tag="JNZ73_000934"
                     /pseudo
     CDS             complement(38218..40407)
                     /locus_tag="JNZ73_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40615..40690)
                     /locus_tag="JNZ73_000935"
     tRNA            complement(40615..40690)
                     /locus_tag="JNZ73_000935"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40655..40657),aa:Ala,seq:ggc)
     gene            complement(40730..40805)
                     /locus_tag="JNZ73_000936"
     tRNA            complement(40730..40805)
                     /locus_tag="JNZ73_000936"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40770..40772),aa:Ala,seq:ggc)
     gene            41041..41385
                     /locus_tag="JNZ73_000937"
     CDS             41041..41385
                     /locus_tag="JNZ73_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY8731314.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
     gene            41408..41779
                     /locus_tag="JNZ73_000938"
     CDS             41408..41779
                     /locus_tag="JNZ73_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY8731315.1"
                     /translation="MTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGGRARLILV
                     DTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTDIEQEKVA
                     QFLSREGIRNFLARLDIDESA"
     gene            complement(41831..43246)
                     /gene="gltX"
                     /locus_tag="JNZ73_000939"
     CDS             complement(41831..43246)
                     /gene="gltX"
                     /locus_tag="JNZ73_000939"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAY8731316.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43505..43580
                     /locus_tag="JNZ73_000940"
     tRNA            43505..43580
                     /locus_tag="JNZ73_000940"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43538..43540,aa:Val,seq:tac)
BASE COUNT        11592 a        10881 c        10167 g        11049 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaacaatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 gcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccaa
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat gcggaaaatc tcatttgcag aaatagaaag
     4681 tgactgttgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagtggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgtagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggatattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aaacgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaacaaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggtag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccctc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aatgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata ccccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ccgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attatttcat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattgattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgag atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatggttt ttatggcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggtgtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg gcgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgcggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaagc cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccaaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag cgtttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgcccct gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacaga aaatttttct
    22381 acgctaacaa gaaaattacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttc aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgcgt cggtattctc ggcggcatgt tgtgtgagac
    23041 gttgaccatg attctggtaa ttgtctgggc accaactacc gcgttgggga tcgatatcgt
    23101 ctctaaaatc ggcattccaa tgatcctcgg tagcgtctgt atcggcttta ttgtgcttct
    23161 ggtgcaaagc gttgagggcg aaaaagaggc cagcgccgcg cggcaggcca agctggcgct
    23221 ggatatcgcc aacaaaacgc taccgctgtt tcgccatgtc aatagcgagt cattacgcaa
    23281 ggtctgcgaa attatccgcg atgacattca cgccgatgcg gtggcgatta ctaataccga
    23341 tcatgtgctg gcctatgttg gcgtgggtga acataactat cagaatggcg atgacttcat
    23401 tagcccgact acccgtcagg cgatgaatta cggaaaaatc atcattaaaa acaatgatga
    23461 agcccaccgc acaccagaga ttcattccat gctggtgatc ccattgtggg agaaaggggt
    23521 cgtgaccgga acgctgaaaa tttactactg ccacgcgcat cagatcacct cgtcattaca
    23581 ggaaatggcg gtcggtctgt cgcaaatcat ctccacgcaa ctggaggttt cacgcgccga
    23641 gcagctacgt gaaatggcaa ataaggcaga gcttcgcgcc ctgcaaagca aaattaatcc
    23701 ccattttctg tttaacgctc tgaacgctat ttcatcgtca atccgtctga atccggatac
    23761 cgctcgccag ttgatcttta atctgtcgcg ttatctgcgc tataacattg aattaaaaga
    23821 cgatgagcaa atcgatatca aaaaagagct gtatcaaatt aaagactata ttgccattga
    23881 gcaggcccgc tttggcgaca agctgacggt tatctatgat attgatgaag aggtgaattg
    23941 ctgcattccc agcctgctga tccagccgtt ggtggagaac gccattgtcc acggtattca
    24001 gccttgcaaa ggtaaaggcg ttgtcaccat cagcgttgca gagtgcggaa atcgggtacg
    24061 cattgcggtg cgagataccg ggcacggcat cgatccaaag gtgattgagc gggtcgaagc
    24121 gaatgaaatg ccgggcaata aaattggcct gctgaatgtc catcatcgcg tgaagttatt
    24181 gtatggcgag gggctgcata tccgccgcct ggagccgggg acggaaattg cgttttacat
    24241 tcctaaccaa cgcaccccag tcgcctcaca ggctacgtta ttgctttgag ccggagtgat
    24301 attgtgaaag tcatcattgt tgaagacgaa ttcctggcac aacaggaact gagctggcta
    24361 attaaagagc acagccagat ggagattgtc ggcacctttg acgacggtct ggacgtgttg
    24421 aagtttttgc agcataaccg cgtcgacgcc atttttctgg atatcaatat tccgtcgctg
    24481 gatggcgtgt tgctggcgca aaacatcagc cagttcgccc ataaaccgtt tattgtgttc
    24541 atcaccgcgt ggaaagaaca tgcggtagaa gcgtttgaac tggaggcgtt tgactacatt
    24601 ctcaaaccat atcaggagtc acgtattacc gggatgctgc aaaaactgga agcggcctgg
    24661 caacaacagc agaccagcgt acgcctgccg cgacggtaac gcgtgagaat gacaccatta
    24721 atctggtgaa agatgagcga ataatcgtca cgccaattaa cgatatctat tacgccgaag
    24781 cgcacgagaa aatgaccttt gtctatacgc ggcgtgaatc ctacgtaatg ccgatgaaca
    24841 ttaccgaatt ttgcagcaaa ctgccgccgt cgcatttttt ccgctgccat cgctcatttt
    24901 gtgtcaatct gaacaaaata cgcgaaatcg aaccgtggtt taataacacc tacattctgc
    24961 gactgaaaga tctggatttt gaagtgccgg tcagccgcag caaagtgaaa gaatttcgcc
    25021 agttaatgca tctttaatcg ggaactttca tgaaagcacc aggcttgccc gccgatcaac
    25081 aatttttcgc cgatctgttc agcggcctag tgcttaaccc gcaactactg gggcgcgtct
    25141 ggtttgccag ccagcctgcc tcgttaccgg tgggcagttt atgtattgat tttccccgtc
    25201 tggatatcgt gctgcgcggc gaatacggca acctgctgga agcaaagcag cagcgaatgg
    25261 tggaaggaga aatgctgttt attccggcac gcgcggctaa tttaccgatc aacaacaaac
    25321 cggtgatgct gttaagcctg gtgttcgcac cgacctggct tgggttatcg ttttacgata
    25381 gccgcaccac gtcgttgttg catcctgctc gccagatcca gcttcccagc ctgcaacgcg
    25441 gtgaaggtga agcgatgctt accgccctca cccatcttag ccgttcgccg ctggagcaga
    25501 atatcattca gccactggtg ttaagtttgc tgcatctttg ccgcaacgtg gtgaatatgc
    25561 cgccgggtaa ttcgcaaccg cgcggcgatt ttctctatca cagcatttgt aactgggttc
    25621 aggataatta tgcccagccg ctcacccgcg agagcgtggc gcagtttttt aatatcacgc
    25681 ccaatcatct gtcaaaactg tttgctcagc atggaacgat gcgttttatc gagtatgtgc
    25741 gttgggtgcg aatggcgaag gcgaggatga ttttgcagaa atatcatctg tcaattcatg
    25801 aagtggcaca gcgttgcggt tttccggata gcgactattt ttgtcgcgtt ttccggcgtc
    25861 agtttggtct gacgccggga gagtacagcg cccgttttca gggctaacgt cagaaggtta
    25921 attctgtttc cagcagcgtc aggatacttt gtgcatcttg cgcggcaaac aacgactggc
    25981 ggaagttttt attcaccagt ttacgcgcca gctgcgagaa gactttcaca tgattcatcc
    26041 cttcgttagc acccagcgtc agcatgatca ccagttcgac ttcgcccatt tctgactgcc
    26101 agtcaaccgg tttcgccagc cgggcaatgc tgatactgga atgacggatc cactgagatt
    26161 tggtgtgcgg gatcgctacg ccaaaaccaa cgccggtggt aacaatctct tcccgctgcc
    26221 agacatcttc ttccagctca aacggatgtt cagtgcgccc gttaacgccg aggttgccgc
    26281 acaggaactg gatcgcctgc tctttattgc taagatcctg atcaacaaag atattctcca
    26341 gcgccagcag tgggcgaacg tcttcttccg gcgtaaaggc ggtgagtaac gcttcaattt
    26401 cctgcgcact gcggcattca catgcctgac gcgccagttc ccgacacgcc tcgctatcca
    26461 gttgacgaag ctggcttttc accgccggaa tacgcgggct actcatactc agctcatcca
    26521 ggcccagccc aagcagtagc ggcagataac ggctttcacc gcccagttca ccgcaaatgc
    26581 ctacccattt gccccgctga tgcgcagtgg taactatttg ctgcaacatg cgcaggaacg
    26641 atggcgtaat cgggttatat agcggcgata cgcgcgggtt attacgatcg accgcataca
    26701 gatactgggt catatcgttg gagccgatac tgaagaaatc gacctcatcg cagaagtggt
    26761 cgatgatgta gcacaccgac ggcacttcca ccatgatccc aagcgtaatc gtctctgcat
    26821 gacgcaggcc atcgcgctta agctcaacga tcgctttttg gatctcgcct ttcacccata
    26881 agatctgatc gaggctgtga accatcggga tcatcaactg ggcgttgccg aaactggcgg
    26941 cgcgcaaaat ggcccgcagt tgagtgcgga acaggccagc aaattccggg taaatacgta
    27001 ccgcgcgata gccgaggaat gggttctctt cctggggaac attcagataa gaaatgcttt
    27061 tatcgccgcc gatgtccatc gtgcggaaga taatcggctt gtcgcccgcc gccagtagca
    27121 cctgctggta ggcttcaaat tgctcctgct cgtccggcgc gctgtcgcga tccatataga
    27181 gcatttcggt acggaacagc ccgacacctt ccgcgccgtt ggcaaacgcg cctggcgctt
    27241 ccagagcggt gccgatattc gccgcaatat caatacgctt gttatcacgg gaataggcca
    27301 gctgcgcggc agcctgcgcc tgttgttttt ggcgtttatc cgccagcgtc tgcgcgacct
    27361 gataataacc gctcaccgcg tcattcgggt taatcgccag cacgccgcac tgggcgtcaa
    27421 gcactgcagg ttgcccggca taacgggcaa tcgcatccag cggcaagcca ctcagtaccg
    27481 ggatcgccga ggcacgggcc agaatcagtg tatgcgaggt gcgcccggtt ttctccagaa
    27541 tcatacccgc aagatttttc aaatcaaggc tcaaaaactg gcttggggtt aaatcttcag
    27601 ccaccagaat ggtcggtttt tccagcacca gattgttgcg cggcttcagt tccggccagg
    27661 tgatatgcag caactgttcg ctgatatcgc gaatgtcgct gacccgctcg cgcagataat
    27721 cgctggcaga agcagatagt ttggcgcaaa cctgctccat attgctgatg atcgccgccc
    27781 ccagtccctg atgctgttct gtcatcaggc gacggatatt gcctgcaaat tcatcatcct
    27841 gaatcagcga caaatgagcg ctgaggatag ttttgctttc gccgtcacgc tcacgcaatt
    27901 gctgattcag ttgctcggca agcgttgcca ggctgtgctc cagccgggtg gaatcttgcg
    27961 cactggcggg gattgcccga taactgtcga ggctgtcgct ttgtaacagg gtcagcgtac
    28021 ccacgccgac gccgcttgcc agcacattgc cgtacagtaa atccgggtta aggcggctta
    28081 atgaacgcgg cagcggatgc gccgtcagtt ctgcctgcgt cggctgaacg ctgtcgctgt
    28141 cgataaagcg caccaggatg tactcttcca gcacgcgccg cgcctgctct tcatcgctgc
    28201 cgctaatgtt caggctgcaa ctgtcattaa ataaggtgcc agttccaatc aacgccagcg
    28261 agcttttagc atcggccttt gcgttctggc gatggttgat aaaggtgatt tcgctttgcc
    28321 actggctgca ctgttcttta agttcccgcg ccggacgagc atgtagaccg ttaggcatag
    28381 gcaaaggaca gagaaattga atcgttaaca taaccgctcc gggttaatca ggagatcatc
    28441 tgaaatccgt cagttgaaca accgtctcac gcgtaagacg ttgaataagg gcagataaaa
    28501 gttgctccat ctgcaaaatg tcgcggcaat cggcaatcga tgccgcgcaa tgtccgtggc
    28561 gggtggcagg ccccattacc aatgtgggta cgccagtacc ggttaagtgc accgctccac
    28621 cgtccgtgcc gccgttactg aacatatccg cctgcaacgg aacgccaatt tctgccgcca
    28681 cggtttcaat ccacgcggta agttttggcg gcgcaatcag cgatttatcg cttaacacca
    28741 gcatcggccc gttaccaatc tgacgatggt tagccgcgcc ataatcaaag tttttcgccc
    28801 agcaggcggt atcaagcaca atggcgacgt ccggcgacac cgcgcgggta gcagtttgcc
    28861 cgccgcgtaa tcccacctct tcgctggaac ttgccaccag ccacacttcc gcaggcagtt
    28921 cggcgtcgtg cagctcacgt agtaacgtca ccagcagata gcaaccgagg cgatcatcaa
    28981 aggctttgcc catcacccgc tggtgaggga ggacctgaaa agtggtatcg aaggtgacgc
    29041 gatcgcctgg acgaattccc gcctgcatca cttcgtcata ggtgcgcgcg ccaatgtcca
    29101 cgcgcatggc gctaacgtca ttcccctgcc ggtcgccgtc aagcaggcct agaattttgc
    29161 actcttcacg ggtggtgatg cgcaccggct gcagctggcg ggcagccatg cgcacgttgc
    29221 caaccggcag cacgtcaata gcgccttcgc gggagatgct gcgcaccata aatcccactt
    29281 cgtccatatg ggcgcagatc atcaccttcg gacctgtcga ttcattgagg cggatcagca
    29341 ccgatcccag accatcaaat cgcacttctt tttgcaggcg agccgcttct tccagcagga
    29401 tctgccgcac ttcctgttcc gaggaggcga tcgcatctgc ctcgctcaac gcttttaata
    29461 gcgataaatc cattatgcct ctcccgtgag caacactgtt ttcggcatgg cgtagagcac
    29521 ttctgcgcct tgcggggtaa ccagcacaac atcttcgatg cgcacgcccc cttgccctag
    29581 caaataaatc cccggctcca cggtcagcaa catgcctggc tgtagcgtcg tggtgtcccg
    29641 cggtgaaaaa cgcggatctt catggacttc aatgccgata gcgtgaccag tgttatgacc
    29701 gaaatagtcg ccaaatccag actcggtaat gacccggcgc gcggcgtcgt caatctgctg
    29761 gcagcgcacg ccggggcgaa ttgcagagat tgccgcgagc tgtgcctgca ggacaatctg
    29821 atagacgtca aacagcgggt gagattcggc gctcacccct tcgccattca ccagcaaggt
    29881 gcgcgtcata tcagcagtag ccctgataca gcgcaccaaa atcgagagtg acaaactcgc
    29941 ccgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccgcgc cagccgctgg
    30001 cgacaatggt gtcaaaagag gctttttctg cgccctgctg gcgcataaac cactccagtt
    30061 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
    30121 cgcaatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
    30181 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
    30241 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
    30301 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
    30361 agccttgcgc acgggcttcc acctcaacga aatagcgcga atctaccaga atgtgcgcac
    30421 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
    30481 tctgccgtga ggagagaagc accgcatcca gttgttgcac cttaagccag ttgcgcagcg
    30541 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttgccttt
    30601 gcggaacatc atcagacgca ggaacaccac catcagcgca gtaatgattg cccccagcgc
    30661 gatccccgcc atataaacgc ccaggttagt gaccagcggc catgcccaga tagcggattc
    30721 cgggaaccat tgcactgcgc ccagccagac agctgcggtt gagccgacaa tcgcgccgac
    30781 catatatgac ggaatggcgg tgatggggct ttccagcgca aacggaatcg caccctcact
    30841 aatccccata aaggccagga acatcgcggt cttaccctgc ggataaagtt gagcgttgaa
    30901 caggcgtttg ccggttaaac gacggtcaat aatggtcgcc agacccagac caatcggcgg
    30961 aataacgata gcgatagaac gagcggtgac cggtaatacg tggtcagtgg taaagctgaa
    31021 ggcaacgaaa cctgcggctt tgttgatcgg gccaccaagg tcgattgctg tcgccgcggc
    31081 gatccccatt gcgtacatca gcgcgccctt ctcacctgcg gcagtcagca cagtacggat
    31141 accgccgttg atccagccac caaagggggt gatgacgtag tacatcgcca gcatgacaaa
    31201 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatccggtaa
    31261 ctggattttc tggttcatcc atttcacaag gtaaccggca acgatagaaa taatcagcgc
    31321 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
    31381 gttcagcagt tgcgtcggct gggtagacat caacccgccg ataaaaccag ccgggaacgc
    31441 cagtttgcca ccgatagagt tcgccacaaa tgcagcaaac atcggaatgg caaaaccaaa
    31501 caacacgccg ccgaaggact gcgacagcca ggcaaattta agtaaggaga ggtcgaaacc
    31561 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
    31621 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
    31681 catgcgtgaa atccctgaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
    31741 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
    31801 gcgtttttta atggccataa caattatccc tgtcgaatat tactgttgtt cagaggcaat
    31861 catctcttcg atttctttga tgatgcccgc agcgttttta attgcgtcct gcaaagtgat
    31921 ttcataaacg tcgcgtgatt cgaaacgttc gttatcttcc ggggtaactg ccacggaatg
    31981 gatgatgatg gtcgcttcgg cgatatcctg cgccgtcagg cgattctgga tgccgtccgc
    32041 gccctgggtt tcaattttca cttcataacc ggcttctacc gccgcttctt ccagcgcctg
    32101 agcggccata aaagtgtgag ccaggcccat tgggcaggca cataaggcaa tcagtttctt
    32161 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
    32221 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtacggaag
    32281 tgtgacgcag gtcggccttg tgaggggaca caaagaaaaa ttgaattaac tgtatgattt
    32341 aaaatattat cgggagagtt acctcccgat ataacaggaa ggatttacag aatgtgacct
    32401 aaggtctggc gtaaatgtgc accggaaccg agaagacccg gatagtcatg gacgatgaga
    32461 tacaccggaa tatcatggac atattcttta aagcgccctt tatcttcaaa tgcggcacgg
    32521 aaaccggagg ctttgaagaa ctcaaggaag cgcggcacga taccgcccgc aataaacacg
    32581 ccgccaaatg tcccgagatt gagcgccaga ttgccgccaa aacggcccat aatgacgcaa
    32641 aacagcgaca atgcgcggcg gcaatcggtg cagctgtcag ccagcgcgcg ttcggtaata
    32701 tcttttggct tgagattttc tggcaggcgg ttgtcagctt tcacaattgc gcgatacaaa
    32761 ttcaccagcc cagggccaga aagcacgcgc tccgccgaaa catgaccaat ttccgcacgc
    32821 aatatttcga ggataatggc ctcttcttca ctattcggcg caaaatcaac gtgaccgcct
    32881 tcgcccggca agcttaccca acgcttatcg acatggacca gatgcgcaac cccaagcccc
    32941 gttccggcac cgtaaaccgc aataggctta ccttcgaccg gttctgcgcc accaaactga
    33001 atcagatgct cttttttcag catcgggatc gccatcgata cagcggtaaa atcgttaata
    33061 atttccagat ggctaaaacc gagattcttt ttcatttcgg caattgagaa cgcccaggta
    33121 tggttggtca ttgccaccca gtcaccggta attgggcaag cgatggcaat acagccgtct
    33181 ttcacctcga ccttatgttc ttcaagataa acgcgaatga ccgcttcgag gctggggtaa
    33241 tcaagccctg aataggtctt agcctgcgag atttcaccac tggcaatatc acacagagca
    33301 agacgtgcgt tggtgccgcc cacatcaccg actaatgcat actttgtcat tcttcaactg
    33361 ctccgctaaa gtcaaaataa ttctttctca cactgtaaat acttgggggc ataacaacaa
    33421 cgccggaaag gcaggctccc tgtaaatatc gatctgggtc acacaattac tttatcgttt
    33481 cagcaccaat tgcagcgatg cctttttgca agctgggcaa actaagtatc tgaccccgca
    33541 taaggaatag aacatgctcc atccgcgagc cagaaccatg ttgttattat cgctccccgc
    33601 cgtggcaatt gggattgcgt ccagtcttat tctgattgtg gtgatgaaaa tcgcctcggt
    33661 attacagaat ttgctctggc aacgactgcc gggaactctg gggatagccc aggattcccc
    33721 cctctggatc atcggtgtat taacgctaac gggtattgcg gtggggttgg ttatccgttt
    33781 cagccagggt catgccggac cagaccccgc ctgtgaaccg ctgatcggcg caccggttcc
    33841 gccctctgcg ctacctggac ttatcgtagc attaattctc ggtcttgctg gcggcgtcag
    33901 cctggggccg gaacatccga tcatgaccgt caatatcgcc cttgccgtcg ccattggcgc
    33961 tcgtctgtta ccgcgcgtca accgaatgga gtggactatt ttagcctctg ccggaaccat
    34021 cggcgcgctg tttggtacac ctgttgcggc ggcgttgata ttttcgcaaa ccttaaatgg
    34081 cagtagtgaa gttccgctat gggatcgtct ttttgcgccg ttaatggcgg cagcagctgg
    34141 tgcacttact accggattat ttttccatcc ccatttttca ctgcccattg cgcattacgg
    34201 acagatggaa atgaccgata ttctcagcgg tgcaattgtc gcggcgattg ccatcgcagc
    34261 agggatggtc gccgtatggt gcttaccacg gttgcacgcg ataatgaatc aaatgaaaaa
    34321 tccggtgctc gtgctgggta ttggcggatt tattctcgga attctggggg ttattggtgg
    34381 accagtttcg ctgtttaaag ggctggatga gatgcagcag atggtggcaa atcaggcttt
    34441 cagcaccagc gattactttt tgctggcggt aattaaactt gccgccctgg tcgttgctgc
    34501 cgccagtggc tttcgcggtg ggcgaatctt cccggcagtg tttgtcggcg tggcattagg
    34561 gttgatgctg catgagcacg ttcccgccgt tccagcggca ataaccgttt cctgcgctat
    34621 tctcggcatc gtgctggtgg taacacgcga tggctggtta agtcttttta tggcggcagt
    34681 cgttgtaccc aataccacat tgctaccgct gctctgtatc gtcatgcttc cggcatggct
    34741 gttattagca ggtaagccga tgatgatggt caatcgtccg aagcaacagc caccccacga
    34801 taatgtttag caaaaaatgc tctcctttat gatttaagag ttatggcgat gatacggggc
    34861 tttacctccc cgtaatattg cgttaacagg ccgctgacgg cttcgatcgt gaaggagaat
    34921 aacaatgttc aggtcactgt ttctggcggc cgccctgatg gcatttaccc cgcttgcagc
    34981 aaatgcaggt gaaatcaccc tactgccatc gatcaaatta caaattggcg atcgcgatca
    35041 ttacggtaat tactgggacg gtggtcactg gcgagatcgt gattactggc atcgcaatta
    35101 tgaatggcgc aaaaaccgct ggtggcgtca tgataatggc tatcatcgtg gctgggataa
    35161 gcgtaaagca tatgagcgtg gctatcgtga aggctggcgc gatcgtgacg atcatcgcgg
    35221 aaaaggccgc ggacatgggc accgccatta aacgcgtcct ccaatggagc acaatgcctg
    35281 atgcgacgct agcgcgtctt atcaggccta caaaccggct ctttaacgcg tagggcggat
    35341 aaggcgttta cgccgcatcc gccaatagtg ccagatacga cgctcattca actacaatcc
    35401 cagcgccgtc cccaccaaca accagatatt cagcgccacg accagcacta caatcaccca
    35461 gcctgtctgt tttacgcgtt tgctgttcac cagatcgccc atcaacttgc tgtcactggt
    35521 gaaaatcagc agtggaacca gcgccagggc gataccaaaa cttaacagca cctgactcat
    35581 aaccagaatc cgtgtcggat ctaatcccat cagaatgaca ataaatgacg gcaacatggt
    35641 gactgtacga cgcacccaca gcgggatatg gaagcgaatg aatccctgca tcaccacctg
    35701 ccccgccagt gtccccacca ccgttgagga cagtccggca gcaaccagac ttaacccaaa
    35761 gaccgttgcc gcagcatggc ttaacagcgg ttgcagcgtc agataagcct catcaagatc
    35821 ggcaacacca gtatgaccag aaaagtggaa cgccgccgca gctgtagcca tcatcgccag
    35881 attgacaaaa ccggcaatcg tcatggcgat agccacatcc catttggtgg cggaataacg
    35941 ttgttgacgc gaaccgccat gtaaatgctg agtgagcgaa gagtgcaaat aaatcacatg
    36001 cggcataatc gtcgccccta acacgcctgc tgccaggaag accgcctccg aagtaggtaa
    36061 actcgggatc accattcctt tacccagctg cgccaggtta ggctgggaga aaatcaactc
    36121 gacaatgtaa gccgcggcaa caaacaacag taacccgcca atcactttct ccagcggttt
    36181 ttgcccgcga cgttgcagca ttaaaatcag gaaagtcgcg atccccgtca gcaccgcgcc
    36241 ctgcaacaac gaaacaccaa gaatgagttt aaaaccgatc gccgcaccaa taaattccgc
    36301 caggtcggtt gccatcgcaa taatttctgc ctgaacccaa tagaaccaca ctacgggacg
    36361 cggatagtga tcacgaatct gctccgccag atttttaccg gtggcaatcc ctagtttggc
    36421 agagaggatc tgaattagca tcgccatcag gttggcccaa acgacaaccc acagtagctg
    36481 atagccgaag ctagcacccg cctgaatatt ggtcgcaaag ttaccgggat cgatataacc
    36541 aatcgccgca atgaacgcag gtcccattaa tgcgagcctc atcttgcgcg ccgcccgtcc
    36601 gctgctactc tcaacgcgat agttcgtcat cttgtgcctc taaaacatag cctttgctat
    36661 gtttcatgct attctaaacg agaatgatta tcaaattcat ttaaatggat tgtggtgatt
    36721 tctctgatag accaagatta cgattacgaa aagattgatg acttgaatgt tatggttatg
    36781 tttaatgtta gcacatttac ataactttca gcttccatac acaacatagc agaaatgtat
    36841 gacagatcac tatttttgaa gcctgtcaca ggacgtcatt atagtgtgtg tcagatctcg
    36901 ttttccttaa ccatgttaca tagaatgtgc acggaaattt aacctgcctc atatttggag
    36961 caaatatgga ccgcgtcctt cattttgtac tggcacttgc cgttgttgcg attctcgcac
    37021 tgctggtaag cagcgaccgc aaaaaaattc gtatccgtta tgttattcaa ctgcttgtta
    37081 tcgaagtgtt actggcgtgg ttcttcctga actccgacgt tggtttaggc ttcgtgaaag
    37141 gcttctccga aatgttcgaa aaactactcg gatttgccaa cgaagggact aacttcgtct
    37201 ttggtagcat gaatgatcaa ggcctggcat tcttcttcct gaaagtgctg tgcccaatcg
    37261 tctttatctc tgcactgatc ggtattctcc agcacattcg cgtgttgccg gtgattatcc
    37321 gcgcaattgg tttcctgctc tccaaagtca acggtatggg caaactggaa tcctttaacg
    37381 ccgtcagctc cctgattctg ggtcagtcag aaaactttat tgcctataaa gatatcctcg
    37441 gcaaaatctc ccgtaatcgt atgtacacca tggctgccac ggcaatgtcc accgtgtcga
    37501 tgtccatcgt tggtgcatac atgaccatgc tggaaccgaa atacgtcgtt gctgcgctgg
    37561 tactgaacat gttcagcacc tttatcgtgc tgtcgctgat caatccttac cgtgttgatg
    37621 ccagtgaaga aaacatccag atgtccaacc tgcacgaagg tcagagcttc ttcgaaatgc
    37681 tgggtgaata cattctggca ggtttcaaag ttgccattat cgttgccgcg atgctgatcg
    37741 gctttatcgc cctgatcgcc gcgctgaacg cactgtttgc caccgttact ggctggtttg
    37801 gctacagcat ctccttccag ggcatcctgg gctacatctt ctatccgatt gcatgggtga
    37861 tgggtgttcc ttccagtgaa gcactgcaag tgggcagtat catggcgacc aaactggttt
    37921 ccaacgagtt cgttgcgatg atggatctgc agaaaattgc ttccacgctc tctccgcgtg
    37981 ctgaaggcat catctctgtg ttcctggttt ccttcgctaa cttctcttca atcgggatta
    38041 tcgcaggtgc agttaaaggc ctgaatgaag agcaaggtaa cgtggtttct cgcttcggtc
    38101 tgaaactggt ttacggctct accctggtga gtgtgctgtc tgcgtcaatc gcagcactgg
    38161 tgctgtaaaa ccatacataa aaaagccggg gatcattccc cggctttttt acgcctgtta
    38221 atcagctaat ggctgcgggc gaccaatcaa atacccttgc agatattgca ccccgagctt
    38281 atgcaatagc gcctgctgct gttgcgtctc gacaaactcc gcgaccacac tcaatgactt
    38341 cgctttcgcc agatcggtaa ttgatctcac aatcatcgca tccagcgtat tcgtgacaat
    38401 atctttcaca aagacgccat caattttgat gatatcagcc tgcaaacgct ttaaccgttc
    38461 gtagttggca tatccggtgc caaagtcatc aatcgcaatc cggaaaccaa acttatgcag
    38521 ctgctcgatg ttgtacatgc tgctttctgc gttagaaaac gcctgctcct cggtgatctc
    38581 aagaatgacc gcctgcgggg agatgtgata acgcttaaac agacgaataa tccgtccggc
    38641 aatattcttt tgcagcagcg tgagcggcat taaattgact gaaaagcgcg gtcctttttt
    38701 gtcgcaaggg tgtgttgcca gccacttcaa caaggattcc agcacttgca aatcaaaacg
    38761 cgcgctaagg ttaaactgag caataagggg cagaaactta tccggggtca taatgccgcc
    38821 gtcatatttc agtcgcgcga ggatctcatc ataaccttca ccctctttgt tgcgaattgg
    38881 ctgggcgtag agcagcaaat caccctgatc taacgccgtg cgaatggtat tcagcaatag
    38941 cacctgttta gtggtctgcc cggaaaccat ctcctctctg ctatccagcg ccagcacatg
    39001 atgatgtgcg caggattgct ccgccagcca gcttaactgc cccaacaagg gttgcagggt
    39061 ttcctgattt ccatcaaaac gcccccaggc agcaccatag cccatatcca gcccggtatt
    39121 gttccagtga atttgccgac tattcaggat gttaaccata tgctggagtc gcccttccgt
    39181 ttccggcccg ctcagcacta acagcagttc actacccggc aattgataca acttttcgtt
    39241 ttcctgcatc aacggcagca gcgtacggca aattgagcgg atacagtgaa cgcgcatcat
    39301 taagccgtaa tgacgactca taaactcaag attatcaatg cgcaggcagc aaaaactctt
    39361 gcccgcctct tgctccggcg cttgttccaa cgcacgaaag ttgggtagga gcgttaacgg
    39421 atctgttagc gcctgcaaat gccagcggcg attaagccat tcactgcgat gataaatgcg
    39481 caccatatag agcaggcaaa cgctaaagga aatcagcacc gcgagaataa atgccagcga
    39541 atattcggtt tccacccctt gcaaaaagtt ctggttgtaa ttcagaaggc aaagcgttga
    39601 aacagcctag gtgagattta aaaacggata gcgaagatta ccgaccccga gggtaaagat
    39661 gatgaagaaa acgggtacca ggtaaccggc aataaagtcg ttttcataag gtgtgcacaa
    39721 cagaagtagc agcacgctta acgctgccag ccagcttaag gtaaatgcgc gtttctcttt
    39781 gcccaacgac ggagcgatat ccctgcgcca caatatctgc gcaaagtggg gacttacaat
    39841 catgcgagtg agatagtaga aaagcatgtt gtaaatcagc actgcggtga aaaggcttag
    39901 taaatcaacg accgtgaaaa tggcatccgc atcgccgaaa aaggtggata tctttagcgg
    39961 gaaatcaaag aagctcccca caagatacat gctgcatttg atgccgatcg gcgtcaccaa
    40021 accaagccag aacagacgtt gccagacata tcggctggtc aatccataac gccaacgcgt
    40081 ccccagctgc cagcgtaata ttgcgcaggc acacaacacg acaaacgtct ggctgccgag
    40141 catgaccgca gtctgtaaaa aagagagatg aaagttccac agattggtgc aaaacatccc
    40201 gcataaaacg ggaacgacac cgcgccagcc aaagataaac aagattgaca gcattacgca
    40261 cagtggcatc cacgccagaa agatataact ggaatggata atggcaagtg gcgaaataaa
    40321 acgggatagc tgaataagta ccacggtgag cgtaaacgct agtgtgaata tcttgatatt
    40381 ttttatcagg ttatgctcca caaacatgag tacactactc tcactgatcg gggttttcag
    40441 gcacacaaat aataagttta ttcgcatcgc gaagcaaaca ggggccagcc tgttgccgat
    40501 ttttcatcca atgatgcttt caccaggcgg cgctaacccg gacgtaaact gtattacaaa
    40561 agcggcaaaa agcagagaca aaaaaccccc gctttgcagc gagggttgga aatttggtgg
    40621 agctaagcgg gatcgaaccg ctgacctctt gcatgccatg caagcgctct cccagctgag
    40681 ctatagcccc acgatgcgtt tacgtaccaa atttgctggg tgcaaaattt ggtggagcta
    40741 agcgggatcg aaccgctgac ctcttgcatg ccatgcaagc gctctcccag ctgagctata
    40801 gccccgtcac gtaaagcttg tcgagttgac gggcggcatc atatgaattc cgcccgaatg
    40861 tgtcaacggc aaattgcaac gtgtagtttc aatcgctgaa aaatcaggca aatgaacaat
    40921 tttggaaaac cgctcgcatt cagtagttat tcatgtcacg gtttcctgta aagtggtgtt
    40981 ataaaatgaa ctactaatag acccacatac attcagggaa ttgttatgtc caaggagagg
    41041 atgacaccag atgaacttgc cagactgacc ggctatagcc gccagaccat taataaatgg
    41101 gtacgcaagg aaggctggac aacgtcacca aaacctggcg tccagggtgg taaagcccga
    41161 ctggttcacg tcaatgaaca ggttcgtgaa tatattcgca atgctgaacg tccagaaggt
    41221 cagggagaag cgcctgccct ttctggtgat gcgccgcttg aagtattgct ggtgacactg
    41281 gcgaaagaga tgacgccagt tgaacaaaaa cagtttacat ccttgcttct gcgggaaggg
    41341 attatcggat tgttacaacg cttagggatt cgcgatagca aataatatga aaagattacg
    41401 caataaaatg accaccgaag aactggctga atgcctcggt gtggctaaac aaactgttaa
    41461 ccgttggatc agagaaaaag gctggaaaac ggaaaaattt cccggcgtga aaggcggtcg
    41521 tgccagactt attctggtcg atacgcaagt ttgcgagttt attcagaaca cgccagcctt
    41581 ccataacaca ccaatgttga tggaagctga agagagaatt gctgaatacg ctcctggagc
    41641 acgcgccccg gcgtatcgac aaatcatcaa cgcgatagat aatatgacag acattgagca
    41701 ggaaaaagtc gcgcaatttt tgtcacgtga aggaattcgc aatttcctcg cccgtctcga
    41761 tattgacgaa tcagcataaa taaaaaacgg caggatatta tctcctgccg tttatctttt
    41821 tacacgctaa ttactgctga ttttcgcgtt cagcaataaa atccagcgct ttgttgatac
    41881 gctcgatact gcgggtctta ccaattgcgt gaacggtaac atccagtgct ggagactgcc
    41941 ccgcaccggt tacggcgaca cgcagcggca taccaacttt acccataccc acttccagct
    42001 catccgccgt cgcctgaata gcgtgatgaa cgttttcagc ggtccagtca gtaatcgcgg
    42061 ccagtttgtc acgaaccact tccagcggct gacgcgctac cggacgcaga tgttttttcg
    42121 ccgcatcggc atcgaactca gcaaaatctt cgtagaaata acggcagctc tgtgccatct
    42181 ctttcagcgt cttgcagcgt tcgcccagca gtttcaccag atcagccagc tgcgggccgt
    42241 tacgggtatc gatattttcc tgctcaatgt gccactgtaa gtgagtagca acatactccg
    42301 gcggcagcgc gttaatgtag tgatggttca gccacagcag cttgtcggtg ttgaacgcac
    42361 tggcagattt gctgacggca ttcaaagtga agtatttgat catctcttca cgagtgaaga
    42421 tttcctgatc gccgtgggac cagcccagac gcaccagata gttcagcagt gcttctggca
    42481 aataaccgtc atcacgatac tgcattacgc tgactgcccc gtgacgtttg gacagttttt
    42541 taccgtcatc gccattgatc atagaaacgt gcgcgtaaac cggcaccggc gctttcaggg
    42601 ccttaagaat gttgatctgg cgcggcgtgt tgttgatatg gtcttcgcca cggataacgt
    42661 gggtgatttc catatcccag tcatcgacaa ccacacagaa gttataggtt ggggaaccat
    42721 cggtacggcg gataataaga tcgtccagtt cctggttgct gaactcgatc ggaccacgga
    42781 tctgatcgtc aaaaacaaca gaaccttcct gcgggttagc aaaacgtaca acacacggtt
    42841 catcatcagc atgatgctca tggctgtggc ggcagcgacc gtcataacgc ggcttctcac
    42901 ctttcgccat ttgctcttcg cgcagcgctt ccaggcgctc tttagagcaa tagcatttat
    42961 aagcagtgcc ctcttccagc atctgatcga tcaccgcgtt gtagcgatca aaacgtttgg
    43021 tctggtagta cggaccttca tcccactcca ggctcagcca gttcatgcca tccataatgg
    43081 cttcgatagc ttccggcgtg gaacgctcaa gatcggtgtc ttcaatacgc agcacgaact
    43141 caccgccgtg gttacgtgca aaaagccagg agtaaagagc agtacgcgcg ccgccaacgt
    43201 gcagatagcc tgttgggctt ggcgcgaagc gagttttgat tttcatgaaa tggccttacg
    43261 tttagaaaga tgccgacaac cggcaaatcc tggaaaaatt gagtgggcga tattctatca
    43321 ctcccgcctg attcctcaat gtagttcggg tttctacctt acgcattatt gttttcgttg
    43381 acaaattgcg caacacggtc tgttttgcga tcatcttgct taaatttacg acgaacgaac
    43441 aatttcttta gaaaatgcgt tgactcattt tgaactctcc ctataatgcg actccacaca
    43501 gcgggggtga ttagctcagc tgggagagca cctcccttac aaggaggggg tcggcggttc
    43561 gatcccgtca tcacccacca actactttat gtagtctccg ccgtgtagca agaaattgag
    43621 aagtgggtga ttagctcagc tgggagagca cctcccttac aaggaggggg tcggcggttc
    43681 gatcccgtc
//
LOCUS       DADMGC010000017        43444 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.017, whole genome shotgun sequence.
ACCESSION   DADMGC010000017 DADMGC010000000
VERSION     DADMGC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.017"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..413
                     /locus_tag="JNZ73_000941"
     CDS             <1..413
                     /locus_tag="JNZ73_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8731317.1"
                     /translation="MYIGAPLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKL
                     FWRPKGLAYPLGEAPAKLRCTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFD
                     KYQFSLPKGAKNSSVTYRTINDYGAETPQLNCKS"
     gene            438..3037
                     /locus_tag="JNZ73_000942"
                     /pseudo
     CDS             438..3037
                     /locus_tag="JNZ73_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3028..4098
                     /locus_tag="JNZ73_000943"
     CDS             3028..4098
                     /locus_tag="JNZ73_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8731318.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESSDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQVIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4110..4652
                     /locus_tag="JNZ73_000944"
     CDS             4110..4652
                     /locus_tag="JNZ73_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8731319.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGAGVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIQLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4660..5175
                     /locus_tag="JNZ73_000945"
     CDS             4660..5175
                     /locus_tag="JNZ73_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8731320.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTLDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5141..5878
                     /locus_tag="JNZ73_000946"
     CDS             5141..5878
                     /locus_tag="JNZ73_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAY8731321.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRHEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5989..6999
                     /gene="pyrD"
                     /locus_tag="JNZ73_000947"
     CDS             5989..6999
                     /gene="pyrD"
                     /locus_tag="JNZ73_000947"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HAY8731322.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7173..7715
                     /gene="zapC"
                     /locus_tag="JNZ73_000948"
     CDS             7173..7715
                     /gene="zapC"
                     /locus_tag="JNZ73_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HAY8731323.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7712..8821)
                     /gene="ycbX"
                     /locus_tag="JNZ73_000949"
     CDS             complement(7712..8821)
                     /gene="ycbX"
                     /locus_tag="JNZ73_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HAY8731324.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            9065..11173
                     /gene="rlmKL"
                     /locus_tag="JNZ73_000950"
     CDS             9065..11173
                     /gene="rlmKL"
                     /locus_tag="JNZ73_000950"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HAY8731325.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11185..13092
                     /locus_tag="JNZ73_000951"
                     /pseudo
     CDS             11185..13092
                     /locus_tag="JNZ73_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            13222..14475
                     /gene="pqiA"
                     /locus_tag="JNZ73_000952"
     CDS             13222..14475
                     /gene="pqiA"
                     /locus_tag="JNZ73_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HAY8731326.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAVVIFLASIMVPTLKMSAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14480..16120
                     /gene="pqiB"
                     /locus_tag="JNZ73_000953"
     CDS             14480..16120
                     /gene="pqiB"
                     /locus_tag="JNZ73_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HAY8731327.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16117..16680
                     /gene="pqiC"
                     /locus_tag="JNZ73_000954"
     CDS             16117..16680
                     /gene="pqiC"
                     /locus_tag="JNZ73_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HAY8731328.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16936..17103
                     /gene="rmf"
                     /locus_tag="JNZ73_000955"
     CDS             16936..17103
                     /gene="rmf"
                     /locus_tag="JNZ73_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HAY8731329.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17173..17691)
                     /gene="fabA"
                     /locus_tag="JNZ73_000956"
     CDS             complement(17173..17691)
                     /gene="fabA"
                     /locus_tag="JNZ73_000956"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HAY8731330.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMMDRVVKIT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17760..19520)
                     /locus_tag="JNZ73_000957"
     CDS             complement(17760..19520)
                     /locus_tag="JNZ73_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HAY8731331.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPHWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19705..20157
                     /gene="matP"
                     /locus_tag="JNZ73_000958"
     CDS             19705..20157
                     /gene="matP"
                     /locus_tag="JNZ73_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HAY8731332.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20233..21279)
                     /gene="ompA"
                     /locus_tag="JNZ73_000959"
     CDS             complement(20233..21279)
                     /gene="ompA"
                     /locus_tag="JNZ73_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HAY8731333.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21635..22144)
                     /gene="sulA"
                     /locus_tag="JNZ73_000960"
     CDS             complement(21635..22144)
                     /gene="sulA"
                     /locus_tag="JNZ73_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HAY8731334.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22363..22992
                     /gene="sxy"
                     /locus_tag="JNZ73_000961"
     CDS             22363..22992
                     /gene="sxy"
                     /locus_tag="JNZ73_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HAY8731335.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(22955..25117)
                     /gene="yccS"
                     /locus_tag="JNZ73_000962"
     CDS             complement(22955..25117)
                     /gene="yccS"
                     /locus_tag="JNZ73_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HAY8731336.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQAPQETPVSA"
     gene            complement(25127..25573)
                     /locus_tag="JNZ73_000963"
     CDS             complement(25127..25573)
                     /locus_tag="JNZ73_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAY8731337.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25696..27750
                     /gene="helD"
                     /locus_tag="JNZ73_000964"
     CDS             25696..27750
                     /gene="helD"
                     /locus_tag="JNZ73_000964"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HAY8731338.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSDVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(27782..28240)
                     /gene="mgsA"
                     /locus_tag="JNZ73_000965"
     CDS             complement(27782..28240)
                     /gene="mgsA"
                     /locus_tag="JNZ73_000965"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAY8731339.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28336..28998)
                     /locus_tag="JNZ73_000966"
     CDS             complement(28336..28998)
                     /locus_tag="JNZ73_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HAY8731340.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29171..29584
                     /locus_tag="JNZ73_000967"
     CDS             29171..29584
                     /locus_tag="JNZ73_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAY8731341.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29629..29946)
                     /gene="hspQ"
                     /locus_tag="JNZ73_000968"
     CDS             complement(29629..29946)
                     /gene="hspQ"
                     /locus_tag="JNZ73_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HAY8731342.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30004..31194)
                     /gene="rlmI"
                     /locus_tag="JNZ73_000969"
     CDS             complement(30004..31194)
                     /gene="rlmI"
                     /locus_tag="JNZ73_000969"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HAY8731343.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31289..31567
                     /gene="yccX"
                     /locus_tag="JNZ73_000970"
     CDS             31289..31567
                     /gene="yccX"
                     /locus_tag="JNZ73_000970"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAY8731344.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31564..31893)
                     /gene="tusE"
                     /locus_tag="JNZ73_000971"
     CDS             complement(31564..31893)
                     /gene="tusE"
                     /locus_tag="JNZ73_000971"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HAY8731345.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(31984..32643)
                     /gene="yccA"
                     /locus_tag="JNZ73_000972"
     CDS             complement(31984..32643)
                     /gene="yccA"
                     /locus_tag="JNZ73_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HAY8731346.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(32850..32937)
                     /locus_tag="JNZ73_000973"
     tRNA            complement(32850..32937)
                     /locus_tag="JNZ73_000973"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32901..32903),aa:Ser,seq:tga)
     gene            33363..34481
                     /gene="hyaA"
                     /locus_tag="JNZ73_000974"
     CDS             33363..34481
                     /gene="hyaA"
                     /locus_tag="JNZ73_000974"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HAY8731347.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLTKDVILSLISLYYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPSCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34478..36236
                     /gene="hyaB"
                     /locus_tag="JNZ73_000975"
                     /pseudo
     CDS             34478..36236
                     /gene="hyaB"
                     /locus_tag="JNZ73_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
     gene            36255..36962
                     /gene="hyaC"
                     /locus_tag="JNZ73_000976"
     CDS             36255..36962
                     /gene="hyaC"
                     /locus_tag="JNZ73_000976"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HAY8731348.1"
                     /translation="MQQKSDNIVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            36959..37546
                     /locus_tag="JNZ73_000977"
     CDS             36959..37546
                     /locus_tag="JNZ73_000977"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HAY8731349.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGRLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            37543..37941
                     /gene="hyaE"
                     /locus_tag="JNZ73_000978"
     CDS             37543..37941
                     /gene="hyaE"
                     /locus_tag="JNZ73_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HAY8731350.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            37938..38795
                     /locus_tag="JNZ73_000979"
     CDS             37938..38795
                     /locus_tag="JNZ73_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HAY8731351.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINSTGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            38929..40473
                     /gene="appC"
                     /locus_tag="JNZ73_000980"
     CDS             38929..40473
                     /gene="appC"
                     /locus_tag="JNZ73_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HAY8731352.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            40485..41621
                     /gene="appB"
                     /locus_tag="JNZ73_000981"
     CDS             40485..41621
                     /gene="appB"
                     /locus_tag="JNZ73_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY8731353.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41633..41725
                     /gene="cbdX"
                     /locus_tag="JNZ73_000982"
     CDS             41633..41725
                     /gene="cbdX"
                     /locus_tag="JNZ73_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HAY8731354.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDLRLKS"
     gene            41805..43103
                     /locus_tag="JNZ73_000983"
     CDS             41805..43103
                     /locus_tag="JNZ73_000983"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HAY8731355.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(43218..>43444)
                     /locus_tag="JNZ73_000984"
     CDS             complement(43218..>43444)
                     /locus_tag="JNZ73_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731356.1"
                     /translation="AVSDAAVVGRSVGTSLLVARFGLNTAKEVSLSMQRLEQAGVNIK
                     GAILNGVIKRASTAYSYGYNYYGYSYSEKE"
BASE COUNT        10809 a        10583 c        11561 g        10491 t
ORIGIN      
        1 ttatgtacat tggtgcgccg ttggcgaaag acagagaaac ccttttcttc actagcgtac
       61 gggcagtccc ttcaacaacg aagcgggaag agggaaatac cctgaagatt gccacacaaa
      121 gcgtcatcaa acttttctgg cgaccaaaag gtttagcgta tcccttaggc gaggctccgg
      181 cgaaactgcg ttgcacttcg tcagctgaca tggttacggt cagtaaccca acaccttatt
      241 tcattaccct gacagacctg aaaataggtg gaaaagtagt taaaaatcaa atgatttccc
      301 cctttgataa ataccaattc tctctgccaa agggggccaa aaatagcagc gtaacgtatc
      361 gaaccatcaa tgactacggg gcggaaacgc cgcaactcaa ctgtaaatcg taagccgtct
      421 tcagttaaga gagcgagatg tatagaactc atcgacaaca cagcctgtta agctctggtg
      481 gagtgccatc gtttattggt gggctggtgg tgtttgtgtc ggcagcgttc aatgcacaag
      541 ctgaaacctg gttcgatcct gcttttttca aagatgatcc ctcaatggtg gccgatttgt
      601 ctcgtttcga aaaaggacaa aaaataacgc caggggttta tcgtgtcgat attgttctga
      661 atcagacaat tgtagatacg cgcaacgtca attttgttga gataacgcca gagaagggga
      721 ttgccgcctg tttgacgact gaaagcctgg atgcaatggg tgttaatact gatgcgtttc
      781 cggcttttaa acaactggac aaacaagcgt gtgtgccatt ggcggagatt attccggatg
      841 ccagcgtaac gtttaatgtg aataaactcc gtctggaaat ttcagtaccg caaatcgcca
      901 tcaaaagtaa tgcccgtggt tatgtccccc ctgaacgttg ggatgaaggg atcaacgcgc
      961 tattactggg atattcgttt agcggggcta acagtattca tagcagcgca gacagtgatt
     1021 ctggcgacag ctattttctg aatttaaaca gtggcgttaa tttaggccca tggagattgc
     1081 gcaacaattc aacatggagt cgcagtagtg gccaaaccgc agaatggaag aatctcagca
     1141 gctatttgca gcgggcggtt attccactaa aaggcgaact gaccgtaggt gatgattata
     1201 ctgcaggcga ttttttcgat agtgtcagct ttcgtggtgt gcagctggcg tcagatgaca
     1261 acatgctgcc agacagcctg aaagggtttg cgcctgtggt gcgtggtatc gccaaaagca
     1321 atgcccagat aacgattaag caaaatggtt acaccattta ccaaacttat gtatcgcctg
     1381 gtgcttttga aattagtgat ctctattcca cgtcgtcgag cggtgatttg ttagttgaaa
     1441 tcaaagaagc ggacggcagc gtcaatagct acagcgtacc gttttccagc gtgccattac
     1501 tccagcgtca ggggcgaatc aaatacgcgg tgacactggc gaaatacaga accaatagta
     1561 atgaacagca ggagagcaaa tttgcccagg ccacgttgca gtggggcgga ccgtggggaa
     1621 cgacatggta tggtggtgga caatatgctg aatattaccg tgccgccatg tttggtctgg
     1681 gatttaacct tggtgatttc ggagcaattt cgttcgatgc gacccaggcg aagagtacgc
     1741 tggcagagca aagcgaacat aaaggtcagt catatcgttt tctgtatgcc aaaacgctca
     1801 accaattggg cactaatttt caattgatgg gctatcgcta ttcgacgtcg ggtttctaca
     1861 ccctttccga caccatgtat aaacatatgg atggctacga atttaatgac ggtgatgatg
     1921 aagatacgcc gatgtggtcg cgttattaca atttgtttta caccaaacgt ggcaaactgc
     1981 aggtcaatat ctcccagcaa ttaggcgagt acggttcgtt ttatttaagt ggtagccagc
     2041 aaacttactg gcataccgat caacaggatc ggctattaca gtttggctac aacacgcaaa
     2101 ttaaagatct ctcgctgggg gtttcctgga actacagtaa gtcccgtggt caacctgatg
     2161 ctgatcaggt gtttgcacta aatttttccc tgccgctcaa tctgttgctc cccagaagta
     2221 atgatagcta taccaggaaa aaaattacgc ctggatgacc tctaacacca gtatcgataa
     2281 cgaagggcac attacacaaa acctgggttt aacggagaca ctactcgatg acggtaacct
     2341 gagctacagc gtgcaacagg gatataacag cgaggggaaa acggctaatg gtagcgccag
     2401 catggactac aaaggggcgt ttgcagatgc ccgagtgggc tacaactaca gcgataacgg
     2461 cagtcaacaa caactgaact acgctctttc aggcagttta gttgcccatt cacagggcat
     2521 taccctgggg caatcgctgg gggaaactaa cgtgctgatt gtagcaccag gcgcagagaa
     2581 tactcgtgtg gcgaacagca ccgggctgaa aactgactgg cgcggatata ccgttgtccc
     2641 ctatgccact tcttatcggg aaaatcgaat cgcacttgat gcggcatcgt taaaacgtaa
     2701 cgtggatctt gaaaatgcag tagtcaacgt ggttcccacc aaaggggcgt tggttctggc
     2761 ggagttcaat gcccatgcgg gagcaagggt attaatgaaa acatcaaagc agggtatacc
     2821 gctgcgtttt ggcgcgatag cgacgctgga cggcatacag actaatagcg gcattattga
     2881 tgatgatggc tcgctctata tgtccggttt gcccgctcag ggagcgataa ctgtacgctg
     2941 gggcgaagct cccgatcaaa tttgtcatat cagttaccag cttaccgaac aacaaattaa
     3001 ctctgcgatt acgcgaatgg atgccatatg cagataatct ttggagaaaa atgcgtgtta
     3061 ttactacgac tattttttgc cgccgtctta atgctatggt gcgctcaaac tgctgcttat
     3121 atcgggcagt gtcataccac tcaggggaat ccgtatattg gcgtcaattt tggcgttaaa
     3181 actctggatg aagaagaaaa tacggctgga gtagttaaag acaaatttta tcagtggaac
     3241 gaatcgagtg attattatgt ttcctgtgat tgcgataaag acaatgtcag aagtggccga
     3301 tgggcattcg ccgcggattc accgttagtc tatttaggcg acaactggta caaaattaat
     3361 gactatcttg ccgccaaagt tttattgcag gttaaaggca gttctcctac tgcggttcct
     3421 ttcgaaaacg tgggcacagg ggcagataca cgatggcata tttgcgatcc cggcggtcaa
     3481 cgtttaggtg gccagggggc tagcggtaat agcggtagct tttccctgaa aatattgcag
     3541 ccgttcgttg gttcggtcgt cattcctcct atggcgctgg cgcgattatt tgaatgctac
     3601 aacatacccg caggtgattc ctgcacgact actggtacac cggttttagt gtattacctg
     3661 tctggtacga tcaattcact tggctcatgt tccgtcaatg ccggagaaac aatcgaggtt
     3721 gatctgggcg acgtatttgc ggctaacttt cgtgttgtag ggcataagcc tcttggggcc
     3781 agaacggcag aacttgcaat tccggtcagg tgtaacacgg gaaacgcagg gttagttaac
     3841 gtcaatctga gtctgacggc aacctcagac cccagctatc cccaggtgat taagacgtca
     3901 cgtcctggcg tgggcgtggt ggtgaccgat agccagaaca acattatttc ccctgctggt
     3961 gggacactac cgctctctat tcctgatgat gcagacagta tcgcgcgaat gaatgtctat
     4021 ccagtcagca cgacaggcgt accaccagaa accgggcgat ttgaagccac ggcaacggtg
     4081 agaataaatt ttgattaacg cgtgaacgta tgaagaaaaa aacgatatat cagtgcgtta
     4141 ttttgttctt tagccttctt aacatccatg tcgggatggc tgggcctgaa caagtcagta
     4201 tgcatattta tgggaatgtg gtcgatcagg gctgtgatgt cgccaccaaa agtgcattac
     4261 aaaatattca tattggtgat tttaatatca gtgattttca ggccgcgaat accgtaagca
     4321 ctgctgctga tttgaatatt gatatcaccg gttgtgccgc tggtattact ggcgcgggcg
     4381 tcctttttag cggcgaggct gacacccttg cgccgacact gctcaaacta actgacacag
     4441 gcggaagcgg tggtatggca acggggattg ccgtgcaaat tcttgatgcg caaagtcagc
     4501 aagaaatcca gctcaatcag gtccagcctc ttacgccctt aaaagccggg gataacacac
     4561 tcaaatatca acttcgttat aagtccacaa aggcgggagc aacgggcggt aatgcgacgg
     4621 cggttctcta ttttgatctg gtttaccagt gaaggagtga tgttgaaacg gataatctgg
     4681 attctgttct tactgggatt aacggggggc tgtgagctat ttgcccatga tggcacggtc
     4741 aacattagcg gatcgtttcg ccgtaataca tgcgtgctgg cacaggatag caagcaaatc
     4801 aatgtgcagt taggcgatgt ctcgctgaca cgtttttttc atgggaatta tggcccggag
     4861 aaatctttca tcattaattt gcaggattgc ggaacagatg tcagcacggt tgatgtcacc
     4921 ttttcaggaa ccctcgacgg agtgcagagc gaaatgctgt ccattgaaag tggtacggat
     4981 gcagccagtg ggctggcaat tgcgattctg gatgatgcga agatattgat tccgcttaac
     5041 caggcgagta aagactacag cctacatagc ggtaaggtgc cattaacttt ttatgcgcaa
     5101 ttgcgacctg tcaatagcga tgtgcagtcg ggtaaagtga atgccagcgc gacatttgta
     5161 cttcattatg actaatacgt ggaacagatt agcgctcttg attttcgccg ttttatcgct
     5221 gctggtggcg ggtgaattgc aggcaggagt ggtggtcggc ggaacgcgat ttatctttcc
     5281 ggcagacaga gaatcgatat ctattttact gactaatacc agtcaggaat cttggcttat
     5341 taacagcaaa atcaatcgcc caacgcgttg ggccgggggg gaagcgtcga cagtgccagc
     5401 actattactg gccgctccgc cacttattct cctgaagccc ggtactacag gcacgttgcg
     5461 cttgctgaga acggaaagcg acatcttgcc tgtggatcgc gaaacgctat ttgagttaag
     5521 cattgccagc gtgccatccg gcaaagttga aaatcagagc gtaaaagtgg cgatgcgctc
     5581 ggtatttaaa ctgttctggc gacacgaagg tttgccgggc gacccgctgg aagcttatca
     5641 acaattacgc tggacacgga attcgcaggg tgtacaactc actaacccaa cgccttacta
     5701 cattaacctg attcaggtga gcgtaaatgg taaagcgtta agtaatgcgg gagtcgtgcc
     5761 gcctaaaagc cagcgtcaga caaactggtg tcaggtaata gcaccgtgtc atgtcgcctg
     5821 gcggacgata aatgattacg gcgggttgag tgcaaagaag gagcaaaatc tgccctgaaa
     5881 caggttctga aaacgtttgc gttttttttg ccgcaggtca attccctttt ggtccgaact
     5941 cgcacataat acgcccccgg tttgcacacc gggaatccag gagagttcat gtactacccc
     6001 ttcgttcgta aagccctttt ccagctcgat ccagagcgcg ctcatgagtt tacttttcag
     6061 caattacgcc gcattaccgg aacaccgttt gaagcactgg tgcggcagaa agtgcctgcg
     6121 aaacctgtta actgcatggg cctgacgttt aaaaatccgc ttggtctggc agccggtctt
     6181 gataaagacg gggagtgcat tgatgcgtta ggcgcgatgg gatttggatc gatcgagatc
     6241 ggtaccgtca cgccacgtcc acagcctggt aatgacaagc cgcgtctctt tcgtctggta
     6301 gatgccgaag gtttgatcaa ccgtatgggc tttaataatc ttggcgttga taacctcgta
     6361 gagaacgtaa aaaaggccca ttatgacggc gtcttgggta ttaatatcgg caaaaataaa
     6421 gatacgccag tggagcaggg caaagatgac tatctgattt gtatggaaaa aatctatgcc
     6481 tatgcgggat atatcgccat caatatttca tcgccgaata ccccaggatt acgcacgctg
     6541 caatatggtg aagcgctgga tgatctctta accgcgatta aaaataagca aaatgatttg
     6601 caagcgatgc accataaata tgtgccgatc gcagtgaaga tcgcgccgga tctttctgaa
     6661 gacgaattga tccaggttgc cgatagttta gttcgccata atattgatgg cgttattgca
     6721 accaatacca ctctcgatcg ttctctggtt cagggaatga aaaattgcga tcaaaccggt
     6781 ggcttaagtg gtcgtccgct tcagttaaaa agcacagaaa ttattcgccg cttgtcacta
     6841 gaattaaacg gtcgcttacc gatcatcggt gttggcggca tcgactcggt tatcgctgcg
     6901 cgtgaaaaga ttgctgcggg tgcctcactg gtgcaaattt attctggttt tatttttaaa
     6961 ggtccgccgc tgattaaaga aatcgttacc catatctaat tatttcttcg aattcctctt
     7021 tataagataa tcagggcttt attttcagcc ctggttgttt tatattcatc gctgttgctt
     7081 atttagacat tttgtacttt tattgttgag gttattaagc gaagcaacaa tggattgtgt
     7141 tgctgcggtt ttatagggta ggggagaggc agatgcgaat taaaccagac gataactggc
     7201 gttggtatta cgatgaagag cacgatcgaa tgatgctcga tttagccaat ggtatgctat
     7261 ttcgctcacg ttttgcgcgc aaaatgttga ctccagatgc cttttctccc gcaggctttt
     7321 gcgttgatga tgccgcgctc tatttctctt ttgaagaaaa gtgccgcgat tttaatttat
     7381 ctaaagacca aaaagccgaa ctggtgctga atgcactggt agcaattcgt tatctcaaac
     7441 cgcaaatgcc gaaaagctgg cattttgtct cccatggtga aatgtgggtg cccatgccgg
     7501 gcgatgcggc ctgcgtttgg ttgagcgata ctcacgagca ggtcaatttg ctggtcgttg
     7561 aatctggcga aaatgccgcg ctatgcctgc tggcacaacc ctgcgttgtt attgcgggtc
     7621 gcgcgatgca gttgggcgac gccattaaaa tcatgaacga caggctgaaa ccgcaggtga
     7681 atgttgacag cttcagcctc gaacaggcag tctaacgcgt cagcttaagg gcagtcttcg
     7741 gtacacagct acagcaaaga atggtgccat catcgcccat tgctgatttt ttcagcggcg
     7801 tgacttcgcc ttctaaaagc tgaacacggc aacttccaca aatgcccgcg cggcaagaat
     7861 aagggatacg aattccctga ttttctaatt gctccagcaa cacttgttgg ttatttccac
     7921 gaaatgcctg tccctgccag tcaatatcta catttgcgtc cggttgttgc gtgatgttgg
     7981 cggtatcatc agcggcagct gcgccgtaaa tttttgccgg agccgttgcc agaatttcca
     8041 cctcatcgcc aacgcgaatc acgccgctat tacgggcaat taaattctga ccaaaatcga
     8101 catcgccgtt atcctgggca gtgcggaaag attgcaatgt ttttaatggt tcgcctgccg
     8161 gatgtttttg ccctttttct gggctgacgg tggtgaaaat acagcggcta caaggtttaa
     8221 ccacatcaaa caccacatca ccaatgcgaa tcactttcca gctatcttct tcccaggctg
     8281 acgcgccgga aaccaccaga ttggggcgga attgctccat ttttacactg gccggacaac
     8341 gttgttggag atcacgtaac gaggcttcgt tagcaagaag gtaaggatag ccatcagcaa
     8401 atgacagagg tacagtgttg tggcgtttca cgcgccgggt catttgtggc cccacccagc
     8461 gtaattgcac ttcgcgggag aaaaatccac ttagccattt gttgatcgcg tctggcgcaa
     8521 ttcgcgcggt aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag
     8581 caaaacgaac atatgcgcta ctgccatctg gtgcggtgag atgcaagcca tcatgcacag
     8641 gtgaaggagt aaaccgtacc atctggggaa actggcgagc ggtaataaac gtaccgtcag
     8701 gttccgtgat cataaagatg cgatcgaagg ccagaccact gacatcagcc agagcatgtg
     8761 taagaccaat gccgcgcatc gatttaacag gatgaataaa aagccgggtt aatgtcgcca
     8821 cggtgcggtc ctcaaatgaa aataagccct caactttatg acatgacgcg cttattagct
     8881 ataatgcgca acaattttct tagcgcctgt cccgttaggc tattttattt gtcagtttgg
     8941 ccctgggttg tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct ccgcgctctc
     9001 catgtccaga ctgactgcaa caattacgcc tactgcggta ggctcttaga gtaaaagtga
     9061 cgatatgaat tctctgtttg ccagtacggc ccgtgggctg gaagagctgt taaaaactga
     9121 actggaaaat ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc atttcaaggg
     9181 cgacacacgg cttgtttacc agagcctgat gtggagccgc ctggcctcgc gtattatgtt
     9241 gccgctgggc gagtgtaagg tttacagcga tttagacctc tatctcggtg ttcaggcgat
     9301 caactggaca gagatgttta atcctggcgc gaccttcgct gtccacttca gtggtttgaa
     9361 tgacaccatc cgcaacagtc agtacggcgc gatgaaagtg aaagacgcga tcgtcgatgc
     9421 tttcacgcgg aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg atatccgcgt
     9481 taacgtctgg ctgcataaag aaaccgccag tatcgctctt gatctgagtg gtgatggttt
     9541 acatctgcgt ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa ccctggcagc
     9601 cgctattgtg atgcgatccg gctggcagtc aggaacaccg ctgctcgatc cgatgtgtgg
     9661 ttccggtacg ttgctgattg aagcggcgat gctggcgacc gatcgcgcac caggcttgca
     9721 ccgtgggcgt tggggcttta gcggctgggc gcagcatgat gaagctatct ggcaggaagt
     9781 gaaagcggaa gcgcaaactc gcgcccgtaa aggcctggct gagtatagct ctcattttta
     9841 cggttcggac agcgacgcac gggtgattca acgtgcgcgc actaacgccc gtcttgcggg
     9901 gattggtgaa ctgatcacct ttgaggtgaa agatgtcgcg caactgacca atccgctgcc
     9961 gaaagggccg tacggtacag tgttgagcaa cccgccatac ggtgaacgtc tggacagcga
    10021 accggcgctg attgcgctgc atagtctgct gggccgaatc atgaaaaacc agttcggtgg
    10081 ctggaatctc tctttgttta gtgcctcgcc ggatctgcta agctgcctgc agctgcgtgc
    10141 agacaaacag tacaaggcga aaaacggccc gctggactgc gtacagaaaa attaccacgt
    10201 tgccgaaagc acaccagaca gtaaaccggc aatggtagcg gaagattacg ccaaccgcct
    10261 gcgtaagaac ctcaaaaaat tcgagaagtg ggcacgccag gaagggattg aatgttaccg
    10321 cctgtatgac gccgatctgc cggaatataa cgttgccgtt gaccgttatg ccgactgggt
    10381 ggtggtgcag gagtatgcgc cgccaaaaac tattgatgcg cacaaagcgc gtcagcgtct
    10441 gttcgatatt atcgctgcaa ccatttcggt actggggatt gcgccaaaca aactggtgct
    10501 gaaaacccgt gaacgccaga agggcaaaaa ccagtaccag aaactgggcg agaagggcga
    10561 gtttcttgaa gttaccgaat ataacgctca cttgtgggtg aacctgacgg attatctcga
    10621 tactggtctg ttcctcgatc accgtatcgc ccgccgtatg ctcggtcaga tgagcaaagg
    10681 caaagatttc ctcaacctgt tttcttatac cggcagcgcc accgtgcatg cgggattagg
    10741 cggtgcacgc agcaccacca ccgtggatat gtcgcgtact tatctggaat gggcagaacg
    10801 caacctgcgt ctgaatggcc tgactaggcg tgcgcatcgc ctgattcagg ccgattgcct
    10861 ggcgtggctg cgtgaggcaa atgaacagtt cgatctgatc tttatcgatc cgccaacctt
    10921 ctctaactca aaacgaatgg aagatgcgtt tgatgttcag cgcgatcatc tggcgctgat
    10981 gaaagatttg aaacgtctgc tgcgtgcagg tgggacgatc atgttctcga acaacaaacg
    11041 tggcttccgt atggatctcg acggcctggc aaaactggga ctgaaagcac aagaaattac
    11101 gcaaaaaacg ctctcccagg atttcgcccg taaccgccag atccacaact gctggctgat
    11161 taccgcagcc tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc atggctgtcg
    11221 ttcagcgacg cgccgcttct cgataacgca gaactgcata tcgaagataa cgaacgtgtt
    11281 tgtctggtgg gccgcaacgg cgcaggaaaa tcgacgttaa tgaaaatcct caaccgtgaa
    11341 caagggctgg atgacggtcg cattatttat gagcaagatt tgattgtagc tcgtctgcaa
    11401 caggatccgc cgcgtaacgt tgagggtagc gtttatgatt tcgttgccga aggcattgaa
    11461 gaacaagcgg aatatctgaa acgctatcac gatatttcgt gcctggtgat gaacgacccg
    11521 agcgagaaaa atctcaacga actggcgaag gttcaggaac agctggatca ccacaacctg
    11581 tggcagctgg aaaaccgcat caacgaagtg ctggcgcaac tggggttaga tcctaacgtt
    11641 gcgctgtcgt cgctttccgg cggctggttg cgtaaagcgg cattaggacg cgcgctggtg
    11701 agcaatccgc gcgtattgct gctggacgaa ccgactaacc atctggatat cgaaaccatc
    11761 gactggctgg aggggttcct taaaaccttc aatgggacga ttattttcat ctcccacgac
    11821 cgttcgttta tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg caagctggtg
    11881 acctatccag ggaattacga ccagtacctg caggaaaaag aagaagccct gcgcgtggaa
    11941 gaactacaaa atgccgagtt cgatctcaaa ctggcgcagg aagaagtgtg gattcgctag
    12001 gggatcaaag cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa agcgatgcgt
    12061 cgcgaacgtg gtgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt ggaagaggcc
    12121 agccgctccg gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt taacggtaag
    12181 caactggtga aagatttttc tgcccaggtt ctacgtggcg acaaaattgc cctgattggt
    12241 ccgaatgggt gcggcaaaac cacgctgctg aaactgatgc tcggtcagct tcaagcgggc
    12301 agcgggcgta ttcacgttgg caccaaactg gaagtggctt atttcgatca gcaccgcgcg
    12361 gaactggatc ccgataaaac ggtgatggat aaccttgccg aaggtaagca agaggtgatg
    12421 gttaatggca agccacgcca cgtattgggc tatttgcagg actttctgtt ccatccgaaa
    12481 cgggcgatga cgccagtacg tgcgctttct ggcggtgagc ggaaccgctt gctgctggcg
    12541 cgtttgttcc tcaaaccaag caacttattg attcttgacg aaccgaccaa cgatcttgat
    12601 gtcgaaacgc tggaactgct ggaagaactg atcgacagct atcagggcac ggtattgctg
    12661 gttagccacg atcgtcagtt tgtcgataac accgttacag aatgctggat cttcgaaggc
    12721 ggcggtaaaa ttggtcgtta tgttggcggt tatcatgatg cccgtggtca gcaagaacaa
    12781 tatgtggcac tcaaacagcc tgcggtgaaa aaaagcgaag aagccgccgc gccaaaagca
    12841 gaaactgtaa aacgcagcag tagcaaacta agctataaat tgcagcgcga actggagcag
    12901 ctaccgcaat tgctcgaaga tctggaggcg aagctggaag ccctacagac gcaagtggcg
    12961 gatgcttcct tcttcagtca gccgcatgag cagacgcaaa aagtgcttgc tgatatggct
    13021 gctgcagagc aggagctgga gcaagccttt gaacgctggg agtatcttga agcgttaaaa
    13081 aatggtggct gatcgcaaaa tagtcgattt aaacagagac agctgacaac ggtaaaattg
    13141 cctgatgaac tgggtttatc aggcatgaaa accaacactt tgttagtcat ctgtaaaata
    13201 gcgcatcatt aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca catcctgtgc
    13261 tcgcagtgtg acatgctggt ggcgttaccg cgccttgagc atggtcagaa agcggcatgt
    13321 ccccggtgtg gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg tcccaccgcc
    13381 tatgcgttgg ctgcactgtt catgctgttg ctgtccaact tgtttccttt tgtgaatatg
    13441 aacgttgcag gagttaccag tgaaattaca ttactggaaa ttcccggcgt gcttttttct
    13501 gaggactacg ccagcctcgg cacctttttc ctgttgtttg tgcaactggt tcccgcgttt
    13561 tgtctgataa ccattctgtt actggtgaat cgcgcggaat taccggtccg tttaaaagag
    13621 caactggcac gggtgctttt tcaactcaaa acctggggaa tggcggagat tttcctcgcg
    13681 ggtgtgctgg tcagtttcgt taaactgatg gcttacggca gcattggggt aggcagcagc
    13741 tttctcccct ggtgtttatt ttgtgtcctg caactgcgcg cttttcagtg cgttgatcgt
    13801 cgctggttat gggacgacat cgccccgatg ccagaactgc gccagccgct aaaaccaggc
    13861 gtcacgggga tacgtcaggg gctgcgttcg tgctcctgtt gtacggcaat ccttcctgct
    13921 gatgaacccg tgtgcccgcg ttgtagtacc aaagggtacg ttcggcgtag aaacagcctg
    13981 cagtggacac tcgcgctgct tgtaacgtcc atcatgctgt atcttccggc taatattttg
    14041 cccatcatgg tgacggattt attaggctcg aagatgccgt cgacgattct cgctggggtc
    14101 attctgttat ggagcgaagg ctcttatccc gtcgctgtgg tgatctttct ggccagtatt
    14161 atggtgccaa cgttaaagat gagcgccatc gcgtggctgt gttgggatgc caaagggcat
    14221 ggcaagcgcg acagtgaaag aatgcatttg atttatgaag ttgttgagtt tgtaggccgc
    14281 tggtcgatga ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg tatgggaggt
    14341 ttaatgagta tttatccggc aatgggtgca ttaatgtttg ctttagtcgt cataatgaca
    14401 atgttttctg ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc tgaatcagag
    14461 catgaggagt cctgacagta tggaatctaa taatggggaa gccaaaatcc agaaagtgaa
    14521 gaactggtct cccgtgtgga tatttcctat cgtcacggcg ctcattgggg cctgggttct
    14581 tttttatcat tacagccatc agggaccgga agtgaccctg atcaccgcga atgcggaagg
    14641 aattgaaggt ggcaaaacca ccattaaaag ccgtagcgtt gacgtcggcg tggttgaaag
    14701 cgccacactg gctgatgatt tgacgcacgt tgaaatcaaa gcgcggctga attccggtat
    14761 ggaaaaattg ctgcataaag acaccgtctt ttgggtggtg aaaccgcaga ttggtcgcga
    14821 agggattagc ggcctgggaa cgctgctgtc tggagtttat atcgaactgc agccaggcgc
    14881 gaaaggcagc aaaatggata aatacgattt gctggactca ccaccgttgg ccccgcctga
    14941 tgcgaaaggt atccgtgtga ttctcgatag caaaaaagcc gggcagctct cgccaggaga
    15001 tccggtgctg ttccgtggct atcgggtagg ttcggttgaa accagcacct tcgatacgca
    15061 aaaacgcaat atcagttatc aactgttcat caatgcacct tatgaccgac tggtgaccag
    15121 caatgttcgc ttctggaaag atagtggcat tgcggttgat ctgacgtcag cggggatgcg
    15181 tgtggagatg ggctcattga caacgctgct tagtggcggt gttagctttg atgtgccgga
    15241 aggtctggat ttagggcagc cagtggcacc gaaaacagct ttcgttttgt atgatgatca
    15301 gaagagcatt caggattcgt tgtacaccga tcacattgat tatctgatgt tctttaaaga
    15361 ttcggtacgc ggtctgcaac cgggagctcc ggtagaattc cggggtattc gcctgggtac
    15421 cgtaagcaaa gtgccattct ttgcgccgaa tatgcgtcag acatttaacg atgattaccg
    15481 tattccggta ctgattcgta tcgagccaga gcggctgaaa atgcagcttg gcgaaaatgc
    15541 ggatgttgtt gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat cgctgaaaac
    15601 cggaaacctg gtcactggtg cactgtatgt tgatctcgat ttctatccaa atacgcctgc
    15661 aataaccggt attcgtgaat ttaatggtta tcagattatc ccgactgtta gcggcggcct
    15721 ggcgcaaatc cagcaacgac tgatggaagc gttggataag atcaacaaac tgccattgaa
    15781 tccgatgatt gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa tgaaaaacct
    15841 gcaaacgacg ctggatagca tgaacaagat cctcgccagc cagtcgatgc agcagttgcc
    15901 gacggatatg cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct tccagcctgg
    15961 ctccgcagcc tacaacaaga tggtggcgga tatgcagcgc cttgatcagg tgttgcgaga
    16021 actgcaaccg gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat ttgaagcgaa
    16081 ggacaaaaaa gatccagagc cgaagagggc gaaacaatga aaaagtggct agtgacgatt
    16141 gcagcactgt ggctggccgg atgcagctcc ggcgaaatta ataaaaacta ttaccagtta
    16201 cctgtggtgc agagcggtac acaaagtacc gccagccagg gcaatcgtct gttatgggta
    16261 gagcaggtca ctgttcctga ctatctggcg gggaatggtg tggtttatca aaccagtgat
    16321 gtgaagtatg tgattgccaa caacaacttg tgggccagcc cgttggatca acagttgcgc
    16381 aacaccctgg ttgccaacct gagtacgcaa ctgcccggct gggtggttgc ctcccagcct
    16441 ctgggaagcg cccaggacac gctcaatgtt accgtaacgg agtttaacgg tcgctatgat
    16501 ggcaaggtca ttgtcagtgg tgagtggctg ttgaaccacc agggacaact gatcaaacgt
    16561 ccgttccgtc tggaaggagt gcaaactcag gatggttacg atgagatggt taaagtgctg
    16621 gccggtgtct ggagtcagga agccgcttct attgcacaag agataaagcg tctaccttaa
    16681 ttataaagat ttgtaaatat aaccgtctcc ggtatgccgc ctgaggcggt ttttttgttt
    16741 ctaacgtgcg gaaaaatttg ttcctcttca cattttttgt acaaccgaca tgcccgtgta
    16801 gctcacaaat atgacagtgg cgtgaatttt gcgcattgac ggcagttatg attcgcggta
    16861 ttgcttaact gtgattgcac atttagtaat cactgttttc ttttccacca gaaaccagta
    16921 tgagggaaac gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc acatcaacgt
    16981 ggttatcagg ccggcatcgc cggacgctca aaagaaatgt gtccctatca gacgctgaat
    17041 caaaggtcac aatggctggg aggctggcga gaagccatgg cggacagggt agtaatggcc
    17101 tgattctgtc tctttaaaaa gaaacctccg cattgcggag gtttcgcctt ttgatactct
    17161 gtctgatgat aatcagaagg cagacgtatc ctggaacaga ccgactttca ggtcgctggc
    17221 ggtatagatc agacgacaat caaccagcac ttcgccatct gccagaccca taatcagacg
    17281 acgattaaca atgcgtttaa agtgaatacg gtaggtcact tttttcgctg tcggcagtac
    17341 ctgaccagtg aatttcactt cgccaacgcc cagcgcgcgg cctttacctt cgccgcccag
    17401 ccagccgagg tagaacccta ccagctgcca cattgcgtcc aggcccaggc atcccggcat
    17461 aaccggatcg ccaatgaagt ggcatccgaa gaaccacaga tccggattga tatccagttc
    17521 tgcttcaaca taccctttgt cgaagttacc acccgtttcg gttattttga ccacacggtc
    17581 catcatcagc atgttcggtg ctggcaattg cgggccttta gcgccaaaca gttcaccgtg
    17641 accagaggca agaaggtctt cttttgtata ggattcgcgt ttatctacca tgttctctgt
    17701 aagccttatt ttattgaagc acgcaggata gctaacacgt gtacgctgaa caagtccgat
    17761 cagttcggaa taaaccagtt cagccaacgt aatggccatg gaaaacggtg acgtccttcc
    17821 tgttgcgatg cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt ttggccttcg
    17881 ccatcccaca ccagatttaa taataacggc agtgcgtcag tcacatcgtc tactgcccag
    17941 atggtgaatt tgccttcttc taccgctttc accagttcac tgtgaagact taaatggcga
    18001 acgttagcag tggggataat gacaccttgt ttccccgtta actcacgttg ctggcaaata
    18061 gcaaagaagc cttcgatttt ctcatttaaa ccaccgaccg gctggacgcg gccgaactga
    18121 tcgactgaac ctgtgatagc gatactctga ttcactggca catcagcgag ggcgcttatc
    18181 agggcgcaga gttcagccat cgaggcacta tcgccatcca cttcactgta tgactgctca
    18241 aatgtcagcg atgctgagaa ggggatctgt tgctcaagct gtagttccga catcaggaac
    18301 gcctgcatga tcatcatccc tttcgcatgg atattgccgc caagctccgc tttgcgttcg
    18361 atgtcggtga attcaccatc gccaatatgc acaacgcagc taatgcgaga aggttcgcca
    18421 aaagcgcgtg gatgacccgg aaattcaatg accgaaaggg cgttaatttg cccgatgcgt
    18481 tcgccttcgg tttcaatcag gatttgctcc tgaaggatct catcctgcat acgttcagcg
    18541 aggaaacctt cgcgccattc acgctgctgc agcattaaat ttagctgctc gccggagaag
    18601 gtgtcgccat cacacaggga ggcgacctct ttacactggc ggaggatcca atgcgggcta
    18661 agcggaagtg tttcttgttc accggtgtag cgtgctgctt cgcggataag taccggccaa
    18721 gcatccgctc ccggggcagg taagtgatta tgtctggcgg taaatgtcac ccagcgacac
    18781 cactggctta ctgactccgc atcgacaatc tgcagagtat cttcaaattc gctataaata
    18841 gcctgctctg aaagctctgg ctccatctcc tggaaatcag ccaatgattc gcgttcgcct
    18901 accagaatga ctttcagctt caatggcatc gaaggcacag agacggggag agggcgcgac
    18961 tcatcaaacg caacccagtc aaaacgctcg cggttaacga tatttttcag ccgcatccac
    19021 agcagaggtt gcgccagcag tgtacgcaaa gagatgatga gaataccgcc atttgcctga
    19081 tgcaccagac caggctgcag ggtaatgtcg ccattaaact ggcgcaggca gccaaagagt
    19141 tgctccgctt ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa attatcatct
    19201 gcactcactg cgtgacgtaa gcggatggag tggccggaaa cctcatagtg atcgcccacc
    19261 agttggcctg catcgctttg tagcgtacgc gcggcatcgg caataagatt cagatactca
    19321 gactcttccg gggccttagc cagcataaaa gaggaggatg ctcgcgtatg cagcaactgc
    19381 tccagcgcaa attgcagtcg cggttgagta tcactgaata aaggatcgtt ttcgtcaatc
    19441 aaatgtggct gagcaaatat ttcctgatag ctatcggtat caggaaccag gtcacgccat
    19501 gcaagtttcg taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc cgcgattata
    19561 ccgtaaccgg cactacagca cacgtagaaa gcaccgacaa tactcctggc tgggcgttaa
    19621 agctcacagg atggagattc ttttcttcac tggcctaaaa agctgatatt ctgtaaagag
    19681 ttacacggta acattgagat cgctatgaaa tatcaacaac ttgaaaatct tgaaagcggt
    19741 tggaaatgga agtatctggt aaagaagcat cgcgaagggg agttaattac ccgttacata
    19801 gaagccagtg ccgcccagga agccgtcgat gtgttgctct cgctcgaaaa tgaacccgtg
    19861 ctggtaaatg gctggattga caagcatatg aatccggaac tggtcaatcg catgaagcag
    19921 accattcggg ccagacggaa gcgccatttt aatgcggaac accagcatac gcgcaaaaaa
    19981 tcgatcgatc tggaatttat tgtctggcaa cgtctggctg gtcttgcaca gcgtcgcggt
    20041 aaaacgcttt ctgaaacgat tgttcagctg attgaagatg cggaaaacaa agagaaatac
    20101 gcgaataaaa tgtcttctct gaagcaggat ctgcaggcat tgctgggtaa ggaataactg
    20161 acgaaagtca gttcaattta ctaaaggcaa aaaaaacccc gcagcagcgg ggtttttcta
    20221 ccagacgata acttaagcct gcggctgagt tacaacgtct ttgatacctt taacttcgat
    20281 ctctacgcga cgatccggag ccaagcagtc gatcagtgca gcacgctgtt tcacgttgtc
    20341 acaggtgttg ccagtaaccg ggttggattc gcccatacca cgtgcggaga tcttgtctgc
    20401 cgggatacct ttggagatca ggtaatcaac aacagactga gcacggcgct cggacagacc
    20461 ctggttgtaa gcgtcagaac cgatgcggtc agtgtaaccc agaacaacta cggaaccgtc
    20521 tttcggatcc aggttgctca gctggctgta cagctgatcc agagcagcct gaccttccgg
    20581 tttcagggtt gctttgttga agttgaacag aacgtcagac ttcagagtga agtgcttggt
    20641 ctgtacttcc ggtgccggag ctggagcaac taccggagct gcttcgccct gaccgaaacg
    20701 gtaggaaaca cccaagctca gcagaccgtt gtccggacga gtaccgatgg tgtttgcgtc
    20761 accgatgttg ttggtccact ggtattccag acgggtagcg atttcaggag tgatcgcgta
    20821 ctcaacacca cctgcgaaaa ccggagaaac gccagtgtcg tggtctttaa aggatgcgcc
    20881 accaggtacg ttagccttgg tgtctgcacg ccataccata ccacccagac gagtgtagat
    20941 gtccagatcg tcagtgattg ggtaacccag tttagcggtc agctgaacgc cctgagcttt
    21001 gtatgcgccg ttgatgttgt cgcctttgta cggcatacga cctaaccagt cgtaacccat
    21061 ttcaaaacca acatacgggt taacctggta accaccaaaa gcacctgcac ccagttggtt
    21121 ttcgtgggtc ggaccattgt taggaataaa accagtgtca tggtactggg accagcccag
    21181 tttagcacca gtgtaccagg tgttatcttt cggagcggcc tgcgctacgg tagcgaaacc
    21241 agccagtgcc actgcaatcg cgatagctgt ctttttcatt ttttgcgcct cgttatcatc
    21301 caaaatacgc catgaatatc tccaacgaga taacacggtt aaatccttca ccgggggatc
    21361 tgctcaatat taactctacc gatatcttcg gcttatgccg agcacccctg gcgatgtaaa
    21421 gtctacaacg tagttgaaaa cttacaagtg tgaactccgt caggcatatg aaaaaaaagt
    21481 cttgtataag gtatgtttaa tctttttgtc agcgacaatt tacagaaaaa aaacgcggaa
    21541 accgcttcag acaagccttc gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg
    21601 acaaaaaaag ttccaggatt aatcctaaat ttacttaatg atacaaatta gagtgaattt
    21661 ttagcccgga aagttgtctc gttgtgtgag aggatgcgct taccggacgc ataataaacc
    21721 ccatagcgtt accttcattt gccgcatcaa caagttcagc atgctcttct tcagtcaaat
    21781 catctgtcaa ccaaccgatc accacactgt aattgcccgt gcgtaaagcg cgaaccattg
    21841 actccactgt gtggcaaggg gagagctggc taatctgcat tactttcgtt aagggtagcc
    21901 cagatgcctg aacccattcc cgactcagtt tttgttgcgg tgttaaccag agttgccagc
    21961 gcgattgctg accgagttgc tgtaacaatg gcaacagtag aagttgcgtc atcatgggct
    22021 gatcttcgcg atagacaact tcactgataa gcccggctgt agtgttttcc gtagagacac
    22081 gcgcaatttt acttgctgcg gatgagaacg acgaagaacg atgtgcatag cctgaagtgt
    22141 acataatcaa tccagcccct gtgagttact gtatggatgt gcagtacatc cagtgacaac
    22201 aaagatcaac cctattttcg gaaagagcct cgcaaatttt gtcgttggtg acgggaaaac
    22261 ataaattaat cttgcccctt aagaataagt tgcctatttt cgtagttaac ggatccgtta
    22321 atgtgaatca ttcttttatg ttatgatttt aaaaggaatt ttatgaaaag cctctcctat
    22381 aagcggatct ataaatcaca agaatacctg gcaacgttgg gcacaattga ataccgatca
    22441 ttgtttggca gttacagcct gaccgttgac gacacggtgt ttgcgatggt ttctgatggt
    22501 gagttgtatc ttcgggcttg tgagcaaagt gcacagtact gtgtaaaaca tccgcctgtc
    22561 tggctgacat ataaaaagtg tggccgatcc gttaccctca attactatcg ggttgatgaa
    22621 agtctatggc gaaatcaact gaagctggtg cgtctgtcga aatattctct cgatgcagcg
    22681 ctgaaagaga aaagcacgcg caatacccgg gaaagactga aagatttgcc caatatgtct
    22741 tttcatctgg aagcgattct cggggaggtg gggattaagg atgtacgggc gttacgtata
    22801 cttggggcaa aaatgtgttg gttgcgactg cggcagcaaa acagtctggt gacagaaaag
    22861 attctgttta tgcttgaagg tgccattatc ggcattcatg aagctgcgct cccggtggca
    22921 cgccgccagg agcttgcaga atgggctgac tctcttacgc cgaaacagga gtttcctgcg
    22981 gagcttgagt aatctggcgt tgcagacgac caatctctgg cagtaatgca atcaataacc
    23041 caacttgttg tacgaccaga ggttctttgc tatctgcccg tggttcaagt tgctgcatcc
    23101 gctgtttcag gccagctaat gcctgattga cgcgttcttc atcagcaggt tgatgatgta
    23161 acgcgtcatc aacatagcaa actgcgtcat caagaaacgc cagaatttca ggattagtta
    23221 actgctcccg gtgagcaccg agggctgaga tatagctggt aaacgtatgg ttaaggcaca
    23281 gcaagcgaaa cgcggcttcg cgaatttgcg gggtaacgtt cggttcgctg gacatatttg
    23341 ataccacaga cgccagttca gcatcacggt tgtgtgcatc gcggcgggca atacgatacg
    23401 ccagacggtt atcacgcccc tgatggtatt gctccagtat ggcatcgaga tagcgacagt
    23461 tggcctctgt ggcgcgttcg agcattcgcg gcagattgcg aaacttccag tcgggccaga
    23521 tgtagctcac tgccgcccac gcaatggcac aaccaatcag cgtatcgatt acgcgaggta
    23581 acgctacttc aaaaccttca cccagtaagt taaaacacag tagcaccaaa agtgtgatga
    23641 acatcgttgc atgagcgtat tgcacgttac ggaaggcaaa aaagagcacg ccggtaataa
    23701 ccagcagcac cagctgccct tccagtgatg gcacaaacca cagcacagga atgccaatcg
    23761 cgatacctac cagcgtacca ataatcctta acttcaggcg gtggcgcgtg gcgttatagt
    23821 ttggctggca gacaaacaaa cttgtcagca agatccaata cccgtgatgc attccggtta
    23881 tttgaatgat ggcgtagccg aagcacaaca ccagcgacat tcttaccgca tgacggaaga
    23941 gggcggattc cggcgtgaag tgacggctaa gacgcagcca gatatcactc aatccgtgcg
    24001 ggctgtcatc agcgagctca ttttcgtcat tattacgggg tagtgcctgg gcctgttctg
    24061 attcaattgt tgccagttgg gcatcaatgg cgcgtaaatt gttcagcaaa aatcccagtg
    24121 ttttgagtaa atcggcgggt gcgccgttat cgcgtatccg ctccagcgca gcatcaatat
    24181 gcgtaaaagc gcgctcaaaa tgcggatcat gttgataaga ctgacgcaac aaaatacagc
    24241 gtgacagttg ctggcacgcc tggccctgca tcgacatcag ccgctgaaaa cggaacagca
    24301 cgtcgctgtg gcgaaaatgt tcacgcaatg tttgatactg aatatgagaa gagctggcac
    24361 gctcgtgaat atcctgtgcg acaaagtaat aatgcagcgt gcgacgcgtt ccccgttgac
    24421 cacgatcgcc acgtaagcgg gtcagcagcg agagtttcgt ctgattcaat gtcgccatca
    24481 gctgaccgtt ggcgagagcc aaatcgtaca gcggtgcctg gctttcatct tcaatatcag
    24541 gatcaaacat gcgcgacttg agctcaagat aacgcgccag ttgttcatag caacgcgcca
    24601 ggttgtcctg cagcgggcgg accgggaaca gcagatgacc aataagtgtc aggacgttgt
    24661 accagacggc accggccagc agatacatcg gctgctgata ccagtgctca tacagtgatg
    24721 ttcccaacat agtgtaaatg gcgatcagca atgcaccgaa ggcaattgtt gcatagcgtt
    24781 gacccagacc gccgagcaaa atgaagccgc tggtagagag cgttaagcca atcgcaaata
    24841 gccagggcca gggaaacagc aattctactg aggccgaggc gataaaaaag cagaacagcg
    24901 taatgatgag gttacgcaaa cgtcccgcca gtcggtcatc gagatcggtc agcgccgctg
    24961 ccaccatccc cagcgttagc ggaatcgtca gttttacatc acccagccac cacggaaacg
    25021 ctgtggttcc acaaagcgca ataaaaatac gcgcgtaata cagccaggcg ctgttccaag
    25081 tatagcgttt gagcaaagga cttagcataa aggccatatt agtggattat tcaaaacgac
    25141 gacgtgcatt ggcttcacgc gcagcttgcg ctgtttctac cgataccacg cgacgaccaa
    25201 ccggccatag tgcaatagcg gcaattttaa agttcgcaat gccgacagga atgccaatga
    25261 ttgaaataca ttgtgcgatg cccgttgcaa tgtgcatcag gcataaccac cagccaaaga
    25321 aaatcagcca gaaaatattc aataccgtac cgccagtatt cagcaacaca tttttgccag
    25381 ccgggttcag ttcatcgaca tgaatagctt cattgccata aggcaccaga gacagtttag
    25441 tgatctccca gcaggatcgt gtcagcggta aggtaaaaat cagcacaata ctgaccagag
    25501 tcgccaacag ccagcccaga gtggtggcaa atccgccaag cacaaagttc agaatgttca
    25561 aaacggtacg cataaaacct cgctttactg tggttttcag taatgggcgg caattgtaac
    25621 gtttttttgg gctggagcac gttttctctg acggttacac tgataagaaa taatttcgtg
    25681 tggatctaca gagtcatgga actgaaagcg acaacgcttg gaaaacgtct ggcacagcac
    25741 ccttacgatc gggcggtgat cctcaatgcc gggattaaag tctccggcga tcgccacgaa
    25801 taccttattc ctttcaatca attactggcg attcactgta agcgcggtct ggtatggggc
    25861 gagctggaat ttgtactgcc ggacgaaaaa gtggtgcgtc tgcacggcac agaatggggc
    25921 gagacgcagc gtttttacca tcatcttgat gctcactggc ggcggtggag tggcgagatg
    25981 agcgaaattg cgtctggtgt tttacgccag caactggatt tgattgccac gcgcactgga
    26041 gaaaataaat ggctgacgcg tgagcaaacc tctgacgttc agcaacaaat ccgccaggct
    26101 ttgtcggcgt tgccgttgcc ggttaaccga ctggaagaat tcgataactg ccgtgaggcg
    26161 tggcgtaaat gtcaggcttg gttgaaagat attgaaagcg ctcggttgca gcataaccag
    26221 gcgtataccg aagccatgct taccgagtat gcggattttt tccgccaggt cgagtcttca
    26281 ccgctgaatc cggcgcaggc ccgggcagtc gttaatggcg agcattctct gttagtgctg
    26341 gccggtgcag gaagcggaaa aacgtcggtg ctggtggccc gtgcaggctg gttgctggcg
    26401 cgtggtgaag cgtcccctga gcaaatttta ttgctggcgt ttggtcgcaa agccgctgaa
    26461 gagatggacg agcggattcg cgaacggcta cataccgaag acattaccgc acgcacgttt
    26521 catgcgctgg cgctgcatat tattcagcag ggcagcaaaa aagttccgat agtcagcaaa
    26581 ctggaaaatg ataccgctgc ccgtcatgaa ctctttattg ctgagtggcg caagcaatgc
    26641 agcgaaaaga aagcgcaggc gaagggctgg cggcaatggc tgacggaaga aatgcagtgg
    26701 tcagtgccag aaggtaactt ctgggatgat gaaaaattac agcgtcgcct tgcctctcgc
    26761 ctcgatcgct gggtaagtct gatgcggatg cacggtggtg cacaggcaga aatgattgcc
    26821 agtgcacccg aagagattcg cgatctgttc agtaaacgta tcaagttgat ggccccgtta
    26881 ttaaaagcct ggaaaggtgc gctgaaggca gaaaacgctg tcgatttttc gggccttatt
    26941 catcaggcga ttgtgattct ggagaaaggc cgctttatca gcccgtggaa gcatattctg
    27001 gttgatgaat ttcaggatat ctcgccgcag cgcgcagcgt tgttagcggc attacgcaag
    27061 caaaacagtc agacgacgtt gttcgccgtt ggtgatgact ggcaggcgat ttaccgattc
    27121 agcggtgcgc aaatgtcgct caccaccgct ttccatgaaa actttggtga aggcgatcgc
    27181 tgtgatttag acacgactta ccgttttaac agtcgtatcg gtgaggtggc aaaccggttt
    27241 attcagcaga acccaggcca gctgaaaaag ccgctaaaca gcttaaccaa tggagacaaa
    27301 aaagctgtca cgttattgga tgagagtcaa cttgacgctt tgctggataa gctctctggt
    27361 tatgccaaac cggaagagcg cattctgatc ctggcgcgtt accatcacat gaggcctgcc
    27421 agcctggaaa aagcggcaac acgctggccg aagttgcaaa tcgactttat gaccattcat
    27481 gccagcaaag ggcaacaggc ggattacgtc atcatcgttg gcttgcagga ggggagtgat
    27541 ggttttccgg ctgcggcgcg ggagtcgatt atggaagagg cgctactgcc accggttgag
    27601 gatttcccgg acgctgaaga acggcgttta atgtacgtgg cgctgacccg ggcacgccat
    27661 cgggtatggg cactgtttaa caaagagaat ccctctccct ttgtggaaat actgaaaaat
    27721 ctggatgtgc cggtggcgag aaaaccgtaa gaaacaggtg gcgtttgcca cctgtgcaat
    27781 attacttcag acggtcagcg agataacgct gataatcggg gataagaata tcgaccgcgt
    27841 cgttgaaatg cggcgactgg ataatgaagt ctgccgttgc cacgttggtg gcgaccggaa
    27901 tgttccatac cgtcgccaga cgcagcaagg ctttcacgtc aggatcgtgc ggcacggcat
    27961 ttagtggatc ccagaagaaa atcaatacat caattttccc ttctgagatc aatgcgccaa
    28021 cctgctggtc accccccatt gggccactca acatcgcgtt gacgttcatg ccggtcgcgc
    28081 gggaaatcaa gttaccggta gtgcctgttg catacagtac gtgttgttcc agtaacggtt
    28141 gatgccgttc cacccagctc atcagcattt gtttgcagtg atcgtgtgcc accagcgcaa
    28201 tatgtttccg cgcaggtaaa gtgcgagtcg tcagttccat aatgtacatc cgtagttaac
    28261 tttcctacag attactgtaa gcacttatcg ctgcaagata aagaccgaaa aagcctgcgc
    28321 acaggcacaa aaatctcagg aagatggttg tttttccgcc cattgcagga aagtatttcg
    28381 cgtttgtggg tcagccagtt taaaccaata cttcagccgt tgttctgtga gcacctgaga
    28441 ctgcggtgga atagcatcca gctcggaaac acccgaaagc agcgtactgt catctgccat
    28501 catcgtggca aattgcggta gtgaagcgcg tttcgcggat ttgttatagc gttcaacctc
    28561 gacctcataa tcaatcgttt ttgccgttga ggtaatggcg agaatatcca gctttaccgg
    28621 aatcggcgtc gcatcgccat ccaacaattc aaggcgcggc gcggcatcaa aatggttagc
    28681 ttcccgctca ttctccaggc gaggcaggcg aaaattgacc tggttgatga gctgggtatt
    28741 aaaactgacc accagcggtg gggagatata cagccgttct tcactattgg aaagatgaat
    28801 cgtcttctca acgcgaaaca ctaactgatg cggtccattg tccagttcaa tgctgtccgc
    28861 accgcgcagc agagagctgg aaaccttttt gccgtcgagt accagaagat cgacatcggt
    28921 tgaaagccgc agcgtggtgg caaaaacgga taccggcaaa cataatgcaa tcaaggtggt
    28981 cacgatgccg gttttcatag gtagctcctg tcaaaagacc gcccgaataa taatgtgtca
    29041 aaatttttct ttaatcatac ttactaacat atagacatat ttcccgcttt tgctctcatt
    29101 cattcgtatt agctgcatgg ttggcatgtc ggctttgtcg tacactttgt taaacagtct
    29161 ggaggaaata atgaaagaaa ccgatattgc aggcatttta acgtccactc acaccattgc
    29221 gctggtgggg gcaagcgaca aacccgatcg ccccagctat cgcgtgatga aatatctact
    29281 cgaccagggg tatcacgtca ttccggtttc gccaaaagtt gccggcaaaa cgctgctggg
    29341 ccagcagggg tatggcacgc tggcagacgt tccggaaaag gtcgatatgg tcgatgtttt
    29401 ccgtaattca gaagcggcgt ggggcgtggc acaggaagct attgccattg gcgcgaaaac
    29461 cctctggatg caactggggg tgattaatga gcaagcggca gtactggcac gggatgccgg
    29521 gttaaatgtg gtgatggatc gctgcccggc tattgagatc cctcgcctgg gtctggccaa
    29581 ataaaaaatc cccggaaggc aaaaaccttc cggggatttg ttcagggatt agttacgcag
    29641 acgcggggcc tggagttgtt tgcggatggt ctgcgccagc tcatccatgg atggctgttc
    29701 aggatgttca tcctgcaatt cgctacttag ctgggcttca gccaggtagg tatgaaccgg
    29761 taggccgtta tcgtcctcca tcaccacgtg ataccacgga gcggcgcgaa gctcgtcatt
    29821 caccgccagc tcatcaggcg acggttcaga gagcgaataa accgggtcga tatccacgac
    29881 cactccgaga taacctaaca gggaatggcg gacctgctgg ccgataccga atttgctggc
    29941 aatcatagtc acctcccggg aaatctttac ctttgatata agggtaaaat tccacatttc
    30001 aagttacatg acgcgacagg caaacccttt cagatatagc ccttccgggt aggtagcgat
    30061 caccggatga tcggctgcct gacggaactg ctctataaat tgtacatcac gaccggcatc
    30121 aattgcggca tccgcgatga ttttctgaaa taaatcgctg gtcatcagac cggaacagga
    30181 gaaagtcagg agaataccgc cttcattcag caactgaatc gccagcatgt tgatgtcttt
    30241 ataaccacga cacgcgccca tcaactggct tttattctca acaaacttcg gcgggtccat
    30301 cacgataacg tcaaattttt caccgcgatc gcgataagta cgcagcaatt taaagacatc
    30361 atcacggaca aactcagcct tgctcagatc cagtttgttc agctcaacgt tctgccgtgc
    30421 aatatccagc gcttcctggg aggtatcaac gctgacaacc tggctgcaac cgcccatcag
    30481 tgccgatacg gcgaaaccac cggtatagga gaaacagttc agcacacgtt tattttcaac
    30541 gtagcggcgg gtagccaggc ggctgtctcg ctggtccaga tagtagcccg ttttgtgtcc
    30601 gtgctgaata tccaccagca gcttcatgcc gtgttcttca atcggcagca gggcaggcgg
    30661 caactcgccg gtgacgaggc cctgggtcag ctccatccct tcttttttac gtaccgcaac
    30721 atcgctgcga tcgtaaatcg cacattccgg gtacagcgtt tgcagggcac taattaatgc
    30781 cgcgcgctga tattctgcgc cagcactcag cagttgcagc accagaaaat taccgaaacg
    30841 atcgatagta atacccggca ggccatcaga ttctccggcg attaaacgat agctgtcgag
    30901 gccatctttt tgcgccagcc agtcacgcca tttttgtgct tgttgcaaac ggcgggaaaa
    30961 aaaagcaatg tcgatagact cagacgggtc aaacgtccag acgcgcgccc ggatttgcga
    31021 agctggcgaa taagcgccgc gtgctaacca ttttccctga tgatcaacaa tatcgatggt
    31081 ttcaccgagg ctggctttac cttccatgcg ggcaacggcc ccggaaaaga cccacggatg
    31141 gcgacgaagt aatgattttt cgcgcccttt ggctaacact aaacgtacac tcataattta
    31201 cttttctgtc gatgcaaaga aatgggcgtc attgtccgga gttttccatg gatttgcaac
    31261 gcgtccatta aggataagga gagcagcaat gtcgaaagtc tgcataattg cctggattta
    31321 cgggcgagtt cagggcgtag gatttcgcta caccacacag tacgaagcga aaagactggg
    31381 cttaaccggg tacgccaaaa atcttgacga tggcagcgtg gaagtggttg cctgcggtga
    31441 agaagggcag gtagaaaaat taatgcagtg gttaaagagt ggcggcccgc gttctgcgcg
    31501 tgtagaacgg gtgctcagcg agccgcatca tccctcgggg gaattaaccg attttcgtat
    31561 ccgttaaata cattttaccg gttttggcag gccagcaatt ttggtggctt gcttagccgg
    31621 gcctttcggg aacagtcggt ataaatagcg gctgttacct ttttcttcac caaatttatt
    31681 cgccatcgct tttaccagca tacgaatcgc cggagacgta ttaaattcca ggtagaagtc
    31741 acgtacaaaa cgcaccactt cccagtgttc tggcgacagc gaaatccctt cgttctctgc
    31801 aatcaccact gccagcggtt cactccactg gctgctttct ttgagatagc cttcggtatc
    31861 cgtttctatc tctttacctt cgaagatcag cataattatt actaccttaa tcagactggc
    31921 ggcagtgtaa caaaaaacaa agccccgcat aaagcgaggc tatgaaagtg ttagcgggtg
    31981 agattaatcg cggctggcga agcccagaat gctcagcagg ctgacgaaga tgttgtacag
    32041 cgaaacatac aggctaaccg tggcacgaat atagttcgtt tcaccgccat gaatgatgtt
    32101 gctggtttca aacaagatag cgccagagga gatcagaatg aagaccgcgc tgatcgccag
    32161 atgcagagca ggcagttgca ggaagatatt agcaaccata ccaatcagta ccaccacaat
    32221 acccgccatc agcataccgc cgaggaacga catatctttg cgggtggtca gcacatatgc
    32281 agagcagcag aagaacacta acgccgttcc gcccagtgcc atagcgatta cgtcacccat
    32341 tccggcagac agataggtgt tcagaattgg tccgaggata taacccagaa aaccggtaaa
    32401 ggcgaatgcg gagataatcc cggtcggctt attcgccgtt ttataggtca ggaacatcaa
    32461 accatacata cccaccagcg tcagaatcag acccggagat ggcagcatca gcaccgtact
    32521 ggcagttgcg gtaatcgccg aaaaggccag cgtcaggctc agcagaaaat aggtattacg
    32581 cagcacctta tgggtgctaa gcagtgatgt acggtcatgt gaagaactaa caatacgatc
    32641 cattgagtca ctctctatga cagatgtaat taattaagca gcataatgat aatgcgtaag
    32701 ggcacccaga agttttaccc atctttacgc atttgatctg gaacaggttt aacagcggat
    32761 tatcaggtca ttaagcaaat ataacgccct gagaattccg acaggcaaaa gaaaaagggg
    32821 ttagcattta gctaacccct tatcttattt ggcggaagcg cagagattcg aactctggaa
    32881 ccctttcggg tcgccggttt tcaagaccgg tgccttcaac cgctcggcca cacttccgga
    32941 atgacgcgca ctataaacat cccgatgcgg cgtgtaaacc cctaatttgt ttgtttgcct
    33001 gaaaaacagc caaaagtgca ttgatagcgt gaaataacag cagattgatc atttcatcac
    33061 catgaattcc ttctctttta ctcgtttagc aaccggctaa acatccccat cgcccggcta
    33121 aagaaaaata ggtccatttt tatcgctaaa agataaatcc acacagtttg tgttgttttg
    33181 tgcaaaagtt tcactacgct ttattaacaa tactttctgg cgacgtgtgc cagtgcagaa
    33241 ggatgagctt tcgttttcag catctcacgt gaagcgatgg tttgccttgc tacagggacg
    33301 tcgcttgccg accataagcg cccggtgtcc tgccggtgtt gctaggagaa gagacgtgcg
    33361 atatgaataa cgaggaaaca ttttaccagg ccatgcggcg tcagggcgtt acccggcgca
    33421 gctttctcaa atattgtagt ctggctgcca cgtcgctggg attaggcgcg ggaatggcac
    33481 caaagattgc ctgggcgctg gagaacaaac cgcgcattcc ggtggtatgg atccacggtc
    33541 tggaatgtac ctgctgtacc gaatctttta tccgctccgc tcacccactg acgaaggacg
    33601 tcatcctttc cctgatttcc ctctattacg acgataccct gatggctgcc gccggaaccc
    33661 aggcggaaga agtctttgaa gacatcatca cgcaatataa tggcaaatat atcctcgcag
    33721 tagaaggtaa tccgccgctg ggcgagcagg ggatgttctg tatcagcagc ggtcgaccgt
    33781 ttattgagaa actcaaacgt gccgctgccg gagccagcgc gattatcgcc tggggaacct
    33841 gcgcgtcctg gggctgcgtg caggccgcgc gacctaatcc gacacaggca acgcctatcg
    33901 acaaagtcat caccgacaaa cccattatca aagtacctag ctgcccgccg atcccggatg
    33961 tgatgagcgc catcattact tacatggtga cctttgatcg cttgccagat gtcgacagaa
    34021 tgggtcgtcc actgatgttt tatggtcagc gtatccacga taaatgctat cgccgcgctc
    34081 acttcgacgc cggagagttc gtccagagtt gggatgatga cgctgcccgc aaaggttatt
    34141 gcctgtacaa aatgggctgc aaagggccta ccacctataa cgcctgttcc tccacacgct
    34201 ggaatgatgg cgtttctttc ccaatccagt ctggtcacgg ctgcctgggc tgtgcggaaa
    34261 atggtttctg ggatcgcggt tcgttctaca gccgcgtggt cgatattccg caaatgggta
    34321 ctcattccac cgccgatacc gtcggtttaa ccgcgcttgg cgtggtggca gcagctgttg
    34381 gtgtgcacgc agttgccagc gccgttgacc aacgcagacg tcataaccag caacctacag
    34441 aaaccgaaca tcagccaggc aatgaggata aacaggcatg agcactcagt acgaaactca
    34501 gggatacacc atcaataatg ccggacgccg cctggtggtc gacccgatta cgcgcatcga
    34561 aggccacatg cgctgcgaag tgaatattaa cgatcagaat gtgatcacca atgccgtctc
    34621 ctgcggcacc atgtttcgcg ggctggagat catcctgcaa gggcgcgacc cgcgcgatgc
    34681 gtgggcgttc gttgaacgta tctgcggcgt ctgtactggc gtacacgccc tggcttcggt
    34741 ttacgccatc gaagatgcca tcggtattaa agtgccggac aacgccaata tcatccgcaa
    34801 cattatgctg acaacgctct ggtgccacga tcatctggtg cacttctatc agcttgccgg
    34861 gatggactgg atcgacgtgt tagatgcgct gaaagccgac ccgcgaaaaa cctccgaact
    34921 ggcgcaaagt ctctcctctt ggccgaaatc atcccctagc tatttcttcg acgtacaaaa
    34981 tcgcctgaag aaattcgttg aaggcgggca gttggggatc ttccgcaatg gctactgggg
    35041 gcacccgcag tacaaattgc cgctagaagc caacctgatg ggctttgccc actatctcga
    35101 agctctcgat ttccaacgtg aaattgtcaa aattcacgcg gtctttggcg gtaaaaaccc
    35161 gcatccaaac tggattgtcg gcgggatgcc ttgcgccatt aacattgacg aaagcggcgc
    35221 ggtcggggcg gtcaatatgg aacgcctgaa cctggtgcag tcgattatca cccgcacggc
    35281 agacttcatt aacaacgtga tgatccccga cgccttagcc atcggtcagt tcaacaaacc
    35341 gtggagcgaa atcggcacgg gtctttctga taaatgtgtc ctcagctacg gcgcattccc
    35401 ggatattgcc aacgactttg gtgagaaaag tctgctgatg cctggcggcg cggtgattaa
    35461 cggcgacttc aacaatgtgc tgccagtgga tttggttgat ccgcagcagg tgcaggagtt
    35521 tgtcgatcac gcctggtatc gttatcccaa cgatcaggtc gggcgtcatc cattcgatgg
    35581 cattaccgac ccgtggtaca accccggcga tgtcaaaggc agcgatacca acattcagca
    35641 gctgaatgaa caggaacgct actcgtggat caaagcgcca cgctggcgcg gtaacgcgat
    35701 ggaagtgggg ccgctggcac gcacgttaat cgcttatcac aaaggcgatg ctgcgaccgt
    35761 tgagtcggtc gatcgcatga tgtcggcgtt gaacctgccg ctttccggta tccagtcaac
    35821 gttgggccgc aggtaagttg cagtatttct tcgacaagct gatgaccaac ctgaaaaatg
    35881 gcaatctcgc cactgcttcc actgaaaaat gggaacctgc aacctggccg acagagtgcc
    35941 gtggcgtcgg ctttaccgaa gcgccgcgcg gagcgttagg ccactgggcc gccattcgcg
    36001 atggcaagat tgatctctac cagtgcgtgg tgccgaccac ctggaacgcc agcccgcgcg
    36061 atcccaaagg gcagattggc gcttatgaag cggcgctgat gaacaccaaa atggcgatcc
    36121 ccgagcaacc gctggagatc ctgcgtactc tgcacagctt tgatccgtgc ctcgcctgtt
    36181 caactcatgt gctgggcgac gacggtagcg agctgatctc cgtgcaggtg cgttaacagc
    36241 gaaggagaat catcatgcaa cagaaaagcg acaacattgt cagccactat gtctttgaag
    36301 caccagttcg tatctggcac tggctgacgg tgttatgcat ggcggtgttg atggtcaccg
    36361 gttactttat cggcaagccg ctaccttccg tcagtggcga ggcgacgtat ctgttctata
    36421 tgggttacat caggttaatt cacttcagcg ccgggatgat ttttaccgtg gtcttgctga
    36481 tgcggatcta ctgggctttt gttggcaatc gttactcccg cgagctgttt atcgtgccgg
    36541 tatggcgtaa aagctggtgg cagggcgtgt ggtatgaaat ccgctggtat ctgtttctgg
    36601 caaaacgtcc gagtgccgat ataggccata accccatcgc ccaggcggcg atgttcggct
    36661 atttcctgat gtcggtcttt atgatcatca ccggttttgc gctgtacagc gaacacagcc
    36721 agtacgctat ttttgcgccg ttccgttatg tggtggaatt tttctactgg acgggtggca
    36781 actcaatgga cattcacagc tggcatcggc tggggatgtg gctgattggg gcgtttgtga
    36841 tcggtcatgt ctacatggcg ctgcgtgaag acatcatgtc cgacgacacg gtgatctcca
    36901 ccatggtcaa cggctaccgt agccacaaat ttggcaaaat aagtaacaag gagcgttcat
    36961 gagcgagcaa cgcgtggtgg tcatggggct gggcaacctg ctgtgggccg atgaaggctt
    37021 cggcgtgcgg gtggcggaac ggctgtatgc ccattaccac tggcccgagt atgtggagat
    37081 tgtcgatggc ggtactcagg gactgaactt gctggggtat gtcgaaagcg ccagccatct
    37141 gttgattctc gatgccattg actacgggct ggaacctgga aggctgcgaa cctatgccgg
    37201 agaacgcatt ccggcctatc tcagcgcgaa gaaaatgagc ctgcatcaga acagtttctc
    37261 cgaagtgctg gcgctggcag atatccgcgg acatctgcca gcacatattg ccctcgtcgg
    37321 gctgcaaccc gcgatgctcg acgactacgg cggcagcctg agcgaactgg cacgggagca
    37381 actgcccgct gcggaacaat cggcgctggc gcagttggct gcgtggggaa gtgtgccgca
    37441 accggctaat gaatcgcgtt gtctcaatta tgactgtctg tcgatggaaa attacgaagg
    37501 cgttcgcttg cgccagtacc ggatgacaca ggaggagcag ggatgagcaa cgacacgcca
    37561 tttgatgcgt tgtggcaacg aatgctggcg cgcggctgga cgccggtcag tgaatcccgt
    37621 cttgacgact ggcttacgca agcgccagac ggcgtggtgt tattaagcag tgacccgaaa
    37681 cgcacgccag aggtcagcga taatccggta atgattggcg aattactgca cgagtttccc
    37741 gactatacat ggcaggtggc gattgctgac cttgagcaga gcgaagccat cggcgatcgc
    37801 tttggcgcct ttcgctttcc tgccacttta gtgtttaccg gcggaaacta tcgcggcgtg
    37861 ctgaatggta ttcacccgtg ggcggaactg ataaacctga tgcgcgggct tgtcgaaccg
    37921 cagcaggagc gtgcctcatg agcgaaactt ttttccatct gctggggcca ggaacgcaac
    37981 cgaacgatga cagtttcagc atgaatccac tgccgatcac ctgtcaggtg aatgatgaac
    38041 cgagtatggc ggccttggag caatgtgctc acagcccgca ggtgattgcg ctgttaaacg
    38101 agttacaaca tcaactaagc gaacgccagc cgccgttggg cgaggtgctg gcagtcgatc
    38161 tgttaaatct caacgccgac gatcgtcact ttatcaatac gcttctcggg gaaggggaag
    38221 tgtcagtgcg cattcagcag gctgacgaca gtaaaagtga aatacaggag gcgatcttct
    38281 gcggattatg gcgggtgcgc agacgtcgcg gcgaaaagtt gctggaggac aaactggagg
    38341 ctggctgcgc gccgctggcg ttgtggcagg cggcaacgca aaatctcttg ccgacagatt
    38401 cgctgttacc gccgcccatt gatggcctga tgaatggcct accgttggcg catgagttac
    38461 tggcacatgt acgtaacccc gacgcgcagc cgcacagcat taatctgacg caattaccca
    38521 tcagcgaggc tgatcggctt tttctctcac gtctctgtgg gccgggaaat attcagattc
    38581 gtaccattgg ctatggcgag agctatatca actccacggg gttacgccat gtctggcatt
    38641 tacgctgtac ggacacctta aaaggcccgt tactggaaag ttatgaaatc tgcccaatac
    38701 cggaagtggt gctggcagcg ccagaagatt tggtcgactc tgcgcagcgg cttagcgagg
    38761 tatgtcagtg gctggcggaa gctgcaccga cgtaaaaaga cggtaagtat cgctttcagt
    38821 cttatgaata tcgcaatcgg cgaatacctc tggtcgtaga gtttcaggat aaagagggag
    38881 atctaacatt atcgggttat ttttctctct tcgcctacag gagtgcgcat gtgggatgtc
    38941 attgatttat cgcgctggca gtttgctctg accgcgctgt atcacttttt atttgtaccc
    39001 cttaccctgg ggctgatttt tttgctggct atcatggaaa ccatttacgt ggtcaccggc
    39061 aaaacaatct accgcgatat gacgcgcttc tggggtaagc tcttcggtat caattttgct
    39121 cttggcgtgg ctaccggcct gaccatggag tttcagtttg gtactaactg gtcattctat
    39181 tccaactatg tgggcgatat ttttggcgca ccactggcga tggaagcatt aatggccttc
    39241 ttcctcgaat ccacctttgt cgggctgttc ttcttcggct ggcaacggct gaataaatac
    39301 cagcacctgc tggtgacgtg gctggtggcg ttcggttcaa atctctctgc attgtggata
    39361 ttgaatgcca acggttggat gcaatacccg accggtgcgc attttgatat cgacaccctg
    39421 cgtatggaga tgaccagctt cagcgagctg gtctttaacc cggtcagcca ggtgaaattt
    39481 gtgcacaccg tcatggcggg ctacgtgacc ggggccatgt ttattatggc gatcagcgcc
    39541 tggtatttac tgcgcggacg ggagcgcgat gtcgcattac gctcgtttgc catcggttcc
    39601 gtcttcggta ctctggcgat tatcggtacc ctgcaactcg gagacagttc tgcgtatgaa
    39661 gtcgcgcaag tacaaccggt aaaactggcg gcgatggaag gggagtggca aacggaacct
    39721 gctcctgcac cgttccatgt ggttgcctgg ccggaacagg atcaagagcg taacgccttt
    39781 gccatcaaaa ttcccgcgct gctagggatc ctcgccactc actcattaga taaacccgtg
    39841 ccgggtctga agaatttgat ggctgaaacc tacccacgct tgcaacgcgg acgtatggcc
    39901 tggctgttaa tgcaggaaat atctcaaggc aatcgtgagc cgcatgtgtt gcaggcattc
    39961 cgggagctgg aaggcgacct tggttacggc atgttgctct cccgctatgc gccggatatg
    40021 aatcatgtca cagccgcaca gtaccaggcg gcgatgcgtg gcgcgatacc tcaggttgcg
    40081 ccggtattct ggagtttccg catcatggtg ggctgtggtt ccctgctgct actggtgatg
    40141 ctgattgcgc ttgtccagac gctgcgtggc aaaatcgacc agcatcgctg ggtgctgaaa
    40201 atggcgctct ggagtttgcc gttgccgtgg attgcgattg aagccgggtg gtttatgacc
    40261 gagtttggtc gtcagccgtg ggcgatacag gacatcttac cgacatactc cgcgcactcc
    40321 gctttaacca caggacaact ggctttctca ctgatcatga tcgtaggtct ttacaccctg
    40381 ttcttaatcg ccgaagtcta cctgatgcag aaatatgccc gtctggggcc gagcgcgatg
    40441 cagagtgaac aaccgacgca gcaacagggg taaaggagaa aatcatgttt gattatgaaa
    40501 cattgcgctt catctggtgg ctgctgattg gcgtgatcct ggtggtcttt atgatctccg
    40561 acggatttga catggggatc ggctgtttgc tgccgctggt ggcgcgtaat gatgatgaac
    40621 gccggatagt gattaatagc gttggtgcgc actgggaagg caatcaggtc tggttgatcc
    40681 tcgcaggtgg ggcattattt gccgcctggc ccagagtgta tgcagcggcg ttttccggct
    40741 tttatgtggc gatgatcctg gtgctgtgct cgctgttctt ccgtccgctg gcctttgatt
    40801 atcgcggaaa aattgccgat gcacgctggc gtaaaatgtg ggatgccggt ctggtcatcg
    40861 gcagtctggt gccgccggta gtcttcggta tcgccttcgg caacttgttg ctcggcgtgc
    40921 cgtttgcctt cacaccgcaa ttacgcgtgg agtatctcgg cagcttctgg caactgctga
    40981 cgccattccc tttattgtgt ggtttgctca gccttgggat ggtgattttg caaggtggcg
    41041 tctggttaca actgaaaact gttggtgtga ttcatctgcg ttcacagctg gcgactaaac
    41101 gcgctgcact gttggtgatg ctgtgctttt tgctggcagg ttactggctg tgggtcggta
    41161 ttgatggctt tgtactgctc gcccaggatg ctaacggtcc ttccaatccg ttaatgaaac
    41221 tggtggcagt gctacctggt gcctggatga ataatttcgt cgaatcgccc gttttgtgga
    41281 tcttcccgct gctggggttc ttctgcccat tgctgacggt gatggcgatt tatcgtggtc
    41341 gcccgggttg gggatttttg atggcatcat tgatgcaatt tggcgtgatt ttcacggcag
    41401 gcatcacgct gttccccttt gtcatgccgt caagtgtgag tccaatctcc agcctgacgt
    41461 tgtgggacag cacttccagt cagctgacgc tgagcattat gttggtaatc gtgctgatat
    41521 ttttgcccat tgtgttgctc tacactctct ggagctacta caaaatgtgg ggacgcatga
    41581 caacagaaac tctccgccgt aacgaaaacg agttgtacta aggagcagga acatgtggta
    41641 tttactttgg ttcgtcggca ttttgttgat gtgttcgctc tccacccttg tgttggtatg
    41701 gctggacctg cgtctgaaaa gttaacgaac gtaggcctga tgcggcgcat tagcatcgca
    41761 tcaggcaatc aataatgtta gatatgaaaa gcggaaacat atcgatgaaa gcgatcttaa
    41821 tcccattttt atctcttctg attccgttaa ccccgcaatc tgcattcgct cagagtgagc
    41881 cggagctgaa gctggaaagt atggtgattg tcagtcgtca tggtgtgcgt gctccaacca
    41941 aggccacgca actgatgcag gatgtcaccc cagacgcatg gccaacttgg ccggtaaaac
    42001 tgggttggct gacaccgcgc ggtggtgagc taatcgccta tctcggacat taccaacgcc
    42061 agcgtctggt agccgacgga ttgctggcga aaaagggctg cccgcagtct gctcaggtcg
    42121 cgattattgc tgatgtcgac gagcgtaccc gtaaaacagg cgaagccttc gccgccgggc
    42181 tggcacctga ctgtgcaata accgtacata cccaggcaga tacgtccagt cccgatccgt
    42241 tatttaatcc tctaaaaact ggcgtttgcc aactggataa cgcgaacgtg actgacgcga
    42301 tcctttgcag ggcaggaggg tcaattgctg actttaccgg gcatcggcaa acggcgtttc
    42361 gcgaactgga acgggtgctt aatttcccgc aatcaaactt gtgccttaac cgtgagaaac
    42421 aggacgaaag ctgttcatta acgcaggcat taccatcgga actcaaggtg agcgccgaca
    42481 atgtctcatt aaccggtgcg gtaagcctcg catcaatgct gacggagata tttctcctgc
    42541 aacaggcaca aggaatgccg gagccggggt ggggaaggat caccgattca caccagtgga
    42601 acaccttgct aagtttgcat aacgcgcaat tttatttact acaacgcacg ccggaggttg
    42661 cccgcagtcg cgccaccccg ttattggatt tgatcatggc agcgttgacg ccccatccac
    42721 cgcaaaaaca ggcgtatggt gtgacattac ccacttcagt gctgtttatt gccggacacg
    42781 atactaatct ggcaaatctc ggcggcgcac tggagctcaa ctggacgctt ccaggtcagc
    42841 cggataacac gccgccaggt ggtgaactgg tgtttgaacg ctggcgtcgg ctaagcgata
    42901 acagccagtg gattcaggtt tcgctggtct tccagacttt acagcagatg cgtgataaaa
    42961 cgccgctgtc attaaatacg ccgcccggag aggtgaaact gaccctggca ggatgtgaag
    43021 agcgaaatgc gcagggcatg tgttcgttgg caggttttac gcaaatcgtg aatgaagcac
    43081 gcataccggc gtgcagtttg taatgcataa aaaagagcat tcagttacct gaatgctctg
    43141 aggctgatga caaacgaaga actgtctaat gcgtagaccg gaaaaggcgt tcacgccgca
    43201 tccggtcact ttcagtttta ctctttctcg gagtaactat aaccgtaata gttatagccg
    43261 taactgtaag cggtgctggc gcgtttaatc acaccattga ggattgcgcc tttaatatta
    43321 acgccagcct gttccagacg ctgcattgac aagctcacct ctttggcggt gttcaagcca
    43381 aaacgcgcaa ccagcaggct ggtgccgaca gagcgtccca cgaccgcagc atcgctcacc
    43441 gcca
//
LOCUS       DADMGC010000018        41031 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.018, whole genome shotgun sequence.
ACCESSION   DADMGC010000018 DADMGC010000000
VERSION     DADMGC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.018"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..221)
                     /locus_tag="JNZ73_000985"
     CDS             complement(<1..221)
                     /locus_tag="JNZ73_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HAY8731357.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIG"
     gene            complement(261..1106)
                     /gene="glpF"
                     /locus_tag="JNZ73_000986"
                     /pseudo
     CDS             complement(261..1106)
                     /gene="glpF"
                     /locus_tag="JNZ73_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            1532..1777
                     /gene="zapB"
                     /locus_tag="JNZ73_000987"
     CDS             1532..1777
                     /gene="zapB"
                     /locus_tag="JNZ73_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HAY8731358.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            complement(1862..2347)
                     /gene="rraA"
                     /locus_tag="JNZ73_000988"
     CDS             complement(1862..2347)
                     /gene="rraA"
                     /locus_tag="JNZ73_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HAY8731359.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(2440..3366)
                     /gene="menA"
                     /locus_tag="JNZ73_000989"
     CDS             complement(2440..3366)
                     /gene="menA"
                     /locus_tag="JNZ73_000989"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAY8731360.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            complement(3433..4764)
                     /gene="hslU"
                     /locus_tag="JNZ73_000990"
     CDS             complement(3433..4764)
                     /gene="hslU"
                     /locus_tag="JNZ73_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HAY8731361.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            complement(4774..5304)
                     /gene="hslV"
                     /locus_tag="JNZ73_000991"
     CDS             complement(4774..5304)
                     /gene="hslV"
                     /locus_tag="JNZ73_000991"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HAY8731362.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            complement(5397..6356)
                     /gene="ftsN"
                     /locus_tag="JNZ73_000992"
     CDS             complement(5397..6356)
                     /gene="ftsN"
                     /locus_tag="JNZ73_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HAY8731363.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            complement(6448..7473)
                     /gene="cytR"
                     /locus_tag="JNZ73_000993"
     CDS             complement(6448..7473)
                     /gene="cytR"
                     /locus_tag="JNZ73_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HAY8731364.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            complement(7629..9827)
                     /gene="priA"
                     /locus_tag="JNZ73_000994"
     CDS             complement(7629..9827)
                     /gene="priA"
                     /locus_tag="JNZ73_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAY8731365.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            10030..10242
                     /gene="rpmE"
                     /locus_tag="JNZ73_000995"
     CDS             10030..10242
                     /gene="rpmE"
                     /locus_tag="JNZ73_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAY8731366.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            complement(10303..10911)
                     /locus_tag="JNZ73_000996"
     CDS             complement(10303..10911)
                     /locus_tag="JNZ73_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HAY8731367.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(11095..11412)
                     /gene="metJ"
                     /locus_tag="JNZ73_000997"
     CDS             complement(11095..11412)
                     /gene="metJ"
                     /locus_tag="JNZ73_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HAY8731368.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            11689..12849
                     /gene="metB"
                     /locus_tag="JNZ73_000998"
     CDS             11689..12849
                     /gene="metB"
                     /locus_tag="JNZ73_000998"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAY8731369.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            12852..15284
                     /locus_tag="JNZ73_000999"
     CDS             12852..15284
                     /locus_tag="JNZ73_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HAY8731370.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            15633..16523
                     /gene="metF"
                     /locus_tag="JNZ73_001000"
     CDS             15633..16523
                     /gene="metF"
                     /locus_tag="JNZ73_001000"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HAY8731371.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGICHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            16852..19032
                     /gene="katG"
                     /locus_tag="JNZ73_001001"
     CDS             16852..19032
                     /gene="katG"
                     /locus_tag="JNZ73_001001"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAY8731372.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGVNGARLALMPQRDWDVNAAAVR
                     ALLVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            19093..20031
                     /gene="yijE"
                     /locus_tag="JNZ73_001002"
     CDS             19093..20031
                     /gene="yijE"
                     /locus_tag="JNZ73_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HAY8731373.1"
                     /translation="MSTGKLHTGSLMSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVT
                     SYIGAFDFTALRCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWAL
                     VSGGAGKVAILSYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFS
                     SMKSAMLAILSGVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQ
                     REIDWQPTVFWALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWL
                     LGENPGAVEGSGIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(20058..20675)
                     /locus_tag="JNZ73_001003"
     CDS             complement(20058..20675)
                     /locus_tag="JNZ73_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HAY8731374.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRSISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(20950..22042)
                     /gene="gldA"
                     /locus_tag="JNZ73_001004"
                     /pseudo
     CDS             complement(20950..22042)
                     /gene="gldA"
                     /locus_tag="JNZ73_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            complement(22053..22715)
                     /gene="fsa"
                     /locus_tag="JNZ73_001005"
     CDS             complement(22053..22715)
                     /gene="fsa"
                     /locus_tag="JNZ73_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY8731375.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAEGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPCQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(22727..25228)
                     /gene="ptsP"
                     /locus_tag="JNZ73_001006"
     CDS             complement(22727..25228)
                     /gene="ptsP"
                     /locus_tag="JNZ73_001006"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY8731376.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRVRTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            25537..26616
                     /locus_tag="JNZ73_001007"
     CDS             25537..26616
                     /locus_tag="JNZ73_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HAY8731377.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            26631..26951
                     /locus_tag="JNZ73_001008"
     CDS             26631..26951
                     /locus_tag="JNZ73_001008"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY8731378.1"
                     /translation="MTKIIAVTACPSGVAHTYMVAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            27002..29299
                     /locus_tag="JNZ73_001009"
     CDS             27002..29299
                     /locus_tag="JNZ73_001009"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY8731379.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            29265..30143
                     /locus_tag="JNZ73_001010"
     CDS             29265..30143
                     /locus_tag="JNZ73_001010"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HAY8731380.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            30145..30486
                     /locus_tag="JNZ73_001011"
     CDS             30145..30486
                     /locus_tag="JNZ73_001011"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY8731381.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(30473..31324)
                     /locus_tag="JNZ73_001012"
     CDS             complement(30473..31324)
                     /locus_tag="JNZ73_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8731382.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLRGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(31473..33206)
                     /gene="cptA"
                     /locus_tag="JNZ73_001013"
     CDS             complement(31473..33206)
                     /gene="cptA"
                     /locus_tag="JNZ73_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HAY8731383.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(33389..36040)
                     /gene="ppc"
                     /locus_tag="JNZ73_001014"
     CDS             complement(33389..36040)
                     /gene="ppc"
                     /locus_tag="JNZ73_001014"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HAY8731384.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLHQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLDYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(36392..37543)
                     /gene="argE"
                     /locus_tag="JNZ73_001015"
     CDS             complement(36392..37543)
                     /gene="argE"
                     /locus_tag="JNZ73_001015"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAY8731385.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            37697..38701
                     /gene="argC"
                     /locus_tag="JNZ73_001016"
     CDS             37697..38701
                     /gene="argC"
                     /locus_tag="JNZ73_001016"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAY8731386.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            38709..39485
                     /gene="argB"
                     /locus_tag="JNZ73_001017"
     CDS             38709..39485
                     /gene="argB"
                     /locus_tag="JNZ73_001017"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAY8731387.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            39546..40919
                     /gene="argH"
                     /locus_tag="JNZ73_001018"
     CDS             39546..40919
                     /gene="argH"
                     /locus_tag="JNZ73_001018"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAY8731388.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
BASE COUNT         9343 a        10531 c        11257 g         9900 t
ORIGIN      
        1 ataccgatag ctgcaatttg atcggaactg atatcggctt tcgccagcac ttctaccagc
       61 gtggagcttt gggtggccca gatttccatt gggtcgtgtt ctacccaacc tggttttggg
      121 tagatttgct caaattcgcg ctgcgacacg ctaatgatat tggcatcgtg atccattacg
      181 accgcgcggg agctggtggt gccctggtcg agcgcaacga tatatttttt tcagtcatgt
      241 ttaattgtcc cgtagtcata ttacagcgaa gctttttgtt ctgaaggagt tgtggtttcc
      301 ttttcttcca caacacagat atcgcaaggc aaatggcgac caatcagttt gcggtaggca
      361 aatgcaccta caatcgcgcc aacgataggg ccgaaaagcg gcaccaggaa gtaaggaatg
      421 tctctgccgc cggtaaaggc gacattgccc cagcccgcca gctaggcaaa gactttcgga
      481 ccgaagtcac gcgctgggtt catggcaaaa cctgtcaatg ggcccataga tgcgccaatg
      541 accgcaatca gtagaccaat cagcaaggga gccaaagggc cgcgtggtac accgttgcca
      601 tcgtccgtta acgccaggat aagccccatc agaatagcgg taatcaccat ctcaactgcg
      661 aaagcctgca caaaattgat atgaggatta gggtaagtag agaaagtgcc agccagatca
      721 acactttcaa cgctgccgcg aacaatgtga tgagtctgct cgaagtcgaa aaataaattg
      781 tagtaaagcc cgtaaactaa agccgcagcg cagaaagcgc cggcaacttg tgaaacgata
      841 aaaggaataa ctttgcgctt gtcgaaacag gcaaacagcc acaatgcaat ggtaacagcg
      901 ggattaagat gcgcgccgga aacccctgcg gtcaggtaga tggccattgc cacccccagt
      961 ccccaaatga cactgatttc ccactgacca aaagacgcac cagcgacttt tagtgctgca
     1021 acgcaaccca caccgaaaaa aatcaacaac ccggtaccga ggaattcagc aatgcactgg
     1081 cctttcaagg ttgatgtttg actcataatc ggatcctgaa gagttaatgt ttgttgtatg
     1141 tgtgaaagtc acggacctcc acgatgcttg taggcatgct gttaacttat cgttaacgag
     1201 caaaaacgag aaatatcgaa cttaaaatgt gtgtgcttcg tcataaaatg agcgttatcg
     1261 cgccatttat tccatctgat tgtacatgat gcatgtttgt tagatcattc gcgcaacaaa
     1321 tttattaaca ttttcgattg atgtggatta tgtcactagg agaaggatga aaagtgtggc
     1381 aaaccgtaat ctgcaaggcg tgccgctgga cagcgatggc gcggcttcat acaatcggag
     1441 ctaactaaag tgcgctcgta tttattaagg cgtcaccggt aatcgggacg aggatttttt
     1501 atccatcaac gccttgcaat tcaggagagg tatgacaatg tcattagaag tgtttgagaa
     1561 actggaagca aaagtacagc aggcgattga taccatcact ctgttgcaga tggaaatcga
     1621 agagctgaaa gaaaaaaaca actcactgtc gcaggaagtt caaaatgccc agcatcagcg
     1681 cgaagagctg gagcgtgaga acaaccatct gaaagaacag cagaacggct ggcaggaacg
     1741 tctgcaggcc ctgctgggtc gcatggaaga ggtctgatta cgcttcatct ttcgcgtaat
     1801 ttctttactg tgtaggccaa caggtaacgc agaaaaaagg caccttgcgg tgcctttctt
     1861 atcattcaat atccagcgga tcttctgaaa gaataatccc ggtattgtcg gcataaagat
     1921 ggtcgccgga gaagaaggtg acaccgccaa aattgacgcg gacatcgctt tcgccaatgc
     1981 cttcgccagc ggcaccaacc ggaattgccg ccatcgcctg gatgccgata tccaactctt
     2041 ccaggtcatc tacctgacgc accgcgccgt aaatgaccag accttcccat tcattttgta
     2101 ctgccagacg cgccagttca gcatcgacca gtgcgcgacg aacagaacca ccgccatcga
     2161 cgacaagaac acgaccacgg ccattctgtt cgagcagatc gtacagcaac ccgttgtcct
     2221 cgaaacattt taccgtgatt atttgtccgc caaacgacgc ccgtccgcca aagttggaga
     2281 acagcggttc cacgacgtta acatcttctt gatagatgtc acaaagctcg gaagtatcgt
     2341 atttcatagg cttaacattc agttgctgcg agaattttca gtatatcgcg ctatgtgggc
     2401 tgttggcaaa gtcatcaatt gttaattgat atttgtcagt tatgctgccc actggcttag
     2461 gaatatccct aaaacaaaca gcaggttagt cagtaacgct cccttgacag tacgttccag
     2521 cattggtcgc atcgccaccg ggtccatttc ccgcatcaca taacgggctt gcttcaccag
     2581 taatggtgcc gccagcagga acagccagcc ccacaggcta tgcagcgaaa agagattaaa
     2641 cagcgccaga cacaccagcg agcccatcag caggcaggca tgataacgac gcgcgttcac
     2701 ttcacctaag cgcaccacca gcgtgttttt gccattttcg cggtcgctat tgatatcacg
     2761 caggttatta atattcagta ctgccgttgc cagcaggccg catgcggtcg ccggaaggat
     2821 cagtgccgga atcaatgtat gagcctgtaa ataccagctc cccatgacac tcaaccagcc
     2881 aaagaaaacc agtacggaaa tatcacccag accgatataa ccataaggac gattgcccac
     2941 ggtgtaggtg atagcggcaa tgatcgacaa cccgccaaga atcaggaaac cgacaaaatc
     3001 ggccagcgta tggcatgcca ctgcaaccag tgccagcccg gagagacaga tgagcacgac
     3061 ggtaataatg agcgcccgtt tcatcttttg ctgggtaatg accccttttt gcatgccgcg
     3121 tagcggccca atgcggtcag gtttatcgct gccttttacc gcatcgccgt aatcattggc
     3181 gaggttagaa aggatctgta atagcccggc ggtaattagt gccagcaggg cgaccagcgg
     3241 atcgaagtga ccttgccacc atgccagcgc cgtcccgacg ataattgcag caaaggcgag
     3301 ggggagggtt ttaggtcgta aactttccag ccacgcctga gttcggctaa tttgttgttc
     3361 agtcataata cgcgccaata aaaatggggc ctttcagccc catcaaacaa tgatgaaaat
     3421 gattgaacgc gattatagga taaaacggct cagatcttca tctgccacca acgcatccag
     3481 atgtttgctc acataatctg cgtcaatagt gatattttga ccgcttaaat cgctggcgtc
     3541 gtaggaaatc tcttccatta aacgctccag aacagtgtgt aaacgacgag caccgatgtt
     3601 ttcggtagat tcgttcacct gccatgccgc ttccgcgatg cgtttaatac cggagtcggt
     3661 aaactcgata tttacgcctt cagtcgccat cagtgctttg tactgcacgg tgatagaggc
     3721 attcggctcg gtcagaatac gctcgaagtc gctggtggtc agtgcctgca gttcaacgcg
     3781 gattggcagc cgaccttgca gttccgggat caggtcagac ggtttcgcaa tctggaacgc
     3841 gccagaagcg ataaacagaa tgtggtcagt tttgaccatc ccgtgtttgg tggaaacggt
     3901 gcaaccttct accagcggca gcaggtcacg ctgaacgcct tcacgagaaa catccggacc
     3961 ggaagactcg ccgcgcttac agattttgtc gatttcgtcg ataaacacga tcccgtgctg
     4021 ctcaacagcg tcgatagcgt cttgcttcag ctcttccggg ttcaccagtt tcgccgcttc
     4081 ttcttcaatc agcagcttca tggcgtcttt gattttcagc ttacgcgctt tttgcttctg
     4141 gccgcccagg ttctggaaca tggactgcag ctggctggtc atctcttcca tgcccggagg
     4201 agccataatt tcaacgccca tcggtgctgc ggcaagatcg atctcgattt ctttgtcatc
     4261 aagctggcct tcacgcagtt ttttgcggaa tgcctgacga gcagcggaag gttcctgctg
     4321 ctgttcagtc tgtccccagt tgtttttagc aggtgggatc agcacgtcga gaatacgttc
     4381 ttctgccagt tcttcagcgc gataacggtt tttctcgata gcctggacgc gtaccatttt
     4441 cacggcggca tcggtcagat cgcgaataat agaatcaact tccttaccga cgtagcccac
     4501 ttcggtaaat ttggtcgctt caactttgat gaacggcgca ttcgccagct tagccagacg
     4561 acgggcgatt tcagttttac cgacaccggt cgggccgatc atcaggatat ttttcggggt
     4621 cacttcatgg cgcagctctt cgttgagctg catgcgacgc cagcggttac gcagagcaat
     4681 cgccacagaa cgcttggcgt tgtcctggcc gatgatgtgc ttatccagtt cgctgacgat
     4741 ttcgcgtggg gtcatttcag acatgggaga tccttacgct ttgtagctta attcttcgat
     4801 ggtgtggaaa tggttggtat agatgcaaat gtcgcctgca atatccaacg ccttttcagc
     4861 aatttcacgg gcgctaagtt cagtgttttc taacagcgcg cgcgccgcag cctgggcgta
     4921 agggccgccg gagccgatag caataagatc gttttctggc tgcaccacgt caccgttacc
     4981 ggtgatgata agcgatgcag tttcatccgc gactgccagc agtgcttcaa gtttgcgcag
     5041 catgcgatcg gtacgccagt cttttgccag ctcaacggcg gctttaacca gatggccctg
     5101 atgcatttcc agtttacgtt caaacagttc gaacagcgta aaagcatccg cagtaccgcc
     5161 cgcaaagccc gcgatgactt tgtcgttgta cagacggcgg acctttttca cgttgccttt
     5221 cattacggta ttgcccaacg tggcctgacc atcaccagcg atgaccacat ggccgttacg
     5281 gcgtacgctt actatagttg tcacgagctg accccttggt tacgaataca gagtacaaac
     5341 cccgtacaaa agtacggggc ataatgcaat tatagatggg ggggattttg agggtttcaa
     5401 cccccggcgg cgagccgaat gcagtttgta tgacccgcca tcttcaaccg attgagggtg
     5461 ctgtctgcgt tctctttgcc tttcaccggg ccaatgacca cacgattcca gccattgttg
     5521 gtggtgattt tcgagtcaaa tccttcgaac gccagctgag cacgtaccgt ctctgcctgt
     5581 tccgcgcctc tgaacgaacc gcactgcacc atccagcggc gttcgtcttt tttctccgcc
     5641 gtcggttttg gcgcgtcagc ggcacgagca accggcgcag cttgctgcgg cttcgactgc
     5701 gcggtcgtgt gcgcaggggt ttgcagcaga tcctggtacg gctgctgagt agaagctggt
     5761 ttagactggc gcggttgcgc ctgtaccggc gcggcttgcg acgtacgcgt ctgctgctgc
     5821 cagctttgtt cagtcgtacg cgactgctga gccaggcgtt gctgctccgc taattgctgc
     5881 gcctgacgct ggcgttgtag cgtttgctga cgctgttccg gcgtctgttc attccacgga
     5941 acctcaacca gctgcgttgg ctgctggcgc atatcagcct gcatttgttc aagaagctga
     6001 cgttgttctg gtgtcagttg ctccggcgtt ttcacttcac caccggcaga aggttctgtg
     6061 ggcgcacgca cacccggctg gcgactttcc agctctttaa tgtagcgcca gcgttcttct
     6121 ggttttggtg gtagtccgtt tccggtcact ttctggcttt gcagcgtctc ggactcttct
     6181 ttcttgtgat gcgtaatgaa gtacagacca ccgataaagg tcacaagaac ggcggcagca
     6241 atagcgacca tagcgggaga aaccgcaggc agatttcgtt gctttttccg tgaggtgctc
     6301 ttttttcgcc gcgaaggtgc cggttggctg cggcgtacat aatctcgttg tgccactatc
     6361 gtttcgctgt atttattcgt tcgtcagccc gccatgttac ttaagcggcg ggcctttgac
     6421 cagtcacggc agtcttaaag gtttacttta aggtaacgcg cgtgttgatc cccggatgat
     6481 aagttcgcag tccattaaac gagagccact gccaacgtgt tgcccctgca tttgatcaag
     6541 caataacagc atagcttccc gaccgatttc gtaacgcggc tgcgcgatgg ttgtcagcgg
     6601 cggatcacaa aattgcgtca ggtcgatgtt atcaaaaccg attatggaaa ggtcttccgg
     6661 gactttcagc ccctggcgtt ttgcctgaga aagtgcgccg agcgccatca catcgctatg
     6721 gcagaagact gcagtaggcg gttgtggaag atcaagcagc tgttgcattg ctttgcttcc
     6781 ggcttcgaag gtgaagtcgc cacgggcgat gtattgtgga tcaaccataa ttccgcagcg
     6841 acgcagcgcc tgaacatagc cttgcaggcg gtagtgacac agtggcatct cttcgggacc
     6901 ggctatacag ccaatccgtt tatgcccttg ctcatataaa taatttactg catcaaatgc
     6961 ggcggtcaga ttgtcgatat gaacggtagg cagctccagt tccggtgcaa attcgttcgc
     7021 catcaccatc ggcggcagat tacgctgttc ctcaatgctg gcatcaaacg gcagccttga
     7081 acccagcagc aacatgccat caatttgctt ggtgatgatc aaatcgataa aggttttttc
     7141 ctgctgattt tgatgcgcac agtcgccaat cagcaccaga tatccgtgat ttgccgccgt
     7201 aacttcgata ccgcgaataa tttcgctaaa gaagggatcg cagatatccg ggacaatcac
     7261 cagaatggtg cgggattcat tacgcttgac gttgcgcccc ataggctgcg gtaaataacc
     7321 cacttcccgg gccgcttttt caacccgatt acgggtggcc tgggagactt tatcgggatt
     7381 cattaatgct cgggagacag tcgctgtaga gacttttgcc ttgagggcaa cgtctttcat
     7441 ggtcgcggca gtttcctgct tcttcgcttt cacactcact cctcgcctgg cacgtcaggc
     7501 gtactacatc catgtttact tcacatcggc aacattttta gcagatagcg cgtgaaaacg
     7561 gttacagaat tttcatgaaa agtgtgatga atattgaatt tttcgatccg cctcgcatcg
     7621 taagcggttt aaccctcaat cggatcaaca tccagtaccc atttcacctt gcgggaatcc
     7681 ggtattgtat tgatgagcgc cagcgtacca ctaatgatgt gttgcaagcg cacgcgggaa
     7741 gggtgctgca acaatatctg ccagcgccag cggccgccac gtttaggtgc cagtgccgga
     7801 accggaccga gaacccacag tttgtcgtct gccagcggac tggagaggat cagattacgc
     7861 agttgttgca ggaacagcgg cgcatgctga ttgttatgat cttccgcacg cacaatcaca
     7921 tggctggtcc atggcggcag ttgcatcatc cgtcgctcag ccagcgcctg ttcggcaaag
     7981 gcgtcgtagc ctttatagag caacgtttgc aacagaggat gttccggatg gtgcgtttgc
     8041 agcaccactt cgccttgttt acccgcacgc ccggcacgac cagcgacctg ggtgtaaagc
     8101 tgagcgaaac gctctgccga gcgaaaatcg gcagaaaaca gcgcgccgtc cacatccagt
     8161 aacgccacca gcgtcacatc cggaaagtgg tgacctttcg ccagcatttg tgtgccgatc
     8221 aaaatcctcg caccgccgcg atgtacttcc gccagttgct gttccagcgc ccctttgcgg
     8281 ctggtggtat cgcggtcgat acgagaaatg ggcacatcag ggaacagcgg cgcaagcgtc
     8341 tgttcaagct gttcggtgcc cagccccacg ggtaccaggt gcgtggaacc gcaggaaggg
     8401 cactggcgcg gcaccggacg ctgactgtca cagtggtgac agcgcaaatg ctgttgcgcc
     8461 tgatgcagcg tgtagtagtg atcgcaacgt gggcattcgg caatccagcc gcagtcgtgg
     8521 cacagtagcg caggcgcaaa gccacggcgg ttaagaaaga ggataacctg attgttggcc
     8581 tgtaaatgct ggcgcatacg ggtgattaac gccggagcca gacctgcctg caccttctga
     8641 ccttttaaat ccagcacgtg ttgaattgcc ggacgcgcat tccccgcccg gcgcgtcagg
     8701 cgcagcaggc ggtatttttt ctgctggacg ttgcacaacg tttccagcgc gggcgttgcg
     8761 gagccaagaa taatcgggat ttgctcgcta tgcgcacgat acaccgccag gtcgcgggca
     8821 tgatagcgcc agccttcctg ctgcttgtag gaactgtcgt gctcttcgtc aatgacaatc
     8881 acgccgagat ttttaaacgg cgtaaacagc gcggagcggg tgccgatcac aatcgccgcc
     8941 tcgccatttt tcgctttcag ccacgccgaa agacgctcgc tgtcgttcag accggaatgc
     9001 agaacttcca cgggcgcatt aaaccgttca cgaaaacggg cgatagtttg cggtgtcagg
     9061 ccgatttccg gcaccatcac cagcgcctgt ttgccctgag cgagcacgtt ttccagtaca
     9121 ctgagataaa cctccgtttt accggaaccg gtaacgcccg ccagcagcca ggcagaaaaa
     9181 gtatctgccg cgctatgaat tgcgccaacg gcggtggcct gttcggtatt caatcgcaac
     9241 cgctcaccag aaacggcata gttcgttcgc cagtcgctaa actctggtgt ttcacttgct
     9301 aaatcacaca gacctttttt gcgcagcgcc tgcaacgcgg catcattaaa ttcgagcgtg
     9361 gcaacctggt cgcgccagat tttgccttgc cgtaacgctg ccagcgcctg ttgttgcttg
     9421 ggtgagcgtt tcaggctgtt cagatccacc gcctgacctt gttcagtggc aaaccagtac
     9481 cacatcggcg cgttcgccgc aggccgcccc tggcgtagta aaatcggcaa ggcatgaaac
     9541 agcacatcgc caatcggatg atgatagtaa tctgccgccc atagcagcaa tcgccagacg
     9601 gagtgagtaa acaccggctc accatccagc acttcgacta ccgcttttag ctcagtgagc
     9661 ggcagttcgc tggcatcgct aactgatacc acaatcccga tgcgctcctg ctgtttgcca
     9721 aacggcacgc gcacgcgaca cccagcttta accgtcatgc cttctggcag cagatagtca
     9781 aaggtacgag gaagcggaac gggcaaggca acgtgggcaa cgggcatagc atcatcctga
     9841 cttgaaattc ggtgggttag tatacacatt gccgtagaag agtgcggatc agtttgcata
     9901 cgctggttaa tttctgtatg atttcgcgcc ttcgtacgaa atgatcgtat tgaagctata
     9961 cttttaacat cgcgtggtgt ctggcgttag ggctggaaga gcgacgcggc cttaaaccga
    10021 ggttttccca tgaaaaaaga tattcacccg aaatacgaag aaattactgc tagctgctct
    10081 tgcggtaacg taatgaaaat ccgctccacc gttggtcatg acctgaacct cgacgtgtgc
    10141 agcaagtgcc acccgttctt cactggcaaa cagcgtgatg ttgctaccgg tggccgtgtt
    10201 gaccgcttca acaagcgttt caacatcccg ggcagcaaat aagaccggat ttccgaaaaa
    10261 agcgccgcac ggcgcttttt ttgtgcctga attttggtcg tatcaccacg aaaacagcgg
    10321 ccactctttg gcaaccgtgg tgagttgtgg cgcgtcgaaa acggcttgtg gtgaaactac
    10381 cgtttcctca agcggaatat ttttaccgta gcgttctttg agcttcgcct ggaccagcgg
    10441 gttgctgaga tcaaactctt tcaacttctg ctgttcgccc acgcgcattc ccagcgcgtt
    10501 ctggtaaacc ttccacacca cttccgaaca gtactggcga tcgtcgctcc atgagaagct
    10561 gaaatcatac ggtttaccga gataacgttt tgccgtttgc gtcagttttt gctgttgttc
    10621 aacactcagt ccgccttcaa cgcggcgaac aacgtagttg cccttttcac catgggcaat
    10681 ccactgcttg agcggggtgt atttcaccgg gccgaccgct tcaaaaacgt agggcttttt
    10741 gttacgcatc accagcatac cggtgtggct gtaatcggaa tgggttgcca gttggatcgc
    10801 tttactttgc gatgagcgag agatttgaaa gatgatgtcg ccagtttgcg gctgccaggc
    10861 aaaggcaggt acagaaacca gcaggctgag gatcagcagc ctgtttttca tagttaaacg
    10921 tccatgtata aaaagcggtg ggtcgcagac aacgtgctcg ttgtttatgc cggatgcggc
    10981 gtgaacgcct tatccggcct acaagttcgt gcaaattcaa taaattgcaa tatgacgtag
    11041 gcctgataag cgtagtgcat caggcgattc cactccgcgc cgctcttttc cgctttagta
    11101 ttcccacgtc tccgggttaa tccccatctc acgcatgatc tcttttgccg cttccgggat
    11161 ttcgtcgctg cgctctttac gcagatcggc atcatccggc aaaggttgcc cggtaaaggc
    11221 atgcagaaac gcttcgcaca gcagctcgct gttggtagcg tgacgcaggt tgttcacctg
    11281 acgacgcgtg cgttcatcgg tgaggatttt taacacctta agaggaatgg aaaccgtaat
    11341 ctttttgact tgttcactct tcttgccgtg ttcagcgtat gggctgatat attcgccgct
    11401 ccattcagcc atgagatact taatcctctt cgtcaataaa ttgagaccag accccacaat
    11461 ttatgtgggt actgaccgta aacccgcata gtttaccgta caggcgttac cgtgacatcg
    11521 tgtaatgcac ctgtcggcgt gataatgcat ataattttaa cggctatttg ggatttgctc
    11581 aatctatacg caaagaagtt tagatgtcca gatgtattga cgtccattaa cacaatgttt
    11641 actctggtgc ctgacatttc accgacaaag cccaggaaac ttcatcacat gacgcgtaaa
    11701 caggccacca tcgcagtgcg tagcgggtta aatgacgacg aacagtatgg ttgcgttgtc
    11761 cctcccatcc atctttccag cacctataac tttaccggat ttaatgaacc gcgcgcgcat
    11821 gattactcgc gtcgcggcaa cccaacgcgc gatgtggttc agcgtgcgct ggcagaactg
    11881 gaaggcggtg ctggcgcagt acttactaat accggcatgt ccgcgattca cctggtaacg
    11941 accgtctttt tgaaacctgg cgatctgctg gttgcgccgc acgactgcta cggcggtagc
    12001 tatcgcctgt tcgacagtct ggcgaaacgc ggttgctatc gcgtgttgtt tgttgatcaa
    12061 ggcgatgaac aggcattacg ggcagcgctg gcagaaaaac ccaaactggt actggtagaa
    12121 agcccaagta atccattgtt acgcgtcgtg gatattgcga aaatctgcca tctggcaagg
    12181 gaagtcgggg cggtgagcgt ggtggataac accttcttaa gcccggcatt acaaaatccg
    12241 ctggcattgg gtgccgatct ggtgttgcat tcatgtacga aatatctgaa cggtcactct
    12301 gacgtggtgg ctggcgtggt gattgctaaa gacccggacg ttgtcactga acttgcctgg
    12361 tgggcaaaca atattggcgt gacgggcggc gcgtttgaca gctatctgct gctacgcggg
    12421 ttgcgaacgc tggtgccgcg tatggagctg gcgcagcgca acgcgcaggc gattgtgaaa
    12481 tatctgcaaa cccagccgtt ggtgaaaaaa ctgtatcacc cgtcgctgcc ggaaaatcag
    12541 gggcatgaaa ttgccgcgcg tcagcaaaaa ggctttggcg caatgttgag ttttgaactg
    12601 gatggcgatg agcagacgct gtgtcgtttc ctgggcgggc tgtcgttgtt tacgctggcg
    12661 gaatcattag ggggagtgga aagtttaatc tctcacgccg caaccatgac acatgcaggc
    12721 atggcaccag aagcgcgtgc tgccgccggg atctccgaga cgctgctgcg tatctccacc
    12781 ggtattgaag atggcgaaga tttaattgcc gacctggaaa atggcttccg ggctgcaaac
    12841 aaggggtaaa aatgagtgtg attgcgcagg caggggcgaa aggtcgtcag ctgcataaat
    12901 ttggtggcag tagtctggct gatgtgaagt gttatttgcg tgtcgcgggc attatggcgg
    12961 agttctctca gcctgacgat atgatggtgg tttccgccgc cggtagcacc actaaccagt
    13021 tgattaactg gttgaaacta agccagaccg atcgtctctc tgcgcatcag gttcaacaaa
    13081 cgctgcgtcg ctatcagtgc gatctgatta gcggtctgct acccgctgaa gaagccgata
    13141 gcctcattag cgcttttgtc agcgatcttg agcgcctggc ggcgttgctc gacagtggta
    13201 ttaacgatgc ggtgtatgcg gaagtggtgg gccacggtga agtgtggtcg gcgcgcctga
    13261 tgtctgcggt acttaatcaa caagggctgc cagcggcctg gcttgatgcc cgtgagtttt
    13321 tacgcgctga acgcgctgcg caaccgcagg ttgatgaagg gctttcttac ccgttgctgc
    13381 aacagctact ggtacaacat ccgagcaaac gtctggtggt gaccgggttt atcagccgta
    13441 acaacgccgg tgaaacggtg ttgctggggc gtaacggttc cgactattcc gcgacacaaa
    13501 tcggtgcgct ggcgggtgtt tctcgtgtaa ccatctggag cgacgtcgcc ggggtataca
    13561 gtgccgaccc gcgtaaagtg aaagatgcct gcctgctgcc gttgctgcgt ctggatgagg
    13621 ccagcgaact ggcgcgtctg gcggctcccg ttcttcacgc ccgtacttta cagccagttt
    13681 ctggcagcga aatcgacctg cagctgcgct gtagctacac gccggatcaa ggttccacgc
    13741 gcattgaacg cgtgctggcc tccggtactg gtgcgcgtat tgtcaccagc cacgatgatg
    13801 tctgtttgat tgagtttcag gtgcccgcca gtcaggattt caaactggcg cataaagaga
    13861 tcgaccagat cctgaaacgt gcccaggtgc gtccactggc ggttggcgtg cataacgatc
    13921 gccagttgct gcaattttgc tacacctcag aagtggccga cagtgcgctg aaaatcctcg
    13981 acgaagcggg attacctgga gaactgcgcc tgcgtcaggg gctggcgctg gtggcgatgg
    14041 tcggggcggg tgtcacccgt aacccgctgc attgccaccg cttctggcag caactgaaag
    14101 gccagccggt cgagtttacg tggcagtccg atgacggcat cagtctggtg gcagtactgc
    14161 gcaccggccc gaccgaaagc ctgattcagg ggctgcatca gtccgtcttc cgcgcagaaa
    14221 aacgcatcgg cctggtattg ttcggcaaag gcaatatcgg ctctcgctgg ctggaactgt
    14281 tcgcccgcga gcagagcacg ctttcggcgc gtactggctt tgaatttgtg ctggcaggcg
    14341 tggtggacag ccgccgcagt ctgttgagtt atgacgggct ggacgccagc cgcgcgttag
    14401 ccttcttcaa cgatgaagcg gtcgagcagg atgaagagtc gcttttcctg tggatgcgcg
    14461 cccatccgta tgatgattta gtggtgttgg acgttaccgc cagccagcag cttgctgatc
    14521 agtatctcga tttcgccagc cacggtttcc acgtcatcag tgccaacaaa ctggcgggag
    14581 caagcgacag caataaatat cgccagatcc acgacgcctt cgaaaaaacc gggcgtcact
    14641 ggctatacaa cgccaccgtt ggtgcgggct tgccgatcaa ccataccgtg cgcgatctga
    14701 tcgacagcgg cgataccatt ttgtcgatca gtgggatctt ctccggcacg ctctcctggc
    14761 tgttcctgca attcgacggt agcgtgccgt ttaccgagct ggtggatcag gcgtggcagc
    14821 agggcttaac cgagcctgat ccgcgtgacg acctctccgg caaagacgta atgcgcaagc
    14881 tggtgattct ggcgcgtgaa gcgggttaca acattgaacc ggaccaggta cgtgtggaat
    14941 cgctggtgcc tgctcattgc gaaggtggca gcatcgacca tttctttgaa aatggcgatg
    15001 aacttaacga gcagatggtg caacggctgg aagcggcccg cgaaatgggg ctggtgctgc
    15061 gctacgtggc gcgtttcgat gccaacggca aagcgcgtgt gggcgtggag gcggtgcgtg
    15121 aagaccatcc gctggcatca ctgctgccat gcgataacgt ctttgccatc gaaagccgct
    15181 ggtaccgcga taatccgctg gtgatccgtg ggcctggcgc tgggcgcgac gtcaccgccg
    15241 gggcgattca gtcggatatc aaccggctgg cacagttgtt gtaatttcag aaatttaata
    15301 atgcccggta ctcatgtttt cgggtctatg gtttctaatg aaatatattg aattatcata
    15361 gaattaggcc ggatgaagcg tttacaacga atccggcaag aagcaataag tacatggtta
    15421 gtttatattt gcagtccggt ttgctttgca tatcggattt tctttttctt accatcctga
    15481 agttttttca tcttccctga tttttcctca ccatcattgg tcatttttcg gttgacgccc
    15541 ttcggctttt ccttcatctt tacatctgga cgtctaaatg gatagatgtg cacaacacaa
    15601 catataacta caagcgattg atgaggtaag gtatgagctt ttttcacgcc agccagcggg
    15661 atgccctgaa tcagagcctg gcagaagtcc aggggcagat taacgtttcg ttcgagtttt
    15721 tcccgccgcg taccagtgaa atggagcaga ccctgtggaa ctccatcgat cgccttagca
    15781 gcctgaaacc gaagtttgta tcggtgacct atggcgcgaa ctccggcgag cgcgaccgta
    15841 cgcacagcat tattaaaggc attaaagatc gcactggtct ggaagcggca ccgcatctta
    15901 cttgcattga tgcgacgccc gacgagctgc gcaccattgc acgcgactac tggaataacg
    15961 gtatttgtca tatcgtggca ctgcgtggcg atctgccgcc gggaagtggt aagccagaaa
    16021 tgtatgcttc tgacctggtg acgctgttaa aagaagtggc agatttcgat atatccgtgg
    16081 cggcgtatcc ggaagttcac ccggaagcaa aaagcgctca ggcggatttg cttaatctga
    16141 aacgcaaagt ggatgccgga gccaaccgcg cgattactca gttcttcttc gatgtcgaaa
    16201 gctacctgcg ttttcgtgac cgctgtgtat cggcgggcat tgatgtggaa atcatcccgg
    16261 gcattttgcc ggtatctaac tttaaacagg cgaagaaatt tgccgacatg accaacgtgc
    16321 gtattccggc gtggatggcg caaatgtttg acgggctgga tgacgatgcc gaaacccgca
    16381 aactggtggg cgcgaatatt gccatggata tggtgaagat tttaagccgt gaaggagtga
    16441 aagatttcca cttctatacg cttaaccgcg ctgaaatgag ttacgcgatt tgccatacgc
    16501 tgggtgttcg acctggttta taaatagtgt ggcttttgtg aaaatcacac agtgatcaca
    16561 aattttaaac agagcacaaa atgctgcctc gaaatgaggg cgggaaaata aggttgtcag
    16621 ccttgttttc tccctcatta cttgaaggat atgaagctaa aacccttttt tataaagcat
    16681 ttgtccgaat tcggacataa tcaaaaaagc ttaattacga tcaatttgat ctacatctct
    16741 ttaaccaaca atatgtaaga tctcaactat cgcatccgtg gattaattca attataactt
    16801 ctctctaacg ctgtgtatcg taacggtaac actgtagagg ggagcacatt gatgagcacg
    16861 tcagacgata tccataacac cacagccact ggcaaatgcc cgttccatca gggcggtcac
    16921 gaccagagcg cgggggcggg cacaaccact cgcgactggt ggccaaatca actccgtgtt
    16981 gacctgttaa accaacattc taatcgttct aacccgctgg gtgaggactt tgactaccgc
    17041 aaagaattca gcaaattaga ttactacggc ctgaaaaaag atctgaaagc cctgctgaca
    17101 gaatctcagc cgtggtggcc agccgactgg ggcagctacg ccggtctgtt tattcgtatg
    17161 gcttggcacg gcgcaggtac ttaccgttca atcgatggac gcggcggcgc gggtcgtggt
    17221 cagcaacgtt ttgcaccgct gaactcctgg ccggataacg taagcctcga taaagcgcgt
    17281 cgcctgttgt ggccaatcaa acagaaatat ggtcagaaaa tctcctgggc cgacctgttt
    17341 atcctcgcag gtaacgtggc gctggaaaac tccggcttcc gtaccttcgg ttttggtgcc
    17401 ggtcgtgaag acgtctggga accggatctg gatgttaact ggggtgatga aaaagcctgg
    17461 ctgactcacc gtcatccgga agcgctggcg aaagcaccgc tgggtgcaac cgagatgggc
    17521 ctgatttacg ttaacccgga aggcccggat cacagcggcg aaccgctttc tgcggcagca
    17581 gctatccgcg cgaccttcgg caacatgggc atgaacgacg aagaaaccgt ggcgctgatt
    17641 gcgggtggtc atacgctggg taaaacccac ggtgccggtc cgacatcaaa cgtaggtcct
    17701 gatccagaag ctgcaccgat tgaagaacaa ggtttaggct gggcgagcac ttacggcagc
    17761 ggcgttggcg cagatgccat tacctctggt ctggaagtag tctggaccca gacgccgacc
    17821 cagtggagca actatttctt cgagaatctg ttcaagtatg agtgggtaca gacccgcagt
    17881 ccggctggag caatccagtt cgaagcggta gacgcaccgg aaattatccc ggatccgttc
    17941 gatccgtcga agaaacgtaa accgacaatg ctggtgaccg acctgacgct gcgttttgat
    18001 cctgagttcg agaagatctc tcgtcgtttc ctcaacgatc cgcaggcgtt caacgaagcc
    18061 tttgcccgtg cctggttcaa actgacgcac agggatatgg ggccgaaatc tcgctacatc
    18121 gggccggaag tgccgaaaga agatctgatc tggcaagatc cgctgccgca gccgatctac
    18181 aacccgaccg agcaggacat tatcgatctg aaattcgcga ttgcggattc tggtctgtct
    18241 gttagtgagc tggtatcggt ggcctgggca tctgcttcta ccttccgtgg tggcgacaaa
    18301 cgcggtggtg tcaacggtgc gcgtctggca ttaatgccgc agcgcgactg ggatgtgaac
    18361 gccgcagccg ttcgtgctct gcttgttctg gagaaaatcc agaaagagtc tggtaaagcc
    18421 tcgctggcgg atatcatcgt gctggctggt gtggttggtg ttgagaaagc cgcaagcgcc
    18481 gcaggtttga gcattcatgt accgtttgcg ccgggtcgcg ttgatgcgcg tcaggatcag
    18541 actgacattg agatgtttga gctgctggag ccaattgctg acggtttccg taactatcgc
    18601 gctcgtctgg acgtttccac caccgagtca ctgctgatcg acaaagcaca gcaactgacg
    18661 ctgaccgcgc cggaaatgac tgcgctggtg ggcgggatgc gtgtactggg tgccaacttc
    18721 gatggcagca aaaacggcgt cttcactgac cgcgttggcg tattgagcaa tgacttcttc
    18781 gtgaacttgc tggatatgcg ttacgagtgg aaagcgactg acgaatcgaa agagctgttc
    18841 gaaggacgtg accgcgaaac cggcgaagtg aaatacacag ccagccgtgc ggatctggta
    18901 tttggttcta actctgtcct gcgtgcagta gcggaagttt acgccagcag cgatgcccac
    18961 gagaagtttg ttaaagactt cgtagcggca tgggtgaaag tgatgaacct cgaccgtttc
    19021 gacctgctgt aatctgacct tcttcagcga ctgtctttca ggcagtcgct gaagtttctt
    19081 taccggcgta tagtgtccac aggaaaacta cacactggat ctctcatgtc tgccgcagga
    19141 aagagcaacc cactggcaat cagtggcctg gttgtgctca cacttatctg gagttatagc
    19201 tggattttca tgaagcaagt caccagttac atcggtgcct tcgactttac cgccttacgc
    19261 tgcattttcg gcgctctcgt tttattcatc gtccttttat tacgtggtcg cggaatgcgc
    19321 ccgacgccgt ttaaatacac cttagccatt gccctgttac aaacctgcgg gatggttggt
    19381 ctggcgcagt gggcgttggt cagcggaggt gcggggaagg tggcgatcct gagctatacc
    19441 atgccgttct gggtggtgat tttcgccgcg ttgtttctcg gtgaacgcct gcgacgtggg
    19501 caatatttcg cgattctgat tgccgctttc ggcttatttt tggtgttgca gccgtggcaa
    19561 ctcgatttct cttcgatgaa aagtgccatg ctggcaatcc tctccggtgt tagttggggg
    19621 gcgagcgcga ttgttgctaa acgcctatac gcccgtcatc cgcgcgtgga tttattatcg
    19681 ttaacatcct ggcagatgct gtatgcggcg ctggtgatga gtgtggtcgc tttactggtg
    19741 ccgcaacgtg aaattgactg gcagcccacc gtgttctggg cgctggccta cagtgcgatt
    19801 ctggcgacgg cgctagcgtg gagcttatgg ctgtttgtgc tgaaaaactt gcctgccagt
    19861 attgccagct taagcacact ggccgttccc gtttgtggcg tactcttttc ctggtggctg
    19921 ctcggtgaga atccgggggc cgttgaaggt agcggtattg tgctgattgt gctggcactg
    19981 gcgctggtga gccgtaagaa aaaagaagct gtcagtgtaa aaagcatctg aattttttct
    20041 tcatgtgggg cgatctctta tttaacaaaa taacgataat gccccaccat ccgccagcta
    20101 aacagcacat cttcttcctg cgcgcctgcg ccaatgttat gtatcaccag cggcgtaccg
    20161 tcgcgggcga agccatctga cgccacccca atatgcgcca gcccgttatc taatcgccag
    20221 gagacaatat cgcccgcttg atagtcaccg gcatttttac tgatggagcg tgttttgttg
    20281 tggcggctaa accaggtttc cagattaggc acccgacggt gatcgatgtt gctgtccggg
    20341 cgctttaact tccatttttg cgggtactca gcaaaattct tcgccatatc ttcgtgaacc
    20401 agtttctgca aatcgacctt ctggctgcgc aatgcgcgga tcaccacatc ggaacataca
    20461 ccgcgttctt gcggaacatc accgccagga taggtaagct gcatatacgc cggatcgtaa
    20521 aatagcgtgc tgccaatttg ctgtctggca ccgtctgcga tggcaaggtt ggtattggcc
    20581 tggatttgta ccacggttgg tggaacggcg ggagatttta aggagtggct ggtaaatgcc
    20641 gttagcaggc tgagcagcgc cagtgaagct ttcatctggt tcgtccgtga aaataagagt
    20701 cacagattcg accttccggg ctaacgattg ttctgtggag aaaatgtcca gaagcggata
    20761 aaaaactcct gaacaacgtg ggagtttcag attactggca cagagtgccg gatgcggagt
    20821 gaacgcctta tccggcctac aaaagcacgc aaattcaata tattgcagag attgcgtagg
    20881 cctgataagc gtagcgcatc aggcaatttt gcgttcaaac tcccggacaa gccgggagtt
    20941 tggagtaggt tattcccact cttgcaggaa acgctgaccg tactggtcag ctaccagcag
    21001 tgcggcgtaa acctgatctg gcgtcgcgcc gccaggcatg ttgtggatgg tttcaccttc
    21061 tgcacaagat gcttttgcaa caatgcgcat tttcgccggg acatcttctt taatatccag
    21121 ttgagcgaga gttattggca aacctaccgc atggctaagt gcagctacgg tttcgatttc
    21181 ctccaccggc gcgttttcca gtaccagctg cgtcagcgta ccaaatgcca ctttttcacc
    21241 gtgataatag tgatgcgcgt ccgggatagc ggtcaggccg ttatgcaccg cgtgagccgc
    21301 cgctttcaaa accaacgccg ctcaaatagg tgttcgcttc aatcacgcgc tccagcgccg
    21361 gggttactac atgctgttcg gcggcaagca tcgctttttc gccttcttcc agcagggtgt
    21421 tgtagcacag ttcagccagt gccagcgccg cctgggtgca cttgccgccc gccatggtgg
    21481 tcgcgccgct acgagagcaa gcacgcgctt caaaccaggt tgccagcgca tcgccgatac
    21541 ccgctgctaa cagacgtgca ggtgcgcccg cgacgatttt ggtgtcgaca atgaccatat
    21601 tcgggttatt tggcaacagc agatagcggt caaactcacc ctcatcggtg tagataacag
    21661 acaatgcgct gcacggtgca tcggtagagg cgatagtcgg tgcgatcgct accggaacac
    21721 ccatgaaatg tgccagtgct ttggcagtat cgagggtttt tccgccaccg ataccgagaa
    21781 ttgcgccaca ctgcgcagtc tccgcgatgc cacgcagacg gtcgatctca ttttgcgaac
    21841 attcaccgcc aaacggcgta atttctacta ccagtccagc atctttaaag cttttctcga
    21901 cagtggattg agcaaaacct aaaacaaatt tgtcacccac cactaaccag cgttctgcca
    21961 gcggcttcag gtattcgccc agacgattaa tcacatcagc gccctggatg tatttacccg
    22021 gtgattgaat aatgcggtcc ataattgctc ctttagagat gagtagtgcc aaatgcggca
    22081 ttccagtcgt gttcgaactt ctctatagct gactctactg caggggtgtt gagcatttgt
    22141 tgcgctacat ctaagggcag ggtgatggat tcacatcctg ccagtaaaca gtccagcgcc
    22201 tgacacggcg ttttaaagct ggctgccagc accatgcttt ctggcgcgtg catttctaac
    22261 agcgtttgca gctcctgaac cgtacgaatg ccgtccccgc cctgggcatc tacgcggtta
    22321 acatacggag caacgtattt tgcccctgcc agtgcggcta ataacccttc tgcggcgcta
    22381 tatacagcgg tgccaagtgt agtaataccc tcttttttca gtattttaat tgctgccaga
    22441 ccttcggaag tcaccgggat tttcaccaca atacccggaa tagcgtcgcg caggcgcttc
    22501 gcttcttcca ccatcccctg cgcgtcgcgg ctcatggtct gagcaaacag aatgccctca
    22561 tcaccaatcg ctttttgcag acgcggcagc acttcccaga tggactcctt gctggcagcg
    22621 ataatgctcg ggttagttgt caccccggca atcgggaata tgcgtgccag acgttcgact
    22681 tctgcgacgt tagcggtgtc cagatacagt tccatgatgt ttccttttac agttccagtt
    22741 catgttgcag caggctggcg atagcgtcgg cagaggcggc gttaaccagc gcgttacgga
    22801 attcttcgtg catgatgcgg cgagcgaggc gcgagaaaat gcgcatgtgc tgatcgcccg
    22861 cagcgtgttt gttcagggtt aacataatga tgaattgcgc ttcatcatcg ccccagcgca
    22921 ccggcgcttg cagacgcgcc acgctgatgg tggattgctc aatgtgttct gatttgctgt
    22981 gtggaatggc aaaactaaag cccagaccgg tagagaaaac ggcctcgcgc gcccataagt
    23041 cggcttccag tttacgcgga tagcggcagc ggcccgccag cagcaggtta tcggtcatgc
    23101 ctttgagcac ttcttctttg ctgcgccagt cactttccag cgtgatgcac tcggcggtga
    23161 ccagcggtgc gtcctgttgg gtcatgcgga attgcgccag caggtgttcc acttccagcg
    23221 aagtacggca ggccattgcc tggttgagca acttgcggca ctcacggcta tcaagttgcg
    23281 ccatccgcgc tttcgccgcc ggaattgatg gtgcgctcat gctgagttca tccagcccta
    23341 agccgaccag caacggcagc acggaacctt tcgctcccag ctcaccgcac agaccaatcc
    23401 atttaccctg gcgatgcacc gcctgcacgg cgtaatcgag cgcccgcaag aatgccggat
    23461 tcaggctgtt gtagtgacga gtaaccttag cgttatcgcg atccactgcc agcagatatt
    23521 gcgtcaggtc attactacca atactaaaga aatcaatctc ttcgcagcat tgatcgatga
    23581 tgaacatcac cgacggcact tccagcataa tgccgagctg gatcttctca tcaaacggaa
    23641 tgtgttcgtt acgtagttgc tgtttggctt ctgccagttt ttctttcacc cataagatct
    23701 cttccattga ggagatcatc gggatcatga ttttcaggct gccgtgagcg gaggcgcgca
    23761 ggatcgagcg tagttgtgtg gtaaataacg acgcgtattc ttcataaata cgcacggcgc
    23821 gataaccgag gaacgggttt gcctctgcgg gaatgtttag ataatcaacg ggtttgtcgc
    23881 cgccaatgtc catagtgcgc acaataatgc tgcgtccgtt ggcagactcc agcgcctgac
    23941 aaaaaatgtt gtacagctcg ctttcgcccg gtgcactggt gcgatccata tagagcattt
    24001 cagtgcggaa caaaccaacg ccttccgcac cattgccgaa tgcggcctgc gcttccacgg
    24061 agtgagcgat gttagcggca atttcaatgc ggataccgtc agcggtacgg gcttgttggg
    24121 tcagccagac acgctgttgc tcacgcaggg cgtcctgtac gcgggcttct tgctgataat
    24181 aacgagctac ggcttctcct ggctcaacca caatcgcccc ggcgttgccg tcgatataaa
    24241 tcgtttgatg ctgccacgga gtgagggcat caatatccac accaaccagc gtcggaatgt
    24301 tgaacgaacg ggcaaggatc accgtatgtg aggtggtgcc gccgcttttc agtaacaatc
    24361 ctttgaggtg atttttatcc agttcgagga actggctggg ggtcagttca tcagccatac
    24421 aaatggcgga ctgcgtcagt ttgcccggtg ccgggaagcg ttgctcaccg tagatttgct
    24481 ggagtaactg gaagcagacg tcgcgtacgt ccagggcacg ttcttgcagg tatctgctgc
    24541 tggaacgggc gaactcttcg caaaagtgat tcgcactggc gacaatcgct tcggcgcagc
    24601 ttaatccggc gctgactcct gccagtaaat gttcgtgcag ggaagtatcg ccagccaggg
    24661 atcggtgcgc ttccagaatc gcgctggtag caccgtcgct atccagcaga cgaaactcaa
    24721 tgtttttcag taccagcgtc aggccgtttt ccagtgcgga ttgctcggcg tcaacgtctt
    24781 tggctgcggg aagattactc agcgcattga gatctaaaga agagatcggc gtcagaatgc
    24841 cgcctgcact accgctgcac acggtgcgga cgcggataat ttgcggattc agattggtca
    24901 gtgaaaccgg cagtggttcc agttcgtcag atttaacttc cgccagcggc gcatcgcagt
    24961 gggggaattc atcgcgcagc cattggctta aacgctggtg cccttcctgt tcgtcggccc
    25021 cggaaatcag tagctggcag ttatcaccca ccagcgtatc ggtgccaatc agcgccagag
    25081 cgcttttggc gttgccttta cggtcagtac gcaggttatg ccactcaatt tgagatgaaa
    25141 aagtattaca cagcgtttca acgtggcttg ccggacgcgc atgtacgccg ttaggtagct
    25201 cacaaataaa ttccacaatc agggccattg cctttctccc ggtacggttt tctgtttaca
    25261 ggataagagc gcacggtaac ggcctgccat gtgacaaatc tgccaaaagc tggacaaatg
    25321 taatgtaacc gtcaatttgc gacgcgtctc acaagacgcc gttttgcggc atgcttccgg
    25381 attatcgcaa gttatgaggc ggatcgcatt tttgtactga tattacaaaa gtccagtaaa
    25441 tggccttttt atccactgtt tgctccgcct gcgattgcct attgtttcct ccatcaaaaa
    25501 tatggacatg ggcttttgca agcccgggag cagcttatga atgagttggt gcagatcctg
    25561 aaaaataccc gtcagcattt aatgacgggc gtttcacaca tgattccctt cgtggtatcg
    25621 ggcggtattt tgctggcggt ttccgtcatg ttgtatggca aaggcgcagt gccggatgcc
    25681 gtagccgatc cgaatttgaa aaaactgttt gatattggcg ttgcaggttt gacgctgatg
    25741 gtgcctttcc tcgccgctta cattggttac tccattgcag agcgttctgc gctggctccg
    25801 tgcgctatcg gtgcctgggt tggtaacagc tttggtgcgg gcttctttgg tgcgctgatc
    25861 gccgggatta tcggcggcat cgtggtgcat tacctgaaga aaattccggt gcataaagtt
    25921 ctgcgctcgg tgatgcccat cttcattatt cctatcatcg gcacactgat taccgcaggc
    25981 atcatgatgt ggggcttggg cgagcctgta ggggcgttga ccaacagcct gactcagtgg
    26041 cttcagggga tgcagcaggg cagcattgtt atgctggcgg tgatcatggg gctgatgctg
    26101 gcgttcgata tgggcggtcc ggttaacaaa gtggcctatg ccttcatgct gatttgcgtt
    26161 gctcagggtg tttataccgt ggtggccatt gctgccgtgg gtatttgtat cccgccgctg
    26221 gggatggggc tggcgacgct gattggtcgt aaaaatttct ccgcagaaga gcgcgaaacc
    26281 ggcaaagcgg cactggtgat gggctgtgtt ggcgttactg aaggggcgat tcctttcgcc
    26341 gctgccgatc cgctgcgtgt cattccttcc attatggtcg gttcagtttg tggtgcagta
    26401 actgcggcgc tggtcggtgc gcagtgctat gcaggctggg gtggtctgat tgtgctgccg
    26461 gtggttgaag gtaagttggg ttatatcgca gcagtggctg tcggagcagt ggtgacggct
    26521 gtttgtgtga acgtgctgaa aagtctggcg cgtaaaaatg ggtcttcgac tgatgaaaaa
    26581 gaagacgacc tggatttgga ttttgaaatt aattaattga ggaacgagcc atgacgaaaa
    26641 ttattgcagt aaccgcatgt ccttcaggtg ttgcccatac ctatatggta gcagaggcgc
    26701 tggaaagtgc cgcgaaagcg aaaggctggg aagtgaaagt agaaacccag gggtcaattg
    26761 gtctggaaaa tgaactgact gcggaagacg tggcgagcgc tgatatggtg attctgacca
    26821 aagatatcgg catcaagttt gaagagcgtt ttgctggcaa gaccatcgtg cgcgtcaaca
    26881 tcagcgatgc agtaaaacgt gctgacgcca ttatgagcaa aattgaagct cacctggcgc
    26941 aaaccgctta agttcttctc ccgctcgcaa gggcgggttc gctttctcac aggagttcct
    27001 catgacgaat cgtatctctc gcctcaaaac tgcactgttt gccaataccc gtgaaatctc
    27061 gctggagcgg gcgctgcttt ataccgccag ccatcggcaa accgaaggcg aaccggtgat
    27121 attgcgccgg gcgaaagcaa cagcgtatat ccttgaacat gttgaaattt cgattcgtga
    27181 tgaagaactg attgccggta accgcaccgt aaaaccgcgc gccgggatta tgtcgccgga
    27241 aatggaccct tactggctgc tgaaagagct ggatcaattc ccgacgcgtc cgcaggaccg
    27301 ctttgctatc agcgaagaag ataaacgtat ctaccgtgaa gagttgttcc cgtactggga
    27361 aaaacgttcg atgaaagatt tcatcaacgg gcagatgaca gatgaagtaa aagccgcgac
    27421 caacacgcag attttcagca tcaaccagac ggataaaggc caggggcaca ttattattga
    27481 ttacccacgc ctgttgaatc acggtctggg tgagctggtg gcacagatgc agcaacattg
    27541 tcagcaacag ccggagaatc acttttatca ggccgcgttg ttactgctgg aagcctcgca
    27601 gaaacacatt ttgcgttacg ccgaactggc ggaaacgatg gcggcaaact gcacagatgc
    27661 ccagcgtcgc gaagagctgc tgactattgc ggagatctcc cgccataacg cgcaacataa
    27721 gccgcagacg ttctggcagg cgtgccagtt attctggtac atgaacatca ttctgcaata
    27781 cgaatccaac gccagttcgc tgtctttagg gcgcttcgac cagtatatgt tgccgttcta
    27841 tcaggcatct ttaacccagg gcgaagatcc ggcgttcctg aaagaactgc tcgaatcttt
    27901 atgggtgaaa tgcaacgaca tcgtgctgtt gcgctccacc agtagcgcgc gttatttcgc
    27961 cggtttcccg accggctata ccgcactgct cggcgggtta accgagaacg gacgtagcgc
    28021 ggtgaacgtg ctttcgttcc tttgccttga cgcctatcaa agcgtgcaat taccgcaacc
    28081 gaacctcggc gtgcgcacta acgccttgat cgacacgccg ttcctgatga aaaccgccga
    28141 aaccattcgc ctcggcaccg gtattccgca aatctttaac gatgaagtgg tggtgccagc
    28201 gttcctcaac cgtggcgttt cgctggaaga tgcgcgcgac tattccgtag tgggctgtgt
    28261 ggaattatct attcccggca gaacctacgg cttgcatgac atcgcgatgt ttaatctgct
    28321 gaaagtgatg gaaatctgcc tgcatgaaaa tgaaggcaat gctgcgctga cttatgaagg
    28381 tttactggag cagatccgcg ccaagatcag ccactacatc accctgatgg ttgagggcag
    28441 caatatttgt gatatcggcc atcgcgactg ggcacctgta ccgctgctct cgtcttttat
    28501 cagcgattgt ctggaaaaag gtcgcgatat taccgatggc ggcgcgcgtt ataacttctc
    28561 cggcgtacag ggaattggta tcgccaacct gagcgattct ctccatgcgt tgaaagggat
    28621 ggtttttgag caacagcgtt taagttttga cgaattgctg tcggtattaa aagccaactt
    28681 tgcaacgcca gaaggcgaaa aagtccgcgc tcgcttaatt aaccgctttg agaaatacgg
    28741 taacgatatc gacgaggtgg ataacattag cgccgaactg ttgcgccact actgcaaaga
    28801 agtggaaaaa taccagaacc cgcgcggcgg ttacttcacg ccgggatcgt ataccgtttc
    28861 tgctcacgtt ccgttgggat cggtggttgg cgcgacgcca gacggtcgtt ttgccgggga
    28921 acaactggca gacggcggct tgtcacctat gctgggtcag gacgcacaag ggccaacggc
    28981 ggtactgaag tcagtcagta agctcgataa tacgctgctg tctaacggta cgttgctgaa
    29041 cgtgaaattc actccggcga ccctggaagg tgaagcggga ttacgcaaac tggccgactt
    29101 cttacgggtg tttacccagc ttaagttgca gcatattcag tttaacgtgg tgaacgccga
    29161 cacgttgcgg gaagcgcaac agcgcccaca agattatgcc gggctggtgg tgcgcgttgc
    29221 tggatacagc gccttctttg tcgaactgtc gaaggagatc caggatgaca tcatccgccg
    29281 gacagcgcat cagctgtaac gttgtggaaa cgcgccgcga tgatgtggcg cgcattttca
    29341 acattcagcg ttattcactg aatgacggcg agggcattcg tacggtggtc ttttttaaag
    29401 gctgtccgca tctttgcccg tggtgtgcta atccggagtc gatctccggc aaaatccaga
    29461 cggtacgcag agaggcgaaa tgtctgcact gtgcgaaatg tttgcgtgat gcggatgaat
    29521 gcccctccgg ggcgtttgaa cggattggtc gcgatatcag ccttgacgct ctggaacggg
    29581 aagtgatgaa agatgacatt ttctttcgca cgtccggcgg cggcgtcacg ctttctggcg
    29641 gcgaagtgtt aatgcaggcg gagtttgcta cccgtttttt acagcgactg cggctgtggg
    29701 gtgtctcatg cgccattgaa actgccggag acgcgccagc aagcaagctg ttaccgctgg
    29761 cgaaattgtg cgatgaagtg ctgttcgatt taaaaattat ggacgcgact caggcgcggg
    29821 atgtggtgaa gatgaacctg ccacgcgtgc tggagaatct gcgtttgctg gtgagtgagg
    29881 gtgtcaacgt gatcccgcgt ttaccgttga tccctggttt cacgctcagc cgggagaata
    29941 tgcagcaggc gctggatgta ctgatcccgc tgaaaatcag gcagatccat ctgttaccgt
    30001 ttcatcagta cggcgaaccg aaataccgcc tgctggggaa aacatggtcg atgaaagagg
    30061 tgcctgcgcc gtcgtcagcc gatgtgacaa cgatgcgcga aatggcagaa cgggccggat
    30121 ttcaggttac cgtgggaggt taaaatggca tacctggtgg cagtaaccgc ctgcgtaagc
    30181 ggcgtggcgc atacttatat ggcggcggaa cggctggaaa agttgtgcca gttagagaag
    30241 tggggcgtca gcattgaaac tcagggcgcg ctgggaacgg agaatcgttt agcggacgag
    30301 gatatccgtc gggcggatgt tgctctgttg attacggata tcgagcttgc cggtgccgag
    30361 cgatttgaac attgccgcta tgtgcaatgc agcatctacg cattcctgcg tgagccgcag
    30421 cgggtaatga gcgcggtgcg caaagtgctt tctgcgccgc agcaaaccca tcttattctg
    30481 gagtagtcgg tttttctgtc agttggctgt ggtactgccg gcgatattcc gacggcgagt
    30541 gttcggtgtt tttacgaaac agacggcaga agtagttgct gtcgacaaag ccgcaggcgt
    30601 gcgccacttc ttttaccttc aggtcatagc ctttcaataa cgtcttagcg tgctccagtc
    30661 gcgtgtgatt caggtattcg ttaaaaccaa tggcccccgt tttttgaaac aggtgcgaga
    30721 gatagtttgg tgaaatatag aatgcctgtg cgacagattc gcgggtaagc gcggaggcat
    30781 agcgttcgtc gatataatcg cgaatagctt caaatagtgc ctggctgcgt gaggcggtct
    30841 ggatttggct gccgcgcaaa tcgcggcagt ggctaagcag gctggctacg ataagccttg
    30901 ccgtttgctg ctcctgcggc tgcatttgca tttcattgag cgtttgtagc agaaaagaac
    30961 ctatgcgtgg gccgcgtcgg gcgacgtgtt gcttcgccag attttgatat tgtttgccat
    31021 cccattgcac gacgctgaag ccgagttgct gtttgccaaa cagcacgcta aaggtggtag
    31081 cgggtgtttt ccattgtggg aaattccagc caccagcggc aacgtacagc acatcgccgg
    31141 gaactaacgt tgctccagcg ccggtatcaa caaactcacc ttccagcaca atttccagcc
    31201 gtggaaaatc gacctgatac gcaagatcgg gcaccgggcc gttactgctg gcaaagtaaa
    31261 tctggcgcag ggacagcggg ccgttgatca ggcgggagag caaatagctg acgtcgtgat
    31321 acatgttatt cccttaggta tgtagacggg aaaacagtaa ctggcattat taaaaaaggc
    31381 cacctgaaaa gtgtggcctg aggggagttc gatgtaaaca ttgcggcagt atcgcatccg
    31441 gcaaatagcg ccagacgcta tacaaaggtc atctactgat tacccacctg atcgccatac
    31501 ggcagagtgt cgtaatcgat cagcgcgttt ttcttgtacg ggttaccaat ccagcgggta
    31561 gtttctttga actgcggatt caccactgaa cgggttggat cgtaaccgtc gtaagataag
    31621 cccgccaaat ctgaccaggt gtggatcagt tccgccaggc tgtatttacg atcgacatcc
    31681 tgtgagaaat cacggggatg agtcgcttgc catttttccg acgtccacag caggaacgga
    31741 atggtgtaca tgtggcgcgt cgggttgtct tcgttacgcc cctgagtttt gtgcggcggc
    31801 gtgtcgtaaa cctcttcacc gtggtcagag aagtaaacca ggaatccgtt cgggtctgcc
    31861 gctttaaagt ctttaatcag gctggcaacc acatgatcgt tatacaggtt agcgttgtca
    31921 taatcgttat atgattccag ctcttctgcg cttaatcctg gaggaacatg atcggtattg
    31981 ccatcaaact tgccctgatt ttccgggtag cggtatttgt atttgatatg cgtacccagc
    32041 agatgaacga tgatcagttt cttcggcgca gggtcattca gcacatcctg gaacggcttc
    32101 agcacgttgg tgtcgtattc acgcgcactc tgcgtacgtt gctggttcat gtagtactgc
    32161 ttgtcggtct ggcgcgaaaa taccgtcagc atggtattgc gggcggtcat cgtctgctgg
    32221 ttggtgatcc agaaggtttt ataacccgcc tgtttcatca tgttcatcag cgacggctgc
    32281 gtcagataca gatccgggtt cttttcattg gcaaaggtca gcgcctgttg caggatttca
    32341 atggtgtacg gacgagacgt aactacgtta ttaaacacgg tcagattcgg atcggtttta
    32401 tgcagcgcat ccagctccgg cgtggtttca cgcggataac cgtacagact catgcgtccg
    32461 cgctgggtcg actcgccaat caccagcact aaagtgcgcg gttcgttacc cgattcatct
    32521 ttgaaattag ccagtggcgg caaggcatta ttttcattca gtaactttgt tagcgagttt
    32581 agttgctgac gatactgata atagccggtc aggaattgcc acggtgcggc aggctccata
    32641 cgcgaggcta ggttatccaa cgttttctca aacggcttgt ttttgataaa cgtattcatg
    32701 gcgatcggat gcagaatcaa gccataaagc agggcaaaag agacaacata acgccacggc
    32761 tttggaatat agaccgggcg caggcgtgtc cacagcagaa ctgccaccgc cgtataggcc
    32821 agcgcgataa gcacaatttt caggctgaaa tactggctta aatactcgct ggcttcgttg
    32881 gtgttggttt cgaacatcac aaacagaacg ctctgcgaga actcctgccc gtagatgacg
    32941 tagtagcaca gcgccgccag agaggccgtc catagcacca cgccgatcac tgcggcaata
    33001 attttaatcc gcttcggaaa gaggaatacc gggatcaacc acagcgagct gaataacagc
    33061 gagtcgcgaa tgccgttggt gccactataa ccactgatgt aaataatggc ctgtagcaga
    33121 gtagagaaaa accaaaagta gagcagtgcc caacccaggg ctttccagct agaaaggggt
    33181 ttagcctgga cttctgtgga atgcatagta agaacctgtc ttgaaaaaaa tatcgccgaa
    33241 tgtaacgaca attccttaag gatatctgaa ggtatattca gaatttgaat aaaatgcaga
    33301 cagaaatata ttgaaaacga gggtgttaga acagaagtat ttcagaaaac cctcgcgcaa
    33361 aagcacgagg gtttgcagaa gaggaagatt agccggtatt acgcatacct gccgcaatcc
    33421 cggcaatagt gaccattaac gcttgttcga cgcgaggatc cggttcctgg ccttcttttt
    33481 ctgcctggcg ggagcggtgc agcaactcgg cctgcaatac gttcagcggg tcggtgtaaa
    33541 tattccgtag ctgaatagac tctgcaatcc acggcagatc ggccatcaga tgggaatcgt
    33601 tggcaatcgc cagcaccact ttgatgtctt cttcttgcag gttgcgtaac tctttaccta
    33661 acggccacag tgctttgtct accagacgtt gatcatagta ttccgccaac cacaggtctg
    33721 ctttggcgaa gaccatctcc agcatgccga gacgcgtcga gaagaatggc caatcgcggc
    33781 acatggcttc cagctcgctc tgtttgccat cttcgaccac tttttgcagc gccgtacctg
    33841 cacccagcca ggcggggagc atcaggcggt tttgcgtcca ggcaaaaatc cacggtatgg
    33901 cgcgcagcga ctcgacgccg ccggttgggc gacgtttcgc cggacgtgaa cccaacggca
    33961 gtttgcccaa ttcttgttcc ggcgtagcgg agcggaagta aggcacaaaa tctttgtttt
    34021 cacgtacgta gccacggtag agatcgcagg agatgactga cagttcatcc ataatgcgac
    34081 gccagctctc tttcggctca ggtggtggca gcaggttggc ttccagaatc gccccggtgt
    34141 aaagcgacag gctgctgacg gtgatttctg gcagaccata tttaaagcgg atcatctcgc
    34201 cctgttcagt cacgcgcagg ccacctttca ggcttcctgg cggttgtgac agcagcgccg
    34261 catgggcagg tgcgccgccg cgaccaatgg aaccgccgcg accgtggaac aacgtcagct
    34321 caatacccgc tttttcgcag gttttgatta atgcatcctg tgcctgatat tgcgcccagg
    34381 aagctgccat cacgcccgca tcttttgctg agtcggaata gccaatcatc accatctgtt
    34441 tgccctgaat caggccgcga taccagtcga tattgagcag ctgggtcatg acatcgttgg
    34501 cgttgttcag gtcatcgagg gtttcaaaca gcggagcaac cggcatcgca aacccgatac
    34561 ccgcttcttt cagcagcagg tggacagcca gtacgtcgga cggcgttttc gccatcgaga
    34621 tcacgtaggc ggcaatggag ccttgcggcg cttcggcaat cacctggcag gtatcgagca
    34681 cttcgcgcgt ttcggcgctt ggttgccagt tgcgcggcag aagcggacgt ttggagttca
    34741 gttcgcggat caggaacgcc tgtttgtcgg cctctgacca gctttcgtag tcgccgatac
    34801 cgaggtagcg ggtcagctcg cccagcgctt cggtatggcg ggtgctctcc tgacggatat
    34861 caatacggac cagcggtacg ccgaaacatt tcacgcggcg cagggtgtcg agcagatcgc
    34921 cgttggcgat aatacccatg ccacacgcct gaagtgactg gtagcaagcg tagagcggtt
    34981 cccacagttc ttcgttttgt gtcagcagcc cttctggttt tggcagttct tcgcctttca
    35041 ggcgcgcttc cagccatgcc tgtgtcgcca tcaggcgaga acgcaggttt ttcatcagat
    35101 agcgatacgg ttctgcggca ccttcttcgc caaccagcgc cagcagttca ggggtcgctt
    35161 caaccatcga cagttcagaa accagaacct gaatatcttt caggaacaga tcggtggctt
    35221 tccagcggct gagcagcagg acgtggcggg tgatatcggc agtgacgttc gggttgccgt
    35281 cgcggtcacc gcccatccac gaggtaaaac gtaccggaac aaattcgacg ggcagtttgt
    35341 agtcgagatt ctcttccagt tgttcgttca gttcgcgcag gtaattcggt acgccttgcc
    35401 acaggctgtt ttctactacg gcaaagcccc atttggcttc atctaccggg cttggacgca
    35461 gcttacggat ttcatcggta tgccatgact gggcgatcaa ctggtgcagg cgacgcatca
    35521 gctggttgtg ttcgtagtca gcgatatctt tgttatcgag ctgttttaaa caggcgttca
    35581 cttccaccat tttgtggatc agtgtacgac gggtaatttc ggttgggtga gccgtgagga
    35641 ccagttccag cgacagcgat tccactgctt ttttgatggt gtcttcgctc agttccggct
    35701 ggtttttcag tttacgcagg gtgcgggcga tcacttccgg gttgctggca gcttcgcctt
    35761 tcggcgaaat gctgtggtat tgctcggcgg tgttggccag gttcaggaac tgactaaacg
    35821 cacgcgcaac gggcagcagc tcgtcgttcg acaaattttg taaggtggtg agcaactcct
    35881 ggcggttagc atcattgcca gcgcgtgaag atttcgacaa cttacggata gtttctacgc
    35941 gttcaagaat gtgttctccc aacgcatcct tgatggtttc tcccagcact ttgccgagca
    36001 tactgacatt actacgcaat gcggaatatt gttcgttcat attaccccag acaccccatc
    36061 ttatcgtttg atagccctgt atccttcacg tcgcattggc gcgaatatgc tcgggctttg
    36121 cttttcgtcg tcttttataa agccacgtaa aagcggtgac gtcaaatgct gcgaaatcgc
    36181 ttcagcaaac gaataaatag caggaattta cgccattaaa ttcacgacgc tttaaataag
    36241 cgtaacttat ggaaatgtta aaaaatcgcc atacataaca ccaaaggtgt aggtcggata
    36301 agatgcgcaa gcatcgcatc cgacattatt gcggcaccgg agtttggcaa cggtgccgga
    36361 tgcggcgcga gcgccttatc cggcctacgt tttaatgcca gcaaaaatgg tgaattacct
    36421 ggataatcaa ctcacgggtg ggtttaataa accgcgtctc cagatattca tcaggctgat
    36481 gagcttgatt gatggaaccc ggaccgagca ccagcgtcgg gcataacgtt tgaataaacg
    36541 gcgcttcggt acagtaattc accacttcgg tttttgctcc gagcaatttc tcaaccactt
    36601 caaccagttg atgattcggt gggcattcat agccagggat cggcggatgc agctcgtcga
    36661 ccgtcagacg acccggccag cgttcgctca ccggagccaa tgcatcgttg agcaaaccat
    36721 taagttcatt gagtgtcatg ccaggctgcg gacgaatatc catatgcaac tcacagcaag
    36781 cgcaaatacg gttagaagcg tcgccaccgt gaatatgccc gaggttgagc gtagggtatg
    36841 gcacggtaaa tgcttcgtag tgataacgtt ctttcaggtt atcgcgcaat tgcaaaatat
    36901 gcccgatggc gtcgtgcatt agttcgatag cgttaactcc gcgtgctgga tcgctggagt
    36961 gccccgactg gccctgaata cggatggcgt tagagatatg acctttatgt gcgcgtaccg
    37021 gttgtagtga cgtcggttcg ccaatgatgg cgcaatccgg gcgcagggcg gtagtttcgg
    37081 caaaataacg cgctccggcc atactggttt cttcatcagc agtcgccaga atgtagagcg
    37141 gttttttcag tttcgtgacg tcgacatcgc gtagcgcatc aaggataaac gcaaaaaagc
    37201 ctttcatgtc ggcggtgcct aagccgtaaa gcttgccgtc atgctccgtc agtgtaaacg
    37261 gatcgcgcgt ccagcgaccg tcatcaaatg gcaccgtatc ggtatgcccc gccagcaaca
    37321 agccgccagc cccctgtccg taactggcca gcatattaaa tttgttgcga gttcctggaa
    37381 caggctgcac ttccacattg aagcccaaat ctttaaacca gtccgccagc agagtgatta
    37441 aatctgcatt gctttgatcg agtgcctctt ccgtagcgct tattgaaggt gtggcaatca
    37501 gagcgcggta aatctcgata aatggcggta atttgttttt cattgttgac acacctctgg
    37561 tcatgatagt atcaatattc atgcagtatt tatgaataaa aatacactaa cgttgagcgt
    37621 aataaaaccc accagccgta aggtgaatgt tttacgttta acctggcaac cagacataag
    37681 aaggtgaata gccccgatgt tgaatacgct gattgtgggt gccagcggct acgctggcgc
    37741 agagctagtg acctatgtaa atcgccatcc gcatatgaac ataaccgctt tgactgtttc
    37801 agcgcaaagc aatgatgcgg gaaagttaat ctccgatttg catccgcagc taaaaggcat
    37861 cgttgagctg ccgttgcagc cgatgtcgga tatcagcgag tttagcccag gggtggacgt
    37921 agtgtttctc gccaccgccc atgaagttag ccacgattta gcgccgcagt ttcttgaagc
    37981 gggctgcgtg gtgttcgacc tttccggcgc gtttcgtgtt aacgacgcca ccttctatga
    38041 aaaatattac ggctttaccc atcaataccc ggaactgctg gaacaggcag cctacggtct
    38101 ggcggagtgg tgcggtaata aattaaaaga agcgaatttg attgcggtgc caggctgtta
    38161 tccgacggcg gcacagctgg cgctgaaacc gttgattgat gccgatcttc ttgacctcaa
    38221 tcagtggccg gtgatcaacg ccaccagcgg cgtgagcggt gcaggacgta aggcagcgat
    38281 ttcaaatagt ttttgtgaag ttagcctgca accgtatggc gtctttactc atcgccatca
    38341 accagagatc gccacacacc tcggtgctga cgttatcttc accccacatc tgggcaattt
    38401 cccgcgcggc attctcgaaa ccattacctg ccgcctgaaa tcgggtgtga cccaggcgca
    38461 agtcgcgcaa gcgttacagc aggcgtatgc ccataaaccg ctggtgcggc tgtatgacaa
    38521 aggcgttccg gcgctgaaaa atgtcgttgg gctgccattt tgcgatatcg ggtttgccgt
    38581 tcagggcgag catctgatta ttgtggcgac cgaagacaac ttactgaaag gcgcggcggc
    38641 acaagcggta cagtgcgcca atattcgttt cggctatgcg gaaacgcagt ctcttattta
    38701 agggtgcaat gatgaatcca ttaattatca aactgggcgg cgtactgctg gatagtgaag
    38761 aggcgctgga acgtctgttt agcgcattgg tgaattatcg tgagtcacat cagcgtccgc
    38821 tggtgattgt gcacggcggc ggttgcgtgg tggatgagct gatgaaaggg ctgaatctgc
    38881 cggtgaaaaa gaaaaacggc ctgcgggtga cgcctgctga tcagatagac attatcaccg
    38941 gagcactggc gggaacggca aataaaaccc tgttggcatg ggcgaagaaa catcagattg
    39001 cggccgtagg tttgtttctc ggtgacggcg acagcgtcaa agtgacccag cttgatgaag
    39061 agttaggtca tgttggactg gcgcagccag gttcgcctaa gcttatcaac tccttgctgg
    39121 agaacggtta tctgccggtg gtcagctcca ttggcgtaac agacgaaggg caactgatga
    39181 acgtcaatgc cgaccaggcg gcaacggcgc tggcggcaac gctgggcgcg gatctgattt
    39241 tgctctccga cgtcagcggc attctcgacg gcaaagggca acgcattgcc gaaatgaccg
    39301 ccgcgaaagc agaacaactg attgagcagg gcattattac tgacggcatg atagtgaaag
    39361 tgaacgcggc gctggatgcg gcccgcacgc tgggccgtcc ggtagatatc gcttcttggc
    39421 gttatgcgga gcagcttccg gcactgttta acggtatgcc gatgggtacg cggattttag
    39481 cttaagtttt gttagccgga ggagcagctt tccggcattg aatttcaaaa taaggaaaca
    39541 gagttatggc actttggggc gggcgtttta cccaggcagc agatcaacgg ttcaaacaat
    39601 tcaacgactc actgcgcttt gattaccgtc tggcggagca ggatattgtt ggctctgtgg
    39661 cctggtccaa agccttggta acggtcggcg tgttaaccgc agaagagcag gcgcaactgg
    39721 aagaggcgct gaacgtgttg ctggaagatg ttcgcgccag gccacaacaa atccttgaaa
    39781 gcgacgccga agatatccat agctgggtgg aaggcaaact gatcgacaaa gtgggccagt
    39841 taggcaaaaa gctgcatacc gggcgtagcc gtaatgatca ggtagcgact gacctgaaac
    39901 tgtggtgcaa agataccgtt agcgagttac tgacggctaa ccggcagctg caatcggcgc
    39961 tggtggaaac cgcacaaaac aatcaggacg cggtaatgcc aggttacact cacctgcaac
    40021 gcgcccagcc ggtgacgttc gcgcactggt gcctggccta tgttgagatg ctggcgcgtg
    40081 atgaaagccg tttgcaggat gcgcttaagc gtctggatgt cagcccatta ggctgtggcg
    40141 cgctggcggg aacggcctat gaaatcgacc gtgaacagtt agcaggctgg ctgggctttg
    40201 cttcggcgac ccgtaacagt ctggacagtg tttctgaccg tgaccatgtg ttggaactgc
    40261 tttctgctgc cgctatcggc atggtgcatc tgtcgcgttt tgctgaagat ctgattttct
    40321 ttaacaccgg cgaagcgggg tttgtggagc tttcggaccg cgtgacttcc ggttcatcat
    40381 taatgccgca gaagaaaaac ccggatgcgc tggagctgat tcgcggtaaa tgcggccggg
    40441 tgcagggtgc gttaaccggc atgatgatga cgctgaaagg tttgccgctg gcttacaaca
    40501 aagatatgca ggaagacaaa gaaggtctgt tcgacgcgct cgatacctgg ctggactgcc
    40561 tgcatatggc ggcgctggta ctggacggca ttcaggtgaa acgtccgcgt tgtcaggaag
    40621 cggcgcagca ggggtatgcg aacgccactg aactggcgga ttacctggtg gcgaaaggcg
    40681 tacctttccg cgaggcgcac catattgtgg gtgaagcggt ggtggaagcc attcgtcagg
    40741 gcaaaccgct ggaagatctg ccactcagtg agttgcagaa attcagtcag gtgattggcg
    40801 aagatgtcta tccgattctg tcgctacaat cgtgcctcga aaagcgtgcg gcaaaaggcg
    40861 gcgtctcacc gcagcaggtg gcgcaggcga ttgcttttgc gcaggctcgg ttagagtaat
    40921 ttgtttatat tgctcccctg aaaatgggga gtttaccacg cctacaaagt gcgacacttc
    40981 gtaggcggat aaggcgttta cgccgcatca ggcagaaatt tgtcagcatt c
//
LOCUS       DADMGC010000019        38753 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.019, whole genome shotgun sequence.
ACCESSION   DADMGC010000019 DADMGC010000000
VERSION     DADMGC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.019"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..246)
                     /gene="yefM"
                     /locus_tag="JNZ73_001019"
     CDS             complement(<1..246)
                     /gene="yefM"
                     /locus_tag="JNZ73_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HAY8731389.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            529..579
                     /gene="hisL"
                     /locus_tag="JNZ73_001020"
     CDS             529..579
                     /gene="hisL"
                     /locus_tag="JNZ73_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HAY8731390.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            725..1624
                     /gene="hisG"
                     /locus_tag="JNZ73_001021"
     CDS             725..1624
                     /gene="hisG"
                     /locus_tag="JNZ73_001021"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAY8731391.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1630..2934
                     /gene="hisD"
                     /locus_tag="JNZ73_001022"
     CDS             1630..2934
                     /gene="hisD"
                     /locus_tag="JNZ73_001022"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAY8731392.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2931..4001
                     /gene="hisC"
                     /locus_tag="JNZ73_001023"
     CDS             2931..4001
                     /gene="hisC"
                     /locus_tag="JNZ73_001023"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAY8731393.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            4001..5068
                     /gene="hisB"
                     /locus_tag="JNZ73_001024"
     CDS             4001..5068
                     /gene="hisB"
                     /locus_tag="JNZ73_001024"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HAY8731394.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KEGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5068..5658
                     /gene="hisH"
                     /locus_tag="JNZ73_001025"
     CDS             5068..5658
                     /gene="hisH"
                     /locus_tag="JNZ73_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAY8731395.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5658..6395
                     /gene="hisA"
                     /locus_tag="JNZ73_001026"
     CDS             5658..6395
                     /gene="hisA"
                     /locus_tag="JNZ73_001026"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAY8731396.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGISLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6377..7153
                     /gene="hisF"
                     /locus_tag="JNZ73_001027"
     CDS             6377..7153
                     /gene="hisF"
                     /locus_tag="JNZ73_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAY8731397.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7147..7758
                     /locus_tag="JNZ73_001028"
     CDS             7147..7758
                     /locus_tag="JNZ73_001028"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAY8731398.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7944..8921)
                     /gene="wzzB"
                     /locus_tag="JNZ73_001029"
     CDS             complement(7944..8921)
                     /gene="wzzB"
                     /locus_tag="JNZ73_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HAY8731399.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9067..10233)
                     /gene="ugd"
                     /locus_tag="JNZ73_001030"
     CDS             complement(9067..10233)
                     /gene="ugd"
                     /locus_tag="JNZ73_001030"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HAY8731400.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEDYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRTSSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(10483..11889)
                     /gene="gndA"
                     /locus_tag="JNZ73_001031"
     CDS             complement(10483..11889)
                     /gene="gndA"
                     /locus_tag="JNZ73_001031"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAY8731401.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11998..12357)
                     /locus_tag="JNZ73_001032"
     CDS             complement(11998..12357)
                     /locus_tag="JNZ73_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731402.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12354..12906)
                     /locus_tag="JNZ73_001033"
                     /pseudo
     CDS             complement(12354..12906)
                     /locus_tag="JNZ73_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13007..13606)
                     /locus_tag="JNZ73_001034"
     CDS             complement(13007..13606)
                     /locus_tag="JNZ73_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731403.1"
                     /translation="MSLNILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLA
                     INNIEFSTLFVILFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYL
                     RFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPSIIAT
                     ILLFTINPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13778..14716)
                     /locus_tag="JNZ73_001035"
                     /pseudo
     CDS             complement(13778..14716)
                     /locus_tag="JNZ73_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14779..15153)
                     /locus_tag="JNZ73_001036"
     CDS             complement(14779..15153)
                     /locus_tag="JNZ73_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY8731404.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFELLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15157..16305)
                     /gene="wzy"
                     /locus_tag="JNZ73_001037"
     CDS             complement(15157..16305)
                     /gene="wzy"
                     /locus_tag="JNZ73_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HAY8731405.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFLCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16325..17194)
                     /locus_tag="JNZ73_001038"
     CDS             complement(16325..17194)
                     /locus_tag="JNZ73_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY8731406.1"
                     /translation="MATYNGECWIEEQLKSIIEQKDVDISIFISDDLSTDNTLNICEE
                     FQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQDDIWYKDKIKNAI
                     DCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPGCTYVIKKETLIE
                     FKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQHENNQVGANISFK
                     AKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILALSFWKLRRKKAD
                     KIYILLMLILNIF"
     gene            complement(17223..18113)
                     /locus_tag="JNZ73_001039"
     CDS             complement(17223..18113)
                     /locus_tag="JNZ73_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HAY8731407.1"
                     /translation="MNSNIYAVIVTYNPELKNLNELITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18143..19399)
                     /gene="wzx"
                     /locus_tag="JNZ73_001040"
     CDS             complement(18143..19399)
                     /gene="wzx"
                     /locus_tag="JNZ73_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HAY8731408.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19415..19960)
                     /gene="rfbC"
                     /locus_tag="JNZ73_001041"
     CDS             complement(19415..19960)
                     /gene="rfbC"
                     /locus_tag="JNZ73_001041"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAY8731409.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19965..20843)
                     /gene="rfbA"
                     /locus_tag="JNZ73_001042"
     CDS             complement(19965..20843)
                     /gene="rfbA"
                     /locus_tag="JNZ73_001042"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY8731410.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20902..21801)
                     /gene="rfbD"
                     /locus_tag="JNZ73_001043"
     CDS             complement(20902..21801)
                     /gene="rfbD"
                     /locus_tag="JNZ73_001043"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAY8731411.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21801..22886)
                     /gene="rfbB"
                     /locus_tag="JNZ73_001044"
     CDS             complement(21801..22886)
                     /gene="rfbB"
                     /locus_tag="JNZ73_001044"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY8731412.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23259..24152)
                     /gene="galF"
                     /locus_tag="JNZ73_001045"
     CDS             complement(23259..24152)
                     /gene="galF"
                     /locus_tag="JNZ73_001045"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HAY8731413.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24327..25721)
                     /gene="wcaM"
                     /locus_tag="JNZ73_001046"
     CDS             complement(24327..25721)
                     /gene="wcaM"
                     /locus_tag="JNZ73_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HAY8731414.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLNHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFIRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25732..26952)
                     /gene="wcaL"
                     /locus_tag="JNZ73_001047"
     CDS             complement(25732..26952)
                     /gene="wcaL"
                     /locus_tag="JNZ73_001047"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HAY8731415.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSYEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26949..28229)
                     /gene="wcaK"
                     /locus_tag="JNZ73_001048"
     CDS             complement(26949..28229)
                     /gene="wcaK"
                     /locus_tag="JNZ73_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HAY8731416.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRVKKVLRRSYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMIADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28409..29887)
                     /gene="wzxC"
                     /locus_tag="JNZ73_001049"
     CDS             complement(28409..29887)
                     /gene="wzxC"
                     /locus_tag="JNZ73_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-antigen undecaprenyl disphosphate flippase"
                     /protein_id="HAY8731417.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVSVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29889..31283)
                     /gene="wcaJ"
                     /locus_tag="JNZ73_001050"
     CDS             complement(29889..31283)
                     /gene="wcaJ"
                     /locus_tag="JNZ73_001050"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HAY8731418.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDTKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLAHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGGNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31338..32708)
                     /gene="cpsG"
                     /locus_tag="JNZ73_001051"
     CDS             complement(31338..32708)
                     /gene="cpsG"
                     /locus_tag="JNZ73_001051"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HAY8731419.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32813..34249)
                     /gene="cpsB"
                     /locus_tag="JNZ73_001052"
     CDS             complement(32813..34249)
                     /gene="cpsB"
                     /locus_tag="JNZ73_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HAY8731420.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADKDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASSEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGHHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34252..35475)
                     /gene="wcaI"
                     /locus_tag="JNZ73_001053"
     CDS             complement(34252..35475)
                     /gene="wcaI"
                     /locus_tag="JNZ73_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HAY8731421.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLLFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35472..35951)
                     /locus_tag="JNZ73_001054"
     CDS             complement(35472..35951)
                     /locus_tag="JNZ73_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HAY8731422.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35951..36919)
                     /gene="fcl"
                     /locus_tag="JNZ73_001055"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35951..36919)
                     /gene="fcl"
                     /locus_tag="JNZ73_001055"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAY8731423.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36922..38043)
                     /gene="gmd"
                     /locus_tag="JNZ73_001056"
     CDS             complement(36922..38043)
                     /gene="gmd"
                     /locus_tag="JNZ73_001056"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAY8731424.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38069..38617)
                     /gene="wcaF"
                     /locus_tag="JNZ73_001057"
     CDS             complement(38069..38617)
                     /gene="wcaF"
                     /locus_tag="JNZ73_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HAY8731425.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38633..>38753)
                     /locus_tag="JNZ73_001058"
     CDS             complement(38633..>38753)
                     /locus_tag="JNZ73_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyl transferase"
                     /protein_id="HAY8731426.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10349 a         9687 c         8721 g         9996 t
ORIGIN      
        1 aataatgtcc ttttccgttc ctttgcctga tttcaggcta tcgattgagt ccatcaatct
       61 ccgggcgtta gcgggggaac gcagtagata agccgtctct tccagcgagt tgtattcttc
      121 gagtgacatc agaacacaag cctctccatt ctgacgagta atgaggatcg gggcatgatc
      181 ttcaacggct ttcatcattg ttgccgacaa attctgacgc gcttcgctgt agctaattgt
      241 acgcatgtaa atctcctatt ttgtacagtt cattgtacaa tgataggtgt taattaacta
      301 tttattaatt agtttgtaga tcaagacatt gccagcgaga cgaaaaatca gactttgctc
      361 tttttggtgc tgtcaagtta gaggacagtc ctcttagccc cctcctttcc ccgctcattc
      421 attaaacaaa tccatcgcca taaaatatat aaaaaagccc ttgctttcta acgtgaaagt
      481 ggtttaggtt aaaagacatc agttgaataa acattcacag agatttttat gacacgcgtt
      541 caatttaaac accaccatca tcaccatcat cctgactagt ctttcaggcg atgtgtgctg
      601 gaagacattc agatcttcca gtggtgcatg aacgcatgag aaagcccccg gaagatcacc
      661 ttccgggggc ttttttattg cgcggttgtt aacggttcag acaggtttaa agaggaataa
      721 gaaaatgaca gacaacactc gtttacgcat agctatgcag aaatccggcc gtttaagtga
      781 tgactcacgc gaattactgg cgcgctgtgg cattaaaatc aatcttcaca cccagcgcct
      841 gatcgcgatg gcagaaaaca tgccgattga tattctgcgc gtgcgtgacg atgacattcc
      901 cggtctggta atggacggcg tggtagacct tgggattatc ggcgaaaacg tgctggaaga
      961 agagctgctt aaccgccgcg cccagggtga agatccgcgc tactttaccc tgcgtcgtct
     1021 ggatttcggc ggctgccgcc tttcgctggc aacgccggtt gatgaagcct gggacggtcc
     1081 gctctcctta aacggtaaac gtatcgccac ctcttatcct cacctgctca agcgttatct
     1141 cgaccagaaa ggtatctctt ttaaatcctg cttactgaac ggttctgtcg aagttgcgcc
     1201 gcgtgccggg ctggcggatg cgatttgcga tctggtttcc accggtgcca cgctggaagc
     1261 taacggcctg cgcgaagtcg aagttatcta ccgctcgaaa gcctgcctga tccagcgcga
     1321 tggcgaaatg gaagaatcta aacagcaact gatcgacaag ctactgaccc gtattcaggg
     1381 tgtgatccag gcgcgcgaat caaaatacat catgatgcac gcaccgaccg aacgcctaga
     1441 tgaagtcatc gccctgctgc caggtgccga acgcccaacc attctgccgc tggcgggtga
     1501 tcaacagcgc gtagcgatgc acatggtgag tagcgaaacc ctgttctggg aaacgatgga
     1561 aaaactgaaa gcactgggtg ccagttcaat cctggtcctg ccgattgaga agatgatgga
     1621 gtgatcgcca tgagctttaa cacaatcatt gactggaata gctgtactgc ggagcaacaa
     1681 cgccagctgt taatgcgccc ggcgatctcc gcttctgaaa gcattacccg caccgttaac
     1741 gatattctcg ataacgtgaa aacgcgtggc gatgaggccc tgcgtgaata cagcgcgaag
     1801 tttgataaaa ccacggttac cgcgctgaag gtgtctgctg atgagatcgc cgccgccagc
     1861 gaacgcctga gcgacgagct aaaacaggcg atggcggtgg cagtaaagag tattgaaacc
     1921 ttccacactg cgcaaaaact gccgccggta gatgtagaaa cgcagccagg cgtgcgttgc
     1981 cagcaggtca cgcgcccggt ggcttcagtg ggtttgtata ttccgggcgg ctccgccccg
     2041 ctcttctcaa cggtattaat gctggcaact ccggcgcgta ttgcgggctg taaaaaagtg
     2101 gtgctgtgct caccgccgcc gattgccgat gaaattcttt atgcggcgca gctgtgcgat
     2161 gtgcaggacg tgtttaacgt cggcggcgca caggccattg ccgcgctggc gtttggtacg
     2221 gaatctgtgc cgaaagtgga caaaatcttc gggccgggta acgcctttgt caccgaagca
     2281 aaacgccagg taagccagcg tctggacggt gcggcgatcg atatgcccgc aggcccgtcg
     2341 gaagtgctgg tgattgctga cagcggcgct acgccggatt ttgtggcttc tgatttgctt
     2401 tctcaggctg aacacggccc tgactcacag gtaattttac tgacccccga tgctgatatg
     2461 gcgcgtcgcg ttgccgaggc cgtcgaacgc caactggcag aactgccgcg tgccgaaacc
     2521 acccgccagg cactgaacgc cagccgcctg atcgtgacta aagatttagc gcagtgcgtg
     2581 gagatctcca accagtacgg cccggagcac ctgatcattc agacccgcaa cgcccgcgaa
     2641 ctggtcgatg gcatcaccag cgccggttcg gtatttcttg gtgactggtc accggaatcg
     2701 gcaggcgact acgcctccgg caccaaccac gtgctgccga cttacggtta caccgccacc
     2761 tgttccagcc tcgggctggc agatttccag aaacgcatga ccgtacagga actgtcgaaa
     2821 gaaggtttct ccgcgctggc ttcgaccatt gaaacactgg ccgccgccga gcgcctgacc
     2881 gcccacaaaa atgccgttac tttgcgtgtt aacgtcctta aggagcaagc atgagcaccg
     2941 tgactattac tgatttagcg cgtgaaaacg tccgcaacct gacgccgtat cagtcggcgc
     3001 gtcgtctggg cggtaacggc gacgtctggc tgaacgccaa cgaatatccc acagccgtgg
     3061 agtttaagct tactcagcaa acgctcaacc gctacccgga atgccagcca aaagcggtga
     3121 ttgaaaatta cgcgcaatat gcaggcgtaa aaccggaaca ggtgctggtc agccgtggtg
     3181 cggacgaagg tattgaactg ctgatccgcg ctttttgcga accgggtaaa gacgccatcc
     3241 tctactgccc gccaacgtac ggcatgtaca gcgtcagcgc tgaaaccatt ggtgtcgaat
     3301 gccgcacagt gccgacgctg gaaaactggc aactggactt acagggcatt tccgacaagc
     3361 tggacggcgt aaaagtggtc tatgtttgca gccccaacaa ccccaccggg caactgatca
     3421 atccgcagga ttttcgcacc ctgctggagc taacccgcgg taaggcgatt gtggttgccg
     3481 atgaagctta tatcgagttt tgcccgcagg catcgttagc tggctggctg gcggaatatc
     3541 cgcacctggc gattttgcgt accctttcga aagccttcgc gttggcgggg cttcgttgcg
     3601 gatttacgct ggcaaacgaa gaagtcatca acctgctgat gaaagtgatc cccccctacc
     3661 cgctctcgac gccggttgcc gacattgccg cacaggcgtt aagcccgcag ggaatcgtcg
     3721 ccatgcgcga acgggtggcg caaattatta cagaacgcga atacctgatt gccgcactga
     3781 aagagatccc ctgcgtggag caggttttcg actctgaaac caactacatt ctggcgcgct
     3841 ttaaagcctc cagtgcagtg tttaaatctt tgtgggatca gggcattatc ttacgtgatc
     3901 agaataaaca accctcttta agcggctgcc tgcgaattac cgtcggaacc cgtgaagaaa
     3961 gccagcgcgt cattgacgcc ttacgtgcgg agcaagtttg atgagtcaga agtatctttt
     4021 tatcgatcgc gatggaaccc tgattagcga accgccgagt gattttcagg tggaccgttt
     4081 tgacaaactc gcctttgaac cgggcgtgat cccggaattg ctgaagctgc aaaaagaggg
     4141 ctacaagctg gtgatgatca ctaatcagga tggtctggga acacaaagtt tcccgcaagc
     4201 ggattttgat ggcccgcaca acctgatgat gcagatcttc acctcgcaag gcgtgcagtt
     4261 tgatgaagtg ctgatttgtc cgcacctgcc cgccgatgag tgcgactgcc gtaagccgaa
     4321 agtaaaactg gtggagggtt atctggctga gcaagcgatg gatcgtgcca acagttatgt
     4381 aattggcgat cgcgcgaccg acattcaact ggcggaaaac atgggcatta atggtttacg
     4441 ctacgaccgc gaaaccctga actggccaat gattggcgag caactcacta aacgagaccg
     4501 ttacgcccat gtagtgcgca acaccaaaga gacgcagatt gacgtccagg tgtggctgga
     4561 tcgcgaaggt ggcagcaaga ttaacaccgg cgttggcttc tttgatcaca tgctggatca
     4621 gatcgccacc cacggcggtt tccgcatgga aatcaatgtc aaaggcgacc tctatatcga
     4681 cgatcaccac accgtcgaag ataccggcct ggcgctgggc gaagcgttaa aaattgccct
     4741 cggcgacaaa cgcggtattt gccgctttgg ttttgtgctg ccgatggacg aatgccttgc
     4801 ccgctgcgca ctggatatct ctggtcgccc gcacctggaa tataaagccg agtttaccta
     4861 ccagcgtgtg ggcgatctca gcactgaaat gatcgaacac ttcttccgtt cgctctctta
     4921 caccatgggc gtgacactac acctgaaaac caaaggtaaa aacgatcatc accgtgtaga
     4981 aagcctgttc aaagcctttg gtcgtaccct gcgccaggcc atccgcgtcg aaggcgacac
     5041 cctgccctcg tcgaaaggag tgctgtaatg aacgtggtga tccttgatac tggctgcgcc
     5101 aacctgaact ctgtgaagtc tgccattgcg cgccacggtt atgaacccaa agtcagccgt
     5161 gacccggaca ttgtgttgct ggccgataaa ctgtttttac ccggcgtcgg caccgcacaa
     5221 gcagcgatgg atcaggtgcg tgagcgcgag ctgtttgatc tcatcaaagc ctgtacccaa
     5281 ccggtgctgg gtatctgctt agggatgcaa ctcctgggac gacgcagcga agagagcaat
     5341 ggcgtcgact tgctgggcat catcgacgaa gacgtgccga aaatgaccga ctttggtctg
     5401 ccactgccac atatgggctg gaaccgcgtt tatccgcagg caggcaaccg cctgtttcag
     5461 gggattgaag acggcgcgta cttttacttt gttcacagct acgcaatgcc agtcaatccg
     5521 tggaccatcg cccagtgtaa ttacggcgaa ccgttcaccg cggcggtaca aaaagataac
     5581 ttctacggcg tgcagttcca cccggagcgt tctggtgccg ctggcgctaa gttgctgaaa
     5641 aacttcctgg agatgtgatg attattccgg cattagattt aatcgacggc accgtggtgc
     5701 gtctccatca gggcgattac ggcaaacaac gcgattacgg taacgacccg ctgccgcgct
     5761 tacaggatta cgctgcgcaa ggtgccgaag tgttgcacct ggtggatctg accggggcaa
     5821 aagatccggc taagcgtcaa atcccgctga ttaaaaccct ggtcgcgggc gttaacgtcc
     5881 cggtgcaggt tggcggcggt gttcgtagtg aagaagacgt ggcggcgtta ctggaagctg
     5941 gcgttgcgcg cgtggtggtc ggttccaccg cagtgaaatc accggacgta gtgaaaggct
     6001 ggtttgaacg tttcggtgcc gatgcgctgg tgctggcact ggatgtccgt attgacgagc
     6061 aaggcaacaa gcaagtggca gtcagcggct ggcaagagaa ctcgggcatt tcactggaac
     6121 aactggtgga aacctatctg cccgtcggcc tgaaacatgt gttgtgtacc gatatctcgc
     6181 gcgacggcac gctggcggga tctaacgtct ctttatatga agaagtgtgt gccagatatc
     6241 cgcaagtggc gtttcagtcc tccggcggca ttggcgacat taacgatgtt gctgccctac
     6301 gtggcactgg cgtacgcggc gtaatagttg gtcgtgcatt actggaaggt aaattcaccg
     6361 tgaaggaggc catcgcatgc tggcaaaacg cataatcccg tgtctcgacg ttcgtgatgg
     6421 tcaggtggtg aaaggcgtac agtttcgcaa ccatgaaatc attggcgata tcgtgccact
     6481 ggcaaaacgc tacgccgaag aaggtgcaga tgaactggtg ttctacgata tcaccgcttc
     6541 cagcgatggc cgtgtggtag ataaaagctg ggtatctcgc gtggcggaag tgatcgacat
     6601 tccgttttgt gtggcgggtg ggattaagtc tctggaagat gccgcgaaaa ttctttcctt
     6661 tggcgcggat aaaatttcca tcaactcccc tgcgctggca gacccaacat taattactcg
     6721 cctggccgat cgctttggcg tgcagtgtat tgtggttggt attgatacct ggtacgacgc
     6781 cgaaaccggt aaatatcatg taaatcaata taccggcgat gaaagccgca cccgcgtcac
     6841 tcaatgggaa acgctcgact gggtagagga ggtgcaaaaa cgcggcgccg gagaaatcgt
     6901 cctcaacatg atgaatcagg acggcgtgtg taacggttac gacctcaagc agctgaaaaa
     6961 agtgcgtgaa gtttgccacg tcccgctgat tgcctccggt ggcgcgggca ccatggaaca
     7021 cttcctggaa gccttccgcg atgccgacgt tgacggtgcg ctggcagctt ccgtattcca
     7081 caaacaaata atcaatattg gcgaattaaa agcgtacctg gcaacacagg gcgtggagat
     7141 caggatatgt taacagaaca acaacgtcgc gaactggact gggaaaaaac cgacggtctg
     7201 atgccggtga ttgtgcaaca cgcggtatct ggcgaagtgt taatgctggg ctacatgaac
     7261 ccggaagcct tagacaaaac catcgaaagc ggtaaagtca ccttcttctc gcgcactaaa
     7321 cagcgactgt ggaccaaagg cgaaacctcg ggcaattttc tcaatgtggt aagtattgcc
     7381 ccggactgcg acaacgacac attactggtg ctggcgaatc ccatcgggcc aacctgccac
     7441 aaaggcacca gtagctgctt cggcaacacc gctcaccagt ggctgttcct gtatcaactg
     7501 gaacaactgc tcgccgaacg taaatatgcc gacccggaaa cctcctacac cgccaaactg
     7561 tatgccagcg gcactaaacg cattgcgcag aaagtgggcg aagaaggcgt ggaaaccgca
     7621 ctggcagcaa cggtacatga tcgctttgag ctgaccaacg aggcatctga cttgatgtat
     7681 cacctgctgg tgctgttgca ggatcaggat ctggatttaa cgacagtaat tgagaacctg
     7741 cataaacggc atcagtgagt tacgggctga gcaggtgtga tgttgttgtc acacccggag
     7801 ccattgcccg atgcgcaaac ttatcgtgcc tacacattat ctcaacacac gtaggccgga
     7861 taagatgcgc cagcatcgca tccggcaaaa aaaacgggca tggtgtcacc accctgcccg
     7921 tttctcttaa atgcacaata atattacttc gcgttgtaat tacgcagagc gttacgcccc
     7981 aacacaatcc ccgcgccaac catgccgccc agaagcactg ccagaatcaa ggtgattgcc
     8041 tttttcggac tatcgcgacg aataggtaac gtcggtttca tcacatagcg gtaagcatga
     8101 atatcaagat catcaaactt taatttttca atatccaaca ggttttgacg tgtctgatag
     8161 tagtttggtg agaacaccaa cggacgagtc gcttcatgct taatcatcga ctccagcgct
     8221 tcgctcccta gaaggaacaa cgtatcttgc gtcacatctt cagtctgctg aacctgtggc
     8281 tttgtcacct gcgcctgatt cgcatactgc aacgcttcct gaatctgacg gatacgcaga
     8341 tctttctgct cctgcgcgac cacttcctgg gttcttaaag agtcctgcaa gtttttccgt
     8401 cccagagcaa tgttgtcctt gagatccttt tctagctctt gattcacttt atcatcaact
     8461 tgctgaatgt attgggccaa cttcatttgt gcgccctctg cagtttgccc aacataagaa
     8521 acagtcaatg gtaattgttg gttcttaaca gaaggttcga tggtaagttt ttctggctct
     8581 tcctgattat ccagcgtttc tgctaatgca gagaaggcag aactgaagcg accaattaac
     8641 gtctcctgca aatccgatac tttcggtgca gcctgaccat agataacatt catggcattg
     8701 ttatagccag caatttgccc cacgtcgggc tgagtgataa ttgctgttga cgtccatttc
     8761 tccttcgcta ctgccaaata accaatagcc agggcaatag ccacaatgac ggaaatgata
     8821 attgtcatct tgccacgcca caactgcact agtaaatcaa tcaaatcaat ctgttccggg
     8881 tcatggtttt gcccagaaac attattattt tctactctca tcattaccct aactgacgaa
     8941 aatttccaag tccgcatagt ttagcgataa tccccgtgaa tgctatagga taagtgataa
     9001 aaataatgaa ttattgcgga ggtaacaaaa gacaatcagg gcgtaaatag ccctgataac
     9061 aagatgttag tcgctgccaa agagatcgcg ggtgtagacc ttatctgcca catccttaag
     9121 ctcttctgcc atacggttgg agataatgac gtcggcttgt tgtttgaagg tggcgagatc
     9181 acgttccagg cgagagttga agaatgagtc ttctttcatc accggctcgt agatgatcac
     9241 ttcaacgcct ttcgccttga tacgcttcat aatcccctga atagaagacg tacggaagtt
     9301 atctgacccg ctcttcataa tcagacgata aatacccacc acttgcggtt tgcgtgacaa
     9361 aatggcatcg gcaataaaat ctttacgcgt gcggtttgca tcgacaattg ccgagatcag
     9421 gttattcggc acagactggt agttcgccag taactgcttg gtatctttcg gcagacaata
     9481 accaccataa ccaaacgacg gattgttgta atggttgcca atacgcggat cgagacaaac
     9541 gccttcgata atctggcgag tattcagacc taaactttct gcataactat ccagttcatt
     9601 aaagtatgct acgcgcatcg ccagataggt attagcgaaa agtttaatcg cttctgcttc
     9661 agtggagtcg gtaaacaggg tcgggatatt ttgcttaatc gcgccttcct gtaacaacgc
     9721 agcaaaacgt tctgcgcgtt ctgaacgctc accgatgaca atacgtgacg gatggagatt
     9781 atcgtaaagg gctttacctt cgcggaggaa ttccggagag aaaataatat tttcagtacg
     9841 atatttctta tgcatcgctg cggtaaaacc aacgggaacc gttgatttga tgaccataac
     9901 cgcataagga tttatctcaa ctacgtcttt aattactgat tctacactgg atgtattgaa
     9961 ataattagtt ttaggatcat agtcggttgg agtggcgatg atgacataat cagcatcccg
    10021 gtagtcttca tttttatcta atgtggcatt aaagtgtatt ttatctgatt gcaaaaactg
    10081 ctgaatttcc ttatcaacaa taggagatat ccgatcattc agcatagcaa cgcgtgacgg
    10141 taaaatatct aatgccacaa cctcatgatt ttgtgcgatt agaagcccgt ttgacaagcc
    10201 tacatagcca gtaccggaaa tggtgatttt catttcatgc tctcagaatt aacttaactg
    10261 tgaatcatga tgtttttagc atcgtgataa gatgctaaaa gttttaacgc tacgttgtca
    10321 gatggtcaac gcaacgaaaa acaattcaga ttttctttat aagaatagct gatgtttatt
    10381 aagttaatat taagcagtga aatttagtct ataagatatt tggcaaaaaa aagcccggtg
    10441 caatatacgc cgggcctcaa ttttattgtt ggttaaatca gattaatcca gccattcagt
    10501 atggaacaca ccttctttat caatgcgctt ataagtatgc gccccgaaat agtcacgctg
    10561 tgcctggatc aggttcgcag gcagaaccgc ggcacggtag ctatcgtaat aggcaaccgc
    10621 agcggcgaag gtcggaaccg ggataccgtt ctgtactgca taagcgacga catcgcgcag
    10681 cgcctgctgg tagtcatcgg caatttgctt gaagtacggt gccagcagca ggttagcgat
    10741 ctgcggattt tcggcataag catcggtgat tttctgcagg aactgcgcac ggatgatgca
    10801 gccagcacgg aaaatcttcg cgatttcgcc gtagttcaga tcccagttgt attcttcaga
    10861 cgcagcacgc agctgagaga agccctgagc gtaagaaacg attttgccca gatacagcgc
    10921 actgcgaact ttttcgatga actcagcctt gtcgcctgct gactgagctt gcggaccaga
    10981 gagaacttta gatgcggcaa cgcgctgatc tttcagagaa gagatataac gtgcaaacac
    11041 agactcggta atcagcgaca gcggttcgcc gagatccagc gcgctctggc tggtccattt
    11101 accggtgcct ttgtttgctg cttcatccag aatcacatca accaggtagt taccgtcttc
    11161 atcttttttg gtgaagatat ctttagtaat gtcgatcagg tagctgctaa gttcaccgtt
    11221 attccactcg gtgaaagtct gggccagttc ttcattagat agattcaggc cacctttgag
    11281 cagagaatac gcttcagcaa tgagctgcat atctccgtat tcaataccat tgtgaaccat
    11341 cttcacataa tggcctgcgc cgtcggcacc aatataagta acgcatggct cgccgtcttc
    11401 tgccacggca gcgattttag tcaggatagg cgcaaccagc tcataagcct ctttctgccc
    11461 gccaggcata atagaaggac ctttcagcgc gccctcttca ccgccggaaa caccggtacc
    11521 gatgaagtta aagccctctg ctgaaagctc acgattacga cgaatggtgt cctggaagaa
    11581 ggtattacca ccatcaatga tgatgtcacc tttatcgagg tatggtttca gggaatcaat
    11641 cgcagcatcc gtgcctgcac ctgctttcac cattaacagg atgcgacgag gcgtttccag
    11701 agattcaaca aactctttca ccgtatagta aggagccagt ttcttgcctg gattttcagc
    11761 aatcacttct tccgtctttt cgcgggagcg gttgaaaata gagacggtat aaccacgact
    11821 ttcgatgttg agcgcaaggt tgcgccccat cactgccata ccgactacgc cgatctgttg
    11881 ctttgacatt gtttactcct gtcttctata ttattaaccg tctattaacc gttatattat
    11941 taaccgtcgt gattttcatc ttctaatcaa ggaatacata tggttacagc atgcttctta
    12001 ctttatagcg ataagtatgc cattgggtgt atccataaga actttgaatt ttggatactt
    12061 ttctggatta aaattagcat agcttcgttt agatataaca ataaacgaac tattaaaaga
    12121 tttcatcttc tctattaaat catcctgtgg gattacctca cactcgggat tactaatagt
    12181 ccaatcacta tatcttgcta atccaaagac gtcaccaatt gccatccctt tatagaagta
    12241 aataccatcc tccaagccta attggataag attatctcca tataactcac tatattcact
    12301 tgctttcttc atcagttcat agctatagcg actttctaag cgtgaattaa aattcaagta
    12361 ttcagaataa aaatatttga attcaaaatg caacgatcgc cccgaaaaaa aaataataca
    12421 cacaaggata aaatatcgtc ccaacttgta atttgtaatg ttgatatctt ttattaaact
    12481 tgcaattgag atcgcaacca gcaatactga caatgggtaa atgggtgata aatacctgtc
    12541 accttgcgta acagaaaacc aaaataagac atatgttaaa acaatcgaga aaaataccca
    12601 aagtatttta ttattcctaa gtgtaaagac agacagtata gcataaaaaa caacatcact
    12661 gccaacttta ttaattgctt gttgcaaatc taatgtggca ggacttatac catgtcgagc
    12721 ttgttctgct gtttgaactt gtagatcaat ttcattccat aggtagtgac caaatatatt
    12781 tcctccagca ggactaaaag gatcaccaga gatccaaaaa ctacggacat accaataaat
    12841 gccaccaaga attgttgcca agctaaaaat aagaaatgtc tgtatgtttc tctttttcgc
    12901 tatcaatata acaaatatga tggctccata tatggcacca aatagtttta cgccacaagc
    12961 aattccaata aaaaaaccac ataaaaaaat taattcattt tgtgttttat tgttttctga
    13021 gcgaatatat ataagaatac tggctgcagc aaaacaaaga tagcgttaaa tcaatatatg
    13081 cataaccaag atgatcttta agtggattga tagtaaatag taggattgta gcaataatgc
    13141 taggataata agttttcgta atttcccatg aacagccaac aagtccacag gctattacaa
    13201 aaaaacagat atttgctaaa aactgagcta accttacatc tcctaactgt aaacctaaag
    13261 ccattaaaag atctgcattt tgcgggaaca gaggaaaacg caaatattgt tcaacaacaa
    13321 ttgcatggtg ctcaactaat gaacgagcca aaggtaagtg atacatagta tcatcccaat
    13381 caccgggagc tcgcagggac gataacaatg taaccacaaa aatcaaaaca agaaataaaa
    13441 taacaaacag cgtactgaac tcaatattat ttattgccag ccatctaaat tgctgacgtt
    13501 taataatgct taaaacccaa cacaccgtaa aaattaaaaa gatagcaatc ttattaaatt
    13561 gcccgaccat gcccagaaag taaattatca aaatatttaa agacatccct atgcataggc
    13621 aataaaaaaa taaagaaaat ttgttaagtc ctgccacccc ctccttaaaa aaagatgaca
    13681 atacaatatt accaatgaca aaaaatgtca ttccaaaagc aaaacaatac acaacataca
    13741 aatacaacgg caattcaatc atagtaaaat actctattta cattatcagt ttatagtttt
    13801 tttcagaaag ataaaataat gccttcattt cacgtctagt tgttgtattt gaatgcaaaa
    13861 taatgccaca tacaaatgcc attattgacg ataacatgat ggaggcagtt aaaaaggcag
    13921 tcggaaatct tctcaccaaa cctgtatcaa tataatcaac aatgatgggc aatcctaaaa
    13981 ttatagacaa agcagcaagt aaaacgccga taatactaaa aaagtatagt ggccgttcaa
    14041 gcgagtaaag tttaattatt gttttcagaa tcttgaaccc atcactccaa gttgatagtt
    14101 tgctcacact cccttcagat cgctcgccat attttgtatt aacctcacca tacttcatac
    14161 gtaattctaa agcatgaatt gtaagctcag tttcgatttc aaatcccctt gatagacatg
    14221 gaaaagattt aacatagcgt ctggaaaata cacgataccc cgacaacata tcagtaaaat
    14281 tccctttaaa gatagtctga acagtaccag tcaacagttt atttcctcag cgatgccctt
    14341 ttctgtatgt attttgatct ccattctctt gacgacatcc tacaaccata tccaattgat
    14401 tttttatgag atggttaatc atttctggag cagaacttgc atcataagtg ccatctccat
    14461 caaccattag ataaatatcc gcatctacat ctgagaacat acgtcttacg acattcccct
    14521 tgcctttcag aggaacagag tgaactgttg ctccagcatc ctctgccact tgtgctgtac
    14581 tgtcagtcga attattatca aatacatata tagaagcagt tggaatattc gttttgaaat
    14641 catttatgac ttttgctatt gccttctgct cattataaca aggaataatt acagcgatat
    14701 taaaagagtg atacataagc ccctcatata aactatttag ctaatgtgca agtaattgtg
    14761 tttttaacaa atcgtttact aacgaaaaat gaaatatttt gatgcgcaat atcctaaaac
    14821 caaactaaaa agagaaaagg tgaacaatgt aaatattgga gataaagcta agagttcaaa
    14881 taaaacgccg aataaataac tcatcgcccc cataaaaaat ataaatataa aatatctagc
    14941 cttggttgca gatactttaa atgagcatct tgcatttgca aaaaaagaaa aggttgctgc
    15001 acatataaaa ccaacaatgt tacttcggcc ttgaccaaaa gctaaattat tataacacgc
    15061 ataaaatacc ccccaatgta aagcagtatt gacaattcca attaaaaaat aactaaaaaa
    15121 agaactggtc aataacttcc ctatttttaa catcctttat tttgctccag aagtgaggtt
    15181 attactaatt tggatatttt ctatagaaaa tacccataaa actacaacgc ctataataaa
    15241 aattcccatg ggtgtaaaaa gaaatggatt agttatagaa acggtaatac tgaaaaaaag
    15301 tattaacgac atcattaatg atgtttttgt actatttaaa aaatagcctt tcaaaaagat
    15361 aagcaacata ggaactaatg atataaatac accaataacg cctgttttct gaagtatctc
    15421 aagaggtact atttcaatcc ttaatcgatc taatataaat gaaccaaatc ctcttccaaa
    15481 caagaacgtc gctaaatcaa catttttata ataaaaatat aaatcattaa atctaacaga
    15541 gtcagaatct tcactgcgtg atccgaccat gtaaagcata attataaata gaacgctgcc
    15601 caatattatt ataatagtat ttaaatatag cttatgtaat ttaaaactgg ctaataacag
    15661 tgataatgtt gtaaaaagta atacacctct tgattctgtc aataatatcg caagcactat
    15721 tatgagacat tttaaatatt ttttgtcgac aaaactgata atcgcaccga caccaaaaaa
    15781 gtagaaggat ttggaaaaaa aagccccatc gggacgaaaa aagaactcgc tagtcaatga
    15841 taagtgttca taaattaaat taaaatttaa caaaccgaaa ttcaacaaaa ttatgtatga
    15901 aatatataac agtgatccat ataatgcaaa tgcattaact attttaataa aaatctcaca
    15961 taaaaatact tttaaagtat atcttagtgt aaatagatat ataagtgaca gcataaaaag
    16021 gtatggttgc acatcaaaca gtatatcatt tattttatta ccagctaata agccgacaaa
    16081 gaagccataa aaaggaaata aaagcaacca tataaaaaga ctgacacatt tttgggtgat
    16141 tgaaaaattg aatgtaacta aattaaaaat taaaagtata aaactaaata caaataataa
    16201 atatcgcaaa gggaatattc ccaatggctc cagtaatctt ccaccaccac caataatcag
    16261 ttcaatacat aaaaatgtta taaaaatttt atttatatta ttcatattcg taaggtgatg
    16321 ttttttaaaa tatgtttaaa atcaacatta ataaaatata aattttatca gcctttttcc
    16381 gccttaactt ccaaaaactt aaagccaaga tcaaattgcc tatataacct ttacccaatt
    16441 gattattcac aaagctatca tcagctaagg cattagctat tttagttact tcttttcgat
    16501 accacttgtt tctcaccaaa ccaagccgtt tgtattttgc tttaaaacta atgtttgctc
    16561 ccacttggtt gttttcatgc tgtctgtaca gcattgttgg ttttctatca atataccacg
    16621 aataatttct cgtccgcgca aatgaatata aaaaccaatc atgcagacag atatcttgtg
    16681 cagcattttt attatttatg atgaacttct taaattcaat aagcgtttct ttttttataa
    16741 catatgtaca ccccggacct gctgcttcaa aaaaataatc gaattgagtt tgcgactgag
    16801 ccttatcaac taaatttttt tgccctgatg gataatatgc aattacattg ctggaataac
    16861 aattcgcatt attaaatacc aagcagtcaa ttgcattttt tattttatct ttataccaaa
    16921 tatcgtcctg atcactaaaa cagatataat catagttttc aagatcgaca tctttaatta
    16981 atctataaaa atttttacct gcccccccaa acttattgac gcttggaagt atatttataa
    17041 ttgagggata tgataattga aattcttcac aaatatttaa tgtattatca gttgataaat
    17101 catctgatat aaaaattgag atgtctacat ctttttgttc aataatactt tttaattgtt
    17161 cctcaatcca gcattcgcca ttatacgtag ccataagtac agctactctt ttttgatcag
    17221 aactatgcat tataacgacc gcccccagta attcctctta ttccatccag cgtagccaaa
    17281 atatgttttt tcatatattc aattttattt ttttgagttg cgataattaa agtcgtatga
    17341 attaaagaaa aaacaacttc cctaatagag agtaaaagag gcacatgatt ttttctaagc
    17401 aaaagaaatg catttcttac tcgataataa cgacgtactg gcgagtgaat tggtatgtta
    17461 atcccacaga tttttactga cttatcccca atagcatgaa ccatttctat atcaggaatg
    17521 atatgaacta atatgccata gtttaagcat ctaagacacc attctgtatc gacataatca
    17581 ataaaaagtt cctcatccat cattccaaca atctcaaaaa cttcttttga aaccatggtt
    17641 ccggatgaga ttgttactga acttttaaac ggtgtctgtc cttcagtgac atgaattttc
    17701 tctcgaagac catttttttt gatattacaa ataggataat aatgtgactt atctctatca
    17761 ataaaaactg ggccagctat ttttgcattt tcattatcca tatagcagga aagttttttg
    17821 ataaactcat tgcgaatacg gctatcttga tcaaaaaaaa ttatcttctc tgcccctttt
    17881 tcaagagaat atcgaatccc aatattttgt gcttttgcta taccttcatt cctgccaaga
    17941 caaattaaat gaactttttc aatgtctgct aatttatctt tcaaggtgaa attagtccta
    18001 ttatcgacta ctacaacgta acaattttgc tcctttagtt cagtgatcag ttcattcaga
    18061 tttttaagtt cgggattata tgtcacaatg acagcgtaaa tattactatt cattatccaa
    18121 gtgactcagt aattggttaa ttttagtttt catatacaga acacattcta gtataaagcc
    18181 attgtaataa cagagcatct aaaattacac gtatgctcca ggatattgcg gcacccagta
    18241 acccatattt ttcgaccata aaatacaaaa gggctaaata aggaaccaat tcagcacaat
    18301 gaatcaaagc agtaatttta gattttccta atgcctggat tgcagagaat ggtatcattg
    18361 ccagcgcatt aaagaaaaaa ccgatcaata acacatttaa tatattaaag gaattaattt
    18421 ggtaattctc accaaaccat attttcaata caagccctga atataacaag cctatgatta
    18481 tcaccggcaa acagattaga aacattaaaa gcaatgattt atttacatta cgtttaaatt
    18541 ctttaaagtc tttgatgtta cttaaccttg gaaacactgc cctcccgatt gctgcaggta
    18601 ttattcctaa ttttagaata acctccgctg gtgcagaata aaatgcaact ttatctgccc
    18661 ccataatatt tgacactata aatcgatcaa aatacaccat gaccggactt atgatattac
    18721 ttactgtcat ccaaccgcca aagaaaaaga gtcgtttaaa ggttttataa caaaaatgaa
    18781 cccccgaaat tttaatatca ttcctgacat aataggcaga gactaaaatc gaaataactc
    18841 ttgcaaatat taaaccagca accgctgccg acaatgtagc actataaaaa acaaatatgg
    18901 ccggaattcc cgcgatgcaa gagcttgata tagatttttg aatatttaca atgccaaatt
    18961 tttcatcccc ctcaagaatg gctgaccata attgatttag aataaataat ggaatgcaaa
    19021 tagccaacag tttaaatgct agttgtatat cactatgttc aacaccagaa atttttagat
    19081 aattaacaat tccatcagag aaaatcaata ataaaaaagc accaaagcat gaaaatagca
    19141 ccaaaaaaga tgtacttgtt gaaattacct tggttctttc atgatgatta tcacgatgaa
    19201 ttgcaatttc tctaataaca gagcgagtca ggcctacatc aaaaattcca gcatacccaa
    19261 caagtgcaat tgcaattgta taaacaccga aaccttcagg ccccaaacct cgagcgagaa
    19321 atcctagcga ggggatagca attaaagttg gtattgcata gccaaaaagg ttccagacac
    19381 tattttttat tatgctcatt attacatttc tttatcatat aaaataatca gcatctacaa
    19441 atttttgacc attaatatct ttatcagaaa gcaatggatt ttgaaccggc cattctatat
    19501 ttatactttt gtcgctccat aaaatcccac gctcttgcat ttttgaatag aaattatttg
    19561 ttttgtagac aaattctgct tcatcactaa gaacgcaaaa cccatgagcg aatccttccg
    19621 gtatccataa ttgacgctta ttctccgccg acaaatttac accaacccac ttgccaaatg
    19681 ttggtgatga tttccgaata tcaacagcta cgtcaaaaac ttcaccgaca acacagcgta
    19741 ccagttttcc ttgagcataa ggttccagct gatagtgcag tccgcgtaac acacccttag
    19801 ttgatttaga atggttatcc tgaacaaact caacctttcg tcctacagcc tcttcgaaaa
    19861 ctttctgatt gaagctttcc ataaaaaagc cccgctcatc accaaatact tttggttcaa
    19921 aaattagcac atcaggaatt tcagttttaa ttacgttcat tttattaata acctttaatc
    19981 attttcagca gatactgacc ataagcattt ttcttcagcg gctcggctaa taccttcact
    20041 tgctcagcat caataaagcc tttacggtaa gcaatttctt ccgggcagga aactttcagc
    20101 ccctgacgct cttcaattgt tgcaatgaag ttgcttgctt caataagact ttgatgtgtc
    20161 cccgtgtcca gccatgcata accacgcccc atcatagcga cagacaaacg tccttggtcc
    20221 atataaatac ggttaatatc ggtaatttcc agttcgccac gtgcagatgg tttaaggttt
    20281 ttcgccattt ctacaacgtc attgtcatag aaataaagcc cagtaaccgc atagttactt
    20341 tttggttcca gcggtttttc ttccagacta attgccgtac cgttatcatc aaattccacc
    20401 acaccgtagc gctcaggatc atttacgtga taggcaaaaa ccgttgcgcc actctctctg
    20461 ttaacagcgg tatccattaa tttcggcagg tcgtgaccat agaagatatt atcaccaagt
    20521 accagcgcac aatcatcacc acctataaat tcttcaccaa tgataaatgc ttgcgccaga
    20581 ccatccggac tcggttgtac tttatattgc agattaagcc cccactggct tccatcacca
    20641 agcaattgtt gaaaacgtgg tgtatcctgc ggcgtactga taatcagaat atctcgaata
    20701 cccgccaaca taagcgttga aagcggataa taaatcatcg gtttatcata aatcggtagc
    20761 agttgtttgc tcacagccat tgtcacagga taaagacgag tgccggaacc accagccaga
    20821 ataatacctt tacgcgtttt catttcacta ttccttttaa ttcatctcgc tctggcatca
    20881 tgagcgagat gcaataattt gttaaattgc cgtggtcgta aataattcgt tgagcatacg
    20941 tttcacgccc acctgccagt caggcaagac aagcgcaaag ttctgctgaa atttttcggt
    21001 attgaggcga gaattatggg gacgacgtgc tggtgtggga taggccgttg tcggcacggc
    21061 attaagtttg ttaagagcaa ggttgatccc cgctctgcgc gcttcttcaa ataccagagc
    21121 ggcataatcg tgccaggttg ttgtaccacc agcaaccaga tggtacaaac cagcaacttc
    21181 tggtttattt gcggccacgc gaatggcatg agcggtgcaa tcagccagta attcagcccc
    21241 cgttggtgcg ccgaattgat cgtttattac agccaactct tcgcgttctt ttgccaggcg
    21301 caacatcgtt ttggcaaagt tatttccttt accagcgtat acccagctgg tacggaaaat
    21361 aagatgcttt gcgcaatgtt cttgtaacgc tttttcccca gctagcttgg tttcaccgta
    21421 gacatttagc ggtgcggttg catccgtttc cagccatggc gtgtcaccgt tgcctgggaa
    21481 aacataatca gtggagtagt gtataaccca ggctccgact tcattagctg cttttgcaat
    21541 cgcttcgacg catgtcgcgt taagtaattg cgcgaaattc ggttctgatt ccgctttgtc
    21601 tactgcagtg tgagcagcag cattaacaat aacatcgggg cgaatttttt tgacggtttc
    21661 agccacacct tcggggttgc tgaaatctcc acaataatca gtggagtgaa catcgagcgc
    21721 aatcaagtta cccagaggag ccagagcacg ctgcagttcc caacccacct gccctgtttt
    21781 gccgaaaagc aggatattca ttactgacgg ccctcatagt tttgttcaat ccacgactga
    21841 taagcaccgc ttttcacatt ctcaacccaa tttgtattag ccagatacca ttccaccgtt
    21901 ttacgaatcc cgctctcaaa cgtttcttgc ggtttccagc ccaattcgcg gctaatttta
    21961 tcggcatcaa tggcgtaacg gcggtcatgc cccgggcgat cggcaacata agtaatttgc
    22021 tcacgataag atttctcttt cggtacaatc tcatccaaca aatcacaaat agtgagcact
    22081 acgtcgatgt ttttcttttc gttatgtcca ccaatgttat aagtttcacc cgctttacct
    22141 tcggttacga cggtatataa cgcacgagca tgatcctcta catacaacca gtcgcgaatt
    22201 tgatcccctt taccataaat aggtaatgcc ttaccttcca gagcattcag aataaccaat
    22261 ggaataagtt tttccgggaa gtgataagga ccataattgt tcgagcaatt agtcacaatg
    22321 gttggtaaac cataagtccg cttccaggca cgtaccaaat gatcgctgga agctttagac
    22381 gcagaatacg ggctacttgg cgcgtaagct gtcgtttccg taaatagcgg taacgcttcg
    22441 ttattatttg cttcatccgg atggggtaag tcaccataaa cttcatcagt agaaatatga
    22501 tgaaagcgga aacttttttt cttttcatca ttcagagcag accagtaatt gcgcgccgct
    22561 tctaaaagaa cataagtccc cacaatattg gtttcaataa atgccgcagg gcccgttatt
    22621 gaacggtcaa cgtggctctc tgccgccagg tgcatcaccg cgtctggctg gtgctgtgcg
    22681 aaaatacgcg acatggctac agcatcgcag atatctgcat gctcaaaagc ataacgttca
    22741 gaatcagaaa catcagcgag tgattctagg ttcccggcgt atgttaattt atcgacatta
    22801 acaacactat cttgcgtatt atttattatg tgacgaacaa cagcagaacc aataaaccct
    22861 gcgccaccag taacaagtat cttcactttt ttattccata tagctataga gcatgctgtg
    22921 aaatagactg ctccagattt gattaataga ggtattaata cacgctaccg cccctggctt
    22981 aacagctacc agtgcactgc atgcatctca acgatgtggc gagcgttagc cactcgcgcc
    23041 aaatccgaaa aattcaaacg ttaattgtct taccaaacag ccgtagaaac aaggaaaatc
    23101 ctggaaaaat ttgaataata tgagctatgt aacttcttgt ttttataggc tttataaaaa
    23161 cggcaacggc aggaaaattc aatgagcaaa acttatgcca cgaatcttca ctgccgttat
    23221 aattttctta tcaaccgtta catccggtca gattttcatt attcgcttaa cagcttctca
    23281 atacctttac ggaacttcgc cccttctttc aggttgcgta ggccatactt cacaaacgcc
    23341 tgcatatagc ccattttttt gccgcagtcg taactgtcgc cggtcatcag cattgcatca
    23401 acggattgtt ttttcgccag ctcggcaata gcatcagtca gctgaatacg tccccatgca
    23461 ccaggctgag tacgttccag ttccggccaa atatcggcag aaagcacata gcgacctacg
    23521 gccatgatgt ctgagtccag cgtctgcggc tgatccggtt tttcgataaa ttcaacaata
    23581 cggctgactt taccctcacg gtccagcggc tctttagtct ggatgacgga gtattcagag
    23641 aggtcacccg gcatacgttt tgccagcacc tggctgcggc ccgtttcgtt gaaacgtgca
    23701 atcatggcag caaggttgta acgtagcggg tcggcgctgg catcgtcgat cacaacgtct
    23761 ggcagtacca cgacaaatgg gttgtcacca atggcaggtc gcgcacacaa aatggagtgg
    23821 cctaaaccta aaggttcgcc ctgacgcacg ttcataatgg tcacgcccgg cggacagatg
    23881 gactgtactt ccgccagcag ttgacgcttc acgcgctgct caaggagtga ttctaactca
    23941 taagaggtgt cgaagtggtt ttcgaccgcg ttcttggacg cgtgagttac caggaggatt
    24001 tctttgatcc ctgcagccac aatctcgtca acaatgtact gaatcattgg tttgtcgacg
    24061 attggtagca tctctttggg tatcgcctta gtggcaggca acatatgcat cccgagaccc
    24121 gctacaggaa taactgcttt taaattcgtc attatttcat ccacctgtaa aatggttgct
    24181 gaattatagc ttgttcgatt tttttcgcca gcatcaatta ccctgaattg attactgaat
    24241 tacttgtgat gttacgccgc ttcgttgtgg attgcagtag cattgttcct aagtatggct
    24301 ccatttttcc aggaatggtc gcaaatctac tccctccgtt ccggcaatct aaagttaatc
    24361 ttttccacat taacaatatg gtgattaatc ctgtcgatat cgacggagct ttgccctctt
    24421 tcattcaccg catgaacatt tgcaagagac agcagtgttt cttttttcgc cataaagacg
    24481 ccgcgaacgt ctttgcgcat gtcgaagttc atgctcaatg ctggtccaac tgaggattcc
    24541 tgcatcacat tgatatttcg tataaaaaga tgttgcggct tgttgtgtaa ctccagcgag
    24601 gcacgcttca tctcaatgtt agttagcgac acaaaggaga cggcattccc ggcggagatt
    24661 tggatgccgc gcaatttata agcaagatgg gtattatcca gttgaatatt attcactcgg
    24721 aaattttgcg gtatcgagag atatttgcct ttaattaccc catagccgat taacatcccg
    24781 gcgctattaa tcatttcaat attatcaatc acgaaattgt cacaaccgta aatagcgact
    24841 gtcgcgttat caatgcccgc tttcttactg aaatccggcg tgatattgcg ggcattgata
    24901 ttacgaataa caaaatgttt accattttca acatggttca actgccgaca atccgatccc
    24961 gtgatattcg ccacgacaaa gtttttcact gcctggtctt ccgggtagtt gttatcataa
    25021 gtgcttcccg caaggcctat gccgatgccc cagttgattt tgccgttggt acagttgata
    25081 cgctcgatga catggtcaga tatcaaaata tcactgtcgt taattgccac gttccattca
    25141 atggcgtcgc cctgtaagtc gctgaactta cagttggtaa tgttggcacc gataatctgg
    25201 ttatgaaatc cctggcgtaa gatggcgtaa ttagcgtggc tgacagtgag gttatcgatg
    25261 gtcaggttgc gcatgacccg tttgtttttg ccgccaatat aaatctgcat taccgggcca
    25321 aagccgctca tcgccagccc tttgatggtg cagtcagaac cacgcacatc cagggtaatg
    25381 ttatgcatac cgccgccctc tccccctgtc acctggctgc cgtcctgtaa gacaaatcgc
    25441 cctctgccgt tgccgcgcag gcttccaagg atgtgtaacg ttttgccggg agggatgaag
    25501 atgccggtgt tgatattgtc acaaacgaat ccggcaggca cgacgaccgt ttgcccttcg
    25561 ctgaaggctt gtttaaatga ggcgatccag tcatgtgggt tgtagtcgtt aatgttaacg
    25621 ctttgtcggg cgggaagtgc gcgagcgaaa ggggtatgga ggaaggcaag cgccgagctt
    25681 gccgtcagga aggtgcgtcg ggagagtgtt ttaaatggca tacgttctcc tctataaagc
    25741 ctgcagcaag ctggcgagtt ctcgattaat cacctgctgg ttaaaatcgt gttcgacttt
    25801 ttcgcgcgca cgtttgacga ccggagccag ttcgtcggta tccagttggc taaacgccgc
    25861 cagacgttgt gccagtgcgc gagcatcgtt ctcaggcacc agccagccgg atttatcggc
    25921 ctccaccagt tcaggaattc cgctatgcag agtagaaacc accggaatac cgaccgccat
    25981 cgcttccatt agcgccaccg gaatgccttc catatcgcca tccgcgcccg ttaccgatgg
    26041 caacaggaag acatccgcgt cgtcgagcat ctccttcact tcatagctcg gtttaaagcc
    26101 cggcatctct accacatctt ccagttgata ttgttcgatg agggtgcgca ggcgtcgttc
    26161 ccacaggcca atgccgagga tgcgatagcg aaatgccacg ccctgctctt tcaactgacg
    26221 gcaggcttca atcgccacat gcaggccttt tttctcggtt aagcgtgcga cagatatgat
    26281 ttccagcggc gttgcgggcg ctttcaccgg acgcgggcta aagcgcgtca tgtccacgcc
    26341 catacgcgat acggcgattt tttctctggg gcagcccatt ttttgcagcc ttccggccca
    26401 cagatcgctt atcggtaaca tcaggtcgcc acggcgaaac agttgttgat attcgggagt
    26461 gtagtggttg agcacttccc gactggagat atcaataccg tggaagatgg tggcaatttt
    26521 gccgcgaatg atacccagtt cgcgtagttt tgctgcggtt acccccgccg ggccaaaatg
    26581 agcgatgaac acatcggcgc gaaacggtgt tgcgacctga ccacaaatgg cagacaaaat
    26641 caggttccgc gactcggcac catagcgttt gaggttgagc gcatgccagg tatttttacg
    26701 atgaatgcca cgtaaggtct ggctggcgcg gtggcgcagt ttcgctactt tgcctgcagg
    26761 ttcgtcctgt aaccagcggg ttctggcgac aaggttgtat ttcgtccatg ccgcgtgggt
    26821 gttctctgta tcgccttttt gcaaagcgac aatctccact tcaaagccca tatcaataaa
    26881 cgcggtaatt tgattgagga cgaaggtttc cgacgacagc gggaatttca ataagaagaa
    26941 gccgaccttc atttcacctc cccgatgcgc tcaagcacag actgcaccat ctgcattccc
    27001 gtctgacgtt cgcgactgac ggcttcgtta aatcgagcgt tcagcaccgg aagctggcct
    27061 aaggtatccg caatcatcgc ttgcaggctg ccgtccagta aatgacggat atcaatcgcc
    27121 atctctggca gtcccagctg ctgcataatc ccggcggatt tatgttcata gttgatggca
    27181 atcgccggag tagcaaagtt cattgagata atggcggagt gcaggcgcgt accgacggtg
    27241 agttcacagg ctccgagaat tttgcccatt tccagatcgt tgagttcatc catcactacg
    27301 tggtaacggg caggatcgct gatgtgctgg cgcagattga gcgccaccat gcggtcgtct
    27361 ttgttatagc tgtcgatacc cgtacaggtg gagagcgcaa tcacctgata gccttcatcg
    27421 agaatgcgat tgaccacccc ggcaaaggct ttttcatacg cttgttgagt ggtgccgaga
    27481 cgtttgtcga acggtgccag ttcgcgcagg gtaattgcca ctgttttctg ttgtgcggca
    27541 acgtccagcc agtgttgaac ggcatagctg gcggtgaagt cttcggtgtg gtgatcgacc
    27601 agccacgcgg tatcgacgcc atgttccact tttgcggtgg tgatattgct gcgtttcatc
    27661 aaatcaaggc tgaccgattc gcgcaggatc agcgcgtcgc agtgaccaaa gacatagttc
    27721 gccagttggt taaattgctc atcctggaag gggccgacgc tgtgaccaat cataaacagc
    27781 ggctttttcg ccataaatgt gcaaagcgca tgttcaaact gcggcacgcc gtagagatcg
    27841 acaaaaaacg atccgccgac ctgaataatg gcgtcgtaac ctgacagcag gcgcacgaag
    27901 tcggtgaatc cctgggcgat ggcgatattg cgcagcttgc cagtgtcggt gacgcgtgag
    27961 agcaatacct gatgctggta gctgcgacgg aggacttttt taacgcgccc gacaacgccc
    28021 gccgcgctat tgtgttgtgt catctgcagg aacagcggat cgcccattac cgggcggttg
    28081 agcagccagg aagaactgac cggatagcgg ctcatcacgt ccacttcggt atgtggattg
    28141 aggatgttga tggcatcaag taagccgcgc aagatggcgc tgtcgccacg attgccgcaa
    28201 gtgtggttgc ccagaataag taatttcatt ttttcctcat aaatttgatg ccaggtgagg
    28261 ctgtgtttgt tgtgttcgtt gccgtttttt gcctgatgcg acgctggcgc gtcttatcag
    28321 gcctacaggt cccgagcaca gaaccgtagg acggataagg cgttttacgc cgcatccggc
    28381 aaccgttgtc ggaaccgaaa acagcaattc accccgcccg taaaagcatt ttcatttttt
    28441 cgctgcgaca aaactgacgc ttcacttcca ccaccagcgg atggcgtgac agcacaatca
    28501 tcaccacaaa cgccagcacg cccgcggcta tttgcaccgc cagcagcatc cccagcgcca
    28561 gttgcccttt cagcacaatg cccagcgcat aactgaccac cagcgtcggc aacgagagat
    28621 aaaacggcag ccacaaactc aggatgtact ggcgataact ggaaccaaga accggtttaa
    28681 tcatcacgaa gtaactcaga atggtgttga taatctgcac cagcaggaaa ccgagcgtga
    28741 cgccgattgc gcccgccatc tgcccaccaa taacaatcgc cggaataaac agaaatgttt
    28801 tgaatacatt gaatttaaag ctgatatcga cccgcgcttt cgccatcagc agcgaaccaa
    28861 tcgggttacc cacggagcgc agcagaccca caatacacag caattgcagc accggaataa
    28921 tgctgttcca cttttcgccg aataccagcg gtacgaaatt attcgacacc accatcagcc
    28981 ccagcagcgc cggaaagttg ataatcccca ctatcgacag cagcttgtag aagttaacac
    29041 gcagcttttc ggtgtcgtcc tgaattttgg caaacgccgg aaacaacacg cgggtgatga
    29101 ttgggttcag cttcatcggc ggtacgacgg ccacgttgta cgccaggttg tatccccctg
    29161 ccacgcccgc gccaagaata cgcgccagca ccagcgttga aaggttggtg ttgagatagt
    29221 tgatgatgct gtccgccgtc agccaggcac caaaacgtaa gttcggtgcc acagacgcca
    29281 gcgagaagtg cagaccgggg caataaattt tgcgtccaaa gtagccaaac agcagtgttc
    29341 tcaccgcact attgaccaga taaccgagga tcgcggtcat cgccagcggc cagaaatggg
    29401 cgctaactac cgtaaaagtg aagcccgcca gcaccgcgct ggtttcgatc atgccgattt
    29461 tgttgaactc cagctccttc tgcatcaacg cgcggaactg ttgcccgtgg gggatcacca
    29521 caaacgccag cgacaatgtt ttaatcaacg gtgccaggtc cgggttattc agcacgtcgc
    29581 cgatgacatc actcaacaaa aacaccgaca cacataccac aagccccagc ccgacgttca
    29641 gccagtacag cgtggtgagt tcaaggtgac tgatttcttt tcgctgaata atcgagttag
    29701 cgataccgaa gtcagaaagc gtatctgcca gcgcgataat caccagcgac acagtcagca
    29761 ggccgaactg gtggttgtcg ataatccgcg ccagcacggt catctgcacc agcccgaggc
    29821 cgatgatgat caccgtggca atagccgacc acttcgcgcc gctgatggtt ttttcacgta
    29881 agctcatatc aatatgccgc tttgttaacg aagcccttga ataccgtcag gaaaacgatt
    29941 ttgatatcga accagacgct ccattcgcgg atgtactcaa ggtcgaactc gacgcgtttt
    30001 tccattttct ccagcgtgtc ggtttcgccg cgccagccat taatctgcgc ccagccggta
    30061 atgcccggtt tcactttatg gcgcagcatg tagccttcaa tgagctggcg atactgttcg
    30121 ttatgcgcta ctgcgtgcgg acgtggaccg acaatcgaca tccccccggt cagcacattg
    30181 ataaactgcg gcaattcgtc cagcgaggtg cggcgcagaa agttccccac tttagtgacg
    30241 cgcggatcgt tctgcgtcgc ctgggtcacc actttgtcgt tctccatcac tttcatggaa
    30301 cggaacttcc acactttgat cggcttgcca tccataccat agcgagtctg gcggaaaata
    30361 accggccctg gtgaactgag tttcaccgcc agcgcaatac agcacagcac cggggagatc
    30421 agcagcagaa taagcgtcgc cagcacaatg tcttccgcac gttttagcag gcggttaccc
    30481 cccgaaagcg gcgtgtcata cagcggcacc accggaacgc cgttcatctc ttcgagacgt
    30541 gaatggagaa tgttgaaggt aaagacgtcg gggatcagca gcaccgaaca ggtggtatcc
    30601 gccagttgat gggccagttt tttcactcgt gcgccgtcgc acatttgcat cgcgatatag
    30661 acgttatgaa tcttgcccgc ttttgcatcc tcgaccagct gttgcaggtt acccgcccag
    30721 tcgttagaaa cgccgcccgg tttcgtgtcg tggtaaacgc ccaccacttc aaaccctaac
    30781 cacggctgat tacggaaact ctccatcagc atttgtccgg cggctaaatc ccccgctacg
    30841 gcgaccatcc gcttgttgta gccatgatta cgcagccagc ccgccccaat gcgaatacat
    30901 gaacggcaaa ccaccagtcc gatgctggtc agcccatacc acgccagcca gattttcagt
    30961 tgcgtgtcga aatcattgtt gaacgccacc agtccggcgc tgaaaatcac gcttaaggtc
    31021 cagttttgta gcaacagggc aaattctgtc gctgcccgaa caccgcgcca tgagcgataa
    31081 aaatcggtga tgccgcccag catctggaac accaccagcg taatcagcgc caccaacagg
    31141 tgcatgtaga ggaatgacag tccgctgact tcgcaaacca gccatagccc ggcaaacatg
    31201 atggtgatat ctgaaaagcg ttgcaccata gagattaacg atgcattggt tttcgctcgc
    31261 tcgcgctttt ttagatttgt catcgttgtt cctgttatta gccccttacc cgcagtgggg
    31321 taagggaaga tccgacatta ctcgttcagc aacgtcagca gagttcgcgt tcgcgcttcc
    31381 atcagcggca catcaccgcg cgattccaca ttcaggcgca ccaccggttc ggtattggag
    31441 gtgcgcaggt taaagcgcca gtcggcaaag gtcatgctga tgccatcgct gcgatccacc
    31501 gccagcgcct cacggctaaa atgctgttcc acgcggttaa tcgcctcaac gggttgcgcc
    31561 agtttgctgt tgatctcacc gcttgccgga aacgccgcca tccggtcgcg caccagttcg
    31621 cccagcgttt ttcctttcag gcacaccagt tcggcgacca gcagccacgg gatcatgccg
    31681 ctgtcgcagt aagcgaaatc acggaagtaa tggtgggcgc tcatttcgcc accgtagatg
    31741 gcgtcttcct tgcgcatccg ttctttaata aaggcgtgtc cggttttcga cattaccggc
    31801 gtgccgcctg cagcagtcac cacatcaata gtgttccagg agagacgcgg atcatggatg
    31861 atcttcgcgc cgggattttt ttcgaggaac gcttctgcca gcaggccgac aatgtagtag
    31921 ccctcgataa actgcccttt ttcgtcaaac agaaaacagc ggtcaaaatc gccatcaaag
    31981 gcaatgccca tatccgcgcc gtgtttgatg accgcattac gggtgtcgtc gcgacattcc
    32041 ggcagcagcg ggttaggaat accgttgggg aaattgccgt ccggtgtgtt gtgtactttg
    32101 attaattcca cgggcgcgcc gagggcttta aagcgggctt caatggcgtc caccaccgga
    32161 cccgctgcgc cattcccgga gttgatcacc agcttgagcg gcgtgagatt tttgacattg
    32221 atataaccga acaggtgatc aacgtaagcg tcacgcaggt tgatttgctg atagcgaccg
    32281 cgtttggttt catcgacggg aggaaagtcg ttggcttcag ccagacgctg gacgtcgcgc
    32341 agtccggtat caccgctgat cgggcgagcc ccctcgcgca ccagcttcat gccgttgtaa
    32401 tccatcggat tatggctggc agtaacttca atgccgccat ccacgccgag atggaacgtg
    32461 gcgaaataga tctcttcggt gccggacata ccgatatcca gcacatcgac gcccgcatcc
    32521 tgtaaacctt ttgccagcgc cagttttaag gtttcgctgg tgagacggac atcaccgcct
    32581 aacacaatgg ttttcggttt gagaaattcg ccataggcgc gaccaatgcg ccaggcgata
    32641 tcttcattca gttcttcgcc taatttcccg cgaatatcat aggctttaaa gcaggttaat
    32701 tttttcatgt cgttaccctt tttcaggcaa tagttggccc tgaccgaaac ggccaatttt
    32761 atttttgtcg ttatttacac cgcggtttcg cattcattgc ctgatgcgac gtttacaccc
    32821 gtccgtagcg atccgcgaag cgcaccacat catcctcttc gagataagag ccggagcgca
    32881 cttcaattaa atcgagcgga attttccccg ggttttccag gcagtgcgtc gcccccagcg
    32941 gaatataaat ggactcgttt tcaccaagca gtttgatatc accatcaatg gtgacttttg
    33001 ccgttcccgc gaccactacc cagtgttccg cgcggtgatg gtgcatctgt accgacaaac
    33061 cctcgcccgg tttcacggtg atgcgtttca cctggtagcg gtcgcccgcg tcgatagagt
    33121 catatttgcc ccacggacga tacacttcgc gatgcacccg atgctcatgg tgaccatcgg
    33181 ctttgatctg ctcgaccact tttttcacat cctgtaccgc gttacggtcg gcaatcagca
    33241 ccgcatcttt ggtctgcacc actaccagat ctttcacccc gacggtggtg actaggcctg
    33301 attcggcata cacatagctg ttttcagttt tgtgattaat cacatcgccg tggcaaacgt
    33361 tgccctcggc ggtgtgggcg ctgatctccc ataatgaaga ccaggaacca acatcgctcc
    33421 agcccgcatc catcggcacc acaacagcat ctgccgtacg ttccatgacc gcgtaatcca
    33481 ccgactcttc cgggcaggcg agaaacgctt cttcatccac acgaataaaa tcgagatccg
    33541 gatcgacggc gctcatcgct ttttcgcagg catcgaggat atccgggcga tattttttca
    33601 gttcttcgag atagcgtccg gcgcggaaca ggaacatgcc gctgttccag taatattcgc
    33661 tgcttgctac ataggcctgg gcggtttcca gattcggttt ttcgacaaac tgcgccactt
    33721 caaaggccac cgtatcctgc tcacccgcag acacttcacc gcgacgaata tagccataac
    33781 cggtttctgg tagatccggc acaatgccaa aggtcaccag cttgcccgct tcggcataag
    33841 gcatggcatt acgcacggcg gcacggaacg cgtctttatc ggcaatcaca tgatccgccg
    33901 ccaataccag cattaacggg tcgctctccg ggctatgacg ttttgccgcc agcgccgcca
    33961 gtgcaatggc aggtgcagtg ttacgccctg ccggttcgag aataatgttc tcggtgagtt
    34021 tgttcagttg acgcagctgt tccgcgacaa taaagcggtg ctgctcattg caaatcacca
    34081 ccgggctttc gcactccacg ccgttcaggc ggcagatggt ggtttgcagc atggtgagat
    34141 cgcctttcag gcataaaaac tgcttggggt aaagtacgcg ggaaagcggc cataaacggc
    34201 taccggagcc acctgccatc acaactggat agagtttcga ctgcgccata attatccccg
    34261 aatatcattt ataaattgac gtaacacgtt ctctttatcg agcgtgcgtt cggcatattc
    34321 acgtgccacc gtgttatgtt tgggcagcag gagcgcctga cgaatccccg ccaccagcgc
    34381 ctcgaccgat tccggttcta cgcaaaccgc aatacccgga aaggtttcgc aaagctgccc
    34441 cagttctgtg tgggcctctg cggtaatcac cgcgttaccg cctaccgcca gaatattggt
    34501 cagtttcgac ggcaatacgg catctgccgc gccgcgtttt tgcaccacca gatggcaatc
    34561 gcccatcttc agcagtgcgg gtaaagcgtc atacgattgc agcgggaaaa attgcatgtt
    34621 gcgcagtcca cgctgctgcg ccattttttc cagccgcgct ttgccgccgc cctgcccgac
    34681 aatggcaaaa agcagcggtt catcgcgcag gagatcggca gcttcaataa cgttttccag
    34741 cccctgcttt tcaccaatat tgccggagta aagaatgatt tttttgttat ccggcaggcc
    34801 aagctggtta cgaagggcat caacatcggc gtctgcaaca tgctgaaaac gggcaatttc
    34861 cgaccagttg gggaagaaga tgacgttatc cgccgccacg cctttttcga tggctttatt
    34921 catcatcgaa cgcgaaatcg tggaaacgtt atcgacgtta tgcagtccgc tacgttcgaa
    34981 cgccgttgcc agctgtgcca ctttgccgcc tttgcctttt ccggcaaggc ccagccccag
    35041 catggcgtcc acttcgtaat cctgaatatg cagcacggta cgcgcaccag agagcttcgc
    35101 cagcaggcgc attcccggcg tgcaaaacag cgttggcact acgccaataa tgcgatccgg
    35161 cttccagcga cgttgcgcca tcagtggaaa gaaactgctg acggcaaaac tgccgagatg
    35221 caacaagcgt ttcagggtgc tcggctgttt tggcacgtac agcgggcagc gccacaccgt
    35281 ggcggcccct tcttctcgtt tgtagcgcca ggcggaatag ttctcaccca cctgccattg
    35341 cgggtagtaa ggcggtgcgg taatgacccg cacctcatga ccttgtgccg ccaaccattc
    35401 caccatctcg ccggtgtatt tgccgatgcc ggttaactcc ggcgagtagt taatgccgta
    35461 gaccagtatt ttcataatcc gggtactccg gcacgcttct cagcgagaaa ataggcgcgg
    35521 ctgttagcgt gaacatcgtt actggcgagc agcgcgtccg gcgtcagcca gcggtaatcg
    35581 tcatgctgct catccggcag taacagctct tcttccgcta ctctgaagcg aaaaccgagc
    35641 accacatagt gagtggtgaa atccgtgccg gagaagttat cgtcataaaa atgctgccag
    35701 acaccgtaaa actggcctgc tgttatcggc agacgcagcc ccagttccgc catcgtcagc
    35761 cgctcaaatg cggcttccag cgtttcgtct ttctgcacgc gccctcccgg cacaaaccag
    35821 taaccctgcg ccgggcggtt ggttcttttg ccaagcagaa actcgccgcg actgttctcg
    35881 acaataaagt cgagagagac aagcggtgtg gagcgcacta cagtggcaaa gtcttcctga
    35941 cgtaaaaaca tcattccccc cgaaagcggt cttgattctc aaggaaccac tggtaagtgc
    36001 tggcaagccc cgcttccagt gagatttcgt gataccagcc aagctgatgc aggcgcgtca
    36061 catccagcag tttgcgcggc gtaccatccg gtttgctggc atcaaaaacc acccgacctt
    36121 tgtaacccac cactttggcg atggtttgcg ccagctcgcg gatagtgcag tcaacgcccg
    36181 tgccgacgtt aatgtgcgac agcatcggtt gggtattctc cagccagact tcatgcgcca
    36241 gctccatgac atgaatgctc gccgccgcca tatcatcgac gtgcaggaat tcacgcatcg
    36301 gtgtaccgct gccccatacc accacgtccg gcgcattctg tgccgtcgcc tcgtggaagc
    36361 gacgcagcaa tgctggtatc acatgggagt tactcgggtg gaagttgtca tgcggcccgt
    36421 acaggttggt cggcatgaca gagcggtaat cgcgtccata ctggcggttg taagattcgc
    36481 acagtttgat cccggcgatt ttggcaatgg cgtaaggctc gttggtcggc tccagcgtgc
    36541 cctgcaataa ctcgctttct gccatcggct gttttgccag tttcgggtag atgcaggacg
    36601 atccgagaaa catcagtttg ttcacgtcgt tctgatgcgc ggcgtgaatg atgttgctct
    36661 caatcatcat gttctggtag atgaaatccg ccggataggt gttgttggca acaatgccgc
    36721 ccactttcgc cgccgccaga tagacctggt caatgcgttc gctggcaaag aaatcatgca
    36781 cggcgcggct gtccagcagg ttcagttcat cgcgggtacg taataccagc tccacatcac
    36841 cgcgctgttc gagctgccgc ttgatggcag aaccgaccat cccacaatgg ccagcgataa
    36901 aaatgcgttg tttacttatg cttatgactc cagcgcgatc gccacgtcgt agccgtgaga
    36961 tttcagcaga gagtgttttt tcgccgcttc gaggtcatta gccaccattt cagacaccat
    37021 ctctctgagg gtgatttccg gtttccagcc cagtttttcg tgcgctttgg tcgggtcgcc
    37081 gagcagcgtt tcaacttctg ccggacggaa gtaacgcgga tcaacggcga taatcacatc
    37141 acccggttta acgcccggcg cgtcatgccc ggtgacggaa accacaatgc ccttctcttc
    37201 aacacccgtg ccttcaaagc gcagtttgat gcccagctgt gctgccgcca tttccacaaa
    37261 ctgacgtacg gagtactgaa cgccggtcgc gataacgaaa tcttccggct gttcctgttg
    37321 cagcatcatc cactgcattt ttacgtagtc tttggcgtgg ccccagtcac gcagggaatc
    37381 catattgccg aggtacaggc acgactccag cccctgggcg atgttggcga ttgcgcgggt
    37441 gattttgcgg gtaacgaagg tttcgccgcg gcgcggggat tcatggttga agagaattcc
    37501 gttacaagcg tacatgccgt aggattcgcg gtagttaacg gtgatccagt aggcgtacag
    37561 tttggcgacc gcatacggag atcgcgggta gaacggcgtg gtttctttct gcggaatttc
    37621 ctgcaccaga ccatacagtt cagaggtgga agcctgatag aaacgagttt tcttttccag
    37681 accgaggaag cggatcgcct ccagcaggcg cagcgtaccc atcgcgtcga cgtcagcggt
    37741 atattctggt gactcaaaag agaccgcaac gtggctcatt gcgcccaggt tgtacacttc
    37801 atccggctgt acttcacgca aaatgcgcgt caggttagag gtatcactca ggtcgccata
    37861 atgcagatgg aatttcgggt tgcaggtgtg cggatcctga taaatgtgat ccacgcgctc
    37921 ggtgttgaat gacgatgcgc gacgcttaat accatgcacc tcgtaacctt tttccagcag
    37981 aaactctgcc aggtaagaac cgtcttgtcc ggttacaccg gtgatgagag cgacttttga
    38041 catgtcttat tcctctgtat ttttgaattt attcagtttc aacgcgttcg cgtatcacca
    38101 ctgcgggatt cccccggcaa accacatttg ccggaagcga tttaaaaaca ctgcttcgtg
    38161 cacccacgac ggtgccgtcg ccgattgtga cgcctggggc aacaaagaca tcggttgcca
    38221 gccagcattt ctcgccaatc acaataggcg tggcgttaat ggtgaaatgt tgacttgcat
    38281 ggtcgtggct accggtgcat aaataacttt tttgcgatat caccgaatgt gcgccaatgg
    38341 ttatttcacc gagggtatat aaattgacgt catcgccaac ccacgcgtaa tcacctaagg
    38401 ttaatttcca cggataggta atttttactg acggacgaat aactacgttt tttcctattt
    38461 ttgctccgaa taaacgtaat aaaaaagccc gccagcgata caatacttgt ggcgaccagg
    38521 caaatattgt tgcctgtact gcccaccata attgcacttt aatagcgttg ccgccccgga
    38581 accctttcgg caccgagaaa ccgcttaaat cttgcattgg ttttccttat attcagactt
    38641 tgttatataa ggctttagtc ttacctgtcg tacgttggcg taaatgccag gataattctg
    38701 cccagaagcc aggtacatgt aatatctgcc gttggacttt tttggcgtca gca
//
LOCUS       DADMGC010000020        37725 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.020, whole genome shotgun sequence.
ACCESSION   DADMGC010000020 DADMGC010000000
VERSION     DADMGC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.020"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..2005
                     /gene="speF"
                     /locus_tag="JNZ73_001059"
     CDS             <1..2005
                     /gene="speF"
                     /locus_tag="JNZ73_001059"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HAY8731427.1"
                     /translation="NEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASHYETQLRP
                     PFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLCNADVAMG
                     DLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHK
                     SNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQRAKEARPF
                     RLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCSPLLLDLN
                     ENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFY
                     PLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQS
                     YETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPYKLLLTTPGIDARNGEYEAFGV
                     PATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLLESDAPLA
                     EVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHVSMNPQEA
                     NYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGCAVLRYFSALEEGIN
                     LLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2002..3321
                     /gene="potE"
                     /locus_tag="JNZ73_001060"
     CDS             2002..3321
                     /gene="potE"
                     /locus_tag="JNZ73_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HAY8731428.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVQWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSITMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLPLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="JNZ73_001061"
     CDS             complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="JNZ73_001061"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HAY8731429.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="JNZ73_001062"
     CDS             complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="JNZ73_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HAY8731430.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5773..6537
                     /gene="ybfF"
                     /locus_tag="JNZ73_001063"
     CDS             5773..6537
                     /gene="ybfF"
                     /locus_tag="JNZ73_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY8731431.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6608..6970
                     /gene="ybfE"
                     /locus_tag="JNZ73_001064"
     CDS             6608..6970
                     /gene="ybfE"
                     /locus_tag="JNZ73_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HAY8731432.1"
                     /translation="MYYGALSIRAEAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            7110..7640
                     /gene="fldA"
                     /locus_tag="JNZ73_001065"
     CDS             7110..7640
                     /gene="fldA"
                     /locus_tag="JNZ73_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HAY8731433.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7850..7936
                     /gene="uof"
                     /locus_tag="JNZ73_001066"
     CDS             7850..7936
                     /gene="uof"
                     /locus_tag="JNZ73_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HAY8731434.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7929..8375
                     /gene="fur"
                     /locus_tag="JNZ73_001067"
     CDS             7929..8375
                     /gene="fur"
                     /locus_tag="JNZ73_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HAY8731435.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8459..8785)
                     /locus_tag="JNZ73_001068"
     CDS             complement(8459..8785)
                     /locus_tag="JNZ73_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731436.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8822..10228)
                     /gene="chiP"
                     /locus_tag="JNZ73_001069"
     CDS             complement(8822..10228)
                     /gene="chiP"
                     /locus_tag="JNZ73_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HAY8731437.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLFHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSYFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYNDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(10807..12471)
                     /gene="glnS"
                     /locus_tag="JNZ73_001070"
     CDS             complement(10807..12471)
                     /gene="glnS"
                     /locus_tag="JNZ73_001070"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAY8731438.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(12674..14620)
                     /gene="nagE"
                     /locus_tag="JNZ73_001071"
     CDS             complement(12674..14620)
                     /gene="nagE"
                     /locus_tag="JNZ73_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HAY8731439.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            14953..15753
                     /gene="nagB"
                     /locus_tag="JNZ73_001072"
     CDS             14953..15753
                     /gene="nagB"
                     /locus_tag="JNZ73_001072"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY8731440.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            15813..16961
                     /gene="nagA"
                     /locus_tag="JNZ73_001073"
     CDS             15813..16961
                     /gene="nagA"
                     /locus_tag="JNZ73_001073"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY8731441.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            16970..18190
                     /gene="nagC"
                     /locus_tag="JNZ73_001074"
                     /gene_synonym="nagR"
     CDS             16970..18190
                     /gene="nagC"
                     /locus_tag="JNZ73_001074"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HAY8731442.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALKERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18238..18980
                     /gene="nagD"
                     /locus_tag="JNZ73_001075"
                     /pseudo
     CDS             18238..18980
                     /gene="nagD"
                     /locus_tag="JNZ73_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            19377..21041
                     /gene="asnB"
                     /locus_tag="JNZ73_001076"
     CDS             19377..21041
                     /gene="asnB"
                     /locus_tag="JNZ73_001076"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HAY8731443.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21421..21497
                     /locus_tag="JNZ73_001077"
     tRNA            21421..21497
                     /locus_tag="JNZ73_001077"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21455..21457,aa:Met,seq:cat)
     gene            21506..21590
                     /locus_tag="JNZ73_001078"
     tRNA            21506..21590
                     /locus_tag="JNZ73_001078"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21540..21542,aa:Leu,seq:tag)
     gene            21614..21688
                     /locus_tag="JNZ73_001079"
     tRNA            21614..21688
                     /locus_tag="JNZ73_001079"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21646..21648,aa:Gln,seq:ttg)
     gene            21723..21797
                     /locus_tag="JNZ73_001080"
     tRNA            21723..21797
                     /locus_tag="JNZ73_001080"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21755..21757,aa:Gln,seq:ttg)
     gene            21813..21889
                     /locus_tag="JNZ73_001081"
     tRNA            21813..21889
                     /locus_tag="JNZ73_001081"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21847..21849,aa:Met,seq:cat)
     gene            21938..22012
                     /locus_tag="JNZ73_001082"
     tRNA            21938..22012
                     /locus_tag="JNZ73_001082"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21970..21972,aa:Gln,seq:ctg)
     gene            22050..22124
                     /locus_tag="JNZ73_001083"
     tRNA            22050..22124
                     /locus_tag="JNZ73_001083"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22082..22084,aa:Gln,seq:ctg)
     gene            complement(22278..23453)
                     /gene="ubiF"
                     /locus_tag="JNZ73_001084"
     CDS             complement(22278..23453)
                     /gene="ubiF"
                     /locus_tag="JNZ73_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HAY8731444.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGVGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            23599..25023
                     /gene="miaB"
                     /locus_tag="JNZ73_001085"
     CDS             23599..25023
                     /gene="miaB"
                     /locus_tag="JNZ73_001085"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAY8731445.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            25137..26216
                     /locus_tag="JNZ73_001086"
     CDS             25137..26216
                     /locus_tag="JNZ73_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAY8731446.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYLAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AREEAEQKRKAALAAERKREEQEQK"
     gene            26213..26680
                     /gene="ybeY"
                     /locus_tag="JNZ73_001087"
     CDS             26213..26680
                     /gene="ybeY"
                     /locus_tag="JNZ73_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAY8731447.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            26770..27648
                     /gene="corC"
                     /locus_tag="JNZ73_001088"
     CDS             26770..27648
                     /gene="corC"
                     /locus_tag="JNZ73_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HAY8731448.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFGMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            27673..29211
                     /gene="lnt"
                     /locus_tag="JNZ73_001089"
     CDS             27673..29211
                     /gene="lnt"
                     /locus_tag="JNZ73_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HAY8731449.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            29608..30515
                     /gene="gltI"
                     /locus_tag="JNZ73_001090"
                     /pseudo
     CDS             29608..30515
                     /gene="gltI"
                     /locus_tag="JNZ73_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
     gene            30685..31425
                     /gene="gltJ"
                     /locus_tag="JNZ73_001091"
                     /pseudo
     CDS             30685..31425
                     /gene="gltJ"
                     /locus_tag="JNZ73_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
     gene            31425..32099
                     /gene="gltK"
                     /locus_tag="JNZ73_001092"
     CDS             31425..32099
                     /gene="gltK"
                     /locus_tag="JNZ73_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HAY8731450.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            32099..32824
                     /gene="gltL"
                     /locus_tag="JNZ73_001093"
     CDS             32099..32824
                     /gene="gltL"
                     /locus_tag="JNZ73_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HAY8731451.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            32942..33877
                     /gene="rihA"
                     /locus_tag="JNZ73_001094"
     CDS             32942..33877
                     /gene="rihA"
                     /locus_tag="JNZ73_001094"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HAY8731452.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTLGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            33961..35631
                     /locus_tag="JNZ73_001095"
     CDS             33961..35631
                     /locus_tag="JNZ73_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HAY8731453.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDKQRKHTRLAADLAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(35691..37142)
                     /gene="djlC"
                     /locus_tag="JNZ73_001096"
     CDS             complement(35691..37142)
                     /gene="djlC"
                     /locus_tag="JNZ73_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HAY8731454.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(37139..>37725)
                     /locus_tag="JNZ73_001097"
     CDS             complement(37139..>37725)
                     /locus_tag="JNZ73_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY8731455.1"
                     /translation="SSSWGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWK
                     ALIAGGSERGLIYTQFVAQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWL
                     QSRVYARAHHYYHSWMHYFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFE
                     ELAAGSDRFYATLPWQPLTVQPECPVTLKDVSDL"
BASE COUNT         9200 a         9676 c         9577 g         9272 t
ORIGIN      
        1 aaatgaagaa cgtgtaccag cagaatatct gccacgtatt tctggtgtct tcgagcattg
       61 cgaatcacgg aaagagtttt atggacgtca gttagaaacc gctgctagcc attatgaaac
      121 gcaactgcgc ccgcccttct tccgcgcact ggtcgattat gtcaatcagg gcaatagcgc
      181 ttttgactgc ccgggtcatc agggaggtga gtttttccgt cgtcatccgg cagggaatca
      241 gttcgtggag tacttcggag aaacgctgtt ccgttccgac ttatgcaacg cagacgtagc
      301 aatgggcgat ctgctgattc acgaaggcgc gccatgcatt gcacagcaac atgcggcaaa
      361 agtgtttaat gccgataaaa catacttcgt tctaaatggc acttcatctt ctaacaaagt
      421 ggttttaaac gccctgctaa caccgggtga tctggtgctg tttgatcgca ataaccacaa
      481 atctaaccac cacggagcgt tgctacaggc tggtgcaaca ccggtttatc tggaaacggc
      541 acgtaacccg tatggcttta tcggtggcat tgatgcgcac tgttttgaag aaagttacct
      601 gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa gaggctcgtc ctttccgcct
      661 cgctgtgatt cagttaggca cctacgacgg tacgatttat aacgcccgcc aggtggtgga
      721 taaaattggt catctgtgtg actacattct gtttgactcc gcatgggttg gctatgagca
      781 atttatcccg atgatggctg actgttcgcc gctgttgctg gatcttaatg agaacgatcc
      841 gggtattcta gttacgcaat ccgtacataa acagcaggct ggtttttctc aaacttcaca
      901 aatccataaa aaagacagcc acatcaaagg gcaacagcgt tatgtaccgc acaaacgcat
      961 gaacaacgcc tttatgatgc acgcctccac cagcccgttc tatccgctgt ttgccgcact
     1021 ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt aatatgtgga tggattgtgt
     1081 ggtaaatggc attaatgccc gcaaactgat cctcgataac tgtcagcata ttcgtccgtt
     1141 cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat gaaacagcgc aaattgcggt
     1201 tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg cattcttttg aaggctatgc
     1261 agagaatcaa tactttgtcg atccttacaa actgttgctg acaaccccag gtattgatgc
     1321 acgtaacggc gaatatgaag cgttcggtgt acccgcgacg attcttgcta acttcctgcg
     1381 cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc atcctcttcc tgctgactcc
     1441 ggcagaagat atggccaaac ttcagcaact tgttgccctg ctggtacgct tcgaaaaact
     1501 gcttgagtcc gacgcgccat tagcagaagt gctgccttcc atctacaaac agcatgaaga
     1561 gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa atgcatgatt tgtatgcccg
     1621 ccacaacgtg aaacaactgc aaaaagagat gttccgcaaa gcgcacttcc cacacgtcag
     1681 catgaatccg caagaagcca actacgccta tttacgcggt gaagtggagc tggttcgtct
     1741 gccggatgcc gaaggtcgta tcgctgccga aggggcgctt ccttatcctc cgggtgtgct
     1801 gtgtgttgtt ccgggtgaaa tctggggttg tgctgttttg cgttacttca gcgctctgga
     1861 agaagggatc aacctgctgc caggttttgc accggagctg cagggtgtct atatcgaaga
     1921 acatgatggt cgtaagcaag tttggtgcta tgtcatcaag cctcgtgatg cgcaaagcac
     1981 cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga acaaaatggg cgtcgttcag
     2041 ttaaccatac tgacgatggt caacatgatg ggctccggta tcatcatgct gccgacaaag
     2101 cttgccgaag ttgggacaat ttcaattatc tcctggctgg tgacagccgt aggctcaatg
     2161 gcactggcat gggcattcgc aaaatgcggt atgttcagcc gtaaatcagg tggtatgggc
     2221 ggttatgccg agtatgcttt cggtaaatcc ggtaacttta tggcgaacta tacctacggc
     2281 gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg cggttggtta cggcaccgaa
     2341 ctgctcggcg cgagtttgtc gccagtgcag attggtcttg cgaccatcgg ggtgcagtgg
     2401 atttgtaccg tggctaactt tggtggtgcg cgcattaccg ggcaaatcag tagcattacc
     2461 gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta tcggctggtt ctggtttagc
     2521 ccgacgcttt acgttgattc ctggaatccg catcatgcac cgttcttcag tgcagtaggt
     2581 tcttccatca ctatgacgct gtgggctttt cttggtctgg agtctgcgtg tgcgaatact
     2641 gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg tactcggcgg tacgttaggt
     2701 gcggcggtga tttatatcgt ctccaccaac gtgattgcag ggattgtgcc taatatggag
     2761 ctggcaaatt caacggcacc atttggcctg gccttcgcac agatgttcac accagaagtg
     2821 ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg gttcgctgct tggctggcag
     2881 ttcaccattg cccaggtgtt taaatcttca tctgatgaag gctacttccc taaaattttc
     2941 tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt tgaccattgt gattatccag
     3001 agcgggttgc cgctgatgac gattagcccg tcgctaaaca gtcagttcaa cgtgctggtt
     3061 aacctggccg tggtgaccaa tatcattccg tatattctgt caatggcggc attagtcatt
     3121 attcagaagg tcgctaatgt gcccccctca aaagcgaaag ttgcaaactt tgttgctttt
     3181 gttggcgcga tgtatagctt ttatgcgctg tactcatccg gggaagaagc catgctgtac
     3241 ggttccatcg tgaccttcct tggttggaca ctgtatggtc tggtatcacc acgctttgaa
     3301 ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg gcgatcttgc gaccgccctt
     3361 tttttattaa atgtgtttac gcgtttttca gaacttcgct aacaatctca accgcttctt
     3421 tctcaatctg cttgcgatgt tcttcaccga ggaagctttc gcagtagatc ttatatgcgt
     3481 cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc agtcatcact ttcagaccgc
     3541 caatagaagc accgttgccc ggagcagcag tcaggcgcgc ggtgatcggg tcacctgcca
     3601 gggtgctggc gctcaccatt tccggagaca gcttagacag cgccgctttt tgtgcggaag
     3661 tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa gcgttttgcc agttcgttgt
     3721 agtgttcctg cgggttctta ccggtgacag cggtgatttc cgccgccagc agacacataa
     3781 tgatgccgtc cttgtcggtg gaccacggcg tgccgtcgaa acgcaggaag gaagcccctg
     3841 cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag accatcaaca aaccatttga
     3901 aacctaccgg tacttctacc agcttacggc ccaagtcatt gaccacacgg tcgatcatcg
     3961 cagaagaaac cagcgtttta ccgacagcaa catctttgcc ccactgcgga cgatgctgga
     4021 acaggtagtt gatcgccacc gccaggtagt ggttcggatt catcaaacct gccggagtga
     4081 cgataccgtg acggtcataa tccgggtcgt tagcaaacgc cagatcgaac ttatcacgca
     4141 gtgccagcag acccgccatc gcacactcgg aggagcagtc catacggatc gcaccgtctt
     4201 tatcgaggtg cataaagcgg aaggtttgat cgacctgatc gttaacgatg gtcaggttga
     4261 ggttgtaata ctcgccaatg cgcttccagt attcgatacc ggaaccgccc aacggatcaa
     4321 cgcccagcgt caggcccgct ttctgaatcg ccgccatatc aacgatatcg gccagccctt
     4381 ccacgaacgg ctgcaccaga tcctgctctt tcacatagcc ggatgccatc gcttcgtcga
     4441 gggagatacg cttcacgcct ttcaggccat cggccatcag tgcgttggcc ctgtcttcca
     4501 ccactttggt gacgttagta tcagccgggc caccatttgg cggattgtac ttgataccac
     4561 catcttccgg cgggttatgg gacggtgtaa tcacgatacc gtctgccagc gggccacctt
     4621 ttttattgtg aaccaggatg gcattggaaa cggcaggcgt cggagtgaag ccattgtttt
     4681 cctgcacaat gacatcaacg ccgttcgctg ccagcacttc cagtacggaa atgaacgcag
     4741 gctcggacag ggcgtgagta tctttaccca catagcaagg gccagtgatg ccgtttttcg
     4801 cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg cggctcgtta aagctgtggc
     4861 gcgctgcact gccacggtga ccggaagtac cgaatttcac cgcgtgctcc gcattccctg
     4921 cttctggttt cagtacataa tattgcgccg tcagttgggc gacgttaatc aaatcactct
     4981 gttgtgcagg ttggcctgca cgattgtgga ttgccattgc tttgtccttt gtctgcaacg
     5041 ttttagatag ttccgcaaac cttctcaatc aattccgccg ggaattgcat cgactgcatg
     5101 atgtgttcga tcatgctgca tttacggccg gtgttggtgt tggtgatcac ccaatacggc
     5161 gtgcctggca catgtttcgg cttggtctga ttaccatttt tcagcagcgt ttgttcatct
     5221 gccgcaaagt acacgcgtgt acgaccgtgc aacgattccg ttgcttcggc aaacgcctgg
     5281 gcgtcaagag aatatagtgt agacaacagc agcataaagc gattgaccgc tcgcttttgc
     5341 tctgcgtatt cgtccgaaag cagaagttca cgcattgcgc gaaccttgtc tttaatcgtt
     5401 ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc gaacctcttt cgtcaccgga
     5461 gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc gtaaaatgtc ggatgcgctc
     5521 tcgccgatat gcttagtgtg gctggcaata tagctgtaga gttcatcatc aacttcaatc
     5581 gttttcatct taatccagtg cagtgtctta gctgaataca agtcgccaag agtatatgaa
     5641 taaatcccgg cagcggatag cgtcaagcct ggcttgcaga aaaactcgga ataaacctta
     5701 tttgttgcag ccctgtggca caatggtcaa cataataccc taacccgaca acgcgaaaaa
     5761 aagaactttg ccatgaaatt aaatatccgc gcgcaaactg cacaaaacca gcacaataat
     5821 tctcccatcg ttcttgtcca tggtctgttt ggtagcctcg acaaccttgg cgtactggct
     5881 cgcgatctgg taaacgatca caatatcatc caggttgata tgcgtaacca cggtctttca
     5941 ccaagagagc cggtaatgaa ttacccggcg atggcgcagg atcttgttga taccctggat
     6001 gcactgcaga tcgacaaagc aacatttatc ggtcactcca tgggcggtaa agcggtaatg
     6061 gcacttactg cactggcccc cgatcgcatc gataaactgg tggcgatcga tatcgcaccg
     6121 gtcgactatc acgtacgccg tcatgatgag attttcgcag ctatcaacgc ggtcagtgaa
     6181 tcggacgcac aaactcgcca gcaagcagca gcaataatgc gccagcatct taatgaagaa
     6241 ggggtgattc agtttctgtt gaaatctttt gttgacgggg agtggcgctt taacgtaccg
     6301 gtattgtggg atcagtatcc gcatattgtg ggttgggaga aaatcccggc atgggatcac
     6361 cctgccctgt ttatccctgg cggcaattct ccgtatgtta gcgagcagta ccgtgatgat
     6421 ttactggcac aatttccaca ggcacgggcg catgtgattg caggcgcggg tcactgggtc
     6481 catgctgaaa aaccggatgc ggtattacgc gctatccgtc gctatctcaa tgattaacgc
     6541 aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg tgggcgttgg cgtgagtcat
     6601 cgcgctgatg tattatggcg cgctatccat ccgggcagag gcctggctga ttgtttcccc
     6661 cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt acgacattag atctgttcgc
     6721 gcacgagcgt cgaccgggac gaccgaaaac taatccgctt tcgcgcgatg aacagctgcg
     6781 tattaataaa cgcaaccagc taaaacgcga caaagtacgt ggccttaagc gtgtcgaact
     6841 gaagctgaac gcggaagctg tcgaggcgct gaacgagctg gcggaatcgc gtaatatgag
     6901 ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg gcggcgctgc gtagtcaggg
     6961 catcgtttaa atttccactt tcatgtagca cagtgtgcag tcctgctcgt ttgctgattc
     7021 cgcaagactg cctgttctgc tatgattgcc tttatccgtg ggcaattttc cacccccatt
     7081 tcaataagtt tcaagaggtt atttcactca tggctatcac tggcatcttt ttcggcagcg
     7141 acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa acagcttggt aaagacgttg
     7201 ccgatgtcca tgacattgca aaaagcagca aagaagatct ggaagcttac gacattctgc
     7261 tgctgggcat cccaacctgg tattacggcg aagcgcagtg tgactgggat gacttcttcc
     7321 cgactctcga agagattgat ttcaatggta aactggttgc gctgtttggt tgtggtgacc
     7381 aggaagatta cgccgaatat ttctgcgacg cattgggcac catccgcgac atcattgaac
     7441 cgcgcggtgc aaccatcgtt ggtcactggc caactgcggg ctatcatttc gaagcatcaa
     7501 aaggtctggc agatgacgac cactttgtcg gtctggctat cgacgaagac cgtcagccgg
     7561 aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat ttctgaagag ttgcatctcg
     7621 acgaaattct taatgcctga tgtgatgcgg cgtagactca tgtctacgcc gtattaatag
     7681 ataatgccaa tcaaaataat tgctacaaat ttgtaacttt tgctgttgta cctgtacaat
     7741 gtcccagtgt tcaagtggcc ttgccgttgt aaatgtaagc tgtgccacgt ttttattaac
     7801 aatatttgcc agggacttgt ggttttcatt taggcgtggc aattctataa tgatacgcat
     7861 tatctcaaga gcaaattctg tcacttcttc taatgaagtg aaccgcttag taacaggaca
     7921 gattccgcat gactgataac aataccgccc taaagaaagc tggcctgaaa gtaacgcttc
     7981 ctcgtttaaa aatcctggaa gttcttcagg agccggacaa ccatcacgtc agtgcggaag
     8041 atttatacaa acgtctgatc gatatgggtg aagaaattgg tctggctacg gtatatcgcg
     8101 tactgaacca gtttgacgac gctggtatcg tcacccgcca caattttgaa ggcggtaaat
     8161 ccgtatttga gctgacacag caacatcacc acgatcacct gatttgcctc gactgcggca
     8221 aggttatcga atttagtgat gattccatcg aagcgcgtca gcgtgaaatt gccgcaaaac
     8281 atggcattcg cctgactaac cacagtctct atctttacgg tcactgtgcc gaaggcgatt
     8341 gccgcgaaga tgagcacgcg cacgaaggca aataagccag cctgaacgag aaaagccaac
     8401 ctgcgggttg gcttttttat acaagggaaa taagaaaagc gagagttaca gctctcactt
     8461 atttgttatt actgcgtatt tcctgccaga ccttatcaca atcggctttc accgcctgat
     8521 catttccggt ttgcgttgca cgcagacact gctgataatc cagtacgcgt acactttcct
     8581 ggtcggcaaa ctgctgatgt tgtttctctt tcttcagcac gtttaacacg ctctggcagg
     8641 cttcaatttt ttctggcgaa ccttgcgcgg tgttgataca ggcgctgtat gcctctttca
     8701 gacggctgtc ttcctgtggt gcggttgatt gcgcacaagc taccagcccc gatgccatca
     8761 tggcgatgag aatcagtttt ttcattgaat gcggcaggca caaaacctgc cgcgtcgggc
     8821 atcagaagat ggtgaatggt gcgattacca taaatttcac gtcacgctca tcctggaaga
     8881 tgttaccgta accgccgccc cagcttggga tgtcggagtg gttgtcgtat tcggtgaagt
     8941 gcagtttgaa catcgtgcct ttggcgcgac cgtcctgaat ggtgtagacc gcatccaggc
     9001 tgtaggcaga ctcttcaata gtccggtttt tgtcgttgta cgcatccgga ttgctctgcc
     9061 aggtcgcagg tttagcatcc catgcgtaaa cgtaggaagc gccgatggcg aagcctggaa
     9121 gattccagtt tttcaggtca tacatcgcac cgaagaagac cgctttttcg ccgttagcgt
     9181 tgaagtagga acggttatcc caccagatat ccaggcggcc gttggaggaa gcgtaggttg
     9241 gggtcatacg ttgcaggaag tatccctgct gactgtcagc cttaacccag gtgccttcga
     9301 ggcgcaaatc cactacgtca gccgcccggt aaccaaaggt caacgcttgc agccaagcgg
     9361 tgccgtcata aagatcgttg acgctgcgat cgtcaacttt atcgcgggta ccatagaact
     9421 ggtagctggt ggttaacggg ctaccggcga tatcaaattt gtagctggct ttggcaaaat
     9481 attgatcgat atacccttcc gcctgaccaa atgccgcttc cagtacgaag ttatttttga
     9541 agtcgtattt cgcaccgatg gagtgcagat aatcaacttt ggtggtttta tcgttctgat
     9601 aaaactcatc catttccaga tgccacggcg ctttgtattc gttggtccac atgtaggaga
     9661 aactcaaggc accagcatcg ccgtaatcaa aattcgcccc ggcttccgcc ccctgataag
     9721 tacccggcat aaagctccag tgcggcgcta acagcgtttg accggttggc tgaatgtaac
     9781 ctgccctcgc ccaaaccgga ccgtatttaa atttggccgc agctttatac aggcttatac
     9841 cgcttttgtc gccggaccag tcttcgtcat aggctttatt actttttgaa aacgcgattt
     9901 cgttcgggtg ggagctgtcg ccgttttccg ccatttcaat cgccgtaaac gcagcaatat
     9961 caaggccgaa catatcagca gcataaccgg actgaaaatc gaggttggca ttccaggtgg
    10021 agtgaaaaag gttggttttg tatttgtcgc cgtcggtaac atctttacgg tcgcgttcac
    10081 gctgccagta atagataccg ccggttaagg ttgaatcgtc gatgaatcct tcggcgcgag
    10141 cctccggcat tgccataaag cccgacattg ctgtaacacc ggcaatagcc agcgccagcg
    10201 tactacgttt gccactaaac gtacgcatgg gttaatcctc tttgacgtat aaattgctgc
    10261 accaaaggtg aacagcgaaa aaaaataaaa attaaaaaag tagcgttgag aaaagtctcg
    10321 ctgactacag gaacagacta tttttcgcga cgcgaattat caatctcttt ctgtaaccag
    10381 aatgtaagaa catgagcaac cgcacatatt ttgatgattt ttgttacaag gcttaagttt
    10441 gcggttgaaa ggcttgcaga agaataaaaa cgtgaaattc cagcaagatg aaagcggtta
    10501 cattttattt aattatgttt tgtgatttta aatttatttt atggataaat tgcgaagcgg
    10561 aattaattcg cgaagaaaag cagaaaaaac gccgcagagc gcggcgtttg ggattgtttg
    10621 ctatttcttg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaagca cgcaaattca
    10681 atatattgca gagatcatgt aggcctaata agcgaagcgc atcaggcaat ttagcgtttg
    10741 aaatttattg tctatttatt gccggatgcg acgtaaatgc ctcacccggc ttggtagaac
    10801 ttaaaattac tcgcctactt tcgcccaggt atcacgcagc ccaacggtgc ggttaaatac
    10861 cggtttttcg gccgtggaat ggcggctgtc gaggcagaag taaccttcac gctcaaactg
    10921 gaatgcttta cccgctaccg catctttcag cgacggttca gcaaagccct gtttgatcac
    10981 cagcgattcc gggttaatca ccgacaggaa atcatccgca gcacctgggt taggcacgct
    11041 gaacagacga tcatacaaac ggatttcaac cggcagcgca tgtgccgcgc tcacccagtg
    11101 aataacacct ttgactttac gaccatctgc cggatctttg cttaaggtgt cggcgtcata
    11161 agtacagaag atggtggtga tattaccttc ggcatctttc tccacgcgtt cagccttaat
    11221 gacgtaagca ttacgcagac gcacttcttt acccagcacc agacgtttgt actgcttgtt
    11281 agcttcttcg cggaaatcgg cgcgatcgat ccaaatttca ccgctaaacg gcacctgacg
    11341 gctccccatt tccggtttgt tcggatggtt cggcatggtg accatttcgc cttcgccctg
    11401 atagttttcg ataaccagtt tcaccggatc gataactgcc attgcgcgcg gcgcattttc
    11461 gttgagatct tcacggatgc aggattccag cgacgccatc tcaatggtgt tgtcctgctt
    11521 ggtcacgccg atgcgtttgc agaactcacg aatagaagcc gcagtgtaac cacgacgacg
    11581 cagaccggaa atggtcggca tacgcgggtc atcccagcct tcaacgtgct tgtcggtcac
    11641 cagcaggttc agcttacgct tggacatcac agtgtattcc agattcaggc gcgagaattc
    11701 gtactggcgc ggatgaacag gaatggtgat gttatccagt acccagtcgt acagacgacg
    11761 gttgtcctgg aactcaagcg tacacagaga gtgcgtaata ccttccagcg catcgctgat
    11821 gcaatgggtg aagtcgtaca tcgggtagat gcaccacttg ttgccagtct ggtggtgttc
    11881 agcaaattta atgcggtaca gcaccggatc gcgcattacg ataaatggcg acgccatgtc
    11941 gattttcgca cgcaggcagg ctttaccttc ttcaaaacca ccggcacgca ttttttcgaa
    12001 cagcgccaag ttctcttcaa cgctgcggtc gcggtacggg ctgtttttac ccggctgcgt
    12061 cagggtaccg cggtattcgc ggatctgttc cggtgtcagt tcatcaacgt acgccaggcc
    12121 tttattgatc agttcgatcg cataggcgtg gagctgatca aaataatcgg aggagtaacg
    12181 gacgttacca gaccagtgaa aacctaacca ctctacgtcg tttttgatcg aatcgacata
    12241 ctcgatatct tcttttaccg ggttagtgtc gtcgaaacgc aggttgcact ggcctttata
    12301 gtcctgggcg atcccgaagt tcaggcagat agatttcgca tggccaatat gcagatagcc
    12361 attcggctcc ggcgggaaac gggtgtgtac tgtggtgtgc ttaccactgg ccagatcttc
    12421 atcgatgatc tgacggataa agttagtcgg gcgggcttct gcctcactca tcgtggattc
    12481 ctcaaagcgt aaacaacgta taacggcgta tgatcttata agcgggacag gctgacaact
    12541 gttagttttt taaaattgat aatctaacaa ggattatggg gatgattgat gaaaaaaaaa
    12601 gcggcagaga agatctccac cgcttgtcat tgttggatgc gacgctgccg tgtcgcatca
    12661 ggcataaagc agattacttt ttgatttcat acagcggtgt ttgacccgcc acaacatggc
    12721 cctgagcttt aatgatcaag ccactgaaat cgtcgatatt gctgcaaacc accgggctaa
    12781 tcatcgagcg ggcgttagcg ttcaggtaat ccagatccat ttccagaatc ggttgccctg
    12841 cgcttacctg cgcaccctct tccaccagac gtttaaagcc tttaccttcc agcgctacgg
    12901 tgtcgatacc catatggacg acgatctccg cgcctttttc ggtttccagg cagaacgcgt
    12961 ggttggtgtt gaagattttc acgattgtcc ctgcggctgg tgatacgacg attttatctg
    13021 tcggtttcac cgccacaccg tcacccaccg ctttgctggc gaatgcttcg tcaggaacct
    13081 gatccagtgc cacgacatca ccggtaatcg gcgataccag ctccgcgata gataccgcgt
    13141 ttggtacagc ctgcggtttt gctacaggcg cggcagttgc cggagttgct tcagctgacg
    13201 cagcggctac cggaccacgg gcaacgactt tcttcatcgc atcgccgatg gattctgctt
    13261 tcgcgccaac aatcacctga atagtctgtt tgttcagttt cactacacca gaagcaccca
    13321 gacgtttaca catcgtatcg ttgacgcggg cagagtcagc cacggtcagg cgcagacggg
    13381 taatacaggc atcaatcgct ttcaggttgt cagtaccgcc aaccgcagca atatagttgg
    13441 ttgccagttg agtcagacct tcttcagtat tgctgttggc ttcttcagta acgatctcgt
    13501 cttctttatc ttcacgaccc ggcgttttca ggttgaacat gcggataacc aaactgaaca
    13561 ccacgaagta gatagcgaag aagataacgc ccatcaccag cagcatccag acgttctggc
    13621 tggcggccgg caggttatac atcaacgcgt agtcgatagc acccgcagag aaagagaagc
    13681 cggcatggat acccagcagc gttgccacaa acaggctgat accggtcagc agtgcgtgca
    13741 ggaggtacag cagcggcgca aggaacatga acaggaattc cagcggctca gtcacaccgg
    13801 tcaggaacgc agtaacagca acagaaagca gcatcccgcc aaccatcgga cgacgctctt
    13861 tcggtgctgc gaagtacatc gccagcgccg cacccggcag accgaacatc atgatcggga
    13921 agaagccgga catgaacatc cccgcggtgc cgtcaccagc gtagaaacgg ttgatgtcgc
    13981 cgtggaaaac cgtacccgcc gcgttggtga attcaccaat ctggaaccag gcgatggtgt
    14041 tcagcacctg atgcagaccg gttgggatca gcagacggtt gatgaaacca aagataccgg
    14101 aacccagcgc gcccgcagaa acgatccact cgccgcctgc atggatagcg tgctgtaccg
    14161 gcggccagac gtaaccaaaa atggccgcca gcaccagaca gaagaagccg gtggcgatcg
    14221 gcacaaagcg tttgccgccg aagaagctca ggaagtccgg cagtttaata tcggaccaac
    14281 ggttataggc tgcgccacca accagaccgg taatgatacc cgccagtaca cccatgttaa
    14341 tttctgggtt gatggtcacc atcgctttgg ttaacacaaa gtaacctacc gcacccgcca
    14401 gcgccgccgc acctgcgctg tctttcgacc agctggatgc cacaccgatg gcgaagatta
    14461 atgcgaggtt atcaaaaatt gcaccgcccg cctgggcaat aaacgcaacg ttaagtaaat
    14521 ctggctgacc gaatcgcagc aacagcgccg ccaccggcag caccgcgata gggagctgta
    14581 acgccctacc gagtcgctgg aaaaaaccta aaatattcat cttattcccc ctacgagaac
    14641 cctatttggc tcgtttaaag ccgtattttt attttgctgc aaattttact gccgatgttc
    14701 tgtaatcaga ttgttagatc atctgctaca gagtgtgtga aaatttaatt cgtatcgcaa
    14761 attaaacgcg tgtcttttgt gagttttgtc accaaatatc gttatcatca ctccctttta
    14821 ctggctaaac cagaaaactt attttatcat tcaaaaaatc aagtcggatt gacgcctgtc
    14881 tgcgcaaatc caggttacgc ttaaagatgt ctaatccgcc aacggcttac attttactta
    14941 ttgaggtgaa taatgagact gatccccctg actaccgctg aacaggtcgg caaatgggct
    15001 gctcgccata tcgtcaatcg tatcaatgcg ttcaaaccaa ctgccgatcg tccgtttgta
    15061 ctgggcctgc cgactggcgg cacgccgatg accacctata aagcgttagt cgaaatgcat
    15121 aaagcaggcc aggtcagctt taagcacgtt gtcaccttca acatggacga atatgtcggt
    15181 ctgccgaaag agcatccgga aagctactac agctttatgc accgtaattt cttcgatcac
    15241 gttgatattc cagcagaaaa catcaacctt ctcaacggca atgccccgga tatcgatgcc
    15301 gagtgccgcc agtatgaaga aaaaatccgt tcttacggaa aaattcatct gtttatgggc
    15361 ggtgtaggta acgacggtca tattgcattt aacgaaccgg cgtcttctct ggcttctcgt
    15421 actcgtatca aaaccctgac tcatgacact cgcgtcgcga actctcgttt ctttgataac
    15481 gatgtcaatc aggtgccaaa atatgccctg actgtcggtg ttggtacact gctggatgcc
    15541 gaagaagtga tgattctggt gctgggtagc cagaaagcac tggcgctgca ggccgccgtt
    15601 gaaggttgcg tgaaccatat gtggaccatc agctgtctgc aactgcatcc gaaagcgatc
    15661 atggtgtgcg atgaaccttc caccatggag ctgaaagtta agactttaag atatttcaat
    15721 gaattagaag cagaaaatat caaaggtctg taattgttat ccctgccctg gttccttgct
    15781 cagggcaata ttttttaaaa tcgggggtca gaatgtatgc attaacccag ggccggatct
    15841 ttaccggcca cgaatttctt gatgaccacg cggttgttat cgctgatggc ctgattaaaa
    15901 gcgtctgtcc ggtagcggaa ctgccgccag agatcgaaca acgttcactg aacggggcca
    15961 ttctctcccc cggttttatc gatgtgcagt taaacggctg cggcggcgta cagtttaacg
    16021 acaccgctga agcggtcagc gtggaaacgc tggaaatcat gcagaaagcc aatgagaaat
    16081 caggctgtac taactatctg ccgacgctta tcaccaccag cgatgagctg atgaaacagg
    16141 gcgtgcgcgt tatgcgcgag tacctggcaa aacatccgaa tcaggcgtta ggtctgcatc
    16201 tggaaggtcc gtggctgaat ctggtaaaaa aaggcaccca taatccgaat tttgtgcgta
    16261 agcctgatgc cgcgctggtc gatttcctgt gtgaaaacgc cgacgtcatt accaaagtga
    16321 ccctggcacc ggaaatggtt cctgcggaag tcatcagcaa actggcaaat gccgggattg
    16381 tggtttctgc cggtcactcc aacgcgacgt tgaaagaagc aaaagccggt ttccgcgcgg
    16441 ggattacctt tgccacccat ctgtacaacg cgatgccgta tattaccggt cgtgaaccgg
    16501 gcctggcggg cgcgatcctc gacgaagctg acatttattg cggtattatc gctgatggcc
    16561 tgcatgttga ttacgccaac attcgtaacg ctaaacgcct taaaggcgac aaactgtgtc
    16621 tggttaccga cgccaccgcg ccagcaggtg ccaacattga acagttcatt tttgcgggta
    16681 aaacaatata ctaccgtaac ggactttgtg tggatgagaa cggtacgtta agcggttcat
    16741 ccttaaccat gattgaaggc gtgcgtaatc tggtcgaaca ttgcggtatc gcactggatg
    16801 aagtgctgcg tatggcgacg ctctatccgg cgcgtgcgat tgacgttgaa aaacgtctcg
    16861 gcacgctcgc cgcaggtaaa gtagccaacc tgaccgcatt cacacctgat tttaaaatca
    16921 ccaagaccat cgttaacggt aacgaggtcg taactcaata agagaaagta tgacaccagg
    16981 cggacaagct cagataggta atgttgatct cgtaaaacag cttaacagcg cggcggttta
    17041 tcgcctgatt gaccagtacg ggccaatctc gcggattcag attgccgagc aaagccagct
    17101 tgcccccgcc agcgtaacca aaattacgcg tcagcttatc gaacgcgggc tgatcaaaga
    17161 agttgatcag caggcctcca ccgggggccg ccgcgctatc tccatcgtca ccgaaacccg
    17221 caatttccac gcaatcggcg tacggcttgg tcgtcatgac gccaccatca ctctgtttga
    17281 tctcagcagc aaagtgctgg cagaagaaca ttacccgctg ccggaacgta cccagcagac
    17341 gctggaacat gccctgctga atgccattgc tcagtttatt gatagctacc agcgcaagct
    17401 gcgcgagcta atcgccattt cggtgatcct gccagggctt gttgacccgg acagcggcaa
    17461 aattcattac atgccgcata ttcaggtaga aaactggggg ctggtagaag cactgaaaga
    17521 acgttttaaa gtgacctgtt tcgttggtca cgatatccgt agtctggcgc tggcggagca
    17581 ctacttcggt gcaagtcagg attgcgaaga ctccattctg gtgcgtgtcc atcgcggaac
    17641 cggggccggg attatctcta acgggcgcat ttttattggc cgcaacggta acgtcggtga
    17701 aattggccat attcaggtcg aaccgctggg tgaacgctgc cactgcggca actttggctg
    17761 cctggaaact atcgccgcca acgctgccat tgaacaacgg gtgttgaatc tgttaaagca
    17821 gggctaccag agccgtgtgc cactggacga ctgcaccatc aaaactatct gcaaagccgc
    17881 gaacaaaggc gatagcctgg cctcggaagt gattgagtat gtcggtcgtc atctgggtaa
    17941 aaccatcgcc attgctatca acttatttaa tccgcaaaaa attgttattg ctggtgaaat
    18001 caccgaagcc gataaagtgc tgctccctgc tattgaaagc tgcattaata cccaagcgct
    18061 gaaggcgttt cgtactaatc tgccggtggt acgttctgag ctggatcacc gctcggcaat
    18121 cggcgctttt gcgctggtaa aacgcgccat gctcaacggt attttgctcc agcatttgct
    18181 ggaaaattaa tgtgctttta tagtggcgct tattgttgtc aatattctgg gtagtccatg
    18241 accattaaaa atgtaatttg cgatatcgac ggcgtgctga tgcacgataa cgtcgccgta
    18301 ccgggtgcag cggaattttt gcacgggatt atggataaag gcctgccgct ggtgttgctg
    18361 accaactatc cttcgcagac tgggcaagat ctggcgaacc gctttgccac tgccggtgtc
    18421 gatgtgccgg atagcgtgtt ttatacctct gcgatggcga ccgccgattt cctgcgtcgc
    18481 caggaaggca agaaagcgta tgtggtgggc gaaggcgcac tgattcatga actgtacaaa
    18541 gccggtttca ctattaccga tgtgaaccct gatttcgtga ttgttggcga aacacgttcc
    18601 tacaactggg acatgatgca taaagcagcc tatttcgtcg ctaacggtgc acgctttatc
    18661 gccaccaacc cggacacggt ttttatcccg cttgtggcgc gttgtgtgca gggattgaga
    18721 aaatctccgg gcgcaaaccg ttctatgttg gtaagcccag cccgtggatc atccgcgcgg
    18781 cattaaacaa aatgcaggcg cattcggaag aaacagtgat tgtcggcgat aacctgcgta
    18841 ccgatattct ggcgggcttc caggccggtc tggagacgat tctggtgctt tctggtgttt
    18901 catcgctcga cgatatcgac agtatgccgt tccgcccaag ctggatttac ccgtcagtcg
    18961 ctgaaatcga cgttatctga ataccaccca cgtctgcgga cgtggttggc tctgatgaca
    19021 aacgccaaac tgcctgatgc gctacgctta tcaggcctac gcagctcctg caatatattg
    19081 aattttcatg cttttgtagg ccgtataagg cgttcacgcc gcatccggca tgaacaaatc
    19141 gcactttatc agcaatttgg cccccgtaaa atataaccat tcaataaaac ccgctaaaaa
    19201 ttatcgattc atcaataaat acatcctcac accagaccct ttttcaccaa tcgacaatca
    19261 ccattacgtt ttttattttt gcactgccaa atagcttgcg cacagtgctg ctttgcggca
    19321 tttttttaaa caagcaaaca caacaagcaa caaataccag gttaacggag aaggttatgt
    19381 gttcaatttt tggcgtattc gatatcaaaa cagacgcagt tgagctgcgt aagaaagcac
    19441 tcgagctgtc acgcctgatg cgtcatcgtg gcccggactg gtccggtatt tatgccagcg
    19501 ataacgccat tctcgctcac gaacgtctgt caattgttga cgttaacgcg ggggcgcaac
    19561 ctctctacaa ccaacaaaaa acccacgtac tggcggtaaa cggtgaaatc tacaaccacc
    19621 aggcattgcg cgctgaatat ggcgatcgtt atcagttcca gacagggtct gactgtgaag
    19681 tgatcctcgc gctgtatcag gaaaaagggc cggaatttct cgacgacttg cagggcatgt
    19741 ttgcctttgc cctgtacgac agcgaaaaag atgcctacct gattggtcgc gaccatctgg
    19801 ggatcatccc actgtatatg gggtatgacg aacacggtca gctgtatgtg gcctcagaaa
    19861 tgaaatcgct ggtgccagtt tgccgcacga ttaaagagtt cccggcgggg agctatttgt
    19921 ggagtcagga cggcgaaatc cgttcttatt atcatcgcga ctggttcgac tttgatgcag
    19981 tgaaagataa cgtgaccgac aaaaacgagc tgcgtcaggc actggaagat tccgttaaaa
    20041 gccatctgat gtctgatgtg ccttacggtg tgctactttc tggtggtctg gattcctcaa
    20101 ttatttccgc tatcaccaag aaatacgcag cccgtcgcgt ggaagatcag gaacgctctg
    20161 aagcctggtg gccgcagtta cactcctttg ctgtaggtct gccgggttca ccggatctta
    20221 aagcagccca ggaagtggca aaccatctgg gcacggtgca tcacgaaatt cacttcactg
    20281 tacaggaagg tctggatgcc atccgcgacg tgatttacca catcgaaact tatgatgtga
    20341 ccactattcg cgcttcaaca ccgatgtatt taatgtcgcg taagatcaag gcgatgggca
    20401 ttaaaatggt gctgtccggt gaaggttctg atgaagtgtt tggcggttat ctttacttcc
    20461 ataaagcgcc caacgctaaa gaactgcatg aagagacggt gcgtaaactg ctggccctgc
    20521 atatgtatga ctgcgcgcgc gccaacaaag cgatgtcagc ctggggcgtg gaagcacgcg
    20581 ttccgttcct cgacaaaaaa ttccttgatg tggcgatgcg cattaacccg caggataaaa
    20641 tgtgcggtaa cggcaaaatg gaaaaacaca tcctgcgtga atgttttgag tcatacctgc
    20701 ccgcaagcgt ggcctggcgg cagaaagagc agttctccga tggcgtcggt tacagttgga
    20761 tcgacaccct gaaagaagtg gcggcgcagc aggtttctga tcagcaactg gaaactgccc
    20821 gcttccgctt cccgtacaac acgccgacct caaaagaagc gtatctgtac cgcgagatct
    20881 ttgaagaact gttcccactt ccgagtgccg ctgagtgcgt gccgggcggt ccttccgtcg
    20941 cgtgttcttc agctaaagcg atcgaatggg atgaagcgtt caaaaaaatg gacgatccgt
    21001 ctggtcgtgc ggttggcgtt caccagtcgg cgtataagta agaaatgcgg tactgccccg
    21061 gagaaatccg gggctgtttt agttattgcg ctgcttgttg attctgatat gcagtaagga
    21121 aatgttccgg gtttgccaca tcactgtttg acaactcatc actgtaggcc agtatttcat
    21181 agattaattt cccaccacta ttgttgaatt ccgaatttca tcctgtacga caaaagaatg
    21241 gctgtagggt ggggaaaaaa cacaaagtaa acaataattg acgaatatag cgccacgctg
    21301 ttcgcaacct aaccaaacag tcactttcga gcaattttcc ttgaaaaaga ggttgacgct
    21361 gcaaggctct atacgcataa tgcgccccgc aacgcagata aggtatcgcg aaaaaaagat
    21421 ggctacgtag ctcagttggt tagagcacat cactcataat gatggggtca caggttcgaa
    21481 tcccgtcgta gccaccatct tttttgcggg agtggcgaaa ttggtagacg caccagattt
    21541 aggttctggc gccgcaaggt gtgcgagttc aagtctcgcc tcccgcacca ttcaccagaa
    21601 agcgttgtac ggatggggta tcgccaagcg gtaaggcacc ggtttttgat accggcattc
    21661 cctggttcga atccaggtac cccagccatc ttcttcgagt aagcggttca ccgcccggtt
    21721 attggggtat cgccaagcgg taaggcaccg gtttttgata ccggcattcc ctggttcgaa
    21781 tccaggtacc ccagccatcg aagaaataat ctggctacgt agctcagttg gttagagcac
    21841 atcactcata atgatggggt cacaggttcg aatcccgtcg tagccaccaa attctgaatg
    21901 tgtcgaatac gttcggcaaa ttcaaaaacc aatttgttgg ggtatcgcca agcggtaagg
    21961 caccggattc tgattccggc attccgaggt tcgaatcctc gtaccccagc caatttattc
    22021 aagacgctta catcgtaagt gcacccagtt ggggtatcgc caagcggtaa ggcaccggat
    22081 tctgattccg gcattccgag gttcgaatcc tcgtacccca gccacattaa aaaagctcgc
    22141 ttcggcgagc tttttgcttt tctgcgttca ttcaatgtcg aatgcgatgt tgacacgtct
    22201 tatccttcaa tgtcggatgc gacgctgccg cgtcttatcc gacctacggt tggcacgcat
    22261 ccggcaatgt tgtaaggcta caaccctaac gcatatttca gcgcctgacg tttcaacacg
    22321 ccagcacgct ctgccgccat taacccgaga ttacgcacaa aacgcagcgg cggcagatta
    22381 ttactgaatc cggcataaaa cagatccata ccactttgca taatgaagtt atccgccatg
    22441 cgccgcatct ggtaacgctt gaggacagga taactggccc acgcttcacc gtagctgcgg
    22501 gcattcacca gaacatcaat cagggcatcg acatcacgat aaccaagatt caccccctgc
    22561 cccgccagcg gatggatggt atgcgcggca tcgcccacca gcgcaagccc cggctgcaca
    22621 tactgcaacg catgtcggcg cgtcagcgga aacgcaccag cggcaagcgg tgtaacgtaa
    22681 cccagacgcg acgggaaatg cttcgcgatt tccgcctgga gttgcgccat attcatattc
    22741 tgcaactggc gaatacgcgc cggagagtca taccacacca gcgatgccca gttatcaaac
    22801 aacggcagaa acgcacgcgg tccgtccggg gtaaattgct gccaggtgct gtcgccggga
    22861 tcgttctcac actgtacgct aatcaacatg cacgactgcg catactgcca ggcatgaacg
    22921 ccaattcccg ccatctgccg cacctgagaa tttgcgccgt cggcaccaat caccagtttc
    22981 gcgcggatcg tttcaccgcc cttcagttcc agctcctgaa gatcattatc tcgatgtaat
    23041 gcaatcagcg aggttggcac acgtaacgtt actttcggat gcgcttccag cgcctgccac
    23101 aacgcctgtt gcaggactgt gttttccacc atgtagccaa gcaacggcaa ctttaattcg
    23161 gctgcgtcaa ataccacatg tgccgtttcc cactcccagg tttccagtct gcggtaagga
    23221 tggcaacgca tagcctgtac tgcatcccag acccctaccc ctttaagcaa tgataccgaa
    23281 gccgcgctga tcgccgagat ccgcacgtcc ggttggctgt cagcgacaaa cggtgcaggt
    23341 tctgcgtgct cgatcaccgt taccgaaaat ccgtgctgtg ccagccccag cgccagtgcg
    23401 ccgccgacca ttcctccgcc gacaatggca atttccgttg gttgatttgt catggtcaat
    23461 catcctgtta gcaatatgct ttaagtttac aggattttta cccccaaggg ctgataacgc
    23521 tcatactggt cacaacggca gcaaagcatt acactatgcg ccctgcattc ctggctacta
    23581 tttcgcaaga gcaagtcgat gaccaaaaaa ctccatatta aaacctgggg ctgtcagatg
    23641 aacgagtacg attcatcgaa gatggccgat ctgctggatg ccacccacgg ctatcaactg
    23701 accgacgtgg cggaagaagc ggatgtgcta ctgctgaaca cctgctcaat ccgcgagaag
    23761 gctcaggaaa aagtcttcca tcagttgggt cgctggaaac tgttaaaaga gaagaatcca
    23821 gacctgatta tcggcgtcgg tggctgcgtg gcatcgcaag aaggcgagca cattcgccag
    23881 cgcgcccact atgtcgatat tatttttggg ccgcaaacgc tgcaccgtct gccggagatg
    23941 atcaactccg tgcgcggcga ccgcagcccg gttgtagata tcagcttccc ggaaatcgag
    24001 aagtttgacc gtctgccgga accgcgcgcc gaagggccga ccgcgtttgt ctccatcatg
    24061 gaaggctgca ataaatattg cacctactgc gtggtgcctt acacccgtgg tgaagaggta
    24121 agccgtccgt ctgacgatat tctgtttgag attgcccagc ttgcggctca gggcgtgcgt
    24181 gaagtcaacc tgctcggtca gaatgtgaat gcctggcgtg gtgagaacta cgacggcacc
    24241 accggatcgt ttgccgatct gttgcgtctg gttgctgcga tcgacgggat cgatcgtatt
    24301 cgctttacca ccagccatcc gatcgaattc accgacgata tcatcgaagt gtaccgcgac
    24361 acgcctgagc tggtgagctt cctgcatctg ccggtacaga gcggttccga tcgcattctg
    24421 aacctgatgg gacgtaccca tacggcgctg gagtacaaag cgatcatccg taaactgcgt
    24481 gcggcgcgtc cggatattca gatcagttct gacttcattg ttggcttccc tggcgaaacc
    24541 accgaagact tcgagaaaac gatgaagctg attgccgacg tcaatttcga catgagctac
    24601 agctttatct tctctgcacg tccaggaacg cctgccgccg atatggttga tgatgttccg
    24661 gaagaagaga agaagcagcg tctgtatatt ctgcaagaac gtatcaatca gcaagcgatg
    24721 gcatggagcc gtcggatgct cggcaccacc cagcgtattc tggtagaagg gacttcgcgt
    24781 aagagcatca tggagctttc cgggcgtacc gaaaataacc gcgtggtcaa cttcgagggc
    24841 acaccggata tgatcggtaa attcgtcgat gtagaaatta ccgacgtcta cccgaactct
    24901 ctgcgcggta aagtggtgcg aactgaagat gaaatgggtc tgcgcatggc agaaacaccg
    24961 gagtcggtga ttgcccgcac ccgcaaagaa aacgaccttg gcgtgggtta ttatcagccg
    25021 taattctcag gcctgccgtt ccggtgggcc ttgttttttc tctctttatc cccatcttta
    25081 tcgcaatgct tgcgcctttt tcctgtggcg tgaataattt ccttataggc cggtgaatgt
    25141 tcagcttccg gcggcataca gataagagga acggtttgaa catagacact cgcgaaatca
    25201 ccctggagcc agcagacaat gcgcgtctgt tgagcctgtg cggcccgttt gatgacaaca
    25261 tcaagcagct cgaacgccgt ctcggcatcg agatcaatcg ccgcgataac cactttaaac
    25321 tgaccggccg tccgatttgc gtcaccgctg cggcagacat tctgcgtagc ctgtatgtcg
    25381 atactgcccc gatgcgcggt cagattcagg atatcgaacc ggaacagatc caccttgcga
    25441 ttaaagaagc gcgggtactg gagcaaagcg cggagagcgt gccggagtac ggcaaagcgg
    25501 tcaatatcaa aactaaacgc ggcgtaatta agccgcgcac gccaaaccag gcgcagtaca
    25561 tcgccaatat tctcgaccat gacattacct tcggcgttgg cccggcgggt acgggtaaaa
    25621 cctacctggc agtggctgcg gcagttgatg ccctggagcg tcaggaaatt cgccgtattc
    25681 tgctgactcg tccggcggtc gaagccggtg agaaactggg cttcctgcct ggcgatttaa
    25741 gccagaaagt agacccgtat ctgcgcccac tgtacgacgc gctgtttgaa atgctgggct
    25801 ttgagaaagt cgagaaactg attgagcgca acgttattga agtcgcgccg ctggcctata
    25861 tgcgtggtcg tacgctgaac gacgcgttta tcattctcga tgagagccag aacactacca
    25921 tcgaacagat gaagatgttc ctgacccgta tcggttttaa ctcaaaagcg gttatcaccg
    25981 gcgacgtcac acagatcgac ctgccgcgta atactaaatc aggcttacgt catgccattg
    26041 aagtgctggc cgatgtcgaa gagatcagct ttaacttctt ccacagcgaa gacgtggttc
    26101 gtcacccggt ggtggcgcgt atcgttaacg cttatgaagc cagggaagaa gcagaacaaa
    26161 aacgtaaagc ggcgctggca gcagaacgca agcgcgaaga acaggaacaa aaatgagtca
    26221 ggtgatcctc gatttacaac tggcatgtga agataattcc gggttaccgg aagagagcca
    26281 gtttcaaaca tggctgaatg cggtgatccc gcagtttcag gaagagtcgg aagtgactat
    26341 tcgcgtggtc gataccgccg aaagccacag tctgaatctg acctaccgcg gtaaggataa
    26401 gccgaccaac gtgctctcct tcccgtttga agtgccgcct ggcatggaga tgtcgctact
    26461 gggcgatctg gttatctgcc gtcaggtggt tgagaaggaa gctcaggagc aaggcaaacc
    26521 actggaggcg cactgggcgc atatggtggt gcacggcagt ctgcatttgt taggttacga
    26581 tcacattgaa gatgacgaag cagaagaaat ggaagccctc gaaacagaga ttatgcttgc
    26641 tctgggctat gaggatccgt acattgccga gaaagaataa tttgccaggc atttgactgg
    26701 cagacgtccc cgccgttggt gattaacgac ggcgttttga ttaactaaca tgagaaccca
    26761 tacgacgcca tgagcgacga caattcacac agtagtgaca cgataagcaa caagaaggga
    26821 tttttctccc tgttactcag ccaacttttc cacggtgaac cgaaaaaccg tgacgaactg
    26881 ctggcgctga tccgtgattc cgggcagaac gaccttatcg acgaagatac gcgcgatatg
    26941 ctcgaagggg tgatggacat cgcagaccaa cgcgttcgcg acatcatgat cccccgctcc
    27001 cagatgatta ccctgaaacg caaccagacg ctggacgaat gccttgatgt catcatcgag
    27061 tccgcccact cacgtttccc ggtgattagc gaagacaaag atcacattga agggattctg
    27121 atggcgaaag acttgctgcc gtttatgcgc agcgatgctg aagccttcgg catggacaaa
    27181 gtgttacgtc aggcggttgt cgttcctgaa agtaagcgcg tagaccggat gctgaaagag
    27241 tttcgctctc agcgttacca catggcgatc gttattgacg aattcggtgg ggtttccggt
    27301 ctggtgacca ttgaagacat cctggaactg attgttggtg agattgaaga cgagtatgac
    27361 gaagaagatg atatcgactt ccgtcagctg agtcgtcata cctggaccgt gcgcgcactg
    27421 gcttccattg aagacttcaa cgaagcgttc ggcacccact ttagcgatga agaagtcgac
    27481 actatcggtg gtctggtgat gcaggcgttt gggcatcttc cggcgcgtgg cgaaactatc
    27541 gacatcgacg gttaccagtt caaagtggcg atggccgata gtcggcgtat tattcaggtt
    27601 catgtcaaaa tcccggatga ctcaccccag ccaaagctgg atgaataaaa ccgaaactgg
    27661 atagataact acatggcttt tgcctcatta attgaacgcc agcgcattcg cctgctgctg
    27721 gcgttattat tcggtgcctg cggaacgctg gccttctctc cttacgacgt ctggcctgcg
    27781 gcgattattt cgctgatggg gcttcaggcg ttgaccttta accgtcgccc actccagtct
    27841 gccgctattg gcttttgctg gggatttggc ctctttggca gcggtattaa ctgggtctat
    27901 gtcagcatcg cgacctttgg cgggatgcct ggcccggtta acatcttcct agtggtgctg
    27961 ctggcggcgt atttgtcgct gtataccgga ctgtttgctg gcgtgctgtc gcgtctgtgg
    28021 ccgaaaacca cctggctgcg cgtggcgatt gccgcccctg ctctctggca agtgaccgag
    28081 tttctgcgcg gttgggtgct gactggcttc ccgtggttac agttcggcta tagccagatt
    28141 gatggcccgt taaaagggct ggcaccgcta atgggcgtgg aagccattaa cttcctgctg
    28201 atgatggtta gtggcctgct ggcactggcg ttggtcaaac gcaactggcg tccgctggtg
    28261 gtggccgtcg tgctgtttgc cctacccttc ccgctgcgtt acatccaatg gtttaccccg
    28321 caaccggaga aaaccattca ggtttcgatg gttcagggcg atattccgca atcgctgaaa
    28381 tgggacgaag gccagcttct taatacgcta aagatttact acaacgcaac ggcaccgctg
    28441 atgggcaaat catcgttgat tatctggccg gagtcggcga taaccgatct ggaaattaat
    28501 cagcaaccgt tcctcaaagc actggacggt gagttgcgtg ataaaggtag ctcgctggta
    28561 accgggattg tcgacgcgcg tctcaataag cagaaccgct acgataccta caacaccatc
    28621 atcacgctgg gtaaaggtgc accgtacagc tacgaatcag ccgatcgcta taacaaaaac
    28681 catctggtgc cgtttggcga gtttgtcccg ctggagtcga ttctgcgtcc gttagcaccg
    28741 ttctttgatc tgccgatgtc gtcgttcagc cgtgggccat atatccagcc gccgctgtcg
    28801 gcaaatggta ttgagcttac tgcggctatt tgctacgaga tcattctcgg cgagcaagtg
    28861 cgcgataact tccgcccgga taccgactac ctgctgacta tctccaacga tgcgtggttt
    28921 ggtaaatcta ttggtccatg gcaacacttc cagatggcgc gaatgcgtgc gctggagctg
    28981 gcgcgcccac tgttgcgcag caccaacaac ggcattacgg cggtgattgg cccgcagggt
    29041 gagattcagg cgatgatccc gcagttcacc cgcgaggtgt taaccactaa cgtgacgccg
    29101 accaccggac tcacaccata cgcacgtacc ggcaactggc cgctgtgggt gctgacggca
    29161 ctgtttggtt ttgctgctgt gttgatgagt ctgcgtcagc gacgtaaata atcccttctt
    29221 atcgtgccgg aatacatccc atatttcggc acgctcaccg gatgcggcat cagtgcatta
    29281 tccggataac aatattctcc tatccaactt tggcacatct attgctttgt tatacaaggc
    29341 aaagcttgaa ccagcatcag tacaacccgg ttgcaccaca gcaatccacc ggtagcacca
    29401 aaagagtgca ttttaagaat tttgcttcag aatggtgcgg tgcacctcgc aaaaacaaac
    29461 aataaaacat caatatcttt acatttagcc aaactaaatg ttacctaaca ggcacaacac
    29521 tgcacaataa agttgcagac gataacaaca caaacactca caacgggtat ccatgcgttc
    29581 ttaacgcaga agataaagga gttggatatg caattacgta aacctgccac agcaatcctc
    29641 gccctggcgc tttccgcagg actggcacag gcagatgacg ccgccccggc agcaggcagt
    29701 acgctggaca aaatcgccaa aaacggtgtg attgtcgtcg gtcaccgtga atcttcagta
    29761 cctttctctt attacgacaa tcagcaaaaa gtggtgggtt actcgcagga ttactccaac
    29821 gccattgttg aagcagtgaa aaagaaactc aacaaaccgg acttgcaggt aaaactgatt
    29881 ccgattacct cacaaaccgt attccactgc tgcaaaacgg cactttcgat tttgaatgtg
    29941 gttctaccac caacaacgtc gaacgccaaa aataggcggc tttctctgac actattttcg
    30001 tggtcggtac gcgcctgttg accaaaaagg gcggcgatat caaagatttt gccgacctga
    30061 aaggcaaagc cgtagtcgtc acttccggca ctacctctga agttttgctc aacaaactga
    30121 atgaagagca aaaaatgaat atgcgcatca tcagcgccaa agatcacggt gactctttcc
    30181 gcaccctgga aagcggtcgt gccgttgcct ttatgatgga tgacgctctg ctggccggtg
    30241 aacgtgcgaa agcgaagaaa ccagacaact gggatatcgt cggcaagccg cagtctcagg
    30301 aagcctacgg ttgcatgttg cgtaaagacg atccgcagtt caaaaagctg atggatgaca
    30361 ctatcgctca ggtgcagacc tccggtgaag cggaaaaatg gtttgataaa tggttcaaaa
    30421 atccaattcc gccgaaaaac ctgaacatga atttcgaact gtcagacgaa atgaaagcac
    30481 tgttcaaaga accgaatgac aaggcactga actaattaca agaaccagag gcggaaattc
    30541 cagccctctc gattgttacg tagcacggac agactatacg cctgatggtc gttccccatc
    30601 gggcctgaaa accgcaatac gctgggtaac aatcttcgag ggtagcagtt aacgctgcta
    30661 ccctcttttt tctggagtag atttatgtct atagactgga actggggtat ttttttacaa
    30721 caagccccgt tcggcaatac cacctatctt ggttgggtct ggagtggttt tcaggtgacg
    30781 atcgctttgt cgatctgcgc ctgaattatc gctttcctcg tcggttcatt tttcggcatt
    30841 ttacgtaccg ttccaaaccg ttttctctcc ggtctgggta cgttgtatgt cgaactgttc
    30901 cgcaacgtgc cgttgattgt gcaattcttt acctggtatc tggtgatccc ggagctgctg
    30961 ccggagaaaa tcggcatgtg gtttaaggcc gagctggatc ccaatattca gtttttcctt
    31021 tcatccatgc tctgcctggg gctgtttacc gcctcccgtg tttgcgaaca ggtacgcgcc
    31081 gctattcagt cgctaccgcg tgggcagaag aatgccgcgc tggcgatggg cctgacgcta
    31141 ccgcagactt atcgatacgt actgctgcct aatgcttatc gcgttatcgt cccgccgatg
    31201 acttccgaga tgatgaacct ggtgaaaaac tccgccatcg cctcgaccat cggtctggtg
    31261 gatatggcgg cgcaggcggg taaattgctc gactactcgg ctcacgcctg ggagtcgttt
    31321 accgccatca cgcttacgta tgtgttgatt aacgctttta tcatgctggt gatgacgctg
    31381 gttgaacgta aagttcgcct gcccggcaac atggggggca aataatgtac gagtttgact
    31441 ggagttccat tgttccttcc ctgccatatc tgctcgacgg gctggtgatc accctgaaaa
    31501 tcaccgtcac ggcagtcgtg attggtattt tgtggggtac catgctggcg gtgatgcgct
    31561 tgtccagctt cgcgcctgtt tcgtggtttg ccaaagccta cgttaacgta ttccgctcga
    31621 ttcctttagt catggttttg ctgtggtttt acttgattgt gccgggattt ctgcaaaatg
    31681 tgctgggatt atcgccaaaa aatgatattc gtttaatttc ggcgatggtg gcgttttcga
    31741 tgtttgaagc ggcctactat tcagagatta tccgtgccgg tattcaaagt atttcccgtg
    31801 gtcagtcgag cgccgcgctg gcgttgggga tgactcactg gcagtcgatg aaactgatta
    31861 ttctgccgca ggctttccgc gcgatggtgc cgctgctgct cactcagggc atcgtactgt
    31921 tccaggatac ctcgctggtg tatgtgttat ctctggccga tttcttccgt accgcctcaa
    31981 ccattggtga gcgtgatggt acgcaggtcg agatgatcct gtttgccgga tttgtttatt
    32041 tcgttattag ccttagcgcg tcgttgttgg tcagctactt gaaaagaagg acagcataat
    32101 gattaccctg aaaaatgttt caaaatggta tggtcacttt caggtgctga ccgactgctc
    32161 aaccgaagtg aaaaaaggcg aagtggtggt ggtttgcggc ccgtctggtt ccggcaaatc
    32221 aacgctgatt aaaaccgtca acggcctcga accagtgcag caaggtgaaa tcaccgtcga
    32281 tggtatcgtg gttaacgaca agaaaaccga tctggcaaag ctgcgctccc gcgtcgggat
    32341 ggtattccag catttcgagc tgttccctca tctgtcgatt atcgaaaacc tgaccctggc
    32401 gcaggtgaaa gtgcttaaac gcgataaagc gcccgctcgt gaaaaagcat tgaaattgct
    32461 ggagcgcgtg ggactttccg cacatgccaa taaatttccg gcgcagcttt ccggcggtca
    32521 gcagcagcgt gtggcaatcg ctcgcgcatt gtgtatggct cctattgcga tgctgttcga
    32581 cgaaccgaca tcggcgctgg atccggagat gatcaacgaa gtgctggacg tgatggtgga
    32641 actggcgaac gaaggaatga ccatgatggt ggtgacccac gaaatgggct ttgcccgcaa
    32701 agtggcgaat cgggtgatct ttatggacga gggtaaaatt gtcgaagact cgccgaaaga
    32761 cgctttcttc gatgatccga aatcggaccg cgcaaaagac ttcctcgcga aaatcctgca
    32821 ttaatcactc tggcgcgcat acccaattgc gcgccatctc acgctttatc gtttcgcccc
    32881 tcgtatctgt tacatcctgg cgttaccctt gtcgcaaaga agcacacaac aaggagcaac
    32941 aatggcactg ccaattctgt tagattgcga cccaggtcat gatgacgcta tcgcaatagt
    33001 tctcgccctc gcctcaccag agcttgatgt caaagcaatt acgtcttccg ccggaaacca
    33061 gacaccagaa aaaaccttac gcaatgttct gcgtatgctg accttgctta atcgcaccga
    33121 tattccggtg gcaggcggcg cggtaaaacc gttaatgcgt gagttgatta tcgcggacaa
    33181 tgtgcacggc gaaagcggtc tcgacggccc ggcattaccg gaaccgacgt tcgcaccgca
    33241 aaactgtacg gcggtggagc tgatggcgaa aacgctgcgc gaaagtgcgg aacctgtcac
    33301 gattgtttct accggaccgc aaactaacgt tgccttgctg ctcaatagcc atccggaact
    33361 gcatagcaaa attgcccgta tcgtgattat gggcggcgca atggggcttg gtaactggac
    33421 gcctgcggct gaatttaata tttacgttga cccggaagcg gcagaaattg tcttccagtc
    33481 agggatcccg gtggtgatgg ccggtctgga tgttactcat aaagcgcaaa tccacgttga
    33541 agacaccgag cgtttccgcg cgattggcaa ccctgtttca accattgttg ccgaactgct
    33601 ggatttcttc ctcgaatatc ataaagacga aaaatggggc tttgtcggcg caccgctgca
    33661 tgacccatgc accatcgcct ggctgttgaa acccgagttg tttaccactg ttgagcgctg
    33721 ggttggcgtg gaaacacagg ggaaatatac cctgggtatg acggttgttg attattatta
    33781 tctgactggc aataaaccga atgccactgt aattgtcgat gttgatcgtc agggctttgt
    33841 tgatttactg gctgatcgtc tgaaatttta cgcttaacac tcgtcattga ttaagatcac
    33901 cacgcgttac ctgggtggtc ttaatctatt tatctctttt tctttcaaga tataaatgca
    33961 atggataatg cagaactcgc cataggtatc gatctcggca ctaccaatag tttaattgcc
    34021 gtctggaaag acggtgccgc gcaattaatt ccaaataagt tcggtgaata tttaacacca
    34081 tccataatta gcatggatga aaataatcat attttagtcg gaaaaccggc tgtatcacgg
    34141 cgtacttcgc atccggataa aacggcagcg ttatttaaac gtgcaatggg cagtaatacc
    34201 aactggcggt taggcagcga cacatttaac gcgccagaac tgtcctcttt ggtattacgc
    34261 tcattaaaag aagatgccga agaatttctg caacgtccga ttaaagatgt ggtgatctcc
    34321 gttccggctt atttcagcga taaacaacgc aagcataccc gtttagcagc ggacttagcc
    34381 gggttaaatg cggtacgctt aattaatgaa cccacagcag ctgcgatggc gtatggcctg
    34441 catacccaac aaaatacccg ttcgctggtg tttgatctcg gtggcggcac gtttgacgtt
    34501 acggtgcttg agtacgccac gccggtgatt gaagttcacg cctccgctgg cgacaacttc
    34561 ctcggtggcg aagattttac ccatatgttg gtcgatgacg ttttaaagcg cgcggatgtc
    34621 acaaggacca cgcttaacga gagtgaactg gcagccttgt acgcctgtgt ggaagcggca
    34681 aaatgtagca atcaatcgcc aatgcacatt cgctggcagt accaggatga aacgcgggaa
    34741 tgcgaatttt acgagaacga actggaagat ttgtggttgc cgctgctcaa tcgcttgcga
    34801 gtgccgattg aacaggcgtt gcgcgatgcg cgtctgaagc cgagtcaaat cgacagtctg
    34861 gtgctggttg gcggcgcgtc acaaatgccg ctggtgcagc gaatcgccgt gcgtctgttt
    34921 ggcaaattac cgtatcaaag ttacgatccg agcaccattg tcgcgctggg cgcagcaatc
    34981 caggccgcct gccgcttacg cagtgaagat attgaagaag tgatcctcac tgatatttgc
    35041 ccttactcgt tgggcgttga ggttaaccgc cagggcgttt ccggcatttt ctcgccgatt
    35101 attgaacgaa acaccactgt gcccgtgtcg cgtgtagaaa cttattcaac catgcacccg
    35161 gaacaggatt caattacggt taacgtctat cagggagaaa accacaaagt taaaaacaac
    35221 attctggtgg aatccttcga tgtgccgttg aagaaaaccg gggcttatca gtcgattgat
    35281 attcgcttta gttatgatat caacgggttg cttgaagttg acgtgcttct ggaagacggc
    35341 agcgttaagt ccagagtgat taaccacagc ccggtaacat tgagcgcgca gcagattgaa
    35401 gagagtcgga cgcggttatc cgcattgaaa atttatccgc gcgatatgct catcaatcgc
    35461 acctttaaag ccaaactgga agagttgtgg gcgcgggcgc tgggtgacga gcgagaagag
    35521 attggccggg tgatcaccga ttttgatgcg gcgctgcagt caaacgatat ggcccgcgtc
    35581 gatgaggtca ggcggcgggc gagcgtttat ttagccattg agacctcata atcacggctg
    35641 ccggatgcgg cgtaaatgct gcatccggca tcggcgcaaa aaacacatcg tcaactaaac
    35701 aacatcgcga ttttgccgag agcataacct ataaccagca ccgttaaact gattttgata
    35761 atgctggtaa tgctctgtgg tttttcaccc tcatcatttt tttcttcatg gttttcaggt
    35821 gttggctcat cgacatcttc atcctccggc tcgagtttta ctttcgtcaa cagcatccgg
    35881 gtgataatgg cctctaatgg atgagcatct ccgccttcca gccttgtatt aagacgggcc
    35941 agcatctctt gtttcggaaa cgcttcaagt actacctgtt ccgctacccg atacaaatcc
    36001 agtaacggtg aatcttcttc tgtctcgcta ccgactctcg atttccacca cgcccatgct
    36061 ttgcgtttga agaataacga ctcaataaac gattgaccta acggcgataa ttttccgcta
    36121 tgtaaaattt ccgcccataa cagccgcgtc cgggcgctct caccaggaac atatttcagc
    36181 ggttgacctt gcgcatctac catgctccgc gcttcgataa ccatcactaa taacgcgaaa
    36241 taatccgggc aatgacgctt gctccactgc aacaacaaat tatcagcttg tgtagatggg
    36301 taactttccc agtacgcgcc gagatcagca agcaggcttt ccccctcgcc gcagtacaaa
    36361 cgttgtgcac actggtaata ccgggcatct tcgctttctg gttcttccgc ttgccactgt
    36421 ttggctacag gaatgagttc caccatacct gtttgcaccg agctgtacca gcgcaacagt
    36481 ttgcgatgta actttgcatc atcaggaatg aaccacggtc cttccatcgc caaaaacgca
    36541 gtgacatagt caggatggta acggcagcaa cgttccagcg cgaagtaata ttcaacagtc
    36601 tggttctgta acgcgacggg atattccaga aggctaagga aatcgaaaca atcaccagcc
    36661 tgaatggcct caagaaaagc ctccctttct tcctcatcca ttccttcgct gtcattaaac
    36721 gaatgccagt tcaaacgctc tgccaacaaa gaagcacaat ttaatgacag atatcgcgct
    36781 tctatagcga ttgcacacag cggccagcgt aattgatcga catcctccat gttccaggta
    36841 tttaattgct gaataaattt ctgccaggcg gagggctgaa aacgatcact ttctgaatcc
    36901 agtaatgtcc tgaatgcacg tagtatttca tgttcagcgg tggcatcttt ttcttcatca
    36961 tctgcttcct cgacaggatt taccgccagt cgcagcgcct cttcataggc ctggcgtaat
    37021 gctttaaacc cctgcggatc ggtttcggga tgacacaacg gtaagcgagc aagataagcc
    37081 tggcggataa tgtctatttg cgtcgtgctt tcaatttcga ggatttgcca acatgttttc
    37141 ataggtcgct cacatcctta agcgttaccg ggcattcagg ctggacagtt aatggctgcc
    37201 agggaagcgt ggcatagaaa cggtcgcttc ccgctgccag ctcttcgaac atccgactcc
    37261 cggcgctatc gtatttgtac agggttagcg cctcgcgtag cgaagcgttg tcttcgcact
    37321 gagaagattg ccagtagaga cgccctagtg aataggcaga aaaatagtgc atccagctgt
    37381 gatagtaatg gtgagctcgt gcatagacgc gcgattgcag ccataaactc tcttcttccg
    37441 tcagccagtt gttgagcaca ccggcccgtg acagaaaccc cattcgaaca taatcccagg
    37501 ctttgattcc cccttctcca cagcacatag cggtttgggc aacgaattgg gtataaatca
    37561 accctctctc cgagccacca gcaattaatg ctttccactc ttcaggggaa gagcgttgcc
    37621 atgtcagata taagccagct aaatcattgg catgaccatc atcagtcatc tgataaatag
    37681 tctgaattaa atcatcacga ttatttattc cccaggatga tgaga
//
LOCUS       DADMGC010000021        36918 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.021, whole genome shotgun sequence.
ACCESSION   DADMGC010000021 DADMGC010000000
VERSION     DADMGC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.021"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..174)
                     /locus_tag="JNZ73_001098"
     CDS             complement(<1..174)
                     /locus_tag="JNZ73_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HAY8731456.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(258..1142)
                     /gene="yhdJ"
                     /locus_tag="JNZ73_001099"
     CDS             complement(258..1142)
                     /gene="yhdJ"
                     /locus_tag="JNZ73_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY8731457.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     YYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1228..1524)
                     /gene="fis"
                     /locus_tag="JNZ73_001100"
     CDS             complement(1228..1524)
                     /gene="fis"
                     /locus_tag="JNZ73_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HAY8731458.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1550..2515)
                     /gene="dusB"
                     /locus_tag="JNZ73_001101"
     CDS             complement(1550..2515)
                     /gene="dusB"
                     /locus_tag="JNZ73_001101"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAY8731459.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2844..3725)
                     /gene="prmA"
                     /locus_tag="JNZ73_001102"
     CDS             complement(2844..3725)
                     /gene="prmA"
                     /locus_tag="JNZ73_001102"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAY8731460.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKTEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3737..5188)
                     /gene="panF"
                     /locus_tag="JNZ73_001103"
     CDS             complement(3737..5188)
                     /gene="panF"
                     /locus_tag="JNZ73_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HAY8731461.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5178..5420)
                     /locus_tag="JNZ73_001104"
     CDS             complement(5178..5420)
                     /locus_tag="JNZ73_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HAY8731462.1"
                     /translation="MDTSFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5529..6878)
                     /gene="accC"
                     /locus_tag="JNZ73_001105"
     CDS             complement(5529..6878)
                     /gene="accC"
                     /locus_tag="JNZ73_001105"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAY8731463.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVQSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHIEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6889..7359)
                     /gene="accB"
                     /locus_tag="JNZ73_001106"
                     /gene_synonym="fabE"
     CDS             complement(6889..7359)
                     /gene="accB"
                     /locus_tag="JNZ73_001106"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAY8731464.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8337..9311)
                     /locus_tag="JNZ73_001107"
     CDS             complement(8337..9311)
                     /locus_tag="JNZ73_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY8731465.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPSTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9463..11403
                     /gene="csrD"
                     /locus_tag="JNZ73_001108"
     CDS             9463..11403
                     /gene="csrD"
                     /locus_tag="JNZ73_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HAY8731466.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11708..12751
                     /locus_tag="JNZ73_001109"
     CDS             11708..12751
                     /locus_tag="JNZ73_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAY8731467.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12817..13920
                     /gene="mreC"
                     /locus_tag="JNZ73_001110"
     CDS             12817..13920
                     /gene="mreC"
                     /locus_tag="JNZ73_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAY8731468.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13920..14408
                     /gene="mreD"
                     /locus_tag="JNZ73_001111"
     CDS             13920..14408
                     /gene="mreD"
                     /locus_tag="JNZ73_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAY8731469.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14417..15010
                     /gene="yhdE"
                     /locus_tag="JNZ73_001112"
     CDS             14417..15010
                     /gene="yhdE"
                     /locus_tag="JNZ73_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HAY8731470.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15000..16469
                     /gene="rng"
                     /locus_tag="JNZ73_001113"
     CDS             15000..16469
                     /gene="rng"
                     /locus_tag="JNZ73_001113"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HAY8731471.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16537..20337
                     /locus_tag="JNZ73_001114"
     CDS             16537..20337
                     /locus_tag="JNZ73_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HAY8731472.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QSAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVDGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKAR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20493..21938
                     /gene="tldD"
                     /locus_tag="JNZ73_001115"
     CDS             20493..21938
                     /gene="tldD"
                     /locus_tag="JNZ73_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HAY8731473.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFSYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22066..22995)
                     /gene="aaeR"
                     /locus_tag="JNZ73_001116"
     CDS             complement(22066..22995)
                     /gene="aaeR"
                     /locus_tag="JNZ73_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HAY8731474.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23178..23381
                     /locus_tag="JNZ73_001117"
     CDS             23178..23381
                     /locus_tag="JNZ73_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HAY8731475.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23389..24321
                     /gene="aaeA"
                     /locus_tag="JNZ73_001118"
     CDS             23389..24321
                     /gene="aaeA"
                     /locus_tag="JNZ73_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HAY8731476.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24327..26294
                     /gene="aaeB"
                     /locus_tag="JNZ73_001119"
     CDS             24327..26294
                     /gene="aaeB"
                     /locus_tag="JNZ73_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HAY8731477.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26386..26658
                     /locus_tag="JNZ73_001120"
     CDS             26386..26658
                     /locus_tag="JNZ73_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731478.1"
                     /translation="MNIYAFDFNEIESQEDFYRDFSQTLGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26714..26977)
                     /gene="yhcN"
                     /locus_tag="JNZ73_001121"
     CDS             complement(26714..26977)
                     /gene="yhcN"
                     /locus_tag="JNZ73_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HAY8731479.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27342..27812)
                     /gene="argR"
                     /locus_tag="JNZ73_001122"
     CDS             complement(27342..27812)
                     /gene="argR"
                     /locus_tag="JNZ73_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HAY8731480.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28247..29185
                     /gene="mdh"
                     /locus_tag="JNZ73_001123"
     CDS             28247..29185
                     /gene="mdh"
                     /locus_tag="JNZ73_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAY8731481.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(29247..30314)
                     /gene="degS"
                     /locus_tag="JNZ73_001124"
     CDS             complement(29247..30314)
                     /gene="degS"
                     /locus_tag="JNZ73_001124"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HAY8731482.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30404..31771)
                     /gene="degQ"
                     /locus_tag="JNZ73_001125"
     CDS             complement(30404..31771)
                     /gene="degQ"
                     /locus_tag="JNZ73_001125"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HAY8731483.1"
                     /translation="MKKQTQLLSALALSAGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDEKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPSSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTNTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31925..32323)
                     /locus_tag="JNZ73_001126"
     CDS             complement(31925..32323)
                     /locus_tag="JNZ73_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HAY8731484.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            32517..33644
                     /locus_tag="JNZ73_001127"
     CDS             32517..33644
                     /locus_tag="JNZ73_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HAY8731485.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33863..34291
                     /gene="rplM"
                     /locus_tag="JNZ73_001128"
     CDS             33863..34291
                     /gene="rplM"
                     /locus_tag="JNZ73_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAY8731486.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34307..34699
                     /gene="rpsI"
                     /locus_tag="JNZ73_001129"
     CDS             34307..34699
                     /gene="rpsI"
                     /locus_tag="JNZ73_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAY8731487.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35172..35507
                     /locus_tag="JNZ73_001130"
                     /pseudo
     CDS             35172..35507
                     /locus_tag="JNZ73_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein B"
     gene            complement(35550..36917)
                     /gene="dcuC"
                     /locus_tag="JNZ73_001131"
     CDS             complement(35550..36917)
                     /gene="dcuC"
                     /locus_tag="JNZ73_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY8731488.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         8956 a         9226 c         9702 g         9034 t
ORIGIN      
        1 tctgtcctga aaattcagtt cgtcacagtt aaccagtttt agtggattaa ctgagttcat
       61 attacggcat ttgtctgttt ctgttgtcag cagttcaatc cactgcatca gtaaagtatc
      121 aaacagaaaa acgaagacag cgaaaacgac gagccaccaa tacttgcgaa tcattacctg
      181 agcctgtgca tgtttaacca agtctggcag gaaatataca cgaaatgaat aatctgaaaa
      241 ccaggcttac acttccttta ctttgcaatg agatcggggt caacttcgct taaccggctt
      301 cgttttgaca ggttgccggt ctttcttttt ttcactttcg ccagttcttc cgcggagtaa
      361 tgcgacgcga catccagccg tcgaagcccc attttgatgt actcgctgtt gatctcaata
      421 ccaatgaatt ttcgtccgct ggcgatggct acggcaccgg tagtaaagct accggcaaac
      481 gggtcgagaa cgatatcgcc tgggttggaa gaggcgagaa taatgcgttt cagtaaggct
      541 ttcggttttt gcgtcgggtg gttctcatat tcatccatca aataacgcac gcgtggaaaa
      601 tcccagacgt tacccggtac tttttgatga ttgtatggct gtggaggatt tttgcgataa
      661 tagatcaacg cgcgctgcga tccggttttg gcttctacca gaatagcatc accgttgaat
      721 gtgtagttct ttgcgtcttt caccatcatc aggatgggtt cgtacatgga gccgtagtgt
      781 tttttcgcct gtactccaga actgtcatat gaccagacga tgcgactttt gatggtaaaa
      841 agcttgcggc actggagatc gataaagggc atgttttccg tactgttcat aatgtacatg
      901 ctgccctgct ttttcagaac gcggtggcac tctgcaatca cttcaaacag ccagtcgata
      961 aacagatctt ctttccaggc ttcgatcaaa ccatcaaaat ttttaccgat gttatacggt
     1021 gggtcggcaa agagcagatc gacgctttca gtaggtagct ttttaagttc ggcaagggca
     1081 tcaccgtgaa taatggtctt agcttcatta ccaaaccggg tcggttcaca tcctgttctc
     1141 atggtcactc cctttgtgac acctataaaa aaggcgcttc cctatgccga gtagcgcctt
     1201 tttaatcaag catttagcta acctgaatta gttcatgccg tattttttca attttttacg
     1261 cagcgtacca cggttgatgc ccatcatcag cgcagcacgg gtctggttac cacgggtgta
     1321 ttgcatcacc atgtccaaca ggggctgttc tacttcagcc agtaccagct catagaggtc
     1381 attcacatcc tgaccattca gttgagcaaa atagttcttc agtgcctgtt taaccgagtc
     1441 acgcaggggt ttttgggtta cctgatcctg agagttaacg gtagaaacgg tcagtacgtc
     1501 agaatttacg cgttgttcga acatagttct gtcagctctt tatttctgtt tacgcaaaat
     1561 tttcgaagta tgcctccaac gcctccagct gttcgctggc atcctcaatg gcgttgaatg
     1621 tgcgccgaaa ctggtcattt ggagcgtgtt cctggagata ccaggaaacg tgtttacgtg
     1681 caattcggta cccttttgcc ggaccataaa agtcatgcag ttcccgaacg tgcgcgcaaa
     1741 gcaagcgctt aacctctgcc aaaggcagcg ggggcagcaa ctccccagtg tccagataat
     1801 gctggatttc ccgaaagatc cagggtcttc cctgagctgc gcggcctatc atcagggcat
     1861 ccgcccctgt atagtcgagc acagctctgg ctttaagcgg gtcagtaatg tcgccattcg
     1921 cgataaccgg aatggaaact ttctgcttaa ctgcccgaat actgtcgtac tcagcttctc
     1981 cattgaacaa acaggcgcgt gtacggccat gaatggtcag agcctgaatg ccacagtctt
     2041 cagccagttg ggcaatctct tcgcagttac ggtgttccgg tgcccagccg gtgcgaatct
     2101 tcagggtaac aggaacgtcc actgcattga cgacctcggt aaggatcgat ttaacgacat
     2161 ccgggtactg caagagggct gaacctgcga gcttgcgatt cactttttta gccgggcaac
     2221 ccatattgat atcaataatc tgggcaccgc tttccacgtt aatacgtgct gcatctgcca
     2281 tttctttcgg agcgctacca gcaatttgca cggtgcgaat accgggttca tcaatgtgca
     2341 ccatccgtaa acgagatttg tcgctttccc aaacctgtgg gttagaagac atcatctcgg
     2401 atactgtcaa tccggctccc atctcgtagc acaacgtccg aaaaggtctg tctgtaatgc
     2461 cagccatggg cgctgcgatc aggcgatttc tgagctgata ttgtccgatg cgcatgagtt
     2521 aagaaatgac catactgtga ctgcaaggcg gcgtatatta cgcatttttt gcacgagatg
     2581 aaaggccaaa ctttgaccaa tcctctgaga tggatcaaag aattgcattt aaaatgagcg
     2641 tggtgcgata attactcata aaaatcatca tattagaaaa tagtgactaa aatttacact
     2701 caaagaaatt tgagtaagtt ctcaattttt ctttatgaat gaaaattttg gcacgcaaat
     2761 ttgcgtaaat aatcggcaaa tttacgtgcc ttttgtgagc ttgctcgcac ttcgccccgc
     2821 gtcaccctac ggcgatgcga aggttaattc ttacgaccgg taatacggca ccactcttct
     2881 ttttccacga ccgggtccag tgcgaagcta tcggcataag cttcacaaac gctctctgcc
     2941 tggcttgcca gaataccgga aaggcccagc aaaccgcctg aaaccggcag gacgctgatt
     3001 aacggtgcca gttcacgtaa tgggcctgca aggatgttag cgaccaccac gtcggctttc
     3061 atttcttctg gctgatcttt cggtaagtag agttccagac ggtcagaaac gccattacgt
     3121 tcggcgttat cgcggctggc ctgaatcgcc tgcggatcga tatcaatacc aatggctttt
     3181 gctgcaccca gtttcagcgc cgcgatcgcc agaatgccgg aaccacagcc aaagtcgatg
     3241 actgttttac cggttaaatc gaggctgtcg agccattgca ggcacagaga ggtggttgga
     3301 tgggtacccg taccaaacgc cagccctgga tctaacatca cgttgacggc gttttcgtcc
     3361 ggcacatcac gccagctagg gcagatccac agtcgttcac caaagcgcat cgggtggaaa
     3421 ttatccatcc attcgcgctc ccagtcttta tcttctagtt gttcggtttt atgcgcgaag
     3481 cctgcgccga gcagcggatg gttttccaga atcgccacca cgtcgttcat atcggtttca
     3541 gcgtcgaaca gaccaatcac atcggtgtcg ccccacaggc gcgtttcgcc cggcagcggc
     3601 tcaaatactg gcgtatcgtg ggtatcctga aaagtgatag aaacggcacc cgcttccatc
     3661 agcgcatcgc taagatcttc cgcgttcgcg ccggtggtgt tcagtttcag ttggatccaa
     3721 ggcatggcaa aactctttat ttatcagtag tcaaaacggt agcttgcggg acggatgtac
     3781 cgaaacggtt tccgaccagg aaagccagca aacttagtag taacgagggc acgatagggt
     3841 ggaagcccag gtactgaata ttcagcgtcg cgagtacggc atacagcacg ccgccaacga
     3901 tcatcgcact tagcgcgcct ttggcgttgg cgcgttccca gtaaagaccc agcaccagcg
     3961 gccacaggaa aacggcttcc agcccaccga aggccagcaa attcagccag atgatcattt
     4021 ctggcggctt ccaggcggca agcagcagca acgcgccgag aactaacgta attaccgccg
     4081 acatccgctt cagacgcgtc tcgttttgca tttgatccgg acggatattc agatagagat
     4141 ctttaatgat cgtagcggaa ctttgcagca gttgggcgtt aattgtcgac atgatcgcag
     4201 ccataggtgc agccagaaag atcccggcag caaaaggtgg cagcactttt accattaacg
     4261 tcgggatcac cagatccggt acggtgagat cggggatcac tgcacgacct aacgctccgg
     4321 ctaggtgcat accgaacatc aggattgcga ctacaatcgt accgatgatg atcccccggt
     4381 gtactgcttt gctgtcttta taagagatac agcgcaccgc agtatgcggc aggccaatca
     4441 cgccaaagca caccagtacc cagaacgacg tcataaaggc aggcgacaga atatcgtcag
     4501 cgccttgtgg cgtaaccagt tgcggatcga tggtttgcaa ggtctgtact gcgttgctta
     4561 agccgccagc ggcatgtacc acgccaataa gcagcacaac ggtgccaatc agcatcacaa
     4621 gcccttgcat ggtgtcgttc agcacgctgg cgcgaaagcc accaaaggcg gtatataacg
     4681 caatgctgat accaaaaatc agcagcccgg tttcataagg aatacccgcc gcggtttcca
     4741 gcaggcgcgc accgccgata aactgcacgg tcatcgcacc aacgaacgca accagcaaac
     4801 tcaaactcgc cagccacacc agaagacgac tctggtagcg ggcaaacagc atatcgttca
     4861 gcgtcactgc attgtagcgg cgcgcaagaa tcgcaaactt cttgccgaga ataccgagtg
     4921 aaagccagac agcaggaagc tgaatcatcg ccagcaatac ccagcccagc ccgtatttat
     4981 aagcagctcc tggcccgccg ataaacgaac tggcactgat ataggtcgcg gtgagcgtca
     5041 tcgccagcac aataccgccc atagagcggc tgccgaggaa atactcatta aggaaggtgc
     5101 cggtgctccg tttacgcatc gcataaaccg agataccgaa caccaccacc agataggcga
     5161 ccagcggtag aattacttca agctgcatcg tcatcctcca gtgggatatc gcgatagata
     5221 aatttcacca tcgcccagca cagtccaata aacagtagcg gcgtcaggat gcaggccatc
     5281 tcaaaccagc gcggaaagcc ggtaaaaccg ggggcaacgc caggtaagta agcatctact
     5341 aaccaaactg ccagatacaa aagggtcagc cccagcgccc agcgcgcctc tttatgggcc
     5401 tgaacaaaac tagtgtccat tttttgtccc tgatgggtga agaaagcgag gattgtacct
     5461 tatgggggtt gtcgatcccc agtaataaaa aaggccggaa aatccggcct tttgacgctt
     5521 ttgcagtctt atttttcctg aagaccgagt tttttctcca gatagtggat gttagtgcca
     5581 ccatgctgga agttctcgtc attcatgatg cggatctgca gatcaacgtt ggttttgata
     5641 ccgtcgatga tcagctcctg cagcgcattc ttcatgcggg caatcgccac gtcacggttt
     5701 tcaccgtagc aaatcagctt accgatcatt gagtcatagt acggcggtac ggtgtagccc
     5761 gcgtagatat gagactccca acgtacgcca aaaccgccag gtgcgtggaa acgggtgatt
     5821 ttgcctgggc ttggcaggaa ggtgttcgga tcttcggcgt tgatacgaca ttccaccgca
     5881 tggccgcgaa cgtgaacttc ttcttgcttg atcgacagcg gttgaccggc agcgatacgc
     5941 agctgttctt tgatcaggtc aacgccggtg atcatttctg taaccgggtg ttctacctga
     6001 atacgggtgt tcatttcgat gaaatagaac tcgccgtttt cgaacaggaa ctcgaaagta
     6061 cctgcaccgc gatagccgat atcaacacac gctttagcgc aacgttcgcc gatgtagcga
     6121 cgcagttccg gggtaatgcc cggtgctggt gcttcttcga ccactttctg gtggcggcgc
     6181 tgcatggagc agtcacgttc cgccagatag atagcgttgc cctgaccgtc agccagtacc
     6241 tgaatctcga tgtggcgagg attctccagg tatttctcca tgtaaaccat atcgttgctg
     6301 aaagcagctt tcgcttccgc acgggtcatg gagatggatt gtgccagttc agcgtcgccg
     6361 cgcactacgc gcataccgcg accgccgccg ccgccggagg ctttgataat caccggataa
     6421 ccaatgcgtt tagcaatggc gcggttttta tccatatcgt cgcccagcgg gccgtcagaa
     6481 ccaggtacgc aaggaacgcc cgcttttttc atcgccgcga ttgcggatac tttgtcgccc
     6541 atcaggcgaa tggtttctgc tttcgggccg atgaagataa agccggagcg ttcaacctgc
     6601 tcggcaaagt tggcgttctc ggagaggaag ccgtaacccg gatggattgc tactgcgccg
     6661 gtgatttcag cggcgctgat gattgccggg atgttcagat aactttgtac tgacggagca
     6721 gggccaatac agaccgtttc atctgccagt aatacgtgtt ttagatcgcg atccgcgctg
     6781 gagtgcacag cgacagtctt gatgcccagt tctttacagg cacgaagaat acgcaatgca
     6841 atctcgccgc ggttggcaat aacaatttta tccagcatgt tcgcctcgtt actcgatgac
     6901 gaccagcggc tcgtcaaatt ctaccggttg tccactttcg accagaattg ctttcacggt
     6961 accggatttg tccgcttcga tctggttcat cattttcatg gcttcaacga tgcacagggt
     7021 atcgcccacg ttgactttct gacccacttc gatgaacgct tttgcgtccg ggcttggggt
     7081 gcggtagaaa gtaccaacca tcggggaacg tacgatgtga ccactgattt ccgctgctgc
     7141 tggcgcttcc atggaaggaa cggtcgccgg agcggctgcg ttagattgag ctggctgctg
     7201 catcattggt gcagcgtaag cttgttgcat cacagggaaa cttgcggcag gagctgcacg
     7261 gctaatgcgt actgactctt cgccttcaga aatttccagt tcggagatgc ctgattcttc
     7321 aaccagctcg atcagttttt taatcttacg aatatccatg agtgggttcc gtactctttg
     7381 tttagtgtga ttgtgacagg cgtttcaccg ccgtctgtaa agcgtatgcc ccgacagctg
     7441 tatgcatagc gataaattcc agcaggccgg gacgccggtc tattttgccc cagagtgtca
     7501 atgttagact tgacggacat tgtgcagcga tctgctgcta tcctccggca aaaaacaaaa
     7561 tataccttcg ttgcgccaat gtcacctttt ccgcagcgca aaaactgcgt cagggaggac
     7621 gaggtcgcac attataacga tttcgtagca attggcagct aaatactggt cttatcaggg
     7681 aagataatca acagctaaca tgtaaataac cttcaacacc gtgtaatttg caacaagccg
     7741 cacaattcac gaaattagcg ccatcatcga cggaacttct tataacgtaa ggtgaaaagc
     7801 tgaaaagcca gccctgcgaa gatgaggggc tgcaatgaga taatcttcac agaccacaaa
     7861 taatgtaggg atgccaggat cgcgacaaga tagacgacat tacgcagcgg ttgctgggga
     7921 ttacccaatt ttcactgcat cgtctggata gatgtgaatg ctaaagcacg caaaatcaac
     7981 caactgacaa ttctcagcat taaataggtt cgagtggtta atttgtagtc aaataaaatc
     8041 agattattca ccccatggta ccagaaatca gtaacaggtt aagcgtagtg tcgcccaggc
     8101 aaagcaccat aaacctaaca ggcgacggag gcgtatcaat aatggctgtt tagaggagta
     8161 tcaatgcgca tcaatttgtg gtcagtcgaa atcatgactt tccagtttgg atgatgttgt
     8221 acaacttcct gaccaaggtg ttcgacgaaa gctgtccggc taaccgcttt tcccgttaac
     8281 cgattgaata atgatgtcaa aatgagttcc ctctcttatt attccttgct aaatggttag
     8341 ttaaccttca ccagcgtgcg accttggatc tggttattaa tgatggcctc ggcaaagttc
     8401 ggtgcctctg acagagatat ctcttttgcc gcctgggtat agaatgattc cggtaaatcg
     8461 gcgaccagtc gctgccaggc ttgtgcgcgg cgttctggtg gcgtcattac tgaatccacc
     8521 ccttgcaaac ggacattacg cagaataaat ggcatgaccg tgcttggcag agtaaaacca
     8581 cccgccagac cacaggccgc cacgcagccg ccgtaattca tttgcgccag cacttttgcc
     8641 aggactttgt cgccaacagt gtcaattgcc ccagcccaga cttgtttttc cagaggacgg
     8701 gattcggcaa actcatcacg agggagaata cggctggcac ctaaactttt cagatattca
     8761 tgggtacttt cgcgaccgga aacggcaacg acctgataac ccaacttatg cagcagcgcc
     8821 acggcggtac tgccgacgcc accactggca cccgtcacga caatctcccc gtcctgcggg
     8881 cgaacaccgg catcttccag cgccatcaca cacagcatgg cggtaaaacc ggcagtaccg
     8941 ataatcattg ctttacgcgc gtccagccct tgcggcatgg caaccagcca gtcacctttc
     9001 actcgcgcct gctccgccag cccaccccag tggttttcac caacgcccca gccagtgagt
     9061 aacacctcct gaccggcatg aaaacgcgga tcttcgctgg tgcgtacagt tccggcaaaa
     9121 tcgatcccag gaatcatcgg aaaattacgg atgatttttc ccttaccggt aatcgccagc
     9181 gcatctttat agttcaggct cgaccagtga acatcgaccg tgacatcgcc ctccggcagg
     9241 cgactttcgt ccagagtctg tactgatgcg agagttttgc cgtcctgctg ttctaaaagt
     9301 aacgcctgca taagtggtcc tcatgtgcat gatggattgg aaaataattt ctgaagacta
     9361 tactcgctaa tggaaaagca aagccgatga agcgcaataa attgcgagat aaatctgatt
     9421 tgctagtatg cccgcttcct cactatcgga gttaacacaa ggatgagatt aacgacgaaa
     9481 ttttcggcct ttgttacgct gctcaccggg ttaacaattt ttgtgacttt gctgggctgt
     9541 tcgctaagtt tctacaacgc cattcagtat aagtttagtc atcgcgttca ggcggtggcg
     9601 acggcgattg atacccacct tgtgtcgaat gacttcagcg cattaaggcc acaaattacc
     9661 gaattaatgg tgtcagcaga tatcgttcgt gtagacctgc tgcatggtga taaacaggtt
     9721 tataccctgg ccagaaatgg tagttatcgt ccggttggca ccagcgatct gtttcgtgaa
     9781 ctgagcgttc cgttgataaa gcatccgggg atgtcgttgc gtctggttta tcaggatccg
     9841 atgggcaact atttccattc gttgatgacc accgcgccgc tcacgggggc gattggcttt
     9901 atcattctta tgctctttct ggcggtacgc tggttacaac ggcaacttgc cgggcaagaa
     9961 ttgctggaaa cccgggctac tcgtatctta aacggtgagc gtggctctaa tgtgttggga
    10021 accatctatg aatggccgcc cagaaccagc agtgcgctgg atacgctgct tcgtgaaatt
    10081 cagaacgcac gcgaacaaca cagccgtctt gatacgctga tccgctctta tgccgcacag
    10141 gacgtgaaaa ccggcctcaa taaccgactc tttttcgata atcagttagc aacgttactg
    10201 gaagatcagg agaaagtagg tacccacggg atcgtgatga tgattcgtct gccggatttc
    10261 aatatgttga gcgatacctg ggggcacagc caggttgaag aacagttctt cactctgacg
    10321 aatctgctgt cgacatttat gatgcgctac cctggcgcac tgctggcgcg ttaccaccgc
    10381 agtgattttg ctgcgctgtt accgcaccgg acgttaaaag aggcagagag catagccagt
    10441 cagttaatca aagccgtcga taccttgccg aacaataaaa tgctcgatcg cgacgatatg
    10501 atccacattg gtatctgtgc ctggcgtagt ggtcaggata ccgagcaggt aatggaacat
    10561 gcagagtctg ccacgcgtaa tgcgggattg cagggtggca atagctgggc tatttatgat
    10621 gactcgttgc ctgaaaaagg acgcggtaat gttcgctggc gtacgcttat cgagcaaatg
    10681 ctcagtcgcg gcggcccgcg cctttatcaa aaaccggcgg ttactcgcga aggtcaggtt
    10741 catcatcgcg aactcatgtg ccgcatcttc gatggtaatg aagaggttag ctcggcggag
    10801 tatatgccga tggtcttgca gtttggctta tcggaagagt atgaccgtct gcaaatcagc
    10861 cgtcttattc cactattgcg ttactggcca gaggaaaatc tggcgattca ggttaccgtt
    10921 gagtcgctga ttcgtccgcg ttttcagcgt tggctgcgcg atacgttaat gcaatgtgaa
    10981 aaatcacaac gaaaacgcat aattattgaa cttgcagagg ccgatgtagg tcaacatatc
    11041 agtcgtttac aacctgttat tcgtttagtg aatgctttag gggtacgggt agccgtcaac
    11101 caggctggtt tgacgctggt aagcaccagt tggatcaaag aacttaatgt tgagttactc
    11161 aagctccatc cggggctggt caggaacatt gagaagcgaa cggagaacca gctgctggtt
    11221 caaagcctgg tggaagcctg ctccgggacc agcacccacg tttacgccac cggcgtgcgt
    11281 tcgcgaagcg agtggcagac cctgattcag cgcggtgtta caggcgggca aggggatttt
    11341 ttcgcgtcct cacagccact tgatactaac gtgaaaaaat attcacaaag atactcggtt
    11401 taacctgccg tttaatccgt tttcacgtag aataacgcgc gctgcgtctc atgggagtgt
    11461 gcttgtctgc tcgccagatt gttgcagcac atatgcagat gaatgacctt acgcggttgc
    11521 aaacaggcga ggaatgctgc tgatgcatta agcctttctg gactcaggca gagatttgta
    11581 acaaaggaaa cgaactgcac taattttcac cgtagcagat gatttttgcg ccttgtcgct
    11641 gctgcgtgtg gttggtaaag taagcggatt ttcttttccg ccccagcttt caggattatc
    11701 ccttagtatg ttgaaaaaat ttcgtggcat gttttccaat gacttgtcca ttgacctggg
    11761 tactgcgaat accctcattt atgtaaaagg acaaggcatc gtattgaatg agccttccgt
    11821 ggtggccatt cgtcaggatc gtgccggttc accgaaaagc gtagctgcag taggtcatga
    11881 cgcgaagcag atgctgggcc gtacgccggg caatattgct gccattcgcc caatgaaaga
    11941 cggcgttatc gccgacttct tcgtgactga aaaaatgctc cagcacttca tcaaacaagt
    12001 gcacagcaac agctttatgc gtccaagccc gcgcgttctg gtttgtgtac cggttggcgc
    12061 gacccaggtt gaacgccgcg caattcgtga atccgcgcag ggcgctggtg cccgtgaagt
    12121 cttcctgatt gaagaaccga tggctgccgc aattggtgct ggcctgccgg tttctgaagc
    12181 gaccggttct atggtggttg atatcggtgg tggtaccact gaagttgctg ttatctcctt
    12241 gaacggcgtg gtttactcct cttccgtgcg cattggtggt gatcgtttcg acgaagctat
    12301 catcaactat gtgcgtcgta attacggttc tctgatcggt gaagccaccg cagaacgtat
    12361 caagcacgaa atcggttcgg cttacccggg cgatgaagtc cgtgaaatcg aagttcgtgg
    12421 ccgtaacctg gcagaaggtg ttccacgcgg ttttaccctg aactccaacg aaatcctcga
    12481 agcactgcag gaaccgctga ccggtattgt gagcgcggta atggttgcac tggaacagtg
    12541 cccgccggaa ctggcttccg acatctccga gcgcggcatg gtgctcaccg gtggtggcgc
    12601 actgctgcgt aaccttgacc gtttgttaat ggaagaaacc ggcattccag tcgttgttgc
    12661 tgaagacccg ctgacctgtg tggcgcgcgg tggcggcaaa gcgctggaaa tgatcgacat
    12721 gcacggcggc gacctgttca gcgaagagta atcggatgca ggcaggggaa gcgtctgttt
    12781 accctgcctg gtctgatacg agaatacgca taacttatga agccaatttt tagccgtggc
    12841 ccgtcgctac agattcgcct tattctggcg gtgctggtgg cgctcggcat tattattgcc
    12901 gacagccgcc tggggacgtt cagtcaaatc cgtacttata tggataccgc cgtcagtcct
    12961 ttctactttg tttccaatgc tcctcgtgaa ttgctggatg gcatatcgca gacgctggcc
    13021 tcgcgtgacc aactagaact tgaaaaccgg gcgttacgtc aggaactgtt gctgaaaaac
    13081 agtgaactgc tgatgcttgg acaatacaaa caggagaacg cgcgtctgcg cgagctgctg
    13141 ggttccccgc tgcgtcagga tgagcagaaa atggtgactc aggttatctc cacggttaac
    13201 gatccttata gcgatcaagt tgttatcgat aaaggtagcg ttaatggcgt ttatgaaggc
    13261 cagccggtca tcagcgacaa aggtgttgtt ggtcaggtgg tggccgtcgc taaactgacc
    13321 agtcgcgtgc tgctgatttg tgatgcgacc cacgcgctgc caatccaggt gctgcgcaac
    13381 gatatccgcg taattgcagc cggtaacggt tgtacggatg atttgcagct tgagcatctg
    13441 ccagcgaata cggatattcg tgttggtgat gtgctggtga cctccggtct gggcggtcgt
    13501 ttcccggaag gctatccggt cgcggttgtc tcttccgtaa aactcgatac ccagcgcgct
    13561 tatactgtga tccaggcgcg tccgactgca ggactgcaac gtttgcgtta tctgctgctg
    13621 ctgtggggcg cggatcgtaa cggcgctaac ccgatgacgc cggaagaggt gcatcgtgtt
    13681 gctaatgaac gtctgatgca gatgatgccg caggtattgc cttcgccaga cgcgatgggg
    13741 ccaaagttac ctgaaccggc aacggggatc gctcagccga ctccgcagca accggcgaca
    13801 ggaaatgcag ctactgcgcc tgttgcgccg acacagcccg ctgctaatcg ctctccacaa
    13861 agggctacgc cgccgcaaag tggtgctcaa ccgcctgcgc gtgcgccggg agggcaatag
    13921 tggcgagcta tcgtagccag ggacgctggg taatctggct ctctttcctc attgcgctgt
    13981 tgctgcaaat catgccctgg ccggataacc tgattgtttt ccggccaaac tgggtgttac
    14041 ttatcttgtt gtattggatc ctggccttgc cccatcgcgt aaatgtgggc acaggttttg
    14101 tgatgggtgc catactggat ctgatcagcg gctcgatgct tggcgtacgc gtattggcga
    14161 tgagcatcat tgcttacctg gtggcgctga aataccagct tttccgcaac ctcgcattat
    14221 ggcagcaggc gctggtcgtc atgttgcttt cgctggtggt ggatattatt gttttctggg
    14281 cagagttttt agtgattaac gtctctttca gaccggaagt gttctggagt agtgtagtca
    14341 atggggtgct ctggccgtgg attttcttgc tgatgcgtaa agtccgtcag cagtttgccg
    14401 tgcaataaag gtttctatga cttctctgta tttagcttcc ggttctccgc gtcgtcagga
    14461 gttacttgcg caacttggcg tgacctttga acgtattgtt acgggcattg aggagcagcg
    14521 tcagccgcag gagagcgcgc agcagtatgt tgtgcgtctg gcacgcgaga aagcacaggc
    14581 gggtgtcgcg caaacggcac aggatctccc ggtgctgggt gcggatatta tcgttatcct
    14641 gaacggagaa gtgctcgaga aaccgcgcga cgcagagcat gcggcgcaga tgttgcgcaa
    14701 attatcgggt cagacccatc aggtgatgac ggcagtggcg ttggccgaca gccagcatat
    14761 tctcgattgc ctggtggtca ccgatgtgac tttcagaacg ttaacagacg aagacatcgc
    14821 gggctatgtc gccagcggtg aaccgttaga taaagcaggt gcatacggta ttcaggggct
    14881 gggtggctgt tttgtcagga agataaatgg cagctatcac gccgtagtcg gcttaccgct
    14941 ggttgaaacg tatgaattat taagtaattt taacgcactg cgtgagaaaa gggataaaca
    15001 tgacggctga attgttagta aacgtaacgc cttcggaaac gcgagtggcg tatattgatg
    15061 gcggtattct gcaggaaatt catattgaac gtgaggcgcg acgcggaata gtaggcaata
    15121 tctacaaggg tcgtgtaagt cgtgtacttc cgggtatgca ggcggctttt gtagatattg
    15181 ggctggataa agccgcgttt cttcatgcat ccgacatcat gccgcatacc gaatgtgtgg
    15241 cgggtgaaga acaaaagcaa ttcacggtgc gcgacatctc ggaactggtt cgtcaggggc
    15301 aagatctgat ggtgcaggtg gtgaaagatc cgcttggcac taaaggtgcg cgcctgacca
    15361 ccgatatcac gctgccttct cgctatctgg tatttatgcc aggggcttct cacgttgggg
    15421 tttcccaacg aattgaaagc gaatcagaac gtgaacgcct gaaaaaagtg gtcgcagagt
    15481 attgcgacga gcagggcggg tttatcatcc gcactgcagc ggaaggggtt ggcgaggctg
    15541 aactggcctc cgatgccgct tatctgaaac gcgtctggac caaagttatg gagcgtaaaa
    15601 agcgcccgca gacccgttat cagctgtacg gcgaactggc gctggcgcag cgtgttctgc
    15661 gtgattttgc cgacgccgaa ctggaccgca ttcgcgttga ctcacgcctg acttacgaag
    15721 cattgctgga gtttacctcg gagtacattc ccgagatgac cagcaagctg gagcattaca
    15781 ccggacgcca gccgattttc gatctctttg atgtcgaaaa cgaaatccag cgagcgttgg
    15841 aacgcaaagt agaactgaaa tccggtggtt atctgattat cgaccagacc gaagcgatga
    15901 ccaccgtgga catcaatacc ggagcgtttg tcggtcatcg caatctggac gacactattt
    15961 tcaataccaa tattgaagcg acgcaggcta tcgctcgcca gttacggttg cgtaatctgg
    16021 gcgggattat cattattgat ttcatcgata tgaataatga agatcaccgc cgccgcgtgc
    16081 tgcattcgct ggagcaggcg ttgagcaaag accgggtgaa aaccagcgtt aatggttttt
    16141 cggcgctggg gctggtggag atgacgcgta aacgcacccg cgaaagcatt gagcacgtac
    16201 tgtgtaacga atgcccaacc tgccacggtc gcggaacggt gaaaaccgtg gaaacggtat
    16261 gctatgaaat catgcgcgag attgttcgtg tccaccatgc ttacgactcc gaccgtttcc
    16321 tggtctatgc ttctccggca gtagccgaag ccttgaaagg cgaagagtca cactcgctgg
    16381 cggaagtgga aattttcgtt ggcaaacagg ttaaagtaca aattgaaccg ctctataacc
    16441 aggagcagtt tgacgtcgta atgatgtaaa cagatgctgg gccgccatcc ggcaaagggt
    16501 ttttgagtca catttttagc agacaaggag tgacgggtga ggcgattgcc ggggatttta
    16561 ctgcttactg gagccgcgct cgttgtgatc gctgccctgc tggttagcgg cctgcgtatt
    16621 gctttaccgc atcttgacgc ctggcgtccg gaaatcctca acaaaataga atccgcgact
    16681 ggcatgccgg tagaagccag tcagctctca gccagctggc agaattttgg cccgacgctt
    16741 gaagcacacg atatccgtgc agaactaaaa gatggcggcg aattttcggt taaacgcgtt
    16801 actctggcgc tggatgtctg gcagagcctg ttacatatgc gctggcagtt tcgcgacctc
    16861 actttctggc agctgcgctt tcgcaccaac actcctatca ccagcggtgg tagcgatgac
    16921 agtctggaag ccagtcacat cagcgatctg tttcttcgtc aatttgacca tttcgatctc
    16981 cgcgacagtg aagtcagttt tctgacgcca tccggtcaga gcgccgagct ggcgatccca
    17041 caactcacct ggctgaacga tccacgtcga caccgtgcgg aaggcctggt aagcctctcc
    17101 agccttaccg ggcagcacgg cgtgatgcag gtgcgcatgg atttgcgcga tgatgagggg
    17161 ttgttaagca atggtcgcgt ctggctacag gcggatgaca tcgacctgaa gccgtggctc
    17221 ggtaaatgga tgcaggacaa tattgctctg gaaacggcac agttctccct tgaaggctgg
    17281 atgacgatcg acaaaggcga tgtaaccggc ggtgacgtct ggctgaaaca gggcggtgcc
    17341 agctggttgg gcgagaagca aacgcatacg ctgtcggtgg ataatctgac cgcgcatatt
    17401 acgcgtgaaa atccgggctg gcagttctct attcccgata cacggatcac gatggacggc
    17461 aaaccctggc cgagcggagc attgacgctg gcctggatac cggaacagga cgttgacggc
    17521 aaagacaata aacgcagtga cgaactccgg attcgcgcta gtaatctgga gctggcaggc
    17581 ctggagggca tacgcccgct ggccgcgaaa ctttcacctg cactgggtga tgtttggcgc
    17641 tccacacaac cgagcggcaa gattaacact ctggcgctgg atatcccgct tcaggcggca
    17701 gacaagaccc gttttcaggc atcgtggagc gatctggcct ggaagcaatg gaaattatta
    17761 ccgggtgcgg aacacttctc cgggacgctt tccggcagcg ttgaaaatgg tttgcttacc
    17821 gcgtcgatga agcaggcaaa gatgccttac gaaacggtat tccgtgcgcc actggaaatc
    17881 gccgacggcc aggcaactat aagctggctg aacaatgaca aaggtttcca gctggatggg
    17941 cgtaatattg acgttaaagc caaagccgtc catgcgcgcg gcggttttcg ttacctgcaa
    18001 cctgctaacg atgaaccctg gctgggtatt ctggctggca tcagtaccga tgatggttca
    18061 caagcctggc gctatttccc ggaaaacttg atgggtaaag acctggttga ttatttaagt
    18121 ggtgcgattc agggcggtga agcggataac gcgacgctgg tttatggtgg caatccgcaa
    18181 ctcttcccct ataaacacaa cgaaggtcag tttgaagtgc tggtgccgct gcgcaacgcg
    18241 aagtttgcct tccagccgga ctggcctgca ttaactaacc ttgatattga actggacttt
    18301 attaacgacg gtttatggat gaaaaccgat ggcgttaatc tgggcggcgt gcgcgcgagt
    18361 aatctcaccg cagtgatccc tgactactca aaagaaaaac tgctgattga cgctgacatt
    18421 aaaggtccgg gtaaagccgt tggcccttac tttgatgaga caccgctgaa agattctctg
    18481 ggtgcgaccc tgcaagaact ccagctcgac ggcgatgtga atgctcgctt acatcttgat
    18541 atcccgctga acggcgaact ggtaaccgcg aaaggtgaag tgacgctgcg taataacagt
    18601 ctgtttatca aaccactcga cagcatcctg aaaaatttga gcggtaaatt cagctttatc
    18661 aatggcgatc tgcaaagtga accactgaca gcaagctggt ttaatcagcc gttgaacgtg
    18721 gatttttcca ccaaagaagg ggcaaaagcc taccaggttg cggtgaatct caacggtaac
    18781 tggcaaccgg cgaaaaccgg cgttctgcct gaagcggtga acgaagcatt gagtggcagc
    18841 gtggcgtggg atggtaaagt gggcattgat ctgccttatc atgctggcgc gacctataac
    18901 gtagagctga acggcgatct gaataatgtg agtagtcact taccttcacc gttagccaaa
    18961 cctgcgggtg aaccactagc ggtaaacgtt aaggttgatg gcaatctcaa cagctttgaa
    19021 ttaaccggac aggctggtgc ggataaccat ttcaatagcc gctggttgct tggtcaaaag
    19081 ctgacgctcg atcgtgctat ttgggcggca gacagtaaaa cgctcccgcc gttgccggaa
    19141 caaagtggcg ttgaacttaa tatgccgccg atgaatggtg ccgagtggct ggccctgttc
    19201 cagaaaggcg ctgcggagag tgtcggtggt gcagcgagtt tcccacaaca cataacatta
    19261 cgtacgccta tgttgtcact gggaaatcag caatggaata acctgagtat tgtttcgcaa
    19321 ccgacggcaa atggcaccct ggttgaggcg caagggcgtg aaatcaacgc cacgctagcg
    19381 atgcgtaata acgcgccgtg gctggcgaat atcaaatatc tttattacaa cccgagcgtg
    19441 gcgaaagctc gtggtgattc aacgccgtca tcacctttcc cgacaacgga gcgcattaac
    19501 ttccgtggct ggccggacgc acaaatacga tgcacagagt gctggttctg ggggcaaaaa
    19561 ttcggtcgca ttgacagtga tatcaccatt tctggcgata cgttaacgct gaccaatgga
    19621 ctgattgata ctggtttctc gcggcttact gccgatggtg aatgggttaa taatccgggg
    19681 aatgaacgta cctcgctgaa aggaaaactg cgtgggcaga aaattgatgc cgccgcagaa
    19741 ttttttggtg tcacgacgcc catacgccag tcgtcattta atgtggatta cgatttacac
    19801 tggcgcaaag caccctggca gccagatgaa gcgacgttga atggcatcat tcatactcaa
    19861 ctgggtaaag gcgaaattac cgaaatcaat accggacatg ccgggcaatt gctgcgctta
    19921 ttgagcgtag atgccctgat gcgtaagctg cgttttgatt tcagagacac ttttggcgaa
    19981 gggttctatt ttgactccat tcgcagcacc gcgtggatta aagacggcgt tatgcacacc
    20041 gacgacacgc tggtggatgg cctggaggcg gatatcgcca tgaaagggtc ggtaaatctg
    20101 gtacgtcgag acctgaatat ggaagcggtt gtcgcaccag agatttctgc gacggtgggc
    20161 gtggctgcgg cttttgcggt taaccccatt gttggcgcgg cagtgtttgc cgccagtaaa
    20221 gtgctggggc cgctgtggag caaagtctcc attttgcgct atcacatttc gggtccgctg
    20281 gatgatccgc aaatcaacga agtgttgcgc caaccgcgta aagaaaaagc gcaatgattt
    20341 gacgagggcg cgtaattgcc ccaatctcat atgataatcg ttgccaaagg ccaacgagcc
    20401 ggaacataac tgtaggccgg atagggcgtt cacgccgcat ccggcagccg taaaaaatcc
    20461 tctactgcag taactaacga gtagcaaaaa cgatgagtct gaacctggta agtgaacaat
    20521 tgctagcggc gaacggcctg aaacatcagg acttgttcgc gatcctcggt caactggccg
    20581 aacgtcgcct tgattatggc gatctctatt ttcagtcgag ctatcacgaa tcctgggttt
    20641 tagaagaccg cattattaaa gatggttctt acaacatcga tcagggcgtt ggcgtgcgtg
    20701 caatcagcgg tgaaaaaacc ggatttgctt acgctgacca aatcagcctg ctggcgctgg
    20761 aacagagtgc gcaagcggcg cgcaccatcg tccgtgatag tggtgatggc aaagtacaga
    20821 cgctgggcgc ggtagagcat agcccgttgt atacctcggt agatccgctg caaagcatga
    20881 gccgtgaaga gaagctggat atcctgcgtc gcgtcgataa ggttgctcgc gaagcggaca
    20941 agcgcgtaca ggaagtgact gccagcctca gtggtgtcta tgaattaatt ctggttgcgg
    21001 ccaccgacgg cacgctagcg gcggatgtcc gtccgctggt gcgtctttcc gtgagcgttc
    21061 tggtcgaaga agatggcaaa cgcgaacgcg gtgccagtgg cggcggcggt cgttttagtt
    21121 atgagttctt ccttgccgat cttgacggcg aagtccgtgc ggatgcatgg gcaaaagaag
    21181 cagtgcgtat ggcgctggtc aatctttctg ccgttgctgc accagcgggc accatgccgg
    21241 tagtacttgg cgcaggttgg ccgggcgtgc tgttgcatga agcggtaggt cacggtctgg
    21301 aaggcgactt caaccgccgt ggcacttcag tatttagtgg acaggtcggg gagctggtgg
    21361 cttcagaact gtgtaccgtg gttgatgacg gcacgatggt cgatcgccgt ggttcggtgg
    21421 cgattgatga cgaaggtacg ccaggccagt acaacgtgct gattgagaac ggcattctga
    21481 aaggctacat gcaggataaa ctcaacgcgc gtttgatggg gatgacgccg actggcaacg
    21541 gtcgccgtga atcctacgcc catctgccca tgccgcgtat gaccaacacc tatatgctgc
    21601 cgggtaaatc gaccccgcaa gaaattattg aatccgttga gtacggtatc tatgcaccga
    21661 actttggtgg cggtcaggtg gatatcacct ccggcaaatt cgttttctcc acttcagaag
    21721 catatctgat tgaaaacggt aaagtaacga agccggtgaa aggcgcaacg ttgattggtt
    21781 ccggtatcga aaccatgcag cagatttcga tggttggcaa cgacctgaaa ctggataacg
    21841 gcgtgggtgt ctgcggtaaa gaagggcaaa gtttgccggt tggcgtgggc cagccaacgt
    21901 tgaaagtcga taacctgact gttggcggta ctgcgtaata atttcattat tttcagaagg
    21961 ataattaatc tttctacgtg cacgaacggt cccctcgctc ctctggggtt agggtgaggg
    22021 gaacccgttg gcacaggttt gtagaacgta acagtacaat atgaattact tctctttccc
    22081 gcgcccgtgc atctcctgaa acaatttacc gacctcaaca aaataatccg tcagcgagtt
    22141 gatcacgacc tgtaccttca gcggcagctt atctttttcg gtatataacg cataaaccgg
    22201 gcgtggatct gactgataac gcggcagcag gatctccagc tccccacgat tgatctcgtt
    22261 gatcacccac atcagcggca cgtaggcgat cccggcaccc gccgttagcc agcgcaccag
    22321 cgtcatcgga tcattagtca caaatctccc ttgcgggatc aggcgagtcg agatcccttc
    22381 cggtgcaatc agttcaaatt cattgtcggg ccgcacgctg tattcaagcc atgaatggct
    22441 acttaaatcg gcgggttttt ccggtatgcc gtattgtgtg agatagcttt tcgcggcgca
    22501 caccaccatt ggcatcgcgc caagacggcg ggaaaacagg ctggaatcct gcaacgcgcc
    22561 gacgcggatc accacatcca gaccgtcggc aatcaggtcg ggggctggaa ttccggtaac
    22621 cagattggcg ctcaaacctg ggtattcttt cagcattttg gctgtcagcc cggcgagaac
    22681 attttgtgcc atagttgaag aacagccaat gcgtagcgtc ccgatggggg tgttattgaa
    22741 ggcatacagt tgctcatgaa catcctgcac ttcatgaagc atacgacggc agccctggta
    22801 gtaaattcta ccggcttcgg tcaggccaat gctgcgtgtg ctacggttta acagctttac
    22861 ctgcaactca tcttccagtt ttgacaccgt ctgactgatg gacgaaacgc tcatctgtag
    22921 ctgtctggcg gcggcggtaa aagagccaaa ttcaactact ttggcaaaca ccgacatgcg
    22981 ttttagtcgt tccattattc actctgactt aaaagtgatt tagatcacat aatatagata
    23041 acagcataac agttacgcta atatattaaa tatcaatcta cagcaatgtt gctctcgccc
    23101 ggctttccat gcccttctct gcggcgacag atgctgaaaa taataacgcc tgctctctct
    23161 atacaaccaa ggtcaacatg agtctgtttc ccgttatcgt ggtgtttggg ctgtccttcc
    23221 caccgatatt ttttgaattg cttttatcac tggcgatttt ctggctggtg cgccgggtac
    23281 ttgtgccaac aggtatctac gactttgtct ggcatccggc gttgttcaac accgcgctct
    23341 attgctgctt gttttatttg atatcgcgac tgttcgtttg aggttgaagt gaaaacacta
    23401 ataagaaaat tctcccgtac ggccatcacg gtcgtattag tcattctggc cttcatcgca
    23461 atttttaatg cctgggtcta ttacaccgaa tccccctgga cgcgtgacgc gcgctttagc
    23521 gctgacgtcg ttgcgatcgc gccggacgtt tctggactca ttacccaggt gaatgttcat
    23581 gataaccagc tggtgaaaaa aggacagata ctgttcacca tcgaccagcc gcgctatcaa
    23641 aaggcgcttg aggaagcgca agccgatgtt gcttattatc aggtactggc acaggagaaa
    23701 cgccaggagg ccggacgtcg taaccgtctc ggtgtgcagg cgatgtctcg cgaagagatc
    23761 gaccaggcta acaacgtact acaaacggtt ctgcatcagt tagcgaaagc gcaggcgacc
    23821 cgcgatctgg caaaactgga tcttgaacgc acggtgatcc gagcgccagc agatggctgg
    23881 gtgaccaacc tcaacgtcta taccggtgag tttattactc gaggatcaac ggcggttgcg
    23941 ctggtgaaac agaactcctt ctatgtactg gcctatatgg aagaaactaa gctggaaggg
    24001 gtgcgtccgg ggtatcgtgc agagatcacg ccgcttggca gtaacaaagt gctgaaaggg
    24061 actgttgata gtgttgccgc tggggtcacc aacgccagca gcacgcgtga cgacaaaggg
    24121 atggcgacca tcgactctaa ccttgaatgg gtgcgtttgg cgcaacgtgt tccggttcgt
    24181 attcgtctcg acaaccagca agagaacatc tggcctgcgg gcaccactgc tacagtggtg
    24241 gtcactggca aacaagatcg cgacgaaagc caggattcgt tcttccgtaa aatggcccat
    24301 cgcctgcgtg agtttggtta atcacgatgg gtattttctc cattgctaac caacatattc
    24361 gctttgcggt aaaactggcg accgccattg tactggcgct gtttgttggc tttcacttcc
    24421 agctggaaac gccacgctgg gcggtactga cagcggcgat tgttgccgcc ggtccggcct
    24481 ttgctgcggg aggtgaaccg tattctggcg ctattcgcta tcgtggcttt ttgcgcatca
    24541 tcggcacatt tattggctgt attgccggac tggtgatcat cattgcgatg atccgcgcac
    24601 cattattgat gattctggtg tgctgtatct gggccggttt ttgtacctgg atatcctcgc
    24661 tggtacgaat agaaaactcg tacgcgtggg ggctggccgg ttataccgcg ctgatcattg
    24721 tgatcaccat tcagccggaa ccattgctta cgccgcagtt tgccgtcgaa cgttgtagcg
    24781 agatcgttat cggtattgtg tgtgcgatta tggcggattt gctcttttct ccgcgatcga
    24841 tcaaacaaga agtagatcga gagctggaaa gtttgctggt cgcgcaatat caattaatgc
    24901 aactctgtat caagcatggc gatggtgaag ttgtcgataa agcctggggc gacctggtgc
    24961 gacgcaccac ggcactacaa ggcatgcgca gcaacctgaa tatggaatct tcccgctggg
    25021 cgcgggccaa tcgacgttta aaagcgatca atacgctatc gctgacgctg attacccaat
    25081 cctgcgaaac ttatcttatt cagaatacgc gcccggaatt gatcactgat actttccgcg
    25141 aattttttga cacgccggta gaaaccgcgc aggacgtcca caagcagctc aaacgcctgc
    25201 ggagagttat cgcctggacc ggggaacggg aaacgcctgt caccatttat agctgggtcg
    25261 cggcggcaac gcgttatcag cttctcaagc gcggcgttat cagtaacaca aaaatcaacg
    25321 ccaccgaaga agagatcctg caaggcgaac cggaagtaaa agtagagtca gccgaacgtc
    25381 atcatgcaat ggttaacttc tggcgaacca cactttcctg cattctgggc acgcttttct
    25441 ggctgtggac gggctggact tccggcagtg gtgcaatggt gatgattgcg gtcgtgacgt
    25501 cactggcaat gcgtttgccg aatccacgca tggtggcgat cgactttatc tatgggacgc
    25561 tggccgcgct gccgttaggg ctgctctact ttttggtgat tatccctaat acccaacaga
    25621 gcatgttgct gctgtgtatt agcctggcag tgctgggatt cttcctcggt atagaagtac
    25681 agaaacggcg actgggctcg atgggggcac tggccagcac cataaatatt atcgtgctgg
    25741 ataacccgat gactttccat ttcagtcagt ttctcgacag cgcattaggg caaatcgtcg
    25801 gctgtgtgct cgcgttcacc gttattttgc tggtgcggga taaatcgcgc gacaggactg
    25861 gacgtgtact gcttaatcag tttgtttctg ccgctgtttc cgcgatgact accaatgtgg
    25921 cacgtcgtaa agagaaccac ctcccggcac tttatcagca gctgtttttg ctgatgaata
    25981 agttcccagg ggatttgccg aaatttcgcc tggcgctgac gatgattatc gcgcaccagc
    26041 gcctgcgtga tgcgccgatc ccggttaacg aggatttatc ggcgtttcac cgacaaatgc
    26101 gccgcacagc agaccatgtg atatctgccc gtagcgatga taaacgtcgt cggtactttg
    26161 gccagttgct ggaagaactg gaaatctacc aggaaaagct acgcatctgg caagcgccac
    26221 cgcaggtgac ggaaccggtt catcggctgg cggggatgct ccataagtat caacatgcgt
    26281 tgaccgatag ttaagtcaaa accgacgcca aaagcgtcgg ttttttcatg gctatactta
    26341 gcgatgcacg gcagaactcg ccgcgaaacg tgacggtggc aacagatgaa tatttatgcc
    26401 tttgatttta atgagattga gagtcaggag gatttttatc gtgactttag ccaaaccctt
    26461 ggtctggcta aagataaggt acgcgatctt gactcactat gggatgtgtt aatgaacgat
    26521 gtcctgccgc taccacttga gattgaattt gttcatctgg gagagaaaac gcgtcgccgt
    26581 tttggcgcgt taatattgct gtttgatgag gcagaggaag agctggaagg gcatttgcgt
    26641 tttaatgttc gtcattagcg caaaaaaaag cccccgaacc gggggcaata tcgtcggaca
    26701 aaacgatgag ggtttatttg tacagttcag ccgtagcgtg ccaggtgtca ccgctacgag
    26761 cttcagtaat ctggtaggcc gttgcgcctt tctcttccgc ttttttgttc agcatttcac
    26821 gtatatccat tggcgaagac gccacaccac ttacggatac ggtcccgatt gcttcacgat
    26881 tttgtgcttg tgcagcatca atggagtcgg cagcgaatgc accgaaagag agaacagaaa
    26941 gtacgcttaa tgcagctaca gtggttttga ttttcatgat ttttacctcg acataatctt
    27001 ttagctgggc ctttgtttcg tgacccatat cacaaaatca agcatacact aattattgaa
    27061 ctaattaata ccacgctaac tgtttcagtt gatactaagt gttaaaaatg tgaattttaa
    27121 taacaaaaaa gtataaaaaa cacggttatt tattattaat tatagtattt tcaattagat
    27181 aatgaatttt gcataatgtg ctttttttac cgtatgtatt cattttgtga atgatgtgtc
    27241 gcggtaaaac gcactattcg ttaaatgtat cgcaggagaa agaaggggaa tgagagggat
    27301 aagcaacatt ttccccgccg ccagaagcga cggggcagag attaaagctc ctggtcgaac
    27361 agctctaaaa tcgcttcgta caggtctttg acggtgaaac cgttagcagg ggtggtaaag
    27421 atggtgtcat cgccagcgat ggtgcccaga ataccttctg ctttgcccag tgagtccagc
    27481 aggcgagcaa ttaactgcgc cgcgccaggg ctggtatgaa tcacgacaac tgcatcgttg
    27541 tagtcgatat ccagcaccag attcttcaat ggactggagg tggttggtac acccagttca
    27601 gctggcaggc agtaaaccat ttccattttg gcattgcgtg tacgtacagc accaaacttg
    27661 gtcaacatcc gcgagacttt agactgatta atattgtcaa agccttgctc ctgcaacgcg
    27721 gcgacgattt cgccctggga gctaaatttc tcttctttaa gtaatgcttt aaatgcttta
    27781 actagttctt cttgcttagc cgagcttcgc ataagtcacc cgatatggtg gttgatacaa
    27841 cattattgtg catacagatg aatttttatg caaacagtca gccctgaaga aggctgaaat
    27901 aatgttatga aagagcggga ttttatcaaa tttcgttatt gagaaacatg cctgcgtcac
    27961 ggcatgcaaa ttctgtttaa aagtaaatta attgttatca aattgatgtt gttttggctg
    28021 aacggtaggg tatattgtca ccacctgttg gaatgttgcg ctaatgcata agcgactgtt
    28081 aattacgtaa gttaggttcc tgattacggc aattaaatgc ataaacgcta aacttgcgtg
    28141 actacacatt cttgagatgt ggtcattgta aacggcaatt ttgtggatta aggtcgcggc
    28201 agcggagcaa catatcttag tttatcaata taataaggag tttaggatga aagtcgcagt
    28261 cctcggcgct gctggcggta ttggccaggc gcttgcacta ctgttaaaaa cccaactgcc
    28321 ttcaggttca gaactctctc tgtatgatat cgctccagtg actcccggtg tggccgtcga
    28381 tctgagccat atccctactg ctgtgaaaat caaaggtttt tctggtgaag atgcgactcc
    28441 ggcgctggaa ggcgcagatg tcgttcttat ctctgcaggc gtagcgcgta aaccgggtat
    28501 ggatcgttcc gacctgttta acgttaacgc cggcatcgtg aaaaacctgg tacagcaagt
    28561 tgcgaaaaac tgcccgaaag cgtgcattgg tattatcact aacccggtta acaccacagt
    28621 tgcaattgct gctgaagtgc tgaaaaaagc cggtgtttat gacaaaaaca aactgttcgg
    28681 cgttaccacg ctggatatca ttcgttccaa cacctttgtt gcggaactga aaggcaaaca
    28741 gccaggcgaa gttgaagtgc cggttattgg cggtcactct ggtgttacca ttctgccgct
    28801 gctgtcacag gttcctggcg ttagttttac cgagcaggaa gtggctgatc tgaccaaacg
    28861 tatccagaac gcgggtactg aggtggttga agcgaaagcc ggtggcgggt ctgcaaccct
    28921 gtctatgggc caggcagctg cacgttttgg tctgtctctg gttcgtgcac tgcagggcga
    28981 acaaggcgtt gtcgaatgtg cctacgttga aggcgacggt cagtacgccc gtttcttctc
    29041 tcaaccgctg ctgctgggta aaaacggcgt ggaagagcgt aaatctatcg gtaccctgag
    29101 cgcatttgaa cagaacgcgc tggaaggtat gctggatacg ctcaagaaag atatcgccct
    29161 gggcgaagag ttcgttaata agtaattgat tagcgaataa taaaaaaccg gagcacagac
    29221 tccggttttt gttttgagca ctcgacttaa ttggttgccg gatattcctg aatggtgacc
    29281 tgcagcgtta actgcttatc atcacgcatc actacaaccg ggatcaccga accagggcga
    29341 atttccgcca cctgatccat cgtctccaga gcagagatgg ccggtttgtt atccaccgaa
    29401 ataatcagat cgttgacctg aatacccgca ttcgccgctg ggccgtcagg tgacacttca
    29461 ttaaccacga tcccttgcag ttgatctata ccaccgctct gcgcgtgcag tggtgcgatc
    29521 tcgcgtccgc cgataccaat gtagccgcgg atcacgcgac catcgcggat cagcttatcc
    29581 ataattttgg ttgctaactg gaaaggaatc gcaaagccga taccttccgg cgtttcgcca
    29641 tcgttactct tatcaaacga cagcgtgtta atgcccatca gttctcccag cgagttcacc
    29701 agcgcgccgc cagagttacc gtggttaatg gaagcatcgg tttgtaggaa gttttgccgc
    29761 ccggtcgggt tcagaccgat tcgacccgtg gcactaataa tcccttgggt aatggtctgc
    29821 ccgaggttgt acgggttacc gatcgccagt actacgtcgc caatgtgcgg tacgcgacgt
    29881 gcattaattg gaatggtagg taaaccgcca gtggcattaa ttttaagtac cgccagatcg
    29941 gttagagagt cagatcccac cagcaatgct tcaaatacac gtccatcctg taaggcgacg
    30001 atgatctgat cggcgtcgtt gatgacgtgt ttattggtga tgatataacc gcgttgatcc
    30061 atgattacac cggatcccag ggtgcggatc tcaagctggt tgtgagagtt ggtgttcaaa
    30121 ccacggttgt aaacgttaac caccgctggc gcggcgcggc gaaccgccag attatagctg
    30181 gcaggcgtct catcggtact gtcaaattgc ggagtggaaa gcgggttaag gctgcgcagc
    30241 gaaggcatgg caaccagcag aatagcgccg acaattaatc caatcgcaac ggaacgtaag
    30301 agcttcacaa acatgatgga ggcgtcatta aaaaagggaa cggcagcagc ataccacgag
    30361 ttaaccggac atcacacgtc agcctgatgc ccggtttacg acattaacgc atcagcagat
    30421 agatgctttc attgccgcgt acaatttgca gggcgatgat ggccggtttt gccgccagca
    30481 ctttacgcat ttcagcaatc gagttcaccc gatcgcggtt gacgccaatg atcacatcgt
    30541 ctttttgcaa gccagcctga gcagctgggc ttcctttgac aacttcatct atcttaatac
    30601 ctttgccgcc atcttttagc tgaccatcgc tcaacgttgc accttccagc gctggcgtga
    30661 tcatttcagc gctggccgac gaagaggtgt tggtatcaag cgtcacttct acttccagtg
    30721 gtttgccgtt acgcagcagg ccaagcttca ctttcgtgcc cggctctgta gtcgcgatac
    30781 gagagcgcaa ctcagcaaag ctattcagcg gtttgccgtt gaggctggta ataatatcgc
    30841 ccgctttgac gcccgctttc gctgagccag aacttggcaa cacttcgctg acaaacgcgc
    30901 cacgctgcac gtcaaggttg aaggctttgg cgatatcggc actcatctcg gtgcctttga
    30961 tgcctaacaa accgcgtttg atttcaccaa agtcgataag ctgctgcgcc agtgttcgcg
    31021 ccatattact ggggatggca aatccaatcc cgacgctccc gccgccaggc gcaaggattg
    31081 cagtgttgat gccaattaac tcaccgttaa ggtttaatag tgcaccgccg gagttaccgc
    31141 ggttaatgga agcatctgtc tggataaagt tttccagacc ttcaagattc aacccgctgc
    31201 ggcctaatgc ggaaacaatg ccagaggtgg cggtttgccc aaggccaaat gggttaccga
    31261 ccgctacggc aaaatcaccg acgcgcaatt tatcggagtc ggcaatagcg atttgcgtta
    31321 atttgctcgg gttttgaatt tgtaacaggg cgatatcgct ctggtcatcg ctaccaatca
    31381 gtttttcatc aaactcgcgc ccatcattga gctgaatact gattttctgt gcctgattaa
    31441 tcacatggtt gttggtcagc acatagcctt tactggcgtt gatgatgaca ccggaaccta
    31501 aaccttcgaa gggttgtgca ggttgatccg gtaaatcatc accaaaaaac tttttgaatt
    31561 cttccgggat tttctgtccc tgactggccg ttccttccac ccgtacgctc accactgccg
    31621 gaagcacttt ttccagcatt ggagccagac tggggagagg ggcctgatcg gcaacctggc
    31681 ctggaatcga cgcgacggcc tgaaatgacg ccgagagagt taacccggca cttaacgcta
    31741 atgcactcaa cagctgggtt tgttttttca ttattcctgc tctcgtacct gaatgataag
    31801 aaaagagatt caaaacagtt tgatgttatt gaattttcag acggctaaca atgagacacc
    31861 agattaaatg atagtcgtgg agggagagaa gaggagggcg caatggctgc gcccgaaaaa
    31921 taaattagtc gcgcttcgcg ccagtacgca gcaggccgga tgcgccttca gaatagtcgc
    31981 gaggcatctg caccggtgcc tgatcgttgc tggcttcaga ctctgccaga cgattacgga
    32041 acgggtttgc ttcagcagac agttccggca gcaggctgct ggagcttttt gccatgtgct
    32101 gatacagctg gcgatagtcg tgcgccatgg tatccagtaa ttccgcgctg cgggcaaagt
    32161 ggctaaccag ctcttcgcga tactcgtcca gttcagcttt attcttttcc agttcgtact
    32221 gcaacgcctg ttgctggcgt agtttacgat taccaaaacg catggccaca gcaccaataa
    32281 tgatgccgac gactaaccca attagcgcat attcccaggt catgaacatc tcccgttgtc
    32341 ttttgtttcc gtagggtgtt ggcttcaggc tccatgcctg cggctgatta tgccactata
    32401 accgttaatt ccacagaagt ggaatcccga ctgcatatcg cgtagtgtag aacggccttt
    32461 ttttcgtcaa ccgtgaacaa cggcgcaccg attattcaag gaataacaat aagatcatgc
    32521 aaagcgttac cccaacatcg caatacctga aggcgctcaa tgaaggcagc catcaacccg
    32581 acgacgttca aaaagaggcc gtcagccgcc tggaaattat ttatcaagaa ctcatcaata
    32641 gcaggccacc agcccccagg acgagtgggc taatggcgcg ggtcggtaag ctgtggggta
    32701 aacgcgaaga cacaaagcat acgccagtgc gtggcttata tatgtggggc ggtgtaggac
    32761 gcgggaaaac ctggctgatg gaccttttct atcaaagcct gccgggagag cggaaacagc
    32821 gcctgcactt tcaccgtttt atgctgcggg tgcacgaaga gctaactgcc ttacaggggc
    32881 agaccgatcc gctggaaatt attgccgatc gctttaaagc cgaaactgac gtgctctgtt
    32941 ttgacgaatt ttttgtttct gatattaccg acgccatgct acttggcggt ctgatgaaag
    33001 ccctgttcac ccgcggtatt accctggtag cgacgtcaaa tattccgccg gacgaacttt
    33061 atcgaaatgg cctgcaacgt gcgcgttttc tgcctgcaat cgatgccatt aaacagcatt
    33121 gtgatgtaat gaacgtggac gctggtgttg attatcgact gcgtacactc actcaggcgc
    33181 atctgtggct ttcgccactc aacgatgaaa cccgggcgca gatggataaa ctatggttgg
    33241 cgctggcggg ggtgaaacga gaaaattcac cgacgttaga aatcaaccat cggccattgg
    33301 cgacaatggg cgtcgagaac cagacgctgg cggtttcttt tactacgctg tgcgtcgacg
    33361 cccgcagtca gcatgactat attgcgctct cacgcctgtt tcacacagta atgttgtttg
    33421 atgtgccagt catgacgcgg ttgatggaga gcgaagcgcg gcgctttatt gcgctggtgg
    33481 atgagtttta cgagcgccat gtcaaattag tggtgagtgc agaagtgccg ctgtatgaaa
    33541 tttatcaggg tgagcggctg aagtttgagt tccagcgttg cctgtcacgt ctgcaagaga
    33601 tgcaaagcga agagtatctg aagcgcgagc atttggcggg ttaaaacctg ccacaaatca
    33661 caaaaagggg tcgatctttg accccgactt ctctataatc ctgcgacccc acgttacaag
    33721 aaagtttttt tcccaaaact ttttgtgtgc tggcataggc tattcgaagg ggtaggtttg
    33781 ccggactttg tcgtgtgaac ctcaacaatt gaagacgttt gggtgttcac caacgtgtaa
    33841 ctatttattg ggtaagcttt taatgaaaac ttttacagct aaaccagaaa ccgtaaaacg
    33901 cgactggtat gttgttgacg cgaccggtaa aactctgggc cgtctggcta ctgaactggc
    33961 tcgtcgcctg cgtggtaagc acaaagcgga atatactccg cacgtagata ccggtgatta
    34021 catcatcgtt ctgaacgctg acaaagttgc tgtaaccggc aacaagcgta ctgacaaagt
    34081 gtactatcac cacaccggcc acatcggtgg tatcaaacaa gcgacctttg aagagatgat
    34141 tgctcgccgt cctgagcgtg tgattgaaat cgcggttaaa ggcatgttgc caaaaggccc
    34201 gctgggtcgt gctatgttcc gtaaactgaa agtttacgcg ggtaacgagc acaaccacgc
    34261 ggcacagcaa ccgcaagttc ttgacatcta atcgggatta taggcaatgg ctgaaaatca
    34321 atactacggc actggtcgcc gcaaaagttc cgcagctcgc gttttcatca aaccgggcaa
    34381 cggtaaaatc gtaatcaacc aacgttctct ggaacagtac ttcggtcgtg aaactgcccg
    34441 catggtagtt cgtcagccgc tggaactggt cgacatggtt gagaaactgg acctgtacat
    34501 caccgttaaa ggtggtggta tctctggtca ggctggtgcg atccgtcacg gtatcacccg
    34561 cgctctgatg gaatacgacg agtccctgcg ttctgaactg cgtaaagctg gcttcgttac
    34621 tcgtgacgct cgtcaggttg aacgtaagaa agtcggtctg cgtaaagcac gtcgtcgtcc
    34681 gcagttctcc aaacgttaat tggcttctgc tccggcagaa aacaatttgc gaaaaaaccc
    34741 gcttcggcgg gtttttttat ggctaaattc tgaatcagcg taaaaaatgg gaagttgctt
    34801 tttgctgccg tctgaaagac aggtaaaaca aaaaccacat cgccaataag ggtctaagtc
    34861 aactatttca gactaaagcg catctctttt tccccatttc cggcatccac tcaccacaaa
    34921 ggtcgcaaaa tctggtaaac tatcatccaa ttttctgccc aaatgtcggg tattgctcat
    34981 tttttgtttg attttcgaac aaagagagtc gttccttatt gggtaacaca acttctgact
    35041 ggccacctgg tggctggtag cagtaaaaat tctgactata cctggaggtt ttcatggctg
    35101 tcgctgccaa caaacgttcg gtaatgacgc tgttttccgg tcctactgac atctatagcc
    35161 atcaggtccg cattgcgccg cgtgctgtcg gcaatctgga actggcgaat gatgaggtgc
    35221 gctttaacgc gcgctttggt ggcattccgc gtcaggtttc tgtgccgctg gctgccgtgc
    35281 tggctatcta cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa gctgcctacg
    35341 atgaagatac cagcatcatg aatgatgaag aggcatcggc agacaacgaa accgttatgt
    35401 cggttattga tggcgacaag ccagatcacg atgatgacac tcatcctgac gatgaacctc
    35461 cgcagccacc acgcggtggt cgaccggcat tacgcgttgt gaagtaatac aaaacaggcc
    35521 caggcggcct gttttgtctt tttaatgact taataaaata gcgtgattgt ggcaatcata
    35581 ttcaccacga aaccgactgc catggggatc gctgtccgct tcaccaccgc aaaaggcgaa
    35641 acgcctgcaa ttcctgacgt aacgaccacc accgcagtaa tcggcgaaac cgcgcgcgct
    35701 agcgtcgtgg caaaatgcat cggcataatc attacaaccg ctggtacatg tagtccggct
    35761 gcgatattcg gaataagact ggcaaatgac ataaacggcg cattgccaga gcccatcaca
    35821 atggcacaaa tggcaatgac tagcgccata ataatcatca cgccaatacc gcccagacca
    35881 gaatgctccg cacccctgat aaccgcatca acagtgccaa tcgtcgttaa gcctttcgca
    35941 aatatttcac ccgcgaccac cagcgttacc acgttgacaa actgcgtacc catgccgtca
    36001 aaaaacgcct gcacatcgtc cattgtttcg cgcaagttat gcttgcggaa gaactcaaca
    36061 aacatcgtca ccgtcaaact tagtaacatc accaccacca gatgcagttc tgacttcatc
    36121 agcccgacgt gggcgaggaa cagcgagccg agcatcagga ttaacggcat cacaggtaaa
    36181 atggcgtaat agagcggcgg gacattatcg agagtttttt gctctgcctg ttcgtgattg
    36241 atatttttat cttttttgtc aaaagcgcgt tgcacgaaaa agtgggagat cgccaccgag
    36301 ataatgacgc aagaggcgac cggaagctgg tagtggaaga agtatgtggc aattttcatt
    36361 cccgcgacct gggctgcaaa aatggagttc gtttccagaa tcccccactc aatggacatc
    36421 gtggttgcga taaccgccac tgcagagagg cgacttactc ccagactcac cagcgtcgga
    36481 aataaggtga ccatcagcaa catacccaga ccggaggcgc tggtaataaa ctgcgccatg
    36541 atttggccga tgacgtaagt tgccgacaga ataatatacg gcgagcgaat gagttttaac
    36601 gggcggctta gcaagctcac catcgcgcga ctggctccta tgcgctccat gtagcgggca
    36661 taaccgccca ccgccataat cgacagcccc aatccggcaa tgcggttgga cagcatgcgc
    36721 aggatttcat tataaatgtc gacgatcaag tagccgctgc ttttggcggg atcgagtaca
    36781 ccaccgaacc ctaaccagac tccgcacatc atcaggaaga cacctgcggc agccagcacc
    36841 acctgaggtt tgtagttttt caggatcaaa tagcccatcg taattaatac gatgacagat
    36901 ataattatgc cgaacatt
//
LOCUS       DADMGC010000022        36607 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.022, whole genome shotgun sequence.
ACCESSION   DADMGC010000022 DADMGC010000000
VERSION     DADMGC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.022"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            27..2294
                     /gene="adiA"
                     /locus_tag="JNZ73_001132"
     CDS             27..2294
                     /gene="adiA"
                     /locus_tag="JNZ73_001132"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAY8731489.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     INEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVIHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            2619..3380
                     /locus_tag="JNZ73_001133"
     CDS             2619..3380
                     /locus_tag="JNZ73_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8731490.1"
                     /translation="MRICSDQPCIVLLTEKDVRIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            3517..4854
                     /gene="adiC"
                     /locus_tag="JNZ73_001134"
     CDS             3517..4854
                     /gene="adiC"
                     /locus_tag="JNZ73_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HAY8731491.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            4958..6601
                     /gene="eptA"
                     /locus_tag="JNZ73_001135"
     CDS             4958..6601
                     /gene="eptA"
                     /locus_tag="JNZ73_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HAY8731492.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRTLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            6598..7266
                     /gene="basR"
                     /locus_tag="JNZ73_001136"
     CDS             6598..7266
                     /gene="basR"
                     /locus_tag="JNZ73_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HAY8731493.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            7267..8367
                     /gene="pmrB"
                     /locus_tag="JNZ73_001137"
     CDS             7267..8367
                     /gene="pmrB"
                     /locus_tag="JNZ73_001137"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HAY8731494.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="JNZ73_001138"
     CDS             complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="JNZ73_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HAY8731495.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(8544..10046)
                     /gene="proP"
                     /locus_tag="JNZ73_001139"
     CDS             complement(8544..10046)
                     /gene="proP"
                     /locus_tag="JNZ73_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HAY8731496.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(10310..11188)
                     /locus_tag="JNZ73_001140"
     CDS             complement(10310..11188)
                     /locus_tag="JNZ73_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731497.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNSGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="JNZ73_001141"
     CDS             complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="JNZ73_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HAY8731498.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            13815..14150
                     /locus_tag="JNZ73_001142"
     CDS             13815..14150
                     /locus_tag="JNZ73_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HAY8731499.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            14308..14751
                     /gene="yjdN"
                     /locus_tag="JNZ73_001143"
     CDS             14308..14751
                     /gene="yjdN"
                     /locus_tag="JNZ73_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HAY8731500.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            14884..15672
                     /gene="phnC"
                     /locus_tag="JNZ73_001144"
     CDS             14884..15672
                     /gene="phnC"
                     /locus_tag="JNZ73_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAY8731501.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            15697..16713
                     /gene="phnD"
                     /locus_tag="JNZ73_001145"
     CDS             15697..16713
                     /gene="phnD"
                     /locus_tag="JNZ73_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8731502.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSSGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            16819..17598
                     /gene="phnE"
                     /locus_tag="JNZ73_001146"
     CDS             16819..17598
                     /gene="phnE"
                     /locus_tag="JNZ73_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAY8731503.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAEEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            17619..18344
                     /gene="phnF"
                     /locus_tag="JNZ73_001147"
     CDS             17619..18344
                     /gene="phnF"
                     /locus_tag="JNZ73_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HAY8731504.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            18345..18797
                     /gene="phnG"
                     /locus_tag="JNZ73_001148"
     CDS             18345..18797
                     /gene="phnG"
                     /locus_tag="JNZ73_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HAY8731505.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHTERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMIRGDNA"
     gene            18794..19378
                     /gene="phnH"
                     /locus_tag="JNZ73_001149"
     CDS             18794..19378
                     /gene="phnH"
                     /locus_tag="JNZ73_001149"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HAY8731506.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAISEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SKQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            19378..20442
                     /gene="phnI"
                     /locus_tag="JNZ73_001150"
     CDS             19378..20442
                     /gene="phnI"
                     /locus_tag="JNZ73_001150"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HAY8731507.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGVADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            20435..21280
                     /gene="phnJ"
                     /locus_tag="JNZ73_001151"
     CDS             20435..21280
                     /gene="phnJ"
                     /locus_tag="JNZ73_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HAY8731508.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            21277..22035
                     /gene="phnK"
                     /locus_tag="JNZ73_001152"
     CDS             21277..22035
                     /gene="phnK"
                     /locus_tag="JNZ73_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HAY8731509.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            22146..22826
                     /gene="phnL"
                     /locus_tag="JNZ73_001153"
     CDS             22146..22826
                     /gene="phnL"
                     /locus_tag="JNZ73_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HAY8731510.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            22823..23959
                     /gene="phnM"
                     /locus_tag="JNZ73_001154"
     CDS             22823..23959
                     /gene="phnM"
                     /locus_tag="JNZ73_001154"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HAY8731511.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLLLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHLLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     SGSHSGNVAASELAQLGLLDILSSDYYPTSLLDAAFRVADDQSNRFTLPQAVKLVTQN
                     PAQALNLQDRGVIGEGKRADLVLAHRKDNHIHIDHVWRQGKRVF"
     gene            23959..24516
                     /gene="phnN"
                     /locus_tag="JNZ73_001155"
     CDS             23959..24516
                     /gene="phnN"
                     /locus_tag="JNZ73_001155"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HAY8731512.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAEIRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANSLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQALARYQSALLPICLLVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            24503..24937
                     /gene="phnO"
                     /locus_tag="JNZ73_001156"
     CDS             24503..24937
                     /gene="phnO"
                     /locus_tag="JNZ73_001156"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HAY8731513.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVSMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTALSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            24939..25697
                     /gene="phnP"
                     /locus_tag="JNZ73_001157"
     CDS             24939..25697
                     /gene="phnP"
                     /locus_tag="JNZ73_001157"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HAY8731514.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRAQRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHAQGLFPLRWGVGDPIPIYGPP
                     DDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLIENALPSGFEAGFDGMEIGVA"
     gene            complement(25844..26173)
                     /locus_tag="JNZ73_001158"
     CDS             complement(25844..26173)
                     /locus_tag="JNZ73_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731515.1"
                     /translation="MKPYSTLFLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNSDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRCQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(26242..26874)
                     /locus_tag="JNZ73_001159"
     CDS             complement(26242..26874)
                     /locus_tag="JNZ73_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8731516.1"
                     /translation="MIVMPTTYSPMTVARSFRVVEEIELSGRLLQVIYDNQTPRAAII
                     EADIEVFAGVPRHRVLAALDLQRKKEFGSDIIAVERWWEDSDLVQVEGICVSPGERDK
                     GLATRLYEALILQCGITLISDFDQYDGGKMLWQKIARESDQISVFVLDTEQGAFWPYD
                     GTKVIYDGCCIPEERIWSISPDQKCHGIVLVAENKQRANQLMEESAPYRI"
     gene            complement(26955..29170)
                     /locus_tag="JNZ73_001160"
                     /pseudo
     CDS             complement(26955..29170)
                     /locus_tag="JNZ73_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
     gene            29284..30804
                     /locus_tag="JNZ73_001161"
     CDS             29284..30804
                     /locus_tag="JNZ73_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY8731517.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSCL
                     QVLSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAVENITMATLERDASWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVHVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            30801..31775
                     /locus_tag="JNZ73_001162"
     CDS             30801..31775
                     /locus_tag="JNZ73_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY8731518.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVLCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSTSRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            31806..32747
                     /locus_tag="JNZ73_001163"
     CDS             31806..32747
                     /locus_tag="JNZ73_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY8731519.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKDLKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNKNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     ISYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            32802..33485
                     /locus_tag="JNZ73_001164"
     CDS             32802..33485
                     /locus_tag="JNZ73_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAY8731520.1"
                     /translation="MKRSAINNILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     VVFDLKLGWDVTAFGGNNFAAQRLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTVDTDVTVVIDGCRQTHVAGSQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFL"
     gene            33500..34360
                     /locus_tag="JNZ73_001165"
     CDS             33500..34360
                     /locus_tag="JNZ73_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HAY8731521.1"
                     /translation="MPLISLAEGLAHAREHHYALGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARQAIPVVLNLDHGLHFESVAQALRLGFSSVM
                     FDGSTLGYEENIRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGIRDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            34357..35358
                     /locus_tag="JNZ73_001166"
     CDS             34357..35358
                     /locus_tag="JNZ73_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HAY8731522.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLLLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQCGVWIPSQKLEQREIIGS
                     VGAGDVFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(35355..36061)
                     /locus_tag="JNZ73_001167"
                     /pseudo
     CDS             complement(35355..36061)
                     /locus_tag="JNZ73_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            36352..>36607
                     /locus_tag="JNZ73_001168"
     CDS             36352..>36607
                     /locus_tag="JNZ73_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731523.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYY"
BASE COUNT         8529 a        10227 c         9717 g         8134 t
ORIGIN      
        1 attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc gagtttctcc
       61 atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta agccagcaaa
      121 atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc tcttcaaacg
      181 aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa catcaaaacg
      241 tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc ttcctgttgg
      301 gcgatcggga aaaagccctc gccgcaatgg accgggatct gctggagctt gtcgatgaat
      361 tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgccgtt gccgcgatga
      421 cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa tatagtgaca
      481 tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt accaaaacac
      541 ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc gacatgggca
      601 tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc gaaagcgaaa
      661 aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc ggtacttccg
      721 gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg gtcgttgacc
      781 gtaactgcca taaatccatc gaacaaggtt tgatgctgac aggcgcgaaa ccggtctata
      841 tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag gaaatgcaac
      901 ctgaaacctt gcaaaagaaa atcagtgaaa gcccgctgac caaagacaaa gccgggcaaa
      961 aaccgtctta ctgcgtggta accaactgca cctatgacgg cgtgtgttat aacgctaaag
     1021 aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa gcctggtacg
     1081 gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc gaacctggcg
     1141 atcacaacgg tcctaccgtt ttcgccaccc actccatcca caaactgctg aatgcgctgt
     1201 cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc tcccgcttca
     1261 accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc gcatccaacg
     1321 acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacacag gaagtgatta
     1381 acgaagcggt tgatttccgt caggcgatgg cgcggctata taaagagttc accgctgacg
     1441 gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa accggcaaaa
     1501 cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac tgctgggtaa
     1561 tacatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg agtatgctcg
     1621 acccaattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa ctggaagaaa
     1681 ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt gtgcctaccc
     1741 gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt gggaaatggg
     1801 ggactctggt taacaccctt tgctccttca aacgccacta tgacgccaac acaccgctgg
     1861 cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac atggggattc
     1921 acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc gcacggttga
     1981 acgaagccta ttccggcctg ccggtggcgg aaatcacccc gcgtgaagcg tacaacgcga
     2041 ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc atcgcggcaa
     2101 actcagttat cccgtatccg ccaggaatcc cgatgctgct gtctggtgaa aacttcggcg
     2161 ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac caccatttcc
     2221 ctggatttga acacgaaact gaagggactg aaattattga cggtatttac cacgttatgt
     2281 gcgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac attacgccgc
     2341 ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca aacaaaaatg
     2401 tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta tcctgtaaaa
     2461 aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact cgttattttt
     2521 atgcatgttt atattcatca tataattata taaccatttc ccggtatcgc tttgctttag
     2581 cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc agcgaccaac
     2641 cttgtattgt tttattgact gaaaaagatg tccggataag ggtgaatggg aaagaaccta
     2701 ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt atcgacgtct
     2761 cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa gactacctcc
     2821 ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct acgcccatac
     2881 tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac agcatgatgc
     2941 ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg gtgctatccc
     3001 gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt aattgtgtaa
     3061 gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat cttagtatgg
     3121 tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagttgaaa agcgaaaaca
     3181 ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat gaattaatga
     3241 tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt acgtcgtact
     3301 ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt agtcagcaca
     3361 gagaacgcac tgtcgcctga tttttaacct taacgaagag ctatattaat aacggcatca
     3421 gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata tgacgtaatt
     3481 tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc tgatgctcac
     3541 aaagtgggct taatccccgt caccctgatg gtgtcgggga atattatggg gtcaggtgtt
     3601 tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg atggttggtg
     3661 acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt cctcgaccca
     3721 agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct cggttatcaa
     3781 accaacgtcc tctactggct ggcctgctgg atcggcaata tcgccatggt ggtcattggc
     3841 gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt aaccatcacc
     3901 tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa aatgatcacc
     3961 cgcgtgcagg ccgttgccac tgtgctggcg ctgatcccca tcgtcgggat tgccgtattt
     4021 ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag cggcctgggc
     4081 accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat cggtgtggaa
     4141 agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc tatcgccacc
     4201 attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc gattatgggg
     4261 atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc cgcacggatg
     4321 gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg ttgcttaggt
     4381 tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc cgatgacgga
     4441 ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc ggggctgatt
     4501 atcgtcggta ttttgatgac catcttccag ctcagcagca tttcaccaaa cgcgaccaaa
     4561 gagttcggtc tggtttcttc cgtctcggtc atctttacac tggtgccata tctttacacc
     4621 tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc ggcatatctg
     4681 gctgttacta ccattgcctt cctctactgc atttgggccg tggtggggtc cggggcgaaa
     4741 gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc gctgaattac
     4801 aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga ttaattcccc
     4861 ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa ttttgctttg
     4921 cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc tactaaaaag
     4981 accctctttg aatttactcg cctggctatt gttggccgct ttttatatct ctatctgcct
     5041 gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc tgcataacgt
     5101 actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg tcctgacact
     5161 aagctctttc ttatggctta atcgaacact ggcctgcctg tttattctgg ttggcgcggc
     5221 tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga ttgccaatat
     5281 tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt tattaacgct
     5341 gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca aacctgccac
     5401 ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg tactgctgat
     5461 tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca ataacaaaga
     5521 gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat ggtactccca
     5581 tcagcgactg gcaaatctgc cgctggtgcg aattggtgaa gacgcgcacc gcaacccgtt
     5641 aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa cctcgcgggc
     5701 ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg cgaaagataa
     5761 cgtggtctat ttccctaata ccgcatcttg cggtacggca acggcagttt cagtaccgtg
     5821 catgttctcg gatatgccgc gtgagcacta caaagaagag ctggcacagc accaggaagg
     5881 cgtgctggat atcattcagc gagcgggcat caacgtgctg tggaatgaca acgatggcgg
     5941 ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga atctgcctga
     6001 tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg aagagtacat
     6061 caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc acggtccgac
     6121 ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg acaccaatga
     6181 gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc tggtttacgt
     6241 cgactatatt gttgataaag cgattaatct gcttaaagaa catcaggata aatttaccac
     6301 cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca tctatctgca
     6361 cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccaatgc tgctgtggct
     6421 gtcggaggat tatcaaaagc ggtatcaggt tgaccagaac tgcctgcaaa aacaggcgca
     6481 aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa ctggcgttga
     6541 gacgaagtat taccaggccg cggatgatat tctgcaaact tgcaggagag tgagtaaatg
     6601 aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct ggcggcgcaa
     6661 accgaaggct acgcgtgcga tggcgtgaca accgcgcgga tggcggaaca aagccttgag
     6721 gacggtcatt acagcctggt ggtactggat ttagggttac ccgacgaaga tggactgcat
     6781 tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct caccgctcgc
     6841 gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta tctggtgaag
     6901 ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg ccataataat
     6961 cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg ccgtcaggta
     7021 tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct gtcacggtta
     7081 atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat ctataactgg
     7141 gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg cgacaaagtg
     7201 ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc gaatgaggaa
     7261 aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca acggctgata
     7321 ttgaccatcg gggccatttt gttggtgttt gagctgatca gcgtcttctg gctatggcat
     7381 gaaagtaccg agcagattca actgtttgag caggcgctac gcgacaatcg caacaacgat
     7441 cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc cggcgtcttt
     7501 atggtcagcc tgacgctatt tatctgctat caggcggtac gccgcatcac ccgcccgctg
     7561 gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc cattgccatt
     7621 cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct ggtcagtcgc
     7681 ctgaccagca cgctggataa cgaaaggttg tttaccgctg acgtcgcgca cgaactgcga
     7741 acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca tcacattgat
     7801 gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca gctgctgcaa
     7861 ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa actgctggaa
     7921 gatgtcattc tcccctcgta tgacgaactc agtaccatgc tcgaccagcg acagcaaacc
     7981 ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac cctgctgcgg
     8041 atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccgcaagg cagcaacatt
     8101 atgattaagc tgcaagaaga tggcggagcg gtcatggcag ttgaagatga aggaccaggt
     8161 attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga cagccgttat
     8221 ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca tcacgggcag
     8281 tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg gctgaagaaa
     8341 gatcagaacg tggcaaacca gatataaaga aagctgctga ccaccagcac gctgaacacg
     8401 gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa cctggaggag
     8461 agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat aagacagcgt
     8521 cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg caccaggcgg
     8581 gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat tatcgatttt ctgctcgata
     8641 ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc cgccggtgtc
     8701 gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat taaaccaatc
     8761 accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct ttcgaccagc
     8821 caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa tgccgccgcc
     8881 agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc cataacgccc
     8941 ataaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag gccgatgacg
     9001 ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagtgcaac actaccaagt
     9061 agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg ctggacaaac
     9121 agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga gtaatgcagg
     9181 ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac gttggtggca
     9241 attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat ctctttaaac
     9301 gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt atcaacatgc
     9361 tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat aatccctaac
     9421 ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc ttcgccgaca
     9481 atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat agaaccgaag
     9541 tccagccagc tgcccataaa gccacgttta cggtcggggg agtattccgc aacaaatatc
     9601 gacgccccgg tatattcacc accgaccgag aaaccttgtg ccatcttaca gatcaacagc
     9661 agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat acagaacgta
     9721 ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata tttatcgccc
     9781 aacataccaa agaagagtcc gccaagcggt cgaatcagaa agggaacgga gaaagtggca
     9841 agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt acctaatgcg
     9901 taagcaacaa aaccataaac accaaaatca aaccattcca ttgcattacc cagtgatgct
     9961 gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg aagggtaatc
    10021 ggttttactt ttttcctttt cagcatagct ttcctcgcag agttgaccca tttccgcaaa
    10081 cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg atgcaatctc
    10141 tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca atcaaaccag
    10201 ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta aatttgtgat
    10261 caacttcaca catggatttc gtttaggact cattgatgta actgattatt tacgccaaat
    10321 ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa cagagtcgtg
    10381 gcatccccag cgcgacagtt tgccagcgtt gggtcactga ccagcaaacg ggttgtttat
    10441 ctgccgtggt ccactttccc agccaactta aaaaatcagc atgatccccg cccgtcggaa
    10501 cggtcgaaga ggcaaaccac tgatggtaat agtgacgcgt ggtcggtgcc gcattgatac
    10561 tctctgccag atgttgtgtc gctttctcgc gcaaatcacc cagcaacaac accaccgctt
    10621 gcgggcttcc cagacggcag gcatcgccaa atgcacccca gcgcaactcc tccagaacga
    10681 taaaacagcg cccaggcaac gaccaggcgt tatacgcccc agcacgccag cgggtaaaaa
    10741 tttgctcgct ctgatcacct gcctgcacca gtaggcctcg ttgcgttaac gccttttcct
    10801 tccagcgaca acgggctgaa aaccactcat acaaggcggc aatttgctgg gggagctccg
    10861 ggaaagcaga gtgctggcgg tgatccattt tttgcagcag cgccagatgc tgcgcgatct
    10921 gcgtcagcgc cagatgaccg ggatccagca tcgacgccag tttttccacc attgtcagtc
    10981 gcagactaag cggcgcattt aacatgccgc tgttcgtcca tgtctgaagc tgcacacgcg
    11041 aaagaagttc ctgcatcagt cgttcacgaa actgctgctg atgggcttga ggatggcggg
    11101 catcatcgcc ctgcgccaga tccactaaaa agcgcggatg aaccgactcc agcacgcgac
    11161 cggggccgtc cagtaacgtc ttggtcaata tcgttctgcc gtgaaaagtg tttgaatatc
    11221 atcgcgtaac agctgagcgt cggtgtaaat ccagctgtga gtcatcacag tctgctgcaa
    11281 ttgctgctgc atcagcctga ccaccgattc attttgttga cgcagagcca gactttcgcg
    11341 taaacgcgtc tgtaattctg tcaaacatga agcgaactca gcgaaaaaag tattcatgcc
    11401 tgccgtaaca gattcatcga cctgctctgc cagaacttta gccatttgtt ggcaatagag
    11461 atcgacttct gcgcttaatg ctcgttgcaa cacactgtaa tcaaccgttt ctgtcgggga
    11521 tttctcattt ccccgtcccc agtcgggctg attcaaccag cgcgaaaaag tctcacgcac
    11581 aacgcctaaa cgcgtgctct gctcgtccgt tgcttcctgg cgcgaaatga cttcactgaa
    11641 cagctggcga gtgttgaagt ggggaactac gccgtgaaaa acaggaaaat gaaacccagg
    11701 acgaaaccct gactcgctca attccatttt gacttgttgc tcaatggggc gaataacatc
    11761 ggttaacacc cggcaaaggg tggattccag ctcggcaaaa cgcagcgtaa agtcgcgact
    11821 gatggtgttc tgcgccgtct gtaacagtgt ctcacagcgg gtacgaatct cgcttaacgg
    11881 ctccgaatca tcctgaaaca aggcggctaa ctgcgcattc accgcatctt gctgttgacg
    11941 cagaaagtgg ttggcggagg tcagggccag ctcgatttca tgtttaatct cgccgctcac
    12001 ctgcgcctga ttgagttgca atagctgcaa actttcttcg acctgatgga tattttgccg
    12061 caattgttca caagcgacgt ttaacccgtg cgcacgaaaa tccaggtatt cccgcgcctg
    12121 ctgcgcgtaa ttcaacagtt tatgcgcagc agatcgcaga gcatacaatg aggcgttagc
    12181 gtaagcggca tgaagcaacg cctgaattgg ctgggcgaac agcgaatctt cccacaactg
    12241 atcggcagca tgacgaatat gttcgaggtc cgccagatcg gcatgacgcc agcgcctgcc
    12301 gagcgcggca tgggcaaaat cttccaccca gcgttgttgc tctggcgctg gtaacttacc
    12361 gttgttggct aactcatagc gcgcccgatt cgccaggtag ccccacatcg acgacaccgg
    12421 gaatatctgc tgtggcgtaa tacagccttt catcagcgtc ccggaaatca gtgcccgcac
    12481 ctggtcggcg tcgtcactgt tacgatcctg ttgatcgaac ttattgacca gcacatacag
    12541 cggcaccgat tgccccaccg ccaaaatcgc ctcacggacc tcttcatcgg agatcgattt
    12601 cagttgcgta taatccagca ccgccagtac cgccgaggcg cgtgccagct gctggttaag
    12661 cattttttgc agatgcggtt gcccggcttc atttggcccg ggggtatcca gtaacgtcaa
    12721 ctgaccggga taactttcca gccccgccag atggacaaac tccacttcaa tcacgggaat
    12781 atgctcaatg gcggcgtaag cagaaaaagg aaaatcgacg tccagcgcct tcgccagtcg
    12841 cactaaatca ttcaaacttt tcagacaatg aaaaataggc tgggcaccca gataatattt
    12901 ttcgaaagcg acgccatttt cgatccgctg cataagcgca cgcatatctt tatctatttc
    12961 cagcacatcg gtcagatgct taatatcgca atcacgcagg cgctgttgta attgttgaat
    13021 taaacaatcg attggcgcga catgtgaaaa atgcagtacc ggttcctttt gcccgggcgt
    13081 atggcgaata agcgtcggca gcgcagtcat tgggcgatta cgattaggca gaacctccgt
    13141 accaacaatg gcattaatgg tggttgattt ccctgctttc atggtaccga caattgccag
    13201 caccatttcc agtcgggaaa ttttacgcaa ctcattattc agcatcgcgt gacgttcggc
    13261 gatattaggc tgactccagg gtaaagccag ttgtggcgcg tcgtctccgg gtacagagag
    13321 aggcattttt tccagtaact gcaactgttg gcgagaaagc tgtaacaggc gttcagcctc
    13381 ctgacttaac tcatacaggg tctgtgtgta catagaaaat tcttccttaa agcaaatttt
    13441 gttactttta tttagccaga ttgtttttga gttctgtttt cggcttttat aattactgca
    13501 agaaataatt ttatatttag tgtgttgttt tttatcagaa taaataacgt cttctgatgc
    13561 gtttaaaacg tcagaaagat aaaaatatca tgtaaataaa aaaaagaaca agtagagcat
    13621 taacattatc ttaaataata aatagaggca aaaagattat tttctttttg cgtttccttt
    13681 caaatgaaaa cgatcgtcgt ctaaaatcag cagtaccccc gacaaactca gggattttgt
    13741 gtataattgc ggcctttttc ggcaatctgc cgttttttgg cgcttttgcc ctgctgactt
    13801 ttgaggaaat ccacatgtca ttaccacact gcccaaaatg caactccgaa tacacttacg
    13861 aagataacgg catgtacatc tgcccggaat gtgcctacga atggaacgac gcagaacctg
    13921 cacaggaaag cgacgagctg atcgttaaag atgctaacgg caatctgctg gctgacggcg
    13981 acagcgttac catcattaaa gatctgaagg tgaaaggtag ctcttcgatg ctgaaaattg
    14041 gcaccaaagt gaaaaacatc cgcctggttg aaggcgacca taacatcgat tgcaaaatcg
    14101 acggttttgg tccgatgaaa ctgaaatctg agtttgtgaa aaagaactga ttgtattgtg
    14161 atcggtaagc cgaataaggc gctcgcgccg catccggcaa cgatgccgac tgcctgatgc
    14221 gacgcttgcg cgtcttatca ggcagcctct catctgtata aatttcgaac tacacttaac
    14281 tggcttctct taactgaggt caccatcatg ccgttaagtc cctacctctc ttttgccggt
    14341 aactgttccg acgcgattgc ctattatcaa cgtacgttgg gcgcggaact gctctataaa
    14401 atcagcttcg gcgaaatgcc gaaatcagcg caggacagcg ccgagaactg cccttccgga
    14461 atgcaatttc ccgataccgc catcgctcat gccaacgtgc gcattgccgg aagcgacatc
    14521 atgatgagcg atgctatacc gtcaggaaaa gccagctact ccggctttac gctggtgctc
    14581 gattcgcaac aggtcgaaga aggaaaacgc tggtttgata accttgccgc taacggaaaa
    14641 atcgaaatgg cctggcagga aactttctgg gcgcatggct ttggcaaagt caccgataaa
    14701 tttggcgtac cgtggatgat taatgtcgtc aaacaacaac caacgcaata acccgccggg
    14761 aggcccgccc tcccgcactg tcatcgaatt cccgttaact cttcatctgt tagtcacttt
    14821 taattaacca aatcgtcaca ataatccgcc acgatggagc cactttttta gggaggctgc
    14881 atcatgcaaa cgattatccg tgtcgagaag ctcgccaaaa ccttcaatca gcatcaagcg
    14941 ctgcatgcgg ttgatctgaa cattcatcac agtgaaatgg tggctctgct tgggccgtcg
    15001 ggttccggca aatccaccct tttacgtcat ttaagcggtt tgattaccgg cgataaatcc
    15061 gccggtagcc atatcgagct actgggccgc actgtccagc gcgaaggccg cctggcccgc
    15121 gatatccgca aaagccgcgc caacaccggc tacatctttc aacaattcaa cctggtgaac
    15181 cgcctgagcg tactggagaa cgtgctgatt ggcgcgctcg gcagcacgcc gttctggcgc
    15241 acctgtttta gctggttcac cggcgagcag aaacagcgcg cgttacaggc gctgacccgc
    15301 gttggcatgg tgcattttgc ccatcagcgc gtttccaccc tctccggcgg ccagcagcaa
    15361 cgtgtggcga ttgcccgtgc gctgatgcag caggcgaaag tgattctggc cgatgaaccc
    15421 atcgcctcgc tggacccgga atcagcgcgc atcgtgatgg acaccctgcg cgacatcaac
    15481 cagaacgacg gcatcaccgt ggtcgtcacg ctgcatcagg tggattacgc cctgcgctac
    15541 tgcgaacgca tcgtcgccct gcgtcagggg cacgtcttct acgacggcag cagccaactg
    15601 tttgataacg aacgttttga ccatctctac cgcagcatta accgcatcga agagaacgcg
    15661 aaagctgcct gatatcccca tcattgagga aaacgaatga acgctaagat aattgcctcg
    15721 ctggccttca ccagcatgtt cagcctcagc accctgttaa ccccggcaca cgccgaagag
    15781 caggaaaagg cgctgaattt cggcattatt tcaacggaat cacagcaaaa cctgaaaccg
    15841 caatggacgc cgtttttaca ggatatggag aagaaactgg gcgtgaaggt caacgccttc
    15901 tttgccccgg actacgcggg cattatccag gggatgcgct tcaataaagt ggatattgcc
    15961 tggtacggca acctgtcggc gatggaagcg gtggatcgcg ccaatggcca ggtcttcgcc
    16021 cagacggtcg cggcggatgg atcgtcgggt tactggagcg tgttgatcgt caacaaagac
    16081 agcccgatca acaacctgaa cgatctgctg gcgaagcgga aagatctcac cttcggcaat
    16141 ggcgatccta actccacctc tggcttcctc gtccccggtt actacgtctt cgctaaaaac
    16201 aatatctccg ccagcgactt caagcgcacc gtcaacgccg ggcatgaaac caacgcgctg
    16261 gccgtcgcca acaagcaggt ggatgttgcc accaacaaca ccgaaaacct cgacaagctg
    16321 aaaacctccg cgccggagaa gcttaaagag ctgaaggtga tctggaaatc gccgctgatc
    16381 ctaggcgatc cgatcgtctg gcgcaagaat ctgtccgaaa ctaccaaaga caagatctac
    16441 gacttcttta tgaactacgg aaaaacgccg gaagagaaag cggtgctgga acgcctaggc
    16501 tgggcgccgt tccgcgcttc cagcgacctg caactggtgc cgattcgcca gctcgcactg
    16561 tttaaagaga tacaaagcgt gaaaggcaat aaagggctga atgagcagga caagctggcg
    16621 aaaaccaccg agattcaggc gcagctggat gacctggacc gcctgaacaa cgcgttaagc
    16681 gcgatgagtt cggtgagtaa agcggtgcag taaatcgtag gtcggataag acgccccggc
    16741 gtcgcatccg acaatgtgca ggcgttgatg ccggatgcgg tgcaagcacc ttatccggcc
    16801 tacagaccgg agccaaacat gcaaaccatc accatcgccc cacccaagcg tagctggttc
    16861 tcgcttctga gctgggccgt tgttctcgcc gtgctggtcg tctcgtggca gggcgcggaa
    16921 atggccccgc ttacgctgat taaagacggc ggcaacatgg caaccttcgc tgccgacttc
    16981 ttcccacccg atttcagcca gtggcaggat tacctcaccg aaatggccgt cacgctgcaa
    17041 atcgccgtct ggggcaccgc gctggcagtg gttctctcca tccccttcgg tctgatgagc
    17101 gccgaaaact tggtgccgtg gtgggtttac cagcccgttc gccgcctgat ggacgcctgc
    17161 cgcgccatta acgaaatggt cttcgccatg ctgttcgtgg tcgccgtcgg cctcggcccg
    17221 ttcgctggcg tgctggcgct gtttatccac accaccggcg tgctctccaa gctgctttcc
    17281 gaagcggagg aagcgatcga acccggcccg gtggaaggca ttcgcgccac cggcgccaac
    17341 aaactcgaag agatccttta cggcgtgctg ccgcaggtga tgccgctgct gatctcctac
    17401 tccctctatc gcttcgaatc caacgtccgc tcggcgaccg tcgtcggcat ggtcggcgcg
    17461 ggcgggatcg gcgtcaccct gtgggaagcg attcgcggtt tccagttcca gcaaacctgc
    17521 gccctgatgg tgcttatcat cgtcacggtc agcctgctgg atttcctctc tcaacggttg
    17581 cgtaagcact ttatctgata agcgaggcat tgatatctat gcacttgtct acacatccga
    17641 ccagctaccc aacacgctat caggagatag ccgcaaaact tgagcaggag cttcgtcaac
    17701 actaccgctg cggcgactat cttcccgccg agcagcaact ggcggcgcgc tttgaggtga
    17761 accgccacac cctgcgccgc gccatcgacc aactggtgga aaaaggctgg gtacagcgcc
    17821 gtcagggcgt cggcgtgctg gtgctgatgc gcccgttcga ttacccgctc aacgcccagg
    17881 cgcgttttag ccagaacctg ctggaccagg gcagccatcc caccagcgaa aaactgctgt
    17941 cggtattgcg ccccgcgtcc ggtcacgttg ctgacgcgct ggggattacc gagggcgaga
    18001 acgttatcca tttgcgcacc ctgcgccggg tcaacggcgt cgcgctctgt ttaatcgacc
    18061 actactttgc ggacctcacc ctttggccga cgctgcaacg cttcgacagc ggttcgctgc
    18121 acgattttct gcgcgagcaa accggaattg cgctgcgccg cagccagacg cggatcagcg
    18181 cccgccgcgc ccaggccaaa gagtgccagc gccttgaaat cccgaatatg tcgccgctgc
    18241 tgtgcgtgcg cacccttaac caccgtgacg gtgaaagcag cccggcggag tactccgtca
    18301 gcctgacgcg cgccgacatg attgaattca ctatggagca ctgaatgcac gcagataccg
    18361 cgacccgcca gcactggatg tccgtactgg cgcacagcca accggctgaa ctggcagccc
    18421 gcctgaacgc gctgaacatc accgccgact atgaagtgat ccgcaccgcc gaaaccggcc
    18481 tggtacagat tcaggcgcga atgggcggca ccggcgaacg cttttttgcc ggcgacgcca
    18541 cgctgacccg cgccgccgta cgcctgactg acggcaccct cggctacagc tgggtgctgg
    18601 ggcgtgataa acagcacacc gaacgctgcg cgctgattga tgcgctgatg cagcaatctc
    18661 gccactttca aaacttatca gaaaccctta ttgccccgct ggacgctgac cgtatggcac
    18721 gcattgccgc acgccaggcc gaagtgaacg ccagccgggt cgacttcttt acgatgattc
    18781 gcggagacaa cgcatgaccc tggaaaccgc ttttatgctt cccgtgcagg atgcccagca
    18841 cagttttcgt cgcctgttaa aggccataag cgagccgggc gtgattgtcg ccctgcatca
    18901 gctcaagcgc ggctggcagc cgctgaatat cgccaccacc agcgtgctgc tgacgctggc
    18961 tgataacgac acgccggtgt ggcttgccgc gccattgagt aacgatatcg tcaatcagag
    19021 cctgcgtttt cataccaacg cgccgctggt cagccagccg gaacaggcga ccttcgcggt
    19081 gacggatgac ggaatttcca gcaaacagct caacgccctt tccaccggca ccgccgttgc
    19141 gccggaagcg ggcgcgacgc tgattttaca ggtcgccagc ctgagcggcg ggcgcatgtt
    19201 gcgtctcacc ggcgcgggta ttgccgaaga acgaatgatc gctccgcagc tgccggagtg
    19261 cattctgcac gaactcaccg agcgcccgca cccgttcccg ctcggcatcg acctgatcct
    19321 gacctgcggc gaacgcctgc tggctattcc gcgaaccact catgtggagg tgtgctgatg
    19381 tacgttgccg tgaaaggggg cgaaaaggcg atcgacgccg cccacgccct gcaagagagc
    19441 cgacgccggg gcgataccga tttgcccgaa ctgagcgtcg cccaaattga acagcagctt
    19501 aatctcgcgg tggatcgcgt gatgaccgaa ggcggcgttg ccgaccgcga actggcggcg
    19561 ctggcgctga aacaggccag cggcgataac gttgaagcaa ttttcctgct gcgcgcctac
    19621 cgcaccacgc tggcgaaact ggcggtaagc gagccgctcg acaccaccga gatgcgtctc
    19681 gaacggcgta tttcggcggt ttataaagac attcccggcg gccagctgct tggcccaacc
    19741 tacgactaca cccatcgcct gctcgatttc accctgctgg caaacggtga agcgccgacg
    19801 ctgaccaccg ccgacagcga acaacagccg tcgccgcacg ttttcagcct gctggcgcgt
    19861 caggggctgg cgaagtttga agaagatagc ggcgcacagc cggatgacat cacccgcacg
    19921 ccgccggttt acccctgctc acgctcctcc cgcctgcaac aattaatgcg cggggacgaa
    19981 ggctatttgc tggcgctggc ctactccacc cagcgtggtt acggacgcaa tcacccgttc
    20041 gcgggcgaga tccgcagtgg ttacatcgac gtgtcgattg tgccggaaga gctgggattt
    20101 gcggtaaacg tcggcgaact gctgatgacc gagtgtgaaa tggtcaacgg ttttatcgac
    20161 ccgccggatg agccgccgca cttcacgcgc ggctacgggc tggtattcgg catgagcgag
    20221 cgcaaagcga tggcaatggc gctggttgac cgtgcgttgc aagccccgga atacggcgag
    20281 cacgcgacag gcccggcgca ggatgaagag ttcgtgctgg cgcatgccga caacgtcgaa
    20341 gccgcaggct ttgtctcgca cctcaaactc ccccactacg tcgatttcca agccgaactg
    20401 gagctactca aacgtctgca acaggagcag aaccatggct aatctgagcg gctacaactt
    20461 tgcctacctc gacgagcaga ccaaacgcat gatccgccgc gccatcttaa aagcggtggc
    20521 tatccccggt tatcaggtgc cgtttggcgg gcgcgagatg ccgatgccct acggctgggg
    20581 aaccggcggc attcagctca ccgccagcgt gattggcgaa agcgacgtgt tgaaggtgat
    20641 tgaccagggc gcagacgaca ccactaacgc cgtgtcgatt cgcaacttct tcaagcgcgt
    20701 gaccggggtg aacaccactg aacgtacgga cgatgcgacg cttatccaga cgcgccatcg
    20761 catccccgaa acgccgctga ccgaagatca gatagttatt ttccaggtgc cgatccccga
    20821 accgctgcgc tttatcgagc cgcgcgaaac ggaaacccgc actatgcacg cgctggagga
    20881 gtacggcgtg atgcaggtga aactgtatga agatatcgcc cgcttcggtc atatcgccac
    20941 cacctatgct tatccggtga aggtgaacgg gcgctacgtg atggacccgt cgccgatccc
    21001 gaaattcgat aacccgaaaa tggacatgat gcccgccctg cagctgttcg gcgcagggcg
    21061 cgagaagcgc atctatgcgg tgccgccgtt tacccgcgtg gaaagtctcg atttcgacga
    21121 tcacccgttc accgttcagc agtgggatga gccatgcgcc atctgcggat cgacccacag
    21181 ctatctcgac gaagtggtgc tggatgacgc cggaaaccgc atgtttgtct gctccgatac
    21241 cgattattgc cgccaacaga gcgaggcaaa aaaccaatga atcaaccgtt actttccgtc
    21301 aataacctga cccaccttta cgcgccgggc aaaggcttta gcgatgtctc ttttgattta
    21361 tggccggggg aagtgctggg catcgtcggg gaatccggct ccgggaagac cacgctgctg
    21421 aaatcaatct ccgcgcgcct gacgccgcag cagggggaga ttcgctacga gaatcgttcg
    21481 ttgtatgcga tgagcgaggc cgaccgccgt cgcctgctgc gcaccgaatg gggcgtggtg
    21541 catcagcatc cactcgacgg cctgcgtcgc caggtctcgg caggcggcaa tatcggcgag
    21601 cggctgatgg cgaccggggc ccgtcattac ggcgacatcc gcgccaccgc gcagaagtgg
    21661 ctggaagagg tggagattcc cgccaaccgg atcgacgacc tgccgaccac cttttccggc
    21721 ggcatgcagc aacgtttgca gattgcccgc aacctggtga cgcatccgaa gctggtgttt
    21781 atggacgagc caaccggtgg gctggatgtg tcggtgcagg cacgcctgct cgatctactg
    21841 cgcggcctgg tggtggagct gaacctggcg gtggtgattg tcacccatga tttaggcgtc
    21901 gcccgcctgc tagcggaccg tttgctggtg atgaagcagg ggcaagtagt ggagagtggg
    21961 ttaaccgacc gcgtgctcga cgacccgcac catccgtata cccagctgct ggtgtcatcg
    22021 gttttgcaga attgagccgg tgccggatgc ggcgtaaacg ccttatctgg cctacaaatg
    22081 cgctccccgt aggtcggata agacgcatca gcgtcgcatc cgacacccga accacgaggc
    22141 gaaaaatgat taacgtacaa aacgtcagta aaaccttcat cctgcaccag caaaacggcg
    22201 tgcgcctgcc cgtcctcaac cgcgcctcgc tcaccgtcaa cgcgggcgaa tgcgtggtgc
    22261 tccacggcca ctccggcagc ggcaaatcaa ccctgctgcg ctcgctgtac gccaactatc
    22321 tgcccgatga agggcaaatc cagatcaaac acggtgacga gtgggtagac ctggtcaccg
    22381 cgccagcgcg caaagtggtg gagatccgca aaaccaccgt cggctgggtg agccagtttc
    22441 tgcgcgtcat cccgcgtatt tcagcactgg aagtggtgat gcagccgctg ctcgataccg
    22501 gcgtaccgcg tgaagcctgc gccgctaaag ccgcgcgtct gctcacccgc ctgaacgtgc
    22561 cggaacgcct gtggcacctg gcgccatcaa ccttctccgg cggcgaacag cagcgcgtca
    22621 acatcgcccg cggctttatc gtcgactacc cgattctgct gctcgacgaa cctaccgcct
    22681 cgctggacgc caaaaacagc gccgcggtgg tggaactgat ccgcgaagcc aaagctcgtg
    22741 gcgcagccat tgtcggcatc ttccatgacg aagccgtacg taatgacgtt gccgaccgcc
    22801 tgcacccaat ggaagcctct tcatgattat caataacgtc aaattggtgc tggaaaacga
    22861 agtggtacac ggttcactgg aggtgcagga cggcgaaatc cgcgcctttg ccgaaagcca
    22921 gagccgcctg ccggaggcga tggacggcga aggcggctgg ctgctgccgg ggctgattga
    22981 gctgcatacc gataatctgg ataaattctt caccccgcgc ccgaaagtcg actggcccgc
    23041 ccattcggcg atgagcagcc acgacgcgct gatggtggca agcggcatca ccaccgtact
    23101 ggacgccgtg gcgattggtg atgtgcgcga cggcggcgat cggttggaga atctggagaa
    23161 gatgatcaac gccatcgaag agacgcagaa acgcggcgtc aaccgcgccg agcaccgtct
    23221 gctcctgcgc tgcgaactgc cgcatcacac cacgctgccg ctgtttgaaa aactggtgca
    23281 gcgcgagccg gtgacgctgg tgtcgctgat ggaccactcg ccgggccagc gccagttcgc
    23341 caaccgcgag aagtatcgcg aatattatca gggcaaatac tcgctcaccg atgcgcagat
    23401 gcagcagtac gaagaagagc aactggcgct tgccgcacgc tggtcgcagc cgaatcgcga
    23461 atccatcgcc gccctgtgcc gcgcgcgaca aattgcgctc gctagccatg atgacgccac
    23521 ccacgcccac gttgccgaat cccacctgct tggcagcgtg atcgccgaat ttcccaccac
    23581 gtttgaagcg gcggaagcct cgcgcaagca tggcatgaac gtactgatgg gcgcgccgaa
    23641 tatcgtgcgc agcggttcgc actccggcaa cgtggcggcc agtgaactgg cgcagcttgg
    23701 cctgctggat atcctctctt ccgactacta ccccaccagc ctgctggatg cggcatttcg
    23761 cgtcgccgat gaccagagca accgctttac gctgccgcag gcggtgaagt tagtgaccca
    23821 aaatccggcg caggcgctta atcttcagga tcgcggggtg attggcgagg gcaaacgcgc
    23881 ggacctggtg ctggcacatc gcaaggacaa tcatattcat atcgaccacg tctggcgtca
    23941 gggtaaaagg gtgttctgat gatgggaaaa ctgatttggt taatggggcc gtccggctcc
    24001 gggaaagaca gcctgctggc ggaaatccgc ctgcgggaac aaactcagtt actggtggcg
    24061 catcgctaca ttacgcgcga cgccagcgcc ggaagtgaaa accatatcgc cctgagcgag
    24121 caggagtttt ttacccgcgc ggggcaaaat ctgttggcct taagctggca cgctaacagt
    24181 ctgtattatg gcgtcggcgt tgagattgat ctctggctgc acgccggatt cgacgtgctg
    24241 gtcaacggct cacgcgccca tctgccgcag gcgctggcgc gctatcaatc ggcgctgctg
    24301 cccatttgct tactggtttc gccggagatc ctgcgccagc gcctcgaaaa ccgtggtcgt
    24361 gaaaatgcca gtgaaattaa cgcccgacta gcacgcgctg cccgctatac tcctcaggat
    24421 tgtcttacgc tcaataatga cggcagcctg cgccagtcgg tcgacaagct gctgacgctg
    24481 attcatcaga aggagaaaca ccatgcctgc ttgtgagctt cgcccggcta cgcagtacga
    24541 caccgacgca gtttacgcgc tgatttgtga gctaaaacag gcggagtttg accaccatgc
    24601 gtttcgcgtg ggctttaacg ccaacctgcg cgacccaaac atgcgctacc atctggcgct
    24661 gctggatggc gaagttgtca gcatgatcgg cctgcatttg cagtttcatc tgcatcatgt
    24721 caactggatc ggcgaaattc aggaactggt ggtgatgccg caggcgcgcg gtctgaacgt
    24781 cggcagtaaa ttactggcat gggcagaaga agaagcccgc caggccgggg ccgaaatgac
    24841 cgcgctttcg accaacgtga aacgccacga cgcgcaccgt ttctatctgc gagaaggcta
    24901 cgagcagagt cacttccgct tcaccaaggt gctgtaacat gagcctgacc ctcacgctca
    24961 ccggcactgg cggcgcacag ggcgttccgg catggggctg cgagtgcgcg gcctgcgacc
    25021 gagcgcagcg ctcaccgcag tatcgccgcc agccatgcag cggcgtagtg aagtttaacg
    25081 acgcaatcac cctgatcgac gccgggctgc acgatctcgc cgatcgctgg tcgcccggat
    25141 cgttccagca gtttttgctg acgcattatc atatggatca cgcccagggg ctgtttccgc
    25201 tgcgctgggg cgtgggcgat ccgatcccga tctacggccc gccggatgac cagggttgcg
    25261 acgatctgtt taaacatccg ggcctgcttg atttcagcca cacagtggaa ccgtttgtgg
    25321 tgtttgattt gcaggggttg caggtcacgc cgctgccgct taaccactca aaactgacct
    25381 tcggttatct gctggaaacg gcacatagcc gggtggcgtg gctatctgac accgctggcc
    25441 tgccggaaaa aacgctgaaa tttttacgca ataatcagcc acaggtaatg gtgattgatt
    25501 gcagccaccc gccgcgcgcg gatgcgccgc gtaatcactg tgatttaaat accgtgcttg
    25561 cgctgaatca ggttatccgc tcgccacggg tgattctgac ccatatcagc caccagtttg
    25621 atgcgtggct gatagaaaac gcactgccgt cggggtttga ggcggggttt gatgggatgg
    25681 agattggggt ggcgtgatga gagggaatgt gcgcgctggt cccctcaccc taaccctctc
    25741 cccagagggg cgaggggagt gattgtgctc gatattgaat attgcgctgg ttttctccct
    25801 ctccccattg gggtgagggg cgatgcctgc tccataccca acctcatcgc ccatactcat
    25861 cttccattct ccgctcttca tcttctagct gccgacgctc ctgatcaagc tggcgctggc
    25921 gatcgtccag ctgcctgcgg cgatcttcaa actggcagcg gcggtcgtca tattgtctgc
    25981 gccgatcgtc gctcacttca cgctgccagc cgtcgtcgcg cgaatcttca tagtctcgcc
    26041 cacggtcaga gttataagcg tcattaatcg cctgctgaat attaccaata gtatcgtcga
    26101 taatatcggc ctgggccgga acggtggaca gcgtgagcag ggtgaaaaga aaaagcgtgg
    26161 agtaaggttt cataagccag tctctgcgat ggactggctt aacgttagtt gagtgtgaat
    26221 gaccggagta gcggagtaat ctcaaattcg gtaaggcgca gactcttcca ttagctggtt
    26281 agctcgctgt ttattttccg ccaccaatac aatgccatga catttttgat ccggtgaaat
    26341 actccagata cgctcttccg gaatgcagca gccatcgtag atcactttcg tgccatcgta
    26401 gggccaaaat gccccttgtt cagtgtctaa tacaaacacg gaaatctgat cggactcgcg
    26461 cgcgatcttc tgccagagca tcttcccgcc atcatattga tcaaaatcgc tgattagcgt
    26521 gataccgcat tgcaggatta gcgcttcata cagccgagtt gccagaccct tatcgcgctc
    26581 acctggagag acacaaatac cttccacctg taccagatcc gagtcttccc accagcgttc
    26641 aacggcaatg atatcgctgc cgaactcttt tttacgctgc agatcgagag cggcaagtac
    26701 ccgatgacgc ggcacgcctg caaaaacttc aatgtccgct tcaatgatgg ccgctcttgg
    26761 cgtctggttg tcataaatta cctgtaacag acgtccagaa agctcgatct cttccaccac
    26821 gcgaaaagaa cgagcaaccg tcatagggct ataggtggtt ggcatcacga tcatatgtaa
    26881 atcacttcaa ttcagaacag aattgctcac aatagcagca cgtttgcttc gagcctcttg
    26941 ataaattctg aaaatcatcc ctcaatttgc cccagcacct gcgccagctg cgcgcgggta
    27001 aagggtttgc gcagcagcgc cacgtccggc agcgcggggt tatggctggg gcgtaaatcc
    27061 tgaccgctga tgagtagcag cgtcaggtgc gggtaaagtt tgcgggccgc attgaccact
    27121 tctgcgccgc tcatgccgcc agcaggtcca gcgcctgttc gccgctgctc gcttccagtg
    27181 tcaaatagcc cagcaggtgc aactgctcgc agatggtctg gcgcaccgcg gcttcatctt
    27241 ccagcactaa caccagttta tcgccattaa cagctgtctg ttctaccgcc gctggcgata
    27301 ggtttttcgc cgccgtcagc gcgcggggaa gctgtaatct gaccgttgtc ccctgccccg
    27361 gcgcgctttc aatttccacg cgcccgccgg actggcgcac gaaaccatag accatcgaca
    27421 agcccagccc gctgccgctc cccgtctgtt tggtggtaaa gaacggctcg aacacctggc
    27481 ttttcaccgc ctgcgacata ccgctgccat gatcgatgac ctccagtgcc accatgtcct
    27541 gacgctgccc actgctgcgg gtaacacgct gattccaggt acgaattttg ataattcccg
    27601 tctgcccttc catcgcgtcg cgggcgttca tcaccaggtt gatgatggcg ttttccagtt
    27661 ggctgacgtc aatccacgcg gaccacgccg gagactgcgc ttcaatctca atcgtcagcg
    27721 tcgccggtaa ggagtggcgc attaattcgc ttaactcttc cagcagtgtt tttagttcaa
    27781 cggcttgcgg atgtaaggac tgcttgcggg aaaatgccaa caatcgctgg gtcagtagcg
    27841 cgccacgttc ggcggcttgc tgcgcccgct gtattcgcgg cgcgtcgggt gagttcgtgt
    27901 tcaccagttc caggctacca ataattaccg ccagcagatt attaaaatca tgggccagcc
    27961 cgcccgtcag ttgcctgacc gcttttaact tctggctgtg caatagcgcc tcttccagcc
    28021 cctgccgctc ggtttgggca atctccatct gcgtggtttt ctctttcaac agccgtgacg
    28081 ttcgttcaag tgatgcggta ttgcgggcaa aaacgttaaa ggcgcgcgcc agttcaccca
    28141 actcatcgcg ccgctgtaac gccggaaaag tgacgtccgc ttcgccgtgc gccaggcggc
    28201 tcatcgcgcg ggaaatcgcc gtcaggttag agccgagatt gcgatagata taccaacctg
    28261 caaacgcggt gatcaccacc gctaacaggg cgaaaatcat gatcagcctc tcgccggagc
    28321 gcaaatcctg gtaactttgc tccacccgct gaccagagag cgtcgccacc tgttcggcgt
    28381 aacggttgat gtcgccatta aggatcgcca ccagcgcttt gatgtgaaac atataccagc
    28441 tgattgccag atcgctttgc tccagagccg ccgaaagtgg cgcgagttta cgcagttcac
    28501 gattaaaatc agcgagtagc gtattaacca atggcgtatc gctgtgagtt ggcaaggcag
    28561 acatcactcc gaccagttga tgaataatcg cctgcggcgc gggggtggca atggcggcaa
    28621 caatcagtcg gttcatctct ttaagcaaaa tattatcttg cgcgtgagtg actttttgca
    28681 gatgccgcag ataacttaag ttttgataca gcgaacttaa cagcgcgtta cgctccagat
    28741 gccgcctttg cccgcgttcc agcattccgc cgacgctggt ttgcagttcg ttgctacgcg
    28801 tgatgatgcg cgtcaccagc ccggcttcct gctgcgctaa aggcgcgttc gccagttgtg
    28861 caagcgagct ttttaatgcc cgctgtgtct cctgcaaccg ctcggcttcg cctttatatt
    28921 ccagtgctcc caccacctgc gacaatcgca ctgccgccgt tgccacgttt gccgtgtcgc
    28981 gcgccagatt caggctgccg gtcatatcat ctacagtttg ctgcttcacc agctcttgca
    29041 tctggctggc atgacgaaag cccattaccg ccacgccgct gaccatcaac gtcaccgcca
    29101 ccaccagcaa attcaaaagc agcaatcgcc cgcgcgcgct ggcaaaaaag tgacgtccgg
    29161 ggtgcgccat atcacgctcc gtgaagaaaa atcgcaatat gacaaatatg aacggcttct
    29221 gacattttgc atttactttc ctgtgatgga gtgctgatat cgacaacctc gcaggagtcc
    29281 accatgaaca gcacgccggt tcttgagatg cgcaatattg ccaaagcctt cggcaaattt
    29341 tatgcgctca aaggggtgga tttgacggtc taccctggcg agatccatgc cctgatgggg
    29401 gaaaacggcg cgggaaaaag cacgctgatg aaggtgctgg cgggggcgta taccgccacc
    29461 agcggcgaga tcctgataga cggcaagccc tttcacattc gcacgccaaa agatgcctta
    29521 agcgccggta ttacgctgat ttatcaggag atgcagctgg cacccaattt gacggtggca
    29581 gaaaatattt tcctcggcag cgagctttcc cacggcgggc tggtgcagcg aaaagagatg
    29641 ctggtgcagg cgcaaagagt gatcgaccgc ctcggcgcgc agtttaacgc cagcgataag
    29701 gtcatgacgc tgaccattgc cgaacaacag caggtcgaaa tcgcccgcgc gctacatcgc
    29761 aacagccgca ttctggtgat ggacgaaccc accgctgccc tctcctcccg tgaaactcac
    29821 cgtctttttg aactgattat gcggctgcgc gatgaaggga tggcgattat ctacatcagc
    29881 caccgtatgg cggaagtgta tgagctttcc gatcgcgtca gcgtgctgcg cgacgggcaa
    29941 tacgttggca gcctgacccg cgataacctc aatgccgggg agctggtgcg gatgatggtc
    30001 ggcaggccac tgagcgatct gttcaataaa gagcgcgata tcccgctcgg taaagcccgc
    30061 ctgaatgttc accacctgac cgacggcggc aaagtccagc cgagctgcct gcaggtgctt
    30121 tccggcgaaa ttgttggcct cgccgggctg gtgggagccg gacgttccga actggcgcag
    30181 ttaattttcg gcgtgcggaa agcgacaggc ggaatgattg aagtcgatgg cgaaccggtg
    30241 gtgatccact ccccgcgcga agccatcgat cttggcattg gttttctcac cgaaaaccgc
    30301 aaagaacaag gcttattcct tgaaatggcg gcagtcgaaa acatcactat ggcaaccctg
    30361 gagcgcgatg ccagctgggg aatgctcaat cgcaaaaaag cgcaaaccat ttccgatgac
    30421 gccattaagt tgctcaacat tcgcgtgcct catgcccagg tacgcgcggg cgggctttcc
    30481 ggcggcaatc agcaaaaact gttgatctcc cgctgggtgg cgattggccc gcgcatttta
    30541 ctgctcgatg aacccacccg cggcgtggac gttggcgcaa aaagtgagat ctaccggatc
    30601 atgaacgaga tggcgcgcaa gggcgtggcg atcctgatga tctccagcga actgccggag
    30661 atagtgggaa tgagcgatcg cgtccatgtg atgcgcgaag gcagcattgc cggtgagcta
    30721 aatggcaaaa acatcaccca ggaaaacatt atgaccttag cgactggcgt caacgacgcc
    30781 cattcccagg cggtgacctc atgacgacct ctgcaaaaac ctctgccaaa aaaatgctga
    30841 tgagcgatct gatgcaaacg gtcggcattt tgcccatttt gatcctgatc gtagcggtat
    30901 ttggctttat cgcgccaaac ttttttaccg aaagcaacct gctaaatatt acccgccagg
    30961 cgtcgatcaa cattgtgctg gcggcgggga tgaccttcat cattttgacc ggtgggatcg
    31021 atctttctgt aggttcaatc ctcggcacca cggcggtggc ggcaatggtt gtctccctta
    31081 gcccggagtt cgccatgttg tctatcccgg cggcgctgat gctcggcctg ctgctggggc
    31141 tgttcaacgg cgcgctggtg gcctttgccg gattgccgcc gtttatcgtc acgcttggca
    31201 cctatacggc gctgcgcggg gcggcgtatc tgctggctga tggcaccacg gttatcaact
    31261 ccaacattaa tttcgagtgg ataggcaata actatctcgg gccggtcccg tggctggtgg
    31321 tgattgcgct ggcggtgatc gtgctgtgct ggtttattct gcgtcgcacc acgcttggcg
    31381 ttcatatcta cgccgtcggt ggcaatatgc aggcggcgcg tcttaccggc attaaggtct
    31441 ggctggtgct gctgtttgtt tatggcatga gcggcctgct ttcagggctt ggcgggatta
    31501 tgagcacctc gcggctgtac agcgccaacg gcaacttagg tatgggttac gaactggatg
    31561 ccattgcggc ggtgatcctc ggcggcacca gttttgtcgg cggcatcggc accatcaccg
    31621 gcacgctggt gggggcgcta attatcgcca cgctcaacaa tggcatgacc ttaatgggcg
    31681 tctcttactt ctggcagctg gtgatcaaag gggcggtgat catcattgcg gtgcttatcg
    31741 acaaataccg tacccgacat catcaaagtg cataacaaca ataccctaca catgaggaat
    31801 acagcatgag attgaagccc ttagtgaccg cgctctgtgc aggcgcgctg ctggccgcag
    31861 caccgttcgc ccaggcgaaa gatctgaaat cgataggcgt aacggtaggt gacctggcga
    31921 acccgttctt cgtacagatc accaaaggcg cggagctgga agcacgcaaa ctggcgggcg
    31981 ataacgtcaa agtgacgctg gtttccagcg gctacgatct gggtcagcag gtctcgcaga
    32041 tagacaactt tatcgccgct aacgtcgata tgatcatcct taacgctgcc gattcaaaag
    32101 ggatcggccc ggcggtgaaa cgggcaaaag acgccgggat cgtggtggtg gcggttgacg
    32161 tggcggcaga aggtgccgat gccaccatca cttccgataa cacccaagca ggcgaaatgg
    32221 cctgtaaata catcaccgat cgcctgaaag gcaaaggcaa cgtggtgatc atcaacggac
    32281 cgccggtgtc cgccgtgcaa aaccgcgtcg aaggctgcca gaccgaattt aaacgccatc
    32341 ctgacatcaa ggtgctctct gacaacaaga acgccaaagg cagtcgtgag ggcgggctgg
    32401 aggtgatgac cgggctgctg gcggcaaatc cgaagatcga cggcgtattt gcgattaacg
    32461 atccgacagc gatcggtgcc gatctggcag caaaacaggc gcaacgtaac gagttcttta
    32521 ttgtcggcgt ggatggaagc ccggacgccg aagaggcgct caagcgtgag aactcgttgt
    32581 ttgtcgctac tccggcgcaa gatccacagg tgatggcagc gaaagcggtt gagatcagtt
    32641 atgacatttt gcagggcaaa cctgcgccga aagagccggt gctgatcccg gtgacgatga
    32701 tcgataaaaa caacgtcggc aattacaaag gctggacggt gaaataagtc cacacgccgg
    32761 gcggcgatgc tgcccggcca ccgctaagga gaacgctttc gatgaaacgc tccgctataa
    32821 acaacattct cggccataca cggcaattct tttcgcaaca tgacgtacat ctgccgccct
    32881 ttgccagttt ctctcctgct caatggcagc agctcgacac cgccgcgtgg gaggtggttt
    32941 ttgatctcaa actcggctgg gatgttaccg cttttggtgg taacaacttt gcagcacaga
    33001 ggctgacgct gtttaccctg cgtaacggct cagcaaaagg aatgccgtat gtgaaatgtt
    33061 acgccgagaa gatcatgcat gtgcgcgatg cgcaggtcac gccaatgcat tttcactggc
    33121 gtaagcgtga ggacatcatc aatcgcggcg gcggcaattt gattgttgaa ctgtggaatg
    33181 ccgatagcaa cgagcaaaca gttgataccg acgttacggt ggtgatcgac ggctgccgcc
    33241 agactcatgt cgcaggcagt cagttacgcc tgtcccccgg cgaaagtatc tgcctgccgc
    33301 ccggcctgta tcacagcttt tgggcggaaa ccggttttgg cgatgttctg gttggcgaag
    33361 tctcttctgt caatgacgac gaccacgata accacttttt acaaccgctg gaccgctaca
    33421 acctgattga cgaagacgaa ccggcccagc tggtgttgtg taacgaatac cgccagttcc
    33481 tctgaataag gataacgtta tgccgttgat ttctctcgct gaagggctgg cacatgcccg
    33541 cgaacatcac tatgcgttag gtgcgtttaa tgtgctggac tcacactttt tacgcgcctt
    33601 gtttgctgct gccagacaag aacgttcgcc ctttattatc aacattgccg aagtacattt
    33661 taaatatatc tcgctggatt ccctggtcga gacagtgaaa tttgaagcgg cgcgtcaagc
    33721 gattccggtg gtattaaatc tcgatcacgg gctgcatttt gaatcggtgg cgcaagcgtt
    33781 gcggctggga ttcagctcgg taatgtttga tggctccacg ctggggtatg aagagaatat
    33841 ccgccagacg cgggaagtgg tgaaaatgtg ccatgccgtt ggcgtatcgg tagaagctga
    33901 actgggcgcg gtgggtggtg acgaaggcgg tgcgctttac ggtcatgccg atgaatctct
    33961 gtttaccgat ccgcagctgg cgcgtgattt tgtcgaccgt accggcattg acgcgctggc
    34021 cgtggcgatt ggcaatgcgc acggcaaata tcgcggcgaa ccgaaactcg atttcccgcg
    34081 cctcgacgct atccgccagc aggcggctat cccgctggta ttacacggcg gttcagggat
    34141 tagagatgcc gatttccgcc gcgccatcga actgggtatc cacaaaatca atttctacac
    34201 cggcatgtca caagcggcgc tggctgccgt tgaggcccga atggcgcacc gtcagccgat
    34261 gtatgacgag tttgccgagt tactactagc agtggaagcc gccatcagcg atacggttgc
    34321 acaacagatg cgtattttcg gcagtgcggg gcaggcatga tggaacgtaa agggattatc
    34381 gccgcaggca atatgctggt tgaccacgtc caccagatta tccagtggcc agagcgcggc
    34441 tggctggcgg agattaccca cagcgaacgc tccaccggcg gcgcgccgct taacgtcctg
    34501 ctgacactgg cgaaaatgcg caccgggttg ctgttacagg cggtcgggct aatcggtcag
    34561 gacagcgacg gcgattacat tatggcgatg cttgagcaat atcacgtcaa tcgtcagcat
    34621 gtgcagcgca ctacctttgc cccgacgtca atgtcgcagg taatgactga tccaagcggg
    34681 cagcggacgt ttttccattc gccaggtgca aacagacttt tggatcttcc ggcgttcgat
    34741 cagctcgata acaccatgaa gattttccat ctcggctatt tactgttgct cgacagcctg
    34801 gatatgccgg acgatgttta cggcacccgt agcgcacgcc tgctggcgca aatgcgtgat
    34861 actggctttg aaacctcact cgacctggtc tcccgcaagg gcgatccgcg ttatcagccg
    34921 ctggtgttgc ccgcgttgcg ccatctggat tacctggtaa ttaacgaact ggaggccggg
    34981 gagtttagcg ggctggaaat tcggttaccc aacggcgaac cgcatatcgc gcatattgct
    35041 gccgccgcgc gtgaactgtt agatgcgggc gtgcgccagc gtgtggtgat ccactgcccg
    35101 gaaggggcgt ggggagagac cccggaacag tgcggcgtgt ggatcccttc gcaaaagtta
    35161 gaacaacggg aaattatcgg cagcgtcggt gcgggcgatg tattttgtgc cggattttta
    35221 tatggctgcc atgaacgctg gacgcttacc gagagcatca aactggcaca cgcctgcgcg
    35281 cgagcaagcc tgttatgcgc caatgccatc gacggggcga aaacgctgga agagctgcaa
    35341 acagaaatgt cggattaatg cacgtcggca gcgaagacat accccagacc gcgcagagtt
    35401 ttaatcaaca tcggctgatg cgggttgagt tcgattttgc gtcgtaagcg catgattaat
    35461 acgtcgatgg tgcgatcaaa cacctccacg ctgtcgctat gagtgaacgc cagcagctgt
    35521 tcgcggctta atacccgacg ggcattttga gccagggcca gtaacaagcc atattcgccc
    35581 tgagtcagct cgacatgttg ttgtagcgga ctatgcagtt ggcagcgaat ggtatcgaga
    35641 taccagccat tgaatgacag cccggcgttt ttctcctcat taaccgccag cgcgccaacc
    35701 cgtcgcaaca cgctttcagt cgtgcgacca ccacccgtgg ggtgaacggc ttgcagatgt
    35761 aatcatccgc gcccatttcc agcccgacca ccacatcaga ctcactcccc attccggtca
    35821 acatcaccac aggcaatgcc ggacgcagtt tctggatttg ctgcaatacc ctcaggccgt
    35881 tggtatcggg cagcatcata tccagcacca ccagcgcaat gtcaggctcg ccctcaatac
    35941 gcgatatggc ctcctgtccg gtatggctga ctgaaaccgt aaagacatgt tcacttaaca
    36001 cgtcctgcaa cagtgcgcag atagccgtgt catcatccac caccagcacg acgggtttca
    36061 tggttacact ccgcatcatt catctaatta atcattgtgc gtacttcttg tcgaagtgaa
    36121 agcggaaagt aatataaata ataaaaatat aagtcaggtc acattattga tgatagtaat
    36181 aaatatgccg tgcttttccc tcttcagcac aaatgcctaa taaaatggcg cgttccgctc
    36241 acacttccac ttattaaagt cgcaaattcc caattatgat aaccccagct ttaaatgacg
    36301 tattttgcat ggattacata tttacgacac aaatgataaa tggagattta tatgaataac
    36361 tctcggttat tccgtttgag cagaattgtt attgcgttaa ctgccgccag cggcatgatg
    36421 gtaaataccg ctaacgcgaa agaggaagcg aaagccgcca ctcaatatac ccaacaggtt
    36481 aatcagaatt acgccaaatc attaccgttt agcgatcgtc aggattttga cgacgctcag
    36541 cgtggattta tcgctccgct gctggatgaa ggtattctgc gcgatgctaa tggcaaacca
    36601 tattatc
//
LOCUS       DADMGC010000023        36605 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.023, whole genome shotgun sequence.
ACCESSION   DADMGC010000023 DADMGC010000000
VERSION     DADMGC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.023"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..170)
                     /gene="ygdR"
                     /locus_tag="JNZ73_001169"
     CDS             complement(<1..170)
                     /gene="ygdR"
                     /locus_tag="JNZ73_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HAY8731524.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQG"
     gene            complement(308..1021)
                     /locus_tag="JNZ73_001170"
     CDS             complement(308..1021)
                     /locus_tag="JNZ73_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY8731525.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            complement(1090..1779)
                     /gene="mutH"
                     /locus_tag="JNZ73_001171"
     CDS             complement(1090..1779)
                     /gene="mutH"
                     /locus_tag="JNZ73_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HAY8731526.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            2464..2994
                     /gene="rppH"
                     /locus_tag="JNZ73_001172"
     CDS             2464..2994
                     /gene="rppH"
                     /locus_tag="JNZ73_001172"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HAY8731527.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            3007..5253
                     /gene="ptsP"
                     /locus_tag="JNZ73_001173"
     CDS             3007..5253
                     /gene="ptsP"
                     /locus_tag="JNZ73_001173"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY8731528.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            5404..6279
                     /gene="lgt"
                     /locus_tag="JNZ73_001174"
     CDS             5404..6279
                     /gene="lgt"
                     /locus_tag="JNZ73_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAY8731529.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINSELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            6286..7080
                     /gene="thyA"
                     /locus_tag="JNZ73_001175"
     CDS             6286..7080
                     /gene="thyA"
                     /locus_tag="JNZ73_001175"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAY8731530.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            7264..7734
                     /locus_tag="JNZ73_001176"
                     /pseudo
     CDS             7264..7734
                     /locus_tag="JNZ73_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            7725..8288
                     /locus_tag="JNZ73_001177"
     CDS             7725..8288
                     /locus_tag="JNZ73_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY8731531.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            8285..8692
                     /locus_tag="JNZ73_001178"
     CDS             8285..8692
                     /locus_tag="JNZ73_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HAY8731532.1"
                     /translation="MNREKGVSSQALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            8677..9000
                     /locus_tag="JNZ73_001179"
     CDS             8677..9000
                     /locus_tag="JNZ73_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAY8731533.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPLANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            9013..12381
                     /gene="recC"
                     /locus_tag="JNZ73_001180"
     CDS             9013..12381
                     /gene="recC"
                     /locus_tag="JNZ73_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HAY8731534.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            12557..15445
                     /gene="ptrA"
                     /locus_tag="JNZ73_001181"
     CDS             12557..15445
                     /gene="ptrA"
                     /locus_tag="JNZ73_001181"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HAY8731535.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRMADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKTEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            15438..18980
                     /gene="recB"
                     /locus_tag="JNZ73_001182"
     CDS             15438..18980
                     /gene="recB"
                     /locus_tag="JNZ73_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HAY8731536.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEVIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNDDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            18980..20806
                     /gene="recD"
                     /locus_tag="JNZ73_001183"
     CDS             18980..20806
                     /gene="recD"
                     /locus_tag="JNZ73_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HAY8731537.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(20868..22199)
                     /gene="argA"
                     /locus_tag="JNZ73_001184"
     CDS             complement(20868..22199)
                     /gene="argA"
                     /locus_tag="JNZ73_001184"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HAY8731538.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            22431..23684
                     /locus_tag="JNZ73_001185"
     CDS             22431..23684
                     /locus_tag="JNZ73_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HAY8731539.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            complement(23759..23835)
                     /locus_tag="JNZ73_001186"
     tRNA            complement(23759..23835)
                     /locus_tag="JNZ73_001186"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23799..23801),aa:Met,seq:cat)
     gene            complement(23869..23945)
                     /locus_tag="JNZ73_001187"
     tRNA            complement(23869..23945)
                     /locus_tag="JNZ73_001187"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23909..23911),aa:Met,seq:cat)
     gene            24154..25251
                     /gene="mltA"
                     /locus_tag="JNZ73_001188"
     CDS             24154..25251
                     /gene="mltA"
                     /locus_tag="JNZ73_001188"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HAY8731540.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGKTHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            25490..26296
                     /gene="tcdA"
                     /locus_tag="JNZ73_001189"
     CDS             25490..26296
                     /gene="tcdA"
                     /locus_tag="JNZ73_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HAY8731541.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(26347..26790)
                     /gene="csdE"
                     /locus_tag="JNZ73_001190"
     CDS             complement(26347..26790)
                     /gene="csdE"
                     /locus_tag="JNZ73_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HAY8731542.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(26790..27995)
                     /gene="csdA"
                     /locus_tag="JNZ73_001191"
     CDS             complement(26790..27995)
                     /gene="csdA"
                     /locus_tag="JNZ73_001191"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HAY8731543.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            28187..28414
                     /locus_tag="JNZ73_001192"
     CDS             28187..28414
                     /locus_tag="JNZ73_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HAY8731544.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            28765..29682
                     /locus_tag="JNZ73_001193"
     CDS             28765..29682
                     /locus_tag="JNZ73_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HAY8731545.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            29701..30096
                     /locus_tag="JNZ73_001194"
     CDS             29701..30096
                     /locus_tag="JNZ73_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HAY8731546.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            30089..31189
                     /gene="rlmM"
                     /locus_tag="JNZ73_001195"
     CDS             30089..31189
                     /gene="rlmM"
                     /locus_tag="JNZ73_001195"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HAY8731547.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(31233..31964)
                     /gene="fucR"
                     /locus_tag="JNZ73_001196"
     CDS             complement(31233..31964)
                     /gene="fucR"
                     /locus_tag="JNZ73_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HAY8731548.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSSALFHIRLKSHYAHKADIAREALAWIEERMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGYLDEVTHIISDERQVATSLVTA"
     gene            complement(32022..32444)
                     /gene="fucU"
                     /locus_tag="JNZ73_001197"
     CDS             complement(32022..32444)
                     /gene="fucU"
                     /locus_tag="JNZ73_001197"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HAY8731549.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(32446..33865)
                     /gene="fucK"
                     /locus_tag="JNZ73_001198"
                     /pseudo
     CDS             complement(32446..33865)
                     /gene="fucK"
                     /locus_tag="JNZ73_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(33974..35749)
                     /gene="fucI"
                     /locus_tag="JNZ73_001199"
     CDS             complement(33974..35749)
                     /gene="fucI"
                     /locus_tag="JNZ73_001199"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HAY8731550.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHTCGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(35782..>36605)
                     /gene="fucP"
                     /locus_tag="JNZ73_001200"
     CDS             complement(35782..>36605)
                     /gene="fucP"
                     /locus_tag="JNZ73_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HAY8731551.1"
                     /translation="AIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQ
                     TPYMIIVAIVLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQ
                     FCYVGAQTACWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRITGTWLISRFAPH
                     KVLAAYALIAMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYG
                     SSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         8702 a         9136 c         9923 g         8844 t
ORIGIN      
        1 ttaccttgct gatcgtgata actcaccagc ccggtatcat catcaatttc aggttttcca
       61 tcggtcaaaa tcatacggcc atctttggtc gccatgacgt aatcactgga acacccggaa
      121 acagcaaacg ccagtccgac tgcagaaatt attactgccc attttttcat ccggttatcc
      181 tcatttggcc tacctatgat aatagtctgt ttataaatcg acgttatata tcttgaaagc
      241 aggaaaatct taaactgtga gggtaaaagg agtgagtacg ccctcccgca ggagagcgta
      301 cggagtatca gagcggattc tttttgttgc gaatcaagtt gaggctttca accgcaatag
      361 agaagaacat cgcgaagtag atgtaacctt tcggtacgtg gatgtcgaaa ctttccagaa
      421 tcagggtaaa gcccaccaga atcaggaaag agagcgccag catttttacc gaaggatggc
      481 gttcgacaaa atcaccaatc gagcgcgcgg cgaacatcat cacgcctacg gcaatcacca
      541 cggctgccat cataataaac aggtgatctg acagacccac agcggtaatc accgagtcga
      601 ggctgaagat aatatccagc agcataatct gcacgatagc gccgaggaat gatgaaacgc
      661 gtgttttcag cccttcttct tcaccttcaa tggattcgtg gatttccttg ctggctttcc
      721 agataaggaa caagccaccc agaagcagaa tcaaatcacg ggcagaaatt tcctgactga
      781 atattgtaaa aagcggattt gtcaggcgcg taacccaaga gattgatgcc agcagagcca
      841 gacgcataac catggctccc gccaacccca gacggcgcgc atgagcacgt tgtgctgtgg
      901 gaagctttgc caccaccaga gaaaggaaaa taatattgtc gatcccaaga acgatctcca
      961 gcagcgtcag cgtaccgagc gcaagccagg cgttaggatc ggttatccat gcaaataaca
     1021 tctaaaaagc cctgccaaaa aaatgaagcg gtaattatat gcccggaaag cggcaggtca
     1081 aagcgatggc tactggatca gaaaatgacg ggccagtagt gcactggtga aattcttctt
     1141 caaataaaag ccgcgtggca gcgtcagaat ccgttcgccc cgggcaccaa tggcttcggt
     1201 aagcgctttc gcattcgctg ctttcggtct tatctgtaaa tattccccgt gacgagcggt
     1261 gatccgctca acctgaccga gaacaatcat atccattaat tcttcccagt cttcgcgtag
     1321 ctgtcggtct tcctcttcat tcgggctcca cagcaacggt gatcctacgc gacgctgcgc
     1381 cagcgggatg ctgcgctcgc cttcaaccgg tatccacagt acgcgtttga gcttgtggcg
     1441 cacatggctg gtttcccagg tcaccccgct attacccgtt aacggggcaa cacaaacgaa
     1501 tgttgtttcc agcggacgac caagactatc cacagggata gttttaagtt ccacgcccag
     1561 agcagcaaaa tcttgctcag gtttactccc tgcgctggca cctagccaga tctccagtaa
     1621 cacgccaatc cagcccttat cgcgttttaa attctctggc gtaaccagcc cggcaagtgc
     1681 cgccagttct cccaatgtat aaccagaaag ttgctgtgct tgcgctaaca actgttcttc
     1741 agtttcggga ggagagagca gtgggcgagg ttgggacatg tcatgatgcc ttggttaaaa
     1801 aacaaacagc aattatgtgc cgaatattcg cagtgtaact ttttccacat gtgttgttca
     1861 attctctgat ttatattgct ttttaggctg ccggtaatcg agttgcgcaa cactgcaata
     1921 ggttttccaa atctggtcac tgaaaataaa caggatctta caccatgtta tccacagaaa
     1981 gctgggataa ctgtgaaaaa cctccactac tgttttcatt tacagccttg acgtgcgacg
     2041 aaactccagt ttttagtggt tcagtgctta acttagtggc acattctgtg gataaatacg
     2101 gcattggttg atctttcgcc aacccgtaaa tgagaggtgt gacctgggtc acgaattact
     2161 ctttttcatt actcaaatta catgtaatgc attgactaat aatgaatatt ttaaaatgtc
     2221 tatatcgggc ttattcagaa tgctccgggt tttcccgtag taattccgta gttcttcaca
     2281 actctatcca cagaaaaggt gaataaaaac ggctatccac cccttcctct gtttataact
     2341 ctgcacataa ctgtgagtta ttcaattgtt attagtgtgt aatgccctgc agagagtggt
     2401 ttaccgtctt cctggagtat gaaacaatca ttcgtatata aagctttatt ttgaggtagt
     2461 ccggtgattg atgacgatgg ctaccgccca aacgtaggta tcgtgatttg taatcgccag
     2521 gggcaggtaa tgtgggcccg gcgatttggt cagcactcct ggcaatttcc gcaaggcgga
     2581 atcaaccccg gagaatccgc agagcaggcg atgtaccgtg aattgtttga agaagtagga
     2641 ttaagccgca aagacgttcg aatccttgct tcaacgcgta actggttgcg ctacaaatta
     2701 ccgaaacgtt tggtgcgttg ggacacgaag ccggtttgta tcggccaaaa acaaaaatgg
     2761 tttctcttgc agctggtaag cggcgatgca gaaatcaata tgcaaaccag cagtacacca
     2821 gagtttgacg gctggcgatg ggtaagttac tggtatccgg tcagacaggt ggtgtcattt
     2881 aaacgtgatg tctaccgtag ggtaatgaaa gagttcgcga gtgtggtgat gtcactgcag
     2941 gaaaatacgc caaaaccaca aaacgcatct gcttatcgac gtaaaagagg ttaagtcacg
     3001 ccaattatgc tcactcgcct gcgcgaaata gtcgaaaagg tagccagcgc accacgcctg
     3061 aatgaggcgt taaatattct ggttaccgac atctgtcttg cgatggatac cgaggtctgt
     3121 tcggtctacc tggccgatca tgatcgacgt tgttactacc tgatggcgac ccgggggctg
     3181 aaaaaaccac gcggtcgcac tgtaacgctc gcgtttgatg aagggatcgt cggcctggtt
     3241 ggcaggctgg cggaaccgat aaaccttgca gatgcgcaaa agcaccccag cttcaaatac
     3301 atcccctccg taaaagaaga acgtttccgc gcgtttttag gcgtaccaat tattcaacgt
     3361 cgccagttgc ttggtgtact ggtggtacag caacgagagt tgcgccagta tgacgaaagt
     3421 gaagaatcct tcctggtgac gcttgccacc cagatggcag ctattctttc tcagtcgcag
     3481 ttgactgccc tgtttgggca atatcgccag acgcgaatcc gcgcattacc ggcagcacct
     3541 ggtgtggcga ttgccgaagg ctggcaggat gccacgttac ctttaatgga acaggtgtat
     3601 caggcatcaa cgctggatcc ggctctggaa cgcgaacgac tgaccggggc gctggaagaa
     3661 gcggcaaacg agtttcgccg ctacagcaaa cgctttgccg ccggagcaca aaaagaaacg
     3721 gcggctattt tcgatcttta ctcgcacctg ctttcggaca cccggctgcg tcgcgaattg
     3781 tttgccgagg ttgataaagg ctcggtggca gagtgggcgg taaaaacggt cattgaaaaa
     3841 tttgccgaac agtttgccgc gctaagcgat aactatctca aagagcgggc tggcgattta
     3901 cgtgcgctgg gtcagcgatt gctgtttcat cttgatgacg ctaatcaagg gccgaacgcc
     3961 tggccggaac gtttcattct ggtggcagat gaactgtcag cgacaactct tgctgagctg
     4021 ccccaggatc gcttagtcgg tgttgtcgtg cgagatggcg cagccaactc ccatgctgcg
     4081 atcatggtac gtgcgctggg gatccctacc gtgatgggcg cggatattca gccttcggtg
     4141 ctgcatcgta ggacgctgat cgttgatggc tatcgcggtg aattgctggt cgatccggag
     4201 ccggtactgc tgcaagaata tcagcggcta attagtgaag agattgagct tagccgtctg
     4261 gcggaagatg acgtcaattt acccgcgcag ttaaaaagcg gtgagcgtat aaaagtcatg
     4321 ctcaatgctg gtttaagccc ggaacatgaa gaaaaactgg gcagccgtat tgatggcatc
     4381 ggtctttatc gcactgaaat cccattcatg ctgcaaagtg gtttcccgtc ggaagaagaa
     4441 caggtggcgc agtatcaggg gatgctgcaa atgttcaatg ataaacccgt caccttgcgt
     4501 acgctggatg tcggagcaga taagcagctg ccttacatgc cgatcagcga agagaatcca
     4561 tgcctgggtt ggcgtggtat tcgcattacg ctcgatcagc cggagatctt cttgatccag
     4621 gtgcgggcga tgctgcgtgc taatgccgct acgggcaacc tgaatattct gttgccgatg
     4681 gtcaccagcc tcgatgaagt tgacgaagca cgccgcctga ttgaacgtgc cggacgtgaa
     4741 gtcgaggaga tgatcggtta cgaaattccc aaaccacgta tcggcatcat gctggaagtg
     4801 ccgtcaatgg tatttatgct gccgcatctg gcaaagcggg tcgatttcat ctctgttggc
     4861 accaacgatc tgactcaata cattctggcc gttgatcgca acaatacccg ggtggctaac
     4921 atttacgaca gtcttcatcc tgcaatgtta cgagctctgg cgatgatcgc ccgggaagcg
     4981 gaaatacatg gtatcgatct ccgtttgtgc ggtgaaatgg cgggcgatcc catgtgtgtg
     5041 gcaatcctca ttgggcttgg gtatcgccat ctgtctatga acggacgttc tgtagcgcgg
     5101 gtaaaatacc tgctgcggcg cattgatttt gccgaagcag aaaatcttgc gcagcgtagt
     5161 ctggaagcgc aactggcgac cgaagttcgt catcaggttg cagcctttat ggagcgtcgc
     5221 ggcatgggcg ggctgattcg cggagggtta tagcgcggat catatacata tcttttaacg
     5281 gtatccggca accagccagg tccccttgtg ctattattcg cacctttgga gcgcctgaaa
     5341 cctgcggcgc gcatttcaat cgctgttctc tttcagcgaa ataacaagaa cttgtggtga
     5401 cagatgacca gtagctatct gcattttccg gagtttgatc cggtcatttt ctcaatagga
     5461 cccgtggcgc ttcactggta cggcctgatg tatctggtgg gtttcatttt tgcaatgtgg
     5521 ctggcaacac gtcgggcgaa tcgtccgggc agtggctgga ccaaaaatga agttgaaaac
     5581 ttactctatg cgggcttcct cggcgtcttc ctcgggggac gtattggtta tgttctgttc
     5641 tacaatttcc cgcagtttat ggccgatccg ctgtatctgt tccgtgtctg ggacggcggc
     5701 atgtctttcc acggcggcct gattggcgtt atcgtggtga tgattatctt cgcccgccgt
     5761 actaaacgtt ccttcttcca ggtctctgat tttatcgcac cactcattcc gtttggtctt
     5821 ggtgccgggc gtctgggcaa ctttattaac agtgaattgt ggggccgcgt tgacccgaac
     5881 ttcccgtttg ccatgctgtt cccaggctcc cgtacagaag atattttgct gctgcaaacc
     5941 aacccgcagt ggcaatccat tttcgacact tacggtgtgc tgccgcgcca cccatcacag
     6001 ctttacgaac tgctgctgga aggtgtggtg ctgtttatta tcctcaacct gtatattcgt
     6061 aaaccgcgcc caatgggagc tgtctcaggc ttgttcctga ttggctacgg cgcgtttcgc
     6121 atcattgttg agtttttccg ccagcccgac gcgcagttta ccggtgcctg ggtgcagtac
     6181 atcagcatgg ggcaaattct ttccatcccg atgattgtcg cgggtgtgat catgatggtc
     6241 tgggcatatc gtcgcagccc acagcaacac gtttcctgag gaaccatgaa acagtattta
     6301 gaactgatgc aaaaagtgct cgacgaaggc acacagaaaa acgaccgtac cggaaccgga
     6361 acgctttcca tttttggtca tcagatgcgt tttaacctgc aggatggatt cccgctggtg
     6421 acaaccaaac gttgccacct gcgttccatc atccacgaac tgctgtggtt cctgcagggc
     6481 gatactaaca ttgcttatct acacgaaaac aatgtcacca tctgggacga atgggccgat
     6541 gaaaatggcg acctcgggcc agtgtatggt aaacagtggc gcgcctggcc aacgccagat
     6601 ggtcgtcata ttgaccagat tactacggta ctgaaccagc tgaaaaacga cccggattcg
     6661 cgccgcatta ttgtttcagc gtggaacgta ggcgaactgg ataaaatggc gctggcaccg
     6721 tgccatgcat tcttccagtt ctatgtggca gacggcaaac tctcttgcca gctttatcag
     6781 cgctcctgtg acgtcttcct cggcctgccg ttcaacattg ccagctacgc gttactggtg
     6841 catatgatgg cgcagcagtg cgatctggaa gtgggtgatt ttgtctggac cggtggcgat
     6901 acgcacctgt acagcaacca tatggatcaa actcatctgc aattaagccg cgaaccgcgt
     6961 ccgctgccga agttgattat caaacgtaaa cccgaatcca tcttcgacta ccgtttcgaa
     7021 gactttgaga ttgaaggcta cgatccacat ccgggcatta aagcgccggt ggctatctaa
     7081 ttacgaaaca tactgccaga gccgacgcca gtgtgcgtcg gtttttttac cctccgttaa
     7141 attcttcgag acgccttccc gaaattttgc aacgtcctgc aacggcgtaa atagtccgga
     7201 agatgcgccg aagaaataga aactggccct cgtctttttt tctcctcgct ccatactgcc
     7261 ggcatgaaaa cacaacgtgg ttatacgctg attgaaacgc tggtcgcgat gctgattttg
     7321 gtcatgctaa gcgcaagtgg actctatggc tggcaatact ggcagcagtc gcaacggctt
     7381 tggcaaaccg ccagctaggc gcgggactat ttgctctatt tacgtgaaga tgccaactgg
     7441 cataaccgcg accacagtat cagcgttatc agggagggga cgttatggtg ccttgtgagt
     7501 tccgctgctg gggccaatac ctgtcatggc agttcaccat tggtctttgt gccacgctgg
     7561 cccgaagtcg aaatgagcga cctgacacct tcgcttgctt tctttggcct gcgcaatacc
     7621 gcatgggccg ggcatattcg cttcaaaaac tcaacgggcg agtggtggct ggtggtttcg
     7681 ccgtggggaa gactccggct ttatcagcaa ggagaaacag aaggatgcct gtaaaagagc
     7741 aaggtttttc tctgctggaa gtgttgattg ctatggcgat cagtagcgta ttgttgctgg
     7801 gggctgcacg ctttctgcct gcgttacagc gtgaaagttt aacgagcact cgtaagctgg
     7861 cactggaaga tgaaatctgg ctgcgggtat ttaccgtcgc gaagcatctc cagagggcgg
     7921 gttattgtca tggcagctgt accggcgaag ggctggaaat tgtcggacag ggtgactgta
     7981 tcattgtgca gtgggatgcg aacagtaacg gtatctggga tcgcgaaccg gtaaaagagt
     8041 ccgaccagat tggatttcgt ctgaaggagc atgtgctgga aacgctacgc ggtgcgacat
     8101 cctgtgaagg taagggctgg gataaagtca ctaatccgga tgccatcatt atcgacactt
     8161 ttcaggtcgt acgtcaggat gtcagcggct tctcgccggt gttgacggtt aatatgcgtg
     8221 ctgccagtaa gtctgaaccg caaaccgtgg tggatgccag ctatagcgtg acaggattca
     8281 acctgtgaac cgcgaaaaag gtgtttcgtc acaggctctg gtcctgatgc tgctggtttt
     8341 gggtagcttg ctattgcaag gaatgagtca gcaggatcgc agttttgcct ctcgcgtgag
     8401 catggaaagt cagtcattgc gccgccaggc catcgttcag tcggcgctgg cgtggggaaa
     8461 aatgcactcc tggcagacgc agccagcagt tcagtgctcg cagtacgctg gaaccgatgc
     8521 ccaggtttgt ttgcgtttac tggcagataa tgaagcctta ttgattgccg gttatgaagg
     8581 cgtttcgttg tggcgaacag gcgaagtcat tgatggaaaa attgtttttt cgccacgcgg
     8641 ctggagcgat ttttgtccgc tgaaagaggg ggcattatgt cagcttccct gaagaatcaa
     8701 caaggcttta gcctgccgga ggtaatggtt gcgatggtgc tgatggtgat gattgtcact
     8761 gcgttatcgg gtatccagcg aacattaatg aacagtctcg ccagcagaaa ccagtaccaa
     8821 cagctctggc ggcatggttg gcagcaaacg caactgcgcg cgatttcgcc acttgccaac
     8881 tggcaggtca accgaatgca gacatcgcag gcgggatgtg tcagcatcag cgttacgcta
     8941 gtttcacccg ggggcagaga aggcgagatg acccgcctgc attgcccgaa tcgtcagtag
     9001 tcaggagccg ctatgttaag ggtctaccat tccaatcgtc tggacgtgct ggaagcgttg
     9061 atggagttta ttgtcgaacg cgaacggctg gacgatcctt tcgaaccaga gatgattctg
     9121 gtgcaaagta ccggtatggc gcagtggctg caaatgaccc tgtcgcaaaa gtttggtatt
     9181 gcggcaaaca ttgattttcc gctgccagcg agctttatct gggatatgtt cgtccgggtg
     9241 ttaccggaaa tccccaaaga gagcgccttt aacaaacaga gcatgagctg gaaactgatg
     9301 actctgctgc cgcaattgct ggagcgcgaa gactttaccc tgttgcggca ttatctgact
     9361 gacgatagcg acaagcgaaa actgttccag ctttcctcaa aagcggcgga cctgtttgac
     9421 cagtatctgg tctatcgtcc ggactggctg gcacagtggg aaacaggaca tttggttgaa
     9481 gggctgggag aagcacaggc ctggcaagcc ccgttgtgga aggcgctggt ggaatatacc
     9541 catgaactcg gacaaccgcg ctggcaccgc gcgaatctct atcagcgctt tatcgaaacg
     9601 ctggagtccg cgacgacctg cccgccgggg ttaccttcgc gcgtctttat atgcggtatt
     9661 tccgcgttac cgcctgttta tctccaggcg ctccaggcgc tgggtaaaca tattgaaatc
     9721 catctcctgt ttaccaaccc ctgccgttat tactggggcg atattaaaga tcctgcttat
     9781 ctggcgaaac tgctgactcg tcagcgtcga cacagttttg aagatcgcga attgccgcta
     9841 tttcgcgata gcgaaaatgc cggacagctc tttaacagcg atggtgaaca ggatgtcggc
     9901 aacccgctgc tggcctcatg ggggaagctt gggcgcgact acatttatct cctttctgac
     9961 ctggagagca gccaggagct ggacgctttt gtcgatgtga cgccagataa cctgctgcat
    10021 aacattcagt ctgacattct ggaactggaa aaccgcgccg ttgcgggtgt gaacatcgaa
    10081 gagttttccc gtagcgataa caaacgcccg cttgatccac tggatagcag tatcacattc
    10141 cacgtttgcc atagcccgca gcgtgaagtt gaagttttac acgatcgctt gctggcgatg
    10201 ctggaggaag ccccgacact tactccgcgc gacatcatcg tgatggtggc tgatatcgac
    10261 agctacagtc cgtttattca ggcagtgttt ggtagcgcac ctgcggatcg ttacctgcct
    10321 tacgccattt ccgaccgtcg tgcgcggcag tcacatccgg tactggaagc gtttatcagc
    10381 ctgttatcac tgcctgacag tcgctttgtg tcagaggatg tgctggcgct gctggatgtg
    10441 ccggtgctgg cggcgcggtt tgacatcacc gaagaagggc tgcgttattt acgccagtgg
    10501 gtcaacgaat ccggcattcg ttgggggata gatgacgaca acgttcgcga gctggaactc
    10561 cccgccaccg gacaacacac ctggcgattt ggcctgacgc gtatgttgct gggctacgcg
    10621 atggagagcg cgcagggcga gtggcaatcg gttctacctt atgatgaatc gagcggctta
    10681 attgcagaac tggtggggca tctggcttca ctgctaatgc agctaaacat ctggcgtcgc
    10741 gggctggcac aggagcgtcc gctagaagag tggttgccgg tttgtcgcga tatgctcaac
    10801 gccttcttcc tgccggatgc ggaaaccgaa gcggcgatga cgctgatcga acaacaatgg
    10861 caggcgatta tcgccgaagg tttaggtgcg cagtatggcg acgcggtgcc gctgtcacta
    10921 ttgcgtgatg aactggcaca gcgtctggat caagaacgta tcagccagcg ttttctcgcc
    10981 ggaccggtta atatttgtac tctgatgcca atgcgttcaa ttccgttcaa agtggtttgc
    11041 ctgctgggaa tgaacgacgg cgtttatcca cgtcagcttg cgccattggg ctttgacctg
    11101 atgagccaga aaccgaagcg tggcgaccgt agccgtcgcg atgacgaccg ctacctgttc
    11161 ctggaagcgt taatttccgc gcagcaaaaa ctctatatca gctatatcgg gcgttccatt
    11221 caggataaca gtgaacgttt cccgtcggta ctggtgcagg aactgatcga ctacatcggg
    11281 caaagtcatt atctaccggg cgatgaagcg ctcaactgtg atgaaagcga ggcaagggta
    11341 aaagcgcatc ttacttgcct ccatacccgg atgccgtttg atccgcaaaa ctaccagcca
    11401 ggcgaacgac aaagctatgc tcgtgaatgg ctacctgcgg ccagccaggc tggtaaagca
    11461 cattctgaat ttgttcagcc gctgccgttt accttaccgg aaaccgtgcc gctggaaacg
    11521 ctacaacgat tctgggcaca tccggtgcgg gcgttcttcc agatgcgttt gcaggtgaac
    11581 ttccgtactg aagacagcga aatcccagac accgagccat ttattctgga aggacttagc
    11641 cgttatcaaa tcaatcagca gttattgaat gcactggttg agcaggatga tgccgaacgc
    11701 ttgttccgcc gcttccgggc ggcaggggat ttaccgtatg gcgcttttgg tgaaattttc
    11761 tgggaaacac agtgccagga gatgcagcag cttgccgaca gagtcattgc ctgtcgccag
    11821 ccggggcaga gtatggaaat tgatctcgcc tgcaacggtg tgcagataac tggctggttg
    11881 ccgcaggtgc agccggatgg cctgttgcgc tggcgtccct ctttattaag tgtggcgcag
    11941 ggaatgcaac tttggctgga acaccttgtc tactgtgcca gcggtggtaa tggcgaaagt
    12001 cgcctttttc tacgcaaaga cggtgagtgg cgttttccgc cgcttgcagc cgaacaggct
    12061 ttgcattacc tctcacaact gattgagggg tatcgtgaag gaatgtccgc gccattgctg
    12121 gtgttacctg aaagtggcgg cgcgtggcta aaaacctgtt atgacgcgca aaacgatgcc
    12181 atgctggatg acgattctac gttgcaaaaa gcccgtacga aattccttca ggcttacgaa
    12241 ggcaacatga tggtgcgtgg cgaaggtgat gatatctggt atcaacggct ctggcggcaa
    12301 ttaacaccag agacaatgga ggccatcgtt gaacagtcgc aacgtttcct gttaccgctg
    12361 tttcgcttta atcagtcatg agtgttgtat aaaaattgcg caatctatcc gcttacttta
    12421 tgatgcgcac cagtcacgga ctgatggtta tataaacata ggctgactcg tgcagcacaa
    12481 gattaaattc tggcagatga tttgcgttaa cctgttgaat ctgtccagaa aataaagttg
    12541 attatgaggt ccgtgaatgc cccgcagcac ctggttcaaa gcattattgt tgtttgttgc
    12601 cctctgggca ccattaagtc aggcagaaac gggatggcag ccgattcagg aaaccatccg
    12661 taaaagtgat aaagataacc gccagtatca ggctatacgt ctggataacg gtatggtggt
    12721 cttgctggtt tctgatccgc aggcagttaa atcgctctcg gcgctggtag tgcccgttgg
    12781 gtcgctggaa gatcccgagg cgtaccaggg gctggcacat taccttgaac atatgagtct
    12841 gatggggtcg aaaaagtacc cgcaggctga cagtctggcc gaatatctca aaatgcacgg
    12901 cggtagtcac aatgccagca ctgcgccgta tcgcacggct ttctatctgg aagttgagaa
    12961 cgacgccttg cccggtgcgg tagaccgcat ggccgatgct attgcagaac ccttgctcga
    13021 caagaaatac gccgaacgtg agcgtaatgc agtgaacgct gaattaacca tggcgcgtac
    13081 gcgtgacggg atgcgcatgg cacaggtcag cgcagaaacc attaacccgg cacaccccgg
    13141 ttcaaagttt tctggtggta acctcgaaac tttaagcgac aaacctggta atccggtgca
    13201 gcaggcgctg aaagatttcc acgagaagta ctattccgcc aatttgatga aggcggttat
    13261 ttacagtaat aaaccgctgc cggagttggc aaaaatggcg gcggacacct ttggtcgcgt
    13321 gccgaacaaa gagagcaaaa aaacggaaat caccgtgccg gtagtcaccg acgcgcaaaa
    13381 gggcattatc attcattacg tccctgcgct gccgcgtaaa gtgttgcgcg ttgagtttcg
    13441 catcgataac aactcagcga agttccgtag taaaaccgat gaattgatta cctatctgat
    13501 tggcaatcgc agcccaggta cactttctga ctggctgcaa aagcagggat tagttgaggg
    13561 cattagcgcc aactccgatc ctatcgtcaa cggcaacagc ggcgtattag cgatctctgc
    13621 gtctttaact gataaaggcc tggctaatcg cgatcaggtt gtggcggcaa tttttagcta
    13681 tctcaatctg ttacgtgaaa aaggcattga taaacaatac ttcgatgaac tggcgaatgt
    13741 gctggatatc gacttccgtt atccgtcgat cacccgtgat atggattacg tcgaatggct
    13801 ggcagatacc atgattcgcg ttcctgttga gcatacgctg gatgcagtca atattgccga
    13861 tcggtacgat gctaaagcag taaaggaacg tctggcgatg atgacgccgc agaatgcgcg
    13921 tatctggtat atcagcccga aagagccgca caacaaaacg gcttactttg tcgatgcgcc
    13981 gtatcaggtc gataaaatca gcgcacaaac tttcgccgac tggcagaaaa aagccgccga
    14041 cattgcgctc tctttgccag agcttaaccc ttatattcct gatgatttct cgttgattaa
    14101 gtcagagaag aaatacgacc atccagagct gattgttgat gagtcgaatc tgcgcgtggt
    14161 gtatgcgcca agccgttatt ttgccagcga gcccaaagct gatgtcagcc tgattttgcg
    14221 taatccgaaa gccatggaca gcgcccgcaa tcaggtgatg tttgcgctca atgattatct
    14281 cgcagggctg gcgcttgatc agttaagcaa ccaggcgtcg gttggtggca taagtttttc
    14341 caccaacgct aacaacggcc ttatggttaa tgctaatggt tacacccagc gtctgccgca
    14401 gctgttccag gcattgctcg aggggtactt tagctatacc gctacggaag agcagcttga
    14461 gcaggcgaag tcctggtata accagatgat ggattccgca gaaaagggta aagcgtttga
    14521 gcaggcgatt atgcccgcgc agatgctctc gcaagtgccg tacttctcgc gagatgaacg
    14581 gcgtaaaatt ttgccctcca ttacgttgaa agaggtgctg gcctatcgcg acgccttaaa
    14641 atcaggggct cgaccagagt ttatggttat cggcaacatg accgaggccc aggcaacaac
    14701 gctggcacgc gatgtgcaaa aacagttggg cgctgatggt tcagagtggt gtcgaaacaa
    14761 agatgtagtg gtcgataaaa aacaatccgt catctttgaa aaagccggta acagcaccga
    14821 ctccgcactg gcagcggtat ttgtaccgac tggctacgat gaatacacca gctcagccta
    14881 tagctctctg ttggggcaga tcgtacagcc gtggttctac aatcagttgc gtaccgaaga
    14941 acaattgggc tatgccgtgt ttgcgtttcc aatgagcgtg gggcgtcagt ggggcatggg
    15001 cttccttttg caaagcaatg ataaacagcc ttcattcttg tgggagcgtt acaaggcgtt
    15061 tttcccaacc gcagaggcaa aattgcgagc gatgaagcca gatgagtttg cgcaaatcca
    15121 gcaggcggta attacccaga tgctgcaggc accgcaaacg ctcggcgaag aagcatcgaa
    15181 gttaagtaaa gatttcgatc gcggcaatat gcgcttcgat tcgcgtgata aaatcgtggc
    15241 ccagataaaa ctgctgacgc cgcaaaaact tgctgatttc ttccatcagg cggtggtcga
    15301 gccgcaaggc atggctattc tgtcgcagat ttccggcagc cagaacggga aagccgaata
    15361 tgtgcatcct gaaggctgga aagtgtggga gaacgtcagc gcgttgcagc aaacaatgcc
    15421 cctgatgagt gaaaagaatg agtgatgtcg ccgagacact agatcctttg cgcttgccct
    15481 tacagggcga gcgcctgatt gaagcctctg ccggcacagg caaaaccttt acgattgcgg
    15541 cgctctattt gcgcctgtta cttggactag gcggttccgc cgcctttccc cgcccgctga
    15601 tcgttgaaga actgctggtg gtcaccttta ccgaggctgc cacggcagaa ttgcgcggtc
    15661 gtatccgtag caatatccac gagttgcgca tcgcctgtct gcgtgaaacc accgacaatc
    15721 cactgtacga acgcctgctg gaagagatcg acgataaagc gcaagccgcg cagtggttgt
    15781 tgttagccga acggcagatg gatgaagcgg cagtctttac tattcacggc ttttgccagc
    15841 gcatgctcaa cctgaatgcc tttgaatccg gcatgctgtt tgagcagcag ctgattgaag
    15901 atgagtctct gctacgctac caggcctgcg ccgatttctg gcgtcgccac tgctacccgc
    15961 tgccgcgtga aatagcccag gtcgtctttg aaacctggaa agggccgcag gcgttgctgc
    16021 gcgatattaa tcgttatctg caaggcgaag cgccggttat caaagcaccg ccgcccgatg
    16081 atgaaacgct ggcttcccgc cacgcgcaaa ttgtggcgcg tattgatacg gtaaaacagc
    16141 agtggcgcga cgcagtgggt gaactggatg cgctgatcga atcttctggt attgatcgac
    16201 gcaagtttaa ccgtagcaat caggctaaat ggatcgaaaa gatcagcgct tgggcagaag
    16261 aagagacaaa cagttatcag ttgccggagt cgctggaaaa attctcccag cgtttcttag
    16321 aagatcgcac gaaggccggg ggggaaaccc cgcgacatcc actgtttgag gtgatcgatc
    16381 aactgcttgc agaaccattg tcgatccgcg atctggtgat cacccgagca ttggctgaga
    16441 tccgcgaaac agtagcgcgt gaaaaacgcc gccgtggcga attgggtttt gatgacatgt
    16501 taagtcggct cgattccgcg ctgcgtagcg aaagcggcga ggtgttggca gcggcgatcc
    16561 gtacgcgatt cccggtggca atgatcgatg aatttcagga taccgacccc cagcagtacc
    16621 gaatttttcg ccgtatctgg caccatcagc cggaaaccgc attgttgctg attggcgacc
    16681 cgaagcaggc catatatgca ttccggggtg cggatatctt cacttatatg aaggcgcgta
    16741 gtgaagttca cgcccactac actttagaca ccaactggcg ctccgcacct ggaatggtga
    16801 acagtgtgaa caagcttttc agccagactg atgacgcgtt catgtttcgc gaaataccgt
    16861 ttattccagt gaaatcagcc gggaaaaatc aggcgttacg ttttgtattt aaaggtgaaa
    16921 cgcagcctgc gatgaaaatg tggctgatgg aaggcgaaag ctgcggcgtt ggcgactatc
    16981 aaagtaccat ggcgcaggta tgtgctgcgc aaatccgcga ctggctacaa gccggacagc
    17041 ggggcgaagc gttgctgatg aacgacgacg acgcgcgtcc ggtgcgtgct tcggacatca
    17101 gcgtgctggt gcgcagccgc caggaggccg cccaggtgcg cgatgcctta acgttgctgg
    17161 aaatcccttc cgtttacctt tcgaaccgcg acagtgtttt tgaaactctg gaagcgcagg
    17221 aaatgctttg gttgttgcag gcggtgatga cgcccgaacg tgagaacacc ctgcgcagtg
    17281 cgctggcaac gtcaatgatg gggctgaatg cgctggatat tgaaacgctg aataatgacg
    17341 aacatgcgtg ggatgcggta gtcgaagagt tcgatggtta tcggcaaatc tggcgcaaac
    17401 gtggcgttat gccgatgctg cgggcgctga tgtcggcgcg taacattgca gaaaacttgc
    17461 tggcaacggc aggcggtgag cggcgtctta ccgatatctt gcatatcagc gaactgcttc
    17521 aagaagccgg aacgcagctg gaaagtgaac atgcgctggt acgctggtta tcgcaacata
    17581 tcctcgagcc agacagtaat gcctccagcc aacaaatgcg cctcgaaagt gataaacatc
    17641 tggtgcagat tgtcacgatc cataaatcga aagggctgga atatccgctg gtctggttgc
    17701 catttattac caatttccgc gtccaggatc aggcgtttta tcacgatcgc cactcgtttg
    17761 aggcagtttt ggatcttaat gctgcgccag aaagcgtcga cctcgcggag gtcgaacgtc
    17821 tggcggaaga tctgcgtttg ctttacgtgg cgctgacgcg ttcggtttgg cattgcagtc
    17881 tcggcgttgc accgctggtg cgccgtcgtg gcgataaaaa aggtgacacc gacgtccacc
    17941 aaagtgcgct cgggcgtttg ctgcaaaaag gggaaccgca agatgcggca gggcttcgca
    18001 cctgtattga agcgttatgc gatgatgata ttgcctggca aacggcacaa actggtgata
    18061 accagccctg gcaggttaat gatgctttaa ctgcagaact gaatgcgagg acactacaac
    18121 gattgcccgg cgataactgg cgtgtcacca gctactccgg cttgcagcag cgtggtcacg
    18181 gtatcgctca ggatctgatg ccacggctgg atgttgatgc cgcaggcgtg gtcagcgtcg
    18241 ttgaagaacc gacgttaaca ccgcatcagt tcccgcgcgg tgcgtcaccg gggacattct
    18301 tgcacagttt gtttgaagac ctcgatttta cccagccggt tgacccgaac tgggtacagg
    18361 aaaaactgga actcggcggc tttgaatcgc agtgggaacc ggtgttgacc gagtggatca
    18421 cggctgtcct ccaggcacct ctcaatgaaa cgggtgttag cctgagtcag ctttccgacc
    18481 gcgataaaca ggtggagatg gagttttacc tgccgattag cgaaccgctc atcgccagcc
    18541 agcttgatgc actaattcgc cagtttgacc cgctatccgc tggctgcccg ccgctggagt
    18601 tcatgcaggt acgcggcatg ttaaaaggct ttatcgacct ggtattccgc cacgaagggc
    18661 gttattacct gctcgactat aaatccaact ggctgggtga agacagttcg gcttacaccc
    18721 aacaggctat ggcagcagcg atgcaggcac accgctatga tctgcaatat cagctttata
    18781 ccctggcact gcatcgttat ctgcgccatc gcattgctga ttacgactat gatcttcact
    18841 ttggcggcgt tatctatctg ttcctgcgtg gcgttgataa agaacatccg caacaaggga
    18901 tttacacaac ccgacccaac gccgggttga ttgccctgat ggatgagatg tttgccggta
    18961 tgaccctgga ggaggcgtaa tgaaattgca aaagcaatta ctggaagctg tggagcacaa
    19021 acagctacgc ccgctggatg tgcaatttgc cctgaccgtg gcgggagatg aacatcctgc
    19081 tgtcaccctc gcggcggcac tgttaagtca tgatgccgga gagggacacg tctgtttgcc
    19141 gctttcacga ctggaaaata acgaggcgtc gcatccgctg ttggcgacct gtgtcagtga
    19201 aatcggtgag ctacaaaatt gggaagaatg cttgctggct tctcaagcgg tcagcagggg
    19261 agatgaaccc acgccgatga tcctctgtgg cgatcgtctt tatttgaatc gcatgtggtg
    19321 taacgagcgg acggtagcgc gcttcttcaa tgaggtgaat cataccattg aggttgatga
    19381 agctctactg gcgcaaaccc tggacaaact ttttccagta agcgatgaaa ttaactggca
    19441 aaaagttgca gcggcagtgg cgctgacgcg gcggatctcg gtgatttccg gcggcccagg
    19501 caccggtaaa acgaccaccg tggcgaagtt gctggcagcg ttaattcaaa tggccgacgg
    19561 cgaacgctgc cgtatccgtc tggctgcacc aacgggtaaa gctgccgcgc gcttaaccga
    19621 atctctcggc aaggctttgc gacagttacc gctgaccgat gaacaaaaga aacgcattcc
    19681 ggaagatgcc agcactttgc accgattgct gggcgcgcag ccgggtagcc agcgtttacg
    19741 tcatcatgcc ggtaacccgc tgcatcttga tgtgctggtg gtagatgaag cgtcaatgat
    19801 cgatctgcct atgatgtcga gactgatcga cgccttgcca gatcatgcgc gagtgatctt
    19861 tctcggcgat cgtgatcaac tggcctcggt tgaggctggg gctgtgctgg gcgatatctg
    19921 cgcttatgcc aacgcgggct ttaccgccga gcgtgccagg cagctaagcc gcctgacggg
    19981 gactcacgtt ccggcaggaa ccggcacaga agcggcatct ttgcgcgaca gtctctgcct
    20041 gctgcaaaaa agctatcgtt tcggcagcga ttctggcatt ggtcagttag ctgcggcgat
    20101 caaccgtggt gataaaacgg cagtgaaaac cgtttttcag caggatttta ctgatatcga
    20161 aaaacgactt ttacagagcg gcgaagatta tattgcgatg cttgaggaag ctcttgcggg
    20221 ttacggacgt tatctggatc tgctgcaagc gcgtgccgag ccggatttaa tcattcaggc
    20281 gttcaatgag taccagcttt tgtgcgccct gcgggaaggg ccgtttggcg tggctggact
    20341 gaatgagcga attgagcagt ttatgcaaca gaagcgcaaa attcatcgtc atccgcactc
    20401 tcgttggtac gaaggccgac cggtgatgat tgcccgtaat gacagcgcgc ttgggttgtt
    20461 taatggcgat atcggtattg cgctggatcg cgggcagggg acgcgcgtct ggtttgcgat
    20521 gccggacggc aatattaagt ctgtgcaacc gagtcgcttg ccagagcacg aaactacgtg
    20581 ggcgatgacg gtacataaat cgcagggatc ggagttcgac catgcggcgt tgattttacc
    20641 gagccaacgc acgccggtag taacgcgaga gctggtatac accgcggtga cccgcgcgcg
    20701 tcgtcgtctg tcgctgtatg ccgatgagcg tatattaagt gcggcaatcg ccactcgtac
    20761 tgagcggcgc agtggtctgg cggcgttgtt tagttcgcgg gaataaacgt aattgccgga
    20821 tgcgacgcac gagtgttacg cctgccgcat ccggtgattt tcatcgctta ccctaaatcc
    20881 gccatcaaca ctttggattt acgctggtag ttgtacaact gctttttgct ctcgggcagt
    20941 aaatcaatat ccaccggggt aaatccacgt tcctggaacc agtgaatact gcgcgtggtc
    21001 agcacaaaca atttgcttaa gccgctctgc ttcgcctgag cggcaatgcg ttccagcaga
    21061 acctcgcccc gtgatgaact gcggtaatcc gggtgaactg ccacacaggc catttcccca
    21121 atcttctctt ccgggaacgg atagagcgcg gcgcaggcaa tagtcgtgtt atcgcgctga
    21181 ataatggtga atttgtcgat ttccatctcc agctgctcgc gagaacggcg caccagaata
    21241 ccttgctgct ccagcgggcg aatcaactcc agaataccgc caatatcgtt gattgttgcg
    21301 cgacgaatct gctcggcgct ttccatcaca atctgcgtac cgataccgtc gcgtgagaac
    21361 aactcttgca acagcgcgcc atcttcctga taactgatta aatgacagcg gcgcacgccg
    21421 ctgcggcagg ctttcactgc gccacgcaaa aagcgcaccg taccggagtt gtaatcgcct
    21481 ttctcttcct gggcttctac ccgtgcttgc gcttcgttag ggaaaagttc ggagacaata
    21541 tcaccgtcgt cattagtaac gccctgggaa gagcaaaaac caatcatctt ttcagctttc
    21601 agtttgatgg ctagttgagt ggcaatctct tcagaggtca gattaaagct ctcgccagtg
    21661 actgaaacag cgaccggccc cattagcact attgcaccgc tttccagttg acgatggatc
    21721 gcgtcttcat caatccgccg gatacgcccg ctatggcagt aatccacgcc gtcatcgacg
    21781 cccagcggct gggcaataat aaaattgcca ctaacgacgt tgatatgcgc gccctgcagc
    21841 ggcgtgttat tgagactcat tgacaggcga gcagtaatat ccagttgcaa tgttcccgca
    21901 gcctgcttca ccagttccag tgttttggcg tcggtcacac gtatattctt gtgatacagc
    21961 ggttcgtggt gatgcgcagc cagatttgcg tcgatctgcg gacgtgcgcc atagaccacc
    22021 accagacgga tgccgaggct gtgcaacaac ccgatatcat taacgatact ggagaaattc
    22081 tcatgctcaa tggcttcacc gccgagcatg atgacaaacg tttttccccg gtgggtattg
    22141 atatagggaa ccgaatggcg gaatccctcg accagctcgg ttttacgttc ctttaccacg
    22201 gcacacccct ttgcatgatt atttgaaatt attgtatttt tattctgttt tttcgcaggg
    22261 tgcaagtgta aattttatgc gagagcgact tttttatcag taatgccgtg aatataaaaa
    22321 gaatgtttac cgtttattag atgacagatt atgcgtcttt cgctaaagtt tccggtcaaa
    22381 ttagtcgttt acttgttaca cagcttagat ttcttttgct cgggagaggc atgtcaggat
    22441 ccaacactgc aatcagccgt cgtcgtttac tgcaaggcgc gggcgctatg tggctattga
    22501 gcgtaagtca ggtcagcctg gctgcggtta gccaggtcgt ggcggtgcgc gtctggcctg
    22561 cgtccagcta cacccgcgtg acggtagaat caaatcgtca gctgaaatat aagcagttcg
    22621 cgttgagtaa ccctgaacgc gtggtggtgg atatcgaaga tgtaaacctg aactcggtac
    22681 tcaaggggat ggcggcacaa attcgtgcag acgacccgtt catcaagtcg gcgcgcgtcg
    22741 ggcaatttga cccgcaaacc gtacgcatgg tttttgaatt aaagcaaaac gtaaaaccgc
    22801 agctgtttgc ccttgcgccg gtcgccgggt ttaaagagcg tctggtgatg gacctctatc
    22861 cggccaatgc acaggatatg caggacccgc tgctggcgct gctggaggat tacaacaaag
    22921 gcgacctcga aaagcaggtg ccgccagcgc aaagtggtcc acaaccgggt aaagcagggc
    22981 gcgatcgtcc gattgtcatt atgcttgacc ctggccacgg tggcgaagat tccggtgcgg
    23041 tggggaaata caaaacgcgc gaaaaagatg tggtattgca aatagctcgc cgtttgcgct
    23101 ctctgatcga gaaagagggc aatatgaagg tgtacatgac gcgcaatgaa gacatcttca
    23161 ttccgttgca agtgcgcgta gcaaaagccc agaaacagcg tgctgacctg tttgtctcta
    23221 tccatgccga cgcctttacc agtcgtcagc cgagcggttc ctcggtgttt gcgctctcaa
    23281 ccaaaggcgc aaccagtact gcggcaaaat atctggcaca aacccagaac gcctcggact
    23341 tgattggtgg cgtgagcaaa agcggtgacc gctatgtcga ccacaccatg ttcgatatgg
    23401 tgcagtcgct gactattgct gacagcctta agtttggtaa agcggtactg aataagctcg
    23461 gaaaaatcaa caagctgcat aaaaatcaag ttgaacaggc cgggtttgcc gtactaaagg
    23521 caccagatat tccctccatt ctggtcgaaa cggcgtttat cagtaacgtt gaggaagagc
    23581 gtaaactgaa aacggcgact ttccagcagg aagttgcgga gtctattctt gcggggatta
    23641 aagcgtattt tgccgatggg gcaacgctgg cgagaagggg atgataaaaa ggcgctgaat
    23701 ggcgcttttt ttatgggaga tcagaaacaa aaaaacaccc gttagggtgt tcaataattg
    23761 gttgcggggg ccggatttga accgacgacc ttcgggttat gagcccgacg agctaccagg
    23821 ctgctccacc ccgcgtcacc gtactgcttt tacttcatca aatttgattg gttgcggggg
    23881 ccggatttga accgacgacc ttcgggttat gagcccgacg agctaccagg ctgctccacc
    23941 ccgcgtccgt ggatgcgcac tatactccgc tcgcgttttc atgcaacctt ttttttaccc
    24001 cgatcacgga tttagcatga ttttgaacaa aaacaaacca ctaaagctta ttttttgtgc
    24061 gaaatttgcg tcagggagag gctgctcatt tcattaaacg cggcggtttg ttatcttcgt
    24121 tgcgccttat tttttaacct gaagaagaga acaatgaaag gacgttgggt aaagtacctt
    24181 cttatgggca cggttgtggc aatgcttgcc gcctgctctt ccaaaccaac cgatcgcgga
    24241 cagcaatata aagacgggaa atttacccag cctttctctc tggtgaacca gccagatgcc
    24301 gttggcgcgc cgattaacgc cggtgatttt gccgagcaaa ttaaccatat ccgtaattcg
    24361 tcaccgcgtc tgtatggcaa ccagagtaat gtttataacg cggtgcaaga gtggctgcgc
    24421 gcaggcggtg atacccgcaa tatgcgccag ttcggcattg atgcctggca gatggaaggt
    24481 gccgacaact atggtaacgt gcagtttaca ggctattaca cgccggtaat tcaggcgcgc
    24541 catacccgcc agggcgagtt ccagtatcct atttaccgta tgccgccaaa acgtggtcgt
    24601 ctgccgtctc gtgcggagat ctacgccggg gcattgggcg ataaatacat cctcgcttac
    24661 agcaactctc tgatggataa cttcattatg gatgtgcagg gtagtgggta tatcgacttt
    24721 ggtgatggca gtccgcttaa ctttttcagc tatgcaggga aaaacggtca tgcctatcgc
    24781 agcattggta aggtgctgat cgaccgtggc gaagtgaaaa aagaagatat gtcgatgcag
    24841 gcgattcgtc attggggcaa aacacacagt gaagccgagg ttcgcgagct gctggaacag
    24901 aacccgtctt tcgtcttctt taaaccgcaa tcttttgcac cggtgaaagg ggcaagtgcg
    24961 gtgccgctgg ttggccgtgc ttctgttgcc tccgatcgtt caattattcc gccaggtact
    25021 accttgctgg cagaagtgcc gctgctggat aataacggca aatttaacgg tcagtacgaa
    25081 ttgcgtctga tggtggcgtt ggatgtcggg ggcgcaatca aaggccaaca cttcgatatc
    25141 tatcaaggga tcgggccgga agccggacac cgcgcaggtt ggtacaacca ctatggacgg
    25201 gtttgggtgc tgaaaacggc tccgggcgca ggtaacgtct ttagcggctg atgtgatatt
    25261 ctgtatgccg tttttacgga tatgacaggg tgaggggtaa cctcacatcc tgattattga
    25321 ctaagtgcgc gttgttcata ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcat
    25381 gcaaatccaa aatattgcag gagttatgta ggcctgataa gcgtagcgca tcaggcattt
    25441 atgcgtttag ggtgagggat aacctcgccc tttttaattc tgaggtgcta tgtctgtggt
    25501 aattagtgat gcatggcgtc agcgttttgg tggcacagcg cgtctgtatg gtgaaaaagc
    25561 gttgcaactg tttgctgacg cgcatatttg tgtggttggt atcggtggtg tcggttcctg
    25621 ggcggcggaa gcgctggcgc gcacggggat tggcgcaatc acgcttatcg atatggatga
    25681 tgtgtgcgtc accaatacca atcggcaaat tcacgctctg cgcgataacg tcgggctggc
    25741 aaaagcggaa gtcatggcgg agcgcattcg ccagattaac ccggagtgcc gtgtaacggt
    25801 ggtggatgat ttcgtgacgc cggataacgt agcgcaatat atgagtgccg gttattcgta
    25861 cgtgattgat gccattgata gtgtacggcc caaagcggcg ctgattgctt attgtcggcg
    25921 caataaaatc ccgctggtca cgaccggtgg cgcgggtggg cagattgacc cgacgcagat
    25981 tcaggttacc gatctggcga aaacgattca ggatccgctg gcggcgaagt tgcgcgagcg
    26041 cctgaaaagc gattttggcg tagtgaaaaa cagtaaaggt aagctcggcg tggattgcgt
    26101 gttttctact gaagcgctgg tgtacccgca gtcagacggt acggtgtgtg cgatgaaagc
    26161 cacggcagaa gggccgaagc ggatggattg tgcatctgga tttggcgcgg caacgatggt
    26221 gaccgccacc tttgggtttg ttgcggtttc tcatgcgctg aagaagatga tggcgaaagc
    26281 ggcgcgtcag ggttaaacaa tcaggtcgga tgagacgcga acgtcttatc cgacccggtt
    26341 ctgtgcttaa acctgcttcg tcgcagcgat aatcgcctcg cttaacgcat ttaacccctg
    26401 gctgcgtgag gcgctaagct gcgcacgtaa tcccagctca tcaaacaatg ccagcggcga
    26461 ctgtgcctgt aattcggcgg cggttttccc ctcaacggca gtcaacaaca ccgccaacag
    26521 gccgcgcaca atgcgccctt cgctgtcgcc aaagaagtgc attttgccgt tttcagccac
    26581 ggtatatccc agccagacgc ggttttcgca tccggcaatc tctttagcct gcgcttttaa
    26641 ctcgtctggc aaagccggaa gctgtttccc cagcatgatc aactggcgat atttatcttc
    26701 ccattgcgac aacggtgcga aggtattgcg taacgtttct gcggttacgg ttgtgccgaa
    26761 cggatgtccg gcgaattgcg ggtttgtcat taatccacca ataattccag cgcgcggtca
    26821 acggcattca ccagcgcatc cacatcactc tttgtattat atggcgcaaa tgaggcgcgc
    26881 agtgtgccgg ttacgcctaa ttctgccagt agcggctgag cgcaatgttg cccggcccgc
    26941 aaggcaatac cgtactccgc cagtagcgtc accatatcgc tgtgatgaac gccagcaaaa
    27001 tcaaaggcca gcaggctgga atcctggcag cggaatgaac gaaagcctgg acgtttcgcc
    27061 agcgcttctt ctgcaagcgt tgctaagcta cggctccagc tttcggcctg gttgatatcg
    27121 taatctgcca gccattccag cgccgcgctt aatcctatga cgccagcgac atttggcgtt
    27181 cctgcttcca gtttccacgg cgcagattga gtggtgaaac cgtcaaaact cacttcgtga
    27241 atcattttgc cgccacccag ccagggcgac atcgcttcca gcagttctga tttgccatac
    27301 agcacgccga tacccgtcgg gccatacagt ttgtgaccag agaaagcata gaaatcgata
    27361 tccagttgct gaacatccgc ggggaaatgc accgccccct gagcaccatc aaccatcacc
    27421 accatcccgg ctgaatgagc aaaggtaatc gctcgcgcca gatccgggca accgccagtc
    27481 acgttcgaca tctgacccaa cgccagaatc cgactacggg gagtaatcag ttctggcaac
    27541 aaatcgacat ccggcagtcg ctgcgcatta agcggcaatt tcaccacttt ggctctagtt
    27601 tgttgggcga ccatcagcca ggggacgagg ttagcgtggt gttctgccac gctgacaata
    27661 atctcatcgc ccggttgcag acgcggacgc gcatagcatt gtgccaccat gttgatggat
    27721 tcagtggtgc cgcgcgtcca gacgatagtt ttatcatccg gtgcattcag taattgcgcc
    27781 actttctctc gtgcagcttc ataacgcgcg gtcaggcgtt gggcttcggc aaactggctg
    27841 cgatggacgt ttccggcgct caggctgtaa aactgtcgag tggcttcaac cacggcttca
    27901 ggtttaagcg cggtcgcggc gctgtcgaga tagacgcccg catcctgtag tgcgggaaac
    27961 tgggcgcgaa actgcgcggg attaaaaacg ttcatggtac tcctcggctt gatgcaccga
    28021 tcgtgacgca aaattcactt tgatacaagg agagtgctaa ccatcagaat tatctagata
    28081 tcctggcgaa gccgcgtaag taacatatgc tgaatagtgt taggcgttaa ttttagcctg
    28141 ttaatcaata ctattaagat ttattaatcg ctttaaggag aagatgatga aaaagactgc
    28201 cgcaattatt tctgcctgta tgctgacttt tgccctgagc gcctgttccg gttcgaacta
    28261 tgtgatgcac accaatgacg gacgtaccat cgtctctgac ggcaaaccac agactgataa
    28321 cgataccggt atgatttcgt ataaagacgc taatggcaac aaacagcaga tcaaccgtac
    28381 tgacgtgaaa gagatggtcg aactggaaca gtaattcgcg atcgcaaggt aaaaaaaagc
    28441 accgcaatta ggcggtgcta cattaatcac tatggacaga cagggtaaat gtacaggaag
    28501 tgaaaaaagg tagctttgct accatggtct gaatcgcaga ccaattgcaa acacaacaac
    28561 acaacatcac aaccgtaagc caaaagttca ccagaatacg cattccgata aaacttttcg
    28621 ttccggctca ggaagtgccg ccactatagg tatttgctgg tagaagctca acggacaatt
    28681 tataatggct cagattaaaa aaactaatag gttacacagt gtgatctaat tgttaaattc
    28741 atttaacatc aaagtttaaa agccatgtct aaacgattac caccgctaaa tgccttacga
    28801 gtttttgatg ccgcagcacg ccatttaagt tttactcgcg cagcagaaga gctttttgtg
    28861 acccaggccg cagtaagtca tcaaatcaag tctcttgagg attttttggg gctaaaactg
    28921 ttccgccgcc gtaatcgttc actcctgctg accgaggaag ggcaaagcta tttcctcgat
    28981 atcaaagaga tattttcgca attaaccgaa gcgacgcgta aactccaggc ccgtagcgcc
    29041 aagggggcgt tgacggtcag tttactcccc agtttcgcca ttcattggtt ggttccgcga
    29101 ctttccagct ttaattcagc ttatccggga attgacgttc gaatccaggc ggttgatcgt
    29161 caggaagata agctggcgga tgatgttgat gtggcgatat tttatggtcg gggcaactgg
    29221 ccggggctac gggtggaaaa actgtacgcc gaatatttat tgccggtgtg ttcgccgcta
    29281 ctgctgacag gcgaaaaacc cttgaagacc ccggaagatc tggctaaaca tacgttatta
    29341 catgatgcgt cacgccgtga ctggcagaca tatacccgac agttggggtt aaatcatatc
    29401 aacgttcagc aagggccaat ttttagtcat agcgccatgg tgctgcaagc ggctattcac
    29461 gggcagggag tggcgctggc aaataacgtg atggcgcaat ctgaaatcga ggccggacgt
    29521 cttgtttgcc cgtttaatga tgttctggtc agtaaaaatg ctttttatct ggtttgtcat
    29581 gacagccagg cagaactggg taaaatagcc gcctttcgcc aatggatcct ggcgaaagcc
    29641 gctgctgaac aagaaaaatt ccgctttcgt tatgaacaat aatttacgta gggtacgacc
    29701 atgaccagcc gttttatgct gattttcgcc gccattagcg gcttcatttt tgtggcactg
    29761 ggcgcttttg gtgcgcatgt gttaagtaaa accatggggg ccgttgagat gggctggatc
    29821 caaaccggtc tcgaatacca ggcgtttcat acgctggcga tcttaggtct ggcggtggca
    29881 atgcagcgtc gcatcagtat ctggttttac tggagtagcg ttttcctcgc gttaggcacg
    29941 gtgctgttca gcggcagcct ttattgcctg gcgctgtccc atctgcgttt gtgggcgttt
    30001 gtcacaccgg ttggcggcgt gagcttcctt gcgggctggg cgttaatgtt agttggtgct
    30061 atccgtttaa agcgcaaggg cgtaagtcat gaataaggtt gtattgctgt gccgtccggg
    30121 ctttgaaaaa gagtgcgccg cagaaattac cgataaagcc ggccagcggg aaattttcgg
    30181 ttttgcccgc gtgaaagaga atgcgggtta tgtcatttat gaatgttatc aacctgatga
    30241 tggcgataag ttaatccgtg agctgccgtt cagttcatta atttttgccc gccagtggtt
    30301 tgtggtgggg gaactcctgc agcatttgcc gccagaagat cgtattaccc ccattgtcgg
    30361 catgttacag ggtgtagtag agaagggcgg tgaactgcgt gttgaagttg ccgataccaa
    30421 cgaaagcaaa gagttgctga aattctgccg taagttcact gttccgctac gcgctgcctt
    30481 gcgcgatgct ggggtgctgg cgaactatga aacgccgaag cgtccggttg tgcatgtatt
    30541 ctttattgca ccaggatgct gctataccgg ttactcatac agcaacaata attcgccgtt
    30601 ctatatgggc attccgcgcc tgaaatttcc ggcagaggcg ccgagtcgtt ccacgctcaa
    30661 actggaagag gcatttcatg tgtttattcc tgcggatgag tgggatgaac gcctggcgaa
    30721 cgggatgtgg gcggtggatt taggcgcttg ccctggcggc tggacctacc aactggtgaa
    30781 gcgcaacatg tgggtttatt ccgtcgacaa cggcccgatg gcgcaaagtc tgatggatac
    30841 cgggcaggtg acgtggctgc gggaagacgg tttcaaattc cgtccgacac gcagcaatat
    30901 ctcctggatg gtatgcgata tggttgaaaa accggctaaa gttgcggcat tgatggcgca
    30961 gtggctggtt aatggctggt gccgtgaaac cattttcaat ctcaaactgc cgatgaaaaa
    31021 acgctacgaa gaagtatcac acaatctggc gtatattcag gcacagcttg atgaacatgg
    31081 cataaatgct cagattcagg cgcggcagtt gtatcacgat cgcgaagaag tgacggtgca
    31141 cgtccgccgc atctgggctg cggtgggtgg tcgtcgcgac gagcgataac aggcaaggcc
    31201 ggggcgtcgt ctccggcctg ctaccctttc cgtcaggctg ttaccaaaga agttgcaacc
    31261 tggcgctcat cagaaataat gtgcgttacc tcatccagat acccgatgcg ggcttcccct
    31321 gaacgattaa atttactttt atcaatcaat aacaacgatt gcgcggcacg ttttaatagc
    31381 atcgatttgt aatcagcgtt gatcgcattg gagtcccaca gtgcgccgct gctatcgatc
    31441 ccttcacaag aaaaaataaa cagatcgatt tccagcgatt taagttggga aatcagcgag
    31501 ggattgacgt aacagccata tttgcgctca agtgtgccgc cggaactgat cagttgaatg
    31561 cgttcgcgtt taccgagttc atggcaaatc ggatggctat tggtgaagac ctgaatgttg
    31621 atgtcaggca actggcgtgc cagataccag caagttgaac tggcgtctaa ggctatcacc
    31681 atcctttctt caatccacgc gagcgcctcg cgcgcgatat ctgctttatg cgcataatgg
    31741 cttttcagcc tgatgtgaaa gagagcgcta ctgtcttgat tttgacggtg gatatattta
    31801 gcgcgtccat gattgcgcag aattttaccc tgcgtctgta attcattgag atcgcgacga
    31861 atggtttctt tactgacctt tagctgttca gagagagctt ccgtggtcag gctggtatgg
    31921 ttcagcagca ggtcgactat cgcttgctgg cgtgccgctt tcatattttt caccccatca
    31981 ttcgataacc gcccgcgtca tgcgggcgta ccggtatgag attacggtgt tacccctttt
    32041 tttaaaagaa tattcccgta cttcgctcgt tcgcctgtga taacgatcgc aaaggctttt
    32101 tgcgcccgtt cataaaacgc aaaacgattg atgcggatga tgtcaggaca cggggcttgt
    32161 agtgaaagcg cattacggta acgtcgttct acttcaggat cgagagtgtc accctctacc
    32221 gccgccatca tcaccagcgg cggggcataa ctgtccagct caaataacgg gataatcgcc
    32281 tggagcaagt cgctcaccaa caggccatca gcgcggatca cctgcggtcc catcgaatgg
    32341 gcgggaaagt gagcatcgga aaaaataatt tcatctccat gtcccatctc tgccagcact
    32401 ttcaatagtt cgggagaaat taacggcgaa attgttttca gcatttcaca cttcctctat
    32461 aaattcaggt tcagtttgcg ggtagaaata acggtactga taatgaatct gtgcacgggc
    32521 ttcttccggg ctgttaaatt cccctacgcc ataccaaccg aacagcgcag ctcctgcgac
    32581 ggtcgtttcg gcatcgtcga gaacttttac cggaatatca agcatattgg ctttaatctg
    32641 attccacaat gtgttgcgac ttcctccacc gactaacaat aattcatagg ctttaaagtg
    32701 cccgattttt tccagcatct gtagattgcg ctgtaattgc gcagttaacc cttccagcgc
    32761 cgcgcgatag aaatgccccc gcgtggtatt aagcgtcact ccttgccagc cagcgttctg
    32821 acacgacaat aaatcacact gcattttcac accatccgcg ccaggtgcga tcagacgagc
    32881 ttcttcaatc aacatttgcc agggtgtttc agccgtccag aacagttttc tcacccattc
    32941 cagcacgccg gatgccagcc attgcatacc tgggttatac aaccatgcct ggctatctag
    33001 ttcgcaggtg gaaccggcgt actgacttaa cagcgaagta tcaacctagg cgctgcgaac
    33061 cattaaaatt tcccatgtac cggaggagag cacgggttca ttttgttcgg caccagcgcc
    33121 aaaaagggcg aactgggtat cgtgacctgc ggaaatcacc ggtatgccaa cgggtaagcc
    33181 gagcattgct gcggcgctgt tctgtagcgt accaatctgt tcacccgctt ccaccagacg
    33241 agggaagagt cggcgtggaa taccggtggc ttgtaaaatt tgcggactga aatcgcgttg
    33301 ctggatatcc agcatctggc tggttccggc tatcgtgata tcagtagtga attcgccggt
    33361 taaacggtgg ttaatcagcg acgaaatgaa gagccaggcg tgcgcgcgtt ccagcagttg
    33421 tggatgattt tctttcaacc acaccaactt atataacgta ttgaaactaa aggctccgac
    33481 gccagaaata gcctgcaacc gctgtgcgga gattaaccgt tcaatattgt ccataaccgc
    33541 tgctgttcgc ggacatttcc agctaatgat cggatagagc agattgcctt gcttatctac
    33601 cagagcgcca tccacaccaa aggtggtgac ggcgataccg cggatgtggc attcagtcag
    33661 ttcactattg atttgccgac agcaatcagc aaagcgttgc aaaatggcag tctaaagacc
    33721 actggtgcca ggtgttgttt tccatcgcga tatcgctggc attaggcgtt gaggcgcggg
    33781 caacaatttt tccctgccga ttaaccgcga tggccctgac attggtcgcg ccacagtcga
    33841 gtaccaggat aacttcttgt ttcataatcg ctcctgcaat atagccggat aacattgttt
    33901 atccggctaa ccactcttgc ttgttcaggc ccggtagccg gagcgaccgg gcatcacatc
    33961 agggtgtaat gtattaacgc ttgtacaacg gaccgtagtt ctggcaagcg cggtaatcct
    34021 ggccttcaat atccatgccg tgcgcagccc aggcagaagg acgatacact ttggtctctt
    34081 caacgttgtg catacatacc gggatacgca gcatggaggc aagggtgata aagtctgcgc
    34141 caacgtggcc gatggtcaga actccgtggt tggcacccca gttcgccatt accgaataca
    34201 catccgtaaa cggcccttta ccagtgaggc gcggtgcaaa ccaggtggtt ggccaggttg
    34261 agttggtgcg tttgttgagg atgtcatgca catccttcgg caattccacg ctccagcctt
    34321 ccgcgatttg cagtaccggt cccaggcctt tgatgatgtt gacacgagtc atggtgaacg
    34381 ggacgccgcc ttcggtaagg aagcgggaag agtaaccgcc gccacggaag tattcgtgga
    34441 tcgccgggca ccattcggta gcggcgaggc aagcgtcagc ctcttgctga gagatttccc
    34501 agtgcggctt catcgtcggg ttaccttcgc tgtcgcgttg tttacaggaa ccgtccagcg
    34561 cagcagaacc tgagttgatc aaatggatga tgccgtgttc tgccagtccg tccagtttat
    34621 gccccgttac acgctcaatt gcttctggtg accagtaggt acgcacatcg gcaaatacct
    34681 gagcggtgcc ggtgagctgg tgacccatta gcattgccac gccgttaaga ctgtcgtttt
    34741 cggtcgccac gacaaagggt tcgcgcacgc cattccagtc aaatgaactg ttgaggatcg
    34801 cttcggcggt gtcaccattg ggatattgat cggtccagtg acgttgcccc tggaagcccg
    34861 cagcgatggc gttgtagcca agtgattctt ccacgcgacc aatatcggcc agtttgctgt
    34921 tgccttgcat catgtcgcgg atacacatcg ccatcagtaa actttcgcgc agaactgcgc
    34981 ggctttgctc ggcattgcgt tgatactgtt tgttattttc atcttcgcca tagcggaagt
    35041 ttttatcagc ccaggccagt gccatttcca gttcggcttc gtcgtaaatc ttctgatcga
    35101 tacggcgacg cagttcggtc atatccaccg cctggacttt cattcccagc caggattcaa
    35161 agaagttgtg atcaacaata gaaccggcga tacccatcga aacgccgccc agcgacagat
    35221 agcttttacc tttcatgctg gcgacggcca aaccggcgcg ggcaaagcgc agcagttttt
    35281 cttcaacatc ggcaggaatc gatgtgtcat cggcatcctg aacgtcatga ccgtaaatgg
    35341 agaatgccgg gatgcctttc tggctgtgag ctgccagagc cgctgccagg taaacagcgc
    35401 cggggcgttc agtgccgtta aagccccaaa tggctttcgg gcgggttgga tccatgtcga
    35461 tggtttcact gccatagcac cagcaaggcg ttaccgtaat ggtgaggcct acattctggc
    35521 tgctgaattt ttcttcgcaa gcagcggctt cagccatacc cgcgatacag gtatcggaaa
    35581 tgacacactc gacggcagct ccgcaggtat ggcgcagttt ctcggtcagc agtgcggccg
    35641 tagctttcgc catattcatt gtttgttctt caagcgactc acgaacaccc atgcgacgac
    35701 cgtcaataac cgggcggata ccaattttcg gtaagctgat ttttttcatt acagattcct
    35761 cactttattc ggaaaatatg ttcagttagt tgccgtttga gaacggaaac gggcaaagat
    35821 aaagatgacc gcgaagcaga gtgcggggat cagttcagca gtggggatgt tgcccgccgc
    35881 gtcactgaca aaacccatga ccggagtgac aataccgccg ccaataatgg tcataacgat
    35941 gaaggacgaa ccgtatttgg tgtcctggcc gagattctta atgcccagcg agaagattgt
    36001 tgggtactga atcgacataa aggcgctgca taaagtcagg gctattaagc ccacatgacc
    36061 gccagcgaag gctgagatca ggcacagtgc catagcgatt aatgcgtagg cggccaggac
    36121 tttgtgtggt gcgaagcgac tgatgagcca ggtaccggtg atacgaccaa taaagaagca
    36181 caccatggtt ccggttaaat agttagcggc aaagcctgca gtcatacctg gaatttcttc
    36241 tacagcgtag cgaatcaaat agctccagca ggccgtttgt gcgccgacat agcagaattg
    36301 cgctaatacc gcccagcgcc agtgacgaat acgcgccaga cgagaaagcg atgcggagaa
    36361 cgatccttgt ttggcgtcac tgtgattatc actctgcaat gccgggaatt tcgtcagcat
    36421 gatcagcagg gcgaccagta acacgatagc cacgatgatc atataaggtg tctgtaccga
    36481 taatactagg ctgtgtttat acgcactcaa ttgctctgga gacattttat cgagaacgtc
    36541 ttgcgattga tgtggcacgt tagacaaaat aagactttgc ccaaagacaa ccgcgataat
    36601 tgcgc
//
LOCUS       DADMGC010000024        36519 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.024, whole genome shotgun sequence.
ACCESSION   DADMGC010000024 DADMGC010000000
VERSION     DADMGC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.024"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(155..1390)
                     /locus_tag="JNZ73_001201"
     CDS             complement(155..1390)
                     /locus_tag="JNZ73_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAY8731552.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1537..1613)
                     /locus_tag="JNZ73_001202"
     tRNA            complement(1537..1613)
                     /locus_tag="JNZ73_001202"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1577..1579),aa:Pro,seq:tgg)
     gene            complement(1656..1742)
                     /locus_tag="JNZ73_001203"
     tRNA            complement(1656..1742)
                     /locus_tag="JNZ73_001203"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1706..1708),aa:Leu,seq:cag)
     gene            complement(1763..1838)
                     /locus_tag="JNZ73_001204"
     tRNA            complement(1763..1838)
                     /locus_tag="JNZ73_001204"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1803..1805),aa:His,seq:gtg)
     gene            complement(1897..1973)
                     /locus_tag="JNZ73_001205"
     tRNA            complement(1897..1973)
                     /locus_tag="JNZ73_001205"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1937..1939),aa:Arg,seq:ccg)
     gene            complement(2076..3461)
                     /locus_tag="JNZ73_001206"
     CDS             complement(2076..3461)
                     /locus_tag="JNZ73_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8731553.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYITMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3652..4392)
                     /gene="rffM"
                     /locus_tag="JNZ73_001207"
     CDS             complement(3652..4392)
                     /gene="rffM"
                     /locus_tag="JNZ73_001207"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HAY8731554.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4395..5747)
                     /gene="wzyE"
                     /locus_tag="JNZ73_001208"
     CDS             complement(4395..5747)
                     /gene="wzyE"
                     /locus_tag="JNZ73_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HAY8731555.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5744..6823)
                     /gene="rffT"
                     /locus_tag="JNZ73_001209"
                     /gene_synonym="wecF"
     CDS             complement(5744..6823)
                     /gene="rffT"
                     /locus_tag="JNZ73_001209"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HAY8731556.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHACEFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6820..8070)
                     /gene="wzxE"
                     /locus_tag="JNZ73_001210"
     CDS             complement(6820..8070)
                     /gene="wzxE"
                     /locus_tag="JNZ73_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HAY8731557.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRFGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTVMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8072..9202)
                     /locus_tag="JNZ73_001211"
     CDS             complement(8072..9202)
                     /locus_tag="JNZ73_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-glucose transaminase"
                     /protein_id="HAY8731558.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9207..9881)
                     /gene="rffC"
                     /locus_tag="JNZ73_001212"
                     /gene_synonym="wecD"
     CDS             complement(9207..9881)
                     /gene="rffC"
                     /locus_tag="JNZ73_001212"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HAY8731559.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9859..10740)
                     /gene="rfbA"
                     /locus_tag="JNZ73_001213"
     CDS             complement(9859..10740)
                     /gene="rfbA"
                     /locus_tag="JNZ73_001213"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY8731560.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFDYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10759..11826)
                     /gene="rffG"
                     /locus_tag="JNZ73_001214"
     CDS             complement(10759..11826)
                     /gene="rffG"
                     /locus_tag="JNZ73_001214"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY8731561.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11826..13088)
                     /gene="wecC"
                     /locus_tag="JNZ73_001215"
     CDS             complement(11826..13088)
                     /gene="wecC"
                     /locus_tag="JNZ73_001215"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HAY8731562.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13085..14215)
                     /gene="wecB"
                     /locus_tag="JNZ73_001216"
     CDS             complement(13085..14215)
                     /gene="wecB"
                     /locus_tag="JNZ73_001216"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAY8731563.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAVNYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14271..15317)
                     /gene="wzzE"
                     /locus_tag="JNZ73_001217"
     CDS             complement(14271..15317)
                     /gene="wzzE"
                     /locus_tag="JNZ73_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HAY8731564.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15329..16432)
                     /gene="wecA"
                     /locus_tag="JNZ73_001218"
     CDS             complement(15329..16432)
                     /gene="wecA"
                     /locus_tag="JNZ73_001218"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HAY8731565.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16672..17931)
                     /gene="rho"
                     /locus_tag="JNZ73_001219"
     CDS             complement(16672..17931)
                     /gene="rho"
                     /locus_tag="JNZ73_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAY8731566.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18016..18117)
                     /gene="rhoL"
                     /locus_tag="JNZ73_001220"
     CDS             complement(18016..18117)
                     /gene="rhoL"
                     /locus_tag="JNZ73_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HAY8731567.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18258..18587)
                     /gene="trxA"
                     /locus_tag="JNZ73_001221"
     CDS             complement(18258..18587)
                     /gene="trxA"
                     /locus_tag="JNZ73_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HAY8731568.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18718..19983
                     /gene="rhlB"
                     /locus_tag="JNZ73_001222"
     CDS             18718..19983
                     /gene="rhlB"
                     /locus_tag="JNZ73_001222"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HAY8731569.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20117..21601
                     /gene="gppA"
                     /locus_tag="JNZ73_001223"
     CDS             20117..21601
                     /gene="gppA"
                     /locus_tag="JNZ73_001223"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HAY8731570.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21648..23669)
                     /gene="rep"
                     /locus_tag="JNZ73_001224"
     CDS             complement(21648..23669)
                     /gene="rep"
                     /locus_tag="JNZ73_001224"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HAY8731571.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAVKRGK"
     gene            23756..24037
                     /gene="ppiC"
                     /locus_tag="JNZ73_001225"
     CDS             23756..24037
                     /gene="ppiC"
                     /locus_tag="JNZ73_001225"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HAY8731572.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24083..25558)
                     /gene="ilvC"
                     /locus_tag="JNZ73_001226"
     CDS             complement(24083..25558)
                     /gene="ilvC"
                     /locus_tag="JNZ73_001226"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAY8731573.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25708..26598
                     /gene="ilvY"
                     /locus_tag="JNZ73_001227"
     CDS             25708..26598
                     /gene="ilvY"
                     /locus_tag="JNZ73_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HAY8731574.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26595..28139)
                     /gene="ilvA"
                     /locus_tag="JNZ73_001228"
     CDS             complement(26595..28139)
                     /gene="ilvA"
                     /locus_tag="JNZ73_001228"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HAY8731575.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDSHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28142..29992)
                     /gene="ilvD"
                     /locus_tag="JNZ73_001229"
     CDS             complement(28142..29992)
                     /gene="ilvD"
                     /locus_tag="JNZ73_001229"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAY8731576.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(30057..30986)
                     /gene="ilvE"
                     /locus_tag="JNZ73_001230"
     CDS             complement(30057..30986)
                     /gene="ilvE"
                     /locus_tag="JNZ73_001230"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HAY8731577.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31006..31269)
                     /gene="ilvM"
                     /locus_tag="JNZ73_001231"
     CDS             complement(31006..31269)
                     /gene="ilvM"
                     /locus_tag="JNZ73_001231"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HAY8731578.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31266..32912)
                     /locus_tag="JNZ73_001232"
     CDS             complement(31266..32912)
                     /locus_tag="JNZ73_001232"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HAY8731579.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAIAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32915..32965)
                     /locus_tag="JNZ73_001233"
     CDS             complement(32915..32965)
                     /locus_tag="JNZ73_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731580.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33051..33149)
                     /gene="ilvL"
                     /locus_tag="JNZ73_001234"
     CDS             complement(33051..33149)
                     /gene="ilvL"
                     /locus_tag="JNZ73_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HAY8731581.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33501..35021
                     /locus_tag="JNZ73_001235"
     CDS             33501..35021
                     /locus_tag="JNZ73_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HAY8731582.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35046..35384)
                     /gene="maoP"
                     /locus_tag="JNZ73_001236"
     CDS             complement(35046..35384)
                     /gene="maoP"
                     /locus_tag="JNZ73_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HAY8731583.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35503..36342
                     /gene="hdfR"
                     /locus_tag="JNZ73_001237"
     CDS             35503..36342
                     /gene="hdfR"
                     /locus_tag="JNZ73_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HAY8731584.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36438..36513)
                     /locus_tag="JNZ73_001238"
     tRNA            complement(36438..36513)
                     /locus_tag="JNZ73_001238"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36478..36480),aa:Trp,seq:cca)
BASE COUNT         8920 a         9993 c         9199 g         8407 t
ORIGIN      
        1 ccaccgcacg caaaggcttt gccggatacg atcacccaaa ccagtatcca cagaacgctg
       61 caatttattg aatttgcacg atcatgtagg ccggataagg cgtttacgcc gcatccggca
      121 atcaaccgca ggcggcttcc aaatggccgc ttttttactt attcaccacc atcaaatgtg
      181 cctgcataat gtcactggcc gggcgaccgt gcgccagcaa atcagccatt gctttaagat
      241 atggcggtag atggcggaaa taacgctgat acccggcaca caaataattc agccccggtt
      301 tgccgctggc atcgagcatg aagcggtgtt tcgggcagcc tccccaacat gcttttaaca
      361 cgttacaact gcgacactgc gccggtaatt gcttaaattt atcttcacca aacacctgct
      421 gttgcgggga atcgatcatt tctgcaattg tttgctgatg catattcccc agccgatatt
      481 gcggataaac atagtgatcg caggcgtaaa cgtcgccgtt gtgctcaaca atcaccgagc
      541 gcccacaggt tggctgatga tggcaaaccg cacccggcgc accgacaaaa ttggcaaacg
      601 cccattcgat attcatcacg aaaatcttgc cgacgtcgcg tttgatccag tggtcaaaaa
      661 tcgccaccag aaattcacca aattcatcgg ggcgcacaga ccattccgtc agttccccct
      721 gaatatcacc aggcgcatgt agtttcagtc catcgcggac agctgtttca tcagccaggc
      781 gctcgaccac cggaataaac tggatgaatt cgactcccgc atcgcacaaa aaatcataaa
      841 cctgtaacgg ttgctgcgcg ctggtgcggt tgacgcagac cagcacgtta tagtcgacat
      901 gatgtttttg caggagcgtc agggcacgca tcaccagttt atgcgtgggt ctgccgcctt
      961 tggtcacgcg atattgattg tggatctcag ccggaccatc cagcgataac ccaacaagaa
     1021 aattattttc tgccagaaat gcacaccatt cgtcatcgag cagcacgccg ttagtctgga
     1081 aactgttact tatcttcctg ccagcaccat atttcgcctg tagcgccacg gcacggcggt
     1141 aaaactccag cccgagtagc gttggttcgc cgccctgcca ggtaaaagcc acttcgtttt
     1201 gcagttcgct ggcggcgata tagtgacgga tatacgcttc cagcgtgtca tcgtccatat
     1261 gcgtgactgg cttttcgcgg taaagggatt gtttttcgag ataaaaacag tagtcacagt
     1321 tcagattaca atcggaaccg ctcggtttcg ccatcacatg aaaagcacgc gttggaacct
     1381 gttgcagcat ggtcgctcct tgcctgaagt catccttcaa tacgttaagg gcgaaatcat
     1441 accgtggggc aatgagttat tacgggcgtt tattgcgaag ggagggtgac gaaatgcaca
     1501 gaaaacaaaa aaccccgccg aagcggggtt caaaattggt cggcgagaga ggattcgaac
     1561 ctccgaccca ctggtcccaa accagttgcg ctaccaagct gcgctactcg ccgaaatact
     1621 gctttttgaa tttttagttc aattctttaa agtcgtggtg cgaggggggg gacttgaacc
     1681 cccacgtccg taaggacact aacacctgaa gctagcgcgt ctaccaattc cgccaccttc
     1741 gcattgtcac aacttctaat aatggggtgg ctaatgggat tcgaacccac gacaactgga
     1801 atcacaatcc agggctctac caactgagct atagccacca ctacaaatct tgttacgcgg
     1861 tattactacc accgcagctc aagcgccggg actaaatggc gcgcccgaca ggattcgaac
     1921 ctgagacctc tgcctccgga gggcagcgct ctatccagct gagctacggg cgcttagcgc
     1981 cgttgcgggg gtggataata cggacttccc acccctctgt ctagtccctt tttaaataaa
     2041 ttgctcgttt ggttacggtt tgtgcgtttt gctgcttatt cctccagttt atgcgcttta
     2101 ccgtggcgat taagcccaaa aactttataa atcgccgtca ccgccagcat aaagatgatg
     2161 ccgacaaaca gcgacatacg cgtatcttca ttaaagtaca tgccgatcaa aacgcaaatc
     2221 aggaatgcca ttgttatgta gttggcccac gggaacagga ttgagcggaa cggatggctg
     2281 gcaatcgccg ctttatgtgc acgccgaaaa cgcagctggc ttatcaatat cacaaaccat
     2341 ggcaccatcc ccggaagcac gctggcactg tagacgtaga caaacacacg ctgcggattg
     2401 ggaatgatgt agttcaggca tgagccaatt agcagaatag caatagatac tgccacaccc
     2461 gcaaccggta cgccgtgacg ggaaactttt gccattgccg ccggtaactg acggtttttc
     2521 gccagtgcgt agagcatacg tccgcaactg tacatgccgc tgttacagcc agagagcgca
     2581 gccgtcagca ccacaaagtt gataatgccc gccgctgcgg taataccgat tttggcaaaa
     2641 gtcagtacga acgggctgcc gttgctgcca atttcattcc acgggaagat ggtgacgata
     2701 acgaaaatcg cgcctacgta gaaaatcagg atccgccaca gcactttgcc tacggcactg
     2761 cgcagcgtca cctgcggatt cttcgcttca ccggcagtaa tgccaatcag ctccacgccc
     2821 tggtaggacg ccaccacaat acacagagcg gtcaggaacc ctttccagcc acccgcaaag
     2881 aaaccgccat gctctgtgag attgctaaaa ccaatcgact gcccgccatt gccaaagcca
     2941 aagaaaatca cgcccaggcc aatgacaatc atcacgataa tcgtggtgac tttgatcatc
     3001 gcgaaccaga actcgatttc gccgtacaac cgcaccgccg ccagattcgc caacgccacc
     3061 agcgccaccg cgatcaatgc gggtatccac tgcgccatct ccggggacca aaactggaca
     3121 taaacgccaa tggcggtgat ttctgagatc cccaccgcca tccacataaa ccagtaagac
     3181 caggcggtga gatagccaaa aaacgggctc atataacgat gcgcataaac ggcgaacgaa
     3241 ccggtaaccg gttcgaggaa caacatttcg cccattgaac gcatgatgaa aaaaacgaac
     3301 agcccggcga tgatataggc caacaatacg gatggcccgg cccatttcag ggtactggcg
     3361 gcccccataa acaggccgac gccaatggtg ccccccaggg cgatgagttc gatatgtcga
     3421 gcttccagcc cacgctgtag ctctggtttg ttatctgcca taaatcctcg tgttgtgttt
     3481 gcatgctttc cggtgttacc ggttatcgtt atgggtacat cgagtgttgc gaatgttttc
     3541 gtaattcagg agaaatggca aataaagcat taaaaatttg aatgctttgt gtaataaaaa
     3601 agcagacagg cgacgaagtg accactccgt cgctttacaa agagaggaaa atcagaggtt
     3661 gccggtgtag tgccagcgta aataacgcag caaacgaagc tgacgcttaa tgcggctcgg
     3721 ctgcgaaagc aggcggtaga gccactccag tcccagcgtt tgccagattt tcggtgcgcg
     3781 ttttacgtga ccggtgaaaa catcgtaagt cccgccaacg cccatataca gcgcatctgg
     3841 atgcaccaga cggcagtcgc gcatgaagat ctcctgcttt ggcgatccca tcgcaacggt
     3901 gacgatttgc gctccgctgg catgaatgcg ttcaaacagc gcctgacgct gctcgggttt
     3961 aaaataacca tcctgactgc caacgatatt cacattccat tggttgcgca gtttagcttc
     4021 agtttgcgcc agcacttcag gtttaccgcc cacaagaaat accggcgtcc cttctttgcc
     4081 tgcgcgcgcc atcagctctt cccagagatc ggcaccggca acgcgggaaa cctgcgcctg
     4141 cgggtacttt ttacgtactg aacgtacaac gctgatgcca tccgcatatt taaattcggc
     4201 agcgttaatt aactccctga cctcggcgtt atcttcaatg gtcagcattt tttcggcatt
     4261 aatggcaacc agcgttccct gcttaagctg cccgtcagca aacagataat cgagggcatg
     4321 ctgcatatca cgccaaccaa tcaactgtaa gccacgcagc gtataggtcg gtgccgtggt
     4381 gttgttattc attgttatcc ttcaacctgc gtccggagcg atgattttgt acgtttatga
     4441 atgagtccgg cgctttcaaa aagccagtac aacagttttg cgatcatcag acatgcgccg
     4501 aagaccacga taaaaaagac tacgcgtgag acaaacgaat ccagcccttc acgtgccagc
     4561 acgatcatat tgaagatcgc cccaaagcag aaactgtgca atatcgcagc cttatagcga
     4621 ttagtctcgc ggttgcccag ctcatacagc cagtcgaacc atttgatgat cagtccaacc
     4681 acaatcgccc cgagtgggat gaacaacgcg ccgcccatca ccaccagtga gcctataagc
     4741 gtaggcgaga tcgccagtcc ggagtggtta ttcagtactt cccaggtaaa gtaattggct
     4801 gagttcagca ccatactcgg gcgccccggc cacagccagg aagggataaa gacatagaaa
     4861 tcgcggacaa ttggagccag gccctggaag tcgatgttgt cgtagttctg caacagcaac
     4921 gccagattct cccacggtga aaaggtgtcg cgagtgagat agagaaacgt atagaacgct
     4981 tcatcgccgc tcacattcat tccatagcgt tttaacgcca gccagaacat gccaacaatc
     5041 cccagcacgc ccgccgccgc cagcatccac aacgaaatcc agccgcgaat aatgccaata
     5101 aacaggaaga tagcgaatgc gatgatgata ttggcgcgag tgccgccgac aatcatataa
     5161 gtcagcaagc caaaggcgac cgtgctgacg aggaaaaaca gccacgcttt gctgtcctgg
     5221 cgcagaaagt agaccaccag catcgccggg atgaaaaagt aaaagaagcg ttttaacgcc
     5281 acgccggaga cttcactgga aaagatctga ctgtaggagt taagccggaa cagcaaaaag
     5341 ccgttgtgca tgaagaagat gccgacgctt accagcgcga tacccatcag gatcacccac
     5401 gtaagattgg tctccacgcg gttcatggta aacagcggac ggcgcggtac atcagcaacg
     5461 cgtttgcgta ggcgggtttt gtaggtgaca tagtaaaccg cgtagaagca gcccgcagaa
     5521 agcaacgcct gcaacaagat ttctggcggc gcgacaccaa catcaaagcg aaataccagc
     5581 acgctggtca gcgggaagcc gaagaaaaag gtgagcaaaa acagcaatga aaagaagaca
     5641 ttgaagttaa agcgcacacg gcgaaactca aaccaggtca gcgtggcaat aaacagcgtg
     5701 cagagcagcc agaccacaaa caggccactg aattgcagca gactcatgcg acctccccgg
     5761 cggcaatcgc caacgcccgc tgccagcctt gtagatagtt agggctaaag aaggcaatgg
     5821 tgtttttatc cgccgacgcc aactggcgct gcgcttcacg cacaatatcc tcgttgagat
     5881 cgtcggtagt aaacagcact ggcaaatgct gttccgtcat atcctgccag aacggatttt
     5941 ccctgttaag cacacaagga atgcccgcct gaatcagtaa gcaaagcgta ccaatgccct
     6001 gctggcgggc aaaaataaag taaccaagat cgcactgacg aagtagcgcc agataggcgt
     6061 caaattccag tttttcgctc aaaatttgca gattttcttc gctgaataac tccaaccccg
     6121 cctgacgaac ttcctcaatg tacgcttcgt tattaggcgg atatcccatc ggcaccacca
     6181 cttttaccgt atcgccaaat tgctgatgaa cggcacgtaa agcggcgaca tgctcattgc
     6241 tgcggtcgcc ggagttcccc accagaatgg tcatttttcc ttcacgttgc cgatcgttcg
     6301 ccatcgtatt gagagaaggg tccatccgcg tcgggaagta cagcagttct ccccgcacct
     6361 ttgggtgcgt tttggcaaaa aagctcaaat cgccgcgggt ggcaaataca cagccgactc
     6421 gcttttgcgc caggcgacgt agtgggtaaa aaagcttata tctcaagcca ctggaaagct
     6481 catacaggtc tgccccccag atatgccaga aaaactggct gggcttaatg ccaccactca
     6541 gcagagccag ccacagtgtg ggattgaact gaccgtggaa aaaaaatcgc tgctgacggt
     6601 tagcttttgc tttcgcgatg accgcttccg ccagcgattt tttcccaggg aaaaattgca
     6661 cagaaagcgc cggacagcta tcacttaagc cgtcgtcctt gccaacaacc ataaactcgc
     6721 acgcgtgctc gctcgtcgcg gccagcgcgt cattgaaaaa ccgcaaaacg gttcggttat
     6781 ggtgagggat atccgatccc agtacgtgaa tcagtacagt catgcccgcc tacgccagag
     6841 taaaaacacg ccacaacaaa gagaaaaata gacgatataa gttgccatat atgcctgcgc
     6901 cgcgcccagt gcaccatgcg cagggattag ccagtgggca aataccatca ataaagtgaa
     6961 ctggctgact tccgccagaa tataaaaccg cagtgacgct ttggcgatca ccagataacc
     7021 aaagacataa gcgcccactt ttaacacatc acccactaac tgccaggcaa agagatcgcg
     7081 cataacggta aatttattcg acaacagcaa ccagatagca aaatcacgca gcaaccagac
     7141 ggtaaaactc gctgccgcca ctgccggtaa gacgaatttc agcgatttaa ccacttcccg
     7201 ggtgatatcg cgcttttccg ttagccgcga caacgtgggc agcaaatata cgctgaacga
     7261 tgccgtaata aattgcaggt aggcatcgga aatactgctc accccttgcc agatccccac
     7321 ttcatcccag ctatactgcg ccgccagcag tttacgcatc atgatgtaag caacaggcaa
     7381 ggtcaccgac gtaatcaacg ccatgagcgt aaatttgctc aactgccctg ccagaccgtt
     7441 atcccagctg ggtttcagat agcttaacgg gatgacacca cgtttaatca acatgatggc
     7501 ggcaggaatt accaccagcg cgggaatcag cgccagaccc agcaacgccc cttcataacc
     7561 gccaaaacgg taactgacgt agtacgcgag aacgccaatc aagctgccga caatcagcga
     7621 taacgcatta cctgcggcat cgcgaaagcc tttcatcagc gccagtaaca ggttgcccca
     7681 ggcgatcccc atttgcacca gcgccactaa acgcaccagc ccctgatagt cggtattacc
     7741 aaacaatccc tggctgattg gcgcagctgc cagcacaaaa accagcgcca tcagcgtaga
     7801 gaaaccaagt accatcgctg atgaagtgcc gaccacgcgg cgcagctgtt gcggattatc
     7861 atggtactgg gcaacgtatt tggttacacc gttaaagatg ccagccccgg caagcacgcc
     7921 gagcacggta atcaactggc ggaaatttgc cgccagccca agccccgccg gaccaaatga
     7981 caccgccagc aacttaccga ccagtaaccc ggcaccaatc ttgaccagtg tactggccgc
     8041 cgtccacaag gacgcttttg ccaacgacat atcaggagaa gtagttcaac aaagtcgcaa
     8101 ttaccgtacg ctgattgacg ggcgacaggt tgtagaacag cggcaggcgc agcaggcgct
     8161 cgctctcttt ggtggtgtag cgatcttcac cgtggaactc accaaagtgt tcccccgcag
     8221 ggcaaccgtg cagcggaatg taatgaaaca ccgccatgat ttccgcttct ttcagaaagt
     8281 taatcaacgc gctccggtca tcaatatccc gcagtttaat gtagaacata tgcgcgttct
     8341 gcacgcagcc atcgggaatc gacggcaact cgatacgccc ggctttcgcc agaggcgcta
     8401 acgtatcgta gtagttttgc cacagcgcca gacgttgctg gttgatacga tccgctgctt
     8461 ccagttgcgc ccacaggtac gcagcttgca gatcggacat caaatagctg gagccaatat
     8521 cgcgccaggt atatttatcg acctgaccac ggaagaactg gctgcggtta gtgccctttt
     8581 cacggatgat ctcggctcgt tcgattaacg ctttatcgtt aatcagcgtc gcgccgcctt
     8641 caccacccgc cgtgtagttt ttggtttcat ggaagctaaa gcagccaata tgaccaatgg
     8701 ttcccagtgc acgccctttg taagtggaca tcacgccctg agcggcatct tccaccacaa
     8761 acaaattatg ctttttcgcc aacgccataa tggtgtccat ttcgcaggcc acacccgcgt
     8821 aatggaccgg cacgataacg cgcgttttgt cggtgatcgc tgcttcaatc aacgtttcgt
     8881 cgatgttcat ggtgtccggg cgaacatcca caaagacgat tttcgcgcca cgcagcacaa
     8941 aggcattggc ggtggagaca aaggtgtagc tcggcatgat cacttcatcg ccaggctgga
     9001 tatcgagcag cagcgccgcc atctccagcg aagcggtgca ggacggcgtc agtaacactt
     9061 tggcgctgcc aaaacgttgc tccagccact gctggcagcg acgggtaaaa ccgccatcgc
     9121 cacacagttt gccgctaccc attgccgact gcatatagtc gagttcggtt cccaccaccg
     9181 gcggtgcgtt aaatggaatc atgtgatcac ctgtataacc agtacgcggt gctttctaca
     9241 ttcgcaccac tttgtatgta tcgtttaagc gcggcggtgt tgcccatttg ggtcgccacc
     9301 cgcaaagttg ttttaccgcg agcatacgcc cagtttagcg ccgtttgcat cagctcagca
     9361 cctgcaccgc gtccagccag caggccaatt cgcgcatctg tcgcattgag ttcccgtaaa
     9421 gagacatagc cgcgaatatc gccggacgcc gcacgtagaa tcagacattg atgatcaaag
     9481 gtgccgcgca cggcattttc aatccactgt gcataaaagc gaccgctggc gtcaggcgca
     9541 taccacggcg cacgaaaacg gctttgcgca aatgcggcgc tagctaactg acgtaatgcg
     9601 ggaatatcgg tctcttgtgc cacgacagca ccgctatcac tgacattgtt cacgggtagc
     9661 gccaaatcaa cttcaccttc taccagggag aatcccagct gttgcagggc atccagttcc
     9721 cccgtatttg atgccgcaat tttggcctgc acccgtgacc atggcgctaa ggcgtctgac
     9781 gtcaggagcg gtgcttcaga cgtaatgcgc acgatggcgc tgttaacacc aaagaaggcg
     9841 ttttcccagg ttagtggctc aatactggcg cggacgggca cgaagtaact ccagcagata
     9901 ttggccgtag ccagttttcg ctaatgaact ggcagcacgc ttcacaccct cgtcatcgag
     9961 ccagccgtta cgccaggcga tctcttccag gcaggcaatc ttaaagccct ggcgtttttc
    10021 tactgtctgc acaaaagtgc tggcctcaat caggctgtcg tgagtgccgg tatccagcca
    10081 ggcaaatccg cgcccgagca gttcaacggt caggttgccc gcctcgaggt acatctggtt
    10141 gatggaggta atctccagtt caccgcgctc cgacggcttc acctgctttg cgtactccac
    10201 gactttactg tcgtagaaat aaagcccggt caccgcccag tttgacttcg gctgttttgg
    10261 cttttcttca agcgagatag cgcggaaatt gtcgtcaaat tccaccacgc caaagcgttc
    10321 cgggtccatc acctgatagt caaaaaccgt cgccccttcc gtgcgcgccg caacatgacg
    10381 cagcttcgga ctgaagccct gaccgaagaa gatgttatcg cccagcacca gacaagaagg
    10441 ttcaccatta aggaaggttt caccgatgat aaaggcctgc gccagaccgt ccgggctggg
    10501 ctgttcggca tattccagct ggataccgaa ctcactacca tcgcccagca ggcgctggaa
    10561 ataaccttta tcttccggcg tagtgatgat gagaatttcg cggataccgg ccagcatcag
    10621 caccgacagc ggatagtaaa tcattggctt atcgtaaatc ggcaacagtt gcttcgatac
    10681 gccgcgcgta atcggatgca atcgggtacc ggaaccgccc gccaggataa tacccttcat
    10741 gtttgcctcc gctgaaaatt agcctttcag acctaaacgc tcaccctgat agctgccgtc
    10801 ctgcacctgc ttccaccagc tttcattagc cagataccac tgaaccgttt tacgcattcc
    10861 actttcaaag gtttcctgcg gcagccagcc aagttcacgg gcaattttcg aagcatcaat
    10921 ggcataacgc agatcatgcc ccggacggtc agcgacaaag gtgatcaggt cacgatagtg
    10981 cgccacgccg tgcggcttgt tcgaagccag ttcttccagc agctcgcaaa tggtttccac
    11041 aacatcgaga ttcttacgct cgttgtgacc accaatatta taggtttcac cgactttccc
    11101 ggtggtcgcc acgcaataca gcgcgcgggc gtgatcttcc acatacagcc agtcacggat
    11161 ttgctgcccg ttgccatata ccggcagcgg tttacccgcc agcgcgttga ggatcatcag
    11221 cgggatcagt ttttccggaa agtggtaagg accgtagtta ttcgagcagt tggtgatcag
    11281 cgtcggcaga ccgtaggtcc gcagccaggc gcgcaccaga tggtcgctgc tggcttttga
    11341 cgcggaataa gggctgctcg gcgcatacgg cgtggtttcg gtgaagaaat catccgtcga
    11401 gtgcaggtca ccatatactt cgtcggtgga gatatgatga aaacggaacg ctgatttttt
    11461 atcttccgtc agcgtattcc agtaagcccg cgccgcttca agcaatgtat aagtcccgac
    11521 aatgttggtt tcaataaacg ctgccgggcc gtcaatagaa cggtcaacat ggctttcggc
    11581 tgccagatgc atgacacagt ctggctgatg ctcagtgaat acgcgtgcca gttctgcccg
    11641 atcgcagata tcaactttct caaaggcaaa gcgctcgctt tgcgcgaccg gtgccagcga
    11701 catcaggttt ccggcgtagg tcagcttatc gaccactacc accgcatcgc tcgtttcgtt
    11761 gatgatataa cgcaccagcg ccgagccaat aaacccggca ccacctgtta tcagaatttt
    11821 tctcatcagc gccagactcc tttggcatcg acgacatact gctgatggac attgtcgcca
    11881 ttgataactt tgaactgact atgatcgacc agcatcacca gcacgtctgc cgttgccagc
    11941 gcctcgtcaa gctgcgccag agtacaaagc ccggtcagtt ttttcggcaa ctggtggatg
    12001 ttaggctcaa caaccagcgt ttcgccgctg tgccactggg cgatcagttc agcgatttcc
    12061 atcgccgggc tttcgcgcag gtcatcaata ttcggtttaa acgccagacc aaagcaggcg
    12121 attttcagtt cactggcgcg tttatcggta gcagcaaggc agtcggcaac cgccgctttc
    12181 acctgatcga taacccagaa cggtttgtga tcgttaactt cgcgcgcggt acggataagc
    12241 cgcgcctgct gggggttctg tgccacgata aaccacggat caacagcaat gcagtgaccg
    12301 cccacgccag ggccaggctg aagaatatta acgcgagggt gacgattcgc caggcgaatc
    12361 agttcccaga cgttaatccc ctgatcggca caaatcagcg acaattcatt agcaaaagcg
    12421 atattcacat cgcggaagct gttttcggtg agcttacaca tttccgccgt ccgcgagtta
    12481 gtgacgacac actcaccttc gaggaaaatt ttgtacaatt cgctggcgcg ggccgaacaa
    12541 accggcgtca taccaccaat cacgcgatcg tttttaatca gctcgaccat aacctggccc
    12601 ggtaatacac gttccgggca gtaagcaatg ttgacgtccg cctgctcgcc cacctgctgt
    12661 gggaaagtca gatccgggcg catctctgct aaccattcag ccatcttctc ggttgacccc
    12721 accggcgagg tggattcaag gatcaccagc gcgccttttt tcagcactgg cgcaatggag
    12781 cgagcagccg attcaacgta ggtcatatct ggctcatgat cgcctttaaa cggcgtaggt
    12841 acagcaatca gccaggcatc cgcttcaact ggcgtcgtgc tcgctcgtaa aaaaccgcct
    12901 tctacggcag tttttactac gctcgccaaa tcaggctcga cgatatggat ttcgccacga
    12961 ttgatggtat caaccgcgtg ttggttgata tcgacaccaa tcacctgttt ttgccgtgag
    13021 gcaaacgctg ctgctgtagg cagcccgatg taacccagtc cgataacaga aatggtcgca
    13081 aaactcatag tgatatccga ttatttttta acgcttccag aatgcgagag catgcctgac
    13141 catcaccata cgggttatgg gcgcggctca tagcttgata ttcgttttcg tcttttaaaa
    13201 gacgcgtcac ttcctcgaca attcgctgct tatccgtgcc taccagacgc accgtacccg
    13261 ccgtcaccgc ttccggacgc tcagtggtat cgcgcatcac cagcaccggt ttccccagcg
    13321 aaggcgcttc ttcctgaatg ccgcctgagt cagtcaaaat cagccaggcg tggttcatca
    13381 gccagacaaa cggtaaatac tcctggggat cgatcagaat gacatttttc acatgcccca
    13441 gaatgcgatt gaccggctct ctgacgttcg ggttgagatg caccggatag acaatctgga
    13501 tgtcctggtg cgtggtggcg atgtctgcca gcgcgtggca gatttcttca aagccacgac
    13561 cgaaactctc acgcctatga ccggtcacca gaatcatctt tttatcgggg gcgataaacg
    13621 ggtaatttac cgccagttct gaacgcagcg tgtcgctgct catcacctgg tcacgcaccc
    13681 ataacagtgc atcaatgact gtattaccgg taatgaagat tcggctatcc gcaacgtttt
    13741 cacgcagcaa gttttgccgg gaagtttcgg ttggagagaa gtgatacatc gccagatgcc
    13801 cggtcaatgt acggttagcc tcttccggcc acggcgaata gagatcgccc gtgcgcagac
    13861 cagcctcaac gtgaccaaca ggaatacgct gataaaacgc cgccaggctg gttgccagcg
    13921 tcgtcgtcgt atcgccgtga accagcacga cgtctggttt gaactcggca agaataggtt
    13981 ttagcccttc cagaatccga caggttatct ctgtcaggcc ctgtcctggc tgcattatgt
    14041 tgagatcgta gtcaggtaca atggaaaaga gtttcagtac ctgatcaagc atctctcgat
    14101 gctgcgcagt gacgcaaact ttagcctcaa aaaaaggatc ttttgccaac gcatgcacca
    14161 acggagccat cttgatggct tccgggcgcg taccaaatac agtcagtact ttcacatcga
    14221 ttctcttcga ataagcggcg agcgcctttg cgctcaccgc agcagtgttg ctatttcgag
    14281 caacggcggg ttaatgcgac accagccccg atcagccccc cgacaatgcc ccacataatc
    14341 atcaggaagg cacgacgtgg gctatcgcgt tttaccggtt cttccggcgt acgcaaatag
    14401 cgataggtct gaaaacgcgg atccagggtt ggaccaacat tcagggtgtt taacatggct
    14461 cgattctgat catagtcgag atcaaaggcc ggaccgacgg cctgtaaatt ttccagtcga
    14521 gcctggagca ttggacgccc aagcaggaac atttctgaat caggtaattc ctcggcaggc
    14581 acatctgtcg cactgcgcga aatattatgc tgctcagcaa ttttcagcgc ctgctcaatg
    14641 ctgttcatcc ggcggtcgta gatggctttc gccacctctt cctgacgctt cacctgagct
    14701 ttcatctgga tggtgcgtgc cgcccatgcg cctttcagct catcattcag atggctggct
    14761 gcacgctggc tggcaaaagc aacatactga cgtaacaggt tattagcgtc aggcgcggtt
    14821 tcggcaataa gcttcacgct gtcattgacc gcgcgagtaa agtctccggg gataaactgg
    14881 atgttgttaa tcatttcatc cagcaacgcc gcatcggctt tgctgttgcc caccatccgc
    14941 tgtttgtaat agtcggtttg cagccagaac tctctgcggg tatcccacga ggccagttgc
    15001 ataacaaact ctttataggc ttcgtccatg accgatggtt gatcggcaga agccatgttt
    15061 gaacggacat ccaggttacg caaaaattgc tgctgcgagt aatatccccc cagcatattc
    15121 accgttggac gatcggtaat cgccgtcgag ctccactcct gacgagcaaa aaaagtatac
    15181 gccagcgcga ttaacgcaaa cgccagcccc atgccaataa tccatagctt cccagcccac
    15241 aaggtacgaa acaacccacg aatatccagt tcattttcag cgtcttcggc cggtttccca
    15301 ggcattggtt gtgtcatcac atcctcattt atttggttaa attggggctg ccaccacgat
    15361 ttctacgcag tctgcgtttt acgcgcttaa taaagcgagc aactttccag gcacgcttaa
    15421 tgcaatatcc atagaggaag aatgctagca aaaagagcac cagcatgacc cactccggga
    15481 caaaatgaga atattctgcc agcacgccaa tggaagcgag cagtgctgcg gcaagggtaa
    15541 tcagcacaaa cgcctgacgg gaagtaaacc cggcacgcat gatcaaatgg tgaatatgct
    15601 gacggtcagg agagaatggg ctcatgcctt tacgcaggcg acggtacata atcgccacca
    15661 tatccattag cggaatggcg attatccaca aagcggtaac cgggctgatg ggatgggttt
    15721 tgccctgggt cgtttcgagc aggatccaga taacggtaaa accaatcagc gtactgcccg
    15781 catcacccat aaagactttg tagcggcgac ccaggatacc aaggttaagc atgatgtatg
    15841 gcaggatggc ggcgatcatc gcaaagcacc agattgcgag gctggtttgc ccgtcgaacc
    15901 acaaaatcat accgattgct gcaaacgaga cgcaggacaa cccgcccagc aagccatcaa
    15961 tgccatcaac catgttgaac gcattaatgg ccgcccagac ggcaaatagc gtcaggaagt
    16021 aaccaaacgg tccgagcacc atctcccagg agccaaagat ataacccagg ctactgagat
    16081 aaagtttgcc gaacaccatc ataacaatgc caacagcggc ctgtatggtg gcacggattt
    16141 ttacgctgat atcaaaacga tcatccagcg cgccaatgaa aacaagcaca ccggcacaag
    16201 cgagatagag agatgcatgc ggaatatagt aatcgacaat tccgaacgtg aagcaaatcc
    16261 ctgcgtaaac cgaaatcccc ccaacgagag gtatcaatcc ctggtgacgt ttgcggaagt
    16321 ttggtttatc cactaaaccg actttttttg ccaccttacg ggcaaaaaac agaaacagtg
    16381 tcgtgaataa aaaaatactg atgagatcag tactcactgt cagtaaattc acaatgcatg
    16441 ctctcagaga aaattattag cagaagtata accacgaaga cctttattca gaagggaaag
    16501 tccgttccga acccaccagt cctgtgcaaa aattaaccat ttgatatgca aattgcataa
    16561 catgccagca aattccagga attttcctaa catcgccagc gcgacattaa gattacagat
    16621 ataaaagcaa aacgccacgt aaacacgtgg cgtttttggc ataagacaaa tttatgagcg
    16681 tttcatcatt tcgaagaaat cgtcattggt cttggtcatg gccagtttat taatgaggaa
    16741 ttccattgca tcgatttcgc ccatcgggtg aatgattttg cgcaggatcc acattttctg
    16801 cagttcttcc tgagtcgtga gcagctcttc tttacgggta ccggaacggt tgtagtcgat
    16861 agccgggaag acgcgttttt cagcgatctt acgagagagg tgcagttcca tgttgcctgt
    16921 acctttaaac tcttcgtaaa taacttcgtc cattttagaa ccggtatcga taagcgccgt
    16981 cgcgataatg gtcaggctgc cgccctcttc cacgttacgc gccgcaccaa agaagcgttt
    17041 cggacgatga agggcattgg cgtccacacc accggtcaac actttacctg acgccggaac
    17101 aacagtgttg taagcgcgcg ccagacgagt gatggagtcg agcagaatga taacgtcttt
    17161 cttgtgctca accaggcgtt tggccttctc gatcaccatt tccgcaacct gaacgtggcg
    17221 agatgcgggt tcgtcaaagg tagaagcaac aacttcacct tttaccagac gctgcatctc
    17281 ggttacttct tccggacgtt cgtcgatcag cagaaccatc agcacacaat ccgggtggtt
    17341 gtaagcaatg ctctgagcaa tgttctgcag cagcatagtt ttaccggctt tcggcggtgc
    17401 cacaatcaga ccacgctgac cacgaccgat aggtgatgcc agatccagta cgcgagcagt
    17461 taaatcttca gtagaaccgt taccacgttc catacgcaga cgagagtttg cgtgcagcgg
    17521 ggttaagttc tcaaagagga ttttgttgcg ggcgttttca ggtttgtcga agttaacttc
    17581 gttaactttc agcagcgcaa aatagcgttc accttctttc ggcgggcgaa tcttaccaga
    17641 gatggtatca ccagtgcgga ggttgaaacg gcggatttgg ctaggggaaa cgtagatgtc
    17701 atcaggaccg gcgaggtagg agctgtctgc ggaacggagg aaaccaaatc catcctgcaa
    17761 tatctccagt acgccatcac caaagatatc ttcgccactc tttgcgtgct gcttcaggat
    17821 ggcaaaaata atgtcctgct tacgcatgcg agccaggttt tccagcccca tattttcgcc
    17881 gagagtgatc agctcagaaa ccggcgtatt ctttaattcg gtaagattca tagtggtgtg
    17941 agttcttaaa cttggggtaa atctcgaact taatgttgtg aatggtatgg caggatcatc
    18001 catgcctgtt tacggtcatc gactcatgtc ttttcgctgc ctggtcacag gagagtacgc
    18061 agaactgaaa cgacaagacg gattgagtga cgagccagaa atctgttcac ttcgcattta
    18121 agataaaacg ggaagcgttg gaaattacaa gattcaaact taataaggta tgtttaatac
    18181 gaagtcaaca ctaagttagc atgactcacg ccgggcgtcc agtttttagc gacggggcac
    18241 ccgaacatga aattccctta cgccaggtta gcgtcgagga actctttcaa ctgaccttta
    18301 gacagtgcgc ccactttggt tgccgccact tcaccgtttt tgaacagcag cagagtcggg
    18361 ataccacgga tgccatattt cggcgcagtg ccagggtttt gatcgatgtt cagttttgca
    18421 acggtcagtt tgccctgata ttcgtcagcg atttcatcca gaatcggggc gatcattttg
    18481 cacggaccgc accactctgc ccagaaatcg acgaggatcg ccccgtccgc tttgagtaca
    18541 tccgtgtcaa aactgtcgtc agtcaggtga ataattttat cgctcatata taactccaca
    18601 ggaataagcc tggcgtgttg gtttcgttgt tggtgtaaca ttaaccaact aaaggttgac
    18661 tttatttcac cggatacgct ttcgtaaagc aatagtaagc tgatattcta ccacactatg
    18721 agcaaaacac atttaacaga acagaagttt tccgacttcg ccctgcatcc gaaggttgta
    18781 gaagcccttg aaaaaaaagg gtttcataac tgtacgccca ttcaggcact ggcccttccg
    18841 ctgacgctgg cgggtcgtga cgtagccggg caggcgcaaa ccggtaccgg gaaaacgatg
    18901 gcgtttctta cgtcaacgtt tcattatctt ctctctcatc ctgcgattgc cgatcgcaag
    18961 gtgaatcaac cgcgtgcctt aattatggca ccgacgcgtg aacttgccgt gcagatccac
    19021 gccgacgcgg aaccgctggc ggaagctacc ggcctgaagc tgggcctggc ttacggtggt
    19081 gatggctatg acaaacagct gaaagtgctg gaaagcggcg ttgacattct gattggcacc
    19141 acgggtcgtt taattgacta cgccaagcag aaccacatta acctcggtgc cattcaggta
    19201 gtggtactgg acgaagccga tcgcatgtac gatctaggct ttattaaaga tatccgctgg
    19261 ctgttccgcc gtatgccgcc tgcaaatcag cgcctcaaca tgctgttctc cgccacgctt
    19321 tcgtaccggg tacgtgaact ggcgttcgag cagatgaaca atgccgaata tattgaagtg
    19381 gaaccggaac agaaaacggg ccaccgtatt aaagaagagc ttttctaccc ttctaacgaa
    19441 gaaaaaatgc gtttgctgca aacgctgatc gaagaagagt ggccagacag agcgattatt
    19501 ttcgccaata ccaaacaccg ttgtgaagag atctggggcc acctggcggc agatggtcat
    19561 cgtgtcggtt tgttgacagg cgatgtcgcg cagaaaaaac gtctgcgtat tcttgatgaa
    19621 tttacccgtg gcgatctgga tattctggtt gccaccgacg ttgccgcgcg tggtttgcat
    19681 attccggcag tgacgcacgt ctttaactac gatttacccg atgactgtga agattacgtt
    19741 caccgtattg gtcgtacagg tcgcgcaggc gcaagcggtc actctatcag cctggcgtgt
    19801 gaagagtatg cattgaattt gcctgctatt gagacctata ttggtcactc aattccggta
    19861 agcaaataca atccggacgc attgatgacc gatctgccaa aaccgctgcg cctcacgcgc
    19921 ccgcgcacag gcaatggtcc gcgtcgtact ggcgctccgc gtaatcgtcg tcgttcaggt
    19981 taaataaaat gtcatactca aaatcattca aactgtatca agatggcaag agaatgaatc
    20041 ccgatgagct tactgtagta agtgattcgg gtgaaagaat gcagccaaca cagagacaga
    20101 ttgaaggatg aagagtatgg gttccacctc gtcgctgtat gcagccattg atctcggttc
    20161 gaatagtttt catatgctgg ttgtgcgcga ggtggctgga agcatccaga cgctgacgcg
    20221 aattaaacgc aaagtgcgtc tggctgctgg cctgaatagc gaaaatgacc tctctaatga
    20281 agcaatggag cgcggttggc aatgtctgcg cctgtttgct gaacgtctgc aagatatccc
    20341 tccctcgcaa attcgcgttg tcgctacggc gacattacga cttgccgtca atgcgggtga
    20401 ttttattgcc aaagcgcagg aaatcctcgg ttgtccggta caggtgatca gcggtgaaga
    20461 ggaagcgcgt ctgatttatc agggcgttgc tcacaccact ggtggtgccg atcagcgcct
    20521 ggtggtggat ataggcggtg ccagtactga actggtaacc ggcacgggtg cacaaaccac
    20581 ctcgttgttc agcctgtcga tgggctgcgt cacctggctg gaacgctatt ttgccgatcg
    20641 taatctgggg caagaaaatt ttgatgctgc agaaaaagcg gcacgcgaag tgttacgtcc
    20701 ggttgccgat gaattacggt atcacggctg gaaagtgtgc gttggtgctt ccggcaccgt
    20761 gcaggcgtta caggaaatca tgatggcaca ggggatggat gaacgcatta ccctggaaaa
    20821 gttgcagcaa ttgaaacagc gagccattca ttgcggtcgg ctggaggaac tggagattga
    20881 cgggctgacg ctggaacgtg cgttagtgtt cccgagtggt ctggcgatcc tgatcgccat
    20941 ttttaccgaa ctgaatattc agtgtatgac cctggcgggc ggtgcgctgc gtgaaggcct
    21001 ggtctacggc atgttgcatc tggccgtcga gcaggatatt cgcagccgta cactgcgtaa
    21061 tattcaacgc cgctttatga tcgatattga tcaggcacag cgcgtagcca aagttgcggc
    21121 taacttcttc gatcaggtgg aaaacgaatg gcatcttgaa gcaataagcc gcgatttgct
    21181 catcagcgcc tgtcagcttc atgaaatcgg cctgagcgtt gacttcaaac aagctccgca
    21241 acacgctgct tatctggtgc gtaacctgga tcttcccggt tttacccccg cacagaagaa
    21301 actgctggcg acgctactgc tcaaccagac taacccggtc gatctctcat cgctgcatca
    21361 gcaaaatgcc gtaccaccgc gcgtcgcaga acaactctgc cgtttactcc gcctggctat
    21421 catttttgcc agccgtcgcc gtgacgatct cgtgccagag atgacattac aggctaacca
    21481 tgaactgttg accttgacgc ttccgcaagg ttggctaacc caacatccgc tgggtaaaga
    21541 gattattgct caggaaaacc agtggcagag ctatgtccac tggccgctgg aagtgcatta
    21601 atgagtaagt gccggatgcg atgctgacgc atcttctccg gccttgatta tttccctcgt
    21661 tttaccgcca tcatcgcttt cagattcgcc agatggcttt gccctttctg catccgttct
    21721 tcggcactga ccactttgcg ctcctgttcc caaatcagat catcctgcgg cagctccagc
    21781 aaaaagcggc tcggctccgg gcgcaccagt tcgccgtact gacggcgttc tttacacagc
    21841 gtaaaggtca attccttctg ggcgcgggta atgccgacat aggccagccg ccgctcctca
    21901 tcgatattat cttcatcgat actgctctgg tgcggcaaaa acccttcttc cataccgacc
    21961 atgtagacat aaggaaactc cagccctttt gacgcatgga gagtcatcag ttgcacctga
    22021 tccagctctt cttcactctc accacgctcc atcatgtcgc gtaaagtaaa gcgcgtcacc
    22081 acctgggtga gcgtcatcgg ctcatccagt tcgctgcctt ccagcatctc cgtcatccag
    22141 ctaaacagtt ggttgacgtt cttcatgcgc atttcggcag ctttcgggct gggcgatgtt
    22201 tcgtacagcc aggattcata atccatgccg tggatcagat cgcgcaccgc ggcaatcggc
    22261 tcccgctccg ccagacgctg gatttctgcc aaccagtgag tgaagcgggt caatgcttca
    22321 taaccacgtc cgctaagcgt ctgactcagg cccatatcaa agctggcggt aaacatgctt
    22381 ttattgcgcg tcatcgccca ctcacccagc tttttcagcg tcgccgggcc aatctctcgc
    22441 ttcggcgtgt taacgatacg cagaaatgcg ctgtcatcgt ccgggttagt cagcacgcgc
    22501 agataagcca gcaagtcctt gatttcagga cgagagaaaa acgacgtacc accagaaatt
    22561 ttgtacggga tgcggttttg catcaggaat ttttcaaaca cccgcgactg atggttaccg
    22621 cgataaagaa tagcgtaatc tttgtactgc gttttattga cgaagtgatg cgcaatcagt
    22681 tcaccagtga cgcgctcggc ttcatgttct tcgttattcg cgcttaaaac ttttagctct
    22741 gtgccgtaac ccagttcgga gaacagacgc ttttcaaaga cgtgcgggtt attggcgatc
    22801 aggatgttcg ccgctttcaa aatacgtccg gaagagcggt agttctgctc aagcttaatc
    22861 accttcagcg ccggaaaatc ctggctcagc agcaccaggt tttgcggacg tgcgccgcgc
    22921 caggagtaga tcgactggtc atcgtcaccc accacggtaa agcgcgcgcg gctgcccacc
    22981 agcagtttca ccagttcata ctggctggtg ttggtgtcct gatattcgtc caccagcagg
    23041 tagcgaattt tgttctgcca gcgctcgcgg acttcttcat tgcgttgcag aagtagcgtc
    23101 ggcaataaaa tcagatcatc gaagtcgagc acattacagg ctttcaggtg tgcatcatac
    23161 agcccataac aatgggcaaa aatacggtcc cgctcgccaa tcgcactggc tgccgcctgg
    23221 gacggtgttt tgagatcgtt cttccagtta gagatggtcg aaatcaactg ttgcaggaga
    23281 actttgtcat cttcaatcag cccttcggtc aactctttga gcaacgccag ctgatcggta
    23341 tcgtcaaaca gcgaaaagtt cgccttcatc cccagcgccg catactcacg cttgatgata
    23401 tccagcccca gcgtgtggaa ggttgagatc atcagcccac gcgcctcttt gcgacccagc
    23461 gtctgtccga cacgctcttt catctcgcgc gccgccttat tggtaaaggt caccgccgcg
    23521 atatgtcgcg cctgataacc gcacccgcgg atcagatggg cgattttatt ggtgataaca
    23581 cgagttttac cggaacccgc acccgccagc accaggcagg ggccggtaac gaattcgaca
    23641 gcttgttgtt ggccggggtt tagacgcata ggaaaatcgc tcaatgaaag tcgggagggg
    23701 aaaataacag cgtggtagta tagccagcct catccttacc actcaaggca cgatcatggc
    23761 aaaaacagca gcagcactgc atatccttgt aaaagaagag aaactggctc tggatcttct
    23821 cgtgcagatt aagaacggtg ccgatttcgg caagctggcg aagaaacact ccatttgccc
    23881 atcaggcaaa cgcggcggtg atttaggtga attccgccag ggtcagatgg ttccggcgtt
    23941 cgataaagtt gtgttctctt gtccggtact ggagccaacc ggcccgctgc acacccagtt
    24001 cggttaccac atcattaagg tgctgtaccg taactaatag caaggccttc tccgggagaa
    24061 ggccttgaac tctgaatcgc tattaacccg caacagcaat acgtttcata tccgtcatat
    24121 agccgcgcag tttcttacct acttgctcaa tcgcatggct gcgaatcgct tcatttacat
    24181 cacgcagctg cgcgttatct accgcacctt ccggaatagc tttacccaaa tcgcccggtt
    24241 gcagttctgc cataaacggt ttcagcagcg gcacacaagc gtaagagaac agatagttac
    24301 cgtactcggc agtatcagag ataaccacgt tcatttcata cagacgctta cgggcgatgg
    24361 tgttggcaat cagcggcagt tcgtgcagtg attcatagta agcagattct tcgatgatgc
    24421 cggaatcaac catggtttcg aacgccagtt caacgcccgc tttcaccatc gcgatcatca
    24481 gtacgccttt atcgaagtac tcctgctcgc cgattttgcc ttcatactgc ggcgcggttt
    24541 cgaaggcggt tttgccggtc tcttcacgcc aggtcagcag tttcttatca tcgttggccc
    24601 agtccgccat cataccggag gagaattctc cggagatgat gtcgtccata tgtttctgga
    24661 acagcggtgc catgatctct ttcagctgtt cagacaacgc gtaagcacgc agtttcgccg
    24721 ggttagagag acggtccatc atcagtgtga taccgccctg cttcagcgct tcggtgatgg
    24781 tttcccaacc gaactgaatt agtttttctg cgtatgccgg atcggtgccc tcttccacca
    24841 gcttgtcgaa gcacagcagc gagccagcct gcaacatacc gcacaggatg gtttgctcac
    24901 ccatcaggtc agatttcact tccgcaacga aggacgattc cagcacgccc gcacggtgac
    24961 caccggttgc tgccgcccat gctttagcga tcgccatgcc ttcgcctttc ggatcgtttt
    25021 ccgggtgaac ggcaatcagc gtcggtacgc cgaatccacg tttgtactct tcacgcactt
    25081 cggtgccagg gcatttcggc gcaaccatga ctacggtgat gtctttacgg atctgctcgc
    25141 ccacttcgac gatgttgaaa ccgtgcgagt aacccagcgc cgcgccgtct ttcatcagtg
    25201 gctgtacggt gcgcactaca tcagagtgct gcttgtccgg cgtcaggtta accaccagat
    25261 ccgcctgtgg gatcagttct tcgtaagtac ccactttaaa accattttcg gtcgctttac
    25321 gccaggacgc gcgcttctcg gcaatcgctt ctttacgcag agcgtaggag atatcgagac
    25381 cagaatcacg catgttcaga ccctggttca gaccttgtgc gccacagccg acgatgacta
    25441 cttttttacc ctgaaggtag ctcgcgccat cggcgaattc atcgcgcccc ataaagcgac
    25501 atttgcccag ctgtgccagc tgctgacgca gattcagtgt attgaagtag ttagccatgg
    25561 tgattcctcg tggtgttgtg cttcttattg ttcggttcgc gtttgcgaga ttgaattcac
    25621 tatatgacag gaaatttatt gcggaaattg atatattcac aacgtcacat tgcaatttct
    25681 gcaacgtcaa cattgagggc tgcgccattg gatttacgcg atctgaaaac cttcctgcat
    25741 ctggcggaaa gccgccattt tggccgcagc gcgcgggcga tgcacgttag cccgtccacg
    25801 ctctcacggc agattcagcg cctggaagaa gatctcggcc agccgctgtt tgtgcgcgat
    25861 aaccgcacgg tgacgctgac tgaagcgggc gaagaactgc gcgttttcgc ccagcaaacg
    25921 ctgttgcagt atcagcagct gcgccacacc atcgaccagc aagggccgtt gctctatggc
    25981 gaattacata tcttctgctc ggtgaccgcc gcttacagcc atctgccgcc gattctggat
    26041 cgcttccgcg cggagcaccc gtcggtggag atcaaactca ctaccggtga tgcggcagat
    26101 gcaatggaaa aagtcgtcac aggtgaagct gatctggcta ttgccgggaa gccagagact
    26161 ctgcccggcg cggtggcgtt ttcgatgctg gagaatctgg cagtggtgtt gattgcaccc
    26221 gcattgccct gcccggtgcg taatcaggtt tctgtcgaaa agccggactg gtcaacggta
    26281 ccgtttatca tggccgatca ggggccggtg cgccgccgca ttgagttgtg gttccgacgc
    26341 aataaaatca gtaacccgat gatttacgcc acggttggcg ggcatgaagc gatggtatcg
    26401 atggtggcgc tcggctgtgg cgtggcattg ttgccggaag tggtgctgga aaacagcccc
    26461 gaaccggtgc gtaaccgcgt gatgatttta gagcgcagcg atgagaaaac gccgtttgag
    26521 ctgggcgtgt gtgcgcagaa aaagcggcta catgagccgc taattgaggc attctggaag
    26581 attttgccga accattaacc cgccaaaaag aacctgaacg ccgggttatt ggtttcgtcg
    26641 tggcaatcgt agcccagctc attcagccgg gtttcgaaat ccggttcatg gtcgccaagt
    26701 tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg gaacaaagaa
    26761 atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg tgattccggg
    26821 aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc gaccatatag
    26881 cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta gccgccgtcg
    26941 ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg cacgccgaca
    27001 aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac cgatcgcccg
    27061 ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt caccgccaac
    27121 aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag gccgtggaag
    27181 ttcacgttgg caccggaaag aatatgcgcc agccgttcgc cgcgaatgtt gtgctgggcg
    27241 atatattttt tcattcccgc cagcgccagc gcgccagagg gttccgccac cgcacgcaca
    27301 tcttcgaata aatccttcat cgccgcacag atcgcatcgc tatcgacggt gatgatgtcg
    27361 tcgagatact cctggcataa acggaaggtt tcgtcaccga tgcgttttac cgcgacacct
    27421 tcggcgaaca gtcccacgcg tggcagatca accggatgac ccgcatccag cgctgctttc
    27481 aggcaggcgg agtcttccgc ttctacggcg atcactttga tttgcggcat cagttgtttg
    27541 atcagcaccg ctacgcccgc cgccagaccg ccgccgccga ctggcacaaa tacgcggtcg
    27601 agatgggagt cctgctggag cagttccagc gccagcgtgc cttgcccggc aatcaccatc
    27661 ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc gatcgcttta
    27721 gctttcgctt cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa tccacgtacc
    27781 gcatcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt cacgcctaac
    27841 cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt gatcacgccg
    27901 tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg cagcttaaag
    27961 ctgtgcactg gctggcgatc ttcgcgcttc accagaatga cgttatcaag acgcgacgac
    28021 agtttttcca ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg cgcgcgcagc
    28081 actgctctta aatattcggc accttccgga gcaccggaca ggggttgcga gtcagccatt
    28141 attaaccccc cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg gttgccaggc
    28201 tggcgtaggc gcgcagggca aaggaaacct gacgttcacg gtttttcggc gtccaggctt
    28261 tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg cttacctgta
    28321 actgaatgcc acggttcggg atgtcgatag cgatcaggtc accatcttca atcagaccaa
    28381 tgctgccgcc gcttgccgct tccggtgaga cgtggccgat ggaaagacca gaggtgccgc
    28441 cagagaaacg accgtcggtg atcagcgcac aggctttacc gagacccatt gatttcagga
    28501 agctggttgg gtagagcatt tcctgcatgc cagggccgcc tttcgggcct tcatagcgga
    28561 tcaccaccac atcgcccgcg acaactttgc caccgaggat cgcttctacc gcgtcgtcct
    28621 ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg acgcccgcgg
    28681 ttttcacgat gcagccgttt tcagcaaaat taccgtagag caccgccagg ccgccgtctt
    28741 tgctgtaggc gtgttccagc gagcggatac agccgtttgc gcgatcgtca tcgagagaat
    28801 cccaacggca atcctgcgag aatgcctgtg tagtacgaat gcccgcaggg cctgcgcgga
    28861 acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc agagtttgcg
    28921 gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgcg cgatccagtt
    28981 caccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg tatttttggg
    29041 tgctcggcgc aactttgcat aactgcggga ctttacgcga aagtttgtcg atatcactca
    29101 tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacc gtgttggtag
    29161 agccgcccat cgcgatatcc agcgtcatag cattttcaaa cgccgctttg ctagcaatat
    29221 tgcgcggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat tcaacaatgc
    29281 gtttaccggc attgaggaac aactgcttac ggtccgcgtg ggttgccagc agcgagccgt
    29341 tgcccggctg cgacaggccc agcgcttcgg tcaggcagtt cattgagtta gcggtaaaca
    29401 tcccggagca ggagccgcag gtcggacacg cggaacgttc aacctgatcg ctctgggagt
    29461 cagaaacttt cgggtctgcg ccctggatca tcgcatcaac cagatcgagt ttgattatct
    29521 gatcggacag tttggttttc ccggcctcca tcgggccgcc ggaaacaaag atcaccggaa
    29581 tattcaggcg cagggaagcc atcagcatcc ccggggtgat tttgtcgcag ttagagatgc
    29641 agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca gcgatcagtt
    29701 cgcgagatgg aagtgaataa agcatccccc cgtggcccat ggcaatccca tcatccaccg
    29761 caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg gcgaccagtt
    29821 taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag ttcacaaccg
    29881 cgataatcgg cttgccaaaa tcggcgtcgg tcattccggt ggcgcgccac agcgcacgag
    29941 cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc atactttatt
    30001 tactcccagt gtctgtctcg taaatgggac ggtgcgtgcc gtcccatttt ttgtatttat
    30061 tgattaactt gatctaacca gccccattta tcttcggttt cgccagtgaa gaggccgaag
    30121 aaggcttgct gaatgcgttt ggtaaccggg ccacaacggc cttcgccaat ctgaataccg
    30181 tctacgctgc gcactggcgt gatttctgcc gccgtaccgg acataaagac ttcatccgcc
    30241 aagtacaggg attcgcgcga cagcacctgc tcacgcactt caattcccag ctctttcgcc
    30301 agtttgatga tggcatcacg ggtaataccc ggcagcgcgg aggaggtgaa cggtggggtg
    30361 aacagcacac catctttcac ttcaaacagg ttttcgcctg cgccttcaga gatataaccg
    30421 ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct acccaccagc
    30481 agggaagaga gatagttacc accggctttt gccgcagttg ggatggtgtt tggtgctgcg
    30541 cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc gcccagatac
    30601 gctccccacg ggaaagcggc gataatcaca tcggttgagt atcccgctgg cgggtttact
    30661 cccatgccaa catcaccgac gaagatcagc ggacggatat aggcgctggt gagattgttt
    30721 ttgcggatca cgtcacgaca agcttccatc agctcatcaa tgctctgtga aaccgggaag
    30781 cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg gaatacaacc
    30841 ggtcctttgt gtgagtcgta gcaacggatg ccttcaaaaa ccgaggtgcc atagtgcagc
    30901 gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc attgaaccaa
    30961 atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgttcagg cgcggatttg
    31021 ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca gtttatttaa
    31081 ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga tatttatatt
    31141 ttgtgcatcg ctggcggcag ccatattcat tgagcagacg tggaaaccac gatgacgcac
    31201 cacgcgtaaa acacgttcta aggtttccgg attgaagcga gccgatacat tgacctgatg
    31261 ttgcatcatg ataatttctc caacatttct gaattactgg cacctggcgg caccagcggc
    31321 cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc actgttcagc
    31381 atggtgtcga gtgccgcttc aacctggtct ttacgggtga tgtgttggcc agggatgccg
    31441 aaggcgctgg ctaacatgag gaaatcgggg ttatcggtaa gggtggtttc gctgtatcgt
    31501 tcctgaaaaa acagttgctg ccattgtcga accatcccta accgttggtt atcgagtaag
    31561 acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgcac attcatcatg
    31621 aaagagccgt caccggagat acagacaacg gtatcgttcg gtcgcgcgac ttgtgcgcca
    31681 actgccgccg gtaaaccaaa acccatggta cctaagccgc tggaggtgat gaaattttcc
    31741 gggcgagtgt gggcgatgtg ctgcgcagcc cacatctggt gctgccccac atctgtggtc
    31801 acgacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg agcgtagata
    31861 gcgtcaccgg gatggtcgta acgccaggaa tgttcatcac gcagctgcgc gcagtgttgc
    31921 tgccagtcat tgatatttaa cggctgctgt aatgctggta acagagcatt taaatcacct
    31981 tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat atccatatgg
    32041 ataacactgg cgtgtggtgc gaaggtgttc agtttgccgg tcacccggtc atcaaaacgt
    32101 gcacccacgg cgatcagcag gtcacactcc tgcaccgcga agtttgccgc tttggttccg
    32161 tgcatcccca gcatgcccag atagtacgga taatctgctt ctactgcgcc cagccctttc
    32221 agcgtacagg tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc cggaactgcc
    32281 tgcgccatcc ccacgccacc gccaacgtac agcatcggtt tttgcgcttt tgccagcatc
    32341 tggcgcgctt gctcgacttc ggcatgtggg aaagtcactt cgttttcaac ggtggtgaac
    32401 cacggttcca gatcgccgct ggctaactgg atatcttttg ggatatcgac cagaaccgga
    32461 ccaggacgac ctgagctggc aacgtcgaat gcttcagcca tgatgcgcgg caactcttcc
    32521 agagactgca ccagaaagct gtgcttggta caggctaacg acaatcccag gacatccact
    32581 tcctgaaatg cgtcggtgcc gataaacggt gcggacacct gaccagtgat ggcaacgaca
    32641 gggatggaat ctaacagtgc gtccgcaagc ccggttatca ggttggttgc gcccggacca
    32701 gacgtggcga tacatacgcc agttttaccg gtagcacggg cataaccgat agccgctatt
    32761 gccgcaccct gctcatgtcg gcacagcaag tgctccacgc cgccgtcata caatgcatcg
    32821 taaaccggca taattgcgcc acccggataa ccgaaaacgg tgttcacacc ctgtgcccgc
    32881 aacgcatgta ccacccactg tgcgccattc atagttagtt ccccgtcctg aatcttgaga
    32941 aacagaattt tgtgctgtta ttcattgtct gctcctcggt tatgttttta aggtcaaaaa
    33001 aacccccgga cctttcggtg cgggggtctt agttcgttaa ggcttgatct ctaagccttt
    33061 cctcgtccaa gtgcagcccc gcacggtggg ataataatca ccaccacgct aatcacgacc
    33121 aggctaatca ctcgtagaag ggctgtcatt ttgtcttttc ttgcatcttg ttcgaaggaa
    33181 tgcctaaaga gttaccatag attttgcgaa tagcacaaga tatttttggg cctattttta
    33241 tgatggcttt aataatttca atagaaattg ttgtttttta aaaagaaaat agtgagtgaa
    33301 aaaattcaat ttcagtataa atgttgttcc cttcctcgta gttcatcctt tgtccgctgc
    33361 atttttgcga gcatcattcc accgccagaa caactcaact tcacttgcac aaataaacag
    33421 gaatcagcca ctaaaattca cgaattagcg ggctacagca gcgatatctt atgcacataa
    33481 tggtgactca aggagggttt atgtcactgt caattgttca tacccgcgca gccctggaag
    33541 taaatgcgcc cccaatcact gttgaggtac atatcagtaa aggtctaccc ggcttaacga
    33601 tggtgggctt aacagaaaca acggtgaaag aagctcgcga tcgcgtgcgc agcgccatta
    33661 tcaatagcgg atatgaatat ccggcgaaaa aaatcaccat caaccttgct ccagccgatc
    33721 tgccaaaaga agggggacga tatgatttac ctatcgccat tgcgttgctg gcggcctcag
    33781 aacagcttac agccaataag ttagatgaat atgaattagt cggagaacta gcgcttacag
    33841 gcgctctgcg tggcgttccc ggtgcaatct ccagtgcaac tgaagctatt aagtcgggca
    33901 gaaaaattat cgtcgcgaaa gataacgaag atgaagtggg gctaattaac ggtgaaggat
    33961 gcctgatagc cgatcatctg caggctgtct gtgcgtttct ggaaggtaag aacactctcg
    34021 aacgcccgaa accaactgat gcagtatccc gggcgctaca acatgatctc agtgatgttg
    34081 tcggtcagga gcaaggaaaa cgaggactgg aaattaccgc cgctggtggg cacaaccttt
    34141 tactgattgg gccgccggga acaggtaaaa caatgctcgc cagccgtatt aatggcctgt
    34201 tgccagattt aagcaatgaa gaggcactgg agagcgctgc gatattaagt ctggtaaatg
    34261 ctgaatcagt acaaaaacaa tggcggcagc gcccgttccg ctcacctcat cacagtgcgt
    34321 cgttaactgc gatggtgggc ggtggtgcaa ttccagggcc aggtgaaatt tcgttggcgc
    34381 ataacggcgt gctttttctt gatgagctac ctgaatttga acggcgtaca ctggatgcct
    34441 tgcgagagcc gattgaatcc gggcagatcc atctttcacg cacacgagca aaaataacct
    34501 atccagcccg tttccagctt gttgcggcga tgaatcccag ccctaccgga cattatcagg
    34561 gaaaccataa ccgctgcacg ccagaacaga cattacgtta tctcaaccgg ctctcggggc
    34621 cctttctcga ccgcttcgat ctctcactgg agatcccatt accacccccc ggcattttga
    34681 gtaaaacggt agtgccggga gaaagcagcg ccaccgttaa acaacgcgta atggccgcca
    34741 gagagcgcca atttaagcgg cagaataaac tgaatgcctg gctggatagt ccggaaatac
    34801 gccaattctg caaacttgag agcgaagatg cgatgtggtt ggaagaaacg ctgatccatc
    34861 tggggttatc gattcgtgcc tggcagcggt tactgaaagt tgcacgaacc attgctgata
    34921 ttgatcagtc tgacattatc acacgtcagc atttgcagga ggcagttagc tatcgtgcga
    34981 ttgaccgttt gctcatccat ctgcaaaaac tactgacata aaaaaagggc atttcgccct
    35041 ttttattaat cgtcagaatc ggtgtagtct tcagcacctt caacctgcgg tttaccgccg
    35101 gaaagggtgt gaaaacgttt tggacgctta atacgcgtca tatacttgga ccacacgcgt
    35161 tctgcttctg tcactggctc acgttcgcca cggcatactg ctacgaagag tttctcttcc
    35221 tcggtaaccg gctcgcgttt gccaagatcc aactcattga aggcataacc atgacgctca
    35281 agcagttgtg cctctttgat ggtgaaatca ccatgacgag agaatccacg tggataatgt
    35341 ttattgtcga aatatcgatt agtcgtcgta aagctttccg ccatcctgca cgctcctaat
    35401 tctttgaccg agctagttat ggcgcggagt attagttacg cttgacagag tgtaaaacaa
    35461 aacatttaaa tcataacgac aaataatttt gcggagagca ctgtggatac ggaattgtta
    35521 aaaactttcc tggaagttag ccgaacgcgt cactttggtc gagcggctga atcgctctat
    35581 ctgacccagt cagcagtgag ctttcgaatc agacaactgg aaaatcaact gggtgtgaac
    35641 cttttcaccc gccacagaaa caatatccgt ttaaccgctg ccggtgaaaa actactgcct
    35701 tatgcagaaa cgctcatgag cacctggcag gccgcccgca aggaggtggc gcatacctca
    35761 cgacataacg agttttctat cggtgccagc gcctcgttgt gggaatgtat gcttaatcag
    35821 tggttgggac gcttgtatca aaatcaggat gcccatacag gcttacagtt cgaagcgcga
    35881 attgcccaac ggcagtctct ggtaaaacag ctgcatgaac gccagcttga tcttcttatt
    35941 accactgaag cgcccaaaat ggacgaattt agtagtcagt tgctggggta tttcacttta
    36001 gcgctttata ccagtgcccc ttctaaacta aagggagatc ttaactatct gcgactggag
    36061 tgggggccag attttcaaca gcatgaggca ggtttgatcg gtgctgacga agttcccatt
    36121 ctgacaacca gttctgctga actggcacag caacagattg cgatgcttaa tggttgcacc
    36181 tggctacccg tcagctgggc gcgtaaaaaa ggcggcctgc ataccgttgt cgatagcaca
    36241 acactttcac ggccgcttta tgccatatgg ctgcaaaata gcgataaaaa tgcgttgatt
    36301 cgcgatcttt tgaaaattaa cgtgctggat gaagtgtatt aatctgaatg gctggcaagg
    36361 atgccggtag aaggatttac ttcggagagg attatttcag ataaaaaaaa tccttagctt
    36421 tcgctaagga tgatttctgg caggggcgga gagactcgaa ctcccaacac ccggttttgg
    36481 agaccggtgc tctaccaatt gaactacgcc cctaattag
//
LOCUS       DADMGC010000025        35798 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.025, whole genome shotgun sequence.
ACCESSION   DADMGC010000025 DADMGC010000000
VERSION     DADMGC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.025"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..218)
                     /locus_tag="JNZ73_001239"
     CDS             complement(<1..218)
                     /locus_tag="JNZ73_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY8731585.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAEVY"
     gene            complement(219..305)
                     /locus_tag="JNZ73_001240"
                     /pseudo
     CDS             complement(219..305)
                     /locus_tag="JNZ73_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(399..1874)
                     /locus_tag="JNZ73_001241"
     CDS             complement(399..1874)
                     /locus_tag="JNZ73_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY8731586.1"
                     /translation="MIQSTRISLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEKTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKYFTNKQILPTLILSLALLSGLIYLVETIVVIHKPISDSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTFFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHNI"
     gene            complement(2021..3305)
                     /locus_tag="JNZ73_001242"
                     /pseudo
     CDS             complement(2021..3305)
                     /locus_tag="JNZ73_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
     gene            complement(3418..5352)
                     /gene="yeaG"
                     /locus_tag="JNZ73_001243"
     CDS             complement(3418..5352)
                     /gene="yeaG"
                     /locus_tag="JNZ73_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HAY8731587.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGTLCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEDNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILPRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5788..6534
                     /locus_tag="JNZ73_001244"
     CDS             5788..6534
                     /locus_tag="JNZ73_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HAY8731588.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            6624..7385
                     /locus_tag="JNZ73_001245"
     CDS             6624..7385
                     /locus_tag="JNZ73_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAY8731589.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLVQAGRLRNGLLKNAVVNEIAHV
                     QQNAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(7436..8320)
                     /locus_tag="JNZ73_001246"
     CDS             complement(7436..8320)
                     /locus_tag="JNZ73_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HAY8731590.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGSVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(8404..9399)
                     /gene="gapA"
                     /locus_tag="JNZ73_001247"
     CDS             complement(8404..9399)
                     /gene="gapA"
                     /locus_tag="JNZ73_001247"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY8731591.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9741..10154
                     /gene="msrB"
                     /locus_tag="JNZ73_001248"
     CDS             9741..10154
                     /gene="msrB"
                     /locus_tag="JNZ73_001248"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAY8731592.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            10196..10468
                     /locus_tag="JNZ73_001249"
     CDS             10196..10468
                     /locus_tag="JNZ73_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HAY8731593.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            10838..11914
                     /locus_tag="JNZ73_001250"
     CDS             10838..11914
                     /locus_tag="JNZ73_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY8731594.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            11941..13320
                     /locus_tag="JNZ73_001251"
     CDS             11941..13320
                     /locus_tag="JNZ73_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8731595.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     SLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            13337..14382
                     /locus_tag="JNZ73_001252"
                     /pseudo
     CDS             13337..14382
                     /locus_tag="JNZ73_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            14403..15239
                     /locus_tag="JNZ73_001253"
     CDS             14403..15239
                     /locus_tag="JNZ73_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAY8731596.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            15244..16192
                     /locus_tag="JNZ73_001254"
                     /pseudo
     CDS             15244..16192
                     /locus_tag="JNZ73_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
     gene            16202..17182
                     /gene="ydjG"
                     /locus_tag="JNZ73_001255"
     CDS             16202..17182
                     /gene="ydjG"
                     /locus_tag="JNZ73_001255"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HAY8731597.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            17319..18077
                     /locus_tag="JNZ73_001256"
     CDS             17319..18077
                     /locus_tag="JNZ73_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY8731598.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            18195..19553
                     /locus_tag="JNZ73_001257"
     CDS             18195..19553
                     /locus_tag="JNZ73_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8731599.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFLRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(19646..20287)
                     /gene="pncA"
                     /locus_tag="JNZ73_001258"
     CDS             complement(19646..20287)
                     /gene="pncA"
                     /locus_tag="JNZ73_001258"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HAY8731600.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(20298..21314)
                     /gene="ansA"
                     /locus_tag="JNZ73_001259"
     CDS             complement(20298..21314)
                     /gene="ansA"
                     /locus_tag="JNZ73_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAY8731601.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRL
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNVLAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TLDD"
     gene            complement(21481..23337)
                     /gene="sppA"
                     /locus_tag="JNZ73_001260"
     CDS             complement(21481..23337)
                     /gene="sppA"
                     /locus_tag="JNZ73_001260"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAY8731602.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            23498..24049
                     /locus_tag="JNZ73_001261"
     CDS             23498..24049
                     /locus_tag="JNZ73_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAY8731603.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            24166..25209
                     /gene="selD"
                     /locus_tag="JNZ73_001262"
     CDS             24166..25209
                     /gene="selD"
                     /locus_tag="JNZ73_001262"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAY8731604.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            25214..27175
                     /locus_tag="JNZ73_001263"
     CDS             25214..27175
                     /locus_tag="JNZ73_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAY8731605.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            27302..28342
                     /locus_tag="JNZ73_001264"
     CDS             27302..28342
                     /locus_tag="JNZ73_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY8731606.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQLGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIAVLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(28459..29802)
                     /gene="gdhA"
                     /locus_tag="JNZ73_001265"
     CDS             complement(28459..29802)
                     /gene="gdhA"
                     /locus_tag="JNZ73_001265"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAY8731607.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDTAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            30038..30310
                     /locus_tag="JNZ73_001266"
     CDS             30038..30310
                     /locus_tag="JNZ73_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HAY8731608.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(30276..30683)
                     /gene="nudG"
                     /locus_tag="JNZ73_001267"
     CDS             complement(30276..30683)
                     /gene="nudG"
                     /locus_tag="JNZ73_001267"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HAY8731609.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            30770..31408
                     /locus_tag="JNZ73_001268"
     CDS             30770..31408
                     /locus_tag="JNZ73_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAY8731610.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKSGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(31417..32724)
                     /locus_tag="JNZ73_001269"
     CDS             complement(31417..32724)
                     /locus_tag="JNZ73_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HAY8731611.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPVGGWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPGSSK"
     gene            complement(32791..33444)
                     /locus_tag="JNZ73_001270"
     CDS             complement(32791..33444)
                     /locus_tag="JNZ73_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY8731612.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(33444..34088)
                     /locus_tag="JNZ73_001271"
                     /pseudo
     CDS             complement(33444..34088)
                     /locus_tag="JNZ73_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(34045..34526)
                     /locus_tag="JNZ73_001272"
                     /pseudo
     CDS             complement(34045..34526)
                     /locus_tag="JNZ73_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(34556..34978)
                     /locus_tag="JNZ73_001273"
     CDS             complement(34556..34978)
                     /locus_tag="JNZ73_001273"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731613.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTRWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGSVWASPLQ"
     gene            complement(34951..>35798)
                     /locus_tag="JNZ73_001274"
     CDS             complement(34951..>35798)
                     /locus_tag="JNZ73_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY8731614.1"
                     /translation="KEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGA
                     QLTFIARRDVTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPA
                     ALKEAPDDATFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTF
                     NPAHAQQKIASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDA
                     QLRKADPVVWGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLR
                     RYGTH"
BASE COUNT         9081 a         8478 c         9159 g         9080 t
ORIGIN      
        1 cagtacacct cagcgagagc gcgcatcaca tgcgcggcaa tactctgatt ctgatactta
       61 tcataaagaa aggaggagtc tgctttctgc acgatatagc gcagataggc actcattgct
      121 ctgtcgctgg cgacaagttc aaaaataagg aaggaagatg tcagcacaat gacacttgcg
      181 gcgataaaat gccggagcat tctatggtgc aatttcatga taaatggcac tccttcggtt
      241 agtgaaacga gaaaaccaga taaagttcga ttgcggcctg gcacaggggc gctaattata
      301 ctcatctctg cgccgctggg gaggggaaat ttaggggtaa tagtaataaa ttgtatgcaa
      361 aagccacgca aaaagcatgg ctctgaatat ttttgcactt aaatattatg aataatcact
      421 ttgttgcccc cctcgctttt cgcttcgcgt aaggctttat ctgcttccgt catgacaagc
      481 gagatattac gcgtgtcacc cgttgaaaaa aacgtgccaa tactgatggt catttgttca
      541 ggaacatcat atctgttact gaagccggtc aggagttcga catttttacg catacgttcc
      601 gcaatgattt ttgcctgggc actattgagt tcggtaaata gcaagccaaa cacctcaccc
      661 tccagtcgcg ctaaaatatc atcggggcgg atgctttgct ggataatatc gacaattgaa
      721 accagcactt tatcacccac acgatgtccc cattgggcat tgatgcgttt gaactgatta
      781 atatcaacca gcatgacaca gaagtcctta acaacaggtc gcgacaataa tgactctacg
      841 ctgttgaaaa agtagcgccg attataaata ttggtcagca catcatgaac tgccagtttg
      901 ctggagagtt gcagttcctg aaaaatgtta taaatcagaa aagacacaac aaacagttta
      961 ctggagactt caatcccgcg actgatgtac cagatggtgt aattatactc atccagagtc
     1021 agtagaagta agttgcagca tagcgatgcc agacataata atgctattaa tggccatagt
     1081 tcgctggcta atcgtgtacg catgataata aagaacagta aaaatgccca cagacaaacc
     1141 aatatttttg tatagttgat tccccaggtc gcagtatggt tgtcaggaca gtaatcgacg
     1201 acatacaaag aatagtcagc gttataactg ctcagattgt gtgccagaag gggaaaaact
     1261 aaaaaaggaa tcagcgccag caacagaatg ccagtttttt tcttattgtt gtcaaggatg
     1321 ttgtctttac cataacaaaa gagcgccaac gaggttaaac aaataaaact gagttggcgg
     1381 aaaatataga aaatagaaac atcattcgac tttgtctgga tcagtgtact gtcgctgata
     1441 ggtttatgga taacaacaat agtttcaact aaatagataa ggccacttaa aagtgcaaga
     1501 cttaaaatga gtgttggtag aatttgtttg ttagtgaaat atttcattaa catgaaaaga
     1561 aaaatgattg cgttaaggaa aaacaaagcc acaacgacca tcagataatt catttgtggg
     1621 atgagtttta cattcgtctt ttcgttatat aatgtggtga tgcaaaataa aattaacgtc
     1681 agcaataaac tgaaaataaa aaagctttga gcttttaatt tttgtgtgcc catagaaacc
     1741 ataaaaagga gagcaatgtg agtgctggat tttattgatg gcagagatat atagttttga
     1801 ccaagatcaa ttttcgttaa cgataaaaaa tatttaaaga ataaccccag ggaaatacgt
     1861 gttgactgaa tcatttttcc gaatgattgt tttctggaac taaattgtgt aaaggttatg
     1921 ctgtcatatt taaagcgatt gtaagctaat gtatgtaata aatgagataa tttacagtaa
     1981 gctaaaaatc agccaggcga taatgacccg gctgatagtt ttagtcttta gctgttgcat
     2041 tttgtttatg aaacagttca cggaacaccg gataaatatc atcctggtcg cggatgtgct
     2101 gcatcgcaaa gttgtcgaaa gtagattgca gatgctcata ttctcgcaca caatgtctga
     2161 tgtgcacgac gggtaatttc gatatagctg taataacgaa caacaggtaa taattttttc
     2221 gccaggattt catggcaaag cggagagtca tcggcccagt tatcgccgtc cgatgcttgt
     2281 gcagcgtaaa tattccactg tgccgggtta taacgctctt tcactacctc atccatcagt
     2341 ttcagggcgc tggaaacaat ggtgccgcct gtttcctgcg agtagaaaaa ctcatgttca
     2401 tcgacttctt tcgcctgggt atgatggcgg atgtatacga cttccacgtt cttatacgtt
     2461 ctgctgagga acagatacag cagaatataa aaacgcttag ccatatcttt agtggattga
     2521 tccattgaac cggaaacgtc catcaggcaa aacatcactg cctggctgga gggatcgggc
     2581 cgcttctcgt agttcttgta acgtaaatcg aaggtgtcaa taaaagggac gcgttcaatt
     2641 ttggcacgta attctgcaat ttctttgcgc agacgttcct cttccagcag ctgcgcaggt
     2701 tcactgttgc tgatgatggc caaattctct tccagtgcat gaagttcccg ccgcttgcct
     2761 gccgtcatgg ctgtgcgtcg cgccagtgag ttctgcaagg aacgcacaac gctgatattg
     2821 gctggaacgc cgttagcggt ataacccgcc cgatgcgttt tatattcggt cagctggcgt
     2881 tgttggtttt gtttcagatt cggtaaggcc aaatcttcaa agagcagatc aagatactca
     2941 tctttcgaaa tctgaaagac aaattcatcc tgaccttcac catcctggct ggcctggccc
     3001 tgaccactgc cggaacctcc gccgccaccc tggggacgtt caattcggtc gttctgaacg
     3061 aaatggtcat tgcccggatg cacgcggtgg cgcagaccgc cacgcccctg atgaaacatt
     3121 ggttcgctaa tatcttccgt gggaatggat acggattcgc tgctgtcgac gtcagtcacc
     3181 gaacgcttat taatggcctc ggagatcgac tgtttaattt gcgctttata acggcgtaaa
     3241 aaacgctggc gattcaccat gcttttgttt ttgccgttca gacgccggtc aataaaccag
     3301 gtcatatgcc ccccgtacta catttgccaa ctgtatgcta cgcctgtgtt gccgcttctc
     3361 aggcccacgg ttcgctatcg ttgtaggccc ggtagcatgt gtaccgggcc agggttgtta
     3421 agacgattta cgtacgcgca aataccattc gcacagtaaa cgcacctgtt tacgggtgta
     3481 gcctttttcc atcatacggt cgacaaagtc gtcgtgtttc ttctgctcgt cggttgacgt
     3541 tttggcgtta aacgagataa ccggcaacag ctcctcggta ttggagaaca ttttcttctc
     3601 gataaccgtg cgcagttttt catagctggt ccagttcgga ttgcgtccgc tgttattcgc
     3661 tctggcgcgc agtacgaagt taacaatctc gttgcggaaa tcttttggat tactgatccc
     3721 cgccggtttc tcgattttct ccagctcggc gttaagagac tcgcggtcaa acagctgccc
     3781 ggtatccgga tcgcgatact cctgatcctg aatccagaaa tccgcgtagg taacataacg
     3841 gtcgaaaatg ttttgcccat attcggaata ggattcaagg taggccgtct ggatctcttt
     3901 gccgataaac tcggcatatt tcgggatcag ataacctttc aggaactcca gatagcgttc
     3961 tgcctgctct tgcgggaact gctcgcgctc gatctgctgt tccaggacgt agaacagatg
     4021 gaccgggttt gccgccactt ctacatgatc gaagttgaac acgcggggga ggatcttaaa
     4081 cgcaaaacgc gtcgacagac cgttcatccc ttcatcgaca ccggcgtagt cacgatattc
     4141 ctgatacgac ttggctttcg gatcggtgtc tttcagactt tcgccatcat aaacccgcat
     4201 ctttgaataa atgctggagt tttctggctc tttcaggcgc gaaagaatgg aaaaacgtga
     4261 cagtgtttcg agcgtgccag gggcgcatgg ggcgtgagtc aattcactgt gattaagcaa
     4321 tttctcgtag attttgatct cttcggaaat gcgcaagcaa tacggcacct tcacgatgta
     4381 aacacggtcg aggaaggctt cgttgttttt gttattacgg aaagtgaccc attcggactc
     4441 gttcgagtgg gcgagaataa tcccgttgaa cggcagggcg gagatacctt ccgtcccgtt
     4501 gtagttatct tcctgagtgg cggttaacaa gggatgcagc acttttatcg gtgctttaaa
     4561 catctcaacg aattccatga tcccctgatt ggcgcggcac agcgtaccgg aatagccgta
     4621 ggcgtccggg tcattctgcg cgtagtgttc gagtttacga atatcgactt tcccaaccag
     4681 cgcggagatg tcctggttgt tctcatcacc gggttccgtt ttggcgatag caatttgttg
     4741 cagaattgac ggccagacct tcactacccg gaacttagtg atatcgccac caaattcatg
     4801 caggcgtttt gccgcccatg gcgacatgat ggtgccgaga taacggcgag gaatgccata
     4861 ctctttttcc agaatctgag catcttcctg cggattgaaa agacagaacg gatgatcgtt
     4921 gaccgggcta cgctcaccgt tggcgctcaa tacataaatc ggtacgagct gcattaatga
     4981 tttcagtcgc tcagcaagcg atgatttacc cccacccaca ggccccagca gatacaggat
     5041 ttgtttcttc tcttccagcc cctgagccgc gtgtttcaga taagagacaa tctgttcaat
     5101 cgcgtcttcc atgccgtaaa actcttcaaa tgccggataa cgtgcaatga cccggttaga
     5161 aaagagtcga gaaagtctgg gttcctgggc tgtatcgacc atgacaggct caccgatagc
     5221 catcaatagc cgctcagccg cgttggcata agcactgcga tcttgccgac aagtggtaag
     5281 aaactcctgc agtgtgaact cttcgtcctt ggcagcttca tatcgctggc gatagtgatc
     5341 gaatatattc atggtatgcc gtcctttcgt tttttagcac aggttaagag ccgttcatat
     5401 gaatagtaga ggctcccgga agaggtaaac tgaacgacgt ctcttgctaa aacacagcaa
     5461 ctctcatgcc attttatcga cgatgagaac aacgtgcccg gtgatacacc ttcaaaatta
     5521 agcgtagatg gcaaatggaa aacttgcctg cacggctttc ttaatttcaa tggcatatca
     5581 acaactcatc cataaatttt gcataattaa tgtaaagacc aggctcgcca gtaacgctaa
     5641 atccatttgg ctgtaagcgc ggtgtcatcc gcggcaggaa aattaaacag ttactttaaa
     5701 aaatgaaaac gtaaaaaggt tgggtttcga tgtattgacg ggtaaacttt gtcgcccgct
     5761 aaacatttgt ttaaggaatg attaattgtg accaaactca aacttctggc acttggagtg
     5821 cttatcgcaa cgtctgcagg cgtagcgcac gctgaaggta aattttccct gggcgcaggc
     5881 gtaggtgtcg ttgagcaccc atataaagat tacgataccg atgtttaccc agtaccggta
     5941 atcaactatg aaggcgataa cttctggttc cgtggcttag gtggtggtta ctacctgtgg
     6001 aatgacgcaa cggataaact ttcaattacc gcttactggt cgccgcttta cttcaaagcg
     6061 aaagacagtg gcgatcacca aatgcgtcac ctggatgacc gtaagagcac catgatggct
     6121 ggtctgtctt atgctcactt tacccagtac ggttacctgc gtaccaccct ggctggcgat
     6181 accctggata acagcaacgg cattgtctgg gatatggctt ggttgtatcg ttacaccaac
     6241 ggtggcctga ccgtgactcc gggtattggt gtgcagtgga acagcgaaaa ccagaacgaa
     6301 tactattatg gcgtatcgcg caaagagtcc gctcgcagcg gtctgcgtgg gtataaccca
     6361 aatgacagct ggagccctta ccttgagctg agcgccagct acaacttcct cggcgactgg
     6421 agtgtttacg gtaccgcgcg ctacacccgt ctgtctgatg aagttactga cagcccgatg
     6481 gtggataaat cctggactgg cctgatttct accgggatca cctacaaatt ctgataatgc
     6541 atctttccag ggcattttgt gcataaaaat agggcgtcac gccctgtttt gcattacggt
     6601 ggtgcaatac ctgggaggaa acaatgcaac aaaaaatgat tcaatttagt ggcgatgtct
     6661 cactgccagc catagggcag ggaacatggt atatgggcga agatgccagt cagcgcaaaa
     6721 cagaagttgc tgcactacgc gcaggcattg aactcggttt aaccctcatt gataccgccg
     6781 aaatgtatgc cgatggcggt gccgaaaagg tggttgggga agcattaacc ggtctgcgag
     6841 agaaggtctt tctcgtctct aaagtctatc cgtggaatgc tggcgggcaa aaagcgataa
     6901 atgcatgcga agccagttta cgccgtctca atactgatta tctcgatctc tacttattac
     6961 actggtctgg cagtttcgct tttgaagaga ctgttgctgc gatggaaaaa ttgatcgccc
     7021 agggaaaaat ccgtcgctgg ggcgtttcta accttggtta tgccgatatg caggaactct
     7081 ggcagctgcc ggggggaaat cagtgtgcca ctaatcaggt gctttaccat ctcggttcac
     7141 gagggattga gtacgatcta ctcccctggt gccagcaaca gcagatgccg gtgatggctt
     7201 acagtccatt agtccaggcc gggcggttgc gcaatggact gttaaaaaac gcggtagtca
     7261 acgaaattgc acatgtccaa caaaatgcag ctgcgcttga ggtcgaactt tcgtcagcgg
     7321 aattagctat gctggataag gcatatccgg caccaaaagg aaaaactgcg ctggatatgg
     7381 tgtgatgttg atacgtgccg gatgcatcgc acccggcaca caacattaac gcaaattaac
     7441 gtttggcaac gcgaatggat tgcgccagat gtgcaggttt ctctttggtg actttttgcg
     7501 tttctgaagc gtaagccgtt tctacacaaa caaaggtctt gtagccatcg tccggcatgt
     7561 cgcccatgct aactgaaagc gccggtcccg ggttccagcc gacaacgttc agatgatgct
     7621 ggtggcctac ggcgataata cgattcagcg cttcatcatt aatcacgctg caatcttgcg
     7681 gattcagata cacgcgatcg gtacggtcag ggaaggtctg aataccgtcg gtcagtacat
     7741 cttctttcgc gtcattcact ttatcaatga agcgatcgcc cagcccactg acgcttacct
     7801 tagcgatatc acccacgtta aagtaggtat gcagggcaga ggtggtttca aattcgccat
     7861 gtgattcaag atcgatttca caggttttac ccacgcggaa atgcgctaac agcgtaaagt
     7921 cgtgcggcca gaattttttc gtctcttcgc tttgtgtcaa ttcaaaagtc agcgctacgc
     7981 catcagcatc ttcatgatgt gatttcagcg tccacggcag gttgcgggca aaaccgtgcg
     8041 caggcagacc ttgttgtgcc gccggaccaa accacggcca gcaaaccggt acgctaccgc
     8101 gaatagcgac gccatttttg aacggtgtgt tgttgctcaa ccacagaact tcttcttcac
     8161 ccgcaggttt ccacgagaga aggtgtgcgc cctgtaatgc aaaagaggct tttacctggg
     8221 gatgatcgac cacaatgagg tccagttcat ccagtttacg acgggagagg acaggggaga
     8281 tttgttcgat gaccggaagg gcaaaaattt tcttaatcat gacgcagtcc tttaacttca
     8341 ttttatcagg taaaaaaaag agcgaccgaa gtcgctcttt ttagatcaca gtgtcatctc
     8401 aacttatttg gagatgtgag cgatcaggtc cagaactttg ttggagtaac cggtttcgtt
     8461 gtcgtaccaa gataccagtt tcacgaagtt gtcgttcaga gcgataccag ctttagcatc
     8521 gaacacggaa gtgcaaactt cgccgttgaa atcggtagat actacgtcat cttcggtgta
     8581 gcccagaacg cctttcattt cgccttcagc agcagcttta acggcagctt tgatctgctc
     8641 gtaagttgca gctttttcca gacgaacggt caggtcaact acggatacgt tcggggtcgg
     8701 aacgcggaac gccataccag tcagtttgcc attcagttct ggcagtactt tacctacagc
     8761 tttagcagca ccggtagagg acgggatgat gttctgggaa gcgccgcggc cgccgcgcca
     8821 gtctttgtga gacgggccat caacggtttt ctgagtagcg gtagtagcgt gaacggtggt
     8881 catcagacct tcgatgatgc cgaagttatc gttgataact ttagccagcg gagccaggca
     8941 gttggtggtg caggaagcgt tggaaacgat gtcctggcca gcatatttgt cgaagttagc
     9001 gcctttaacg aacatcggag tgttgtcttt agacggacca gtcataacca ctttcttcgc
     9061 accagcggtg atgtgtttac gagcagtttc gtcagtcagg aacagaccag ttgcttcagc
     9121 gacaacgtca acacccactt cgtcccattt caggttagcc ggatcacgtt cagcggtaac
     9181 acggattttt ttaccgttaa cgatcagatg accgtctttc acttcaacgg taccgtcgaa
     9241 acggccgtga gtggagtcat atttcagcat gtatgccatg taatcagcgt ctaacaggtc
     9301 gttgattgca acgatctcga tgtcagaacg tttctgagca gcacggaaaa caatgcgacc
     9361 gatacggcca aaaccgttga tacctacttt gatagtcata tattccacca gctatttgtt
     9421 agtgaataaa aggttgcctg taaaattaca aaaaccttac gcagcgtcaa gcggaatcgt
     9481 gtcaatcatt gcgacagatc aatcctgtgc ctaagcatta cgcgactgac tcgcctcact
     9541 cttcctttgg gcttgagacc acatggggtc ggcgccccga attttaaagg gcaattacga
     9601 taaaaatgtg attttcatca cgatttaggt gcagccattt cgtcgagatc aagtttagga
     9661 caaaattttg ccagttggtg ttaatgtttt gttagaatca gtcacgtaat gtgagcacga
     9721 ttaaagtgag atgtgagtaa atggcgaata aaccttcggc agaagaactg aaaaaaaatt
     9781 tgtccgagat gcagttttac gtgacgcaga atcatgggac agaaccgcca tttacgggtc
     9841 gtttactgca taacaagcgt gacggcgtat atcactgttt gatctgcgat gccccgctgt
     9901 ttcattccga aaccaagtat gattccggct gtggctggcc cagtttctac gaaccggtga
     9961 gtgaagaatc cattcgttat atcaaagact tgtcacatgg aatgcagcgc atagaaattc
    10021 gttgcggtaa ctgtgatgcc catctggggc atgtctttcc cgacgggccg caaccaacag
    10081 gcgaacgtta ttgtgttaac tctgcttctt tacgcttcac tgatggcgaa aacggcgaag
    10141 aaatcaacgg ttgaaaaaac gattcagcaa cttattccac aggagcggaa aaattatgaa
    10201 tcttgatgac attatcaaca gcatgacgcc tgaggtatac cagcgtttgt cgaccgctgt
    10261 tgaactgggg aaatggcctg atggcgttgc gttgaccgag gaacaaaaag agaattgcct
    10321 gcaactggtg atgctgtggc aagcccgcca taataccgaa gcacagcata tgacaattga
    10381 cacaaatggt cagatggtca tgaagagcaa acagcagtta aaagaagatt ttggtatctc
    10441 agcaaagcct attgccatgt ttaagtaagt ggttcatccc ctttttgccg cggctatgaa
    10501 ggggatagcc aacaatgacc ccacgactcg tctcaattat ctcgacccga cctggctaag
    10561 tcccagtccg atctcttgca gtcgcgttca cgcctgaatg acttctcctg aacttaagtc
    10621 tcagacctat ttggccggta atccctctcg aatttgctct ttttgcgtcc cgccttccac
    10681 caacctgtaa cagacccagc cattttctgc gtaattgtga tctaagttgt aaaatgcagg
    10741 tcattatctg tgttgtgcag atttaatgtt gacctcaaaa atcatgtggg taagtatgat
    10801 ggccatctaa gcaaaacaaa acacagagga tgacgcaatg aaagcactgg ctcggtttgg
    10861 caaggccttt ggcggctaca agatgattca tgtcccacaa cccatttgtg gcccggaaga
    10921 cgtagtgatt gaaattaaag ccgcggcaat ctgcggcgca gacatgaagc actacaatgt
    10981 cgatagcggt tctgatgagt ttaactctat ccgcggccat gagttcgcag gttgtattgc
    11041 gcaggttggt gaaaaagtca aagactggaa agtggggcaa cgcgtcgtgt cggataacag
    11101 cggtcacgtt tgcggcgttt gtccggcctg tgaacagggt gattttctgt gttgtacaga
    11161 aaaagtaaac cttggtctgg ataacaatac ctggggcggt ggtttttcca aatattgtct
    11221 ggttcctggt gaaattctca aaattcatcg tcatgcgttg tgggaaatcc ctgatggtgt
    11281 tgattatgag gacgcagccg tacttgaccc tatctgtaat gcctataaat ccatcgcgca
    11341 gcaatcgaaa ttccttcctg gtcaggatgt tgtcgtcatt ggcactggcc cactcgggct
    11401 gttttccgta caaatggcgc ggattatggg ggcggtaaat atcgtcgtcg ttggtctgca
    11461 agaagatgtg gcggtccgct tcccggttgc aaaagaactg ggggcgacgg cagtagtaaa
    11521 tggttctacc gaagatgtgg tggcgcgctg ccagcaaatt tgtggcaaag ataatctggg
    11581 actggtgatt gaatgctccg gtgccaatat cgcactgaaa caagccatcg aaatgctccg
    11641 cccgaatggg gaagtggtac gcgttggaat gggcttcaaa cctcttgatt tctcgattaa
    11701 tgacattacc gcctggaata aaagcatcat tgggcatatg gcctatgact ccacctcatg
    11761 gcgtaacgct atccggctat tagccagcgg cgctatcaaa gtcaaaccga tgatcacgca
    11821 tcgtatcggc ctgtcgcaat ggcgcgaagg gtttgatgcg atggtcgata aaaccgcaat
    11881 caaagtgatc atgacttacg actttgatga ataactactc tcctgaagga acgttatcgc
    11941 atggaacaga taacaaaacc gcattgtggt gcacggctgg atcgcttacc ggattgccgc
    12001 tggcattcgt caatgtttgc catcgtcgcg tttggcttgc ttgtctgctg gagtaatgcc
    12061 gttggtggct tgatcctcgc gcagctgaaa gcgttgggct ggacagataa ttccaccaca
    12121 gccacattct cagcaatcac gaccgccgga atgtttctcg gtgcgctggt gggcggcatc
    12181 attggcgaca aaaccggtcg cagaaatgcg ttcatcctct atgaggccat tcatattgcc
    12241 tcgatggttg tcggtgcttt ctcaccgaat atggattttc ttattgcctg ccgttttgtg
    12301 atggacgttg ggctgggagc tttactggtt acactgtttg ctggtttcac cgaatatatg
    12361 cccggtagaa atcgcggaac gtggtccagt cgggtttcct ttattggcaa ctggtcatat
    12421 tcgctctgct cactgattgc gatgggactc acgccgctga ttagtgcaga gtggaactgg
    12481 cgggtacaac tgcttattcc tgcaatattg tcgcttatcg ctacggcgct ggcctggcgc
    12541 tactttcctg aatccccgcg ctggctggaa tcgcgcggac ggtatcagga agccgagaaa
    12601 gtcatgcgga gtatagaaga aggcgtcata cgccagacgg gtaaaccttt gccgcctgtg
    12661 gttattgctg atgacggtaa agcgccacaa gcggtgccgt attcagcctt actgacagga
    12721 gtattactga aacgcgtgat attaggctct tgtgtgctga ttaccatgaa cgttgtgcag
    12781 tacacactaa ttaactggtt gccaacaata ttcatgaccc aggggattaa tttaaaagac
    12841 tcgattgttt taaataccat gagtatgttt ggcgcgccat ttggtatttt tattgccatg
    12901 ctggtgatgg ataaaattcc gcgtaaaaca atgggtgtgg ggctattaat cctgattgcg
    12961 gtgctcggat atatctattc actgcaaacc agtatgttgc tcataacgct gattggtttc
    13021 ttcctgatta ctttcgtcta tatgtacgtt tgctatgcct cggcagtgta tgtccctgaa
    13081 atctggccga cagaggccaa actccgtggc tccggtctgg cgaatgcggt agggcgaatc
    13141 agtggtattg ccgcacctta tgccgttgca gtgctgctca gtagttatgg tgtaacggga
    13201 gtctttattc ttctgggggc tgtttcaatt attgtcgcaa ttgctatcgc caccattgga
    13261 attgaaacca aaggtgtctc cgttgaaagt ttaagtattg atgcagtagc caataaataa
    13321 tacgggtgga ttcaaaatga aaaattcaaa agcaatattg caggtgccgg gcacaatgaa
    13381 aattatttca gcagaaatac cagtgcctaa agaagatgaa gttttgatta aagtagaata
    13441 tgtcggtatt tgtggttcag atgtacatgg ttttgaatca ggcccgttta ttccgcctaa
    13501 agacccaaat caagaaattg gcctgggtca tgaatgcgcc gggacggttg tggctgtggg
    13561 aagccgcgtg cgcaaattta aaccggggga tcgggtaaat atcgaacctg gcgttccttg
    13621 cggtcactgt cgttactgtc tggaaggcaa atataacatc tgcccggacg ttgattttat
    13681 ggcgacacaa cccaactacc gcggcgcatt aacgcactat ctgtgtcatc cggagagctt
    13741 tacttacaaa ctgcccgaca atatggacac gatggaaggg gcgctggtgg agcctgccgc
    13801 agtcgggatg catgccgcga tgctggcaga tgttaaaccg ggtaagaaga taattattct
    13861 gggagcaggt tgtattggtt tgatgacgtt gcaagcgtgc aaatgcctgg gcgcaacgga
    13921 aattgccgtc gttgatgtac tggaaaaacg tctggcaatg ggggaacagc ttggtgcgac
    13981 agtggttatt aatggcgcaa aagaggacac tattgcacgc tgtcagcaat ttaccgaaga
    14041 catgggcgca gatattgttt tcgaaacagc gggttctgcg gtcaccgtta aacaggcacc
    14101 tttaatctgg taatgcgcgg cggtaaaatt atgattgttg gtactgtacc cggcgattcg
    14161 gcaatcaatt tcctcaaaat caatcgcgaa gtcactatcc agacggtatt ccgctatgcc
    14221 aatcgttatc cggtcacgat tgaagctatt tcttcagggc gattcgatgt gaaatcgatg
    14281 gtgacgcata tttacgatta tcgggatgta caacaggcat ttgaagagtc agttaacaac
    14341 aagcgcgaca ttattaaagg cgttattaaa attagcgatt aattaatacg ggagaacaga
    14401 acatgctcgc agatatcaga tattgggaaa acgatgccac aaataaacat tatgcaattg
    14461 cccattttaa cgtatggaat gcagaaatgt tgatgggcgt taaagacgca gccgaagaag
    14521 cgaaatcccc ggttattatt tcttttggta caggttttgt cggtaacacc tcatttgaag
    14581 atttctctca catgatggta tcaatggcac aaaaagcaac ggtgccggta ataactcatt
    14641 gggatcatgg tcggagtatg gagattattc ataacgcctg gactcatggt atgaattcat
    14701 taatgcgtga tgcttctgca tttgatttcg aagaaaatat tcgtttaacc aaagaggctg
    14761 ttgatttctt tcatccgctg ggtattccgg tagaggcgga attagggcat gtcggtaatg
    14821 aaaccgttta tgaggaagcg ctggcgggct atcactatac cgatcctgac caggcggctg
    14881 aatttgttga acgtacgggc tgtgactcat tggctgtcgc catcggcaac cagcatgggg
    14941 tttatacgtc agagccgcaa ttgaactttg aggtcgtcaa acgcgtacgc gatgctgttt
    15001 ctgttccgct ggttttgcat ggcgcatcgg ggatcagtga tgccgacatt aaaactgcaa
    15061 tttcgttggg tattgcgaaa atcaacattc atacggagct ctgtcaggcc gcaatggtcg
    15121 cagtgaaaga aaaccaggat cagcctttcc tgcatttaga acgtgaagta cgtaaagctg
    15181 taaaagaacg ggcattggaa aaaattgaac tgttcggttc tgatggcaaa gcggaataat
    15241 gatatggata atctcgacgt tatttgtata ggtgccgcta ttgttgatat tccattacaa
    15301 ccggtcagta aaaatatctt tgatgtagat tcttatcctc ttgaaagaat cgcaatgagc
    15361 accggggggc gatgcaataa atgaagcaac aattatttct cgtctggggc atcgtaccgc
    15421 attaatgagc cgcattggta aagatgcctc agggcaattt attctcggcc attgtcgcaa
    15481 agagaatatt gatattcaaa gcctgaaaca ggacgtcagc atagatacat ctattaacgt
    15541 tggactggtg acggaggatg gcgagcggac gtttgtcacg aaccgtaacg gcagtttgtg
    15601 gaaattaaat attgacgatg ttgattttgc gcggtttcct caggcgaaat tattatcact
    15661 ggccagtatt ttcaacagtc ctctattgga tggtaaagcg ctaacagaaa tttttacgca
    15721 agcgaaagcc cggcagatga tcatttgtgc cgatatgatc aaaccgcggt tgaatgaaac
    15781 gctggatgat atttgcgaag cattgagtta tgtcgattat ctgtttccaa attttgccga
    15841 ggcaaaatta ctcacaggga aagagacact ggatgaaatt gctgactgct ttcttgcgtg
    15901 cggcgtaaaa acggtggtga ttaaaacggg taaagacggc tgctttatca agcgtggtga
    15961 catgacgatg aaggtgccgg cggtcgcagg aataaccgcc atcgacacca ttggcgcggg
    16021 cgataacttt gcttcaggtt ttattgcggc actgttagaa ggcaaaaatc tgcgtgaatg
    16081 cgcacgcttt gccaatgcaa cggcggctat ctcggttcta agcgtcggtg ccaccaccgg
    16141 cgtaaaaaac agaaagctgg tcgaacaatt gctggaagaa tacgaaggat aatgaaggca
    16201 aatgaaaaag atacctttag gcacaacgga tataacgctt tcgcgaatgg ggttggggac
    16261 atgggccatt ggcggcggtc ctgcatggaa tggcgatctc gatcggcaaa tatgtattga
    16321 tacgattctt gaagcccatc gttgtggcat taatctgatt gatactgcgc taggatataa
    16381 ctttggcaat agtgaagtta tcgtcggtca ggcgttaaaa aaactgcccc gtgaacaggt
    16441 tgtagtagaa accaaatgcg gcattgtctg ggaacgaaaa ggaagtttat tcaacaaagt
    16501 tggcgatcgg cagttgtata aaaacctttc cccggaatct atccgcgaag aggtagaagc
    16561 cagcttgcaa cgtctgggta ttgattacat cgatatctat atgacgcact ggcagtcggt
    16621 gccgccattt tttacgccga tagctgaaac tgtcgcagtg cttaatgagt taaaggctga
    16681 agggaaaatt cgcgctatag gcgctgctaa cgtcgatgct gaccatatcc gcgagtatct
    16741 gcaatatggt gaactggata ttattcaggc gaaatacagt attctcgacc gggcaatgga
    16801 aaacgaactg ctgccgctat gtcgtgataa tggcattgtg gttcaggttt attccccgct
    16861 agagcaggga ttgttgaccg gcaccatcac tcgtgattac gttccgggcg gcgctcgggc
    16921 aaataaagtc tggttccagc gtgaaaacat gctgaaagtg attgatatgc ttgaacagtg
    16981 gcagccactt tgtgctcgtt atcagtgcac aattcccact ctggcactgg cgtggatatt
    17041 aaaacagagt gatttaatct ccattcttag tggggctact gcaccggaac aggtacgcga
    17101 aaatgtcgcg gcactgaata tcaacttatc ggatgcagac gcaacattga tgagggaaat
    17161 ggcagaggcc ctggagcgtt aaatactcaa cacgattcac ctctgtttct gtgaaaactg
    17221 tgatgaaaat ctgtgattca aataggttat tttgaaagta aacatcggtt atgcgataat
    17281 cgcgctaatg tgatgtaaaa agatacaggg gtgtaatcgt ggcggcaaaa gacaggattc
    17341 aggcaattaa gcaaatggtt gccaacgata aaaaggtgac agtctcaaat ttgagtggga
    17401 tttttcaggt aaccgaagaa accattcgcc gcgatcttga gaagctggaa gatgaaggct
    17461 ttttgaccag aacctatggt ggtgctgttt taaatacagc ggtgttgacg gagaatatcc
    17521 atttttataa gcgcgcttca tcgttttatg aagagaagca gcttattgca cgcaaggcac
    17581 taccctttat cgacaataaa accaccatgg cagccgattc cagtagtacc gtaatggaat
    17641 tgctcaaatt gttacaggac cgtagtgacc tgacgttgct aaccaactcg gcggaagcga
    17701 ttcatgttct ggctcagtca gaaattaaag tcgtttcaac gggtggggaa ctaaacaaaa
    17761 atacactttc cctgcaagga agaattacta aagagatcat cagccgctac catgtcgata
    17821 tcatggtaat gagttgtaaa ggtcttgata ttaacagcgg cgcgctggac tctaatgaag
    17881 cggaagctga aatcaaaaag acaatgatcc gtcaggcgac agaagttgcg ttattggttg
    17941 atcactctaa gtttgatcgt aaagcttttg tccagttagc tgactttagt catattaatt
    18001 atataataac tgataaatca ccgggtgcag agtggatagc attttgcaaa gacaataata
    18061 tccagctggt gtggtaattt aagcacacgt tgtcaatatt atttgttcct gcgcctggaa
    18121 ataaaatgat gataaagatg cggcaagtga atttgccgca attgctacct tttatagcaa
    18181 atccgaggca cccaatggaa caatatgatc aaattggcgc aagactggac cgcttgcctt
    18241 tggcccggtt tcattatcgt atatttggta taataagctt tagtctgtta ttaacggggt
    18301 ttttgagtta ctcaggtaat gtcgtcttag caaagctggt aagcaatgga tggtcaaata
    18361 atttcctcaa tgccgccttt acctcggcat taatgtttgg ttatttcatc ggctcactta
    18421 ctggtgggtt tattggtgac tactttgggc ggcgcagggc gtttcgcata aatcttctca
    18481 tcgtcggtat tgctgcaaca ggggccgctt ttgtccctga tatgtactgg ctcattttct
    18541 tgcgcttcct gatgggaaca ggaatggggg cgctgattat ggttggctat gcctcattta
    18601 cggagtttat ccccgcgacg gtgcgtggaa aatggtccgc gcggctctca tttgttggta
    18661 actggtcgcc catgctgtct gcggcgatag gcgtggtggt tatcgctttt tttagttggc
    18721 gaataatgtt tcttctgggt ggtattggca tactgttagc ctggtttctc tcaggtaaat
    18781 actttatcga gtcgccacga tggctggcag ggaaagggca aatcgccggt gcagaaagcc
    18841 aacttcgtga agtagagcag caaattgaaa gggagaagag tattcgttta ccccagctta
    18901 ctttgaacca gagcaacagc aaggttaagg taatcaaggg taccttcagg ctcctgttta
    18961 aaggggaaat gttacgacgt acattagttg cgataactgt tttaattgca atgaacattt
    19021 cgctttatac cattaccgta tggataccga ccatatttgt taactccggc attgatgtcg
    19081 ataaatcaat attaatgacc gctgttatta tgattggtgc tccggtagga atatttattg
    19141 cggcattaat tattgatcat tttcctcgtc gattatttgg ctccgcctta cttattatta
    19201 ttgccgtgtt aggctatatc tattcaagtc agactacaga gtgggcgatt ttaatctatg
    19261 gtctggtgat gatcttcttt ttatacatgt atgtttgctt cgcgtcggcg gtttatatcc
    19321 cggagctttg gccaacgcat ttacgcctgc gcggttcggg tttcgttaat gccgtcggac
    19381 ggatcgtcgc agttttcacg ccctatggcg ttgcggcatt attaacacat tatgggtcga
    19441 tcacggtgtt tatggtgctt ggtgtcatgt tagtgctctg tgcgctggtt ctctccattt
    19501 ttggcatcga aacgcggaag gtgtcgctgg aagagatttc tgaggtgaat taaatctttc
    19561 gttgatggca tcaattgcca gatgtaccac tgacgtgttt tatttggcct acaatgccag
    19621 tacagaattg taggccagcg taaaattacc cctgcgtctc ttcccagtct gccagcgtat
    19681 atagcgttgc cccagctgct gacatctcca taaacgcgtg tgcactgtcc tggggctgga
    19741 tattcacgcc acgacagcca tcggtaatca cgtttacctt ataacctaac tgtaacgcgt
    19801 ccagcacggt aaacttcacg caatagtcag tagccaggcc cataacgatc aattcatcga
    19861 tttcatgatc gcgcaaccag tcatcaagcg cggttttctg ccgacgtccg ttatcaaaaa
    19921 aggcactgta actgtcaact aaaggatttt cgcctttatg gaacaccgct gcgatctctt
    19981 tttgattcag taacggatgt aattgtgcgc cttcactgtt ctgcacacag tgttcaggcc
    20041 agaaagtttg ccgcaggccg tcgagttggc ctggcgtata aggctctacg ccgtgctggc
    20101 tggcaaagct gccgtgattc gccggatgcc agtcctggct ggcgataacc gcttctccgc
    20161 gcgactggca ccagtcaata aggcggttag caacctccac cgtactgtca ccttccggta
    20221 cggcgagcgc gccaccagca cagaaatcat tttgtaaatc gaccagtaac agggcgcgat
    20281 ggggcattgc agtctcctta atcatccagc gtcagttcgc cgcgcaggtt ttggctcatg
    20341 gccttgcgaa tggtttcagt atccagttcc tggctcagca ggtaatgcag tttggttagc
    20401 gtggcttcta cagtcatatc tgcaccgcca ataacgccgg catgggcgag gacgttaccg
    20461 gtggcataac cactcatatt cactttaccg gacatacatt gtgtcaggtt gaccaccaca
    20521 ataccgcgat cgctggcttc ttgtaattcc tgcaggaagg ctttgttttg tggcgcatta
    20581 cccacgccat aggagcgcag aatcaatgct ttcaccggtt ggcgcagaaa attgcgcacg
    20641 acgtcagcag aaatccctgg ataaatcgtc actacgccaa ttggttgtgg cgtgattgga
    20701 tgaacgatta attccccttc accgtgcggg gcgggtggcg tattcaaacg acgaatatgg
    20761 ataccagctt ccagtaacgg aggaaggttt ggagaggcaa acgcatcaaa accatcggca
    20821 tgggctttgg tagtgcggtt gccgcgatac aatcggttat tgaaaaagag cgttacttcg
    20881 ttgatcggat aattcgccgc aacgtacaac gcattcagca gattaatttg tccgtcagag
    20941 cgtaactcag ccagcgggat ttgtgaccct gtcacaatga ccggtttacc gagattctcg
    21001 agcatgaacg acagcgcaga ggcggtatac gccatcgtgt cggtaccgtg tagaatgaca
    21061 aaaccatcat agtcgtcata gtgcgcttta atatcttcag caatatgctg ccagtcttcc
    21121 ggcgtcatat cagaagaatc catcaacggc gtatattcat gaatggtgaa atctggcatc
    21181 tccaggcgat ggaattccgg catcagcgcc agttggcgtt gtagatgacc tgacaccgga
    21241 atataaccct gctcggaacg ctgcatcccg atggtcccgc ccgtgtaggc gacgtaaatt
    21301 gatttctttt gcatgatatt gatatgaatg atatcgaaag agcaaaagta tatacgtgag
    21361 gcaggaggag cgcacctgat actatgcgga taaggaaata tggggcgaga cagcgggaag
    21421 aggattcgtc gaggtaaaaa ataatgtggc cgacgatctg tcggccactc agtacaagac
    21481 ttaacgcacg ttggcgcagg tcaggcaaaa cgcataacgg ttttgtgggt cgttaaacgc
    21541 ggccagcttg tcgctttcac ttttaacggt agaggctacc gaggcaagcg gtgcaggtaa
    21601 catggcctgg aacgcatctg gcaacattgc ccggacagaa ccagacatgt tgtccatcac
    21661 tttgtcgaag aaggtcggtt catcaacgta gtattccaga tgccactgtt tcacttttgc
    21721 gagctctgct gctttggcaa ccgcatcatc gaaatccccg agactatcga ccagcccgtt
    21781 agcttttgca tcctgaccgg tccagacgtg gccctgggcg attttatcaa tttgctccgg
    21841 cgtcgaatga cgcgcatcag caaccagcgt gataaagcgt ttatagccat tctcaatgct
    21901 taattgcatc atctgctgcg cttccggcgg cagtgccctg gtgatagaaa catccgccag
    21961 cggtgaagtt gagacaccat cagtatgaac accaatcgaa tccagactat tttctacggt
    22021 ggtgatcacg ccgaagatac cgatagaacc ggtcagggtg ctggggttag ccacaatgta
    22081 attagctggc gtggaaatcc agtaaccacc agatgccgcc atgccgccca tcgatacaac
    22141 cacagactta cccgctgccc gggctgctgc cagttcagcg cgaatcactt cagacgcggt
    22201 aacgctgccg cctgggctat taacacgcag gacaatcgct ttcactttcg ggtcaaggcg
    22261 agcgtcgcgg atttgtgccg cagtggtatc accgccaaca ttcccctgag tttcctcgcc
    22321 atccataatt gcgccattag cgaagacgac accgatgctg tcaccggtat ctgccggcgt
    22381 tttcaatgcg taatcgtaat aactgatggc gcgataattt ttatcagtct tactccagcc
    22441 gaactctttg gtcagagctt tttcgatttc ggcactcgat gccagtgcat cgaccagctt
    22501 gttttccagt gcatatttcg cggtatcgcc accggttttg gttaaaccct caagcaaccc
    22561 ttgcgcgcca gggaatacct gctgagcagg gatctgccgg ttagcggcaa cagtattcag
    22621 atagttttgc cacagctcac caatccagcg gctgtcagct tcgcgggctg ccggtgacat
    22681 atcatcacga ataaacggtt caacggcaga tttatacgta cccacgcgga acacatgggt
    22741 ggaaactttc agcttatcca gcaacgattt gtagtacaga ccgttggtag caaaaccgtg
    22801 cagatcaacc acgccttgcg gtgacagcca aattttattg gcgaaactgg cgagataata
    22861 ttgcccctgg ctgtagttct cgccaacggc ataaaccggt ttcccgctgt cacgaaactc
    22921 tttcagggct ttgccgatgt actgcataga cggctggtcg ccgcctgcga agtttttcag
    22981 atccatcacg ataccggtga tattgcggtc gtccttcgcc tggcgaatgg tgttgacgat
    23041 atcaaacagt gagttttcct gcagacgatc ggaactggca ccaagcagct ggcggcttaa
    23101 tttactaaac cgctgagaac tgtctggttt atcgacgatc acaccagaaa tgtccagcag
    23161 cagtgcgcca cgactggccg tttctttcga atcaccacca ctgacctgca tccagatccc
    23221 cacaccaacc agtacgagga aaataaagaa caggttaagt accatttcac ggacgaaatt
    23281 cagcagacgc cacgtccatt taaaaaatcc ggcaataaat cgccaaaggg ttcgcatgta
    23341 ttctcccaac ttaaggtcac acctgtcaca ggcgcaatag atgtggttat cgtaatgacc
    23401 caaccggcac ttgtcagcag gaattgcgga ctgtgctgta acaaaatcca ctctcgtgtt
    23461 aattttgtga ataaatatca cgacaggagt taatcaaatg gatgcactcg aactattgat
    23521 caatcgccgt agcgcctccc gcttggctga acccgcgcca acgggtgaac aactgcaaaa
    23581 catcctgcgt gcgggtatgc gtgcgccgga ccataagtcc atgcaaccgt ggcatttttt
    23641 tgtgattgaa ggggaagggt gcgagcgttt cagcgccgta ctggaacagg gggcgattgc
    23701 tgccggtagt gatgataaag ctatcgacaa agcccgtaat gcgccgttcc gcgcaccgct
    23761 catcatcacg gtggtggcga aatgcaaaga aaatcataaa gtcccgcgat gggaacagga
    23821 aatgtctgcc ggatgcgcgg tcatggcgat gcaaatggca gccgttgccc agggatttgg
    23881 cggcatctgg cgcagtggcg cattaactga aagtccggta gtgcgtgaag cattcggttg
    23941 ccgtgagcag gataaaattg tcggttttct ctacctcggc acgccgcagt tgaaagcatc
    24001 tacgtcgatt aacgtcccgg acccgacgcc gtttgtaact tatttctgat aattgtcgcg
    24061 aaactgtctg gattgtgagc aaaggcgcag gaattcagac actctcactt atcacttcac
    24121 ggaatgaggg ttaccatacc cggattgcat tgacaggaga tgtccatgag cgagaactcg
    24181 attcgtttga cccaatacag ccacggagct ggttgcggct gtaaaatttc cccaaaagtg
    24241 ttggaaacca tcctgcacag tgagcaggcg aagtttgttg atccgaattt gcttgtgggt
    24301 aatgaaaccc gcgacgatgc ggcggtgtac gatctgggca atggcaccag cgttatcagt
    24361 accaccgact tctttatgcc gatcgttgat aatcctttcg attttggccg cattgcggcg
    24421 actaacgcca tcagcgatat cttcgcgatg gggggcaaac cgattatggc gattgcgatc
    24481 ctcggctggc cgattaacaa actttccccg gaaattgccc gcgaagtaac cgaaggtgga
    24541 cgctatgcat gccgtcaggc gggtattgcg ctggctggcg gtcactccat cgatgcgccg
    24601 gagccgattt ttggtctggc ggtaacgggg atcgtaccga ccgagcgggt gaagaaaaac
    24661 agtaccgcac aagccggatg caaactgttc ctgacgaaac cgctggggat cggcgttctt
    24721 accacggctg agaaaaaatc actgttgaaa ccagaacatc agggactggc gacggaagtg
    24781 atgtgccgga tgaacatcgc aggcgcgtcc tttgccaaca tcgaaggcgt aaaagcgatg
    24841 accgacgtta cgggctttgg tctgctgggc cacttgagcg aaatgtgtca gggggctggt
    24901 gtgcaggcac gcgtcgacta tgaagcgatc ccgaaactcc ccggtgttga agagtacatt
    24961 aagttgggcg cagtacctgg cggcactgaa cgtaactttg ccagctacgg tcatctgatg
    25021 ggtgaaatgc cgcgtgaagt gcgcgatctg ctgtgtgatc cgcaaacttc tggcggtttg
    25081 ctgctggcgg tcatgccgga agcagaaaat gaggttaaaa ctacagccgc cgagtttggc
    25141 attgaactga cggcgattgg cgaactggtg ccagcgcgcg gcggtcgtgc catggttgag
    25201 atccgttaat tcaatgcggt tgtttattgc cgaaaaaccg agtctggcgc gcgccattgc
    25261 tgatgtcctg cccaaaccgc accggaaagg cgatggcttt atcgagtgcg gtaatggtca
    25321 ggtggtgact tggtgtatcg gtcacctgct tgagcaggcg cagccagacg cctacgacag
    25381 ccgctatgcg cgctggaatc ttgcggattt gccgattgtc ccggaaaagt ggcaattaca
    25441 gccacgaccc tccgtgacca aacaacttaa cgtcatcaaa cgcttcctgc atgaagccag
    25501 cgaaatcgtt cacgccgggg acccggatcg tgaagggcaa ttgctggtgg atgaagtgct
    25561 ggactatctg caactggcac cggaaaagcg ccagcaggtg cagcgttgct tgataaacga
    25621 cctgaacccg caggcggttg agcgggcgat cgaccgtctt cgttccaaca gtgagtttgt
    25681 gccattgtgc gtttctgcgc tggcgcgagc gcgtgccgac tggctgtacg gcatcaatat
    25741 gacccgtgcg tataccattc tcggtcgcaa tgccggttat cagggcgtac tttccgtggg
    25801 acgcgtgcag acacccgtgc tcgggctggt ggtgcgccgc gatgaagaga ttgaaaactt
    25861 cgtggcgaaa gacttctttg aagtcaaagc acatatcgtg acacctgccg atgagcggtt
    25921 taccgctatc tggcaaccga gcgaagcgtg tgaaccgtac caggatgaag aagggcgctt
    25981 gttacatcgt ccactggcgg agcatgtggt taaccgcatt agtggtcaac cggctattgt
    26041 caccagctat aacgataaac gggaatcaga atccgcgccg ctgccgtttt cgctttcggc
    26101 gttgcagatt gaagcggcaa aacgcttcgg tctgagcgcg cagaacgtgc ttgatatctg
    26161 ccagaagctg tacgaaacgc acaagctaat cacttatccg cgttccgatt gtcgctattt
    26221 gccagaagaa cattttgccg gacgccacgc ggtgatgaat gccatcagcg ttcatgcacc
    26281 ggatctgttg ccgcagccag tggtagatcc agatatacgc aaccgctgtt gggatgacaa
    26341 aaaggtcgat gcgcaccacg ccatcatccc gaccgcacgg agttctgcga tcaacctgac
    26401 ggagaacgaa gcgaaggtct ataacctgat tgcccgtcaa tacctgatgc agttctgccc
    26461 ggatgcggtg ttccgcaagt gtgttatcga actggacatt gccaaaggca aatttgtcgc
    26521 taaagcccgt tttcttgctg aagcaggctg gcgcacgctg ttaggcagca aagagcgcga
    26581 tgaagaaaac gacggcacgc cattgcctgt ggtggcgaaa ggcgatgagt tgctgtgtga
    26641 aaaaggtgaa gtggtagagc ggcaaaccca gccgccgcgc cattttaccg atgcaacact
    26701 gctttcggcg atgaccggga tcgcgcgctt tgtgcaggac aaagatctga aaaaggtcct
    26761 tcgtgcgacc gatggtctgg ggacagaagc aacccgtgcc gggattattg aactgctgtt
    26821 caagcgtggt tttctgacca aaaaagggcg ctatatccac tccaccgacg ccggaaaagc
    26881 gttattccat tcgctgccag aaatggcgac gcgaccggac atgaccgcgc actgggaatc
    26941 ggtgctgacg caaatcagcg aaaagcagtg tcgctatcag gactttatgc agccgctggt
    27001 ggggacgcta tatcagctta ttgatcaagc caaacgtacg ccggtgcggc agtttcgcgg
    27061 cattgtggct ccgggcagtg gtggcagtgc tgataagaaa aaggctgcac cgcgtaaacg
    27121 tagtgcgaaa aaaagtccgc cagcagatga agccggaagc ggggcgatag cgtaagcgag
    27181 cgaatctttc gtgctattcg agtcatattc tgaaatatcc agcggatcaa gaaaattcgt
    27241 tggatatttt tttgcatgga taaaattatc gcctctaaag tatgtaataa cagggaatgt
    27301 gatgaaaaaa gttttattac aaaaccatcc tgggagcgag aagtattctt ttaatggctg
    27361 gggaatattt aatagtaatt ttgaacgggt gattaaagaa aataaggcca tgctgctttg
    27421 taagtggggg ttttatttaa catgtgttgt cgctgtaatg tttgtattcg cagcgataac
    27481 atccaacggt ttgaatgaaa gaggcctgat taccgcggga tgctcttttc tttatctatt
    27541 aattatgatg gggcttattg ttcgggccgg ttttaaagca aaaaaagaac aactacatta
    27601 ttatcaggct aaaggtattg agccgctcag tatcgagaag ttacaggcgc tacaattgat
    27661 cgcaccttat cgattctatc ataagcaatg gtctgaaacg ctagagttct ggccgcgaaa
    27721 gcctgaacct ggcaaagata ccttccaata tcatgtgctt ccttttgact cgatcgatat
    27781 catcagtaaa agacgcgagt ctttagagaa tcaattgggt atcgaagata gcgaaagtta
    27841 ttgtgcctta atggagcatt ttctttctgg cgaccatgga gccaatacct ttaaagcaaa
    27901 catggaggaa gccccagagc aggttatcgc cgtgttgaat aaatttgctg tttttccctc
    27961 agactatatc tctgattgcg ctaataatcg ctccggtaaa tcctcggcga agctaatatg
    28021 ggcggcggaa ttatcatgga tgatctcgat atcaagcaca gcttttcaaa acgggacaat
    28081 tgaagaagaa ctggcctggc attatataat gcttgcttct cgaaaggcgc acgagttgtt
    28141 cgaaagcgaa gaagattatc aaaaaaatag tctaatggga tttctttact ggcatatctg
    28201 ctgctatcgc agaaagttaa cggatgctga acttgaagcg tgttatcgtt acgacaagca
    28261 gttttgggag cactacagta agaaatgccg ttggcccata agaaatgttc cctggggagc
    28321 atcatccgtt aaatactcat aaacgcctga aattttgccg ggggcgctgc gcttgcccgg
    28381 cctacattag agcgtaactg caatgaattg tgcccatctg taggcctgat aagcgtagcg
    28441 ccatcaggca tttacaactt aaatcacacc ctgcgccagc atcgcatcgg caaccttcac
    28501 aaaaccggca atgttcgcgc cctgcacgta gttggtttgc tcaccttcac caccatgctc
    28561 aacacaggca tggtggatat ccagcatgat gtgatgcaaa cgtgcgtcaa ctttctcggc
    28621 tttccagccc aggcgcgcag cgttttgtgc catttccagg cccgacgttg ctacaccgcc
    28681 agcattagcc gctttacccg gtgcaaacag tacgcctgcc tgctggaaca gttcagtcgc
    28741 ttcgatggtg gtcggcatat ttgccccttc agcgacggct ttaacgccat tggcgataag
    28801 ctgatgcgcg gtgtcaacat ccagttcgtt ctgggtggcg caaggcaggg cgatatcaac
    28861 cggtacagac cacggctgtt ggccttcgag atagaccaga ccaaattctt tggcgtaatc
    28921 tgccactcga ccatcgcggc tggctttgat ttcgataaga cgtgccagtt tctctttcgt
    28981 gaatccgctt tcatcaacta cagtgccgct ggagtctgac gcagtgatca cacgagcacc
    29041 aaattccatc gctttttcga tagcgtactg ggcgacgttg ccggagccag aaacggaaac
    29101 gcgcatccct tcaaaaccca taccgtggcg ttttaacatt gcttctgtga aataaaccag
    29161 accgtagccg gtagcttccg ggcgaataag actaccgcca aatgaaaggc ccttaccggt
    29221 gaagacgcag gcggtattgt tggagagctt tttcatcatc cccgccataa agccgacttc
    29281 acgaccacca acgccgatat cacctgccgg aacgtcggta tccgcgccca ggtggcgata
    29341 cagttcagtc atcagcgcct ggcaaaaacg catcacttta ccttcgcttt ttcctttcgg
    29401 atcgaaatcg ctgccgcctt taccaccgcc catcggcagg gtagtcaggg catttttgaa
    29461 ggtttgttca aagccgagga atttgagaat ggaaaggtta actgacggat ggaagcgcat
    29521 accgcctttg tacgggccga tggcagagct gaactgcaca cgccatgcac ggttgacctg
    29581 tatctggttg cgatcatcaa cccataccac gcgaaactgg atcacgcgct ccggttcaac
    29641 cagacgctcc agtaatgaca tctggcgata ttttggattt tgttcaagaa aaggccagag
    29701 tgtggtcatt acttcacgaa cggcttgcgc gaactcggtt tgattcgggt cgcgcttttg
    29761 gacatggttg aggaatgact ccagagaata tgtctgatcc atagatataa aaaccttata
    29821 tattaatacg attgtgctta tgttgtttat gtcatgtgct tttgcagttt tcgactatac
    29881 catcaagaat gtgacggtaa gcaagaaaaa aatgacccag gaaagcaaat taataacgag
    29941 agtaatctca taaccaaatg cgataactca gacgctaaag ttttgccacc cggctaccgt
    30001 tatcagtagc agagacaata acgttaagga gttccctgtg agtcgagcat tgttcgccgt
    30061 agtattagca tttccattaa ttgcactggc taacccgcat tatcgtccgg acgtggaggt
    30121 aaacgtaccg ccagaagtgt tcagttcagg cgggcagtca gcgcagccct gtactcagtg
    30181 ctgcgtttat caggatcaaa attattctga aggggcggta attaaagccg aaggaatact
    30241 gctgcaatgc cagcgtgatg acaaaaccct tagcactaat ccgctgctct ggcggcgcgt
    30301 aaagccataa acgcctctaa taatggaatg tcagcagggg ccagcggata ttgcagcgcc
    30361 tcttcaggtg agcaccagac cagcgcctga tgttcatgtg cctgtaacgt cccgtggaag
    30421 tcgggtacgt gccaggcatg aagatggata atccggcccg aaacttctcg ctgatggctg
    30481 gcaacatact cacccacagt tgcttcgatg tccaattctt cgtttaactc acgcaccagc
    30541 gcctgctgct ggctttcatc cagctcgact ttacctccgg caaactccca taatcccgcc
    30601 tgatcgctct gggcggggcg ttgcgcgagt aaaattttgc catcacgttc aatgatggcg
    30661 gcaacaactt caatcatttt cataaattac acacgggcag ataagcgaaa aatgccataa
    30721 tatccgcact gttgctcaat cagaaaggct ttatatttaa ggagtgcctg tgctagaccg
    30781 ccatcttcat ccccggatta aaccgttgtt gcatcagtgc gtgcgggttc ttgataaatc
    30841 gggcattacg ccagatggtt taacattagt gggatttgcc atcggcgtgc tggctctgcc
    30901 gtttctggcg ctgggctggt atctggcggc gctagtcgtc attttgttga acaggctgct
    30961 tgatggtctg gacggtgcgc tggcgcggcg cagagggctt accgacgcgg gcggctttct
    31021 tgatatttct ctcgattttc tcttttacgc gctggtgccg tttggcttta ttcttgctgc
    31081 accggagcaa aatgcgctgg caggcggctg gttgttgttt gcgtttatcg gcacgggcag
    31141 cagttttctc gcctttgctg cactggcagc aaaacatcag attgataacc ccggttatgc
    31201 acataagtcg ttttactatc tggggggatt aaccgaaggc acagaaacga tcttactgtt
    31261 tgtgctggga tgtttattcc ctgcatggtt tgcgtggttt gcgtggtttg cgtggtttgc
    31321 gtggatcttc ggtgcgttgt gctggatgac gacctttacg cgggtgtgga gtgggtatct
    31381 gacgctgaag tcgctccagc gtcagtaatt gcaatgttat ttgctactgc ccgggccgcg
    31441 ttcaccggtt gcaaccggat tttttggatc gctgctccat tcgtaccagc cgccgtcata
    31501 cacggaaaca ttcttccaac ccatggcgcg tgcgtacata aaggtttcgg acgcgcgcca
    31561 gccggtgccg cagtagaatg aaacttgctg atctggtttg atattccatg ctttccacat
    31621 agcggtaata tcatcggcgc tacgcatggt gccatccggg ttatggaaat cttccatatg
    31681 cgtcgagtcg ctaccagcgt gtccccaacg tgctccggct atttcacctt ttggtttaat
    31741 atagctgtaa ccgctggtcg taccgataaa ttctggccac gaacgaatgc tcaccagcga
    31801 tgcatcctgg cgatgcagca gtccacgggc ctgttccata tcgagcatca actgcggttg
    31861 tgccgggatc ttcacgccga aatctggttc cgctttcact ttcggtggcg ttcctcgctc
    31921 aacaggcagt cccgcgtcgg accaggtttg ccagccgcca tccagcagac gcacatcttt
    31981 cacgcctgcg tagagcataa tttgcgccac acgcgctcca gcgtatacgt cacgcccgta
    32041 cagaatgacc gtggtgtcat ggcgaatgcc gtgttttgcc agcatcgctt ttagttgttc
    32101 atcagaaact ttgttccaca gcggttcgct ttccacttcg ttggtatcga tgtagtcagc
    32161 gccaggaata tggctgataa ggtaaagctt aggagcgccc caggccgctt caatgacttt
    32221 ccagccaccg acaggtttcg ccgtaacctc tttaccttgt tgcaggtcat gcagccattg
    32281 cggataaacc agctgctcaa agtgcggcag tttttgtaga cgggaaggtt cgcttagcgc
    32341 gtcactcaga atggagatat gcgtaaaacc tgctttttgc agtcgcgttt tgacggcgtc
    32401 gacatctttg ccattaccgt acagcgccac cggggcatcg gctttcaggt tatgttgctt
    32461 gatccacgcg ttgagctgtt cggtgctcat tttgtcgagc cagctagcag agaggtttaa
    32521 ggcggcaggt tcatgaccag aagggccatt taaggtttgt ggccagccgt tataaaacgc
    32581 gctggggcga gtatctatcg ctttgccatt ttgttgctga agttggtcaa gtgtaagagg
    32641 cttcgccagt tcagcggccc acgaagaagc gcaagccagc cctaaagcca ttgccagcgc
    32701 ggtcatttga gaaacacgtt tcatcgaaaa tcccgacatt atagttttga gtgcgccatt
    32761 ctgaactgac gcattgagaa aaactttgcg ttatcgcagt ttgttgtaat tttctgacca
    32821 ctgcgccata tccagaacag aactatcagc aggaacatcc tggagatcgt gcgttacctg
    32881 aacgacgggg atcgccaggg cgcgaacttc gctgaacacc cactggcgaa aattatcgcg
    32941 cagagccaca tcaagacggc tgaatggctc atccaggagt aacgcttttg gctgggcgag
    33001 aagggcgcgt agtagagcaa cgcgcgctcg ctgaccgcca gacaaagtgg caggatcctg
    33061 atggaaagct ccgtcaaggc ctgaacgctc aagtgcatca ttcacggcat tacgtcgggc
    33121 attcccctta agtgtcgccg gtagcgccag cagtaaattt tgcccgacgc tgaactggtc
    33181 aaataacagt gcatcctgaa aaagaatgcc aatctgacgc tgtgcggtgg gtagcatgtc
    33241 aatccgttgc tcattgagcc atagctcacc tgtacaagaa aactgtccgg ccagtgcacc
    33301 aatcatccat gaaaacagag tggattttcc acagccagat ggccccatta acgtgacgat
    33361 gtcaccttta tcaaccgtaa agttaacgtt tgtcagcaag cggctttctg gtaaacgtag
    33421 cgaaacattt ttcacgcaga gcattagcgg attccttgtc tgacataacc tacccatttt
    33481 gcgactaacg cggtcagggc aaaaataata agcggtaaca acagttgcca taaagcctgg
    33541 gcggcgagaa taccgttgct gccgccgctg cttaatgcca ccgcctcagt ggtgagcgtc
    33601 ggaaaacgcc ccgcgcccag ccacagcgtc ggcatatact gcgcaatact gactgaaaat
    33661 cccaccgcga aggcaatcag cacagggcgc aacatgagtg ggcatttcac gtaaaagaag
    33721 attttggccc gcgaccagcc cagtgtttgc gcaatcaaaa ttaaccgtga atcaatgcgc
    33781 tgccaggcgg gttgcaggat aaacagcatc cacggcatta cccacagcag atgcccccag
    33841 accaccgccg tccagcttcc atccaggttc agccatagcg ccagcgtgta ctagcccgcc
    33901 accagtggca gagcgggcaa tagaatgggc aaccatagcc acaactggcg acgctgtgcc
    33961 ccccattcca gccacagcag taacaggagc aaagcgatga atgttgctac cagccccatt
    34021 gtcagactgt tgatgagtgc ttcactattg atcgtcgact gatccgcgag gatcgccagc
    34081 agaaccacac acagcacacc ggttaagggt aaaaaactcg ccagcgtagt gcctggtaat
    34141 aaaggtgtgg caggcttgcg aatgccatct acgcggggaa tagtgcgccg ccagctgcgc
    34201 catagcagat agctcagcaa aacgtaggcg gcgagtaaca gcatcagcag cagactggcg
    34261 agtgcgcctt tcgtttgttg atccgcgtcg ccctgagtta accactgcca gctaattact
    34321 gccagcgtcg gtggattacc tggcccaaga ataatcgcca catccacgac cgaaagcgac
    34381 caggcaacaa tcgccagcat cgccattgcc agcgaaggcg cgacggaggg caacagcagc
    34441 cagttcaggc attgccagcg gctgtagccc agcgaatcca gcacaatgac ctgctgcaac
    34501 agccgttttt cgctcaatac tgccactaag atccacagca gaaatgcgct ttctttcact
    34561 gcaagggtga ggcccagacc gatccaaatc ggtccattgg cggagtgaaa tacgggaaat
    34621 agtcatatag cagccctccg tcagcaaaga gcagcagggc gctggtggca aaagccacat
    34681 ggggaatggc gagcagccac ggcagacggg cgcacatacg ctgccatttc ggcccaggcc
    34741 atagcgccac aatcaccagc agggcaatca acaatgcccc gaccgccgcg atggttgtcg
    34801 acaccagcgt tgccagtaat gcctgcggta actgtggatc ggcaaataac gcctgccagc
    34861 gtgtcaacga caacgcaggc gatatcagcg tgagagctgc cgggatcagc ggtgcataaa
    34921 tcaccgccac catcgcccac agcaaaaaga ttaatgcgta ccgtaacggc gtaaccattc
    34981 ttgttccagt gcattcaccc atcctgcgtg cggttcagcc agtaccggcg gcagatcctg
    35041 cggcattctt gattgcaata tttcgcgctg cccgtcaggc agtttttgcg gatcgagaac
    35101 agaaggatcg ccccagacaa cgggatctgc tttacgcagt tgcgcatcgg gtgagagcag
    35161 gaaattggca actaccttcg ccgcagcact ggcattcgcg ttggctggaa tggtgacgaa
    35221 atgcacgttg ccaatcatcc cctcgcgaaa gccaaaactg taactgcttg ccggcaaatc
    35281 gccgctggcg attttttgct gcgcatgcgc ggggttaaag gtcagcgaca ggcgcaatgt
    35341 gccggctttc agcagagcat ccatccgcgc gggcgaaggc gggaaatcct ttccttcgcg
    35401 ccacaaatac ggatgcagtg catcaagata ttgccacaag ggcgcagtga cacgggcgaa
    35461 agtcgcatcg tccggcgctt cttttaatgc ggcgggatct ggcgtcagca taatcagcaa
    35521 ctgttcaaga aacgccgtgc cggtaaagtc cggtgggcgc ggataagtaa ccgtgccggg
    35581 attggcttta gcaaactcca gtaaggcttg cggcgtttgt ggtggctgtg gcgtaacatc
    35641 gcggcgggcg ataaacgtca gttgtgcgcc gccccagggc gattccgcac cttgtgtcgg
    35701 cactgaaaaa tcttcccgca ccggcagctg tgtgtcgaca tagcgccagt tgggcagagt
    35761 ctccgcccag cccgtttgta ataaattggc ctctttta
//
LOCUS       DADMGC010000026        35290 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.026, whole genome shotgun sequence.
ACCESSION   DADMGC010000026 DADMGC010000000
VERSION     DADMGC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35290
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.026"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..1151)
                     /gene="setB"
                     /locus_tag="JNZ73_001275"
     CDS             complement(<1..1151)
                     /gene="setB"
                     /locus_tag="JNZ73_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HAY8731615.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EIHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATL"
     gene            1519..2649
                     /locus_tag="JNZ73_001276"
     CDS             1519..2649
                     /locus_tag="JNZ73_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HAY8731616.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNCIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2649..3587
                     /gene="fruK"
                     /locus_tag="JNZ73_001277"
     CDS             2649..3587
                     /gene="fruK"
                     /locus_tag="JNZ73_001277"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAY8731617.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3604..5295
                     /gene="fruA"
                     /locus_tag="JNZ73_001278"
     CDS             3604..5295
                     /gene="fruA"
                     /locus_tag="JNZ73_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HAY8731618.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5719..6660
                     /locus_tag="JNZ73_001279"
     CDS             5719..6660
                     /locus_tag="JNZ73_001279"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HAY8731619.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYVDSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSASKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6648..7586
                     /locus_tag="JNZ73_001280"
     CDS             6648..7586
                     /locus_tag="JNZ73_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HAY8731620.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQIAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7680..8930
                     /locus_tag="JNZ73_001281"
     CDS             7680..8930
                     /locus_tag="JNZ73_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY8731621.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFIA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9001..9699)
                     /gene="yeiL"
                     /locus_tag="JNZ73_001282"
     CDS             complement(9001..9699)
                     /gene="yeiL"
                     /locus_tag="JNZ73_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HAY8731622.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLMNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            9830..10771
                     /gene="rihB"
                     /locus_tag="JNZ73_001283"
     CDS             9830..10771
                     /gene="rihB"
                     /locus_tag="JNZ73_001283"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HAY8731623.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMAGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10871..12121
                     /locus_tag="JNZ73_001284"
     CDS             10871..12121
                     /locus_tag="JNZ73_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY8731624.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLVLNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12177..13265)
                     /locus_tag="JNZ73_001285"
     CDS             complement(12177..13265)
                     /locus_tag="JNZ73_001285"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY8731625.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLVDCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13268..14124)
                     /gene="nfo"
                     /locus_tag="JNZ73_001286"
                     /pseudo
     CDS             complement(13268..14124)
                     /gene="nfo"
                     /locus_tag="JNZ73_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
     gene            complement(14198..15247)
                     /locus_tag="JNZ73_001287"
     CDS             complement(14198..15247)
                     /locus_tag="JNZ73_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HAY8731626.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15346..16227
                     /locus_tag="JNZ73_001288"
     CDS             15346..16227
                     /locus_tag="JNZ73_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8731627.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16433..17902
                     /gene="lysP"
                     /locus_tag="JNZ73_001289"
     CDS             16433..17902
                     /gene="lysP"
                     /locus_tag="JNZ73_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HAY8731628.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVLAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18194..20185
                     /gene="cirA"
                     /locus_tag="JNZ73_001290"
                     /pseudo
     CDS             18194..20185
                     /gene="cirA"
                     /locus_tag="JNZ73_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20217..21053)
                     /locus_tag="JNZ73_001291"
     CDS             complement(20217..21053)
                     /locus_tag="JNZ73_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase YeiG"
                     /protein_id="HAY8731629.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            21311..21979
                     /gene="folE"
                     /locus_tag="JNZ73_001292"
     CDS             21311..21979
                     /gene="folE"
                     /locus_tag="JNZ73_001292"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAY8731630.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21996..23153
                     /locus_tag="JNZ73_001293"
     CDS             21996..23153
                     /locus_tag="JNZ73_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HAY8731631.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWWLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGLAISKTSR"
     gene            23295..24263
                     /gene="galS"
                     /locus_tag="JNZ73_001294"
     CDS             23295..24263
                     /gene="galS"
                     /locus_tag="JNZ73_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HAY8731632.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRAATVLCRR"
     gene            24614..25612
                     /gene="mglB"
                     /locus_tag="JNZ73_001295"
     CDS             24614..25612
                     /gene="mglB"
                     /locus_tag="JNZ73_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HAY8731633.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25673..27193
                     /gene="mglA"
                     /locus_tag="JNZ73_001296"
     CDS             25673..27193
                     /gene="mglA"
                     /locus_tag="JNZ73_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HAY8731634.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIGMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27209..28219
                     /gene="mglC"
                     /locus_tag="JNZ73_001297"
     CDS             27209..28219
                     /gene="mglC"
                     /locus_tag="JNZ73_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HAY8731635.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28290..29525)
                     /gene="preA"
                     /locus_tag="JNZ73_001298"
     CDS             complement(28290..29525)
                     /gene="preA"
                     /locus_tag="JNZ73_001298"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HAY8731636.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVKKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(29519..30759)
                     /locus_tag="JNZ73_001299"
                     /pseudo
     CDS             complement(29519..30759)
                     /locus_tag="JNZ73_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(30953..31192)
                     /locus_tag="JNZ73_001300"
     CDS             complement(30953..31192)
                     /locus_tag="JNZ73_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HAY8731637.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31195..31914)
                     /gene="sanA"
                     /locus_tag="JNZ73_001301"
     CDS             complement(31195..31914)
                     /gene="sanA"
                     /locus_tag="JNZ73_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HAY8731638.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(32064..32948)
                     /gene="cdd"
                     /locus_tag="JNZ73_001302"
     CDS             complement(32064..32948)
                     /gene="cdd"
                     /locus_tag="JNZ73_001302"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAY8731639.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(33078..33773)
                     /locus_tag="JNZ73_001303"
     CDS             complement(33078..33773)
                     /locus_tag="JNZ73_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HAY8731640.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33770..34168)
                     /locus_tag="JNZ73_001304"
     CDS             complement(33770..34168)
                     /locus_tag="JNZ73_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAY8731641.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34407..>35290
                     /gene="dusC"
                     /locus_tag="JNZ73_001305"
     CDS             34407..>35290
                     /gene="dusC"
                     /locus_tag="JNZ73_001305"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HAY8731642.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVR"
BASE COUNT         8664 a         8625 c         9321 g         8680 t
ORIGIN      
        1 agagtggcga taatcatcac catcgcaaac cagaacacag cgtgataatt ccagatctcg
       61 gcgacgatgc ccgccactga tcctgcgatg atccagccca cgcgcgacgt gttggtatag
      121 agcgtggtgg ctgaacctgc ctgaccgggc atcaaatcct gaaaatagag catcccgatg
      181 ccgcccagaa tgccaataaa aatagcattt agcagctgca agcccaacag aatggcaggt
      241 gaatgcgcca tcagcattcc tgcgtaaaaa cagacgccac ccacggcagc aacgcgcatt
      301 aagaaacgct tacccagacg tttggcgaaa tatccggcaa tcaacatcgt cgggatttcc
      361 agcccggcgg cggtccccat catcacaccg gccagtttct cgggaagatg cagttcgttg
      421 ataataaata gcggcatgtt gatgatgtac aggctgttcg agccccacat caatgtacaa
      481 atgacaaaca gcagtagcgt atcgcgacgg ttacggcgcg gcgcttcaac cgtgccggtc
      541 gccagcggaa gctctttttg catcgacggt aaaaacagcc acaccatcac gccgcaaaca
      601 ataaacgcta ccgctgcgct cagatacatt accgtaaagc tgaaacccat cgctaacgca
      661 taagccagcg gtgggccaat gacccatgcc agtgaaacct gagcgcgtaa aaaagagctg
      721 aacatcaccg cctcacgtcc ggttttgtcg gcatgttcac gggcaagggc aaacatttgc
      781 gggttagcgg tcgagccaaa gctgctaaga aagacgccaa cgaatagcaa aacaaagtag
      841 ttgcgattcc aggcaaaaag ggtgcaggcc agcacgccta acaggcagca aaagacaatc
      901 agcgatttgc gatcgccgcg cttatcagag cgcccagcga gaaactgact taccagaatc
      961 ccaatgacag cgctgccggt aaagaagaat cccaccatcg ccggacgggc atgtatttca
     1021 tcggtaagga aaatactgag tgtcggggtt tgcagagcac ccgcaatacc ggtgagaaag
     1081 gcaacgatta aaaacgccgt cgaggtcagg tcaaacgatt tcgcgctgga gactgcgggg
     1141 gagttatgca tgctggtcga tcactggaag aggaagacgc ggagtttacg ctgcttgtca
     1201 gaaaataaac agacagcgaa atcaaaagcg ccacgaataa tcaaaatgcc atttcactgt
     1261 tgctgattgg ctgctgacgc tgctgaaatg aaattgctga taacggattt tcccatcagc
     1321 aattaggaaa aatgacaaaa aattgtgcag cacatcaaac ttttgctcat aactttacgg
     1381 ctttccttgc gtgctgaaac agaagtatta tgctttcttg aaacgtttca gcgcgatctt
     1441 gtctttaacc ctaagccagg ttggcgcttt ttttctcata gaggctgaat cgtttcaatt
     1501 cagcaagaga ggagaactat gttccagtta tccgtacagg acatccatcc gggcgaaaag
     1561 gccggagaca aagaagaggc gattcgccag gtcgctgcgg cgctggtgca ggccggtaat
     1621 gtagcagaag gctacgtcaa tggcatgctg gcgcgcgaac agcaaacctc aacgttcctc
     1681 ggcaattgta ttgctattcc acacggcact accgacaccc gcgatcaggt gctgaaaacc
     1741 ggcgttcagg tatttcagtt cccggaaggc gtcacctggg gtgacggtca ggtagcgtac
     1801 gtggcaatcg gtattgctgc cagctcggat aaacatctgg gcctgctacg ccagctgacc
     1861 cacgtactga gcgatgattc cgttgctgaa caactgaagt cagcaacaac agcagaagaa
     1921 cttcgcgcat tactgatggg cgaaaagcag agtgagcagc tgaagctcga caacgaaatg
     1981 ctgacgctgg atatcgtcgc cagcgatctg ctgactcttc aggcgctgaa cgctgcgcgt
     2041 ctgaaagagg cgagggcagt tgacgccact ttcgtcacca aagccatcaa tgaacaaccg
     2101 ctgaatctcg gacagggtat ctggctgagc gatagcgccg aaggcaatct gcgtagtgcg
     2161 attgcggtaa gccgtgcggc aaatgctttt gatgtggacg gcgaaacggc agccatgctg
     2221 gtgagtgtgg cgatgaatga cgatcagccc atcgcggttc ttaagcgtct cgctgatttg
     2281 ttgctcgaca ataaagctga ccgcttgctg aaagcggatg cggcaacgtt gctggcgctg
     2341 ctgaccagcg atgatgcgcc gaccgacgac gtgttaagcg cggagtttgt ggtgcgcaat
     2401 gaacacggcc tgcatgctcg tccaggtacc atgctggtca ataccattaa acaatttaac
     2461 agtgatatta ccgtgacaaa ccttgatggc accggcaaac cggcaaacgg acgtagtctg
     2521 atgaaagttg tggcacttgg cgttaagaaa ggtcatcgcc tacgctttac cgcccagggt
     2581 gcagatgctg aacaggcgct gaaagccatt ggcgacgcta tcgctgctgg tcttggggag
     2641 ggcgcataat gagcagacgt gttgctacga tcacccttaa tccggcttat gaccttgttg
     2701 gtttctgccc ggaaattgaa cgcggcgaag tgaacctggt gaaaaccacc ggtctgcatg
     2761 cggcgggtaa aggcatcaac gtggccaaag tattaaaaga cctgggaatt gatgtcaccg
     2821 ttggcggctt cctgggtaaa gacaatcagg atggttttca gcaactgttc agcgagctgg
     2881 gcattgccaa ccgtttccag gttgtacagg ggcgcacccg aattaacgtt aagctgacgg
     2941 aaaaagacgg cgaagtgacc gacttcaact tctcgggttt tgaagtcacc cccgccgact
     3001 gggaacgctt tgtgactgat tctctgagct ggctcggtca gttcgatatg gtctgtgtca
     3061 gcggaagctt accgtcaggc gtcagcccgg aagcgttcac cgactggatg actcgcctgc
     3121 gtagtcagtg tccttgcatt atctttgata gtagccgtga agcgttagta gcaggtttga
     3181 aagcggcacc gtggctggtg aaacctaacc gccgcgagtt ggaaatctgg gcaggccgta
     3241 aactgcctga aatgaaagat gtgattgaag ctgcacatgc gctacgtgaa caaggtattg
     3301 cgcatgttgt tatttcactg ggtgccgaag gcgcgctttg ggttaatgcc tccggcgaat
     3361 ggatcgctaa accaccgtca gtcgatgtcg taagcaccgt tggcgcaggg gattctatgg
     3421 ttggtggcct gatttatggc ttgctgatgc gtgaatccag tgaacacaca ctgcgtctgg
     3481 cgacagctgt tgcagccctg gcggtaagtc aaagcaatgt gggtattacc gatcgtccgc
     3541 agttggccgc aatgatggcg cgcgtcgact tacaaccttt taactgacag caggagaggc
     3601 ataatgaaaa cgctgctgat tattgacgct aatctcggtc aggcacgcgc ctatatggcg
     3661 aagaccctgc tgggcgcggc ggcgcgaaaa gcaaaactgg aaatcatcga caatccgaac
     3721 gacgcagaaa tggcgattgt tctcggtgat tccatcccga atgacagcgc gctgaacggt
     3781 aaaaatgtct ggctgggtga tatttcccgg gcagttgcgc accctgagct gttcctgagt
     3841 gaagccaaag gccatgcgaa accttacact gcgccggtca ctgcgacagc accggttgcc
     3901 gccagcggtc cgaaacgcgt agttgcggtg actgcttgcc cgactggcgt agcacacacc
     3961 tttatggcgg ctgaagccat tgaaaccgaa gcgaaaaaac gtggctggtg ggtgaaagtt
     4021 gaaacccgtg gttctgttgg cgcgggtaat gcaatcactc ccgaggaagt agccgcagcg
     4081 gatctggtga ttgtggcggc agatatcgaa gtggatctgg cgaaatttgc tggtaaaccg
     4141 atgtatcgta cctctaccgg tctggcgctg aagaaaacct cgcaggaact ggataaagcg
     4201 gttgctgaag caacgccgta tgaaccggcg ggcaaagctc aaacggcgac cactgaaggt
     4261 aagaaagaga gtgcaggcgc ttatcgtcac ttgctaacgg gcgtctctta catgctgccg
     4321 atggtcgttg ctggtggtct gtgtatcgcg ctttcttttg cttttggtat cgaagcgttt
     4381 aaagagccgg gtacgttggc agcggcgctg atgcagattg gtggtggttc agcctttgcg
     4441 ctgatggtgc cggtactggc aggttatatt gccttctcca ttgccgatcg tccgggcctc
     4501 acgccgggtc tgattggcgg tatgctggcg gtcagcaccg gttctggctt cattggcggt
     4561 attattgcgg gcttcctggc tggttacatt gcgaagttaa tcagtacgca actgaaactg
     4621 ccgcagagta tggaggcgct gaaaccgatc ctgatcattc cgctaatttc cagtctggtg
     4681 gtcggtctgg cgatgatcta cctgatcggt aaaccagttg ctggcattct cgaagggctg
     4741 actcactggc tgcagaccat ggggactgcg aatgcggttc tgctgggggc gatcctcggt
     4801 ggcatgatgt gtactgacat gggcggtccg gtaaacaaag cagcgtacgc attcggtgtg
     4861 ggtctgctga gtactcaaac ctatggcccg atggcggcga ttatggcggc aggtatggtg
     4921 ccaccgctgg caatgggcct ggcaacaatg gtggcgcgtc gcaaattcga caaagcgcag
     4981 caggaaggtg gcaaagccgc tctggtattg gggctgtgct tcatttcgga aggtgcaatt
     5041 ccgtttgctg ctcgtgatcc gatgcgtgtg ctgccgtgct gtatcgtggg tggggcgctg
     5101 actggcgcaa tctcaatggc gattggtgcg aaactgatgg caccgcacgg tggtctgttt
     5161 gttctgctga tccctggcgc tattacgccg gtattgggtt acctggtagc aattattgcc
     5221 ggtacgctgg tggcgggttt ggcctatgcc ttcctgaaac gtccggaagt ggacgcagta
     5281 gcgaaagcag cgtaataaaa ggtgtgttac agggcagaaa tttcctgccc tgtaacattt
     5341 gatgagcgca aagagaatga ccagcttgtg aatgtaattt tatgaattta aatggtttta
     5401 tctttcagta gaaacttcaa acgttcatca aacactaagg ttatttcctc aagggcagat
     5461 tattttctgc ccttttttag tgttcctgtt ctgtgtatcc cgtcacaaat tcctcgcttt
     5521 tgtaattcag gcatttaatc gcattttgtg agcctgctca aatctgccca ttttgcctct
     5581 ctggcggtaa tttactttac gtaaaacatg catactatga gcacatgttt aaatatgaac
     5641 acatgtttaa accgtctttg cagtatctat attcttgtgg attgacaggg ttggtaattt
     5701 tttaccagag gacgtgctat gcgcgaaaag gattatgtcg taattatagg ttcggcgaat
     5761 attgatgtcg ccggatattc acatgaatca ttaaattatg tggattcaaa tccaggtaaa
     5821 ataaaattta cgcctggtgg agtagggcgc aatattgcac aaaacctggc gttgctgggt
     5881 aacaaagcct ggctactgag cgccgtaggc agtgattttt atggtcaatc gctgctaacg
     5941 caaaccaatc aatctggtgt ttatgtcgat aaatgcctga ttgtgccggg agaaaatacg
     6001 tcgagttatt tatcattact cgataatacc ggtgaaatgc tggttgctat aaatgacatg
     6061 aatattagca acgctattac agctgaatat ctcgcacagc accgtgaatt tattcagagg
     6121 gcaaaggtca ttgtcgcgga ctgtaatatc agtgaagagg cactggcatg gattctggat
     6181 aatgccgcca acgtacccgt atttgtcgat ccggtttcag cgtcgaaatg tgtcaaagtg
     6241 cgcgaccgtc taaatcagat ccacactctg aagccaaacc gccttgaagc ggaaaccctg
     6301 agtgggattg cgctgtcagg gcgtgacgat gtggcaaaag ttgctgcctg gttccatcaa
     6361 catggcctga accgactggt attgagcatg ggcggcgacg gcgtttatta cagcgatatc
     6421 agcggtgaaa atggctggtc tgcgccgatc aaaaccaatg ttattaatgt taccggagcg
     6481 ggcgatgcca tgatggcggg acttgcttcg tgttgggtag acggaatgcc gtttgccgaa
     6541 tctgttcgtt tcgcacaggg atgttcgtca atggcgctct cctgtgaata caccaataac
     6601 cccgatttat cgattgccaa cgttatatcg ttagtggaga acgcagaatg tctgaattaa
     6661 aaatttcccc tgaattatta caaatttctc cggaagtgca ggacgcttta aaaaacaaaa
     6721 aaccggttgt ggcgctggaa tcaaccatta tttctcacgg gatgccgttc ccacaaaatg
     6781 cccagatcgc aattgaagta gaagaaacta ttcgtaaaca gggcgcagta cctgccacta
     6841 tcgccattat tggcggcgtg atgaaagtgg gtttaagcaa agaaaaaatt gaattactgg
     6901 gtcgtgaagg gcataacgtg accaaagtta gtcgtcgcga tttacctttt gttgttgccg
     6961 ccggaaaaaa tggcgcaacc actgtggctt caacgatgat tattgcggcg cttgccggaa
     7021 ttaaagtatt tgccaccggg ggaattggtg gtgtgcatcg cggggcggaa cataccttcg
     7081 atatttctgc cgatttgcaa gaactggcaa atactaatgt caccgttgtt tgtgccgggg
     7141 cgaaatctat tctcgattta ggattaacca ctgagtattt agaaaccttc ggtgtgccgt
     7201 taattggcta tcagactaaa gcgctgcctg cgtttttctg ccgtaccagc tcgtttgacg
     7261 tcagcattcg tctcgacagc gccagtgaaa ttgcccgtgc aatggcggtg aaatggcaaa
     7321 gcgggctgaa cggtggcctc gtggtagcga acccgatccc ggaacagttt gcgatgccgg
     7381 aggaatctat caatgcagcc atagatcaag ccgtcgccga agccgaagag cagggcgtta
     7441 ttggtaaaga aagtacaccg ttcctgctgg cgcgcgttgc tgagctgacc ggcggtgaca
     7501 gcctgaaatc caatatccag ctggtgttca acaacgccat tctggcgagt gaaattgcca
     7561 aagaatacca gcgtctcgcg ggttaatttc tgacgggcag aaagccgtcg cccgtactca
     7621 tcaagcatac caaaacctgg cgctgacgcc gggtttactg gcatgaaggg aaaatcatta
     7681 tggatataat gagaagtgtt gtggggatgg tggtgttact ggcaatagca tttctgttgt
     7741 cagtgaataa aaagagcatc agtttgcgca cggttggagc cgcactgctg ctgcaaatcg
     7801 ctattggtgg catcatgctc tacttcccac cgggaaaatg ggcagtagaa caggcggcat
     7861 taggcgttca taaagtgatg tcttacagtg atgccggtag cgccttcatt tttggttcgc
     7921 tggttgggcc gaaaatggat gtcctgtttg acggtgcggg ttttatcttc gcctttcgcg
     7981 tacttccggc gattattttc gttactgcgc tcatcagtct gctgtactac attggcgtga
     8041 tggggctgct gattcgcatc cttggcagca ttttccagaa agccctcaac atcagcaaaa
     8101 tcgaatcttt tgttgcggtt accactattt tcctcgggca aaatgagatc ccggcgatcg
     8161 ttaaaccgtt tatcgatcgc gtgaatcgca acgagttgtt taccgcaatt tgtagcggga
     8221 tggcgtccat tgctggttcg atgatgattg gttatgccgg aatgggcgta ccaattgact
     8281 acctgttagc ggcatcgctg atggcgatcc ccggcggtat tttgtttgca cgtattctta
     8341 gcccggcaac cgagccttcg caggtcacat ttgaaaatct gtcgttcagc gaaacgccgc
     8401 caaaaagctt tatcgaagcg gcggcgagcg gtgcgatgac cgggctgaaa atcgccgctg
     8461 gtgtagcgac ggtggtaatg gcgtttgtcg caattattgc gctgatcaac ggcattatcg
     8521 gcggaattgg cggctggttt ggtttcgcca atgcctctct ggaaagtatt tttggctatg
     8581 tgctggcacc gctggcgtgg atcatgggtg tggactggag tgatgccaac cttgctggta
     8641 gcctgattgg gcagaaactg gcgattaacg aattcatcgc ttacctgagt ttctccccat
     8701 acctgcaaac gggcggcacg ctggaagtga aaaccgtggc gattatatcc tttgcgcttt
     8761 gtggttttgc taactttggt tctatcggtg ttgtcgttgg cgcattttcg gctatttcgc
     8821 caaaacgtgc tccagaaatc gcccagcttg gtttacgggc gctggcagca gcaacgcttt
     8881 ccaacctgat gagtgcgact attgccgggt tctttattgg tctggcgtaa tgctcgattt
     8941 gtagaccgga taaaacgtat taagcgttgc atccggcaat cgcgctattg caaataattt
     9001 ttactgcatc atcccggaga atttattctc cgggtccatc tccagcgcca gtcctgacaa
     9061 ctgctttctg tttttaatta gatatccttt cttgctttta attaataaac cgtcgtgaat
     9121 gaattgcgcg agaacatata aaagatgtcg ataagaaacg cctaaatact ctgccgcttg
     9181 cgtatgcttt tcgtgataaa gatcaccttc ttgtgagagt aatataaatg ctgccaggcg
     9241 attcattaac ggaaatgatt gattctgagt taaagaaaca atattacgat aatttttatg
     9301 acttaaggtg acgcaaagtt ttcgtaaaaa tagggtgtcg tttaataaca gcggacggta
     9361 atgtttcata gggagcgcaa ggcaccaaca ctcttcaata gcctgcaccg cacgcggttc
     9421 atggtctttg tcgattaact caatctcgcc aataaaacag ggagcagcaa agaaatcgat
     9481 cagcgacacg cgaccattag caagcgtggc gtaaagcctg gcgcggcctc gcgtcagata
     9541 aaacagccat gacggctgtt gcccttcctg cacaatgtag tcacgcgcta aaaaatgaaa
     9601 cagccgcgta tcggctgaaa catccgttga aaagcaatcc ttaaacgcag attcactcat
     9661 caattgctgc ttaaggtcat tattgtggat ttctttcacg atgtttatcc gctatgagat
     9721 ttctcatatt tagcatacgt acgctgttgt taagattcaa tcatcagccc ggtcgaattt
     9781 tctttgctgt agaaaaaaat aacgcaactg gaaacagagg aaataaacaa tggaaaagag
     9841 aaaaattatt ctggattgtg agccgggtca tgatgatgct attgcaatga tgatggcagc
     9901 gaaacatccg gcaatagatt tattaggcat cactattgta gcgggtaatc agacgcttga
     9961 taaaacatta attaatggcc tgaatgtttg ccagaaactg gagattaatg ttccggttta
    10021 tgcggggatg ccgcaaccca ttatgcgtaa acaaatcgtt gccgataata ttcacggtga
    10081 aaccggactg gatggcccgg tattcgagcc gctgacccgc caggcagaaa gtacccatgc
    10141 ggtgaaatat atcatcgata ccctgatggc aagcgatggc gatatcactc tggtgccggt
    10201 tggtccgctt tcaaatatcg cggtggcaat gcgtatgcaa cccgcaatcc tgcccaaaat
    10261 ccgtgaaatt gtgctaatgg ccggcgctta cggtacaggc aacttcaccc catctgccga
    10321 gttcaatatc tttgccgacc cggaagccgc ccgcgtggtg ttcacctccg gcgttccatt
    10381 agtgatgatg ggcctcgatc ttaccaacca gaccgtttgc accccggatg taattgctcg
    10441 gatggaaagg gtaggcggcc ctgccggaga gctgttcagc gacatcatga acttcactct
    10501 caaaacgcag ttcgaatact acggcctggc tggcggcccg gtgcatgacg ccacctgcat
    10561 tggttatctg atcaaccctg atggcattaa aacccaggat atgtacgtcg aagtggacgt
    10621 caacagcggc ccttgctacg ggcgtacggt ctgcgacgaa ctgggcgttc ttggcaagcc
    10681 cgctaatacc aaagtcggca tcactattga taccgactgg ttctggggct tagtcgaaga
    10741 gtgcgtgcgc ggctacatca aaacccatta agtgttgata acaagccggg acagtatcgc
    10801 aagatgcagg cccggctatt tctgatgggc agaaagccgt cgcccaaaat gacatgaaga
    10861 gataatcact atggatgtca tgagaagtgt tctgggaatg gtggtactgc tggcaattgc
    10921 gtttttactg tcagtaaaca agaagaagat cagcctgcgt accgttggcg cggcgttagt
    10981 gttacaggtc gtgattggcg ggattatgct ttggttaccg ccagggcgtt gggtcgcaga
    11041 aaaagtcgct tttggcgtcc acaaagtgat ggcgtacagc gacgcaggta gcgcatttat
    11101 cttcggttct ctggtcggac cgaaaatgga taccttattt gatggcgcag gatttatctt
    11161 tggtttcagg gtattaccgg caattatctt cgtcactgca ctggtgagta ttctctacta
    11221 catcggtgtg atggggatct taattcgcat tctcggcggt atcttccaga aagctttaaa
    11281 tatcagcaag atcgagtcat tcgtcgcggt caccaccatt ttcctcgtgc taaacgaaat
    11341 tccggcgatc gtcaaaccct ttatcgatcg tctgaatcgc aatgaattat ttacagcgat
    11401 ttgtagtggc atggcctcga ttgctggttc gacaatgatt ggttacgccg cactgggcgt
    11461 acctgtggaa tatttgctgg cggcatcgtt aatggcgatc cctggcggga tcttgtttgc
    11521 ccgcctgtta agcccggcta cggaatcttc gcaggtttct tttaataacc tctctttcac
    11581 cgaaacaccg ccaaaaagca ttattgaagc cgatgcgaca ggggcaatga ccgggctgaa
    11641 aatcgccgcc ggtgtagcga cagttgttat ggcatttgtc gccatcattg cgttaattaa
    11701 cggtattatc ggcggcgttg gcggctggtt tggttttgaa catgcctcgc tggagtccat
    11761 tttaggttac ctgctggccc cacttgcgtg ggtaatgggt gttgactgga gtgatgcaaa
    11821 tcttgccggg agtttgattg gacagaaact ggcaataaat gaatttgtcg cttatctcaa
    11881 tttctcaccc tatctgcaaa cggctggcac tctggatgct aaaaccgtgg cgattatttc
    11941 cttcgcgttg tgcggtttcg ctaactttgg ttctatcggg gtggtggtgg gggcgttttc
    12001 tgcggttgcg ccacaccgtg cgccggaaat cgcccagctt ggtttacgcg cgctggcggc
    12061 ggcgacactt tctaatctga tgagtgccac cattgccggg ttctttattg gtttagcgta
    12121 gtgtgcaatt tatgagccgg attagatgcg gtcaagcgtc gaatccggca acattgttac
    12181 accattggca ctaatgacag cgcgttatcg gcagacaggg tagggttgtt taagctgccg
    12241 ctggcgcgtg aaattgccgc gcaggccatc gcaaaacggg cgctgtcacg gaaattgttt
    12301 ccctccagaa agctatatac cagacccgcc ataaaaccat cgtcagcacc aaaactgtct
    12361 accgtcgtat gcgctggcac agtcagcaaa aattgttcac catccttttc gctgcaataa
    12421 actgactcat cgggcaaata aacaaacagt tgctgaacac cttgctgatg caatgcattc
    12481 actgcggcat tacggtcagc atcgctggtg atcgcctgtc cccataaaat ttccagctcc
    12541 ggtaaagtgg gtttcagggt gtgaatatgc gccagccagt gtttgatttt gcccgctttg
    12601 aattctgaaa cggtatcgac aaacaccggg atctcatcag cgagggtaaa gacccattcc
    12661 agcgcctcgg ctgtcaggtt gcaatcaacc agtaccacgc ccgcatgacg aagtaaatcg
    12721 cgcgacccgt tcagtagttg cggtgtcaac tgttccagca gatgggtatc gttaatcgcc
    12781 agcacggttt gatcgtctcg attggcaatt gccagatacg tcgatgtgct ttgaccatgc
    12841 aaacgaacgc agccggagac attcacgccg gcacggcgcg tttcttccag gagcatttcg
    12901 ccataaaagt catcgccaat cactgaaagc aaatggacgt cacggcctaa cagcgccaga
    12961 ttgtgggcga tattgcgtcc aacgccgcct gccgaacaat gaattgtacc gggatgagaa
    13021 gccgcttgcg ggtaatggat atccgccatc ccgcgaatat ccatattgat tgtccccacc
    13081 actacgcagt attcctgctc ggtgagaatg taacctttgc ctttaatccg gcctttgcgc
    13141 attaaatcca taatatgcgc cgcaacgcgc gaacgactga tttgcagcat gtccgcaatt
    13201 tcgttctgct gaatcagcgg gttacgccgt aaaattgcaa ggatctcctt ttcccggtta
    13261 ttcatcttca ggctaccgct ttttcagttt gttgcgcttt cagccaggcg atctcttctg
    13321 cccagatatc cgggttgatg gtttcaagga tcagcggaat gccgtcgaaa cggtcgttct
    13381 gcataatcca gcggaacgca tcatgaccga tattgccttc accgaggcta tgatggcggt
    13441 caacgcggct gccaaaggta cttttcgcat cgttaaggtg catcccgcgc agatacttaa
    13501 agccgacaat acgggcaaaa tcagcgaacg ttttttcgca ttcggctgga gtacgcaaat
    13561 catacccggc agcgaaagca tggcaggtat caatgcagac gccgacgcgg gatttatctt
    13621 ccacgccgtc gataatcgcc gcgagatgtt cgaatttaaa ccctaagtta ctgccctgac
    13681 cggcggtgtt ttcaatcacc gctgttacac cttgagtttt atccagcgca atgttgatgg
    13741 attcggcaat acgcgcaagg caatcctctt ctgaaatctg catcagatgg ctgccagggt
    13801 ggaagttgag caaagaaagc cccagtgttc gcaacgctgc atttcatcta taaaggcatc
    13861 gcgcgatttt tccagagctt cagtgaccgg atgtccgagg ttgatcagat aactgtcgtg
    13921 gggaagaatt tgcgccgatg tgtagtgata tttttcacag gcggctttga attcatcgat
    13981 ggtttgcgtc gtgagcggtg cggcacgcca ctgacgttgg tttttggtga acaaggcaaa
    14041 cgcggttgcg tcgatttcgg cggcgcgaat tgcggcattt gccagaccgc cagcagcact
    14101 aacgtgcgct ccaatgtatt tcatgcgagg actcctgtta aacccgctgg aggaaaacgg
    14161 taatgatagc gggttaacag gagcaagaag tagtggttta tgcgatgacg ctttgaatca
    14221 catagttaat cgcaccacca ccaacaatca gccaggcaaa cagtaccagt gccatcagca
    14281 gaggtttcgc cccagctttt ttcagcgcgc tgacgtgagt ggtcagaccc agcgccgcca
    14341 tcgccattgc cagcaggaag gtatccagcg ttaccagcat gttcaccacg ctctgcggta
    14401 acaggtggaa cgagttaaag atggcaacta cgatgaacaa gatggcaaac cacggaatag
    14461 tgattttgct tttctcgccg ctgttcgccc cagacagctg tttaacacgc gccgccagca
    14521 ggatgaggaa cggagccagc atcatcacgc gcagcatttt ggaaataact gctgcgtttt
    14581 ctgcatccgg gctgatggca tgacctgccg ccaccacctg cgccacttcg tgcacagtag
    14641 aaccaatgta gataccgaaa gtttccggac taaaccattg agacatcagc ggatatatcg
    14701 ccgggtagag gaaaatcgcg acggtcccga agataacaac ggttgcaaca gccacggtta
    14761 ctttactggc ttccgctttc actaccggct cagtcgccag taccgcggca gcaccacaga
    14821 tactgctacc ggcaccgatc aaccaactgg tgtgcttatc cagaccaaac actttctgcc
    14881 ccaggaagca agccagcagg aaggtactgg acagcgtcag cacgtcaatg atgatcccac
    14941 tgataccgac atcggcaatt tgcgagaacg tcagacggaa gccataaaga atgataccca
    15001 gacgtaataa atattgcttg gcaaacagca caccaccgtc acagcttttc cagatgtgcg
    15061 gatagatggt gttgcctaaa accatcccca acaagattgc gagggtgagg gcactaaacc
    15121 cggcacccgc aaccgcggga atggaaccac cccacagggc gaccccggtg ataactgcac
    15181 tcagggctaa ccccggaata aaatgccaca gtgtacgatg ttgtttctgt aaggtgatat
    15241 tcgtcataac cctctccttt aaccgggcat aaggttacga ctaactggtt taaaaataaa
    15301 attgattata tatttataat taatctttat aagtggtaag cgactatgca catcaccctc
    15361 cggcagttgg aagtttttgc agaagtattg aaaagtggat caaccaccca ggcgtcggtg
    15421 atgctggcgt tgtcgcaatc agcagtgagc gcagccttga ctgacctgga agggcagctt
    15481 ggcgttcaac tgtttgatcg cgtggggaaa agactggttg ttaatgaaca cgggcggctg
    15541 ctctatccgc gagcgttggc actgcttgaa caggcggttg aaatcgaaca actgtttcgc
    15601 gaagacaacg gcgcgattcg tatctatgcc agtagtacca tcggtaacta cattctgcct
    15661 gcagttatcg cccgttatcg ccatgattat ccgcagttgc cgattgaact tagcgttggg
    15721 aacagccagg acgtgatgca agcggtgctg gatttccgcg ttgatattgg ctttattgaa
    15781 ggaccgtgcc acagcactga aatcatttct gaaccgtggc tggaagacga gctggtggtt
    15841 ttcgccgcgc cgacttcgcc gttggctcgt ggtccggtca ccttagaaca gctggccgct
    15901 gcgccgtgga tcctgcgtga acgcggttcc ggcacgcggg agattgtcga ttatctgttg
    15961 ctgtcacatt taccgaagtt tgagatggcg atggaattag gtaactccga ggcaatcaaa
    16021 catgcggtgc gtcatgggtt gggaattagt tgcctgtcgc gacgtgtgat tgaagatcaa
    16081 ttgcaggcag gcacattaag tgaagttgcg gtccctctgc cgcgcctgat gcgtacgttg
    16141 tggcgtatac atcatcggca aaaacacctt tccaacgcgc tacggcgctt tctggactat
    16201 tgcgatcccg caaatgtgcc gcgttaagtt gctgtacaag aacatgctgg tgctgtgtcg
    16261 atttcgtgac gcagcgcctt cagcatgcat tcgccagaaa agagattggc tgctttactt
    16321 ataatccctg ggcgatcatg aaggtgtctt ataaccgtgt atttctgccg gaaggattgc
    16381 caatcgtctg ctacaatcgc gccatcattt ttaagatgga tagcattttt gtatgggttc
    16441 cgaaactaaa actacagaag cgccgggctt acgccgtgaa ttaaaggcgc gtcacctgac
    16501 gatgattgcc attggcggtt ccatcggtac aggtcttttt gttgcctctg gcgcaacgat
    16561 ttctcaggca ggtccgggcg gggcattgct ctcgtatatg ctgattggcc tgatggttta
    16621 cttcctgatg accagtctcg gtgaattggc tgcatatatg ccggtttccg gttcgtttgc
    16681 cacttacggt cagaactatg ttgaagaagg ctttggcttc gcgctgggct ggaactactg
    16741 gtacaactgg gcggtgacta ttgccgttga cctggttgca gcacagctgg tcatgagctg
    16801 gtggttcccg gatacaccgg gctggatctg gagtgcgttg ttcctcggcg ttatcttcct
    16861 gttgaactac atctctgttc gcggctttgg tgaagcggaa tactggttct cactgatcaa
    16921 agtcacgaca gttattgtct ttatcatcgt tggcgtgctg atgattatcg gtatcttcaa
    16981 aggcgcgcag cctgcgggct ggagcaactg gacaatcggc gaagcgccgt ttgctggtgg
    17041 ttttgcggcg atgatcggcg tagctatgat tgtcggcttc tctttccagg gaaccgagct
    17101 gatcggtatt gctgcaggcg agtccgaaga tccggcgaaa aacattccac gcgcggtacg
    17161 tcaggtattc tggcgaatcc tgttgttcta tgtgttagcg atcctgatta tcagcctgat
    17221 tattccgtat accgatccga gcctgctgcg taacgatgtt aaagacatca gcgtcagtcc
    17281 gttcaccctg gtgttccagc acgcgggtct gctctctgcg gcggcggtga tgaacgcagt
    17341 tattctgacg gcggtgctgt cagcaggtaa ctccggtatg tatgcgtcta ctcgtatgct
    17401 gtacaccctg gcgtgtgacg gtaaagcgcc gcgcattttc gctaaattgt cgcgtggtgg
    17461 cgtgccgcgt aatgcgctgt atgcgacgac ggtgattgcc ggtttgtgct tcctgacctc
    17521 catgtttggc aaccagacgg tatacctgtg gctgctgaac acctccggga tgacgggttt
    17581 tatcgcctgg ctggggattg ccattagcca ctatcgcttc cgtcgcggtt acgtattgca
    17641 gggacacgac attaacgatc tgccgtaccg ttcaggtttc ttcccactgg ggccgatctt
    17701 cgcattcatt ctgtgtctga ttatcacttt gggccagaac tacgaagcgt tcctgaaaga
    17761 taccattgac tggggcggcg tagcggcaac gtatattggt atcccgctgt tcctgattat
    17821 ttggttcggc tacaagctga ttaaaggaac tcacttcgta cgctacagcg aaatgaagtt
    17881 cccgcagaac gataagaaat aagttttctc ccttccttgc taagccctct caaccgagag
    17941 ggctttttca attccatttc cctgacaaat catgcggata taaaatttaa catttggatt
    18001 gataattgtt atcgtttgca ttatcgttac gacgcaatca aaaaaggctg acaaatcaga
    18061 ggctgttccg gctttctggg atgatcacct gcatacaaat aagtccaccg cgatgctgcc
    18121 gtacgcaagg ggacgtaaag aagatgtgag cgatacccat tttattttcg tagttacctc
    18181 atggagatat ggaatgttta ggttgaaccc tttcgtacgg gtcgggctgt gtttgtccgc
    18241 tatttcttgt gcatggcctg tgttagcggt cgatgatgat ggcgaaacga tggttgtcac
    18301 tgcatcttcc gtggaacaaa atcttaaaga tgcacccgcc agtatcagcg tcattaccca
    18361 ggaagacctg cagcgaaaac cggtacagaa tctgaaggat gtcctcaaag aagtgcctgg
    18421 cgtacaactg acgaacgaag gggataaccg taagggcgtt agtattcgtg gtctggacag
    18481 cagctacacc ctgattcttg tcgacggtaa acgcgttaac tcccgcaatg ccgtcttccg
    18541 ccacaatgat ttcgatctga actggatccc ggtcgattcc atcgaacgta ttgaagtggt
    18601 tcgtggcccg atgtcgtcgc tatacggctc cgatgcgctc ggcggtgtag tgaatatcat
    18661 caccaaaaaa atcggtcaga aatggtcggg taccgttacc gtcgatacca ccattcagga
    18721 acatcgcgat cgcggtgata cctataacgg tcagttcttt accagtggac cattaattga
    18781 tggtgtgctg ggaatgaaag cttacggcag cctggcaaaa cgtgaaaagg atgacccgca
    18841 aaactcaacg accaccgata ccggagaaac gccgcgtatt gaaggattct ccagccgcga
    18901 cggcaatgtc gaatttgcct ggacaccgaa tcaaaatcac gattttactg ccggatacgg
    18961 tttcgaccgt caggatcgtg attccgacta gctggacaaa aaccgcctgg aacgccagaa
    19021 ctactgcgtc agccataatg ggcgttggga ttacggcacc agcgaactga aatcctacgg
    19081 tgagaaagtc gagaacaaaa accctggcaa cagcagcccg ataacttccg aaagcaatac
    19141 ggtcgacggc aaatacacgt tgccgctgac ggcgattaat cagtttctca cggttggcgg
    19201 tgaatggcgt cacgacaaac ttagcgatgc ggtgaacctg accgggggaa ccagctccaa
    19261 aacgtctgcc agccagtacg cgctgtttgt ggaagatgaa tggcggatct tcgagccgct
    19321 ggcgctgacg accggcgtgc gtatggacga tcacgaaacc tacggtgaac actggagtcc
    19381 gcgtgcctac ctggtttata acgccaccga caccgtaacg gtgaaagggg gctgggcgac
    19441 ggcatttaaa gcaccttctc tgttgcaact tagccctgac tggacgagca attcctgccg
    19501 tggcgcatgt aagattgtgg gtagcccgga tctgaaacca gaaaccagcg aaagttggga
    19561 gctggggctt tactacatgg gtgaagaagg ctggctggaa gggggtgaat ccagcgttat
    19621 cgttttccgt aacgatgtga aagatcgtat cagcatcagc cgtacgtctg acgtcaacgc
    19681 tgcaccgggc taccaaaact ttgttggttt tgagacgggc gctaacggac ggcgcatacc
    19741 ggtatttagc tactacagcg ttaacaaagc tcgtattcag ggcgtggaaa ccgaactgaa
    19801 aattccgttc aacgatgaat ggaaactgtc gatcaactac acctacaacg atggtcgtga
    19861 tgtcagcaac ggcgaaaaca aaccgctatc cgatctgccg ttccatactg ctaacggtac
    19921 gctggactgg aaaccgctgg cgctggaaga ctggtcattc tatgtttctg gtcactatac
    19981 cgggcagaaa cgcgccgaca gcgcgacggc taaaacaccg ggcggttata ccatctggaa
    20041 taccggcgcg gcctggcagg tgactaaaga cgtcaaactg cgcgcaggcg tgctgaacct
    20101 tggcgacaag gatctcagtc gtgacgacta cagctataac gaagacggac gtcgttactt
    20161 tatggcagtg gattatcgct tctgatgaga agatgcccgg cgaaccgggc ggactttcac
    20221 ttcagtaaat actgcgcatg gaagcgcagg tgatcctcta taaaagaggc gatgaagtag
    20281 taactgtgat catatcccgg ctgaatacgc agcgtcatcg gccacgcttt ctggcgcgcg
    20341 gcttctgcca ataccgcagg ttgcaactgg tcggcaagaa actgatcatt atcgccctga
    20401 tcgataagcg tcgggatcgc atcctgcgcg ttactggcat acatcagcgc gcaactatcc
    20461 cattccagcc atgcattttt gtcctcacct aaataggtgc taaacgcttt gattccccac
    20521 gggacgctgc acggattcac aatgggcgca aaggccgaaa cgctggtgta tttacccgga
    20581 tttttcagcg ccataatcag cgcaccgtga ccacccattg agtgaccgct aatggcgcag
    20641 cggtcgctga cattaaattg cgactgaacc agcgccggta attcatcgcg cagataatca
    20701 tacatccggt aatgcgtcgc ccacggcggt tgcgtggcat taagataaaa gcctgcgccc
    20761 tggcctaaat cgtagccatc gtcgttggca accttttcgc cgcgcgggct ggtgtctggc
    20821 atcaccagta caatccccag ttccgccgct acccgctggg cacccgcctt ggtggtgaag
    20881 ttctcgtcat tgcaggttaa tccggaaagc cagtacagca ctggtggcgg ggtgtgatca
    20941 cgagggggtg ggagaaagat actgaacgtc atcgggcagt ttaaggtact ggagtcgtgt
    21001 cgccagcgtt gctgccagcc ttcaaaacag cggtgctctt cgagcatttc catgcgtggc
    21061 tccttgttgt gttgtttgca atttcttcat cataccgttt attcaatgtg ctgtgagtaa
    21121 ctttcacttc cgtatttgca taacgatgtt ttaacatctg ctgatgaaag gcagcggcaa
    21181 ttacaataat tatcgctgtg aatactggat tatgtgcgcc gcctcacgca caataatcaa
    21241 gctgtaaatc agcttaataa ctttgccccc acgcagggcg gaggcgtcac acctgcagga
    21301 gaaatcataa atgccatcac tcagtaaaga agcggccctg gttcatgaag cgttagttgc
    21361 gagaggactg gaaacaccgc tgcgcccgcc cgtgcatgaa atggataacg aaacgcgcaa
    21421 aagccttatt gctggtcata tgaccgaaat catgcagctg ctgaatctcg acctggctga
    21481 tgacagtttg atggaaacgc cgcatcgcat cgctaaaatg tatgtcgatg aaattttctc
    21541 cggtctggat tacgccaatt tcccgaaaat caccctcatt gaaaacaaaa tgaaggtcga
    21601 tgaaatggtc accgtgcgcg atatcactct gaccagtacc tgtgaacacc attttgttac
    21661 catcgatggc aaagcgacgg tggcttatat cccgaaagat tcggtgatcg gtctgtcaaa
    21721 aattaaccgt atcgtgcagt tctttgccca gcgtccgcag gtgcaggaac gtctgacgca
    21781 gcaaattctt attgcgctac aaacgctgct gggcaccaat aacgtggctg tctcgatcga
    21841 cgcggtgcat tactgcgtga aggcgcgtgg catccgcgat gcaaccagtg ccacgacaac
    21901 gacctctctt ggtggattgt tcaaatccag tcagaatacg cgccacgagt ttctgcgcgc
    21961 tgtgcgccat cacaactgat taaaaggcag gaaccatgga gcgcaacgtc acgctcgatt
    22021 ttgttcgcgg cgtcgccatt ctggggatcc tgctattaaa catcagcgcc tttgggctac
    22081 caaaggcggc ttatctcaat cccgcctggt acgatgctat tacgccgcag gatgcatgga
    22141 cctgggcatt tctcgatctc atcggccagg tgaaattcct cacgcttttt gcgctgctgt
    22201 ttggtgctgg cctgcaaatg ttgctgcccc gtggcagacg ctggatccag tcgcggttaa
    22261 cgctgttagt cttgttgggc tttattcacg gtttattgtt ctgggacggc gatattctgc
    22321 tggcttacgg gctggtgggc ttaatctgct ggtggctggt gcgcgatgcg ccatcggtaa
    22381 aaagcctgtt taataccggc gtcatgcttt atctggtggg gcttggcgtt ttgctgttat
    22441 tggggctgat ttccgacagc cagaccagcc gcgcctggac gccggatgca tcggctattt
    22501 tgtatgaaaa atactggaag cttcacggcg gcgttgaagc gatcagtaat cgtgccgatg
    22561 gtgttggcaa tagtttactg gcactgggcg cacagtatgg ctggcaactg gcagggatga
    22621 tgctcattgg tgcggcgctg atgcgcagcg gctggctgaa agggcagttc agcttacgtc
    22681 actatcgtcg tactggtttt gtgctggtgg cgattggggt gaccattaac cttcctgcca
    22741 tcgccctgca atggcagctg gactgggcgt atcgctggtg tgccttctta cttcaaatgc
    22801 cgcgggaact gagtgcgccg tttcaggcga ttggctatgc gtcgctgttt tatggcttct
    22861 ggccgcaatt gagccgcttt aagctggtgc ttgcgatcgc ctgcgtcgga cggatggcgc
    22921 tgaccaacta tctattgcaa acgctgattt gtaccacgct tttttaccac ctcggtttgt
    22981 ttatgcattt tgaccgcctg gagctgctgg cgtttgttat tccggtatgg ctggcgaata
    23041 tcctcttctc tgttatctgg ctgcgttact tccgccaggg gccggtggaa tggctctggc
    23101 gtcagttaac tttgcgtgct gccggactgg caatatctaa aacatcaaga taacgatctg
    23161 gatcacattc gttaacaaaa cggctgtaac cgtttccatt gctgtgactc gattcacgaa
    23221 gtcctgtatt cagtgctgac aaaatagccg ccagcaagca gtcatttact gcaatctcat
    23281 aacaggtagt gaatatgatc accattcgtg atgtagcgcg tcaggctggc gtctctgtgg
    23341 caacggtttc ccgggtgctc aataacagca cgctggtcag tgccgacacg cgtgaagcag
    23401 taatgaaagc cgtgagtgag ctggattatc ggccaaacgc caatgcccag gcgctggcaa
    23461 ctcaggttag cgacaccatt ggcgtggtgg tgatggacgt ttctgatgcg tttttcggcg
    23521 cgctggtaaa agcggtggat ctggtcgccc agcagcatca gaaatacgtg ctaatcggca
    23581 atagctatca tgaagcggaa aaagagcgtc acgccattga ggtgttaatt cgccagcgtt
    23641 gtaatgcgtt gattgttcac tcaaaagcat tgagtgacga tgaactggca caatttatgg
    23701 ataacattcc cggtatggtg ttaatcaacc gcgttgtgcc ggggtacgcc catcgttgcg
    23761 tttgcctgga taatctcagc ggtgcccgaa tggcgacgcg catgttgctg aataacggtc
    23821 atcaacgtat tggttatctt tcttccagcc acggcattga agatgacgcc atgcgtaaag
    23881 caggctggat gagtgcgttg aaagagcagg atattattcc gccggaaagc tggattggca
    23941 ctggtacgcc ggacatgccg ggcggtgagg cggcgatggt tgaactgctg gggcgcaatc
    24001 tacaacttac cgctgtattt gcttataacg acaatatggc cgctggcgca ctgacagcat
    24061 taaaagataa tggcattgcg attccgttac atctctcaat catcggtttc gatgatattc
    24121 ccatcgcccg ttacaccgac ccgcaattaa cgaccgtgcg ttatcccatt gcttcaatgg
    24181 ctaaattagc caccgaactg gccttgcagg gggcagcagg caatattgat cctcgtgccg
    24241 ccactgtttt atgccgacgt tagtgcgtcg ccattctgtc gcaacgcgcc agaatgcggc
    24301 ggcgatcact aactcaacaa atcaggcgat gtaaccgctt tcaatctgtg agtgatttca
    24361 cagtatctta acaatgtgat agctatgatt gcaccgtttt aatgttgtaa cccgtatgta
    24421 acagtgaata atcactttca ccgaggtaat agcgtcataa caacaattaa agccgttttc
    24481 tggagcgtta ccgggcatgg aagaacgaat tttaaaaagt gagcttcggc gttcagtaac
    24541 acttcattaa ctctactgcc ccgccgagca tttatctcaa gcactaccct gcataagaaa
    24601 aaccggagat accatgaata agaaggtgtt aaccctgtct gctgtgatgg ccagcatgtt
    24661 attcggtgcc gctgcacacg ctgctgatac tcgcattggt gtaacaatct ataagtacga
    24721 cgataacttt atgtctgtag tgcgcaaggc tattgagcaa gatgcgaaag ccgcgccaga
    24781 tgttcagctg ctgatgaatg attctcagaa tgaccagtcc aagcagaacg atcagatcga
    24841 cgtattgctg gcgaaagggg tgaaggcact ggcaatcaac ctggttgacc cggcagctgc
    24901 gggtacggtg attgagaaag cgcgtgggca aaacgtgccg gtggttttct tcaacaaaga
    24961 accgtctcgt aaggcgctgg atagctacga caaagcctac tacgttggca ctgactccaa
    25021 agagtccggc attattcagg gcgatttgat tgctaaacac tgggcggcga atcagggttg
    25081 ggatctgaac aaagacggtc agattcagtt cgtactgctg aaaggtgaac cgggccatcc
    25141 ggatgcagaa gcacgtacca cttacgtgat taaagaattg aacgataaag gcatcaaaac
    25201 tgaacagtta cagttagata ccgcaatgtg ggacaccgct caggcgaaag ataagatgga
    25261 cgcctggctg tctggcccga acgccaacaa aatcgaagtg gttatcgcca acaacgatgc
    25321 gatggcaatg ggcgcggtag aagcactgaa agcacacaac aagtccagca ttccggtgtt
    25381 tggcgtcgat gcgctgccag aagcgctggc gctggtgaaa tccggtgcac tggcgggcac
    25441 agtactgaac gatgctaaca accaggcgaa agcgaccttt gatctggcga aaaacctggc
    25501 cgatggcaaa ggtgcggctg atggcaccaa ctggaaaatc gacaacaaag tggtccgcgt
    25561 accttatgtt ggcgtagata aagacaactt ggctgaattc agcaagaaat aagactgatt
    25621 gggcgcagtc gtttactgcg cccttattgt atggccaaca aggtaaaatt atatggtcag
    25681 ctcaacgact ccgtcttccg gggaatactt gttggaaatg agcggtatca acaagtcctt
    25741 tcctggtgtt aaggcacttg ataacgttaa tttaaaagtc cggccacatt ctatccatgc
    25801 attaatgggg gaaaacggtg caggaaaatc gacattatta aaatgcctgt ttggtattta
    25861 tcaaaaagac tccggcacca ttttattcca gggtaaagag atcgatttcc attccgccaa
    25921 agaggcactg gaaaatggta tttcgatggt acaccaggag ttaaacctgg tattacaacg
    25981 ttcggtgatg gataacatgt ggctggggcg atatcccacc aaaggcatgt ttgtcgatca
    26041 ggacaaaatg taccgcgaaa ccaaagcgat ttttgatgaa ctggatattg atatcgatcc
    26101 gcgtgcgcgc gtcggcacat tatccgtttc gcaaatgcag atgatcgaaa tcgccaaagc
    26161 gttttcctat aacgcgaaaa ttgtgattat ggatgaaccg acttcttcgt taaccgaaaa
    26221 agaggtcaat catctgttca ctattattcg taaattaaaa gagcgcggct gcggtattgt
    26281 ttatatctcg cataaaatgg aagaaatctt ccagttatgt gatgaagtta ccgtattgcg
    26341 cgacggtcag tggatcgcca ccgaaccgct ggcaggactg acgatggaca agatcatcgg
    26401 catgatggtt gggcgttctc ttaatcagcg ttttcctgac aaagaaaaca agccgggcga
    26461 agtcatcctc gaggtgcgta acctaacgtc actgcgccag ccgtcgattc gcgatgtctc
    26521 gtttgatctg cataaagggg agatcctcgg tattgccggg ctggtggggg cgaaacgtac
    26581 cgatattgtt gagacgttat ttggtattcg cgagaaatcg gctggcacca ttacgttgca
    26641 cggcaaacag atcaataacc ataatgccaa cgaagccata aaccacggat ttgcactggt
    26701 aactgaggag cgccgctcaa cgggaattta tgcctatctg gatattggtt ttaactcgtt
    26761 aatttccaat attcgcaact acaaaaataa agttggttta ctggataact cgcggatgaa
    26821 aagcgatacc cagtgggtga ttgattcgat gcgggtaaaa acgccgggtc atcggacgca
    26881 aattggttcg ctctccggtg gtaatcagca aaaggtgatt attggtcgct ggctactaac
    26941 gcaaccagaa atattaatgc tcgatgaacc gacgcgcggt attgatgtcg gggcgaagtt
    27001 tgaaatttat cagttaattg ccgaactggc gaagaaaggc aaggggatta ttattatctc
    27061 ctctgaaatg cctgagttgt tagggataac agaccgtatt ctggtcatga gcaatggtct
    27121 cgtttccgga attgtcgata caaaaacaac aacgcaaaac gaaattctgc gtcttgcgtc
    27181 tttgcacctt taagattagg ggcttcccat gagtgcgtta aataagaaaa gttttcttac
    27241 ttacctgaaa gagggcggta tttacgtcgt tcttttagtt ttgctggcga ttattatttt
    27301 ccaggaccca acatttttaa gtctgttgaa cttaagtaat attctcaccc agtcatcggt
    27361 gcgtattatt atcgcgctcg gtgtggcagg gttaattgtc acccagggga ccgatctttc
    27421 tgctggtcgt caggtagggc tggcggcagt ggtggctgcg acattattgc agtccatgga
    27481 taacgccaac aaagtgttcc cggaaatggc gacgatgccg attgcgctgg ttattctgat
    27541 tgtctgtgcc attggtgcgg tgatcggttt gatcaacggt ctgattatcg cttatctcaa
    27601 cgtgacgccg ttcattacca cgctcggcac gatgatcatc gtctatggca tcaactcgct
    27661 ctattacgac tttgtcgggg cgtcgccaat ttctggtttt gacagtggct tctctacctt
    27721 tgctcagggc tttgtcgcgc tggggagttt ccgtctctct tacatcacct tctacgcgtt
    27781 gattgcggtg gcgttcgtct gggtgttgtg gaacaaaacc cgcttcggta agaacatttt
    27841 tgccattggc ggtaacccgg aagcggcaaa agtatctggt gtcaacgtcg gcctgaacct
    27901 gctgatgatc tacgcgttgt ctggcgtgtt ctatgccttt ggcgggatgt tagaagccgg
    27961 acgtatcggc tctgccacca acaacctcgg ctttatgtat gagctggatg ctatcgcggc
    28021 gtgcgtggta ggcggtgtat cgttcagcgg cggtgtgggg acggtgattg gcgtggtgac
    28081 cggggtaatt atttttaccg tcatcaacta tggcctgacg tatatcggcg taaacccata
    28141 ctggcagtac attatcaaag gggcgattat tatcttcgcc gtagcgctgg attcactgaa
    28201 atacgcgcgt aagaagtgat gtgattgcgg atattgccgg atgcgctgca tccggcaatc
    28261 ggtgccaaat gccggatgcg tctctggttt tacaacgtta ccgggtgttc tttctcgcct
    28321 ttcttaaact tcacttcccc gagctcaata caacccaccg ggcagacgtg accacaaagc
    28381 agacaaccca cacatttctc ggtattacaa tgcggtgtgc gggttttctc gctccattcc
    28441 atcgcctggt gaccgccgtc gtaacaggaa atataacagc gtccacagcc aacacatttg
    28501 tcaagattga tacggggata gacaatataa ctgcggtcta aatcttccgc cgggacaata
    28561 ttgttattcg ccagacctac catctcctgc aacgaatcaa atccctgatc ggcgagataa
    28621 tgcgataacc cgctcgccat atcttctacg atgcgatagc cgtactgcat aatgccggtg
    28681 gtgacctgta acgttgctgc gccgagcaat aaaaactccg ccgcatcttc ccaggtttca
    28741 atgccgccga taccgctgat tgggaaatcg cgcagttctg gatgggtgcg catttgctga
    28801 atgaagcgca gggcgatcgg ttttaccgct ttaccggaat atccggaaat actcgattta
    28861 ccgttaacga tcggcatacc gattttctga ttaagatcga tattggtgat ggatttaacg
    28921 gtgttaattg ccgcaatgcc atctgcgccg ccgcgcttcg ccgccagcgc cacttcgcac
    28981 atatcaccga tattcggcgt catcttcgcc agcattggca gcgtggaacc ccgtttcact
    29041 gcccgacaat atttttttac cagctccggg ctttgcccga catcgctacc catcgcatga
    29101 gaagtcattt gcggacagga gaagttacac tcgatcatat ccgcgccagc ttcttgcacc
    29161 aggcgcgcca gctcctccca ttgctgctca ttttccccca tgatcgaagc gatcaatact
    29221 ttgtccgggt aatcttcctt cagccgacgc agggcggtca gattctcttc caacggatgt
    29281 tcagcaatct gctccatatt tttgaagccg ataaaaccgg tatcttcttt caccagatga
    29341 tcaaaacgcg gcgagacttc gttggcgata aaaaagccga tcgttttaaa caccacaccg
    29401 ccccaacctg tgtcgtaggc tttggcacac atctcatagc agttgcctac cggcgaagaa
    29461 gagaggcaga acgggttggg aaagttcacg ccgcaaaaag taatcgaaag atctttcgtt
    29521 aacatgagca agctccctct aatatagtga tgaatcgcct cggcggcttc tttcccggtt
    29581 ttcacggcat agaccacggt tttgtcaccc tcaacaatat cgccagcagc aaagacttgc
    29641 ggatcgcggg tctgataatt ttgtgttttg atggtgttac gctgctgctc taactccgca
    29701 aaggcatcca gtctggcatg ctgaccgacg gcgagaataa ttttatctgc cgccatcgtc
    29761 agttcgcctg ataaccgtac atgcttaaac gtgactttat tgccttccac ggctactggc
    29821 gtgaatccat cgatgatcga aacgcccagc tcccgggcgc tggcaaactc tttttcgctt
    29881 gccggaaact catctaactc ttcacgtgct acgcaagtta ccgcctgaca gccgagaact
    29941 ttcagcgtgc tggctacgtc catcgcgaca tcaccgccgc cgataattaa tgcgctttgt
    30001 ggaatgctga tatcgccttg cggctgtcgt acacgttgca ggaagtcgac ggcaatctca
    30061 acggtcgcta tgctcaaaca gcggtagccc ggaaccgctt gataacccca cggtgaccag
    30121 taccgcgcgg ttttccgctt tcagttgctc cagggtgagt gttttaccga tttcgttatt
    30181 gcacttaatg gtcacgccca ttttttcaat acgggcgatc tctgcatcca gcactgattg
    30241 cggtaaccgg aattgcggaa taccgttacg caaccagcca ccggggtgcg cttctttctc
    30301 ataaatcgtg acgtcataac cctggtttgt cagtgtcaca ctggcctgca atccggcagg
    30361 acctgcgcca ataatcgcga ctttgccgag cgttttagta ccgggctgat aaatttccat
    30421 cccggtttgt tgttcaaaat cagtaacaaa acgctgtaag cggccgatat caatgggagc
    30481 atcaacaccg gcacgggtac aaccgctttg gcataatttc tccgtcgggc acactctggc
    30541 acaaacggca ccgagggcat tattttcgcg aattgtctcg gcagcgcctt taaaattacg
    30601 aaagtagatt gagcgaataa atttccccgg atcggtctgt gccgggcaag cctgactaca
    30661 gggagcgtcg tgacataata aacagcgaga ggcttcttta atcgccagta acggcgtaaa
    30721 agccggagtg agttcatcca gataattttg ttgcggcatg tgatcttcct taatgctatg
    30781 agtaattatg gcgaacgcta aataaatatt gcgtgctgat attaaggcta tgcgcgtggg
    30841 gaaatttaaa ctgtgaaagg attcacaaat agctgcggtt cagaatgtta tatccgcagc
    30901 ttattgagtg aagtaataat tattctcaat aattaacaga aaagagaaaa tattaacgat
    30961 agaaaataag ataaacaatc atgccgatag ataaaattcc gaagccaata tatgccacca
    31021 tataagcgaa gaagagtccg ggattttttg ctcgccagac atacaccggt tccggtgcat
    31081 ttttaacccg tttatcaatc gcgcctgcgc gccagccttt ccatgcttcg cggaaacaaa
    31141 atatcgggat aaggaaaaaa acggcaacga caaaaaactg ctgtacatcc ataactactt
    31201 tccttgtttc ttttgtaatt caagtaactg ttcgggtgta acggcgggat acccctgcgc
    31261 atcttccggc acctggtgca tagccggaat agggaccagc ggccctaaaa aacgcggttc
    31321 acgtttaaaa atataaaggt cagccagcgc accgaaacgg gcggcaaatt cgcgaatacg
    31381 tactgacagc atatctttcg gtgacggtac ggcataacac tgagcctgaa tccccatatg
    31441 cagcgcaata aataatgctc gctcacagtg gaaacgttgg gtgataataa tgaaatcatt
    31501 agtatcgaaa actttgcgtg tacgcacgat ggaatccagc gtacgaaagc ctgcgtaatc
    31561 gagaacaata tctgatgggt cgacaccagc agcgattaaa tctttgcgca tggtcatcgg
    31621 ctcgttataa ctttgcaatg cgttatcgcc gctcagtaat agataattta ccttaccgct
    31681 gttataggca ttaatcgctc cttgaatgcg gtagcgataa tactgattaa taacgccggt
    31741 acgataatat tttgctgtgc cgagcaccac gccgacctgg cggtagggga gatcctgcaa
    31801 ttcgtcgtag atataaggcg cggttttcca gctcatccag cgatcgaggc cgagcacagt
    31861 caacagcagc aagccgatca ggactaacag gctgaggaac acgcgcttta acatgcagga
    31921 gactcgagct aaggaaaatt tccatcaggc tactttaccc gctggttttg cgcaagaaac
    31981 ggcaaactgt ttgccgggaa tttcgacacc aggagagggc tggcaagccg gacaaggcgt
    32041 tcacgccgca tccggcacca ggcttaagcg agaagcacgc ggtcgatact gtgacagccg
    32101 agagctttca gcgttgcgga ggtggcatcc cactgaatca acggcgcatc ggctttttct
    32161 gccagaaccg cgcgctggat atccgggtaa tcataaccct tgagattcaa cagaattaac
    32221 gccccctgca acggcggcag agtcgggttg aatgcggcgt tttcagcgta gctgccgctg
    32281 aaaatacgac cgtctttaca ttccagcgcg acaccgcttg gtgacttact gtaaggcatg
    32341 tgcgaacggt ttgccgctgc aatcgccgcc tgagaaagcg catcaccagt cagcgcatag
    32401 ccgtgatcct gctcgtccat cagcagcgtt ttaatctcca gatctttcgg cccaaaggca
    32461 tccggcagat agtcacgcag cgcgtgtgcc tcgcggcccg gcaaatgaat acgcagatcc
    32521 agaccgctgt tcagttcatt cataaactga cggcagtgac cacaaggcgt atagttaacg
    32581 gtgatggctg caagcgcttt ttcaccactc aaccaggcgt ggctgatcgc gctttgttcg
    32641 gcatgaacgg tttgctgcat tgttgcacca ataaattcca tattggcacc aaaataccag
    32701 gttccgctca caccgcgcgc aattgcgcca acattaaaat tcgacaatgg cgtacgcgca
    32761 caggccgccg ccagcggaag tagtgcgaat gccagcgcgt cttcgtccag ccccgttgcg
    32821 ctcttcagcg atgagacttg ctccccggtc aacaaagcgg ggaagtactt gtctgccaga
    32881 ataggttcca gtgcagattg caagttatcc gcaagttggg caaaagcggt ttgaaaacgt
    32941 ggatgcatgg cgttgcctca taatcatgta atgggataac agtttacgaa cccgttatgg
    33001 ctttatatgt gatctgaatc tcattaatta tgcaacacgt gaaacttaca tcaaaaatgc
    33061 gcgacgtatc gcaaatttta gcccattact gccagaataa tcgggaaaag gaacggtgcg
    33121 atcagcgaag taattatccc gcataacacc agcgctagcg aactaaatgc accttcctga
    33181 taatccagct cggcgcagcg cgccgtaccg agggcgtgcg aggcagtccc cattgccaga
    33241 ccgcgcgcag ctttggtacg aatacgcatc gcattaagca atgtatggcc aaataccgcg
    33301 ccgaggatgc cgacgaaaat cacgcaaact gcgctgattg ccggaatacc gccaatactg
    33361 ccgccaaccg ccattgcaat aggcgtggtg actgatttcg gcaggattga cgcggcgatt
    33421 tccggtgaag cgcccatcaa caatgccacg gaagtccccg tcaccatcgc aaccacgctg
    33481 ccgataaaac agatggtgat gatcgatttc cagcgcgcgc ggatctggtg tagctgctca
    33541 tataaaggat aggccagcgc gacgaccgcc ggttgcagca gatcgtttag cacttcgcta
    33601 cctttaaagt aactgtcgta agagatgccg gtcagcatta aaaaaggaat gatcaccacc
    33661 attgctacca gcaacgggtt aagcagggga aacttatacc gtgccgccag tttgcgggcg
    33721 gcaaaaaaga caatcaacgt cagcggtaat gaccaccaga tattcgccat cattcttctg
    33781 atcctttctg acctacgact ttacgttcac cgtgtaccag ttgtgaactc cagctcacca
    33841 ccagaaaaac caccagcgta ctgacagcac aggaaaccac taccgggcca aactgcgcgc
    33901 ggagcaaatc aaaatattgc atgacgccta cgccaatcgg cacaaacaat agcgccatat
    33961 agcgaatcag tacgtagcac cccggattga cccatttcgc cggaagaatt tgcaaggcca
    34021 gcaggacaaa caggatcagc atcccgatga tgctgcccgg aatggttacc ggtagcaggg
    34081 aagcaatgaa aatgcctgca tacaggcagg cataaatcag gacgaaagcg cgtaaatatt
    34141 gccagataat gttcagtgtc ttactcataa tcaacacccc agatgagatg gattcatcat
    34201 acaattaatt cgtaaaacgt gctaccgatc acattaagaa atcagcgctt attttttcgc
    34261 tacccggtat tacgcaatga gcagcgaact caggcataca tcaagcatag agagggattg
    34321 cagtttgcag caatccctct ttgcagacct ttaatccgct gaccataggc gctaacatag
    34381 cgcctcattt ttttgcgggt gatgatatac gtgtgttact ggcaccgatg gagggagtgc
    34441 ttgactctct ggtgcgtgaa ttgctgaccg aagttaacga ctacgatctg tgcatcaccg
    34501 agtttgtccg cgtggtggat caactgctgc cggtaaaagt ctttcatcgc atttgccctg
    34561 agctacaaaa cgccagccgg acaccatctg gtacgctggt gcgcgtgcag ttgttaggtc
    34621 agttcccaca atggctggca gagaacgccg cccgtgcggt ggagttaggt tcctggggcg
    34681 tggatctcaa ttgcggctgc ccgtcgaaaa cggttaacgg tagcggcggc ggggcgacgt
    34741 tactcaaaga tcctgaactc atctaccagg gggcaaaagc gatgcgtgaa gctgtaccgg
    34801 cgcatttgcc tgtcagcgtg aaagtgcgtc tgggctggga cagcggtgag aagaaatttg
    34861 aaatcgccga tgcggttcaa caggctggcg ctacggagct ggtggtgcat gggcggacga
    34921 aagagcaggg ttaccgcgcg gagcatattg actggcaggc gattggcgag attcgccagc
    34981 ggctgaatat tccggtgatt gccaacggtg aaatctggga ctggcagagc gcgcaacaat
    35041 gcatggcgat cagcggctgc gacgcagtga tgattggtcg cggggcgctc aatattccca
    35101 acctgagccg ggtggtaaaa tataacgaac cgcgaatgcc gtggccggag gtggttgctt
    35161 tgctgcaaaa atatacccgt ctggaaaagc agggcgatac cgggttatat cacgttgcgc
    35221 ggattaaaca gtggttgagt tatttgcgta aagaatacga tgaagcaacg gaattatttc
    35281 agcatgttcg
//
LOCUS       DADMGC010000027        34275 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.027, whole genome shotgun sequence.
ACCESSION   DADMGC010000027 DADMGC010000000
VERSION     DADMGC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.027"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(94..2190)
                     /locus_tag="JNZ73_001306"
     CDS             complement(94..2190)
                     /locus_tag="JNZ73_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY8731643.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRLAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
     gene            complement(2192..2443)
                     /locus_tag="JNZ73_001307"
     CDS             complement(2192..2443)
                     /locus_tag="JNZ73_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY8731644.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            2596..3159
                     /locus_tag="JNZ73_001308"
     CDS             2596..3159
                     /locus_tag="JNZ73_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage CI repressor"
                     /protein_id="HAY8731645.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            complement(3435..3857)
                     /locus_tag="JNZ73_001309"
                     /pseudo
     CDS             complement(3435..3857)
                     /locus_tag="JNZ73_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
     gene            complement(3857..4450)
                     /locus_tag="JNZ73_001310"
     CDS             complement(3857..4450)
                     /locus_tag="JNZ73_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY8731646.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTIIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            4597..5004
                     /locus_tag="JNZ73_001311"
     CDS             4597..5004
                     /locus_tag="JNZ73_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY8731647.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            complement(5124..6116)
                     /gene="rpoS"
                     /locus_tag="JNZ73_001312"
     CDS             complement(5124..6116)
                     /gene="rpoS"
                     /locus_tag="JNZ73_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HAY8731648.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(6179..7318)
                     /gene="nlpD"
                     /locus_tag="JNZ73_001313"
     CDS             complement(6179..7318)
                     /gene="nlpD"
                     /locus_tag="JNZ73_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HAY8731649.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKIGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(7458..8084)
                     /gene="pcm"
                     /locus_tag="JNZ73_001314"
     CDS             complement(7458..8084)
                     /gene="pcm"
                     /locus_tag="JNZ73_001314"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HAY8731650.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(8078..8839)
                     /gene="surE"
                     /locus_tag="JNZ73_001315"
     CDS             complement(8078..8839)
                     /gene="surE"
                     /locus_tag="JNZ73_001315"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HAY8731651.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSSASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(8820..9869)
                     /gene="truD"
                     /locus_tag="JNZ73_001316"
     CDS             complement(8820..9869)
                     /gene="truD"
                     /locus_tag="JNZ73_001316"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HAY8731652.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICAKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(9866..10345)
                     /gene="ispF"
                     /locus_tag="JNZ73_001317"
     CDS             complement(9866..10345)
                     /gene="ispF"
                     /locus_tag="JNZ73_001317"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAY8731653.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(10345..11055)
                     /gene="ispD"
                     /locus_tag="JNZ73_001318"
     CDS             complement(10345..11055)
                     /gene="ispD"
                     /locus_tag="JNZ73_001318"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAY8731654.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(11074..11385)
                     /gene="ftsB"
                     /locus_tag="JNZ73_001319"
     CDS             complement(11074..11385)
                     /gene="ftsB"
                     /locus_tag="JNZ73_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HAY8731655.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(11579..11902)
                     /locus_tag="JNZ73_001320"
     CDS             complement(11579..11902)
                     /locus_tag="JNZ73_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HAY8731656.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(11952..12557)
                     /gene="cysC"
                     /locus_tag="JNZ73_001321"
     CDS             complement(11952..12557)
                     /gene="cysC"
                     /locus_tag="JNZ73_001321"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HAY8731657.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVAAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEEPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(12557..13984)
                     /gene="cysN"
                     /locus_tag="JNZ73_001322"
     CDS             complement(12557..13984)
                     /gene="cysN"
                     /locus_tag="JNZ73_001322"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HAY8731658.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(13986..14894)
                     /gene="cysD"
                     /locus_tag="JNZ73_001323"
     CDS             complement(13986..14894)
                     /gene="cysD"
                     /locus_tag="JNZ73_001323"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HAY8731659.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15146..16183
                     /gene="iap"
                     /locus_tag="JNZ73_001324"
     CDS             15146..16183
                     /gene="iap"
                     /locus_tag="JNZ73_001324"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HAY8731660.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(16269..17420)
                     /locus_tag="JNZ73_001325"
     CDS             complement(16269..17420)
                     /locus_tag="JNZ73_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HAY8731661.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKNRRGEKEGSDDYHLLCWHLLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     EFKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPRIEIPTFWDDDEGVELIKQLSW
                     YISATVVLADWTGSSTRFFPRVAQAIDIEDYWQKALVQAQNALTVFPPQAETAPFTGI
                     NTLFPFIENTTPLQQKVLDLDINQPGPQLFILEDVTGAGKTEAALILAHRLMAAGNAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPEVFPAPAEINRRNTVRFSRK"
     gene            complement(17614..17766)
                     /locus_tag="JNZ73_001326"
     CDS             complement(17614..17766)
                     /locus_tag="JNZ73_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY8731662.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(18030..18764)
                     /gene="cysH"
                     /locus_tag="JNZ73_001327"
     CDS             complement(18030..18764)
                     /gene="cysH"
                     /locus_tag="JNZ73_001327"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY8731663.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMVEEETRFFGLKRECGLHEG"
     gene            complement(18838..20550)
                     /gene="cysI"
                     /locus_tag="JNZ73_001328"
     CDS             complement(18838..20550)
                     /gene="cysI"
                     /locus_tag="JNZ73_001328"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HAY8731664.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(20550..22349)
                     /gene="cysJ"
                     /locus_tag="JNZ73_001329"
     CDS             complement(20550..22349)
                     /gene="cysJ"
                     /locus_tag="JNZ73_001329"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HAY8731665.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            22665..23030
                     /gene="queD"
                     /locus_tag="JNZ73_001330"
     CDS             22665..23030
                     /gene="queD"
                     /locus_tag="JNZ73_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HAY8731666.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            23108..24379
                     /locus_tag="JNZ73_001331"
     CDS             23108..24379
                     /locus_tag="JNZ73_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY8731667.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            24370..24630
                     /locus_tag="JNZ73_001332"
     CDS             24370..24630
                     /locus_tag="JNZ73_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY8731668.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GGLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            24647..25222
                     /locus_tag="JNZ73_001333"
     CDS             24647..25222
                     /locus_tag="JNZ73_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HAY8731669.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEVF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(25370..26263)
                     /locus_tag="JNZ73_001334"
     CDS             complement(25370..26263)
                     /locus_tag="JNZ73_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAY8731670.1"
                     /translation="MAGLFTPKDAAMNIAIVTINQENAAIASWLAAQDFSGCTLAHWQ
                     IEPQPVVAEQVLDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLD
                     IPTVSMRKSHWGNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPD
                     WLVSTEDLKNVSRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGTEVGYSRARVMNGG
                     VDAEKVIGISGHLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADID
                     VVDDWKVVLEALVTNIHAEYQ"
     gene            complement(26227..27006)
                     /locus_tag="JNZ73_001335"
     CDS             complement(26227..27006)
                     /locus_tag="JNZ73_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAY8731671.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTLVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRCGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(26984..28393)
                     /locus_tag="JNZ73_001336"
     CDS             complement(26984..28393)
                     /locus_tag="JNZ73_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8731672.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMSGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SIFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(28415..29869)
                     /locus_tag="JNZ73_001337"
                     /pseudo
     CDS             complement(28415..29869)
                     /locus_tag="JNZ73_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            complement(29939..30725)
                     /locus_tag="JNZ73_001338"
                     /pseudo
     CDS             complement(29939..30725)
                     /locus_tag="JNZ73_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            31044..32321
                     /locus_tag="JNZ73_001339"
     CDS             31044..32321
                     /locus_tag="JNZ73_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8731673.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            32348..33818
                     /locus_tag="JNZ73_001340"
                     /pseudo
     CDS             32348..33818
                     /locus_tag="JNZ73_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
BASE COUNT         8277 a         9068 c         8427 g         8503 t
ORIGIN      
        1 atcatcaata tcagtcatag tatttcctcg gttatttcaa atcagctttg tttcctttat
       61 ttaacagtcc gttcccgcgg actgtttttt tattcaggcc agcggatcat cttcttcctg
      121 catggcaaaa agtttttgca tccccagccg gaaatcgcgt tcaaattctt cgtcatgctc
      181 ttcttcatat tccggttgct gaactgccac cacattacca accgggcgat aaacattact
      241 cagccagggt tcagcctgtt tgtgttccag aatctgaata ccttcatctt cagcatcacg
      301 aattttttcc tcagcgcgtt tgcgcattcc tttaatacgc tgctgttgtt tgtgatattc
      361 agcactgaca gcaaaggctg cgcgtttatt gccgttccat atcgcctcac agataaagct
      421 gccgtccttg cgacgtaccg taattcgctc ggcgtcatga atgtcataac tgataagcac
      481 tttgcgaccg tgttcgtcac gcaactccgg cgcgtagtaa atattgttca gccactgaat
      541 ttcgcagcgt cttaccggtc gttccaccat tgggcggaac atgtcgcgca gctcaagatc
      601 actcagccac tcaatttcgg tgtcttcttc tgccaggcgt ttttttctga actctgtcgg
      661 gctgtaatgc cgtccgttcg gtttcatcgg cagctcagaa tgcgggcggt tgttgtacca
      721 ttccacgcct tcacgaatag cctcaataag ctccgcccac gatggcagct cgcgcattgc
      781 cgctttctgg cggtcgttca gaggcttgtc ctgttgcagg gcgttaaacg ctgaacgcag
      841 atcacggtta agtacacgca acgattcccg gtcagcaccc ttgccaaaat aggtgcgata
      901 cctcctggct atacgcatcg gcagtgtccg gttcaggcgt tcaataatgc cgcgtccctg
      961 tggattaccg gcaataccgg tagggtgttt aatccccagg cgtggcagaa tacccacgac
     1021 ctctttgtcc agcatgtcag ccgtttcacc ggaaccgtta tccgagtaat acaggaacgg
     1081 ttttccgtgg ttgcgaatgc cgtgctgtat cgcaccggca acggcgaaca cgttttcggc
     1141 aaggtcaaga ctccagccaa caacaaaccg tgttccacca tcgatcacaa acgtgacttc
     1201 cggtgagaat ggacgcccgt ggatcggatg cgcacatttc agcttcatac cgtgaccgtc
     1261 accaatccag acataattca ccggcatagc tgtccagtcg cggcgggtga atccttcaag
     1321 ctgacggtat tcactgccgg taacgcgccc tttttgtttc accacttccg gcagtttttt
     1381 catggcatac cggacaacat cataggaagg catcacctcc agcatgtacg gttcgtccgc
     1441 atggcgacgc tgccattctg cgacaaaatc ctcgtaggct tctgacatgg gtctgccgtt
     1501 cacctggcga tactgcgcca gaaattcagg cagccagctg atttcttccg gttttatttc
     1561 gtcgcgtttt cccggtgcca gtaacagcag ccgttcggcg gcgttctgcg actggttgta
     1621 tttcagaacc cactggtaaa gtggatcgcg gctgatagta cgattagcgc cacgttttgc
     1681 attggcgtta actgcggcct gttgcaggcg ctccggcagc tcacctttac gggctttttc
     1741 aacaagaaag ttaatggctt tcagtcgccc gaggttaccg gcgctttcca gcttcagcac
     1801 ttccaccacc agcgcagctc ttgcttcaga tacccagcgc tggttttcgg tcagcgcatt
     1861 caggcgttct tccatcagtt gtggagaacc gcgatatgct tcgattgcct ggacagcgtc
     1921 aggattgcgt gtttttctgg taacagcttt cacttcagaa acattgttct ctgtagccac
     1981 cagctgttcc acatagcgtt cacgcagtgc ctttcgtgtt atttcaggca gacaatcaat
     2041 gtggtactca atggcttttg tgccttcccg acggcgacta acgtcaccaa gatcacgaga
     2101 aaaacgctgt aacgcctgtc ggatgccttt tgtggtggct ggcattcctg gtacgccgag
     2161 caggtcattt acggtaacgt acgactgcat atcacgacgc cttcctgata aatccatcgt
     2221 tacgataccg gctgggccag atgatttctg gcggtacacc tatagcgtca gaaattacgc
     2281 gttcgtattt tttgtttggt gtgcgaacta ccgctttcag tgaatcctga gcataaccag
     2341 catggagtga aagctcccgg aaggaagacc acccgttatc tttgagcgcc agctgaatct
     2401 tcagcgaagg ccaatcgcgg tcactatctt ctctttgtct catctttcct ttaacctaca
     2461 aaataacccg cgcggatatc cgtatggata aagattaaga taccaaagat tccctataaa
     2521 gggttctttg gtgcttttat ggtttatttt ttaatttcat tatttatcaa tgaattagat
     2581 agaaaggaac caagaatgag aaaagcaccc caaggttctt ttcctgccga tggaaaagaa
     2641 ccgattatcg aaagaatctt taggttagta gaacgatacc cttcaagaaa tgcggctgct
     2701 cgggcatggg ggatcaatga aggcacttta aaaaattatt acaacaggcg ggattacgca
     2761 ccgataccac gcccgcatca gttaaaaaag atagcagaaa gcgaaaatgt taccttggaa
     2821 tggctcatga cgggagaggg tgatgttgaa ctaaaagatt ccaaagaacc caaaaaagag
     2881 aatgcggata atgttgatgc tcaaattcta atgttcttgt catttcttaa accacaagaa
     2941 aaacaacaag tagctgatgt tcttgggaga aaaggagcgg agcaattact cattcttctg
     3001 gatgagcaca tccaagagtt gcacgctcta gttggagttc gtagagccat agcgttaagc
     3061 cttggtaatt tgccagacga aaaggttaga gagatttacg cgttgtccga agctaagggc
     3121 gatcacttaa atctacctca gaaagcagcc agcgcataac ccagtgcaag tgcctagctg
     3181 tgctaaaatt acattggttt tggcgcttgc acccatcagc gctataataa acacctgaat
     3241 cgtttttgaa cggtctttaa aggctattgt aagaacctgt gagttatctt aaatgtttta
     3301 ttgatatcga accgtttaac atttttggac tcgctttatc ttgaaaacct tgagttccta
     3361 acttgttgat tccaatctat tcccgtcttt tttaatctct tcccggttat cttgaataaa
     3421 ttacctagtc atacgagata gaaaccgcca tcgccatcat tcaggcgaaa cagcggcaga
     3481 atatcgaaca ggttgatctc ttcgccatcg acggtgttct gcgcccaggc tggattggcg
     3541 cggcgctccg gggcaatcgg gaagttatcc cagcggcgga taaactcatc aatctgcttt
     3601 ttgaccgggg tgtttggtgg caggccaagg gaaatcgcgt ggttctgcca ggaaccgatg
     3661 gtattcatcg ccacgcgggc atcggtaaag ccgcgaatat tatcaaacca cagcgcgggc
     3721 gcgccgtcgc cgatacgccc ggtggcgtta gctgctgctg ccagatccgg ctcagcgttc
     3781 acttcttcgc tgattttcag taactggccg tggtcatcaa gcgcctgtaa aaagctgcgt
     3841 aaatcatcaa atgccattat tcattctcct gagaaaaatt ccgggcctgc ggcaatcctt
     3901 gccagcgcct ggcgtgggga tgttcgaggc caaattgatc cagcacgcgg gctaccacat
     3961 ggtggacaat gtcatctacc gtttcgggat ggttataaaa ggcaggcatc ggcggcacca
     4021 tcgccacgcc catgcgtgaa agtgcgagca tattttcgag atggatggtg ctaagcggca
     4081 tttcacgcgg caccagcacc agtttgcggc cttctttgag cacgacgtcc gccgcgcgcc
     4141 ctaccaggcc atcggcgtaa ccagcgcgga taccggcgag cgttttcata ctgcacggaa
     4201 taacgatcat gccgtcagta cgaaaagaac cggatgagat gatcgccgcc tgatccgccg
     4261 ggttatggct gaagtctgcg agggcagcaa catcgcgagc gctgtaaggc gtttctagtt
     4321 caatgatggt tttcgcccac ttcgacatca ccagatgagt ctcgacattc ggcatctcac
     4381 gcagcgcttg cagtaatgcc acaccaagag gcgcaccggt agcccctgtc atcccgacga
     4441 tcagtttcat tgcttattct cttaattgtt cgtatacgaa cattattatt aacatactcc
     4501 ttcatttcca cagcggcaag gattgaaaag taacaacatc ttcttcgtca ctaaaaaacc
     4561 gctatgatag cgacagagaa ttagcccgga gtttacatgg cgttacgaaa taaagcgttt
     4621 catcagttac gacagctttt tcagcagcac acggctcgct ggcagcacga gttacctgac
     4681 ctgaccaaac cacagtacgc ggtgatgcgc gccattgctg ataaacctgg tattgaacag
     4741 gtggcactga tagaagcagc agtcagcacc aaagcaacgc tagcagaaat gttggcaaga
     4801 atggaaaatc gtggcttagt cagacgtgaa catgacgtcg ctgacaagcg gcgacgcttt
     4861 gtctggctga ctgccgaagg agaaaagcta cttgctgcgg cgatacctat tggtgacagc
     4921 gtggatgagg aatttttggg gcgtttgagt ggagaagagc aggagctgtt tatgcagttg
     4981 gtgcgcaaga tgatgagcac atagaatgct gattgccggg taggacgctg acgtgtctta
     5041 tccaggcgac aaaaacacaa gcttcccaaa caaaagaaaa aggccagcct cgcttgagac
     5101 tggcctttct gacagatgct tacttactcg cggaacagcg cttcgatatt cagcccctgc
     5161 gtttgcagga tttcgcgcaa acggcgcagg ccttcaacct gaatctggcg aacacgttca
     5221 cgggtgaggc caatttcacg acctacatct tccagtgttg ccgcttcgta ccccagcaaa
     5281 ccgaatcgac gtgccagtac ttcacgctgt ttggcgttca gctcgaacag ccatttgacg
     5341 atgctctgct tcatatcgtc atcttgcgtg gtatcttccg gaccgttctc tttttcatcg
     5401 gccaggatgt ccagcaacgc tttttcggaa tcaccaccca gcggggtgtc taccgaggta
     5461 atgcgctcgt taagacgaag catacggctg acgtcatcaa ctggcttatc cagttgctct
     5521 gcgatctctt ccgcacttgg ttcatggtcc agcttatggg acaactcacg tgcggttcgc
     5581 aggtaaacgt tcagctcctt tacgatgtga atcggcaaac gaatagtacg ggtttggttc
     5641 ataatcgccc gttcaatcgt ctggcgaatc caccaggttg cgtatgttga gaagcggaaa
     5701 ccacgttccg ggtcaaactt ctctaccgcg cggatcagcc ccaagttgcc ctcttcgata
     5761 aggtccagca acgccagacc acgattgcca taacggcggg caatttttac caccagacgc
     5821 aagttactct cgatcatccg gcggcgagag gcgacatctc cacgcagtgc gcgacgcgca
     5881 aaataaactt cttcttcggc cgttaacagt ggtgaataac caatctcacc aaggtaaagc
     5941 tgagtcgcgt ccaacacacg ctgtgtggct ccctgcgata acagttcctc ttcggccaaa
     6001 tcgttatcac tgggttcctc ttctactaag gccttttcgt caaaaacctc aactccgttc
     6061 tcatcaaatt ccgcatcttc atttaaatca tgaactttca gcgtattctg actcataagg
     6121 tggctcctac ccgtgatccc ttgacggaac attcaagcaa aagcctggtt ccgccgattt
     6181 atcgctgcgg caaataacgc agcgggttta cggatttccc cttgtaacga atttcaaaat
     6241 gcaagcgtgt tgaactggtt ccggtgctac ccatggttgc tattttttgc cccgccttca
     6301 cttcttgttg ttcccggacc agcattgtgt cgttatgggc gtaggcactc aggtaatcat
     6361 cattatgttt gatgataatc agattaccgt agccgcgcag cgcgttacca gcataaacaa
     6421 cgcggccatc tgcggtcgcg ataattgcct gtcctttgct gcctgcgata tcaatcccct
     6481 tgttgccccc ctcagaagcg ccaaaggttt cgatcacttt gccctcagtc ggccagcgcc
     6541 aggtggagat aggcgtactg gttgatgtac tgctgacagt cggctcggtt gtgcttgctg
     6601 ttggtaccgt tacaggcgct gtgaccgtgg tcgcagttgg cttgttgttc ggcaacattt
     6661 tgttagcact ctgttcaccc gaagactcag aatacgtaat tgtcggttgc gacgcaacag
     6721 caacggtgga attttgtgca ggcttgatca caactccttg ctctgctgcg tcggcctggg
     6781 taatagcatt tccgccagtg attggcgtac cggaagcatt acccacctgc aaggtctgac
     6841 caacgttcag cgcgtatggt gcctgaatat tgttgcgctg agcaaggtca cggaaatcgt
     6901 tgccagtaat ccaggcgata tagaaaagtg tgtcgccttt tttcacggta taggtactgc
     6961 cgctataact gcctttcgga atgttcccat actgacggtt atagacgatg cgtccgtttt
     7021 ccatctgtac cggctgctga gctactggct gcactggctg gatttgcggt tgttgagtag
     7081 cctgaatttg tggctgctgt accggctgaa tttgcggttg ctgcgctgta gacgtcgtcc
     7141 ctattttcgg cggcggcgta atcaacatac cagaattagt atttgcaggc gcattgccat
     7201 taacggagct gaccggtgcc ggtggatttg aagtgtcaga acagcctgcc agccatagcg
     7261 aaaccagtga caaagccgca atgcggcgaa cggtgaattt tgggcttccc gcgctcattt
     7321 atcccccagg aaaaattggt taataaccag tgacataatt accgtgcaag gcaccctact
     7381 gaacactgga aaagatgttc acgatacgct gacctacggc aaaataacca ggaaaaatcc
     7441 aggtatttcc tcacgtttta agccagctca cccttcacta aagggacaaa gcgcacggcc
     7501 tccacggtat cgataataaa ttcgcctccc cgacgacgca cccgtttcaa atactggtgc
     7561 tcctccccta cgggtaagac gagaatcccg ccttcgtcca gctgcgtcat tagcgcagtt
     7621 ggaatttccg gcggtgccgc cgtaacaatg atagcgtcaa acggcgcacg tgcctgccaa
     7681 ccttgccatc catcaccatg acgggttgaa acattatgta aatcaagatt tttcaggcgg
     7741 cgacgtgcct gccactgcaa gcctttaatc cgttcaaccg agcaaacatg ctggacaaga
     7801 tgcgccagga ttgccgtttg atatcccgaa ccagtgccaa tttccagcac ccgcgactgc
     7861 ggcgtcagct cgagtaattc ggtcattcgc gccaccatat acggctgcga aattgtctgc
     7921 ccctgaccta tcggcaaggc gatattgtcc caggcttttt gttcaaacgc ttcatcaacg
     7981 aatttttcac gcggcacggc ggcaagtgca ttcagcacct gctcatcctg aataccttgc
     8041 gcacgtaatt gatccagaag tgcttgtacg cgtctgctta ccattgcgtg ccaactccca
     8101 cgctgtttaa ccagtctgaa accacatctt gcgcgctatg cgcagttaaa tccacatgca
     8161 gcggcgtgat ggagacatag ccctcatcta ccgcagcaaa atcggtcccc ggaccggcat
     8221 cacatttacc gcccggcggg ccaatccagt acagcgtatt gccgcgcgga tcttgctgcg
     8281 ggatcacctg atctgccgga tgtcgtgtac cgcagcgcgt cacgcgaata cctttgattt
     8341 gatccaaggg taaatccgga acgttaatat taagaatacg tccggtgcgc agcggctctt
     8401 tacacagtgc gcgcaaaatt gaacaggtta ccgccgcggc agtgtcgtaa tgtttatgcc
     8461 cgtcaagcga gacggccagc gccggaaaac ctaaatgacg gccttccatc gcggcggcta
     8521 ccgtaccgga ataaataaca tcatccccca gattcggccc ggcgttaatt ccggacacaa
     8581 caatgtccgg gcgcggacgc atcagagcat tcacgccaag atagacacaa tcggtcgggg
     8641 ttcccatttg cacagcaata tcaccatttt caaaggtaaa cgtgcgcagg gaggattcca
     8701 gtgtcagaga atttgaagcg ctgctgcggt tacgatcggg ggcgaccacc tgaacgtcag
     8761 caaactcacg caaggctttc gccagcgttt gtataccggg tgcatgtacc ccgtcatcat
     8821 tactcagcaa tatgcgcata atcacctgtt gtgttgataa gttccctgac aacgctggtt
     8881 gcaaaactac ccgccggaag ccagaaacgg atctctacgg tgacgtcatc ccaccagttc
     8941 cagcttaatt gttgcggata cagcagcatc gctctgcgcg cggcttcaac tttttcgcgc
     9001 accagtaaag cttgtaattc agtttctgcg gcgacagctg cttgttcgaa tgccagcgct
     9061 tcacgctgag ttccccattc gccactgccc ggcaatgcgg cggttatcat cagctcttta
     9121 tcgttgacgc gacgctgtaa ttccgccagt tcttcggtgg ttgctacaaa ccagctacca
     9181 cgtccggcta attgtagcgc atcgccgtca acaacttgat taacgtctgc ttttttgagg
     9241 cgctcagcaa caatctgatt aaacaacgca ctgcgggctg ccgacaacca aaaactccgt
     9301 ttattgcgat cgcgcaccgg agtattggtt tgcgcccagc gcagcgcgcc ctgcaagttg
     9361 ctaccgccaa tcccaaaacg ttgggcaccg aagtagttcg gtacaccttt tgcgcaaata
     9421 tcgatcagac gttgttcaac gtcatcgcga ttgctcactt cgcgcagaac cagggtaaag
     9481 gcgttacctt tcagcgcccc taaacgcagc ttgcgcttgt gccgcgcata ctccagcacc
     9541 tggcagcctt ccagttgaaa ggcgctcaga tcgggcattt ccttgcccgg cacgcgagca
     9601 cataaccact gttccgttac agcatgtttg tctttttgcc cagcgaagct gacttcacgg
     9661 gaatgaattt tcaggaattt cgccagtgca tccgccacaa aacgggtatt gcagccgttt
     9721 ttgaggattc taaccagaat atgctcacct tcaccatcag gctcaaagcc caaatcttcc
     9781 accaccacaa agtcttccgg attggctttc agcagcccgg tgccttgcgg tttaccgtgg
     9841 aggtaagtga gattatcaaa ctcaatcatt ttgttgcctt aatgagtagc gccaccgctt
     9901 cacaggcaat cccttcccca cgtccggtaa atccaagttt ttccgtagta gtggctttca
     9961 cgttaacatc atccatatgg cagccgagat cttcggcaat aaacacgcgc atttgtggaa
    10021 tgtgcggcaa catcttcggt gcctgagcga tgatagtgac atcgacgttg ccaagggtat
    10081 aacccttcgc ctgaatacga cgccaggctt cgcgtagcag ctcgcggctg tcggcacctt
    10141 taaatgccgg atcggtatcc gggaacagtt tgccgatatc ccccagcgcc gccgcgccaa
    10201 gcaatgcatc ggtcaacgca tgaagcgcca cgtcgccatc agaatgcgcc agcaatcctt
    10261 tttcgtaagg aatgcgtacg ccaccaatga taattgggcc ttcaccgcca aaggcgtgta
    10321 cgtcaaaacc gtgtccaatt cgcattatgt attctcctga tggatggttc gggtgaggta
    10381 aaactcggcc agtgccaaat cttccgggcg cgtgacttta atgttatccg cgcggccttc
    10441 gaccaactgc ggatgaaatc cgcaatattc cagcgccgag gcttcgtcgg taatggtagc
    10501 accttcattt agagcgcgcg tcagacagtc atgtaacagc tcacgaggga aaaattgcgg
    10561 cgtcagcgcg tgccataagc cgttgcgatc aacggtatga gcaatggcat ttttgcccgg
    10621 ttcggcacgt ttcatagtat cgcgcactgg tgcggcgagg atccctcccg tgcggctggt
    10681 ttcgctcaac gccaacaatc gcgcgaggtc atcctgatgc aaacaaggac gagcggcgtc
    10741 atgcaccaat acccactgca catcgccagc ggctttcaga cctgccagca cggaatcggc
    10801 acgctcatca ccgccatcta caacggtgat ttgcggatga ttcgccagag gaagttgtgc
    10861 aaaacggcta tcgccaggac ttatggcaat gacgacacgt gtcacccggg gatgcgccag
    10921 cagcgcatgc accgagtgtt caagaatggt ttgattaccg attgagagat attgcttagg
    10981 acattccgtt tgcattcgac ggccaaatcc ggccgccgga accacggcgc aaacatccaa
    11041 atgagtggtt gccatgttaa ttcccgggct gatttatcga ttgttttgcc ccgcagactg
    11101 tgcgcgcttc gacgcgtcag gcaccagacg ataaaaagtt tcgcccggcc tggtcatgct
    11161 gagttcatta cgcgcacgct cttcgagcgc ctcctggccg ccattgagat cgtcaatttc
    11221 ggcaaaaagt tgatcgtttc gcgctttaag tttcgcgttt gtagcttgca gtgccgccac
    11281 atcatcattg acgcgggtat agtcatgtat accgttctta ccgaaccaca gcgaatactg
    11341 tagccagacc agaatagcca gcaacagcag cgttagttta cccatcctgc cccctgaaaa
    11401 actgcatcat catcccatgc atccgaagac gactctacat cctctgttgg ggataccgcg
    11461 acaacgcggg caaatgtacc acatttgtcc attgttacgt atacccaggg cgtgcagaac
    11521 ataatctcat tattagttac ggtttgaatt atgaacagag gagacaagaa agtacaaatt
    11581 agcccagtag ccacataaac agtgcgccaa acataatgcc tactgtcacc agggtgaaaa
    11641 caatactgta gcgtagcttt ccgtccatca atgaatgcag cgcaatcccc accactaccg
    11701 cgacaggcat cagtgccaga aagaaaggcc aggtgtagat aaagaagaac agcgtgttag
    11761 agccataaat caacatcggc atcgccagcg caaataacca ggagataaaa ccgaccacgg
    11821 caccaggcag tgaccatgta gtttcttcat cctcagtaag gctgtcgtta tttgttagtg
    11881 taatgttatg gctattacgc atatttgatc ctgttacttt gacgaaccgg gcattgaaac
    11941 ccggtggtgt ctcaggatct gataatatcg ttctgtctca acagatctaa taattgctgt
    12001 accaaatttg ttactaattg ttcaccattg agatgaattt ctgccgattc aggctcttcg
    12061 taaacggaat ctattcccgt aaagttgcgc agttcaccgg cacgcgcttt cttatataag
    12121 ccttttggat cgcgggcttc gcaaatcgcc agcggcgtat cgacaaacac ttcaataaag
    12181 cgcccttctc ctacgcgttc gcgaaccatc tggcgttcgg cgcggtgtgg cgagataaat
    12241 gcggtcagca ccaccagtcc ggctgcaacc atcaaattcg ccacttcacc gacgcgacgg
    12301 atattctctt tacgatcggc atcgctaaaa ccgagatcgc tgcataatcc gtggcgaaca
    12361 ttgtcgccat ccagcagata cgtactgacg ccgagtttat gtaacgcctc ctccagcgcc
    12421 ccggcgaccg ttgatttacc ggacccggag aggccggtaa accacagcac tacaccacga
    12481 tgaccgtggt gtagttcgcg ttgttgcaca gtgaccggat gactatgcca gacgacgttt
    12541 tcgtcatgca gcgccattat ttatccccca gcaaatcgcg cgcgccccag tgcggaaagt
    12601 ggcgacgaac cagggcattc aattccagtt cgaatgcact gaattcagat gacgcagctg
    12661 ttgcctggct aactggctcg tgcaccatac cggcacccac ggtcacattg ctcaggcgat
    12721 cgataaaaat cagcccaccg gtaaccggat tttgctgata gcgatctaac accagtggct
    12781 cgtcaaaagt gagatccacc aggccgatgc cgttcagcgg caggttttca acttcgcgtt
    12841 gggtaaggtt attaatatca acctgataac gaatgccatc aacacgagca cgcgtcttct
    12901 taccggcaat tttgatgtca taactctggc ccggggaaag cggctgttcc gccatccata
    12961 ccacatccac cgacgcactc tgcaccgccg gtaacgcttc gtctgccgcc agcagcagat
    13021 caccacggct gatatcgatc tcatccgtca gcaccagggt gatcgcttct ccggcaaaga
    13081 cttcttcgcg atcaccatca aaagtcacga tccgcgcgac gtttgattcc acaccagagg
    13141 gcagcacttt tacacgttgc ccgacttcca cgcgaccgga tgccagcatt ccggcgtagc
    13201 cacgaaaatc gagatttggg cggttaacgt actgcaccgg gaagcgcatt ggctgggcat
    13261 ccaccactcg ctggatctcc acggtttcca gcacttcgag cagtgtcaga ccgctgtacc
    13321 acggcatact ttcactttgc gaagccacgt tgtcaccttc cagtgcggag agcggcacaa
    13381 agcggatatc cagattaccc ggcagctgcc cggcaaaggt cagataatct tcacgaatac
    13441 gggtgaacgt ctcttcactg taatccacca gatccatttt gttgatcgcc acgaccagat
    13501 gtttgatccc caacagtgtg gagataaaac tgtgacgacg ggtttgatcg agcacgcctt
    13561 tacgggcatc aatcagtaag atcgccagtt cacatgtcga tgcgccagtc gccatattgc
    13621 gagtgtactg ctcgtgccct ggggtgtcgg cgataataaa tttacgcttc tcggtagaga
    13681 aatagcggta ggccacatca atagtgatgc cctgttcgcg ctcagcttgc aggccgtcca
    13741 ccagcagagc cagatccagc ttttcgccct gggtgccgtg acgcttactg tcgttatgca
    13801 gcgatgagag ctgatcttcg tagatttggc gggtatcgtg cagcagacga ccaatcaggg
    13861 tacttttgcc gtcatcgacg ctaccacagg tcagaaaacg cagcaggctt ttatgttgtt
    13921 gcgcaatcat ccaggcttcg acgccgcctt cattggcgat ttgttgtgca agtacggtgt
    13981 tcatcttaaa aatacccctg acgttttttc agctccatgg atcccgcctg gtcgcggtca
    14041 atcacgcgac cctgacgttc actggtggtg gaaaccagca tctcttcgat gatttccggc
    14101 agtgtttgtg catttgactc caccgcaccg gtcagcggcc agcagcccag cgtacggaaa
    14161 cgcaccatcc gttttttgat cacttcgccc ggttgcaggt cgatacggtt gtcatcaatc
    14221 atcatcaaca taccgtcgcg ttccagaacc ggacgttccg cagcgagata cagcggcaca
    14281 atgtcgatat tttccagcca gatgtattgc cagatatcct gctcggtcca gttagagagc
    14341 gggaagacgc ggatgctttc gcctttgtta atttgcccgt tgtagttgtg ccacagctcc
    14401 gggcgctgat ttttcggatc ccagcgatgg aagcggtcac ggaaagagta gattcgctct
    14461 ttagcgcggg atttctcttc gtcacggcgc gcaccaccga aggcggcatc aaaaccgtat
    14521 ttgttcagcg cctgtttcag gccttcagtt ttcataatat cggtatgttt cgcgctgccg
    14581 tgcacgaatg gattaatccc catcgccacg ccttccgggt ttttatgcac cagcagctcg
    14641 cagccgtagg ctttcgccgt acgatcgcgg aactcataca tctcgcggaa tttccagccg
    14701 gtatcgacat gcagcaacgg gaaaggcagc gtacctggat aaaacgcctt gcgcgccaga
    14761 tgcagcatga cgctggaatc tttaccgata gagtagagca tcaccggatt tgagaattct
    14821 gccgccacct cgcgaataat gtggatgctt tccgcctcca gttgccgcag gtgagtaagt
    14881 cgtttttgat ccataaccgt tcctttgcaa taccgctatt ttcttgccat cagatgtttc
    14941 gactataggg agcgtaagag aacgaatgaa attaccaatt agaatgagta gttccttaac
    15001 ggaataacga tttggcaaag ctaatatcaa aaagtgctta aggcaccgga tttcgggcgt
    15061 ttaggaagat ttgaaattgt tttagcgcag cggcagtttc atactatggc ggtaaaaaaa
    15121 tttgcatggt atttaaggac tcactatgtt ttccgcattg cgccaccgta ccgctgccct
    15181 ggcgctcggc gtatgcttta ttctccccgt acacgcctcg tcacctaaac ctggcgattt
    15241 tgctaatacc caggcacgac atattgctac tttctttccg ggacgcatga ccggaactcc
    15301 agcagaaatg ttatctgccg attatattcg ccaacagttt cagcaaatgg gttatcgcag
    15361 tgatattcgg acatttaata gtcggtatat ttataccgcc cgcgataatc gcaagaactg
    15421 gcataacgtg acaggaagta cggtgattgc cgctcatgaa ggcaaagcgc cgcagcagat
    15481 catcattatg gcgcatctgg atacttacgc cccgctgagc gatgctgacg cagatgccaa
    15541 tctcggcgga ctgacgttac aaggaataga tgataacgcc gcaggtttag gtgtcatgct
    15601 ggaactggca gaacgcctga aaaatacgcc taccgagtat ggtattcgat ttgtggcgac
    15661 cagcggcgaa gaggaaggaa aattaggcgc tgagaattta ctcaagcgga tgagtgacac
    15721 cgaaaagaaa aatacgctgc tggtgattaa tctcgataac ttaattgttg gcgataaatt
    15781 gtatttcaac agcggtgtaa aaacccctga agcagtaagg aaattaacgc gcgacagggc
    15841 gctggcaatt gcacgcagtc acggaatagc cgcaacgacc aatccgggtt tgaataaaaa
    15901 ttatccgaaa ggcactgggt gttgtaatga cgcagaaata ttcgacaaag cgggcattgc
    15961 tgtactttcg gtggaagcga ctaactggaa tcttggaaat aaggatggtt atcagcaacg
    16021 cgcaaaaaca cctgccttcc cggcgggaaa tagctggcat gacgtaagac tggataatca
    16081 acaacatatt gataaggcac ttccaggaag aatagaacgt cgctgccgtg acgttatgcg
    16141 gataatgcta ccgttggtga aggagctggc gaaggcgtct tgatgggttt gaaaatggga
    16201 gctgggagtt ctaccgcagg ggcggggaat tctaagtgat acccatcatc gcatccagtg
    16261 cgtccggttt acttccggga aaaccgcacc gtattgcgcc ggtttatctc cgctggagcg
    16321 gggaacacct ctggcgaata gaaagcaagc caggttttga ccagtcgatc gtacatggca
    16381 ttggccgttg ccattgttgg caggccaaaa aacagcccct gagcgttacc cgcagccatc
    16441 aacctgtgcg ccaggataag cgccgcttct gttttacctg cgccggtcac atcttccaga
    16501 ataaacaact gtggtcctgg ctgattgata tccagatcca gcaccttttg ctgtaacggt
    16561 gtcgtattct caataaaagg aaacagcgta ttaattccgg tgaaaggcgc ggtttctgct
    16621 tgtggcggaa agacggttaa tgcgttttga gcctgaacta aagccttttg ccagtaatct
    16681 tcaatatcta ttgcctgtgc gacgcgtgga aaaaatcgcg ttgacgaccc tgtccagtct
    16741 gcaagtacga cggttgcaga gatataccag gaaagttgtt ttataagttc aacgccctcg
    16801 tcatcatccc agaatgtggg gatctctatt cgcggaaaca gtggcttgat ttcaaggaga
    16861 aaatctcgtg ctgcagcttt gtcttcaggc agaaaattat ccaattcatc catacgatca
    16921 gggggccgac catgatgccc tgtcgtcacg ggcatccaca tttctatcac tcgcctaagt
    16981 ttgcgcggag agagcgaaga tgaaggaagt aactcctgac attcacttaa ataatgattc
    17041 cacaaccagt agcccagcgt tgaatgagag attttttcgt aattttttct ggcaccttcc
    17101 tgaatcttga attcaggggg caggtaaagt tgttgaaaag agcgggcaaa ttttccaata
    17161 tcgtgccagc acagcaacca ggcgaaaaat tgagctgcct gttccttgtc agaaatccct
    17221 aattgacgaa aataatcagc cagcccgaag caatttcttt taaccattaa atagcccatt
    17281 gcggccacat ccagcaaatg ccagcaaaga aggtgatagt cgtcgctacc ctctttctcg
    17341 ccacgtcggt tttttcccca gaaatcaaag aaagtcacaa tatttttatc cttcagtaaa
    17401 cttaaaggat atttacgcat attttaaaaa ctatctgtga tatatatcag ccaataaaac
    17461 atttacatta tgaataaatt aatgattttc atttgaaatt cataataata aatagagact
    17521 atatattcac ataaaaaata taaattttaa aaaagggtgc aggagaccaa accatcacaa
    17581 ttcagtacgc ggggttttac ccccgcgtgg tggctacttc ttcgattcgt aagcgagaac
    17641 tgccttaaac tccataccac gttctctgat actcaactca cataacgagt cacctaccat
    17701 caacgtgaat cccagtactg tacaacacag tacgatgatt gccacaaggg catattttgt
    17761 cagcatgtct tgcctccctg caaagaagag actaacatcc gaattgctac gttcgaacgc
    17821 gagcctcagg ttgatagaaa tatcgcctgg ggcttttgtc catctggaac ctcgcgaatg
    17881 cttaacgcca gacagcctca agcacccaac gccattctat acctgttaat tttccctgcg
    17941 atgttcacct ttaaatgccg gaatgctgct cgcaaaataa aaacgccccc atcatttctg
    18001 acagaggcgt ttaatttatc cggcaatatt tacccttcgt gtaatccaca ttcccttttt
    18061 aagccaaaga aacgcgtttc ttcttccacc atgccgggtt cccatttacg ggttgtatgg
    18121 gtatcgccca ccgataaata tccttcgtcc cataatgggt gatatttcag gccatgtttt
    18181 tgcaggtact gataaatagt tcggttatcc cagtcgataa tcggcagcac tttaaatacg
    18241 ccacgctgaa ttgccagcac cggtaaattg gcacggctgc cggattgttc acgccgcagg
    18301 ccagcaaacc acgtttgcgc attcagttct ttcagcgccc ggttcatcgg ttcgactttg
    18361 ttgatgtcat tgtacttttc aatgccttca acgccctgtt cccacagttt gccgtagcgt
    18421 gcttcctgcc aggcggcgct ttcggtagcg cggtacactt tcaggttgag cttgagtttg
    18481 tccgttaact cgtcaataaa gcggtaggtt tccgggaaca gatagccggt gtcggtgagg
    18541 atcaccggaa tatccgggtg aatctgattc accagatgca ggctcaccgc cgcctgaatg
    18601 ccaaagctgg aagaaagtac atattcaccg ggcagattat ccagcgccca ggcaacgcgg
    18661 ccttcagcgt ccagtttttc cagttcggcg ttagtttctg ccagcgccag aatgcgatct
    18721 accttcggca gttcgttcag ggcgtttaga tcgagtttgg acatagattc ctcactgttt
    18781 gccttgcctg atgcgacgct cacgcgtctt atcaggccta caagaccggg ctgatggtta
    18841 atcccacaaa tcgcgcgccg gatcgagcac cgggcgaatg atgcccgcac gcaccgtaaa
    18901 gtcgccgaag ccttcacccg cttcgcgctc tttcgcccag cgccctatca gttcatcaag
    18961 cgacgccagg atttccggct cggtgatgtt ttctttatac atccgtggga tacgtgtccc
    19021 aatgcggttg ccgccaagat gcaggttgta gcgacccggt gctttaccca ccaggcccac
    19081 ttccgccagc atcgcgcgac cacaaccgtt cgggcagcct gttacacgca tcacgatatg
    19141 ctcatcgctg acaccatgtt tcgccattaa attatcgatg ttgtcgataa aagacggcag
    19201 gaaacgctcc gcttccgcca tcgccagcgg gcaagtcggg aatgacacgc aggccatcga
    19261 gttttcacgc tgcggcgtga cggcattcat taacccgctc tctttggcaa ttttctcgat
    19321 cttcgctttc tcgctttccg gcacaccggc gatgatcaga ttctggttcg ccgtaatgcg
    19381 gaaatcacct ttgtggatct tcgcgatctc cagcaggcca gttttcagcg gacgccccgg
    19441 ataatcaagg atgcgaccat tttcgataaa cagcgtcagg tgccagttat catcaatgcc
    19501 cttaacccag ccaatacgat cgccgcgtcc tgtaaattca tacgggcgga tcggttcaaa
    19561 tttgatcccc gcgcgacgct ccacttccgc tttaaacgtc tcaaccccca cgcgctccag
    19621 tgtgtatttt gttttggcat ttttacgatc ggttcggtta ccccagtcac gctgagttgt
    19681 cacgacggct tccgccaccg ctagcgtatg ctctagcggc agatagccaa actcactcgc
    19741 cgtgcgggcg taggttttct tgttaccgtg ttcgatagaa agcccaccgc ccaccagcag
    19801 gttgaagccc accagcttgc cgttttccgc gatcgccacg aagttcatgt cgttggcgtg
    19861 cagatcaata tcgttctgcg gcgggatcac taccgtggtt ttgaacttac gcggcaggta
    19921 ggtctgacca aggatcggtt cttcatcggt ggtggcgact ttttcctgat cgagccagat
    19981 ctccgcatac gcgcgggtac gcggcagcag atgttcagaa atcttcttcg cccactcgta
    20041 cgcttctgcg tgcagctgcg actcgtaagg gttcgaggtg cagagtacgt tacggttcat
    20101 gtcgttagca gtcgccagcg catcaagacc gaccgagtgc agcatctggt gcaccggttt
    20161 gacgttcttt ttcagaatgc cgtggaactg aaacgtctgg cggttggtca ggcgaatgct
    20221 gccatagatg gtgttttcac cggcaaattt atcgatcgcc tgccactgtt tggtggtgat
    20281 aactccaccc ggcagacggc agcgcaacag catcgcgtgg cgcggctcca gcttctgttc
    20341 agcacgttcg gcgcggatgt cgcggtcatc ctgctgatac atgccgtgga agcggatcag
    20401 caggaagtta tcgcccttaa agccgccggt cagaccgtcg tttaaatctt ccgcaatggt
    20461 gccgcgcagg tagttgcttt caagcttcat gcgctcggcg tctgtcagtt ttccttcgac
    20521 cactaaaggc cctggatgtt tttcgctcat tagtagacat ctcgctgata acggcgctct
    20581 acgcgcagct cacttaaaaa ttcatccgcc gcttcggtgt ccatgccacc aaattcggca
    20641 atcacttcca gaagtgcctg ctcaacgtct ttcgccatgc gattagcgtc gccgcagaca
    20701 taaatgtggg caccgtcatt gatccagcgc cacagctccg cgccctgttc gcgcagttta
    20761 tcttgtacgt aaactttttc tttttggtcg cgcgaccagg cgagatcgat ccgcgtcagc
    20821 acaccctctt tgacgtagcg ctgccactcc acctgataca ggaagtcttc cgtaaaatgc
    20881 ggattgccaa agaacagcca gtttttacct ggcgcttcgt cagcggcgcg ttgctgcata
    20941 aaggcgcgga acggcgcaat accggtgcct gggccaatca taatcaccgg ggtttccagg
    21001 ttagcgggca ggcggaagtt atcgttatgt tcgataaata cgcggacttc gccctcttct
    21061 tccacgcggt cagcgaggaa gctggaggca ccaccggcac gggcgcggcc ttccacgtcg
    21121 taacgcacca caccaacggt gacgtgtact tcgttctcga cttccgcctg cgaggaggca
    21181 atggaataga gacgcggggt cagcgggcgc agcagattaa ttagcgcttc ggcatcaagc
    21241 tgtgccgggg agaaacgcac catgtcaaca atcagcgtcg tcgcggcgta atgctgtaac
    21301 ttcgctttat cgcccaccag cggcagcagt gtttcactgc gggtaagcgt ggcgtaattc
    21361 tcaacaatgt tggcggtgtt gacggtcagt tcgaagtgcc actgtagcgc ttcgttcaga
    21421 ggcaacgttt tgccctcgac agtgacaggt tcatcgcctg tcagccacag cagttcgaca
    21481 agttctttca ccagtgccgg atcgttctga taccagacgc ccagcgcgtc acccggctgg
    21541 taacgcaggc ccgagtcgcc taagtcaatt tcgatatggc gaacgtcttt ttcagagtta
    21601 cgcccagtaa ttttctggtt aacagagagg ctagccacca gcggcgcgtc tttgctgtac
    21661 gggctggtgt ggatttcatt taccgcgcca gtagcgacgg attgcgaagg tgccgcgaca
    21721 ggcgcacgcg atttaagcgc atcaaccacg cgggcgcgcc actcgctggc agcagcctgg
    21781 tattcaacat cagaatcgac acggtcgagc aggcgttcac cacccagttc cgccagcttg
    21841 ctgtcaaaat ctttcccgga ctggcagaaa aattcataag agctatcgcc gaggctaaac
    21901 acggcaaacg cggtgttttc cagctttggc gcttttttgg agaacaggaa cttatgcagc
    21961 gcgacggctt cttccggcgg ttccccttcc ccttgcgttg acgtcactac gatgagcagt
    22021 ttttcgctgg cgatttgttt gaatttatag tcgcccgcgt tcaccagctt aacgttcagt
    22081 tttgctgcta ataaatcatc gcgtaatgct tcagcaaccc ggcgcgcatt gccggtttgc
    22141 gaagcagaga taatcgttat acccggcatt tctgcggcgg gcgctggcgt cactgcaagc
    22201 gcaacaggct gctggttgag cacgccccag aaatagccag aaacccaggc aagctgggta
    22261 ggcgttaaat cggtcgtggc cgcctgaagg cgtgccagtt gctccgggtt caacggaagc
    22321 aacgcggaag gtgggacctg tgtcgtcatg cgtcgttatg ttccagtaag caaagctgtt
    22381 tctgcgccct gtcagcgccc ataaaacaga agagaaggta aggttaacgg ggtgaacggt
    22441 gtggattaaa gacgggatag cgataactaa taaccaaatc gactaacctg ttttagcaat
    22501 agtctttaac aacaaaatag attaaccaac ctaatgaaaa acaaatgaat ttagccaatc
    22561 attaagataa atcagcgatt ttgcgcaaca aagtcgtttt agataatgcg aaaaaacagc
    22621 ctttccggta ctctacggcg gttttatcgt cctgtagaga aattatgatg tccaccacgt
    22681 tatttaaaga tttcaccttc gaagccgctc accgcttacc acatgtcccg gaagggcata
    22741 aatgtggtcg cctgcacggg cattccttta tggtgcgact ggaaattacc ggggaagtcg
    22801 atccgcatac gggctggatt atcgatttcg ctgaactgaa agcggcgttt aagccaactt
    22861 atgagcgcct agatcaccat tatctcaatg atattccagg tctggaaaac ccaaccagcg
    22921 aagttttagc aaaatggatt tgggatcagg ttaaacccgt tgtgccgctg ttaagtgcgg
    22981 tgatggtaaa agaaacctgc accgcagggt gtatctatcg cggcgaatga taagagtgtg
    23041 tcggcggtca atttcccttt agtaacgcta tgttagggtg ttgtgttctg gatatctggg
    23101 gcatgacatg gaagacgact gcgacattat tattattggt gccggtattg caggcaccgc
    23161 ttgcgcgtta cgctgcgcgc gagcggattt atccgttttg ttactcgaac gcgctgaaat
    23221 ccccggcagc aaaaatcttt ccggcaggcg gttatatacc catgcactcg cggaactcct
    23281 cccgcaattt catctgaccg cgcctcttga acgacgcatc actcacgaaa gcctttccct
    23341 gttaacgccg gatggcgcaa cgacgttttc cagcttacag cccggcggtg aatcctggag
    23401 tgtattacgt gcacggttcg atccgtggct ggttgccgaa gccgaaaaag aaggtgtcga
    23461 atgcatcccc ggagcgacgg tggatgcact gtatgaagaa aacggcagag tctgtggcgt
    23521 tatttgtggt gacgatattc tccgcgcccg ttatgtggtg ctggcagaag gtgccaacag
    23581 cgtcctggct gaacgtcacg ggttagtgac tcgtcctgct ggcgaagcga tggcgttggg
    23641 gatcaaagaa gtgctgtcgc tggaaacatc cgctattgaa gaacgttttc atctggagaa
    23701 taacgaaggc gcagcgttgc tgttcagcgg cggaatctgt gatgacttac ccggcggcgc
    23761 atttctttat actaatcaac aaacgctctc gttagggatt gtttgcccgc tctcttccct
    23821 tacgcaaagt cgtgttccgg caagcgagct gctgactcgc tttaaagcgc atccggcagt
    23881 gcgcacgctt atcaaaaaca cggaatcact ggagtatggt gcgcatctgg tgccagaagg
    23941 tggcttgcac agtatgccgg tgcaatacgc cggtaacggc tggctgctgg tgggcgatgc
    24001 gttgcgcagt tgcgtcaata ccggaatttc cgtgcgcggc atggatatgg cgctgactgg
    24061 cgcacaggcg gcggcacaaa cgctaataag cgcctgccag caccgcgagc cgcaaaatct
    24121 gtttgcgctt tatcatcaca acgtagagcg cagcctgcta tgggatgttc tacaacgtta
    24181 tcagcatgtt ccggcgcttt tgcaacgccc tggctggtat cgggcgtggc ctgcgttaat
    24241 gcaggatatt tcccgcgatt tatgggatca gggtgataaa cctgttccac cgctgcgcca
    24301 gttattctgg cgtcatttac gtcgtcatgg cctgtggcat ctggcgggcg atgttatcag
    24361 gagtctgcga tgtctgtagc ccgtaatctc tggcgcgttg ctgatgcgcc gcacattgtt
    24421 ccggctgact ccgttgagcg tcagatggta caacggttga ttaacgcctg tccggtaggt
    24481 cttttttctc tcacacagga aggtggctta cgtattgact atcgcggctg cctggagtgt
    24541 ggcacctgcc gtttgctgtg cgacgaatcc acactacaac agtggcgcta tccgccttcc
    24601 ggattcggca tcacctaccg ctttggataa ataataagga taatttatgc ccctcttaca
    24661 cctgctccgc cagaatccgg tgattgctgc cgttaaagat aatgccagcc tgcaactggc
    24721 catcgattct gaatgccagt ttatttccgt gttgtacggc aatatctgca ccatcagtaa
    24781 tatcgttaaa aagattaaga acgccgggaa atatgctttt attcatgttg atttgctgga
    24841 aggcgcgtca aataaagaag tggtgattca gtttttgaaa ctggtgaccg aggcggatgg
    24901 aattatcagc accaaagcat cgatgctaaa agcggcaaga gcggaagttt ttttctgtat
    24961 tcatcgcctg tttattgttg attcgatttc gtttcacaac attgataagc aagttgcgca
    25021 atcgaatccg gattgtattg agatcctgcc aggctgcatg cccaaagtgc tgggctgggt
    25081 gacggaaaaa atccgccaac cgctgattgc tggcgggctg gtatgcgatg aagaagatgc
    25141 gcgtaatgcg attaacgcgg gtgtcgtggc gctttccacc acgaataccg gagtctggac
    25201 gttagcgaaa aaattgcttt gacgggataa tcgtaaccaa ttgaatttgt tttgatttgt
    25261 tggccgcacg ccacatccga cattcagcgc ctgatgcgac gcttaacgcg tcttatcagg
    25321 cctacaggtc cccagtccag aaccgtaggc aggatcatcc ggcagcacgt tattggtatt
    25381 ctgcgtgaat gtttgtcacc agtgcctcca gcaccacttt ccagtcatca accacgtcaa
    25441 tatctgcctg tgaaaatacc gctgcgctgg cgtcatgatt aatcgccacc ataaacttgc
    25501 tattgcgtac gcccgccatt aacgctgctg cgccggaagc gccaacaaca atgcacacct
    25561 caggtgccag cagatgtccg gaaataccaa tcactttttc agcatcaacg ccaccgttca
    25621 tcactcgcgc ccgactgtag cccacttcgg ttcccagctt ttctgccagc ataacgattt
    25681 cctggttatc tgcctctccg ccctgcccca ccaccagcac ccgtctggcc tcagctaatg
    25741 ggtcgcggct aacatttttc agatcctctg tgctaaccag ccagtcggga agcgcacctg
    25801 ggacaatgtt cagttgctgc attccagagg gcaacgtggc gtttttcgct gctcccgcct
    25861 gacgcgccag ggaaaggcat aacgggcgtt tttctgtttg cagcgtcgcc gttaacgcat
    25921 ttccccagtg ggatttgcgc atgcttacgg ttggaatatc taacgaagtc acctgacaga
    25981 tgcttgcacc atgtaaacgc caggccagcc gggtcgacag ttcatcgcca aacgttcccg
    26041 gcggaaagag aacaacgtcc gcaggcgttc gctgccactg ttccaccatc gcatccagca
    26101 cctgttccgc cactacgggc tgaggttcta tttgccagtg cgccagcgta caaccgctga
    26161 aatcctgcgc cgccagccag ctcgctattg cggcattctc ctgatttatt gtcacaattg
    26221 cgatattcat ggctgcatcc tttggcgtaa atagtcctgc catagctttt gagctttttc
    26281 tgctaccgtt tgcccatcaa tcagcgtcgc tccgcatcgt tgctctgctc ttgccagttg
    26341 cagacactgc atcgctggca tttcggcagc aacagtttta cggataattt ctgctttccc
    26401 cgcagccatt cgctggcgca ttcccggtac tggcaaggcc acttcaccgc actgacgcac
    26461 ggcaatcacc gcaggcaagc gaacccggca acagcgcaac ccatgttcag tacgctgttc
    26521 gagggtgata aacagcgcgt cgagagtgaa acgctccacc agggtgaagc agggccagcc
    26581 cagcatttcc gccagcaaaa atggcgtttg cccattctgc ccttcgctgc tttggcagcc
    26641 ggtaattatc agatccagcg gattctgacg ctgccattcg gcaatatggc gagcgacaaa
    26701 ttccggcgca aagcgcagat ctgccgccgt ctccagcaat acagcttcct caaacccaag
    26761 agccatgaga tagcgcagcc agtgcaacgc ccgttcatcc cccatgctca acgcggttaa
    26821 agacatcggc gtaccgtttt ttctctgcgc taacagcagc gcggcagcgg cctgttcatc
    26881 agcaccgagt aaacttcgca gtagcgaaac atccggtccg ctattaccct gagccgccgc
    26941 ctgccactct ttttccgcca gcattccggc atccggttcg gctttaaacg ctaacagaat
    27001 gttcattcgc acctcctaca tttcccgccg ccaccagcgg gagggcttta gtttctggtg
    27061 cccatagcca ggtcacaaca aaaccaacca acaacactgt cgccaggagc aataaggtga
    27121 cttgcattcc ccactgcgcc agcacccacg gcagcaggcc agtacttacc gccgcgccaa
    27181 gtcgactcat ggcggtggca aaaccgacgc ccagcgagcg aatgtcagta ggaaaacttt
    27241 ccgcaggcaa aatgcccacc agattactga ctgccgaaat ggtggtgctg aagagaacaa
    27301 aaagcagcag cgttaatgaa ctgccggaag gcaaacaggc cattactacc agcgttgccg
    27361 ccagcagcaa aaaacttccc agcaaaaatt tgcgatgtgc cagcaggtgc gtcagaacta
    27421 atcccagcag cgcgcccaca attaacaacg cattaagcat caggctggca gtcagcgcat
    27481 cttccagacc aatagtctgg gcgatagttg gcagccaggt ataaatcaca aaccatggga
    27541 ttacgaggca gacaaagaag atgctgttaa acgccgtgcg ccgccagtaa cgcgaagaga
    27601 acaaggtttt gatgtgttta tgggtcgccg ttaccacttc atcgcccagt aaaacatggg
    27661 gaccaaaata gcgatgcacg atagcgtgag cttctgcaaa acgcccctgg cacagtagcc
    27721 aacgtggcga ttctggcgtt ccccagcgta ataacgtaat caacaacgcg ggcagagctg
    27781 ccgatgccag caaccagcgc caggcctccg ggttttcgga aataaagtga tgtccggcaa
    27841 tacttgccaa cacatagcct acggtccaca ccacgctgaa tgcgcccagc aaaataccgc
    27901 gatggcggcg cggggaaaat tcagccagca aggtgtgacc tactgagtaa tcgcctccca
    27961 gaccaatgcc gatcaaaatg cgcagcccaa taagatgctc tggcgtggtg gcaaaaaatt
    28021 gcaagaacga agcgagcgta atcagcaaaa agctgaaggt gaagattttt tgccgaccaa
    28081 tatggtcgga gatccaccca agaaccaggc tgccaagaaa caaaccaagg agcgccgagc
    28141 cgccgctcat tcccgccata aacggcgtca gttgcatggc gggcgtaagt tgaataatgg
    28201 cgtaaccaat gacgccgaga acataaccgt cggtcaggtg tgcgccgaaa gtgagcgcag
    28261 caattcgaca gtgaaaacgg ttaagcggta aatcatccat tcgcaccggt gaagtgttca
    28321 tctttacccc ttcattgtcc ggtaataaga acggcttctc gcctgagaag ccgaaaaggc
    28381 aacgccccgt catcgcggaa cgctgcctga tacattattt ttcaatcgga tagatagtac
    28441 ccgtattcat aatgccgtta ggatcgaact gctttttcag cccttccagc aacgcccacg
    28501 cgctgccgtg ttccagcttg ctccagtgaa cgcgatgttt accaatgcca tggtggtgca
    28561 ccatcgaacc gccgaggcga atggtttctt cgcagatgat tttgttgagc ggattgtggt
    28621 acttgtcgat ttcctcttcc ggcttacagt caacaacgtt gtagtcatag acgaagtaca
    28681 tgttggtgcc gttctgatag ctatgagagg agtgaccgcc cagcatggtg atgtcgtcgg
    28741 cgtgcgggaa ctcagtacga atacggttaa taacgctttc gtagatttcg tggatgcagc
    28801 tccagcagcc ggacacttcg gtggtaaagc ccatgttgcc ggttttgagg atctgcacac
    28861 gttcggcagc cactttatcc ggtccccagt tcaggttgtt aaaccaggtt tcaatcagtt
    28921 tgctgtccac gcgctggcac tgcgggtagc gggcaacgat ttccgcaatc ccttcgcccg
    28981 tcgccttcgc aatgcgaggg ttaccttcag ccataaagat cagcacccat tttccgtcgg
    29041 caaaatgggt gaagtgttgg gtgccatctt cagcgtcata caaacgagcg atcgacggac
    29101 gatacccttc caccatgatt tcacgcagga tgttgaagcc ggttttcatg tcttccagga
    29161 tatagccgta gaagaggttg ttttccgggg tgtatttaaa gattttcact gttacttcag
    29221 tgatatagca caatgcacct tcgttgccga tgatgatgtg acgaatgtcc gggccagccg
    29281 cgcggcgtgg cacgttttta atgcgtgtga cggtgccatc tgccaacact gcctccagac
    29341 caacgaccat atcttcgatt gcgccgtaga gtgtggagaa ctgcccgata ctgcgggttg
    29401 ctaccaggcc gcccatctgc gccagcggct ttgactgcgg agaatgcccc gtggtgtaac
    29461 ctttttcacg caacgcgttt tccagcactt ccagcggaac accacattgc gccgtcgcct
    29521 gcatattctc aatatcaata ttaatgattt gattcatggc ggagccgtcg agcaccaccg
    29581 agttttctac aacagtttcc agcccacctt cggtggcgga agcaccggta cgcggcacac
    29641 cgttaatttt gtgcgcattc ataaaattca gcacacggga tacttactct gtggaaccga
    29701 gttttacgac cgctgccgga atcggcaaag tataaatgcc atgaatatcc ggaaatttac
    29761 gaaaacggtc aatactgttt ttctttaata ctgtttcatc ggtaattacg cgatctgcac
    29821 caacaatttc ctttagctgg tcgacaatcg ctgcgcgaga taaagacata gtaattcctt
    29881 cctgataata aaaatggaat aatcattaat aagagaaaac agaaaccacc gttatggatt
    29941 agcgcactaa ataaccgcca tcgaccacta ataaatgccc gttgacataa ttcgatgcct
    30001 gacttgcgag aaacacggct gcgcccatta aatcctgagt atcgccccaa cggtttgccg
    30061 gaatatgatc aagaacgcgc tgattggttt ctggattact gcgtgtcgcc agcataatat
    30121 gctgttgcat aatagccagg ggcgatacca tttacctgaa tattatattg acctagttca
    30181 tcacaataag ctttggtgaa cccggcaaga gcatgtttag tggcagaata tgcaggtgac
    30241 cattgtccac ctaagtaaga gaacaatgaa cagatattaa taattttgcc gcttttctgc
    30301 gggatcataa tttttgccgc ttcatagctt aactcgaatg cggcggtcag gttcacatca
    30361 atcatcggat cccagtcggc acgaccgaag tccagcacct tattcagctt acaaataccg
    30421 gcattgttaa ccagaatatc gacagtaccg aagcgctcac agcaggcagc gataatcttc
    30481 tgcggtgcgc cttctgcggt aatatccacc tgcatgaagt ccacttcaac accctgtttt
    30541 tcaatcattt cctttgtttc gccgttatct ttgacgaagc taggaataaa aatatttgcg
    30601 ccagctttgg ccaacgccat tgcaaatgcc tgacctaaac cgctattccc gccggtaaca
    30661 attgctgttt tacctttcag ggagaaaaaa tccattgaga acgcattgag agattcgatt
    30721 gacatagtta gctccaattt ccgtaaggca aaaaaaaaga aaggtaactt cccccgcgag
    30781 agggaagtta cctttctcat tatctctggt aactggagat aatttttagc ataacggcat
    30841 aactgctaat gtgatcgtaa tcacagtgtg atattcgtat tgtcaaatag ttttaaaaag
    30901 cagaagcgtg attactctca cacaacacct ctgctttcca tgttatttct tattgcaatt
    30961 actagagacc tgagaaaagt aatccacctg aaagggcggc ttacttttct cttttttttg
    31021 tctattacaa atgggaatac gatatgcaac acaactcata tcgccgttgg ataaccctcg
    31081 cgataattag ttttagcggc ggcgttagtt ttgacctggc ttatttacgt tatatttatc
    31141 aaattcccat ggcgaaattt atgggattca gcaataccga gataggttta ataatgagta
    31201 cctttggtat tgcggctatt attctttatg cccccagcgg cgttattgcc gataaatttt
    31261 cacaccgcaa aatgattact tccgcgatga tcattaccgg attactgggt ctgttaatgg
    31321 caacgtatcc accgctgtgg gtaatgctct gtattcaggt cgcctttgcg ataacgacga
    31381 ttttaatgct gtggtcggtg tcgattaaag ccgcctcgtt gcttggcgat catagcgagc
    31441 aagggaaaat tatgggctgg atggaagggc tgcgcggcgt cggtgtaatg tcgctggcgg
    31501 tgtttaccat gtgggtcttt tctcgctttg caccggatga cagcgccagc ctgaaaacag
    31561 tcattatcat ctacagtgtg gtttacatct tgttggggat tctgtgctgg ttttttgtta
    31621 gcgataacaa caacctgcgc agtgccaata acgaagaaaa acagtcattc cagcttagcg
    31681 acatccttgc cgttttgcgt atcagcacca cctggtattg cagcatggtg atttttggtg
    31741 tcttcacaat ctacgccatt ctgagttact ccaccaacta tctgaccgaa atgtacggca
    31801 tgtcgctggt ggcagcgagc tacatgggga ttgtgatcaa caaaatcttc cgcgcgctgt
    31861 gcggcccact tggaggcatt atcaccacct acagtaaagt gaaatcccct acccgcgtga
    31921 ttcaaatcct ttccatcatc ggtttgctgg cgttgacagc cctgctcgtc acgaactcta
    31981 acccgcaatc ggtcgcgatg gggattggcc tgattttact gctgggattc acctgttacg
    32041 cctcacgcgg gctgtactgg gcctgccccg gcgaagcgag aacaccgtct tacattatgg
    32101 gcaccacggt aggtatttgt tcggtgattg gattcctgcc ggatgtcttc gtttacccga
    32161 ttatcggcca ctggcaagac accctgcccg ctgcagaagc ctaccgcaat atgtggctga
    32221 tgggcatggc ggcgcttgcc atggtgatag tctttacctt tttgctgttc caaaaaattc
    32281 gtactgctga tagcgccccc gcaatggcta gcagcaagta agccaggaaa attacaggag
    32341 aaaaatcatg tcgaaaaaat acatcatagg gattgatggc ggaagtcaga gcacaaaagt
    32401 ggtgatatac gatctggaag gtaatgtggt ttgcgaaggg aaaggcttat tgcagccgat
    32461 gcacacgcca gatgccgata ctgcagaaca tcctgacgac gatttatggg catcattatg
    32521 ttttgccggt cacgatttga tgagtcagtt tgccgggaat aaagaagata ttgtcggtat
    32581 tggtctggga tccatccgtt gctgccgtgc gttattgaaa gccgatggca cggccgttga
    32641 ttagctggca ggatgcacgc gtaacgcgtc cctacgaaca cactaatcct gacgtggcat
    32701 atgtcacctc tttttcgggt tatctgacgc atcgcttaac cggcgagttt aaagacaata
    32761 tcgccaacta ttttggtcag tggccggtgg attataagag ctgggcatgg agcgaagatg
    32821 ctgcggtaat ggagaagttt aatatccccc gtcatatact gtttgatgtg caaatgccag
    32881 gcactgttct tgggcatatc acaccacaag ccgcgctggc gacacatttc ccagctgggc
    32941 tgccggttgt ttgtaccacc agtgataaac cggtagaagc tctgggggcc ggattactgg
    33001 atgatgaaac ggcggcgatt tcactcggta cttatatcgc gctgatgatg aacggcaaag
    33061 ccttgccgaa agatccggtg gcgtactggc cgattatgtc ttctattccg caaacattgc
    33121 tgtatgaagg ttacggtatt cgcaaaggta tgtggacggt gagctggctg cgcgacatgt
    33181 taggcgagtc gttaattcag gatgccaggg cgcaggatct ttcaccggaa gatttactca
    33241 acaagaaagc ctctagtgtg ccacctggct gtaatgggct gatgacggtg ctggattggc
    33301 tgaccaatcc gtgggaaccg tacaaacgcg ggattatgat cggctttgat tccagcatgg
    33361 attacgcatg gatatatcgt tcgatactgg aaagcgtggc gctgacgctg aagaacaatt
    33421 acgacaatat gtgtaatgaa atgaatcact ttgcgaagca tgtgatcatt actggcggcg
    33481 gttcgaacag cgatctgttt atgcagattt ttgccgacgt gttcaacctt ccggcacgac
    33541 gtaacgccat taacggttgt gcaagcctgg gggcagcgat taatacagcg gtaggtctgg
    33601 ggctataccc ggattacgca acggctgtcg acaaaatggt tcgcgtgaaa gatatcttta
    33661 tgcctgttga gagcaatgcc aaacgctacg acgcgatgaa taaaggtatt ttcaaagacc
    33721 taaccaaaca tactgatgtg atcctgaaaa aatcgtatga agtgatgcat ggggaattgg
    33781 ggaatgcgga ttcgatccag agctggtcga atgcgtaagt gaggaagacc gggcgggaaa
    33841 ctgcccggcc tgaatatacc tgaatggtta tccccgctga cgcggggaac tcgaataact
    33901 gaccaacgtc tggctctcta aatccggttt atccccgctg gcgcggggaa cactctaaac
    33961 ataacctatt attaattaat gattttttaa gccagtcaca atctaccaac tttacaggat
    34021 cacacaaaca acacatccat tatgttaaag agcacttaat ccattgatta aaaaggtaaa
    34081 tatttaaaat aactctatac aaactaaaat ctaccaaact tcgccgcaat aattttcact
    34141 ccagcgaaaa attaacgccg cagaatttgt agagaatgat aatgattagt ccgggatggg
    34201 caagatggat gagttttcag gggcgacaaa taatacaaat gagtggaatt ttttacaagt
    34261 tccacccatt tataa
//
LOCUS       DADMGC010000028        33271 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.028, whole genome shotgun sequence.
ACCESSION   DADMGC010000028 DADMGC010000000
VERSION     DADMGC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33271
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.028"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            70..1347
                     /gene="waaA"
                     /locus_tag="JNZ73_001341"
     CDS             70..1347
                     /gene="waaA"
                     /locus_tag="JNZ73_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HAY8731674.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1355..1834
                     /gene="coaD"
                     /locus_tag="JNZ73_001342"
     CDS             1355..1834
                     /gene="coaD"
                     /locus_tag="JNZ73_001342"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAY8731675.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1873..2682)
                     /gene="mutM"
                     /locus_tag="JNZ73_001343"
     CDS             complement(1873..2682)
                     /gene="mutM"
                     /locus_tag="JNZ73_001343"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HAY8731676.1"
                     /translation="MPELPEVETSRRGIEPNLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2780..2947)
                     /gene="rpmG"
                     /locus_tag="JNZ73_001344"
     CDS             complement(2780..2947)
                     /gene="rpmG"
                     /locus_tag="JNZ73_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAY8731677.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2968..3204)
                     /gene="rpmB"
                     /locus_tag="JNZ73_001345"
     CDS             complement(2968..3204)
                     /gene="rpmB"
                     /locus_tag="JNZ73_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAY8731678.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3421..4089)
                     /locus_tag="JNZ73_001346"
                     /pseudo
     CDS             complement(3421..4089)
                     /locus_tag="JNZ73_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            4261..5481
                     /gene="coaBC"
                     /locus_tag="JNZ73_001347"
     CDS             4261..5481
                     /gene="coaBC"
                     /locus_tag="JNZ73_001347"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAY8731679.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5459..5917
                     /gene="dut"
                     /locus_tag="JNZ73_001348"
     CDS             5459..5917
                     /gene="dut"
                     /locus_tag="JNZ73_001348"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAY8731680.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6024..6620
                     /gene="slmA"
                     /locus_tag="JNZ73_001349"
     CDS             6024..6620
                     /gene="slmA"
                     /locus_tag="JNZ73_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HAY8731681.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6657..7298)
                     /gene="pyrE"
                     /locus_tag="JNZ73_001350"
     CDS             complement(6657..7298)
                     /gene="pyrE"
                     /locus_tag="JNZ73_001350"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAY8731682.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7364..8080)
                     /gene="rph"
                     /locus_tag="JNZ73_001351"
     CDS             complement(7364..8080)
                     /gene="rph"
                     /locus_tag="JNZ73_001351"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAY8731683.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8207..9070
                     /locus_tag="JNZ73_001352"
     CDS             8207..9070
                     /locus_tag="JNZ73_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAY8731684.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9291..10115
                     /gene="dinD"
                     /locus_tag="JNZ73_001353"
     CDS             9291..10115
                     /gene="dinD"
                     /locus_tag="JNZ73_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HAY8731685.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            10406..11023
                     /locus_tag="JNZ73_001354"
     CDS             10406..11023
                     /locus_tag="JNZ73_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HAY8731686.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11020..12537)
                     /gene="ligB"
                     /locus_tag="JNZ73_001355"
     CDS             complement(11020..12537)
                     /gene="ligB"
                     /locus_tag="JNZ73_001355"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HAY8731687.1"
                     /translation="MEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTG
                     VRKMADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQK
                     VSLISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTL
                     NSLAVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLP
                     FVTDGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVA
                     SLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLGGQGIPRIDDVVWRGAERTK
                     PTPPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEH
                     IFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASD
                     ERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(12559..12816)
                     /locus_tag="JNZ73_001356"
     CDS             complement(12559..12816)
                     /locus_tag="JNZ73_001356"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731688.1"
                     /translation="MIYCNKDDSQEASSQTPFLPLGFAFTTLYICQPDYSGGEDESMD
                     GDINKYLVLAIICVGGLSGLVASQSTGRNFPPATANKTVGR"
     gene            12956..13579
                     /gene="gmk"
                     /locus_tag="JNZ73_001357"
     CDS             12956..13579
                     /gene="gmk"
                     /locus_tag="JNZ73_001357"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAY8731689.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13634..13909
                     /gene="rpoZ"
                     /locus_tag="JNZ73_001358"
     CDS             13634..13909
                     /gene="rpoZ"
                     /locus_tag="JNZ73_001358"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAY8731690.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            13928..16036
                     /gene="spoT"
                     /locus_tag="JNZ73_001359"
     CDS             13928..16036
                     /gene="spoT"
                     /locus_tag="JNZ73_001359"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HAY8731691.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16043..16732
                     /gene="trmH"
                     /locus_tag="JNZ73_001360"
     CDS             16043..16732
                     /gene="trmH"
                     /locus_tag="JNZ73_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HAY8731692.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16738..18819
                     /gene="recG"
                     /locus_tag="JNZ73_001361"
     CDS             16738..18819
                     /gene="recG"
                     /locus_tag="JNZ73_001361"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAY8731693.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18853..20058)
                     /gene="gltS"
                     /locus_tag="JNZ73_001362"
     CDS             complement(18853..20058)
                     /gene="gltS"
                     /locus_tag="JNZ73_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HAY8731694.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20338..21729
                     /gene="xanP"
                     /locus_tag="JNZ73_001363"
     CDS             20338..21729
                     /gene="xanP"
                     /locus_tag="JNZ73_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HAY8731695.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSDFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21850..23559
                     /locus_tag="JNZ73_001364"
     CDS             21850..23559
                     /locus_tag="JNZ73_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY8731696.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     QLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23612..25930)
                     /gene="yicI"
                     /locus_tag="JNZ73_001365"
     CDS             complement(23612..25930)
                     /gene="yicI"
                     /locus_tag="JNZ73_001365"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HAY8731697.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEYFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25940..27323)
                     /locus_tag="JNZ73_001366"
                     /pseudo
     CDS             complement(25940..27323)
                     /locus_tag="JNZ73_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27616..27710
                     /locus_tag="JNZ73_001367"
     tRNA            27616..27710
                     /locus_tag="JNZ73_001367"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27650..27652,aa:Sec,seq:tca)
     gene            27982..29194
                     /locus_tag="JNZ73_001368"
                     /pseudo
     CDS             27982..29194
                     /locus_tag="JNZ73_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            29606..30649
                     /locus_tag="JNZ73_001369"
     CDS             29606..30649
                     /locus_tag="JNZ73_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAY8731698.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSRIQYQ"
     gene            31164..31625
                     /locus_tag="JNZ73_001370"
     CDS             31164..31625
                     /locus_tag="JNZ73_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731699.1"
                     /translation="MDENALRFASYWRNSLADAESGKGCFERKDAQNFTHWHGIAAGR
                     LDEAIVNKFFKGEKDDVETVNVILRPKVYFRLLQHGKDRSAGTPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTDPTHVMWTRP"
     gene            complement(31622..32477)
                     /locus_tag="JNZ73_001371"
                     /pseudo
     CDS             complement(31622..32477)
                     /locus_tag="JNZ73_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(32474..32773)
                     /locus_tag="JNZ73_001372"
     CDS             complement(32474..32773)
                     /locus_tag="JNZ73_001372"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8731700.1"
                     /translation="MTKPVSISKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYES
                     QLYAWRSKQQQQMSSSERESELAAENVRLKRQLAEQAEELSILQKAATYFAKRLK"
BASE COUNT         8326 a         8751 c         8212 g         7982 t
ORIGIN      
        1 gaaaagtaat ggtaaagcca cagctaaata catagaatcc ccagcacatc cataagtcag
       61 ctatttacta tgctcgaatt gctttacacc gcccttctct accttattca gccgctgatc
      121 tggatacggc tctgggtgcg cggacgtaag gctccggcct atcgaaaacg ctggggtgaa
      181 cgttacggtt tttaccgcca tccgctaaaa ccaggcggca ttatgctgca ctccgtctcc
      241 gtcggtgaaa ctctggcggc gatcccgttg gtgcgcgcgc tgcgtcatcg ttatcctgat
      301 ttaccgatta ccgttacaac catgacgcca accggttcgg agcgcgtaca atcggctttc
      361 gggaaggatg ttcagcacgt ttatctgccg tatgacctgc ccgatgcgct caaccgtttc
      421 ctgaataaag tcgaccccaa actggtgttg attatggaaa ccgaactatg gcctaacctg
      481 attgcggcgc tacataaacg taaaattccg ctggtgatcg ctaacgcgcg actctctgcc
      541 cgctcggccg caggttatgc caaactgggt aaattcgtcc gccgcttgct gcgtcgtatt
      601 acgctgattg ctgcgcaaaa tgaagaagat ggtgcacgtt ttgtggcgct gggcgcaaaa
      661 aataaccagg taaccgttac cggtagcctg aaattcgata tttctgtaac tccgcagttg
      721 gctgctaaag ccgtgacgct acgccgccaa tgggcaccac accgcccggt atggattgcc
      781 accagcactc acgaaggtga agagagcgtg gtcattgccg cacatcaggc attgttacag
      841 caattcccga atttattgct catcctggta ccccgtcatc cagaacgctt cccggatgcg
      901 attaaccttg tccgccaggc tggactaagc tatatcacac gctcttcagg ggaagtcccc
      961 tccaccagca cgcaggttgt ggttggcgat acgatgggcg agttgatgtt actgtacggc
     1021 attgccgatc tcgcctttgt tggcggttca ctggttgaac gtggtgggca taatccgctg
     1081 gaagctgccg cacacgctat tccggtattg atggggccgc atacttttaa ctttaaagac
     1141 atttgcgcgc ggctggagca ggcaagcggg cttattaccg ttaccgatgc cactacgctt
     1201 gcaaaagagg tttcctcttt actcaccgac gccgtttacc gtagtttcta tggccgtcat
     1261 gccgttgaag tactgtatca aaaccagggc gcgctacagc gtctgcttca actgctggaa
     1321 ccttacctgc caccgaaaac gcattgaggt tgttatgcaa aaacgggcga tttatccggg
     1381 tactttcgat cccattacca atggtcatat cgatatcgtg acgcgcgcca cgcagatgtt
     1441 cgatcacgtt attctggcga ttgccgccag ccccagtaaa aaaccgatgt ttaccctgga
     1501 agagcgtgtg gcactggcac agcaggcaac cgcgcatctg gggaacgtgg aagtggtcgg
     1561 gtttagtgat ttgatggcga acttcgcccg taatcaacac gctacggtgc tgattcgtgg
     1621 cctgcgtgcg gtggcggatt ttgaatatga aatgcagctg gcgcatatga atcgccactt
     1681 aatgccagaa ctggaaagtg tgtttctgat gccgtcgaaa gagtggtcgt ttatctcttc
     1741 atcgttggtg aaagaggtgg cgcgccatca gggcgatgtc acccatttcc tgccggagaa
     1801 tgtccatcag gcgctgatgg caaaattagc gtagcgttta tgccggatgg tatgccatcc
     1861 ggctcgcatg aattacttct ggcactgccg acaataaaac gttgcccgct gcgcatgttt
     1921 agtcgccaca atcggcgtac cgcacacccg acacggctca ccttttcgcc cgtaaacctg
     1981 caattcctgc gcgaaatagc ccggtttacc atcactttgc agaaaatctt tcagcgttgt
     2041 accaccctgc tcaatcgaac gcagcaacac cgctttaatc acccgagcta acaattcaca
     2101 ctctgccagc gacagtgatg acgccagccg atccggatgg atccccgccg caaacagtga
     2161 ttcgctggca tagatattcc ctacccctac caccagcttg ttatccatca gccacggttt
     2221 aatcgccgtt tttttcttcg cgcacttctg atgcagatac tcaccattga aatcgtcgct
     2281 aagcggctcc ggtccaagat gggccagcac attatgccct tccagctctt tggtccacag
     2341 ccaggcacca aagcggcgcg gatcggtgta gcgcagcact ttaccgttgc tcatcaccaa
     2401 atccacatgg tcatgctttt cagggggaag ttcttctgga aggatgcgca ggctgccaga
     2461 catccccaaa tgaatgataa tccagccctc aggcagctcc agcagcagat atttagcccg
     2521 ccgctgcacg ctaagcactg gttgatcgct taatcggtag atctcttctg aaaccggcca
     2581 gcgcaagcgt ccgttgcgca ccaccgcatg aagaatggtt gcaccaacga gattcggttc
     2641 tatgccgcga cggctggttt caacttcggg taattcaggc atagcatctc caggaatgaa
     2701 cagatgcaat caatatgggg gcaagcagat aacaaaaaac cccgccggag cgaggttttt
     2761 cgttacatca aagcgagaat tatttgattt tcgcttcttt gtagatcacg tgctgacgaa
     2821 caactggatc gaattttttc agttccagtt tttccggctt agtacgtttg ttcttcgtag
     2881 tggtatagaa gtgaccagta ccagcagaag aaaccagctt gattttctca cgaatacctt
     2941 tagccatgat ttatttcctc taagtactta gtacttttcg ccacgggcac gcagttcagc
     3001 cagaactgta tcgatgcctt ttttatcgat tacacgcata cctttagcag atacacgcag
     3061 ggtgacaaaa cgcttctcgc tctcaaccca gaaacggtga gagtgcaggt tcggcaggaa
     3121 acggcgttta gtcgcgttca gtgcgtggga acggttgtta ccggtcaccg gacgcttgcc
     3181 agtaacttgg cagactcggg acatgtctat tctccaaaaa tcaaattagc tcgagcttcg
     3241 tatggggtat tggcgcctcg tcaggcttta cagcccggtc atcgcagttc tatgtgaact
     3301 ctcgattgcc aggcccaaat gccaaacccg agattctcaa aggtggcgta gtatacgctg
     3361 actcagcgat gtgctcaagt cccgaacaga caaagatccc gaaggatcgc gcatagcggg
     3421 ttaaatccag ccgcgttcgg caaaagaaac atactctcca cgcccaatca cgatatggtc
     3481 gagcacgcgt aaatccatga actgacaact ctttattatc cgttcagtaa tgagtttatc
     3541 cgctttactg ggttcagcac aacccgaagg gtgattatgt gcaaggatca gcgccgaggc
     3601 gtttattttt atcgcttagc ggataatttc ccgaggatgg acttcaacat ggtttagcgt
     3661 gccggaaaaa agacggctat gcgttataac ccggtgttgt gagtcgagaa agatcaccat
     3721 aaagatctcc cgctcctcac ccgtgagctg gctttgtaaa aattcccgcg tcatctccgg
     3781 gctgagtaaa gggctttctt cacgcatccg cacgttgtag taacgtcgcg ccagttcagc
     3841 aatccccttt aactgggcaa atttcgctac gccaattcca tgaacaccac taaattgttc
     3901 atattcagag gttaacaagc cataaagaga gccgaaattc tccagcatct cttttgccag
     3961 ggttaataca tctttaccgc gcgttccggt acgcagaaat agcgccagca gctcgacatc
     4021 cgttaaggcg ctaataccaa acttcagcat tttttcgcgc ggcatcaaca gctgtgcatt
     4081 gtttttcacc ttcacctcct ttgtggtgct cgcatcctgt catagccgtt ccgttaaatc
     4141 gacggccagt tttcaatcct ggaaagagcc tcgcaaagtg aatctcaggt gaaggcacga
     4201 caacaaaagt attgtgataa aatcgccaac ttctggtgtc acccaacagg aaaaatcatc
     4261 atgagcctgg ccggtaaaaa aatcgttctc ggcgttagcg gcggtattgc tgcctataaa
     4321 acccctgaac tggtgcgtcg tttgcgcgat cgcggggccg acgtccgcgt agccatgacc
     4381 gaagcggcaa aagcctttat caccccactt agcttgcagg cggtttctgg ttatcccgtt
     4441 tccgacagtc tgcttgaccc ggcagccgaa gcagctatgg gccatattga gctgggtaaa
     4501 tgggctgatt tagtgattct cgcccctgcc acggcagatt tgattgcccg tgttgctgcc
     4561 ggaatggcga atgacctggt atcgacgatt tgtctggcta cacctgcgcc tgtagccgtg
     4621 ctccccgcca tgaaccagca gatgtaccgt gccgctgcca cgcagcataa tttagaggtg
     4681 cttgcttccc gtggtttgct catctggggg ccagacagtg gcagtcaggc ttgtggtgat
     4741 atcggtcctg gacgaatgct cgatccgtta accattgtgg atatggcggt ggcgcatttt
     4801 tcgcccgtca acgacctgaa acatctgaac attatgatta ccgccggtcc gacgcgtgaa
     4861 ccgctcgatc cggtgcgtta tatctctaat cacagctccg gcaagatggg ttttgctatc
     4921 gccgccgctg ctgcccgtcg tggcgcgaac gtcacgctgg tatcaggtcc agtttcacta
     4981 ccgacgccac cgtttgttaa acgtgttgat gtgatgaccg cgctggaaat ggaagccgcc
     5041 gtgaatgctt ctgtacagca gcaaaatatt tttatcggtt gcgccgccgt ggcggattat
     5101 cgcgcagcta ccgtggcccc agaaaaaatc aaaaagcagg ccacgcaggg tgatgaatta
     5161 acaataaaaa tggttaaaaa ccccgatatc gtcgcaggcg ttgccgcact aaaagaccat
     5221 cgaccctacg tcgttggatt tgccgccgaa acaaataatg tggaagaata cgcccggcaa
     5281 aaacgtatcc gtaaaaacct tgatctgatc tgcgcgaacg atgtttccca gccaactcaa
     5341 ggatttaaca gcgacaacaa cgcattacac cttttctggc aggacggaga taaagtctta
     5401 ccgcttgagc gcaaagagct ccttggccaa ttattactcg acgagatcgt gacccgttat
     5461 gatgaaaaaa atcgacgtta agattctgga cccgcgcgtt gggaaggaat ttccgctccc
     5521 gacttatgcc acctctggct ctgccggact tgacctgcgt gcctgtctcg acgacgccgt
     5581 agaactggct ccgggtgaca ctacgctggt tccgaccggg ctggcgattc atattgccga
     5641 tccttcactg gcggcaatga tgctgccgcg ctccggattg ggacataagc acggtatcgt
     5701 gcttggtaac ctggtaggat tgatcgattc cgactatcag ggccagttga tgatttccgt
     5761 gtggaaccgt ggtcaggaca gcttcaccat tcaacctggc gaacgcatcg cccagatgat
     5821 ttttgttccg gtagtacagg ctgaatttaa tctggtggaa gatttcgacg ccaccgaccg
     5881 cggtgaaggc ggctttggtc actctggtcg tcagtaacac atacgcatcc gaataacgtc
     5941 ataacatagc cgcaaacatt tcgtttgcgg tcatagcgtg ggtgccgcct ggcaagtgct
     6001 tattttcagg ggtattttgt aacatggcag aaaaacaaac tgcgaaaagg aaccgtcgcg
     6061 aggaaatact tcagtctctg gcgctgatgc tggaatccag cgatggaagc caacgtatca
     6121 cgacggcaaa actggccgcc tctgtcggcg tttccgaagc ggcactgtat cgccacttcc
     6181 ccagtaagac ccgcatgttc gatagcctga ttgagtttat cgaagatagc ctgattactc
     6241 gcatcaacct gattctgaaa gatgagaaag acaccacagc gcgcctgcgt ctgattgtgt
     6301 tgctgcttct cggttttggt gagcgtaatc ctggcctgac ccgcatcctc actggtcatg
     6361 cgctaatgtt tgaacaggat cgcctgcaag ggcgcatcaa ccagctgttc gagcgtattg
     6421 aagcgcagct gcgccaggta ttgcgtgaaa agagaatgcg tgagggtgaa ggttacacca
     6481 ccgatgaaac cctgctggca agccagatcc tggccttctg tgaaggtatg ctgtcacgtt
     6541 ttgtccgcag cgaatttaaa taccgcccga cggatgattt tgacgcccgc tggccgctaa
     6601 ttgccgcgca gttgcagtaa tatgacgccg gatgactttt catccggcga gtttctttaa
     6661 acgccaaact cttcgcgata ggccttaacc gccgccagat gttccgccat ttccggcttc
     6721 tcttccagat aggcaatcag atctttcagg gtgatgatag agatcacttt gcagttgtaa
     6781 tcacgctcaa cttcctgaat cgccgaaatc tcgccgcgcc cgcgttcctg acgatcgagc
     6841 gaaatcaaca cgccagcaag cgtcgcgcca ttggcctgaa taatctccat cgactcgcga
     6901 atcgccgttc cggcggtgat cacatcatct accagcatta cgcgtccttg taacgcgcta
     6961 ccaaccagat tgccgccttc accgtggtct tttgcttctt tgcggttaaa gcagtacggc
     7021 aggtccaggt cgtgatgctc cgccagtgcc acagcggttg tggtggcaat cgggatccct
     7081 ttgtaagcag ggccaaacag cagatcgaac tcaatgccgg aatccaccaa cgcttcagcg
     7141 taaaaacggc ctaacagtgc cagatcgcgc ccggtattaa acagcccggc gttgaagaaa
     7201 taggggcttt tgcgcccgga tttcagcgta aactcgccaa actttaacac ctgcttgcta
     7261 agcgcaaatt caataaactg gcgctgatat ggtttcatgc cttcgctcct catcttactt
     7321 ttctacagac aaaaaaaagg cgactcatca gtcgccttaa aaatcagttt gccagcgccg
     7381 ccttctgcgt cgctacaatg gattcgattc cccctcgggc cagcgccaac aaggtgagta
     7441 gctcttcatg ggtgaacggc tcgccttctg ccgtcccctg cacttcaatg atgcgcccgt
     7501 cttcggtcat cactacgttc atgtcggtct ctgcggctga gtcttcaacg tattccagat
     7561 cgcaaattgc ttcaccgttt acaataccga cagaaactgc ggctaccatc cctttcatcg
     7621 ggttggtttt cagcttgccg ttttccacca gcttctgtag cgcatctgcc agcgccacgc
     7681 aggcacccgt aatcgacgcg gtacgagtgc caccatcagc ctgaagcacg tcgcagtcca
     7741 gcgtaatggt gaactcaccc agcgctttca aatctactgc cgcgcgaaga gcacgggcga
     7801 tcagacgctg gatttccatt gtgcgtccac cctgcttacc tttcgccgct tcacgagcgt
     7861 tacgggtgtg ggtagaacgt ggcagcatgc cgtactctgc ggtgatccag ccctggccct
     7921 gacctttcag gaagcgcggc acgccttctt caatagaggc ggtacacaat actttggtat
     7981 cgccaaattc gaccagcacc gagccttctg catgttttgt atagttacga gtcagggtaa
     8041 cgggacgcac ctgattattg ctacggcctg ctggacgcat attgaaatct ccggcttgaa
     8101 acaaatgtgg ctgcgcatta tacggacttc cggcggttat tcctatcctg acaaggcatc
     8161 gatggctata atccttccac ctctcctttt ataaacagga acgtctatga tccgcagtat
     8221 gaccgcctac gcccggcgtg aaatcaaggg tgaatggggg agcgcaacct gggaaatgcg
     8281 ctcggtaaac cagcgttatc tggaaactta ctttcgtctg ccggagcagt tccgtagcct
     8341 tgaacctgtc gttcgcgagc gtattcgttc tcgcctgacg cgcggtaaag tggaatgtac
     8401 cctgcgctat gagccagatg ttagcgcaca aggtgagctg atcctcaatg aaaaactggc
     8461 taaacagctg gtaactgccg cgaactgggt aaaaatgcag agtgacgaag gggaaatcaa
     8521 cccggttgat attctacgct ggccgggcgt gatggcagcc caggagcagg atctcgacgc
     8581 gattgccgct gaaattctcg cggcgttgga tggtacgctg gacgacttta tcgtcgcgcg
     8641 cgaaaccgaa ggtcaggcac tgaaagcatt gatcgagcag cgtctggaag gcgtcaccgc
     8701 cgaagtggtc aaagtccgcg cccatatgcc ggaaattctg caatggcagc gtgagcgtct
     8761 ggtcgcgaag ctggaagatg ctcaggttca gttggaaaac aaccgtctgg agcaggagtt
     8821 ggttctgctg gcacaacgaa ttgacgttgc cgaagagctg gaccgcctcg aagcgcatgt
     8881 taaagagacg tacaacattc tgaagaaaaa agaagcggtt ggtcgtcgtc tggactttat
     8941 gatgcaggag ttcaaccgtg agtcgaacac tcttgcgtcg aaatctatca atgccgaagt
     9001 gacaaactcc gccatcgagc tgaaagtgtt gattgagcag atgcgcgagc agattcagaa
     9061 tattgagtaa ctcgtaacac caattctgac ttttcagcct tcacaaatgc cgcccactca
     9121 aacagagcgg catttttctt ccccgcaact tcaattctgt tttgcgtgcc tgctccagat
     9181 tttgcgatgt ttttttgccc agcacactga gaacgtgaga tactcacaac tgtatataaa
     9241 tacagttaca gatttacttt ctttgcaatc gatatcacat ggagtgggca atgaacgaac
     9301 atcatcaacc ttttgaagag ataaaactga ttaatgcaaa cggagcagaa caatggtcag
     9361 caagacaact tgggaaacta ctgggttatt cagagtatcg tcactttata cctgtattaa
     9421 cgcgcgccaa agaggcctgt gagaacagtg gtcacacaat tgatgaccat ttcgaggaga
     9481 tcctcgatat ggtcaaaatt ggctcaaatg ccaaaagagc attaaaagac atcgtactct
     9541 cccgctatgc ctgttacctg gtagtacaaa acggcgaccc cgcgaaaccg gtcattgcgg
     9601 cagggcagac ttattttact atccagaccc gacggcagga gcttgctgac gatgaagcat
     9661 ttaagcaact tcgtgaagac gaaaagcgtc tgtttctgag aaatgagttg aaagaacata
     9721 acaaacaatt ggttgaggca gcacagcaag ctggggtagc gacagctact gatttcgcca
     9781 tatttcagaa tcatggttac caggggctgt atggtggatt agatcagaaa gccatccacc
     9841 aactgaaggg gctgaaaaag agtcagaaga tcctggatca tatgggttca acagaactgg
     9901 cggctaatct ctttcgagct acccaaacag aagaaaaact caagcgggat agcgtgaatt
     9961 caaaacagca agctaatacc acacactttg acgtgggtag caaggtgagg caaaccattc
    10021 aggaacttgg cggaaccatg cctgaggagt tgccgacccc gcaggtcagc atcaagcagt
    10081 tggaaaacag tgtaaaaatt acagagaaga aatagcagaa acgaaaaact ctttttgttg
    10141 attgagacac ccgatgcgta agccaaggtc caggtgcagt gaacacaatg gctaaacatt
    10201 gcacctttct tttcccccct cagttttaac ctattttttc ttatgcattt tctcagacaa
    10261 gaagtcagaa gaatgcatct acgctacaga aaatagcgat ttcacataac taaaagttat
    10321 caatttcctc tcaccttaaa aaatatcaat cgtgacaatg cgcacaaatc gctaccctgc
    10381 cagacagatt tttagggaga gaaccatgct gttacacatt ttgtatttgg ttggcattac
    10441 tgccgaagcc atgacggggg cgctggcggc cgggcgacgg cgcatggata catttggcgt
    10501 aattattatt gcaaccgcca ccgcaattgg cggagggtca gtgcgcgata ttctgctggg
    10561 ccactatccg ctcggctggg tcaaacaccc ggaatatgtg attatcgtcg ctaccgccgc
    10621 agtactcacc actatcgttg ctcctgtaat gccttacctg cgcaaagtgt ttctggtgct
    10681 cgatgcgctc ggactggtgg tcttttctat catcggcgca caggttgcac tggatatggg
    10741 gcacggccca attattgccg ttgtcgcggc ggtaaccacc ggtgtatttg gcggcgtttt
    10801 gcgcgatatg ttctgtaaac gcattccgct ggtctttcaa aaagagttgt atgccggtgt
    10861 ctcatttgcc tccgccgtgc tgtacatcgc gctgcaacac tatgtttcta accatgatgt
    10921 ggtgattatc tccaccctgg tattcggctt ttttgctcgt ttactggcgc tacgtcttaa
    10981 gctgggatta ccggtctttt actacagcca cgaaggtcac taaggttcaa aacctgtgat
    11041 ctgctgggca gccagccaac tgcccagctt cttgatttgc gcattttcct tccattcaat
    11101 aacctgtctg gcgcgcccag atccagtccc cggcagctgc tgccagaact gctccgtgct
    11161 aaataaaagt tgcgaccagg atcgttcatc actggcatta agcgccgccc gggttagcgg
    11221 tattcccatt gccatcaccc agcgagtaaa aggctggtta cgagccagat taaactgatg
    11281 ccataactgc gcacttttac ttttcgcgat ccccggcgtg ttctgtaatt gctctggcgt
    11341 taataaaagc caggaaaaga tatgctcaaa gcgatgagtc tgatgcagcg cgcgccaacc
    11401 ggcctcacca atgccatcca gcccgagaac ctgttttgac cccagccaga ctaagcgtga
    11461 aatgaactgt tcctgacaaa catcagaagc aaagtagcag gtcaacgagt taaagcggtt
    11521 ttctggtggt gtcggttttg tacgttctgc accgcgccag accacatcat caatgcgagg
    11581 aataccctga ccgccaaggc tgacgaggat ctgatcacca ggcgcaatat cccactcctg
    11641 ccagcgcctg acggaaccaa tattcacccg ctggactttt ttatcatcca gcatgacagg
    11701 tgcgagtgac gcaaccaccg atattttacc gctcttaccc accgcaaact gaatcgcctt
    11761 cacttcggca acctgagcta caggttgata tttccaggcc accagccact ccgcctggcc
    11821 cggtagccaa tgacgggatt cgggttcctt agcccctcgc acaaccacgc catcggtgac
    11881 gaagggtaat ttcgctttcc accactcatt gcgtacgcgc gcaacctcat cagcattttt
    11941 aaccgcacgg gtatacctct gcgttagagt aaaacctgcg gtagccagct cctttaaacg
    12001 atcggtcatt aactgcggtc catccggcca tgcccagaca aaaacagcca gtgagtttag
    12061 cgtgtcgcta ttcccctggc gcatcatcag gccagcaact tttgcgcggg catttattcc
    12121 ccccatttgt tgttggatat gcccctcgcg ctggagaaat atttccccct gaagcgtact
    12181 attggctaaa ggcccgctaa cggtttgcgg cacagcggaa attaagctaa ctttctgcgt
    12241 ccagtcctcg cctttcaggc cattaccgcg actgattgct ttgttcagtt tcccgtcccg
    12301 ataaaccagg gttaccgcaa cgccatcaac ttttggctgc acccaaagat cactacgttc
    12361 tcgcatccac agacttaatg cgtttttatc cgccattttg cgtacacccg tatgggcaac
    12421 cggatgcata accgcaccat ttaaaggcgg catcatcacg tcgcggggct cgctcacaaa
    12481 gcagcgttgc cactgcgtaa gacgggcgct taactgatcg taaacaccgt cttccacctc
    12541 actttttcct tccagtagtc atcgtcccac tgttttattt gctgttgcag gcgggaaatt
    12601 tcttcctgtg ctctggctgg cgaccaggcc ggacagaccg cccacacaga tgattgccag
    12661 cacaagatac ttattaatat cgccatccat actttcatct tcacctccgc tgtagtcagg
    12721 ctggcagata tacaacgtgg tgaaggcaaa gccgagtggc aaaaacggag tctgcgagga
    12781 cgcttcctga gaatcgtctt tattgcagta aatcacaggc aaatgcggaa acagctacgc
    12841 aaaacgcaac aactttgcgc aaaaagtgtg agcaagggct acgtcacatg gccgcgccgt
    12901 gtataataag ctcgtatgta ggctttattt cgctaatcac atacgaaaga tactcatggc
    12961 tcaaggcacg ctttatattg tttctgcccc cagtggcgcg ggtaaatcca gcctgattca
    13021 ggctttatta aaaacccaac cgttgtatga cacccaggtt tctgtttcac acaccacacg
    13081 ccaaccgcgt cctggtgaag tccacggtga acattatttc tttgttaatc atgatgaatt
    13141 taaagaaatg attagcagag atgcgttcct cgaacacgca gaagtttttg gtaattacta
    13201 tggcacttcg cgtgaggcca ttgagcaagt actggcgacc ggtgtcgatg tttttctcga
    13261 tatcgactgg cagggcgcgc agcaaattcg ccagaagatg ccgcacgcgc ggagtatctt
    13321 tattttaccg ccgtccaaaa ttgaactgga ccgccgtcta cgcggtcgcg gtcaggacag
    13381 cgaagaggtc attgcaaagc gtatggcgca agctgttgca gaaatgagcc attacgccga
    13441 atatgattat ctgattgtga atgatgactt cgataccgcg ttgaccgatt tgaagaccat
    13501 tattcgcgcc gaacgtctgc gcatgagccg ccaaaagcag cgtcatgacg ctttaatcag
    13561 caaattgttg gcagactgaa cctgatttca gtatcatgcc cagtcatttc ttcacctgtg
    13621 gagcttttta agtatggcac gcgtaactgt tcaggacgct gtagagaaaa ttggtaaccg
    13681 ttttgacctg gtactggtcg ccgcgcgtcg cgctcgtcag atgcaggtag gcggaaagga
    13741 tccgctggta ccggaagaaa acgataaaac cactgtaatc gcgctgcgcg aaatcgaaga
    13801 aggtctgatc aacaaccaga tcctcgacgt tcgcgaacgc caggaacagc aagagcagga
    13861 agccgctgaa ttacaagccg ttaccgctat tgctgaaggt cgtcgttaat cacaaagcgg
    13921 gtcgcccttg tatctgtttg aaagcctgaa tcaactgatt caaacctacc tgccggaaga
    13981 ccaaatcaag cgtctgcggc aggcgtatct cgttgcacgt gatgctcacg aggggcaaac
    14041 acgttcaagc ggtgaaccct atatcacgca cccggtagcg gttgcctgca ttctggccga
    14101 gatgaaactc gactatgaaa cgctgatggc ggcgctgctg catgacgtga ttgaagatac
    14161 tcccgccacc taccaggata tggaacagct ttttggtaaa agcgtcgccg agctggtaga
    14221 gggggtgtcg aaacttgata aactcaagtt ccgcgataag aaagaggcgc aggccgaaaa
    14281 ctttcgcaag atgattatgg cgatggtgca ggatatccgc gtcatcctca tcaaacttgc
    14341 cgaccgtacc cacaacatgc gcacgctggg ctcacttcgc ccggacaaac gtcgccgcat
    14401 cgcccgtgaa actctcgaaa tttacagccc gctggcgcac cgtttaggta tccaccacat
    14461 taaaaccgaa ctcgaagagc tgggttttga ggcgctgtat cccaaccgtt atcgcgtaat
    14521 caaagaagtg gtgaaagccg cgcgcggcaa ccgtaaagag atgatccaaa aaatcctctc
    14581 ggaaatcgaa gggcgtttgc aggaagcggg aattccctgc cgcgtcagtg gtcgcgagaa
    14641 gcatctttat tcgatttact gcaaaatggt gctcaaagag cagcgttttc actcaatcat
    14701 ggacatctac gctttccgcg tgatcgtcaa tgattccgac acctgttatc gcgtgctcgg
    14761 gcaaatgcat agcctgtaca agccgcgtcc gggtcgcgtg aaagactaca tcgccattcc
    14821 aaaagcgaac ggctatcagt ctttgcacac ctcgatgatc ggcccgcacg gtgtgccggt
    14881 tgaggtccag atccgtaccg aagatatgga tcagatggcg gagatgggtg ttgccgcgca
    14941 ctgggcttat aaagagcacg gcgaaaccag caccaccgcg caaatccgcg cccagcgctg
    15001 gatgcaaagc ctgctggagc tgcaacagag cgccggtagt tcgtttgaat ttatcgagag
    15061 cgttaaatcc gatctcttcc cggatgagat ttacgttttc acaccggaag ggcgaattgt
    15121 cgagctgcct gccggtgcaa cgcccgtcga cttcgcttat gcagtgcata ccgatatcgg
    15181 tcatgcctgc gtgggcgcac gcgttgaccg ccagccttac ccgctgtcgc agccgcttac
    15241 cagcggtcaa accgttgaaa tcattaccgc accgggcgct cgcccgaatg ccgcgtggct
    15301 gaactttgtc gttagctcaa aagcgcgcgc caaaattcgt cagttgctga aaaacctcaa
    15361 gcgtgatgat tctgtaagcc tgggccgtcg tctgctcaac catgctttgg gtggtagccg
    15421 taagctgaat gaaatcccgc aggaaaatat tcagcgcgag ctggatcgca tgaagctggc
    15481 aacgcttgac gatctgctgg cagaaatcgg acttggtaac gcaatgagcg tggtggtcgc
    15541 gaaaaatctg caacatgggg acgcctccat tccaccggca acccaaagcc acggacatct
    15601 gcccattaaa ggtgccgatg gcgtgctgat cacctttgcg aaatgctgcc gccctattcc
    15661 tggcgacccg attatcgccc acgtcagccc cggtaaaggt ctggtgatcc accatgaatc
    15721 ctgccgtaat atccgtggct accagaaaga gccagagaag tttatggctg tggaatggga
    15781 taaagagacg gcgcaggagt tcatcaccga aatcaaggtg gagatgttca atcatcaggg
    15841 cgcgctggca aacctgacgg cggcaattaa caccacgact tcgaatattc aaagtttgaa
    15901 tacggaagag aaagatggtc gcgtctacag cgcctttatt cgtctgaccg ctcgtgaccg
    15961 tgtgcatctg gcgaatatca tgcgcaaaat ccgcgtgatg ccagacgtga ttaaagtcac
    16021 ccgaaaccga aattaatgtt ttatgaaccc aacacgttat gcacgcatct gcgaaatgct
    16081 cgccaggcgg cagcctgatc tgaccgtctg catggagcag gtccacaaac ctcataacgt
    16141 ttctgcgatt attcgtaccg cagatgccgt tggcgtacat gaagttcacg ccgtctggcc
    16201 tggtagccgc atgcgcacca tggcttcggc agcggcgggt agtaacagct gggtacaggt
    16261 gaaaacacac cgcaccattg gcgatgccgt cgctcatctc aaaggccagg gcatgcagat
    16321 tctggcaacc catctttctg ataatgctgt cgatttccgc gaaattgatt acactcgccc
    16381 gacctgcatt ttgatgggac aggagaaaac gggcatcacg caggaagcat tggccctggc
    16441 ggatcaggac atcatcattc cgatgatcgg catggtgcag tcgctgaatg tttccgttgc
    16501 ctcagccctc attctttacg aagcccagcg tcagcggcaa aatgcaggca tgtacctgcg
    16561 tgaaaacagc atgttgccgg aagcagagca acaacgcctg ttgtttgaag gcggctatcc
    16621 ggtgctggcg aaagtcgcaa aacgcaaagg cctgccttat ccccacgtca atcagcaagg
    16681 cgagatcgaa gctgatgccg actggtgggc tactatgcag gctgcagggt aagtgccatg
    16741 aaaggtcgcc tgttagatgc tgtcccactc agttccctaa cgggcgttgg cgcagcactt
    16801 agtaacaaac tggcgaaaat caacctgcat accgtgcagg atctactctt acaccttccc
    16861 ctgcgctacg aagatcgcac ccatctctac cccatcggag aactactgcc gggcgtttat
    16921 gccacggtgg aaggcgaagt gctgaactgc aatatctcct tcggcggtcg gcggatgatg
    16981 acctgccaga tcagcgacgg ttccggcatc ctcaccatgc gctttttcaa tttcagcgcg
    17041 gcaatgaaaa atagcctggc gacgggccgc cgtgtactgg cttatggcga agcaaagcgc
    17101 ggtaaatatg gtgcggagat gatccacccg gaataccgcg tgcagggcga tctcagcacg
    17161 ccagaattac aggaaacgct cacgccggtt tatccaacaa cggaaggcgt aaagcaggcc
    17221 acgctgcgta aattaaccga ccaggcgctg gatctgctcg acacctgcgc cattgaagaa
    17281 ctcctgccgc cggaactgtc acaaggaatg atgacgctac cggaagcgtt gcgcactttg
    17341 caccgcccgc caccgacgct acagcttagc gatctggaaa ccgggcagca tccggcgcaa
    17401 cgtcgtctga ttctggaaga actgctggcg cacaacctca gcatgttagc cttacgtgcc
    17461 ggagcacagc gttttcatgc ccagccgctg agcgccaatg acacgctgaa aaataaactc
    17521 ctcgccgcct taccgttcaa gccaacgggc gcacaggcac gcgtagtggc ggagatcgag
    17581 catgatatgg cgctggatgt gccgatgatg cgcctggtac agggcgatgt aggttccggt
    17641 aaaacgctgg tcgccgccct cgccgcgctg cgtgcgattg cccacggcaa acaggtggca
    17701 ctgatggcac caaccgaatt actcgccgag cagcacgcca ataacttccg caactggttt
    17761 gcaccgctcg gtatcgaagt gggctggctc gccggtaagc agaaaggcaa agcgcgactg
    17821 tctcagcagg aagccatcgc cagcggtcag gtgcagatga ttgtcggtac acacgccatc
    17881 ttccaggaac aggtgcagtt taacggcctg gcgctggtga ttatcgacga acagcatcgt
    17941 tttggcgtgc atcagcgtct ggcattgtgg gagaaaggcc agcagcaggg cttccatccg
    18001 catcagttga tcatgaccgc cacgccgatc ccccgcacgc tggcaatgac ggcgtatgcc
    18061 gatctcgata cctcggtgat agatgagctg ccgccaggcc gcacgccagt gactacggtc
    18121 gctattcctg atacccgccg taccgacatc attgaccgcg tgcgccacgc ctgcataact
    18181 gaaggtcgtc aggcgtactg ggtttgtacg ttgattgaag agtcagagtt actggaagcg
    18241 caggcggcgg aagctacctg ggaagagttg aaactggcgc taccagaatt gaatgttggc
    18301 ctggtacacg ggcggatgaa accagccgag aaacaggcgg tgatggcgtc gtttaaacaa
    18361 ggtgagctac atctgctggt tgccactacc gttattgaag tcggtgttga tgtgcctaac
    18421 gccagcctga tgattatcga aaaccctgag cgtctgggtc tggcgcagtt acaccagctg
    18481 cgtgggcgcg taggtcgtgg cgcggtggct tctcactgcg tgctgctcta caaaacgccg
    18541 ctttctaaaa cggcgcaaat tcgcctgcaa gtgctgcgcg acagtaacga cggttttgtg
    18601 attgcgcaaa aagatctgga gattcgcggc cctggcgaat tgttaggcac gcgtcagacg
    18661 ggtaatgctg aatttaaagt ggcggattta ctgcgcgatc aggcgatgat cccggaagtt
    18721 cagcgcctgg cgcgacatat tcacgaacgt tacccacaac aggcaaaagc cttgatagaa
    18781 cgctggatgc cggagacaga acgttactcg aatgcgtaaa aggcggcggt gagaagaccg
    18841 ccgcttcatc ggttaaccgg caaaaatcgg caacattaaa tacagcttaa tcaccagcgc
    18901 attgacgata tcgataaaga acgcaccgac catcggcacc accaaaaacg ccatgtgcga
    18961 cgggccaaag cgttcagtga tcgcctgcat gttggcgatt gccgttggcg ttgcaccgag
    19021 gccaaaacca cagtgacccg cagccagcac tgccgcatcg tagtttttgc ccatcatgcg
    19081 ccaggtaacg aagatggcat acaacgccat gaagatggtc tgtaccacca gaatcgccag
    19141 catcggcagt gccagcgaag ccagctccca cagtttcagc cccatcaacg ccatcgccag
    19201 gaacaacgac aagcttacgt tacccagcac ggataccgca cgctcaaaga cgcggtaaaa
    19261 gcccattatt gacagaccgt tgctcagaat cacgccaaca aacagtacac agacgaaggt
    19321 cggcagttca aaagcagtgc cagccaaaag ttgcgcaaca attttcccca ccgtcaggca
    19381 gatagcaatc agcgcgatag tttcaatcag caccaacgag gtgatcatgc gtcccacatc
    19441 cggcttttca aacgccgtcg ggacttcctg gtcatccgga ataccgttcg gcgtggtgga
    19501 gtgtttcacc agatagcgcg ccaccggacc gccaatcaag ccgcccagca ccagaccgaa
    19561 cgttgcacag gccatagcca cttccgtcgc attggtgaag ccataacgtt caatgaacaa
    19621 tttactccac gcagcgcccg taccgtgacc gccggaaagc gtaatagaac cggccaacag
    19681 ccccatcagc ggatcaagcc ctaacaggct agccatacca atgccaatgg cattttgcat
    19741 caccaacaga ccaacaacca caatcaagaa gatgccaacc acacgcccac cggcacgcaa
    19801 actggcaatg ttggcgttca ggccaatggt ggcgaagaaa gccagcatta acggatcgcg
    19861 cagggacata tcaaagttga cttcccagcc catgcttttt ttcagtacta gtagcgccag
    19921 cgccaccaac aaaccacccg caacaggttc cggtatggtg tatttcttca aaaaggagac
    19981 ggaatggacc aacttacgcc cgagcagcaa cgtcagcgtt gcggcaacaa gcgttgctaa
    20041 agtatcgaga tgaaacatag ttactccttt gtatccgcat cactctttca tactcgtcat
    20101 acttcatcgc gcagtggcaa cttcatgaac tatgacgggt atcgagttat gctttcctgg
    20161 gtcaaattct cagctgttaa tgaacaaatg gcatggattt taagcagaaa aataccaaaa
    20221 gttatataaa aaagtgcatt tatctcattt aaatatttag atgaggatgt ggcaaacgtt
    20281 tgcttttctc tttcggtcag ctaaaatgcc cgctttgcta ccacgggatt gttttcaatg
    20341 tctgtttcca ccctcgagtc agaaaatgcg caaccggttg cgcagactca aaacagcgaa
    20401 ctgatttacc gtcttgaaga tcgtccgccg cttcctcaaa ccctgtttgc cgcctgtcag
    20461 catctgctgg cgatgttcgt tgcggtgatc acgccagcgc tattaatctg ccaggcgctg
    20521 ggtttaccgg cacaagacac gcaacacatt attagtatgt cgctgtttgc ctccggtgtg
    20581 gcatcgatta ttcaaattaa ggcctggggt ccggttggct ccgggctgtt gtctattcag
    20641 ggcaccagct tcaactttgt tgccccgctg attatgggcg gtaccgcgct gaaaaccggt
    20701 ggtgctgatg ttcctaccat gatggcggct ttgttcggca cgttgatgct ggcaagttgc
    20761 accgagatgg tgatctcccg cgttctgcat ctggcgcgcc gcattattac gccgctggtt
    20821 tctggcgttg tggtgatgat tatcggcctg tcgctaattc aggtggggct gacctccatt
    20881 ggcggcggtt acgcagccat gagcgataac accttcggcg caccgaaaaa tctgctgctg
    20941 gcaggcgtgg ttttagcctt aattatcctg cttaaccgtc aacgtaaccc ttacttacgc
    21001 gtggcctcac tggtaattgc gatggcggcc ggatatgcgc tggcgtggtt tatgggcatg
    21061 ttgccagaaa gcaacgaacc gatgacgcaa gaactgatta tggtgccaac gccgctctat
    21121 tacggtcttg gcattgaatg gagtctgctg ctgccgctga tgctggtctt tatgatcact
    21181 tcgctggaaa ccattggcga tatcacggcg acctctgacg tttccgaaca gccagtgtcc
    21241 ggtccgctgt acatgaaacg cctgaaaggc ggcgtgctgg caaacggcct gaactcgttt
    21301 gtttcggcgg tgtttaacac cttcccgaac tcctgcttcg ggcaaaacaa cggagtgatc
    21361 cagttgactg gtgttgccag ccgctatgtc ggttttgtcg tcgcgctgat gttgatcgtg
    21421 ctgggtctgt tcccggcagt gagcgatttt gtacaacaca ttccagaacc ggttctgggc
    21481 ggcgcaacgc ttgtaatgtt tggcaccatc gccgcctccg gtgtgcgtat cgtttctcgt
    21541 gagccgctga accgtcgggc gattctgatt atcgcgttgt cgctggcggt tggtctgggc
    21601 gtgtctcagc agccgctgat tttgcagttt gcccctgaat ggctgaaaaa cctgctctcc
    21661 tccgggatcg ccgcgggcgg tattactgcc atcgtgctga atctgatttt cccaccagaa
    21721 aaacagtaat tcactggcgg cggtaagata attaccgccg cagtctcacc tctttcacca
    21781 ttcccgcctt gaggattgcg cgtaaatcgt gcataactcc cttatgtgca tttcacggga
    21841 tggaagacca tgaaatttat tgggaagctg cttctctaca ttctcatcgc tctgttagtg
    21901 gtgatcgctg gcctctattt tcttctgcaa acccgctggg gagcagaaca tatcagcgca
    21961 tgggtttccg agaatagcga ctatcatctg gccttcgggg cgatggatca ccgtttttcc
    22021 gcgccatctc atatcgtgct ggagaatgtc acgtttggtc gtgatggtca gcccgcgccc
    22081 ctggtggcaa aaagtgtcga cattgcgcta agcagtcgcc aactgaccga accacgccat
    22141 gtcgatacca tcctgctgga aaacgggacg ctgaatctca ccgaccagac cgcgccgcta
    22201 ccgttcaaag ccgatcgtct gcaactgcgt gatatggcgt ttaatagccc gaatagcgaa
    22261 tggaaactga gcgcgcagcg ggtaaatggc ggcgtggttc cgtggtcacc agaagccggt
    22321 aaagtgctgg gtacgaaggc gcagattcag tttagtgccg gatcgctttc gctcaatgat
    22381 gttcctgcca ccaatgtact gattgaaggc agtattgata acgatcgcgt tacgctgact
    22441 aacctgggtg ccgacatcgc ccgcgggaca ttaaccggaa acgcgcagcg taacgccgac
    22501 ggcagctggc aagtggaaaa tctgcgcatg gcggatatcc gtctacaaag cgaaaaatcg
    22561 ctaaccgact tctttgcgcc attacgctct gtcccgtcgt tgcagattgg tcgcttggaa
    22621 gtcatcgatg ctcgtctgca aggtccggac tgggcggtga ccgacctcga tctcagcttg
    22681 cgcaacatga ccttcagtaa agatgactgg cagacacagg aaggcaaact gtcgatgaac
    22741 gccagcgagt tcatttatgg ttcgctgcat ttatttgacc cgattataaa cgcggaattt
    22801 tccccgcagg gcgtagcgct gcgccagttc accagccgct gggaaggggg tatggtcaga
    22861 acgtcaggga actggctgcg tgacggtaaa acgttgatcc ttgatgatgc ggcaattgcc
    22921 gggctggaat ataccttgcc gaaaaactgg caacagttgt ggatggaaac gacacccggt
    22981 tggttaaaca gcctgcaact gaagagattt agcgccagcc gcaatctgat cattgatatc
    23041 gaccctgact tcccgtggca gctcaccgcg ctcgatggtt acggtgccaa cctgacgctg
    23101 gttaccgatc ataaatgggg cgtctggagt ggctcggcga atctgaatgc cgccgccgcg
    23161 acattcaatc gtgttgatgt tcgtcgcccg tcgctggcgc tgaccgccaa cagcagcacg
    23221 gtgaatatca gcgaactgag tgcatttact gaaaaaggca ttctggaagc caccgcaagt
    23281 gtttcacaaa cgccacaacg tcagacccat atcagcctga atggacgcgg tgtgccggtg
    23341 aatattttgc aacaatgggg atggcctgaa ttacagttga ctggcgacgg caatattcag
    23401 cttaccgcca gtggagatat tcaggccaat gccccgctga aacctacggt tagcgggcaa
    23461 ttgcatgccg tgaacgccgc aaagcagcaa gtgactcaaa ccatgaatgc gggcgtcgtt
    23521 tccagcggtg aagttacatc gacggagccg gtgcggtaaa catcaatctc gccgggtacg
    23581 actcagacgt acccggcatt ccgtcaatag attacaacgt aattgtcagc gcattccctt
    23641 gaggcgtcac caccagcccc tgctcacttt cagcctgcga accgccttgc agaccattta
    23701 ctttcacaac attgcgcaag cacagcgtcc agttcttcgc ctcgcccgca ccagtcacag
    23761 taatcgtgtt gccagtacgt gctgctttta aagtaaagat caccgatccg tcagcagcag
    23821 gcacttcaca gacggcttca tgcccgtctt gcagattaaa gaggtggaat gccgtgcctt
    23881 cgtgccacac gtaatcggga cgttgatcgt tgttgcccag cgccagtaga gtgttatcac
    23941 gcacataaac gggcagactc aggaagccgt gctgctgttt atgccagcga ctaccgtcga
    24001 gttcatcgtt gtgccacagg tgtgtccagc gaccttccgg caggtagaac tgcacatcgc
    24061 ccgcttcagt gaacaccggc gcaaccatca cgttgtcgcc taacatgtat tgacggtcaa
    24121 ggtaatcaca agccggatcg tccgggaact ccatcatcat ggcccgcatc atcggcgtac
    24181 cccgcgcgtt cgcacgcgca gcttcacgat acagatacgg catcatgcgg catttcagtt
    24241 gcgtgaagaa gcgcaccaca tcacaggact catcatcgta ggcccacggc acacgataag
    24301 atttgctacc gtgtaaacgg ctatggctgg agagcaaacc aaacgcgcac cagcgtttgt
    24361 aaacgtgcgc cggagcggta ttttcaaagc cgccgatatc gtggctccag aagccaaaac
    24421 ctgaaaggcc aatagacaaa ccaccgcgca ggctttccgc cattgattcg tagttagcgt
    24481 aacaatcgcc accccagtgt accgggaatt tctgcgcacc gacggaggcc gagcgggcaa
    24541 acaagacagc ttcttcctca ccaacggtgt ccttgagcac gttccacacc agttcgttgt
    24601 agatgtacgc ataatggtta tgcattttct gcggatcgga accgtcaaac cactgaacat
    24661 cagttgggat acgttcgcca aagtcggtct taaagcaatc aacgcccatc gcgaccagac
    24721 ctttcagttt gtcggcgtac catttgcagg catccggatt ggtaaagtca taaatcgcca
    24781 gacctggctg ccatttatcc cactgccata gcgaaccgtc cgggcgtttg agtaaatagc
    24841 ctttctcttg taactcttta aagacggggg atttttgacc gatatagggg ttaatccaga
    24901 cgcagatttt cagccctttc gctttcaggc ggcggatcat cccttccggg tcagggaaag
    24961 tcagcgggtc ccactcaaaa tcgcaccact ggaaggcttt catccagaaa cagtcaaagt
    25021 ggaaaacatg cagcggcaga ttgcgttccg ccataccatc gataaagctg tttaccgtcg
    25081 cttcgtcgta gttggtggta aatgaagtgg ttagccacag gccgaaggac cacgggggcg
    25141 gcagcgccgg acgaccagta aagcgggtat aacgatcaag taccgctttc ggcgtcgggc
    25201 cgtcgataac aaagtattcg agatattcac tctcaacgct gaactgcact ttggagactt
    25261 tctccgatcc cacttcaaaa gagacacact ggggatgatt gaccagtacc ccataaccac
    25321 ggttagtcat gtagaacggg atatttttat acgcctgttc agtacttgtg ccgccgtccc
    25381 ggttccaggt ctctaccgtc tggccattgc gcaccagggc agtaaagcgc tctcccagac
    25441 cgtaaactgt ttcgccaacg ccaagatcaa gccgctcaaa catataattg cgttgattat
    25501 tcgtgtcctg cacgtagcca ttatttttca cctgactacc ggtaatacgt tcgccgttgc
    25561 gcagaaaatc cagtgaccag aactcacctt tgctgacacg cgcgcttaag ttgccacttt
    25621 taaactcagc ataacgttct gtgttttcga ttgtgacctt cacgtcctgc aaaatattga
    25681 gcggataatg aggaccgtta ttcagcgccc cctgaaaata ctcaatccgc acaccgacaa
    25741 taccttcctg tggggagaaa aagcgcagcg taaataaagg cgtatcaagc tgccaggtac
    25801 gttcacgcac atcacggggg gcagcataga ccaccatttc attatcctgc tgttcaacct
    25861 cgaatacctg aagcgggtga atcaaattga gcccaggttg aatcaaccag tttccatcgc
    25921 taattttcat tgccgttcct tagttctgca cttcttgaga ggtgaattgt tgctggcaac
    25981 ggcgtttacc ctgagccagc tgttccataa cggttttcag attgtgcgtc gtgagtgagt
    26041 agtagcgttt agcgataatc gcgctcagca aataacagat cgccggaaca atcgtgaata
    26101 gcgcaataat gatgctaatc gtggcgctgt tctgcgcttt ttctgccgca tcatatccgc
    26161 cataagccag catccagccg ataagagcgc cgccgaaggc caaccccagt ttgagcacaa
    26221 acagcgtgcc agcaaaactg atcccggtca ggcgtttacc attgcaccac tcgccgtagt
    26281 cgacggtatc gaacatcatt acccactgga taggtgtcac cagttgatgc aacacaccaa
    26341 tcacgaagat gaagacaaac atagtgatgc tggcctgcat gggaacaaag aacatcgcga
    26401 ggctaatcac tgccagcagg gcgttcgtcc accagaagat agtgacttta catttccagt
    26461 cggtcagagg ttttgccagt gcggaaccaa tcaggttacc cacgcaataa gtggtgagaa
    26521 aagcgacaaa cacttccggc gtgcccaaaa tccatgtgac gtaatacatc atcgccccac
    26581 cgcgtacgca caccgccagg atattgaaaa tggttagtaa accgacaatc cgccactggt
    26641 cgttttgcca gatatcacgt aaatcttccc gcatagacgt tgttgtaggt ggtgcttcaa
    26701 cgcgttcttt agtggtgaag aaacaaaatg ccagcatcat taatgccacc acggaaagga
    26761 ccacgatacc gccctggaaa ccgagtggtt tattatcacc gccaattaaa ttaaccagtg
    26821 gcatcatcag aacagtagaa agcatgcctc ccgcggtcgc cagcacaaaa cgccaggatt
    26881 gcagcgagat acgctgagtc gggtcattgg tgattacacc acccaatgcg cagtaaggga
    26941 tattgacgac ggtataaagt aaggtaagtt agggtgtaag taattgctgc atagatcatt
    27001 ttgccgttca tactgagatc tggcgtgcta taggccagta cacagacgat cccgaatggc
    27061 agtgcgccaa acagtaccca cggacgaaat ttaccccagc gagagcgcgt tcgatcggcc
    27121 aacaacccca tgcaaggatc ggaaatcgca tccagtgcac gagcgaccaa aaacatggtt
    27181 ccgacaaatc cggcaggaat gccaaaaata tcggtataaa agaacatcat atataacatt
    27241 acgttatcga aaataatgtg gctggcggcg tctcccatgc cataaccaat tttctcttta
    27301 acggacaaca cttcactctt catctgcttt tccttgatgc tataggggtg ctgagaccga
    27361 taacgcattt tgtaaaccat ccctgtgaga gcgaatattc cgatatctgg ttattaattt
    27421 atgattcttg ttttatgtga tcgtggtagc gttaattccg ctcatatatc attgtaaaat
    27481 atgggtttta tataaattat aatgctttcg tgataatacg ctgcgtttat taggcggaaa
    27541 aaactgatct gggggatgta gaaactcaag gaagtagcta taatgcgccc cgcctccatg
    27601 tagcaatcga ggcgcggaag atcgtcgtct ccggtgaggc ggctggactt caaatccagt
    27661 tggggccgcc agcggtcccg ggcaggttcg actcctgtga tcttccgcca gtttacctct
    27721 tctgaactct cccaatgtca ataaatctct tctaatccct tgtatatcag tatttatttg
    27781 tatcctgagg tcaacctact tcaccccgta tcaagtttct tttgggggta ctttaggggg
    27841 tgttatttcc tattgaaata caagcacccc cataatagga aaaatgtgat acataacaac
    27901 ggcttttaac aatatctttg ttatacaagg ggtgtattgg gggtatcagg ttagttactg
    27961 gcagaaatcc ccctcaggaa catgaattca tcaaaagccg gaggatgtca tggcattaac
    28021 cgatatcaaa gtgaaaaccg caaaacccaa agacaaaccc tataagcttg ctgatggcgg
    28081 cggtatgtat ctactgatta acactaatgg ttctaaatac tggcgcatga agtaccgctt
    28141 tgctggtaaa gaaaagatgc tttctattgg cgtatacccc gatgtaacgc tagccgatgc
    28201 tcgcgagaaa cgcagtgaag ccaggaaact cctcgctgct ggaggcgatc caggggaggc
    28261 aaaaaaggaa gaaaaaatcg cgcagcaaat gagtctgaaa aatacgttcg aggctattgc
    28321 tcgcgaatgg caccagttaa aagctgatcg gtggtcatta cgctatcgtg atgaaatcat
    28381 tgataccttt gaaaaagaca tttttcccta cattggcaag cgccctattg ccgaaatcaa
    28441 accaatggag ttactggaag cactgcgtaa aatggaaaaa cgtggagctc tggagaaaat
    28501 gcgcaaggtc cgccagcgct gtggtgaggt gttccgctat gcaattgtta ctggccgggc
    28561 agactataac ccagcccccg atcttgccag tgctttagct acaccgaaaa aagtacattt
    28621 tccctttctt actgctaatg aacttcccca cttcctcaac gatctggcgg gttataccgg
    28681 aagtataatc actaagacag ctactcagat cattatgtta accggcgtac gaacacaaga
    28741 actgcgtttt gcacgctggg aagatatcga ttttgagaca aaattgtggg agatcccggc
    28801 agaagtaatg aagatgaagc gacctcatat tgtgccgctc tctgagcagg tcattatgct
    28861 atttaagcag cttgaaccaa tctcaaaaca tcatccccta gtctttatcg gtagaaatga
    28921 tccccgcaaa ccaattagta aggaaagcat taaccaagta attgaattac ttggatataa
    28981 gggaagactc acagggcacg gtttcagaca caccatgagt acaattctgc atgaacaagg
    29041 ctttaattcc gccttgattg aaatgcagtt agctcatgtg gataaaaatt ccatcagggg
    29101 tacctataat catgccctgt atctcgatgg ccgccgtgaa atgatgcaat ggtacgctga
    29161 ttacattgat tcactctcca gccgggaaag ttaagtcaga aatacttttc cctgatgttt
    29221 tcctcaatta agtattttat cctctggcct acaaggctgg aggatttttg ttattatatt
    29281 atttttttgc gtcttcaaac ttattacaaa aacataatta aaaacaaatt gacattttta
    29341 aattggcaat tacaaaatac agccttgcag ttatttcgat acctttagca aaggaaaagt
    29401 tgattcagat ataatttagc atttcaatca tctgacatca ttataaaaaa tgagattcac
    29461 ataaagataa aattattgtt ctcaaggaaa gcatgaaggg gcttcgtaag caatattcat
    29521 tcggtgccaa gacaggactt gatacacaga atctcaactt ttatgctctc agactatatc
    29581 accacatgtt ttacaggctc agaaaatgaa cgatagatta tgctttgaag tgcacgataa
    29641 caagggttat ttcgtatttc cggatacctg gtttggccca ttgctggggg agtttgaaga
    29701 agtgcttgat gcatacgacg ctgatgaaat cagcgagacg agttacatca acaaactccg
    29761 acgccttgct caacgggagc cggactttat tgatatacac gctcatctgg catatgcatt
    29821 tctggaacag aatgccccgc gtaaggcact gaacgccgct cttaaaggac tggcggcagg
    29881 taatcgtatc atcccggaaa gtttctgtgg tgaaataatc tggatgcatc ccgaaaaccg
    29941 gccttatctg cgcgcgcttt atgcagcaat tctcgcaaat gtgcacttac agcgccatca
    30001 ggatgcggtt atgctcactg ataaaatact ggcatacaat cctgaagata atcagggggc
    30061 tcgctggttg ttgggttcag agctgttacg caccggcgat catgagcgag ccttcagtgt
    30121 actgaaagag catgctgatg aattttcacc ttactggtat gaactcggtc tcctgcattt
    30181 tctgaacggg gaacatgtta aggccgccac agcttttcgc catggctttg ccacaaacac
    30241 ttacatcgca gaaatgctgt gcggaaatct gcatccattt ccacttgctg tgtggcatga
    30301 cttctccggc tctctggata cggcagaaga ttactatgca acatactcac cattatgggg
    30361 acaatatccg gaagcattgc tgtttgtaaa ctggctctat aaccattcct ccgtgctgca
    30421 tgaacgatca gaaatcatta aatgcgccga aatgctgatt caggaagatg atttcgaaat
    30481 atgcgaaagt attttaagac agcaggagca cctgtggaaa agaattgaca agacactttc
    30541 tgaggaaatt gtacagaaat gcagaaatat gaacggtgaa tatatctggc cctggatatt
    30601 gcccttttcc gcagcaggga tgaaacattc aagaatccag tatcagtaga tattgcatta
    30661 gtgtaacttt aaaacaagta ataatttatt atccaataat aacactgtgt tccttcccca
    30721 ggtgattacc ctggggttat tttctgaaag tttcctttgc aaaaggatag cgttgtaaat
    30781 tactttaacg aattactcca tgaactcatt aaagttcttt cgtaatactt tatcgtcaaa
    30841 cacgttatag ctggcatcaa aaaccacaga tttaccctgc cagcgggtaa tcccctcctc
    30901 tggcgttttc ggttgaactg gtatccccgt ccaacacagg cagccattat aaacagtaaa
    30961 aaattgtatt cgctgttatg aaatcaatag tcctgacgta ctgttgaatg tgcttgtgtt
    31021 ctgaacctcg tacaaccagt acatttgata tcgaattata aacaaaacat tgcatccaac
    31081 ttttttaata tggacaatag ttgtccatac ggtggtttta tattcgataa atcaatggat
    31141 ttcattgagt cacaggactg gtaatggatg aaaatgcttt aaggtttgcc tcatactggc
    31201 gcaactcgct tgcagatgct gagtcaggaa agggctgttt tgaacggaaa gacgcccaaa
    31261 atttcactca ctggcatggg atagcggcgg gacgtcttga cgaagcgatt gtcaataaat
    31321 tttttaaggg agaaaaagac gatgtcgaaa ctgtcaatgt catcttacgc ccaaaagttt
    31381 atttccggtt actgcagcat ggtaaggacc gttccgcagg cacgcctgat attgttacac
    31441 cgatagtgac gccagccttg ctaagccgtg agggtttttt atatccgacg ccagcgacct
    31501 ccattcccag agacctgctt gaacctttgc caaaaggagc attttcgatt ggtgagattg
    31561 ggcagtatga caaatacaag acgacccata ctgatcctac ccacgtaatg tggacacggc
    31621 cctaagcgag attatggttt tcaaattgtt ccgggctgag accgccacag gcactgtgac
    31681 gacgccagcg attgtaatca cactcgatat aattaaacac cgttgcccgc attatttccc
    31741 ggctgctaaa gcgttccccg tggatacatt ccaccttcag cgaatgaaag aagctttcca
    31801 cacaggcatt gtcataacag tagcctttcg cactcatact gccacgcagg ttgtgtcgct
    31861 tcagcattgc ctgataatcc cctgaacagt attgaccacc acggtccgta tgaacaatga
    31921 cgctttccgg gcgtcttctc cgccacaacg ccatttgcag tgcatcacag gccagttgtg
    31981 ctgtcattcg cggtgacatc gaccagccaa tgacggcgcg tgaccacagg tcgatgacta
    32041 ctgcgagata caaccagccc tcatcggtac gcaagtacgt gatgtcaccc gcccacttct
    32101 ggtttcgggc cactggcgct gaagtcctgc tccagcagat tctccaatac gggcaggcca
    32161 tgtgcacggt agctgaccgg gctgaacttc cggctggctt tcgcccgcag cccctgacga
    32221 cgcaggctgg cggcaatggt tttaatattg aactccggca gttcgtcagc aaggcgggga
    32281 gcaccgtatc gctgctttgc ctcaaagaat gccttatgga cagcggcatc gcaggtgagc
    32341 cgaaactgtt ggcgcaggct catctggtga cgacgcctga gccagacata ccagccgctg
    32401 cgggcaaccc gaagtacacg acacatcgct ttgatgctga actctgcccg atgattttcg
    32461 ataaagacat acttcatttc aggcgcttcg cgaagtatgt cgcggccttt tggaggatgg
    32521 acagttcctc agcctgctcc gccagttgtc gtttaaggcg gacattttca gcggccagtt
    32581 cgctttcgcg ctctgacgaa ctcatttgtt gctgctgttt actgcgccag gcataaagct
    32641 gagattcata caggctgagt tcacgggctg cggcggccac accgatgcgt tcagcgagtt
    32701 tcagggcttc gttacgaaat tcaggcgtat gttgtttacg gggcttcttg ctgattgata
    32761 ctggttttgt catgagtcac ctctggttga gagtttactc acttagtcct gtgtccacta
    32821 ttggtgggta agatcaccct ccattaaaac taaaacgggc gctttctgat atatcatctg
    32881 taagcgcagt aataccatta gtagcacctc tcggcggcgc attgattatt gaatattata
    32941 actggaatgt tatatttctt gttatgatgc tgttcagtat tataataatg ttattatcac
    33001 caaagataac tcatgcaaat gagaaagtaa tatgtcaaaa cagtgcatcc tttgtgacca
    33061 aagagtttat ctgcggcact atacttgtct cattaatact cagttctcta ttttgctaca
    33121 tatcaatatc accatcaatt ttcatgcata cttatggcct gagtactttt agttactcaa
    33181 ttattttctc cttctcagtt ttatttttca taatcggaaa tcaattatca aagattgaaa
    33241 aattatccaa cctatggttt ttattaccca a
//
LOCUS       DADMGC010000029        33129 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.029, whole genome shotgun sequence.
ACCESSION   DADMGC010000029 DADMGC010000000
VERSION     DADMGC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.029"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..224
                     /locus_tag="JNZ73_001373"
     CDS             <1..224
                     /locus_tag="JNZ73_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY8731701.1"
                     /translation="PQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTVL
                     DGKVIDERTTSHINRMQEDTLISSAYPEE"
     gene            398..1167
                     /locus_tag="JNZ73_001374"
                     /pseudo
     CDS             398..1167
                     /locus_tag="JNZ73_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1200..2720)
                     /locus_tag="JNZ73_001375"
     CDS             complement(1200..2720)
                     /locus_tag="JNZ73_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8731702.1"
                     /translation="MRTVITYLRFSSATQGAEGADSTRRQNELFKQWLRKNSDAQVVA
                     SFNDEGLSGYKGKHLSGQFGDMLSRIESGEFPEGTILLVEAIDRIGRLEHLETEALMN
                     RIIAHGIEIHTLQDGLIYTRDALSDDLGISIIQRVKSYIAHQESKQKSFRVSQKWEQR
                     AKLALAGEQRLTRNVPGWIDPETFKLNERAETVRLIFKLLLDGESLHNIARHLQANNI
                     SSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEAAIDAATFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDTMKRKASLTDKELPQLKDIDLTTKAGRVECQLI
                     LSKHLKGLTLGKGSVTITLQNDTEITIPTDPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            2811..3530
                     /gene="tonB"
                     /locus_tag="JNZ73_001376"
     CDS             2811..3530
                     /gene="tonB"
                     /locus_tag="JNZ73_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HAY8731703.1"
                     /translation="MPLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(3570..3968)
                     /gene="yciA"
                     /locus_tag="JNZ73_001377"
     CDS             complement(3570..3968)
                     /gene="yciA"
                     /locus_tag="JNZ73_001377"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HAY8731704.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4073..4612)
                     /locus_tag="JNZ73_001378"
     CDS             complement(4073..4612)
                     /locus_tag="JNZ73_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HAY8731705.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAVALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(4642..5385)
                     /locus_tag="JNZ73_001379"
     CDS             complement(4642..5385)
                     /locus_tag="JNZ73_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HAY8731706.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVCALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(5413..5502)
                     /locus_tag="JNZ73_001380"
                     /pseudo
     CDS             complement(5413..5502)
                     /locus_tag="JNZ73_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            5742..6380
                     /gene="ompW"
                     /locus_tag="JNZ73_001381"
     CDS             5742..6380
                     /gene="ompW"
                     /locus_tag="JNZ73_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HAY8731707.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGS
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(6440..6946)
                     /locus_tag="JNZ73_001382"
     CDS             complement(6440..6946)
                     /locus_tag="JNZ73_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY8731708.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDCVEAKK"
     gene            complement(6992..7492)
                     /locus_tag="JNZ73_001383"
     CDS             complement(6992..7492)
                     /locus_tag="JNZ73_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY8731709.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLPRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESKSNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(7578..7757)
                     /locus_tag="JNZ73_001384"
     CDS             complement(7578..7757)
                     /locus_tag="JNZ73_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAY8731710.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSDKS"
     gene            complement(8138..8944)
                     /gene="trpA"
                     /locus_tag="JNZ73_001385"
     CDS             complement(8138..8944)
                     /gene="trpA"
                     /locus_tag="JNZ73_001385"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HAY8731711.1"
                     /translation="MERYESLFAQLKERKEGAFIPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(8944..10137)
                     /gene="trpB"
                     /locus_tag="JNZ73_001386"
     CDS             complement(8944..10137)
                     /gene="trpB"
                     /locus_tag="JNZ73_001386"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HAY8731712.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(10149..11507)
                     /gene="trpCF"
                     /locus_tag="JNZ73_001387"
     CDS             complement(10149..11507)
                     /gene="trpCF"
                     /locus_tag="JNZ73_001387"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HAY8731713.1"
                     /translation="MQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLLI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(11511..13106)
                     /gene="trpD"
                     /locus_tag="JNZ73_001388"
     CDS             complement(11511..13106)
                     /gene="trpD"
                     /locus_tag="JNZ73_001388"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HAY8731714.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(13106..14668)
                     /gene="trpE"
                     /locus_tag="JNZ73_001389"
     CDS             complement(13106..14668)
                     /gene="trpE"
                     /locus_tag="JNZ73_001389"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HAY8731715.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDNALHITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            14941..15822
                     /gene="yciV"
                     /locus_tag="JNZ73_001390"
     CDS             14941..15822
                     /gene="yciV"
                     /locus_tag="JNZ73_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HAY8731716.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            15819..16439
                     /locus_tag="JNZ73_001391"
     CDS             15819..16439
                     /locus_tag="JNZ73_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAY8731717.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            16540..17412
                     /gene="rluB"
                     /locus_tag="JNZ73_001392"
     CDS             16540..17412
                     /gene="rluB"
                     /locus_tag="JNZ73_001392"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HAY8731718.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(17452..18042)
                     /gene="cobO"
                     /locus_tag="JNZ73_001393"
     CDS             complement(17452..18042)
                     /gene="cobO"
                     /locus_tag="JNZ73_001393"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAY8731719.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(18039..18797)
                     /locus_tag="JNZ73_001394"
     CDS             complement(18039..18797)
                     /locus_tag="JNZ73_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HAY8731720.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            19017..20066
                     /gene="sohB"
                     /locus_tag="JNZ73_001395"
     CDS             19017..20066
                     /gene="sohB"
                     /locus_tag="JNZ73_001395"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HAY8731721.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKETKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(20102..20353)
                     /locus_tag="JNZ73_001396"
     CDS             complement(20102..20353)
                     /locus_tag="JNZ73_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HAY8731722.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            20733..23330
                     /gene="topA"
                     /locus_tag="JNZ73_001397"
     CDS             20733..23330
                     /gene="topA"
                     /locus_tag="JNZ73_001397"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAY8731723.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPSKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            23540..24514
                     /gene="cysB"
                     /locus_tag="JNZ73_001398"
     CDS             23540..24514
                     /gene="cysB"
                     /locus_tag="JNZ73_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HAY8731724.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            24809..24973
                     /locus_tag="JNZ73_001399"
     CDS             24809..24973
                     /locus_tag="JNZ73_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HAY8731725.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLTVFLGSALFWVV
                     VALLIWKVWG"
     gene            24976..25143
                     /locus_tag="JNZ73_001400"
     CDS             24976..25143
                     /locus_tag="JNZ73_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731726.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            25257..25352
                     /locus_tag="JNZ73_001401"
     CDS             25257..25352
                     /locus_tag="JNZ73_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731727.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            25516..28191
                     /gene="acnA"
                     /locus_tag="JNZ73_001402"
     CDS             25516..28191
                     /gene="acnA"
                     /locus_tag="JNZ73_001402"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HAY8731728.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(28255..28845)
                     /gene="ribA"
                     /locus_tag="JNZ73_001403"
     CDS             complement(28255..28845)
                     /gene="ribA"
                     /locus_tag="JNZ73_001403"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HAY8731729.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYADISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            29015..29779
                     /gene="pgpB"
                     /locus_tag="JNZ73_001404"
     CDS             29015..29779
                     /gene="pgpB"
                     /locus_tag="JNZ73_001404"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HAY8731730.1"
                     /translation="MRSIARRTAVGAALLFVMPVAVWISGWRWQPGEQSWRLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFSILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWTLVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            29928..30236
                     /gene="lapA"
                     /locus_tag="JNZ73_001405"
     CDS             29928..30236
                     /gene="lapA"
                     /locus_tag="JNZ73_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HAY8731731.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            30243..31412
                     /gene="lapB"
                     /locus_tag="JNZ73_001406"
     CDS             30243..31412
                     /gene="lapB"
                     /locus_tag="JNZ73_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HAY8731732.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            31605..32342
                     /gene="pyrF"
                     /locus_tag="JNZ73_001407"
     CDS             31605..32342
                     /gene="pyrF"
                     /locus_tag="JNZ73_001407"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAY8731733.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNAHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            32342..32668
                     /gene="yciH"
                     /locus_tag="JNZ73_001408"
     CDS             32342..32668
                     /gene="yciH"
                     /locus_tag="JNZ73_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HAY8731734.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(32794..33012)
                     /gene="osmB"
                     /locus_tag="JNZ73_001409"
     CDS             complement(32794..33012)
                     /gene="osmB"
                     /locus_tag="JNZ73_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HAY8731735.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVISHQVGK"
BASE COUNT         8400 a         8002 c         8420 g         8307 t
ORIGIN      
        1 ttccacagtt ttcacctgaa gcattaaata atctgaaagg tcgagtggaa acattcacac
       61 aagatttaaa ggttatcaaa ccgtcagtaa atattcagcc tcagacaatc aatatcacaa
      121 ctcagacggt acttgatgga aaggttattg atgaaagaac cacatctcat attaatcgta
      181 tgcaggaaga taccttgatt agctcagctt acccagagga ataaacaatg ttagaactac
      241 taatatttat tgccacacat ggcaatctat taatctttat ctagtatgac gattcaacta
      301 tgaatagtcg aatgctacgt gatttcttct gagcagatac caacatcaaa aattagattt
      361 cacgtagctc acgcttaaca gaataggaat aacaacaatg tttgacacaa agcaaattag
      421 atactcaggt aaagatggta tctattttca ccttagagat aatgtagaat cttttcttgc
      481 cttaacagct actgaacaga tggactatga ctcaccgatg atggtgacaa ccaggctatg
      541 caatcggggc aaactatcac tgataatgtt cagagagcac ctaagaccat ctctattagc
      601 ggtgttgtag tggttgattc gacgggttca gttctattaa ccagacaggg gcaacttgta
      661 gagaccttta tttctacggt tgaacaatgg cgagaccaga agcaaatcat cactattgtt
      721 tgtaaagatg gaatatctat cactgatagt atcattactc agtttaaagc gagtaaagag
      781 cataccattg ctaatggtct tcgtattcag atgaccttcc aaggtgtaga atttcgttca
      841 atcgtagggc aaactgatat ttcagcagct actggcaaga ccgctaccac gaatgatggt
      901 ggtgctacca gtaaaaagaa cactggcaac actacaacga gtcttggaag ccctatgctg
      961 acctgtaaag agctttttag ctattcggca agtgagttat cagatgaagc gctaaaagcc
     1021 cgtgtaacgt gctctaagag cgttagcgtt aaaaatggtg agagcacctt cacagcctca
     1081 gctaatgagc gagcttcaaa agtgcttaag tcgggtaatg ctttacggaa gtactctgtg
     1141 aatccgaata agaagggcac ttattgagac ataaaaaggg gcttcatagc ccctgaaaat
     1201 tacagttgat aagcatcaat atccagtagc tctttatcag caatttcaaa gattggtgac
     1261 ccatcattta gcggtagcgg gtctgtaggt atggtgattt cagtgtcgtt ctgtagcgtt
     1321 atagttactg aacccttacc caatgtaagc ccttttagat gtttggacaa aattaattga
     1381 cactcgacac gacccgcttt agttgtcagg tcaatatctt taagctgggg tagttcctta
     1441 tcagtgagtg aagccttacg cttcatagta tcaatcgtgg tctgtagttc tgtgcgcttc
     1501 tggttaagtt ccttaagctg aatttttagt tcatcaatat catccatctc aagaagtagg
     1561 gcggtagctt tcttgattct ggctgtgaca atttcaacct catgctgtaa cgctgcaatc
     1621 ttcctctctg attctccatc attccccacg tcaatcatcc ccagaaaatt atcaatcatc
     1681 catcggtcaa acggtttacg cttcaacggt ggaacatcac agcgaccgtc taagtggtta
     1741 ttgcacctgt agaccccagc atacttattc tttgttccgg taggatggac actagcccca
     1801 cactgacacc taaacagacc tttgaagatg ttaatcgtta gtgggttgtc acttgcaggt
     1861 gtacggcctt tacgattctt atcaagaatc tcttgagctt tattgaacgt tgcagcatct
     1921 atagcggctt cgtagtagtt cggtatagcg gggcggtcat tgcgctgtga tgctggtagt
     1981 gtacctatcg ttgtctctga ccttaagata gtacgcacag aatgaacgct aaatccatta
     2041 gcatctttac gtcgtgagaa tgaactaatg ttattagcct gtaggtgacg tgcaatgtta
     2101 tgcagacttt caccatctag cagcagcttg aaaatcaagc gtacagtttc agcacgttca
     2161 ttgagtttaa aagtctcagg gtcaatccat ccgggaacat tccttgttaa acgttgttca
     2221 ccagcaaggg caagctttgc acgttgttcc catttctgac taacacggaa agacttctgt
     2281 ttactctcct gatgcgctat gtagctttta acccgctgaa tgattgagat tcctaaatca
     2341 tcggatagag catcccttgt gtagattagc ccatcctgta gagtgtgaat ctcgatacca
     2401 tgagcaataa tgcgattcat caaggcttct gtttcaagat gttcaaggcg gcctatgcgg
     2461 tcgatagctt cgactagtaa aattgtccct tctggaaact cccctgattc aatgcgggag
     2521 agcatatccc caaattgccc tgataagtgt ttacctttgt aaccggatag accttcatca
     2581 ttgaaagatg ccactacctg agcatcgcta ttcttcctta accactgttt gaatagctca
     2641 ttttgtcgtc tggttgaatc agcaccttca gcaccttgtg tggctgatga gaatctcaag
     2701 tatgttatta cggttctcac ggtgataaac tcccatgcaa ttgattatca tttaaagatg
     2761 aacttataaa cgttatggct aatgctacag tgccgaatag ctacaataca atgccccttg
     2821 atttacctcg ccgcttcccc tggccgacgt tactttcggt ctgcattcat ggtgctgttg
     2881 tggcgggtct gctctatacc tcggtacatc aggttattga actacctgcg cctgcgcaac
     2941 caatttctgt cacgatggtt gcgcctgctg atctcgaacc gccacaagcc gttcagccgc
     3001 caccggagcc ggtggtagag ccagaaccgg aacctgagcc gatccccgaa ccgccaaaag
     3061 aagcaccggt ggtcattgaa aagccgaagc cgaaacctaa gccaaaaccg aagccggtga
     3121 aaaaggtaca ggagcagcca aaacgcgatg tcaaacccgt agagtcgcgt ccggcatcac
     3181 cgtttgaaaa tacggcaccc gcgcgcccca catcaagtac ggcaacggct gcaaccagca
     3241 agccggttac cagtgtggct tcaggaccac gcgcattaag ccgtaatcag ccgcagtatc
     3301 cggcacgagc acaggcattg cgcattgaag ggcaggttaa agttaaattt gacgtcacgc
     3361 cggatggtcg cgtggataac gtacaaatcc tctcagccaa gcctgcgaac atgtttgagc
     3421 gtgaggtgaa aaatgcgatg cgcagatggc gttatgagcc gggtaagcca ggcagtggga
     3481 ttgtggtgaa tatcctgttt aaaattaacg gcaccaccga aattcagtaa gcagaaagtc
     3541 aaaagcctcc gaccggaggc ttttgactat tactcaacag gtaaggcgcg aggttttcct
     3601 tcaggatcaa ccgcgacata cttaaataat gcttctgtcg ctttatagcg ttgcccgatt
     3661 ggttcagacg ctactttttt cacccacact tcaatattaa tgctgaccga tgtcgtccct
     3721 ttctggacac agcgtgcata gcagcacacc acatcgccga ccgcaaccgg ccgtaagaaa
     3781 gtcattcctt caacccgcac agtcactacg cgaccgtggg caatttcttt tgccagaata
     3841 gcgccgccaa tatccatttg tgacattaac caaccaccaa agatgtcacc attggcattg
     3901 gtatcggcgg gcatggctaa agtacgtaaa acaagatcgc cctgagggac gttatgtgtt
     3961 gtagacatgg taaaaccgac ttaaaaggaa aatcacaggc gcgatgctac tatgattttc
     4021 ctgaggagaa cagaggagat acagcgccag ccccgaagga ctggcagtct ggttaggatt
     4081 tatcttcctg cggcatgtgg cggtagatat agataccgct taacaatgtg aagattaagg
     4141 taagggcggt caggccaaag actttaaagt tgacccaaat attttgcggc agccagaatg
     4201 cgatgtagat gtttgccaga ccgcaaagga taaagaaaac agcccaggcc agatttagct
     4261 tcgaccatac cggttgcggc agcgtgagtt ctttacccag catccgctga attagcggct
     4321 ttttcatcac ccattggctg actaacaggg ctaccgcaaa cagagcataa atgactgtaa
     4381 ccttccattt aataaactca tcattgtgga agaacagcgt caagccaccg aagacgacca
     4441 ccagaacaaa agtgatcagg gccatcttct caaccttacg aaagcgaacc cagctataga
     4501 taagcacaat cgccgtggcg acgatcagcg ctgcagtagc cgcatagatg tcataaatct
     4561 tgtaaaacgc gaaaaagaca accagcggta aaaaatcaag aaactgcttc attttacgat
     4621 tccgtaatca aatgcttaag gttattggcg aatcaacata tacaggcgga acagatagat
     4681 gagcaatatg gcagaaatca ggttgctcaa ggtgttcgcc agtacggcac caatttccgg
     4741 ggttaatgcg gcaaaagaag aggcaaaaag cagcaacagt gtttttgcca gcaaccagct
     4801 cagtactgcg ggtgccacca gacgcatatt cgcccaagtc agccgcatac tgctacgcat
     4861 cgaggcaaaa atgcccattt tgtcctgtac cagcatcacc ggtgccaggg ccagtaaaat
     4921 ggccatgata attcccggaa cgaccaccag catgatgcca atctgtacta aaagggtagt
     4981 cagaaaaatc agaataaata acttgggcaa tatcggcgca ctggcaccaa tagctcgcag
     5041 cgcacagact ctctgacccg cagacaccag ctggataatt aatattacgc ctccggcgag
     5101 aatggcgtta ccgattaatt ctgaaaaagt ggacgccgct gaagcctgca gcaaaatttg
     5161 ttgctgttcc ggtgacatat tctgaaccag gtcgaacaac ccactactgc cgctaacggg
     5221 cacgccgtca ttgagctgcg caagctgtgc atcactgggt gagaaaacat gccctaacac
     5281 cactgtgata aacgcacata gcaacgatac caacagaatg gtcataaatt gattacggaa
     5341 gaaatttccg gtgtcacggt atacggactg cgccgtgata gacatgcact ctccttgagt
     5401 tttatgcagg tgttaattag cgggcaattg taccctggtt aagcctcggg tggcagcatc
     5461 aggtcttgta tggaaaagca tatctttgta aagcggaggt aattgctggc cttcaggcgt
     5521 gggcaaaccg cggcaagtgg cgatcgagtt aatatttgcg tagagaaaat atttaaaaat
     5581 tgatttaaat cacattaacc aggattctca atgcaacttc taaattaatc cagatcaata
     5641 aatggtgaat tatcatatgt aatgtgatct atgtaggatc atttgttact ccaatgtagg
     5701 tatattcgtc acgtttttat aaccataacg acggagcgga tatgaaaaag ttaacagtgg
     5761 cggctttggc agtaacaact cttctttctg gcagtgcctt tgcgcatgaa gcaggcgaat
     5821 tttttatgcg tgcaggttct gcaaccgtac gtccaacaga aggtgctggt agtacgttag
     5881 gaagtctggg tggattcagc gtgaccaata acactcaact gggcttgacg tttacttata
     5941 tggcgaccga caacattggt gtggaattac tggcagcgac gccgttccgc cataaaatcg
     6001 gcacccgggc gaccggcgat attgcaaccg ttcaccatct gccgccaaca ctgatggcgc
     6061 agtggtattt tggtgatgcc agcagcaaat tccgtcctta cgttggggcc ggtattaact
     6121 acaccacctt ctttgataat ggatttaacg atcatggcaa agaggcgggg ctttccgatc
     6181 tcagtctgaa agattcctgg ggagctgccg ggcaggtggg ggttgattat ctgattaacc
     6241 gtgactggtt ggttaacatg tcagtgtggt acatggatat cgataccacc gccaattata
     6301 agctaggcgg tgcacagcaa cacgatagcg tacgcctcga tccgtgggtg tttatgttct
     6361 cagcaggata tcgtttttaa ttccgcacaa aaacgacccc gtaatatacg gggtcaataa
     6421 ggacatggta taaagggtat tatttcttcg cttctacgca atcagtttca gaacgaatta
     6481 aaaatttctc agttgtttgc ggaatatgct gaatcagcca gtcggccatt tgcttttcct
     6541 catttaaaat cgcttcgatg atcggaattg agactgtatc accggcattt tttgctgctg
     6601 ctaatagtga ggtgtaacag gcgatttcaa attgctcgaa gacatatccg ctaatagagc
     6661 ctttaactat ttcatcagaa gggaatatac caccgattga ctgcccaagc gcagccattt
     6721 tactcatgga atctttaatg actgaacgtg aaatgtcatt acgatcaaga atagtttcca
     6781 gttgaacaat ctggtttttg gtttcactaa gatgttgttc aatacgagcg cgtagttcag
     6841 gataattatc tatacggctg gccatggatt caagcataga ttcggcttgc ttttccattg
     6901 cgtgggcgtc acgtaaccag tcatgataat gttcaatacg attcatgaaa atgtcctctt
     6961 tataaaatga ctaaaagtta aaattcatat ttcaggcttt attttcggct ttcttattta
     7021 cgttattaag ggccagatca gtcagtttga tgtcggtggc cttttcttct tccagggttt
     7081 ctttcagaag cttcgctgct ttacggtaac ctaattgttc agccagcgtc gctaatgtcc
     7141 cgtaactggc aatctcataa tgctcgactt tctgtgctgc ggcaatcagt gcggcatcac
     7201 gcacttcgtt tttctcggta ctttcgatga cctcattagc ttcttcaata agaccttcca
     7261 ttgccacaca tttcatgcgc ttaattttca gatttgattt tgattccaca acttgatcaa
     7321 ttcgttcaat ctgtccatga gtttcctcga ggtgcgcatg aaaagcctga cttaattttt
     7381 catttgatgt tgctcttgcg agttttgcca gtgcccgggg taattgtttt tctgcgctgt
     7441 aggtatctga aagcagttga ataaatacat cttcaatggt cttcatattc atatttttct
     7501 ccagtgaaat cactgcgagc catacggccc gcagatgcaa ttatctttat cagctgatat
     7561 taataattaa cggtgaatca ggatttgtct gatttattac caccgctttg ttgaccgcct
     7621 tttttacccg cttcagatgc gcgttgcgga tcatttttaa aattaccgcc gctatgctga
     7681 ccgcctttac ggcctgcgtc ggatgccttc tcacggtctt cggcgaaatt tcctgaacca
     7741 ccacgatgtt cggccatgtt atttctcccg ttgcgttgca ttgtttcatt aatatgagtg
     7801 ttgtgtgtcg acactcatta aaattagtcg ctaatgagaa ttagtcaaat taagcgcaac
     7861 gagaagatag agggaaaata tattttgagg aacattctgg atatattaac aattacctga
     7921 ggaataaatg acttagagaa aattattgat tttactggtg ttatgttgcg ggattaattt
     7981 gtttgattat attactgttg ggcggaaaaa tgacgtaagt tgacgttcga ccggggtaag
     8041 cgaaacggta aaaagataaa tattaaatga atttaggatt tttccggctt cattaaagaa
     8101 agttaaaatg ccgccagcgg aactggcggc tgtgggatta actgcgcgtc gccgctttca
     8161 tcggttgtac aaaaactttc agtgccgcca gcattttctc tggctcatta atatgttgct
     8221 cgatgatttt aacaatggcc gaaccagaaa tcgcgcccgc agctcctgca tcaatcgctg
     8281 cttttacctg atccggggcg gaaataccaa acccctgcaa tggaggtgca gcgttgtact
     8341 ctttcagctt cgcaaccaga tgattgaggg gtaacgcggc gcggttttct gcgccggtca
     8401 cgcccgctcg tgacagcaaa tatgtgtaac cacgaccgta agaggctatc tggcgcagta
     8461 ggtcgtcatc ggcatttggc gggcagatga agataggtgc gacattatgg cgcaacgcgg
     8521 cctggcggaa gggcgcggac tcttcaactg gcacatcggc aaccagcacc gaatcgacgc
     8581 cgactttctc gcactgggca taaaactcat caatgccttt gttaaacacc agattggcat
     8641 acatcaacag gccaatggga atggtcgggt gtttctggcg aatcagtgcc agcatttcaa
     8701 aacattgtgc cggagtcaca cctgccgcaa aggcgcgcag agtggcgttt tgaatggtcg
     8761 ggccatccgc cagtgggtcg gagaagggga tacctaactc cagcgcgtca gcaccggctt
     8821 caattagcgt atcgataatt ttcaatgact gctcaatgcc cggatcaccg agcgtgacga
     8881 aaggaatgaa tgcgccttct ttgcgctcct tcaactgggc aaacagagat tcgtagcgtt
     8941 ccatcagatt tcccctcgtg ctttcaaaat atcgtgaacg gtgaagatgt ctttatcgcc
     9001 gcgaccggaa aggttaacca ccagtagctg ctctttatcc gggttttcgc gcatcatttt
     9061 cagcgcatgg gccagggcgt gggaggattc cagcgccggg atgatccctt cgtgcaggca
     9121 cagcgttttg aaggcttcaa gggcttcatc atcggtaata gacacgtaat cagcgcgtcc
     9181 agtgctgtta agatacgcgt gttgtgggcc gacagacggg aaatccagtc cggcggagat
     9241 ggagtaagat tcttcaatct gcccgtcttc ggtttgcatc atcggcgctt tcataccgaa
     9301 ataaatgccc acgcgaccat gttttagcgg tgcgccgtgc tcgccagttt cgataccgtg
     9361 accaccaggc tccacaccaa tcaggccgac gttggtttca ttgatgaaat cagcaaacat
     9421 gccgatggca ttcgaaccgc cgccaacaca ggcgataacg gcatccggca ggcgaccttc
     9481 tctttccaga atctgcgctt tggtttcttc gccaatcatc cgctgaaact cacggacaat
     9541 ggtcggataa ggatgcgggc cagccgcggt gcccagcata tagtgcgcgg tttcataact
     9601 gccggaccag tcgcgtagcg cctcattaca ggcatctttc agcgtggcgg aaccgctatg
     9661 taccgggatc acttccgcac ccattaagcg catacgaaaa acgttaggcg actggcgttc
     9721 aacgtctttg gcacccatat aaatacggca tttcaggccg agcagggcgc tggcaagggc
     9781 cgacgccacg ccatgctgac cggccccggt ttcggcgatg atttcggttt tacccatccg
     9841 cttcgccagc aacgcctgcc ccagcacctg gttggtttta tgtgcgccgc cgtgcagcaa
     9901 atcttcacgc ttgagataca gcgtggtgtt cgtcccggct gtaatgttct ggcatttggt
     9961 cagtgcggtt ggacgcccgg catagttttt cagcaggtcg ttgaactggg cttgaaattc
    10021 aggatctttt tgcgcgctga caaaagcttc ttccagctgg cgcagagcag gcatcaggat
    10081 ttgtggcacg tacatgccgc caaactcacc aaaatagggg ttaagtaatg ttgtcattgt
    10141 tcctttcctt aatatgcgcg cagcgtctgg aaaaccgagg ccaaaagacg tgcgtctttg
    10201 atgcccggtt gcgactctac agcagaatta aaatcaagtc cggcgcagcc ggtttgtgcc
    10261 gcttccacgc agttatctgc gcctaagccc cccgccagca gaacgttgcc aagcgattga
    10321 ccatttaata gtgaccagtc aaaacgttgc ccgcttccac cctggccgtt gtctaaaaca
    10381 tatttatcaa cgtgctgaaa ctcgcgggcg ggcagggttt caccgacgct taatgctttc
    10441 cagatggcaa catgtgctgg cagagcttca cgcagcgtat cgatatacag ctgatcttca
    10501 ttaccatgca gttgcactgc cgccagcgat aacaccttag ctttgtccac cacatcggca
    10561 atatcgtgat tgcggaacac gccaacatac tgcaacggtg ccgcagccat cacttcctgc
    10621 gcctgttcaa cgttgacgca acgcggtgat gtcgcaacaa aaatcaaccc accgtaaatc
    10681 gcgcccgcgt cataagctgc tttagcatct tgcccacgcg tcaggccaca tactttattc
    10741 tcacccagca acacccggcg cacggcggcg tgcaaatcgt catgggccat caacgccgaa
    10801 ccaatcagaa aaccgttagc gaagtggctt aactcgcgca cctgagcgta agtattgatg
    10861 ccggattcgc tgattaccgt tacgttgtgc cccagtttcg gcgcaagctc gcgggtacgg
    10921 ttgagatcaa tcgacaaatc acgcagatcg cggttgttga tgccaacgac ctttgcccct
    10981 aatgcaatgg cgcgctccag ttcctcttca ttactgactt cggtcagcac ccccatctcc
    11041 agactgtgag cgacggctgc aagctggcga tattggtcgt catccagtac tgaaagcatt
    11101 aataagcagg catcggcctg gtaatagcgc gccagataga tctggtaagg gtcgataatg
    11161 aagtctttac ataaaatcgg ctgcggggcg atttggctga cgatgaggag gaaatcaaag
    11221 ctcccctgaa aatatttctc atcagtcagc accgaaattg ccgaagcgta atgtttataa
    11281 atggcggcaa tgcgtgccgg atcgaaatca tcacggatca cgccttttga cggcgacgct
    11341 tttttacact ccagaataaa cgccgtgcgt gcaccttgta gcgcatcata aaaatgtcgt
    11401 gtgctcggct gaacctcatt ctgaaaactg gccagcggtt gctgctgttt gcgggtttct
    11461 acccaaatcg ccttgtctgc gacgattttc gctaaaacgg tttgcatcat ttaccctcgt
    11521 gccgccagcg cggtaactct gtcgtaagcg gaaccactgc gcagtacctc aagaacggtt
    11581 tgcgcattgg cttgcagatc ttcatggcca tgcaggcgca ttaacatggc gacgttcgca
    11641 gcgacggctg cttcatgggc ggcgtcgcct ttaccttgta acaagcgtgt taaaatgtca
    11701 cggttttctt caggtgttcc gcctgccagt tgctcctggt ggtagggagt caggccaaaa
    11761 tcttcagcgg tcaattgata gctcttaatt ttgccgtcat gcagctcggc aacgattgtc
    11821 ggcgcgtgta atgaaacttc atccatcccg ccgctgtgca ccaccgccgc gcgttgatac
    11881 cccagcacgc gcaaggtttc ggcaatcggc agcaccagtt ccggactata aacaccaatt
    11941 aacgccagcg gcggatgcgc cgggttaatc aatggcccca gcacattgaa cagggtgcgg
    12001 gttttcagtt gctggcgaac cggcatcgcg tggcggaatc cggtgtgata tttcggcgca
    12061 aagaggaaac atacgcctaa ctcgtccagc gcctggcgcg atttattggc gttcatatca
    12121 agattaatac cgaatgccgc cagcagatcc gacgagccgg atttactgga gacgctgcgg
    12181 ttaccgtgtt tcgccacttt cagcccacag gccgcggcga caaacgcact ggcggtagaa
    12241 atattgatac tgttgctgcc gtcaccgcca gtaccgacga tatcagcaaa cagataatcc
    12301 gggcgcggga acggcgctgc gttttccagt agcgcggttg ctgccccggc gatctcgttc
    12361 gggtgctcac cacgaatttt catgctcacc agcgccgccg ccagttgttc cggcttcagc
    12421 tcgccacgca ccaccgctga aaacagctgg tggctttctt gttggctaag cgtctgcgcc
    12481 tgatacagtt tttccagaat cggttgcagc gtgttggctg gctctagttt ctgctgcgcc
    12541 caggccagcg tttgttccag caggcgagcg ccctgggtgg tgagaatgga ttccggatgg
    12601 aactggaatc cacaaacgcg atccgcatcg tgacgtactg ccatcaccat gccattaaaa
    12661 tgggcgttga tggttaaacc ggccggaatg ttactgccaa ccagcgagtg ataacgcgcc
    12721 accggcagcg ggtttgttaa tccggcaaac atcgcctgac cgtcatgttc aatgctcgac
    12781 gctttaccgt gaagaatttc gcccgcctga ccgacatagc ccccgtaagc ttcgacaatt
    12841 gcctgatgtc cgaggcaaat gccaataatg ggcagcttgc cacgcaagcg ggtgaggagt
    12901 tccggcatac aaccggcttc gctcggcaca ccggggccag gagaaagcat cagtaccgga
    12961 ttgctcatcg tcgccaggcg ttcaattaag gtctgcgccg gaatatggtt gcggtaaatc
    13021 accacgttat gaccattgct gcgcaactga tctgccaggt tgtacgtaaa agagtcgata
    13081 ttatcgagca gcagaatgtc agccatcaga aagtctcctg tgcatgatgc gcggtggcaa
    13141 tagcgcgcag tacagcgcgg gctttgttac gggtttcgtc ggcttccgac tgcggaacag
    13201 aatcaaggac tacaccagca cccgcttgca cggtggcgat accgttttcc accagcgccg
    13261 agcggatcac aatgcaggtg tcgagatcgc catgcgcggt gaaataacct accgcgccgc
    13321 cgtagctgcc gcggcgacga ccttccgcct cggcaattaa ctgcatagcg cgtactttcg
    13381 gcgcaccgct taacgtcccc atattcatac aggcgcgata agcgtgcagg gcgtcaagat
    13441 cgtgacgcag ttcgccgact acgcgagaga cgaggtgcat cacataggaa taacggtcaa
    13501 ctttggtgag atcggcgacg tagcggctgc caggggtgca aatgcgtgcc agatcattac
    13561 gggcgagatc aaccagcatc agatgttcag acagctcttt atggtcagta cgcatttcca
    13621 gttcaatacg gctgtcgaga tctctgtcca gtgaaccatc ggcgcgacga ccgcgtgggc
    13681 gtgttccggc aatcgggtag atctcaatct ggcggctggt ggcatcatac ttgagcgagc
    13741 tttccggcga cgcgccaaat agggtgaaat cattatcctg cataaaaaac atgtacgggc
    13801 tgggattact ctttttcagc acgtaatagg ccgccagcgg tgacgggcag ggcagagaga
    13861 aacggcgaga tggcaccacc tggaaaattt ctccagcgcg aatcgctttt tgcaacaaac
    13921 gcactacgcc accgaactct tcatcgctct gattacattc acaacgcata tgcggcacgg
    13981 aaaccactgg cagcggcggc gcggcttcgg tcagttgctg acgtagttcg ttcaggcgag
    14041 cagtgagacg ttgtttttct tcttcattcg gagcaaacag gctggcctga atacgggtgc
    14101 tttttttctg atggtcaatc accatcagcg tttcagcgag ataaaaacag aaatcagggc
    14161 agttattttc cgctgacagt tgcggtaaat cttcaaatcc cgccacaagg tcataagaga
    14221 acaggccgcc gaagaacatt gcttctcgtt cttccttcgg tacattcaac agattctgca
    14281 ataaacggaa agcgtcaaaa accgaaaggg agcataagcg ggcgtcttca tccagcagtg
    14341 gactgacagg ggggaagcgc agcacacggc agtttggtga ttgttcactt tccacacccg
    14401 caggcagggc gttatccagt agtgccagca gggcttcgcc attgccggaa agtgcctgaa
    14461 ttgtgacggt gtcacctaat gctgtaatgt gcagcgcatt gtctatcagc agcaggcttt
    14521 ttaaatcatc tttgctgtcg atatctgcgg attccagcag cagcgttgcc ggacgattcc
    14581 cacacaactg gtgaaaaagc gcagtcgggt tgtcgcgata agcgccttcg caggttagca
    14641 gttcgagagt cggtttttgt gtttgcattg ttattctcta attttgttca aaaaaaagcc
    14701 cgctcattag gcgggctggg tatctgattg ctttacgcat ggtgaataca ctgcccgttt
    14761 caggaagtgg ccaccaacct ttcagtacga aaattgcttt cattgtcgat accattttta
    14821 cgtgaacttg cgtactagtc aactggttcg atgattaatt gtcaacagct catttcagaa
    14881 tatttgccag acccgttatg atgtcggcgc aaaaaacatt atccagaacg ggagtgcgcc
    14941 ttgagcgaca cgaattatgc agtgatttac gacctgcaca gccataccac agcttccgat
    15001 ggctgcctga cgccagaagc attggtgcac cgtgcagtcg agatgcgcgt cggcaccctg
    15061 gcgatcaccg accatgacac cacagctgca atcgcgccag caagagagga aatttcacgt
    15121 tcgggactgg cgctgaatct tattcccggc gtggaaattt ccacggtctg ggaaaatcat
    15181 gaaattcata ttgtcgggct gaatattgat attactcatc cgctaatgtg tgagttcctc
    15241 gcgcagcaga cagaacggcg caatcagcgg gcgcagcaaa ttgccgaaag acttgaaaaa
    15301 gcgcaaatac ctggggcgct ggaaggcgca caacgactgg cgcagggcgg cgcagtgacg
    15361 cgcggtcatt ttgcgcgttt ccttgtggag tgcggcaaag ccagttcaat ggcagatgtc
    15421 tttaaaaagt atctggcgcg cgggaaaacc ggatacgttc cgccacagtg gtgtacaata
    15481 gaacaagcta ttgatgtcat tcatcattct ggcggtaagg cggtgctagc tcatcctggg
    15541 cggtacaatc ttagtgctaa atggctgaaa agattggttg cgcattttgc cgaacaccac
    15601 ggcgacgcga tggaagtcgc gcagtgccag caatcgccca atgaacgcac ccagctggcc
    15661 acacttgcgc gtcagcatca tttatgggca tcacaaggat ctgattttca tcagccatgc
    15721 ccgtggatcg aactgggtcg taaactctgg ctgcccgcag gcgttgaagg cgtctggcag
    15781 ctatgggaac agccgcagaa caccacagag agggaattat gagccagttt ttttatattc
    15841 atcctgataa cccgcagcaa cgtctgatca accaggcggt ggagatcgtg cgtaaaggcg
    15901 gggtgattgt ctatccaact gattccggct atgcgctcgg ctgtaaaatt gaagataaaa
    15961 acgccatgga gcgtatttgt cgtattcgcc agctgccgga cggtcacaac tttaccctga
    16021 tgtgtcgcga tctttctgaa ctctcgacct attcattcgt ggataacgtt gcgtttcgtt
    16081 tgatgaaaaa caacacgccg ggcaactata ccttcatcct gaaggggacg aaggaagtgc
    16141 cacgccgtct gttgcaggag aaacgcaaaa ccatcggtat gcgtgtgcct tctaacccga
    16201 ttgcgcaggc gttgcttgaa gcactgggcg aaccgatgct ttccacttcg ctaatgctgc
    16261 caggcagcga atttaccgaa tccgatcccg aagaaattaa agaccgtctg gaaaaacagg
    16321 tagatttgat tattcacggc ggctatctcg ggcagaaacc aacaacggtt attgatctta
    16381 ccgatgatac gccggtcgtg gtgcgtgaag gcgtaggtga tgtgaagcct ttcttataag
    16441 tgcgtaactc tgtatactac gcggccttaa aatggcgcta tacggcgcca ttgattctcc
    16501 tgttcgacgc ctgtgaaggc gacaccaaaa ggaagctcta tgagcgaaaa attacagaaa
    16561 gtgctggccc gcgctggcca cggctcccgc cgtgaaatcg aatctattat tgaagccggt
    16621 cgtgtgagtg ttgatggcaa aattgctaaa ctcggcgatc gcgttgaagt tacccctggg
    16681 ctgaaaattc gtatcgatgg tcatctgatt tcggtacgtg agtccgctga acaaatttgt
    16741 cgcgttctgg cctattacaa accggaaggt gagttgtgta cccgtaacga tccggaaggg
    16801 cgtccgacgg tgtttgaccg tctgccaaag ctgcgtggcg cacgctggat tgccgtaggt
    16861 cgtctggacg ttaacacctg tggtctgctg ctgttcacca ccgatggtga actggcaaac
    16921 cgtttaatgc atccaagccg tgaagttgaa cgtgaatacg ccgtgcgtgt atttggtcag
    16981 gttgacgacg cgaaactgcg tgatttgagt cgtggcgtgc aactggaaga tggtccggca
    17041 gcttttaaaa ccatcaagtt cagcggcggc gaagggatca accagtggta caacgtgact
    17101 ctgaccgaag gccgtaaccg tgaagttcgt cgtctgtggg aagcggttgg tgtgcaggtg
    17161 agccgcctga tccgtgttcg ttacggtgat atcccactgc caaaaggtct gccacgcggt
    17221 ggctggacgg agctggacct cgcccagact aactatctgc gcgaactggt ggagcttccg
    17281 ccagaaacca gctctaaagt cgctgtagaa aaagaccgtc gtcgcatgaa ggctaatcag
    17341 attcgtcgtg cggtgaaacg tcacagtcag gtgagcggcg gtcgtcgttc tggcgggcgt
    17401 aataacggtt aatcaaagta tgccggacgt catatccggc atttttacag attaataatc
    17461 aatccctatt tgcgccttaa caccggcatc aaacgcatgt ctgatggggc gcagctcact
    17521 taccgtatcc gccagttcaa gaatatcccg atgacagcct cgacccgtga taatcaccgt
    17581 ctgttgatgt ggacgttcat ttaacgcctg caccacttct tccagtggca aatagtcata
    17641 cgccaccata tacgtcagtt catcaagcaa aaccatatcc agtgaggaat cagcaagcat
    17701 ccgctttgca tgttgccaga cttcgcggca ggcggcggta tcagactcgc ggttttgtgt
    17761 atcccaggta aagcccgttg ccatcacctg aaactcaacg ccatgtggct ccagcagatt
    17821 gcgttcgcca ttaggccagg tgcctttaat aaactgcacg acgcccactt tttttccgtg
    17881 accaactgcg cgtgttgccg taccaaatgc cgcggtggtt ttgccttttc cattgccggt
    17941 aaagacgatg ataataccgc gttcatcctg ggcctgggcc acacgagcat ctactttttc
    18001 tttcactcgc tgctgacgct gttggtagcg ttcatcactc attgggaaat tcctggttta
    18061 cggcccggtt gggcgtcaaa ggtcatgccg gttttacggc ggctgtcatc gcccatcagc
    18121 cagaggtaga gcggcatgat atcagcgggt gttttaagtt tctgtggatc ttcggtcggg
    18181 aaggcgcttg cgcgcattgc ggtgcgcgta ccgcctgggt taatgcagtt gacgcgcagg
    18241 cgctgctgat attcatcggc cagtacctgc atcatccctt cggtggcaaa tttcgacgct
    18301 gcatatgcac cccagttggc tcgtccctga cgtccaacgc ttgatgaagt aaagaccagt
    18361 gaaccggcgt ccgatttgag taataaagga agcagtgcct gggtgagcat aaaggtggca
    18421 ttaacgttga cctgcatgac gtcctgccag acctgcggat tttgttcgct cattgggcaa
    18481 acatcgccga gcaatccggc attatgcaaa acaccatcca gacgcggata attaacgaca
    18541 atgcgctgtg ccagttgttg gcaattttcg gacgtgcagg tcagcaaatc gagaataaac
    18601 cactgtggct gacgcccagt ttcttcgttt atgtggctgg ctacctgacg taatttttct
    18661 tcattacggc ccaacagaat cactgtcgca ccatagcgtg catacgtcat cgcggcttca
    18721 cgaccaatcc catcgctggc tcccgtcacc aggataatgc gatcattgag taaatcttgt
    18781 tttggctggt aatgcatggc tactcctcaa cgacgttgtc tgtcgcgctg ccagtgtact
    18841 ttatgacttt ggggctttat gcctgaaaca gaagggcatt tcaatcagcc tggggcgata
    18901 acaccgcaaa atggatactt tgtcatactt tcgctgcaat aacatctctg cgagacggct
    18961 taacatgcct gttgtaaact gtgagccaaa gcgttgttta accaaggtgg ggactcgtgg
    19021 aattgttgtc tgaatatggt ttgtttttgg ctaaaatcgt taccgttgtg ctagcgattg
    19081 cggcgattgc cgccattatt gtcaatgttg ctcaacgtaa taaacgccag cgtggcgagt
    19141 tacgggtcaa caatctcagc gaacagtata aggagacgaa agaagaactg gccgcggcgc
    19201 tgatggactc acatcagcaa aaacagtggc acaaagcgca gaagaaaaag cacaagcaag
    19261 aagcgaaagc agcaaaagcg aaagccaaac tgggcgaggt ggcaactgac agtaaacctc
    19321 gcgtctgggt gctggatttt aaaggcagca tggacgccca tgaagtgaac tcgctacgtg
    19381 aagagataac ggcggtactc gcagcattca aaccgcagga tcaggttgtg ctccgtctgg
    19441 aaagccctgg tggcatggtg catggttacg ggttggcggc ttcgcagctg cagcgtctgc
    19501 gtgataaaaa cattccttta actgttacgg tagacaaagt cgctgccagc ggcggttaca
    19561 tgatggcctg tgtggcggac aaaattgttt ccgcaccgtt tgctattgtg ggttccattg
    19621 gggtggtggc gcaaatgccc aactttaacc gcttcctgaa aagcaaagat attgatatcg
    19681 aactgcacac cgccgggcag tataagcgca cgctgacctt gctgggtgaa aataccgaag
    19741 aagggcggga gaaattccgc gaagagctga acgaaacgca tcagttattt aaagattttg
    19801 tgaagcgtat gcgtccgtct ctggatattg aacaggtggc aacgggtgaa cactggtacg
    19861 gacaacaggc ggtagagaaa ggcctggttg atgaaatcaa caccagtgat gaagttattc
    19921 ttagcctgat ggaaggccgt gaagtggtta atgtacgcta tatgcagcgt aaacgactca
    19981 ttgaccgatt caccggcagc gcggcagaga gcgccgatcg attgttgcta cgctggtggc
    20041 agcggggtca aaagccattg atgtaaaaga caaacgcgag gctaagacct cgcgttttgc
    20101 tttaatcaac cagatgatat ttttctgaaa gcacatgggc caggtgtttg aacatattaa
    20161 acaccgcggt gcttttggct gttggcaatc cttgttcatc taaaaagtag tcgccggtaa
    20221 ataccagcac gccattacgc tgcgtgacgc cggtggcttc aatccctgcg agcgtatcct
    20281 catgctcacg aatgattttg ttagcctctt tcaacagcgt ttcgcggtcg atgggttgtg
    20341 tctctttgtt cattatttac tccttaaaca aggacattag tctacgccag gcatggcttg
    20401 cagacaaata taccacgctg gtggcaagag cgccttactg gcaactttgg attttgcatg
    20461 ctaataaagt tgcgtatcgg attttatcag gtacagtgtg acgctttcgt caatctggca
    20521 atagatttgc ttgacattcg accaaaattc cgtcgtgcta tagcgcctgt aggccaagac
    20581 ctgttaactc agtcacctga attttcgtga acagagtcac gacaaggggg tgatatccgc
    20641 agagagcgag tccatatcgg taactcgttg ccagtggaag gtttatcaac gtgcgacgca
    20701 ttcctggaag aatcaaatta ggtaaggtga atatgggtaa agctcttgtc atcgttgagt
    20761 ccccggcaaa agccaaaacg atcaacaagt atctgggtag tgactacgtg gtgaaatcca
    20821 gcgtcggtca catccgcgat ttgccgacca gtggctcagc tgccaaaaag agtgccgact
    20881 ctacctccac caagacggct aaaaagccta aaaaggatga acgtggcgct ctcgtcaacc
    20941 gtatgggggt tgacccgtgg cacaattggg aggcgcacta tgaagtgttg cctagtaaag
    21001 agaaggtcgt ctctgaactg aaacaactgg ctgaaaaagc cgaccacatc tatctcgcaa
    21061 ccgaccttga ccgcgaaggg gaagccattg catggcacct gcgggaagtg attggtggtg
    21121 atgatgcgcg ctatagccga gtggtgttta acgaaattac taaaaacgcg atccgccagg
    21181 catttaacaa accgggtgag ctgaatattg atcgtgttaa tgcccagcag gcgcgtcgct
    21241 ttatggaccg cgtggtgggg tatatggttt cgccgctgct gtggaaaaag atcgctcgtg
    21301 gtctgtctgc cggtcgtgtg cagtcggtgg cggtccgcct ggtggtagag cgtgagcgtg
    21361 aaattaaagc gttcgtaccg gaagagttct gggaagtcga tgccagcacg actacgccat
    21421 ctggtgaagc gttggcgttg caggtgactc atcagaatga caaaccgttc cgtccggtca
    21481 acaaagaaca aactcaggct gcggtaagtc tgctggaaaa agcgcgctac agcgtgctgg
    21541 aacgtgaaga caaaccgaca accagtaaac ctggcgctcc ttttattacc tctacgctgc
    21601 aacaagctgc cagcacccgt cttggttttg gcgtgaaaaa aaccatgatg atggcgcagc
    21661 gtttgtatga agcaggctat atcacttaca tgcgtaccga ctccactaac ctgagtcagg
    21721 acgcggtaaa tatggttcgc ggttatatca gcgataattt tggtaagaaa tatctgccgg
    21781 aaagtccgaa tcagtacgcc agcaaagaaa actcacagga agcgcacgaa gcgattcgtc
    21841 cttctgacgt caatgtgatg gcggaatcgc tgaaggatat ggaagcagat gcgcagaaac
    21901 tgtaccagct aatctggcgt cagttcgttg cctgccagat gaccccagcg aaatatgact
    21961 ccacgacgct gaccgttggt gcgggcgatt tccgcctgaa agcacgcggt cgtattttgc
    22021 gctttgatgg ctggacaaaa gtgatgcctg cactgcgtaa aggcgatgaa gatcgtatct
    22081 taccggcagt cgataaaggc gatgctctga cgctcgttga actaacacca gcccagcact
    22141 ttaccaagcc gccagcccgt ttcagtgaag catcgctggt taaagaactg gaaaaacgtg
    22201 gtatcggtcg tccgtctacc tatgcgtcga tcatttcgac cattcaggat cgtggctatg
    22261 tgcgagtaga aaatcgtcgt ttctatgcgg aaaaaatggg cgaaatcgtc accgatcgcc
    22321 ttgaagaaaa tttccgcgag ttaatgaact acgactttac cgcgcagatg gaaaacagcc
    22381 tcgaccaggt ggcaaatcac gaagcagagt ggaaagctgt actggatcac ttcttctcgg
    22441 atttcaccca gcagttagat aaagctgaaa aagatccgga agagggtggt atgcgcccga
    22501 accagatggt tctgaccagc attgactgcc cgacttgtgg tcgcaaaatg gggattcgca
    22561 cagcgagcac cggggtattc cttggctgtt ctggctatgc gctgccgccg aaagagcgtt
    22621 gcaaaaccac cattaacctg gtgccggaaa acgaagtgct gaacgtgctg gaaggcgaag
    22681 atgctgaaac caacgcgctg cgcgcaaaac gtcgttgccc gaaatgcggc acggcgatgg
    22741 acagctatct cattgatccg aaacgtaagt tgcatgtctg tggtaataac ccaacctgcg
    22801 acggttacga gatcgaagag ggcgaattcc gcattaaagg ttatgacggc ccgatcgttg
    22861 agtgtgaaaa atgtggctct gaaatgcacc tgaaaatggg gcgtttcggt aaatacatgg
    22921 cctgcaccaa cgaagagtgt aaaaacacac gtaagatttt acgtaacggc gaagtggcac
    22981 caccgaaaga agatccggtg ccattacctg agctgccgtg cgaaaaatca gatgcttatt
    23041 tcgtgctgcg tgacggtgct gccggtgtgt tcctggctgc caacactttc ccgaaatcgc
    23101 gtgaaacgcg tgcgccactg gtggaagagc tttatcgctt ccgcgaccgt ctgccggaaa
    23161 aactgcgtta tctggccgat gcgccacagc aggatccgga aggtaataag accatggttc
    23221 gctttagccg taaaaccaaa cagcaatatg tctcttcgga aaaagacgga aaggcgactg
    23281 gctggtcagc attttatgtt gatggcaaat gggttgaagg aaaaaaataa cctttaattc
    23341 tatcaggttt ttataaacaa agggtcgcga aagcggccct tttttattgc atattatttt
    23401 ttcttcacac ctatacacta aggctataaa tgatatagtg gttatagtta gcaccttttt
    23461 tattattaaa tcgtattagt cacccgccag gtgtgacgaa aaaacgatgt tctgatggcg
    23521 tctaagtgga tggtttaaca tgaaattaca acaacttcgc tatattgttg aggtggtcaa
    23581 tcataacctg aatgtctcat caacagcgga aggactttac acatcacaac ccgggatcag
    23641 taaacaagtc agaatgctgg aagacgagct aggcattcaa attttttccc gaagcggcaa
    23701 gcacctgacg caggtaacgc cagcaggaca agaaataatt cgtatcgctc gcgaagtcct
    23761 gtcgaaagtc gatgccataa aatcggttgc cggtgagcac acctggccgg ataaaggctc
    23821 gctgtatatc gccaccacgc atacccaggc acgctacgcg ttaccaaacg ttatcaaagg
    23881 ctttattgag cgttatcccc gcgtttcttt gcatatgcac cagggcccgc cgacacaaat
    23941 tgctgatgcc gtctctaaag gcaatgccga tttcgctatc gccacagaag cgctgcatct
    24001 gtatgaagat ttagtgatgt taccgtgcta ccactggaat cgggctattg tagtcactcc
    24061 ggatcacccg ctggcaggca aaaaagccat taccattgaa gaactggcgc aatatccgtt
    24121 ggtgacatat accttcggct ttaccggacg ttcagaactg gatactgcct ttaaccgcgc
    24181 agggttaacg ccgcgtatcg ttttcacggc aacggatgct gacgtcatta aaacttacgt
    24241 ccggttaggg ctgggggtag gggtcattgc cagcatggcg gtggatccgg tcgccgatcc
    24301 cgaccttgtg cgcgttgatg ctcacgatat cttcagccac agtacaacca aaattggttt
    24361 tcgccgtagt acttttttgc gcagttatat gtatgatttc attcagcgtt ttgcaccgca
    24421 tttaacgcgt gatgtcgttg atgcggctgt cgcattgcgc tccaatgaag aaattgaggt
    24481 catgtttaaa gatataaaac tgccggaaaa ataatttctt gcgttatttt cggcaccttt
    24541 tatgtagcga aggtgccgga atatattctc ttttgttact tatttctata ccgtgcacaa
    24601 tatttttatc cctcatattt atagggtaaa ttacctgtaa agtagtgtct aattacccgc
    24661 ctcgcaaatt ccagttttca aatatctatt tcaggtcaaa agattgaatt attcactgga
    24721 gacgattcgt aaattcgctg gatttttgac taaagtttct ttaggatttg tctcatcgta
    24781 tgattaatta caccaatcat tcaacgtctt gctgataagt gatggcgatt atatgaggtt
    24841 agcaatgcct tctggaaatc aggaaccccg ccgtgatcct gaattaaaac gtaaagcctg
    24901 gctgacggtt tttcttggtt ctgcactttt ctgggtggtt gtcgcactgc tgatttggaa
    24961 agtgtgggga taactatggt cggtcaggag caactggagt cgtcaccatt atgccagcat
    25021 agtgacaatg aaccagaaac gaaacgggaa tgttccgtcg ttattccaga cgactggcaa
    25081 ctaacatcgc agcagcaagc ctttatagaa ctgtttgctg aagatgatca gccgaaacaa
    25141 taattatcat cattcttatt acccattttt aatgaattaa agggctttta atacaccgca
    25201 gcaataacag cttgagttat ctcaacacaa aataataacc gttaagggtg tagcctatga
    25261 tcaacacaaa tatgaaatat tggtcctgga tgggcgcgtt ttctctgtcg atgctcttct
    25321 gggccgaact cctctggatc attactcact gatccttgac cccgctgcgg cggggttgtc
    25381 atttgctttg ccacaaggtt tctcctcttt tatcaatttg ggttgttatc aaatcgttac
    25441 gcgatgtttg tgttatcttt aatatttacc ctgaagagaa tcagggcttc gcaaccctgt
    25501 cattaaggag gagctatgtc gtcaacccta cgagaagcca gtaaggacac gttgcaggcc
    25561 aaagataaaa cttaccacta ctacagcctg ccgcttgctg ctaaatcact gggcgatatc
    25621 acccgtctac ccaagtcact caaagttttg ctcgaaaacc tgctgcgctg gcaggatggt
    25681 aactcagtta ccgaagagga tatccacgca ctggcgggat ggctgaaaaa tgcccatgct
    25741 gaccgtgaaa ttgcctaccg cccggcaagg gtgctgatgc aggactttac cggcgtacct
    25801 gccgttgttg atctggcggc aatgcgcgaa gcggttaaac gcctcggtgg cgatactgca
    25861 aaggttaacc cgctctcacc ggtcgacctg gtcattgacc actcggtgac cgtcgatcgt
    25921 tttggtgatg atgaggcatt tgaagaaaac gtacgcctgg aaatggagcg caaccacgaa
    25981 cgttatgtgt tcctgaaatg gggaaagcaa gcgttcagtc ggtttagcgt cgtgccgcca
    26041 ggcacaggca tttgccatca ggttaacctc gaatatctcg gcaaagcagt gtggagcgaa
    26101 ttgcaggacg gtgaatggat tgcttatccg gatacactcg ttggtactga ctcgcacacc
    26161 actatgatca acggccttgg cgtgctgggg tggggcgtgg gtgggatcga agcagaagcc
    26221 gccatgttag gccagccggt ttccatgctt atcccggacg tagtgggctt taaacttacc
    26281 ggaaaattac gtgaaggtat taccgccaca gacctggttc tcactgttac ccaaatgctg
    26341 cgcaaacatg gcgtggtggg gaaattcgtc gaattttatg gtgatggtct ggattcacta
    26401 ccgttggcgg atcgcgccac cattgccaat atgtcgccag aatatggtgc cacctgtggc
    26461 ttcttcccaa tcgatgcagt aaccctcgat tacatgcgtt taagcgggcg cagcgaagat
    26521 caggtcgagt tggtcgaaaa atatgccaaa gcgcagggca tgtggcgtaa cccgggcgat
    26581 gaaccgattt ttaccagtat gttagaactg gatatgaatg acgttgaagc gagcctggca
    26641 gggccaaaac gcccacagga tcgcgttgca ctgcccgatg taccaaaagc atttgccgcc
    26701 agtaacgaac tggaagtgaa tgccacgcat aaagatcgcc tgccagtcga ttatgttatg
    26761 aacggacatc agtatcagtt acctgatggc gctgtggtca ttgctgcgat aacctcgtgc
    26821 accaacacct ctaacccaag tgtgctgatg gccgcaggct tgctggcgaa aaaagccgta
    26881 actcttggcc tcaagcggca accgtgggtc aaagcgtcgc tggcaccggg ttcgaaagtc
    26941 gtttctgatt atctggcaaa agcgaaactg acaccgtatc tcgacgaact ggggtttaac
    27001 cttgtgggat acggttgtac cacctgtatt ggtaactctg ggccgctgcc cgatcctatc
    27061 gaaacggcaa tcaaaaaagg cgatttaacc gtcggtgcgg tgctgtccgg caaccgtaac
    27121 tttgaaggcc gtatccatcc gctggttaaa actaactggc tggcctcgcc gccgctggtg
    27181 gttgcctatg cgctggcggg aaatatgaat atcaacctgg cttctgagcc tatcggccat
    27241 gatcgcaaag gcgatccggt ttatctgaaa gatatctggc catcggcaca agaaattgcc
    27301 cgtgcggtag aacaagtctc cacagaaatg ttccgcaaag agtacgcaga agtttttgaa
    27361 ggcacagcag agtggaagga aattaacgtc acacgatccg atacctacgg ttggcaggag
    27421 gactcaacct atattcgctt atcgcctttc tttgatgaaa tgcaggcaac accagcacca
    27481 gtggaagata ttcacggtgc gcggatcctc gcaatgctgg gggattcagt caccactgac
    27541 catatctctc cggcgggcag tattaagccc gacagcccag cgggtcgata tctacaaggt
    27601 cggggtgttg agcgaaaaga ctttaactcc tacggttcgc ggcgtggtaa ccatgaagtg
    27661 atgatgcgcg gcaccttcgc caatattcgc atccgtaatg aaatggtgcc tggcgttgaa
    27721 ggggggatga cgcggcattt acctgacagc gacgtagtct ctatttatga tgctgcgatg
    27781 cgctataagc aggagcaaac gccgctggcg gtgattgccg ggaaagagta tggatcaggc
    27841 tcaagccgtg actgggcggc aaaaggacct cgtctgcttg gaattcgtgt ggtgattgcc
    27901 gaatcgtttg aacgaattca ccgttcgaat ttaattggca tgggcatcct gccgctggaa
    27961 tttccgcaag gcgtgacgcg taaaacgtta gggctaaccg gggaagagaa gattgatatt
    28021 ggcgatctgc aaaatctaca acctggcgcg acggttccgg tgacgcttac gcgcgcggat
    28081 ggtagccagg aagtcgtacc ctgccgttgt cgtatcgaca ccgcgacgga gttgacctac
    28141 taccagaacg acggcatttt gcattatgtc atccgtaata tgttgaagta acaagtattt
    28201 gcttgccggt tattttgctt ccggcaagca aatgaattac acaatgcaag agggttattt
    28261 gttcagcaaa tggcccattt tctcggcttt ggtatcgaga taatgttcgt tattggggtt
    28321 acgacctaca atcaatggta cgcgttcaac aatattaatc cctgcttcgg tcagaatttc
    28381 gacttttttc gggttattgg ttaacaagcg gacttcattg acgccaagga gtttgaacat
    28441 atcagcgcaa agagtgaagt cgcgctcatc agcggcgaag cctaactggt ggttagcctc
    28501 tacggtatcg taaccttgat cctgcagtgc gtaagcgcgg attttattca gcagaccaat
    28561 gttacgacct tcctgacggt gatacagcaa aataccacgg ccttcctcgg caatttgcgt
    28621 caatgccgct tcgagctgga agccacaatc gcagcgcaag ctgaacaggg cgtcaccggt
    28681 cagacattcg gaatggacgc gcgcaagtac cggggtatgc ccggaaatat cggcatagac
    28741 tagcgcgaca tgatcgtgtc cggttgccag ttcttcaaat cccaccatca ggaaatcgcc
    28801 ccatggggtt ggcagtttgg cttctgccac acgtttaagc tgcatgaaat tctccagata
    28861 atgctggttc tgtattggct tattttgcca taacgagaag gggttcacct aatcacgacg
    28921 cgtcgatcgt tcacggaatg gcacaaatct gtcaattttt cttggaactg gcattttcag
    28981 ttatgattgt gggacttatc aaaaaggaga ggccatgcgt tcgattgccc gacgtaccgc
    29041 agtgggagct gcactattgt ttgtcatgcc agtagccgta tggatttctg gctggcgttg
    29101 gcaacctgga gaacaaagtt ggcgactaaa agcggctttt tgggttactg aaactgtcac
    29161 ccagccctgg ggcgtcatta cacatttgat tttgttcggc tggtttctct ggtgtctgcg
    29221 ttttcgcatt aaggctgcct ttgtattatt ttccattctg gcggcggcaa tccttgttgg
    29281 acaaggcgtt aaatcctgga tcaaagacaa agtacaggaa ccacgacctt ttgttatctg
    29341 gttggaaaaa acacatcata ttccggttga tgagttctac actttaaagc gagcagaacg
    29401 cggaaatcta gtaaaagaac agttggctga agagaaaaat attccacaat atttgcgttc
    29461 acactggcag aaagagacgg ggtttgcctt tccttccggt cacacgatgt ttgctgccag
    29521 ttgggcactg ctggccgttg gtttgctgtg gccgcgtcgg cgaacgttaa ccattgctat
    29581 cttgttggtc tgggcaacgg gagtcatggg aagccgcctg ctactcggga tgcattggcc
    29641 acgcgatctg gtggtagcta cgttgatttc gtggacgctg gtggcggtgg caacgtggct
    29701 tgcgcaacga atttgtgggc cattaacacc acctgcggaa gaaaatcgcg aaattgcaca
    29761 acgagaacaa gaaagttaac gctggttgat tttccgaatt taggccttaa atcatcaaca
    29821 atgcgcgtgg atgccatttc gcagacggcg cgaaaatggt actttaaagg gctattgcgg
    29881 taagttgacc ataatttatt cgctctaacc acataacggg aagtaatgtg aaatatttac
    29941 tcattttctt actggtgtta gcgatcttcg tgatttcggt cacgttgggt gcgcagaacg
    30001 atcaacaggt gacgtttaat tatctgttag cgcaagggga gtaccgtatt tccacattgc
    30061 tggcggtatt gtttgctgcg gggtttgcta tcggttggtt gatttgtggc ctgttctggc
    30121 tgcgagttcg tgtttccctg gcgcgcgctg aacgtaaaat aaagcgactg gaaaaccagc
    30181 tttcacccgc gactgacgtg gctgtagtgc cgcactcgtc agcggcgaag gaataacttt
    30241 ctatgctgga gttgttgttt ctgcttttgc ctgtagccgc tgcctatggc tggtatatgg
    30301 gccgcagaag tgcgcaacaa aacaagcaag atgaagccaa ccgcttgtcg cgtgattacg
    30361 tagcgggggt taacttcctg cttagtaatc aacaggataa agcggtagac ctgtttctcg
    30421 atatgcttaa agaggataca ggcaccgttg aagcccacct tacgctcgga aacctgttcc
    30481 gttcgcgtgg cgaagttgat cgcgctattc gcatccatca gaccctaatg gaaagcgcct
    30541 cgctgaccta tgaacagcgt ctgttggcga ttcaacaact ggggcgtgat tacatggccg
    30601 ccgggttata cgaccgcgcg gaagacatgt tcaatcagct gaccgatgaa actgacttcc
    30661 gcattggcgc gctgcaacag ttgctacaaa tctaccaggc taccagtgag tggcagaaag
    30721 caattgatgt tgccgaacgc ctggtgaagc tgggtaaaga taaacagcgc gtcgaaattg
    30781 cccatttcta ctgtgagtta gccctgcagc atatggccag cgacgatctc gatcgtgcca
    30841 tgaccttgct aaaaaaaggg gcggcggcag ataaaaacag cgcccgcgta tccatcatga
    30901 tgggacgcgt gtttatggcg aaaggagaat acgccaaagc cgtcgaaagt ttgcaacgcg
    30961 tcatatccca ggacagagaa ctggtcagcg aaacgctgga aatgttgcaa acctgctatc
    31021 agcagttggg taaaactgcc gaatgggcag aattcctgca acgcgcggtg gaagagaaca
    31081 ccggtgccga tgctgaattg atgcttgctg atatcatcga agcgcgcgac ggtagtgagg
    31141 ctgcacaggt ctatattaca cgccagcttc agcgtcatcc gaccatgcgt gtattccata
    31201 agctaatgga ttaccactta aatgaagcgg aagaagggcg cgccaaagag agtctgatgg
    31261 tgctgcgtga catggttggc gagaaggtgc gtagtaagcc tcgttatagc tgccagaaat
    31321 gtggttttac cgcatacacc ctctactggc attgtccgtc ttgtcgtgcc tggtcaacta
    31381 ttaaaccgat tcgcggtctt gatggcctgt aatttttaaa aaaatccgac tttagttaca
    31441 acatactagt tattgatgtc ccattgtgct ccggcaacga cggagcagaa aaagcctgct
    31501 aggggaacaa tcgcaactgt taatttttta tttccacggg tagaatgctc gccgtttacc
    31561 tgtttcgcgc cacttccggt gcccatcatc aagaaggtct ggtcatgacg ttaactgctt
    31621 catcttcttc ccgcgctgtt acgaattctc ctgtggttgt tgcccttgat tatcataatt
    31681 gtgatgacgc gctgtccttt gtcgacaaga tcgacccacg cgattgtcgt ctgaaggtcg
    31741 gtaaagagat gtttacattg tttgggccac agtttgtgcg cgaacttcaa cagcgtggtt
    31801 ttgatatctt tcttgacctg aaattccacg atatccccaa cactgcagcg cacgctgtcg
    31861 ctgctgcagc tgacttaggc gtgtggatgg tgaatgctca tgcctccggt ggggcgcgta
    31921 tgatgaccgc agcgcgtgag gcactggttc cgtttggcaa agatgcaccg cttttgattg
    31981 ctgtgacagt gttgaccagc atggaagcca gcgacctggc cgatcttggc gtgacactgt
    32041 cacctgcaga ttatgcagaa cgtctggcag cactgacgca aaaatgtggc cttgatggtg
    32101 tggtgtgttc tgctcaggaa gctgtgcgct ttaaacaggt attcggtcag gagttcaaac
    32161 tggttacacc gggcatccgt ccgcagggga gtgaagctgg tgaccagcgc cgcattatga
    32221 cgccagaaca ggcgttggcg gctggtgttg attatatggt gattggtcgc ccggtaacgc
    32281 aatcggtaga tccagcgcag acactgaaag cgatcaacgc ctctttacag cggagtgcat
    32341 gatgagtgat tccaacagcc gtctggtcta ctcaacggaa accgggcgta ttgatgagcc
    32401 caaagcggcc ccagtacgcc ctaaaggcga tggagcggtg cgtattcagc gtcagaccag
    32461 tggacgtaaa ggtaagggcg tttgcctgat taccggtatc gatctcgatg atgccgaact
    32521 gacaaaactt gcagcggaac tgaagaaaaa atgcggctgc ggcggagcag ttaaagatgg
    32581 agttattgaa atccagggcg ataagcgtga tttattaaaa tcactacttg aagcgaaagg
    32641 gatgaaggta aaactcgcag gcggttaaca taaaaagcca cggatatatc cgtggctttc
    32701 gaatatttta ctgtgtgtat tattcagtta tttcgtactg aacagaatca agcgtattat
    32761 aattgcgagt cgataatcga catttattta cgattattta ccgacctggt gactaataac
    32821 accaccgaca gctgcaccac ctaatgtacc caacgtactg ccatcggtca gtactgcacc
    32881 gcctaatgcc cctgcacccg cgccgattgc ggtgttgcgg tcccgtttag accagttaga
    32941 acaggcactc agagacattg ccaaagtaat tgccagaaca gccgcggtca tttttttgct
    33001 cgttacaaac ataatactct ctcctgaatt tatgattcac ggaagtaagc tctctataac
    33061 tataatagct aagaataagc ctggtgaaat tattccgtga aatctgctcg cgcgcagggt
    33121 tatatcacg
//
LOCUS       DADMGC010000030        33007 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.030, whole genome shotgun sequence.
ACCESSION   DADMGC010000030 DADMGC010000000
VERSION     DADMGC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.030"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            16..4548
                     /locus_tag="JNZ73_001410"
     CDS             16..4548
                     /locus_tag="JNZ73_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY8731736.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDVWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRSSVASG
                     TDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNT
                     IALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLA
                     LGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQ
                     LYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTK
                     GKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVEANTTNITNLTDS
                     VGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKTTNDAV
                     ATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTETTSKIT
                     NVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGEDALKW
                     DKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGGNASVN
                     TDGVFTGPTYKIGETNYYNVGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSS
                     SDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTP
                     DDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIA
                     DALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALLWDKTANGGAGAYNAS
                     HDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEEN
                     GAGINYVRTNDNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALG
                     SSSVSSRVIAKGSRDTSVTENGLVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEA
                     HDAVTVRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIG
                     LNTLVLADAINGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEF
                     SVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVT
                     NIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAE
                     EENTISVGSSTNQRRITNVAASVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNIT
                     LGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSAQETKQYTDQRMVEMDNKLSKTESKL
                     SGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGST
                     NSQGEYSAALGAGIQW"
     gene            4916..6571
                     /gene="lldP"
                     /locus_tag="JNZ73_001411"
     CDS             4916..6571
                     /gene="lldP"
                     /locus_tag="JNZ73_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HAY8731737.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDITLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6571..7347
                     /gene="lldR"
                     /locus_tag="JNZ73_001412"
     CDS             6571..7347
                     /gene="lldR"
                     /locus_tag="JNZ73_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HAY8731738.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            7344..8534
                     /gene="lldD"
                     /locus_tag="JNZ73_001413"
     CDS             7344..8534
                     /gene="lldD"
                     /locus_tag="JNZ73_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HAY8731739.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            8730..9203
                     /gene="trmL"
                     /locus_tag="JNZ73_001414"
     CDS             8730..9203
                     /gene="trmL"
                     /locus_tag="JNZ73_001414"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAY8731740.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(9256..10077)
                     /gene="cysE"
                     /locus_tag="JNZ73_001415"
     CDS             complement(9256..10077)
                     /gene="cysE"
                     /locus_tag="JNZ73_001415"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAY8731741.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10157..11176)
                     /gene="gpsA"
                     /locus_tag="JNZ73_001416"
     CDS             complement(10157..11176)
                     /gene="gpsA"
                     /locus_tag="JNZ73_001416"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAY8731742.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11176..11643)
                     /gene="secB"
                     /locus_tag="JNZ73_001417"
     CDS             complement(11176..11643)
                     /gene="secB"
                     /locus_tag="JNZ73_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HAY8731743.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(11706..11957)
                     /gene="grxC"
                     /locus_tag="JNZ73_001418"
     CDS             complement(11706..11957)
                     /gene="grxC"
                     /locus_tag="JNZ73_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HAY8731744.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12099..12530)
                     /locus_tag="JNZ73_001419"
     CDS             complement(12099..12530)
                     /locus_tag="JNZ73_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAY8731745.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            12775..14319
                     /gene="gpmM"
                     /locus_tag="JNZ73_001420"
                     /gene_synonym="pgmI"
     CDS             12775..14319
                     /gene="gpmM"
                     /locus_tag="JNZ73_001420"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAY8731746.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14329..15612
                     /gene="envC"
                     /locus_tag="JNZ73_001421"
     CDS             14329..15612
                     /gene="envC"
                     /locus_tag="JNZ73_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HAY8731747.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKEHAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQVLNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15616..16575
                     /locus_tag="JNZ73_001422"
     CDS             15616..16575
                     /locus_tag="JNZ73_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HAY8731748.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQHEAAKFNASAN"
     gene            complement(16562..17596)
                     /gene="waaH"
                     /locus_tag="JNZ73_001423"
     CDS             complement(16562..17596)
                     /gene="waaH"
                     /locus_tag="JNZ73_001423"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HAY8731749.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(17835..18860)
                     /gene="tdh"
                     /locus_tag="JNZ73_001424"
     CDS             complement(17835..18860)
                     /gene="tdh"
                     /locus_tag="JNZ73_001424"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAY8731750.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(18870..20066)
                     /gene="kbl"
                     /locus_tag="JNZ73_001425"
     CDS             complement(18870..20066)
                     /gene="kbl"
                     /locus_tag="JNZ73_001425"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAY8731751.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(20341..21198)
                     /gene="yibB"
                     /locus_tag="JNZ73_001426"
     CDS             complement(20341..21198)
                     /gene="yibB"
                     /locus_tag="JNZ73_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HAY8731752.1"
                     /translation="MKSSTTIITAYFDIGRGDWTTNKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKSYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            21502..22434
                     /gene="rfaD"
                     /locus_tag="JNZ73_001427"
     CDS             21502..22434
                     /gene="rfaD"
                     /locus_tag="JNZ73_001427"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HAY8731753.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            22444..23490
                     /gene="rfaF"
                     /locus_tag="JNZ73_001428"
     CDS             22444..23490
                     /gene="rfaF"
                     /locus_tag="JNZ73_001428"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HAY8731754.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23494..24486
                     /gene="rfaC"
                     /locus_tag="JNZ73_001429"
     CDS             23494..24486
                     /gene="rfaC"
                     /locus_tag="JNZ73_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HAY8731755.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            24483..25691
                     /gene="rfaL"
                     /locus_tag="JNZ73_001430"
     CDS             24483..25691
                     /gene="rfaL"
                     /locus_tag="JNZ73_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HAY8731756.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(25727..26869)
                     /locus_tag="JNZ73_001431"
     CDS             complement(25727..26869)
                     /locus_tag="JNZ73_001431"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HAY8731757.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(26878..27891)
                     /locus_tag="JNZ73_001432"
     CDS             complement(26878..27891)
                     /locus_tag="JNZ73_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HAY8731758.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(27916..28623)
                     /gene="rfaY"
                     /locus_tag="JNZ73_001433"
     CDS             complement(27916..28623)
                     /gene="rfaY"
                     /locus_tag="JNZ73_001433"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HAY8731759.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(28649..29656)
                     /gene="waaO"
                     /locus_tag="JNZ73_001434"
                     /gene_synonym="rfaI"
     CDS             complement(28649..29656)
                     /gene="waaO"
                     /locus_tag="JNZ73_001434"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HAY8731760.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(29699..30496)
                     /gene="rfaP"
                     /locus_tag="JNZ73_001435"
     CDS             complement(29699..30496)
                     /gene="rfaP"
                     /locus_tag="JNZ73_001435"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HAY8731761.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(30489..31613)
                     /locus_tag="JNZ73_001436"
     CDS             complement(30489..31613)
                     /locus_tag="JNZ73_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAY8731762.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(31610..32668)
                     /gene="rfaQ"
                     /locus_tag="JNZ73_001437"
     CDS             complement(31610..32668)
                     /gene="rfaQ"
                     /locus_tag="JNZ73_001437"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HAY8731763.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         8733 a         8070 c         7788 g         8416 t
ORIGIN      
        1 tagcgagcat cattaatgaa caaaatattt aaagttatct ggaaccctgc gacagggaat
       61 tatactgtta ccagcgaaac ggcaaaaagc cgtggcaaga aatctgggcg cagtaagctg
      121 ttaatttctg cgctggttgc gggtggaatg ttgtcgtcgt ttggggcatt ggcgaatgcc
      181 gggaatgaca acggtcaggg tgttgattac ggtagtggat cagctggcga cgtctgggtt
      241 gctataggca aaggggcgaa agcaaatact tttatgaaca ccagtggttc cagtactgct
      301 gtgggttatg acgctatagc tgaaggccaa tatagctctg ccatcgggtc aaaaacccat
      361 gcgattggtg gtgcatcaat ggcctttggg gttagtgcaa tatcagaagg cgatagaagt
      421 atagcactgg gtgcctcttc gtattcattg ggccaatact caatggccct ggggcgtagc
      481 agtgtagcta gcggtactga cagcctcgca tttggcagac aatcacttgc cagcgcagcg
      541 aacgctattg cgataggtgc tgagaccgaa gccgctgaaa atgcaactgc tattggcaat
      601 aatgcgaagg caaaagggac taatagcatg gcaatggggt tcggaagcct tgccgataaa
      661 gtcaatacta tcgcattagg aaatggcagc caggctctgg cagataatgc aatcgccata
      721 ggccagggca acaaagctga tggcgtggat gccatcgctc tgggtaatgg tagccagtcg
      781 agaggcttaa acaccattgc cttaggcaca gccagtaatg caactggtga taagagtctt
      841 gcgcttggta gtaatagcag tgccaacggt attaactctg tcgcgctggg cgcagattcc
      901 attgcggatt tagacaatac cgtctctgtc ggcaatagtt cattaaaacg caagatcgtt
      961 aatgtgaaaa atggcgcgat caagtctgac agttacgatg ccattaatgg ttcacagctt
     1021 tatgccatta gcgactcggt agcaaaaagg cttggaggag gggctgcagt agatgttgat
     1081 gacggtactg ttacagcacc aacctacaat ttaaaaaatg gtagcaaaaa taacgtaggg
     1141 gctgcgctcg ctgtacttga tgaaaacacc ctgcaatggg accaaaccaa aggcaaatac
     1201 agcgctgctc atggtactag tagcccaact gccagcgtaa tcaccgatgt tgcggatggc
     1261 acgatttcag cctccagtaa ggatgcggtt aacggttccc aactgaaagc taccaatgac
     1321 gatgtcgaag ccaacaccac caatatcacc aacctgacgg attccgttgg tgaccttcag
     1381 gctgatgccc tgctctggaa cgaaactaaa aaggcattca gtgcagctca cggccaggat
     1441 accaccagca aaatcaccaa cgttaaagat gccgacctga cggctgacag cactgatgct
     1501 gttaacggct ctcagctgaa aaccaccaac gatgctgtgg cgacgaatac caccaatatc
     1561 gccaataaca cttccaatat tgccactaac accaccaaca tctctaacct gactgagacg
     1621 gtgactaatc ttggtgagga tgcgctgaaa tgggataagg acaatggtgt attcacggca
     1681 gctcatggca ccgagaccac cagcaaaata accaacgtta aagatggcga cctgacgact
     1741 ggcagcaccg atgccgttaa cggctctcag ctgaaaacca ccaacgatgc cgtggcgacg
     1801 aataccacca atatcgccac taacaccacc aacatctcta atctgactga gacggtgact
     1861 aatcttggtg aggatgcgct gaaatgggat aaggacaatg gtgtcttcac tgcagctcat
     1921 ggcaacaata ccgccagcaa aatcaccaat atcctggacg gcacagtcac tgcaaccagt
     1981 tccgatgcca ttaacggtag ccagctttat gacttaagca gcaatatcgc cacctacttc
     2041 ggcggcaatg cttctgtgaa tactgacggc gtgtttaccg gtccaaccta caaaatcggt
     2101 gaaacaaatt attataacgt cggcgatgct ctgctttggg atgccaccgc aggtaaattc
     2161 agtgccaaac acggtactaa tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa
     2221 atttcagact ccagttctga cgcagtaaac ggctcacaac tccacggcgt gagcagttat
     2281 gttgttgatg cgctgggggg tggtgccgaa gtcaatgcag acggcaccat cactgcgccg
     2341 acgtacacca ttgctaatgc tgattacgat aatgtcggtg atgccctgaa tgctatcgat
     2401 accactcctg acgacgctct gctctgggat gcggacgccg gtgaaaatgg tgcatttagc
     2461 gccgctcacg gaaaagataa aactgccagt gtaatcacta acgtcgctaa cggtgcaatc
     2521 tctgctgcca gcagcgacgc gattaacggc tcacaactct ataccaccaa taagtacatc
     2581 gctgatgcgc tgggtggtga cgcagaagtc aacgctgacg gcaccatcac cgcaccgact
     2641 tacaccattg cgaacgccga gtacaacaac gtcggtgacg ccctggatgc gcttgatgat
     2701 aacgccctgc tgtgggataa gactgccaat ggcggtgctg gagcctacaa tgccagccat
     2761 gacggtaaag ccagcatcat cactaatgtc gctaatggca gtattagtga ggacagtacc
     2821 gatgcagtga acggttctca gttgaatgcg acgaatatga tgattgagca gaacacccaa
     2881 attatcaatc agctcgctgg taacaccgac gcaacctata tcgaagaaaa tggcgctggt
     2941 atcaactacg ttcgtactaa cgacaacgat ttagccttta acgatgcaag cgcctctggt
     3001 gtcggcgcta cagctgtagg ttataacgct gtcgcgtctg gtgccagcag cgtagccatt
     3061 ggtcagaaca gcagcagcac cgttgatacc ggtattgcgc tgggtagcag ctccgtttcc
     3121 agccgtgtga ttgccaaagg ttctcgtgac actagcgtaa cggaaaatgg cttggtgatt
     3181 ggttatgaca ccactgacgg cgaactgcta ggtgcattgt caattggtga tgacggtaaa
     3241 taccgccaaa tcatcaacgt agctgatggt tcagaagccc atgacgccgt tacggttcgc
     3301 cagttgcaga acgctattgg agcggtcgcc actacgccaa ccaagtactt tcacgccaac
     3361 tcaacggcag aagactcact ggccgttggt gaagactcgc tggcaatggg tgcgaaaact
     3421 gtcgttaatg gtaatgcagg tattggtatc ggtttgaaca ctctggttct ggctgatgcg
     3481 atcaacggca ttgctatcgg cagtaacgca cgcgcaaacc atgcaaacag tatcgcaatg
     3541 ggtaacggtt ctcagaccac ccgtggtgca cagactggct acaccgccta caacatggac
     3601 gcaccgcaga actctgtagg tgagttttct gtcggtagtg aagacggtca acgtcagatc
     3661 acaaacgtcg cagctggttc agcggatacc gatgcggtta acgtgggtca gttgaaagtc
     3721 actgatgagc gcgtagcgca aaatacccag agcattacta acctgaacaa tcaggtcact
     3781 aatctggata ctcgcgttac taatatcgaa aacggtattg gcgacattgt caccaccggt
     3841 agcaccaagt acttcaagac caacaccgat ggcgtagatg ccaacgccca gggtaaagat
     3901 agcgttgcta ttggttctgg ttccattgct gccgctgaca acagcgtcgc actgggtacc
     3961 ggttccgttg cagaggaaga aaatacaatc tctgtaggtt cttctactaa ccaacgccgt
     4021 atcaccaacg tagctgccag tgttaatgcc accgatgcgg ttaacgtgtc gcagctgaaa
     4081 tcttctgaag caggcggagt gcgctacgac accaaagctg atggttctat cgactatagc
     4141 aatatcaccc tcggtggtgg caacggcagt acgactcgta tcagcaacgt ctccgctggc
     4201 gtcaacaaca acgacgcggt gaactacgcg cagttgaagc aaagcgcgca agaaacgaag
     4261 caatacaccg atcagcggat ggttgagatg gataacaaac tgtctaaaac tgaaagcaag
     4321 ttgagcggtg gtatcgcttc tgcaatggca atgaccggtc tgccgcaggc ttatacaccg
     4381 ggtgccagca tggcttctat tggtggcggt acttacaacg gtgaatcggc agttgcttta
     4441 ggtgtatcga tggtgagcgc caatggtcgt tgggtctaca aattacaagg tagtaccaat
     4501 agccagggtg aatactccgc cgcactcggt gccggtattc agtggtaatg atccattaag
     4561 ttagtgtgac taagggcaaa cgccaaattg cctgatgcgt tacgcgcatc aggcaagata
     4621 aggcgctccg ggtcaacaat ctaagggcaa ttctctgatg aggattgccc ttttctttac
     4681 cagacatctc cccccacaag aattggccct accaattctt cgcttatctg acctctggtt
     4741 cacaattccc caattaaaac tcacatcaat attgataata cataacattt agttaaccat
     4801 tcattgtcat tatccctaca caacacaatt ggcagtgcca cttttacaca acgtgtgaca
     4861 aggagatgag caacagactc attacacgat gtgcgtggac tctaggagac ctgcaatgaa
     4921 tctctggcaa caaaactacg atcccgccgg gaatatctgg ctttccagtc tgatagcatc
     4981 gcttcccatc ctgtttttct tctttgcgct gattaagctc aaactgaaag gatacgtcgc
     5041 cgcctcatgg acggtagcaa tcgcccttgc cgtggctttg ctgttctata aaatgccggt
     5101 cgctaacgcg ctggcctcgg tggtttatgg cttcttctat ggtttgtggc ccatcgcgtg
     5161 gatcattatt gcagcagtgt tcgtctataa gatctcggtg aaaaccgggc agtttgacat
     5221 cattcgctcg tctattcttt cgataacccc tgaccagcgt ctgcaaatgc tgatcgtcgg
     5281 tttctgtttc ggcgctttcc ttgaaggagc cgcaggcttt ggcgcaccgg tagcaattac
     5341 cgccgcattg ctggtcggcc tgggttttaa accgctgtac gccgccggac tgtgcctgat
     5401 tgttaacacc gcgccagtgg catttggcgc gatgggcatt ccaatcctgg ttgccggaca
     5461 agtaacaggc atcgacagct ttgagattgg ccagatggtg gggcgtcagc taccgtttat
     5521 gaccattatc gtgctgttct ggatcatggc gattatggac ggctggcgcg gtatcaaaga
     5581 gacgtggcct gcggtcgtgg ttgcaggcgg ctcgtttgcc atcgctcagt accttagctc
     5641 taacttcatt gggccggagc tgccggacat tatctcttcg ctggtatcac tgctctgcct
     5701 gacgctgttc ctcaaacgct ggcaaccagt tcgcgtattc cgcttcggtg acttgggggc
     5761 gtcacaggtt gatataacgc tggcccacac cggttacacc gcgggtcagg tgttacgtgc
     5821 ctggacaccg ttcctgttcc tgaccgctac cgtaacactg tggagcatcc cgccgtttaa
     5881 agccctgttc gcatcgggtg gtgcgctgta tgagtgggtg atcaacattc cggtgccgta
     5941 cctcaataaa ctggttgccc gtatgccacc agtggtcagc gaggctacag cctatgccgc
     6001 cgtgtttaag tttgactggt tctctgccac cggcaccgcc attctgtttg ctgcactgct
     6061 ctcgattgtc tggctgaaga tgaaaccgtc tgacgctatc agcaccttcg gcagcacgct
     6121 gaaagaacta gctctaccca tctactccat cggcatggtg ctggcgttcg cctttatctc
     6181 gaactattcc ggactgtcat caacactggc actggcactg gcgcacaccg gtcatgcatt
     6241 caccttcttc tcgccgttcc tcggctggct gggggtattc ctgaccgggt cggatacctc
     6301 atctaacgcc ctgttcgccg cgctgcaagc caccgcagca caacaaattg gcgtctctga
     6361 tctgttgctg gttgccgcca ataccaccgg tggcgtcacc ggtaagatga tctccccgca
     6421 atctatcgct atcgcctgtg cggcggtagg cctggtgggc aaagagtctg atttgttccg
     6481 ctttactgtc aaacacagcc tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca
     6541 ggcttatgtc ttaacgtgga tgattcctta atgattgttt tacccagacg cctgtcagac
     6601 gaggttgccg atcgtgtgcg ggcgctgatt gatgaaaaaa acctggaagc gggcatgaag
     6661 ttgcccgctg agcgccaact ggcgatgcaa ctcggcgtat cacgtaattc actacgcgag
     6721 gcgctggcaa aactggtgag cgaaggcgtg ctgctcagtc gacgcggcgg cgggacgttt
     6781 attcgctggc gtcatgacac atggtcggag caaaacatcg tccagccgct aaaaacgctg
     6841 atggccgatg atccggatta cagtttcgat attctggaag cccgctacgc cattgaagcc
     6901 agcaccgcat ggcatgcggc aatgcgcgcc acacctggcg acaaagaaaa gattcagctt
     6961 tgctttgaag caacgctaag tgaagacccg gatatcgcct cacaagcgga cgttcgtttt
     7021 catctggcga ttgccgaagc ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc
     7081 ttcgatgtcc tgcaatcctc agtgaagcat agccgtcagc ggatgtatct ggtgccaccg
     7141 gttttttcac aactgaccga acaacatcag gctgtcattg acgccatttt tgctggtgat
     7201 gccgacgggg cgcgtaaagc aatgatggcg caccttagtt ttgttcacac caccatgaaa
     7261 cgattcgatg aagatcaggc tcgccacgca cggattaccc gcctgcccgg tgagcataat
     7321 gagcattcga gggagaaaaa cgcatgatta tttccgcagc cagcgattat cgcgccgcag
     7381 cgcaacgcat tctgccgccg ttcctgttcc actatatgga tggtggtgca tattctgaat
     7441 acacgctgcg ccgcaacgtg gaagatttgt cagaagtggc actgcgccag cgtattctga
     7501 aaaacatgtc cgacttaagc ctggaaacga cgctgtttaa tgagaaattg tcgatgccgg
     7561 tagcactggc accggtgggt ttgtgtggca tgtatgcgcg tcgtggcgaa gttcaggcag
     7621 ccaaagcggc ggacgcgcat ggcattccgt ttactctctc gacggtttcc gtttgcccga
     7681 ttgaagaagt cgcgccagcc atcaagcgcc caatgtggtt ccagctttat gtactgcgcg
     7741 atcgcggctt tatgcgtaac gcgctggagc gagcaaaagc agcgggttgt tcgacgctgg
     7801 ttttcaccgt ggatatgccg acaccgggcg cacgctaccg tgatgcgcat tcaggtatga
     7861 gcggcccaaa tgcggcaatg cgccgctact tgcaggcggt gacgcatccg caatgggcgt
     7921 gggatgtggg cctgaacggt cgtccacatg atttaggtaa tatctcagct tatctcggca
     7981 aaccgaccgg actggaagat tacatcggct ggctggggaa taacttcgat ccgtccatct
     8041 catggaaaga ccttgagtgg atccgcgatt tctgggatgg cccgatggtg atcaaaggga
     8101 tcctcgatcc ggaagatgcg cgcgatgcag tacgttttgg tgctgatgga attgtggttt
     8161 ctaaccacgg tggccgccag ctggacggtg tactctcttc cgctcgtgcg ttgcccgcta
     8221 ttgcagatgc ggtgaaaggt gatatcgcca ttctggcgga tagcggaatt cgtaacgggc
     8281 ttgatgtcgt gcgtatgatt gcgctcggtg ccgacaccgt actgctgggt cgtgctttcc
     8341 tgtatgcgct ggcaacagcg ggccaggcgg gtgtagctaa cctgctaaat ctgatcgaaa
     8401 aagagatgaa agtggcgatg acgctgactg gcgcgaaatc gatcagtgaa attacgcaag
     8461 attcgctggt gcaggggctg ggtaaagagt tgcctacggc actggctccg atggcgaaag
     8521 ggaatgcggc atagtcgttt gcgccccctc accctaaccc tctccctcag ggagagggga
     8581 ccgttcggcg ctgtatgtac tccctcactc tgaaacgaca ccgcactctt tttttctccc
     8641 tcgcccctcc ggggagggcc ggggtgaggg gaaaaggccg cacggttcac caattttctg
     8701 ctatcctgcc cccgcactaa gggggcagta tgctaaacat cgtactttac gaaccagaaa
     8761 ttccgccaaa tactggcaac atcatccgtc tttgcgctaa taccggcttt cgtctacata
     8821 tcatcgaacc gatgggattt gcctgggacg acaagcgcct gcgccgcgcg gggctggact
     8881 atcacgagtt taccgccgtt acgcgtcatc atgactatcg cgcgttcctc gaagcagaaa
     8941 atccccagcg cctgtttgcc ctcaccacga aaggtacgcc tgctcacagc gccgtaagct
     9001 atcaggatgg cgattatctg atgttcggcc cggaaacacg cggcctgcca gcgagcattc
     9061 ttgatgccct gcccgctgaa caaaaaattc gcattccgat ggtgccggac agccgcagca
     9121 tgaatctgtc caatgcggtg tcggtagtgg tgtatgaagc ctggcggcag ctgggatatc
     9181 cgggagcgtt gttgagagat tagttactgt atgccggata gatgattaca tcgcatccgg
     9241 cacgatcaca ggacattaga tcccatcccc atactcaaat gtatggttaa taccgttgaa
     9301 atgctggtcc atatccattg atggcttatc gctgtctggt ttaccgacaa tacgagccgg
     9361 aacgccagcg gcggttgtat gcggcggcac cggttgcagc accacggaac ctgcgccaat
     9421 cttcgcgccg cgaccaactt caatattgcc gaggattttc gcgcccgcgc caatcatcac
     9481 accttcacga attttcgggt gacggtcacc accagattta cccgtaccgc caagcgtcac
     9541 agattgcaga atcgatacgt cgttttcaat taccgccgtt tcacccacaa cgatgcccgt
     9601 ggcgtggtcg agcataatgc cacggccaat ttttgctgcc gggtgaatat cgacctggaa
     9661 cgtcacagaa acctggtttt gcagaaagat tgccaatgcg cgacgcccct gattccacaa
     9721 ccagtgaccg atgcgatagg cctgcaaggc atgaaaaccc ttcaggtata acaacggggt
     9781 tgagtattta tcgactgccg ggtcgcgggt acgcaccgcc tgaatatcac aggccgcaga
     9841 ggcgatcatt tccgggtcag cggcgtaggc ttcttcaacc acttcacgga tagcaatagc
     9901 aggcataatt ggcgatgaca gcttgttcgc cagcatgtag ctcagtgcac tgccaaggtt
     9961 ttcgtgcttg agtagcgtcg cgtggtaaaa actggccagc attggctcac agtccgccag
    10021 cgttctggct tcggctttaa tattgttcca gacaatttcc agttcttcac acgacattgc
    10081 ttactccaca cgatgagata atgaccggcc cgttctgcgc gggccgggtc atagcggtaa
    10141 caaaggttcc ctggggttag tggctgctgc gctcgtcctt gcgtgcacga cctaataatg
    10201 tcaatgctgc ctcgcgcgcg ttttttccgc aatataatac ttgataaatt tcctcggtta
    10261 ttggcatttc aacgccgaag cgatgcgcca gttcgcggac ttctttcgta ttgcggtagc
    10321 cttccaccac ctgaccaatc ttctcctgcg cgctttgtac atccatgccc tgaccgagca
    10381 tcatgccaaa acggcggtta cgcgactggt tgtcggtaca ggtaagcacc agatcgccca
    10441 gccccgccat gcccataaag gtggcaggat cggcacccag cgccgcacca agacgcgaca
    10501 tttcagccag cccacgggtg atcagcgccg tacgcgcatt cgcaccaaaa ccgataccgt
    10561 cagacatccc cgcgccaatg gcaatgacgt ttttcaccgc gccgccaagc tgcacgccaa
    10621 tgaaatccgg gttgctgtaa acgcggaaac ttttgccgca gtgcagcagc tgctggagat
    10681 catcggcaaa ggtctgatcg gtcgaggcca gcgaaatagc tgtcggtaaa cctgccgcca
    10741 gttctttcgc aaacgttggg ccagagataa ccgccagcgg aatttgatcg cctaaggcct
    10801 cacgcgccac gtcctgtaac agacgtccgg tttccgcttc cagccctttg gtcgcccaca
    10861 ccagacgcgc atcaggacgc atcagcggtt taatctggcg cagcacttca ccaaagacat
    10921 ggctgggtac gacgacgaga atattacgac tggctgccag cgcagtggcg agatcgcttt
    10981 caagatggag cgtatcggga aaaggcacat cggggagaaa cgcggcgtta cagcggtcgc
    11041 gttcaagcgt tgcgatatgt tcagggtcat ggccccagag gacaacctcg tggccatttc
    11101 ttgccagggt aatggcaaga gcggtgccgt acgagccggc accgatcaca gtcattgaag
    11161 cattacgttg gttcatcagg catcctgatg ttcttcagta ccttcgccag cctgctgctg
    11221 caaatagttc atgaacagcg catcgaagtt aaccggcgca aggttcagtt gcgggaatgt
    11281 accgcgggat accatgctgg tgatgcactc acgagcatac gggaacagaa tgttcgggca
    11341 gtatgcgccc aggcaatgtg ccatctgggt gccttcgata cccgcgatgg agaaaatacc
    11401 gccctgctga acttcacaca ggaacgcggt ttcttcgccc aaggaggccg ttacggtaac
    11461 acgcagtacc acttcgtata cgtcatctgc cagttgggaa gatgccgtat ccagatcaag
    11521 tttaacttct ggttgccaat ctttctggaa aacgtgcggc gcgttcggcg cttcgaaaga
    11581 gatatccttg gtataaatac gttggatctg gaaagtcatt tcagtgttgt tttgttctga
    11641 catgtgtaga aaacccttaa gtgttgtcct taaatactgc gcaatgtaat gccaacgttc
    11701 acacgttatt tcagcagggg atccagtcca ccacgtgcat ccaatgcata caagtcatca
    11761 cagccgccaa tgtgctgtgc gtcaataaaa atctggggaa ccgtggtgcg accgctgcgt
    11821 ttgatcatct cttcacgctt ggcggcattg ccatcgatcg gcaactcctg gaaactcacg
    11881 cccttgctgc tcagcagtgc ttttgcacga tggcaatacg ggcaggtttc tttggtatag
    11941 atttcaacat tggccataat ttagctcctg tttttttacc ctgaaatttc atgttgcagg
    12001 gcggctgcaa aatggagtgt ccccagaagc ttataacaat aagtcgctgg gggcctaatt
    12061 gcagctaacg gcctgcatca tgaaagacga caggtaaatt atttgccgcg caccagaggc
    12121 aagttttcgc cagcccagcc agcaacgccc tctttcagca cgaatacttg cgcaaaacca
    12181 gctttcgtca gcgcgtttgc aggctcctgg cactgcatgc cagaaccgtc taccacgata
    12241 accgatttgt ctttgtgctt ctcaagctca ccaacattgt tggctttgat ttcgctcggc
    12301 aacagattga tagaacctgc gatatggcct ttacggaagt catcacgctg acgtaaatcc
    12361 acaaccacag cgtcttcttt gttgatcaga cgcgtagctt caccacgagt aatcaccttc
    12421 actttcgagg tcaggctctt aaacgtagtc acaagaaccg ccaccagtaa cgcgatccag
    12481 gcgatactca gtatgggatg acggccaaca aattgcataa tttcttgcat ggggggtaac
    12541 aactcccaac gtagtgatta aaaaaaccag gaaaggagta tacctgcgcg gtgcggcaaa
    12601 tacagccagc gcgttaactg gaatgcaatt ttgcggggca cgacgaaaaa aaagcgcaaa
    12661 tctggcatcg gcttgccgcg agcggtcgta tattttgatc ttcagaggct attttatcga
    12721 ttcagctgta gtaaaattac gcaaattttg actcttgagt atgaggttgt cgcaatgtcg
    12781 gtttctaaaa aacctatggt actggtgatt ctggatggct atggctatcg tgaagaacag
    12841 caggataacg ccatttttag tgctaaaacc ccggtaatgg atgcactgtg ggccaatcgt
    12901 ccgcataccc tgatcgacgc ttccggtctg gaagtcggtc tgcctgaccg tcagatgggt
    12961 aactccgaag taggccacgt taacctgggt gccggccgca tcgtgtatca ggacctgact
    13021 cgtctggacg ttgaaatcaa agatcgcgct ttctttgcta atccagtgct ggctggcgcg
    13081 gtacataaag cgaaaaacgc aggtaaagca gtacacatta tgggtctgct ctccgcaggc
    13141 ggcgtacaca gccacgaaga tcacatcatg gcgatggtag aactggcagc tgaacgcggc
    13201 gcagaaaaaa tctacctgca cgcattcctt gacggtcgcg acaccccgcc gcgcagtgct
    13261 gaatcctcgc tgaaaaaatt tgaagagaaa tttgctgcgc tgggtaaagg ccgcgtagcg
    13321 tccatcattg gtcgttacta cgcgatggac cgcgataacc gttgggatcg cgttgaaaaa
    13381 gcctatgacc tgctgactct ggcgcagggt gagttccagg ccgatactgc cgttgctggt
    13441 ttgcaggcag cttatgctcg cgacgaaaac gacgagttcg tgaaagcgac cgttattcgt
    13501 gcagaaggtc agccagatgc ggcaatggaa gacggcgatg cgctgatttt catgaacttc
    13561 cgtgctgacc gcgcgcgtga aatcactcgt gcattcgtga acgctgattt cgatggcttc
    13621 gcgcgtaaga aagtggttaa cgtcgatttc gtgatgctga ccgaatacgc cgctgacatc
    13681 aaaactgcgg ttgcttaccc acccgcttcc ctggttaaca ccttcggcga gtggatggcg
    13741 aaaaacgaca aaactcagtt gcgtatttcc gaaaccgaaa aatatgccca cgttactttc
    13801 ttcttcaacg gtggcgtaga agagtcgttc aaaggcgaag atcgcattct gatcaactca
    13861 ccgaaagtgg ctacctacga tctgcaaccg gaaatgagct ccgcagagct gaccgaaaaa
    13921 ctggttgcgg ccatcaagag cggcaaatac gacaccatta tctgtaacta tccgaacggc
    13981 gacatggtag gtcacaccgg ggtgatggaa gcggcggtta aagcggttga agcactggat
    14041 cactgcgtgg aagaagtcgc gaaagcggtt gagtccgtgg gtggacaact gctgatcacc
    14101 gctgaccacg gtaacgctga acagatgcgc gatccagcaa cgggtcaggc acacacggca
    14161 cacaccaacc tgccagttcc gctgatttac gttggtgata agaacgtgaa agcggttgca
    14221 ggcggcaaac tttctgacat cgcgccgacc atgttgtcgc tgatgggtat ggaaatcccg
    14281 caagagatga ctggtaagcc gctgttcatc gtggaataat ccctccccat gaggggaaag
    14341 gcgattaata ccatgacacg ggccgtgaaa ccgcgcaggt ttgcaatcag gcccatcatc
    14401 tacgccagcg ttcttagcgc tggcgtattg ttgtgcgcct tttccgccca cgcggatgag
    14461 cgtgaccaac tcaaatctat tcaggccgat atcgccgcaa aagagcacgc ggtacgccaa
    14521 aagcaacaac aacgcgcaag cctgctcgca caattgaaaa agcaggaaga agcgatctct
    14581 gaagccaccc gtaagctgcg cgaaacgcaa aacacgctca atcaactcaa taaacagatt
    14641 gatgagatga acgcgtcgat tgccaaactg gagcagcaaa aagccgccca ggagcgcagc
    14701 ctcgccgcac aactggatgc cgcattccgt cagggcgagc ataccggtat tcagctaatt
    14761 ctcagcggtg aagaaagcca gcgtggacag cgtttacagg cttatttcgg ctatctcaac
    14821 caggcgcgac aagaaaccat tgcccagttg aagcaaacgc gtgaagaagt caccatgcag
    14881 cgtgctgaac tggaagagaa acaaagcgag caacaaacgc tgttatatga acagcgcgcc
    14941 caacaggcga aactgactca ggttctgaac gagcgtaaaa agacgctggc ggggctggaa
    15001 tcttccatcc agcaaggtca gcagcagcta agtgaactac gtgccaacga atcccgtctg
    15061 cgtaacagca ttgcccgtgc ggaagccgcg gcgaaagcgc gtgctgaacg agaagcgcgt
    15121 gaggcccagg cggttcgcga gcgccagaaa gaagcgacgc gcaaaggcac cacctacaaa
    15181 ccgaccgaaa gcgaaaaatc gctgatgtcc cgaactggtg gcctgggtgc gccgcgtggt
    15241 caggcattct ggccggttcg cgggccgacg ctgcatcgct atggcgagca gctacagggt
    15301 gaattacgct ggaaagggat ggttatcggt gcctctgaag gtactgaagt taaagcaatt
    15361 gccgacggtc gagtgattct ggctgactgg ctgcaaggtt acggtctggt ggtggtggtt
    15421 gagcacggta aaggcgacat gagtctttac ggctataatc agagcgcact ggtgagcgtt
    15481 ggttcgcagg ttcgcgcggg ccagccaatt gcactggtgg gcagcagtgg cggtcagggt
    15541 cggccttcac tctatttcga aattcgccgc cagggtcagg cggtcaatcc acagccgtgg
    15601 ttgggaagat aagttttgtt tccatttcgt cgtaacgttc ttgcatttgc cgctctgttg
    15661 gcgctctcct cccccgtact tgctggcaaa cttgccatcg tcattgatga ttttgggtat
    15721 cgcccgcaca acgaaaacca ggtgctggcg atgccttccg ctatctccgt cgctgtatta
    15781 cccgattcac cgcacgccag agagatggcg accaaagcgc ataacagcgg tcacgaagtg
    15841 ttgattcatc tcccgatggc accgttgagt aaacagccgc tggagaaaaa tacgctacgc
    15901 ccggagatga gcagcgacga aattgagcgc attattcgta gtgcggtcaa taacgtgccc
    15961 tatgccgtgg ggatcaacaa ccacatgggc agcaagatga cctctaacct gtttggtatg
    16021 cagaaagtga tgcaggcgct ggagcgttac aatctttact tcctcgacag cgtaaccatc
    16081 ggtaataccc aggcgatgcg cgccgcgcaa ggtactggcg tgaaggtgat caaacggaag
    16141 gtgttcctcg acgattcgca gaacgaagcg gacatccgtg tgcaatttaa tcgcgcaatt
    16201 gatctggcgc gtcgcaacgg ttcaaccatt gccattgggc atcctcaccc ttcaacggta
    16261 cgcgtgctac aacagatggt ttataacctg ccgccagaca ttacgctggt gaaagccagc
    16321 agcttgctga atgaaccgca ggttgatact tctacaccgc cgaaaaacgc tgtgcctgat
    16381 gcaccgcgta atccgttccg tggcgtgaag ctgtgcaaac cgaagaaacc gatagaacct
    16441 gtttatgcta atcgcttctt tgaagtatta agcgaaagca tcagccagag cacgctgatc
    16501 gtttacttcc agcatcagtg gcaaggctgg ggcaaacagc acgaagcggc gaagtttaac
    16561 gctagcgcaa attaaaggcg ctacgcgtaa tgcgatggtg cgaccgcgtt ttgtcgcgcc
    16621 attgccataa gcggaaagac cacagtaacg cctggtaacc gaccttcacg ctgcgcacat
    16681 tggtaatcag gcgcttatac ataccggaag taaatatctc ggcaatcatc cgttggcggg
    16741 taagaatatc cggctctttg cgcaccgcat ggcaaacacg caatgcttcg taagttattt
    16801 gctgatgaaa ttccggataa atcgtaattt tgtcggcata atttcgattt aatttctcca
    16861 gcaggcgggt aatcttaata tagtgacgtt gataattaag gtttttattc ccttgtctat
    16921 gcaaccgact cactgacgta ttatgcagat aatatttata taatgattgc tcggtatatc
    16981 gcgcacgcag cgcgttaaac atgaattctg ttgtccagac aatatcctga tgatgtaatc
    17041 cggcaataaa tttaatgtta tttttaacaa taacatcacg gcgataaacc cccatccaga
    17101 caacgtgagt ccaacggcgc gaagaaagcc ccatccgcag ccagtccggg ccggttaata
    17161 caccggttga gcgaaggcga tcgctgggga tggattgcca ggtttctccc gtttcacgaa
    17221 aagaccagtc agcgttgcac tgcgccacgt cgaggtcgtc ctctaacgcc atggtcatca
    17281 gcgtttcgta catggtggga tagacctcat cgtcagcatc gacaaaagcg acatatttgc
    17341 ccgtcgccac ctcgatccca cgattacgcg ctaccgatgc gccagcattc gcctgatgca
    17401 acaaacgaac gtgcggatag ttttctgcgt aatgctttgc tatttcaaca gaattatccg
    17461 ttgaaccatc gttaataata atgatttcca gagcagtcca ggtttgcgta attaaagatt
    17521 ccatacaagt gcggaaatca tcgcccgcat tatataacgg aataataaca ctaagtttat
    17581 tggtgctgtt catcataaat taccgacgac ctctgaagtt atcctgacgt tttacaggct
    17641 ttgtattaag aaactattaa gcctgtgaga tattattttt acaggtgaga gagtcacttc
    17701 agggtagaga gataatgaga acagctatat aaaagatcag cgcaattacg aactctttat
    17761 tacactgaca ggattttaca taattaacag attatcctca gataaaaggg ctggaatacc
    17821 agcccttgtt cgtgttaatc ccagctcaga ataactttcc cggactggcc cgaacgcata
    17881 gcgtcaaagc ccttctggaa atcatcgata gagaaacgat gggtaatgat cggcgagaga
    17941 tcgaggccag actgaatcag cgccgccatc ttgtaccaag tttcaaacat ctcacgaccg
    18001 taaatgcctt taatgaacaa gcctttaaag atcactttgg tccagtcgat agacatatca
    18061 gacggtggaa tacccagcat cgcaatacga ccgccgtggt tcatggtatc gagcatggta
    18121 cgaaacgctg gcggcgcgcc ggacatttcc agaccgacat caaagccttc ggtcatgccc
    18181 agttcagcca tcacatcatt aaggttttct ttcgcgacgt taaccgcacg ggtgataccc
    18241 attttgcgcg ccagctcaag gcggtattcg ttaacatcgg tgatcaccac attgcgtgca
    18301 ccaacgtgtt tcgccaccgc cgcagccata atacctatcg gacctgcacc agaaaccagc
    18361 acatcttcgc ccaccagatc gaacgacagc gcggtatgca cggcgttacc gaagggatca
    18421 aaaatggaag ccaggtcgtc ggagatattg tcggggattt tgaaagcgtt gaacgccgga
    18481 atcaccagat attcggcgaa gcagcccgga cggttaacac cgacaccaat ggtgttgcgg
    18541 cacagatggg tacgaccgcc acgacagttg cgacagtgac cgcaggtgat atggccttcg
    18601 ccagaaacgc gatcgccgat tttgaagcct ttcacttcct gaccaatacc taccacttca
    18661 ccgacatatt catggcccac gaccatcggc accggaatgg ttttttgcga ccactcatcc
    18721 cagttataga tgtgaacgtc agtcccgcag atggctgttt tacggatttt aatcagcaga
    18781 tcgttatgcc cgagttccgg tacagggacg tcggtcatcc agatgccctc ttccgctttc
    18841 agtttggata acgctttcat ctcacatcct caggcgataa cgcccagttg tttaccaata
    18901 cgcgtgaatg cttctactgc acgcgtaatt tgctcagggg tatgcgccgc agacatctgg
    18961 gtacgaatac gcgcctgacc tttcggaacg accggataga agaaaccggt gacgtagatg
    19021 ccctcttttt gcagctctcg ggcaaatttc tgcgccacta ccgcatcacc aagcatgacc
    19081 ggaataatgg cgtgatcggc tcccgccagg gtaaagcccg ccgccgacat ttgctcacgg
    19141 aactgacgcg cgttcgccca cagacggtca cgcaactcgc tacccgcttc aaccatctcc
    19201 agcactttga tggaagcggc gacaatcgct ggtgccagcg agttggagaa caggtacgga
    19261 cgagaacgct ggcgcagcca ctcaaccact tctttgcgcg ccgcggtata accaccagaa
    19321 gccccgccca gcgctttacc gagcgtgccg gtgatgatgt cgacacgccc catcacatcg
    19381 cagtattcat gggaaccacg accattttca ccgacaaaac cgaccgcgtg ggagtcgtct
    19441 accatcacca gggcatcgta tttatctgcc agatcgcaaa cgcccttcag attggcaatc
    19501 acgccgtcca tcgagaacac accatcggtg gcaatcagca catgacgcgc accggcttca
    19561 cgcgcttctt tcagacgagc ttcaagctcc tgcatatcgt tgttggcata gcgatagcgt
    19621 ttagctttac acagacgcac accatcaata atagacgcgt ggttcagtgc gtcggagata
    19681 atggcgtctt ccgcgcccag cagcgtttca aacaggccac cgttagcatc aaagcaggaa
    19741 gagtagagaa tcgcatcttc catccccagg aatgccgcca gtttttgttc aagctcttta
    19801 tggctgtcct gagtgccgca aataaaacgc accgaagcca tgccgaaacc gtgagaatcc
    19861 attcccgcct ttgctgccgc aatcagatcc ggatgattcg ccagcccgag atagttgttg
    19921 gcacaaaagt taatgacgtg gcttccatca gccacggtga tatctgcttg ctgcgcagac
    19981 gtaataatgc gctcttcttt aaacaaccct tccgcccgtg cggtttccag atcgttggtt
    20041 aactgctgat aaaattctcc acgcattgcg attctccaga cagggcaaat tccagcacat
    20101 attacccaaa cttataggtc gcgacgagat gatgcgttaa cacttctgca aaattcagga
    20161 taaataacgt gtatcttgat gtctctcaac acctacagat agtagaaaaa ctgtacagtt
    20221 ttggctgagc ataatgatga gtgattttac tgcattgata taaaaagaaa aaatgatgcc
    20281 aaagcatcat ttttcctatc tcagttggca tcaaaacgct acccactaat ggagggaaac
    20341 ctattttctt gaaaggaaaa ttctcagcgc ctgcattttt gcccctaaag tgttactcct
    20401 gaagcagcga aacaaatcga accattttcc tctccccaga tagtttaggt tgaagagatc
    20461 ggaacgtttg taatagcaca tgacaaaaat gccctggtca tcgtccacaa tgttattgtt
    20521 tagtgttatt ttttgacttt ccagaactaa tttatagaat tctttccatt tatgctgtga
    20581 accaacaatt gcgcccccaa taatatatac atgattgcca atcatgaaat caaaagcctg
    20641 ttgttgagat gtaacagtaa gccctttttt tatagtgaaa agatgcatct tattttcatc
    20701 aaatgggaaa tcccaaattt tcaatcctcg agttacattt ggcttacggc aatagccaaa
    20761 atctatccac gcaacaagag gcgttttcac taaacccatg ttgatagctt tatttacaaa
    20821 ataggactta aggttacata ccaaaacata ctctggtgac cagtactctg ggttttttaa
    20881 ttgtcgaggc tcaagtctat ttgtaaacga ctcatctttt tgaatttttt cgatacgact
    20941 tctgatatat ctaaatttct tttttatatc gataacgata accgttgttg gttttccgtt
    21001 gcgaatcgct tcaatccttg gtttcaaatc gggagaagtg aaaataatca tctcattttc
    21061 aagtgcggcc agacgctcaa agtaactaaa ataaacatca actgaacgag ctagtttttc
    21121 acggaaccct ttattagtag tccaatcccc tctaccaatg tcaaaatatg cagtgataat
    21181 tgtggtggat gatttcatta ctaataagac cattaatata aaaaaagaat tatatcataa
    21241 atattaacag aatgtatact gtatattatc cattcaaatg attccgttat ttatcataca
    21301 tcggaatatt gatactaaag cactattcac atgcaaaacc aacatccgcc atgaaggact
    21361 agctaaaacc caaactagtt tgttgcaatt agcatccttg cacctctatg taaagggctg
    21421 aagggattcg gatgtgatgg tatgattaca gacattcgtg tctgagattg tctctgactc
    21481 cataattcga aggttacagt tatgatcatc gttaccggcg gcgcgggctt tatcggcagc
    21541 aacatcgtta aggccctgaa tgataaaggc atcaccgata ttctggtggt ggacaacctg
    21601 aaagacggca ccaagtttgt gaacctggtg gatctggata tcgcggacta tatggataag
    21661 gaagacttcc tgatccagat tatggctggc gaagagttcg gtgatgtcga agcgattttc
    21721 cacgaaggcg catgctcttc caccaccgag tgggatggca agtatatgat ggataacaac
    21781 tatcaatact ccaaagagct gctgcactac tgtctggagc gcgaaatccc gttcctgtat
    21841 gcctcttccg cagccaccta cggcggacgc acctccgact ttattgaatc ccgcgagtac
    21901 gaaaaaccgt tgaatgtcta cggttactca aaattcctgt ttgatgaata tgttcgtcaa
    21961 atcctgccag aagcgaactc gcagattgtt ggcttccgct atttcaacgt ttatggaccg
    22021 cgtgaaggcc ataaaggcag catggcgagc gtcgctttcc atctcaacac ccagcttaat
    22081 aacggtgaat caccgaagct gttcgaaggt agcgagaact tcaaacgtga cttcgtctat
    22141 gtgggcgacg tggcagatgt aaacctgtgg ttcctggaaa atggcgtttc cggtatcttc
    22201 aacctcggca ccggtcgtgc agaatccttc caggcagtag cagatgctac gctggcttat
    22261 cacaagaaag gccagatcga atacattccg ttcccggata agctgaaagg ccgctaccag
    22321 gcgttcactc aggcagatct gacaaatctg cgcgcggcgg gttacgacaa accgttcaaa
    22381 accgttgctg aaggtgtaac ggaatacatg gcctggctga atcgcgacgc ataagagctc
    22441 tgcatgaaaa tactggtgat cggcccgtct tgggttggcg acatgatgat gtcgcaaagt
    22501 ctctatcgca cgctccaggc gcgctatccc caggcgataa tcgatgtgat ggcaccggca
    22561 tggtgccgtc cattattatc gcggatgccg gaagttaacg aagctatccc tatgcctctc
    22621 ggtcacggag cgctggaaat cggcgaacgc cgcaaactgg gtcatagcct gcgtgaaaag
    22681 cgctacgacc gcgcctacgt cttaccaaac tccttcaaat ctgcattagt gcctttcttc
    22741 gcgggtattc ctcatcgcac cggctggcgc ggcgagatgc gctacggttt actcaacgat
    22801 gtacgcgtgc tcgataaaga agcctggccg ctaatggtgg aacgctatgt cgcgctggcc
    22861 tatgacaaag gcattatgcg taccgcacaa gatctgccgc agccattgtt atggccgcag
    22921 ttgcaggtga gcgaaggtga aaaatcatat acctgtaatc aattttcgct ttcatcagaa
    22981 cgtccgatga ttggcttttg cccgggtgcg gagtttggtc cggcaaaacg ctggccacac
    23041 taccactatg cggaactggc aaagcagctg attgatgaag gttatcaggt ggttctgttt
    23101 ggctctgcga aagatcatga agcgggcaat gagattcttg ccgctttgaa taccgagcag
    23161 caggcatggt gtcggaacct ggcgggggaa acacagcttg atcaagcggt tatcctgatt
    23221 gcagcctgta aagccattgt cactaacgat tctggcctga tgcacgttgc ggcggcgctc
    23281 aatcgtccgc tggttgccct gtatggtcca agtagcccgg acttcacacc gccgctatcc
    23341 cataaagcgc gcgtgatccg tctgattacc ggctatcaca aagtgcgtaa aggtgacgct
    23401 gcggagggtt atcaccagag cttgatcgac attactcccc agcgcgtact ggaagaactc
    23461 aacgcgctat tgttacaaga ggaagcctga cggatgcggg ttttgatcgt taaaacatcg
    23521 tcgatgggcg atgttctcca tacgttgccc gcactcactg atgcccagca ggcaatccca
    23581 gggattaagt ttgactgggt ggtggaagaa gggttcgcac agattccttc ctggcacgct
    23641 gccgttgagc gagttattcc tgtggcaata cgtcgctggc gtaaagcctg gttctcggcc
    23701 cccataaaag ctgaacgcaa agcgtttcgt gaagcgctac aagcagagaa ctatgacgca
    23761 gttatcgacg ctcaggggct ggtaaaaagc gcggcactgg tgacacgtct ggcgcatggc
    23821 gtaaagcatg gattggactg gcaaaccgct cgcgaacctt tagccagcct gttttacaat
    23881 tgtaagcatc atattgcaaa acagcagcac gccgtagaac gcacccgcga actgtttgcc
    23941 aaaagtttgg gctatagcaa accgcaaacc cagggcgatt atgctatcgc acagcatttt
    24001 ctgacgaacc tgcctacaga tgctggcgaa tatgccgtat ttcttcatgc gacgacccgt
    24061 gatgataaac actggccgga agaacactgg cgagaattga ttggtttact ggctgattca
    24121 ggaatacgga ttaaacttcc gtggggcgcg ccgcatgagg aagaacgggc gaaacgactg
    24181 gcggaaggat ttgcttatgt tgaagtattg ccgaagatga gtctggaagg cgttgcccgc
    24241 gtgctggccg gggctaaatt tgtagtgtcg gtggatacgg ggttaagcca tttaacggcg
    24301 gcactggata gacccaatat cacggtttat ggaccaaccg atccgggatt aattggtggg
    24361 tatgggaaga atcaagtaga gtgtcgttct acaagtatgt ctcttgcaga tttgccagct
    24421 caaaccgttt ttcaaaattt aaatcttgaa ataataacca ataagttgac atcggagata
    24481 agatgacctc aacattattt ttctctctcg agaaaaaaaa ctggatagcg tactggaaca
    24541 gagctctcgt attcttattc attaccacct attttttggg tgggattaca aggtataaac
    24601 atcttattgt tattcttatg acaataacga caatcgtcta tctctgcaaa cggccaaaac
    24661 actatctctc actatttaaa acatttcttt ttggtagtgt tgccatatta actattgctg
    24721 cattgctgtc acttcttcaa tcccctgatg caggtgctag catgaaggaa attttcaaag
    24781 ctattattga gaatacttta ctatgcacaa tagcaatacc ggtcatattg agagacgaga
    24841 aaagagaaga tgtcgaaaaa atcgttttct tctcatttat tagtgcgttg ggcttacgct
    24901 gtttttctga attgattacc tattataagg actatcaaca agggataatg ccattcgcag
    24961 attatagaca ccgtagcatt tctgactcga tggtcttttt attccctgca ttgttaaatc
    25021 tctggcttat caaatcagca aaataccgca tttcttttgt ggttctaagc gttattttta
    25081 tttttctgat attaggaact ttatccagag gggcctggct ttccgtgtta gtcattggat
    25141 taatatggat tctgatgttt aaacaatgga agttactatt agtaggagta atggtttcca
    25201 tcattgcatt gtcggttatt ttcacacata aggagatgac cgcaaagcta acgtataaac
    25261 ttcaacaaac taatagttct tatcgctatg caaatggtac tcaaggcagc gcactcgatc
    25321 taatattaga aaatcctgtt attggttatg gttacggtaa cgttgcatat aaagatgtct
    25381 ataataaacg tgtcattgat tatccagaat ggacctttag acaatcaata gggccacata
    25441 attttgcgct attcatctgg tttggcactg gtttattagg gctggtaagt cttatgatgc
    25501 tatactgtgc aatattgaaa gagtgtataa aaaatggcgt caagaataaa tatcgctcac
    25561 catataatgc atattatata atcttactat cttttatagg ttattttgtt atccgtggaa
    25621 acgtagaaca aattgaacca aatttattag gcgtttacgc cggcttatta ttagcgatga
    25681 aaaacaagta aaaaataaaa aaggctgcat aatgcggcct ttttatttat tcaaaccaat
    25741 tatgaataac ctcttcgaat ctttgagtta cgccatccca actgtagtga tcaaacacaa
    25801 agtcctgacc ttgctttgca actgctgtta attcaggatt cgctaacgtt tttaaaatat
    25861 cgctgcttat ggagtctgca gtcataggtt cttttaaatg gaatcccgta gtattttctt
    25921 tgacaaattc cgtcatcccc ccacgcgtac tcactaaaac aggcttacca gcacccatag
    25981 cttcaattgc caccatgcaa aatggttcct ggaactggga aggaatgacg actaaatctg
    26041 ccagtgggta ataacaatgc atcttttcag gcggtatgct tcctaacata atgcaacgat
    26101 ccttcaaacg ttttgcaatt tccctgacat tgcgttgata tgcacctttg tcacttttac
    26161 tcatttctgt ataatcacca atcacaacta attttaaatt tttgtgagca gcggccaatt
    26221 tttcgaatga ttgcatcaac agcaatatgc ctttgtcagg gacgatacgc ccagcataaa
    26281 aaataatttt ctcttcaggg gagatattaa gttcgcttct agtgatcggc tgaaaattag
    26341 attgataggt ttctaaatca attccatttg gtacaatctc aatatctgca tttgctaaat
    26401 aagattggta atactttttc aagtataaac tagggactat cattttaaca ttttgctcaa
    26461 gcaattttgg ttcaaatgca ttatgcatat gtataaccac tcttgcctga ggagctcgct
    26521 tacgaatttg agtgtatagc ttcatgctat tatgaactac aataacactt tctttcgtta
    26581 taggaaaatc atgtgcaata ttcaatattc tctgtgaata tggaagagga tcaagtcttg
    26641 tccatttttg aaataaccgc ttatatatgc gactgaatcc aaccctatga atactacaat
    26701 tatcattaac aaaggtgtaa tttgagtatc ctggattttt gatacaaaca atacgatttg
    26761 gaaaattggt acgctgagca acctgataca tccacgtttc aacagcagcc gctccgcgag
    26821 gaggaattga aaaaatagga gtaaccgtaa atattatttt atcaaccatc tcgtatatta
    26881 acctttcata acattatatt taattgctgt aaaaacacca tcgagaaatt ttttctggta
    26941 aagcaagtgt ttatattttt ctttgtgctc atgtttttta acagcttttt tataaggtat
    27001 atttctccat ggtgatatat tataaatatt acgaaaataa tctgcagagg cgtagttcgc
    27061 ccaatcatgc catggcttag ttataccagt ataatgtatg aagacagtgt catcattaat
    27121 aaaccgggtg taatattcac tatttttttc ttcaaattct gactttatag tataaataca
    27181 attatatttt ctcggtaata ttttagcatg atgtagaaac attaagttta aaatatcttg
    27241 atccgggtac tttaattttt ttataattga ctcatctgat aatagatcaa agaatttttc
    27301 cgttagtctt tgttttaacc attcccgtaa attaatatac attacaccag agttaaaata
    27361 gctaccgtta aaatcctcat tacacaaacg ctcagcgctt ttactttgca tagcatctac
    27421 atcaattacc actgccccat attcatcttt aaactctaat gctattaact cgttcaatga
    27481 acccttacag acgatatcag catcaagata taataatgaa tccaatcgcg cagaaaaata
    27541 atcaaatgaa agcaaacgat aataaataga tactggccag atatttgatt gaggtagcgc
    27601 cttcaatggc tcagaatcaa tatgatataa tttaattact gtttgatact tcgttgctaa
    27661 ttgtgataat aattcaatat actctttgtc taagtaatca gcgattatgt gaaaggtaaa
    27721 tttgttttgt ttattctttt caagaattga agcaattgaa atcgcagccc caacctggta
    27781 gttctcatca ataccccatg agacattaaa cgtttcccgt tcatcaagtt taacggggcg
    27841 cttgtccagt tcaattatat ctttaaattg agtaagatgt ttaaaatcca atttaattac
    27901 ccttcatcat aatcattatt tgctcttcaa tcctagtttg acttttacat ccctgattac
    27961 ttttttaatt ttctttttga atataagata tgtatagcca aagtctttca attcattttt
    28021 tatattatag tgccgttcaa gatcaatgcg atcttttgct tttaaaacag cggttgtttt
    28081 tttacctgaa aggtcaatta gtcttaaacc tttttcggat actataaaat ttcctttatg
    28141 aggatcgcca gacaccattc catgctgatg caactctttt atcgattctg ataattgatc
    28201 cttcagatct tcagaaattt ccaaatactc atttaacccg acaccttcaa tatactcaat
    28261 gagcataata taataatgag cgaaatttaa tgttttacgt tcagctagca aaaaatagtc
    28321 attaattgat tggatgcctt caccacgtac ccgatcggtt tgataaatta aattttcata
    28381 ataatctttt tttatgcaag attttaagaa acgttcggtc attttatgct ttggcgcata
    28441 aactttaagt acaagaggtc cacgagcagt gtcaataaga atgactttag tatcatcaat
    28501 agaacgaaaa acttttaatg ttttaaccct gcaagttaaa aagtcattta gaacttgctc
    28561 ataaaaggga tcattatctt ttatgaaaac tttcaaccca tttatcgttt tattatatat
    28621 cataatgaat tattttaacc ttaaatcttt agaagcattt ttctttataa tactttaaat
    28681 aattaaaaat gcctgccata taatgctttt gtttaaattt atgttttgca caataccgat
    28741 actgattgct atttacaggt ttaagtaaat cttctttact ccacggagaa gctgctttgg
    28801 cgatcaaaaa actacgtgaa accggataat cagcccactc atgccagggt tttgtaggtc
    28861 ccacatagtg tataaacacg gtgtcatcat tgactggatt atcaactttg tcttttaatt
    28921 catagttaat actatatcgg gtattatatt tctccgaaat aaacttcact ttaccattca
    28981 ataatacatt cagtacatct tgatcaaggt gggtgatttt acttacccaa tctgggtcac
    29041 gcaacatttc aatagctttt gacgaaatgt tatttagatt ccactcatca atatttatca
    29101 gtaaaaaacc agcattgaaa tatccagaag ctaactgtgg tgtagttaat accgaggctc
    29161 gattttgcca ccattcaata tctctttcag ctacaactgc ggcaatttca ttagtagaaa
    29221 attgataatc taagagttct ttaatactac ccttgcaagc aatatctgca tcaagatata
    29281 gtattttttc atgtttatga tagaaataat ctgcaattat aaatcgaaaa tatgttgcgt
    29341 aagtccagtt tttcgtgctt ggtaatgact tcagcttatc acaattgata acataaatat
    29401 taattcttga attatattgt tttgctaaat cagaaaaata taatttgtct ttatcactga
    29461 tataatctgt gaaaacatga aattcacaag agatttctct attgtttaat agaatagatg
    29521 cgatggctac accacatcca aaaagaaaat ttctgtcaat accaaatgct atatcaagag
    29581 tatttttaga tcttatattt tgataatgaa aattatactc aaaaaggatg atgtcactat
    29641 cattgagttg agacatattt attctgtatc caaaattttg gcaaaaataa gttacgggtt
    29701 acaacgattt tcgaatcgtt ctttccctga tttttgtggc ttttgcttct gcttgcgaca
    29761 gcagtccttg ttcctgcttg agaatgtctt taagcggggc ggcaaaatac accttcataa
    29821 agcgccagat atcccgctga gtcaggccaa tattcattga agaaaaataa agccctataa
    29881 gatctttatc gcgccaacga cctggaacgc gcgtgcgaag ctgcgcccgg tgtaggtcaa
    29941 ttaccgaaat ttttaactcc tcttccttac cggaaaaagg caagtgcagc aggaaatgac
    30001 agatataaca gtcacgatgg ttaatgcccg cagcatgcat atcgcgcacc atcgtcgcga
    30061 cacgcttaat aagcatacgc tttacgcgaa catctggtgg gttggtcgcc cagtcagcac
    30121 agtaatcttc cagactgatg gttggtgtca gatcttcggt aataataaac gaggttctgg
    30181 tcagcggatt catgcctttt tcgccgaatg ccaccccata catagtatca acgccgacat
    30241 cccgcagtcg atgaatcgca ttccattcgc ggtctgcgcc taatactggc atccgcaatg
    30301 agagtaaatt tttgattatc tctttcaggg tcgtgccgcg atgccatttg agaaaatagc
    30361 tttttcccgc catttcaaag cgcagagtac ggcgagtttc cagttcacga aatacctcac
    30421 cctgcaaggt tttaacttcc tcaaaaggat ctttgccgcg ccataacgtg gcaaacggct
    30481 ctttaagttc aaccatccag accacccgtt atgatatccg ccgctttctc tggcagactg
    30541 tataaatctt gtgtatcagc ataatgtcgc gcattttccg cccaagcctg gcgcaatgaa
    30601 gattgcgtta acgctttgcg taaaatctca ttcaatgttt cctggcggaa tggctcagca
    30661 atagcctcgc cgcaattagc gtcgacaata taatgcgcat agccacaaac ggcagttgtt
    30721 agtaccggta atcctgcagt aatcgcttcc agcagcacaa ttcccgccgc ttcctggtag
    30781 gcaggatgca gtaataaatc cgccgccgcc attaattccg agacatcgtt gcgccccgag
    30841 aagaagtgaa cattactgcg cacgccgcgt ttttctgcca gtgcctcaaa ttttcgcggt
    30901 ttatcctgcc caacaacata tagcaatgtg ttgtggcgca gcgaatccgg taacgaagca
    30961 agtgcttcaa tggaacgatc gacaccttta cgcgtgaagt ctgaaccgac ctgcaacaat
    31021 aaatattgtt gttcggttat tccattcttc ttacggaaga tttcacggct attggctggc
    31081 tgctggctat atttacgatc aggataaatc cccggtggca gaatatgaaa acgctccgct
    31141 tcagtctgat aatgtttctg gaaatcggcg atttgcttat ctgtcagcat cagcagctgt
    31201 gtcggcttgc cctgttcgaa ggttgcccgc tcaaaggcgg cataatggcg ataacgtgac
    31261 gtcaggcgat agaaaaagcc tttttcctgc gctactttct cggcataaca aacatcagcg
    31321 gcataataaa cgtccagccc cggcattttg ttgaatccaa cgactttatc gacgggatgt
    31381 tcgcgtaaat gtttttgcac ccaggcaaaa tactccgcat tgcgcccgtg attggtatgc
    31441 gatttaaccg gcactttgat cagttcaaat acatcagggc attcgccttc ccacgactgg
    31501 gtataaaccc gaacatgatg acctcgggcg gcgactgtct gagcaatacg cataaaatcg
    31561 cgctgcaaac cgccaaaggg aaaatattta tataaacaaa aagcaacgat cataacgacg
    31621 tacctgtcgt ggaggagggc agtaatttat cgacagcagc aatgacatct gccgccggaa
    31681 taaccgaaag atacatctca tttcggtcac gctgatcgcg cgttggcatt tcccggtaat
    31741 ctcccgccca gaattgaatc atgttatttg accagggacg ccagaaaata tgatctgttg
    31801 cgccaaacag cgatatcagc ggcgtattaa ctgcagcggc aatatgcgcc ggtgcggaat
    31861 caacgccaat aaacagctgc gcatgatcga ttaacgcacc aagttccggg aaggtcacct
    31921 ttccagccag cgccgttact ggtggcgtct ggcatccctg cgcaatttca ttgacgcagg
    31981 ccagatcgtc tttatctggg ccggacgtca gaacgacttc ataaccacga gcatgtaagg
    32041 catcaatcac agcggaaaac ttggcgttgt cccagcattt gaagatttgc cgcgccgtag
    32101 gttggataac cacataattt tgtccaacac cagcgtgatc aagttcgcga cgcatacgtt
    32161 tccagcttgc aggcgggtaa ctcatggttg tctgcttcac caacgaatca agtcccaatg
    32221 gtgtcagcac ggataagtta ctttccacca catttccacc ctgcaacggc accaaatggg
    32281 tgaaactttt acgccaaaaa gcagactgcc gatgatgata atcctgggaa attttcacac
    32341 gagcatttaa taagcgaacc agtatagcaa ccatccattg atcggtaaga ttgacgataa
    32401 ggtcatactt attggcacgt aataccttga tgagatgaaa aaagttggca attttttctg
    32461 aggcttttgc ttttttattt tttatgccgt agagcgcgtt aatctctgga ttttcagaca
    32521 ggatcgggat ggtgtcctga taaagcagca catcgatttt tgcgtcaggg taattttttt
    32581 tcagagaact aatgacggga gtagttaata acatatcccc atgaaaacgc atcttaatga
    32641 gcaaaattct tcgaaatggc ttatccacaa gtaactcttt ttgttaagat tatccgatta
    32701 aggtgaagca gaaaaagcac gctaccgccc caggctcaac agctacctga atactgatac
    32761 cgcactgata ttgacgcgct agtgtatcac ttctttcgcg gcaatccgac ccgaatgatt
    32821 tttaagcaca ttgttgattt tcaataaaga aatcaatcaa ataaaatcat tagcaaccat
    32881 attctttcac tgttgctctg cgattccttc ctgcacgtac agcagaaatc aatgaatcgc
    32941 ataaaaatac aaaaaaagta tagatgttca gcaagaattg gcgggtaaat tgctatttaa
    33001 gtcaaaa
//
LOCUS       DADMGC010000031        32927 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.031, whole genome shotgun sequence.
ACCESSION   DADMGC010000031 DADMGC010000000
VERSION     DADMGC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.031"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..326)
                     /gene="yfdR"
                     /locus_tag="JNZ73_001438"
     CDS             complement(<1..326)
                     /gene="yfdR"
                     /locus_tag="JNZ73_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY8731764.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKQMEEKIDSVIR
                     EKYGLPP"
     gene            complement(454..1278)
                     /locus_tag="JNZ73_001439"
     CDS             complement(454..1278)
                     /locus_tag="JNZ73_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HAY8731765.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDTIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCIPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            complement(1344..1724)
                     /locus_tag="JNZ73_001440"
     CDS             complement(1344..1724)
                     /locus_tag="JNZ73_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731766.1"
                     /translation="MMRSSKMASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMN
                     TKTKGKVSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTI
                     LQEDQGQLLTLAGEPDGKLRAAGH"
     gene            complement(1721..2031)
                     /locus_tag="JNZ73_001441"
                     /pseudo
     CDS             complement(1721..2031)
                     /locus_tag="JNZ73_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2307..2603)
                     /locus_tag="JNZ73_001442"
     CDS             complement(2307..2603)
                     /locus_tag="JNZ73_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731767.1"
                     /translation="MLKQVYEMKVQILNNNCEVVWSYDIAAPVDQSGDSWTNGKHQIM
                     AGVVFSLRRALEQAEVFPSDPEWKWPFSICPNSESTFQKIGQKVALEEHQPTVS"
     gene            complement(2776..3450)
                     /locus_tag="JNZ73_001443"
     CDS             complement(2776..3450)
                     /locus_tag="JNZ73_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HAY8731768.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            3541..3741
                     /locus_tag="JNZ73_001444"
     CDS             3541..3741
                     /locus_tag="JNZ73_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY8731769.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            3785..4336
                     /locus_tag="JNZ73_001445"
     CDS             3785..4336
                     /locus_tag="JNZ73_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731770.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDEQYLSISKLQEHAALVYKIFCVSE
                     SSDARECAAPGAVACRDCGETNA"
     gene            4512..4691
                     /locus_tag="JNZ73_001446"
     CDS             4512..4691
                     /locus_tag="JNZ73_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003034732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY8731771.1"
                     /translation="MRQVNRWFKEHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            4681..5622
                     /locus_tag="JNZ73_001447"
     CDS             4681..5622
                     /locus_tag="JNZ73_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY8731772.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKTSRPDASQP
                     DTQTAEQEFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            5619..6113
                     /locus_tag="JNZ73_001448"
     CDS             5619..6113
                     /locus_tag="JNZ73_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HAY8731773.1"
                     /translation="MSMSLLNDVQKFIEAHRGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            6113..6766
                     /locus_tag="JNZ73_001449"
     CDS             6113..6766
                     /locus_tag="JNZ73_001449"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HAY8731774.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPVWFIPKDE
                     KQVLTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            6763..7089
                     /locus_tag="JNZ73_001450"
     CDS             6763..7089
                     /locus_tag="JNZ73_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAY8731775.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRATHWLH
                     ERDLKV"
     gene            7086..7475
                     /locus_tag="JNZ73_001451"
     CDS             7086..7475
                     /locus_tag="JNZ73_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY8731776.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITINKYEKTAGAAA"
     gene            7495..8292
                     /locus_tag="JNZ73_001452"
     CDS             7495..8292
                     /locus_tag="JNZ73_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HAY8731777.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSQDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVSRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            8300..9289
                     /locus_tag="JNZ73_001453"
     CDS             8300..9289
                     /locus_tag="JNZ73_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY8731778.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            9304..9672
                     /locus_tag="JNZ73_001454"
     CDS             9304..9672
                     /locus_tag="JNZ73_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
                     /protein_id="HAY8731779.1"
                     /translation="MMRDIQMVLERWGAWAASDSSGVDYSPIAAGFKGLLPYTSKKRL
                     ACSDSDALIIEGCLARLKQKRPYEHSLLVAHYLYRISKRKIAKARGKDEKLIRIEIQL
                     AEGFIDGCLSMLDLTLDLDV"
     gene            complement(9701..11032)
                     /locus_tag="JNZ73_001455"
     CDS             complement(9701..11032)
                     /locus_tag="JNZ73_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HAY8731780.1"
                     /translation="MLDTLSFTERDEFQRRNIAENIIKLLKPEADISPLVIDGAWGTG
                     KTEFSIKLKNLIIEQETESKVVYIDAFKGDHAESPLLLITSAIASILPEEEKQHFIKR
                     SLPAIRFGLKTVLKAGAGWFLRQEASEVSEEFQDAMKKASNAAIDGTIENLLEDHMES
                     EKNINSLKSCIESISESQKIVIIIDELDRCKPSFSTNVIETIKHIFDINNVFFILVTN
                     TEQLKASINHIYGYSINSQKYLDKFIKYTITLPDTCLINGHVVCKTSVIYWNYLVDKT
                     KSLNEINRLSGRFIRDLIQRTNLSLRETQTFSRNLNIFQLLNDDEYKSSDPLINKIFV
                     VAVFIHCFGDKEKLKQEITAESISHLADLLNIKEIPYSYERRSQIPEISIVFFGIIKD
                     SITLNERFAPKSDEELKKFTTVYTDYEHLNFWSTTPRELLIKYINQMSFIQ"
     gene            11329..11655
                     /locus_tag="JNZ73_001456"
     CDS             11329..11655
                     /locus_tag="JNZ73_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HAY8731781.1"
                     /translation="MPHNPNTWLELVQSWWRGDTPLGAVIMSIVMAGLRIAYFGGGGG
                     WKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMRVIGNK
                     FGGSKE"
     gene            11659..12135
                     /locus_tag="JNZ73_001457"
     CDS             11659..12135
                     /locus_tag="JNZ73_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HAY8731782.1"
                     /translation="MQVLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLALKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLIARFKEAGGVVNEAEL"
     gene            12119..12511
                     /locus_tag="JNZ73_001458"
     CDS             12119..12511
                     /locus_tag="JNZ73_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HAY8731783.1"
                     /translation="MKLSYKLLIVGFFVTVIGSFIWSANHYYSKYQYEKKRADEAVRN
                     AESATAITNNVLQSLQIVNTVLEANQHAKQQIALESQRAREDIKVAVADDDCASRPVP
                     DVAADRLRKYADSIRAGSNDAVTDEPAR"
     gene            12974..13306
                     /locus_tag="JNZ73_001459"
     CDS             12974..13306
                     /locus_tag="JNZ73_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731784.1"
                     /translation="MPVNIDPEKLNDEREQVIAKWLFKDVDLISQQIELGEENVKRFD
                     ELLSIFDCCQSSWFATEHLFDNTELEKVWHEFESNFNKYINGGESKDLLMKMLDKLIS
                     SRFVFESR"
     gene            13357..13707
                     /locus_tag="JNZ73_001460"
     CDS             13357..13707
                     /locus_tag="JNZ73_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY8731785.1"
                     /translation="MPPRIPKACRVRGCRSTTTDPSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDVIRVRVLKRDKGLCQLCLRAGVVREAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKEKTARERLK"
     gene            13833..14327
                     /locus_tag="JNZ73_001461"
     CDS             13833..14327
                     /locus_tag="JNZ73_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003832359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY8731786.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEELPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            14561..16057
                     /locus_tag="JNZ73_001462"
                     /pseudo
     CDS             14561..16057
                     /locus_tag="JNZ73_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005147477.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            16069..16251
                     /locus_tag="JNZ73_001463"
     CDS             16069..16251
                     /locus_tag="JNZ73_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731787.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQSSVGGGK"
     gene            16251..17492
                     /locus_tag="JNZ73_001464"
     CDS             16251..17492
                     /locus_tag="JNZ73_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY8731788.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADDIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRATGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPSCVVPKLNSSWEPVYQVTFPDGSADVLS
                     QEDIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            17470..18120
                     /locus_tag="JNZ73_001465"
     CDS             17470..18120
                     /locus_tag="JNZ73_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAY8731789.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRTKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYIALSLRDAEDVGSALNALKNLNF"
     gene            18135..19340
                     /locus_tag="JNZ73_001466"
     CDS             18135..19340
                     /locus_tag="JNZ73_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY8731790.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILTLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDTRSQTATSRLELIEPLMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTTGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATAVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDSEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            19390..19590
                     /locus_tag="JNZ73_001467"
     CDS             19390..19590
                     /locus_tag="JNZ73_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731791.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKATKEKKATTSKEG"
     gene            19593..19916
                     /locus_tag="JNZ73_001468"
     CDS             19593..19916
                     /locus_tag="JNZ73_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY8731792.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            19913..20323
                     /locus_tag="JNZ73_001469"
     CDS             19913..20323
                     /locus_tag="JNZ73_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY8731793.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRLRVDMPADNFGVEPQYL
                     ITFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCGDSVYRVKRQR
                     DLNGARRFLLLECTELGECGQSHGGNNDDFLFAR"
     gene            20298..20804
                     /locus_tag="JNZ73_001470"
     CDS             20298..20804
                     /locus_tag="JNZ73_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731794.1"
                     /translation="MTTSFLHVDFQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKHRPGFMARIAPNQRNGEGNRRITGDFYP
                     AFLFYGVRRGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            20801..21361
                     /locus_tag="JNZ73_001471"
     CDS             20801..21361
                     /locus_tag="JNZ73_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731795.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLRLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELRTLA
                     IDVDYLEPGNGPDGDIEHHTEITLPS"
     gene            21370..21537
                     /locus_tag="JNZ73_001472"
     CDS             21370..21537
                     /locus_tag="JNZ73_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HAY8731796.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNEKVNTDDEL"
     gene            21524..23017
                     /locus_tag="JNZ73_001473"
     CDS             21524..23017
                     /locus_tag="JNZ73_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8731797.1"
                     /translation="MTSFNTIPSNTLVPIFYAEMDNSAANTAQDSGASLLIGHANNGA
                     EIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPEATGAAATVT
                     LTVTGEATESGTVNVYVGRTRVQAPVTNGDNVTTIASSIQDAINAVPALPFTASSSAG
                     VVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAAM
                     ADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAGD
                     QFNQQHITLAGYEKETQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLPAPKGK
                     RFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAYV
                     LRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYEL
                     FKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            23017..23373
                     /locus_tag="JNZ73_001474"
     CDS             23017..23373
                     /locus_tag="JNZ73_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8731798.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNRTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPASKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            23373..23642
                     /locus_tag="JNZ73_001475"
     CDS             23373..23642
                     /locus_tag="JNZ73_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HAY8731799.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            23784..25619
                     /locus_tag="JNZ73_001476"
     CDS             23784..25619
                     /locus_tag="JNZ73_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY8731800.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGSAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETISPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYIPYPAVDLAQSNNASDATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            25680..27008
                     /locus_tag="JNZ73_001477"
     CDS             25680..27008
                     /locus_tag="JNZ73_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HAY8731801.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEENAGTGRRVETHEYPNRDKPYTE
                     DLGKVTFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKGEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNASTMLGYVSDAMKVVDSAVSDVARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNAAVEQATTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            27005..28084
                     /locus_tag="JNZ73_001478"
     CDS             27005..28084
                     /locus_tag="JNZ73_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HAY8731802.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEISRQWPGD
                     GGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRELVQIAQALAAPFGIEVVNNGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEDPGARKKKKARVQEDPF"
     gene            28084..28632
                     /locus_tag="JNZ73_001479"
     CDS             28084..28632
                     /locus_tag="JNZ73_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HAY8731803.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCLTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPEARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            28632..29057
                     /locus_tag="JNZ73_001480"
     CDS             28632..29057
                     /locus_tag="JNZ73_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8731804.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYASRLWLLQRSKLTNQLVQTVRGYIRECLQWMTDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            29044..30102
                     /locus_tag="JNZ73_001481"
     CDS             29044..30102
                     /locus_tag="JNZ73_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HAY8731805.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDEFWLARHAAMKRCPRKGATAASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSSGGVLRLPIACSTAGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEDLETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWAY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHTVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGADGEYSHQLLAPVD
                     NISIAKNELAVLGTISWT"
     gene            30093..30677
                     /locus_tag="JNZ73_001482"
     CDS             30093..30677
                     /locus_tag="JNZ73_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HAY8731806.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSSSDPAIAGAAPSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPAGIQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRQDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNSTWMTCGD
                     PCDSALRIWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            30681..31331
                     /locus_tag="JNZ73_001483"
     CDS             30681..31331
                     /locus_tag="JNZ73_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731807.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            31303..31737
                     /locus_tag="JNZ73_001484"
     CDS             31303..31737
                     /locus_tag="JNZ73_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HAY8731808.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYLTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(32510..>32927)
                     /locus_tag="JNZ73_001485"
     CDS             complement(32510..>32927)
                     /locus_tag="JNZ73_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8731809.1"
                     /translation="GFIVFSITAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAIN
                     TIITTVGLIIFNIFIAKIICGNDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVT
                     IPNPICAIIYTCIYVYVSDKIGHWLSRTVGGPFLIK"
BASE COUNT         8138 a         7744 c         9104 g         7941 t
ORIGIN      
        1 gggggtaatc cgtatttctc acggattacg gagtctattt tttcttccat ttgtttatag
       61 tcaggaagaa ggcgtttcag tggtgcggga atgtcctggc aatacgcttc tgttgcatca
      121 tgcattaacg cttcaaaagc aaattcctgc ggcaccagct ggctgcaaag aaccgcatgt
      181 tgggcgacgc tgtagaagtg cgaaagatga ccggcaaagc gacagatatt tgaaagggaa
      241 accgcgatat cgttaatatc gatgtcgtct ttatttatcc tgtcataata aaaatgcttc
      301 ccggaaaaag ttttaataaa tgacattttg ttctccacgt atatgcgctg caccgcgctg
      361 aattctggta aaaagaatcc ctcaccatcc ggcgattatt gagtaaatta cgtttccata
      421 aatgcccccg caggggcatt tgcagtaatg aaatcaggcg gtgaaagtac caataaaggt
      481 ttctactttg ctgtccttga atttctcaac aagcagatca cgaaattcgt tagccatttc
      541 ttcctgcacc gcctccagct gaataatgcg cagaaccagt accggacgat cgccagtgat
      601 aatgctgagg cgtaatttaa acggacgttc tttcagacct tcaaacggaa tgcatttaaa
      661 ttcaaatgcc actggcataa tgtctttggt cttcgcttcg acagactcca tcagggagcg
      721 tttgccgctg aagtcattat cttcaaaatc agcggtctgg tttgcttcaa tcgtgatttt
      781 acggaccgcc gcagccgctt ttgttgcctg aatggtgtca ccattagcat caaagcccac
      841 aaggtagtcg gcccagtctt caatccattc tgccagtgat ttctgggagt tacgctcgcc
      901 attaacagac aacagagcag agaacggtgc tgtctttttc agtttgagag tggcggtgtt
      961 atctgcgtga cctggttcat caatagtacc caggttaagc acactgacgg cacgcatatt
     1021 atcagcatcg ataaagcagc gggtgccttc atctgcaaga tctttagaat aacgggtaaa
     1081 gtcatcgatg ctggcagtgg aaagcgcacc acggaaacgg aagcgattta aattaaattt
     1141 ttccagatca tgaatgcgga aattctcagg caatgccaca gcatcggcac caatcttact
     1201 gataatttca ttaacaccct gagcagaaat aagggcatgg atttgattaa ttgcggttgc
     1261 gtctaagttc tgagacataa taagtcctaa ctatataaag atattcagtg atgagataaa
     1321 taatcagtta attaagaacg atattaatga cctgctgcgc ggagttttcc gtcaggttca
     1381 ccggcaagag tcagtaattg tccctggtct tcctgcagaa tagtcaggcg accaccgcga
     1441 ttgacataca tcggcgtttc ggtggtgtct tcttcggaaa ttttcccgcg gttagtcggg
     1501 cgaacatatg agagtttgtg tttgattttc acacggttct catcaaatgg ttcgatttcc
     1561 aggttgagtg agaccttacc tttggttttc gtgttcatca caccggaagc gacttcactg
     1621 agaactgcgc cgattttggt ttcaaatacg ccgccgtcca gctccccgat aaatgcctgc
     1681 acatcagtac tgcgttcgct agccattttg ctgctcctca tcatatcgac cctgcaaggc
     1741 cgattagttt ctccacaaaa cagagaagaa cacctgcggt agcagccgcc cggatggatt
     1801 gggttatgag cccgtcgtcc ggtgatgctc ttctctgttt tgtaaaaagg acggtaccag
     1861 ccggaagcaa gggtacaaac tggtaccgcc aggactacac acagcataaa gttgtggtgc
     1921 cgggtgcctc ccggtgcctg gcgaaggttg cacaccagac gggtgggtat ccacagaagg
     1981 tcgactgtca gcctcaacct taacccgcgt gcgctgagcc gcattcacca caactctaag
     2041 gattctcttt ggttgaaaat acttagctgt tatgtgcctg tcttttcacc acttcaggct
     2101 cggtggtatc cttttaagcc cgtatacata aaaggaaaat caaatgactt ttgatgaaaa
     2161 agaacttgat aatgcaatta ataaaatcat cgtaacgtcg ctcttttcct gtctcagcga
     2221 cactcagcag aaacagttct acgaatcggc tttcaacatg atcgagcgtt gttgtttctg
     2281 cgatgccgac gagttacctg aaaaaatcag gaaacagttg gctgatgctc ttcgagtgcg
     2341 actttctgac caattttctg aaatgtgctc tccgaatttg gacaaataga aaaaggccat
     2401 ttccattcag ggtctgatgg aaatacttca gcctgttcca aagcacggcg taaagagaac
     2461 acaactccag ccataatctg atgtttccca ttggtccagc tatcgccgct ctgatctaca
     2521 ggggcggcta tgtcgtatga ccaaacgact tcacagttat tgtttaaaat ctggactttc
     2581 atttcataca cctgctttaa catgagtgcc tagtggcaca acatgactca acgaatcatc
     2641 ctggacttca tatgccccag gcggctactt cgtgggcgtc ctgcctgttc gttgtttcgc
     2701 ttgggtacat tatgtatccc aagggtacat tgtcaagtat aaaaaaacct gccgaagcag
     2761 gttcataaac attgattagg ctttgatttt gtatcttctt ggttttcctg agaaaatcac
     2821 tgtaccaatt atagagcaat taccgttgat cttaatgtaa ggctcaggcc agtttgggtt
     2881 taacgctttg agataacgct gtgtcccatc ttctatcaac cttttgaagg tggtttcgcc
     2941 tgtatcgtgc atcaatgcaa taacgtcgtc accgtggcag gcaggtactt caggatcgac
     3001 aaaaatcatg tctcccgggc ggtactcatc aatcattgaa tcacctatca cccgcaagat
     3061 ataagtcatt tccccacagg gtacagggca gggatacgtt tctgctgtgc tcaaatcaac
     3121 ctcagaatat ccaacttctt tccatgctcc ggcctgtacc catgatatga cagggactaa
     3181 tgtgatttgt ttattagtga ttgaaacatc aggttttttt gtgatgttcg ttgtctggtg
     3241 ttcttgatcg agccatccga caggcaggtc gaaacatttt tcgatgtgtc gtgccatgct
     3301 gtcaccgata tttttagtag caccatctcc cataaacctg ctggtctggg ttggctcgcg
     3361 atcaatcata gtggcaaagg aagaattccc gccaacacca tctctcagtt ttctggcgtt
     3421 agaccgccgg atgtcatgga ttgttttcat aacgaaatta aaacccttgt accgttaagg
     3481 tacaagtgtc ttggaggttc atttcaatca tgtaacatgt acaccggagg tacatattgt
     3541 atgaaagcgt attgggactc tttaaccaaa gaacagcagg gcgagttggc cggaaaagtt
     3601 ggctcaacac ctggctactt acggctggtt ttcaatggct ataaaaaagc cagttttgtg
     3661 ctggctaaaa aacttgagca atgcacgtca ggtgcaatta cgaaatctga cttaagaccg
     3721 gatatctatc cgaaagatta gcagaacact ttcaattttt aaccacagaa cgatgaggct
     3781 aaccgtgggt aagcatcact ggaaaataga aaaacagcct gagtggtacg tgaaagctgt
     3841 cagaaaaact atcgcggcgt tgccgggtgg ttacgctgaa gcggctgact ggctcgatgt
     3901 aacagaaaac gctttattca accgccttcg tgcagatggc gatcagattt tcccgctggg
     3961 atgggcaatg gttttacagc gtgctggtgg cactcacttc attgctgatg ctgtggcgca
     4021 gtctgcaaat ggcgtctttg tgtctcttcc tgacgtcgag gatgtggaca acgccgatat
     4081 caaccaacgc ctgctggaag tcattgaaca gatcggcagt tattcaaaac agattcgttc
     4141 agcaattgaa gacggtgtag tggaaccgca tgagaagaca gcaattaacg atgagcagta
     4201 cctctcaatt tcgaagctgc aggagcatgc agcactggtc tacaaaatct tttgcgtttc
     4261 agaaagtagt gacgcccgcg agtgtgcagc tccgggcgcc gtggcatgtc gtgactgtgg
     4321 agaaactaac gcatgaacag tttaacaaca cactaccgtc gctcgcaact gattgcgctt
     4381 cctgtaccgg gtggaaaagc gaaggtggag tattgctatg cagtaaatgt accaggtgac
     4441 agggaaattg taacccacag ctttgcagag tgggctgtgg gtgatttcaa ccggcagaag
     4501 gagacagtcc tttgcgacaa gttaaccgct ggttcaaaga gcactacgga gtgcccgtca
     4561 gagtcattcg ttgggagccg gaaacacaac gtgttatcta cctccgcgaa ggctatgagc
     4621 atgagtgctt cagcccgctc gaacagtttc gtcgtaaatt cagggaaata gaggtcggtc
     4681 atgagcacta aattaaccgg ctatgtatgg gatggttgcg ctgcatcagg catgaaatta
     4741 tccagcgtgg caattatggc ccgcctggct gatttcagta atgacgaagg tgtgtgctgg
     4801 ccatcaattg aaaccattgc ccgccagatt ggcgcgggaa tgagtaccgt cagaacggct
     4861 atcgcacggc tggaagcaga aggctggtta acgcgtaagg cgcgtcgcca gggtaaccgc
     4921 aatgcgtcga atgtttatca gcttaacgtt gcgaagcttc aggcagcggc attttctcaa
     4981 ctgtcagatt ctgacccgtc aaaatctgac gcatcaaaat ctgacccgtc aaaatttgat
     5041 gcgtcgaaat ctggcaaaaa agcgggtttt cacccgtcag aatctggcgg ggatccgtca
     5101 gtaaaatcaa aacatgatcc gtcagataaa aaaacttctc gtccggacgc ttcgcaaccg
     5161 gacacgcaga cggctgaaca ggagttttta actcgccatc ctgatgcggt tgtattcagc
     5221 cctaaaaagc gccagtgggg aacgcaggat gatttgacct gcgcacagtg gctctggaaa
     5281 aaaatcatcg ccctgtacga gcaggccgcc gaatgtgacg gcgaggtggt tcgtcccaaa
     5341 gaaccgaact ggacagcctg ggcaaacgaa attcgcctga tgtgtgtgca ggatggtcgt
     5401 actcataaac aaatctgcga gatgtacagc cgcgtcagtc gcgatccgtt ctggtgccgt
     5461 aacgtgctca gcccgtcgaa gctgcgggaa aaatgggatg agctttccct gcgcttatcg
     5521 ccgtccgtca gcacgtacac cgaaaaacgc gaagacccgt acttcaaagc tagttacgac
     5581 aacgtggact acagccaaat cccggcagga ttcagggggt gagcatgagt cttttgaatg
     5641 acgttcagaa attcattgaa gcccatcggg gctgtacttc cggagatatt gcggatgctt
     5701 ttgccggtta ctcacggcag cgcgttctgc agtcagcaag caagttacgt cagagtgggc
     5761 gtgtggctca ccgttgtgaa ggagatacac gcagacattt cccgcgcctg actgagagag
     5821 cgcaggaacc ggaaccacaa ccagttcgtg aaaccagacc tgtgcgcaat ttctatgtcg
     5881 gcactaacga tccccgtgtg attttgtgcc tgacccgcca ggcggaagaa ctggagtcca
     5941 ggggcttata ccgtcgtgct gcaaccgtgt ggatggcggc attccgtgaa agccactccc
     6001 agccagaacg aaacaatttt ctggcgcgtc gtgagcggtg cttacggaaa agcagcaagc
     6061 gcgctgcatc gggtgaagag tggtatctgt cagggaatta cgtgggggct taatgagtaa
     6121 taaatattgc caggcgctgg tggaactgcg gaacaaacca gcccatgaac tgaaggaagt
     6181 gggcgatcag tggcgcacgc cggacaacat tttctgggga attaacaccc tgtttggccc
     6241 gtttgttctg gatctgttca ctgacggtga taacgccaaa tgtgccgcgt attacacggc
     6301 ggaagacaac gcgctggcgc atgactggtc agaacgtctt gcggagctta aaggtgctgc
     6361 ctttggtaat cccccataca gccgcgccag tcagcatgag gggcaataca tcaccggcat
     6421 gcgttacatc atgaaacatg ccagtgccat gcgtgataag ggcgggcgct atgttttcct
     6481 gatcaaagct gccaccagcg aagtgtggtg gccggaagat gcagatcata ttgcttttat
     6541 tcgcgggcgt attggttttg aactgcctgt ctggtttatc ccgaaagacg agaagcaggt
     6601 actgacagga gcttttttcg ctggtgctat tgctgttttt gacaagacct ggaagggacc
     6661 ggcaatcagc tacatcgggc gcgatgaact tgaggcatgt ggtgaggcgt ttctggcgca
     6721 ggttcgccag caggcggaaa aactggtcag ggagatggcg gcatgacgac gttaactcaa
     6781 tgccagcagc aggtgctgga tatgctgatt tcttatcaga aagaacgtgg cttcccgcca
     6841 accaatcagg aggtggcaac catgctggga taccgttcgg tgaatgcagc ggtggagcat
     6901 cttcgcgcac tggagaaaaa aggcgtcatc acgataaagc gtggcgtggc ccgggggata
     6961 acgcttcata ccgcggtgaa ggacgacgac agcgaggcgg tcgggattat ccgcgcactg
     7021 cttgccggtg aggaaaacgc aaggctgcgt gcaacccact ggttacatga gagggacctg
     7081 aaagtatgaa gctgatcctg ccttttccgc ccagcgtgaa cacgtactgg cgacacccca
     7141 acaaaggggc gtttgctggt aagagcctga taagcgcggc ggggcgaaaa ttccagagcg
     7201 cggcgtgcgc agcaatagtt gagcagttac gtcgtctgcc gaaaccaacg tcggcacctg
     7261 cttcagtgga gatcgtgttg tttcctccgg ataaccggat ccgcgatctg gacaactata
     7321 acaaggcgct gtttgacgcc ctgacccacg cgggtgtgtg ggaagacgac agtcaggtga
     7381 aaagaatgct ggtggagtgg gggccggtta tcccggaagg gaaggtcgag atcactatca
     7441 ataagtacga gaaaacggcg ggtgcagccg cctgattaag aggagaaacg aagtatgaat
     7501 aatctgatgg tcattgatgg tattgaagtt cgtcgtgatg cttatgggcg ttacagcctg
     7561 aacgatctgc acagggctgc cggttctcag gataagcata agcctgcatt ctggctccgc
     7621 aatgagcaaa ccgaacgttt aataagcgag ttgcagattt gcaactcggt caatatagag
     7681 ccagttaacg ttagtcgtgg cggaaataac caggggacgt atgtctgcaa agaactggtg
     7741 tatgcctatg caatgtggat cagcccgtca ttccatctga aggtgatccg tactttcgat
     7801 atggtaacca gcgcaccgga aaaattatcc gggcaggctg ctgacaagat gcaggctggc
     7861 gtgattctgc tggactttat gcggcgggag ttaaacctgt ctaactcatc tgtgcttggg
     7921 gcctgtcaga aactccagga ggctgttggc ttaccgaatc tggcaccacg ctatgccatt
     7981 gatgctcctg ctgacgcgcc tgatggctca agtcgcccta cgctgtcgct gagtgcactg
     8041 ctgaaacagt atggtatccg cctgacagct aatcaggcat atcaccagat ggcgaagctg
     8101 gggatcgttg aacaacgcga acgatacagc cgtaccgcga ttaacaacat caaaaaattc
     8161 tggtcgctga cggcgaaagg ctgcatgttc ggcaagaaca ttaccagtcc tgcaaatccg
     8221 cgcgagacgc agccgcattt cttcgaatcc cgattccctg agctgttaaa gctgctcgat
     8281 accgttcatt gaggtgaccg tgagagcact actgacccct gaaattgccc cgcgtatggg
     8341 gatcgtattg ttcagaccag gttcagagct gatgcccctg tttatgcagg ggcgtgtact
     8401 gctggagcct gagccggaac gttattcatc tttcgccagt ggtgccgttc ccgcggcatc
     8461 acaaccgctg gcggatgatc ctgctgttcg ggccgtgttc cgcaatgagg cagtgatccg
     8521 tcgtgctggt ggcgtggaat gtcttgaaag ctggctactt cgtgaaaaag gctgccagtg
     8581 gcctcattcc gactggcaca gcgagaacat gaccacaatg cgacacgctc cgggcgcaat
     8641 ccgtctgtgc tggcactgtg ataaccagct gcgcgatcag ttcacggaac ggctggaatc
     8701 aatggcaacg gataactgtg cccgctgggt gttgtctgtt gtgcgtcggg atctcggttt
     8761 tgatgatagt cacgttgtga caatgccgga actgtgctgg tggctgattc gtaatgacct
     8821 ggcggatgcc ttaccggaaa gtgcagcccg taaggcactg agattaccaa agcctgttgt
     8881 gccgtctgtc acccgggaaa gtgaccttgt gccttcggtt cctgccacca gcatcatcca
     8941 agataaggca aaaaaggtgc tggcgctgaa agtggatccg gagtcgccgg agtcttttat
     9001 gttacgccca aaacgtcgcc gctgggttaa tgagaagtac acgcgctggg ttaagacaca
     9061 gccgtgtgca tgttgtggaa agcccgctga tgatccccac cacctgatag gccacggtca
     9121 ggggggaatg ggtacaaaag cgcatgacct ctttgtgttg cctttgtgca gaaagcatca
     9181 cgacgagctg catgcggata ccgtggcatt tgaagagaag tatggctccc agctggagct
     9241 gatatttcgt tttatcgatc gtgcgctggc aattggcgtg ctggcctgat ttggtggaga
     9301 aagttgatgc gtgatattca gatggttctt gagcgttggg gagcatgggc ggcgagtgat
     9361 agttcaggcg ttgactattc acctatagct gctgggttta agggacttct tccctatacc
     9421 agcaagaaac gcttggcttg ttcggatagt gatgcattaa ttattgaagg ttgtcttgct
     9481 cgtctaaagc aaaaaagacc ttatgagcat tcgcttcttg tggcacatta tttatacaga
     9541 atatccaagc gtaagattgc aaaggcgcgt ggaaaggatg agaaattgat acgtatagaa
     9601 atacagctag ccgaaggatt tattgatggc tgcctttcca tgctagatct aacattagat
     9661 ttggacgttt aataatacgc cccatgcagg ggcgtattat ttactggatg aatgacattt
     9721 gattaatata ttttatcaat aactctctgg gtgtagtact ccaaaaattt aggtgttcat
     9781 aatcagtata aacagtggtg aattttttaa gttcttcatc acttttaggg gcaaatcgct
     9841 cattaagagt aatactgtct ttaattattc cgaagaatac aatagatatt tcaggaattt
     9901 gcgatcttct ctcataagaa taaggtattt cttttatgtt aagcagatct gctaaatgag
     9961 atatagattc tgcggtaatt tcttgtttta gtttttcctt gtcaccaaag caatgtatga
    10021 agacagctac gacaaagatc ttatttatta aaggatcact gcttttgtac tcatcgtcgt
    10081 ttaatagttg aaaaatgtta agattgcgtg aaaaagtttg tgtttcacgt aaggataagt
    10141 tggttcgttg aattaaatca cggataaatc taccagataa cctattaatt tcatttaatg
    10201 attttgtctt gtctacaagg taattccaat atataacaga tgttttacat acgacgtgac
    10261 catttattaa acatgtgtct ggaagcgtga tggtatattt tataaacttg tcaagatact
    10321 tttgtgagtt aatactataa ccataaatat gatttatcga tgcttttaat tgttctgtgt
    10381 ttgtaactaa aataaaaaag acattattga tgtcaaaaat gtgttttatt gtttcaatga
    10441 catttgttga aaaactcggc ttacatcggt ctaattcatc aattataatt actatctttt
    10501 gactttctga aatgctttcg atgcaagatt taagtgaatt tatgtttttc tctgattcca
    10561 tatggtcttc aagcaaattt tcaatagtcc catctattgc tgcattgctt gctttcttca
    10621 tcgcatcttg gaattcttcg gaaacttcac tagcctcctg tcgtaaaaac caacctgcac
    10681 cagcttttag taccgttttt aaaccaaagc gaattgcagg aagagatctc ttaatgaagt
    10741 gttgtttttc ttcctcaggt aaaatgctgg caattgcaga ggttatgaga agtaatggag
    10801 attctgcatg atccccttta aaggcatcaa tataaacaac tttagattca gtttcttgct
    10861 caataatgag atttttcagt ttgatactaa attctgtttt ccctgttccc catgcgccgt
    10921 ctattaccag tggtgaaatg tctgcctctg gttttagcaa cttgatgata ttttcagcga
    10981 tgtttctgcg ttggaactcg tcacgttcag tgaaagatag tgtatctaac ataatataaa
    11041 cctattaact cctacagtaa aaaacgaata atacgacttt gggattaaaa atcattaacg
    11101 cggtccgcaa aaattcttgt aatctgttaa gagtggttac ttcgccacac agcttaaacc
    11161 cgccgtcgag cgggttttgt cgtttttgtt gctggggatt cgttgggcct ggcctatccc
    11221 gcagttatcc attggttcgg ctctttgacg tttccgcttc tgatttgcgg tacatgatgt
    11281 tccctcaatt tgcacctgct gtatcagcga ggtgagagat aactacaaat gcctcataac
    11341 ccaaatacct ggttggagtt ggtccagagc tggtggcgtg gagacacacc gctgggcgca
    11401 gtgattatgt cgattgttat ggctggcttg cgcattgcat attttggcgg tggtgggggc
    11461 tggaaacgaa aaacgctcga gattttgctc tgtggtgctc tgacgctgac ttttgcatcc
    11521 gctcttgagt atgtcggatg gcctaaatct ctttctgttg ccattggtgg cggcgttggg
    11581 ctaatcgggg tcgatgctat tcgtggggct gcaatgagag taatcggtaa taagtttggt
    11641 ggctctaagg agtaatttat gcaggtacta aattcccagc gtaaagcttt cctcgatatg
    11701 gtggcatggt cagaaggaac ggataacggg cgacaaccga cacgtaatca cggttatgat
    11761 gttattgttg gcggcgaact gtttactgat tactctgatc accctcgcaa acttgtcacg
    11821 ctaaacccca aactcaaatc aacagccgcc ggacgttatc agcttctttc acgctggtgg
    11881 gatgcctacc gtaagcagct tgctctgaaa gacttctccc ccaaaagcca ggatgctgtg
    11941 gcactgcaac agattaaaga gcgtggcgct ttaccgatga ttgatcgcgg tgatattcgt
    12001 caggctatcg accgttgcag caatatctgg gcgtcgttac ccggtgcagg ttatggtcag
    12061 tatgaacata aaatcggtga tttgattgcc cggtttaaag aggctggtgg ggtggtaaat
    12121 gaagctgagc tataagctgc ttatcgttgg tttcttcgtt actgtcattg gttctttcat
    12181 ctggtcggcc aaccactact acagcaaata tcagtacgaa aagaaacgtg ctgatgaggc
    12241 tgtacgaaat gctgaatcag caactgccat taccaataac gtcctgcaat cactacaaat
    12301 cgtcaataca gttctggagg ctaaccagca tgcaaaacag cagatcgcac tggagtcaca
    12361 gagagcccgg gaagatatca aagtggctgt tgcggatgat gattgtgctt cacgccctgt
    12421 gcctgatgtc gctgctgacc ggttgcggaa gtacgcggac agtatacgtg caggttccaa
    12481 cgatgccgtt accgacgaac ctgctcgctg aaactccaca gccagttata cccaatcctc
    12541 tgacttatgg ggatagtctt agtttgaatg taagtctgct atcagcactg gggctatgta
    12601 accgtgataa gtctgatctt cgtaggttag gagagcaaaa gtacaatctg catttgaata
    12661 ataatattca ttaggtgaaa tattattatt tgactgttct agttattatg catttagtta
    12721 caatactctc actattaaca gtgaggtaaa aatgaacgaa aattatattg catatgagac
    12781 acttgtagca aaccgtgctg ctgctgagtg ggcttgttgg gcaatgattg catcttgggt
    12841 gagtgctgga gctactattg ttactttggt tttggcgttc aaggcattgt ttacgtggcg
    12901 ggaacaagag aaaacaaaag taaaaataga ttttaggaat gcattaaaga aattaaagac
    12961 agctctatta tttatgcctg tcaatattga ccccgagaaa ctcaatgatg aacgagagca
    13021 agttattgcg aaatggctat ttaaagatgt agatcttatt agtcagcaaa ttgagttggg
    13081 agaagagaat gttaaaagat ttgatgaact tttgagtatt ttcgattgtt gccagtcttc
    13141 atggtttgcg acagagcact tatttgataa tactgagtta gaaaaagttt ggcatgagtt
    13201 cgagtctaac tttaataaat atataaatgg tggtgagagt aaggatttac ttatgaaaat
    13261 gcttgataag ctcatctctt ctagatttgt atttgagtca agataattgc ctttgagtat
    13321 ttttctttat tattttactt attataaatt ttttatatgc cccctagaat cccaaaagcc
    13381 tgccgtgttc gcggctgccg ctctacaacc acggacccgt caggctactg cgaaagccat
    13441 aaaagcgaag gctggaagca atacaagcca ggacaatctc gtcatcagcg cggttatggt
    13501 tcgaagtggg atgttatccg cgtgcgtgtc ctgaagcgtg acaaaggcct gtgtcagcta
    13561 tgtctgcgtg ctggtgtggt gcgtgaggcg aaaactgttg accacatcat ccctaaagcg
    13621 catggcggca cagatgccga cagcaatctg cagagcctgt gctggccgtg ccataaggag
    13681 aagacggccc gtgaacggct aaagtgatca taattctcaa ctgtctgagg ggaggggcgg
    13741 gtcaaatctc tgtgacctga cgtcttccgg actgcccgcc ccatcgtttt tttatacccg
    13801 cgaaaaatga aatttaacca ggagtgccgc atatggctgg aacggcgggg cgttccgggc
    13861 gtcgccctaa gccaacggcg cgcaaggcgc tggccggaaa ccccggcaag cgagccctga
    13921 acaaagatga acctgttttt acgcccatca aaggtgttga gccaccggag tggttcgctg
    13981 aagaagagct tcctctcgcc acgatcatgt ggcaactgac aaccaaagaa ctctgcggtc
    14041 agggcctgct gtgcgtgact gaccttgcgg tgcttgagcg gtggtgtgtg gcctatgagt
    14101 tctggcggcg tgccgtgaaa aatattgcca gacagggcaa caccatcacc ggtgcaatgg
    14161 gcggtatggt caaaaatccg gagctgaccg ccaaaaaaga acaggagtcc gagatgagca
    14221 gcacgggggc aatgctcgga ctcgacccca gtagccgcca gcgtctgatt ggcctggcgg
    14281 ggcagaagaa agccactaac ccgtttctga aaatcatcga gtcatgagcc ggaaatctta
    14341 ccccaacgta aatgctgcca atcagtatgc ccgtgatgtc gtgcgcggaa agattgtggc
    14401 ctgccagttt gtgattcagg cctgccagcg ccatcttgat gacctgatgg cggaaaaaag
    14461 taagtcgttt cgttaccgct tcgacaagga gctggctgaa cgggccgcga aatttattca
    14521 gctgttgcca cacaccaagg gggagtgggc attcaagagg atgcccatca cgctggagcc
    14581 gtggcagctc tttgtgatct gctgtgcgtt tggctgggtc aataaagggt cccggctgcg
    14641 ccgcttccgg gaggtgtata ccgaaatccc ccgtaagaac ggcaaatcgg caatctctgc
    14701 cggtgttgcc ctgtattgtt ttgcctgtga taacgagttt ggcgcggaag tgtattccgg
    14761 tgccacgaca gagaaacaga cgtgggaagt ctttcgcccg gcgcgactga tgtgtaaacg
    14821 cacacccatg ctgacggaag cgttcgggat tgaggttaac gcctcaaaca tgaaccgtcc
    14881 ggaggatggg gcgcggtttg aaccgctgat tggtaacccc ggtgatggtt catcacccca
    14941 ctgtgcggtg gtggatgaat atcacgagca cgccacagat gcgctttaca ccacgatgct
    15001 taccgggatg ggggcgcgac gtcagccact gatgtgggct atcactaccg ccgggtacaa
    15061 cattgagggg ccgtgctacg acaaacggcg ggaagtcatc gagatgctca acggttcggt
    15121 acccaacgat gaactgttcg ggattatcta taccgttgat gaaggtgacg actggaccga
    15181 cccgcaggtg ctggaaaaag ccaatccaaa tattggcgtg tcggtttatc gcgaattttt
    15241 gttaagtcag cagcagcgtg cgaaaaataa cgcccgtctg gcaaacgtct ttaaaacaaa
    15301 acacctcaat atctgggtgt cggcgcgttc ggcgtatttc aacctggtga gctggcagag
    15361 ctgcgaggat aaatcactga cccttgagca gttcgagggg cagccgtgca ttctggcctt
    15421 tgacctggcg cgtaagctgg atatgaacag catggcgcga ctttataccc gcgagattga
    15481 cggtaaaacg cattactaca gtgtggcccc gcgtttctgg gtaccgtatg acacggtgta
    15541 cagcgtcgag aaaaatgaag atcgacggac agccgaacgc tttcagaaat gggtggaaat
    15601 gggcgttctg accgttaccg atggtgcgga ggtggattat cgctacatcc tcgaggaggc
    15661 caaagcggcg aacaaaatca gtccggtcag tgagtcaccc atcgacccct tcggggcgac
    15721 cgggttgtca catgaccttg ctgatgaaga cctgaacccc atcactatca ttcagaacta
    15781 caccaacatg tccgacccga tgaaagagct ggaagcggca attgaatcgg ggcgctttca
    15841 tcatgatggc aatcccatca tgacctagtg tatcggcaac gtggtcggca aaaccattcc
    15901 gggtaacgat gatgtggtga agcccgtcaa agagcaggcg gaaaacaaaa ttgacggtgc
    15961 agttgcgctg attatggcgg ttggcagagc catgctgtac gagaaagaag acacgctgtc
    16021 tgaccacatt gagtcctatg ggatccgctc gctttaactg aggtaattat gatcatgctg
    16081 attctcgcgc ctctggtggg cgtgctgggg gcgcttttgc tggcgtatgg tgcctggctg
    16141 atttatcccc cggcggggtt tgttgttgcc ggggcgttgt gcctgttctg gtcgtggctg
    16201 gtggcgcgat atctcgaccg tacacagtcg tctgtcggcg gaggtaaata gtgttctttt
    16261 cgggattatt tcaacgaaaa agtgacgcac cggtgaccac gccagcagag ctggcggatg
    16321 atatcgggtt gtcctacgac acctataccg gaaagcagat cagcagccag cgggccatgc
    16381 gactgacggc ggttttttcc tgtgtcaggg tgctggcgga gtcggtcggg atgttgccct
    16441 gcaacctgta tcacctgaac ggcagcctga agcagagagc cactggcgaa cgtctgcata
    16501 agctgatctc cacgcatccc aatggctata tgacgccgca ggagttctgg gagctggtgg
    16561 tcacctgtct gtgcctgcgg ggaaactttt acgcctacaa agtgaaagca tttggcgaag
    16621 tggctgaact gctgcccgtc gatcccagct gtgtggtacc gaagcttaac agtagctggg
    16681 agccggtcta tcaggtcaca ttcccggatg gctccgcgga tgtactgagc caggaggata
    16741 tctggcatgt gcgcacgctg acgctggacg gactggtggg gctgaatccc atcgcctatg
    16801 cccgcgaggc aatatcgctg gcggcagcga ccgaagagca cggggccaga ctgttcagca
    16861 atggcgcggt gacgtcgggt gtgttgcgta cagagcagac gctgtcagat caggcttacg
    16921 agcgcctgaa gaaagatttt gaggagcgtc acaccgggct tggcaatgct caccgcccga
    16981 tgatccttga gatggggctg gactggaagt cgatggcgct gaacgccgag gacagccagt
    17041 tcctggaaac ccgcaagttt cagcttgaag aaatctgtcg tctgttccgg gtgccgttgc
    17101 acatggtgca gaacaccgat cgcgccactt tcaacaatat tgaagagctg gggctgggat
    17161 ttatcaacta ttcactggtg ccgtatctga cccgcatcga acagcggatc aacaccggac
    17221 tggtacgaaa aagtaagcag ggcgtttatt acgccaaatt taacgccggg gcgttactgc
    17281 gcggggatat gaagtcccgt tttgaagcct acgccaccgg gattaactgg ggaatttact
    17341 ctcccaatga ctgccgcgac ctggaagata tgaatccgcg tcccggtggt gatgtctatc
    17401 tcacaccgat gaacatgacc acgaaaccct ccgatggcag taaagccggt aagcagaagg
    17461 ataacgccaa tgcagacgaa acaacgtctt gatgtaccgc tgagtctgaa atctgtcagt
    17521 gactccggtg agtttgaagg gtatggctcc gtctttggtg taaaggacag ccacgatgat
    17581 gtggtgatgt ccggggcatt tgccgcttcc ctgcgggcgt ggagtgacag aaaagcgtta
    17641 cctgcgctgc tctggcagca ccgcatggat gagcccatcg gtgtttacac cgaaatgaag
    17701 gaagacgatg tcgggcttta cgtcaggggg cggttgctca ttgatgatga tcccctggca
    17761 aaacgcgcac atgcacacat gaaggccggt tcgttaaccg gcctttctat tgggtacgtc
    17821 ctgaaggact gggaatacga ccggacgaaa gaagcctttc tgctgaaaga aatcgacctc
    17881 tgggaagtca gtctggtgac gtttccgtct aacgacgagg cgcggatcag cgacgtcaag
    17941 aacgcgctgg cccgcgggga aatccccgaa cagaaaaaaa tcgaaagagt cctgcgtgat
    18001 gtcggactct cccgtaccca agccaaagca ttcatggccg ggggctatat cgcactgtcc
    18061 ctgcgcgacg ctgaggatgt gggctctgca ctgaatgcac tgaaaaatct gaacttctaa
    18121 tcaggagaaa tacgatggcg gtagatatta aagatgtcga acaggtcgcg caggagctgc
    18181 agcagaagtt tgacgacttc aaagcaaaga acgacaagcg cgtggatgcg attgagcagg
    18241 aaaaaggcaa gcttgccggg caggtggaaa ccctgaacgg gaaactcagc gagctggaaa
    18301 atctcaaaag cgatcttgaa aaagagctgc ttgagctgaa acgtccggca ggtggcgcgc
    18361 aaaataaact ggccaccgag cataaagaag cgtttgtggg ctttttgcgt aaaggccgtg
    18421 aagatggtct gcgcgatctg gagcgtaagg cattgcaggt gggcaccgat gaagacggtg
    18481 gctatgccgt gccggaagcg ctggatcgca acattctcac cctgctgaaa gatgaagtgg
    18541 tgatgcgcca ggaagccacg gtgatcaccg ttggcggttc cgactacaaa aaactggtga
    18601 atctgggcgg cacggcttcc ggatgggtgg gggaaacgga tacgcgatcc cagactgcca
    18661 cctccagact ggagctgatt gaacctctca tgggggaaat ctacggcaac ccgcaggcca
    18721 cccagaaaat gctggacgat gccttcttca acgtggaggc ctggatcaac agcgagctgg
    18781 caaccgaatt tgccgaacag gaagaaattg cctttaccac cggcgatggt accaagaagc
    18841 cgaaagggtt cctggcgtat gaatccactg atgaaaccga taaggtccgg gcgttcggca
    18901 aacttcagca tattgtatcc ggcgaagcga cggcggtgac cgcagacgcc attatcaaac
    18961 tgatttacac gctgcgtaag gcacaccgca ccggcgcgaa gttcatgatg aacaacaaca
    19021 gcctgtttgc catccgtctg cttaaagaca gcgagggtaa ctatctgtgg cgtccggggc
    19081 tggaactggg gcagccgtcc tctctggcgg gttacggtat cgctgaaaac gaacagatgc
    19141 cggatatcgc cgctgatgcg aaagccattg catttggtaa cttcaaacgg ggttacacca
    19201 tcgttgaccg tatcggcacc cgcattctgc gtgacccgta caccaataaa ccgtttgtcg
    19261 gtttttatac caccaagcgc accggcggga tgctggtcga ttcgcaggcc atcaaactgc
    19321 tgaagattgc agcggcgtaa tcactcaggg gcgcggaacc gcgccccctg ttctgacggg
    19381 tgaagaatca tgatcctgaa acaagatctg aaatggtcac cggacggtat gcgtgttgag
    19441 gtcattcggg ccggtgagta tgacgacggg gcgcttcctg cccgggtgca ggagattgca
    19501 cttcaggccg ggttagcaga gcgcggaacc agtgcaaaaa gcagtaaagc gacaaaagag
    19561 aaaaaagcca cgaccagtaa agagggctga gtatgcttct gacaatggaa gagattaaag
    19621 cccaactccg gctggatgag gatttcgatg ctgatgaccg ccatctgcaa ctgctggcct
    19681 gtgcggcaca aaagcggacg gaaacgtatc tgaaccggaa gctctatgca ccggatgaaa
    19741 ccattccgga cagcgatccg gacgggctgc acctgccgga tgatattcgt ctggggatgc
    19801 tgatgcttat cagccatttt tacgaaaacc gctcgtcggt tacggaagtg gagaaacttg
    19861 acatgccgca gagttttggc tggcttgtcg gcccgtacag gtactttccg caatgaaaat
    19921 tcgtcaggcg cagaccagcg caacctacat tctgccggac cccggtgaac tgaataaacg
    19981 cgtcctgatc cgcctgcggg tggatatgcc cgcggataac tttggcgtgg agcctcaata
    20041 cctgattacg ttccggacat gggcgaaggt tatccagacc agtgccacca cctggcagga
    20101 aaccgcgcag accggggacg ccatcaccca ttacatcacc attcgttacc gccgggggat
    20161 taccgctgat tatgaggtgg tctgcggtga cagtgtgtac cgggtgaaac gtcagcgcga
    20221 tctgaacggg gcgcggcgct ttctgctgct ggagtgtacg gaactgggcg aatgtgggca
    20281 gagtcacgga ggcaacaatg acgacttcct ttttgcacgt tgattttcag cagcccgcgg
    20341 agatgcgctt taaccgcgcc cgtgtccggc gggcgtttgt cacgattggt cagcgtcata
    20401 tgcgtgatgc ccgtcggctg gtgatgcgcc gtgcgcggtc ggcaccgggt gaaaaccccg
    20461 gttatcagac cggacgcctg gctcgttcga ttggttacat ggtacccaga gccagtaaac
    20521 atcgccctgg ttttatggca cgtatagccc ctaaccagcg taatggagag ggaaaccgcc
    20581 gtatcaccgg tgatttttat ccggcttttt tgttctatgg cgtgaggcga ggggcaaagc
    20641 gtcgtcgcag ccatcatcgt ggtgcatccg gtggcagcgg ctggcgactg gctccacgta
    20701 ataactttat ggtggaaacg cttgaaaaga accgcagctg gacacgctat tttctggcgc
    20761 gggaattgcg taaatcactg aagccggagc gacgacacag atgaaactga cgcctgttat
    20821 tgctgcgctg cgtgcccgct gcccgtattt tgaaaaccgg gtggcaggcg cggcacagtt
    20881 caaaaatctg ccggaggtcg gaaagctgag actcccggcg gcgtatgtgg taccgggtga
    20941 tgactctccg ggagaaaaca aaagccagac cgactactgg caggagctga aagagggctt
    21001 ctccgtggtt gtcatactga gtaacgggcg tgatgagcgc ggtcagtttg cctcgtatga
    21061 tgtggtggac gatgtccggc aaatgctctt taaggccctg ctgggctgga acccggaagc
    21121 gtgcggtaac ccgattacct atgacggcgg cacgctgctg gatctgaatc gtcatgagct
    21181 gatttatcag ttcgattttt cggtcatcag cgagttgacc gaagacgata cccgccagca
    21241 ggatgacctg aacagtctgg atgaactgcg aacgctggcg attgatgttg attatctaga
    21301 gcccggtaac gggcctgacg gcgatatcga acatcacacc gaaataaccc ttccttcctg
    21361 agaatcttca tgtttgtgaa acctgttaaa gggcggtcag ttcctgaccc tgcccgcgga
    21421 gaccttttgc ccgccgaggg gcgaaatgtt gatgagaaca actactggct gcgccgtgaa
    21481 gcagcgggtg atatccggcg cgtgaatgaa aaggtgaata ccgatgacga gctttaacac
    21541 cattccgtca aatacgctgg ttccgatttt ttatgcggaa atggataact cggcggcgaa
    21601 tactgcacag gacagcggag catcgctgct gattggtcat gccaataacg gtgcagagat
    21661 tgttgccaac agtctggtgc tgatgccatc ggcagactat gcacgccaga tttgtggtgc
    21721 gggaagtcag ctggcgcgta tggtcgaggc ttatcgccag accgacccgt ttggtgagct
    21781 gtatgtgatt gccgttccag aagccacagg cgcggcggca acggttacgc tgacggtgac
    21841 cggggaagca accgaaagcg gcacggtgaa tgtctatgtg ggacgtaccc gcgtgcaggc
    21901 tccggtgacc aacggcgata acgtcacgac gattgccagc agtatccagg atgccatcaa
    21961 tgccgttccg gccctgccgt ttacggcttc atcttcggca ggcgtggtca cactgaccgc
    22021 gcgtcataag gggctttgcg ggaatgaaat tcctgtcagc ctcaattact acggctttgg
    22081 tgggggcgaa gtgctgccag cgggcgtaca gattgccgtg gcgacgggta ccgccggaac
    22141 gggtgctccg gttctcaccg gcgcggtggc tgcaatggcg gatgagccgt ttgattatat
    22201 cggcctgccg ttcaacgaca cggcctccgt taacacgctg gtgaccgaga tgaacgatac
    22261 cagcggtcgc tggagctatg cgcgtcagct gtatggtcat gtgtatacgg caaagatcgg
    22321 cacgctgtca gaactggtga ccgcaggtga ccagtttaac cagcagcaca ttaccctggc
    22381 gggatacgaa aaagagaccc agacgcctgc cgacgagctg gcggcaagcc gtaccgcccg
    22441 cgcagcggtg tttattcgca acgatccggc acgtcccacg cagaccggtg agctggtggg
    22501 tatgctgcct gcgccgaagg ggaaacggtt cacgatgacc gaacaacaga ccctgctgtc
    22561 tcatggcgtg gcaacggcgt atgtcgaaag cggggtgctg cgcattcagc gtgatgtcac
    22621 cacgtacagg aaaaacgctt acggtgttgc ggataacagc tacctcgaca gcgagacgct
    22681 gcataccagc gcgtatgtgc tgcgcaaact gaaatccgtc attaccagta agtacgggcg
    22741 tcacaagctt gccagcgacg gtacccgctt tggtcccggt caggcgattg tcacaccggc
    22801 ggtgatcaaa ggggaactgc tggcaaccta ccgtcagctt gagcgtgcgg ggatcgtgga
    22861 aaactacgaa ctgtttaagc agtacctggt tgtggagcgt gatgccagcg atccgaaccg
    22921 cctgaacacg ctgttcccgc ctgactatgt taaccagttg cgtgtctttg ccgtggttaa
    22981 ccagttccgt cttcagtatt cagaggagtc tgcataatgg cccgtatcgg gggaacctgt
    23041 tatttcaaaa ttgacggtca acagctatcg ctgaccggcg gcattgaggt gcccatgaac
    23101 aggacggtca atgatgacat catcggcctg gacggttcag tggaccgcaa ggaaactcac
    23161 cgtgcgcctt atgtcaaagg gaccttcaag gtgccgaaga attttccggc gagcaaaatc
    23221 acctcgtctg atgagatgac catcactgcc gagctggcga acggtcaggt ctatgtactg
    23281 tcgtctgcct ggctgcacgg cgaagcgaac cataatgccg aagaaggcac ggtcgatctt
    23341 gagttccacg gtgaagaagg ggattaccag taatgaaaga gcttgagtta aagaaaccga
    23401 ttaccgctca tggcgagaca ctctccgtac tggagtttga tgagcccacc gggaaagatg
    23461 tccgcgagct ggggtatccc taccagatga atcaggatga gtccgtcaga cttctggcgc
    23521 atgtggtatc gaaatatatt gtgcggctgg cgaaagtgcc gcaaagctct gtcgaccaga
    23581 tgtctccggc agacctgaat gcagcggcgt ggcttgtggc tggttttttc ctccaggcct
    23641 gacggctgaa taccttactg atcgcttctt tgattgcgcc agctactggc gcattaatcc
    23701 cttcgaattg ctgaatatgc cgatcagtga aattcccttg ctggtcagtc aggcaaacag
    23761 gatagagcag gagaaacgca cacatggctg aatttgagct taaggcgttg atcaccggtg
    23821 tcgacaggct ttctcccgcg ctgtcgaaaa tgcaaaagaa aatccgggga tttaaacgcc
    23881 aggcggaaga agcgtcacag ggtgggctgg cgcttggtgg cggactggca gcgggtctga
    23941 cgctttccct gaaatcttat gccgatcagg aaaacgccgc caccgggctg aaagtcgcca
    24001 tgatggatgc gaatggcgag gttggaaaga gctttcagga catcaataaa ctggctattg
    24061 gcctgggtaa ccagctaccc ggtacaacgg ctgatttcca gaacatgatg cagatgctgg
    24121 tgcgtcaggg gatcccggca gaaaatattc ttggcggtgt gggtaaagcg acagcttatc
    24181 ttgcggtaca actgaaaaaa acaccggaag cggctgctga gtttgctgca aagatgcagg
    24241 atgctaccgg aacggcgtca gaagacatga tggggctgtt cgacactatc cagaaggcgt
    24301 tttatctggg cgttgacgat accaacatgt tgtccttctt cactaaaacc agttctgttc
    24361 tgaagatggt gaacaaggac ggtcttcagg ctgcacagag ccttgccccc atcagcgtca
    24421 tgatggatca gatggggatg aacggggagt cggcaggtaa tgccctgcga aaagttatcc
    24481 agtccggatt aagcgttaag aaaatcaggg acgtcaataa aatcatggcc cgccagaaac
    24541 tcggggtaca gctcgatttt actgacggca aaggaagttt tggcggtctt gataacatgt
    24601 tcaggcaact ggcaaagctg cgaaaactga ccgacgttaa gcgaacaggt gtacttaagg
    24661 caatatttgg tgatgatgcc gaaacccttc aggtggtcaa tgcactaatc gataaaggaa
    24721 aggatggcta cgatcagatc cagcagaaga tgaataaaca ggccagcctg aataaacgtg
    24781 ttcaggccca gcttggtacg ctgtccaacc tgtgggaggc aatgacgggg accgcaacta
    24841 acggccttgc ggctattggc ggcgcatttt ctggtgacgc caaaaatatc acgcaatggc
    24901 tgggggagtt gggggagaaa ttcacgaagt ttgcggatga aaatccccgg gttattcgcg
    24961 gcgtcgtcgg gcttgctgcc ggtcttgcga ttctgaaact gggattgatg ggcgtgggca
    25021 gtgccatcag tatcgtcagc aggatcatgt cgatgacgcc gattggcatg attgcgacgg
    25081 cgattgccct ggctgcggga ttaattatca ctaactggga tgttgtcgga ccttatttta
    25141 agaaactctg ggaaaccatt agtccttatt ttgaggctgg ctgggaactt ctgaagaagg
    25201 tttttgcctg gtcgccgctg gggatggtga tcaataactg gggaccggtt gttaagtggt
    25261 ttcaggatat gtgggataag ctgaagccaa ttattgagtg gtttaccgac agttcaggtg
    25321 acacggtcga tgccattaac tcggcgcagt ggggcgcggg tgcttatgat gcttatggga
    25381 cgggaatacc ggcgcgggga tacatacctt atccggcggt ggatctggct cagtcaaaca
    25441 acgcctccga tgccacaggc ccgaatccct tcatgattaa caaagcttct gcgccaaaag
    25501 ttgatggtga gatcaaggtc tcttttgtga attcgcctcc gggtatgcgg gttatggaaa
    25561 cgcgatccag cggttttgat gtcagccatg atgttggcta tacgcgcttt ggcaggtaat
    25621 gaaaaattaa tctgttaatg agtcccactc cggtgggatt ttttatgtac ggagtttata
    25681 tgacgtggaa agacagactt caggacgcgt catttcgcgg tgtgccgttt aaggttgaag
    25741 aagaaaatgc gggaaccggt cgtcgtgtgg aaacgcacga atacccgaac cgcgacaaac
    25801 cctataccga agatctggga aaagtcactt tccgcccgtc catcacggct tatgtggtgg
    25861 gagatgactg ctttgaccag cgcgatcgcc tgattgacgc gctgaataaa cccggtcccg
    25921 gcacgcttgt ccatccgaca tacggtgagc tgaaagtctg tgttgacggg gaggttcggg
    25981 tcagcacatc gaagggtgaa gggcgtattg tccgctttga cctgaagttt gtcgaagcgg
    26041 gagaactctc ttaccccaca tcaggtgcgg cgacggcgca gacgctgatg tcatcctgtt
    26101 ctgcactgga tgactgcatc agtgacagtt tcagtggttt cagtatcgat ggcgtggcag
    26161 attttgtgca gaacgacgtc gtcggtaatg ccagcacaat gcttgggtat gtttctgatg
    26221 cgatgaaagt ggtggattct gccgtatcgg atgtcgccag gctgttgcag ggggatatct
    26281 cggtacttct gccgccgcca tcgtcaggca aaaatttcgt tgagcaggtg cagaaaatgt
    26341 ggcgtaccgg gaaacgcctt tatggtaacg ccagcgacct ggtcaccatg atcaaaacgc
    26401 tttccggtgt cagcctcggc agcgatctgc aaccgcgcgg cgtctggaaa acggacagta
    26461 aaaccaccgc cacggcgacg cagcagcgta acgtggttgc cagcaccctt cgtacgaccg
    26521 caatcagcga agcggcgtat gccgtcaccc gattgcctgc gccaacaact tccgcggtga
    26581 tgcagaatgc cgcagtggag caggcaacaa cacctgcgca gagcactggc tggccttccg
    26641 tcacgcatcc ggcactgaac aatgcaccgg cggtgaaaaa cacggttgac ctgccgacgt
    26701 gggaagaact gactgacatt cgcgacacac tgaatacggc aattgataag gagttgtccc
    26761 gtacaaccag tgatgcgctg tttctggcgc tgcgccgggt gaaagcagat ctgaatgcgg
    26821 atatcaacac gcgccttgaa cagtctgcac ggatcattca gcgcacaccg gatgaggttt
    26881 tacccgcgct ggtgctggcg gcgacctggt ttgataacgc ggcgcgtgac gcggacatta
    26941 tccggcgtaa tgccattacg catcccggct ttgtgccggt gatccctctg aaggtgccag
    27001 tgcaatgaac gataacgtca cgctacgggt aaatggccgg gagtggaatg gctggacatc
    27061 ggtgcgcatc ggtgccggta ttgaacggct ggcgcgggat ttcagtgtgg agatctcccg
    27121 ccagtggccg ggagatgggg gtatcaccac gcttcagccg cgcattaaaa acggttcaaa
    27181 agtggaagtg ctgattggtg atgagctggt gatcaccggc tgggtggagg cgacgcccgt
    27241 tcgttacgat gcccgttcgg tcagcaccgg tattgccgga cgcagtctga cggctgacct
    27301 gattgactgt gcagccgaac cgacacagtt taacggacga gagctggtac agattgcgca
    27361 ggcgcttgct gcgcctttcg gcattgaggt ggtgaacaac ggtgcgccgt cgggtgttat
    27421 tcctgacgtc cagcccgatc acggtgaaac ggtgattgag gtaatcaaca aaatactcgg
    27481 tcagcagcag gcactggctt acgacgaccc gcacggcagg ctggtgattg gtggtattgg
    27541 ctcaacgcgg gcacatactg cgctggtact cggggaaaac atcctttcct gcgatacgga
    27601 gaagagtatc cgggagcggt tttctgttta ccaggtggcg gggcagcgtg ccggaaacga
    27661 cgatgatttc ggtgaggcca ccaccaccgc gctgcgggcc cgcacagagg acgcatttat
    27721 tgcccgttac cgtccgatgt atatcaggca gacagggcag gccacggggg caggctgtat
    27781 tgcgcgtgct gactttgaag cccggcaacg ggcggcgcgg acggatgaaa ccacctatgt
    27841 ggtgcagggc tggcgacagg gtaacggtac gctgtggcag cccaaccagc gggtgattgt
    27901 cttcgatccg gtctgtggtt ttgacaatac cgaactgctt gtctcggaag tcacgtttac
    27961 tcaggaccag aatggcactc tgacggaaat ccgtgtcggc ccgcctgatg cttatctgcc
    28021 tgaacccgaa gaccccggcg cgcggaaaaa gaaaaaagcc agagtacagg aggacccgtt
    28081 ctgatgagga cgattgaagc catgcagcga caactcctcg gcctgattgg gcgggccgtg
    28141 gtgaaaagca tcagtgccgc cacgaaatgt ctgaccgtgg atgtgtccct gattgccggt
    28201 gaacccaaag ccggggttga acatcttgaa ccctacggtt ttaccgcaag ggcaaacagc
    28261 ggtgcggaag cggtggtgtt gtttccggat ggcgaccgtt ctcatgcggt ggttgttacg
    28321 gtgtcagacc ggcgctaccg cctgaaaggg ctgcagacgg gtgaggtggc tgtctatgac
    28381 gatcaggggc agtccgtgac gctgacccgg gaggggatcg tggtggacgg tgcaggtaaa
    28441 acgatcacgt ttcgcaatgc acctgaagca cgttttgaaa tggacctgga agtaacagga
    28501 caggtgaaag acctgtgcga ctccggcggc accaccatgt cagcaatgcg gcttgcctat
    28561 aacggtcatc gtcacagaga gaacggtcag ggcagtaaca ccgataaacc tgataaagct
    28621 atggaggcat gatggaactg tggctgacgg tgaacggtaa acgcacctgc gccagcgcac
    28681 cgctggatcc gctgacccgc gccgtggtga tttccctgtt tacctggcgg cgggcggagc
    28741 ctgatgacaa cgccgacgtc ccgatgggat ggtgggggga tacctggcct gcggtacaga
    28801 atgaccgtta cgcctccaga ctgtggctgc ttcagcgcag caaactgacc aatcagctgg
    28861 tgcagacggt aagggggtat atccgcgaat gcctgcaatg gatgactgat gacggcgtgg
    28921 tgtcccgtat tgatctggat atccgccgca ccgggattaa tgaactgggt aacagtatca
    28981 ctctctggcg tcgtgacgga ccggtaatga tttcttttga tgatctgtgg agtgcgataa
    29041 cgcatggcgg acagtgaatt tcagcgcccg acgctggcag aaaatatcag tatgctccgt
    29101 aacgatttat tcgccaggct ggacgtcagc gacacgctcc ggcgcatgga tgaagacgtg
    29161 cgggcaaagg tgtatgcggc ggcgctgcat acggtttacg ggtacatcga ttatctggca
    29221 atgaacatgc tgcctgacct gtgcgatgag ttctggctgg cgcgacatgc tgcgatgaaa
    29281 cggtgtccgc gcaagggggc cacggctgcc agcgggtata tgcgctggga aggtgtcagc
    29341 gatggcctga aggtgaccgc cggaagtgtt attcagcgcg atgacctggt tcagtacacg
    29401 gcaactgccg atgcaaccag ctccggtggt gtcctgcgcc tgccgatcgc ctgttcaact
    29461 gcaggcgcgg tcggtaacgc tgacgacggt acggcattaa tcctggtcac gccggtgaat
    29521 ggtctgccgt cttccggtgt ggcagatacc ctgacaggtg gatttgatac tgaagatctg
    29581 gaaacgtggc gcgcccgcgt cattgagcgg tattactgga cgccgcaggg cggggctgac
    29641 ggggactatg tcgtctgggc taaagaagtg cccggcatta cccgcgcatg ggcataccgt
    29701 cactggatgg gaacggggac tgtcggtgtg atgattgcca gtagtgacct gattaatccc
    29761 attccggaag aatcaacgga aacggcggca agacaacata tcgggccgct ggccccggtg
    29821 gcaggctctg atttgtatgt gttcaggccg gtggcgcata cggtggattt tcatatccgt
    29881 gtgacgccgg atacaccgga aatacgggct gccattaccg cggagttgcg ttcgttcctg
    29941 ctgcgtgatg gttatccgca gggagaactg aaggtatcgc gtatcagtga ggcgatttcc
    30001 ggtgcggacg gggaatacag ccatcagttg cttgcaccgg tggacaatat ctccattgcg
    30061 aaaaacgaac tggcggtact ggggacgatt tcatggacgt gacaaacgat gattacatcc
    30121 gcctgttatc ggcactgttg ccgcccggtc cggcgtggtc atctagcgat ccggcgattg
    30181 ccggtgcggc accgtcatta acccgtgttc atcagcgtgc ggatgccctg atgcgggagc
    30241 tggatccgcg caccaccact gaactgataa accgctggga gcgtctgtgc ggtctgccgg
    30301 atgaatgtat tcccgcagga atacagaccc ttcgccagcg tcagcaacga ctggatgcga
    30361 aggttaacct ggcgggcggc atcaatgagg atttttacct tgcacagctt gctgccctgg
    30421 gcagacaaga cgccaccatc acgcgatacg acaaaagcac gttcacctgc tcatcggcct
    30481 gtactgacgc ggtgaatgcg ccggaatggc ggtattactg gcaggtcaac atgccagccg
    30541 ccaccaacag cacctggatg acatgtggcg atccctgtga ttccgcactg cgtatctggg
    30601 gcgacaccgt cgttgaatgt gtgcttaaca aactctgccc ttcgcatacc tacgtaattt
    30661 ttaaatatcc ggagtaatcc atgcatcgta tagacacgaa aaccgcgcag aaggataagt
    30721 tcggcgcggg taagaacggt tttacccgtg gtaaccccca gaccggcaca cctgccaccg
    30781 atctggatga tgactacttt gacatgttgc aggaggagct ttgcagcgtg gtggaggcat
    30841 ccggtgccag cctggagaag gggcggcacg atcagttgct taccgcactt cgcgcgctgc
    30901 tgttaagccg caagaatccg tttggcgata tcaaatcgga tggcacggtg aaaacggctc
    30961 tcgaaaacct tggtttggga gaagcagcta aaaggaatgt aggtacaggg gcgaatcaga
    31021 tacctgatat gagcctgttc gcgtcaatta ataccgtaac ggctgctgcg caaaaatttc
    31081 cgtctggatt aattttacag tgcggtcagt tgaatggtgc cccgaatgta tcttcaacat
    31141 acgggatgag gttcccgatg acgttctcaa gagtcattgc tgtcgtagtt acattgaacg
    31201 ttactggcgc ggcagggcag ccgactgtat cggcgacaag tgtccagaac actggattta
    31261 atattacggt gtcgcccggt tcaggatacg gttcatctgc tgatgcgtat tacattgcaa
    31321 tgggatatta acaaaatgtc atatttttat tctgcatcga caaacggatt ttatttgact
    31381 gaatttcacg gcaccaatat tcctgatgat gcagtggaaa tctcggaatc agagtggaaa
    31441 acactgatta atgcacagag tgtaacaaaa atgattacct gtggtgagaa cggtcatcct
    31501 gtcattgttg accgtccttc tccaacacca gaacaattag ccttaataaa tgatgaaaag
    31561 aaatctgcac tgatagcaga ggcaacgaat gtaatagctc cgcttcagga tgcggttgat
    31621 ttaggtatgg caacagatga tgaaacgaaa ctgttactgg catggaaaaa atatcgggtg
    31681 ctgcttatgc gtgttaatgt agtaaaaccc gagtggccta tgcatccaaa taaatagact
    31741 tgttaatcta ggataattgt tggaataata ctcatttttc agtaggttca ggtagcctgg
    31801 gtataatgct cacttctaca atgatttcaa cttattttat ttaattgata ttacgaaact
    31861 taactcatac ataaacaata tgactcaata ctgtcctaaa tagctgcgca gaaaagtgct
    31921 ccactttcgc tcagcccttg ctgggcgaag catcaggaag gcgttctcag ctaggttgtg
    31981 ttatatctgg cttgagtttt gttgcccata cgcaacacat tacccatctg taaacgacaa
    32041 gtgtgacaga attaggtgca gctatttaga ctatatcgac gcagtaacga taatagatat
    32101 cgaaactgta ctagatatta gctacttatg gttaccgggg gaagtctttt gagcactggt
    32161 gttatcaatc ggcttcaaat aattcaccaa taaattatat tgcgatagct catcaccttc
    32221 ctggttgttt atagccagtt tatcagcgtc accacctact gtttatgctt ttatttcttt
    32281 gattaatgag tatatgatat tttaattcat ctggtgtatt tctttctcat gtgttggccg
    32341 actgaatatc tgttcagtga aggggtaatt ttatgtttgc acttcgaaca ttcccttcag
    32401 ttgccaagaa agaaaaccgt aacatttgcg cattaatttt aacagtataa tctgagcata
    32461 aatatgcgct atatatctat aaaaaggcgc aattaattgc gcctttttat cattttatca
    32521 aaaatggccc accgactgtc ctcgatagcc aatggccaat tttatctgat acatatacat
    32581 aaatacaggt atatatgatt gcgcagatag gatttggaat ggtcacgctg aagcctatga
    32641 ttgctaaagc catagggata aataatttgg tgatatactc cattccacat atgttaagag
    32701 tatttcttcc agctcttaca ataaagtcat tcccacaaat tatttttgct ataaaaatat
    32761 taaatattat caaacctacc gtggtgataa tagtattaat agcttgaata taactacttg
    32821 atattatgtc tgcattgaat attgagataa tcttaaaaag caaaggtttc tggttaagga
    32881 gttgatacgc actcattatc gcggtaattg agaagacaat gaaacca
//
LOCUS       DADMGC010000032        32719 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.032, whole genome shotgun sequence.
ACCESSION   DADMGC010000032 DADMGC010000000
VERSION     DADMGC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.032"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..869)
                     /locus_tag="JNZ73_001486"
     CDS             complement(<1..869)
                     /locus_tag="JNZ73_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY8731810.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNREGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLP"
     gene            complement(1047..1958)
                     /gene="gsiD"
                     /locus_tag="JNZ73_001487"
     CDS             complement(1047..1958)
                     /gene="gsiD"
                     /locus_tag="JNZ73_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HAY8731811.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1961..2881)
                     /gene="gsiC"
                     /locus_tag="JNZ73_001488"
     CDS             complement(1961..2881)
                     /gene="gsiC"
                     /locus_tag="JNZ73_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HAY8731812.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2899..4437)
                     /gene="gsiB"
                     /locus_tag="JNZ73_001489"
     CDS             complement(2899..4437)
                     /gene="gsiB"
                     /locus_tag="JNZ73_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HAY8731813.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDSGFSFED
                     ADLQ"
     gene            complement(4457..6328)
                     /gene="gsiA"
                     /locus_tag="JNZ73_001490"
     CDS             complement(4457..6328)
                     /gene="gsiA"
                     /locus_tag="JNZ73_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HAY8731814.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6315..7280)
                     /gene="iaaA"
                     /locus_tag="JNZ73_001491"
     CDS             complement(6315..7280)
                     /gene="iaaA"
                     /locus_tag="JNZ73_001491"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY8731815.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7484..8719
                     /gene="moeA"
                     /locus_tag="JNZ73_001492"
     CDS             7484..8719
                     /gene="moeA"
                     /locus_tag="JNZ73_001492"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAY8731816.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPISATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8719..9468
                     /gene="moeB"
                     /locus_tag="JNZ73_001493"
     CDS             8719..9468
                     /gene="moeB"
                     /locus_tag="JNZ73_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HAY8731817.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9647..10309)
                     /gene="fsa"
                     /locus_tag="JNZ73_001494"
     CDS             complement(9647..10309)
                     /gene="fsa"
                     /locus_tag="JNZ73_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY8731818.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10440..11339
                     /locus_tag="JNZ73_001495"
     CDS             10440..11339
                     /locus_tag="JNZ73_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAY8731819.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11345..13777
                     /locus_tag="JNZ73_001496"
     CDS             11345..13777
                     /locus_tag="JNZ73_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HAY8731820.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHYELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMAAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13923..14738
                     /gene="ybiV"
                     /locus_tag="JNZ73_001497"
     CDS             13923..14738
                     /gene="ybiV"
                     /locus_tag="JNZ73_001497"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HAY8731821.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14890..16155
                     /locus_tag="JNZ73_001498"
     CDS             14890..16155
                     /locus_tag="JNZ73_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HAY8731822.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(16396..17988)
                     /locus_tag="JNZ73_001499"
     CDS             complement(16396..17988)
                     /locus_tag="JNZ73_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HAY8731823.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEGFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPECVIDFSGNYEDYLRSKGIE"
     gene            18207..19127
                     /gene="ldtB"
                     /locus_tag="JNZ73_001500"
     CDS             18207..19127
                     /gene="ldtB"
                     /locus_tag="JNZ73_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY8731824.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(19185..20303)
                     /locus_tag="JNZ73_001501"
     CDS             complement(19185..20303)
                     /locus_tag="JNZ73_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HAY8731825.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGMQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(20300..20767)
                     /locus_tag="JNZ73_001502"
     CDS             complement(20300..20767)
                     /locus_tag="JNZ73_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HAY8731826.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20953..21081
                     /gene="mntS"
                     /locus_tag="JNZ73_001503"
     CDS             20953..21081
                     /gene="mntS"
                     /locus_tag="JNZ73_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HAY8731827.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            21353..22936
                     /locus_tag="JNZ73_001504"
     CDS             21353..22936
                     /locus_tag="JNZ73_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY8731828.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22984..23499)
                     /gene="ompX"
                     /locus_tag="JNZ73_001505"
     CDS             complement(22984..23499)
                     /gene="ompX"
                     /locus_tag="JNZ73_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HAY8731829.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23552..23617)
                     /locus_tag="JNZ73_001506"
     CDS             complement(23552..23617)
                     /locus_tag="JNZ73_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731830.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23852..24739
                     /gene="rhtA"
                     /locus_tag="JNZ73_001507"
     CDS             23852..24739
                     /gene="rhtA"
                     /locus_tag="JNZ73_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HAY8731831.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            25038..25541
                     /gene="dps"
                     /locus_tag="JNZ73_001508"
                     /gene_synonym="pexB"
     CDS             25038..25541
                     /gene="dps"
                     /locus_tag="JNZ73_001508"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HAY8731832.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25945..26691
                     /gene="glnH"
                     /locus_tag="JNZ73_001509"
     CDS             25945..26691
                     /gene="glnH"
                     /locus_tag="JNZ73_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HAY8731833.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEVYKKWFSTEPK"
     gene            26830..27489
                     /gene="glnP"
                     /locus_tag="JNZ73_001510"
     CDS             26830..27489
                     /gene="glnP"
                     /locus_tag="JNZ73_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HAY8731834.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            27486..28208
                     /gene="glnQ"
                     /locus_tag="JNZ73_001511"
     CDS             27486..28208
                     /gene="glnQ"
                     /locus_tag="JNZ73_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HAY8731835.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            28630..30693
                     /gene="ybiO"
                     /locus_tag="JNZ73_001512"
     CDS             28630..30693
                     /gene="ybiO"
                     /locus_tag="JNZ73_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HAY8731836.1"
                     /translation="MLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQK
                     VTEVSRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIR
                     LCALPLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNA
                     GSRTIAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLS
                     SLIGYGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAE
                     HSLAFFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAA
                     FVSGMFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAW
                     GLFDFWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSA
                     RTRTLLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDI
                     ITGVFIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANF
                     VRGIGSVVANYDVDRHEDADKANQALKDAVTELMENEEIRGLIIGEPNFAGIVGLSNT
                     AFTLRVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPP
                     GEPTL"
     gene            complement(30690..31616)
                     /gene="rlmF"
                     /locus_tag="JNZ73_001513"
     CDS             complement(30690..31616)
                     /gene="rlmF"
                     /locus_tag="JNZ73_001513"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HAY8731837.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            31892..32152
                     /locus_tag="JNZ73_001514"
     CDS             31892..32152
                     /locus_tag="JNZ73_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY8731838.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            32417..>32719
                     /locus_tag="JNZ73_001515"
     CDS             32417..>32719
                     /locus_tag="JNZ73_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HAY8731839.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGV"
BASE COUNT         8022 a         8735 c         8098 g         7864 t
ORIGIN      
        1 ggtagataaa aaagtttatc tgatgcgttc aataaattac cgccaggata ataaaaatag
       61 atttcgaaca atgcattatt tggcactgct gatgtctttg caatatactc cgtatcgaca
      121 gaatcaataa tattataatt accttctcta ttataaataa tcagtttttc acgagcgagt
      181 tgattcttgg caatgatctc atgaccttgt ttcaaaaata gaacttcatc attagcgctg
      241 atttgatcaa gatactcgtt cagataccgc cagtttaaat cgacatgaat tttatctacc
      301 gctaacaaat cgtgatcttt gccgttgata cgtagcgtaa aagagataca ttttttaccc
      361 ggacgatcaa agcaattgta tgccggattc cagctgttgt tagagtcatc tgaagccacc
      421 gaccacggtc gtttcgatgg gttaaaatcg gcaaaaataa cccgccctga ctcgtcgaca
      481 agcggtccat ttacttgttc ctgatgataa acaacgattt tatcattcgc cctccgcgaa
      541 aagagataat atttattatc atcaagtacc agtcccaccg agtctaaaaa tggcatcatt
      601 tccattttca gaatcacggc gcgttgaatc tcctctgtat tttttaataa atacagtgag
      661 tcctttaatg tcagggccga acgttcaata tctttaaaga aatagccaac gtttttctga
      721 ataagttcaa agcggctatc ggcacgtttt accgcgcgtt ccgataccag ttgatagtta
      781 atcatgaaaa aaagaccaat aaatgacagt gctgccaata aaaataaaat tatcagcctt
      841 atctttatct tttcgtataa actcagcatg ttaatacatt taacaaaata actatctgaa
      901 cgctgtgccg ctgcaaaaat agtcccttgc tgcggctttt taaccaaatt acccgattct
      961 acaaaatcag cagaaagtta caacacttgc ttagggcaat aatttcctgt gaggtgatta
     1021 ccctttcaag caatattcaa acgtaactat cctttaattt tcggatccag cgcatcgcgt
     1081 aaaccatcgc ccaacaaatt gaacgccagt acggtcagaa aaatagccag ggccggaaaa
     1141 acagcgacat gcggcgcgat aaccatatcc gctcgtgcct cattgagcat tgctccccac
     1201 tctggtgtcg gcggctgcgc acctaggccg agaaatgaca ggctggcggc agagataatc
     1261 gaggtgccaa tgcgcatggt gaaaaacacc acgatagaag agacggtccc aggcaggata
     1321 tgacgcaata aaatggtcat atcgctggca ccaatactgc gtgctgactc aataaaggtt
     1381 tgctgtttca acaccagcgt gttgccgcgc accaggcggg caaacgcggg gatggaaaaa
     1441 atggcgactg cgataatcac gttagcaatg ccgcttccca acaccgcaac aacagcgatc
     1501 gccagtaaaa tacccgggaa ggcaaacagc acatcgcaaa tgcgcatgat cagccgatcc
     1561 caccagcctt cataatatcc agccaacaag cccagcaacg tccctatcgc cacaccgata
     1621 aacacggcaa acacgcccgc cgccagcgag atttgcgcac caaccaggac acggctgaaa
     1681 atgtcgcgcc ccagtgaatc gacgccaaac cagtgctgca aagaaggtcc gttattcaga
     1741 ttgtcataat caaaataatt ttcggcgtca tagggagcga tccagcgtgc aaaaatggcc
     1801 accacaatca ataaaataac gaataatgcg gcggtcatcg ccatatgctg acggcgaaat
     1861 cgtcgccaga attcatgcca cggtgtacgt acctggtcag gtttgaccag tggcatggcg
     1921 tttaacaccg cctgacgtcg ccagttaaat agtcgcatcc ttacttgtac ctgatagccg
     1981 ggttaatggc ggcgtaaagc acatccacca ctaagttgat aagaataaat tccagcgaga
     2041 aaagcagaat ttccgcctga atcaccgggt aatcgcgcat ttctacagag tcaacgagta
     2101 agcgtccaag tccgggccag ttgaaaactt tctccaccac gatagaacct ccgagcaaaa
     2161 agccaaactg taagcccatc atggtcacta ccgggatcat cgcgttgcgt agcccgtgtt
     2221 tgaggacaac ccaggtttcg ctcacccctt tcgccctcgc ggtacgcata taatcttcgc
     2281 ttaaaacgtc gacaaatgac gcacgggtaa agcgcgccat cacggcggcc actgccgcgc
     2341 cgagcgtcag ggagggtaaa atgtagtgct gccaactgtc tgctcccaca gtaggcagcc
     2401 agcccagttc aacggagaat acctgaatta aaaacatccc cagcgcaaat gccggaaacg
     2461 agatccccga caccgcaatg gtcatactca atcgatccgg ccaacgatta cgccagacgg
     2521 cggcgataat tcccgccgcc ataccaaata tcaccgccca gaccatactg gttatggtca
     2581 gccacagcgt tggcataaag cggctggcaa tctcatcggc aaccggacga cgcgacacca
     2641 tcgacaggcc aaaatccccc tgcacagcat tgctgatata gtgccagaac tggtgataca
     2701 gcggctgatc caaccccagc tgctgacgca ccagttctat aacctgcgca tcagcttcgg
     2761 gcccggcaat caatcgcgcc ggatcgccgg gcagcatgtg gacaaataaa aacaccagca
     2821 ccgagacgat aaacagcgtc ggaatcaacc ccagtaatcg tttgataacg taattaagca
     2881 ttccactccc tgcgttgctt attgcaaatc cgcgtcttca aagctgaagc cgctgtctgg
     2941 catgatccaa aaaccggtca ggtttttact gtgtgccgac accagttttt ctaccaccag
     3001 cgggatccac ggcgattctt gccagatgat atcctgcgcc gccttatata agcgggtctt
     3061 ttccgccgga tcattcgttt tcagtgcctg agccaggaag tcatccacct gtttattgct
     3121 gtaaaacgcg gtattaaaca gcgtcggtgg ccagttttgc gaggcaaaca gcggcgatag
     3181 tgcccagtcg gcttcgccgg ttgaagccga ccagccagtg tagaacatcc gcacgccgct
     3241 ctctttttgc cctttacctt caacttctgc cgcccgctgt ccggcatcca tcgcggtcac
     3301 ctgggcttta atcccgacct gcgctaactg ctgctgggta aattgcagca ctttctgcgc
     3361 ggtgctgtgg ttatgtgacg accacagcgt ggtactgaaa ccgttgggat atcccgcctc
     3421 tttcagtaat tcgcgcgctt tcactggatc gtaaggccac ggtttataac tttgcgcgta
     3481 ggcgatactt ggcggtacca caccagtagc tggcgttgca tagcccgcaa aggcaacttt
     3541 caccagcgcc ggacggttaa tggcgtaatt cagcgcctca cggaccttcg ggttatcgaa
     3601 cggcttttgc gtcacgttca tactgatata acgctgcata attgacggac tggccatcaa
     3661 ctcgatattt ttgtttttct ccagcagtgt ggcctgctcg tagggaatgg ggaaagcaaa
     3721 ctgcgcttca ccggtttgca gcattgccgc gcgggtgttg ttatccgcca ccggacgcca
     3781 ggttatgctg tccagtttgg gcaatcctgg ctgccagtaa cccgcgaatt ttttcacctt
     3841 cacaaaatcg gtctgattcc aggtatccag ttcatacggt ccggttccca ccggatgaaa
     3901 accaatctcc ttgccatatt tttccagcgc tgtcggtgaa atcatcgcgg tcgccggatg
     3961 ggcaagaata ttaataaacg ctgagaacgg ctgtttgagg gtaatcttta ccgttgtcgg
     4021 atcgatcgcc tccgttttgg caatattctt atacaggtta tagcgtttaa gatgattcgc
     4081 cggatcgctg gcccggtcca gattcgcttt taccgccact gcgttgaaat cggtgccatc
     4141 ctggaattta attccttccc gcaatttcac ggtgtaagta atgccgtcat cggaaacggt
     4201 ataactctcc gccagcacgt ttttcagttt catctcttta tccagaccga acagcccctg
     4261 gtaaaacgat ttcgctacgg cctgagataa cgtgtcattc gcgtcatacg gatcgagcgt
     4321 ggtgaaattc gatcctaccg ccaccaccac atctttggca gcgaatgcac aagatgccat
     4381 caacgctgtc gcaatgccca gcgccactaa cccactacgg tgtacagctc ttgccatttt
     4441 attctccgcc tgaatgttat ctacgcatga atgcgtattc tgattgtggt tgtgcgacgt
     4501 aatgccccgg cccgacgcat tgcaacgaga cggctgccac ctcttcgcca cgcagatgaa
     4561 tattgctggg aagatcgtcc gacagcagta cacgctgcgg tcgttgtcgg gacggttcag
     4621 cgaccggaac tgccgccagt aatttacgcg tataaggatg ctgcgggttt tcgaagaccg
     4681 cgcaccgtga accaatttca acaatttgcc cgagatacat caccgccaca cgatgactaa
     4741 tccgctctac cacagccata tcgtgggaga taaacagata cgcaatgccg aaatcacgct
     4801 ggagatcgag caacaagttg ataatctgcc cgcgaataga aacatccagc gccgaaacgg
     4861 cttcgtcggc aatgatcact tttggattca atgccaacgc gcgagcaatg cagatgcgct
     4921 ggcgctgacc gccggaaaac tcatgcgggt aacgccaggc atgttcaggt aacaggccca
     4981 cgcgctccag caaccacgca acgcgtgcag ccgcgtcttt acctggcaat aaaccgtgta
     5041 cacgcagcgg ttcgataatc gaatcaccga tggtctgacg tgggtccagc gaagcgtaag
     5101 ggtcctgaaa aataaactga atatcccggc gcaatgcctg aagtttgcca ggtgacaagg
     5161 tatcgattcg ctgaccgtta aagataattt cgccgccctg cgattcgacc aggcgcagca
     5221 acgcccgccc ggtagtggat ttaccgctgc cagactcgcc caccagcgat agcgtttcgc
     5281 caggccagag atcaaaactg actttctcaa cggcatgcac ttcccgcgtt acgcgattca
     5341 acaaaccgct gcgcaaaggg aaacgggtga caagattacg cacccgtaaa acaggttcgc
     5401 catccaccac cgttttctgc tcgatggggg gttcctgttt cgctggatgt tcaagcgata
     5461 tcaatgggaa acgtcgggga taatctaacc ctttcatcgc accaagttgc ggaacagcag
     5521 ctaacagcgc acgggtgtaa ggatgttgcg gtgcatgaaa aatctgttcg acggtacccg
     5581 tttccaccgc ctcgccctga tacatcacca gtacccgatc ggcaatctct gccaccacgc
     5641 ccatatcgtg agtgataaag ataacgccca tcgacatctc tttttgcaat actttgatta
     5701 attgcaggat ctgcgcctga atagtgacat ccagcgcggt ggttggctca tcggcaatca
     5761 gcaccgccgg gcggcatgac agcgccatcg caatcatcac tcgctggcgc atcccgccag
     5821 agagttgatg cgaataacgt gaaagaatgg tttgtgcctc cggaatgcgt acctgatcca
     5881 gcatccgctt cgcctcgacc atcgcttctt cacgactggc gttctgatgc agacgaattg
     5941 attcggcaat ctgttcaccc acagtaaata ctgggttcag cgatgtcatc ggctcctgaa
     6001 atatcatcgc catatccgca ccgcgcacat ggcgcatttg tgcagcgctc tgctcgctaa
     6061 gttcaatcac atcacgactg cgccgccgca acagcatttt atcgcactgt actaaaccgc
     6121 ccgcctgttc caacagacgc atcaatgcca gcgcagtcac tgacttaccg gagccggatt
     6181 cgcctacaat tgccagcgtc tcaccgcgtt gcagactaaa agagagattg cggaccgcag
     6241 ctattttctg ctggtcctgc ataaaggcaa tattaagatt ttcaaccgcc agcacattac
     6301 cggcatcaag ttcatcactg tgtggcaacg gtgtcccctt tttcacggta gataccggtg
     6361 gttggcgtat cgcctgcgta gccccaggcg cgatacattc cttcggtgtt aaacggtagc
     6421 gcgacattcc cttcatggtc gatagcgatt aagccaccgc taccgccaag cgcagggagt
     6481 ttttccatta ctacccgctc gcaggcttcc gcgagactta atccgccgta atccattaac
     6541 gcggcgatgt catatgccgc cagcgcgcgg atgaagactt cgcctgtgcc ggtacaagaa
     6601 accgccacac tggcgttatt ggcgtagcat ccggcaccca ctaagggact atcgccaact
     6661 cgtccgggta atttattggt cattccgcct gtggacgtgg ctgccgccag attgccgtct
     6721 aaatccaacg ccacggcccc cacggtgccc attttctgtt tttcatccag tggcgcaccg
     6781 ctatggtcga ggactgttgc cccttcctcg cgcgctgcca gtagttgttc ataacgcaaa
     6841 ggcgtggaga aaatctccgg tgagacgcgc tccatgccat gagcaaacgc aaaattttct
     6901 gccccttcgc caatcatcat cacatgcggg ctttgctcca tcaccagccg ggcggcaaga
     6961 accggattac gcagatgact aacgcccgcc accgcaccgg ctttcagggt gttaccatcc
     7021 atcacacagg cgtccagttc atgggtttca tcacgcgtaa agacagcgcc aattccggcg
     7081 ttaaacagtg gacactcttc cagcagacgc accgcttccg tcaccacatc cagcgcactt
     7141 tcgccggctt ccagcatttt ctgcccggtt tcaacaatgg cagacaacgc ctcgatgtag
     7201 cgtaattcct gttgcagact catctgcgcg cggctaattg cacctgcgcc accatgaatt
     7261 gcaatgactg ctttgcccat agtattcgtc cattaagggt ggctcgccac ttttgctata
     7321 aatatcagaa tttttatgaa ttactcattc aatttttgag tatagaaaga tggatatggc
     7381 atgtaaaggc aggagccaac gctacgcccg aggtggcata cagtctatgg cgtttttctg
     7441 acataatagg caaattcgat tttgcctccg caggagtgtt ttcatggaat ttaccaccgg
     7501 attgatgtcg ctcgacaccg cgcttaatga gatgctttct cgcgttaccc cactgaccac
     7561 ccaggaaacg ctgccactgg ttcagtgttt tggtcgtatt ctggcgagcg atgtcgtttc
     7621 gccactggat gtcccggggt ttgataactc cgctatggac ggctacgcgg tgcgtttagc
     7681 cgatattgcc tccgggcaac cgctgcccgt tgccggtaaa tcctttgccg gtcagccata
     7741 ccatggtgaa tggcctgcgg gtacctgcat tcgtattatg accggtgcgc cggtgccgga
     7801 aggctgcgaa gcggtggtga tgcaggagca gactgaacaa acggacaatg gcgtgcgttt
     7861 tactgctgaa gtgcgtagcg ggcaaaatat tcgccgtcgc ggtgaagata tctctgcagg
     7921 tgcggttgtt ttcccggcgg gaactcgcct gactaccgca gagctgccag taattgcttc
     7981 actggggatt gccgaagttc cggtgattcg taaagtgcgt gtagcgcttt tttctaccgg
     8041 tgatgaactc cagttgcccg gtcagccgct gggcgacggc caaatctacg acaccaaccg
     8101 tctcgccgta cacctgatgt tagaacagct gggttgcgag gtaatcaact tagggattat
     8161 ccgcgacgat ccacatgccc tgcgcgccgc atttattgaa gccgacagcc aggcggatgt
     8221 agtgatcagt tccggcggtg tttcagtggg tgaagcggat tacaccaaaa cgattcttga
     8281 agagctgggg gagatcgcct tctggaagct ggcgattaaa ccaggtaaac cgttcgcatt
     8341 cggtaaactc agcaatagct ggttctgcgg cctgccgggc aacccgattt cagcgacgct
     8401 gaccttctat caactggtac agcctttgct ggcaaaacta agcggcaaca ccgccagcgg
     8461 cctgcccgcg cgccagcgcg tacgcacagc gtcccgcctg aaaaaaacgc caggacgtct
     8521 tgatttccag cgcggcgtgc tgcaacgcaa cgccgacggt gaacttgaag tgacgaccac
     8581 cggacatcag ggttcacata tatttagctc ctttagtctc ggcaactgct ttatcgtgct
     8641 ggaacgcgat cgcggcaatg tggacgtggg cgaatgggtg gaagtagaac cgtttaacgc
     8701 gttgttcgga ggcctgtaat ggcggaactc agcgatcagg agatgctgcg ctacaaccgg
     8761 caaatcattc tgcgcggttt tgattttgac ggtcaggagg cgctaaaaga ttctcgtgtg
     8821 ctggtagtcg gcctcggtgg actcggctgc gcagcttcgc agtatctggc aagcgccggt
     8881 gtcggcaacc tgacgctgct cgacttcgac acggtttcgc tctcgaatct gcaacgccag
     8941 acactgcaca gtgatgccac ggtcgggcaa ccgaaggtgg aatccgcccg tgacgccctg
     9001 acgcggatca acccacatat cgcgattacg ccagtcaatg cactgctaga tgacgcagaa
     9061 cttgcagcat tgattgctga acacgatctg gtgctcgact gtacggataa cgttgcggta
     9121 cgtaatcaac tgaacgcagg ctgttttgcc gcgaaggtgc cgctggtttc cggcgcggca
     9181 attcgtatgg aagggcaaat cacggtcttc acttatcagg acggcgaacc gtgctatcgc
     9241 tgccttagcc gtttgtttgg cgagaacgcg ttaacctgcg tagaagcagg cgtgatggca
     9301 ccgttgatcg gcgtaattgg ttcgttgcag gcaatggaag cgatcaaact gctggcaggt
     9361 tatggcaaac cggccagcgg gaaaatcgtc atgtacgatg cgatgacctg tcagtttcgc
     9421 gaaatgaaac tgatgcgcaa tccggggtgt gaggtgtgcg ggcagtaatt gccacctcca
     9481 ggcctgataa gacgcgacaa gcgtcgcatc aggcatttgc tccagacgcc agatgcggcg
     9541 tgaacgcctt atctggccta cggttcgggc acagatttcc aagcgcggca ggcatcggtg
     9601 cactccgcca cgtcatcagc aatttatgag gatgcagaac gtccggttaa atcgacgttc
     9661 tgccaaacgc tccctgccag tcctgctcaa acttcgccac agcggcttcc accgccggat
     9721 agctaatcat ctgttgtgcc acatccagtg gcagagtaat tgattcacat ccggccagta
     9781 agcagtccag cgcctgacgc ggggttttga aactcgctgc cagcactttc gcctgcggcg
     9841 catgcatttt caataactgg tgtaagtcgg tcacagtctg aatgccgcta ccgccctgag
     9901 catcaatacg attaacgtaa ggcgcaacat attccgcacc tgccagcgcc gacagcagcc
     9961 cttgtgccgc gccatatacc gcggttccca gcgtcggaat cccttccgct tttaacatct
    10021 taatcgctgc cagcccctcg gctgtcaccg gaactttcac cacgatatcc gcaataatag
    10081 aacgcagctt aagcgcgtca ttcaccatcc cttcagcagt ggtagccatt acctgggcaa
    10141 acagacgccc ctgaccgccc atcgcttcat gaagttgcgg aagcacaaca tccagcggtt
    10201 ttttacccgc ggcgataatg cttgggttag tggtcacacc cgccagcgga aaaatacgtg
    10261 acagcgcctt caccgcaaca acgtctgaag tatccagata cagttccata accatcctca
    10321 aaatgtactt tttgtctgaa tgtagactat cacgggaaaa ctctctgaac agttgacgca
    10381 catcaatcta actttcattc gaaagtattt taatgtttga acgaaacaga gaggcaacta
    10441 tgattttcaa tattcagcgc tactcgaccc atgatggccc cggtatccgc acggtcgtat
    10501 ttcttaaagg ctgttcgctg ggctgccgct ggtgtcagaa cccggaaagc cgcgcccgta
    10561 cgcaggatct gctgtatgac gcacgactgt gtctggaagg ctgcgagctg tgcgctaagg
    10621 ccgcgccgga agtgattgag cgcgcgctga atggtttgct tattcatcgg gaaaagttaa
    10681 ccccggagca tctgacggcg ttaaccgact gctgtccgac acaggcgtta accgtgtgtg
    10741 gtgaagtgaa aagcgttgag gagatcatgg cgaccgttct gcgcgataaa ccgttttacg
    10801 atcgcagcgg cggcggctta acgctttcgg gtggtgaacc cttcatgcag ccggaaatgg
    10861 cgacggcgct gctgcaagcc tgccacgagg caggcattca tactgcggta gaaacctgtc
    10921 tgcatgtgcc gtggaaatat atcgcccctt ctctgcccta tatcgatctg tttcttgccg
    10981 atttaaaaca cgttgccgac gcgccgttta aacagtggac cgacggtaac gccgccaggg
    11041 tgctggataa cctgaaaaaa ctcgccgcag cgggcaaaaa aatcattatc cgcgtgccgc
    11101 tgattcaggg ctttaatgcc gacgaaacct ctgtaaaagc cattaccgat tttgccgccg
    11161 acgagctgca cgttggcgaa attcattttc tgccctacca cacgctgggc atcaacaaat
    11221 atcacttact taatctgccc tatgacgccc cggaaaaacc gcttgatgcg ccagagctgc
    11281 tcgattttgc ccagcagtat gcctgccaga aagggttaac cgcgacctta cgaggataac
    11341 aaccatgacc acactgaaac tggacacgct cagcgaccgc attaaagcgc acaaaaatgc
    11401 gctggtgcat attgtgaaac cgccagtctg taccgagcgc gcgcagcact ataccgagat
    11461 gtatcaacaa catctcgata agccgatccc ggtacgtcgc gcgctggcac tggcgcatca
    11521 cctggcgaat cgcaccatct ggatcaaaca ctatgagttg atcattggca accaggcaag
    11581 cgaagttcgc gccgcgccga tcttcccgga atatactgtc tcgtggatcg aaaaagagat
    11641 tgatgatctg gcagatcgtc ccggtgctgg ctttgcggtg agcgaagaga acaaacgtgt
    11701 tctgcatgaa gtgtgcccgt ggtggcgcgg tcagacggta caggatcgct gctatggcat
    11761 gtttaccgat gagcaaaaag gtctgctggc gaccggcatc attaaagcgg aaggcaatat
    11821 gacctccggc gatgcgcacc tggcggtcaa tttcccgctg ctgctggaaa aagggcttga
    11881 tggtctgcgt gagaaagtag cggaacgtcg ctcgcgcatc aatctgacgg tgctggaaga
    11941 tttgcacggc gaacagttcc tgaaagcgat tgatatcgtg ctggtggcag tcagtgaaca
    12001 cattgaacgt ttcgctgccc tggcgcgtgc aatggccgcg accgaaaccc gcgaaagccg
    12061 tcgcgatgaa ctgctggcga tggcagaaaa ctgcgatctt atcgcccacc agccgccgca
    12121 gactttctgg caggcgctgc aactgtgtta cttcatccag ttgattttgc agattgaatc
    12181 taacggtcac tcggtgtcgt ttggtcgtat ggatcagtat ctctacccgt actatcgccg
    12241 cgacgttgaa ctgaaccaga cgctggatcg cgaacacgcc atcgaattgc tgcatagctg
    12301 ctggttgaaa ttgctggaag tgaacaaaat ccgctccggc tcccactcaa aagcctctgc
    12361 gggaagtccg ctgtatcaga acgtcaccat tggcggacaa aatctggttg atggtcaacc
    12421 aatggacgcg gtgaatccac tctcttacgc gatcctcgaa tcctgcggtc gcctacgttc
    12481 cactcagcct aacctcagcg tgcgttacca tgcaggaatg agtaacgatt tcctcgacgc
    12541 ctgcgtacag gtgatccgct gcggcttcgg gatgccggca ttcaacaacg acgaaatcgt
    12601 gatcccggaa tttattaaac tcggtattga accgcaggac gcttatgact acgcagcgat
    12661 tggttgtata gaaaccgccg tcggtggcaa atggggctat cgctgtaccg gcatgagctt
    12721 tatcaacttc gcccgcgtga tgctggcggc gctggaaggc gggcgtgatg ccaccagcgg
    12781 caaagtgttc ctgccacaag aaaaagcgtt gtcggcaggt aacttcaaca acttcgatga
    12841 agtgatggcc gcgtgggata cgcaaatccg ttactacacg cgtaaatcaa tcgaaatcga
    12901 atatgtcgtc gacaccatgc tggaagagaa cgtgcacgat attctctgct cggcgctggt
    12961 ggatgactgt attgagcgag cgaaaagtat caagcaaggc ggcgcgaaat atgactgggt
    13021 ttctggcctg caggtcggta ttgccaacct cggcaacagc ctggcggcag tgaagaaact
    13081 ggtgtttgaa cagggcgcga ttggtcagca acagctggct gccgcactgg cggatgactt
    13141 cgatggcctg actcatgagc aactgcgtca gcggctgatt aacggtgcgc cgaagtacgg
    13201 caacgacgat gataccgtcg atacgctgct ggctcgcgct tatcagacct atatcgacga
    13261 actgaaacag taccataacc cacgctacgg tcgtggtccg gttggcggca actattacgc
    13321 gggtacgtcg tctatctccg ctaacgtacc gtttggcgcg caaactatgg caacgccgga
    13381 cgggcgtaaa gcccacaccc cgctggcaga aggcgctagt ccggcctctg gtactgacca
    13441 tctcggccct actgcggtca ttggctcagt gggtaaactg cctacggcag cgattctcgg
    13501 cggcgtgttg ctcaaccaga aactgaatcc ggcaacgctg gagaacgaat ctgacaagca
    13561 gaaactgatg atcctgctgc gtaccttctt tgaagtgcat aaaggctggc atattcagta
    13621 caacatcgtt tcccgcgaaa cgctgctgga ggcgaaaaaa catccggatc agtaccgcga
    13681 tctggtagtg cgtgtcgcgg gttattccgc cttcttcacc gcgctctctc cagacgctca
    13741 ggacgatatc atcgcccgta ctgaacatat gctgtaatct cttcggcccg gctgcggcgt
    13801 cgccgggcta aatcattcac atcatcaatt tcatccttac tttcattcga aatataattt
    13861 gtgctctgcg tcacattgtt attacactgt tacggtttca ttttcgagcc aggagcacac
    13921 ctatgagcgt aaaagttatc gtcacagata tggacggtac ttttcttaac gacgccaaaa
    13981 cgtacaacca accacgtttt atggcgcaat atcaggaact gaaaaagcgc ggcattaagt
    14041 tcgttgtcgc cagcggtaat cagtattacc agcttatttc gttctttcct gagctaaagg
    14101 atgagatctc ttttgtcgcg gaaaacggcg cactggttta cgaacatggc aagcaactgt
    14161 tccacggcga actgacccga catgaatcgc ggattgttat tggcgagttg ctaaaagata
    14221 agcaactcaa ttttgtcgcc tgcggtctgc aaagtgcata tgtcagcgaa aatgcccccg
    14281 aagcatttgt cgcactgatg gcaaaacact accatcgcct gaaacctgta aaagattatc
    14341 aggagattga cgacgtactg ttcaagtttt cgctcaacct gccggatgaa caaatcccgt
    14401 tagtgatcga caaactacac atagcgctgg atggcattat gaaacccgtc accagtggtt
    14461 ttggctttat cgacctgatt attcccggtc tacataaagc aaacggtatt tcgcggttac
    14521 tgaaacgctg ggatctgtca ccgcaaaatg tggtagcgat tggcgacagc ggtaacgatg
    14581 cggagatgct gaaaatggcg cgttattcct ttgcgatggg caatgctgcg gaaaacatta
    14641 aacaaatcgc ccgttacgct accgatgata ataatcatga aggcgcgctg aatgtgattc
    14701 aggctgtgct ggataacaca tcccctttta acagctgacc tctctaccgg agccatgcgc
    14761 tccggttttc ccttcttttt acatcctgta catcacatca aatcattaaa cttgcattaa
    14821 cttatttata acttgtattg tcacatgcaa atcagcatag tttcgaatga aagttaatgc
    14881 gaggtggtta tggcttctac ttttaccagc gacacattgc ctgccgatca caaagcagct
    14941 atccgtcaga tgaagcacgc gctgcgggcg cagcttggcg atgtccagca gatctttaat
    15001 cagctaagcg atgacattgc cacgcgagtg gctgaaatca acgcactcaa agcacagggc
    15061 gatgccgtct ggccggtgct gtcttatgcc gatatcaaag caggtcatgt tactgcagag
    15121 cagcgcgaac agattaaacg tcgcggttgt gcggtgataa aaggccattt cccccgcgaa
    15181 caagcgctag gctgggatca gtcgatgctg gactatctga accgcaaccg ctttgacgag
    15241 gtctacaaag gccccggcga taatttcttc gggacgctca gcgcttcacg tcccgagatt
    15301 taccccatct actggtcgca ggcgcaaatg caggcccgcc agagtgaaga aatggcgaat
    15361 gcgcagtcgt ttctcaatcg tctgtggaca tttgaaagtg atggaaagca atggtttaac
    15421 ccggatgtga gcgtcatcta ccctgaccgt atccgccgcc ttccgcccgg aacgacctcc
    15481 aaaggtcttg gagcgcatac cgactccggg gcgctggaac gctggctgct tccagcgtat
    15541 cagcacgttt tcgccaacgt ctttaatggc aatctggcga aatacgatcc ctggcatgcg
    15601 gcacatcgta cggaagttga agagtacacg gtggacaaca ccaccaaatg ttccgtgttt
    15661 cggacattcc agggctggac tgcgctctcg gatatgctgc ctggtcaggg gttgctgcac
    15721 gtcgtgccca ttcctgaagc catggcgtac gtactgttac gtccgctgct tgatgatgtg
    15781 ccggaggatg aactgtgcgg cgtagcgccc ggaagagtgt tgccggtatc agagcaatgg
    15841 catccactgt taattgaggc gttaaccagc attccaaaac tcgaggccgg agactccgtc
    15901 tggtggcact gcgacgtcat ccattccgtt gcccccgttg aaaatcaaca gggttggggc
    15961 aacgtgatgt acattcctgc ggcaccgatg tgcgagaaaa atcttgccta cgcgcacaag
    16021 gtgaaggccg cactggaaaa aggcgcatcg ccgggcgact tcccgcgcga ggactatgaa
    16081 acaaactggg aaggacgctt tacgcttgcc gacctcaaca ttcacggtaa gcgagcgttg
    16141 ggcatggatg tttgagagtt aatggaagtg aattgatgtg ggattaagaa aatcgattcg
    16201 acgttactca gattgctgac aagtgcgctt tcttaatgcc tgatgcggcg ttaacgcctt
    16261 atccggccta caaagttctg cagattcaat atattacagg tactgcgtag gcctgataag
    16321 cgtagcgcat caggctattt tgcatttgtc atcagccgtc gtcaccccga cagtatcggg
    16381 gtgacggata atcacttact cgatcccttt actacgcagg taatcttcgt aattaccgct
    16441 aaagtcgatc acgcattccg gggtgatttc cagaatgcgg gtcgccaggg agcttacgaa
    16501 ctcacggtcg tgtgaaacaa agatcagcgt gccctgatac agttccagcg ccatgttcag
    16561 tgactcgatg gattccatat ccaggtggtt ggtcggttcg tccatgatca gaatgttcgg
    16621 cttctgcatc attaacttac caaacagcat ccgccctttt tccccaccgg aaagcacttt
    16681 agctggcttt ttgatgtcgt cctggctgaa cagcaaacga ccgagaatgc tgcgtaccgc
    16741 ctgctcgtca tcgccttcct gcttccactg actcatccat tcgaacacgg tcagatcatt
    16801 ttcaaactca tattcgtggt cctgggcgta gtaaccaatg cgcgcgtttt cagaccattt
    16861 tacggtgccg ctgtccggtt gcagatcgcc caccagcgtt ttcagcagcg ttgatttacc
    16921 gacgccgttg gtacccagta ccgcaagttt ttcaccgacc tccagcagca ggttgagatt
    16981 tttaaacagt ggaccgttat caaacccttt ggtcagacct tccacttcca gcgcgttacg
    17041 gaacagtttc ttatcctgtt caaaacggat gaacgggttc tgacggctgg aggctttcac
    17101 ctcttccagt ttgattttat caatctggcg cgcgcgcgaa gttgcctggc gagatttcga
    17161 ggcgttggcg ctaaagcggc taacgaaaga ttgcaactca gcaatctgcg ctttcttctt
    17221 ggcgttatcg gccagcagac gttcacgcgc ctgggtcgcc gccgtcatgt actcatcgta
    17281 gttacccgga taaacgcgca gctcgccgta atccagatcc gccatgtggg tacagaccat
    17341 gttaaggaag tgacggtcgt gcgagatgat gatcatggtg ctgtcacgct cgttcagcac
    17401 ctgttccagc cagcgaatgg tgtcgatgtc gaggttgttg gtcggttcgt cgagcaggag
    17461 aatatccggc tcagcaaaca gcgcctgcgc cagaagcaca cgcagcttcc agccaggagc
    17521 aacttcactc atcgggccgt agtgctgttc cactggaatt cccacgccaa gcaacagttc
    17581 accggcgcga gcttccgcag agtaaccgtc catttcgccg tatttcactt ccagatcggc
    17641 cactttatag ccgtcttctt cactcatttc cggcaaagca tagatgcggt cgcgctcctg
    17701 cttcacttcc cacaactctt tatgccccat gatcaccgta tccagcacag tgaacccttc
    17761 aaaggcaaac tgatcctgac gcagcttacc aatgcgctcg ttgggatcga gggaaacgtt
    17821 acccagcgtc ggctcaaggt cgccgccgag aatcttcata aaggtggatt taccactacc
    17881 gttcgcgcca atcaggccgt aacggttgcc gccgccgaat ttgacggaaa tgttttcaaa
    17941 caacggctta ctgccgaact gcatggtgac gttactggaa actaacacag gcgtatcctg
    18001 aaaagagata tgacaaaccg cgcattatgc cataagtctg aaagaagatc ctgctcctcg
    18061 cttaaggtct acactcaagc caaaggcaaa aaatgtgatt tcgtacacat ctgatttcac
    18121 tgtgagctgg aatgaactta taatgcgctt ccaatactct aatattctca acccaatggc
    18181 ctgccaggca caaaatctcg cttaacatga atatgaaatt gaaaacatta ttcgcagcgg
    18241 ccttcgctgt tgtcggcttt tgcagtaccg cctctgcggt aacttatcct ctgccaaccg
    18301 acgggagtcg cctggttggt cagaatcagg tgatcaccat tcctgaaggt aacactcagc
    18361 cgctggagta ttttgccgcg gagtaccaga tggggctttc caatatgatg gaagcgaacc
    18421 cgggtgtgga taccttcctg ccgaaaggcg gtactgtact gaacattccg cagcagctga
    18481 tcctgccgga taccgttcat gaaggcatcg tcattaacag tgcagagatg cgtctgtatt
    18541 actatccgaa agggaccaac accgttatcg tgctgccgat cggcattggt cagttaggca
    18601 aagatacgcc tatcaactgg accaccaaag ttgagcataa gaaagcaggc ccgacctgga
    18661 cgccgaccgc caaaatgcac gcagagtacc gcgctgcggg cgaaccgcta ccggctgtcg
    18721 ttccggcagg tccggataac ccgatgggtc tgtatgcact ctacatcggt cgcctgtatg
    18781 ctatccatgg caccaacgcc aacttcggta tcggcctgcg tgtaagtcat ggttgtgtgc
    18841 gtctacgtaa cgaagacatc aaattcctgt tcgagaaagt accggtcggt acccgcgtac
    18901 agtttattga tgagccggta aaagcgacca ccgagccaga cggcagccgt tatattgaag
    18961 tccataaccc gctgtctacc accgaagccc agtttgaagg tcaggaaatc gtgccaatta
    19021 ccctgacgaa gagcgtgcag acagtgaccg gtcagccaga tgttgaccag gttgttcttg
    19081 atgaagcgat caaaaatcgt tccgggatgc cggttcgcct gaattaatcg tcaaaacgta
    19141 aaacaaaggc ggactcataa tccgcctttt ttatttgcca gaccttacca ggccgggatc
    19201 ataactaaca aaacgtatcc caccagcgcc gcccacaaca gcatcggtat tgaatagaga
    19261 tggaaacgcc accagatgcg gcgatcgttc gccatacgta gcgcaatcaa atttgccagc
    19321 gatcctggta ataaaccaaa gccccccacg tttaccgccc atgccagtaa taaagacgga
    19381 ggtacataat tgagcagcaa aatggtactc ggcacgttac tgatcacctg cgataaaccg
    19441 actgccgtta accataaccc gggttcagat aaatgactca cgttacccaa cacgccttgc
    19501 aacgctggaa gctgggtcag taaatggacg tcgataaaca tcgccataaa caccagcagc
    19561 aacgtccagt ccacactcaa caccacgcga cgcgccagca gcacaaagcc cgccgccaca
    19621 atcaccagtc cccacagctc ttgtttgaac tccagcgccg tcagaaaaac gatatacagc
    19681 cccagacaat tccacaccag ccgcggtttc cactccggtg tttgcatccc cgtatggtat
    19741 tgcagcgcct ttccagggaa acaacaccag cacaacagca ggagtgtcag catcattgcg
    19801 ccagccagcg gtgccatttg ggcaataaat ccggcaaacg aaagaccaga acgtccccag
    19861 ataagaatat tttgcgggtt gccaattggc gtcagtagcg aaccagcgtt gactgccagc
    19921 gcctcaaaaa taatcagccg attaaccggg atctcacaca gtctttttag cgtgatagtc
    19981 agcggaacaa caataaacag cgcgacatcg ttggtcagaa aggtagaaag cagcgccgcc
    20041 gccagcacca taaacatcgc cagccgacgc tccgtagcaa agcggcgcac cattttgcgc
    20101 cccagcacat caaaataacc gcttaactcc acacctttgg tcagcagcat caggccgctt
    20161 aaggtgatga tggtgtgcca gtcgatagca gcaggccagg atttcggtgc aaagggcacg
    20221 aaaaagctta atccgatacc aacaagaatt aataactgaa aaaaacgatc gccttgcagc
    20281 gtgcgtaaaa aaggcaggct catttggcac cgtgtttctg ggtaaacaaa cgaaaagcgt
    20341 cgagcgtctc ttcactaaca tggtgctcca tgccttccgc gtcgcgacgg gcgatttccg
    20401 gactgacgcc caacaccaac aagaaatttt cgactatctg atgtcgctcg cggctttcct
    20461 gcgccagctt ctctccttct gccgttaaaa acacgccacg ccaggggatc atttcaatca
    20521 gccccatggt tgccagccgc ttaagcattt tagccaccgt cggttgcgaa actcccagac
    20581 gagcagccat gtccacctga cgagcttccc ccacttccct gatcaagtca gaaatcagct
    20641 caacgtaatc atcaataagc tcgcgccgat gcgcctctct gacctggcgg aacccttcaa
    20701 cgtgctcttc cacgttcact aactgcgtca ctttttttgc tgttggcgta cctgcgcgac
    20761 gactcattgt gcttcctcat tatggtgacg catacatagc gttctcattt cgtgcgcaca
    20821 ttgtaaacca gagttgcgaa ggtacaaaaa attaacgttt tagcaatagc tatataatat
    20881 agcctgtgct atatctgtat gtaatgcaat catccctcaa ggatcgacgg gattagcaag
    20941 tcaggaggtc ttatgaatga gttcaagagg tgtatgcgcg tgtttagtca ttctcccttt
    21001 aaagtacggt taatgctgct ctctatgttg tgcgatatgg tcaacaacaa accgcagcaa
    21061 gataaacctt cagataaata gcggcgtcgc ggtacgccgc ttcactcctg cttttatgca
    21121 ggcataacgc gttttggtct gaaaaaaacc actttttgtc ggatttgcaa tccccttcgc
    21181 aaaagatttg ttcgtcagta gttgacctga acggcggctc gctctatctt cttgcagccc
    21241 tgcgtatatt gcggctcgcg gatgcggacc cctttcccct cttcacgcac tcttgcaggt
    21301 attgaccctt gacgccaggg taagcacatg gcgtttgtta ccatagtggc atatgaattt
    21361 aaccctcaaa gaatcgcttg ttacccgtag ccgggtattt agcccgtgga ctgcgttcta
    21421 ctttttacag tcgctattaa ttaacctcgg cttaggttac cccttcagtt tgctctacac
    21481 cgctgcgttt acggctattt tgcttttgct atggcgaaca ttgcctcgcg tacaaaaagt
    21541 tctggtcggt gtcagttcgc tggtggcggc ttgttatttc ccttttgctc aggcctacgg
    21601 cgcgcctaat ttcaatacat tgctggcatt gcactccacc aatatggaag agtcgaccga
    21661 aatcctgacg atttttccgt ggtacagcta cctggtcggc ttatttattt ttgcgctcgg
    21721 cgtaatagca atcaggcgaa aaaaagagaa tgaaaaagcg cgctggaata ccttcgacag
    21781 cctgtgtctg gtattcagtg tggcgacatt ttttgttgct cccgtgcaaa acctggcctg
    21841 gggtggcgta tttaaactga aagatactgg ctatccggta tttcgttttg ctaaggatgt
    21901 catcgtcaat aataacgagg tgattgaaga gcaagaacgg atggcaaaac tttccggaat
    21961 gaaagatacc tggacggtca ctgccgttaa gccgaagtat cagacctatg tggtagtgat
    22021 cggtgaaagc gcgcgtcgcg atgccctcgg tgcctttggc ggtcactggg acaatacccc
    22081 gtttgccagc agcgttaacg gtttgatatt tgctgactac attgccgcca gtggctccac
    22141 gcagaaatcg cttggcttaa cgctcaatcg cgtagtcgat ggcaaaccac agtttcagga
    22201 taactttgtc accctggcaa atcgcgcggg cttccagacc tggtggtttt ccaaccaggg
    22261 ccaaatcggc gaatacgata ctgctatcgc cagcatcgcc aaacgtgcag atgaagtgta
    22321 cttcctgaaa gaaggtaatt ttgaagcaga taaaaacacg aaagacgaag cgttactgga
    22381 tatgaccgct caagtgctgg cgcaagagca ctcgcaaccg cagctgattg ttctgcatct
    22441 gatgggctcg catccgcagg cctgcgacag gacacaaggc aaatacgaaa cctttgtgca
    22501 atcgaaagaa acgtcgtgct atctctatac catgacgcaa acggacgatt tactgcgcaa
    22561 gctgtacgat cagttacgca acagcggcag cagcttctct ctggtttact tttctgacca
    22621 cggtctggcc tttaaagagc gcggcaaaga cgtgcaatac cttgcccatg atgataaata
    22681 tcagcaaaat ttccaggtgc cttttatggt catttccagc ggcgataaag cgcatcgggt
    22741 gattaaagcc cgccgctcag ccaatgactt cttaggcttt ttctcgcagt ggacgggaat
    22801 taaagcgaag gaaataaata tcaaataccc gtttatatct gagaagaaag ccgggccgat
    22861 atacatcacc aacttccagt tacagaaggt ggattacaac catctcggaa ccgatatttt
    22921 cgacccgaaa ccttaaaaac aaaaatccgc cccgagaggc ggatttttat atcaccaaag
    22981 tgattagaag cggtaaccaa caccggcaat ccaggtgcct acgtcaacgc tacgaatacg
    23041 gctctgctcg taagagaagt ccagagcaac gttttccatc gggttgaact gcagaccagc
    23101 accgtaggag aaaccgtagt cgctggtgtc gtgtttgtag gtcgggtatt cagtggtctg
    23161 gaatttacca taacccacac ccactacacc gtagatgctt gcccagtcgt taatgcggta
    23221 agccggacca gcagtgatgc cgtagtactg gtttttgttg tagtcaccag agcttgcagt
    23281 acggcttttc tcggtgtaag tgaaagaacc gatcacaccc agcgggctgt tgtcttcttc
    23341 atagcggtat ttcaggttga aaccgcccat tttgttcatt tggccctgag cgtcgctctg
    23401 tgcgtaaccg ccagttacgg tagaagtcgc agctacggaa gtgcctgcgg tgaaagccag
    23461 aactgcggcc agtgctgaaa gacatgcaat ttttttcata accacctcaa atgtgattca
    23521 aataagtcct aagttttaaa tatatcaaaa attaatggga aactcttcgc gatttgtgat
    23581 gtctaacggg ccatttcatg taacagaacg tttccataca ccgctatcca tctaaattta
    23641 aatcactttt tcagagaact gcgtaagtat tacgcatgtt ttccctgtca ttcatccaga
    23701 ttattcctaa tcaccagact aatgattcca tcaatcctgg cgcattttag tcaaaacggg
    23761 ggaaaatttt ttcaacaaat gctcaaccag cattgggtat atccagtaca ctccacgctt
    23821 tacttaagtc tagatatttg tgggagaaag gatgcctggt tcattacgta aaatgccggt
    23881 ctggttacca atagtcatat tactcgttgc catggcgtct attcagggtg gagcctcgtt
    23941 agctaagtca ctttttcctc tggtgggcgc accgggtgtc actgcgctgc gtctggcatt
    24001 aggaacgctg atcctcatcg cgttctttaa gccatggcga ctgcgctttg ccaaagagca
    24061 acggttaccg ctgttgtttt acggcgtttc gctgggtggg atgaattatc ttttttatct
    24121 ttctattcag acagtaccgc tgggtattgc ggtggcgctg gagttcaccg gaccactggc
    24181 ggtggcgctg ttctcttctc gtcgcccggt agatttcgtc tgggttgtgc tggcggttct
    24241 tggtctgtgg ttcctgctac cgctggggca agacgtttcc catgtcgatt taaccggctg
    24301 tgcgctggca ctgggggccg gggcttgttg ggctatttac attttaagtg ggcaacgcgc
    24361 aggagcggaa catggccctg cgacggtggc aattggttcg ttgattgcag cgttaatttt
    24421 cgtgccaatt ggagcgcttc aggctggtga agcactctgg cactggtcgg ttattccatt
    24481 gggtctggct gtcgctattc tctcgaccgc tctgccttat tcgctggaaa tgattgccct
    24541 cacccgtttg ccaacacgga catttggtac gctgatgagc atggaaccgg cgctggctgc
    24601 cgtttccggg atgattttcc tcggagaaac actgacaccc atacagctgc tggcgctcgg
    24661 cgctatcatc gccgcttcaa tggggtctac gctgacagta cgcaaagaga gcaaaataaa
    24721 agaattagac attaattaaa tttacatttc tgtatggtta tgcataacca tgcagaattt
    24781 ctcgctactt ttcctctaca ccgtctttat atatcgaatt atgcaaaagc atatttattc
    24841 cgaaaattcc tggcgagcag ataaataaga attgttctta tcaatatatc taactcattg
    24901 aatctttatt agttttgttt ttcacgcttg ttaccactat tagtgtgata ggaacagcca
    24961 gaatagcgga atacatagcc ggtgctatac ttagtctcgt taattactgg gacataacat
    25021 caagaggata tgaaattatg agtaccgcta aattagttaa atcaaaagcg accaatctgc
    25081 tttatacccg caacgatgtc tccgacagcg agaaaaaagc aacagtagag ttgctgaatc
    25141 gccaggttat ccagtttatt gatctttctt tgattaccaa acaagcgcac tggaacatgc
    25201 gcggcgctaa cttcattgcc gtacatgaaa tgctggatgg cttccgcacc gcactgatcg
    25261 atcatctgga taccatggca gaacgtgccg tgcagctggg cggtgtagct ctggggacca
    25321 ctcaagttat caacagcaaa accccgctga aaagttaccc gctggacatc cacaacgttc
    25381 aggatcacct gaaagaactg gctgaccgtt acgcaatcgt cgctaatgac gtacgcaaag
    25441 cgattggcga agcgaaagat gacgacaccg cagatatcct gaccgccgcg tctcgcgacc
    25501 tggataaatt cctgtggttt atcgagtcta acatcgaata aatccatcgc tgatggtgca
    25561 gaactttagt acccgataaa agcggcttcc tgacaggagg ccgttttgtt ttgcagccca
    25621 cctcaacgca cttatttagt gcatccatct gctatcccca gctgattaag taaatttttt
    25681 gtatccacat catcacacaa ccgttacata aagattgttt tttcatcagg ttttacgcta
    25741 aataatcact gtgttgagtg cacaatttta gcgcaccaga ttggtgcccc agaatggtgc
    25801 atcttcaggg tattgctcta taaatcgtgc atcacgtttt tgccgcatct cgaaaaatca
    25861 atgagttgca aaactggcac gattttttca tatatgtgaa tgtcacgcag gggatcgtcc
    25921 cgtggataga aaaaaggaaa tgctatgaag tctgtattaa aagtttcact ggctgcactg
    25981 accctggctt ttgcggtttc ttctcatgcc gcggataaaa aattagttgt cgcgacggat
    26041 accgccttcg ttccgtttga atttaaacag ggcgataaat atgtgggctt tgacgttgat
    26101 ctgtgggctg ccatcgctaa agagctgaag ctggattacg aactgaagcc gatggatttc
    26161 agtgggatca ttccggcact gcaaaccaaa aacgtcgatc tggcgctggc gggcattacc
    26221 atcaccgacg agcgtaaaaa agcgatcgat ttctctgacg gctactacaa aagcggcctg
    26281 ttagtgatgg tgaaagctaa caataacgat gtgaaaagcg tgaaagatct cgacgggaaa
    26341 gtggttgctg tgaagagcgg tactggctcc gttgattacg cgaaagcaaa catcaaaact
    26401 aaagatctgc gtcagttccc gaacatcgat aacgcctata tggaactggg caccaaccgc
    26461 gcagacgccg ttctgcacga tacgccaaac attctgtact tcatcaaaac cgccggtaac
    26521 ggtcagttca aagcggtagg tgactctctg gaagcgcagc aatacggtat tgcgttcccg
    26581 aaaggtagcg acgagctgcg tgacaaagtc aacggcgcgt tgaaaaccct gcgcgagaac
    26641 ggaacttaca acgaagtcta caaaaaatgg ttcagtactg aaccgaaata ataacgctac
    26701 acctgtaaag cgcactggca gttccctctc ccccctgggg agaggattag ggtgaggggc
    26761 gcaaacccgc tccggggcca ttaattaccc tgaatttgat tatttacacc acggtaacag
    26821 gaacaacata tgcagtttga ctggagtgcc atctggcctg ccattccgct tctgattgaa
    26881 ggtgccaaaa tgaccctgtg gatttcggtc ctcggtctgg ctggcggtct ggtaatcgga
    26941 ttgctggcag gttttgcacg caccttcggt ggttggatag ccaaccacgt cgcgctggtc
    27001 tttattgaag tgatccgcgg cacacctatc gtcgtccagg tgatgtttat ctatttcgcc
    27061 ctgccgatgg cgtttaacga cttacgcatc gacccattta ctgcggcggt ggtcaccatc
    27121 atgatcaact ccggcgcgta cattgcggaa atcacgcgtg gtgcggtgct gtctatccac
    27181 aaaggttttc gtgaagcagg actggcgctc ggtctttcac gttgggaaac cattcgctac
    27241 gtcattttac cgctggcact gcgtcgtatg ctgccgccgc tgggtaacca gtggatcatc
    27301 agcattaaag acacctcact gtttattgtt atcggcgtgg cggaactgac ccgtcagggg
    27361 caagaaatta ttgccggtaa cttccgcgcc cttgagatct ggagcgccgt ggcggtgttc
    27421 tatctgatta ttaccctggt gctgagcttt attctgcgtc gtctggaaag aaggatgaaa
    27481 atcctgtgat tgaatttaaa aacgtctcca agcactttgg cccaacccag gtgctgcaca
    27541 atatcgattt gaacattgcc cagggcgaag tcgtggtgat tatcgggccg tccggttccg
    27601 gtaaatcgac cctgctgcgc tgcatcaaca aactggaaga aatcacctcc ggcgatctga
    27661 ttgtcgatgg cctgaaggtt aacgatccga aagttgacga gcgcctgatt cgccaggaag
    27721 caggtatggt gttccagcag ttttacctct tcccgcatct gacggcgctg ggaaacgtca
    27781 tgtttggtcc gctacgcgtg cgtggcgcga acaaagaaga ggcggaaaaa ctggcgcgtg
    27841 agctgctggc gaaagtcggt ctggcagaac gtgcacatca ctacccttcc gaactttctg
    27901 gtggtcaaca gcagcgtgtg gcgattgccc gcgcgctggc ggtgaagccg aaaatgatgc
    27961 tgtttgatga accgacttcc gctcttgacc cggaactgcg ccatgaagtg ctgaaggtta
    28021 tgcaggatct ggctgaagaa gggatgacga tggtgatcgt gacccacgaa atcggttttg
    28081 ccgagaaagt agcttcgcgg ctgatcttta tcgacaaagg ccggattgcg gaagatggca
    28141 atccgcaggt gttgatcaag aacccgccga gccagcgctt gcaggaattt ttgcagcacg
    28201 tctcttaata agacacattg cctgatgcgc tacgcttatc aggcctacag gatatctggc
    28261 aacttattaa aattgcatga acttgtagga cggataaggc gttcacgccg catccggcaa
    28321 aaaagcccgc acgttgtcag caacctgctt aatatccctt cctccctttc acccgaaagg
    28381 gaggcacacc agattcctct catttaaaat cgcccctccc ccagcatcta tacttatctt
    28441 tttgctctgt tttctcactg gaggagtcat gcggtggatc ctgttcatcc tcttctgcct
    28501 gctgggcgca cctgcccacg cggtatccat acccggcgtt acaaccacaa cgacaacgga
    28561 ctcaacgact gaaccggccc cggaaccgga tatcgaacaa aaaaagcggc ctatggcgca
    28621 ctggcggatg tgctggataa cgacacctcg cgtaaagagt tgatcgacca gttgcgtact
    28681 gtcgccgcga cgccccctgc ggaaccggtc ccgaagatcg tgccgccgac gctggttgaa
    28741 gagcaaaccg tgctgcaaaa ggtcaccgaa gtcagccgcc attatggtga agccctttcc
    28801 gcccgcttcg ggcaacttta tcgcaatatc accggctccc cgcataagcc atttaatcca
    28861 caaaccttca gcaatgcgct gagccatttt tcaatgttag cggtattagt gtttggtttt
    28921 tactggctga ttcgcctgtg cgcactgccg ctatatcgca aaatgggtca gtgggcgagg
    28981 caaaaaaatc gtgagcgcag caactggttg cagcttccgg cgatgattat tggggcgttt
    29041 attatcgacc tgctgttact ggccctgaca ttatttgtcg gccaggtatt aagcgacaac
    29101 ctgaatgcag gtagtcgcac catcgctttc caacaaagtt tgtttctcaa cgcctttgcc
    29161 ctgattgaat ttttcaaagc cgtactacgc ctgatttttt gcccaaacgt ggcggagctg
    29221 cgcccgttca cgattcagga cgagagcgcc cgttactgga gtcgccgcct gagctggtta
    29281 agcagcctga ttggctatgg cctgattgtg gccgtgccga ttatctctaa tcaggtgaat
    29341 gtacagatag gtgcgctggc gaacgtcatc attatgctgt gcatgaccgt ctgggcgttg
    29401 tacctgatct tccgaaataa aaaagagatt acccagcatt tgctcaactt tgcggagcat
    29461 tcgctggctt ttttcagcct gtttatccgc gcctttgcgc tggtgtggca ctggctggca
    29521 agcgcctatt ttatcgtgct gtttttcttt tcgttgttcg atccgggcaa cagtctgaaa
    29581 tttatgatgg gtgcaacggt gcgcagcctg gcgattattg gtatcgcagc gtttgtttcc
    29641 ggtatgtttt cccgctggct ggcgaaaacc atcaccctct cgccacatac tcagcgtaac
    29701 tatccggagc tacaaaaacg gctgaatggc tggctttcgg cggcgctgaa aacggcgcgt
    29761 attctgaccg tctgcgtggc agtaatgttg ctgttgagcg catggggatt gttcgatttc
    29821 tggaactggc tgcaaaacgg cgcggggcag aaaaccgtag atatcctgat ccgcatcgca
    29881 ctcattcttt tcttctcggc ggttggctgg acggtgctcg ccagtttgat cgaaaaccgg
    29941 ctggcttcgg atattcatgg ccgcccgcta cccagcgccc gtacgcgtac cctgctgacg
    30001 ctgttccgta acgcgctggc ggtgattatc agtaccatca ccatcatgat tgtgttgtcg
    30061 gaaatcggcg tcaatatcgc gccattgctg gcaggtgccg gggcattagg tctggctatc
    30121 tcgtttggtt cgcaaacgct ggtaaaagat attatcaccg gggtatttat tcagtttgaa
    30181 aacggcatga acactggaga tttggtgact atcgggccgt tgaccggcac agtggaacgg
    30241 atgtcgattc gctccgtggg cgtgcgccag gataccgggg cgtatcacat cattccgtgg
    30301 tcttcgataa ccacctttgc taacttcgtc cgcggtattg gttcggtagt ggcaaactac
    30361 gatgttgatc gccatgaaga tgctgataaa gccaatcagg cactgaaaga tgcggtaacg
    30421 gaattgatgg aaaacgaaga aattcgcggg ctgattattg gtgaaccgaa ctttgccggg
    30481 attgtcggct taagcaatac cgcgtttaca ctgcgtgttt cgttcaccac gctgccactc
    30541 aaacagtgga cggtacgctt tgccctcgac agccaggtga aaaaacattt cgacctggcg
    30601 ggcgttcgcg cgccagtgca gacttatcag gtgctgcctg ctccgggcgc gaccccggct
    30661 gaaccgctgc cgccggggga gccaacgctt taacgttggc gattaacaaa acggcggcgc
    30721 tgctcgtcgt tcataaaggt ccaggcaata aagcgactct gcttttgccc ctgggccatc
    30781 tcttttttta ccaccttcac cgcgcccacg tcggtcagag cacgatacaa cggcggtaag
    30841 ttttcaccac gagataccag cgaggtaaac cacatcacct gcttcgcgaa gcctttgctc
    30901 tcttcaatca tctttttgat aaagttgact tcaccgcctt cacaccacaa ctcctgttgc
    30961 tgaccaccaa agttcagtgc atcgtctttg ttcagcccca ggttacggcg tttacgctca
    31021 ctacctgccc gcgccgcagc ggcggaatcg tggaatggcg ggttacacaa ggtcgcgtcg
    31081 tattgctcgt ttttgtggat gatgccgtta aaaatcgccc cactctcttt ttgccgacgc
    31141 agacgaatgg cgcggttaag ccccggatta gcactgataa tcgcctgcgc actgcttaac
    31201 gcctggctgc tggtttcact accggtaaaa cgccagccat attcatgtac gccaatcagc
    31261 ggataaatac agttcgcacc aacgccgata tccagaatgc tggcattcgc tggaattgtt
    31321 ccgcttgctt cagccagtaa atcggcaagg tgatgaatat aatccgcccg gcccggtact
    31381 ggtgggcaga gaaaaccgtc ggggatatcc cagttcgcta cggcgtaaaa atgggccagc
    31441 aacgccttat tgagcgcctt caccgccagc ggattggcaa agtctacgct ttgctcccct
    31501 gcgggtgtaa gcgtgaggaa ttgcctgagt tcaggattga cctgacaaag cgtggcgaga
    31561 tcgtagcggc tgtgatgacg gttgcgcgga tgcaaccccg gtttctgggc ggacatagca
    31621 ttctccttta agcagcggcg taagataccc gcataacgcg acagggtaaa tatctggctg
    31681 cgtttatcat tccccaaaac cttaacatgg ctatggttta tggcaattca tcataaaaat
    31741 cagcattatg catgtcattg atttgtatta ttttgtaaaa atatatcgtt caaaaaataa
    31801 gcatatctct gtcaattttt tgcgtcattt gcatcgcgca ggcgacctac acttatcagg
    31861 cactacccgt tgtatttatg aggatgtcat tatgaaaaag tgcctcacac tactgattgc
    31921 caccgtcctg agcggaatct ctctcacggc ttatgccgcg caaccgatga gtaacctgga
    31981 cagcggtcag ctacggcccg ccggtaccgt ttcggcaact ggcgcatcaa acctaagcga
    32041 tctggaggat aaactggcag aaaaagcgcg cgaacaaggc gcgaaaggtt atgtcattaa
    32101 ttccgcaggc ggaaatgacc agatgttcgg tactgcaacc atctacaaat aaccccacca
    32161 acgtaactgc attcgcctct ggatgcagtt ttcgcctgtt ttgtaatcaa aaaacatcaa
    32221 atatgaacgc aatgtaaata aatgtatttc tttttcgcgc aatgggtgat agaaaatcgc
    32281 gtcaagtgat aatgcttatc aaaattatta tcacttgcac aaacactatc acgggattaa
    32341 cagtggcatc gcatccgcag agatgctttc tcgtggcagt gaaaatttca acatataaga
    32401 aaaagtcacc tgcaaaatgg aaaacaatcg caatttccct gccagacaat ttcattcgct
    32461 cacgttcttt gccggtcttt gtattggtat tacgcctgtg gcacaggcac tcgccgccga
    32521 agggcaagct aacgcggatg acacgctggt tgtcgaagca tcaacgcctt cgctttatgc
    32581 gccacaacaa tctgccgatc cgaaattctc gcgtccggta gcggatacta cccgcacgat
    32641 gacggtgatt tctgaacaag tgattaaaga tcagggcgca accaacctta ccgacgcgct
    32701 caaaaacgtc cccggcgtg
//
LOCUS       DADMGC010000033        32437 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.033, whole genome shotgun sequence.
ACCESSION   DADMGC010000033 DADMGC010000000
VERSION     DADMGC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.033"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            249..1028
                     /gene="ipaJ"
                     /locus_tag="JNZ73_001516"
     CDS             249..1028
                     /gene="ipaJ"
                     /locus_tag="JNZ73_001516"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HAY8731840.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(1976..2905)
                     /locus_tag="JNZ73_001517"
     CDS             complement(1976..2905)
                     /locus_tag="JNZ73_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HAY8731841.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(3122..3358)
                     /locus_tag="JNZ73_001518"
     CDS             complement(3122..3358)
                     /locus_tag="JNZ73_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY8731842.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(3362..5263)
                     /gene="ipaA"
                     /locus_tag="JNZ73_001519"
     CDS             complement(3362..5263)
                     /gene="ipaA"
                     /locus_tag="JNZ73_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HAY8731843.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLIDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTSPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANKINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSQIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(5272..6270)
                     /gene="ipaD"
                     /locus_tag="JNZ73_001520"
     CDS             complement(5272..6270)
                     /gene="ipaD"
                     /locus_tag="JNZ73_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HAY8731844.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNKALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(6321..7412)
                     /gene="ipaC"
                     /locus_tag="JNZ73_001521"
     CDS             complement(6321..7412)
                     /gene="ipaC"
                     /locus_tag="JNZ73_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HAY8731845.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAENKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSRNSAASQIAGNIRA"
     gene            complement(7432..9174)
                     /gene="ipaB"
                     /locus_tag="JNZ73_001522"
     CDS             complement(7432..9174)
                     /gene="ipaB"
                     /locus_tag="JNZ73_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HAY8731846.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINKTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(9180..9647)
                     /gene="ipgC"
                     /locus_tag="JNZ73_001523"
     CDS             complement(9180..9647)
                     /gene="ipgC"
                     /locus_tag="JNZ73_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocator chaperone
                     IpgC"
                     /protein_id="HAY8731847.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(9704..10330)
                     /gene="ipgB"
                     /locus_tag="JNZ73_001524"
     CDS             complement(9704..10330)
                     /gene="ipgB"
                     /locus_tag="JNZ73_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgB"
                     /protein_id="HAY8731848.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(10346..10735)
                     /gene="ipgA"
                     /locus_tag="JNZ73_001525"
     CDS             complement(10346..10735)
                     /gene="ipgA"
                     /locus_tag="JNZ73_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HAY8731849.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(10748..12232)
                     /gene="icsB"
                     /locus_tag="JNZ73_001526"
     CDS             complement(10748..12232)
                     /gene="icsB"
                     /locus_tag="JNZ73_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HAY8731850.1"
                     /translation="MSLKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGDVTKDTEINYLFEKRLGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDVVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            12546..14162
                     /locus_tag="JNZ73_001527"
     CDS             12546..14162
                     /locus_tag="JNZ73_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HAY8731851.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFYEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESEHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKKLQKNDNGEPYKLPQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            14172..14534
                     /gene="ipgE"
                     /locus_tag="JNZ73_001528"
     CDS             14172..14534
                     /gene="ipgE"
                     /locus_tag="JNZ73_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HAY8731852.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            14534..14992
                     /gene="ipgF"
                     /locus_tag="JNZ73_001529"
     CDS             14534..14992
                     /gene="ipgF"
                     /locus_tag="JNZ73_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgF"
                     /protein_id="HAY8731853.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            15001..16116
                     /gene="mxiG"
                     /locus_tag="JNZ73_001530"
     CDS             15001..16116
                     /gene="mxiG"
                     /locus_tag="JNZ73_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HAY8731854.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKKIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            16124..16375
                     /gene="mxiH"
                     /locus_tag="JNZ73_001531"
     CDS             16124..16375
                     /gene="mxiH"
                     /locus_tag="JNZ73_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex protein
                     MxiH"
                     /protein_id="HAY8731855.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLARNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            16388..16681
                     /gene="mxiI"
                     /locus_tag="JNZ73_001532"
     CDS             16388..16681
                     /gene="mxiI"
                     /locus_tag="JNZ73_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HAY8731856.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            16687..17412
                     /locus_tag="JNZ73_001533"
     CDS             16687..17412
                     /locus_tag="JNZ73_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HAY8731857.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            17409..17936
                     /locus_tag="JNZ73_001534"
     CDS             17409..17936
                     /locus_tag="JNZ73_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HAY8731858.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            17878..18573
                     /locus_tag="JNZ73_001535"
     CDS             17878..18573
                     /locus_tag="JNZ73_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MxiN"
                     /protein_id="HAY8731859.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKR
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALSKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            18566..18973
                     /locus_tag="JNZ73_001536"
     CDS             18566..18973
                     /locus_tag="JNZ73_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa secretion machinery protein MxiL"
                     /protein_id="HAY8731860.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            18957..19385
                     /gene="mxiM"
                     /locus_tag="JNZ73_001537"
     CDS             18957..19385
                     /gene="mxiM"
                     /locus_tag="JNZ73_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HAY8731861.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            19515..20147
                     /gene="mxiE"
                     /locus_tag="JNZ73_001538"
     CDS             19515..20147
                     /gene="mxiE"
                     /locus_tag="JNZ73_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional activator MxiE"
                     /protein_id="HAY8731862.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            20134..21834
                     /gene="mxiD"
                     /locus_tag="JNZ73_001539"
     CDS             20134..21834
                     /gene="mxiD"
                     /locus_tag="JNZ73_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HAY8731863.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSRLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            21853..22920
                     /gene="mxiC"
                     /locus_tag="JNZ73_001540"
     CDS             21853..22920
                     /gene="mxiC"
                     /locus_tag="JNZ73_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HAY8731864.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSQASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            22933..24993
                     /gene="mxiA"
                     /locus_tag="JNZ73_001541"
                     /gene_synonym="virH"
     CDS             22933..24993
                     /gene="mxiA"
                     /locus_tag="JNZ73_001541"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiA"
                     /protein_id="HAY8731865.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            25006..25407
                     /gene="spaK"
                     /locus_tag="JNZ73_001542"
     CDS             25006..25407
                     /gene="spaK"
                     /locus_tag="JNZ73_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone SpaK"
                     /protein_id="HAY8731866.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            25411..26703
                     /locus_tag="JNZ73_001543"
     CDS             25411..26703
                     /locus_tag="JNZ73_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HAY8731867.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            26796..27152
                     /gene="spaM"
                     /locus_tag="JNZ73_001544"
     CDS             26796..27152
                     /gene="spaM"
                     /locus_tag="JNZ73_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface presentation of antigens protein SpaM"
                     /protein_id="HAY8731868.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIRQYKPKF"
     gene            27121..27999
                     /gene="spa32"
                     /locus_tag="JNZ73_001545"
     CDS             27121..27999
                     /gene="spa32"
                     /locus_tag="JNZ73_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HAY8731869.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIAANYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            27993..28874
                     /gene="spaO"
                     /locus_tag="JNZ73_001546"
     CDS             27993..28874
                     /gene="spaO"
                     /locus_tag="JNZ73_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SpaO"
                     /protein_id="HAY8731870.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            28879..29529
                     /gene="spaP"
                     /locus_tag="JNZ73_001547"
     CDS             28879..29529
                     /gene="spaP"
                     /locus_tag="JNZ73_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaP"
                     /protein_id="HAY8731871.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            29544..29804
                     /gene="spaQ"
                     /locus_tag="JNZ73_001548"
     CDS             29544..29804
                     /gene="spaQ"
                     /locus_tag="JNZ73_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HAY8731872.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            29804..30574
                     /gene="spaR"
                     /locus_tag="JNZ73_001549"
     CDS             29804..30574
                     /gene="spaR"
                     /locus_tag="JNZ73_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaR"
                     /protein_id="HAY8731873.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            30581..31609
                     /gene="spaS"
                     /locus_tag="JNZ73_001550"
     CDS             30581..31609
                     /gene="spaS"
                     /locus_tag="JNZ73_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein SpaS"
                     /protein_id="HAY8731874.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSVFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            31622..31864
                     /locus_tag="JNZ73_001551"
     CDS             31622..31864
                     /locus_tag="JNZ73_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731875.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10152 a         4673 c         6436 g        11176 t
ORIGIN      
        1 ccgattatgg acgtaaatac gacaaaaata tgtcagtatc aggcggtatt aattgctgat
       61 gctgatggac attaactctt aataaaaggg tggatgctga aaggttgtta tggtgacgga
      121 cgtgatgacg acggaaaaga gtatgaggtt ttggatttaa agttcgttcg gggaataagg
      181 acccggtcag ctaattctca tagtagttcg cattgctgga tgcaatactg tgctgcaatt
      241 actcgtcgat gtcggaacaa cggaagcctt gcaaacgggg atgtattcat acaggtgtta
      301 tgttatatgg ggttttattg cagggggcta taccaagaga atatatgata tctcatcaga
      361 ctgatgtgag ggtaaatgag aatagagtaa acgaacaagg ttgttttttg gcgaggaagc
      421 agatgtatga caattcttgt ggagctgcca gtttactctg tgctgcaaag gaattagggg
      481 ttgataaaat accacaatac aaagggtcta tgtccgaaat gacaaggaag agttctcttg
      541 atttggataa tcgttgtgag cgggatttat acttaataac tagtggtaat tataatccga
      601 gaatacacaa agataatatc gcggatgccg gttattctat gcctgataag atagtcatgg
      661 ctacgaggct tcttggattg aatgcttatg tcgttgaaga aagtaacatt ttttcgcaag
      721 taattagttt tatttatccc gatgcaaggg acttattaat tggcatggga tgtaatatag
      781 tacatcaacg agatgtgtta tcttctaacc aaagagtttt ggaagctgtt gctgtatcct
      841 ttattggagt tcctgtcggc ctccactggg tgttgtgtag acctgatgga agctatatgg
      901 atccagctgt aggagaaaat tattcttgtt tttcaactat ggaattaggt gcaagaagaa
      961 gtaattctaa ctttatagga tatactaaaa taggtatttc catagttata actaatgagg
     1021 ctttgtaaat tgtcgttttt tatctgtgaa ggagcttgaa tattttgggg cggggggggc
     1081 ataacaaatg atattactga gtacaaattt agctatattg ggcggattag ttgtgtggga
     1141 agacgaaatg tttctcttga aataagtgaa ctccctcaag gaatgattgt gtgcttaaat
     1201 atttaagtgc tggtaagttt gagaaggctg acatattttg cagagcaaac agtgattgac
     1261 ttggatgata tttttatttt agtcactcgt tgtaattgga gtatgcttta ttttatttga
     1321 aaatagatgt attttatacc cgcaatttaa actgtaagcc ctatgagggg tatggatatt
     1381 ataagttact ctttgactaa gcataacttt tggggttaga aaaataaatc tcttgttaat
     1441 aattaatatg attttaaaat caaacttgtt tataattata aaataatgta agtcggtcat
     1501 tttatgggaa gattaggaga ataaaacagt aattatattt gagaaatctg ctaatatatt
     1561 cttaaagctt ttttgatact gtgaacgaag tctgttaatg gtggattttt tgtttatcac
     1621 catgtaaaaa aattgagtag taaaagcgtt gactgctaat cctcgctgtt tttattgaaa
     1681 cgattaaaaa tacgctgaat attttcggcc agtcactcat cttcacataa aattttgacc
     1741 atcatgccgc atcccttagt tgcacaattc attttcaatg atatgaatat tactaatttc
     1801 accagataat tttctcgtgg tcagcagggt atgagttttc accgtaattt taaggaaaca
     1861 cagtgcgagg ggattgaata acagatagcc gacggaatta ccccaccggc tgaaagtcaa
     1921 gttcaacgaa gatttttatt atctgaattg ggcagtttac atcagtgttc gatgtttatg
     1981 aagacgatag atggcgagaa attatatccc gaatagcttc atctatttct ctctgaactg
     2041 ttttgtttat tttagataat gtaaatatta gagttctgtt tgttttattc tcttttcttt
     2101 ttatgtaagt ccgtttgtct ttagaaataa ataacgagtc aacttttagc gaagggtttt
     2161 gctttctgtt tttgcttttc tttatgatgt ttaaaatttc ttttttatat atgtccgggg
     2221 gcaaatttgt atcaaggtct tttatttctt cctttaatat tggtagtgta gaactaagag
     2281 attcattagc cttttcaagg cctttataat aattgaataa tatcttgtag tcattaaagt
     2341 taagttctga cgcgattgga aatagagata ttatttcctg tggaacgctt gctgcctgaa
     2401 aggcacgagt gactttcgcg cgagacagat tctctttttt ggctatgtct ttataggaca
     2461 tccctgatac tttcagaaaa ttaagaccaa taccaagttc tcggatgcta tgctcttttg
     2521 ctgtttgtat atcgtttgct agttttctgg catcaagagt agatatatat tcttttgaat
     2581 atagaacttc aagatctgct cctgcatata ttgcagatgc tcttctacga gtgccatcca
     2641 gaatttcaat tctaccatca atctcccttc ctattacagg gaagaattgt tgtagcttta
     2701 tagtttttat gatatcggct aatgattctt ctgttaggga ttcttgatcg cgtacattcg
     2761 ttttttggtt gacgaaggtt aaatcttgga tccttttatg aggaatcttg gctttgataa
     2821 aggaaacttt attaccagaa tggagtgtaa actctttctt ttggccttcc tttatactta
     2881 acaagtcgtt gcacaaatcc accatatcac accctgttta ttcatattga tttatattga
     2941 caaaagttaa atgcagtgtg agcaagattg tatgtaactg ttgatgatag tcaacggtag
     3001 tcttaaaaat tactatttta atagctggat tgatgcaatt tatctttatt ggataaaaga
     3061 ataatattct tgtggagctc tgatgtgaga gctccataga tgaaaaaatg cttatcaatt
     3121 actatattga ttttgtacta tttttgacta cagaaataag atctccaata gtaataatgt
     3181 gttctaatgc agattcatct atacgtagat tgaactcaaa actaatcata tccagcattt
     3241 caatcagaga aagagaatcg gcagaaagat cttcaataaa tttagtttct tcagatagtt
     3301 tgctgtacgc tataccatag caaaaggcca ctattgataa tattttttct tttatcatat
     3361 tttaatcctt attgatattc tttaatactt ttgataggga agtggttaca tcttttgctg
     3421 ctttatatat attattgttt tcagctgtaa tatcattgtt attttttaaa tcagatatat
     3481 catctgtaag taattctgca gaggtatcgt caatttgtga caataccttg ctgagggcgc
     3541 tagatacttc ttttgccttt tcatagatcg catgattttt atcaattgta tctgcgttgg
     3601 tttctgttac gtagtttatt ttatttgcat ctgtaacttt taatttgttg tttattctgt
     3661 gttctgttgt ggattttgta atgtcctttg atttttcctg taactctcgt tcttgtgttc
     3721 ctgatttagt tccaatgaga gatataagat tacttaggga gtttgttact ttttttgaag
     3781 cttcaaatat cgtttcccgt gttaaatatg tgtctccatt tcttgataaa aaatcagtct
     3841 gaagtacttt tgaatcagtg tttgagttga atttatttat ttgcgaagct actctatgag
     3901 aaagagaagc gctactttta gttgtatctg atgagttatc attactgctt gtcactcctt
     3961 ccgggtggag aggggaggtg gttgaagagt tctgtatgtt cagaggcctg tgtccccgag
     4021 aaagagagtt atttgcagat ctttcttgac tactcgtagt taacaggtct gaacctattc
     4081 catccgaatt atctggagta tggttgaaaa ccttttctaa agctgaatta tttgcaaatg
     4141 tctggacagt atctgatata gagtgtggta gaacggaagt tactttttga actagtgctc
     4201 tttgtggatt tagtaatgag tttccgttac gggataatag atcgtttgtc tgagaagtag
     4261 gagactgtgc atcattttct gggttctcgt gatagctatt gtcatatact ctgttgtcaa
     4321 aaactctatt atcgtaagtt ctattatcat tatttatatg atagtgaatt acaggttggg
     4381 atgttttctc attggctccg ggggttggtc cgccatctgg catatcttcg ggaggtgtag
     4441 ggaggccatc ttggatagga tgcgaatctt ttatatcttc taaaatatca ttaaggactt
     4501 ttctcaacag ctcaggaaac acggctactg tatttaagtc tcttgacagt ttacctatac
     4561 caaatccttt actatcaata gtatttttac gttgattgcg caaactatca aaggcaagtt
     4621 ttgcctgtgc gtcaattaaa tcatctagtt tgagagatga aacagtttca ttacttaact
     4681 ttaattcagc aagaatatta ttcttgataa atgatgatac ttcagtattg aggagttttt
     4741 gtcccaatga taaagcttca ccagatgtgt gttgttgtgc taatagtgca agtctgtgct
     4801 caatgttttg tcgtaattga tggagtttct tattctttgc cgaccatggt ccaaaggcag
     4861 aatcttggga taatttatct tttataatta gttccgaata ctgtgcgaca attttttgaa
     4921 gtcccttttc gttttcaaaa ttaagaccat tttgatgtac taaggagtac aaaacatttg
     4981 aaaattcgag aatctcgctt ttggcatacg cattcattga gttggaataa atgcttctta
     5041 acacatcttc tttccctgag gaagaaaata aaaaatcaag acatttctga ttaaaagacg
     5101 ttgcaaagtt ttctttttca gacactgatg ctggtgtttt tagagaagtt tgcgaactat
     5161 ggatatcgga tattttgctt tttaactcgc tgtattctgt tgatgatggt gaagttgcct
     5221 tatataagaa tgttggcgct tgagtattat ttacattatg catggcgcac ctcagaaatg
     5281 gagaaaaagt ttatctgtat ctgtacatga gcttattgta ctactcaaaa cctttactaa
     5341 attatcaaaa atactattgg cattactgta tttttgaact aaagtttgaa gattattttt
     5401 cattgtttca tcttcggcag agaatccggc attccatgcc tgatattttg cattatctag
     5461 cacaacctcg ccatttccac ctagattatc taagcttttt aacatattgt ctattggggt
     5521 catgtttata ctgacaacat atcccccgtt tttttgagat accttgccga ttgttccacc
     5581 taattctgta agccatttat ttgcttgttc ctgactaaca gtattatttg ctggatatag
     5641 cggtttatct ttatattttt ccttgagttc ttccaatgcc tttttaagcg agttgacttg
     5701 taatttcacg gagtttccgt cgttacctcc gggagagatc cagccggcaa gactggaaag
     5761 aacagcgcta aaatcttgat acatttgagt atatgaacta acggcatgtt catatacttt
     5821 cagatactgt tcattaatat cattgatgga gtttgcaatt ttagcccaca gttctctatg
     5881 agatatcatt tgatctccat caagctcggc ttctttcggg gctgaatgta ataattctct
     5941 tgcgtcttta ttaattggat attcgttcct ggaaagaata tccaatagtt gagcggattt
     6001 atttacatcc atgctaatct gagatgaatg taatgctatt tcttctagcg aagttttagt
     6061 caacgttttt tgtgaaagct ctttctttaa tgccttattt gttgttctga tattatgaag
     6121 ggtgtcgtta agcatagtaa gggaacttac aggatgagaa ctggtcgttg tttttatatc
     6181 agaattaact gtttcggttg atgaaccgtt ggtattgttt ggactgaatg atgaggtgga
     6241 aatactatta gtcagagttg ttatattcat ggttatttcc ttacaggaag agccatatat
     6301 tattatggct cttggatata ttaagctcga atgttaccag caatctgact ggctgccgaa
     6361 ttccttgatt gggtgatgct gtcaattata ttcaataatt tttgtattaa ttgatttgtc
     6421 gcttgggatg cttctttaga tacttgggat gcttcctctg cttgtttact gctggcctga
     6481 ctgattagtt gttcttcttc ttcaagagca gatgcataac gccctcctga tgtggatata
     6541 ttaccagcaa cggctgatgt ttccattgtt gcacggccaa tgttttgttt ttgctgcgca
     6601 gaaagggtat tgagctcgta agttcttctc tgggtgtcaa ttttatcctg caaagaaata
     6661 tcgggagaac taagagaagt tttatgttca gttgacaggg atatattatc tggcgccagt
     6721 ttatttttac ctaaaaattt tgtgctagag ttagtttgtg gtgaggtgat atttgtatct
     6781 atttgtttat ttaaccctaa tttagaacct gcaagctctt tttcaagaga ttgagcagtg
     6841 gcaaggttct ttcttaaggc tcctttttgg tcgctaatcc ctgaatgcgt ttttttagca
     6901 ccgatacccg ttatacctac ttgtgtgact gctccagtaa tgcttgatga tagggctgcc
     6961 aggccttgcc gaacaatgtt ctctgcagct gattttgtag catcgaacgc aatcaatgac
     7021 aattgagagc ccaatttatt ttctgcagtg cggagagcag aaagtagaac ggctacacta
     7081 ataattaaag aaacagcatt agaagaaaga ctggaaatat ccagagtgtt ctctggagta
     7141 agggtgtgac tcatctctga aagatctttc atctttttgt cagtaaggcg agcaatgatt
     7201 tctgaatcat gctgaacctg ctctgataac tttaaaagtt gatcatcatt taatgtggtt
     7261 gttaatacgg gatttttacc gacattaagt ggaatatgcg ctgcaatctg ctgataattt
     7321 tgtgattttt gtgtttcgga agaactttga gtttgttttg tggatatatc tgtatataaa
     7381 atctgggttg gttttgtgtt ttgaatttcc ataacattct ccttatttgt atcaagcagt
     7441 agtttgttgc aaaattgctt ttgcaacatc agttctatta gcctgagagt tggacattga
     7501 ggctaataga tctgcaatta cttcctgcaa ttggccgaat ttttctattg cttcactgat
     7561 atattttgac agttgttcaa cttgatattt cgataatgtc aggtctgcta gatttgtcga
     7621 cgcgctgttc tggaaaacag cagaagcgac acttcctccc gcttgtgtgg cagagttaac
     7681 actttctcct aataaaactg cttggtttaa atgggtggaa ataatttgtt tggatattac
     7741 ttcatcaccc gctgcaccaa gaaatttatt taatctggca acagcattag tgattaaatc
     7801 gtccagttga gaagaaaaat tcttgagaaa ctttggtata aggtctgtga gggttttacc
     7861 tattattttg ccaatattct ctgcaagttt tgctgctgcc tgtttaccaa cagtggctac
     7921 gagaacgact gctgcaacta ggacaagagc gcctgcgatt gcccccagaa tagagccaat
     7981 cattttggct tttttcgagt cgacgcccaa gccttcgagc atttttgtaa atgcatctga
     8041 aaggagtttg attaagggtt caatgactgc tttcatgatc ggattcaggg cttgttccat
     8101 gaaggaattg ccggtcgctg cttgtactat agcatccgta accataagag ctaaaccaac
     8161 agctgccagt gctagagagg ctcctccaga aaaagctgct gcaacaacac taacgatagt
     8221 taaaagtgcc ccaagtattt tcccaacaca acccattact ctgttgagtt cttctgcttt
     8281 acgtacttca gcagcatact catcagattt tctctccatt tcagtttttc ttgattcttg
     8341 gagagactgg aatagagcca gatcattttt taaagattct tcattatttt ttccaactag
     8401 ttgaataaag gttgccatca attgagtaac actggcaagt ccggttaatg atttctgctg
     8461 ggttgatagc tgttcagcag atgctgtgtt tgaaaatgca gaaaaagagt ctatttcttt
     8521 ttcgagttgc attgatttat ctgtaagttt gctatgaatt gacagggttt tctgctcaat
     8581 caatgtcctg tctttaactg ctgcgtcttt tttgatagtg agttgtattt cttcccggct
     8641 taattttttc ttttctggtg actctgggtc gagttcggat aatcttgttt gaatttggtt
     8701 aattttattt tctaggtctt ttattttaga atctgcgttt tttagtttat taatttgttt
     8761 ttcatagtct ctggttagtc cttcagtttc agatagaaga gtgttaattt tatcggagaa
     8821 ttctaggttt ttttgctgtc ttgcctgttg ctgggacttc caagcagtaa ttttatttgt
     8881 taatgcagtt aaagattttt caccgagtat ttgaataagg tttccaatta aaagcgttag
     8941 ttgggaactt gcatttaatg actttggtgc tttaagttca gggattaata tatttgaagt
     9001 tgagtgtgca ttagttttat taatattttg gttagcagta agatcagcaa ttgtaagaga
     9061 aaataattta ttagctgcat catttgcagc ttggatagta ttgtctccaa gctcagtgga
     9121 agccaatatt ttggcaagag gaaaaccagt ggttgtggtg cttacattat gcataataat
     9181 tactccttga tatcctgaat tgcgtccaag tatgattgtg cttttatttt taatttttca
     9241 tcattgctgt gttgaattac gagttcgaag cactctttag cttttaaggg ggctttcaac
     9301 cgaagctggc attgtccagt atggaatact ggtgtatagt cattttttcc taatgcaaaa
     9361 gcgacagcat aaaggtctgc tgcttgttgg aactgttctt ttatctgata aatagctgcg
     9421 agtcccataa tgtagtctac attgtaaaag tcgtatatac ataaaaatct gaagaaaact
     9481 tcagcttcct ctattcttcc tttgttgtaa aagtcataag catatgaata aatgtcatcc
     9541 atcatatcat caggaattgc attaatatct ttcagtgtag cgccagagtt aattgcatca
     9601 attactgcag tagagatgct ttcattttca gtgatattta aagacataag gtctcctttt
     9661 tgtctgcttt actttaataa gtataagatt taatataaaa gatttaattt gtattgcttt
     9721 gacggtatac agcctttaga acctctgcgc ttatttctat ttgtaaaact tttgttgatt
     9781 cattaaatat tttatcgtag acggaactgg atactagatt tgtaagatcc ttcatttgtg
     9841 tgttaaacaa atccatgtca ctgctatagc gttttaaaat ctttgctctg aggccagatg
     9901 aggcaatctc gtttagtgct gtatgactga tagatgtttg tgctgatttt tcatcaagtt
     9961 ttatatccgg gaattttttt tcaatagctg caaatatgtt ttttttgtta tctgaggtca
    10021 aaacaactcc attgtgtatg gccatttcat ctatacttct gtttatcatt gaagagacat
    10081 aagaggttcg ctcttggctc atccaacaaa atagttgatt ccccgaatta ctatctttta
    10141 ttttgtctat gacgctgtcc ctgattttat ttatattcac cgggcatcca aaattcttct
    10201 gaggaaagcg ttgttttaag ttaaaaacag ataatatttt ctttacaagc ttattgcgac
    10261 ttaacgaatc aaatgatggg cgaggaatcg cgaattcaac ctgtggaagt attttgttta
    10321 gaatttgcat gataccccct atatgttagt tcacttctga agtgatgttt gcaatggtta
    10381 gtttaataca aaataaaaat tcatacaaag attcaatgag ttcatcaggt gaaagatatt
    10441 ttttgtagtg tttcttggtc aaaacacatt gatgagtatc agggagaaga cagatagaag
    10501 aaccataatc tctactcaaa gagtagttaa ggatgagtat gtgttctgga ttaataatat
    10561 tttctgtaaa aagttcatgt tcattaagaa gtccggtcaa aaagatgtat tcatcattat
    10621 ctgttataca aacaggtatt tgttcttcga gcaatataaa ggcttgattt ttatcgttaa
    10681 aatcaagctt tgcacctgtt atttcataaa gtttatcata tagtttgcga cacatttcta
    10741 tggccttcta tatattagaa tgagagttat tcaataaaag cttattgatc tgtaataagt
    10801 cgtgaatgta aagtttggtt ccaagatctg gcgatttaag agaattgtaa taatcaataa
    10861 tttttattga tttttcgata atttcttctt ttttatctgt tgcttcggtt tttaattcgt
    10921 ttaatacacg agtcatattg ctgggtgctg attttactaa gtatgagtgg caagcgttga
    10981 tgaccacatc aaagtcttct gaagatacat tttcagtagg atgtttgtaa agtgaaattg
    11041 ataaaggctt gaatgttttt ggtgtgtgga taaggttaat tttattgaaa cttttaagat
    11101 agctgcgtcg taattgagtc aatctggaaa gatcaaaatc agattcaata tacttcatta
    11161 tttcgttata agactgattg agactttcaa tgcgttgcct aactttattt gcatacgcgt
    11221 atacatcgtt aggtgtagca accaatttaa cgtcagggag tggaaagtat atttcagaat
    11281 ttcctgcttt aagaacatta agtgccatac cagcacagtt ttcagattta ctgattaatt
    11341 tataatttgc caattgtctg aaagcatctg ctttcttttt gcatataaat tttgacatgt
    11401 ttgtttcatc aaggccaaaa aggttatggc tgattttacc atcctttgtt ttattatcac
    11461 ctgataatgg aatgtatact ttatcagcac tgacccccca ataaccatag gggtcttttt
    11521 tttgattagc tctgggaaaa aatatttttt tgttgttatt ttgatcttgt tttgaattta
    11581 ataagtttct aacctcttgg cctgcatcaa gtctttcggc tgtttggtct gaaatcatat
    11641 ttagtttgtc ttgcttatag gtatcgacac tgtaacctaa gcgtttttca aaaaggtagt
    11701 ttatttctgt atcttttgta acatccccgc cgggatacca gctgagatag gtatgagttg
    11761 gttcctgtag taaattgttt tttatactga tactagtatg ccctaaatgg gttatgcctc
    11821 tatttcccca gcaacgaaaa actaaatgtc tttcaagatt tttggctgta ccgatgccat
    11881 gaaaacgctc tgcggtattc aacattaatt tgatgtctct acctgtgaaa gagtgttgaa
    11941 ttgagttttt agctgcttca taggcggcaa acgcactttc tgcagatttt tcgctagcta
    12001 atgtattgag gaaaagcaat aaggattttt gattctctag tcgataattt tttagttgtt
    12061 catttaatat ctgtgaattt atctttgcat taatagtgct taatgttctg aaaaagagat
    12121 cctttaggtt gaatttctgt gctatcaata taggtccatg ctctgtatct tcaacgcgta
    12181 taggcccttt tgtattgctt gcgtcaatga aattgctaat tttgaggctc atactttatt
    12241 aactctccat tacttggtga ttgtggggat gtaaaatgta tttggcatat aatatattca
    12301 attaataaat ttagaaactt gagcctgtta acataatcaa attttctttt gctgtacata
    12361 atatgtacct cgtgagcata tgtagtgctc gtttcatcat gaaatcccac aagataaagt
    12421 gcctgatgta tcaggctcgg agtgttatag aaaaagagag aaccctgttg aataagtatc
    12481 ttttttgttg taaaacgagg tacaaaactg caatcaatgc cccatttatg actgaggata
    12541 attaaatgca cataactaat ttgggattgc atcaggtttc atttcaaagc ggagattcct
    12601 ataaaggcgc agaagaaacc ggaaagcaca aaggtgtaag cgtgatttca tatcaaagag
    12661 ttaaaaatgg agaaagaaat aaggggatcg aagcgcttaa tcgattatat ttacagaacc
    12721 aaacaagctt aacaggaaaa agccttttgt ttgcgcgtga cagagctgaa gttttttacg
    12781 aggcaataaa gttggcaggt ggtgatacgt caaaaattaa agccatgatg gaacgattag
    12841 atacctataa acttggtgaa gttaataaaa gacatattaa tgagcttaat aaagtaataa
    12901 gtgaagagat aagagcacag ctaggcatta aaaataaaaa ggaattgcaa acgaaaataa
    12961 aacaaatatt tactgattat ttaaataata agaattgggg accggtcgac aagaatatta
    13021 gtcatcatgg gaaaaattat ggttttcaat taacacctgc ctctcatatg aagataggga
    13081 ataaaaatat atttgtcaaa gagtataatg gaaaaggaat ttgttgtgct tctacgagag
    13141 agagtgagca tatcgcgaat atgtggcttt caaaagtggt ggatgacgag gggaaggaaa
    13201 ttttttcggg tatcagacat ggagttatat cagcctatgg attgaaaaaa aactcatctg
    13261 aaagagctgt tgctgctcgt aataaagctg aggagttagt aagcgcagca ttatatagca
    13321 gaccagagtt attatcacag gctttgtctg gtaaaacagt agatttaaag attgtttcga
    13381 cttctcttct gacgccgacc agtttaaccg ggggagagga gagtatgtta aaagatcagg
    13441 tgaacgcatt aaaagggtta aactctaaaa gggggaagcc aacgaaatta ttaattcgga
    13501 atagtgatgg tcttctgaaa gaagtaagcg ttaatctaaa agtggtgaca tttaactttg
    13561 gtgtaaatga attagcgcta aaaatgggct taggctggag gaatgttgac aagcttaatg
    13621 atgaatcaat atgttctttg ttgggggata atttcctcaa aaatggtgtg attggaggtt
    13681 gggctgcaga agcaatagaa aaaaatccac catgtaagaa tgatgtgata tatctggcta
    13741 accagataaa agagattata aacaagaagt tacaaaaaaa tgataatgga gagccgtata
    13801 aattgccaca aagaatgact ctattggctt atactatagg tgctgtacct tgctggaatt
    13861 gtaagagtgg gaaggacaga acaggtatgc aggatgctga aattaaaaga gaaataataa
    13921 gaaaacatga aacaggtcag ttttctcaat taaatagtaa attatcctca gaagagaaaa
    13981 gattattttc tactattcta atgaatagtg gtaatatgga aatccaagag atgaatactg
    14041 gtgttcccgg aaataaagtc atgaaaaaat tgccgctatc ttcgttagag ctatcttatt
    14101 ctgaaagaat aggggaccca aaaatatgga atatggtgaa agggtattcg tcatttgtat
    14161 aagaggaata tatggaagat ttagcagatg ttatttgccg agccttgggt atccctttaa
    14221 ttgatatcga tgatcaagca atcatgcttg atgatgatgt gctaatatat attgaaaaag
    14281 aaggagattc aataaattta ttatgccctt tttgtgcatt gcctgaaaat ataaatgatc
    14341 ttatatatgc gttgagccta aattactcag aaaagatatg cttagctact gatgatgaag
    14401 gcggaaatct aattgcgcga ttagatctta ccgggataaa tgaattcgag gatgtatatg
    14461 ttaatacaga atattatatt tcgcgtgtgc gttggctgaa ggatgaattt gcgcgaagaa
    14521 tgaaggggta ttaatgtccc gtttcgtatt tattttgctg tgttttatcc catatttagg
    14581 gagagctgat tgttgggata aggctggtga aaggtacaat attccatcaa gtttgttaaa
    14641 agcgattgcg gaaaaagaat ccggatttaa taaatctgca gtcaatgtta ataacaatgg
    14701 aagtaaagat tatggtataa tgcaaataaa tgactttcat tctaaaagac ttagagaaat
    14761 gggatattct gaggaaatgc taattagtca tccatgtctt tctgtacatt atgcagcaaa
    14821 gttattaaat gagtttatga tgatgtatgg aagagggtgg gaggctgttg gagcctataa
    14881 tgcaggaacg tctccgaaaa agaaaaaaga aagattaaag tacgcagagg atatctatag
    14941 acgctatttg cggatagcag cagagagcaa gcagaataat cgaaggatat aagaggattt
    15001 atgtctgagg caaagaactc aaatcttgca ccattcaggt tattagtgaa actcacaaac
    15061 ggtgtcgggg atgagtttcc gttatattat gggaataact taattgttct cggacggacg
    15121 atagaaacac ttgagtttgg aaacgacaat ttcccagaaa atattattcc tgtaacagat
    15181 agtaaatcgg atggtataat ttatttgacc ataagcaaag ataatatctg tcaatttagt
    15241 gatgaaaaag gtgagcaaat tgatatcaat tcacaattta attcatttga atatgacggg
    15301 atttcttttc accttaaaaa tatgagggag gataaatcga gaggccatat actaaacgga
    15361 atgtataaga accactcggt gttttttttc tttgctgtta ttgttgtgtt aattataatt
    15421 ttttccctct ctttaaagaa agatgaagta aaagaaattg ctgaaattat agatgataag
    15481 agatatggaa tagtcaacac tggacagtgc aattatattc ttgccgaaac acaaaatgat
    15541 gcggtatggg cgtctgtagc cttaaataaa accggattta ccaaatgcag atatattctg
    15601 gtgtcaaaca aagaaataaa tagaattcaa caatatatca atcaacgatt cccatttatc
    15661 aatttgtatg tgttgaattt agtgagtgat aaagcggaat tactggtgtt tttaagtaaa
    15721 gaaagaaata gttcaaaaga taccgaactt gataaattga aaaatgctct gattgttgaa
    15781 tttccgtata tcaaaaatat taaattcaac tatcttagcg atcataatgc aaggggggat
    15841 gctaaaggta ttttcacaaa ggtaaatgtc cagtataaag agatttgtga aaataataaa
    15901 gttacatatt ctgtcagaga agaactgaca gatgaaaagc ttgagctcat taatagactg
    15961 atttctgaac ataaaaaaat atatggagat cagtatattg agttttctgt acttttgata
    16021 gatgatgatt ttaaaggtaa atcatatctt aacagcaaag acagttatgt aatgttgaat
    16081 gataaacact ggtttttttt agataaaaat aagtgaggat aaaatgagtg ttacagtacc
    16141 gaatgatgat tggacattga gttcattatc tgaaactttt gatgatggaa ctcaaacatt
    16201 acaaggtgaa ctaacattgg cactagataa attagctaga aatccttcga atccacagtt
    16261 gctggctgaa taccaaagta aattatctga atatacatta tataggaacg cgcaatccaa
    16321 tacagtgaaa gtgattaagg atgttgatgc tgcaattatt caaaacttca gataataggg
    16381 agcattcatg aattacattt atccagtcaa tcaggttgat attatcaaag ccagtgattt
    16441 tcaatctcaa gagatatcaa gtctggaaga cgtcgtgtcg gctaaatata gtgatattaa
    16501 gatggataca gatattcaag tatcacaaat aatggagatg gtaagcaatc cagaatcatt
    16561 aaacccagaa tctttggcca agttacagac gacgctctca aattattcaa taggagtatc
    16621 attagctggc acgttagcaa gaaaaacagt ttcggctgtt gaaactttat taaagtctta
    16681 atttatatga ttaggtataa aggttttatt ttattcttgt tgctgatgtt gattggatgt
    16741 gagcaacgtg aagagttaat ttctaattta tctcaaagac aggcaaatga aataatatct
    16801 gtgctagaac gccataatat tactgctaga aaagttgatg gaggtaaaca ggggatctcg
    16861 gtacaagtcg aaaaggggac atttgcatcg gcagttgatt tgatgcgcat gtacgatttg
    16921 ccaaatccgg agagagttga tatctcacaa atgtttccta cagattcatt agtgtcttct
    16981 ccaagagctg aaaaggcccg tttatatagt gctattgagc aacggctgga acagtcttta
    17041 gtttctattg gtggtgttat ttcggcaaaa atacatgtta gctatgatct tgaagaaaaa
    17101 aatatatctt caaaaccgat gcatatatca gtaatcgcta tatatgactc accgaaagag
    17161 tctgaactat tagttagtaa tattaagcga tttttgaaaa acaccttttc tgatgttaag
    17221 tatgaaaata tatctgtcat attaactccg aaagaagaat atgtttatac aaatgtacaa
    17281 cctgttaagg aagttaaatc ggaattttta acaaatgaag taatatattt atttctcggg
    17341 atggctgtac tagttgtcat tcttttggta tgggcattca aaacagggtg gttcaagaga
    17401 aacaaaatat gataagaatg gatggaattt ataaaaaata tctttcaata atttttgatc
    17461 cagcgttcta tataaataga aatcggttga atttgccttc tgaactgtta gaaaatggcg
    17521 taatcagaag tgagattaat aatctcataa ttaataaata tgatctaaat tgcgatattg
    17581 aacctttaag cggggtaacc gctatgtttg ttgccaactg gaatttactt ccagctgttg
    17641 cttattttat cggtagtcaa gagtctcggt taattaatca tagtgagatg gttattagtt
    17701 attatggggg caaaatcagt aagcaagggg aggccgctat tcgaagtggt ttttggcatc
    17761 ttattgcgtg gaaggaaaat atatctgttg gtatatatga aagaataaac ttattattta
    17821 atccaattgc tttggaggga aattatacgc ctgtagaaag aaatttgtcc cgattaaatg
    17881 aaggtatgca atatgcaaaa aggcacttta ccggtatcca gacatcatgc ctatgatggt
    17941 gttgttataa agagaattga aaaggaatta tgtaaaacca taaaagatag agacactgag
    18001 agtaagagga aagcaatttg tgttataaag gaggctacga aaaaagctga atcactgcgt
    18061 atagatgctg tatgtgatgg ttatcaaatt ggtatacaga ctgcttttga gcatattatt
    18121 gactatattt gtgaatggaa gttaaagcaa aatgaaaaca gaagaaatat tgaggactat
    18181 ataactagtt tgctttcaga aaatttacat gatgagagga tcattagcac tttattggag
    18241 cagtggctta gttcgttgcg taatacagta acagaattaa aagtggtttt gcccaagtgt
    18301 aatttagcac tgagtaaaaa gttagaactt gatctccata aatatcgctc agatgtcaag
    18361 attattttaa aatattctga agggaataat tatatattct gtagtggtaa tcaagtagtg
    18421 gagtttagtc ctcaggatgt aatatcaggt gttaaaatag aacttgcgga aaaattaaca
    18481 aaaaatgata aaaaatattt caaagagtta gcacataaga aactgagaca aattgcagaa
    18541 gacttgttaa aagagaatcc tgttaatgat taatcaaata aatgcaagca atgctctgca
    18601 gcaaagatta aatagtgaag aatttgttaa tttaaatgag agattatcat cttcgcaatc
    18661 atttgatgag gatataatct atgaaataat gcaatatttc tcacagagtg aattaaattc
    18721 catagataat gatgaactgc acaataagat tgaacaatta ttcaattcac gttttcctta
    18781 tcttacagct gcacaaaaaa gttcacttct taataaactc atagatgcaa atcaatatgt
    18841 cgatttgcat gagggatttt atgcatcatt gtccatatat aacaatattg atttctatat
    18901 taagactaca accttcgatt cattaattag tgtctttgaa gcagggagag aggcagatga
    18961 ttcgacatgg tagtaataag ttgaaaatat ttattttaag tatattgcta ttaacactga
    19021 gtgggtgtgc tttaaagtca tcatctaatt ctgaaaaaga atggcatatt gttcctgtaa
    19081 gtaaggatta tttttctatt ccaaatgatt tattatggtc gtttaataca accaataaaa
    19141 gtataaatgt ttactctaaa tgtattagtg gtaaggcggt ttatagtttt aatgcaggta
    19201 aattcatggg caactttaat gttaaggaag tagatgggtg cttcatggat gcacaaaaga
    19261 tagctataga taaactattt tctatgctga aagacggggt tgttttaaaa ggtaataaga
    19321 taaatgatac catccttata gagaaggatg gggaagttaa attaaaatta attcgaggga
    19381 tataattgta ttgtgagtaa atataaaggt ctaaatacaa gtaatatgtt ttacatttac
    19441 tctagtggac atgaaccagt taacgttgag cttgtaaaag ataaagaacg taacataatt
    19501 gagctggctc cagcatggaa gggttttttt ttgtccgaaa tcaaaatata aaattcagtg
    19561 ataatgtgaa ctatcattat cgctttaaca ttaatagttg tgcaaaattt cttgcttttt
    19621 gggactattt cagtggtgct ctagtagagc atagccatgc tgaaaaatgt attcatttct
    19681 atcatgagaa tgatcttcgg gatagttgta ataccgaatc gatgcttgat aagttaatgt
    19741 taagattcat cttttcatct gaccagaatg taagtaatgc actggctatg atacgaatga
    19801 ctgagtcata tcatttggtt ctttatcttc tgagaactat tgagaaagaa aaggaggtaa
    19861 gaataaagag tctgacagag cattatggtg tgtcggaggc ttattttaga tctttatgta
    19921 gaaaagcttt aggggcaaaa gtcaaagaac aactgaacac ttggcggtta gtaaatggtt
    19981 tattagatgt atttttgcac aatcaaacaa tcacttctgc tgccatgaat aatgggtatg
    20041 catcaacatc ccatttttca aatgagataa aaacacgact aggcttttct gcaagagaat
    20101 tatcgaatat tactttttta gtgaaaaaaa taaatgaaaa aatttaatat taaatctttg
    20161 actctcttga ttgtattgtt accattgata gttaatgcta ataatattga ctctcgttta
    20221 ttagaacaaa atgacatagc gaaatatgtt gctcaaagtg atacggtagg atcttttttt
    20281 gaacgttttt ctgcgttatt aaattatcct attgttgtta gcaaacaagc tgcaaaaaag
    20341 agaatatctg gggagtttga tttatctaat ccagaagaaa tgcttgaaaa actgacatta
    20401 cttgtgggac tcatatggta taaagatgga aatgcattat acatatatga ctctggagaa
    20461 ttaataagca aagttatttt gctggagaat atatcactta attatcttat tcagtatctg
    20521 aaggatgcta acctttatga tcacagatat cctataagag gaaacattag tgataaaaca
    20581 ttctatatat ctggtcctcc ggcattggtt gaactggtag ctaatacagc aactttgctt
    20641 gataagcagg tttcttctat tgggacagat aaagttaatt ttggggttat aaaattaaaa
    20701 aatacatttg ttagtgacag aacttataat atgagagggg aggatattgt catcccaggg
    20761 gtggctactg ttgtagaacg tttattaaat aatggtaagg ctctgagtaa cagacaagcc
    20821 caaaatgatc cgatgccacc ctttaatatt acacagaaag tatctgagga tagtaatgac
    20881 ttttcattta gttcagttac caatagttct atattagagg atgtatcact tattgcatat
    20941 ccagaaacca attctatatt agtcaaaggt aatgatcagc aaatacagat tattcgcgat
    21001 attatcactc agttagatgt agccaaacga catatagaat tatcattatg gataatcgat
    21061 attgataaat ctgaattaaa taaccttggt gttaattggc aaggaacggc ttcatttggt
    21121 gattcgtttg gtgcgtcatt taatatgagc tcatcagcat cgataagcac tttggacggc
    21181 aataagttta ttgcttcagt tatggcatta aatcagaaaa agaaagcaaa tgtagtttca
    21241 aggccggtga ttttgacaca agagaatatt ccggctatat ttgataataa caggactttt
    21301 tatgtgtcat tagttggtga gcgcaattct tcgttggagc atgttacgta tggaacattg
    21361 ataaatgtta tacctcgctt ttcatctcgg ggacaaattg agatgtcttt aacgatagag
    21421 gatggaacag gaaatagtca gtcaaattac aattataata atgaaaatac atctgtcctt
    21481 ccagaagttg gaaggaccaa aatcagtaca atagcgagag ttccacaagg caaaagtttg
    21541 ttgataggtg gttatacgca tgaaacgaac tctaacgaaa tagtatctat tccttttctc
    21601 tcctctattc ctgtaatcgg taatgtgttc aagtataaaa caagtaatat cagtaatatt
    21661 gtaagggttt ttctgattca accccgtgag ataaaggagt caagttatta taatactgca
    21721 gaatataaaa gcctcatatc tgaaagagaa atacaaaaga ctacacaaat aatcccatct
    21781 gaaactactt tgctggaaga cgaaaaatca ttggtttcat acttaaatta ctaactataa
    21841 agtaggtgat gtatgcttga tgttaaaaat acaggagttt ttagctctgc attcattgat
    21901 aagttgaatg caatgacaaa ttcagatgat ggagatgaga ctgctgatgc agagcttgat
    21961 tctggcttgg ctaatagcaa gtatattgac tcatctgatg agatggcttc cgctctttcg
    22021 tcatttataa acagaagaga ccttgagaaa ctgaaaggaa caaatagtga tagtcaggaa
    22081 cgtattttag atggggaaga agatgaaatt aatcacaaga tttttgattt aaagagaacg
    22141 ttaaaagata accttcctct agatcgggat tttatagaca gactaaagag atattttaaa
    22201 gatccaagtg atcaagtctt agcattaagg gaacttttga atgaaaaaga tcttactgct
    22261 gaacaagtcg aattattaac taaaattatt aatgagataa tatcaggtag tgaaaaaagt
    22321 gttaatgctg gaataaattc agctatacag gctaaattat ttggcaacaa aatgaaactt
    22381 gaaccacagc ttttgcgtgc atgttatcgt ggttttatca tggggaacat atcaacaaca
    22441 gatcagtata tagaatggct tggtaatttt ggttttaatc acagacatac aattgtgaat
    22501 tttgtagagc agtcactgat tgtagacatg gattctgaga aaccgagctg taatgcttat
    22561 gagtttggtt ttgtgttatc taaattaatt gcaattaaga tgattagaac ttcagacgta
    22621 atttttatga agaaactgga atcctcaagc ttgctaaaag atggcagttt aagtgcagag
    22681 cagctattgc taactttatt atatattttt caatatccaa gtgaaagtga gcaaattctt
    22741 acttctgtta tagaagtatc acaagccagt catgaggatt ctgtagtgta tcaaacatat
    22801 ctatcttctg ttaatgaaag tcctcatgat atatttaaaa gtgaaagtga aagagaaatt
    22861 gcgatcaata ttctacgaga gcttgtcaca agtgcataca agaaagagct ttctagataa
    22921 caggagataa aagtgatcca gtcttttctt aagcaagtaa gtactaagcc tgaattaatt
    22981 atattagtgc ttatggttat gatcattgct atgttgatca taccattacc tacatacctt
    23041 gttgattttt taatagggct taatattgta cttgctattc tcgtttttat gggatcattt
    23101 tatattgaaa gaatattgag tttttctaca ttcccttcgg ttttgttgat aacgacatta
    23161 tttcgtctcg ctttatcaat aagtactagt cgactaattt tagttgatgc ggatgcgggt
    23221 aaaattatta ctacatttgg ccagtttgtc attggtgata gtctggctgt aggttttgtt
    23281 attttttcaa tagtaactgt tgtgcagttc attgttataa caaaaggatc tgaaagggtt
    23341 gcggaggtag ctgctcgctt ttctcttgac ggtatgccgg ggaaacaaat gagcattgat
    23401 gcggatttaa aggctggaat tattgatgcc gcaggagcta aagaaagacg cagtattctg
    23461 gaacgtgaga gtcaattgta tggttcattt gatggagcaa tgaagttcat taaaggtgat
    23521 gcaattgctg gcatcattat tatttttgtc aatttaatag gaggtatttc tgttggtatg
    23581 agtcaacatg gaatgtccct atctggtgca ttatctactt ataccatact aactattggt
    23641 gacggattgg tctctcagat tcctgctctg ttaatttcta taagtgcagg atttatcgtt
    23701 acaagagtta atggtgatag cgataatatg ggacgtaaca taatgtccca gatatttggg
    23761 aatccttttg ttctcattgt tacatcagct cttgccttgg cgatagggat gttgccaggt
    23821 tttccgtttt ttgttttttt cctgatagca gttactttga cggctttatt ctattataaa
    23881 aaggtcgtag aaaaagaaaa aagtctgtct gagtctgatt ctagtggcta tactggtaca
    23941 tttgatattg ataatagcca tgactcatct ttggcaatga tagaaaatct ggatgctatt
    24001 agttcagaaa ccgttccttt aattttatta tttgccgaaa ataagataaa tgcaaatgat
    24061 atggaaggtc ttattgaaag gataagaagt cagttcttta ttgattatgg tgtcaggctt
    24121 ccaactattt tatatagaac aagcaatgag cttaaggtcg atgatattgt tttgttaata
    24181 aatgaggtgc gtgccgatag ttttaatata tattttgaca aagtgtgtat tacagatgaa
    24241 aatggagata tagatgctct aggtattcct gttgtttcaa cttcatataa tgaacgtgtt
    24301 atttcttggg tagacgtttc atatacagaa aatctaacta atattgatgc taaaattaaa
    24361 agtgctcaag atgaatttta tcaccagttg tcacaagctt tattaaacaa cataaatgag
    24421 atttttggta tacaagaaac aaaaaatatg ttagatcagt ttgaaaatcg gtatcctgat
    24481 ctattaaagg aagtcttccg acatgtgact atacagagaa tttctgaggt attacaaaga
    24541 ttgcttggag aaaatatttc tgttcgcaat ttaaaactta ttatggagtc tttggcgctt
    24601 tgggctccca gagaaaaaga tgtcataaca ttagttgaac atgtccgtgc atcactttct
    24661 aggtatattt gtagtaaaat agctgtttct ggtgagatta aagttgtgat gctttccgga
    24721 tatattgagg atgcaataag aaaagggata aggcaaacct ctggtggctc tttcttgaat
    24781 atggatatag aggtttcgga tgaggtaatg gaaactttag cacatgcttt gagagaattg
    24841 agaaatgcaa aaaaaaattt cgttcttttg gtatcagtag atatacgtag gtttgttaaa
    24901 agacttatag ataacagatt taagagtata ctcgttatat cgtatgctga gattgatgaa
    24961 gcatatacca ttaatgtatt aaagactatt tagtgaggtt taaatatgag taacattaat
    25021 ttagttcaat tagttagaga tagtcttttc acgattggtt gtcctccatc aattattaca
    25081 gatttagaca gccattcagc gattaccatt tctttggata gtatgcctgc tattaatata
    25141 gctcttgtca atgagcaggt tatgttatgg gctaattttg atgcaccaag tgatgtaaaa
    25201 cttcagtcat ctgcttataa catattaaat ttaatgttaa tgaactttag ttatagcatt
    25261 aatgagctgg tagagcttca tcgttctgat gagtacttac agttaagagt tgttattaaa
    25321 gatgactacg ttcacgatgg gattgttttt gcagagattc tgcatgagtt ttatcagagg
    25381 atggaaatct taaatgaggt cttataatca atgagctata caaaattgct cactcaatta
    25441 tcttttccta atagaatctc ggggccaatc ttggaaacaa gtcttagcga tgtttcgatt
    25501 ggtgagattt gtaacattca ggctggaatt gaaagtaatg aaattgttgc aagagctcag
    25561 gttgtaggat ttcatgatga aaaaacaata ttaagcttga ttggaaattc tcgtggactt
    25621 tcacggcaaa cgttgattaa gcccactgcc cagtttcttc atacgcaagt cggccgtgga
    25681 ttattgggag cagtagtcaa tcctttaggg gaggttactg ataagtttgc tgttacggat
    25741 aacagtgaaa ttctttatcg acctgtagat aatgctcctc cgctatatag tgaaagggct
    25801 gcaattgaga agcctttttt aacaggtatt aaggttattg attctttact cacgtgtggt
    25861 gaaggacagc gaatggggat ttttgcgtca gctggttgtg gcaaaacttt tctcatgaat
    25921 atgctcattg aacatagtgg tgctgatata tatgttattg ggttaattgg tgagcgaggt
    25981 cgagaggtta cagaaacggt tgattatttg aaaaactctg agaaaaaaag caggtgtgtt
    26041 ttagtatatg caacttcgga ttactcttcg gttgatcgtt gtaatgctgc atatatagcc
    26101 actgctatag ccgaattttt taggactgaa ggacataaag tagcgctttt tattgattca
    26161 ttaacaaggt atgccagagc attacgtgat gtggccttag ccgctggaga atcacctgcc
    26221 agaagaggct atccggtttc ggtttttgat agcttaccca gacttcttga aaggccagga
    26281 aagttaaagg caggtggctc tattactgca ttttacactg ttcttttgga ggatgatgat
    26341 tttgctgatc cattagctga agaggtaaga tccattttag atggacatat atatttgagc
    26401 agaaatctag cccaaaaagg acaatttcct gcaattgatt ccttaaaaag tataagcagg
    26461 gtatttacac aggttgttga tgaaaaacat cgtattatgg ccgctgcatt tcgggagcta
    26521 ctttctgaaa tagaagagct aaggacaatt attgactttg gtgaatacaa accgggggag
    26581 aatgcctctc aggataaaat ctacaacaaa atatctgttg ttgagagttt tctgaagcaa
    26641 gattatcgac tcggttttac ttatgagcag acaatggagc ttattggtga aacaattaga
    26701 taaggtttta aaaatcaaag ataaatatca acggtctgtt aaattaattg aagcacacat
    26761 attaacacta aaaaaaaaac tctttgtatc gagatgtgga ggcattagat aaaaggatta
    26821 tatacttcct gcaactggag aatgacttag aacctgtagg cgctcagagc gttagtcagc
    26881 ttttcaatac aagacgtaag atcgctatag taaaaaagca tattatccaa tatcagtctg
    26941 aacgaattct tctaaaggga agaattgaag aaattcaaaa agatattgat gaggcaaatg
    27001 cttctaaaag aaaactgctt cataaagaga gtaaaatatg taaacggatt ggcttgatta
    27061 aaagaaataa ctttgccaaa cagttgatac ttgatgagtt atcacaagag gatatgaagt
    27121 atggcattag acaatataaa cctaaatttt agtagtgaca aacagataga aaaatgtgag
    27181 aaactatctt caatagataa tattgatagt ctcgttttga agaagaagag gaaggttgaa
    27241 attcctgagt actctttaat tgcagctaac tattttacta ttgataagca ctttgaacat
    27301 aagcatgata aaggagaaat ttatagtggc attaaaaatg cgttcgaact tagaaacgaa
    27361 cgagcgacat attctgatat tccagaatca atggccatta aagaaaatat tttgatacca
    27421 gatcaagata tcaaagcaag ggaaaaaata aatatcggcg atatgagggg gatcttttca
    27481 tataataaga gtggaaatgc agacaagaac ttcgaaagaa gtcatacttc ttctgtaaac
    27541 cctgataatc tgctagaatc tgataataga aatggtcaaa ttggtttaaa aaatcatagc
    27601 ttgtctattg ataagaatat tgctgacatc atttctttac taaatggaag tgttgctaaa
    27661 tcatttgagc tccctgtaat gaataaaaat actgcagaca taaccccatc catgtcattg
    27721 caagaaaaat caatagttga aaatgataaa aatgtttttc aaaaaaatag tgaaatgact
    27781 taccacttta aacagtgggg ggctggacat tctgttagta tttcagtgga gtctggttct
    27841 tttgttctaa aaccgtcaga tcaatttgta ggaaataaac ttgacttaat tttgaaacaa
    27901 gatgctgagg gtaattacag atttgatagc agtcaacata ataaggggaa taaaaataat
    27961 agtacaggat ataatgaaca gagtgaagaa gaatgctaag aattaaacat tttgacgcta
    28021 acgaaaaact acagatttta tatgcaaagc aactctgcga gcgtttttcg attcagacat
    28081 tcaaaaataa atttacaggc agtgaaagtt tagtcactct tacttccgtg tgtggggatt
    28141 gggtaattcg tattgataca ttatcttttt tgaaaaaaaa atacgaggta ttttcaggat
    28201 tttctacaca agaatcttta ctgcatttat caaaatgtgt ctttatagag tcgtcatctg
    28261 tattttcgat tccagaactg tctgataaga ttactttccg gatcacgaat gaaatccagt
    28321 atgcaactac tgggagtcat ttatgctgtt tttcctcttc tttaggtatt atttattttg
    28381 acaagatgcc ggtattacgt aatcaagttt ctcttgactt attgcatcat cttttagagt
    28441 tttgcttagg ttcatctaat gtaaggctgg ctactttaaa aagaattcgc actggtgata
    28501 taatcatagt tcagaaactt tataatttat tattgtgtaa tcaagttatt attggggatt
    28561 atattgtgaa tgataataat gaggcaaaaa ttaatctgtc agaaagtaat ggtgagtcag
    28621 aacacacaga agtttctttg gcattattca attatgatga tatcaatgta aaagtggact
    28681 ttattctttt agaaaaaaat atgacaatca atgaactaaa aatgtatgta gaaaacgaat
    28741 tattcaagtt tcccgatgac atagttaaac atgtaaatat taaagtaaat ggttctttgg
    28801 ttgggcatgg ggaacttgtt tctattgagg atggttatgg tatcgagatt agttcttgga
    28861 tggtaaagga gtaatctcat gctgagtgac atgtccctca tagctacact ttcttttttt
    28921 acgctattac catttttagt tgctgctggt acctgttata taaagttttc tattgttttt
    28981 gtaatggtac gaaatgcact tggtttgcag caggttccat caaatatgac tctcaatggt
    29041 attgcattga ttatggcttt gttcgtgatg aaacccatta ttgaagctgg ttatgaaaat
    29101 tatctaaatg gccctcaaaa atttgataca atatctgata ttgtccgttt ttctgattct
    29161 ggcttgatgg aatataagca atacttgaaa aagcatacag atcttgagtt agcacgtttt
    29221 ttccaaaggt ctgaagaaga aaatgctgat ttaaagagtg cagagaataa tgattattca
    29281 ttgttttccc tcttgcctgc atatgcgcta agtgaaatca aagatgcgtt caaaataggt
    29341 ttttatttat atttgccatt tgttgttgtt gatcttgtta tttcaagcat tttacttgca
    29401 cttggtatga tgatgatgag tccaatcaca atatcagtac caataaaact tgtattattt
    29461 gttgcgcttg atgggtgggg tatcctgtct aaggccttga ttgaacaata tatcaatatt
    29521 cctgcttaga tgatggaggt aatgtgtctg atatagttta tatgggtaat aaggctcttt
    29581 atttaatcct tatcttttcc ttatggccag taggtatagc tacggttatt ggattatcta
    29641 ttggtttgtt acagacagtg actcaactcc aagagcagac acttcctttt ggtataaagc
    29701 ttataggtgt ctcaatatct ttgctacttc tttctggatg gtatggtgag gttttattgt
    29761 ctttttgtca tgaaataatg tttttaatta agagtggggt ttgatggaca tttcaagctg
    29821 gttcgaaagt attcatgtgt ttttaatact cctgaacggc gtttttttta gattggctcc
    29881 attatttttc tttcttccat ttttaaataa cggtataatt tctccatcta ttagaatacc
    29941 tgtgattttt cttgttgcat caggattaat tacttctggt aaggtagaca taggttcttc
    30001 tgtttttgaa catgtttatt tccttatgtt caaggaaata attgttggcc tccttctctc
    30061 tttttgcttg tctcttccct tttggatatt tcatgctgtt ggtagcatta ttgacaacca
    30121 gcgtggggca acgcttagta gttcaattga tcctgccaat ggtgttgata cgtctgagtt
    30181 ggcaaaattt ttcaatcttt tttctgcagt tgtatttcta tacagtggtg gtatggtctt
    30241 tattttagaa tccatacaat tgtcttataa tatatgcccg ttattttctc aatgttcttt
    30301 ccgtgtctca aatatcttaa catttctgac tttattggca agtcaggctg ttattttagc
    30361 cagtcctgtt atgatagtat tgttactatc agaagtatta cttggtgtat tatcgagatt
    30421 tgctccgcag atgaatgctt tttccgtatc attaactatt aaaagtttac ttgcaatatt
    30481 tattattttc atctgttctt ctactattta cttttctaaa gttcaatttt tcctcggtga
    30541 acataaattt ttcacaaatc tatttgttag ataaaatatt atggcaaata aaacagaaaa
    30601 gccgacacct aaaaaactaa aggatgccgc aaaaaaagga cagtcattta aatttaagga
    30661 tttaacgact gttgttatta ttctggtagg gacatttact ataatatcat tcttttcctt
    30721 aagtgatgta atgctcttat acagatatgt aataattaat gacttcgaaa ttaatgaggg
    30781 taaatacttt tttgcagtgg ttattgtctt ttttaagata attggcttcc cacttttttt
    30841 ctgtgttctt tcggctgtgt tgccaacatt ggttcaaaca aagtttgttc ttgcgactaa
    30901 agctatcaag attgattttt cagtattaaa ccctgttaaa gggttaaaaa aaatatttag
    30961 tataaagaca ataaaagaat ttttcaaaag cattctgctt cttattattc tagcattaac
    31021 aacctatttc ttttggatta atgaccgaaa aataattttt tctcaggtgt tttctagtgt
    31081 tgatggctta tatcttattt gggggaggct gtttaaggat ataatattat ttttcttggc
    31141 attttctgtt tttgttatta ttcttgactt tgtgattgag ttcattttat acatgaaaga
    31201 tatgatgatg gataaacagg agataaaaag agaatatata gagcaagagg gacactttga
    31261 gacaaagtcg agaaggcgtg agttgcatat cgagattctt tcagagcaga ctaaatctga
    31321 tatacgtaat tcaaaattag tggtaatgaa cccgactcat attgcaattg gtatttattt
    31381 taatccagaa atagcgcctg caccttttat ttctctcatt gaaactaacc agtgtgcctt
    31441 ggctgtcaga aaatatgcaa atgaagttgg tataccgact gtgcgtgatg tgaaattagc
    31501 tagaaaacta tataaaacac atacaaaata tagttttgtt gattttgaac acttggatga
    31561 agtcctacgt cttattgttt ggcttgagca ggttgaaaac actcattaat aagtaaagga
    31621 gatgtgttat atgggagtta atttctgtaa taaaataggt attgatcagt ctgaatttga
    31681 aatagaatct tccatcatta actccattgc taatgaggta ttgaacccaa tatctttcct
    31741 tagcaataag gatataataa atgttttact cagaaagatt tcatctgaat gtgacctggt
    31801 tagaaaagac atttatcgtt gtgctctgga gttggtcgtt gaaaaaacac ctgatgattt
    31861 ataatcattc ataataactc ttttgctgat ataaatattt ttgttgaaga tatttatgta
    31921 gtcacaggat caattgtaga aaattgttat gtttttgtta ggaactgcac taaataatat
    31981 atgataagac agcaaaaacg actgaccata atccttcttt tattaggagt tgataagaga
    32041 gactattcat cctgtaatgt taaaactctg ttatatagta ttcgcgatta tgcaaagtct
    32101 gtaaatgatc atgagatact aaccgagagt aatcgtttat tatctcattg catttcagat
    32161 agtaacggag ctttctttaa aagtagtaaa tacgttcccc ttaagtactt acgtaaaagg
    32221 agaatagcac gaaagatacc aaatgattag cactttgcta gtaagcttgc tggtaaacta
    32281 tattgaccgt tgggtttgtt ttgagactgt aatttaaatt gtgtaattgc ctgtttttga
    32341 tatgtttact ccaacaacgg agacaggtaa attatggacg aaaagaagct taaagccctt
    32401 gcggctgaac tggctaaagg cctcaaaact gaggccg
//
LOCUS       DADMGC010000034        32329 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.034, whole genome shotgun sequence.
ACCESSION   DADMGC010000034 DADMGC010000000
VERSION     DADMGC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.034"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(90..626)
                     /gene="ssb1"
                     /locus_tag="JNZ73_001552"
     CDS             complement(90..626)
                     /gene="ssb1"
                     /locus_tag="JNZ73_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HAY8731876.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNKFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            880..3702
                     /gene="uvrA"
                     /locus_tag="JNZ73_001553"
     CDS             880..3702
                     /gene="uvrA"
                     /locus_tag="JNZ73_001553"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAY8731877.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3737..4093)
                     /locus_tag="JNZ73_001554"
     CDS             complement(3737..4093)
                     /locus_tag="JNZ73_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY8731878.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4097..4513)
                     /locus_tag="JNZ73_001555"
     CDS             complement(4097..4513)
                     /locus_tag="JNZ73_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAY8731879.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4624..5337)
                     /locus_tag="JNZ73_001556"
     CDS             complement(4624..5337)
                     /locus_tag="JNZ73_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B acid phosphatase"
                     /protein_id="HAY8731880.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5700..6242
                     /locus_tag="JNZ73_001557"
     CDS             5700..6242
                     /locus_tag="JNZ73_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005059113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731881.1"
                     /translation="MVINFITRDGDDDMNISYVNSNKTTSLPVELDALNNKDISYAKD
                     FSYAKDFFLYIETQLKIAKDFCRPGEEVSSSIASKVFHAFIDLVNKIRGKKDFMYICT
                     LCCFAEEVKGDYSHYRTFLFDIGNQYKVKLIQSGKKEFSLTLEFNDTIIESQKVTGNK
                     AKHILEDIEIFYRNKPDTYY"
     gene            complement(6465..7658)
                     /gene="tyrB"
                     /locus_tag="JNZ73_001558"
     CDS             complement(6465..7658)
                     /gene="tyrB"
                     /locus_tag="JNZ73_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HAY8731882.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7911..8990)
                     /gene="alr"
                     /locus_tag="JNZ73_001559"
     CDS             complement(7911..8990)
                     /gene="alr"
                     /locus_tag="JNZ73_001559"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAY8731883.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9043..10458)
                     /locus_tag="JNZ73_001560"
     CDS             complement(9043..10458)
                     /locus_tag="JNZ73_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HAY8731884.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10541..11524
                     /locus_tag="JNZ73_001561"
     CDS             10541..11524
                     /locus_tag="JNZ73_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HAY8731885.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQPAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11690..11932)
                     /gene="pspG"
                     /locus_tag="JNZ73_001562"
     CDS             complement(11690..11932)
                     /gene="pspG"
                     /locus_tag="JNZ73_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HAY8731886.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12066..13103)
                     /gene="dusA"
                     /locus_tag="JNZ73_001563"
     CDS             complement(12066..13103)
                     /gene="dusA"
                     /locus_tag="JNZ73_001563"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HAY8731887.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13466..14173)
                     /locus_tag="JNZ73_001564"
     CDS             complement(13466..14173)
                     /locus_tag="JNZ73_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HAY8731888.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(14197..14451)
                     /locus_tag="JNZ73_001565"
     CDS             complement(14197..14451)
                     /locus_tag="JNZ73_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731889.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            14769..15284
                     /gene="zur"
                     /locus_tag="JNZ73_001566"
     CDS             14769..15284
                     /gene="zur"
                     /locus_tag="JNZ73_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HAY8731890.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15326..15535)
                     /locus_tag="JNZ73_001567"
     CDS             complement(15326..15535)
                     /locus_tag="JNZ73_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAY8731891.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15651..17030)
                     /gene="dinF"
                     /locus_tag="JNZ73_001568"
     CDS             complement(15651..17030)
                     /gene="dinF"
                     /locus_tag="JNZ73_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HAY8731892.1"
                     /translation="MPPGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHW
                     RNGTWFAAT"
     gene            complement(17049..17657)
                     /gene="lexA"
                     /locus_tag="JNZ73_001569"
     CDS             complement(17049..17657)
                     /gene="lexA"
                     /locus_tag="JNZ73_001569"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HAY8731893.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17767..18135)
                     /locus_tag="JNZ73_001570"
     CDS             complement(17767..18135)
                     /locus_tag="JNZ73_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAY8731894.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18306..20729
                     /gene="plsB"
                     /locus_tag="JNZ73_001571"
     CDS             18306..20729
                     /gene="plsB"
                     /locus_tag="JNZ73_001571"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HAY8731895.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20884..21756)
                     /gene="ubiA"
                     /locus_tag="JNZ73_001572"
     CDS             complement(20884..21756)
                     /gene="ubiA"
                     /locus_tag="JNZ73_001572"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HAY8731896.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21769..22266)
                     /gene="ubiC"
                     /locus_tag="JNZ73_001573"
     CDS             complement(21769..22266)
                     /gene="ubiC"
                     /locus_tag="JNZ73_001573"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HAY8731897.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLFDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22489..24069)
                     /locus_tag="JNZ73_001574"
                     /pseudo
     CDS             complement(22489..24069)
                     /locus_tag="JNZ73_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24298..25218)
                     /gene="malM"
                     /locus_tag="JNZ73_001575"
     CDS             complement(24298..25218)
                     /gene="malM"
                     /locus_tag="JNZ73_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HAY8731898.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLSVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLIIDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTMEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25370..26710)
                     /gene="lamB"
                     /locus_tag="JNZ73_001576"
     CDS             complement(25370..26710)
                     /gene="lamB"
                     /locus_tag="JNZ73_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HAY8731899.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26782..27897)
                     /gene="malK"
                     /locus_tag="JNZ73_001577"
     CDS             complement(26782..27897)
                     /gene="malK"
                     /locus_tag="JNZ73_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HAY8731900.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28262..29452
                     /gene="malE"
                     /locus_tag="JNZ73_001578"
     CDS             28262..29452
                     /gene="malE"
                     /locus_tag="JNZ73_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HAY8731901.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29606..31150
                     /gene="malF"
                     /locus_tag="JNZ73_001579"
     CDS             29606..31150
                     /gene="malF"
                     /locus_tag="JNZ73_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HAY8731902.1"
                     /translation="MDVIKKKHWWQSDALKWSVPGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITTILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31165..32055
                     /gene="malG"
                     /locus_tag="JNZ73_001580"
     CDS             31165..32055
                     /gene="malG"
                     /locus_tag="JNZ73_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HAY8731903.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8228 a         8292 c         7805 g         8004 t
ORIGIN      
        1 gcctccataa cggaggcttc ttttttatgg ctggcagatg ctttaatcat ccaccttaaa
       61 acaatataac ctattgtttt aatgacaaat cagaacggaa tgtcatcatc aaagtccatc
      121 ggcggctcgt tagacggcgc tgccggagcg gactgctgcg ggcgagactg cgcgccgccg
      181 ctgaacttat tgccaccctg cggctgctga ggctgacccc aaccgccctg cggctgacca
      241 ccaccgatat tgccacctgc cggagcgcca ccaccctgac gaccacccag catctgcatg
      301 gtgccgccaa cgttcaccac gacttctgtg gtgtagcgat cctgaccgga ttgatcggtc
      361 catttacggg tacgcagctg accttcgata taaacctgag aacctttacg cagatattcg
      421 ctcgccactt ctgccagttt gccgaacagc acaacgcggt gccattcagt ctgttctttc
      481 atctcgccgg tcgctttatc acgccaggat tcggaagtag ccagcgtaat gttggcaact
      541 gcgccaccat ttggcatgta gcgtacttcc gggtcctgac ccagattacc aacgagaata
      601 accttgttta cgcctctgct ggccatgttc gtgtctcctg aaaaaaatcg ttctgaataa
      661 gtgtaaacgc gcgattgtac cattaccaat agcgctttta ctatgttgtg acctcggttc
      721 cgggaaacaa acctggccag acattgttac acaacactcc ggataatgca ttccaatact
      781 gtatattcat tcaggtcaat ttgtgtcata attaaccgtt tgtgatcgcc ggtagcacca
      841 tgccaccggg caaaaaagcg tttaatccgg gaaaggtgaa tggataagat cgaagttcgg
      901 ggcgcccgca cccataatct caaaaacatc aacctcgtta tcccccgcga caagctcatt
      961 gtcgtgaccg ggctttcggg ttctggcaaa tcctcgctcg ctttcgacac cttatatgcc
     1021 gaagggcagc gccgttacgt tgaatccctt tccgcctacg cgcggcagtt tctgtcactg
     1081 atggaaaagc cggacgtcga tcatattgag gggctttctc ctgccatctc aattgagcag
     1141 aaatcgacgt ctcataaccc gcgttctacg gtggggacaa tcaccgaaat ccacgactat
     1201 ttgcgtttgt tgttcgcccg cgtcggcgaa ccgcgctgtc cggaccacga cgtcccgctg
     1261 gcggcgcaaa ccgtcagcca gatggtggat aacgtgctgt cgcagccgga aggcaagcgt
     1321 ctgatgctgc tcgcgccaat cattaaagag cgcaaaggcg aacacaccaa aacgctggag
     1381 aacctggcaa gccagggtta catccgtgct cgtattgatg gcgaagtctg cgatctttcc
     1441 gatccgccga aactggaact gcaaaagaaa cataccattg aagtggtggt tgatcgcttc
     1501 aaggtgcgtg acgatcttac ccaacgtctt gcggagtcgt ttgaaaccgc gctggaactt
     1561 tccggtggta cagcggtagt ggcggatatg gacgacccga aagcggaaga gctgctgttc
     1621 tccgccaact tcgcctgccc aatttgcggc tacagtatgc gtgaactgga gccgcgactg
     1681 ttttcgttta acaacccggc aggtgcctgc ccgacctgtg acggccttgg cgtacagcaa
     1741 tatttcgatc ctgaccgcgt gatccaaaac cccgagctgt cactggctgg cggtgcgatc
     1801 cgtggctggg atcgccgcaa cttctattac ttccagatgc tgaaatcgct ggcagatcac
     1861 tataagttcg acgtcgaagc gccgtggggc agcctgagcg cgaacgtgca taaagtggtg
     1921 ttgtacggtt ctggcaaaga aaacattgaa ttcaaataca tgaacgatcg tggcgatacc
     1981 tccatccgtc gtcatccgtt cgaaggcgtg ctgcacaata tggagcgccg ttataaagag
     2041 acagaatcca gtgcggtacg tgaagaatta gccaagttta tcagcaatcg cccatgcgcc
     2101 agctgcgaag ggacgcgtct gcgtcgggaa gcgcgccacg tttatgtcga gaatacgccg
     2161 ctgcctgcta tctccgacat gagcatcggt catgcgatgg aattcttcaa caatctcaaa
     2221 ctcgctggtc agcgggcgaa gattgcggaa aaaattctta aagagatcgg cgatcgcctg
     2281 aaattcctcg taaacgtcgg cctgaattac ctgacacttt cccgctcggc agaaacactt
     2341 tccggcggtg aagcccagcg tatccgtctg gcgagccaga ttggtgcggg cctggttggc
     2401 gttatgtacg tgctggacga gccgtctatc ggcctgcacc agcgcgataa cgagcgcctg
     2461 ttgggtacgc ttatccatct gcgcgatctc ggtaataccg tgattgtggt ggagcatgac
     2521 gaagacgcaa ttcgcgccgc tgaccatgtg atcgatatcg gtcctggtgc gggtgttcac
     2581 ggcggtgaag tggtcgcaga aggtccgctg gaagcgatta tggcggtgcc tgaatcgttg
     2641 accgggcagt acatgagcgg taaacgcaag attgaagtgc cgaagaaacg cgttccggcg
     2701 aatccagaaa aagtgctgaa gctgacaggc gcacgcggca acaacctgaa ggacgtgacg
     2761 ctcacgctgc cagtcggtct gtttacctgc atcacagggg tttcaggttc cggtaaatcg
     2821 acgctgatta acgacacact gttcccgatt gcccaacgcc agttgaatgg tgcgaccatc
     2881 gccgaaccgg caccgtatcg cgatattcag ggactggagc atttcgataa agtgatcgat
     2941 atcgaccaaa gcccaattgg tcgtactcca cgttctaacc cggcgaccta taccggcgtg
     3001 tttacgcctg tgcgcgaact gtttgcgggc gtaccggaat cccgtgcgcg tggttatacg
     3061 ccaggacgtt tcagctttaa cgtccgtggc gggcgctgcg aagcctgtca gggcgacggt
     3121 gtgatcaaag tggagatgca cttcctgccg gatatctacg tgccgtgcga tcagtgcaaa
     3181 ggtaaacgct ataaccgtga aacgctggaa attaagtaca aaggcaaaac catccacgaa
     3241 gtgctggata tgaccatcga agaggcgcgt gagttctttg atgcggtgcc agctctggcg
     3301 cgtaagctgc aaacgttgat ggacgttggt ctgacgtaca ttcgcctggg gcagtccgca
     3361 accacacttt ctggtggtga agcccagcgc gtgaagctgg cgcgtgagct gtcaaaacgc
     3421 ggcaccgggc agacgctgta tattctcgac gagccgacca ccggtctgca cttcgccgat
     3481 attcagcaac tcctcgacgt gctgcataaa ctgcgcgatc agggcaatac cattgtggtg
     3541 attgagcaca atctcgacgt gatcaaaacc gctgactgga ttgtcgacct gggaccagaa
     3601 ggcggcagtg gcggcggcga aatcctcgtc tccggtacgc cagaaaccgt cgcggagtgc
     3661 gaagcttcgc atacggcacg cttcctcaag ccgatgctgt aatcgttaag gccgctttct
     3721 gagcggcctt ttcctttcag agttgtacca gcaatttacg cttttcttcc ggtagtaaat
     3781 tcactgcctg ctgataagac gcatccacca gataatagat ttgcgaatcc ggcagcgaac
     3841 cgtcgagata cacggtgctc cagtgcgctt tattcagatg gcggctagga cgcacatcgc
     3901 tgtgctgctg acgtagcagc tccgccagct ccgggctggt tttcagcgaa acagctgggc
     3961 gattttctac ttctttcacc atcgcaaaca gtacatcttc cactttgatc tgcgtcgctt
     4021 tccagtcgtt atgcacgctc tgttctgcgc ctggttttgc catgcaatat tgtagcaact
     4081 ccgaaatggt cattttttac tccccttgta gtgtcgcgat gatgcgacgc gatccgccgt
     4141 ggatgcgatg ttcccccagc cagatgcctt gccaagtgcc ggtctgaata cgccctttat
     4201 gtaccggcaa tacaagcgat gttcccagca ttgaggattt gatatgagaa ggcatatcgt
     4261 ctgctccctc atagtcatgc tcataatttc cgttgtcggg aacggtgcgg aggaaaaaac
     4321 actccatgtc gtggcgtacg gtgggatcgc agttctcatt aagtgtcaga gaggcggagg
     4381 tatgttgcag caacagatgc agtaagccga tgttaacgcg cggcatatca gccagctgat
     4441 tcagaatttc atccgttacc agatgaaacc cacgagattt ggcgctaagc gtgagcgtct
     4501 tttgatacca catgtgctgc tccttgataa aactctctta atcagtttgc agcaagacag
     4561 cgaaaggata aaggtgtgat taaaaaaaca gcattcggga gagcatcact ctctcccgct
     4621 ctgtcagtat tctgaattga cgatcacctc ttcaccaaac gcacccgctt gtggcaaggg
     4681 tttgtaggta gagttggagg cgcgcagaat gcggatacca cgagcgccga catcgcgtgc
     4741 ggcggtaata tcgttatcgg aatcgccata aaaaattcgg atatttttat cctgcagcca
     4801 ttgcgatttt gtattttgcc ctggtttatc gcccgcaaag attaccggat tcatgttggt
     4861 ggcaggaata tgaaaattat ccgccagcgt ttttgaaacc gtttctgttt tcgtcgggct
     4921 acgaccagtc acaaagaaga tcgcgtcacc gcggcgtaca tgcatatcaa tcagctggcg
     4981 agcgacctct tttggaatgc tgaattcatc ccagccatta ttcatttttt cccagaacac
     5041 aggatttttc agataatctt cgctttctgg cgagaaggtt tttttgccgc gccagaaccc
     5101 cggactggaa aaaagtaccg tgtcatcgat atcaaacccc accgccattg gcggacgccc
     5161 tgcgaggcta ttttcaattt gtgcgaccga aacccaatga attggtgcct gttcagcaag
     5221 cctggcaacg ttagtcccag ggttaagcgg tgaaggagat gaggccaggg caacagcgga
     5281 actgtttaga gcgaacaata agcaaacggc actgattgcc tgtgtgatct tgcgcatatt
     5341 tttccctaaa tagtcagtat gttgaaactt tttattgtga gatttgttgc aaaaaccatc
     5401 tgaccataac gccagctgcc ggggaaagga aggattatct gcggtttttg tgatgaaaag
     5461 caggatgatt atatttaacc cagtaaatac gttcctgtct ttttttgtat aaaaaatgtt
     5521 tattactcga tgtaaggata cattaatttt acgtttatta tgatgaatcc tcggcaaatg
     5581 agcgaaattt gatagaacat ttatctgctg tacgatttaa tgaacttaat gatacagcat
     5641 tacaaaaaca atcgaagttt ataaagatga tttctgattg accagccccc taccctacaa
     5701 tggtgattaa ttttattact cgcgatggag atgatgatat gaatatatca tatgtgaata
     5761 gcaacaaaac gacatcttta cctgttgagc tagatgcact aaataataaa gatatctctt
     5821 atgccaaaga tttctcttat gccaaagatt tctttttata catagaaaca cagttaaaaa
     5881 ttgcaaaaga tttttgtaga cctggagaag aagtatcaag ttctattgca agtaaagttt
     5941 ttcacgcatt tattgattta gttaacaaaa tcaggggtaa gaaagacttt atgtatattt
     6001 gcacgctttg ctgttttgcc gaggaggtta aaggtgatta ttctcattac aggacctttt
     6061 tatttgatat tggtaatcaa tataaggtta aacttataca aagcggaaaa aaagagttct
     6121 ctttaacatt agaatttaac gatactataa ttgaatctca gaaagtcaca ggcaataagg
     6181 caaagcatat tcttgaggat atagaaatat tctatcgtaa caaacccgat acttattatt
     6241 aaatttaata atggttagat gtgcagaaat tattactcca cacgaaaaaa tagggaaagg
     6301 aattccagat aattgggata aacttaacaa taaaagaact gacaacgttt tattatctct
     6361 cacgtagaac aacggcatca aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt
     6421 ttaatttcac tgcaggctgg gtaactccag cctgctttcc tgcattacat caccgcagca
     6481 aacgcctttg ccacacgttg tacatttgcc gtatttaacc cggcgacaca catgcgaccg
     6541 ctggcgatga gatagacacc aaattcttca cgtagtcggt caacctgagc ggcacttaaa
     6601 ccggtataac tgaacatgcc gcgctgatta agcagataat cgaaattgcg ttctggcatc
     6661 tctgtgctta ataccttcac caattcctga cgcattgcca gaatgcgagt acgcatctct
     6721 tctacttccg ccagccagct ggctttcaat gcctcgtcat tcagcactgc agccaccacc
     6781 tgcgcaccaa aattcggcgg gctggagtag ttgcggcgaa ctgttgcttt caattgcccc
     6841 agtacgcggc cagcggcttc ggcatcttca cacagaacag aaagtccgcc gacgcgctcg
     6901 ccgtaaaggg agaaaatttt cgagaacgaa ttgctcacca gagcgggtaa tccagcgctg
     6961 gcaatggcgc gaatagcgta ggcatcctct tccataccgg cactaaatcc ttgataggca
     7021 atatcgagga atgggataag ctcgcgggct ttgagaattt caatcaccgc atcccactga
     7081 tcattagtga gatcggcacc cgttgggttg tggcaacatg gatgcagcaa cacaatactg
     7141 cgggcaggta atgttttcag cgtcgccaac aggtcattaa agcgcacgct gttagtcact
     7201 tcgtcatacc aggggtaagt actcacttcg aatccagccc cggcgaatat tgctacgtgg
     7261 ttttcccaag taggatcgct gacccagacg cctgattccg ggaagtagcg tttcaggaaa
     7321 tccgcgccca ctttcaatgc ccctgagccg ccaagggttt gaatggttgc tacgcgctgt
     7381 tgttgcagta ccggatggtc cgcaccaaac agcagcggcg caatggcatg gcgatagctg
     7441 ttaagccctt ccatcggtaa ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct
     7501 tccgcctccg ccacggcttt caattgtgga ataattccgt cttcgttgta gtacagaccg
     7561 atacttaaat tcactttgtc gctgcgaggg tcttctttaa aacgctccat aagcgtaaga
     7621 atcgggtcgc cagcgtaggc gtcaactttt tgaaacacgc gatggttctc caggtttacg
     7681 ggcaggtggt taaaacacaa taaaccggaa gaaggcgaag atcgagtgga tgttcaggag
     7741 cgaacggcaa ttagcaacag agtgagacta atgataaacg tacatccgcc agaggcacga
     7801 cctttataag agcatgaaag aacatcaact tattgaattt ttaggatttt attggccgga
     7861 taaggcattc acgccgcatc cggcacagac aatcaaatat tacagaacga ttaatccacg
     7921 tatttcatcg cgacccttga agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc
     7981 atttcagcga tacgttctac gggcaaacct tcgccccata aaatgaccgg atccccggct
     8041 ttgtcctgcg cctgtggacc taagtctacg cagatcatat ccatcgccac gcgcccgaca
     8101 atcggtactt cgcgaccgtt caccagcact ggcgtaccgg acggcgcggc gcgcggataa
     8161 ccatcgccat agcccatcgc gactacgcca agacgggtat cacgttcgct tacccaggtt
     8221 ccaccataac caacaggctc tccggcttta tgctcacgca cggcaatcag gctggaggtt
     8281 agtgacatca ctggctgaca gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag
     8341 acgccataaa gaatgatgcc cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga
     8401 atgccacccg acgcggcaat ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag
     8461 atagcgagtt gtttctcggt tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg
     8521 acgatattca ccggctgacg aacgtttttg cactgggtca ggcgatgata aaacgcctca
     8581 gcctgttccg gccttacgcc cagacggtgc ataccggtat cgagtttcat ccagacggta
     8641 accggctcgt ccaagctagc ctcttccagc gcagccagct gttcttcgtt atgcacggcg
     8701 gtatgaaaat gttgcgcaga aatcgtcggc agatctctgg catcaaaaaa gccttcgagt
     8761 aacagtacag gtttggtgat tccccccgca cgcagtcgca gagcttcttc gagacgggct
     8821 acgccaaagg cgtcagcatc ggggagcgtt cgcgcggtct caagaagacc gtgaccataa
     8881 gcgttcgctt tcaccaccgc aaccatttta ctggcaggtg ccagttcacg aagacgttgc
     8941 aggttgtgtc gcagagcgcg gcggttaatc acaacagttg ccgcttgcat ttgtgttcct
     9001 tgataattgt ttgctttaat tacctaattc ataaaataat tattattcgt cgtcgtactg
     9061 cggccccgca tagttgtcga agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc
     9121 gattgggccg ttacgttgtt taccgataat aatttccgcg atgcctttta aatcactgtt
     9181 ttcgtgatac acctcatcac gatagataaa catgatcaag tccgcatcct gctcgataga
     9241 gccagattca cgcaggtcgg agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg
     9301 gttcaactgg gacagcgcca ccaccggcac gttcagttct ttcgccagtg ctttcagcga
     9361 gcgggagatt tctgcaattt ccagcgtacg gttatcggaa agcgccggta cgcgcatcag
     9421 ttgcaggtag tcgatcatga taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc
     9481 gcgggaacgc acttccgttg gcgtcaggcc ggaagagtca tcgatataga tattgcgttt
     9541 ttcgagcaaa atacccatgg tgccggaaat gcgcgcccag tcttcatcat cgagctgccc
     9601 ggtacggatt ttagtctggt caacgcgcga cagcgacgcc agagaacgca tcatgatctg
     9661 ttctgatggc atctccagcg agaagataag tactggttta tcctgcaaca ttgccgcgtt
     9721 ttcgacgagg ttcatcgcaa atgttgtttt acccatcgac ggacgcgcgg cgacgatgat
     9781 caaatccgac ggctgcaagc cagcggtttt tttgttgaga tcgtcataac cggtgtttac
     9841 cccggtaacg ccatcgtgtg gctgctgaaa caactgctca atacgcgcca cggttgcgtc
     9901 gagcacatcg gcgatgttct tcggcccttc gtctttgttt gcacgacttt cggcaatttt
     9961 aaagacgcgg gattcagcaa ggtccagcag atcttcgctg gtacgcccct gcggatcaaa
    10021 accggcttca gcaatctcat tcgcaaccga gatcatctca cggacaacgg cacgttcacg
    10081 cacgatgtcc gcataagcac tgatgttcgc cgcacttggc gtattttttg acagctctgc
    10141 cagataagca aaaccaccga cgctatcgag ttgcccctgg cgttccaacg attccgcaag
    10201 ggtaatcaga tcgataggac taccgctttc ctgcaaacgc gccatttcag taaagatatg
    10261 acggtgtggg cgggtgtaaa aatcgtctgc taccacacgc tcggctacat catcccagcg
    10321 ttcgttatcc agcattaaac cgcccaacac cgactgctcc gcttcgatcg agtgcggagg
    10381 cactttcagc ccggcaactt gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa
    10441 gggtttattt cctgccatag tgaatggagt taccgaaata aagaatgggt cgaaacttta
    10501 ccatatgaag cagaccctga cgatacgttc tggaggacac atggcaacac gaattgaatt
    10561 tcacaagcac ggtggcccgg aagtacttca agccgtagag ttcactcctg ccgatccggc
    10621 ggagaatgaa atccaggtcg aaaataaagc catcggcatc aattttatcg acacgtatat
    10681 ccgcagcggc ctttacccgc cgccatcgct acccagcgga ttaggtaccg aagcggcagg
    10741 catcgtgagt aaagtcggca gtggtgtaaa gcatattaag gctggcgatc atgtagtcta
    10801 tgcgcagtcg gcgttaggcg cttacagctc tgtgcataac attaatgcgg ataaagcggc
    10861 gattctgcct gcggcaattt cttttgagca acctgcggca tccttcctga aaggcttaac
    10921 ggtttattat ctgctgcgca aaacctatga aattaaaccc gatgaacagt tcctgttcca
    10981 cgcagcggct ggcggcgttg gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa
    11041 actcatcggc accgtaggaa ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg
    11101 gcaggttatt aactatcgtg aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg
    11161 taagaaagtg cgcgtagtgt acgattccgt gggcagagac acctgggaac ggtcgctgga
    11221 ttgcctgcaa cgccgcggct taatggtcag ttttggcaac tcatcaggtg cggttaccgg
    11281 tgtgaactta ggcattctca atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca
    11341 aggctatatc accacgcggg aggaattaac cgaggccagt aatgaactgt tctctttgat
    11401 tgccagcggt gtgattaagg tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc
    11461 gcagcgtgcg catgagattc tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc
    11521 ataaaagaaa tagggcttcc acctgggaag ccctttcttt ttatagttcg gctgtatgta
    11581 gggtacagca cgatgaatct gttagaggcg caatagtgac agatttgatt atcaattcct
    11641 attttgttct aaggataaaa ccttaggttg tgatcatccg cacaatccct tagtaacgcc
    11701 agcggtcata acgctgatat ttcggcattt ttggtgcttt aatcgcctta ataacccaca
    11761 ccaccgcaat cgccagtagt aaccacggca gcaacttaat catcaatgcc agcataccgc
    11821 cgaggaacat aatggccgtc gccacaacca gcgcggcgat aatgcccagc aacgaaacgc
    11881 cggtgaccat cagcatgaca aaaaagccaa tcacaaaaag tagttccagc atgatgctct
    11941 cccaaatatg aaatctcttg ctggcattac aagaatcatg ccaaaaataa tctattgatt
    12001 taacagcaaa acgccccgcg acggtgcgca gggcgtggtg aatttgacta ctttttggtg
    12061 aaaagttaac gcttatccgc caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca
    12121 cccgctttat gggcattttc acttaaataa cgccgccact gccgcgcgcc aggaataccc
    12181 tggaacaaac ccagcatatg ccggataata tggccgagat acgtcccctg gctgagttca
    12241 tgctcaatgt acggatacat ggcacgcact actgccaccg gatcggcatc ggtatccgag
    12301 gaaccaaaga tctcccggtc taccgccgcc agaatacccg gattctgata cgcctcgcgc
    12361 ccgaccatca cgccatccat atgttgcaaa tgcgctttag cttcttccag cgacttgata
    12421 ccaccgttaa tcgacattgt cagatgcggg aagtcacgct tcagttgata cacacgcgga
    12481 taatcgagcg gcgggatttc acggttttct ttcgggctta accccgaaag ccaggcttta
    12541 cgtgcgtgga tgatgaacat ctcacactcg cctttgccgg aaacggtatt gatgaaatcg
    12601 cagagaaatt catagctgtc ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga
    12661 atcgacacca catcgcgcat cgctttcacg cagtcggcaa ccagctgcgc attacccatc
    12721 agacacgcac caaacatgcc gttctgcacc cggtcagacg ggcagccgac attcaggttg
    12781 atctcatcat atccgcgcgc ttctgccagc tttgcacact gcgccagcgc cgccggatcg
    12841 ctaccgccca gttgcaacgc taccggatgt tcttcttcac tgtacgccag ataatcacct
    12901 ttaccgtgaa taatcgcccc tgtggtcacc atttcggtat aaagcaacgt attgcgggaa
    12961 agcagacgca agaaatagcg gcaatgtctg tccgtccagt cgagcattgg tgcaacacta
    13021 aaccgaccac tccagtgaac gtcagttttt tcaggcattg cgctggtttg gttgattttt
    13081 tgcatttcag aattaccgtg cattttcaaa tgtagagatt attttatcga tatatcatgg
    13141 ggttatgtta ttcagcttca ctgttcagga ggctcaatag cggggtacta taccataaca
    13201 acaggaagcg cctgtctcat tgcaaaagaa aattgagatc ctctcaaggc atgaagctct
    13261 cacgaagtga tggaaataat cttattagcc gttaactttg ttaaggccaa tgataaacaa
    13321 tccaggttcg acgataaata aaaaatcaca cattaaactc tggtgatata tctccctgct
    13381 aatagcattg atagagaaaa aagaacccaa taaatattgg gtccttttat ataatgcctt
    13441 ccatactatt gaagaacttc acatattatt tctccgattt aaccccgaac aagtcataaa
    13501 ttaatttaga agtgtctgta agtattttaa tctcttcctt tgaggttggg tcaaacgact
    13561 tcgcgaagct aattaattgt ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga
    13621 gcgttccttc attcaccaga tgtgacatct tatccagata gccatcaaaa ttcatttctc
    13681 ccccatctgc cagacgcttc atctctccca ggtacttctt catatcacgt tgatataatt
    13741 tttcaaactg agctttcagg tagctctcat tattttcatt aatatacact gtttctgata
    13801 aatctgcgta ggcactagaa tatgaaagtg aaattaacag cgaagcaaat aattttaagc
    13861 tgttgtcatc atgttcctgg caggcattaa caaacgttaa aaaaccagaa cctattgctt
    13921 gaaaatgtat attcgctaaa ggttcattat ccttagattc ctcataaaat agctgaaccg
    13981 tggagggaag gccattctta gtttctacat caaaagtaca ttgttcaatg ggcattgcat
    14041 ctttattatc cccaggatta catatactat taatcgcgaa atgaaaaata gcccggtcaa
    14101 caactgatgc aataaagtca cgatcgttta aatcctcatc agtgcaatcg tcagtatact
    14161 tattgaccca cattttgaga tcatttattt gctcaattaa aaacacattt ttttacgtgc
    14221 atgccataaa ttatgcgtga ttcgttgaat aaacaaatct atttttgcaa taattatatc
    14281 ccgatgacgc agcgctgcat aatcaataga tttaccatat atattattta atttaccttt
    14341 gattgaacct ctattatcca atttatcttt ggttgcatct ttattttcga attgatttga
    14401 attgagtgtt ttattaaagt atccagttgc accgggtatt atcttcaaca tgattcatct
    14461 accttttttg tcgtaccgtg tcctccttat aattcttttg ccatctggtc actatcaaaa
    14521 atcgctcata atttctgaac tacaggtatg cgtttaaacc cagttaaata aatagcgagt
    14581 taacttgaag caatttattt aaaaatgaat tattaattat tatcgtgttg ataatggtac
    14641 gacaaggcaa cacaacacct tacctacaaa acagaaaatc aacattacgg caacaataag
    14701 ggttctcggt gtttttcctg gaacatggta aagtaaggac attcttaacc cccactttga
    14761 ggtgcccgat ggaaaagacc acaacgcagg agttattagc gcaggctgaa aaaatctgcg
    14821 cgcagcgtaa tgtgcgcctg accccacagc gcctggaagt gttgcgcctg atgagtctgc
    14881 aggatggcgc tatcagcgct tatgatctgc ttgatttact gcgcgaagct gaaccgcaag
    14941 ccaagccgcc aacggtttat cgcgcgctgg attttctgct tgagcaaggt tttgtgcata
    15001 aggtggaatc caccaacagt tatgtgctct gtcatctgtt cgaccagccc acgcatacgt
    15061 cagccatgtt tatttgcgat cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg
    15121 aagacattat gcatacgctg gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg
    15181 aagcacatgg gctctgtgcg gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt
    15241 gccagcatga tcactctgtg caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt
    15301 gtacacgtcg ggcaggaggg attaattacc agcgatattc attgcgggtt tcccaatcca
    15361 cgacctcttt ttctgcctga tctttctgat aaccataacg ttcctggatt ttaccgacca
    15421 gttgatcacg tttaccttca atgatcgtca tatcatcatc ggtcagtttg ccccattgct
    15481 ctttcacttt acctttaaac tgtttccagt taccgccggc ttcatcttta ttcataatca
    15541 agacctcatc gttaggttgt gaatgagagt acgttcactt ttcttctgaa cgtgagatta
    15601 agtgtagtca gcaatttggc ttaggataat ttattcagaa tttttaaccg tcacgttgcg
    15661 gcaaaccagg taccgttgcg ccagtgacgc cgccagatag ccgccagaga aagcccgcgc
    15721 aacgccagaa agacggttaa tgccagccac aaaccatgat tacccagcca cggcagcgta
    15781 aggagcgtca gcgcaaaacc tgcggcggcc accgccatac tgttacgcat ttcggcggca
    15841 cgcgttgcgc ctataaacat gccgtccagc agataacacc agacgccaac caacggcaaa
    15901 atcacctgcc agataagata gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc
    15961 aacgcaatga tgtgttcccc tgccagcaaa taaaccaccg aaaacagtaa cgctacaatc
    16021 cccgactggc ggcacgctgc ccgccataca tccagcaact ggctaccgtc gcgcgcaccg
    16081 tatgcctgac cggagtgcgc ttcaaccgcg taggcaaaac catcaagcgc ataggcggta
    16141 aaggtgagta gcgtcatcag aaccgcgtta acagcgataa tgtcactccc cagtcgcgca
    16201 ccaagtacgg tgatcgcgcc gaaacagagt tgcaacaaca gcgaacgcag catgatatcg
    16261 cggttaagcg ccagcaagcg acggaagttt cctcgccagg cagttttcag catttcgccg
    16321 gaaattccgc gtagtttgag gattttacgc accattagca gaccaatcag caatgttgca
    16381 tattccgcaa taaccgtcgc cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc
    16441 agccagacat ccagcacaat gttgaggata ttgccgacca ctaacaaaat tactggcgca
    16501 cgggcatatt gcacgccgag taaccaaccg agtaatacca gattcgccag cgacgccgga
    16561 gcgcttaacc agcggatttc aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg
    16621 ccaacaatat gcagcgccag atcgataatc ggcgtacgca acagcgcaat taacgccccg
    16681 gcccccaacg ccaacagcaa cggttgcacc agcgcacggg ctaatgcctg aggatttttg
    16741 gcaccataag cctgcgcagt cagcccggtg gtgctcatgc gtaaaaacag caacagcata
    16801 aagagaaagc tggtcgccgt tgcaccaacc gccacgccgc ccaaataaac cggactatca
    16861 agatgaccaa ttaccgccgt atcgaccagt cccagcaacg gaacggtgat attggagaaa
    16921 atcatgggta aggcgagatg ccagagtgct ttatcagatg aagtgaggaa tgccatgcag
    16981 acaagcctga tgaagagaga tgaaaaacaa accgcgacgc caggcggcat cgcggtctca
    17041 gagatatgtt acagccagtc gccgttgcga ataaccccaa ccgccagccc ttcaatggtg
    17101 aagctctgct gacgaaggtc aacgacaatt ggtttaaact cgctattttc tggcaacagt
    17161 tcgactttat tgccctgttt tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg
    17221 acaacgacct gaccgttacg tacatcctga gttttatgca ctgccagcaa gtcgccatcc
    17281 ataatgccga tatctttcat cgacatcccg ctgacgcgca gcaggaaatc agcattcggc
    17341 ttgaacaagg aaggatcgac ctgataatga ccttcaatat gctgttgcgc cagaagcggt
    17401 tcaccggcag ccacacgacc taccagcggc aacccttctt cctcttcctg caacagacga
    17461 atcccgcgtg atgcgccgga aacaatttca ataacgcctt tgcgtgccag cgccttcaga
    17521 tgttcttcag ccgcgtttgg ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc
    17581 ggcggcatac ctgtctggct gatgtgatca cggatgagat caaacacctc ttgttgcctg
    17641 gccgttaacg ctttcattcc gccccctggg tgtatataca gttatactgt gagtatatac
    17701 agcaaaaggc gattttggaa ccataaactg cacaataaac cagagattta tcgaaatctg
    17761 gaagggttat ccaaaatgcg accataacag gatgcaccag gtaatcacgg cgacgataat
    17821 ggcaatcagc accgcggcgg atcccatatc ttttgcgcgt ccggaaagct catggtattc
    17881 agagccaatt cggtcaacca ctgcttcgat ggcgctattg aggatttcca caatcatcac
    17941 cagcatcacg gagctgataa gcagcacgcg ggtaattgcg tccacatcca gccagcaggc
    18001 gatgaccacc gccaacaata ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca
    18061 tgcagcgcgt aaacctttcc aggaatagcc agcagctttg ataattcggg tgaatccagt
    18121 ggtattattg gccattgaaa gaaccttttt acattatgag cgtcaatatc agtgtaccgt
    18181 taaatccgat gattcgggtt tgcgtaacgc cgcgcgaaac atgagcggat accacagaat
    18241 ttcccatgac tttctgctat ccttgccgcg catttgcatt attaaccaga ggctttacat
    18301 cgtttatgtc cggctggcca cgaatttact acaaattact gaatttacca ttaagcatcc
    18361 tggtaaaaag caagtctatt ccggcagatc ctgccccgga actggggctg gatacctctc
    18421 gtccaattat gtacgtttta ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc
    18481 agtgtctggc acatgatttg cctgacccgt tagagccgct ggaaatcgac ggcacgctac
    18541 tgccgcgcta tgtgttcatt cacggcgggc cgcgtgtatt cacctattac acgccgaaag
    18601 aagagtctat taagcttttc cacgactatc tcgatttgca ccgtagcaac ccaaatctgg
    18661 atgtgcagat ggtgccagta tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg
    18721 aagtgaaccc gccgctgcgt atgcttaacg gcgtacagaa atttttcgct gtactgtggc
    18781 tcggtcgcga cagttttgtg cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg
    18841 aacacggcac ggataaaact atcgctcaga aactggcgcg cgtggcgcgt atgcactttg
    18901 cccgtcaacg tctggctgcc gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata
    18961 agctgctcgc ctcccgcgcc attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa
    19021 tctcccatga aaaagcgcag cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt
    19081 tctcttacga gatgatccgc ctgactgacc gcattctggg cttcacctgg aaccgatttt
    19141 atcagggcat caacgtccat aacgccgagc gcgttcgcca gctggcccac gacggtcatg
    19201 agctggtgta tgtgccttgc caccgcagtc acatggacta cctgctactt tcttacgtgc
    19261 tgtaccacca ggggctggtg ccgccgcata tcgccgccgg gatcaacctg aacttctggc
    19321 cggccgggcc gattttccgc cgtctggggg cgttctttat tcgccgtact tttaaaggca
    19381 ataaacttta ttccaccgtt ttccgtgagt atctcggcga actgttcagc cgtggttatt
    19441 ccgtcgagta cttcgtggaa ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa
    19501 ccggtacgct gtcgatgacc attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc
    19561 tgattccgat ctatatcggt tatgagcacg tcatggaagt gggtacttac gccaaagaac
    19621 tgcgcggcgc gacgaaagag aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc
    19681 tgcgtaatct cggtcagggt tacgtcaact tcggtgaacc aatgccgttg atgacctacc
    19741 ttaaccagca cgtaccagac tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg
    19801 cctggttaac gccgacggtc aataatattg ctgccgatct gatggtacgc attaacaacg
    19861 caggcgcggc aaacgccatg aacctgtgct gtactgcgct actggcatca cgtcagcgct
    19921 cactcacccg cgagcagtta accgagcaac tcaactgcta cctggatctg atgcgcaacg
    19981 taccttactc cacggactct accgttcctt cagccagcgc cagcgagctt atcgatcacg
    20041 cgctgcaaat gaacaagttt gaagtcgaga aagacactat cggcgacatc atcattctgc
    20101 cacgcgagca agcggtgctg atgacctact atcgcaacaa cattgcgcat atgttggtgc
    20161 tgccttcgct gatggcggca attgtcaccc agcatcgcca catctcccgc gacgtattga
    20221 tggagcacgt caatgtgctt tacccaatgc tgaaagcgga gctgttcctg cgctgggatc
    20281 gcgacgagtt gccggacgtt attgatgcgc tggcaaatga gatgcaacgt caggggctga
    20341 ttaccctgca agatgatgag ttgcatatca acccggcgca ttctcgcacc ctacagctgc
    20401 tggccgcagg cgcgcgcgaa acgctgcaac gttatgccat caccttctgg ttgttgagtg
    20461 ccaacccgtc gatcaaccgc ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc
    20521 tctccgtgct gcacggcatc aacgcgccgg agttcttcga caaggcggtg ttcagttctc
    20581 tggtgctgac gctgcgtgat gaagggtata tcagcgatag cggcgatgcc gaaccagcag
    20641 aaacgatgaa ggtttatcag ttgctggcgg agttgattac atcagacgtg cgtttgacga
    20701 ttgagagtgc gacgcagggc gaagggtaat cagagagaat tgccggatgc ggcgaaaacg
    20761 ccttatccgg cctaccatga cctgcaaatt caataaattg cgattcaccg ggtaggcctg
    20821 agaagcgcag cgcatcaggc aacccagaag aaagccggat gatcatccgg cttttttaca
    20881 tcatcagaaa tgccagtaac tcattgccag tcctaaaaac agtaccagac caacatagtt
    20941 attattcata aatgctttaa agcaggcttc acgctcgcgg ttggcaatca gtttttgttg
    21001 ataaacaaac agcgcgccag ccaccacaat tgaccagtaa tatccccagc ctaagccatt
    21061 taactcaccg atgatcgcca tcagtgccag tacgccaatc tgcaaaatac caataatcaa
    21121 tttatcgtat tggccgaaca ggattgccgt ggatttaatg ccaatcttca catcatcatc
    21181 gcggtcaacc atcgcatact gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa
    21241 cattaaccag caactcaatg gcaccgactc actcacagcg gcaaaagcca ttggaatcga
    21301 ccagccaaac gccgcgccca gcaccacttg cgggagatgg gtataccgct tcataaacgg
    21361 gtacacccac gccaatgcca gagcggcaat cgacaacaga atggtcatcg tattcagcgt
    21421 cagaaccagt aaaaacgaaa tcagtaccag cactacaaac agcgcgcgcg cctctttctc
    21481 tgttaccgcg ccgctgggaa gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt
    21541 gcgatcagca taatcattca ccacgcaacc agcagcgcgc atcagccaga cacccgcgac
    21601 aaacaccgcc aggatccaga gctgaggaac gcccggtgtc gccacccaca acgcccataa
    21661 tgttggccag agcagcaata acgcgccaat tggcttatcc gtacgcatta agcgatgaaa
    21721 cgccagcagc ttattctgcg tcagactcca ctccatattt ttttcctctt agtacaacgg
    21781 tgacgccggt aaaaacagtt ctgttagcaa cagcggttta ccgcttaatc gcaggcggga
    21841 acgtcgcccc cacagtccgg catcacggcc tatctcgata aaatcccggg ttagtgtcga
    21901 tgatgtgaac agatagcgtc ctaacggcgt tttacccaat ttttgtaacg ctagttccgg
    21961 cccgcttaat gttgatacag gaacgacggt acgaccggca agccacggtt caccatcggc
    22021 acataacaaa atttcacgta accagtaacg agactctttc ggcagtagcg gcagttcttc
    22081 ggggatttca ttctgctcga caaacccctc gcggatcatc gtcacactca ccgtttttcc
    22141 ctgctgttca aaacgttttg tcatggaatc ctccagcaac agccagtcga acagttgcgg
    22201 atccagggca gggatctctt taaaatagcg cagcgcacgc agttgcgtta acgcggggtg
    22261 tgacatgccg aactctccgt tacataacgt aaaggcattg tatcgcagaa aagtgtttac
    22321 gggagatgta aagcaaatta agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa
    22381 gtggtaaaac gctgttgaac cttacgcttt gccgatgccc gtgctggcga gggcaaccat
    22441 aggggcatca ccgcccatga tgctaggaac ggtgatgttt tcctttactt aaataccttt
    22501 attctttata cgagtagctc gccaactcgc agtaaaaatt gggaaaagcg catatttacc
    22561 atgagatttg gagctgaaaa gtgtttcctg agtaagatta ataggcaaca tcttatgtct
    22621 ggatttatag atacacgtgg aattccaatt ggtatctttt gtaggattca acatatcatg
    22681 taagcgctgg gagcttacca ccataacagg cccatcatcg acagaggaga acaggataaa
    22741 tatattatcg tttcgtgtta atttattagt gttaaaggtt cttaactttt cttcagtttc
    22801 tataaatata ggtgaaacat catcactacg aatccactct ttataaagat accaggcatt
    22861 tgctttgatc atatcatccg ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt
    22921 gttattttta tcaaccagcc ctttattcat attaaatctg ttaataaaat ggatcatctg
    22981 ttgggtatcg aaattcacct tactgaatcg atgttgacgc ataaaattat cgaacttgtc
    23041 atcttcccca gaatgtatat aactacgtat aattggatct tttatataag gacaatggct
    23101 aaatgttttc ttcatattct caataaagtt tttattaaca atattgagaa tttttaattg
    23161 catcaccagt tttactgcca gaacggattt caatatcatt gcattatctg gcaatgtatc
    23221 aatcattttt ataatgttat tgatataacc atcgagatct tcattcccaa atatatctga
    23281 ataatcaata gttaattcca attgttctgt aatgcaatct taaaaattcc ctgtcgacat
    23341 tcgcacatga tcaaacataa cagctgtcaa attcgattta ataaatgata tttctttggc
    23401 agacagaaac tctgctctgg tgaagtgcga agcgtaaaga taacaattat cgaagtttga
    23461 atttctaatc tgacattcgc gaaaattact atgaccaagt tgactctgcg agaagtcaac
    23521 tgtatcaaga atattttggc taaaatcaac acattcgcca ctcgttttga gaaaaatcac
    23581 attgtgtaaa cgtcgacatt taaatatgac attatgaact gttgccttag taaaattgca
    23641 attctcaatt tctttacatt cgaaagttac atgttgtaga tttgatgata aaaaagacac
    23701 cttagaaata acgtcacctt taaaatgaca atttgttaaa tcaaaatcag acaggtcgat
    23761 atacccctgc gcatctttat ccatcagaaa attacaagcg cattctattt tttcatttct
    23821 gtagaatttt acacttatag tatcattact aattgagact tcacgtaaca tatttattgc
    23881 atgatttttt tcgttccgtt gttttatatt taaaaatata tcactgatat aaataaagtt
    23941 atctctgttt aaacctgtca ccccattgaa attacgtagt aaatcaagat ctactatatt
    24001 atttccttgc accacatcat gtttcatcaa agagttattg ccgccagaat ttgtgatatg
    24061 acctatcata aaacctctgt aatacagaaa taaaataacc tggaaaagta gcctgagcgt
    24121 ccagacacta ccggcgaaag gaaaagaatc tctgataatg cattgaaata atggacattc
    24181 ttagtgaaga agaagtgaag tactataaaa aatcgctcat atcccaggaa ggaagcataa
    24241 aaaaaggtgc gccaggagac gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta
    24301 ccccttgcct tttacactgc tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc
    24361 agcttcatca agcagtttta acgccttatc aacatcacct ttcgcgacag cgtttttgat
    24421 cgcggtatta aaataacttt ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac
    24481 cggagcaggt gcagccacag ctggtgccgc cgtgttgcct accgtaaccg gagctggagc
    24541 ggaagaacca aatagtggtc ctaccaacac gctggagctg gagttcgttt tgactttcag
    24601 tttcagtaag ccatcgctgg tatgacgagc aaccggatcg ggaatatccg ggatcgagtt
    24661 accgacgcct ttggcatagg ctttagccgg gtcgagcagt tgggtcgtct gctggagatc
    24721 tttttccatg gtaaagacca gaacataaag tttttgctgc cccaacgccg gtgtcaggcg
    24781 cataacgcct tccagccgat ctgcactcat cacgcctagt tcctggtagg tgaaataact
    24841 gctggggaag aaggctgatg gggtcatgtt ctgatcaata atcagcacgt tcggcgcaaa
    24901 aacgctggtt tgtttgttca cttcgctggt cagcgtcagg gtcagttcgc caatgtttgc
    24961 cgggacgctg tacgcagcaa ccggaccact gatgccggga acgctcagtt gttggccgcc
    25021 ggtcgccagt tgggtggtct gggttttaga ttgatcgacc ggtgtccagg tgagttgttg
    25081 cagcgcggca gatggaatgg ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc
    25141 ggcaaggcta attccaggcg cgcttgccag taaccctgct gataaacaga gggcgatgag
    25201 acttttattc attttcattg ttttcacctc aaaatctgga gctcagcggt agccaggcaa
    25261 tagcgcgcta aacccgataa tcagaggggc tttcgcccct ttcagataat gacaacctgt
    25321 ttttatgccg gatgcggcgt aaacgcctta tccggcccag gttttgctat taccaccaga
    25381 tttccatctg ggcaccgaag gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt
    25441 tgaaatcagc aggaacggct ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt
    25501 cgtaacccca tttctcatcc cacttggcgt aggttgcgaa gacacgaata gccgggcgtg
    25561 accagatgct gtcgccagcc tgccattgtt gtgcgagggt aattttgtac tgattgttct
    25621 tgtcgccggt gcgctgggat tcgacgttgt cgtagccgat ttccatcacg gtgctcatga
    25681 ttggcgtcca cttgtacatc gggcgaatac caacggtcca ccacttggtg ccgttgtcgt
    25741 tatcccagtt gatatcctgg tacataccca cgtacatcat gtcccagttg tcgcccatgg
    25801 agatcgcacc gtggtcgagg atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa
    25861 atttttcgtt atcaaacgcg acgccagaac cctgcgacag acctttaccc tgcgaggtca
    25921 tcgagtcagt agcgtactga acaacaaact tgttaaagcc cttcaggaca ctctgagtat
    25981 gttcagcagt gaataaccag ccgtctttcg atgcgccatc aaccagacga tagttatcac
    26041 gcaggttggc acgaccgtag tcgacaccca gttctaatgt gccgcccggg ttgatttcca
    26101 tctgcgctaa acgcacatcg aaaacgtcgt tcgcggtttc gttggtatag tcataaatat
    26161 tgttgctggc aaaagaggaa gaaccaccag cttcagagga gcgggttgct gccagagaga
    26221 gtttaccgaa gccaacatcg atgttttcca gaccggcacc aggaccagaa atatcccagt
    26281 agtagaagtc gatcatatga acgtcatgac gttggtagaa gcgcttacct gcccagatgg
    26341 tggaacctgg cagccattcg atcaggtttt ttccctgcac gtttgcttca cggaaggccg
    26401 gatcggtagc ttcccagtca ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat
    26461 agaagctctt atcgccctct ttccacactt cctgacccaa ttttaattca gcataagttt
    26521 cacattcgtt gccaagacgg tatttacttt gagcaccggt agtctggaaa cactgttgtt
    26581 caccgccgct acctgtccag ccaataccgg aacgtgcata gccgtggaaa tcaacagcca
    26641 ttgcctgagc agacattacg cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag
    26701 taatcatcat tctatctcct gagtcattgc ttttcttttt tcacatcacc tgtgacaggc
    26761 tttgtgtgtt ttgtggggtg cttaaacacc cggctcctta tgcagtcgac gacatgcagt
    26821 gccatcctca cggaacagat ggcaacgctc tggcggcagg ccgatagcga atgtggcacc
    26881 ttcttctacc aacaccacgt cgttctggcg gtacaccagg ttttgacgaa tggaagggat
    26941 ctggatatag atttgggttt cgttgccgag ttgctcgacg acctgaactt caccctcaag
    27001 gatgacgtca gcgatatcac tcggcagtag atgttccggg cgaataccca gcgacatatt
    27061 ggctccaacc tggacatcac ggctttcaac aggcagccag acttgttgac gatttggcat
    27121 cggcagctcc acctgcactt gatcgattgc ggtggcggtc acttttaccg gcaggaagtt
    27181 catctttggc gaaccgataa atccggcgac aaaacggtct gccggatagt ggtacagttc
    27241 cagcggtttc ccaacctgtg ccacgcgacc ggcgtccagc accacgattt tgtcggccag
    27301 cgtcatcgct tcgacctgat cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg
    27361 cagacgggag atttcgatac gcatttgcac acgcagtgca gcatcgaggt tggagagcgg
    27421 ttcatcgagc aaaaatacgc ttggctcggc caccagcgta cggccaatcg ccacgcgctg
    27481 acgctgacca ccggagagcg ctttcggttt gcgatccagc aaatgcgcca gttgcagcac
    27541 ttccgccacc tggttgacgc gttggttaat cacctctttt tttgcgccag ccagtttcag
    27601 gccaaatgac atgttttctg ctactgacag gtggggatag agcgcgtaag actgaaacac
    27661 cataccaacg ccgcgttctg ctggcggagt gtcattcatc cgtttctcac cgatgaacag
    27721 gtcgccgctg gtgatcgtct caagcccggc aatcatgcgc agtaaagtcg atttaccgca
    27781 gccagacggt ccgacaaaca ccacgaattc accttcatgg atatcgagat tgatatcttt
    27841 cgataccacg acctcgcccc aggctttcgt tacattttgc agctgtacgc tcgccatgcc
    27901 cttctccctt tgtaacaacc tgtcatcgac agcaacattc atgatgggct gactatgcgt
    27961 catcaggaga tggcttaaat cctccacccc ctggcttttt tatgggggag gaggcgggag
    28021 gatgagaacg cggcttctgt gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc
    28081 ttgcaaaaat cgtggcgatt ttatgtgcgc atccccacat taccgccaat tctgtaacag
    28141 agatcacaca aagcaacggt ggggcgtagg ggcaaggagg atggaaagag gttgccgtat
    28201 aaagaaacta gagtccgttt aggtgttttc acgagcactt caccaacaag gaccatagat
    28261 tatgaaaata aaaacaggtg cacgcatcct cgcattatcc gcattaacga cgatgatgtt
    28321 ttccgcctcg gctctcgcca aaatcgaaga aggtaaactg gtaatctgga ttaacggcga
    28381 taaaggctat aacggtctcg ctgaagtcgg taagaaattc gagaaagata ccggaattaa
    28441 agtcaccgtt gagcatccgg ataaactgga agagaaattc cctcaggttg cggcaactgg
    28501 cgatggccct gacattatct tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg
    28561 cctgttggct gaaatcaccc cggacaaagc gttccaggac aagctgtatc cgtttacatg
    28621 ggatgccgta cgttacaacg gcaagctgat tgcttaccca atcgctgttg aagcgttatc
    28681 gctgatttat aacaaagacc tgctgccgaa cccgccaaaa acctgggaag agatcccggc
    28741 gctggataaa gaactgaaag cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc
    28801 gtacttcacc tggccgctga ttgctgctga cgggggttat gcgttcaagt atgaaaacgg
    28861 caagtacgac attaaagacg tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt
    28921 cctggttgac ctgattaaaa acaaacacat gaatgcagac accgattact ccatcgcaga
    28981 agctgccttt aataaaggcg aaacagcgat gaccatcaac ggcccgtggg catggtccaa
    29041 catcgacacc agcaaagtga attatggtgt aacggtactg ccgaccttca agggtcaacc
    29101 atctaaaccg ttcgttggcg tgctgagcac cggtattaac gccgccagtc cgaacaaaga
    29161 gctggcgaaa gagttcctcg aaaactatct gctgactgat gaaggtctgg aagcggttaa
    29221 taaagacaaa ccgctgggtg ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga
    29281 tccacgtatt gccgccacca tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc
    29341 gcagatgtcc gctttctggt atgccgtacg tactgcggtg atcaacgccg ccagcggtcg
    29401 tcagactgtc gatgaagccc tgaaagacgc gcagactcgt atcaccaagt aatgctgtga
    29461 aatgccggat gcggcgtgaa cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct
    29521 gataagacgc atcagtgtcg catcaggcag ttgttgtcgg ataaggcgtg aaagccttat
    29581 ccgtcctgga atgaggaaga accccatgga tgtcattaaa aagaaacatt ggtggcaaag
    29641 cgacgcgctg aaatggtcag tgccaggtct gctcggcctg ctggtgggtt accttgttgt
    29701 tttaatgtac gcacaagggg aatacctgtt cgccattacc acgctgatat tgagttcagc
    29761 ggggctgtat attttcgcca atcgtaaagc ctacgcctgg cgctatgttt acccgggaat
    29821 ggctggaatg ggattattcg tcctcttccc tctggtctgc accatcgcca ttgccttcac
    29881 caactacagc agcactaacc agctgacttt tgaacgtgcg caggaagtgt tgttagatcg
    29941 ctcctggcaa gcaggcaaaa cctataactt tggtctttac ccggcgggcg atgagtggca
    30001 actggcgctc agcgacggcg aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg
    30061 cggcgagcaa aaactgcaac tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa
    30121 tctgcgcgtg attacccaga atcgtcaggc gctgagtgac attaccacca ttctgccgga
    30181 tggcaacaaa gtgatgatga gctccctgcg ccagttttct ggcacgcagc cgctctacac
    30241 actcgacggt gacggcacgt tgacgaataa tcagagtggc gtgaaatatc gtccgaataa
    30301 ccaaattggc ttttaccagt ccattaccgc cgacggcaac tggggggatg aaaagctaag
    30361 ccccggttac accgtgacca ccggctggaa aaactttacc cgcgtcttta ccgacgaagg
    30421 cattcagaaa ccgttcctcg ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt
    30481 ctttttaacg gtggcggtcg gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg
    30541 cggcaaagcg gtctatcgcg tcctgctgat tctgccctac gcggtgccat cgttcatttc
    30601 aatcttgatt ttcaaaggat tgtttaacca gagcttcggt gaaatcaaca tgatgttgag
    30661 cgcgctgttt ggcgtgaagc ccgcctggtt cagcgatccg accaccgctc gcacgatgct
    30721 gattatcgtc aatacctggc tgggttatcc gtacatgatg atcctctgca tgggcttgct
    30781 gaaagcgatt ccggacgatt tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca
    30841 gaacttcttt aagattacgc tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc
    30901 cagcttcgcc tttaacttta acaacttcgt gctgattcag ctgttaacca acggcggccc
    30961 ggatcgtctt ggcacgacca cgccagccgg ttataccgac ctgcttgtta actacaccta
    31021 ccgtatcgct tttgaaggcg gcgggggtca ggacttcggt ctggcggcag caattgccac
    31081 gctgatcttc ctgctggtgg gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa
    31141 gtttgattaa gggagataac aaaaatggca atggtccaac cgaaatcgca aaaagcacgt
    31201 ttgtttatta ctcacctgct actgctactt tttatcgcgg cgattatgtt cccgctgctg
    31261 atggtcgtcg ctatctcgct gcgtcaggga aacttcgcga ccggtagcct gatcccggag
    31321 caaatctcct gggatcactg gaaactggcg ttaggtttta gcgttgaaca ggctgatggt
    31381 cgcattacgc caccgccatt cccggtactg ctgtggctgt ggaactcggt aaaggtcgct
    31441 gggatttccg cgattggcat tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt
    31501 atgcgctttc caggcaaagc gacgctgctg aaaggaatgc tgattttcca gatgttcccg
    31561 gcagtacttt cactggtcgc gttgtatgcg ttgtttgatc gtctgggtga gtacattcca
    31621 ttcattggcc tgaatactca cggcggcgta attttcgcgt atctgggtgg gattgcgctg
    31681 catgtctgga ccatcaaagg ctatttcgaa accatcgaca gttcgctgga agaagcggct
    31741 gcgctggatg gtgcgacacc ctggcaggcc ttccgccttg tcctgttgcc gctgtcagta
    31801 ccgattctgg cggtggtatt catcctgtcg tttatcgctg ccattactga agttccggtc
    31861 gcgtcgctgt tactgcgtga cgtaaacagc tacaccctgg ccgtggggat gcagcaatac
    31921 ctcaacccgc aaaactacct gtggggtgac tttgccgccg ctgccgtgat gtctgcatta
    31981 ccgatcacca tcgtcttctt gctggctcaa cgctggctgg tcaacggcct gacggcaggt
    32041 ggtgtgaaag gttaaagatg ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg
    32101 ttatcccaac ttgtgactat tattcggcgc tccacggagc gccttttttc tttcgtctgc
    32161 aatctgaatc gttcgccggt taatattccc atcatagaac ttattatttt tacgttattt
    32221 gttttcccac ttacgataat tctctttcgt gctctgagtc acggcaatag tattgttttc
    32281 atcaattttt gataattatc acaattaaga tcacagaaaa gacattacg
//
LOCUS       DADMGC010000035        32306 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.035, whole genome shotgun sequence.
ACCESSION   DADMGC010000035 DADMGC010000000
VERSION     DADMGC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.035"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            63..695
                     /locus_tag="JNZ73_001581"
     CDS             63..695
                     /locus_tag="JNZ73_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HAY8731904.1"
                     /translation="MKNLPIGGVWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFP
                     EFAHATPRVQLDLHPTGSVNGSNYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAA
                     GRRDGYFSIYRQGGTTTDEGERIDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRP
                     VVLPGIRYAVMCVPTPLTLAVDKFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(783..2081)
                     /locus_tag="JNZ73_001582"
     CDS             complement(783..2081)
                     /locus_tag="JNZ73_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8731905.1"
                     /translation="MSKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            2564..3976
                     /gene="uxaC"
                     /locus_tag="JNZ73_001583"
     CDS             2564..3976
                     /gene="uxaC"
                     /locus_tag="JNZ73_001583"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAY8731906.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHFELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            3991..5478
                     /locus_tag="JNZ73_001584"
     CDS             3991..5478
                     /locus_tag="JNZ73_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAY8731907.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            5561..6112
                     /locus_tag="JNZ73_001585"
     CDS             5561..6112
                     /locus_tag="JNZ73_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HAY8731908.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(6117..7361)
                     /gene="sstT"
                     /locus_tag="JNZ73_001586"
     CDS             complement(6117..7361)
                     /gene="sstT"
                     /locus_tag="JNZ73_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAY8731909.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(7957..8922)
                     /gene="alx"
                     /locus_tag="JNZ73_001587"
     CDS             complement(7957..8922)
                     /gene="alx"
                     /locus_tag="JNZ73_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HAY8731910.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(9193..9393)
                     /locus_tag="JNZ73_001588"
     CDS             complement(9193..9393)
                     /locus_tag="JNZ73_001588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY8731911.1"
                     /translation="MQDLTQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLL
                     TEIRRQTGVIFPADSVKLYLPK"
     gene            complement(9424..10191)
                     /locus_tag="JNZ73_001589"
     CDS             complement(9424..10191)
                     /locus_tag="JNZ73_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005071014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY8731912.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSEC"
     gene            complement(10270..10962)
                     /locus_tag="JNZ73_001590"
     CDS             complement(10270..10962)
                     /locus_tag="JNZ73_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HAY8731913.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(11039..11542)
                     /locus_tag="JNZ73_001591"
     CDS             complement(11039..11542)
                     /locus_tag="JNZ73_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY8731914.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRMQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            11627..12763
                     /gene="rlmG"
                     /locus_tag="JNZ73_001592"
     CDS             11627..12763
                     /gene="rlmG"
                     /locus_tag="JNZ73_001592"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HAY8731915.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            13048..13362
                     /locus_tag="JNZ73_001593"
     CDS             13048..13362
                     /locus_tag="JNZ73_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase HigB"
                     /protein_id="HAY8731916.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            13359..13775
                     /gene="higA"
                     /locus_tag="JNZ73_001594"
     CDS             13359..13775
                     /gene="higA"
                     /locus_tag="JNZ73_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HAY8731917.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(13820..15838)
                     /locus_tag="JNZ73_001595"
     CDS             complement(13820..15838)
                     /locus_tag="JNZ73_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HAY8731918.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLIGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(16058..18409)
                     /gene="ygjK"
                     /locus_tag="JNZ73_001596"
                     /pseudo
     CDS             complement(16058..18409)
                     /gene="ygjK"
                     /locus_tag="JNZ73_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(18426..19497)
                     /gene="ygjJ"
                     /locus_tag="JNZ73_001597"
                     /pseudo
     CDS             complement(18426..19497)
                     /gene="ygjJ"
                     /locus_tag="JNZ73_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(19631..21064)
                     /locus_tag="JNZ73_001598"
     CDS             complement(19631..21064)
                     /locus_tag="JNZ73_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8731919.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGDVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(21127..21576)
                     /locus_tag="JNZ73_001599"
     CDS             complement(21127..21576)
                     /locus_tag="JNZ73_001599"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HAY8731920.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATHALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(21573..24659)
                     /gene="ebgA"
                     /locus_tag="JNZ73_001600"
     CDS             complement(21573..24659)
                     /gene="ebgA"
                     /locus_tag="JNZ73_001600"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HAY8731921.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHDNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(24842..25825)
                     /gene="ebgR"
                     /locus_tag="JNZ73_001601"
     CDS             complement(24842..25825)
                     /gene="ebgR"
                     /locus_tag="JNZ73_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HAY8731922.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            26044..26375
                     /locus_tag="JNZ73_001602"
                     /pseudo
     CDS             26044..26375
                     /locus_tag="JNZ73_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(26417..27907)
                     /gene="ygjG"
                     /locus_tag="JNZ73_001603"
     CDS             complement(26417..27907)
                     /gene="ygjG"
                     /locus_tag="JNZ73_001603"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HAY8731923.1"
                     /translation="MITAFVFIPIFAIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNL
                     PAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRV
                     LVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            28213..29733
                     /locus_tag="JNZ73_001604"
     CDS             28213..29733
                     /locus_tag="JNZ73_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY8731924.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRTRGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(29887..30510)
                     /locus_tag="JNZ73_001605"
     CDS             complement(29887..30510)
                     /locus_tag="JNZ73_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAY8731925.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            30787..31533
                     /gene="nfeF"
                     /locus_tag="JNZ73_001606"
                     /pseudo
     CDS             30787..31533
                     /gene="nfeF"
                     /locus_tag="JNZ73_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(31587..31662)
                     /locus_tag="JNZ73_001607"
     tRNA            complement(31587..31662)
                     /locus_tag="JNZ73_001607"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31627..31629),aa:Ile,seq:cat)
     gene            31787..32293
                     /gene="mug"
                     /locus_tag="JNZ73_001608"
     CDS             31787..32293
                     /gene="mug"
                     /locus_tag="JNZ73_001608"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HAY8731926.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT         7815 a         8428 c         8500 g         7563 t
ORIGIN      
        1 atggtcaaca ttattatata tacgatacaa agtttcttac gctctacctt gagcagacag
       61 agatgaagaa tttgccgatt ggtggcgtct ggaaggggaa agttaaatta cattcgaaca
      121 gcccggccca ggactatttc gcaaatatta ccctgaatac gctcgacccc aaccatattg
      181 acgtgttctt cccggagttc gcccacgcca cgccaagggt gcagttagac ttgcatccaa
      241 caggaagcgt taacggcagc aactacgcgc aagatctgac catgttggac atgtgcctgt
      301 acgatggttt taacggtaat gccatcagtt atgaaatcat gctcaaagat gaagggcgac
      361 cagctgcagg gcgcagagac ggttacttct ctatctatcg tcagggaggg accaccaccg
      421 acgagggaga acgcattgat taccgggtca aaatgtacaa cccggaaacc ggtgggcaaa
      481 ttgatgtgcg caataatgaa aatatggtct ggaacagcat taacctgaaa cgtgtgcgtc
      541 cggtggtact gcccggtatt cgctatgccg tgatgtgtgt gccgacaccc ttgactctgg
      601 cagtcgataa attcagcgtg atggataaac aggccgggta ctacatgggc aaattgtcag
      661 taatctttac gccttccttg ccaaccatca attaaccctg caaaccggac aaacgctttt
      721 ttctatataa acgacgaagc cgcctgataa ggcggcttcg tcgttataac ttacgttaaa
      781 cgttaatgtt gcggtgcggg atcgttatgt gtttcctgtg ccacctcaat cgccggtttg
      841 ttctgcaata cggtccagat aaccagcgca cccagcaggt caaatactgc cagaactgcg
      901 aacagcgggc tgaagccgat ggtgtcagcc agtgcaccga caaccagcgc aaacagcgta
      961 cttgccagcc atgcggacat cccggttaaa ccgttcgccg tcgccacttc gttacgaccg
     1021 aacacgtcag aagagagcgt aatcagcgcg ccagacagtg cctggtgggc aaaaccaccg
     1081 atacacagca gcataattgc gacatacggg ttggtgaaca gaccgatcat acccgggcca
     1141 atcatcagca cagcacccag cgttacgacc atcttacggg aaacgatcag gttcacacca
     1201 aaccaacgct ggaacagcgg cggcaggtaa ccaccgagga tacaaccgag gtcagcaaac
     1261 agcatcggca tccaggcgaa catcgcgatc tctttcaggt taaagccgta aactttaaac
     1321 atgaacagcg ggatccacgc gttaaaagta ccccaggccg gttctgctag gaaacgcggc
     1381 agtgcgatac cccagaactg acggttacgc aggatctgac caacggacat tttcttcgcc
     1441 gtgctcacct ggtgctgggc ttcctgacca ttaataatat agtcgcgttc ttcatcggtc
     1501 agatgcttct ggtcgcgcgg atgtttatag aaaatcagcc atgccatcgc ccagataaag
     1561 ctcaatgcac cggagatgat aaatgccatc tgccagctgt gcatcacgat tgcccatacc
     1621 accagcggcg gcgcaatcat cgcaccaatc gaagaaccta cgttaaagta gcctactgcg
     1681 atggaacgct ctttcgccgg gaaccattcg gagctggctt tcagacccgc cgggatcatc
     1741 gctgcttccg cggcaccgac cgcaccacga gcaacagcca ggccacccca gctacctgcc
     1801 agcgcggttg caccacagaa cacggcccac agcacagcaa acattgcata accgattttc
     1861 gtacccagca catccagtac ataacctgct accggttgca tgaccgtata agcagcagaa
     1921 taggctgcga tgatatagga atactgttgg gtggagatgt ttaactcttc catcagagtt
     1981 ggcgcagctg ccgccacagt gttacgcgtc aggtaaccaa gcacggtgcc gagcgtcacc
     2041 agtgcgatca tataccaacg taacccttta attttactca tgtaaaacct catcgttttg
     2101 ttatatccgc aggaagcgcg gccatcatcc cgccagaggc tgaagatgtt agttacgacc
     2161 gggtaaccga aaaaaaaccg gcacgccccg aacgttgcca tgaaagtttc gtgctaattc
     2221 ggcttccgta ccggttaacc cgattcatct aacaaatgaa tgaatcggca atttgcattc
     2281 cgtcgactta tgatttgcga cggcagaaag ataacttgtc atacaacctt aaaaggtgag
     2341 agccatcaca aatgtgcgaa tatttgtagg gacattacct gacgacagcg aggccagtac
     2401 tggcgcggcc tgcagcgaga tttaccactt tgagagtaat ttttttaact acgtttattg
     2461 atctaactca cgaaaatatc ttcggactct ggaaattggt gtgataactt tgtcagcatc
     2521 gcaccataag caagctagct cactcgttga gaggaagacg aaaatgactc cgtttatgac
     2581 tgaagatttc ctgttagata ccgaatttgc ccgccgtctg tatcacgact acgcaaaaga
     2641 ccagccgatt ttcgattacc attgccattt gccgccgcag cagattgcgg aagactatcg
     2701 ttttaaaaac ctgtatgaca tctggttgaa gggcgatcac tacaaatggc gcgctatgcg
     2761 taccaacggt gtggctgagc gtctgtgtac tggtgatgcg tctgaccgtg aaaaatttga
     2821 cgcctgggcg gcgactgttc cgcacactat cggcaacccg ttataccact ggacgcactt
     2881 cgaactgcgt cgtccgttcg gtatcactgg caaattgctt tctccgtcaa ctgccgatga
     2941 aatctggaac gaatgtaacg aactgctggc gcaggataac ttctccgcgc gcggcatcat
     3001 gcagcagatg aacgtgaaaa tggtcggcac caccgatgac ccgatcgatt ctctggagca
     3061 tcacgcagag atcgccaaag acggctcttt caccatcaaa gtgctgccga gctggcgtcc
     3121 ggacaaagcc ttcaatatcg aacaggcgac ctttaacgac tacatggcga agctgggcga
     3181 agtttccgat accgacattc gccgctttgc tgacctgcaa actgccttga ccaaacgtct
     3241 ggatcacttc gccgctcacg gctgtaaagt gtctgaccat gcgctggacg tggtgatgtt
     3301 tgctgaagcg aacgaagcgg aactcgacag cattctggcg cgccgtctgg ctggcgaaac
     3361 cctgagcgag cacgaagtgg cacagttcaa aactgcggta ctggtgttcc ttggtgccga
     3421 atatgcacgt cgcggctggg tacagcagta ccacattggc gcgctgcgta ataacaacct
     3481 gcgtcagttc aaactgctgg ggccggatgt aggctttgac tccatcaacg accgtccgat
     3541 ggcggaagag ttgtctaagc tgctgagcaa gcagaacgaa gaaaacctgc tgccgaaaac
     3601 cattctctac tgcctgaacc cgcgcgataa cgaagtgctg ggcaccatga tcggtaactt
     3661 ccagggcgaa ggtatgccgg gcaaaatgca gttcggttcc ggctggtggt ttaacgatca
     3721 gaaagacggt atggaacgtc agatgaccca actggcgcag ctcggtctgc tgagccgttt
     3781 tgtcggtatg ctgactgaca gccgtagctt cctgtcatac acccgtcacg aatacttccg
     3841 ccgcattctg tgccagatga tcggtcgctg ggtggaagcg ggcgaagcac cggcggacat
     3901 caacctgctg ggcgagatgg tgaaaaatat ttgctttaac aatgcgcgtg actacttcgc
     3961 cattgaactg aactaaggtc tggggttgat atgcaataca tcaagatcca tgcgctggat
     4021 aacgtcgcgg tcgctttagc agatttggct gaaggcacag aagtcagtgt cgataaccag
     4081 actgttacgc tgcgccagga tgttgctcgt ggacataaat ttgcgttaac ggatatcgca
     4141 aaaggggcca acgtcatcaa atatggcctg ccgattggtt atgcattggc ggatattgcg
     4201 gcgggggaac acgtccacgc ccacaatacg cgcacgaatc tgagcgatct ggatcagtat
     4261 cgctatcaac ctgattttca ggatctgcct gcgcaagcgg cagatcctga agtgcagatc
     4321 tatcgtcgcg ctaacggcga tgtcggggtg cgtaacgagc tgtggatcct gccaaccgtg
     4381 ggctgtgtca acggcatcgc gcggcaaatc cagaaccgtt tcctgaaaga gaccaacaac
     4441 gccgaaggta ccgacggcgt gttcctcttc agccacacct acggctgctc acaactgggc
     4501 gacgatcaca tcaacacccg caccatgctg caaaacatgg tgcgccaccc gaacgcgggc
     4561 gcagtgctgg tgattggtct gggctgtgaa aacaaccagg ttgccgcatt ccgtgaaacg
     4621 ttgggcgata tcgatcctga acgcgttcat ttcatgatct gccaacagca ggatgatgag
     4681 atcgaagccg ggatcgagca tttgcatcag ctgtataacg tgatgcgcaa cgataaacgc
     4741 gagccaggaa aactcagcga actgaagttt ggtctggagt gcggtggttc tgacggtctt
     4801 tctggtatta ctgctaaccc gatgctgggg cgtttctctg actacgtgat tgctaacggc
     4861 ggtactaccg tactgaccga agtgccggag atgtttggcg cagagcagtt gctgatggac
     4921 cattgccgcg acgaagcaac gtttgaaaaa ctggtcacca tggtcaacga cttcaaacag
     4981 tactttattg cccatgatca gccgatctac gaaaacccat caccggggaa caaagcgggc
     5041 ggtatcacca cgctggaaga caaatcactt ggctgtaccc agaaagcggg ttccagtgtc
     5101 gtggttgacg tgctgcgtta cggcgagcgt ctgaaaacgc cagggctgaa cttgttaagt
     5161 gcgccgggta acgatgccgt agcgaccagc gccctggcgg gtgcgggctg ccatatggtg
     5221 ctgttcagta ctggtcgcgg cacgccatac ggtggatttg tgccgacggt gaaaatcgcc
     5281 accaacagtg aactggcggc gaagaaaaaa cactggatcg actttgacgc gggtcagctg
     5341 atccacggta aagcgatgcc gcagttgctg gaagaattta tcgacaccat cgttgagttt
     5401 gccaacggta agcaaacctg caacgagcgt aacgacttcc gcgaactggc gatcttcaaa
     5461 agcggcgtaa cgctataaag tcgaaatgag ttgttaataa aaaacggcgt ttcataatgt
     5521 gaagcgccgt ttgcttttcc atacctgcta aggatttatc atgaccgcgt attggctggc
     5581 ccagggcgtg ggtgtcatcg cctttctgat tggtatcaca acatttttca atcgtgacga
     5641 acgtcgcttc aaaaagcagc tttcggtcta tagcgccgtt attggcgtac atttttttct
     5701 tctgggcacc tatcccgctg gtgccagtgc catccttaat gccattcgta cattgattac
     5761 cttacgcacg cgcagcttat gggtaatggc gatttttatt gtgctgactg gcggaattgg
     5821 cctcgcgaag ttccatcatc ctgtcgaact attgccggtt atcggtacga ttgtcagtac
     5881 ctgggcgctg ttccgctgta aagggctcac catgcgctgc gtgatgtggt tttcaacgtg
     5941 ttgctgggtg attcacaact tctgggcggg gtcgataggc ggcacgatga ttgagggtag
     6001 ttttctgctt atgaatggcc tgaatatcat tcgtttctgg cggatgcaga aaaggggaat
     6061 tgatccgttt aaagtagaga aaacccctcc cgccatagac gaaaggggtt aaacaattaa
     6121 ttacgcaggg cgctatttgc cagacgatcg tcttccgcct ggcaagctgc cgcagtgaac
     6181 agcacgtcag ttgaagagtt cagcgcggtt tcgcaagagt cctgcaatac gccgataata
     6241 aagccgacgg caaccacctg catggcgata tcgttcgaaa taccgaacat attacaggcc
     6301 agtgggatca gcagcaaaga accccccgcc acgccggatg cgccacaggc acacagagaa
     6361 gccactacgc tcaacagcag cgctgtgggc agatcgaccg gaatacccag cgtattaacc
     6421 gcagccagcg tcaacacggt aatggtgatt gctgcgcccg ccatattgat ggtggctccc
     6481 agcggaatag aaactgaata ggtatcgcga tccagattca gcttttcaca cagcgccata
     6541 ttcaccggaa tgttcgctgc agagctgcgg gtgaagaagg catacacacc gctttcgcgc
     6601 aggcacagca gcaccagcgg gaacgggtta cgacgaattt tccaccacac cagcaatggg
     6661 ttaaccacca gcgccaccag taacatacag ccaaccagca cgaccagcag ttgcgcgtag
     6721 ccccacagtg tggagaaacc ggtggttgcc agggtagaag aaaccagccc aaaaataccg
     6781 atcggtgcga agcgaatgac cagtttcacc ataaaggtaa cggcattcga catatcattg
     6841 accaggtttt tggtggtctc gttaccgtga cgcagtgcga agccgaggcc gatcgcccac
     6901 accagaatcc cgatgtagtt acctttcagc agggcgtcga tggggttgga aaccatgctc
     6961 attaccagtc cgcgcatcac ttcgacaatg cctgacggcg gcgaaatatc acccgcgcta
     7021 ctggacaagt gcagggtaga agggaaggca aagctgaaga ctactgcggc cagagctgca
     7081 gagaaggtgc ccagcagata gaggaacaaa ataggacgga tattggtttt ctgcccgtgc
     7141 tggtggttag caatagatgc cattaccagc atcaacacca ggatgggggc aacagctttc
     7201 agtgcgccga cgaacaaagt acctaacaga ccaacagctt ccgccgcggg ttttgagatc
     7261 catgccagaa gaatccccag aacaaggccg accaggattt gttttaccag gctgccatga
     7321 gccagacgcc ggaatagccc cggtgaacgt tgcgtagtca tttttcgatc ctttcattgt
     7381 gttgtcgcac atccctggtg cgttctgtcg acgcatcttt caggatgtaa caaagtgttt
     7441 gcaaggtcga gtataaggaa agttacagct tcgggaagcg aaaaatgctg gattttaaag
     7501 agaggtcatg ttttgcaact taatggttgc atttatttaa cataaacaga gggaaggact
     7561 ggctattaag gctgattaga taacatatgg gcaaaactat ttggaatatg agaacggttg
     7621 cgctccttta acgtgttatg ccatataaag tcactggcgc agggagcgcg cagggggcgg
     7681 ccaatcgccg ccgccccctg cccccccagg ctctggcgaa caaaatcgcc gctgcgcggt
     7741 tccctcagcc tatcccttgc ggctaccggg tcgggcgcaa ggtaacgtcc ctgtaagatg
     7801 cgccctcgcc ccggcctcca gggaacgctt cggcgatttt gacgccacca acacccgtgc
     7861 ggtctttttg taaagagcta taagccgatt acataattaa tagctcacct ttgtctggaa
     7921 tgagttatct ctaggaatgg cttaatatac agcgaatcat tctccccgct gcttatcatg
     7981 ccgataattc acccaggcgt tgataataaa cgtcatcacc agaatgccaa acaccacgcc
     8041 cagcgagacg gcgattggaa tatggtagaa gtcgacaatc agcatcttga taccgataaa
     8101 caccagaatc accgccaggc catatttgag catcgagaaa cgctctgcta cgcccgccag
     8161 caggaaatac atcgcacgca ggccgaggat tgcaaacagg tttgaggtca gcacaatgaa
     8221 cgggtcagtg gtcacggcga agatagccgg aatgctatcc acggcaaaaa tcacgtcgct
     8281 caattccacc agaatcagta ccagcatcag cggtgtggcg tacagcaatc cattcttacg
     8341 cacaaagaaa tgctcgttgt cgatggtgtc ggtcatgcgc aaatgaccgc gtagccagcg
     8401 caccagcggc ttgtcgccaa tgcctgattc atcttcatgg gcgagcgcca tcttcacgcc
     8461 ggtaaacagc aggaaggcac cgaagatata caggatccag tcgaactgcg aaatcagcca
     8521 gctgcctgtg aagatcatga tggtacgcag aacaatcgcc ccgagcacgc catacaccag
     8581 cacgcgacgt tgtaatgccg ccggaacgga gaaatagctg aacaacatca gccagacaaa
     8641 gacgttatcg accgccagcg atttctcaat cagataaccc gtgagaaagg ccagggcctg
     8701 tggatcggca acggcgcgac cttcggtttg caccagatac caccagaaag cggcgttaaa
     8761 cagtaacgac agcgtcaccc agaccagcga ccaggccgca gcctgtttca tggtcatggc
     8821 atgtgcccca cgacgcccct gcaacaacag gtcgatagcc agcataatgg cgacaacaac
     8881 agcaaatccg ccccatagca acggcgtgcc gacagtattc atagaagttc cttacacata
     8941 aaaaaacggc caacgtcgga agacgtcagc cgctgctttt tatgcataga cctcgccttc
     9001 cggcaaggtc tcacttacaa caaaaaagga atacgtctgc gtattccttt cgggttgccc
     9061 ggtgaccgga tgtggttttt cacacatcgt aatgacgatc gaccggcaat gaagttactc
     9121 ccctttgcag gtaacaaagt atgacagacc attcagtcag tcaatggtct gttacatacc
     9181 ttttacactg ttttactttg gcaagtatag ttttacgcta tctgccggaa aaatcacccc
     9241 agtctggcgg cggatctcgg tcagcagttt ggctgtgatg cgactgaccg ccagccctgg
     9301 atgatccacc agatgctcat ccaccagggt agcgaacagc tctgcttcgt agagcatggt
     9361 attaatatgc tgcggctggg tgagatcctg catctggctg ccacgcggca caaagcagac
     9421 tttctagcat tcagacagtt tttcaatcac cagcgatccc gcttcgccct gaatctcgct
     9481 tcccaggaca gaatcactga cctttgagtg ctgcaaggtg acgctgaaat caccgtaatc
     9541 catcaccacc acgccgtggg cgtcaacgcc gcttgccagc aaactcgccg ttgcctgcac
     9601 gcttttcggc tcaccaaata acgccaccgc cgacgccagg cagtaaaagc cgatatccat
     9661 aattgaaccg ttagagaatg ccggattaaa ggtgttggga ttctcaccgt ccaggtaacg
     9721 ttgataccgc gaggaatact ggcaatagtt gaaaaagact ttacgcagtt tgcctgcttt
     9781 cggcagcgcc tggcgcaaca aatgaaagtt cggcaggcag gcggttttaa atgcctcaaa
     9841 cagcaccacc tgattttcgc gcgcacaggc aatggcggca tccacttccg ccagattcga
     9901 cgccagcggt ttttcgcaaa tcacatgaat tttatggcta aggaaaagtt gtgtctggga
     9961 aaagtgcagg gaattcgggc tggcaatata caccgcgtca atggcatcgc tttccgccat
    10021 cgcttccagc gacgtaaaca gatgctcgac agaaaaatca ttggcgaagt gctgagcctg
    10081 ctcaaggctg cgggaatata cggcggttaa cttgtattta ccgctctcat gggcggcctc
    10141 gacgaactgg cgagtgatcc agttcgtacc aatcacagcg aaacgtatca taacgcgtgg
    10201 atactccata aagggattct gtcgtcaatg tagcacgcct ttttcatctt cgacgtgcgc
    10261 agatacgcat tattctttcg ccggacagcc atgctcacta aacggcccaa tcatgaccga
    10321 tggcccgaga tatttcggct ggcgatggag gatttttgcg tcaatcaccg cgctgacacg
    10381 agcgagtttc tcccgcagtt gagtataaaa accatgcttc atattgaggt ctggcgcgtt
    10441 aaacccgata gcgtcgatgc cgtactgttt tgccagccag atggcgcgtt ggttatgaaa
    10501 ttcttgcgag ataatggtga tatggttttc cccaaacacc tttttggcac gcactaccga
    10561 atccagcgtt gagaatccgg catagtcgca aaagatcact ttcgccggta cgcctttagc
    10621 gatcaaagcc tgctgcatac ctgatgcttc atcgtaattt ttacgtccgt tatcgccact
    10681 caccagtagc catttcactt ttccggcgtg gtacaacgct gctgcggtat caatacgtcg
    10741 ggtaaagtag cgattacccg gcctcgcccc cagcaataaa cccacgttac gcgccgggac
    10801 ggcgttgaca tcgccccagg tcagctgctt gctggcgttg accataacgc gatcggcgat
    10861 aaaaatggtc gctcctgcca caagtaacag taagcaggca atctttagag tccggcgaga
    10921 aaaacaaata cggagaagaa ggcgggcaaa tgcgcgcagc atggtcgtta tccttaacga
    10981 aaggggggag gtcgatttta ggcgctaaag aaagaaaaga taacgcctga catcattttc
    11041 agattttgtc ctgcccaagc gacaactgcg ttagccacag ccgggtgtcg aactcaagct
    11101 ggtggtactg cggctccata tggcagcaca attgatagaa cgctttgtta tgctctttct
    11161 ccttcaggtg cgccagctcg tgcaccacga tcatgcgcaa aaacggttcc ggcgcgttgc
    11221 gaaacacggt agcgacgcgg atctctgctt ttgctttcag cttgccgccc tgcatacgag
    11281 aaacagcggt atgtaacccg agcgcgtttt ttagcacatg gatcttattg tcatacatca
    11341 ctttattgat cggcggggca ttacgcagaa actgattttt cagatcctga gtatattgcc
    11401 agagagcttt atcggtggcg tagtcgtgtg ttcccggata gcgttttgcc agcacatcgc
    11461 ccagacgctg ttcgttaatc agtgttcgca cctgcgaaag cagctgctcg ggataaccct
    11521 gaagataagt aagattgctc ataaccgccc acgaaaaaga gaaaaagggt atactcacgc
    11581 acccttttca ggggcaacgt cgaaatttta tcattcagga gggccgatga gccacttaga
    11641 caacggtttc cgttcactga cactacaacg ttttccggcg acggatgacg ttaacccgct
    11701 acaggcgtgg gaagcggcag atgaatattt gctgcaacag ttggacgaca cagaaatccc
    11761 cggcccggtg ttgatcctga atgatgcctt tggtgcgtta agctgcgcgc tggcggaaca
    11821 taagccgtac agcattggcg actcatacat cagtgaactg gcgacgcgcg agaatttacg
    11881 cctaaacggg attgatgagt cgagcgtgaa gtttctcgac agcaccgccg actacccgca
    11941 acagccgggt gtggtgctga tcaaagtgcc gaaaacactg gcattgctgg aacagcaact
    12001 gcgtgcgctg cgcaaagtgg tcacgtcgga tacacgtatt attgccggtg ccaaagcccg
    12061 tgacattcac acttccacgc tggaactgtt cgaaaaagtg ctcggcccga ccaccaccac
    12121 gctggcatgg aaaaaagcgc gcctgattaa ttgcacgttc aatgaaccgc cgctggccga
    12181 tgcgccgcag accgttagct ggaagctgga aggtactgac tggactatcc ataaccatgc
    12241 gaatgtcttc tcccgcaccg ggcttgatat cggcgcgcgc ttctttatgc aacatctgcc
    12301 agagaatctc gaaggggaga ttgtcgatct cggttgcggt aatggcgtta ttggtctgac
    12361 gctgcttgat aaaaacccgc aggcgaaagt ggtgtttgtc gatgaatcgc cgatggcggt
    12421 tgcttccagc cgtttgaacg ttgaaaccaa catgccagag gcgttggatc gcagcgagtt
    12481 tatgatcaac aacgcgctct ccggcgtgga gcctttccgc tttaatgctg tgctctgcaa
    12541 ccctccgttt caccaacagc atgcgctgac cgataacgtc gcctgggaga tgttccacca
    12601 cgcccgccgc tgcctgaaaa tcaacggcga gctgtatatc gttgccaacc gccatctgga
    12661 ttacttccat aaactgaaga aaattttcgg caactgcacc actattgcga cgaataataa
    12721 atttgtggtg ctgaaagcgg tgaagctggg acgtcgtcgg taagattggg agttacctgc
    12781 cggatgcggc gtaaacgcct tatccggcct acgttcagca cgttaccccc aggccggata
    12841 agacgcggca agcgtcgcat ccggcaatgt ctgctcagtt ctggaatcgc gcttccataa
    12901 aacaaaaaat ccctgtcacc atcctctaca ttctcttgtt tagcgttttt ctacgtttat
    12961 tcttccgtca cacagataaa ttcatccgtt gccaatctgt caacggatgt tatcatattg
    13021 ccaatttgtc aacggacgtg atgaagaatg cacctgataa ctcaaaaagc attgaaagat
    13081 gctgcggaaa aatacccgca acataaaacg gagttggtgg ctctggggaa cacgattgct
    13141 aagggatatt tcaaaaaacc tgagtcatta aaagcagtat tcccatctct ggataacttc
    13201 aaatatctgg ataagcatta tgttttcaat gttgggggca atgaattacg tgttgtagca
    13261 atggtctttt ttgaatcgca aaagtgctac atacgtgaag ttatgacgca taaagaatac
    13321 gatttcttta ccgctgttca tcgtactaag gggaaaaaat gattgcgatt gccgacatct
    13381 tgcaagcagg agaaaagcta actgctgtgg caccttttct ggcgggtatt cagaacgagg
    13441 aacaatacac ccaggcgctg gaactggtag atcatctgct gctcaacgat cctgaaaacc
    13501 ccttgctgga tctggtgtgt gccaaaataa ccgcgtggga agaatcagcg cccgaatttg
    13561 cggaatttaa tgccatggct caagccatgc ctggcggtat cgccgtgatt cgtaccctta
    13621 tggatcaata tggtttaacc ctttccgatc tgccggaaat tggcagtaaa tctatggtgt
    13681 cacgcgtttt gagcgggaag aggaaattaa cgctggaaca cgctaaaaaa ttggcaacgc
    13741 gattcggcat ttctcccgcc ttgtttattg attaacgcga cgtgccggat gcgttgcatc
    13801 cggcgcttca aataacgatt taaatctcca gcgccagccg tgttccctgg gcaattgccc
    13861 gtcgtgcgtc cagctccata gccacatcgc agccgccaat taaatgcaca gttttcccgc
    13921 tatcaatcag cggttgcgcc agcgcgcggt ttggctcttg ccctgcgcag atcaccacat
    13981 tgtccactgc taatacctgc gtttcgccgt tgatcaccac atgcagcccg tcatcgtcaa
    14041 tcttctgata acttacgcct gggatcattt tcacaccccg cgagagcagg gtggtgcgat
    14101 ggatccagcc ggtggttttg cctaacccct gtcccggttt gctggctttg cgctggagca
    14161 tcacaatttg ccgtgggcta cgcgggatct gcattccctg cgggcttaag ccaccagcct
    14221 gttgtaggct gctgtcgatc ccccactcat tacagaacct ggcgatattc tggctggtgg
    14281 attcgcccgg ctgacttaaa tacatcgccg tatcaaaacc aatcccgcca caaccgatga
    14341 tggcaacctt gttgcctacc ggcgctttgt cgcgcagtac atcgagataa ctcaatacct
    14401 tcggatgatc gatcccgtcg ataggcggaa cacgcggcac gatcccactg gcgaggatcg
    14461 tttcatcgaa cgcctgtaac tgatccgccg tcacggtgtg attgagtttc agcgtcacgc
    14521 ccgtcacttc gatcatccgg cggtagtagc gcagcgtttc gtaaaactcc tctttgccgg
    14581 ggatctgttt ggcgatatta aactgcccgc cgatctcgct gtgcgcatca aacaatgtta
    14641 cctgatgccc acgcgccgcc gcgttaatgg caaacgccag cccagcaggt ccagcgccga
    14701 ccaccgccag attttttttc tgcacggcgg gaaggattgg cattttggtt tcgtggcagg
    14761 cgcgaggatt caccaggcac gaggtgactt tgccaacgaa gatctgatcg aggcaggcct
    14821 ggttgcagcc aatacaagtg ttgatctcat cggctcgccc tgattgcgct tttgacagca
    14881 gttccgcatc cgcgagaaac ggtcgcgcca tcgatactat atcggcatcg ccgcgcgaga
    14941 gaatatcgtc ggcaacctgc ggatcgttaa tccggttggt ggttaccagc ggcagcgaga
    15001 cgtggccttt cagtttgcgc gtgacccagc taaatgcgcc gcgcggcacg ggcgtggcga
    15061 tggtcggaat ccgcgcttcg tgccagccta ttccggtgtt gataatggtc gcgcccgccg
    15121 cttcaatggc ctgcgccagt tctaccgttt cggcaaaagt gccgccgcct tcgaccaggt
    15181 cgagcatcga caaccggtaa ataataatga agtcgttgcc gacgcgttcg cgcaccgtac
    15241 gcactacttc tacggcaaat cgcatccggt tgcggtaatc gccgccccac tggtcactac
    15301 gctgattggt gcgcagcgtc agaaattcgt tgatcaaata cccttcagag cccatcactt
    15361 cgacgccatc gtatccggcc tcccgcgcca gttgcgcgca gcgggcgaaa tcgtcgatca
    15421 gttgcaggat ctcttcatgg gtgagttcgt gaggaacgaa acggttgatg ggggcctgca
    15481 atgcggacgg tgcgaccaga tgcggttggt agctgtagcg cccggtatgc aaaatttgca
    15541 gggcgatttt gccgccttcc tgatgtacgg cttcggtaat ggtgcgatgg tgtgggatct
    15601 ggctggcgtc gttgagcatt gcgccacctt ccatgccaac gcctattaaa tctggtgcaa
    15661 tgccgccgct gacaatcagc gccacgccgt gacgggcgcg ttcggcataa aacgcagcca
    15721 gccgttcagc accgtccggg tattcctcca gcccggtgtg cattgaaccc atcaacacgc
    15781 ggttttttaa cgtggtaaaa cctaaatcca gcggggcgaa cagcgacggg tagctcataa
    15841 aattgtccag tatgtaaaat aattgttacg gatgagttct aatttagccg tcgatggaga
    15901 aaagggaaaa ggggagtgcg gggtttgtga tgggattcaa aaagaatacg cccggtggta
    15961 acgccggatg cgacgcttgc cgtgttttat ccggcttggg ggattgcaca tgtagggcgg
    16021 ataaggcgtt cacgccgcat ccgccagccg ttgcaaatta ctgcttacgg aaaaaatcgt
    16081 tatacaacat atacaaatga gcggcactcc aggagaaatt tggtgcgcct tgctgcgcgc
    16141 ctgtcagcgg gttgtaattc tcctgaattg ggccatcggc ggttaatcct ttggcgtgcc
    16201 tgaagaacgt atccgccagc ttcagggcat catcacgata accgtaacgc tccatccctt
    16261 tcagaccaaa ccagaactga tccacccata cgcgcccgcg ccagtagata tcagcgccaa
    16321 aagccggatt ggttaacgcc gccgttccca gcggaacaaa ggtgttgaac tctttaggat
    16381 cgagcatcac cttcaccacc gcgtcggcat tggcctgcgt tgccgcaccg ttaaacagcg
    16441 gcgaccagcc ttccggccct ttaccgcgct caacaatcgg tttgcccgcg cagccgttcg
    16501 ccagtggttt atcttcaata cgtacgtcat agtagaactg ggtagtcggg tcgaacatac
    16561 aggtgttgat gtagtccgcg agctgctgtg ccaactggcg atagcgtttg gcctcttctg
    16621 gcttaccgag gatggttgcc atctccgcca gataatggtt atcgctgtac atatagctgg
    16681 cctgatccac cgactcctgc aatagcgagt agcccagcaa cgttccgtcc tgactgcggt
    16741 tttcggcgaa tttcaccgtc tagtcgctac gtttgccgcc attagcgaca tatttatcca
    16801 actgttcttt gtcgataaaa ccaaagacgg cggcatcatc acgccccgat tcccacgacg
    16861 cagcgacctg tgccggaatt tccagactgt cgtactggcc tttctccacc acgcgggcgt
    16921 agttgttcag cccagactgc gtctcttctt tatcgccttt tttcaccgta aacagcatct
    16981 cgccgctctc ggtgttgtgg gctttgtcgc gggtcgcgcc atattccggc accccgttgc
    17041 cgttatgatc gcggttacgt aaccaccagt cgtgataggc caccagtttc gggtacatct
    17101 ctgccaacca ggctttatcc tgggtgacgt tgtatacttc catcaccgac caggcggcaa
    17161 ggctgggttt ggtattacgt tcgttccagt tgccgccatc gccaccatgc tcggggctaa
    17221 ggttccacgc tatcaggtcg gggacaaagc ccacatcctg cggacgcacg ctgtcgccag
    17281 gctggatctg ccaggagaag accgcgcgga tattctcttt ggcgatatcc ggattgaaat
    17341 gcgccatcgc aaacgcctgc ttccaggtat cccacggcca ggtctgattg ccggagaacc
    17401 agcgtccggt caccgacggc gtgacggtgt tataattcac cgcaccgccc ggtgagcgcc
    17461 agttaccgtt gagcgtttcg atcgctttca ccgcgacgcg cgtctgttcc ggcgtcgcat
    17521 ccggattggt taaccctttc ttcaggtatt cttcccagcg ttgctgcgag gcggtgagat
    17581 aaaacgccgg acgcgccaga atatcgcgga tctgcatttg ctctttgcta acttcctgag
    17641 cggtcagcag atgggaatag gtggtgtaga gcgtggtcga accgttgata tgcgccttac
    17701 tggtaaagcg attaccgttg atttcagtct gcaccggcag ggatttatgc acctgatatt
    17761 ccgattcgcc ggaggtcagc agatcccagg tggcgcgcac tttgccaaag gtgactttca
    17821 ggccatcacg ggtggcgctg attttgcgct gatagtcagg gtattcgcca gcaatggttt
    17881 tatcggaaag cggtttccct tctttcgctt ccagtttttc cagcagttcg ccatcccaca
    17941 ccagatccag cggtttattg ctgatgattt tagtttccag tagtgacgtg cgcggcgtgg
    18001 cgaagcgcag agtcatttcg acctgcacat cttttgctgt cagtttttgc accagcgcgc
    18061 cgggaatact gtatgcctcc agcgtgaagt tgactttctt gccgtcttgc cagacagtca
    18121 ggcggtcgaa attgctggcc ataaagttga tgtattcttc cgtcagcagc gcaacgcccg
    18181 gaaagccgcc catggtgttc gggccgtctg gcaacagatg accatgccag gctccgagat
    18241 cgaaaaacgg attaaaacgc tggtgatcgt cgtaatcgta atccttcatg tactgcggcg
    18301 caccagtacg gttaatcacg tttttataat tgtcggcgtt agcggcatga gcggaaaaac
    18361 ttatcagcag agcgcaggtt actggcgtta aaatagtttt gattttcatg aaattacagt
    18421 ccttattacc agtagaaata ttgcatcagg aagatttggt cgctgggatc gttagaggca
    18481 tttttcatgt agaagcggct atcaaaagcc gtggcgaaac gtccgccgcc atattcgtac
    18541 cagataccaa actgcatgaa agaggtggtt tcatcgtcag cgtggtcagg gcgatgtttg
    18601 gcgtaggtgt aagcggtgga gagataaacg ccctttaacg cttcatacat ggcgctcacc
    18661 atcatcccgc tttcacttcc cggtacgtcc agcccgtcgg cacgtccggt atggtgccag
    18721 gcgcgagcgg caaagttagg ggtaaacagg cccgtgagat agagttgtcc tgtgccgtcg
    18781 gtaatttcca gaccattcat ccaggtgatg ccttcggcaa ggtcatactg gatgtaaccg
    18841 ttcatccatt gccggatagg tgtatttgtc gtcgtagcga tcgtacttgc caaaatgtag
    18901 ccaggctttg ctttcgtcat gatgtccggc aggtgtggcg gtgacgctat aacgaaagtt
    18961 gccgcttaag ttctgtacct tcagcgcggt cataatgtcg cgggtattgg gaatgacgta
    19021 gcccaaatcg ggtgtgaagt cgccccacca ttgcaggtca tccagcgagg aatcctgacg
    19081 cagactgagc atgatttcgg tgccatcatt ggtacgataa ccgccataga aacggttaat
    19141 cccaccttcg aaaccacccc agctatggtc aggcacctag gcattaccgt cgtggtcggc
    19201 ctgcacggtc cagccttcgc cgtaaatcag gccgaaccag gagccgtact tcatctccag
    19261 gccaccttca atataggtgc cgtcgctgta tttatgttta ttctcaccct ctaaatccat
    19321 atatccacca agccctaatt cgccgtaaaa acgaaatgaa ggagaagatg tcgaggtaat
    19381 tgcgggtgtt tttgtgtcgt catgttctgc cggacgtgct tcttctcctg ccgcagaaaa
    19441 ggcgtagcaa aacggcagag caagtaatgc tctgcatggt gcagtgatta atttcattgt
    19501 gttaatccat gagctaaaga aaaagtacag ccttttatcg ttgtaattat aaaaatataa
    19561 aggcagaaac tattttccct ctcctcatcc aggaagaggg aaaatgcctg ctcatttttt
    19621 attagtctgc ttaatcaaca ttgctggctg gcgtcgcttc aatatggcgc tcttcagccg
    19681 ttaatccctt tatatattta ctgtatttcc accacgcaaa gccgaggaag ataacaatac
    19741 cgccgacgtt ataaaaaatg atggtcagaa tattcgcgcc agtcgggaat gtcgaagcga
    19801 caaaccctac ggcaaatatc gcaatcagca ttgaaacaac gataataccg gtgcggcggg
    19861 agcccatacg gaaatcgcgt ggcaggtgat ctaatttggc gcgtaaattc aggtaagcca
    19921 gcatgatgaa taacggcgga agcatggacg ctgcggcggt catattaata atggtattca
    19981 tcagatcctg cacggtattg gaaccgagca tcgggataat catcagcggg atgacgatca
    20041 gaaactggat ccatgctgcg cgcgccggaa cgccgttttc gttaagttcg acggttttct
    20101 taccaaagat gccttccggg atttcggaga agaaaatttt caccggcgtt gcggtccaca
    20161 tcagcaggga accgaacatt gcggtaaagg acaccagacc gacaaagcga ttcatcaacg
    20221 cttccggcag accaaaatac gccgccatgc cgtggaatac ctgcaccgat ccgccggtaa
    20281 atttcaactc tttgctgctg acgaagacgt taatcagcac cgaggagacg gaatacagta
    20341 cgccgataaa aatcccggcg aggatgatca ctttaacgaa cgatttcgaa ccgcctttga
    20401 cgtcgttaac gtacaccgcg acggactccg cgccacctgc ggccataaag atccaggtgg
    20461 taacgccgag gaacgcccag ttgaagttcg ggatcatcgc gtcaacggtg atcggatcgg
    20521 caggctgtac gtcgccaacc agtgccgtac ctgccagtaa aatgtaggag agcgttaaca
    20581 gcagcatcag cgttgaagtg acggaggtaa ttggccccag cattttcgcc ccgttggtgg
    20641 aaacccaggt ggagaaggcg aacagcacca tactgataac ggtggtggca accggcgtca
    20701 taatatattc gtagccgagg aaggcatacg aagcataagc aataacgcgc ggcaacagcg
    20761 aggtgaaaaa gaacagattt acgaaccagt aagtgtaggc agtaataaat gcccaacgtc
    20821 cgcccagcga actttttacc cacgcgtaga caccggcttc tgagttttta tttaacgaaa
    20881 caaattctgc gatgatcaga cagaagggaa taaaataaaa aatcgtcgcg aggaaaaaca
    20941 tcggtgccga ggccagtcca agctcaatat tattattgat aacgttgtta aagctgtaaa
    21001 cggcggcaaa agtcagcgag agcaagccga atttgccgat tgtattacgt ttggtatcag
    21061 acataacaca tctccgaaaa tgtgtagggt agcggtggcg gcaaactcct tttgccgccg
    21121 tagttgttat ttgttatgga aataaccatc ttcgatggtg actttgagaa ccactttttt
    21181 gaccgcgtta ttgcagataa accgatacgc ttcatggata tcgcaaataa cgatttgccc
    21241 ttcgtggacc tctacggttt ctccgcagcc ttttaaatat tcacggtcag tttcatcacg
    21301 ataatattcc actacctgta atgtctcttt cggcgcatat tcaatttttt gctgcccttg
    21361 caggtagtaa tgcacttcaa aatagcgtcg atgcccggta aatagcgcat gggtcgcgga
    21421 gtcattttcc acacggtagg tcaatgaatc accgatggag tgggcgacgc caggctgaat
    21481 gttgtcgata ttttcaatcg cttcaacgca gcgttgccac ttcttgccag aggcgtaaat
    21541 ctggcggaac tgttctaagt tatcgatgat cctcattgct tattctcgct gtgcaaattc
    21601 gtggaaaaga accctgcgcc gaactcatac gacgccaggc tttgcgcggt agcttctccg
    21661 ccagaaaccg gcagcaacgt aaagccgtag ctgaagtcac ggaaccagac gcgccaggag
    21721 tccagcacct cgctgcccca ggagttggag ccgaggccaa gtagctggtg gtcgaggttc
    21781 agggtgatgt catcactgcg ctgcagctcg ttacagtgct gggcagcgtg gatgttttcc
    21841 tgggtatagt gccaggcgct gaagttaatt gggcgctgcg gaaccaccag cagaccgttg
    21901 tcgtggcggt tagtcagtgc cgtccagcgg acatgctgac ggttgccgtt gttctgcggg
    21961 aagggatagt tctcgaacat ggcatcgacg gtgctgcgcc agatatcgat gatgttagcc
    22021 tgctggctgt cggcgtagtt ttcgcccggt ccacgaccgt aatacgccac ctgatcgtat
    22081 tcgccgttaa tccccatggt gaaaccgatg cacggaatga tgtgcggata gtcgccgtaa
    22141 cgctcgccgg aaagcgccac gttaacctgg ccatcggcag cgatgcgcca gatgtaggta
    22201 cagcgcatcc cgaagtcaaa caccggcggt gcaataactg tgcggctgat gatcaacact
    22261 tcaccatcgc tctgttctac ggcaaagtcg cgcagatgtt cctgcatgat ctgcaaatga
    22321 ttcggctgcc acaacccttc gtactcctgc ttgtggttgt cgatcatcgg cttgaagaag
    22381 ttgatctttg gctcgcgggt cagcagcgac tcgccattta cctgccagga tgtcggtttg
    22441 ccactcattt ttgagaaggt gatcgcgaag ttgtagccgc gaacggtgca gctcaaacga
    22501 tcgtcttcca gcgtcagcgg acgcgcatta tttggtgcga aaggcactgg ctgcgcggtg
    22561 ttttccttca gcgggaactg ataagtggcg atagaatgcc cggcttcgct gtagcgggtg
    22621 cgggaatctt tggtcaccgt aatgttgagg aacgcttcgc gggcgtccag ctgcggcagc
    22681 gtgatctgca agggggcttc gctgttcggc gcaacgtcgc gcagtttaat ctgctgcgtc
    22741 gcgagcgttt caccttcggc gcgcacctct gcgtgcaggg tgtagtcatc aagcgtggta
    22801 aaccacagtt tattttcgac tttcaactcg ccgcgagtca gcgcgtggat ttttaccggc
    22861 gcgataacct gtttgtactc tttcaggccc gatcccggcg tctgatcgga atagatcaaa
    22921 ccatcaagac agaagttata gttgttggga tagtcgccgt agtcgccgcc gaatttatac
    22981 cagacattgc cgtggtcgtc ctgtgcctgg atcccgtggt cgcaccactc ccagacataa
    23041 tgaccctgaa tgcaatcgtg cttatagaag acgttctggt actccgtcag cccgcccggt
    23101 ccgttcccca tcgcatgagc atattcacag atgatgcgcg gcttcggatg cgggtattca
    23161 ccaaactcat tcatcagcgg cacgcgggtg tacatggtgg aaataatatc gaccacttca
    23221 gcatcgcgat cttcttcgta atgcaccagt cgcgtgtcat ccagcgcttt cgccgcatgg
    23281 tacatcgcgc ggatgttaca gccatagccg gattcattgc ccagcgacca gatgatgatc
    23341 gacggatggt ttttctgtgc gtggatatgg cgaacaatgc gctcgacgta gactttttcc
    23401 cactgcggat cgtcggtaat acggctgata tcgccgacat tagcaaagcc gtgcgattcg
    23461 acgtcggttt ccgccatcac aaacaggccg tagatatcac acaattcgta aaaacgcgga
    23521 tcgttcgggt agtgagcggt acgcacggag ttgatattgt gctgcttcat caactggaga
    23581 tctttctcga cgcgatccat tccaacggcg cggcctttgc gatgatcgtt gtcgtgacgg
    23641 ttgacgccgt gcagcatcac ataacggtta ttgatccaga acagaccgtc gcgcactttg
    23701 atatcacgga agccaacgcg ttgtggcacc acttccagaa cgttgccgtt ggcgtctttc
    23761 agcgtcatga ccagatggta aagataaggg gattctgctg accattgctg cggctgttcg
    23821 acagtaaaag caaagctggc gctggtcagt ttttcaattg ccaaatgatc aatggcgctg
    23881 ctgtgcacca cgcgttcgcc atcaaacagg gtatattcca gcgtcgtgac gacaggggag
    23941 gcggcgagat tttccagcac cacttcgcag gaaagcgtgg catcgcaata ggcttcgtca
    24001 aagtcggtac gcacagtgaa atcgttaata tgcgttaggt gttttccgac cagataaaca
    24061 tcgcggaaga tccccgccga ccaccacata tcctggtctt ccacgtaggt agagtccgcc
    24121 cactgcatca cgcgcacaca caacaggttg tcgccggttt taaccatcgc gctgatgtca
    24181 aactctgcgg tcaggcgact gcccttgctg aaacccacat actgaccgtt aacgtagact
    24241 tcaaaatagg tttcgacgcc gtcaaattta atcagcgtct gtttaccctg ccagccgtcg
    24301 ctgagggtga aaatacgttg ataggcaccg gttgggttat cgctggggac aaacggcaca
    24361 tcgatgggga acggaaaacc ttcgtcggta tattgcagtt tgccgtgacc ttccatttgc
    24421 cacatggcgg ggacggtaat atgcccccag tcagccatta actcagaggt gaaggcttct
    24481 ggtacttgca gcggatggtc aaaaaagtgg aaattccact gaccgcttaa gggcagaaac
    24541 aggctgctgg tttcgcgggc aaaggtacgc gcttgcgcaa cagaatcata tgaaaaaaag
    24601 tacgcacgcg gcgcaagtcg gttttcgtgg gtgagctgaa tgttttccca gcgattcata
    24661 ggacctcccg gtagagagaa atcggaacca ttcatggtga tggtagtttg tgtaattgct
    24721 gggggggtta gtaaaatttt tactaaaagt agcaggtaaa tgcatttact ttaactggat
    24781 cacgaaaata aacggcagca ctgtccggca aaaatgattg ccggacagtg tgtaagggga
    24841 tttaacgggt cgtgccgcgc agttttaatt tgctgggaac gaagactagc agcggcagcg
    24901 cgcgaccatc gcgggctttt tcatacacca ggttaacgcc ctgacttccc atcatctcgg
    24961 aatggatgcg cacggtggag agcggcggaa aggtaaatcg cgcggtgggg atatcgttaa
    25021 cgctgataag cgaaatatct tgtgggatgt tcaggccacg ttcatgaatt gcccgcagta
    25081 cgccgatagc aatggaatcg gaagcaacaa acagtgcctt cggatagtct tcccgcgcca
    25141 gcatttgttt tgccagttca taacccgacg aactggaaaa accgccgcgc cagatatcct
    25201 cttcgcgtac cacttgtttc agtcggccat attccgcaaa ggcgacctca cgaatatccg
    25261 ccttgccagg ctcatcttca ccgccaataa aaccaatacg attaacgccc tggttgatat
    25321 agaagtcgat gatttcttta ctgatgcgtg ccagatcgat atccaccgca tcgtaaccgc
    25381 tgccgggttc gtgaaagtcg ataaaacaga tattgtcggt caacgcgctg gcagcggcgc
    25441 gcagggcggg cgtgggtttg ccgacaatta aaataccggt gacgttttta atgtctggta
    25501 atccgttgtg ttcataacag ttggtcagtt cgatcgccag cttttcgcac tgagtttcaa
    25561 tgccgtggcg gatcgccaga tagtaaggat cgttgatctc cagctcctgc tggtagctgt
    25621 agatagccag aatatggtgt tggttgactg cgcctgtctg tagtttacgg gcgctactgg
    25681 tcttgtactc cagcttttcg gcgatctcga gaatgcgatg tttcgtctct tctttcacat
    25741 tcaatgtcgg atcgtcattt aagaccctgg atactgtcgc cagggatacg ccagcttcga
    25801 ttgcgatgtc ttttaatgtt gccatgttta ccttttttac tttccctgat gtgcgccccc
    25861 attgtagagg aagtgcttat cgggtcacct gttttcagta aataaagcgc gtttctttga
    25921 cgctgtaacg gtaacacagc ggcttaggaa tgtgatcacg cctgcattct ccatttttag
    25981 cctacaggat tgagaaaaac ttttgctata gtttagtaaa attttacccg aagggaggaa
    26041 gcaatggaaa ccgtggctta cgctgatttt gcacgtctgg aaatgcgcgt cggaaagatt
    26101 gtggaagtga aacgccatga aaacgccgac aagctgtaca tcgtacaggt tgatgtgggg
    26161 aaaaaacact gcaaaccgtg accagcttgg tgccgtacta cagcgaagag gagctgatgg
    26221 ggaaaacggt ggtggtctta tgcaatctgc aaaaagcgaa aatgcgtggt gaaacgtcgg
    26281 aatgcatgct gttgtgtgcg gaaaccgatg acggcagtga aagcgtgttg ttaacgccgg
    26341 agcggatgat gcctgcgggc gtacgcgtcg tataaaaaga tcggatggcg acgtcgtatc
    26401 gccatccgat ttgatattac gcttcttcga cacttactcg catggccgcc agcgccttac
    26461 gcgccgcttt gatcaccagt tcacactgtt caatggtcag tgtcagtggc ggttcaatgc
    26521 ggatcgtttt ggcgttattg agcgttccgg ccaccagtac gcgctggcgg aacatctcgc
    26581 tggcaaagtt atagccgatt tcgttatcaa caaactcaat cgccatcaac atccctttac
    26641 cacgcgcttc ctgtaccaga tcgggatatt cccgcgccag ttgacggaaa ccgtccagca
    26701 acatatcgcc tttttgctca gcctgagccg gtaagttctg ctccagcaac acattgatgg
    26761 tcgccagcgc cgccgcacag gccagcgggt tgccgccaaa ggtggtggta tgcaggaatg
    26821 ggttgtcgaa cagaactgaa aacacctctt cagtggcgat ggtcgcgcca atcggcatca
    26881 cgccgccgcc gagggctttt gccagacaca gaatgtccgg ctgaacgttc tcatgttcgc
    26941 aggcaaacat cttgcccgtg cgccccatgc ccgtttgtac ttcatcgagg atcatcagtg
    27001 cgccgaactc atcgcacagc ttacgcacgg cggtgagata gcccggcggc ggcagaatta
    27061 cgccaccttc gccctgaatc ggttcgagga tcaccgcagc cacatcatca ccggtttttt
    27121 tgcactcatt aagagccgtg cgcatggctt cgatattgcc aaacggcaca tgacggaacc
    27181 ctggcagcag tggcataaac ggtttgcgga aggtcgattt cgccgtggcc gacagcgcgc
    27241 caagtgattt accgtggaac gcgccgctgg tggcaataaa agtaaatttg ccgcgcggcg
    27301 actgataagc cttcgccagc ttcagcgccg cttcaacgga ctcggtgccg ctattacaga
    27361 agaagctgta tttcagttta ccgggcgtta gcgcagcaag ggttttcgcc agcatagccc
    27421 gtagcggatc gagtagctcc tggctgtgca gtggttgttt cgcaagttga ttctgtacgg
    27481 cggaaaccac aactggatta cggtgcccca cgttgaaaat tccaaaacct cccaggcagt
    27541 cgataaactc ctgtccttgg gtgtcgacaa gcgtatttaa acctcccgct tgccactcta
    27601 cggctccgta atccccgccg gcggtaacag atttgcgata ctctaaaaac cccggattga
    27661 catgctcttt gaagtattca atcacctctc ggttaagtgc tttcatctcc tcatgatcca
    27721 gcgttcgctt ctcaatgaga ttcagggcgt gggcgctgca cgctaaagcc gatgcgctcg
    27781 aaggtaacct gttcaaaata tgctccggag gctcgcgtat cacatgatac cgatttaagt
    27841 attgcaggga ttgcgccact ccggctgcga tcgcgaaaat cgggataaac acaaatgcgg
    27901 ttatcatttg tgcaatattt aatgctatcc gttaacattt gttgttattt tgcacggttt
    27961 tgtcgggtat ggatttgatg attgctgcgc aaatgcacgt tttcagggca ataattgcct
    28021 gatttttggg caagataatt aacgttttcg tatagtaaca gagtgaaagt attttaaatc
    28081 aaagaattag atttgtgctt taatcgcaaa ttgcgatcta aatcaaatta atcggttaaa
    28141 gataaccgca gcggggccga cataaactct gacaagaagt taacaaccat ataacctgca
    28201 caggacgcga acatgtcttc tcatccgtat gtcacccagc aaaatacgcc gctggcggac
    28261 gataccactc tgatgtccac taccgatctg caaagctata tcactcatgc taatgacact
    28321 tttgtgcagg tgagcggctt taccttgcaa gagttacaag ggcagccgca caacatggtg
    28381 cgtcacccgg atatgccaaa agcggcgttt gcggatatgt ggttcaccct gaaaaaaggg
    28441 gagccctgga gcggcatcgt gaaaaatcgc cgcaaaaatg gcgaccatta ttgggtgcgg
    28501 gccaatgcgg taccgatggt gcgcgaggga aaaatcagtg gctatatgtc gattcgtacc
    28561 cgggcgacgg atgaagagat tgcggcggtg gagccgctgt acaaagcgtt gaactccgga
    28621 cgtaccagta agcgtattca taaaggcctg gtggtgcgta aaggctggct gggtaaactg
    28681 ccttcattac cgcttcgctg gcggacgcgt ggagtgatga ccctgatgtt tatcttgctg
    28741 gcggccatgc tttggtttgt tgctgccccg gtggtgacgt atttcctctg tgtgttagtg
    28801 gtattgttgg caagcgcttg ttttgaatgg cagattgtgc gcccgataga aaatgtcgcc
    28861 cgtcaggcac tgaaggtggc gaccggagag cgtaatagtg ttgagcacct gaatcgcagc
    28921 gatgagctgg ggctgacatt acgcgcggta gggcagcttg gcctgatgtg ccgttggcta
    28981 attaacgatg tctcaagcca ggtgtccagt gtcagaaatg gcagtgagac gctggcgaaa
    29041 ggcaccgatg aactgaacga acatacccag cagacagttg ataacgttca gcaaacggtg
    29101 gcgaccatga accaaatggc ggcgtcggtg aaacagaact ctgccacggc gtcggctgcc
    29161 gataaacttt ctatcaccgc cagtaatgcg gcagtgcagg gtggggaggc gatgaccacg
    29221 gtgatcaaga caatggacga tatcgccgac agtacccagc gcattggcac cattacttcg
    29281 ctgattaacg atattgcgtt tcagaccaat attctggccc tgaatgcggc ggtggaagcg
    29341 gcgcgtgccg gcgaacaggg caaaggtttt gcagtggtgg caggggaagt gcgtcattta
    29401 gccagccgca gcgctaatgc tgccaacgat attcgcaagc tgattgatgc cagtgctgat
    29461 aaggtgcaat ccggttcgca gcaggtacac gccgccggac ggacgatgga agatattgtg
    29521 gcacaggtga aaaacgtcac ccagttgatc gcccagatta gccattcaac gctggaacag
    29581 gccgatgggc tttccagcct gacccgtgca gtggatgagc ttaacctgat cacccagaaa
    29641 aatgccgagc tggtggaaga gagtgcgcag gtgtcggcga tggtgaaaca ccgcgccagc
    29701 cgactggaag acgcggtgac ggtgctgcat taatcggtat gccggatccg gcggttggag
    29761 cacaatgcct gatgcgatgc tgacgcatct tatcaggcct gcggggtgtg tagcgggtgt
    29821 aggccggata aggcgcgtca agcgtcgcat ccggcaattg caccgcgcca ctggcggatg
    29881 cggcgattaa tccagttgcg taatatcaaa agcggcgcgg tcgatcaccg caatgatctt
    29941 ttttatttgt gcatcactga tatcgctctg gttgacgcgt aaatccagca ccgctttaaa
    30001 attatccagc gcccgcttca tttgcgggtt ctggcgcagc gccgcgccaa cgcaacgcgc
    30061 tttgatgcgt tcttcaatca tctccacctg ttcgcggttt tcttccagcc actgcgcgcc
    30121 ttgttcggtc agcgcaatct gcttcttacc accttcctct tcgcggatgg taatcagcga
    30181 ctgctcctgc agaaaatcca gcgtcgggta gatgacgccc gggcttgggg tgtaattccc
    30241 ctgggttaga ttctcaatcg ctttaatcaa ttcgtaaccg tggctgtcat cgcgcgagag
    30301 aatatccaga atcaccagac gcaattcacc gtgaccaaag aaacgttgcc gacgaccgcc
    30361 gccgcgaccg tggcgaccac cgcaacattg accgtgttca tgctggtgac cgtgtccgca
    30421 gtgctcgtgt tctgacttct cacctttgca gcagccctca tggcgtggct ggccttcatg
    30481 tttacaacac ccttcgtgat gatggctcat atcagcctcc ttttgcttga ttagatatat
    30541 ctaatttata tctgatgtga gcattactgc aagtacaaaa atgattttta aacgtggaag
    30601 cacctcacat ctaggccgga taagacgttc acgccgcatc cggcagcatt gctatggcac
    30661 tgaatgcctt cacatacttc agacatatca gttctacaaa tcgcttgcat ttatcatgat
    30721 taacaatcat tatcatttgc gaattttatt tagatatatc tgattaccat cacgaaggcg
    30781 ataacaatga ataacacccc ccgctacccg cagcgcgttc gcaatgatct gcgcttccgt
    30841 gaactgactg tgctacgcgt tgagcgcatc agcgccggtt ttcagcgcat tgtccttggc
    30901 ggcgaggcgt tggacggttt tacatcgcgc ggctttgacg atcacagcaa actcttcttt
    30961 cctcaacctg acgctcactt tgtgccgcca acggtaacgg aagagggcat cgtctggccg
    31021 gaaggaccac gcccaccgtc gcgtgactat acgccactgt atgacgaact acgccatgaa
    31081 ctggcgatcg atttctttat tcacgacggt ggggtcgcca gcggctgggc gataaactta
    31141 cggtggcagg tccgcgcggt tcgctggtgg tgccggaaga ttacgcgtat cagctgtatg
    31201 tctgcgatga atccggaatg cccgcactgc gccgccgcct ggaaacgttg agcaaacttg
    31261 ccgttaaacc gcaagttagt gcgctggtta gcgtgcggga taacgcctgt caggattatc
    31321 tcgcgcacct tgatggtttt aatatcgaat ggctggcaca tgatgagcag gcggtagatg
    31381 cgcgtctggc gcagatgcaa atccctgcca gatgattact tcatctggat aaccggcgaa
    31441 ggtaaagtcg ttaagaattt aagccgccgc tttgaagcgg aacagtatga cccacagcgg
    31501 gtacgtgcag cggcttactg gcacgcaaag taactgatat ataaaaagaa aaaggctgac
    31561 gatttctcgt cagcctttgc tatatctggt ggcccctgct ggacttgaac cagcgaccaa
    31621 gcgattatga gtcgcctgct ctaaccactg agctaagggg ccatggtagc ggattataaa
    31681 gtaactccgt gtcgcaatcc agccattacc gcgcgcctgc tgtttttata aacaatgtat
    31741 tttcaatccg ttatactttt cctggtgatg ttagaaagga gtaaatatgg ttgaggatat
    31801 tttggctcca gggttacggg tcgtgttttg cggtatcaac cctgggcttt catccgccgg
    31861 gactggtttt ccctttgctc atccggcaaa tcgcttctgg aaggtgatat atcaggccgg
    31921 gtttaccgac cgtcagttga agccgcagga ggcacagcat ctgctggatt atcgttgtgg
    31981 cgtcaccaaa ctggtagacc gtccaacggt gcaagccaat gaagtttcaa agcaggagct
    32041 gcacgcaggt gggcgtaagc tgattgaaaa aattgaagat tatcagccgc aggcgttggc
    32101 gattctgggc aaacaagcat atgaacaggg attcagccag cgcggtgcac agtgggggaa
    32161 acaaacgctc accattggtt cgacgcagat ttgggtgctg ccaaatccca gcggtttaag
    32221 tcgcgtttca ctggagaaac tggttgaagc gtatcgcgag ctggaccagg cgctggtagt
    32281 gcgtgggcga taaaaaacgc caccga
//
LOCUS       DADMGC010000036        30364 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.036, whole genome shotgun sequence.
ACCESSION   DADMGC010000036 DADMGC010000000
VERSION     DADMGC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.036"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..378
                     /locus_tag="JNZ73_001609"
     CDS             <1..378
                     /locus_tag="JNZ73_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY8731927.1"
                     /translation="RAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILS
                     KDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDD
                     GLRFAIREGGRTVGAGVVAKVLS"
     gene            608..991
                     /gene="secE"
                     /locus_tag="JNZ73_001610"
     CDS             608..991
                     /gene="secE"
                     /locus_tag="JNZ73_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAY8731928.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            993..1538
                     /gene="nusG"
                     /locus_tag="JNZ73_001611"
     CDS             993..1538
                     /gene="nusG"
                     /locus_tag="JNZ73_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAY8731929.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1697..2125
                     /gene="rplK"
                     /locus_tag="JNZ73_001612"
     CDS             1697..2125
                     /gene="rplK"
                     /locus_tag="JNZ73_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAY8731930.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2129..2833
                     /gene="rplA"
                     /locus_tag="JNZ73_001613"
     CDS             2129..2833
                     /gene="rplA"
                     /locus_tag="JNZ73_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAY8731931.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3246..3743
                     /gene="rplJ"
                     /locus_tag="JNZ73_001614"
     CDS             3246..3743
                     /gene="rplJ"
                     /locus_tag="JNZ73_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAY8731932.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3810..4175
                     /gene="rplL"
                     /locus_tag="JNZ73_001615"
     CDS             3810..4175
                     /gene="rplL"
                     /locus_tag="JNZ73_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAY8731933.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4495..8523
                     /gene="rpoB"
                     /locus_tag="JNZ73_001616"
     CDS             4495..8523
                     /gene="rpoB"
                     /locus_tag="JNZ73_001616"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAY8731934.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EGGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGELVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8600..12823
                     /gene="rpoC"
                     /locus_tag="JNZ73_001617"
     CDS             8600..12823
                     /gene="rpoC"
                     /locus_tag="JNZ73_001617"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAY8731935.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13065..14198)
                     /gene="thiH"
                     /locus_tag="JNZ73_001618"
     CDS             complement(13065..14198)
                     /gene="thiH"
                     /locus_tag="JNZ73_001618"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAY8731936.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANNCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14195..15040)
                     /gene="thiG"
                     /locus_tag="JNZ73_001619"
     CDS             complement(14195..15040)
                     /gene="thiG"
                     /locus_tag="JNZ73_001619"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAY8731937.1"
                     /translation="MSSGRNISCRMATRSCFFRLLQGVEMLRIVDKTFDSHLFTGTGK
                     FASSQLMVEAIRASGSQLVTLAMKRVDLRQHNDAILEPLIAAGVALLPNTSGAKTAEE
                     AIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAETLVQQGFVVLPYCGADPV
                     LCKRLEEVGCAAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHAAQA
                     LEMGADAVLVNTAIAVADDPVNMAKAFRLAVEAGLLARQSGPGSRSYFAHATSPLTGF
                     LEASA"
     gene            complement(14967..15167)
                     /gene="thiS"
                     /locus_tag="JNZ73_001620"
     CDS             complement(14967..15167)
                     /gene="thiS"
                     /locus_tag="JNZ73_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAY8731938.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15151..15906)
                     /gene="thiF"
                     /locus_tag="JNZ73_001621"
     CDS             complement(15151..15906)
                     /gene="thiF"
                     /locus_tag="JNZ73_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HAY8731939.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTADINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15899..16534)
                     /gene="thiE"
                     /locus_tag="JNZ73_001622"
     CDS             complement(15899..16534)
                     /gene="thiE"
                     /locus_tag="JNZ73_001622"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY8731940.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16534..18429)
                     /gene="thiC"
                     /locus_tag="JNZ73_001623"
     CDS             complement(16534..18429)
                     /gene="thiC"
                     /locus_tag="JNZ73_001623"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAY8731941.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIHRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18510..18610)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18663..19139)
                     /gene="rsd"
                     /locus_tag="JNZ73_001624"
     CDS             complement(18663..19139)
                     /gene="rsd"
                     /locus_tag="JNZ73_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HAY8731942.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19234..20007
                     /gene="nudC"
                     /locus_tag="JNZ73_001625"
     CDS             19234..20007
                     /gene="nudC"
                     /locus_tag="JNZ73_001625"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HAY8731943.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20047..21111
                     /gene="hemE"
                     /locus_tag="JNZ73_001626"
     CDS             20047..21111
                     /gene="hemE"
                     /locus_tag="JNZ73_001626"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HAY8731944.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21268..21939
                     /gene="nfi"
                     /locus_tag="JNZ73_001627"
     CDS             21268..21939
                     /gene="nfi"
                     /locus_tag="JNZ73_001627"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HAY8731945.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21982..22572
                     /locus_tag="JNZ73_001628"
     CDS             21982..22572
                     /locus_tag="JNZ73_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HAY8731946.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22759..23031
                     /gene="hupA"
                     /locus_tag="JNZ73_001629"
     CDS             22759..23031
                     /gene="hupA"
                     /locus_tag="JNZ73_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HAY8731947.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23044..23739
                     /locus_tag="JNZ73_001630"
     CDS             23044..23739
                     /locus_tag="JNZ73_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HAY8731948.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRASFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23741..24166)
                     /gene="zraP"
                     /locus_tag="JNZ73_001631"
     CDS             complement(23741..24166)
                     /gene="zraP"
                     /locus_tag="JNZ73_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HAY8731949.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24404..25772
                     /gene="zraS"
                     /locus_tag="JNZ73_001632"
                     /pseudo
     CDS             24404..25772
                     /gene="zraS"
                     /locus_tag="JNZ73_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
     gene            25769..27094
                     /gene="zraR"
                     /locus_tag="JNZ73_001633"
     CDS             25769..27094
                     /gene="zraR"
                     /locus_tag="JNZ73_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HAY8731950.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27091..28380)
                     /gene="purD"
                     /locus_tag="JNZ73_001634"
     CDS             complement(27091..28380)
                     /gene="purD"
                     /locus_tag="JNZ73_001634"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAY8731951.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28392..29981)
                     /gene="purH"
                     /locus_tag="JNZ73_001635"
     CDS             complement(28392..29981)
                     /gene="purH"
                     /locus_tag="JNZ73_001635"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAY8731952.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7111 a         7912 c         8355 g         6986 t
ORIGIN      
        1 cgtgctggtg agaacgtagg tgttctgctg cgtggtatca aacgtgaaga aatcgaacgt
       61 ggtcaggtac tggctaagcc gggcaccatc aagccgcaca ccaagttcga atctgaagtg
      121 tacattctgt ccaaagatga aggcggtcgt catactccgt tcttcaaagg ctaccgtccg
      181 cagttctact tccgtactac tgacgtgact ggtaccatcg aactgccgga aggcgtagag
      241 atggtaatgc cgggcgacaa catcaaaatg gttgttaccc tgatccaccc gatcgcgatg
      301 gacgacggtc tgcgtttcgc aatccgtgaa ggcggccgta ccgttggcgc gggcgttgta
      361 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
      421 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      481 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      541 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      601 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      661 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      721 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      781 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      841 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      901 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      961 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
     1021 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
     1081 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
     1141 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
     1201 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
     1261 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
     1321 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1381 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1441 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1501 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1561 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1621 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1681 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1741 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1801 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1861 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1921 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1981 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     2041 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     2101 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     2161 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     2221 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     2281 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     2341 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2401 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2461 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2521 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2581 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2641 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2701 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2761 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2821 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2881 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2941 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     3001 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     3061 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     3121 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     3181 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     3241 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     3301 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3361 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3421 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3481 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3541 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3601 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3661 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3721 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3781 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3841 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3901 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3961 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     4021 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     4081 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     4141 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     4201 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     4261 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     4321 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4381 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4441 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4501 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4561 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4621 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4681 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4741 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4801 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4861 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4921 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4981 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     5041 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     5101 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     5161 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     5221 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     5281 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     5341 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5401 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5461 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5521 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5581 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5641 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5701 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5761 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5821 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5881 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5941 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     6001 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     6061 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     6121 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     6181 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     6241 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     6301 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6361 ctggatgaag gaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6421 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6481 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6541 gcgaacatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6601 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6661 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6721 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6781 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac tggttgaacg tggcgacgtg
     6841 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6901 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6961 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     7021 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     7081 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     7141 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     7201 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     7261 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     7321 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7381 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7441 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7501 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7561 cacgagttcg agaagaaact cgaagcgaaa cgccgaaaaa tcacccaggg cgacgatctg
     7621 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7681 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7741 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7801 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7861 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7921 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7981 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     8041 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     8101 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     8161 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     8221 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     8281 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     8341 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8401 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8461 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8521 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8581 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8641 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8701 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8761 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8821 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8881 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8941 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     9001 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     9061 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     9121 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     9181 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaactaagcg
     9241 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     9301 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9361 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9421 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9481 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9541 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9601 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9661 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9721 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9781 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9841 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9901 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9961 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
    10021 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
    10081 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
    10141 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
    10201 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
    10261 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
    10321 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10381 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10441 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10501 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10561 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10621 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10681 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10741 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10801 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10861 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10921 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10981 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    11041 tggtacccat gaaggtatca tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc
    11101 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    11161 tgatatcctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    11221 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    11281 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    11341 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11401 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11461 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11521 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11581 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11641 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11701 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11761 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11821 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11881 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11941 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    12001 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagaaccggc
    12061 aatcttggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    12121 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    12181 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    12241 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    12301 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12361 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12421 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12481 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12541 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12601 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12661 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12721 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12781 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12841 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12901 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12961 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    13021 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    13081 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    13141 gcggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    13201 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    13261 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    13321 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13381 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13441 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13501 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13561 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13621 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13681 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13741 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13801 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13861 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13921 ccgcagtacg tgcagttgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13981 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    14041 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    14101 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    14161 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    14221 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    14281 gcaggcctgc ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    14341 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgcggcat
    14401 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14461 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14521 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14581 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14641 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14701 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14761 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14821 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14881 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14941 acgttttgtc cacaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    15001 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    15061 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    15121 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    15181 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    15241 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    15301 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15361 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15421 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15481 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15541 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15601 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15661 cgggctgttg atatctgcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15721 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15781 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15841 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15901 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15961 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    16021 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    16081 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    16141 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    16201 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    16261 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    16321 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16381 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16441 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16501 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16561 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16621 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16681 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16741 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16801 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16861 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16921 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16981 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    17041 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggtg
    17101 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    17161 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    17221 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    17281 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    17341 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17401 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17461 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17521 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17581 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17641 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17701 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17761 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17821 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17881 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17941 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    18001 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    18061 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    18121 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    18181 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    18241 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    18301 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18361 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18421 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18481 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18541 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18601 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18661 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18721 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18781 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18841 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18901 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18961 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    19021 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    19081 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    19141 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    19201 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    19261 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    19321 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19381 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19441 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19501 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19561 accgaatggg cgatgctgtg cagccattgc cgtgagcgtt actacccgca aatcgccccc
    19621 tgcattattg ttgccatccg tcgcgatgat tcgatcctcc tcgcccagca tacccgccat
    19681 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19741 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19801 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19861 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19921 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19981 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    20041 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    20101 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    20161 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    20221 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    20281 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    20341 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20401 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20461 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20521 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20581 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20641 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20701 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20761 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20821 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20881 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20941 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    21001 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    21061 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    21121 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    21181 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    21241 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    21301 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21361 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21421 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21481 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21541 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21601 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21661 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21721 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21781 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21841 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21901 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21961 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    22021 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    22081 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    22141 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    22201 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    22261 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    22321 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22381 caggctggtc gcgaaatgag cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22441 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22501 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22561 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22621 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22681 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22741 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22801 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22861 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22921 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22981 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    23041 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    23101 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    23161 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    23221 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    23281 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    23341 ccgccggtga acattcgcgc gagttttgac gatcgcggcg atctgagctt tatgcaacgt
    23401 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23461 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23521 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23581 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23641 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23701 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23761 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23821 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23881 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23941 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    24001 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    24061 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    24121 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    24181 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    24241 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    24301 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24361 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24421 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24481 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24541 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24601 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24661 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24721 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24781 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24841 ttatcgccag tttcaaccga tgtttgctgc tggaatgcac cggatgcgcc atatgcagca
    24901 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24961 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    25021 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    25081 agatgaaatg aagcgcaaag agaagcttgg ggcatcttgc ggcaggcgtt gcccacgaaa
    25141 tccgtaaccc actttcctcg attaaaggac tggcgaaata ctttgccgag cgcgcgcctg
    25201 cagggggaga agcgcatcaa ctggcgcagg tgatggcgaa agaggccgac cgtttaaacc
    25261 gcgtggtaag cgagttgctg gaactggtta agccaacgca tctggctttg caggcggtgg
    25321 atctcaacac gctgattaac cactcattac agctggtaag ccaggatgca aacagccggg
    25381 agatccagtt acgctttacc gccaacgaca cattaccgga aattcaggcc gatccggaca
    25441 ggctgactca ggtcctgttg aatctctatc tcaatgctat tcaggcgatt ggtcagcatg
    25501 gcgtgattag cgtgacggcc agcgaaagcg gcgcgggcgt gaaaatcagc gttaccgaca
    25561 gcggtaaggg aattgcggca gatcagcttg aagccatctt cactccgtac ttcaccacca
    25621 aagccgaagg caccggattg gggctggcgg tcgtgcataa tattgttgaa caacacggtg
    25681 gtacaattca ggtcgcaagc caggagggaa aaggctcaac gttcaccctc tggcttccgg
    25741 tcaatattac gcgtaaggac ccacaaggat gacgcacgat aatatcgata ttctggtggt
    25801 ggatgatgac attagccact gcactatttt gcaggcttta ctgcgcggct ggggctataa
    25861 cgtcgcgctg gcgaacagcg ggcgacaggc gctggagcag gtgcgggaac aggtttttga
    25921 tcttgtgctt tgcgatgtgc gaatggcgga gatggacggc atcgccacgc tgaaagagat
    25981 caaagcgtta aacccggcaa ttccggtgct gattatgact gcgtactcca gcgtcgagac
    26041 ggcggtagag gcactgaaaa ctggggcgct ggattatctc atcaagccgc tggatttcga
    26101 taacctacag gcgacgctgg aaaaggcgct cgcgcatacg cacagtattg atgctgaaac
    26161 acctgcggtg actgccagcc agttcggtat ggtcggtaaa agcccggcga tgcaacacct
    26221 gctcagtgaa atcgccctcg tcgcgccatc ggaagccacg gtgctgatcc acggcgattc
    26281 cggcaccggt aaagagctgg tcgccagggc gattcacgcc agtagcgcac gtagcgaaaa
    26341 accgctggta acgctcaact gtgcggcact caacgaatcc ttgctggaat ctgaattgtt
    26401 cggtcacgaa aaaggggcgt ttactggagc cgataaacgc cgggaagggc gctttgttga
    26461 ggcggacggc ggcacgctgt ttctcgatga aattggcgat atctcgccga tgatgcaggt
    26521 gcgtctgctg cgtgcgattc aggagcgcga agttcagcgt gtcggtagca accagactat
    26581 ctcggttgat gtccggctga ttgcggcgac tcatcgcgat cttgccgcag aggtgaatgc
    26641 cgggcgtttt cgccaggatc tctactatcg cctgaatgtg gtggcgattg aagtaccatc
    26701 gctgcggcaa cggcgggaag atatccctct gctggctggc cattttcttc agcgctttgc
    26761 cgagcgtaat cgcaaggcgg taaaaggctt tacgccacag gcgatggatt tgctgatcca
    26821 ctacgactgg ccgggaaata ttcgtgaact ggaaaacgcg gtggaacggg cggtggtgct
    26881 gctgaccggg gaatatattt ccgaacgcga gctgccgctg gcgattgcca gtacgccgat
    26941 cccgctgggg caaagtcagg atattcagcc gctggtggaa gtggaaaaag aggtgattct
    27001 ggcggcgctg gagaaaacgg gcggcaacaa aaccgaagcc gcccgtcagt tagggatcac
    27061 gcgcaaaacg ctattggcaa aactgtcgcg ttagttctgc tcgcgttcga tggcgcgcca
    27121 accgatatct ttccggcaga agcaatcatc ccagtggata tcggtcatta aggcgtaggc
    27181 gcgtttctgc gcttctgcca cggtatgacc cagcgcggtg acgcacagta cgcgcccgcc
    27241 gctggttacc acctgctcgt catccgccag ttttgtgccc gcgtggaaca ctttgccgtc
    27301 agccacttct tccagcggca ggccgtggat cacatcgccg gtgcggtaat cgcccggata
    27361 tccacccgca gccatcacca cgctgagaga agcacgttca tcccactctg acgttttctc
    27421 gtccagtttg ccttcacagg ccgccaggca gagctcaacc agatcggact tcatgcgcag
    27481 cataatcggc tgggtttccg gatcgccaaa gcggcagtta aattcaataa ccttcggatt
    27541 gccctgtttg tcgatcatca ggcccgcata gagaaaaccg gtgtaggtgt tgccttccgc
    27601 cgccatgcct ttcacggttg gccagatgat acgttccatg gtgcgctgat gaacttcatc
    27661 ggttactacc ggcgcggggg agtaagcgcc catcccgccg gtgtttggtc cgttatcttt
    27721 atcgcctacg cgtttgtgat cctggctggt agccatcggc agcacatgct cgccgtccac
    27781 catcacgata aagctcgctt cttcgccatc gaggaactct tcgataacga tgcgatgacc
    27841 cgcgtcgcca aaagcgttgc ccgccagcat atcgtgaaca gccgcttccg cttcttccag
    27901 cgtcatcgcc acgataacgc ctttcccggc agccagaccg tccgctttaa tgacgattgg
    27961 cgcgcctttc tcacgcagat acaccagcgc aggttctacc tcggtgaagt tctggtattc
    28021 cgccgtaggg atcttatggc gggccaggaa atctttggta aacgcttttg agccttccag
    28081 ttgggccgca cctgcggttg ggccgaagat tttcagcccg gcggcgcgga aggtatcgac
    28141 cacgcctttc accagcggcg cttccgggcc gacgatggtc agatcaatct tttcgttttg
    28201 tgcgaaatcc aacagcgccg ggatatcggt cacgccgata gcaacgtttt gcagcgcggg
    28261 ttccagcgca gtgcctgcat tacccggagc aacaaaaaca gtctcaacca gcggcgactg
    28321 ggccgctttc caggccagcg cgtgctcgcg cccgccgtta ccaatcacta atactttcat
    28381 ctattgctcc attaatggcg gaagtggcgc atgtcggtga agagcatcgc aataccgtgc
    28441 tcgtcggcgg cggcaatcac ttcgtcatca cggatggaac cgccaggctg gattacgcag
    28501 gtcacgcccg cagcggcggc ggcatcaata ccgtcgcgga acgggaagaa cgcgtcagaa
    28561 gccatcgagg aacctttcac ttccaggcct tcatcggcag ctttaatacc ggcgattttc
    28621 gcggagtaca cgcggctcat ctggcccgcg ccaataccga tggtcatatt gtttttggca
    28681 tagacgatag cattggattt cacgaatttc gccactttcc agcagaacag cgcatcacgc
    28741 agttcctgtt cggtcggctg acgttgggtg acgacgcgca gttcttccgc accgaccatc
    28801 cccaggtcac gatcctgaac cagcagaccg ccgttcacgc gtttgaaatc aagacccgga
    28861 acacgctcgc cccactgacc gcaggtcaga acgcgtacgt tctgcttggc ggcggtgatt
    28921 ttcagggctt cttcgctggc ggaaggcgca ataatcactt cgacaaactg acgagaaatg
    28981 atggcctgtg cggtttccgc atccagctcg cggttaaagg caatgatgcc gccgaatgcg
    29041 gaggttgggt cggttttgta cgcgcgatcg taagcatcaa gaatggaatt gccgatagct
    29101 acaccgcaag ggttggcgtg cttcacaatc acacatgccg gctcggcgaa ctctttcacg
    29161 cactccagcg ccgcatcggt atcggcgatg ttgttataag agagggcttt accctgaacc
    29221 tgggttgcgg tagcaacgga ggcttctttc acattctctt ctatatagaa ggcagcctgc
    29281 tggtggctgt tctcgccgta acgcatatcc tgcttcttaa tgaagttcag gttcagcgtg
    29341 cgtgggaagc gaccggcggc ttctttgctt tcaccgtggt acgccggaac catgctgccg
    29401 aagtagttgg caatcatgct gtcgtaggcg gcagtgtgtt cgaaggcttt gatggcgagg
    29461 tcgaaacggg ttgcaagcgt cagcgatcct tcgttgtcat ccatctcttt aataatggcg
    29521 tcgtagtcgc tgctcttcac cacgattgcg acatctttat ggttcttagc ggcggagcgc
    29581 accatggtcg ggccgccgat atcgatgttc tcaaccgcat cttccagcga gcagccttca
    29641 cgagcaacag tctgggcgaa cggatacagg ttaacaacca ccatatcgat aggctggatc
    29701 tgatgttctt ccataatggc atcgtcctgg ccgcgacggc ccagaatgcc accatgtact
    29761 ttcggatgca gggtcttcac gcgtccatcc atcatctccg ggaaaccggt gtaatcggaa
    29821 acttcggtta ccggcagacc tttttctgct aacagacggg cagtgccccc tgtagacagc
    29881 agctccacac cgcgtgcgga aagtgcctgg gcgaattcga cgataccggc tttgtcagaa
    29941 acactgagca gagcgcggcg gactggacga cgttgttgca tggtaaatcc cttgggtttg
    30001 actattacag agagcgttag ctgaattttt cgcgaaaaac tcagctaacg cccctaacgg
    30061 ggcatcctta tttttcgccc gcattgtaac gaaaacgttt gcgcaacgct cgcgaatttt
    30121 tctctttcaa tggtgatcac aattttgaca gtggttaccg tgggaaaaat acagaaatta
    30181 cattgatgat tgtggataac tctgtgcgta aaaaggtata aagcggggtt ttgctgggga
    30241 atgcagcagt cagtcatttt tctgcaattt ttctattgcg gcctgcggag aactccctat
    30301 aatgcgcctt catcgacacg gcggatgtga atcacttcac acaaacagcc ggttcggttg
    30361 aaga
//
LOCUS       DADMGC010000037        29934 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.037, whole genome shotgun sequence.
ACCESSION   DADMGC010000037 DADMGC010000000
VERSION     DADMGC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.037"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            229..1677
                     /locus_tag="JNZ73_001636"
     CDS             229..1677
                     /locus_tag="JNZ73_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAY8731953.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1679..1804
                     /locus_tag="JNZ73_001637"
     CDS             1679..1804
                     /locus_tag="JNZ73_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731954.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1801..1872)
                     /locus_tag="JNZ73_001638"
     CDS             complement(1801..1872)
                     /locus_tag="JNZ73_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731955.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            1927..2475
                     /gene="idi"
                     /locus_tag="JNZ73_001639"
     CDS             1927..2475
                     /gene="idi"
                     /locus_tag="JNZ73_001639"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAY8731956.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            complement(2518..4035)
                     /gene="lysS"
                     /locus_tag="JNZ73_001640"
     CDS             complement(2518..4035)
                     /gene="lysS"
                     /locus_tag="JNZ73_001640"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAY8731957.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4045..5143)
                     /gene="prfB"
                     /locus_tag="JNZ73_001641"
     CDS             complement(join(4045..5067,5069..5143))
                     /gene="prfB"
                     /locus_tag="JNZ73_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAY8731958.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5234..6967)
                     /gene="recJ"
                     /locus_tag="JNZ73_001642"
     CDS             complement(5234..6967)
                     /gene="recJ"
                     /locus_tag="JNZ73_001642"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAY8731959.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(6973..7683)
                     /gene="dsbC"
                     /locus_tag="JNZ73_001643"
     CDS             complement(6973..7683)
                     /gene="dsbC"
                     /locus_tag="JNZ73_001643"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HAY8731960.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            complement(7708..8604)
                     /gene="xerD"
                     /locus_tag="JNZ73_001644"
     CDS             complement(7708..8604)
                     /gene="xerD"
                     /locus_tag="JNZ73_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAY8731961.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8716..9237
                     /gene="fldB"
                     /locus_tag="JNZ73_001645"
     CDS             8716..9237
                     /gene="fldB"
                     /locus_tag="JNZ73_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HAY8731962.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9277..9684)
                     /locus_tag="JNZ73_001646"
     CDS             complement(9277..9684)
                     /locus_tag="JNZ73_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731963.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9665..9931)
                     /gene="sdhE"
                     /locus_tag="JNZ73_001647"
     CDS             complement(9665..9931)
                     /gene="sdhE"
                     /locus_tag="JNZ73_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HAY8731964.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            10174..11154
                     /gene="ygfZ"
                     /locus_tag="JNZ73_001648"
     CDS             10174..11154
                     /gene="ygfZ"
                     /locus_tag="JNZ73_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HAY8731965.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11350..12009)
                     /locus_tag="JNZ73_001649"
     CDS             complement(11350..12009)
                     /locus_tag="JNZ73_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAY8731966.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12173..12484)
                     /locus_tag="JNZ73_001650"
     CDS             complement(12173..12484)
                     /locus_tag="JNZ73_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAY8731967.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLPELKKVIADIYPGQTQFYMIEFKC
                     L"
     gene            12529..13961
                     /locus_tag="JNZ73_001651"
                     /pseudo
     CDS             12529..13961
                     /locus_tag="JNZ73_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
     gene            complement(14018..14761)
                     /locus_tag="JNZ73_001652"
     CDS             complement(14018..14761)
                     /locus_tag="JNZ73_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8731968.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGRSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15028..17901)
                     /gene="gcvP"
                     /locus_tag="JNZ73_001653"
     CDS             complement(15028..17901)
                     /gene="gcvP"
                     /locus_tag="JNZ73_001653"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HAY8731969.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEEIGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18020..18409)
                     /gene="gcvH"
                     /locus_tag="JNZ73_001654"
     CDS             complement(18020..18409)
                     /gene="gcvH"
                     /locus_tag="JNZ73_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAY8731970.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18433..19527)
                     /gene="gcvT"
                     /locus_tag="JNZ73_001655"
     CDS             complement(18433..19527)
                     /gene="gcvT"
                     /locus_tag="JNZ73_001655"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HAY8731971.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLIVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            19818..19901
                     /locus_tag="JNZ73_001656"
     CDS             19818..19901
                     /locus_tag="JNZ73_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731972.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            complement(19975..21177)
                     /gene="ubiI"
                     /locus_tag="JNZ73_001657"
                     /gene_synonym="visC"
     CDS             complement(19975..21177)
                     /gene="ubiI"
                     /locus_tag="JNZ73_001657"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HAY8731973.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLSEWLR"
     gene            complement(21200..22378)
                     /gene="ubiH"
                     /locus_tag="JNZ73_001658"
                     /gene_synonym="visB"
     CDS             complement(21200..22378)
                     /gene="ubiH"
                     /locus_tag="JNZ73_001658"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HAY8731974.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22375..23700)
                     /gene="pepP"
                     /locus_tag="JNZ73_001659"
     CDS             complement(22375..23700)
                     /gene="pepP"
                     /locus_tag="JNZ73_001659"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAY8731975.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23726..24304)
                     /gene="ygfB"
                     /locus_tag="JNZ73_001660"
     CDS             complement(23726..24304)
                     /gene="ygfB"
                     /locus_tag="JNZ73_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HAY8731976.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24472..24801
                     /gene="zapA"
                     /locus_tag="JNZ73_001661"
     CDS             24472..24801
                     /gene="zapA"
                     /locus_tag="JNZ73_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAY8731977.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            24843..25026
                     /gene="ssrS"
                     /locus_tag="JNZ73_001662"
     ncRNA           24843..25026
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="JNZ73_001662"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25047..25649
                     /locus_tag="JNZ73_001663"
     CDS             25047..25649
                     /locus_tag="JNZ73_001663"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAY8731978.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25746..25805)
                     /locus_tag="JNZ73_001664"
     CDS             complement(25746..25805)
                     /locus_tag="JNZ73_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY8731979.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26138..26197)
                     /gene="ibsC"
                     /locus_tag="JNZ73_001665"
     CDS             complement(26138..26197)
                     /gene="ibsC"
                     /locus_tag="JNZ73_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HAY8731980.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26426..27658)
                     /locus_tag="JNZ73_001666"
     CDS             complement(26426..27658)
                     /locus_tag="JNZ73_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAY8731981.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(27914..28573)
                     /gene="rpiA"
                     /locus_tag="JNZ73_001667"
     CDS             complement(27914..28573)
                     /gene="rpiA"
                     /locus_tag="JNZ73_001667"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAY8731982.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            28956..29849
                     /locus_tag="JNZ73_001668"
     CDS             28956..29849
                     /locus_tag="JNZ73_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HAY8731983.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
BASE COUNT         7113 a         8063 c         7504 g         7254 t
ORIGIN      
        1 atgccagaat gattaatgaa atatcaatat gagaaaatta cagataattc cattgtcagg
       61 ttctcttgtc attttcgtgg tttaagcacg attatgtgat tgcctccgca attcctgtct
      121 ctaactcccc ttcctcgcaa aaactggcac tccacgagca tgtgtttaga cagtttcatt
      181 aacgtaaacg gttgcttttt attctggcgg gcgaaaggag aaacactgat gagcgccata
      241 gattcccaac ttccctcatc ttctgggcaa gaccgcccaa ctgatgaggt tgaccgcata
      301 ttatcaccag gaaagctgat catactcggt ctgcaacacg tccttgtcat gtacgcaggt
      361 gcagtcgctg ttcctcttat gattggtgac cgactgggcc tctcaaaaga tgctattgcg
      421 atgctcatta gctcggatct cttttgctgc gggatcgtca cattattgca atgtatcggt
      481 atcggccgct ttatggggat ccgcctgccg gtgattatgt cggtgacctt tgctgctgta
      541 acaccaatga tagccattgg gatgaacccg gatatcggcc tgctggggat atttggtgcc
      601 actatcgccg cgggttttat caccacatta ttagcgccac ttattggtcg cttgatgcct
      661 ttattcccgc cactggttac cggtgtggtt attacttcta ttgggcttag catcattcag
      721 gtgggtattg actgggccgc tggaggtaaa gggaatccgc aatatggtaa tcccgtttat
      781 ttaggtatct cctttgccgt cttaattttt atcttgctca ttactcgcta tgcgaaagga
      841 tttatgtcca acgtcgccgt attactgggg attgtatttg gctttttact ttcgtggatg
      901 atgaatgaag tcaatttatc cgggctacat gatgcctcgt ggtttgcgat tgtcaccccg
      961 atgtcgtttg gtatgccgat tttcgatccc gtttccattc tgaccatgac tgccgtgtta
     1021 atcatcgtgt ttatcgagtc gatggggatg ttcctggcac tgggtgaaat agtcggtcgt
     1081 aaactctctt cacacgatat tattcgcggg ctgcgtgtcg atggcgtagg gacaatgata
     1141 ggcggcacgt ttaacagctt cccccacacg tcattttctc aaaacgttgg cctggttagc
     1201 gtgatgcgcg ttcatagccg ctgggtgtgt atttcttcgg gaattatatt aatcctgttt
     1261 ggcatggtgc caaaaatggc ggtgctggta gcctccattc cgcaatttgt gctgggcggc
     1321 gctggtctag tgatgttcgg catggtactg gcgacaggga ttcgaattct gtcgcgctgt
     1381 aactacacca ccaaccgtta caacctctat attgtggcga tcagtctcgg cgttggcatg
     1441 actccgacgc tctctcacga tttcttttct aagttaccgg ccgtactgca accgctgcta
     1501 catagcggca ttatgctcgc aacccttagc gccgttgtgc tgaacgtctt ctttaatggc
     1561 tatcagcatc atgctgacct ggtgaaggaa tccgtctctg ataaagattt aaaagtcagg
     1621 acagtacgta tgtggcttct gatgcgcaag ctgaagaaaa atgagcatgg agaataatat
     1681 gaatttttta atgcgcgcta tattcagtct gttgttgctt tttactctct ctattcctgt
     1741 catttctgac tgtgttgcaa tggccattga aagtcgcttc aaatatatga tgctactttt
     1801 ttaaatggtt tttacctgtc ggcatccgct caaaacgggc ggttgtcgat aaacgctctc
     1861 ttggttaatc atttcactct tcaattatct ataatgatga gtgatcagaa ttacatgtga
     1921 gaaattatgc aaacggaaca cgtcatttta ttgaatgcac agggagttcc cacgggtacg
     1981 ctggaaaagt atgccgcaca cacggcagac accctcttac atctcgcgtt ctccagttgg
     2041 ctgtttaatg ccaaggggca attattagtt acccgccgcg cactgagcaa aaaagcgtgg
     2101 cctggcgtgt ggactaactc ggtttgtggg cacccacaac tgggagaaag cagcgaagac
     2161 gcggtgatcc gccgttgccg ttatgagctt ggcgtggaaa ttacgcctcc tgaatctatc
     2221 tatcctgact ttcgctaccg cgccaccgat ccgagtggca ttgtggaaaa tgaagtgtgt
     2281 ccggtatttg ccgcacgcac caccagtgcg ttacagatca acgatgatga agtgatggat
     2341 tatcaatggt gtgatttagc tgatgtatta cgcggtattg atgccacgcc gtgggcgttc
     2401 agtccgtgga tggtgatgca ggcgacaaat cgcgaagcca gaatacgatt atctgcattt
     2461 acccagctta aataaaaaac cccgacattt gccggggttg tgagcataac gtaatgctta
     2521 tttcaccgga cgcatcgccg ggaacagaat aacgtcgcgg atggtatggc tgttggtgaa
     2581 cagcattacc atacggtcga tgccaatccc cagacctgct gtcggcggta agccatgttc
     2641 cagtgcggta acgtaatctt catcgtagaa catcgcttcg tcgtcacctg cgtctttcgc
     2701 ggcaacctga tccaggaagc gttgcgcctg atcttccgcg tcattcagct cgctaaagcc
     2761 gttaccgatt tcacgaccac cgatgaagaa ctcaaagcgg tctgtgattt ccgggttaat
     2821 gtcgttacga cgcgccagcg gagaaacttc tgccggatat tcagtaatga aggtcggctg
     2881 aatcagatgt gcttctgcca cttcttcgaa gatctcggta acgatacggc ccagacccca
     2941 gctcttctca acgtggatac cgatagattc agcaatcgct ttcgcagagt cgaagttatc
     3001 cagatccgcc atgtcggttt ccgggcgata tttcttgatc gcttcacgca tggtcagttt
     3061 ttcgaacggt ttaccgaagt ccagcgtcac gtcgccgtag gtcacttccg tcttaccgag
     3121 aatatcctgt gccagagtac ggaacagcga ttcggtcagc tcgatcagat ctttgtaatc
     3181 tgcgtaagcc atgtagagtt ccatcatggt gaactctggg ttatgacgta cggaaatacc
     3241 ttcgttacgg aagttacggt tgatttcgaa tacacgctcg aagccaccga ccaccagacg
     3301 cttgaggtac agttccggcg cgatacgcag gtacatgtcg agatccagcg cgttatggtg
     3361 ggtgataaac ggacgcgcag cggcaccgcc tgggatcacc tgcatcatcg gcgtttcaac
     3421 ttccataaag ccacggttca ccatgaactg gcgaatacca gagaggatct gcgagcgtac
     3481 tttaaaggtg ttacgggatt catcgttaga gatgagatcc agataacgct gacgatagcg
     3541 cgcttcctga tcctgcaagc cgtggaattt atccggcagc ggacgcagtg ctttggtcag
     3601 cagacgcagc tcggtacagt ggatagacag ttcgccggtt ttggttttga acagtttacc
     3661 tttcgcgccg aggatgtcgc cgaggtccca ttttttgaac tgctcgttgt aaacgccttc
     3721 cggcagatcg tcacgggcaa cgtacagctg aatgcggcca cccacatcct gcagggtaac
     3781 gaaagacgct ttacccataa tacggcgggt catcatgcgg ccagcaacgg cgacttcgat
     3841 gttcagcgct tccagttctt cgttctcttt accgtcgaat tctgcgtgca attggtcaga
     3901 ggtatgatcg cgacggaaat cgttcgggaa ggcaattccc tgctcacgca ggttcgccag
     3961 cttctcacga cgcgttttca gttcattgtt aagatcgact accgcgtcag cgccctgtgc
     4021 gtgttgttca gacatgttgg ttcctcataa ccctgctttc aaacttgctt cgataaattg
     4081 atccaggctg ccgtccagca cggcctgcgt gttgcgagtt tctaccccgg tgcgcagatc
     4141 tttaatgcgg gagtcatcaa ggacataaga acgaatctgg ctgccccagc cgatgtcaga
     4201 tttgttatct tccatcgcct gtttctcggc atttttcttc tgcatctcca gttcataaag
     4261 cttcgctttc atctgcttca tggcctgatc tttgttcttg tgctgggaac ggtcgttctg
     4321 gcactgggtc acgatcccgg tcgggatgtg ggtaatacgc accgcagatt cggtacggtt
     4381 aacgtgctga ccgcccgcgc cggacgcgcg ataaacgtca atgcgcagat ccgccgggtt
     4441 gatttcgata tcaatatcat catcaacttc cggataaaca aacgcagagc tgaacgacgt
     4501 gtggcgacga ccgccggagt caaacgggct tttacgcacc aggcggtgaa cgcccgtttc
     4561 tgtacgcagc cagccgtaag cgtaatcgcc ggagattttg atcgtcacgg atttaatacc
     4621 cgccacttca ccttccgact cttcgatgat ttcagttttg aaaccacgcg attctgccca
     4681 gcgcagatac atacgctcaa gcatgctcgc ccagtcctgt gcttccgtac cgccagaccc
     4741 cgcctgaata tcgaggtaac agtcggcgct gtcatattcg ccagagaaca tacggcggaa
     4801 ctcaagctgc gccagttttt cttccagggc gtcgagttca gcaacggctt cgttaaaggt
     4861 ttcttcgtcg tcagcttcta cagccagttc cagcagacca gaaacatctt ccagcccctg
     4921 tttcatttgg tcgagggtgt cgacaacggc ttcgagggag gaacgctctt tacccagcgc
     4981 ctgtgcgcgt tcgggttcgt tccagacatc cggctgttcc agctcggcgt ttacttcttc
     5041 cagacgctct ttcttggcgt cgtagtcaaa gataccccct aagaacgtcg gagcgttccg
     5101 tgaggtcctg aatgcgatta tttaccggat taatttcaaa catggtctga tttcttttat
     5161 tgagctagtc aaaatgcggt gataagagcg ggattgtacc caatccacgc tcttttttat
     5221 agagaagatg acgctaaatt ggccagatat tgtcgatgat aatttgcagg ctgcggttgc
     5281 cgcgaaactc gttgatatcg agcttataag ccagttgcac ttcgcggacg ccgttatccg
     5341 gccagagagc ggtatcgaca ttaaaagcaa taccatccag cagtggaccg ccgccgaccg
     5401 gttcgaccat caccttcaaa tgacgttcgc ccaccagccg ctgttgcagc agacggaaat
     5461 gaccgtcaaa cagcggctcc gggaacatct gcccccacgg gccagcatcg cgcagcagct
     5521 gcgccacttc catggtcatt tcggccgggc ttaacggacc gtctgacacc acttcgcctt
     5581 gcaatagcga aggggccagc cactcagtaa ccagttcgcc aaaccgttgt tgaaagagtt
     5641 cgaatttatc ctcttccagc gacaaacccg ccgccatcgc atgaccgcca aacttgagca
     5701 tcatgccggg gtagagtgtg tctaatcgct ccagcgcatc acgcatatgc agcccctgaa
     5761 tggagcgacc ggaacctttc agcgtaccgt cacctgctgg cgcaaaggcg ataaccggac
     5821 ggtgaaaacg ctctttgata cgcgaagcca gaataccgac aacgccctga tgccattcgg
     5881 ggtgatacat tgccagcccg ccgggtagcg tgtcacggct gcgctccagt ttctcgcaca
     5941 gggtcagggc ttcaacctgc attccttgtt cgatctcttt tcgcgtctgg tttagcgcat
     6001 cgagttcatt tgccagcacg cgcgcttcgc cgatgttgtc gcacagcaac agcgccacac
     6061 caacggacat atcatccagt cgcccggcag cattgagacg cggtcccagc gcaaaaccta
     6121 aatcgctggc ggctagtttt tgtgcatcgc gatttgccac ttccagcagc gctttaatcc
     6181 ctggacggca ctttccggca cggatgcgac tcatcccctg ccaggtcaga atgcgattat
     6241 tagcgtccag cggcacgacg tccgctactg tccccagcgc taccagatcc agcagttctg
     6301 ccaggttagg aattgcgatg ccgcgctcat caaaccagcc ctgatcgcgc aaaaaggtgc
     6361 gcagcgccag catcagataa aacgccacac ccacgcctgc cagtgatttc gacgggaaat
     6421 tacagtcgcg caagttaggg ttaatgatcg cttccgctgc gggtaatgtg tcgcccggca
     6481 aatggtgatc ggtaacaata accgggatgc ccaacgagcg agcgtgctca actcccgcat
     6541 gggaggaaat accgttatcc accgtgacaa ttaactgcgc gccacgggca tgggcctgat
     6601 cgaccacttc cgggcttaag ccgtaaccgt cttcgaaacg gtttggtacc aggtagtcga
     6661 tattgctgca accaagcgag cgcatcgcca gcacgcttag agccgtgctg gtcgcgccgt
     6721 cggcgtcgaa atcaccgacc acaataatcc gcgttccttc gcgaaaagcg ttgtaaagga
     6781 tctcaacggc cttttcgaca ccgcttagtt gctgccaggg caacatacct ttaacactgc
     6841 gttccagttc ttgcgcactg cgtactcccc ggctggcgta taaacggcgc agcaagggag
     6901 gcaattcagc gggcaagtct gccgtttcat cgacttcacg gcgacgaagt tgtatctgtt
     6961 gtttcacgcg aattatttac cgctggtcat tttttggtgt tcgtcgagga attctttcat
     7021 gtctttcggc ggctggtaac ccggaacaag tgtgccattg ctcagcacaa ctgccggagt
     7081 accgctaacg ccaagctgga cgccaagtgc gtaatggtcg gcaatatcca cgtcgcagct
     7141 ggctggtgcg acgcttttac ctgccatcac atcatcaaac gctttgtttt tatctttcgc
     7201 acaccagata gctttcattt ctttctctgc atcgctgtcc agcccctggc gcgggaaagc
     7261 aagataacgc acggtgatcc ccagcgcgtt atagtctgcc atttgctcat gcagtttgtg
     7321 gcagtaacca caggtaatat cagtaaacac ggtgatgacg tgtttttcct gcggcgcttt
     7381 ataaacgatc atctcttttt caagcgcatt caactgcttt aacagcatct tattggtgac
     7441 attgaccgga gccgtgccac taacgtcata cattggcccc tgaatgatat gtttaccatc
     7501 atcggtgatg tacaacacac cgctgttagt cagaactgtc ttcatgccag ctacgggcgc
     7561 gggctgaata tcgctgcttt tgatgcccat tttggctaac gtttgttgaa ttgccgcgtc
     7621 atcagcctga gcaaagcctg aaaacgccgc taacaaagta aacaacataa aacctttctt
     7681 cataaatctt cccgttcttt tcagacatca cgcccgcggg tgatgctgtt gatgaagttg
     7741 ccgcagacgc tcggtagcga catgcgtata aatttgcgtg gtggaaagat cgctgtggcc
     7801 cagtagcatc tgcaccacgc gtaaatccgc accatgattt aataaatgag tggcaaaagc
     7861 gtgacgcaac acatgcggtg acagcttttc gctgtcgata cccgccagca cggcataatg
     7921 tttaatgcgg tgccagaagg tctgtcgcgt catctgctgc gcacgctggc tgggaaacaa
     7981 cacgtcaatt gacacaccat tcagcagcca cggacgccca tgttccagat aggtttccag
     8041 ccagtaaacc gcctcttccc ctaacggcac caggcgctct ttgttgcctt taccaatgac
     8101 ccgcactacg ccctgacgca ggctgatatc actcattgtc agcccgacca gttcagagac
     8161 acgcagcccg gtagcataca acacttcaag catggcttta tcgcgtagct ccagtggctg
     8221 atcaattaat ggcgcctgta ataaacgttc gacctgcgct tcgcttaaat cttttggcaa
     8281 acgctggggc aatttcggtg aagcgagatg cgcactggga tcgtcttcac gaaacttttc
     8341 gcgataaaga tactggaaca atcggcgcac tgcactcagc aaacgcgctg agctggtggc
     8401 tttatacccg ccctccagcc gttctgccag taatgcctgc aaatcgtcac tttgcgccgt
     8461 cgccagcgtc aacccgcggt gatgcaacca ctccaccatc attgacagat cgcgacggta
     8521 agcgttcaac gtattttcag ccagattttt ttccagccac agagcatcaa gaaactgctc
     8581 gatgcgtgcc agatcctgtt tcacttgcgc cccttatggt cactcatttg atccattatg
     8641 ccttattgtg ccgtgactaa agcgattctg atacactagc tgcaaaagcc acagcagaat
     8701 cgagaagctt acgttatgaa tatgggtctt ttttacggtt ccagcacctg ttacaccgaa
     8761 atggcggcag aaaaaatccg cgatattatc ggcccagaac tggtgacctt acataacctc
     8821 aaggacgact ccccgaaatt aatggagcag tacgatgtgc tcattctggg tatcccgacc
     8881 tgggattttg gtgaaatcca agaagactgg gaagccgtct gggatcagct cgacgacctg
     8941 aaccttgaag gtaaaattat tgcgctgtat gggcttggcg atcaactggg atacggcgag
     9001 tggttcctcg atgcgctcgg tatgctgcat gacaaactct cgaccaaagg cgtgaagttc
     9061 gtcggctact ggccaacgga aggatatgaa tttaccagcc cgaaaccggt gattgctgac
     9121 gggcaactgt tcgtgggtct ggcgctggat gaaactaacc agtatgacct tagcgacgag
     9181 cgtattcaga gctggtgcga gcaaatcctc aacgaaatgg cagagcatta cgcctgatct
     9241 cactgacggc ttagcgcata tgctttgccg tcatctttat ctttgcgtct cttgttgcaa
     9301 caaaatccgc cgtaaatccc gccattcggc ttcgtccatg ctgtcggctg ccagccataa
     9361 atgttgccgt ttaccgccat cagaacgtaa acgcagcatc atgccgctct taatcatcca
     9421 cggtgctttg acgatgctcc actcctgccc ttgccaacgc aaacgcccgt ccatcaacaa
     9481 gcgaatttcc ccctggcgag cattaataca ccgctggctg cgaacgcaat caaataccac
     9541 cagcgaaagt aacaccatcc ataacggggt gtaactgagc ggccagggca tgagtaaaat
     9601 aacagcggca accagcccat gaatcagcaa ggaaagccac tgtgcgcgcc aggagacgcg
     9661 caaatcagat tgccacagga ccacgttccc ggttccgtgt ctggatgagt cggaccatta
     9721 tttccagttc tgcatcggcc ggtttaccgt gattcatcag ccagttaaac aggtccggat
     9781 cgtcacattc cagcagacga ataaagatgc gtttttcgtc atcgcttaag ctgtcgtact
     9841 catgttcgaa aaacggcatg attgaaatat cgagttcgcg cataccacgg cggcatgccc
     9901 aatgaatgcg ggctttgttg ttaatgtcca tcttcttcct gtctcacgaa aatccagtac
     9961 ccggctattg taacgtgttt ttcgacttct tttacgggaa tatcagtaaa caccatcgcg
    10021 atcgcgaaat taattcacaa caattcaagg gcttcatttt tttggcagtc gcctcgcaga
    10081 aggcacagat cgcgtagtga aagcacttgc attgcctcat agctctttta ccattagtca
    10141 ttaatacgcc gttaagcaac tcaggactct attatggctt ttacaccttt tcctccacgt
    10201 cagcctacgg cttctgcccg tttaccgctg acgctgatga cgcttgatga ctgggcgctt
    10261 gccaccatta ctggcgcgga cagcgaaaaa tatatgcagg gtcaggtgac agcagatgtc
    10321 agccagatgg cagaagatca gcacctgctc gccgcccatt gcgacgccaa aggtaaaatg
    10381 tggagcaatt tacgtctgtt ccgcgacggc gatggctttg catggattga acggcgcagc
    10441 gtgcgtgaac cgcagctgac tgaactgaaa aaatatgcgg tattctctaa agtgaccatc
    10501 gcgccagacg acgagcgtgt gctgcttggt gttgccggtt ttcaggcgcg cgccgcgctg
    10561 gcaaatctct ttagcgaact gccttcgaaa gaaaaacagg tagtcaaaga aggcgcgacc
    10621 actttgctat ggtttgaaca cccggcagaa cgtttcctga tcgtaaccga tgaagctact
    10681 gctaatatgc tgaccgataa actgcgcggt gaagcggaac tgaacaatag ccaacagtgg
    10741 cttgcattaa acattgaagc tggtttcccg gtgattgatg ccgccaacag cgggcagttt
    10801 atcccacagg cgaccaatct ccaggcgctg ggcggtatca gctttaagaa aggctgttat
    10861 accggacaag agatggtggc gcgagcaaaa ttccgtggtg ccaacaaacg cgcgctctgg
    10921 ttgctggcag gtagtgccag ccgactgccg gaagctggtg aagacttaga gctgaaaatg
    10981 ggcgagaact ggcgtcgtac cggtacggtg ctggctgcgg taaaactgga agatggtcag
    11041 gtcgtggtac aggtcgtcat gaataacgat atggaaccgg acagcatctt ccgcgtacgc
    11101 gacgatgcga atacactgca tatcgagccg ctgccgtatt cgctcgaaga gtaaatctct
    11161 gtatcgcatc aggcatttat cgcctgatgc gacgctgacg cgtcttatca tgtctgggat
    11221 ttgatgcctt tatcacaaca gggacgaggc ccgtagatcg gataaggcgt tcacgccgca
    11281 tccggcggtt gcgctccgat gcctgatgcg acgctgacgc gtcttatcag gcctacaaag
    11341 gcatacccat tacgcctgcc caatatacaa atagatcgcc agaaagtggc acacactacc
    11401 gccgagcacg aagccgtgcc agatggcatg gttgtatgga atgcgtttgc agacgtagaa
    11461 aatcaccccg agcgaataaa ccacgccgcc taccgccagt aaggtaacgc tgcccgccgc
    11521 gagcttaact gccatttcat aaattaccac cagcgacagc cagcccatcg ccagataagt
    11581 caccagagat aaaattttga atcggtgcgc gatggtcagt ttgaacagaa tccccagcaa
    11641 tgctaggctc cagataacaa tcatcaaccc gcgcgctaac ggagaatcca gccccaccag
    11701 caaaaacggc gtgtaggttc cggcaatcaa caggtaaata gcgcaatggt caaatttctt
    11761 cagccacatt tttgcccgtt gatgaggaat ggcgtgatag agcgtcgaag cgaggaacag
    11821 caggatcata ctgccgccat agaggctgta actggttatc gctgtggcgc tggcattgag
    11881 atccaccgcc tgaaccagta gcaacaccag cccaacgata ccaaacacca gcccaatgcc
    11941 gtgactgacg ctgttggcaa tttcctctgc cagcgaatat ccctgcttaa tgaggggctt
    12001 ctgaaccata acttactcca gagaaacgta cacgcacatg tatacctctc cagcgtaact
    12061 gagaatagtt ccagtgaaca cctgttagct aaaataaatt ttgatttgag actaatctcc
    12121 taaaaatcat gaaattaaat gcgaaatttc aactaacagg cgtcagttca atttaaagac
    12181 atttaaattc aatcatataa aattgtgtct gaccgggata gatgtcagca atgacctttt
    12241 tcagttcagg cagggtcata ttttcctgct ctgcatgttt ttccgtcagt gtatccagcg
    12301 ttacggttga ggttgcggtg acttcaatcg tgcaaaaata accgtcatct tcaaaacgtc
    12361 cgacacgaag cacatcaccc gttttgaagt gcgattcaga ctcgtcgcgg atggtgatgg
    12421 ttttacgccc agccagaatg tcatcctgga aacgttgaaa aaaagtgatg tcgtttggct
    12481 gcatggtact atttcctgta agaattgact gatctggagc ctatgatagt gaaaaaactc
    12541 accttaccga aagatttctt atggggtggc gccgttgccg ctcatcaggt cgaaggcggc
    12601 tggaacaaag gcggcaaagg accgagcatt tgtgacgttc tgaccggtgg cgcacacggc
    12661 gtgccgcgcg aaatcaccaa agaagtcttg ccaggaaaat actatccaaa ccatgaagcc
    12721 gttgattttt atggtcacta caaggaagac atcaagctat ttgccgaaat gggcttcaaa
    12781 tgttttcgga cttccatcgc ctggacgcgt atttttccaa aaggtgatga agctcagcca
    12841 aacgaagaag ggctgaagtt ttacgactct ctgttcgatg aactgctgaa atacaacatc
    12901 gaaccggtga taaccctctc ccactttgaa atgccgctgc atctggtgca gcaatacggt
    12961 agctggacca accgtaaatt cgttgatttc tttgtccgtt tcgcggaagt ggtattcgaa
    13021 cgctacaagc ataaagtcaa atactggatg accttcaacg aaataaacaa ccagcgtaac
    13081 tggcgtgcgc cgctgtttgg ttactgctgc tccggcgtgg tgtataccga gcatgacaac
    13141 ccggaagaga cgatgtacca ggtgctgcat caccagtttg tcgccagcgc cctggcagtg
    13201 aaagccgcgc gtcgcattaa cccggagatg aaagtcggct gtatgctggc gatggtgccg
    13261 ctctatccct actcatgtaa cccggacgat gtgatgttcg ctcaggagtc gatgcgcgaa
    13321 cgctacgtct ttaccgatgt gcagctatgc ggctattacc cgtcctatgt attgaacgag
    13381 tgggaacgtc gcggatttaa tatcaaaatg gaagacggcg atctcgatgt actgcgcgaa
    13441 ggcacctgcg attatcttgg tttcagctat tacatgacca acgcagtgaa ggccgaaggc
    13501 ggcaccggcg atgcgatctc tggttttgaa ggcagcgtac cgaacccgta tgttaaagcg
    13561 tctgactggg gctgcagatt gatccagtag gtctgcgcta tgccctttgc gaactgtatg
    13621 agcgttatca gaggccgctg tttattgtcg aaaacggttt tggcgcttac gacaaagtgg
    13681 aagaagatgg cagcatcaac gacgactacc gcattgacta cctgcgcgcc catattgaag
    13741 agatgaaaaa agcagtgact tacgatggtg tggatctgat gggctacaca ccgtggggct
    13801 gcatcgactg cgtgtcattc actaccgggc agtacagcaa acgctacggc tttatctatg
    13861 tgaataaaca tgacgacggt actggcgata tgtcgcgttc acgtaagaag agctttaact
    13921 ggtacaaaga ggtgattgcc agcaacggcg agaagcttta agtcgatgaa gtaccggatg
    13981 caatacttgt tgcatccggt cagacaactt ccctgtttta tttcccgccc gccaaatcga
    14041 taaaacttcc cgtgacgtaa gaggctttat cacttagtag ccagacaatg gcctgcgcga
    14101 cctcttctgc ctgtccacca cgctgcatgg ggatgttcga cttaacgcga tcgacgcgtc
    14161 caggctcgcc gccgctggcg tgcatttcgg tataaataaa ccctggccgc acgcagttaa
    14221 cgcggatccc ctgcgcggcg acttccagcg atcgtccggt ggttaacgta tcaatcgccc
    14281 ctttcgatgc cgcgtaatca acatattccc ctggcgaacc caaccgtgag gccaccgaag
    14341 agacattgac gatagcgccg ccactgccac catttttaag cgccatgcgt tttaccgcct
    14401 cgcggcagca gagaaaatat cccgtcacgt tggtggaaag tactcggttg attcgctctg
    14461 cggtaaggtt ttcaacggtg cactgggtaa acaagatccc ggcgttattg accagcgctg
    14521 ctagcggttc atcgtgctga tcgattgctg taaacatcgc aacgacctgg ttttcgtcgc
    14581 tgatatccgc ctggagcacg aatgctttgc caccggcttg cattattaag ttcatcactt
    14641 cctgcgccgc gtggaggttt tgctgataat taaccgccac cgtataccct tcttgcgcca
    14701 acagtaatgc agttgcccgc ccgatgccgc gactgccacc ggtcacaagt gctatagcca
    14761 tttgtttttc cgagaaaaat taattcgttg acgtatcttt gagattgcta acaaactggc
    14821 tcaatcttca atgttggcac gcttttcccc ctcgcccttt cagggagatg gtcggggtga
    14881 gggtaaatat tcgcgccagt gccggcctgt tcccctcacc ctaaccctct ccccaaaggg
    14941 gcgaggggac tgtctgagca cttttgtact ttgtcatctg actaaaaagg cgccgaagcg
    15001 cctttagaaa atactcgaat cagtgaatta ctggtattcg ctaatcggta cgcaggagca
    15061 gaacaggtta cggtcgccgt aaacatcatc cagacgtttc accgtcggcc agtatttgtc
    15121 tgccacgcct gccgggaata ccgcaacttc acggctgtac ggatgcgccc actcggcgac
    15181 cagttcgctc tgaatgtgcg gcgcgttcac cagcgggtta tcttccagcg gccagacacc
    15241 ggctttcacc tggtcaattt ctgcgcggat agccagcatc gcgtcgataa agcgatccag
    15301 ttccactttg ctttcagatt cagtcggttc aaccatcagc gtacccgcca ccgggaacga
    15361 catcgtcggc gcgtggaaac cgtagtcgat caggcgcttg gcaatatcca gctcgctgat
    15421 gccgatttct tctttcagcg ggcgaatatc gagaatacat tcgtgcgcca cgcgaccgtc
    15481 gcgaccggta tacagcaccg ggaaggcatc ctgcaggcgg ctggcaatat agttggcgtt
    15541 gaggattgcc acctggcttg cttttttcag cccttctgcg cccatcatgc ggatgtacat
    15601 ccagctgatt ggcaggatag aggcgctacc gaacggtgcc gcagaaactg cgccctgacg
    15661 ggttaacatg ccttcgattt gcaccacgct atgacccggt acaaacggtg ccagatgcgc
    15721 tttcacaccg atcggtccca tacccggacc accaccgccg tgcggaatgc agaaagtttt
    15781 atgcaggttg aggtgcgaaa cgtccgcgcc aataaagccc ggcgaggtga tgccaacctg
    15841 ggcgttcatg ttcgcgccat caaggtaaac ctgaccgccg aactgatgca cgacttcaca
    15901 cacttcacgg atcgtttctt catacacgcc gtgggtagaa ggataagtca ccatgataca
    15961 ggagaggtta tcgcccgcct gttccgcttt cgcgcgcaga tcagtcagat cgatgttgcc
    16021 gtttttatca cacgccacaa ccaccacctg cattcctgcc atatgtgcag aagcggggtt
    16081 agttccgtgc gcagaagccg ggatcaggca gatatcgcga tgcccttcgt tgcggctttc
    16141 atgataatga cgaatcgcca gcaggcccgc gtattcgccc tgtgcgccgg agttcggctg
    16201 catacaaacg gcgtcgtaac cggtcagttt caccagccag tcagccagct gcgcaatcat
    16261 ctgctgataa ccttcagcct gttccggcgg gcaaaacggg tgcagttcgg caaattccgt
    16321 ccaagtgatt gggatcatct cggcggcggc gttcagtttc atggtgcagg aacccagcgg
    16381 gatcatcgcc tgattcagcg ccagatcttt acgctccagc gagtgcatat agcgcatcat
    16441 ttcggtttcg ctgtgatagc gattaaacac cggatgggtg aggatttcgt cgtcgcgcag
    16501 catcgcaggc tggatagagc ggctgtcgtg agccacgtct ttgtccagcg tgtcgatctc
    16561 cagcccgtgg ttatcgccca gcagcacact gaaaagctgc attacgtttt cacgcgtggt
    16621 ggtttcatca agggtgatcc ccacggcgtt caggatatcg ctacgcaggt tgatttcagc
    16681 cgcttcggca cgcgtcagta cgcccgcttt gtcggctact tccacacaca aggtgtcgaa
    16741 atagtgcgca tggcgcagtt tcagaccttt ttgttgcagg cccgccgcca gaatatcggt
    16801 cagacggtga atgcggttag cgatacgttt caggccaacc gggccgtgat aaacggcata
    16861 caggctggcg atgtttgcca gcagtacctg ggaagtacaa atgttggagt tcgctttctc
    16921 acgacgaata tgttgctcgc gagtctgcat cgccatgcgc agcgcggtat tgccagctgc
    16981 atctttcgat acaccgataa tacggcccgg cattgagcgt ttgtattcat ctttcgccgc
    17041 aaagaatgcc gcgtgtgggc caccgtagcc catcggcacg ccgaagcgtt gcgccgaacc
    17101 aaaaacaata tccgcgccct gtttacccgg cgcagttaac agcaccagcg ccataatatc
    17161 ggcggcaacg ctgaccacaa ttttgcgtga tttcagttcg ctaataagcg cagtgtagtc
    17221 gtgaatttca ccggtagtgc ctacctgctg taacagcacg ccgaagacgt cctgatggtc
    17281 gagcactttt tgcgcgtcat cgacaatcac ttcaaaacca aaggtttcgg cacgagtacg
    17341 gaccacatcc agtgtttgcg gatgcacatt ggaagccacg aagaagcggt tggcattttt
    17401 cagtttgctg acgcgtttcg ccatcgccat tgcttcggcg gcagcggtgg cctcgtccag
    17461 aagagaagca gaggccatat ccagtccagt caaatccagc gttacctgct ggaagttgag
    17521 cagtgcttca aggcggccct gggagacttc aggttgatac ggagtgtacg cggtatacca
    17581 gcccggattt tccagcatgt tacgcaggat aaccggcggt agctgcacgg cggtgtaacc
    17641 catgccgatg taagacgtga agcgtttatt gcgactggca atagccttga gttctgccag
    17701 tgcggcgtat tcggtcgccg gtgcgccaac ctgcggtggt gtcgcaagct gaatatcttt
    17761 cggcacaatc tggccggtca gcgcgtttaa cgattgtgca ccaacggcat tcagcatttc
    17821 ttgctgttgc gcggcgtccg gtccgatatg gcgttcaata aaagcgccgc tgttttcaag
    17881 ctggcttaac gtctgtgtca tgagcgatgg ttcctgaaac gtgcagtgaa ttgtgaacct
    17941 ctctccttac gaagagagtg agggtgaggc ataaattttc ctcaccctga tcctctcccg
    18001 cagaagagga ataaagccgt tactcatctt ctaacaatgc ttcgtatgcg gtcgcatcca
    18061 gcagtgattc cagttcgctt tcatcgctag ctttgatttt aaagatccag ccgcctgcat
    18121 acggttcgct gttcaccagt tccggggaat cgctcagtgc gtcgtttacc gccacgattt
    18181 caccgcttac tggcgcataa atgtctgacg ccgcttttac cgattcggca accgcgcagt
    18241 catcgcccgc gctaaccgtt gcgcccactt ccggcaggtc aacaaacacc atatcgccta
    18301 acagctcctg agcatgttcg gtaataccaa cggtgtaagt gccgtcggct tctttacgca
    18361 gccattcgtg ttctttgctg tatttcagtt ctgctggtac gttgctcatc aatcaatctc
    18421 caaaaaagta aatcacgcga cggctttgcc gttacgcaca aaaacaggtt ttgtcacttt
    18481 aaccggcatt tcacggttgc gaatttgcac aatcgccgtt tcgccaatac cttccggcac
    18541 gcgcgccagc gcaatgctgt aacccagcgt cggggagaaa gtaccgctgg tgataatgcc
    18601 ttcatgctgg ttgccctgcg catcggtaaa gcgtaccggc agttcattac gcagcacgcc
    18661 tttttcggtc atcaccagac caaccagttt ttctgtacca tgctcacgct gcacttccag
    18721 ggcttcacga ccgataaagt cacgatctgt cggttcccag gcgatagtcc agcccatgtt
    18781 ggcggctaaa ggagaaatag tttcgtccat ctcctgacca taaagattca tgcccgcttc
    18841 cagacgcagc gtgtcacgcg cccccagacc gcagggctta acgcccgctt ccaccagcgc
    18901 acgccagaaa tcggccgctt tttcattggg cagcgcaatt tcatagcccg cttcaccggt
    18961 ataaccagtg gtggcaataa acagatcgcc cacctgcacg ccaaagaacg gtttcatccc
    19021 ttccaccgcc tgacgctgag catcattaaa cagtgtggca gcttttgcct gagcattcgg
    19081 cccttgcacg gcaatcatgg aaaggtcatc acgaacggta atttcgatgc cgaaaggttc
    19141 agcgtgttgg gtaatccagg agaggtcttt ttcgcgggtg gcggagttaa caatgaggcg
    19201 gaagaaatct tcagtaaagt agtagacgat gagatcatct atcacaccgc cagaggcatt
    19261 caacatcccc gagtaaaggg ctttgccgct tttggtgagc ttcgccacat cgttcgccag
    19321 cagataacgc agaaactccc gggtgcggct gccgcggaga tcgacgatgg tcatatgcga
    19381 cacatcaaac attccggcat cggtacgtac cgcatgatgt tcgtcgattt gcgaaccgta
    19441 atgcagcggc atcatccagc cgtggaaatc caccatgcga gcgccgcaaa gcgtgtgttg
    19501 ttcgtacaaa ggagtctgtt gtgccatctt gtcctcattg aataagcggg gctgacaact
    19561 ttttcatggt gaaattatca ccacaaaacc cagcatcgga gccactcccg gtccccaacg
    19621 caatcgttct cttttgcctg aacttaccac cgaaacagac tgttaaccat aaggtaaaat
    19681 tgatcatcac attagcttat ggttaaaaaa tgcaaaaatc gcgacagaat aaaaaaccaa
    19741 aaaatacacc aatttctata caaagatgat gtgatgagaa agtcaatttg aataagacaa
    19801 tattaagagc taaaaaaatg tcaaaaaaca ctaaatcaaa aaatcatggc attagaaaat
    19861 ataatgcgaa aacggaggtg aaattagttt atttcaaatg aggaaaatct cccggcgaaa
    19921 aaaccgggag atgaaagtgt gatgggtatc aaataaacaa cagaggagaa atttttaacg
    19981 cagccattca gacaaatcgt ttaatcccat tgcctggcgg ataagttgcg gcttaacgcc
    20041 aggaagcgtg tcggccagtt tcaaaccaat atcacgcagc agttttttcg ccggattagc
    20101 gccggaaaag agatcgcgaa aaccctgcat accagccagc atcagcgccg cgctgtgctt
    20161 gcggctacgt tcatagcggc gcaggtaaat gtactgcccg atgtctttac cctgacgatg
    20221 caaccgtttc agttcagcaa tcagctctgc ggcgtccata aatccgagat taacgccctg
    20281 ccccgccagt gggtgaatag tatgcgcggc gtcgcccacc agcgccagac ggtgtgcggc
    20341 aaactggcgc gcataacgcc ccgtcagcgg gaacacctga cgcgcgctct caaccttgca
    20401 taagccaagg cgattatcaa aagcgatatt taacgcgcga ttaaattcgt cttcacttgc
    20461 ctgctgcatc cgctgcgctt cctctggcga cagtgaccag acaatcgagc aaagatgcgg
    20521 atcgctaagc ggtaaaaagg ccagaatgcc ttcgccatgg aaaacctgcc gcgccaccgc
    20581 atcatgcggt tcttccgtgc gaatggtcgc taccagcgcg tgatgctgat aatcccagaa
    20641 agtcagcgga atatctgctt tgttgcgcaa ccaggaatta gcaccgtccg cgccaatcac
    20701 cagacgcgcc gttaacatgc tgccatcttt cagcgtcagg aaggtttcat tttctcccca
    20761 ggcgacctgc tgtaattctg tgggggccaa cagagtaata tctgacgact gctgcgcttt
    20821 gttccacagc gcgtagtgaa tcactgagtt ttcaacgatg tgcccaagat ggctatagcc
    20881 catgctttga tcgtcaaacg agatgtgacc aaaactgtct ttgtcccaca cttccatgcc
    20941 gtgatagcaa ctggccctac gagcgagaat gtcctgccag acgccaagac gggtgagtaa
    21001 tttttcgctg gcggcattga tagccgaaac gcgcagttgt ggtagtgcat ctaccgccag
    21061 aggttccggt acgcgctgct ccagtacggc aacgcgtaag ccgctcccct gtaagccgca
    21121 ggcaaccgcc agccccacca tgccgccgcc aacaatggct acatcaacac tttgcattgt
    21181 ttattcctta aaaccgcctt caacgcgcca cccaaccgag ggtgcgctgc gccagcacat
    21241 cgcgtgccgg ggtgaataat tccatcgtca tcagtccgat gttgcgcccg acaaccagcg
    21301 gtgcccaacg gttggcaaaa agatgtacca ggctgtccgt gacgccgatg gttgcttcgc
    21361 gatcgctctg tcgacgctgc tgatagcggc acaatatgcc gtaatcaccg atgtcttctc
    21421 cgcgctcatg cgcctgagtc agggtttccg caaggctcat gacatctcgc ataccgaggt
    21481 taaacccttg cccggcaatc gggtgcagag tttgcgccgc attgcccacc agcacagtgc
    21541 gatgggtaat cggtctggcg gcgcgggtta atgccagcgg ataagcactg cgtttaccgg
    21601 cgtgggtaat ttgccccagt cgccagccaa aggcagactg gagttcacgg caaaacttct
    21661 cgtcgctcca cgacagcacc tcttcgcgcc gttccagtgg atgacaccag accagcgaac
    21721 agcgtccgtc agacatcggc aacatcgcca gcgggccatg ttgcgtgaag cgttcaaaag
    21781 cgcggccttc gtgcgcaaca gaagtagcaa cgttggcaat cactgccagt tgttcgtaag
    21841 gctcctgctg ccaatcaacg ccgcacgcgg tggctaacgc tgaatgggtg ccatcagcgg
    21901 ctatcagcac gcggcccgtc agcatctcgc cgctctccag cgtcacttca acgtgactct
    21961 gagtacgggc aacgttagct acgcgatcgg ggcaatgcag cattacgcca ggtgcttttc
    22021 gcagcaatgc aaacagccgt tgcccaacat tatgcaattc gacaacctgc cccagcgccg
    22081 ccagttggta atcttctgcg gcgagggtga cgaatccagc atgaccacga tcgctgacat
    22141 gcacggtagt gatggcggtt gcgcaatcag ccagagattg ccagacgccg atgcgcgcca
    22201 gttgctgaca ggtacccgcc gccagcgcta tcgctcgtcc atcaaagccc ggatgagcat
    22261 gtgactctgg cgcagtcgct tcaatcaaat gtaccggcag cgccccgtga cttaaccggg
    22321 aaatagccag cgccagcgtc gcgcccgcca tgccgccacc gacgatgatt acgctcattg
    22381 ctttctcgca gcagccatca acgcttcgat ttcttccggc tttttcacca cgctggcggt
    22441 gaggttttcg ttgccggttt cggtaatcac aatgtcgtct tcaatacgaa tgccgatacc
    22501 gcgatattgt tctggcacat ccgcatccgg cgcgatatac agaccaggct ctacggtcag
    22561 taccatcccc ggttccagaa tgcgcgagcg atcctgacca taaacgccaa cgtcatggac
    22621 atccagtcct aaccagtggc taaggccatg cataaagaaa ggacgatggg cgttctgagc
    22681 gatcagttca tcaacattac ctttcaggat gccgagtttt accaggccgc taaccatgat
    22741 gcgcaccact tcaccagtga cttccagaat ggaagttccc ggacgataca ggcgcaggct
    22801 ggtttcgaga gactccagca caatgtcgta gatttcacgc tgggcctggg tgaatttgcc
    22861 gttgaccggg aaggtgcggg taatatcgcc cgcgtaacct ttgtattcac aacccgcgtc
    22921 aatcaacacc aggtcaccgt cgcgcagttc actctcgttt tcggtgtagt gcagaatgca
    22981 gccgttttca ccgctgccta caatggtgtt ataggaagga tagcgcgcac cgtggcggtt
    23041 aaattcgtgg tgaatttcgc cttccagatg gtactcgaac attcccggac ggcatttttc
    23101 catcgcccgt gtatgtgcca tggcggtgat ttctcccgcg cggcggagta cggcaatctc
    23161 ttctggcgat ttgaacaggc gcatttcatg aacaacagga cgccagtcga tcatcgttgc
    23221 cggtgcggtg agattttgcc gcgaaccttt acgcagtttt tccagcgcac tgttcacgat
    23281 ttcatcagca tatgcatatt cgccctgggc atggtaaacc acatccaggc cgttaagtag
    23341 ttgataaagt tgctgattga tttcgctgaa tgccagtgcg cggtcaacgc ccagtttctc
    23401 tggcgcggca tcctggccta agcgacggcc aaaccagatc tccgccgtca ggtcgcgaac
    23461 gcggttaaac agaacgctgt ggttatgagt gtcatcgctt ttaatcagca ccagcaccgc
    23521 ttccggttcg ttaaagccgg tgaagtacca gaagtcactg ttctgacgat aggggtattc
    23581 gctgtcggcg ctacgtgtta cttctggtgc agcaaaaatc agcgcggcgc tgccgggttg
    23641 catttgctcc accagggcct gacggcgacg ctgaaactct tgccgggata tctcactcat
    23701 aacactctcc ttacgttttt tgtttttagt gtagagtcgg tttttgtact tctggcgcgg
    23761 tcggttgcgg atgagtaaag gtgtcgtggc ataacagcgc ggcaacacga acgtattcga
    23821 tgatctcttc aagcgacatt tcaagctctt cctgatcttc gtcttcgtcg tagcccagct
    23881 gcgcgatgtt acgcagatcg tcgatggctt caccggtttc gccggtcact ttgtccagct
    23941 tcggttgcgt aacgccaaga ccaagcagga agtgattgac ccaacctgcc agtgcatcag
    24001 cccgatcgaa aacgctgaca tcatcgccat caggcagata aagctgaaaa aggaagccgt
    24061 catcctgcag ggcatcgctg gtggcagagt gcattttacg cagtgcctgt gccagctcat
    24121 gaccgaaagc catgccttcg ttcgtcaggt cgtgaagtag cggcagccat gagctgtcat
    24181 cgttaccgcc acatatcatc ccactgatta aaccatgcat ctcggcaggg gtcagacccg
    24241 tcccttgttg gttcagatac tggttcattt cgttgtaacc aggcatttcg ttctgtatag
    24301 acataagcat tcgtcgtcaa agggaggaat attcatgata tgctaccact ttgggccctg
    24361 gtggaccaga aaagggcttg tctcttctca tcagggtagc tatagcgtcg ccccttcgca
    24421 gaccatgggt ctaaagacga aggcagcgca gtcaatcagc aggaaggtgg catgtctgca
    24481 caacccgtcg atatccaaat ttttggccgt tcactgcgtg tgaactgccc gcctgaccaa
    24541 agggatgcgt tgaatcaggc agcggacgat ctgaaccaac ggttgcaaga tctgaaagaa
    24601 cgcactagag tcacaaatac tgaacagttg gtcttcattg ccgcattgaa tatcagctat
    24661 gagttagcgc aagaaaaagc aaagactcgt gactacgcgg caagtatgga acagcgtatt
    24721 cggatgctgc agcagaccat agaacaagcg ttacttgaac aaggtcgcat caccgaaaaa
    24781 actaaccaaa actttgaatg acacttttcg gtttactgtg gtagagtaac cgtgaagaca
    24841 aaatttctct gagatgttcg caagcgggcc agtcccctga gccgatattt cataccacaa
    24901 gaatgtggcg ctccgcggtt ggtgagcatg ctcggttcgt ccgagaagcc ttaaaactgc
    24961 gacgacacat tcaccttgaa ccaagggttc aagggttaca gcctgcggcg gcatctcgga
    25021 gattcccttc ttatctggca ccagccatga cgcaactacc agagctccca ctgacattat
    25081 cccgacaaga aatccgcaaa atgattcggc aacgtcgtcg tgcgttaacg ccggaacaac
    25141 agctggaaat gggtcaacaa gccgctaccc ggatgatgac ttatcccccg gtggtgatgg
    25201 cgcatacggt cgctgtattc ctctcttttg atggtgaact cgacacccag ccgctaatag
    25261 aacaactctg gcgcgccggt aagcgcgtat atcttccagt tttgcatccc tttagtgccg
    25321 gtaatttgct gttcctgaat taccatccgc aaagcgaact ggtgatgaac aggttgaaga
    25381 tccatgagcc aaaactggat gtgcgtgacg tgctacccct ttcccgatta gacgtgctga
    25441 tcacaccgct ggtcgccttt gatgagtacg gtcagcgcct gggaatgggc ggtggttttt
    25501 atgatcggac cttacaaaac tggcagcact ataaaacgca accggtgggt tatgcgcatg
    25561 attgtcagtt ggtggaaaaa ctccccgttg aagagtggga tatccctctt cctgcggtgg
    25621 ttacaccgtc gaaagtctgg gagtggtaag ggcgatacac ctgcatcgcc ctgattgaca
    25681 tcgttgattc tttaacctaa tttagtgagt aagggtaagg gaggattgct cctcccctgt
    25741 gactgttaat aagctgggaa acttatgagt aacagtacaa tcagtatgat ggcaagtcgc
    25801 atcataaccc ttctccttca agccctcgct tcggtgaggg ctttaccgtt acagccccat
    25861 gctgcccagc tatcgtaagc tctctttaaa taaccacaac gcattgatct acctttaaat
    25921 cttttcttcg agcgtgctcg aaaaacccca tgtaaaacag agataatttt tgggaaaaag
    25981 gatctattac ggcagtaaac gtgggcgatg aagtggctat caaaaagttc ggttcagggc
    26041 gatgcacccc gcatcgccct gattgacatc gttgattctt tgacctaatt tagtgagtaa
    26101 gggtaaggga ggatgactcc tcccttgaga ctgactgtta ataagctccg aaacttatca
    26161 gtaacagtac aatcagtatg atgacaagtc gcatcataac ccttctcctt taagccctcg
    26221 cttcggtgag ggctttaccg ttacagcccc atgctgtccc gccatcgtaa atccccatta
    26281 aataaacaca acgcattgat ctgactttga tttattttct ggagcagact cgcaaagtag
    26341 aatgcgcaac gcggcaacgg tgtggagaag ggataaaaaa acgggcaagt cagtgacctg
    26401 cccgttaatt ttcagagaag gggaattagt acagcagacg ggcgcgaatg gtacccggaa
    26461 tagctttcat tgcctgcagc gctttttcgg caacgtcttc gtcagcttca atatcaataa
    26521 ctacataacc catctgggcg gaagtttgta gatattgcgc ggcgatgttg acgccctgct
    26581 cggcgaagat tttgttcagc gcagttagca cgcccggacg gttttcgtgg atgtgcatca
    26641 gacgacgacc accatgcagt ggcagcgaga cttccgggaa gttcaccgca gagagcgttg
    26701 agccattgtc agaatacttg atcaatttac ccgcaacttc caagccgata ttctcctgcg
    26761 cttcctgagt cgaaccgcca atgtgtggcg tcagaaggac gttgtcgaat tcacacagtg
    26821 gagaggtaaa tggatcgcta ttggtcgccg gttccgtcgg gaatacgtcg attgccgccc
    26881 ccgccagatg tttgctcgcc agcgcatcac acagcgccgg aatatccacc acagtaccgc
    26941 gcgaggcatt aatcagcagc gagccgggct tcattagcga aatctctttc gcgcccatca
    27001 tatttttggt ggacggattc tctggtacat gcagactcac cacatcgctc atattcagca
    27061 ggtcagaaag atgctgtacc tgagtggcgt tgcccagcgg cagtttgttt tcaatatcat
    27121 aaaagtaaac atacattccc agcgattcag ccagaatgcc caattgcgta ccaatatgac
    27181 cgtagccgat gatacccagc tttttgccgc gcgcttcaaa agaacccgcc gccagtttgt
    27241 tccacacgcc acggtgcgct ttagcattgg cttccggcac gccgcgcaat agcagcagca
    27301 gttcgccaat caccagctcc gcaacagagc gcgtatttga gaacggtgcg ttaaataccg
    27361 ggatcccgcg ctttgccgcc gcatccagat caacctggtt tgttccgata cagaaacagc
    27421 caatagcgac cagtttttct gcggcgttga tcacgtcttc agtcagatgg gtacgggatc
    27481 gcaggccgat gaagtgggca tcgcggatgg attcttttaa ttgttcatca tccagcgcgc
    27541 ctttgtgaaa ttcgatgttg gtgtaaccag ctgcacgaag gctttccagc gccttttggt
    27601 gcacgccttc taccagcaga aacttaatct tgtctttctc cagcgatacc tttgccattt
    27661 acccaatcct gtcttttgaa atgttgtgtg cggatttgca tccgcctttc aacatatcaa
    27721 aaaataatat tgcggcaata tgaacgtttg cgtcgcgatg ttgaggaaat atcacgcaaa
    27781 ggtaattctt ggatgaatat gctggtttag aggatttatt tagagcaatc gacaattgcc
    27841 tggtaaaagc gtgacacatg tcaccaaatt taatgaagag aattttttta acgggggagg
    27901 ttcccccgtc agatcatttc acaatggttt tgacaccgtc aggtgtgcca atcagcgcaa
    27961 cgtccgcgcc acggttagca aacaagccaa cagtcaccac gccaggaatc gcatttatgg
    28021 cgttttccat cgctatcggg tcaaggattt ccatgccgtg gacgtcgagg atcacgttgc
    28081 cattatcggt caccacgccc tgacggtatt ccggacgacc gcccagtttc accagctggc
    28141 gcgccactgc actacgtgcc atcgggataa cttctactgg cagcgggaat ttacccagaa
    28201 tatcaacctg cttggaggcg tcggcaatac agataaattt ttccgcaacc gaagcaatga
    28261 ttttttcacg ggtcagcgcc gcgccgccgc ctttgatcat ttgcatgtgg ccgttgattt
    28321 catctgcgcc atcaacgtag atgccaaggc tgtcgacttc gttgagatca aaaacgtgaa
    28381 tgccgaggct tttcagtttt tcagtggaag catctgaact ggaaacggcc ccttcaatct
    28441 ggcctttcat cgtgccgagc gcgtcaataa agtgtgcggc ggtggaacct gtacctacac
    28501 caacaatggt accgggctga acatactgaa gtgccgccca tcctactgct tttttcaatt
    28561 catcctgcgt catgatcgtt tcgcctgtgg tatgaaattt cacacgcatt atatacaaaa
    28621 aaagcgattc agaccccgtt ggcaagccgg gtggttaact catccataaa atatcgcgca
    28681 atggcgggca tcccctttcg ccccgcaaat aaagcataca acggtctggg tatgccgctc
    28741 cacggtgcga acagaggcaa taacgccacg ccattcaaca taaccagggc gcgaagtcgc
    28801 taagcgaaat gcaatagcgc aaatccctga tcttttaagc tgcgcgttta accattgata
    28861 caaatgttac ccgccgacgg cttcggtata tgcaacctga cacaaaattg tgtcatagtg
    28921 caggaaaaag catttaccag gagcagacaa cagcaatgaa acgcccggac tacagaacat
    28981 tacaggcact ggatgcggtg atacgtgaac gaggatttga gcgcgcggca caaaagctgt
    29041 gcattacaca atcagccgtc tcacagcgca ttaagcaact ggaaaatatg ttcgggcagc
    29101 cgctgttggt gcgtaccgta ccgccgcgcc cgacggaaca agggcaaaaa ctgctggcac
    29161 tgctgcgcca ggtggagttg ctggaagaag agtggctggg cgatgaacaa accggttcga
    29221 ctccgctgct gctttcactg gcggtcaacg ccgacagtct ggcgacgtgg ttgcttccgg
    29281 cactggctcc tgtgttggct gattcgccta tccgcctcaa cttgcaggta gaagatgaaa
    29341 cccgtactca ggaacgtctg cgccgcggcg aagtggtcgg cgcggtgagt attcaacatc
    29401 aggcgctgcc gagttgtctt gtcgataaac tcggtgcgct cgactatctg ttcgtcagct
    29461 caaaaccgtt tgccgaaaaa tattttccta acggcgtaac gcgttcggca ttattgaaag
    29521 cgccagtggt cgcgtttgac catcttgacg atatgcacca ggcctttttg cagcaaaact
    29581 tcgatcttcc tcctggcagc gtaccctgcc atatcgttaa ttcttcagaa gcgttcgtac
    29641 aacttgctcg ccagggcacc acctgctgta tgatcccgca cctgcaaatc gagaaagagc
    29701 tggccagcgg tgaactgatt gacttaacgc ctgggctatt tcaacgacgg atgctctact
    29761 ggcaccgctt tgctcctgaa agccgcatga tgcgtaaagt cactgatgcg ttactcgatt
    29821 atggtcacaa agtccttcgt caggattaat ccatcaaata atgcctgata gcacatatca
    29881 ggcgttgtcc tcacttcttt ttgtattcct tgaatcacat cacaaaatag acaa
//
LOCUS       DADMGC010000038        29395 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.038, whole genome shotgun sequence.
ACCESSION   DADMGC010000038 DADMGC010000000
VERSION     DADMGC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.038"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..105)
                     /locus_tag="JNZ73_001669"
     CDS             complement(<1..105)
                     /locus_tag="JNZ73_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic ABC transporter ATP-binding protein"
                     /protein_id="HAY8731984.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQV"
     gene            complement(175..1164)
                     /locus_tag="JNZ73_001670"
     CDS             complement(175..1164)
                     /locus_tag="JNZ73_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8731985.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVETPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1961..2464
                     /locus_tag="JNZ73_001671"
     CDS             1961..2464
                     /locus_tag="JNZ73_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HAY8731986.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(2544..2795)
                     /locus_tag="JNZ73_001672"
     CDS             complement(2544..2795)
                     /locus_tag="JNZ73_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HAY8731987.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(2910..2996)
                     /gene="azuC"
                     /locus_tag="JNZ73_001673"
     CDS             complement(2910..2996)
                     /gene="azuC"
                     /locus_tag="JNZ73_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HAY8731988.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            3259..3582
                     /locus_tag="JNZ73_001674"
     CDS             3259..3582
                     /locus_tag="JNZ73_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731989.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLH
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            3753..4250
                     /gene="ftnA"
                     /locus_tag="JNZ73_001675"
     CDS             3753..4250
                     /gene="ftnA"
                     /locus_tag="JNZ73_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HAY8731990.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(4287..4526)
                     /locus_tag="JNZ73_001676"
     CDS             complement(4287..4526)
                     /locus_tag="JNZ73_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HAY8731991.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            4716..5927
                     /gene="tyrP"
                     /locus_tag="JNZ73_001677"
     CDS             4716..5927
                     /gene="tyrP"
                     /locus_tag="JNZ73_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HAY8731992.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSMPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVPSPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(5989..6654)
                     /gene="yecA"
                     /locus_tag="JNZ73_001678"
     CDS             complement(5989..6654)
                     /gene="yecA"
                     /locus_tag="JNZ73_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HAY8731993.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(6850..6936)
                     /locus_tag="JNZ73_001679"
     tRNA            complement(6850..6936)
                     /locus_tag="JNZ73_001679"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6900..6902),aa:Leu,seq:taa)
     gene            complement(6949..7022)
                     /locus_tag="JNZ73_001680"
     tRNA            complement(6949..7022)
                     /locus_tag="JNZ73_001680"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6988..6990),aa:Cys,seq:gca)
     gene            complement(7304..7852)
                     /gene="pgsA"
                     /locus_tag="JNZ73_001681"
     CDS             complement(7304..7852)
                     /gene="pgsA"
                     /locus_tag="JNZ73_001681"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAY8731994.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(7909..9741)
                     /gene="uvrC"
                     /locus_tag="JNZ73_001682"
     CDS             complement(7909..9741)
                     /gene="uvrC"
                     /locus_tag="JNZ73_001682"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAY8731995.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(9738..10394)
                     /gene="uvrY"
                     /locus_tag="JNZ73_001683"
     CDS             complement(9738..10394)
                     /gene="uvrY"
                     /locus_tag="JNZ73_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HAY8731996.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            10690..10866
                     /locus_tag="JNZ73_001684"
     CDS             10690..10866
                     /locus_tag="JNZ73_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8731997.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            10853..11077
                     /locus_tag="JNZ73_001685"
     CDS             10853..11077
                     /locus_tag="JNZ73_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HAY8731998.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(11110..11730)
                     /gene="sdiA"
                     /locus_tag="JNZ73_001686"
                     /pseudo
     CDS             complement(11110..11730)
                     /gene="sdiA"
                     /locus_tag="JNZ73_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(11960..12712)
                     /gene="yecC"
                     /locus_tag="JNZ73_001687"
     CDS             complement(11960..12712)
                     /gene="yecC"
                     /locus_tag="JNZ73_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HAY8731999.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(12709..13377)
                     /gene="tcyL"
                     /locus_tag="JNZ73_001688"
     CDS             complement(12709..13377)
                     /gene="tcyL"
                     /locus_tag="JNZ73_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HAY8732000.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(13392..14378)
                     /gene="dcyD"
                     /locus_tag="JNZ73_001689"
     CDS             complement(13392..14378)
                     /gene="dcyD"
                     /locus_tag="JNZ73_001689"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HAY8732001.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(14483..15283)
                     /gene="tcyJ"
                     /locus_tag="JNZ73_001690"
     CDS             complement(14483..15283)
                     /gene="tcyJ"
                     /locus_tag="JNZ73_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8732002.1"
                     /translation="MKLAHLGRQALMGVMGVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(15371..15922)
                     /gene="fliZ"
                     /locus_tag="JNZ73_001691"
     CDS             complement(15371..15922)
                     /gene="fliZ"
                     /locus_tag="JNZ73_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HAY8732003.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(15968..16687)
                     /locus_tag="JNZ73_001692"
     CDS             complement(15968..16687)
                     /locus_tag="JNZ73_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HAY8732004.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVCHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(16852..18504)
                     /locus_tag="JNZ73_001693"
     CDS             complement(16852..18504)
                     /locus_tag="JNZ73_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HAY8732005.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTVGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIILQAGNSVLAKANQVPQQVLSLLQG"
     gene            18770..20176
                     /gene="fliD"
                     /locus_tag="JNZ73_001694"
     CDS             18770..20176
                     /gene="fliD"
                     /locus_tag="JNZ73_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAY8732006.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            20201..20611
                     /gene="fliS"
                     /locus_tag="JNZ73_001695"
     CDS             20201..20611
                     /gene="fliS"
                     /locus_tag="JNZ73_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAY8732007.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            20611..20976
                     /gene="fliT"
                     /locus_tag="JNZ73_001696"
     CDS             20611..20976
                     /gene="fliT"
                     /locus_tag="JNZ73_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HAY8732008.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            21054..22541
                     /gene="amyA"
                     /locus_tag="JNZ73_001697"
     CDS             21054..22541
                     /gene="amyA"
                     /locus_tag="JNZ73_001697"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY8732009.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDCIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(22575..22988)
                     /locus_tag="JNZ73_001698"
     CDS             complement(22575..22988)
                     /locus_tag="JNZ73_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8732010.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            23175..24380
                     /gene="yedE"
                     /locus_tag="JNZ73_001699"
     CDS             23175..24380
                     /gene="yedE"
                     /locus_tag="JNZ73_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HAY8732011.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            24377..24610
                     /gene="yedF"
                     /locus_tag="JNZ73_001700"
     CDS             24377..24610
                     /gene="yedF"
                     /locus_tag="JNZ73_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAY8732012.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            24717..25387
                     /locus_tag="JNZ73_001701"
                     /pseudo
     CDS             24717..25387
                     /locus_tag="JNZ73_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(25427..26617)
                     /locus_tag="JNZ73_001702"
                     /pseudo
     CDS             complement(25427..26617)
                     /locus_tag="JNZ73_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            26685..27300
                     /gene="tnpA"
                     /locus_tag="JNZ73_001703"
                     /pseudo
     CDS             26685..27300
                     /gene="tnpA"
                     /locus_tag="JNZ73_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            27628..28710
                     /locus_tag="JNZ73_001704"
     CDS             27628..28710
                     /locus_tag="JNZ73_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAY8732013.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKVNL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(29199..>29395)
                     /locus_tag="JNZ73_001705"
     CDS             complement(29199..>29395)
                     /locus_tag="JNZ73_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY8732014.1"
                     /translation="EGISFQSILDDVRLNNALSAIQTTVKPISEIARENGYKCPSRFT
                     ERFHNRFKITPRELRKASRE"
BASE COUNT         7416 a         7403 c         6874 g         7702 t
ORIGIN      
        1 aacctgaccg gcatagcagt caaaactaat atccgtcagc gccttaacgc cgggaaacgt
       61 tttaccgatg ccgcgaaatg agagatacgg ggtagactgt tgcataacgt ctccgtgaat
      121 cactggtcgt tcacaccccc tctgcatcat gcagagggga atttttccgg caaattactt
      181 accgcctaaa ccttttttct ccagttcttc tttaaagttg tcacgcgtga tcagtaccac
      241 gtctgtaact tcggtaaatt ttggcgtttc aacgtctttt gctacccagt tgtaaagcat
      301 ttcgctggat ttatagccat gtacgtccgg gcttggcagc agggaaccgt agaagccggt
      361 tgcctgtgct ttagacagtt cgcttactgc atccacaccg ttaataccaa taccgatgat
      421 atcggctgct ttaaagccct gaccttcggt tgcacgtacg ccgccaagta cggtgctgtc
      481 gttcataccg acgatcagcc aatgtttaac ttccggatgt tgaaccagca ttgagttggc
      541 agcgtcaaat gcccccggga tgtcgttaga tttggtaggt acctgataaa tttgtttttc
      601 cgggaatcct gcagctttca gtgcgtccat agaacccgtt gtacggcggc gggcggtatc
      661 cagttcgttg gcggtaatcg ccatcactgc gctttctttg acatcccagc cacgtttctg
      721 catctcttta tacagttcct ggccctgacg ttcgccaatt ttagtcgccg ccatcatcac
      781 cagaggaacg gtatccattg gcttaccttt ggcgttaaca aactggtcat ccactgcaat
      841 gactttcata tcgtagccac gcgctttcgc gacgatggca gagccgagtt tcgggtccgg
      901 agtacaaata acgaaacctt ttgcgccact ggctgccagg ctgtcgatcg cgttcaatgt
      961 tttttcgcca tccggcacgg caatcttaat aacctcaaac cctaaatcct tcccggcttt
     1021 atcggcaaac ttccattcgg tctggaacca cggctcttcc ggttgcttca ccagaaaacc
     1081 gagcttcagg ttctccgcca tagcggattg tgacataacg gctgccagac caatggctgc
     1141 cagggcttta gtaaatttgt gcatggttct ctccagcttt agtgtcgttt tgtgtagggc
     1201 aaaaacgaat gacattcgtt aaattaatcg gaaaacaaag cattaccttt tatctaaaag
     1261 ataagtgacc gtgttgacat agttttagcg agaaattaat tctccatagg agagcaatat
     1321 cacatcgcag aattacagtg agaacgtgca taaatttagc gggaaaagac ataaggaaaa
     1381 gccaatttgt cagacaaatt gttgaatgca cagcagatta atccataaga ttagcctgga
     1441 aatccttgtt gtcttcggta cccatgcggg atgtcttctt tttaaccagt caataggccg
     1501 cattacctgg cgttgagttt ttgaaatggt gtaataacca caactcaaag atgtggaaaa
     1561 tgcgcgtcat tcatttcgcc attaattatc actgtgctca ttaattaaca gaacacgtat
     1621 aatgagagcc atctcgcaaa aatgaaaaaa cgttttataa aatcatcact tcatcatgaa
     1681 ttcaaattca tcgattaata tcaacaagat acaaaaagca ctatcattaa aattcattgc
     1741 atttacattg atttcaccaa tgaaatgtaa aaatatataa acttgatgat ttaagcattt
     1801 tcttataacc gttcagacgt tattcttatt tcagatcatc gtcagaattg actccacgat
     1861 cacatttcgg accggcagaa aggaattatt ctgcaaacag taattatggt gttttgattt
     1921 atcttgcacc tctccacttc tggatataag gatattaggt atggcaaccg ctggaatgct
     1981 tctcaaactc aactctcaaa tgaaccgcga gttttacgca tccaatctct accttcacct
     2041 gagtaactgg tgttctgaac agagtctgaa cggcaccgcc actttccttc gcgcccaggc
     2101 acagagtaat gtgacccaaa tgatgcgcat gtttaacttt atgaagagtg tcggcgctac
     2161 ccccatcgtt aaagccattg atgttccagg tgaaaaactg aactctctgg aagaactgtt
     2221 ccaaaaaacg atggaagaat acgagcaacg ttcaagcacg ctggcgcagt tagccgatga
     2281 agcgaaagaa ctgaatgatg attcaaccgt caatttcctg cgcgatctgg aaaaagaaca
     2341 gcagcatgat ggcctgttgc tgcaaaccat tcttgatgaa gtgcgcagtg cgaaacttgc
     2401 gggtatgtgc cctgtgcaga ccgaccaaca tgttctgaat gtcgtgtcac accagctgca
     2461 ttgatcatac atcggcgcta atacattgcg ccgatgaagt ttttgagaaa ccgctgcctc
     2521 atctgtttga agcagcggtt tttttaatgg gattcaccct gtggggtaaa cttgagttca
     2581 atcagcgcga tggctttttg gattgcccgc atggtgaccg ggtctgcggc agcgggatga
     2641 tgagtaaagt cgatattctt cagctgactg gacatttttt cacgaacttc aacgggcgcg
     2701 attacatcga gaacatccag aatttgtttg ataaccaact ggcaagctac cacatcagaa
     2761 accagttcct gatcggcatt cagcggctgg gacatcgtaa actcctgata gcattttgaa
     2821 agccgttata gtagcggctt cacatcttca gcgatagtca catccaccgt catcaggaca
     2881 caaaaaaacc tgccggagca ggttttttgt tatcggaaca tattgcctgg cggtacgtct
     2941 ttgaacgtct tgcaatagtt attgaacata cttttcagga ttttgcgcag tttcatcgcg
     3001 gcactccgac catttgttat acaggtgtta ttgtctttgc gcttataata tgacaaccat
     3061 cacaaaaatc aatctttatg tgatacaaat cacataaata cccctttaat gttataaaaa
     3121 tgataatcaa aaaacagccc ccctatttct gacacctaca gatggcaaga aatagcgcct
     3181 gccaggcatc ttttccggcc attgtcgcag cgctgtaacg cgtaaaatag tgctttctct
     3241 tactcttctg gctggaccat gagacttctg attctgactc tttcattaat aacgctggcg
     3301 gggtgtacgg ttactcgcca ggcccatgtg agcgaagttg acgcggcaac aggtattgta
     3361 cggttggttt atgaccaggc ttttttgcat catgcccata ccgatcgtta tgtcagtcgc
     3421 gggatcgccg atcgcgcatg ccagcaggaa ggctataccc acgcgatccc ctttggtcag
     3481 ccagtaggca actgtagcct ttttgcgggt tccttatgtt tgaatacgga attcactttg
     3541 tcatatcagt gccatcattc cgccttccct gtctttctct aaatatttta atcagcgagg
     3601 ggatcttcgc tgattaaaga aatagcggta atgcgtttta ttcccattcg tatttttaat
     3661 aattgaagtt tatattttac cttttgcaaa taataaaata acaaattata gtgacgccac
     3721 agcaacaaat ataacctttg tggagcacta tcatgctgaa accagaaatg attgaaaaac
     3781 ttaatgagca gatgaacctg gaactgtact cttcactgct ttatcagcaa atgagcgcct
     3841 ggtgcagcta tcataccttc gaaggtgctg ccgcgttcct gcgccgtcac gcccaggaag
     3901 agatgacgca tatgcagcgt ctgtttgatt acctgactga taccggcaat ttaccgcgta
     3961 ttaataccgt tgaatctccg tttgctgaat attcctcact tgatgaatta ttccaggaaa
     4021 cctataaaca cgaacaatta atcacccaga aaattaacga actggctcat gctgcaatga
     4081 ccaatcagga ctacccaaca tttaatttcc tgcagtggta tgtttctgag cagcatgaag
     4141 aagagaaact gttcaaatcg attattgata aattaagcct ggcaggcaaa agcggcgaag
     4201 gtctgtattt tatcgacaaa gaactctcta ccctcgacac acaaaactaa tgctctccgg
     4261 cggcaggttt ctgccgccgt ttcaaatcag tgacggcaaa tcttactctg atcggtcgaa
     4321 aaaccttctt ctgaaggtat aaatttgcct tttgctgcca gaaacgctac cagctctccc
     4381 gctgtcatcc cttctgccga gcacgtgtga aaacgtgcct gttcaccaaa acgcgcttta
     4441 atcgcagctt ccagactggc atgcgtatat tgctcgcctg attcaatcat catatttaac
     4501 acttcatgac cgtgaataga gtccatcgtt cctcctcaaa aaaagcctag cgtagcgatt
     4561 accgcttatg aagactttgc gccagcgcag gactgaatgc ttttattgta catttatatt
     4621 tacacaatat gtaacgtcga tttgacgaag cagccgttat gccttaacct gcgccgcaga
     4681 tatcactcat aaagatcgtc aggacagaag aaagcgtgaa aaacagaacc ctgggaagtg
     4741 tttttatcgt ggcgggaacc acaattggcg caggcatgct ggcaatgccg ctggctgcgg
     4801 ccggtgttgg ttttagcgtt acgttaatct tgttgattgg gctttgggcg ttgatgtgct
     4861 acacggcgct attactgctg gaggtgtacc agcatgttcc ggcagatacc ggtctgggca
     4921 cgctggcaaa acgctatctg ggacgctacg gtcaatggct gacgggcttc agtatgatgt
     4981 tcttaatgta tgctctgact gcagcataca tcagcggtgc cggtgaattg ttggcctcca
     5041 gcatcagcga ctggacaggt atttcaatgt cggcaaccgc tggcgtgctg ttgttcactt
     5101 ttgttgccgg tggcgtggtt tgtgttggaa catcgctggt cgatttattt aaccgttttc
     5161 tgttcagcgc caaaattatt tttctggtgg tgatgctggt attgctgcta ccgcatattc
     5221 acaaagtgaa tcttttaacc ctgccgttgc aacaggggct ggcactgtct gcaatcccgg
     5281 tgatttttac gtcgtttggt tttcacggta gcatgccgag tattgtcagc tatatggatg
     5341 gcaacattcg taagctacgc tgggtgttta taatcggtag tgccatcccc ctggtggcat
     5401 atattttctg gcaggtggcg acgcttggca gcattgattc aacaaccttt atgggattgc
     5461 tggctaatca tgctggatta aacgggctgt tacaggcgtt acgcgaaatg gtgccctctc
     5521 cgcatgttga gctggcggtg catttatttg ctgatttagc cctcgccacg tcatttctcg
     5581 gcgttgcgtt aggcttattt gattatctgg ctgatttgtt tcagcgttca aataccgttg
     5641 gtggacgatt gcaaactggt gcaattacct ttctgccgcc gttggcgttt gcactgtttt
     5701 atccacgagg atttgtgatg gcgctgggtt acgccggtgt ggcgctggcg gtactggcat
     5761 tgattatccc ttcactattg acctggcaaa gcagaaagca caatcctcag gcgggttacc
     5821 gggtcaaagg tggtcgtccg gcgctggtgg tggtgtttct ctgtggtatt gctgtgattg
     5881 gcgtgcaatt tttgattgcg gcagggttgt taccagaagt ggggtgatca gatagcctca
     5941 aattccttat tgggtgccag aattaacgct gacacccaat gtgacctctt aatgcaggca
     6001 gcactgctta aatttcttac cactgccgca cggacaagga tcgttacgcc ccggtttctc
     6061 ttctgctttg atcggttgct gaacagcttt ttcctgcgga tgcgccatcc agtatgcatg
     6121 taaatcaagc gccgccagtc gaatggcatc tacgctctct tcaaacgctt ctggcgacat
     6181 tttctctacc cgctcgaagt tttcctcagt accgtgcagc gcaatcgcct ccagcgctgg
     6241 ttttaaagaa tcgggcaacg ttgaccagtc agaaagtgcc acgccccgca tatagccaaa
     6301 gcaccactcc tcaacaatcg tcagctcgct gccatcaact tctcgcaagc cgaataacgg
     6361 ctcaaactgc tccgggaatt cgttcagacg ctctgcggta tcggccatat gttgaaaagc
     6421 caggttcata aagcgcgtca tctctttctc tgacgcccaa cgcggcacat agtcagcccc
     6481 accccacacg gcaaccagcc actgttctgg ttcaatctct tgcggagaac tcaacaccgc
     6541 cgtcaataaa ccgtccagct ccgccacatc aaggatggcg tggtcagtgt tgtatttggt
     6601 cagaatatcg tccagccatt ccaactcact ttcgtttaac ggtcccgttt tcatacgctt
     6661 ttccttgtgg atctcaactc gccagcacct atcttacatg ccggtccgta tcagagatac
     6721 tttttgagtg gctttgccgg tgattaaaaa ttaaggaggg tgtaacgaca agttgcaggc
     6781 acaaaaaaac cacccgaagg tggtttcacg acactgctta ttactttgat tttattctta
     6841 tctttcccat ggtacccgga gcgggacttg aacccgcaca gcgcgaacgc cgagggattt
     6901 taaatccctt gtgtctaccg attccaccat ccgggctcgg gaagaaagtg gaggcgcgtt
     6961 ccggagtcga accggactag acggatttgc aatccgctac ataaccgctt tgttaacgcg
     7021 ccaaattctt caggcctttc agccagacat ccgcttgacg ccgatgtctt ttaaactgga
     7081 gcgggaaacg agactcgaac tcgagacccc gaccttggca aggtcatgct ctaccaactg
     7141 agctattccc gtattcatca agcaatcagt taatcacttg attttattat cgtctggcaa
     7201 tcagtgccgc cgttcgatgc gttgcattct acttacctgg cgcgatgagt caacgatatt
     7261 tttcaccact tttgatcgtt tgctgaaaat tacgccgaaa cgatcactga tcaagcaaat
     7321 ctgcacgcgc agcgctcaaa tattgcaaca ttgaccacag agtcagtacc gcagccacaa
     7381 agaaaagtgc aataccggcg tactcaaccc aaatgttcgg acgccacagc agccaggcca
     7441 acgccaccat ctgggcagtg gttttcactt tcccaatcca ggagacggcc acgctactgc
     7501 gtttacccaa ctccgccatc cattcgcgta gcgcagaaat aataatttca cgggcgatca
     7561 tcgttgccgc cggtaatgtc acccaccagc tgtgataatg ctcggttacc agcaccatgg
     7621 cgatagccac gagaacttta tctgccacag ggtcaaggaa agcaccaaac cgggtactct
     7681 ggttccagcg gcgtgccaga aaaccatcga accagtcagt caccgccgcg acgcagaaaa
     7741 tgagcgcggc ggcaaacggc gaccaggtga caggcagata aaagaccaat acaaagaatg
     7801 ggataaggat gacacggaac agtgtaagca acgtagggat attaaattgc ataatgacgg
     7861 gtaactatct gttgtcagta agattacccc tatgttgcta cagagacatc aatgtttcaa
     7921 cgaccagaag atcttttctg ccagaccttg cgaaataccc ggcacttttg caatttcctc
     7981 gacgctggcg ttacgtaaac cttgcaaacc gcccatatat ttcaacaaca tttgccgacg
     8041 ttttggcccg acgccttcaa tggtttccag ggaactggta tttttgacct tcgcccgttt
     8101 tttacggtgc ccgccaatcg cgtgatcatg tgattcatcg cgaatatgct ggataacatg
     8161 cagcgcgggt gaatctggcg gcaaactaaa tccctcacct tccggctcaa agaacagcgt
     8221 ttccagtcca gccttacgat ctgctccttt ggcaacgcca agtagcagcg gatgattttt
     8281 atcccatgag acatccagtt cggcgaagac atttttcgcc tgcgcaagct ggcctttgcc
     8341 gccgtcgata aggatcacat ccgggatctt actgtcgtca atggctttac cataacgccg
     8401 acgcagcacc tgattcatcg ccgcataatc atcgcccggc gtgatgcctg taatgttata
     8461 gcgccgatac tccgcacgca gcgggccgtt agcatcaaac accacacagg aagcgacggt
     8521 ttgttcgccc atggtatggc tgatgtcaaa gcactccatc cgcttcactt ccggcaattt
     8581 caacacgctg gcaagcgcgg tcagtcgctg gtgaacggta gattgctgcg aaagtttgct
     8641 ggttaaggcc gtcgccgcat tggtgcgcgc gagtttcaga taacgcgccc tatcgccgcg
     8701 aggtttggtt tgaacattaa tcttgcgtcc cgccagttct gaaagggaat cggcgagcag
     8761 cgttttatcg ctaagattaa aatcgagcag gatctcaccc ggtaaggtgc gcatctggct
     8821 gccttgtaaa tagaactgac ctacgaaggt ttctaccacc tcgctcagtt ccgtaccgcc
     8881 aggcactttc gggaaatagc tgcggctgcc gagcactttg ccctgacgaa tgaacaatac
     8941 gtggacacaa gccatgcccg catcgaacgc cacaccaata acgtcaaggt cgtcgccggt
     9001 attggaaaca aactgttttt cggtgacgcg tcgcaccgcc tgaatttggt cgcgaataca
     9061 tgccgcttct tcaaactcca gattctggct ggcagtttcc atacggctaa tgagttgcgt
     9121 aagcacctga tcatctttgc cagacaaaaa caggcgcaca tactcgacct gctgagcgta
     9181 ttcttcttca ctcaccagtc cttcaacgca cggtcccaga cagcgcccta tctggtattg
     9241 cagacacgga cgcgagcgat tgcgataaac actattttcg cactggcgaa tggggaaaat
     9301 cttttgcagt agcgccagtg tttcacgtac ggcatagcca ttcgggaacg ggccgaaata
     9361 ttcacctttg gcatgctttg caccacgatg catcgccaga cgcgggtggg tatcgccact
     9421 caggaagata aaaggataag atttatcatc gcgtagcaaa acgttgtaac gcggctgata
     9481 gagtttgatg tagttgtgtt ccagcaacag cgcttcggtt tctgtatgcg taaccgttac
     9541 atcaatttgc tggatctggg cgaccagcgc ttcggttttg cgcgaagcga ggttgctgcg
     9601 gaaatagctg gaaagccgtt ttttcaggtc tttcgctttg ccgacataga taaccgtacc
     9661 accagcatcg tacatgcgat aaacgcctgg ctggctggtt acggttttta aaaacgcttt
     9721 tgcgtcaaac tgatcactca ctgacttgat aatgtctccg cattacacag accatggcga
     9781 attgccaggt gagtcagctc aacatcgcca tgaatgttta gtttactgaa catacgatag
     9841 cggtagctgt tcaccgtttt cggactgaga ttgagctgtt ctgagatctc attgaccttc
     9901 tggcccttag tgatcatcag cataatctgc aattcacgtt cagacaaact ggcaaatggg
     9961 ctttctgttt tttctggttc gatctggctt aacgccattt gttgagcgat atcagaagca
    10021 atgtaacgct gccctgaata gacagaacga atcgcactca cgacttcctg cggagccgcg
    10081 cctttgctga ggtagccagc agcacctgcc tgcatgactt tcgctggtaa agggttttct
    10141 gtatggaccg taagcatgat gattttaaca tcagctgtgg aacgcgcgat tttacgcgtc
    10201 gcctcaagac cgccaatgcc cggcatactc atgtccatta gcaccacgtc aacggcattt
    10261 gtccggcacc acttaacggc gtcttcaccg cacgatgcct ctccgacgac ttttataccc
    10321 tttatatctt ccagaatgcg tcgtatccct gcgcgcacca gttcgtggtc atcaacaagt
    10381 agaacgttga tcaaaggaat atctccagaa atagggataa cgctactgat agttagtcat
    10441 tcgtatatta gcggttttta ttgcaacttt gaaacgttaa aaatggtggg tattcgattt
    10501 ttctctcgtt tttgtcaatt cgattgtcca cagttatggt cacgcccgtc accaacggct
    10561 taccggatcg tttcacagta agccctacga tgaaatgtga caaaaattga ctttattcag
    10621 caaaaatgaa aatcagcccg ctgaatatgt aacattaatt aacccgaaca atacgggcaa
    10681 aaaacattca tgataaaaat atttattggt cattatatta acgtatttta tagcactgcc
    10741 gatatcacgc tcaaaaaaca accactgcta tttttagcaa agcttatggt atactccgcc
    10801 gccttaacat ttttcaccgc aaattttcat tgcaacatga cgaggaaaat aaatgagtac
    10861 gcctgatttt tctactgccg agaataatca agaactggca aatgaagtct cctgcctgaa
    10921 agcgatgctg acgctgatgc tgcaggcgat gggacaagct gacgcgggcc gcgtgatgtt
    10981 aaagatggaa aaacagcttg cgctgatcga agacgaaacc caggctgcag tattttccaa
    11041 aacggttaag caaattaaac aagcctaccg tcagtaatat aaaaccggct gagagcgtgc
    11101 ctttcagccg gttattgaca aaatcatcgc tatctctgct gatgtcttcc cttccgccgt
    11161 ccatttcaga atttcttttt cgcgcttgct gaaattcatc tctggcgtca tcactatttc
    11221 atcatttaaa cgcatcagag ccatcagact ttcgcgcacc agtaactgca tttttaattg
    11281 cagttcatca ctaagaatgg gtatttcgcg cgtgctgcaa cgggaaaagg acaaaaagcc
    11341 cagcgcccga tttggcagca ttaaatactg agtgacaccg cggcgtaaac catgtgcgtg
    11401 cgcggcttcc cataatggct gtgcttcgct gaataagtca tcattccaca ttaaatggtc
    11461 ctgactaaag ttttcagggt tcagcacagg atcaattgcg agaaaatttt ttgcctgata
    11521 ataactaacc cacgactcag ggtaattggt gtagaaagcc actttaggtc gcgtgaatag
    11581 taccgggtgg cgaacacata acgaatagta atcgtactcc agctgctgag cctgaagctc
    11641 tatttcatgg tagacctctt ctgcggcctc catcctctga aaacgcaaca gcatcgtgcg
    11701 acgccagctg aaaaaatccg tatcctgcat agtaaaccgc aacgcccctg agagtgagta
    11761 tcatttataa tgataattca aactatcaca taaataagat ttatatataa tttatattat
    11821 tcaggcaatg aattactttt gcaagccatc gcattctctt atgttattaa tgagttatgc
    11881 tgatttgtta agcagtttta tcacgcttga aatggcgtcc cgccccgaca ggtgaatcgt
    11941 cggggctgat tttttcttat tattgcagca gaagcttctc gaggaactgg cgggtgcgag
    12001 gctgctgggg atcggcaaat aacgcttttg ctgccccctg ctcgactatc cgcccctggt
    12061 ccataaagat cgcccggtcc gcaacatccc gggcaaagct catttcgtgt gtcacaatca
    12121 ccatcgtgcg cttttcctgc gccagctggc ggatggtgtt caggacttcg cccaccagct
    12181 ctggatccag cgccgacgtt ggctcgtcaa acaaaatcac ctcaggacgc attgccagtg
    12241 cacgcgcaat cgcaacacgc tgctgttgac cgccagacaa acgacgtgga tagctggttt
    12301 ctttaccggc cagcccaact ttcgccagca gctcgcgagc gcgcgccgtg gcctcttctt
    12361 tcggttcgcc tttgacgatc accggccctt caataatgtt ttccagcacc gtacgatgcg
    12421 gaaacaaatt aaagttctgg aagacaaacc cgacgtgctg acgcaactgg cgaatcagcg
    12481 atttttgctg acttaatgaa cgtgcagtat caatagtgat atcgccaacg gtgatcgttc
    12541 ccgcttccgg ttgttccagc agatttatgc tacgtagcaa tgtggttttg ccggaaccac
    12601 tcggaccgat aattgccacc acttcgccag gctttacctc gaggtcgata ccgtgcagca
    12661 ccgtctgacc gtggaatttt ttcaccaggt tcttaacttc aatggcactc attttggttc
    12721 tctctcctgg cgattaagtt gattctcaaa atggttctgc aacgtcgata acacagtcgc
    12781 catgatccag tagatgagcg aagccgccag gtacatggtg aacacctcca gcgtgcgcga
    12841 ggtaatcaac tgcgcctgac ggaacagctc cggcacctgg attgtcgcgg ccagcgacgt
    12901 atcttttacc agactgataa agctgttcga cagcggcggc aacgccaccc gcgccgcctg
    12961 tggcaaaatc gcgcgacgca tggtctgcca cggcgtcata ccaatactgg cagcggcttc
    13021 ccactgacct ttatcaatag aagaaatggc ggcgcgcagc gtttcggcgg cataggcggc
    13081 agtgttcagc gacagaccaa tcattgctga cggaatggga tctaattcga taccaaactg
    13141 cggcaggccg taatagatca taaacagttg tgcgatgagc ggcgtaccac gaaaaataga
    13201 gatataaaaa cgcgccagcc agcgcacggg ccagattggc gacaggcgca tcagcgcgag
    13261 aataaacccg agtagtaagc caaaaaacat gccgccgata ctgagttgca gggtataccc
    13321 agcccctttc aacaggaacg gcaaggaatc aataaccagt tgtatacttt cttgcatgag
    13381 cgttcccgga gttaaacgtg gggatgatag gcgaacagcg caggcgcgcc gccggtatga
    13441 ataaacagaa tcggcccttc atctttgaag cgtttctgac tgataccgtc aatcagcccc
    13501 gccatcgctt ttccggtata cacaggatca agcagaatac cttccagacg cgccagcaat
    13561 ttcactgctt ccatgccttc gtcgttcggc acgccgtagc caggtgcaaa atagtcatcc
    13621 cagagtaaaa tttccgctga tgcggtcagc tccagttctt tcgcaatcgc ctgttgtagg
    13681 ttaaccactt tcggcaattg atcggcaacg gaacgcgaca cggtcacgcc aatcagttcg
    13741 ctttcaggca tcaggtgttc cagcccaaca gccagtccgg cgtgagttcc ggcactgccc
    13801 gatgcgacta ccaccgacga aatattaacc gccccttcac actgttgcgc gatttccagc
    13861 gcactctcca cataacctag cgcgccaaga gcattagaac cgccaaccgg aatgacatac
    13921 gggcgaaagc cttgtgcttc gactcgcgtc gccagctctt ccagttgggc attgggatcg
    13981 gtcagtgcgt cgcacatttc aatctgggta ttgaacagat ccagcaacaa acgattgccg
    14041 ttggttaaat agttttctgc ggttgtgcca ataggatttt ccagcagcgc cacgcagtgc
    14101 agaccgagtt tcgccgcgac tgcggcagtc tggcgcacat ggttagactg gatcgcgccg
    14161 gcagtaatca gcgtatcggc accttcgcgc agggcatctg ccgcgagaaa ttccagctta
    14221 cgtaatttat tgccgcccat tgccatgggg gtgacctcat cccgtttgat gaaaatttcc
    14281 cgtccaagat aatcagaaaa gcgcggcaga tattcgagcg gcgttggcgc gccgataaac
    14341 tccaaccgtg gaaaacgggt taaattatgc agtggcataa cagcctccga tgcgtgttgt
    14401 tgtgattttc ttattatgca cgctgaaaac gcgtaaataa aaaaggcgct agtgaaagcg
    14461 cccttttttg tcattatgct gattatttgg tcacatcagc accaaaccat ttttcggaaa
    14521 gagcttgcag agtgccgtct ttttgcattt ccgcaattgc atcattcacc gctttcagca
    14581 ggtcttcatt tcctttacgc agcgccacgc cagactcctg acgggagaat gcttcaccgg
    14641 ttactgccag cgtatcgttg gttttcttca ccagatccag cgccgccaga cgatcaacga
    14701 ggatcgcatc gatacgccct acgcgcagat cctgatattt ggtcgggtca tcatcatagg
    14761 tacgcacatc gacaccctga acattctgcc gcagccactc ttcatagttg gtgcccagac
    14821 cgacacccac ttttttgcct ttcagatcag cagccgtttt aatggtgcct tcgttacctt
    14881 ttttcaccag cgcctgaata ccggaaatgg tgtaaggggt agagaaatcg tattttttct
    14941 tgcgctcgtc agaaatggtg acctgattaa tcaccacatc aatgcgttta gagtccagcg
    15001 acgccagcat accgtcccat ttggtcggtt ttagtgacgc ctcaacgcca agatgttttg
    15061 ccagctgttg agcaaattcc acttcaaaac cggttaattt gccgtcatcc ccctgaaaac
    15121 tgaacggcgg ataagtgcct tccagcccta ccagcagcgt gccgcgctct ttgactttat
    15181 taagcagacc ttcatctgca aaacttttaa cgctcatgcc cgcaaccagc gccacgccca
    15241 tcacacccat caatgcctga cgtcccagat gtgctaattt catattcacc ccgaatgttg
    15301 ttatgtctgt ttgcagtgta gagccattgt ttgtaaacac aaaaacaact ccgctacatc
    15361 ttattcttat ttaatatata tcagaagcag gcaggctgga ggatttctgc acaagtccgg
    15421 tacaggtttg gcgctgatag tgttgatact tccgtaatgc gatgcggtaa ttgttggtgc
    15481 tggtggtggg cagccacggg gcgaggattt catcaaggaa accgtcctgc agctgatcga
    15541 gggaaatatt ttgctcatgt agatgattac ccaaacggcg cagacgaacg acatattcac
    15601 gcaccgtacc tgggctcatt tcagtttgct caaaaagaaa tttcttaaag ccgataatat
    15661 cgaaaaaatc actctgcgtt ttgcaatgta aatcaccgca aaatcggcac aatgccgccc
    15721 atgattgttg ttccgtttgc cagccttttt catcaagcag cgtgtccagg cgggaaatct
    15781 cgattttatt cactattttg ccgtcgcgaa ctaaggtaat gcgatcgagt aatttacggc
    15841 aatgcgcgca atgggtctgg ctgtgtttaa agtctttaag atagcggctt aatggccgtc
    15901 ttttcaggtg ctgcaccatc attaagaact cctggtagtc aaagttaaag tgcggcattt
    15961 actgacgtta taacttaccc agtttagtgc gtaaccgttt aatggcctgg ctgtgtaact
    16021 gactgacccg cgattccccg acctccagca ccgcgccaat ctctttgaga ttcagctctt
    16081 cctggtaata gagggttaat accagttttt cgcgctccgg caacgtttcg atggcttcca
    16141 tcacccgctg gcgcagatta ctgtccagca gttgttgtag cgggttttct cgctgatgat
    16201 catcagtaac cagttcgatg ctatcgccgt gctcttcacg ccactcatcg taggagaaga
    16261 gctggctgtt attggtgtcg agcagcattt gacgataatc ggcaatatcg atccctaaac
    16321 gttccgctac ctcagtttcc gtggcgttgc ggccaagttc ctgctccagt tgccctattg
    16381 cctgtgccac ttcacgcgcg ttgcgtcgca cgctgcgcgg cacccagtca cggctgcgaa
    16441 gttcatccag catagcgcca cggatacgct gcactgcgta agttgtaaat gccgttcctt
    16501 gtagggcgtc atagcgttcg acagcattaa gtaacccaat gccgcccgcc tgtagcagat
    16561 cgtcaagttc cacgctcgcg ggcagtcgaa cctgcaggcg caatgcttcg tgacacacca
    16621 gcgggacata acgctgccac agcgagtgtt tatccattac accttcagcg gtatagagtg
    16681 aattcacgat aaacagccct gcgttatatg agttatcggc atgattatcc gtttctgcag
    16741 ggtttttaat cggacgatta gtgggtgaaa tgaggggtta tttgggggtt acgcaaaaag
    16801 ccgaatgtaa aaaaccccgt cggtggcggg gtttgagagg cattcagacg attaaccctg
    16861 cagcagagac agaacctgct gcggtacctg gttggctttt gccagcacgg agttaccggc
    16921 ctgcaggata atctgcgctt tcgacatgtt ggacacttcg gtcgcatagt cggcgtcctg
    16981 aatacgggac tgcgcttcag acaggttggt agtggtgttg ttcaggttgg tgactgcgga
    17041 atccaaacgg ttttgcaccg cacccaggga ggagcggaat ttgtcgatgg agctgatggc
    17101 ttcatccaga gctttcaggg gatcggctgt cgtagctgct tttgtttcag catctgtcgt
    17161 caatttacca tcagcatctt tataaactgc cttaccgctg ccgttagtca ctgaaccatc
    17221 atcctgtagg taaaaatcta cagtatcaga tgtggtcaac gcctcgttgt tatcaacata
    17281 aaccgaatca acaccttttg ttacagcgcc agttgtgccg ttgacgctat aacctacagt
    17341 agtgaaatca acattgtttc ccgaggttga tacagcttta tcgaaagctg cctgatcaac
    17401 ttttgcacct gttactgaat aagtaatagt gtccggcgta gtactattac cggtaaagga
    17461 gatagaggta ccgccaatgt caatggttgt cgctttggta ttattggctg caagtgtgga
    17521 cagactagcc tcagtcaaag aagcaccaga gccgtttttc gttaagcgcc cagttgtatc
    17581 aatgtagagt ttatctccat tgcttgccgt aattttacca tcactgctga tctgaacatc
    17641 ctgtgcagaa ccatcgatag taatagtgcc cttagcggta tcaccaactc ccggggtcaa
    17701 atatttctga acatcggcag ctgaagccgt tgtggtatca aaagagtatg acttacttgc
    17761 actgtcatac ttgtaattcg ttgcactagc cgccgttccg aagccgttat taatggttgc
    17821 ctgaacagta tcaccatcac taactccagc cagcaaatca gacgctttag cagcatcgta
    17881 acccgcactc acagtatatt tgttgccgac cactgttgca ttagcagcta ccaagtcagc
    17941 tttagatgca gcagtattag ccacagcccc gccaccattc acattaaacc cattcagccc
    18001 cagcgtatca gagtcaattt tcttcagatc aatagtgata gtctggccgt cattcgcacc
    18061 aacctgaatt ttcatcgaac cgtcttttgc cagtacgttc acgccgttga actgggtctg
    18121 accggatacg cggtcaattt cgtcgagacg ggatttgatt tcgtcctgaa tggagtccag
    18181 atccgaatca gagttagtcc cggtagaagc ctgaaccgtc agctcacgga tacgctgtaa
    18241 gttgttgttg atttcggaca gcgcgccttc agtggtctgt gcaacagaaa taccgtcgtt
    18301 ggcgttacgt gcagcctgag tcaggccttt aatgttagaa gtaaaacggt tagcaatcgc
    18361 ctgacccgcg gcgtcatcct tcgcgctgtt aatacgcaag ccagaagaca gacgctcgat
    18421 agaactcgac agcgcagact ggttcttgtt gatattattt tgagtgatca gcgagaggct
    18481 gttggtatta atgacttgtg ccatgattcg ttatcctata ttgcaagtcg ttgattacgt
    18541 tttgggtttc cacccgtcgg ctcaatcgcc gtcaaccctg ttatcgtctg tcgtaaaaca
    18601 acctttagaa tttttttcac aaacagccat tttttgttag tcgacgaaat actcttttct
    18661 ctgcccctta ttcccgctat taaaaaaaac aattaaacgt aaactttgcg caattcaggc
    18721 cgataacccc ggtattcgtt ttacgtgtcg aaagataaaa ggaaatcgca tggcaagtat
    18781 ttcatcgctg ggagtcgggt caggtctgga tttaagttca atccttgata gcctcaccgc
    18841 cgcgcaaaaa gcgacgctaa cccctatttc aaatcagcaa tcgtcgttta ccgctaaact
    18901 tagcgcctac ggtacgctga aaagcgcgct gacgactttc cagaccgcca atactgcatt
    18961 gtctaaagcc gatctttttt ccgccaccag caccaccagc agcaccaccg cgttcagtgc
    19021 caccaccgcg ggtaacgcca tcgccgggaa atacaccatc agcgtcaccc atctggcgca
    19081 ggcgcaaacc ctgaccacgc gcaccaccag agatgatacg aaaacggcga tcgccaccag
    19141 cgacagcaaa ctcaccattc aacaaggcgg cgacaaagat ccgattacca ttgatatcag
    19201 cgcggctaac tcatcgttaa gcgggatccg tgatgccatc aacaacgcaa aagcgggcgt
    19261 aagtgcgagc atcattaacg tgggtaacgg tgaatatcgt ctgtcagtca catcaaatga
    19321 caccggcctt gataatgcga tgacactctc ggtcagcggt gatgatgcgc tacaaagttt
    19381 tatgggctat gacgccagtg ccagcagcaa cggtatggag gtctcggtcg ccgcccagaa
    19441 tgcgcagctg acggtcaaca acgtcgccat cgagaacagc agcaacacta tcagcgacgc
    19501 gctggaaaac atcaccctga acctgaacga tgtcaccacg ggcaaccaga cgctaaccat
    19561 cactcaggac acctccaaag cgcaaacggc gattaaagac tgggtgaatg cctataactc
    19621 gctaatagat accttcagca gcctgaccaa atacaccgcc gtagatgcgg gagctgatag
    19681 ccagagttct agcaatggcg cactgctcgg cgactccacg ctgcggacga ttcagacgca
    19741 gttgaagtcg atgctgagta ataccgtcag ttcttccagc tataaaacgc tggcgcagat
    19801 tggtatcacg accgatccca gcgatggcaa actggaactg gatgccgaca aactcaccgc
    19861 tgcactgaaa aaagatgcca gcggcgtagg tgcattgatt gttggcgatg gtaaaaaaac
    19921 cggcatcaca accaccatcg gcagcaacct gaccagttgg ctttcgacaa cgggcattat
    19981 taaagccgct accgatggcg ttagtaaaac cctgaataag ttaactaaag actacaacgc
    20041 cgccagcgat cgcattgatg cgcaggtcgc gcgctacaaa gaacaattta cccaactgga
    20101 cgttttaatg acctcgttaa acagcaccag cagctactta acgcagcagt tcgaaaacaa
    20161 cagtaattcc aagtaatcat tattcatcgg gagacaggtc atgtacgcgg caaaaggcac
    20221 ccaggcctat gcgcaaattg gcgtcgaaag caccgtaatg agcgccagcc aacagcagtt
    20281 ggtcaccatg ctatttgatg gagtgctgag cgcactggtt agagcgcgac tgtttatgca
    20341 ggacaacaat cagcaaggca aaggcgtctc tttgtcaaaa gcgatcaaca tcattgagaa
    20401 cggactgcgg gtgagtcttg atgaagagag caaagacgaa ttaacccaaa acttaattgc
    20461 tctttatagc tatatggtca ggcgtttgct gcaagccaat ttacgcaacg atgtctccgc
    20521 agtcgaagag gtggaagcat taatgcgtaa tattgccgat gcctggaaag agtctttact
    20581 ctccccttct tcgattcagg acccagtctg atgaacaatg caccgcattt gtatttcgcc
    20641 tggcaacaac tcgtcgaaaa aagccagctc atgttacgcc tggcaacgga agaacaatgg
    20701 gacgaactca tcgctagcga aatggcgtat gtgaatgcgg tgcaggagat cgcacacctg
    20761 actgaagagg ttgccccgtc cactacgatg caggagcagc tccgcccgat gctgcacctg
    20821 attctcgaca acgaaagcaa ggtaaagcag ttattacaga ttcggatgga tgaactggcg
    20881 aaactggtcg gtcagtcatc ggtgcaaaaa tcggtgttaa gtgcctatgg cgatcagggc
    20941 ggctttgtgc tggctccgca ggataacctc ttttgaatct taatgagccg atggctcgcg
    21001 aataatccga ttacggctac gcttctaata ttccccttga atggagtcga agaatgcgta
    21061 atcccacgct gttacaatgt tttcactggt attacccgga aggcggtaag ctctggcctg
    21121 aactggccga gcgcgcagac ggttttaatg atattggtat caatatggtc tggttgccgc
    21181 ccgcctataa aggcgcatcg ggcggctatt cggtcggcta cgactcctat gatttatttg
    21241 atctaggtga gtttgatcag aaaggcagca tccctactaa atatggtgat aaagcacaac
    21301 tgctggccgc cattgacgcc ctgaaacgta atgacattgc ggtgctgctg gacgtggtag
    21361 tcaaccacaa aatgggcgcg gatgaaaaag aagctattcg cgtgcagcgt gtaaatgctg
    21421 atgaccgtac gcaaattgac gaagaaatca ttgagtgtga aggctggacg cgttacacct
    21481 tccccgctcg tgccgggcaa tactcgcagt ttatctggga tttcaaatgt tttagcggta
    21541 tcgaccatat cgaacaccct gatgaagatt gcatttttaa aattgttaac gactataccg
    21601 gagaaggctg gaacgatcag gttgatgatg aactcggtaa tttcgattat ctgatgggtg
    21661 agaatatcga ttttcgcaat catgccgtga cggaagagat taaatactgg gcgcgctggg
    21721 tgatggaaca aacgcaatgc gacggctttc gtcttgatgc ggtcaaacat attccggcat
    21781 ggttttataa agagtggatc gaacacgtac aggaagttgc gccaaagccg ctgtttattg
    21841 tggctgaata ctggtcgcat gaagtcgata aactgcaaac gtatattgat caggtggaag
    21901 gcaaaaccat gctgtttgat gcgccgctgc agatgaaatt ccatgaagca tcgcgcatgg
    21961 gtcgcgacta cgacatgacg cagatcttca ctgggacgct ggtggaagcc gatccttttc
    22021 acgccgtaac cctcgttgcc aaccacgaca cccaaccgtt gcaagccctc gaagcaccgg
    22081 tcgaaccgtg gtttaaaccg ctggcgtatg ccttaatttt gttgcgggaa aatggcgttc
    22141 cttcggtgtt ctatccggac ctctacggtg cacattacga agatgtcggt ggtgacgggc
    22201 aaacctatcc gatagatatg ccaataatcg aacagcttga tgagttaatt ctcgcccgtc
    22261 agcgtttcgc ccacggtgta cagacattat ttttcgacca tccgaactgc attgccttta
    22321 gccgcagtgg caccgacgaa tatcccggct gcgtggtggt catgtcgaac ggagatgatg
    22381 gcgaaaaaac cattcatctg ggagagaatt acggcaataa aacctggcgt gattttctgg
    22441 ggaaccggca agagagcgta gtgacagacg aaaacggcga agcgaccttc ttttgcaacg
    22501 gcggcagcgt cagcgtgtgg gttatcgaag aggtgattta aatccatccc cggcggcaag
    22561 ccggggagat ttcattacgg cagtggcgtc ggtaacggcg ctttatccag cgcggcggca
    22621 cactctgccg tcgggcgatc cacacgctcc atcgtcatac catcgtactc aatggtgtta
    22681 ccgtcacgct caacctcgta cagctcacgt tttacggtaa cgttagtgag atcatcagac
    22741 atcaatgtca acttacccgg cacggcaatc ccccgctgcc actgacggca atccaaggta
    22801 tcgccctctt tagtgacaat caggctgcct atcgcttccg gactgaccag cgcacgttgc
    22861 ggccctttgg tctgccagta gcccgccagc cagtccggcg cgggggtttt cacgacattg
    22921 ttatagtttt cgacttcggc acagcccgcc agcaacatga gcgcgcctgc aattgcgagt
    22981 tttttcatca ttcgtcctgc atgagaagaa aagaggattg tggcattaaa gctctgatgt
    23041 cgccagcctt tccaggttca gagagaaatt tgatctaact attatttttg tgaattatta
    23101 ccccgaaaat aaatttgcat ggtgtatgat tcgcggctct tgtttcaaac cttctgagtt
    23161 cagaggctac attcatgtca tggcagcaat tcaaacacgc ctggttgatt aaattctggg
    23221 cacccatccc tgcggtcatc gcggcgggta ttctctccac ttactatttt ggcattactg
    23281 gcaccttttg ggctgtcacg ggtgaattta cccgttgggg cggtcagctc ctgcagctgt
    23341 ttggcgtcca tgctgaagag tggggttatt ttaaaattat ccatctggaa ggatcgccat
    23401 taacccgcat cgacggcatg atgatcctcg gtatgtttgg cggctgcttt gctgctgcgc
    23461 tgtgggccaa caatgtcaaa ctacgcatgc cacgcagccg tatccgcatt atgcaggcca
    23521 tcattggcgg cattatcgcc ggttttggcg cgcgtctggc aatgggctgt aacctggcgg
    23581 cgttctttac cggcattcct cagttctcgc tgcatgcctg gttctttgcc atcgccactg
    23641 ccattggttc atggtttggc gcgcgcttta ccctgctgcc catcttccgt attcccgtga
    23701 aaatgcagaa agtttctgcc gcctcaccgc tgacgcaaaa accggatcag gcgcggcgtc
    23761 gttttcgtct cgggatgctg gtctttttcg gcatgctggg ctgggcgctg ctcacggcga
    23821 tgaaccaacc aaaactgggg ctggcaatgc tgtttggcgt cggctttggt ttactgattg
    23881 aacgtgcgca aatctgcttt acttcggcgt tccgcgatat gtggatcacc ggacgtaccc
    23941 atatggcgaa agcaatcatt atcggtatgg cggtgagtgc catcgggatc ttcagttacg
    24001 tacagttagg cgttgaacct aaaatcatgt gggcgggacc aaatgcggta attggtggtt
    24061 tactttttgg ttttggcatc gtgctggcag gcggctgcga aaccggctgg atgtaccgcg
    24121 cggtagaagg ccaggtgcac tactggtggg tcggtctggg caacgtgatc ggctcaacga
    24181 ttctggcgta ttactgggat gatttcgctc cggcgctggc caccgactgg gacaaaatca
    24241 acctgctgaa aacttttggt ccgatgggag gcctgctggt gacatatttg ctgctgtttg
    24301 ctgcgctgat gttgattatc ggctgggaaa aacgcttctt ccgccgtgcg gcaccgcaga
    24361 ctgctaagga gatcgcatga aaaatatcgt tcctgattac cgccttgata tggtgggtga
    24421 accctgccct tatccggcag tcgcaaccct tgaggcgatg ccgcaattaa aaaaaggtga
    24481 gatcctagaa gtggtgagcg actgtccgca gtcgatcaat aatattccac tggatgcgcg
    24541 taatcacggc tacactgtgc tggatattca gcaagacggg ccgaccattc gttatttaat
    24601 tcagaagtaa tccctcattc ccgctgtggc ccacagcggg aacatcgtta cctctgacac
    24661 atagatttta cccgccccct ctcctattct tttcagtatg atctcaggag gttgctatgt
    24721 gtggacgctg tgcccaatcc caaacgcgtg aggattacct tgcgcttctc gcggaagata
    24781 ttgaacgcga tattccctac gatcccgaac ccattggcag atacaatgtc gcgccgggaa
    24841 ccaaagttct tctgctcagt gaacgtgatg aacaccttca tctggatccg gttttctggg
    24901 gatatgctcc cggatggtgg gataaaccgc cgctgattaa cgcccgcgta gaaactgcct
    24961 caaccagtcg catgtttaaa ccgctctggc aacatggtcg ggcaatctgt tttgccgatg
    25021 gctggtttga gtggaaaaaa gaaggcgaca aaaaaagcct tattttatct atcgcgctga
    25081 cggacaacct gtttttatgg ccgcgatagg tagcataccg tttgagcgtg gtgacgaagc
    25141 cgaaggattt ttgatcgtca ctgcggcggc agatcaaggt ctggtagata ttcatgaccg
    25201 ccgcccgctg gttctgtcgc cagaagctgc acgggaatgg atgcggcaag acattggtgg
    25261 taaagaagcc tcagaaatcg ctaccagaag ctgtgtgcca gcaaaccagt tcacctggca
    25321 ccctgtgtcg cgcacggtgg gtaatgtcaa aaaccagggc gcggagttaa ttcaacctgt
    25381 ttgttgacat cctccacgcc ctgaatgacg aggccccctg ctacgttcag gctgtcgcct
    25441 gaatcatttc ggtgggttcc tgcttcaacg agcggcctga ctgcaccatc tctccacagg
    25501 caagaacggc gtgccccgcc gctaaaatgt tacgagcgcc atttacatcg gcgttcgctg
    25561 tatatccaca tacctggcat ctgaatttac tttgtgacag gcgattttct ttcgctgtat
    25621 gaccacagca cgcgcaacgc tggcttgtgt acgctggccc gccacgccag agctgcttgt
    25681 actcaagctg gcggcgcatt tcataccagc cctgatccag tatcgaacgg tttaaacctg
    25741 attttgcccg gacattgcgc cccggctggc tgaccgtacc cgctgctgac tttgacatgt
    25801 tgctgacctt caaatcctca atgactatca ttacgtggtt tttgctgacg gtcgttgtga
    25861 ctttgtgaag gtagtccctg cggatatttg cgatacagga atgcagtcgc tgtattttgc
    25921 gtttctgctt ctgccagttg ttgctgaatt tgaccttgcg gcttaactgt cgctgaagtc
    25981 tcgccagcgt cttctggttt ttctgaaaac tgtttacagg ctcaaagact gtgccatctg
    26041 acagcgaggc aagtttagcc acgccagcat ccagcccgac cattgatgct gaagggtgaa
    26101 ccggagttga tacttcactt tctgcctgaa tactgatgta ccacttaccg caggactggc
    26161 tgacagtgac atttttcaca acacccgtga cctgacggct gttccggtag cgcatccagc
    26221 caagttttgg cagaaaaata cggctgtttt cctgatcgag cttaacgccc tgcggatagc
    26281 ggaatgcatc attctgtcac cgctttttga atcaggggaa cgccgaccgc ttctggaaaa
    26341 agtttttgca ggcctgctca gggtctttca gtgactgttg caatggctgt gacggagcat
    26401 ctttaatcca ttgcgtttca gtggcatttt tccactcaac caatcaggaa gccattttcg
    26461 cgtaagggat gtctttgtta ccggccttat gattctcatt ctgacgtgcc agagtacgac
    26521 tgaaaacgaa acgacatgcg cctgcgaagc gcctcatttc acgcgcctgt tgaccaccgg
    26581 gtcttaaccg gaatttgaat acctgtagtc gtttcacagg gcaaattata ctttggtctg
    26641 tgaaaaaaac tgatatccga cgcggaaggc actgtgtttt cctgatgcat gctcaccggg
    26701 tctttgttac caagtaccga cgccagattt ttgaccatga cgcgacagaa aaactacgta
    26761 cttacttttc aaatgtatgt gctgattttg aagctgaact ggttgaaatg gatggcgaac
    26821 cagagcacgt ccatctgtta attaactatc cccccaaact ggcgatatcc agtctggtaa
    26881 acaaacagcc tcaaaggcgt atcgggtagg ttactgcgac gggatcggcc agatattgct
    26941 gccaggtatt accgcaaaga tgttttgtgg cgtcccggct attttgccag tagctgcgga
    27001 ggtgcgccaa tatccgtcat ccgccaatac attgaacaac agcaaacacc tggtcaggtg
    27061 aaaaaacgag ccttatatcc ccgccctgaa ggacgggctt ttacggtgca ctggataagc
    27121 ctggagatta tctacacctg catggacatc attgtactat gccccgtaca tgccccatca
    27181 catcctcaac atcaaagtat tagagccttg caaaccatta tgcgtctcag tttgtctcat
    27241 ctatcttacg caaaaaaaat agaactaata tcaagcggcg ctatattgcg tattaaataa
    27301 aaccacctca attacattaa taaatctaaa taaatatcat acaaataatt tccatggtta
    27361 tttttaggct atttaaatta aaaaaataaa ttatgagtca atcataaata acaaccttta
    27421 atataatttg actaccaaat ttatattcgt tcacaaaata gtcatgcatt gtgtgcaata
    27481 gaaacagttc agataaaaat agggatctac ttcacaattt aaatgagaaa ctaaaactta
    27541 catcttgaaa taatctcatt gattggatga atatttgtag tgcagtgaca tcatttttaa
    27601 ataatagttc aaataaaagg gctcacgatg aaaaaattaa cagtggcaat ttctgctgta
    27661 gctgcatcag tactgatggc gatgtctgct caggcagcag agatttacaa taaagacagt
    27721 aacaagctgg atctgtacgg aaaagttaat gctaagcact acttctcttc taacgatgca
    27781 gatgatggcg acacaactta tgttcgtctg ggcttcaaag gcgaaactca aatcaacgat
    27841 caactgactg gtttcggtca gtgggaatat gaattcaaag gcaaccgtgc tgaatctcaa
    27901 ggttcttcca aagataaaac ccgtcttgcc ttcgctggcc tgaaattcgg tgactacggc
    27961 tccatcgatt atggccgtaa ctacggtgta gcatacgata tcggtgcatg gaccgacgtt
    28021 ctgccagaat tcggtggcga tacctggacc caaacagatg tgttcatgac tggtcgcacc
    28081 acaggtgttg caacctatcg taacaacgac ttctttggtc tggttgatgg tctgaacttt
    28141 gctgctcagt atcagggtaa aaatgaccgc actgacgtaa ctgaagctaa cggtgatggt
    28201 ttcggtttct ccactactta tgagtatgaa ggattcggtg taggtgcaac ctatgctaaa
    28261 tctgaccgca ctaatgatca ggttatctac ggtaacaaca gcctgaatgc atctggtcaa
    28321 aatgctgaag tatgggcagc tggtctgaaa tatgatgcga acaacatcta tctggctacc
    28381 acctattctg aaacccagaa catgactgtt tttggtaata accatattgc caacaaagca
    28441 caaaacttcg aagtagttgc acaatatcag ttcgacttcg gtctgcgtcc gtccgttgct
    28501 tacctgcaat ctaaaggaaa agacttgggt gcgtggggtg atcaggacct gattgaatat
    28561 attgatgtag gtgcaaccta ttacttcaac aaaaatatgt ccacttttgt tgattacaaa
    28621 gtcaacctga ttgataagag cgatttcacg aaagcatctg gcgttgctac cgatgatatc
    28681 gttgctgtag gattggttta ccagttctaa tctgattact caaagatatg ttgtgggagg
    28741 ctttgcctcc ccaacatata agtggcgccc tcaagccact ttccttagaa gcactatctt
    28801 gcttctgact atataaatta ccctttattt tctgggacgt ttcaacgccc catttttaat
    28861 aatactcagt gaacaattaa cgtatcaatt agatttaata acaacgatat ccgtacctaa
    28921 cctgatatct aatgtcataa gcatcccttt aattatcccc tcaacctctt gtaaatttcc
    28981 cactcttgag ctccacatag tccaaacgcc ggatgcaaaa aaaatcacac gtgcatctct
    29041 gaaattgtga tggaggaagt gagtatcaat tatatgtgct tataaacgtt cccgattggc
    29101 acagaagtca ccggattgtt caggctccaa taggttgagt atgtcgtatt tattttccaa
    29161 aataaaaatc gatgctgttg ctccttaata aaatactttt actctctgga cgcttttctt
    29221 agctctcttg gtgttatctt aaaacgatta tggaaccttt cagtaaaacg agaaggacac
    29281 ttgtaaccat tttccctggc aatctcgctg atgggtttta ctgtcgtttg tatagcagac
    29341 aacgcattat ttaacctcac gtcgtccaat atactttgaa aacttattcc ctcgc
//
LOCUS       DADMGC010000039        29333 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.039, whole genome shotgun sequence.
ACCESSION   DADMGC010000039 DADMGC010000000
VERSION     DADMGC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.039"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(143..376)
                     /locus_tag="JNZ73_001706"
     CDS             complement(143..376)
                     /locus_tag="JNZ73_001706"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732015.1"
                     /translation="MSKEYTLSVPPFSLNTLGVDMYNNSSYIRKSYIDIPMYNRNLCD
                     FHDLTYSIKSATAILNGKQRDLLKEEHIQVQDK"
     gene            complement(373..1644)
                     /locus_tag="JNZ73_001707"
     CDS             complement(373..1644)
                     /locus_tag="JNZ73_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY8732016.1"
                     /translation="MWSGKVILLSLGLSLASFCHASTDIDQLYERIVNKDQTALKELK
                     KLADTNNTQALVLLGFIYEHGVTVSVDIPKAIQWYEKACNQGGEYGCYNARYFFQYGK
                     GVTLDVTRAQKYASKSKTDDLNIPPDLMNKIINQAYELKSGAETDKTKRASLIEFVSR
                     YVGQAPENDLLFWGRIGFSKPEILRLATLWAQEGDPEMDYIVGNLYLDDFSPSDDRRS
                     LDTIKKALQWFRTAAEKGYPMAQYTMGDTYENGSAGLKIDAVEAKKWYELAANKNDEK
                     ALVALGNIYYSGLTGEVDYSKASMLFDKAEQQGAKKAALWLSWMYYNGLGETLDCDKV
                     WDYYEKGAGKYSMKFNKDEYLTRCNQDQKTRETQGDTLPYITLKHYGTYGFNANEKPK
                     ICDVSIEINADKISSITNLQLTLELKGGGRE"
     gene            2038..3408
                     /locus_tag="JNZ73_001708"
     CDS             2038..3408
                     /locus_tag="JNZ73_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8732017.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFIVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQRVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPENVMADEKLEKCLLTTPGVKDVLLAKLEHCVYVKVDSKITNRFEIERV
                     INEVSY"
     gene            3590..5744
                     /locus_tag="JNZ73_001709"
                     /pseudo
     CDS             3590..5744
                     /locus_tag="JNZ73_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5797..7527
                     /locus_tag="JNZ73_001710"
     CDS             5797..7527
                     /locus_tag="JNZ73_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732018.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSDPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWMKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDEIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(7587..8078)
                     /gene="yajQ"
                     /locus_tag="JNZ73_001711"
     CDS             complement(7587..8078)
                     /gene="yajQ"
                     /locus_tag="JNZ73_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HAY8732019.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            8246..9157
                     /gene="panE"
                     /locus_tag="JNZ73_001712"
     CDS             8246..9157
                     /gene="panE"
                     /locus_tag="JNZ73_001712"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAY8732020.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            9120..9710
                     /gene="yajL"
                     /locus_tag="JNZ73_001713"
     CDS             9120..9710
                     /gene="yajL"
                     /locus_tag="JNZ73_001713"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HAY8732021.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(9764..11212)
                     /gene="thiI"
                     /locus_tag="JNZ73_001714"
     CDS             complement(9764..11212)
                     /gene="thiI"
                     /locus_tag="JNZ73_001714"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAY8732022.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            11418..11660
                     /gene="xseB"
                     /locus_tag="JNZ73_001715"
     CDS             11418..11660
                     /gene="xseB"
                     /locus_tag="JNZ73_001715"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAY8732023.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            11660..12559
                     /gene="ispA"
                     /locus_tag="JNZ73_001716"
     CDS             11660..12559
                     /gene="ispA"
                     /locus_tag="JNZ73_001716"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HAY8732024.1"
                     /translation="MDFPQQFEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            12584..14446
                     /gene="dxs"
                     /locus_tag="JNZ73_001717"
     CDS             12584..14446
                     /gene="dxs"
                     /locus_tag="JNZ73_001717"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAY8732025.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            14501..15475
                     /gene="yajO"
                     /locus_tag="JNZ73_001718"
     CDS             14501..15475
                     /gene="yajO"
                     /locus_tag="JNZ73_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HAY8732026.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEENSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRGDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPMARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(15529..16047)
                     /gene="pgpA"
                     /locus_tag="JNZ73_001719"
     CDS             complement(15529..16047)
                     /gene="pgpA"
                     /locus_tag="JNZ73_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAY8732027.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(16025..17002)
                     /gene="thiL"
                     /locus_tag="JNZ73_001720"
     CDS             complement(16025..17002)
                     /gene="thiL"
                     /locus_tag="JNZ73_001720"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HAY8732028.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDVLSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(17080..17499)
                     /gene="nusB"
                     /locus_tag="JNZ73_001721"
     CDS             complement(17080..17499)
                     /gene="nusB"
                     /locus_tag="JNZ73_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAY8732029.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(17519..17989)
                     /gene="ribE"
                     /locus_tag="JNZ73_001722"
     CDS             complement(17519..17989)
                     /gene="ribE"
                     /locus_tag="JNZ73_001722"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAY8732030.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGSAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(18078..19181)
                     /gene="ribD"
                     /locus_tag="JNZ73_001723"
     CDS             complement(18078..19181)
                     /gene="ribD"
                     /locus_tag="JNZ73_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HAY8732031.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(19185..19634)
                     /gene="nrdR"
                     /locus_tag="JNZ73_001724"
     CDS             complement(19185..19634)
                     /gene="nrdR"
                     /locus_tag="JNZ73_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAY8732032.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            19785..20324
                     /locus_tag="JNZ73_001725"
     CDS             19785..20324
                     /locus_tag="JNZ73_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY8732033.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            20623..21507
                     /locus_tag="JNZ73_001726"
     CDS             20623..21507
                     /locus_tag="JNZ73_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HAY8732034.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(21684..22031)
                     /locus_tag="JNZ73_001727"
     CDS             complement(21684..22031)
                     /locus_tag="JNZ73_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HAY8732035.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(22160..23131)
                     /gene="secF"
                     /locus_tag="JNZ73_001728"
     CDS             complement(22160..23131)
                     /gene="secF"
                     /locus_tag="JNZ73_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HAY8732036.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(23142..24989)
                     /gene="secD"
                     /locus_tag="JNZ73_001729"
     CDS             complement(23142..24989)
                     /gene="secD"
                     /locus_tag="JNZ73_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HAY8732037.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(25017..25349)
                     /gene="yajC"
                     /locus_tag="JNZ73_001730"
     CDS             complement(25017..25349)
                     /gene="yajC"
                     /locus_tag="JNZ73_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAY8732038.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(25372..26499)
                     /gene="tgt"
                     /locus_tag="JNZ73_001731"
     CDS             complement(25372..26499)
                     /gene="tgt"
                     /locus_tag="JNZ73_001731"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAY8732039.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDMHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(26554..27624)
                     /gene="queA"
                     /locus_tag="JNZ73_001732"
     CDS             complement(26554..27624)
                     /gene="queA"
                     /locus_tag="JNZ73_001732"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAY8732040.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            27717..28290
                     /gene="acpH"
                     /locus_tag="JNZ73_001733"
                     /pseudo
     CDS             27717..28290
                     /gene="acpH"
                     /locus_tag="JNZ73_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(28295..>29333)
                     /gene="malZ"
                     /locus_tag="JNZ73_001734"
     CDS             complement(28295..>29333)
                     /gene="malZ"
                     /locus_tag="JNZ73_001734"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HAY8732041.1"
                     /translation="DRHNLGTGGACHNSESPWRDWYLFSDDGTALDWLGYASLPKLDY
                     QSESLVNEIYRGEDSIVRHWLKAPWNMDGWRLDVVHMLGEAGGARNNMQHVAGITEAA
                     KETQSEAYIVGEHFGDARQWLQADVEDAAMNYRGFTFPLWGFLANTDISYDPQQIDAQ
                     TCMAWMDNYRAGLSHQQQLRMFNQLDSHDTARFKTLLGRDIARLPLAVVWLFTWPGVP
                     CIYYGDEVGLDGKNDPSCRKPFPWQVEKQDTALFALYRRMIVLRKKSQALRRGGCQVL
                     YAEDNVVVFVRVLNQQRVLVAINRGEASEVVLPASPFLNAVQWQCKEGHGQLTDGILA
                     LPAISATVWMN"
BASE COUNT         7750 a         7398 c         6923 g         7262 t
ORIGIN      
        1 cagtgtattt ataacatcgg gagcagattt accaccgtat atgctgtcat acaattcatg
       61 cgctttctta cttgccacca cgcctgatga aactatcagc attaaagaaa ataatattct
      121 attaggcaac ataaaatatc tcttatttat cttgtacctg aatatgctct tcttttaata
      181 aatcacgttg ttttccattc agtatcgctg tcgcagattt aatagaataa gtcaaatcat
      241 gaaaatcgca taaattacga ttatacattg ggatgtctat atagcttttt cttatataac
      301 tggaattatt atacatatcc acaccgagcg tattcaaact aaaaggaggg acagaaagag
      361 tatattcttt actcactccc tccccccccc ttttagctca agcgtcaact gcaaattagt
      421 tatactacta attttatctg cattaatttc aatactgaca tcgcatattt tgggtttttc
      481 atttgcgtta aagccatagg tcccgtaatg ttttaacgtg atgtaaggta aagtatcacc
      541 ctgtgtttct cgggttttct gatcttgatt acaacgggtt aaatattcat ccttattgaa
      601 tttcattgaa tatttccccg cacctttttc gtaataatcc cataccttat cacaatcaag
      661 cgtttcacct aatccgttgt aatacatcca acttaaccac aatgcggctt tttttgcccc
      721 ttgctgttca gccttgtcaa aaagcatgct cgctttagaa taatcaacct caccggtcaa
      781 accagaatag tatatattcc ccagggccac taatgctttt tcgtcattct tatttgcagc
      841 taattcgtac cattttttgg cttcgacagc atcaatcttt aaaccagcac tgccattttc
      901 gtaggtatcg cccatggtat actgcgccat cggataacct ttttctgccg cagtacgaaa
      961 ccattgcagt gctttcttta tggtgtctaa agaacgtcta tcatcagaag gtgaaaaatc
     1021 atcgagataa agatttccga caatataatc catttcgggg tcgccttcct gcgcccatag
     1081 agtcgccaga cgcagaattt caggtttact gaaaccaatt cgcccccaaa aaagcaaatc
     1141 attctcgggt gcctgaccga cataacgact gacaaattca attaaactcg cccgtttcgt
     1201 tttgtctgtc tcagccccgg atttcaattc ataggcctga ttgattattt tattcatcaa
     1261 atcaggcgga atattaagat cgtcagtttt cgacttactg gcatacttct gtgctcgtgt
     1321 cacatctagg gtgacgcctt taccatattg aaagaaataa cgtgcattat aacaaccgta
     1381 ttcgcccccc tggttacagg ctttctcata ccactgaatc gcctttggga tgtcaacaga
     1441 tactgtcact ccgtgttcat aaataaatcc cagtagcact agcgcctgcg tgttattggt
     1501 atcagctaat tttttcagtt cttttaacgc cgtctgatct ttattgacaa tacgttcata
     1561 cagttgatca atatctgttg aagcatgaca aaaagaagcc agagataaac ccaatgataa
     1621 taaaataact ttaccagacc acatacattc ctccttgata tattcaacca ccaaacatcc
     1681 acctcatgaa taaatacgct gccataacgg cccaggtaat aagtaaacat ttaagctgaa
     1741 acagcataac cagtgcctcc aacgcttcta tttattgcga taaaataaca ccatcctttg
     1801 ataaaaagtc acacgcccat caataaatcc aaaaataatg tcattagttg caggataaaa
     1861 tcatttgagc ttttcatttg ttatatttaa cgcatgatgt attaatggca agaatatgag
     1921 agaaactcat acctgacgta aaaatcactc tcttatgtaa tatttactta ttaacactct
     1981 gtttacctaa catgcgctcc gcactacact atgtgggcaa ttttttaaga ggtggtaatg
     2041 aacgattata aaatgacgcc aggtgagcgg cgcgcgacct ggggtttagg gaccgtattt
     2101 tcattgcgca tgctgggcat gtttattgta ctgccggttc tgactacgta cggtatggca
     2161 ctgcaaggcg ccagcgaagc attaatcggt attgccattg gtatttatgg tctgacccag
     2221 gccgtttttc agattccgtt tggcctgctt tccgaccgca ttggtcgaaa accattaatt
     2281 gtcggtgggc tggcagtgtt tgccgccggt agcgttatcg ctgcgctttc tgactccatc
     2341 tggggaatta ttctgggccg ggcgctacaa ggctccggtg cgattgccgc tgccgttatg
     2401 gcgctgcttt ccgatctcac gcgcgaacaa aaccgtacca aagcaatggc gtttatcggc
     2461 gtgagctttg gcattacctt tgccattgcg atggtgcttg gcccgatcat cactcacaaa
     2521 cttgggctgc acgcgctgtt ctggatgatc gctattctgg caacgaccgg cattgcgttg
     2581 accatttggg ttgtgcccaa cagtagcact cacgtactta atcgtgagtc cggaatggtg
     2641 aaaggcagtt tcagtaaagt gctggcggaa ccgcggctgc tgaaactcaa ctttggcatt
     2701 atgtgtctgc atattttgct gatgtcgacg tttgttgccc tgcccggaca actggctgat
     2761 gcggggttcc cggcggctga acactggaag gtctatctgg cgacaatgct aatcgccttt
     2821 ggctcggtcg tgcctttcat tatctacgct gaagttaagc gcaaaatgaa gcaagtcttt
     2881 gtcttctgcg tcgggttgat cgtggttgcg gaaattgtgt tgtggaacgc gcaaacgcag
     2941 ttctggcaac tggtggtcgg cgtgcagctt ttctttgtgg cgtttaattt gatggaagcc
     3001 ctgctgccct cacttatcag taaagagtcg ccagcaggtt acaaaggtac ggcgatgggt
     3061 gtttactcca ccagccagtt tcttggcgtg gcgattggcg gttcgctggg cggctggatt
     3121 gacggcatgt ttgacggtca gcgggtattt ctcgctggcg caatgctggc cgcagtgtgg
     3181 ctggcagtcg ccagtaccat gaaagaaccg ccgtatgtca gcagtttgcg cattgaaatc
     3241 ccagaaaacg taatggcaga tgaaaaactg gaaaaatgct tactgacaac gcctggtgtc
     3301 aaagatgtac tgctggcgaa gttagaacat tgcgtctatg tgaaagtaga cagtaaaatc
     3361 accaatcgtt ttgaaataga gcgagtaatc aacgaagtat cttattaaat aataagcacg
     3421 gtgctattca tgaaaaataa aatagcaccg tacttcgtaa caccataatc ctttaaaccc
     3481 tccattatta atacttcgct taattccata taactcgacg attacattag aagtaaacta
     3541 tactttttat gaaatatcag ggataaatta ttcacatgga aaatacatca tgtacaaatc
     3601 attatttact attatcattc tactgtatag cgctagttca tatgcaaaaa gcgaatcccc
     3661 tcccgcaagc actataactt tcattgcaca aatcgatcat gttgagctaa ataaaacagc
     3721 attatctcat gatatgaaac tcgttcttgc cgatcgtttt tatttcaaaa ccagtaatcc
     3781 ttgcgaaaca aattcaattt ctgctcgacc agaatcaatg gatattactg acaaagccta
     3841 tgccaacaaa ttaaaaaaat tagaaactcc ttttcttgat gatcgctatt tctttatgac
     3901 catcaatcag tgtgacggta atggcactcc catgctgaca gacataaaag tatgtacaac
     3961 agaattatgt ggtgctgaat atacaaagaa aatgtctact gtatggctcg atgaaacttt
     4021 aaaaccaact gctaaacgtc tggcaattta ttttattcaa tctcctttac cctatgacga
     4081 ggagaaaaaa caatggaaag tcaccggatg gtatatctca tcaactgata atcagtcttc
     4141 aactaaacag attgcatttg aaggatatac agacacagat aacttctccg attctaaatt
     4201 tatttcttca tttaaatcct ggtttgaaag tggaaagata aaaaacattg ctaactataa
     4261 tccaaaagga gaaagagatg gaaaatacga aagttattac aatagcaatg gtaacgtcta
     4321 tgaatctcta agtttttcta atggtcttat caacggtgag tttattacat attatgaaaa
     4381 tggggccatt gaaagtaaac gtcattttaa taacgacaaa attaaagatg gtgaatgccc
     4441 tcatttttat gaagatggga aaatcaaaca gcaacatagc tatctgaata gtaaactcga
     4501 tggtcctgca tatgaatatt atcctgatgg caaactcatg caggaaaatt tctatcagca
     4561 aagtgtgttg attggaaacg ataccagtta ctaccaatcc ggaaaaataa tctctataca
     4621 caatagaaat tcccgtggtc agtatgatgg agctaacgaa cgttatagtg aagagggtaa
     4681 gttacagtct aaatccgtct ataaagacgg aaaacaaatt tctgttcaaa cctggtatga
     4741 aaatgggcag aaagaagagg agaaacattt tgatgagcaa ggccaattaa atggtctggt
     4801 taagcaatgg tataaaaatg gtaatcttgc aaaatcacaa aactataaac atgacatact
     4861 cgatggtaac agtgaagagt ggtacgaaaa tggcattcct gaaagtttgt acccttataa
     4921 gaatggaaaa accgacggca ttgccaaatc atggaataag tatggtaaat taacctattc
     4981 aatcgaatac aaaaatagcg tagagaatgg ggcatatcgt aactggagca aaaacacagg
     5041 taaactaacc aaagaaaccc tgtacgtcaa tggtatcaga cagggagtgg aaaaagagtt
     5101 taacgatcgc acaggtaaac tattaacctc cacgcaatat gttaataata aaagacatgg
     5161 tacggaggaa acttatgatc aaaacggaat aaaatatatt acatgctatc aaaatgatca
     5221 gaaattatcg tctttagaca atccaacaca aataaaagat aatgcaacaa caggcgattc
     5281 cagtgctcaa tttgcattag ggaaatacga attcatctgt gcaaatatag atgaagggat
     5341 taaatggtta accaaatctg cagagcaaaa aaaacactga tgcgatatac tttttagcta
     5401 cagcctacaa aggcaatggt atacctgcaa acaatgaaaa atacctagca tatctgcaac
     5461 aagctgcaac actgggcagt agtaatgccc aggcggaaat tggctattta tatctcatcg
     5521 gaaaagaact accacagaat cttcctgatg ctggtgtctg gttcaaaaaa gcagctgcac
     5581 aaggtaattt tgccgcacat ttctatctgg gaaggatgta tcagaatggt gacggcgttg
     5641 agagaaatat ggaaaaagcc aggtttcatc tatctaatgc ggcagaaggt ggaatagaac
     5701 ctgcgcttaa agcattaaat gaacttgaac atcaaacaaa ataaaacgct cagggaccac
     5761 gtaaggttca tcaaacagaa aaggatatag tttgcgatga aagcgaaatc tactttgtta
     5821 ttaatgattt tatctgtttt ttattctcat caggttttat ccagcgaccc cactcagccg
     5881 gatgtttatt ctgtcgtcga gaaaaaacta aactctgctt tacctctgga agaaaatacc
     5941 accttcagat cgcaggctga atggtttaat ttgcaatatg aattattagt atctggctac
     6001 cctgaacgtg cttacaattt actgagcaaa cttgagacag aatcgaaaaa cgctaaatat
     6061 tatctggatt taactgcctc cagagtcgct gaagaagaag caccagagac agccttaact
     6121 tttttatcaa aaattgggtt aaataaaccg gctactctta catcctttga gagctatatc
     6181 aatagctgga tgaaaagcaa acaacccgaa aaggctatgc agcttctcag cctcgatgag
     6241 aatgccctga attattattt accgactgtg ttagtcgcat atcagatcgt tcctgataag
     6301 gcaataagta tttataattc tgtatatgga gataatgttg ttatacccta tcagcaagtc
     6361 accatgttac ttacagttgc ggagggttat caaacaaaag gcgatataaa aaacgccatt
     6421 cattatgccg ataaggcgct gaatatgttt gataaagcta ttgctgtaga tagcagtcgt
     6481 gaatcccatt atagtgatga gtatttgaaa ctcatgaata tctacgccgc caatggcaac
     6541 aaagaaaaag cggtggtttt atctcaacgt ctacacaaag ccattaccga atctcaatcg
     6601 aattacttaa gtactctgga aggaatttta ttgttttata gaaataatga tatgcagcaa
     6661 gactatcaaa acagcctttc aaattacata gtatttatcg atgaaatttt tgccttttcc
     6721 ccatcaacaa gaagtgaatt aagcctcatt aatttattaa attcattaaa tgaggtggaa
     6781 ttgatgagaa aacgtctggc gtttctgatg acagcaccag aatacgcttg ttatgatagt
     6841 cagtattgtt atgaagacaa agtcagcgca ttaaaaatcc tgtatcgtca tcatgacaat
     6901 gagctaatgg acaaatattt cacgactctt cttaaagaga tcagtaatca accattagaa
     6961 gactgggata taataataag taatactggt gaaaaattta gtgaagtggg tctaaccaaa
     7021 tatgctcaac atctcgcatc tttcgctgaa gaattttata taaaagagca aaaatcacat
     7081 tctgaaagtg aaatgagagg actattttca tcacttgccg aattatatag ttttggtaat
     7141 gatactgcca gcgcaaaccg agtgttccat cagcatgtcc ctgccttcaa tacagatcat
     7201 atgattcaat attttataaa tgccaaagaa tggaacaatg ccagggaatt attgataaaa
     7261 gaggaaatgt tgggtatgca taatctgata ttgttggaga atatttgtat gcaaaaaaat
     7321 gcagaatgca tggcacacat tacctttacg cttaataaat taacaacaca accagcaata
     7381 acaaaaatag atagcgtggg taatgagcaa ctttatcaga ttggcaaaat ctaccataag
     7441 ttggaaatta aacctgagcc agagcaacag gttttaattc agtcgcttta tgaccgggca
     7501 tcaggaagcg cctctgcaac tcaataaatg catggtcaaa agggccacca cggtggccct
     7561 gacaatcagt agctatatcc agaaagttaa tcgcggaagt ttttgaactg gaacggctga
     7621 ccgagatcgc caccgcgcac catcgccatg acagcctgca aatcatcacg agatttgcca
     7681 gtcacgcgga tctcatcgcc ctgaatttgc gcctgcactt tcagtttgct gtctttgatc
     7741 atcttgacga tttttttctg agtggcgctc tcaatgccct gtttcagttt cgcttccaca
     7801 aaccaggttt taccgctatg aacgatattt tccggtacat ccagtgaact gccttcaatg
     7861 ccgcgcttca gcagcttggc acgcagaata tccagcaact gattgacctg gaagtcggac
     7921 tcgctcaaca ctttgatggt tttgctggcg tcgttcagct caaatgaggc ttcaacgtta
     7981 cggaagtcaa aacgggactc cacttcgcga ctcgcgttat cgaccgcgtt acgtgcttcc
     8041 tgaagatcaa cttcagagac aatatcgaaa gatggcatct tttcctctcc cttcattttt
     8101 gatgcgaagc ataatacctg caaagttaaa taaccaacag tcctgcacca ggacgtatcc
     8161 cttaattgtg catagtcgca acagcatgca caattaaggg atagccttat tgaacacccg
     8221 gagtggttgt gggtgaggag gaacaatgaa aattaccgta ttgggatgcg gtgccttagg
     8281 gcaattatgg cttacagcac tttgtaaaca gggtcatgaa gttcagggct ggctgcgcgt
     8341 accgcaacct tattgtagtg tgaatctggt tgagacagat ggttcgatat ttaacgaatc
     8401 gctgaccgcc aacgatcccg attttctcgc caccagcgat ctgctcctgg tgacgctgaa
     8461 agcatggcag gtttccgatg ccgtcaaaag cctcgcatcc acactgcctg taactacgcc
     8521 aatactgtta attcacaacg gcatgggcac catcgaagaa ttgcaaaaca ttcagcagcc
     8581 attactgatg ggcaccacca cccatgccgc ccgccgcgac ggcaatgtca ttattcatgt
     8641 ggcaaacgga atcacgcata tcggtccggc acggcaacag gatggcgatt acagttatct
     8701 ggcggatatt ttgcaaaccg tgttgcctga cgttgcctgg cataacaata ttcgcgccga
     8761 gctgtggcgc aagctggccg tcaactgcgt gattaatcca ctgactgcca tctggaattg
     8821 cccgaacggt gaattacgtc atcatccgca agaaattatg caaatatgcg aagaagtcgc
     8881 ggcagtgatc gaacgcgaag ggcatcatac ttcagcggaa gatttgcgtg attacgtgat
     8941 gcaggtgatt gatgccacag cggaaaatat ctcgtcgatg ttgcaggata tccgcgcgct
     9001 gcgccacacc gaaatcgact atatcaatgg ttttctctta cgccgcgccc gcgcgcatgg
     9061 gattgccgta ccggaaaaca cccgcctgtt tgaaatggta aaaagaaagg agagtgaata
     9121 tgagcgcatc ggcactggtt tgcctcgccc ctggtagtga agagactgaa gccgtcacca
     9181 cgatcgacct cttggttcgc ggcggtatca aagtcaccac tgccagcgtc gacagcgatg
     9241 gcaacctggc aattacctgc tcgcgcggcg tgaagctgct ggcggatgcg ccgctggtcg
     9301 aagtggctga tggcgaatat gacgtgatcg tgctgcctgg tggcattaaa ggcacggagt
     9361 gttttcgcga tagcactctg ctggttgaaa ccgttaaaca gttccaccgt tccgggcgta
     9421 tcgtcgcggc tatttgcgcc gcgccagcca ccgtgctggt gccgcacgat atctttccga
     9481 ttggcaatat gaccggcttc ccgacgctga aagacaaaat tcccgctgaa caatggcagg
     9541 acaagcgcgt cgtctgggat gcacgggtaa aattgctgac cagccagggg ccgggtacag
     9601 ctatcgactt tggtctgaaa attatcgacc tgctggttgg gcgcgagaaa gcccatgaag
     9661 tggcatcaca actggtgatg gcggcaggga tttataatta ttacgagtag tgtcggatgc
     9721 ggcaaacgtc gcatctgacc agatgcgacg taaaaactac aaattacggg cgatatacct
     9781 tcacattgtt aaagccttgc tcgcgcagat agagcgcctg cagacggctc atcaccccgc
     9841 gctcacacca cagcagccag gttttgttct ggtcgagatc gccaaatttg gtgctcagtt
     9901 tatagaacgg cagagaaacc acatcaatcc cttcgacttt cagtggctta tcttcctgtt
     9961 catcgacaga acggatatcg agaatcacgt cgttcgggcc gaagccgttg acggtttcca
    10021 cttccaccac ttcctgctcg gtctgctggg cgatttcgcg gatatcaacg ttattcgctt
    10081 cctcaaccac tttatcgaga atactgaagt cgaacttctc ttcttccgct tcaatcttcg
    10141 atttaaccgc tttcaccgtc gggcttttgg agatcacgcc acaatattcc ggcatcgtgc
    10201 gagcaaagtc ttcggtgcca atctggcgag ccaggttgat gatgtgctct ttgtcgtaag
    10261 aaatcagcgg acgcaggatc agcgtgtcag agacgttatc aatcaggcgc aggttggtca
    10321 gcgtctgact ggacacctgg ccgagcgctt cgccggtgac cagcgcctgt acgccgtaac
    10381 gttcagccac tttagatgcg gcacgcacca tcatacgttt gaggataacg cccatctgac
    10441 cgtcgtcgat tttctcgaga atttccccga caaccggttc gaaattaatg gcgacaaaac
    10501 gcacgcggtg ggagctgcca aagcggttcc acagataatg cgccacctga cgcacgccaa
    10561 tttcatgcgc tgcgccgccg aggttaaaga agcagtaatg cacgcggcag ccgcgacgca
    10621 tcaacatata actggaaaca ccggagtcga aaccaccgga aatgagcgac agcacatctt
    10681 cctgggtgcc aatcgggaaa ccaccaatac cttcgtagcg acctttaatc agcaggaggc
    10741 gatcgtcttc cacttccaga tgaaccgtta catccgggtt agtcagcttc acgcgcgcgg
    10801 attcaatatg ctgatttaaa ccgccgccga cgtaacgttc cacatcaatc gagctaaaat
    10861 catgtttgcc acggcgcttc acgcgtacgc agaaggtttt gccttccagc tgatcgcgat
    10921 actgaaccaa cgctttctca aaaatatcgt gcatgtcggt aaacggcacg tcttcgactt
    10981 cgagaatatg gtggataccc ggaatacggg tcagggcgtc gcgaatagtc agacgctggt
    11041 tttcatcttt cgcgcgaact tcgatgttat cccagtggcg gacgacagcg agcgtctcat
    11101 catagtgctt taaaacgtta cgaatgttcc cggtaaggat ttttataaag cgcaagcgca
    11161 cagattggct tttgatggtg atttccggga acaatttaat gataaacttc atggcggcaa
    11221 tggttcgttg gcaagcctta agcgacttgt atagggaaaa atacagcagc ccacacctgc
    11281 ggctgcatcc aggcgcggaa gtataccact aacatcgctt tgctgcgcac atcaccttac
    11341 cattgcgcgt tatttgctat ttgccctgag tccgttacca tgacggggcg aaaaatattg
    11401 agagtcagac attcattatg ccgaagaaaa atgaggcgcc cgccagcttt gaaaaggcgc
    11461 tgagcgagct ggaacagatt gtaacccgtc tggaaagtgg cgacctgccg ctggaagagg
    11521 cgctgaacga gttcgaacgc ggcgtgcagc tggcacgtca ggggcaggcc aaattacaac
    11581 aagccgaaca gcgcgtacaa attctgctgt ctgacaatga agacgcctct ctaacccctt
    11641 ttacaccgga caatgagtaa tggactttcc gcagcaattc gaagcctgcg ttaagcaggc
    11701 caaccaggcg ctgagccgtt ttatcgcccc actgcccttt cagaacactc ccgtggtcga
    11761 aaccatgcag tatggcgcat tattaggtgg taagcgcctg cgacctttcc tggtttatgc
    11821 caccggtcat atgtttggcg ttagcacaaa cacgctggac gcacccgctg ctgccgtaga
    11881 gtgtatccac gcttactcat taattcatga tgatttaccg gcgatggatg atgacgatct
    11941 gcgtcgcggt ttgccgacct gccatgtgaa gtttggcgaa gcaaacgcga ttctcgctgg
    12001 cgacgcttta caaacgctgg cgttctcgat tctaagcgat gccaatatgc cggaagtgtc
    12061 ggatcgcgac agaatttcga tgatttctga actggcgagc gccagcggta ttgccggaat
    12121 gtgcggtggt caggcattag atttagacgc ggaaggcaaa cacgtacctc tggacgcgct
    12181 tgagcgtatt catcgtcata aaaccggcgc attgattcgc gccgccgttc gccttggtgc
    12241 attaagcgcc ggagataaag ggcgtcgtgc tctgccagta ctcgacaagt acgcagagag
    12301 catcggcctt gccttccagg ttcaggatga catcctggat gtggtaggag atactgcaac
    12361 gttgggaaaa cgccagggtg ccgaccagca acttggtaaa agtacctacc ctgcacttct
    12421 gggtcttgag caagcccgga agaaagcccg ggatctgatc gacgatgccc gtcagtcgct
    12481 gaaacaactg gctgaacagt cactcgatac ctcggcactg gaagcgctag cggactacat
    12541 catccagcgt aataaataaa caataagtat taataggccc ctgatgagtt ttgatattgc
    12601 caaatacccg accctggcac tggtcgactc cacccaggag ttacgactgt tgccgaaaga
    12661 gagtttaccg aaactctgcg acgaactgcg ccgctattta ctcgacagcg tgagccgttc
    12721 cagcgggcac ttcgcctccg ggctgggcac ggtcgaactg accgtggcgc tgcactatgt
    12781 ctacaacacc ccatttgacc aattgatttg ggatgtggga catcaggctt atccgcataa
    12841 aattttgacc ggacgccgcg acaaaatcgg caccatccgt cagaaaggcg gtctgcaccc
    12901 gttcccgtgg cgcggcgaaa gcgaatatga cgtattaagc gtcgggcatt catcaacctc
    12961 catcagtgcc ggaattggta ttgcggttgc tgccgagaaa gaaggcaaaa atcgccgcac
    13021 cgtctgtgtg attggcgacg gcgcgattac cgctggcatg gcgtttgaag cgatgaatca
    13081 cgcgggcgat attcgtcctg atatgctggt ggtcctcaac gacaatgaaa tgtcgatttc
    13141 cgaaaatgtt ggcgcgctta acaaccatct ggctcagctg ctttccggta agctttactc
    13201 ttcactgcgc gaaggcggga aaaaagtttt ctctggcgtg ccgcccatta aagagctgct
    13261 caaacgtacc gaagaacata ttaaaggcat ggtggtgcct ggcacgttgt ttgaagagct
    13321 gggatttaac tacatcggcc cggtggacgg tcacgatgtg ctggggctta tcaccacgct
    13381 aaagaacatg cgcgacctga aaggcccgca gttcctgcat atcatgacca aaaaaggtcg
    13441 tggttatgaa ccggcagaaa aagacccgat cactttccac gccgtgccta aatttgatcc
    13501 ctccagcggt tgtttgccga aaagtagcgg cggtttgccg agctattcaa aaatctttgg
    13561 cgactggttg tgcgaaacgg cagcgaaaga caacaagctg atggcgatta ctccggcgat
    13621 gcgtgaaggt tccggcatgg tcgagttttc acgtaaattc ccggatcgtt acttcgacgt
    13681 ggctattgcc gagcaacacg cagtgacctt tgccgccggt ctggcgattg gtggatacaa
    13741 acccattgtc gcgatctact ccaccttcct gcaacgcgcc tatgatcagg tgctgcatga
    13801 cgtggcaatt caaaagctac cggtcctgtt cgccattgac cgcgcgggca ttgttggtgc
    13861 tgacggtcaa actcaccagg gcgcttttga tctctcttac ctgcgctgca taccagaaat
    13921 ggtcattatg accccgagcg atgaaaacga atgtcgccag atgctctata ccggctatca
    13981 ctataacgat ggcccgtccg cggtgcgcta cccgcgcggc aacgcggttg gcgtggaact
    14041 gacgccgctg gaaaaactac caattggcaa aggcattgtg aagcgtcgtg gcgagaaact
    14101 ggtgatcctt aactttggta cgctgatgcc agaagcggcc aaagtcgccg aatcgctgaa
    14161 cgctacgctg gtcgatatgc gttttgtgaa accacttgat gaagcgttaa ttctggaaat
    14221 ggccgccagc catgaagcgc tggtcaccgt agaagaaaac gccattatgg gcggcgcagg
    14281 tagcggagtg aacgaagtgc tgatggccca tcgtaaacca gtacccgtgc tgaacattgg
    14341 cctgccggac ttctttattc cgcaaggaac tcaggaagaa atgcgcgccg aactcggcct
    14401 cgatgccgcc ggtatggaag ccaaaatcaa ggcctggctg gcataatccc tactccactc
    14461 ctgctatgct taagaaatta ttcatggcag gagtggcagc atgcaataca accccttagg
    14521 aaaaaccgac cttcgcgttt cccgactttg cctcggctgt atgacctttg gcgagccaga
    14581 tcgcggtaat cacgcatgga cactgccgga agagaacagc cgtcccataa ttaaacgcgc
    14641 gctggaaggt ggcataaatt tctttgatac cgccaatagc tattccgatg gtagcagcga
    14701 agagatcgtt ggtcgcgcac tgcgggattt cgcccgtcgt gaagacgtag tcgttgccac
    14761 caaagtgttc catcgcgttg gtgatttacc ggaaggatta tcccgtgcac aaattttgcg
    14821 ctctatcaac gacagcctgc gacgtctcgg catggattat gtcgatatcc tgcaaattca
    14881 tcgcggggat tacaacacgc cgatcgaaga gacgctggaa gccctgaacg acgtggtaaa
    14941 agccgggaaa gcgcgttata tcggcgcgtc atccatgcac gcttcgcagt ttgctcaggc
    15001 gctggaacta caaaaacagc actgctgggc gcagtttgtc agtatgcagg atcactataa
    15061 tctgatttat cgcgaagaag agcgcgagat gctgcctctg tgttatcagg agggcgtggc
    15121 ggtgattccg tggagcccga tggcgcgggg gcgactgacg cgtccatggg gagaaactac
    15181 cgcacgactg gtgtctgatg aggtggggaa aaatctctat aaagaaagcg atgaaaatga
    15241 cgcgcagatc gcagagcggt taacgggcgt cagtgaagaa ctcggtgcaa cacgagcaca
    15301 agttgcgctg gcctggttgt taagtaaacc gggcattgcc gcaccgatta tcggaacttc
    15361 gcgggaagaa cagcttgatg agctgctgaa cgcggtggat atcactttga agccagaaca
    15421 gattgccgag ctggaaacgc cgtataaacc gcatgcggtc gtaggattta aataagagtc
    15481 caggcctgat gagacgtgac aagcgtcaca tcaggcatcg gtgcacaact acgacagaat
    15541 acccagcggc cagtgatgac cgataaagta caggatgcct gcggaaatca ccccggcgac
    15601 aatatcgtcg atcatgatcc ccatgccgcc atgtacattg cgatcaaacc agcggatcgg
    15661 ccacggcttc cacatatcca gaatacggaa aatcacaaac ccggcggcaa cccactgcca
    15721 gtcattggtc ggcagcgcca tgagcgtgat ccacatacca ataaattcgt cccagacaat
    15781 gctgccgtga tcgtgcacgc ccatatcttt cgccgtttga tgacaaagat agacgccgat
    15841 acagatcccc aacatcacca ccagcgagta gagctgccag ggtaaaaagg tcatcagata
    15901 ccagaaaggg atcgccgcca gcgatcccat cgtgccagga acgattgggc ttaatccact
    15961 tccgaatccg acagcaagca gatgccacgg attactcatc ttcaggcgac ttttcgcgac
    16021 atctttatgg cgtggcaaaa tggtcatatc ctttccagtc aaacgtgaca ggttcgccgt
    16081 cacgaataaa acaaagccct tcgatatcgg cggtcatttg cccgatacag gtaaacggta
    16141 cgccaaggtg tccgagagca acatccagcg cgccacggtt cagttccggc accgtgaaac
    16201 acaactcgta atcttcaccg ccagagagcg cccagcgcag cgcctgttcc ggttcaacat
    16261 ggcgagaaag cacatcagaa aacggcagca atgccaggtc aatacgtgcg ccgcagtcgc
    16321 tggctttcac gatatgcccg agatcggaaa tcagaccgtc agagagatcg atagctgaat
    16381 ttgccagatc gcgcagtgcc tgcccctgta aaatacgcgg cgatggacgg agatgacgtt
    16441 tgatcaagta gtcagcatct ttagcatcgg caacctgcaa acggttttgc aaaatcgcca
    16501 gcccggcggc gctatcgccc ggtgtaccgg tcacatagat ccagtcaccc ggtttcgccc
    16561 cagagcgcgt taaggctcgt cccatcggaa caaagccgtg gatacccaac gtcattgata
    16621 atggcccacg cgtggtatcg ccgccaatga gttgcatatc gtaataattg agaagatcaa
    16681 acaaactgtc gctgaaggac tcaagccacg cttcgtctac gtccggtaag gttaatgcca
    16741 gcgtcagcca ggccggatcg gcccccatcg ctgccagatc gcttaggttc accgccagtg
    16801 ctttataagc cagatcagca ggatcgatat cagggaggaa atggttgccc gccaccagcg
    16861 tatcagtgct gatcgccagg gtctgtttct cggggatatt gagaagagcg caatcgtcgc
    16921 cgatgcccag ttcgacatca agacgagaac ttcttacacg gtcaaaataa cgggcaatca
    16981 gggagaactc gccacatgcc atacgttatg cctcagcagg taaaaaagaa aaggccggcg
    17041 atcatggaac agtcttccgt gaatctaccg gcctggatat tactttttgt tagggcgaat
    17101 cacaggtgct gctttatcga gtacgccgtt gacgaactta tggctgtctt ctgcgccgaa
    17161 cgatttcgcc agttcgatcg cttcgttaat ggccactttg tatggcacat cactacgttt
    17221 agacagttcg tacagcgcaa tgcgcagtac tgctttttct acctggccca gttcttccag
    17281 caggcgggac aggtagggct tcatcagtcc gtcgaggtat gcggtgttag tcgccacccc
    17341 ggccagcagc tcacggaagt acagaacgtc aacgtctttt acatcctgtt cagccaggaa
    17401 ctggtattca acatcagcga tgtcgttctg ggacaactgc caggagtaga gcgcctggac
    17461 ggcacactca cgagcgcggc gacgagcagc aggtttcacg gatttcccct tactaatttc
    17521 aggccttgat ggctttcaat acattaatca tttcaagcgc ggtcagtgca gcttctgcac
    17581 ctttgttgcc agctttggtg ccagcacgtt cgatcgcttg ttcaatgctt tcagtggtca
    17641 gaaccccaaa agcaaccgga atttcgctgt cctgggcaac atgcgccagg ccgttgcttg
    17701 caccaccagc gacatattca aagtgggcag agccaccacg aataaccgta cccagcgcaa
    17761 tcaccgcgtc gtatttaccg gttttagcca gtgcacccgc cgccagcggc agctcatagg
    17821 caccaggcac ccaaacaacg gtaatgtttt catcttttac ctgaccgata cgtttcagtg
    17881 cgtcaattgc accttccagc aggctgtcat tgataaagtt gttgaaacgc gcgatggtga
    17941 tggcgacgcg agcgtccggg gtagcaacgt tagcttcaat aatgttcatt ttcttccttc
    18001 gggttcgagt atggccccgc aggggggcgg attttagcat aatatttcgt gcgctgcttc
    18061 cctttcgagc cgggagatca tgcccccact aaatgcaggc aaacatccgg gcctacatga
    18121 cgtatctctt tgaatttaaa ttggggggcg tcggctaatt tctcaagccc tggcagcgag
    18181 cataatccac gggcgtcgct gcctaatagt ttaggtgcga tatagacaat cagttcatcg
    18241 actaaacccg cctgtagcaa tacgccagcg agcgttggcc ccgcttccac ccagatgctg
    18301 ttaatctgct gtttacccag ttgcatcatc agtacaacca gatccagatg acctttatgc
    18361 tctggaatca gcaaggtacg caccgtttcc ggccactcac gagaatcttc ctgcgtacgc
    18421 gcgaaccagg tttcgccggg ctgctgcaca atgcgatgtt ccggcgtcac gcgattttgg
    18481 ctatcaatca caatacgtac tggctggcgg agattttgtt gcgggtagag cgcctgagtt
    18541 tgttcatcca gttcagacca acgcactgtt aaggctggat catccgccag caccgtggcg
    18601 ctgctggtta aaatggcatg actttgtgcg cgctgtcgtt gtacgtcgcg ccgcgcctga
    18661 ggcgaagtga tccactggct ttcgccgctc gccatcgccg tgcggccatc aagcgatgcg
    18721 ccaagtttta actgaatata aggaaagccg gtgcgcatcc gcttgagaaa gcctttattc
    18781 aattgctcgg cttcactcat catcagcccg tggctgacgt caatgccagc ctgttgcaga
    18841 cggtaaagtc cacgcccagc gacctgcggg ttaggatctt gcatcgctgc gaccacgcgc
    18901 gctacgccag cggcgatgag tgcgtcacag cacggtggcg tacggccatg atggctacag
    18961 ggttcgagtg tcacatacgc cgttgcgcct ttggcttttt cacccgccat acgcaacgcg
    19021 tgtacttcgg catgtggttc acccgctcgt tggtggtaac cttcaccgac aatttcgcca
    19081 tctttgacaa tgacgcaccc gacattcggg ttggggtggg tggtaaaacg tcctcgttgc
    19141 gccagcttta gcgcccgcgc catgtaatac tcgtcctgca cggcttagtc ctccaggcgc
    19201 gcgatctctt cgccaaattc tttgatatct tcgaaactgc ggtagacaga ggcaaaacgg
    19261 atataggcga ctttatcgag ctttttcaat tgctccatca ccagattgcc aatcatcttg
    19321 ctcggtactt cgcgctcacc ggtggcgcgc agctgcgatt taatatgatt gattgccatt
    19381 tcaacgtaat cggaactcac cggacgtttt tccagcgccc gcagcattcc gctacgcaat
    19441 ttctcttcat taaacggttc acgcacgtcg ttgcttttta caacacgcgg cataaccagc
    19501 tccgccactt caaaggtggt gaaacgttca ttacacacca gacactgccg acggcggcgt
    19561 acggatgaac cctcgcccac gagacgagag tcaattacct tagtgtccac ggcgaaacag
    19621 aatgggcaat gcatacggcg tcctgaccag gtggttaacg gaaatctatt ttaccctgaa
    19681 ctgacgatac aacaaaggcg caacgctttt gagataaagg atcgatgctt tcgctgcttt
    19741 taccctctac cattaggcta attgcgacga gaaagggacg acctatgaat actaacgttt
    19801 ttcgactgct cctgctggga agcctgttca gccttagcgc ctgtgtgcag caaagtgaag
    19861 tgcgacagat gaaacacagc gtcagcacgc tgaaccagga gatgacgcaa ctcaatcaag
    19921 aaacagtcaa aatcactcag caaaacaggc tgaatgcaaa atccagcagt ggggtttacc
    19981 ttctgcctgg agcgaaaaca ccggcaagac tggaaagcca gatcggtact ttacgtatgt
    20041 cgctggtgaa tattacgcct gatacagatg gcaccaccct tacactacgt attcagggtg
    20101 agtcgaatga tcccttgccc gcattcagcg gaactgttga atatgggcaa attcagggaa
    20161 caatagacaa ctttcaggaa atcaatgtgc agaatcaatt gattaatgcc cctgccagcg
    20221 tcctcgcccc cagcgatgtt gatattccgt tacaattaaa ggggatttct gtggatcagt
    20281 taggctttgt acgtatccac gacattcaac ctgttatgca gtaaacgtat tttgggacga
    20341 ttttgtgcgt cccgcaacat ctttccccgt cattttgtta ctctgcttac atcacttgga
    20401 ttgatagtaa aagtttgcaa caagggcgaa agtcagtaca atccccgccc gaatgtgtgt
    20461 aaacgtgaac gcaatcgatt acgtaaatga tagaactgtg aaacgaaaca tatttttgtg
    20521 agcaatgatt tttataatag gctcctctgt atacgaaata tttagaaacg caatttgcgc
    20581 ctttttcact cccgcaaggg attttcaaac agtggcatac atatgaaaaa aacattactg
    20641 gcagccggtg cggtactggc gctctcttcg tcttttactg tcaacgcagc tgaaaacgac
    20701 aaaccgcagt atctttccga ctggtggcac cagagcgtta acgttgtcgg aagctatcac
    20761 acccgtttcg gaccgcagat ccgcaacgat acctaccttg agtacgaagc attcgctaaa
    20821 aaagactggt tcgacttcta tggttatgcg gatgcgccgg tattcttcgg cggtaactcc
    20881 gatgctaaag gtatctggaa ccacggttct ccgctgttta tggaaatcga accacgtttc
    20941 tccatcgaca agctgaccaa tactgacctt agcttcggtc cgttcaaaga gtggtacttc
    21001 gcgaacaact acatttacga catgggtcgt aataaagatg gtcgccagag cacctggtac
    21061 atgggtctgg gtaccgatat cgacactggc ctgccgatga gcctgtccat gaacgtctat
    21121 gcgaaatacc agtggcagaa ctatggcgca gcgaacgaaa acgagtggga cggttaccgt
    21181 ttcaaaatta aatactttgt gccgattacc gatctgtggg gcggtcagct gagctacatc
    21241 ggcttcacca acttcgactg gggttccgat ttaggggatg acagcggtaa cgcaatcaac
    21301 ggtattaaga cccgtactaa taactctatc gcttccagcc atattctggc tctgaactac
    21361 gatcactggc actactctgt cgtagctcgt tactggcacg acggtggtca gtggaacgac
    21421 gatgcagaac tgaacttcgg caacggcaac ttcaacgttc gctctaccgg ctggggtggt
    21481 tacctggtag taggttacaa cttctgatta tgaaaatgcc gggatttatt cccggcattt
    21541 ctgattgtta acaacatgtg ctttgttcat gccggatgcg gcgtaaacgc tttgtccggc
    21601 ccacaaaagc acgtaaattc aatatattgc tgaaataacg taggcctgat aagcgtagcg
    21661 catcaggcaa ttttacgttt taatcacttc ttcttgttca tcatcgcttt cagatcagcg
    21721 aatgggttgt acttcgcttc accgacatct ttctgcgcat cttcccctgc gataacggtc
    21781 gtaccatact gatccgcttc ggtatatttc gaatgctcat gatcgtggca atagagacac
    21841 aacaattccc agttactgcc atcttccggg ttattggtat ggtcgtgatc aatgtggtga
    21901 acggtaagtt cacgcaggtt agaataaaca aactcgcggg aacagcgacc gcagacccac
    21961 ggatagattt ttaatgcttt ttcgcgatag ccgctttcta accgtgcata gttttttggg
    22021 atgatagcca tgaaaagtta cctggatgaa atgtagggtt acgcgcagtt tcccaagatg
    22081 cggggatttt cgcaatctcc atacatcaaa aaaaatcccg atcttctgac cgggattttc
    22141 aacatcaacg ggaacttgat taaggcagaa ttgacggctg atccgcccct tctttttcca
    22201 ctttctgctg caacatgtgt tcgcgcttca tacccagttt cagagccaac gcagatgcta
    22261 cgtagatgga agatgcagtg ccgatggaaa caccgataag catggtcagc gagaagcctt
    22321 ccagtaccgg accaccgaag agatacagca tcaggataac catcaaggta gtaccggatg
    22381 tgatcaaggt acggtgcagc gtctgggtca aggacacgtt aaagatttcg taaggcgtac
    22441 cgcgacggat cttgcggaag ttttcacgaa ttcggtccga taccacgata ctgtcgttaa
    22501 gcgagtaacc gataaccgac atcaacgatg ccacaatggt caggtcaatc tcgatatgga
    22561 ataacgacaa aatacccagc gtaatgataa cgtcgtgcgc cagcgcaata accacccctg
    22621 ccgccagtcg ccactcaaag cggaaaccta cgtacacgag gatagacagc agcgctgcca
    22681 tcaacgccat cgcaccggtt tgcgcaaggt ctgcccccac gctcggaccg acgaactcaa
    22741 tacgcttcac tgctgcattc tgattggtgg attcgttaat caccttcaga acttggctgc
    22801 ccagcacctg accgccggtt tcgccttcag caggcggcat acggaccatg atgtcgtggc
    22861 tgctaccaaa gttttgcagc attggctctt caaaaccggc tttttgcaat gcatcacgca
    22921 ttacgtcaat ttcagccggt ttttcgagcg taatttcgat aaccgtacca ccggtgaaat
    22981 ccagccccca gttaaagccg cgcacgccca taataacgat agcagcgatt aacagcagac
    23041 cagagatgcc gaaagcccag tagtcccagc gcataaagtc atagacttta cggccgtggt
    23101 ttagttgttc aacagtatat tcctgtgcca catcgcactc ctcagattga cagcttcttg
    23161 acgcgcttgc cgccatatag caggtttacg atggcacgcg taccgacaat cgcggtaaac
    23221 atcgacgtcg ccacaccgat accggtagta atcgcgaacc ctttaattgc cccggtaccc
    23281 actgcgtaca ggatgatgac tttaatcagc gtggtgatgt tcgcatcgaa gatagaactg
    23341 aatgcgccac gataaccttc atcaattgcc tgttgaacag tacgtccgtt gctcaactct
    23401 tctttaatac gttcgttgat cagtacgttc gcatcgaccg ctaccgcaag ggttaagacg
    23461 atacccgcga tacctggcat actcagtgtt gcgcctggca acagcgacat aataccgacg
    23521 atcaagatca agttggcaat cagagcactg gtcgcaatca gaccaaactt cttatagaag
    23581 atgatcatga acagaataga aaccagcaga ccggcaaggc aagcctccag cccctgctca
    23641 atgttctgca tacccagggt cgggccaatt gtacgttctt caacaatctg aatcggcgcg
    23701 atcaacgcac cggcacgcag cagcagtgac agctgacggg cttcgttcgg gttgttgatg
    23761 ccggtgatac ggaagctgtt acccagacga gactggatgt tggcgatgtt aatcacctct
    23821 tcctgtttca ccagaaccgc acgaccattt gcatctttct taccgctgtc tttgtactcc
    23881 acaaacaggg ttgccatcgg tttgccgatg ttgtccttgg tgaagttaga cataatgttg
    23941 ccaccagcgc tatcgagcga gatgttaacc tgcggctggt tgtattcgtc ctggctggaa
    24001 gtggagtcgg tgatatggtc accagtcaga attacgcgtt tgtacagcac aaccggctga
    24061 ccttcgcggg tctgtttcac ttcagagtcg cccggtacgc gaccggatgc cgcagcggcc
    24121 tgatcaacat tggtgtttac cagacggaat tccagcgttg ccgtcgcgcc cagaatctct
    24181 ttcgcacgcg cagtgtcctg aataccaggc agttcaacaa cgatacggtc agcaccctga
    24241 cgctgaacca ccggctccgc cacgccaagt tggtttacac ggttacgcag gatattaatg
    24301 ttctgctgca ccgcatattc acgcgcttca ctcagacgag catcgctcat taccgcacgc
    24361 agctggttgc tgccctggct gctaatcacc aggtccggat ggcgcttgct cagatacgca
    24421 atggcttcat cacgagcttt agcatcgcgg aaagtgatgc tcaggccgta gttgttttct
    24481 ttacgaacag tggtatacgg gatgcccttt tcgcgcaggt cactgcgtag gctatcgata
    24541 ttttgttcct ggagtttgcc aagcgcggtg tccatatcca cttccatcag gaagtgaacg
    24601 ccgccacgca ggtcaaggcc gagcttcatc ggctcagcat gaatagctgc cagccagcgc
    24661 ggcgttgccg gggcaaggtt aagcgccacg acgtatttgt cacccataac gcccattaat
    24721 gcttcacgag cgcgcaactg ggtgtcagtg gagtcgaagc gcgcaagaat agcgccctct
    24781 tccagtgcca cagacttagc agttattttt tcttcttgta acgttttctg gacctggatc
    24841 agcgtttgct cactggcggc gactccgcgc gcaccagtga tctgaacagc cggatcctca
    24901 ccaaacaggt tgggaagcgc atacagcaga ccgatgacaa tcaccacgat cagcatgacg
    24961 tacttccaca aaggataacg gtttaacacg gcaattccct tagggaaaaa ttttaattac
    25021 agcgccttca tggtgccttt cggcaggacg gcagctacga agtcacgttt aataactact
    25081 tcagtggtgt cattcagcgc gatagcaatg tagccgtttt ccgctacttt ggttacgcga
    25141 ccaaccaggc caccgttcgt cagaacttca tcacctttgg caatggagtc catcagcttt
    25201 ttgtgttctt tggtgcgctt ctgctgtgga cgcaggatca tgaaatagaa aatcagaccg
    25261 aataccacca gcatcaaaat caaagacatc gggctacctt gcgccggtgc acccgttgcc
    25321 gctaccgcat cagaaataaa aaagctcatt aaatttccct cattattaat attaatcaac
    25381 gttcaaaggt ggtacttctc gcccctgacg ctggtaaaaa tcagttacga agctctctaa
    25441 tttaccctct tcaatagcct tgcgtaaacc cgccatcaaa cgctggtagt aacgaaggtt
    25501 atgaatggtg ttgagtcgcg cgcctaatat ttcgttgcaa cggtcgagat gatgcaagta
    25561 agcgcgtgaa taattgcgac aggtgtagca atcacactca ggatcgagtg ggccagtatc
    25621 gctcttatat ttcgcattgc ggattttcac cacgccatcg gtcacgaaca aatgaccatt
    25681 tcgggcgttg cgggttggca ttacgcagtc aaacatatcg ataccgcgac gtacgccttc
    25741 aaccaggtct tccggtttac caacgcccat caggtaacgc ggtttgtctg ccggaatttg
    25801 cggacacaca tgctccagaa tacggtgcat atctgctttc ggctcaccca cagccagacc
    25861 gccgacagcg tagccatcaa agccgatatc taccagacct ttaacagata tatcacgtaa
    25921 atcttcgtaa atactgccct gaattatacc aaataacgca tttttgttac caagactgtc
    25981 aaaacgctca cggctacgct tcgcccaacg cagagacatc tccatagagc gttttgcgta
    26041 atcccagtca gcaggatacg gcgtacactc atcaaagatc atgacgatat ccgaaccaag
    26101 atcgtactga atctccatcg acttttccgg gtcgaggaaa atcgggtcgc cgttgatcgg
    26161 gttacggaag tgaacgccct gttcggtgat tttacgaata tcaccaaggc tgaagacctg
    26221 gaagccgccg gagtcggtaa gaatcggtcc tttccactgc ataaaatcgt gcatatcgcc
    26281 gtgcagtttc atgatttcct ggcccgggcg cagccacagg tggaaagtgt tgccgaggat
    26341 aatttgcgcg ccagtggctt caacttcttc cggcgtcatc ccttttacgg tgccgtaggt
    26401 gccaacaggc ataaaacaag gcgtttccac tacgccacga tcaaagacca ggcggccacg
    26461 gcgtgcgcga ccgtcggtgg tgtccagttc aaatttcatt ttttctccac tacgtcagaa
    26521 aaacagtcca acgtttaaac cagcgccgcg gaattactcc ccgacgcgct cattaattgc
    26581 ctgcggattg tacgtgataa acatcgcatc accgtaacta aaaaagcgat atttctcttc
    26641 taccgccgct ttataggcat tcatggtgtg ttgataaccg gcaaaggccg aaaccagcat
    26701 aatcagcgtc gactctggca agtggaagtt cgtcaccagc gcatcgacca ctttgtactg
    26761 gaagcccgga tagataaaga tttgggtatc gtcaaagaac ggttcgataa gatcgttttt
    26821 tgccgcctgt gccgcgcttt ccagcgaacg tactgaagtg gtgccaaccg caatcacccg
    26881 gttaccgcgc gctttcgccg ccagtaccgc gtctaccaca tcctgcggta cttcagcgta
    26941 ttccgagtgc atgatgtgat cttcaatggt gtcgacgcgc accggctgga aggtgcccgc
    27001 accaacgtgc aacgtcacaa acgccatctc cacgcctttg gcgcgtaatt tttccagcaa
    27061 aggctcgtca aaatgcagac cagcggtcgg ggctgcaacc gcgcccggtt tttcgctata
    27121 aacggtttga taaagttcgc ggtcagcgtc ttcgtccgga cgatcgatat acggcggcag
    27181 cggcatatgg ccgatgctgt tgaggatatc cagtaccgag cgttcatcat taaattcgac
    27241 ttcaaacagt gcgccgtggc gcgcggtcat tgttgcgttg atactttcgt catcgcccag
    27301 caacagttct gcgccaggtt ttggcgcttt cgaggcgcga atatgcgcaa gaatgcgttt
    27361 gtcgtcgagc atccgctcaa ccagcacttc aatcttaccg ccgctggctt tacgcccaaa
    27421 cagacgcgcc gggatcacgc gggtattatt aaaaaccaga agatcgccgg ggttgagctt
    27481 atcgagtaaa tcggtgaaag taccgtgcgt cagcgcaccc gtcggcccgt ccagcgacag
    27541 taaacgacag ctactgcgtt caggcatggg atagtgggca atcagggatt cgggcaattc
    27601 aaaggagaaa tcggtaacgc gcatgacact gactcagact aaaataagag tcgggtagtc
    27661 tagtgccggg gcgctctccc tgcaaccttt accccccttt ggatagaata taatcgatga
    27721 attttttagc tcacctgcat ttagcccatc tcgcggaaag ctcgctttct ggcaatttac
    27781 tggctgattt cgtacgcgga aatcctgaag aaagttttcc gcccgacgtc gtggctggca
    27841 ttcatatgca tcgacgtatt gactgacaat ctgccggaag tccgcgaagc acgggagtgg
    27901 tttcgtagtg aaacgcgccg cgttgcgcct attacgctgg atgtcatgtg ggatcacttt
    27961 ctttcccgcc actggtcgca gctgtcgccg gactttccac tacaggaatt tgtctgttat
    28021 gcccgcgagc aagtgatgac gattttgccg gactcaccgc cacgttttat caatctgaac
    28081 aattacttgt ggtcagagca gtggctggtg cgctatcgcg atatggattt catccagaac
    28141 gtgttaaacg gcatggcaag ccgccgccca cgtctggatg ccctgcgtga ctcctggtac
    28201 gatttagacg ctcattatga cgccctcgaa acccgcttct ggcagtttta tccgcggatg
    28261 atggcgcagg cgtcacacaa ggcgttataa aacgttagtt catccatacc gtagccgaaa
    28321 tggcaggcaa agccagaatc ccgtcagtca gttgcccatg cccttctttg cattgccatt
    28381 gcacggcatt gagaaacggt gacgcgggta gcaccacttc actggcctcg ccacggttga
    28441 ttgccaccag tacacgttgc tgattcagca cgcggacaaa taccactacg ttatcttccg
    28501 catacagcac ctgacagccg ccacgacgca gcgcctgact tttcttacgc agcacaatca
    28561 ttcgccggta cagcgcgaat aacgccgtat cctgcttttc cacctgccag gggaacggtt
    28621 tacggcaaga cggatcgttt ttaccatcca gtcccacttc atcaccgtaa taaatgcacg
    28681 gtacaccagg ccaggtgaac agccagacca ccgccagcgg caggcgcgca atatcccgac
    28741 cgagcagcgt tttaaatcgc gcagtatcgt ggctatcgag ctgattaaac atacgtaatt
    28801 gttgttgatg agaaagccct gcgcggtaat tatccatcca ggccatacag gtttgggcgt
    28861 caatttgctg cggatcataa gagatgtcgg tattggcaag aaatccccac aacgggaatg
    28921 tgaagccacg atagttcatg gcagcatctt ccacatcggc ctgtaaccat tgccgtgcat
    28981 cgccaaaatg ttcgccgaca atatacgctt ccgactgggt ttctttcgcc gcttcggtga
    29041 tcccggcaac gtgctgcata ttattgcgcg ccccacccgc ctcccccagc atatgcacca
    29101 catccagccg ccagccgtcc atattccacg gcgctttcag ccagtggcgg acaatactgt
    29161 cttccccgcg ataaatttca ttcaccagac tttccgactg ataatccagc ttcggcaagc
    29221 tggcatagcc aagccagtcg agcgccgtgc catcatcact aaacaagtac cagtcgcgcc
    29281 agggcgattc agagttgtga caagctcccc ccgtgccaag attgtgcctg tca
//
LOCUS       DADMGC010000040        28273 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.040, whole genome shotgun sequence.
ACCESSION   DADMGC010000040 DADMGC010000000
VERSION     DADMGC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28273
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.040"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..1101
                     /gene="pdeC"
                     /locus_tag="JNZ73_001735"
     CDS             <1..1101
                     /gene="pdeC"
                     /locus_tag="JNZ73_001735"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HAY8732042.1"
                     /translation="SEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLT
                     VQRNGYLYATIYSTKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRI
                     RQNYLSPKRKLQRALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQIMNPAEFI
                     PLAEKEGMIEQITDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKT
                     EQYAVRPQQIKFEVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLN
                     VDILKIDKSFVETLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVR
                     YCQGWFFAKAMPPQVFMQWMEQLPARELTRGQ"
     gene            complement(1104..1427)
                     /gene="soxS"
                     /locus_tag="JNZ73_001736"
     CDS             complement(1104..1427)
                     /gene="soxS"
                     /locus_tag="JNZ73_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HAY8732043.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1513..1977
                     /gene="soxR"
                     /locus_tag="JNZ73_001737"
     CDS             1513..1977
                     /gene="soxR"
                     /locus_tag="JNZ73_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HAY8732044.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2681..4030
                     /locus_tag="JNZ73_001738"
     CDS             2681..4030
                     /locus_tag="JNZ73_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY8732045.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4181..5830
                     /locus_tag="JNZ73_001739"
     CDS             4181..5830
                     /locus_tag="JNZ73_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HAY8732046.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(5984..7276)
                     /locus_tag="JNZ73_001740"
     CDS             complement(5984..7276)
                     /locus_tag="JNZ73_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY8732047.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKENDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(7454..9103)
                     /gene="actP"
                     /locus_tag="JNZ73_001741"
     CDS             complement(7454..9103)
                     /gene="actP"
                     /locus_tag="JNZ73_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HAY8732048.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLITERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(9100..9414)
                     /locus_tag="JNZ73_001742"
     CDS             complement(9100..9414)
                     /locus_tag="JNZ73_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HAY8732049.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(9614..11572)
                     /gene="acs"
                     /locus_tag="JNZ73_001743"
     CDS             complement(9614..11572)
                     /gene="acs"
                     /locus_tag="JNZ73_001743"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HAY8732050.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPKAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            11965..13401
                     /gene="nrfA"
                     /locus_tag="JNZ73_001744"
     CDS             11965..13401
                     /gene="nrfA"
                     /locus_tag="JNZ73_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HAY8732051.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALEEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDKQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            13446..14012
                     /gene="nrfB"
                     /locus_tag="JNZ73_001745"
     CDS             13446..14012
                     /gene="nrfB"
                     /locus_tag="JNZ73_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HAY8732052.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            14009..14680
                     /gene="nrfC"
                     /locus_tag="JNZ73_001746"
     CDS             14009..14680
                     /gene="nrfC"
                     /locus_tag="JNZ73_001746"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HAY8732053.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCEHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            14677..15633
                     /gene="nrfD"
                     /locus_tag="JNZ73_001747"
     CDS             14677..15633
                     /gene="nrfD"
                     /locus_tag="JNZ73_001747"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HAY8732054.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEYDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            15653..17371
                     /locus_tag="JNZ73_001748"
     CDS             15653..17371
                     /locus_tag="JNZ73_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY8732055.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSMLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            17364..17747
                     /gene="nrfF"
                     /locus_tag="JNZ73_001749"
     CDS             17364..17747
                     /gene="nrfF"
                     /locus_tag="JNZ73_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HAY8732056.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            17744..18340
                     /gene="nrfG"
                     /locus_tag="JNZ73_001750"
     CDS             17744..18340
                     /gene="nrfG"
                     /locus_tag="JNZ73_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HAY8732057.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            18682..19995
                     /gene="gltP"
                     /locus_tag="JNZ73_001751"
     CDS             18682..19995
                     /gene="gltP"
                     /locus_tag="JNZ73_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HAY8732058.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWSLIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(20073..20762)
                     /locus_tag="JNZ73_001752"
     CDS             complement(20073..20762)
                     /locus_tag="JNZ73_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY8732059.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDQRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(20856..23003)
                     /gene="fdhF"
                     /locus_tag="JNZ73_001753"
                     /pseudo
     CDS             complement(20856..23003)
                     /gene="fdhF"
                     /locus_tag="JNZ73_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(23201..24667)
                     /gene="mdtP"
                     /locus_tag="JNZ73_001754"
     CDS             complement(23201..24667)
                     /gene="mdtP"
                     /locus_tag="JNZ73_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HAY8732060.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(24664..26715)
                     /gene="mdtO"
                     /locus_tag="JNZ73_001755"
                     /pseudo
     CDS             complement(24664..26715)
                     /gene="mdtO"
                     /locus_tag="JNZ73_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            complement(26715..27653)
                     /locus_tag="JNZ73_001756"
     CDS             complement(26715..27653)
                     /locus_tag="JNZ73_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HAY8732061.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADIALTKLHLEMTTVRAPFDGRIISLKTSVGQFASAMRPIFTLIDTRHWYVIA
                     NFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGLPKVSRSI
                     NWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEPQ"
     gene            complement(27672..27947)
                     /locus_tag="JNZ73_001757"
     CDS             complement(27672..27947)
                     /locus_tag="JNZ73_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732062.1"
                     /translation="MPTVLSRMAIHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(28156..>28273)
                     /locus_tag="JNZ73_001758"
     CDS             complement(28156..>28273)
                     /locus_tag="JNZ73_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732063.1"
                     /translation="ADLVKAKRAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         6450 a         7156 c         7596 g         7071 t
ORIGIN      
        1 agtgaagtga tgtctgatga cccgacattg caatggggtg tgtatgatac ggtgacgaaa
       61 acctttttct cgttaagcaa agaggcctcg gcagcaacgt tttctccgct gattcatttg
      121 aaggatttaa ccgtacaaag aaatggctat ttatatgcga caatttattc gacaaaacgc
      181 ccaattgcag ccattgttgc gacttcatat caacgtctta taacccattt ttataatcat
      241 cttatttttg cgttgcccgc cggtattttg gggagtcttg ttctgctatt actctggcta
      301 cgtattcgac aaaactattt atctcccaaa cgtaaattgc aacgcgccct cgaaaaacat
      361 caactttgtc tttattacca gccaataatc gatatcaaaa cagaaaaatg tatcggcgct
      421 gaagcgttgt tacgttggcc tggtgagcag gggcaaataa tgaatccggc agagtttatt
      481 ccgctggcag aaaaggaggg gatgatagaa cagataactg attatgttat tgataatgtc
      541 ttccgcgatc tgggcgcgta cctggcaaca catgccgatc gctatgtttc tattaacctg
      601 tcggcctccg attttcatac gtcacggttg atagcgcgaa tcaatcagaa aacagagcaa
      661 tacgcggtgc gtccgcagca aattaaattt gaagtgactg agcatgcatt tcttgatgtc
      721 gaaaaaatga cgccgattat tctggctttc cgccaggctg gttacgaagt ggcaattgat
      781 gattttggta ttggctactc taacttgcat aaccttaaat cattgaatgt cgatattttg
      841 aaaatcgata aatcgtttgt tgaaacgctg accacccaca aaaccagtca tttgattgcg
      901 gaacacatca tcgagctggc gcacagcctg gggttaaaaa cgatcgctga aggcgtcgaa
      961 actgaggagc aggttaactg gctgcgcaaa cgcggcgtgc gctattgcca gggatggttt
     1021 tttgcgaagg cgatgccgcc gcaggtgttt atgcaatgga tggagcaatt acccgcgcgg
     1081 gagttaacgc gcgggcaata aaactacagg cggtggcgat aatcgctggg agtgcgatca
     1141 aactgccgac ggaaaacgcg ggagaaggtc tgctgcgaga cataacccag gtccattgcg
     1201 atatcaaaaa tcggacgctc ggtggtgcgc aactcaacgg cggccagtaa caggcggcgt
     1261 tggcgaatgt aatcgccaag cgtctgatgc gtcaccgtgc ggaacatccg ttgcaagtac
     1321 cactttgaat agcctgattt ttttgcgacg acatcaatgt taagcggctg gtcaatatgc
     1381 acgtcaatcc atgcgataag atcctgaata attttctgat gggacataaa tctgcctctt
     1441 ttcagtgttc agttcgttaa ttcatctgtt ggggagtata attcctcaag ttaacttgag
     1501 gtaaagcgat ttatggaaaa gaaattaccc cgcattaaag cgctgctaac ccctggcgaa
     1561 gtggcgaaac gcagcggtgt ggcggtatcg gcgctgcatt tctatgaaag caaagggttg
     1621 attactagta tccgtaacag cggcaatcag cggcgatata aacgtgatgt gttgcgatat
     1681 gtcgcaatta tcaaaatcgc tcagcgtatt ggcattccgc tggcgaccat tggtgaagcg
     1741 tttggcgtgt tgccagaagg gcatacgtta agtgcgaaag agtggaaaca gctttcgtcc
     1801 caatggcgag aagagttgga tcggcgcatt cataccttag tggcgctgcg tgacgaactg
     1861 gacggatgta ttggttgtgg ctgcctttcg cgcagtgatt gcccgttgcg taacccgggc
     1921 gaccgcttag gagaagaagg taccggcgca cgcttgctgg aagatgaaca aaactaaagc
     1981 gccacaagga caagctgact catcgttaac tatgaggccg gggtgagggt aaatatttgc
     2041 actggtgctg cctgttcccc tcaccctaac cctctccccg aaggggcgag gggactgatc
     2101 gagctctttt ggaattatcc gcaaacacaa aactaaagcg ccacaagggc gctttagttt
     2161 gttttccggt ctttgtcttt ctctctatcc cgctggaaca caggagggtt tcccccgacg
     2221 tcaacacacc tcattcgagc acgtggtgga ggttccggtt ggtgttgatg ctttaattgt
     2281 atgtcaccga cgtttcttcg ccagtgtaaa agtatacttt ttagccgcaa tatttttgtc
     2341 atctcagacg attttttatc gcaatccaga acggtatacg gctcgataac gctgcaatct
     2401 tgcacatcga cgataacgtt tgcgcatcaa ttgcctggtt tttcatcgtc aagacaataa
     2461 aagagaaaaa agcagcaaac ttcggttgaa aaagccgcta tgatcgccgg ataatcgttt
     2521 gcttttttac cacccgtttt gtatgcgcgg agctaaacgt ttgctttttt gcgacgcacc
     2581 aaattgccgc aaacctggag caggaagata acgtttcgct ggcaggggat tgtccgccac
     2641 gcatcttgac gaaaattaaa ctctcagggg atgttttctt atgtctacgc catcagcgcg
     2701 taccggcggt tcactcgacg cctggtttaa aatttcacaa cgtggaagca ctgtccgtca
     2761 ggaagtggtt gccgggttaa caacgtttct ggcgatggtc tactcggtca tcgtcgttcc
     2821 aggtatgttg ggtaaagcag gcttcccgcc tgcggcagtt ttcgttgcaa cctgtctggt
     2881 tgccggactc ggttctatcg tgatgggtct gtgggctaat ctgccgttgg cgattggttg
     2941 cgccatctcc ctgacggcgt ttaccgcatt cagcctggtg ctggggcaac atattagcgt
     3001 acctgtcgcg ctgggtgccg tgttcctgat gggggtgctg tttacggtaa tttctgccac
     3061 gggtatccgt agctggattt tgcgcaactt gccgcacggt gtggcgcacg gcacggggat
     3121 cggtatcggt ctgttcctgc tgctcattgc cgctaatggt gtcggtctgg tgattaaaaa
     3181 cccgcttgat ggtctgcccg ttgcgctggg tgatttcgcg accttcccgg tgattatgtc
     3241 actggtaggt ctggcggtga tcatcggctt ggaaaaactg aaagtccctg gtggcattct
     3301 gctgaccatt atcggtatct caattgtcgg tttgatcttc gatcctaacg tccatttctc
     3361 cggcgttttc gccatgcctt cattgagcga tgaaaacggc aattcactga ttggcagcct
     3421 ggacattatg ggcgcgctga atcctgtagt cctgccaagc gttctggcgc tggtgatgac
     3481 ggcagtattt gatgccaccg gaactatccg cgctgtcgcc ggccaggcga acctgctgga
     3541 taaagatggg cagatcatcg acggcgggaa agcactgacc actgactcca tgagcagcgt
     3601 tttctctggc ctggtgggtg cagctccggc agcggtatac atcgagtctg cggcgggtac
     3661 ggcggcgggc ggtaaaaccg ggttgacggc tatcaccgtt ggcgtgctgt tcctgctgat
     3721 tctgttcctc tctccgcttt cttacctcgt tccggggtat gcaacggctc cggcgctgat
     3781 gtacgttggc ctgctgatgc tgagcaacgt ggcgaaaatc gactttgctg attttgttga
     3841 tgcgatggcg ggtctggtta cggcggtatt catcgtgctg acctgtaaca tcgtaacagg
     3901 catcatgatc ggcttcgcga ctctggtgat tggtcgtctg gtttccggtg aatggcgcaa
     3961 gttgaacatc ggtacggtcg ttatcgccgt ggcgctggtg accttctatg cggggggctg
     4021 ggctatctaa tctttcatcg gattctgaaa acgggtggca atggctgccc gtttttattt
     4081 tctccgcaca ttgtgtgagc ttttgcgata ttctgaaaaa atgagaattc aggcataacg
     4141 tctttctcag atcataaaaa acatcgcaaa cagggaacgc atggaaatct tcttcaccat
     4201 actgataatg accctcgtgg tctcgctgtc cggggtggtc actcgtgtca tgccctttca
     4261 gatcccgctt ccgcttatgc aaatcgccat cggtgcgcta ctggcgtggc cgacgtttgg
     4321 tttgcatgtg gagtttgatc ctgaactctt tttagtcttg tttatcccgc cgttgctgtt
     4381 cgctgatggc tggaaaacgc cgacccgtga atttctcgaa catggtcgag agattttcgg
     4441 cctcgcgctg gcgctggtgg tggtcaccgt ggtcggcatt ggcttcctta tttactgggt
     4501 ggtgccgggc attccgctga tccccgcctt tgcgctggcg gcggtacttt ctccgaccga
     4561 tgctgtggcg ctctccggga ttgttggcga agggcgcatc ccgaaaaaaa tcatgggcat
     4621 tttgcagggc gaagcgttga tgaatgacgc ttccggcctg gtgtcgttga agtttgccgt
     4681 ggcagtggcg atggggacga tgatcttcac tgtcggcggt gcgacggtcg aatttatgaa
     4741 agtagccatt ggcggtattc ttgccggttt tgtggtgagc tggctgtatg gtcgctcgct
     4801 gcgattcctc agccgctggg gcggtgatga acccgcgacg cagatcgttc tgctgttctt
     4861 gctgccattc gcttcttatc tgattgccga acatattggc gtttcgggca tcctcgctgc
     4921 ggttgccgcc gggatgacca tcacccgctc cggtgtgatg cgccgtgcgc cactggcaat
     4981 gcgcctgcgc gcaaacagca cctgggcgat gctggaattt gtctttaacg gcatggtgtt
     5041 cctgctgtta ggtctgcaac tgccgggtat tctggagacg tcgctgatgg cggcagaaat
     5101 cgaccctaac gtcgaaatct ggatgctgtt taccgatatt attctgatat atgcggcgct
     5161 gatgctggtc cgtttcggct ggctgtggac gatgaaaaag ttcagcaacc gcttcctgaa
     5221 gaagaagccg atggagtttg gttcgtggac cacacgagaa atcctgatcg cgtctttcgc
     5281 tggggtgcgt ggggcgatca ctctggccgg tgtgctctca atcccgctgc tcttgccgga
     5341 tggtaacgtc ttcccggcgc gctatgagct ggtgttcctg gcggctggcg tcattctctt
     5401 ctcgctgttt gtcggcgtgg tgatgttgcc tattctgcta caacacattg aagtcgcgga
     5461 ccattcgcaa caattgaaag aagaacgtat tgcgcgagcg gcaacggcag aagtggcgat
     5521 tgtggcgatc cagaaaatgg aggagcgtct ggcggcggat accgaagaga atatcgataa
     5581 ccagttgctt acggaggtca gttctcgcgt cattggtaac ctgcgtcgtc gcgccgatgg
     5641 acgtaacgac gttgaaagtt ccgtgcagga agagaacctt gagcgtcgct tccgtctggc
     5701 ggcattgcgt tctgaacgtg ctgaactgta ccacctgcgc gccacgcggg agatcagcaa
     5761 cgaaacgctg caaaaattac tgcacgatct cgatttgctt gaagcgttgc taattgagga
     5821 aaatcagtaa gccgaaagcg acgtaaccag gcccgaggta atctgaaaaa ggtgcaatct
     5881 tttgcacctt taacaggtta gctcgggcga acattcatga ttctaaaaca aataagttat
     5941 ctctcttgta aagtcaattc attggctgtt cttcgactta acctcaatct gcgtgttgca
     6001 taatatctgc agtaatcttc atagcgactg cattaatatt atcctcagtt tgtgacatgc
     6061 cagtatgaga ggtacaatct tcttcaaaga atttctgtag tgttaccagg gtatcactat
     6121 caatggggat attttcctcc ccacggagaa tggaaatgcc cattccctgg acgctagcct
     6181 catcgaaatt aacccgatag agacgggtat taaacataca ggcttttgtt aaaatagaac
     6241 agttgaaatg gcagttgctt aaattggcat tattaaaatt aacattagcc atattagagc
     6301 cggtacatat aaccatttcg aggaatgtat ttgtcagcca cgtacaaaag agtttgctat
     6361 caattaagtt tatattctgg aggattgctt tgttcagatt gacgcgagac agatcagcat
     6421 gttcaaagtt aattaaatcg agtttagaga aactaaggtc ggctttaaat aaattaacat
     6481 atggcgctat tactttataa agatttacct cagccatgta cgccatataa aaatttgaat
     6541 agtcaaggaa ggtattttta atattggctc tattcattgc tgcattgtag aattgaacat
     6601 gcatagcgga gcatgcattt agagttccgt cagccatttt tatctgagcg aaatttgagt
     6661 catcgagaat acaattatca aaaagtattt tttccattat ggcgtaataa aaattcgttt
     6721 tatacatatt acagtcctgg aaaatagcgt tttgtagtct gcaacctgca aaattcgaat
     6781 tggataaatc ttcatcttta atcacgatac ccttcaggct aaggtgtgaa aaatcgtaac
     6841 caccatttac tatctgtgga atttcgatag tattattatg gacggcgaca ttatttataa
     6901 aatataccac cttaatggat acatcatttt ctttactaag gtaaactctt ctcgtaattc
     6961 tatcgtcaca tttattattt gcatataacg ctatatctat aaaatattta tgtattttcc
     7021 tgtccgtttc tttcgtgatt tttcctgatg gtaaactatc ataaattctt ttcattttct
     7081 gaatcaagcc tatcactttt tcatgatcat ctattttgaa aagagagagg gctttattgt
     7141 caattaacgt gtttttatga tgtttgctgt aattatctga ttttgttttc tttgtatgtg
     7201 atgaacttgt gatagagtgg ttatcgttaa tctggcaaag agggaaaaac atattattta
     7261 aaccattata gcgcataaaa tatccttata attaacaaga aagaaaaggc atattctctg
     7321 cattatcatt ttctacgact gtcaaaaatc gctcattttt taatgagttt atttgtttaa
     7381 tattatggga aaaggtgatg catgttggag aggaagagta ttccccggtc aggcgaccgg
     7441 ggaaggggag agattaatgt gcgcggcctt gctcaacgcc aaagccggtc tgggagcgga
     7501 taaactgcgc gcggaacagt tcacgctcgc gcgccccttc cgctgagtta tcggttgccg
     7561 agaagaacca gatgccgagg aatgccacgc tgatagagaa cagcgccggg tattcatacg
     7621 ggaagatggc tttttcgtga ccgaggatct gtacccaaat agtcgggccg aggatcatca
     7681 gcaccactgc ggtaatcagc cccagccagc cgcccagcat cgcgccacgg gtagtcagtt
     7741 tcgaccagta catggaaagc agaatgatcg ggaagttaca gctcgccgcg atggcaaacg
     7801 caaggcctac cataaaggcg atgttctgat tctcaaacag cacgccgagg ataatcgcaa
     7861 tcacgccgag gatcagtacg gtgatttttg atacccgcag ctcttcacgt tcggtcgcgc
     7921 cttttttgaa gacgttagcg tacaagtcat gcgaaaccgc cgatgcgccc gccagcgtca
     7981 gacccgcaac caccgcgagg atagtggcga aagcaaccgc tgaaataaaa ccgaggaata
     8041 ggttgccgcc cactgcattc gccaggtgaa cggccgccat gttgttacca ccaatcagat
     8101 ggcccgccgc gtctttatat tccggattcg caccaacaag catgatcgcg ccgaagccga
     8161 taataaaggt cagaatatag aagtagccca taaatccggt ggcgtagaac acgctcttac
     8221 gtgcttcgcg ggcatcgctg actgtaaaga agcgcatcag aatgtgcggc aagcccgccg
     8281 taccaaacat cagtcccagc cccagagaga gcgcggagat cggatctttc accagtccgc
     8341 ccggtttcat gatgtcgaca cctttcgggt gtaccgccat cgcttcgctg aacagattgt
     8401 tgaagctaaa gccgacgtgt ttcatcacca taaaggccat aaagctggca ccgaacagca
     8461 acagcacagc tttgataatt tgtacccagg tggtcgccag catgccgccg aacaggacgt
     8521 acatcatcat cagcacgccg accagcacca ccgcaatgtg atagttaagg ccaaacagca
     8581 gctcgatcag tttacctgcg cctaccatct gagcgataag gtaaagcgcc accaccacca
     8641 gagaaccaca ggccgaaaga atacgaatcg gcccttgttt cagacgataa gaggccacat
     8701 cggcagaggt gtagcgtccc aggttacgca gacgttcggt aatcagaaac agaatgattg
     8761 gccagcccac caggaagccc aacgagtaga tcagcccgtc atagccggag gtaaacacca
     8821 gcgcggagat ccccaagaat gaggcggcgg acatatagtc cccggcaatc gccagcccgt
     8881 tctggaagcc agtgatattg ccgcctgcgg tgtagtagtc gctacgagaa cgtacgcgtt
     8941 ttgatgccca gtaggtaatg ccgagcgtaa acacgacgaa aatcaggaac ataataatcg
     9001 cctgccagtt cgttggctgg cgctctacgg ccccgctaat agcatccgcg gcgttagctg
     9061 cgaaagggag tgtggcggca agcgccgtca gaactctttt catgatgctt gtacctcatg
     9121 caggacttca ttattaagac ggtcgaattc gccgttcgcc cgccagatgt agataccggt
     9181 gagaacaaag gagatcacaa tcactccaac accaattgga ataccgcgtg tgacgctggt
     9241 gttcggattc agcggcgtgc ccagccagcc gggcgcgaag gcgatcagta aaataaagcc
     9301 gatataaact gccagcataa taatcgacag gatggtggca aaccgttgcc gtttttcgac
     9361 taactccctg aaatgcgcat tgtcttctat ccgctgataa atcgtaccat tcatcacaga
     9421 ttctccagag gtaatgtagg gattgtttta attcccgctc ccttatggga gaaggttaac
     9481 gctcgggtaa cccttgccga atgtaggccg gataaggcgt ttacgccgca tccggcaatc
     9541 aatgcctgat gcgacgctgt cgcgtcttat caggcctaca aaccgttacc gattcgcatc
     9601 cggcaattgt gggttacgat ggcatcgcga tagcctgctt ctcttcaagc agcttctcga
     9661 ccacgccagg atcggcaagc gtcgaggtat cgcccaggtt gctggtatcg cccgccgcaa
     9721 ttttgcgcag aatacggcgc ataattttgc cggagcgggt tttaggcagg gagtcggtcc
     9781 agtgcagcac gtctggcgtc gccagcgggc caatctcttt acgcacccag ttgcggactt
     9841 ctgcgtacag ttctggtgac ggttcctccc cgtgattaag cgtgacatag gcgtagatcg
     9901 cctgaccttt aatattgtgc ggaataccta ctacggcggc ttcggcaatc ttcggatgcg
     9961 ccaccagcgc cgactctatc tctgccgtcc ccagacggtg accggagacg ttcagcacgt
    10021 cgtccacacg cccggtgatc cagtaatagc catcttcatc gcgacgcgcg ccgtcgccgc
    10081 tgaaatacat atttttgaag gtggaaaaat aggtctgctc aaaacgttcg tgatcgccaa
    10141 acagcgtacg cgcctgacca ggccaggagt cggtgatcac caggctacct tcggtagccc
    10201 cttccagcgg gttaccttcg ttatcgacca gcgccggttg cacgccgaag aacggacgtg
    10261 ttgccgaacc ggctttcagc tcggtagcgc caggcagcgg ggtgatcatg aaaccgccgg
    10321 tttcggtctg ccaccaggta tcgaccaccg gacatttctc gttgccgatt tttttccagt
    10381 accactccca cgcttccggg ttaattggct cgcccacgga accgagaatg cgtagcgacg
    10441 aacggtcggt gccttcaatc gctttatcgc cttccgccat cagcgcgcgg atcgccgtgg
    10501 gtgcggtata gagaatattg acctgatgct tgtccaccac ctgcgccata cgggcaggcg
    10561 tcggccagtt cggtacgcct tcaaacatca gcgtggtcgc gccgcaggcc agcgggccgt
    10621 acagcaaata actgtgtccg gtaacccagc ccacatcggc ggtgcaccag tagatatcac
    10681 ccggatgata atcaaagaca tatttaaagg tcagcgccgc gtacaccaga taaccgccgg
    10741 tagtgtgcag tacgcctttt ggttttccgg tagaaccgga ggtatagaga ataaacagcg
    10801 gatcttcggc gttcatctct tccgcctggt gctgatcgct ggcttgctca accaggtcgt
    10861 gccaccacag gtcgcgccct tcctgccagt cgatgtttcc gccagtacgc ttcagtacca
    10921 ccacatgctc tacgctggtg acgttcgggt tttttagtgc gtcatcaacg tttttcttca
    10981 gcggaatact acgcccggcg cgcacgcctt cgtcggaagt gatcaccagt cgtgagttgg
    11041 aatcaataat gcgcccggca acggctttcg gcgagaagcc gccgaaaatc accgaatgca
    11101 ccgcgccaat gcgggcgcag gccagcatcg caaccgcggc ttccggcacc atcggcatat
    11161 aaatcgccac cacatcacct tttttaatgc ccagctcgag cagggtattg gcgaagcggc
    11221 agacgtcgcg gtgcagctct ttatagctga tatgtttgct ctggctggcg tcgtcgcctt
    11281 cccagatgat ggcggtgcga tcgccgtttt cttgcagatg gcggtcaagg cagtttgccg
    11341 ccagattcag cgtgccgtcc tcgtaccatt taatggacac attaccgggg gcaaaggagg
    11401 tatttttcac cttctggtac ggtttgatcc agtcgagaat ttttccctgt tcgccccaga
    11461 aggtatcagg tacgttaata gattgttgat acatcgcctc gtactgctga gggtttatca
    11521 ggcaacggtc tgcgatgttg gcaggaatgg tgtgtttgtg aatttggctc atgcttttgt
    11581 tctccttgta ggatgttaat aatatgtggc ataagcgtta aatgtagggg tattggcagt
    11641 tttgtttagt atttgggcga cagatcacgc aaaagtagaa tggtgcaaat aaacggcagg
    11701 gtaatttttg aaggtcacca acaaaagttg attaattctt tgaggaacat gcagttatgc
    11761 atgctgttga aaaagaggaa gatactaact aactctaaag tggtatttta catgcactta
    11821 caattgatta aagacaacat tcacagtgtg gttatttgtt acacataggg gtgagcaatg
    11881 tcatgacagt gtaggtgcgg ttactgtcgt gaaaaacaat aaaaaccgcc attgcaacaa
    11941 tggcgcaatt cggatgaagc ccctatgaca aggataaaaa taaacgcacg ccgtatcttc
    12001 agcttattga ttcctttttt ctttttcact tctgttcacg ctgaacaaac ggcagctccc
    12061 gcaaaacctg taactgtgga agcgaagaat gaaacctttg ccccgcagca tcacgatcaa
    12121 tatctctcct ggaaagccac ctcggaacag tcagagcgtg ttgacgccct ggaggaagat
    12181 ccacggctgg tgatcctgtg ggcggggtat cccttctcgc gcgattacaa caagccgcgt
    12241 ggacatgcct ttgctgtgac cgatgtgcgt gaaaccctgc gtaccggtgc gccgaaaaac
    12301 gctgaagatg gtccgctacc gatggcgtgc tggagttgta aaagcccgga tgtggcgcgt
    12361 ctgatccaga aagacggcga agatggctac ttccacggta aatgggcgcg cggcggcccg
    12421 gaaatcgtca acaacttagg ttgtgccgac tgccataaca ccgcctcacc agagttcgcc
    12481 aaaggcaaac cggagttaac cctttcccgt ccgtatgcgg ctcgcgcgat ggaagccatt
    12541 ggtaaacctt ttgagaaagc cggacgtttc gacaagcaat cgatggtttg cggtcagtgc
    12601 catgtggagt attacttcga cggcaaaaac aaagcggtta aattcccgtg ggatgacggc
    12661 atgaaagtcg aaaatatgga gcagtattac gacaaaattg ccttctctga ctggactaac
    12721 tccctgtcga aaacgccaat gctgaaagcg cagcacccgg aatatgaaac ctggacagcg
    12781 ggcattcacg gtaaaaacaa cgtgacctgt atcgactgcc atatgccaaa agtgcagaac
    12841 gccgaaggca aactctacac cgaccataaa attggtaatc cgtttgataa cttcgcccag
    12901 acttgtgcga actgccatac ccaggataaa gctgccttgc aaaaagtggt cgcggaacgt
    12961 aagcaatcga ttaacgacct gaaaatcaag gttgaagatc aactggttca cgctcacttc
    13021 gaagcgaaag cggcgctgga tgcaggcgcg acggaagccg aaatgaagcc gattcaggac
    13081 gatatccgtc atgcccagtg gcgttgggat ctggcgatcg cttcccacgg tattcatatg
    13141 cacgcaccgg aagaaggtct acggatgctc ggtacggcga tggataaagc ggcggatgcg
    13201 cgcaccaaac tggcgcgcct gctggcgacc aaaggcatca cccatgaaat ccagatcccg
    13261 gatatctcga ccaaagagaa agcccagcag gccattggcc tgaacatgga acaaatcaag
    13321 gccgagaagc aggacttcat caaaacggtg atcccgcagt gggaagagca ggcacgtaaa
    13381 aacggtctgt taagccaata accccgttcc gcctcgcaag gggcggaaaa cacaatggag
    13441 tgaatatgag cgtattacgt tcgttattaa ctgctggggt gctggcgtcg ggcctgttgt
    13501 ggagcctgaa cggaattacc gctacccctg cggtgcaggc atctggtgat cgctacgaag
    13561 ttacccagca gcgtaacccg gatgccgcct gtctggactg tcataaacca gataccgaag
    13621 gtatgcatgg caaacatgct tccgttatca acccgaataa caaattgccg gtcacctgca
    13681 ccaactgtca cggccagcca tcaccgcaac accgcgaagg ggtgaaagat gtgatgcgct
    13741 ttaacgagcc gatgtacaag gtcggagagc agaacagcgt ctgtatgtcc tgtcatctgc
    13801 cggaacagtt gcaaaaagcg ttctggccgc acgatgtcca cgtaaccaaa gtggcgtgcg
    13861 ccagctgcca ttccctgcat ccacagcaag atacgatgca gacgttaagt gacaaaggac
    13921 ggattaagat ttgcgtcgat tgccacagcg atcagcgcac caatccgaac tttaacccgg
    13981 cgtctgttcc gttgcttaag gagcagccat gacctggtct cgtcgccagt ttctcaccgg
    14041 cgtcggcgtg ctggcagccg tcagcggcac cgcagggcgc gtggtggcga aaacgttgaa
    14101 tatcaatggg gtgcgttacg gcatggtgca tgacgagtcg ttatgcatcg gctgtaccgc
    14161 ctgtatggat gcttgtcggg aagtgaacaa ggtgccggaa ggcgtctcgc gcctgacgat
    14221 tattcgtagc gagccgcagg gcgaatttcc tgatgtgaaa tatcgcttct tccgtaagtc
    14281 ttgccagcac tgcgagcatg cgccgtgcgt tgacgtctgc ccgaccggtg cgtcttttcg
    14341 tgatgccgct agcgggattg tcgatgttaa tcctgatctc tgcgtcggtt gtcagtactg
    14401 catcgccgcc tgtccgtacc gcgtgcgctt tatccatccg gtcacgaaga cggcggacaa
    14461 atgcgatttc tgtcgtaaga ccaatttgca ggccggtaag cttcccgcgt gcgttgaagc
    14521 ttgcccgacg aaggcgctga cgtttggcaa tctggacgat cctaacagtg agatttcgca
    14581 actgctgcgc cagaagccta cttaccgcta caagctggcg ctgggaacca aaccgaagct
    14641 gtaccgcgta ccgtttaaat acggggaggt gagccaatga cgcagacttc cgcatttcat
    14701 tttgaatcgc tggtgtggga ctggccgatt gccatctacc tgtttttgat tggtatttct
    14761 gccggtctgg tgacgctggc cgtgctgtta cgtcgcttct acccgcaggc gggcggtgca
    14821 gacagtacgt tgctgcgcac cacgctgatt gtcgggccgg gcgcggtgat cctcggtctg
    14881 ttgatcctcg tcttccacct gacaagaccg tggaccttct ggaagctgat gttccactac
    14941 agttttacct cggtgatgtc gatgggggtg atgctgtttc agctctacat ggtggtgctg
    15001 gtgctgtggc tggcgaaaat ctttgaatat gatttgcttg ccctgcaaca acgctggttg
    15061 ccgaagctgg ggatcgtgca aaaggttctg agccttctga cgcccgttca tcgcggactg
    15121 gaaacgctga tgctggtgtt ggcggtgctg ctgggggctt ataccggctt tctgctgtcg
    15181 gcgctgaaat cgtatccgtt cctcaataac ccgagcctgc cggtgctgct cctcttctcc
    15241 ggcatctcgt ccggtgcggc ggtggcgctg atcgccatgg cgatacgcca acgcagtaac
    15301 ccgcattcca cggaagcgca ctttgttcac cggatggaaa tccccgtggt atggggtgaa
    15361 atcttcctgc tggtggcgtt ttttgtcggt ctggcgctgg gcgatgacgg taaagttcgt
    15421 gcgctggtgg cggcattagg tggcggtttc tggacgtggt ggttctggct tggtgtcgcc
    15481 gggctggggc tgattgtgcc aatgttgctc aaaccgtggg tcaatcgcag ttccggcatt
    15541 cctgccgtgc tggcggcgtg tggggccagt ctggtcggcg tgttgatgct gcgctttttc
    15601 attctctacg ccggacagtt gacggtggcg taagccagaa aagaggtggt ttctggacgt
    15661 attccttcct gaagtcggtt ttctggcgtt gttgttaagt ctcggggtca acgtgttgac
    15721 cccgttgacg gcctttgcgg gagtgcggtt gcgctggcct gccatgatgc gactcacttg
    15781 catcggcatt ctggcgcagt tcgcgctcct gctgctcgcc tttggcgtac tgacgtattg
    15841 ttttctcatc agcgatttct cggtcattta tgtcgcccaa catagctaca gcatgctgtc
    15901 gtgggaactc aaactggcgg cggtgtgggg cggtcatgaa ggttcgctgc tgctttgggt
    15961 gctgctgctt tccgcctgga gcgcgctgtt tgcctggcat tatcggcagc aaagcgatcc
    16021 gctatttccg ctgacgctag ccgttttatc tctcatgctc gccgcactgc tactgtttgt
    16081 ggtgctgtgg tccgatccct tcgtgcggat atttccacca gcaatcgaag gccgcgatct
    16141 caatccgatg ctgcaacatc ccggtcttat ctttcatcca ccgctgcttt accttggcta
    16201 tggcggtttg atggtagcgg cgagcgtggc gctggcgagt ctactgcgcg gcgagtttga
    16261 tgcggccagc gcccgaattt gctggcgctg ggcgttacct ggctggagtg cattaacggc
    16321 ggggatcatc ctcggttcct ggtgggccta ctgcgaactc ggctggggcg gctggtggtt
    16381 ctgggatccg gtggaaaacg cctctttatt accctggctt tctgccactg cgctgctgca
    16441 cagtttgtcc ctgacacgcc agcgggggat tttccgccac tggtcgctgt tgctggcgat
    16501 agttactctg atgctgtcgc tgctgggcac cttaattgtc cgttctggca ttctggtttc
    16561 ggttcatgcg ttcgcactgg ataacgtccg cgccgtgccg ttgttcagcc tgtttgcact
    16621 gattagcctt gcgtctttgg ctctgtatgg ctggcgagcg cgggacggtg gcccggcggt
    16681 gcgtttttcg gggttatcgc gggaaatgtt aatcctcgct acgctgttgc tgttttgcgc
    16741 agtgctactg atcgtgctgg tgggaacgct ttatccgatg atttacggcc tgctgggctg
    16801 gggacgcctc tccgttggcg cgccgtattt taaccgcgcg acgttaccgt ttggtctgtt
    16861 gatgctggtg gtgattgtgc tggcgacgtt tgtctctggc aaacgcgtgc agcttccggc
    16921 gctggtagct catgctggcg tgctgttatt tgccgcgggg atcgtggttt ccagtgtcag
    16981 tcgtcaggag atcagcctga atttacagcc gggtcagcag gtgacgctgg caggatacac
    17041 cttccgtttt gagcgactcg atctgcaagc cagaggcaat tacaccagcg aaaaagcgat
    17101 agtggcgctg tttgaccatc agcaacgcat tggtgaatta acgccggagc ggcgttttta
    17161 tgaagcacgc cgtcagcaaa tgatggaacc gtcaattcgc tggaacggca tccatgactg
    17221 gtatgcggtc atgggtgaaa aaaccagagc ggatcgttac gcttttcgtt tgtatgtaca
    17281 aagcggtgtg cgctggatct gggggggagg attgttgatg attgcgggcg cattgttaag
    17341 cggatggcgg gggaagaagc gcgatgaata aagggcttct cacgctactg ctgttattta
    17401 cctgttttgc gcgcgctcag gttgtagaca cctggcaatt cgccaatccg caacaacagc
    17461 aacaggcgtt aaatattgcc agccagttac gttgtccgca gtgccagaat caaaacttac
    17521 tcgaatccaa tgcgccggtg gcggtcagta tgcgccatca ggtttatagc atggtgtcgg
    17581 aggggaagaa cgaagtcgaa atcattggct ggatgaccga acgctacgga gattttgttc
    17641 gctataaccc accgttaacg ggtcagacgc tggtgttgtg ggcgctgcca gtggtgttgt
    17701 tattgctgat ggcactgatc ctctggcgag tgagggctaa gcgatgaaac agcctcaaat
    17761 accggtgaaa atgctgacaa cactcacgat attgatggta ttcctctgtg tcggcagtta
    17821 tctgttaagc ccaaaatggc aggcggtacg tgcggagtat cagcgtcagc gcgatccgct
    17881 acatcagttt gccagccagc aaaccccgga agcgcagctt caggcattgc aggataaaat
    17941 ccgcgctaat ccacaaaaca gcgaacaatg ggcgttactg ggcgagtact atctgtggca
    18001 aaacgattac agcaattcgc tgctggcgta tcgtcaggcg ttgcaactac gtggtgagaa
    18061 cgctgaactg tatgcggcgc tggcgacggt gctttattac caggccagcc agcatatgac
    18121 cgcccagact cgcgcaatga tcgacaaagc cctcgcgctg gactctaacg aaatcaccgc
    18181 tctgatgctg ctggcttccg atgcgtttat gcaggcgaat tacgcgcaag ccatcgaact
    18241 atggcaaaaa gtgatggatc tcaactcacc ccggatcaac cggacacagc tggtcgagtc
    18301 gattaatatg gcaaaactgt tgcagcggag atcggattaa tcatctgttg atgaagatta
    18361 tgtcattgag ctgcataaaa aataatcgaa tgaacatatg ccaaaaataa tcaccaatca
    18421 gtattattgc agattaaaca aataaaaatc tttccataac aaatggttat tcattaatcc
    18481 tgccataaac tcgcgttatt atgcattaat gcagcgaaaa gctctgttgt taaagggttg
    18541 cgcaacatac cgcgcaaatg atactgatca taagcgttaa aaaaatctac aaaccaacgc
    18601 aacacaattc atgccctggc agtatgtcac gttctcgcgt ttctgaacgg ggaacggcgc
    18661 tccattgagg aagttattct gatgaaaaat ataaaattca gcctggcctg gcagattctg
    18721 tttgctatgg tgctgggcat tctcctggga agctacctgc actaccatag cgacagccgc
    18781 gactggctgg tcgtcaattt gctctctccg gcgggtgata tcttcatcca tctgatcaaa
    18841 atgattgttg tgccgattgt gatctccacg ctggtggtgg gtatcgcggg tgttggtgat
    18901 gccaaacagc tcgggcgtat tggcgcgaaa accattattt acttcgaggt gatcaccacc
    18961 gtcgccatca ttttggggat cactctggcg aacgtcttcc agcccggtgc cggggtggat
    19021 atgtcgcagc tggcgaccgt cgatatctcg aaatatcaga gcactacgga agcggtacaa
    19081 agcagttccc acggcattat gggcacgatt ttgtcgctgg tgccgacgaa cattgtggcg
    19141 tcgatggcga aaggcgaaat gctgccgatc atctttttct cggtgctgtt tggtctgggg
    19201 ctttcttccc tgcccgcgac gcatcgtgaa ccgctggtga ccgtgttccg ctccatctct
    19261 gaaaccatgt ttaaagtgac tcacatggtg atgcgttatg caccggtggg tgtgtttgcg
    19321 ctgattgcgg tgacggtggc taactttggt ttctcgtctc tgtggccact ggcgaaactg
    19381 gtgctgctgg tgcatttcgc cattctgttc ttcgcgctgg tagtgctggg aattgtggca
    19441 cgtctgtgcg ggttaagcgt ctggagcctg attcgtattc tgaaagatga gctgattctg
    19501 gcgtactcca ctgccagctc tgaaagcgta ctgccgcgaa ttattgagaa gatggaagcc
    19561 tacggagcac cggcgtcgat caccagcttc gtggtgccga ccggttactc ttttaacctt
    19621 gatggttcga cgctgtatca aagtattgcc gctattttca tcgctcagct gtatggcatt
    19681 gacctgtcca tctggcagga aatcattctg gtgctgacgc tgatggtgac ctcgaaaggg
    19741 attgctggcg tggctggcgt gtcgtttgtg gtgttgctgg caacgctggg tagcgtaggt
    19801 attccgctgg aaggtctggc gtttattgct ggtgttgacc gtatcctcga catggcgcgt
    19861 actgcgctga acgtggtggg taatgcgctg gcggtgctgg tgattgccaa gtgggaacac
    19921 aaatttgacc gcaaaaaggc gctggcttat gagcgtgaag tgctgggcaa atttgataaa
    19981 actgcggatc aataattgaa gattgccggg gatatccccc ggcaatgtgt ggatgcctga
    20041 tgcgacgctt gccgcgtctt atcaggccta ccttaaccgt tggttaattt ttcaaactct
    20101 tcacacccgg tatcaaaccc ttccatacag ctcaggttca accagtgcag cgccttttgt
    20161 ttgttcttct cgataaaacc ttcctcaccg tttaagaaca tcattcccgc ccagtactcg
    20221 gaataaccgg ttcgggaaat tgcagagctg cgtttgaaat accaggttgc cttgtcatcg
    20281 tcggccttaa tgcccacgcc gttggcgtaa atcaaaccaa gcagcatttg ggcatcgacg
    20341 gcggagtcat tctccagatc ttccgaggcg ttttctaaca gcgaaatcgc ctttggataa
    20401 tccggtttac ccgcttgagt atttaccaga atatgcgcca gcgttacttc accttcttta
    20461 ctgcccgctt gcgccgcttt ctctgcaaga acttttgctt gtggatagtc cagactgacc
    20521 ggattggtga ttttgatctg cgccaacagc gcgcaggcat cagcatcacc gctgtcggcg
    20581 gctttctgtg cccaatattc ggctttgctc aaatcgccgg agctaaacca gctgtcggca
    20641 agaaaatatt gtgcgcgttg gtccccggcc tctgctgcct ttaaatactg gctgccaggc
    20701 tcggagtcgt tcgcgtgggc aaagaatgtc aaaaacaaca tcaatgcgat aatttttttc
    20761 attttgataa tacgattggg tagggagtaa ccagtataaa gagttcgaat ggataaaaaa
    20821 acagcctccg aaaggaggct gtagaaagga ctgtattacg ccagtgccgc ttcgcgcagg
    20881 cgagttttca acttgttgta ctcgtcaatc aggtactgct cggcggcgcg ctgatcggag
    20941 atcggctcga cgcgcacggc gcagtatttg tattccggcg ttttcgtaat cgggcttaag
    21001 ttttcggtaa ccagctcgtt acaggcacca atccaccact ggtaggtcat gtaaatcgcc
    21061 cctttgttcg gacgatcgct gacctgcgca cgggtgataa ttttgccttt acgcgagtgc
    21121 actcaaacca atgcctcatc ttcaataccc agacgtttgg cgtcttcggt attgatttgt
    21181 gcgtagccag gttcatcagc cagcgccgcc agtgccgcac agttaccggt catcgaacgg
    21241 caagagtagt gaccaacttc acgcaccgtt gacagtacca tcgggtactc gtcggtgagt
    21301 ttgtcgattg gcgctaccca gtcgcaggtg aagaactgcg ccagaccgtt cggggtatca
    21361 aacttctctt taaacagata agaagtcccc tgatcggcat ctgaagtatc gcggcaaggc
    21421 cactgaatga agcccagttc gcccattttc tcgtaagtcg caccgtagaa atccgggcac
    21481 agatgacgca actcatccca gatctcctgg gtgttgttgt agtgcatcgg ataacccata
    21541 cgggtggcga tttcactgat gatttgccag tccgttttca gatcccattt cggttcaact
    21601 gctttgaaga aacgctggaa gccacggtca gccgcagtaa acacgccttc atgctcgccc
    21661 cacgacgttg acggcaaaat gacatccgcc gccgacgcgg ttttggtcat aaagatgtcc
    21721 tgaacgatga ccagttccag atcttcaaag gctttacgta ctgccgacag ctccgcatca
    21781 gtttgcagcg gatcttcgcc cataatgtac gcggcacgca cttcgccatg cgctgcgcgg
    21841 tgcggcagct cgctgatgcg atagccggta tgagcaggca ggctttccac gccccaggct
    21901 ttggcgaatt tctcgcggtt agccggatct ttcacgtact ggtatcccgg atacgtatcc
    21961 ggcagcgcgc ccatatcgca ggcaccctgc acgttgttct gaccacgaac cgggtttaca
    22021 cccgcatgcg gcttaccgag gttaccggtc agcatcgcga ggctggtcag agaacgcacg
    22081 gtttccacgc cctggtagaa ctgggttaca cccatgcccc acaggatggc ggcgcttttc
    22141 gcctgggcat acatccgtgc cgcctgacga atctcactgg cgctgacacc ggtgatatct
    22201 tcaaccgact ccggcgtgta gccttcaacg attttacgat actcttcaaa gccttctgta
    22261 cgggaagcga cgaacgcttt gtcgtacaga ttttcttcaa taatgacatg gcccatcgca
    22321 ttcaacagcg cgatgttcga gccgtttttc agtgcaatgt gcatgtcagc aatgcgcgcg
    22381 gtttcaattt tgcgcggatc gcagacgata attttcgccc cgttacgttt agcgttaatt
    22441 acgtgattcg ccacgattgg gtgggaatcc gccgggttgt acccgaaaac gaacactaaa
    22501 tcggtattat caatttcgtt aatagcattg ctcattgcgc cattaccgac cgattggtgc
    22561 agacctgcaa ccgatgggcc gtgtcagaca cgagcgcagc agtcaacgtt attggtacca
    22621 ataacggcgc gcgcaaattt ttgcattaca tagttggttt cgttacccgt accacgcgag
    22681 gagccggtcg tctggatggc atccggaccg tacttctctt tgatggcgct caggcgctcg
    22741 gcaacgtaat tcagtgcctc atctcaggaa acaggttcga gtttgccgcc acgctggcga
    22801 cggatcatgg gggttttcag gcgcggggtc aggatctggg tatcgttaat gaaatcccag
    22861 ccataataac ccttcagaca cagggtaccc tggttggttt tcccctgcgc tgcctccgcc
    22921 cggacgattt tgccgttatc gacgaccagg ttgattttgc aacctgatgc gcaatagggg
    22981 caaaccgtga cgactttttt catcggtctc gctccagtta atcaaatcac gcatacgcgc
    23041 tctcgactac agtatgcatc ttttatgcca cattttattt ggggtcattc cctgatatta
    23101 cgggcactat ttattcaaaa ctctgacgaa aaacaggctg tcgtcagttt tgacgtgacg
    23161 aaacgaaata ccgcgtgaca gccatcacgc ggcagacatt ttattttttc tcgacgacgg
    23221 gagccgcctg atacccgccg cccagcgatt tcatcaactg aatgctttgg atcacccggc
    23281 ggctgtccag catcagtaat gacatctctt cggcaagtac tggcaaccgg gcttcagtgg
    23341 cctgtaagcg gctggttaag ccgcgctgat aggcggcctc ggcagcgcgc tgggtgaagc
    23401 gcgttgcttc cacgtgttca gcctgcattt ctcgctcgtc gttgagcgtt tgcagacgcg
    23461 tgccgttgac ggcaacgtca cgcaccgcgt tcagtactga ctggttgtaa cgttcaatca
    23521 tcatgttgct ggcggcgcgc gtgccttcga gattggcatt caaccgtcca ccgtcaaaca
    23581 gcggcaattt cagacccggg atgaagttga actggcgact ggtttttttg aataaggtat
    23641 ctaggtggat ggcatcaagg ccgaaaaacg ctttgatatc aaagctcgga tagaacaacg
    23701 cccgcgcgga atccacctga tctaatgacg cctgaacata ccagcgcatg gcttgcagat
    23761 ccgggcgtct ggcgagcaac tcataagaga gtgtcgccgg aatgccggtc tggactcgtg
    23821 gtaatgccac aggtttgatc tccggcatat cgctggctcc cgcgccaatc aatgcgcgca
    23881 gagattctct cgtttcggtg atttgccctt tgacggcagc aatttgttta tcgaccgcca
    23941 gaatctgtgc ccgcgcgccg tggaaaggca cttgtgcttc cagaccgtgc gccactttac
    24001 tttggtgcgc tttcaccgcg taatcaatca catcgcgagt ttgttctaac agatcgagca
    24061 tctgatagct ggcctgcata ctgtaataaa gctgcgctac gcccgtggtc agcgatagct
    24121 ctactgctgc ggtttctgcc agcgcggcat tatgcgcgcc aatggcggcg gcaaccgctg
    24181 agcgatgcac accccacaaa tcaagatcca gtccggcaaa caaacctact gtggcttccg
    24241 tatagtacgg cccgtccatc cccagtgcgg gcgcatccat cgcataaggg cttaaaaagc
    24301 cgttcgccga gacacgttgg cggttaagca tccctaacgc tgccacctgc aactgtgaac
    24361 cggcatctaa caaatcggcc tgcgattgcg ctttttcttc ccgcagtttc gcttcggcga
    24421 gggtgtgtga accacttagc gtccgttgga tcagggcatc caactgcggg tcattgagtt
    24481 gtttccacca ctgcgcctgc ggccagccgg agctggcaag atgaatatca tcggccagtt
    24541 tgatttgttc cggtttgagc tgttgatgag gcgcagaatc cttacgtacc agggcacagc
    24601 cggaaatcag cgtcgtgctg ccgagaatgc tgcacaacag cagacgtgaa agttgacgat
    24661 tgatcattgc gtggctcctt gtgcctgttc cgtggcgggc gttaatgccg gggctgtcca
    24721 gtctggcaag cgggcgaaaa gctggcagac gtgttgctcc tgttttaata aggtgggcag
    24781 gatggcctgc gaggcgggtg tctcttcaag cgttatttga ggagaacggc tgagtgcggt
    24841 tgccagaccg gcagcgtatt tctccagggc gtcggcaaac tgacctgctc tgtccggctg
    24901 tagtgcgtta tccagcgcct gcgccgaatt ccaggtggca tcccaggcgt gaaggatatc
    24961 gcggccctga cgaataaccg tttgcgaacg ttcaatcagt aaggcgcgtt cttcgctgtc
    25021 cagttgacgc tccagcgcca cgcgttggca catctcttca caggcattaa acgccgcatg
    25081 cagaccgata cgaatttgca gataagtgcg cagagccgtg acttcctgct ggcgtgggat
    25141 ccgcattact ttacttaaca tacccagcgc gccagccagt ttttgcggca gcgtgcgcgc
    25201 ttcactttca ggccagacaa aggtgtaaat catcgcggac accacggtac caatgatgat
    25261 acccagggcg cgatcgcgaa tttccaccag gtcgtacact gggccaaaaa cgttttcgag
    25321 cgtggcgagc gcgaaggtga ccaccatctg tgtgccgata taagaagagc gttcagagct
    25381 ggtggcaatc tatgcgccca acaggaaaat cggtgccagc acaaacagca attcgacaat
    25441 attgtccagc cagggcatga ccagtagcgt gaataacagc gccagaatcg cgccgcaaaa
    25501 ggccccgcca aaacgcagca ccatcttctg gtacgacgaa ccgacatttg gattagcgac
    25561 gatcacgcag gtcagcatac aggtgtgaat gccttcccag tccacgccgc tgtaaaaggt
    25621 gtagcagatc aaacaggcga gcaatgtttt taccgcgtag cgtatatagt ctggattggt
    25681 aaaagcatcg gcgaccattg atggcggttt ggcggcgggc gttggcggcg tattcgggtt
    25741 catctggccc agttgtaaca acgtctggca gatattctcc aggttacatt cccgtgccgc
    25801 caccgcttca ctttcactga tccgccagtc gctttgccag cactgacctt cagcaacggc
    25861 atgctgcagc ttgttgattt ctgaggctaa tttttgccga aattcaataa ttgcctgaga
    25921 atcagcaaaa gaggtgggat cgtagcgatt cagcgtcgag taaatgtagg ttaccgttgc
    25981 cacgcagctt tgccaccatg cgctttgagt tcgccagttg gcatcgtccg cgagacaaaa
    26041 gacattcagt ttttgcagcg ccagcgcctc tctttcaatc cgcgtttcgg gtagcggtgc
    26101 gaggctgtcc gtcaggtggc taatggcatc atcaagcaga tcattaagcg cttgatgcat
    26161 ttgcgtaatg gcacgattgg gaaaccacag cacgccgatt aacgtcatca gcaaggttgg
    26221 atagaggcca acaacgatac accacagcgt taagcgcacg actacttccg gatagtcgag
    26281 catggcgggg aaggtttgcc cgtaaatggc gacaatggcg acagcgaaaa agaccagtcc
    26341 cagccgatgg gtgcgcatca aaaacatgca gcccatcagg atcggcccgg cgatgatcaa
    26401 tcggatcaac ggttcgccgt atgaccattt gtagatcaaa aacaggctgc cgatctccag
    26461 caccgtggca accacaaaca agatcgccac aaatttggtg taaaacgcgt tcgactgaat
    26521 accgtaaaac agcactgcca gcgataacgc cacaaaaggg atctcaaaag tcatcgagat
    26581 cagaatcacc agcaggcagc ctacccagag ttgcacgatc tgcggcacgc ggccgggtcg
    26641 ccgctcgctt aactcttcat gaaagaacgc cagcagcctg accaccggta atggcaggga
    26701 gttgagcgcg ctcattattg cggctcaaga ttagcgactg ccgaagcgcc gatgcggaac
    26761 atttccgggt caggtttatc gaccatgatt ttgaccggaa aacgctgggc aacgcggacc
    26821 cagttaatag aacgagacac ttttggcagg ccgcccagca ccaggccgcc gtcatccggt
    26881 agcacgccgt agccaatcga atccacttta ccctcgaagg ttttgccgct gtcactcatc
    26941 aggcgaatcg ttgcgggtgt acctgagcga atatttttca gatcggtttc gcggaagttg
    27001 gcgatcacgt accagtgacg agtgtcgatg agggtaaaaa tagggcgcat ggcagaagca
    27061 aattgcccga cggaggtttt gagggaaatg atccggccat caaatggcgc gcgaacggtc
    27121 gtcatttcca gatgtagttt ggtcagggca atatccgctt ctgcggcgcg ctgcgccgtt
    27181 cttgcacggt caacgtcttc tgctgagaca aaaccctctt tcagtaatgg ctcggtgcgg
    27241 cgtaatgtat ctgtggcctg tttcgcggcg gcacgggctt tttctaccgt ggcattaacc
    27301 gagtcggcac caaactgttg ggcgtcaacg ctacgctggg tgagcataat ttgcttatcc
    27361 agcgccgcga gggaggcttc ggctttcgcc aggttggctt cgtacgggcg cgggtcgatg
    27421 cggaacagca aatcgccctg tttcaccgcc tggttgtcgg tgaccgccag ttctacaatg
    27481 cgaccgctga cttccggcac cacatcaatg gtatctgctg acgcgtaagc gtcattagtt
    27541 gatggcgcac tgtctacgcg ccagataacg aaaacaaggg caaccagcgc caacgcaacc
    27601 actaacagag cagggaattt actgcgaggt gcttttttcg gcgtactttc catgagcgcg
    27661 tctcaaatag attagaaaaa tgccagccag aacagcatgg cgtagagagc aaaaagggcg
    27721 gtataaataa ttccgacaaa tgccagattg atatttgccc gctgaataac tcgcctcgtg
    27781 atgagcgtca aaataaggct ggcaatggcg cagaaaaacc agctgggata ataagcgccg
    27841 atcaccggga ttgccggaga taatgagcat cccgaaacca tgaagacggg aattatccag
    27901 gctgtttttt tgaggtgtat cgccatgcga gagagaactg ttggcatatt aaatacatcc
    27961 tgtctgctgc gaacaatatc tgtagtgaat atcaatatcc attgaaggcg tcagtatggc
    28021 tattttctcg atgaataaaa atagctttat ccagccaccg cattcataac ttatatattt
    28081 tatttattaa ttggctgtat atatttttaa aataaatgct tttgggggct tcagcgcccc
    28141 caaaagcgag tgacattaat ttggggttac gatattcacc cacaaatcga aattatccag
    28201 acaggcgata atttcttcca gtttcgcgcc attgccaata attttggctc tcttcgcttt
    28261 caccagatcc gcc
//
LOCUS       DADMGC010000041        28025 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.041, whole genome shotgun sequence.
ACCESSION   DADMGC010000041 DADMGC010000000
VERSION     DADMGC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.041"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(98..448)
                     /gene="ubiK"
                     /locus_tag="JNZ73_001759"
     CDS             complement(98..448)
                     /gene="ubiK"
                     /locus_tag="JNZ73_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HAY8732064.1"
                     /translation="MTYPYRTTMVLNTYQYRETTMIDPKKIEQIARQVHESMPKGIRE
                     FGEDVEKKIRQTLQAQLTRLDLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTE
                     IKKQPDPETLPPTL"
     regulatory      510..658
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            762..1415
                     /gene="ribB"
                     /locus_tag="JNZ73_001760"
     CDS             762..1415
                     /gene="ribB"
                     /locus_tag="JNZ73_001760"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HAY8732065.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            1676..1846
                     /locus_tag="JNZ73_001761"
     CDS             1676..1846
                     /locus_tag="JNZ73_001761"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732066.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(1904..2677)
                     /gene="zupT"
                     /locus_tag="JNZ73_001762"
     CDS             complement(1904..2677)
                     /gene="zupT"
                     /locus_tag="JNZ73_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAY8732067.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKTSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            2793..3608
                     /gene="ygiD"
                     /locus_tag="JNZ73_001763"
     CDS             2793..3608
                     /gene="ygiD"
                     /locus_tag="JNZ73_001763"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HAY8732068.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLV
                     ELLAPIPVTLDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(3646..4806)
                     /locus_tag="JNZ73_001764"
     CDS             complement(3646..4806)
                     /locus_tag="JNZ73_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY8732069.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQCGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(4812..5483)
                     /locus_tag="JNZ73_001765"
     CDS             complement(4812..5483)
                     /locus_tag="JNZ73_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HAY8732070.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(5631..7112)
                     /gene="tolC"
                     /locus_tag="JNZ73_001766"
     CDS             complement(5631..7112)
                     /gene="tolC"
                     /locus_tag="JNZ73_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HAY8732071.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQACLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7317..7946
                     /gene="nudF"
                     /locus_tag="JNZ73_001767"
     CDS             7317..7946
                     /gene="nudF"
                     /locus_tag="JNZ73_001767"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HAY8732072.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            7947..8369
                     /locus_tag="JNZ73_001768"
                     /pseudo
     CDS             7947..8369
                     /locus_tag="JNZ73_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
     gene            8394..9221
                     /gene="cpdA"
                     /locus_tag="JNZ73_001769"
     CDS             8394..9221
                     /gene="cpdA"
                     /locus_tag="JNZ73_001769"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HAY8732073.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            9221..9802
                     /gene="yqiA"
                     /locus_tag="JNZ73_001770"
     CDS             9221..9802
                     /gene="yqiA"
                     /locus_tag="JNZ73_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HAY8732074.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            9831..11723
                     /gene="parE"
                     /locus_tag="JNZ73_001771"
     CDS             9831..11723
                     /gene="parE"
                     /locus_tag="JNZ73_001771"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAY8732075.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(11771..12085)
                     /locus_tag="JNZ73_001772"
     CDS             complement(11771..12085)
                     /locus_tag="JNZ73_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY8732076.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSKAVKGDVLEMNIRILQP
                     GI"
     gene            complement(12116..12697)
                     /gene="mdaB"
                     /locus_tag="JNZ73_001773"
     CDS             complement(12116..12697)
                     /gene="mdaB"
                     /locus_tag="JNZ73_001773"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HAY8732077.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            13016..13348
                     /locus_tag="JNZ73_001774"
     CDS             13016..13348
                     /locus_tag="JNZ73_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HAY8732078.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            complement(13394..14743)
                     /gene="qseC"
                     /locus_tag="JNZ73_001775"
     CDS             complement(13394..14743)
                     /gene="qseC"
                     /locus_tag="JNZ73_001775"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HAY8732079.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(14740..15399)
                     /gene="qseB"
                     /locus_tag="JNZ73_001776"
     CDS             complement(14740..15399)
                     /gene="qseB"
                     /locus_tag="JNZ73_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HAY8732080.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            15551..15943
                     /gene="ygiW"
                     /locus_tag="JNZ73_001777"
     CDS             15551..15943
                     /gene="ygiW"
                     /locus_tag="JNZ73_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HAY8732081.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESVKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            15996..16478
                     /locus_tag="JNZ73_001778"
     CDS             15996..16478
                     /locus_tag="JNZ73_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8732082.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            17024..19282
                     /gene="parC"
                     /locus_tag="JNZ73_001779"
     CDS             17024..19282
                     /gene="parC"
                     /locus_tag="JNZ73_001779"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAY8732083.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNARAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            19516..20253
                     /gene="plsC"
                     /locus_tag="JNZ73_001780"
                     /gene_synonym="parF"
     CDS             19516..20253
                     /gene="plsC"
                     /locus_tag="JNZ73_001780"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HAY8732084.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            20328..21740
                     /gene="ftsP"
                     /locus_tag="JNZ73_001781"
     CDS             20328..21740
                     /gene="ftsP"
                     /locus_tag="JNZ73_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HAY8732085.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            21851..23893
                     /locus_tag="JNZ73_001782"
     CDS             21851..23893
                     /locus_tag="JNZ73_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005071065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAY8732086.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDCVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSPRLLSACANH"
     gene            23874..24068
                     /locus_tag="JNZ73_001783"
     CDS             23874..24068
                     /locus_tag="JNZ73_001783"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein"
                     /protein_id="HAY8732087.1"
                     /translation="MPAPTIEEMREARRQNRNTRPALTKHTPMATQRQTPATAKKASS
                     TQSRPVNAGAKKRPKAAVGR"
     gene            complement(24111..24368)
                     /gene="yqhH"
                     /locus_tag="JNZ73_001784"
     CDS             complement(24111..24368)
                     /gene="yqhH"
                     /locus_tag="JNZ73_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HAY8732088.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24419..25345)
                     /locus_tag="JNZ73_001785"
     CDS             complement(24419..25345)
                     /locus_tag="JNZ73_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HAY8732089.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNNNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKLIEAKTAARLKQ"
     gene            complement(25545..26372)
                     /gene="dkgA"
                     /locus_tag="JNZ73_001786"
     CDS             complement(25545..26372)
                     /gene="dkgA"
                     /locus_tag="JNZ73_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HAY8732090.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKCPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            complement(26477..27640)
                     /gene="yqhD"
                     /locus_tag="JNZ73_001787"
     CDS             complement(26477..27640)
                     /gene="yqhD"
                     /locus_tag="JNZ73_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAY8732091.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYKAAR"
     gene            27834..>28025
                     /locus_tag="JNZ73_001788"
     CDS             27834..>28025
                     /locus_tag="JNZ73_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8732092.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYIN"
BASE COUNT         6567 a         6946 c         7441 g         7071 t
ORIGIN      
        1 gtgatgatac tctgaatggg attaagcgat aatgtaatag aaaacactgt aaattagagg
       61 attaattcat aatttacagt gcattaaaga caaatgttta cagcgttggg gggagagtct
      121 ctggatctgg ctgctttttg atctcagtac tgcgattttc cagttcgctg attctttgtt
      181 caagcagcgc cagtttctca cgagtccgta acaggacttg cgtttggacg tcgaactctt
      241 cacggcttac aaggtcgagg cgcgtcagct gagcttgtag ggtttggcgg atttttttct
      301 ccacatcttc cccgaactcc ctgattcctt taggcattga ttcgtgaacc tggcgagcga
      361 tttgctcaat ttttttcggg tcaatcattg tagtttccct gtactgatag gtgttgagta
      421 ccattgtagt gcgataaggg taagtcataa accagaatta tgtgaagcta tgcgttgctg
      481 ccgctaatca ttagagttat agtgaatccg cttattctca gggcggggcg aaattcccca
      541 ccggcggtaa atcaactcag ttgaaagccc gcgagcgctt tgggtgcgaa ctcaaaggac
      601 agcagatccg gtgtaattcc ggggccgacg gttagagtcc ggatgggaga gagtaacgat
      661 tctgtcgggc atggacccgc tcacgttatt ttggctatat gccgccactc ctaagactgc
      721 cctgattctg gtaaccataa ttttagtgag gtttttttac catgaatcag acgctacttt
      781 cctcttttgg tacgcctttc gaacgtgttg aaaatgcact ggctgcgctg cgtgaaggac
      841 gcggtgtaat ggtgcttgat gatgaagacc gtgaaaacga aggcgatatg atcttcccgg
      901 cagaaaccat gaccgttgag cagatggcgc tgaccattcg ccacggtagc ggtattgttt
      961 gcctgtgcat cactgaagat cgccgtaaac aacttgatct gccaatgatg gtagaaaata
     1021 acaccagcgc ctatggcacc ggttttaccg tgaccattga agcggctgaa ggtgtgacta
     1081 ccggtgtttc tgccgctgat cgtattacga ccgttcgcgc agcgattgcc gatggcgcaa
     1141 aaccttctga tctgaatcgc ccgggccacg ttttcccact tcgcgcgcag gcaggtggtg
     1201 tactgacgcg tggcggtcat actgaagcga ctattgattt gatgacgctg gcaggcttta
     1261 aaccggctgg tgtgctgtgt gagctgacta atgacgatgg cacgatggcg cgtgcaccag
     1321 agtgcattga gtttgccaat aaacacaata tggcgctcgt gactattgaa gacctggtgg
     1381 cataccgtca ggcacatgag cgtaaagcca gctgaaaacc gctgcttaat tactgcctta
     1441 atcaagaaac cgaagttgta gctggcttcg gtttttattt tgccctgcta tgcaagatgt
     1501 taattccgct tctctttcta tgagaaaatt tcattaatat caggcattct ttttcattat
     1561 gatcaaaatc atataattgt tccgttatta tgcgaccata tgaatatatc cgaaaaagga
     1621 tatgtgaaat ttatgcgcta aatgtttagt attgttaaat attaagggta aaaggatgtt
     1681 tatcgcctgg tactggattg tattgattgc tctggttgtg gtgggttatt tcctgcattt
     1741 gaaacgttat tgtcgggcgt ttcgccagga cagagacgca ctgcttgaag cgcggaacaa
     1801 atacttaaac agtacgagag aagagacagc cgaaaaggta gaataaggct ttcgtgaagc
     1861 tggcagaagt tgtccatgtg ctggacaact tctgatgttg tttttaacca attcccgccg
     1921 tttgtagcag cactaaactg aatcccatca ctgacattcc acatagtacg ccatagctgg
     1981 ggttattatt agggtcaatc tctttggcga gcggcattaa ttcatcaacc gagagcgcca
     2041 ccataattcc tgcaaccgcc gccatgattg ccgccatgac caccggggaa atcatgctac
     2101 cgaggattaa ccacgccagc acaccaccaa gaatttctgc tagtccagaa atccccgccc
     2161 acagaatcgc ggtgcgttta gaccccgttg ccgcataaac cgggcctgcc actgccagac
     2221 cttcagggat attgtgcaac gcgacggcca gtgcgatgcc aaatcccagc tccaggttgc
     2281 tgctcgccgt gacaaaggtg gcaatccctt ccgggaagtt atgcagactg atgccgagag
     2341 tgagcagaat ggctgtgcgc ttgatcgatt ttggcaacag ctgcaccgat ttttgcatta
     2401 aatcctgcgg atgcgcatgc ggcaacatgc ggtccaggcc aaagtagcca agcagaccga
     2461 agataaacat tccataaccc aacaccggcg acattccttc agctgctagt gcggcaggaa
     2521 gcatttccat taatgagatg agcaacatga tccccgccgc aaaacctagc gaaaacgcca
     2581 gtaagcggtt cgaggttttt tgtccgagaa cgccgagaaa cgcgccaata aacgtggctg
     2641 cccccgccag tatggtcaga atgagaggta ctgacatcga caactcctta taaatcttca
     2701 ccttcaggca gatcgtcaaa ataactgatg atcatcatca ttgttatccg ggttcttact
     2761 ccgccgaaca agcgtattgt gaggatatca aaatgacacc tttagttaag gatatcatca
     2821 tgtcttcaac acgtatgcca gcattgtttt taggtcacgg tagtccgatg aacgtgctgg
     2881 aagataattt gtatacccgc agctggcaga agttggggat gacattgcca cgcccgcaag
     2941 cgattgtggt ggtttcggct cactggttta cccgtggaac aggagtgacc gcgatggaga
     3001 cgccgcccac gattcatgac tttggtggct tcccgcaggc gctgtacgat acgcattatc
     3061 ctgctccggg ttcgcctgcg ctggcacagc gtctggttga gctgttagcg ccgatcccgg
     3121 tgacgctgga taaagaagcc tggggctttg accacggctc gtggggcgtg ctgattaaga
     3181 tgtatcctga cgccgatatc cctatggtgc agttgagtat cgacagtagc aaacctgccg
     3241 cctggcattt cgaaatgggg cgcaaactcg cggcgttgcg tgatgaagga ataatgttgg
     3301 tcgccagcgg taacgtggtg cataacctgc gcacagtgaa gtggcacggt gatagttcac
     3361 cgtatccgtg ggcgacgtcg tttaatgagt atgtgaaagc gaatctgacg tggcaagggc
     3421 cggtagagca gcatcctctg gtgaattatc tcgaccatga aggtggcgcg ttatcgaacc
     3481 caacgccaga gcactatctg ccgttgttgt atgtgttagg tgtgtgggat gggcaggagc
     3541 caattaccat tccggtcgat ggtatagaaa tgggcagttt aagtatgctg tcggtgcaga
     3601 taggctaaca attaccggat ggcgagtgcc atccggtatc gtggcttatt caacaaaaat
     3661 atgtggataa aaccgagaca tatcctgggt gatcaacgca cggtcttcac gaatgccaat
     3721 tccggcaggt tgatcgttca ccagccagct accaatcagc atatagctgt cgccgaattt
     3781 cggtaacggg tggaattgct gaacaatcat cccttcttcg ccatacggac cttccgctgc
     3841 ttcaatggtt ttgccgttct caatgatcga cacgtttgcg ccttcacggg agaagatcgg
     3901 tttaaccaca tatttttcca tttgcggatg atcatcttcc gcaaaataag cgggcagcag
     3961 gttcgggtga ttcgggaaca tctcccacag tagcggtaga agtgccttgt tggagataat
     4021 actcttccac gccggttcca gccagcgtac gccagcatct tccagcttgg tggagaacat
     4081 ttcgcgcagc ataaattccc acggataaag cttgaacagg ttagaaatca cctgatcctg
     4141 taaatccgtg aactggcctt tttcgcctaa acctatctcg tcgatgtaga ggaactcggt
     4201 ggcgatttca gcttcggttg cgcagtcctg caaatactga atggttccgc gatcttccac
     4261 cgtgtcgcga cagcaggtga gatgcagcaa ctggaagcca cactgttcac gcagctcaac
     4321 gaagcgatcg atcagttttt cttgcagact gttaaactgg tcgctgccct ccggcaagtt
     4381 accggcgtta agctgatctt ccagccagat ccactgaaag aacgccgcct cgtatagtga
     4441 cgttggcgta tcggcgttat tttccagaag tttaggttcg ccagtgccat cccacgccag
     4501 atcaagacgc gaataaagcg atggctggtg agtcagccat gattgacgca caaaactcca
     4561 ggtgtgtttt ggtatgcgga atttggtcat cagctcatcg ctggcgatca ctttttccac
     4621 cactttcagg cacatctggt gcagttcggc ggtgacttct tccagctttt caacctgggc
     4681 gagggtcaac ttgtagtaag catcttcaca ccagtacggc tcgccgtaca tggtgtgaaa
     4741 attgaaaccg tattcgtggg ctttctcacg ccagtccggg cgctcggtaa tactgactct
     4801 ttccatcggt atcagccacc cattgaacga gaagaggtgc cggttgcgct acgctgcata
     4861 gtgctttgtt tggcaacaga ttcaccaaaa ccgccacggg taacggtagt ggtggtcgcc
     4921 ggttttggtg ccattgccgt cttcggtacg gtcatggtgc ggcctggctg ggctgcgcca
     4981 tagtttttac ccgtcgcgtc ggtatattta ccgtaagccg gactggccgg gtttttcgag
     5041 gagaacagcg gctgctgtgc aaatcccgcg ccgccgccca tcagacgccc catcatgtaa
     5101 ccggccatca gcggcatcca gaaactcccg ctggattgct gagcctgcgc ctggttttct
     5161 ggtgccatgc cagcctgggc tggtgcttgc tgacactgac cttcaccaaa ttcagcaaca
     5221 cagtcttcac gggtggcgta tttcggcgca gtacgttcgg cttctttcag cgcattgttg
     5281 tacgcggtgg tacattcggc gcttttgcct gggtttgcag ctgaacagtc gtcagcattt
     5341 tgatagagag acactgtttc atcactcttt tcacagccag ccagcataaa aacagtggca
     5401 accgcgagag cgactggtgt cagatggcgt gcgctccagt ttttgcggaa cgatgcgtgg
     5461 cgtatggatt ttgtccgttt catttttgtc ttccgggacc agtggtaaat acccatcaga
     5521 atagaggatg gctggtcgaa attgaagcga gaagggggaa gaatgcggca gataacccgt
     5581 atctttacgt tgccttacgt tcagacgggg ccgaagcccc gtcgtcgtca tcagttacgg
     5641 aaagggttat gaccgttact ggtggtagtg cgtgcggatg tttgctgaac gacgggtgcc
     5701 gggctatcag gcgcataacc atcagcaata gcattctgtt ccggcgtttg cggggcaacg
     5761 ttttccggat tagtggaaac cggtttgctc agcgcattgt tcagtgccag caaatcctgc
     5821 tcgttcaacg tacccagggc tgacttaata ttcagctgat taatcaggta gttataacgc
     5881 gcattcgcca gctcttgctt ggcgttgtac agcgtggtgg tcgcatccaa cacatcaaca
     5941 atggtacgcg taccgaccga gtagcccgct tccatcgcgt ctaatgagct ttgcgcggaa
     6001 actacggctt gtttgtaggc gttaatacta ctgatagaag cattaatgtt gttgaaggag
     6061 gaacgtacgg tttgcacgac gctacgatgt gcgctttcca gttgctcact ggcaccaaca
     6121 aagttgtact gtgcctgttt cacctgcgag ttaaccattc cgccctgata aatcggcagc
     6181 gagaagctca ggccaacttt gttctggccc atattgctat cgtcatactg ggtaccagcg
     6241 gcaccacggg ttttcgaacc gctataagag gtgtcagaaa tcccggtaga agccgttaaa
     6301 tccagagtcg gtaagtgacc atcctgcgcc tggcgaattt gctcgcgcgc caggtcctgg
     6361 ctcaagcatg cctgtaacag cgacaggttg cgtttttcgg cttctttcag cagcgcgtta
     6421 accggctgtg gtttgtcggt tttaaagttt tcgacattca gcgccgccag ttccggatag
     6481 tagttaccgg tgatctggcg cagctgctct accgcgttat caaggttatt acgtgcggtc
     6541 acttcgttcg ccagcacggt atcgtactgc gcgcgggcgt tctgcacgtc ggtgatcgct
     6601 accaggccca cgttaaaacg ttgggtggtt tgatctaatt gacggtagat cgcttctttt
     6661 tgtgcctgtg tataggaaag aacgtcaata gcattcaaca cgttgaaata agcggtcgcg
     6721 gtgttgagga tcaaggtttg ctgatcggtc tgatatgtga cgtcctgaat ccctgctgct
     6781 ttttcctgca gcgttaacgc acgccatttc gacatatcaa aaatggattg agttaactgc
     6841 agggacgcac tggtcgcgtt cgagttgatg ccgttcgcgt cgcggtagcc gttgctatag
     6901 gtgtaatctg cacctaaacc tagctgtggc agtaatggac tgcgcgcttc attaattttt
     6961 tcaaaggcag catcacgatc ggcggcagac ttacgcaatt ccgggttact aaggcgtgct
     7021 tgctgataaa cttgcatcag gttctcggcc tggctcaacg aactgaaccc agaaaggctc
     7081 aggccgataa gaatggggag caatttcttc atttgcattc cttgtggtga agcagtattt
     7141 agcgcgatca aactgtaaaa ttattgcgga ttctagcaga agccgctacc gcaaaaggtt
     7201 ggcgttacgt gccatagggc gttaatgtgc ctcaatttaa catatgactg ctcaaacggc
     7261 agtcaaacgt cgctgaaatt cacatttaat tcactattag tgccaggaca ttaacaatgc
     7321 ttaagccaga caacctgccc gttacatttg gcaaaaacga tgtagaaatt attgcacgag
     7381 aaacacttta tcgcggcttt ttttcattag atctttatag atttcgtcat cgtctattca
     7441 acgggcaaat gagtcatgag gtacggcggg aaatttttga gcgcggtcac gccgcagtct
     7501 tgctaccctt tgacccagtg cgtgatgaag ttgtgctgat tgagcagatt cggattgccg
     7561 cgtacgacac cagcgaaacc ccctggctac tggagatggt tgccgggatg attgaagagg
     7621 gtgaaagtgt ggaagatgtt gcccgtcgcg aagcgattga agaggcggga ctgatagtca
     7681 aacggaccaa accggtgtta agtttcctgg caagcccggg gggcaccagt gagcgttcgt
     7741 caattatggt gggcgaagtg gacgccacga ccgcaagcgg tattcatggt ctggctgatg
     7801 aaaacgaaga tattcgcgtt catgtggtaa gccgggaaca ggcataccag tgggtagaag
     7861 aggggaaaat cgacaacgca gcgtcggtca tcgctttgca atggctgcag ctgcatcatc
     7921 aagcgttaaa aaatgagtgg gcataaatga agcgttacac acctgacttt cctgaaatga
     7981 tgcgcctgtg cgagatgaac ttttcataat tgcgccgttt gttaccgcgc aattacgcac
     8041 ccggcgaaac tgtaagctat caggtggcaa acgcacaata tcggctgacg attgtggaat
     8101 cgacccgata cactaccctg gtgacaatag aacagactgc gcccgcgatc agttactgga
     8161 gccttccgtc aatgacggtg cgtctgtatc atgacgcgat ggtggctgaa gtgtgttcaa
     8221 gccagcagat ttttcgcttc aaagcgcggt atgattatcc taataaaaag ttgcatcaac
     8281 gcgacgaaaa gcatcaaatt aaccagtttt tagcggactg gttgcgatac tgtttagcac
     8341 atggagcgat ggcgattccg gtttactagc gtcgtgaaac ctaaggacac catttggaaa
     8401 gcctgttaac ccttcctctg gctggtgagg ccagaggaag gattttacaa attaccgata
     8461 ctcacctgtt tgcacaaaag cacgaagccc tgttaggggt aaacacctgg gaaagctacc
     8521 aggcggtgct ggaggcgatt cgtccacacc agcacgaatt cgacctgatt gtcgcgacag
     8581 gtgatttagc gcaggatcaa tcctctgcgg cctatcagca tttcgctgaa ggcatcgcaa
     8641 gttttcgtgc gccctgcgtc tggctgccgg gcaaccacga tttccagccc gcgatgtaca
     8701 gcgcgttaca ggatgcgggt atctccccgg cgaagcgggt gtttattggt gagcaatggc
     8761 aaatcctgtt gctggatagc caggtgtttg gcgtgccgca cggtgagctg agcgagtttc
     8821 agcttgagtg gctggaacgt aaactggccg atgcgccaga acgccatacg ttgctgctgc
     8881 tgcatcatca tccgctacct gcgggttgta gttggctcga tcaacacagt ctgcgtaacg
     8941 cgggcgaact ggataccgtg ctggcgaagt ttccgcacgt caaatacttg ctgtgcggtc
     9001 atattcatca ggagctggat ctcgactgga acggtcgccg cctgctggca acgccgtcga
     9061 cctgtgtgca gtttaagccg cactgttcca actttacgct ggatactatc gcgcccggct
     9121 ggcgtactct cgagttacat gctgatggca cgctgaccac cgaggtgcat cgcctagcgg
     9181 acacacgttt ccaacctgat accgcttcag aaggctactg atgtctacgc ttctttattt
     9241 acacggtttc aacagctcgc cgcgctctgc aaaagcgagc ttgttaaaaa actggctggc
     9301 ggaacatcac cctgacgttg agatgatcat tccgcagttg ccgccgtatc cttccgacgc
     9361 ggcagaactg ctggaatcca ttgtcctgga acatggcggc gattcgctgg gtattgtcgg
     9421 ttcgtcactg gggggatatt acgccacctg gttgtcgcaa tgttttatgc tgcccgcagt
     9481 ggtggtaaac ccggcggtac gcccgtttga attgctgacg gactatctcg gtcagaacga
     9541 gaacccctac accgggcagc aatatgtgct agagtcacgc catatttacg atctcaaagt
     9601 catgcagatt gacccgctgg aagcgccgga tttgatctgg ctgctgcaac agacgggaga
     9661 tgaagtgctg gattaccgcc aggcggtggc gtactacgct tcctgccgcc agactgtcat
     9721 agaaggcggc aaccacgcat tcacgggctt cgaagattat ttcaacccga tcgtcgattt
     9781 tcttggtctg caccatctct gacgatcaac tcgaattgct aacttaaacc atgacgcaaa
     9841 cttataacgc tgatgccatt gaggtactca ccgggcttga gccggttcgc cgccgtccgg
     9901 ggatgtatac cgataccact cgccctaacc atttggggca agaagttatt gataacagtg
     9961 tcgatgaagc actggcgggt cacgcaaaac gcgtggatgt aatcttacat gctgaccagt
    10021 cgttagaagt gattgacgat gggcgcggga tgccggtgga tattcacccg gaagaaggag
    10081 taccggcggt tgaactgatt ctttgccgtc tgcatgcagg cggtaaattc tctaacaaaa
    10141 attaccagtt ctctggcggc ctgcatggcg tggggatttc ggtagttaac gccttgtcga
    10201 agcgcgtaga agttaacgtg cgccgcgatg gtcaggttta taacatcgcc tttgaaaatg
    10261 gcgaaaaggt gcaggattta caggttgtcg gcacttgcgg taaacgcaat accggtacca
    10321 gcgtgcactt ctggccggat gaaaccttct ttgacagccc gcgtttttct gtttcacgcc
    10381 tgacgcatgt gctgaaggcc aaagcagtac tgtgccccgg cgttgagatt acctttaaag
    10441 atgagatcaa caacaccgaa cagcgctggt gctatcagga cggtctgaat gattacctgg
    10501 cggaagcggt aaacggttta ccgacgctgc cagaaaaacc gtttatcggt aatttcgctg
    10561 gcgatactga agctgtggac tgggcgctac tgtggctgcc ggaaggcggt gaactgctga
    10621 ccgaaagcta cgtcaacctt atcccaacga tgcagggcgg tacccatgtt aatggtctgc
    10681 gtcagggcct gttggacgcg atgcgtgagt tctgtgaata ccgcaacatt ctgccgcgcg
    10741 gcgtgaagct gtcggcggaa gatatctggg atcgctgcgc ctatgttctg tcggttaaaa
    10801 tgcaggatcc gcagtttgcc gggcagacca aagagcgtct ctcttcgcgt cagtgtgcgg
    10861 cattcgtttc gggcgtggtg aaagatgcct tcatcctgtg gctgaaccag aacgttcagg
    10921 cggcggagtt gctggcagag atggcgattt ccagcgccca gcgtcgtatg cgtgcggcta
    10981 aaaaagtggt gcgtaaaaag ctgaccagcg gcccggcgct gcctggcaaa ctggctgatt
    11041 gtaccgcgca ggaccttaac cgtaccgaac tgttcctcgt ggaaggtgac tccgcaggcg
    11101 gatctgccaa gcaggcgcgc gatcgtgaat atcaggcgat catgccgctg aaaggtaaga
    11161 tccttaacac ctgggaagtt tcttccgacg aagtgctggc ctcgcaggaa gtgcatgata
    11221 tttcggtagc gatcggtatc gatcctgaca gcgacgatct gagccagctt cgttatggca
    11281 aaatctgtat cctcgcggat gcggactctg atggtctgca cattgccacg ctgctctgcg
    11341 ctttgttcgt aaaacacttc cgcgcgttgg tgaaacacgg tcacgtttac gtcgcactgc
    11401 caccgctcta ccgtattgat ctcgggaaag aggtttatta cgcgctgacg gaagaagaga
    11461 aagagggcgt acttgagcaa ttaaaacgca agaaaggcaa gccgaacgtc cagcgtttta
    11521 aaggtctggg ggaaatgaac ccgatgcaat tgcgtgaaac cacgcttgat ccgaacactc
    11581 gccgtctggt gcagttgact atcgatgatg aagacgatca gcgtactgac gcgatgatgg
    11641 atatgctgct ggcgaagaaa cgctcggaag atcgccgcaa ctggttgcaa gagaaaggcg
    11701 acatggcgga gattgaggtt taaaggaaag aacattgccc ggtcaggatg gccgggcaaa
    11761 caaggcagga ttaaatccct ggctgcagaa tacggatatt catctccagc acgtcgcctt
    11821 ttacggcttt gctgtacgcc ttcatgtgcg gggtttgcag atgcgcttca aggtgcgcga
    11881 tgctttccca ctgctcaatc atcacgatag aatccggtgc catagactgg aaactcacgc
    11941 cagcagcgca atccaccatt ggcgcatagc cgtggcaacc ttcttctttc agtacggttg
    12001 gaacgatttt agcaaactga tccaataccg cctgacggtg atgttgacca ggacgagtac
    12061 ggatttctgc gattacggta agcatggtta actccttcta aagcctgagc tctagttaac
    12121 caaaaatttc cacaagatgc ttgcgatatt cttcagtata gcggggaaca tcaggcattt
    12181 ttatcacgtc attagcgata aatgtcggca gcggttccat accgaggaat tggtttgctt
    12241 tatggaacgg cagatacaca ccgtcaacgc caacgccgtg gaagaactga tctttttcgg
    12301 tgaaggcttc cattggtgcg ttccaggtta gagaaagcat atattttttg ccctgtacca
    12361 ggccgccgga accgtatttt ttcgacggat ctttgcgggt acgaccatcg ctggcataca
    12421 gagtcccgtg accttcggtg aatacatcat caatatattt tttcactgtc cacggcgcgc
    12481 ccatccacca gcctggcatc tgccagatca ccacatcagc ccagagaaag ttttgtactt
    12541 ccgctttgac attgtagtcg ctgtcggcgc gaacgatgcg gacatcatgc ccgaggtcgc
    12601 gcagtgtgcc atccgcgact tcggtcaggg tgtcgttcag ttgaccattg gagtgggcga
    12661 attttttcgc gccgttgata atcaggatgt tgctcatttt ttaacctcaa ggtgagacgt
    12721 ttgtcgacta tagtacgtca cagagcggta cggtgaaatt agcaaaatgt gcaaagtctt
    12781 ttgcgaattt agcaaaagtc tgatctagct ctgactcacc gatgcaggat aaatattttc
    12841 tgaaaatgac aatactgata gccattcctc ataattctta ctcgtaccgg aataatttac
    12901 acgtttggtt cggttttttt ggggattgaa aatatggctt tgaataagta aaagatgaaa
    12961 atatcaacgt gtcatgttga taattataaa tggattttta tgagcgtact gatatatgtt
    13021 aaagcagaaa attaaaacaa tatttgaagc attgctatat ataatgctta cttattggtt
    13081 aatagattct ttttttgctt tcaataagta tgactggatg cttgagtcgg gaggcaatat
    13141 ttgcagtata ccttccgttt caggcgaaga tcgtatattg caagcaatga ttgcagcatt
    13201 ttttcttctt acgccactta ttattcttat cttaagaaaa ctctttatgc gggaaatgtt
    13261 cgagttttgg ctatatgtat tctcgctcgt tatttgtctg gtttgtggtt ggtggctttt
    13321 ttggggacgg tttatatttt gctattagta aaatctatga gagtcgtttt aacggctctc
    13381 atagacagag aagttaccag cttaccttcg cctcaaatcc accttgttcc gcattcccaa
    13441 actcaacatt catgtcatgc agcttggcga ttcgctggac aatcgatagc ccaagtccac
    13501 tgccggtagc ggtttgtccg ggtgggcgat agaagcgttc gccaattcgc gccagtgcct
    13561 ctggtgtcac accggggccg ttatccctca cgatgaaatt atcagcattc agcgtgacgt
    13621 ctatcacgct gccctgtgga ctgtagcgca cggcgttatc cagcaaattt cgcaccaaca
    13681 aacttagcaa tagcggttgt ccggtgcgtt tgatgccgtg ggcattgagt gtcagtcgca
    13741 cgtcaatttt cgcctgctgc gccgtgtggt aaatatccat caccgacgat tgtaggagat
    13801 cttcaagcgg gatctccgcg acgtcctgaa ggttatccag tgagtccagc cgcgatagcg
    13861 tgagcagttg atcaaccaga cgagtagcgc gatcgatccc ggaatgtaat tggagcagtg
    13921 cttttttccg cgcctgcgga tcatcgtcag agagctgcgc aacttcggtt tgcactttca
    13981 gcgctgttaa cgggctacgt agttcgtgag ctgcatcgga ggtaaagcgt cgttctcgaa
    14041 ccatcatcgc atgtgtgcgg gcgaacagtt gatttagcga ctcaaccagt ggccgcactt
    14101 cgctgggtac gccagtcgcg tttagtggtt tttccgagtc agggtcacgc atacgtagtg
    14161 ccagcgccag tttgttcagc ggcgcgagtt cacgacccag tagtaccatc atgatgatta
    14221 acataatcgg cagtgcgacc agccacggga tcaattgccc ggcaacaatc gccagcgcca
    14281 tgtcttcacg gtattcccat tcctggccaa caacgatgcg atatttgcca tcaggtgagg
    14341 tcatccagac aaaacgccaa ggatcgtctt caccgaccag ttgcccgtca gcaaaacctt
    14401 cccgttgata gctataggga atatcttctc cgttatcgcc atcattaagg accattctgc
    14461 cgtcgtgggt aaagatggca aaggtcagcg catcgtcatc aacatgaccg tgttttaatt
    14521 tatttggcgt ctgtgccatg cgatccgccg cgttgatttc gttgagatcg agcgtactta
    14581 accgtttggc aaacagcatc agttgggtgt cgaacaattc atcgacgtta tccgttgttt
    14641 gtttccaggc gacaaagctg gaaagcagcc aggtcaccga ggccagaatt aaaaagatta
    14701 gcgtcagcct gacgcgcaga ctaagacgtt gggtaaattt catttctcac ctaatgtgta
    14761 gccaatacca tgcacggtac gaataaaatc actaccgagt ttgcgtcgca gatgatgcac
    14821 atgcacttca acggcattac tggtgacctc ttcgtcccag gtatacagtt tctcttcaat
    14881 cagtttgcgc ggcagtaccc gaccagcgtt acgcatcagt aattccagca gggcaaattc
    14941 ttttggtttc agtgttaagg gttcgccagc cagcgtggcg atacgtttgc cggggtcgag
    15001 catgacgtta ccgtggcgca gctcgttgct ggcctggccg ttggttcggc gcatcagagc
    15061 ctccagcctg gcggcgactt ctatcaacgc aaaaggttta cacagataat cgtcagctcc
    15121 cagacgcagc ccttctacac gttccgctaa cgcatcacgc gcggtcaggt tcagtaccgg
    15181 ctcacgctga cctttctctc gccattcgcg caaaatatcg cgaccatcca tgcctggtaa
    15241 ggttaaatcc aggatcaccg catcataagg tgcgctataa agcgcctctt ttccctgacg
    15301 accttgtgta aaccagtcga cactaaaacc cattttacta aggccagttt tgatgccgtc
    15361 accaatcagc atgtcatctt ctatcagtaa aattcgcatt ttttcatccc tgcgataacc
    15421 gtatttgctg ccagtaaacc gtgttgtaac gcgctctgta cagcattaaa ttttattttt
    15481 ttcccttaag aagttgttaa ggactatctt gttaaatgct cggaaacaga cattaaaggg
    15541 agtaataaac atgaaaaaat tcgcagcagt aatcgcagta atggccctgt gcagtgcacc
    15601 ggtgatggca gcagagcagg gcggtttttc tggcccatcg gcaacgcaaa gtcaggccgg
    15661 aggatttcag gggccgaacg gcagcgtaac gactgtagaa agcgtaaaat ccctgcgtga
    15721 cgacacctgg gtaaccctgc gcggcaatat cgttgaacgc atctctgacg atctctacgt
    15781 gtttaaagat gccagcggta ctatcaatgt tgatatcgat cacaaacgct ggaacggtgt
    15841 gacggtgacg ccgaaagata cggttgagat tcagggtgaa gtcgataaag actggaattc
    15901 tgttgaaatt gacgtcaaac agatccgcaa agtaaatccg taattgttac cgctcccggg
    15961 acgcgttccc gggaataatt tcgcagggag gcaaaatgac aaacctgaca ctggatgtaa
    16021 acattatcga tttcccatca atccctgtgg cgatgttgcc gcaccgctgt agccctgagt
    16081 tgctcaacta cagtgtggcg aaatttatca tgtggcgtaa agagacgggg ctttctcctg
    16141 ttaaccaaag ccagactttt ggcgtcgcct gggacgaccc tgccaccacc gcaccggaag
    16201 cgtttcgctt tgatatctgc ggcagcgtta gcgaaccgat tcccgataat cgttatggtg
    16261 tgagcaatgg tgaacttacc ggtggacgtt atgccgtggc ccgccacgtt ggcgagctgg
    16321 acgatatttc acacacgata tggggcatta ttcgccactg gctgcctgca agcggcgaga
    16381 aaatgcgtaa agcaccgatt ctgtttcact acaccaatct tgccgaaggg gtgacagagc
    16441 agcgactgga aacggatgtt tatgtgccgt tggcgtgatg gggaatcttt accggagtgt
    16501 gagccaaatc actcgcgata agtttgagcg aacaatgtca caggcgtatt gttgttgggc
    16561 ttataatttc tccggtaagg aataccatgt atacgcgaaa tttattatcc ggtgcgccgt
    16621 aagccgtctc gggggtatcc atgagcaagc cgcacttatg gcaggaggcg cggggcactg
    16681 gtggagacac agctaaactg ggctgaaccg tatacttaca gtcgactact ttgatgcttt
    16741 aggcttccct ggactcgcat aacctcaact tctcgaatcg cccggagcgg acccccatgc
    16801 cgagtggtgt ggcatgggcg caaccttgtg ctgccctcta tgccgatcaa gtctacagca
    16861 aagagttgta tatcaaggca cattgatgcc tttatcgccg tcggagcacg tccggcggcg
    16921 ctattcacgc tgatctcctg tgactcgacg cggcagataa tgtagtatct ccggcaatat
    16981 tgcccctttg aaggctggcg aataagttga ggaatcagaa ttaatgagcg atatggcaga
    17041 gcgccttgcg ctacatgaat ttacggaaaa cgcctactta aactactcca tgtacgtgat
    17101 catggaccgt gcgttgccgt ttattggtga tggtctgaaa cctgttcagc gccgcattgt
    17161 gtatgcgatg tctgaactgg gcctgaatgc cagagccaaa tttaaaaaat cggcccgtac
    17221 tgtcggtgac gtactgggta aataccatcc gcacggcgat atcgcctgtt atgaagcgat
    17281 ggtcctgatg gcgcagccgt tctcttaccg ttatccgctg gttgatggtc aggggaactg
    17341 gggcgcgccg gacgatccga aatcgttcgc ggcaatgcgt tacaccgaat cccggttgtc
    17401 gaaatattcc gagctgctat tgagcgagct ggggcagggg acggctgact gggtgccaaa
    17461 cttcgacggc actttgcagg agccgaaaat gctacctgcc cgtctgccaa acattttgct
    17521 taacggcacc accggtattg ccgtcggcat ggcgaccgat attccaccgc ataacctgcg
    17581 tgaagtagct caggcggcaa tcgcattaat cgaccagccg aaaaccacgc tcgatcagct
    17641 gctggatatc gtgcaggggc cggattatcc gactgaagcg gaaattatca cttcgcgcgc
    17701 cgagattcgt aaaatctacg agaacggacg tggttcagtg cgtatgcgcg cggtgtggaa
    17761 gaaagaagat ggcgcggtgg ttatcagcgc attaccgcat caggtttcag gtgcgcgcgt
    17821 actggagcaa attgctgcgc aaatgcgcaa caaaaagctg ccgatggttg acgatctgcg
    17881 cgatgaatct gaccacgaga acccgacccg tctggtgatt gtgccgcgtt ccaaccgcgt
    17941 ggatatggat caggtgatga accacctctt cgctaccacc gatctggaaa agagctatcg
    18001 cattaacctc aatatgatcg gtctggatgg tcgtccggcg gtgaaaaacc tgctggaaat
    18061 cctctccgaa tggctggtgt tccgtcgcga taccgtgcgc cgccgactga actatcgtct
    18121 ggagaaagtc ctcaagcgcc tgcatatcct ggaaggtttg ctggtggcgt ttctcaacat
    18181 cgacgaagtg attgagatca ttcgtaacga agatgaaccg aaaccggcgc tgatgtcgcg
    18241 gtttggcctt acggaaaccc aggcggaagc gatcctcgaa ctgaaactgc gtcatcttgc
    18301 caaactggaa gagatgaaga ttcgcggtga gcagagtgag ctggaaaaag agcgcgacca
    18361 gttgcagggc attttggctt ccgagcgtaa aatgaataac ctgctgaaga aagaactgca
    18421 ggcagacgcg caagcctacg gtgacgatcg tcgttcgccg ttgcaggaac gcgaagaagc
    18481 gaaagctatg agcgagcacg acatgctgcc gtctgaacct gtcaccattg tgctgtcgca
    18541 gatgggctgg gtacgcagcg ctaaaggcca tgatatcgac gcgccgggcc tgaattataa
    18601 agcgggtgat agcttcaaag cggcggtgaa aggtaagagt aaccaaccgg tagtgtttgt
    18661 tgattccacc ggtcgtagct atgccatcga cccgattacg ctgccgtcgg cgcgtggtca
    18721 gggcgaacca ctcaccggca aattaacgtt gccgcctggg gcgaccgttg accatatgct
    18781 gatggaaagc gacgatcaga aactgctgat ggcttccgat gcgggttacg gtttcgtctg
    18841 cacctttaac gatctggtgg cgcgtaaccg tgcgggtaag gctttgatca ccttaccgga
    18901 aaatgcccat gttatgccgc cggtggtgat tgaagatgct tccgatatgc tgctggcaat
    18961 cactcaggca ggccgtatgt tgatgttccc ggtaagcgat ctgccgcagc tgtcgaaggg
    19021 caaaggcaac aagattatca acattccatc ggcagaagcc gcacgtggcg aggatggtct
    19081 ggcgcaactg tacgttctgc cgccgcaaag cacgctgacc attcatgttg ggaaacgcaa
    19141 aattaaactg cgtccggaag agctacagaa agtcactggc gaacgtggac gccgcggtac
    19201 gttgatgcgc ggtttgcagc gtatcgatcg tgttgagatc gactctcctc gccgtgccag
    19261 cagcggtgat agcgaagagt aatatgccgg ggagggcgct gtttcctccc gctagcaagg
    19321 aacgccggat gaaacgttaa gtcgtcatcc ggcagcgtat tcaacccccg gaaacgataa
    19381 aaattccctt gaaaccgttg tttattcatg cgttgcgatt aacaatacgc ttttccagag
    19441 agcggctttt aacaatgccc taacctgatt tcaggtgacg tacaatgcca gtctctcaga
    19501 ccttcagagg gtgttatgct atatatcttt cgtcttatta ttaccgtgat ttacagcatc
    19561 ttagtctgtg tattcggctc catttactgc cttttcagcc cgcgtaaccc gaaacatgtg
    19621 gccacctttg ggcatatgtt tggccgtctt gcgccactgt tcggcctgaa agttgagtgc
    19681 cgcaaacctg cagatgctga aagctacggc aatgctatct atatcgctaa ccaccagaac
    19741 aactatgaca tggtgacagc atcgaacatt gtgcaaccgc cgacggtgac ggtaggtaaa
    19801 aagagcttgc tgtggatccc cttcttcggg cagttgtact ggttaaccgg caacttattg
    19861 atcgacagaa acaatcgcac taaagctcac ggcaccattg cggaagtcgt gaatcatttc
    19921 aaaaaacgcc gtatttccat ctggatgttc ccggaaggaa cccgcagccg tggtcgcggc
    19981 ctgctaccgt tcaagactgg agcatttcac gcggcaattg cggcgggcgt cccgattatt
    20041 cccgtgtgcg tctctacaac ttcgaataag attaatctta atcgactgca caacggtctg
    20101 gtgattgtcg aaatgctgcc gccaattgac gtcagtcagt atggcaaaga tcaggttcgt
    20161 gagctggctg cccattgtcg ttcgataatg gaacaaaaaa tcgccgagct cgataaagaa
    20221 gtcgcagaac gcgaagccgc cggaaaagtt taagtcggca atctgtattt ttgcggggaa
    20281 cactttcctg cacggtatta ctttagccag ttttacatgg agcaaatatg tcactcagtc
    20341 ggcgtcagtt cattcaggca tcggggattg cactttgtgc aggcgctgtt cccctgaagg
    20401 ccagcgcagc cgggcaacag caaccgctac ccgttccgcc gctacttgaa tctcgccgtg
    20461 ggcaaccgct gtttatgact gtacaacgtg cgcactggtc atttacgcca gggacacgcg
    20521 cgtcggtctg gggaatcaat ggtcgttacc tggggccgac tatccgcgtc tggaagggcg
    20581 acgatgttaa gcttatttac agcaaccgcc tgacagaaaa tgtctcaatg acggtggccg
    20641 ggctacaggt accaggcccg ctgatgggcg gtccggcacg gatgatgtcg ccaaacgctg
    20701 actgggcacc cgtactgcct attcgccaga acgcagctac tctgtggtat cacgccaata
    20761 ctcccaaccg cacggctcag caggtctata acggccttgc cggaatgtgg ctggtggaag
    20821 atgaagtcag caagtcgctg cctatcccca accattatgg tgtggatgat tttccggtca
    20881 ttatccagga taaacggctg gataactttg gtacgccaga atacaacgaa ccgggaagcg
    20941 gcggctttgt tggtgatacg ctgctggtta acggtgtaca aagcccgtac gttgaagtct
    21001 cgcgtggctg ggtgcgcttg cgactgctga acgcgtcgaa ctctcgtcgc tatcaactac
    21061 agatgagcga tggtcgcccg ttacatgtga tttctggcga tcagggattc ctgcctgctc
    21121 ctgtatcggt gaagcaactt tcgctggcac cgggcgagcg ccgcgagatt ctggtggata
    21181 tgagcaacgg cgatgaagtg tcgatcacct gtggcgaagc ggcgagcatt gttgatcgta
    21241 ttcgcggctt ctttgagcca tccagtattc tggtttctac cttggtgcta acgctgcgcc
    21301 caaccggcct tctgccgctg gtcacagaca gtcttccgat gcgcttgctg ccaactgaaa
    21361 tcatggctgg ttcgccaatt cgcagtcgcg atatcagtct gggtgatgac ccgggtatta
    21421 atggacagct gtgggacgtc aaccgtattg atgtcaccgc gcagcaagga acgtgggaac
    21481 gctggacggt acgcgcggac gagccgcaag cgttccatat tgaaggcgta atgttccaga
    21541 tccgtaacgt gaatggcgcg atgccgttcc cggaagacag aggctggaaa gataccgttt
    21601 gggttgacgg acaagtggag ctgcttgttt atttcggtca gccttcctgg gcgcacttcc
    21661 cgttctactt caacagtcag acgctggaaa tggcggaccg tggctcgatt gggcaactgt
    21721 tggtcaatcc ggtaccgtaa tcttcatact cgcccgagga gggcgagtat ggaatacctt
    21781 tcgatttctc tttcgttccg gcttataatc cccggccttt gattattttt ttatcatttc
    21841 tggaagcaaa atgagctcta tctccctgat ccaaccggat cgcgacctgt tctcctggcc
    21901 gcagtactgg gccgcctgtt ttggaccggc accgtttttg ccgatgtctc gtgaagagat
    21961 ggatcaactt ggctgggata gctgcgacat cattttggtt actggcgacg cgtatgtcga
    22021 tcacccaagc ttcgggatgg cgatttgcgg tcgtatgctg gaagcgcagg gctttcgcgt
    22081 cgggatcatc gcccagccgg actggagcag caaagacgac tttatgcgtc tgggtaaacc
    22141 gaatctgttt tttggtgtta ctgctggcaa catggattcg atgatcaacc gctataccgc
    22201 cgatcgccgt ttacgtcatg atgatgccta cacgccagat aatgtcgcgg gtaagcgacc
    22261 ggatcgcgcc acgctggttt atacccagcg ttgtaaagag gcgtggaaag atgtgccggt
    22321 gatcctcggc ggtattgagg ctagcctgcg ccgtaccgcg cattatgatt actggtccga
    22381 taccgtgcgc cgttccgttc tggtagattc gaaagccgac atgctgatgt ttggtaacgg
    22441 tgaacgtccg ctggtggaag tagcgcatcg tctggcgatg ggcgagccga ttagtgaaat
    22501 ccgcgatgtg cgtaataccg caattatcgt aaaagaggcg ctgcctggct ggagcggtgt
    22561 ggattccacc cgtcttgata ccccagggaa aatcgaccca atcccgcatc cgtatggcga
    22621 agatttgccg tgtgcggata acaaacctgt tgcgccgaaa aagcaggaag cgaaagctgt
    22681 aatcgtgcag ccaccgcgcc agaaaccgtg ggaaaaaacc tacgtattgc tgccttcttt
    22741 cgagaaagtg aagggcgata aagtgctgta cgcccatgct tcgcgcattc tgcaccacga
    22801 aaccaaccca ggctgcgccc gcgcattgat gcaaaaacac ggtgaccgct atgtgtggat
    22861 caacccgcct gctatcccgc tttctaccga agagatggac tgcgtttttg cgctgccgta
    22921 caagcgcgtg ccacatccgg cctatggcaa tgcccgtatt ccggcttacg aaatgatccg
    22981 tttttcggtc aacattatgc gcggctgctt tggcggctgt tctttctgtt ctattaccga
    23041 gcacgaaggg cgcattattc agagccgttc ggaagattcg atcattaatg agatcgaagc
    23101 gatccgcgac accgttccag gttttacggg cgtgatttcc gatctcggtg ggccaactgc
    23161 caacatgtat atgttgcgct gcaaatcgcc acgcgctgaa caaacttgtc gccgtttgtc
    23221 gtgcgtttat ccggatattt gtccgcacat ggacactaac cacgaaccga cgatcaacct
    23281 ctatcgccgt gcgcgtgatc tgaaaggcat taaaaaaatc cttatcgcct caggcgtacg
    23341 ttatgacatc gccgtggaag atccgcgcta tatcaaagag ttggcgaccc atcacgtcgg
    23401 cggttatctg aagattgctc cggaacatac cgaagaaggg ccgttgtcga agatgatgaa
    23461 gccgggcatg ggcagctatg accgctttaa agagctgttc gatacctact cgaaacaggc
    23521 aggtaaagag cagtatctga tcccgtattt catctctgcg caccccggta cgcgtgatga
    23581 agatatggtg aatttggcgc tgtggctgaa aaagcatcgt ttccgtctcg accaggtaca
    23641 gaacttctac ccatcgccgc tggcgaactc aaccaccatg tattacaccg ggaaaaaacc
    23701 gctggcgaag attggttata agagcgaaga cgtcttcgta ccgaagggcg acaaacagcg
    23761 tcgtttgcat aaagcgttgt tgcgttacca cgatccggca aactggccgt taatccgcca
    23821 ggcgctggaa gcgatgggca aaaagcatct gattggcagc ccgcgattgc ttagtgcctg
    23881 cgccaaccat tgaagagatg cgtgaagctc gtcgccagaa ccgcaatacc cgtccggcgt
    23941 tgactaaaca tacgccgatg gcgactcagc gtcagacgcc tgctacggca aaaaaagcgt
    24001 cgtctacgca atctcgtccg gtgaatgctg gtgcgaagaa acgccctaaa gcggcggttg
    24061 gacgttaaaa aaaatccccg gcaactgaca tgctgccggg gattttttta tcattctgcg
    24121 tacatacgca agcagcgcag gcaatcaaaa tagtcctgtg catcaagacg cgtattggct
    24181 ctgttagctt cggatttggc agcatagatt tgtgggcgta gtgctttcat atcctgctca
    24241 agccgggcga ttttggcatt taatgtttgt acattgctcg ccagttgatt gaccttttgc
    24301 tcattgacgc agccactgag caagcaggtt gccagaacaa cagctcccac ggtgaaaatc
    24361 gttttcatat agtgttccct tttatttatt gttccggcta attgttattt aattcagctc
    24421 attgctttaa tctggcggcg gtttttgcct caatcagctt aagtaggctg ccgctgttcg
    24481 ggcggataaa atcagcgatt tcccacttac cttcgcgccg ctgtaaaacc agctcttgct
    24541 cttcattgtt gccattggtc agcgtaaaac gaacatgaat gcgggcggaa tcggcggttt
    24601 tctccaggac tcgaatttca tcaacgcgtg aaaatagcga ctcttcgcca aaacagatcg
    24661 gattgtcgta aataaattgc cgttctattt gcatatcttc atcgggatta ttggccgtct
    24721 tgaaggtggt tttatagaag gcattaaccg cctggcggta agagtcggaa accacccacg
    24781 tccacggcca gctataatca gcaatatgtt caaagagttc agcaacaaag gattctggct
    24841 gttctttctg caacgaagct aaagcggcca gcgttttttc attctcgcta ttaactgctt
    24901 gtaagacgct gccatgattg ctgacaatat catcaatgac ccaacgacca ttttccgcca
    24961 ccattttcag tgtctgtgtg gtcttttctt tatcatcttt aaagatacga aagcgcacaa
    25021 cggcatcggc atgatcgacg tcagtttggg ttatcgctac gctttctaac accagatcgc
    25081 caaaatcctg acaatcacaa accggatcat aatccagcca gccaatattg cccggtagtg
    25141 taagattgtt gttcagggta agcgcctgtt gaatacgcgc tgaagttatc gcccgctctc
    25201 cggtttcacc gaaataaggc gtactggcat ctgatttata gttctgataa atctgccgga
    25261 ctgtttgttc tacggtctgt gaaggaggct gcgcgtgtac ggtaaaactt gccagggctg
    25321 ctaaaaacag aagtattatt ttcatctgtt tttcacctct ccgttgcgtt tatcccggat
    25381 atagatgagt tgttctttta agagcttccg gctctgcatg atgatgtcct tatatttggc
    25441 attcctgaac aaggaagagt aacaacgggc gggacgcgag gggaataaat gatttctgaa
    25501 aagtccggta gcggaacatt accgccaccg ggagaatttg cacgttagcc gccgaactgg
    25561 tcaggatcgg gaccgagacg cttgccctga tcgagttttg caattttgcc gagttcgtct
    25621 ttgtcgagac ggaaatccca gacatcaaag ttttcggcaa tacgtgaagg tgtgaccgat
    25681 ttcgggatca ccaccaggcc gctatccaga tgccagcgga taacaatctg cgccggggtt
    25741 ttgccgtatt tatctgccag atcgcgaatg actttctgat cgaaaacgcc ttttcctcct
    25801 tgcgctaatg ggctccagga ttcggtctgg attttgtgtg tcgcgttcca ggcgtgtagc
    25861 tggcgttgtt gcatcagcgg atgaagttcg atctggttta tcacaggcgt cacgccagtt
    25921 tcatcaatca ggcgttgcag gtgatggatc tggaagttgc acacgccgat gcttttgatt
    25981 aatccctctt tttgcaattc gatcatgcct ttccatgctt cgacataatg gtcgatagcg
    26041 ggaacgggcc agtgcattaa gtagaggtcg atataatcaa gctggagttt tttcaggctg
    26101 tcgagcaggg cttcgcgggg gcacttgtgg tcgtcgttcc acagcttagt ggtgatgaac
    26161 agttcttctc tgttgactga ggcatttttc agggctttgc cgacaccttc ttcgttcttg
    26221 taggccgcgg cggtatcaat cgagcgataa cccacttcta acgctttttg aatggcggtg
    26281 attacttcct cattacttgc ttgccagacg cccagtccca gctggggcat gacattgcca
    26341 tcctgtagct taataacggt tggattagcc atacgttcct cctttatatg aactcaccgg
    26401 agcaggctcc ggtgaggtgt tgtgaactta agtctggacg aaatgcccga aaacgaaagt
    26461 ttgaggcgta aaaagcttag cgggcggctt tgtatatacg gcggctgaca tccaacgtaa
    26521 tgtcatgatt ttcgcccagt tgggtcatgc cgtgctcttc cagttttttc agcaaagccg
    26581 ggatggagct gccgtccaga ccgtagtcgg agaggtgggt cggcacgcct aattgctcaa
    26641 agaaattgcg ggttgcggca atcgcggcgt caatacgctc atcgtcggaa ccttcagtga
    26701 tgttccagac gcgttcagca tattgcagca gcttagcgcg cttggtctcg cgtttttcat
    26761 tccacagtgc aggcaggacg atagccagtg tttgcgcgtg atccagaccg tgcatcgcag
    26821 tcagttcgtg gcccagcata tgcgttgccc agtcctgcgg tacgccagcg ccaatcaaac
    26881 cgttcagcgc ctgagtcgcc gcccacatga cgttggcgcg cacatcgtag ttttctggct
    26941 ctttcagggc tttcggacca tcttcgatta gcgtcagcaa aatgccttct gcgaaacggt
    27001 cctgaatttt ggcatcaacc ggtttggtaa catactgttc cacggtgtgt acaaaggcgt
    27061 ccactacgcc gttagccacc tgacgcggcg gcagggtgta ggtataaacc ggatcgagca
    27121 cggcaaatac cggctgaaca tgtgcagaat ggaacgcctg cttgtcgcct gtggttttac
    27181 gggagatcac cgcgcctgcg ttggattctg aaccggttgc tggcagcgtc agcacacagc
    27241 ccatcgggat ggcgctttta atctctttac cgcccgtttg cagaatgtgc cacggatcga
    27301 tattttccgg atagttagcc gctgcggcga taaatttggt gccgtccagt acagaaccgc
    27361 cgccaaccgc cagcaggaaa gtcactttct gttcgcgaac cagtttcacg gcgttcatca
    27421 gcgtttcata agccgggttt ggctcaatac cgccaaattc cagcacgtcc atgcctttca
    27481 gggcatccag aacttgatcg agaacgccgg tttttttcac gctgccgcca ccgtaggtaa
    27541 tcaatacgcg agcatcgtga ggaatttgtt cgcgtaaacc agcgattgcg cctttaccaa
    27601 acagaatgcg ggttggggtg tgcagattaa agttgttcat tacttgctcc ctttgctggg
    27661 ccaatatgag ggcagagaac gatctgcctg atgtttttca ttgtgatcgc cagcgcccta
    27721 gctctcaatg ctcatttctg ccaatgtctt gcctatttct ccagagtgct ggagaaatgc
    27781 tacaaaattg cgcacaatca aattgccgca ttattcctaa gaaattacgc gatatgaaac
    27841 gtgaagagat ttgtcgcttg ctggcggata aagttaataa actgaaaaat aaagaaaata
    27901 gtttgtcaga actgttgccc gatgtgcgtt tgttgtatgg cgagacgcct ttcgcacgta
    27961 caccggtgat gtacgagcct ggcatcataa ttctcttttc cgggcataaa atcggttata
    28021 tcaat
//
LOCUS       DADMGC010000042        27738 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.042, whole genome shotgun sequence.
ACCESSION   DADMGC010000042 DADMGC010000000
VERSION     DADMGC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.042"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..1100)
                     /locus_tag="JNZ73_001789"
     CDS             complement(<1..1100)
                     /locus_tag="JNZ73_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAY8732093.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGA"
     gene            complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="JNZ73_001790"
     CDS             complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="JNZ73_001790"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HAY8732094.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="JNZ73_001791"
     CDS             complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="JNZ73_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HAY8732095.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="JNZ73_001792"
     CDS             complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="JNZ73_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HAY8732096.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="JNZ73_001793"
     CDS             complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="JNZ73_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HAY8732097.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="JNZ73_001794"
     CDS             complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="JNZ73_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HAY8732098.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3404..4381
                     /gene="pspF"
                     /locus_tag="JNZ73_001795"
     CDS             3404..4381
                     /gene="pspF"
                     /locus_tag="JNZ73_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HAY8732099.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCATLNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="JNZ73_001796"
     CDS             complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="JNZ73_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HAY8732100.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHSDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5804..7084)
                     /locus_tag="JNZ73_001797"
     CDS             complement(5804..7084)
                     /locus_tag="JNZ73_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY8732101.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7086..8574)
                     /gene="puuC"
                     /locus_tag="JNZ73_001798"
                     /pseudo
     CDS             complement(7086..8574)
                     /gene="puuC"
                     /locus_tag="JNZ73_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="JNZ73_001799"
     CDS             complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="JNZ73_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HAY8732102.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNVN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="JNZ73_001800"
     CDS             complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="JNZ73_001800"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HAY8732103.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10284..11702
                     /locus_tag="JNZ73_001801"
     CDS             10284..11702
                     /locus_tag="JNZ73_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HAY8732104.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11851..12015
                     /locus_tag="JNZ73_001802"
     CDS             11851..12015
                     /locus_tag="JNZ73_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732105.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12005..13390
                     /gene="puuP"
                     /locus_tag="JNZ73_001803"
     CDS             12005..13390
                     /gene="puuP"
                     /locus_tag="JNZ73_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HAY8732106.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13524..13769
                     /locus_tag="JNZ73_001804"
     CDS             13524..13769
                     /locus_tag="JNZ73_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HAY8732107.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14082..15725
                     /gene="sapA"
                     /locus_tag="JNZ73_001805"
     CDS             14082..15725
                     /gene="sapA"
                     /locus_tag="JNZ73_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HAY8732108.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVDVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15722..16687
                     /gene="sapB"
                     /locus_tag="JNZ73_001806"
     CDS             15722..16687
                     /gene="sapB"
                     /locus_tag="JNZ73_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HAY8732109.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16674..17564
                     /gene="sapC"
                     /locus_tag="JNZ73_001807"
     CDS             16674..17564
                     /gene="sapC"
                     /locus_tag="JNZ73_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HAY8732110.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17564..18556
                     /gene="sapD"
                     /locus_tag="JNZ73_001808"
     CDS             17564..18556
                     /gene="sapD"
                     /locus_tag="JNZ73_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HAY8732111.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18558..19364
                     /gene="sapF"
                     /locus_tag="JNZ73_001809"
     CDS             18558..19364
                     /gene="sapF"
                     /locus_tag="JNZ73_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HAY8732112.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19432..19785
                     /locus_tag="JNZ73_001810"
     CDS             19432..19785
                     /locus_tag="JNZ73_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HAY8732113.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20153..20941
                     /gene="fabI"
                     /locus_tag="JNZ73_001811"
     CDS             20153..20941
                     /gene="fabI"
                     /locus_tag="JNZ73_001811"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAY8732114.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21087..22214
                     /locus_tag="JNZ73_001812"
     CDS             21087..22214
                     /locus_tag="JNZ73_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HAY8732115.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRENNHRLAQITQYARQLASSPAIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22282..24216
                     /locus_tag="JNZ73_001813"
     CDS             22282..24216
                     /locus_tag="JNZ73_001813"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HAY8732116.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24294..24396)
                     /locus_tag="JNZ73_001814"
                     /pseudo
     CDS             complement(24294..24396)
                     /locus_tag="JNZ73_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24450..26424
                     /gene="pdeR"
                     /locus_tag="JNZ73_001815"
                     /pseudo
     CDS             24450..26424
                     /gene="pdeR"
                     /locus_tag="JNZ73_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26572..26745
                     /locus_tag="JNZ73_001816"
     CDS             26572..26745
                     /locus_tag="JNZ73_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732117.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26835..27584
                     /locus_tag="JNZ73_001817"
     CDS             26835..27584
                     /locus_tag="JNZ73_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY8732118.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT         6433 a         7194 c         7124 g         6987 t
ORIGIN      
        1 aatgcacctt cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag
       61 ccacgcagtg ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt
      121 ttactggagg gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt
      181 tgcaccgcat gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca
      241 tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaatgatc
      301 actgtacctg gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt
      361 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
      421 cggacatact cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac
      481 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
      541 cgcgtggaat tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggacgggtg
      601 acggcactga gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc
      661 tgttgtaaat agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca
      721 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
      781 gcgacctgat gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt
      841 aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg
      901 ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca
      961 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
     1021 gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccaggtaa
     1081 tccgtaattt tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact
     1141 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
     1201 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata
     1261 aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt
     1321 gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat
     1381 ctcgctaagg atctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac
     1441 ggtgtcgttt ttatccggaa ctgcggtggc aatgcgctct ttcacttcct tcaggggaat
     1501 attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca
     1561 gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt
     1621 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
     1681 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
     1741 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
     1801 ttttatcgcc cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
     1861 cttgcctgct aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt
     1921 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
     1981 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
     2041 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
     2101 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaagaaaatc
     2161 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg
     2221 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataatttttt attgagatta
     2281 atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg
     2341 cctgaatacg ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct
     2401 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
     2461 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
     2521 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
     2581 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
     2641 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
     2701 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
     2761 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
     2821 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt
     2881 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag
     2941 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
     3001 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
     3061 gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag
     3121 tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc
     3181 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
     3241 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
     3301 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
     3361 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga
     3421 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
     3481 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc
     3541 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
     3601 gacgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
     3661 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
     3721 tgatgaactc gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta
     3781 cggtgaactg gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg
     3841 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
     3901 cgaccggctg gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat
     3961 tatgctgatg gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt
     4021 cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat
     4081 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
     4141 gcttgatgac atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt
     4201 ttcagaaaat acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca
     4261 ggaaaaagag ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc
     4321 tgaattactg gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
     4381 gcgaagttga gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa
     4441 tcaatacgtc gtttgcaggc ctaataagcg cagcgcatca gacattattg cgttgggcta
     4501 ttaatcgctc agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat
     4561 ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag
     4621 cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt
     4681 ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc
     4741 cggaacgctt tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc
     4801 gcgttcgcag agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc
     4861 cagcgggttc ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt
     4921 cgcgttaccg accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat
     4981 taagtccggc ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc
     5041 gctttgcact tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc
     5101 ggcaaccagc tcttttggcg caacgttgaa accgccctcg ccctgcaccg gttcgaaaat
     5161 aatggccgcg acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag
     5221 ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc
     5281 agggaacggg ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat
     5341 atatgtacga ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc
     5401 gcgagcaatt ttcaccgcgt tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt
     5461 agcctgcccg ctcactgggg caagggcgtt aattttctcc gccagcgtga cgtagctttc
     5521 gtacggcaca atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc
     5581 gaccagatca gagtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat
     5641 gtattcgttg ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt
     5701 acacatcacg ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt
     5761 gctcatgttt cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat
     5821 cgcgcaggct gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc
     5881 cggggaacgg gtaatgcggc agattggcga aggcatcgaa acgctcggcg tcaccgcgca
     5941 gcagttcagc aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac
     6001 cctgcatgta atagatgttg gtatcaaggc ggccgaactg cggcatgcgc gacaaggtca
     6061 gcaggaagtt gcccgtccag cggtaatcaa ttttcacgcc cttcaactga gggaaggttt
     6121 ttagtagttt cggtaccaca agacgctcaa cgtcatccgg gttgcgtgca ccatacacta
     6181 cacccccgcc gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt
     6241 tacagtcttc cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc
     6301 gttcggtggt gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta
     6361 ctttatcgcc cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat
     6421 tggcagtccg aaccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat
     6481 acacgcgccc gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt
     6541 gaatatgccc accgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc
     6601 ggcgaatagc gttggcgtcc agcagctcca gctgtttatt gccgtagcgt tcccagttct
     6661 ctttctgctc ttcgagtgtg gcgagttgtt tatcattcat cgccacaaac agaccgccgg
     6721 ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccaccct
     6781 cgaacatcat gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt
     6841 cgatgtcgcg gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc
     6901 cgatgcgtga ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg
     6961 aggagagccc ggtatagccg ccgccaacca cgcaaacgtc gcaggtgatt gactcattca
     7021 gtgtatcgaa tggtgcatat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg
     7081 tcatttcagg cctccaggct tatccagatg gttttcagtt cagtgaattt ttcaagggca
     7141 tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata
     7201 tcgccgtcgt tgtagttatt gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg
     7261 tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg
     7321 gcaagctgca gcgcctgatc ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa
     7381 atctcttcgc ggcttaagga cgcattcggg tccacatcca cgaagatggt cgggccgatg
     7441 gccgctgcca gcccggcgtt gcgaccatcc agcagcagtt gccccttgcc ttcgccttca
     7501 cgaataaagg tgtgtaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt
     7561 gcgggatcaa gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag
     7621 aattcatcgg cgatgctctc ttccagtaac aaacgcgttc cggcgatgca cacctggccc
     7681 tggttgtaga agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg
     7741 gcgaaaacga tgttggcgct tttgccgccc gcttccagcc agacacgttt catgttgcta
     7801 tcacccgcgt ctttcagcag ttgtttaccc gtacgcgtgg agccggtaaa ggcgatggca
     7861 tcgatatcgt tatgacgcga aagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg
     7921 ttcaacacac catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg
     7981 agcggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag cgccgggccg
     8041 agtttccagc aagtcagcaa cagcgggaag ttccacggca cgatggcggc aatcacgccg
     8101 accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac
     8161 actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca
     8221 cgcagactgt gacgaatccg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc
     8281 ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga
     8341 agagagtgac cagtcgccgc gttcaaatac gccgcgagct gcgctcaccg cacggtcgat
     8401 atcgacgctc ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt
     8461 ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc
     8521 aatggcgagg cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac
     8581 tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg
     8641 ggctgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt
     8701 acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg
     8761 caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt
     8821 tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt
     8881 actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg
     8941 tacgtttcaa agatcatcgc cagcgtgcga ttcgggttaa cgttatgaac cagcttcatt
     9001 gacaccccct gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca
     9061 tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc
     9121 ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg
     9181 tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt
     9241 tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact
     9301 gtagtggtca gagtcgctgt ttttcagcga tatggtgctg ccaagcggtg ataaagccct
     9361 cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc
     9421 ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag
     9481 cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc
     9541 aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt
     9601 catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga tgctccagca
     9661 attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt
     9721 gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg
     9781 ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata
     9841 ggtgcggctg cacattgctg ggactactag gaagataaat gccatcgagt ttcggcaaaa
     9901 gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg
     9961 cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc
    10021 tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc
    10081 tcacaaagtg gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat
    10141 tttttcaatc tagcagtgga ttttcatttt tgcaaaccaa atttaacatt tgacaaacat
    10201 ttggtttgca tacagattcg aatgatggtc attatatttt acgctttgat aacgagcgga
    10261 aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc
    10321 agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc
    10381 gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg
    10441 gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct
    10501 cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa
    10561 tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct tctgccgccg
    10621 atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg
    10681 acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt
    10741 tcccggttgt agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag
    10801 gggatctgca accgccctgc gcgcctggca ccgatgaccg caatacacaa agccaggttt
    10861 actccgttga taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc
    10921 agttgcagct gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa
    10981 tcaaccttta ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac
    11041 gcctggtgcg tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt
    11101 atgaagagca cgcgggcagc gggatgcata tccatatcag tatgcaaaat aatcgtggcg
    11161 agaatgtgct ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg
    11221 ggatgattga cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc
    11281 gccgcttcca gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca
    11341 ccgtcgccct gcgtattccg tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg
    11401 cgggtgccga tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg
    11461 gccttgataa cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag
    11521 gcttaccctt cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc
    11581 tgcgccgcta tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc
    11641 tgttgcagtt tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt
    11701 aattcccctt gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt
    11761 aatccagaca tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa
    11821 cgattaagac tgtgacaagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag
    11881 ggaggctgcg tatgcgcccc tcctgctact gactggactc cgcccccttt gcttcccttg
    11941 ctaaacaggt ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc
    12001 aattatggct attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg
    12061 aaaatcactg aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac
    12121 cgtatttgat acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta
    12181 tttgctggcg ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca
    12241 gtttccggag gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg
    12301 atttatggtc ggctggtcat cactgctgga ttatctcttt ttgccaatga tcaacgtttt
    12361 gttggcgaaa atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt
    12421 aaccttcgtc gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt
    12481 caataccctg tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt
    12541 tcagggactg cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag
    12601 cgagaacgcg cacctgatcc cgattattac cggtgcgacg attgtctgtt tctcgttcct
    12661 cggtttcgat gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc
    12721 gaaagccatc ttcctgacgg cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt
    12781 tatgcagctg ttctttcccg atatcagccg ctttaaagac ccggacgccg cactgcctga
    12841 aattgcgctg tacgtcggcg gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt
    12901 gaacacatta gcgtctggcc tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat
    12961 ggggcgtgac aatgtgtttc cagagcgcgt gtttggctat gtgcacccaa aatggcggac
    13021 tccggcactc aacgtcatta tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt
    13081 agtcaccgcg acagcactga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct
    13141 gtcggtgttt aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt
    13201 ccactatttg ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct
    13261 agagtcaacg tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg
    13321 gtacctgatc cgccgctatc gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag
    13381 cgaaacgtaa tagccggatg cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca
    13441 ttaactataa aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag
    13501 cgcattaacc ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg
    13561 ccgatgagta cgcgaccgaa tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg
    13621 cccactactt ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag
    13681 cccaacatga aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag
    13741 agtttttgaa tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg
    13801 ttgtccggat aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct
    13861 ttaatgggtt tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata
    13921 gttatagaga aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac
    13981 caaccacacg gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa
    14041 tattagtaag atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt
    14101 ctcttttggt gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc
    14161 cgcatgctga tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct
    14221 ttaacccatc caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg
    14281 atcgactgct ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct
    14341 gggaagtact cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc
    14401 aaaaaaccga ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct
    14461 tccagcgaat ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct
    14521 acttcgacag cctgcaattt gccgataacg tgaaaagcgt ccgcaaactg gataatcata
    14581 ccgttgagtt ccgactcgcc cagccggatg cttctttttt gtggcacctc gcaacccatt
    14641 atgcttcggt catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc
    14701 aactcgaccg tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc
    14761 aatttattcg cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg
    14821 tagtggtgga tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat
    14881 gcgacgttct ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct
    14941 tgcgtttaac gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac
    15001 cgccgctaaa taatcccgct gtccgccatg cactggcact ggcgattaat aaccagcgcc
    15061 tgatgcaatc catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct
    15121 cgtgggccta tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac
    15181 agttgaaggc gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc
    15241 aggcgtggaa ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg
    15301 ttgacgtaaa agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg
    15361 atatgagcca tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt
    15421 tcttccgtcc gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt
    15481 gcgatccgaa attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc
    15541 gtattgaagc ctatgacgaa gcgcagagta ttctggcgca ggaattacct attctgccgc
    15601 tggcgtcgtc attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc
    15661 cgtttggtaa cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac
    15721 catgattatc ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac
    15781 cttcgttggc ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact
    15841 gtggaatgcc tgggtgttct ggtttaatgg cctgatccac tgggattttg gtgtttccag
    15901 cattaacggt caaccaattg ccgaacagtt aaaagaggtc ttcccggcga cgatagaact
    15961 gtgcatcctc gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg
    16021 gattacgcgc cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc
    16081 aatcccggtg ttctggctgg cgcttctgtt gacactgttt tgttcactca cgctaggttg
    16141 gttgcccgtt tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc
    16201 gttgattgat gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat
    16261 ccgccatatg atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg
    16321 cctgatgcgt atcagcacta ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac
    16381 ccgcggtttg tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc
    16441 ggttattcct cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga
    16501 aatggttttt agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga
    16561 ttacgcagcc atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt
    16621 gatttctgat attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt
    16681 acgatagcgt atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat
    16741 tttatagtga cgcctctgcg atggtcggcc tgtacggctg cgcgggactg gctgtactgt
    16801 gtatttttgg cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttatcaat
    16861 tattgccgcc atcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc
    16921 tcggacgtga tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat
    16981 ttgtggtgac gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga
    17041 cacacggcct ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt
    17101 cgctgctgtt ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt
    17161 ttgctgtctg gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg
    17221 acgaactgga aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata
    17281 ttctctggtt tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg
    17341 cactgtcgat ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac
    17401 tcccctcgcc tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc
    17461 cgtggactgt catgctgcca ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc
    17521 ttggcgatgg cgttcgtcgt gcgattattg cgggggtgga ataatgccat tactcgatat
    17581 tcgtaacctg accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt
    17641 aagtatgacc ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa
    17701 gagtttgatt gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga
    17761 ccgtatgcgt tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact
    17821 ggttggccat aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga
    17881 acgtgtgggt cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg
    17941 gcagcgtttt ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa
    18001 agatcacaaa gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa
    18061 agtgatgata gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac
    18121 aaactcaatg gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca
    18181 aaacagcaat accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc
    18241 ggataaaatt aacgtgcttt actgcggtca aacagtggaa accgcgccga gtaaggagtt
    18301 ggtgacgatg ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg
    18361 cagcgcgatg ccgcataaaa gtcgcctcaa tactctaccc ggcgctatcc cattgctgga
    18421 acagttaccg attggttgtc gtctggggcc acgttgcccg tatgcacaac gagaatgcat
    18481 tgtgacgcca cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa
    18541 catggagaaa gagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt
    18601 tccgctaccg gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct
    18661 ttacgctacg tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca
    18721 cactggcaaa aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg
    18781 atcatccgct gcattttggc gattattcct tccgtagtca gcgtattcgc atgatttttc
    18841 aggatccttc gacctcgttg aatccccgtc aacgtatctc acaaattctg gattttccac
    18901 tgcgcctgaa caccgacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc
    18961 gtatggttgg gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccggac
    19021 aaaaacagcg tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg
    19081 atgaagccct cgcttcactg gatatgtcga tgcgttcgca gttgattaat ctgatgctgg
    19141 agttacagga aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga
    19201 agcacattag cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca
    19261 ccgcggatgt gctggcatcg ccgctgcatg aactcaccaa acggctgatt gccgggcact
    19321 ttggcgaagc attgacagcg gatgcgtggc gtaaagatcg ctaattttct gaatcctcct
    19381 tccagattat tcacctcatt ctgacaagag attaaatctt gtcagaatga ggtgatggat
    19441 aaaataaaat caaatgcgcg tgatttaaga cgcaatctca ctttgcagga acgaaagctc
    19501 tggcgatatc ttcgcagccg acgttttggt gatttcaaat ttcgccgtca acatccggtg
    19561 gggagctaca ttctcgattt tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt
    19621 gggcagcatg atttggcagt tgcctatgat accaggcgca ctagctggct tgagtcgcag
    19681 ggctggaccg tgctgcgttt ctggaataac gagattgatt gtaatgagga ggcggtgctg
    19741 gagattattc tgcaggaact gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc
    19801 gaccgagccg aatagctgtt gtggtgaaaa catggagaca gtgctggaga atattcggca
    19861 aggtctgaac catcccagcc atcgccataa aagggttagg ggctgtatga gcctgtttgt
    19921 tgctggggta acaatatttg cacaatatgg tcccctcgcc cctctgggga gagggttagg
    19981 gtaaggggaa aagcgccccc cctgccgcag cctgctccgg tcggacctgg caactatagc
    20041 tactcacagc caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga
    20101 cgaatcgcct gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt
    20161 tctttccggt aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg
    20221 tatcgctcag gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa
    20281 actgaaaggc cgcgtagaag aatttgccgc tcaattgggt tctgacatcg ttctgcagtg
    20341 cgatgttgca gaagatgcca gcatcgacac catgttcgct gaactgggga aagtttggcc
    20401 gaaatttgac ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga
    20461 ctatgttaac gccgttaccc gtgaaggctt caaaattgcc cacgacatca gctcctacag
    20521 cttcgttgca atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac
    20581 cctttcctac cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa
    20641 agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg
    20701 tgttaacgcc atctctgctg gtccgatccg tactctggcg gcctccggta tcaaagactt
    20761 ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt cgccgtaccg ttactattga
    20821 agatgtgggt aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga
    20881 agtagtccac gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata
    20941 atcgttctgt tggtaaagat gggcggcgtt ctgccgcccg cgttatctct gttatacctt
    21001 tctgatattt gttatcgccg atccgtcttt ctccccttcc cgccttgcgt caggataacg
    21061 atttccttta cgaccaagga gcgcccatgg aacaacgcca catcaccggc aaaagccact
    21121 ggtatcatga aacgcaatcc agtactacgg agtatgacgt tctgcctctg gtcccggaag
    21181 ccgcaaaggt cagcgatccc tttctgctcg acgtgatcct tgatgaagag acgctggccc
    21241 ccttcctttc atggctggtc cctgcgcgcg ttcttgcagt ggagttgttc cctgaccagc
    21301 ttaccgtgac ccgttcacaa accttcaccg cttatgaacg cttgtcgacg gccctgacgg
    21361 ttgctcaggt ttgcggcgtc cagcggttat gtaactacta ttcggcgcga cttacgccgc
    21421 tccccggacc tgattcctcc agggaaaata atcatcggtt ggcacaaatc acgcaatatg
    21481 cccgccaact ggctagctcg cctgctatta tcgacaaccg atcgcgccag catctgaatg
    21541 acgtcggtct tactgtctgg gactgtgtga tcattaacca aatcattggt tttattggct
    21601 ttcaggcgcg gacaattgcg acatttcagg cttatctcgg gcatccggta cgctggttac
    21661 ccgggctggc gatacaaaac tacgccgacg cgtcactgtt tgctgatgaa tcaatacgct
    21721 ggcgaagcag ctatgaggtg gaaaaactac ctgaagagca cacaaaaagt tcaactgcag
    21781 aactttgcca actggccgaa acacactctc tccaccctat ttcactttcc cttctcgaaa
    21841 agttgttaaa cagcacacgg gtcaatacac agccggataa tcagcttgcg gcgttgttat
    21901 gcgcgcgtat aaatggcagt cctgcttgtt tttccacctg tatggattca tcaaatgaat
    21961 ataaaaaaat cagcaccctt ctgcgcaagg gcgaaaatga aattaaccga tgggctgacc
    22021 gtcattctgt tgagcgcgct accgttcagg cgatacaatg gctgacccga gcacccgatc
    22081 gctttagcgc cgcccagttc agccctttac tcgaacacga aaaatcatca acgcagatta
    22141 ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa atcgcgttgg
    22201 gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca gccgctctaa
    22261 tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca gcttaaacag
    22321 caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga aaaaggcttt
    22381 ggcttcctgg aagtcgacgc acaaaaaagt tatttcattc cgccgccgca gatgaaaaaa
    22441 gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg tgaatccgca
    22501 gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt tcagggcaaa
    22561 aatgaccgtc tgaccatcgt tcctgatcat ccacttttaa aagacgccat tccttgccgc
    22621 gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc cgaaatgcgc
    22681 cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata catcactttt
    22741 ggcgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct ggaaaaagaa
    22801 gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga agatctgacc
    22861 tcgctggatt ttgtcaccat cgacagtgcc agcactgaag atatggatga cgcccttttc
    22921 gctaaggcgc tgccggatga caaacttcag ctgattgtgg cgattgccga tccaaccgcg
    22981 tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt caccaactat
    23041 ctgcctggct tcaacatccc tatgctgcct cgcgagcttt ctgacgatct ctgctcactg
    23101 cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc tgatggcacc
    23161 attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa gctggtgtat
    23221 gaccaggttt ctgactggct ggaaaatacc ggtgactgga agcctgaaag tgaagcaatt
    23281 gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg gcgtcataac
    23341 cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga aaaaggtgaa
    23401 gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga agaagcgatg
    23461 attgccgcta acatttgtgc ggcccgcgta ctgcgcgata agctcggttt tggcatctat
    23521 aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt gctgaaaacg
    23581 cacggtctgc atgtcgatgc cgaagaagta ctcacgctgg acggtttctg caaactgcgt
    23641 cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg cttccagtca
    23701 tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga agcatacgcc
    23761 acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct gctgaaagcg
    23821 gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca aatggccgaa
    23881 cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata cgcacgcttc
    23941 ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga tatcagccgt
    24001 ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc ggcacctttc
    24061 ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt acaaattaaa
    24121 ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga agtccgcatg
    24181 gaaacccgca gcattattgc gcgcccggtc gcgtaatctc ctttcacggc ccattcctca
    24241 tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat tttttaactg
    24301 actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata tataaaaaac
    24361 aatgcgttta tccgccgtta atttcatgta agtcatgttg ttccttttat ttaaccgcgg
    24421 actccgctgt taaccggagg atatgcatca tgaaaaccgt tagggagtcc acaacgttgt
    24481 acaactttct cggatcgcac aatccatact ggcggttgac ggaaagcagc gatgctttgc
    24541 gcttttctac caccaaaacc acagaacctg atcgtacatt gcagttatct gccgaacagg
    24601 ctgctcgcat cagggaaatg acggtcatca cctccagcct gatgatgagt ctgaccgtcg
    24661 atgaaagcga tctttctgtg catctggtag gacgaaaaat caataaacgg gaatgggctg
    24721 gcaacgcgtc tgcatggcat gacacaccgg cggttgctcg tgatttatca cacgggcttt
    24781 cctttgctga gcaggtagtt tctgaagcac attccgcaat agtgattctc gacagccggg
    24841 ggaatatcca acgcttcaat cggttatgtg aagattacac agggttgaaa gaacacgacg
    24901 tcattgggca aagcgtgttt aaactgttta tgagccgtcg tgaagctgcg gcatccaggc
    24961 gcaataaccg tgtatttttt cgaagcggca atgcatatga agtcgaactg tggataccaa
    25021 cacgtaaagg ccagcggctg tttctgtttc gcaataaatt tgtccacagc ggcagtggca
    25081 aaaacgagat ttttttaatc tgttccggta ccgacattac cgaagagcgc cgcactcagg
    25141 agcgactgcg tattctggca aataccgaca gtatcaccgg actgccgaat cgtaacgcaa
    25201 tgcaggagtt aatcgatcac gctattaatc aggcagataa caataaagtt ggggttgtgt
    25261 atcttgattt ggataatttt aaaaaggtca acgacgccta tgggcatttg tttggtgacc
    25321 agttattacg cgacgtgtca ttggctattt taagctgtct cgaacatgac caggtgttgg
    25381 cgcgtccagg tggcgatgag tttctggtac tggcatccaa tacctcacaa agcgcgctgg
    25441 aagcaatggc atcacgaatt ttgacccgct tacgcctccc ctttcgtatt ggtttaattg
    25501 aagtttatac cagctgttca gtaggtattt cgctctctcc cgaacatggt tcggacagcg
    25561 cgactattat tcgtcacgcc gacacagcaa tgtacacagc gaaggaaggc ggacgaggac
    25621 aattttgcgt ttttactcca gaaatgaatc agcgggtatt tgaatatctc tggctggata
    25681 ccaacttgcg taaagcactg gaaaacgatc agttggttat cgaaaatcac ctagcgtggc
    25741 gaagtgcgca gtctggaagc actagtacgt tggcagtcac ctgaacgtgg gttgattcca
    25801 ccgttggact tcatttccta cgccgaagag tcagggctaa ttgtgccttt aggccgttgg
    25861 gtgattctcg atgtcgtacg ccaggtggca aagtggcggg ataaaggcat aaacctgcga
    25921 gtggcggtaa atatttctgc acgtcagctc gccgatcaaa ccattttcac cgccctgaaa
    25981 caggttctcc aggaactcaa ttttgaatac tgccctatag atgttgaact gacagagagt
    26041 tgtctgattg agaatgatga actggcactg tctgttattc aacaatttag ccaactaggt
    26101 gcgcaagtgc atctggatga ttttggtacc ggctactctt cactttcgca actggcgcgc
    26161 tttccgatcg atgccatcaa acttgaccag gtttttgttc gagatattca caaacaacct
    26221 gtctcgcagt cactggtccg ggcgatcgtc gctgtggccc aggcattgaa tcttcaggtg
    26281 atcgccgaag gtgtagagag tgcaaaggaa gatgcttttt taaccaagaa cgggatcaat
    26341 gagcggcaag gatttttgtt tgccaaaccg atgcccgccg tcgccttcga acgctggtat
    26401 aaacgctatc tgaagcgcgc ataagtagat gaatatatat acttagcgct atatcccgtc
    26461 attatatatc gtattcctgg atgataatta gccgttgctg ttaaacactt ctcttttagc
    26521 ggcaattttt gcattattca ttttccccct tttgctataa ggatgtcctt gatgtctgaa
    26581 tttgatgccc agcgcgttgc cgaacgtatt gatattgtgt tagatatcct tgtggcaggc
    26641 gattaccact ctgcgatcca taatctcgag atacttaaag cagaactctt gcgccaggtt
    26701 gctgaatcta cgccagacat cccgaagaca ccgtgggaga tttaacaatc gtgccttcag
    26761 gttttcctat ttgaggatgc ccggcgcgtc agtatttttt tatttagtat tataacgtta
    26821 taagaattac agcgatgaat tcccgacaac aaactattct acagatggtc attgaccagg
    26881 gtcaggttag cgtaaccgat ctggcaaaag ccactggagt ttctgaagtt accattcgcc
    26941 aggatctcaa caccctcgaa aaactgagtt acctccgccg tgcacatggc tttgcagttt
    27001 cgcttgatag tgatgacgtc gaaacccgta tgatgagcaa ctatacgctg aagcgtgaac
    27061 tcgccgagtt tgccgcgtca ctggttcaac cgggcgaaac catctttatc gaaaatggca
    27121 gcagcaatgc cctgctcgcc cggactctgg gggagcagaa gaaaaatgtc actatcatca
    27181 cggtcagcag ctacatcgcg catttgctga aagacgcgcc ttgtgaagtc attttgctcg
    27241 gtggcgtgta ccagaaaaaa agcgaaagta tggttggccc tttgacacgc cagtgcatcc
    27301 agcaggtaca tttcagcaaa gcatttattg gtattgatgg ctggcaacct gaaactggat
    27361 tcaccggtcg cgatatgatg cgcaccgatg tggtcaatgc cgtgctggaa aaagagtgcg
    27421 aagcgatagt cctgactgac agctcgaaat ttggtgctgt acattcatac tccatcggtc
    27481 ccgttgagcg attcaatcgc gtgattaccg attcgaaaat acgcgccagc gatctgatgc
    27541 atcttgagca cagtaaactc accgttcacg tcgttgacat ttaaccattt cccttctcta
    27601 cggatgattt gcagtttggc aaatcatccg ctctaagatg attcctggtt gataattaag
    27661 actatttacc tgttattaac actctcaaga tataaaatta ttatcagcga tataacagga
    27721 agtaattatc acgtgcgt
//
LOCUS       DADMGC010000043        27706 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.043, whole genome shotgun sequence.
ACCESSION   DADMGC010000043 DADMGC010000000
VERSION     DADMGC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.043"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..614)
                     /locus_tag="JNZ73_001818"
     CDS             complement(<1..614)
                     /locus_tag="JNZ73_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY8732119.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAG"
     gene            complement(611..1873)
                     /locus_tag="JNZ73_001819"
                     /pseudo
     CDS             complement(611..1873)
                     /locus_tag="JNZ73_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
     gene            complement(1870..2778)
                     /locus_tag="JNZ73_001820"
     CDS             complement(1870..2778)
                     /locus_tag="JNZ73_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY8732120.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2944..3741
                     /locus_tag="JNZ73_001821"
     CDS             2944..3741
                     /locus_tag="JNZ73_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY8732121.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRINVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQP
                     GSCIYLDAGTTTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(3792..4444)
                     /gene="pphB"
                     /locus_tag="JNZ73_001822"
                     /pseudo
     CDS             complement(3792..4444)
                     /gene="pphB"
                     /locus_tag="JNZ73_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(4550..7111)
                     /gene="mutS"
                     /locus_tag="JNZ73_001823"
     CDS             complement(4550..7111)
                     /gene="mutS"
                     /locus_tag="JNZ73_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAY8732122.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLLRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            7187..7366
                     /locus_tag="JNZ73_001824"
     CDS             7187..7366
                     /locus_tag="JNZ73_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732123.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFKIKM"
     gene            7398..7751
                     /locus_tag="JNZ73_001825"
     CDS             7398..7751
                     /locus_tag="JNZ73_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HAY8732124.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(7788..9866)
                     /gene="flhA"
                     /locus_tag="JNZ73_001826"
     CDS             complement(7788..9866)
                     /gene="flhA"
                     /locus_tag="JNZ73_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HAY8732125.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(9940..10950)
                     /gene="hypE"
                     /locus_tag="JNZ73_001827"
     CDS             complement(9940..10950)
                     /gene="hypE"
                     /locus_tag="JNZ73_001827"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HAY8732126.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAAVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(10947..12068)
                     /gene="hypD"
                     /locus_tag="JNZ73_001828"
     CDS             complement(10947..12068)
                     /gene="hypD"
                     /locus_tag="JNZ73_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HAY8732127.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(12068..12340)
                     /gene="hypC"
                     /locus_tag="JNZ73_001829"
     CDS             complement(12068..12340)
                     /gene="hypC"
                     /locus_tag="JNZ73_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HAY8732128.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(12331..13203)
                     /gene="hypB"
                     /locus_tag="JNZ73_001830"
     CDS             complement(12331..13203)
                     /gene="hypB"
                     /locus_tag="JNZ73_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HAY8732129.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(13207..13557)
                     /gene="hypA"
                     /locus_tag="JNZ73_001831"
     CDS             complement(13207..13557)
                     /gene="hypA"
                     /locus_tag="JNZ73_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY8732130.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            13769..14230
                     /gene="hycA"
                     /locus_tag="JNZ73_001832"
     CDS             13769..14230
                     /gene="hycA"
                     /locus_tag="JNZ73_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HAY8732131.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            14355..14966
                     /locus_tag="JNZ73_001833"
     CDS             14355..14966
                     /locus_tag="JNZ73_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY8732132.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPACVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            14963..16815
                     /gene="hycC"
                     /locus_tag="JNZ73_001834"
                     /pseudo
     CDS             14963..16815
                     /gene="hycC"
                     /locus_tag="JNZ73_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
     gene            16818..17714
                     /gene="hycD"
                     /locus_tag="JNZ73_001835"
     CDS             16818..17714
                     /gene="hycD"
                     /locus_tag="JNZ73_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HAY8732133.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIGSPLPQLGDLITLLYLFAIARFFF
                     AISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPL
                     SQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLK
                     QLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDI
                     TPRITWAGFGFAFLAFVSLLAA"
     gene            17732..19417
                     /locus_tag="JNZ73_001836"
     CDS             17732..19417
                     /locus_tag="JNZ73_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HAY8732134.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDDWPDELYPLRKDSMDYRQRPAPTTDAETY
                     EFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAET
                     RMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSH
                     LLNLGLACHFTAFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRHDLLKDDM
                     IQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGLA
                     RDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLM
                     VEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGN
                     TVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            19427..19969
                     /gene="hycF"
                     /locus_tag="JNZ73_001837"
     CDS             19427..19969
                     /gene="hycF"
                     /locus_tag="JNZ73_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HAY8732135.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KENFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            19969..20736
                     /gene="hycG"
                     /locus_tag="JNZ73_001838"
     CDS             19969..20736
                     /gene="hycG"
                     /locus_tag="JNZ73_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HAY8732136.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGILHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            20733..21143
                     /locus_tag="JNZ73_001839"
     CDS             20733..21143
                     /locus_tag="JNZ73_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HycH family protein"
                     /protein_id="HAY8732137.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARHKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            21136..21606
                     /gene="hycI"
                     /locus_tag="JNZ73_001840"
     CDS             21136..21606
                     /gene="hycI"
                     /locus_tag="JNZ73_001840"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HAY8732138.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            21676..22608
                     /locus_tag="JNZ73_001841"
     CDS             21676..22608
                     /locus_tag="JNZ73_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732139.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFDKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEGGTRTDFLQSGYEDFGGNYIAHKCLHIVNL
                     DLMSYSFRTHKDRTRRCGCLLTIRKLKLKNAHHF"
     gene            22592..22903
                     /locus_tag="JNZ73_001842"
     CDS             22592..22903
                     /locus_tag="JNZ73_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HAY8732140.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            22900..23319
                     /locus_tag="JNZ73_001843"
     CDS             22900..23319
                     /locus_tag="JNZ73_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY8732141.1"
                     /translation="MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(23434..24858)
                     /locus_tag="JNZ73_001844"
     CDS             complement(23434..24858)
                     /locus_tag="JNZ73_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY8732142.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQDD
                     EITPNQQGIAFYRPVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAIGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24867..26315)
                     /gene="ascF"
                     /locus_tag="JNZ73_001845"
     CDS             complement(24867..26315)
                     /gene="ascF"
                     /locus_tag="JNZ73_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HAY8732143.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVPWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQPTVAKEVSLN"
     gene            26572..27585
                     /locus_tag="JNZ73_001846"
     CDS             26572..27585
                     /locus_tag="JNZ73_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY8732144.1"
                     /translation="MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEES
                     SYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKH
                     SAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSV
                     WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLI
                     ANGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGF
                     DDIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIA
                     LSR"
BASE COUNT         6350 a         7328 c         7452 g         6576 t
ORIGIN      
        1 cctgcggtca gataacggat cgggcggtca ccgagaataa aaatcagctt tgccgtttcc
       61 tccagctctt ccatattgtt ggcggcttct tgcaggcttt caccgcaaac cactgggcca
      121 tgatttgcca gtaaaaaagc ctgattgtct gctgccagtt ccgccagatc ctgtgcgatg
      181 cgtttatcgc ccggtcggta atagggcacc agcgggacat ttcccatccg catcaccacg
      241 tatggtgtga acggacgaat aacgttgctg ctgtccagcc cttgcaggca ggaaagcgcc
      301 gtcgaccatg tgctgtgcaa atgcaccacc gctttacagc gcggattgtt gcgatacagc
      361 gccagatgaa agagcacctc tttcgagggt ttgtcaccac ttaaccattc gccatccgcg
      421 gcgactttgg aaagccgctg cggatcgaga ttgcccaggc atgaacctgt cggtgtcgcc
      481 agtaaattcc cgtcaggtaa aagcagcgac agattgccag ccgaaccggt tgcatagccg
      541 cgctgaaaga atgaactggc aatccgcgtc atctcctctc gcaaagactg ctctactttt
      601 gcgaaatcgc tcatgataaa aactctcttt gggctcgtga aaaaaaggct tcatcaccga
      661 agttgccaga tttaagggcg agtgagacag gcttatccag tgcgtttacc cacggcacgc
      721 cgggggaaat ggttgggcca atatgaaacc cttttatgcc caggctctgt gtgactacgc
      781 cggaggtctc accacctgcg acaataaagc gtgtcatgcc ttccgctgct aaccgcgccg
      841 ctagttgaga aaacagagtt tctactgcct gactggcttt ttgtgcaccg tattgctgtt
      901 gaattgctgc caatgcgtca gtgctggcgg tggcaaaaac cagtggagca agtacacttt
      961 cctggcccag aacccactct gccagttcgt gagcataagc ggccagagtt tcggttgaga
     1021 ggcagcgtgc cacatcaact tcacgggctg gtgcaatttg acggtaatgt gccacctagc
     1081 ggttggtcat ttgagagcat gaaccggaga gcactacgcc gcgcccagcg agcggacgcc
     1141 ctgcttcgcg agcctggtta ccgttttctt gcgcccactg ccgggccagg ccaatcgcca
     1201 gaccagaacc gcccgttacc agtggggcat cgcgcaaggc ttctccctga atttccagat
     1261 ggtgttcggt cagcgcatca agcaccgcgt agcggtagcc ctcttgctgt aagcgagcca
     1321 gctcttgacg aacggcatcc acaccttgtt cgaaaacatg tgccgaaacg acgccgcagc
     1381 gccctgtgga ttgcgcttca accagacggg gaagatagct gttggtcatg ggatttaccg
     1441 ggtgatggcg catcccggat tcggccagca gttgattcat tacgaacaaa tacccctgat
     1501 aaaccgtacg tccgttgacc ggcagggccg gagagaagac cgtaaacggc gtgtcgagag
     1561 catccattaa tgcatcggta accgggccaa tattaccttt cgccgtactg tcgaaagtag
     1621 agctgtattt gaaatagatc tgtttgcaac cttgctgttg caaccagctc agagccgcca
     1681 gcgattgctg tgtggcttca accaccggac aggagcgcgt tttcaggctg atcaccagtg
     1741 cgtcgattgc ttccggcatt ttacctgttg gaacaccgtt aatttgtacc gttggtagac
     1801 cgttttccac cagaaaactg gcgatatccg tcgcgccggt aaaatcatcg gcgataacgc
     1861 caatcttgat catgatttcg ctcccggtag agtgatgcca gagaaaatct tgataactgc
     1921 gctatcgtct tctttcccgt aacccgcgtt actggcgctg gtgaacatat tcaatgctgt
     1981 tgaggccaat ggcagcggga agtgcagggc tttggctgta tcggcaacca gaccaagatc
     2041 cttaacaaaa atatcgacgg ctgaatgcgg ggtgtaatcg ccatccacca catgacgcat
     2101 ccggttttcg aacatccagg aatttccggc ggcattggtc acgacgtcat acatcacatc
     2161 cagcgggatc cccgcacggg ctgcaagtgc catcgcttcg gctccggcag caatatgtac
     2221 gcccgctaac aactggtgaa taatttttac ggtcgaacct agtcccggtt ctgcacctat
     2281 gcgataaact tttccggcaa cggcttccag cacgggtgcc agtcgttcaa aggcaatatc
     2341 gctaccggag gccatgacag tcatttcacc gttagcggct tttactgcac caccagaaac
     2401 tggcgcatcc agcatttcca gatcgaatcc agccagagcg gtagcaattt cttgcgcatc
     2461 agcactagcg atagtggaag aaaccattac tgccgtaccg ggtttcagat gttgtgcaac
     2521 gcctgtttca ccaaacagca cctgtttaac ctgggccgca ttgaccacca gcaccagcag
     2581 agcgtccagt ttttcggcaa acgtcgcggc gttatcagaa accccgcaag cacctgcctc
     2641 tttcaacgta gcgcaggcat tgctgttcag gtctgcgccc caggtagaaa aacctgcgcg
     2701 gacacatgac agtgctgctc ccattcccat tgaccctaag ccaacgatac cgacatgaaa
     2761 ctcagatccc gttttcatct gctctccttg ttaatttaag tgatattttg tttgacattg
     2821 tgaatataag cgctgggaga taacgatatg gtgagctgat tcacataaat taacattttg
     2881 tgttatttta tgtgaactaa gcgttagttg cgccgcgcac gtttcgcagg caaatagcgt
     2941 agaatgtcag caggacaaag gaaggagcaa aagttgatac ccgtagagcg tcgccaaatc
     3001 atccttgaga tggtagctga aaaaggcatt gtcagtattg ctgaactaac ggacagaatt
     3061 aatgtgtcac atatgaccat tcgtcgggat ttacaaaaac tggagcagca gggagccgtt
     3121 gtgctggtgt ccggaggcgt ccagtctccg ggacgcgtgg cgcatgaacc ttctcatcag
     3181 gtaaaaactg cgctggcaat gacgcaaaaa gcggctattg gcaagctggc ggcaagtctt
     3241 gttcagccgg gaagttgtat ctatctggat gcgggaacga ccacgttagc gatagcacag
     3301 catctgattc acatggagtc actgactgtg gtcacaaacg atttcgttat tgcgaactac
     3361 ttgctcgaca acagtaattg cacaattatt cacactggcg gtgcagtgtg tcgggaaaac
     3421 cgttcctgtg tcggggaagc cgctgcgacc atgctgcgca gcctgatgat tgatcaggct
     3481 tttatttctg catcgtcatg gagtgtgcgg gggatttcta cgccagcgga agataaagtc
     3541 acggtgaaac gggcgattgc cagtgctagc cgccagcgag ttttggtctg tgatgcgacg
     3601 aaatatggtc aggtggcgac atggctggcg ttaccattaa gcgagtttga tcagattatt
     3661 acagacgacg gtttgccgga gagtgccagt cgcgcgctgg cgaagcagga tctctctttg
     3721 ctggtagcga aaaatgaata atggcctgca ataacatctg gttactcatg cttcacagaa
     3781 gaagcatgag actaccttat tttataaaat gacagccgcc cacttttcgg cgatccggta
     3841 tcaatataaa tctggttagc gaacgtctga atgttatcaa acatcatatg tccaaatata
     3901 aaataatcag cgccgtttat ttgttgtaac tcgccattaa gcgagttctg cacacgataa
     3961 acaggccaga gtaattcgct ctccgctatt tctttgtcaa agagatattc atcacccgga
     4021 taatctgcat gtgcgatgac atattttatt atgtcgttag tgatttcaat aatatgtgga
     4081 aggtgatgga atttcagcaa cagatctatt gcctcttgtt gcaatcattt aaatcgaaaa
     4141 accagtcacc accgctggca agccacatat tgccatcgcc ggtcgcgaac gcatccagcg
     4201 ccattgcttc gtggttgcct ttaacggaga taaaccaggg ctggtttagc aggcgcagga
     4261 cgtcaagact ctccggccca cgatcaatat tatcgccgac agaaataagt aagtcggttt
     4321 cggggaaaaa agagagttga tgtaagcggg attgtaataa ctgatattca ccatgaatat
     4381 caccaacgac ccatatatgg cgatagtgat gggcattgat tttttgatag cgtgtagatg
     4441 gcatggtttt accctgtaaa ataagctttc ctattataca gggtattttt atttgattcg
     4501 tcagttgtcg ttaatattcc cgatagcaaa agactatcgg gaattgttat tacaccagac
     4561 tcttcaagcg ataaatccac tccagcgcct gacgcggagt cagtgaatcc gggtcgagat
     4621 tttccagtgc ttcgaccgca ggcgaagttt cttctggcac tgacagcaaa gacatctgcg
     4681 taccatccac ttgcgtcgcg gcggcgttcg gcgaaatgct ttccagctca cgcagttttt
     4741 gccgtgcgcg cttaataacc tctttcggta cgcccgccag agcagcaacc gccagaccgt
     4801 agcttttgct cgccgcgccg tcctgcacgc tgtgcataaa ggcgatagtg tcgccgtgct
     4861 ccagcgcatc gagatgtacg ttggcgacgc cttccatttt ctccggtagc tgggtcagct
     4921 cgaaatagtg ggtggcaaac agcgtcaatg ccttaatctt attcgccaga ttttctgcgc
     4981 acgcccacgc cagcgacaga ccatcgtagg tggacgttcc acgcccgatc tcatccatta
     5041 acaccagact gtattcggtg gcgttatgta aaatattggc ggtttcagtc atctccacca
     5101 taaaggttga gcgcccggaa gccaggtcat ctgccgcgcc tacgcgggta aagatgcgat
     5161 cgataggtcc aatctcgact ttttgtgccg gtacatagct gccgatgtag gccatcagcg
     5221 caatcagtgc ggtctggcgc atataggtac ttttaccgcc catgttcggg ccggtaataa
     5281 tcaacatacg gcgctgcggc gacagattca gcgggttagc gataaatggc tcattcagta
     5341 cttgttcaac taccggatgg cgaccttcgg taatgcgaat gcccggttta tcaatgaagg
     5401 tcgggcaggt gtagttcagg gtataggccc gttccgccag gttaaccagc acgtcgagtt
     5461 ccgccagcgc gctcgcgctc tgttgcaacg cttccagatg cggcaacagc aggtcgaaca
     5521 gctcttcata aagctgtttt tccagtgcca gtgctttgcc ttttgaggtg agaactttat
     5581 cttcgtactc ttttagctct ggaatgatgt agcgctcggc gtttttcagc gtctggcgac
     5641 gcatgtagtt gatgggtgcc agatggcttt gcccacggct gatttgaatg tagtagccgt
     5701 gcaccgcatt aaagccaact tttagcgtgt ccaggccggt acgttcacgc tcgcggactt
     5761 ccagacgctc cagataatcg gtcgcgccgt cagccagcgc gcgccactca tccagctctt
     5821 cgttatagcc cgatgcgata acaccaccgt cgcgtaacag caccggcggt gtgtcgatga
     5881 ttgctcgctc cagcagatcg cgcagctcgg caaactctcc catcttctca cgtagcgcct
     5941 gtaccggtgc actatcgaca gtttctaact gcgcacgcag ctccggcagt tgctggaaag
     6001 cgtggcgcat acgggccaga tcgcgtgggc gagcagttcg taaagccaga cgtgccagaa
     6061 tacgttccag gtcgccgacc tgacgcagta ccggctgcaa ctcggcggtg aaatcctgca
     6121 atgcgccaat agtttgctgg cgctcaagca acacgcgagt atcgcgcact ggcatatgca
     6181 gccagcgttt cagcatacga ctacccatcg gcgtgacggt gcagtcgagc acagaagcca
     6241 gcgtattttc cgcaccgccc gccaggttct gagtaatttc caggttacga cgtgtcgcgg
     6301 catccataat gatgctgtcc tgctcacgtt ccatggtgat agaacgaata tgcggcaggg
     6361 tcgtgcgttg ggtatctttc gcatactgca acagacaacc ggcagcacaa agtccgcgcg
     6421 gcgcgttctc gacgccaaaa ccgaccagat cgcgggtgcc aaattgcaga ttcaactgct
     6481 ggcgcgcggt gtcgatttca aactcccaca gcgggcgacg gcgcaggccg cgacggcctt
     6541 caatcagcga catctcggcg aaatcttctg catacagcag ttccgccgga ttagtgcgtt
     6601 gcagctctgc cgccatcgtt tcgcggtcgg ccggttcgct caggcgaaaa cgcccggagc
     6661 tgatatccag cgtcgcgtag ccgaaacctt tgctgtcctg ccagatagcc gccagcaggt
     6721 tgtcctgacg ctcctgtaac agggcttcat cgctgatggt gcctggcgta acgatacgca
     6781 caactttgcg ctcaaccgga cctttgctgg tcgccggatc gccaatttgt tcgcagatgg
     6841 caacggactc tccctgattc accagtttgg cgagatagtt ttccaccgca tggtagggaa
     6901 tccccgccat cgggatcggc tctcccgccg aagcaccgcg tttggtcagt gaaatatcca
     6961 gcagttgcga cgcgcgtttt gcgtcgtcat aaaacagttc ataaaaatca cccatccggt
     7021 aaaacagcag gatctcggga tgctgggctt tcagcttgag atactgctgc atcatgggcg
     7081 tatgggcgtc gaaattttct attgcactca tggggttatg tccggttccc tgatattaaa
     7141 tggggtgtga tggtttttat tttcgttgta atcacataag gcgcacatga taccggagtt
     7201 aatcatttca ggggaagaag cgaacggcga gttcttgatg ctggttccgg gaaagatgct
     7261 gttttgcagc ttttgcgtga tgcttatgaa gcaagtacgc aaaattgtat cgattggcga
     7321 acccttcttc tttagagtca tagtgccctt taagattaag atgtaatata aatgcatctt
     7381 aatgaggtgg aggcagcatg tccggcaagc gtatctctcg tgaaaaactg acgattaaaa
     7441 aaatgatcga tctttatcaa gcgaaatgcc cgcaggcgtc agcggagccg gagcattacg
     7501 aggcgttgtt tgtttacgcg caaaagcggc tggataaatg tgtgttcggc gaagagaaac
     7561 cagcctgtaa gcagtgtccg gtgcactgtt accagcctgc aaagcgtgaa gagatgaaac
     7621 agattatgcg ttgggccggg ccgagaatgc tgtggcgtca tccgatctta accgtgcgtc
     7681 atctcatcga tgacaagcgt ccggtgccag aactgccgga aaaatatcgt ccgaaaaaac
     7741 ctcgtgaata gtatgccgga caggcagatc tgtccggcaa tttgcagtta aatcaatgcc
     7801 gatttatcaa ttcccagccg cttcatccgt gacagcaggg tcgtgcgttt cagccccaga
     7861 cgttgcgcag cgcctttagg cccggcaacc acgctgttgg tttctttcag cacgcgcaca
     7921 atcaactgat attcatcttc gccctcctgg gcgacaaccg ttgcggcagg cggcgtttca
     7981 ggctccggta aagcaatatc tggcaatgac agctgcagca cgttgccgcg tgttagcaat
     8041 actgcgcgct caatgacgtt ttccagttcg cgtacgttac ccggccactc catgttgctc
     8101 aaggtgcgca gcgtctcggc aggaatgctg tcgatattgc gccccagacg acgggcaatt
     8161 ttgaaggtaa aggctttcgc cagcagcgga atatcttccg gacgctcgcg tagtggcggc
     8221 aggtgaatcg ggaatacgtt caggcggtaa tagagatcgc tacggaactc tcggtcggcg
     8281 accatttttt tcagatcgcg gttagtcgcg gcaattaaac gtacgtccgt ctgaatgatt
     8341 ttgttgctgc cgagacgttc aaactcctgt tcctgcaata cacgcagcaa cttcggctgt
     8401 aactccagtg gcatatcgcc cacttcgtcg aggaacagag agcttttatc cgccagttca
     8461 aaacgaccaa tgcgctgggc gctggcaccg gtaaaagccc cacgctcatg accaaacaga
     8521 tcgctttcca gcaatccggc aggcatcgcc gcgcagttca ttttgaccat gcggcgatta
     8581 ttacgcccac tgagattatg gatcgcacgg gcaatcagct ctttacccgt gccagtttca
     8641 ccgaggatca gcacggtact gtcactttgc gccaccattt caacttgttt aagcacgctg
     8701 tacatggctt cgctgcggcc aataatctcg ccaaattcac tatcaacatt gttgagttgc
     8761 tcggtcaggg cgaggttttc atcaaccagc cgttctttca gacgatggat ttcctgatag
     8821 gcgagggcgt tatcgacagc gattgccaca cgttcggcaa tctggcgcag caaattcaga
     8881 ttggtagtgg taaacacttt ctcttcgcat tgcgccagtt tcagcacgcc cagcatggtg
     8941 tcgccagaca tcagcggtaa caggcacaag gtttgaatct ggttgcccca ggtgtcgaac
     9001 aacatgcgtt catagggggc taaatcgtcc cgctcgtaga gattgatcag cagcatctct
     9061 ttacttttga acacgcgttc ggtgagggtt ccggcttcat cgacttcgct ctgttcgtgg
     9121 gcgggatgtt gtttatcaag atagtgagtg gagtagatgt tgagtttgtt tttacggtgg
     9181 ctgcgtaaga caatgctgat atcgtcaatg tcgaaatagt aatggatttc tttggcgact
     9241 tcgctgacca gttcgtccat atccaggcgg gaaagcaccg cgttggtaat ggcaaccagg
     9301 atgcggaagt tatcgcgctc ccggcataac aactcatagt cgacattgtt gacgacgcgg
     9361 ctctggattt gttcggtgac gacagaaacg atctgcgtaa atgtttgcag acgattgaac
     9421 tctttttcgc tccagggacg atcgtcataa cgaataaatt cacagccacc aaaaatatgc
     9481 cctcccgccg ccagtggcat caggcaatag tgaccaaatt tggggtatag cccacctgcg
     9541 gccagctgcg gccaggtttc acaaaattct tcgtaactgc aatgcagcgt atcagggcgc
     9601 gacaaaatgc tgcgtaccgg accgtgtgcc agaacagttt cgtcttcata tttaatgggg
     9661 gtgtcttttt cacgcgacgc gtaataagac gcacgttgag tctgcgcttg ccacaacaca
     9721 atagccgcgt tgtcggcgag cgcagaacgc tttaccagtt gcgaaagagc ctcacacagc
     9781 gaggccagat cgggctgctg caatagtgtc cgagtgatgt cgaacaaccc ttgctgtccg
     9841 agatcactca tcggtgtata tgacattttg ttcttccagg aaagcaccgc aaagcggtgc
     9901 aaaaggtcat tgactaacta taggagattt agaattttat tagcatatac gcggaagcgg
     9961 ttcggcgtgt ggtaaatcga gggttcgttt cacgccatac agaccggcaa gacgaacacc
    10021 tttacgttcc accacttcac caatcagcgc cgcgtctttc cccagtggat gggaatgtaa
    10081 cgctgccagc acttgctctg ccgcgttgcg ttcaacggcg atcaccagtt taccttcgtt
    10141 ggcaaagttc agggcgtcca gtcccagcaa ttcgcaaacg ccgcgcacgg caggtttaac
    10201 cggcagcgct gattcagaaa tttcaatacc acaaccgcag gctgccgcga actcatgaac
    10261 caccgcgttt acaccaccac gggtggcatc acgcagcgct ttcacgccgg gaatgtcacg
    10321 cagcgtctga ataagcggcg tcagcaccgc gcagtcgctg accagttcgc catccagccc
    10381 cagctgctca cgcaggttaa ggatagtcgc cccgtggtcg ccgagtgtac cgctaaccag
    10441 caatacatcg cctgcggtta gcgtctgtgc gccccagtga atattcgccg gaattgcgcc
    10501 catgccagcg gtgttgataa acagtttatc tgccgcgccg cgctgcacca ctttagtatc
    10561 gccagtaacg atggcaatgc ctgccgtgcg ggcggtttct gccatgctgg tcactgcggc
    10621 tttcagtgtc tccatcggca atccttcttc gaggataaag ccacaggaga gatagcgcgg
    10681 aatagcgcca ctgaccgcaa cgtcattcgc ggtgccgcaa atcgccagct tgccgatatt
    10741 accgccagga aagaacagcg ggtcaatgac gtaactgtcg gtggagaacg ccagacggtc
    10801 gccttccgct accagctgcg ccagatcaag acgtgcctga tcttcctgct ctgccagcca
    10861 cgggttggca aaggcttcca taaacaggct gttgattaat tgctgcatcg cctggccgcc
    10921 gctgccgtgg gcgagttgga tattattcac gcttcactct cctgctgacg atactgatac
    10981 cacgcggcgc acgctccttc ggaggagacc atcagtgcac caaatgcggt ttgaggatta
    11041 caggtgttac caaacagcgg gcattgatgc ggcttacatt tgcccgttaa tacctcacca
    11101 caacgcgcgc gcgggtcatc gcagacctgc tgcggtgccg ggcggaaatg tgcttcggca
    11161 tcgaatcgtt gataatccgg cgtcaggtgc acgccagaag attcaatcac gcctaagccg
    11221 cgccattcgc tgtcgccgtt gacacagaac acatcggcaa tcgcctgttg cgccagcagg
    11281 ttaccggcat ccggtaccac ccgacgatac tgattctcta ccttgctgtg ggccgctatt
    11341 ttctgctcca ccagcatgac cacgccttgc agtagatcaa ggggttcgaa accagcgacc
    11401 accagcggac gatgaaaatc gctggcgata aaattatagg catcagtgcc gataaccata
    11461 ctaacgtggc ctggcgcgag gaacgcgtcg ataccgttat ccggctgttc cagcaaactg
    11521 cgcagcgtcg ggataagcgt aatgtgctgg cagaagaagt aaaaattctg cacatcacgc
    11581 gctttcgcct gttgcagagt gatggcggtg gtcggcatgg tggtttcaaa acctaagccg
    11641 aagaacacca ctttgcgggt tggattctcc tgcgccagtt tcaacgcatc catcggcgag
    11701 taaacgatgc gcacatcggc accgcgtgct tttgcctgca acagcgatcc ctgtttccct
    11761 ggtacgcgca tggcgtcgcc aaaggtacag aagatgactt ccggatggct ggcaatctcc
    11821 acgcaggtgt cgattctacc catcggcagt acgcacaccg ggcaacccgg accgtggata
    11881 aactcaacgt tttcaggcag taactggtcg aggccgaatt taaaaatggc gtgggtatga
    11941 ccgccacaca cttccataat ccgcagaggg cgttcggcgg tgtaagagag atgtgaagca
    12001 cgttcgcgca gatgctcaat taactgcatc acctgttccg gcgcgcgata ttcatcaaca
    12061 aaacgcatta tttttcctcg ccatacaaca gcgcaccgac atccggctca acgtcaaaca
    12121 tgttttgtaa ggcgtcgaga gtgtcgcgtg cttcggcttc attaattacg ctcatggcaa
    12181 agccaacgtg aaccagtacc cactggccca cgcgcggctg accgttttca tcgcagctgc
    12241 cgactaacgt taaatcgaca tcgcgctgaa tgccgcagac gtcgactttc gcctggttgc
    12301 cgtcaatggt gcggatctgg ccgggaacgc ctatgcacat cgctgtgtct ccagccagtt
    12361 cagccactgg tccatccctt cgccgctggt ggcggaaata aggatgattt caatttctgg
    12421 attgacttcg cgggcgcagg cgatgcactt ctcaacgtca aagttgagat acggcaacag
    12481 gtcaactttg ttgagcagca tcagcgaggc ggcggcaaac atatgcggat atttcagcgg
    12541 tttgtcttca ccttcggtaa cggaaagcac cgctactttg tgtttttcac cgagatcgaa
    12601 gctggccggg catacgaggt tgccaacgtt ttcgataaac agaataccgt tatcgtccag
    12661 tggcagacgc ggtgcggcgt cggcaatcat ctgtgcgtca agatggcagc ctttaccggt
    12721 gttcacctga atcgctggtg taccggtagc gcgaatgcgt gcggcatcgt tcacggtttg
    12781 ctggtcgcct tcaataaccg cgcacggaac gctgtctttc aggcgcatta aggtttccgt
    12841 cagcagggtg gttttaccgg aaccagggct ggaaaccagg ttgagcacca gttgcttgcg
    12901 ggcagcaaag cgcgcgcggt tgcgttcagc cagacggttg tttttgtcca gcacgtcaat
    12961 ttcgacttcc agcatccgac gctggctcat gcccggtgtg tgagtgcccg cttcgccatg
    13021 accgtaatgc aggtcgcctt cttcagtctg gctgggggta aattcaggcg ctttaatgcc
    13081 ggtgattttc atctgcgggc gtgccgccgg ggcaaatggc gcgctacgaa acgcggaatg
    13141 agggttatgt tcatcaccct cgatatacag gttgccttca ccgcaaccgc atgttgtaca
    13201 catcgctcac tcctggtcta tttctatccg ccgaatctgt aaaccgtcgt ctgccacaat
    13261 ctgtagcatg tcaccatgac actgtggaca gcggcggacg cgctgggtca gtagcgtcac
    13321 atactgttgg catgtttcac accagcattc ggcctcttgt tcttcgaggt gcagtttaca
    13381 accttccgcc acgctgccgc ggcaaaccag atcaaaacaa aaggcaagag agctggtttc
    13441 gacacaagaa aatgcgccaa ttttgagcca gaccccagtt acgcgttttg cgccgtgttt
    13501 tgcggcctgc tgttcgatca attccagtgc ccgttggcag agggttattt cgtgcatatc
    13561 gcctcccatt aactattgcc agctacaagc aataattgcg ccagtgttga ttatccctgc
    13621 ggtgaataat gtcgatgatg tcgaaatgac acgtcgacac ggcgacgaaa ttcatcttta
    13681 gcttaaaaat ctctttaata acaataaatt aaaagttggt acaaaaaatg cttaaagctg
    13741 gcatctctgt taaacgagta acctgacaat gactatttgg gaaataagcg agaaagccga
    13801 ttacatcgca cagcggcatc gtcgcctaca ggaccagtgg cacatctact gcaattcgct
    13861 ggttcagggg atcacgttat cgaaagcgcg cctgcatcac gccatgagct gcgcgccgga
    13921 caaagaactc tgtttcgtcc tttttgaaca ttttcgtatt tacgtcaccc tggcggatgg
    13981 ctttaacagc cacaccatcg agtattacgt cgaaacaaaa gatggcgaag acaaacagcg
    14041 gattgcgcag gcgcaactga gcattgacgg catgattgat ggcaaggtca acatccgcga
    14101 tcgcgaacag gttctggaac actatctcga aaaaatcgct ggcgtttacg acagcttata
    14161 caccgccatt gaaaacaatg tgccggtgaa tttaagccaa ctggtaaagg gacaaagccc
    14221 ggcagcatga gctgaggctt tgcccgtttt gcaggcgtta cgcctgtttg gggatgggcg
    14281 tgtcgatgag tgtcgaaaat gacatttcat cggcatgttt tcgtcaaaaa tgacaatcac
    14341 ctgaggaatg cctggtgaat cgttttgtaa ttgccgactc cacgctctgt atcggctgcc
    14401 acacctgtga ggccgcctgt tcagagacgc atcgccagca cggcctgcaa tcaatgccgc
    14461 gcctgagagt gatgctcaat gaaaaagaat ctgcgccgca gctctgtcac cactgtgaag
    14521 atgcaccctg cgcggtggtc tgcccggtta acgccatcac ccgcgttgat ggggccgtgc
    14581 agttgaatga aagtctgtgc gtaagctgca aactgtgcgg catcgcctgc ccgtttggcg
    14641 caattgaatt ttccggcagc cgtccgctgg atattccggc aaacgccaat accccgaaag
    14701 cgccaccggc accgcctgct ccggcgtgtg tcagcacatt gcttgactgg gtgccaggta
    14761 ttcgcgcgat cgccgtcaaa tgtgaccttt gtagctttga tgaacaaggt ccggcctgtg
    14821 tgcggatgtg cccgactaaa gccctgcatc tggtggataa caccgatatc gcccgcgtca
    14881 gcaaacgtaa gcgtgagctg acttttaaca cggactttgg cgatctcacc ttgtttcagc
    14941 aggctcaaag tggagaggct aaatgagcgc aatttccctg atcaatagcg gcgtggcatg
    15001 gtttgtcgcc gccgctgttc tggcatttct cttttctttt caaaaagcgc taagtggctg
    15061 gatagccgga attggcggcg cggttggtag tctgtatacg gcagccgcgg gcttcactgt
    15121 actgactggc tcggttggcg tgagcggtgc gctgtcgctg gtaagctacg atgtgcaaat
    15181 ctccccgctt aacgcgattt ggctgattac cctcggtctt tgcggtctgt ttgtcagcct
    15241 ctataacatt gactggcatc gccacgcgca ggtgaagtgc aacggcttgc agatcaatat
    15301 gttgatggct gccgccgtct gcgccgtcat cgccagcaac ctcggcatgt tcgtggtaat
    15361 ggccgaagtc atggccttgt gtgcggtgtt cctcaccagc aacagcaaag agggcaaact
    15421 gtggtttgcg ctggggcgtc ttggcactct gctgctggtg attgcttgct ggctgctgtg
    15481 gcagcgttat ggcacgctgg atctgcgcct gctggatatg cgtatgcaac agctgccgct
    15541 cggttccgat atctggctgc tcggttccga tatctggctg ctcggtgtga ttggctttgg
    15601 cctgctggct gggattattc cgctgcacgg ctgggtgccg caggcacatg ctaacgcctc
    15661 tgcgccagct gccgcgttgt tttctacggt agtcatgaaa attggcctgc tgggcatgtt
    15721 aaccctgtca ctgctgggcg gtaatgcacc gctgtggtgg gggatcgcgc tgctggtgct
    15781 cggcatgatc accgcgttcg tcggtggtct gtatgcgttg atggaacaca acatccagcg
    15841 cctgctggct taccacaccc tggaaaatat cggcattatc ctgctggggc tggggctggg
    15901 gctggcgtaa cgggtatcgc gctcgaacaa ccggcgctga ttgctcttgg cctggtcggt
    15961 ggtctgtacc atctgcttaa ccatagcctg ttcaaaagcg tactgttcct cggggcgggg
    16021 agcgtctggt tccgtaccgg tcatcgcgat atcgaaaaac tcggtggtat tggcaagaaa
    16081 atgccggtta tctccatcgc catgttagtc gggttgatgg caatggctgc gctgccgcca
    16141 ctgaacgggt ttgccgggga atgggttatc tatcaatcct tcttcaaact gagcaatagc
    16201 ggcgcgtttg ttgcccgtct gctggggccg ctgctcgctg tgggtctggc aattaccggt
    16261 gcgctggcgg tgatgtgtat ggcgaaagtc tatggcgtca cgttcctcgg cgcgccgcgt
    16321 accaaagaag ccgaaaacgc cacctgtgcg ccgctcctga tgagcgtaag cgtagtggca
    16381 ctggcgattt gctgcgtaat tggcggtgtt gctgcgccgt ggctgctgcc gatgctctct
    16441 gctgctgtac ctctgcctct ggagcctgct aacaccaccg tttctcaacc aatgatcaca
    16501 ttgctgctga ttgcctgccc gctgctgcca ttcatcatta tggcgatttg caaaggcgac
    16561 cgcctgccgt cacgttcccg cggtgcggcc tgggtgtgcg gctacgacca tgaaaaatca
    16621 atggtgatta ccgctcacgg ttttgccatg ccggttaaac aggcgtttgc gccagtgctg
    16681 aaactgcgca aatggctgaa tccggtgtcg ctggtgccgg gctggcagtg cgaaggtagt
    16741 gcgttgctgt tccgccggat ggcgctggtt gaactggcgg tactggtggt gattattgtt
    16801 tcacgaggag cctgagaatg agtgttttat atccgttaat tcaggcgctg gtgttatttg
    16861 ccgttgcgcc gctgctctcc ggtattaccc gcgtggcgcg cgcccgtctg cataaccgtc
    16921 gcgggccggg cgtgttgcag gagtatcgcg acattatcaa actgctgggc cgccagagcg
    16981 ttggcccgga tgcctctggc tggatgttcc gcctgacgcc gtatgtgatg gtgggcgtca
    17041 tgctgactat cggttctccg ctgccgcaac tgggcgattt gatcacctta ctgtatctct
    17101 tcgccatcgc gcgtttcttc tttgccattt ccggtctgga taccggtagc ccgtttaccg
    17161 ctatcggtgc gagccgtgaa gcgatgcttg gcgtgctggt cgaaccgatg ttgctgcttg
    17221 gtctgtgggt tgccgcacag gttgccggtt ccaccaacat cagcaacatt accgacaccg
    17281 tttatcactg gccgctgagc caaagcatcc cgctggtgct ggcgctctgc gcctgtgcgt
    17341 tcgccacttt tatcgaaatg ggcaaactgc cgttcgacct ggcggaagcc gagcaggagt
    17401 tgcaggaagg tccgctctcc gaatacagcg gtagcggctt tggcgtcatg aaatggggta
    17461 tcagcctgaa acagctggtg gtgttgcaga tgttcgtcgg ggtgtttatt ccgtggggac
    17521 aaatggaaac cttcaccgtc ggtggactgc tgctggcgct ggtgattgcc atcgtcaaac
    17581 tggtggtcgg cgtcctggtt atcgcgctgt tcgaaaacag tatggcccgt ctgcgtcttg
    17641 atattactcc gcgcattacc tgggctgggt ttggctttgc atttttagcg ttcgtctcct
    17701 tgctggcggc gtgattaaag agagtttgag catgtctgaa gaaaaattag gtcaacatta
    17761 tctcgccgcg ctgaatgagg catttccagg cgtcgtgctg gaccacgcct ggcagaccaa
    17821 agatcagctg actgtcaccg taaaggtgaa ctatctgccg gaagtggtgg agtttctcta
    17881 ctacaagcag ggtggctggc tgtcggtgct gtttggtaac gacgaacgca aactgaatgg
    17941 tcattacgcc gtttattacg tgctgtcgat ggagaagggc accaagtgtt gggtaacggt
    18001 tcgcgtcgaa gttgacgcca acaaaccgga gtatccgtcc gtgacgccgc gcgttccggc
    18061 ggcggtgtgg ggcgagcgcg aagtgcgcga tatgtacggt ttgattccgg ttggtctgcc
    18121 ggatgactgg ccggatgaac tttatccgct gcgtaaagac agcatggatt atcgtcagcg
    18181 tccggcaccg actaccgatg ctgaaaccta cgagttcatc aacgaactgg gcgacaagaa
    18241 aaacaacgtc gtgccgattg gtccgctgca cgtcacttct gacgaaccgg gtcacttccg
    18301 tctgttcgtc gatggcgaaa acattatcga cgccgactac cgtctgttct acgtccatcg
    18361 cggtatggaa aaactggcgg aaacccgcat gggttataac gaagtgacct tcctctctga
    18421 ccgtgtgtgc gggatctgcg gctttgccca cagcaccgcc tacaccacgt cggtggaaaa
    18481 cgcgatgggt attcaggtgc cagaacgcgc gcagatgatc cgcgccattc tgctggaggt
    18541 ggaacgcctg cactcgcatc tgctcaacct cggcctcgcc tgtcacttta ccgccttcga
    18601 ctccggcttt atgcagttct tccgcgtgcg tgaaacctcc atgaaaatgg cagagatcct
    18661 taccggtgcg cgtaaaacct acggcctgaa cctgatcggc gggattcgtc acgatctgct
    18721 gaaagatgac atgatccaga cccgccagct ggcgcaacaa atgcgtcgtg aagtgcagga
    18781 gctggtggat gtgctgctga gtacgccgaa catggaacaa cgcaccgtcg gcattggtcg
    18841 tctggacccg gaaatcgccc gcgatttcag taacgttggc ccgatggttc gcgccagcgg
    18901 tctcgcccgc gatactcgcg ccgatcaccc gtttgtcggt tatggcctgc tgccaatgga
    18961 agtccacagc gagcagggct gcgacgttat ttcccgtctg aaagtgcgaa tcaacgaggt
    19021 ctataccgcg ctgaatatga tcgactacgg tctggataac ctgccgggcg gtccgctgat
    19081 ggtggaaggc tttacctaca ttccgcaccg tttcgcgctg ggctttgccg aagcgccgcg
    19141 cggcgacgat atccactgga gcatgaccgg cgacaaccag aagctgtacc gctggcgctg
    19201 ccgtgccgcg acctacgcga actggccgac cctgcgctac atgctgcgtg gcaataccgt
    19261 ttccgatgcg ccgctgatta tcggtagcct ggacccttgc tactcctgta ccgaccgcat
    19321 gaccgtagtc gatgtgcgta agaagaagag caaagtggtg ccgtacaaag aactcgagcg
    19381 ttacagcatt gagcgtaaaa actcgccgct gaaataagga atcgccatgt ttacctttat
    19441 caaaaaagtc atcaaaaccg gcacggcgac ctcgtcttat ccgctggagc cgattgcggt
    19501 tgataaaaac ttccgtggta agccagagca gaacccgcag cagtgcatcg gctgcgcggc
    19561 ttgcgtcaat gcctgcccgt caaacgcctt aacggttgaa actgacctcg ccacaggaga
    19621 gctggcgtgg gagtttaatc tcgggcgctg catcttctgt ggacgctgcg aagaagtttg
    19681 cccgacggcg gcgatcaaac tgtcgcaaga gtacgaactg gcggtgtgga agaaagaaaa
    19741 cttcctgcaa cagtcccgct tcgcgctgtg caactgccgc gtctgcaatc gtcctttcgc
    19801 cgtacagaaa gagatcgact acgccattgc gctgcttaag cacaatggtg acagccgcgc
    19861 ggaaaaccac cgcgaaagct ttgagacttg cccggaatgt aagcgccaga aatgcctggt
    19921 gccgtccgac cgtattgaac tgactcgcca tatgaaagag gccatctgat gagcaattta
    19981 ttaggccccc gtgacgccaa cggcattccg gtcccgatga cggtggatga atccatcgcc
    20041 agcatgaagg cgtcgttact gaaaaaaatc aaacgttctg cctatgttta ccgcgtggac
    20101 tgcggcggct gcaacggctg cgaaatcgaa attttcgcca cgctttctcc gctgttcgat
    20161 gcagaacgct tcggcattaa agtcgttcct tcaccgcgtc atgcggatat tttactgttt
    20221 accggcgcgg tcacccgtgc aatgcgatcc cctgcgctgc gtgcgtggca gtccgcgccg
    20281 gacccgaaaa tctgtatctc atacggtgcc tgcggtaaca gcggcgggat cttacacgat
    20341 ctctactgcg tgtggggcgg tacggataaa attgtccctg tggatgttta tatccctggc
    20401 tgcccgccaa cgcctgccgc cacgctgtac ggctttgcaa tggcgctcgg cctgctggag
    20461 cagaaaattc acgcccgtgg gccgggtgaa ctggatgaac aaccggcgga gatcctgcat
    20521 ggcgatatgg tgcagccgct gcgcgtgaaa gtggatcgcg aagcacgtcg cctggcgggt
    20581 tatcgttacg gtcgtcagat tgccgatgat taccttacac agttagggca gggcgaagaa
    20641 caggttgcac gctggctgga agcggaaaac gatccgcgtc tgaacgagat tgtcagccat
    20701 ctgaatcatg ttgttgaaga ggcgcgtatc cgatgagtga aaaggtggtg ttcagtcaac
    20761 tgagccgtaa atttattgat gagaacgatg ccacgcccgc cgaggcgcag caggtggtct
    20821 attacagcct ggcgattggt caccaccttg gggttatcga ttgcctggaa gcggcgctca
    20881 cctgcccatg ggatgaatat ctggcatgga ttgccactct ggaggcgggc agcgaagccc
    20941 gccacaaaat ggaaggcgta ccgaaatatg gcgagatcgt catcgacatt aaccatgtgc
    21001 cgatgctggc caacgcattc gataaagccc gggcagcgca aacttcgcag cagcaggaat
    21061 ggagtacaat gctgttaagt atgctgcatg atattcatca ggaaaacgcc atctatttga
    21121 tggtgaggag actgcgtgac tgacgtttta ctctgtgttg gcaatagcat gatgggcgat
    21181 gatggcgctg gcccgctgct ggcggaaaag tgcgccgccg cgccgaaagg taactgggtg
    21241 gtgattgacg gcggtagtgc accggaaaac gacatcgtcg ccatccgtga actgcgcccg
    21301 acacgactgc tgattgtcga cgccacggat atggggctaa accccggcga aatccgcatc
    21361 atcgacccgg atgatatcgc cgagatgttt atgatgacta cccataacat gccgttgaat
    21421 taccttatcg accagttaaa agaagacatt ggtgaagtga ttttcctcgg cattcagccg
    21481 gatatcgtcg gcttttacta cccgatgacc cagccgatta aagatgcggt agaaaccgtt
    21541 tatcaacgac tggaaggctg ggaaggaaat ggtggatttg cgcagttagc ggtggaagaa
    21601 gaataattct ccatcaggga attcagggaa ttcagggaat tcagggaatt cagggaattg
    21661 aacatcagaa cagggatgtg cgctttttta ttgagcttag tgctgcctgc acaggccacc
    21721 agctttaccg aatatctgcc aatgagtgac agcgaatacg cgcaaaagcg ggcgctgaaa
    21781 cctctcctga ccatgcctta tgatgccgag caaacctggc attttaggaa agtgggcgtt
    21841 gctggcgtga cgcttgagaa gatgccaaat gaagaagatt actggcaatt aaccgctaaa
    21901 gaccgcgccg gtaaatcctg gactgtgcct gtgggtatgc tggcaaacat agcgggcaaa
    21961 gggcaactct atcgcgccga tcttgatcgt aacggcattc aggatctggt tatctggtta
    22021 ccaagtaccg gtaatggtct ggctccttat gcacatttaa ttcttatgac gttcacgcgc
    22081 gaaggccgtc cctgtgtctt tgaacccagg ggattctaca ccgcaagcaa aactggcgta
    22141 gatgatttgc tggatttgca aggcaatggt cacacgcaat tgctggatat gcagtttaat
    22201 tctggttact ggataaccag tctgtatcag gtaaaagacg ccaaatggca gcgtgttcac
    22261 ggctggtttg acaagttaag ctatcccgcg ctcacgcgtt ttacttatac ccccaaccgt
    22321 aaactcgtcc tcaaacccat cgccgggcgc gacccgcaaa cggaagatct ggcgcagacg
    22381 cagcgttgct tgataaaggg ggatgtgctt gaggggggaa ccagaactga ctttttgcag
    22441 agtggctatg aggattttgg gggtaattat atcgcgcaca agtgtttaca tattgtgaac
    22501 ttggatttga tgagctatag tttccgaaca cataaggata gaactcgcag atgtggctgt
    22561 ttacttacta taaggaagtt aaaacttaaa aatgcacatc atttctaaag cgccatttga
    22621 agagtctgcg cgtaaatatc ctaacgacgc tctggctctt caggcattgt atcgggttat
    22681 taaggaaacg gatttttcta cgccagaaga gatgcgaacg gcgtttccca acctcgacaa
    22741 ttttaaatat cgcaacaaat ggtacgttct ggatgtaggt gggaacaacc taggggtgat
    22801 tgcctacata aactttgtta ataagcggtt ctttgtgaag cacataacta atcacgccga
    22861 gtatgacaaa ctgacacgtt actacagaga gaacaaagaa tgactgctaa tgctgcacac
    22921 gctgttaaag caacaagaga actggtcaat gcagtcccct ttctgggagg tagtgactca
    22981 gaagatgatt atcgtgaagc actggaactg gtcgaatatt tgattgaaga agatgacacc
    23041 aatccactta tcgactttct ggcgagccgc attgcagaat atgaaaacaa caaccaagag
    23101 tttgcagagt tcgacaaagc cgttgcggca atgcctgttg gcgtggcgtt acttcgcacg
    23161 ctgattgatc aacataatct gacctatgcg gatttgaaga acgaaatcgg ctctaaatcc
    23221 ctggtcagtc agattttgtc tggacaacga tccctgacga tatcccacat taaggcactt
    23281 tcagcccgat ttggcgttaa gccggaatgg tttctttgat ttttgactaa tgccaaatgc
    23341 taaattgccc gatgcgctgc gcttatcggg cctacatgtc gcacgaacta tgtaggccgg
    23401 ataaggcgtt cacgccgcat ccggcactgt tacttactct aaatcttccc cattactggc
    23461 aatcactttt ttataccacc agaacgattt cttacgtgtg cgcgtcagcg tgccgttgcc
    23521 tgcgtcgtca cggtctacgt agacaaaacc gtagcgtttg ctcatttcac ccgtagaggc
    23581 ggaaactaaa tcaatacagc cccatgtggt gtagcccatc agcggaatgc catctgcaat
    23641 cgcttcgcct attgcgcgga tatgttcgcg taaatagctg atgcgatagt catcgttaat
    23701 ctcgccattg gcagcaagtt catcttttgc gccgaggccg ttttccacca gaaacagcgg
    23761 cttctgataa cgatcgtaca tcatattcat ggtgatacgc agaccgagtg gatcaattcc
    23821 ccagccccag tcgctcactt gcagatacgg gttacgcagc gatttcacca cgttcgccgc
    23881 actgctgttg ttggcgttca tctccgccga ggcgcagcgc gaggcgtaat agctgaaaga
    23941 gacaaaatcg acggtgtttt tcaggatctc atcatcgccc agcgctttat ctatggttac
    24001 ccctttttcg cgaaatacgc gggcagagta agccggatac gcgccgcgcg cctgcacatc
    24061 gataaaaaac aggttttccc gatctttctc cagcgccgcc cagacatctt ccggtttgca
    24121 gctgtaaggg tagaagttac cgcccgccag catacagccc acctgatttt gcgggttaac
    24181 ctcatgggcg attttggtgg ctagcgcact ggcaaccagc tggtgatgcg cggcctgata
    24241 tttcacctga tcctgatttt caccttcttc aaacaccaga cccgcgccgg agaacgggct
    24301 atgcaacata atgttgattt cattgaaggt tagccagtat ttcaccagac catcaaatgc
    24361 ttcaaagcag gttctggcgt agcggctgaa aaactccacc agcttgcggt tacgccagga
    24421 gccatattcg gtgaccagat gcatcggcac atcgaagtgg cacaacgtga ccagcggttc
    24481 gataccgtac tttttacact cttcaaagac aggacgataa aaagcaatgc cctgctgatt
    24541 gggcgtgatt tcatcgtcct gcggaaagag acggctccag gcaattgagg tacggaaaac
    24601 cttgaatccc atctctgcca tcagggcgat atcttcttta taacgatgat aaaaatccgt
    24661 cgcctcatgg ctgggataaa actcgtcatc gcgcaactga aaacgttttt ccagccccag
    24721 tttcaccgcc attcgatgct cgccgtgtgg gatcatatcg acggtggtca gcccttttcc
    24781 gccttcacgg aacgcacctt cagactggtt ggcggcaagc gcgccgcccc ataaaaaacc
    24841 ttctggaaat actgacattt tcatcctcaa ttaagactta cttctttggc gacggtcggt
    24901 tgatgctttc gcgcctgggc agccgcttcc tcaacaggaa tatcctcaaa gccgagcaac
    24961 agtgtgagga taaacgacag caccaccgcc agcgccatga ccgcgaacac ccagacgatg
    25021 ctcattggat tcgccggatc gaagaattgc acgctggtaa atagccccgg cgctgccatt
    25081 gagtggctgg caagccccgc cataccggca accgcgccgc aaataaaacc gctgataaga
    25141 ctggcgataa gcggacgttt caggcggatc gccacgccgt ataacgccgg ttcggaaatc
    25201 cccgccataa tggctgatgc cgccgcagcc agcgccgtct ggcgcagttc cgggtttttc
    25261 gttttccacg gcaccgccag tgatgaaccg cccagcgaca ggttagcgcc gatctctgat
    25321 ggcatgacca ttccttcttt gccggtttcg gcaatggtct gaatgatggt tggcgtaaag
    25381 acgcggtgca tcccggtcat taccagcaga ggccacagcg cgcccataat ggcgactgaa
    25441 agccagccca gataaccatg aatggtgtaa accagcgccg aaatggcgct accgatccag
    25501 ataccaatcg ggccaatcag caggattgcc agcggtgcgg caatcaacac aatcagcatt
    25561 ggcttgagga agtttttggt caccgccggt gtaatgcgat ccacccagcg ttcgatatat
    25621 gacaggcacc aggtcatcac cagcgccggg atcaccgtgt aggtgtattt caccgcggtg
    25681 accgggatca gggcaaattc gacatgttca ccctgggccg ctttcgccat taattcaata
    25741 aaactcggat gtaccagcac acccgcgatg gcaatcgcca gcgacatatt ggttttaaat
    25801 ttgatggcgg cagatgccgc gaccattagc ggcaggaaga agaaagcacc gtcaccaatc
    25861 acattcagaa tggttaaggt cggcgatcct tttgtcagca cgccgctcat ctcgaggatc
    25921 atcgccagca gtttgaccat cgatccgccg ataatcgccg ggatcagcgg tgacatggtg
    25981 ccgatcagcg catcgaggat ccccgcacca atgcgacgca gcgtgagttt gggtttaccc
    26041 acgggctgtg cgggctgcat atctcccggc agcaggctga cgacttcctg aaaggcctgc
    26101 gaaaccgtat tgccgataat tacctgacac tggttgtcac tacgtaccac gccgagcacg
    26161 ccggggatgg tttttaacgt cgggctgtcg ataagctggt catctttgat aacaaagcgc
    26221 aaacgcgtca tacagtgcgt gactgccgag atgttatcaa cgccgcccag tgccgctatc
    26281 accgagcgtg ccagcgccgc ataatttttg gccatcggat tttatcctgt tatcagtagg
    26341 gtagatgtag ttgttatcag tcgacgtgac ggaaaaatac cgatgctgaa acggaattaa
    26401 gaaaccggtt tcacaaaatc atgaatagtt gtcactaaat aagcaagaga gttaattgtt
    26461 cattttttat atttgtgaaa ccggtcacct gaatattttt cccgccgatt gcgcaaggac
    26521 aatcggcaaa cggtgcagta cactgcgttg attgcgtgta aagggatgaa aatgatgacg
    26581 acgatgctgg aagtggcgaa gcgcgccggg gtttcaaaag cgaccgtttc ccgcgtgctt
    26641 tcaggtaatg gctacgtcag ccaggagact aaagatcgcg tgtttcaggc ggtagaagag
    26701 agcagttacc gcccaaattt gctggcccgc aatctgtcgg cgaagagtac tcagacgctg
    26761 gggctggtag tgaccaacac gctttatcac ggcatttatt ttagtgaatt actgtttcat
    26821 gccgcgcgaa tggcggaaga gaaagggcga cagttgctat tggcagatgg taaacacagc
    26881 gccgaagaag agcgccaggc gattcagtat ctgctggatt tgcgctgcga cgccattatg
    26941 atttacccgc gttttttaag cgtggatgag attgatgaca tcattgacgc gcacagtcag
    27001 ccgataatgg tgcttaatcg ccgcctgcgc aataatagca gccatagcgt ctggtgcgat
    27061 cataaacaga ccagctttaa cgccgtggca gagttgataa acgccgggca tcaggagatt
    27121 gctttcctta ccggctcgat ggattccccc accagcattg aacgtcttgc cgggtataaa
    27181 gacgcgctgt cgcagcatgg tattgcgctc aatgaaaaac ttatcgctaa cggtaaatgg
    27241 acgcccgcca gcggggccga aggggtagaa acgttgctcg aacgtggggc taaatttagc
    27301 gcgttagttg ccagtaacga cgatatggcg ataggtgcga tcaaagcgtt gcacgagcgc
    27361 ggcgtagcgg tgccagagca ggtgtcagtt atcggattcg atgatatcgc aattgccccc
    27421 tatatcgttc cggcgctctc cagcgtaaaa attccggtga ctgagatgat tcaggaaatt
    27481 attggacggc tgatttttat gctcgatggc ggggatttct caccgcctaa aacctttagc
    27541 ggaaaactga tccgccgcga ctcactcatt gccctttcgc gataaacatt gtcatcacat
    27601 gtcatcgtca ctagtgtcga tgacatcttt tttcctgcat tacagaaatt aatttcctta
    27661 aataacagca aattaaaaac tggcatgatt tgtgaatgta tcggcg
//
LOCUS       DADMGC010000044        27536 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.044, whole genome shotgun sequence.
ACCESSION   DADMGC010000044 DADMGC010000000
VERSION     DADMGC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.044"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="JNZ73_001847"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="JNZ73_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HAY8732145.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1958)
                     /locus_tag="JNZ73_001848"
     CDS             complement(1614..1958)
                     /locus_tag="JNZ73_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HAY8732146.1"
                     /translation="MFFGGHVLRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAI
                     LSAAGNEYSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGA
                     STGYTVWQLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JNZ73_001849"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JNZ73_001849"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HAY8732147.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JNZ73_001850"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JNZ73_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HAY8732148.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLHDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /locus_tag="JNZ73_001851"
     CDS             complement(6144..6842)
                     /locus_tag="JNZ73_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HAY8732149.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JNZ73_001852"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JNZ73_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HAY8732150.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="JNZ73_001853"
     CDS             7537..8574
                     /locus_tag="JNZ73_001853"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY8732151.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JNZ73_001854"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JNZ73_001854"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAY8732152.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="JNZ73_001855"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="JNZ73_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HAY8732153.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JNZ73_001856"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JNZ73_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HAY8732154.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="JNZ73_001857"
     CDS             10745..11626
                     /locus_tag="JNZ73_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8732155.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="JNZ73_001858"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="JNZ73_001858"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HAY8732156.1"
                     /translation="MTVTTFSELELDKSLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="JNZ73_001859"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="JNZ73_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HAY8732157.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="JNZ73_001860"
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="JNZ73_001860"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HAY8732158.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLISGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="JNZ73_001861"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="JNZ73_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HAY8732159.1"
                     /translation="MLRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="JNZ73_001862"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="JNZ73_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HAY8732160.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIEFLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="JNZ73_001863"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="JNZ73_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HAY8732161.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="JNZ73_001864"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="JNZ73_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HAY8732162.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTLTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="JNZ73_001865"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="JNZ73_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HAY8732163.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="JNZ73_001866"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="JNZ73_001866"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAY8732164.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="JNZ73_001867"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="JNZ73_001867"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAY8732165.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQPPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="JNZ73_001868"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="JNZ73_001868"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAY8732166.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="JNZ73_001869"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="JNZ73_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAY8732167.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDIQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="JNZ73_001870"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="JNZ73_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAY8732168.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="JNZ73_001871"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="JNZ73_001871"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HAY8732169.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="JNZ73_001872"
     CDS             24893..25273
                     /locus_tag="JNZ73_001872"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAY8732170.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="JNZ73_001873"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="JNZ73_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HAY8732171.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="JNZ73_001874"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="JNZ73_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HAY8732172.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..>27536
                     /locus_tag="JNZ73_001875"
     CDS             26442..>27536
                     /locus_tag="JNZ73_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAY8732173.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSFRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVYEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAV"
BASE COUNT         6792 a         6857 c         7221 g         6666 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tatttaccga aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccgctcc agctatcgtt agccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagcgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgtgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc caggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttggtacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggcacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcact ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgacgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattaa aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagagc ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca tcatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggcttttg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccaccg caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccattgc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggcgatcgca
    15241 aataccagcc ccggaaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatctact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatgc tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagttcc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgctaac ttggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgaggg cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtacgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag ccgccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcttg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggtta tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatac aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgcc actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatga caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac gactatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cattaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttcacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggagtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgctatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgacct caaagccaaa gtagcccggc agaagctgag cccggacagt ttcacaacct
    26881 acggggtgcg tatcgaccgg attaaagcaa cgttcggcga gcgtgaggtg ttcagcgacg
    26941 taacatacaa tcggctggtg gaagtgctgg acgagtggat cgccactcgc tcgaacaata
    27001 acgccctgga gttgtttgcc gagctccgtc ggttctggaa gttctgcgca cctactcttt
    27061 gcaacggccg caatgttgcc gccagtctgc cagatgatta tgtttccttc cgcgtacaga
    27121 aacctacccc cacacggctt tttaccgata ttgaatcaat cgcccgactc tggctcaatg
    27181 ttgctgcctg cacctctgta caccagaaga atgctgttcg cttcatgatc atcactggtg
    27241 ttcgtccgat taatgtccat aacctgcgct gggactacgt ttacgaggag gctggtgaaa
    27301 ttgtttatcc ggaaggggtt atcggcatgc gaggggctat gaaaacacaa aaggctttcc
    27361 gcctgccgat aacgcctgag atccggcgga ttatcgacga gcagaaagcc tggcgtgatt
    27421 cagttcctga gtgcaacagg gattatgtat ttttgcagcc acgtgatcca atgcagccat
    27481 tttcaaaacg atcactggat aagctggtga aaacatacag cccggacggg gctgta
//
LOCUS       DADMGC010000045        27153 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.045, whole genome shotgun sequence.
ACCESSION   DADMGC010000045 DADMGC010000000
VERSION     DADMGC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.045"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..437
                     /locus_tag="JNZ73_001876"
     CDS             <1..437
                     /locus_tag="JNZ73_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HAY8732174.1"
                     /translation="IIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAPAPQV
                     ELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVETLAWF
                     TVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(499..1119)
                     /gene="rhtB"
                     /locus_tag="JNZ73_001877"
     CDS             complement(499..1119)
                     /gene="rhtB"
                     /locus_tag="JNZ73_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HAY8732175.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            1230..2252
                     /gene="pldB"
                     /locus_tag="JNZ73_001878"
     CDS             1230..2252
                     /gene="pldB"
                     /locus_tag="JNZ73_001878"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HAY8732176.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            2260..3060
                     /gene="yigL"
                     /locus_tag="JNZ73_001879"
     CDS             2260..3060
                     /gene="yigL"
                     /locus_tag="JNZ73_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HAY8732177.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            3136..4035
                     /gene="bioP"
                     /locus_tag="JNZ73_001880"
     CDS             3136..4035
                     /gene="bioP"
                     /locus_tag="JNZ73_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HAY8732178.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(3923..4876)
                     /gene="metR"
                     /locus_tag="JNZ73_001881"
     CDS             complement(3923..4876)
                     /gene="metR"
                     /locus_tag="JNZ73_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HAY8732179.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            5112..7373
                     /gene="metE"
                     /locus_tag="JNZ73_001882"
     CDS             5112..7373
                     /gene="metE"
                     /locus_tag="JNZ73_001882"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAY8732180.1"
                     /translation="MTILNHTLGFPRVGLRRELKKVQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(7412..8227)
                     /locus_tag="JNZ73_001883"
     CDS             complement(7412..8227)
                     /locus_tag="JNZ73_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY8732181.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            8489..9250
                     /gene="udp"
                     /locus_tag="JNZ73_001884"
     CDS             8489..9250
                     /gene="udp"
                     /locus_tag="JNZ73_001884"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HAY8732182.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            9391..10818
                     /gene="rmuC"
                     /locus_tag="JNZ73_001885"
     CDS             9391..10818
                     /gene="rmuC"
                     /locus_tag="JNZ73_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HAY8732183.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFCRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPGQPNDEAYQRDDEYNQQSC"
     gene            10913..11668
                     /gene="ubiE"
                     /locus_tag="JNZ73_001886"
     CDS             10913..11668
                     /gene="ubiE"
                     /locus_tag="JNZ73_001886"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HAY8732184.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            11682..12287
                     /gene="ubiJ"
                     /locus_tag="JNZ73_001887"
     CDS             11682..12287
                     /gene="ubiJ"
                     /locus_tag="JNZ73_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HAY8732185.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGIRKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            12284..13924
                     /gene="ubiB"
                     /locus_tag="JNZ73_001888"
     CDS             12284..13924
                     /gene="ubiB"
                     /locus_tag="JNZ73_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HAY8732186.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            14003..14272
                     /gene="tatA"
                     /locus_tag="JNZ73_001889"
     CDS             14003..14272
                     /gene="tatA"
                     /locus_tag="JNZ73_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HAY8732187.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTISDKQADTNQEQAKTEDAKRHDKEQV"
     gene            14276..14791
                     /gene="tatB"
                     /locus_tag="JNZ73_001890"
     CDS             14276..14791
                     /gene="tatB"
                     /locus_tag="JNZ73_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HAY8732188.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            14794..15570
                     /gene="tatC"
                     /locus_tag="JNZ73_001891"
     CDS             14794..15570
                     /gene="tatC"
                     /locus_tag="JNZ73_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HAY8732189.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            15612..16394
                     /gene="tatD"
                     /locus_tag="JNZ73_001892"
     CDS             15612..16394
                     /gene="tatD"
                     /locus_tag="JNZ73_001892"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HAY8732190.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(16391..16879)
                     /gene="rfaH"
                     /locus_tag="JNZ73_001893"
     CDS             complement(16391..16879)
                     /gene="rfaH"
                     /locus_tag="JNZ73_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HAY8732191.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            17046..18539
                     /gene="ubiD"
                     /locus_tag="JNZ73_001894"
     CDS             17046..18539
                     /gene="ubiD"
                     /locus_tag="JNZ73_001894"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HAY8732192.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVTYIDAIWDELAIFNNGKSA"
     gene            18585..19286
                     /gene="fre"
                     /locus_tag="JNZ73_001895"
     CDS             18585..19286
                     /gene="fre"
                     /locus_tag="JNZ73_001895"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HAY8732193.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(19379..20542)
                     /gene="fadA"
                     /locus_tag="JNZ73_001896"
     CDS             complement(19379..20542)
                     /gene="fadA"
                     /locus_tag="JNZ73_001896"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HAY8732194.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARIHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(20552..22741)
                     /gene="fadB"
                     /locus_tag="JNZ73_001897"
     CDS             complement(20552..22741)
                     /gene="fadB"
                     /locus_tag="JNZ73_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HAY8732195.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEITAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            22931..24262
                     /gene="pepQ"
                     /locus_tag="JNZ73_001898"
     CDS             22931..24262
                     /gene="pepQ"
                     /locus_tag="JNZ73_001898"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HAY8732196.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVITLPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLGLIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            24262..24876
                     /locus_tag="JNZ73_001899"
     CDS             24262..24876
                     /locus_tag="JNZ73_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HAY8732197.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            24915..26366
                     /gene="trkH"
                     /locus_tag="JNZ73_001900"
     CDS             24915..26366
                     /gene="trkH"
                     /locus_tag="JNZ73_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HAY8732198.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPAINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            26378..26923
                     /gene="hemG"
                     /locus_tag="JNZ73_001901"
     CDS             26378..26923
                     /gene="hemG"
                     /locus_tag="JNZ73_001901"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HAY8732199.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         6238 a         6911 c         7479 g         6525 t
ORIGIN      
        1 tgattatcga aaaaatggcc tggctgcata cgctgattat ggtgggcggt ggcctgtatc
       61 tctgctggat gggttaccag atgctacgcg gtgcactgaa aaaagaggct gtatctgcgc
      121 ctgcaccaca ggttgagctg gcgaaaagcg ggcgcagttt cctgaaaggc ttactgacca
      181 atctcgccaa tccgaaagcg attatctatt ttggttcggt attctcattg tttgtcggcg
      241 ataacgttgg cactaccgag cgctggggca ttttcgcgtt gattattgtc gaaacactgg
      301 cgtggtttac cgtcgttgcc agtctgtttg ccctgccgca aatgcgccgt ggttatcaac
      361 gtctggcgaa gtggattgat ggttttgccg gggcgttatt tgccggattt ggcattcatt
      421 tgattatttc gcggtgatgc cagacgcgtc ttcagagtaa gtcggataag gcgtttacgc
      481 cgcatccgac attatttttc acgcatgcct cgccgatgct aacagcgctc ccaccagcat
      541 aaacaacgag ccgaaaatct tattcagcgc cttcatctgc tttggtcctt taatccatag
      601 agcaatcctt tgagcaaggg tggcgtaacc gatcatcaca ataatatcga ccacaatagt
      661 ggtgacgccg agcacgatat actgcatcag ttgcggctgt tgcggcatga tgaattgcgg
      721 aaatagcgcc gccagaaaca caatactttt gggattggtg agattcacaa aaactgcgcg
      781 ctggaacaaa tgtcgacgcg attgagtaga ggccagcgat ttaagatcaa ttgcgccagc
      841 ggcgcgccac tgctggattc ccagccaaat caagtaagcc gcgcctgccc actttaacac
      901 ttcaaacgca atcactgagc gggaaaatag cgtccccaac cccacgccaa ccagcacaat
      961 atgaatcgcc agtccggtct gaagcccagc aatagacgcc accgcgccgc gataaccgtg
     1021 gttgagcgag gtggtcatag tgttgattgc accagagcct ggcgacaggc ttaaaatgat
     1081 cgatgtcagc aggtaggcaa accaccattc taaggtcatg atgaactccc ggtgtgtcta
     1141 tttttgtgcc acaatacgct actgtcgcag cgttgtgtca ggcacgctta aaaaaacgat
     1201 tttacgtggt ttaagaggca gattacccga tgtttcagca gcaaaaagac tgggaaacaa
     1261 gagaaaacgc gtttgctgct tttaccatgg gaccgctgac tgatttctgg cgtcagcgtg
     1321 atgaagcaga gtttactggt gtggatgaca ttccggtgcg ctttgtccgt tttcgcgcac
     1381 agcaccatga ccgggtggta gtcatctgcc cggggcgtat tgagagctac gtaaaatatg
     1441 cggaactggc ctacgacctg ttccatttgg ggtttgatgt cttaatcatc gaccatcgcg
     1501 ggcagggacg ttccggtcgc ctgttagccg atccgcatct cgggcatgtt aatcgcttta
     1561 atgattatgt tgatgatctg gcggcattct ggcagcagga ggttcagccc ggtccgtggc
     1621 gtaaacgcta tatactggca cattcgatgg gcggtgcgat ctccacatta tttctgcaac
     1681 gccatccagg tgtatgtgac gccattgcgc taactgcgcc aatgtttggg atcgtgattc
     1741 gtatgccgtc atttatggca cggcagatcc tcaactgggc cgaagcgcat ccacgtttcc
     1801 gtgatggcta tgcaataggc accgggcgct ggcgcgcgtt gccgtttgct atcaacgtac
     1861 tgacccacag cagacagcga tatcgacgta acttacgctt ctatgctgat gacccaacga
     1921 ttcgcgtcgg tgggccgacc taccattggg tacgcgaaag tattctggct ggcgaacagg
     1981 tgttagccgg tgcgggtgat gacgccacgc caacgcttct cttacaagct gaagaggaac
     2041 gcgtggtgga taaccgcatg cacgaccgtt tttgtgaact ccgcaccgcc gcgggccatc
     2101 ctgtcgaagg aggacggccg ttggtaataa aaggtgctta ccatgagatc ctttttgaaa
     2161 aggacgcaat gcgctcagtc gcgctccacg ccatcgttga ttttttcaac aggcataact
     2221 cacccagcgg aaaccgctct acagaggttt aaatttctta tgtaccaggt tgttgcgtct
     2281 gatttagatg gcacgttact ttctcccgac catacgttat ccccttacgc caaagaaact
     2341 ctgaagctgc tcaccgcgcg cggcatcaac tttgtgtttg cgaccggtcg tcaccacgtt
     2401 gatgtggggc aaattcgcga taatctggag attaagtctt acatgattac ctccaatggt
     2461 gcgcgcgttc acgatctgga tggcaatctg atttttgctc ataacctgga tcgcgacatt
     2521 gccagcgatc tgtttggcgt agtcaacgac aatccggaca tcattactaa cgtttatcgc
     2581 gacgacgaat ggtttatgaa tcgccatcgc ccggaagaga tgcgcttttt taaagaagcg
     2641 gtgttcaaat atgcgctgta tgagccggga ttactggagc cggaaggcgt cagcaaagtg
     2701 tttttcacct gcgattccca tgaacaactg ctgccgctgg agcaggcgat taacgctcgc
     2761 tggggcgatc gcgtcaacgt cagtttctct accttaacct gtctggaagt gatggcgggc
     2821 ggcgtgtcga aaggccatgc gctggaagca gtggcgaaga aactgggcta cagcctgaag
     2881 gattgtattg cgtttggtga cgggatgaac gacgccgaaa tgctgtcgat ggcggggaaa
     2941 ggctgcatta tgggcagtgc gcaccagcgt ctgaaagatc tgcatccgga gctggaagtt
     3001 attggtacta atgccgacga cgcggtgccg cattatctgc gtaaactcta tttatcgtaa
     3061 tcgttcttta tttggtcagt tgtcaacctg atacttcgct acaatggata ctcgttaatc
     3121 aaagagtttt ccattgtggc gctacttatc atcaccacga ttctgtgggc cttctccttt
     3181 agcttttatg gcgagtacct tgcggggcac gtcgatagct attttgcggt gctggtgcgc
     3241 gttggcttgg cggcactcgt ttttctgccg tttctgcgta cccgtagcaa tagcctgaaa
     3301 acggtcggcc tgtatatgct ggtgggcgcg atgcagctcg gcgtgatgta tatgctgagt
     3361 ttccgcgctt atctctacct gacggtctcc gaattgttgc tgttcaccgt actgacgccg
     3421 ctctacatca cgctgattta tgacatcatg agtaagcgcc gtctgcgctg gggctatgct
     3481 tttagcgcct tgctggcggt gattggtgcc gggattattc gctatgatca ggtcaccgac
     3541 catttctgga ctggcttgtt gctggtgcaa ctctccaata tcacttttgc catcggcatg
     3601 gtgggttaca aacgcctgat ggaaactcgc ccgatgccgc agcataacgc ctttgcgtgg
     3661 ttctatcttg gcgcgttcct ggtggcggta attgcatggt tcttgctggg aaatgcgcag
     3721 aaaatgccgc aaaccacgtt gcaatggggc attctggtgt ttcttggcgt ggtggcttcc
     3781 gggattggct actttatgtg gaactacggc gcgacgcagg tggacgccgg aacgctgggc
     3841 attatgaata atatgcacgt tccggcaggg ctgctggtaa acctggctat ctggcaccaa
     3901 cagccgcact ggccaacgtt tattacaggc gcgctggtga tcctggcctc actgtgggtg
     3961 catcgtaagt gggtcgctcc gcgctcttca caaacggcag atgatcgcag gcgtgattgc
     4021 gcgctgagcg aataaacgct tccgtaactg gctgacgctg ctcgccatcg cgcacggcgg
     4081 cgtacagtcg gctccacaag ccttcgccca gggttttggt caccaccaga ccctggcgct
     4141 caaaactctc tactacccaa tgcggtagcg cggcaatacc catccgcgcg gcaaccattt
     4201 gaatcaataa taaggtgtta tcgacgcttt tcagtgacgg gctgacgcct gccggctgaa
     4261 gaaaatgccg ccagacatcc agtcgactac gctgcaccgg ataaattaat agcgtctcgc
     4321 tggcgagatc ttccggtgta atacgcgttt ttgccgccag tggatggtca ggtgctaaca
     4381 ccaggcgtac ttcataatcg aacatcggcg aataatgcag gccactgcgc ggcaggatat
     4441 cggaggtcat caccagatcc agctcgccct gttgcaacgc gggctgcggg tcaaacgtca
     4501 cgcccgattt aaaatccatc tctacctgcg gccagttctt atggaaattt tctaaagcgg
     4561 gtgtcagcca ctgaatacag ctatggcact caatggcaat gcgcagacgc gtctgctgcg
     4621 gttcattgca ggcttgcagt gcctggctga tttgcggcag cacctggttt gccagttgca
     4681 acaggatttc tccctgcggt gtaaagcgta gcggctggct cttacgcaca aatagccgga
     4741 agccaaggcg ttgttccaga tcgctaaact ggtgagacag ggcggattgc gtctgatgca
     4801 acgttgccgc agcggctgcg agcgagccgc agttccgcaa cgcttgtagc gttttcaggt
     4861 gttttacttc gatcatgaaa gtccttcact tcggcatgaa taatttgcgc ttgaggaata
     4921 tacagtaacc gccaattatg gatgtgtaaa catctggacg gctaaaatcc ttcgtctttt
     4981 aaatttatgg tgcgttggct gcgtttctcc accccggtca cttacttcag taagctcccg
     5041 gcgatgaata aacttgccgc cttccctaaa ttcaaaatcc ataggattta catataatta
     5101 gaggaagaaa aatgacaata ttgaatcaca ccctcggttt ccctcgcgtt ggcctgcgtc
     5161 gcgagctgaa aaaagtgcaa gaaagttatt gggcggggaa ctccacgcgt gaagaactgc
     5221 tgacagtagg gcgtgaactg cgtgctcgtc actgggatca acaaaagcaa gcgggtatcg
     5281 acctgctgcc ggtgggcgat tttgcctggt acgatcatgt actgaccacc agtttgctgc
     5341 tgggtaatgt tccgccacgt catcagaaca aagacggttc ggtagatatc gacaccctgt
     5401 tccgtattgg tcgtggacgt gcgccgactg gcgaacctgc ggcggcagcg gaaatgacca
     5461 aatggtttaa caccaactat cactacatgg tgccggagtt cgttaaaggc caacagttca
     5521 aactgacctg gacgcagctg ctggaggaag tggacgaggc gctggcgctg agccacaacg
     5581 ttaagcctgt gctgctgggg ccggttactt acctgtggct ggggaaagtg aaaggtgaac
     5641 agtttgaccg ccttagcctg ctgaacgaca ttctgccggt ttatcagcaa gtgctggcag
     5701 aactggcgaa acgcggcatc gagtgggtac agattgatga acccgcgctg gtactggaac
     5761 taccacaggc gtggctggac gcatacaaac ccgcttacga cgcgctccag ggacaggtga
     5821 aactgctgct gaccacctat tttgaaggcg taacgccaaa tctcgacacg attactgcgc
     5881 tgcctgttca ggggctgcat gtggaccttg tacatggtaa agatgacgtt gttgaactgc
     5941 acaagcgcct gccttctgac tggctgctgt ctgcgggtct aatcaatggt cgtaacgtct
     6001 ggcgcgccga tcttactgag aaatatgcgc aaattaagga cattgtcggc aaacgtgatt
     6061 tgtgggtggc atcttcctgc tcgttgctgc acagccccat cgacctgagc gtggaaacgc
     6121 gtcttgatgc agaagtgaaa agctggtttg ccttcgccct acaaaaatgc catgaactgg
     6181 cactgctgcg cgatgcgctg aacagtggtg acacggcagc tctggcagag tggagcgccc
     6241 caattcaggc gcgtcgtcac tctactcgtg tacataatcc ggcggtagaa aagcgtctgg
     6301 cggcgatcac cgcccaggac agccagcgtg cgaatgtcta tgaagtgcgt gctgaagccc
     6361 agcgtgcgcg ttttaaactg ccagcgtggc cgaccaccac gattggttcc ttcccgcaaa
     6421 ccacggaaat tcgtaccctg cgtctggatt tcaaaaaggg caatctcgac gccaacaact
     6481 accgcacggg cattgcggaa catatcaagc aggccattgt tgagcaggaa cgtttgggac
     6541 tggatgtgct ggtacatggc gaggccgagc gtaatgacat ggtggaatac tttggcgagc
     6601 acctcgacgg atttgtcttt acgcaaaacg gttgggtaca gagctacggt tcccgctgcg
     6661 tgaagccacc gattgtcatt ggtgacatta gccgcccggc accgattacc gtggagtggg
     6721 cgaagtatgc gcaatcgctg accgacaaac cggtgaaagg gatgctgacg gggccggtga
     6781 ccatactctg ctggtcgttc ccgcgtgaag atgtcagccg tgaaaccatc gccaaacaga
     6841 ttgcgctggc gctgcgtgat gaagtggccg atctggaagc cgctggaatt ggcatcatcc
     6901 agattgacga accggcgctg cgcgaaggtt taccgctgcg tcgtagcgac tgggatgcgt
     6961 atctccagtg gggcgtagag gccttccgta tcaacgccgc cgtggcgaaa gatgacacac
     7021 aaatccacac tcacatgtgt tattgcgagt tcaacgacat catggattcg attgcggcgc
     7081 tggacgcaga cgtcatcacc atcgaaacct cgcgttccga catggagttg ctggagtcgt
     7141 ttgaagagtt tgattatcca aatgaaatcg gtcctggcgt ctatgacatt cactcgccaa
     7201 acgtaccgag cgtggaatgg attgaagcct tgctgaagaa agcggcaaaa cgcattccgg
     7261 cagagcgcct gtgggtcaac ccggactgtg gcctgaaaac gcgcggctgg ccagaaaccc
     7321 gcgcggcact ggcgaacatg gtgcaggcgg cgcagaattt gcgtcggggg taaatccaaa
     7381 ccgggtggta ataccacccg gtcttttctc attacagcga cttcttccca ccatactgct
     7441 taaaccattc cagcatacgc tgccagccat ctttcgcaga ttcggcatga tagctcgggc
     7501 gataatcagc gttgaatgca tgcccggcat ccgggtacac gataatctct gctttcgcat
     7561 tagcagcccg cagcgcctgg cgcatcgttt caacgctctc ctgcggaatg ctgttatcct
     7621 gaccaccata taagccgaga accggtgcgt taagatcggt tgcgatatca acaggttgtt
     7681 tcggtgaatt cagcgacttg tcgcccgtca gtttgccgta ccacgccact gcggctttta
     7741 gctgtggatt atgcgcggca tacagccagg tgatacgtcc accccagcag aatccggtga
     7801 tcattaaacg atgaacatcg ccgccgttgc gggaagccca actggcgaca tgatcgagat
     7861 cggccagcac ctgcgagtca ggcacttttg ctaccagacc gctaagcaac gtggggatat
     7921 cggcaaaatc attcggatcg ccttcgcgga agtaaagttc aggtgcgata gccagatacc
     7981 cctccagcgc cagacggcga caaatgtcgc ggatatgttc atgcacgcca aaaatttcct
     8041 gcactacaat gaccactggc agtgggccat cgctttgctt tggtctggca tggtaagcag
     8101 gcatgttatc cccttgtgaa gggatagagg tgaagcccgc cacaatcgcg tcgtccgggg
     8161 tctgaacgat ggtcgaagcg agaggcgatg cagcaggtgc aaatccagat tgttgtgttg
     8221 ttgccatggt attctccgta cccttataaa aatgttgcgc aatgttaact atagtcagca
     8281 tgcaacaaat cacattgcct gaatcggctc atcttttatg cagtcctgca gaatgaagag
     8341 tgatttatgt gatatgaatc acttttggtg ggtaaattta tgcaacgcat ttgcgtcatg
     8401 gtgatgagta tcacgaaaaa atgttaaacc cttcggtaaa gtgtcttttt gcttcttctg
     8461 actaaaccga ttcacagagg agttgtatat gtccaagtct gatgtttttc atctcggcct
     8521 cactaaaaac gatttacaag gggctacgct tgccatcgtc cctggcgacc cggatcgtgt
     8581 ggaaaagatc gccgcgctga tggataagcc ggttaagctg gcatctcacc gcgaattcac
     8641 tacctggcgt gcagagctgg atggtaaacc tgttatcgtc tgctctaccg gtatcggcgg
     8701 cccgtctacc tctattgctg ttgaagagct ggcacagctg ggcattcgca ccttcctgcg
     8761 tatcggtaca acgggcgcta ttcagccgca tattaatgtg ggtgatgtcc tggttaccac
     8821 ggcgtctgtc cgtctggatg gcgcgagcct gcacttcgca ccgctggaat tcccggctgt
     8881 cgctgatttc gaatgtacga ctgcgctggt tgaagctgcg aaatctattg gcgcgacaac
     8941 tcacgttggc gtgacagttt cttctgatac cttctaccca ggtcaggaac gttacgatac
     9001 ttactctggt cgcgtagttc gtcactttaa aggttctatg gaagagtggc aggcgatggg
     9061 cgtaatgaac tatgaaatgg aatctgcaac tctgctgacc atgtgtgcaa gtcagggcct
     9121 gcgtgccggt atggtagcgg gtgttatcgt taaccgcacc cagcaagaga tcccgaatgc
     9181 tgagacgatg aaacaaactg aaagccatgc ggtgaaaatc gtggtggaag cggcgcgtcg
     9241 tctgctgtaa ttctcttctc ctgtctgaag gccgacgcgt tcggcctttt gtatttttgc
     9301 gtagcgcctc gcaggaaatg cctttccaac tggacgtttg tacagcacaa ttctattttg
     9361 tgcgggtaag ttgttgcgtc aggaggcgtt gtggatttct caatcatggt ttacgcagtt
     9421 attgcgttgg tgggtgtggc aattggctgg ctgtttgcca gttatcaaca tgcgcagcaa
     9481 aaagccgagc aattagctga acgtgaagag atggtcgcgg agttaagcgc ggcaaaacaa
     9541 caaattaccc aaagcgagca ctggcgtgca gagtgtgaat tgctcaataa cgaagtgcgc
     9601 agcctgcaaa gtattaacac ctctctggag gccgatctgc gtgaagtaac cacgcggatg
     9661 gaagccgcac agcaacatgc tgacgataaa attcgccaga tgattaacag cgagcagcgc
     9721 ctcagtgagc agtttgaaaa cctcgccaac cgtatttttg agcacagcaa tcgccgggtt
     9781 gatgagcaaa accgtcagag tctgaacagc ctgttgtcgc cgctacgtga acaactggac
     9841 ggtttctgcc gtcaggttca ggacagcttc ggtaaagaag cacaagaacg ccataccctg
     9901 acccacgaaa ttcgcaatct ccagcaactc aacgcgcaaa tggcccagga agcgatcaac
     9961 ctgacgcgcg cgctgaaagg cgacaataaa acccagggca actggggcga ggtagtattg
    10021 acgcgggtgc tggaggcttc cggtctgcgt gaagggtatg aatatgaaac ccaggtcagc
    10081 atcgaaaatg acgcccgctc gcggatgcag ccggatgtca tcgtgcggct gccgcaggga
    10141 aaagatgtgg tgatcgacgc caaaatgacg ctggtcgcct atgaacgcta ttttaacgcc
    10201 gaagacgact acacccgcga aagcgcgcta caggaacata tcgcgtcggt gcgtaaccat
    10261 atccgtttgc tgggacgcaa agattatcaa cagctgccgg ggctgcgaac tctggattac
    10321 gtgctgatgt ttattcccgt tgaacccgct tttttactgg cgcttgaccg ccagccggag
    10381 ctgatcaccg aagcgttgaa aaacaatatc atgctggtta gcccgactac gctgctggtg
    10441 gcgttgcgta ctattgccaa cctgtggcgt tatgagcatc aaagccgtaa cgcccagcaa
    10501 atcgccgatc gtgccagcaa gctgtacgac aagatgcgtt tgttcatcga tgacatgtcc
    10561 gcgattggtc aaagtctcga caaagcgcag gataactacc gccaggcaat gaaaaaactc
    10621 tcttcaggtc gcggaaatgt gctggcgcag gcagaagcgt ttcgcggttt aggagtagaa
    10681 attaaacgcg agattaatcc ggatttggct gaacaggcgg tgagccagga tgaagagtat
    10741 cgacttcggt cggttccggg gcagccgaat gatgaagctt atcaacgcga tgatgaatat
    10801 aatcagcagt cgtgctagcc cattaggggt agttaagccg ggtagaaatc tagggcatcg
    10861 acgcccaatc tgttacactt ctggaacaat tttttgatga gcaggcattg agatggtgga
    10921 taagtcacaa gaaacgacgc actttggttt tcagaccgtc gcgaaggaac aaaaagcgga
    10981 tatggtcgcc cacgttttcc attccgtggc atcaaaatac gatgtcatga atgatttgat
    11041 gtcatttggt attcatcgtt tgtggaagcg attcacgatt gattgcagcg gcgtacgccg
    11101 tgggcagacc gtgctggatc tggctggtgg caccggcgac ctgacagcga aattctcccg
    11161 cctggtcgga gaaactggca aagtggtcct tgctgatatc aatgaatcca tgctcaaaat
    11221 gggacgcgag aagctgcgta atatcggtgt gattggcaac gttgagtatg ttcaggcgaa
    11281 cgctgaggcg ctgccgttcc cggataacac ctttgattgc atcaccattt cgtttggtct
    11341 gcgtaacgtc accgacaaag ataaagcact gcgttcaatg tatcgcgtgc tgaaacccgg
    11401 cggccgcctg ctggtgcttg agttctcgaa gccaattatc gagccgctga gcaaagccta
    11461 tgatgcatac tccttccatg tgctgccgcg tattggctca ctggtcgcga acgacgccga
    11521 cagctaccgt tatctggcag aatccatccg tatgcatccc gatcaggata ccctgaaagc
    11581 catgatgcag gatgccggat tcgaaagtgt cgactactac aatctgacgg caggggttgt
    11641 ggcgctgcat cgtggttata agttctgaca ggagaccgga aatgcctttt aaacctttag
    11701 tgacggcagg aattgaaagt ctgctcaaca ccttcctgta tcgctcaccc gcgctgaaaa
    11761 cagcccgctc gcgtctgctg ggtaaagtat tgcgcgtgga ggtaaaaggc ttttcgacgt
    11821 cattgattct ggtgttcagc gaacgccagg ttgatgtact gggcgaatgg gcaggcgatg
    11881 ctgactgcac cgttatcgcc tacgccagtg tgttgccgaa acttcgcgat cgccagcagc
    11941 ttaccgcact gattcgcagt ggtgagctgg aagtgcaggg cgatattcag gtggtgcaaa
    12001 acttcgttgc gctggcagat ctggcagagt tcgaccctgc ggaactgctg gccccttata
    12061 ccggtgatat cgccgctgaa ggaatcagaa aagccatgcg cggaggcgca aagttcctgc
    12121 atcacggcat taagcgccag caacgttatg tggcggaagc cattactgaa gagtggcgta
    12181 tggcacccgg tccgcttgaa gtggcctggt ttgcggaaga gacggctgcc gtcgagcgtg
    12241 ctgttgatgc cctgaccaaa cggctggaaa aactggaggc taaatgacgc ctggtgaagt
    12301 acggcgccta tatttcatca ttcgcacttt tttaagctac ggacttgatg aactgatccc
    12361 caaaatgcgt atcaccctgc cgctacggct atggcgatac tcattattct ggatgccaaa
    12421 tcggcataaa gacaaacttt taggtgagcg actacgactg gccctgcaag aactggggcc
    12481 ggtttggatc aagttcgggc aaatgttatc aacccgccgc gatctttttc caccgcatat
    12541 tgccgatcag ctggcgttat tgcaggacaa agttgctccg tttgatggca agctggcgaa
    12601 gcagcagatt gaagctgcaa tgggcggctt gccggtagaa gcgtggtttg acgattttga
    12661 aatcaagccg ctggcttctg cttctatcgc ccaggttcat accgcgcgat tgaaatcgaa
    12721 tggtaaagag gtggtgatta aagtcatccg cccggatatt ttgccggtca ttaaagcgga
    12781 tctgaaactt atctaccgtc tggctcgctg ggtgccgcgt ttgctgccgg atggtcgccg
    12841 tctgcgccca accgaagtgg tgcgcgagta cgaaaagacc ttgattgatg aactgaattt
    12901 gctgcgggaa tctgccaacg ccattcagct tcggcgcaat tttgaagaca gcccgatgct
    12961 ctacatcccg gaagtttacc ctgactattg tagtgaaggg atgatggtga tggagcgcat
    13021 ttacggcatt ccggtgtctg atgttgcggc gctggagaaa aacggcacta acatgaaatt
    13081 gctggcggaa cgcggcgtgc aggtgttctt cactcaggtc tttcgcgaca gctttttcca
    13141 tgccgatatg caccctggca acatcttcgt aagctatgaa cacccggaaa acccgaaata
    13201 tatcggcatt gattgcggga ttgttggctc gctaaacaaa gaagataaac gctatctggc
    13261 agaaaacttt atcgccttct ttaatcgcga ctatcgcaaa gtggcagagc tacacgtcga
    13321 ttctggctgg gtgccaccag ataccaacgt tgaagagttc gaatttgcca ttcgtacggt
    13381 ctgtgaacct atctttgaga aaccgctggc cgaaatttcg tttggacatg tactgttaaa
    13441 tctgtttaat acggcgcgtc gcttcaatat ggaagtgcag ccgcaactgg tgttactcca
    13501 gaaaaccctg ctctatgtcg aaggggtagg acgccagctt tatccgcagc tcgatttatg
    13561 gaaaacagcg aagcctttcc tggagtcgtg gattaaagat caggtcggta ttcctgccct
    13621 ggtgagagca tttaaagaaa aagcgccgtt ctgggtcgaa aaaatgccag aactgcctga
    13681 actggtttac gacagtttgc gccagggcaa gtatttacag cacagtgttg ataagattgc
    13741 ccgcgagctt cagtcaaatc atgtacgtca gggacaatcg cgttattttc tcggaattgg
    13801 cgctacgtta gtattaagtg gcacattctt gttggtcagc cgacctgagt gggggctgat
    13861 gcccggctgg ttaatggcag gtggtctgat cgcctggttt gtcggttggc gcaaaacacg
    13921 ctgatttttt catcgctcaa ggcgggccgt gtaacgtata atgcggcttt gtttaatcat
    13981 catctaccac agaggaacat gtatgggtgg tatcagtatt tggcagttat tgattattgc
    14041 cgtcatcgtt gtactgcttt ttggcaccaa aaagctcggc tccatcggtt ccgatcttgg
    14101 tgcgtcgatc aaaggcttta aaaaagcaat gagcgatgat gaaccaaagc aggataaaac
    14161 cagtcaggat gctgatttta ctgcgaaaac tatctccgat aagcaggcgg atacgaatca
    14221 ggaacaggct aaaacagaag acgcgaagcg ccacgataaa gagcaggtgt aatccgtgtt
    14281 tgatatcggt tttagcgaac tgctattggt gttcatcatc ggcctcgtcg ttctggggcc
    14341 gcaacgactg cctgtggcgg taaaaacggt agcgggctgg attcgcgcgt tgcgttcact
    14401 ggcgacaacg gtgcagaacg aactgaccca ggagttaaaa ctccaggagt ttcaggacag
    14461 tctgaaaaag gttgaaaagg cgagcctcac taacctgacg cccgaactga aagcgtcgat
    14521 ggatgaacta cgccaggccg cggagtcgat gaagcgttcc tacgttgcaa acgatcctga
    14581 aaaggcgagc gatgaagcgc acaccatcca taacccggtg gtgaaagata atgaagctgc
    14641 gcatgagggc gtaacgcctg ccgctgcaca aacgcaggcc agttcgccgg aacagaagcc
    14701 agaaaccacg ccagagccgg tggtaaaacc tgctgcggac gctgaaccga aaaccgctgc
    14761 accttcccct tcgtcgagtg ataaaccgta aacatgtctg tagaagatac tcaaccgctt
    14821 atcacgcatc tgattgagct gcgtaagcgt ctgctgaact gcattatcgc ggtgatcgtg
    14881 atattcctgt gtctggtcta tttcgccaat gacatctatc acctggtatc cgcgccattg
    14941 atcaagcagt tgccgcaagg ttcaacgatg atcgccaccg acgtggcctc gccgttcttt
    15001 acgccgatca agctgacctt tatggtgtcg ctgattctgt cagcgccggt gattctctat
    15061 caggtgtggg catttatcgc cccagcgctg tataagcatg aacgtcgcct ggtggtgccg
    15121 ctgctggttt ccagctctct gctgttttat atcggcatgg cattcgccta ctttgtggtc
    15181 tttccgctgg catttggctt ccttgccaat accgcgccgg aaggggtgca ggtatccacc
    15241 gacatcgcca gctatttaag cttcgttatg gcgctgttta tggcgtttgg tgtctccttt
    15301 gaagtgccgg tggcaattgt gctgctgtgc tggatgggga ttacctcgcc agaagactta
    15361 cgcaaaaaac gcccgtatgt gctggttggt gcattcgttg tcgggatgtt gctgacgccg
    15421 ccggatgtct tctcgcaaac gctattggcg atcccgatgt actgtctgtt tgaaatcggt
    15481 gtcttcttct cacgctttta cgttggtaaa gggcgaaatc gggaagagga aaacgacgct
    15541 gaagcagaaa gcgaaaaaac tgaagaataa attcaaccgc ccgtcagggc ggttgtcata
    15601 tggagtacag gatgtttgat atcggcgtta atttgaccag ttcgcaattt gcgaaagacc
    15661 gtgatgatgt tgtagcgcgc gcttttgacg cgggagttaa tgggctactc atcaccggca
    15721 ctaacctgcg tgaaagccag caggcgcaaa agctggcgcg tcagtattcg tcctgttggt
    15781 caacggcggg cgttcatcct cacgacagca gccagtggca agctgcgact gaagaagcga
    15841 ttattgagct ggccgcgcag ccagaagtgg tggcgattgg tgaatgtggt ctcgacttta
    15901 accgcaactt ttcgacgccg gaagagcagg aacgcgcttt tgttgctcag ctacgcattg
    15961 ccgcagaatt aaacatgccg gtatttatgc actgtcgcga tgcccacgag cggtttatga
    16021 cattgctgga gccgtggctg gataaactgc ctggtgcggt tcttcattgc tttaccggca
    16081 cacgcgaaga gatgcaggcg tgcgtggcgc gtggaattta tatcggcatt accggttggg
    16141 tttgcgatga acgacgcggg ctggagctgc gggaattgtt gccgttgatt ccggcggaga
    16201 aattgctgat cgaaacagat gcgccgtatc tgctcccgcg cgatctcacg ccaaagccat
    16261 catcccggcg caacgagcca gcccatctgc cccatatttt gcaacgtatt gcgcactggc
    16321 gtggagaaga tgccgcatgg ctggctgcca ccacggatgc caatgtcaaa acactgtttg
    16381 ggattgcgtt ttagagtttg cggaactcgg tgttcttcac actgtgctta atctctttat
    16441 taataagatt aagcaatagc atggagcgag cctcaccatc gggttcggtg aaaatggcct
    16501 gaaagccttc gaacgcgcct tcggtaataa tcaccttatc acccggataa gggggggccg
    16561 gatcgacaat gtctttcggt ttatataccg atagctgatg aatcaccgcc gatgggacta
    16621 tcgctggcga cgcgccaaag cgcacaaagt ggctgacgcc acgcgtagcg ctgatggtcg
    16681 tggtatgaat cacttctgga tcaaattcca caaacaggta gttagggaat aacggttcac
    16741 tgactgcggt acgtttaccc cgtacgattt tttccagggt gatcatcgga gcgaggcaat
    16801 taaccgcctg tctctcaagg tgttcctggg cacgttgaag ttgcccgcgc ttgcagtaca
    16861 gtaaatacca ggattgcata atgactctta tccgtttaat cggggcgcaa ggatagcaaa
    16921 agctttacgc taagttaatt atattccccg gtttgcgtta taccgtcaga gttcacgcta
    16981 atttaacaaa tttacagcat tgcaaagatg aacgccgtat aatgggcgca gattaagagg
    17041 ctacaatgga cgccatgaaa tataacgatt tacgcgactt cctgacgctg cttgaacagc
    17101 agggtgagct aaaacgtatc acgctcccgg tggatccgca tctggaaatc actgaaattg
    17161 ctgaccgcac tctgcgtgct ggtgggcctg cgctgttgtt cgaaaaccct aaaggctact
    17221 caatgccggt gctgtgcaac ctgttcggta cgccaaagcg cgtggcgatg ggcatggggc
    17281 aggaagatgt ttcggcgctg cgtgaagttg gtaaattatt ggcgtttctg aaagagccgg
    17341 agccgccaaa aggtttccgc gacctgtttg ataaactgcc gcagtttaag caggtgttaa
    17401 acatgccgac aaagcgactg cgtggtgcgc cctgccaaca aaaaatcgtc tctggcgatg
    17461 acgtcgatct caatcgcatt cccattatga cctgctggcc ggaagatgcc gcgccgctga
    17521 ttacctgggg gctgaccgta acgcgcggcc cgcataaaga gcggcagaat ctgggcattt
    17581 atcgccagca gctgattggt aaaaacaaac tgattatgcg ctggctgtcg catcgcggcg
    17641 gcgcgctgga ttatcaggag tggtgtgcgg cgcatccggg cgaacgtttc ccggtttctg
    17701 tggcgctggg tgccgatcct gccacgattc tcggtgcagt cacccccgtt ccggatacgc
    17761 tttcagagta tgcgtttgcc ggattgctac gcggcaccaa aaccgaagta gtaaagtgta
    17821 tttccaatga cctcgaagtg ccagccagtg ccgaaatcgt cctggaaggg tatatcgatc
    17881 ctggtgagat ggcgccggaa gggccgtatg gtgaccacac aggttactat aatgaagtcg
    17941 atagtttccc ggtgtttacc gtgacgcata ttacccagcg tgaagatgcg atttaccatt
    18001 ccacctatac cgggcgtccg ccagatgagc ccgcggtact gggcgtggcg ttgaacgaag
    18061 tgtttgtacc gattctgcaa aaacagttcc cggaaattgt cgatttttac ctgccgccgg
    18121 aaggctgttc ttatcgtctg gcggtagtga cgatcaaaaa acagtacgcc ggacacgcga
    18181 agcgcgtcat gatgggcgtc tggtcgttct tacgccagtt tatgtacact aaatttgtga
    18241 tcgtttgcga tgatgacgtc aacgcacgcg actggaacga tgtgatttgg gcgattacca
    18301 cccgtatgga cccggcgcgg gatactgttc tggtagaaaa tacgcctatt gattatctgg
    18361 attttgcctc gcctgtctct gggctgggtt caaaaatggg gctggatgcc acgaataaat
    18421 ggccggggga aacccagcgt gaatggggac gtcccatcaa aaaagatcca gatgttgtca
    18481 cgtatattga cgccatctgg gatgaactgg ctatttttaa caacggtaaa agcgcctgat
    18541 gcgcgtttgt tttgccctat ttatcgatcc gacagagaaa gcgcatgaca accttaagct
    18601 gtaaagtgac ctcggtagaa gctatcacgg ataccgtata tcgtgtccgc atcgtgccag
    18661 acgcggcctt ttcttttcgt gctggtcagt atttgatggt agtgatggat gagcgcgaca
    18721 aacgtccgtt ctcaatggcc tcgacgccgg atgaaaaagg gtttatcgag ctgcatattg
    18781 gcgcttctga aatcaacctt tacgcgaaag cagtcatgga ccgcatcctc aaagatcatc
    18841 aaatcgtggt cgacattccc cacggagaag cgtggctgcg cgatgatgaa gagcgtccga
    18901 tgattttgat tgcgggcggc accgggttct cttatgcccg ctcgattttg ctgacagcgt
    18961 tggcgcgtaa cccaaaccgt gatatcacca tttactgggg cgggcgtgaa gagcagcatc
    19021 tgtatgatct ctgcgagctt gaggcgcttt cgttaaagca ccctggtctg caagtggtgc
    19081 cggtggttga acaaccggaa gcaggctggc gtgggcgtac tggtaccgtg ttaacggcgg
    19141 tattgcagga tcatggtacg ctggcagagc atgatatcta tattgccgga cgttttgaga
    19201 tggcgaaaat tgcccgtgac ctgttttgca gtgagcgtaa tgcgcgggaa gatcgcctgt
    19261 ttggcgatgc gtttgcattt atctgagata taaaaaaacc cgcccctgac aggcgggaag
    19321 aacggcaact aaaactttat tcagtggcat ttagatctat gtcgcatccg gcaagtggtt
    19381 aaacccgctc aaacaccgtc gcaataccct gacccagacc gatacacatc gtcgccagac
    19441 caaactgaac gtctttgcgt tccatcagat tcagcagcgt ggtgctgata cgcgcaccgg
    19501 aacaacccag cggatgaccc agcgcgatcg caccaccatt gaggttgatc ttctcgtcaa
    19561 tctgctccat tagcccgaga tctttaatgc acggcaggat ctgcgcggca aaggcttcgt
    19621 tcatctcaaa cacgccgata tcgctggcag aaagccccgc ttttttcagc gccagcttcg
    19681 aggccggaac cgggccgtaa cccataatcg atgggtcaca accaacgacc gccatcgaac
    19741 gcacacgagc gcgcggctta agacctaatt catgggcgcg gctttcactc atcaccagca
    19801 tggcagctgc gccatcggaa agtgcagaag atgtgcccgc cgttaccgta ccgtttactg
    19861 gatcaaacgc cggacgcagc gtggcgaggg cttccacggt ggtttccggg cgaatcactt
    19921 cgtcgtaatt aaactgcttc aggacgccgt cggcatcgtg accaccagtt gggatgattt
    19981 catttttaaa tgcggccgac tgcgtggcgg cccaggcgcg ggcgtgtgac cgcgcggcaa
    20041 aggcatcctg catttcacgg ctgataccgt gtatacgcgc cagcatttct gccgttaagc
    20101 ccatcatgcc cgccgctttg gctacattgc ggctcaggcc ggggtgaaaa tcgacgccgt
    20161 gactcatcgg cacatggccc atatgctcca cgccgccaac cagacatgcc tgcgcatcgc
    20221 cagtcatgat cattcgtgct gcgtcatgca gtgcctgcat ggatgaacca cacaagcgat
    20281 tgacggtaac cgccgggaca gagtgcggca cttctgccag cagcgccgcg ttacgggcga
    20341 tgttaaaacc ctgctccagc gtctgttgca cacaacccca gtaaatatcg tcgagggccg
    20401 ccgcttccag cgccgggtta cgcgccagca ggctacgcat taaatgagcg gagagatctt
    20461 ccgcacgcac gttacgaaaa gcaccgccct tcgaacggcc catcggggtg cgaattgcat
    20521 cgacaatgac aacctgttcc attgtgattc cttaagccgt tttcaggtcg ccaaccggac
    20581 gggctggctc aaccggagga tagtacggtt cgttatgacg cgctttatta cgcagacctt
    20641 ccggcacttc atacagcggg ccgaggtgct gatattgctg tgccatatcg aggtattttg
    20701 cgctaccgag ggtgtccagc cagcggaacg cgccgccgtg gaacggaggg aagcccaggc
    20761 cgtagaccag cgccatatcc gcttccgccg gagtggcgat aatgccctct tccagacagc
    20821 gcaccacttc gttgaccatc gggatcatca tgcgggcgat aatctcttct tcgctgaaat
    20881 cgcgcttcgg ttggctcact tctgccagca ggtcttcaac ggcggcgtct tcttctttct
    20941 tcggcttacc tttgctgtct tctttataac gccagaaacc gaggccgttc ttctgaccaa
    21001 agcggttggc atcaaacagc gcgtcgatgg catcgcggta atctttctgc atccgctgcg
    21061 ggaagcctgc tgccatgaca gcctgagcgt gatgcgcggt atcaatgccc acaacgtcca
    21121 gcagatatgc cgggcccatc ggccagccaa actgtttttc catcactttg tcgatcttgc
    21181 ggaaatccgc gccgtcgcgc agcagctggc tgaaaccggc gaaatacggg aacagcacgc
    21241 ggttaacaaa gaagccgggg cagtcgttaa ccacaatcgg cgtcttgccc atcttgctcg
    21301 cccaggcgac aactttcgcg atggtttcgt cggagctttt ctcgccgcga ataatttcta
    21361 ccaacggcat tcggtggacc gggttaaaga agtgcatccc gcagaagttt tccgggcgtt
    21421 ccagcgcgtt ggccagttcg ctgataggaa tggttgaagt gttagacgcc agcacggtat
    21481 cctggcgtac tttttgttcg gtttctgcca gtacggcttt tttcactttc gggttttcaa
    21541 caaccgcttc taccacaata tccacgcggt caaatccggc gtagtcgagc gttgggtgga
    21601 tcgtggagat cacgccagcc agtttcagac catcgatctt gccgcgctca agctgcttgt
    21661 tcagcagttt cgcggcttcg gtcatgccga gggttaacga cttgtcgttg atatctttca
    21721 tgacaaccgg cacgcctttc cacgcggact ggtaagcgat accgccaccc atgatgcctg
    21781 cgcccagcac agcagcctgt ttcggggttt caacgtcttt ggtgagtttc ttcgctttgc
    21841 cttttacata ttgatcgtta aggaaaatgc cgaccagtgc gcgagcttcg ttggtatgcg
    21901 ccagcgggac aaaacttttg ttttccagat ttaaggcttc ttcacgacca aaacgggccg
    21961 cagcttcaat ggtttttact gcggtgatgg gggccggata atgtttcccc gccgtttgcg
    22021 cgaccattcc tttagcgatg gtgaagctca tggtggcttc aatcttgctc agttttagcg
    22081 gttccagctt cggctgacgt ttcgctttcc agtcgaggtc gccgttaatg gcctggcgta
    22141 aaatcgccat cgccccttca atcagttttt ctgctttgac tacgccatcc accagaccga
    22201 ttttcagcgc ctgatccgcg ccgacatctt taccggcggc agtgatttcc agtgcactgt
    22261 cagcgcccag catacgtggc atacgtacag aaccgccaaa gcccggcatg atgcccagtt
    22321 tggtttccgg cagaccgatg cgcagatccg gcgtcgccag acgataatcg gtcgccagca
    22381 cgcattcgca gccaccaccc agcgcatagc cgttgacggc agcaatggtc ggcaccggca
    22441 gatcttccag gcgattaaac acgctattgg caaagtgcag ccactgactt aactgttctt
    22501 caggaacgag gaacagggac aaaaattcgg tgatatcagc accgacgata aaggctgctt
    22561 tgttcgaacg cagcagcagc ccttttagat ctgattgctg ttccagcacg ccgatggcct
    22621 cgccgaggct ggcgacggtc gcagtgtcga gtttattaac tgaacctggg gcatcaaata
    22681 ccagttcggc aatgccatct tccagccagt caaggtacag ggtgtcgcct ttgtaaagca
    22741 tgtcagtctc ctgaatccgc aaggtgatct ggtcgtacca gatgcatcgg agtgtgtgtt
    22801 ttgtgttaaa aatatgcaaa taaaagatta aagaaatgcc gatctgatca cgctcggcag
    22861 aaatcacgct ctggatgaac gatgtgctaa gatgcggaga cttaaggtca aaaaaacaga
    22921 agggtaaaaa atggaatcac tggcctcgct ctataaaaat catatagcta ccttacaaga
    22981 acggactcgc gatgcgctgg cgcgcttcaa gctggatgca ttacttattc actccggcga
    23041 gctgttcaac gtttttctcg acgatcatcc ctatccgttt aaagtgaacc cgcaattcaa
    23101 agcgtgggtg ccggtaactc aggtgccaaa ctgctggttg ctggtggatg gcgtgaacaa
    23161 gccgaaactg tggttctatc tgccggttga ttactggcac aacgtcgaac cgctgccgac
    23221 ctccttctgg actgaagatg tggaagtgat cacgctgccg aaagccgatg gcattggtag
    23281 cctgctgcct gctgcgcgcg gcaatatcgg ttatatcggt ccggtgccgg aacgtgcgct
    23341 gcaactgggt attgaggcca gcaacatcaa cccgaaaggg gtgatcgact acctgcatta
    23401 ttaccgctcc ttcaaaaccg agtacgaact ggcctgtatg cgtgaagcgc agaaaatggc
    23461 ggtcaatggt catcgtgcgg cagaagaagc gttccgttct ggcatgagcg agttcgatat
    23521 caatattgcc tatctgaccg cgaccggtca tcgtgatacc gacgtacctt acagcaacat
    23581 tgtggcgctt aacgaacacg ctgcggtgtt gcattacacc aaactggacc accaggcacc
    23641 ggaagagatg cgcagcttcc tgctggatgc cggggccgaa tataacggct atgcggctga
    23701 cctgacccgt acctggtcgg caaaaagcga caacgactac gcacagctgg tgaaagacgt
    23761 aaatgatgaa caactggggc tgatcgccac catgaaagca ggcgtcagct atgtggatta
    23821 ccacatccag ttccatcagc gcatcgccaa actgctgcgt aaacatcaaa tcatcaccga
    23881 tatgagtgaa gaagcgatgg tcgaaaacga tctcaccgga ccgtttatgc cgcacggtat
    23941 tggccatccg ctgggcctgc aggtgcatga cgtcgccggt tttatgcagg atgatagcgg
    24001 tacacacctc gcggcaccgg caaaatatcc gtacctgcgc tgcacccgta ttctccagcc
    24061 gggcatggtg ttaaccatcg aaccgggtat ctacttcatt gaatcgcttc tggcaccgtg
    24121 gcgtgaaggg cagttcagca agcacttcaa ctggcagaaa attgaagcac tgaaaccgtt
    24181 cggcggcatt cgtatcgaag acaacgtggt gatccacgaa aacaacgtgg aaaacatgac
    24241 ccgggatctg aaactggcgt gatggaaagc tggttaattc ctgcggcacc ggtcacggtc
    24301 gttgaagaga tcaaaaagag ccgcttcata acgatgttgg cgcataccga tggcgttgag
    24361 gcggcgaaag cgtttgttga atcggtgcgg gcagaacacc ccgatgcccg ccaccattgc
    24421 gtggcgtggg tggcgggtgc gccggatgat tctcaacagc tgggtttctc tgacgacggg
    24481 gagccggcgg gaacggcagg taaaccgatg ctcgcccagc taatgggcag cggcgtcggg
    24541 gaaattaccg ctgtggtagt gcgctactac ggcggcatat tgcttggcac cggtgggtta
    24601 gtgaaagcgt atggcggcgg cgtgaatcag gcgctgcgcc agctaacgac ccaacgcaag
    24661 acgccattaa ccgaatatac tttgcaatgt gaatatagtc agttaaccgg cattgaagcg
    24721 ttgctggggc agtgtgacgg caaaattatc aacagtgatt atcaggcatt cgttctgctg
    24781 cgggtggcgc ttccggcggc gaaagtggct gaattttccg caaagctggc ggattttagc
    24841 cgtggttcat tgcaattgtt agcgattgaa gaataatccc cacttcgttt tgcagaacta
    24901 aggaagcggc agagatgcat tttcgcgcca ttacccgaat cgttggacta ctggtcatct
    24961 tattttcagg gaccatgatt atccctgggc tggtagcact catctaccgg gatggagcgg
    25021 gccgcgcttt tacccagacc ttttttgtcg ccctcgccat tggctctatg ctgtggtggc
    25081 cgaaccgcaa agagaagggc gaactgaaat cccgtgaggg gtttctgata gtggtgctgt
    25141 tttggaccgt gctgggtagt gtcggtgcgc tcccttttat cttctcggaa agcccgaacc
    25201 tcacgattac cgatgcgttt tttgaatctt tctctggcct gaccaccacc ggggccacta
    25261 cgctggtggg gctggattcg ctccctcatg ccatcctctt ttatcgccag atgctgcaat
    25321 ggtttggcgg gatggggatc atcgtgttag cggttgcgat actgcctatc ctcggcgtgg
    25381 gtgggatgca gctctatcgc gcagaaatgc ccggcccgct gaaagataac aaaatgcgcc
    25441 cgcgaattgc ggaaacggcg aaaactctgt ggttgattta tgtcttgctg accgtcgcct
    25501 gtgcgctggc gttgtggttt gccggaatgg atgcctttga tgccatcggc catagctttg
    25561 cgactatcgc tattggcggc ttctcgacac atgatgccag tatcggttat ttcgacagtc
    25621 cggctattaa cactatcatt gctatcttcc tgctgatctc cggctgtaac tacggtctgc
    25681 acttttcact gttaagtggg cgtagtctga aggtttattg gcgcgatccg gaatttcgca
    25741 tgtttatcgg cgtacagttt acgctggtgg ttatttgtac actcgtactg tggtttcata
    25801 atgtctacag ttcggcgctg atgacaatta accaggcgtt tttccaggtg gtatcgatgg
    25861 cgacaaccgc cgggtttacg actgacagca ttgcccgctg gccgctcttt ttgccggtac
    25921 tgcttttatg ttcagctttt atcggcggtt gcgccgggtc aacgggcggt ggcctgaaag
    25981 tgatccgcat cctgctgctg tttaagcagg ggaaccgtga gctgaaacga ctggtgcatc
    26041 cgaacgccgt ctatagcatt aagctgggga atcgcgcact gccggaacgt atcctcgaag
    26101 ccgtttgggg atttttctcc gcctatgcat tggtgtttat tgtcagtatg ctggcgatta
    26161 tcgccacggg cgtggatgat ttttctgcct ttgcctcagt tgttgcgaca ttgaataacc
    26221 tggggccagg gcttggcgtg gttgctgata actttaccag tatgaacccg gtggctaaat
    26281 ggatcctgat tgccaacatg ctgtttggtc gtctcgaggt ctttacattg ctggtgctct
    26341 ttaccccgac tttctggcgt gaatgatgga gtaatacgtg aaaacattaa ttcttttctc
    26401 aacaagggac ggacaaacgc gcgagattgc ctcctacctg gcttcggaac tgaaagaact
    26461 ggggatccag acggatgtcg ccaatgtgca ccgcattgaa gaaccacaat gggaaaacta
    26521 tgaccgtgtg gtcattggtg cttctattcg ctatggtcac taccactctg cgttccagga
    26581 atttgtcaaa aaacatgcga cgcgactgaa ttcgatgccg agcgcctttt attccgtaaa
    26641 cctggtggcg cgcaaaccgg agaagcgtac tccacagacc aacagctacg cgcggaagtt
    26701 tctgatgaac tcgcaatggc gtcccgatcg ctgcgcggtc attgccgggg cgctgcgtta
    26761 cccacgttat cgctggtacg accgttttat gctcaagctg attatgaaga tgtcaggcgg
    26821 tgaaacggat acgcgcaaag aagttgtcta taccgattgg gagcaggtgg cgaatttcgc
    26881 ccgagaaatc gcccatttaa ccgacaaacc gacgctgaaa taagcataaa gaataaaaaa
    26941 tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg ccggaataac tccctataat
    27001 gcgccaccac tgacacggaa caacggcaaa cacgccgccg ggtcagcggg gttctcctga
    27061 gaattccgac agagaaagca aaaataaatg cttgactctg tagcgggaaa gcgtattatg
    27121 cacaccccgc gccgctgaga aaaagcgaag cgg
//
LOCUS       DADMGC010000046        26233 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.046, whole genome shotgun sequence.
ACCESSION   DADMGC010000046 DADMGC010000000
VERSION     DADMGC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.046"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..694)
                     /locus_tag="JNZ73_001902"
     CDS             complement(<1..694)
                     /locus_tag="JNZ73_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY8732200.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIFSRLALPLFA
                     LFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLRRHLLMASLL
                     TVLAVGAWLFTIS"
     gene            956..2155
                     /gene="lolC"
                     /locus_tag="JNZ73_001903"
     CDS             956..2155
                     /gene="lolC"
                     /locus_tag="JNZ73_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HAY8732201.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            2148..2849
                     /gene="lolD"
                     /locus_tag="JNZ73_001904"
     CDS             2148..2849
                     /gene="lolD"
                     /locus_tag="JNZ73_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HAY8732202.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            2849..4093
                     /gene="lolE"
                     /locus_tag="JNZ73_001905"
     CDS             2849..4093
                     /gene="lolE"
                     /locus_tag="JNZ73_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HAY8732203.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            4122..5033
                     /gene="nagK"
                     /locus_tag="JNZ73_001906"
     CDS             4122..5033
                     /gene="nagK"
                     /locus_tag="JNZ73_001906"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAY8732204.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWEDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            5049..5870
                     /gene="cobB"
                     /locus_tag="JNZ73_001907"
     CDS             5049..5870
                     /gene="cobB"
                     /locus_tag="JNZ73_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAY8732205.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCHFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(6026..7072)
                     /gene="potD"
                     /locus_tag="JNZ73_001908"
     CDS             complement(6026..7072)
                     /gene="potD"
                     /locus_tag="JNZ73_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HAY8732206.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(7069..7863)
                     /gene="potC"
                     /locus_tag="JNZ73_001909"
     CDS             complement(7069..7863)
                     /gene="potC"
                     /locus_tag="JNZ73_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HAY8732207.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(7860..8717)
                     /gene="potB"
                     /locus_tag="JNZ73_001910"
     CDS             complement(7860..8717)
                     /gene="potB"
                     /locus_tag="JNZ73_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HAY8732208.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(8701..9837)
                     /gene="potA"
                     /locus_tag="JNZ73_001911"
     CDS             complement(8701..9837)
                     /gene="potA"
                     /locus_tag="JNZ73_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HAY8732209.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            10087..11313
                     /gene="pepT"
                     /locus_tag="JNZ73_001912"
     CDS             10087..11313
                     /gene="pepT"
                     /locus_tag="JNZ73_001912"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAY8732210.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTNGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(11362..12483)
                     /locus_tag="JNZ73_001913"
     CDS             complement(11362..12483)
                     /locus_tag="JNZ73_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAY8732211.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            12732..13961
                     /locus_tag="JNZ73_001914"
     CDS             12732..13961
                     /locus_tag="JNZ73_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY8732212.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            14356..14544
                     /locus_tag="JNZ73_001915"
     CDS             14356..14544
                     /locus_tag="JNZ73_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY8732213.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            14602..15348
                     /locus_tag="JNZ73_001916"
     CDS             14602..15348
                     /locus_tag="JNZ73_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732214.1"
                     /translation="MKIEYTPERGRGFVRPGETGKPQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCVCDPKHKHGDDSRYQAGGQCNQSGSVRCHNCEVLSFDEYSLRYFSRAKEHGA
                     NRSDSCSFFLRRLFRAGDVFSVDSSLVIVCACKAMRRSTSHHGADSFYPLGSMPRCCN
                     SLRIIRFIQAARESSPSCCCAFSIFSRKSGSKRNWNGGLPRLSFLCVDTLITPHVMYL
                     CVMTHYTQSTQKAKPRTVGAVTGLLTNNVN"
     gene            15357..15557
                     /locus_tag="JNZ73_001917"
     CDS             15357..15557
                     /locus_tag="JNZ73_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HAY8732215.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSTGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            15547..15768
                     /locus_tag="JNZ73_001918"
     CDS             15547..15768
                     /locus_tag="JNZ73_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732216.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            15761..15981
                     /locus_tag="JNZ73_001919"
                     /pseudo
     CDS             15761..15981
                     /locus_tag="JNZ73_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15983..16216
                     /locus_tag="JNZ73_001920"
     CDS             15983..16216
                     /locus_tag="JNZ73_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732217.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            16222..16521
                     /locus_tag="JNZ73_001921"
     CDS             16222..16521
                     /locus_tag="JNZ73_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY8732218.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            16518..18266
                     /locus_tag="JNZ73_001922"
     CDS             16518..18266
                     /locus_tag="JNZ73_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY8732219.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRPQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            18546..18824
                     /locus_tag="JNZ73_001923"
     CDS             18546..18824
                     /locus_tag="JNZ73_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732220.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            18821..19126
                     /locus_tag="JNZ73_001924"
     CDS             18821..19126
                     /locus_tag="JNZ73_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732221.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            19136..19297
                     /locus_tag="JNZ73_001925"
     CDS             19136..19297
                     /locus_tag="JNZ73_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HAY8732222.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            19361..19663
                     /locus_tag="JNZ73_001926"
     CDS             19361..19663
                     /locus_tag="JNZ73_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707048.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732223.1"
                     /translation="MEHYWLFVTVRHSYYLKQTDTTEENAMPMKFDEILKQRDKYHAD
                     NMETMNITDYRAFLETGALIEKDHHGFVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            19963..21120
                     /locus_tag="JNZ73_001927"
     CDS             19963..21120
                     /locus_tag="JNZ73_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY8732224.1"
                     /translation="MQRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPEDDD"
     gene            21160..21732
                     /locus_tag="JNZ73_001928"
     CDS             21160..21732
                     /locus_tag="JNZ73_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAY8732225.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFSFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPREDNRRWWADLAGV"
     gene            21734..22945
                     /locus_tag="JNZ73_001929"
     CDS             21734..22945
                     /locus_tag="JNZ73_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY8732226.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLSAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            22942..23280
                     /locus_tag="JNZ73_001930"
     CDS             22942..23280
                     /locus_tag="JNZ73_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY8732227.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            23277..23573
                     /locus_tag="JNZ73_001931"
     CDS             23277..23573
                     /locus_tag="JNZ73_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY8732228.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            23573..24013
                     /locus_tag="JNZ73_001932"
     CDS             23573..24013
                     /locus_tag="JNZ73_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY8732229.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            23997..24179
                     /locus_tag="JNZ73_001933"
     CDS             23997..24179
                     /locus_tag="JNZ73_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732230.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            24302..24658
                     /locus_tag="JNZ73_001934"
     CDS             24302..24658
                     /locus_tag="JNZ73_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY8732231.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            24642..>26233
                     /locus_tag="JNZ73_001935"
     CDS             24642..>26233
                     /locus_tag="JNZ73_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY8732232.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYMFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDKNEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQA"
BASE COUNT         6843 a         6334 c         7015 g         6041 t
ORIGIN      
        1 gcgagatagt aaacaaccag gcaccgaccg ccagaacggt aagcaaagaa gccatcagca
       61 gatggcggcg caacggcacc tctttaacac aggtcattat tgttgcgcca aaccatgcgc
      121 caaggctgta ataaggcaaa ttgcgtatca cactgttcat tccccaccac ggcgtgggaa
      181 cgaaattaac cgccacactc agcagtacaa acaaggcgaa tagtggcagc gccagacggc
      241 taaaaatttt acataccacg aaatagacaa ttaacgcata cagataccac aagctggtgc
      301 tggcggtgat catcccgtgc aggaactcac cggtggaatc ggcataagcg gcattggagg
      361 cattgcttaa atcgcgctca ggtgccagcc actgcaccac gccccaaagc gccagcaccc
      421 agaagatgtt ccagatccgt ttatcgagac aatttcccca cggtacgctg tcgatatagc
      481 ggcgaatcaa atagccggat ataaagaaaa aaaccggcat acgaaagggg gcaaggtaaa
      541 gattgaaata gatccagcat ttgctcagga cttccgataa cggatgctga aaagtggtca
      601 tatgcggata aaaggtaatg accgagtgat aaatcaccac cagacaaata cataaccctt
      661 tgatctggtt aatccatagc tctttttgtt tcatcagaac aacacgctct tttttaattt
      721 acatgcggtt gatcctgctg gttatgaccc gaggtgtaat gggtatctgt ctgtattgga
      781 tgatttttca gattaagatc aggcggcaag attgatgata aaacatggca atttagccga
      841 ttgatttacg ggggcttttc agattagccc tgacgatcac ttacagttca gacgtttacc
      901 catcttgctt tcgcttatat actcgtgtct ttgctacagc aaccagacgg atttcatgta
      961 ccaacctgtc gctctattta ttggcttgcg ttacatgcgt gggcgtgcag cggatcgctt
     1021 cggtcgtttc gtctcctggc tttctaccat cggcattacc ctcggggtga tggcgctggt
     1081 cacagtattg tcagtgatga acggctttga gcgcgagctg caaaacaaca tccttggcct
     1141 gatgccacag gcaattctct cttctgagca tggctctctt aacccgcagc aactcccgga
     1201 aacggcagtc aaactggacg gcgttaatcg cgtcgcacct attactaccg gtgatgtggt
     1261 actgcaaagc gcgcgcagcg tggcggtcgg ggtgatgctc ggtatcgacc cggcgcaaaa
     1321 agatccacta acgccgtatc tggtcaatgt gaaacaaact gacctcgagc cggggaaata
     1381 taatgtcatc ctcggcgaac aacttgcctc acagttgggc gtcaatcgcg gtgatcaaat
     1441 ccgcgtgatg gtgccatctg ccagccagtt cacgccgatg gggcgtattc caagtcagcg
     1501 cctgttcaat gtgattggca ctttcgccgc caacagtgaa gtcgatggct atgaaatgct
     1561 ggtgaatatt gaggatgcct cgcgtctgat gcgttatccg gcagccaata ttaccggctg
     1621 gcgtttgtgg ctggatgagc cgctgaaagt tgactcttta agtcagcaaa aactgcctga
     1681 aggcagcaaa tggcaggact ggcgtgaccg taaaggcgag ctgttccagg ccgtacgcat
     1741 ggaaaaaaat atgatgggct tactgctgag cctgattgtc gctgttgcgg cgtttaacat
     1801 tattacctcg ctggggctga tggtgatgga gaagcagggc gaagtcgcga tcctgcaaac
     1861 gcaaggctta actccgcgac aaatcatgat ggtctttatg gtgcaagggg ccagcgccgg
     1921 gattatcggt gcaatcctcg gagcggcgct tggcgcactg cttgccagcc agttaaataa
     1981 tctgatgccg ataatcggcg tcctgcttga tggcgcggcg ctgccggtgg ctatcgaacc
     2041 tttacaggtc attgttattg cgctggtggc gatggctatc gcgctgctgt ctacgcttta
     2101 cccttcatgg cgcgctgccg ccacacaacc cgctgaggct ttacgttatg aataagatcc
     2161 tgttgcaatg cgacaacctg tgcaaacgct atcaggaagg cagtgtgcaa accgatgtgc
     2221 tgcacaatgt cagtttcagc gtgggcgaag gtgaaatgat ggcaattgtc ggtagctctg
     2281 gttccggtaa aagtaccttg ctgcacctgt taggcgggct ggatacgcca acctccggcg
     2341 atgtgatctt taacggtcag ccgatgagca aactgtcttc agcggcgaaa gcagaactgc
     2401 gcaaccagaa gctgggcttt atttatcagt ttcaccacct gctgccggat tttactgccc
     2461 tggaaaacgt ggctatgcca ctgctgattg gcaagaaaaa gcccgctgaa atcaacagcc
     2521 gtgcactgga gatgttaaaa gcggtggggc tggagcatcg tgcgaatcac cgcccatctg
     2581 aactttctgg cggcgaacgc cagcgtgtag cgattgcccg tgcgctggtc aataatccgc
     2641 gtctggtact ggcggatgaa cctaccggta acctcgatgc gcgtaacgcc gacagcatct
     2701 tccagttgct tggagagttg aatcgcttgc agggcaccgc tttcctggtg gtcactcacg
     2761 acctgcaact ggcgaaacgt atgagccgcc aactggaaat gcgtgatgga cgtctgacgg
     2821 cggaactgag cctgatgggg gcggagtaat ggcgatgcct ttatcgttat tgattggcct
     2881 gcgttttagt cgcggacggc gacgcggcgg catggtgtcg ctaatctctg tcatttctac
     2941 cattggcatt gccctcggcg tggcggtatt gatcgtcggc ttaagcgcga tgaacggctt
     3001 tgaacgcgaa ctgaataacc gcattctggc ggtggtgccg catggcgaaa tcgaagcggt
     3061 aaatcagcca tggactaact ggcaggaagc actggataac gtgcagaaag tgccgggtat
     3121 tgccgccgct gcgccgtata tcaatttcac cggtctggtg gaaagtggag cgaatctgcg
     3181 cgcaatccag gtgaagggcg ttaacccaca acaggaacag cgtctgagcg cattaccctc
     3241 gtttgttcag ggggatgcgt ggcgcaattt taaagcgggc gaacagcaaa ttatcatcgg
     3301 caaaggcgtg gcggatgcgc tgaaagtgaa gcagggcgat tgggtgtcga tcatgatccc
     3361 caactcgaat cccgagcata aactgatgca gccaaaacgt gtgcgtttgc acattgccgg
     3421 tattttgcag ttgagtggtc aactcgatca cagttttgcc atgatcccgc tggcggatgc
     3481 ccaacaatat cttgatatgg gttccagcgt gtcaggtatt gcccttaaaa tgacggatgt
     3541 tttcaacgcc aataagctgg tacgcgatgc gggtgaagtg accaacagct atgtttatat
     3601 taaaagttgg attggtactt acggctatat gtatcgcgat atccagatga tccgcgccat
     3661 tatgtatctg gcgatggtac tggtgattgg cgtggcctgt ttcaacatcg tctccacctt
     3721 agtgatggcg gtgaaagaca agagtggcga tatcgcagta ttaagaacgc tgggggcgaa
     3781 agatggttta attcgcgcca tctttgtctg gtatggattg ctggcagggc tgttcggcag
     3841 cctgtgtggg gtggttatcg gcgtagttgt ttcactgcaa cttaccccga ttattgagtg
     3901 gattgaaaag ctgatcggtc atcagttcct ctccagcgat atctatttta ttgacttcct
     3961 gccatcggaa ttgcactggc tggacgtctt ctacgtactg gtcacagcat tgttgctgag
     4021 tcttttggca agttggtatc cggcgcggcg cgccagtaat attgaccctg cgcgagtcct
     4081 tagcggccag taaaggcagt acattaaaac aaggagcggc aatgtattac gggtttgata
     4141 ttggtggaac aaaaattgcg cttggcgtgt ttgatagcgg tcggcagttg cagtgggaaa
     4201 agcgggtgcc gacaccgcgt gacagctatg acgcattttt agatgcagtg tgtgagctgg
     4261 tagctgaagc tgaccaacgt tttggctgta aaggttctgt cggcatcggt attccgggaa
     4321 tgccggaaac agaagatggt aagctgtatg ccgctaatgt ccccgctgcc agcggcaaac
     4381 cgctgcgtgc cgacctgagc gcacgtcttg atcgcgatgt acgccttgat aacgatgcca
     4441 actgttttgc cctttcagaa gcctgggagg atgaatttac gcaatatcca ctggtgatgg
     4501 ggttaattct cggcaccggc gttggcggcg ggctggtttt caacggcaaa ccgattactg
     4561 gtaaaagcta cattaccggc gagtttggcc atatgcgtct gccggttgat gcgttaacca
     4621 tgatggggtt ggatttcccg ttacgccgct gcggctgtgg tcagcatggc tgcattgaaa
     4681 attatctgtc tggtcgcggt tttgcgtggc tgtatcaaca ctattatcat caaccgttgc
     4741 aggctcccga aattattgcg ctttatgatc aaggcgatga gcaggcaagg gcgcacgttg
     4801 agcgttatct ggatttatta gcggtttctc tgggaaatat cctgaccatt gttgaccctg
     4861 acctggtcgt cattggtggt ggcttatcga atttcccggc aatcacaacg caactggcgg
     4921 acaggttgcc ttgtcatctc ttacctgtag ctcgtgttcc gcgcattgaa cgcgcgcgcc
     4981 acggtgatgc gggaggaatg cgtggtgcgg ccttcctaca tctaaccgat taaacaacag
     5041 aggttgctat gctgtcgcgt cggggtcatc ggttaagtcg ttttcgtaaa aataaacgtc
     5101 gcctgcgcga gcgtttgcgt cagcgtattt ttttcagaga taaagtggtg ccggaagcaa
     5161 tggaaaaacc aagagtactc gtactgacag gggcaggaat ttctgcggaa tcaggtattc
     5221 gtacctttcg cgccgcagat ggcctgtggg aagaacatcg ggttgaagat gtggcaacgc
     5281 cggaaggttt cgatcgcgat cctgaactgg tgcaagcgtt ttataacgcc cgtcgtcgac
     5341 agctgcagca gccagaaatt cagcctaacg ccgcgcatct tgcgctggct aaactgcaag
     5401 acgctctcgg cgatcgcttt ttgctggtaa cgcagaatat agacaacctg catgaacgcg
     5461 caggtaatac caatgtcatt catatgcatg gggaattgct gaaagttcgt tgttcgcaaa
     5521 gtggtcaggt tctcgactgg accggagacg ttacgccaga agataaatgc cattgttgcc
     5581 acttcccggc acccttgcgt ccgcacgtcg tttggtttgg cgaaatgcca ctcggcatgg
     5641 atgaaattta tatggcgttg tcgatggcgg atgtctttat tgccattggt acttccgggc
     5701 atgtctatcc ggctgctggg tttgtacacg aagccaaact acatggtgct cacaccgttg
     5761 aactgaatct tgaaccgagt caggttggta atgaatttgc cgagaaatat tacggcccgg
     5821 caagccaggt ggtgccagaa tttgttgaaa agttgctgga gggattataa agcaggaagc
     5881 attgcctgat gcgctacgct tatcaggcct acaagcggac cacaataccg acgaaagagc
     5941 gggataaaac tttactgtcc tgactcatta gccacatcct tgctaactaa aaaacgggcg
     6001 gtaataccac cgtccgcttg ttgaattaac gtcctgcttt cagcttctga taatactctt
     6061 cataaatgct gctggctgcg ccaacgtcat tctgccattc gccattttta atggtttcag
     6121 catccgggta gagcgtctta tcgtttgcca cttctggact taacagctta cgcgccgcaa
     6181 ggtttggcgt tggataaccg atagtttcag caacctgttt tgccacatcc gggcgcagca
     6241 ggaagttgat caatttcagc gcgccttctt tgtttttggc atttgccggg atcgccaggc
     6301 tgtccatcca gaatatgccg ccttctttcg gccacaccac gtcaataggc gtacccgcct
     6361 ggcgtgcaac gaaagcagaa ccgttccaga tcatgccgag gttaacttcg ccttccatgt
     6421 acgggttagc cgggttatcg gagttaaacg ctgcgacgtt tggcatcagt tttttcagct
     6481 cgttatatgc agcttcaatc tctttcggat cggtggtgtt accggagtag cccagcttac
     6541 gcagcgccat ctggaacact tcacgggcat cgtcggtcaa cagcaggctg cctttgtact
     6601 ctggcttcca cagatcggcc cagctggtga cagatttcgg atccactgca tcaccgttaa
     6661 caccaatcgc cgtcgcaccc cagatatacg gaatggagta gtcgttattc gggtcaaaag
     6721 gcttgttgag catgtctgga tcgagattgc tgaagttcgt taacttcgac ttgtcgatct
     6781 tctggatcat cccctcttta cgcattttat cgacgtaata ggttgaagga accaccaggt
     6841 cataggcacc gtctttgtac gttttcagct tggcgtacat ggtttcgttc gactcgtaag
     6901 tcgaatagat aaccttaata ccggtttctt tggtgaactg ttcaagcagt cctggcggca
     6961 cgtactcggt ccagttgtag aaatacagcg tgttgttgtc atcggcgtga gcggcgctca
     7021 tgcccagtgc cagagcaccc gccgcgagca ggtggcgtga ccattttttc atttaacgtc
     7081 ccctgtgtta cctttcgttt tatcacgagc aataagctgg ctggcaataa ccatcaccag
     7141 cgacagcacc agtaatatgg ttgccagcgc gttaacttcc ggcgatacgc cgactttgac
     7201 catcgaataa atttttaacg gcagaatttc ataacttggt ccggtgacga atgaagaaac
     7261 taccacatcg tccatcgaca gggtaaagct taacacccag cccgccgcca ccgctggcat
     7321 tgccagtggc agaatgattt ttcgcagaat ggtaaattcg ctggcaccga gatctttcgc
     7381 cgcttccagc atccgcacgt caaaaccttt caggcgcgaa tacaccgtca ccaccacaaa
     7441 tggcaggcag aaggtgatat gcgagaacag cagcgaccag aagccaagct gaatacccag
     7501 cagcataaac agcaccagca gcgaaattgc catcacgata tctggtgaca tcattaccac
     7561 aaacagcatt ccgctaacga acggcttacc gcgaaagcga taacggtaca gcgcaactgc
     7621 cgtcagtgaa ccgataagcg tagcaaacgt cgccgaaaac accgccattg ttagtgaatg
     7681 ctgcgctgcc tgtaacaggc tatcgttgtt catcagcagg ctataccatt tggtggtaaa
     7741 accctgccag ttgatgccaa agcgcgagct gttaaaggag ttcacaatca aaataatgat
     7801 tgggatatac aggtacgcgt agatagcggt cataaaaccg ccgcgaagca gtcgaccgat
     7861 cattcgagtt ccaccttctt attcagcaaa cgagaagcgc gccagtaaac cagcaacatc
     7921 aggcccatta cgatagtcag cgtaatgctg gtggctgcac caaacggcca gtcacgaata
     7981 ttgaggaact gtaccttgat gacgttaccg atcagcaggt ttttcgcacc gcccatcagg
     8041 tcggacacat agaacaggcc catcgctggc agcatcacca gcagacatcc ggcaataatc
     8101 cccggcatcg tcagtggaat aatgatgcgg ataaaagtct gtaacttgct ggcaccgaga
     8161 tcgcgcgccg cctcaagcaa cggcttatcc agtttttcga tactggagta cagcggcatc
     8221 accataaacg gcagcagaat gtaaaccaga ccgataatca ccgcactggg cgtgaacatg
     8281 atacggattg gtgtgtcgat aacgcccagc cagagcaaaa actcgttgag atagcctttg
     8341 gtgctgagga aaattttcag cccgtagata cgaatcaatg agttggtcca gaacggaaca
     8401 atcagcagaa acagcagcag cggacgcacc ttgtgtggca gcttcgccag aaaccaggca
     8461 aatggatagc ccagcaccag gcaggcgagg gtggcgatca gcgccatatt cagcgagtgc
     8521 aatagcactt caaaatagag cggatcgagc agacgcgtgt agttatccag cgtaaagacc
     8581 attttgacga aactggcgtc gtcgcgggtc aaaaagctgg tgccaatgat catcaggttg
     8641 ggcagaaaga caaacaacac aagccaaccg acaatagtga caatcactac attctggaac
     8701 ttacttgtgt tcttcatcag ccagtacgac ctcccagctt tctacccaat taatggccat
     8761 tttttggtcg agagagtggt caaagtcagg atcgtcttca ttgaagaatt cgctgaccat
     8821 caccatcttg ccattttcca gttcaacaac cgactccagc gtcatgcctt tgtagttacg
     8881 ctcacgaacg taaccaatca gcccttcagc gtggttgtcg tcgttaatct cttcaacacg
     8941 taagtcttcc gggcgcagca gaacatgcag tttttgcccc ggttcaacgg cgaagttaac
     9001 gtagatatta cattcgcggc cttcaacgtt ggcgcgtacg cgctgctcgt cgagtcgctc
     9061 gatcaccgtg gcgttaaaca tattaatttc gccaatgaac ccggcaacaa acaggttttt
     9121 cggctcttcg tagatttcac gcggcgtgcc gtcttgctca atgcgaccat cgcgcatcac
     9181 cacaatcctg tccgacatgg tgagtgcttc ttcctggtcg tgagtcacga aaacgaatgt
     9241 aatgccaagc ttacgctgta acgctttcag ctcgttctgc atttgcttac gcagtttgta
     9301 atccagcgct gagagcgact catccagcaa caacagacga ggcttgttaa ccaccgcgcg
     9361 agcaatggcg acgcgttgct gttgaccacc agagagctga tgcggtttac gttgagcgaa
     9421 ggtttccagc tgcaccatcc gcagggcttc catcacgcgg ggcgtaattt cagcagcggg
     9481 ggttttttgc atgcgcaacc caaaggccac attttcgaac acggtcatgt gggggaaaag
     9541 tgcatagctt tggaaaacag tgttcacata gcggttttcc gccggaacgt gggtgatgtc
     9601 ctcgttatcc agcatgatgc gtccggaatc aacagtttcc agacctgcaa tcaggcgaag
     9661 aacggttgtt ttaccgcagc cagaagggcc aagcagcgtg aggaactcgc cattgttgat
     9721 agtcagatcc agctggggaa tgacctcttt accatcaaag catttgcgaa ttcccgccaa
     9781 ttgcaccagc ggtgaaagcg aactcggttg tttattcaat tttttactct gtcccatgta
     9841 aacgcaacgg atggcttacc gatgcggggt ttgtggttaa ccaccttggt gactcttaat
     9901 gagggcggta attctacggc aaaccgcttg aatcgccaat ctttgttgtg aattactggc
     9961 ttagctttat attcattaag gtaatgctga taaatattcc cgcttgcagg ggtaaaagtg
    10021 acctgacgca atatttgtct tttcttgctt cttaataatg ttgtcacaaa aagtgagggt
    10081 gactacatgg ataaactact tgagcgattt ttgaactacg tgtctctgga tacccaatca
    10141 aaagcagggg tgagacaggt tcccagcacg gaaggccaat ggaagttatt gcatctgctg
    10201 aaagagcagc tcgaagaaat ggggcttatc aatgtgacct taagtgagaa gggcactttg
    10261 atggcgacgt taccggctaa cgtccctggc gatatcccgg cgattggctt tatttctcat
    10321 gtggatacct caccggattg cagcggcaaa aatgttaatc cgcaaattgt tgaaaactat
    10381 cgcggtggcg atattgcgct gggtatcggc gatgaagttt tatcaccggt tatgttcccg
    10441 gtgctgcatc agctactggg tcagacgttg attaccaccg atggtaaaac cttgttaggt
    10501 gccgatgaca aagcaggtat tgcagaaatc atgaccgcgc tggcggtatt gcaacagaaa
    10561 aacattccgc atggtgatat tcgcgtcgcc tttaccccgg atgaagaagt gggcaaaggg
    10621 gcgaaacatt ttgatgttga tgccttcgat gcccgctggg cttacactgt tgacggtggt
    10681 ggcgtaggcg aactggagtt tgaaaacttc aacgccgcat cggtcaatat caaaattgtc
    10741 ggtaacaatg ttcatccggg cacggcgaaa ggagtgatgg taaatgcgct gtcgctggcg
    10801 gcacgtattc atgcggaagt tccggcggat gaaagcccgg aaatgacaga aggctatgaa
    10861 ggtttctatc acctggcgag catgaaaggc accgttgaac gggccgatat gcactacatc
    10921 atccgtgatt tcgaccgtaa acagtttgaa gcgcgtaaac gtaaaatgat ggagatcgcc
    10981 aaaaaagtgg gcaaagggtt acatcctgat tgctacattg aattggtgat tgaagacagt
    11041 tactacaata tgcgcgagaa agtggttgag catccgcata ttctcgatat cgcccagcag
    11101 gcgatgcgtg actgcgatat tgaaccggaa ctgaaaccga tccgcggcgg taccaacggc
    11161 gcgcagttgt cgtttatggg attaccttgc ccgaacctgt tcactggcgg ttacaactat
    11221 catggtaagc atgagtttgt gattctggaa ggtatggaaa aagcggtgca ggtgatcgtc
    11281 cgtattgccg agttaacggc gcaacggaag taagcgaaaa gggatgcggc atgtgatgct
    11341 gcatccggct taaatccaaa cttacccttc gaagaaccaa tacccgctat tgaccagcgc
    11401 cgcgagcatc gcgaggaatg acggatcttc cagcgcatcg ccaaaattct ccgcagtcag
    11461 cgcaatgttg ctggcgagtg catccagtgc cggacggtgc ggggaatcga tcttctcacc
    11521 attggcatac acgtcgtcgc caatgcgcaa tacgcgcaga ccacccaggc gcaccagcac
    11581 atcaccttgt ttcagtgcat cgtagatttc atctggctga taaggcggtt ccggcggcgc
    11641 gatatccagt tcatgacgtg actgggatat aaactcgcca aaccattgct taaagtgttc
    11701 cggctggttg atcaattcga gcatcatctc acgcagttta tccatctctt gcggcagaac
    11761 atccgcagga tgagcgcgag gtggaacatc cggatcgctg tagtagttgc cgcccagttc
    11821 acgttgcagc acataatcgg caaatccgct aattaattcc cgcgtatttg gcgcgcgaaa
    11881 acccacggaa tagttcatcg cattttccag cgcgtagcct tcatgcggga atcctggcgg
    11941 aatataaaga atatcaccag gctccagctc ttcatcgatg atggcttcga acggatcgac
    12001 ctgtaacaga tctggatgtg ggcagtgctg tttcatttgc agcttttcgc ccactcgcca
    12061 gcgacgacgt ccggtaccct gaatgataaa cacgtcgtac tgatcgagat gcgggccgac
    12121 gccgccgcca ggtacagaaa aagaaatcat cagatcatca atacgccagt ccggtagttc
    12181 acggaacggt cgcatcagcg cggcggtcgg ctcatgccag tggtttactg cctgtaccag
    12241 taatgaccag ttggtttcac cgagatgatc gtagctttcg aacgggccgt ggctgacctg
    12301 ccatttgcca tcctggtgac tgaccagtcg actgtcaact tcgctttcca tcgccagacc
    12361 cgccaactcg tctggagaga tcgggtcaat aaaattatta aagccgcgtt ttaacaccac
    12421 cgggcgtttc tgccagtgac gttcaagaaa atcgggccag ttaagagtga gttggtattc
    12481 catgttaagc atccacaggc tggtatctgc aaccgattat aacggatgct taacgtaatg
    12541 cgtgaagtat ggacatattt tatcaattag tgttttttgc cgtttcataa tgtttcagat
    12601 tgttcattat gtttttgatt ataaacagat ttgtgatttt ccttgttcca gcttgtttca
    12661 cgttgtagcc ctttgtgcaa cgatcggtgt agttaatggt gtagttaatt gtgatgaccg
    12721 gaatcaaaat tatgagcaga gcacttaaca aactgagcga tacacagctg aggaaaatca
    12781 acggcacacc cgcccaaaaa acagcctttc ttaatgacgg tggaaacctg agcgtcaggc
    12841 attcaaccag tggcctttta acctggtatt tcacttacag ggccggaacg ggaagggggg
    12901 caccaccgga acgcattaag ctgggaaatt atcctgatct gagcctgaaa tcagccaggg
    12961 aaaaagccgc ccagtgtcgc gcatggctgg cagaggggaa aaatccacgt catgagctta
    13021 attacaccgt acaggaagcg ttaaagccgg taacggttgg cgatgcgctc acctactggc
    13081 ttgagtcgta cgcaaaggaa aaccgcgtgg attatgccgc cctgaaaaag cgccttaata
    13141 atcacgtaat acagcacatt ggtgctatgc cgctggataa atgcgagcta cggcactggc
    13201 tggcctgttt tgaccaggtg gcaaagcgaa cgcctgttac tgccggattc ttgctacaga
    13261 cgtgcaaaca ggcgcttaag ttctgccgga ggcggcgcta tgcaatcagc aacgttcttg
    13321 atgatatgag tgtggcggac gttgggaaaa aaccggatat aagcgagcgt gtcttaagca
    13381 ccaaagaact gggcgaatta ttgcaggcac tggacaaaaa aatattctcc ccctactaca
    13441 tcgcgttaat ccgcctcctg attgtgttcg gatgccggac ggtagaactg aggttatcgg
    13501 agatcagcga gtgggatttt accgaaatgc tctggaccgt gccgaaagaa cacagcaaaa
    13561 cgaaggtggc aatattccgg cccataccgg aagcaatact gccgttcgtc acgcagctgg
    13621 tggagcagaa caggcacacg ggcttattgc tgggggaagt gaaacaggaa acaagcgtgt
    13681 cgcagtacgg cagattagcg cacaggaggc taaatcaccc tcactggtca ctacatgaca
    13741 tccggcgcac ctttacaacg atgctgaacg atttaggcgt ggatcctcac gtcgtggagc
    13801 agcttacagg ccaccagatg ccaggaatgc agcgagttta taatcattcc cgttatctgg
    13861 atgctaaacg caatgcgctg gatatgtgga cggagcggtt agggatactg gcgggaacac
    13921 atgaaaacgt aaccacgcta ccagtagcca gaagaaaata atttttttcg tgttttttca
    13981 gtatgcgcat actggatatg cgaacagata caacgtgcaa caatgagcaa caatgagcaa
    14041 caatgagcaa caatgcggaa caactacgaa caagaggtga aaaagtgtac agatttataa
    14101 gtagctgatt tttaacgtct tactggtttt ttatacacta gcgaatcact ctttaaatag
    14161 cgagaaaaaa ggcatgaaac aatattttat gcctttgtat taacatgaat ttaaatgatt
    14221 ttatcctgat tattcctctt tctgtaaacc atacggcctc aacatttgcc aactctgaat
    14281 catcctgaca caatcagaca catcccaacg caatagaaca cgaaataact ctttaagaaa
    14341 cgaaaggggg caatagtgtt aagcactgat cggtttatac gtgaaaaaga atgcgaaaag
    14401 ctaacaggcc ttagccgctc atgccgctac cgcctggaaa aggccggaca attcccatca
    14461 cgtcgtaaac ttggcggtcg ttccgttggc tggtctttat ccgaggttct ggcctggaag
    14521 gatagctgca aggcagttca ttaatcacgc tggcggcacg cagccgccac acatcaatca
    14581 tctgaacaca gagctataac catgaagatt gaatatacgc cagaacgtgg gcggggattc
    14641 gttcgccctg gtgaaactgg aaaaccacaa aattggggtt tttcaggcat aaaaaaagcg
    14701 gccccgaaat ggagccgcct ttctgaacag ataacccgct gcgccgtttg tgtgtgtgat
    14761 cccaaacata agcacgggga tgatagccgc tatcaggctg gtgggcaatg caatcagtct
    14821 ggttcagtgc gttgccataa ctgcgaagtt ctctcctttg atgagtattc tttaaggtac
    14881 ttctcaaggg caaaagaaca cggcgcgaat cgttctgatt catgctcttt ctttctgcgt
    14941 cgtctttttc gtgcaggtga tgttttttcg gttgattctt cgttggtcat tgtgtgcgcc
    15001 tgtaaagcaa tgcgccgtag tacctcacac cacggcgctg atagttttta tcctttgggt
    15061 tctatgccgc gttgctgtaa ttctttgcgg attattcgct ttatccaggc agcaagagaa
    15121 tcatcgccgt cctgttgttg tgccttttcc atcttttccc gtaagtcagg atctaaacgg
    15181 aactggaacg gaggattacc acgcctttcg tttttgtgtg ttgacacgtt aattacacct
    15241 catgtaatgt atttatgtgt aatgacacat tacacacaat caacacaaaa agcaaagccc
    15301 cgcactgtag gagcagtaac ggggcttcta accaacaacg taaactagga gccgttatgg
    15361 ttgccgtaaa tcatatacca caccttgtac acacacaaac ggcctttgtg tggcgttttc
    15421 tggcactgag taccggagaa tcccaaatca tccacgtaac cgcttggacg gaacgcgaag
    15481 cgcgtagccg ttgcccgtcc ggttgtgttg ctgtattcgc cgcccgtatt cgtcaggggg
    15541 aaacctatgc acaataaaac cacaccgaac gcagcagccg ccgcactcac tacgctgatg
    15601 cacgcgctta ttgatattga atgcacggca gagcttgcgc agggagaaga acagaaagac
    15661 cggacacagt tcgccctgga atgtatccga tacatcgcaa cgcggtcact gaatgacgct
    15721 aaaaatattc ttgttgccga ttgtgaaaat ggggggggtt atgcgtgatg atcgttttaa
    15781 ttccctgaaa caggaatttt ccggcgttcc tgatgatgcg gctgatgcgc tttcgtcaat
    15841 gccagaactt attagagcgg cttttttctt actttccacg agagaatata aatcaacggg
    15901 cttgatgtac tgaatatcgc cgccgattat gctgatttcg tgacagaggt tattttaaga
    15961 aaaacgacgg acggggatta atatgcgtga tatttaccac gaaacaatag accgcgcatt
    16021 tcttgcactt tctcacagtg aaaacatgct ggaaatattg cgcatatggc ttgaaacact
    16081 tggcgacaat gaacgcgaca aacaaaaatc aagaattgcc acggcattaa taacgcttct
    16141 tgagcctgta ataatggaac tgcaagaaat agatctattg cacgacagat ataaagaaca
    16201 gcacaccgga gaataaaaat aatgaaactt aaatattctg gcttaactgc cagtggcaac
    16261 actcacccta aatttacgcg cggtgatatt taccgcgacc agtacggcgg catggtaacg
    16321 attaaaggcg tggcagaacg gcgcatcacc taccgccgtg aaggttacga atatgattgc
    16381 gtgatgcctg tttatcaatt ccggcgtgat ttcacgctgg tacaggcagc gccccgcagt
    16441 aaaccaacca gcagggagaa agcacgcgcc aatattcagg aaataaaaaa gatgcttaac
    16501 gtattcaggg gtaaaaaatg aaactggcac cgaacgtaaa aaaacagcca cgcggaataa
    16561 aacacaaaga cacagaggta attatctttg cgggtagtga tgcctggtcg cacgcgaaac
    16621 aatggcagga gcaggatggc cccgcatccg gcgataatgt gccgcctgtg tggcttgggc
    16681 caaatcagct tgccgaactt gatgcactga aaattgttcc ggatggaaaa aaacgcgtaa
    16741 ggctgtacca ggccggagaa ctggatttgg tggagaccaa aaagattggt cagaagctgg
    16801 cggcggcaga tattcaggac gcaaattttt accccgaagg aatgcacgtc cagaagtgtg
    16861 aaaactggcg gcgctatctg aatgctgagc gtgaaaatat tgccgcaggg cttaccatgc
    16921 cggagcagaa aaatacgcaa ctggcacaaa tggcagacag tgagcgcgcg cagctgcttg
    16981 ccgagcgctt tgatggcgtt tgtgtgcatc aggaaagtga aatcgttcac gtatggcgcg
    17041 gcggggtatg gtgtccggtc agcacaatgg aacttagccg cgaaatggtg gcgatctatt
    17101 cagagcacag ggccactttc agcaagcgcg taatcaataa cgccgtggaa gcgttaaaag
    17161 ttattgccga accaatgggc gagccgtccg gcgatttgct gccgttcgcc aatggtgcgc
    17221 ttgacctgaa aacgggggaa ttttccccgc acacgccgga gaactggatc accacgcaca
    17281 acggcattga gtacacgcca ccagcaccag gggagaacat ccgcgataac gcgctaaact
    17341 ttcataaatg gcttgagcac gcagccggaa aagaccagcg caagatgatg cgtatatgtg
    17401 ccgcgctgta catgattatg gcgaaccggt acgactggca gatgtttatt gaggccaccg
    17461 gagacggcgg gagcggtaaa agtacattca cacacatagc cagccttctg gcagggaaac
    17521 agaacacggt aagcgctgaa atgacatcgc ttgatgatgc tggtggacgt ccgcaggttg
    17581 tcgggagtcg tcttatcgtc ctggctgacc agccgaaata tacaggcgaa ggaacgggca
    17641 tcaagaaaat cacgggcggt gaccccgtgg aaattaaccc gaaatacgaa aagcgtttca
    17701 cggctgtaat cagggcggtg gtgctggcga ccaacaataa cccgatgata ttcaccgaac
    17761 gggccggagg tgtggcacgt cgtcgcgtga ttttccgttt cgacaatatc gttagtgagg
    17821 ccgaaaaaga cagggagcta ccggaaaaga ttgcggctga aatccctgtc attatccgcc
    17881 gattgctggc gaactttacc gactctgaga aagcacgggc tttactactg gaacagcgtg
    17941 acggtgatga agctctggca ataaagcaac agacggatcc ggttattgag ttttgccagt
    18001 tcctgaattt tctggaggaa gcacgcggcc tgatgatggg cggcggtggc gattcagtga
    18061 agtacacgac cagaaacagc ctttaccgcg tctatctggc gtttatggcg tacgcaggca
    18121 ggagcaaacc gctaaacgta aatgactttg gcaaggctat gaagccagcc gcgaaagttt
    18181 acggacatga atatattacg cggaaagtta agggagtaac gcagactaac gcaataacaa
    18241 cagacgagtg cgacgcgttt ttataatttt ttgtaattgc tgtctaccct atctacctga
    18301 gtaaagaaaa atacatttaa ttcagtgcat taagttaggt aggtagcctt tttttactgt
    18361 ctacccacta tctaccctct ctacctgatt ttacctgaat cagacaggga ggtagacacg
    18421 gggtagatag tggataaaag cactctaccc cgctgaaagc agcgccatta ctggcatgag
    18481 ggccactaag gtagacaagg ggaggcacaa ctcaaaactt tttaaacgag ggggtaaaaa
    18541 taaatatgca cacttcaggg aaatttaata aatcactcaa aaaacgcaga gacagaacag
    18601 aaccgaaata tcgcgcgtta gacatgacag agcacgcttt aaaggtggca atcagaacga
    18661 tagaccgcca cgcgggggaa ggatacgcga aagcacatcc cgaactgata agcgcattca
    18721 tgaccacgac ggcggcaaat tttgccacgc tgacagagcg ggagattgcc gaagcggaac
    18781 aggtaacaac aatcaacgtt aaaaccggag aggtggaatc atgagcgaac caaaatttgg
    18841 tgaaaaattt tacaagcaca acggacggat cacgattctg caaataagcg cagctacacc
    18901 aggctggtgg gttgaaacag acgaaggatc ctcacccgtg gcatcatggg cgctttgtgt
    18961 gcttagttac cctgaccgcg atgtgtatca ggatattctg cctgtgatat ccactgacaa
    19021 aggcatgaaa cccgtagaca tcaaaaacat ggggtttcag tgcgtaatgc tcactgaaaa
    19081 aatgatggaa gagatgaaaa agaacaatgc cggatcgctt cactgaggtg gcaatatgac
    19141 agcacaaatt gcagcttacg ggcggctggt gggcgacccg ctggtaaaac agagcagcaa
    19201 gggcacaccg gagccgccaa cggagaacta cgcagacctg agaaatgcag taaatcgcac
    19261 ttacgcagac atggggctga gcaaattagc agagtaggaa tatcagaacc acaccacgaa
    19321 gccggagcaa tccggctttt tttgcgtcat ttgaaacggt atggagcact actggctttt
    19381 cgtcacggtc cggcatagtt actatctgaa acaaacagac acaacagagg aaaacgcaat
    19441 gccgatgaaa tttgatgaga tacttaaaca gcgtgataaa taccatgctg acaacatgga
    19501 gacgatgaat atcactgatt accgcgcatt tctggaaacg ggggcgctga ttgaaaagga
    19561 tcatcatggt tttgttcgtt gcgcgctatc cggtgaaatg ctggcagtaa atcctgaaca
    19621 gacagatgca ttgatagaat ttctgaaagg gatcagagac tgagcaccca tacagcacat
    19681 acagccggag caatccggct ttttgttatt ttttgtaaat tatttgttcg tggttgttcc
    19741 acgttgttca ctgagcggat cggcatattt tacccgaact gaatcatgat tattctcgcc
    19801 cgtggtgcca ggacgctggg gccactttcc cgcctgttaa tgtgctcgcc aatattcatt
    19861 accaggcggg aaaacgatcg gtgcgattac tgatttcctt atgaaaaacg gttgagtgtt
    19921 tgccgcgtcc tggagttcct tacttaaccc caggactttt ttatgcagag aataatcgaa
    19981 ttacgccagc agaaaaccgc cattaaaaat cagatgcgcg acatgctgga gaacgctgaa
    20041 aaagaaaacc gcagtcttaa cgatgctgag ggcgcaaaat ttgacgaatt acgcgctaaa
    20101 gctgaatccc tcgataaaga catttcccgc cttgaagcca ttgcagacga agagcgcagc
    20161 aagccaggta aaagcagcca gaccactgac cccgcagaac tacgcaacta cattctgacc
    20221 ggtgaaaccc gcgcattaag tacaggcgtt cccgctgatg gtggttatac cgttatccct
    20281 gaactgaaca ccgaaatcat gcgaatgctg gcggatgaat ccactatgcg ccgcatctgt
    20341 accgtgaaga aaatcagcag caacgagttt aagcagcttg tttccgctgg cggtgcgacc
    20401 gttaaccacg gtgaagaggg taaggcacgc gaacagacca gcaccccgca gattaacgag
    20461 gtgagcatta agctgtatcc ggtctatgcg tacccgcgca ccacacagga aatcgtggat
    20521 ttttccgatg tggacatcct ttcatggctg acgggtgaga ttggcgacac cttcacggaa
    20581 accgaagaaa gcgatctggt tgtgggcgac ggtgacaaaa aagcaaaagg ttttttatcc
    20641 gtaccccgtg cagagaagaa cgacaaagag cgtgattttg gtacgttgca ggtaattaaa
    20701 ccttccgaat ctctggcgtg gacatctgcg gacccgctga tcgacctgaa atttgcatta
    20761 cgtaaaaaat accgcaaaaa cgcggtctgg gtggttaact ccacgacggc ggcaaaactt
    20821 cagaaggtga agaacgcgaa cggtgattat atctggcgcg accgtttaca ggcgggtgat
    20881 cctgatacgt tgctgggcct tccggtcgaa tatctggagt ttatgcctga taacgttatt
    20941 gccctgggtg acttcaaacg cggttactac attgttgatc acgaaacagg tgttcgcacc
    21001 agaccggaca acctgacaga accaggattc atcaaaattt tcacgcaaaa atatttaggc
    21061 ggtggcgtgg tggattcgaa cgcgatcaag attctggaac tgccggaaga cgacgattaa
    21121 cagcatacag aaggggctaa aaagcccctt tagtgtttta tgggtgaaaa aattatgaag
    21181 agtatggaaa tccggtcatc ggaaatcacc accagcggag ccggtacgct gacgggctac
    21241 gttgttcgct gggataagct ttcagaactg ttatggggtg agttttacga aaaattccag
    21301 cggggggcgt ttactgaatg gcttgcggcg ggtaatgacg ttcgcggcct gtatgagcat
    21361 gaccacagca tgttactggg gcgcacccgt tccggcacgc tgaaactgga agaggacgaa
    21421 acagggttac gctttgaact gaccccaccg gataccagta cagggcgcga cgttatcgaa
    21481 ctggttaagc gtggggacat ctcggggatg agctttagct ttcgctcccg taaggatgta
    21541 tgggatacca caacagatcc atgcgtgcgc accgtgctgg tggcggaact gtacgaaatt
    21601 accgttacat cggtaccggc ttaccccgat tccggcgtgg agctggcccg ccgttccctg
    21661 tatgagcagc accccgaaaa aatgccgcgt gaggataatc gccgctggtg ggcggattta
    21721 gcgggggtgt gatatgtggc ctttcagacg aaaaaaagag cagcgcagca tgacgcttga
    21781 tgaatttatg gcgctggccg gcacatcgaa cacgggggcg ggtgagtacg tatcatctgg
    21841 gacagcggaa tcactctccg ccgtcatgaa cgccgtcacg gtcatctctg aggcggtggc
    21901 taccatgccg tgttacctgt acctggtacg caatgagaag gggaaggagg cccgcgaatg
    21961 gcttgattct catccggtcg atcacatcct caacgagcgc ccgaacgcgt ggcagacacc
    22021 ctaccagttt aagcgaatga tgatccgcca ctgcctgtta agtggtaatg cttatgcggt
    22081 tattcagtgg gggcgtgatg gttttccggc ggctttacat ccttacccgc cgcagtcggt
    22141 gaacgtggag cagacaggtg atcataactg gcgctactgc atcactgacg cctacaccgg
    22201 aaacacccgc aactatttac cgtgggaggt tctccacctt cgttactcca cggatgacgg
    22261 ttttatgggg cgctcacctg taaccatctg ccgcgaatcg ctggggcttg ggctggccca
    22321 acaacgccac ggcgcgagcg tgatgcgtga tggcatgatg gcggcagggg ttatcacgtc
    22381 aggcgaatgg ctggacggcg tgaagggcaa acaggcatta gccgcactgg aacgctacaa
    22441 aggggccaga aacgccggaa aaacgcccat ccttgaaggg gggatgagct atcagcagct
    22501 gggcatgagt aatcaggatg ccgaatggct ggcctcccgt cgcttcacca ttgaagacat
    22561 cgcccgaatg ttcaacgtct cgccgatttt tttgcaggaa tacagcaaca gcacctacag
    22621 caacttcagc gaggcaagcc gcgcatttct caccatgacg atgcgcccgt ggctggcgaa
    22681 ctttgagcag cagataaaaa atgccttgct ggtggcctcg cctgtacctg gtatccggta
    22741 tcaggtggag tttgacagcg cggacctgtt acgggccaca cctggcgaac gctttgccac
    22801 ctatgagcgc ggcataaaat ccggcgttat gtgtccgaac gaagcccgcg aacgtgaagg
    22861 gctgtcaccg cgtgatggtg gtgatgagtt cagccaggca tggaaacagg aagtaaaaat
    22921 cagcgaggga gaaaaaccgg aatgaacata gggcgactgc gtgacaggat aacgattcag
    22981 accctgaaac agaccaggga tataaccggc gaaatactcg aaacgtggga ggacggtcac
    23041 acactctggg caagcgtgaa catggtcagc agcaaggagg ccatttcatc gggcgcagag
    23101 ctggcgattg gtaccgtaag gatctggata cggtaccgga aggacatcaa cgccaccagc
    23161 cggataaagg tcagtacggg gccgctggcg gggcgtgtac tgaatatcat cgggcagccg
    23221 ctgccggatg ccgccaggac acgccttgaa attctttgtc gtgagggcgc ggaaaaatga
    23281 cagaagaact tatcaccctg gaagaagtga aactccattg ccgcatcgat ggcgacgatg
    23341 aagaccagtt aatcagcgga tacattgccg catcgcttga ggcgtgccag atacacatag
    23401 gcaggcgctt tgatgacggg ctggcgttca cgccagccat aaagattggc tgcatgatgt
    23461 ttatcgctca cctgtatgaa aatcgccaga ttgtcgcgga taacgcaaaa acacgcgtac
    23521 ccatgacgat aggcgcactc tggacggctt accgtgatgt gggggtgtac tgatgccgtg
    23581 gcaaccatta agacgatgca cagaaccagg ctgtaacagg cgcgttaaat ccggcaagtg
    23641 tgaggagcac aggcgggcgg catggcgtgc agaggatgcc agacgaggac accgccgcgc
    23701 gcgtggatat tccagacagt gggacaaata ccgcgccatg tatctgagta aaaacccgtt
    23761 atgcgtgcga tgccttgaga aggggatata tacgcccgcc gtggtggtgg atcacattat
    23821 cccgattaat ggcggtgatg atgttctctt ctggcccgaa tggaaccatc aaccattgtg
    23881 ccaggcgtgt cataaccaga aaacgaaatg gcttgatccg gcaacaaaaa gcaagcgtgc
    23941 cgcaggtgga tttcgtgaag aggaagaacg ggctgctaac cgcaataact ggatgtatga
    24001 cgctgatgaa tgagcgggaa caaaaccgcc ttatccgtgg acttataagg cagcgtgacg
    24061 catggaagac acaggaaaca gggcataaag ataaagcgtc aggacgcgca gaacgcatca
    24121 cagcgaagcg attaaccgac cgtgaccgcg aggttatgga atgtttccgc aatcgctgat
    24181 gaggctgtat gacggggtgg ggggcgtttt caggacaaac ccgaccccgc cgggcaccga
    24241 acgcctcccc aaatttttat gcacgggaat tttttgaaaa ataatctgac gaaaaataag
    24301 catggcaaga ccaccgaaag cccccgccta cctggatgat atcgccgtga agcagtggcg
    24361 ggaaaaatcg cggcagcttg cggaacgggg agacctgacc cccgccgact ggagcaatct
    24421 ggaactgtat tgcgtcaact actccattta ccggaaagcc gttgcagacc ttgcggcgcg
    24481 cgggttcagc attgttaaca gtcagggcgg cgagagcaga aaccccgcat taagcgcaaa
    24541 atccgacgct gaaagagtga tgataaaaat ggcctccttg ctcggttttg acccgataag
    24601 ccgccgtaaa aatccaccgg aaacagaaga agaggacgag cttgaccgcc tggaataagt
    24661 acgcagaaga cgtaaaaacg ggcaaaattc cggcctgtaa acggttaaaa caggctgtta
    24721 aacggtactt ttcggacctt aaaagccccc tttacatgtt cgatcgtgag gttgtggagc
    24781 ggtttattgc cttttccagg gtgtgcccgc acgtaaaagg accgatgcgt ggcagaccca
    24841 ttgagctgga gccgtggcag cagttcgcct ttgcgtgcat cctcggcttt aaggttaagg
    24901 ccaccggacg gcgcaaatac acgagcgcct ttatcgaagt gccgcgcaaa aatgcgaaat
    24961 ccacgaccgc cgcgattctg gctaactggt ttctgattat ggagaacggt cagcaggata
    25021 tctacaccgc agcggtgagc cgtgaccagg cgcggatcgt gtttgatgat gcgcgtcaga
    25081 tgtgcctttt atcccgaccg ttacgcaggc gggtgaatat tcaggcgcat aaggtgatac
    25141 acccgaaaag caacagcctg ttaaagccgc tggcagcaaa agcggcaacc atcgagggga
    25201 caaaccccag tctttccatc gtggacgaat accacctaca ccccgataac ggcgtttatt
    25261 ccgcgcttga gctggggatg ggcgcacgtc ccgaagggct gttatttgcc attaccacat
    25321 cgggcagtaa cgtcgtttca gcctgtaaac agcactatga ttattgctgc cagatcctgg
    25381 acggcgaaga ggtcaacgat tcaatttttg tactgattta cgaactggac gacaaaaacg
    25441 aggttgatga tccggcgatg tggataaagg cgaaccctaa tattgatgtt tccgtggatc
    25501 gtgaaaaact ggcctcaacc atccagaaag cgcggggtat tccgtcgcag tgggtggaga
    25561 tgatgacaaa gcgatttaat atctggtgtc aggggactac cccgtggatg ggtaatggtg
    25621 catgggctga gtgtgccgga acgttcacgg aggaagattt acacggtcag gagtgttacg
    25681 cggggctgga cttatcatca accagcgata tttccagcgt atgctatgcc tttccggtcg
    25741 gtaaaaccat tatgctgatt tcccgtcatt atctgccgga gttccagcta cagaaccccg
    25801 ccaataaaaa ccgcgctgtc tatcgccagt gggctaaagc gggctggata cgcacaacgc
    25861 caggtgactg cattgattac gaccggatca gagatgacat catgcaggat gcggagaaat
    25921 ttaatatcag gctggtgggc tttgatacgt ggaacgccac gcatctcagg acgcaattac
    25981 agggggctgg ttttgaggtg gagccgttcc cgcaaaccta cctcagattc agtccggcgg
    26041 cgaaatcgtt cgaagttttt gttaaccgca gggtgattgt gcatcgtggc gatccggtgt
    26101 tgtcctggtc gatgagtaac gtcgtgatgc agagtgacgc gaacgccaat atcaagccga
    26161 acaagaaaaa atcaccgaac aagatagacc cgagcgtggc ggcgctgatg gcgtttggta
    26221 cattccaggc aga
//
LOCUS       DADMGC010000047        26136 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.047, whole genome shotgun sequence.
ACCESSION   DADMGC010000047 DADMGC010000000
VERSION     DADMGC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.047"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            401..2794
                     /gene="malP"
                     /locus_tag="JNZ73_001936"
     CDS             401..2794
                     /gene="malP"
                     /locus_tag="JNZ73_001936"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HAY8732233.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            2804..4888
                     /gene="malQ"
                     /locus_tag="JNZ73_001937"
     CDS             2804..4888
                     /gene="malQ"
                     /locus_tag="JNZ73_001937"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HAY8732234.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTTTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(4933..6237)
                     /gene="gntT"
                     /locus_tag="JNZ73_001938"
     CDS             complement(4933..6237)
                     /gene="gntT"
                     /locus_tag="JNZ73_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY8732235.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLANCGGAQRIATTLIAKFGKKHIQWAVVLTGF
                     TVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGPTAIA
                     TIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEEMPSF
                     GVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLN
                     RGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNISPLL
                     MAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDP
                     GFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(6598..7173)
                     /gene="nfuA"
                     /locus_tag="JNZ73_001939"
     CDS             complement(6598..7173)
                     /gene="nfuA"
                     /locus_tag="JNZ73_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HAY8732236.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(7232..7915)
                     /gene="gntX"
                     /locus_tag="JNZ73_001940"
     CDS             complement(7232..7915)
                     /gene="gntX"
                     /locus_tag="JNZ73_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HAY8732237.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            7953..8741
                     /gene="bioH"
                     /locus_tag="JNZ73_001941"
     CDS             7953..8741
                     /gene="bioH"
                     /locus_tag="JNZ73_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HAY8732238.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPYFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            complement(8742..9107)
                     /locus_tag="JNZ73_001942"
     CDS             complement(8742..9107)
                     /locus_tag="JNZ73_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAY8732239.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPCDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(9101..9370)
                     /locus_tag="JNZ73_001943"
     CDS             complement(9101..9370)
                     /locus_tag="JNZ73_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HAY8732240.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            complement(9443..10321)
                     /gene="rpnA"
                     /locus_tag="JNZ73_001944"
     CDS             complement(9443..10321)
                     /gene="rpnA"
                     /locus_tag="JNZ73_001944"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HAY8732241.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(10524..10760)
                     /gene="feoC"
                     /locus_tag="JNZ73_001945"
     CDS             complement(10524..10760)
                     /gene="feoC"
                     /locus_tag="JNZ73_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HAY8732242.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(10760..13081)
                     /gene="feoB"
                     /locus_tag="JNZ73_001946"
     CDS             complement(10760..13081)
                     /gene="feoB"
                     /locus_tag="JNZ73_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HAY8732243.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGMGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(13098..13325)
                     /gene="feoA"
                     /locus_tag="JNZ73_001947"
     CDS             complement(13098..13325)
                     /gene="feoA"
                     /locus_tag="JNZ73_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HAY8732244.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(13763..16084)
                     /locus_tag="JNZ73_001948"
     CDS             complement(13763..16084)
                     /locus_tag="JNZ73_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAY8732245.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNGVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(16181..16693)
                     /gene="greB"
                     /locus_tag="JNZ73_001949"
     CDS             complement(16181..16693)
                     /gene="greB"
                     /locus_tag="JNZ73_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HAY8732246.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANW
                     YVNAIEYVKP"
     gene            16885..17604
                     /gene="ompR"
                     /locus_tag="JNZ73_001950"
     CDS             16885..17604
                     /gene="ompR"
                     /locus_tag="JNZ73_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HAY8732247.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            17601..18953
                     /gene="envZ"
                     /locus_tag="JNZ73_001951"
     CDS             17601..18953
                     /gene="envZ"
                     /locus_tag="JNZ73_001951"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HAY8732248.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(19029..20651)
                     /gene="pckA"
                     /locus_tag="JNZ73_001952"
     CDS             complement(19029..20651)
                     /gene="pckA"
                     /locus_tag="JNZ73_001952"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAY8732249.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            21030..22754
                     /locus_tag="JNZ73_001953"
     CDS             21030..22754
                     /locus_tag="JNZ73_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HAY8732250.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(22889..23767)
                     /gene="hslO"
                     /locus_tag="JNZ73_001954"
     CDS             complement(22889..23767)
                     /gene="hslO"
                     /locus_tag="JNZ73_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAY8732251.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(23792..24193)
                     /gene="hslR"
                     /locus_tag="JNZ73_001955"
     CDS             complement(23792..24193)
                     /gene="hslR"
                     /locus_tag="JNZ73_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HAY8732252.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIKGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(24204..24872)
                     /gene="yrfG"
                     /locus_tag="JNZ73_001956"
     CDS             complement(24204..24872)
                     /gene="yrfG"
                     /locus_tag="JNZ73_001956"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HAY8732253.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(24937..>26136)
                     /locus_tag="JNZ73_001957"
     CDS             complement(24937..>26136)
                     /locus_tag="JNZ73_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator family protein"
                     /protein_id="HAY8732254.1"
                     /translation="DRHVVRQGRYLSLHDEVKNFPLQHWLRSTIIAAGSLLVLFMLLF
                     WIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRVGDTLRISGTGMCNIRTSGTWSA
                     KTNSPFLPFDCSQIIWNDARSLPLPESELVNKATALTEAVNRQLHPKPEDESRVSASL
                     RSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLKNALVNLGNSKDWDALVKRANAG
                     KLDGVNVLLRPVSAESLDNLVATSTAPFITHETARAAQSLNSPAPGGFLIVSDEGSDF
                     VDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNAEGIVTKIFTDANGTQHIGLHPI
                     PDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQRHRTRMMEIQAYYESCLNPQLIT
                     PSESLIE"
BASE COUNT         6147 a         6618 c         7047 g         6324 t
ORIGIN      
        1 atcagattca tttaagcaag ggtttcttct tcattcctga tgaaagtgcc atctaaaaag
       61 atgatcttaa taaatctatt aagaatgaga tggagcacac tggatatttt acttgtgaaa
      121 ctgtttcact cctttactta atttatagag ttaccttccg ctttttgaaa atacgcaacg
      181 gccatttttt gcacttagat acagattttc tgcgctgtat ggcattgatt tgctgctaat
      241 cctgtggttt gcactagctt taagtggtcg agatcacatt tccttgctca tccccgcaac
      301 tcctccctgc ctaatccccc gcaggatgag gaaggtcaac atcgagcctg gcaaactagc
      361 gataacgttg tgttgaaaat ctaagaaaag tggaactcct atgtcacaac ctatttttaa
      421 cgataagcaa tttcaggaag cgctttcacg tcagtggcag cgttatggct taaattctgc
      481 ggctgaaatg actcctcgcc agtggtggct ggcagtgagt gaagcactgg ccgaaatgct
      541 gcgtgctcag ccattcgcca aaccggtggc gaatcagcga catgttaact acatctcaat
      601 ggagtttttg attggtcgtc tgacgggcaa caacctgttg aatctcggct ggtatcagga
      661 tgtacaggat tcgttgaagg cttatgacat caacctgact gacctgctgg aagaagagat
      721 cgacccggcg ctgggtaacg gtggtctggg acgtctggcg gcgtgcttcc tcgactcaat
      781 ggcaactgtc ggtcagtctg cgacgggtta cggtctgaac tatcaatatg gtttgttccg
      841 ccagtctttt gtcgatggca aacaggttga agcgccggat gactggcatc gcagtaacta
      901 cccgtggttc cgccacaacg aagcactgga tgtgcaggta gggattggcg gtaaagtgat
      961 gaaagacgga cgctgggagc cggagtttac cattaccggt caagcgtggg atctccccgt
     1021 tgtcggctat cgtaatggcg tggcgcagcc gctgcgtctg tggcaggcga cgcatgcgca
     1081 tccgtttgat ctaactaaat ttaacgacgg tgatttcctg cgcgccgaac agcaaggcat
     1141 caatgcggaa aaactgacca aagttctcta tccaaacgac aaccatactg ccggtaaaaa
     1201 gctgcgcctg atgcagcaat acttccagtg tgcctgttcg gtagcggata ttttgcgtcg
     1261 ccatcatctg gcggggcgta aactgcacga actggcggat tacgaagtta ttcagctgaa
     1321 cgatacccat ccaactatcg cgattccaga actgctgcgc gtgctgaccg atgagcacca
     1381 gatgagctgg gatgacgcct gggccatcac cagcaaaact ttcgcttaca ccaaccatac
     1441 cctgatgcca gaagcgctgg aacgctggga tgtgaaactg gtgaaaggct tactgccgcg
     1501 ccatatgcag attattaacg aaattaatac tcgctttaaa acgctggtgg agaaaacctg
     1561 gccgggcgat gaaaaagtgt gggccaaact ggcggtggtg cacgataaac aagtgcatat
     1621 ggcgaacctg tgtgtggttg gcggtttcgc ggtgaacggt gttgcggcgc tgcactcgga
     1681 tctggtggtg aaagatctgt tcccggaata tcaccagcta tggccgaaca aattccataa
     1741 cgtcaccaac ggtattaccc cacgtcgctg gatcaaacag tgcaaccctg cactggcggc
     1801 tctgttggat aaatcactgc aaaaagagtg ggctaacgat ctcgatcagc tgatcaatct
     1861 ggaaaaattc gctgatgatg cgaaattccg tcagcaatat cgcgagatca agcaggcgaa
     1921 taaagtccgt ctggcggagt ttgtgaaagt tcgtaccggt attgagatca atccacaggc
     1981 gattttcgat attcagatca aacgtctgca tgagtacaaa cgccagcacc tgaatctgct
     2041 gcatattctg gcgttgtaca aagaaattcg tgaaaacctg caggctgatc gcgtaccgcg
     2101 cgtcttcctc ttcggcgcga aagcggcacc gggctactac ctggcgaaga atattatctt
     2161 tgcgatcaac aaagtggctg acgtgatcaa caacgatcca ctggttggcg ataagttgaa
     2221 ggtggtgttc ctgccggatt attgcgtttc ggcggcggaa aaactgatcc cggcggcgga
     2281 tatctccgaa caaatttcga ctgcaggtaa agaagcttcc ggtaccggca atatgaaact
     2341 ggcgctcaat ggtgcgctta ctgtcggtac gctggatggt gcgaacgttg aaatcgccga
     2401 gaaagtcggt gaagaaaata tctttatttt tggtcatacc gtggaacaag tgaaggctat
     2461 tctggccaaa ggctacgacc cggtgaaatg gcggaagaaa gataaggtgc tggacgcagt
     2521 attgaaagag ctggaaagcg gtaaatacag cgacggcgat aagcatgcct tcgaccagat
     2581 gctgcacagt atcggcaaac agggcggcga tccgtatctg gtgatggcgg atttcgcagc
     2641 ctatgttgag gcacaaaagc aggtggatgt gctgtaccgc gaccaggagg cctggactcg
     2701 cgcggcgatc ctcaataccg cccgctgcgg tatgtttagc tcggatcgct ctattcgcga
     2761 ttatcaggct cgtatctggc aggcaaaacg ctaaggaagc tcgatggaaa gcaaacgtct
     2821 ggataatgcc gcgctggcgg cggggattag ccccaattac atcaatgccc acggtaaacc
     2881 gcagtcgatt agcgccgaaa ccaaacggcg tttgcttgac gcgatgcatc aacgtaccgc
     2941 cacgaaagtg gcggtaacgc cagtcccgaa tgtcatggtt tataccagcg gcaaaaaaat
     3001 gccgatggtg gtggagggca gcggcgaata tagctggcag ctgaccaccg aagaaggaac
     3061 gcagtacaaa ggccatgtaa cggggggcaa agcgttcaat ctaccgacga agctgccgga
     3121 aggttatcac acgctaacac tcacccagga cgaccagcgc gcgcattgcc gggtgattgt
     3181 cgccccgaaa cgttgttacg aaccgcaggc tttgctgaat aaacaaaagc tgtggggtgc
     3241 ctgcgttcag ctttatacgc tgcgatcgga aaaaaactgg ggtattgggg attttggcga
     3301 tctcaaagcg atgctggtgg atgtggcaaa acgtggcggg tcgttcattg gcctgaaccc
     3361 gattcatgcg ctctatccgg caaatccgga gagcgccagc ccatacagcc cgtcttctcg
     3421 ccgttggctg aatgtgattt atatcgacgt taacgccgtt gaagatttcc atcttagcga
     3481 agaggctcag gcctggtggc agttgccgac cacgcaacag acgctgcaac aggcgcgcga
     3541 tgccgactgg gtcgattact ccacggttac cgccctaaaa atgacagcat tacgaatggc
     3601 gtggaaaggt ttcgcgcaac gtgatgatga gcagatggcc gcgtttcgcc agtttgttgc
     3661 agagcagggc gacagcctgt tctggcaggc agcctttgat gcgctacatg cccagcaagt
     3721 gaaagaggac gaaatgcgct ggggctggcc tgcatggcca gagatgtata agaacgtgga
     3781 ttcaccagaa gtgcgtcagt tctgcgaaga acatcgtgat gacgtcgatt tttatctctg
     3841 gttgcagtgg ctggcttaca gccagtttgc cgcctgctgg gagataagcc agggctatga
     3901 aatgccgatt ggcttgtatc gtgatctggc ggttggcgta gcggaaggtg gggcggaaac
     3961 ctggtgtgac cgtgaactat attgcctgaa agcatcggtt ggcgcgccgc cggatatcct
     4021 cggcccgttg gggcagaact ggggattacc gccaatggac ccgcatatca tcaccgcgcg
     4081 tgcctatgaa ccgtttatcg agctgttgcg tgccaatatg caaaactgcg gcgcattacg
     4141 aattgaccat gtgatgtcga tgctgcgttt gtggtggata ccgtatggcg agacggcaga
     4201 tcagggcgcg tatgttcact atccggtgga tgatctgctc tcgattctgg cactcgaaag
     4261 taaacgtcat cgctgtatgg tgattggtga agatctcggt accgtaccgg tagagattgt
     4321 cggtaagctg cgcagcagcg gtgtgtactc ttacaaagtg ctctatttcg aaaacgacca
     4381 cgagaagacg ttccgtgcac cgaaagcgta tccggagcag tcgatggcgg ttgcggcgac
     4441 acatgacctg ccaacgctgc gtggttactg ggagagcggg gatctaacgc tgggcaaaac
     4501 cctggggctg tatccggatg aagtggtact gcgcggtctg tatcaggatc gcgaactggc
     4561 gaagcaaggg ctgctggatg cactgcataa atatggttgt ctgccaaaac gtgccgggca
     4621 taaggcatcg ttgatgtcga tgacgacgac gctgaaccgt ggtttgcagc gctacattgc
     4681 cgacagtaac agtgctctgt taggactaca gccggaagac tggctggata tggccgaacc
     4741 ggtgaatatt cctggcacca gttaccagta taaaaactgg cgacgcaagc tttccgcaac
     4801 gcttgagtcg atgtttgccg atgatggcgt gaacaagttg ctgaaggatt tggacagacg
     4861 gcgcagagct gcagcgaaga agaagtagag tgcggttgat gtcggatgcg atgcatccgg
     4921 cagtcagtgt tatcaaatca ccatattcag cagcagacag cctaccagac cgcacaccga
     4981 gataatggtt tccagcatcg accaggattt gatggtctcg ccgatagtca ggttaaagta
     5041 ctctttgaac agccagaagc ccggatcgtt cacatgagag aaaatcacac taccggaacc
     5101 caccgcaata accatcagct ctgggctaac acccgtcgtt gcaatcagcg gtgccgcgat
     5161 accacccgca gtgattgccg caacggttgc ggaacccagc gcgatacgca gtacggcagc
     5221 aatcgaccag gccatcagca gcggagaaat gttggtttcg tgcatcatgg aagcaatgta
     5281 tttgtccacg ccgctgtcta ccagcacctg cttgaacgca ccgccaccac cgatgatcaa
     5341 cagcatcatc gcaatgattt tgatggaaga aaccagcgtg tcgttaatct gatccattga
     5401 acgaccacgg ttcagaccaa aggtgaacat cgcaatcagc acggcaatca gcgttgccat
     5461 taccgggtca ccgaggaact ccgctaccgg caggaaagcg tgacctttcg gcaggatcat
     5521 ttcggcaatc gcacgcatcg ccatcagtac aaccggcacc agagaggtcc agacgctgac
     5581 gccaaagctc ggcatctctt cttcgctgaa ggttttcgcg ctgtagagac cttccgggat
     5641 tggcttatcg atacctttca acacgcgagc gtaaaccgga ccggcgagaa tcacggtcgg
     5701 gattgccaga atagtaccgt acagcagggt tttacccata tcggcattga aaatggtggc
     5761 aatcgcagtc gggcccggat gcggtggcag gaagccgtgg gtcacagaca gtgcagccgc
     5821 cattggtaca ccaacataca gcaatggaat attcgcggaa gccgcgatgg taaacaccag
     5881 cggcagcatc agcacgaagc ccacttcata gaacagggca aaaccaacgg taaaaccggt
     5941 taacaccacc gcccactgga tgtgcttttt accaaatttg gcaatcagcg tggtggcgat
     6001 acgttgtgcg ccaccgcagt ttgccagcat tgcgccaaaa cccatgatca gggcaaggct
     6061 accgagcgtc ccgccgacac cggctttgat ggagccaata actttatcca gcggcattcc
     6121 ttgcattaat ccaacagcaa gcgccacgag gacgagagcg atgaagccgt tcattttgaa
     6181 gcggatcatc aggagcaaca acaagattac accgatagca acaatgacta atggcatgat
     6241 ttacctggcc tttcatttgt tatgggtaat gtcaattttc tgacgacaaa ctctaattat
     6301 cccaatcggg aacagagata ttgcggcacc acgactgata cccactaaaa ctaattattg
     6361 tagtcagatg tcaggagtat gtttggtacc catgtgaatg atacgggtaa catctggcgt
     6421 ttgagaatca ccagagcggg gtaaatttaa attatgagag gttggtcata ttatcgcggg
     6481 gaaacgaagg gagggatttg ccaaagcaat gctgtgccaa cgtctggcac atgttcaacg
     6541 taggcccgaa atgacgcttt agcgtcgcat cgggcaatct acaaaagagg ggataactta
     6601 gtagtaggag tgttcgccgc gctggtgttc ggtgagatcg cgcacacctt tcagctccgg
     6661 gaattcgttc agcagctgct tctcgatccc ttctttcagc gtcacatcga ccatggaaca
     6721 accgttacag ccgccgccaa attgcagaat ggcgtaaccg tcttcggtga tttccatcag
     6781 cgaaacgcga ccaccgtgac cagcaagctg tgggttgatc tgcgactgca gcatatactc
     6841 cacgcgctcc atcagcggtg catcgtctgc cactttacgc attttggcgt tcggggcttt
     6901 cagcgttaac tgagaaccca actggtcggt aacaaagtcg atctctgcat cttccaggta
     6961 tggtgcgctt aactcatcaa catacgcggt cagcaggtca aatttcaggg cagtgtcggt
     7021 ggcttccacg gcatccggcg gacaataaga aacaccacat tcagcgttag gcgtgccagg
     7081 gttaatcaca aatacgcgga tttgtgtccc ttcttcctga tttgccagca gtttggcaaa
     7141 gtgcgcttgt gcagcatcgg aaatacggat catagtaatg gcctaatagt tgactatttt
     7201 agttggttat aatacgccca tcatcgaggc tctacaaggt tcgacaaagg caccagacct
     7261 ggacagtcgc cgcaccattg cgtaaaagca actgcgcaat ctctgcgacg gtacttccgg
     7321 tggtaacgac atcatccaca atcaccatat ggcgaccttg cacgggcaat tcaagacgaa
     7381 aggcattttt caggttgcgc ttgcgcagcc gggcactgag aaaatgctgg gtcgcagtgg
     7441 cccgtgtacg tgtgacggct tcgctatccc attggcagtg caaccagcgt gataacggct
     7501 gacacagcaa atcgctctga ttaaatcccc gacgccagtg acgccgctgc cataacggaa
     7561 cgctgatgat gcgatccggc aattgcaacc cggtggtgcg acgagcgtgt aagacttcca
     7621 atagtaacag acgtgacagg gcgctggcga tttcactgcg ccgggaaaat ttaagctggt
     7681 ggataagcgg acttaacggc ggcgcatagt cggcaaccgt gaccagtctt tgccagggcg
     7741 gcggtttttg caggcagcga ccgcagggaa gatgggagtg tgtggcgggt aatccacatt
     7801 gtgggcataa cgttttatct gtgcgggtgg cgcgtgaaca gaccgaacaa atcccccaat
     7861 gacctaacgc cagtggcatt cggcatagcc agcataatcc cggtactgtt agcatatgtt
     7921 catccttgta agtcaaaaga gaacaatagc ggatgaataa catctggtgg cagaccaaag
     7981 gtcaggggaa tgttcatctt gtgctgctgc acggatgggg actgaatgcc gaagtgtggc
     8041 gttgcattga cgaggaactt agctcgcatt ttacgctgca ccttgttgac ctgcccggct
     8101 tcgggcgtag ccggggattt ggtgcgctgt cacttgctga tatggccgaa gccgtgctgc
     8161 aacaggcacc tgataaagcc atttggttag gctggagtct gggcgggctg gtggcaagcc
     8221 agattgcgtt aacccatccc gagcgtgttc aggcgctggt caccgtggcg tcgtcacctt
     8281 attttagtgc tcgtgacgag tggccgggga taaaaccgga cgtgctggcg ggatttcagc
     8341 agcaactcag tgatgatttt cagcgtacag tggagcggtt cctggcgtta caaaccatgg
     8401 ggactgaaac ggcgcgccag gatgcgcggg cgttgaagaa aaccgttctg gcgttaccga
     8461 tgccggaggt tgacgtgctt aatggcgggc tggaaatcct gaaaacggtc gatctccgtc
     8521 agccgctgca aaacgtgtcc atgccgtttt tgcgattgta tggctatctc gacggtctgg
     8581 tgccgcgcaa agtggtgccg atgctggata aactttggcc tcacagcgaa tcatatatct
     8641 tcgccaaagc ggcccatgcg ccgtttattt cgcatccggt cgagttttgt cacctgctgg
     8701 tggcgttgaa gcagagggtg ttggtggtaa gtgagagcta attattgtgg gtcgattaaa
     8761 ttcaccagtt tactgataac ttcactaatc aatttttcat cggcaacggt atgaaattta
     8821 gcgccacggg caataagatc gacggctttt agctggtgac aaagtacaac gccatgcagg
     8881 ttaccggttt gcgtatcaca ggggaggacg ttcaccgtca cccctgtgga acgtgctgca
     8941 tttgcccctg ttgaaatcgg gcagcacata gcaactttta aaacattgtt gagttttttg
     9001 tccgttacca caatgcaata atgtggcccc tgaagttcat gcccggcaac cggatcaggg
     9061 ttaaaatacc agatctcacc acgatgtggc gtccgtgcct ttaccatgct tcattacctt
     9121 ttttcccttc agcccatcct tctgtggact gatttagctc cgtaatttcg ctttcatcga
     9181 tttggctcaa caactgcgcg acggtaagac gcccacgcgg tttgtgctct gtggcgcgca
     9241 aattaacgct atcgcctttt ttctccacgg taagctctgt gcccggtgac cagccaagat
     9301 cgatggcaag atctcgtggg atagtaacga tcatggctcc tccttgttgc cgtaatttca
     9361 ctgtctgcat atcagttccc taagtgctac tttgtgttac atgcaagata gcacaaagta
     9421 gcacttaagc agggtagagt atctactgac tcgccgcagc caactcttct tctgacagcc
     9481 cggtaaatcg catgatgtct gcaagaggga ctccggattc aagcattatt ttggctatat
     9541 gcagggcttt ggattgttcc ccctcctgcc gcaatctttc cgcaatagtc attaaactct
     9601 ccttgtgttt cggtgaacgt tcggcaacgc cgtcgataaa atcgttaaaa cgtacagcgt
     9661 cgccagtttg cagtatgtaa ttaaacagcc ctttgatttg tctgtcatta gcgtatccac
     9721 tacttaataa gcaggccatt tgctctacca gccccatcag atcgcgttga cgaatatgtt
     9781 tttgaattaa ctccagcagc gccatacgtc ggtgctgcat gatttcatca tcaggcatga
     9841 cggtgacatc aatcagcgga aatgcggagg cataaagctg ccttgccagt ttgggatcgg
     9901 cgaaacaatc cagccaacac agcgaatagg gataggggct ttcaataccg tggtaaaaca
     9961 acaatggcac caccatcggc aacgttttgt atccagcatc aaggtgattt tgcattgcgg
    10021 caatagcgta acgcatcatg cgaaatgcga tcagtttgtt tgaggtgctt tgatgttcaa
    10081 tcagacaata gatgtatcct ggtccttgtt ccgttttcac cgaccacagc acatcggaat
    10141 agctttcacg cagatcgtca tcaataaagc tgctcgactc cagcttgagg gttttcatat
    10201 cacagagcgc gtgaatgggt gccggtaaat gaaacgcaag aaaatcgcgt gccgtctccg
    10261 gttggcgtaa aaagagtttg aacagcgcat catgtggggt ggaactctgc tttttactca
    10321 tggcgttcct tcacctgttg tttgaatgcc gcgactctac gcgccactcc acatctgtgc
    10381 attagcgagt tttcatccct gcgagacgct ttcagaggaa cttacgttgc gctgcaagaa
    10441 ttgcattttt atggaaagcc gcacgcaaaa aagcccggat gcaaacgcat accgggcttt
    10501 tcaaaacagg cgatggagta tggttaacgc agcgcccacc actcgcgcag acaggctttg
    10561 ccttccgggc agcttttaca actgccagag aggcagccgt caggttcttc ctgaatccgc
    10621 acggctttgc ccatgctttc cagctgttgc agcatggcgt taatcattgg ctgcggagtg
    10681 ttcaatgtct ggcttatctg ggtcgcttcc atacggcccc gtaacgccag caaatcacgt
    10741 acctgaataa gtgaagccat taatggcaat caccggtggt gctggctgcg cagcaactgc
    10801 ttaccgactt gcgggttgcc agcagttcga tatccacccg gctacgcgcg cggcgcagca
    10861 gaccgataac tacgatgtta aacaggatta ccgccagaat gcacaccagg ctgtaagttg
    10921 ggtgctgact gtagctggcg acctgataga acaatgttgc cagtgagtaa gcgatattca
    10981 gcccccacag gatggagaag cccatccagc cacggcttga ttcacgggcg atggctccca
    11041 tcaccgagat acatggtaca tacagcagga cgaaaatcag gtagctgtat gcggcagctg
    11101 cgctaccgaa tttctgatcc atcacgccca tcgccccggt acccatttca ccgtcgcctt
    11161 tgctagcttc aatggggttc atcagtacgc taaggctgaa ggtgtctttc aggctctgcc
    11221 aggtttcatc taccgcactg aacagctctt caccgaggtt gaattccgcc ggattgaact
    11281 cttcgtcctg aatattttct gcggtgtaga gggtgttgag cgtacccacc accacttctt
    11341 ttgccattgc gcccgtaaac aggccaaccg ttgcctgcca gttatcttca tgtacgccaa
    11401 tcggcttgaa gacaggggtt atcacccggc tgacggaagc cagcgccgag tcgttgatgt
    11461 tatcgacgat tttcccgctc aacgagaagc tgttgaaagc gctcaggaaa atgctgacga
    11521 tgataatcac tttaccagca cgcagaacga agcccttcag acgctgccag gtctggataa
    11581 tcaggctttt aacgtgtggt acatggtata ccggcagctc catgacaaac ggcgttgctt
    11641 caccgcgcat gatggtgtac ttgagcatca ggccagtcag caccgccatc acgataccga
    11701 gcatatacag cgagaaaacc gccagggcac cgttctgccc gaagaaggca gccgcgaata
    11761 ctgcgaagat agccagacgc gcgccgcagg acataaacgg tgccatcatg atggtcatca
    11821 gacgttcacg cggtgcatca agcgtacgtg cgcccattac cgacggtacg ttacaaccga
    11881 aaccgacgat cagcggcaca aaggatttcc ccggcaagcc cagcgcctgc atcaggcggt
    11941 ccatcacaaa cgctgcacgc gccatatagc cggagtcctc aaggaaggag aggaacaggt
    12001 acatcatgcc gatctgcggc accagtggca gcacggtgtt aataccgcca cccatgccct
    12061 gggcgaggaa gatagtcagc cagtccggga agtggagcgt gtagccaatc cattgaatac
    12121 catgcacaaa tagcgccacg gagccgacgt caaacagcgg ctgtaacgcc ccgccgatgt
    12181 tgatagccag caggaacatc aggtacatca caaagaggaa aatcggcaga ccgaggaaac
    12241 ggttgagcac gattttatct accgcagtgg tgaaacggct gggttctgcc gtcagggtgt
    12301 tgcttaccac atcacagatg gcagcaatgc actggtaacg cgcatcggca atgtgcagcg
    12361 ccggatcatc catttcatta cgcagacggg cgagggtggc atccagatgc tgcgacgctt
    12421 caccggcgta ggcgcggctg tagatatcgc cttccagcat ttgcaggccc agccagcgac
    12481 gttgtttcag cgggatgtcg gaaggcatca cttttgccag tgaatctgct tcgttgagta
    12541 gcggctgtgc gtaatgcacc agttccacat tctcgttagc tttatagcga tcaatcgcca
    12601 gcttgagcgc ttcaataccg cgaccacggg ttgaaaccag cgggatcacc gggcagccaa
    12661 gacgtgccga tagagcatca atttcaatac gaatattttg cttctcggca atgtcgagca
    12721 tgttcagcgc cacgatgcag ggaatgccga gttccagcag ttgcagcgtc aggtacaggt
    12781 tacgctcaag gttagacgca tccaccacgt taatcagcag gtcggcgtcg ccactcaaaa
    12841 tgtagtgaca ggcgatttgc tcatcgagcg aggtctgcga tgagatggtg gtcagagaat
    12901 aggtgccggg caggtccacc agcgtgacct gatgatcggt ggtggagaat tgcccttctt
    12961 tacgttcgac ggtaacgcca gcccagttac ctacacgctg acgtgcgcca gtgagctggt
    13021 taaataaggt tgtcttgcca gaatttggat taccaattaa gccaatggtt aattttttca
    13081 ttgttgttat caccgtatta acaggaaacc gcttccactt ctaataaggc cagatctttt
    13141 ttgcgtaata ccaggctcac acgacgggtt tcgatatgaa tggggtcgcc gagtggagcg
    13201 acgcgcacca catcaaaaga ggagccaggt aacatgccta gagaaagcag tttttggcga
    13261 tatgccgggc tgatttcacg ggaaaagcca gtgattttcc acgcagtatc tggagtgtat
    13321 tgcataggtg cctacttgtt tctcattaac tggataacta cctcatgggg cgtttgaccg
    13381 cgacatgaga ccagatgatt tgccaacaaa ggagagatta aataatcaac aacacaatga
    13441 taatgagaat ggtttctatc agcaatactt aaaatgtgta cgaatgttgg tttgaataat
    13501 aatctgactc aatgttgatg tggctcaagg ttatatcgca gcgaaaatat aattcgaata
    13561 gtgagttacc cggactggct aatgtttaat taaggtttca gttgttaatg aagtgataaa
    13621 tattaacgga gtatattgtg agtttggctg tggaataaaa gtgccggaga tattctccgg
    13681 cacttttatg tcaggcaaag cgttcgttgc cagatgcggc gtgaacgcct tatcgcgcag
    13741 gtttaggggc cagtccgggc gtttaacgtt ttttacccat cgctgccgcc agcgcatcca
    13801 tcatcgcgct attaccggca ggctgcgctt cacgaccgcg tggtttggct gccgggcggt
    13861 tgttttgcgg gcgttcatta ccgccgccgc gacgagagtt ggtttcgcca ggctgctcgt
    13921 ccagacgcat agtcagggcg atacgtttac gctgaagatc cacttccagc actttcacct
    13981 tcacaatgtc gcccgctttc accacggtat gcggatcttc cacaaacttg ttcgacaatg
    14041 aagagatgtg aaccaggccg tcctgatgca cgccaatatc gacaaacgcg ccaaagttgg
    14101 tgacgttggt cactgcgcct tcgaggatca tacccggttg caggtcgttc attgtctcga
    14161 cgccatcggc aaactgagcg gttttaaatt ccggacgcgg atcgcgaccc ggtttttcca
    14221 gctctttgat gatgtcagtt actgtcggca caccgaattt ttcatcagta aagtcagacg
    14281 ctttcaggtt acgcagttcg ctgctgttac ccatcagatc tttcagtgcc tgctgtgttg
    14341 ctgccagaat gcgttccacc accggatagg cttccggatg aacggtagag gcgtccagcg
    14401 ggttatcacc gtggttaatg cgcaggaagc ccgcgcactg ctcgaaggct ttcggcccca
    14461 gacggctgac tttcagcagt tgctgacggt tctggaactg gccgttctca tcgcgccagg
    14521 ccacgatgtt ttgcgccatc atgcgcgtca ggcccgccac gcgggttaat agcggaacag
    14581 aagcggtgtt gagatcgacg ccaacgccgt ttacgcagtc ttctactact gcatccagtt
    14641 tgcgggccag ttgcgtctgg ctgacgtcat gctgatactg acctacgccg atagatttcg
    14701 gatcgatttt caccagctcc gccagcggat cctgcaaacg gcgggcgata gataccgcac
    14761 cacgcagcga aacgtcgaga tccgggaact cctgcgctgc cagctcggaa gctgagtaaa
    14821 ccgacgcgcc agcttcgctg acgatcactt tctgtgcggt cactttcggg aactgcttct
    14881 gcacgtcgag atagaaacgt tcagtttcgc gggaagctgt accgttgccg atcgccacca
    14941 gttcaacgtt atgtttttca cacaaggcag caacggtcat cgctgctttt gcggcttgtc
    15001 cggtgtgcgg gtaaatggta tcggtcgcca ccagtttgcc agtggcatcg accaccgcca
    15061 cttttacccc ggtacgcaga cccggatcga ggcccatcgt tgcacgcagt ccggcagggg
    15121 ccgccatcag cagatcgtgc aggttacggg caaagacgtt gattgcttca tcttccgcgc
    15181 gttcgcgcac ggtacccatc agttcggttt ccagatgcat cagcaccttg atgcgccagg
    15241 tccagctcac tacgcttttg cgccagctat ccgccggggc attgttcagg cgcaggccaa
    15301 ggtgatccat gatgatttgc tcgcaatagc tctctttggg cggctcatcg aactgcggat
    15361 cggcattcag cgaaagctgg agtacgcctt cgttacgccc acggaacatc gccagcgcgc
    15421 ggtgagaagg caccgtggac aacggttcgt gatgatcgaa atagtcgcgg aatttcgccc
    15481 cttcctcttc tttaccgctc accaccgtag aaaccaaatg cgcgttcttc cacagataat
    15541 cacgcacttt cgccagcagc gcggcatctt cggcaaaccg ttccatcagg atatagcgcg
    15601 cgccatccag cgcggctttg gtatctgcca cgcctttatc ggcatcaaca tattgtgcag
    15661 cggcgacttc tggcgtgtgt gacggatcgc tccacagcag gtcagccaac ggctcaagcc
    15721 ctgcttcaat ggcgatttgc ccgcgggtgc ggcgtttagg tttgtagggc aggtagaggt
    15781 cttcgagttc ggttttgctt agggtggcgt tgatggcctt cgccagatca tcggtgagtt
    15841 tgccttgctc ggaaatggat ttgaggatcg cctgacgtct ctcttccagc tcgcgcagat
    15901 agctcagacg cgtttccaga ttacgcagct gcgtgtcatc cagaccgccg gtgatttcct
    15961 tacgataacg tgcgataaac ggcacggtat tcccttcatc aagcaggcga acggcagcgt
    16021 caacctgttc cgggcgcgcc tgaatttcac ccgcaataat gcggcagaac gaatcattca
    16081 tcatggtttg agttcatctt ttcggatcaa aaatcagggg atagttatac ggactggctg
    16141 gcaaaaatgc cagccatcgg caggaggtta agactcttcc ttacggtttc acgtactcga
    16201 tagcattaac ataccagttc gcttccccgg caggggtatt caccaccgcc agatcgccga
    16261 cttctttttt cagcaatgcg cgggccatcg gggaatcgat agagatgtaa tctttacggc
    16321 caaaaatttc atcgtagccg acaatacgga aacggtgagt cacgccatcg tcgttttcaa
    16381 tctccaccca cgcgccaaaa aagactttgc cttcctgctg aggggagtaa tcgacaattt
    16441 tgagattttc caggcattta gtgagatagc gcacgcgacg gtcgatttca cgcagacgct
    16501 ttttattata ctgatagtca gcattttcgc tgcggtcgcc cagacttgcg gcccaggtca
    16561 ccttttttgt gacctccggg cgttcttcac gccagagata attaagctct tgtttgagtt
    16621 tttcataccc ttcccgggta accaggggcg ttttcatctc gttgattccc tttgtctgtt
    16681 tgataatgcg cacattgggt ataacgtgat catatcaaca gaatcaataa tgtttcgccg
    16741 aataaattgt atacttaagc tgctgtttaa tatgctttgt aacaatttag gctgaaattc
    16801 ataccagatt tagctggtga cgaacgtgag cttttttaag aatacacgct tacaaattgt
    16861 tgcgaacctt tgggagtaca aacaatgcaa gagaactaca agattctggt ggtcgatgac
    16921 gacatgcgcc tgcgtgcgct gctggaacgt tatctcaccg aacaaggctt ccaggttcga
    16981 agcgtcgcta atgcagaaca gatggatcgc ctgctgactc gtgaatcttt ccatcttatg
    17041 gtactggatt taatgttacc tggtgaagat ggcttgtcga tttgccgacg tcttcgtagt
    17101 cagagcaacc cgatgccgat cattatggtg acggcgaaag gggaagaagt ggaccgtatc
    17161 gtaggcctgg agattggcgc tgacgactac attccaaaac cgtttaaccc gcgtgaactg
    17221 ctggcccgta ttcgtgcggt gctgcgtcgt caggcgaacg aactgccagg cgcaccgtca
    17281 caggaagagg cggtaattgc tttcggtaag ttcaaactta acctcggtac gcgcgaaatg
    17341 ttccgcgaag atgagccgat gccgctcacc agcggtgagt ttgcggtact gaaggcactg
    17401 gtcagccatc cgcgtgagcc gctctcccgc gataagctga tgaaccttgc ccgtggtcgt
    17461 gaatattccg caatggaacg ctccatcgac gtgcagattt cgcgtctgcg ccgcatggta
    17521 gaagaagatc cagcgcatcc gcgttacatt cagaccgttt ggggcctggg ctacgtcttt
    17581 gtaccggacg gctctaaagc atgaggcgat tgcgcttctc gccacgaagt tcatttgccc
    17641 gtacgttatt gctcatcgtc accttgctgt tcgccagcct ggtgacgact tatctggtgg
    17701 tgctgaactt cgcgattttg ccgagcctcc agcagtttaa taaagtcctc gcgtacgaag
    17761 tgcgtatgtt gatgaccgat aaactgcaac tggaggacgg cacgcagttg gttgtgcctc
    17821 ccgctttccg tcgggagatc taccgtgagc tggggatctc tctctactcc aacgaggctg
    17881 ccgaagaggc aggtctgcgt tgggcgcaac actatgaatt cttaagccat cagatggcac
    17941 agcaactggg cggcccgacg gaagtgcgcg ttgaggtcaa caaaagttcg cctgtcgtct
    18001 ggctgaaaac ctggctgtcg cccaatatct gggtacgcgt gccgctgacc gaaattcatc
    18061 agggcgattt ctctccgctg ttccgctata cgctggcgat tatgctattg gcgataggcg
    18121 gggcgtggct gtttattcgt atccagaacc gaccgttggt cgatctcgaa cacgcagcct
    18181 tgcaggttgg taaagggatt attccgccgc cgctgcgtga atatggcgct tcggaggtgc
    18241 gttccgttac ccgtgccttt aaccatatgg cggctggtgt taagcaactg gcggatgacc
    18301 gcacgctgct gatggcgggg gtaagtcacg acttgcgcac gccgctgacg cgtattcgcc
    18361 tggcgactga gatgatgagc gagcaggatg gctatctggc agaatcgatc aataaagata
    18421 tcgaagagtg caacgccatc attgagcagt ttatcgacta cctgcgcacc gggcaggaga
    18481 tgccgatgga aatggcggat cttaatgcag tgctcggtga ggtgattgct gccgaaagtg
    18541 gctatgagcg ggaaattgaa accgcgcttt accccggcag cattgaagtg aaaatgcacc
    18601 cgctgtcgat caaacgcgcg gtggcgaata tggtggtcaa cgccgcccgt tatggcaatg
    18661 gctggatcaa agtcagcagc ggaacggagc cgaatcgcgc ctggttccag gtggaagatg
    18721 acggtccggg aattgcgccg gaacaacgta agcacctgtt ccagccgttt gtccgcggcg
    18781 acagtgcgcg caccattagc ggcacgggat tagggctggc aattgtgcag cgtatcgtgg
    18841 ataaccataa cgggatgctg gagcttggca ccagcgagcg gggcgggctt tccattcgcg
    18901 cctggctgcc agtgccggta acgcgggcgc agggcacgac aaaagaaggg taaataaacg
    18961 ggaggcgaag gtgcctcccg ttttgctttc tataagatac tggatagata ttctccagct
    19021 tcaaatcatt acagtttcgg accagccgct accagcgcgg cacccgcagg ggtgtcggtg
    19081 tatttatcga agttgtcgat aaacagtttc gccagggttt cggctttttc ctgccactgt
    19141 tccggagaag cgtaggtgtt acgcggatcg agaatcttcg tgtctacgcc cggcagttcg
    19201 gttgggatcg ccaggttaaa catcggcaga gtgaaggttt ctgcattatc cagtgaaccg
    19261 ttgaggatgg cgtcgataat ggcgcgggta tctttaatcg agatacgttt gccagtgccg
    19321 ttccagccag tgttaaccag ataagcctgc gcgcccgccg cctgcatacg tttcaccagc
    19381 acttctgcgt actgagtcgg gtgcagcgac aggaacgccg cgccgaagca agcggagaag
    19441 gttggcgtcg gttcggtgat gccacgctca gtaccggcca gtttggcggt gaagccagag
    19501 aggaagtgat actgggtttg atcggcagtc aggcgagaaa ccggcggcaa cacgccgaaa
    19561 gcatcagcag tcaggaagat aaccttagtc gcgtggcccg ctttggaaac cggcttaacg
    19621 atgttatcga tgtgatagat tggataagaa acgcgggtgt tctcggtttt tgaaccatca
    19681 tcaaagtcga tagtgccatc ttcacgcacg gtgacgtttt ccagcagcgc atcacgacgg
    19741 atagcgttgt agatttcagg ttccgcttct ttcgacagct tgatagtttt tgcgtagcag
    19801 ccgccttcga aattaaacac gccgtcatcg tcccagccgt gttcgtcatc gccaatcagg
    19861 cggcgtttcg ggtcggtgga aagggtggtt ttaccggtgc cggaaaggcc gaagaacacc
    19921 gcaacatcgc ctttctcgcc aacgttggcg gagcagtgca tagaagcgat acctttcagc
    19981 ggcagcaggt agttcatcat cgagaacatc cctttcttca tttcgccgcc gtaccaggtg
    20041 ccgccaatca gctgcatgcg ctcggtcagg ttaaacgcca cgaagttttc ggagttcaga
    20101 ccctgttctt tccactgcgg gttagtgcac ttcgcgccgt tcataacgat aaagtctggt
    20161 ttgaaacctg ccagctcttc atcgctcggg cgaataaaca tgtttttgac aaaatgcgcc
    20221 tgccaggcca cttcggtgat gaaacggacg gaaagacgag tatccgggtt cgcaccacag
    20281 aaagcgtcga caacgaacag acgtttgccg gaaagctgcc tggtcaccag gcctttcaga
    20341 tgctgccagg tttccggaga gagaggtttg ttgtcgttct tacctttgcc tttgtctgcc
    20401 caccagaaag tatcgcgagt ggtatcgtca cggacgatat acttatcttt tggtgaacga
    20461 ccggtgaaga tcccggtatc gacggcaacg gcacccagat tagttaacac cccgcgctca
    20521 taacctgtca ggctcggatc gagctcttcc tgatacagca ggtcgtagct tgggttgtaa
    20581 acgatatcat gtacgtcact gataccataa gcctcgagtt cttgcggggt caaaccattg
    20641 ttaacgcgca tttcactgct ccttagccaa tatgtattgc ctgaatagta aagtcttttt
    20701 gggggtgtta accgcgacaa ggctcataga tttacgtatc tggagaaatt catatttgac
    20761 aaaaaactcc gtgattcctg tcacgaaacg gttgctatta tcgcagaaat agcattttag
    20821 gtgtggaaaa tgttctcaaa aggttaaatc ttggtttata tggcgggaat attgtgagtg
    20881 gaatcgcatt cctgaatgaa attgatgcaa attcaaccgg taaagtatcg attcaccacg
    20941 ctaacttttt ttgattattt tctgaaaaaa gcaaaatact actgcccgga tgcgataatt
    21001 aacccacctt ttgattctgg ataaccaaca tggataacgt tgaactttca ccggcaacac
    21061 gctgggggat gattgctacc ggattacttc aggggctggt ttgctacctg cttatcgcct
    21121 ggcttgccgg aaaaaatcac agttggattg tttatggcgt gcctgcgacc gtcgcgtttt
    21181 catccgtttt actcttctcc gtgatctctt ttaaacagaa gcgcctttgg ggatggctgg
    21241 cgctggtgtt tattgccacg acagggatga gcggttggct gaagtggcaa actgatggca
    21301 tgaacccctg gagagctgaa aaggcgattt gggattttgg ctgctatctg ctgttgatgg
    21361 caatgttgtt gctaccgtgg atacaacaaa gccttcgtat ccgcaatggc agtagccgct
    21421 atagctactt ttaccagtca gtatggcata acgtacttat attactggtg atttttctct
    21481 ccaatggcct gacgtggctg gtgcttttac tgtggggtga gttgtttaaa cttgttggca
    21541 tcacgttttt caatacgctc ttttttgcaa ccgactggtt tatgtatctt acatttggtc
    21601 tggttaccgc actggcggtg atcctcgcgc gtacacagtc acgtttaatc gactctattc
    21661 aaaagttgtt cacgctaatc gccacggggt tgctgccgtt agtatcattg ctaaccctga
    21721 tgtttatcat caccctgccg tttacgggcc tgagcgcgat ttctcgccac atctccgccg
    21781 ccgggttgct gttaacgctg gcctttttgc aattgatctt aatggctatt gtccgcgatc
    21841 cgcaaaaagc gtcacttccc tggacggggc cgttgcgttg cctgattaaa accgctttgc
    21901 tggttgctcc gctgtatgtg ttcgtcgcca cctgggggtt atggctgcgg gtcgctcagt
    21961 acggctggac tgtcgaccgc ttgcagggcg cgctggcgga gctggtgtta ctggtgtggt
    22021 cgctggggta ttttgtcagt atcgtctggc gtaatgggca aaatcccctt gttcttcagg
    22081 gcaaagtgaa ccttgcggtt tcgttattgg tgttggtgat actggtgctt cttaattcgc
    22141 cggtgctgga cagtatgcgt attagcgtga acagtcatat ggcgcgttat cagagcggca
    22201 aaaacacgcc agaccaggta actatctaca tgctcgaaca gagcggtcgc tatggacgtg
    22261 cggcgcttga gtcgctgaaa agcgatgccg ggtttatgaa agacccgaaa cgcgcgcggg
    22321 atctgctgat ggcgttagat ggagagcaac atcttcagga acaggtatcg gaaaaagtat
    22381 tagccgaaaa tgtgttaatt gcccctggtt ctgttaaacc ggatgcgaca ttctggtcgg
    22441 ccttaatcca ggatcgctat aacgtgatga cctgtattga aaaagacgcc tgcgtactgg
    22501 tcgagcaaga tctgaatagt gatggtcagg cggagcggat cctgtttgct tttaatgatg
    22561 acagagtcat tgtctatggc tttgactcag acagaaaaga atgggacgcg cttgatatga
    22621 gtttacttcc gaacgaaata acgaaagaaa aattactcac agctgcgaag gatggaaaac
    22681 tggggacgag gccgaaagcg tggcgcgatc tgacggtaga tggtgaaacg ctggaaataa
    22741 acttaagtaa gtgacaacgc ttttatcgga tacgacgctg tatctgacca aaataatttg
    22801 cgatttattt cataggcgct ggtaagcgca gcacatcagg ccattaagtc gtaaaatccg
    22861 gcagagccct ctctgccgga catactcatt aatgaacttg cggatctgcc ggagacgcgt
    22921 tgttgcggat ttcagcaata tccatcgcat tgaacagata gtggttaccg cagtaatcac
    22981 aatgcatgtc aatttcgcca tcttccgcca ggatgctatc aacttcttca tcaggcagcg
    23041 ttttcagcgc atcggcgcaa cgttcacgcg agcaggtgca tttgaactcc acatcctgcg
    23101 gatcgtaaac cgtcacctct tcttcgtgat acaaacgcca caacacttcg tttgccggta
    23161 aggtcagcag ttcttcggtt ttgatggttt cggttagcgt cgccaggtgg tcaaagtcgt
    23221 cctgctgggt attttgcgca ggcattacct gcaacaacat accgcctgca gccggtttgc
    23281 cgtctacgtc gccggtgcga ataaacaggc gcgtcggcag ctgttcagaa cgcataaagt
    23341 aatcttctag gcaggccgcc agggtatcac cttccagacc aactacgccc tgatagcgtt
    23401 cgccttcgct cggggtaatg gtgatcacca cgtaaccatt gccgaccagc gttttcaggt
    23461 cggcattttc tggaatttcg ccctgcacgc gcgccacacc gcgcatttgc tggttattgt
    23521 taccgttaat aaccgccaga ttcatcggac cgtcgccctg cagctgtacg gtgatatcac
    23581 catcaaactt cagcgtagcg gttaacaggc tggtcgcaac cagcagttct gccagcacgt
    23641 ttttaacggg ctgcggataa tcgtggttct caaggatctg ttgcagggtt tccgaaacgg
    23701 ttaccagttc gccgcgaacg gcaaaatttt caaacagata gcgatgtaat tggtcatgtt
    23761 gcggcataat catctctctt gcaggtgaca gttattcact gtcgccgtgt ttaaatcgta
    23821 acaggtcgcg gcgctctttt ttgtccgggc gtcggtccgg gtgcggcatg gttaaggcat
    23881 taagtttacg tgccatcgcc attttttcgc gtttctctac actttccgca gtctcttcat
    23941 acagcaaggc tgcctcgctg gcggggcgac gctgttcagt aatcgccttt acaatcaccg
    24001 tgcgttcgtc atttccctgg cgcagagtga gcgtggcatt cagctcgacg attttgctcg
    24061 gcttgctgcg ctgcccgttg taatgcacct taccgccttt aatcatttca cgggccagcg
    24121 cgcgggtttt ataaaaacgg gcagcccata gccatttatc cagtcgaacc tcaacagcag
    24181 gtttctcttt catggcgtct ccttcacatt agcgagggga tcaggcggcg gtagtcattc
    24241 agtgacggat ggcgttgata ctgtttctcg gcaatcccgg aatcaggatt agtcacgccg
    24301 aggcagtaac gaataccaaa ttgcgcggca gcatcgagaa tcgcttcgct gtcatcaata
    24361 aacagcgttc tttcagcttt cagacccgta gcttcggcca ccgcatgcca taaccgctga
    24421 tcctctttcg gataaccaaa tgtgtgggtg gaaagtaata aatcaaggtg tgcgtccaga
    24481 cccgtatgct caagttttac cgccaggttg tgcggatgag cattggtgag caaaattcgc
    24541 tgcttaccgc tggctttcag tgcctcaaga aacggaatgg tatcttcacg cagtacggcg
    24601 cgcggtccca tctcggtggt catcgcacag atatccagac ccagttgctc actccagtaa
    24661 tcaagacagt accagtttag cgtatgctgt acgtcgtgat attgctggcg catatattcc
    24721 atcgcttcct gtggcgtaac cccgtttttc gcgccccatg tttcaggcac cagcttttgc
    24781 cagaaatagt tatcgaaggc gaggtcgagc aacgtgccgt ccatatccag cagaacggta
    24841 tctacgtcct gccaggcaat gttgatatgc atgagggaaa tctccagagt gaagcaattt
    24901 gcgcgacagg gtagcataac ctgccgcgca aacgtgttat tcgataaggc tttctgaagg
    24961 ggtgatcagt tgcgggttca ggcagctttc ataataggcc tgaatctcca tcatgcgagt
    25021 gcgatgacgc tggtaacgac gccaggcctg tacgccattg taaatggcgc tacccagcat
    25081 cgtcagtagc agcaatgtgg tgctgagata gcgccacagg ccggaacgat ccgggatcgg
    25141 atgaaggcca atatgctgcg taccattggc gtcagtgaag atttttgtga cgataccttc
    25201 ggcgttaaac ggcgtatgca tcagcatttg tgccagtttc tggaaagcgt tccactgttc
    25261 ttgcggcggg tagtcgtaaa gtgatgccga aggccagggc tgatcaacaa aatcgctgcc
    25321 ttcgtcgctg acaatcagga atccgccggg ggccggactg tttagtgatt gtgccgctcg
    25381 cgccgtttca tgcgtgataa acggcgcggt ggaggttgcc accaggttat ccagcgattc
    25441 cgcactcacc gggcgtaata acacattcac gccatcgagc ttcccagcgt tggcgcgttt
    25501 taccagcgcg tcccagtctt tactgttgcc gagattgacc agcgcatttt tcagtcgcac
    25561 acagtcatct ttggcagaac ataaatccgc tgtcttcagt acaatgtcgc caaaatcatc
    25621 aagcaatacc atgccggatt tttgaatcgc tgaacgtaat gaggcactga cgcgagattc
    25681 atcttccggt ttagggtgca gctggcgatt aactgcttca gtcaatgccg tcgctttgtt
    25741 gaccagttca gattctggta atggtaatga gcgggcgtca ttccagatga tctgcgagca
    25801 gtcaaatggt aaaaaaggtg aattggtttt cgcgctccag gttccggaag tgcgaatatt
    25861 acacattccc gtaccgctaa tacgcaatgt atcgcctacc cgcacgccag cgtcagccag
    25921 ttgttttacg ctggtggctt caatggtctg cgcgcctttc atccatgaga gagtgaattt
    25981 cagcggcata tccagcggga tccagaataa cagcataaac agcaccagca gcgagcccgc
    26041 agcgataatc gtactgcgca gccagtgctg taacggaaag ttttttactt catcatgcag
    26101 cgaaagatat cgcccctgac gcactacgtg gcggtc
//
LOCUS       DADMGC010000048        25989 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.048, whole genome shotgun sequence.
ACCESSION   DADMGC010000048 DADMGC010000000
VERSION     DADMGC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.048"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            106..1089
                     /locus_tag="JNZ73_001958"
     CDS             106..1089
                     /locus_tag="JNZ73_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY8732255.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            1112..2623
                     /locus_tag="JNZ73_001959"
     CDS             1112..2623
                     /locus_tag="JNZ73_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY8732256.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2648..3646
                     /locus_tag="JNZ73_001960"
     CDS             2648..3646
                     /locus_tag="JNZ73_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8732257.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNAQPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3679..4773
                     /locus_tag="JNZ73_001961"
     CDS             3679..4773
                     /locus_tag="JNZ73_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAY8732258.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGYRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGAVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            4785..5657
                     /locus_tag="JNZ73_001962"
     CDS             4785..5657
                     /locus_tag="JNZ73_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAY8732259.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5705..6127)
                     /locus_tag="JNZ73_001963"
     CDS             complement(5705..6127)
                     /locus_tag="JNZ73_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY8732260.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6224..7426)
                     /locus_tag="JNZ73_001964"
                     /pseudo
     CDS             complement(6224..7426)
                     /locus_tag="JNZ73_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(7436..8248)
                     /gene="hcaB"
                     /locus_tag="JNZ73_001965"
     CDS             complement(7436..8248)
                     /gene="hcaB"
                     /locus_tag="JNZ73_001965"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(cis-5,6-dihydroxycyclohexa-1,
                     3-dien-1-yl)propanoate dehydrogenase"
                     /protein_id="HAY8732261.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVAALELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(8245..8565)
                     /locus_tag="JNZ73_001966"
     CDS             complement(8245..8565)
                     /locus_tag="JNZ73_001966"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HAY8732262.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(8565..9083)
                     /locus_tag="JNZ73_001967"
     CDS             complement(8565..9083)
                     /locus_tag="JNZ73_001967"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HAY8732263.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(9080..10441)
                     /locus_tag="JNZ73_001968"
     CDS             complement(9080..10441)
                     /locus_tag="JNZ73_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HAY8732264.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            10577..11467
                     /gene="hcaR"
                     /locus_tag="JNZ73_001969"
     CDS             10577..11467
                     /gene="hcaR"
                     /locus_tag="JNZ73_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HAY8732265.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            11627..12766
                     /locus_tag="JNZ73_001970"
     CDS             11627..12766
                     /locus_tag="JNZ73_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HAY8732266.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(12758..14038)
                     /gene="csiE"
                     /locus_tag="JNZ73_001971"
     CDS             complement(12758..14038)
                     /gene="csiE"
                     /locus_tag="JNZ73_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HAY8732267.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRITRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTPQQEQIRKMLESA"
     gene            complement(14229..15083)
                     /locus_tag="JNZ73_001972"
     CDS             complement(14229..15083)
                     /locus_tag="JNZ73_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY8732268.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVLRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMLPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            complement(15228..16031)
                     /gene="suhB"
                     /locus_tag="JNZ73_001973"
     CDS             complement(15228..16031)
                     /gene="suhB"
                     /locus_tag="JNZ73_001973"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HAY8732269.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGDL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKR"
     gene            16150..16890
                     /gene="trmJ"
                     /locus_tag="JNZ73_001974"
     CDS             16150..16890
                     /gene="trmJ"
                     /locus_tag="JNZ73_001974"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HAY8732270.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            17160..17648
                     /gene="iscR"
                     /locus_tag="JNZ73_001975"
     CDS             17160..17648
                     /gene="iscR"
                     /locus_tag="JNZ73_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HAY8732271.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            17760..18974
                     /gene="iscS"
                     /locus_tag="JNZ73_001976"
     CDS             17760..18974
                     /gene="iscS"
                     /locus_tag="JNZ73_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAY8732272.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            19002..19388
                     /gene="iscU"
                     /locus_tag="JNZ73_001977"
     CDS             19002..19388
                     /gene="iscU"
                     /locus_tag="JNZ73_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HAY8732273.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            19405..19728
                     /gene="iscA"
                     /locus_tag="JNZ73_001978"
     CDS             19405..19728
                     /gene="iscA"
                     /locus_tag="JNZ73_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HAY8732274.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            19824..20339
                     /gene="hscB"
                     /locus_tag="JNZ73_001979"
     CDS             19824..20339
                     /gene="hscB"
                     /locus_tag="JNZ73_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HAY8732275.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            20356..22206
                     /gene="hscA"
                     /locus_tag="JNZ73_001980"
     CDS             20356..22206
                     /gene="hscA"
                     /locus_tag="JNZ73_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HAY8732276.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            22208..22543
                     /gene="fdx"
                     /locus_tag="JNZ73_001981"
     CDS             22208..22543
                     /gene="fdx"
                     /locus_tag="JNZ73_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HAY8732277.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            22555..22755
                     /gene="iscX"
                     /locus_tag="JNZ73_001982"
     CDS             22555..22755
                     /gene="iscX"
                     /locus_tag="JNZ73_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HAY8732278.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            22814..24097
                     /gene="pepB"
                     /locus_tag="JNZ73_001983"
     CDS             22814..24097
                     /gene="pepB"
                     /locus_tag="JNZ73_001983"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HAY8732279.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKRVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            24239..25015
                     /gene="sseB"
                     /locus_tag="JNZ73_001984"
     CDS             24239..25015
                     /gene="sseB"
                     /locus_tag="JNZ73_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HAY8732280.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDCALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25510..>25989)
                     /gene="sseA"
                     /locus_tag="JNZ73_001985"
     CDS             complement(25510..>25989)
                     /gene="sseA"
                     /locus_tag="JNZ73_001985"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HAY8732281.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
BASE COUNT         6146 a         6500 c         7217 g         6126 t
ORIGIN      
        1 gttcactaaa ccctattaac tgtaatattt gagcggcacg cgctgccgct catcacaaaa
       61 taattagaat aatccgttgt taataaaaac cctacaggag gcactatgcc taaaaaaatg
      121 agaacaaccc gtaatctatt attaatggct acgctattag gtagcgcact atttgccagg
      181 gctgcggata aagaaatgac cattggtgca atataccttg atacccaggg atattacgct
      241 ggagtgcgtc agggcgttca ggatgcggca aaagattctt cagtacaggt acagttaatt
      301 gaaactaacg cccaggggga tatttcgaaa gaaagtacct ttgttgatac cctcgtggcg
      361 cgtaatgtcg atgccattat tttatcggca gtgtctgaaa atggcagtag ccgtaccgtt
      421 cgtcgcgcca gtgaagcggg tattccggtg atttgctaca acacctgtat taatcaaaag
      481 ggtgtcgata aatatgtctc ggcgtatctg gtcggcgatc cactggaatt tggtaaaaaa
      541 ctgggtaacg ctgccgccga ttattttatt gccaataaaa ttgaccagcc gaaaattgcc
      601 gtcatcaatt gcgaagcctt tgaagtttgt gtgcagcgac gtaaaggatt tgaagaagta
      661 ttaaaatccc gcgttcccgg cgcgcaaatt gtcgctaacc aagaagggac tgttttagat
      721 aaagcgattt ccgttggtga aaaactgatt atctccacgc cggatctcaa cgccattatg
      781 ggggagtcgg gcggtgcgac actcggcgcg gtaaaagcgg tacgtaatca aaatcaggcc
      841 ggaaaaattg ctgttttcgg ttcggatatg acaaccgaaa ttgctcagga gctggaaaac
      901 aatcaggtgc tgaaagcggt agtggatatt tccggtaaga aaatgggcaa tgctgttttc
      961 gcgcaaacat tgaaggttat caataaacaa gccgacggtg aaaaagtgat tcaggtgcca
     1021 atcgatctct ataccaaaac ggaagatggt aaacagtggc tggcaacgca cgttgatggt
     1081 ctgccctaat cgctgattcg cagaggtccc tatgttcacg gcaacagagg cagtcccggt
     1141 agcaaaagtg gtggcaggaa ataagcgtta ccccggcgtc gttgcgctgg ataacgttaa
     1201 cttcacgctc aataaaggcg aagttcgtgc gctgttaggt aaaaacggcg cgggcaaatc
     1261 gactctcatt cgaatgctta ccggcagcga acgcccggat agcggtgata tctggattgg
     1321 cgagacgcga ctggaaggtg acgaagctac gttgactcgc cgtgccgctg aactgggggt
     1381 tcgtgcggtt tatcaggaat taagtctggt ggaagggctg acggtggcag aaaatctctg
     1441 ccttggtcaa tggcctcgcc gcaacggcat gattgattac ctgcaaatgg cgcaggatgc
     1501 ccaacgttgc ttacaggcgc tgggcgttga cgttagtcct gaacaacttg tttcaacgct
     1561 aagcccggcg caaaagcaac tggtggaaat tgcacgggtg atgaagggcg agccgcgcgt
     1621 ggtcattctt gatgaaccca ccagctcgct tgccagtgca gaagttgaac tggtgatcag
     1681 tgcggtgaaa aagatgtcgg cactgggcgt ggcggtgatt tatgtcagcc accggatgga
     1741 agaaatccgc cgcattgcct cctgtgccac cgttatgcgc gatggtcagg tggcgggcga
     1801 tgtgatgctc gaaaacacct ccacgcatca tattgtgtcg ctaatgctag ggcgtgatca
     1861 cgttgatatt gcgccggttg cccctcagga aattgtggat caggccgtac tggaagtccg
     1921 tgcgttacgc cataagccca agctggagga tatcagcttt acgctacgtc gtggcgaagt
     1981 gcttggcatt gctggcctgc tgggggcagg gcgcagtgaa ttgctgaagg cgattgttgg
     2041 gctggagacg tatgaacagg gcgaaattgt tatcaacggc gagaaaatca tgcgccccga
     2101 ttacggcgac atgctgaaac gcggcattgg ctatacgcca gaaaaccgca aagaagcggg
     2161 gatcattccc tggctgggcg ttgacgaaaa tacagtgctg accaatcggc aaaaaatcag
     2221 caccaacggt gtgctgcaat ggtccaccat ccgccgcctg accgaagagg tgatgcagcg
     2281 gatgacggtc aaggctgcca gtagcgaaac acccatcggc acgctttctg gtggcaatca
     2341 gcaaaaagtg gtgatcggtc gttgggtcta tgccgccagc cagattttgt tgctcgacga
     2401 gccaacgcgc ggcgtcgata tcgaagccaa acagcagatt taccgtattg tccgtgagct
     2461 ggctgccgaa ggaaaaagcg tggtgtttat ctccagtgaa gtggaggaat tgccgctggt
     2521 gtgtgaccgc atcctgttat tacagcacgg cacgttctcg caggagtttc actcgccggt
     2581 caatgtggat gagctgatgt ccgccattct gtctgtgcac tgatgaattt taccgaggaa
     2641 cctgaagatg tctgcttcgt cattaccatt gccgcagggc aagagcgtct cgctcaaaca
     2701 atttgtcagt cgccatatta atgagatcgg tttgctggtg gtgattgcca ttctttatct
     2761 ggtcttctcc ctgaacgcgc ctggctttat ctcattgaat aaccagatga acgtgctgcg
     2821 cgatgccgcc accattggga ttgccgcctg ggcgatgacg ctgattatta tctccggtga
     2881 aattgatgtc agcgttgggc cgatggtggc ttttgtctcg gtgtgcctgg catttttgct
     2941 gcaatttgaa gttccgttgg cgattgcttg tctgctggtg ttgctgttag gcgcgctgat
     3001 gggaacgctc gccggggtgc tgcgcggcgt gtttaacgta ccaagtttcg ttgccacact
     3061 ggggctgtgg agcgctctgc gcggaatggg gctgtttatg accaacgcac agccagtgcc
     3121 gattgacgaa aacgaggtgc tggactggct gggcggacaa tttctcggtg tgccagtatc
     3181 cgcgctgatc atgatggtgt tgtttgcgct gtttgtgttc attagccgaa aaaccgcctt
     3241 cgggcgctcg gtttttgccg ttggcggtaa tgccacggcg gcgcagttgt gcggtattaa
     3301 cgttcgtcga gtgcgcattc ttatctttac cctttcggga ttattagcgg cggtgaccgg
     3361 cattttgttg gcggcacgcc tcggttccgg taacgcaggt gccgcaaacg gtctggagtt
     3421 tgacgtcatc gccgcggtcg tggtcggcgg tacggcactt tccggcggtc gcggttccct
     3481 gttcggtaca ttgcttggtg tgctggtgat tacgctaatc ggtaacggtc tggtgctgct
     3541 cggtattaac tcctttttcc agcaggtggt acgcggcgtc atcatcgtgg tggcggtgct
     3601 ggcgaatatc ttgctgaccc agcgaagcag taaagcgaaa cgctaaacct taacgcccct
     3661 ggcgcgggtg cggcagggat gaaaattaat cgtattgtta atgagggatt tatgaaaacg
     3721 atgctggcag cttatttacc aggaaattca accgtcgatc tgcgggaagt tgcggtgccg
     3781 acgccgggga ttaaccaggt actgatcaaa atgaaatcct ccgggatttg cggaagcgat
     3841 gtccactata tctatcatca acaccgtgcc acggcggcgg cacccgataa accgttatac
     3901 cagggattta tcaacggtca tgaaccgtgc gggcagattg tggcgatggg gcaaggctac
     3961 cgccatttta aagagggcga ccgcgtgctg gtgtatcaca tttctggctg tggtttttgc
     4021 ccgaactgcc gccgcggctt tcctatctct tgtactggca aaggaaaagc ggcttacggc
     4081 tggcagcgtg acggcggtca tgccgaatat ctgctggcgg aagaaaaaga tctgatcctc
     4141 ctgccggatg cgctgagcta cgaagatggt gcgtttatca gttgcggcgt tggcacggcc
     4201 tatgaaggga ttttgcgcgg cgaagtttcc ggcagcgata acgtgctggt ggtcggtctg
     4261 ggggcggtcg gcatgatggc gatgatgctg gcgaaaggtc gcggtgcaaa acggatcatc
     4321 ggcgttgata tgctgccgga acgtctggcg atggcaaaac agttaggggg gatggatcac
     4381 ggctatttag caaccaccga aggtctgccg cagataattg ctgaacttac ccacggtggc
     4441 gcggatgttg cgctcgattg ttccggtaat gccgcaggtc gcttgctggc actgcaatcc
     4501 actgctgact gggggcgggt ggtttacatt ggtgaaaccg gaaaagtgga attcgaggtc
     4561 agcgctgatc tgatgcacca tcaacggcgg attatcggct cctgggtgac cagtctgttc
     4621 catatggaaa aatgcgccca cgatttgact gactggaagc tgtggccgcg taacgccatt
     4681 acccatcgct tctcgctaga acaggcaggt gatgcctatg tgctgatggc gagcggcaaa
     4741 tgcgggaaag ttgtgattaa cttcccggat taaggagtta gcggatgacc atctatacct
     4801 tatcccacgg gtcgctaaag ctggacgttt ctgaccaggg cggcgtaatc gaaggcttct
     4861 ggcgcgatac gacgccgctc ttacgtcctg gtaaaaaaag cggtgtggca accgatgcct
     4921 catgttttcc gctggtgcca tttgcaaacc gggtgagcgg caaccgtttt gtctggcagg
     4981 ggcgtgagta tcaactgcaa ccgaatgttg agtgggatgc gcactatcta cacggcgatg
     5041 gctggctggg cgagtggcaa tgtgtctcgc acagcgatga tagtttgtgt ctggtgtatg
     5101 aacatcgcag cggtgtctat cactatcggg taagtcaggc gtttcattta acggcggata
     5161 cgctgacggt gacgctctct gtcaccaatc aaggggcaga gacgctgcca tttggtaccg
     5221 gctggcatcc ttattttccg ttgtcaccac aaacgcggat tcaggcgcag gcgagcggtt
     5281 actggctgga acgggagcag tggctggcgg gtgagttttg cgagcagcta ccgcaggaac
     5341 tggattttaa ccagctcgcg ccgttgccgc gtcagtgggt gaacaatggt tttgccggat
     5401 ggaatggtca ggcccgcatc gagcagccgc aggaaggtta tgccatcatc atggaaacga
     5461 cgccacctgc accgtgttat ttcatctttg tttccgaccc tgcgtttgat aaaggatatg
     5521 cgtttgattt cttctgtctg gaaccgatga gccatgcgcc ggatgatcat catcgcccgg
     5581 aagggggtga tctcattgcg ctagcaccag gggaatcaac aacctcagag atgtcgttgc
     5641 gggtagagtg gctgtaattt ataaaatgcc ggataaggcg ttcacgccgc atccggcatc
     5701 tttcctaacg ccgatcgaga gaaattgccc ccggtccggt aattgccaat agcaagaacg
     5761 cgccagcgat actgacattc ttccagaaat taatcatatt tggcccaacc gcatcgccgg
     5821 tcatatccca gtaatggtga ccaatcaccg ccgtacccag cgtgtagaaa ataaacagca
     5881 ccgccagcgg acgggtgaaa aagccaagca cgattaatat cgcggcgggc acttccataa
     5941 ctaccgcaat aatcgctgcc agcatcggca ttggcgcgcc caacgaggcc atatattgga
     6001 ccgtaccgtc aaagcccatc attttgggaa aaccaaaaat aatgaaaatt aagaccacgg
     6061 cgatacgggc aattaataac aaaacggggc gggcagctcc aaaatcaaaa taacgtagtg
     6121 tgttcataac caatcccttt gcaagataat gtgatttaaa cgtaatacac actcttgcac
     6181 ctcgccacgc cccaggcatt gagatattcg ctaattatcc tggttacagt gatttaagcg
     6241 cgatgttctc atctatcagc agtttcgcat caaacgtttt gccgctctgg atccatttgc
     6301 gaattgggcg aatctcacgc ccctgattca gcgtgaccgc accgataagc acgccgtttt
     6361 gcagattaaa ccaaatcgcc ttctgagttt ctgggttgcc acgacaaagc tagtcatcgc
     6421 cacgcatatc gccaataaac tgtaagttat cactgtactg atcgctccag aaccacggcg
     6481 gcggcagtag cggtagcggt agccctaaca ttgcggcagc ggcaatttgc gcctggttat
     6541 tggcgttttc ccagctttcg cagcggtgta gtgcaccatt atcaagacga gtgattgcca
     6601 catcgccacc ggcaaagatc gcgggatcgc aggtgcggca agcctcatca atgacaatgc
     6661 cattggcagt atcaaggttg gcctcgcgag ccagttgctc gttggcgctg atactaatac
     6721 cgtaaatcac cacatcagcc tgaagcgttt ccccactttg cagcgtcagt tctacttttt
     6781 caccatcgac cacatgttca atggcattat tgagcagaat gcgcacacca gcctgctgat
     6841 ggcgctgtaa aagatagcgt tgcacgggcg gtggtgcatt acggcccatg acggttgccg
     6901 ccagttcaat cactgtcacc ttacatctgc gctgcgtggc gctggcagcc agttccagac
     6961 caatagttcc ggcaccgaca atcacgactg accgttcggg ctgcagaact tctcgcagtc
     7021 tggcggcatc accggcatgg tgtagggtaa agcagcgttc tcccagtgca tcaagcaacg
     7081 gcagcggtcg agctgccgcg ccggttgcta taaaaagctg atcccagtgc cagctttcgc
     7141 cgttggttaa cactaactct cgtgtgtcgc gacccagcgt tttgatggtt acaccggaat
     7201 gcagatgaac attgttttcc tgccaccagt tagcgggtaa cacctgctgt aactgcgggg
     7261 aatcttccag caacatggat ttcgagagtg gaggtcgttc ataaggaaga tgtcgctcat
     7321 cggaaaacag atgcagctca ccggtgaacc cttgctggcg tagcgaggcc gcagccattg
     7381 ccgccgcttg cccgccaccg acaataatga tcgttttttc tttcatcgtg cttccttaaa
     7441 gatccagccc agccgctacg tggcgaatgc cgcgaatcgc taaacccgca tcagcgttga
     7501 tcatcacacc gcttaatgcg cgattattgc gccgcgatgc caacatcaca tacggccccg
     7561 taaaatccgc cggttgcggg aaaaattgca gcggtaaaat ggcggcaatt ttctccggcg
     7621 tcagagactg cattatcgag gtttcacttt gcccgagcgc ctgtgggccg cgcaggtcgc
     7681 tggccatacc acacgggccg acgccattca cccgcacttt cggtgccagt tcataagcca
     7741 gttggcgaat aagtccggtt gcggcatgtt tactggtggt gtacagcggg ccaccgccgc
     7801 caggatacca ggcggcattt gacagtgtga aaatcatgct gccttcactg gcgattaacg
     7861 ccggagcgca ggcttttgcg cccagcaggt aaccgagtac gttgacgtta aacagctcgt
     7921 ggaagccggt ttcgagcgtc tctgcgggag tattaaccag tgaggcattg tgatcccaga
     7981 tgcctgcatt gccgataaaa caatccagct tgccggaacg agtcaggatc tgatcgaccg
     8041 cgcgttgata atcggcataa caggtcacgt taccttccac cgccagaata tgttcgccaa
     8101 atcgctgacg cagactggcg acttttgccg ccgacagttc cagcgcggca acctgcgcgc
     8161 cttcttcgat aaatcgctcg accagcgcca gccctaatcc cgatccgccg ccggtaataa
     8221 aaatggactc gttatgcaga tcgctcatgg ctgcgcctcc ggtaagtcga tgaaaatgtc
     8281 accaccttca acgtgtactg gataagtggt gagcggatcg gtggcgggca ggcataacgc
     8341 tttccccgtt ttcaggcaaa aactggcggc gtgtagcggg cactccaccg tggcgtcatc
     8401 ttccagatac ccttctgaca tcgacgcatt accatggctg caacgatcgt taatggcata
     8461 aaactcgccg cccacgttga acaaggcgat gacgggcgag gtatctatcc ggagagcctc
     8521 accctccggc acatccgcaa cgggacacgc ataaattcga ttcatcagaa cagtacactc
     8581 aggttatggg aagtgatcac cgcttgatcc aggacgatat cccgttccag caagcgccag
     8641 ttgtcatctt ccagacggcg aactttgtcg aaacgcgttc caacatagaa tgtttcatcg
     8701 cgctcttttt gtgcccgata aagtagataa ttcaccctca cggcaaatac gtttgggatg
     8761 tcggtttcgc ttacctggca gttgctgatt aagtgacgag tgcgtgacgg cggctcttct
     8821 gcccaggcca tgcccgtttc cagacgggcg attcgccgct ccagctggtc tttggtgtca
     8881 ttgaaaatcc aggtcgtcgg cggctggacg cctttgcgac ggtcgcgtgt ttgcgcgtta
     8941 accgtggtgc gcatggtgta acgaatctct tcgtcgagct gtgccaacca gtcacgaaat
     9001 ttccagtcat ccagcagact ggcttcgtga aagagaaact ggctaatgcg gtgatgtaac
     9061 tccagtgaaa cttgcgcact catttcatca cctcctgctg gtaagcggcg gttttatcga
     9121 gcacttcctg ccagctttcg ctactcagaa gatcggccca gcgttggtac attccacgag
     9181 cggcagtttc tgaaaagata tagttagtaa tgccaggaat gccgtcgtcg cggcgctttt
     9241 cctgaccaag ccccatttcc agacacagtt tgctgttgcg ggcgcggtgg cctttaagca
     9301 atttctggat ttcacaccag ttctccgagt catcctgctc gagaaaacca gcaggaccaa
     9361 aagcacgagt ggcgctgttt tcaaaagcgg ctttaacttc atcggaggcg gctttgtcag
     9421 taatacagaa cgcccacact tcaacttgat cagggccgcg cggatgccag acgcggagtg
     9481 tggcagtgcc gtttagccat gaaagcgtgg ggaatatatt gttatgaccc gccaggcgca
     9541 gggcgcgaac ttcacctaaa cgttgttctg cttcggcata ggtttcgcgg taatagcttg
     9601 aaactgcgcc atcgacccag acattagcat ccggtttttc agtaaagaag aaaccgctac
     9661 cgtgaccgtc ctgaccaaat tgcagcgcat ctttggcggt ttcccacacc gggcgggcgg
     9721 tttgtccatc accgaggcgc ttatcgctgc catcatcttt cgcccctaat acctgaacgg
     9781 cagaagcatg gctgaacaga gcatgatact ggtcactggc gaactgctct gccgggaatt
     9841 tccagttaca gttgatcacc cacttttgta cgccgccgac aatttcggtg ccgccttcgc
     9901 gacgatccag catgccatcc agataccagg caatgtcacc caggtaatca tgcaggcccg
     9961 gtgcgctggt atcccagttg ccaaaaatca gtcctttata actctccaca caaggaactt
    10021 cgtttagccc ccagtgggat ttacacaacc cttgtgggta ggcgcgaggt tccagcggta
    10081 catcgatcaa ctcgccgtta atgccataag accagccgtg atacgggcag gtaaaggcgc
    10141 gagtgttgcc acaatctgca taactcacac gcatggcccg gtggcggcat tggttaagaa
    10201 acgccttgat gctgccgtct ttctggcgca ccacgacaac tgcatcttct cccatgtagg
    10261 tgttaaagaa atcaccgggt tttgggatct ggctttcgtg ggcgagaaat agccagcaac
    10321 gaccgaaaat acgctcaagc tccagttggt aaatgtccgg gtcggtataa atacgcggag
    10381 tgacccgacc gttttgggtg tcaatcagtt ggtaaatgtt caaatctgag ggtgtggtca
    10441 tattttttaa tcctgaaaga taagatgtat tgaccttcta tttttgtgcc ataaggatgt
    10501 tgctggttgg ttgctaatat gtgaaatagt taatttctac cagctgagac gttttttcga
    10561 taacaagggg aaggtgatgg aactacggca tttacgctat ttcgtcgcag tggcgcaggc
    10621 actgaacttt acccgtgcgg cggaaaaact gcatacctca cagccttcgt taagcagcca
    10681 gatccgcgat cttgaaaact gtgtcggtgt tccgttactg gtgagggata agcgcaaagt
    10741 cgcgttgacg gcggcgggag aatgttttct ccaggacgcg ctggcaatcc tcgaacaagc
    10801 ggaaaatgcc aaattacggg cgcggaaaat tgttcaggaa gacagacaat taaccattgg
    10861 ctttgtgcca tcggcggaag tgaatttatt gccaaaagta ttaccgatgt ttcgtctcag
    10921 acagccagac accttgattg agctggtgag tttaatcacc acgcaacagg aggaaaaaat
    10981 tcgccgtggt gaactcgacg tcggcttaat gcgccatcct gtttatagcc cggagattga
    11041 ttatctggag ctttttgacg aaccattagt ggtggtgtta ccggttgatc accctttagc
    11101 acatgaaaaa gagatcaccg ccgcgcaact ggatggcgtg aatttcgtca gtaccgatcc
    11161 ggcgtattcc ggttcgcttg cgccgatcgt taaagcgtgg tttgcgcaag aaaacagcca
    11221 gccaaatatc gtccaggtgg caacgaatat tctggtgacc atgaatctgg tgggcatggg
    11281 gctgggcgtc actttgatac ccggttatat gaataatttt aataccgggc aggttgtttt
    11341 tcgtcccatt gccggaaatg tgccttccat tgctttactg atggcgtgga agaaagggga
    11401 gatgaagccg gcattacgcg atttcatcgc cattgtgcag gaacgtttgg caagcgtaac
    11461 ggcataatta cgcattattt ttgcaacatg cgccgcggat cgcagttttt tagtgcttgt
    11521 gaactggcgt tacagacatc gggaagcagg ctcgaatatt cttatttttc aacttgcacc
    11581 aacattcgat taaccccaga ctaacgcctc ctgacgggag ggactcatgg ttttgcaatc
    11641 cacgcgctgg ttggcgctcg gctatttcac atactttttt agttacggca tttttttacc
    11701 tttctggagc gtctggctta aagggattgg tttaacgcca gaaaccatcg gcctgttatt
    11761 gggggcaggt ctggttgccc gttttctcgg gagtttgctc atcgcgcccc gcgtcagcga
    11821 tccttcccgc ctgatttccg ccttgcgcgt gctggcactg ctgccacttc tctttgctgt
    11881 cgccttctgg gcgggggcgc acgtagcgtg gctgatgctg gtgatgattg gctttaacct
    11941 ctttttctca ccgctggtac cgttgaccga tgcactggcg aatacgtggc aaaagcagtt
    12001 cccgcttgat tacggcaaag tgcgactgtg gggctcggtg gcgtttgtca ttggctcggc
    12061 gctgacgggc aaactggtca ctatgtttga ttatcgggtg atcctcgtgc tgttgacgtt
    12121 gggcgtggca tccatgctgc tcggctttct catccgtccg acgattcagc cacaaggggc
    12181 aagccgccag caggagagca ccggttggtc tgcgtggttg gcgctggttc gccagaactg
    12241 gcgctttctg gcctgcgttt gtttattgca gggggcacat gcggcctatt acggttttag
    12301 cgccatttac tggcaggcag ctggctactc ggcctcggcg gtggggtatt tgtggtcgct
    12361 gggcgtggtg gcggaagtca ttatctttgc gctgagtaat aaacttttcc gccgttgtag
    12421 tgctcgcgat atgctgttga tctcggcgat ttgcggcgta gtgcgctggg gcattatggg
    12481 agcaactacg gcgttgccgt ggttgatagt ggtgcaaatt ctgcattgcg gcaccttcac
    12541 ggtctgccac ctggccgcca tgcgttatat tgctgctcgc caggttagcg aagtcatccg
    12601 tttacaggcg gtttactctg ccgtcgcgat gggcggcagt atcgctatca tgaccgtttt
    12661 cgccggtttc ctgtatcaat atctgggcca cggtgtgttc tgggtaatgg cgctggtggc
    12721 gcttccggca atgtttttgc gcccgaaagt tgttccctca tgctgattcg agcattttgc
    12781 ggatctgctc ctgttgaggt gttgtcagcg tcaggtccgt atagatcagc ggtggtgaaa
    12841 agagcggtaa cggcatggta taaggcgcaa taatcagtgc cgcgccacgc ggagcgcctg
    12901 tctgcaaaaa tgcctttacc gacatatgct taatgttgag cggcagtaac gttagttcgc
    12961 gtaactgctg ctcaatctgc gcttctcgct cgctatcatt cccggtcagt agaataatct
    13021 gtttttcatg cagatcgttt tcctgcatta gccaggcacc gaaaatcacc gcgaccagac
    13081 cactttcctc atcagaaaga tggacgccgt attcactttc aaatccggcc agcgccgcgc
    13141 gggtgatgcg taccaggcgt ggatacagtc tggcgaactc ttccggcagg gtattatcaa
    13201 taccgatggc aaacaaactg cgatttaacg cctgggcgag gtggatataa agctgatcac
    13261 ataacccctg ttcatcataa aaacgaacat ttcccagctc acgaaaatgg tttaccagac
    13321 gtttgataga ctgacgcaat tgtctgtccc gctgatgcgc atcccgcagt ggatcgggaa
    13381 cccgcaacat ggaaaaaagt aatgccataa acagtggctc atcaggcggt acatgctgga
    13441 gcgcccgacg ctgccagtgg cgtccaattt cctgcgctac ctgaaattca aggcaggatt
    13501 ccgcccagcg acgttggagc ggattaaact gaggcgtaat tccagcgtgg tgctgcagca
    13561 aacaatattg cagatacaga cacaggaaat gaatatcgcg cgattcaaag cgtttttgca
    13621 gccgtcggga gcagagattc actaacgctt gtagattggt atcgtcgtaa aagttacgcg
    13681 caattccgcg ccgcttcagt tcactcttca gggcgggggt gaattggctg gtaataaatg
    13741 acggacacag acggaaacca cgtcgtagcc agtgaaataa acacaaacgt tggttaagca
    13801 ctgtaccttc aacccggtag ctaccgtcat aaccagtcgt cagtgtgagt tgatgatagc
    13861 gcaggatctc ccgtcctgtt tcgctgatat caagactggc aatatcatca tccacaccat
    13921 taagctcgct gaaggttgcc atggtggcgg tcaaccccgg ctgaaagagc gtcagcaaga
    13981 tctggcagcg gcgctgggga gccgaaagga cagatggtgg agcaagcgta ggcatcatct
    14041 gtagatctcc cataagcata attgataaga atagctaaag tttgctcagg gaaatggcga
    14101 agggaatgct aatcatcaga aatgttggca agcgtcacag aatttttctc attttaaact
    14161 gttctgacca gtggattagc atgtcttaag gtggttttgt gaatgattat cgtgctagcg
    14221 atcatgggtt agttctgcgg attcaacgcg ctaaggataa agtccaccat ctgttcgcga
    14281 taaacattgc cgtgacgatc ggaaaaagca tcaatgtgtt cgccatccgg gattaggatc
    14341 aaccgttttg gctcttttgc caggctatac aacttttcgc tgtgctgcca tgggataacg
    14401 tgatcggctt taccgtgaat gagtaaaagc gggatcgggc tgacgctggc gatgtaattt
    14461 tcgccgctgt aactctcatc aagtaagtag ccactgccgg ggagcatctg gttggcgatg
    14521 gttgcataag aggcaaatgt ggagtcgagg atcaccgcac gtatgccttc acgatcaccc
    14581 tgaccaataa cagcaagaat attcgccccg ccaatactct gcccgaacag caccagccgt
    14641 tgtgggttta catcactgcg atggcgcacc acattgatgg cgctttgcgt atcgtccagc
    14701 aatccggcct gggacggagt gccttttgat ttaccaaacc cgcgataatc aaacataaaa
    14761 acgttgaaat tacgctcagg taaccaactg accagcggcc agtgggcgga catatttccg
    14821 gcattgccgt gagcatgaat gatggttgcg atggcgttgt cagcagggcc cgtcgaagaa
    14881 ggaataaacc agccttgcag acgagtaccg tccttagccg taaattcgac ggattccgcc
    14941 gaccaggggt cgggaccgta aattttatcg tcaggataat agaagaagtt gatggcaacg
    15001 cgcagcacta aataaacgca gaaggcaaca acaaagagaa taaacagaat tttgggaacg
    15061 cgtttgttca ctggcagtgc cataaccttt ccttgcgact tctttatcat ccattaatcg
    15121 gggaaagatg gtgggagagt gacgaaagaa agtaaagcgg tgaggaagta tttcggaata
    15181 gcgcctgaaa ggcgagggcg ggtgagtgat atcacccgcc tgagtcatta acgcttcaga
    15241 gcgtcgctta actcgtcacg catgttcgcc agtatggctt tcacaacacg cgggttacca
    15301 gcaacgatgt taccggtcag catatagtta tgaccaccgg tgaagtcgct gacgatgccg
    15361 cccgcttcac gaaccagcag atcgcctgcc gcgaaatccc acggacgcag accgatttca
    15421 aagaaaccgt caacacgacc cgcagcgacg taagccagat ccagcgccgc agaaccggta
    15481 cgacggaagt ctgcacattc gttgaacagc ttgccgacga tgttgatgta ggtagtggcg
    15541 tactgttttg ctttgaacgg gaagccggtc gccagaatgg taccgtcgag atcgcgagcg
    15601 gtgctaccgc gcagacggta gccgttcagc tgtgcgccct gaccgcgagt ggcggtgaac
    15661 agttcgttac gcataggatc gtataccaca gcaacttcgg tgcggccttt gatacgaacg
    15721 gcgatagata ccgcgaagtg cggcagacgt ttgataaagt tggtagtacc atccagtgga
    15781 tcgataaccc attgaacatc ctgatcagta ccttcaagtt caccgctttc ttcggtgatg
    15841 atggtgtgct gtgggtaaga tttacgaatc gtgtcgataa tcaccgcttc ggcagcttta
    15901 tctacgttgg tcacgaaatc gttactgcct ttctggctcg cttctacagc gtccggggtt
    15961 tcatagtttt tggcaattaa attacccgcc ttgcgcgctg cgcgcacggc gatgttcagc
    16021 atcggatgca tcagtctctc tcactggatg ttaaagaacg ggaaaacggc gcagagtata
    16081 gcagcgagat cggcatctgt cttcgtttta tgataagatg cgtgaataat ctttagatga
    16141 agaaagacaa tgctgcaaaa tattcgaatt gtgctggtgg agacgtcaca caccggcaat
    16201 atgggttctg ttgcccgtgc catgaaaaca atgggattaa ccaatctgtg gctggttaat
    16261 ccactggtga aacccgactc ccaggcgatt gccctggctg caggggccag cgatgtgatt
    16321 ggtaatgctc acatcgtcga tacgctcgac gaagcgttag ccggttgtag cctggtggtg
    16381 ggcaccagcg cacgctcccg cacgctgccg tggccgatgc tcgacccgcg cgaatgcggt
    16441 ctgaaaagcg tcgctgaagc ggcaaatacc ccggtggcgc tggtgtttgg tcgtgagcgc
    16501 gtcggcttga ccaatgaaga gttgcagaaa tgccattatc atgtcgcgat tgcggcaaac
    16561 ccggaataca gttcgctgaa cctggcaatg gcggttcagg ttattgccta tgaagtgcgt
    16621 atggcctggc tggcaactca ggaaaatggc gagcaggtcg agcatgaaga gacgccgtat
    16681 ccgctggtcg atgatctgga gcgtttttat ggtcatctgg aacaaacgct gctggcaacc
    16741 ggttttatcc gtgaaaacca tccggggcag gtgatgaata aattgcgccg tctgtttacc
    16801 cgtgcgcgcc cggaaagcca ggaattgaat atcctgcgcg ggattctggc ttctattgag
    16861 cagcagaata aaggtaacaa ggccgaataa cagtggttgc ctgatgcgac gcttaacgcg
    16921 tcttatcagg cctacaatgt acagaatcgt aggtcggata aggtgttcac accgcatccg
    16981 acaacaggta caaacgccac gataaaaaaa tggcactgaa ggttaaatac ccgactaaat
    17041 cagtcaagta aatagttgac caatttactc gggaatgtca gacttgaccc tgctatgcaa
    17101 tacccccact tttacaataa aaaaccccgg gcaggggcga gtttgaggtg aagtaagaca
    17161 tgagactgac atctaaaggg cgctatgccg tgaccgcaat gcttgacgtt gcgctcaact
    17221 ctgaagcggg cccggtaccg ttggctgata tttccgaacg tcagggaatt tccctttctt
    17281 atctggaaca actgttttcc cgtctgcgta aaaatggtct ggtttccagc gtacgtggac
    17341 caggcggtgg ttatctgtta ggcaaagatg ccagcagcat cgccgttggc gaagtgatta
    17401 gcgccgttga cgaatctgta gatgccaccc gttgtcaggg taaaggcggc tgccagggcg
    17461 gcgataaatg cctgacccac gcgctgtggc gtgatttgag cgaccgtctc accggttttc
    17521 tcaacaacat tactttaggc gaactggtta ataaccagga agtgctggat gtgtctggtc
    17581 gtcagcatac tcacgacgcg ccacgcaccc gcacacaaga cgcgatcgac gttaagttac
    17641 gcgcttaata aaaagaattc agaatcaggc cggagtgcta aatactccgt aaacacggtc
    17701 gtacatccag ccggtagcct gattccttgc attgagtgat gtacggagtt tatagagcaa
    17761 tgaaattacc gatttatctc gactactccg caaccacgcc ggtggacccg cgtgttgccg
    17821 agaaaatgat gcagttcatg acgatggacg gaacctttgg taacccggcc tctcgttctc
    17881 accgtttcgg ctggcaggct gaagaagcgg tagatatcgc ccgtaatcag attgccgatc
    17941 tggtcggcgc tgatccgcgt gaaatcgtct ttacctctgg tgcaaccgaa tctgacaacc
    18001 tggcgatcaa aggtgcagcc aacttttatc agaaaaaagg caagcacatc atcaccagca
    18061 aaaccgaaca caaagcggta ctggatactt gccgtcagct ggagcgcgaa ggtttcgaag
    18121 tcacctacct ggcaccgcag cgtaacggta ttatcgacct gaaagaactc gaagcggcga
    18181 tgcgtgacga caccatcctt gtttccatca tgcacgtaaa taacgaaatc ggcgtggtgc
    18241 aggatatcgc ggctatcggc gaaatgtgcc gtgctcgtgg cattatctat cacgttgatg
    18301 caacccagag cgtgggcaaa ctgccaatcg acctgagcca gttgaaagtt gacctgatgt
    18361 ctttctccgg tcacaaaatc tatggcccga aaggtatcgg tgcgctgtat gtgcgtcgta
    18421 aaccgcgtgt acgcatcgaa gcgcaaatgc acggcggcgg tcacgagcgc ggtatgcgtt
    18481 ccggcactct gcctgttcac cagatcgtcg gaatgggcga agcctatcgc atcgcaaaag
    18541 aagagatggc gaccgagatg gaacgtctgc gcggcctgcg taaccgtctg tggaacggca
    18601 tcaaagatat cgaagaagtt tacctgaacg gtgacctgga acacggtgcg ccgaacattc
    18661 tcaacgtcag cttcaactac gttgaaggtg agtcgctgat tatggcgctg aaagacctcg
    18721 cagtttcttc aggttccgcc tgtacgtcag caagcctcga accgtcctac gtgctgcgcg
    18781 cgctggggct gaacgacgag ctggcacata gctctatccg tttctcttta ggtcgtttta
    18841 ctactgaaga agagatcgac tacaccatcg agttagttcg taaatccatc ggtcgtctgc
    18901 gtgacctttc tccgctgtgg gaaatgtaca agcagggcgt ggatctgaac agcatcgaat
    18961 gggctcatca ttaatcggta tcggaatcag gagaatttat aatggcttac agcgaaaaag
    19021 ttatcgacca ttacgagaat ccgcgtaacg tgggttcctt tgataacaac gacgagaacg
    19081 tcggcagcgg catggtgggt gcaccggcct gtggcgacgt aatgaagttg cagattaaag
    19141 tcaacgatga aggtatcatt gaagacgcgc gttttaaaac ttacggctgc ggttccgcta
    19201 tcgcttccag ctccctggtc accgaatggg tgaaagggaa gtctctcgac gaagcgcagg
    19261 cgatcaaaaa caccgatatt gctgaagaac ttgaactgcc gccggtgaaa attcactgtt
    19321 ctattctggc agaagacgcg atcaaagccg ccattgcgga ctataaaagc aaacgtgaag
    19381 caaaataaga gttgaggttt ggttatgtcg attacactga gcgacagtgc agcagcgcga
    19441 gtaaatacct ttctggctaa ccgcggcaaa gggtttggcc tgcgtctggg cgtgagaacc
    19501 tccggatgtt caggtatggc ttatgtactg gaatttgttg acgaaccgac gccggaagac
    19561 atcgtgtttg aagacaaagg cgtgaaagtc gtggtcgatg gcaaaagcct gcaatttctg
    19621 gacggtacgc agctggactt cgtaaaagaa ggcctgaatg aagggtttaa attcaccaac
    19681 ccgaacgtta aagatgagtg tggttgcggc gaaagcttcc acgtttgatg cgcataccga
    19741 taaccccacc gtggtcgcct gcgcgtgggg tttgttttac ctgattcgcc gttatcgcgg
    19801 cggatcgcag ccctgagaat gttatggatt acttcaccct ctttggcttg cctgcccgtt
    19861 atcaactcga tacccaggca ctgagcctgc gttttcagga tctacaacgt cagtatcatc
    19921 ctgataaatt cgccagcgga agcctggcgg aacaactcgc cgccgtacag caatctgcaa
    19981 ccattaacca ggcctggcaa acgctgcgtc atccgttaat gcgcgcggaa tatttgcttt
    20041 ctttacacgg ttttgatctc gccagcgagc agcatactgt gcgcgacacc gcgttcctga
    20101 tggaacagtt ggagctgcgc gaagagctgg acgagatcga acaggcgaaa gatgaagcgc
    20161 ggctggaaag ctttatcaaa cgtgtgaaaa agatgtttga tacccgccat cagttgatgg
    20221 ttgaacagtt agacaacgag gcgtgggacg cggcggcgga tacggtgcgt aagctgcgtt
    20281 ttctcgataa actgcggagc agtgccgaac aactcgaaga aaaactgctc gatttttaat
    20341 ttctggaagc taaacatggc cttattacaa attagtgaac ctggtttgag tgctgcgccg
    20401 catcagcgtc gtctggcggc tggtattgac ctgggcacaa ccaactcgct ggtggcgaca
    20461 gtgcgcagcg gtcaggccga aacgttagcc gaccatgaag gccgtcacct gctgccatct
    20521 gttgttcact atcaacagca agggcattcg gtgggttatg acgcgcgtac taacgcagcg
    20581 cttgacaccg ccaacaccat tagttctgtt aaacgcctga tggggcgctc gctggctgat
    20641 atccagcaac gctatccgca tctgccttat caattccagg ccagcgaaaa cggcctgccg
    20701 atgattgaaa cggcggcggg gctactgaac ccggtgcgcg tttctgcgga catcctcaaa
    20761 gcactggcgg cgcgggcaac tgaagccctg gcaggcgagc tggatggtgt agttatcacc
    20821 gttccggcgt actttgacga tgcccagcgt cagggcacca aagacgcggc gcgtctggcg
    20881 gggttgcatg ttctgcgctt acttaacgaa ccgaccgctg cggctatcgc ctacgggctg
    20941 gattccggtc aggaaggcgt gatcgctgtt tatgacctcg gtggcgggac gtttgatatt
    21001 tccattctgc gcttaagtcg cggcgtgttt gaagtgctgg caaccggcgg tgattccgcg
    21061 ctcggcggcg atgatttcga ccatctgctg gcggattaca ttcgcgagca ggcggacatt
    21121 cctgatcgta gcgataaccg cgttcagcgt gaactgctgg atgccgccat tgcagccaaa
    21181 atcgcgctga gcgatgcgga ctccgtgacc gttaacgttg cgggctggca gggcgaaatc
    21241 agccgtgaac aattcaatga actgatcgcg ccactggtaa aacgaacctt actggcttgt
    21301 cgtcgcgcgc tgaaagacgc gggtgtagaa gctgacgaag tgctggaagt ggtgatggtg
    21361 ggcggttcta ctcgcgtgcc gctggtgcgt gaacgggtag gcgaattttt cggtcgtcca
    21421 ccgctgactt ccatcgaccc ggataaagtc gtcgctattg gcgcggcgat tcaggcggat
    21481 attctggtgg gtaacaagcc agacagcgaa atgctgctgc ttgatgtgat cccactgtcg
    21541 ctgggcctcg aaacgatggg cggcctggtg gagaaagtga ttccgcgtaa taccactatt
    21601 ccggtggccc gcgctcagga tttcaccacc tttaaagatg gtcagacggc gatgtctatc
    21661 catgtaatgc agggtgagcg cgaactggtg caggactgcc gctcactggc gcgttttgcg
    21721 ctgcgtggta ttccggcgct accggctggc ggtgcgcata ttcgcgtgac gttccaggtc
    21781 gatgccgacg gtcttttgag cgtgacggcg atggagaaat ccaccggcgt tgaggcgtct
    21841 attcaggtca aaccgtctta cggtctgacc gatagcgaaa tcgcttcgat gatcaaagac
    21901 tcaatgagct atgccgagca ggacgtaaaa gcccgtatgc tggcagaaca aaaagtagaa
    21961 gcggcgcgtg tgctggaaag tctgcacggc gcgctggctg ctgatgccgc gctgttaagc
    22021 gccgcagagc gtcaggtcat tgacaatgct gccgctcacc tgagtgaagt ggcgcagggc
    22081 gatgatgttg acgccatcga acaagcgatt aaaaacgtag acaaacaaac ccaggatttc
    22141 gccgctcgcc gcatggacca gtcggttcgt cgtgcgctga aaggccattc cgtggacgag
    22201 gtttaatatg ccaaagattg ttattttgcc tcatcaggac ctctgccctg atggcgctgt
    22261 tctggaagct aatagtggtg aaaccattct cgacgcagcg ctgcgtaacg gtatcgagat
    22321 tgaacacgcc tgtgaaaaat cctgtgcttg caccacctgc cactgcatcg ttcgtgaagg
    22381 ttttgactca ctgccggaaa gctcagagca ggaagacgac atgctggaca aagcctgggg
    22441 actggagccg gaaagccgtt taagctgcca ggcgcgcgtc accgacgaag atttagtggt
    22501 cgaaatcccg cgttacacta tcaaccatgc gcgtgagcat taacagaggt tagtatggga
    22561 cttaagtgga ccgatagccg cgaaattggc gaagcactgt acgatgcgta tcccgatctt
    22621 gatccgaaaa cggttcgatt caccgatatg catcagtgga tttgcgatct ggaagatttc
    22681 gacgacgacc cgcaggcatc caacgagaaa atcctcgaag cgattttgtt agtctggctg
    22741 gacgaagccg agtgaatgaa cgggctgcct gggggtagcc cgttttgctg ttaaacgaat
    22801 aaggataact aaaatgacag aagcgatgaa gattaccctc tctacccaac ctgccgacgc
    22861 gcgctgggga gaaaaagcaa cttacagcat taataatgac ggcattaccc tgcatttgaa
    22921 cggggcagac gatctggggc tgatccagcg tgcggcccgc aagattgacg gtctgggcat
    22981 caagcatgtt cagttaagcg gtgaaggctg ggatgcggat cgctgctggg cattctggca
    23041 aggttataaa ggcccgaaag gcacgcgtaa agtggagtgg ccggatctgg acgatgccca
    23101 gcgccaggaa ctggataacc gcctgatgat catcgactgg gtgcgtgaca ccatcaacgc
    23161 accggcagaa gaattgggac catcgcaact ggcacagcgt gctgttgatc tgatcagcaa
    23221 cgtcgcgggc gatcgtgtga cttatcggat caccaaaggc gaagatctgc gtgagcaagg
    23281 ttatatgggg ctgcacactg tcgggcgcgg ttcagaacgt tctccggtat tgctggcgct
    23341 ggattacaac ccaactggcg ataaagaagc accggtgtac gcgtgcctgg taggtaaagg
    23401 tatcactttt gactccggcg gctacagcat caaacagact gcgtttatgg actcgatgaa
    23461 gtcggacatg ggcggcgcgg caacggttac cggggcgctg gcatttgcca ttacgcgcgg
    23521 actgaacaag cgcgtgaagc tgttcctctg ctgtgcggat aacctgatta gcggcaatgc
    23581 gttcaagctg ggcgatatca tcacttatcg caacggtaaa agagttgaag tgatgaacac
    23641 tgatgccgaa gggcgtctgg tgcttgccga tggtctgatt gatgccagtg cgcagaaacc
    23701 ggaaatgatc attgatgcgg cgaccctcac cggggcggcg aaaactgcgc tgggtaatga
    23761 ttatcacgcg ctgttcagtt ttgacgatgc gcttgccggt cgtttactgg cgagtgcctc
    23821 acaagagaac gaaccattct ggcgtctgcc gctggcggaa ttccaccgca gccagctgcc
    23881 gtctaacttt gccgaactga acaataccgg aagcgcggcg tatccggcag gcgcgagcac
    23941 ggcggcgggc ttcctgtcgc actttgttga gaactatcag caaggctggc tgcatatcga
    24001 ctgctcggcg acttaccgta aagcgccggt tgaacagtgg tctgcgggcg ctacgggact
    24061 tggtgtgcgc acgatagcta atctgttaac ggcgtaaact aatatgccgg atgcggcgta
    24121 aacgccttat ccggcctaca aattctgcaa attcaacaaa ttgcgaatcc cttgtaggcc
    24181 tgataagcgt agcgcatcag gcatttgcgc cccgaacgtt gaatgaattg tggttcttat
    24241 gtccgaaaca aaaaacgaac ttgaagacct gctggaaaaa gcagcaaccg aaccggcgca
    24301 ccgcccggcc tttttccgta ctctactgga atccaccgtc tgggtgcctg gtacggcggc
    24361 gcagggcgag gctgtggttg aagattgtgc gcttgattta cagcactggg aaaaagaaga
    24421 cggcaccagc gtcattcctt ttttcacctc gttagaagcg cttcaacagg cggttgaaga
    24481 cgaacaggca tttgtcgtaa tgcccgttcg cacattgttt gagatgaccc ttggcgaaac
    24541 gctcttcctt aatgccaaac tgccgaccgg taaagaattt atgccgcgtg aaatcagttt
    24601 gttgattggt gaagagggaa atccgctgag cagccaggaa atcctggaag gcggtgaatc
    24661 gctgatatta tcggaagtcg cagagccgcc agcacaaatg attgattcac tcaccacctt
    24721 atttaaaacc attaagccgg tgaagcgtgc ttttatttgt tcaattaaag agaacgaaga
    24781 ggcacagcct aatttactta ttggcattga agccgatggt gatatcgaag aaattattca
    24841 ggcgacggga agtgtagcga ccgatacatt acctggcgat gaaccaatcg atatttgtca
    24901 ggtgaaaaaa ggggaaaaag gaattagcca ctttattacc gaacatattg cgccattcta
    24961 tgaacgtcgc tggggtggtt ttttgcgtga ctttaaacaa aaccggataa tctaaaatat
    25021 gccgccctaa agggcggcat tcgggcttac ttgctgctct gaaggttgtt aaccaccagt
    25081 aaattgtcgc cggagagact ccggttatta tttacttggg aaaacagcgc accagaaaca
    25141 ataagtacga tacagaagca ccgtatcttc attgtgctgt cgtgatgcga agaccgcatc
    25201 acgacagcac cactggcgat attgccgcga tagcaagcgc tgagacgcgc ttgctctaac
    25261 ggtcgcggaa catcctttcc ttaagccagc gcccgcttgc gagacggcgg aaagggccgc
    25321 agtggataat acactaacag aacaataata actctctttt gcttgacaaa aagagagtta
    25381 ctggcgagta ttgtctccgc tgagacgcgc ttgcattact acggtaatac aacctgctgg
    25441 aaaggccgcg aaccagacca gcaataaaaa aacgccaaat tcggcggttt tttattgcct
    25501 gtaaaatact tatttcactg gctcaaccgg taaatctgcc cgcgcgcccc attcactcca
    25561 tgcgccgtcg tacagtttca cgtttggcac atccagcgtc gcgagtgcta acaaaaccac
    25621 ggctgccgtt acaccagagc cgcagctgac gataatcggt ttgtcatagc tgacgccgcg
    25681 accaaaaaat atcgcatcca gttcatcggt cgtttttagt tcgccttcgc gcaccagttc
    25741 cgtccacgga acgttcaatg ctccgggaat atgtccgcga cgtaaacctg ggcgaggttc
    25801 atcaacttct gcgttaaaac gtgcagccgg gcgggcatca ataatttgcg ccgtattttc
    25861 atggcttgcc aataacacat cggttacttt caccactgct tcaggattaa acgcggcgtt
    25921 aaactctcct tccggcagct ctactgcacc ttcttctaat agcaggtcat cgcgctgcca
    25981 gcctgcaag
//
LOCUS       DADMGC010000049        25908 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.049, whole genome shotgun sequence.
ACCESSION   DADMGC010000049 DADMGC010000000
VERSION     DADMGC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.049"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..1308)
                     /locus_tag="JNZ73_001986"
     CDS             complement(<1..1308)
                     /locus_tag="JNZ73_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAY8732282.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRPLILQFNMCTPGREDAEVKLYSKAEYKASRG"
     gene            1603..2277
                     /locus_tag="JNZ73_001987"
     CDS             1603..2277
                     /locus_tag="JNZ73_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY8732283.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNGLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(2316..3977)
                     /locus_tag="JNZ73_001988"
     CDS             complement(2316..3977)
                     /locus_tag="JNZ73_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HAY8732284.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(4173..4601)
                     /gene="ibpB"
                     /locus_tag="JNZ73_001989"
     CDS             complement(4173..4601)
                     /gene="ibpB"
                     /locus_tag="JNZ73_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HAY8732285.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(4713..5126)
                     /gene="ibpA"
                     /locus_tag="JNZ73_001990"
     CDS             complement(4713..5126)
                     /gene="ibpA"
                     /locus_tag="JNZ73_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HAY8732286.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            5431..5763
                     /locus_tag="JNZ73_001991"
     CDS             5431..5763
                     /locus_tag="JNZ73_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAY8732287.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGSKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(5765..7015)
                     /locus_tag="JNZ73_001992"
     CDS             complement(5765..7015)
                     /locus_tag="JNZ73_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HAY8732288.1"
                     /translation="MMAGTVLYQDRAMKQITFAPRNHLLTNTNTWTPDSQWLVFDVRP
                     SGASFTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWH
                     YDFHHRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVM
                     HQLDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAY
                     EEGWVGNHALAFIGNTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPP
                     RGVVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGE
                     PRKLTHNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAV
                     VFSPDGQWLAWMEGGQLWITETDR"
     gene            7080..8098
                     /locus_tag="JNZ73_001993"
                     /pseudo
     CDS             7080..8098
                     /locus_tag="JNZ73_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(8059..9396)
                     /locus_tag="JNZ73_001994"
     CDS             complement(8059..9396)
                     /locus_tag="JNZ73_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8732289.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(9471..10619)
                     /gene="dgoD"
                     /locus_tag="JNZ73_001995"
                     /pseudo
     CDS             complement(9471..10619)
                     /gene="dgoD"
                     /locus_tag="JNZ73_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(10616..11233)
                     /locus_tag="JNZ73_001996"
     CDS             complement(10616..11233)
                     /locus_tag="JNZ73_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HAY8732290.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKVVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(11217..12095)
                     /locus_tag="JNZ73_001997"
     CDS             complement(11217..12095)
                     /locus_tag="JNZ73_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HAY8732291.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLLR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(12092..12781)
                     /gene="dgoR"
                     /locus_tag="JNZ73_001998"
     CDS             complement(12092..12781)
                     /gene="dgoR"
                     /locus_tag="JNZ73_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HAY8732292.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            13059..13715
                     /locus_tag="JNZ73_001999"
     CDS             13059..13715
                     /locus_tag="JNZ73_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732293.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(13761..14573)
                     /gene="yidA"
                     /locus_tag="JNZ73_002000"
     CDS             complement(13761..14573)
                     /gene="yidA"
                     /locus_tag="JNZ73_002000"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAY8732294.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEMANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(14687..15094)
                     /locus_tag="JNZ73_002001"
     CDS             complement(14687..15094)
                     /locus_tag="JNZ73_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HAY8732295.1"
                     /translation="MIIMGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(15325..17739)
                     /gene="gyrB"
                     /locus_tag="JNZ73_002002"
     CDS             complement(15325..17739)
                     /gene="gyrB"
                     /locus_tag="JNZ73_002002"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAY8732296.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(17768..18841)
                     /gene="recF"
                     /locus_tag="JNZ73_002003"
     CDS             complement(17768..18841)
                     /gene="recF"
                     /locus_tag="JNZ73_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAY8732297.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPRFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(18841..19941)
                     /gene="dnaN"
                     /locus_tag="JNZ73_002004"
     CDS             complement(18841..19941)
                     /gene="dnaN"
                     /locus_tag="JNZ73_002004"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAY8732298.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(19946..21349)
                     /gene="dnaA"
                     /locus_tag="JNZ73_002005"
     CDS             complement(19946..21349)
                     /gene="dnaA"
                     /locus_tag="JNZ73_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAY8732299.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLSLYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            21646..21726
                     /locus_tag="JNZ73_002006"
     CDS             21646..21726
                     /locus_tag="JNZ73_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732300.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            21956..22096
                     /gene="rpmH"
                     /locus_tag="JNZ73_002007"
     CDS             21956..22096
                     /gene="rpmH"
                     /locus_tag="JNZ73_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAY8732301.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            22113..22472
                     /gene="rnpA"
                     /locus_tag="JNZ73_002008"
     CDS             22113..22472
                     /gene="rnpA"
                     /locus_tag="JNZ73_002008"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAY8732302.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCLLARGS"
     gene            22436..22693
                     /gene="yidD"
                     /locus_tag="JNZ73_002009"
     CDS             22436..22693
                     /gene="yidD"
                     /locus_tag="JNZ73_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAY8732303.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            22696..24342
                     /gene="yidC"
                     /locus_tag="JNZ73_002010"
     CDS             22696..24342
                     /gene="yidC"
                     /locus_tag="JNZ73_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAY8732304.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            24448..25812
                     /gene="mnmE"
                     /locus_tag="JNZ73_002011"
     CDS             24448..25812
                     /gene="mnmE"
                     /locus_tag="JNZ73_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAY8732305.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6281 a         6992 c         6608 g         6027 t
ORIGIN      
        1 gcctcgcgag gctttgtatt cggcttttga gtagagtttc acttccgcat cttcacgtcc
       61 cggcgtgcac atgttgaact gcagaatcag ggggcggaaa accacgaagt acagcagggt
      121 aaagcacaac ccaatggcga tttgggtcag catcatatcc gcgtggttgc tgaacatcgg
      181 gatccagttt tgcggtaaaa cctggtcaat cagacctccg cccatgttgc ccaccacacc
      241 aaagagatac attacggtcg acattgaggc cgccagcacg gcgtgtaccg caaacagcaa
      301 cggtgaaatg aacaggaagg taaattccag cggttcggta attcccacca gcatggcggt
      361 taaggttgcg ggaatcagca agcccgccac ttttacccga ttttccggtg cggcagtgaa
      421 gtacatcgct aaagaaatgc ccacggcacc aaagattttt gagttaccgt gcagggcaaa
      481 accgccttcc gggaacaacg atttcagcgg ctcggcgctc agactgaact cttgcagatg
      541 ctgcgcccag tacatctgaa tgccgccttc aacagctgcc ggaccaaaga taaacggtcc
      601 gtagatgaag tggtgtaaac cggttgggat cagaatacgt tcgaggaagg tgtaaaccca
      661 caccccaagc gcacccgccg aacgcaggaa cgcttgcaga gattcaatcc ccatttgtac
      721 ttttggccag ccgagcaatg tcagccaggc acaggggatc atcaccagga aggcgataat
      781 caccacataa gacgtcccct ggaaaatgcc gagaaaaacc ggcagttttt tatcgaacag
      841 acggttatgc agcgccgtca caatgccgga aatgataatt gcgccgataa tgctggtatc
      901 gagggtttta atcccggcca tcattgtcag accgctacct gccaccgcgt cctgagtgaa
      961 atcgacgccg aagtagcttc cccaggtcat tcccatcgca ttgatgaaat agttccaggt
     1021 caggaaactc accatcaccg ccagacaagc acgtccctgc gcttgcttag caaggccaat
     1081 gggtaaaccg acagcaaaaa tcagcggcat attacggaat accgtccaac cgccctcttc
     1141 aataatgtgt acgatttgcg cgaataaact gttcggatcg gtcagtgatt ccccgacaaa
     1201 catcgggttt tgcagcaaga tggcaagacc caccacaatc ccggcgaagg gaaacagcag
     1261 cactggcgtg aacatcgcgc cgccaaagcg ttgaatttga ctgagcattt ccacatcctc
     1321 ttttctcaat tctgtaagta ggaagcctga ttggttatgc ggcagacctg gagcgaggat
     1381 aggaagatgc gatcggcaga acatgacgac ccaatgggat tcgtgatcgc tttcatgctt
     1441 ttcgttgggc ggtagagatc tacgttttcg cgaaaaagta gacatctgtg agcacaggta
     1501 cacagcatcg aacacatctt taaaaaaaag atttttttca atcgattaag cagaacttgt
     1561 gggcgcatta cccgtgcttg caggcaaaaa agagagctag agatgatcta caaaagcatt
     1621 gcggagcggt taagaattcg acttaattcc gcggatttca cgctaaacgg ccttcttccc
     1681 ggtgaaaaaa agctggcgga agagtttgcg gtatcgcgga tgactatccg taaagccatt
     1741 gacctgctgg tagcgtgggg gctggtggtc cgccgccacg gcagcggcac ttacctggtg
     1801 cgcaaagatg tgctgcatca aaccgccagc ctgaccggac tggtggaggt gttaaaacgg
     1861 cagggaaaaa cggtcaccag ccaggtgctg atttttgaaa tcatgcctgc gcctccggcc
     1921 attgccagcc agttacggat tcaaatcaac gagcagatct acttctcccg tcgcgttcgt
     1981 tttgtggaag ggaaaccgct aatgctggaa gacagctata tgccggtaaa actgttccgt
     2041 aacctttcac tgcaacatct ggaagggtcg aagtttgaat atattgaaca agagtgcggg
     2101 attttgattg gcggtaatta tgaaagcctg atgccggtgc tcgccgatag actgctggcg
     2161 cggcaaatga aggtagcgga acacacgcca ctgctgcgga tcacctcgtt gtcatatagc
     2221 gagagcgggg agtttttgaa ttattcagtg atgttcagaa atgccagcga atactaggtg
     2281 gagtaccatt tacggcgact ccacccggaa aagagttaac cgatactcca gaagagcacc
     2341 gccagtaatt ggggggtgat aatgcgcagg aacatcacta acggatagac agtggcgtaa
     2401 gagagcgccg ccgcaccact ggttggatga agattattag caaacgccag cgccggagga
     2461 tcggtcatgg aacctgccag catcccgcac atggtcaggt aattcatttt ggctaacatc
     2521 cgcgccagaa tgccaacagt aatcagcgga acggcggtga tcagggcacc ataaccaatc
     2581 cagcttagcc cttcgccatt gaccagggta ttcacaaaat ccccaccaga tttcagacca
     2641 acgaccgaga ggaacagcac gatccccagc tcccgcagcg cgaggttggc acttggcggc
     2701 ataaaccagt acagcttgcc gatactgccg atacgcccga ggatcaacgc cataatcagc
     2761 ggaccgcccg ccagccccag tttcaacgcg gccgggaatc ctggcacaaa gacgggaata
     2821 gaacctaaca atacgccaag cccgatgcca ataaacactg gcagcatctg aacctgttgc
     2881 agtttttgct gcgcattccc cagcacattg gcaacggcat caattgcgga cggacgcccc
     2941 accaggttga ggatatcgcc gaactgcagg ctgatatcgc cactggcgac cagttcgacc
     3001 ccggcacggt tcaggcgcga gataacaacg tcatagcgtt ctttaaagtg caggtcgcga
     3061 atacgttttc cgagcacgtt ttcattggtg accaccacac gctcgacgcg caaatcagtg
     3121 cctttcgtag acagcgaggt atcgacctcc tgaccaatca ccagttgcgc attatgtaaa
     3181 tccgctggct gccccaccag atgcagcaaa tcgcccagtt ggataatggt atctggcgaa
     3241 ggtactttta gggtttcttc tcgtttcaga cgcgagcaga taattttgtc gccgttgaga
     3301 atcggtacat ctttaatggc taaatcatgc aggttagggt tctcaacgcg aatattgata
     3361 gtcttgatca gcgcgccgcc attggtgcgt gaagactcgt gctgctgagc ttctgtctcg
     3421 acattgacgc ggaaaataac ccgcaacatc cacatggtga acaaaatccc gcaaatgccg
     3481 aatggatacg ccatcgcgta actcatcccc atctgatcga ccatttccat tggtgtaccc
     3541 aggtcgcgca agatctgctg ccctgccccc agcgctggcg tattggttac cgcaccggag
     3601 aaaatcccca gcactaccgg cagtggaata tcaaacagtt tatgcaggat ggcggtaacc
     3661 agaccaccga tgatgacgat cagaacagca aacaggttga ggcgtaatcc ggagacgcgc
     3721 aatgaggcaa agaagcccgg ccctacctga atcccgatgg tataaacgaa caggatcagg
     3781 ccaaattcct gaataacatg cagcatatcg ctacttaatg tcatccccgc ctgagaaaca
     3841 aaatggccga cgatgatccc accaaacagc acgccgccaa tacctaatcc tatgccgcga
     3901 aatttgacgt tgccgataaa caaaccgacg actgccacca aagccagaat actgaccgtt
     3961 aatgctatat cactcatcgt ccatttctct tgagataaca ttttaattgc tacggattat
     4021 ggctgaatcg gtgaagcaga tttgccttat agcgcacatt atcacgggaa agaacgtgcc
     4081 gaaatatctt aaacagtcga aatttaacgc attgttgaag aagaaagaca aaaaaagccc
     4141 caccagaatg gcggggcaaa gagaatagct agttagctat ttaacgcggg acgttcgctg
     4201 atagcgatac gctgcgctgc gatgggttct ggctcattac gaattaaatc aatatgcagt
     4261 aaaccgttta cgaaggttgc gccagagact tccatatttt cagccagcgt aaaactcagg
     4321 ctaaatggct gattcataag cccttgatgc agccattttt tctcttcttt tggctgctcc
     4381 ggcgtgcctt ttacgctcag gcgcgtacct tccagttgaa tctctaaatc ttcctgacgg
     4441 aaacctgcca gcgcaagggt aatgcggtag tggttatcgt cgcttttctc aatgttgtac
     4501 ggcgggaagc tctggctttc accggcgttt tgcagtgcgt tggccagttt gtcaaaaccg
     4561 atccattgac gcatcagtgg ggataaatcg aagttacgca tagtcatttc tccttctaag
     4621 aagcgagtaa gtacctgcaa atccgaagat tcgcatatgc tccctgacgg cgagcatgga
     4681 gatgtcaggc cgcgccaggc ggccttaggg aattagttga tttcgatacg gcgcggtttt
     4741 ttcgcttccg gaatcacgcg ttcgagatcg atatacagca aaccatttac caggttagcc
     4801 ccacgaacat gaatgttctc agctaactgg aatttgcgtt caaagttgcg ttcagcgatg
     4861 ccctggtaca gataggtgcg ctctttttgt tcgtcggcgt gagcaccttt caccaccagc
     4921 agattatcct gggcggtaat ttccagttcg ctctcagcaa aaccagccac agcgatagca
     4981 atgcggtaat ggttttcgtc taccagttca acgttatacg gagggtagcc gccattactc
     5041 tggctctggt tgttttctaa gtggttaaac aaacggtcaa atccaatagc agaacggtaa
     5101 agcggggata aatcaaagtt acgcataatc aatagctcct gaaatcagcg agaatgtaag
     5161 accttccaca atggacaggt caggtagcca gaacacccat caggcgcgtc ctcatcgact
     5221 acgatgtaaa atgggtctgg aaatgaactt ttcaagcctt atcagcggac ttttttttgc
     5281 agtttatggt ctattgcata gactgagggg gcagcacgaa acgttaaagt gcgatagccg
     5341 ccacagtgcg acgtaatgat gatgttattt tcagcaaggg atcgctataa ctcatcaggc
     5401 caacacccac gatcaacaga tgaatttatt atgataagaa atgtgttgtt agcgttcatg
     5461 atatgcagcg gaatgacatt actcggagga tgctccagcg tgatgtccca taccgggggg
     5521 aaagaaggaa cgtatcccgg cacccgcgcc agcgccacca tgattggcga cgatgaaacc
     5581 aactggggct ctaagtcgct ggcgattctc gatatgccgt ttaccgctgt gatggacacg
     5641 cttttactgc cgtgggatgt gttccgcaaa gacagctcgg tgcgctcgcg cgttgaaaaa
     5701 agcgaagcca acgcgcaagc caccaatgcg gttattccac cggccagaat gcctgataac
     5761 tgatttagcg atcggtttcg gtgatccaca gctggccacc ttccatccac gccagccatt
     5821 gaccatccgg cgagaagacc acggcgtccg cagaaggtgg attggcgtgg ttttcggtta
     5881 aatactcaac ctcgccgctt tgcgcatggg cacaaacaat tcgattatcc agcacaaagc
     5941 ccaaccattc tcctgacgga tgccagttaa atgcagactg aatatccgtt ttgttatggg
     6001 ttaacttgcg cggttcgccg ccctgtggcg agataagcca cagttgcaca atgccgttat
     6061 catcacgcat taaaaacgcg atttgcgtac cctgcggatt acagcgcacc cagtggcgcg
     6121 ggacgttgac taaccccgga taagcccgat gatgggtaaa ggttaaacgt cgctgcacga
     6181 cgccacgcgg tggcgcgggt agggttgttt ccgttccact taacggcgca tcacctgccg
     6241 atttccagct agcttcatct tgcggtaact caacgataaa tagctccggc actttctcgc
     6301 cctttggcga aagtgtgttg ccaataaacg ccagcgcgtg atttcctacc catccttctt
     6361 cataagcacg attgatttca tcactgccag gctgcggcgt gggcgtggtt ttactcacca
     6421 gcacacacca gtggctaccg ctgtattcac gcggatgttg cttttgtacg ttgaccgggc
     6481 caaacggtgc agcaaccccg acgtttcgca aatccagcgc cggatcgagt tgatgcatta
     6541 catggtcgtt ataggtaaag ctcccccttt caccgttcgg gctaaagaca tgcacatggc
     6601 tgccaccgcg cagcgctcct ggcgtatacg gcgcggtaat atccattgca tcgagattgc
     6661 tcaccttgcc gccttcagca atgactccgc gtcgatgatg gaagtcgtaa tgccatgttt
     6721 catcaggatt ttcagggccg tgaataaaga catatttctc tgacttcgga tgaacggtca
     6781 ccacgccgac gtgtgcgccc tgtgacgcgc gatagataac ctcgacctcg cctgtatgga
     6841 tattcacacg ctcaatggtc tcgccggtaa acgacgcccc agaaggacgc acgtcaaata
     6901 ccagccactg gctgtcgggc gtccaggtat tggtattggt gagcaggtga ttacggggag
     6961 caaaggttat ctgtttcatg gcgcggtcct gatacaaaac ggttcccgcc atcatacttc
     7021 acaaggactt cactttcaga caatagtctt ttgccatcgt gatgaatgtg agtgaaaaga
     7081 tggaacattt cgacgtggcg attattggcc tcggcccggc agggtcggcg ttggcacgaa
     7141 agttagccgg caaaatgcag gtgatcgcgc tggataaaaa gcaccagtgc ggtactgaag
     7201 gtttcagcaa accttgcaga ttttcagcgt caaaactgtc gacgtcgccg catcgctcac
     7261 gcgtaactac cagcgaagct atatcaatat taatcgccat gctttcgact tgtggatgaa
     7321 atcgctgatc cccgccagcg ttgaggttta tcacgatagc ctgtgccgaa aaatctggcg
     7381 tgaggatgat aaatggcatg tcatttttcg tgcagacggc tgggagcaac atattaccgc
     7441 ccgctatctg gtcggtgccg atggcgcaaa ctcgatggtg cggcgacatc tctacccgga
     7501 tcatcaaatc cgtaaatatg tcgctatcca gcagtggttc gcagagaaac atccggtgcc
     7561 gttctactcc tgcatctttg ataatgcgat aactgactgt tactcatgga gtatcagcaa
     7621 agatggttat tttatctttg gcggtgccta tccaatgaaa gacggtcaga cgcgtttcac
     7681 gacgctgaaa gagaaaatga gcgcctttca gttccagttt ggcaaagcgg tgaaaagtga
     7741 gaaatgcacg gtgttgtttc cctcacgctg gcaggatttt gtctgtggta aggacaacgc
     7801 ctttctgatt ggtgaagcgg cgggatttat cagcgccagc tcgctggagg ggattagcta
     7861 tgcgctggat agcgcagaga ttctgcgtgc ggtgttcctg aaacagccgg agaagagcaa
     7921 cgccgcttac tggcgcgcca cacgcaaact gcgtttaaaa ctcttcggca agatagtaaa
     7981 aagccgatgc ctgaccgcac cggctttaag aaagtggatt atgcgcagtg gtgtggcgca
     8041 tattccacag ttgaaagatt atccaacgcg cttcacatca cccaccagca ggatgtaaga
     8101 gagcgcgcca atcaacgcga cggcggagat ataaaccagt gcaggcgcga aaccgtaacc
     8161 ctgcgccagg taccccacca ccagcggaac ggtgatgccg cccagaccac cggcgaagtt
     8221 aaacacgccg ccggttaaac caatcaggcg catcggtgcc agagaagaga ccagcgacca
     8281 ggtaatcgaa gcaaaaccgt tgccgaagaa tgccagcgcc atcaggcaca taatcatcat
     8341 cggatcgttg gtgtagttag cgcccataat gcaggtggag atcagcaagc cgcagataat
     8401 cggcgtttta cgcgcaaagc ccagtgaaaa gcccttacgt accagcagat ccgcgaccca
     8461 gccagagagc aggacgccga caaacgccgc gaggaatggc accgtggtca taaagcctgc
     8521 tttcagcgcc gtgattcctt tttcctgggt taaatagttc gggaaccagg ttaagaaaaa
     8581 ccacagtgca gaagccaccg caaattgccc aagataaacg ccgatcagtt tacgatggaa
     8641 caccagtttc cagtctttgg ctgttaacgg ctgacgcgcc tctttcttca ccggcgcatc
     8701 accatccacc agaccgccgc catcacgaat gtaatccagt tcagctttgc tgataccttt
     8761 ggtcaggcgc ggcggctgat aaaccttaaa ccaaatcagc gaccagataa tgccgatacc
     8821 accagtgaca atgaacaccc agtgccagct caacatctcc tgaatccaga tcagcagcgg
     8881 agtcaaaaac gccagaccga caaactgacc agacgtataa aaaccaacgg cggaagcgcg
     8941 ttcatgttcc ggaaaccagc tggtcaccat ccggttattg gtcgggaaag cgggcgcttc
     9001 gaaaataccg gttatcgcac gcagaccaat taatgacatt aatcccgtgg caaagccctg
     9061 gaataaagtc gccactgacc aaccaaatat cgcaataaaa taagtcacgc gagaacctac
     9121 gcgatctaaa aaccaaccgc cggggatctg gcatagcgta taaagccagg cgaaggccga
     9181 aaatacatag cccatttccg ctttcgtaat gccgaactct tcctgaatat gggcggaagc
     9241 cacggccagg ttagcgcggt caacataaca aatgaccacc gtaataaaga tcatcaccag
     9301 cgtcagataa cgccgacgcc ccggctttgc tgcattaacg ggaatatcca tagcgagctt
     9361 tctccagatt ttgggcatag cgaagccgct caccatgccc tgtaatttac agagggttat
     9421 atttttgtat tgctgtttta gtgcccgatg aggggcttac gtggcaggaa ttaccactct
     9481 gctacgctgt tatcttcatg acgccagagc ggattacgcc agtccggggc atttttactg
     9541 aactcaataa ctttagcttc gtcgatttcc acacctaagc ccggtttcgt taacggttta
     9601 aagaagccgc caaccatgct gaagtcttct ttgtttttca caaagtcgag taactccgcg
     9661 cctttgttgt aatgaatgcc catactttgt tactgaagta ccgcgttata ggaaacaaag
     9721 tcgatatgca ggcaagccgc cagtgcaatc ggtccgaacg gacagtgcgg cgcaagggtc
     9781 acatcatagg cttctgccat tccagcaatt ttgtagcatt cggtaatacc gcctgcatgg
     9841 gagagatccg gttgcagaat cgaaataccg cctgcctcca gcacgcgttt aaaatcgaaa
     9901 cgtgagaaca tgcgctcacc cgccgccagt ggaatatgcg tttgtgccgc caatttcggg
     9961 tagtattcgg cttgttccgc cagcaccggc tcttcaataa acagcgggcg atacggctcc
    10021 agttctttaa tcagcacttt cgccattggc gcgctgacgc gaccgtggaa atcaagacca
    10081 aactcaatct gattgccaaa agcttcacga atttgtgcca cggtgttgac tgccgcatct
    10141 accgcgcggg agttatcaat tagccccagt tcttcacaac cgttcagttt gaaggtatcg
    10201 aagccgattt cgcgcagggt tttaatgccg tcgataacat ccgccggacg atcgccgccg
    10261 acccaactgt aggctttaat tttgtcgcga accaggccgc ccatcagttg ccagactggc
    10321 gcattcagca ctttgccttt gatatcccat aacgcctggt cgatcccggc gatggcgctc
    10381 atcaggattg gaccgccacg ataaaatccg gcgcgataca tcacttgcca taagtcgttg
    10441 atgcgcgaag gatcctgacc aatcaaatag tcacccagct cgtgaactgc cgcttccacc
    10501 gtacgggcgc ggccttcaat cacgggctcg ccccaaccga ccacgccttc atcggtttca
    10561 attttcagga acatccagcg gggaggtaaa cgatacgtgg taattttggt gattttcatt
    10621 gcactgcctc tcgatacgcc ttaacaaatg ctgctgcctg ctgcgcggtg cgttctacgg
    10681 actgcccggc gcgataaaga tcgctgccta agcccgcccc aacacaacct gcgtctatcc
    10741 actgcgccag gttttctggc gtcacgccgc caacggcaaa gactgcgatg tccggtggta
    10801 ataccacttt taacgctttg atgtattgcg gaccaaaagc cgatgacgga aatattttca
    10861 gcgcctgcgc gcccgcttcg agcgcggtaa aggcttcggt cgccgtcgcg cagccggggc
    10921 aaacggtcat gccgtagccc accgcgcggc ggatcacttc actatggata ttgggcgtaa
    10981 cgatgagctg acagcccatc ctggcgagcg catcgacctg ttcaggtttc agtaccgtac
    11041 ctgcgccaat caacgccttg tcgccatacg catcaacgat ggcgggaatg ctctgctccc
    11101 attgtgggga attcagcggg atttcaaccg cgtcgaaccc ggcgtcaatc accgcgccaa
    11161 catgcgccag cgcctcgtcg ggcgtaatgc cgcgcaaaat ggcgatcagc gggagtttag
    11221 tttgccactg catgagcgat gctccttata ccagcctgaa atgccgtgtc gcccgccacc
    11281 gccgccacgt cgcaacccat cgcctgaaag gcttgctggt agcgcgcggt cagcgatgtt
    11341 ccggcgacaa gggtgatggc gtgttgatgg gtcacatagt cgcgcatact ggcaacctct
    11401 gcgccaatca acaaaccaga gagaaattcg ctgacctgtt cgcggagaag tgttcccagc
    11461 acatgcgagg cgcgaacttc aaaaagctgc ggcaatatgg cgggcgcatt aaggccgcgc
    11521 tcaaggccag ccgcgaaggc atcggcagag ttttcctgcg gcggcaaacc tgcgccaatc
    11581 aatgagtgat ttaacagtaa atgatgtaat tcaccggtca tcacggtgcg aaaatcgttg
    11641 atttgctggc tatcggcctg cacccatttg caatgcgtac cgggcataac ataaagagag
    11701 gaaggagcca gagctcgcgc gccgatcaat tgtgtttctt cgccgcgcat cacattgtgg
    11761 ttatcgtcat gagagacgca taatccggga ataatccaga tattgtcgcc aacagacgtt
    11821 aattgttcgc caatagacga aaaacgggca ggaacagata aatacggagc aactttccag
    11881 ccgacattgc tgccgaccat tcctgccatc accactggcg tattttcttc acgccagtcg
    11941 gtcgtgactt ctgctaacac cgcagccgga gattttccgt tcaggcgcgt gacgcctgct
    12001 tctgattgcc tgctctccag gcagtggtcg ccctgataaa gccaggcgcg cagattggtc
    12061 gatccccagt caattgcgat gtagcgagct gtcatgtgat ttcctttaac cttcgtgtcg
    12121 agctggcaat catggtaagc gccgcctgct ctgccgcatc gccgtcctga tgccgtatcg
    12181 catcgaacag cgccttatgt tcctggagcg tttgcggcat gttggcctca tcgcccatcc
    12241 aggttcgttc aaaaaccgcc cgctgcagcg aactgatcgc aatgctaagt tgctgtaaca
    12301 ccgggttatg caccgactgc agcaccgcct cgtggtagcg aatatccgct tcgttaaacg
    12361 cttcgcggtc ctgattgttg gcaatcatct cgttcagcgc cgattcaatc tgcgccagat
    12421 cgctggaggt cgcgcgctct gctgcccaac gggcaatcgc cggttccacc agatttcgca
    12481 cttcgctcat ggcactgata agccgtgggt cgtagtcgtt ttccagcacc cattgcagta
    12541 cgtcagtgtc gaggtaattc cactggttac gcggtgccac aaacgccccg cgataacgtt
    12601 tcatttcaat cagccgcttc gccatcagcg aacggaacac ctcacggatg atgttgcgcg
    12661 aggttgcaaa ctcttcacag agctccgcct cagccggaag tggcgagcca ggtacgtatt
    12721 tgccgtgaac gatctgttta cccagcgtaa tgacaatgcg atcggtttta ttgagagtca
    12781 tggagagtcc ttgtgctctg tatgttcttc tctactttac cccgatcgat gcataacgcg
    12841 gcaactttgt agtaccagcg tgatgacgtt cgcgtttgcc gtgcgtgtaa tgtagtacaa
    12901 acttatattg ttgtgctaca atttagatca aaaaaagaac aatgtataaa aaaatgacat
    12961 gcgtcgggca gaaatctgaa aagggatatc aggcgctaaa caggagggaa agaagagtat
    13021 gctttcaacg gcttagctac tcgtttaaaa gattaatcat gaagttgaat tttaagggat
    13081 tttttaaggc tgccggttta ttcccactgg cgctgatgct ttcaggctgt atctcgtatg
    13141 ctctggtttc ccataccgca aagggtagtt caggaaagta tcaatcgcag tcagacacca
    13201 tcactgggct atcgcaggca aaagatagta atggaacaaa aggctatgtt tttgtagggg
    13261 aatcgctgga ttaccttatc actgatggtg ccgatgacat cgttaagatg ctcaatgatc
    13321 cagcacttaa ccggcacaat attcaggttg ccgatgacgc aagatttgtt ttaaatgcgg
    13381 ggaaaaagaa atttaccggc acaatatcgc tttactacta ctggaataac gaagaagaaa
    13441 aggcactggc aacgcattat ggttttgcct gtggtgttca acactgtacc aggtcactgg
    13501 aaaacctaaa aggcacaatc catgagaaaa ataaaaacat ggattactca aaggtgatgg
    13561 cgttctacca tccatttaaa gtgcgatttt atgaatacta ttcacccaga ggcattccgg
    13621 atggtgtttc cgcagcatta ctgccagtga ctgttacgct ggacatcatt actgcaccgc
    13681 tgcaatttct ggttgtatat gcagtaaacc aataatcagt aagcgggcaa acgcgtttat
    13741 gctgtttgcc cgcccacaga ttaattcagc acatacttct caatagcaaa cgccacgcca
    13801 tcttcaaggt tggattttgt gacaaagttc gccatttctt tcaccgaagg aatagcgtta
    13861 tccatcgcca caccgacgcc tgcatattca atcattgcga tgtcgttttc ctgatcgcca
    13921 atcgccatga tttcttccgg tttaatacct aacacgtcgg ccagtgattt cacacccgta
    13981 cctttgttaa cgcgtttatc gaggatttcg aggaagtacg gcgcactttt cagcacggta
    14041 tatttctctt tcacttcctg cggaatacgc gcgatagcct ggtcgaggat ggcgggttca
    14101 tcaatcatca tcactttcag gaactgggta ttggggtcca ttttctccgc ttcgcagaac
    14161 accagcggaa ttgtggcaac gaaggattca tgcaccgtgt agtagctgat atcgcggtta
    14221 gcggtgtaca gcgtggtgcg gtccagggcg tggaaatgag aaccgacttc gcgagagagt
    14281 ttttccagga aacgatagtc gtcatagctg agagcggttt gcgccacggt gctaccatca
    14341 gcggccttct gtaccagcgc gccgttataa gtaatgcagt agtcgcccgg ctgttccata
    14401 tgcagctctt tcaggtagtt gtgcacacct gcatacggac gacccgtcgt tagcacgaca
    14461 ttcacgccac gggcgcgagc tgcggcaatc gcatttttaa cggcgggtga aatggtgtga
    14521 tcgggcagca gaagggtgcc atccatatcg atagcaatga gtttgatagc catgagttcc
    14581 caaggtagat tagttcctga cccatgctaa cgcgattccg ctcaaaaatc agtacaacac
    14641 ccgagggaaa aggggatgca acgcgggttg catgctcccc atttgcttag cggaagagtt
    14701 tccctttcag cagttccatg cctgcggaaa gcagatcgtt attggcttgt gctgacactt
    14761 caccttgcgg tgagagcgca tcaataatct tcggcaattg ctctgccagt aaactggaag
    14821 ccgtactggt atccacgcca agtttttgcc cgagatcgga caccgcattt gtgccgagcg
    14881 ccgattccag ttgctcgccg ctaactgatt gatttctctg ttgattactc aaccaagttg
    14941 agagaatggc ccctaagccg ccactttgca gtttttccag cagcacctga atgccaccct
    15001 gctcctcaac ccaacttaaa atcgcctgat atttccccgc atcgcctttc agaaaggcac
    15061 cgacaacttc atcaaaaagc cccatgataa tcacctgtaa agcgttacgt gttgacccaa
    15121 aaagtataga tctgcgggat gataattgcg gattgcagaa ataaaaaggg cggagatgat
    15181 ctccgccctt ttcttatagc ttcttgccgg atgcggcgtg aacgccttat ccggcctaca
    15241 aaatcatgca aaatcaatat attgcaggag tctcgtagga ctaataagcg tagcgcatca
    15301 ggcacgctcg catggttaac gccattaaat atcaatattc gccgctttca gggcgttctc
    15361 ttcaataaac gcacgacgcg gttctacggc gtcgcccatc agcgtggtga acaactggtc
    15421 agcggcaatc gcatccttaa cggtaacgcg cagcatacga cggctttccg ggtccatggt
    15481 ggtttcccac agctgttccg ggttcatctc gcccagacct ttataacgct ggatggagag
    15541 gccacgacgg gactctttca ccagccagtc cagcgcctgc tcgaagctgg ctaccggctg
    15601 acgacgctcg ccacgttcga taaacgcatc ttcttccagc aagccacgca gtttctcacc
    15661 cagcgtgcag atacgacgat attcgccacc ggtgataaac tcgtgatcca gcggatagtc
    15721 agtatccaca ccgtgggtac gcacgcgaac aatcggctcg aacaggtttt gttcggcatt
    15781 ggtgtggaca tcaaacttcc actggctgcc gtgctgttct ttgtcgttca gttcgctgac
    15841 cagcgcgttc acccagcggg taacggtctg ctcatcagaa aggtcagctt ccgtcaacgt
    15901 cggctgatag ataagctctt tcagcattgc tttcggataa cgacgctcca tgcggttgat
    15961 cattttctgc gtcgcgttgt actcagatac cagtttctct aacgcttcgc cagccaatgc
    16021 cggtgcactg gcgttggtgt gcagcgttgc gccgtccagc gcgatagaga tctggtactg
    16081 atccatcgct tcgtcgtctt taatgtactg ttcctgcttg cctttcttca ctttgtacag
    16141 cggcggctga gcgatgtaga cgtgaccgcg ttcaacgatt tccggcatct gacgatagaa
    16201 gaaggtcaac agcagcgtac gaatgtgcga gccgtcgacg tccgcatcgg tcatgatgat
    16261 gatgctgtga taacgcagtt tgtccgggtt gtactcgtca cgaccgatac cacagccaag
    16321 cgcggtgata agcgtcgcca cttcctgaga agagagcatc ttatcgaagc gcgctttctc
    16381 gacgttaagg attttaccct tcagcggcag aatcgcctgg ttcttgcggt tacgcccctg
    16441 cttcgcagag ccgcccgcgg agtccccttc caccaggtac agttcggaaa gcgccggatc
    16501 gcgttcctgg cagtctgcca gtttgcccgg caggccagct aagtcgagcg cacctttacg
    16561 gcgggtcatt tcacgcgcgc gacgcgccgc ttcacgggca cgggcagcat cgataatttt
    16621 gccaaccacg attttcgcat cagttgggtt ttccagcagg tattccgcca gcagttcgtt
    16681 catctgctgt tcaaccgccg atttcacctc agaagaaacc agtttgtctt tggtctggga
    16741 ggagaatttc gggtccggca cttttacgga aacgaccgca atcaggcctt cacgcgcatc
    16801 gtcaccggtg gcgctgactt tggctttttt gctgtagcct tctttgtcca tgtaggcatt
    16861 cagggtacgg gtcatcgccg cacggaagcc tgccaggtga gtaccgccgt cacgctgcgg
    16921 aatgttgttg gtaaagcagt agatgttttc ctggaagcca tcgttccact gcagcgccac
    16981 ttcaacgcca ataccgtctt tttcggtgga gaagtagaag atatttgggt ggatcggcgt
    17041 tttgttcttg ttcagatatt caacgaacgc tttgatgccg ccttcatagt ggaagtggtc
    17101 ttctttgccg tcacgcttgt cgcgcagacg aatggaaacg ccggagttga ggaacgacaa
    17161 ctcacgcaga cgtttcgcca gaatttcata ttcgaactcg gtcacattgg tgaaggtttc
    17221 gaggctgggc cagaaacgca ccatggtgcc ggttttttca gtctcgccgg taaccgccag
    17281 cggagcctgc ggtacaccgt gttcgtagat ctgacggtga attttaccct cgcgctgaat
    17341 aaccagctcc agtttttgcg acagggcgtt tactaccgaa acaccaacgc cgtgcagacc
    17401 gccggacact ttataggagt tatcgtcaaa tttaccgcct gcgtgcagaa cggtcatgat
    17461 cacttccgcc gccgatacgc cctcttccgg gtgaataccg gtcggaatgc cgcgcccgtc
    17521 atcctgtaca gagacagagt tatcggcgtg aatggtgacg ataatttctt tacagtgacc
    17581 cgcgagcgct tcgtcgatag cgttatctac cacctcgaat accatgtggt gcagaccggt
    17641 gccgtcatcc gtgtcgccga tatacatacc cgggcgctta cgcaccgcat ccagcccttt
    17701 caggactttg atactggagg agtcataaga attcgacatc aacgtttctc gctcatttat
    17761 acttgggtta atccgttatt ttaccctttt ccacggtaaa catcttcgaa ttttcgtccg
    17821 acatgtctat aacgtgttca gcactgatcg cgctgacaaa gacctgtgat tgcgtcgctt
    17881 ttaagcggct ggcaagcaac ccgcgacgct catcatcaag ctcagaggca aaatcatcta
    17941 tcaggtagag acaccgccgc ccgctttcac gagtgaggaa ctctccttgc gccagacgta
    18001 aggcgcacat caacagctta agctgcccac gcgataaggt atcttccacc ggcgcaccgt
    18061 cggcgcgaat gcgtaagtcc gctttatgcg ggccatgcgc ggtgtaggtt agctggcgat
    18121 cgcgttcaaa attacgttcc agcacctcag cgtactctgt ctctttctcc cagccgcgct
    18181 ggaaagagaa agtcagagaa aactcaggga gaaattgctt acaggtatca tccatatcgg
    18241 ccgcgatacc ggcgctatac tccgcgcgcc aggtgctgat ttgctccgcc agcgggatca
    18301 gttctttatc ccacgggcgt agctgttcgt aacgagtcac ctggcgcagc gccgcattgc
    18361 gctgcttgag caatcgcttg agattgctcc aggcggtgaa aaatctaggt tcgttgtgaa
    18421 agcatcccca gtcgaggaat gctcttctgt atttggggcc gccgttgagt aaagtaaacc
    18481 cttctggcgt tatcaactgc atcggcatca ggtgcgccag ttccgcgact ttatgcccgt
    18541 ccgtaccgtc aatacggact ttgctgtcgc cctgtttgtc tttggttaag ccaatcgccg
    18601 tctcgcgctc ttcgccctgt aatcgcccgt ggagaacaaa tgcctcctgc tcatggcgaa
    18661 tgacgcgacc aatctgcaaa ctgcgaaacg cccgaccatg gccgagcgta tagatggctt
    18721 ccagcacgct ggttttgcca ctgccgttgg cacctaccag aaagttaaag ccgggagata
    18781 aagcgagatc cgcggtttca atattgcgga aatcgcggat caacaagcgg gtgagggaca
    18841 ttacagtctc attggcatga caacataggc agccgactgt gatgcggcat cttcaatctg
    18901 cacgctggaa accgaatcgg tcagcatcat gcggacgttt tcgcatttca gcgcgttcag
    18961 aacatccagc acatagctga cgttgaagcc gatttccatc tccgcaccgc tataggtaac
    19021 gtcgaggatc tcttccgctt cttcctgttc cgggttgttg gcagtaattt tcagctggtt
    19081 ttcgctgaca tacaggcgca caccgcggaa tttctcgtta gagagaatcg ccgcgcgagc
    19141 aaacgcctgc ttgagcagat cgcagcctgc ttccagatgt ttgtccgggt tcttcggcag
    19201 aacgcgacgg taatccggga agcgaccatc caccagtttc gaggtgaaaa taaagtcgcc
    19261 aacgtgggca cgaatgttat tgctgccaat ctgcacacgc agcagattgt cgccgccgtc
    19321 gagcatacgc atcagttcaa tcacgccttt acgcggtacg atcaccgaat ggcttggcaa
    19381 agattgacca attggcattg aacagaccgc cagacggtgg ccgtcggttg ccacggtgcg
    19441 cagttcttca ccttcggttt caaacagcat accgtttaag taatagcgaa catcctgatg
    19501 cgccatcgaa aactgggttg cttcaatcag acgcttcatc gtcgcctgcg gcagggtaaa
    19561 ttcgacttcg ctctgccagt catcgaggtt cgggaaatcc gccgctggca gggtagacag
    19621 cgaaaaacgg ctacgcccgg agcgcaccag catccgttca ccttccagct gcacggcaat
    19681 ttccgcgcct tcaggcagac cacggcagat atcaaagaat ttgcgcgccg gaacggtcgt
    19741 cgctcctggc tcgtgtggct gaaccagcgc aacgcgtgcc accatttcca tctcgagatc
    19801 agtaccggtc agcgacaacg taccgtcagc aacctgtaac agcagattac cgagaatcgg
    19861 tagcgtagga cgaccaccta acggaccgct cacctgttgt agcggtttta ataaatgctc
    19921 acgttctacg gtaaatttca taggtttacg atgacaatgt tctgattaaa tttgaaaaat
    19981 cttctttgat atcgtggctc tcttcacgca actgctcgat cttacggcag gcatgaagca
    20041 ccgtcgtgtg gtcacgccca ccaaacgcat cgccaatctc tggcagactg tggttagtca
    20101 gctctttcgc tagcgccatc gccatctggc gcggacgcgc caccgagcgg gatcgacgct
    20161 tggaaaggag atccgcgact ttgatcttgt agtactccgc caccgtcttc tgaatattgt
    20221 cgatggtgac cagtttttcc tgcaatgcca gcaagtcgcg cagcgcctca cgcacgaagt
    20281 cgatggtgat cgcccgtccg gtaaagttgg cgttagcaat aacgcggttc agcgcccctt
    20341 ccagctcacg tacgttagat cgtagacgct tggcgataaa gaacgccact tcacccggca
    20401 aacgaatgtc attttcgtcg gcctttttca tcaggatcgc cacacgggtt tccagctctg
    20461 gcggttcgat cgccacagtc agtccccaac cgaagcggga tttcaaacga tcctcaacgc
    20521 cgttgatctc tttcggatag cgatccgagg tgagaatgat ctgttgatta ccttccagca
    20581 gggcgttgaa ggtgtggaaa aactcttcct gagatcgttc tttattagca aaaaactgaa
    20641 tatcgtcgat cagcagtgca tctacggaac ggtagtagcg tttaaactct tcgatcgcgt
    20701 tgttttgcag ggctttaacc atgtcctgaa caaagcgctc ggagtgcata taaaccactt
    20761 tggcattcgg cttgcgcgcc ataatgccgt tacccaccgc atgcagcagg tgagttttac
    20821 ccagacccgt gccgccataa aggaacaacg ggttataggc accgccaggg ttatccgcca
    20881 cctggcgagc cgccgcgcgc gccagttggt tagatttacc ttcaacgaag ttatcaaacg
    20941 tgtgtttgac gtttacgtta gaacgatagg tcggttctgc cggggccggg acgttatccc
    21001 aacctgagcg cgtagaaggc gcagcacgtt gcggctgcgt ttgcgccacc tgtgcagggg
    21061 ccgcgacgtt gctcgtcact gccgcttgtg gcgtttgcgt caccggtttg gtgccgactt
    21121 caaaacgcag ctgtggggca tccgctccgc agaaactggt tagcagtcca ttgatattat
    21181 taaggtactt gtcccgtacc caatcgagga caaaacggtt tggcgcgtac agggacagcg
    21241 tgttatcgct cagttccgcc tgcaatgggc gtatccacat actgaattct gtggctggta
    21301 actcatcctg caatcgggca agacactgct gccaaagcga aagtgacacg gcggactcca
    21361 ctcgaacaaa agtcgataat gactaaggct gaaacattca tgattgttga cgtacgtcga
    21421 aaagaccctg cttgagggtg acgcacgaac cgctgtctgc ggttatatgc ccgatcaaga
    21481 tcctgcaaaa cgatcgggac cgcggatcat agcctaaact gcgcaagaga tcttctgttt
    21541 ctcacagatt ttccctattt atccacagga ctttccagaa ctcgctaagt gtaaacgatc
    21601 ctgccgcgag gcgggcacga tttacgccgc atattggaaa atttaatgac catagacaaa
    21661 aattggctta atcgatctaa taaagatcca ggacgatcct tgcgctttac ccatcagccc
    21721 gtataatcct ccacccggcg cgccatgctg gtttccactg gtgtgaggcc gtacattttc
    21781 cctgcgaaaa ggtgcggaaa agcgcggtaa ataaggaaag agaattgact ccggtgtgta
    21841 caattattac aatccggcct ctttaatcac ccatggcttc ggtgtccatc gtttcatttt
    21901 tcggcggata tccaataaag ccattgaatt tattcaagtt taggtagaaa tcgccatgaa
    21961 acgcactttt caaccgtctg tactgaagcg caaccgttct cacggcttcc gtgctcgtat
    22021 ggctactaaa aatggtcgtc aggttctggc acgtcgtcgt gctaaaggcc gcgctcgtct
    22081 gaccgtttct aagtaataaa gctaacccct gagtggttaa gctcgcattt cccagggagt
    22141 tacgcttgtt aactcccagt caattcacat tcgtcttcca gcagccacaa cgggctggca
    22201 cgccgcaaat taccattctc ggccgcctga attcgctggg gcatccccgt atcggtctta
    22261 cagtcgccaa gaaaaacgtt cgacgcgccc atgaacgcaa tcggattaaa cgtctgacgc
    22321 gtgaaagctt ccgtctgcgc caacatgaac tcccggctat ggatttcgtg gtggtggcga
    22381 aaaaaggggt tgccgacctc gataaccgtg ctctctcgga agcgttggaa aaattatggc
    22441 gccgccactg tctcctggct cgcgggtcct gatagccctc attcgggtct atcaacgcct
    22501 gattagtccg ctactcgggc cgcattgtcg tttcactcca acctgttcaa gctacggaat
    22561 tgaggcattg cgcaggtttg gagtgataaa aggcagttgg ttgacggtga aacgcgtatt
    22621 aaaatgccac cctttacacc ctggtggtga cgatcccgtc ccgcccggac catttgatac
    22681 cagagaacac taacgatgga ttcgcaacgc aatcttttag tcatcgcttt gctgttcgtg
    22741 tctttcatga tctggcaagc ctgggagcag gataaaaacc cgcaacctca ggcccaacag
    22801 accacgcaga caacgaccac cgcagcgggt agcgccgccg accagggcgt accggccagt
    22861 ggccagggga aactgatctc ggttaagacc gacgtgcttg atctgaccat caacacccgt
    22921 ggtggtgatg ttgagcaagc tctgctgcct gcttacccga aagagctgaa ctctacccag
    22981 ccgttccagc tgttggaaac ttcaccgcag tttatttatc aggcacagag cggtctgacc
    23041 ggtcgtgatg gcccggataa cccggctaac ggcccgcgtc cgctgtataa cgttgaaaaa
    23101 gacgcttatg tgctggctga aggtcaaaac gaactgcagg tgccgatgac gtataccgac
    23161 gcggcaggca acacgtttac caaaacgttt gtcctgaaac gtggtgatta cgctgtcaac
    23221 gtcaactaca acgtgcagaa cgctggcgag aaaccgctgg aaatctcctc gtttggtcag
    23281 ttgaagcaat ccatcactct gccaccgcat ctcgataccg gaagcagcaa cttcgcactg
    23341 cacaccttcc gtggcgcggc gtactccacg cctgacgaga agtatgagaa atacaagttc
    23401 gataccattg ccgataacga aaacctgaac atctcttcga aaggtggttg ggtggcgatg
    23461 ctgcaacagt atttcgcgac ggcgtggatc ccgcataacg acggtaccaa caacttctat
    23521 accgctaatc tgggtaacgg catcgtcgct atcggctata aatctcagcc ggtactggtt
    23581 cagcctggtc agactggcgc gatgaacagc accctgtggg ttggcccgga aatccaggac
    23641 aaaatggcag ctgttgctcc gcacctggat ctgaccgttg attacggttg gttgtggttc
    23701 atctctcagc cgctgttcaa actgctgaaa tggatccata gctttgtggg taactggggc
    23761 ttctccatta tcatcatcac ctttatcgtt cgtggcatca tgtacccgct gaccaaagcg
    23821 cagtacacct ccatggcgaa gatgcgtatg ctgcagccga agattcaggc aatgcgtgag
    23881 cgtctgggcg atgacaaaca gcgtatcagt caggaaatga tggcgctgta caaagctgag
    23941 aaggttaacc cgctgggcgg ctgcttcccg ctgctgatcc agatgccaat cttcctggcg
    24001 ttgtactaca tgctgatggg ttccgttgaa ctgcgtcagg caccgtttgc actgtggatc
    24061 cacgacctgt cggcacagga cccgtactac atcctgccga tcctgatggg cgtaacgatg
    24121 ttcttcattc agaagatgtc gccgaccact gtgaccgacc cgatgcagca gaagatcatg
    24181 acctttatgc cggtcatctt caccgtgttc ttcctgtggt tcccgtcagg tctggtactg
    24241 tactatatcg tcagcaacct ggtaaccatt attcagcagc agctgattta ccgtggtctg
    24301 gaaaaacgtg gcctgcatag ccgcgagaag aaaaaatcct gattcggtga gttttcgcta
    24361 aaataagggc ggtcagttga ccgccttttt tcttttcgta gggcggataa gcaccgcgca
    24421 tccgccacac aaagcaacag gaacatcatg agcgataatg acactatcgt agcccaggcc
    24481 acacctccgg gacgtggcgg cgttggcatc ctgcgcatct ccggcttcaa agcccgtgaa
    24541 gttgccgaaa ccgtgctggg taaactgcct aagccgcgct acgccgatta tcttccgttt
    24601 aaagacgccg acggcagcgt gctcgatcag gggattgcgc tatggttccc tggcccgaac
    24661 tcgttcaccg gcgaagatgt gctggaactg caaggtcatg gcggtccggt gatcctcgac
    24721 ctgctgttaa aacgcattct gaccattccc ggcctgcgga ttgctcgccc tggtgagttt
    24781 tccgaacgcg cgtttcttaa cgataaactt gacttagccc aggccgaggc gattgccgat
    24841 cttatcgacg ccagttcgga acaggcggcc cgttcggcac ttaactcgct gcaaggcgca
    24901 ttctccgcac gggttaatca tctggtagaa gccctcaccc acttgcgcat ttacgtcgaa
    24961 gcggcaattg atttccctga tgaagagatc gatttcctct ccgacggcaa aattgaagcc
    25021 cagcttaatg acgtgatggc cgatctcgat gcagtgcgtg ctgaagcacg tcagggtagt
    25081 ttgttgcgcg aagggatgaa agtagtgatt gccggacgtc ctaatgccgg taaatcgagc
    25141 ctgttaaacg cgctggcggg ccgtgaagcg gcaatcgtaa ccgatatcgc cggaaccacg
    25201 cgtgacgtgc tgcgtgagca tatccacatt gacggaatgc cgctgcatat catcgacact
    25261 gccgggctac gtgaggccag tgacgaagtg gaacgtattg gtatcgagcg cgcgtggcag
    25321 gaaattgaac aggccgaccg cgtgctgttt atggtcgatg gcaccacgac agacgccgtt
    25381 gatccggcag agatctggcc ggaatttatc gcccgtctgc ccgcgaaact gccgatcacc
    25441 gtggtacgca ataaagccga tatcaccggc gaaacgctgg gaatgagtga agtgaacggt
    25501 cacgcgttaa ttcgtctctc ggcaaggact ggtgaaggcg tggacgtgct gcgtaaccat
    25561 ctcaaacaga gcatgggctt tgacaccaat atggaaggcg gcttcctggc gcgccgtcgc
    25621 cacttacagg cgctggaaca ggcagcggag catttgcagc aaggtaaagc gcaactgttg
    25681 ggagcctggg caggtgaact gctggcggaa gagttgcgcc tggcacagca gaacttaagc
    25741 gaaatcaccg gggaatttac ttcagacgac ctgctggggc ggattttctc cagcttctgt
    25801 attggtaagt aaccgcgctt acgaagccgt attctgactg tcagatgcgg cttcgcttca
    25861 ttgttagcac tcctgttatg cctcaaccct tttttaaaca ttaaaatt
//
LOCUS       DADMGC010000050        25888 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.050, whole genome shotgun sequence.
ACCESSION   DADMGC010000050 DADMGC010000000
VERSION     DADMGC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.050"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            347..640
                     /locus_tag="JNZ73_002012"
     CDS             347..640
                     /locus_tag="JNZ73_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732306.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            712..1371
                     /locus_tag="JNZ73_002013"
     CDS             712..1371
                     /locus_tag="JNZ73_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAY8732307.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1448..1909
                     /locus_tag="JNZ73_002014"
     CDS             1448..1909
                     /locus_tag="JNZ73_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HAY8732308.1"
                     /translation="MAEHLMSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKDLPAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="JNZ73_002015"
                     /pseudo
     CDS             complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="JNZ73_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3389..3808
                     /gene="umuD"
                     /locus_tag="JNZ73_002016"
     CDS             3389..3808
                     /gene="umuD"
                     /locus_tag="JNZ73_002016"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY8732309.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQRL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3808..5074
                     /locus_tag="JNZ73_002017"
                     /pseudo
     CDS             3808..5074
                     /locus_tag="JNZ73_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            complement(5120..5650)
                     /gene="dsbB"
                     /locus_tag="JNZ73_002018"
     CDS             complement(5120..5650)
                     /gene="dsbB"
                     /locus_tag="JNZ73_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HAY8732310.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5796..7337)
                     /gene="nhaB"
                     /locus_tag="JNZ73_002019"
     CDS             complement(5796..7337)
                     /gene="nhaB"
                     /locus_tag="JNZ73_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HAY8732311.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREGVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7558..8277
                     /gene="fadR"
                     /locus_tag="JNZ73_002020"
     CDS             7558..8277
                     /gene="fadR"
                     /locus_tag="JNZ73_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HAY8732312.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8329..9861)
                     /locus_tag="JNZ73_002021"
     CDS             complement(8329..9861)
                     /locus_tag="JNZ73_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HAY8732313.1"
                     /translation="MATIDSINKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHTCYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10191..11489
                     /locus_tag="JNZ73_002022"
     CDS             10191..11489
                     /locus_tag="JNZ73_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HAY8732314.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11499..12569
                     /gene="dadX"
                     /locus_tag="JNZ73_002023"
     CDS             11499..12569
                     /gene="dadX"
                     /locus_tag="JNZ73_002023"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HAY8732315.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12737..14163)
                     /locus_tag="JNZ73_002024"
                     /pseudo
     CDS             complement(12737..14163)
                     /locus_tag="JNZ73_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109184.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14773..16509)
                     /locus_tag="JNZ73_002025"
     CDS             complement(14773..16509)
                     /locus_tag="JNZ73_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAY8732316.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16604..17518)
                     /gene="ldcA"
                     /locus_tag="JNZ73_002026"
     CDS             complement(16604..17518)
                     /gene="ldcA"
                     /locus_tag="JNZ73_002026"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HAY8732317.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            17618..18229
                     /gene="emtA"
                     /locus_tag="JNZ73_002027"
     CDS             17618..18229
                     /gene="emtA"
                     /locus_tag="JNZ73_002027"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HAY8732318.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18231..18965)
                     /locus_tag="JNZ73_002028"
     CDS             complement(18231..18965)
                     /locus_tag="JNZ73_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HAY8732319.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19166..19420
                     /locus_tag="JNZ73_002029"
     CDS             19166..19420
                     /locus_tag="JNZ73_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAY8732320.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19470..21424)
                     /locus_tag="JNZ73_002030"
                     /pseudo
     CDS             complement(19470..21424)
                     /locus_tag="JNZ73_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(21450..22304)
                     /locus_tag="JNZ73_002031"
     CDS             complement(21450..22304)
                     /locus_tag="JNZ73_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HAY8732321.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22304..23113)
                     /locus_tag="JNZ73_002032"
     CDS             complement(22304..23113)
                     /locus_tag="JNZ73_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAY8732322.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23123..23881)
                     /locus_tag="JNZ73_002033"
     CDS             complement(23123..23881)
                     /locus_tag="JNZ73_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8732323.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23878..24858)
                     /locus_tag="JNZ73_002034"
     CDS             complement(23878..24858)
                     /locus_tag="JNZ73_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAY8732324.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVSV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGELMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISP
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24858..25880)
                     /locus_tag="JNZ73_002035"
     CDS             complement(24858..25880)
                     /locus_tag="JNZ73_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HAY8732325.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6450 a         6372 c         6727 g         6339 t
ORIGIN      
        1 atggcctttg ctgaactcaa cattgacatt cttacccgca gcaaagctcg tggaggttag
       61 cagtgcaagc agcaatacag ccttactgat gctcttgatt ttcattggta gtctcctgtt
      121 cagaatgagt acgatgaact gttataatat aacaatccct tacgggagaa ttccagtggc
      181 ttttctgcgt tcggtaaaat cccggcgcgg cattttttgc cggggggatt acttataaag
      241 gggcatggac tcaacaaacc gcagaaatct acagctggct ggcttgtggc gcgggtttca
      301 ttgatgttag cttatgccaa aacctggcat cttaaagagt aaaagtatgt tttgtgtgat
      361 ttatcgaagc agcaagcgtg accagaccta tttatatgtc gaaaaaaaag acgatttttc
      421 gcgtgttcct gaggaactga tgaaaggttt tggtcagcct cagttagcga tgattctgcc
      481 gttggatggg cggaagaaac tggtcaatgc tgatattgaa aaggttaaac tggcattaac
      541 cgagcaaggt tactatttgc agttaccgcc accacccgaa gatttgctga agcaacatct
      601 ttccgtcatg gggcagaaaa cagacgacac taacaaataa ccgatatccg gcggtggcat
      661 tatctttgtc ggcgcgggtt ttcatatcca cgataaggtg aggggaacgt tatgtatcaa
      721 catcacaact ggcaaggtgc gctgctggat tatccggtga gtaaagtagt ctgtgttggc
      781 agtaactatg ccaaacatat taaagagatg ggcagcgcag tgcccgaaga gccagtgctg
      841 tttattaaac cagaaacggc actgtgcgat ctgcggcagc cattggcgat cccatccgat
      901 ttcggttcag ttcatcatga agtcgaactg gcggtgttga ttggcgcgac gctgcgtcag
      961 gcaacggaag agcatgtccg caaagccatt gccggttacg gcgtggcgct tgatctgaca
     1021 ttgcgtgatg ttcagggaaa aatgaagaaa gccgggcagc cgtgggaaaa ggctaaagcg
     1081 tttgataact cttgtccgct ttccgggttt atccccgcgg cggaatttac cggcgatcca
     1141 caaaatacaa cgctgggcct gagcgtaaac ggcgaaaaac gccagcaagg tacgactgcg
     1201 gacatgatcc ataaaatcgt tccgttgatc gcttatatga gcaagttttt taccctcaag
     1261 gccggtgacg ttgtgttgac aggcacgcct gatggcgtcg gcccgttgca aagcggtgat
     1321 gagctgacag tcactttcga tgggcattct ttgacaactc gcgttttgta atactttttg
     1381 ccgccagaaa gcggcggcaa aacttgcatc gctgtgccag actggttata aggtgcgttt
     1441 taacgtaatg gcggaacacc tgatgagcga tgtacctttc tggcaaagta aaaccctgga
     1501 cgaaatgagc gatgcggaat gggagtcgtt gtgtgatggt tgcggtcagt gttgcctgca
     1561 taaactgatg gatgaagaca ccgacgaaat ctacttcact aacgtcgcct gtcgccagct
     1621 caatattaaa acctgtcaat gtcggaacta cgaacgtcgt ttcgagtttg aacccgactg
     1681 cattaaatta acccgtgaaa atctgccaac attcgaatgg ctgccaatga cctgcgctta
     1741 tcggttgctg gcggaaggta aagatttacc tgcgtggcat ccgctactta ctggttcgaa
     1801 agcggcaatg catggtgaac gtatctctgt gcgtcatatc gcagtgaaag aatcagaagt
     1861 gattgactgg caggatcata tcttaaataa acctgactgg gcgcagtgat aaatttaata
     1921 gatgctgatg ggggctttct taatatacgt ctggatgctc gccatttcgt agccctgttt
     1981 ttgaaagcgc tctgtcataa tgagagttac ccgatacctt attatcaaca ggtaactctc
     2041 aatctttaaa aaacgtctgt gtttttaaat gtgataaacc ttataccttg agtacatgga
     2101 gagaataatc gcttatcaga cttcaggtac ttcaaagagt gtctttttac cgtgtctttt
     2161 ctgatactca ttacaggtgt taatcatttt tttggccgct tcttttagca aagaaagcat
     2221 taaatcatca taatcaacat agaatctggt tgtttcagtt tccgttttta tctccccgat
     2281 ggcggctatt tcggtggtta atttcaattt ggcggcatcg atatctttat tcgcttgttt
     2341 aaccgtgtta gacagggtgg taaagaaact ctgcacagat tttaacttgt tcttcaattc
     2401 tggaatcagt ttcccttcaa ctacgcccgc agcaatagaa taggaaatga ttaaaccaaa
     2461 tggaccggcg acgacatcgg ctgcggcacc ggcatacgct tccttcctga ttttatctac
     2521 ctgtgactgg aaatagctgc ttttttctga aaaatcattg gttaactggc tatctaacgc
     2581 cagcagtttc ccggaagcgt tgttgaaact ttgtgagctt accagcaggg atttttgcgc
     2641 ttcattcagc ttcgtgatgc cgtcatccag taccttaatg aggggcggat gctttcttct
     2701 cattgtactc atcaaatagc aaaatatacg ctgcgagcaa ttgcgtcgca acaccacacc
     2761 attcatacac tgtttgggtt gcttcaaaat acttatcctg gctatccata agtaaggttt
     2821 taatatcgcc gactaaaacg gaggctgcct gtgaatactc ctgtttaaag cgacttaact
     2881 cttttatggt ttcatcaaag gtctgccagg ggatgacctg atcgagatat ttattataaa
     2941 gatctaatgc tccatctgcg gtttcgattg cgtttttaac tacttctacc gttttatctg
     3001 caacgatttc agtcataatc attcgcctct ttaaatatat aaattgtaat gaaactcctg
     3061 ttttacaact attaataaat tttacttcat ctaattcata gttagccggg cgggatgcgt
     3121 caatgtcttt atttctatta atatgataaa tatcaaacaa tgtttaatgt cattatggcg
     3181 aatgcttcta ttctattttt ttgccgggtg atatttttca tttctgctgg atgagcgtcg
     3241 tcgccagaag gccacgtgag cacaggataa gagaacgaaa aatcagcagt ctatgcagcg
     3301 acaaatattg atagcctgaa tcagtattga tctgctggca agaacagact actgtatata
     3361 aaaacagtat agcttcaggc agattattat gttgtttatc aagcctgcgg atctccgcga
     3421 aattgtgact tttccgctat ttagcgatct tgttcagtgt ggctttcctt caccggcagc
     3481 agattacgtt gaacagcgca tcgatctgaa tcaacggttg atccagcatc ccagcgcgac
     3541 ttacttcgtc aaagcaagtg gtgattctat gattgatggt ggaattagtg acggtgattt
     3601 actgattgtc gatagcgcta ttaccgccag ccatggtgat attgtcatcg ctgctgttga
     3661 cggcgagttt acggtgaaaa aattgcaact acgcccgacg gtacagctta ttcccatgaa
     3721 cagtgcgtac tcgcccatta ccatcagtag cgaagatacg ctggatgtct ttggtgtggt
     3781 gatccacgtc gttaaggcga tgcgctgatg tttgccctct gtgatgtaaa cgcgttttat
     3841 gccagctgtg agacggtgtt tcgccctgat ttatggggta ccggtggttg tgctatcgaa
     3901 taatgacggt tgcgttatcg cccgaaacgc tgaggcaaag gcgctgggcg ttaaaatggg
     3961 cgatccctgg ttcaaacaaa aagatctgtt tcgtcgctgt ggtgtggttt gctttagcag
     4021 caattatgag ctttacgcag acatgagcaa tcgggtgatg tcgacgctgg aagaactatc
     4081 gccccgcgtc gagatttaca gtattgatga agcattctgc gatctgacag gtgtgcgtaa
     4141 ttgtcgcgat ctgactgatt ttggcagaga aattcgcgca acggtgctac aacgtaccca
     4201 tcttactgtt ggtgtgggga tcgcccagac caaaacgttg gctaagcttg ccaatcatgc
     4261 ggcaaaaaaa tggcagcggc agacgggtgg ggtggtggat ttatcaaatc tggaacgcca
     4321 gcgtaaatta atgtctgctc tccccgtgga tgacgtctgg gggattggac ggcggatcag
     4381 caaaaaactg gacgcgatgg ggatcaaaac cgttctcgat ttggcggata cagatatccg
     4441 gtttatccgt aaacatttta atgtcatgct cgaaagaacg gtgcgtgaac tgcgcggcga
     4501 accctgtttg caactggaag agtttgcacc gacgaagcag gaaattatct gttcccgctc
     4561 gtttggtgaa cgcatcacgg attatacgtc gatgcggcag gccatttgta gttacgctgc
     4621 ccgggcggcg gaaaaacttc gcagtgagca tcaatattgt cggtttatct ccacatttat
     4681 taagacgtca ccatttgcgc tcaatgaacc ttattacggc aatagcacgt cggtaaaact
     4741 gctgacgccc actcaggaca gcagggatat cattaacgct gctacgcgat ctctggatgc
     4801 catctggcaa gcgggccatc gctaccaaaa agcgggcgtg atgctggggg atttcttcag
     4861 tcagggagtc gcgcagctca atttattcga tgacaatgca ccgcgccccg ggagtgagca
     4921 attgatggcg gtaatggata ctctgaatgc taaagagggc agaggaacac tctattttgc
     4981 cgggcagggg atccaacagc aatggcagat gaagcgagct atgctttcac cacgttatac
     5041 aacgcgaagt tctgatttac tgcgggtcaa ataaatatag cggcaggaaa aaagcgttcc
     5101 cgcaggagcg ccgaatggat tagcgaccga acagatcacg ttttttcgct ttaaacggct
     5161 gggaaatcac caccagcacc gcgacaatca ggtaagcgat aaaaataccg agcagccact
     5221 gcggcatttc catacctaaa aaatcccact gacgctcggc gcaatcgcca gaggcgacaa
     5281 acacttgcgg cacccattta tccagcggca gccattccgg gaaacgaacc ataaaatcac
     5341 aggtggcaaa tggcgaagga tagagctgaa gcatggtgtg ctcgtaagtt aactgcacac
     5401 cgcggaacgc actatacaac cagataacca tcgctacata acgcagcgga gttttcgggg
     5461 cgatcgcgcc aatcagcgca gcacccagaa cgccgaataa cgcgcagcgt tcataaatac
     5521 agagcacgca aggtttcagt aacatcacat gctggaacca cagcgccgtc agttccagtg
     5581 ccagagcagt aaacgccatc aacagccacg cgccccggcc ttgtgaacat tggttcaaaa
     5641 atcgcaacat aatcatttcc ctgcaatatg catagagtgc gcagtttaaa ccaattcatt
     5701 cgctgcgcca ccaggaggca gcaaaaatag gacgcaattg gacaattatc gggcgaaaag
     5761 gcaaaccggg catttatatg cccggtaagt tgttatcaaa gcgttgctat ccagcccatt
     5821 tgcataaacc attcggttac aggggcaagc gtaaactcga cgcagagcaa gccgacgagt
     5881 gtcagtacga gggtgtaagg cagggccatc cacaccatgc ggccataaga gaggcgaatc
     5941 aatggcgcga gtgcagaggt cagcaggaac aggaacgcag cctgaccgtt cggcgtagcg
     6001 acagagggca gattggtacc ggtattaatg gcgaccgcca gcagctcgta ttgcttcaac
     6061 gtgatagcgc cactttccat tgccgctttc gcttcgttga tataaatcgt ccccacgaag
     6121 acgttatccg aaatggatga cagcagaccg ttgaaaatat agaacagcga cagctgagca
     6181 tgttcagatg cctgcaacac aaactgaata atcggcgaaa acagttgttg gtcgataatc
     6241 accgcgacta ccgagaaaaa caccgtcaac agtgcggtga atggcagaga ttcggtgaag
     6301 gctttaccga tagcatgttc atcggtgaca ccggtcaatg atgttgccag aataatgact
     6361 gacaaaccaa tcaagccaac ttccgccaga tgcaacgcca gcgcagtcac cagccagacg
     6421 ccaataatcg cctggacaat cagacggatt ttatcctgac gggtacgctg gtggcggctt
     6481 tgatcgtcaa actgttgcaa cacttcgcgg actttctccg gcagcgtttc accgtagcca
     6541 aaccaacgca gcttctctac cagcaggcag gttaacaggc cacaaatcag aaccggaacg
     6601 gtcaccggcg acatgcgcag gaagaaatcg ccaaaatgcc agccagccgc tttagcgatg
     6661 atcaggttct gtggttcgcc caccatggtc attacgccgc ctaatgcggt gccgacaccg
     6721 gcatgcatca tcaggctacg cagaaagcca cggaactgtt ccagaaccac gttgtaatgc
     6781 ttgtcgatat gactatcgtc ttgcaggtcg gtgtcttcgg tacgggaaga ggctacgcga
     6841 tgataaatac cataaaaacc gactgcaacg ctgatcacca ccgccacgac ggttaaggca
     6901 tcgaggaacg cggagaggaa cgctgccgcc acgcaaaaag agagcgacag cagcattttg
     6961 gagcgaatgc ttaacagcaa acgggtaaat atgaacagca acagctgttt cataaaatag
     7021 atacccgcca ccataaacat caataacagc aagacttcaa gatttgccgc caccccttca
     7081 cggacgtgtt ccgcgctggt catgccgatg aataccgctt cgatagccaa cagaccaccg
     7141 gggagtagcg ggtagcattt cagggccatc gccagagtga aaataaattc cgctaccagc
     7201 aaccagcccg cgacgaaagg gctgatgagg aaaattaacg ggtttacgat taagaaaatg
     7261 atgagggcga gtttgtacca gtcgggggac tggcccaaaa aattgcgcca tagcgcgcgg
     7321 ccccaggaga tctccatgat ggtttccctt accttacaaa taatcaatga tgtttttgtg
     7381 tttaaacgca aagcttaacg gtcaggcagg agtgaggcaa gtcttgatag tcaaggggaa
     7441 agagatgcgg aaaatgaagc cttgatccct ttttcttctt tttgtctgct atcagcgtag
     7501 ttagccctct ggtatgatga gtccaacttt gttttgctgt gttatggaaa tctcactatg
     7561 gtcattaagg cgcaaagccc ggcgggtttc gcggaagagt acattattga aagtatctgg
     7621 aataaccgct tccctcccgg gactattttg cccgcagaac gtgaactttc agaattaatt
     7681 ggcgtaacgc gtactacgtt acgtgaagtg ttacagcgtc tggcacgaga tggctggttg
     7741 accattcaac atggcaagcc gacgaaggtg aataatttct gggaaacttc cggtttaaat
     7801 atccttgaaa cactggcgcg actggatcac gaaagtgtgc cgcagcttat tgataatttg
     7861 ctgtcggtgc gtaccaatat ttccactatt tttattcgca ccgcgtttcg tcagcatccc
     7921 gataaagcgc aggaagtgct ggctaccgct aatgaagtgg ccgatcacgc cgatgccttt
     7981 gccgagctgg attacaacat attccgcggc ctggcgtttg cttccggcaa cccgatttac
     8041 ggtctgatcc ttaacgggat gaaagggctg tatacgcgca ttggtcgtca ctatttcgcc
     8101 aatccggaag cgcgcagtct ggcgctgggc ttctaccaca aactgtcggc gttgtgcagt
     8161 gaaggcgcgc acgatcaggt gtacgaaaca gtgcgtcgct atgggcatga gagtggcgag
     8221 atttggcacc ggatgcagaa aaatctgccg ggtgatttag ccattcaggg gcgataatcc
     8281 cttccgttta aagagcaaac ccctcaaacg aggggttttt tgttgttttt acagatttcc
     8341 cattcttggc gggcaacgtt ccagcaactc gatgctgccg tcttcgtttt gctgttcgag
     8401 catcacatca aatccccaca ggcgatgcac atgcttcagg acttctttgc gcccccgatc
     8461 cagcggtgcg cgattatgtg gaatataacg cagcgtcagc gaacggtcgc cgcgcaaatc
     8521 cacgttccag atctgaatat tcggctccag attacttaag ttatattgcg acgataaccg
     8581 gttacggatc tcccgataac cttcttcatt atgaatagcg gaaatctcca gataattatg
     8641 ccgatcatca tccagcacgg tgaagaagcg gaaatcacgc atcactttcg gtgacagaaa
     8701 ctggctgata aaactctcat ctttgaaatc acgcatcgcg aaatgcagcg tttccagcca
     8761 gtcggaaccg gcgatatccg ggaaccagta tttgtcttct tccgttggcg actgacaaat
     8821 ccgtttaata tcctggaaca tggcgaaccc gagggcatac gggttgatgc cgctgtacca
     8881 cgggctgtta taggggggct ggaagaccac attggtgtgg ctgtgcaaaa actccagcat
     8941 aaaacgttcc gttactttcc cttcatcata cagatggtta aggatggtat agtgccagaa
     9001 ggtcgcacag ccttcgttca taacctgagt ctgtttttgc ggataaaaat actggctcac
     9061 cttacgcaca atacgcagga tttcacgctg ccatgattcc agcagtggcg catttttctc
     9121 cataaaatag agcaggtttt cttgtggttc ggacggatag cggcgcgctt cagcaaccgt
     9181 tttctcttcc tcgcgcttcg gcagggtacg ccagagcata ttgacctgac tttgtagata
     9241 ctcttcgcga cttttctgcc gggctttctc ttcttgcagc gagatttttt gcgggcgttt
     9301 gtaccggtcc acgccgtagt tcatcagcgc gtggcacgag tccagaagcc gttctacttc
     9361 atcaacgcca taacgttctt cgcactcggt aatatattta cgggcaaaaa tcagataatc
     9421 gacaatcgaa ctggcgtcgg tccaactgcg gaataagcaa ttgtttttaa agaaagagtt
     9481 atgcccatag caggtgtgtg ccatcaccag cgcctgcata gtaatggtgt tctcttccat
     9541 caagtaagcg atacacgggt tagagttaat gacgatttca taggccagcc cttgctgacc
     9601 gtgcttatac agccgttcag tctcgataaa ctttttaccg aatgaccagt gcggatagtt
     9661 aattggcatg ccgacgctgg aataggcatc catcatctgt tctgaggtta tcacttcaat
     9721 ctggtgcggg taagtatcca gccggtagag tttcgccacc cggtctatct ctgccagata
     9781 aacatccagc aggtcgaacg tccagtcggg tccatcgctc aaacgtgtgg tgtccttatt
     9841 aatagaatcg atcgtcgcca tacgcgcacc tcattgttgt cggcgctctc tgtgtggagc
     9901 acctcatttc aagcatagaa cacctgttaa aaaccgcgtc gccggagaat ttttttcttt
     9961 gcgatttctt attatcagag tgccactaat ccgcttctga acggaatttt atgctggata
    10021 aaaagggcgt tcagcaggag atactaaaga cgccatattg ccgcagagtc agggagatgt
    10081 gagtcagctc accataaaaa agccgtatgt tgaataatat tttcaactga gttatcaaga
    10141 tgtaattaga ttattattct tttactgtat ctaccgttat cggagtggct atgcgagttg
    10201 tcatactggg aagtggtgtg gtaggcgttg ccagcgcctg gtacttaaat caggcaggac
    10261 atgaggtcac cgtcattgat cgggagccgg gggccgccct ggaaaccagt gccgcgaatg
    10321 ccgggcaaat ctcccccgga tatgctgcac cgtgggcagc accaggtgtg cctttaaaag
    10381 cgattaaatg gatgttccag cgccatgcgc cgctggcggt tcgtctcgac ggtacgcagt
    10441 ttcagttgaa atggatgtgg caaatgttac gtaactgcga caccagccac tacatggaaa
    10501 acaaagggcg gatggtgcgt ctggcggaat acagccgtga ttgcctgaaa gcattgcgcg
    10561 ccgaaaccaa tattcagtat gaagggcgtc agggtgggac gctgcaactg ttccgtaccg
    10621 aacaacagta tgaaaatgcg acccgcgata tcgccgtgct ggaagatgcc ggcgtaccgt
    10681 atcagctgct ggaatccagc cgcctggcgg aagtggatcc cgcgctggca gaagtagcgc
    10741 acaaactgac gggcggcctg cagttaccca atgatgaaac cggagactgc cagctgttta
    10801 cccagaatct ggcgcggatg gcagagcagg cgggggttaa attccgcttt aatacgcccg
    10861 ttgaccaact gctttgcgac ggcgagcaaa tctacggcgt gaagtttggc gatgaagtga
    10921 ttaaggccga tgcgtatgtg atggcgtttg gttcttactc gacggcgatg ctcaaaggca
    10981 ttgttgatat tccggtgtat ccgctgaaag gctactcgct gactattcca attgcgcagg
    11041 aagatggtgc gccagtatcc accattcttg atgaaaccta caaaatcgcc attacccgtt
    11101 tcgataaccg cattcgtgtt ggcggaatgg cggaaattgt tggttttaac accgagctgt
    11161 tgcaaccgcg tcgtgaaacg ctggaaatgg tggttcgcga tctctatcca cgcggcggtc
    11221 atgtcgagca ggcgactttc tggacgggtt tgcgcccgat gacgccagac ggtacgccgg
    11281 ttgtcgggcg tacacgcttt aaaaatctgt ggctgaatac cggtcacggc acgctcggct
    11341 ggacgatggc ttgcggttcc ggtcagttgt taagcgatct gctctctggt cgcacgccag
    11401 cgatcccata tgaggatcta agcgtagcgc gctacagccg tggatttacg ccatcacgtc
    11461 cgggccattt acatggcgca cacagctaag gaaacgagat gacccgtccg atacaggcca
    11521 gcctcgatct gcaggcatta aaacagaatc tgtccattgt ccgccaggcc gcgccgcacg
    11581 cgcgcgtctg gtcggtggta aaagcgaacg cttacgggca tggtattgag cgtatctgga
    11641 gcgcgctcgg ggccaccgat ggctttgcat tacttaacct ggaagaggca ataacgttac
    11701 gtgagcgcgg ctggaagggg ccgatcctga tgctggaagg atttttccat gctcaggatc
    11761 tggagattta tgaccagcac cgcctgacca cctgcgtaca cagcaactgg cagctcaaag
    11821 cactgcaaaa tgcgcggcta aaagcaccgt tggatattta tcttaaagtg aacagtggga
    11881 tgaatcggtt gggcttccag cccgatcgcg tgcttaccgt ctggcagcag ttgcgggcaa
    11941 tggcgaatgt tggcgaaatg accctgatgt cgcattttgc tgaagcggaa catcctgatg
    12001 gaatttccgg cgcgatggcg cgtattgagc aggcggcgga ggggctggag tgtcggcgtt
    12061 cgttgtccaa ttcggcggcg actctgtggc acccggaagc gcattttgac tgggttcggc
    12121 ctggcattat tttgtatggc gcttcgccgt ccggtcagtg gcgtgatatc gccaataccg
    12181 gattacgtcc ggtgatgacg ctaagcagtg agattattgg tgtccagacg ctaaaagcgg
    12241 gcgagcgtgt gggctacggc ggtcgctata ctgcgcgcga tgaacagcga atcggcattg
    12301 tcgccgcagg atacgccgac ggttatccgc gccatgcgcc taccggtact cctgttttag
    12361 tggacggcgt gcgcaccatg acggtgggga ccgtctcgat ggatatgctg gcggttgatt
    12421 taacgccttg cccgcaggcg gggattggta cgccggttga gctgtggggc aaggagatca
    12481 aaattgatga tgtcgccgcc gctgccggaa cggtgggcta tgagttgatg tgcgcgctgg
    12541 cgttacgcgt cccggttgtg acagtgtaac ttgttgtaag ccggatcgga ggcagcgtct
    12601 tctggttgcg aaaaaatcat ccatccggct ggtcagcaac tgtagttgtt aatgtgacag
    12661 agccattgcc catgatagtg tccattaaaa gaatggacac tatttccccg gaacctgaac
    12721 tcgccgcaca ggcgttctac ataaaacgct tacgcttcat tgttgactcg gagtcgactt
    12781 agataaatca cgcttcaccc ttgatgcaga ttcgggttca tgttcagatt gttgattaac
    12841 aggctgcgag tcctgttgtc tttccagact caacaggccc aactgttgat ccaggagctc
    12901 caggtcctcc tgtaccttca cgcgctccag gagttccggg tcatccagga actccagatc
    12961 cataaggtcc tccagctcct cgagtccttc caggtcaacc tagccttcct tgtgcaaaaa
    13021 gtccaaaagg tccttcaggc cctgctgttt tttcaggccc tcgacggctt tgagcagcgt
    13081 cggtaaattg tctttgtccc acgggttacc attacagtca aacaccttca ggctctgggg
    13141 ctgcggcacc tgtaccgttt taaacttgtt ttctccacag tagtaaatta ttagtttggg
    13201 cggcagccgt ggtgaagaga tcaaatcgtt actagaacag tcaaatactt tcaggcgagt
    13261 caagttctcc agcatctcat caggtattga gttaatattg ttgccggcac aggatagttt
    13321 atacaggtac tggggcatcg acggcggtaa aaattgcaag aagttttcag aacagttaat
    13381 cactctcagg ttagtcgaca gctcaggcag ccagataata cagttttcat agcacttcag
    13441 taccatcagt tttgagggct gcaccttcgg taacaagatc aagacgttat ctgtacagtc
    13501 aagcaccagc agccactttg gcatcacctt cggtaacgcg gtcaacacgt ttttactgca
    13561 gtaaaattca atcagatgtg gaggcaaatt ctcagggagt gaagtcaatc ccaggttgct
    13621 aagattgagc atactccgtc ttgtaatgag acattccttc attctgcgca atgcttctgc
    13681 ccggttttca atccggcttg ctggcaggtt ttcttccagg atattttcct tgatttcttc
    13741 cagactttct ttcaggtctt cttggggaat ttctgactgt ttgttttgct ggttttcttc
    13801 caggatttcg tacaggtttt tttgcaggtt ttcttgcaaa ccatcctgct gtatagccca
    13861 ttcgcaccat ttggcgacca tcatgaactc gttatgttgc tgattcaggc gagtccccaa
    13921 cgtgatgatg gtattgttgt ccacctgtat gttcactttg cccgcgatac tgaggtcaat
    13981 gatctgccag ggggcatcac cccagctgaa tttttgcggg ctgagatact tcatgttctg
    14041 caaaatttga ttcttgagtt ccaccgacag gaagagccgc cctggcgggc gtcgtacgtc
    14101 tgggttaacg gaagatgggg gaatggcggg ggcgaatggg cagatgtctt taaagctagc
    14161 cataatgcta cgtttccttg aaataaatat cctctggcag cgccggagac gtgaatgtgg
    14221 tcaccgcaaa ggagccacag gacgtcattt gcgctgatat cactactcat cgttgacttt
    14281 ttccctggtc agaaatgccg tgtcgggtct ggtacatgct gtattacggc ctaggtagct
    14341 ctgacgtatt aaagaaaata cgagagtgaa cgacccggaa ataatcgtta ttaagcaaag
    14401 gaataactat aagaaatcgc tcatgcatcc ggtgctacag gcggatgaaa cgcctattgt
    14461 gtgggctatg tcagtgcggc aggttgcgtg gggaatatgt tcaggcgatg tcaggcaggg
    14521 gcccctgatg gttgaccggt aatgcggggc tggtttttat tacctgttac agtcggaaaa
    14581 agcccttacg ctgactttct tgagccggtg ttttgagttc attagccaga gcctgcaatg
    14641 gtttgtcatc cataatttaa ccttcttctg atgttgggat gagcatatca aaatcaggaa
    14701 atgacaagtt tattacaggc tcaggctgcg ttcagtaaag catcgcatcc ggcgatagat
    14761 gctgcattaa atttaagatt cagcttcctc ttcagccacc cgaacgccaa tcttcaacac
    14821 ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta cctggtcacc
    14881 gacaaccggt gccgcgccta gcaactgctg gacaatttca cccagcgttt gctgtttatc
    14941 acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat atttggcgct
    15001 ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg gcgactggct
    15061 gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca atacatcgcc
    15121 ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa acagggctgc
    15181 aatacgcgtc tcttttggca tatgcaaatc gcgcagtgcc gcacctacac accatttatc
    15241 agcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat ccaggccaac
    15301 gcgtgacacc ggacgtccga cgggcggaac caccactttg gcttttttcg ccgcccacga
    15361 gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga cattaaagaa
    15421 cagtcgcgca ttctccagcc ccgccatcat cgggaacacg gccaggatga ttggcaccgc
    15481 gccgcgtaat cccacccagc tgataaacac gcgctcacgc agattgaaac cgcggaaagg
    15541 gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg ccgacaaaat
    15601 gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc ccagcaccag
    15661 gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga tgccgtagcg
    15721 gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca gaataccgct
    15781 gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg ccagcaatgg
    15841 atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat aaccaccgcc
    15901 aagaccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga acatccaact
    15961 gacactgctt tcatgctgct ggatcattgc aatcagggta atcgtcagaa agaccgccat
    16021 tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt taagcccctt
    16081 accacccagc agagaaaaga ccgctgcagc atcggtggag ccgacgatag cgccgattaa
    16141 taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg ttaaaccaga
    16201 ggtgataagt acgcccagcg tcgccagcga cagcgccggt cctaacgcca cacgaaagga
    16261 actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagcgcca ggttacttac
    16321 catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga ctcccgccag
    16381 catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag aaaatgaact
    16441 aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa ttattgttgt
    16501 ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc taccatatta
    16561 gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt ttaagaacag
    16621 gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg gcatgtgcgc
    16681 ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta atgagtggaa
    16741 tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct gcgtcataat
    16801 cattgggcgt gctgccgcta aagctaccga gaataatcgc cttctgacgt ggcaaaattc
    16861 ccgcatgata gagctgcaac agcatacgtt cgacgcggaa agggtgctcg ttaatatctt
    16921 ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccaatc agtgaaatca
    16981 gcatcgcaag attgcctccc cacaacgtgc cttcggtctg acatgtcggg ccttcccctt
    17041 gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc tcggtaaagg
    17101 cgttcagttc atccgcacca aagtttgcta ccagcatggg gccgctaaag gtgatgacat
    17161 tgccttgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgtccg caaatgagta
    17221 acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc agtaaacgac
    17281 tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtagtc agtctggcaa
    17341 gggaattgag atcttccaga cgctccgttt ccgtacccgc aaaacgctca caacgacggg
    17401 caataacctc gacgttattg acttgatgcc ccgcgtcggt aaggcgctgg ataccacgca
    17461 gcgcggcgtg ctgtttaatg ctgtaacccg atggggcaat taagtgaaac agagacatgg
    17521 caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg tgccgttgtc
    17581 acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat ggtttgcctt
    17641 tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc cgccgtggaa
    17701 cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga aagccggtgc
    17761 agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat cgggtggtaa
    17821 tccgaacgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag cttcaacctc
    17881 cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca gcgagctgaa
    17941 aaatccagag cgtaatattt caatgggggc ggcttacctg aatattctgg aaaccggccc
    18001 gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt catacgctaa
    18061 cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga tcagcaaaat
    18121 caacgatttg gatgctgacg agttcctcga tcacgtagcg cgaaatcatc ctgcgccgca
    18181 ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa tcagtcgcgc
    18241 actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg ctgtaattgc
    18301 cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt ggtgatggtt
    18361 tcattcttgc catcaatcac tttgcgctcg ctggtggaga ttaactgggc gtcaaagtga
    18421 aaaacacccc attgccccat gttgacttca atttgagcga agcgcatacc ttcttgtaat
    18481 tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa atcatacaag
    18541 cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg cggatggagt
    18601 ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg aggcggtacg
    18661 gtaataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac agtaaactcg
    18721 actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag cacggcgatg
    18781 ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat tgccagtatt
    18841 ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc tttgtgcaaa
    18901 tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc atggtaatga
    18961 ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca tagttaacaa
    19021 aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc cgacaaacgc
    19081 ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt tcttgcgacc
    19141 gattcatcga aacgagggcc tgaacatggg aattattgcc tggattattt ttggcctgat
    19201 agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat ttttcctgac
    19261 ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca tgtttggcat
    19321 tggcggctcc atcagcggtt ttaatttgca cagcttccta gtggcggtgg tgggcgctat
    19381 cctggtactg ggcgtattcc gcctcctgcg aagagaataa gattttcata aggcggatag
    19441 cgatacagat gccgctatcc gctttcacat cagaacgtat actcgacacc tgcccagaaa
    19501 ttgcgacctt cttcaataaa cccttcactg taggcatatt gggtatcaaa caggttattg
    19561 accgacgcat tgacgctgaa gccatgacct aaggtgtaat cggctcgaat gtgggtcacc
    19621 gcaaaaccgg cggctttttg tgaaccgtca ctgttgctgt agctggagga acgcgcctct
    19681 tccgacagcg ttacgcttaa cggctcccat ggtttgagtg tcatccatgc ggtcattgtc
    19741 tgcgttggaa gatcggttat cttgccgatg tctttgcgtt tggcgtcagc gtggatcaga
    19801 gcgtagctca atcctacatc cagtatattg ctgattttcc ccttaatacc ggcatccaga
    19861 ccgctgtaat ccaccgtgcc gctgttctga ttttgaatgg tatcggcatt gatattgtgc
    19921 gagaggatgg catcactcac ccggttatag taaacgctga cctcaaatcc ccagtcgcgc
    19981 gtgaaggcac cattccaggt taaatccacc ccgcgagcgc gttccggctt gagctgcggg
    20041 ttaacaatcg ctatctggtt atacgcaggt ttggatgtgg tatagcgttc tttcagcgtc
    20101 ggaaagcgtg tgcggtcata gtacgaaagc gccagagtgt cttcattggc aaagtggtat
    20161 ttccccatca cctgccagtt aaaagctgac tgattgttgt cgtcataatg ggtgatgctg
    20221 ccatctttct cgtgtttttt cgcttctacg ctatcgcgcc agtcatagct gattcccgcc
    20281 acgacatcga cattatcagc agcagcccat tgatattcac tggcgagcga ccaggtacga
    20341 tcttcatagc gatcgtaagc ggcgtgcggc gcaccttttt cccggtgtac gtcatctttc
    20401 cagttaacgg caaacgacag cagatcgttt tcacgcacat cggctgccag ttgtaatccg
    20461 gcaccgtcgc tgtaatcgga ataatggctg tagctgcctt ttttattttt caaatcagcc
    20521 agcgagttgt acatcatcag cgtattttca aaggtgtcgc gatacagccg acttttcagg
    20581 gtaaaacggt cgtttagttg ggtcgctccc tgataataaa aactttcttt gtcatactct
    20641 ggccactgcc agtagcgtga tttttgacca caatttccgc tgtatggcgg gttatctttt
    20701 tcaccatcct gcttaatgta agtcagcgtg tattcatcgt tttcgcgcgg tgtaaaaccg
    20761 agcttcacaa tgccgcgttt atcatcagcc gatgaattaa tcatcttgcc gtgtttgcct
    20821 gcaatatcat tatttacacc atgcggcagg ccgagaaaat cctgctttag ctggctaccg
    20881 ctgacttgca aatacccaag atcgctgctg gcggcaaatg aagcatgcat atcgtaggca
    20941 ttgtcctgac tacggctcca tccctggcga tatcccagac ttgcttcttc agggtggtga
    21001 tattaatggc tccgcccatc tgattaggcc catgaagcag cgacgaatac cctttggaaa
    21061 cttcgactgc ccccagattg ttggtcagaa cccgcgccag atcgaggttg ccgtcatagg
    21121 gaacataaat gggcacaccg tcgaaataga ccggcacctg ccgactatca aagccacgaa
    21181 cttttacctg ttcttcgttg cggctgcctg acttttgcag caccacgccg gggacgacac
    21241 ttaatgcctg agcgacattc tgtttatcca aggctttcat ggtgggttga tcgagaacgg
    21301 tggtcgtcgt cgatgatacc ggactggacc atacggtcag cgtgtcgctc tcctgagccg
    21361 ctacggcctg ctgggtaaag gcaagtgata atgctgtgca gagataacgt tttttgagtc
    21421 tcatagaata aattccgttt aacaaagaat taaatagagg ctgtgggttg cagactgact
    21481 ttaatgtcag ctggtgccgc gtaataaggc gcggaggtaa taaaaaggcg aatgccgcag
    21541 tcgaggtaat ttttgagtgt cgtcagatta attccgccgg tcagagccag cgtgcagtga
    21601 ggagccagcg acggcgctat ttgcgcaatt tcagttgcct gttgcggact aaatttgtcg
    21661 agctgaagta cgtctggttg tgcgcgtaac gcggcgatag cctctttcgg cgtgtcggct
    21721 tcaacaacaa ttttcttctc gggtgcgtgg cgacgtaact gagcgattgc gcctgaccag
    21781 ccctgattgt catgaagaaa atggcggtgg ttggcaaaca ataatatggt ttccgcacat
    21841 ccggcacgat gaattagtcc tccggcagcc agaattgcct gcgaagccag taagcgggtg
    21901 cccggaattg cttttcgggt gcaggcgata ttgccgtcag gataacgttc acgaagcaat
    21961 gccagcattt gagcgagata atcagaaaca ccgcaactcc actccagcac attctggact
    22021 gccttccatc cctgatgaag tgctgcggca ttaccctgcg cacggattag ccgttgaccc
    22081 gcgttcgctt gtgaaccgtc gctgaccgcg tcatcaatag ttaatcccag cgtagttaac
    22141 atcttacacg cgacagaaac gccgctgacg cagccgccct gacggtgaaa aaacgctata
    22201 tagccaggct ggtgtccaat atttaatgct cgcgtggtca ggtcacctcc ctggatatct
    22261 tccagcagta acgcatcgat ttgtgcctga gaaaggaaaa tcatcacagt gcctcctgcg
    22321 gtacgctatc ccaccagatg agcgcccagt cgcggctggc aggggcgagg gcacgagcat
    22381 cttgttgttg ataccagtga tataaccgct cacgctcatc gtcgttaagc gcgccaagac
    22441 tccagctgac gttctgctca aaacgttccc aggtgctgtt atcctgttgg caattttgtc
    22501 cacggataaa atcaacgtgg gcataaattc ccatctgata caggacgttg agcgcaaaga
    22561 tatagttggg taattcgatc acttcccgac cagccgcccg ctgaatggct ggagagacga
    22621 acgaggtgct gaccagatga gttgtataga cgcgaagccg tgcctgatta ttcagtttgc
    22681 tcattgcatg gcgcatatca gccaccagag tggagcggga agccacggca atatcgcagc
    22741 gtggcaggtc actccagtcc tcttcccagg cgcgctggat ccagtggaca ttgtcagcct
    22801 tcagtgcggc ggcgcggcgg gcggcaacgt tcagcatccc ctgactgtaa tcaacgccat
    22861 aaaccgtagt gagtttgtca gccagcgcca gcgatacggt gcctggtccg cagcccatat
    22921 cgaataaggt ctgagcgcct tgcaggtcaa ttttagcgat cagttgttgc aggtagctgt
    22981 ccgttggcga ggcgcaattt tccgccattt tctccgcccg ttgatcccag tgatcgggcg
    23041 ttttttctgt gcggtgagcc agtttgagtt gctgcagata aaggtctgca aaatcaatat
    23101 catcaatgag catgattgtt ccttagcggc taattgcaga gaggtgatga tgaatttggt
    23161 cggcagaaac ccgatacagg gccgccagtt tgttcgttgt taactgttcg gtcggtaagc
    23221 cttgtgttac tcgtccatct ggctctacct ggataatgct gtcggcaatg gcgttggcat
    23281 ggagcggatg gtgagtcgac attaacattg tcatgccgtt atttttgagc tgcgccagcg
    23341 tgtccagaag ttggatctga tgaccgaaat cgaggctgga ggcgggctca tccagtaaca
    23401 acaagcgcgg ttgttggacg agtgcacggg cgattaaagc cagctgacgt tcaccgccgc
    23461 taagcgtatt ccagcgtcgt gtcgccagat gcaaaatgcc cagcttttcc agttgctccg
    23521 tggctttcag gcgttcttgc ttaccgggaa cggaaaaagc gcctatggtg ggggccaggc
    23581 ccatcaacac catatccagc acactgaaag caaaaatacc gtcgtgcgcc tgcggcaccc
    23641 aggcaatagc ctgtgcgcgc tggcgatggc tgagtgtctg cacagggagg ctatccagta
    23701 ggatctcgcc ttttaataac ggtataacgc caagaatgga gcgcatgagt gtggttttgc
    23761 cgcaaccatt cgcgccaagc agacaacaaa ttgttccttg cgacagggtg aaggacacgt
    23821 cgcgtagcac tggatggcgg gagtaaccca gagacgcatg acgaacggca agcaaagtca
    23881 ctggcgacct ccgcgtaaca acaaaagcag gaaaaacggt gcgccaacaa aagcggtcag
    23941 aatgccaagt gggatttctg ttgttccaat gctgcgcgcc agcgtatcgg taagcaggag
    24001 cagaatagcg ccagtacaca ttgccatagg caacagctgc tgatggttat gaccggtcaa
    24061 caagcgggca atgtgcggaa cgaccagacc gatccaacca attatgcccg ctattgctac
    24121 cgtgctggcg gtgattagtg tggcgcagac aatcaatcca aagcgcagac gcgtcacgtt
    24181 cagtcccagc gaacgggctt cgtcgtcgga aagcgtgagt aaattcatgc gccagcggag
    24241 aaaaaacagg ggcaaggagc ctatgagaat gatcggtgca gcatagcaga gatcgcgtaa
    24301 ggtcacggtg gacagtcccc ccagcaacca gaaggtaatt gacggtaact gggtgtaagg
    24361 atcggccagc gttttaattg gcgatatccc cgctccgcac aacgttccta acgcaatgcc
    24421 taccagcacc agggttaaga tcggatcgtg gcgggtcacc cgacgcgtaa tcaaccagac
    24481 gcccgcgacc accatcagct cgccgcaaaa tgcgtacagc tggatgtaaa caatgggtaa
    24541 tccccacagg attgccgcgc aggcaccaag tccggcaccg gcggagacgc ctaaaatatc
    24601 cggtgaaact aagggattac gcaacattcc ctgataggtg gttccggctc ctgccagcgc
    24661 tgcgcccagt aacagcgcag caagaatacg cggcacgcgg atttgccaga agacaatttg
    24721 ttcctgcaca gacacatttt cttgacgaag aattagcgcc agcagttgct gcatatcgag
    24781 atgataagcc ccacttacgg cggcaaaagt gatactgata gctgccagca gcaaaataca
    24841 tccgtttacg attctcatta gctcgccacc aatttttgat agtcggcatc actgagtgaa
    24901 caatgccaga acagttgggc gtaatgctgc atgtcacttt taaactggcg attgatggcg
    24961 ggatccagcc aggcgtgaag tctgcgtaat cccagaagac ggttgattcc cggcggggca
    25021 tcgagccagc caaagggcaa gccacttaaa aataagatgc gttgttccgc aacggctttc
    25081 acgccctgcc agagaggatc acgacgaata aaatctgcag taacggcctc ctgaaccaga
    25141 ataatatccg gctgccagcg caggagattt tccatggaaa cctgtgtcag gccgtggcga
    25201 tcggctattt gcgcgacgtt gtgcaaacct aataattccg ccgcctcggt atgcaacgaa
    25261 ccctgtaagc ccgtttccag ccctcgggga ccgcgcgcag cataaaagcg gaggttagcg
    25321 gcgggtgagg tggcgaatgc ctgagcttca ccaacgaagt ggctggcgag attggcttgt
    25381 tctgcggcgc ggtgctcttc gcctaacgtt ttgccaagcg ttgttaactg ttctgctgat
    25441 tgcgccagtt ttccgttaag caataaccag gggatttgtg tctgttcgct aacctgccgt
    25501 gcctgggaga tcaaggtttc atccgtattg ccgcaatcaa cgaccaaatc gggatgtaac
    25561 gccataagcc cttccagcga tagtgtgctg gcgcgcccgg cgagtcgtcc cagcctgggt
    25621 aactggcgaa tgtgctctgg caatggaatt aatgcctgac gggcaaaatc aaaagaggaa
    25681 aaaccgacca ttttttctgg cgcgacagcc agcaatagca aatcggcggg cgcgcctgcg
    25741 ctgacgatgc gtgtaatgtt ttgggcttcg ggaagagatt gcgaagcaaa acgattaact
    25801 gtttgtgaaa aggacggtag agactgcagg agcagggtag atgcaagtgc ctgcgcaaac
    25861 gtccgtctgg ttatgggcat ggtgaaca
//
LOCUS       DADMGC010000051        25448 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.051, whole genome shotgun sequence.
ACCESSION   DADMGC010000051 DADMGC010000000
VERSION     DADMGC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.051"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            65..523
                     /locus_tag="JNZ73_002036"
     CDS             65..523
                     /locus_tag="JNZ73_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005083197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY8732326.1"
                     /translation="MVNYAFDYLRSPGSLPLTTAATELSAIHGHSTSQYRLGEFYLHG
                     SDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKPDTRKAILWYKEAAEQG
                     YAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(533..1960)
                     /locus_tag="JNZ73_002037"
     CDS             complement(533..1960)
                     /locus_tag="JNZ73_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HAY8732327.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1957..2664)
                     /locus_tag="JNZ73_002038"
     CDS             complement(1957..2664)
                     /locus_tag="JNZ73_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY8732328.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWILSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2822..3805
                     /locus_tag="JNZ73_002039"
     CDS             2822..3805
                     /locus_tag="JNZ73_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HAY8732329.1"
                     /translation="MIIIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAID
                     SPDDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFA
                     SNALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFW
                     YKQAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNA
                     GEGVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECN
                     DATAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(3875..4357)
                     /locus_tag="JNZ73_002040"
     CDS             complement(3875..4357)
                     /locus_tag="JNZ73_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HAY8732330.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            4592..7174
                     /gene="leuS"
                     /locus_tag="JNZ73_002041"
     CDS             4592..7174
                     /gene="leuS"
                     /locus_tag="JNZ73_002041"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAY8732331.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATLEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7189..7770
                     /gene="lptE"
                     /locus_tag="JNZ73_002042"
     CDS             7189..7770
                     /gene="lptE"
                     /locus_tag="JNZ73_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HAY8732332.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            7770..8801
                     /gene="holA"
                     /locus_tag="JNZ73_002043"
     CDS             7770..8801
                     /gene="holA"
                     /locus_tag="JNZ73_002043"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAY8732333.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRTLFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8803..9444
                     /gene="nadD"
                     /locus_tag="JNZ73_002044"
     CDS             8803..9444
                     /gene="nadD"
                     /locus_tag="JNZ73_002044"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAY8732334.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9468..10079
                     /locus_tag="JNZ73_002045"
     CDS             9468..10079
                     /locus_tag="JNZ73_002045"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HAY8732335.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10339..10656
                     /gene="rsfS"
                     /locus_tag="JNZ73_002046"
                     /gene_synonym="rsfA"
     CDS             10339..10656
                     /gene="rsfS"
                     /locus_tag="JNZ73_002046"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAY8732336.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10660..11127
                     /gene="rlmH"
                     /locus_tag="JNZ73_002047"
     CDS             10660..11127
                     /gene="rlmH"
                     /locus_tag="JNZ73_002047"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAY8732337.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVILDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11158..13059
                     /gene="mrdA"
                     /locus_tag="JNZ73_002048"
     CDS             11158..13059
                     /gene="mrdA"
                     /locus_tag="JNZ73_002048"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HAY8732338.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13062..14174
                     /gene="mrdB"
                     /locus_tag="JNZ73_002049"
                     /gene_synonym="rodA"
     CDS             13062..14174
                     /gene="mrdB"
                     /locus_tag="JNZ73_002049"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HAY8732339.1"
                     /translation="MTANPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14185..15273
                     /gene="rlpA"
                     /locus_tag="JNZ73_002050"
     CDS             14185..15273
                     /gene="rlpA"
                     /locus_tag="JNZ73_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HAY8732340.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAQTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15412..16623
                     /gene="dacA"
                     /locus_tag="JNZ73_002051"
     CDS             15412..16623
                     /gene="dacA"
                     /locus_tag="JNZ73_002051"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HAY8732341.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16734..16997
                     /locus_tag="JNZ73_002052"
     CDS             16734..16997
                     /locus_tag="JNZ73_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HAY8732342.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17098..17739
                     /gene="lipB"
                     /locus_tag="JNZ73_002053"
     CDS             17098..17739
                     /gene="lipB"
                     /locus_tag="JNZ73_002053"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HAY8732343.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17998..18951
                     /locus_tag="JNZ73_002054"
     CDS             17998..18951
                     /locus_tag="JNZ73_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAY8732344.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19160..20125
                     /gene="lipA"
                     /locus_tag="JNZ73_002055"
     CDS             19160..20125
                     /gene="lipA"
                     /locus_tag="JNZ73_002055"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HAY8732345.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20226..20429)
                     /gene="tatE"
                     /locus_tag="JNZ73_002056"
     CDS             complement(20226..20429)
                     /gene="tatE"
                     /locus_tag="JNZ73_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HAY8732346.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20558..21346)
                     /locus_tag="JNZ73_002057"
     CDS             complement(20558..21346)
                     /locus_tag="JNZ73_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HAY8732347.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            21439..21822
                     /gene="crcB"
                     /locus_tag="JNZ73_002058"
     CDS             21439..21822
                     /gene="crcB"
                     /locus_tag="JNZ73_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAY8732348.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21876..22085)
                     /gene="cspE"
                     /locus_tag="JNZ73_002059"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21876..22085)
                     /gene="cspE"
                     /locus_tag="JNZ73_002059"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY8732349.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22260..22820)
                     /locus_tag="JNZ73_002060"
     CDS             complement(22260..22820)
                     /locus_tag="JNZ73_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HAY8732350.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23409..24794
                     /gene="dcuC"
                     /locus_tag="JNZ73_002061"
     CDS             23409..24794
                     /gene="dcuC"
                     /locus_tag="JNZ73_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY8732351.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24835..25176)
                     /locus_tag="JNZ73_002062"
                     /pseudo
     CDS             complement(24835..25176)
                     /locus_tag="JNZ73_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT         6516 a         6322 c         6249 g         6361 t
ORIGIN      
        1 ttttatagtt gcagcaatta cctgtattag ccaacccaag aaaacgacgt tgcgtgataa
       61 agccatggtg aattatgcct ttgattattt aagatcacca ggcagtctac cgttaaccac
      121 ggcagccacg gagctttccg cgattcatgg tcactcaaca tcgcaatatc gccttggcga
      181 attttatctt catggtagcg acggtaaacc actggattat acacaggcga gatactggta
      241 tgagcaatca gcagaacagg aaaatccgcg cgcacaaagc aaactggggt ggatctacct
      301 gaaaggcctg ggagtcaaac ccgatacccg taaagcaatt ctctggtata aggaagcagc
      361 tgaacaaggg tatgctcatg ctcaatatac tttgggtttg atctacagaa atggcactgg
      421 tattaatgtt aaccattatg aatctcaaaa atggttaaaa ctggccgcca aacaacatta
      481 caaaaatgcg gaaagattac ttgccggact tcccgcacat taacgtcgac tgtcacccga
      541 agagatggag tattttccct atcaaaccgg caaagataaa aatgtagaaa ataattttga
      601 tgacgcccag acagcccagc ccgccatcgt caacgggcat tggctgcggt tgcggctccg
      661 gctcgacggt ttcctgaccg gtaaaattcg ccgcctgaat taataatcga cgaataatta
      721 atgcttccag tggacaacga tcttctgcat caaggcggct cacaatcggc attaatgatt
      781 cttctgtcgg ttggctggcc tgcactaatt gtttggcaac taaatataat ggccataagg
      841 gatgttctgt atcacgcgtg agtgagatcc gcggcttacg tcctttgtgc aaaaccatct
      901 cgaccagtga acgccccagc gcagagagtt tttcatcgct gagtatttcg tataaatgaa
      961 aacgcgtacg ggcgctgtcg ccggggacat aaagtagcgg ttttccctga tcgttaacca
     1021 gatcacgcgc ttcaatcatc atcaccacta atgggtaata atcgacccga tgctgacggc
     1081 accattgcaa cattaaagca tccgcctggg tggagggata ctcgcgccag tagtaacaaa
     1141 gttctgccag caggctttcg ccttctccac aatagacgcg ttgtgcgtat tcataataac
     1201 gcgcatcttc attatcaggt tcagcttccc gccacgaccg ggcgtaatcc aggagttcgg
     1261 ggattcccag gcggcttgcg ctataccagc ggagtaaatt tctgtgcagt tttgcatcat
     1321 cggggataat cactgcgcga tgttgcgcta aatacaatgt cagccattct ggatgcgatg
     1381 accaaatccg agcgaacatt tggtaaaaat cgatggtgtc attttgcacg gcaacgggca
     1441 gatgcagaat ggtctggaag ttaaaaacat tccccttcgc aatggcatat aaaaaggatt
     1501 ccagttcttc ttcgtctatt tccccgacgt tttcctcctg caaccaccgc aatctttctg
     1561 ctaataacac cacgcactcg aaggataaat gggcagtgtt catggcgatt gtgcacagcg
     1621 accagcgtaa ttcatcaatc tcttccatcg agcaataatt taattgctga atgaatcgct
     1681 gccaggcgga gggcagaaaa cgttcgctat cagaggcaag taacgcgcga aaggcgagca
     1741 gaatttcatg ttctgctacc tcatattctt ctggttgcca aacagattta gcaggcgact
     1801 gcgcaatccg tagcgcctcc tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg
     1861 tttctggatg aaaggacggt aacagcgcca gataagcgcg gcggataata tcgacgtcag
     1921 tcgtttcctc tatatcgagg atcttccagc aatttttcat aagtcgctca tatccttaag
     1981 cgtttccggg catgtggggt agtcagccag tggttgccag gacaaggtag ggtaatacgc
     2041 atcatcactg gcaaataatt gataaaacat atcattgccg gaatcgtcgt attctttacg
     2101 cgctaagaat tcgcgtaata aaggcagatg atcatcttcg ggagactgcc aatattgccg
     2161 accaaaggta taaccggcaa agtattgccg ccagttacta taataacgta gagcccgtag
     2221 atgaatacgc gacaggatcc atagactttc ttcttcactc aaccagttat taagtacgcc
     2281 cattcgactt aaaaaaccca tccggacata atcccatgct ttaattcctc cctcaccaca
     2341 gcattcggct gtgctggcga caaattgcgc ataagcctga ccttgctcat ttaattcagc
     2401 aagatagtca cgccattcac atggagaata acgaaaccag cggtgataaa accctgccaa
     2461 ccgagcggca tgaccgttct cgatcattcg gtgaattgtg cgcagcaggt catcacggtc
     2521 atcaattccc catgaggaag aaaggtcaaa aaagcatgta cctaaataca ttttggggga
     2581 tgaatgcgaa ggtaaacagc attcattgcg tatttccatc ggagtggaaa gtgcaaacaa
     2641 tattttttgc gaccccatgt ccatataatt tatccttaaa cattcattta atgaatgcaa
     2701 actacatttc gagactcagt tatttatact tttctgacgc aacataatac gcataaaata
     2761 aggattacgc aaaaatggaa cacataaaaa tataatatat tttatctaca accataagga
     2821 aatgataata atttttacgt caagttgctg cgataattta tcaatagatg agattatcga
     2881 acgtgctgaa aaaggcgatt gcgaggctca atatattgtt gggttttatt ataatcgcga
     2941 tagcgcaatt gactctccgg acgacgaaaa ggccttttac tggctgaagc tggccgctga
     3001 gcaaggtcat tgtgaagcac agtattcctt agggcaaaag tataccgagg ataaaagccg
     3061 tcataaagat aatgagcaag ccatcttctg gctgaaaaaa gctgccctac aaggccatac
     3121 tttcgcttcc aacgcccttg gctggatact ggatcgtgga gaagacccca actataaaga
     3181 agcggttacc tggtatcaga tagccgcgga gagcggaatg tcttatgcgc aaaataatct
     3241 tggatggatg tacagaaatg gcaacggagt cgcacaagac tatacgctgg catttttctg
     3301 gtacaaacaa gctgcattac aaggccatag ttacgcacaa gacaatctgg ccgatcttta
     3361 taaagacgga gaaggcgttg ctcaaaacaa gacactcgcc gcattctggt atttgaaaag
     3421 cgcacagcag ggtaatcggc acgcccagtt tcaaattgca tgggattata acgctggcga
     3481 aggggtggac caggactata agcaagcgat gtactggtat ctgaaggctg ccgctcagga
     3541 aagcgtcggc gcttacgtca acatcggtta tatgtataaa cacggacaag gcgttgagaa
     3601 agattatcag gctgcctttg aatggtttac gaaagccgct gaatgcaatg acgccactgc
     3661 ctggtataac ctggccatta tgtatcacta cggagaagga agacctgtcg atctccgaca
     3721 ggctctcgac ctgtatcgta aagttcagtc atccggaacc agagatgtca gtcaggaaat
     3781 tcgtgagact gaagctttac tgtagaaata aaaacaaggg ccggggaatt tcattctgcc
     3841 catattctct ttattgctat gcgaaatcag actattacgg ctcaaacggg cgtctctgga
     3901 actggtcata accacatttc gggcatagcg tcagcacttc cggtgtgtag atcgggagat
     3961 ggaagtgaca tttctcgcag accagatttc ccagcccgac cacttctccg ctgtgataaa
     4021 ccccatgatg attgaggtcc tggaaaactt cgcgccattc aagctgcgtt ttatcggtga
     4081 tgtctgccag ctcctgccac aagctttctt taatcacccg cataaagacg ctgtcagatt
     4141 cttctttcag gctctcttca tagctcatgg cgaactcttc caggtcacgt ctgacagctc
     4201 gcgtcagctc atcgatctcg gttcgcgtta actcccctgt ttttattacg cgctcgcgcg
     4261 cctgttccac cagtgcgtcg atatcacgtt cgccattgcg caggcgttcg ttcagtgacg
     4321 cgaccagttc acggtaatat tgagcaacct tgttcatcgt ttcatctccc gggtcgttaa
     4381 ctgactataa taatagacgt tctttgttcc ttgctttgct aatacgccca caaactctgt
     4441 aaattaatgt acgaataatc cgggcatcat ttcaacgtcg agtgcgcgaa aggctgtttt
     4501 gacgcggacg tttgggctat gctatgcgga tctgaaaaac cacctcaacg ctacatttgt
     4561 agccgtattg aaaacaggac cactggctgc catgcaagag caataccgcc cggaagagat
     4621 agaatccaaa gtacagcttc attgggatga gaagcgcaca tttgaagtaa ccgaagacga
     4681 gagcaaagag aagtattact gcctgtctat gcttccctat ccttctggtc gactacacat
     4741 gggccacgta cgtaactaca ccatcggtga cgtgatcgcc cgttaccagc gcatgctggg
     4801 caaaaacgtt ctgcagccga tcggctggga cgcgtttggt ctgcctgcgg aaggcgcggc
     4861 ggtgaaaaac aacaccgctc cggcaccgtg gacgtacgac aacatcgcgt atatgaaaaa
     4921 ccagctcaaa atgctgggct ttggttatga ctggagccgc gagctggcaa cctgtacgcc
     4981 ggaatactac cgttgggaac agaaattctt caccgagctg tataaaaaag gcctggtata
     5041 taaaaagact tctgcggtca actggtgccc gaacgaccag accgtactgg cgaacgaaca
     5101 agttatcgac ggctgctgct ggcgctgcga taccaaagtt gaacgtaaag agatcccgca
     5161 gtggtttatc aaaatcactg cttacgctga cgagctgctc aacgatctgg ataaactgga
     5221 tcactggcca gacaccgtta aaaccatgca gcgtaactgg atcggtcgtt ccgaaggcgt
     5281 ggagatcacc ttcaacgtta acgactatga caacacgctg accgtttaca ctacccgccc
     5341 ggacaccttt atgggttgta cctacctggc ggtagctgcg ggtcatccgc tggcgcagaa
     5401 agcggcggaa aataatcctg aactggcggc ctttattgac gaatgccgta acaccaaagt
     5461 tgccgaagct gaaatggcga cgctggagaa aaaaggcgtc gatactggct ttaaagctgt
     5521 tcacccatta acgggcgaag aaattcccgt ttgggcagca aacttcgtat tgatggagta
     5581 cggcacgggc gcagttatgg cggttccggg tcacgaccag cgcgactacg agtttgcctc
     5641 taaatacggc ctgaacatca aaccggttat cctggcggct gacggctctg agccagatct
     5701 ttctcagcaa gccctgactg aaaaaggcgt gctgttcaac tctggcgagt tcaacggtct
     5761 tgaccatgaa gcggccttca acgccatcgc cgataaactg actgcgatgg gcgttggcga
     5821 gcgtaaagtg aactaccgcc tgcgcgactg gggtgtttct cgtcagcgtt actggggcgc
     5881 gccgattccg atggtgacgc tggaagacgg taccgtaatg ccaaccccgg acgaccagct
     5941 gccggtgatc ctgccggaag atgtggtgat ggacggcatt accagcccga ttaaagcaga
     6001 tccggagtgg gcaaaaacta ccgttaacgg tatgccagcg ctgcgtgaaa ccgacacttt
     6061 cgacaccttt atggagtcct cctggtacta tgcgcgctac acttgcccag agtacaaaga
     6121 aggtatgctg gattccgaag cggctaacta ctggctgccg gtggatatct acattggtgg
     6181 tatcgaacac gccattatgc acctgctcta cttccgcttc ttccacaaac tgatgcgtga
     6241 tgcaggcatg gtgaactctg acgaaccagc gaaacagttg ctgtgtcagg gtatggtgct
     6301 ggcagatgcc ttctactatg ttggcgaaaa cggcgaacgt aactgggttt ccccggttga
     6361 tgctatcgtt gaacgtgacg agaaaggccg tatcgtgaaa gcgaaagatg cggcaggcca
     6421 tgaactggtt tataccggca tgagcaaaat gtccaagtcg aagaacaacg gtatcgaccc
     6481 gcaggtgatg gttgaacgtt acggcgcgga caccgttcgt ctgtttatga tgtttgcttc
     6541 tccggctgat atgactctcg aatggcagga atccggtgtg gaaggggcta accgcttcct
     6601 gaaacgtgtc tggaaactgg tgtacgagca cacagcaaaa ggtgatgttg cggcactgaa
     6661 cgttgatgcg ctgactgaag atcagaaagc gctgcgtcgc gatgtgcata aaactatcgc
     6721 taaagtgacc gatgatatcg gccgtcgtca gaccttcaac accgcaattg cggcgattat
     6781 ggagctgatg aacaaactgg cgaaagcacc gaccgatggc gagcaggatc gcgctctgat
     6841 gcaggaagca ctgctggccg ttgtccgtat gctcaacccg ttcaccccgc acatctgctt
     6901 cacgctgtgg caggaactca aaggcgaagg cgatatcgac aacgcgccgt ggccggttgc
     6961 tgacgaaaaa gcgatggtgg aagactccac gctggtcgtg gtgcaggtta acggtaaagt
     7021 ccgtgccaaa atcaccgttc cggtggacgc aacggaagaa caggttcgcg aacgtgctgg
     7081 ccaggaacat ctggtagcaa aatatcttga tggcgttact gtacgtaaag tgatttacgt
     7141 accaggtaaa ctcctcaatc tggtcgttgg ctaagcgcgg gaggaagcgt gcgatatctg
     7201 gcaacattgt tgttatctct ggcggtgtta atcaccgccg ggtgtggctg gcatctgcgt
     7261 gacaccacgc aggttccttc cactatgaag gtcatgatcc tagactcagg cgatccgaac
     7321 gggccattaa gccgtgcggt gcgtaaccag ttacgtctga atggtgtcga gttgcttgat
     7381 aaagaaacca cgcgtaagga cgttccatcc ctgcgtttgg gtaaagtgag catcgcgaaa
     7441 gataccgcat cggtattccg taacggtcaa acagcagagt atcagatgat catgacggtg
     7501 aatgcgaccg tgttgatccc cggccgtgat atctacccga ttagcgccaa agtcttccgt
     7561 tcattcttcg ataacccgca aatggcgtta gcgaaagata acgaacaaga catgatcgta
     7621 aaagagatgt acgaccgtgc tgccgaacag ctgattcgta agctgccaag catccgtgct
     7681 gcggatattc gttccgacga agaacagacg tcgaccacaa cggatactcc ggcaacgcct
     7741 gcacgcgtct ccaccatgct gggtaactga tgattcggtt gtacccggaa caactccgcg
     7801 cgcagctcaa tgaagggctg cgcgcggcgt atcttttact tggtaacgat cctctgttat
     7861 tgcaggaaag ccaggacgct gttcgccagg tagctgcggc acaaggattc gaagaacacc
     7921 acactttttc cattgatccc aacactgact ggaatgcgat cttttcgtta tgccaggcta
     7981 tgagtctgtt tgccagtcga caaacgctat tgctgttgtt accagaaaac ggaccgaatg
     8041 cggcgatcaa tgaacaactt ctcacactca ccggacttct gcatgacgac ctgctgttga
     8101 tcgtccgcgg taataaatta agcaaagcgc aagaaaatgc cgcctggttt actgctcttg
     8161 cgaatcgcag cgtgcaggtg acctgtcaga caccggagca ggctcagctt ccccgctggg
     8221 ttgctgtgcg cgcaaaacag ctcaacttag aactggatga cgcggcgaat caggtgctct
     8281 gctactgtta tgaaggtaac ctgctggcgc tggctcaggc actggagcgt ttatcgctgc
     8341 tctggccaga cggcaaactg acattaccgc gcgttgaaca ggcggtgaat gatgccgcgc
     8401 atttcacccc ttttcattgg gttgatgctt tgttgatggg aaaaagtaag cgcgcgttgc
     8461 atattcttca gcaactgcgt ctggaaggca gcgagccggt tattttgttg cgcacattac
     8521 aacgtgaact gttgttactg gttaacctga aacgccagtc tgcccatacg ccactgcgta
     8581 cgttgtttga taagcatcgg gtatggcaga accgccgggg catgatgggc gaggcgttaa
     8641 atcgcttaag tcagccgcag ttacgtcagg ccgtgcaact cctgacacga acggaactca
     8701 ccctcaaaca agattacggt cagtcagtgt gggcagagct ggaagggtta tctcttctgt
     8761 tgtgccataa acccctggcg gacgtattta tcgacggttg atatgaaatc tttacaggct
     8821 ctgtttggcg gcacctttga tccggtgcac tatggtcatc taaaacccgt ggaaacgctg
     8881 gcgaatttga ttggtctgac gcgggtcaca atcatcccta ataatgttcc tccgcatcgt
     8941 ccccagccgg aagcgaacag cgtgcagcgt aaacacatgc ttgaactggc gattgccgac
     9001 aagccattat ttactcttga tgaacgcgag ctaaagcgca atgccccctc ttacactgca
     9061 caaacactga aagagtggcg gcaggaacaa ggaccggacg tgccgctggc gtttattatt
     9121 ggtcaggatt cattgctgac ctttccgacc tggtacgaat acgaaacgat actcgacaat
     9181 gcacatttga tcgtctgtcg gcgtccaggt tacccacttg aaatggcgca accgcaatac
     9241 cagcaatggc tggaagatca tttgacacat aacccggaag atcttcacct tcagcctgcc
     9301 ggtaaaattt atctggctga aacgccgtgg tttaacatct cggcgaccat catccgcgaa
     9361 cgtttgcaaa acggtgaatc gtgtgaggat ttattgccgg aaccggtatt gacttacatt
     9421 aaccaacaag gcttgtatcg ctgatacctg tcgttacgga gggcatgatg cgactgtggt
     9481 taattcgtca tggcgaaaca caagcgaatg tcgatggtct gtacagcggt catgcgccca
     9541 cacccctgac cgcacgcggt attgagcaag cgcaaaatct gcatacactg ctagatgatg
     9601 tttcctttga cctggtttta tgcagtgaac tggaacgggc acagcatacc gcgcgactgg
     9661 ttctcagtga ccgccagctc cccgttcata tcatccctga actcaacgaa atgttttttg
     9721 gcgactggga gatgcgacat catcgcgacc tcatgcaaga agatgccgaa aactatagcg
     9781 cgtggtgcaa tgactggcag catgctatcc ccacgaacgg tgaaggattt caggcatttt
     9841 cgcaacgtgt ggaacgcttt atcgcaaggc ttagtgaata tcagcactat cagaatattt
     9901 taatcgtcag ccatcagggc gtactgagtc tgttaattgc tcgtttaatt ggcatgcctg
     9961 ccgagtctat gtggcatttt cgcgttgacc aggggtgttg gagcgccatt gatatcaacc
    10021 aaaaattcgc gacgctacgc gtcctcaata gccgtgccat cggggtcgag aatgcatgac
    10081 ttttctgttt ttttacgggt aagccgcaac gaccattgac agccccgggc aggctgatat
    10141 tctccgcagc cagacttttt ccgccagaca cgactttgta gaaattgttt tacaaaaatg
    10201 gcgatgcaat ctgcggcgcg gggtgggatg atagcccact ttcgaaagcc gattcggcga
    10261 caattgtccc gaaatcgcct ctggttcagg tatactgaca gaccattttt atctatttga
    10321 ttcacccagg gggaaaactt gcagggtaaa gcactccagg attttgttat cgacaaaatt
    10381 gatgacctca aaggtcagga catcatcgcc ttagacgttc aaggcaaatc cagcatcacc
    10441 gactgcatga tcatctgtac gggtacgtcc agccgtcatg ttatgtccat tgctgaccac
    10501 gttgtgcagg agtctcgcgc agcgggcctg ttaccgctcg gcgtagaagg tgaaaacagc
    10561 gccgactgga ttgtcgtgga tttgggcgat gtgattgtcc atgtcatgca ggaagagagc
    10621 cgtcgcctgt atgaactgga aaaactctgg agttaatgcg tgaagctgca acttgtcgcc
    10681 gtgggaacga aaatgccgga ctgggtacaa accggtttta ccgagtacct gcgtcgtttt
    10741 ccgaaagata tgcccttcga gctgattgaa attccggccg gcaaacgcgg caagaatgcg
    10801 gacatcaagc gcatactcga caaagagggt gagcagatgt tggcggccgc aggcaaaaac
    10861 cgcattgtca tcctcgatat tccaggcaag ccctgggata cgccgcagtt agccgctgag
    10921 ctggaacgct ggaagctgga tggtcgcgac gtaagtctac tgattggcgg gcctgaaggg
    10981 ttgtcgcctg cctgtaaagc ggcggctgag cagagctggt cgctgtcggc gcttaccctc
    11041 ccccatccgc tggttcgcgt gctggtcgca gagagtctgt accgggcgtg gagcatcacc
    11101 accaaccatc cttatcaccg tgagtgataa gggagctttg agtagaaaac gcagcggatg
    11161 aaactacaga actcttttcg cgactatacg gcagagtccg cgctgtttgt gcgccgggcg
    11221 ctggtcgcct ttttggggat tttgctgctg accggcgtgc ttatcgccaa cctgtataat
    11281 ctgcaaattg ttcgctttac cgactaccag acccgctcta atgaaaaccg cattaagctg
    11341 gtgcctatcg cgcccagccg cggcattatc tacgaccgta acggtatccc tctggccctc
    11401 aaccgcacta tctaccagat agaaatgatg ccggagaaag tcgataacgt gcagcaaacg
    11461 ctggacgctt tgcgcagcgt ggtagatctg accgatgacg atattgctgc attccgaaaa
    11521 gagcgcgcac gttcacaccg tttcacctct attccggtga aaactaacct gaccgaagta
    11581 caagtagctc gctttgccgt caatcagtac cgttttccgg gtgtcgaagt taaaggctat
    11641 aaacgtcgtt actatcctta cggttcggcg ttgacccacg tcatcggata tgtgtcgaaa
    11701 atcaacgata aagacgtcga acgcctgaat aatgacggca aactggccaa ctatgcggca
    11761 acgcatgata ttggtaagct gggcattgag cgttactatg aagacgtgct gcacggtcag
    11821 accggttatg aagaggttga agttaacaac cgtgggcgtg ttatccgcca gttaaaagaa
    11881 gtaccaccgc aagccggaca cgatatttac ctgacgctgg atctcaaact ccagcaatat
    11941 attgaaacgc tgctggcggg tagccgcgca gctgtggttg ttaccgatcc gcgtacaggt
    12001 ggggtgctgg cgctggtttc cacgcctagt tatgacccaa acctgtttgt tgacggtatc
    12061 tccagcaaag attattccgc cttgctgaac gacccgaata caccgctggt gaaccgcgcc
    12121 acacaggggg tttatcctcc cgcgtctaca gttaaaccct atgtggcggt ttcggcatta
    12181 agcgccgggg tgatcacgcg caatacgacg ctgtttgacc caggctggtg gcaactacca
    12241 gggtcggaaa aacgttatcg tgactggaaa aaatggggcc acgggcgtct gaatgtcaca
    12301 agatcgctgg aagaatctgc ggataccttc ttctatcagg tggcctacga tatggggatc
    12361 gatcgcctct ccgaatggat gggtaaattc ggttatggtc attacaccgg tatcgacctg
    12421 gcggaagaac gttccggcaa catgcctacc cgcgaatgga aacagaaacg ctttaaaaaa
    12481 ccgtggtatc agggtgacac cattccggtt ggtatcggcc agggttactg gacagcgacc
    12541 ccaatccaga tgagtaaggc actgatgatc ctgattaatg acggtatcgt gaaggttccg
    12601 catttgctga tgagcaccgc cgaagacggc aaacaggtgc catgggtaca accgcatgaa
    12661 ccgcccgtcg gcgatattca ttccggttac tgggagctgg cgaaagacgg tatgtacggt
    12721 gttgctaacc gccctaatgg tacggcgcat aaatactttg ccagcgcacc gtacaaaatt
    12781 gcggcgaaat ctggtaccgc tcaggtcttc ggtctgaaag cgaacgaaac ctataatgcg
    12841 cacaaaattg ccgagcgttt acgtgaccac aaactgatga ccgcctttgc gccatacaac
    12901 aatccgcaag tggctgtcgc catgattctg gagaacggcg gcgcgggtcc ggcggttggt
    12961 acgctgatgc gccagatcct cgaccacatt atgctgggtg ataacaacac cgatctgcct
    13021 gcggaaaatc cagcggttgc cgcagcggag gaccattaat catgacggct aatccgaata
    13081 aaaaaacatt ctgggataaa gtccatctcg atcccacaat gctgctgatt ttactggcat
    13141 tgctggttta cagcgccctg gttatctgga gcgccagcgg tcaggatatt ggcatgatgg
    13201 agcgtaaaat cggccaaatc gcgatgggtc tggtcatcat ggtggtgatg gcgcaaattc
    13261 ctccacgcgt ttatgaaggc tgggccccct atctctatat catctgtatt attttgctgg
    13321 tggcggtaga tgctttcgga gccatctcta aaggcgctca acgctggctg gacctcggta
    13381 ttgttcgttt tcagccgtcg gaaattgcca aaatagccgt accactgatg gttgcgcgct
    13441 ttatcaaccg cgacgtttgc ccgccatcgt tgaagaacac tggcatcgcg ctggtgctga
    13501 tatttatgcc cacgctgctg gtggctgcac agcctgacct gggaacatca atcctcgttg
    13561 cgctttccgg tctgtttgta ctgttcctct ctggccttag ctggcgtctg attggcgtcg
    13621 cagtagtgct ggtagcggcg ttcattccga ttctatggtt cttcctgatg catgattacc
    13681 agcgccagcg cgtaatgatg ctcctagacc cggaatcaga cccactcggc gcgggctatc
    13741 acattattca gtctaaaatt gctattggct ccggcggatt acgcggcaaa ggctggctgc
    13801 acggcactca gtcacagctt gaatttctcc ccgaacgcca tactgacttt atcttcgcgg
    13861 tactggcgga agagctggga ttagtgggca ttctgattct gctcgctctc tacattctgc
    13921 tgatcatgcg cgggctgtgg atagccgcca gagcgcaaac cacctttggt cgcgtcatgg
    13981 ctggtggctt aatgctgata ttattcgttt atgtcttcgt aaatattggt atggtaagcg
    14041 gtattctgcc ggttgtaggg gttccgctcc cactggtcag ttatggagga tcggcgctaa
    14101 ttgtgctgat ggctgggttc gggattgtaa tgtcaatcca cacccacagg aaaatgttgt
    14161 cgaaaagcgt gtaagaggtg cgcaatgcgt aagcagtggc tcgggatctg catcgcggca
    14221 ggaatgctcg cggcatgtac aagcgatgat ggtcagcaac agacggtaag tgtaccgcag
    14281 cctgcggtat gtaacggccc catagttgaa attagcgggg cggacccgcg tttcgaacca
    14341 ctgaacgcga cggcaaatca ggattaccag cgcgacggta aaagctacaa aatcgtgcag
    14401 gatccgtctc gatttagcca ggcgggactg gcggcaatct atgatgccga accaggcagt
    14461 aacctgacgg cctctggcga agctttcgat ccgacaaagc tgacggcggc ccatccaacg
    14521 cttccgatcc ccagctacgc cagaatcact aacctggcta acgggcgaat gatcgtggtg
    14581 cgcattaatg atcgcggccc ttacggcaac gaccgcgtta tttcgctttc tcgcgcggca
    14641 gctgaccgtc ttaacacgtc aaacaacacc aaagttcgta tcgatccgat tattgtcgcc
    14701 caggatggtt cgctttctgg tcctggtatg gcttgtacca cagtcgccaa acagacttat
    14761 gccctgcctg cacctcccga tttaagcggt ggcgcgggaa caagttcagt gtctggcccg
    14821 cagggtgaca ttcttccggt cagtaattcg acgctaaaaa gcgaagatcc gaccggcgcg
    14881 ccggtaacca gtagcggttt cctcggcgct caaacgacct tagcgcctgg tgtactggaa
    14941 ggcagcgaac cgacgcctgc gccacagccc gttgttacag cttcgtcgac aacgcctgca
    15001 acctcgcctg caatggtgac accgcaagcc gcctcgcaaa gcgccagcgg caactttatg
    15061 gtgcaagtcg gggccgtaag cgatcaggct cgtgcgcaac agtaccaaca gcaactggga
    15121 cagaagttcg gcgtccccgg tcgcgtaact caaaatggcg cggtctggcg gatccagctt
    15181 ggcccattcg ccagcaaagc cgaagccagt accttgcagc aacgtttgca aaccgaagcc
    15241 caattacagt catttattac taccgcgcag tagcgtaaag caggcatctg atgtgtcaat
    15301 ttctgtaaat gacgttaaca aagtcacgcg gaaagtcaga tgcctgccgg tagtgcattt
    15361 gctatagtag ggcacttttt taattccatc acggatgtcg tagttcagac catgaatacc
    15421 attttttccg ctcgtatcat gaagcgcctg gcgctcacca cggctctttg cacagccttt
    15481 atctctgctg cacatgccga tgacctgaat atcaaaacta tgatcccggg tgtaccgcag
    15541 atcgatgcgg agtcctacat cctgattgac tataactccg gcaaagtgct cgccgaacag
    15601 aacgcagatg tccgccgcga tcctgccagc ctgaccaaaa tgatgaccag ttacgttatc
    15661 ggccaggcaa tgaaagccgg taaatttaaa gaaactgatt tagtcactat cggcaacgac
    15721 gcatgggcca ccggtaaccc ggtgtttaaa ggttcttcgc tgatgttcct caaaccgggc
    15781 atgcaggttc cggtttctca gctgatccgc ggtattaacc tgcaatcggg taacgatgct
    15841 tgtgtcgcca tggccgattt tgccgctggt agccaggacg cttttgttgg cttgatgaac
    15901 agctacgtta acgcactggg cctgaaaaat acccacttcc agacggtaca tggtctggat
    15961 gctgatggtc agtacagctc cgcgcgagat atggcgctga tcggccaggc attgatccgt
    16021 gacgtaccga atgaatactc gatctataaa gaaaaagaat ttacgtttaa cggtattcgc
    16081 cagctgaacc gtaacggcct gttatgggat aacagcctga atgtcgacgg catcaaaacc
    16141 ggacacactg acaaagcagg ttacaacctt gttgcttctg cgactgaagg ccagatgcgc
    16201 ttgatttctg cggtgatggg tggacgtact tttaaaggcc gtgaagccga aagtaaaaaa
    16261 ctgctaacct ggggcttccg tttcttcgaa accgttaacc cactgaaagt aggtaaagag
    16321 ttcgcctcag aaccggtttg gtttagtgat tctgatcgcg cttcgttagg ggttgataaa
    16381 gacgtgtacc tgaccattcc gcgtggtcgc atgaaagatc tgaaagccag ctatgtgctg
    16441 aacagcagtg aattgcatgc gccgctgcaa aagaatcagg tcgtcggaac tatcaacttc
    16501 cagcttgatg gcaaaacgat cgagcaacgc ccgctggttg tgttgcaaga aatcccggaa
    16561 ggtaacttct tcggcaaaat cattgattac attaaattaa tgttccatca ctggtttggt
    16621 taaaaattga acacttgaaa gtgtaatttc cgtccccata tactaagcat cagtaaaaaa
    16681 actcccacct tctggcggga gttgctattt aattacgtta cgccggagct gacatgaaaa
    16741 ccaaacttaa cgaactgctt gaattcccta ctccttttac ttacaaagtt atggggcagg
    16801 cgttacctga gctggttgat caggtggttg aagtggtaca gcgccatgcg ccaggtgact
    16861 acaccccaac ggtaaaacca agcagcaaag gcaactacca ctcggtatct atcactatca
    16921 acgccactca tatcgagcag gttgaaacac tgtatgaaga actgggcaaa atcgatattg
    16981 tccgcatggt tctgtaactc gcttctccgt tacccggctc ctggtcgggt aactctcctc
    17041 tcctcgctgt gatatacttt tcccccactt ttactcattc tccacggaga tgccgttttg
    17101 tatcaggata aaattcttgt ccgccagctc ggtcttcagc cttacgagcc aatctcccgg
    17161 gctatgcatg aattcaccga tacccgcgat aatagtaccc ttgatgaaat ctggctggtc
    17221 gagcactatc cggtattcac ccaaggtcag gcaggaaaag cggagcacat tttaatgccg
    17281 ggtgatattc cggtgatcca gagcgatcgc ggtgggcagg tgacttatca cgggccggga
    17341 caacaggtga tgtatgtgtt gcttaacctg aaacgccgta aactcggtgt gcgtgaactg
    17401 gtgaccttgc ttgagcaaac agtggtgaat accctggctg aactgggtat agaagcgcat
    17461 cctcgggctg acgcgcctgg tgtctatgtc ggggaaaaga aaatttgctc actgggttta
    17521 cgaattcgac gcggttgttc attccacggt ctggcattaa acgtcaatat ggatctttca
    17581 ccatttttac gtattaatcc ttgtgggtat gccggaatgg aaatggctaa aatatcacaa
    17641 tggaaacccg aagcgacgac taataatatt gctccacgtt tactggaaaa tattttagcg
    17701 ctactaaaca atccggactt cgaatatatt accgcttaat tccatacatc aatggcccaa
    17761 tttacactgg gtcattactc ataatacctg ctaatgcttt acatctcctt cgcgtttgta
    17821 tattttcttt atcacttgca gaaatgcggt gccacgcctt gctccgtaac tcaccccatt
    17881 gattaaccta ttgaaatagt tgattatggt gagcaaaagc gttccgttcc aattagacat
    17941 aagattattg taatttttta aatattattt ttccatgaat aaaattggag taagtgcgtg
    18001 gatagtaata atcaaattga accctgttta agcagaaagt cgtcagaagg taaaccacaa
    18061 atatttacaa ccttacgaaa tattgatctt aaccttctga ctatttttga agctgtatat
    18121 gtacataaag ggatcgttaa tgcagcgaaa gtgcttaatc tgaccccctc ggcaatcagt
    18181 cagtctattc agaaactgcg cgttatattc cctgacccat tgtttattcg caaaggccag
    18241 ggtgtcactc ctaccgcatt tgcgatgcat ctacatgagt atatcagtca gggccttgag
    18301 tccattcttg gcgcgctgga tatcgaagga agctatgata agcaacgaac gataactatt
    18361 gcgaccactc cctcggtcgg agccctggtc cttcctgtca tctatcgggc gattaaaact
    18421 cactatccgc agcttttact gcgcaacccg cccatcagcg acgcggaaaa ccaactcagt
    18481 cagtttcaaa ccgatctcat catcgataac atgttttgca ccaatcgtac ggtgcaacat
    18541 catgttctgt tcaccgacaa tatggtgtta atttgccgca agggaaatcc actactctct
    18601 ttagaagatg acagagagac tatcgacaac gctgcgcatg tacttctgtt accggaaggg
    18661 caaaatttca gcggtctgcg gcagagagtt caagagatgt ttccggaccg gcaaatcaat
    18721 ttcaccagct acaacatttt gacaatcgct gcactggttg ccaacagtga catgttagcg
    18781 attattccca gccgttttta taacctgttt agtcgttgct ggccgctgga aaaactgcct
    18841 tttccgtcct taaatgagga gcaaatagat ttctctatcc actacaacaa attcagcctg
    18901 cgtgatccca tcctgcacgg agtaatagat gttatccgta atgcattttg aaaaagtatt
    18961 gttacagcca gatcacagtg cagaacgata aaatggcaca atccaacaac aattttacat
    19021 tttcgcgacc gctttggctg ctttcaggtc cgtttcaatg atatactgcc agtcgttaat
    19081 tcaaaaatag ttgataatta caacaatcta ttgaattgaa acgctttcct tcgtaattcg
    19141 caactggaac acgcacgcta tgagtaaacc cattgtgatg gaacgcggtg ttaaataccg
    19201 cgatgccgat aagatggccc ttatcccggt taaaaacgtg gcaacagagc gcgaagccct
    19261 gctgcgcaag ccggaatgga tgaaaatcaa gcttccagcg gactctacac gtatccaggg
    19321 catcaaagcc gcaatgcgca aaaatggcct gcattctgtc tgcgaggaag cctcctgccc
    19381 taacctggcg gaatgcttca accacggcac agcaacgttt atgatcctcg gcgctatttg
    19441 tacccgccgt tgtccgttct gtgatgttgc ccacggtcgc ccggtagctc ctgatgccaa
    19501 tgaaccagtg aaactggcgc agaccattgc cgatatggcg ctgcgttatg tggttatcac
    19561 ctccgttgac cgtgatgacc tgcgcgatgg cggtgcccaa cactttgcgg attgtattac
    19621 tgccattcgg gaaaaaagcc cgcaaatcaa aattgaaact ctggtgccgg atttccgcgg
    19681 tcgaatggat cgtgctctgg atattctgac tgcaacgccc ccagatgtgt tcaaccataa
    19741 cctggaaaac gtaccgcgta tttaccggca ggtacggcct ggcgcagatt acaactggtc
    19801 gctgaagctg ctggaacgct ttaaagaagc gcatccggaa atcccgacca agtctggtct
    19861 gatggtgggt ctgggtgaaa ccaatgaaga aattattgag gtaatgcgcg acctgcgccg
    19921 tcatggtgtg acgatgttaa cgctggggca atatttgcag ccaagccgcc atcacctgcc
    19981 ggttcaacgt tacgttagcc cggatgagtt cgacgaaatg aaagccgaag cgctggcgat
    20041 gggctttacc catgctgcat gcggtccgtt tgtccgctct tcttaccacg ccgatttgca
    20101 ggcgaaaggg atggaagtta agtaatcctg taataattac ttacatctgt ctgataaaaa
    20161 acccgctttt tgaagcgggt tttttgtatc gaacaagata ttgagggagc gtcctgctcg
    20221 ccacgtcact ctttatgaga gagcttttca gcctgaagat caacgtctgc gccttttttc
    20281 gccgcagcat cgtcatcatt catcgccttc ttgaaccctt taatggccgc tccaaggtct
    20341 ccgcccagcg tacgtaactt cttagtccca aacagcagaa cgaccagcgc cgcaactacc
    20401 agcagtttgg taatactaat ctcacccata gataccttct tgacatataa ccggcaacag
    20461 aacgcgcctt aaaaaccaga gaatattaac ggtcattcga cgcgcgcaca caatagcaat
    20521 ctgtaacaag gtgaatcaag cgccgagtaa aaaaacatca taataattgc ggcggcgcaa
    20581 agcgacggtt gtttaagatg ggcagttgcg cgcgcacctg acgcacacgt tcgggcgtca
    20641 cttccgccat aatgagtgca ggcatttctg acgctgccgc aatggtgacg ccaaagggat
    20701 ctataatccg gctttgcccg atatttttgt tcccgcactc ccccgccgcc accatataac
    20761 aggtggtatc cagcgcacga gcggcaagca acgttgacca gtgatgctct ttgagcggcc
    20821 cacgaaccca ggcggcagga agtaccagga tttcagctcc ctgtaaagcc tgtgccagcg
    20881 ccagctctgg aaagcgtaag tcataacagg tcatcagacc gaccttcatc ccttccacct
    20941 ccagtaacgg agctatttca ttaccagcat caacatggcg tgattcctga atggcaaatg
    21001 catcatagag atgcagtttg gcataacggg cgacgatgtt tcctgcctga agtgccacca
    21061 gcatattcca tgcgcgcccc ggcgttgaag gaagatgaat cgtcagaatt gtcgtcatca
    21121 tgttacgttt actttctcgc cgtaaacgtc cgaggaattc gccttccagc agctgtgctg
    21181 atttaaccga tagatctgca tcatgatcat cgcgcgccag caatgcttcc ggcaggacaa
    21241 acagcgatac gtcgttttcc gccgcctgcg ccatcaacga ggcacaaatc tcagcgttct
    21301 tttcccacac agatgtaaca gcaaactgtc ctgcagcaac cagcattgcc acctcctgtc
    21361 agagtcgtaa aattcgccaa cgacgattat tcggcgttac acttatcact catacaaatc
    21421 aaatagcagg attttgcagt gttacaactt cttttagcag tttttattgg cggtggtacg
    21481 ggaagcgtag cgagatggct gttaagtatg cgatttaacc cgctgcatca ggcgattccg
    21541 ttggggacgc tggcagcaaa cctgattggg gcattcatca taggaatggg attcgcctgg
    21601 ttcagcagga tgacgaacat tgatccagtg tggaaagtat taatcaccac cggattttgt
    21661 ggcggtctaa caaccttctc aacattttcg gcagaagtgg tgtttttgtt acaagagggc
    21721 cgctttggct gggcattact gaacgttttc gtcaaccttc tggggtcttt tgccatgacc
    21781 gcactggcat tctggctgtt ttcggcctca accgcacact aaagaccaaa aaaaacccgc
    21841 tgattaagcg ggttttgaat tcttgctgac gtatcttaca gagcgattac gtttgcagca
    21901 gaagggcctt tggcaccgtt agtgatttcg aactctacgc gctgaccttc agcaagagtt
    21961 ttaaaaccat tagtctggat tgcagagaag tgtacgaaca cgtctttgct gccgtcttcc
    22021 ggagtaatga aaccgaatcc tttggactca ttaaaccact taacgttacc tttaatctta
    22081 gacatcaaaa ttacctttac atgaaaaata gacacaaatg ctgtgtcggt tatcagtaca
    22141 ccaattgtgg tacgctttgt ccagtgcaac tttactaaaa agtgataaaa gtcgcgttat
    22201 ttttgatgtt gcttatttta gttattgatt ttaaagaagt tactaaaact tcatttgcct
    22261 caaaactgaa agcgcatcca ggcaaagtac acattgccat tgttgtaggt acccggaatg
    22321 taggtcatct gaaaagtcac tgggccataa cccacggagg ccaatggcag tagaaccggg
    22381 agagggatgt aattccagtt atcgcgtgcc gttacgccag cggtgaatcc cagacctaaa
    22441 tgaaaatttt catccgccag cggtcgccag gtactttccc atccgtatcc ggcaatcggt
    22501 tcccatttgt tccacgagtc cttaaatgcc atggcataca ggccatgcca gtttcctttt
    22561 tcatcccaac gcgacaggcc aaaaccgcca ccccacggtc gctcgttata gcgatcggtt
    22621 ttttctttgt cgtaagcgaa acgtgcatgc caggtgatgg caggaatata taaatcataa
    22681 tgttcaggct gttgccaggt ttgtgcaata ttttctctaa acgttgtcat cctctcatct
    22741 gcgttagcaa aaactttacc aacgctgatt aactgaataa aaacaaagga aaagatagcg
    22801 acatatttac tcacgttcat ttgtgaccat aaaacattta tcaaaaatct actactagca
    22861 tagcaaagct acaattaaca taaccttaat agacccaaca taaagaataa tctgaatagc
    22921 tgctctattt atttccgctt gttcaagtta aatatttccg cgcatcgatt aaagatgatg
    22981 cgtcgttttc tgcgatggga atagtcaaaa aagaaaaacc aagtcttttt tgatgacaaa
    23041 atgcaatcaa ggaaaaatta ttttattttt ttaacgagat acaacaatca tcttaacgaa
    23101 gtatataata tatatcgtca acccatgctc atattagatt attttttgag cgcacagccg
    23161 cagctaacac cattgcaatt aacaaatttg catcaatcca ccatcaattt acacacatta
    23221 ttatgtgata attgccaacc gctaaatatg cgttttgtta tctatgtata aaaacagcaa
    23281 cttcaatgtc ttaatggcag tttttcttga ttttaatcag cattcatcgc caatttattg
    23341 ggcatatttt ttccttaagc tttaggaagt ttttattatt tactttgggc ctggagacag
    23401 gaaaaattat gctgacattc attgaactcc ttattggggt tgtggttatt gtgggtgtag
    23461 ctcgctacat cattaaaggg tattccgcca ctggtgtgtt atttgtcggt ggcctgttat
    23521 tgctgattat cagtgccatt atggggcaca aagtgttacc gtccagccag gcttcaacag
    23581 gctacagcgc cacggatatc gttgaatacg ttaaaatatt actaatgagc cgcggcggcg
    23641 acctcggcat gatgattatg atgctgtgtg gctttgccgc ttacatgacc catatcggcg
    23701 cgaatgatat ggtggtcaag ctggcgtcaa aaccattgca gtatattaac tccccttacc
    23761 tgctgatgat tgccgcctat tttgttgctt gtctgatgtc actggccgtc tcttccgcaa
    23821 ccggtctggg tgttttgctg atggcaaccc tgtttccggt gatggtaaac gttggtatca
    23881 gtcgtggcgc agcagctgcc atttgtgcct ccccggcggc gattattctc gcaccgactt
    23941 caggggatgt ggtgctggcg gcgcaggctt ccgaaatgtc gctgattgac ttcgccttca
    24001 aaacgacgct gcctatctca attgctgcaa ttatcggcat ggcgatcgcc cacttcttct
    24061 ggcaacgtta tctggataaa aaagagcaca tctctcatga aatgttagat gtcagtgaaa
    24121 tcaccaccac tgcccccgcg ttttatgcca ttttgccgtt cacgccgatc atcggtgtac
    24181 tgatttttga cggtaaatgg ggtccgcaat tacacatcat cactattctg gtgatttgta
    24241 tgctgattgc ctccattctg gagttcctcc gcagctttaa tacccagaaa gttttctctg
    24301 gtctggaagt ggcttatcgc gggatggcag atgcgtttgc taacgtggtg atgctgctgg
    24361 ttgccgctgg ggtattcgct caggggctta gcaccatcgg ctttattcaa agtctgattt
    24421 ctatcgctac ctcgtttggt tcggcgagta tcatcctgat gctggtattg gtgattctga
    24481 ccatgctggc ggcagtcacg accggttcag gcaatgcgcc gttttatgcg tttgttgaga
    24541 tgatcccgaa actggcgcac tcttccggca ttaacccggc gtatttgact atcccgatgc
    24601 tgcaggcgtc aaacctgggt cgtaccctat cacccgtttc tggcgtagtc gttgcggttg
    24661 ccgggatggc gaaaatctca ccatttgaag tcgtaaaacg cacctcggtg ccggtgcttg
    24721 ttgggctagt gattgttatc gttgctacag agctgatggt gccaggaacg gcagcagcgg
    24781 tcacaggcaa gtaaatagta atgccggagg caagtttcct ccggctttat catgtcaccc
    24841 actgtggtat atgcgttgtg gtctgccaac tttgccgtga acaatttcag caataatcag
    24901 atggcggctg gcgcaatatt caagataacg cctggcagtg gtgcggctga tggtcagtgc
    24961 ctgcgccacc gtttctgccg tatgttgcac accaggctct ttaaacagtt ttcgcaccgc
    25021 gtttagcgtc aaggggtcaa tgccggtcgg cagttcgtct ttgggttcac cgcgagcata
    25081 ggcattaaac atctcatcaa tttgcttctg gctggcgcta tcaatacttt ccagcatatg
    25141 tttacactgg cggaaacggg ttagcgtttg ccccagtgta ttgattcact tgaagtacga
    25201 aaaaaccggg agaacattgg attattcggg atctgatggg attagatttg gtggggcttg
    25261 caagcctgta gtacaaattt taggcgttaa ccaatgaaac gcgaaagata gtaaaaaatt
    25321 gcttttgttt cattgaaaaa acgaaaaaca aaaacactat aaatcatttc aatcatagct
    25381 tcaaaaaacg ttcaaaaccg ataacaacca ggctgtcacc aaatgactca tatcacaaat
    25441 cagctcat
//
LOCUS       DADMGC010000052        24860 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.052, whole genome shotgun sequence.
ACCESSION   DADMGC010000052 DADMGC010000000
VERSION     DADMGC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.052"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            72..353
                     /locus_tag="JNZ73_002063"
     CDS             72..353
                     /locus_tag="JNZ73_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY8732352.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            319..1396
                     /locus_tag="JNZ73_002064"
                     /pseudo
     CDS             319..1396
                     /locus_tag="JNZ73_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1789..2130)
                     /locus_tag="JNZ73_002065"
     CDS             complement(1789..2130)
                     /locus_tag="JNZ73_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HAY8732353.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2143..3015)
                     /gene="copD"
                     /locus_tag="JNZ73_002066"
                     /pseudo
     CDS             complement(2143..3015)
                     /gene="copD"
                     /locus_tag="JNZ73_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3019..3393)
                     /gene="yobA"
                     /locus_tag="JNZ73_002067"
     CDS             complement(3019..3393)
                     /gene="yobA"
                     /locus_tag="JNZ73_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HAY8732354.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3532..3762
                     /locus_tag="JNZ73_002068"
     CDS             3532..3762
                     /locus_tag="JNZ73_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAY8732355.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3864..4520
                     /locus_tag="JNZ73_002069"
     CDS             3864..4520
                     /locus_tag="JNZ73_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAY8732356.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGNYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4544..5206
                     /gene="exoX"
                     /locus_tag="JNZ73_002070"
     CDS             4544..5206
                     /gene="exoX"
                     /locus_tag="JNZ73_002070"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HAY8732357.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5203..7263)
                     /gene="ptrB"
                     /locus_tag="JNZ73_002071"
     CDS             complement(5203..7263)
                     /gene="ptrB"
                     /locus_tag="JNZ73_002071"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HAY8732358.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPT
                     TPAD"
     gene            complement(7472..8131)
                     /locus_tag="JNZ73_002072"
     CDS             complement(7472..8131)
                     /locus_tag="JNZ73_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HAY8732359.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8458..8814)
                     /gene="yebF"
                     /locus_tag="JNZ73_002073"
     CDS             complement(8458..8814)
                     /gene="yebF"
                     /locus_tag="JNZ73_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HAY8732360.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDATGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8881..9171)
                     /gene="yebG"
                     /locus_tag="JNZ73_002074"
                     /pseudo
     CDS             complement(8881..9171)
                     /gene="yebG"
                     /locus_tag="JNZ73_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9305..10483
                     /gene="purT"
                     /locus_tag="JNZ73_002075"
     CDS             9305..10483
                     /gene="purT"
                     /locus_tag="JNZ73_002075"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HAY8732361.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10538..11179)
                     /locus_tag="JNZ73_002076"
     CDS             complement(10538..11179)
                     /locus_tag="JNZ73_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAY8732362.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11216..13027)
                     /gene="edd"
                     /locus_tag="JNZ73_002077"
     CDS             complement(11216..13027)
                     /gene="edd"
                     /locus_tag="JNZ73_002077"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAY8732363.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLFRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVRTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13262..14737)
                     /gene="zwf"
                     /locus_tag="JNZ73_002078"
     CDS             complement(13262..14737)
                     /gene="zwf"
                     /locus_tag="JNZ73_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAY8732364.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15075..15944
                     /locus_tag="JNZ73_002079"
     CDS             15075..15944
                     /locus_tag="JNZ73_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY8732365.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16072..17514
                     /gene="pyk"
                     /locus_tag="JNZ73_002080"
     CDS             16072..17514
                     /gene="pyk"
                     /locus_tag="JNZ73_002080"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAY8732366.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMHADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17645..18616)
                     /gene="lpxM"
                     /locus_tag="JNZ73_002081"
                     /gene_synonym="msbB"
     CDS             complement(17645..18616)
                     /gene="lpxM"
                     /locus_tag="JNZ73_002081"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY8732367.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18736..20058)
                     /gene="mepM"
                     /locus_tag="JNZ73_002082"
     CDS             complement(18736..20058)
                     /gene="mepM"
                     /locus_tag="JNZ73_002082"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HAY8732368.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLLFD"
     gene            complement(20074..21006)
                     /gene="znuA"
                     /locus_tag="JNZ73_002083"
     CDS             complement(20074..21006)
                     /gene="znuA"
                     /locus_tag="JNZ73_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HAY8732369.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21085..21840
                     /gene="znuC"
                     /locus_tag="JNZ73_002084"
     CDS             21085..21840
                     /gene="znuC"
                     /locus_tag="JNZ73_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HAY8732370.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21837..22622
                     /gene="znuB"
                     /locus_tag="JNZ73_002085"
     CDS             21837..22622
                     /gene="znuB"
                     /locus_tag="JNZ73_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HAY8732371.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22769..23779)
                     /gene="ruvB"
                     /locus_tag="JNZ73_002086"
     CDS             complement(22769..23779)
                     /gene="ruvB"
                     /locus_tag="JNZ73_002086"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAY8732372.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23788..24399)
                     /gene="ruvA"
                     /locus_tag="JNZ73_002087"
     CDS             complement(23788..24399)
                     /gene="ruvA"
                     /locus_tag="JNZ73_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAY8732373.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(24538..24603)
                     /locus_tag="JNZ73_002088"
     CDS             complement(24538..24603)
                     /locus_tag="JNZ73_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732374.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24674..>24860
                     /locus_tag="JNZ73_002089"
     CDS             24674..>24860
                     /locus_tag="JNZ73_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732375.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTIT"
BASE COUNT         6170 a         6293 c         6104 g         6293 t
ORIGIN      
        1 ttattaaaaa tcaaccacac ccgccagtat tctgtatatt tactggcggt catatcgtga
       61 gaggtatggc aatgaatctt gtgacactca aaacgtgggg aaaactcaga tatccggata
      121 acccaccatc aatatcaacg ctgagacgat gggcaaggaa tggaaacatt tatcctgcac
      181 ctgaactaca cgggaggagt tacagggtgg ttccggaggc tttctatatc aacccaaata
      241 aggttgatac cgatataaca caccatcagc ctaatgggcg acaagggaga gacagtccgt
      301 taatggagaa gttaaaacat gcagcggaaa aaatacgatc ccaatttgcc tagaaactta
      361 acatatcgaa ggagagacaa agcatattac tggcgcaacc ctctgacgaa agaagaattt
      421 acactaggta aaatttcaag aagagatgca gtagcgcagg caattgaagc aaatcattat
      481 atatacaaaa actactctcc tgctgcccta attgaaaagc ttaaaggggt cgactcattt
      541 actatggcag actggattga acgttacaaa acgattctta taaggagaaa agcgtccaga
      601 aatacttata aaactcgggt aaatcaactg gtgacaataa aagaaaaatt aggaggggtt
      661 ttactgacag aaataaccac tcgccatatc gccgagtttc ttgatttgtg gattgaagga
      721 gggaaaaaca cgatggcagg atcaatgcgt tctgtgttat ctgatatgtt ccgcgaagcc
      781 attgttgaag gacgtatatc tcaaaatcca gtaacgccaa caagagcacc gaaaatagta
      841 gttacaagag aacggctgaa actaaagaca tacaactgca tcagggaggc agcagatcaa
      901 cttccggcat ggttcccatt agctatggac ttagcccttg taacaggaca acgtcgcgaa
      961 gacataacga atatgcggtt cagtgatatt tatgatgatc gtctccacat caggcaaatt
     1021 aagacaggaa tgatgattgc tatccccctg tcactcagcc ttcctgtcgc tggtttacga
     1081 cttggtgcag tagttgaaca gtgccgcatg gtaagtaggg ggggattatc taatcagtgc
     1141 cgggattaga aaaaacagcc ctgacggcag cattcacccg gacggcctga caaagaaatt
     1201 tgtcgcagcc agaaaattaa caggtatcct gttcagtgaa aacccaccaa cttttcacga
     1261 aatcagaagc ctggctggac gattgtacaa agaaatatgt ggagaagaat ttgctcagcg
     1321 tctacttggc cacacatcgg agaagacaac aaaaatgtat cttgatgaga gagaaaaaac
     1381 gtacttactg ctctgatttt aacgtaaatg gattgttaaa tgtattttgg ttgtgatata
     1441 accaaaaaag accggaatac agaaattcga gtaaatttcg gggaatttcg gggagacgtt
     1501 tgcaactaat tgattttaaa tacaattaaa aaaagaccga atacgattcc tgtattcggt
     1561 ccagggaaat ggctcttggg agagagccgt gcgctaaaag ttggcattaa tgcaggctta
     1621 gttgccttgc cctttaagaa tagatgacga cgccaggttt tccagtttgc gtgcaaaatg
     1681 gtcaataaaa agcgcggtgg tcatcagctt aaatgttaaa aaccgcccgt tctggtgaaa
     1741 gaactgaggc ggttttttta ttggaaatca aaaggctatt ttaggtaatt aacagagttt
     1801 ttcagctcgt tctataaacg gtgccagact cattttttcg ccgggattgt taggatcatc
     1861 aatctgaatc accgaaatgg gttgggcatt ggtcttccca ctggcaactt ccttttgtgc
     1921 gatatcgttt aaaggatact gcacgagggt acttgggttg atgacataca aagcattacc
     1981 cggtcggcaa gtcagcatta cctcttcgcg attaaacgcc catttgtctt tacccacttc
     2041 aaaacgactc acggttatca cctgtggagc cgccagcgcc gctgcagaac tggtgagtaa
     2101 cagaaacgcc agaatacttt ttttcatcat tttcgtaatc catcaaaaag gttcgagagt
     2161 cgcaagcagg ctgatgatca ccagcactac cgcacctatc gcccattcga gtttagtcat
     2221 ccacacaaac cagggtgccg cacgatcttc atcctgcctc atgcgcggta caagaacata
     2281 acgattagcc agcgcaatga ccaccataat catcaccagg atggctttga gcaataagag
     2341 ttggccctag taagtcgtaa gcgtgggggg aaatccggta atcaacaatg cattaagcac
     2401 gccgcttgcc agtacgccga tcaccgcaaa atgcccgcac caggaaaaac gcatcagcgc
     2461 ctgaatagcc tggtgtcgcc aacggccttt gatgagttgc atacaccaga gcaccggcag
     2521 taatccccca aaccacgccg ccgcacaaat caggtgaatc gcgtgattag tctgatggat
     2581 tttcgctgtt accccttcat tcagcgtcgc atgccccact cccgccagca gaataaattg
     2641 cgcggtagtg agcataaaca acaaacgtgg catattccgc ggttgcataa gggcgacgat
     2701 caacgttacg agggcgagaa caatttgcca taaccagacg ccaccaaact gcgtttgcaa
     2761 aacagcctgc caaatgtttg gcgaaaatac atctgtccat cccgttccca tcaacccgcc
     2821 ctgaacggcg agcattgcag tggcactgat caaactccag acggcagcgt gctgttgtaa
     2881 acgcagagaa cgcttcgtca gcaagcgacg aatcattaag ggggccagcc aggcaccata
     2941 cattgcaaag ccaaagacca gcatcaacga ggtgaaatgg ataaatcgca gcgcgatcca
     3001 ggtaaacgcc agcatgattt atttcacgct aaaggtgtag tgtcctttcg ttttatgccc
     3061 gtccaccgaa acaacatgcc agtcaacggt gtaggttccg ggtttcagcg aatcggccag
     3121 tggaacaatc agttgctttt ggtcctgctc attccgcttc gctggtaatg ttttaatatt
     3181 ttcgtttttt ggccccgtga tttttgcacc actgaatcct gtttcaacgc cttccgagaa
     3241 gtttaaagtg attgcctgcg gggcagctgt cacttgcgcg tttgccgcag gatactgatg
     3301 cgttaaatgc gcatgtgccc agacggaagg tgtcaccaac gaagtggtca ggattgccag
     3361 cgcgtagcga agggagcgtg cagttgaagc catattatct attccttttt gtaataactt
     3421 ttttacagag cataaccttg tctaatgtct gagtcgagga tcatcaattc cggcttgcca
     3481 tccaggctca ctcttagtaa cttttgcccg cgaatgatga ggagattaag aatgctgaag
     3541 aatctggcta aactggatca aacagaaatg gataaagtga atgtcgattt ggcggcggcc
     3601 ggggtggctt ttaaagaacg ctacaatatg ccggtgatcg ctgaagcggt tgaacgtgaa
     3661 cagccagaac atttgcgcag ctggtttcgc gagcggctta ttgcccaccg tttggcttcg
     3721 gtcaatctgt cacgtttacc ttacgagccc aaacttaaat aaaacttata cagagttaca
     3781 ctttcttaca taacgcctgc taaattatga gtattttcta aaccgccccc ataatttgca
     3841 gtcattttga aaaggaagtc attatgtcgt tctggaaagt tgcagcagcg caatatgagc
     3901 ctcgtaaaac ctcactcacc gaacaggtag ctcatcatct ggagtttgtg agggccgccg
     3961 ccaggcaaca atgtcagctg ttagtttttc catcgctttc tttactgggg tgtgattatt
     4021 cgcgacgtgc ccttcctgcc ccacccgatc tttcactgtt agacccgctt tgttatgccg
     4081 caacgacctg gcgaatgacc atcattgccg gccttcccgt tgaatataac gatcgcttta
     4141 ttcgtggaat tgcggtgttc gcaccgtggc gaaaaacgcc tgggaactac catcagagtc
     4201 atggtgcatg tctgggccgt cgttccagaa ccatcacggt ggtcgatgaa caaccgcaag
     4261 gtatggatat ggacccgacc tgttcactgt ttacgacagg gcaatgtctg ggagaacccg
     4321 acctgctggc gtcggcccgt cgcttacaat ttttttcaca tcagtactcg attgccgtgc
     4381 tgatggcaaa tgcccgtggt aacagtgcac tatgggatga atatggtcgt ctcatcgttc
     4441 gcgccgatcg tggttcatta ttgttagtcg gtcagcgttc atcacagggt tggcaaggcg
     4501 atatcattcc attacgctag gctttttcgg cctggagcat gccatgttgc gcattatcga
     4561 tacagaaacc tgcggtttgc agggagggat cgttgagatt gcctctgttg atgtcattga
     4621 cggaaaaatc gtcaacccca tgagcctcct ggtgcgcccc gatcgtccta ttagtccaca
     4681 agcgatggcg attcatcgca tcaccgaagc catggtcgcc gataaaccgt ggattgaaga
     4741 tgtgatccca cactattacg gtagtgaatg gtatgtcgcg cacaacgcca gctttgaccg
     4801 ccgcgtactg cctgagatgc ccggtgagtg gatttgcact atgaaactgg cccgtcgttt
     4861 gtggccaggg ctcaagtaca gcaatatggc gttatataaa acacgcaagc tcaatgtaca
     4921 gacaccgccg ggcctgcatc atcaccgcgc gttgtatgac tgttatatca ccgccgcgtt
     4981 gcttatcgat attatgaaca cctccggctg gacggcagaa cagatggccg atatcaccgg
     5041 acgtccgtcg ttgatgacga ccttcacctt tggcaaatac cgtggcaaag cggtttccga
     5101 cgttgccgaa cgcgatccgg gctatctgcg ctggttattt aataacctgg acagcatgag
     5161 cccggagctg cgtttaacac tgaaacatta tctggaaaat acttaatccg caggcgtagt
     5221 gggtaatgtt ccctgcgcca gcgcgaccag aaaagcatat tccatcgcta cgccttcgta
     5281 cgatttaaag cggccagatt taccgccatg gcctgagtcc atgtcggtac agagcaataa
     5341 aagatggtca tcggttttca gctcgcgcaa tttagcgacc cattttgccg gttcccaata
     5401 ttgcacctga gaatcgtgta aaccggtcgt taccagtaaa tgcggataag cctgtgcggt
     5461 gacgttgtca tacgggctgt agcttttcat gtactcgtaa tattgcggat cctgcggatt
     5521 tccccactct tcaaactcac cagtggtaag aggaattgat tcatcaagca tcgttgttac
     5581 aacatcaaca aacggtacct gggcgataac gccgtggaat aattccgggc gttgattaat
     5641 tgcaacgccc atcaacatgc cccccgcact tccgcccatc gcataacaga gcgaaggaga
     5701 gccatagccc agttttaaca atgcatcgca ggcatcaaga taatcattaa acgtattttt
     5761 cttcttcaga aatttaccgt cttcgtacca ttgttgcccc agctcaccac cgccgcgaac
     5821 atggacaatg gcgtagacaa agccacgatc taacaaactc aagcggctaa aactgaaatc
     5881 ggcatcaata cttgcgccgt aagaaccata gccatacacc agcaacgggt tgtgtccttt
     5941 gcgaaaatgt ttgcgatgat agaccagcga aaccggaact tcgacgccat cacgggcgac
     6001 tatccacagg tgttcactgc ggtaattcgc cgcatcaaaa ccgggcactt ctgtttgttt
     6061 taatacacga cgctcaccgg tatccatatc cagttcaaac aaagtgtctg gtgtggtcat
     6121 ggaagaataa ccataacgca atcgcgcggt ttcaggttct ggattgtagg caatccaggt
     6181 cacataggcc ggatcatcaa aggcaatgcc aatgacttcc cgggtcttgc ggttaatttg
     6241 gcgcaaactg gttaacccgc gctgacgctc ttcaaccacc agccagtcgg taaacagcgt
     6301 aaacccttcc agcatgatgt tttcacgtgg cggaattaac tcttcccact gttgctcatc
     6361 acgcatacgg gtacggtata agccaaagtt tttgccgtgg cgattggaac gcagataaaa
     6421 acgatgctgg tagtgatcaa ggctgtattc gtgatctttg cggcgcggca gaaaaacaaa
     6481 cggctcggca tcggccattt ccgcgtccag caggcgaact tcactggtgg tggcgctggc
     6541 cagatgaatg acgacatagt gcttcgacgt cgttttatgc aggctgacat aataggtatc
     6601 gtctttttct tcgtagatca gtgtatcttg cgatgctggc gtaccgatgg cgtgacgcca
     6661 gacctgataa ggcagcagcg tcaccggatg cttgcgaaca tagtagaaag tccaggagtc
     6721 atttgcccag acaaagctgg gttcaacgtt atccagcagt tccgggtacc agttacctgt
     6781 ttccagatta cgaaaacgaa tgccgtactg gcgtcgggaa agaaaatctt ctgccagcgc
     6841 cataatggtg ttatcgggcg taatcgccat tccgcccatc gaataaaact cactatgagc
     6901 tgcgcgcttg ttggcatcga gcaatgtttc ccactcatcc cactcttcgc tgaatgccga
     6961 ttgacgctgg tagatagcat attcacagcc tggttcataa atatgccgat agcggtagcc
     7021 attttttatg tagggcgcag aaacttctct ttgcggaatg cggtcgatga tttcctttaa
     7081 gatgcgatcc tgcaaggctt gttgtgaggc catcacccga tgaccgtaac tattttcttg
     7141 ttgcaggtag tccaggactt ctggctgaga acgcgtatcg tcccgcagcc agtagtaatt
     7201 atcgatgcgc gtatcgccat gaagcgtcat ggcgtgggga atgcgggcgg cttttggtag
     7261 catgttattg ttctttctgg ttgaaacatc ttataagggt ggcaaaactc accggggatg
     7321 caagcgaaac agggtcgtca ttgcttagat gatgacaggt aatggcgcgg atatcgaatg
     7381 ttatgcagac agagaaaatc agcctgttca aatggctgtg cggttctgga tagcccgaaa
     7441 tagtcaactt caggctatcc agagagcgga attattccgc caaagtgcgt ttttgctgtt
     7501 cgagatcgcg ttcaatgcct tcacgaacat cctgggggat tttcagcgcg tcacccagtg
     7561 cattcaggta actgcgttcc ataaaatggt caatatcaat agccgcgcag ctcaggaaat
     7621 agatttccag cgcctcttct tcattgcgga ccccggtagc caggcgttgt ggatccagcg
     7681 gttgttcgat tgcctgctca atgagtacac gcccctgctc ttccacgccg gcttcacgca
     7741 attgctggtc gattgccgca cgttcattgg catcaatatg accatcactt ttagcggcaa
     7801 agaccagcgc aaggatcaaa cgttccgtac gctcatccag cggcgtactt tgcgcgccaa
     7861 actgcggttc gtcctgatgc gccgcgcgaa ttttatcttt gtatttattc cacagcaccg
     7921 tacccgctac cgctccgccg ccaaccagta acgcgttggt gccatatttc gtaagaagtt
     7981 tacgtgctga tttatttgcg accagcagcc cagccagtcc gcctaatgcg cctggcacta
     8041 acagtttgcc caatccctga tccgcagacg aggaggtaga agaactgctt tgcccaagca
     8101 gggattgcag ttgatttaac cagttagcca tatttgctcc tcaataacca tttttagtgc
     8161 ctgtagcata gcggatgagg atgtcagaaa atgtatatcg agacaaaaga tgcgcaaata
     8221 agcggggcgc taggccgtta ttcaaagaaa ttcgcgccag gtaaggtatt tactgtatat
     8281 caggtgataa gggtatttta ggtgaaagta gtttgtcgtt ttgagtggca ttgtttgatg
     8341 ctgattgccc gatgcgacgc tgttgcgtct tctcaggcct acgattgtgg tgcgttctgg
     8401 gacggacaag gcactcgcgc cgcatccgcc aacggtcaat cgaattatca gaagccatta
     8461 acgccgctga tattccgcca ttcccgcttt gcagtccacg ctgacctggt aatgaatatc
     8521 ggcactttta ccacgcacgg ttagcggtac tgaccattta tcatctttac cctgaatgtc
     8581 ctgcaaactg acccaggcca cgggatcggc ctgaccgaca attttttgat catctgccca
     8641 gcgcgccacg cgattttgtt gataatcacg ttttacgctc gcggcaattc cggtagcatc
     8701 cagaccttca cactttggga aagtgaccga cttgctggtt tcattattgg cagcgaaaac
     8761 tgatgcgcag gcagaaacca acaaccgccc taaaaacgcc cctctttttt tcatgttttt
     8821 ctccatagca caatgattca ggagaaagca tggtacaaat tgtcaggagc gcaagttgct
     8881 tcaggccgcg tgtgaggcat cttcctcttc ggattcagca ccgacgacca gtggggacgg
     8941 taatttgccg gttttcagaa tggtgctcag catatctttt tgttctgcca gccatagcga
     9001 aagggcttca cgttgctcgt cttccatttg cactggagaa tttgtcagct aggtgtcaag
     9061 cagatccgcc gtatcaagca ttttgtcata agcatcggct tcctttttgc tggtaaacga
     9121 cattttctct tcgccctcac gaatgactac gtatttaact tcaaccgcca tttgcagcct
     9181 ctcataataa ctgtgatttt atacagtata tttcttttcg gttgagaaat caacatcagc
     9241 aataaagaca cacgcaaacg ttttcgttta tactgcgcgc ggaattaatc aggggatatt
     9301 cgttatgacg ttattaggca ctgcgctgcg tccggcagca actcgcgtga tgttattagg
     9361 ctccggtgaa ctgggtaaag aagtggcaat cgagtgtcag cgtctcggcg tagaggtgat
     9421 tgccgtcgat cgctatgccg acgcaccagc catgcatgtc gcgcatcgct cccatgtcat
     9481 taatatgctt gatggtgatg cattacgccg tgtggttgaa ctggaaaaac cacattatat
     9541 cgtgccggag atcgaagcta ttgccaccga tatgctgatc caacttgaag aggaaggact
     9601 gaatgttgtc ccctgcgctc gcgcaacgaa attaacgatg aatcgcgagg gtatccgtcg
     9661 cctggcggca gaagagctgc agctgcccac ttccacttat cgttttgccg atagcaaaag
     9721 ccttttccgc gaggcggttg ccgccattgg ctatccctgc attgtaaaac cggtgatgag
     9781 ctcttccggc aaggggcaga cgtttattcg ctctgcagag caacttgctc aggcatggga
     9841 atacgctcag caaggcggtc gcgccggagc gggccgcgta attgttgaag gcgtcgttaa
     9901 gtttgacttc gaaattaccc tgctaaccgt cagcgcggtg gatggcgtcc atttctgtgc
     9961 accagtaggt catcgccagg aagatggcga ctaccgtgaa tcctggcaac cacagcaaat
    10021 gagcccgctt gcccttgaac gtgcgcagga gattgcccgt aaagtggtgc tggcactggg
    10081 cggttatggt ctgtttggtg tcgagctgtt tgtctgtggt gatgaggtga ttttcagtga
    10141 ggtctcccct cgtccacatg ataccgggat ggtgacgtta atttctcaag atctctcaga
    10201 gtttgccctg catgtacgtg ccttcctcgg acttcctgtt ggcgggatcc gtcagtatgg
    10261 tcctgcagct tctgccgtta ttctgccaca actgaccagt cagaatgtca cgtttgataa
    10321 tgtgcagaat gccgtaggcg cagatttgca gattcgttta tttggtaagc cggaaattga
    10381 tggcagccgt cgtctggggg tggtactggc tacttcagag agtgttgttg acgccattga
    10441 acgcgcgaag cacgccgccg gacaggtaaa agtacagggt taaacccggc aaaaaacgct
    10501 acaaaaatgc ccgatcctag atcgggcatt ttgactttta cagcttagcg ccttctacag
    10561 cttcacgcgc cagcttagta atgcggtcgt aatcgcccgc ttccagcgca tctgccggaa
    10621 ccagccagga accaccgatg cacagcacgc ttttcagtgc caggtagtca cggtagttag
    10681 ccggagaaat accacccgtc gggcagaaac ggacctggga gaacggaccc gcgatcgcct
    10741 gcagggcttt cacgccgccg ttagcttcag ccgggaagaa tttgaactct ttcaaaccgt
    10801 agtccatacc cagcatcagt tcggaaacag tgctgatccc cggaatcaga ggaatagtcc
    10861 cttcggtagc agctttcagc agcggctcgg tcagacccgg gctaattgcg aactgtgcac
    10921 ccgcttcagt gacttctgcc agctgctgtg gattcagcac cgtaccggca cccacaatcg
    10981 cttcaggcac ttctttggcg atagcacgga tagcgtcaac tgcacactcg gtacgcagag
    11041 tcacttccag aacgcgcacc ccaccagcaa ccaacgcttt tgccatcggc accgcgtgtt
    11101 ccagtttttt taccacgata accggtacaa ccgggccggt ggtcaggatt gattctgcac
    11161 ttgttttcca gtttttcatc agagttttct ctcgcctgat tacaaatttg tcgtcttaaa
    11221 aagtgataca ggttgcgccc tgttcggcac cggacagttt ttcacgcaag gcgctgaata
    11281 attcacgtcc tgttctcacg cgtgacgcgc tcaggtcagg aatgtgcggt tcgcgagcag
    11341 ccagttccgc ttcgtctacc agcagtgtca gttcgcctgt ctgtccattc acacgaatga
    11401 tgtccccgtc gcgcactttt gccagcagcc cgccatcgta ggcttctggt gttacgtgga
    11461 tagctgacgg cactttacct gaagcgccgg agagtcgtcc atcggtaact aacgcaattt
    11521 tgaaacaccg gtccaataat acaccaagtg gcggcatgag tttatgtaat tctggcattc
    11581 cgttcgcttt tggcccctga tgacggacaa caacgacaca atcgcggtcc agcaaacccg
    11641 cttcaaaggc cggcataacg tcatgctggc tttcaaaaac aaccgctggc gcttcaatca
    11701 cctggttctc aaccggcacg gcagaggttt tcataaccgc acggcccagg ttaccgctta
    11761 acacttttgt cccaccatga tgagagaaag gttgttcgaa ggaagcgatc acattgctgt
    11821 cgagtgattt ttccgcccct tcccgccagt ccagttcacc attattcagc catggttcaa
    11881 gggtataacg aaacagacca aagcctgcca ccgtattgac atcttcatgc agcaggcctg
    11941 ctttgagcag ttcacgcacc agaaccggta cgccacctgc cgcctggaag tggttaatat
    12001 cggccggacc gttcgggtag agacgtgcca tcagcggtac aacatcagaa aggtcagaga
    12061 agtcatccca gttaatctga ataccggccg cgcgcgccat cgccaccagg tgcatggtgt
    12121 ggttagtgga accaccggtc gccaacagtg caacgatacc gttcaccacc actttctcat
    12181 cgatcatctt accgatcggc atccattcat taccattacc ggtcatgcgt gtaacctgac
    12241 gcgcagcggc agcggtcaaa gcatcgcgca gcggagaatc cggatgaaca aaagaagagc
    12301 ctggcaactg catccccata aattccacca ccatctggtt ggtgttggca gtaccgtaga
    12361 aagtacatgt tcccggcgca tggtaagacg cggcttctga ctccagtaag gccatgcggt
    12421 ccactttacc ttcggcataa agctggcgaa tacgcacttt ttctttattt ggcaaaccgc
    12481 ttgccatcgg tccagacggc acaaacaccg caggcaaatg accaaacgac agggctgcca
    12541 tcgtcagacc cgggacaatc ttgtcgcaca caccgaggaa cagagcacca tcaaacatgt
    12601 tatgggacag ccccaccgcc gcagacatcg ctatcacttc gcggcttagc agcgacaatt
    12661 ccattccatc ctgcccctgg gtgacaccat cacacatcgc cggaacaccg cccgcaacct
    12721 gaccaaccgc attcgcttca tgcagggctt tacgaatgat ttctggatag tgttcataag
    12781 gctggtgcgc ggagagcatg tcgttatagg aggtgatgat ggcgatattg ttacgcaaca
    12841 tgcttttcaa agaggctttg tcttctggct ggcaggcagc gaaaccgtgt gccaggttac
    12901 cgcatgccaa ctgcgaacga tgaacggtcg acgttttcgc ttgttctatc cgggcgagat
    12961 aagcagagcg agtctcgcgc gaacgttcaa tgattcgatt tgttacgcgt aacaattgtg
    13021 gattcataaa ggctcctgaa attgagttgt cagagcagga tgattcacaa cgcgtttcat
    13081 tcagaggatt tatgagtgaa acgcctgtaa ccggagctca tagggcaaaa acgtttcagt
    13141 cagtgtaata aaaaaagcct cgtgggtgaa tccgcacgag gcctgaaagt gtaaaaattg
    13201 ttctacaatc tgtgcaagat tatgttaccg gtaaaataac cataaaggat aagcgctgat
    13261 attactcaaa ctcattccag gaacgaccat cacgggtaat catcgccacc gaggcaacgg
    13321 gtccccaggt tccggcctga tacggtttcg gcgcatcatt gtccatcgcc cacgcctcag
    13381 taatggagtc tacccatttc caggcttctt ccacttcgtc gcgacgtaca aacagtgcct
    13441 gaataccacg catggtttcc agcagcaaac gttcataggc atccgccaga tgcgtctgat
    13501 taaaggtttc tgaatagctc agatccagct tggtgatttg caggttatgt ttgtggtcaa
    13561 ggccaggaac tttattcagt acctggatat ccacgccttc atcaggttgc agacggatag
    13621 tcagtttatt ctgcggcaga tcctgccacg actctttaaa cagattcagt tcaggtgttt
    13681 tgaaatagac cacgacttca gaacatttgg tcggcagacg tttaccagta cgcaggtaga
    13741 atggcacacc ggcccagcgc cagttatcaa tgtcgacgcg gatcgccacg aaggtttctg
    13801 tattgctact tttgttcgcg ccctcttctt ccagatatcc cggcactttt ttgccctggg
    13861 cgaagcccgc agtatattgc ccgcgtacgg ttttttcgcg tacgttggag cggtcgatgc
    13921 ggcgcaggga cttcagtact ttcacttttt catcgcggat gctgtctgcg ctcaggtcag
    13981 atggcggaga catcgcaatc atgcagagaa tttgtagcag gtggttctga atcatgtcac
    14041 gcatctgacc ggctttatca aaatagcccc agcgcccttc gatccccact tcttctgcca
    14101 cggtaatctc aacatgatca atggtgcgat tgtcccagtt attcacaaac agggagttag
    14161 caaaacgcag cgccaacagg ttcagcaccg tttctttacc aagatagtgg tcgatacggt
    14221 aaacctggca ctcctcgaag tattcgccaa cctgatcatt gatttcctgc gaggtcgcca
    14281 gcgacgtccc cagcggtttc tccatgacta cgcgtgccgg tttagcattc agttttgcct
    14341 cgccaagccc tttgcaaatt gcgccaaaag tgctgggcgg catggcaaag tagttaatag
    14401 tgatacgatt tttttgatcc agcatcgcgc cgagacggtt gaatgcagca gtgtcattga
    14461 catcgagatt acaaaaatcc agacgtgcac tcagggtgtc ccataaacct tcatcaatgg
    14521 tttctttcat gaaagtttcg agcgcctcgc ggacaacttt ggtatatgcc gctttatccc
    14581 agtcagcacg ccctacgccg ataatccggg tgtccgggtt gagctgaccg gctttttcca
    14641 gttgatacag ggaaggcagc aatttacgac gcgcaaggtc gcctttcgcg ccgaaaatga
    14701 ccaggtcaca ggcctgggct gtttgcgtta ccgccatgtc attctcctta agttaactaa
    14761 cccggtactt aagccagggt atacttgtaa ttttcttacg gtgcactgta ctgcttttcc
    14821 gagcttgcga aaactgtaaa cgcttatcca cccgtgcgat tacgggaaaa gcgcgcaaag
    14881 tgcggcaaaa ctgataaaaa aatcatcgtt ttccattcgg taaaaatctg acactgatca
    14941 tgttatgaaa aaaaataaca acttttttat ctgcttttgt cattaacacg gcacacaggc
    15001 gtaatatctg acaaaactgc atttcgattt ctttcagtgc ggaaatcgtc attacccgtg
    15061 agtctcttta catcatgaat atgctggaaa aaatccagtc tcagctggaa catttgagca
    15121 aatcagagcg caaagttgcc gaggtcattc tggcttcgcc cgataacgcg atccattcga
    15181 gtattgctgc tctggcgctg gaagccaatg ttagcgaacc gacggtgaat cgtttctgtc
    15241 gcagcatgga cacgcgcggt tttcctgatt ttaaacttca tctggcacaa agtctggcga
    15301 atggcactcc ctatgttaat cgcaacgtca atgaagatga cagcgttgaa tcatacacgg
    15361 ggaaaatatt tgagtccgca atggcaacgc tcgatcatgt ccgtcattca ctggataaat
    15421 ctgccatcaa ccgcgccgtc gacttgctca ctcaggcaaa aaaaatcgcc tttttcggat
    15481 taggctcttc tgccgccgtt gcccacgatg cgatgaataa gttctttcgt tttaatgttc
    15541 cggtggtgta ctccgatgat atcgtgctgc aacgcatgag ttgtatgaat tgtagcgacg
    15601 gagacgtggt ggtgctgatt tctcacacag gaagaacaaa aaatctggtc gagctggcgc
    15661 agctggcacg cgaaaacgac gccatggtga ttgccctcac ctctgcgggt accccgctcg
    15721 cccgggaagc aacgctggca attaccctcg acgtaccgga agatactgac atttatatgc
    15781 ccatggtttc tcgacttgca cagctgaccg tgatagatgt gctggcgaca ggatttactt
    15841 tgcgacgcgg tgcaaaattc agagataact tgaagcgggt caaggaagcg ctgaaggaat
    15901 cgcgttttga taagcagtta cttaatttaa gtgacgatcg ctaaaagcga ctgtcactgt
    15961 cctaatctta tacgacatcc gaatgagatt aatttatcgc catcgcggcg ttatttcatt
    16021 cggatttcat gttcaagcaa cacctggttg tttcagtcaa cggagtatta catgtccaaa
    16081 aggcttcgca gaacaaaaat cgttaccacg ttaggcccag caacagatcg cgataataat
    16141 cttgaaaaag ttatcgcggc gggtgccaac gttgtacgta tgaacttttc tcacggctcg
    16201 cctgaagatc acaaaatgca cgcggataaa gttcgtgaga ttgccgcaaa actggggcgt
    16261 catgtggcta ttctgggtga cctccagggg cccaaaatcc gtgtatccac ctttaaagaa
    16321 ggcaaagttt tcctcaatat tggggataaa ttcctgctcg acgccaacct aggtaaaggt
    16381 gaaggcgaca aagaaaaagt cggtatcgac tacaaaggcc tgcctgctga cgtcgtgcct
    16441 ggtgacatcc tgctgctgga cgatggtcgc gtccagttaa aagtactgga agttcagggc
    16501 atgaaagtgt tcaccgaagt caccgtcggt ggtcccctct ccaacaataa aggtatcaac
    16561 aaacttggcg gcggtttgtc ggctgaagcg ctgaccgaaa aagacaaagc agacattaag
    16621 actgcggcgt tgattggcgt agattacctg gctgtctcct tcccacgctg tggcgaagat
    16681 ctgaactatg cccgtcgcct ggcacgcgat gcaggatgtg atgcgaaaat tgttgccaag
    16741 gttgaacgtg cggaagccgt ttgcagccag gatgcaatgg atgacatcat cctcgcctct
    16801 gacgtggtaa tggttgcacg tggcgacctc ggtgtggaaa ttggcgaccc ggaactggtc
    16861 ggcattcaga aagcgttgat ccgtcgtgcg cgtcagctaa accgagcggt aatcacggcg
    16921 acccagatga tggagtcaat gattactaac ccgatgccga cgcgtgcaga agtcatggac
    16981 gtagcaaacg ccgttctgga tggtactgac gctgtgatgc tgtctgcaga aactgccgct
    17041 gggcagtatc cgtcagaaac tgttgcagcc atggcgcgcg tttgcctggg tgcggaaaaa
    17101 atcccgagca tcaacgtttc taaacaccgt ctggacgttc agttcgacaa tgtggaagaa
    17161 gctattgcca tgtcggcaat gtacgcagct aaccacctga agggcgttac ggcgatcatt
    17221 accatgaccg aatcgggtcg taccgcgctg atgacctccc gtatcagctc tggtctacca
    17281 attttcgcca tgtcgcgcca tgaacgtacg ctgaacctga ctgctctcta tcgtggcgtt
    17341 acgccggtgc actttgatag cgctaatgac ggcgtagcag ctgccagcga agcggttaat
    17401 ctgctgcgcg ataaaggtta cttgatgtct ggtgacctag tgattgtcac ccagggcgac
    17461 gtgatgagta ccgtgggttc tactaatacc acgcgtattt taacggtaga gtaagtacgt
    17521 tgccggatgc ggcgaaaacg ccacatccgg cctatagttc aattatagtt caacagattt
    17581 cgaatattct gaagcaaact tgaacttatc atcaggcgaa ggcctctcct cgcgagaggc
    17641 ttttttattt gatgggataa agatctttgc gcttatacgg ctggatttcg cccggtttgc
    17701 gagttttcag caattttagt atccaggtgt attgttctgg tcgcggacca acaaaaatct
    17761 cgacttcttc attcatccgc cgctcaatcg tatgatcatc cgcctctaac agatcatcca
    17821 tcggtgggcg cacctgaatc gtcagacgat gcgtcttgcc atcataaatc ggaaacagcg
    17881 gtacaacgcg cgcacggcat actttcatca aacgaccaat cgcgggcaac gtcgctttat
    17941 aggtggcaaa gaaatccaca aattcgctgt gctctgggcc atgatcctga tcgggtaaat
    18001 aatatcccca gtacccctga cgtaccgact ggatgaatgg tttaataccg tcatttctcg
    18061 catgcagacg accgccaaag cgacgacgca ccgtgttcca gacataatca aaaaccgggt
    18121 tgccctgatt atggaacatc gctgccattt tctgcccttg cgaggccatc agcatggcag
    18181 gaatatcgac ggcccaaccg tgcggcacca gaaagataac tttctcgtta ttacgccgca
    18241 tctcttcgat gatctccagc ccttgccagc caacgcgcgg ctgaattttc tccggcccgc
    18301 gtattgccaa ctcagccatc attgccatcg cttgcggcgc ggtggcaaac atctcatcaa
    18361 caatcgcttc gcgttcagct tcactacgtt ctggaaagca gagcgacaga ttgattaacg
    18421 cacgacggcg tgagcttttt cccagtcgtc cggcaaaacg tcccagccgt gccagaatgg
    18481 gatcacggaa ctttggcggc gttaaagcga tacccgccat cgctgctaca cccagccatg
    18541 ctccccagta gcgcgggtgg cgaaaggatt tatcaaactc aggaatgtat tcgctattat
    18601 tttttttcgt ttccatgctt ttccagtttc ggataaggca aaaatcaatc tggtgatagt
    18661 gtagcggcgc aacttgcccc gcaccaaata aaaaaagccg gtactgactg cgtaccggct
    18721 gcgaatggat gttaattaat caaacagtag ctgcggcaca atctctttgg cctgcgccag
    18781 gaattcgcga cgatcggagc cggtcagccc ttcggtacgc ggcagttttg ccgtcagcgg
    18841 gtttacggcc tgctggttta tccatacttc atagtgcaga tgcggcccag ttgaacgtcc
    18901 ggtattaccg gaaagcgcga tacggtcgcc acgtttcacc ttctgtcccg gtttcaccag
    18961 aatcttgcgc aagtgcatat aacgcgtggt gtagctgcga ccatgacgaa tagccacata
    19021 ataacctgct gcgccactgc gtttggcaac caccacttca ccgtcaccca ctgaaagcac
    19081 tggcgtacct tgtggcatgg cgaaatcaac acctctgtgt ggtgcaacgc gacctgtcac
    19141 cggattagtt cgacgcgggt taaagttaga cgagatacgg aactgtttcg ccgtcgggaa
    19201 tcgcaagaat cctttcgcca gaccagtacc gttacgatcg tagaatttgc catcctcagc
    19261 gcggattgcg taataatctt taccttctga acgcaaacgt acgcccagca gctggctttg
    19321 ctcacgttta ccatcaagca tttctcgaga cattaatacc gcaaattcat cgcctttttt
    19381 cagtttgcgg aaatccattt gccactgcat ggctttaatc actgcgctca cttcggcgct
    19441 ggttaaaccg gcgtttctgg cgctggcaac aaagctccct ccgacggtac ctttcagcag
    19501 attgttaacc cactctcctt gctgcatttc gctggtcatt ttaaaaccgt tagcggcagt
    19561 acggtcatag gttcgggttt cacgacgaga cacttcccag gtgaggcgct gcagttcgcc
    19621 gtccgcggtt aatgtccagg agagttgttg accgattttc aggttacgca attctttgtc
    19681 ggcagcagcg agttgggtga tatcacccat atcaatacca tactgattga gaatgctgct
    19741 tagcgtatcg ccagtggaaa caacatattc atgcacgccc gcttcaccgg cgattttgtc
    19801 atccagttcg tcctggggaa tggcttcatc ttcttgtgca gcttgatcaa tcggctcact
    19861 ggcttcaggt aagagcgaac gaatttcgtt ctgttccagc tcaatggttt tgacaattgg
    19921 cgtggcatca cggtgataaa catagggccg ccagacagcg acggccagag taagaacggt
    19981 gagcgacccc aacataacgc ggtgtggtcg cggtaaatta ttaaacgcca gggcgacaga
    20041 gcgggctatc tgttgcacgt aatcacttcc tcattaatct cctttcaggc agctcgcata
    20101 ctggttggct aattgactca ggaattctga atagcttgtt ttacccagtt tgatattcgt
    20161 ccccagggga tccaacgttc ccatacgaac ggatgtccct cgtgcgacgc tctcaacgac
    20221 cgctggcctg aactgtggct cagcaaaaac gcaggttgct ttttgctcaa ccaactgtgt
    20281 tcttatttca tgtaaacgct gcgcgccagg ttgaatctca gggttaacgg taaaatgacc
    20341 aagcggtgtc agtccgaact gtttttcgaa atagccgtaa gcatcgtgaa aaacgaaata
    20401 acctttcccc ttgagcggcg cgagctcgtt accaacctgc gtttcggttg aggccaattg
    20461 tgcctcaaaa tccttcaggt tggcgtcaag tttggctcga ctttgcggca taagttccac
    20521 taattttcca tggattgcaa ccgctgtagc ccgcgctatc tctggggaaa gccaaagatg
    20581 catgttgaaa tcgccgtgat ggtgatcttc gtcacttttt tccgcgtggt cgtgatcatc
    20641 atcatcgccg tgaatacttt tcatcagtag cggtttcaca tcttcaagct gcgcaatcgt
    20701 tacctgcttc gcttctggta atttgcttac cggtttttgc ataaacgctt ccatctccgg
    20761 gccaacccaa acgactaagt ccgcgttctg taagcgtttt acatccgatg gacgcagtga
    20821 ataatcatgt tctgaagcgc cgtcaggcag taaaacctcc gtttctgtta caccatcagc
    20881 aatggcagaa gcgatgaacc caacgggttt aagcgaagcg acaacggcgg catctgcggc
    20941 ctgtgttgca cctccccaga gagcggcgga taatgctgcg aaaagaagcg tttttttatg
    21001 taacataatg cgaccaatca tcgtaatgaa tatgagaagt gtgatattat aacatttcat
    21061 gactactgca agactaaaat taacatgaca agtctggttt ccctggaaaa tgtctcggtt
    21121 tcttttggcc aacgccgcgt cctctctgat gtgtcgctgg aacttaaacc tggaaaaatt
    21181 ttgactttac ttgggccaaa tggcgcaggt aagtcgacac tggtacgggt agtgctcggg
    21241 ctggtaacac ccgatgaagg ggttatcaag cgcaacggaa aactgcgcat cggctatgta
    21301 ccgcagaagc tgtatctcga caccacgttg ccactgaccg taaaccgttt tttacgctta
    21361 cgccctggta cacataaaga agatattttg cctgcactga aacgtgtcca ggccgggcac
    21421 ctgattaacg caccgatgca aaagctctct ggtggcgaaa cgcagcgtgt actattagcg
    21481 cgagcattgt taaattgccc gcaattatta gtgctggatg aacccactca aggcgtggat
    21541 gtaaatggcc aagtggcgtt atatgacctt attgaccagt tgcgtcgtga actggattgt
    21601 ggcgttttaa tggtttctca cgatctgcat ctggtgatgg caaaaaccga tgaagtgctg
    21661 tgcctgaatc accacatttg ttgttccggc acaccggaag ttgtttccct gcatccggag
    21721 tttatttcaa tgtttggtcc tcgtggtgct gaacaactgg gtatctatcg ccatcatcat
    21781 aatcatcgtc acgatttaca gggacgaatt gttttgcgtc ggggaaatga tcgctcatga
    21841 ttgaattatt atttcccggt tggttagccg ggatcatgct cgcctgtgcc gcgggtccgc
    21901 tgggttcgtt tgtagtctgg cgtcgtatgt cttatttcgg tgatacgctg gctcatgcct
    21961 cattacttgg tgtcgcgttt ggtttgttgc tggacgtgaa tccattctat gcggtgattg
    22021 ccgttacgct gctgctggcg ggcggtctgg tatggctgga gaagcgtcca cagctggcga
    22081 tcgacacgtt attagggatt atggcgcaca gtgccctgtc gctgggccta gtggtcgtta
    22141 gtctgatgtc taacattcgt gttgatttga tggcttacct gttcggtgat ttactggcag
    22201 tgacgccaga agatctcatc tctattgcga ttggcgtggt catcgtggtg gctattttgt
    22261 tctggcaatg gcgcaatttg ctgtcgatga cgattagccc ggatctggcg tttgttgatg
    22321 gtgtgaaatt acagcaagtg aaattgttgt tgatgctggt gacggcattg acgattggtg
    22381 tagcgatgaa atttgtcggt gcgttgatta ttacttcgtt gctgattatt cctgcagcta
    22441 ctgcgcgtcg ctttgcccgt acgccggaac agatggctgg tgtcgctgtt ttggtgggga
    22501 tggtggcagt aactggcggt ttaacctttt ccgcgtttta cgatacgccg gcgggtccgt
    22561 cggtggtgct atgtgcggca ctgttattta ttatcagtat gatgaaaaag caggccagct
    22621 aatctatcgc tgaacacatt tgtcggatgc ggcgcgagcg ccttatccga cctacggttc
    22681 gctatctctg gctggcctga taagacgcga acagtgtcgc atcagacaca ctgccagtgt
    22741 cggatgcggc gcgagcgacc aatccgactt acggcatttc tggcggcgtt atgccaaagt
    22801 gattccacgc ccgcgtcgtc gccatacgcc cgcgcggtgt acgctgcaaa aagccttgct
    22861 gaatcaaata aggttccagt acatcttcaa tggtttcacg ttcttcgcca atggctgccg
    22921 ccaggttatc cagacctacc ggcccaccaa agaacttatc gattaccgcc agcaacaatt
    22981 tgcggtccat ataatcgaaa ccttcagcat cgacattcaa catatccagc gcctgagcag
    23041 cgatatctgc cgagatggtg ccatcgtgct tcacttcggc gaaatcacgc actcgacgca
    23101 gcagacggtt ggcaatacgt ggcgtaccgc gagcgcgacg agctacttcc tgcgcaccgt
    23161 catcactcat ctcaagcccc ataaagcgtg cactgcgact gacgatatat tgcagatccg
    23221 gcacctgata aaactccaga cgttgcacaa taccaaaacg gtcgcgcaac ggtgatgtca
    23281 gcgaacctgc gcgcgtggtt gcgccaatca gggtaaacgg cggcaaatca attttaatgg
    23341 agcgtgccgc cggaccttca ccaatcatga tatccagttg gtagtcttcc attgccgggt
    23401 acagcacttc ttcaacaact ggcgataaac ggtggatctc atcaataaac agcacgtcat
    23461 gcggttcaag gttagtgagc atcgcagcca aatcgcccgc cttttccagc accggaccag
    23521 aagtcgtgcg taaattaacg cccatttcat tggcgacaat gttggcaagc gtagttttac
    23581 ccaaccccgg aggaccaaaa atcaacaaat gatcgagggc atcgccgcgc agtttcgctg
    23641 ctttgatgaa aatctccatc tgtgaacgaa cctgcggctg accaacatac tcttccagta
    23701 atttagggcg aatggcgcga tctgctacat cttccggcaa agtggtaccg gcagaaatca
    23761 gacggtctgc ttcaatcatc ctttacctca taacgcggcg cgtagggctt cgcgaattaa
    23821 tgtttcactg ctggcgtcag ggcgagcgat tttgctcacc atgcggcttg cttcttgtgg
    23881 tttatagcct agcgccacca gcgcggcaac tgcttcttgt tcagcatcgt cggtcgccgg
    23941 gctggcagga gacgtgagta ccaggtcggc ggctggcgta aagagatcgc catgcaaacc
    24001 tttaaatcgg tctttcattt caacaatcaa gcgttcggcg gtttttttgc caatacccgg
    24061 cagtttcacc agtgccccca cttcttcacg ctcaacggca ttaacgaact gctgcgctga
    24121 cattccggag aggatcgcca gggccaactt cgggccgacg ccgttggttt tgatcaactc
    24181 tttgaacaac gtgcgttctt gtttattgtt aaaaccgtac agcagttgcg cgtcttcacg
    24241 caccacaaag tgggtgaaaa cgatcgcttc ctgacccgct tcagggagtt cataaaaaca
    24301 ggtcatcggc atatgcactt catagcctac gccgcccact tcaattaaca ccagcggggg
    24361 ttgtttttca atgatgatgc ctctgagtct gcctatcaca tgacgctcct gcgtaatgaa
    24421 tcaaagataa tgctgtatga taaaaaaatg ctggatagat atccagcgta ggatgaagaa
    24481 aacttgcgag gtgtcccgat gatctgaaaa atggcacagt ataatttatt caacaggtta
    24541 tattggaagc aaatatttga atattacata ttcagtgaag aaatgcgtaa taaaaatata
    24601 cattgcgact cctgaaaaaa taattttttt atgctattac gtatattcgt atctatttca
    24661 atggaatgac aacgtgaata ttaattaccc tgctgaatat gaaattggag atatcgtctt
    24721 tacatgtata ggtgctgcct tatttggtca aatatcagct gcatcaaatt gctggagtaa
    24781 tcacgtcggg atcattatcg gtcataacgg tgaagacttc ctggttgcag aaagccgggt
    24841 tcccctttca accatcacca
//
LOCUS       DADMGC010000053        24798 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.053, whole genome shotgun sequence.
ACCESSION   DADMGC010000053 DADMGC010000000
VERSION     DADMGC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.053"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(51..884)
                     /gene="fdhD"
                     /locus_tag="JNZ73_002090"
     CDS             complement(51..884)
                     /gene="fdhD"
                     /locus_tag="JNZ73_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAY8732376.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            1078..4128
                     /gene="fdnG"
                     /locus_tag="JNZ73_002091"
     CDS             1078..4128
                     /gene="fdnG"
                     /locus_tag="JNZ73_002091"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1663..1665,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY8732377.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMFSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGITLTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            4141..5043
                     /gene="fdxH"
                     /locus_tag="JNZ73_002092"
     CDS             4141..5043
                     /gene="fdxH"
                     /locus_tag="JNZ73_002092"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY8732378.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            5040..5675
                     /gene="fdoI"
                     /locus_tag="JNZ73_002093"
     CDS             5040..5675
                     /gene="fdoI"
                     /locus_tag="JNZ73_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY8732379.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            5672..6601
                     /gene="fdhE"
                     /locus_tag="JNZ73_002094"
     CDS             5672..6601
                     /gene="fdhE"
                     /locus_tag="JNZ73_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HAY8732380.1"
                     /translation="MSIRIIPQDELGSSEKRTANMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(6931..7173)
                     /locus_tag="JNZ73_002095"
     CDS             complement(6931..7173)
                     /locus_tag="JNZ73_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732381.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(7391..7609)
                     /locus_tag="JNZ73_002096"
     CDS             complement(7391..7609)
                     /locus_tag="JNZ73_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HAY8732382.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(8288..9433)
                     /locus_tag="JNZ73_002097"
     CDS             complement(8288..9433)
                     /locus_tag="JNZ73_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732383.1"
                     /translation="MCERMKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSA
                     KDVGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPK
                     NNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAH
                     SHRSVALAKTVAAPIMATVVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQS
                     DISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGC
                     KSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKNLF
                     DIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            complement(9591..9929)
                     /locus_tag="JNZ73_002098"
     CDS             complement(9591..9929)
                     /locus_tag="JNZ73_002098"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732384.1"
                     /translation="MSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            complement(10519..11460)
                     /gene="fabY"
                     /locus_tag="JNZ73_002099"
                     /gene_synonym="yiiD"
     CDS             complement(10519..11460)
                     /gene="fabY"
                     /locus_tag="JNZ73_002099"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HAY8732385.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(11505..11942)
                     /gene="dtd"
                     /locus_tag="JNZ73_002100"
     CDS             complement(11505..11942)
                     /gene="dtd"
                     /locus_tag="JNZ73_002100"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAY8732386.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(11939..12811)
                     /locus_tag="JNZ73_002101"
     CDS             complement(11939..12811)
                     /locus_tag="JNZ73_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HAY8732387.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(12805..13404)
                     /gene="yihX"
                     /locus_tag="JNZ73_002102"
     CDS             complement(12805..13404)
                     /gene="yihX"
                     /locus_tag="JNZ73_002102"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HAY8732388.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(13503..14287)
                     /locus_tag="JNZ73_002103"
                     /pseudo
     CDS             complement(13503..14287)
                     /locus_tag="JNZ73_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            complement(14321..15217)
                     /gene="yihV"
                     /locus_tag="JNZ73_002104"
     CDS             complement(14321..15217)
                     /gene="yihV"
                     /locus_tag="JNZ73_002104"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HAY8732389.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGVKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            15385..17040
                     /gene="yihU"
                     /locus_tag="JNZ73_002105"
     CDS             15385..17040
                     /gene="yihU"
                     /locus_tag="JNZ73_002105"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HAY8732390.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPVQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARASGGFA
                     MLAVDQREAMRMMFAAAGAPAPVADSVLTDFKVNAAKALSPYASAILVDQQFCYRQVV
                     EQNAIAKSCAMIVAADEFIPGNGIPVDSVVIDRKINPLQIKQDGGKALKLLVLWRSDE
                     DAQQRLDMVKEFNGLCHSHGLVSIIEPVVRPPRRGDKFDREQAVIDAAKELGDSGADL
                     YKVEMPLYGKGPQQELLCASQRLNDHINMPWVILSSSVDEKLFPRAVRVAMTAGASGF
                     LAGRAVWASVVGLPDNELMLRDVCAPKLQQLGDIVDEMMAKRR"
     gene            17057..18298
                     /gene="yihS"
                     /locus_tag="JNZ73_002106"
     CDS             17057..18298
                     /gene="yihS"
                     /locus_tag="JNZ73_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HAY8732391.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWDPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            18412..19338
                     /locus_tag="JNZ73_002107"
     CDS             18412..19338
                     /locus_tag="JNZ73_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HAY8732392.1"
                     /translation="MSLIKVPCMQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTF
                     QGCHLVIPHKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGL
                     LAWRDWQISELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTV
                     AAPYGVGIHPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKK
                     IAATKIDHTFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMS
                     CPPNAFNSGIDLLLLEPGKPHRLFFNIYGQRK"
     gene            19537..21573
                     /gene="yihQ"
                     /locus_tag="JNZ73_002108"
                     /pseudo
     CDS             19537..21573
                     /gene="yihQ"
                     /locus_tag="JNZ73_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
     gene            21619..23004
                     /locus_tag="JNZ73_002109"
     CDS             21619..23004
                     /locus_tag="JNZ73_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8732393.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQKLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            23047..24450
                     /locus_tag="JNZ73_002110"
     CDS             23047..24450
                     /locus_tag="JNZ73_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8732394.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            24518..>24798
                     /locus_tag="JNZ73_002111"
     CDS             24518..>24798
                     /locus_tag="JNZ73_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732395.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGG"
BASE COUNT         6138 a         6380 c         6091 g         6189 t
ORIGIN      
        1 cgaaatgaat aagtcctgca atttaatata tatcgcagga cttattttaa ttaattgctc
       61 aaacgctgcg gatgggtata aaccgttgcc cgacccggtt tacaaaaacc taccagtgtc
      121 agattacagc gctcagccac ttctacagca agcgtggtcg cggcagacac cgcaaacaaa
      181 atctctacgc cgcacatcgc cgacttttgc accatttcat aactggcacg actggaaacc
      241 agtaccgcac cttgctgcca gctttcccct tcttgtgacc ggcggcctaa cagtttgtcc
      301 agcgccacat ggcgacccac gtcttcatgc ccgccgacca gttcgccaga tggcaacatc
      361 caggcagcgg cgtgagtaca accagtcagt tgccccactg gctgaaaatc gttgagatga
      421 cgtaatgcat catccagttt gttgagatca aacgtctggg tgaacggtag cggctgcacc
      481 ggttttccga tgtcattaag ttgctccacg ccgcatacgc cacagcccgt acgtcccgcc
      541 agcgcccggc ggcgctcctt caaccccata aagcggcggc tggaaagctc aatttgtact
      601 tcaagaccat tacaggaagg aacgacatcc atgccgaaga tatcgcgcgg actttctatt
      661 atcccctcgg aaagcgaaaa accgagcgca aagtactcaa ggtctttggg cgacgccatc
      721 atcaccacat gcgaaatgcc gttgtagacc agcgcaacgg gaacttcttc cgcgacctca
      781 tctaaccgtg ggtgttgtaa atcatcacgc cgccataact caatttgacg aacacctgtg
      841 atatttgtca cattttcgat ttcttttcgc tgtgtttttt tcactttctc ttaaccagat
      901 aagaacacac acaccaacat tatggtattc tgttacaaac ccttcctaga tggagggaaa
      961 ttgagccaat tctggacctt tgcggcccct tccgcaaaga aaaataactc ccactccctg
     1021 cacacgcagc aagcgaatgt aaatgggacg tgacaatgtc gaaacaagga gcaatccatg
     1081 caggtcagca gaaggcagtt ctttaagatc tgcgctggcg gtatggcagg caccacggca
     1141 gcggcactgg gttttgcacc cagcgtagca ctcgcggaaa cccggcagta taaactgctg
     1201 cgcacccgcg aaacccgtaa tacctgcacc tattgttccg taggctgtgg gctgttgatg
     1261 ttcagcctcg gtgacggagc aaaaaacgcc aaagcatcta tcttccatat cgaaggcgat
     1321 ccggatcacc cggtcaaccg cggtgcactt tgtccgaaag gcgctggcct ggtggatttc
     1381 atccactccg aaagccgtct gaagtttccg gaataccgtg cgccaggttc tgataaatgg
     1441 cagcaaatca gttgggaaga ggcgtttgat cgcatcgcca aactgatgaa agaagaccgc
     1501 gatgctaact acattgcgca aaacgccgaa ggcgtgactg ttaaccgctg gctctccacc
     1561 gggatgctgt gtgcttccgc gtcgagtaac gaaaccggct atttaacgca aaaattctcc
     1621 cgcgcgctgg gtatgctcgc ggtcgacaac caggcgcgtg tctgacacgg accaacggta
     1681 gcaagtcttg ctccaacatt tggtcgcggt gcgatgacca accactgggt cgacatcaag
     1741 aacgccaacc tcgtcgtggt gatgggcggt aacgccgctg aagctcaccc ggtcgggttc
     1801 cgctgggcga tggaagccaa aattcacaac ggcgcgaagc tgattgtgat cgatcctcgc
     1861 tttacgcgta cggcggcggt agctgactac tatgccccta ttcgttccgg tactgacatt
     1921 gctttcctgt caggcgtatt gctgtacctg ctgaacaatg aaaaattcaa ccacgaatac
     1981 accgaagcct ataccaacgc cagcctgatc gtgcgtgagg attacggctt tgaagatggc
     2041 ctgttcaccg gctacgacgc ggaaaaacgc aagtacgata aatcctcctg gacttatgaa
     2101 ctggacgaaa acggcttcgc caaacgcgat accacgctgc aacatccgcg ctgcgtgtgg
     2161 aacttgctga aacagcacgt ttcccgctac acgccagatg tggttgaaaa catctgtggt
     2221 acgccaaaag acgcgttcct gaaagtctgc gaatacatcg cagaaaccag tgctcacgat
     2281 aaaactgcct cgttcctgta tgccctcggc tggacgcaac actccgttgg tgcgcaaaac
     2341 attcgtacga tggcgatgat ccagctgctg ctcggcaata tggggatggc cggcggcggc
     2401 gttaacgccc tgcgcggtca ctccaatatt caggggctga cggacctggg gctactgtcg
     2461 cagagtctgc caggttacat gacgctgcca agcgagaagc agaccgatct gcaaacctac
     2521 cttaccgcca acacgccaaa accgctgctg gaaggccagg taaactactg gggcaactac
     2581 ccgaaattct tcgtctctat gatgaaggcc ttctttggtg ataaagcgac ggcggaaaat
     2641 agctggggct ttgactggtt gccgaagtgg gataaaggct acgacgtcct gcaatacttc
     2701 gagatgatga aagagggcaa ggtcaatggc tatatctgcc agggctttaa ccctgttgcc
     2761 tcattcccga acaaaaacaa agtgatcggt tgtctgtcga aactgaagtt cctcgtcacc
     2821 atcgacccac tgaacactga aacctctaac ttctggcaga accacggtga gctgaacgaa
     2881 gttgactcgt cgaagatcca gaccgaagtg ttccgtctgc catcgacctg cttcgcagaa
     2941 gagaacggtt caatcgttaa ctcaggtcgc tggttgcagt ggcactggaa aggtgcggac
     3001 gccccgggga ttacgctgac tgacggtgag atcctctccg gtatcttcct gcgcttgcgc
     3061 aagatgtatg ccgaacaggg tggcgcgaac ccggatcagg tgctgaacat gacctggaac
     3121 tacgccattc cgcatgagcc gaagtcggaa gaagtagcga tggagagcaa cggtaaggcg
     3181 ctggccgata ttaccgatcc ggcaaccggt gcggttatcg tcaagaaagg ccaacaactt
     3241 agctcgttcg cccaattgcg tgatgacggt acaacctcct gtggctgctg gattttcgcc
     3301 ggtagctgga cgccggaagg caaccagatg gcgcgtcgtg ataacgccga tccgtctggc
     3361 ctcggtaaca cgctgggctg ggcatgggca tggccgctta accgccgcat tctgtataac
     3421 cgcgcctccg cagatccgca gggtaacccg tgggatccga agcgtcagtt gctgaaatgg
     3481 gacggcacta agtggaccgg ctgggatatt ccggactaca gcgcagcgcc tccgggtagc
     3541 ggcgtcgggc cgtttatcat gcagcaggaa ggcatggggc gtctgtttgc cctcgataag
     3601 atggcggaag gtccgttccc ggaacactac gagccgtttg aaacgccgct gggaactaac
     3661 ccgctgcatc caaacgttat ctcgaacccg gctgcacgta tctttaaaga cgacgccgaa
     3721 gcattgggta aagccgataa gttcccgtat gtcggaacca cctatcgtct gaccgagcac
     3781 ttccactact ggaccaaaca cgcgctgttg aacgcgattt tgcagccaga gcagtttgtg
     3841 gaaatcgggg agtcgctggc gaataaactt ggcattgccc agggcgatac cgtgaaagtc
     3901 tcctccaacc gtggctatat caaagccaaa gcggtggtga ccaaacgtat tcgcacgctg
     3961 aaagcgaacg gcaaagatat cgataccatc ggtattccga ttcactgggg ctatgaaggc
     4021 gtagctaaaa aaggctttat tgccaatact ttaacgccat tcgttggtga tgcgaacacg
     4081 cagacgccgg agtttaagtc cttccttgtg aatgtggaaa aggtgtaacg gagacgacat
     4141 atggcttatc aatctcaaga tatcattcgt cgttccgcga ctaacggtct gacccccgcg
     4201 cctcaggcgc gggacttcca ggaagaagtg gcgaaactca tcgacgttac cacctgtatc
     4261 ggctgtaaag cctgtcaggt ggcgtgttca gagtggaacg acattcgcga taccgtcggc
     4321 aataacattg gggtgtacga caaccccaat gatttaagcg ccaaatcgtg gacggtaatg
     4381 cgcttctcgg aagtggagca gaacgacaaa ctggaatggc tgatccgcaa agacggctgt
     4441 atgcactgtt ccgatccagg ctgcctgaaa gcgtgcccgg cggaaggtgc aattattcag
     4501 tatgccaacg gtatcgtcga cttccagtcc gagcagtgca ttggctgcgg ttattgcatt
     4561 gcgggctgtc cgttcgacat tccgcgcctc aacccggaag acaaccgcgt ctacaaatgt
     4621 acgctgtgcg ttgaccgcgt ggtggttggg caagaaccag cctgcgtgaa gacctgccca
     4681 acgggcgcga ttcacttcgg tacgaaagag tcgatgaaaa cgctggcgag cgagcgcgtt
     4741 gctgagctga aaacccgcgg ttacgacaat gcgggtctgt acgatccggc aggcgtcggt
     4801 ggtacacacg tcatgtacgt gctgcaccat gctgacaagc caaatctgta tcatggcttg
     4861 ccggagaacc cggaaatcag cgaaaccgtg aaattctgga aaggcatctg gaaaccgctc
     4921 gcggctgttg gctttgcggc tacctttgcg gccagtatct tccactacgt gggtgtcggt
     4981 ccgaaccgtg cggatgagga agagaataat ctgcacgaag agaaagacga ggagcgcaaa
     5041 tgaaacgacg tgacaccatc gtgcgctaca ccgcgccgga acgtatcaac cactggatca
     5101 ccgccttctg cttcatcctg gcggcggtga gcgggctggg ctttttgttc ccgtccttca
     5161 actggttgat gcaaatcatg ggcacaccgc agctggcgcg aattctgcac ccgtttgtcg
     5221 gcgtggttat gtttgcctcg ttcatcatca tgtttttccg ttactggcat caaaacctaa
     5281 tcaatcggga tgatatcttt tgggcgaaga atattcgtaa gatcgtcgtc aacgaggaag
     5341 taggtgacac cgggcgttat aacttcggtc agaaatgcgt tttctgggcg gcgattattt
     5401 tcctggttct gctgctggtg agcggcgtga ttatctggcg tccttatttt gcgcctgctt
     5461 tctcaatccc ggtgatccga ttcgcgttaa tgctgcattc atttgccgca gtggcgttaa
     5521 ttgtggttat catggtgcat atctacgccg ccctttgggt gaaaggcacg attaccgcca
     5581 tggtggaagg atgggttacc agcgcctggg cgaagaaaca tcacccgcgc tggtaccgtg
     5641 aggtccgcaa gacaacggaa aagaaagctg aatgagtatt cgcataatcc cgcaagatga
     5701 gctgggttcg agcgagaaac gtacggcgaa tatgattccg ccgttattgt tccctcggct
     5761 caagaattta tacaaccgcc gcgccgagcg tctgcgcgag ctggcagaaa ataatccgct
     5821 gggtgattac ctgcgctttg ctgcgcttat cgcccacgcc caggaagtgg tgctgtacga
     5881 ccatccgctg gagatggatc tgaccgcgcg cattaaagaa gccagcgcac agggcaagcc
     5941 cccgctggat attcacgttc tgccgcgtga taagcactgg caaaagctgc tgatggcgct
     6001 aattgctgag ctgaaacctg aaatgagcgg cccggcgctg gcagtgattg agaatctgga
     6061 gaaggcatcg actcaggagc tggaagatat ggccagcgcg ctgtttgcct ctgatttctc
     6121 gtccgtcagc agcgataaag cgccgtttat ctgggctgcg ctgtcgctct actgggcgca
     6181 gatggccaat ctgatccccg gcaaagcccg cgctgaatac ggcgaacaac gtcaatattg
     6241 cccggtatgt ggctctatgc cggtgtccag catggtgcaa attggcacca ctcagggtct
     6301 gcgttacctg cactgcaacc tgtgtgaaac cgaatggcac gtagtgcgcg taaaatgcag
     6361 caactgcgag cagagcggca aactgcatta ctggtcgctg gatgacgaac aggccgcgat
     6421 taaagccgaa agctgcgatg actgcggcac ttacctgaag attctttatc aggaaaaaga
     6481 accgaaagtt gaagccgtgg cagatgacct cgcctctctg gtactggacg cgcgaatgga
     6541 gcaagaaggc tacgcccgta gttccattaa cccgttcctg tttccgggtg aaggagagta
     6601 atctctgacg ttttccgggt ggcgcaggtc gcccggatta cagctcatag ggtgtgacga
     6661 caccatctct cgtattccgc gttacccatt caagatcatg ccgcaattgt ctgtttgcct
     6721 cgttcttccc tccaacacat cgataaggtt atgggtatgt cactgatgac aagcgtatga
     6781 ttgcctgatg cgctgcgctt atcaggccta ccaataaact tcaatatatt gaatttgcat
     6841 gactttgtag gccggataag gcgttcacgc cgcatccggc atgaacaacg cgcacgttgt
     6901 cagcaatctg attgacatca ttttttccca ttaccattcc tcgcgcagct tacgctgata
     6961 ttcgacacca tcttcctcac acccttgcca gatgccagga acactggttc ttgctgtttg
     7021 cgattgattt atcaggtatt gatctaccgc ttcccttaat aggtccgcac gaggaagatt
     7081 acgctgcacc tcgagatcat caagttgctt aatcacctca ttcgataaat cgagtaaaat
     7141 tctgcccata tctatacctg ccagagagtt cataaatatc tcgccaatat catttttgaa
     7201 tctatggaga gaaaagtacc cttgccgaat ctttaaagaa agctcattta cgcatcattt
     7261 tttatttttg cgaggcgttt tccagatacg atgtaacagt tgcagttatt ataagttcaa
     7321 gtaaaacagc cttgtattaa taccagaata agggcatctg ttttttatat tcaagaatga
     7381 aaaatttttt tcattcctta tgctccttac actgatcatc aacaccaact acctccccct
     7441 tttgcctgcc tccctttgct ttacgcaccg gaaattgctg ccgctccaga tattgctcaa
     7501 ccgcttcgcg taaaattgca gatcgtgaaa ccttacgcca gccccgaagc ttgttcagcc
     7561 gtttaattgc ctcttctgat aaatgaagaa aaaccgtatt catcgccatt accaccacct
     7621 tacatatatt actcattaat gtatgtgcga aaattacgcc gaaaaagcat ttaaaaaaat
     7681 gcggattttt gcatgtcatt ttgtttttgc gaggcgtttt ccagaaataa taagagcgtt
     7741 gcagttgttt catttattac tcaaaaatgg tttagttatc gcccaggatg agttatgggt
     7801 gctatgaata tattatattc acggatattt atttgcataa tctgaatggt gtagccgaaa
     7861 aacgtcagca tcctaacagc accaggattt aaggtgaaat taatctttca tcacagaccg
     7921 gtcagtactg ccaccaaccg gtctttactt gctcatcgtt ataattttgt tggtaacagt
     7981 tcgggaaaca aggaattcct tccggaacac ccccggcatc atccgatagc attgacgtca
     8041 atcatcagcg cgacgatgag tttttttaat cgcatcatag ggtggcgacg ttcagcacga
     8101 ttacgggtca tagcaaaact cctgagcagg cggccactat gccgctttca ctggagttct
     8161 gaatactggt actttctgta tggcttcgcc atgcccctcg cggctaagta cctgcacaca
     8221 tctctctttg tatgtcattt ttgcaatgca ttgtccgaat tctgataatc ctaatgagtt
     8281 ttactcgtca gactcccatc cctacggtat acgagttata ttcatgaatg aaatcgacat
     8341 ttttaaaaaa cgaatcttgt aaaacatcag tgttgatatt accttgcaat ccaacattga
     8401 atttgttaga gacatgaaga actcgaatat caaaaaggtt ttttaacatc attaattctt
     8461 cgggtgttaa tgaatcgcaa ctaaaatatc cctgttgatc aaaaagattt tctatttctc
     8521 cactatcggc gaggcgtttt tcaaggagtg tttgcaacat cgagttgcca aggacaatgg
     8581 atatcgtgcg atctttcgca gatttacacc ccatcacaac ggcaacattc gtatccccct
     8641 caagagtttc atttatcgcc gtcgctaaaa tggtggtcaa acacgcaaac ggaactgtat
     8701 ttctttcgtg atttaattta tctaccagtt cattccattt atcaaaagcc gggctatatt
     8761 tgttgcgcac agaaatatcc gactggttaa acaattcagc catgcgattt tttatcatct
     8821 cgatatccgg cgaaccagaa agactttgcg cataatacgg tcctgatgat gttgcataca
     8881 ttaatgccaa gcccacaact gtcgccatga tgggcgcagc cacggttttg gcaagggcga
     8941 cgctgcgatg gctatgcgct tcacaggcta aaggaacata ttccactgac tgcggtaatt
     9001 ccttacatgc ctccctcatc attgcggtat gctttacaaa caacggtttt tcactgccca
     9061 aaaaaacttt ctgcttaatt gcactaagaa aacgcaaatc aaaaataatt gtttttggtt
     9121 tattattttt tggcacattt cctattatcc cgctaagaac cttccttgct gcatggagat
     9181 ttttttgcac agcacataac acaattgatg aagccttctc cactgcatta ttgattttaa
     9241 ttttaaaatc cgcatgatat acgttgacgg gcaatgcaag tatatctgga cctacatctt
     9301 ttgctgagta tttattggtg atcgacggac gtgaacccac gagaaaactt agctgcaaat
     9361 tatgctctat attatcagct gtttttgtgg tgtggttaaa catagagatg ccacctttca
     9421 ttctttcaca catatcctca actttttcac ttcgttcttg tggcaatttg gctttaacat
     9481 taatttgatg atttttaccc gtgcattcct taactacaag gggaatgtta tctaaagtgc
     9541 catacaggct aataacgtca ccaggatttt tatcatgtaa gtaagtttga ttaatacttg
     9601 aagcattcac aaagagggtc aatattataa ctgtttataa cttcacctac atcaagtgtg
     9661 tagtgcttac tctggagact gccatcctta tttaattgta tatcaatcaa tatatccccg
     9721 gactttgtca ccatatcttt gccataaacc atttgcatag ccatttcgag gagattttta
     9781 tctaactgat tgcgggcgtt gagtaagcga ttaccaaata atttatatac tgttcccgtc
     9841 attgccagcg ctgcgctttc tggtactttg cgtttccaca gtgaggtttc tctattttta
     9901 aacaggtcat attcattatt actcgacaaa ccatgcttca gttcgttaac acaatcgcac
     9961 agttgctcct caccttccat tttaaggcaa agtgaaaatt gatttaatgg cgtttacttc
    10021 ggtgatttat tactcattag ttgtttttct aaattaacaa cagaataact ttcttgttta
    10081 atgagattaa tattggcaac cattatagcc tccttcccac ggtttaagag caaacataca
    10141 acataatgat tcataacata cagagcagta aatgaatgat actgacaatg tatgccagcc
    10201 cattatcttt gcataatgtt tgtgagctat agattttaaa aatatttatg aaaaaagtat
    10261 ggcaccagag ggggttttga ctatcaaaaa tcgctcaaac ctgaactaag atgatgagtt
    10321 aagcattatc tgcaataatt gccactcagc tctacaaata atatttaaat gctcctgaac
    10381 agtgatgtag caagacacgt atggtgccaa cgaagaataa aaatttataa agttcttatt
    10441 caatataacc ggaatagtca gggactgatg taaatgaccg gaatgaatga gagaggcagc
    10501 accgcccctc tccataagct actcttcttc gttcccgccc tcttcatacg ggccaaatgg
    10561 cttcgcgggc agaacgatat acgtgccttc aaacactgca cccggcgtct cgtcgccaaa
    10621 gatttcgacc tgcatctgca cccgtgcttt tcgtccgcgc gccagacggt cgagatcgcc
    10681 gcttaaggca ccaaggtcgg ctaccgcatg gggtttaccg ctaatcggtt tgctgtagcg
    10741 gatatgcgca tccgccagaa taatcgttcc gccgaggtgg cgttcacgca gcatcagcca
    10801 gataagcccc caaccggtga gcgtcgccag cgagaataaa ctcccggcaa acagcgtatg
    10861 gtgcggattc tgattgccgg tttccggcat ggtggtgata aatttttgcc cggtatattg
    10921 ttgaatacgc acgcccattt tttcactaag cgggatatgt tcgtaccacg cctgttgcag
    10981 ctgcgcgcac cagtcgccgc gatgcaaaat atcatccaga gtggcgacgg gcttaatcat
    11041 caaaaaatgg cgaatcggcg tggtggttgg cgtggtgatt tctccctgat taacaaaccc
    11101 cagcttggcg aaaaactcca ccgcgtcttc acgggcgcta caggtcacgc gcttaacgcc
    11161 ttcctgacgc gccaccgact ccagggtcat cgccatcagc gttcctaacc ctttgtcctg
    11221 cacgtccggg tgaacggcca taaagcgaat ggacgcttca ttgtcggcat taatatacaa
    11281 tcggcctacc gccaccagat taccctgctc gtcgacgacc atctggtgat gcgccatcgc
    11341 atcccacgcg tcgcgttccg aaccttttgg ttgatgcagg ggcttacgca acatttccca
    11401 gcgaaactga tagtaacgct ctaactcttc ttctgtttgt ggaacctgaa ggtgatacat
    11461 agcgatactc tctcttgttt cccgtgacaa ccctggaagc tggctcatac ctgcaaccag
    11521 aatgtcacgg ggccatcatt gaccagcgat acctgcatat cggcagcgaa gcgtcctgtt
    11581 tgcgtgttca tctcttgctg gcggcagcgt tcgacgaaat agtcatataa cgcctctgcg
    11641 cgatccggtg atgcgccttt ggagaaactt gggcgcatcc cccgttcggt atctgcggcg
    11701 agggtaaact gggaaaccac cagtacactg ccgcccgcct gttgcacgtt gagattcatc
    11761 ttgccttcgg catcgctaaa gatgcggtag ccgagcacac gctcgcacag acggtttgct
    11821 ttctgttcgt cgtcatcctt ttcgacaccc aataacacca aaagtcccgc gccaatttcg
    11881 cccgtcactt ctccctccac ggtgacgctg gcacgggtta cgcgttgaat taatgcaatc
    11941 atggttcgtc gtcttcttct tgttcagctg cttgttttag tttgcggtat tccccgagag
    12001 tgacagtaat ttccgcgcca agcaagacga tacaccatgt ccagtagacc caaacaaaga
    12061 gaatggggat caccgccagc acaccgtaaa tgagctgata tgacgggaac atggtgatat
    12121 aaagcgcgaa acctttcttt cctgcttcga acaggagtgc ggcgacaaac gcgccgacaa
    12181 tcgcgtcgcg gttaggtacg cggatggtag gaacaatgct gtacagcaac cagaaggaga
    12241 tccacgacaa cagcaacgga aaaatacgca gcacgttgtc gatgacagta ttgagatcgc
    12301 tcgcccagcg cagggagagc aaataggaac tgatcgccag actggcccct gccagcagcg
    12361 gccccagcgt taaaatcatc cagtacacgg cgaacgagta aatttttggt cgcgctcgtt
    12421 tactgcgcca gatggtattc aacgcgctat cgatggagta catcaacaat aacgcggtga
    12481 cgatcagccc gcacgcccca acggcggtca tcttgttgga attagcaaca aattgttcga
    12541 tataccgctg aataacatcg ccagtagcag gcagaaagtt ggcaaaaata aagtgacgta
    12601 actgaatgct gacgtcggaa aacatgggaa aagcggcgaa aagcgcaaaa acaacggcaa
    12661 ccagcggcac taatgagagc aacgacacat aggcaaggtt acctgccagg gttgtcatgt
    12721 tgtcctcatc aatacgttgc cagagtagtt ttagccaggc ccatagtgga cgggtacggt
    12781 gcctggcttt gtcctgaatg gtttttagca taacaccttc gcgaaatagt ccgggatggt
    12841 ggttttatct ttcaccagaa tactggtaat gcctagctga ttagctcctt ctatattatc
    12901 ggcgttatcg tcgaaaaaga ccgtatcgct gggtgaaaaa ccttccgcct gcaaaacatg
    12961 ctggtaaatt cgtgcttcag gtttgcgcat ccccagatct tgcgacagat agatatggtc
    13021 agcagcatca cgaatttccg ggtattcttc cggccagaag gtggtatgca ggcggttggt
    13081 attggaaagc accaccacgc gatgcccctg ctcacgcagt ttatgcatga tggcgatcac
    13141 ttccgggcgc agcgcaacaa acaccgcctg ccagccgtga gagaactgct cgtagcttag
    13201 cggtagagcc atctcatgac acagcgcctc tgcgaacgct tcgtcgctaa tttccccacg
    13261 ctcatgctga tgaaacgcct cccccatatg aaaactcttc ttaagcgatg ccagcggaat
    13321 acgcgttaaa tcgctccagg ctcccagcac acggttaaag tcgatatcga caatcacatt
    13381 acctaaatca aagatataga gcatttttgc ctcctttcgc gccacaagaa attaactgta
    13441 gcgggaaagg atgggtttgg ctatgtggca aggtatgaag ccaggttaac ggtcacaaaa
    13501 atctacgcgt cttcctgggg aagaatgatt tcaatttggc tgtcttgtaa gcgtgatttt
    13561 agcgcagcgg gcggcagctc gtcggtaaag agcgcagtga cctgtgccac gttaccaatt
    13621 tcaaccgccg ccgaagcatg atacttagtg tgatcggcga ccagcagaat atttctcgcg
    13681 tgcgccatca tcgttttcac cacgttagct tcgtttacat caaactccat caacgcgcca
    13741 tcgctctcaa tcgccccaac gcttgttacc agataatcag cacgaaaatc agccacaaag
    13801 gacgccgctg aagggccaat gatcccgcta ttatgagagc gcaacgtacc gccgggcacc
    13861 atcacttcaa agcgcgggtt gtggtaaaga atatgcgcca cacgcaggct gttggtgatt
    13921 atccgcaaat gattatggtt aagtaacgcc cgggcaacat gctcaacagt cgtaccaatg
    13981 gtgataaata ttgttgaacc atcaggaata tagtctgcca ccgcttcggc aatcgctttt
    14041 ttttctcggt ttgcgaaacc tcacgctgct cgaacgccgt attaacgacg ctggaagccc
    14101 gacccgcgcc accgtgatgg cgcgtaatca ggccttgctc gcttaattta cgaatatccc
    14161 ggcggaccgt ctgcgtggag acatccagca gatttgccag ctcatcaata ttcatatacc
    14221 cacgctcggc gatcagcatg aggagttggt cgtgccgcgg gttaccggtt agttcggtaa
    14281 ggctcatgaa aattcctcga aaaaccatca ccctggcatt ttatacaaaa agtgacaaaa
    14341 aagatcgggt ttgatcacag tcagggatcc cggctcgtcc accgggacgt gtgcatttta
    14401 acgccgctac accgctggcg aagcggactg actccgctaa atccccactt gttgccagcg
    14461 ccaccgccaa agcgccgtga aaaacatcac ccgcacctgt ggtatctacc acatcaactt
    14521 tgaaggccgg ttgatgctga cgcccaccat tttccagcca gtcgcagcct gcgctaccct
    14581 gggtcacata gacatgacca tttgtgagcg tttgtgcctg ttttagcgca ctggccatct
    14641 ctttcacgcc cgttaagcgc gccagacccg gttctgaaaa ggccgcgtga tcgcttaatg
    14701 ccaccagctc actgatatcc tgcggcgtaa tgtccccgtc cagaacggtc atcacacccg
    14761 cctgacgggc cagggtgaag gcttttttaa cgccgtcgtg ccagcgtaca tctgccagca
    14821 caacatccca ctgagagaaa tcaatttcct ccaaccactc tgcgtcaggc agcagatccg
    14881 ggctggggta gttaatgatt atccgctcgc ctttggtatc caccatgatg gcggattgcg
    14941 aagatttcgc ctggttatac cgtttggtgt aacgggtgtt aacgccccag gattccagct
    15001 ctgctagcag gctgttgccc gcgtcgtcat cacctacgcg accaataaaa tcgacctgcg
    15061 cccccagcct tgccgccgca accgctgccg tcgccgctgg cccgccacca acttccgtat
    15121 aatttctcgc cacgtattta ccgctctccg tcggtaaccc ttccacgtaa tagatgcgat
    15181 ccatcacggt tatacctaca caagcaacac gaatcatggt cattccttaa acattttggg
    15241 atgcgttcat tttcatttca ccaattgttt aaaaagtgac ggctgtcaat tttttgacga
    15301 taaattacaa atgcgatcaa aaacagacaa gcaaagcatg tcataaatga caaaaagtga
    15361 catcactgta ttcaggagag ggttatggca gcaatcgcgt ttatcggttt aggacaaatg
    15421 ggttcgccaa tggcgagcaa tttattgcag caagggcatc aacttcgcgt ctttgatgtg
    15481 aacgccgaag ctgtgcggca tctggtagac aaaggtgcga ctcccgccgc caacccggtg
    15541 caggccgcta aagatgccga atttatcatt accatgttgc cgaatggcga tctggtgcgc
    15601 aacgtgttgt tcggtgaaaa cggcgtttgc gaaggcttat ctaccgatgc gctggtcatt
    15661 gatatgtcca ccatccatcc gctgcaaacc gataaattga ttgccgatat gcaagccaaa
    15721 ggcttcagca tgatggatgt tccggtaggc cgtacttctg caaatgccat taccggtact
    15781 ctgttactgc tggctggcgg caccgctgaa caagttgaac gtgccacacc gatcctgatg
    15841 gcgatgggca gtgagttgat caacgctggc ggtccgggca tggggatccg cgttaagctc
    15901 atcaacaact acatgagcat cgcgctcaat gcgctttcgg cagaagccgc cgttttgtgc
    15961 gaagccctga atcttccctt cgatgttgcc gtcaaagtga tgagcggtac cgccgccggt
    16021 aaaggtcact tcaccacttc ctggccaaac aaagtcctca gcggagatct ttctcccgcc
    16081 ttcatgatcg atcttgccca taaggatctt ggcatcgccc ttgatgtcgc caaccagctg
    16141 catgtgccaa tgccgctggg ggccgcctca cgggaggttt atagccaggc gcgcgcatcg
    16201 ggcggttttg ccatgctggc ggtcgatcag cgcgaagcca tgcgcatgat gtttgccgcg
    16261 gctggggcac ccgctccggt agccgatagc gttttaactg atttcaaagt taacgctgca
    16321 aaggccctct cgccttatgc ctcggcgatt ctggtagatc aacaattctg ctaccgccag
    16381 gtggttgagc aaaacgcgat tgccaaaagt tgcgccatga ttgtcgccgc cgatgagttc
    16441 attcctggca acggtattcc ggtcgatagc gtggttattg accgcaaaat caatccgcta
    16501 cagatcaaac aagacggtgg caaagcctta aaactgctgg tgctgtggcg tagtgatgaa
    16561 gatgcgcagc aacgtctgga tatggtgaaa gagttcaacg gactgtgcca ctcacacggt
    16621 ctggtaagca tcattgagcc agtcgtccgc ccaccgcgtc gtggcgataa attcgatcgc
    16681 gaacaagcgg tcatcgatgc cgccaaagag ctgggcgaca gtggcgctga cctctacaaa
    16741 gttgaaatgc cgctttatgg caaaggtccg caacaagagc ttctctgtgc ttcacaacgt
    16801 ctgaatgacc atatcaatat gccatgggtg atcctctctt ccagtgtcga cgaaaaactg
    16861 ttcccgcgtg ccgtacgcgt ggcaatgacg gcgggcgcat cgggattcct ggcaggccgt
    16921 gcagtctggg catcggtcgt cggtttacca gacaacgagc tgatgctccg tgacgtttgc
    16981 gcaccgaaat tacaacaact tggcgatatc gtcgacgaaa tgatggctaa acgccgctaa
    17041 gatgaggata aaaggaatga aatggtttaa caccctaagc cacaaccgtt ggctggaaca
    17101 ggaaaccgac cgcatctttg attttggtaa aaattccgta gtgccgactg gttttggctg
    17161 gttaggcaat aaagggcaaa tcaaagaaga gatgggcacc catctgtgga tcacagctcg
    17221 tatgttgcac gtttattccg ttgctgcggc gatgggtcga cctggcgctt acgcgttggt
    17281 tgatcacggt attaaagcca tgaacggcgc actgcgtgat aaaaaatatg gcggctggta
    17341 tgcctgcgtg aatgacgagg gcgtggtgga tgcctccaaa cagggctatc aacacttctt
    17401 tgctctgctg ggtgccgcca gcgccgtcac aacgggtcac ccggaagcgc gcaagctgct
    17461 cgattacacc attgagatta tcgagaaata tttctggagc gaagaagagc agatgtgcct
    17521 ggaatcctgg gacgaagcct tcagcaaaac cgaagagtac cgcggcggca atgccaatat
    17581 gcacgcggtg gaagctttct tgattgttta tgacgtcact cacgacaaaa aatggctgga
    17641 tcgcgcgatt cgcgtggctt ccgtgattat ccacgacgtc gccagaaata atcattatcg
    17701 cgttaacgaa cacttcgata cccagtggga tccgctgccg gattacaaca aagataaccc
    17761 ggcgcatcgc ttccgcgcgt tcggaggtac accaggccac tggatcgaat ggggccgttt
    17821 aatgctgcac atccatgctg ccctggaagc ccgttgcgaa caaccaccag catggctgct
    17881 agaagatgcc aaaggtctgt ttaacgccac cgtgcgcgat gcctgggcac ccgatggcgc
    17941 ggacgggatt gtttataccg ttgactggga aggaaaaccg gtggtccgcg aacgtgtacg
    18001 ttggcctatc gtcgaagcaa tgggtacggc ctacgcgctc tacaccgtca ctggcgatcg
    18061 ccagtatgaa acctggtatc aaacatggtg ggagtactgc attaagtacc tgatggacta
    18121 tgaaaatggt tcctggtggc aggagctgga tgcggacaat aaggtcatca ccaaagtctg
    18181 ggacggcaaa caggatattt atcacctgct gcattgcctg gtgatcccgc gtatcccgtt
    18241 agcgcccggc atggctccag cggttgcagc gggtctgctg gatattaatg cgaaataact
    18301 gatctgaaaa tagggtgcgg gttttcccct caccctaacc ctctccccaa aggggcgagg
    18361 ggaccgtccg gtgcagtttt tgggtttcac atcagcctca cgggaggctg tatgtcgtta
    18421 attaaggtgc cttgtatgca aatcaccaat atgcattgca gtggacagac cgtttcactt
    18481 gccgcggggg attatcacgc gaccattgtg accgtcggtg ctggtctggc tgaattaacc
    18541 tttcagggtt gtcatttagt catcccgcac aaacccgaag agatgccgct ggcgcatctg
    18601 ggtaaagtgc ttattccgtg gccgaaccgg atcgccaacg gctgttaccg ttatcagggg
    18661 caggaatatc agttacccat taatgaacat agttcaaaag ccgctattca cgggctgctt
    18721 gcctggcgag actggcaaat tagtgaatta accaccacca gcgttaccct gacagctttt
    18781 ctgccgccca gttacggcta tccgtttatg ctggcttcac aagttgttta ttcgctcaac
    18841 gcacacacgg gtctttccgt tgagatcgca agccagaata tcggcactgt cgcagcgcct
    18901 tatggcgtgg gtattcatcc ttatttaacc tgcaacttaa catctgttga tgaatatctg
    18961 ttccagcttc cggcaaacca ggtttatgcc gtcgatgaac acgtaaatcc gacaacgctt
    19021 caccacgttg atgagctgga tttaaatttc acgcaggcga aaaaaatagc cgcgacaaaa
    19081 atcgaccaca cctttaaaac cgctaatgat ttgtgggaaa tgaccattac ccatccacag
    19141 caggcgcttt cggtaagcct ttgttccgat caaccctggg tgcaggttta tagcggtgaa
    19201 aaattacagc gtcagggact ggcagttgaa cctatgagtt gcccgcctaa cgcctttaat
    19261 tcaggtatcg acttattatt actggaacca ggaaaaccgc accgcctgtt ttttaatatt
    19321 tacggacaga gaaaataact tgtattgatt agcagtcaaa atcattgaag aacatttcac
    19381 tcgctaaagg caacattatg tatcttcaat ttattaagtt aaaaacagct cagcttctgt
    19441 aaagttttta tatatcagtt ttatgagaat cattttacat aagcctgtaa taacaggaag
    19501 gcaacaatct cgttctcaac tctatggaga ttagttatgg atacgccacg tccacagtta
    19561 ttagattttc aatttcatca gaataacgac agttttaccc tacattttca acaacgtctt
    19621 attttaaccc atagcaaaga taatccttgt ttatggattg gctcaggtat agcggatatc
    19681 gatatgttcc gcggtaattt cagcattaaa gataaactac aggagaaaat tgcgcttacc
    19741 gacgccatcg tcagccagtc accggatggt tggttaattc atttcagccg tggttctgac
    19801 attagcgcca cgctgaatat ctctgccgac gatcagggcc gtttattgct ggaactacaa
    19861 aacgacaacc ttaaccacaa ccgtatctgg ctgcgccttg ccgctcaacc agaggaccat
    19921 atctacggct gcggcgaaca gttttcctac ttcgatctgc gcggcaaacc gttcccgcta
    19981 tggaccagtg aacaaggcgt tggtcgcaac aaacaaacct atgtcacctg gcaggccgac
    20041 tgcaaagaaa atgcgggcgg cgactattac tggactttct tcccacagcc tacgtttgtc
    20101 agcacgcaga agtattactg ccatgttgat aacagttgct acatgaactt cgactttagc
    20161 gccccggaat accatgaact ggcgctgtgg gaagacaaag ctacgctacg ttttgaatgt
    20221 gctgacacat acatctccct gctggaaaaa ttaactgccc tgctgggacg ccagccagaa
    20281 ctgcccgact ggatttatga cggggtaacg ctcggcattc atggcgggac ggaagtgtgc
    20341 cagaagaaac tggacaccat gcgtaacgcg ggcgtgaagg ttaacggcat ctgggcgcag
    20401 gactggtctg ggatccgtat aacctctttt ggcaagcgcg tgatgtggaa ctggaagtgg
    20461 aacagcgaaa actacccgca actggattca cgcattaagc agtggaatca ggagggcgtg
    20521 cagttcctgg cctatatcaa cccgtatgtt gccagcgata aagatctctg cgaagaagcg
    20581 gcacaacacg gctatctagc aaaagatgcc tctggcggtg actatctggt ggagtttggc
    20641 gagttttacg gcggcgttgt cgatctcact aatccagaag cctacgcctg gttcaaggaa
    20701 gtgatcaaaa agaacatgat tgaactcggc tgcggcggct ggatggctga cttcggcgag
    20761 tatctgccca ccgacacgta cctgcataac ggcgtcagtg ccgaaattat gcataacgcc
    20821 tagcctgcgc tgtgggcgaa gtgtaactac gaagcccttg aagaaacagg caagctcggc
    20881 gagatcctct tctttatgcg cgccggttct accggtagcc agaaatactc caccatgatg
    20941 tgggcgggcg accagaacgt ggactggagt ctcgacgatg gcctggcgtc cgtagtcccg
    21001 gcggcgctgt cgctggcaat gaccggacat ggtctgcatc acagcgacat tggcggttac
    21061 accaccctgt ttgagatgaa gcgcagcaaa gagctgctgc tgcgctggtg cgatttcagc
    21121 gccttcacgc cgatgatgcg cacccacgaa ggtaaccgtc ctggcgacaa ctggcagttt
    21181 gacggcgatg cggaaactat cgcccatttt gcccgtatga ctaccgtttt caccaccctg
    21241 aaaccttatc tgaaagaggc cgtcgcgctg aatgcgaagt ccggtctgcc ggtcatgcgc
    21301 ccgctgttcc tgcattacga agacgatgca catacctatt ccctgaaata tcagtacctg
    21361 ttaggccgcg acattctggt cgctccggta cacgaagaag gtcgcagcga ctggacgctc
    21421 tatctgccgg aggataactg ggtacatgcc tggacgggtg aagcgttccg gggcggggaa
    21481 gttaccgtta atgcgcccat cggcaagccg ccggtctttt atcgcgccga tagcgaatgg
    21541 gcggcactgt tcgcgtcgtt aaacagcatc taatcacatc cgcccgtggg gaaactcatg
    21601 ggcaaaggga gaacatcgat gagtcacatc acaacggaag atccagcaac tttacgcctg
    21661 ccctttaaag agaaactctc ttacggtatt ggcgacctgg cctctaacat cctgctggat
    21721 atcggtacgc tttatctttt gaagttttat accgacgttc tggggctgcc aggcacctat
    21781 ggcggcatta tctttttgat ttcaaaattc tttactgcgt ttaccgatat gggtaccggc
    21841 attatgttgg attcccgacg caagatcggt ccaaaaggta agttccgtcc ttttattctg
    21901 tatgcgtcat tcccggtcac cttactggcg atcgccaact ttgtcggcac accgtttgat
    21961 gtcaccggta aaacggtgat ggccactatt ctgtttatgc tttacggact gtttttcagc
    22021 atgatgaact gctcctacgg cgcaatggtc ccggcaatca ccaaaaaccc caacgaacgc
    22081 gcctcactgg cggcatggcg tcagggcggc gctacgctgg gcctgctgct gtgcacggtg
    22141 ggattcgtgc cagttatgaa tcttatcgaa ggtaatcaga aacttggcta tatcttcgcc
    22201 gccacgctgt tttcactgtt cggcctgctg tttatgtgga tctgctactc aggcgtgaaa
    22261 gagcgttatg tcgaaaccca accaaccaat ccggcgcaaa agcctggcct gttgcaatct
    22321 ttccgcgcaa ttgccggtaa ccgcccactg ttcattctgt gtattgccaa cctctgcacc
    22381 ttaggggcgt ttaacgtcaa gctcgccatt caggtctatt acacccagta cgtacttaac
    22441 gatcccatcc tgttgtcgta tatgggattt ttcagcatgg gctgtatttt catcggcgtg
    22501 ttcctgatgc ctggcgcagt cagacgtttt ggtaagaaga aggtctatat cggcggcctg
    22561 ctgatttggg tgctgggcga tctgctcaac tatttcttcg gcggcggctc ggtcagcttt
    22621 gtggcgttct cctgcctggc gttcttcggc tcagcgtttg ttaacagcct gaactgggcg
    22681 ctggtttccg acaccgtcga gtacggcgag tggcgcaccg gcgtgcgttc ggaaggcacg
    22741 gtctacaccg gcttcacctt ctttcgcaaa gtgtctcagg cgctggctgg tttcttcccc
    22801 ggctggatgc tgacgcaaat tggctatgtg ccgaacgtcg cacaggctga ccacaccatt
    22861 gaagggttac gccagttgat cttcatctac ccaagcgcac tggcggtagt caccattgtg
    22921 gcgatgggtt gcttctacag cctgaacgag aagatgtatg tccgcattgt ggaagagata
    22981 gaagcccgta aacgcacggc gtaataataa taaacgacgc cctgcggggc gttataagga
    23041 gtgattatgt ctgaccataa tccactgaca ttaaaactga atctgcggga aaaaatcgcc
    23101 tatggtatgg gcgacgtcgg ttcgaattta atgctctgca tcggtactct gtatctcctc
    23161 aaactttata ccgatgaatt agggatgcct gcttactatg gcgggattat ctttctggtc
    23221 gcgaagtttt ttaccgcgtt taccgatatg ctcaccggat ttttactcga ctcgcggaaa
    23281 aatattgggc ctaaaggcaa atttcgtccc tttattttat atgcagcagt tccggcggcg
    23341 ttaattgcaa cgctccagtt tatcgccacc accttttgtt tgccggttaa aacgacgatt
    23401 gccaccgcgc tgtttatgat gtttggactt tcatacagtc tgatgaactg ctcgtatggt
    23461 gcgatgatcc cggcaattac caaaaacccg aatgagcgcg cgcagctcgc ggcttaccgc
    23521 cagggtggtg caaccatagg gctattgatt tgtaccgtgg cgtttattcc gttgcagtcg
    23581 cttttttctg actcaaccgt cggttatgcc tgtgcggcac ttatgttctc cattggcggc
    23641 tttattttta tgatgctgtg ctacagaggc gtcaaagagc attatgtgga cacagcgcca
    23701 accggacata aagccagtat tctcaaatct ttttgcgcga tatttcgtaa tccaccattg
    23761 ctggttttat gcattgctaa cctgtgtact ctggcggcat ttaatatcaa actggcgatt
    23821 caggtctatt acacccagta tgtgctgaat gatattaatt tattgtcgtg gatgggattc
    23881 ttcagtatgg gatgcatcct cgtcggcgta ttactggtgc cagtaactgt aaaatgtttt
    23941 ggtaaaaaac aggtttattt agctggcatg gtgctatggg cggtgggtga tatactgaat
    24001 tatttctggg gaagtaactc tttcactttc gtcatgttct cttgtgtcgc cttttttggc
    24061 acggcctttg taaacagcct gaactgggca ctggtaccag ataccgtaga ttacggtgaa
    24121 tggaaaaccg gtattcgtgc cgaaggttct gtttataccg gttatacctt ctttcgtaaa
    24181 atttctgccg cacttgctgg cttcttgcca ggcattatgc tgacgcaaat tggttatgtt
    24241 cccaacatcg cgcaaagcga tgcgacatta cagggtttgc gtcagctcat ttttatctgg
    24301 ccttgtgcac tggcaattat tgctgcattg acgatgggat tcttttacac actcaatgaa
    24361 aaacggtttg cattgattat tgaggaaatc aaccaacgaa aaaataaaga gatggcgaca
    24421 gaaaaaaaaa cggcttccgt aacgttataa atgtcaatag ccgctatttc catcctggtg
    24481 ggtggcggcc tccctacgtt taaaaaatgg acttattatg aaaaagatta atgcaataat
    24541 tttattatct tctctgacct ctgcctcggt atttgctggc gcatacgtcg aaaacaggga
    24601 ggcatataat cttgcctccg atcagggcga ggttatgcta cgtgtgggtt ataacttcga
    24661 tatgggcgcg ggtattatgt taaccaatac ctacaccttt cagcgagaag atgaactaaa
    24721 acatggctat aatgaaattg aaggttggta tcctttattc aaaccaaccg ataaattaac
    24781 catccagccc ggtggctt
//
LOCUS       DADMGC010000054        24401 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.054, whole genome shotgun sequence.
ACCESSION   DADMGC010000054 DADMGC010000000
VERSION     DADMGC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.054"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..373
                     /locus_tag="JNZ73_002112"
     CDS             <1..373
                     /locus_tag="JNZ73_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8732396.1"
                     /translation="IAGYYMKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQIL
                     NAIFLGILCGIGMLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHAL
                     FWLAIGMLGIAMICLLFIKDI"
     gene            484..1407
                     /locus_tag="JNZ73_002113"
     CDS             484..1407
                     /locus_tag="JNZ73_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY8732397.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(1411..2229)
                     /gene="nlpA"
                     /locus_tag="JNZ73_002114"
     CDS             complement(1411..2229)
                     /gene="nlpA"
                     /locus_tag="JNZ73_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HAY8732398.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVQLVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            2451..2744
                     /locus_tag="JNZ73_002115"
     CDS             2451..2744
                     /locus_tag="JNZ73_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732399.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2785..3975)
                     /gene="nepI"
                     /locus_tag="JNZ73_002116"
     CDS             complement(2785..3975)
                     /gene="nepI"
                     /locus_tag="JNZ73_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HAY8732400.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4186..4638)
                     /locus_tag="JNZ73_002117"
     CDS             complement(4186..4638)
                     /locus_tag="JNZ73_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HAY8732401.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(4691..6025)
                     /locus_tag="JNZ73_002118"
     CDS             complement(4691..6025)
                     /locus_tag="JNZ73_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY8732402.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVVLAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6200..7966
                     /gene="adeD"
                     /locus_tag="JNZ73_002119"
     CDS             6200..7966
                     /gene="adeD"
                     /locus_tag="JNZ73_002119"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAY8732403.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPAVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(8012..9403)
                     /gene="uhpT"
                     /locus_tag="JNZ73_002120"
     CDS             complement(8012..9403)
                     /gene="uhpT"
                     /locus_tag="JNZ73_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HAY8732404.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEVSEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(9541..10860)
                     /gene="uhpC"
                     /locus_tag="JNZ73_002121"
     CDS             complement(9541..10860)
                     /gene="uhpC"
                     /locus_tag="JNZ73_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HAY8732405.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(10870..12372)
                     /gene="uhpB"
                     /locus_tag="JNZ73_002122"
     CDS             complement(10870..12372)
                     /gene="uhpB"
                     /locus_tag="JNZ73_002122"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HAY8732406.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(12372..12962)
                     /gene="uhpA"
                     /locus_tag="JNZ73_002123"
     CDS             complement(12372..12962)
                     /gene="uhpA"
                     /locus_tag="JNZ73_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HAY8732407.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(13037..13327)
                     /locus_tag="JNZ73_002124"
     CDS             complement(13037..13327)
                     /locus_tag="JNZ73_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase isozyme 1 small subunit"
                     /protein_id="HAY8732408.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13331..15019)
                     /gene="ilvB"
                     /locus_tag="JNZ73_002125"
     CDS             complement(13331..15019)
                     /gene="ilvB"
                     /locus_tag="JNZ73_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HAY8732409.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPSFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQLRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(15125..15223)
                     /gene="ivbL"
                     /locus_tag="JNZ73_002126"
     CDS             complement(15125..15223)
                     /gene="ivbL"
                     /locus_tag="JNZ73_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HAY8732410.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            15788..15877
                     /gene="tisB"
                     /locus_tag="JNZ73_002127"
     CDS             15788..15877
                     /gene="tisB"
                     /locus_tag="JNZ73_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HAY8732411.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            16157..17341
                     /gene="emrD"
                     /locus_tag="JNZ73_002128"
     CDS             16157..17341
                     /gene="emrD"
                     /locus_tag="JNZ73_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HAY8732412.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYKRTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(17349..17846)
                     /locus_tag="JNZ73_002129"
     CDS             complement(17349..17846)
                     /locus_tag="JNZ73_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAY8732413.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(17843..18205)
                     /locus_tag="JNZ73_002130"
     CDS             complement(17843..18205)
                     /locus_tag="JNZ73_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAY8732414.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(18195..18542)
                     /locus_tag="JNZ73_002131"
     CDS             complement(18195..18542)
                     /locus_tag="JNZ73_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HAY8732415.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLTWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            18651..19064
                     /locus_tag="JNZ73_002132"
     CDS             18651..19064
                     /locus_tag="JNZ73_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732416.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(19111..20494)
                     /locus_tag="JNZ73_002133"
                     /pseudo
     CDS             complement(19111..20494)
                     /locus_tag="JNZ73_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(20529..22244)
                     /locus_tag="JNZ73_002134"
                     /pseudo
     CDS             complement(20529..22244)
                     /locus_tag="JNZ73_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
     gene            22411..23304
                     /locus_tag="JNZ73_002135"
     CDS             22411..23304
                     /locus_tag="JNZ73_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8732417.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVLEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(23301..24115)
                     /locus_tag="JNZ73_002136"
                     /pseudo
     CDS             complement(23301..24115)
                     /locus_tag="JNZ73_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inactive 6-phospho-alpha-glucosidase"
     gene            complement(24115..>24401)
                     /locus_tag="JNZ73_002137"
     CDS             complement(24115..>24401)
                     /locus_tag="JNZ73_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HAY8732418.1"
                     /translation="KELDQAAGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEV
                     FKKLGAHGVFRSGDAIQVIIGLHVSQLREQLDSLINSHQSAENVAITEAV"
BASE COUNT         6141 a         6536 c         6072 g         5652 t
ORIGIN      
        1 aatcgcaggc tattacatga aacgtattgg caagcgacta ttaatgctca ttgctatcgt
       61 gagtggtatg tgtttttacg ccagcgtact catggcgacg actccggcgg ttgagctgga
      121 attgcaaatt ctaaatgcca tcttccttgg tattctctgt ggtatcggca tgctttattt
      181 tcaggacttg atgcctgaaa aaataggctc tgcgacaacg ttatatgcaa atacttcacg
      241 cgtcggctgg attatcgccg gctctgttga cggaattatg gttgaaatct ggagctacca
      301 tgcgttgttc tggctggcga tagggatgtt gggtattgcg atgatttgcc tgctgtttat
      361 taaagatatt tagctcacca gtctgggcta agaaaaatcg gcgtggcgca aacagagcca
      421 tcctcgccat ttttgctatc atgcctgcat acataaacga caaaacagta tgcagaggga
      481 aaaatgggtt ccaccagaaa ggggatgctg aacgttctga ttgccgccgt gttgtgggga
      541 agttcagggg tctgtgcgca atacatcatg gagcaaagcc agatgtcgtc gcagtttttg
      601 actatgacgc gtttgatatt cgccggtttg attctgctga cgctgtcatt tgttcatggc
      661 gataaaatct tttctattat taacaatcac aaagatgcca ttagtctgct gattttttcc
      721 gtggttggcg cgctaaccgt acagctcact tttttgctaa ccatcgaaaa atcgaacgca
      781 gccacggcaa cggtgctgca attcctctca ccgacgatta tcgtcgcctg gttctcactg
      841 gtacgtaaat cgcgcccggg cattctggtt ttctgcgcta ttttgacatc gctggtcggg
      901 acttttttat tggtgacaca cggtaatccg acgtcattat cgatctctcc tgccgcgtta
      961 ttctggggca ttgcctcggc atttgctgct gcattctata ccacctatcc ctcaacgctg
     1021 attgcccgct atggcacgtt gcctgtcgtc ggctggagta tgctgatcgg cggtctgatt
     1081 ctattgcctt tttatgccag acaagggaca aactttgtcg ttaacggcag tttgattttg
     1141 gcgttttttt atttgatcgt cattggtacg tccctgacat ttagtctgta cctgaaagga
     1201 gcacaattaa ttggcggtcc aaaagccagc attttgagct gtgcagaacc attaagtagc
     1261 gcgctactct ctttgctgtt gctggggatc acgttcacat taccggactg gctgggaacg
     1321 ctgctgattc tgtcatcggt aattttgatt tcaatggatt cccgtcgccg cgccagaaaa
     1381 ataaatcgtc cggcgcggga tgagtgagaa ttaccagcca ggcaccgcgc caccgttaaa
     1441 gatggtttct gccgctttag cgacttcgga tgattgataa gattgcagaa attccttcac
     1501 gttttctgca ttcttattat cttcccgtgc caccaaaata ttcacatacg gcgaattttt
     1561 atcttcaata aatacactgt cgtgcaccgg agaaagcccg gtctgctgaa tgtaagtggt
     1621 gctgataatc gccacatcaa ctttgggatc atccagtacg cgcgggagct gcgccccctc
     1681 gagttccata atctgcaaat gacgaggatt atcggttata tccagcgcgg taggtaataa
     1741 cccttttccc tctttcagag taattagctt ctctttttgc aacagtaaaa gcgcacgacc
     1801 aaggttggtc gggtcgttgg ggatcgccac tgtcgcgcct tcttttattt gcgccacggt
     1861 tttgattttt ttggaataac ccgccatggg gaagacaaac gtatttccca ccgctaccag
     1921 tttataacca tgcgcctgat tatcttgttc aagaaagggt cgatgctgga agacgttcgc
     1981 atcaagttcg ccatgatttg tcgcatcgtt cggtagcaac gaaccgctaa atcctaccag
     2041 ttgaacatcg aggccatatt tctcttttgc caccttttta gcgacttccg cgacatcttg
     2101 ttctgcgcca tttataacgc caactttaat gtgttttgca tcgctgctac tctggtcgca
     2161 acctgccagc agaattccgg ccagcaataa tgcggcccct gtccgtagat gatgtgttgt
     2221 cagtttcatt tttttatcct tttattaatg attacctgac tatagcggta aggtcgctgc
     2281 ggtttaaaaa acgaaacgct atcgataaga ataaaaagga ataaaagtgg aatataaagt
     2341 ctggcacttc ttactgacca cgcaagcgcg tttcgtacag catgatgaga gcgatgaaag
     2401 caaactacac ttatgcttca ttcgatacac atttgtaaaa ggttgaatcc atgaaaccaa
     2461 cgacactact gcttattttc acttttttcg ctatgccagg catcgtatac gccgagtcgc
     2521 cttttagctc actgcagtcc gcaaaagaga aaaccacggt attacaagat ttacgtaaaa
     2581 tttgcacgcc acaggcgtca ttatcagatg aagcgtggga aaagttaatg ttgtcagatg
     2641 aaaacaataa acaacatata cgcgaggcca tcgtggcgat ggaacgtaat aatcagagca
     2701 attattggga agcgttgggt aaggtagaat gcccggatat gtaagaacag caacaactac
     2761 tgaccaacgc ttctttacct gatatcagga tttcttcatt ttcacctttg cagtaaccaa
     2821 caatgcagtc agcaacatca atgtgccgga caacatcaac ggcgaagtca gaccaatatt
     2881 atccagcgca taaccgccga ttgccgcgcc acaggtatta gcaagctgaa taaccgccac
     2941 ctgaatagac ccggcttttt ctgcctgatc ggccagcgag cgggtgatcc acgttgacca
     3001 gccgacggga accaatgcaa aagttagccc ccagataatc gccacgccgg tagcaacgat
     3061 tttatcgctt ccccacaacg tcagtaccaa cgcactcaca gccagtatta acggcgcgcc
     3121 tgctaaggcc agttttaccg aacgtttaag aatgaacgac gaaagcgacg taccaataaa
     3181 gctggcgata ccaaaactca acagcaccag cgttaagcca tccacgccga atcccgccag
     3241 gttcatatac accgggcgaa tatacgtgaa gaaagcaaac tgcccggcga aagacatgaa
     3301 gatggcgatc atccctgcca tcacacccgg acgttgtaat aagcggaaag tattttgttt
     3361 ctgatgcgag ggttcgcctg gcagtgaagg caatgatttg ataatccaga aaatacacag
     3421 cacgcccatc accgccgccg cattaaagac attgcgccaa ccgataagct cgcctaaaaa
     3481 actgcccaac ggcgcggcaa tcaccagcgc aatagaaacc gcgccgaaga tcaccgacag
     3541 cgccttcggc accgtacgcg gcggtaccag acgcatggtc agcgacgccg acatcgccca
     3601 gaacccgccc agcgccagcc ccagacaggc acgaccgatt aaaagcaaac tgaatgagtt
     3661 agcaaaggaa accagcaagc aggagagcgt cagcaaaacg gcaaacaaaa taacaacgta
     3721 gcggcggtca gtggcctgaa ttgtctgggt aataaacaaa ctggcaaaca ttgccacaaa
     3781 ggcggtcacg gtcaccgatt gcccggcaac cccttccgaa atgcctaaat cctgggccat
     3841 tggcgtcaac aaactgacgg gcaaaaactc aacgataatc agacaggcga cacaaaacgc
     3901 caccgagaaa acggctgacc agttcggtcg ggtgatggca tccgcgccgc ggttttcggc
     3961 aataaattca ctcatggtgt tacccgtggc atgtttcagg ggaaaagccg accactttaa
     4021 cattgaaatg tgtgacgcat ttaacgtttt tgcaactttt acggtgttgc ttgagctgga
     4081 gttaagatcg aaacggaaca gggcaaagat taccggatgc ggcgtgaacg tcttatccac
     4141 ccgacaggtc aggcgcggtc tgtaggctga taagacgaaa cggtttcaat gcaaccagtg
     4201 aacgccctcg gcaggctgaa agaacgcgtt atagcgcatc tgaaaagcat taatatcctg
     4261 catttcaggt tcggtttcac gcagaaaacc cagcgccagc ctgacctgag catcggtaat
     4321 cggcgcggaa aggcgggctt tttgtaaaat ccggtgccat tgcagcaggt tctgttcgct
     4381 atcatcgaca atgacaattt gccagataag caacacctgc gcggcgtttt gcaggtgcga
     4441 ctcatccggg cgatgcaaat aacgtaaaaa ttcgtcaccg gctgactttc ccatttgatc
     4501 gaccatcgac tccacgggta cgacatcgat attcagccga tcgctgagac ggcgaatcgc
     4561 ttttctggcc ccggatgtca gcacccgaaa accaaccaca aacactaccg ccagcgtggc
     4621 gagcattatc cagatcatga gttctcctgc gtaatcagaa ctcatgatat ctggaaaccc
     4681 tcgccagtta ctaatccacc agaataatct tcaatgcgaa cagagctgcg accaccacca
     4741 cacacaggtt cagatcgcgc cagcgcccgg tgcacacttt catgatgcag tacgacataa
     4801 agccgagtgc aatcccttcg gtgatcgaga aagtaaaggg catcatcacc gtggtaataa
     4861 acgcaggcac cgattcggta aaatcatccc agttaacgcg cgccaggctc gaagtcatca
     4921 gcacgccaac aaagattaac gctccagcgg ttgcgtaagg aggaaccatc gccaccagcg
     4981 gtgagaagaa cataactaac aggaacataa cgccgaccac gacagccgtt agccccgtgc
     5041 gaccaccgac tgccacacca gaagtacttt caatataggc agtaacagac gaggtaccga
     5101 taaatgcgcc tgccaccgaa ctgacgctat caacatacag cgccttattc atattgggga
     5161 atttaccgtt gctatctatt aagcccgctt tatcagttac accaattaat gttcctgatg
     5221 aatcaaatag gttgatcagc ataaaggaga aaatgatacc ggcgagttca agtgttaacg
     5281 cgccgctcaa atctacttca ccaatgacgc cgctaatatc aggcggaatg gaatagacgc
     5341 cgctaaaatg aacatcaccg aaaaataatc cacagcaaga cgtcaccaca atagaaacca
     5401 gcaccgcggc atgaaaatga cgtgatgaca acacggttat aataaaaaac cctaaaatac
     5461 ctaacaacac gccgtgagaa cttaaatcgc caatcatcac cagcgtgtct ttattggcga
     5521 caataacacc agtgtttttt aatcccatta aggcaataaa taatccgatt ccactggtga
     5581 taccaatacg taaacttaat ggaatgttgg agatcatcca gtaccggata cgaaacagcg
     5641 tgagcaaaaa tagcccaatt gcgccccaga atatcgcgcc catcccggtc tgccaggaga
     5701 tccccatcgc ccccacgacc acgaacgcaa agaaggcgtt cagccccatt gccggagcca
     5761 gcaccacggg taagttagca aatatcccca tcgcaatact gccgataccg gcaatcaaac
     5821 aggtggtaac aaacaccact ttcgggtcca tttgtgccgc gccgaggatt tgcgggttca
     5881 caaaaacaat gtacaccatg gttaaaaaag tggtcatccc cgcaatcaat tctgtgcgga
     5941 cggtggtccc atgctgaggt agtttaaata atcgcgaaag cgtccctgat tcattactca
     6001 cgtaatcggt attgtcatta ttcatttttt tgtccattgg aggagattta atcccttgct
     6061 tttgtaaaac aaatgacaac aacggttcag tgataattat cacatttcaa ttgcacatta
     6121 atagatattc tttaataatc tcgcgacgtt tctttatgat aaataataat caaattgata
     6181 aaatcaaaat gagaaaaata tgaataattc tattaaccat aaatttcatc acattagccg
     6241 ggctgaatac caggaattgt tagccgtttc ccgtggcgac gctgttgccg attatattat
     6301 tgataatgtc tctattctcg acctgatcaa tgccggagaa atttccggcc caattgtgat
     6361 taaaggacgt tacattgccg gtgttggcgc agaatacgct gatactccgg ctttgcagcg
     6421 gattgatgct cgcggcgcaa cggcggtgcc agggtttatt gatgctcacc tgcatattga
     6481 atccagcatg atgacgccgg tcacttttga aaccgctacc ctgccgcgcg gcctgacgac
     6541 agttatttgc gaccctcatg aaatcgtcaa cgtgatggga gaagccggat tcgcctggtt
     6601 tgcccgctgt gccgaacagg caaggcaaaa ccagtactta caggtcagct cttgcgtgcc
     6661 cgctctggaa ggctgcgatg ttaacggtgc cagttttacc cttgaacaga tgctcgcctg
     6721 gcgggaccat ccgcaggtta ccggccttgc agaaatgatg gactaccctg ccgtaattaa
     6781 cgggcagaat gcgctgctcg ataaactcga tgcatttcgc tacctgacgc tggacggtca
     6841 ctgtccgggt ttgggtggca aagaacttaa cgcctatatt gctgcgggta ttgaaaactg
     6901 ccacgaaagt tatcagctgg aagaaggacg ccggaaatta caactcggca tgtcgttgat
     6961 gatccgcgaa gggtccgctg cccgcaatct caacgcactg gcaccgttga tcaacgaatt
     7021 taacagcccg caatgcatgc tctgtaccga tgaccgtaac ccgtgggaga tcgcccatga
     7081 agggcatatc gatgccttaa ttcgccgcct gatcgaacaa cacaatgtgc cgctgcatgt
     7141 ggcatatcgc gtcgccagct ggtcgacggc gcgccacttt ggtctgaatc acctcggctt
     7201 actggcaccc ggcaagcagg ccgatatcgt cctgttgagc gatgcgcgta aggtcacggt
     7261 gcagcaggta ctggtgaaag gcgaaccgat tgatgcgcaa accttacagg cggaagagtc
     7321 ggcgagactg gcgctgtctg ccccgccata tggcaatacc attgcccgcc agccagtttc
     7381 cgccagcgac tttgccctgc aattcacgcc cggaaaacgc tatcgggtca ttgacgtcat
     7441 ccataacgaa ttgattacgc actcccgctc cagcgtctac agcgaaaatg gttttgatcg
     7501 cgatgatgtg tgctttattg ccgtacttga gcgttacggg caacggctgg ctccggcctg
     7561 tggtttgctc ggcggctttg gcctgaatga aggcgcgctg gctgcgacag tcagccatga
     7621 cagccataat attgtggtga tcggtcgcag tgccgaagag atggcgctgg cggtcaatca
     7681 ggtgattcag gatggcggcg ggctgtgcgt ggtacgtaac ggccaagtac aaagtcatct
     7741 gccgttaccc attgccgggc tgatgagcac cgacacggcg cagtcgctgg cggaacaaat
     7801 tgacgccttg aaagccgccg cccgtgaatg cggtccgtta cccgatgagc cgtttattca
     7861 gatggcgttt ctttctctgc cagtgatccc cgcgctaaaa ctaaccagtc aggggctatt
     7921 tgatggcgag aagtttgcct tcactacgct ggaagtcacg gaataataaa aaaagcccgg
     7981 cgtcatgccg ggcaaaagtc accagttacg tttatgccac tgtcaactgc tgaatttttt
     8041 tctcgcggcg gattttgcgt tcttccatta ccgccactat cgccatcagg cagatacaac
     8101 caatcgcggc gatatccagc gcggcgaagg tgcctgccca gccggtaagg ccgaataccg
     8161 gcgtcccatc ggcaatcatt cccagaccta acttggcaaa gctgtcacca atcaggtaag
     8221 caaaggtgcc tttaatacca tcggcagcgc caatcgcttt tttaggtaca aagccaacag
     8281 cagccacacc aatcaacaat tgcgggccaa agaccaggaa acccaacgca aagagagaag
     8341 ccagatagat atattcgtta ctggcatgtt gatacacacc gagcgtggcg ataatcagcg
     8401 ccagcgcgat gcaggccacc aggccacggc gaccgttcgc caggtcagag agccagcccc
     8461 acagcagcgt accgaccagc gcaccagctt caaacagcgt aaagccctga atcgccaccg
     8521 ctttagagag tttcagttcc tggaacgcgt atacggttga ccactggtcg ataccaatac
     8581 gtaccacata gaggaaaatg ttggcgaagc acagcagcca gatcactttg tttttcagca
     8641 catactcaac aaagatctgc cacttggtca tatcggtaga ttctgtctct ttgtcctctt
     8701 cgctgacctc ctcgccgaac agttcttcag ctttgccgag gccataagat tccggggagt
     8761 cgctaccgta acgcaggccg ataaaaccga caatcagcgc gataatcgac gggaagataa
     8821 acatgccgat gacatggcca tcgaacaggt aatttgcccc gaacagcgcc acacctgctg
     8881 cgcctgcacc gccaaggttg tgagaaatat tccagaaacc gaggaatgtc ccgcgtttac
     8941 gacgcggcgt ccatttggtg atggtggagt aactgcacga accgccggta ctctggaaaa
     9001 agccgcttaa ggcgtagaag gcaatcatca ggaacaggct aaccgagccg ctgcccatac
     9061 tggcactgaa gcccagcata caaatggcag agaggataag catgaacggc aggaattgtt
     9121 tggtgttttt gccgtcggcg tagtatgaaa ccaacgtttt acccacgcca taagtgatgg
     9181 agaaacccag gccgatcatc cccagctgcg tcatgctcaa cccgtaggtc gaaatcatat
     9241 cgttctgcgc gatgttaaag ttcttgcgaa tcaggtacat cgtcaggtag ccgataaaga
     9301 ccaccaggta ggattgcatg aacggtttga accacatttt gcgccgcact tcgagcggaa
     9361 ggtccagggt cggcttgcga acctggttta ggaaagccag catgggttac tcctgaaatg
     9421 aatacctgcg tgaggcatgc attgtaaaaa tcagcagcaa atcaggcctg agatagcgtc
     9481 caagaaaaac ctggaaaatt tcttagtttt gccggatatg aagtgaaaag gtgagatgca
     9541 tcacgcttca cgcggtgtct gggcgttcaa aaagggcagt aacagcagtg cggaaatccc
     9601 ggcggcgata gcgataacca caaaaaatcc gctccagtgc caggtatcga gcactttcgc
     9661 cagcggccaa ccagcaagcg acgcccccag ataagcaaac aagccgacaa accccgtcgc
     9721 cgcccctgcc gcctctttgt gggaacactc tgccgccgcc ataccaatta acatctgcgg
     9781 gccaaagaca aaaaaaccaa tggtgaagaa gcaggttgcc tgcatcacgt agctggcaaa
     9841 tggcatcagc cacagcgagc cgactgaaag caaaattccg gcggcgaaaa tcaaattcat
     9901 cggcccacgg ttgccgttaa acaatttgtc cgagccccaa ccggctacca gcgcaccgat
     9961 aaatccgccc agttcaaaca tcgtcactgc cgtattcgcc gtgaccagat cgacgcccag
    10021 cgtctcggac atatacaaat tgccccagtc gttgatcgcc gcccggacca catagaccag
    10081 cacatagcaa aacgaaagca gccagatata cggattcagc aacacatatt tggtgaggat
    10141 ctctttacgc gtcaaccctg ccccttcttg ttgttgagca atttccagcg cgtcgtgccg
    10201 ccattcaccg accgccggta aacctaacgc ctgcgggcga tcgcgtagcc gccagcagag
    10261 aaaaatcccc acgactatcg ccatacaacc agcaatcatc atcccggcac gccagccgta
    10321 atgcagcgcg ctcgctgcca tcacaatggg aatgagtgcg ccgccgacgt tatgcgccgt
    10381 gttccataat gcccaccaac cgccgcgctc ggtacgtgaa taccaggccg ttaacagacg
    10441 cgcacacacc ggtgaacccc agccctggaa aaaggcgttc agcacccaga gcacggcaaa
    10501 cgcccatagc gacgtcgaga agccaaacag aatgttcatg atgcccgtgg cgataagccc
    10561 tatccccata aaataacggg catttgagcg atcgctgaca atgccggaga caaacttcga
    10621 cacgccgtaa gtaatgtaaa acagggtcgc taacaggccg atatcgctac ggctgagcac
    10681 gccgttagca aggatttctg gtaccgcggc gttaaaactt ttccgcgtga agtaaaacag
    10741 cgcgtaaccc agccagatgg tcagcagaat atgccgacgc cagtagcgat aacgggcatc
    10801 aatttcatgt ttatcagtca ttaatggcgc atcggcaggc gctttcagaa acggcaacat
    10861 catcgaacct tagacataac gttgaggtag agaaacgctg acacgcgtgc cgtgcagaca
    10921 ggaaatgtgt aatgtgccgc ccagcgccgt tacgcgctcg cgcattccgg tgaggccaaa
    10981 accttgttgc ccggaacccg gcggcaaacc gctgccatcg tcttcaatca ccagcatcaa
    11041 ccgttcatcc tgctgccagc cttgcagcgt aaccgcgctg gcatcggcat gtttcacaat
    11101 gttgttcagc ccttcctggc agacacgaaa cagcgtcacg cgctggtttt cgcttaatgc
    11161 tgattcatcg atacgccatt cgagatggct gacaatgccg cgcccttcca gctccatttc
    11221 ccgcatcagt gagcggatgg cctgctccag ggtgagatca tccagctggc gcggacgtaa
    11281 ccgtcctaac aaacggcgca ccgcgtcgta aacgcccagc gacagttgtt cgatgagctg
    11341 cccgctctgc ttcacactgg cgttatctgc cgccagccgc tgaacaatgc ccgcctgagt
    11401 acgaatagca gtgatggtct gaccgatatc gtcgtgcagc tcacgcgcca cgtcgcggcg
    11461 cacgctctct tcggtttcta acaaacgttc agccagatgc tgattgcgcg ccagttcctt
    11521 ttgtagcgac tggttaagtt cacgcaaccg ctggatgcct gcgcccagta acaaccctgt
    11581 cagactttgc accagcaacg agagtaataa atccaccaga tgatcgcgcc aggtttgact
    11641 ggcgatcagc gcgatggcgt tcatcaacgt cgcaatcagc gctccttgcc agccatagtg
    11701 ccaggccagc gcgataatcg gcagcgccag acagaatggc gtaaagcgcg acagttcgtc
    11761 cggcaatccc aactggagcc agagactgat aacaaacagc agcaagtacc agaccagatg
    11821 ccgcccgcgc cagttgatgg gctgagaaac cagtgacggg ccgagcggca gccaggtgtt
    11881 attggcgaga tagtgccaga acaccagaca tatcggggcc agcgtcagac cgccagttaa
    11941 agtcagcaac agcgcattcc acgactcttt gccgtgccaa agccagggca gcgactgcaa
    12001 caacgccgcc gccgttaacg ccgcaccctg taacagcaag gtgcgccagt cacgctgatg
    12061 gcgatagcgc gagatcagcg ctacgggcag taacgtcagt aaactaccga tcatcaataa
    12121 cggaaaatgg gttaaaccga ccgcctgcgt tagccagtaa atcagcagcc actccgcacc
    12181 cagcaatacg ggccagtatc cgcgcgggca ttgcagcatt agccccagac gcagaccaaa
    12241 cggaaataac agcaccgcca tatcagggcg ctcaaccaga tgcaggctga tactccatag
    12301 gcaaaaccat gcggcagaga agataaaaaa gcaggcaata acggtaatta agcgggagaa
    12361 caacgtcttc atcaccagcc atcaaacatg cggcgcgcca gctctacgtc gttactgacg
    12421 cccagttttt ccatcagatt ggcgcgatgg acgtgtaccg ttttcggtga caagcccagt
    12481 tcggcggcaa tctctttcac cgccattcct tgcgccagtt tttccgccac ctggcgttca
    12541 cgtttggtca gcgggtcctg acgaccggat gccagtttaa tggcaatatc cggcgtcaga
    12601 taacagccgc ccgtggcaac cgtatgcacc gcagcaatga gttcatccgg gctacagcgt
    12661 ttggaaagaa agccgcgtgc ccccgcgtta agcgcctgct caaccagcgc aggactgtcg
    12721 tgaacggaga gcataatcgt cgccatacct ttcggcagct ggcttagcag ctccagaccg
    12781 gagatatcgg gcatggagat atcgcaaata cacacctgca caccgcgccc cggcagcccc
    12841 gccagcgcct cgcgccccga accaaactcg gcaactacct gcaaatcagg ttccagcccc
    12901 agcagctgcg caaagccgga gcggacgatg aggtgatcgt ctataagggc aacggtgatc
    12961 atggtcttgt cctggcgggt aaaaaaaaac gcgcttacct taacgataag cgcgatgttg
    13021 ttcaagcctt gagcggttac tgaaaaaaca ccgcgatctt gttaaacatc gtcggatcgg
    13081 actgattacg ctgcactttc acgacatctt ccagcttatc gatttggctt atcatctgct
    13141 ccagacgctg gtcgtcattg accagtagcc agatatggct tttgtcgctg tcctgaatcg
    13201 gcagacaaag aatgccttca acgttaaaag cgcggcgggc aaaaaggcca caaacgtggg
    13261 tcattacgcc cggatggttg cgaacggtga gctccagaat tacgttgtta tgagttgtgt
    13321 tttgcatggc ttattccccc accatttcag tattcgccgc acctggcggc accatcgggt
    13381 aaactttttc ttcggcatca atgcgcacat ggatcagcgc cgggccaggg cgattgatta
    13441 tttcctgcaa tgcagcctgc ggatcggctt cgttattcaa atcacaggtt tcgaggccga
    13501 atccggcggc aatctgcata aagttgattt tgcccggata ggtggcggca aaaacgcctt
    13561 gctcgtagaa cagactctgt tgctgatgca ccagccccag cgcttcgttg ttcatcagaa
    13621 tgattttgac atccagctga ttttcactgg cggtcgccat ctcctgaata ttcatcatca
    13681 ggctgccgtc gccggagaaa cacaacactt tgcgatccgg gttcgccagc gccgcgccaa
    13741 tcgccgcagg caggccaaaa cccatcgtgc ccagcccacc ggaggtcagc cactggcgtg
    13801 ggcgattgag cggataagct tgcgcggtcc acatctgatg ctgacccaca tcggtggtga
    13861 taatcgcatt gtcatcgaca caggcggcaa cggcgttgat caggccgtaa tggcttaatg
    13921 gatcgcacgc tttcgggatt ggacacggga attcgcgctg caaatccgct accaactggt
    13981 gccactctgc acgcagttgc gcttccacct gcgggatcaa ttgcgccagc acgtcatcaa
    14041 catccgcctg aatcgccaca tgcggctgct tgattttacc cagctctgcg cgatcgatat
    14101 cgacatgaat aattttggca ttcggacaga actgctcggt tttgccaatc gcccggtcat
    14161 caaaacgcgc accgagcaca atcaacaaat ccgcctcttg caaaatatag ttggtactgc
    14221 gcacgccgtg catccccagc atacccagcg acaacggatg cgcttttggc agcatgccca
    14281 gcgccattaa agtcatggtg gtaggcagtt gcgctttctc cgccagttca cgcacccgcg
    14341 caggcgcatt gatcacacca ccacccagat aaagcaccgg gcgtttggca gcgttaatca
    14401 ttgtagctgc gtcacgaatg ctttcttcgc taaaggaggg tgcagcggct ttttctgcca
    14461 ccgcgggctg agcttcaatc tcaaaaaccg ccgtttgtac atccttagga atgtctatcc
    14521 acaccgggct agggcggcct gattgcgcaa tgcggaaggc atcgctcatg acctgtggga
    14581 gctcttcgat atgtctgacc agatagttgt gtttggtgat ggggatagag atgccgtagg
    14641 tgtccacttc ctggaaggcg tcggtgccga tcatcgaggc gggaacctga ccagtgatgc
    14701 aaatcagcgg gatggagtcc agccgcgcat cagcaatggc ggtcaccagg ttagtcgcac
    14761 ccggcccgct acaggccata cagaccgccg gtttaccgtc ggtgcgcgcc attccctgag
    14821 cgataaaccc cgcgccctgt tcatggcgag ccagaatatg gcggatttgc gtgctttggc
    14881 ttaaggcgtc gtaaacaggc aggatagaac cgcccggaat gcccgtcaca atcttaatgc
    14941 cctgctgttc caggaaatga acgataaatt ctgcgccggt aaagcgctta cgcgtcgatg
    15001 ttgtgcccga acttgccatg ctccagtcct tttcttctgg gccgactttc cgggaggtgc
    15061 ttaaacgaaa aaccccgccc ggtttgcgcc ggcggggttt tggaatcgtg tgttgttcca
    15121 gtccctacgg cgcattgccg acgaccacca ccacacgcac gacgaccact gcggcggatg
    15181 gcgcagttgg tagtagtttt gcgttgaaca tggaagtagt cattggggac cttgttggtt
    15241 ttgtgtttaa caatatttat acaagcacag ctttacaggg gagacaatgg aaaatttttc
    15301 agcaagggaa aattgagggg ttgatcacgt tttgtactga attgcagata acaaaaaacc
    15361 ccgccggagc gaggtttcgt cagccgcctg cggctggtaa ccgcaaagca cactgtatta
    15421 tgtcaacact gaaagtatac gtgttccgcg cagaacgcgc aatttcggca cgaattttga
    15481 cgtatttagt gcatagttga gtatcgttca cagtttgcgt tttgtccaaa tattactgtt
    15541 tatttataca gtaaacttct ataatatcac tgttcgcaat gtgttatgcg ggggccgcat
    15601 cgttacccgg cgcactaagt cctggctgaa acgggtggtg ccgtcagcgc cttaaccccg
    15661 cgtgagcaca ctgtgttatg tcaacaagca caacgtttct ccttgagata ccgcgtgcac
    15721 aacagctggc aacaggcagc ggaaaggtac gtcagctggc agtgctcctg aaccacagga
    15781 gacgcgtatg agcctggtgg atatcgccat tcttatcctc aaactcattg ttgcagcact
    15841 gcaactgctt gatgctgttc tgaaatacct gaagtaattc agattcaagt cgcaccaaag
    15901 gggagcgggt aaccgctccc cttttatatt tagcgtgcgg gttggagtcg gatgcgatgc
    15961 tgacgcatct tatccgccct accatccctc ccggcaacat ttattgccgc ttttgtttac
    16021 atattctgcc gctaaacaat tccccattcc tggcgtatat ctggctaaca ttcatcaatg
    16081 tgatagattc ctctcccgca tttatgggaa tgcgtagtga cttattctaa ttatttttat
    16141 aaaagcatcc gtgataatga aaaggcaaag aaacgtcaat ttgttattga tgttggtatt
    16201 actcgtggcc gtcggtcaga tggcgcaaac catttatatt ccagctattg ccgatatggc
    16261 gcgcgatctc aacgtccgtg aaggggcggt gcagagcgta atgggcgctt atctgctgac
    16321 ttacggtgtc tcacagctgt tttatggccc gatatccgac cgtgtcggtc gccgaccagt
    16381 gatcctcgtc ggaatgtcca tttttatgct ggcaacgctg gtcgcggtca cgacctccag
    16441 tttgacggta ttgattgccg ccagcgcgat gcaggggatg ggcaccggcg ttggcggcgt
    16501 aatggcgcgt actttgccgc gtgatttata taaacggaca cagttgcgcc atgctaacag
    16561 cctgttaaac atggggattc tcgtcagtcc gttgctcgca ccgctaatcg gcggtctgct
    16621 ggatacgatg tggaactggc gcgcctgtta tctctttttg ttggttcttt gtgctggtgt
    16681 gaccttcagt atggcccgct ggatgccgga aacgcgtccg gtcgatgcac cgcgcacgcg
    16741 cctgcttacc agttataaaa cgcttttcgg taacagcggt tttaactgtt atttgctgat
    16801 gctgattggc ggtctggccg ggattgctgc ctttgaagcc tgctccggcg tgctgatggg
    16861 cgcggtgtta gggctgagca gtatgacggt cagtattttg tttattctgc cgattccggc
    16921 ggcatttttt ggggcatggt ttgccgggcg tcctaataaa cgcttctcaa cgttgatgtg
    16981 gcagtcggtt atctgctgcc tgctggctgg cttgctgatg tggatccccg actggtttgg
    17041 cgtgatgaat gtctggacgc tgctcgttcc cgccgcgctg ttctttttcg gtgccgggat
    17101 gctgtttccg ctggcgacca gcggcgcgat ggagccgttc cccttcctgg cgggcacggc
    17161 tggtgcgctg gtcggcggtc tgcaaaacat tggttccggc gtgctggcgt cgctctctgc
    17221 gatgttgccg caaaccggtc agggcagcct ggggttgttg atgaccttaa tgggattgtt
    17281 gatcgtgctg tgctggctgc cgctggcgac gcggatgtcg catcaggggc agcccgttta
    17341 agcgcacgtc accgcagcat cgtcatcagc tccatgggag aacgatgctg ctttatcaga
    17401 tcacgcatca cccgcatatg cggtgcggag taagaataaa acgcctgata gcccgcacaa
    17461 agcacgctgt ctggcgtctc ctgccgagcg tgcgggcaca ggcgatgcca gtcgcacgcc
    17521 tggcagtcac ggcgcggttg cgggttttcg ctgccacacc atttttgtaa ggtggcgtca
    17581 aaaagctcaa tcgagatacg actgatatct gcccgcaccc aacactcaaa cagggcaatt
    17641 aaaaatttcc cccacagctc tgctgtcacc gaagcggccg tcagatcgcc ccggtcatca
    17701 aattcaagat ggggaatgag ctgtacggag cggatgcccc gctgtttgaa attgtgataa
    17761 aaatcggcag gttgcagagc atccttgtac tctacaacca gcttatgcct gtcctcttca
    17821 gcatcaatga cctgacttcc tgtcatgcga ctctctcaat aaatacgata agttgatgta
    17881 tatgcgttac agcaaacagt attgcgagag ataacaccgc gagggagatc aaaaatttgt
    17941 cacggactac gtgaaattgg gaaaaatcgc tattcgtgac atccattaaa ttgcgattac
    18001 gggtgtagtg ccagaggatc agcgccacga tggcgaggat gccaatcgaa atccagaata
    18061 acatgcccgc ctggtgccag ttgtgtttga tagccaacgc catcaacgcg ccgtagccca
    18121 gcatggtacg aaaccacgcc agtgatgtgc gctccggctg cagccctggg tcggcaatgc
    18181 gtctggcttt acggctatcc ggcatacaac accagtccca taacgatcac cgccacaacc
    18241 atcaatatta agctgatgat taataagctg ttggtgtagg gcaggtcctc tttcagacgc
    18301 atcgcttttt cattgcgtaa ccagcgcagg tagccataca ttgctaaccc gccggaaaac
    18361 agacacaaca acaacgccag cagttcacga atgacgggcg tggcgaaatc tggcgcaagt
    18421 tgatcaagcc caacccccgc cgccagaaat cctagtgcgg tacggatcca cgtcagaaaa
    18481 gtacgctcat tagccagcga gaagcggtaa tcgggcgctt ctccaaggcg ggaaattttc
    18541 attgttgctc ctttgcaatt tcactaacgc tctatcttta gcggtttttt ttaagaattt
    18601 cgtccgcccg tttcctgata tgcttttgcc atccgctaca aggaggtcag atgggtatca
    18661 tagcgcaaaa taaaatcagt tcattgggca tgctgttcgg tgccatcgct ttaatgatgg
    18721 ggattatcca cttttcattc ggcccttttt ccgcaccacc gccaacgctt gaaagcatcg
    18781 tggcggacaa aacggctgaa attaaaagag gtcttctcgc cggaataaaa ggcgaaaaaa
    18841 ttaccactgt ggagaaaaaa gaggacatgg atatcgataa aatcctcgat caaagcggta
    18901 tcgcactggc cattgctgcg ctactttgcg catttattgg cggtatgcgt aaggaaaatc
    18961 gctggggcat tcgcggcgcg ctggtttttg gtatcggcat cgtgtgcagc attttgctga
    19021 tattcctgat tttttccttc ctgaccggcg gttcgctggt ttaagcagcc tgaataatgc
    19081 ccgccgggtg atcatcagcg ggcattatcg tcagaatttc tgttttttct cctccacctt
    19141 cactccttgc gtcggtaggc cggtgtcata gtcgcgtacc actggcgaat agccatcttg
    19201 tgggcgtggg cgaaacgccc ccatccagcg cggtcgtgca tctttacgcc acggacgcag
    19261 actccattgg taactgcgga acggatcgcg aattttgtcc atgtaatcca ataaggcgtc
    19321 atgcattttg ctgcgaacgt ctgcaaaacg gatatcatcg atcaggttat gcatttcatt
    19381 tgggtcatta cggcgatcgt aaagttcatc actggtgaag aggttgagca ccagtttaaa
    19441 gtcatccgtc acccagcaac gcaccggaat aaaaccgcca aagctgtcat gctcaatctc
    19501 gtagcggtta aattccacca tcacgccgcg tggctctttc acggcaagga tattttcccc
    19561 cggcagaatc tctggttttt caatatctgc cagcgccatc attgtcggca gtaaatcaat
    19621 atgactgact ggcgtatcga cctgtcgccg ctcctttact gatcagctta tgtgcgccca
    19681 tcatttcacc gtgatcggag gtataaatga cccacgtatt ttcacgttgc tctggcgtta
    19741 aggcattgat gacacgtccg atttggtcat caacaaagtc attgcaggca aaatagagcg
    19801 gatggtgata aagcccgtca tcaccgactg gcgatggcat cgcctgcgcc cataagcggt
    19861 gatgttccgg tttgttcgcc aggtcatcct gtgatttctc gccaagttcg tagtaaaaat
    19921 cagcgtattt ctctaaatac tccaccggac aggtgaacgg gtgattcggc tcatcatacg
    19981 aaaccaccat caggaagggt tcctcggcgc gcacgggctg ttgcagaaaa tctaccgctc
    20041 gattgctgat gcggtgcgcc caggtgaagg tttcgtcaat atggttcgcc tgtaaatctt
    20101 cgacgctgtt taggccattg cgccacaggc tgatctcttt ttccgtcagt tcgctaagat
    20161 agttcgcccc atcaaaccag taatcagcat cccactccgg cggacactcg ccagtgccga
    20221 aatagtcatg accgtcgaga tgccatttgc cgatgtaaca ggtgtgatag ccggcatcct
    20281 taaagtagcg ccccatagtg gagatgtttt tgcctggcgc gacgttgttg gtccacgggc
    20341 cggactggtt agcgtagata ccggtgaata gtccggcgcg tgcaggcgta caaaccggtg
    20401 aacaagtgta ggcggaatta aagcgaatac cttccgccgc cagactatca atattttgcg
    20461 tattcagcgg tttaccgcta tagcaaccga ccatattggt ggcctgggta tcggtcaatt
    20521 ggggcgtttc ataggctatc cttcgcgtcg ggagtaaagg ttacggctgg gtcgtgacga
    20581 tgccgccagc tgtcaaaaat caggtagaca atcactacgg cagcgatgaa ataactgatc
    20641 atcgctaacc agcgcgtacc gtagccgccg aattcagcca gcccgacata gacgccaatc
    20701 atcgcgaaca ggataccaat tgacgcgatc ttgacgtttt tccacggttt catgtccacc
    20761 gcaaacgcat ctttgaaggt gaacggcgtg gcgcgcggtt tgataaaacc gatcaccagc
    20821 atcacgacca cgttgatgca gaacgtacag gccagcacat agaggaaatg gaagtcgaac
    20881 ttcaccagat agttgatggt gatgtagctg attatgccaa tccccatcgc cacttttgcc
    20941 gccagcgccg ggatgcgcgg gaagaaaaag cccataatga tgatggtaac cagcggcacg
    21001 ttgtaaatgc cgttgagctg tttcatccag ctatacagcc cctgcggcgc gttggcgatc
    21061 cacggcgcta ccaaaaccga aacgatagcg ataaagaaac caaatttgcg cccgacggtg
    21121 accagctgct gcggctcggc attctggtta atgatgcgac ggtaaatacc catactgaat
    21181 aatgtactgg cgctattcag aaagccgttg aaggtactga tcaccgcacc aaataacacc
    21241 gcgccgaaga aacccaccag tggcactggc agaacgttat tgaccagcgt cgggtaggcc
    21301 atgtcggctt tcggtagatc ctgatacaga tgaaacgcaa tcaaccctgg cagcaccagt
    21361 accagcgggt cgagcatttt cagcaccgcc gttaacagcg cccccttttg cccttccgcc
    21421 aggctttttg acgccagcgt gcgttgcacg atgccctgat ttgtacacca gtaaaaggtg
    21481 ttcaccagaa ttaaaccggt aaatgccgcg ccaatcggca agggatcggt cgggccaccg
    21541 actgagttta atttctcggc gtgaacggtg gtaatttgct caatgccctg cataaagctg
    21601 cccttgccca tcgcgatcag gccaaatacc ggcaccatca acccgccgat aaccagccca
    21661 ataccgttga tggagtccgc cactgccatt gcgcgcagtc cgccgatcac cgcatacaaa
    21721 attcccgcca gaccaagcaa aattaccagt agccagatag ccgcaccgtg agaaatctgt
    21781 agcgattccc cgacgtgaaa caggctgttg agcgccaacg cgccggagta gagaacaatc
    21841 ggcagaaagc agacgccggt ggcaatgagg aagcagaagt cgatgataat acgcgtcgtt
    21901 ttatcataac gttcctccag aaaatcgggg atggtggcaa tgccgcgctt cagatagcgc
    21961 ggtaaaaaga ttagcgcgag gaagatcagc gtcaccgctg aagtcacttc ccagcccatc
    22021 accgacatgc cgcttttgta ggcctgcccg gaaagaccaa ccagttgttc cgtggaaagg
    22081 ttggttaaca ttaacgaagc ggcaataacc ggcgctttta gtgaacgtcc ggcaagaaaa
    22141 tagccttgtt gcgatccggt atctgttttg cgaaccttcc actaggtgat caccgccacc
    22201 agcagcgtaa aaccgacaaa actcaagatt tgtaacgaat tcatctcata gcccttattt
    22261 atgtttatgt cccggacgca tgaatatagg gagaggtaaa atgtgtcggg tcaggcagtc
    22321 agattcttaa aatacaaacg tcgtatccct gaacggattg ttttctgtta agttcaggtt
    22381 gtgagcataa ttcgccggag ggatattaaa atgaatggaa aattgcaaag ttcagatgta
    22441 aaaaacgaaa ctccgtacaa tattccctta ttgattaacg aaaatgtgat ctccagcgga
    22501 atttctctga tctcgctctg gcatacctac gccgacgagc attaccgggt gatctggccg
    22561 cgggacaaga agaaaccgct tatcgccaac tcatgggttg cggtgtatac cgtacaagga
    22621 tgtgggaaaa ttcttttaaa gaatggcgaa caaataactc tgcatggcaa ctgtattata
    22681 tttttaaagc caatggatat tcactcttat cactgtgaag gtttagtctt ggaacagtac
    22741 tggatggaat ttacccccac cagtatgatg gatattcccg ttggtcagca aagcgttatt
    22801 tataatggcg aaatttataa tcaggaactc accgaagttg ctgagttaat aacttcacca
    22861 gaagcaataa aaaataatct ggcagtcgct tttctgacga aaattattta tcagtggatt
    22921 tgtcttatgt ctgcagatgg taaaaaagat ccacaacggc ggcaaattga aaaattaatt
    22981 gccactttac atgccagtct gcaacaacgc tggagcgtag ctgatatggc tgccacgatc
    23041 ccctgtagcg aagcctggtt gcgtcgtctg tttttacgct ataccggcaa gacgccgaaa
    23101 gaatattacc tcgatgcgcg tctggatctg gcgctatcgc tattaaagca acaaggaaac
    23161 tcggttggcg aagtcgctga tacgctcaac ttcttcgact cctttcattt cagcaaagcc
    23221 tttaaacata aatttggtta tgcgccgtca gccgtgctaa agaatacgga ccagcaccca
    23281 acggatgcca gtccacacaa ttaacgtaac tctggccagt acgctttgtt ggcctccagc
    23341 aactcatcca gaatagcttt ggcgaccgag gcgctcggta ccgttttcga caacgtaatc
    23401 gcctgccaca ggtgctgata tgaacgctgt tcccaggcgt ccacgaccag tttttccacc
    23461 gccacttgct gactcattaa ccctttctga aactgcggga tatcgccgac caccagtggt
    23521 tcaggccgta gtagcgcacg cgcatctgtt tgcgatcttg cagcccaaca atttgcgcca
    23581 tccggctttc aataccgatt ggcatgtcac agatgttgag gattttcgca ttcgggcgca
    23641 gacgacgcgt ggcttctgcg acaattgctg ccgggttgga gtagttgagc atctaggcgt
    23701 ttggtgaata tttttccata taatccacca gttccaggac gccgccaatg gaacgcatgc
    23761 cgtacgctat tccgccgggt ccgcaagttt cctgaccaac aacaccgtgg cgccgtggga
    23821 ttttttcatc cagttcgcgc atcgggtact tgccgacgcg gatgtgcgcc ataacaaaat
    23881 caacgtcgct gaacgccact tcaggatcgg tggtgtaact aaacgcaatg tccggcgctt
    23941 tttctttaag gatgacttta caggcttcgg caatcacttc ctgccgcgcg ccatcgttat
    24001 cataaaattt cagtgcacga agcgggaaac ggtcctgatt cgccaggagc atcaacacga
    24061 tgcctggcgt aaaggtgctt ccaccgcctg cgacaaccac tgagaatttg gtcattatac
    24121 tgcctccgta atggcaacat tttctgctga ttgatgagaa ttaattaagc tatcgagctg
    24181 ttcacgcaat tgggatacat gcagaccaat gatcacctga atggcatcgc cactacggaa
    24241 gacgccgtgc gctcccagct ttttaaagac ttcgtcatcc agcgtttgtg acatgtcatg
    24301 cagtgcaata cgtagacgcg tcgcgcaatt gttaatgctg gagatattgc cgaccccgcc
    24361 cagggcttgc aggataccgg cagcctgatc cagctctttt t
//
LOCUS       DADMGC010000055        24188 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.055, whole genome shotgun sequence.
ACCESSION   DADMGC010000055 DADMGC010000000
VERSION     DADMGC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.055"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(22..594)
                     /locus_tag="JNZ73_002138"
     CDS             complement(22..594)
                     /locus_tag="JNZ73_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732419.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="JNZ73_002139"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="JNZ73_002139"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HAY8732420.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="JNZ73_002140"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="JNZ73_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HAY8732421.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="JNZ73_002141"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="JNZ73_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAY8732422.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="JNZ73_002142"
     CDS             complement(3556..5337)
                     /locus_tag="JNZ73_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY8732423.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYNTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="JNZ73_002143"
     CDS             complement(5330..7102)
                     /locus_tag="JNZ73_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY8732424.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSELVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7590)
                     /locus_tag="JNZ73_002144"
                     /pseudo
     CDS             complement(7132..7590)
                     /locus_tag="JNZ73_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="JNZ73_002145"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="JNZ73_002145"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HAY8732425.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8662..10361
                     /locus_tag="JNZ73_002146"
                     /pseudo
     CDS             8662..10361
                     /locus_tag="JNZ73_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10426..11121
                     /gene="queC"
                     /locus_tag="JNZ73_002147"
     CDS             10426..11121
                     /gene="queC"
                     /locus_tag="JNZ73_002147"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HAY8732426.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11173..11571)
                     /locus_tag="JNZ73_002148"
     CDS             complement(11173..11571)
                     /locus_tag="JNZ73_002148"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HAY8732427.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11665..12036)
                     /locus_tag="JNZ73_002149"
     CDS             complement(11665..12036)
                     /locus_tag="JNZ73_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732428.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNLLVERNLAVLTL"
     gene            complement(12187..14058)
                     /gene="ppiD"
                     /locus_tag="JNZ73_002150"
     CDS             complement(12187..14058)
                     /gene="ppiD"
                     /locus_tag="JNZ73_002150"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY8732429.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNETESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14250..14522)
                     /gene="hupB"
                     /locus_tag="JNZ73_002151"
     CDS             complement(14250..14522)
                     /gene="hupB"
                     /locus_tag="JNZ73_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HAY8732430.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14731..17085)
                     /gene="lon"
                     /locus_tag="JNZ73_002152"
     CDS             complement(14731..17085)
                     /gene="lon"
                     /locus_tag="JNZ73_002152"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAY8732431.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17273..18547)
                     /gene="clpX"
                     /locus_tag="JNZ73_002153"
     CDS             complement(17273..18547)
                     /gene="clpX"
                     /locus_tag="JNZ73_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HAY8732432.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18673..19296)
                     /gene="clpP"
                     /locus_tag="JNZ73_002154"
     CDS             complement(18673..19296)
                     /gene="clpP"
                     /locus_tag="JNZ73_002154"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAY8732433.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19543..20841)
                     /gene="tig"
                     /locus_tag="JNZ73_002155"
     CDS             complement(19543..20841)
                     /gene="tig"
                     /locus_tag="JNZ73_002155"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAY8732434.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21185..21502)
                     /gene="bolA"
                     /locus_tag="JNZ73_002156"
     CDS             complement(21185..21502)
                     /gene="bolA"
                     /locus_tag="JNZ73_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HAY8732435.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21807..22385
                     /locus_tag="JNZ73_002157"
     CDS             21807..22385
                     /locus_tag="JNZ73_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8732436.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22429..23904
                     /gene="ampG"
                     /locus_tag="JNZ73_002158"
     CDS             22429..23904
                     /gene="ampG"
                     /locus_tag="JNZ73_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HAY8732437.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5667 a         6661 c         5837 g         6023 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacggtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattg cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccaga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccgctgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcccacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataattgtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctca gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacgttgg cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcaa gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gttgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gagatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact acgcctactg ccgcccgatt aaacagcagc gactgagcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccagccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgagttcgct accatcgttc accaccggcg cttccgccag catcgcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggatcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctagacgcgc atcagataat cgtattcacc agccatgcgc cagaacccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtcagcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctgc cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 ataatattgt tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg
     8701 tggcaacctt cagccggaaa gccgcaaacc gtcaccgtca gcgaactggc cgaacgctgt
     8761 ttttgcagcg aacgccatgt tcgtacgctg ttgcgtcagg cacaggaggc gggatggctg
     8821 gagtggcagg cgcagtcagg acgcggaaag cgcggacaat tacgctttct ggtcacgccg
     8881 gaatcgctac gcaatgcgat gatggaacag gcactggaaa ccggaaagca gcaagatgtg
     8941 ctggagctgg cgcaactggc cccaggtgag ctgcgcactc tgttacagcc gtttatgggc
     9001 ggacaatggc aaacgataca cccacgttgc gtattcccta ctatcgcccg ctcgaaccgc
     9061 tacaaccagg ctttttgccc ggccgtgccg agcagcatct cgccgggcag atattttccg
     9121 gcctgacccg cttcgataat aatactcagc gcccgattgg cgatttagcg catcactggg
     9181 aaacctctac tgacgggtta cgctgggact tttatcttcg ttcaacccta cactggcata
     9241 acggcgatgc agtaaaagcc tcacacttac accagcgatt attgatgctg ttacaactgc
     9301 cagcactgga tcaattattt attagcgtga agcgtattga agtcacccat ccgcagtgtc
     9361 tgaccttctt tttacatcgc cccgattact ggcttgcgca ccggctggcg agctattgca
     9421 gccatctggc gcatccgcaa ttcccactga tcggcactgg tccttttcgc ttaacacaat
     9481 tcacagcgga gctggtgcgc ctggagagcc atgattatta ccatttgcgt catccgctgc
     9541 ttaaagcggt tgagtactgg ataactccgc cgcttttcga aaaagatttg ggaaccagtt
     9601 gtcggcatcc cgtgcaaatc accatcggca aaccggagga gctgcaacgg gtcagccagg
     9661 tcagtagtgg catcagttta ggtttttgct atttaacgtt gcgcaaaagt ccccgactct
     9721 ccctctggca ggcgcgaaaa gtgatctcca ttattcatca atccggttta ttacaaacgt
     9781 tagaagtcgg agaaaacctg atcaccgcca gtcatgcatt actgccaggc tggactattc
     9841 cgcactggca agtaccggat gaagtcaaac taccgaaaac cttgacgctg gtttatcacc
     9901 taccgataga acttcatacc atggcagaac gcctacaggc gacactggca gcggaaggct
     9961 gtgaactcac aattattttt cataacgcaa aaaactggga cgacacgacc ctactggcac
    10021 acgcagacct catgatgggc gacagattaa ttggcgaagc accggaatat actctggagc
    10081 aatggctacg ttgcgatcca ctatggccac atgttttcga cgctccagca tatgcacatc
    10141 tgcaatcgac gctggacgcg gtgcaagtaa tgcctgatga ggaaaaccga tttaatgccc
    10201 tgaaagcggt ttttagccag ttaatggcag acgcgacgct gacgccgctg ttcaactatc
    10261 actatcgcat tagtgcccct cccggcgtga acggtgtgcg actgacaccg cgcggctggt
    10321 ttgaatttac cgaagcctgg cttcccgcgc cgtcgcaatg aatagctggt ccgggttaat
    10381 cgcaggcgtt accataacgc ttttattgtt caagcaggat tatctatgaa acgtgctgtc
    10441 gttgtgttca gtggaggcca ggattccacc acctgtctgg tgcaggcatt acaacaatat
    10501 gatgaagtcc attgcgtgac gttcgattac ggtcagcgac atcgcgcaga aatcgacgtg
    10561 gcacgcgaac tggcgctgaa actgggagca cgcgcgcata aggttcttga tgtaacgctg
    10621 ctcaacgagc tggcggtcag cagcctgaca cgtgacagca ttccggtgcc tgattatgaa
    10681 cctgaagccg atggcatccc gaatacgttt gtcccagggc gtaatatttt gttcctgacg
    10741 ctggcggcaa tatatgcgta tcaggtaaaa gcagaagccg taattaccgg cgtctgtgaa
    10801 acggatttct ccggctaccc ggattgccgc gatgagtttg tgaaagcact aaaccatgcc
    10861 gtcagtttgg gcatggcgaa agatattcgt tttgaaacgc cgctgatgtg gattgataaa
    10921 gcggaaacct gggcgctggc agattattac ggcaaactgg atttagtccg taacgaaacg
    10981 ttgacctgct ataacggcat taaaggcgac ggttgcggtc attgtgcggc atgtaattta
    11041 cgcgccaacg gtttgaatca ttatctggcc gataaaccga cggtgatggc agcgatgaag
    11101 cagaaaaccg ggttgaggta attattccgg gtgtcgccgg atgcggcttg agcatccggc
    11161 accacaaaac gtttacttaa ccatctgctc cagcttttcg cgcaattccc cttccagagc
    11221 taatgctttc tgcgttttaa gatcaataca aacaaacgta ataagcgcat ccgctaccac
    11281 ctgcccttcc ggctccagtg taatgacctg gcttaagatg ccgcttttac cgtttaattg
    11341 ctgcaactga ctggtaatag ttaacaggtc gcttaatacc gctgggcgac gatagttaat
    11401 attgatattg accacgacga aggcgatgtt atgggccgtc atccactgaa aactgtcgct
    11461 attttccaac ccatcccagc gggcttcttc gagaaattca aggtagcggg cgttgttgac
    11521 gtgctggtaa acgtcgagat gatatccacg aactttgatt tgtgtttgca tagcgcaata
    11581 accttacgtt gttgttataa gtacagaggt cataactggt atgacctctt cagtctggca
    11641 aaaaattgcc actatgcaaa ttaattacag ggttaatacc gccagattac gttccaccag
    11701 caaattgccc atccccggca cctgctttag atcctccacc gttttaaacg gaccgtactc
    11761 ttcgcgataa ctgacaatcg cctgcgcttt cttcaggcca acgccattca tcgcgcgggc
    11821 tagctcttcc gcgctggcat tattaatgct gacccgggtg ccttcttcgt cactggcttt
    11881 cgccggtact gctgctttac tttgtgctgc aggagcttcc gctttggttt ctaccgccgt
    11941 cggtttcgcc acagaagctg ccgccagcgc gctatgagac attccggcac aggccaggga
    12001 cagggtaatg agcagtgctt taattccgtg tttcatactg ttttctcctt gtttgttaac
    12061 agtggaatca ccttaacggg agtgaaaaca gtaaacaaat ggcaatgttc agaaatggaa
    12121 aaggccgcga aagcgacctt ttcttgttac agctgcaacg catttttttg cgaggcggct
    12181 tcaggattat tgctgttcca gcgcatcgcc aattttgatt ttcgcctctt tacgcaggtt
    12241 actcatcaga gcttcaaaga cgatttgtgc gttgttctgg gtgataccct gcaccatcgc
    12301 ttttttctga tcttccggca ttgaaccttg tttcacttca tccagcgcca gcagaaccac
    12361 attaccttgc atatcagtcg ccataccgta gctcggtttg tctttcgctg gcagtggcag
    12421 tgcaaacgcc gcctggctaa tcgggtcacg accggaacgg cttaaggttt tcggctcgcc
    12481 aaatttcaga ccggcagcct gcatagcttc cgcacctttg ccggctttca aatcaaccag
    12541 cagtttctca gcatccactt tcgcctgttg ttcagctttg ttgtgctgaa ccagtgcctt
    12601 aacttgttcc tgaacatctg ccaacggttt caccgcttcc ggtttgtgct cgctgacgcg
    12661 cagcacgaat gcgcggtcgc cgtctacggt gatgatgtca gagttgatgc ccgacgcgcc
    12721 gttttcacct accagaccgc cgttaaagat agcgtcggca accggcttga agttcaactc
    12781 ttccggcagg ttatctttgc tgaaccaacc cgtctgagtg gctttaacgc cggcagcttg
    12841 ctctgcaccg gccagagact cggtttcgtt gcttgccgca tcgctcactt tctgctgcag
    12901 cgcatagtac gcatcgaggg ctttttcgtg tttcactttc gccgcaatgt cgtcacgtac
    12961 ttcgtctaac gatttcactt tcgctggctg aatgtcgtcc agacgtacaa tcaggaaacc
    13021 gaccgaagat ttgatgacac cagacagttg gcctttttct ttcagaccag catttttcag
    13081 ttcgtcgggg atagtggcat cttctaacca acccatatcg ccgccgttac gggcagagat
    13141 aatatcggca gatttttctt tggctaatgc agcaaaatca ccgcctttat tcagctcatc
    13201 aagtaccgct ttcgcttcat cttcagtttt ggtctggatg atgctgtagc gggtacgctg
    13261 cggctgggtg aattgatcct gatgctgatc gtagtagctc tggatatccg catcgctaac
    13321 cggttgctgc atcgttgcgg caaccagctt gatgtaactc acgcggaatt gttccggcgt
    13381 catgaaattg tttttgtttt gttcgtagta gctggcaatc tcctgttcgg tcacaggctg
    13441 cttcgccgcc agcgcgttaa catcgatagt cgcctcacgc accacgcgtt gttgcgcgac
    13501 cagtgccgcc agctcgtcgg tttcaccttt cagcataaaa tcggtaccgg caacgccgtt
    13561 aatcagctgt tgggtagtga gctggttacg cagcgcctgg gcgtactgat cggcggtcat
    13621 ccccatctgg ttgaggatac cgttatagcg gctgttatca aatttgccgt caacctggaa
    13681 ggctggggtc gcgaaaatcg cctgtttaac ctgctcatca ctgataccca gtttcagctc
    13741 acgagcgtac tgatccaaca gcgcctcgtc gatcagacga ttcagcacct gttgacgcag
    13801 ggttttcata tagccttcgt tcgctgccag ctcggagtat tgatcgccca gctgttgctg
    13861 catgcgatta cgctcgctgt taaaggcgtt ctcgaactgc ccacggctga tttcctggtc
    13921 attcactttt gcggcgtagt tattgcctcc gccaatcagg taaccactca cgccggtcaa
    13981 tatgaacgac acgataatga taccgaaaat aatcttgagc acgagactgt ttgcagccgt
    14041 gcgtaagctg tccatcatgg tgtaacaaca ctccgctgta gatgactgtg tacctcacgc
    14101 aacgtatcga tgcgcgcaaa ggcctattgt gacaagaaac gggggcaatt gtcagcccac
    14161 aagacacaga aagtcaccga atacaaataa aaaaggcaca tcagtagatg cgcccttgaa
    14221 cttcgtcaca tccccactgg ggacaacgct tagtttaccg cgtctttcag tgctttacct
    14281 gcacggaagc tcggtacttt agcggcagcg atggtgatct ctttaccggt ctgcgggttg
    14341 cggccagtac gggcagcacg ctctttaacg gcaaaagtac caaaacctac cagtgctaca
    14401 tcatcccctt ctttcagaga ttcagttacg gaagcaataa tagcatctaa cgcacggcca
    14461 gccgcagctt tagagatatc agcccctgca gcaatcttgt cgatcaattg agatttattc
    14521 actcttctct tcctctttat aatttatatc gcacttgaat ccttcaaggt acgaacgcgc
    14581 agcagttata tcaggccagc catcccctta caacacccct taataagggc aagcccgatc
    14641 cgctatctaa cttagcgaga caaaaaaagg ctggcaagcc cgaattagcc tgccagccct
    14701 gtttttatta gtgcattttg cgcgaggtca ctattttgca gtcacaacct gcatgccaga
    14761 cggttcattt tgcagcgcca gagtcagaac ttcctcaatg cgcttcacag gatgaatgtc
    14821 cagatcggca attacgttgt caggaatctc ttccagatcg cgtttatttt cgaacggaat
    14881 tagcactgtt ttaatcccgc cgcgatgcgc tgccaggagt ttttctttca aaccaccgat
    14941 cggcagtacc tgaccacgca gagtgatctc accggtcatt gccacatcgg cacgaaccgg
    15001 gttaccggtc aggcaagaaa ccagcgcggt gcacatagca ataccggcac tcggaccatc
    15061 tttcggcgtc gcaccttccg gtacgtggac gtggatgtca cgtttttcgt aaaaatcagg
    15121 gttgatcccc agtttttccg cacgcgcacg aaccaccgtt aacgccgcct gaatagactc
    15181 ctgcatcact tcgccgagcg aaccggtata ggtcagtttg cctttacccg gaacacatgc
    15241 ggtttcaatg gtcagcaagt caccgcccac ttccgtccac gccagaccgg ttacctgacc
    15301 gacacggttt tcgttatcag cgcgaccata gtcgaaacgc tgaacaccga ggtagtcatg
    15361 caggttatcg ccgttaattt cgatatgttt taatgactta tcgagcagta actgcttaac
    15421 cgctttgcgg cacagtttgg agatttcacg ctccagacca cgcacgcccg cctcacgggt
    15481 gtagtaacga ataatgccga taatggcgct atcgtcgacg gtcagctcac cttttttcag
    15541 tgcattacgt tcaatctgct tcggcagcag gtgacgtttg gcgatgttca gtttttcatc
    15601 ttcggtataa ccggagaggc gaatcacttc catacgatcc agcagcggtg ccggaatgtt
    15661 catggagttc gacgtcgcga caaacatcac gtcgctgaga tcgtaatcca cttccaggta
    15721 gtggtcgctg aacgctacgt tctgctctgg atccagcact tcaagcagtg cagaggccgg
    15781 atcgcctcgc atgtcagaag acattttgtc gatctcatcg agcaggaaca gcgggttttt
    15841 cacgcccact ttcgccattt tctggatcag tttacccggc atagaaccga tgtaagtacg
    15901 gcggtgacca cggatttccg cttcatcacg cacgccgccc agcgccatac ggacatattt
    15961 acgcccggtg gctttggcaa tggactgacc aagagaggtt ttacctaccc ccggcggccc
    16021 taccaggcag aggatcggtc ccttgatttt gttgacacgg ctttgaaccg caaggtactc
    16081 aaggatgcga tctttcacgc gctccagacc ataatggtcg gtatcaagga tttcctgcgc
    16141 ctgacgcagg tcttttttga ccttgctgcg cgcattccac ggtacctgta ccatccagtc
    16201 gatataacca cgcactacgg tcgcttctgc cgacatcgga gacatcattt tcagcttctg
    16261 caactctgct tccgctttct cttttgcctc tttcggcatc ttcgccgcgt cgattttgcg
    16321 cttcagggct tcgttttcgt ccggcgcgtc gtccatttca ccgagttctt tctgaatagc
    16381 tttcatttgc tcgttcagat agtactcacg ctgggatttc tccatctgct ttttaacgcg
    16441 gttgcgaatg cgtttctcaa cctgcagcag atcgatttcc gattccatca ttgccatcag
    16501 atattccaga cgttcgttaa cgtcggacat ctccagaacg gactgtttgt cagccagttt
    16561 cagcggcata tgtgcagcaa tggtatccgc cagacgcgcc ggatcgtcga tgctattcag
    16621 cgacgtcagc acttctggtg ggattttttt gttcagcttg atgtagcctt cgaactggct
    16681 gattgcagta cgcaccagca cttcctgttc ccgctcatca atggtcggcg actccagata
    16741 ctccgccttc gcagaaaagt gttcgccatt gtcagagagc gcagaaatac gcgcgcgctg
    16801 taacccctcg accagcactt tgacggtgcc gtcaggcagt ttcagcatct gcaatataga
    16861 ggccacggtc ccgacggtga aaagatcgtt tacacccggc tcatccgttg aagcttcttt
    16921 ctgcgcgacc agcataattt ttttatcatg gtccatcgcc gcttccagac aacggataga
    16981 tttttcccgc ccgacaaata aggggatgac catgtgcgga taaaccacca catcgcgcag
    17041 cggcaatacg gggatttcaa tgcgttcaga acgctcagga ttcatagagc tctctcttag
    17101 tttaatttcc gccaggtaat cagatgacac gactgtgctt cacgccatct attaacatgt
    17161 acgtcagtat atggggatgt ttcccccaca ttcaacgccg agaatagagg aaaaattaaa
    17221 ggggagataa aatcccccct ttttgtttaa ctaattgtat gggaatggtt aattattcac
    17281 cagatgcctg ttgcgcttcc ggcttaccat aaatcagcaa cggtttgctt tgaccatcaa
    17341 ttaccgactc gtcaataacc actttttcga cgtcttccat ggacggcaga tcgtacatgg
    17401 tatcgagcag tgcggcttct acgatggaac gcaggccacg ggcaccggtt ttacgcgcca
    17461 tcgctttctt agcgatagca tccagcgcct cgtcacggaa ttccagatcc acgccttcca
    17521 gattaaacag cgcctgatac tgcttggtca gggcgttttt cggctctttg aggatctgaa
    17581 tcagagcttc ttcgctcagt tcattcaacg ttgctacaac cggcagacga ccaatgaact
    17641 cagggataag accaaacttg atcagatctt ccggttcaac ctgcgccagc agctcgcctt
    17701 cgcttgcttt gtcggacttc gcttttaccg tcgcgccaaa accaatgccg gagccggttt
    17761 ctacacggtg ggaaatcact ttatccagac cggcaaacgc accgccacaa ataaacagga
    17821 tcttagaggt atcaacctgc aagaattcct gctgcggatg tttacgccca ccttgcggtg
    17881 gaacagcagc taccgtacct tcgatcagtt tcaacagtgc ctgctgtact ccttcaccgg
    17941 aaacgtctcg ggtaatggac gggttgtctg acttacgaga aatcttgtcg atttcatcga
    18001 tgtagacaat cccgcgctgc gctttctgta cgtcgtaatc gcacttttgc aacagcttct
    18061 gaatgatgtt ttcaacgtct tcgcccacat aaccggcttc ggtcagcgtg gttgcgtcgg
    18121 ccatggtgaa cgggacgtcc agcaggcgcg ccagcgtttc agccagcagc gttttaccgg
    18181 aaccggtcgg accgatcagc agaatgttac ttttgcccaa ctcgacacca ttgctggtat
    18241 cgccgttgcg cagacgtttg taatggttgt ataccgcgac cgccagcact tttttcgcct
    18301 gttcctggcc gataacgtaa tcgtccaggt ggttgcgaat ttcatgcggc gtcggtagcg
    18361 cactgcgttc acgatgcggt gcaacttctt taatctcttc gcgaatgatg tcgttacata
    18421 aatcaacaca ttcgtcgcag atatacacgg atggaccggc aatcagcttg cgcacttcat
    18481 gctggctttt gccgcaaaaa gagcaataca gcaatttgcc tgagccatct ttgcgtttat
    18541 ctgtcatggg tcaaaacctc ttctttgttc tttgtgccgc acacgacgac gcaaatgcca
    18601 ttctcaggcg caagccgctt acagcgttgt gccgccctag ataagtatag cggcacagtt
    18661 gcgcctctgg catcaattac gatgggtcag aatcgaatcg accagaccgt attccaccgc
    18721 ttcaggggcg gaaaggaagc gatcgcgctc ggtatcacgt tcaatctgtt ctaatgattg
    18781 acccgtatga agcgccataa gttcattcat gcgcccttta actttcagaa tttcacgagc
    18841 atgaatttcg atatcggtcg cctggccctg gtagccgccc aacggttggt gaatcatcac
    18901 gcgcgaattc ggcaggcaaa aacgtttacc ttttgcccct gcggtcaaca agaaagcgcc
    18961 catcgaggcc gcctggccca tacagatggt gctgacatca ggcttgataa actgcatggt
    19021 gtcatagata gacatcccgg cagtgatcac cccgcctggg gagttaatgt acagatagat
    19081 atctttttct gggttttccg cttccaggaa cagcatctgc gccacaatca ggttagccat
    19141 gtggtcttca acctggccag tcagaaaaat gacgcgttcc ttaagtagac gagaatagat
    19201 atcaaaagag cgctcaccgc gtgaggtctg ttcaatgacc atcggcacca gcgccatatg
    19261 gggtgcaaag ttatctcgtt cgccgctgta tgacatttcc gtctcctgga taaaattgaa
    19321 aaaaacctgc tgtaccgatt gtaaccttat ggacggatta tcagctagtc cctgtgttat
    19381 gggtacggca tcaccatatt tcaagcataa caatcttttg ttgttacgct aacaccgaaa
    19441 gaggcttttc gcacggctcc atgcaaaatt catgacaaaa aaaagcccac cacctggcgg
    19501 tgacgggcct ttgtgcgaat ttagcgcgtt atgctgcgta aattacgcct gctggttcat
    19561 cagctcgttg aaagtggttt ctttttcagt cactttcgct ttcgccagta cagcttcaac
    19621 agcctgttct tccagagcaa cattgcgcat gttgtccatc agttctttgt ttttgctgta
    19681 gaactcgata acttctttcg gatcttcgta cgcagaagcc atctcttcga tcaggccttt
    19741 cacgcgctct tcgtcagctt tcagctcgtt ggtgcggata acttcgccca gcagcaggcc
    19801 aacaactacg cggcgtttag cctgttcttc gaacagttcg cgcggcagtt ccagagcttg
    19861 tttttcgttg ccaccgaaac gctgtgcagc ctggcgacgc agaacgtcga tttcgctgtc
    19921 gatcagcgca gccggtacgt cgatgtcgtt agcttttacc agaccttcga tcgcctgaga
    19981 cttaacgcgg ttacggatgg cgctcttcag ctcgcgctcc atgtttttac gcacttcagc
    20041 gcgcagacct tctacggaac catcttcaac gccgaaacgt ttgatgaatt ctgcagtcag
    20101 ttccggcagt tcacgctctt caactttctt caggttgata gcgaatttcg ctgctttacc
    20161 tttcaggttt tctgcgtggt attcttccgg gaaggtcacg tcgatggtga actcttcgcc
    20221 agctttgtgg cctttgatac cgtcttcaaa gcccgggatc atacgaccct ggcccatcgc
    20281 cagtacgaaa tcagacgctt taccgccttc gaactcttcg ccgtctacag aaccggtgaa
    20341 gtcgatggtt acgcggtctt ctgcttcaac agcgccgtct ttttctttcc aggtcgcctg
    20401 ctgtttacgc agagtatcca gcatgccgtc aacgtcagcg tcggtcactt caacgatcgg
    20461 tttttcaact tcgatcgctt ccagaccctg cagttcaact tccggataaa cttcaaactc
    20521 tacagagtaa gtgaagtctt cacccagctt gtattcgccc ggaacataag tcggtgcgcc
    20581 agccggattg attttttctt taatgatggc gtcaatgaag ttacggctca tcaggtcacc
    20641 cagaacgtcc tggcgtacag acgcgccata acgctgagca acgatattca ttggcacttt
    20701 gcctttgcgg aagccgtcaa tacgtacttt tttcgcaacg ttgaccagct cgcttttaac
    20761 agcggtctcg atgctgtcag cagcgatagt aatcgttaca cggcggccaa ggccttgagt
    20821 ggtttcaact gaaacttgca tcttgttacc tcaaaaaatc acagtgctcg gtcaactctt
    20881 accccgcaag cacaggttgt tggcttcgcg gaaccgggat gttcttataa tcaatcacat
    20941 tccctgttac cagaatcatc ccgaaagcat tcaaatagga cgcggcatta tagcggcatc
    21001 acttttatga gtcgagaacg gttattgcgc gctgtcgcgt attttttcac aattcccggg
    21061 caattttcgt ctgaatgctg ggcaaaaagc gcaaaatcca gacaaaacaa aacggcccgc
    21121 aggccgtttt tcataaaatc tttagcaaca tactggaaaa gcgccgacag ttgcaaatgc
    21181 gtttttacgc gatgcttcct gctccacgac agggaggaga ggcaaagacg gtgtcctgca
    21241 acccttccca ctccttaata gtgtaagtat gcagagccag cgcatgaacg gtagtagaga
    21301 gttcctccgc taaagtactg taaatcattc gatgacgatt cagaaaacgt tcacccgtaa
    21361 aacgatcgct gaccagcaca actttaaaat ggctttcaga gccggctggg acattgtgac
    21421 gatagctttc atccactact tcgaggaata cgggttggaa cgccgccctt aatttttctt
    21481 ctatccgctc acgtatcatc atgaaatatc tcctccgaca acgctgagat gtcatccatc
    21541 cctttaaata ctagccgctt ttaccgtttc cattgcagct taacaacaaa tatttaggtt
    21601 tcgtcctggt ttttccgtca agcgtatgaa gtttactgat ttaaagattg aaacacaggc
    21661 ctcgcatcag cgtttctttt accaggggtg attatttcgt caacatgatg aatttacatg
    21721 ccttacccac ttcccctcgc cgttggcaat gttatgatgg cgggaatttt taccaaacac
    21781 gcttaagagt ctctgagagc ccgaacatgt tcaaaaaaat cctcttcccg ttagttgctc
    21841 tgtttatgct tgcaggatgc gcaaaaccgc caacaactat tgaagtttcc ccgacgatta
    21901 cactgccaca gcaggatcca agcctgatgg gcgtcaccgt aagcattaat ggtgccgatc
    21961 agcgtacaga tcaggcgctg gcaaaagtca cccgcgataa tcaaatcgtt accctgaccg
    22021 cctcccgcga tctgcgtttc ctgctgcaag aagtgctgga aaaacagatg accgcgcgtg
    22081 gttacatggt tggtccgaat ggcccggtta atctgcaaat catcgttagc caactgtatg
    22141 ctgacgtgtc ccagggcaac gtgcgctaca acatcgctac caaagcggat atcgccatca
    22201 tcgctaccgc gcagaatggc aacaaaatga ccaaaaacta tcgtgccagc tacaacgttg
    22261 aaggtgcgtt ccaggcctcc aacaaaaata tcgccgatgc ggttaacagt gtgctgaccg
    22321 ataccatcgc tgatatgtct caggacacca gcattcacga attcatcaag cagaacgcgc
    22381 gttaatttct gccctctggc ccggtgcaag ccgggcctgt agacgcccat gtccagtcaa
    22441 tatttacgta tttttcaaca gccgcgttca gccatattgc tgatcctggg ttttgcttcc
    22501 gggctaccgc tcgccctgac atccggcacc ttacaggcct ggatgacggt cgaaaatatc
    22561 gatctcaaaa ccattggttt cttctctctg gtaggccagg cttacgtttt taaattcctc
    22621 tggtcaccgc tgatggaccg ctacacgccc ccattttttg ggcggcggcg cggttggctg
    22681 ctcgccacgc aaatcctgtt attagtcgcc attgcggcga tgggttttct cgaaccaggc
    22741 acccaactcc gctggatggc ggcgctggcg gtggtgatcg ctttttgctc tgcctcccag
    22801 gatattgtct tcgatgcgtg gaaaaccgat gtgctcccgg cagaagaacg tggtgcgggc
    22861 gcggcaatca gcgtgctggg ttaccgttta gggatgctgg tttccggcgg cctagccctg
    22921 tggctggcag ataaatggtt gggctggcag ggcatgtact ggttgatggc ggcgctgttg
    22981 atcccctgta ttatcgcgac gttgcttgca ccagaaccaa ccgacaccat tcctgtgccc
    23041 aaaacgctgg aacaagcggt tgttgcacct ctgcgagatt tctttggtcg caataatgcc
    23101 tggcttattt tgcttcttat cgtgctgtat aagctgggcg acgcattcgc catgagcctg
    23161 acaaccacgt ttttgattcg cggcgtcggg tttgatgcgg gtgaagtagg cgtggtaaac
    23221 aaaacgcttg gcttactggc gaccattgtt ggcgcattgt acggtgggat tttgatgcag
    23281 cgcctgtcgc tgttccgggc gctgctgatt ttcggcattt tacaaggtgc gtctaacgct
    23341 ggttactggc tgctgtcgat tactgataag catctctaca gcatgggcgc agccgtcttt
    23401 ttcgaaaacc tctgtggcgg gatgggcaca tcagcctttg tcgcgctgtt aatgacgctg
    23461 tgtaataagt cattttccgc tactcaattt gccctgctct cagcgctttc cgctgtaggg
    23521 cgagtttatg tcggccccgt ggcgggttgg tttgttgaag cacacggctg gtcgacattc
    23581 tatctattct ccgtcgccgc tgccgtaccg gggcttattt tgctgctggt ttgccgccag
    23641 acgcttgaat atacacgagt aaatgacaac tttatctccc gtaccgaata tccggcaggt
    23701 tatgcctttg ccatgtggac actggcggcg ggcgtcagcc tgttggccgt gtggttactt
    23761 ctgttgacga tggacgcgct ggatttgacg cacttctctt tcctgcctgc tctgctggaa
    23821 gtcggggttt tagtcgccct ttctggcgtc gtgcttggtg gtttgctgga ttatctggcg
    23881 ctacgaaaaa cgcatctgac gtaatctgta aatattattt agctgtagta atcactcgcc
    23941 gtaattatta cggctaaata aacatcagca tcgcttatta atatttgttc ttttgtgcgg
    24001 cttagcgttt ggaaattatt ggcgccattt ataaattcta tttttcttac acgattcagc
    24061 taatgagtct ttattttctc accacccagt tgtcactcta atgataatta tttgttaaat
    24121 aattgtttta tttcgcattg gttataccaa ttgcccgcca agactccttc tgcactcccc
    24181 ttttgtta
//
LOCUS       DADMGC010000056        24112 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.056, whole genome shotgun sequence.
ACCESSION   DADMGC010000056 DADMGC010000000
VERSION     DADMGC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.056"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..1270
                     /gene="glpK"
                     /locus_tag="JNZ73_002159"
     CDS             <1..1270
                     /gene="glpK"
                     /locus_tag="JNZ73_002159"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAY8732438.1"
                     /translation="TNQRETTIVWEKETGKPIYNAIVWQCRRTAEICEHLKRDGLEDY
                     ISSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTVDTWLIWKMTQGRVHVT
                     DYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEVYGQTNIGGKGGTRIPI
                     SGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVKSENGLLTTIACGPTGE
                     VNYALEGAVFMAGTSIQWLRDEMKLINDAYDSEYFATKVQNTNGVYVVPAFTGLSAPY
                     WDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQADSGIRLHALRVDGGAV
                     ANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQNLDELQEKAVIEREFR
                     PGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            1500..2510
                     /gene="glpX"
                     /locus_tag="JNZ73_002160"
     CDS             1500..2510
                     /gene="glpX"
                     /locus_tag="JNZ73_002160"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY8732439.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLNRKDPEMQVH
                     IL"
     gene            2607..3353
                     /gene="fpr"
                     /locus_tag="JNZ73_002161"
     CDS             2607..3353
                     /gene="fpr"
                     /locus_tag="JNZ73_002161"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HAY8732440.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(3358..3786)
                     /gene="uspD"
                     /locus_tag="JNZ73_002162"
     CDS             complement(3358..3786)
                     /gene="uspD"
                     /locus_tag="JNZ73_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HAY8732441.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLENKSDNKLYKLTKNIQCPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(3813..4112)
                     /locus_tag="JNZ73_002163"
     CDS             complement(3813..4112)
                     /locus_tag="JNZ73_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HAY8732442.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(4324..4764)
                     /locus_tag="JNZ73_002164"
                     /pseudo
     CDS             complement(4324..4764)
                     /locus_tag="JNZ73_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            4865..5464
                     /locus_tag="JNZ73_002165"
     CDS             4865..5464
                     /locus_tag="JNZ73_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HAY8732443.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            5572..6339
                     /gene="tpiA"
                     /locus_tag="JNZ73_002166"
     CDS             5572..6339
                     /gene="tpiA"
                     /locus_tag="JNZ73_002166"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAY8732444.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(6394..7149)
                     /gene="cdh"
                     /locus_tag="JNZ73_002167"
     CDS             complement(6394..7149)
                     /gene="cdh"
                     /locus_tag="JNZ73_002167"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HAY8732445.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKSVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMSTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(7256..8245)
                     /locus_tag="JNZ73_002168"
     CDS             complement(7256..8245)
                     /locus_tag="JNZ73_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8732446.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(8565..9527)
                     /gene="pfkA"
                     /locus_tag="JNZ73_002169"
     CDS             complement(8565..9527)
                     /gene="pfkA"
                     /locus_tag="JNZ73_002169"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAY8732447.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(9708..10610)
                     /gene="fieF"
                     /locus_tag="JNZ73_002170"
     CDS             complement(9708..10610)
                     /gene="fieF"
                     /locus_tag="JNZ73_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HAY8732448.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(10758..11258)
                     /gene="cpxP"
                     /locus_tag="JNZ73_002171"
     CDS             complement(10758..11258)
                     /gene="cpxP"
                     /locus_tag="JNZ73_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HAY8732449.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            11408..12106
                     /gene="cpxR"
                     /locus_tag="JNZ73_002172"
     CDS             11408..12106
                     /gene="cpxR"
                     /locus_tag="JNZ73_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HAY8732450.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            12103..13476
                     /gene="cpxA"
                     /locus_tag="JNZ73_002173"
     CDS             12103..13476
                     /gene="cpxA"
                     /locus_tag="JNZ73_002173"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HAY8732451.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTKLLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(13582..14256)
                     /gene="yiiM"
                     /locus_tag="JNZ73_002174"
     CDS             complement(13582..14256)
                     /gene="yiiM"
                     /locus_tag="JNZ73_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HAY8732452.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(14405..15388)
                     /gene="kdgT"
                     /locus_tag="JNZ73_002175"
     CDS             complement(14405..15388)
                     /gene="kdgT"
                     /locus_tag="JNZ73_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HAY8732453.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAIIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(15648..16268)
                     /gene="sodA"
                     /locus_tag="JNZ73_002176"
     CDS             complement(15648..16268)
                     /gene="sodA"
                     /locus_tag="JNZ73_002176"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HAY8732454.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            16553..17587
                     /gene="rhaT"
                     /locus_tag="JNZ73_002177"
                     /pseudo
     CDS             16553..17587
                     /gene="rhaT"
                     /locus_tag="JNZ73_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(17584..18522)
                     /gene="rhaR"
                     /locus_tag="JNZ73_002178"
     CDS             complement(17584..18522)
                     /gene="rhaR"
                     /locus_tag="JNZ73_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HAY8732455.1"
                     /translation="MAFCNNANLLNVFVRHIANNQLRSLAEVATVAHQLKLLKDDFFA
                     SDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADD
                     KHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVI
                     GQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAAS
                     LKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISD
                     ISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD"
     gene            complement(18506..19342)
                     /gene="rhaS"
                     /locus_tag="JNZ73_002179"
     CDS             complement(18506..19342)
                     /gene="rhaS"
                     /locus_tag="JNZ73_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HAY8732456.1"
                     /translation="MTVLRSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            19630..21099
                     /gene="rhaB"
                     /locus_tag="JNZ73_002180"
     CDS             19630..21099
                     /gene="rhaB"
                     /locus_tag="JNZ73_002180"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAY8732457.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELITLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            21096..22355
                     /gene="rhaA"
                     /locus_tag="JNZ73_002181"
     CDS             21096..22355
                     /gene="rhaA"
                     /locus_tag="JNZ73_002181"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAY8732458.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            22597..23421
                     /gene="rhaD"
                     /locus_tag="JNZ73_002182"
     CDS             22597..23421
                     /gene="rhaD"
                     /locus_tag="JNZ73_002182"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAY8732459.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLAVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            23431..23745
                     /gene="rhaM"
                     /locus_tag="JNZ73_002183"
     CDS             23431..23745
                     /gene="rhaM"
                     /locus_tag="JNZ73_002183"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAY8732460.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            24046..>24112
                     /locus_tag="JNZ73_002184"
     CDS             24046..>24112
                     /locus_tag="JNZ73_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="with FrvBRX is part of the
                     phosphoenolpyruvate-dependent sugar phosphotransferase
                     system involved in the transport and phosphorylation of
                     sugars; possibly responsible for the sugar specificity of
                     the system; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAY8732461.1"
                     /translation="MAALTASCIDLNIQGNGAYSVL"
BASE COUNT         5984 a         6296 c         6132 g         5700 t
ORIGIN      
        1 tacgaaccag cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa
       61 cgccattgtc tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg
      121 tttagaagat tatatcagca gcaataccgg tctggtgatt gacccgtact tttctggcac
      181 caaagtgaag tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga
      241 attgctgttt ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca
      301 tgtgaccgat tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg
      361 ggacgacaaa atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg
      421 ttcttccgaa gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat
      481 ctccgggatc gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg
      541 gatggcgaag aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc
      601 ggtgaaatca gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa
      661 ctatgcgttg gaaggtgcgg tgtttatggc aggcacatcc attcagtggc tgcgcgatga
      721 gatgaagttg attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac
      781 caatggtgtg tatgtggttc cggcatttac cgggctgagt gcgccgtact gggacccgta
      841 tgcgcgcggg gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc
      901 gacgctggag tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc
      961 tggtatccgt ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat
     1021 gcagttccag tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac
     1081 cgcattgggt gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga
     1141 gctgcaagag aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg
     1201 taattaccgt tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca
     1261 cgacgagtaa tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct
     1321 tacgcgtctt atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca
     1381 cgccgcatcc ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa
     1441 taatcccacc cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta
     1501 tgagacgaga acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct
     1561 acaaatggtt aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc
     1621 gtattatgct caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg
     1681 acgaagcacc gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag
     1741 atattgctgt tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc
     1801 tggcggtgct ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg
     1861 agaagctgat tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg
     1921 ataacctgcg caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga
     1981 ttctggctaa accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg
     2041 tatttgctat tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg
     2101 aagttgacgt gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga
     2161 tccgcgcatt agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg
     2221 acaacgaaga gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca
     2281 tcgaagccgg taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct
     2341 ctgccaccgg tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg
     2401 cgactaccga aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca
     2461 tccactatct gaatcgcaaa gacccggaaa tgcaagtgca cattctctga gtgatttgat
     2521 cgattgagcc ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg
     2581 caagtcaatc aaaacaggag aaaaaaatgg ctgattgggt aacaggcaaa gtcactaaag
     2641 tgcagaactg gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta
     2701 ccgccgggca atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct
     2761 actcctatgt taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg
     2821 atggcaaatt aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta
     2881 gcgaagcagc tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc
     2941 tggcaaccgg tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag
     3001 atcgcttcaa aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt
     3061 tgccactgat gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg
     3121 tcagtcggga aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg
     3181 gggaactgga aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt
     3241 gcggcaatcc acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga
     3301 cgaaacattt acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta
     3361 cttatcgata aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata
     3421 tgccggcatc aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg
     3481 ctctttttgc ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt
     3541 tgtcttcgga cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctc
     3601 caacaattga agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa
     3661 gccatcatca atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc
     3721 tttattcacc agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata
     3781 agccatgttt aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca
     3841 aaaataatca tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc
     3901 tccagatcct taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg
     3961 gcaagctctt tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc
     4021 gaaaacttac tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct
     4081 ttagtactgc atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg
     4141 tcgattacga cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat
     4201 ggattaccat tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat
     4261 tgtcatttgc atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa
     4321 aggttaatta cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata
     4381 tttattttcg ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat
     4441 caacgtcggg acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt
     4501 acccgccagc agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg
     4561 atcatgcaag cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa
     4621 cgccgcggtc tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc
     4681 tgcgaaccac aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga
     4741 gaataaccat tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta
     4801 cccttttgac aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact
     4861 gtcaatgaag ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac
     4921 aacggcgcat gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc
     4981 ccctactttc gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag
     5041 cctgccactg aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg
     5101 tgctgcaagt aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt
     5161 aaaaatcaaa agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc
     5221 gaaagcgaaa gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac
     5281 caaaacacaa agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta
     5341 ttacaccgag acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct
     5401 gaccttcgct gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa
     5461 atgacaaaaa gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc
     5521 cctgcggggc ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat
     5581 cctttagtga tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt
     5641 tctaacctgc gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg
     5701 gaaatgtaca tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg
     5761 caaaacgtgg acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg
     5821 aaagacatcg gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa
     5881 gagtctgacg aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg
     5941 gttctgtgca tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc
     6001 gcacgtcaga tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt
     6061 atcgcttatg aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca
     6121 caggctgttc acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa
     6181 caagtgatca ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct
     6241 cagccagaca tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca
     6301 gtgatcgtta aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc
     6361 atatccggca cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc
     6421 ctggatttcc tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa
     6481 cacaaacgaa ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg
     6541 cgcctcaggc acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc
     6601 cgttacccga cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg
     6661 gctgctgatg ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga
     6721 gatatgaata tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac
     6781 cgcgcgatcg ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc
     6841 caaccaaaag aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat
     6901 acgatacgtt gacatcaaca gatattgcag tggaccatta agatctttta aaaccacgta
     6961 tccggcattg ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg
     7021 caaacattcc tcaaggacac tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca
     7081 gtaaccaata ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc
     7141 ttttttcatc tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac
     7201 aaataaaaaa cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc
     7261 gtttgctgat ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc
     7321 agccgccgaa ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact
     7381 ttttcgctac ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt
     7441 ctggcgagta gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga
     7501 ccactttatc gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga
     7561 tttcgaattt atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga
     7621 tcagtacatc gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat
     7681 ccagaacttc gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt
     7741 tgttgttgtg atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag
     7801 agcttttcgg attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat
     7861 ggatctgctt cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg
     7921 agttatccgg cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt
     7981 ccacgtcata ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg
     8041 tcgcttgttt acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct
     8101 gtttccagtg ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat
     8161 atgaaacgtt aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg
     8221 ttaaccctac gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa
     8281 agcgtgccag aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg
     8341 gtatatgttg agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg
     8401 ctttgtccat gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc
     8461 aataaattgc agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct
     8521 gtcatcggtt tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc
     8581 gcgcagtcca gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg
     8641 tcgtggtgaa ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc
     8701 agcagatcga tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga
     8761 gaaccaccgc gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc
     8821 tcgatgaaat gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg
     8881 tgttttttac ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat
     8941 tcaacttccg gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga
     9001 tcgccacaat aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg
     9061 tcacgcagac ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg
     9121 tagtcagtgc ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc
     9181 atttcggtca gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg
     9241 tcgataccac gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat
     9301 tccgggaaac gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg
     9361 taacggtcta gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc
     9421 attacttcca gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg
     9481 cctggcgcat caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa
     9541 ctttggaatg caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa
     9601 cgaatgtgca aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg
     9661 gcggtatttt atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata
     9721 gaccgtttac cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat
     9781 cccggaaaac gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc
     9841 aaaggcagag agtcttccat ttccaaatgg atctgaataa agcgggtcgg ccctgactgc
     9901 cgcgtgcgaa gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt
     9961 tcttgccgtt cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc
    10021 atgcgtaacg cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg
    10081 cgatgccagc cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca
    10141 tcagactggt aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc
    10201 caccgctgaa acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc
    10261 ccgacgcctg gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac
    10321 aggaatagtg ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct
    10381 ttaccgtgac caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt
    10441 aaattcgtca acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc
    10501 accgacccgg tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc
    10561 gtcgcagcaa tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc
    10621 gctatcaact gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc
    10681 aaattgagga taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc
    10741 aaggaaaaca gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat
    10801 gaactttttt gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg
    10861 tttaaaaccg cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc
    10921 atctcaacct gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg
    10981 ttttcatcaa aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta
    11041 acaggaggct gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt
    11101 tcggttaaac ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca
    11161 cccggatgcc agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc
    11221 agcgttgagg ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct
    11281 gtcgcgattc aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta
    11341 aaacaacgta aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat
    11401 ttaaacaatg aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa
    11461 ggagctgctc gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct
    11521 tgatcttctg gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa
    11581 tggtatcgac acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac
    11641 ggcgcgcggc agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct
    11701 cccgaaaccg tttaatgatc gtgagctggt ggcacgtatt cgcgcgatcc tgcgccgttc
    11761 gcactggagc gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc
    11821 cttagtgctg aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac
    11881 cggtactgag tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg
    11941 tgaacattta agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga
    12001 tatgcacatt tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt
    12061 taaaaccttg cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc
    12121 gcgcgcatct tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg
    12181 ttacccaagc tcgattcacg ccagatgacc aagcttctgg atagcgaaca gcgtcagggg
    12241 ctgatgattg agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg
    12301 tggtggcggc gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta
    12361 ttggtgacca ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt
    12421 aactttattg gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg
    12481 gaactggtcg gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt
    12541 cgtccggcca gcagttctca atccgatttc attaacttac tgtttgaccg cccgctatta
    12601 ctgctgattg tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg
    12661 gcaaaaccgg cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc
    12721 cagcacccgg aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac
    12781 cagatggtca ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc
    12841 tctcacgagc tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt
    12901 cgtagcggtg aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc
    12961 atgatcaacg atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa
    13021 accatcaaag ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag
    13081 caaatgggca agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat
    13141 ccgaacgccc tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat
    13201 acgaagattg aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac
    13261 gatggtcctg gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc
    13321 gatgaagcac gcgatcgtga atctggcggt acaggattgg ggctggcaat tgttgaaacc
    13381 gccattcagc agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg
    13441 ctggtgattt ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa
    13501 ggcgtttacg ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt
    13561 atcaggccta cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt
    13621 gccgctcagt cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga
    13681 gagtaaacgg tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc
    13741 ctgcacggtc acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt
    13801 ccctggcgct atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc
    13861 tatatcgctg atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac
    13921 ctgaattaat gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag
    13981 accgtcggtt gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg
    14041 aaattcccgc acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg
    14101 cccgccgtga acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat
    14161 caactcacca tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg
    14221 aatcttgcct gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt
    14281 ccccattaga ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc
    14341 aggaactcaa tagcaaaccg gacgaacgtt agttcatccg gctgtgggga ttaaggaagc
    14401 aggtttattt caccgtacct aaaatttcga ttttcgctgc tctggctttg actttacgtg
    14461 accagataga ggtaagaatt ggcaccagaa tcgaagtcac aatgaccgcc gtcgctacca
    14521 gcgaagttgc tgccggagcc atcggtttaa acgcaggcac catttctgca atcagcacag
    14581 gtgtcgctac cgcggcccct gcggaactgg aagcggcaat tccggcagtg ccatcgccac
    14641 cgccaatcaa tttatcggcg ataatcaacg gaataccggt cacgataatt attgccacac
    14701 ccaacaggat ccccagcaaa ccagtctggg caatcacagt caaatcaatg gtattgccca
    14761 gcgcgaaggc aaagaatgga atcagcgttt gcaccgcttt gctgaaaaat tctcgtaatt
    14821 cagggtcaag attccccagg gcaaagccca ccaggaacgg taatactgcg ccgacgaaaa
    14881 catgcggttc aaacgaggca atcccggcag tgcccagaat aatcatcgtc atgagcggcc
    14941 cggactccaa cgacatcaac acaaatgccc cagcttcttc tttcgtaccg tactgctgca
    15001 tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc gccagcgttg
    15061 aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc gaggcaattg
    15121 ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat ttacgcagta
    15181 ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac accgccagaa
    15241 tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc cccgcccccg
    15301 gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc ccccccggga
    15361 ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt tttaagggct
    15421 gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga cattcattta
    15481 atcaataaaa gtgatcaaaa ccacatcaat tgaaacgctg ttttattttt ataggatcat
    15541 tgtttttatt ttggaattgt tcacaaaaaa accaccgccg ttggcgatgg ttcatttttt
    15601 aagctgatat gcggcctata cgcctcattg cagcaggcgg caaatgatta ttttttcgcc
    15661 gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt gatgtagtcc
    15721 ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag gcccagaatc
    15781 gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg gttagcagta
    15841 gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga accaaagcgg
    15901 gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa gtcacgttcg
    15961 atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt ccagaacagg
    16021 ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc tgctggcagc
    16081 tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg caggctttcc
    16141 agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg gatttccatg
    16201 gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga tggcagggta
    16261 tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc gtaagcagtt
    16321 ggtttattat agttaattaa atgatattga aaatgattat caatgccgca cttttcgtaa
    16381 gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact taatgccgtg
    16441 attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag cacgattttt
    16501 acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga agatgagtaa
    16561 cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag cctgttttta
    16621 cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag tcggtgggat
    16681 tgtttcgtgg attattctgc cgtgggccat cagcgccctg ttactaccgg atttctgggc
    16741 gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg gcgctatgtg
    16801 ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt cgatgggaat
    16861 tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa ttatcaacgg
    16921 caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttactcg gcgttctggt
    16981 ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc gcaagatggg
    17041 cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga tgtgcggcat
    17101 tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg aagccgctgc
    17161 cgcacttggc gtcgatccac tgtatgtcgc tctgccaagc tatgttgtca tcatgggcgg
    17221 cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga aggatttgtc
    17281 gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt tactctcggc
    17341 actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctagggcc acgcccgcat
    17401 tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg tattgtgcgg
    17461 cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc cggtaacggt
    17521 gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca tcggcatggc
    17581 gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc cggtttcccg
    17641 ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg aaatatcact
    17701 gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga cctgccgcag
    17761 atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa cgcgctcact
    17821 gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc tagccgccag
    17881 ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg aatcactggt
    17941 gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag ccatttcgtt
    18001 agcaaacggc acatgctgac tactttcatg ctcaagctga ccgataacct gccgcgcctg
    18061 cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc taaatcccgg
    18121 aatcgctccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa taatattctg
    18181 caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa tataaaagag
    18241 atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat taccgcgcca
    18301 gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt gcggataacg
    18361 gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa
    18421 ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg caatatggcg
    18481 tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc tggcgaatat
    18541 cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga
    18601 atccacagcg ataggcgatg tcagtgacgc tggcctcgct gtggcgtagc agatgtcggg
    18661 ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt tgctgcttaa
    18721 gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca tcccaattca
    18781 cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt gatgcgctgt
    18841 tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc ataaacaaga
    18901 tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa
    18961 ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac ggatactgcc
    19021 catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat cgatccggcg
    19081 agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga tgccgatcat
    19141 gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca ttaaacacat
    19201 gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga tgttcaggaa
    19261 aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttacaagac ggaaaaaaat
    19321 ccacactacg taatacggtc atactggcct cctgatgtcg tcaacacggc gaaatagtaa
    19381 tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa aacgtcgatt
    19441 tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac cacaattcag
    19501 caaattgtga gcatcatcac gttcatcttt ccctggttgc caatggccca ttttcctgtc
    19561 agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga aattcagcag
    19621 gatcacatta tgacctttcg caattgtgtc gccgtagatc tcggcgcatc cagtgggcgc
    19681 gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga aatccatcgt
    19741 tttaacaatg ggctgcatag ccaaaacggt tatgtcacct gggatgtgga tagcctggaa
    19801 agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat cgatagcatt
    19861 gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca gcgtgtgggc
    19921 ctgcccgttg cttatcgcga tagccgcacc aatggcctga tgccgcaggc acaacagcaa
    19981 ctcggcaaac gcgatattta tcagcgtagc ggcatccagt ttctaccctt caatacgctt
    20041 tatcagttgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat tgctcacgct
    20101 ctgctgatgc cggattactt cagttatcgc ctgaccggca agatgaactg ggaatatacc
    20161 aatgccacca ccacacaact ggtcaatatc aatagcgacg actgggacga gtcgctactg
    20221 gcgtggagcg gggccaacaa agcctggttt ggtcgcccga cgcatccggg taatgtcatt
    20281 gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc cagccatgat
    20341 accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgccgctta tctctcttct
    20401 ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga cacggcgctg
    20461 gcagccaaca tcaccaatga aggcggggcg gaaggtcgct atcgggtgct gaaaaatatt
    20521 atgggcttat ggctgcttca gcgagtgctt caggaacggc aaatcaacga tctcccggcg
    20581 cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc caatgacgat
    20641 cgctttatca atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg ggaaacggcg
    20701 caaccgatcc cgggaagtga tgctgaactg gcgcgctgta ttttcgacag tctggcgctg
    20761 ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg gtgaagattt ctcgcaactg
    20821 catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgcgc cgatgcctgc
    20881 ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat cggcatcgag
    20941 ttaattacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt cagcaccacc
    21001 gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta tgtggcgcag
    21061 attcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg gaacaggcct
    21121 gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag gcgctgcgcc
    21181 aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt tccggttttg
    21241 aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat ccgggcaaag
    21301 cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg attccggggc
    21361 cgaaacggct taatttacat gccatctatc tggaatcaga tacgccagtc tcgcgcgacc
    21421 agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat cagctcggtc
    21481 tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt acgctctcgc
    21541 atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc cgtcgcgttt
    21601 cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg atcccggatg
    21661 gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg gcagcactgg
    21721 atgaggtgat cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt gagagcaaat
    21781 tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac atggggtatg
    21841 ccaccagccg ccagactgcg ctgtgcctgg acgccgggca cttccacccg actgaagtga
    21901 tttccgacaa gatttccgcc gccatgctgt atgtgccgca gttgctgctg cacgtcagcc
    21961 gtccggttcg ctgggacagc gatcacgtag tgctgctgga tgatgaaacc caggcaattg
    22021 ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt gacttcttcg
    22081 atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg aaaaaagccc
    22141 tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg gcgggcgatt
    22201 acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgtggcag gcggtctggg
    22261 aaatgtattg ccaacgtcac gatacgccag caggtagcga atggctggag agcgtgcggg
    22321 cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg gcacaagcgc
    22381 cttatccggc ctacgggtcg aatgtccatg cgggctgtca ccctcacccc ggccctctcc
    22441 ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa caccgctccg
    22501 gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc cgcaccatgt
    22561 ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac ccagtcctgg
    22621 tttgtccagg gaatgatcaa agccaccacc gatgcctggc tgaaaggctg ggacgagcgc
    22681 aacggcggca acctgacgct gcgcctggat gacgccgata tcgcgcttta tcacgacaac
    22741 ttccacccac aaccgcgcta tattccgctc agccaaccca tgcctttact ggcaaatacg
    22801 ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagctcga tcctgcagct
    22861 aacttaggcg tcgtaaaagt cgacagcgat ggcgcgggct accatattct ctgggggtta
    22921 accaacgaag ccgttccaac ttctgaactt ccggctcact ttctctccca ctgcgagcgc
    22981 attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac caacctgatc
    23041 gccctcacct atgtactgga aaacgacacc gcggtcttta ctcgccaact gtgggaaggc
    23101 agcaccgaat gtctggcagt attcccggat ggcgttggca ttctgccgtg gatggtgccc
    23161 ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc gctggtgttg
    23221 tggcccttcc acggtgtgtt cggttccgga ccgacgctgg atgaagcctt tggtttaatc
    23281 gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg cggcatgaaa
    23341 cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt tacaccacta
    23401 gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt catgcaggta
    23461 aaccccgacg cccacgaaga gtatcagcgt cggcataatc ccatctggcc ggaactggaa
    23521 gcagtgttga aatctcacgg tgtgcataac tacgccattt acctcgacaa agcgcgtaat
    23581 ctgctgtttg ccatggtaga gattgaatct gaagaacggt ggaatgcggt agccagtact
    23641 gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa cccagataac
    23701 agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc caatgtgccg
    23761 gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat attacatctc
    23821 ccggtaagtt atatttccct gatacattgt gagtaaatca caaaaataat gaataaccca
    23881 ttaatgattc atgtggttta tttaaataac cattatgtgc attactccgc aaatctgacc
    23941 tttcactctg tctcaaattg tcattgttta ccgacatatc gcctctcatc acttaatcgc
    24001 tccaccatta caattgatta aaatatattt tatggagtcc gattaatggc agctcttact
    24061 gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccgttct ga
//
LOCUS       DADMGC010000057        23694 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.057, whole genome shotgun sequence.
ACCESSION   DADMGC010000057 DADMGC010000000
VERSION     DADMGC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.057"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            580..2508
                     /gene="thrS"
                     /locus_tag="JNZ73_002185"
     CDS             580..2508
                     /gene="thrS"
                     /locus_tag="JNZ73_002185"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAY8732462.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2512..3054
                     /gene="infC"
                     /locus_tag="JNZ73_002186"
     CDS             2512..3054
                     /gene="infC"
                     /locus_tag="JNZ73_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAY8732463.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3151..3348
                     /gene="rpmI"
                     /locus_tag="JNZ73_002187"
     CDS             3151..3348
                     /gene="rpmI"
                     /locus_tag="JNZ73_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAY8732464.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3401..3757
                     /gene="rplT"
                     /locus_tag="JNZ73_002188"
     CDS             3401..3757
                     /gene="rplT"
                     /locus_tag="JNZ73_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAY8732465.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            3880..3924
                     /locus_tag="JNZ73_002189"
     CDS             3880..3924
                     /locus_tag="JNZ73_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HAY8732466.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4207..5190
                     /gene="pheS"
                     /locus_tag="JNZ73_002190"
     CDS             4207..5190
                     /gene="pheS"
                     /locus_tag="JNZ73_002190"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAY8732467.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5205..7592
                     /gene="pheT"
                     /locus_tag="JNZ73_002191"
     CDS             5205..7592
                     /gene="pheT"
                     /locus_tag="JNZ73_002191"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAY8732468.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRIAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7597..7896
                     /gene="ihfA"
                     /locus_tag="JNZ73_002192"
     CDS             7597..7896
                     /gene="ihfA"
                     /locus_tag="JNZ73_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HAY8732469.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7997..8977
                     /gene="btuC"
                     /locus_tag="JNZ73_002193"
     CDS             7997..8977
                     /gene="btuC"
                     /locus_tag="JNZ73_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HAY8732470.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9040..9591
                     /gene="btuE"
                     /locus_tag="JNZ73_002194"
     CDS             9040..9591
                     /gene="btuE"
                     /locus_tag="JNZ73_002194"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HAY8732471.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYQDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9591..10340
                     /gene="btuD"
                     /locus_tag="JNZ73_002195"
     CDS             9591..10340
                     /gene="btuD"
                     /locus_tag="JNZ73_002195"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HAY8732472.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10418..10882
                     /locus_tag="JNZ73_002196"
     CDS             10418..10882
                     /locus_tag="JNZ73_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8732473.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11129..11831
                     /gene="ydiV"
                     /locus_tag="JNZ73_002197"
                     /pseudo
     CDS             11129..11831
                     /gene="ydiV"
                     /locus_tag="JNZ73_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            11895..13298
                     /locus_tag="JNZ73_002198"
     CDS             11895..13298
                     /locus_tag="JNZ73_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HAY8732474.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPD"
     gene            complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="JNZ73_002199"
     CDS             complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="JNZ73_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HAY8732475.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="JNZ73_002200"
     CDS             complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="JNZ73_002200"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HAY8732476.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="JNZ73_002201"
     CDS             complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="JNZ73_002201"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HAY8732477.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16026..18404
                     /gene="ppsA"
                     /locus_tag="JNZ73_002202"
     CDS             16026..18404
                     /gene="ppsA"
                     /locus_tag="JNZ73_002202"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HAY8732478.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18461..20161)
                     /gene="fadK"
                     /locus_tag="JNZ73_002203"
                     /pseudo
     CDS             complement(18461..20161)
                     /gene="fadK"
                     /locus_tag="JNZ73_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707412.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(20164..20457)
                     /locus_tag="JNZ73_002204"
     CDS             complement(20164..20457)
                     /locus_tag="JNZ73_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY8732479.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20454..21743)
                     /locus_tag="JNZ73_002205"
     CDS             complement(20454..21743)
                     /locus_tag="JNZ73_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY8732480.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFIYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21799..22737)
                     /locus_tag="JNZ73_002206"
     CDS             complement(21799..22737)
                     /locus_tag="JNZ73_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAY8732481.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIISLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTQGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(22757..23521)
                     /locus_tag="JNZ73_002207"
     CDS             complement(22757..23521)
                     /locus_tag="JNZ73_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HAY8732482.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5847 a         5780 c         6243 g         5824 t
ORIGIN      
        1 tgcaatctat gaggagactt ataatgagtt ctgataatga tatcgttttg actcataatc
       61 tattcgcctt atatatatct ggaggttttg aggatgatga ttaattatta tcgccagaac
      121 gaaatgtgca cgaaaaattg ttataagaat tgtgtcactt ttatcattgt gatatgacca
      181 cttcgtcatt aatctcatga gcgtttaaaa tactaccgcc aaatcacgca acgaactttg
      241 ctggattcag tttatgctgc tgtaaatccg ctcgcagtaa acctttcaga cgcaccgtga
      301 tgttatcagt ggttctttaa gcgttttgct ggtgtactca ctacaaacga attgcgaatc
      361 gaatcaatgt gaaacggaaa ggtacaatct cccttttttt agtgttaaca tcgttcaacc
      421 gggtggaagg tgagaggtta atgtctttca tttgtgtacc tgattgcgaa ccaatttagc
      481 atttgttggc tagatggttt cgcaatgaac tgttaataaa caaatttttc tttgtatgtg
      541 atctttcgtg tgggtcacca ctgcaaataa ggatataaaa tgcctgttat aactcttcct
      601 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
      661 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
      721 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
      781 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
      841 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
      901 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
      961 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1021 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1081 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1141 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1201 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1261 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1321 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1381 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1441 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1501 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     1561 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     1621 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     1681 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     1741 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     1801 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     1861 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     1921 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     1981 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2041 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2101 gtaatgattc accgcgcaat tctggggtcg atggagcgtt tcatcggtat cctgaccgaa
     2161 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2221 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2281 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2341 cgtcgcgtcc catatatgct ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2401 gttcgcaccc gccgtggtaa agacctggga agtatggacg taaatgaagt gatcgagaag
     2461 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     2521 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     2581 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     2641 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     2701 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     2761 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tagtacagat
     2821 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     2881 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     2941 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tagtcgaatc cttcccaacg
     3001 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3061 caagtagcac tatctgtgga gcctttgggc ttcacaggca ttgttcgcct ttgtttcgtt
     3121 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3181 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3241 cacattctga ctaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3301 tccaaaggcg atctgggctt ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3361 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3421 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3481 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     3541 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     3601 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     3661 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     3721 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     3781 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     3841 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     3901 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     3961 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4021 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagtg cggagaaacc
     4081 ggagcgtact taagtacgtg aggatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4141 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4201 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4261 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4321 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4381 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4441 gaaagcgctg cactgaatgc gcgtctggcg gcggaaacga ttgatgtctc tctgccaggt
     4501 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     4561 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     4621 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     4681 tggtttgacg ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     4741 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gtgtttatcg taacgactac
     4801 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     4861 agctttacca acctgaaagg cacgctgcac gacttcctgc gtaacttctt tgaggaagat
     4921 ttgcagattc gcttccgtcc ttcctacttc ccgtttaccg agccttctgc agaagttgac
     4981 gtcatgggta aaaacggtaa atggctggaa gtgctgggct gcgggatggt gcatccgaac
     5041 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5101 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5161 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5221 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tacgctggcg aatcaaatca
     5281 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5341 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5401 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg cggtgcgcca aactgccgtc
     5461 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     5521 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttt tctgagctgg
     5581 gaatttctga cgatcacagt ggcattatcg aactgcctgc ggatgcgccg attggcaccg
     5641 acatccgcga atacctgaaa ctcgatgata acacgatcga aatcagcgtg acaccaaacc
     5701 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     5761 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     5821 cagtcgaagc gccggaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     5881 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     5941 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6001 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatagcaaaa gagggcgaaa
     6061 cgctggtgct gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6121 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6181 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6241 gtgctcgtcg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6301 cggtactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6361 gtgaggctgg cccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6421 tcactctacg tcgtagcaaa ctggatcgcc tgataggcca tcatattgct gatgagcagg
     6481 taactgacat tctgcgtcgt ctcggctgcg aagtgaccga aggcaaagac gaatggcagg
     6541 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtc gaagaagtcg
     6601 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     6661 gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     6721 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     6781 caggcgttga agccttactg ctggcaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     6841 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     6901 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     6961 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7021 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgaccttgaa tccgttctcg
     7081 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7141 cggggcaatc cgcagcgatt tatctgaaag gtgaacgtat tggttttgtt ggagttgttc
     7201 atcctgaact ggaacgtaaa ctagatctta acggtcgcac tctggtgttc gaactggagt
     7261 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7321 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7381 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7441 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7501 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     7561 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     7621 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     7681 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     7741 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     7801 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     7861 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     7921 atgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     7981 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8041 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8101 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8161 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8221 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8281 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8341 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8401 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8461 ctgcagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     8521 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     8581 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc aggccgatga atatgttagc
     8641 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     8701 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     8761 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     8821 acttcccggc tgcgcgctgg caggggcgag cgcattgctg ctggccgata ttgtagcgcg
     8881 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     8941 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9001 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9061 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9121 ctgttaattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9181 aatattcaaa aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9241 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9301 ggggtgacgt tcccgatgtt cagtaagatt gaagttaatg gcgaaggacg ccatccgctg
     9361 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9421 gcccgtatgg tcagcaaagg ccgtgcaccg ctgtaccagg atgatatttt atggaatttt
     9481 gaaaaattcc tagttggcag ggacggaaaa gtcatccagc gtttttcccc ggatatgacg
     9541 ccggaagatc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
     9601 tgatgcagtt acaggatgtt gcggaatcta cccgcctggg gccgctttca ggcgaggttc
     9661 gggctgggga aatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
     9721 cgcgaatggc cggaatgacc agcggtaagg gaagcattca gttcgcgggg caaccactgg
     9781 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
     9841 cgccgtttgc aatgccggtc tggcactacc tgacactgca tcagcacgat aaaacgcgta
     9901 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggtcgtagca
     9961 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgttgcaaa
    10021 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10081 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgcgctgtgt cagcaaggac
    10141 tggcgattgt gatgagcagt cacgatctca accacacgtt gcgtcatgcg catcgggcgt
    10201 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10261 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10321 tgctgatttc gaccatctga tttcattagt atcccgaaaa tcgggtaatt acgacgctaa
    10381 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10441 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10501 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    10561 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    10621 gacgatgcgc gatcgtttcg atttgcatct gccccgtgaa accaaacaac aagcctctat
    10681 tggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    10741 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    10801 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    10861 ctggcaggcg agacgaatct agtgtgtcga aaaattgacg gcgatgatac atcgccgtca
    10921 atttactcct gaatacagta gtattttgca gacaaaaatt ccgtaaatgg attattcgtt
    10981 tataatcagg ataatctaaa aaaaggcccc ctgaaaaagt ggttaacatg gaacaaatga
    11041 aagttactcc ttctgtaatt gttatgtctt atggcctcat cccttttagc cggatactga
    11101 aaaacatcct tcgagaggga cggttaccat gaagattttt ttggagaatc tttatcattc
    11161 tgattgctat ttcctgccga taagagataa ccagcaactc cttgtcggcg ttgagttaat
    11221 tacccatttc tccagtgaag atggcacggt tcgtatcccg acgagtcggg ttatagcgca
    11281 acttaccgag gagcaacatt ggcaattatt ttcagagcag ttggaattac taaaatcatg
    11341 ccaacacttt tttattcaac ataaactctt tgcctggctt aatttaacgc cacaagtgac
    11401 tacattatta ttagacaggg ataattacgc aggcgaacta ttaaaatatc cgtttattga
    11461 gctgttgatt aatgagaatt acccgcatct caatgaagga aaagataatc gcgatctttt
    11521 gtccttatcg caaatgtatc cccttgtgtt aggcaatttg ggagcaggta atagcacaat
    11581 gaaagcggtt tttgatggtc ttttcacccg cgtaatgctg gagaaaggaa caaatcacgc
    11641 atcgctcctt cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt
    11701 gcatcatcgc ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt
    11761 tccatgctct ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg
    11821 ttcagcgata accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca
    11881 aacagatagg atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa
    11941 cctatacagc actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg
    12001 aactcgctaa cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg
    12061 gcggcgaaac cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt
    12121 tcggcgtctg ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc
    12181 ttgctgatgg cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc
    12241 gaatgggtga tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg
    12301 cgatgcatta tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc
    12361 cagtgtatcg ggaaacggtg gagccaggcg cgatgctgat gcgtgtggca ccaagtcatc
    12421 tgcgctttgg tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt
    12481 tggctgactt tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc
    12541 gtctctggtt taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg
    12601 tcggctttgc acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg
    12661 attacgggcc gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg
    12721 atcatcaagg gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc
    12781 gtctggcgca gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca
    12841 gctatcagca ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca
    12901 tgacggagca aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag
    12961 agcgcagcga ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg
    13021 cgtcaccgct acgtgatgag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt
    13081 atcgggggcg tttgcaacaa gacgaggtta gcgatagtga gcgtcaacaa ctgatgcaaa
    13141 gcgttaaccc agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag
    13201 aaaagggtga tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg
    13261 acagagatga tgactatgtc agtcgtccac ctgactgagg taaacggctg gaagtcagct
    13321 gctcgagtta aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga
    13381 tattcttgcc cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg
    13441 gtaattcgcc ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg
    13501 agttttctgc tatccgtata acgcataaaa attaataata attgatataa caatgattcc
    13561 tattatcaat attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg
    13621 cgcagaaatg cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa
    13681 aggcgagttt ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg
    13741 attgaccgta agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac
    13801 tttccgccat aattccagca atctccgtag agccattgcg gatttgctga caaatatcct
    13861 cacaaacttc taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca
    13921 ccagatgttc aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg
    13981 catgataatt cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg
    14041 tctgatagat ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg
    14101 ctgcgcggat agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta
    14161 ccggacagga gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc
    14221 caatcgcgcc ccaactgatt aaatcagcaa taaactgacc ggtcaccata tcgaggaact
    14281 cagtcgcggt agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca
    14341 gaccgtgatt tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc
    14401 cgacaacagt tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt
    14461 actggttgcg cagcgactgc agacgggtgg cgtactccat tgcagcggtg agatcgtgga
    14521 tcgagcaggg gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa
    14581 ttctgcggcg ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg
    14641 cgagttcggc gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt
    14701 tcattacaag tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca
    14761 tcacgataaa ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg
    14821 ttagcatgat aacaaaaaaa caaaccaata cactagttct ctagtacatt cggcgactaa
    14881 ggcccatgat atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa
    14941 tccacgggat ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact
    15001 gacgaagcga ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt
    15061 ccgggtcgat agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca
    15121 ccagattatc catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca
    15181 ttgccagata cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct
    15241 gagcctgatc cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg
    15301 ccgcaatgcg cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag
    15361 caatcggcgt tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga
    15421 tatcctggca aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg
    15481 agtagaagac cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca
    15541 ctgcccgtgc acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg
    15601 gaaattgtga cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa
    15661 tataaaaaac gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata
    15721 attctatgaa agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata
    15781 aaatgaacct tcattttatt ttttatgaaa acagcatttc atttttatgg tttcgtttat
    15841 accgatggtt tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc
    15901 ttaaaaagta aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggtaaaat
    15961 atgcaaagat aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt
    16021 gttcgatgtc caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga
    16081 atgatgtaga cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt
    16141 ccggaatggg tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt
    16201 ttctggacca aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg
    16261 acgatgttac tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc
    16321 ccttccagcc tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg
    16381 acgaaaacgc ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt
    16441 cctttgccgg tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg
    16501 cagtgaaaca tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc
    16561 agggttacga tcaccgtggc gtggcgctct ccgccggtgt tcagcggatg gtgcgctccg
    16621 acctcgcatc ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg
    16681 tgtttatcac ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg
    16741 atgagtttta cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca
    16801 ccatggggtc gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg
    16861 ttaaaatcga agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag
    16921 tgcaggaact ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata
    16981 ttgagtgggc gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa
    17041 ccgtgcgctc acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta
    17101 tcgccgaagg ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg
    17161 acatcagcga aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc
    17221 cggactggga accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta
    17281 cctgtcacgc ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag
    17341 atgcaacaga acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata
    17401 ccggttacgt ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga
    17461 tgccggatct gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact
    17521 tcgcctgcct gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta
    17581 tgattggcgt ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa
    17641 acgaaatccg cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc
    17701 tgactgaagg gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc
    17761 tctctgattt taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag
    17821 atgaagagaa cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc
    17881 gcgactgctt cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga
    17941 ccaacgttga gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg
    18001 aagaactggc gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt
    18061 gtgaaatccc gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct
    18121 caattggctc aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg
    18181 tgtctgaatt gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc
    18241 gtgccgcgaa gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg
    18301 aagactttgc cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca
    18361 ccgtggtgca aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg
    18421 cgttcagtcg ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga
    18481 catcctgcgt taatcgccgt ataatatctt tccttaacaa aaacttttgt attttatctg
    18541 aggcagtgcg cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga
    18601 cccgtttacg gctaaaaaaa gccaccatct cttccagcga taatgaatga tgcggcgctt
    18661 tctgcacgac ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac
    18721 aggcatcgtg aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat
    18781 tttcgccgcc gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct
    18841 catccatacg gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg
    18901 ttaattcagg ttcatcaaaa taccccataa acacattggg gccacgcgat gtttcttcac
    18961 cttcgcaacc tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg
    19021 cggcagcgta accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg
    19081 catgcggcga actttctgtg gaaccataaa cacttaataa tttaatgctg cgctgctggc
    19141 attcgcgcgc cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg
    19201 aaaggtctgc gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc
    19261 ctagcataca ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa
    19321 tatctaacaa cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc
    19381 ccgttgcgtg accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc
    19441 gcgcgcaata agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg
    19501 gcagaccctc ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta
    19561 tcgccgtggt cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg
    19621 cgggagccag tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg
    19681 gcaggattaa atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg
    19741 cctgacactt attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca
    19801 cggaaaccgc accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag
    19861 gcagttgaaa tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca
    19921 gacagctcgc ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca
    19981 cggcaatttt gtctggcatc gcacgagcgg tctgctgtca gtaatcggcc agtgaagcat
    20041 cgccccatag cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca
    20101 ctttcatttt ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca
    20161 tgattagccg tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag
    20221 aatagtgtta ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa
    20281 atgaatgttt cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt
    20341 acggaattca ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac
    20401 atggaattta ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc
    20461 gcggttgctc ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt
    20521 cccatgatca ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc
    20581 atgatgtcgg ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc
    20641 gggatcttgc gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg
    20701 tattgcgcca gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg
    20761 gcggcagcct gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc
    20821 aggcagaagc ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc
    20881 attgccagac cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta
    20941 atcagcgggc gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt
    21001 tgcgcatggg cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg
    21061 gtatagataa atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg
    21121 gcaccttcct cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata
    21181 acctctttaa caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag
    21241 cggccaagca ttgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca
    21301 tccccagcct gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg
    21361 cctgggataa actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga
    21421 ttacgcaata cggtataaga tgcgtgttgc ggaacatctg gttgctcgcg gtgaaaatcg
    21481 agggtaacgg cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct
    21541 accggcgctg atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc
    21601 ccgccggtca tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc
    21661 ccggctcgtg ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg
    21721 gcatcaaatt tgtcatccga catgcgttat gctcccttcc cgctgcgttt acagcgggag
    21781 atgtggttga aaggggaatt agcggctcaa ctggctaatc agggcaggga cgactttgta
    21841 gatatcgccc accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt
    21901 gatggcgaca atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat
    21961 cccctgcgtc aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc
    22021 acgctccatc cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag
    22081 caccgccgcc agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac
    22141 gccaaccaca cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc
    22201 gcgacagaga atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg
    22261 agaggtatca ctggtgcacg gttcctgaac accgggtgca agggaaatga tcgccagcgg
    22321 gctgttgatc ttttcctgag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat
    22381 gtgaccatcg acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac
    22441 acttaaccgt gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg
    22501 gtgtttatct ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtgc gttgcagcgc
    22561 gtcgttttgc tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg
    22621 gtcggtattt tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag
    22681 ttcagcataa cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt
    22741 ttcttctccg taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg
    22801 cctccggcga atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa
    22861 tgccgaccag ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact
    22921 gatttaccgg ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt
    22981 taatatcgga ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat
    23041 cttcaagcgt gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat
    23101 taatcaccgg aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt
    23161 cgccggaacc ttcaccaaag atcagtaaat cgaagccgat cttttcaatt gctgccgcca
    23221 gcgcttttgc ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc accaaataca
    23281 ggctgtgcgg cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaatgagc
    23341 cgccaacggt cagcgcggct atctcatcgt catctgttgc gagctggctt gcagcttcaa
    23401 tggcattgag atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt
    23461 ctggagtgac aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca
    23521 tgatttctcc tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa atggcctgaa
    23581 aatagtgtgc gatagcggtg cggggggttc gacggagcaa gctgttttcc ttgccaacaa
    23641 tcttgcaaaa aaaatgcgat tgctaaaaca aaagtgcaat ttgcggcaat atag
//
LOCUS       DADMGC010000058        23379 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.058, whole genome shotgun sequence.
ACCESSION   DADMGC010000058 DADMGC010000000
VERSION     DADMGC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.058"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(269..886)
                     /gene="tdk"
                     /locus_tag="JNZ73_002208"
     CDS             complement(269..886)
                     /gene="tdk"
                     /locus_tag="JNZ73_002208"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAY8732483.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQT
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1491..1904
                     /gene="hns"
                     /locus_tag="JNZ73_002209"
     CDS             1491..1904
                     /gene="hns"
                     /locus_tag="JNZ73_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HAY8732484.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2048..2956)
                     /gene="galU"
                     /locus_tag="JNZ73_002210"
     CDS             complement(2048..2956)
                     /gene="galU"
                     /locus_tag="JNZ73_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAY8732485.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="JNZ73_002211"
     CDS             complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="JNZ73_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HAY8732486.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="JNZ73_002212"
     CDS             complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="JNZ73_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HAY8732487.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5281..5739
                     /locus_tag="JNZ73_002213"
     CDS             5281..5739
                     /locus_tag="JNZ73_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HAY8732488.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5789..6631
                     /gene="purU"
                     /locus_tag="JNZ73_002214"
     CDS             5789..6631
                     /gene="purU"
                     /locus_tag="JNZ73_002214"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HAY8732489.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6791..6875
                     /locus_tag="JNZ73_002215"
     tRNA            6791..6875
                     /locus_tag="JNZ73_002215"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6825..6827,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="JNZ73_002216"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="JNZ73_002216"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="JNZ73_002217"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="JNZ73_002217"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAY8732490.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="JNZ73_002218"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="JNZ73_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAY8732491.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="JNZ73_002219"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="JNZ73_002219"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY8732492.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="JNZ73_002220"
     CDS             complement(10156..13899)
                     /locus_tag="JNZ73_002220"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY8732493.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="JNZ73_002221"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="JNZ73_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HAY8732494.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="JNZ73_002222"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="JNZ73_002222"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HAY8732495.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="JNZ73_002223"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="JNZ73_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HAY8732496.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVIFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="JNZ73_002224"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="JNZ73_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HAY8732497.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNFGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQMRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="JNZ73_002225"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="JNZ73_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HAY8732498.1"
                     /translation="MKKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /locus_tag="JNZ73_002226"
     CDS             complement(20438..21133)
                     /locus_tag="JNZ73_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY8732499.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="JNZ73_002227"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="JNZ73_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HAY8732500.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="JNZ73_002228"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="JNZ73_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HAY8732501.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGFLSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23296..>23379
                     /locus_tag="JNZ73_002229"
     CDS             23296..>23379
                     /locus_tag="JNZ73_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrA/LdrC"
                     /protein_id="HAY8732502.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIV"
BASE COUNT         5863 a         6100 c         5628 g         5788 t
ORIGIN      
        1 ttctggggcc gtttttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc
       61 ttcatcatat ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc
      121 acaagtcaac gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgcc
      181 gagaagggta tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct
      241 gctcaccctg tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg
      301 ttaatgagcc gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt
      361 cattaccacc aattaccacc tgctcacctt cgttataagg tctgcctgtt tgatcaagac
      421 gcagcaccat gcttgcttta cggccacaaa aacagatggt ttttaattca accagtttgt
      481 cggaccatgc cagtaagtat tggctgccaa taaataattc acctcgaaaa tcggtacgta
      541 aaccataaca aagtacgggt atatcgagtt gatcgacaac ctccgataat tcatatactt
      601 gttgtctggt taaaaactgg cattcatcaa ccagtacgca atgaattgcc tgctgttcat
      661 gttccgcacg aatctcatca aataatgatg aattttggtt aaataatttt gcaggcgatg
      721 acaaacctat acgcgaactg actttcccgg caccaaagcg atcatcaatt tctgccgtat
      781 atacgacagt gcgcatgccg cgttcctggt aattgtatga agattgcaac aatgctgtag
      841 acttacccgc attcattgcg gaatagtaga aatatagctg tgccatcagc cacaggccct
      901 caatgatatg aaataaagtt ggctggagtt tatcataatt cgtcagtttt tcagctcaac
      961 agtatgcaca gaagtgggta tatgcgttct cccttacgaa gccttgcata atccttctga
     1021 gctatcatta caactgcctc gcttgttata agcgggtaaa tgactgctgg taactattca
     1081 caatctttaa cctgttgcgc aagtaatagc cctctgttga cctccaggag atagtgcaat
     1141 actaagtcca tgctcttatt gcgactgttc tacttttcat cattcgctta atagggaatt
     1201 ctcgtaaaca caactaatac agaagactga aaggtcgtca gcctacgata atctccccat
     1261 aaaatgtgac atgaatcagg aagttttaac ctcacgtgct gcgaaatcat cggtgtaaat
     1321 agggctatat gccgcgtctt ttctggctaa ttttatgaaa agatatttat tggcggcaca
     1381 aaataaagaa caattttgaa ttccttacat tcctggctat tgcacaactg aatttaaggc
     1441 tctattatta cctcaacaaa ccaccccaat ataagtttga gattactaca atgagcgaag
     1501 cacttaaaat tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg
     1561 aaacgctgga agaaatgctg gaaaaattag aagttgttgt taatgaacgt cgcgaagaag
     1621 aaagcgcggc tgctgctgaa gttgaagagc gcactcgtaa gctgcagcaa tatcgcgaaa
     1681 tgctgatcgc tgacggtatt gacccgaacg aactgctgaa tagccttgct gccgttaaat
     1741 ctggcaccaa agctaaacgt gctcagcgtc cggcaaaata tagctacgtt gacgaaaacg
     1801 gcgaaactaa aacctggact ggccaaggcc gtactccagc tgtaatcaaa aaagcaatgg
     1861 atgagcaagg taaatccctc gacgatttcc tgatcaagca ataatctttt gtagattgca
     1921 cttgcttaaa atcccgccag cggcgggatt ttttattgtc cggtttaaga caatttaata
     1981 agaataagct ataaaaaaac ggcgtcgatt gctcaacgcc gtttcgtgga taacaccgat
     2041 acggatgtta cttcttaatg cccatctctt cttcaagcca ggctttaaat tccgtgccaa
     2101 gggtgttatg acgaataccg tattcaacga aggcctgcat gtaacctaat ttattaccgc
     2161 agtcatggct cttccctttc atatgatagg cttccaccgt ttctttttcg atcagcatat
     2221 caattgcgtc ggtgagctga atttcatcac cagctcccgg aggggttttt gccagcaacg
     2281 gccaaatatc cgcgctaagt acgtaacgac ccacaatagc gagattagac ggcgcaacat
     2341 ccgctttcgg tttttctacc acaccaacca tcggtacgct ttcacccggc gctaattcaa
     2401 cgcctttgca atccacaacg ccatatgcgg tcacatcagc aaccggttca accatgatct
     2461 ggctatgacc cgtttcatca aagcggcgga tcatctctgc caggttatcc tgtgacaaat
     2521 cggattcata ttcatccaga ataacatcag gcaaaataac agctaccggt tcatcaccca
     2581 ctaccgggtg agcacacaat accgcgtgtc ccaggccttt cgccagaccc tgacgaactt
     2641 gcataatagt cacgtgcggt ggacaaatag actgcacttc atcaagcagt tgacgtttta
     2701 cacgtttttc cagcattgcc tccagttcaa aactggtatc aaagtggttt tcaatagagt
     2761 ttttagatga gtgtgtaacc agcacaattt cagtaatgcc agccgcaata cattcattca
     2821 cgacgtattg aattaatggc ttatcgacaa gtggcagcat ctctttcggg atggctttcg
     2881 tcgccggcaa catcctggtt cctaatcccg caacggggat aacggctttt ttgactttcg
     2941 tattaatggc agccatttaa attctcctgg actgttcgtg ttttgaacgt gttcatattt
     3001 ctgtatcgca tcccagtata tcagcacccc gaaggggcta agttctgaaa aggaggcgag
     3061 atagcataat taagacaatt acttatacga atggcgacga tagtaccacc agccgacatt
     3121 agcaggtaat gcaaatttag cccgcgttat cgtttgctca ttctgcagac aacatcaagc
     3181 gcagtcgacc accggttccc catatttggc attgccaagc atcgcatcgc tggctcaatt
     3241 gattcaaata agcgttacct aaagtgccta acggaacacc attactgatt tgcacctggt
     3301 gttcgccggt atttaacgtc gcattcagac ccgcagaaac cagaatgaga tttttcagtt
     3361 cgcgatgata atagccaact aatagcggaa actgccccgg cagattggcc tgacgaagta
     3421 aatggtttac ctgcttcaat aacgcgccca actctggcaa ccgttgattt tggtgtgcaa
     3481 gctgttcctg taataatccg ttaaacaatg cgcgtaataa caaggcagca agtacgccat
     3541 tatgtccagc tcgggtgaca tcaaggcaat aaaatgccag atcgttttcc gaaagtgcgg
     3601 caatatcaag caccaggccg ggtttgtccg cggcaaccaa ttgacggtaa ttaacccggc
     3661 aatgggaaat cacctgctga accggcggtt gtagttcctg taataatttc gccgctgcgg
     3721 cagggttatc aaccattgca tcccagtcgc gaaaaagcct ttcctcttcc tcaacgcgcg
     3781 aattaaacat gctgggatag agacaggcaa aaaccatctc gcgcaagcga ttcagatctt
     3841 taactggttt cagcaaaaca tcttcaacgc ccagacgtaa cgctttggca atatctgcca
     3901 tattttcagt ggcagatatc accagaactg gggtctggtc gcctctgtta cgtatatgct
     3961 ccagcagttt aagcccgttc attcgtggca tcgcgatatc acatatcatc aggtctggag
     4021 tgaaacctcc cagcaactca agggcatcca ccccatcagc cgccagtacc gttgtcgctc
     4081 ccaatgagga aaaccatgaa tccagaagcg agcgaaatac ctgctcatct tcaacaatga
     4141 gaatctgttt tccgaccaat ggctgcgtca tgttctctcc cctgaccggc tttactcaat
     4201 agtggcatgc tattgccacc tgcgcctgtc agaattaact taagtgtaga taaaaattct
     4261 ggtcaaatgt tggtgcgtac caacggcaaa agttcgtcca ttttcctttc cactgccagc
     4321 tgtccggccg caatggcagc gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa
     4381 acaggttgaa tcagaatatc gggcggatca cctgccatgc ggttcctttt aaggcggttc
     4441 tccaacacct ggatagacgt ggtcataatc tctgttgccg ttggcgctgt cacagcccga
     4501 cgtgtcgtta tgctgcctaa cctttctttc agacgcgcat gccacggtag agaatcacca
     4561 ttctcgctat tttcttcact gacattaaag gagagcagat cttgttgcat caaatgagca
     4621 tcgtgctgca ggtcgaccgc tatcacaata tcagccccca atgcacgcgt aagagaaata
     4681 ggaattgggt taactactgc cccatcaacc agccagtagc cgttatgtgc aacgggtgcc
     4741 atgagtcctg gaatactgca tgatgcgcga atagcaagat ggagatcgcc ttcagtaaac
     4801 cataattcac gccccgtact taaattggtg gcaacagccg caaagcgacg ggaacaattt
     4861 tcgatctctg tttccggcat tatttcgcga tattgattga agacacgctc gccgcgcagt
     4921 aacccaccgc gctgccagga gaggtccatc aggcgtaaaa cgtcccaata actgaaagag
     4981 gttacccaat cttccagcgc agataatcga tcgcatgcat aggccgcgcc caccagcgaa
     5041 ccaattgaac atcctgcaac gatatcaatt tcaataccca ctttttttag cgcattaata
     5101 acgccaatat gcgaccaacc tctcgccgcg ccagatccca gcgccagccc tatttttatc
     5161 tttctcatta ttcccgcaag attcccctgg aatgcaatcg tagccacatc gctactgctc
     5221 ggttaacata gtgctactca ggcggttaat acgccgttat tgtttccagg gagattattg
     5281 gtgtctcagc tttgtccctg tggtagtgct gtcgagtata gcctatgttg ccacccttat
     5341 gtgtctggtg aaaaggttgc acctgatcca gaacatctca tgcgttcgcg ttactgcgct
     5401 tttgtgatgc aagacgctga ttatttaata aagacctggc atccctcttg tggagcagcg
     5461 gcgttacgtg ccgaattgat gaccggattt gcgcatactg aatggttggg attgaccgta
     5521 tttgaacatt gttggcagga cgccgacaat atcggatttg tcagttttgt tgctcgattt
     5581 accgaaggtg gcaaaacagg tgcaataatt gaacgttcgc gatttttgaa agaaaacggt
     5641 caatggtact atattgacgg tacacgtccg cagtttggtc gcaacgatcc ctgcccttgt
     5701 ggttcaggta aaaaatttaa aaagtgctgc ggccaataat ggttgacggt acggtttagc
     5761 aaacactctc aacaaggttt ttccagcaat gcattcactc caacgtaaag ttctgcgtac
     5821 tatttgtccg gaccaaaaag gtctgatcgc acgtattacc aatatttgct acaagcacga
     5881 gttaaatatc gtacagaaca atgaatttgt tgatcaccgt accgggcgct tttttatgcg
     5941 cacggaactg gaagggattt ttaatgattc caccctgctg gcggatctcg atagcgcatt
     6001 gccagaaggc tccgtgcgtg agctgaatcc tgccggtcgt cgccggatag tgattctggt
     6061 cactaaagaa gcgcattgcc ttggcgattt gttgatgaaa gccaattacg gcggtctgga
     6121 tgtcgaaatc gcggcagtta ttggtaacca cgatacttta cgttctctgg ttgagcgttt
     6181 tgatattccg tttgagctgg taagccatga agggttaagc cgcaacgagc acgatcaaaa
     6241 gatggcggac gccattgatg cttatcaacc tgactatgtg gtgctggcga agtatatgcg
     6301 ggtattaacg ccggaatttg tggcacgctt cccgaataaa atcatcaata ttcaccattc
     6361 attcctgcct gcgtttatcg gcgcacgtcc ttatcaccag gcctatgaac gtggtgtgaa
     6421 gattattggc gcaaccgctc actatgtgaa tgacaatctg gacgaaggcc caatcatcat
     6481 gcaggacgtt attcatgtcg atcataccta cacagctgaa gatatgatgc gcgcaggtcg
     6541 tgacgtcgag aaaaacgtct taagtcgtgc gctatacaaa gtactggcgc aacgcgtctt
     6601 tgtttacggt aatcggacga ttattcttta atcgccagca aaaataactg gttaccttta
     6661 atccgttacg gatgaaaatt acgcaaccag ttcatttttc tcaacgtaac actttacagc
     6721 ggcgcgtcat ttgatatgat gcgccccgct ccccgataag ggagcaggct agtaaaaagc
     6781 attaccccgt ggtggggttc ccgagcggcc aaagggagca gactgtaaat ctgccgtcat
     6841 cgacttcgaa ggttcgaatc cttcccccac caccatcact ttcaaaagcc ccggaattct
     6901 caaacgaatc tgcaatcaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttgaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgtttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca gcaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacatac gacgtttcag caaaagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcaacatacc gaagaagtga ccgacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actggacctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 agccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaaca gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagcgcacg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaattccgtt gctgcccagc tcgcccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccatcgc cattttataa accggcgact
     9181 gctgtgcagc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacgtcca gctgacgctg gtagagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacggac
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt atcaaactca ccgcccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgataat
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcaaagattt
     9901 tacccagcag catggctcgg ttacccatgc gcggttgcag tttgccattg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tggcccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagtgc accgttgctg ttgaagactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgcgga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagcgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttt gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccgttaccg attttctcca tcagcgggcc gatagaggta aagcgttcgt aagttgccgg
    11221 ataatcgcgc tctacgacca taatgtgcgg tgcagttttg cctgggatca ggtcgcattc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcgtgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgctttta gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaggtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtggtcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaagaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagttacag cataacatgc tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcaaatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taacactaat
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcggcatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaagtaat cactacgcag
    14341 gcgatataga aaatcaaaaa tactttcatt gcacctactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatcg cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgcc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gatgaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa accgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttgcggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtta tgacagctcc agtagccctt
    15661 tcggaggcag atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgga gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt agaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccggctat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatatatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aaggggttta
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gcgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttga ctcgagcagc agaacgagac ggacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgcg ctgatgcgtg cacaaaaccg
    16381 agaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgtattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagc catcgcgatt ttacccaacg
    16621 cgcaaacatc agcgggcgca acgaaatggc gatgcttgga actgcgttga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc ggtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaactgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actggtggat accctggttg aacaacttac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggaag cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgtct ggtgccttca catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaggtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tgatattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggctatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc acagacaatc gccagtgatt gcttgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgca tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tgtcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg tacaccaaag cggtaattaa gattcagccc gaggttattt
    18961 tgattttcgc cactttcccc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc aaagttaaac
    19081 aaatcaacac gatcaccaaa atactgttct aggctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgagc tgtcaggtcg tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgataa tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtcctg caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccattatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg attttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgctacaa gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aatgtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgaaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctctgattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgttta
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccttaaca
    20461 caccatccgg ataattctcc gccagtagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcat
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcatgatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcatcagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccagaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagtagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtttt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccaggcc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc ctgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aacgccagct cgctggagac attgctcgac agcatgaatg cccctgttgc
    22561 ctttactggc ttcctggtgg cactgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg ttcagcgcgc gatgaatctg ttctttggtt cagtgttagc
    22681 aacaatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 gcagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggagagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac
    23221 aaaacggggg ctaatcttga aaccagacac ttcaagaata gccgcaaaat gttgtcatta
    23281 caacaaggcg gctatatgac gctcgcgcag tttgccatga ctttctggca cgacctggca
    23341 gcaccgatcc tggcggggat tattaccgca gcgattgtc
//
LOCUS       DADMGC010000059        23233 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.059, whole genome shotgun sequence.
ACCESSION   DADMGC010000059 DADMGC010000000
VERSION     DADMGC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.059"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..305
                     /locus_tag="JNZ73_002230"
     CDS             <1..305
                     /locus_tag="JNZ73_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8732503.1"
                     /translation="GWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRCACCGHTAKENRL
                     SQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
     gene            complement(349..4966)
                     /locus_tag="JNZ73_002231"
                     /pseudo
     CDS             complement(349..4966)
                     /locus_tag="JNZ73_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(5059..5706)
                     /gene="rnt"
                     /locus_tag="JNZ73_002232"
     CDS             complement(5059..5706)
                     /gene="rnt"
                     /locus_tag="JNZ73_002232"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAY8732504.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5809..6216)
                     /gene="gloA"
                     /locus_tag="JNZ73_002233"
     CDS             complement(5809..6216)
                     /gene="gloA"
                     /locus_tag="JNZ73_002233"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAY8732505.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6297..7394)
                     /gene="nemA"
                     /locus_tag="JNZ73_002234"
     CDS             complement(6297..7394)
                     /gene="nemA"
                     /locus_tag="JNZ73_002234"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HAY8732506.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGRAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7431..8030)
                     /locus_tag="JNZ73_002235"
     CDS             complement(7431..8030)
                     /locus_tag="JNZ73_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY8732507.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8133..8372
                     /locus_tag="JNZ73_002236"
     CDS             8133..8372
                     /locus_tag="JNZ73_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HAY8732508.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8421..9317
                     /locus_tag="JNZ73_002237"
     CDS             8421..9317
                     /locus_tag="JNZ73_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAY8732509.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(9572..10081)
                     /locus_tag="JNZ73_002238"
                     /pseudo
     CDS             complement(9572..10081)
                     /locus_tag="JNZ73_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10074..10865)
                     /locus_tag="JNZ73_002239"
                     /pseudo
     CDS             complement(10074..10865)
                     /locus_tag="JNZ73_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(10868..11104)
                     /locus_tag="JNZ73_002240"
     CDS             complement(10868..11104)
                     /locus_tag="JNZ73_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HAY8732510.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            11305..11739
                     /gene="slyA"
                     /locus_tag="JNZ73_002241"
     CDS             11305..11739
                     /gene="slyA"
                     /locus_tag="JNZ73_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HAY8732511.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(11786..12253)
                     /gene="slyB"
                     /locus_tag="JNZ73_002242"
     CDS             complement(11786..12253)
                     /gene="slyB"
                     /locus_tag="JNZ73_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HAY8732512.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            12527..13636
                     /gene="anmK"
                     /locus_tag="JNZ73_002243"
     CDS             12527..13636
                     /gene="anmK"
                     /locus_tag="JNZ73_002243"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAY8732513.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            13734..14063
                     /gene="mliC"
                     /locus_tag="JNZ73_002244"
     CDS             13734..14063
                     /gene="mliC"
                     /locus_tag="JNZ73_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HAY8732514.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            14122..14778
                     /gene="pdxH"
                     /locus_tag="JNZ73_002245"
     CDS             14122..14778
                     /gene="pdxH"
                     /locus_tag="JNZ73_002245"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAY8732515.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            14907..16181
                     /gene="tyrS"
                     /locus_tag="JNZ73_002246"
     CDS             14907..16181
                     /gene="tyrS"
                     /locus_tag="JNZ73_002246"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAY8732516.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            16243..17109
                     /gene="pdxY"
                     /locus_tag="JNZ73_002247"
     CDS             16243..17109
                     /gene="pdxY"
                     /locus_tag="JNZ73_002247"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HAY8732517.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVK
                     AANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVN
                     NVEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMR
                     QPVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRI
                     AKPEHYFSATKL"
     gene            complement(17153..17758)
                     /gene="gstA"
                     /locus_tag="JNZ73_002248"
     CDS             complement(17153..17758)
                     /gene="gstA"
                     /locus_tag="JNZ73_002248"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HAY8732518.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(17864..19366)
                     /gene="dtpA"
                     /locus_tag="JNZ73_002249"
     CDS             complement(17864..19366)
                     /gene="dtpA"
                     /locus_tag="JNZ73_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HAY8732519.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(19977..20612)
                     /gene="nth"
                     /locus_tag="JNZ73_002250"
     CDS             complement(19977..20612)
                     /gene="nth"
                     /locus_tag="JNZ73_002250"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAY8732520.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(20612..21307)
                     /locus_tag="JNZ73_002251"
     CDS             complement(20612..21307)
                     /locus_tag="JNZ73_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HAY8732521.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(21311..21931)
                     /gene="rsxG"
                     /locus_tag="JNZ73_002252"
     CDS             complement(21311..21931)
                     /gene="rsxG"
                     /locus_tag="JNZ73_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HAY8732522.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFGQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(21935..22993)
                     /gene="rsxD"
                     /locus_tag="JNZ73_002253"
     CDS             complement(21935..22993)
                     /gene="rsxD"
                     /locus_tag="JNZ73_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HAY8732523.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(22994..>23233)
                     /locus_tag="JNZ73_002254"
     CDS             complement(22994..>23233)
                     /locus_tag="JNZ73_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY8732524.1"
                     /translation="AKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQAN
                     AEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
BASE COUNT         5708 a         6007 c         6057 g         5461 t
ORIGIN      
        1 agggctggta tgaaatgcgc cgccagcttg agtacaagca gctctggagt ggcggtcagg
       61 tgcttgctgt tccgccagcg tacacaagcc agcgttgcgc gtgctgtggt catacagcga
      121 aagaaaaccg cctgtcacaa agtaaattca gatgccaggt atgtggatat acagcgaacg
      181 ccgatgtaaa tggcgctcgt aacattttag cggcggggca cgccgttctt gcctgtggag
      241 agatggtgca gtcaggccgc tcgttgaagc aggaacccac cgaaatgatt caggcgacag
      301 cttgaacgta gcagggaacc acgtctttca gggcgtggag gatgtcaact atccccaatc
      361 cagcccttgt ggcgaacttg aaaatcccgc ctcacgcagc agcgtaaaca tcggcgtttg
      421 ccggaccgga agatcattca cttctgttag cgtaaagcgc agccttggtt cgcgacgcag
      481 tgcggtggtc agcgcctgga aaatctctgg ggcgagtaat tcctcctttt cctgccagac
      541 cagcattttt ttgccgcctt gcggcagata gagtaacaat ttgccgccag aaacgaccac
      601 cagcgcaccc gcccgacgcg ttggtaccag cgctgccgga tgtgcaggcc agggcagaag
      661 atttccccac acattagccg gatcgttggc ggagagcgcc actggtgtat agtggcgtgt
      721 ttgcgtggct tgtgtcgcca gatcgcgcaa tcggtcaata gtcagacgtt cagcgaattg
      781 cgcgccaccc agaccttcta caaaacgacc tcgcataatt cgcccggagt cttccatact
      841 tcgacaaagt gtttgcatcg atggaaaccc gccagggata ttttcggcta tcaccgcctg
      901 acgactgatg atgccgtagc ggtcgagcat attttccgcc agcgccagca tcctttcggt
      961 atcgtttagt ggctccacct gcaataacga ccagcgtcca gccagatttg gtgtgttgta
     1021 agatacccgc ggcgagacgg gttgtgcata gacaggacgc ccacgacgag cccggtgact
     1081 gcggcgagtt gaggtgcgtg cgttggaact gctgcgggtg agggcgcgta acggtgccca
     1141 aatatcgctg gtaatgacgc cttgccagac cagcgcccat aacgcctctt gcagtgccga
     1201 ggaatcaacc gattcgccga ttttatcgcg tatccgttgg ctgatttgtt gtgcaaacca
     1261 ggctcctccg tcagccagaa cagcgattat cgcttgctgc agcgccgaac gattcgcctg
     1321 atccgcttcg gcgggagtga acgattctgc ggcatatttc tgtagatgca gtgccaccag
     1381 gccgtcatct tcacccagct tttttttgtc ccgaccagat aaccgctccg gttgccagta
     1441 attcatcgag catttctggc gaatagtcgc gtacgcgggc aggcaggatc tggctttccc
     1501 agagtgaggc gggtaaaccg actccggcaa gctgttcgat cacccgcatc acgccatcta
     1561 cgccctcata aacgcctggc gatgttgagg caaagagcgc cgggctacca tcggtggcgg
     1621 gtaatacgcc ctgacgttcc agcagcaatc gcgcataggt cgtggctgca acgggacgcg
     1681 tcgcttctct ggcggcttgc agcgagcgca aacgcagacg acgaaatact tcatcgctca
     1741 cccagatgtc ttgttgcaga ttcatcacca gaccctgttc acgcagttgc tgaagctgct
     1801 cttcgacaat ggcaataccg agactaaact catgagccag ttgttcagcc gtgaccagag
     1861 catgagcccg gagatagcgc agaaagaggt cgcgaagcgg gtaactgact ctatgtaaat
     1921 aaatctctgg caatgactct ggtagtcgta cgccgagggc atcacgcagc ctggcggcat
     1981 catccataca ggcaagacgc tcctgtccgc taatcatcgc agggaagatt cgttttactg
     2041 caagaagatt ttccagatac gacgcaacct cttcactgct gcctgtatgc cgttgcgcca
     2101 ggtcttcaac ggtcattggc cccagttcgc gcagcaggtc gaacaatcct tcttcacctt
     2161 tcgctcttct gccaggagcc agtcgttgca actcttcttc cacctggcga atgacctgcg
     2221 ggtcgagtaa ttcccccgga tcgacctgtc cgagtagatt gcgcagtaac tcgctgtcca
     2281 gcgacagtac ggatgcccgg cgctctgcca gcggggcgtc gctctggtac ataaattccg
     2341 cgacatagcc gaacaataaa cttgtggcga aaggcgaggg cgtagtggtc gttatatcgg
     2401 atatttgaat ttcgccaccg ttcaggcgac gcatcaaacg ttccagtgcg ggaagatcat
     2461 aaacatcttg cagacattcg cgtagggttt cgagaatgac cggaaaatcc ggatatccct
     2521 gagcgatttc cagcaactga ctggcgcgca gccgttgttg ccaaagcggg gtgcgatggc
     2581 ccggcgtgcg gcccggcatt aataatgccc gcgcggcgca ttcgcgaaaa cgggcggcga
     2641 aaagtgccga gctgcctacc gcctcgcgga caatttgcag caacttttct ggttcaaaca
     2701 aaaaaatcgc ggcgtcgggc aatttaccat cagtgtcagg aatgcgcgca acaatgccgt
     2761 catcactggc gaccacagac gcgtcagcgc cccatagggc atgtattcgc ccggcaatcg
     2821 ccagcgccca gggttcatgc acccgtcttc catagggaga gtgcaaaata atacgccagt
     2881 cgccaatttc atcacggcac cgttcgagga ccagatggcg actccccgga acgatgcccg
     2941 tcgcgttgcg ttgctcgtca atcaacccct gaatattggc gttcgtattt tcttctgcca
     3001 gccacggggg aatagtcccg gaaaagaacg cgccatccgc cagtaaatga agaaaatcgc
     3061 cgatcatctc gcctaattca gccggacgtc cgttaccttc accacgccag aaggggagcc
     3121 gggcagaacg acccggagca ggagtcacaa tcacctgatc gcgggtgatt tgctggatcc
     3181 gccatgaggt agcgccgaga gtgataatgt cgttcacccg cgactcatat accatctcct
     3241 catccagttc acccacccgc cgcgaaccgg ccttttcttc accttcgggt aataacacgc
     3301 tatacatgcc acgatccgga atggtgccgc cgctggtaac cgccaacaat tgagcgccag
     3361 gtcgggcggt caatatcccg gtctccctgt tccagaccag tttggggcga aaagcagaaa
     3421 aatcgccaga gggatagcgc ccggaaagca tatccagcgt ggcgtcaaaa acacgtcttg
     3481 gcagatcttt ccacggtgcg gcacggcgta cgcgggagta ccattcgtct acctgtaatg
     3541 catccatcgc cgcggcggca acggtttgct gcgcaaggac gtcgagagga ttatgcggtg
     3601 gtgtcaggtt ttccagcctg cctgcgaaca tacactctac aatgactgcg gaatcgacta
     3661 aatcacgccg ggtacggggg aaaaacagcc ctttagatac accgccaacc tgatgtccgg
     3721 cgcgaccaat gcgttgtaac ccactggcaa cagaaagcgg cgttgccacc tgaatcacca
     3781 gatccaccgc gcccatatca atccccagtt caagactgga ggttgcgacc acgcagcgta
     3841 attcacccga tttcagcgcc tgttcggtga ttgctcgttg ttctttagag acggagccgt
     3901 ggtgtgagcg ggcaataaaa acgtcgctac tttgtacacg gttagaggtt gcgccggagg
     3961 tcgactcgaa atgggccgca tcaacggcga tagacgggga acgctgtaag cgtgcggcgt
     4021 aaagctcatt taatcgtgcc gtcagttttt ccgccagccc gcgtgaatta gtaaagacaa
     4081 tggtcgagcg atggcgcaac acttcatcaa ggatacccgt ttcaatatat ggccagatgg
     4141 agccttcccg gccggcatgg ctgtcttcgc cggtgccgct ggcgaccgat gagacatcat
     4201 ccatattggc gaccggtacg acaattcgta tctgcggatg gcgcattgcg ggcgggttga
     4261 ctaccgtaac cgggcgatcg ccaccaagaa atgctgccac atcgctggct gagcgcacag
     4321 tggcagaaag gccaattcgc tgtgctgagg tgtggagcag cgcatcgagc cgctccagac
     4381 ttaacgccag atgcgcacca cgtttactgc ccgctaccgc gtggacttca tcaataatta
     4441 ccgtttcgac gccgtgtagc gtttcgcgcg cgcgggaggt cagcatcaga tagagtgatt
     4501 cgggtgtggt gatcagaata tccggcggat tacgggtgag tttgctgcgt tcctgtgcag
     4561 gcgtatcgcc agtacggatc cctacgcgaa gattgacttc cgtttcgccg cgccgccgcc
     4621 gttcatcggc aatacccttc aacaggatct gcaagttgcg ctgaacgtcg gtgcccaggg
     4681 cttttatcgg tgaaatatag aggatgcgtg aggttttacg cttatgcgcc tcgcgggtat
     4741 cttcgccgcc ttcgcggaag agccgatcga gggcgtagag aaatgctgcc agcgttttcc
     4801 cggagccagt cggtgcaatc accagcgcat gttcgcctcg cgccgccaca tgccaggttt
     4861 gcgactggac agcggtcggc tgtttaaagg cgcgaagaaa ccagtcgcgg gtcgccggtg
     4921 aaaacacgtc cggcaggagc gatgaagggt ctggattatc tgccattaac gctattcccc
     4981 gtgaatatca tgcttgatgc gtggaatagc ctcactatag atgcctgaat cattgcatgt
     5041 catcaggcat cgactcgatt acacctcttc ggcggcaggt agcggccagc ctcccagacg
     5101 tttccagcgg ttgacgattt cacaaaacag cacagcagtg cgttcggtgt cgtacagcgc
     5161 ggagtgcgcc tgggtgctgt cgaagtccat gccagcggtc tggcaagcct ttgacaatac
     5221 ggtttgtccg agtgccagcc cggccagtgc agcagtgtca aaagtggcga aagggtggaa
     5281 cgggttacgt ttcagcgagg cgcgttctgc ggcggccatc ataaagctgt gatcaaaatt
     5341 ggcgttgtgc gccaccataa tggcgcggtt acagccgctc gctttaatac ctttacgtac
     5401 aactttaaaa atttcgtgca gcgcctcgta ttcgctgacc gcgccgcgat cgggatcgtt
     5461 cgggtcaatg ccgttgaagg cgagggcttc tggttgcaaa tttgcgccga caaatggttc
     5521 gacgtggaaa tgtaatgtgg tgtccggcat cagccagcct tgttcatcca ttttcagggt
     5581 gatggcggca atctcaagca gcgcatcggt tttggcgtta aatccggctg tttcaacatc
     5641 gatcacaaca ggataaaaac cacgaaaacg gtcgcacaga ccggtaagtt gagcgttatc
     5701 ggacatctga ttctcttaat acggataaat tgcagcgcgc attatgacaa atattacttg
     5761 tcgatgcagt aaagatgcgg gcgcgatgag ttcacgcccg gcaggagatt agttgcccag
     5821 accgcgaccg gcgtctttct cttcgattaa ctcaattttg taaccgtccg gatcttccac
     5881 aaacgcgata accgtagtac cgccttttac cggacccgcc tcacgggtca cgttaccccc
     5941 gttttgacgg attttttcgc acgcttcagc ggcgttatct acgctaagcg cgatgtgacc
     6001 ataagcagtg ccgagttcgt atttatccac gccccagttg taggtcagtt caatcaccgc
     6061 ttcttcggtt tccgggccgt agccaacaaa cgccagtgag tatttgtatt ccgggttttc
     6121 gctggtacgc agcagtttca tgcccagcac tttggtataa aaatcgatgg agcgttgcaa
     6181 atcgccaacg cgcagcatgg tatgaagaag acgcatcttt ttatcctcaa aatggctaac
     6241 agattcaaaa tgttgtttta gtatagcggc ggctttacgc cgctcgcaat gttggattac
     6301 aacgtcgggt aatcggtata gccttccgcg ccgccaccgt agaaactttc ggcacgctgt
     6361 gggttaagct cagctttgag ctgcaagcgg gcgaccagat ccgggttcgc aatccagtca
     6421 cgaccaaatg ccaccgcatc aattaaccct ttgccgatca gcgtttcagc tttttctact
     6481 gtgtatgcac ctgcgccgat aatcggaccg tggaaacggg cgcgtacttt ttcgcggaac
     6541 gcatcagtat acggttcacc ccccgcccaa tctggttctg acatatgcag ataagcaatg
     6601 ccgcgtttac ccagttgttc aatcagatac agtgcatcgg cttcttcatt cgggccgtta
     6661 tctgtgttct ggaaagtacc gattggcgaa acgcgaatgc caatgcgatc ggcaccccat
     6721 tcttcaatcc cggcatcgac cacttccagt accaaacgtg cgcgattttc cacgctgccg
     6781 ccgtactgat cggtacgatg gtttgaagaa ggggaaagga actgatgcag caaataaccg
     6841 tgagcagagt ggagctctac cagatcaaaa ccggcttcac gcgcgttagc aatggcctga
     6901 cggaaatcat tgacgatacc cggaatctct tccagttcaa gcgcacgcgg catggatgtt
     6961 tcaacgcgga tcgcccgacc attttcatcg cgcagagaag tacgtgttcc cgcgctaagt
     7021 gctgaaggcg ctaccggtgc ctgaccgcca ggttgcaggc tggcgtgaga aatgcgtccg
     7081 gtgtgccaca gctgcaaggc catatgacca ttttcagcat gaacgccagc ggtgattttt
     7141 ttccatgcgg caatttgctc cggactatgg atgccaggcg cacctgcata tccttttgcc
     7201 tgggcagaaa tttgcgtggc ttcactaata atcaaaccgg cactggcacg ttggcgatag
     7261 tattccgcca tcaacggggt aggaatgtca cccggttcaa tactgcgcag acgcgtcagc
     7321 ggtgccataa aaatacggtt tgccgccgtg atcgcgccca ctttcagtgg ggaatacagt
     7381 ttttcagatg acataaaggc tcccggtcgt ctggtgataa aaaatgcctg ctaaacggca
     7441 ggcgtcgcaa taatgttttt tacatgggcc agcgcgtttt ccagtggctc gaaactgcgc
     7501 gaaattttgg cctgcagatt cgcgccaagc catagtgcgt aaagcacttg tgcctgttgt
     7561 agcggttcgc cacaaaaggt taaacaatgg ttctcacggc cattttccag agcctgcgag
     7621 agcagggcga tcacgccgcg agcaccttta tccatcgcgc tgcgcatatc ttctgacaga
     7681 tcgcacactt cggcagagag ttttactgtc aggcaaccac tgatggttcc atgttggcaa
     7741 aactggttca gtgtttgctg gtaataagcc agtatgcggt cgcggtagtt accttcgccg
     7801 gattgcagca actcagccag tcgctggtga tatgcggcgt aatgacgctc aagcatggca
     7861 acgccaaacg cttctttaga gcgaaagtag tgatagaagg accctttcgg cacttcagcg
     7921 gtttttagta attcgcttag ccccatcccg gtgaatccac gttgcaggca aagttgctcg
     7981 cccgtcgcca agagatgttc gcgagtatca tgttcggtgt gtttgttcat gcgttggagt
     8041 gtagtagacc agtcggtcta gtgcaagcaa agttgctact ttggacatta acagcttcaa
     8101 taatggtaat aatcttatct gaggagggcg aggtggcgga gcaattagag ttctttcccg
     8161 tccagagccc gtgccgggga atttgccagt ctgacgaacg cgggttttgt cgcggctgtt
     8221 ttcgtagtcg ggatgagcgt tttaactgga ataaaatgag cgatggcgaa aaacaagagg
     8281 tgctgcgcct ttgtcggcag cgattaatgc gcaaattacg cgcaaataag ccagcgtcat
     8341 cagacgaacc ggagcaacca tcactctttt gaacctaatc ttgcgtatac tcctctcata
     8401 tctgataaga ggaagcgatt atggttcagc gtattactat tgcgccggaa ggcccggagt
     8461 tttcccgttt tgtgatgggc tactggcgat tgatggactg gaatatgtcc gcccgccagc
     8521 tggtcagttt tattgaagag catctggatc tcggcgtgac caccgtggac catgctgata
     8581 tttatggtgg ctatcagtgc gaagcggcgt ttggcgaggc actgaaactg gcacctcacc
     8641 tgcgtgaacg gatggaaatc gtcagtaaat gcggtatcgc gacgaccgcg cgtgaagaaa
     8701 acgtcattgg tcattacatc actgaccgca atcacatcat taagagcgcc gaacagtcgc
     8761 taattaatct cgcgaccgat catctggatc tgctgttaat ccaccgacca gacccgttaa
     8821 tggatgccga tgaagtggcg gacgcgttca aacatctgca tcagagcggc aaagtgcgtc
     8881 attttggcgt atcgaacttt acgcctgcgc aatttgccct gttgcaatca cgtctgccgt
     8941 ttacccttgc cactaatcag gtggaaatat ccccggtgca tcagccgtta ctgctggatg
     9001 gcacgctcga ccaactacaa caactgcgtg ttcgtccgat ggcgtggtcc tgccttggtg
     9061 gtggtcgtct gtttaatgat gattatttcc agccgctgcg tgatgaactg gctgtggtgg
     9121 cggaggagtt aaacgcgggc tcgattgaac aggtggttta cgcctgggta ttacgtttac
     9181 cgtcgcagcc gctgccaatt atcggctcag gtaaaattga gcgcgtacgg gcagctgtcg
     9241 aagcagaaac actgaaaatg acccgtcaac aatggtttcg tatccgtaaa gctgcactgg
     9301 ggtacgacgt accgtaagca cttttaggaa tagccgccgt tcaaaaatgt gtcactggtt
     9361 tacacttatt caggaatgca caatgaacgg aggtcctatg aaacgtttta gtctggccat
     9421 tctggcgctg gttgttgcga ccggcgcaca agctgccagt gaaaaagtcg agatgaacct
     9481 cgtcacgtcg caaggggttg ggcagtcaat tggtagcgtc accattactg aaactgataa
     9541 aggtctggag ttttcacccg atctgaaagc attacccccc ggtgaacatg gcttccatat
     9601 tcatgccaaa ggaagctgcc agccagccac caaagatggc aaagccagcg ccgcggaatc
     9661 cgcaggcggg catcttgatc cacaaaatac cggtaaaatt aaccatcgga aaggcgatga
     9721 tggcagccgt gtagcccgcc agttgaaatg catacgcgac gttattcgtg aagtgcgcac
     9781 aggcctaggt acaaaagcca agccacgccg acatgctcaa tagaaaaaac cacggctcat
     9841 tgagcgtatg tccggcaaga agcagtgccg caatggctcc cagtaaactg ccagcgatgc
     9901 gtccgaggct tttgctgatg acaccgccaa cggtgggaaa gctaaccact gcagccgagg
     9961 tcatcgccca atagggttca tccagattca aataataggc aaccgtcagc gccagacaca
    10021 tggcaatggt attgcgtaac gcataacgcc attgcgccag cgtggccctg aaccagcgca
    10081 aagcggacgg gaacgcgttg ggcgagacga acccagggga cgttaggttt cacatccgga
    10141 ataaggcttg agtcactttc aacgctctga tcataaatcg cccgaccgat actggaaacg
    10201 tgaccctgta acgttttgtt atcactatag agggtaattt gtgctggtgc accttcgcgg
    10261 atatggcgca acttggtttc ttcaaaataa ccaatgacgt aaaacgagtg gctgtcgaca
    10321 agggcaaaca gcggtttccc ggtgtcagcg taatcgccga tgcgggtagt gaggttagtc
    10381 acccatccac tcaccggggc gcgaatttcc gtttgcgcca gttgccattg tgtctgtttc
    10441 agcgtcgcct gtgcggcatt aacactggcc tgcatcgctt taacattgag gttagcggta
    10501 tcgagttctt cggcaaaaat aaaattttgt gagagatgac gacgacgatt cgcttcgtta
    10561 ttggcttttg cgaggtcaga ttgcgctttc gccagctgag cctgtgcgtt cagctcggcg
    10621 atctgaaagg gcgttttatc gatcgtgagc agaagttccc ccgcgttaac cagttggttg
    10681 tcttttatat tcagctcaac aatacgtccc gacacctgtg gggtgataga aacctgttct
    10741 gcgtgtattt tcccgtcgcg tgtccacgga gattgcatgt aataattcca cagccaccat
    10801 ccggcaagaa ccgcaactat cgctacaatg attgttgaga aatacttaat tgttttcatt
    10861 gacatagtta ccacgcaatc agtattgcca gagcaaggca aacgcaaatc gcaaacagcg
    10921 ataaatccat taacaaggga tgccagatgt caccggcgta gatccagcca cgaagcagac
    10981 ggtgtacgac aagccagatg acgaatccaa acgcgaatgc tttgaaaaac ggaggaaaat
    11041 agacggacgc accgaacacc agatcttgta agggcaatcc tgttgcattg agcataaact
    11101 tcacaaagta gattccttta cgaccgaata tattgcgtta gattaataaa tattctttaa
    11161 gtgcgaaaaa tttacgcgca atttctgaaa aatatgtctg acggtaacca aatgcagcaa
    11221 tacatttgtt ttagcaatac aattgctgca cactattcta aaagccgcat aatatcttag
    11281 caagctaatt ataaggagat gaaattggaa tcgccactag gttctgatct ggcacggttg
    11341 gtgcgcatat ggcgtgctct gatagaccat cgcctgaaac cgctggagtt aacacaaacc
    11401 cattgggtta cgttacacaa tatccatcag ttacctccag accagtcgca aattcaactg
    11461 gcaaaagcga ttggcatcga gcagccatca ctggtccgta ctctggacca actggaagaa
    11521 aaagggttaa tttcgcgtca aacttgtgcc agcgatcgtc gggctaaacg tattaaactg
    11581 acggaaaagg cagagccact gatcagcgaa atggaagctg ttattaacaa aacccgcgcg
    11641 gaaatattac atggcatctc cgcagaggaa ctggagcaac tgattacgct catcgcaaaa
    11701 cttgagcata atatcattga gttacaggcc aaagggtgaa atgaaggggg cgtgtggtca
    11761 gtaacctgac cacacgcaaa cttatttagc gcggagaaac ggtcacctga ctgccattgc
    11821 tggccagtac gacacgttgg cccggagaga aacgagtgtt gccttgtttc tgtaccacca
    11881 tgatggtatt accatcgtct ttacgaattt ccagctcgac accctgcgtt ttgttcattg
    11941 cactctgtac gccctgacca gctacgccac ctgcaacagc gcctgctgca gtagccagag
    12001 aacgcccggt tccgccacca acagtattcc ccaggaaacc accaagaaca gcaccgccaa
    12061 ttgcaccgat aacgttggaa tcatcaccgc cctgaatctg taccggacgt acgttaacga
    12121 tggtgccata gctgacattc tgtacttgtt tcgcttcaga agcggtataa acatcccctg
    12181 acagggtgtc gttattaaca caaccgacaa gagacagacc taccattgaa acaaccaata
    12241 cgcgtttaat cactgaaaaa tctcctgttc accatgaaac gccacgcaag catccctcat
    12301 ggtcaaagta tatggcatat gtgaatccat ggttacatgt tctaccaaat atgagcaaat
    12361 cataatgaaa gtaggatcaa ccaattataa acaaaacatt catgatgatt attgatttga
    12421 agataatgtt ttactaccaa cggtaaaaat cggtcattcc gcatggtatt cacgaaaagt
    12481 ttatgtttga gtggtgcgca aacgtcagat tgaaaaagga tgaactatga aatcgggccg
    12541 ctttattggc gttatgtcag gcaccagcct tgatggtgtt gatgttgtgt tggcgacaat
    12601 tgatgaacac cgggtcgcac agctggcaag cttgagctgg ccgatcccgg tgtctctgaa
    12661 acaggctgta ctggatattt gccagggcca gcagcttaca ctttcgcagt ttggacagct
    12721 tgatactcaa ctcgggcgac tttttgctga tgcggtcaat gccttgctta aggaacaaaa
    12781 cctgcaggcg agagatatag ttgcgatcgg ctgtcacggt caaaccgtct ggcatgaacc
    12841 gacgggcgtg gcaccacaca ctttacagat tggcgataac aatcaaattg tggcacgcac
    12901 cggaattacg gttgtcggtg attttcgccg tcgcgatatt gccttgggag gacaaggcgc
    12961 accgctggta cctgcgttcc atcatgcctt gctggctcac ccaaccgagc gacgaatggt
    13021 gctcaatatt ggcggcatcg ccaatctgtc actgctcatt cctgggcagc cggttggcgg
    13081 ctacgatacc ggtcctggta acatgctgat ggatgcctgg atctggcgtc aggcaggtaa
    13141 accttacgat aaagatgccg agtgggcacg ggcgggtaaa gttattctcc cactgctgca
    13201 aaatatgctc agcgacccgt atttctcgca acctgcaccg aaaagcaccg gacgcgaata
    13261 ctttaactac ggctggctgg agcgccattt acgccatttt ccgggtgttg atccccgtga
    13321 tgttcaggcc acactggcag aactcaccgc cgtgaccatt tctgaacaag ttttgttgag
    13381 cggtggctgc gaacgattga tggtatgtgg tggaggtagt cgtaatccgc tactcatggc
    13441 gcgtctggcg gtattactgc taggcacaga agtcaccacc accgatgccg ttggcattag
    13501 tggcgatgac atggaagcat tggctttcgc ctggcttgcc tggcggacgc tggcgggatt
    13561 accaggaaat ctgccttccg tcactggcgc aagccaggag acggtactgg gggctatttt
    13621 ccccgccaac tcgtgatatc agaattaact gaattttttc ttatcgtgtc tgccgttaca
    13681 cttggaagat taaggagggt atttgccctc cggaccagga aagtcttcgg gatatgacta
    13741 tgaaaaaact gttaattatc attttgccgg tccttctctc cggttgtagc gcctttaatc
    13801 aactggttga gcgaatgcaa accgatacgc tggaatacca gtgtgatgaa aaaccgttga
    13861 cggtcaaact gaataatccg cgccaggaag tcagttttgt ttacgataat caactactgc
    13921 atctcaaaca gggcctttca gcctctggcg cgcgttactc cgacgggatc tatgttttct
    13981 ggtcgaaagg tgaggaagcg actgtctata aacgcgaccg catcgtcttg aataactgtc
    14041 agttacaaaa tccacagcgt tgagattttt ccaggggcgg cgcacaatag cgtcacccac
    14101 tgacaatccg taaagaaaac catgtctgat aacgacgaat tgcagcaaat cgcgcatctg
    14161 cgccgtgaat acaccaaagg cgggttacgc cgccgcgatc ttcccgccga tccattaacc
    14221 ctttttgaac gttggctctc tcaggcttgt gaagccaaac tggcggaccc taccgcgatg
    14281 gtggtcgcta ccgtggatga acatggtcag ccttatcagc gcatcgtttt actcaaacat
    14341 tacgacgaaa aaggcatggt gttttacacc aacctcggta gccgtaaagc acatcaaatt
    14401 gaaaataatc cgcgcgttag cctgctgttc ccgtggcata cccttgaacg ccaggttatg
    14461 gtgatcggta aagcagaacg actttcgact ctcgaagtga tgaaatattt tcatagccgc
    14521 ccgcgtgata gccagattgg tgcatgggtt tcgaagcagt ccagtcgcat ttctgcccgc
    14581 ggtatccttg aaagtaaatt cctggagctg aagcagaagt ttcaacaggg cgaagtgcca
    14641 ttgccgagct tttggggcgg ttttcgcgtc agccttgaac agattgagtt ctggcagggc
    14701 ggtgagcatc gcctgcatga ccgctttttg taccagcgtg aaaatgatgc gtggaagatt
    14761 gatcgtcttg caccctgaaa agatgcaaaa atcttgcttt aatcgctggt actcctgatt
    14821 ctggcacttt attctatgtc tctttcgcat ctggcgaaaa gtcgtgtacc ggcaaaggtg
    14881 cagtcgttat atacatggag attttgatgg caagcagtaa cttgattaaa caattgcaag
    14941 agcgggggct ggtagcccag gtgacggacg aggaagcgtt agcagagcga ctggcgcaag
    15001 gcccgatcgc gctctattgc ggcttcgatc ctaccgctga cagcttgcat ttggggcatc
    15061 ttgttccatt gttatgcctg aaacgcttcc agcaggcggg ccacaagccg gttgcgctgg
    15121 taggcggcgc gacgggtctg attggcgacc cgagcttcaa agctgccgag cgtaagctga
    15181 acaccgaaga aactgttcag gagtgggtgg acaaaatccg taagcaggtt gccccgttcc
    15241 tcgatttcga ctgtggagaa aactctgcta tcgcggcgaa caactatgac tggttcggca
    15301 atatgaatgt gctgaccttc ctgcgcgata ttggcaaaca cttctccgtt aaccagatga
    15361 tcaacaaaga agcggttaag cagcgtctca accgtgaaga tcaggggatt tcgttcactg
    15421 agttttccta caacctgttg cagggttatg acttcgcctg tctgaacaaa cagtacgatg
    15481 tggtgctgca aattggtggt tctgaccagt ggggtaacat cacttctggt atcgacctga
    15541 cccgtcgtct gcatcagaat caggtgtttg gcctgaccgt tccgctgatc accaaagcag
    15601 acggcaccaa atttggtaaa actgaaggcg gcgcagtctg gttggatccg aagaaaacca
    15661 gcccgtacaa attctaccag ttctggatca acactgcgga tgccgacgtt taccgcttcc
    15721 tgaagttctt cacctttatg agcattgaag agatcaacgc cctagaagaa gaagataaaa
    15781 acagcggtaa agcaccgcgc gcccagtatg tactggcgga gcaggtgact cgtctggttc
    15841 acggtgaaga aggtttacag gcggcaaaac gtattaccga atgcctgttc agcggttctt
    15901 tgagtgcgct gagtgaagcg gacttcgaac agctggcgca ggacggcgta ccgatggttg
    15961 agatggaaaa gggcgcagac ctgatgcagg cgctggtcga ttctgaactg caaccttccc
    16021 gtggtcaggc gcgtaaaact atcgcctcca atgccatcac cattaacggt gaaaaacagt
    16081 ccgatcctga atacttcttt aaagaagaag atcgtctgtt tggtcgtttt accttactgc
    16141 gtcgcggcaa aaagaattac tgtctgattt gctggaaata atgcattaag tggaaagggg
    16201 gagtgagaaa tcactccccc tggtttttat acagggaaca tgatgaaaaa tattctcgct
    16261 atccagtctc acgttgttta tggtcatgcg ggtaacagtg cggcagagtt tccgatgcgc
    16321 cgcctgggcg cgaacgtctg gccgctgaac accgttcaat tttctaatca cacccaatac
    16381 ggcaaatgga ctggctgcgt gatgccgccc agccatttaa ccgaaattgt gcaaggcatt
    16441 gccattgccg ccattgataa attacacacc tgtgatgccg tattaagtgg ctatctggga
    16501 tcggcggagc agggtgaaca tatcctcggt atcgtccgtc aggtgaaagc agcgaatccg
    16561 caggcgaaat atttttgcga tccggtaatg ggtcatccgg aaaaaggctg tatcgttgca
    16621 ccgggtgtcg cagagtttca tgtacggcac ggtttgcctg ccagcgatat cattgcgccg
    16681 aatctggttg agctggaaat actctgtgag catgcggtaa ataacgtcga agaagcggtt
    16741 ctggcagcgc gcgaactcat tgagcaaggg ccacaaattg tgttggttaa acacctggcg
    16801 cgagctggct acagccgtga ccgttttgaa atgctgctgg tcaccgccga tgaagcctgg
    16861 catatcagcc gtccactggt ggattttggt atgcgccagc cggtaggtgt tggtgatgtg
    16921 acgagcggtt tactgctggt gaaactgctt cagggggcaa cgctgcagga ggccctggaa
    16981 catgtgaccg ctgcagtcta cgaaatcatg gtgaccacca aagcaatgca ggaatatgag
    17041 ctgcaagtgg tggctgctca ggatcgtatt gccaaaccag aacattactt cagcgcaaca
    17101 aagctctgaa atatctcatt tcggcccaca acgacctgtg ggccgatggc tattacttta
    17161 agccttccgc tgacagcgcg tcttgtactt ccggacgttc agccatacgt tgcataaatg
    17221 ccgcaatgtg ctctaaccct tccagattca gtttcaccgc gtatgcccag cgcagaaccg
    17281 taaacagata ggcatcagca attgtaaatc tttgcccgca gatccagtgc tcatccttca
    17341 gtgcctcgtt cacatattgc agcttcttat ccagctgcgc gcgaactgtc ggtttgtact
    17401 cttccggtgt atctgggcga aacagaggtg tgaaaccttt atgcagctcg gtggcgatgt
    17461 aattcagcca ttcgatggtt ttatagcggg aaatactgtt taccggtgcc agcaactggc
    17521 ggtcggggac gctgtcggca agatactgca taatcgcaac gccttccgtc agcaaagtac
    17581 catcgtccag cagcaatgca ggcacctgcc ccttagggtt aacggaaaag taatcgtcac
    17641 cgttttcgag acgttttttc attaaatcca cactgacgag cgtaaaatcc tttccgctct
    17701 cacgcagggt aatatgggaa gcgagagagc aggcgcccgg tttgtagaac aatttcatcg
    17761 gtaactcctt ttgtctgtgg tttaaagtat ggtagtgcgt tgctgaacaa aaaaaaagcc
    17821 gctaacgaaa agttagcggc ttgtaaaaga gtttccctga attttacgct acagtagctt
    17881 tcgccgcttt gtctgcagcg tcatcctgcg tcatgcggtg cagtttaggc gcggtcagca
    17941 gcatcagtac tgcaataacg gcagtagcga caccaatctg caagaatacg cgaccataga
    18001 cttccagtga catcagcggg tcggtaacgt tatccggcac agccatcata cccgcaacat
    18061 aaccaccaat caggtttgca ccggcagtgg tcaggaacca gctacccata atgaagccca
    18121 tcagacgctg cggaacgagt tgtgcaacca ttgccagacc cagaccagag atcatcagtt
    18181 ccccgatgct ctgcaggcca tagcttgcga ccagccagct gacagacacg atacctgcgt
    18241 cagacgcgaa tttcgcaccc agcggcagaa tcaggaacgc accagaacac atcaccatgc
    18301 cgattgcaaa cttggttggc atcggcaggg tatcacccat cttgttatag atagcggcca
    18361 gaatcggact accgatgatg atccagaacg ggttcagtgc ctgatactgc tcaggttcta
    18421 cggccagacc cagaatggag tgctcaacgt tacgaatcgc aaagaagttc agtgacgttg
    18481 gcatctggct gtacagcacg aagaagatga ttgcttcgag catcaggatg aaggcaacga
    18541 tcattttacg acgcgcagca cctttcatcg cgaaagcttc tttaccgaag ataaccacaa
    18601 taccgaaggc aacaacgccc agcgccatac gcgcaacttc ctgattgtgc agtagccagg
    18661 tggcaacagc gatcagcgcc acaacaccaa taatggtcag caacaggtta cggtagttga
    18721 ctggttcaaa gtctggtttt gaaccgtact gtttaaccca gcgttggcag aaggcgaagt
    18781 taacgatagt gatcagcagg cctacaacgc tcaacgcaaa cgcaacactc cagccgtatt
    18841 tcgcggccag ccacggggtg gctatcatag agaagaaaga gccgatgttg acggacatgt
    18901 agtacatggt gaatgcaccg tccagacgtg ggtcgttttt ctcatagcat gtagaaagca
    18961 gagaagacgg gttagcttta aacaggccgt taccgaccgc aatagccgcc atacccatat
    19021 aaacaatacc ggcgtcgtgg ccagaccagg caaccagagc ataaccaatc gccagcacaa
    19081 tagcgccgag cataattacg cgtttagtac ccagtacctt gtcacctaac cagccgccga
    19141 tagcgaccag accataaacc agggcactaa aggaagagaa aagggtgatt gaatccgctt
    19201 cagacatacc cagttgttta accaggtaaa cagccataat tccttgtagg ccgtaataac
    19261 caaaacgttc ccataactca atcgagaaga tgagatagaa cgccttcggt tgtttgaaag
    19321 cgttcaaact gacgctttca gttggttttt ggtttgcagt ggacactttt acctcttttt
    19381 ttacatccca tattaacggg ggtgctttct cgcgttatgt ctaaaaaaca tacgcgcaat
    19441 tgttatagtg ggaggggaaa cggcaggtaa tgttcactat cttgcacttt cagacaagtc
    19501 ctttgtaata atctgttaca tataactgag gcggggaatt caaccattcc gggaaatgtt
    19561 atccagcgtt aaatttttca aaaaacgtaa cggcagattt tttcactgca ttgagagggg
    19621 gatgatggct ttacggagat atgttctgct atttagcgtt tttcctagtg agatagtcca
    19681 gtttctgaaa aatagccagt gtaatgtttt gcaggtcaat atttgttgcg tgtgtaaatt
    19741 taaatcattt taacctgatt cttgcaatgt ttttacattg ccacagcatc gtgatcttga
    19801 tcacgaatga atagaaaatt tcgctattaa aaaaacaatt aacctgcatt tttggttatc
    19861 cgcaaacgga ttacctgatg ataacgctaa gcaatggcat cattgacgtt aaaaagatgt
    19921 tttaaaagag gataaagaaa ggttatcaat ggggtaatcg gtgttacccc ttttcttcag
    19981 atgtcaactt tctctttgta ttcacaaaga tcttcaataa tacaagagcc acagcggggc
    20041 ttgcgggcaa tgcaggtata acgcccgtgc aggatcaacc aatggtggca gtcgacttta
    20101 aactctgccg gaaccacttt cagtagcttt tcttctacct gttcgacgtt tttccccggc
    20161 gcaaattgag tacgattaca aacgcggaaa atgtgcgtgt cgacagcaat agtcggccag
    20221 ccgaatgcag tgtttaatac gacgttggct gttttacgac ctacgccggg cagggcttca
    20281 agcgcagcac gatcttccgg aacctcgcca ttatgttgtt ccagtaatat acggcaggtt
    20341 ttgatgatat tttctgcttt gctgttataa aggccaatcg ttttgatata ggttttcacc
    20401 ccttcaacgc ccagttcaag catcgctgca ggcgtattcg ccaccgggta gagtttcgcc
    20461 gtcgccttat taacactgat atcggtcgcc tgagcggaaa gcagtacggc aatcagcaat
    20521 tcaaaaggcg aactgaaatt aagctcggtg gtgggatgag gattgttctc acgcaggcga
    20581 gtgaggatct ccagacgttt tgctttgttc atcagacatt ccctgtttca ccgtttggca
    20641 atgcacgttc ggcagctgct tcagcacggc gctttttcat tctttcatca atcaggtatt
    20701 ttcctgccag catcagtccc aagccaataa atgcacctgg tggcagcatc gccagcagga
    20761 aaggggagtc ggtgtggaaa atctccacgc gtaatacttt tgcccagcta cctaacagcg
    20821 catctgcacc gtcaaacaac gtgccattgc cgataatttc gcgtagtgaa cccagcacga
    20881 acatggcgca ggttgcgccc gtaccaattg aaaagccgtc cagtgccgaa agcgccggac
    20941 cttttttggc ggcgaaggct tcagcgcggc ccacaacgat acagttagtg acaatcagcg
    21001 gaataaaaat ccctaatgat tgatacaggc caaaggcgta ggcgttgatc agcatctgta
    21061 cagcgctgac caccgaggcg atgatcatca cgtaaatggg aatgcggatc tcggctggcg
    21121 tccagtgacg cagcgtcgaa atggtcaggt tggtcagcgt cagtaccagc gtagtcgcaa
    21181 gtcctaaacc cagagcgtta gtggcagtgg acgtgaccgc caatagcgga caaaggccga
    21241 gcagttggac cagtgcagag ttgtttttcc acaacccctg aacaataacg tctttaattt
    21301 cgctcacgct ttattctcca caggcaggaa gttgagaaag ttgtgccggt aacgtctgag
    21361 cgtacaatcc ggcgcgtttt accgcattaa ccaccgcgcg gggagtaatc gtcgcgccgg
    21421 tgaactggtc gaaatcacca ccatctttct tcaccgccca gtgcgcatca tccgcaccac
    21481 tgattttttt tcccgcaaaa tgggtgagcc agtcagaaag gcgcaattcg attttatcgc
    21541 caagccctgg cgtttcgtgg tgctctgtca cgcgcgtgcc aagtaccgtg ccattaaaat
    21601 cggctcccac cagcagctga atcgcaccgg aatagccatc tggcgcggtt gcttccagaa
    21661 cggcggctac cggtttgtca tcctgtttgg cgatgtaaac ccgatgctca cctttaccta
    21721 actctggcgc agttaccaga tagcaactct gtgcgagcgc attgttatag cgttcggctg
    21781 gcagcacctg accaaataac gccttttgtt gcagactggc ctgttcagca attgtcgttt
    21841 tggtcatctg gttgatggcc gcagttaacc ctgtagaacc cgctgcaaat agtgccagtg
    21901 taatgccgtg ttttcggata gttttcagca tggtttaccc tttgcgatgg ccgtagacgc
    21961 gcggacgcgt gtagtaatcg atcagaggaa ccgtgatgtt cgccagcagg acggcaaaag
    22021 ccacgccgtc aggatagccg ccgaaactgc ggatcaacca gactaataag cccgccagcg
    22081 cgccgaacat cagccgacca cgattggtcg tagaagcagt aactgggtca gtcaaaataa
    22141 agaatgcgcc gagcatagtc gcaccagaca gcagatgaat ttgcggtgct gccagtgttt
    22201 ctggtgagaa caaccagccc aacgttgcgc ataacgccag cgttactaag aagctgaggg
    22261 gaatatgcca gcgaatcgct ttctgccata gcaaccacac gccaccagcc agccaggcga
    22321 gatttaccca ttgccaacca gcgcccgcca gaataccgct gtagatcgga tattgcataa
    22381 tctgttcaac cgaatgacct gcgcggacag aggttttgaa tgtatccagc ggtgtcgcct
    22441 gactaatgcc atcaatacct aagcgtagcg tattcatatc accaccactg gcggtatgac
    22501 cgctgaaaat aacctgtatg gcgtcgataa aaccagggat gttgaccgca atttcatgtg
    22561 gcggtaacca gctggtcatc tgcaccggga aggagatcag taagaccaca taaccaatca
    22621 ttgccggatt aaacgggttt tgccccagac cgccatacaa ctgtttagcg atgatcaccg
    22681 caaacaccgt acccagtacg accatccacc atggcgctag ggggggaata cttaccgcca
    22741 gcaataagcc tgtcagcaat gctgagttat ctttcaacgt tgcggctacc gactgcttgc
    22801 gtagtttgag tacgagagct tcggctaaca gagcactgac cgacgccaac aggatctgaa
    22861 cgagagtacc ccaaccaaaa aaccacagtt gcgctgcgat tcctggcacg gctgcgagca
    22921 acaccaatag cataatgcgc gatgtctggc gctggttatg ggtataaggg gagctagcta
    22981 ttctgaatac catttagtcc tcgtttacaa ccttttgctg ggcggctttt ttggcctgaa
    23041 cgcgggcaat agccgcggca actgcggctt tgcgcggatc gacctgttct tctggttccg
    23101 cattagcctg ttgctgttcc agcttgcgtg ctttggcgcg ggcaatagcg gcttcgacgg
    23161 cagctttgcg cggatcaacc tgttcttctg gctccgcatt ggcctgttgc tgttccagct
    23221 tgcgtgcttt ggc
//
LOCUS       DADMGC010000060        22785 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.060, whole genome shotgun sequence.
ACCESSION   DADMGC010000060 DADMGC010000000
VERSION     DADMGC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.060"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..194)
                     /locus_tag="JNZ73_002255"
     CDS             complement(<1..194)
                     /locus_tag="JNZ73_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HAY8732525.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKKGY"
     gene            complement(335..2368)
                     /gene="metG"
                     /locus_tag="JNZ73_002256"
     CDS             complement(335..2368)
                     /gene="metG"
                     /locus_tag="JNZ73_002256"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAY8732526.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2500..3609
                     /gene="apbC"
                     /locus_tag="JNZ73_002257"
     CDS             2500..3609
                     /gene="apbC"
                     /locus_tag="JNZ73_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HAY8732527.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3871..4152
                     /locus_tag="JNZ73_002258"
     CDS             3871..4152
                     /locus_tag="JNZ73_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HAY8732528.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4445..4987
                     /locus_tag="JNZ73_002259"
     CDS             4445..4987
                     /locus_tag="JNZ73_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8732529.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5068..5742
                     /locus_tag="JNZ73_002260"
     CDS             5068..5742
                     /locus_tag="JNZ73_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY8732530.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5758..8238
                     /locus_tag="JNZ73_002261"
     CDS             5758..8238
                     /locus_tag="JNZ73_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY8732531.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            8254..9287
                     /locus_tag="JNZ73_002262"
                     /pseudo
     CDS             8254..9287
                     /locus_tag="JNZ73_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9369..9707)
                     /gene="rcnB"
                     /locus_tag="JNZ73_002263"
     CDS             complement(9369..9707)
                     /gene="rcnB"
                     /locus_tag="JNZ73_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HAY8732532.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9926..10768)
                     /locus_tag="JNZ73_002264"
     CDS             complement(9926..10768)
                     /locus_tag="JNZ73_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HAY8732533.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTV
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHEHHQDHEHHHDQGHHHHHEHGEYQDAHA
                     RAHANDIKRRFDGREVINWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVS
                     FSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFM
                     GIMR"
     gene            10889..11161
                     /gene="rcnR"
                     /locus_tag="JNZ73_002265"
     CDS             10889..11161
                     /gene="rcnR"
                     /locus_tag="JNZ73_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HAY8732534.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11253..11349
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11384..12172
                     /gene="thiM"
                     /locus_tag="JNZ73_002266"
     CDS             11384..12172
                     /gene="thiM"
                     /locus_tag="JNZ73_002266"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HAY8732535.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12169..12969
                     /gene="thiD"
                     /locus_tag="JNZ73_002267"
     CDS             12169..12969
                     /gene="thiD"
                     /locus_tag="JNZ73_002267"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAY8732536.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            13034..13852
                     /locus_tag="JNZ73_002268"
     CDS             13034..13852
                     /locus_tag="JNZ73_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HAY8732537.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVDELQAFVDGIKETP"
     gene            13904..14650
                     /locus_tag="JNZ73_002269"
     CDS             13904..14650
                     /locus_tag="JNZ73_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY8732538.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14624..15589)
                     /locus_tag="JNZ73_002270"
     CDS             complement(14624..15589)
                     /locus_tag="JNZ73_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY8732539.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIRAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15586..16589)
                     /locus_tag="JNZ73_002271"
                     /pseudo
     CDS             complement(15586..16589)
                     /locus_tag="JNZ73_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16586..17062)
                     /locus_tag="JNZ73_002272"
                     /pseudo
     CDS             complement(16586..17062)
                     /locus_tag="JNZ73_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            17216..18268
                     /gene="fbaB"
                     /locus_tag="JNZ73_002273"
     CDS             17216..18268
                     /gene="fbaB"
                     /locus_tag="JNZ73_002273"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HAY8732540.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVAVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            18576..19430
                     /gene="gatY"
                     /locus_tag="JNZ73_002274"
     CDS             18576..19430
                     /gene="gatY"
                     /locus_tag="JNZ73_002274"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HAY8732541.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            19459..20726
                     /gene="gatZ"
                     /locus_tag="JNZ73_002275"
                     /pseudo
     CDS             19459..20726
                     /gene="gatZ"
                     /locus_tag="JNZ73_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            20736..21188
                     /gene="gatA"
                     /locus_tag="JNZ73_002276"
     CDS             20736..21188
                     /gene="gatA"
                     /locus_tag="JNZ73_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HAY8732542.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            21219..21503
                     /gene="gatB"
                     /locus_tag="JNZ73_002277"
     CDS             21219..21503
                     /gene="gatB"
                     /locus_tag="JNZ73_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAY8732543.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            21507..22785
                     /locus_tag="JNZ73_002278"
                     /pseudo
     CDS             21507..22785
                     /locus_tag="JNZ73_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
BASE COUNT         5905 a         5292 c         5752 g         5836 t
ORIGIN      
        1 tcatacccct tcttcacctt ctccgcaatc agcttcagtt ccgctttttc cgccgccgca
       61 gcatctgaaa aactttttat ctgactttgc cctttggtgc caacttttcc ccaactgata
      121 tgcaactcgt ttccctgttg ctccaccgtc cagaatttat gtgatttttc gtcctgatag
      181 ataaagtgtc tcattctgca tccctgtaat tttatccagt tgaatgcaga tgctaccagt
      241 atttatacgg gttagagaga gacaaaatcg caagaaaaca actttaggaa tttataaaga
      301 aaaggcgctg tcgatgcagc gccttgaagg gggattattt cacctgatga cccggtttag
      361 caccggcatc cgggcttagc aggaaaatat ctttcccgcc aggaccggca gccatcacca
      421 tgccttcaga gataccgaag cgcattttac gtggtgccag gttagccacc ataatggtgt
      481 gacgaccaat cagtgcctgc ggatccgggt aagcagaacg aataccggag aagacattgc
      541 gtttttcacc gccgagatcc agcgtcaggc gcagcagttt gtcagaacct tcaacaaact
      601 ctgcgttttc gatcagcgcc acgcgcaggt caactttggc gaagtcgtca aaggtgatgg
      661 tttcctgaat cggatcatct gccagtgggc cagtaactgg cgcggtagtg gctttcactt
      721 cttctttaga ggcttccacc agagcttcaa cctgtttcat atcgatgcgg ttatacagcg
      781 ccttgaacgg attcactttg tggcccagca gcggttgctg gataccatcc caggtcagtt
      841 ccgtattgag gaaggcttct gcacgctcgg tcagtttcgg cagtaccggc ttcaggtaag
      901 tcatcagcac gcggaacagg ttgatgccca ttgagcaaat cgcctgcagg tcagcatcgc
      961 gaccttcctg ttttgcgact acccacggag cctgctcgtc gacatagcgg ttagccaggt
     1021 cagccagtgc catgatttcg cgcactgctt taccaaattc acggctttcc cacgcttcac
     1081 caatcacttc agcggcatcg gtaaaggttt tgtacagctc agggtcagcc agctcgcttg
     1141 ccagcacacc gtcaaaacgc ttgttgataa agcccgcatt acgggaggcc aggttaacca
     1201 ctttgttcac gatatcggca ttcacacgct gaacgaaatc ttccaggttg agatcgatat
     1261 catcaatgcg cgaagagagt ttcgcagtgt agtagtaacg caggctgtca gcgtcaaaat
     1321 gattcagcca ggtgctggct ttaataaagg tgccgcgaga cttggacatc ttcgcgccgt
     1381 tcaccgtcac atagccatga acaaacaggt tggtcggctt gcggaagttg ctgccttcca
     1441 gcatggcagg ccagaacagg ctgtggaagt aaacgatatc tttaccgatg aagtggtaca
     1501 gctcggcggt ggagtctttc ttccagtatt catcgaagct tacgctgtcg ccgcgcttgt
     1561 cgcacagatt cttgaaagaa cccatgtagc caatcggtgc gtccagccag acgtagaaat
     1621 atttgcccgg cgcgttcgga atttcaaaac caaagtatgg cgcgtcgcgg gagatatccc
     1681 actgttgcag gccggattca aaccactcct gcattttgtt tgccacctgc tcctgcaacg
     1741 caccgctgcg ggtccatgcc tgcagcattt cgctgaaaga gggcagatca aagaagaagt
     1801 gttcagaatc acgcatcact ggtgtagcac cagaaaccac cgatttcggc tcgatcaatt
     1861 cagtcgggct gtaggtcgcg ccgcagactt cgcagttatc gccgtattga tccggggatt
     1921 tacatttcgg gcaggtgcct ttcacaaaac ggtccggcag gaacatgcct ttttccggat
     1981 cgtacagctg agagatggtg cggtttttaa taaaaccgtt ttctttcagg cgagagtaga
     2041 taagttctga caactggcgg ttctcttcgc tgtgcgtcga gtgatagttg tcatagctga
     2101 tgttaaagcc tgcgaaatca gtctgatgct cctgactcat ttcgccaatc atctgctccg
     2161 gggtgatacc gagctgctga gctttcagca tgatcggtgt accgtgggca tcgtcggcgc
     2221 agatgaagtt gacctcgtgg ccgcgcattc gctggtaacg gacccagaca tcagcctgga
     2281 tgtgctccag catatggccg aggtggattg agccgttagc gtacggcagt gcgcacgtca
     2341 ccagaatttt cttcgcgact tgagtcatag taggcattac ttcttaatag tgaaaagggg
     2401 ttagatagta ccaaatggga aaatgttaag taagtacttc tctatattct tcaggtaaac
     2461 tatcagctga tggtttatct atacaaaaaa ggagtcggga tgaacgaaca atcccaggcc
     2521 aaatcaccag aagccttgcg cgcaatggtc gccgggacac tggccaattt tcagcaccca
     2581 accctgaagc ataacctcac cacgctgaaa gcgttgcacc atgtcgcctg gatggacgac
     2641 acgttgcatg tggaactggt tatgccgttt gtctggcata gcgcgttcga agagttaaaa
     2701 gagcaatgta gcgccgaact gctgcgcatc accggggcga aagctatcga ctggaaactg
     2761 tcgcataaca tagccactct gaaacgcgtg aagaatcagc cgggaattaa cggtgtgaag
     2821 aacattatcg cggtgagttc cggtaaaggc ggcgtgggta aatcgtccac ggcggtaaac
     2881 ctggcgctgg cactggcggc ggaaggggcg aaagtcggca ttctggacgc cgatatctac
     2941 ggcccatcaa ttcccaccat gctgggcgca gaaaatcagc gtccaacttc gccggacggt
     3001 acccacatgg caccgattat gtctcatggc ctggcaacca attcaattgg ttatctggtc
     3061 actgacgaca atgcgatggt gtggcgtgga ccgatggcga gcaaggcgtt aatgcagatg
     3121 ttgcaggaaa ccttgtggcc ggatctcgac tatctggtgc ttgatatgcc gccaggcacc
     3181 ggtgacattc aattgacgct ggcgcaaaac attccagtaa ctggtgcagt cgtggtcacc
     3241 accccgcagg acatcgcgtt gatcgatgcg aaaaaaggca ttgtgatgtt cgagaaagtc
     3301 gaggttccgg tgctcggtat tgtcgagaac atgagtgtgc atatttgcag taactgtggt
     3361 caccatgaac cgattttcgg taccggtggt gcagagaaac tggctgaaaa ataccataca
     3421 cagctgctgg ggcagatgcc gttacacatc tccttacgtg aggatctcga taaaggcacg
     3481 ccaacggtta tcagccgtcc ggagagcgaa tttaccgcta tctaccgcca gctggctgac
     3541 cgcgttgcag ctcagctcta ctggcagggt gaagtcattc ctggcgagat ttccttccgc
     3601 gcggtctaac gcctattaac cattctggtt attcttcata catcgcagag atgttatatt
     3661 gatgcggtgt atttgttttt gccgcattaa tgaacactta tttaatactg cgaatattct
     3721 gcggaccttc gtccgaaact ctacaaagtc gagtgatcgt acaggcaatg cttcattatc
     3781 atccttcgtg ctgtgtagtg gatgtacagc tatagtttgt tatgcactca ttctatccag
     3841 gcataacctt tactacaggg ataacgttca atgaataagt attggctgtc aggaattatt
     3901 tttcttgcct atggattggc ttctcccgca gtctcttcag agactgccac attaacgatt
     3961 aatggcagga tcagtccccc aacatgcagt atggctatgg ttaatagtca accacaacag
     4021 cattgtggtc agttaacgta taatgttgac acccgtcatc aggtttcttc gcccgtcaaa
     4081 ggtgttacga cagaagtggt tgttgcaggt agcgatagca aacgtcgaat cgtgttaaat
     4141 cgctacgatt aatttggcaa cctgatttta attgcattta gaaagacgaa atcatttata
     4201 taaatgattt cgtctttttt attgatatca acaataccat ttacataaat ctacattaat
     4261 taatagatgt gataaatatt taatattctt aagtatttgt tgtatttata ttgcattggt
     4321 taatttgttt taaatcaata aaacaatatg tttacattgc ggcagctttt ttgatgtgag
     4381 aaatttgttt ccaagagata cagactctta acaaccaaaa ataaatttta ttaaggaaca
     4441 tattatgaaa cgttcaatta ttgctgccgc tgtcttttct tcttttttta tgagcgctgg
     4501 agtatttgct gcagacgttg ataccggaac attaaccatt aaaggaaata ttgcagaatc
     4561 tccgtgtaaa ttcgaagcgg gtggtgattc agtaagtatt aatatgccga ctgtaccaac
     4621 cactgtcttt gaaggtaaag ctaaatattc tacctatgat gatgcagtcg gtgtaaccag
     4681 cagcatgtta aaaattagct gcccgaaaga agttgctggt gtaaaactct cgttgatcac
     4741 aaacgacaaa ataaccggta acgataaggc gatagccagt agcaacgata ccgtgggtta
     4801 ctatctctat ttaggtgata acagcgatgt cctagatgtt tctgcacctt ttaacattga
     4861 gagttataaa acagcagaag gtcaatatgc tattccgttt aaagcaaaat acctgaaact
     4921 gacagataac tcagtgcaat caggtgatgt gttatcttct ctggttatgc gtgtagcgca
     4981 ggattaataa ataaaattgc aagtttctcc aggtaataaa catggccgct atccccatgg
     5041 cggcttttta atttaagagg cattaagatg aaaggattat tatctttact cattttttct
     5101 atggtccttc ctgcacatgc cggaattgtt atctacggga cgcgcattat ttacccggca
     5161 gaaaataaag aagttatggt gcagttgatg aaccagggaa atcgttcttc gctgctgcag
     5221 gcgtggattg atgatggcga tacgtcgtta ccaccagaaa aaattcaggt tcctttcatg
     5281 ttaacgccac cagtggcaaa aataggggca aattcagggc agcaagtaaa aatcaaaatt
     5341 atgccgaata aactgcccac taataaagaa agcatttttt atctgaatgt tctggatatt
     5401 ccaccaaata gtccagagca agaaggcaag aatgcactga agtttgcgat gcaaaacaga
     5461 attaagttgt tttaccggcc agcgggtatt gctccggtaa ataaagcgac ctttaaaaag
     5521 ttactggtaa atcgcagtgg caatggattg gtgataaaaa atgactcagc taattgggtg
     5581 acgatttcgg atgtcaaagc taataatgtt aaagtcaatt atgaaactat tatgattgcc
     5641 cccttagaaa gtcagagtgt taatatcaaa agtaataatg caaataactg gtatctgacc
     5701 attatcaatg accacggcaa ctatattagt gacaaaattt aattgcagga gctgcctatg
     5761 ttgagaatga ccccacttgc atcagcaatc gtagcgttat tgctcggcat tgaagcttat
     5821 gcagctgaag aaacctttga tacccatttt atgataggtg gaatgaaaga ccagcaggtt
     5881 gcaaatattc gtcttgatga taatcaaccc ttaccggggc agtatgacat cgatatttat
     5941 gtcaataagc aatggcgcgg gaaatatgag attattgtta aagacaaccc gcaagaaaca
     6001 tgtttatcaa gagaagttat caagcggtta ggcattaata gcgataactt cgccagcggt
     6061 aagcaatgtt taacatttga gcaacttgtt cagggtggga gctatagctg ggatatcggg
     6121 gtttttcgtc tcgatttcag tgtcccgcag gcttgggtgg aagaactgga aagtggctat
     6181 gttccaccgg aaaactggga gcggggtatt aatgcgtttt atacttctta ttatgtgagt
     6241 cagtattaca gcgactataa agcgtcgggt aacaacaaga gtacatatgt acgttttaac
     6301 agcgggttaa atttactgga gtggcaactg cattctgatg ccagtttcag taaaacaaat
     6361 aacaatccag gggtgtggaa aagcaatacc ctgtatctgg aacgtggatt tgcccaattt
     6421 ctcggcacgc ttcgcgtggg tgatatgtac acatcaagcg atatttttga ttctgttcgc
     6481 ttcagcggtg tgcggttgtt tcgtgatatg cagatgttgc ctaactcgaa acaaaatttt
     6541 acgccacggg tgcaggggat tgctcagagt aacgcgttgg taactattga acagaatggt
     6601 tttgtggttt atcagaaaga ggttcctcct ggcccgtttg cgattacaga tttgcagttg
     6661 gccggtggtg gagcagatct tgatgttagc gtgaaagagg cggacggctc ggtaaccacc
     6721 tatctggtgc cttatgcagc ggtgccaaat atgctgcaac ccggcgtgtc gaaatatgat
     6781 tttgcggcgg gccgtagcca tattgaaggg gcgagcaagc aaagtgattt tgtccaggca
     6841 ggttatcagt atggttttaa taacttattg acgctgtatg gcggcacgat ggttgctaat
     6901 aattactatg cgtttaccct cggaacgggt tggaacacgc gcattggcgc aatttccgtc
     6961 gatgccacga agtcgcatag taaacaagac aacggcgatg tgtttgacgg gcaaagttat
     7021 caaattgcct acaacaaatt tgtgagccaa acgtcgacgc gttttggtct ggcggcctgg
     7081 cgttattcgt cgcgtgatta ccggacattt aacgatcacg tttgggcaaa caataaagat
     7141 aattatcgcc gtgatgaaaa cgatatctat gacattgccg attattacca gaacgatttt
     7201 ggccgtaaaa atagcttttc tgccaatatg agccagtcat tgccagaagg ctggggttct
     7261 gtgtccttaa gtacgttatg gcgagattac tgggggcgta gcggcagcag taaggattat
     7321 cagttgagtt attccaataa ctggcggcgg ataagctata ccctcgcggc aagccaggcg
     7381 tatggcgaga atcatcatga agagaaacgt tttaatattt ttatatcaat tccctgtgat
     7441 tggggtgatg acgttacgac gcctcgtcgg caaatatata tgtctaactc aacgacgttt
     7501 gatgatcagg gttttgcctc aaataatacg ggattatcag gaacagtagg gagtcgggat
     7561 cagttcaatt atggtgtcaa cctgagtcat caacatcagg gaaatgaaac gacagctggg
     7621 gcgaatttga cctggaacgc gccggttgcg acagtgaatg gcagttatag tcagtcgagt
     7681 acttatcgac agactggagc cagtgtttca gggggcattg tcgcctggtc gggtggcgtt
     7741 aatctggcga accgtctttc cgaaacgttt gctgtgatga atgcgccagg aattaaagat
     7801 gcttatgtca atgggcaaaa atatcgcaca acaaaccgta atggagtggt ggtatacgac
     7861 ggaatgacac cttatcggga aaatcacctg atgctggatg tgtcgcaaag cgatagcgaa
     7921 gcagaattac gtggcaaccg gaaaattgcc gccccttatc gcggcgcggt tgtactggtt
     7981 aattttgata ccgatcagcg caagccctgg tttataaaag cgttaagagc ggatggtcaa
     8041 ccattaacgt ttggttatga agtcaatgat atccatggtc ataatattgg cgttgtcggc
     8101 cagggaagcc agttatttat tcgcaccaat gaaataccgc catcggttaa tgtagcaatt
     8161 gataagcaac aaggactttc atgcacaatc accttcggta aagagattga tgaaagtaga
     8221 aattatattt gccagtaata gtcaggtggt tctatggaaa ttcgcataat gctatttata
     8281 ttaatgatga tggttatgcc tgtgagctat gcggcatgtt atagtgagtt atctgttcag
     8341 cacaatttgg ttgttcaggg gaattttgca cttactcaaa cacaaatagc gacatatgag
     8401 cataatttta atgattcgtc atgcgtgagt acaaatacta tcacccctat gagcccatcg
     8461 gatattattg ttggacttta taacgatacc attaaattaa atttacattt tgaatggacc
     8521 aataaaaaca acatcacgtt gtcaaataat cagagcagtt tcaccagtgg ttattcggta
     8581 acggtgacac ctgcggccag taatgcaaaa gtgaatattt ctgcgggggg cggtggttca
     8641 gtgatgatta atggtgttgc gacattatcc agtgcttcat catcgacacg cgggagtgcc
     8701 gcagtacaat ttctactgtg tttattaggt ggcaagtcat gggatgcatg tgtaaatagc
     8761 tacagaaatg cattggcaca aaatgcaggt gtctattcct tcaatctgac attgtcatac
     8821 aacccgataa ccacaacctg caaacaggac gatttattaa ttactttaga cagtattccc
     8881 gtttcacaat taccagccac aggtaacaaa gcaacaataa atagtaaaaa aagggatatt
     8941 attctgcgtt gtaaaaattt attaggtcaa caaaatcaaa catcacggaa aatgcaggtg
     9001 tatttatcaa gttctgactt gttaaccaac agcgacacaa tactgaaagg tgcggaagat
     9061 aatgcgtagg atttattctt gaaagtaatg gttcgccagt cacactttta aatatcacta
     9121 acagcagtaa aggatacaca aatttaaaag aaattgcggc aaagtcaaaa cttacagata
     9181 caacggtttc aattccgata acagccagtt actacgtcta cgatacaaac aaagttaaat
     9241 ctggcgcact ggaggcaacc gcattaatca acgtgaaata cgactaataa caagcaagac
     9301 gagcaagtgg ctaataataa aaaataacaa ggtgtctgca tttccccctc atgatgaggg
     9361 ggcttttttt agcgatgata aaaaatctca ccgtcgtagg ctttaatgat tttaccgtcg
     9421 gtgtcgctga tcaacacgta cgcgccaccc atataggtcc agtgcgtccc ggcatcaggc
     9481 gcgggcaggt tacgtaactg ccactgctta atgttgtact catcggtgcg gtacatctct
     9541 gggacggtat cacctggttt gaaatgctta aagtcagcaa tgaaactact taactcatac
     9601 ttagaaattc ccgaggtatt ggtcgaaccc gcggtggctg gtgcggccca ggcggcactg
     9661 gtaaccagca aaagcacacc cagcaacatc ttatttttaa tggtcataaa ctctccactt
     9721 tggggatctg taatatcagt cgcatatctt tcctgccata caatataact gacaagcgat
     9781 attttgttag tgatcccatg atttcgtaaa aagtgtaaat gaaaggatgc cctaaaagca
     9841 tccttagcga gcagagagtc agcggaaata gctgatttaa gaatcatcct gggctgaaga
     9901 taacatgcaa gcctaaagga ttgagttatc gcattatgcc catgaagcca tgtacgccca
     9961 tatacacacc gactaagcca atcaacagac tggaaaaata gggggcgcgt ttagcgagag
    10021 tgttaaatcc gctccagcgt tttgcgacct gctgaacgct gattgctgcg ccaacgccta
    10081 cggtgacaag cgttaacgcc aggccaatgc tgaaactgac gaccagtgtt gcgcccagtg
    10141 tcagggcttt caactgaatg catatcaaca gcacggtaat tgctgccggg caggggataa
    10201 ggccaccggt taagccaaat aacaaaattt gccagttgat gacctctcta ccatcaaagc
    10261 gtcgtttaat gtcattggca tgggctcgtg catgggcatc ctgatactcg ccatgttcgt
    10321 gatggtgatg atgtccttgg tcgtggtgat gttcgtggtc ttgatgatgt tcgtgatgat
    10381 ggtgatgatg ctcataatca tgcccgtgca tattctccag ccagttgcgt tcgccgcgcc
    10441 aggtacgcca gaacatccag aacacggtgc caataatgat cactgcggaa atcagctgga
    10501 gccacggttc tgctgattga gcagtaaagc gcttactgat caccatcccg ccaaaggcaa
    10561 tcaaccagac caccgcggta tgcgaaatgg ttgctgccag tccgagcatc accgcctgtt
    10621 taatggtgcc tttgatggcg atgataaacg ccgccatcat cgtttttgag tgccctggtt
    10681 ccaggccatg aagcgcacca agtaagatgg cgctggggat gaagaaccag gcgtttcctt
    10741 gctgaagaag agttgtaaat tcggtcatga taataattct tagtattaat tcggcaatct
    10801 gattctactc ccccccagta cctgatacta ccccccagta gattaatagt gctatgattt
    10861 ttcatattct tgttaaccag gtgttgccat gtctcataca atccgtgata aacagaaact
    10921 gaaagcgcgt gccagtaaga ttcagggcca ggtcgtggcg ctcaagaaaa tgctcgacga
    10981 gccgcacgaa tgcgctgcag ttttacaaca gattgctgct atccgtggag cggtaaacgg
    11041 tctgatgcgg gaagtgatta aaggtcatct gacggaacac atcgttcacc agggggatga
    11101 gctaaaacgt gaagaagatc tggatgtcgt tctgaaggtg ctggattcat atatcaaata
    11161 atttattaac gcgattgtaa aactgccgtt tttcctcgtt tacaacgggt gcgctggaca
    11221 ttaccatcct cctctgcgat ttatcatcgc aaccaaacga ctcggggtgc ccttctgcgt
    11281 gaaggctgag aaatacccgt atcacctgat ctggataatg ccagcgtagg gaagtcacgg
    11341 accaccaggt cattgcttct tcacgttatg gcaggagcaa actatgcaag tcgacctgct
    11401 gagttcagcg caatctgcgc acgcgttaca cctttttcac caacattccc ctcttgtgca
    11461 ctgcatgacc aatgatgtgg tgcaaacctt taccgccaat accttgctgg cgctcggtgc
    11521 atcgccagcg atggttatcg aaaccgaaga ggccagtcag tttgcggcta tcgccagtgc
    11581 cttgttgatt aacgttggca cactgacgca gccacgcgca caggcgatgc gtgctgccgt
    11641 tgagcaagca aaaagctctc aaacacgctg gacgcttgat ccagtagcgg ttggtgcgct
    11701 cgattatcgc cgccattttt gtcatgaact tttatctttt aaaccggcag cgatacgtgg
    11761 taatgcttcg gaaatcatgg cattagctgg cattgctaat ggcggacggg gagtggatac
    11821 cactgacgcc gcagctaacg cgatacccgc tgcacaaaca ctggcacggg aaactggcgc
    11881 aatcgtcgtg gtcactggcg aggtggatta tgttaccgat ggacatcgta tcattggtat
    11941 tcacggtggc gatccgttaa tgaccaaagt ggtaggaact ggctgtgcat tatcggcggt
    12001 tgtcgctgcc tgctgtgcgt taccaggcga tacgctggaa aatgtcgcat ctgcctgtca
    12061 ctggatgaaa caagccggag aacgcgcagt cgccagaagc gaggggccag gcagttttgt
    12121 tccacatttc cttgatgcgc tctggcaatt gacgcaggag gtgcaggcat gaaacgaatt
    12181 aacgctctga cgattgccgg tactgatccg agtggtggtg cggggattca ggccgatctt
    12241 aaaaccttct cggcacttgg cgcttatggt tgctcagtta ttactgcact ggtggcgcaa
    12301 aatacccgtg gcgtacagtc ggtgtatcgc attgagcctg attttgtcgc cgcccagctc
    12361 gattcggtgt tcagcgatgt gcgaatcgat accactaaaa tcggtatgtt ggcggaaacc
    12421 gatattgttg aagcggtggc agaacggttg caacgttatc agatccaaaa cgtggtactc
    12481 gacaccgtta tgctggcaaa aagcggcgac ccgctgcttt caccttcggc ggttgctacg
    12541 ctgcgcagtc gattattgcc acaggtttca ttaataacgc caaacttgcc cgaagctgcc
    12601 gccttgctcg acgcgccaca cgcgcgcacc gaacaggaaa tgctggaaca agggcgatcg
    12661 ctgttggcga tgggctgtgg cgcagtgcta atgaaaggtg gtcatctgga tgatgagcaa
    12721 agcccggact ggctgtttac ccgcgagggt gaacaacggt ttaccgcacc gcgcattatg
    12781 accaaaaaca cccacggcac tggttgtaca ctctctgcgg cgttggctgc actacgcccg
    12841 cgccatacaa actgggctga caccgtacag gaggcaaaaa gctggctttc atcggcgtta
    12901 gcccaggccg acacgctgga agttggtcac ggtattggtc cggttcacca cttccacgcc
    12961 tggtggtgag caccgtacta tagctgcttt ggttattgtc agcctttggc actgacattc
    13021 agggatgaaa ccaatgcagc taagaattac cagtcgaaaa aaactcaccg ctctattatg
    13081 cgctcttggg ctaatttcca tcgttgcaat ttatccgcgt cagacggtga attttttcta
    13141 ttcgacagca gttcagatta cagactacat ccacttctac ggttatcgcc cggttaaatc
    13201 tttcgctatt cgcattcctg ccagttacac cattcacgga ttagatgttt cgcgctggca
    13261 ggagcgtatc gactggcagc gggtggcaaa aatgcgcgac aacgatatcc gcttacagtt
    13321 tgcttttatt aaggctacgg aaggcgaaaa gctggtggac ccctattttt cgcgtaactg
    13381 gcaattaagc cgcgaaaatg gcctgctgcg cggggcgtat cattatttct ccccgtcggt
    13441 atccgcttca gttcaggcgc gattatttct gcaaacggtg gatttctcac aaggcgattt
    13501 ccctgccgtg ctggacgtag aagaacgggg aaaattatcg gcaaaagaat tacgcaagcg
    13561 ggtaagtcag tggctaaaaa tggtcgaaaa acgcacggga aaaaagccga ttatttactc
    13621 aggagccgtt ttttatcaca ccaatctggc gggctatttc aatgaatatc cgtggtgggt
    13681 ggctcactat tatcaacgtc gtccggacaa tgacggcatg gcctggcgct tctggcagca
    13741 ttccgaccgt ggacaggtag atggcatcaa tggtccggtg gattttaatg tgtttaatgg
    13801 cacggtggat gagttgcagg cattcgttga tgggattaaa gaaacgcctt aaataagaag
    13861 cagtgatata ctcccaccag actccctggc aggaaaaaga caaatggaac aagcgcatac
    13921 ccagctgatt gcccaactga acgagcgtat tttagcggcg gataacacgc cgctttatat
    13981 taagtttgcc gaaacggtaa aaaatgccgt gcgcagcggg gtgctggagc atggcaatat
    14041 tttgcccggt gagcgtgatt taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa
    14101 ggcgatgcag gcgctggaag aagagggcgt ggtgacgcgt tcgcgcggtt acggcacgca
    14161 gatcaacaac atcttcgaat actcgttgaa agaagcgaga ggtttttctc agcaggtggt
    14221 attgcgcggg aaaaagcccg atacgctatg ggttaataag cgtgtcgtga aatgtcccga
    14281 agaagttgcg cagcagctgg cggtcgaagc gggaagtgat gtctttttgc ttaagcgtat
    14341 tcgctatgtc gatgaagaag cggtatcgat tgaggaatcg tgggttccgg cgcatttaat
    14401 tcatgatgtt gatgccatcg gaatttctct ttatgactat ttccgcagcc agcacattta
    14461 cccacaacgt acgcgttccc gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca
    14521 tattcagtta gatagcaaaa taccggtgct ggtgatcaag caagtggcgc ttgaccaaca
    14581 gcaacggcct attgagtaca gcattagcca ctgtcgcagc gatctatacg tttttgtgtg
    14641 cgaggagtag ttcctcgcgc gttggcgcac aatcaccgcc ccgatgcccg acaacccacg
    14701 acgccactgc attgcccagt aatacggcat ccgccagtgg cagaccagag gccagcccgg
    14761 caagtacgcc acctgcatga ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa
    14821 atgcccgaat gcagccagaa gcgtcgttgc tgaaatacca tgcgccttct ttatcgagac
    14881 gaacgatcaa cggcgcggca aatttctcct gccattgctt gccaagtgtt gttatctctg
    14941 cagataaagc aaaacgttcg gcggcaatct cagcctcttg acgattgagt gacactaaag
    15001 gtcgacaggc catgatccgc gccagtaatg cgtccgggat atcgccaata cgtgggccaa
    15061 aatcgataaa cggcgtcacg tcttgcagct tttccagcca ttccactaac aattcgccgc
    15121 agggcgaggc cagttgataa ccggaaaaat agagcaggtt gccaggcgca acggttaatc
    15181 gtgccaacca ttggcgattc cattgatttt ccacaccgct gaatgacata aaagtgcgtt
    15241 cgccatccgg ctcaaccagt gccagacacc agccgttatc gctttcggcg ttatcgatca
    15301 ggctgattaa cccctctttc gccatccggt tgcgaataat ctccgcccac acgccctgac
    15361 cgagcggcaa ggcattaccc gcttcgatgc cgaggcgctt taacgccacg gcaatattca
    15421 gtgcgcagcc gccaacgtta acgctctgct gtttcagttc gatatcgcac ccacgccagg
    15481 ggagggcata agcgtcggcg atcacgtcaa tgaccgccgc gccgacgacc atcaccggct
    15541 gacgcgtggt taattcaggc agcagtgtgt gtaatcgagc gccgctcata ccgcctcccg
    15601 ttgttgacga tatttctcca gcgctgtggc atagcggttg aaatcaagct gatttaccgc
    15661 atccagctcc gcctttaacg caggatcgac agcgttaatg ccatgcaacg cgccgcaaat
    15721 cgccgtcgcc atagcaccta tggtgtctgt gtcgccgcca agattggcgc acaggacggc
    15781 gcagcggttt gggtcggttt gtgccagttc aactagcgca atggcgcacg gaacggactc
    15841 aatagtgctg gtgcctgcgc caacgacctg gtaaagctgt tcgctggcgg attcggtgcc
    15901 gtcggcatta cgcacaattt tcagcgcaat ctccagacgt gccgccagtg aggcgctgaa
    15961 cgtggtggtg cgtttttgct gtgcatgtcg cgcaattgaa ggcaatgaat caacaatcgc
    16021 tgaccagcgt tctccgtcaa tggcacgaga aatcgcccat gcgatgacta ccgcgcctgc
    16081 aaccgccaga tcggatttat gggtcgggct ggacgccagc gccacatcat caataaagga
    16141 atcaacatca cgcgctggaa gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt
    16201 ggttacgccg ttgttttcca gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc
    16261 aatcttcgag gtcggaccta gtacgttttt gttaaaggcg tcgaaacgca acgcccagtc
    16321 gagaatatta cgcccaatca gattcggatc gatcttgcct tcacattcca gtaacgcatc
    16381 cgccagacac aggccatcga ggtatcgtcg gtgaattcgg cgcggttaaa ataacaggcc
    16441 gcgttattct cctttggtcc aggcagaaaa cggtcaatcc agccaaagtg tgctttaacg
    16501 cggctgcgtg gccaaagctc ggagggcatc cccatcgcat cccctaacgc ctgcccataa
    16561 agagcaccga gaatacgttc tgttttcatt taacttcccc ttgtgtcaac gcaatatctc
    16621 gatcatcgac cttgatagcc gtaatttcgt tgtcggattc gcgggaaaaa atcatgaaca
    16681 gcacggcgat aatggcaatc atcaccgcgc cgaaagtcca catcccggac cagttgaaag
    16741 tcagtccgtt taccggttcc tgataagcga acatcttttc cgtcatcaca ccgccaagac
    16801 gatagccgag caaactgccg aagccctggc agcagagcgt gatcagcccc tgcgcagcgg
    16861 tacgcatatg cacgggggct tttttatcga catagatgta agcggtaacg tagtaaaaat
    16921 cgtaacttac gccgtgaagc aaaataccga ggaacagtaa cgcgtaggtg aaattgacag
    16981 gtaatgtaag gtacgcgatg acaataccac ataataatac cttttgaata cctttgcggt
    17041 aaaaacttga ccgcgctaac attttttctg atgaatcgag ccaacagaaa acgctgaaaa
    17101 aacatccaaa agatggaaaa actcgcccgt aacgaccagg attatgattg cccgcaaaag
    17161 gcgggccagg acaatccata gccgatatcc aatcggaatt tacgggagca tagtaatgac
    17221 agatattgcg cagttgcttg gcaaagacgc cgacaacctt ttacagcacc gttgtatgac
    17281 tattccttct gaccagcttt atctccccgg acatgactac gtagaccgcg tgatgattga
    17341 caataatcgc ccgccagcgg tgttacgtaa tatgcagacg ttgtacaaca ccgggcgtct
    17401 ggctggcaca ggatatcttt ctattctgcc ggttgaccag ggcgttgagc actctgccgg
    17461 agcttcattt gctgctaacc cgctctactt tgacccgaaa aacattgttg aactggcgat
    17521 cgaagcgggc tgtaactgtg tggcatcaac ttacggcgtg ttggtgtcgg tatcgcggcg
    17581 ctatgcgcat cgcattccat ttctcgtcaa acttaatcac aacgagacgc taagttaccc
    17641 gaatacctac gatcaaacgc tgtatgccag cgtggagcag gccttcaaca tgggcgcggt
    17701 ggcggttggt gcgactatct attttggctc ggaagagtca cgtcgccaga ttgaagaaat
    17761 ttctgcggcc tttgaacgcg cgcacgagct gggcatggtg gcagtgctgt gggcctattt
    17821 gcgaaactcc gcctttaaga aagatggcgt tgattaccat gtttccgccg acctgactgg
    17881 tcaggcgaac catctggcgg caaccatagg tgcagatatc gtcaaacaaa aaatggcgga
    17941 aaataacggc ggctataaag cgattaatta cggttatacc gacgatcgcg tgtacagcaa
    18001 gttaaccagc gaaaacccga ttgatctggt gcgttatcag ttagctaact gctatatggg
    18061 ccgggctggg ttgataaact ccggcggtgc tgcaggcggt gaaactgacc tcagcgatgc
    18121 agtgcgtact gcggttatca acaaacgcgc aggcggaatg gggctgattc ttggacgtaa
    18181 ggcgttcaag aaatcgatgg ctgacggcgt gaaactgatt aatgccgtgc aggacgttta
    18241 tctcgatagc aaaattacta ttgcctgata cgcctcatac gaacagcacc accccgtagg
    18301 acggataagg cgtttacact gcatccggca acctatgcct gatgcgacgc ggaagcgtct
    18361 tatcatgcct acatagcact gccacgtatg tttacaccgc atccggcata aaaacacgcg
    18421 cactttgcta cggcttccct atcgggaggc cgtttttttg cctttcactc ctcgaacaat
    18481 tttcatattg tcgtttttgt gatcgttatc tcgacattta aaagcaaata atttcattat
    18541 tttttgaaat cgaaagcaaa caacaagata tgaaaatgta cgtggtatcg acaaagcaga
    18601 tgctgaacaa cgcacagcgc ggcggttatg cggttccggc attcaatatt cacaatctcg
    18661 aaacgatgca agtggtggtg gaaaccgctg ccaacctgca tgcgccggtc atcatcgccg
    18721 gaacgcctgg cacatttact catgctggta cagaaaatct gttggcgctg gtcagcgcga
    18781 tggcgaagca atatcaccat ccactggcaa ttcatctcga ccatcacacg aaatttgacg
    18841 atatcgctca gaaggttcgt tctggcgtgc gctcagtcat gattgacgcc tcgcatttgc
    18901 cttttgcgca aaatatttca cgggtcaaag aggtggtgga tttttgccat cgctttgatg
    18961 tcagcgtcga ggcggagctg gggcaacttg gcggccagga agatgatgtg caagtcaatg
    19021 aagccgatgc gttgtacacc aaccccgcac aggcgcgtga atttgccgag gcaaccggga
    19081 ttgattccct ggcggtcgcc atcggcacgg ctcacggtat gtatgccagc gcaccggtgc
    19141 ttgatttttc tcgactggag aacattcgcc agtgggtgaa cttaccgctg gtgctgcatg
    19201 gcgcgtcagg attatcgact aaggatattc agcaaaccat caaactgggg atatgcaaaa
    19261 tcaacgttgc aacggagctg aaaaatgcct tctcgcagtc gttaaaaaat tacctgaccg
    19321 agcaccccga agcgaccgat ccccgggatt atttgcagtc ggctaaatcc gcaatgcgcg
    19381 atgtggtgag caaagtgatt gccgattgtg gctgcgaggg cagggcataa cgcacctgcc
    19441 atttaacaag gaaaaaacat gaaaacgtta attgcccggc ataaagctgg tgaacatatc
    19501 ggcatatgtt cagtctgttc tgcccatccg ttggttatcg aagcggcgct ggcatttgat
    19561 cgcaacagca cgcgcaaagt gctgattgaa gcaacgtcaa accaggtcaa tcaatttggc
    19621 ggttataccg gaatgacact ggcagacttt cgcgaatttg tttttacgat tgccgataaa
    19681 gttgggtttg cacgcgaacg cattattctc ggcggcgatc atctggggcc aaactgctgg
    19741 cagcaagaaa atgcggatgc ggcgatggaa aaatccgtcg agctggtaaa agcatatgtt
    19801 cgtgctggct tcagtaaaat tcatcttgat gcgtcaatgt cctgcgcggg ggatcccata
    19861 ccgttagcac cagaaacggt tgcggaacga gctgctgtgc tttgctttgc tttgctgcgg
    19921 aaagtgtggc gacagattgc cagcgtgagc aactgagcta tgtcattggc actgaagttc
    19981 cggttccggg cggtgaggcc agcgccattc agtcagtaca catcacccat gttgaagatg
    20041 ccgccaatac tttacgtacg catcaaaagg cctttattgc ccgtgggctg acagaggcgt
    20101 taacacgtgt gattgccatc gtggtgcagc cgggtgtgga atttgatcac agcaatatta
    20161 tccattatca gccgcaggaa gcgcaggcgc tggcgcaatg gatagaaaac acccgaatgg
    20221 tttatgaagc acattctacc gattaccaga cccggacggc ttattgggaa ttagtccgcg
    20281 atcactttgc aatattgaaa gtcggtcctg cattaacctt tgctttacgc gaggcgatat
    20341 ttgcactggc acaaattgag caggaactta tcgcccccga aaatcgcagc ggttgcctgg
    20401 cggtaattga agaagtgatg ctcgacgaac cgcaatactg gaaaaaatat tatcgcacgg
    20461 gttttaacga ttcattactg gatattcgtt acagcctgtc ggatcgtatt cgttattact
    20521 ggccgcatag tcggattaaa aatagcgttg aaacgatgat ggtgaatctg caaggcgtgg
    20581 acatcccact gggcatgatt agtcagtatc ttcccaaaca atttgaacgc attcagtccg
    20641 gggaattatc agcaatgccg catcagctta ttatgaataa aatttatgat gttttgcgcg
    20701 cctatcgcta cggttgtgcg gaataaggac ggtatatgac taacctgttt gttcgtagcg
    20761 gaatttcttt tgtcgatcgt agcgaagttt taacctatat cggtaatgag atgctcgcga
    20821 aaggtgtggt tcatgatacc tggccacagg cattaattgc cagagaagca gaattcccta
    20881 ccgggataat gcttgagcag cacgccattg caataccgca ttgtgaggcg attcatgcta
    20941 agtcgtcagc catttatctg ttaaggccaa caaataaagt tcattttcag caagcggatg
    21001 atgataacga cgtggcggta tcgttggtta ttgcgttgat tgtggaaaat ccgcagcagc
    21061 aattgaaact tttacgctgt ttatttggca agttacaaca gcccgatatc gtcgagacac
    21121 taattactct tcctgaaact cagttaaagg aatacttcac aaagtatgtt ttagattcag
    21181 acgaataaat ccctctgtaa caataaaaag gactatttat gaaacgcaag attattgtcg
    21241 cttgcggagg cgcggttgcg acctctacga tggcggcgga agaaattaaa gagttgtgtc
    21301 agagtcataa tattcctgtt gaattaatcc agtgtcgggt taatgaaata gaaacctata
    21361 tggatggtgt gcatttgata tgtaccactg ccagggtgga tcgtagtttt ggcgatattc
    21421 cgttagttca cggcatgcct tttgtttctg gtgtcggtat cgaagcatta caaaataaaa
    21481 ttctgactat cttacagggg tgacctatgt tttcagaagt catgcgttat attctcgacc
    21541 tcggccctac agtgatgctg ccggttgtca tcattatttt ttctaaaata ttaggcatga
    21601 aggcaggcga ttgctttaaa gcgggtctgc atatcgggat tggctttgtt ggcattggcc
    21661 ttgtgattgg cttaatgctg gattccattg gtccggcggc gaaagcgatg gcggaaaatt
    21721 tcgacctgaa tctgcatgtg gtcgatgtcg gctggccggg ctcttcacca atgacctgga
    21781 cgtcgcaaat tgcgctggtg gcgattccga ttgcgatatt ggttaacgtg gcgatgttac
    21841 tgacccgtat gacgcgggtg gtaaatgttg atatctggaa tatctggcat atgaccttca
    21901 ccggcagcgt tgctgcacct ggcaaccggt tcatggatga tagggatggc gggtgtggta
    21961 attcacgcgg cgtttgttta taagctcggc gactggtttg cccgcgatac ccgaaatttc
    22021 tttgagctgg aaggtattgc tattccgcac ggtacgtcgg cgtatatggg gccgattgcg
    22081 gtgctggtcg atgctatcat cgagaaaatc ccaggcgtta accgaattaa atttagcgcc
    22141 gacgatattc agcgcaaatt tggccctttt ggcgaacctg tcaccgtggg ttttgtgatg
    22201 gggctgatta tcggcatcct cgcgggttac gatgtcaaag gtgtattgca gctggcggta
    22261 aaaacggcgg cagttatgct gctgatgcca cggatgatta aacccatcat ggatggttta
    22321 acgcccatcg ctaagcaggc ccgtagtcgt ttacaggcga agttcggcgg tcaggagttc
    22381 ctgattggcc tggatcccgc attactgctg gggcatacgg cggtggtatc ggcaagcctg
    22441 atttttattc cgctcaccat tttaattgct gtttgtgttc cgggtaatca ggtactgccg
    22501 tttggcgatc ttgccactat cggcttcttc gtggcgatgg cggtcgcggt gcatcgtgga
    22561 aatctgttcc gcaccttaat ctcgggtgtc atcattatga gcatcaccct gtggatcgcg
    22621 acgcaaacta ttggtttgca cacccaactg gcggctaatg ctggggcgtt aaaagccggg
    22681 ggtatggtgg cttcaatgga tcagggcggt tctcccatta cctggttact gattcaggtt
    22741 ttctccccgc aaaatattcc cggtttcatt attatcggcg caatt
//
LOCUS       DADMGC010000061        22597 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.061, whole genome shotgun sequence.
ACCESSION   DADMGC010000061 DADMGC010000000
VERSION     DADMGC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.061"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..610
                     /locus_tag="JNZ73_002279"
     CDS             <1..610
                     /locus_tag="JNZ73_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8732544.1"
                     /translation="PGLKAVGSYRSVLELLNIPHAKLIMDCVILLSVTSCLNSALYTA
                     SRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDS
                     SGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIGFITFVLVVMLFR
                     PAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR"
     gene            631..1289
                     /gene="csiR"
                     /locus_tag="JNZ73_002280"
                     /pseudo
     CDS             631..1289
                     /gene="csiR"
                     /locus_tag="JNZ73_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
     gene            complement(1290..1739)
                     /gene="kbp"
                     /locus_tag="JNZ73_002281"
     CDS             complement(1290..1739)
                     /gene="kbp"
                     /locus_tag="JNZ73_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HAY8732545.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(1823..1981)
                     /locus_tag="JNZ73_002282"
     CDS             complement(1823..1981)
                     /locus_tag="JNZ73_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HAY8732546.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            2164..2463
                     /locus_tag="JNZ73_002283"
     CDS             2164..2463
                     /locus_tag="JNZ73_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8732547.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            2473..2997
                     /gene="ygaP"
                     /locus_tag="JNZ73_002284"
     CDS             2473..2997
                     /gene="ygaP"
                     /locus_tag="JNZ73_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HAY8732548.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKIPWNQRA"
     gene            complement(3044..3448)
                     /gene="stpA"
                     /locus_tag="JNZ73_002285"
     CDS             complement(3044..3448)
                     /gene="stpA"
                     /locus_tag="JNZ73_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HAY8732549.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            4116..4553
                     /gene="alaE"
                     /locus_tag="JNZ73_002286"
     CDS             4116..4553
                     /gene="alaE"
                     /locus_tag="JNZ73_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HAY8732550.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKGWIKNLADILAYVTFQSPVYVAILLVVGAD
                     WHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(4590..4940)
                     /locus_tag="JNZ73_002287"
     CDS             complement(4590..4940)
                     /locus_tag="JNZ73_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HAY8732551.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERQRHEHYGIPHGFSSRV
                     ALERYLNGLFGEAS"
     gene            5092..5421
                     /locus_tag="JNZ73_002288"
     CDS             5092..5421
                     /locus_tag="JNZ73_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY8732552.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     QLSMRKS"
     gene            5669..5914
                     /gene="nrdH"
                     /locus_tag="JNZ73_002289"
     CDS             5669..5914
                     /gene="nrdH"
                     /locus_tag="JNZ73_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAY8732553.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            5911..6321
                     /gene="nrdI"
                     /locus_tag="JNZ73_002290"
     CDS             5911..6321
                     /gene="nrdI"
                     /locus_tag="JNZ73_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAY8732554.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            6294..8438
                     /gene="nrdE"
                     /locus_tag="JNZ73_002291"
     CDS             6294..8438
                     /gene="nrdE"
                     /locus_tag="JNZ73_002291"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAY8732555.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            8448..9407
                     /gene="nrdF"
                     /locus_tag="JNZ73_002292"
     CDS             8448..9407
                     /gene="nrdF"
                     /locus_tag="JNZ73_002292"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAY8732556.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            9762..10964
                     /locus_tag="JNZ73_002293"
     CDS             9762..10964
                     /locus_tag="JNZ73_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HAY8732557.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            10957..12021
                     /gene="proW"
                     /locus_tag="JNZ73_002294"
     CDS             10957..12021
                     /gene="proW"
                     /locus_tag="JNZ73_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HAY8732558.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGACSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            12079..13071
                     /gene="proX"
                     /locus_tag="JNZ73_002295"
     CDS             12079..13071
                     /gene="proX"
                     /locus_tag="JNZ73_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HAY8732559.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            13263..14442
                     /locus_tag="JNZ73_002296"
                     /pseudo
     CDS             13263..14442
                     /locus_tag="JNZ73_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            14566..15303
                     /locus_tag="JNZ73_002297"
     CDS             14566..15303
                     /locus_tag="JNZ73_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAY8732560.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            15293..15628
                     /gene="ygaH"
                     /locus_tag="JNZ73_002298"
     CDS             15293..15628
                     /gene="ygaH"
                     /locus_tag="JNZ73_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HAY8732561.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(15647..15748)
                     /locus_tag="JNZ73_002299"
     CDS             complement(15647..15748)
                     /locus_tag="JNZ73_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732562.1"
                     /translation="MFNGRKRTIHLGMTSLISADIVTTVTNKVNVLF"
     gene            15719..16249
                     /gene="emrR"
                     /locus_tag="JNZ73_002300"
     CDS             15719..16249
                     /gene="emrR"
                     /locus_tag="JNZ73_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HAY8732563.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            16376..17548
                     /gene="emrA"
                     /locus_tag="JNZ73_002301"
                     /pseudo
     CDS             16376..17548
                     /gene="emrA"
                     /locus_tag="JNZ73_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            17565..19103
                     /gene="emrB"
                     /locus_tag="JNZ73_002302"
     CDS             17565..19103
                     /gene="emrB"
                     /locus_tag="JNZ73_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HAY8732564.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(19167..19682)
                     /gene="luxS"
                     /locus_tag="JNZ73_002303"
     CDS             complement(19167..19682)
                     /gene="luxS"
                     /locus_tag="JNZ73_002303"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAY8732565.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(19832..21388)
                     /gene="gshA"
                     /locus_tag="JNZ73_002304"
     CDS             complement(19832..21388)
                     /gene="gshA"
                     /locus_tag="JNZ73_002304"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY8732566.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFHDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(21461..21889)
                     /locus_tag="JNZ73_002305"
                     /pseudo
     CDS             complement(21461..21889)
                     /locus_tag="JNZ73_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
     gene            complement(21886..22452)
                     /gene="yqaB"
                     /locus_tag="JNZ73_002306"
     CDS             complement(21886..22452)
                     /gene="yqaB"
                     /locus_tag="JNZ73_002306"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HAY8732567.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         5436 a         5758 c         5664 g         5739 t
ORIGIN      
        1 gccggggctg aaagccgttg gatcttatcg ctcggttctg gaattgctca atattcccca
       61 tgcgaaatta atcatggact gtgtgatatt actttccgta accagttgcc tgaactcggc
      121 cctgtatacc gcctcaagga tgctctactc cttaagccgt cgcggtgatg ctcccgcggt
      181 aatgggtaaa atcaaccgca gtaaaacccc gtacgtggcg gtgttactct ccaccggcgc
      241 agcattctta acggtggtgg tgaactatta cgcgcctgcg aaggtattta aatttctgat
      301 cgacagctcc ggcgctatcg ccctgctggt ttatttagtc atcgccgttt cacagttgcg
      361 gatgcgcaaa attctgcgag cagaaggaag cgaaattcgc ttgcgaatgt ggctttatcc
      421 ctggctcacc tggctggtca tcggctttat tacctttgtg ttggtagtga tgctattccg
      481 tccggcgcaa cagttagaag tgatctccac cggcttatta gcgataggga ttatctgtac
      541 cgtgccaatt atggctcgct ggaaaaagct ggtattgtgg caaaaaacac ccgttcataa
      601 tacgcgctga taatgatcag gagtcacacc atgaccatta cgtctctgga tggctatcgc
      661 tggctgaaga acgatattat tcgcggtaat tttcaaccgg atgaaaaatt acggatgagt
      721 ttactgacat cgcgttatgc acttggcgtt gggccgttgc gggaagcact ttctcaactg
      781 gtggcggaac ggctggtcac ggtggtgaat caaaaagggt atcgggtggc gtctatgtca
      841 gagcaggagc tgctctgaca tagacgccac ccgatatgga agcgatgctg gtcagtctgg
      901 cgattgcccg cggtggcgat gaatgggagg cagacgttct cgcaaaagcg catctgctca
      961 ataagctgga agcctgtgac gccagcgaga aaatgcttga tgagtgggat ctgcgtcatc
     1021 aggcgtttca tacagcaatt gtggcgggct gtggttctca ctatttgctg caaatgcgtg
     1081 aacggttgtt tgatctggcg gcgcgttatc gatttatctg gctgcggcga acggtgcttt
     1141 cggtggaaat gctggaggat aaacacgatc agcaccagac cctgactgcg gcggtactgg
     1201 cgcgagatac agcgcgcgtc agtgagttaa tgcgccagca tttactgacg ccaattccca
     1261 ttatccagca ggcgatggct ggcaattaac tactcttccg gaatacgcaa cacttgcccc
     1321 ggataaattt tatccgggct ttttagcatc ggtttattcg cttcgaagat tttattgtac
     1381 agattagcgt taccgtagac ctgtttggaa atggcactca gagtgtcgcc agacttaacg
     1441 gtataaaact ggctggcagt ggctggtgtc gccgttttca cctgatcatc aacactggca
     1501 ataccggaaa tattccccac cgcaacaagg attttctcct tcgcctcctg actcaggccg
     1561 tcaccagtga ccgtcgcttt gccgtcggca atttgaatgt tcactttatc ggcatcaggt
     1621 ataccggttt tgctcagatg ctcctgcacc ttcttcgcct gatcgtcttt atcgtgctga
     1681 cctgtaaccg catcccagag tttttctccg gcatctttca caaaattgaa cagacccata
     1741 gctacctcat tcgttaacgg aaacactcag aaagtgtagc agaggaacgc ggcgcgttaa
     1801 cgccgcgggg gtgagcacag gtttaatcgc gggtttgcac ccagaacgcg tgaatcaaac
     1861 cggggatata cccaagcagt gtcagcagaa tattaataat gaacgcccaa ccgaaccctt
     1921 taccgagcag cacgccgagc ggcggcagaa tgatggtgat gacgattctc cagaaaccca
     1981 tatgtactcc ctataagaaa attactcatt gtttaaaaag agattttatc tcttaagcgt
     2041 agtaatttta gcggagcctg ccagtttcag cttacgcttt tactctcatt tacgctgctg
     2101 aggctggtat atgttgcatt tttagtttag agtttactta atttagaaaa tacttaaata
     2161 aatatgactg aactcgcgca attacaggcc agtaccgaac aggcagcggc cttattgaaa
     2221 gcaatgagcc accctaaacg gttgctgatt ctgtgcatgc ttagcggttc ccccggcacc
     2281 agcgcgggag agctgacgcg cattaccgga ctgagcgcct ctgcgacatc acagcatctc
     2341 gctcgtatgc gggacgaagg gcttatcaac agccaacggg atgcccaacg cattctatat
     2401 tccattaaaa atgaggcggt aaatgccatt atcgccaccc tgaaaaatgt ctattgtccg
     2461 taaggagtca ctatggcttt gacaaccatt tcgccgcatg atgcacaaga attaatcgca
     2521 cgcggcgcaa agttaatcga tattcgtgac gctgatgaat atcttcgtga acatattcct
     2581 gaagcagatc tggctccatt atccgtgctg gaacagtcag gtcttccacc taaattacgt
     2641 cgcgagcaaa ttatattcca ctgccaggca ggtaaacgca ccagtaataa cgccgataaa
     2701 ttagcagcga ttgccgcccc cgcagaaata tttttactcg aagatgggat tgatggctgg
     2761 aaaagagctg gattgccagt agcggtaaat aaatctcaac ccttgccgtt aatgcgccag
     2821 gtgcagatcg ctgcgggtgg tttaatatta atcggcgttg tactgggtta taccgtaaat
     2881 agcggtttct tcttattaag tggctttgtt ggtgccgggt tactgtttgc aggaatcagc
     2941 ggtttttgtg gaatggcaag gttgttagat aagataccgt ggaaccaacg agcttgagaa
     3001 gcgacgccgg acgcgcccta gcagcgacat ccggcctcag taattagatc aggaaatcgt
     3061 cgagagattt accttctgcc agcgcctgag caattggctt cggtgtacgg ccctgaccgg
     3121 tccaggtttt agtttcaccg ttaacatcag tgaatttata tttcgccgga cgcggctggc
     3181 gttttttacc agcacgtggt gcagcagcag agctattacc caataactct tccgggttaa
     3241 ttccgtcagc tttcatcagc tccagccagg tgctaatttt ttcctgacgc tcagccagtt
     3301 cacgctgctg ctgttcttct tcttcacgtc tttctttagt gacaaccctg aatttttcga
     3361 gcatttcttc aagaacgtca atggagaatt cgcgagccat cgcacggagg gtgcgaatgt
     3421 tatttaaact ttgtaacatt acggacataa taaagaaaac cttttaacgc caaaacaaaa
     3481 aagtatttcg tcgcgtagtg taagcgatca cttattatat ttccacaaaa tttaatctat
     3541 ttacagtcct gcgcgagatt atttcagctg ttattttctg aaactctcag aataatttca
     3601 agattatata ttagccattt tgatgagtat tttaggttat tggtgaacag agtggcgctc
     3661 tctgctcgct aactccgtaa gcctgacaaa accgaagtga ataaaaatgc aatataacag
     3721 attaaaagaa aacacccctc actggtgctt tctgctgtta atgtacattt tctggcatca
     3781 aaaatgctgt aaatgacccg gtcacgacaa acaccgaatt ctctacgctt atagcgttac
     3841 ctccccccca aactttcatt agtgaaatta aaggcaaggc catatccaat tgatttaatt
     3901 gaaaataaat catatcggca tgtaaaaatc cacaatgtac aaaaaacgaa ccgttacgga
     3961 atattttatc tacaaaagct gactaaataa aaatttttca ctaatttatt agtcatagcc
     4021 agcgatatac gctatgcgaa aatacagatg gcaatgagat ccactgcttt catctccatt
     4081 aacatcccat tacgctttta ttaaggagca ttagcatgtt ctcaccgcag tcacgcttgc
     4141 gtcatgcagt tgcagatacg ttcgcgatgg ttgtttactg ttctgtcgtg aacatgtgta
     4201 ttgaagtttt cctctccgga atgagcttcg aacagtcttt ttattccaga ttggtagcga
     4261 ttccggtgaa catcttaatt gcatggccat acggtatgta ccgcgatctg tttatgcgcg
     4321 cggcacgcaa aggctggata aaaaatctgg ctgatatcct ggcttatgtg acgttccagt
     4381 caccggtgta tgtggcgatc ttgttagtgg tgggcgcaga ctggcatcag attatggcgg
     4441 cggtcagttc aaacatcgtt gtttcgatgt tgatgggggc ggtttatggc tacttcctcg
     4501 attattgccg ccgactgttt aaagtcagcc gttaccagca ggtaaaagcc tgactcttct
     4561 tttcgcgact ggcatcgcca gtcgcgtctt taactggctt cgccaaataa accattcaaa
     4621 taacgttcaa gcgcaacacg cgaactaaag ccatgcggga taccgtaatg ctcgtgtcgc
     4681 tgtcgctcgc ctgctaaata gagcggaaat tgctggtaat gatcgcaagt tttaatatcg
     4741 gaagcgggtt cagccagtgt tgaactgcgt tcttttaatg ttggatgaat taccagagcg
     4801 gtacgtccca ttcgcgcttc gcgattaaca tagacataat tttccccacg gcggtaacca
     4861 tacgctttct gggttaccac atcgacagta aaaccgactt tttcaagtac gcgcgccacc
     4921 tcgtctggtc gtaaatacat atttgatcct cgttattttc aaccgcgggc ctaccttacc
     4981 tgattgcgca ttcacaacgc tttcagaaaa gtccataaag ccgcgatgaa cctcagttaa
     5041 gaaaattatg gtctacactg aaaattacat cgaattctaa tgggggatca tatgtttaac
     5101 cgaccgaacc gcaacgacgt tgatgatggc gtgcaggata tccagaatga tgtcaatcaa
     5161 ttagctgaca gcctcgaatc tgtattgaag tcctggggca gcgacgccaa aggggaagct
     5221 gaagccgcac gcagtaaagc tcaggcgttg ctgaaagaaa ccagggcgcg aatgcatggt
     5281 cgtactcgcg tccagcaagc cgcgcgcgat gccgttggct gcgcggattc ttttgttcgt
     5341 gaaagaccct ggtgtagcgt gggtacagca gctgcggtag gtatttttat cggcgcacag
     5401 ttaagcatgc gcaaatcgta gtgcaaaaat gataataaat acgcgtcttt gaccccgaag
     5461 cctgtcttcg gggtttcttt ttgcctggtg aatcacaaaa atccccctcc cccgtcacgc
     5521 tcatatccag ggtaatttcg atcactattt gctatatatt gtgtggttga atcttttttc
     5581 aactacatct agtatctctg tatcaacaga gagacaaccc gacgcgtatc atcgcgccgt
     5641 atcttcattt taaacagaaa tacgaatcat gcgcataact atttatactc gtaacgattg
     5701 cgttcagtgc cacgccacca aacgggcgat ggaaaaccgg ggctttgatt ttgaaatgat
     5761 taatgtcgat cgcgttcctg aagcagcgga agcgttgcgt gctcagggct ttcgtcagtt
     5821 gccggtagtg attgctggcg atcttagctg gtctggtttc cgtccggaca tgattaaccg
     5881 tctgcatcca gcgccacacg cggccagtgc atgagccagc tcgtctactt ctccagcagc
     5941 tccgaaaaca cgcagcgttt tatcgaacgt ttaggtctgc ccgcggtgcg catcccgctc
     6001 aatgagcggg aacggattca ggtagacgag ccttacatcc tgatcgtgcc ctcttacggc
     6061 ggcggcggta cggctggcgc ggtgccacga caggtaattc gctttttaaa cgacgagcac
     6121 aaccgggcgt tgcttcgcgg cgttattgct tctggtaatc gcaactttgg tgaggcgtat
     6181 ggccgcgccg gagatgtgat tgcccggaaa tgcggcgtgc cgtggctgta ccgttttgaa
     6241 ctcatgggta cgcaaagcga tatcgaaaac gttcgtaaag gagtaaccga attttggcaa
     6301 cgacaaccgc agaatgcctg acgcaggaaa cgatggatta ccacgcgctg aatgcgatgc
     6361 ttaatcttta cgatagcgca ggtcgcattc agttcgataa agaccgccag gccgttgacg
     6421 cctttattgc gacgcatgtg cgtccgaaca gtgtgacctt cagtagccag cagcagcgcc
     6481 tgaactggct ggtcaacgaa ggttactatg atgaaagcgt tcttaatcgc tactctcgcg
     6541 actttgtcat tacgctgttt gcccacgcac acaccagcgg ttttcgtttc cagacattcc
     6601 tcggggcatg gaagttttac accagctata cgttgaagac attcgacggt aaacgttatc
     6661 tggaagattt tgccgatcga gtcacgatgg tggcgctgac gctggcacaa ggcgatgaga
     6721 cgctggcgtt gcaactgacc gatgaaatgc tgtcaggacg ctttcagcca gccacgccaa
     6781 cattcctcaa ctgcggtaag cagcagcgcg gcgaactggt ttcctgtttt ttgctgcgta
     6841 ttgaagacaa tatggagtcg attggtcggg cggtaaattc cgcactgcag ctgtcgaaac
     6901 gcggcggcgg cgtagcattt ttgctgtcga atctgcggga agcgggcgcg ccaattaaac
     6961 gtattgaaaa tcaatcttct ggcgtaattc cggtgatgaa aatgttggaa gacgcatttt
     7021 cctatgccaa ccaactcggc gcgcgtcagg gggcaggtgc agtctattta catgctcatc
     7081 atcccgatat tctgcgtttt ctcgacacga aacgggaaaa tgccgacgaa aaaatccgca
     7141 ttaaaacact gtcgctgggc gtggtgatcc cggatatcac tttccatctg gcaaaagaga
     7201 atgcgcagat ggcgctgttt tcgccctatg acgttgagcg agtttatggc aagccgtttg
     7261 ccgatatcgc cattagcgaa cactatgacg aactggttgc cgatgaacgc atccgcaaaa
     7321 aatacctcaa cgcccgtgat ttcttccagc gactggcaga aatccagttt gagtccggct
     7381 atccctacat catgtatgaa gacacagtaa accgtgctaa ccctatcgcc gggcgcataa
     7441 atatgagtaa tctctgctca gaaattttgc aggttaacag cgcctcagag tatgacgaga
     7501 atctcgacta tgcccgcaca ggccatgata tttcctgcaa tttaggttcg ttgaatattg
     7561 cgcacaccat ggattccccc gatttttccc gcacggtaga gactgccgtg cgcggtttaa
     7621 cggcggtgtc agatatgagt catatccgca gcgtgccgtc catcgaagcc ggaaatgccg
     7681 cctcgcacgc catcggactg gggcagatga atttacatgg ttatctggcg agggaaggca
     7741 tcgcttatgg ttcgccggaa gccctggatt tcaccaatct ctatttctat accatcacct
     7801 ggcacgcact gcgtacctcg atgttgctgg cgcgcgaacg cggtgaaacc ttcgccgggt
     7861 tcaaacagtc acgctatgcc agtggtgaat atttcagcca atatctgcaa ggtaactggc
     7921 agccgaaaac ggcgaaagtt ggcgaactgt ttgcccgcag cggtattacg ttacctaccc
     7981 gtgagatgtg ggcgcaactg cgcgacgacg tgatgcgcta cggcatatac aaccagaatc
     8041 tccaggcggt gccgccaacc gggtctatct cttatatcaa ccatgctacg tcgagtattc
     8101 atccgattgt ggcgaaagta gagatacgca aagagggcaa aacaggacgc gtttactacc
     8161 ctgccccgtt tatgactaac gaaaatctgg cgctgtatca ggacgcttac gaaattggcg
     8221 cagaaaagat catcgacacc tacgcggaag cgactcgcca tgtcgatcag gggctgtcgc
     8281 tgacgctgtt tttccccgat accgccacca ctcgcgatat caacaaagcg cagatttacg
     8341 cctggcgcaa aggtatcaaa acgctctatt acatccgcct gcgtcagatg gcgctggaag
     8401 gcactgaaat tgaaggctgc gtctcctgtg cactttaagg aatatctatg aaactctcac
     8461 gtatcagcgc catcaactgg aacaagatat ctgacgataa agatctggag gtgtggaatc
     8521 gcctgaccag caatttctgg ctaccagaaa aggtgccgct gtcgaacgat attcccgcct
     8581 ggcagacatt aactgtcgta gaacaacaac tgacgatgcg cgtttttact ggcctgacgc
     8641 tgctcgacac gctgcaaaat gttatcggcg cgccttcgct gatgcccgat gcgctcacgc
     8701 ctcatgaaga ggcggtatta tcgaatatca gctttatgga agcggttcat gcccgctctt
     8761 acagttcgat tttctcgacg ctgtgccaga ccaaagatgt cgatgccgcc tacgcctgga
     8821 gtgaagaaaa cgcaccgttg cagcgaaaag ctcagattat tcagcaacat tatcgcggtg
     8881 atgatccgct gaaaaagaaa atcgccagtg tgtttcttga atcttttttg ttctattccg
     8941 gtttctggct gccgatgtat ttttccagcc gcggaaagct gaccaatacc gcggacctga
     9001 tccgtctgat tatccgcgat gaagcagtcc acggttacta cataggctat aaatatcaga
     9061 aaaacatgga aaagatatct ctgggacaac gtgaagagtt gaagagtttc gccttcgatt
     9121 tgttgctgga actctacgac aacgagttgc aatacaccga tgagctgtac gccgaaaccc
     9181 cgtgggctga cgatgtgaaa gcgtttctct gttacaacac caataaggct ttgatgaatc
     9241 tgggctacga accgttattt cccgcagaaa tggcggaagt gaatccggca atcctcgccg
     9301 cgctttcgcc gaatgccgat gaaaatcacg atttcttttc cggttcaggc tcctcttatg
     9361 tgatggggaa agcggttgaa acagaagatg aagactggaa tttctgaggg tgttattttc
     9421 aaaaatatca ctacccgcag cagggaaata attcccgcca aatagctttt tatcacgcaa
     9481 atattctgta gtgatctaca ctgatactct gttgcattat tcgcctgaaa ctacaatatt
     9541 caggcgtttt ttcgctatct atgacaaaaa atatcaactt ttctcgattt gctctcagcc
     9601 cttatatcac ggaaaattcc ggcgatttgc tcgcatcaat attcatgcca catttgccct
     9661 caggggttgc ctcagattct cagtatgtta gggtagaaaa aagtgactat tcccattggg
     9721 taatatatcg acataaacaa ataaaggaat cattctattg catggccatt aaattagaaa
     9781 ttaaaaatct ttataaaata tttggtgagc atccacagcg agcgttcaaa tatatcgaac
     9841 aaggactttc aaaagaacaa attctggaaa aaactgggct atcgcttggc gtaaaagacg
     9901 ccagtctggc cattgaagaa ggcgagatat ttgtcatcat gggattatcc ggctcgggta
     9961 aatccacaat ggtacgcctt ctcaatcgcc tgattgaacc cacccgcggg caagtgctga
    10021 ttgatggtgt ggatattgcc aaaatatccg acgccgaact ccgtgaggtg cgcagaaaaa
    10081 agattgcgat ggtcttccag tcctttgcct taatgccgca tatgaccgtg ctggacaata
    10141 ctgcgttcgg tatggaattg gccggaatta atgccgaaga acgccgggaa aaagcccttg
    10201 atgcactgcg tcaggtcggg ctggaaaatt atgcccacag ctacccggat gaactctctg
    10261 gcgggatgcg tcaacgtgtg ggattagccc gcgcgttagc gattaatccg gatatattat
    10321 taatggacga agccttctcg gcgctcgatc cattaattcg caccgagatg caggatgagc
    10381 tggtaaaatt acaggcgaaa catcagcgca ccattgtctt tatttcccac gatcttgatg
    10441 aagccatgcg tattggcgac cgaattgcca ttatgcaaaa tggtgaagtg gtacaggtcg
    10501 gcacaccgga tgaaattctc aataatccgg cgaatgatta tgtccgtacc ttcttccgtg
    10561 gcgttgatat tagtcaggtg ttcagtgcga aagatattgc ccgccggaca ccgaatggct
    10621 taattcgtaa aactcctggc ttcggcccac gttcggcact gaaattattg caggatgaag
    10681 atcgcgaata tggctacgtt atcgaacgcg gtaataagtt tgtcggcgca gtctccatcg
    10741 attcgcttaa aaccgcgtta acgcagcagc aaggtcttga tgcggcgctg attgatgcgc
    10801 cgttagcagt cgatgcacaa acgcctctta gcgagttgct ctctcatgtc ggacaggcac
    10861 cctgtgcggt gcccgtggtc gacgaggacc aacagtatgt cggcatcatt tcgaaaggaa
    10921 tgctgctgcg cgctttagat cgtgaggggg taaataatgg ctgatcaaaa taatccgtgg
    10981 gataccacgc cagcggcgga cagtgccgcg caatccgcag acgcctgggg gacaccgacg
    11041 actgcaccgg ctgacggcgg tggtgctgac tggctgacca gtacgcctgc gccaaacgtc
    11101 gagcatttta atattctcga tccgttccat aaaacgctga tcccgctcga cagttgggtc
    11161 actgaaggga tcgactgggt tgttacccat ttccgtcccg tcttccaggg cgtgcgcgtt
    11221 ccggttgatt atatcctcaa cggtttccag caattgctgc tgggtatgcc agcgccagtg
    11281 gcgattatcg ttttcgctct catcgcctgg cagatttccg gggtcggaat gggcgtggcg
    11341 acgctggtct cgctgattgc catcggcgca atcggtgcct gttcgcaggc aatggtgact
    11401 ctggcgctgg tgttaactgc cctgctgttc tgtatcgtca tcggtttgcc gttggggata
    11461 tggctggcga gaagtccgcg agcggcgaaa attattcgtc cactgcttga tgccatgcag
    11521 accacgccag cgtttgttta tctggtgcca atcgtcatgc tatttggtat cggtaacgtg
    11581 ccgggcgtgg tggtgacgat catctttgcg ctgccgccga ttatccgtct gaccattctg
    11641 gggattaacc aggttccggc ggatctgatt gaagcctcgc gctcattcgg tgccagcccg
    11701 cgccagatgc tgttcaaagt tcagttaccg ctggcgatgc cgaccattat ggcgggcgtt
    11761 aaccagacgc tgatgctggc cctttctatg gtggtcattg cctcgatgat tgccgtcggc
    11821 gggctgggtc agatggtact tcgcggtatc ggtcgtctgg atatggggct tgccaccgtt
    11881 ggcggcgtcg ggattgtgat cctcgccatt atcctcgatc gcctgacgca ggccgttggg
    11941 cgcgactcac gcagtcgcgg caaccgtcgc tggtacacca ctggccctgt tggtctgctg
    12001 acccgcccat tcattaagta actctgcact tgcccggtga cgccgggcat tatcaccctg
    12061 ccaaaaaaag gaataacaat gcgacatagc gtactttttg cgacagcgtt tgccacgctt
    12121 atctctacac aaacttttgc tgccgatctg ccgggcaaag gcattactgt taatccagtt
    12181 cagagcacca tcactgaaga aaccttccag acgctgctgg tcagtcgtgc gctggagaaa
    12241 ttaggttata ccgtcaacaa acccagcgaa gtggattaca acgttggcta cacctcgctt
    12301 gcttccggcg atgcaacctt caccgccgtg aactggacgc cactccatga caacatgtac
    12361 gaagctgccg gtggcgataa gaaattttat cgtgaagggg tatttgttaa cggcgcagca
    12421 cagggttacc tgatcgataa gaaaaccgcc gaccaataca aaatcaccaa catcgcacaa
    12481 ctgaaagatc cgaagatcgc caaactgttc gataccaacg gcgacggtaa agcggattta
    12541 accggttgta accctggctg gggctgcgaa ggtgcgatca accaccagct tgccgcgtat
    12601 gaactgacca acaccgtgac gcataatcag gggaactacg cagcgatgat ggccgacacc
    12661 atcagtcgct acaaagaggg caaaccggtg ttttattaca cctggacgcc gtactgggtg
    12721 agtaatgagc tgaagccagg gaaagatgtg gtctggttgc aggtgccgtt ctccgcactg
    12781 ccgggcgata aaaatgccga taccaaactg ccgaatggcg cgaattacgg cttcccggtc
    12841 agcaccatgc atatcgttgc caacaaagcc tggaccgaga aaaatccggc ggcagccaaa
    12901 ctgtttgcca ttatgcagtt gccagtggcc gatattaacg cccagaacgc cattatgcat
    12961 gacggcaaag cctcagaagg cgatattcag ggccacgttg atggctggat caaagcccac
    13021 cagcagcagt tcgatggctg ggtgaatgag gcgctggcag cgcagaagta atttttattc
    13081 gggcggataa ggcgttttcg cctcatccgc cgctctgtac acaatgcctg atgcgacgct
    13141 gacgcgtcct atcatgccta caaaccacat cgcacatttc aacaagctat ctttcatccc
    13201 atattcatca acatccgcta ttattgattt ccagcttaat catcacctga tgaacaaaaa
    13261 taatgactaa acctaatcat gagcttagcc cggcgctgat cgtgctgatg tctatcgcca
    13321 ccggtctggc ggtagccagt aactattacg cccagccatt gctcgacacc atcgcgcgta
    13381 acttttccct ttccgccagt tcggcaggct ttattgttac cgccgcgcag ttgggctatg
    13441 ccgcaggtct actgtttctt gttcccctcg gtgatatgtt tgaacgccgc cgcctgattg
    13501 tctcgatgac cttactggca gcgggcggta tgttaatcac tgccagcagt cagtcactgg
    13561 cgatgatgat cctcggtacg gcattaaccg gtttattctc agtcgtggca caaattctgg
    13621 ttccgctggc agcgacgctg gcttcaccgg ataaacgcgg caaagtggtt ggcaccatta
    13681 tgagcggtct gctgttgggg atattgctgg cacgaaccgt tgccgggtta ctggcaaacc
    13741 tcggcggctg gcgcaccgtc ttttgggttg cttcggtgtt aatggcactg atggcgctgg
    13801 cattatggcg tggtctgcca caaatgaaat cagaaaccca cctcaactac ccacagttgt
    13861 tgggttccgt tttcagcatg ttcatcagcg ataagatcct gcgcacccgc gcgttgctgg
    13921 gctgcatgac ctttgccaac ttcagcattc tctggacctc aatggccttt ttgcttgccg
    13981 ctccaccttt taactacagc gatggtgaca ttggtctgtt tgggcttgcg ggagctgccg
    14041 gagcgttggg cgctcgtccg gcgggcggtt ttgccgacaa gggcaaatcg caccacaact
    14101 ttcggtctgc ttctgctgtt actttcatgg ctggcaatct ggtttggtca cacttccgta
    14161 ctggcgttga ttatcggcat tctggtgctg gacctcaccg tacagggcgt gcatatcact
    14221 aaccagacgg taatttatcg aatacatcct gatgcgcgta atcgcctgac cgcaggttac
    14281 atgaccagct actttattgg cggtgccgcc ggttcgctaa tttcagcctc agcctggcaa
    14341 catggcggtt gggctggcgt ttgtctggct ggcgcgacga ttgccctggt taacttactg
    14401 gtctggtggc gaggttttca tcgtcaggaa gccgcaaatt aagcaatcag caaccgtttt
    14461 cgtggcttta cacttataag ggtgttaaga agcccatcag tctgataagg ttaagatatt
    14521 cattcagtct atttataata ttaacaatcg ttaagcgtac actctatgga aagccctact
    14581 ccacagcctg ctcctggttc ggcgaccttc atggaaggat gcaaagacag tttaccgatt
    14641 gttattagtt atattccggt ggcctttgcg ttcggtctga atgcgacccg tctgggattc
    14701 tctcctctcg aaagcgtttt tttctcctgc atcatttatg caggcgcgag ccagttcgtc
    14761 attaccgcga tgctggcagc cgggagtagt ttgtgggttg ctgcactgac cgtcatggca
    14821 atggatgttc gccatgtttt gtatggcccg tcactgcgta gccgtattat tcagcgtctg
    14881 caaaaatcga aaactgccct atgggcgttt ggcctgacgg atgaggtttt tgccgccgcc
    14941 accgcaaaac tggtacgcaa taatcgccgc tggagcgaga actggatgat cggcattgcc
    15001 ttcagttcat ggtcatcgtg ggtatttggt acggtaatag gggcattctc cggcagcggc
    15061 ttgctgcaag gttatcccgc cgttgaagct gcattaggtt ttatgcttcc ggcactcttt
    15121 atgagtttcc tgctcgcctc tttccagcgc aaacaatctc tttgcgttac cgcagcttta
    15181 gttggtgccc ttgcaggcgt aacgctattt tctattcccg tcgccattct ggcaggcatt
    15241 gtctgtggct gcctcactgc gttaatccag gcattctggc aaggagcgcc cgatgagcta
    15301 tgaggttctg ctgcttgggt tactggttgg cgcggcgaat tattgcttcc gctatttgcc
    15361 gctgcgcctg cgtgtgggta atgcccgccc aaccaaacgt ggcgcggtag gtattttgct
    15421 cgacaccatt ggcatcgcct cgatatgcgc tctgctggtt gtctctaccg caccagaagt
    15481 gatgcacgat acacgccgtt tcgtgcccac gctggtcggc ttcgcggtac tgggtgccag
    15541 tttctataaa acacgcagca ttatcatccc aacactgctt agtgcgctgg cctatgggct
    15601 cgcctggaaa gtgatggcga ttatataacc cacaagaatc atttttctaa aacaatacat
    15661 ttactttatt tgtcactgtc gttactatat cggctgaaat taatgaggtc atacccaaat
    15721 ggatagttcg tttacgccca ttgaacaaat gctaaaattt cgcgccagcc gccacgaaga
    15781 ttttccttat caggagatcc ttctgactcg tctttgcatg cacatgcaaa gcaagctgct
    15841 ggagaaccgc aataaaatgc tgaaggctca ggggattaac gagacgttgt ttatggcgtt
    15901 gattacgctg gagtctcagg aaaaccacag tattcagcct tctgaattaa gttgtgctct
    15961 tggatcatcc cgtaccaacg cgacgcgtat tgccgatgaa ctggaaaaac gcggttggat
    16021 cgaacgtcgt gaaagcgata acgatcgccg ctgcctgcat ctgcaattaa cggaaaaagg
    16081 tcacgagttt ttgcgcgagg ttttaccacc gcagcataac tgcctgcatc aactctggtc
    16141 cgcgctcagc acaacagaaa aagatcagct cgagcaaatc acccgcaaat tgctctcccg
    16201 tctcgaccag atggaacaag acggtgtggt tctcgaagcg atgagctaac gcgtcatctc
    16261 gctcaaaaat ccagatttat aaaagaaaaa atgaccggcc agcatcgcaa catgctggcc
    16321 tttttggcaa gcaggtcggc tcagccgatg agttaagaag atcgtggaga acaatatgag
    16381 cgcaaatgcg gagactcaaa ccccgcagca accggtaaag aagagcggca aacgtaagcg
    16441 tctgctcctc cttctcacct tgctctttat aattattgcc gtagcgatag ggatttattg
    16501 gtttttggta ctgcgtcatt tcgaagaaac cgatgacgca tacgtggcag ggaatcagat
    16561 tcaaattatg tctcaggtgt ctggcagcgt gacgaaagtc tgggccgata acaccgattt
    16621 tgtaaaagaa ggcgatgttc tggtcactct cgacccgaca gatgctcgcc aggcgtttga
    16681 aaaagccaaa actgcactgg cttccagcgt tcgccaaacc caccagctga tgattaacag
    16741 caaacagttg caggcgaata ttgaggtgca aaaaatcgcc ctcgcgaaag cacaaagcga
    16801 ctacaaccgc cgtgtgccgc tgggcaatgc caacctgatt ggtcgcgaag agctgcaaca
    16861 cgcccgcgac gccgtcacca gtgcccaggc gcaactggac gtcgctattc aacaatacaa
    16921 tgccaatcag gcgatgattc tggggactaa actggaagat cagcctgccg tgcaacaggc
    16981 tgccaccgaa gtacgtaacg cctagctggc gctggagcgt acccgtattg tcagtccgat
    17041 gaccggttat gtttcccgtc gtgccgtgca accgggtgcg caaattagcc caacgacgcc
    17101 gctgatggcg gtcgttccag ccaccaatat gtgggtggat gccaacttta aagagacgca
    17161 gattgccaat atgcgcatcg gtcagccggt caccatcacc accgatattt acggcgatga
    17221 tgtgaaatac accggtaaag tggttggtct ggatatgggc acaggtagcg cgttctcact
    17281 gcttccggcg caaaatgcga ccggtaactg gatcaaagtc gttcagcgtc tacctgtgcg
    17341 tatcgaactg gaccagaaac agctggagca atacccgctg cgcattggtt tgtccacgct
    17401 ggtgagcgtc aataccacta accgtgacgg tcaggtactg gcaaataaag tacgttcaac
    17461 accggtcgcg gtaagtaccg cgcgtgaaat cagcctagca cctgtcaata aactgatcga
    17521 cgatatcgta aaagctaacg ctggctaatc cagaggtgcg tgtgatgcaa cagcaaaaac
    17581 cgctggaagg cgcgcaactg gtcattatga cgattgcgct gtcactggcg acattcatgc
    17641 aggtgctgga ctccaccatt gctaacgtgg cgatccccac tatcgccggg aatctgggct
    17701 catcgctcag ccaaggaacg tgggtaatca cttctttcgg ggtggcgaat gccatctcga
    17761 tcccgcttac cggctggctg gcaaagcgcg tcggggaagt gaaactgttc ctttggtcca
    17821 ccatcgcctt tgctattgcg tcgtgggcgt gtggtgtctc cagcagcctg aatatgctga
    17881 tcttcttccg cgtgattcag gggattgtcg ccgggccgtt gatcccgctt tcgcaaagtc
    17941 tattgctgaa taactacccg ccagccaaac gctcgatcgc gctggcgttg tggtcgatga
    18001 cggtgattgt cgcgccaatt tgcggcccga tcctcggcgg ttatatcagc gataattacc
    18061 actggggctg gatattcttc atcaacgtgc cgattggcgt ggcggtggtg ttgatgacac
    18121 tgcaaactct gcgcggacgt gaaacccgca ccgaacggcg gcggattgat gccgtggggc
    18181 tggcactgct ggttattggt atcggcagcc tgcagattat gctcgaccgc ggtaaagagc
    18241 tggactgttt ttcatcacag gaaattatca ttcttaccgt ggtggcggtg gtggctatct
    18301 gcttcctgat tgtctgggag ctgaccgacg ataacccgat agtcgatctg tcgttgttta
    18361 agtcgcgcaa cttcaccatc ggctgcttgt gtatcagcct cgcgtatatg ctctacttcg
    18421 gcgctattgt tctgctgccg cagttgttgc aggaggtcta cggttacacg gcgacctggg
    18481 caggtttggc ctctgcgccg gtagggatta ttccggtgat tctgtcgccg attatcggcc
    18541 gcttcgcgca taaactggat atgcggcggc tggtaacctt cagctttatt atgtatgccg
    18601 tctgcttcta ctggcgtgcc tatacctttg aaccaggtat ggattttggc gcgtcggcct
    18661 ggccgcagtt tatccagggg tttgcggtgg cctgcttctt tatgccgctg accaccatta
    18721 cgctgtctgg tttgccaccg gaacgactgg cggcggcatc gagcctctct aactttacgc
    18781 gaacgctggc ggggtctatc ggcacgtcga taaccacgac catgtggacc aaccgcgagt
    18841 cgatgcacca tgcgcagttg actgagtcgg taaacccgtt caacccgaat gcccaggcga
    18901 tgtacagtca actggaaggg cttgggatga cgcaacagca ggcatcaggc tggattgccc
    18961 agcagatcac caatcagggg ctgattattt ccgccaatga gatcttctgg atgtcagccg
    19021 ggatattcct cgtactgctg gggctggtgt ggtttgctaa accgccattt ggcgcaggtg
    19081 gcggcggagg cggtgcgcac taagtacaac taagccagtt catttgaact ggcttttttc
    19141 aattaattgt gaagatagtt tactgactag atgtgcagtt cctgcaactt ctctttcggc
    19201 agtgccagtt cttcgttgct gttgatgcgt acgtcacgtt ccagaatgct acgcgcaata
    19261 tcctgcgctt cctgcaacga gtgcatctgg taagtgccac actggtagac gttcagttcc
    19321 gggatctgat tctgatcctg cactttcagc acgtcttcca ttgccgcttt ccaggcatca
    19381 gcaacacgct gctcatctgg cgtaccaatc agactcatat aaaaaccggt gcggcagccc
    19441 attggcgaga tatcgataat ctctacacca ttaccgttaa gatggttacg cataaaacca
    19501 gcaaacaggt gctccagggt atggatccct ctttctggca tcacttcttt gttcggcacg
    19561 cagaagcgca gatcgaacac ggtgattgcg tcgccatgcg gggtgttcat tgttttcgcc
    19621 acccgaactg caggcgcttc catccgggta tgatcgactg tgaagctatc taacaacggc
    19681 atttagccac ctccggtaat ttttttaaaa attttctgaa ctctttcttc ccaggcgagt
    19741 ctgagtatat gaaagacgcg catttgttat catcatccct gaattcagag atgaaatttt
    19801 ggccactcac gagtggcctt tttcttttct gtcaggcgtg tttttccagc cacaccgcaa
    19861 acggttcggt atcagcggtt tccatttcct gctgacggcg ttcagatgcc tcgcgctcgg
    19921 ctacaaaatc ctcttcgcgc agaatttcca gcggctcttc acgcagcaga ttacggtagg
    19981 cttctgcaaa cgctttgcct gttccgccaa taccagtatc aatcatagac cttaagatac
    20041 gggcagagaa agtcagatcg ggattatcga agcaggcaac cagttcatca cacactttct
    20101 gatacgcttc gccgccgtta atgctatcca gcgtttgcgc gacgcgtttc agatcgtgga
    20161 acagatcttt acccacctgc agtaacggga actgcgcggt ttcgcagccg atacccagcg
    20221 tcaggcccgg tttgcgacct tcaaggatca cccggttcca gttaacgcgc gtacaggcaa
    20281 gttcgctact gctcatttcc ggtgcatcag ccagcgcaca ccagaccata aacaggtcga
    20341 ggaatcgcac ctgctgttca tctacaccaa tcggcgagaa cgggttgatg tccagcgaac
    20401 gcacttcaat atattcaatg ccgccacgta acagcgcatc agaaggtgac tcgccgctgc
    20461 gggtaacgcg ttttggacga atcggcgcat acagttcgtt ttcaatctgc agcacgttgc
    20521 tattgatttg cagcctctta ccgtctttct caataccaat cttcgcgtac tcttccgatg
    20581 gcgttttgat tgcctgttta aggcccgcta cgtactcgta aagatcgttg aaggtaatac
    20641 caagattgct ttgcgattta ttggtatagc cgagatcgct caaacgaaga gaggtcgcat
    20701 acggcaggta atacataccg cactcggttt tctcaaacgg cagcgacgtt ggttttcctt
    20761 gcaggaaaga agaacaaatc gccggagatg caccaaacag ataaggaatg acccaaccga
    20821 aacgatagta attgcggata acgcggaaat agcccgcaga aattttctct ttggcatcag
    20881 cgcccgagat atcaccgcac ttcgcttgcc agaatgccat tggcaaagag aaattgtagt
    20941 gcacgccgga aatggtttgc atcagcgcgc cgtagcgatt tttcagccct tcacgataca
    21001 gcgttttaaa gcgtccggtg ttagaagtgc cgtactgtgc cagttcgatg tcctgacctt
    21061 ctgcgatgta gcatggcata cttaacggcc acatccgctc atcgcccata ttgcgcgccg
    21121 tataacgatg cagatcgcgc ataaaggtca gcatatgttc aatatcacca tccactggtg
    21181 taatgaattc cagcaatgct tccgcaaaat cggtagtaat ccatttgtgc gtcagtgcgg
    21241 aacctaatgc ttcaggatga cctgttgttg ccagtgtgcc atcagcatta acacgcaaag
    21301 tttcgcgctc cagcccacgc tgtatcccct ttaacgcctg aggatgtttt tccagccagg
    21361 ccagcgcctg tgatacgtcc gggatcaaat tgacctcccg cctgtcaaaa tcgttttaat
    21421 tagcataact gtaatggtga ccatatgtgc aggcctacaa ttagtgccac cacatcatgc
    21481 cctgaacggt cgctgctgca actgcaacat agcgtaacgc tttaccaagg cacaaaaaaa
    21541 agattactgg cccccaggag atgcgcatcc atcccgctaa cagacacagt aaatcgccaa
    21601 ccacgggcat ccagcttaat aatagcgtga ctgcaccata gcgtttcagc cagccggtag
    21661 ctttctcttg ccagcgcgat gttttacgca atggaaagaa acgcccaagg ataacgttag
    21721 ttaaccctcc aaggctatta cccattgttg ctgttaaaac taaaacctag ggatgactga
    21781 tcccggaaag caacattgcc accagcacga cttcggagtt gccgggtaat agtgtagcgc
    21841 tgagaaaact actggcaaac aacgagaaaa gcgataacgc ttcactcaca gcaagcgaac
    21901 atccacagcg tccatgcctg ctgcacgggc cgcctgaata ccgaaatcgg catcttcaaa
    21961 gaccacacac tgcgtcggtt gcacgcccat acgctgcgcg cacaacaaaa atgtgtctgg
    22021 cgcgggttta tggtgtttga cgtgatcagc agcgacgacg gcgtcaaaat aacggcgtaa
    22081 tcccaggtgc gccagcaatg cctctgcgat ggcgctttca ctccccgttc ctacagccat
    22141 tgggcgacga ccatgccaac ttttcaccac ttcaacaaga ggaagcggct cgacactatc
    22201 cagcagcata cttcttactg cttctgtttt ttcacgcgct aacgcatgcg ggtcgagatc
    22261 ggcctgattc agctcaataa ttgcctgagc aatacgccag gtgggcgatc cattaagcgc
    22321 aatcatcgcc tgaatatcgt actgaagacc gtagtgccct aatacttcgc gccacgcttt
    22381 acggtgcgta ggctccgtat ccaggattgt gccatccata tcaaaaatta aacctgcata
    22441 acgctcgtac atggtcttct cgcaagtcga aaaatatgac gctactttag cgtaattgct
    22501 tgattttgtc gctgatggcg aaggagaaga aaattcagga aatggataaa gtagttattt
    22561 agaaatgaga tatttttgaa ggaaattttt tggagaa
//
LOCUS       DADMGC010000062        22319 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.062, whole genome shotgun sequence.
ACCESSION   DADMGC010000062 DADMGC010000000
VERSION     DADMGC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.062"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(21..692)
                     /gene="queE"
                     /locus_tag="JNZ73_002307"
     CDS             complement(21..692)
                     /gene="queE"
                     /locus_tag="JNZ73_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HAY8732568.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            831..971
                     /locus_tag="JNZ73_002308"
     CDS             831..971
                     /locus_tag="JNZ73_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732569.1"
                     /translation="MSEENKENGFNHVKIFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            985..1857
                     /locus_tag="JNZ73_002309"
     CDS             985..1857
                     /locus_tag="JNZ73_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HAY8732570.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTGLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVSTTKNETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     PANPSESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDL
                     FLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1917..3215)
                     /gene="eno"
                     /locus_tag="JNZ73_002310"
     CDS             complement(1917..3215)
                     /gene="eno"
                     /locus_tag="JNZ73_002310"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAY8732571.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="JNZ73_002311"
     CDS             complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="JNZ73_002311"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HAY8732572.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVVGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(5168..5959)
                     /gene="mazG"
                     /locus_tag="JNZ73_002312"
     CDS             complement(5168..5959)
                     /gene="mazG"
                     /locus_tag="JNZ73_002312"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HAY8732573.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(6055..8289)
                     /gene="relA"
                     /locus_tag="JNZ73_002313"
     CDS             complement(6055..8289)
                     /gene="relA"
                     /locus_tag="JNZ73_002313"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HAY8732574.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LYGS"
     gene            complement(8337..9638)
                     /gene="rlmD"
                     /locus_tag="JNZ73_002314"
     CDS             complement(8337..9638)
                     /gene="rlmD"
                     /locus_tag="JNZ73_002314"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAY8732575.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9695..12451
                     /gene="barA"
                     /locus_tag="JNZ73_002315"
     CDS             9695..12451
                     /gene="barA"
                     /locus_tag="JNZ73_002315"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HAY8732576.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12683..14023)
                     /gene="gudD"
                     /locus_tag="JNZ73_002316"
     CDS             complement(12683..14023)
                     /gene="gudD"
                     /locus_tag="JNZ73_002316"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY8732577.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(14044..15384)
                     /locus_tag="JNZ73_002317"
     CDS             complement(14044..15384)
                     /locus_tag="JNZ73_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY8732578.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFETFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15386..16738)
                     /locus_tag="JNZ73_002318"
     CDS             complement(15386..16738)
                     /locus_tag="JNZ73_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8732579.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGQFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(17172..17621)
                     /locus_tag="JNZ73_002319"
     CDS             complement(17172..17621)
                     /locus_tag="JNZ73_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAY8732580.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17639..18421)
                     /gene="truC"
                     /locus_tag="JNZ73_002320"
     CDS             complement(17639..18421)
                     /gene="truC"
                     /locus_tag="JNZ73_002320"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HAY8732581.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTVHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18421..18750)
                     /locus_tag="JNZ73_002321"
     CDS             complement(18421..18750)
                     /locus_tag="JNZ73_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HAY8732582.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19372..19917)
                     /gene="syd"
                     /locus_tag="JNZ73_002322"
     CDS             complement(19372..19917)
                     /gene="syd"
                     /locus_tag="JNZ73_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HAY8732583.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRMHLASNLAEFLNQLKPLL"
     gene            19985..20833
                     /gene="queF"
                     /locus_tag="JNZ73_002323"
     CDS             19985..20833
                     /gene="queF"
                     /locus_tag="JNZ73_002323"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HAY8732584.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20945..22309
                     /gene="ppnN"
                     /locus_tag="JNZ73_002324"
     CDS             20945..22309
                     /gene="ppnN"
                     /locus_tag="JNZ73_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HAY8732585.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5512 a         5916 c         5292 g         5599 t
ORIGIN      
        1 taacgcttat aaatgtgtaa tcaggcaata tttaaatatt tatgtgtttg catcgacaaa
       61 cgccaattac gcgcaataca ggtttcaatg cacaatcgtg tggcatcatc cttttggcta
      121 atcggctgca gtgcaatgac tcgcggttta tcatcggtca gcgttgccag tagttcatcc
      181 agtgcttcaa tatcgcgtac gcgccccact ggatgcttga tttcgttggc tcgctccagt
      241 gcctgtgaca acacttcata gccgccgcgc atgttcagct ttggcgatac ggtaacccag
      301 gtattcggtg tgcagcgtac ttcatgagtt ccactggttt cgatctggca gctaaaaccg
      361 ttcttttcga gcaggtcagt cagtggcagc aaatcatgaa tgcaaggctc accacccgta
      421 atcaccacat gccgcgcggt atatccctga cgaccaatga cagccagcaa gtcttcactg
      481 ctcgccgccc cccacttatc actctctttg gtcttcgcca gaatgctgaa aagggagact
      541 tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc accaggcaca gccaaccggg
      601 catccctgta aacgaataaa aatggcgggg acgccggtaa agtaaccctc accttgcagg
      661 gtctggaaca tctcgttaat cgggtactgc atagcattct ctgtgaagtg gataattgtt
      721 aattattgca gatcctgccg caacaatcat gtcttattaa cattctgtta catgcagatt
      781 taaagaaaaa tacgaaacat atcgttaata attaaaggga gtaacgtatt atgtcagaag
      841 aaaataaaga aaatggattt aatcatgtca aaatattcac caaaattata tttatttttt
      901 ctgtattagt ttttaatgat aacgaatcta aaattaccga tgccgccgtc aatttattta
      961 tccagattta atgagaaatt tataatgcgc tatttcatac taatgttcac tttcgtatgt
     1021 tcctttgttg cagcccaacc aactattgtc ccacaattac agcaacaagt tacgggtctt
     1081 acgagtagct taaactcaca agaaaagaaa gaactgacgc acaagttaga atctattttc
     1141 aataataccc aagtgcaaat tgctgtatta attgtttcca caaccaaaaa tgagacaatt
     1201 gaacaatatg ctacaagagt ttttgacaat tggcgtttag gagatgccaa acgtaatgat
     1261 gggatactga tcgttgttgc ctggtcggat cgcactgtcc gcatccaggt aggttatggg
     1321 ctggaagaaa aggtaaccga tgctctggct ggagatatca tccgtagcaa catgataccc
     1381 gcatttaaac aacaaaaatt agctaaggga ttagagttag ctataatcgc tttgaataac
     1441 caactcactt cacaacacca atatccggct aatccttcag aaagtgaatc aacgtcttcc
     1501 actgatcatt attactttgc tattttttgg gtatttgcag tgatgttctt ccctttctgg
     1561 ttttttcatc aaggcagtaa tttttgtcgc gcatgtaaaa gtggagtctg tatttcagca
     1621 atccatcttt tagatttatt cctgttctcc gataaaactt tttccattgc tgtattttcc
     1681 ttctttttta ctttcaccat atttatggtc tttacctgtt tatgtgtact tcagaaaaga
     1741 gcatctggta gaagttatca ttcagacaac agcggttccg ctggaggctc aagtggcggc
     1801 ggatttagcg gcgggggagg ttcttctggc ggtggcggcg catccggccg ctggtaactc
     1861 caccataaaa aatgccagcc cgaaggctgg cattatcaaa tcagataaag tcagacttat
     1921 gcctggcctt tgatctcttt acgaccgttg tacggtgctt tttcgcccag agcttcttcg
     1981 atacgaatca gctggttgta tttagcaaca cggtcagaac ggctcataga accagttttg
     2041 atctggcctg cagcagtacc aacagccaga tcagcgatgg tagcatcttc agtttcacca
     2101 gaacggtgag agataactgc agtgtagcca gcatctttcg ccatcttgat tgcagccaga
     2161 gtttcggtca gagaaccgat ctggttgaat ttgatcagga tggagttagc gatacctttt
     2221 tcgatacctt ctttcaggat cttggtgttg gttacgaaca ggtcgtcacc aaccagctgg
     2281 attttgtcgc ccagaacttt ggtctggtat gcgaaaccgt cccagtcaga ttcgtccaga
     2341 ccgtcttcga tagaaacgat cgggtactgt ttggtcagtt cttccaggaa gtgagtgaat
     2401 tcttcagagg tgaacgcttt gttgccttcg ccagccagaa cgtatttacc atctttgtag
     2461 aattcagaag ctgcgcagtc catcgccaga gtgatgtctt tgcccagttc ataaccagca
     2521 gctttaacag cttcagcgat aacagccaga gcttcagcgt tggaacccag gttcggcgca
     2581 tagccacctt cgtcaccaac agcagtgttc atgcctttcg ctttcagaac ttttgccagg
     2641 tgatggaaaa cttcagaacc catgcggatg gcttctttca cagttttcgc gccaaccggc
     2701 tgaatcatga attcctggat atcaacgttg ttgtcagcgt gctcaccacc gttgatgatg
     2761 ttcatcatcg gaaccggcat ggagtatttg cccggagtgc cgttcagttc agcgatgtgc
     2821 tcgtacagcg gcataccttt agcagctgca gcagctttgg cgttagccag agatacagcc
     2881 aggattgcgt tcgcgccgaa tttggatttg ttttcggtgc cgtccaggtc gatcatgatc
     2941 ttgtcaatgc cagcctgatc tttagcatct ttgccaatca gcgcctgagc gatcgggccg
     3001 tttaccgcag caacagcttt ggttacgcct ttacccagga aacgggattt gtcgccatcg
     3061 cgcagttcca gagcttcacg ggaaccagta gaagcacctg acggagcagc tgccataccg
     3121 acgaaaccac cctccagatg tacttcggct tcaacagtcg ggttaccacg ggagtcgatg
     3181 atttcacgac cgatgatttt tacgattttg gacattaggt tttcctcaag tcactagtta
     3241 aactgaaact ccagacaaac aacgcgtacc caaggtacgc gttgccgttc taactttttt
     3301 acttacttcg cctgacgttt ctggaactcg ctggcggctt tcacaaagcc tgcaaacagc
     3361 gggtgaccat cacgtggagt agaagtaaac tccggatgga actggcaagc cacgaaccac
     3421 gggtgattcg gaacttcgat gatctcgacc aactgatcat ccccggaacg gcccacaacg
     3481 cgcagacctg catcttcaat ctgtttcaac agcatgttgt tgacttcgta acggtgacga
     3541 tgacgctcaa caattgtcgg cgcattgtat agctggcgaa ccaggctatc gtcaaccaac
     3601 tggcactgct gtgcgccgag acgcatggta ccgccgagat cgctcttctc gctacgaact
     3661 tcaacgttgc cgttttcatc gcgccactcg gtaatcagcg ccacaaccgg gtacttacag
     3721 tctggcacaa attctgtaga gttggcgttc tccatgttgg caacatggcg agcgtaatca
     3781 attaacgcca cctgcatacc caggcaaatg cccagataag gaatattgtt ctcacgcgca
     3841 aaacgcgcgg tcgtaatcat gccttctacg ccacgatagc cgaaaccgcc aggtacgagg
     3901 attgcgtcca gacctttaag gatttcaacg ccgcgcgttt caacatcttg tgaatcgatc
     3961 agtttgatgt tgacgctgac acgattcttc agcccaccgt gtttcagtgc ttcgatcact
     4021 gatttataag catccggcag ttcaatgtac ttgccgacca taccgatggt gacttcactt
     4081 accggattcg cttcttcgaa gataacctgt tcccattcgg acagatttgc ttccgggcag
     4141 tttaagctga atcgtttaca aatataatcg tccagcccct gagatttcaa caggcccgga
     4201 attttataga tggaatcgac gtctttcaga gaaataaccg ctttttccgg aacattacag
     4261 aacaatgcaa tcttcgcacg ttcgttcgcc ggtacagcgc gatctgaacg acaaatcagg
     4321 atgtcaggct ggataccgat ggagagcagc tcttttacag agtgctgagt cggtttggtt
     4381 ttgacttcac cagacgctgc catgtacggc accagcgtca ggtgcataaa cagagtgtgc
     4441 tcacggccaa tttcaacagc catctggcga atagcttcga ggaacggcaa ggattcgata
     4501 tcacctactg taccgccgat ttctaccagt actacgtcat gaccttcgcc accttccagc
     4561 acgcgctctt tgattgcgtt agtgatgtgc ggaataacct gcacggttgc gccgaggtag
     4621 tcaccgcggc gttctttacg cagaacgtca gagtagatac gacccgtggt gaagttgttg
     4681 cggcggctca ttttggtacg aatgaaacgc tcgtagtgcc ccaggtccaa gtcggtttca
     4741 gcgccgtctt cagtaacgaa cacttccccg tgttggattg ggctcatagt acctggatcg
     4801 acgttgatgt aaggatccag tttcatgatg gtcacattga ggccacgggc ttcaagaatg
     4861 gctgcgaggg aggctgcggc aatgccttta cccagagagg atacgacccc gccggtcaca
     4921 aaaatatagt tcgttgtcat gctgaacctg agaagttagg ttgaaaagac gatggaataa
     4981 ccaagacggg aaagcagtat acccgaacat gacctgtgcc acaaactttc attatccctc
     5041 ctcttcgcca gcgcactatt gaaatcagga gtgagaaaat agcccctttg gggtaaatgt
     5101 ttttgacgca aatcaagcgc ttgtcattta aaaaatgaca caaatggcgc ttgaccgcgt
     5161 aattccctta gagatcaatt tcctgccgtt ttacctgttg ccagacttct tccattgttt
     5221 cgaggtcaac acctgtcatt tccagtccac gcgcggcaac aatacgctcc acttcgcgaa
     5281 aacgacgctc gaatttttcg ttcacttttt gcaatgcgat ttctgctttc gtccctaaat
     5341 ggcgagccag attaaccgtg gcaaacagca ggtcccccat ttcctcctcc agtttagcct
     5401 ggtcgacaac agcctgccgc gcctcgtaca tcacctcgtc gatctcttcg tagactttat
     5461 caaccaccgg gccaagcgtc gtccagtcga agccaacgtt ggcgcaacgt ttctggattt
     5521 tttgcgcccg cattaaagcc ggtaaactac gaggaatatc gtccagcgcc gaatgctgcg
     5581 ctttctgcgc gcgctcttcg gttttgattt gctcccaacg ggcaagcact tcactactgt
     5641 tttcggcaga actatcagca aaaacatgcg gatggcgacg ctctaattta tcgctaatag
     5701 cagcgcaaat atcattaaag tcaaagcgcc cttcttcctg agccatttgc gcgtaaaaca
     5761 ccacctggaa tagcagatcg cccagttcac cgcgaagatc gtcaaaatct tcacgggcga
     5821 tggcgtccag cacttcgtag gtttcttcaa gggtgtaagg cgcaatggtg gcaaatgtct
     5881 gctctttatc ccacgggcag ccgttttccg gatcgcgcag gcgctgcata atagtgagca
     5941 aacggtcgat ttgattcatt gaattgtcct gaaaattgcg ggtctgtcag gtgtaatacc
     6001 ttcgagtaaa agatatcgct ggatacggct gacgccatat ccagctatgt aagactaact
     6061 cccgtacaac cgacgcgcgt cgataacatc cggcacctgg ttgagtttac ccagcacgcg
     6121 ccccagcact tgcaggttgt aaatctcaat ggtcatgtcg atggtcgcca gttgctgttt
     6181 ggtgtcgcta cggctggcaa cgccaagcac gttcaccttc tcgttggcga gaatggtcgt
     6241 gatatcacgt aacaacccac tacgatcatt agccaccacg cggaccacca gcgaatatcc
     6301 ggcggagtag ctctcacccc ataccgcgtc aacaatgcgt tctggcgcat gggagcgcag
     6361 ttccgccagt tgttcgcaat cggcgcggtg tactgaaata ccgcgtccct gggtaatgaa
     6421 gccgacaatc tcatctccag gaatcggctg gcagcagcgc gcgatgtggt gcatcaggtt
     6481 accaacacct tcaaccacca cacgaccgtt atctttactg cggttttgcg gtgtgtagct
     6541 tttttgctga agctgtttca gcgcggcggc gtcctgctct tcggcactcg gcttattaaa
     6601 ttgcgattgc aggaagttca ccatctgatt gagacggata tccccgccac caatcgccgc
     6661 cagcaactcg tcgacatcat tgaagttgta acgcggcagc agatgttttt ctgcttcttt
     6721 caggctgatc cccagatgtt ccagctcgtc gtcaaggatt tgccgcccag ccagaatgtt
     6781 tttgtcacgg tcctgtttac ggaaccaggc gtgaattttc gaacgcccac ggctggttgt
     6841 gacgtaaccg aggtttgggt ttaaccagtc acggctgggg ttcggctgtt tctgggtgat
     6901 aatttcaatc tggtcgccca tttgcagctg gtaggtgaac ggcacaatgc gcccgccaat
     6961 ttttgccccg atgcagcggt gtccgacatc actgtggatg tggtaagcga agtccagcgg
     7021 cgttgatccc gcaggcaaat caacgacatc acctttcggc gtaaagacat acacccggtc
     7081 gtcaaacacc tgactacgta cttcgtcgag catttcgccg gaatcagcca tctcttcctg
     7141 ccacgcaatc agtttacgca gccaggcaat ccggtcttca tgtcccgaac gtgcgccgcc
     7201 agctgccgcg ccctctttat atttccagtg cgcagcaaca cccaactccg catcttcatg
     7261 catctgtttg gtgcggattt ggatctcaac agtttttcca cccggcccca gaaccacagt
     7321 atgaatagac tgataaccgt ttggtttcgg gttagcgacg taatcgtcaa actcatccgg
     7381 caggtggcga tagtgagtgt gcactatccc cagtgcggca taacaatcct gtaaacgctc
     7441 ggcgacaata cgtaccgcac gcacatcaaa cagctcatcg aaggcgaggt tctttttctg
     7501 cattttgcgc cagatgctgt agatgtgttt cggacgacca tacacttcag ctttaacgcc
     7561 ttcagctttc atctcagcgc gcagatggcc gacaaactct tcgatatagt gttcgcggtc
     7621 gagacggcgt tcatgcagca gttttgcgat gcgtttgtat tcggtcgggt ggagataacg
     7681 gaagcagtaa tcttccagct cccatttcag ttgcccaatc ccaagacggt tcgccaacgg
     7741 cgcgtagata ttggtgcact cttttgccgc cagtacacgt tcatcttccg gcgcatcttt
     7801 tacttcgcgc agatgagcaa tacgctccgc cagtttgatg actacgcagc gaaaatcatc
     7861 gaccatcgcc aataacatcc ggcgaacgtt atcgacctgt tcggaggaaa cagaatcagt
     7921 gtgcgtcgct ttcagctggc ggatcgccgc catatcacgc acgccgtgaa taaggttaac
     7981 aaccgactta ccgacgctct cacgcagcac atcttcgctg actacgttgg catcagccag
     8041 agggaacagc agcgccgccc gcagcgtgtc aatgtccata cttaatgtcg agaggatctc
     8101 caccatctca acaccacgcc acaataacag actggcatcc ggatgcccct gcgtctgttg
     8161 cagacaatac gcccaggttt cggctaagca ctcacacgac ttctggctgg taatacccag
     8221 acttgcgatc catttttccg gatcaaattc accagcctta ttgatatgtg cacttcttac
     8281 cgcaaccatc gtcctctcct ttagggacca gacctgccga aatcggcaaa tcgtaactat
     8341 ttaacgcgcg agaaaagtac catcgattcc agatgtcccg tgtgtgggaa catatccagc
     8401 atcgccagtc gcgcaatggt atatcctgct tttaataacg cttcgctatc ccgagccagc
     8461 gttgcagggt tacaggatac ataaactata cgaataggtt ccagttttat aatttgctgc
     8521 ataacacctg cggcacctgc tcgcgccggg tccagcaaca ctttatcgaa gccgtttttc
     8581 gcccacggct gctttgtgac atcttcttca agattttcgt gataaaacgt cacattctgt
     8641 aagccattaa gacgcgcatt ctgctggcct ttttccacca gcgccggaac accttctaca
     8701 ccgaccacac tggcagcttg tgtcgccaat ggcagtgtaa agttgcccat accgcagaac
     8761 agatccagta cgcgatcttc aggttgcaca tccagccatt ccaacgcacg cgctaccatt
     8821 ttttggttca cacccgcatt gacctgaata aaatcgcgcg ggctaaaagt taagcgcaac
     8881 ccgtttgagt cataccaggg catctcacca gagacggttt cgagtatctc actatcgggg
     8941 gcgagataca gatccaggcc ttcagaatgc gaaaagcgtt ccagtttttc gcgatctgcc
     9001 gaacttagcg gtgcggtatg gcgcaaaatc atcagcgtgc cgctggttgc ctgtaccagt
     9061 tcaacatgac caagatggcg catagcttgt aagctgccca gacatgccct gactttgggc
     9121 agcaatgctt caagttgggg cgctaaaatg gggcattgtt tgacgtcgac aatgtcactg
     9181 gagcccgctt tgcgaaaccc catctgaagt tgctgtgttt tcggtaagta gttcagactt
     9241 aaacgcgcgc ggcggcgata gccccaggga acatcggcga tcacttcaga gacatcgtgt
     9301 ttcattaatc gggcgagtgc cgcacttttg cttcgctgct gtaaatccac gctggcgtgt
     9361 tgttgctgac agccgccgca tacgccaaaa tgaggacagc gtggcgtttc gcgttccggg
     9421 ctatcgctta accggcgtac gactttagcg cgggcatact gttttttatc ttcagtaaca
     9481 gcaacttccg cgttttcctg cggcaataat ccggggataa ataacgtttt gccgttatgt
     9541 cgcgccacgc cctgaccaaa agagtcgagg tcgttgactg aaacggttat gatctgacgc
     9601 gtcgtcgtgc gtcgttttgc agagtagaat tgcgccattg gcgagacttt ctcaatttaa
     9661 cagtgtgacc ttaattgtcc cataacggaa ctccatgacc aactacagcc tgcgcgcacg
     9721 catgatgatt ctgatcctgg caccgaccgt ccttattggt ttattgctga gtatcttttt
     9781 cgtcgtgcat cgctataacg acttgcagcg tcaactggaa gatgccggtg ctagcattat
     9841 tgagccgctt gcagtttcta ctgaatatgg catgagcctg caaaatcgcg aatctatcgg
     9901 tcagttaata agcgtactgc atcgtcgcca ttccgatatt gttcgcgcga tttcggttta
     9961 tgatgaaaat aaccgactct ttgtcacctc caattttcat cttgatccct catcaatgca
    10021 gctcggcagc aacgtgccgt ttcctcgcca gctcactgtc actcgtgacg gcgatattat
    10081 gatcctccgc acgccgatta tttctgagag ttactccccc gacgaatcgc ccagtagcga
    10141 tgccaaaaat agtcaaaata tgttgggata tattgcgctt gagctggatc ttaaatcggt
    10201 tcgcttgcag caatataaag agatctttat ttcctgcgtg atgatgctgt tttgtatcgg
    10261 tattgcgctt atttttggct ggcgcttaat gcgcgatgta accggtccga ttcgcaacat
    10321 ggtgaatacc gtcgaccgca tccgtcgcgg gcaactcgac agccgggtgg aaggatttat
    10381 gctcggcgag ctggatatgc tgaaaaacgg tatcaactcg atggcaatgt cgctggctgc
    10441 ttatcacgaa gagatgcagc acaatatcga ccaggcgacg tccgatctgc gtgaaacgct
    10501 ggagcagatg gaaattcaga acgttgagtt ggatctggcg aaaaagcgcg cccaggaagc
    10561 ggcgcgtatt aaatccgagt ttctggcaaa tatgtcacac gagctgcgta caccactgaa
    10621 tggtgttatt ggctttaccc gcctgacgct gaaaacagaa ttaacaccaa cgcagcgcga
    10681 tcacctgaat acgattgaac gttcggcaaa taatttgctg gcaattatta atgatgttct
    10741 cgacttctcg aaactggaag caggtaagct gattctggaa agtattccat tcccactacg
    10801 cagcacgctg gatgaagtcg ttactctgct ggcacattct tctcacgata aagggttgga
    10861 actgacgctt aatattaaaa gcgacgtgcc tgataacgtg atcggcgacc cactgcgatt
    10921 acagcaaatc atcactaacc tagtggggaa tgcaattaaa ttcaccgaga atggcaacat
    10981 tgatattctg gtagaaaaac gtgcgctgag taataccaaa gtgcagattg aagtgcagat
    11041 tcgggatacc ggcattggta ttcctgaacg cgatcaatcg cgcttattcc aggccttccg
    11101 acaggctgat gccagtattt cccgccgtca tggtggcacc gggctggggc tggtgattac
    11161 acaaaaactg gttaatgaaa tgggcggcga tatttcgttc catagccagc cgaatcgcgg
    11221 ttcaactttc tggttccaca ttaatctcga tctgaacccg aacattatta tcgaagggcc
    11281 atccatccag tgcctcgcag gtaagcgcct ggcctatgtc gaaccaaact ccgcagcagc
    11341 gcaatgcacg ctggatattt taagtgaaac gccgctggaa gtggtttata gcccaacgtt
    11401 ctccgcgctg cctcccgcgc attacgacat gatgttgtta ggcatcgcgg tgaccttccg
    11461 cgagccgcta acaatgcaac atgagcgatt agcgaaagcg gtatcgatga ccgatttcct
    11521 gatgctggca cttccttgcc atgcacaagt caatgctgaa aaactcaagc aagatggtat
    11581 cggcgcgtgt ctgctgaaac cattaacacc tacgcgcctg ttgcctgccc tgacggaatt
    11641 ttgtcatcac aaacaaaaca cgcttttgcc tgtaaccgat gaaagtaagc tggcaatgac
    11701 agtcatggcg gttgatgaca accccgctaa cctgaaactt atcggcgcat tgctggaaga
    11761 tatggtgcaa catgtggaac tttgcgatag cgggcatcag gcagttgaac gggcgaaaca
    11821 gatgccgttc gatttgatct taatggatat tcaaatgccg gacatggatg gcattcgggc
    11881 ctgcgaactc atccaccaac tcccgcatca gcgacaaacg ccggttatcg cggtaacggc
    11941 gcatgcaatg gccgggcaaa aagagaagct gcttggcgca gggatgagcg attatctggc
    12001 gaaaccgatt gaagaagagc gattgcataa tttgttgttg cgctacaagc ctggcagcgg
    12061 tatttcgtct cgcgtcgtga cgcccgaagt caacgaaatt gtggtgaacc cgaatgcgac
    12121 cctcgactgg caactggcac tacgccaggc agcaggaaaa accgatttag cgcgcgatat
    12181 gctgcaaatg ttactcgatt tcctgcctga agttcgcaac aaagttgagg aacagctggt
    12241 tggagaaaac ccggaaggcc tggttgattt gattcataaa ctgcatggca gttgcggcta
    12301 tagcggcgta ccacgtatga agaatctctg ccagctgatc gaacaacagc tacgtagcgg
    12361 tactaaagaa gaagatttgg agccggagct gctggaactg ttggacgaga tggataatgt
    12421 cgcgcgcgaa gccagcaaaa ttctcgggta acggacgtct tgtcgagtag caatcggctt
    12481 tttatgacgc tggtttcaga ttggtgacaa agtgcctgtg tttatgccgg atgcggcgtt
    12541 aacgccttat ccggcctaca aaaccaatta aattcaatga attaaaaaat gatgtagacc
    12601 tgatacatag cacaacaggc tattttgcgt ttagcatcag tctcaaaccg gctccagata
    12661 gagccggttt tggttttctg tcttaacgca ccatgcacgg gcgcttgtta tcgaacgtcc
    12721 agccaggaat cagatactgc attcccatcg catcgtcacg cgcgccaagc ccgtgtttct
    12781 gatacagctc atgggctttc atcacttgat ccatatcgat ttctacaccc agccccggtt
    12841 tttctggcac ctgtaccagc ccgcctttga tctcaaacgg ttctttggtc aggcgctgat
    12901 taccttcctg ccagatccag tgcgtatcaa tagcagtaat tttacccggt gcagcggcgg
    12961 caacatgggt aaacatcgcc agggaaatat cgaagtggtt gtttgagtgt gaaccccagg
    13021 tcaggccaaa ttcatggcac atttgcgcca cacgtaccga accttgcata gtccagaaat
    13081 gcggatccgc cagcgggata tcaacggatt gtaacgagag cgtatggccc atctgccgcc
    13141 agtcggtggc gatcatattg gttgcagtcg gcaggccagt cgcgcgacgg aactctgcca
    13201 tcacttcacg cccggagaaa ccttgctccg caccacacgg atcttctgca taagccagcg
    13261 aacctttcag gtatttacca attttaattg cttcgttcag cgaccaggca ccgttaggat
    13321 cgagcgtaat acgcgcctgc gggaagcgtt gcgccagtgc cacaatagac tcggcttctt
    13381 cttccccggc cagtacaccg cctttcagtt tgaaatcgtt gaagccatat ttttcatacg
    13441 ccgcttccgc caggcgcacc accgcatccg gcgtcatcgc ttcttcatga cgcaggcgat
    13501 accagtcgca tgagtcatcc ggctggcttt gatacggcag aggtgtggct ttgcgattac
    13561 cgacgaagaa cagataaccg agcatttcga cttcgctacg ctgttgacca tcgcccagca
    13621 gcgatgccac gtttaccccc agatgctgcc ccagcagatc cagcattgcc gcttctatcc
    13681 cggtaactac atgaatagtg gtacgcaaat caaatgtctg caaaccgcgc ccactagcat
    13741 cacgatcggc aaaagtatta cgcaccagcg tcagaacgtt tttgtattca cccagcgttt
    13801 tacctaccac cagcggaatc gcatcttcca gcgttttacg gattttctcg ccgccgggaa
    13861 tttcccctac gccagtgtga ccggaattat ctttgataat cacaatatta cgcgtgaaga
    13921 acggtgcgtg tgcaccactc agattcatca gcatactgtc atgacccgcc accgggatga
    13981 cctgcatttc agtaacaaca ggcgtcgtaa attgagaact catatttatg tcctttttca
    14041 gaatcaatga cggccgaaaa cgggacgttt acggtcaaag gtccagccgg ggatcaggta
    14101 ctgcatcgga cctgcgtcgt tacgcgcacc gcaaggcaga cgtttatagg cctcatgtgc
    14161 cttttgtacc tgttcccagt ccagttccac gcccagaccg ggcgcatcag gaacggcaat
    14221 ttttccgttt ttaatctcca gcggattttt ggtcaggcga caatcgccct cctgccaaat
    14281 ccagtgggta tcgatagcgg taggattacc cggtgccgcc gcgcccacat gggtaaatat
    14341 cgccagagag atatcgaaat ggttgttgga atggcagccc caggtcagcc cccagtcgtc
    14401 gcaaagctgc gccacgcgga ctgcaccgga aagcgtccag aagtgcggat cggcaagtgg
    14461 aatatctacc gcattgagca tcaccgcatg acccatttcg cgccagttgg tggcgatcat
    14521 gttagtcgcg acgggcaagc cggtcgcccg tcgaaactcc gccatcactt cacgtccgga
    14581 gaagccctgt tctgcgccgc atggatcttc ggcataggta agaacatcat tcagcccttt
    14641 gcataaagaa atggcttcat caagcagcca tgcaccgttg ggatcaacgg taatccgcgc
    14701 atccgggaag cttttcttca atgcacgaac agtgtcgatt tcttgctcgc caggtaacac
    14761 cccgccctta agtttgaaat ctttaaagcc atagcgatcc tgtgaggctt ccgccagacg
    14821 cacaacggct tcgctgttca tcgctttctg atggcgcaac tgataccact catggttgcc
    14881 cggcgtattt tccagataag gaagatcggt tttggtccga tcacctatat aaaacagata
    14941 accgaggacg gtaatagtct cgcgttgctt gcctggcccc aacagttcgc agaccggaac
    15001 attcagcgcc ttacctagca ggtcaagcaa ggcggcttcc agcgccgcca cggcattaac
    15061 gcgcaattca aaagtccagg cacctttgcc gaaggtctca aaatcggctg cctgatttcc
    15121 tttatgtacc tgctgaacca ctttattcag gcgcgcaact tcctggccca gaaccatcgg
    15181 aatagcatcg accagtgttt gataaatcac ctctccgccc ggcgcttcac caatgccggt
    15241 atgcccggcg ttatcggtga gtaccacgat attgcgggtg aagtatgcgt tatgcgcacc
    15301 accaatattg agcaacatgc tgtcatgtcc ggcgaccgga atgactttca tatcagtaat
    15361 aacaggactg gattgtgtcg ccatcttatt gccccgcgac aggtttcagc tcgatacgct
    15421 tgatatcgcc caccagcacc aggtagctca gtaccgcgat tagggcatga acaccaacat
    15481 aaatcagcgc cccattaaac gagccagtcg tgccaacgat ataaccaatt gcgattggcg
    15541 tgacgatgcc agaaatgtta ccgaacatgt tgaactggcc accggaaaga ccgctgatct
    15601 cttttggcgc ggtatctgcc attactgccc aacccagcgc cccgatgccc ttaccgaaga
    15661 aggccagcgc cataaagccg atgatcatcc actcaacgtt gacgtagttg cagaacacca
    15721 tcaccatcga cagcaacatg cccattacga tcggtgtttt acgcgcaatg ttcagcgatc
    15781 ccgtgcggcg catcagccag tcggaaataa ttccacccag cacaccgccg ataaaaccgc
    15841 aaaccgccgg aacggaagcc acaaagcccg ctttcagaat cgacatccca cgcgcctgca
    15901 ccagataaac cggaaaccag gtaatgaaaa agtaagtcag ggcgttgata cagtactgac
    15961 cgatataaac gccgatcatc atccgtgacc cgagcagctg tttgatctgc ccccacttca
    16021 cgctgaacgg cactttaact ttggtgcttt gctgatccat attgatcagc gcgccacccg
    16081 cggcgatata ctccagctct ttctgattta cccccggatg ttgatttggc tcgtggatga
    16141 ctttcaacca gataaagctg atgacaatgc ccagaccgcc cataaagaag aagacgtgtg
    16201 accagcccac ttcatgcgtc agccagccca taatcggcgc gaagatcacc gttgcgaagt
    16261 attgagcgga gttaaaaatc gacaccgccg ttcccctttc ctgcgccgga aaccaggccg
    16321 caacaatgcg actgttgccg gggaaagatg gcgcttcagc aagcccgacc aggaagcgca
    16381 gagtaaacag ggcaacgata atgccgaatc cactaaagat atcgacgaag ccttgcagca
    16441 aggtaaacat cgaccagata aagatcgacc agaagtagac gcgttttgaa ccaaaacgat
    16501 ccagcaacca accaccaggg atctgcccga taacataagc ccatgagaag gcagagaaca
    16561 catagcccat tcccacggga tcaaggccga tatctttggc catttccgaa ccggcaatag
    16621 aaagcgtagc gcggtcgccg tagttgaagg atgtgacgat aaacaacatc accactatcc
    16681 agtaacgagc attcgtgcgt ttttccacac tgctcgcagc ctgacttaaa gaactcattg
    16741 ttgcactcct gaaaattcgc gttagccacg ctcactctgg actgcgacat cgccaggaaa
    16801 tcagaggtga cgtagggtgt tttttgccgt ttttataggt cgttcgccga atacggcgcg
    16861 tgtttatatc tggcaatagc agtataaaaa gcgcgccata gcggctcacc gtgcaacaac
    16921 acaacattaa tgcgttcaat gaggcccgat tttggcatta gccctggacg gtggaatcca
    16981 cttcacggaa atgaaaacaa gaacaagaaa ggaagggtaa aaacgaagaa ataaagaaga
    17041 gtatgaaatg gatcgcttga ctctaggcaa acgccagtaa aaatccgcgc tatgaagcag
    17101 tttttactgg catttgcctg aaaagattcg attcagcacc gctaaaacga catttaccgc
    17161 tcgctgacat atcaggacaa cagcgtgccc cactgttcga cccacggatt tgattccgtt
    17221 tccggttccg ggttctcact ggcgtcaatc aacagcattt cgccaacccg ctgagcgctc
    17281 tgttcctgca acaaggcatc gaactgtttg ccgccattgc aaaaatttac ataactgctg
    17341 tcgccgagcg caatcacgcc ataacgcaga ttcggctgga agcccagact atctttgatt
    17401 ccctgaaaga gcggcacaat gctatcagga aggtcgccct gcccggtcgt ggacgtaacc
    17461 accagaacat acttatcctg atagggcagc cagtcgctta attcaggatc ttcaaatacc
    17521 gttgctttgt ggccctgtgc ggtcagaatt gcttctgctt cttccgccac taacagcgaa
    17581 tttccgtaca tggtgccgac aaaaatacca atttccgcca taccgttttc tccctggatt
    17641 aggaacttat ctctccatcc tgacccgatg gcgcactgaa ctcaaccctt tcattttcag
    17701 gaagcagacc gcgccagcca aattgtgata acgcctgcat ccaggtgtcg tccaggcccg
    17761 cgtgaatagt cagcggctcg ccagtaaaag gatgcgtcaa tgacaactga ctggcatgta
    17821 gcattaaccg ctggaggcca aaatgctcag caccgctgcg attctggcgt aaatctccat
    17881 gtttgctatc gccaataatc ggatgacgca aatgggcaag atgtcggcga agctgatgtt
    17941 tgcgtccggt tttcggctcc agttccacca ggccgtagcg cgtggtcggg taacgtccgg
    18001 tcgctaccgg catttctacg gtcgccagac cgcgataatg cgtcactgct ggctgcgggc
    18061 ctttatcttc gcgggcaaat ttatcagcga ttttgtccag ttcttccacc agtggataat
    18121 ccaacaccgc ttcttccatc aaccagccgc gcacaatcgc atggtaacgt ttctggattt
    18181 ggtgctgttc aaactgttgt gccagcagcc gtccggcctc gctggataat cccatcaaca
    18241 acacaccaga agtgggtcgg tccagacgat gaacagtaaa aacatgctgt cctatctggt
    18301 cacgcacggt ttgcatgacc actactttct cgtcgcgatc cagccagcta cggtgaacca
    18361 gccagccgga gggtttattt accgcaacca gccattcatc ctgatagagt atttccagca
    18421 ttagctcgca tcatccgcaa aaagagcatc cagtttttcc aactcagcca gaataagcgc
    18481 gcgttgcgga tggtccgtcg ctagcgccat ttcataatag ggtgcaacag caaaagcgcc
    18541 cggtaacggc tggttgttat ttaacaaatc gtgcattcgc gggatcagcc cccactgcaa
    18601 ccactccagt ggttccatgg tgtccataaa gaacggttgg gtactattaa attgatgcgg
    18661 ctggggttca tcgtttcgcc agtgctgatg ttcacgcagt aacgcttcaa gcgcctgcaa
    18721 ctggagacga acgcggtcat gagtggtcat aaagcaacct caataagaaa aactgccgcg
    18781 aaggatagca ggaataaaaa aagggagcac tgtattcaca gtgctcccgg ttcgtttcgc
    18841 agcattccag ctacttttgt tgctccctgc tcatccttga caacttttcc tctggccttg
    18901 cggccaatcg ttcatcctga actattgctt cctgctcaca ccaccccgat gtgatacttc
    18961 atcctgaagt gtccctggcc ttcctgaccc accgaatcat cctgaccggt tctcattctc
    19021 catcctggag gtgtccttta acgcgtcctg cgtcatcctc ttcgcttcat ccagaagcct
    19081 ttccctgaaa caccatcctg gtgtgtcctg cagaagtgtc atcatcctga tgttcacttc
    19141 gttgtctgac tccctgtcga cgaagataga atcgtctttt tcgcgaagtc ttacaaggcg
    19201 cttacagaca atgccttaag gaaaatttca tatgaaagtt aagaattaac aatcaacatt
    19261 ttgattttaa atgaaaataa cgattcttat ctttttaagt attcgcttat ttcctggcga
    19321 tctcctacag agtctgtaag agatctctca caaccagatg gggtatttgg attacagaag
    19381 aggcttaagt tgattaagga attccgcaag atttgaggct aaatgcatcc gtttacgtgt
    19441 tcccagcgtc tctttgcaca cttcaccgga gagattacac accgagatga cctctaactc
    19501 ttcttccagc gtcgcgataa atagcgtggg cggcagtttt agccttttct gagtcaccag
    19561 atgaccaatc agattttcct gcacccgacg gaagtcgtct tcactccagg tttgcagcaa
    19621 tgtcagtttg atatcgccaa actgcgcgtg catatccccg gcaaactgag tcgtataaaa
    19681 ggtatgaatt gtgggttgta tcacaatatc aaaagcgcgt tcaaccgcgt ttacattttg
    19741 ttccccggta aacggctggg gttgccagta tacggcatct tcggtagttg aaataatgca
    19801 cggtgaagga acaccgtaca gttcctcact taacggccag cttttatgct cttcatgcca
    19861 cgcatcgcag tagcgtgcag taaaatcttt cagggcttgt gcggtcaaat cgtccactaa
    19921 tttctctctt cacttaaacc agatacactt gtcgcttagt ttatctggtt tatgacggtg
    19981 aaacatgtct tcttatgcaa accatcaggc acttgcgggc ctgactcttg gaaaatcaac
    20041 cgattaccgg gatacctatg acgccagcct actgcaaggc gttccacgca gcctgaatcg
    20101 cgacccgctg ggtctgaaag cggataacct gccttttcac ggtacggata tctggacgct
    20161 gtatgaactt tcctggctga atgcgaaagg tttgccgcag gtcgctgtcg gtcatgttga
    20221 acttgattac accagcgtaa atctgattga gtcgaagagt tttaagctct atctcaacag
    20281 ttttaaccag acgcgtttta ataactggga tgaggtgcgc cagacgctgg agcgcgactt
    20341 aagcacttgc gctcagggta agattagcgt ggcgttatat cgtcttgatg aactggaagg
    20401 ccagccgata ggtcatttca atggcacttg cattgatgac caggatatca ctatcgataa
    20461 ctatgaattc actactgact atctggagaa tgccaccagc ggtgaaaaag tagtggaaga
    20521 gacgctcgtc agccacctgc tgaaatcaaa ctgcctgatc acccatcaac cagattgggg
    20581 ttcgatccaa attcagtatc gtggacgcca aattgacaga gaaaaactgc tgcgttacct
    20641 ggtctcattc cgtcatcaca acgagttcca cgaacagtgc gtggaacgca tctttaatga
    20701 cctgttacgc ttctgccagc cagagaaatt gagcgtttac gcacgttata cccgtcgtgg
    20761 cggtctggac attaacccgt ggcgcagtaa tagcgatttt gtcccatcga ccacaagact
    20821 ggttcggcaa taaatttttt ctcaattttg cgtgctggat tcacgcagaa ggttgtgaaa
    20881 ggtcatcagg cagggctatt gtaatcaaag ggaatgacga tattcgtccc ataaggagtt
    20941 tttcttgatt acacatatta gcccgcttgg ctccatggat atgttgtcgc agctggaagt
    21001 ggatatgctt aaacgcaccg ccagcagcga cctctatcaa ctgtttcgca actgttcact
    21061 tgccgtactg aactccggta gtttgaccga taacagcaaa gaattgctgt ctcgttttga
    21121 aaatttcgat attaacgtcc tgcgccgtga acgcggcgta aagctggaac tgattaatcc
    21181 cccggaagag gcttttgtcg atgggcgaat tattcgcgct ttgcaggcca acttgttcgc
    21241 ggttctgcga gacattctct tcgtttacgg gcaaatccat aataccgttc gttttcccaa
    21301 cctgaatctc gacaactccg tccacatcac taacctggtc ttttccatct tgcgtaacgc
    21361 tcgcgcgctg catgtgggtg aagcgccaaa tatggtggtc tgctggggcg gtcactcaat
    21421 taacgaaaac gagtatttgt atgcccgtcg cgtcgggaac cagctgggcc tgcgtgagct
    21481 gaatatctgc accggctgtg gtccaggagc gatggaagcg ccgatgaaag gtgctgcggt
    21541 cggacacgcg cagcagcgtt acaaagacag tcgttttatt ggtatgacag agccgtcgat
    21601 tatcgccgct gaaccgccta acccgctggt caacgaattg atcatcatgc cggatatcga
    21661 aaaacgtctg gaagcgtttg tccgtatcgc tcacggcatc attatcttcc ctggcggtgt
    21721 gggtacggca gaagagttgc tgtatttgct gggaatttta atgaacccgg ccaacaaaga
    21781 tcaggtttta ccattgatcc tcaccggccc gaaagagagc gccgactact tccgcgtact
    21841 ggacgagttt gtcgtacata cgctgggcga aaacgcgcgc cgccattacc gcatcatcat
    21901 tgatgacgcc gctgaagtcg ctcgtcagat gaaaaaatcg atgccgctgg tgaaagaaaa
    21961 tcgccgtgat acaggcgatg cctacagctt taactggtca atgcgcattg cgccagattt
    22021 gcaaatgccg tttgagccgt ctcacgagaa tatggctaat ctgaagcttt acccggatca
    22081 acctgttgaa gtgctggctg ccgacctgcg ccgtgcgttc tccggtattg tggcgggtaa
    22141 cgtaaaagaa gtcggtattc gcgccattga agagtttggt ccttacaaaa tcaacggcga
    22201 taaagagatt atgcgtcgta tggacgacct gctacagggt tttgttgccc agcatcgtat
    22261 gaagttgcca ggctcagcct acatcccttg ctacgaaatc tgcacgtaat ctcccctcc
//
LOCUS       DADMGC010000063        21762 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.063, whole genome shotgun sequence.
ACCESSION   DADMGC010000063 DADMGC010000000
VERSION     DADMGC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.063"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..157
                     /locus_tag="JNZ73_002325"
     CDS             <1..157
                     /locus_tag="JNZ73_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY8732586.1"
                     /translation="LSIEMNKLKQARYSIGIAMSEEKYSGIIGALRGKYINCLVTNSS
                     TAELLLK"
     gene            227..1033
                     /locus_tag="JNZ73_002326"
     CDS             227..1033
                     /locus_tag="JNZ73_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8732587.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            1043..1450
                     /locus_tag="JNZ73_002327"
     CDS             1043..1450
                     /locus_tag="JNZ73_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HAY8732588.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIA
                     VKLEKLASIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            1450..1944
                     /locus_tag="JNZ73_002328"
     CDS             1450..1944
                     /locus_tag="JNZ73_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HAY8732589.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            2010..2807
                     /locus_tag="JNZ73_002329"
     CDS             2010..2807
                     /locus_tag="JNZ73_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY8732590.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            2818..3606
                     /locus_tag="JNZ73_002330"
     CDS             2818..3606
                     /locus_tag="JNZ73_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAY8732591.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            3658..4887
                     /locus_tag="JNZ73_002331"
     CDS             3658..4887
                     /locus_tag="JNZ73_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY8732592.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMELSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            5269..5958
                     /gene="pepE"
                     /locus_tag="JNZ73_002332"
     CDS             5269..5958
                     /gene="pepE"
                     /locus_tag="JNZ73_002332"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HAY8732593.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(6049..7680)
                     /locus_tag="JNZ73_002333"
     CDS             complement(6049..7680)
                     /locus_tag="JNZ73_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAY8732594.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7900..11583)
                     /gene="metH"
                     /locus_tag="JNZ73_002334"
     CDS             complement(7900..11583)
                     /gene="metH"
                     /locus_tag="JNZ73_002334"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAY8732595.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMVDYQMESLSAE
                     INFAAAKLARARADEWTARTPEKPRYVAGVLGPTNRTASISSDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTMPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            11783..12607
                     /gene="iclR"
                     /locus_tag="JNZ73_002335"
     CDS             11783..12607
                     /gene="iclR"
                     /locus_tag="JNZ73_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HAY8732596.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            12924..15108
                     /locus_tag="JNZ73_002336"
                     /pseudo
     CDS             12924..15108
                     /locus_tag="JNZ73_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(15077..16813)
                     /gene="aceK"
                     /locus_tag="JNZ73_002337"
     CDS             complement(15077..16813)
                     /gene="aceK"
                     /locus_tag="JNZ73_002337"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HAY8732597.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPVLMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(16997..18301)
                     /gene="aceA"
                     /locus_tag="JNZ73_002338"
     CDS             complement(16997..18301)
                     /gene="aceA"
                     /locus_tag="JNZ73_002338"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HAY8732598.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGESMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(18331..19932)
                     /gene="aceB"
                     /locus_tag="JNZ73_002339"
     CDS             complement(18331..19932)
                     /gene="aceB"
                     /locus_tag="JNZ73_002339"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HAY8732599.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGCGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(20201..21130)
                     /gene="metA"
                     /locus_tag="JNZ73_002340"
     CDS             complement(20201..21130)
                     /gene="metA"
                     /locus_tag="JNZ73_002340"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAY8732600.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            21287..>21762
                     /locus_tag="JNZ73_002341"
     CDS             21287..>21762
                     /locus_tag="JNZ73_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAY8732601.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGSMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGDKPPLPCLPKLE"
BASE COUNT         5364 a         5567 c         5511 g         5320 t
ORIGIN      
        1 gctctctatc gaaatgaata aattaaagca ggcgcgatat tccattggca ttgccatgag
       61 tgaagaaaaa tacagcggaa ttattggtgc actgcgtgga aaatatatta attgtctggt
      121 aacgaatagc agcacagctg aactattact gaaataaaaa tcttcccgat gtttaccgta
      181 ataaaaatta cggcgggtcc ctattatcta aaatcaggag tctgttatgc aaacgtggtt
      241 aaatttgcag gataaaatca ttattgtcac cggcggcgca tccggtattg gtctggcgat
      301 tgtagaggaa ttattagcac aaggcgcgaa tgtacagatg gtcgatattc acggtggcga
      361 tggtcaatat gaaggtcata aaggctatca gttttggccg accgatattt ctagcacaaa
      421 agaggtaaat catacggtag cggaaattat ccagcgtttt ggtcgcatcg acggtctggt
      481 caataacgcc ggggtcaatt tcccgcgtct gctggtcgat gagaaagcgc ctgccgggca
      541 gtatgaactc aacgaagctg cattcgaaaa aatggtcaat atcaaccaga aaggtgtttt
      601 tctgatgtcg caggcggtgg cgcgacagat ggtcaaacaa catgatggcg tgattgtgaa
      661 tgtttcctca gaaagtgggc tggaaggctc agaaggacaa agctgttacg ccgctaccaa
      721 agccgcgctc aatagcttca cgcgctcctg gtcgaaagag ctgggtaaac acggtatccg
      781 tgtggtcggt atcgcgcccg ggattctgga aaaaaccggg ctacgcacgc cggaatatga
      841 agaggcgctg gcgtggacgc gcaatatcac cgtcgagcag ctgcgtgaag gctataccaa
      901 aaacgccatt cctattgggc gcgccggaag attagcagaa gtagctgatt ttgtttgtta
      961 tctgttgtct gaacgcgcca gctacatcac cggagtaacc actaacattg cgggcggcaa
     1021 aacgcgcggc taaggaggct ttatggtcaa cgccattttt tgtgcccacg gcaaattagc
     1081 ttgcgccatg ctggaatcgg ttcagatggt ttacggcaac gccaatgttg aagccgttga
     1141 atttgtgccc ggtgaaaacg ccggagacat tgcggtaaag ctggaaaagt tagcaagcat
     1201 tcacaatcaa gatgagtggc tgattgccgt ggatttacag tgcggcagcc cgtggaatgc
     1261 ggcagcaatg cttgcgatgc gcaatcctcg tttgcgggta atcagcggcc tttctctgcc
     1321 gctggcgctc gaactggttg ataaccagga cagcatgaat gttgatgaac tgtgtgaaca
     1381 cctgacgcaa atcgcaaagc aaacctgcgt tgtctggaaa caactggcaa cgacagagga
     1441 ggatttctga tgaacattac gctcgcccgc attgatgacc gtctgatcca cggccaggtc
     1501 actaccgtct ggtcgaaagt ggctaacgcc cagcgcatta ttatctgcaa tgacgaagtt
     1561 tataacgatg aagtgcgccg gacattattg cgtcaggctg cgccaccggg aatgaaggtt
     1621 aacgtggtca atattgaaaa agccgtcgcg gtttatcaca acccgcaata tcaggacgaa
     1681 accgtttttt atcttttcac tcgcccacag gatgccttag cgatggttcg ccagggggta
     1741 aaaataggca ccctgaatat tggcggcatg gcctggcggc ccggtaaaaa acagttaacc
     1801 aaagcggttt cattagatga tgatgatatt aacgcatttc atgagttaaa taatcttggt
     1861 gtcatactgg atttacgcgt cgtagcgtca gatccctcaa tcaatattat cgacaaaata
     1921 aacgaacagt taatcgcaaa ttaaataata gcgcctgtgt acatatgctt taacaggcag
     1981 ggatgacgac acttaaaagg tgacaaatca tggaaataag taccctacaa atcattgcta
     2041 tatttctttt ttcctgtatt gccggaatgg gcagcgtgct ggatgaattt cagactcatc
     2101 gcccgttaat tgcctgtacg gtgattggtt taattctcgg tgatttaaaa accggaataa
     2161 tactcggcgg tacgctggaa ttgatagctc tcggctggat gaacgtcggc gcggcgcaat
     2221 ctccggattc tgcactcgcc agcataatct ccgccattct ggttatcgtt ggtcagcaaa
     2281 gcatcgccac cggaatcgcc atcgcgttgc ctgtggctgc ggcaggccag gtgctgacgg
     2341 tgtttgcccg taccatcacc gtagtgttcc agcacgcggc ggataaagca gcagaagagg
     2401 cgcggtttcg cactctcgat attctgcatg tctccgcgct tggcgtgcag gcgctgcgcg
     2461 tcgccattcc ggcactgatt gtctcactgt tcgtcagcgc cgatatggtc agcaatatgt
     2521 taaacgccat tcccgaattt gtgacccgag gactacagat tgctggcggt tttatcgtgg
     2581 tggtcggtta cgccatggtg ctacgtatga tgggcgtgaa atatttgatg cctttctttt
     2641 tcctcggttt cctcgcaggt ggctacctcg atctcagcct gctggcgttc ggtggcgtcg
     2701 gcgtgatcat ggccctggtc tacatccagc taaatccaca gtggcgtaaa gctgaacctc
     2761 aactccaggc caccgcctcc accgcccttg accaacttga cgattaacgg agccatcatg
     2821 gaacagagaa aaattacacg cagcgatctg gtgagcatgt ttctgcgctc caacctgcaa
     2881 caggcgtcct ttaactttga acgtattcac gggctgggct tttgctacga catgatcccc
     2941 gccatcaagc gactttaccc attaaaagag gatcaggttg cggcgctcag gcgacacctg
     3001 gtgttcttca ataccacgcc agccgtatgt ggcccggtca tcggcgcgga aattgatgac
     3061 ggtaccatca acggcatcaa agtcggtctg atgggaccat tggcaggagt tggcgatcca
     3121 ctggtctggg gaacgctgcg cccgattacc gccgcgctcg gcgcatctct ggcactttcg
     3181 ggcaacattc tcggcccgct gctgttcttc tttattttca acgcggtgcg tctggcgatg
     3241 aagtggtatg gcctacagct cggctttcgc aaaggggtga atatcgtcag cgatatgggc
     3301 gggaatgtgc tgcaaaaact caccgaaggc gcgtcgattc tcggactgtt tgtgatgggc
     3361 gtgctggtga ccaaatggac gtcaatcaac gtaccgttgg tggtttcaca aacgcatgcc
     3421 gccgatggct ccaccgtcac catgaccgtg cagaacattc tcgaccaact ttgccctggt
     3481 ttgctggcgc tcggtctgac gctactaatg gttcgtctgc tcaacaaaaa aattaacccg
     3541 gtatggctga ttttcgccct gtttggctta gggattatcg gcaatgctct gggcttcctg
     3601 tcctgattct tcgccccggc acgactgccg aggccatcgc tcaacatgag gtggtttatg
     3661 aaaacaacag ctctgcgtct ttatggtaaa cgtgatttac gcctggaaac ctttgacctt
     3721 cctgaaatgc aggaggatga aatcctcgca acggtggtca ctgacagcct gtgcctctct
     3781 tcctggaaag aggccaatct gggtgaaaac cataaaaaag tacccgacga tgtggcgacc
     3841 aacccaatca tcatcggcca cgaattttgc ggcgatattc tggccgtggg taaaaagtgg
     3901 cagcacaaat tccagccggg tcagcgttat gtgattcagg ccaacctgca actccccgac
     3961 cgcccggatt gccccggcta ctccttcccg tgggtaggcg gcgaagcgac gcatgtggtt
     4021 attcccaacg aggtcatgga acaagattgc ctgctggcat acgacggcga aacctatttt
     4081 gaaggctcac tggttgaacc gctttcctgc gtgattggcg cgttcaacgc caactatcat
     4141 cttcaggaag gtagttataa ccacacgatg gggattcgcc cgcaagggcg cacgctgatc
     4201 ctcggcggca ccggaccaat gggactgttg gcgattgatt atgcgctgca tggaccagtt
     4261 aacccgtcgc tgctcgtcat taccgatacc gacaacgaca aattgagtta tgcgcgcaag
     4321 cactatccat cagaaccgca aacactgatt cattatctca atgccaccga tgcagcattt
     4381 gatacgttaa tggagctgag tggcggtcac ggcttcgatg atattttcgt ctttgtgcct
     4441 aatgaaggac tggtgactct cgcctcttcc ttgctggcga cagatggttg cctgaatttc
     4501 ttcgccggac cgcaggataa acatttcagc gcgccaatta atttctacga tgtgcattat
     4561 gcatttaccc actacgtggg cacgtcaggc ggcaataccg acgacatgcg cgcggcggtc
     4621 aaactgatcg aagagaaaaa agtgcaggcc gcaaaagtgg taacacatat tcttgggctg
     4681 aatgccgcgg gcgaaaccac gcttgaattg cctgccgtcg gcggcggcaa aaagctggtg
     4741 tataccggga aatacctgcc gctgacgtca ctcacgcaga ttcaggatca agcactggcg
     4801 gcgattctgg cgcgtcatca ggggatctgg tccggtgagg cggagcaata cctgctcgcc
     4861 catgcagagg caatttccca tgattaatcg cgctacttaa ctctgcatgt cgctggctgg
     4921 tcgccccagc aacttcgcga ccggatgtgc ggctgattgc tgaagcagca caatttgcac
     4981 gagaaggata aaaaatatca gcgccttaag gggcgctcaa attggtggca aagtacacat
     5041 tgtttatgcc ggatgcggaa tgaagcctta ttcggtctac ataattttga aaatccaata
     5101 tattgcagtt atctggtagg ccaggtaagc gtagcgcatc aggcaatgct gtgtttgtca
     5161 tcagtctcag cgcccggtgc aatacgtgac atcgtgagcc atgtcagtaa gtggttaaat
     5221 tcgcagtggt agcatgcctt ccgtgatttc ttactaaccg gagaagtaat ggaactgctt
     5281 ttattgagta actcgacgct gccgggtaaa gcctggctgg aacatgcact gccgctaatt
     5341 gctgaacagt tgcagggtcg ccgctcagcg gtgtttatcc ctttcgctgg cgtaacgcag
     5401 acctgggatg attacacaga gaaaacggct gcggttctcg ctccgctggg tgtttctgtc
     5461 accggtattc atagcgttgt cgatcccgtt gccgcgattg aaaatgcgga aattgtgatt
     5521 gtcggcggcg gtaatacttt ccagttgctg aaacagtgcc gcgagcgcgg gctgctggct
     5581 ccaattactg acgtggttaa acgtggcgcg ctgtatattg gctggagcgc aggcgctaac
     5641 cttgcttgcc caaccattcg taccaccaac gatatgccga ttgtcgatcc gcaaggtttc
     5701 gatgcgctaa atctgttccc gttgcaaatc aacccgcact tcaccaacgc gctgccggaa
     5761 ggccataaag gtgaaacccg tgagcagcgt attcgcgaac tgctggtcgt cgcgccagaa
     5821 ctgacgatta ttggtctgcc ggaaggtaac tggatcacag tgagtaaagg tcacgctacg
     5881 ctgggtggcc cgaacaccac ttatgtgttt aaggctggtg aagaagcggt tccgctggaa
     5941 gctggtcacc gtttttaagt gaattgccgg atgcatgatc cggcatcttg ttcatagtgc
     6001 ctgatgcgac gcttgacgcg tcttatcagg cctacaggat tcacaatatt aatactcgtc
     6061 ccgtccttca tcttcatccg gctgttccag cacactgtaa gccaccgaac aaaacagcga
     6121 gttcaggcgc tgcatatctc ccagtaagcc taaatgtagc gaactggttt caatgctctg
     6181 cacgttttgc tgatgcaggc gatcgacgtg ggcgtgggaa tagcggcgat taagaatgcg
     6241 aaaacgatgt ttgctgcgac gcaaacgacg agcgctggtg acatcgccag agaagaacac
     6301 cgacattgcc agctttaaat tactgagcaa ttgctcatag agcgcatcca gttccttcaa
     6361 cccatcaagc gaaaatgccc gccgtgctgc cagcgattta tcggcaattt cgctgcccat
     6421 gcgctcgacg atatcggagg cctgttcaag gttgagcgac atttcgatga tctccgccca
     6481 gcggcgcgac tcttcctccg ccaactcttc ttttggcatc cgcgccagat acagcttaat
     6541 ggcggtgtag agaacgttga tatcatctgc cagcttacgc agctctttct cctgccgtgg
     6601 ctcgccgtgc atcactttat tcagcccttc catcatctgt tccatggcgt cgccaatgcg
     6661 cagggtttcg cgcgcggcgt tcgccagagc aagcgtgggc gtgtccagcg cgctgacatc
     6721 cagatgttta ggccgtagct gggtatccag ttccggttca tcgcgaataa tcgttttgca
     6781 aaaccgtgcc atcgggtcaa caaatggcag catgaccagg caacgtacaa ggttgtagaa
     6841 gacgtggaaa tagatcacca gttccgcttt tggcaatgac agcttcccca ttgtctctgc
     6901 cagcaaatgg acaaacggca ggataatcag gctacccacc agcttaaaca gcagactacc
     6961 cagcgcgaca cggcgggctg cggcattggc ggcactgttg ttgagcatcg ccagcaggcc
     7021 ggaaccgagg ttagctccaa tcaccagaca gagcgccacg gggaaggaga taatgcctgc
     7081 agcggtcaga gtcgcggtca gcagtacagc agcaaggctg gagtagctga taatggcgaa
     7141 catcgcgcca atcagcgcat ccagcagaat atcgccggtc agcgaggcaa agatcacctg
     7201 aacgccgttt gcctgggtga tcggcgttac ggcctgcaca atcaactcca gcgccagcaa
     7261 aatcagccca agaccaatac cgacgcggcc cagttgcccg gcgcgtgact gtttgcgtcc
     7321 gaggaaaaaa atcacgccga taaaaataag taacggtgac agccaggata agtcgaaggt
     7381 gagaatacgc gccattagcg ccgtcccgac atctgccccc agcacaatga ccagagccgg
     7441 tgcgagggct accagatcct gagcgacgaa cgaggtcacc agcatggtgg tggcattact
     7501 gctctgtacc agtgcggtaa cgccgatccc cgcgcaaaag gcgagcggct ttttttcgac
     7561 gctccggcta aggacagtac gcaaacgcgc gccgaagacg cgcattacgc cggttcgaac
     7621 aatatgagtc ccccagacca gcagggcgac ggcagaaagc aggtgaagca gcgttaacac
     7681 gagttcaggt tctccttatc gttatatgtt cctgaagcaa ttgaatgacc aaaggaccgg
     7741 aaacacgctg actggcatct ttactgtgcc ttttgacatt cgtttttccg gctaagatta
     7801 ttccagcctg tttccagtat aagtgtttca tattaataaa gagacagggc actcattgag
     7861 cgccctgttt gttgtaagga aaagtgacag atttgtgaat cagtccgcgt cataccccag
     7921 attcggtgcc agccagcgct caacatcgga gacgctcata cctttacggc gggcatagtc
     7981 ttcaacctga tcgcgctgaa tttgcgccac cgcgtagtac ttgctgtccg gatgactgaa
     8041 ataccaaccc gaaaccgatg caccaggcca catagcgaaa gattctgtga gtttcatgcc
     8101 agtatgtttt tccacttcca gcagctccca gatggtggct ttttccgtat gttccgggca
     8161 ggccggatag cccggtgccg gacggatgcc ctggtagttt tcgcggatca gttcttcgtt
     8221 gctgaggttc tcgttcggcg catagcccca gtagacttta cgcacacgct catggagata
     8281 ctccgcaaag gcttcggcta aacggtcggc aagcgctttc accatgattt tgttgtaatc
     8341 atcgtgctgc gcttcaaagg catcagccag tgcgtcctct tccagcccgc cagtcacggc
     8401 aaatgcgccg atgtaatctg ctttaccaga aagcttcggc gcaacgaagt cagcgagaca
     8461 gtagttagcg aatcccgttt tttcggtctg ttgacgcaga tggtggctga cgttgatcac
     8521 atgggtgcgc gtttcgtcac ggtagatttc aatgtcatcg cccacacggt ttgccgggaa
     8581 caggcccacc acgccacgcg gattcagcgt tttctcggcg cttaatttat ccagcatgtc
     8641 gttggcgtct ttaaacagcc gctgcgcctc aacgcccacc acttcatctt ccagaatgcg
     8701 cggatacttc ccggccagcg accaggtcat aaagaacggt gtccagtcga tgtaattacg
     8761 cagcgtttcg atgctggctt ccacttcctg cacgccgaga cggtgcgcca ccggcggcgt
     8821 gtaagcctgc cagtcaaatg cgaaatcgtt atcgcgcgcc gcttccagcg tgaccggtgg
     8881 tgtgcgtggt ttcttgcgcc cgtgctgaat acgcacggtt tcgtactcct tgcaggtacg
     8941 agcgacaaaa tcatcacgct gagtatcgga aagcagcgcc gctaccacac caacggtgcg
     9001 cgaggcattc tgcacataca ccgtagggcc gctgtagttc tgctcgattt tcaccgccgt
     9061 gtgcgctttt gaggtggtcg cgccgccaat cagtaacgga atggtgaagc cctgacgctc
     9121 catctctttc gccacgttaa ccatctcgtc cagcgacggc gtgataagcc ccgaaaggcc
     9181 aatcaaatca gcattcactt ctttggcgat acggagaatt ttttccgcag gcaccataac
     9241 gccgagatcg acaatttcgt agttgttaca ttgcagcacc acaccaacga tatttttacc
     9301 gatgtcgtgg acatcgcctt tcacggtggc gatcaccatc ttgccgttgg ttttaccctg
     9361 ctctttgctg gcttcaataa acggttcgag atacgccact gcctgtttca tgacgcgcgc
     9421 cgatttgacc acctgtggca ggaacatttt gccttcgcca aacagatcgc cgaccacgtt
     9481 catgccgtcc atcaacgggc cttcaatcac ttcaatcggg cgcgtagcct gctggcgggc
     9541 ttcttcagta tcctgctcga taaactcggt tatgcctttg accagcgagt attccagacg
     9601 tttattcacg tcccacgagc gccactccgc ttgctgggcg ttagcagtgt cgtccgcttt
     9661 actgccgcga tatttctcgg caagctccag taaacgctca gtgccatcgt cacgacgatt
     9721 aagaatcaca tcttccaccg cgtcgcgcag ttcagcgggt aggtcgtcgt aaatcgccag
     9781 ttgcccggcg ttgacgatcc ccatatccat gccattgcga atagcgtagt agaggaacac
     9841 tgcgtgaatg gcttcgcgca ccggatcgtt gccacggaat gagaaagaaa cgttagatac
     9901 gccgccggaa atcagcgcgt gcggcagttc gcgtttgatg tcttcacacg cgccgataaa
     9961 gtcctgcgcg tagttgttgt gctcttcaat accggtggcg accgcgaaga tgttcgggtc
    10021 gaagatgata tcttctggcg ggaagccaac ctcttcggtg aggattttgt acgcccgacg
    10081 gcaaatctcg attttccgtg cgcgagtatc ggcctgtccc tgttcgtcaa aggccatcac
    10141 caccactgcc gcaccgtagc gacgcaacaa tttcgcgtga tggataaagg cgtcgacgcc
    10201 ctctttcatc gagatagagt taacaatgcc tttgccctgg atacacttca gacctttttc
    10261 aatgacatcc cattttgagg agtcgatcat aatcggcacg cgagcgatat ccggttcacc
    10321 ggcaatcaga ttgagaaaac gcaccatcgc cgcttcggca tcgagcatcc cttcatccat
    10381 gttgatatcg ataatctgcg cgccgttttc cacctgctga cgcgccacat ccagcgcctc
    10441 gctgtatttc tcttctttga tcaggcgctt gaacttagca gagccggtga cgttggtgcg
    10501 ttcacccacg ttcacaaaca ggctatcatc gccaatgttc agcggctcca ggccagacaa
    10561 acggcaggct acgggaattt ccggcagttt gcgcggcgct aatccttcta ctgcacgact
    10621 catcgctgca atatgttgcg gcatggtccc gcagcaacca ccgacgatat tgagaaagcc
    10681 cgcttgcgcc cattcacgta tctgttttgc catcgtgtcg gcgtcgagat cgtattcacc
    10741 aaaggcgtta ggcagcccgg cgttcgggtg ggcggtgacg tagcattccg caatccgtga
    10801 tagttcctgc acgtactggc gcagttcatc gggccccagc gcacagttca ggccaaaggt
    10861 cagcgcttcg gcgtggcgca atgagttgta aaatgcttcg gtggtctgcc cggagagcgt
    10921 gcgcccggag gcgtcggtga tggtgccgga gatcataatc ggcagctcaa cgcccagcgc
    10981 ttcaaactcc gttttcaccg caaagaccgc cgctttggcg ttaagggtgt cgaaaacggt
    11041 ttcaatcagg atcagatccg caccaccttc caccagcgct ttggtggatt cgcgataggc
    11101 cgccaccagc tggtcaaaag tgatgttgcg gaatgccgga tcgttgacgt ccgaagaaat
    11161 agacgccgtg cggttggttg ggccgagaac accggcaacg tagcgcggtt tctccggcgt
    11221 gcgcgcggtc cactcgtcag cacgggcgcg cgccagtttc gccgccgcaa agttgatttc
    11281 cgccgacagg gattccatct ggtaatccac catcgcaatg gtcgtggagt tgaaggtgtt
    11341 ggtttcgatg atatccgcgc ccgcttcaaa gtaggcgttg tggatagcgg cgatcacttc
    11401 cggtttactg agtaccagca ggtcgttgtt gcctttgagg tcgcatggcc agtcggcaaa
    11461 gcgttcacca cgaaaatcgg cttcgttcag tcgatagctc tggatcatgg tgcccatacc
    11521 gccgtccaac accagaatac gttcatttaa ctgcgcacgc agttgttcca ctttgctgct
    11581 cacacttgct cccgacacgc tcaacccaga tttaataaaa attcaacaaa ccatactggc
    11641 ataaacgcat ctgtggtaaa agcgaccacc acgcaacatg agatttgttc aacattaact
    11701 catcggatca gttcagcaac tattgcatta gctaacaata aaaatgaaaa taatttccat
    11761 gatacagaaa aaggaggctg tcatggtcgc ccccattccc gcgaaacgcg gcagaaaacc
    11821 cgccgttgcc accgcaccag cgactggaca ggttcagtct ttaacgcgtg gcctgaaatt
    11881 actggagtgg attgccgaat ccaatggcag cgtggcactc acagagctgg cgcaacaagc
    11941 cgggttaccc aattccacga cccaccgcct gctaaccacg atgcaacagc agggtttcgt
    12001 gcgtcaggtc ggcgaactgg gacactgggc aatcggcgca catgccttta tggtcggcag
    12061 cagctttctc cagagccgta atttgttagc aattgttcac cctatcctgc gcaatctaat
    12121 ggaagagtct ggcgaaacgg tcaatatggc ggtgcttgat caaagcgatc acgaagcgat
    12181 tattatcgac caggtacagt gtacccacct gatgcgaatg tccgcgccta tcggcggtaa
    12241 attgccgatg cacgcttccg gtgcgggtaa agccttttta gcccaactga gcgaagaaca
    12301 ggtgactaag ctgctgcacc gcaaagggtt acatgcctat acccacgcaa ctctggtgtc
    12361 tcctgtgcat ttaaaagaag atctcgccca aacgcgcaaa cggggttatt catttgacga
    12421 tgaggaacat gcgctggggc tacgttgcct tgcagcgtgt attttcgatg agcaccgcga
    12481 accgtttgcc gcaatttcta tttccggacc gatttcacgt attaccgatg atcgcgtgac
    12541 cgagtttggc gcgatggtga ttaaagcggc gaaggaagtg acgctggcgt acggtggaat
    12601 gcgctgactt tttctggcgg gcagaggcaa tattctgccc atcatacctg agtggcaata
    12661 gaataagggt gtctgttaat cgcattgacg ccaaaataac ttaatgtcat acacttcaca
    12721 cacttttatt aactcagcat tatttttaaa catcaaacca cttaattatt aacaaaatcg
    12781 caagatctca ttccaccgaa acttttacgg ctgcattatg agattgagcg atttatgata
    12841 tttttttagt tgtaatcata ttaaattttt tcactgttct gatagttaaa attcaagaca
    12901 tcaataaaca atgagatatt taaatgatta ctcgtattcc tcgtagttct ttctctgcaa
    12961 atattaataa tacagcccag acaaatgaac accaaaccct gagtgaattg ttttataaag
    13021 aactcgagga taaattttct ggcaaggagc tggcgacgcc tctattaaaa agcttctcag
    13081 agaactgtag acaaaatgtt cgtcatatct ttagcaacaa ggattttgtc attaaatttt
    13141 ccacgtccgt cttacaagct gataagaaag aaattacgat aattaataaa aacgaaaaca
    13201 cgacactcac tcaaaccatt gctacaatat ttgaagaata cctaattgaa attttacctc
    13261 aacgctcaga cactcttgat aaacaagaat taaacctaaa atcagataga aaagaaaaag
    13321 aattcccaag aattaaactt aatggtcaat gttattttcc ggggcgcccc aaaaccgtat
    13381 agtataccga cacattgctg cacaatatat taatgatatt tatcagaatg ttgattacaa
    13441 accccatcaa gatgattact cttcagctga aaaatttctc actcacttca acaaaaaatg
    13501 caaaaaccag actttggcgt tgatttccag ccgtcctgag gggcgttgcg ttgctgcctg
    13561 cggtgatttc gggctagtta tgaaagcata ttttgacaag atggaatcaa atggcatcag
    13621 tgttatggca gccatattac tggtggataa ccatgctttg acggtccggc taagaataaa
    13681 gaacacaact gaaggatgta cccattacgt gatttcggtt tatgatccta atgtaactaa
    13741 cgataaaata agaattatga gcgaaagcaa agaggatatt aaacactatt ctctgatgga
    13801 ttttatgaat gtagattata gcctcctgaa atggtcaaat gatcatgtta ttaaccaatc
    13861 tgttgcaata attccagcac ttccgaaaga acagctattg atgttaaaag gaactgtgga
    13921 tgaaataacc cctccattat caccagcaac gatgaatttg ctaatggcaa ttggtcagaa
    13981 tcaccaactt acgcaactga tgattcagct ccagaaaatg ccagaactac atagaacaga
    14041 aatgttgact gcctataata gtattaacct ccccggttta tatttggcta taaattatgg
    14101 caatgcggat atcgttgaga ctattttcaa ttcattgtca gaaacaggat atgaagggtt
    14161 actctcgaaa aaaaatctca tgcatattct ggaggcaaaa gataaaaatg gtttttctgg
    14221 attattttta gcgatatcac gtaaggataa aaatgttgta acctcgattc tgaacgtctt
    14281 acctaaactg gccgcaacac atcatttaga taatgaacag gtgtataaat tcctgagtgc
    14341 caaaaataga acgtccagcc atgttttata ccatgttatg gcgaatggtg atgccgacat
    14401 gctgaaaatt gttttggtcg cgttaccttt gttaattcgc acatgtcatt tgactaaaga
    14461 acaggtactc gatctcctga aggcaaagga tttttatggt tgcccaagac tatacctggc
    14521 gatgcaaaat ggacatagct atatcgtgaa agttattctc gaagcattgc cctgtctggc
    14581 ccaggaaatt aacatttcag cttccgatat tgtagatctt ctgaccgcta aaagtcttgc
    14641 gcgcgacacg ggtttgttta tggccatgca gcgcggacat atgaacgtta ttaatactat
    14701 ttttaacgca ttacccactc tttttaatac gtttaaattc gataaaaaaa tatgaagccc
    14761 ctcctcctgg caaataattc taatgaatat cccggtttgt tttcagcgat acagcataaa
    14821 caacaaaacg ttgtagagac ggtttatctt gctttatctg accatgcacg cctgtttgga
    14881 tttaccgctg aagatattat ggatttttgg caacacaaag cgccacaaaa atactctgcc
    14941 tttgagttgg cttttgaatt tggtcaccgg gttattgctg aattaatcct taatacatta
    15001 aataagatgg ctgaaagctt tggctttacg gataaccctc gatacattgc ggagaaaaat
    15061 tatatggaag ctttactcaa aaaagcatct ccccataccg tacgctaaat ctttgcctgc
    15121 gccgatacgc ataaacatct tccacatgcc cttcacgtat gcggttttgt agtgcgcgcc
    15181 agtaatcagc gcggaacagg tcggcgtgca tctcttcaaa cagcggacca atacgcgggt
    15241 cggcgcatag ccagtggcga aactcttccg ggaaaacatc gcccggcgag acgctgtacc
    15301 acggttcgct ggcaagttcg tcttccggat agcgcggcgg cgggatgtcg cggaaattca
    15361 cttccgtcat gtagcaaatt tcatcgtaat cataaaaaac cacacgcccg tgacgggtga
    15421 caccgaagtt tttaaacagc atgtcgccag ggaaaatgtt agcagcggca agctggcgaa
    15481 tagcgttacc gtattcttca atggcgtcgc gcaactgctg accttccact tgttccagcc
    15541 agatattgag cggcaccatc cgccgctcga tatagagatg gcgaattaca atctgctcac
    15601 caagatcggt gattttttcc gctgcttcct ggagcagtaa ttccattaat accggggaaa
    15661 tatgccgctt ctccagcaca aagttttcaa actcctgggt gtccgccatt cggcccacac
    15721 ggtcgtgctc tttcaccagt tgatagcagg cacgaacgtg agcggcagac atctctttct
    15781 gcggcgcgaa cttgtctttg atgactttga atacccgatc aaagcccggc agcgtaaaca
    15841 ccaacatcac cattccacga atacccggcg cttcaatgaa ctgctcatta cagccctgta
    15901 gataaacgag atattcgcgg tagctttcgg ttttggcgtg cttctggcag ccgatagcca
    15961 tatacaattc agcagtggtt ttacctggca gaatttcccg tagccactcg accagcgctg
    16021 cgggcagcgg tgcataaacc ataaaataag aacgcgcaaa gccaaaaaca atgctcgctt
    16081 cggcggtcgt cgtcaggcag gtatcaataa ataactcgcc gtcgtccgtc tggtggatcg
    16141 gcagtaagaa gggcaacgtg ccggaaggcg tgatcagttt gcccaccagc caggcggctt
    16201 tattgcggta aaacagttcg ttcgccacct gtaaatgact ttccgcgagg ttgtctgtcc
    16261 ccagcgtttc cgtcagatgg cgaatgatgt aatgaatgtc acggctttta ttctgccagc
    16321 gcaggcgcag cggcaggtcg ctgataacgc gcatcagtag agattcccag ccgtgatcgg
    16381 ggtgaaagtc tttcgccagc gggcggggaa tggtacgaaa gcggcgctct ggctgagagc
    16441 taaagataaa aagccgctcg ggagtaagcg agcggtggtc aaataaccga cagtacacgg
    16501 agttaaaaaa gctctccgca atctcgaagc gcgggtaatc cggcaacagc cgcgtgtaat
    16561 gctctttaac gcgcagtaaa aatgccgcgt ccgtactttg gccgttagta atgcagcgca
    16621 gttgctccac gaccagacca acgtgatgat cgtaaagatg gatacggttt ttcatcgcct
    16681 gctggacagc atgccagtcg gcctgttcga aacgctgctg cgccccggaa gtcacttcga
    16741 ggaatcgacc atactgagcg tcgaagcctt gcaaaatggt ttgagcaatc agtaattcca
    16801 ggccacgcgg catttagcgc actcatcagg agcagagaat tgccggatgc ggcgtaaaca
    16861 ccttatccag cctacgttcg gcaacggttg taggcatgat aagacgcgca agcgtcgcat
    16921 caggcatcgt gcaccgattg ccggatgcgg cgtaaatgcc ttatccggcc tacagtcagc
    16981 aacgtttgta ggttgcttag aactgcgatt cttcagtgga gccagtcagc gcggtgactg
    17041 aagacgtgcc gccctgaata atagtcgtca ctttatcgaa gtaacctgta cccacttcct
    17101 gctggtgaga tacgaaggta tagccatctt tcgcggcggc aaattccggc tgctgcactt
    17161 tctcaacata gtgcttcata ctttcgccct gcgcataagc gtttgccagg tcgaacatgt
    17221 taaaccacat gctgtggata cctgccaggg tgatgaactg gaacttgtag cccatatccg
    17281 acaactgctg ctggaagctg gcaatagttt tgtcgtcgag gtttttctgc cagttgaacg
    17341 acggcgagca gttataagcc agcagtttgc ccggatattt cgcgtggata gcttgtgcaa
    17401 agcgacgcgc cagttccaga tccggcgtgg aggtttcaca ccagaccagg tcagcatatg
    17461 gcgcatacgc caggccacgg ctgatcgctt gctcaatgcc cgcatgagta cggaagaagc
    17521 cttcactggt acgctcgccg gtaataaatt cgctgtcata cgggtcgcaa tctgaggtga
    17581 tcagatccgc cgcatcagca tcggtacgtg caaccagcag ggttggaacg cccgtcacgt
    17641 cagctgccag acgcgccgcg accagtttct gaatagcttc ctgagttggc actaaaactt
    17701 tgccgcccat gtgaccgcat ttcttcactg acgccagctg atcttcgaag tgaactgccg
    17761 cagcaccggc ttcaatcatc gctttcatca gttcaaaggc attcaggaca ccgccaaaac
    17821 cggcttccgc atcggcaacg atcggcagga agtaatcgac atagcgcgga tcgcccggct
    17881 caatgcccac ggaccattgg atctgatcgg cacgacggaa ggtgttgttg atccgctcca
    17941 ccacagctgg caccgagttt gccggataga gcgactgatc cggatacatg ctggccgcca
    18001 ggttagcgtc cgccgctacc tgccatcccg acagatagac tgcttcaata cccgctttcg
    18061 cctgttgcag cgcctgaccg ccagtcagtg caccgaggct gttgatgtag ccttttttcg
    18121 actcaccgtg cagcagacgc cacattttcg ctgcgcccag ttgcgccagc gtgcattcag
    18181 gattgactga accgcgtaat ttcaccacat cttccgcact gtatgggcga gtaatgcctt
    18241 cccaacgcgg ttgagtccac tctttctgta attcttcaat ttgttgtgta cgggttttca
    18301 tgtgcagatg ctccatagtt atgtggtggt ttacgctaac aggcggtagc ctggcagggt
    18361 caggaaatca attaactcat cggaagtggt gatctgttcc atcaagcgtg cggcatcgtc
    18421 aaaacggccc tgggagaaac gttcttcgcc cagttcgctg gcaatgactt tcatctcttc
    18481 gcccagcatc tggcggaaca aggctttggt caccggtttg ccattgctca acgttttttg
    18541 atgatggatc cactgccaga tcgaggtacg ggaaatttca gccgtcgccg catcttccat
    18601 caggccataa atcggcacgc agccgttgcc ggagatccac gcttcgatgt actgcacagc
    18661 cacgcgaatg ttggcgcgca taccttcttc ggtgcgttca ccatcacaag gtgccagcag
    18721 ctgatcggca gtaatcggcg cgtcttgttc gcgcaatact tcaagctgat ttttacggga
    18781 gccgagaata tcgttgaata ccgccattgc cgtgtccgca aggcctgggt gagcgatcca
    18841 tgtgccatcg tgaccgttat tggcttccag cgctttatcc gcttttactt tgttgagcac
    18901 ctggttatta cgctcttcat ctttgctcgg aataaacgct gccatgccgc ccatcgcaaa
    18961 agcaccgcgt ttatggcagg ttttaatcag caggcgtgag taagcattca ggaatggttt
    19021 atccatcgtc acggcctgtc tgtctggcag gacacgatcg ggatagtttt tcaacgtttt
    19081 gatatagctg aagatgtaat cccagcgacc gcagttcaga ccaacaatat ggtcgcgcag
    19141 cgcgtgaagg atttcgtcca tctggaacac cgcgggcagc gtttcaatca gcaacgtcgc
    19201 cttgatggtg ccgcgcggca gattaaagcg atcttctgca tagctgaaga cttcgctcca
    19261 ccaggccgct tcctgccagg actgggtttt cggcagatag aaatagggac cgcagccctt
    19321 tgccaacagt gcctgatagt tgtggaagaa atagagcgca aaatcaaaca ggctaccggg
    19381 gattgcctca ccacgccagg tgacatgttt ttccggcaag tgcagaccgc gtacccgaca
    19441 aatcaaaacc gctggattgg gcttgagctg ataaattttg cctgcttcat tggtatagct
    19501 gatggtaccg ttaaccgcat cgcgcaggtt aatttgcccg tcgatcactt tgttccagtc
    19561 tggtgccagt gaatcttcga aatcggccat aaagactttc acattggcgt tcagtgcgtt
    19621 gatcaccatc ttgcgctcta ccgggccagt tatctctacg cggcggtctt ctaagtccgc
    19681 aggaatcccg cgaattttcc aatcagcatc gcgaatggaa gccgtttccg aaataaaatc
    19741 aggcaacgtt ccgttatcaa tatcttgctg ctgctgaatg cgcgctgcca gaagtttatt
    19801 gcgttgtggc gtaaaatgcg tcaccagctc agtcagaaat tctaccgctt cggcagtaag
    19861 aatttgcttc tcctgctcgc catacggcct tgtgaaagcc agttcatcgg ttgttgttgc
    19921 ctgttcagtc atcgtgcagc tcctcgtcat ggatccgaaa acttcccctc tgtgaacgaa
    19981 ggaatgttgt gcgcttttcg ttcagcacaa ctaagactac tcatttaaaa tgcaaaaaca
    20041 aaaacaattt ccattttaat taaaaatact tgataactta ttgataacaa tatgaataaa
    20101 atttattcac tttctgaggt gcgtttcgga aataaaaaag gcacccgaag gtgcctgagg
    20161 taaggtgctg aatcgcttaa cgatcgacta tcacagaaga ttaatccagc gttggattca
    20221 tgtgccgtag atcgtatggc gtgatctgat agacgtaata gttgagccag ttggtaaaca
    20281 gcaaattacc gtgactacgc cagctcgctc gcggtttgtt ttgcggatcg ttgtgcggga
    20341 aatagttata cggtacatcc gggtctagtc cggcttccac atcgcggaaa tattcctgcg
    20401 ccagcgtttg cgcatcatat tcgggatggc ccgtcacaaa ggcaatgcgc ttatctttgc
    20461 tggcaaacag atatgcatcc ccttcttccg tctctgccag aatttccaga tcggtgtaat
    20521 cacgaatcaa cgctgccgga aagtcagcat agcgcgaatg cggtgccagg aatgaatcat
    20581 caaagccacg cgtcagaagc gcatgaggat ggagaatatg atgctcgtaa acgccagaga
    20641 gtttgtcggt gcgagtttgc ttaggaatgc cgtagaggat attgagcgcg gcctgtaccg
    20701 cccagcagac aaacagcgtc gaggtgacgt gatctttcga ccactccagc acctgtttga
    20761 tctgcggcca gtaagcgaca tcattaaact ccaccaggcc cagcggcgca ccggttacaa
    20821 tcagaccgtc aaagttctgt tcctgaatat cttcaaagtt acagtagaag ttgttcagat
    20881 gctctgcggg cgtgttgcgc gattcacggg aatcgatgcg caacagctga atatcgacct
    20941 gcaaaggtga gtttgaaagc aggcgcagaa actgattttc agtttcaatc ttcttcggca
    21001 tcaggttaag gatcagaacc ttaagtggac gaatttcctg accagacgca cgagaagttg
    21061 tcatcacaaa cacgttttct tcacgcaaga aattgacggc gggtagctcg tccggcacac
    21121 gaatcggcat aacctgatta cctcactaca tacgcttata cgtttagaca tccagatagc
    21181 tgaagataac cagaaaattt gccaatgtcg agcctgcatg ttgaaggtga gaaagtttca
    21241 aggaaaaggc tacgttagaa tataagaatg acgaaaagga gagaaaatgg ttattagtat
    21301 tcgccgctca cggcatgagg aaggggagga actcgttgcg atttggtgtc gatctgtcga
    21361 tgccacccac gattttttat cagcagagta tcgggccgag ctggaagagc tggtgcgttc
    21421 ctttctgccg gaagcaccgt tgtgggtcgc ggttaatgaa cgggagcagc cggttgggtc
    21481 tatgttgtta agtgggcagc atatggatgc gctgtttatc gatcctgatg tgtgcggctg
    21541 cggcgtgggt cggatgctgg tggagcatgc gctctcgatg gcaccggaac tgacaaccaa
    21601 cgttaatgag caaaatgagc aggcggttgg gttctataag aaggtgggtt ttaaggttac
    21661 gggacgctct gaggtggacg atttggggaa accgtatccg ttgctgaatc tggcgtatgt
    21721 gggggataag ccgcccctgc cgtgcctgcc caagctggag aa
//
LOCUS       DADMGC010000064        21516 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.064, whole genome shotgun sequence.
ACCESSION   DADMGC010000064 DADMGC010000000
VERSION     DADMGC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.064"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="JNZ73_002342"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="JNZ73_002342"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HAY8732602.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="JNZ73_002343"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="JNZ73_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAY8732603.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLHD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="JNZ73_002344"
     CDS             complement(2649..3200)
                     /locus_tag="JNZ73_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HAY8732604.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="JNZ73_002345"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="JNZ73_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HAY8732605.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="JNZ73_002346"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="JNZ73_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HAY8732606.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="JNZ73_002347"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="JNZ73_002347"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HAY8732607.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="JNZ73_002348"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="JNZ73_002348"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAY8732608.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="JNZ73_002349"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="JNZ73_002349"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAY8732609.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHTESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVTWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="JNZ73_002350"
                     /pseudo
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="JNZ73_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="JNZ73_002351"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="JNZ73_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HAY8732610.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="JNZ73_002352"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="JNZ73_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HAY8732611.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="JNZ73_002353"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="JNZ73_002353"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAY8732612.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="JNZ73_002354"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="JNZ73_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HAY8732613.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="JNZ73_002355"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="JNZ73_002355"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HAY8732614.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="JNZ73_002356"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="JNZ73_002356"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAY8732615.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKNKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSGNTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="JNZ73_002357"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="JNZ73_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HAY8732616.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="JNZ73_002358"
     CDS             complement(18174..18569)
                     /locus_tag="JNZ73_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY8732617.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="JNZ73_002359"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="JNZ73_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8732618.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="JNZ73_002360"
     CDS             19485..20078
                     /locus_tag="JNZ73_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY8732619.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..20675
                     /locus_tag="JNZ73_002361"
     CDS             20223..20675
                     /locus_tag="JNZ73_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY8732620.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20798..>21516
                     /locus_tag="JNZ73_002362"
     CDS             20798..>21516
                     /locus_tag="JNZ73_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY8732621.1"
                     /translation="MSKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKAL"
BASE COUNT         5202 a         5329 c         5662 g         5323 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccaccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcacgaccg
     1501 ctgggtgcta gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gttctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggctcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaga catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtagag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgccgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 ctttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaag ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gacgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtagt tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gattaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgtcac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agccaacgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgt atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tcgagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg ggaggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gctatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgaccttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acacaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctccgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg atcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc caggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tgccgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg ttcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac gcctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggcaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 accgccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttaccggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacaggttt tgtaatggtt actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagc ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcggggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaaacattg ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag cacgatatcg
    20341 aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc
    20401 gtgcggagta ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca
    20461 tgaccttcag cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca
    20521 tcgcattttt gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg
    20581 ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg
    20641 ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgccgta aataactcca
    20701 ggtttacggc gagtttgtga aaagagcgtt ttttgatatt tttttgtgag taaaatttgt
    20761 aatgcttaga cgttcttatt aactcaagga gttcgtcatg agcaaaatat cagatttgaa
    20821 ttattctcaa cacattacat tagccgacaa ttttaaacaa aaaaatgaag ccttagatac
    20881 ctggtatgtg ggaatgaatg attttgcccg aattgccggg gggcagaata gcagaagcaa
    20941 tattctttct cctagagcat ttttggagtt tttggcaaag atatttaccc taggttatgt
    21001 ggattttagc aaacgctcca acgaagcggg tagaaatatg atggctcata ttgagttctc
    21061 atcttattct aaagatactg atggcaatga aaaaatgaag ttttacatga ataatcctga
    21121 aggggagcga gcggatttat ctaaggtgaa gatagaaatt acacttgcct cagcttctac
    21181 taaagggatt cgtgaaggac atacagtcat tatatttaaa caatctgatg gttcgactaa
    21241 tcgttatgaa gggaagtctt ttgaaagaaa agatgatagt tcattacacc tgattactaa
    21301 caaggttctg gcgtgttacc aacgcgaagc taacaagaaa atagcgcggc tattaaataa
    21361 tcatcagaag ttaaataata tacaggagtt aaatgattcg caggagttaa ataattcaca
    21421 gaagttaaat aattcgcagg agttaaataa ctcgcaggtg agttgtaaag gttcagttga
    21481 ttctacgatt acggatttat tagaaaaagc attgaa
//
LOCUS       DADMGC010000065        21505 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.065, whole genome shotgun sequence.
ACCESSION   DADMGC010000065 DADMGC010000000
VERSION     DADMGC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.065"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="JNZ73_002363"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="JNZ73_002363"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAY8732622.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="JNZ73_002364"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="JNZ73_002364"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAY8732623.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="JNZ73_002365"
     CDS             complement(3121..3420)
                     /locus_tag="JNZ73_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732624.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /locus_tag="JNZ73_002366"
     CDS             3595..4590
                     /locus_tag="JNZ73_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8732625.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="JNZ73_002367"
     CDS             complement(4632..5069)
                     /locus_tag="JNZ73_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HAY8732626.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="JNZ73_002368"
     CDS             complement(5115..5456)
                     /locus_tag="JNZ73_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732627.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5726..7078)
                     /gene="xylB"
                     /locus_tag="JNZ73_002369"
     CDS             complement(5726..7078)
                     /gene="xylB"
                     /locus_tag="JNZ73_002369"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAY8732628.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGMWGQHWAQQGWRRLRRIQRNRSLSCCRSYR
                     "
     gene            complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="JNZ73_002370"
                     /pseudo
     CDS             complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="JNZ73_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8838..9830
                     /gene="xylF"
                     /locus_tag="JNZ73_002371"
     CDS             8838..9830
                     /gene="xylF"
                     /locus_tag="JNZ73_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8732629.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKEREL"
     gene            9908..11449
                     /gene="xylG"
                     /locus_tag="JNZ73_002372"
     CDS             9908..11449
                     /gene="xylG"
                     /locus_tag="JNZ73_002372"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HAY8732630.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11427..12608
                     /gene="xylH"
                     /locus_tag="JNZ73_002373"
     CDS             11427..12608
                     /gene="xylH"
                     /locus_tag="JNZ73_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HAY8732631.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12686..13864
                     /locus_tag="JNZ73_002374"
                     /pseudo
     CDS             12686..13864
                     /locus_tag="JNZ73_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
     gene            complement(13972..14796)
                     /locus_tag="JNZ73_002375"
     CDS             complement(13972..14796)
                     /locus_tag="JNZ73_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HAY8732632.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15116..17146
                     /gene="malS"
                     /locus_tag="JNZ73_002376"
                     /pseudo
     CDS             15116..17146
                     /gene="malS"
                     /locus_tag="JNZ73_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            17324..18577
                     /gene="avtA"
                     /locus_tag="JNZ73_002377"
     CDS             17324..18577
                     /gene="avtA"
                     /locus_tag="JNZ73_002377"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HAY8732633.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGETELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18728..19201)
                     /locus_tag="JNZ73_002378"
     CDS             complement(18728..19201)
                     /locus_tag="JNZ73_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY8732634.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="JNZ73_002379"
     CDS             complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="JNZ73_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HAY8732635.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTHNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGALT"
     gene            20352..21350
                     /gene="yiaK"
                     /locus_tag="JNZ73_002380"
     CDS             20352..21350
                     /gene="yiaK"
                     /locus_tag="JNZ73_002380"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HAY8732636.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21362..>21505
                     /locus_tag="JNZ73_002381"
     CDS             21362..>21505
                     /locus_tag="JNZ73_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HAY8732637.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
BASE COUNT         5405 a         5487 c         5281 g         5332 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaacctt tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 cccccgtacg gtaatcgagc tgctgaccgc tgatatccgc cagcatctga cgccagtact
     5881 cactacgcgc cccgccacca atcaacgtaa cactttgcgg tttaatgccg caggcatgca
     5941 cgacatccat gccatctgcc agcgcataac ccacgccttc cagcactgct cgcgccagtt
     6001 cattggggcc atgttgatga gtcaaaccaa agaaaacccc cttcgcctgg ggattattgt
     6061 gtggtgtacg ctcgccggaa agataaggca gaaaccaaac tggctcggca ctttcatcag
     6121 cctgttgagc tgcagcgatt aaagctggga cattgcacag gccggttaat ttcgcggccc
     6181 aatccagaca cgatgctgca ctcagcatta cagacattaa atgccaacgc tgcggtagcg
     6241 catggcaaaa gctgtgtaca gcgctttctg gcttgcttaa gaacccttcg ctgacagcaa
     6301 aatagacccc cgacgtcccc agcgataaca ttgcctgatt agcatcaacc attcccacac
     6361 caactgcacc agctgcattg tcgccaccgc ctgcgacaac tggcaccgtc gccatacccc
     6421 acgctttcgc aacttcaggt aacaaagcac cagtaatttc gctgccttcg tataatgcgg
     6481 gcatctggtc acgagataag tcgcaagcct gcagcatgac gtcactccag tcacgctttg
     6541 cgacatccag ccacatggtg ccagctgcgt cagacatatc gctggcaaac tcccccgtca
     6601 tacgcagacg caagtaatct ttcggtaata atactttgtc gatttgacgg aatatctccg
     6661 gctcatgccg ctgaacccat agcaatttag gcgcagtaaa tccgggcatc atcaggttgc
     6721 cggtaatcac tcgtgattgc ggaactctcg cttccagcaa agtgcactct tgcgcacagc
     6781 gcccgtcgtt ccacaaaatg gcagggcgca atacccgttg ttgagcatcc agtaaggttg
     6841 ctccatgcat ctggccggca atacccaatg ctttaacgtc ctgcagagaa tgctgatcgc
     6901 ccagagcttt cattgcgcga tcagttgcct gccaccactg ttccgggtct tgttccgacc
     6961 aaagtggatg cgggcgcgaa acggtcagct tttccgtttg cgaagcaacc acctcaccct
     7021 gctcgttgag caaaataact tttacgcccg aggtgccaag atctatcccg atatacatat
     7081 cgatcgttcc ttaaaaaaat gcccggtatc gctaccgata accgggccaa cggactgcac
     7141 agttagccgt tatttgtcga acagataatg atttaccaga ttttccagtt gctcctggcg
     7201 accactctga tgcaccggag acaaattatg ttcctgagca tatttggcta aatctgccag
     7261 tgacatttgg cctttcagga tttgctggcc caattcgcta ttccagccgg aataacgctg
     7321 cgcgatgcgt ttatccagct cgccatcttc aatcatgcgc gctgcaattt tcagcgccag
     7381 tgccatcgta tccatcgcgc cgatatgacc gtaaaacaga tcatatttat cagtactttg
     7441 acgacgtact ttggcatcga agttcagacc accggtggtg aaaccgcctg ctttgagaat
     7501 ttcatacatc accagcgcat tctcttccac actgttcggg aactggtcgg tgtcccagcc
     7561 cagttgcgca tcgccacggt tggcgtcgac agaaccgaac aggccaagcg caatggcggt
     7621 ggctatttca tgatggaaag agtgacctgc cagcgtcgcg tggttagctt caatgttcag
     7681 tttaatctct ttttccagac caaactgttt caggaagcca tagaccgtcg cggcatcgta
     7741 atcatattga tgtttagtcg gttcttgcgg tttcggttcg ataagcaacg tgccctagaa
     7801 gccgatttta tgtttatgct caaccaccat ctgcataaag cggcccagtt gttcacgctc
     7861 ctgacgcaag tcggtattta acagcgtttc gtaaccttca cgaccacccc acaggacata
     7921 gttttcaccg cccaatttat gggttgcttc catcgctgta acaacttgcg ttgccgccca
     7981 gctgaagact tcaggatctg ggttcgtcgc cgcacccgcg ccatagcgag ggtttgtaaa
     8041 gcagttagcg gttccccaca gcagcttcac gccgctctct tcttgcttgg ctgccaggac
     8101 atcaaccatt tgcgcaaaat tattgatgta ctcttttaac gacgcgccct caggggaaac
     8161 atccacatcg tggaagcaat aaaatggcac atgtaacttg tggaaaaact caaatgcgac
     8221 atctgcttta cgcttcgcca acgccagtgc ctcaccaggc tgctgccacg gacgattaaa
     8281 cgcccccaca ccaaacatgt ccgccccgtt ccagcagaag gtgtgccagt agcaggcggc
     8341 aaaacgcaag tgctcttcca tacgcttacc caacaccagt tcgtcgggat tgtagtgacg
     8401 gaatgctaac gggtttgagg attttgagcc ttcataacga acgcgatcga gctggtcaaa
     8461 ataggcttgc atattgaact ccataatcag gtaatgccgc gggtgatgga tgatgtcgta
     8521 atattgggca ctccctttca attgctcaat tatgttattt cacactgcta ttgagataat
     8581 tcacaagtgt gcgctcgctc gcaaaataaa atgggatgat gaaactgggt aattcctcga
     8641 agataaaaat gcaataagta caattgcgca acaaaagtaa gatctcggtc ataaatcaag
     8701 aaataaacca aaaatcgtaa tcgaaagata aaaatctgta attgttttcc cctgtttagt
     8761 tgctaaaaat tggttacgtt tatcgcggtg attgttactt attaaagctg tcctctaact
     8821 acagaaggcc ctacaccatg aaaataaaga acattctact caccctttgc acctcacttc
     8881 tgcttaccaa cgttgcggca cacgccaaag aagtcaaaat aggtatggcg attgatgatc
     8941 tccgtcttga acgctggcaa aaagatcgag atatttttgt gaaaaaggca gaatctctcg
     9001 gtgcgaaagt atttgtacag tctgcaaatg gcaatgaaga aacacaaatg tcgcagattg
     9061 aaaacatgat taaccggggt gtcgatgttc ttgtcattat tccgtataac ggtcaggtat
     9121 taagtaacgt tgtaaaagaa gccaaacaag aaggtattaa agtattagct tacgaccgta
     9181 tgattaacga tgcggatatc gatttttata tttctttcga taacgaaaaa gtcggtgaac
     9241 tgcaggcaaa agccttggtc gatattgttc cgcaaggtaa ttacttcctg atgggcggct
     9301 cgccggtaga taacaacgcc aagctgttcc gcgccggaca aatgaaagtg ttaaaacctt
     9361 acgttgattc tggaaaaatt aaagtcgttg gtgaccaatg ggttgatggc tggttaccgg
     9421 aaaacgcatt gaaaattatg gaaaacgcgc taaccgccaa taataacaaa attgatgctg
     9481 tagttgcctc aaacgatgcc accgcaggtg gggcaattca ggcattaagc gcgcaaggtt
     9541 tatcagggaa agtagcaatt tccggccagg atgcggatct cgcaggtatt aaacgtattg
     9601 ctgccggtac gcaaactatg acggtgtata aacctattac attgctggca aatactgccg
     9661 cagaaattgc cgttgaattg ggcaatggtc aggagccaaa agcagatacc tcactgaata
     9721 atggcctgaa agatgtcccc tcccgcctcc tgacaccgat cgatgtgaat aaaaacaaca
     9781 tcaaagatac ggtaattaaa gacggattcc acaaagagag agagctgtaa gcgttacgcc
     9841 ccggcgcgga gcgggggcgt gatttctctc catgccgcgt gaatgaattg gcttaggtgg
     9901 agtcgttatg ccttatctac ttgaaatgaa gaacattacc aaaaccttcg gcagtgtgaa
     9961 ggcgattgat aacgtcagct tgcggttgaa tgctggcgaa atcgtctcac tctgtgggga
    10021 aaatgggtct ggtaaatcaa cgctgatgaa agtgctgtgt ggtatttatc cccatggctc
    10081 ctacgaaggc gaaattattt ttgcgggaga agagattcag gcgagtcaca tccgcgatac
    10141 cgaacgcaaa ggtatcgcca tcattcacca ggaattggcc ctggtgaaag aattgaccgt
    10201 gctggaaaat atcttcctgg gtaacgaaat aacccacaat ggcattatgg attatgacct
    10261 gatgacgcta cgctatcaga agctgctcgc acaggtcagt ttatccattt cacctgatac
    10321 ccgcgttggc gatttagggc ttgggcaaca acaactggtt gaaattgcca aggcacttaa
    10381 taaacaggtg cgcttgttaa ttctcgatga accgacagcc tcattaactg agcaggaaac
    10441 gtcggtttta ctggatatta ttcgcgatct acaacagcac ggtatcgcct gtatttatat
    10501 ttcgcacaaa ctcaacgaag tcaaagcgat ttccgatacg atttgcgtta ttcgcgacgg
    10561 acagcacatt ggtacgcgtg atgctgccgg aatgagtgaa gacgatatta tcaccatgat
    10621 ggtcgggcga gagttaaccg cgctttaccc taatgaacca cataccaccg gagatgaaat
    10681 attacgtatt gaacatctga cggcatggca tccggtcaat cgtcatatta aacgagttaa
    10741 tgatgtctcg ttttccctga aacgtggcga aatactgggt attgccggac tcgttggtgc
    10801 cggacgtacc gagaccattc agtgcctgtt tggcgtgtgg cccggacaat gggaaggaaa
    10861 aatttatatt gatggcaaac aggtagatat tcgtaactgt cagcaagcca tcgcccaggg
    10921 gattgcgatg gtacccgaag acagaaagcg cgacggcatc gttccggtaa tggcggttgg
    10981 taaaaatatt accctcgccg cactcaataa atttaccggt ggcattagcc agcttgatga
    11041 cgcggcagag caaaaatgta ttctggaatc aattcagcaa ctcaaagtta aaacgtcgtc
    11101 ccccgacctt gctattggac gtttgagcgg cggcaatcag caaaaagcga tcctcgcccg
    11161 ctgtctgtta cttaacccgc gcatcctcat tcttgatgaa cctaccaggg gtattgatat
    11221 tggcgcgaaa tatgagatct acaaattaat taaccaactc gtccagcagg gtattgccgt
    11281 tattgtcatc tcttccgaat tacctgaagt gctcggcctt agcgatcgtg tactggtgat
    11341 gcatgaaggg aaactaaaag ccaacctgat aaatcataac ctgactcagg agcaggtgat
    11401 ggaagccgca ttgaggagcg aacatcatgt cgaaaagcaa tccgtctgaa gtgaaattgg
    11461 ccgtaccgac atccggtggc ttttctgggc tgaaatcact gaatttgcag gtcttcgtga
    11521 tgattgcagc tatcatcgca atcatgctgt tctttacctg gaccaccgat ggtgcctact
    11581 taagcgcccg taacgtctcc aacctgttac gccagaccgc gattaccggc atcctcgcgg
    11641 taggaatggt gttcgtcata atttctgctg aaatcgacct ttccgtcggc tcaatgatgg
    11701 ggctattagg tggcgtcgcg gcgatttgtg acgtctggtt aggctggcct ttgccactta
    11761 ccatcattgt gacgttggtt ctgggactgc ttctcggtgc ctggaacgga tggtgggtcg
    11821 cgtaccgcaa agtcccttca tttattgtca ccctcgcggg catgttggca tttcgcggca
    11881 tactcattgg catcaccaac ggcacgactg tatcccccac cagcgccgcg atgtcacaaa
    11941 ttgggcaaag ctatctcccc gccagtaccg gcttcatcat tggcgcgctt ggcttaatgg
    12001 cttttgttgg ttggcaatgg cgcggaagaa tgcgccgtca ggctttgggt ttacagtctc
    12061 cggcctctac cgcagtagtc ggtcgccaag ctttaaccgc tatcatcgta ttaggcgcaa
    12121 tctggctgtt gaatgattac cgtggcgttc ccactcctgt tctgctgctg acgttgctgt
    12181 tactcggcgg aatgtttatg gcaacgcgga cggcatttgg acgacgcatt tatgccatcg
    12241 gcggcaatct ggaagcagca cgtctctccg ggattaacgt tgaacgcacc aaacttgccg
    12301 tgttcgcgat taacggatta atggtagcca tcgccggatt aatccttagt tctagacttg
    12361 gcgctggttc accttctgcg ggaaatatcg ccgaactgga cgcaattgca gcatgcgtga
    12421 ttggcggcac cagcctggct ggcggtgtgg gaagcgttgc cggagcagta atgggggcat
    12481 ttatcatggc ttcactggat aacggcatga gtatgatgga tgtaccgacc ttctggcagt
    12541 atatcgttaa aggtgcgatt ctgttgctgg cagtatggat ggactccgca accaaacgcc
    12601 gttcttgatt ttgataaaaa ttttctcaaa gccggttacg tattaccggt tttgagtttt
    12661 tgcatgattc agcaggaaaa gaaccatgtt tactaaacgt caccgcatca cattactgtt
    12721 caatgccaat aaagcctatg accggcaggt agtagaaggc gtaggggaat atttacaggc
    12781 atcacaatca gaatgggata ttttcattga agaagatttc cgcgcccgca ttgataaaat
    12841 caaggactgg ttaggagatg tcgtcattgc cgacttcgac gacaaacaga tcgagcaagc
    12901 gctggctgat gtcgacgtcc ccattgttgg ggttggcggc tcgtatcacc ttgcagaaag
    12961 ttacccaccc gttcattaca ttgccaccga taactatgcg ctggttgaaa gcgcattttt
    13021 gcatttaaaa gagaaaggcg ttaaccgctt tgctttttat ggtcttccgg aatcaagcgg
    13081 caaacgttgg gccactgaac gcgaatatgc atttcgtcag cttgtcgccg aagaaaagta
    13141 tcgcggagtg gtttatcagg ggttagaaac cgcgccagag aactggcaac acgcgcaaaa
    13201 tcggctggca gactgactac aaacgctgcc accgcaaacc gggattattg ccgttactga
    13261 cgcccgagcg cggcatattc tgcaagtatg tgaacatcta catattcccg taccggaaaa
    13321 attatgcgtg attggcatcg ataacgaaga actgacccgc tatctgtcgc gtgtcgccct
    13381 ttcttcggtc gctcagggca cgcggcaaat gggctatcag gcggcaaaac tgttgcatcg
    13441 attattagat aaagaagaaa tgccgctaca gcgaattttg gtcccaccag ttcgcgtcat
    13501 tgaacggcgc tcaacagatt atcgctcgct gaccgatccc gccgttattc aggccatgca
    13561 ttacattcgt aatcacgcct gtaaagggat taaagtggat caggtactgg atgcggtcgg
    13621 gatctcgcgc tccaatcttg agaagcgttt taaagaagag gtgggtgaaa ccatccatgc
    13681 aatgattcat gccgagaagc tggagaaagc gcgcagtctg ctgatttcaa ccaccttgtc
    13741 gatcaatgag atatcgcaaa tgtgcggtta tccatcgctg caatatttct actctgtttt
    13801 taaaaaagca tatgacacaa cgccaaaaga gtatcgcgat gtaaatagcg aggtcatgtt
    13861 gtagcgatga tgagaattgt cggcgtcaca tcagggaact cagcacagcc acctgatgcg
    13921 agcaaaagcc ttatccgacc gacaaatcag ataaggctcg aggaaatgaa attacatatg
    13981 cgccgcgatt aaccgttggt tatcctggta cattgcgaac aggtagttgt tataactctt
    14041 ccctttggtc gagtagccct tcagcttgtg aatcatcgct gtggcagtca cttcctgatc
    14101 cgctttacgc agctgtgcac gcgatttacg gaacgaagag taagccgggt gcgtattcag
    14161 gttagtgaca taggcgctca ccgattcttt gacagaacta aactgtgagt accctttcac
    14221 tttacccggc gcattggtac aacgtccttt catgcatttc atgccgaaca gattgttatt
    14281 gttgcgcgcc agcttcgacg ttccccaacc gctttctgct gcagccatcg tcgccaccat
    14341 actggtaggg ataatgtcta cgcgttcaag caaggtattc cacgggattt ttcgcgtatt
    14401 accagaccac ttcaccttgt agcgtttggc gatgtctttc agacgcgcac gctcagcagg
    14461 tgaccattgg ccctgatact gttttgaaat cagccagtta cgttctgcag taatggctgc
    14521 attttggctg gtaatgtaag gcattacggt ccggagaaac gcctttttcc ttggtgttcc
    14581 ggaagggtat tttcgcaaat caggaagtga actactcttt gcactattgc gagaatactc
    14641 ttgtttactg cttacctgtt tattactagc tttagttaag tgggactttt gactcgctgt
    14701 tgttgtgtgc gtcttcgcta acacctcact cgaaaacacc agagtgagta acataagaat
    14761 catcgcccca tatcgtcgta tgggagtcaa aatcatcagg tctcctggcc ggatttaatc
    14821 attccaacac cttatatttt tcacaaattt gagagttgaa tctcaaatca tatcaaaaat
    14881 agctgtcaag agcaccccaa ggaatagtcc aaatctgaaa ctatgtcacg agttaacgat
    14941 tcagattggc gctaaatcgc agaaaatgtg ggggttatcg caaaattcag ccgttttttg
    15001 cgcgcgatcg ctcacccttg cttctcatcc tgtggactta ccgctcaggg atgagttttg
    15061 tctggcttat cgctggcaaa ctgtctgaaa tcgcagcaat aaggactcat ccaccatgaa
    15121 actcgccgcc tgttttctga cactccttcc tggcttcgcc gttgccgcca gctggacttc
    15181 tccggggttc cctgccttta gcgaacaggg aacgggaaca tttgtcagcc acgcgcagtt
    15241 gcccaaaggt acgcgtccac tcacgctaaa ttttgaccaa cagtgctggc agcctgcaga
    15301 tgcgataaaa ctcaatcaga tgctttccct gcaaccttgt agcaacacgc cgcctcaatg
    15361 gcgattgttc agggacggca aatatacgct gcaaatagac acccgctccg gtacgccaac
    15421 attgatgatt tccatccaga acgccgccga accggtagca aacctggtcc gtgaatgccc
    15481 gaaatgggat ggattaccgc tcacgctgga tgtcagcgcc actttcccgg aaggagccgc
    15541 cgtacgggat tattacagcc aacaaattgc gatagtgaag aacggtcaaa taacgttaca
    15601 acccgctgct accagcaacg gtttactcct gctggaacgg gcagaaactg acacatccgc
    15661 ccctttcgac tggcataacg ccacggttta ctttgtgctg acagatcgtt tcgaaaacgg
    15721 cgatcccagt aatgaccaga gttacggacg tcataaagac ggtatggcgg aaattggcac
    15781 ttttcacggc ggcgatttac gcggcctgac caacaaactg gattacctcc agcagttggg
    15841 cgttaatgct ttatggataa gcgccccatt tgagcaaatt cacggctggg tcggcggcgg
    15901 tacaaaaggc gatttcccgc attatgccta ccacggttat tacacacagg actggacgaa
    15961 tcttgatgcc aatatgggca acgaagccga tctacggacg ctggttgata gcgcacatca
    16021 gcgcggtatt cgtattctct ttgatgtcgt gatgaaccac accagctatg ccacgctggc
    16081 ggatatgcag gagtatcagt ttggcgcgtt atatctttct ggtgacgaag tgaaaaaaac
    16141 gctgggtgaa cgctggagcg actggaaacc tgccgccggg caaacctggc atagctttaa
    16201 cgattacatt aatttcagcg acaaaacagg ctgggataaa tggtggggaa aaaactggat
    16261 cagaacggat atcggcgatt acgacaatcc tggattcgac gatctcacta tgtcgctggc
    16321 ctttttgccg gatatcaaaa ccgaatcaac taccgcttct ggtctgccgg tgttctataa
    16381 aaacaaaacg gatactcacg ctaaagtcat cgaaggcttt acacctcgcg attacttaac
    16441 ccactggtta agtcagtggg tccgcgacta tgggattgat ggttttcggg tcgataccgc
    16501 caaacatgtt gagttgcccg cctggcagca actgaaaacc gaagccagct ccgcgcttcg
    16561 cgaatggaaa aaaactaacc ccgacaaagc attagatgac aaacctttct ggatgaccgg
    16621 tgaagcctag ggccacggcg tgatgcaaag tgactactat cgccacggct tcgatgcgat
    16681 gatcaatttc gattatcagg agcaggcggc gaaagcagtc gattgtctgg cgcagatgga
    16741 tacgacctgg cagcaaatgg cggagaaatt gcagggtttc aacgtgttga gctacctctc
    16801 gtcgcatgat acccgtctgt tccgtgaagg gggcgacaaa gcagcagagt tattactatt
    16861 agcgccaggc gcggtacaaa tcttttatgg cgatgaatcc tcgcgtccgt tcggtcctac
    16921 aggttctgat ccgctgcaag gtacacgttc ggatatgaac tggcaggatg ttagcggtaa
    16981 atctgccgcc aacgtcgcgc actggcagaa aatcagccag ttccgcgccc gccatcccgc
    17041 aattggcgcg ggcaaacaaa cgacactttc gctgaagcag ggctacggct ttgttcgtga
    17101 gcatggcgac gataaagtgc tggtcatctg ggctgggcaa cagtgacttt tccggcttcc
    17161 cgttcgtcag tacctcggga agccgccaac caggataaaa tgtcagccct aatcagcgtt
    17221 gcgggataaa gcaccgctca ctcttcaaca gaccgatttg caccccagca aatgtagcgt
    17281 tattgttacc ttccttgcta cagagttcga cagatatccc gctatgacat tctccctttt
    17341 tggtgacaaa tttacccgcc actccggcat tacgctgctg atggaagatc tgaacgacgg
    17401 tttacgcacg cctggcgcga ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat
    17461 gcaggactac ttccagacgc tactgaccga catgctggaa agtggcaaag cgactgatgc
    17521 actgtgtaac tacgacggtc cacaggggga aacggagcta ctcacactgc ttgccggaat
    17581 gctgcgcgag aagttgggtt gggatatcga accacagaat attgcactaa caaacggcag
    17641 ccagagcgcg tttttctact tatttaacct gtttgccgga cgccgtgccg atggtcgggt
    17701 caaaaaagtg ctgttcccgc tggcaccgga atacattggc tacgctgacg ccggactgga
    17761 agaagatctg tttgtctctg cgcgtccgaa tattgaactg ctgccggaag gccagtttaa
    17821 ataccacgtc gattttgagc atctgcatat tggcgaagaa accgggatga tttgcgtctc
    17881 ccggccgacg aatccaacag gcaatgtgat tactgacgaa gagttgctga agcttgacgc
    17941 gctggcgaat caacacggca ttccgctggt gattgataac gcttatggcg tcccgttccc
    18001 gggtatcatc ttcagtgaag cgcgcccgct atggaatccg aatatcgtac tgtgcatgag
    18061 tctttccaag ctggggctgc ctggctcccg ctgcggcatt atcatcgcca atgaaaaaat
    18121 catcactgcc atcaccaata tgaacggcat tatcagcctg gcacctggcg gtattggtcc
    18181 ggcgatgatg tgtgaaatga ttaagcgtaa cgatctgctg cgcctgtctg aaacagtcat
    18241 caaaccgttt tactaccagc gtgttcagga aactatcgcc atcattcgcc gctatttacc
    18301 ggaaaatcgc tgcctgattc ataaaccgga aggagccatt ttcctctggc tatggtttaa
    18361 ggatttgccc attacgaccg agcagctcta tcagcgcctg aaagcacgcg gcgtgctgat
    18421 ggtgccgggg cacaacttct tcccgggact ggataaaccg tggccgcata cgcatcaatg
    18481 tatgcgcatg aactacgtac cagagccgga gaaaattgag gcgggggtga agattctggc
    18541 ggaagagata gaaagagctt gggcagaaag tcactaaaaa ttgcctgatg cgctacgctt
    18601 atcaggccta catgatttct gcaatatatt gaatttggaa gaatttgtag gccggataag
    18661 gcgtttacgc cgcatctggc ataaacaaag cgcactattc cggcgttgag aaacgccgga
    18721 aaacgtttca aacaggctgc tgctgtagcc gatgccgctg cactttctcg acatccatgc
    18781 actgcaacgc ctttgtcggg caggcttcaa cacaggccgg ccccgtctcc cgatgccagc
    18841 acagatcgca cttaatcgcc ctcgcctttt tgcgcgaaga aacgacctcc atcgcaccaa
    18901 acgggcaagc cagcatacag cttttacagc caatgcaacg tgtttgttca acgaaaatat
    18961 gcccatgttc gcggcttatc gcgtcaacag ggcagacatt cgcgcacggt gcatcttcac
    19021 actgatgaca ggctactggc gtggtccagc tgtggtcttt aatgacacga atacgggaaa
    19081 taaactcgtc tggtgacaac gcagcgcaat cctgattctc ctgatgcgac actgcgcaag
    19141 ccacttcaca ggtacggcaa ccgatacatt tcgtcgcatc cgcaataata aaccggttca
    19201 tgatcattcc tttgcactaa aacagcagag tgccagagag cgcgtcccta ctgctttgat
    19261 ccaacgaagg aaatgttaaa gttttggcac ttgtccaaag cgttatgtga gcgcgcccag
    19321 gtcatccctg acagtaaatc ccagttcatt agaaatagcc tgcgcggttt cacgcagtgg
    19381 tttcaggaga tttttctctc ccacctgttt cagacgtgat gtcgaaagcg aaatcgacac
    19441 ggcgtacggc acccgcccat gaatatcaaa caccggaaca gcaatacagg agacgccgag
    19501 ttcgttttct tctctgtcca tcgccgctcc gctttcacga atgtgcgcca gttcgtcgaa
    19561 catcgcgggc agctcggtaa tggtattgtg ggttaacggc tggatctcat gctgatggtt
    19621 ttcccagtat gacttcacgt agtccgggtg accaaacgcc atgtagatct tgcccattgc
    19681 ggaacagtag agcggcatat gctggccaat ataggcacgg gttcgcagca tcccggttgt
    19741 gggttccagc ttataaatca aaatagcgtg atcgtcttcg cggctggaga agttaatggt
    19801 ttcaccagtg gcgatgttca gtgcctcaag atgcggagcg gcgatatgaa tgatattcag
    19861 cgaagacagc gccttctgcc cgacggcaat aaatttggta gtcaggcggt aactccctgc
    19921 ggcgggcgcg gtggtcacat agccgcagga ctgtaatccc tgcaataagc gatggacggt
    19981 actcttattt aaaccagcca gctccgaaag atgcgccaac ggacaaccgt ttggatagtt
    20041 gctcaaaatc tcaatcagca tcaacccgcg aaacagactc tggcttccgg ctggacgctc
    20101 tttttcctgc gccatctcgt tctctttttt tcccatcact tctttcccca ttttgtcgcg
    20161 tcctgatggt agcgcaaagt gtgccgtagt tcacgatctc gacagataat ttataaccac
    20221 ttgattttta tgtcttttga aattcatcaa tcagattgct ttgttaaaaa gtgatcgata
    20281 tatttgaaat ctagtttcgc atattgaaat tttaagacaa aaaagcgatc aaaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
    20761 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc atcggcacta
    20821 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga
    20881 tgttctctta cggcatgtta gaagttaacc gcctggcagg ccgtcagctc ccggtcgatg
    20941 gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc
    21001 gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg
    21061 ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat
    21121 acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc
    21181 gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa
    21241 accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta
    21301 acggcatcac tgttgatgac agcgtgtggg ccaaaatcca ggcgttatga ggagataagt
    21361 catgattttt ggacatatcg cgcagcccaa tccgtgccgt ttgcccgcag ccattgaaaa
    21421 ggcgctcgat tttctgcgcg ctaccaactt caacgtcctg gagccgggcg ttgtcgaaat
    21481 cgacggcaag aatatttatg cgcaa
//
LOCUS       DADMGC010000066        21239 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.066, whole genome shotgun sequence.
ACCESSION   DADMGC010000066 DADMGC010000000
VERSION     DADMGC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.066"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(32..1012)
                     /locus_tag="JNZ73_002382"
     CDS             complement(32..1012)
                     /locus_tag="JNZ73_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HAY8732638.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1020..1730)
                     /locus_tag="JNZ73_002383"
     CDS             complement(1020..1730)
                     /locus_tag="JNZ73_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HAY8732639.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1946..3769)
                     /gene="typA"
                     /locus_tag="JNZ73_002384"
     CDS             complement(1946..3769)
                     /gene="typA"
                     /locus_tag="JNZ73_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HAY8732640.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4142..5551
                     /gene="glnA"
                     /locus_tag="JNZ73_002385"
     CDS             4142..5551
                     /gene="glnA"
                     /locus_tag="JNZ73_002385"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HAY8732641.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5724..6773
                     /gene="glnL"
                     /locus_tag="JNZ73_002386"
     CDS             5724..6773
                     /gene="glnL"
                     /locus_tag="JNZ73_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HAY8732642.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6785..8194
                     /gene="glnG"
                     /locus_tag="JNZ73_002387"
     CDS             6785..8194
                     /gene="glnG"
                     /locus_tag="JNZ73_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HAY8732643.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8306..8416
                     /gene="yshB"
                     /locus_tag="JNZ73_002388"
     CDS             8306..8416
                     /gene="yshB"
                     /locus_tag="JNZ73_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HAY8732644.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8645..10018)
                     /gene="hemN"
                     /locus_tag="JNZ73_002389"
     CDS             complement(8645..10018)
                     /gene="hemN"
                     /locus_tag="JNZ73_002389"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAY8732645.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10207..10716)
                     /gene="yihI"
                     /locus_tag="JNZ73_002390"
     CDS             complement(10207..10716)
                     /gene="yihI"
                     /locus_tag="JNZ73_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HAY8732646.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11298..11930
                     /locus_tag="JNZ73_002391"
     CDS             11298..11930
                     /locus_tag="JNZ73_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAY8732647.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12312..15098)
                     /gene="polA"
                     /locus_tag="JNZ73_002392"
     CDS             complement(12312..15098)
                     /gene="polA"
                     /locus_tag="JNZ73_002392"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAY8732648.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYTEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15100..15342
                     /locus_tag="JNZ73_002393"
     CDS             15100..15342
                     /locus_tag="JNZ73_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732649.1"
                     /translation="MSVPVYQIMGKGCHRQDENMSFSPLFVKIFCRSAGSLANRHVDN
                     FVHKLFTSLLIYYHAFYSMMFVFIFHAVKNKSIPNN"
     gene            15462..16394
                     /locus_tag="JNZ73_002394"
     CDS             15462..16394
                     /locus_tag="JNZ73_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8732650.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLISSQRQNK"
     gene            complement(16435..17865)
                     /locus_tag="JNZ73_002395"
                     /pseudo
     CDS             complement(16435..17865)
                     /locus_tag="JNZ73_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
     gene            complement(18020..18646)
                     /gene="dsbA"
                     /locus_tag="JNZ73_002396"
     CDS             complement(18020..18646)
                     /gene="dsbA"
                     /locus_tag="JNZ73_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HAY8732651.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(18663..19649)
                     /gene="srkA"
                     /locus_tag="JNZ73_002397"
     CDS             complement(18663..19649)
                     /gene="srkA"
                     /locus_tag="JNZ73_002397"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HAY8732652.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRQTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(19726..19995)
                     /locus_tag="JNZ73_002398"
     CDS             complement(19726..19995)
                     /locus_tag="JNZ73_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HAY8732653.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20065..20649
                     /gene="mobA"
                     /locus_tag="JNZ73_002399"
     CDS             20065..20649
                     /gene="mobA"
                     /locus_tag="JNZ73_002399"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HAY8732654.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20631..21158
                     /gene="mobB"
                     /locus_tag="JNZ73_002400"
     CDS             20631..21158
                     /gene="mobB"
                     /locus_tag="JNZ73_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAY8732655.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDAAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5178 a         5346 c         5120 g         5595 t
ORIGIN      
        1 gttttattaa ctgcgtatga gttaaaactt attagtggtt cagaagatgg tttgcctctt
       61 gtttgatttg ttgtagtacg ttacgcacat gatttatctg attaattgca tcttcttttt
      121 ctttacgtaa ccgcgcatcc agtaaatgat tttctggtag gggtgtttca ctcatctggc
      181 gataagggat ccacggatta tcatcttcgt cttcaaagcg gaacgccggc gcatagtaat
      241 caacctcttc ttccaggcca tatgccgtca cctgcggctc atcatcacca aggcagataa
      301 ggtgcgttaa taacgctttg aatggcatat caccctgacc tgaaatacag gctttatgac
      361 ccaggccacc ctgttcttta acgatcaagg catctttaat atggacctgg gtaatatgcg
      421 gtgccatcgt ttttaaggcg tcgatgggat gctcatttgc attgatcatg ttagcaaaat
      481 caaacagtaa ggaaagagac tccatctcac tttctttgac cagcgacacc agctcatgac
      541 tttttaaatc ttcatgctgc tcaatcgtaa aagtcaggcc gctgtcctga tacgtttccc
      601 gtacataggc aatgtcgtta gcgataatcg ataatacgtc gcgcaaatta ccttcataac
      661 gtggataaaa acgtacggac gttgccccag ttttcaacgc gatggcgacg gcttcgtcaa
      721 tagatgcctt atctgaagcg ctggtttcaa tatgaatatc aaggttcaga cggcgggctt
      781 tatccccaaa ggcagagagt tctttatcgt ccatattacc aagagaaaaa cgctcgccat
      841 ccagaacatg gattttgacg ccacgtagat tattttcact tgcgatatca agtaaatcag
      901 caggcaacac tctttccagc cgcatattta aatgaaaggc ataagcatgt aaataaagag
      961 gaagagtgtc gacacgttgt agaatctttc ttgcgttatt tattgtcacc ataaaacacc
     1021 tacagattaa tgatgaggtc tacggcctca aatcgccaat attcaatatc tatctggaca
     1081 atattgcctt gctggtcggc gcgatgcttt tcaattaaga tcgctggcat acccgcagaa
     1141 ccgccaagat acttgctgat atcgcccggc attctgacgg gtttgaaaat caattttttt
     1201 actaccgtct ctttttggta gcacttttcc cagactgcag aaaatgagtg gttttcaagt
     1261 tgttcaataa aacccggagc aacttcagga ttaatatatg tttcgtgata gaaaacttta
     1321 tgtccttcca gcgcccccca gccggtgatg cgatagagtt cagttgatgg cgtcactgca
     1381 atcagtggcg cgagcgtctc gggaatatcg ctggtacggt ttttctcggt aaacccccaa
     1441 gaaggctctc gcccttgctc aattgcggcc cgctgaaagc tcgccgacag ctccggactg
     1501 taattaaatc gcggctgggt cacgaaccac cccttacgat ccttacggaa gattttggat
     1561 tctgcctcga ggttcaacaa cgcctggcgc agcgtcatac gtttaatgcc cagcagttct
     1621 cccagctctc tttctgaagg gagttttcca cccggcgtgg tgatgccatc tttcaaccac
     1681 cgatccagtt tctcttttgc attttctacg gtggattgat tctccgccat tgtttaatcc
     1741 ttttggttta aaccagatga agcatcttac gcttacgcca aaaaagaaag caattaacgg
     1801 tgtatgactt tgcagataat tgtgacaaag ctctctattg acgtaatcac agacttataa
     1861 gggagatact cgcattggtg tgcgctcacg acaggtaaaa aaaacctgcc atcgctggca
     1921 ggttttttat gactaaaaaa cgaaattaat cgtctttcgg tgcgcggttg gcgcggcgac
     1981 gatcgttttc cgtcaggtga cgtttacgaa tacggataga ggtcggagtc acttctacca
     2041 gttcgtcatc atcgatgaac tccagagctt gttccagagt catgcggata ggcggaacca
     2101 gaacaacagc ttcgtcagta ccggaagcac gcatgttggt cagtttctta ccggtcaggc
     2161 agtttacagt caggtcgtta gagcggctat gaataccgat aatctgacct tcgtaaactt
     2221 ctgcaccgtg accgaggaac agcttaccgc gatcctgcag accgaacagc gcgaacgcga
     2281 cagctttacc ctgaccgtta gagatcagta caccgttctg acgctgaccc acttcgcccg
     2341 gacgtacgtc gtcgtagtgg ctgaaggtgg agtacagcag accagtaccg gaagtcatgg
     2401 tcatgaactc agaacggaag ccaatcagac cacggcttgg gatcacgtag tcgagacgta
     2461 cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc tttacgttcg cccagcgcct
     2521 gcatcacaga accctgatgc tgttcttcaa cgtccagcgt cacgttttca tacggctctt
     2581 gtttacgacc gtcgatttca cggaagatta ctttcggacg ggataccgcc agttcgaaac
     2641 cttcacgacg catgttttcg ataagaacag agaggtgcag ttcaccacga ccagaaacgc
     2701 ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc aacgttgtgt accagttctt
     2761 tgttcagacg atcgaggatc tgacgagacg ttacgaactt accttcttta ccgcagaacg
     2821 gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc atcaacggag agtgccggca
     2881 gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat gttcagttcg cccaggcccg
     2941 tgatcgccac gatatcgcca gcttccgcca gatcggtttc gatacgttcc agaccgaggt
     3001 ggcccagcac tttaccgact ttcgcgttac gggttttgcc ttcgctatcg atgatagtaa
     3061 cctgctggtt cggcttcact ttaccacgct tgatgcggcc aatgccgata acgccaacat
     3121 agctgttgta atcgagctga gaaatctgca tctggaacgg accgtcaagg tcaacgtccg
     3181 gcgcaggaac gtggtcaaca atcgcctgat acagcggggt catgtcttcc gccatatctt
     3241 cgtggtccag acccgcgata ccgttcagcg cagaagcgta aacgatcggg aagtccagct
     3301 gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac ctgatccaca acccaatcag
     3361 gacgcgcgcc agggcggtca actttgttga taacaacaat cggcttcagg ccgtaagcaa
     3421 acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc gtcaaatgcg tcaaccacca
     3481 gcagcactga gtctaccatg gacattacac gttcaacttc accaccgaag tcggcgtgcc
     3541 ccggggtatc aacgatgttg atacggtaat cattccattt gatagcggtg tttttcgcga
     3601 ggatggtaat cccacgctct ttctccaaat cgttggagtc catcacgcgc tcttgggttt
     3661 cggcacgaga gtcgaacgta ccggattgtt ggagcagctt gtctaccaag gtggttttac
     3721 catggtctac gtgcgcgatg atggcgatat tacgcaattt ttcgatcaca actttgcctc
     3781 aggcattaga aatagcgcgt tattgtacac ggattaatcg cactacaaaa caggatcaca
     3841 aacatcctcc gcaaacaagt attgcagagt ccctttgtga tcgctttcac ggagcataaa
     3901 aagggttatc caaaggtcat tgcaccaata tggtgcttaa tgtttccatt gaagcactat
     3961 attggtgcaa cattcacatc atggtgcagc ccttttgcac gatgttaagc atgataacgc
     4021 cttttagggg caatttaaaa gttggcacag atttcgcttt atctttttta cggcgacacg
     4081 gccaaaataa ttgcagattt cgttaccacg acgacaatga ccaatccagg agagttaaag
     4141 tatgtccgct gaacacgtac tgacgatgct gaacgagcac gaagtgaagt ttgttgattt
     4201 gcgcttcacc gataccaaag gtaaagaaca acacgtcact atccctgctc atcaggtgaa
     4261 tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc tcgattggcg gctggaaagg
     4321 cattaacgaa tccgacatgg tgctgatgcc agacgcatcc accgcagtga ttgacccgtt
     4381 cttcgccgac tccaccctga ttatccgttg cgacatcctt gaacctggca ccctgcaagg
     4441 ctatgaccgt gacccgcgct ctattgcgaa gcgcgccgaa gattacctgc gttccactgg
     4501 cattgccgac accgtactgt tcgggccaga acctgaattc ttcctgttcg atgacatccg
     4561 tttcggatca tctatctccg gttcccacgt tgctatcgac gatatcgaag gcgcatggaa
     4621 ctcctccacc caatacgaag gtggtaacaa aggtcaccgt ccggcagtga aaggcggtta
     4681 cttcccggtt ccgccggtag actctgctca ggatattcgt tctgaaatgt gtctggtgat
     4741 ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa gtagcgactg ctggtcagaa
     4801 cgaagtggct acccgcttca ataccatgac caaaaaagct gacgaaattc agatctacaa
     4861 atatgtcgtg cataacgtag cgcaccgctt cggtaaaacc gcgaccttta tgccaaaacc
     4921 gatgttcggt gataacggct ccggtatgca ctgccacatg tctctgtcta aaaacggcgt
     4981 taacctgttc gcaggcgaca aatacgcagg tctgtctgag caggcgctgt actacattgg
     5041 cggcgtaatc aaacacgcta aagcgattaa cgccctggca aacccgacca ccaactctta
     5101 taagcgtctg gtcccgggct acgaagcacc ggtaatgctg gcttactctg cgcgtaaccg
     5161 ttctgcgtct atccgtattc cggtggtttc ttctccgaaa gcacgtcgta tcgaagtacg
     5221 tttcccggat ccggcagcta acccgtacct gtgctttgct gccctgctga tggccggtct
     5281 tgatggtatc aagaacaaga tccatccggg cgaagccatg gacaaaaacc tgtatgacct
     5341 gccgccagaa gaagcgaaag agatcccaca ggttgcaggc tctctggaag aagcactgaa
     5401 cgaactggat ctggaccgcg agttcctgaa agccggtggc gtgttcacgg atgaagcgat
     5461 cgatgcgtac atcgctctgc gtcgcgaaga agatgaccgc gtgcgtatga ctccgcatcc
     5521 ggtagagttt gagctgtact acagcgtcta atagttgaag ttgtactacc cggcgcacaa
     5581 cgccgggatt tagttgccgt ggaaactttc agcccatctc tgcatgggct tttttctccg
     5641 tcaattctct gatgcttcgc gctttttatc cgtaaaaagc tataatgcac taaaatggtg
     5701 caacctgttc aggagactgc tttatggcaa caggcacgca gcccgatgct gggcagatcc
     5761 tcaactcgct gattaacagt attttgttaa ttgatgacaa tctggcgatc cattacgcca
     5821 accccgccgc gcaacaactg ctcgcccaaa gctcccgcaa attgtttggt acgccgttac
     5881 cggaactgtt gagctacttc tcattaaata tcgagctgat gcaagaaagt ctggaggcgg
     5941 ggcaaggctt taccgataac gaagtgacgc tggtcatcga tggtcgctcg catatccttt
     6001 ctgtgacggc ccagcgtatg ccggacggca tgatcctgct ggagatggca ccgatggata
     6061 accagcgccg cttaagccag gaacaactac aacatgctca acaagtagct gcccgtgatt
     6121 tagtgcgcgg cctggcacat gagattaaaa atccgcttgg cggtttacgt ggcgcggcgc
     6181 agttactcag caaagcgtta cctgacccat cactactcga atataccaaa gtgattatcg
     6241 aacaggcgga ccggctgcga aatttggtcg accgtctgtt ggggccgcag ctgcccggta
     6301 cgcgcgttac cgaaagtatt cacaaagtgg ctgaacgcgt ggtaacgctg gtgtcgatgg
     6361 aactgccgga caacgtgcgg ttgattcgtg attacgatcc cagcctgccg gaactggcgc
     6421 acgacccgga tcaaattgaa caggtcttgc tgaatattgt gcgcaatgcg ctacaggcgc
     6481 tggggccgga aggcggtgaa atcattctgc gtacccgcac cgcgtttcaa ctgaccttac
     6541 acggcgagcg ctaccggctg gcggcgcgga ttgatgtgga agataacggg ccgggcattc
     6601 cgcctcattt gcaggatacg ctgttttacc cgatggtcag cggccgcgaa ggtggcaccg
     6661 ggcttggctt atccatcgcc cgtaatttga ttgatcagca ttcaggcaaa attgaattta
     6721 ccagttggcc aggtcatacc gagttctcgg tttacctgcc tatcaggaaa taaaggtgac
     6781 gtttatgcaa cgagggatag tctgggtagt cgatgacgat agttccatcc gttgggtgct
     6841 tgaacgtgcg ctcgctggag cgggtttaac ctgtacgaca tttgagaacg gcgcggaagt
     6901 actggaggcg ctggcgagca aaacgccgga tgtgctgctt tcagatatcc gtatgccggg
     6961 aatggacggg ctggcgctgc tcaagcagat taaacagcgc catccaatgc ttccggtcat
     7021 cattatgacc gcacattccg atctggatgc tgccgtcagc gcctatcaac aaggggcgtt
     7081 tgattatctg cccaaaccgt ttgatatcga cgaagcagtg gcgctggttg agcgcgctat
     7141 cagtcattac caggaacagc agcagccgcg taatattcag cttaacggcc caacgaccga
     7201 tatcatcggc gaagcgcaag ccatgcagga cgtgttccgt attatcggtc ggctttcgcg
     7261 ttcttctatt agcgtgctga ttaacggcga atccggcacc ggtaaagaac tggtcgctca
     7321 tgccctgcat cgccacagtc cgcgcaccaa agcgccgttt atcgcgctga atatggcagc
     7381 tatcccaaaa gatttgatcg aatcagaact gtttggccac gagaaaggcg cgtttactgg
     7441 cgcgaatacc attcgtcagg ggcgttttga acaggccgat ggcggcacgt tatttctcga
     7501 cgaaattgga gatatgccgc tggatgtgca gacgcgtttg ctgcgcgtgc tggcagacgg
     7561 tcagttttat cgcgttggcg gctatgcgcc ggtgaaagtg gatgtgcgga ttatcgctgc
     7621 cactcaccag aatcttgaac agcgggtgca ggaaggtaag ttccgtgagg atctgttcca
     7681 ccgcctgaac gttatccgcg ttcatctgcc gccgctgcgc gaacgtcggg aagatattcc
     7741 ccgtctggcg cgccattttt tacaggttgc cgcgcgcgaa ctgggcgtag aagcgaagtt
     7801 gctgcatccg gaaaccgaag ctgctctgac gcgtctggcg tggccaggca acgtgcgcca
     7861 gctggaaaac acctgccgct ggctaacggt gatggccgcc gggcaggaag tgttgattca
     7921 ggatttgccc ggcgaactgt ttgaatcaac ggttgcggag agtacttcgc aaatgcaacc
     7981 ggacagttgg gcgacacttt tagcgcagtg ggcagacaga gcgctgcgtt ccggtcatca
     8041 aaatctgctt tccgaagcgc agccagagct ggagcggacg ttactgacga ccgcgttgcg
     8101 acatacgcag gggcataaac aggaagcggc gcggctactc ggctggggcc gcaacactct
     8161 gacgcgtaag ttaaaagagc tggggatgga gtgattcacg gcttgtgtgt aaagattgat
     8221 tattgagcgc aaattgctgg tattttgcgc tttactgttc cgataagttc agtatgatct
     8281 tgcccggaaa acggggagag tcattatgct ggaatcaata attaatctgg tatcgagtgg
     8341 cgcagttgac agccacacac cgcaaactgc cgttgctgcc gtgctgtgtg ccgcgatgat
     8401 tgggctgttt agctgaagct cgttgtttat gccggatgcg gagaaagtcg taggcctgat
     8461 aagacgcgac cagcgtcgca tcaggcatca tgctcagatg cctgatgcgg cgtaaacgcc
     8521 ttatccggcc tacaaaagca ataatttcaa caggttttgt tgcccagcgc atcaggcaat
     8581 cttgttctca gcacattgat ggcgctttcg ttttctactt tgtaaacgaa gcgccattca
     8641 ctctttaaat cacccgtgag aactgctgca tccgcgcttt ctggcgcaga taggtatcaa
     8701 agcacatgca aatgttgcgg atcagcaagc gacctttcgc cgtcacctgt attcccttct
     8761 catccacatc caccagccca tcttttgcta acggggcgag cagcttgaga tcttccgcaa
     8821 agtaatcagc gaagtgcaaa tcccactgtt tctcaagagg ggcgtaatcc agacggaagt
     8881 tgcagatgag cgacttaatc acatcgcggc gaatacagtc atcacgcgtt agcgcaatac
     8941 cacgccacag cgcattgcct tgttcatcca cttgctgata gtactgcttc aactctttct
     9001 ggttctgcgc gtagcagtcg ccaatcatgc tgatggcgga aacgcccatc cccagcagat
     9061 cggtatcgcc ctgagtggtg tagccctgga agttacgatg cagcacgcct tcacgctggg
     9121 ccaccgccag ctcgtcatca ggacgggcaa agtgatccat accgataaac tgatagcccg
     9181 attgcgtcag gaaggcgatg gtttcctgca ggatatcgag tttttgctgc ggactcggca
     9241 ggtcagcatc tttgattttg cgctgagcag caaaaatggt cggcagatgc gcgtagttaa
     9301 aaacgctcag acgatcgggg ttcagctccg ccacacgttt cagggtaaag gcgaaactct
     9361 ccggcgtctg tttcggcagg ccgtaaatca ggtcgatgtt ggtggaggta aaaccaatct
     9421 cacgcgcatg gttaagcagt gcaaagatga actcttcatc ctgctcgcgg ttaaccagac
     9481 gttgcacttc tttgttgaag tcctgcacgc ccatgctcag gcgattaaag ccttcggcgc
     9541 gtaaatgatc gagtacatcc agttcgattt cccgcggatc gacttcgatc gaaatctccg
     9601 catcggtatt gaaatggaag ttttcgcgca gcagcttcat cagacggctg atttgcgctt
     9661 tattcagata cgtcggcgtt ccgccgcccc agtgcaattg gctgacgtga cgcccggcga
     9721 acagcggtgc acgatggacg atttcttgct ccagcgcgtc cagatactga tcggccttgt
     9781 gctgctggcg agtaacaatc ttattgcaac cgcagaagta acaaagctta tggcagaacg
     9841 ggatatgtac gtagagagat aatggacgct caggatagcg cgccacggct tgtaaaaacg
     9901 cctgttcgcc gaagtcttct gaaaactcca gcgcggtcgg gtacgaggtg tatcgtggcc
     9961 cggaatagtt atatttctgg atcagggcca gatcccagtc gatttgctgt acagacatgc
    10021 tcactccttc cgatggcgtc tctggcggct acggcgtacc ggcggatgcc cgtttcgggt
    10081 catcagccgg ttgcgcagcc acttttgtcg ccgcgacaac cgctgtagtt taacgaataa
    10141 ccacaccaga taacatataa ccggaagggt tatcagcaga acgggaaggc ccacggcggc
    10201 aatccgttag ttgccccgca gcagacgcat catgtcttct tgcttctcgt cttcttcctc
    10261 ttcttcgtca tcgtcataag agaggccgag tttctgcatc aactcatcaa tacgatccag
    10321 tttagcatcc acccaggatt gctcttcggc gctcagggtt tcgcctgctt ccagacgttc
    10381 cagcagcgca tccagacgct catccgtttc cagtaattcc aactccgcct gcggtgaaag
    10441 cataggtttc tcactcttcg gtttgtgctg tttggtgact ttttcagtca ctcccaatgg
    10501 aataggggtt ttactgccaa tacgtggatc ttttggtgcg ttctggcctt tgctgcctga
    10561 cgtggtgttt ccgcccgctg cgcggctgcc cggcgcatga ccacgacgtt ttttctgacg
    10621 cttacggtca cgagcttcct gatccagctc ctcgcgtgtt tttcgacgcg cttttgcatg
    10681 acctttgctg cgtgagtttg aagatgatgg tttcataatg gttcgtcttt tagccgtttt
    10741 attcagtata gatttgcggc ggaatctaac agaaagcaag caaagaaaaa aggcgacaga
    10801 ttactctgtc gccttttttc ctgactcata acccttaacg ggtcttacaa tccttgcagg
    10861 ccgccaacac accctgtttt tccattagcc ggaacaatcc gtgttgattc catttccgtt
    10921 taattacgtc tccggacgtt tgtcttcctg acaatcctct gtcttcgcct cctggcgctc
    10981 ctgaccctca tcctgagtca ttgttcctgt tagcgtcctc gctctatggg tgctacttta
    11041 cgcctttgct ttaaacaaac aatcaaccgc catcagtatt tgcacatttt cagagtttta
    11101 tataaaagca attcactgat ttatatagct tatttgacat gcatcgcaga attttctctg
    11161 ctatttcgca taaagactat cggcaatgtc tcacaaagcg tatggctttt acttacaacg
    11221 ctcacgccga aaaatccgcc ttttattatg gttcaggtat aatcgccaca aagctttgac
    11281 tgacggagac gaccgctttg actaatttga attatcaaca gacgcatttt gtgatgagtg
    11341 cgcctgatat tcgccaccta ccttccgata ccggaattga agtggctttt gcaggccgtt
    11401 ccaacgcagg taaatccagc gcgctgaaca cgctgactaa ccagaaaagc ctggctcgta
    11461 cctcaaaaac cccagggcgc acccagctta tcaacctgtt tgaagtggct gacggcaagc
    11521 gtctggttga cttgcctggg tacggttatg cggaagtccc ggaagagatg aagcgcaaat
    11581 ggcagcgtgc gctcggcgaa tacctcgaaa aacgtcagag cctgcaaggt ctggtggtgc
    11641 taatggatat tcgccatccg ctgaaagatt tggatcagca gatgattgag tgggcggtag
    11701 acagcaatat cgccgttctg gtgctgctga ccaaagcgga caaactggca agcggcgcac
    11761 gtaaagcgct attgaatatg gtgcgtgaag ctgtactggc gtttaacggt gatgtgcagg
    11821 ttgaaacgtt ttcttcgttg aagaaacaag gcgtagacaa gctgcggcag aaactggata
    11881 cctggtttag cgagatgcag cctgtagaag aaacgcagga cggcgaataa ttttcttgcc
    11941 ttaatgcttg tgccggatgt ggcgtatccg gcccgtaaat tcaatagatt acaattctac
    12001 cgttggcctg ataaacatag cgcatcaggc attacggatc ttttctttcg cccaataaaa
    12061 aacgccccag tcattactga ctggggcggc taaaatattc agccaaatcc gattacgtga
    12121 agtaaaaggt ctgaaagata gaacatctta cctctgtacc ctacgcgact aactttactc
    12181 ttttttgttc agttcagaaa gcactttttg taattttttt tcagttgttg cataggaatt
    12241 tctaatagcc atcacaaaac gtgacagctt atgttgctta cttacgaaaa aagggcatgt
    12301 tcaggcgaat cttagtgcgc ctgatcccag ttttcgccac tccccacttc caccagcaac
    12361 ggcacatcca gacgggtaca gttttccatc agttgatgaa tctgcttcgc gacggcatca
    12421 acatcatctt tatgaacttc aaataccagt tcatcgtgta cctgcatgat catacgtaca
    12481 cgcggttgct cagcctgtaa ccacgcatca acggcaatca tcgcccgttt gataatgtcg
    12541 gcggcggttc cctgcattgg cgcgttaatg gctgcacgtt cagccgctgc acgacgagca
    12601 ccattgctgg atttgatatc cggcagatac agacggcgtc cgtccagcgt ttcaacgtag
    12661 cccagctctt tcgcctgagc acgggtgcgt tccatatact ccagcacgcc agggtagcgt
    12721 tcgaagtaaa ggtccatgta cttctgcgct tctttacgtg gaatgttcaa ttgccgcgcc
    12781 agaccgaaag cactcatgcc ataaatcaga ccaaagttga tcgctttcgc gctacggcgt
    12841 tgctcgctgg tgacggtttc cagtggcaaa ccaaacactt ctgccgccgt tgcccggtgg
    12901 atatcttttc cttccgcgaa tgcggtcagc aagcctttat cacgcgagag atgcgccata
    12961 atgcgcagtt caatctgcga gtagtccgct gagacaatca cataatcctc tggcgcaata
    13021 aacgcctggc ggatacgacg accttcttcg ttacgcaccg gaatgttttg caggttagga
    13081 tcggttgacg ataaacgtcc cgttgcagtt actgcctggt gataagaggt atgcacacgc
    13141 ccggttttcg ggttgatcat cagcggcagc ttgtcggtgt aagtcgactt cagcttcgcc
    13201 agaccacgat actccagaat cacttttggc aacggatagt ccagcgccag ttcttccagt
    13261 acctcttccg acgttgacgg cgcgccaccc ggcgttttct tcagcggttt aataccctgt
    13321 ttttcaaaca gaatggtttg caactgcttg gtggaagaaa ggttaaattc ttcacctgca
    13381 atttcatgcg ctttcttttc cagctcagcc agacgcaagg tgagctcttc agaatgattg
    13441 tgcagcactt tcggatcgat cttcacaccg ttacgttcaa tgcgtgaaag caccggcacc
    13501 agcggcattt cgatattctc gaagacgttc aacggccctt tgtgtttttg cagatccggc
    13561 cacattttca gatgcaactg caaggtgaca tctgcatctt cggcggcgta acgtccggcc
    13621 tcttcgaggg caatctggtt aaaggtcagt tgatttttgc ctttacccgc aatctcttca
    13681 aaagtgatgg ttttgtgctt caaccaacgt tccgcgaggc tgtccatatc gtgacgcccg
    13741 gcaacgctat tgagaatgta ggactccagc atggtatcaa acgcaatccc acgcagttca
    13801 atgccgtaat tcgccagaat accgcgatcg tatttcaggt tttgcccgac cttcagcgcc
    13861 ttttcatctt ccagcagcgg ttttagcaac tcgagtgcac gctcgtgaga gatttgatcg
    13921 ggcgcatcaa gataatcatg agcaaccgga atatatgccg ctacgcctgg ctcgatagca
    13981 aaagaaagcc cgaccaggtt agcagagatg ttatcaaggc tgtcggtttc ggtatcaaat
    14041 gcaaataccg gcgctttttc cagcttcgca atccacgctt tcagtgtttc ttcatcaagg
    14101 atagtgacgt agttgtcata agaaatcacc gttgccgtca cttctggcgc ttcgtctgca
    14161 acactggttt cctgcggctt cgcggctggt tttgcccctt tggcctgtaa ccatttgccc
    14221 gcttcgacat cagcagtcca gcgtttgaac tcatactttt tgaacagccc caacaactct
    14281 tccgctgccg gttgctgcac ttccagttgt tcacaggtca gctccagttc aacatcggtt
    14341 ttaatcgtcg ccaactggta tgagagataa gcaacttctt tgttttgctc gagcttcgct
    14401 gccattgttt tcgcgccacg gaagctcaac ccagcaattt tttctggctc ggtatacagc
    14461 gtatccagcc cgccaagacc ttgcagcaat gcctgcgcgg ttttttcacc gacgcccggt
    14521 acgccaggaa tgttatcaga ggagtcaccc atcagcgcca ggaagtcgat gatcaattct
    14581 ggcggcacgc cgtacttatt caccacctct tccggtccga ggatggtatt cgtcatggta
    14641 ttgataagcg taatatttgg cgtcaccagc tgcgccatat ctttatcgcc agtgctaatc
    14701 agcaccggac gcccggcttt ttcggcttcg cgcgccagag taccgataac gtcgtccgct
    14761 tctacgccag aaaccgccag cagcggcagt cccatcgctt taaccatcgc gtgcagaggt
    14821 tcgatttgtg ctcgcagatc gtccggcatt ggcgggcgat gtgatttgta atgttcaaac
    14881 agttcatcac gaaaggtttt tcccttggcg tcaaagacca ccgctgcatg cgtcggttta
    14941 tattgcatga tcagactgcg cagcatgttg aggacaccat acatcgcacc ggtcggctcg
    15001 cctgcgctgt tagtcagcgg gggaaacgcg tgatatgcgc gataaagata agatgaacca
    15061 tctacaagga taagtggatt ttgggggatc tgaaccataa tgtccgtgcc tgtttatcag
    15121 attatgggta aaggatgcca cagacaggat gaaaacatga gtttttcacc gcttttcgtg
    15181 aaaatctttt gtagatctgc tggatcgctc gcaaatcgtc atgtggataa ctttgtgcat
    15241 aaattattta catcgctttt aatttattat catgcttttt acagcatgat gtttgtcttt
    15301 atttttcatg cagttaaaaa taaatccatc cctaataatt gagataatgg gattttttct
    15361 aatgtggata gatatgaatt atttttctcc ttaaggatca tccgttattt gggtcatttt
    15421 ctgctaaaat ctgcgccttg cgcttcttta gtgcactgtc tatggctaac cttttgaata
    15481 aattcattat gacgagaata ctcgctgcga taaccctttt gctgagtatc gtattgacca
    15541 ttttggtcac tattttttgt tccgtcccga tcatcattgc cgggattgta aaactattgc
    15601 tacctgtgcc agtcatttgg cgaaaggttt cgcgtttttg tgattttatg atgtattgct
    15661 ggtgtgaagg tctggcggta ttactgcatc tgaacccaca cctgcaatgg gaagttcacg
    15721 ggctggaagg gttaagtaag aagaactggt atctgcttat ttgtaaccac cgtagttggg
    15781 cagatattgt cgtactgtgc gtgctgtttc gtaagcacat tccgatgaat aaatatttcc
    15841 tcaagcaaca gctggcctgg gtgccatttc ttggcctggc gtgctgggcg ctggatatgc
    15901 cgtttatgaa gcgttattcc cgcgcttatt tattacgtca tccggagcga cgtggtcaag
    15961 atgttgaaac cactcggcgt tcttgtgaaa agtttcgcct gcatcccacc accattgtca
    16021 atttcgttga aggctcccgt ttcacgcaag aaaaacatca gcaaacccac tccacttttc
    16081 aaaacttgtt gccaccaaag gcggcaggca ttgcgatggc gctaaatgta ctgggtaaac
    16141 aattcgataa actgcttaat gttacgctgt gttatccgga caataaccgt cagccgttct
    16201 tcgatatgtt aagcggtaaa ctgacgcgga ttgtcgtaca tgtggattta cagcctattg
    16261 ccgatgagtt acatggcgac tacatcaatg acaaaagctt taagcgccat tttcagcaat
    16321 ggctgaactc attgtggcag gaaaaagacc ggcttctgac gtctctcata tcatcacagc
    16381 gtcagaataa gtaagtttta ctcgcctctg aatgagcaga ggcgagtgag tattttaatg
    16441 aacaaaacgt ccggcacgag acataaattc ttcttcgctc atctcctgtc cgttaaaaac
    16501 aactttaccc ggtgtataac gcagctgcag cgaggcggta ttgttgtcaa tcgtgattaa
    16561 ctgaaacatc tgacctaatg tcatcatccc gctgatttgt ttatcagcct gcttttgcgc
    16621 tttttccgca tccattcctt cagataaatt aagctgtttt gcggtttatg tgacgacatt
    16681 aagcggtaac ttcacatcaa aattgagcga tttgatatct ttgtttgtgg atgatgaaga
    16741 tttggctggg tcggcaatac tgatatccag attggcattt agttcgccga gtgcgttctt
    16801 ccatcttacc ggttgtttaa tggtcggctc acttttttgt aataacggta aatactcttt
    16861 gaacaatgca gcattcactt cttgcagagc aacttcatcg tttgctaact cagggtgtgc
    16921 agcaagctgc ttctgcatcg caatgttata ctggatgata aattggcgca ctgctgaggg
    16981 atcaatagat tcagcaatca atgaccactc accactaccg agttgctgat tcccttttgt
    17041 taacttatca agttcgtagg tcagattgac attgatataa tctttgtctt taacgcgatc
    17101 cagcctggtt tttgcaacga aagcatcgat ttgggcaacg tcttccccgt gattgataat
    17161 gttaatctga ttaaatttcg actcgctttc accaaccgga aatttctttt cttctaatcg
    17221 cgccagagaa tcaatggtaa aactttttgc cgttactttg ttcattggtg ataattgcag
    17281 aacaatatct tcgactttgc cttcaatatc caggttagac aatgaactac catcaccatt
    17341 aaaagtaaac tggccctcac caaaagaaaa ctttccatac tctgcagggg caacatctaa
    17401 tgtcgtagag aatgagccac caaacgcgaa tcgcaatgtc gcttccacgg gcgatttatt
    17461 tttcgcggcg ataaatagcg gttgtgtcag ttcattattc actacgttca cttttgccgc
    17521 tgccaatgct gggatgacat taccatgcat taacattgtg atgggcaacg gaccgtgctc
    17581 aacatccaca tcaaatacaa ctttctggcc tggcttgata ttgagatcgg gtgcaccgtt
    17641 atcgaatgtc atttgcattt gaatacggga attaaaaaag cctttgtcaa agtttttata
    17701 acttaacgtc atatcgtagg catgttcacc tttctttttc gcatcgttaa aatctttaat
    17761 aaacttttcg ataccaggct gaatttgcgt acctgtgtac catgtgccac caccccagat
    17821 tacggctaac gcaacaataa cacctgtagc tgacttacgt atcatataaa tattccctga
    17881 taaaacagca aaactttgaa tatccactta tgctgaaata atgtccagcg gcaggatgca
    17941 ttatcagaat gtatttatat ttattaaata ataaaaaaag cccgtgaata ttcacgggct
    18001 ttatgtaatt tacattgaat tattttttct cggacagata tttcaccgta tcagcatact
    18061 gctgaacaaa aacatccata ttgctggtat ccataccctg cggattcagc tgatatttac
    18121 cgttaacaaa catcgccgga acaccacgca attgcacatc agctgcggct ttttcctgct
    18181 gagcgaccag agatttcacc acgaagctgt tccacgccgc gtcgtactct tcacctttaa
    18241 tacctgcatt gataaataca tcgcggatat cagatgctga acgaatggtc tgggttttct
    18301 gtacgccttc aaacagcgga acagtcactt tgtcttccac gcccagcgcc atcgccacag
    18361 cccatgcctg agtcagatct ttgcccaggt caccacccat gaagttgacg tggtatttag
    18421 tcatcttcac gccttccggc agttttttct tcacattatc agaaatatgc agaacttctt
    18481 caaactgata gcagtgcggg cagaagaaag agaaaaactc cagcacttgc ggcgcgccag
    18541 ctaccggttt ttccagggta gtgtactgtt taccatcttc atactgcgcc gccgatgcgc
    18601 taaacgctaa aactaaacca gccagcgcca gccaaatctt tttcatgatc tactctctcc
    18661 gattaataca taggtgttaa ttgcaaaggg ggttcttgta gaacttttgc ctgttctata
    18721 aaagtcgccg tctgtcgtag ccagtaatct tccccggtta accacgggaa atttttcggg
    18781 aacgcgggat cagcccaatg ccgcattagc caggcaagat aataaaccaa acgcatggcg
    18841 cgtaaaggtt caatcagtcc gatttcagcg gtgtcgaact cgctaaattc ttcataagct
    18901 tcaataatag tttccagttg catccgctgc tgggctttat cgccattgag caacatccac
    18961 aaatcctgaa tagctggacc attacgtgca tcatccagat caacaaacat tggaccatcg
    19021 cgccagagaa tattcccggc gtggcagtct ccatgtagcc gcagaacggt gaaatcttcc
    19081 cgccagtgtg ctgtaacggc ggcgatcagc tcatctgtcg ctttcagaaa tgccgctttc
    19141 aacccggaag gtatcagtgt agcgtcctca aacagcttgc gaggctcaat gagatattca
    19201 ttcagaccga tggtctggcg atgaataaaa agctgtttgc gccccgtctg gtgcatacgc
    19261 cctaaataac gcccaaccgc ttccatctga tcgatattat cagcttcgaa ctggcgacca
    19321 ccgacgcttg gaaaaacagc gaaataaaat ccctgatgat tcaataaagt ctgaccgtta
    19381 aaggccacag gtgctgcgac cggaacttca tcatttacca gctgcaacgc aaattgatgt
    19441 tcttcgagaa tttgatcggc tgtccaacgt tcagggcgat aaaatttgac gacaaaacgt
    19501 cgacgctctt cgtcctgaaa ttgatagaca cggttttcat agctgttaag cggggtaaga
    19561 ccggaatcca cccggatccc ttgctcaaac agagcgtcca tgatggtatc cgggtgtagt
    19621 gtctggaaag taaaagcgct gttgttcatc ccatcatccg gaaaacatta cgaatgatga
    19681 aggatatcat ttcacgatga ttttggtggc gccgcttaca agcttttact ctttaattac
    19741 gccgcgagcg cgtaacagcg ccgttttgaa gtcttcttca taatctttct gcaaacccgg
    19801 gatcaccgca tctttcgcgg aatcacgcat tttcaggtga tagatcagaa tgtcatccgt
    19861 caaatccgcc agttcgccgt caaaacctga ctctttcgcc agtttctgca aaaattgcaa
    19921 caagttaaag tctggctctt tttgccaggc tggctggagg agttcaataa cttcattcag
    19981 acgtttacat ttcatagtaa tgctccttac tcttgagaca gacacgttag cagggtcaat
    20041 cccacaataa aagaggcgat atcggtgaat ctgatgacga cgataacagg cgttgtgctg
    20101 gcaggcggta aagccagacg aatgggcggc gtagataaag gattgctgga cttaaatggc
    20161 aaaccgctgt ggcaacatgt cgctgacgcg cttaagacgc agctctctca cgtcgtggtt
    20221 aatgctaatc gtcatcagga aatctatcag gcaggcggtc tgaaagtgat tgaagattca
    20281 ctggcggatt acccaggccc tctggcagga atgctttcag taatgcagca ggaagctggt
    20341 gagtggtttt tgttttgtcc gtgcgatacg ccttacattc cccatgattt agccgcccgg
    20401 cttaatcatc agcgcaaaga tgcgcctgtc gtgtgggtcc atgacggtga acgcgatcac
    20461 ccgactattg ctctggtaaa ccgcgctatt gagcctttat tactggaata tctgcaagca
    20521 ggagaacgcc gggtaatggc atttatgcgt ctggctggcg gtcatgcggt tgatttcagc
    20581 gatcataaag atgcatttgt taacgtgaat acgccagagg agcttgcccg atggcaggaa
    20641 aaacgatgat accgttactc gcctttgccg cgtggagtgg taccggaaaa actacgcttt
    20701 tgaaaaaact gatcccggca ttatgcgcca gagggatccg tccggggctg attaagcata
    20761 cgcaccatga tatggatgtt gataagccag gcaaagatag ctatgagctg cgcaaggctg
    20821 gcgcggcaca aaccatcgtt gccagccagc aacgatgggc cttgatgacg gaaacaccag
    20881 acgaagaaga gctggatcta cattttctcg caagtcgaat ggatacctca aagctggatt
    20941 tgattctggt cgaagggttt aagcatgaag agatcgcaaa gattgtgctg tttcgcgatg
    21001 cagccggaca tcgacctgaa gaattagtga tagacaggca tgttattgct gtagccagtg
    21061 atgtgccgct taatcttgat gtcgcattac tggatattaa tgatgttgag gggctggctg
    21121 attttgtggt ggagtggatg caaaagcaga acggataagc aagagtaggc tggatcagac
    21181 gcagccagtg tcgcatccga cctttttctg caccagcgca aaaaggcccg actgggcct
//
LOCUS       DADMGC010000067        21221 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.067, whole genome shotgun sequence.
ACCESSION   DADMGC010000067 DADMGC010000000
VERSION     DADMGC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.067"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..76
                     /locus_tag="JNZ73_002401"
     CDS             <1..76
                     /locus_tag="JNZ73_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY8732656.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            92..943
                     /locus_tag="JNZ73_002402"
                     /pseudo
     CDS             92..943
                     /locus_tag="JNZ73_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1182..1241
                     /locus_tag="JNZ73_002403"
     CDS             1182..1241
                     /locus_tag="JNZ73_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY8732657.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1558..1617
                     /gene="ibsE"
                     /locus_tag="JNZ73_002404"
     CDS             1558..1617
                     /gene="ibsE"
                     /locus_tag="JNZ73_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HAY8732658.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1737..3170)
                     /gene="hldE"
                     /locus_tag="JNZ73_002405"
     CDS             complement(1737..3170)
                     /gene="hldE"
                     /locus_tag="JNZ73_002405"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HAY8732659.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3218..6058)
                     /gene="glnE"
                     /locus_tag="JNZ73_002406"
     CDS             complement(3218..6058)
                     /gene="glnE"
                     /locus_tag="JNZ73_002406"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HAY8732660.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAAAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6081..7382)
                     /locus_tag="JNZ73_002407"
     CDS             complement(6081..7382)
                     /locus_tag="JNZ73_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HAY8732661.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIVSAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7624..8244
                     /locus_tag="JNZ73_002408"
     CDS             7624..8244
                     /locus_tag="JNZ73_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAY8732662.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8308..9546
                     /gene="cca"
                     /locus_tag="JNZ73_002409"
     CDS             8308..9546
                     /gene="cca"
                     /locus_tag="JNZ73_002409"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HAY8732663.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9709..10530)
                     /gene="bacA"
                     /locus_tag="JNZ73_002410"
     CDS             complement(9709..10530)
                     /gene="bacA"
                     /locus_tag="JNZ73_002410"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY8732664.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10621..10989)
                     /gene="folB"
                     /locus_tag="JNZ73_002411"
     CDS             complement(10621..10989)
                     /gene="folB"
                     /locus_tag="JNZ73_002411"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HAY8732665.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11094..11711
                     /gene="plsY"
                     /locus_tag="JNZ73_002412"
     CDS             11094..11711
                     /gene="plsY"
                     /locus_tag="JNZ73_002412"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAY8732666.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11724..12655)
                     /gene="ttdR"
                     /locus_tag="JNZ73_002413"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(11724..12655)
                     /gene="ttdR"
                     /locus_tag="JNZ73_002413"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            12862..13705
                     /gene="ttdA"
                     /locus_tag="JNZ73_002414"
                     /pseudo
     CDS             12862..13705
                     /gene="ttdA"
                     /locus_tag="JNZ73_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13702..14307
                     /gene="ttdB"
                     /locus_tag="JNZ73_002415"
     CDS             13702..14307
                     /gene="ttdB"
                     /locus_tag="JNZ73_002415"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HAY8732667.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14355..15752
                     /locus_tag="JNZ73_002416"
     CDS             14355..15752
                     /locus_tag="JNZ73_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HAY8732668.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPITPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLLLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALMLLLRIISWDDIVSNKAAWNVFFWLASLITLATGLNN
                     TGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRYFFASATAYTSALAPMMIAAALA
                     MPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYGSGYLPTADYWRLGAIFGLIFLV
                     LLVITGLLWMPVVLL"
     gene            complement(15795..16808)
                     /gene="tsaD"
                     /locus_tag="JNZ73_002417"
     CDS             complement(15795..16808)
                     /gene="tsaD"
                     /locus_tag="JNZ73_002417"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAY8732669.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNSTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17046..17261
                     /gene="rpsU"
                     /locus_tag="JNZ73_002418"
     CDS             17046..17261
                     /gene="rpsU"
                     /locus_tag="JNZ73_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAY8732670.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17372..19117
                     /gene="dnaG"
                     /locus_tag="JNZ73_002419"
     CDS             17372..19117
                     /gene="dnaG"
                     /locus_tag="JNZ73_002419"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY8732671.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVIFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTSGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19312..21153
                     /gene="rpoD"
                     /locus_tag="JNZ73_002420"
     CDS             19312..21153
                     /gene="rpoD"
                     /locus_tag="JNZ73_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAY8732672.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5120 a         5866 c         5442 g         4793 t
ORIGIN      
        1 actgtcgttc tcaaaatcgg tggagttgca tgacaaggtc atcgggcatt atctgaacat
       61 aaaacactat cagtaagttg gagtcattac cgaacaacaa gaagcgttta tgacgcttga
      121 gcaggagcag caggttaaaa cccgtactgc cgaacagaat gcacgtattg cggcttttga
      181 agctgaacgt cgtcgtgaag cagagcagac gcgaattctg gctgaacgac aaattcagga
      241 aacggagatc gagcgcgagc aggccgtccg ctcaagaaag gttgaggctg aacgtgaagt
      301 tcgtattaaa gagatcgaac agcagcaggt caccgaaatc gccaaccaga cgaaatcgat
      361 cgctattgcc gccaaatcgg aacaacagtc ccaggcagaa gccgatcgtg ctaaacaagt
      421 tgccctaatc gctgccgcgc aggatgcaga aaccaaagcg gttgaactga ccgtgcgggc
      481 gaaagcagaa aaagaagccg cagagatgca ggcggcggct atcgttgagt tagccgaagc
      541 tacacgtaaa aagggtctgg cggaagcaga agcacaacgt gcgctgaacg atgctatcaa
      601 cgtactttct gatgaacaaa ccagccttaa attcaaactg gccttgttgc aggcgctacc
      661 tgcggtaata gaaaaatccg ttgagccgat gaaatctatt gacggtatca agattattca
      721 ggtcgatggt ctgaatcgtg gcagcgctgc gggtgatgca aacacgggta atgtgggggg
      781 cggaaaccta gcggaacaag cattatcagc cgctctctct taccgcacac aggctccgct
      841 gattgactcc ttgctcaatg aaattggcgt ttcaggcggt tcactggcgg cattgacttc
      901 acccttaacc tcaacaactc ccgtcgccga aaacgtagaa taaattttca gctatgaacg
      961 tccctggcac aaaaagccgg ggatttttta tatctgcgtt ccgctaaaaa ctgcaaatga
     1021 tcaggtcgtt gcaacgtttt gcgtgaccgc tcggggaagg caaaactgcc tctgggaaag
     1081 cgttgcgcga ggtccggcgc tcatcaacaa tcggggagtc agcaaagggc tgaaacggga
     1141 aagcccctcc cgaggaaggg gccttacaga aggaaagggt tatgatgaag ctcgtcatca
     1201 tactgattgt gttgttactc gtaagtttcg cagcttatta acagccaatc agagggggga
     1261 gaaatcctcc ctcacccttg ttcctttact ttaggttgaa aaaaaatcag cgtcaagaag
     1321 gtttcattaa cacatatccc tggcccaaaa agccggggat tttttatatc tgcgttccgc
     1381 taaaaggtgc aaatgctcag gacgttgcag cgttttgcgt gaccgctcgg ggaaggcaaa
     1441 actgcttcag ggaaagcgtt gcgcggggtc cggcgctcat caacaatcgg ggagtcagca
     1501 aggggctgaa acgggaaagc ccctcccgag gaaggggcct tatagaagga aagggttatg
     1561 atgaagttcg tcatcatact ggttgtgttg ttactgttaa gtttcccgac ttactaacaa
     1621 ctgatcagag gggggagaaa tcctccctta cccttgttcc tttactttag gttgaaaaaa
     1681 caacagcgtc aataggcctg cctgctacga agcgagatct gtgaaccgct ttccggttag
     1741 ccttttttat cctgttggat cttcttgatg atgttagtgg tcgagcaacc gtcttcgaag
     1801 ttgagcacca gcacttcgcc gccgttggcc cagacttctt tactcccggc gatctcttct
     1861 ggtttatagt cgccgccctt caccagtaaa tctggcagga tcccggcgat caagcgctgc
     1921 ggcgtgtctt cttcaaacga caccacccag tcgaccgctt ccagtgcgcc cagcacaatc
     1981 atacgctgtt cgagcgggtt aacggggcgg gaatcccctt tcagccgttt ggtggaggca
     2041 tcgctgttca cagcgacaat caagcggtca cccagtttgc gggcatttgc cagatagaag
     2101 acgtgtccgg cgtgcaggat gtcaaagacg ccattggtca tcaccacttt ttcaccgcgt
     2161 ttacgcgctg ccgctacggc cagcttcagt tcctcttcgg tcatcacgcc aaagccggta
     2221 tctgcgcgcc cacgcactgc attttccagc tcgatcggcg aaaccgtgga ggttcccagt
     2281 ttgccgacca ccacgccagc cgccgcattg gcaaagaagc aggcttcttc cagcgaatta
     2341 cccgctgcca gcgttgccgc caggacgcca atcaccgtgt cgcccgcacc ggtaacgtca
     2401 tacacttcct gcgcctgggt tggcatatgc agcggcgctt tacccggttg cagcagcgac
     2461 ataccctgtt cggaacgggt cactaacaga gccgagagtt cgtaatcggc aatcagtttc
     2521 atgccgcgct caacaatctc ttcttcggtc ttacatttac cgacaacagc ttcaaattcc
     2581 gagagattcg gtgtcagcag cgtagcgccg cggtagcgct caaaatcggt accttttgga
     2641 tcaatcagca ccggaacgcc cgctttacgc gccagttgga tcatctgctg tacgcttgcc
     2701 agcgcacctt tggcgtagtc agaaagcacc agcgcgccaa tcgaactcag cgcctgatta
     2761 atccgctcgt gcagcggctg cggatcaaca ccttcgaaac cttcttcaaa atccagacgg
     2821 atcagctgtt ggttgcggga aagtacccgt aatttggtga tggtcggatg cgtcggtaca
     2881 gaaacgaagt cgcatttgac gttgacgtcg gccagagatt tactcagcgc gcgcgctgca
     2941 tcgtcaatgc ccgtcaaccc gaccaggcgt gcattagcac cgagagaagc gatattcatc
     3001 gccacgttag ccgcgccgcc cggacgttct tcgatggtat tcactttaac cacgggcacc
     3061 ggcgcttccg gcgagatacg actggtgggg ccgtaccagt aacgatccag catcacatca
     3121 ccaaccacca tcactcctgc acgttcaaac tctggcagcg ttactttcat tcctgtctcc
     3181 tgagagattc aaaatttgcg cgcgataata ccatacttca ttcttccacc agccacttct
     3241 gccagcttgc ccgcaccaga tcacgctctg cggtgaagca atcctccgac acatggcccg
     3301 gcaattcctg taatgccaga tgatgaagtt catcgcgcag cgtcgtgtaa gcacgggtca
     3361 gcgccatcgc ttcctgctct tccataatgt cgttttgcgc cagcagctcc agaatacgca
     3421 cgttgtctga ccagcgcgtt aacttcggtt tttcatgagc gtagcgcaac accagatatt
     3481 gggtaataaa ttcgatatcg gtaattccgc cttcatcggc tttgatatca aagcgattac
     3541 gatgtttatt gccgagatga gcgcgcattt tctcgcgcat ttcccgcact tccgtttgca
     3601 gagttttacc ttctcgcggc agtgtcataa tctcacgacg caccgcgtca aagtgcgcag
     3661 tgagctgcgg atcgccgtac actacacgcg cacgcaccag cgcctgatgt tcccacgtcc
     3721 aggcctcgtt tttctgataa tcggcaaatg ctgctgcgga tgtcaccagc attcccgccg
     3781 ccccggacgg acgcagtcga gcatccactt catacaaaat gccggaagag gtacgcgtac
     3841 tgaacagatg cataatgcgt tgcgccagac gcagataaaa ctgccgcccg tcgatttccc
     3901 gctcgccgtc agtcatcgca tccattgggc aatcatggag gaagataagg tcaagatcgg
     3961 aactgtagcc taactcccag ccgcccagct tgccgtagcc gaccaccgca aaaccgcgcc
     4021 cttcacgttc gttgaggtga tttggcttac cgtagcgggc caccatttga ccccacgcct
     4081 gctgaacgac ggcatctatc atagcttctg ccagccaggt taagtgatcg ctcactttca
     4141 ttaccggtag cgtaccggcg atatccgctg ccgcaatgcg taacagctgc gcctgtttga
     4201 actgacgcag cgcctcaagc tgttgttctt cgtcatcttc cggcacgcgc agcaaatact
     4261 ggcgcagttc atcacggtag gcatcggtcg ccgtcggctg gtaaagggta tttggatcga
     4321 gcaattcatc cagcaataat ggataacgcg ctagctggct ggcaatcatc ggcgatgcgg
     4381 cacacagaga aatcaaatgt ttaagcgccg cagggaattc actgagcaat tctaaatagg
     4441 ttgtgcgggt aacaatcccc accagcaagg cggtaatgcg cgacagcgtg acagcagcgt
     4501 cttcacgcgc acagacatca cttagcagat gtggcatcag atggtcgagc acctgacgtc
     4561 ctcgcggccc gatagtacgc ttatccagct ctttgcggaa atcagcaatc agcgttagga
     4621 cctgtttgcg atcatcctcg ctaagatgcg ccagcactgg cgtagtgtca tcttcctgca
     4681 acgcatcctg ccacagctca cgccactgtt ccgacagcga ctcttcctga gtttcacttt
     4741 cgtcgtcgcc aatcaattca ttaaacaccc ggcgcacatt ggtcatatgt gcggtcagcg
     4801 ccccggtcag ttgcggccag tcagcaaagt ccatcgccca cgccagccgc gcacggttaa
     4861 gctcatcaga aggaagcgtt tgggtttgtt cgtcgttaat gctttgcagc aggttttcca
     4921 gacgccgcag gaacagatat gccactcgca attgttcagc atcgttttca gaaagcagat
     4981 gcagcgcggc aatggcgctg agcgttggca gtaaagagcg cgactgcagc aacggttcgc
     5041 gcccaccgcg aatgagctgg aacacctgaa cgataaattc aatttcgcga atgccgcctg
     5101 cgccgagttt gatattgtcg gtcaaaccac gtcgacgcac ttcacgggca atcatccctt
     5161 tcatgttgcg cagcgactga atcacgctga aatcgatgta acgacggaaa acaaacgggc
     5221 gcagcatcgc acgcaactcg ttagcataga cgccttcgct atcgcccata atccgcgctt
     5281 tgaccatcgc gtaacgctcc cagtcgcgtc cctgctcctg gtaataatcc tccaacgcgg
     5341 caaagctcag caccagcggg ccactttcgc caaacggacg cagtcgcata tccacgcgat
     5401 agacgaagcc atcctgcgtt ggttgatcca gcactttaat cagccgctgc cccatgcggg
     5461 taaaaaactg cgcgttatcc agttcccggc gtccgccctg cgtacaacca tgttccggcc
     5521 aggcaaaaat cagatcgata tcagaggaga aattcagttc cccgccgccc agcttaccca
     5581 tgcctaagat cagcagcggt tgcgcttcgc cctgcgcatt gcacggcgtt ccccactcgc
     5641 ggcagcaggc gtcatacagc cagtcacgcg ccgcaacaat cagcgtctcc gccagataac
     5701 tgagctgctg caatatgctc tcttcagtaa ccagtgccag cgtttgcgcc catgcgatgc
     5761 gcaccataat gcgccgccgg aatagccgca gctcgcgcat taacccggct tcgtcgctca
     5821 cattactgag cgcctcctgc aaccacacgc tgtaatgttg ccattcgtca gcctgtggcg
     5881 gttggctttc cagttccgtc agccactctg gatgcgcaat cacgctgtcc tgcacaaaat
     5941 cactgaaagt aagtactgac ttcgcctgtg cactaagtga ttcctcggct aaaagctctg
     6001 gcagccgctc aacaacggtc tgccagtact gctgtaacgg tgaagagagc ggcttcataa
     6061 gcgattttat ccttgcctga ttaacgtttt ccgctgtgca accagaacgg ttcctgattg
     6121 tttgcctcat tacggaaatg ttcaatttca atgcgttgcc cggtcgcaat agcgtgacgc
     6181 agcccctgcc agttctccag ccaggcttgc gcgacgacag gatcatagta acccgccagc
     6241 aacagtattg agtcaatatc acgcatcagg cgtggcaact ggtcacggta gcgatcgcct
     6301 aacggctggc agaaaacgct tttcagttca gcggcatggc gggaaagatg gatatcggca
     6361 aagcgtttga aggagtcgct aattttgccc tgcgctttgg catctaaaaa cggctgccat
     6421 gctttgctta ccagccattc ggtcaacgcc agcttcgcca ttgccgtttc ggtagagtag
     6481 accgccgtca cggcagaaac cgcagaaacg atggtcgctt cgcattgagt cagcaaatca
     6541 cgtaagtgag tgctcgcttt acgcggcaca atgccaccga acagcatcag tgtatgacgg
     6601 actaggctaa tggctgccag cacctgctct ttcgccgcat cgttgccgcg tacccacagt
     6661 tcttcatgat actgccattg cgctaacacc agctcgagcg ccgcttccag cccctgttcc
     6721 acatcggctt ttgccgcaac atgcaaaatg gtggtcggtt tgatttcacg cgccggattg
     6781 ccctgcgcca gatgatagcc acgcgccgct ttgctcaggc tgccctggcg taatccggtt
     6841 tgcgatacca gttggttcgc cagtttcagc accgcgcgcg tgtcgccgct aagcagctcc
     6901 agttccagct cgcagatagg ctcagcaaat tcacccgctt tcacttcccc ctggtcgaga
     6961 gcgatttcaa ttcggctgcc atcgatctct actagccatt tttcgcgata aaaatcggtg
     7021 ctaaacagcg gctgcacgcg ggaagcgaga tcggcaggca attcgccgtt cggccagatt
     7081 tccgtcggta actgcgccag gtcgagcgtc ggttcgctca acgccacgtt atattccggg
     7141 cgctggtgta agccgcctgt cactcttcct gcaactttca tggtcatctc atagcgaccg
     7201 ttttcgccac gaatacgtaa gcccatatcg tgcctacgca gccagttatc cggcgtttcg
     7261 tagtaaatat tcagcaactg cacggggtca tggtgctcgc cgcccagcgt attgagatgg
     7321 tcacgcaacg cctcaacggc actgtgatta acaataaact ttaattcgat ttcctgagcc
     7381 atggccttta cttatcggtt atgtcacata agtgacgatg aacggcgaac ttaatgcgat
     7441 cttttttgtc agtagatagt attttgcgcc aaattgccat gcaacgggca atttgacggg
     7501 cgtaaaagtt tgaagcagtg gcaagacctg acacagagga ttccgattga tgtcgaatgc
     7561 tttgcgtagc gacactgatg ccactactat cgttccactt tccatgacaa taacgacagc
     7621 ctgatgccaa aattacgcct gatcggatta actttactcg cacttagcgc gactgccgtc
     7681 tcacacgctg aagaaacgcg ctatgtttct gacgaactga atacctgggt ccgtagcggt
     7741 ccgggagatc attatcgcct cgtgggcacg gttaacgccg gcgaggaagt gaccttatta
     7801 caaactgacg ccaacaccaa ttatgcccag gtgaaagaca gctctggccg taccgcctgg
     7861 atcccgttga aacaacttag cactgagcca agcctgcgct cccgtgtgcc agatctggaa
     7921 aatcaggtca aaaccctgac cgataaactc accaatatcg ataacacctg gaatcagcgc
     7981 accgcagaaa tgcagcaaaa agtggcgcag agcgacagcg tgatcaacgg gttaaaagaa
     8041 gaaaatcaga aactgaaaaa cgagctgatt gtcgcgcaga aaaaggtcga tgccgccagc
     8101 gtacagctgg atgacaaaca gcgcaccatc atcatgcaat ggtttatgta tggcggtggc
     8161 gtgctggggc ttggcttgct gctcggtctg gtactgccgc acctgatccc aagccgcaaa
     8221 cgcaaagatc gctggatgaa ctaaatcgcc ttcttcgttg cactgtctta ttattaggaa
     8281 ttgtgtattt gacgaaggga tgatggcgtg aagatttatc tggtcggtgg tgctgttcgg
     8341 gatgcattgt tagggctatc ggtcaaagac agagattggg tggtggtcgg cagtacgcca
     8401 caggagatgc tcgacgcggg ctaccagcag gtaggccgcg attttcctgt gtttctgcat
     8461 ccgcaaacgc atgaagagta tgcgctggca cgtaccgaac ggaaatccgg ttccggttac
     8521 accggtttta cttgctatgc cgcaccggat gtcacgctgg aagatgatct taagcgtcgc
     8581 gatctgacca ttaatgcgct ggcccaggac gataacggcg agattatcga cccgtacaac
     8641 ggtctgggcg atctgcaaaa tcgtctgttg cgccatgttt cccccgcttt tggcgaagat
     8701 ccgttacgcg tactgcgcgt ggcgcgtttt gctgcgcgtt atgcccacct cggttttcgt
     8761 attgccgatg aaactctgac gctgatgcgc gagatgaccc atgcgggtga actggaacac
     8821 ctgacgcctg aacgggtatg gaaagagacg gaaagcgccc tcaccacccg caatccccag
     8881 gtgttcttcc aggtactgcg cgattgcggc gcacttcgcg ttttattccc ggaaattgac
     8941 gcactgtttg gcgttccggc acctgccaag tggcatccgg aaatcgacac gggtattcat
     9001 accttaatga cgctctcaat ggcggcgatg ctgagtccgc aggttgatgt ccgtttcgca
     9061 actttatgcc acgatctcgg taaaggactg acgccgccag aactttggcc gcgtcatcat
     9121 gggcatggcc cggcgggtgt taagttagtg gaacaactat gccagcgtct gcgcgtgcca
     9181 aatgaaattc gcgatttagc cagactggtg gctgagtttc acgatctcat ccacaccttc
     9241 ccaatgctga acccaaaaac catcgtcaaa ttatttgatt ccatcgatgc ctggcgtaaa
     9301 ccgcagcgtg tcgagcaact ggcgctgacc agcgaggctg acgtgcgcgg cagaaccggt
     9361 tttgaatcag cggactaccc gcaaggccgt tggttgcgcg aaggctggga agtggcgcag
     9421 tcagtgccga caaaagcggt cgttgaagcg ggatttaaag gcgtggagat tcgcgaggag
     9481 ctgacccgac ggcggattgc ggcggtagcc agctggaagg aacaacgttg cccaaagcct
     9541 gactgagtat aaaactaaac gttagcacga accgtcccct cgccccttcg gggagagggt
     9601 tagggtgagg ggaacagacc agcaccggtg cgaacaatca cccccacccc ggccctctcc
     9661 ctacagagcg agggagaaaa cccggcctgc tcccgaacct tttctcaatc aaaagaacac
     9721 gacatacacc gcagccgcca caataaagcg ataaatggcg aacgggataa acgaaatgcg
     9781 cttaatcaat tgcaggaagg ttttaatcgc tatcagcgcc accacaaaag cggtgataaa
     9841 cccaacggca aacatcggga tatcgccggt tgtcaggaag ccccagcttt tgtagagatc
     9901 gagcgccgtt gcgcccatca tcatcggtac cgccagcagg aacgagaact cggaagcagc
     9961 gtaacggctc acgcccatca gcatcccacc tgaaatggtc gccccggaac gggagaaacc
    10021 cggccacagc gccagacact ggaaacagcc aatcataaac gcctgacgat aggtcatatc
    10081 atcaagaccc ggcgcacgcg gctctttcgg cttcaggcat tcggcggcaa tcagcaacaa
    10141 accgccaacg accagcgcat acatcacatt tatcgggtta aacaatgact taatcgtgtc
    10201 gtggaataat agccccagta ccaccgccgg aatcatcccc agcaaaatgt ggatcagcgt
    10261 taaacgacct ttgctttcac cttcgtgctg caacgggcgg ccaaagtgga tgccaatcag
    10321 gccaaacaga cgccgccaga acatcactac taccgccaga attgatccta actggatcac
    10381 aacttcaaag gttttcgccg tgtcgccctc aaaccccaac aagtgaccga caataatcat
    10441 atggcccgtg ctggataccg gcagaaattc tgtcaatcct tcgaccacac ccaatattgc
    10501 cgctatcagc agcgagtgca tatcgctcat caataaaccc ctaaattatt aaaatgtacc
    10561 gcttgtccga actactgcag tatgaccagg ttataaccgt ttggtttaac agctgtaaaa
    10621 ttaattattt tctttcagat tattgccacg ctcaatgatt acgccaacat tcgccgcccg
    10681 cgccactgcg cctggcttgc tgagtttgat acgcacccac ggcgagttga agcgtgctaa
    10741 cagcagctcc gccacctctt cagccacgcg ttccaccagc gcaaaacgcg ccccctcgac
    10801 gtggctgacc accgtttctg caatgtcagc gtaactgagg caatccgcca catcatcact
    10861 tttcgccgct ttacggttat cccatgccat ttcgatatcg aacactaact tctgttcgat
    10921 ggtctgttcc cagtcgtaaa caccaatagt ggtgattacc gaaagttgct ctataaatac
    10981 aatatccatc acgtcctgcc tgctttttgg ctaaccggat accacttccg gcgaaatgtg
    11041 cgtattatcc acagattcat cgttgaacac gaattttcaa aacggaacag cttatgagtg
    11101 caatcgcgcc tggaatgatc ctcatcgcgt acctctgcgg ctccatttcc agtgccattc
    11161 tggtttgccg cttgtgtggg ctgcccgatc cgcgaaccag cggctccggc aatccaggcg
    11221 caaccaatgt gttacgtatc ggtggcaagg gagcagccgt agcagtactg attttcgacg
    11281 ttctgaaagg aatgttgcct gtctggggcg cgtatgaatt aggtgtcagc cccttctggc
    11341 taggcttaat tgccatcgcc gcctgtcttg gacacatctg gcccgttttc ttcggtttta
    11401 aaggaggaaa aggcgttgct accgcttttg gagcaatcgc accaattggc tgggatctca
    11461 ccggagtaat ggcgggaacc tggttactga ccgtgctatt gagcggatac tcgtcgctgg
    11521 gagcgattgt cagtgcactg attgctccgt tttatgtctg gtggtttaag ccacaattca
    11581 ccttcccggt ttcgatgctc tcttgcctga tcctgctgcg tcatcatgac aacatccagc
    11641 gtctgtggcg tcgtcaggag acaaaaatct ggacgaaatt caaaagaaag cgcgaaaagg
    11701 atcccgagtg atttctggtg gatctacatg acctgatagc cttcatcggg cttgcccagc
    11761 cgttgctggc accatgccgc cagaaattcc acgcagacac gaatttcatg ctgcgataga
    11821 gcggctcccg gtaaacagcc cagatattgg cgctttgtgc atactccggc aatacttgca
    11881 ccagtttgcc actctccaga aacggcaaca catcccactc ggaacgcagc ataatccctt
    11941 tgccctccag cgcccattgc aacactatct cgccgctatt ggaggaaaga tgcccgctaa
    12001 ccttcaccga ttttttctcc tgaccgttcc ccaactccca tattccgtgg gtcatatcgc
    12061 gctctttggt caccagacag tcatgacgac ttaattcctg taatgattgt ggctgcggat
    12121 atttttgcag atactcaggt gctgcgcata atattctttt atttttcgtt aacagatgcg
    12181 caatataata atcaggaatt tcgtcattaa tacgaatatc cagatcaata ttatcctgca
    12241 ctaaatcaat ttgccgatcg aacaattcaa aatgcacctg taactcagga taattgcgca
    12301 taagttcggt aatggctggc gcaatatggc tgcgcccaaa accaaaactg caaccaatac
    12361 gaatcatccc ctccgggcgc gttttgattt gcgtgacgtc atcgaccagt cgctgatatt
    12421 gggtgaggat ttccagcgca tgttcgtaac aacgttggcc actttcagtc agcgccacgc
    12481 cgcgagcgga gcggttcagc agcgttgttg ccagcgtatt ttcaaggatt tggatccgct
    12541 tggtgacaaa tgcaggcgtt tgcccaagcg tcgccgctgc agcactaaaa ctcccgctgt
    12601 ggacgatttc taccagtacc tgcaagtctt tggctaaagg ccagctattc agcatcgtta
    12661 attatccgca gttgtgataa gcgcagtgta tttcgcaaaa cattgccatt ccctgctctg
    12721 gcgcaatatt cattcttatt ttcgcgttga ttcacgaact gttaattaca tattcacggt
    12781 ggcaaaaaat ataaaaccac atttttagtg gtagtttgtg gcggtgaatt tttccagaca
    12841 aatacaaaaa ctggagttgc catgatgagc gaaagtaata agcaacaggc agtgaataag
    12901 ttgacagaga ttgtcgctaa ctttaccgcc atgatttcta cccgaatgcc tgatgacgtg
    12961 gtggataaac taaaacagct aaaggatgcc gaaacgtcgt cgatggggaa aattatctac
    13021 catacgatgt tcgacaacat gcaaaaagcg attgacctga atcgtcctgc ctgtcaggac
    13081 accggggaga ttatgttctt cgttaaagtc ggttcccgct tcccactgct tggcgagctg
    13141 caaagcatac tcaaacaagc cgtggaagag gcaaccgtca aagcgccact acgtcacaat
    13201 gcggtagaaa tttttgacga agtaaacacc ggcaaaaata ccggtagcgg cgtaccgtgg
    13261 gtcacctggg acatcatccc cgacaatgac gatgcggaaa tcgaagttta catggcaggc
    13321 ggcggctgca cgctacctgg ccgctcgaaa gtgttaatgc cgtcagaagg ctacgaaggc
    13381 gtggtgaaat tcgtcttcga aaatatctcc accctcgccg taaacgcctg tccaccggta
    13441 ctggtgggcg tgggcatcgc cacctcggtg gaaaccgccg ccgtactctc gcgtaaagcc
    13501 attctgcgcc cgattggcag ccgccatccc aatccaaaag cggcagaact ggagctacgc
    13561 ctggaagaag gactcaaccg tctggggatt ggtccacaag ggctgaccgg caacagttca
    13621 gtgatgggcg catcgtcgcg gcacgctgct ggttcatgcc gatctcacct ttgaaaatct
    13681 gtctcacacc cggagcgcgt tatgaaaaag atcctgacaa ccccgatcaa agctgaagat
    13741 ctgcaagata ttcgcgtcgg cgatgtgatc tacctgaccg gtacgctggt gacctgccgc
    13801 gacgtttgtc accgccgttt gatcgaactg aaacgtccga tcccttacga tctcaacggc
    13861 aaagcgattt tccacgctgg ccccatcgtg cgcaaaaacg gcgacaaatg ggagatggtc
    13921 tccgtcggcc cgacaaccag tatgcgtatg gaaagttttg aacgtgaatt tattgagcag
    13981 accggcgtaa aactggtggt tggcaaaggt ggtatggggc cgctgaccga agaaggctgc
    14041 cagaaattca aggcgttaca tgtgattttc ccggcaggct gcgcggtgct ggcggcaacc
    14101 caggtagaag agattgaaga agtgcactgg acagaactcg ggatgccgga gtcactgtgg
    14161 gtctgccggg tcaaagagtt cggcccgctg attgtctcta ttgataccca cggcaacaac
    14221 ctgatagccg aaaacaaaaa gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc
    14281 tgcgagcatg tccattacat caaataaccc tcccggagag gctcacccct ctcctttttc
    14341 gcaggcataa cacgatgaaa ccttccactg aatggtggcg atatcttgca ccgctggcgg
    14401 tcatcgccat tattgctcta cttccggttc ccgcagggct ggagaaccat acctggctct
    14461 actttgccgt ttttactggc gtgatcgttg gactgatcct cgaacccgtg ccgggtgccg
    14521 tggtggcgat ggtgggtatc tccatcatcg ccatactctc tccctggctg ctgttcagcc
    14581 ccgaacagct cgcccagcca ggctttaagt tcaccgccaa atccctctcc tgggcagttt
    14641 ctggtttttc taattcggtt atctggctca ttttcgccgc ctttatgttt ggcacaggct
    14701 atgaaaaaac cgggcttgga cgccgcattg cgctgattct ggtgaaaaag atggggcatc
    14761 gcacgctgtt tctcggctat gcggtgatgt tctccgagct gatcctggca cctataacac
    14821 cgtccaactc ggcgcgtggt gcgggtatta tctatcccat catccgtaac ctgccaccgc
    14881 tctatcaatc acaaccaaac gacagcagtt cgcgcagcat tggctcgtac atcatgtgga
    14941 tggggattgt tgccgactgc gtgaccagcg ccattttcct gacggcgatg gcacctaact
    15001 tgctgttaat tggactgatg aaaagcgcat ctcacgccac gctgagttgg ggcgactggt
    15061 tcctcgggat gttgccgctc agcattttac tgcttctgct ggttccctgg ctggcttacg
    15121 tgctataccc gccggtactg aagtctggtg atcaggtgcc gcgctgggca gagacggaac
    15181 tgcaggcaat gggcccgctc tgttcgcgtg aaaaacggat gctggggctg atggtaggcg
    15241 cgctgatgct gcttctgcgc attatcagtt gggacgacat tgtcagtaat aaagcggcat
    15301 ggaacgtttt cttctggctg gcctcgctta tcaccctcgc gaccggactc aacaataccg
    15361 gttttattag ctggtttggc aaactgttag caggcagctt aagtggttat tcgccgacga
    15421 tggtgatggt gacgttgatt gtggtgtttt atctactgcg ctactttttc gccagcgcca
    15481 cggcgtatac ctccgccctc gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa
    15541 tcccgctgcc ggtgttctgc ctgatggttg gcgcggcaat tggtctgggg agcattctta
    15601 caccatacgc caccggaccc agtccgattt actacggtag tggttatctg ccaacggcgg
    15661 attactggcg actgggggcg atttttgggc tgatattcct cgtgttgctg gtgattaccg
    15721 gcttactgtg gatgcccgtg gtgttgcttt aagtgaggac ggtgccggaa attgctctcc
    15781 ggcacccaat agttttacgc agccggtaac tccgccagcg gccagcgcgg acgcacgcta
    15841 acgccgagat ccgccgtcgc gcctgcttta aaccgcacca ttccggcata ggcgatcatc
    15901 gcgccgttat cagtacaaaa ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc
    15961 atcatttcag ccagcttcgc ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc
    16021 agtcgcttaa agcccgtctg atccaacgct cgcttacact taatcatcaa cgtatcgacc
    16081 accgcatctt caaaggcgcg ggcgatatca gcacgcgtct ggtcgtcggt gctgttgtca
    16141 cgaatcgtgt tcgccgcaaa ggttttcaga ccagaaaagc tgaaatccag ccccggacgg
    16201 tcggtcatcg gacgcgggaa aacaaagcgc ccggcagtac cctgagccgc cattttcgac
    16261 agtaacggtc cgccaggata atccagcccc agcagcttcg cggttttatc aaacgcttca
    16321 ccggcggcat catcgataga ctcgccgagc agctcgtact gaccaatgcc agtcacgctg
    16381 attaactgcg tatgaccgcc ggacaccagc agcgcgacaa acggaaattc cggcgggtta
    16441 tcttccagca tcggcgctaa cagatgccct tccatatggt gtaccgggat tgccggaacg
    16501 ttccaggcaa acgccagaga gcgccccacg gttgcgccaa ccagcagtgc gccgactaat
    16561 ccagggcctg cggtataggc cacagcatca atgtcttttg ccgttaaacc agactccttt
    16621 agcgccgcct ggatcaacgg tacggtttta cgcacatgat cgcgggaggc cagttcaggc
    16681 acgacgccgc cgtagtcagc gtgcaatttc acctgactat acaattggtt ggctaacaaa
    16741 cctttttcat cgtcgtaaat ggcgatgccg gtttcatcgc aggaagtttc aatacccagt
    16801 acacgcatga cttgttttac ctcgctttat taccgcgcag tgtaggacca atgcgggttg
    16861 atgtaaaact ttgttcgccc ctggagaaag cctcgtgtat actcctcacc cttataaaag
    16921 tccctttcaa aaaaggccgc ggtgctttac aaagcagcag caattgcagt aaaattccgc
    16981 accattttga aataagctgg cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga
    17041 ggcacatgcc ggtaattaaa gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct
    17101 tcaagcgttc ctgcgaaaaa gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg
    17161 aaaaaccgac taccgaacgt aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga
    17221 aactggctcg cgaaaacgca cgccgcactc gtctgtacta attccccgag agcgttgctc
    17281 tccgatcaga ccgagttgta gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt
    17341 ttcgtttatg aattgctaaa aatcggggcc tatggctgga cgaatcccac gcgtattcat
    17401 taatgatctg ctggcacgca ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa
    17461 aaagcagggc aagaatttcc acgcgtgttg tccattccac aacgagaaaa ccccgtcatt
    17521 caccgttaac ggtgagaaac agttttacca ctgctttgga tgtggcgcgc acggcaacgc
    17581 gatcgacttc ctgatgaact acgacaagct cgagttcgtc gaaacggtcg aagagctggc
    17641 agcaatgcac aatcttgaag tgccatttga agcaggcagc ggccccagcc agatcgagcg
    17701 ccatcagagg caaacgcttt atcagttgat ggacggtctg aatacgtttt accaacaatc
    17761 tttacaacaa cctgttgcca cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca
    17821 cgaggttatc gctcgctttg cgattggttt tgcgcccccc ggctgggaca acgtcctgaa
    17881 gcggtttggc ggcaatccag aaaatcgcca gtcattgatt gatgcgggga tgttggtcac
    17941 taacgatcag ggacgcagtt acgatcgttt ccgcgaacgg gtgatattcc ccattcgcga
    18001 taaacgcggt cgggtgattg gttttggcgg gcgcgtgctg ggcaacgata cccccaaata
    18061 cctgaactcg ccggaaacag acattttcca taaaggccgc cagctttacg gtctttatga
    18121 agcgcagcag gataacgctg aacccaatcg tctgcttgtg gtcgaaggct atatggacgt
    18181 ggtggcgctg gcgcaatacg gcattaatta cgccgttgcg tcgttaggta cgtcaaccac
    18241 cgccgatcac atacaactgt tgttccgcgc gaccaacaat gtcatttgct gttatgacgg
    18301 cgaccgtgca ggccgcgatg ccgcatggcg agcgctggaa acggcgctgc cttacatgac
    18361 agacggccgt cagctacgct ttatgttttt gccggatggc gaagaccctg acacgctggt
    18421 acgaaaagaa ggtaaagaag cgtttgaagc gcggatggag caggcgatgc cactctccgc
    18481 atttctgttt aacagtctga tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg
    18541 tttgagtacg ctggcactgc cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata
    18601 tcttcgtcag gaattaggca acaaattagg catacttgat gacagccagc ttgaacgatt
    18661 aatgccaaaa gcggcagaga gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac
    18721 catgcgtata cttatagggt tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc
    18781 gcttgagaat ctggatgaaa ataagctccc tggacttggc ttattcagag aactggtcaa
    18841 cacttgtctc tcccagccag gtctgacctc cgggcaactt ttagagcact atcgtggtac
    18901 aaataatgct gccacccttg aaaaactgtc gatgtgggac gatatagcag ataagaatat
    18961 tgctgagcaa accttcaccg actcactcaa ccatatgttt gattcgctgc ttgaactgcg
    19021 ccaggaagag ttaatcgctc gtgagcgcac gcatggttta agcaacgaag aacgcctgga
    19081 gctctggaca ttaaaccagg agctggcgaa aaagtgattt aacggcttaa gtgccgaaga
    19141 gcgatcggga agcccccgac agccgcactg agaggcagcg gcaaatatat aagtacgccc
    19201 tcgtaattat cgttggcggt aaacaaccgt tggatttcag cgttaacggc tgaaggacat
    19261 cgggtcaatc gcccaacacc aacctcatga aataagtgtg gataccgtct tatggagcaa
    19321 aacccgcagt cacagctgaa acttcttgtc acccgtggta aggagcaagg ctatctgacc
    19381 tatgccgagg tcaatgacca tctgccggaa gatatcgtcg attcagatca gatcgaagac
    19441 atcatccaaa tgatcaacga catgggcatt caggtgatgg aagaagcacc ggatgccgat
    19501 gatctgatgc tggctgaaaa caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg
    19561 ctttccagcg tggaatctga aatcgggcgc acgactgacc cggtacgcat gtacatgcgt
    19621 gaaatgggca ccgttgaact gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt
    19681 gaagacggga tcaaccaagt tcaatgctcc gttgctgaat atccggaagc gatcacctat
    19741 ctgctggaac agtacgatcg tgttgaagca gaagaagcgc gtctgtccga tctgatcacc
    19801 ggctttgttg acccgaacgc agaagaagat ctggcaccta ccgccactca cgtcggttct
    19861 gagctttccc aggaagatct ggacgatgac gaagatgaag acgaagaaga tggcgatgac
    19921 gacagcgccg atgatgacaa cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa
    19981 ctacgcgctc agtacgttgt aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct
    20041 gccgctcagg aagagatcct gaaactgtct gaagtattca aacagttccg cctggtgccg
    20101 aagcagtttg actacctggt caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa
    20161 gaacgtctga tcatgaagct ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt
    20221 accctgttta ccggcaacga aaccagcgat acctggttca acgcggcaat tgcgatgaac
    20281 aagccgtggt cggaaaaact gcacgatgtc tctgaagaag tgcatcgcgc cctgcaaaaa
    20341 ctgcagcaga ttgaagaaga aaccggcctg accatcgagc aggttaaaga tatcaaccgt
    20401 cgtatgtcca tcggtgaagc gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac
    20461 ttacgtctgg ttatttctat cgccaagaaa tacaccaacc gtggcttgca gttccttgac
    20521 ctgattcagg aaggtaacat cggcctgatg aaagcggttg ataaattcga ataccgccgt
    20581 ggttacaagt tctccaccta cgcaacctgg tggatccgtc aggcgatcac ccgttctatc
    20641 gcggatcagg cgcgcaccat ccgtattccg gtgcatatga ttgagactat caacaaactc
    20701 aaccgtattt ctcgccagat gctgcaagag atgggccgtg aaccgacgcc ggaagaactg
    20761 gctgaacgta tgctgatgcc ggaagacaag atccgcaaag tgctgaagat cgccaaagag
    20821 ccaatctcca tggaaacgcc gatcggtgat gatgaagatt cgcatctggg ggatttcatc
    20881 gaggatacca ccctcgagct gccgctggat tctgcgacca ccgaaagcct gcgtgcggca
    20941 acgcacgacg tgctggctgg cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc
    21001 ggtatcgata tgaacaccga ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc
    21061 cgcgaacgta tccgtcagat cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt
    21121 tctgaagtgt tgcgtagctt cctggacgat taatcggtag gccggatcag gcgttacgcc
    21181 gcacccggca ctagtctctc tgcacaaacg ccaccttttc g
//
LOCUS       DADMGC010000068        21027 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.068, whole genome shotgun sequence.
ACCESSION   DADMGC010000068 DADMGC010000000
VERSION     DADMGC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.068"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(1..3086)
                     /locus_tag="JNZ73_002421"
                     /pseudo
     CDS             complement(1..3086)
                     /locus_tag="JNZ73_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            3384..4577
                     /locus_tag="JNZ73_002422"
     CDS             3384..4577
                     /locus_tag="JNZ73_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY8732673.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLAQAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4775..6028)
                     /locus_tag="JNZ73_002423"
     CDS             complement(4775..6028)
                     /locus_tag="JNZ73_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAY8732674.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6355..7545
                     /gene="hmpA"
                     /locus_tag="JNZ73_002424"
     CDS             6355..7545
                     /gene="hmpA"
                     /locus_tag="JNZ73_002424"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HAY8732675.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(7590..7928)
                     /gene="glnB"
                     /locus_tag="JNZ73_002425"
     CDS             complement(7590..7928)
                     /gene="glnB"
                     /locus_tag="JNZ73_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HAY8732676.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7989..9323)
                     /gene="glrR"
                     /locus_tag="JNZ73_002426"
     CDS             complement(7989..9323)
                     /gene="glrR"
                     /locus_tag="JNZ73_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HAY8732677.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9313..10026)
                     /gene="qseG"
                     /locus_tag="JNZ73_002427"
     CDS             complement(9313..10026)
                     /gene="qseG"
                     /locus_tag="JNZ73_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HAY8732678.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10191..11618)
                     /gene="qseE"
                     /locus_tag="JNZ73_002428"
     CDS             complement(10191..11618)
                     /gene="qseE"
                     /locus_tag="JNZ73_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HAY8732679.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12176..16063)
                     /gene="purL"
                     /locus_tag="JNZ73_002429"
                     /gene_synonym="purI"
     CDS             complement(12176..16063)
                     /gene="purL"
                     /locus_tag="JNZ73_002429"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAY8732680.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16321..17877
                     /gene="mltF"
                     /locus_tag="JNZ73_002430"
     CDS             16321..17877
                     /gene="mltF"
                     /locus_tag="JNZ73_002430"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HAY8732681.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17874..18410)
                     /gene="tadA"
                     /locus_tag="JNZ73_002431"
     CDS             complement(17874..18410)
                     /gene="tadA"
                     /locus_tag="JNZ73_002431"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAY8732682.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18435..19070)
                     /gene="yfhb"
                     /locus_tag="JNZ73_002432"
     CDS             complement(18435..19070)
                     /gene="yfhb"
                     /locus_tag="JNZ73_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HAY8732683.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19279..20127
                     /locus_tag="JNZ73_002433"
     CDS             19279..20127
                     /locus_tag="JNZ73_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY8732684.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(20466..21020)
                     /gene="pinE"
                     /locus_tag="JNZ73_002434"
                     /pseudo
     CDS             complement(20466..21020)
                     /gene="pinE"
                     /locus_tag="JNZ73_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
BASE COUNT         5052 a         5678 c         5353 g         4944 t
ORIGIN      
        1 gtagttaaaa ataaaaggca tcacagtggt gaataatgcc gctttcggcc aggccggatt
       61 aatcgccgtc aattcagcgc gagcagactc atattgcgca acctccccga gtcccgcgga
      121 cgccagcatc gccaccatca ggcatttttc tttatgctgc tgttgtaaat cgccatacag
      181 cgataacagg tcaggttgtg aaacagcaaa gaaatccgcc tcaatactgg ttttcgccat
      241 ctcttgcgcc cactgtttca tttcactaaa cagttgctgt gcggtttgct gttcacccag
      301 cagtcgcagc gccattcctt gccagaagag ataatcaacc ggctgatcgt tgtaataact
      361 gtggatgtta atggtgcgat cgccggtcgc cgccagacgt aaacaacgca tcgcttcagt
      421 ttcatcgccc tgcgcgttgg cgcatatcgc ctgccagaac cagatgtcgt tatcagtttg
      481 ccccggtaaa cggccttcgc ttaaattctc cggataatgc agcgcggcat gaagcagttc
      541 gctggcctgc tgcggctctc tggcatcaag atgctgccag gcgcgtaata actggttgag
      601 gataaactga ctggtgacct tcccttcccc gccttcccac gggtggaatt tacgcgtggc
      661 gagaatgtcc gccgctttgt ctgcctgacc cgtgagatgc cacaaattga gcagttctgc
      721 ggtcatgtcg tcgcgtttca gcgcaatttc cagattattt tccagacgcg ccagtcgttt
      781 ctccggtgtg gctccactta acttatccag caaatcccgt tcgaaaagca gacgtgcatc
      841 ctgcggcgca agctgataag cattatcaag ataacgcgcg gccagctcat aatcgtgttt
      901 cttattccac gcatggatcg ctaacccgcg ccagccgtcg gcaaactccg gcgacatctc
      961 tacgcaacgt tgccataagg caatggcttt gttgtagcta cgtttgttgt agtagaagca
     1021 ggccagtaaa tggcgagcaa accagcactc ttcaatactc tccagcgccg ccacttcttc
     1081 cagcgtattc gggaaacgga caaactgcgg gaagacatca atggcttttg cgaccagttc
     1141 gccacgttcg gctttcggca gcaagctggc ttgcaggtaa agcggcagcg tgcgctggca
     1201 gtccagtgcg ttcagcatct ctgccgccag agtgggcatt ccccagttaa tcagttgccc
     1261 ggcggtcatc agagcgttaa cgtcgcgtcc ctgacacaga ccgcgccact gcgcgagggc
     1321 tgattcgcta cgaccatcga accagttcag ccaccacaga gtggcgttca gcggataatc
     1381 acgcagcagt ttctcgcgct gcaaacgcgc gttgtcctga cgaccactta acaccagcag
     1441 cagattatgc aagcaaagca cttcctgatt ggttgggcag gcacgaagac tttgttggca
     1501 aaaatccaga cccgcgtcga agttatcatt acgcgccgcc agtcgtgcca gaccataata
     1561 gccgcctgct ttactattgc cgctccagac cgcacgccag aaatcctctt cggcttgttg
     1621 atattgtccc tgacgttcgt aagcactggc gcgaatcaaa cttgcttgtc cacactgcgg
     1681 gtttttgttc agcgcatgtg cgcgtcctga ctggcatacg ccaccgcttg cgggaaatct
     1741 gcgcggttat attccagcat cgccagcgcc aggttacagc gataatccag cgggtccagc
     1801 gccacgccgc gcaggtagta atcgaacggt gaacggctgg cgtgatgata ttgctccaga
     1861 tgctgaccga taaaccaggc ttcatctgta ctggtaatgt cttgtgctgc cagtggcgct
     1921 ttggcgacgt ccggcaacgg caactcctgc ggctgatgtt cctgataact cagtacaata
     1981 ttgccgtcgg catcggagag ttcaatggtc agtctgtcag gattgatacc gtgcaacacg
     2041 ccctggatgg cggtcgctgg tgtcagggcc acggcatcat cgagcaacgc gttgcatttg
     2101 ccgatttcgc ggatcgccag acggtatccg ttcaacggag agatggcata cagcccccac
     2161 tcaatccccc gctcactacg ctggagtttt atcaccgcat cgcgggaggc attttgcacc
     2221 atgcacagag aatgataagg caggaaatac tgctcgaaac gcttctcttc gtaagcatca
     2281 agccaggtaa aatcaggctg gttatcggca aaaataccgg tcatcagttc gatatacggg
     2341 ccgttattgt cggtcagact cttatcccac gcctggccaa attcactgtg tccccaactc
     2401 cactgttttt tacctggcgc aatatggtgg ttggcaacgt gcagcaaacc gccatcttca
     2461 tcgtgacacc acgcgccgac aaaatcgtac tgcgattttt cggccatata tgaggttgga
     2521 acaggcacat ttttatagcg agaaatgtcc actccggcgg agtagtccac tttgtagtaa
     2581 gtgccggtgg cgatggggaa agcggagacg gcccgtttgc cgtgatcaaa caccgcagtc
     2641 acatccggtg ggaaaacgct ctgatgccct tcaccccctt tcactgccgg gttggcccac
     2701 cacaagaaat gacgcggcgt ggcgttgccg ttatagacgc ggctggcgat ttccagtgcc
     2761 gcccggtcag ggcgcagggt gaaacctgtc atcacctgta aaccatgcat cggctccgtt
     2821 tcgccgaccc acaccgtctg tgcaccgtct tcatgggctt cgagggtgaa atcaacgggc
     2881 ataaaggtgg tcgggcgatg gtgttgcggc cagttaaact caatcccgcc ggagatccac
     2941 ggtcccagca gccccaccag cgcaggttta atgacttcat tgtgatagac aaaatcgcgc
     3001 tgtttcactt tatcccatgc gcgatgcacc cgaccgccca gttccggcag gatcatcact
     3061 ttgatgtagt cgttttccag ccacaccgcc tgctaggatt tcagggtttt ctgctcgctc
     3121 agcgtatcgg tcacgccgta gggataaacc gcgccggacg atccctgata aacgcgattt
     3181 tccaggaaca tgggatgtat atcctgcggc ccggtttcat aggtcgggat ctcaacgcgc
     3241 tcttgccaca cttttactgg agtcatggtg ccctcaatat taacaagaca tactgaatta
     3301 aaagatttgt ggcagtgtat tgaacaatct ggcaatgttt tcgcggaata atcacgcaat
     3361 taactaaaca aggtttagtg aagatgagag cctgcattaa caatcaacag attcgccacc
     3421 ataacaaatg cgtgattctg gaactgctgt accggcaaaa gcgcgccaat aaatcaacgc
     3481 tggcccggct ggcgcaaatt tcgattccgg cagtcagtaa tattttgcag gaactggaaa
     3541 gcgaaaaacg ggtggtgaat attgacgatg aaagccagac gcgcgggcat agtagcggta
     3601 catggctgat tgcgccggaa ggtgactgga cgctgtgcct gaacgtgacg cccaccagta
     3661 ttgagtgtca ggttgctaat gcttgtttaa gtccgaaagg cgaatttgag tatttacaga
     3721 ttgatgtacc gacgccgcag gcgctgctgt ccgaaatcga aaaatgctgg catcgccacc
     3781 gtaaattgtg gccggaccgc accatcaatc tggcgctggc aatccacggg caggttgatc
     3841 cggtgaccgg cgtatcgcaa accatgccgc aagcgccgtg gacaacgcca gttgaggtga
     3901 agtatctgct ggaagagaag ctgggcattc gggtgctggt cgataatgac tgcgtgatgc
     3961 tggcgcaggc ggagaaatgg caaaataatt cgcaggaacg ggatttctgc gtgatcaacg
     4021 ttgattacgg cattggctcg tcgttcgtga ttaacgagca aatttatcgc ggcagtttgt
     4081 atggtagcgg acagattggt cacaccatcg ttaatccgga tggcgtcgtc tgcgactgtg
     4141 gacgttacgg ctgcctggaa accgtcgcct cgttaagtgc attaaaaaaa caggcgcggg
     4201 tatggctaaa atcacaaccg gttaatactc agcttgatcc tgaaaaactg acaaccgcgc
     4261 agttaatcgc tgcctggcag agtggagaac cgtggatcac cagctgggtt gatcgctctg
     4321 ccaatgccat tggtttgagt ctgtataact tcctcaacat cctcaatatt aatcagattt
     4381 ggttgtacgg tcgcagttgt gcctttggtg agaactggct taatactatt attcgccaga
     4441 caggatttaa cccgttcgac cgcgacgaag gaccgagcgt gaaagcgacg caaattggct
     4501 tcgggcaatt aagccgcgca caacaggtgc tgggaattgg ctatttgtat gttgaggcgc
     4561 agttacgaca gatttgatgg cgcgataacg tagaaaggct tcccgaagga agccttgatg
     4621 atcataaacg aaaaattgcc tgatgcgcta cgcttatcag gcctacacgg agattgcaat
     4681 atattgaatt tgcaagattt tgtaggcctg agaaggtgtt catgccgcat ccaacatgaa
     4741 caacgagcac attgtcagca aatcaccgtt tcgcttatgc gtaaaccggg taacgtgcgc
     4801 agatgtcgag aactttacct ttgatgcgct cgataacggc ttcatcattg atgctgtcca
     4861 gcacgtcaca catccagcca gccagttctt tcgcttcagc ttctttaaag ccgcgacggg
     4921 taatcgccgg agtacctaca cgaataccgg aggtcacaaa cgggctcttc ggatcgttcg
     4981 gtacgctgtt tttgttgacg gtgatgttag cacggcccag agcggcgtct gcttctttac
     5041 cggtcaggtt tttatcaacc agatcaacca ggaacaggtg gttatcagtg ccgccggaaa
     5101 ccactttgta gccgcgctcg aggaacactt ctaccatcgc tttagcgttt ttcgcgacct
     5161 gctgctggta agttttgaac tcaggctcca tcgcttcttt cagagcaacc gctttaccgg
     5221 cgattacgtg catcaacgga ccgccctgac caccagggaa aacggcagag ttcagttttt
     5281 tgtacagctc ttcgctacca cctttcgcca ggatcaggcc gccgcgcgga cccgccaggg
     5341 ttttgtgagt ggtggtagta acaacgtgag catgaggaac cgggttcggg tagacgccag
     5401 cagcaaccag gcccgcaacg tgcgccatat caacgaacag ataagcaccg atgctgtcag
     5461 cgatttcacg cattttcgcc cagtcaacca cgccggaata tgcagagaaa ccaccgataa
     5521 tcattttcgg cttgtgttct ttggcttgtt tttccagatc ggcgtaatcg atatgaccgg
     5581 tagcatcgat accgtaagga acgatgttgt acagtttacc ggagaagtta accggagaac
     5641 cgtgagtcag gtgaccgcca tgcgccaggt tcatacccag aacggtatca cctggttcca
     5701 gcagcgcggt gtagaccgca aagttagcct gggagccgga gtgcggctgg acgttagcgt
     5761 agtcagcgcc gaacagttct ttcgcacgat cgatcgccag ttgttcaacg atatcaacat
     5821 actcgcaacc gccgtagtag cgtttgcccg gataaccttc agcatatttg ttggtcagct
     5881 gagaaccctg cgcctgcatt acgcgcgggc tggtgtagtt ttcggaggcg atcagttcga
     5941 tgtgctcttc ctgacgtact ttttcctgct ccatagcctg ccacagttcg gcatcataat
     6001 cggcaatgtt catttcacgc tttaacatcc gcatctcctg actcagctaa caataaaatt
     6061 tttggccttt ataggcggtc ctgttggaca acggcgaaca gtataaccga atcattgtgc
     6121 gataacaggt cttgacaaag gaatttacgc aaacgattac cttaaggcta cgcaaggctt
     6181 tggagaataa agagcttgca accggaaacg gatttctttt caggtttgtg atgcaaattt
     6241 ttcacttcat cacattcttt ctgaaaaaca ccaaagaacc atttacattg cagggctatt
     6301 ttttataaga tgcatttgag atacatcaat taagatgcaa aaaaggaaga ccatatgctt
     6361 gacgctcaaa ccatcgctac agtaaaagcc accatccctt tactggtgga aacggggcca
     6421 aagttaaccg cccatttcta cgaccgtatg tttactcata acccagaact caaagaaatt
     6481 tttaacatga gtaaccagcg taatggcgat caacgtgaag ccctgtttaa cgctattgcc
     6541 gcctacgcca gtaatattga aaacctgcct gcgctgctgc cagcggtaga aaaaatcgcg
     6601 cagaagcaca ccagcttcca gatcaaaccg gaacagtaca acatcgtcgg tgaacacctg
     6661 ttggcaacgc tggacgaaat gttcagcccg gggcaggaag tgctggacgc gtggggtaaa
     6721 gcctatggtg tactggctaa tgtatttatc aatcgcgagg cggaaatcta taacgaaaac
     6781 gccagcaaag ccggtggttg ggaaggtact cgcgatttcc gcattgtggc taaaacaccg
     6841 cgcagcgcgc ttatcaccag cttcgaactg gagccggtcg acggtggcgc agtggcagaa
     6901 taccgtccgg ggcaatatct cggcgtctgg ctgaagccgg aaggtttccc gcatcaggaa
     6961 attcgtcagt actctttgac tcgcaaaccg gatggcaaag gctatcgtat cgcggtgaaa
     7021 cgcgaagagg gtgggcaggt atccaactgg ttgcacaatc acgccaatgt tggcgatgtc
     7081 gtgaaactgg tcgctccagc aggtgatttc tttatggctg tcgcagatga cacaccagtg
     7141 acgttaatct ctaccggtgt tggtcaaacg ccaatgctgg caatgctcga cacgctggca
     7201 aaagcaggcc acacagcaca agtgaactgg ttccatgcgg cagaaaatgg cgaagttcac
     7261 gcctttgctg atgaagttaa ggaactgggg cagtcactgc cgcgctttac cgcgcacacc
     7321 tggtatcgtc agccgagcga agccgatcgc gctaaaggtc agtttgatag cgaaggtctg
     7381 atggatttga gcaaactgga aggtgcgttc agcgatccga caatgcagtt ctatctctgc
     7441 ggcccggttg gcttcatgca gtttaccgcg aaacagttag tggatctggg cgtgaagcag
     7501 gaaaacattc attacgaatg ctttggcccg cataaggtgc tgtaatttga tgttgccgga
     7561 tggaaacatc cggcaaccct tgacgcggtt taaattgccg cgtcgtcctc ttcaccggta
     7621 cggatgcgaa tgacccgagc cacgtcaaag acgaagattt taccgtcacc gattttgccg
     7681 gtttgcgccg tgcgaataat ggtatcgaca caggtatcga caatgtcgtc cggtacgaca
     7741 atctcaattt tcactttcgg cagaaaatcc accatatact ccgcgccgcg gtacagctcg
     7801 gtatggcctt tctggcgacc aaagcctttc acttcggtca ccgtcatgcc ggtaataccg
     7861 acttcggcca gtgcttcgcg gacatcgtcc agcttgaagg gttttataat cgcatcaatc
     7921 tttttcatgc tattccttga aaaggttgtc tgtcttttga tctgctaaac gtaacacata
     7981 acgccaattc attccttgaa atcgtttgca tccagctcgt gtcgggaaag cagtttataa
     8041 aattctgtcc ggttgcgccc cgccattctc gccgcgtggg tgacgttgcc tttggtgatt
     8101 tgcagcagct tacgcaaata gttgagttca aaatgattac gtgcctcaac aaaggttggc
     8161 agcgccgtat tttcaccctc cagcgcctgc tccaccagcg catcactaat caccggagat
     8221 gaggtcagcg ccacgcactg ttcaatcacg ttgaccaact ggcgcacatt acccggccag
     8281 ctcgcggtca tcaggcgttt catcgcatcg gtagagaacg cgcggacaaa cggtttatgt
     8341 cgctctgccg cctggcgcaa caggtgattt gccagtagcg gaatgtcttc tgtgcgctcc
     8401 gccagtgccg gaattttcag gctgacaacg ttgaggcggt aatagagatc ttcgcggaat
     8461 tccccgcgcg ccatcgcttt tgacagatca cggtgagtgg cagaaataat ccgcacattg
     8521 atatcaatat cgcggttact gcccagcggg cgcactttac gctcctgcaa cacgcgcagc
     8581 agtttgacct gtaacggcgc aggcatatcg ccaatttcat cgagaaatag cgttccgcct
     8641 tccgccgcct ggaataaacc ttcgcgattg ctgacagcgc cagtaaacgc gccacgcgca
     8701 tgaccaaaca gctccgactc cagcaattgc tcgggcaatg cgccacagtt aatagcaata
     8761 aatggtttgc tgttgcgcgg gctggcgttg tggatagcct gggcgaaaat ctctttcccg
     8821 gtgccgctct gaccgttaat caaaacgctg acgtctgatt gcgccaccag ccgcgcctgt
     8881 tccagcaaac gcagcatcag cgggctgcgg gtgacaattg cctcgcgcca gcgttcatcg
     8941 gtggctggcg cggattgctc cagcgcatcg tcaattgcct gatatagcgc atctttatcg
     9001 acaggcttgg tgaggaaact aaaaacgccc tgctgtgttg cagcaacggc atcggggata
     9061 gaaccatgcg cagtaagaat aattactggc attcccggct gcactttctg gatttcagca
     9121 aatagctgca taccgtccat ttcatccatc cgcaggtcgc tgataactaa atctactttt
     9181 tcgcgattca gtacccgtaa tccttcagcg ccactttccg ccgtgaccac actgtagcct
     9241 tcgctggtca ggcgcaggcc aagcagtttc agcaatcccg gatcgtcatc gaccaataat
     9301 aaatgcgcag gtttatggct catcaggagt gacctcatgg gtggatggcg cgggcttttc
     9361 actctcgtgc ggcgtatccg gcgagaaatt tccagccggt ttgcgggtcg agagctggcg
     9421 ttcaatatcg gtcaggtttt ccagcttgcg ggtggtgagt tccagttgct gctgtagaac
     9481 gtgatgttgc tggcgcaatg tatccagctc gctgtcgctg gactgctgga gtttactgta
     9541 acgttggcgc tcttccgcca gttgcagttg cagcgcctga ccgtcgcgcc agagttgata
     9601 cagtgggcga acctgtgccg ggatctcggt acttaacgct tcaatacgcg cgaccagctg
     9661 gcggcgctca tacggcgtaa ttttggcgtc ggcgagcaaa atcccttgtt taaaggtatt
     9721 ttgccagctg ccgtcgtcat attggcgagc ttgctgacgc gactgcgcag gcattaaacg
     9781 atcagcacaa tccatcgccc gcagccagta aagcggattg gtttcggttg atttaccttg
     9841 cagcgcccag atgtcgctac attcagtaga aagatagtca gccagttgat aaaccggaat
     9901 tttttcttct gctggcgtat caatggctgg cttattgtga ttctgcacgc aacccagcaa
     9961 tgccagacat ggcagccctg ccagccacag ccgtcggggc aataatcgtt gaaaaatgtg
    10021 tcgcatattc accagactta aagcctatcc cagtgggcgt aattgttgca gacagtctgg
    10081 acatggacag cgcggagaaa ccggggcgta catatcgtac gtgaagattt cgagcactac
    10141 ccggggccga actgacaaat aaaatagcct gataggatag gctgttagca ttatttcgtg
    10201 tttttcgacg acggtaattc aatgcggaaa caaacgtctt gcccgctctc gtcgaccaga
    10261 tacagttccc cttgcatacg gcgaatacaa tccctggcaa tgcttaatcc cagaccgctg
    10321 cccttcaccg ccccttttcg ctggtggctt ccctgaaaaa agggttcgaa gatcatggcg
    10381 cgttcctctt gcggaatggg cgtgcctgta ttgatgacat caatataaac ccgcgcacca
    10441 tgtaaactgc tgcgaaggca aatgttaccg gattcagccc cgtagtgcac cgcattggag
    10501 taaagattat ccagtacgct catcagcagc attggctccg ccaagcaagc tgttgctttg
    10561 agatcgacgt cggtatgcat cattttagcc cgtgcgggca ggctatgagc agaaaccact
    10621 gtctccacca gcggtgctaa ttcaacattc tccagttcca ccgcactgtc cgcctgttta
    10681 cggttgtaat caagcagttg ttcgatcagt ttttgcaaat tgcagctgct gctatcaaga
    10741 atgctcacca cctctttttg ctctggcgta agcggcccga caacctgatc agccagtaat
    10801 tcagtgccct cgcgcatact cgccagtggc gtttttaatt catgagataa atgtcttaaa
    10861 aattgatggc gttgggattc cagccatgac aggcgctcac ttaaccaaag aatacgttgc
    10921 ccaaccgagc gtaactcgct cggtccacag aacgaaacgc tattgcccag agaacgccct
    10981 tcccccagac ggttgatcat gcgctcgata tttttcaccg gcccgataat catccgcgtg
    11041 aaaagtagca ccatcaccag actcaccaga aatagcacca gcgattgcca accaaaatat
    11101 tgcccacgtt cggcgatttc acgctgaagt tgctgcccac gagagaacac cactgtgcgc
    11161 gtggcctgca ccatttcggt attggcactg gcaaaggctt caagacgcgc ggcggcggca
    11221 gcatcgggac cactgttgtt acactgaagt tgggccagat tgttcaaatc ctgacgcaat
    11281 gcctggtaaa gtttatcgtc tggcagtacg cctgcgtggg catcgagcat ttcgctgtaa
    11341 cgcttgcgct ggctttgata aaccttcgca agcgtagggt cgtccagcac gcaatactga
    11401 cggtagctac gctccatctc cagcgccgcg ttggtcatcg cttcactacg ccgggcatca
    11461 ataagcgtag tgcggttaac cagcgccgcc tgatcgctaa gcgcattcag gctttgccag
    11521 gcttgccatg ccagcaccaa caggggtagt agaattagca aaaatgccag cattaccagt
    11581 tgtcgtaatg agcggggaaa aacgggccag cgtttcaagg tgttactctc gtcagacgcg
    11641 aatagcctga tgctaaccga ggggaagttc agatacaaca aagccgggaa ttacccggct
    11701 ttgttatgga ataaggcggt gcctaactcg acgtttcgcc cgatggttga tatagctacg
    11761 ctgatatcag aagttggacg gcaggcacct tgttgtgcgt cattcgaatt ttatgtagca
    11821 cgtcccgaag gggctgacat aagtcggtga atgagccact gcttactatt atgcagtaac
    11881 tgtgccaata aagaaaatag tttggtaacg tactgattat acgttgcttt gagagattta
    11941 tggcttcttc gctatttgaa tgtaaatcag ccactgtgtc gctaaaaaga gacaacttaa
    12001 gataaactta ttagattaca ataaaatcaa tcatttaagt gtcgccagaa agcgacacgg
    12061 taaaccaccc attgtcgtga tttacagaca caaaaaagct cccagagttg ggagcttatg
    12121 atagtggttg gtgcttaatg ccgcatccgg gctgcaaaac caatgaactg atgacttacc
    12181 ccaactgctt acgcgcattg cggaaaatgc gcatccatgg gccatcctcg ccccagtttt
    12241 ccggatgcca ggagttgctg acagtacgga aaacacgttc cgggtgcggc atcataatgg
    12301 tgactcgacc actttcagtc gtaaccgccg taataccgtt cggtgaaccg ttcgggttag
    12361 ccgggtaggt ttcagtgact ttgccgaagt tatcgacata gcgcagtgcc accagccctt
    12421 tgctttccag tgccgccaga tgcgccgcat cacgcacttc cacgcgccct tcaccatgag
    12481 agacagcaat cggcatttgc gagcccacca tcccctgcaa cagcagagac gggctttggg
    12541 ttacttcaac caggctgaaa cgcgcttcaa agcgatcgga ggtgttacgc acaaaacgtg
    12601 gccacaactc gctccccggg atcagttcac gcaggttaga catcatctgg caaccgttac
    12661 atacccccag ggccagcgtt tgcggacgat ggaagaaggt tgcaaactca tcgcgtacac
    12721 ggtcattgaa caggatagac ttcgcccaac cttcaccggc acccagcaca tcaccgtagg
    12781 agaaacctcc gcacgcgacc agggcgtgaa aatcctccag tcccgtacgt ccagtcagca
    12841 ggtcgctcat atgcacgtcg atagcatcaa agcccccacg ttggaaagct gccgccattt
    12901 caacatgcga gttcacgccc tgctcacgca gtacggcaac tttcggacgt gcgccagtgg
    12961 caatatacgg tgctgccaca tcttcgttga tatcgaacga cagttttaca ttcaggcccg
    13021 gatcggcgtc gttagatttc gcctgatgct cctgatcggc acactccggg ttgtcacgca
    13081 ggcgctgcat ctgccaggta gtttctgccc accagacacg caacgtagtg cggctttcgc
    13141 tgaatacagt ctgcccgttg gcggtaatca caaaacggtc accggaaacc gcctgcccaa
    13201 cataatggac gcaatccgca agcccatgct gtgccagtac ggactcgacc gcttcacggt
    13261 cagcggcacg aacctgaatc accgcaccca gttcttcgtt aaacaacgcc gccaggcgat
    13321 cgtcacccag agaggcgata tccgcgtcaa tgccacaatg acctgcaaag gccatttccg
    13381 ccagcgttac cagcaggccg ccatcagagc ggtcgtgata cgccagcagc ttacgctgtg
    13441 caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg gacaacatcg cgtacatctg
    13501 ccggtttgtc gccaagctga cgataaacct gcgccagcgc cgttgcgccc agcgcgttat
    13561 tgcctttgcc caaatcaatc agcagcagtg cgttatcttc ggtacaaagc tgcggcgtga
    13621 tggtgtgacg tacatcttcc acgcgggcaa atgcagaaat caccagcgac agcggcgacg
    13681 tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt catcgacata gagtctttac
    13741 ccaccgggat cgtcaggccc agcgccggac aaagttcttc acccacggct ttaacggctt
    13801 catacaggcc cgcatcttca ccagggtggc ctgccgccgc catccagttg gcggaaagtt
    13861 taatgcgttt gatatcgcca atctgtgttg cggcaatatt ggttaacgct tcaccgaccg
    13921 ccagacgggc agaggcggcg aaatccagca gcgcaaccgg cgcacgctcg ccaatcgcca
    13981 tcgcttcacc gtagtagctg tcgaggctgg cggtagtgac cgcgcagtta gcgaccggca
    14041 cctgccacgg ccccaccatc tgatcgcgcg ccaccatgcc ggttacgctg cggtcaccga
    14101 tggtcaccag gaaagttttc tccgccacgg tcggcagatg cagtacgcgt ttcaccgcgt
    14161 ctgcaatggt gatcccttca cggaccagcg catcgccctt cgctttcagc gtttgtacat
    14221 cgcgggtcat cttcggcgtt ttaccaagca ggacgtccag cggcagatcg atcggctgat
    14281 tatcaaaatg acgatcgtgc agagaaagat gcagttcttc ggtcgcttca ccaatcaccg
    14341 cgtagggtgc gcgctcacgc ttacacagtt cgtcaaacag cggtaattga tcggcagcaa
    14401 ccgccagcac gtagcgttcc tgggattcgt tacaccagat ttccagcggg ctcatgcccg
    14461 gttcgtcgct tagaatatcg cgcagttcaa atttaccgcc gcgtccgccg tcgctcacca
    14521 gctccggcat cgcgttagaa agaccgcccg caccgacgtc gtggataaac agaattgggt
    14581 tagcatcacc aagctgccag caacggtcga tcacttcctg gcaacgacgc tccatctccg
    14641 ggttgtcgcg ctgtacggaa gcaaagtcga ggtcggcatc agactgacca gacgccatgg
    14701 aagacgccgc gccgccgcca agaccgatgt tcattgccgg gccgccgaga acgaccagct
    14761 tcgcaccgac gttgatctcg cctttttgta cgtgatcggc gcgaatgttg ccgatcccgc
    14821 ccgccagcat gatcggtttg tgataaccgc gcagctcttc gccattgtgg ctgttcactt
    14881 tttcttcata agtacggaag tagccgttca gtgccggacg accaaattcg ttgttaaacg
    14941 ccgcaccgcc cagcgggcct tcggtcatga tgtccagcgc ggtaacaatg cgctcaggct
    15001 taccgaaatc ttcttcccac ggctgttcga agccaggaat tcgcaggttg gatacggaga
    15061 aaccaaccaa accagctttc ggcttcgcgc cgcgcccggt ggcaccttca tcgcggattt
    15121 caccgccgga accggtcgcc gcgcccggcc acggagaaat cgccgtcggg tggttgtgag
    15181 tttcgacttt catcaggatg tgcgccggtt cctgatggaa attgtagcgg cccgtttcgt
    15241 ggtcagcaaa gtagcggccc acttcagaac cttccattac ggcggcgtta tctttataag
    15301 cagagagaac gtgatctggc gtggtttcga aagtattttt gatcatcctg aacagcgatt
    15361 tcggctgttg ttcaccgtcg ataatccagt cggcgttaaa aattttgtgg cggcagtgct
    15421 cagagttcgc ctgagcgaac atatacagct cgatgtcgtt cgggttacga ccaagctttg
    15481 tgaaagcatc ctgcagatag tcaatttcat cttccgccag agccaggcca agacgcaggt
    15541 tagcgtcgat cagcgcctga cggccctgcc ccagcaaatc aacgcttgtg accggagtcg
    15601 gttgatggtg ggcaaacaac tgctctgcat catctaaatc aaaaaagacc gtttccatca
    15661 tgcggtcgtg cagttcagcg gtaacctgct gccattgttc attggtcagc gtaccggctt
    15721 ctatatagta agcaacgccg cgctcaaggc ggtttacctg ttgtagcccg cagttatggg
    15781 caatatcggt cgctttcgaa gaccagggag agatggtgcc aggacgcggg gtcaccagca
    15841 ggagtttgcc ttgcggggcg tggctggcga gtgccggacc atatttcagc aggcgttcaa
    15901 gttgtgcgtg ctcatcatcg tttaacggcg cattgaggtc agcaaaatgg acatactcgg
    15961 cgtaaatatt gtgaaccggg agcctggcag cctgaaaacg tgccagcagt ttgttgattc
    16021 ggaatgccga cagtgcaggc gaaccacgca gaatttccat cataagtctc tcgtcttcta
    16081 agctttcggt gtacccaagg gggggaaacg ggcgtcatta taaagaatct gatgcgctga
    16141 cgaaaccgtt tgcgtggaaa taaaatcacc atcgtgaatt agcaacgcgt gccgccaatg
    16201 gctgtaataa gttgccatct ggcgcaggtt tacgcaaaat gccgctcatt tatgagtaaa
    16261 cctttcacta ttattacgtt ttttcaagct gggacgcgca cgacacagag aattaactaa
    16321 ttgaaaaaat taaagattaa ttatctgttc atcggcattc tggcactgct gctcgcggtc
    16381 gctctctggc catccattcc ctggtttggt aaagccgaca accgtatcgc cgccattcaa
    16441 gcgcggggag agttgcgtgt gagcaccatt catactcccc tgacttataa cgaaatcaac
    16501 gggaaacctt ttggcctgga ttacgaactg gcgaaacagt ttgccgatta cctcggcgta
    16561 aaactgaaag tgaccgtgcg gcagaatatc agccagctgt ttgacgacct tgataatggt
    16621 aacgccgacc tgctggcggc aggactggtc tataacagtg agcgggtaaa aaattatcag
    16681 cctggcccta cctattattc cgtgtcacaa caactggttt ataaagtggg tcagtatcgc
    16741 ccacgtacgc tgggcaacct gacggcggag caactcaccg ttgcaccggg tcatgtggtg
    16801 gttaacgatc tccagaccct gaaagaaaca aaattcccgg aattaagctg gaaggtagac
    16861 gacaaaaaag gctctgcgga attaatggaa gatgtcatcg aaggaaaact cgattacacc
    16921 attgctgatt ctgtcgccat cagcctgttt cagcgcgttc acccggagct cgccgtagcg
    16981 ctcgatatca ccgatgaaca accggtgact tggtttagcc cgttagatgg cgataatacc
    17041 ctttccgccg ccctgctcga cttcttcaac gaaatgaatg aagacggtac gctggcacgc
    17101 attgaagaga aatacctggg gcatggcgat gattttgatt acgtcgatac gcgcacattt
    17161 ttacgcgccg tcgatgcggt actgccgcag ttaaagcccc tgtttgagaa atacgccgaa
    17221 gaaattgact ggcgtttgct ggccgctatt gcttatcagg aatcgcactg ggatgcacag
    17281 gccacttcac cgacgggtgt gcgcggcatg atgatgttaa ccaaaaatac cgcgcaaagc
    17341 ctcggcatta cggatcgtac cgatgccgaa cagagcatca gcggtggcgt gcgttatttg
    17401 caggatatga tgagtaaagt gccggaaagt gtgccggaga acgaacggat ctggtttgcc
    17461 ctcgctgcgt acaatatggg ctatgcgcat atgctggatg cccgcgccct gacggcaaaa
    17521 accaaaggga atcctgacag ttgggctgac gtaaaacagc gtctgccttt acttagccag
    17581 aaaccctatt acagcaagct gacttacggc tacgctcgtg ggcatgaagc ctacgcttat
    17641 gtcgaaaata ttcgtaagta tcagattagc ctggtgggtt atctgcaaga gaaagagaag
    17701 caggctacag aagcggcgat gcaactggcg caggattatc cggcggtatc gcctacggag
    17761 ttgggcaaag agaaatttcc ttttctctcg tttctttccc agtcgtcatc aaactatttg
    17821 acccattctc cctctctgct gttttccagg aaagggagtg aagagaaaca aaattaatcc
    17881 gtcgaggatt gcgctttttt ctgcgcttta atttcctggc ggcgcatgcg aaagaagtca
    17941 ctgagcaacg ccgcgcactc atccgccagt attccttccg taatttccac tcggtgattc
    18001 atacccggat gatgcagcac atccattaaa gatcccgcag cgccagtttt cgcgtcacgc
    18061 gcaccaaaga ccacgcgacc aatgcgactg tggatcatcg ctccggcaca cattacacat
    18121 ggttcaagcg tgacatacaa cgtggcgtcg atcagacgat aattttgcat caccagacca
    18181 ccctgccgca gggccatgat ttctgcatgt gcggtgggat catggcgacc aatcgggcgg
    18241 ttccagcctt cgccgattac ccgattgtta tgcactaata ccgcgccgac cggcacttcc
    18301 cgctcatccc aggcacgttt cgccagcgtc agcgcgtgac gcatccagta ttcgtggcta
    18361 aattcgactt cagacaaaaa gaaaactccg gttataaaag cgcggcgcat tatacacgga
    18421 cgctatgctt tactctattc cagttgctgg agttcaccgc gcggggttac tcgccaacga
    18481 tgctgacaga aataaagcag cggattgtcc tgattactgt cgctatagcc actgtacagc
    18541 cgcagcggcg tgccgatttt gcgctccagt tgtgcgacct tttcatgtcc cagacaacgc
    18601 atcgtcaata cccaaccacc atagccacgc tgaatttggc tggcgataag attaacccgc
    18661 ggcagccagg gcgtatcgaa ataaaccgct tcaaccagcg gctgcggaga gccggtaatc
    18721 aaccagatat cagcatcgga acttaacagg taggtggtta atcgctcctg aaccagcgga
    18781 aaggcggtaa cattgtcgcg aaaccagcgc acgaaatcgg cctgcaacgt ctgtaaacgt
    18841 gcttcgctgt gaccaaaagt gcacccccac agaagcagac tcatcggcca gcgtgccgca
    18901 cgacctttta tcaataacgc aatggctata atcggtaaca acggcaggac aagtaacgca
    18961 ttcagcggtt ggcgacgcag taaatagcgc agaaaactgc cgaacatatc ctgctgatgc
    19021 aatgttccat ctaagtcaaa aaacaccaca cgacgctcgt gagttgccaa accatactcc
    19081 tctggactga aacatcctga ttaattttcg ctactacata gcctaacaga tagatcatca
    19141 cttttccggc aacaatcgac cgcatcatgg ctaactggaa tttttaattc atggcaatta
    19201 gcggcaatgg aatataaaat tcactcgcgt gtgtctcata ttgcctgttg tcgccactat
    19261 ttcatcgcga ggaaagatat gaacggctta cttcgtatcc gtcagcgtta ccaggggctt
    19321 gcccagagtg ataaaaaact ggcggattat ctgctgctac aacctgatac ggcgcgccat
    19381 ttaagctctc agcaactggc caacgaagcc ggagtcagtc agtccagcgt cgtgaagttc
    19441 gcgcaaaaac tcggctataa aggttttccg gcgcttaagt tggcgttgag tgaagcgctg
    19501 gcaagccagc cggaatcgcc ctccgtgccc attcataacc aaatccgcgg tgatgatccg
    19561 ttacggctgg tcggcgaaaa actgattaaa gaaaatactg ccgctatgta cgcaacgcta
    19621 aacgttaata gtgaagagaa actgcatgaa tgcgtaacaa tgttgcgctc tgcgcggcgg
    19681 ataattctga ccggtattgg cgcttcgggt ctggtggcgc aaaactttgc ctggaagctg
    19741 atgaagattg gcttcaatgc tgccgcactg cgcgatatgc atgcgctact cgcaacagta
    19801 caggcgtcgt cccctgacga tctgttatta gccatttcct acaccggtgt acgacgcgag
    19861 ttaaacctgg cggcagatga gatgctgcga gtgggcggaa aagtgctggc gattaccggc
    19921 tttactccga atgccctgca acagcgtgct tctcattgcc tgtataccat tgccgaagaa
    19981 caagcgacaa acagtgcttc aatctctgct tgtcacgctc agggaatgtt aacggatttg
    20041 ctgttcattg cgctgattca gcaggatctg gaactggcac cagaacgtat tcgtcatagt
    20101 gaagcgctgg tgaaaaaact ggtctgagta aagaatgcgc gtataatgcc cgcccggttt
    20161 gtgttgtttt gagagtttcc ttatggcgct gttaatcact aaaaaatgca tcaattgtga
    20221 tatgtgtgaa atcaaataaa aacacattta ttttcatagt gatacagcaa tgtgttattg
    20281 aatgtgttgt tggatttatg gtgcaaaaaa attttgcata agttcagcaa cacaaagaac
    20341 accactttct ttaacgtcct ccgcgcccta aaggacgggg ctttacggcg caaaaggcct
    20401 gtgacataca acattaactt ttcagtcaac cgtagcacgt ccttgtatac gccgtgtctg
    20461 cggttttata taaccctggc tggaaacctc ttatacaaag tcgattcgcc aacatcatag
    20521 atgatcgcca ccttctggcg agaaactcct gatgcaatta gtcgcccggc ctgcgcccat
    20581 tattctggtg aaagtttggg acggcgaccg ccaattcgtc cctgtgcgcg agcagcttcc
    20641 agtcctgctt ttgttcgttc aacaatcagt tcacgttcca tttcagccag ggcacccatc
    20701 acatggaaga aaaagcgtcc cattggtgtg ctggtatcaa ttgaatcagt cagactacga
    20761 aagttgatgc ctcgttcgcg caactcctcc accagcacga caagatgccg catactgcgc
    20821 cccagccgat ccagcttcca gacaaccagt gtgtcgcctg ccgataatgt cctgagcagt
    20881 tttttcagcc ccggtctttc ggactttgta ccacttatct tgtcttcaaa aatcagctcg
    20941 catcctgcac agttcaacgc attacgttgt agatcggtat tctggtcatt tgttgatacg
    21001 cgtacatagc caataagcat ggtaaat
//
LOCUS       DADMGC010000069        20731 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.069, whole genome shotgun sequence.
ACCESSION   DADMGC010000069 DADMGC010000000
VERSION     DADMGC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.069"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..600)
                     /locus_tag="JNZ73_002435"
     CDS             complement(<1..600)
                     /locus_tag="JNZ73_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY8732685.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            1048..2019
                     /locus_tag="JNZ73_002436"
     CDS             1048..2019
                     /locus_tag="JNZ73_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8732686.1"
                     /translation="MLEKTINNAICALLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSE
                     LDGLFQQDATSISGKLRIDIPSGIAKNLLLPRLSEFLYQHPGIELELSSHNRPVDILH
                     DGFDCVIRTGALPEDGVIARPLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGGMLTVNSSENYLAAGLAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEHLD"
     gene            2068..3081
                     /gene="yhjD"
                     /locus_tag="JNZ73_002437"
     CDS             2068..3081
                     /gene="yhjD"
                     /locus_tag="JNZ73_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HAY8732687.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            3492..4814
                     /locus_tag="JNZ73_002438"
     CDS             3492..4814
                     /locus_tag="JNZ73_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAY8732688.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(4996..7056)
                     /locus_tag="JNZ73_002439"
     CDS             complement(4996..7056)
                     /locus_tag="JNZ73_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY8732689.1"
                     /translation="MSKAGKITAAISGVFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLHIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(7126..7893)
                     /gene="pdeH"
                     /locus_tag="JNZ73_002440"
     CDS             complement(7126..7893)
                     /gene="pdeH"
                     /locus_tag="JNZ73_002440"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HAY8732690.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            7906..9054
                     /locus_tag="JNZ73_002441"
     CDS             7906..9054
                     /locus_tag="JNZ73_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY8732691.1"
                     /translation="MFKSDAARTRQRLSARKGRTLPPGRGKFPHSTTESFRNTFWLKK
                     IMEHCFNMVDQQTTTAQTNANFLQIRFTTMSKKIAVIGECMIELSEKDADVKRGFGGD
                     TLNTSVYIARQVDPAALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGL
                     YYIETDSTGERTFYYWRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTS
                     REKLLSLLRECRAKGGKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDA
                     LWGQQPVEDVIARTHNAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTA
                     AGDSFSAGYLAVRLTGGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(9150..10646)
                     /locus_tag="JNZ73_002442"
     CDS             complement(9150..10646)
                     /locus_tag="JNZ73_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY8732692.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(10867..12153)
                     /locus_tag="JNZ73_002443"
     CDS             complement(10867..12153)
                     /locus_tag="JNZ73_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAY8732693.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFSSGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(12337..14325)
                     /gene="hmsP"
                     /locus_tag="JNZ73_002444"
     CDS             complement(12337..14325)
                     /gene="hmsP"
                     /locus_tag="JNZ73_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HAY8732694.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(14407..16155)
                     /gene="bcsC"
                     /locus_tag="JNZ73_002445"
                     /pseudo
     CDS             complement(14407..16155)
                     /gene="bcsC"
                     /locus_tag="JNZ73_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            complement(16483..16659)
                     /locus_tag="JNZ73_002446"
                     /pseudo
     CDS             complement(16483..16659)
                     /locus_tag="JNZ73_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            complement(16718..17824)
                     /gene="bcsZ"
                     /locus_tag="JNZ73_002447"
     CDS             complement(16718..17824)
                     /gene="bcsZ"
                     /locus_tag="JNZ73_002447"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HAY8732695.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(17831..20170)
                     /gene="bcsB"
                     /locus_tag="JNZ73_002448"
     CDS             complement(17831..20170)
                     /gene="bcsB"
                     /locus_tag="JNZ73_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAY8732696.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIREYGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(20181..>20731)
                     /locus_tag="JNZ73_002449"
     CDS             complement(20181..>20731)
                     /locus_tag="JNZ73_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732697.1"
                     /translation="ESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKIN
                     GQAQILEGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFAR
                     ADTWALWQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVS
                     FIPRRPERSETAQPSDQALAQQ"
BASE COUNT         4826 a         5750 c         5227 g         4928 t
ORIGIN      
        1 ggaggagata tttaacatca ttgccgcctg ggtgcgattt ttcacttcca gacggcgata
       61 cagggattcc agatgcgctt ttaccgttcc ggtactgata ttcagcgctc tgccgatctc
      121 cttatttgat tcgcccgctg ctaacatggt taaaatctcc cgctggcggg cgcttaacga
      181 tttgagatct ttaatgtcct tttccggcgt cgtccgccag tctccaggca gaaacatcat
      241 ccccatcgcc gcactattta ccgccaacgc aaatgtctcg acggttgaat cgcgaggcac
      301 aatggccagc acattaaaat ggataacttc ctgtaaccac cgtttattgc aatccgtcgc
      361 cgtgattaac accttaacct caggaaactg caccacggtt ttttgcagca accagtagca
      421 aaactcacca tcctgatcgc catcgagcat aactaaggct tcagggtaac tttccagctt
      481 ttgccataac tcgtctgcct gactgacccc ctgaatactc actcctggaa tacgctgctg
      541 taaactgatt ttcattccat gaataaatat tgactgcctg tcaaacatga ctatttgcat
      601 aactgaatct ccacctgaat acgttaaaaa gacttaagta gtggaagggt attacccgcg
      661 agaaaaaata agaattcgcc atttggcggt ggccattcta cagagatgac gcgtagaaaa
      721 tagttaccga tataaatagt tacagctaaa cgcctgaaat tacatgtcga gggcactatt
      781 taaaacaatt ttgaggattt ccttatattg gtggttagta cgcatgcaat taaaaatgaa
      841 attccgcgac tacaagccaa aataacaaat ggcaatgaga caaaaataag cacaaatagc
      901 caatacgtcc tctattcact ttaaagggaa tcactgaaaa atacgctctg tttaagggga
      961 ttcacctttc tcataaagct attccgccct tttcctgctg agaaatcgcc acattcgtca
     1021 tgacaacatt gtgaaacccg gcattagatg ttagaaaaaa caataaacaa tgcgatatgc
     1081 gcgttactgt ttcgctgtga acaacaatcg gtcaaagaaa tggataaaat tcacgcaatg
     1141 cagttgttca tcaaagtcgc ggagctggaa agtttttccc gcgcggcgga tttctttgct
     1201 ttgccaaagg gaagtgtttc gcgccagata caggcactgg aacatcaact tggcacccag
     1261 cttctccagc gcactacgcg acgggtcaaa ctcacgccag aaggcatgac ctattatcaa
     1321 cgggcaaaag atgtgttgag taatctcagc gaactggacg gtctgtttca acaggatgcc
     1381 accagtatca gcggtaaatt acgcatcgac atcccgtcag gaatcgcgaa aaacctgtta
     1441 ctgccgcgtc tgtcggaatt tctctatcag catccgggaa ttgagctgga actgagtagc
     1501 cataaccgtc cggtagatat tcttcatgat ggttttgatt gcgtgatacg cactggcgcg
     1561 ttaccggaag atggcgttat cgcccgtccc ctcggcaaac taaccatggt caactgtgcc
     1621 agtccgcact atctgacgcg ctttggttat ccgcaaagcc ccgatgatct gacttcacac
     1681 gcaatagtgc gttatacacc gcacctgggt gtacatccgt taggttttga ggttgccagc
     1741 gttaatggcg tccagtggtt taagtctggc ggcatgttga cggtaaacag tagcgaaaac
     1801 tatctcgccg ccggtcttgc cggtctgggg attattcaga tcccgcgcat tgccgtgcgc
     1861 gaagccctgc gtgccgggcg gcttattgaa gtgttgccag gctaccgtgc cgagccgctc
     1921 tccctttcgc tggtttaccc gcagcgtcgg gagctttccc ggcgtgtaaa cctgtttatg
     1981 cagtggctgg ctggcgtaat gaaagagcac ctggactgac cgactatact ttttaagaat
     2041 gaccacgaca gaaggacaaa agagcggatg acgcaggaaa acgagatcaa acgtcccacc
     2101 caggatctgg agcacgagcc gattaagcag ttggataata gcgagaaagg cggcaaagtt
     2161 agccaggcgc tggaaaccgt caccaccact gccgaaaaag tccaacgcca accggttatt
     2221 gcgcacctta ttcgcgcgac agaacgcttt aacgatcggc tgggtaacca gtttggtgcg
     2281 gctatcacct atttctcatt tttgtcgatg atcccgattt tgatggtgtc gtttgccgca
     2341 gggggctttg tgctggcctc ccatccgatg ttgctacagg atatcttcga caaaattctg
     2401 caaaacatca gcgatccgac actagccgcc acgttgaaaa acaccatcaa caccgccgtt
     2461 cagcagcgta cgactgtagg gcttgtcggc ctggcggtgg cactttattc cggcatcaac
     2521 tggatgggta acctgcgtga agcgattcgt gcccagtcgc gcgatgtctg ggaacgctcg
     2581 ccgcaagatc aggagaagtt ctgggtaaaa tatctgcgtg attttatttc actgattggt
     2641 ttgttgattg cgctgattgt gacgctttcg atcacctcgg ttgccggttc ggcgcagcaa
     2701 atgattatta gcgccctgca cctgaacagc attgagtggc tgaaaccgac gtggcgattg
     2761 attggcctgg cgatttccat cttcgccaac tatctgcttt tcttctggat cttctggcgg
     2821 ttgccgcgcc accgtccgcg caaaaaagcc ctgattcgcg gaacatttct ggctgctatt
     2881 ggttttgaag tgattaaaat cgtgatgacc tacaccctgc catcgctgat gaaatccccc
     2941 tctggcgcag catttggttc cgtgctgggg ctgatggcat ttttctactt cttcgcccgt
     3001 ttgacgctgt tttgcgcggc gtggattgcc accgccgaat ataaagacga cccgcgaatg
     3061 ccagggaaaa cgcagcctta aaataggccg gatgcggcgt aaacgcctta tccggcatac
     3121 gattttattc ggcctggctc cccgtaggcc ggataagatg cgccagcatc gcatccggct
     3181 ataatgcgca cataacctct tgaaactctt ccccagagcc tcttcagcca tctatttggg
     3241 agcaaacaat ttcactccaa ctcataaccc cagcatataa atccagttgg taacttttat
     3301 ttaacctgaa accagtttta tccactattt ataaaattat gtgaagcatt tcatagaaga
     3361 aaaatcactg gcctaaacat tatccccttt ttgcctggtt tttgaccatt tccgcgattt
     3421 gttacacatt gaaatatcac ttttgctgtg cgtaatatgg ctattcgtta gccaaaaaat
     3481 aagaaaagat tatgcaagca acagccacaa cactcgacca cgaacaagaa tacacgccga
     3541 tcaactcgcg taataaagtc cttgtcgcct ctctcattgg cacggccatt gagttcttcg
     3601 acttttacat ttacgccact gcggccgtta ttgtgtttcc gcatatcttc ttcccgcagg
     3661 gcgatccaac ggcagcaacg ctacagtcgc tcgccacctt cgccatcgcc ttcgtcgcgc
     3721 gccccattgg ctctgccgtt tttggtcatt ttggcgatcg cgttgggcgt aaagcgacgc
     3781 tggtcgcctc gttgctaacg atggggattt cgaccgtggt gattggtctg ctgccgggct
     3841 atgccacgat tggtattttc gccccactgc tgctggcgct ggctcgattt ggtcagggtc
     3901 tgggcttagg cggtgaatgg ggcggcgcgg cgctgctggc gactgaaaac gccccaccgc
     3961 gcaaacgtgc actgtatggc tccttcccac agctaggcgc accgattggc ttcttctttg
     4021 ccaacggcac tttcttgctg ctttcctggc tactgaccga tgagcagttt atgagctggg
     4081 gctggcgtgt gccatttatc ttctcggcgg tgctggtcat tatcggcctg tatgttcgcg
     4141 tgtcgctgca tgagtcgccg gtgtttgaga aagtcgctaa agcgaaaaaa caggtgaaga
     4201 tcccgctggg tacgctgctg accaaacatg ttcgcgtaac cgtactgggt acgttcatta
     4261 tgctggcaac ctatacgctg ttttacatca tgacggtcta ctctatgacc ttcagtaccg
     4321 ccgccgcgcc agttgggctt ggcctgccgc gtaacgaagt gttgtggatg ctgatgatgg
     4381 cagttattgg ttttggcgtg atggtgccag tcgccggatt actggctgat gcctttggtc
     4441 gccgtaaaag catggtgatc atcaccacgc tgatcatcct gttcgcgctg ttcgccttta
     4501 acccgctgct cggttctggc aacccgattc tggtttttgc cttcctgctg ctggggttaa
     4561 gtctgatggg gctgaccttc gggccgatgg gtgcgctgtt accagagctg tttccgacag
     4621 aagtgcgtta caccggagca tcgttctctt acaacgtatc gtcgattctc ggggcttccg
     4681 ttgcgccgta tatcgcagct tggttgcagg ctaactacgg gttaggtgcg gtggggttat
     4741 atctggcggc gatggctggc ttgacgttaa tcgccctgct gctgacccat gagacgcgac
     4801 atcagtcgtt gtaataagtg tgaaaagcct gatgcgctac gcttatcagg actgaatttc
     4861 catacaatat attgaattca caaggatttg taggccggat aaggcgttca cgccgcatcc
     4921 ggcatgaaca aagcacactt tgccaaaaat ctaaatctgt ttaattgccg gatgtcagac
     4981 atccggcaat tatcatcact tcttcatctg cgacaatatc gtccgacact gattagcctc
     5041 cccttcggaa ggtgagatca gtgccagtaa cgccgccgcc ggtgttacca gcgttgccag
     5101 tgccgcagca acagcaccac gggcaatcag cggtccggct ttcaccccag cctgcggatt
     5161 tttaaacgtc ccacgcacat acagcggcga acgcagtgtg ataatccgaa tccctttact
     5221 ctccggatca atagtcaaat ccagctgttc cgaagcaaaa cttgccgtgc cggtaacatt
     5281 aatcaacgcg ttctcagtat cgaaagcaaa aatctgcggg cgcgccacgc cgttggcaat
     5341 atgcagattc gccgccgcgc agttcacccg cacctcatcg tcaccaaata tcgcaccgac
     5401 aatgtagttg ccgacattca gcccaacaat ctccatcagg ttgcggctca ccagcccgtc
     5461 attcatcaac agtttcaggt tgccgttact gttgcctaaa agcgccgcca ccgagttacc
     5521 gctaccgcgt agttccgcgt caccgttcat ttcccccagc gtcttctgca tcagttccac
     5581 atcgggcatc agttctttca gtttcagtcg acgagcctga atatctgccc gcccctgcat
     5641 cggcttttta tcgccttcca gatgaatatt cgccgcaatg ctgccgcccg ccatgccaaa
     5701 tttcagcggt tgcaggcgca ggtcagcatt tttgaggatg atatgagtag aaagatcgct
     5761 aatcggcagg ctactgccat gctcaatgcg ccgccctttg aagcgaacat cggcatccat
     5821 aacgtcccat ttatcggttt cgaagcggtc ataaggcagc actttgcccg caggctgaac
     5881 gcttttttcg cccttcttct gttcagaccg tttcgacttt tctgcccctt tcccggaatc
     5941 aacgccaatc aacggtccca ggtccgccag ccgcaattgc cgcgactcga catcaccttc
     6001 cagttttggt cgtggctttc cggtggtgta gaccagagaa ccgtggatat cgctatcgcc
     6061 aattcgccca ttaaaaccgc gataatcaaa gaccgacgat ttttcagtgt cgattttcgc
     6121 taccagccga ccatccgttt caaacggcgg ggtatcgggc agcagaacgc ccgtcagttc
     6181 atagagatca cccagtgaat cgccagaaaa tttaagccgt aaatcgacac cgcccatctt
     6241 cattgggtca ttcacgacgc catcaaaagc aacacgggta ttacctgaac ggaaatcagc
     6301 ctgtaccgga aacggcgtcc cttcgccacg cagcgccagc ataccgccta tttttcccgt
     6361 acccgtgagc ggttcaccgt tataacgtcc ctgcgccttc aggccaaaaa tgtaatcgcc
     6421 caccttttct ttatccgctt tacctttcga tccagtaact tcgctgaatg gcagcggctt
     6481 gcctaacgga tcaacaaaaa tttccagatc cgctttgctt actttgtcat caatggcgat
     6541 ccgcccttga tcgaaaagaa tattatccag ccgaaacgac catgccgacg gctttgcatt
     6601 cgcgtctttg ttgtcatcgt tggcaagatt aaacgtccag ttattgttct tttcagagag
     6661 gcgaatcagg cgcgcgtcgg gcttttcgag cttgatccac ggcagccaga ccgttttggt
     6721 cagcagcgcc agcggtgcca gcgttgcctc tactcgcggc aaatgcacca tcgtgacttc
     6781 gggaatatcc ggtggattac caagaatgat gtcttctgca tgtacatgag gccacggcac
     6841 ccagctgcgc cagccagttt cttgtttttg ccgctcccac accacgccca gatcgccacg
     6901 gatagcgaac ggacgattca actccgcaga gactttctgg ttgatggtcg gtttgagtcg
     6961 attccagtca aaagttgcaa tcaaaatgat cgccacgaca atcaacaaca agaaaacccc
     7021 tgaaatcgca gcggttattt tgcctgcctt gctcatcgtt cgcttcctca tattcttcct
     7081 gtgccagtcc taaagatagt ccagcccggc ggaaaatgag gcagcttata gcgccagaac
     7141 cgccgtattc agcgtttcca tcggtgccgg gcgtgaaaga aaccagcctt gtgcggcgaa
     7201 tgcgggcgag ttctgaacat cacgccactc ttccggtgtt tctacgccct cgacaatcac
     7261 cccgcgacaa tagcgattca ttagatgtaa aagctgagaa aagagtgtgc ggccttccgg
     7321 cgactgacgc agcatcacaa acagttctcg cgcgattttg atgtagtcat aacgcacttc
     7381 acttagtgca gagaaatttg ccatcccggt accaaaatca tccagccaca gcgggccaaa
     7441 ttcacacatc gaggcaaagg ttgaatcttt cggcagacgg atatgctcca ccagttcgaa
     7501 acgcagccag ggaagacgct caatctggcg caggattttt ggttgctgac gcagggcgat
     7561 gagcgtaggg ccatcaatat tgaccgatgc cagcaggccg tgctctacaa agaagtcggc
     7621 tttttgcgcc agcaaatcaa tctgctcttt cacaacctcc atccgatggc tgacggtgat
     7681 ttcagtaaaa tagcgatccg gcggcaggcg ttgcgaaggg ttcaagggat gcgtgaccac
     7741 cgttaatagc tccacggcca ttaaccgccc gcatgtttga tagatcggct gccaggtgta
     7801 agcacgctca cactgcaacc aaaaacgccg ttcctgcaag ctctcgatgc ttgcttcagg
     7861 gttgcttatt cgctggataa cctgccttat catcaaagat gtcctgtgtt taaaagtgat
     7921 gctgcccgga ctcgacaaag attatcggcg cgtaagggca gaactttacc gcctggccgg
     7981 ggtaaatttc ctcactccac aacggaatct ttcaggaata cgttctggct caaaaaaata
     8041 atggaacact gttttaatat ggttgaccag caaaccacca cagcgcaaac taacgctaat
     8101 tttttacaga tcaggttcac gactatgtcc aaaaagattg ccgtgattgg cgaatgcatg
     8161 attgagcttt ccgagaaaga cgcggacgtt aagcgcggtt tcggcggcga taccctgaac
     8221 acttccgtct atatcgcccg tcaggtcgat cctgcggcat taaccgttca ttacgtaacg
     8281 gcgctgggaa cggacagttt tagccagcag atgctggacg cctggcacgg cgagaacgtt
     8341 gatacttccc tgacccaacg gatggaaaac cgtcttccgg gcctttacta cattgaaacc
     8401 gacagcaccg gcgaacgtac gttctactac tggcgtaacg aagccgccgc caaattctgg
     8461 ctggcgagcg agcagtctgc ggcgatttgc gaagagctgg cgaatttcga ttatctctac
     8521 ctgagcggga ttagcctggc gatcttaagc ccgaccagcc gcgaaaagct gctttccctg
     8581 ctgcgcgaat gccgcgccaa aggcggaaaa gtgattttcg acaataacta tcgtccgcgc
     8641 ctgtgggcca gcaaagaaga gacacagcag gtgtaccagc aaatgctgga atgcacggat
     8701 atcgccttcc tgacgctgga cgacgaagac gcgctgtggg gtcaacagcc ggtggaagac
     8761 gtcattgcgc gcacccataa cgcgggcgtg aaagaagtgg tggtgaaacg cggggcggat
     8821 tcttgcctgg tgtccattgc tggtgaagcg ttagttgatg ttccggcggt gaaactgccg
     8881 aaagaaaaag tgatcgatac caccgcagct ggcgactctt tcagcgccgg ttatctggcg
     8941 gtacgtttga cgggcggcag tgcggaaaac gcggcgaaac gcgggcacct gaccgcaagt
     9001 accgttattc agtatcgcgg cgcgattatc ccgcgtgagg cgatgccagc gtaaacgcag
     9061 catatgcaaa aggctggata aagtgttacc cctctatcca gctcatccat aaatacctga
     9121 tggttgtgct aactcatcac catcaggcat tactgtgcag gtggaatatc cgtcacttca
     9181 ggatgtacgt catccgtggc gacagaggtg gtcgcagcgg cggtagatgg ggccatgatt
     9241 tgatcccagg ccgcctgcaa cgctttcatg ttaaattccg gctcgccttt cggctgcagc
     9301 agtattaacg ccatatcatt cgacaactgc tgacgcagat cctgatttaa catctccaca
     9361 gtcaggctgt taaggaaatc ctgccgcagt ttctgatact gctccggcgc gatatcgacg
     9421 acctgatttt gcaacgaacg catccgctgg cccatcagaa tatcggtatc agcccgtgca
     9481 taggcggcaa acagtttctg cagctccagt ttcttttgcg ccactaacgc attgaactct
     9541 tcttccggca gacctttatc gcgaaccttc gccagttcac gcgccaccag attaaggttg
     9601 ctgttcagct tgtcgtttgg tgattcgatg ttgatggcac actgcgcacg cagatacagc
     9661 acacggcagt caaatccaag accgatgtct ttgctgtcac tggcgcttaa cgcttgctga
     9721 acatgccaga acagcgcctc gcgggccagg tccgcacgcc agtagcgcag cagtgcggct
     9781 gattcacgaa tcggctgcca cggcgtatcc cacatgatag ataaccggtc ctgacgcacc
     9841 gcgtcagtca taatactcac cgcttccgca cgcagcggag aaagcgtcgg caccggagct
     9901 ggcgtttcac gtttgccttt cagttcgcca aacgttttgt tgatttggtc gacaaccgag
     9961 cgcgcatcca cgtttcccac caccagtagc gtcattgcat ccggggtgta ccatttctga
    10021 tagaaatctt taattttttc cgcttcaacg ggttgtttca gcggatcggc aggatcgtga
    10081 cctaacaagg ttgacccttt cagacgatag cgccaccagc cctctttagt atcggcaggc
    10141 caggttgcca ccatgtcctg actttgcagc gcgtggttga tggtttctgg tgtgatggtc
    10201 aatttgccag tggcatttgc cagataagag agcgcttctt tcagcaaatc gttacggtta
    10261 ttgggcaaac tcagattaaa cagcgtggtg tcataagaga caattaccgg cggcatcggg
    10321 cgtttagggt cgatcccctg ctgccacaat gaacgcgcct gtgctgcgtc aaggccaccg
    10381 ctttgcgtta gcgcaatacg agggatggcg tgactgtaac cgctctgttg tgtactttcg
    10441 gcgagcgaac cggtattaac cagcaggcga atttcaacac gatcgctggg acgctggggg
    10501 gtggtcagca cttgccactg taaaccgttg gaaagcgtcc cctgttgcca tgctggatca
    10561 ggctggagcg catctgcctg cacatagcca gcagtggcca tcatcagcaa accgcccgct
    10621 aaaagtcgaa tttttatgcc ctgcatgtga acccctgatc aactatcctg gtaataaaaa
    10681 aactgcccgt tgtcggtcag tctaaatgac gttaaaaaca cttcgtgtta gaccgcaaga
    10741 acatgaaaat gtcacggaag aagtgaaata aacctgaact cacccaggtg gtaagttcag
    10801 gcacaggggt caattatgcg caaacacccg cactcgggga agggagtgcg ggcataagtg
    10861 gtgagattaa gaggataatt cgtgcgtttt gccatccggc gcacgattat tcagcacatc
    10921 gtccagtttt ttgtggtcca gttctttcac ccacttagca acgacaatgg tcgctacgcc
    10981 gttaccgacc aggttagtta gcgcacgagc ttctgacata aagcggtcga taccgaggat
    11041 cagcgccaga cccgctaccg gcaaatggcc caccgcagaa agcgtcgccg ccagcacgat
    11101 aaagccacta cccgttaccc ctgccgcccc tttagaagaa agcagcaaca cgattaacag
    11161 cgtgatttgg tggacgatat ccatctgact gttagtggcc tgggcgataa acaccgccgc
    11221 cattgtcagg tatatcgatg tgccatcaag gttaaacgag tagcctgtcg ggatgaccag
    11281 ccccaccacc gatttacggc agccgagttt ctccatcttg tcgagcatac gcggcagcgc
    11341 cgactcggaa gatgaagtcc ccagtacaat cagcagttct tcacggatgt agcggataaa
    11401 tttgaagata ctgaaaccag tcgctttagc gattgaaccc aataccagca ccacaaacag
    11461 gatacaggta atgtagaaac agataatcag ctgccccagt tgcaccagtg tgccgacgcc
    11521 gtatttaccg atggtaaacg ccattgcccc gaacgcacca ataggtgcca gacgcatgat
    11581 catattgatg atgccgaaga tgacctgcga gaaactttcg atcacgttaa aaatcagttg
    11641 gcctttgctg cccagacggt ggagcgcaaa accaaacagt acggcaaaca gcagcacctg
    11701 cagaatgtta ccgctggaaa atgcgccaat gacgctcgcc gggatgacat ccataatgaa
    11761 ggcgacaatg ccctggtctt tcgcctgatc ggcgtaaacc gctaccgctt tcgcatcaag
    11821 cgttgccgga tcgacgttca ttccggcacc aggctgcacg acgttaacga tgataagacc
    11881 aataatcagc gcgatggtac tgacaatttc aaagtaaagc agtgcgactg cgccggtacg
    11941 accgaccgcc ttcatgcttt ccatgcccgc aatgcccgtt acgacggtac aaaagatgac
    12001 aggagcgatg atcatcttaa tgagcttaac gaagccgtcg ccaagcggtt tcatttgctc
    12061 gcctatttca ggatagaaat ggccaaggag aataccaatg gctatcgctg tcaggacctg
    12121 aaagtaaagg cttttaaaca gagaggtttt catagggtgt cctttagtaa aaccacaggt
    12181 cttgtaaggt tatgggggta cctgcggcct taaaataaca cccagacaac atcacagaaa
    12241 tgtacctgga tcataattga aacaaaaagg ttaaaaagtt tgagctggct cgcacaaacc
    12301 agcacttttt aaagttttgt aatcagtttg gggtagctac ttttcttcca ggtaactctc
    12361 ttcgaagatt tcaataggga gtgggcgagc aaaaaggaag ccctgggcaa taccaacgcc
    12421 cgcttttgcc agccagtctc gttgtgcttc agtctccacg ccttcggcaa tcatttgtaa
    12481 gttcaggctc tgcgccagca tgataattgc agcaatcatg ctgctatctt ccggcaagcc
    12541 ttcaacaaac attttgtcga ttttcagtac gtcgattggc aacgatttca tatgctgcag
    12601 ctgacgcagc cctgcgtagc ccatgccgaa atcatccagc gccacccgaa ctccggcatt
    12661 gcgcagcgga cggaggattg ccaccgcagc atgagggtcg tcaatacgtc ggctttctgt
    12721 cacttccaga atcagtgttc ccggctgaat gcgatagcgg gttaacagtt ccagcatatc
    12781 cgccaccata ttcgggtgca tcagttgcag cgcagagagg tttaccgaca agggcagcat
    12841 aatgccgcgc tcttgccagg ctgcaagcaa tcgacaggac tcttccagca cccagtgacc
    12901 gacggtaacc atcagcccac agcactcaat gcgatcgatt aagccatccg gcaggtccca
    12961 actgccatcc ggttgctgga tacgcagtaa cacttccgca ctgaccagtt taccgctggt
    13021 catctcgacc tgtggctgta accaaattgc aaactgatga ttttccagtg cattaaggat
    13081 atcgctctct tccgtcaacc gcttctgggc tgcttccatc tgctgcggat caaagaactg
    13141 aatctgattc ttgcctttat ggcgagcggt aaatgccgca gaaatagcgc gactgtaaag
    13201 ctgttcggcg gtgagatcgc cgtagaacat cgccacgcca atgctacagt gcggacggag
    13261 ttgaatacgt tcaatcggca ggcgctcgct catgatagtg agtacttgct gacccaaagt
    13321 gattgcgtgc cacggttcct gtacaccgtt ggcaatgaca gcaaagtcat aaccgctaat
    13381 ctgcgcgagg atcatacgtg gcgacagtac cgatttgagt ttttccacca gcgtcagcag
    13441 cagaatttct cgttgcgcct ctttcagcac gcccgcagta tcacgcaggg tttcacaggt
    13501 gatgatcatc agcgcggtgg tttgtttacg cgcgacaacc tgctccagca tctccatcag
    13561 caaggctttg ttcggcaaat ccgacaccgg gaagcgcatc gcattttcgt tctgttcttc
    13621 ataatggcgc tgcagcaatt gctggttgag gttgtaactg cgcaccaaca taccgatttc
    13681 atcgtcctga tgcagacgcg gtaatgccag ttggtgacca acaagctcct gggctgggat
    13741 ggcgttaagt tcgcgagcaa tattgcgtaa cggatgcaaa atcaggcggt taatgcacca
    13801 gctgatggcg acggtcagga taagcgacaa aagtaagtaa atggtcacta acgttgagag
    13861 agtgctcatc acgaacttat acatacggaa ggaatccgcc tgtagcacca gataggctat
    13921 tggctgcgga tttgccggac gttcgagcga gtagacgccc agcgagattt gaaccggtag
    13981 ctcaaacagg cgagtaacca ttaccggcac cggacgctct ggaataaaac ttttacgcag
    14041 cgcctggaac tggttaggca gcactacatc ggcacggctg accacgcctg ccggtttaat
    14101 gctggcaaga atagcttccg cttcgggaat atcgcctttc aaaatagctg aagataaggg
    14161 ttcgcggaca gagcgagcaa tgctttccag ttgcgtagcc gtgttatagc gattctgctg
    14221 gaccagatgg aacagcaaaa cggtgcaaaa aataaaaacg aacaccagga caacggctgc
    14281 caccattgcc atctgcttga ttgttaacga gcgacttacg cgcaaattgt ctccccccgg
    14341 aaatcccaga ttgccgcccg agtatacccg atcgcagcgg cattaagaga ggcgctatct
    14401 gaaaacttac cagtcggcgt aaggtatcag cggctgcggc ggtaaatcca tgtcaccctg
    14461 ccatccggcg gcggaataac gtacgtagag caggaaatgg ctgggtgcgt aatctttcgc
    14521 ctgctggata tcaattgccg tgccaacaaa ccagttggaa gtaacacgtc gttcaagtaa
    14581 tgcccgcgcc gtgtagccga agccctgact gctgccgcca tcgttggatt gccgcgcagc
    14641 ttcttcctgc cagtcggtcg ggatcagatt catcagcgga taacgcggca tggttttggt
    14701 gcgtgaatgc gaccacgagc cagacgcccc cagctcccac gaccagtttt ccgtgcgctc
    14761 ccgccacatc accggtatgg caaacgacag gtattcctgc ggactgtagt aaccgccctg
    14821 accgagtgag tagccactca gatctttgtc gtaatgccag atcatgttgt tcaggccgat
    14881 tgtgacgcgg cgattgttct ggttaatgac cttatagtaa tagcccgtca tccagcgcac
    14941 gcgccagtta tcttcgacat ttttaccggt taactggtcg ccactaagcg atgcccagac
    15001 gccgtttact tcacctttat cgtagctcag acttagcccc acaccgtcgg cacgtacgcc
    15061 accccatttt ttcccggtat tgctcgggga gtctttttgc ccaccaaagg ccagcaaaga
    15121 actggagatg ggccgacggt gggcgttaac ggtgtaaccc agcggcccga tatcatcgct
    15181 gtaactgatg ccgccgacca catccaccac gttgaagccc atcggcgtgg taccgatatc
    15241 ccagctccaa acgtcatttc gccagccgac cgccacgctg gcaccggaat ccgactggct
    15301 gcggttgccg ctacagtcct gtaatgtaca ggtgccccag ttgtcatccc atttgccatc
    15361 ggcattagtg gagaaactgc cgacgttcat attgacgaaa tcactgcgaa agaacatccg
    15421 cccgtcagaa tacggcgcat ccacctgcaa catggtagtg tgcgctttca gatcggagta
    15481 accaccggtg ccgctcgaac cccagtaatc gtgctcaagg gtgacgttaa gatcctgctg
    15541 gcgatagagg tccgccgcat cgctgcgcac gccgcgtttc agccagtcat ctttctcgtc
    15601 gttacgggtc agtcgggtaa aggtgtcgtt atcctgcgga cgcgtcgtag tcacaccgga
    15661 tgcgaccatg gcgtctttgt aggtttccag cgcctgcttt ggatcgcccg cctgcgcttc
    15721 aaacttcgca ccgtcgcgca gcaccatcgc gctttccatc gacggtggct gagattttgc
    15781 ctgcgggatc aacttattaa acgtctgctg cgccgctgcg gtatctccaa gctgcgcctg
    15841 tgccagcgcc acgcgccgct gagtgttcag cgaggtgtta tcggtggcag gcagtttcgc
    15901 cagctggcta cgtgccgccg ctttgtcacc ggcagcaata tccacttccg tcagaccaag
    15961 aatggcatcg gcgttagttg gctcccgcgt caggacattc tgatatgcag tgcgggcggc
    16021 ggtgtaatca cgtcgttgtt acgcccagtc agccagcgtg aggtcaatac gcgtggaagg
    16081 tggttgctga cgcagcatcg cttccgcttc agcctctttg ccgctttctc gcaggcgatt
    16141 agcggtttcc agcacctgat cgctttgcag tcgattaacc agctcctgaa tattgctgtt
    16201 ccactgcgcg cgcggcaggc tgttgatatg cgccagcgcc gctctgtcct ggttgtgacc
    16261 tgagaggtac agcccgtaag cgtaaacctg ctccgggttg ttcggcttct gctgcgccag
    16321 attgcgcatt aacgtatcgg cctggctgcg ttgtccggcc tgccagagat cctgcgaaag
    16381 tcggtaagta atccatacgc tgccggggtc cagcgccagt cgttgccgct gaagtgctgc
    16441 cgcctgcgcc catttgccct ggttttccag tacctctgcc tgctgtgcca gacggtcgtt
    16501 ttgcaggctg cgttcgatat catcaatgct gcgccgctgg ctggcagaga gcgaggcgat
    16561 aaacgcttca gctttttctg gcgactgctg gcggtaaata ttcgccagcc cgcgcacggc
    16621 attagtgttg ccgctgtcca tccgcagggt ctgctgatgg ttggtgctgc ctcgaccatc
    16681 ggcatgacgg ccagaccgag ggaaagcgtg aatatgttta gtgtgaattt gcgcattcct
    16741 ggccccagtc aggtaataac tcaccttttg tcgagaagcg gaaacggtgt tgatcccagc
    16801 cttgtccaaa cagggtcagc acatagttgt aataggtatc gctgccggga aagttatcgg
    16861 ccacgcgctg gcgctgaact gcctgcgcat cgcggttttg taaaaaaggc agcatggcgg
    16921 cagaaaaacc gaccggtcct ttaccctgcg ctttccccgt agccacatcc actttttccg
    16981 gcggataacc gtttttctca gtgaatgtcg ccatcggttt aaaccggttg agcatccgcg
    17041 ctttttgcgg atcgctgtca ggcatcatgc ctacccacat gtaaacgcgg atagcgtcgt
    17101 agctgctgat caatgttttt tcggctttta gctgccagcc tttgtctttc tcatagcgca
    17161 cccagtctgg cgaaaagcct ttcggggcgg tttccagcaa taaatgttga ttggtttcgc
    17221 gcagcgtggt ccacggcgcg ccaaagcggg tgaaatactg cgccagcgtc ggcggcatgt
    17281 agctggggtt aaaacgccag ctgttatcct cagcaaaacc cactttgccc ggtaacaaca
    17341 tggaacccag cccaggcacc gtcaccactt cctcccgcgc gatacgtttt agcaacgcgc
    17401 tgccgatgtc ggtataacgc tgctctttcc acaaacgccc cgcctccagc aacgaccagg
    17461 ccatccagac atcaccatcg gaggccgaat tgctgtccag cacttcccac ttactgttct
    17521 ctttcttgcc ccacagccag gcgggcaaac gttcttttaa agaaccctga gcgagattgt
    17581 tctgcgtcca gtcgagaata ttatcgaaag ctgcacggtc gttagccgcc agggcaaaga
    17641 acataccgta actttgccct tcggaggtgg tgattttgcg cgcgtcgctg gggtcgatga
    17701 cgcgcccttc ctgactgatg taatcctttt taaactgctc ccaggcaggc caggtacagg
    17761 ctgcctgaac actaaaggca gccaacaaca gcatcgtcac gattccacta cgcaacacat
    17821 tcatcttcaa ttactcgtta tccgggttaa gacgacgacg actaataatt cgcagcagac
    17881 gccacagtac ccatgccagc aatatcacac tgatagccgc cagcaccgcc agcagaatcg
    17941 gatggtttgc cagcgcatac cacaagcgct cgaaccacgg cagatgacct acgtaataaa
    18001 cgtcgccaac acgtaggctg ttgataccgt actcgcggat caccgcgacc gaaccgaaca
    18061 tggtggcgcg tttgccgcta tcgttcaccg catcgttaag catttcataa ccgcgtgggc
    18121 tatctgccaa cagcgcaatc acgctgcgct ggtcgttata cggcgactgg aagccaatca
    18181 ccgccgccat cgcaccggaa gaggtcagcg ttgactgggt ttctgccgcg cgatcgctct
    18241 cgtccggcac aatgccgggg aacggggtct ggcgcatcgg tgttttcacc cagctttcgg
    18301 tcgcctgcac caataggtcg atctgcttat cgtctttcag tttgtccggg ataccaccga
    18361 tgatcatgat gtcggcatct ttgccctgaa tggtgctgcc atcatcggtc accgtcagat
    18421 taatcgccgg gaagcccgtc tgtgcgccga taaaaccaac agtattcagc aacgtttcca
    18481 tctgtgcttc gttaggcgct ttcggcatca cggtgatggt ttgcgacaga tccgccatcc
    18541 ggctgaatgg gaagcccgcg ttagcaaagg cgcgtagatc cggcatcgga atgaagtggt
    18601 aatacttcga gaagtcgatg gtggaatcgt caccaatcac cacatgattc tgcaccggct
    18661 ggaaggtaat acagttatct accgaaccgc ctggcatcgg gttcatatac tcaaagtcga
    18721 agcgcagctg gttggtcgcg cccagtttca gcgccggaat agagacatct gttttgccat
    18781 ccagcaaacc ttgtaatacc ggaatacgca gcagcagacg gttcgcctcc tgtttgctgc
    18841 tcaggttgaa ggattgcagg aactggttat tcaggctgat atccatccgc gaactgtctt
    18901 tcaccggcgg catggtgtag cggtaattaa tatccatatc aatgccggta ctgcgcatca
    18961 ggtagagatc cggcggcagg tttagcgaaa cgttaatcgc tgctggctca aggccgctgg
    19021 attgtaactg ttcttcatag gttttcagtt cgccaaatgt gaccggacga tcggtacgta
    19081 cccagttcgg cgcatcgtac ggcttacgcg gtagcagcgg tttcacttca ttcactacca
    19141 cgctttcacc acggaacaga atgttaccct gagcgatacc tttcgctgcc tgcaacaggt
    19201 ctttgtcgtc acgaccaaac accaccagca gtttgacgta aggattctgc ggatggttaa
    19261 tcatctcaat caccggggct tttaccgccg gatgatcgcg caggaagtcc ggccgtttgt
    19321 cgttggttgc aaagacaatg gcattgcgat ccggcaattg gttatagagc accgggaagt
    19381 tctgcccacg ccagccagaa cgcgaaccaa accacgaggc gacaatggca gatgcttgtt
    19441 gcagcccaac atccggcgca cccgcaaaga ccatcggcaa ggtgttagta cggttatcgc
    19501 gcgggtcaaa gaatggcacc gggaagtgtg acaggtcatt cttcacattc agggtctgat
    19561 aggtcagatc cagtccactg ctccgcccaa catccagcca aagcgtggtg ctggccgggt
    19621 tttcgcacac gtcctgataa tggccgacaa actccagccg cacacggttg aagtcggtaa
    19681 taaacagtgg attaatgggc atttgcgcca gcgttttttt acccaactgt tctttggtca
    19741 ctggcagcac gcccatcagc tcatcattga gataaacctt taactgcgac tggacaggca
    19801 gtaacgatgg cgatggcgtg tattcgaggt tgagcatcgc cttcgtcacc acttcatcgc
    19861 tgcgcatacc aaactcaatg ctgccgttcg ggttaatacc acgtagcacc atgctgcccg
    19921 gcggcggcgc gatttgcgca aaggtcagct tcacatcacg cgaaggaccg ttctgcgcca
    19981 cgatgggggc atcagctccc tgctcgcctg acatcacttg ccccacctgc ggattttgtt
    20041 ccgtctgggc ggctacagct ggctcagcat tgatcagtgg ttgcgttgct ggcgtcgcct
    20101 gcgtcatgaa agaggggaac gcactcatcc ccatagccac tgcacaaatc cagaatagtt
    20161 ttcttttcat cgcgttatca tcattgttga gccaaagcct gatccgatgg ttgtgccgtt
    20221 tcgctccgct ccgggcggcg cggaataaac gatacaaccc aggaaaccag agaagtcagc
    20281 acacggaata tgcccttcac cgaagaaggc gcaaactccg ccagatggcg gtagccacgg
    20341 aagccgagct tcagaatatc cagcagactt tccagcggct tatcttccgg gtagctgtcc
    20401 tgccagagcg cccatgtatc cgcacgggca aacgtacact gcacaaaatc gatatgttgc
    20461 tgggtggtga gcggcattaa tttcagccca acttcattac ccatcacgcg cgccacctgg
    20521 gtcgggaaga cgtattcctg ctgaccgcgt ttaagcaaca gattcacttt ctgcccttcc
    20581 agaatctgcg cctgaccgtt gatcttgatc cccaaaccac cgtcggagaa atcctgaacg
    20641 gtacacgaga agaggtgacc atcttcgcgg gcaattgccg cgggcatcgt catctccacg
    20701 cggtgcgatc ggcgtacctg tttgctttct a
//
LOCUS       DADMGC010000070        20691 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.070, whole genome shotgun sequence.
ACCESSION   DADMGC010000070 DADMGC010000000
VERSION     DADMGC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.070"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JNZ73_002450"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JNZ73_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAY8732698.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMLRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQSEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JNZ73_002451"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JNZ73_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HAY8732699.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JNZ73_002452"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JNZ73_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HAY8732700.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JNZ73_002453"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JNZ73_002453"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HAY8732701.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGRVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="JNZ73_002454"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="JNZ73_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HAY8732702.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGTRVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="JNZ73_002455"
     CDS             complement(8217..9110)
                     /locus_tag="JNZ73_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HAY8732703.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="JNZ73_002456"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="JNZ73_002456"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAY8732704.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLVEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /locus_tag="JNZ73_002457"
     CDS             complement(10007..10189)
                     /locus_tag="JNZ73_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HAY8732705.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="JNZ73_002458"
     CDS             complement(10241..11473)
                     /locus_tag="JNZ73_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAY8732706.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="JNZ73_002459"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="JNZ73_002459"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HAY8732707.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="JNZ73_002460"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="JNZ73_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HAY8732708.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16497)
                     /locus_tag="JNZ73_002461"
                     /pseudo
     CDS             complement(14278..16497)
                     /locus_tag="JNZ73_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16704..16988)
                     /gene="ihfB"
                     /locus_tag="JNZ73_002462"
     CDS             complement(16704..16988)
                     /gene="ihfB"
                     /locus_tag="JNZ73_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HAY8732709.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17148..18821)
                     /gene="rpsA"
                     /locus_tag="JNZ73_002463"
     CDS             complement(17148..18821)
                     /gene="rpsA"
                     /locus_tag="JNZ73_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAY8732710.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18932..19615)
                     /gene="cmk"
                     /locus_tag="JNZ73_002464"
     CDS             complement(18932..19615)
                     /gene="cmk"
                     /locus_tag="JNZ73_002464"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAY8732711.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19788..20552)
                     /locus_tag="JNZ73_002465"
     CDS             complement(19788..20552)
                     /locus_tag="JNZ73_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY8732712.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4971 a         5718 c         5025 g         4977 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttcctg gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg actgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc agcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt gcttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactctg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgctg gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtacca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ctgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc caccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcctcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gactcggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaccg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccagaccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatacgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcacc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcaatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg ataataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgac cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggctatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagg ccagtcctgt gtcatagaga atcgctttgc cgtttctggc
    15001 tatcaccatt gccagccctt gcccgacatc aagcatgtac agctgccact cgtcaggtcg
    15061 aggtttttgc cacagcggcc agcacatcag caagcctcca gccacacaca ttactggcaa
    15121 cgttcgccag gcgtttaatc gccataccac cagtaagaac catggggaaa atgatagcca
    15181 ttgccaacat tcaacaatgt tgatccaccc ttccggcaaa ctctttaacc cctgttccag
    15241 gattaacggc ccgctaaaat gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa
    15301 tgtcaccaag ggaattgcaa atagatttgc aataaacgag gtcagactaa tgccatgaaa
    15361 tatgacgatt tgcatgggca taagcaggag tgtgattccc agttgcagat ggatgaggga
    15421 aacaactgca cgcaataccg gcggcagttg ccactcagga cagggaaacc actgatacca
    15481 aaaaatcagt gccgcgaccg cagcggcaga gagccataaa ctttgcgaga gaatggcaac
    15541 aggatccatc agcaaaattg ccgccagaca acatatccat acatcccagc cactccactg
    15601 tcgcccactt aacttaagca ttccccacgt agcaagcgcc accacggtac gcaatgcagg
    15661 aggttgcatt cccgtcaacc aggcataaaa agcagcacag cagattccgc caattaatgg
    15721 tatttgccag tggatccagc gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc
    15781 agccagcaac gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc
    15841 agtatcgcgc atgatatttt tgatttcttt ggggacggat aaccgttccc ccatacctaa
    15901 accaagaata accgcattcc acggataggg ttgcagcgtt gtttgtagtg acgccagata
    15961 ctgtgcacgc aggctacaat tcggttcaat gacacttgcc tgcagaaaac ggccggtgag
    16021 cggctgatgc tgggcaatgg cataacgctg gctatcaaag ccgccatcat taagttggcc
    16081 atgaactgca cgaactttga gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg
    16141 aagttattcg ccatacagca caaggcctgg cgcagggaat atacgtcgac cttgtagata
    16201 agtaatttga ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa caattgcatc
    16261 ctgcgtcgcg ccagttaagg tttctcctgc ccacaaaatt tgctttgctg ataatatgcc
    16321 ccaaacgaaa aacagcaacg tcagcgcgat ataacggacg gttttaacag ggataaatgc
    16381 cagtacgcag gcgaagagag tcagaaacgc aagggttaat gtcctaggca attggggcaa
    16441 aatcagcaac ggaaaaattc cgcaaattat gcatacgccg actgtcgtta ttttcatgcc
    16501 cccctccctg tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt gggaattttg
    16561 cgttccccga gacacattca gaagaattta acgaaagggt gaaaactgct caaaactctg
    16621 cgaggcagct tccagttcct gattagagaa ttgaaaaaag cacccgacag gtgcttttct
    16681 cgcgttcaag tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg cagttcttta
    16741 ccaggtttaa agtgaggaac gtattttcct tccagttcta ctttatcgcc agtcttcgga
    16801 ttacgtccgg tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc gcggatttca
    16861 atacgctcgc cctgcgcaag agtcgaggcc atatgctcca gcatctcttt tactgcatct
    16921 tcaaccgtct tggcgggaat gtgcgattgc tgggtggcaa gtctttctat caattctgac
    16981 ttggtcatga ttcctccggt tccttaaagg caaattaagc ggctgctgtt gctgcattga
    17041 ttaagggcgg ccgtagccgc ccttagtgct tgattacagg acgaaacctg caatctgtca
    17101 agtaaactca acaaacttcg gaataaaaat cccgaagagt cagagaatta ctcgccttta
    17161 gctgctttga aagcttcagc cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta
    17221 acagttgcga ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga caggctgatt
    17281 gcgcggtttt tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga
    17341 accagggtag cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag gtaaccttca
    17401 acgccgtcag ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt
    17461 acgatagcgc ctttcttgtt cagagcaacc cagttgttga acggatcttc tgcgagctgt
    17521 ttaacgccca gggagatacg ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt
    17581 tcgtcgcctt ttttgtattc acgaactgct tcttcgcctg caacgttcca ggagatgtca
    17641 gacaggtgaa ccaggccgtc gatgccgccg tccaggccga tgaagatacc gaagtcagtg
    17701 atagacttga ttttaccttc aacacggtcg cccttgttgt gggtttccgc gaactgctgc
    17761 cacgggttag ctttgcactg tttcagccct agggagatac gacgacgttc ttcgtcgata
    17821 tccagaacca taacttccac tacatcgcca acgttaacaa ctttggacgg gtggatgttt
    17881 ttgttggtcc aatccatttc ggaaacgtgt accagacctt caacgccttc ttcgatttca
    17941 acgaagcagc cgtagtcagt caggttggtc acgcgaccag tcagtttggt accttccgga
    18001 taacgtttag cgatagctac ccacggatct tcgcccagct gtttcaagcc cagggataca
    18061 cgggtacgtt cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc cacgttgacg
    18121 atttcgctcg gatgcttaac gcgtttccag gccatgtcag tgatgtgcag caggccgtca
    18181 acgccgccca gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat acctttaact
    18241 tccatgcctt cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg
    18301 ataacggcac gacgagaaac aacaacgttg ttgcgcttct gatccagctt gattacttta
    18361 aattcaagct ctttgccttc caggtgcaga gtgtcacgca ccggacgaac gtctaccaga
    18421 gaaccaggca ggaacgcacg aataccgttc agctcaacag tgaagccgcc cttaactttg
    18481 ccgttgataa caccggtaac agtttcagca tcttcgtaag ctttttccag cgtgatccag
    18541 gcttcgtgac gtttagcttt ctcacgggac agcagagttt caccgaagcc gtcttctact
    18601 gcgtccagag caacgtcaac ttcgtcacct acctggattt ccagctcgcc ctgggcgttt
    18661 ttgaactgct cagccgggat ggcggactca gatttcagac cagcgtcaac cagtactacg
    18721 tctttgtcga tagcaacaac aacgccacga acgatagaac ccgggcgggt ttcgatttct
    18781 tttaaggact cttcaaagag ttgagcaaaa gattcagtca tgtttaatct tcaggtttat
    18841 atttaacgtc cacctggctc catgccggat ggggttgttt aacatacccg ctgtcattcc
    18901 attgcaacgg gggtactgca aattcggtcg cttatgcgag agccaatttc tggcgcgcgt
    18961 attgtagcgc tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc aacactaaag
    19021 catcggctgc cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt
    19081 tgatctcggc caaaaggcgc tcgaagttaa cactaaagcc cttctcctgc aactgtagca
    19141 tgcggcgatg cgcacgttct tccgaggagg cgtcaaggaa aattttcact ggtgcatcag
    19201 ggaataccac cgttcccatg tcgcggccat cggcaatcag acctggtaat tcgcgaaacg
    19261 cgcgttggcg acgcaataat gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg
    19321 cattcgccac ttcctgagta cgaatttcgc cgctgacatc ttccccttcg aggatcactt
    19381 ccagattgcc attggtcgac acaaaacgta catccagatg ggatgccagc ggtaccagcg
    19441 catcttccga cgcaacatca acatggtgat gtaatgccgc caatgccagt acgcgataaa
    19501 ttgcacccga gtccagcaga tgccattgca acgcttccgc catagcctta cacaaggtgc
    19561 ctttccctgc accgcttggg ccatcaatgg taataaccgg ggcaattgcc gtcatcttta
    19621 tctccttaaa acaggcgtac cgttaacata accgcgcgca ttatacgcgc cgacgccaca
    19681 aatcgttaac gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg atttattgcg
    19741 caattatagc gggctggcag aagaccagcc cgaaagatga caatgattta cttaccagag
    19801 gcgatacgat cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg
    19861 gactgggtgc gaccgccatc caggctagcc agtttttcaa agctgccaac cagcccctgg
    19921 gtgctgatgc cacgtttctt caacagatca aaagagaaat catctgcttc ggactcttta
    19981 tcacgggagt atttagcatt aatagcgcct tctgcgatat cacccaattg tgagcgggaa
    20041 agctgggaag ccacgccgct ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa
    20101 gaagctttca tttcagccag cgagtgaccc aacgcgacgt ggcccagttc atggcccaga
    20161 acaccttcaa tttcgttgtc attcatcatg tccatcaggc cactgtagac acgaacacag
    20221 ccgttcgcca tcgcccatgc gttgacgtcg ctggtcatat aaaccttata gttgaccggc
    20281 gtgccattaa tattgttacc caatgctttg gcgatttttg ccagacgttt accgtacttg
    20341 ctggatttac ttgcgacttg attgccgctg tccatttcgg cacagccctg attggcaatc
    20401 gcttttgcat cggcatcgct taatgttgct gctttatagg catttaaacc ggagctgata
    20461 gccatattgg tatcaatacc gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc
    20521 gcaatcgcca gcagtaattt agtattcttc atgtgaatca tccgttgatt aatcatatat
    20581 aaatattatg tatcaacaac ttacattgac aagcgacgct accattaatc aaaatgactc
    20641 acaaggtccg aaaaaaaacg ccgacaatgt gccgtaatgc cagtcagtta a
//
LOCUS       DADMGC010000071        20548 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.071, whole genome shotgun sequence.
ACCESSION   DADMGC010000071 DADMGC010000000
VERSION     DADMGC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.071"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="JNZ73_002466"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="JNZ73_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAY8732713.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="JNZ73_002467"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="JNZ73_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HAY8732714.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="JNZ73_002468"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="JNZ73_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HAY8732715.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JNZ73_002469"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JNZ73_002469"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HAY8732716.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFCKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JNZ73_002470"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JNZ73_002470"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HAY8732717.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4448..4813
                     /locus_tag="JNZ73_002471"
     CDS             4448..4813
                     /locus_tag="JNZ73_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HAY8732718.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISD
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4963..5481
                     /locus_tag="JNZ73_002472"
     CDS             4963..5481
                     /locus_tag="JNZ73_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4154 domain-containing protein"
                     /protein_id="HAY8732719.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5471..6696
                     /gene="dgcN"
                     /locus_tag="JNZ73_002473"
                     /pseudo
     CDS             5471..6696
                     /gene="dgcN"
                     /locus_tag="JNZ73_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6712..7194
                     /locus_tag="JNZ73_002474"
     CDS             6712..7194
                     /locus_tag="JNZ73_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAY8732720.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQLQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7271..7618)
                     /gene="rplS"
                     /locus_tag="JNZ73_002475"
     CDS             complement(7271..7618)
                     /gene="rplS"
                     /locus_tag="JNZ73_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAY8732721.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7660..8427)
                     /gene="trmD"
                     /locus_tag="JNZ73_002476"
     CDS             complement(7660..8427)
                     /gene="trmD"
                     /locus_tag="JNZ73_002476"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAY8732722.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8458..9006)
                     /gene="rimM"
                     /locus_tag="JNZ73_002477"
     CDS             complement(8458..9006)
                     /gene="rimM"
                     /locus_tag="JNZ73_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAY8732723.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9025..9273)
                     /gene="rpsP"
                     /locus_tag="JNZ73_002478"
     CDS             complement(9025..9273)
                     /gene="rpsP"
                     /locus_tag="JNZ73_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAY8732724.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9410..10771)
                     /gene="ffh"
                     /locus_tag="JNZ73_002479"
     CDS             complement(9410..10771)
                     /gene="ffh"
                     /locus_tag="JNZ73_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAY8732725.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10863..11729
                     /locus_tag="JNZ73_002480"
     CDS             10863..11729
                     /locus_tag="JNZ73_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HAY8732726.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLVIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLVHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIVQQLIS"
     gene            11750..13036
                     /locus_tag="JNZ73_002481"
     CDS             11750..13036
                     /locus_tag="JNZ73_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY8732727.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13091..13684)
                     /gene="grpE"
                     /locus_tag="JNZ73_002482"
     CDS             complement(13091..13684)
                     /gene="grpE"
                     /locus_tag="JNZ73_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAY8732728.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13807..14685
                     /gene="nadK"
                     /locus_tag="JNZ73_002483"
     CDS             13807..14685
                     /gene="nadK"
                     /locus_tag="JNZ73_002483"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HAY8732729.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14771..16432
                     /gene="recN"
                     /locus_tag="JNZ73_002484"
     CDS             14771..16432
                     /gene="recN"
                     /locus_tag="JNZ73_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAY8732730.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16581..16922
                     /gene="bamE"
                     /locus_tag="JNZ73_002485"
     CDS             16581..16922
                     /gene="bamE"
                     /locus_tag="JNZ73_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HAY8732731.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16984..17274)
                     /locus_tag="JNZ73_002486"
     CDS             complement(16984..17274)
                     /locus_tag="JNZ73_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HAY8732732.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17264..17740)
                     /gene="ratA"
                     /locus_tag="JNZ73_002487"
     CDS             complement(17264..17740)
                     /gene="ratA"
                     /locus_tag="JNZ73_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HAY8732733.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGG
                     WKFTPLSQEACRIEFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17872..18354
                     /gene="smpB"
                     /locus_tag="JNZ73_002488"
     CDS             17872..18354
                     /gene="smpB"
                     /locus_tag="JNZ73_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAY8732734.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18569..18931
                     /gene="ssrA"
                     /locus_tag="JNZ73_002489"
     tmRNA           18569..18931
                     /gene="ssrA"
                     /locus_tag="JNZ73_002489"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19136..20377
                     /locus_tag="JNZ73_002490"
     CDS             19136..20377
                     /locus_tag="JNZ73_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY8732735.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDDDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIEPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRQCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5305 a         5368 c         4822 g         5053 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac catcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcgct ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcag aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccaa gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc agtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcattaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat atataaattt acaccccttt gaaaacaatc cgctatgctt tgaaaaagga
     4441 gaaagaaatg atgaaaaagt ttatcgcccc cttgttggct ttactggtta gcggatgtca
     4501 gattgatcct tatactcacg cgccaacctt gaccagcacc gactggtatg atgtcggtat
     4561 ggaagatgcg atatcggaca gcgccataaa agatgacgat gcatttagcg attcacaggc
     4621 ggatcgcggt ctatacctta aaggatatgc cgaagggcaa aagaaaactt gccagaccga
     4681 ttttacttat gcccggggac tttccggtaa aagctttcct gccagctgca acaatgttga
     4741 aaatgccagc caactgcatg aagtatggca aaaaggggct gatgaaaatg ccagcacaat
     4801 acgattaaat taattaaaac cccacaaata aattaaacat aagattttac tcattgtgtt
     4861 ttatttctca cattgatgac ggtcgcatga aaaaatgata ttttaatgag catcctgaaa
     4921 aaatatcgat gcatttcgag cgaagatggt ggggatccct gaatgcgttt ttctcaccga
     4981 ctgttcttac ttctgattct cctgctgacg ggagcaccaa tactcgctca agagccttct
     5041 gacgttgcga aaaacgtgcg tatgatggtt tccggtattg tcagttacac ccgctggcct
     5101 gcgctatcag ggccaccaaa gttatgtatc ttttcatctt cacgttttag taccgcccta
     5161 caagagaatg ccgcgacatc tttgccttat ctgcctgtca tcattcacac tcaacaagag
     5221 gcgatgattt caggctgcaa tggtttttat tttggcaacg agtcaccgac attccagatg
     5281 gaattaaccg aacaatatcc gtcaaaggcg ttattattaa ttgccgaaca aaataccgag
     5341 tgcattattg gtagcgcctt ttgtctgatc atccacaata atgacgtcag atttgccgta
     5401 aacctggatg ccttatcgcg tagcggtgta aaggtcaacc cggatgtctt aatgctcgca
     5461 cggaagaaaa atgatggata acgataattc tcttaataag cgccccacgt ttaaaagagc
     5521 attacgcaac atcagtatga ccagcatatt tatcactatg atgttgatct ggttgctgct
     5581 ttccgtgacc tcggtgctga ccctgaaaca gtacgcgcaa aaaaacctgg cactgacagc
     5641 agcaacaatg acttacagtc tggaagcagc tgtcgttttt gccgatggcc ctgcagcaac
     5701 tgaaacactg gcagcgctgg gccagcaagg gcattttcaa ctgcagaagt acgtgataag
     5761 cagcaaaata ttctggcatc ctggcattac ccccgtaagg atccaggcga tactttcagc
     5821 aatttcataa gccactggct cttccctgcc cccatcattc agccgattcg tcacaatggt
     5881 gaaaccattg gcgaagtacg cttaaccgct cgcgacagtt caattagcca ttttatctgg
     5941 ttttcactcg ccgtactgac cggttgtatt ctgctggcat caggcatcgc aattaccctc
     6001 acccgccatt tgcacaatgg cctggtggaa gcactgaaaa atatcaccga tgtcgtacat
     6061 gatgtgcgtt ccaaccgcaa tttttcccga cgagtttcgg aagaacgtat cgctgagttt
     6121 caccgcttcg ctctcgactt caacagtctg ctggatgaaa tggaagagtg gcagcttcgt
     6181 ttacaggcca aaaatgcgca gcttctgcgt accgcgctac atgacccatt aaccgggctg
     6241 gctaaccgcg cagcgtttcg tagcggcatc aatacgttga tgaacaattc cgatgcccga
     6301 aaaacgtcgg cgttactatt tcttgatggc gataatttca aatatatcaa tgatacctgg
     6361 ggtcatgcga cgggcgatag ggtcttgatt gaaatcgcaa aacggttagc tgaatttggc
     6421 gggctgcgac ataaagcata ccgcctgggc ggcgatgaat tcgctatggt gctctatgat
     6481 gtacagtcgg aatctgaagt gcagcagata tgctcagcac tgacacaaat ctttaatctc
     6541 ccgtttgatc ttcataatgg ccatcagacc accatgacat taagcattgg ttacgcgatg
     6601 accattgagc acgcctctgc ggaaaaatta caagagcttg ccgatcacaa tatgtatcag
     6661 gccaaacacc agcgtgccga aaagctggtg agataacaag gatatatcga tatgataaag
     6721 cacctggtag cacccctgat tttcacctca ctaatactga ctggctgcca gtcccctcag
     6781 ggaaagttta ctcctgagca agtcgccgct atgcaatctt atggatttac tgaatccgcc
     6841 ggcgactggt cgctgggctt atcagatgcc attctgttcg caaaaaatga ctacaaattg
     6901 ctcccggaaa gccagcaaca gctccaaacc atggcagcca aattggcctc gacagggcta
     6961 acacatgccc gtatggatgg acacaccgat aactatggtg aagacagtta caacgaaggc
     7021 ttatcattga aacgggcgaa tgtcgtggcc gatgcatggg ctattggtgg acaaattcca
     7081 cgcagcaatc tcaccacaca gggtttagga aaaaaatatc ccatagccag taacaagacc
     7141 gcccagggcc gcgccgagaa ccgccgcgtc gcagtggtga ttacaacccc ttaaaacacg
     7201 agcaaacaga caaaaaaagg gccagccaat tgcccagccc ttcttaacag gatgtcgctt
     7261 aagcgaaatc ttagttaaga cgctctttga tacgagcagc cttaccagta cgctcacgca
     7321 ggtagtacag tttagcttta cgaacagcac cacgacgttt gacagaaatg ctgtcaacta
     7381 ccggagagtg agtctggaag acacgctcaa cgccttcgcc gttggaaatt ttacgaacag
     7441 tgaatgcaga gtgcagaccg cggttacgaa tagcgataac cacgccctcg aatgcctgca
     7501 gacgtttttt ggaaccttca acaacccata ctttcacttc cacggtatca cccggacgga
     7561 aggaaggtac gtcctgcttc atctgctctt gttcaagttg cttaataatg ttgctcataa
     7621 tttaatctct tatcctgggt aaactgatct ctcgggggct tacgccatcc catcatgttt
     7681 atgttgctgt tgtgcgtgtt ccgttttgaa ctccgccagc aaccttgctt gctcttcagt
     7741 cagagccagg ttttccagaa gttcaggtct tctaagccag gtacggccca gcgactgttt
     7801 caaacgccag cgacgtatct cggcatggtt gcccgacagt aacactggcg gaacttccat
     7861 cccttctaac acctcaggcc gcgtatagtg cgggcaatcc agcaatcctt cagcaaagga
     7921 atcttccgtt gccgaggctt catgtcccag tactcccgga ataaaccggg aaacggagtc
     7981 aatcagcgtc attgctggta actcaccacc actgagaacg taatcgccga ttgaccattc
     8041 ttcgtcaatt tcggtttgga tcacgcgctc atctatacct tcgtagcgac cgcacaccag
     8101 aatcaatttt tgattcgttg ccagttcgct gacgcccgct tgatcaagct tgcgtccctg
     8161 tggtgacaga taaatcacct ttgcgccttc acccgccgcg gcttttgctg catgaatggc
     8221 gtcccgcaag ggttgcacca tcattaacat ccccggtccg ccgccgtaag gacgatcgtc
     8281 cacggtacgg tgccggtcat gcgtgaagtc gcgaggactc caactctgga tgctcagcag
     8341 gccattttta actgcccggc cagttacccc gtaatcggta attgcgcgga acatttcagg
     8401 aaacaggcta attatgccaa tccacatagc gccgtctttt accgtttatc cggtggttta
     8461 aaaaccagga tcccaatcta cttcgattga acgagtagtg agatcgactt tcttgataac
     8521 ctgcccatcg aggaacggta cgagacgttc cttgatacca aacgcatctt tcaggtttgc
     8581 cttaatgacg agaacgtcat tagatccggt ttccatcata tcgacgactt taccgagatc
     8641 gtagccttca gtggttacta cctggcagcc catcaggtct ttccagtagt agtccccctc
     8701 ttcaagctga ggcagctgcg atgaatccac gacaatttca caattcgtca gcaggttcgc
     8761 cgcatcacga tcgtcaacgc ctttcagctt gatgatcatg tcctgattgt ggtgcttcca
     8821 gctttccagc tggacttgct gccactgacc cgccttctgg ataaaccagg gctgatagtc
     8881 aaaaatgctt tcggcgtctt cggtggaaga aaacactctg agccacccac gaataccgta
     8941 agacgaaccc atttttccca aaacgatggg atcaacaggt gcttgcgcgg tgagttgttt
     9001 gctcatcatg accaccgtga cagattaagc tgctttgttt acttctttga tcagcgcagc
     9061 aacgcgatca gaaatagttg cgccctggcc aacccagtga gcgatgcgat ccagatccag
     9121 gcgagtgcct tcttcttttt cgctagcgat tgggttgaag aaaccaacgc gctcgatgaa
     9181 gcgaccgttg cgtgcattac ggctgtcagc gacaacaacc tggtagaacg gacgcttttt
     9241 agcgccgtga cgtgctaaac gaatagttac cataacatcc tcttgtgtga ataaaacaac
     9301 cggaccccat cgaggaacgg agtccggtgt catattaaaa gcccgaaaat tttactgatt
     9361 tctggagaaa atgcaatcag cagttaataa ctctgtcgca taaggtcatt tagcgaccag
     9421 gaaatcccgg cggcatcatt cccttcatgc ttctcatcat cttcgccatt ccgcctttct
     9481 tcattttctt catcatgcgc tgcatgtcgt cgaactgctt cagaaggcgg ttaacgtcct
     9541 gtacctgcat cccgcaaccg gcggcgatac gacgcttacg cgaacctttg atgatttctg
     9601 gcttagcgcg ctctttcatc gtcattgagt tgatgatggc ttccatacgc accagcactt
     9661 tatcgtccat ctgcgacttg acgttatccg ggatctgccc catgcccggc agtttgccca
     9721 tcaagctggc catgccgccc atatttttca tctggcgcag ttgctcaaga aagtcgttga
     9781 gatcgaagcc gtcgcctttt tttagcttgc tggctaattt ctctgcctgc gcgcggtcaa
     9841 ctttgctttc gatatcttcg atcagcgaca gtacgtcgcc catgccgaga atacgcgacg
     9901 cgatgcggtc cggatggaac ggctccagcg cctcagtttt ctcgccaaca ccgaggaact
     9961 tgatcggttt gccagtgatg tgacgaatag agagcgccgc accgccgcgg gcatcgccgt
    10021 ccactttggt caacactacg ccggtaagcg gtaacgcttc attgaatgct tttgccgtat
    10081 tggccgcatc ctgaccggtc atggcgtcaa ccacaaacag ggtttcaacc gggttaatcg
    10141 acgcatggac ttgtttgatc tcgtccatca tcgcttcgtc aacgtgcaga cgaccagcgg
    10201 tatccaccag cagcacgtcg tagaatttca gtttggcttc tttcagcgcc gcgttaacaa
    10261 tatcaaccgg cttctgacca acatcagaag ggaagaaatc aacgcccacc tgctctgcca
    10321 gcgtctcaag ctgtttgatt gccgccgggc gatagacgtc agcagaaacc accagcactt
    10381 tcttcttgtg cttctcgcgc aggaaattac cgagtttacc aacgctggtt gttttaccgg
    10441 caccttgcag gcctgccatc agcacgaccg caggcggttg cgcagccaga ttcagggtct
    10501 ggttctcttc gcccatcgcc gcaaccagtt cgttacggac gattttgacg aactcctgcc
    10561 ccggcgtcag gctcttatta acttcatgac caaccgcttt ctcttttacg cgattgataa
    10621 actcacgcac taccggcaga gcgacgtccg cctccagcag cgccatgcgc acttcgcgca
    10681 gcgtatcttt tacgttgtct tcagtgaggc gtccacggcc actgatattg cgcagcgtgc
    10741 gcgacaaacg atcggttaaa ttatcaaaca ttgtctctcg cctggggtgg aaacggttgg
    10801 ccgcaatcgc gacacatcat cagtattttg ccgcagtata acatgaaggc gtctttgttg
    10861 ttatgcaacg gttggagcag cgttcacctg acgctatact gcttctcttt cttattgctc
    10921 aaactgtcga cattactatg cccgtttttg ctctgctcgc gcttgtcgcc tactccgtca
    10981 gtcttgcgct gattgttccc ggtctgctgc aaaaaaacgg cggctggcgg cgcatggcta
    11041 ttatttctgc ggtcattgcg ctggtctgcc acgcaatcgc tctggaagcc cgcatcctgc
    11101 ccgacggtga tagcggacaa aacctcagcc tgctgaacgt tggttcattg gtcagtttgg
    11161 tgatctgtac ggtaatgacc attgtggctt ctcgcaatcg tggctggttg ctgctaccca
    11221 ttgtctatgc ctttgcgctt atcaacctgg cgctggcgac cttcatgccc aatgaataca
    11281 tcacccatct ggaagcaacg cctgggatgc tggtgcacat tggcttatcg ctcttttcct
    11341 atgccacgct aattatcgcc gccctgtacg cgctgcaact ggcgtggatt gattaccaac
    11401 tgaagaacaa gaagctggca tttaaccagg aaatgccgcc attgatgagt atcgagcgta
    11461 aaatgttcca catcacgcag attggcgtgg tgctgctaac gctcacgctt tgcactggcc
    11521 tgttctacat gcacaaccta tttagcatgg aaaatatcga caaggctgtg ctctctatcg
    11581 tggcgtggtt tgtctatatt gtgctgctgt ggggacatta ccatgaagga tggcgtggac
    11641 gccgcgtcgt ctggtttaac gttgcgggcg cggtaattct gacactggcc tacttcggca
    11701 gccgaattgt ccagcagtta atcagctaaa cccagaaaag gagtttcccc tggaacacat
    11761 ttctactact acgttgatca ttattctgat catcatggtg gtcatttcag cctatttttc
    11821 cgggtccgaa accggaatga tgaccctcaa ccgctatcgt cttcgacata tggcgaaaca
    11881 gggtaatcgc tcggccaaac gcgtcgaaaa attgctgcgt aagccagacc gcctgataag
    11941 cctggtgtta atcggcaata acctggtcaa tattcttgcc tccgcgctcg gcactattgt
    12001 cgggatgcgt ttgtacggcg atgcgggcgt ggcaattgcg actggtgtgc tgacttttgt
    12061 cgtactggtt tttgctgagg tattgccgaa aaccattgcc gcgctgtacc cggaaaaagt
    12121 cgcttatccg agtagttttc tgctggctcc gctgcaaatt ttgatgatgc cgctggtctg
    12181 gttgctgaat gctatcaccc gtatgctgat gcgcatgatg ggtatcaaaa ccgatatcgt
    12241 ggttagcggc tctttgagca aagaagagtt gcgcactatc gtgcatgaat cgcgctcaca
    12301 aatttcccgt cgcaatcagg atatgctgct gtcggtgctc gatctggaaa aaatgaccgt
    12361 tgatgacatc atggtgccgc gcagtgaaat tatcggtatt gatatcaacg atgactggaa
    12421 atctattctg cgccaactct cccactcacc tcacgggcgc atcgtgcttt accgtgattc
    12481 gctggacgac gccatcagta tgctgcgagt acgtgaagcc tggcggttga tgtcggagaa
    12541 aaaagagttc accaaagaaa ccatgctgcg cgccgcggac gagatctatt ttgtgcctga
    12601 aggtacgccg ctcagcacgc agttggtaaa gtttcagcgc aacaaaaaga aagtcggcct
    12661 ggtcgtcaac gagtatggag acattcaggg gctggtgacg gttgaagata ttctggaaga
    12721 gattgtcggc gatttcacta cgtcgatgtc gccaacactt gccgaagagg tcacgccgca
    12781 aaacgacggt tcggtgatta tcgatggcac cgccaacgtg cgggaaatca acaaagcctt
    12841 taactggcat ctaccggaag atgatgcccg cacggttaat ggcgtcattc ttgaggcact
    12901 ggaagagatc cctgtcgcag gcacccgcgt gcgtattggc gagtacgata tcgatattct
    12961 cgacgtacag gacaatatga ttaagcaggt aaaagttttt cctgtgaaac cgctgcgcga
    13021 gagtgtggcg gagtaacgaa aacggccctg cattcgcatg cagggccgtg aattattacg
    13081 aaaacagaaa ttacgctttt gctttcgcta cagttaccat cgccgcacga atcgtacgac
    13141 cattcagcgt ataacccttc tgcataatgc ccagtacgtt acctggcgca acgtcatcag
    13201 attccaccat tgcgatagcc tgatgcacat tcgggtccag cggcacgtta gtttcggcga
    13261 tcacttcaac accaaactta cgcacaacat ccagcatcga cttcagcgtc agctcaatgc
    13321 cttcaaccat cgcagacata tccgggttag ctttatcagc cacttccagc gcacgatcca
    13381 ggctatcaat caccggcagc aattcgttga tgaatttctc cagcgcgaat ttgtgggctt
    13441 tttcaatatc cagttcagta cgacgacgca ggttttccat ttcggctttt acgcgcaaaa
    13501 taccgtcacg ttcacgggtc tgggcttcag ccagctgagc ttcgagattc gcaatttttt
    13561 catcgcgcgg atccacctgc tcagcagaag cttctggctc aactgcctca atctcttcgt
    13621 gctgatccat gataatttct tccggggctt gcccctcagg cgttttctgt tctttactac
    13681 tcatgaattt ctccgcgttt ttttcgcatt catctcgcta acttcgctta ttatggggat
    13741 cagtttcagg gtttcaaggg aagcactcac attgtcatca atcttcgcaa caaggacctc
    13801 ggaaaaatga ataatcattt caagtgcatt ggcattgtgg gacacccacg gcaccctact
    13861 gcactgacaa cacatgaaat gctctaccgc tggctgtgca caaaaggtta cgaggtcatc
    13921 gttgagcaac aaatcgctca cgaactgcaa ctgaagaatg tgaaaactgg cacgctcgcg
    13981 gagattgggc aactggctga tctcgcggta gtcgttggtg gcgacggtaa tatgctgggc
    14041 gcggcgcgca cgctcgcccg ttacgatatt aaagttattg gaatcaaccg tggcaacctg
    14101 ggtttcctga ctgaccttga ccccgataac gcccagcaac aattagccga tgtgctggaa
    14161 ggtcactaca tcagcgagaa acgttttttg ctggaagcgc aagtctgtca gcaagattgc
    14221 caaaaacgca tcagcaccgc gattaacgaa gtggtacttc atcctggcaa agtggcgcat
    14281 atgatagagt tcgaagtgta tatcgacgag atctttgcgt tttctcagcg atctgatggg
    14341 ctaattattt cgacgccaac aggctccacc gcctattccc tctctgcggg cggtcctatt
    14401 ctgaccccct ctctggatgc gattaccctg gtgcccatgt tcccgcatac gttgtcagca
    14461 cgaccactgg tcataaacag cagcagcacg atccgtctgc gtttttcgca tcgccgtaac
    14521 gacctggaaa tcagttgcga cagccagata gcactgccga ttcaggaagg tgaagatgtc
    14581 ctgattcgtc gctgtgatta ccatctgaat ctgattcatc cgaaagatta cagttatttc
    14641 aatacattaa gcaccaagct cggctggtca aaaaaattat tctaatttta cgccagcctc
    14701 tttactgtat ataaaaccag tttatactgt acacaataac agtaatggtt tttcatacag
    14761 gaaaacgact atgttggcac aactgaccat cagcaacttt gctatcgttc gtgagcttga
    14821 gattgatttt catagcggca tgaccgtaat aactggcgag accggtgcgg gtaaatctat
    14881 tgcaatagat gccctcggtc tttgtctcgg tggtcgcgct gaagccgaca tggtgcgtac
    14941 cggcgctgct cgcgctgacc tgtgcgcccg tttttctctg aaagatacgc cagcggccct
    15001 gcgctggctg gaagaaaacc agcttgaaga cgggcatgaa tgtttgcttc gtcgcgtgat
    15061 cagcagtgat ggtcgctccc gtggtttcat caacggtaca gctgttcctc tgtcacaact
    15121 gcgcgaactg ggtcagttgc tgattcagat ccatggtcag cacgctcatc aattactcac
    15181 caaacctgag caccaaaaat tcctgcttga tggctatgcc aatgaaacct ctctacttca
    15241 ggaaatgacc gcacgttatc agttgtggca tcaaagctgc cgtgacctcg cgcatcatca
    15301 acagttaagt caggaacgcg ccgcccgtgc ggaactgctg caataccaat taaaagaact
    15361 taacgaattt aatccgcagc ccggagagtt tgagcaaatc gacgaagagt acaaacgtct
    15421 ggcgaacagc ggtcaattgc tgaccaccag ccagaatgca ttggcattaa tggccgacgg
    15481 tgaagacgca aacctgcaaa gtcagcttta cacggctaaa caactggtga gcgaattgat
    15541 tggcatggac agcaaactgt ccggcgtact tgatatgctg gaagaagcta ccatccagat
    15601 tgctgaagcc agcgacgaac tgcgccacta ctgcgatcgt ctggatctcg atcccaaccg
    15661 actgtttgaa cttgaacagc gcatctcaaa acagatttcg ctggcacgta aacatcacgt
    15721 cagccctgag gcattaccac agtattacca gtcgctactg gaagaacagc agcaactgga
    15781 cgatcaggcc gactcacaag aaacgctcgc gctggcggta acgaaacatc atcagcaggc
    15841 actggaaacc gcgcgcgcat tacaccaaca acgccagcaa tatgcagaag aacttgcaca
    15901 gctgatcacc gacagtatgc atgcgctctc aatgccgcat gggcagttta cgattgatgt
    15961 caaatatgac gagcatcacc tgggcgctga cggtgccgat cgtattgagt ttcgggtaac
    16021 caccaaccca ggtcagccaa tgcagcctat tgccaaagtc gcatccggtg gtgaattgtc
    16081 ccgcatcgca ctggcaatcc aggtcatcac ggcgcgtaaa atggaaaccc cggcactgat
    16141 ttttgatgaa gtggatgtag ggattagcgg tccaacagcg gcagttgtcg gcaaactgct
    16201 gcgtcaactc ggcgaatcaa ctcaggtgat gtgtgttacc cacctgccac aagtcgcggg
    16261 atgtggtcat caacactatt ttgtcagcaa agaaaccgat ggtgcgatga cagaaacaca
    16321 tatgcaatct ctggataaaa aagcgcggtt acaagagctg gcgcgcctgc ttggtggcag
    16381 tgaagtcaca cgtaacacac tggcgaatgc gaaagaactg cttgcagcgt aaactttttt
    16441 cctgcttcac ggtcagagta aacagcaaaa cgccgtaaga ccggaaagca aaaggtttta
    16501 aagtgatgaa aggtctatta tcatcggcat attacagatg agccacgtac tgctcgggcc
    16561 cgaaaaggaa tcaaatcact atgcgctgta aaacgctgac tgctgcagca gcagtactat
    16621 tgatgttgac cgcaggctgt tccactctgg agcgagtggt ttaccgtcct gacatcaacc
    16681 aggggaacta tctgaccgct aacgacgtat ccaaaatacg tgttggcatg acgcaacaac
    16741 aagttgcgta cgcattgggt acaccgctga tgtccgatcc atttggtacg aatacctggt
    16801 tctatgtctt ccgccagcaa ccaggtcatg aaggtgtaac gcagcaaacg ctgacgctga
    16861 cctttaacag tagcggtgtg ttgaccaata ttgataacaa acctgcgctg agtggtaact
    16921 aataataaag ttgctctcaa agacgttaaa aaaggtgctc aatgagcacc ttttttctgt
    16981 ctgttattta ttcgctgatt tttctgctcg ttgcctgcga agctctttcg gatcggcaat
    17041 gagagggcga taaatctcca cccgatcgcc atcatgcaca ctatcgctta gttttgccgg
    17101 acggctgtaa atgccgactt tgtttttagt taaatcgata tcggtacgca attccagcaa
    17161 gccactggcg cgaatagctt cttcaaccgt cgcgccctcc tgcagcgtca ctcgctgcag
    17221 gtactgcttc tcaggtagcg cataagccac ctcaacggca attttaccta gcactgtaaa
    17281 cctctttcgc acgaaccgta aaagcctgga ccatattagc cgccagctct ttaaacacgc
    17341 gaccaaaggc gagttcaatc aacttattgg taaactcaaa gtcgagatga aactcgatac
    17401 gacacgcctc ctggctcagc ggcgtaaact tccatccacc aatcaatttc ttgaacggcc
    17461 catccaccag attcataaga atactttggt tactggtcaa ctggttgcgg gtagtaaacg
    17521 ttttgctgat cccagcctta gagacatcta ccgccgcagt catctgccca ggagtggact
    17581 ccagaatccg acttccggta caacctggca aaaactgagg ataagactga acgtcattca
    17641 ctaactgata catttgctcc gcgctgtagg gtaccagtgc ggtccggcta atctgaggca
    17701 taacaatttc catcaacaaa aatccaacaa ataatatcat ttatcctgct gtaaaaaaaa
    17761 cgctatcccg gcgctgggta acatcgggtt catgctaaga tagagccttg tcccccgcag
    17821 gattgatatg gggtgttttc gatttcagat taccgatgat tcacgacgct tatgacgaag
    17881 aaaaaagcac ataaacctgg ttcagcgacc atcgcgctta acaagcgcgc ccgtcacgaa
    17941 tactttatcg aagaagagtt cgaagcggga cttgccctgc aaggctggga agttaaatcc
    18001 ctgcgcgcag gaaaagccaa tatcagcgac agctacgtcc ttctgcgtga cggagaggca
    18061 tttctgtttg gcgctaacat cacgccaatg gccgtggcct ccacgcatgt ggtgtgcgat
    18121 cctacccgta cccgcaagtt acttctcaac cagcgcgaac tggactcatt gtacggtcgc
    18181 gtcaatcgag aaggctatac cgtagtggcg ctctccctgt actggaaaaa tgcctggtgc
    18241 aaagtgaaaa tcggcgtcgc caaaggtaaa aaacagcacg ataaacgttc agatatcaaa
    18301 gaacgcgaat ggcaggtgga taaagcacgc atcatgaaaa acgcccaccg ttaaacctgc
    18361 actccaatta ttgaccagtt cctcaccgcg cctccctctc cggcggcgcg aatgaacatc
    18421 ttattggcta tcacatccga cacaaatgtt gccatcccat tgcttaatcg aataaaaatc
    18481 aggctacatg ggtgctaaat ctttaacgat aacgccattg aggctggtca tggcgctcat
    18541 aaatctggta tacttacctt tacacattgg ggctgattct ggattcgacg ggatttgcga
    18601 aacccaaggt gcatgccgag gggcggttgg cctcgtaaaa agccgcaaaa aatagtcgca
    18661 aacgacgaaa actacgcttt agcagcttaa taacctgctt agagccctct ctccctagcc
    18721 tccgctctta ggacggggat caagagaggt caaacccaaa agagatcgcg tggaagccct
    18781 gcctggggtt gaagcgttaa aacttaatca ggctagtttg ttagtggcgt gtccgtccgc
    18841 agctggcaag cgaatgtaaa gactgactaa gcatgtagta ccgaggatgt aggaatttcg
    18901 gacgcgggtt caactcccgc cagctccacc aaaattctcc atcggtgatt accagagtca
    18961 tccgatgaag tcctaagagc ccgcacggcg caagccctgc gggctttttt gtgccccgaa
    19021 tttgtcccgc gaagtctgat gccaactaat taaatccgat ccttttaggc accttgttag
    19081 gcacttcata aagctatatt gtctttgagg tgcctaaaac tatggaaacc cggcaatggc
    19141 aagacaaacc aaacctctat ccgttaagga aatcgaatct gctaaaccca aggaagcgga
    19201 ctacgttctc tatgatgacg atggccttga gctactcatt aaatccagcg ggagtaaaat
    19261 ctggcagttt cgctacattc gccctgtcac caagacacga gcaaagaaaa gcataggccc
    19321 ttacccgtca gttacccttg ccgatgccag aaactaccgt gcagagtccc gctcactcct
    19381 agctaaacag attgagccgc aggaacatca gcaagaacaa ttgcgtagtt cgctggaagc
    19441 caaaaccaat actttccagc tcgtagctga acgttggtgg aatgtgaaga aagccagtgt
    19501 gacagaggac tatgcggaag atatctggcg ctctcttgaa agagatgtct ttcctgcgat
    19561 tggcgacgtt agcgttacag atattaaagc tcatacactg gttcaggccg tacaaccggt
    19621 tcaggccaga ggagcactgg aaacggttcg tcgccagtgt caacgtatta atgaggtcat
    19681 gatctatgcc cagaacacag ggctgattga tgctgttccc agcgttaata tcggtaaagc
    19741 cttcgaaaag cctcagaaaa agaacatgcc cagcattcga ccggatcagc tatctcaact
    19801 gatgcagaca atgcgaacag ccagcattag cctttccaca cgctgcctgt tcatgtggca
    19861 acttcttact attacccgcc ctgccgaagc ggctgaagct cgctgggaag aagtagacat
    19921 agaagcgcaa gagtggaaga ttcccgcagc acgcatgaaa atgaaccgcg accatactgt
    19981 tccattgtca gatgaagcaa tagctgtgct ggagatgatg aaaccgctaa gcggcaaccg
    20041 agaatttatc tttcctagcc gtatcaagcc caaccagcct atgaacagtc agactgttaa
    20101 cgcctcacta aaacgcgcag gttttggtgg tgtgcttgtt tcgcatggtc tgagatcaat
    20161 tgccagtacg gctcttaatg aacaaggctt tccgcctgat gccattgagg cagcattggc
    20221 ccatgtggat aagaatgagg ttcgtcgtgc ttataaccgc agcgattacc tggaacagcg
    20281 tcgcccgatg atgcaatggt gggcaaattt tgtgatggca gcggatcgtg gaagcatgat
    20341 tgaaggtggg ataaaaggaa tgaagctagt tgggtgacta aatagctgcg cggcatagtg
    20401 tcccactttc agccagccct tgcaggttaa gccaccagac agtaaacggc aggtgtcgcg
    20461 ttattaggcg cagctattta gtagtaatat taggattggt gattctgcac tccagaagtt
    20521 cggtaatgct gccaacttac tgatttag
//
LOCUS       DADMGC010000072        20303 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.072, whole genome shotgun sequence.
ACCESSION   DADMGC010000072 DADMGC010000000
VERSION     DADMGC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.072"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            145..297
                     /gene="hokA"
                     /locus_tag="JNZ73_002491"
     CDS             145..297
                     /gene="hokA"
                     /locus_tag="JNZ73_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HAY8732736.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(484..696)
                     /gene="cspA"
                     /locus_tag="JNZ73_002492"
     CDS             complement(484..696)
                     /gene="cspA"
                     /locus_tag="JNZ73_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HAY8732737.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNYGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(977..1267)
                     /locus_tag="JNZ73_002493"
     CDS             complement(977..1267)
                     /locus_tag="JNZ73_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HAY8732738.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIERLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLMK"
     gene            1701..2411
                     /locus_tag="JNZ73_002494"
     CDS             1701..2411
                     /locus_tag="JNZ73_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HAY8732739.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2461..3435)
                     /gene="ghrB"
                     /locus_tag="JNZ73_002495"
     CDS             complement(2461..3435)
                     /gene="ghrB"
                     /locus_tag="JNZ73_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HAY8732740.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3539..4198)
                     /locus_tag="JNZ73_002496"
     CDS             complement(3539..4198)
                     /locus_tag="JNZ73_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HAY8732741.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGVLSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4351..6684
                     /locus_tag="JNZ73_002497"
     CDS             4351..6684
                     /locus_tag="JNZ73_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY8732742.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLAHCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6653..7093)
                     /locus_tag="JNZ73_002498"
     CDS             complement(6653..7093)
                     /locus_tag="JNZ73_002498"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8732743.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7090..7653)
                     /gene="tag"
                     /locus_tag="JNZ73_002499"
     CDS             complement(7090..7653)
                     /gene="tag"
                     /locus_tag="JNZ73_002499"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAY8732744.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKCGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7811..8509
                     /locus_tag="JNZ73_002500"
     CDS             7811..8509
                     /locus_tag="JNZ73_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY8732745.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8738..9946
                     /locus_tag="JNZ73_002501"
     CDS             8738..9946
                     /locus_tag="JNZ73_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8732746.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10270..11961
                     /gene="eptB"
                     /locus_tag="JNZ73_002502"
     CDS             10270..11961
                     /gene="eptB"
                     /locus_tag="JNZ73_002502"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HAY8732747.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASAMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12053..12129
                     /locus_tag="JNZ73_002503"
     tRNA            12053..12129
                     /locus_tag="JNZ73_002503"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12087..12089,aa:Pro,seq:cgg)
     gene            12873..14480
                     /gene="dppA"
                     /locus_tag="JNZ73_002504"
     CDS             12873..14480
                     /gene="dppA"
                     /locus_tag="JNZ73_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HAY8732748.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FESVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14788..15807
                     /gene="dppB"
                     /locus_tag="JNZ73_002505"
     CDS             14788..15807
                     /gene="dppB"
                     /locus_tag="JNZ73_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HAY8732749.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15817..16719
                     /gene="dppC"
                     /locus_tag="JNZ73_002506"
     CDS             15817..16719
                     /gene="dppC"
                     /locus_tag="JNZ73_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HAY8732750.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16730..17713
                     /gene="dppD"
                     /locus_tag="JNZ73_002507"
     CDS             16730..17713
                     /gene="dppD"
                     /locus_tag="JNZ73_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAY8732751.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17710..18714
                     /gene="dppF"
                     /locus_tag="JNZ73_002508"
     CDS             17710..18714
                     /gene="dppF"
                     /locus_tag="JNZ73_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HAY8732752.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18744..20015)
                     /locus_tag="JNZ73_002509"
     CDS             complement(18744..20015)
                     /locus_tag="JNZ73_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY8732753.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4860 a         5028 c         5329 g         5086 t
ORIGIN      
        1 tccaattgaa tatcggaatc agacctatat gcctcgtgtt taactgtcca actttttggg
       61 gtcagtacaa agtgaggcta ccctatgcct gaacaccata aggtgtgcct cttactcgtt
      121 ggaaatcaac acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg
      181 atttgtttca cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat
      241 attaaacagg agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag
      301 tcttcggcgg gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccac
      361 atttattctt cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga
      421 aaaaaaataa aaaaatcccc gcaaaatggc agggatctta gattctgtgc ttttaagcag
      481 agattacagg ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa
      541 ggacactttc tgaccttcgt ccagagattt gtaaccatag ttctggatag cagagaagtg
      601 tacgaacaca tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt
      661 gaaccatttt acgataccag tcattttacc ggacatagtg tattaccttt aataattaag
      721 tgtgcctttc ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact
      781 acgaggggta tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc
      841 tgtacgtcaa accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt
      901 tttcatattt atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa
      961 taaagtggat aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca
     1021 aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta
     1081 ccatagcgac tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt
     1141 ttcgtaaccg ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg
     1201 tttcatcttt aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat
     1261 attccattga aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta
     1321 agaatagtcg ggaaaagcag acacagcggg gttaagtcag ggcatgctct ggctatgatt
     1381 cgattcagcc tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata
     1441 tatctcttat cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta
     1501 aatctctggc tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt
     1561 aaccttgttt cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc
     1621 aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt
     1681 tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc
     1741 ttgcggcgtt attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc
     1801 gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa
     1861 ccctgactgc cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt
     1921 atggttattc tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca
     1981 gcgttaacgc aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa
     2041 tgaatggttc gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg
     2101 ctgcgcactc tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca
     2161 ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt
     2221 tcacgcaaca actggtgatg gttggagact acattgctca gcaaggtact caggtgagct
     2281 ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga
     2341 ttgcgccatt accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg
     2401 caacccaata aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcggc
     2461 ttagtccgcg acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat
     2521 caaattatcc acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat
     2581 atgcggtact gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc
     2641 ttgttcgaag acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag
     2701 tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat
     2761 tttggcgaat tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag
     2821 gcaaacgaaa tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg
     2881 ttcttctgct tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa
     2941 gtgcgcacgt tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt
     3001 atggtgaacg tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt
     3061 tacccgttct gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt
     3121 atcggcgacg gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt
     3181 aagcgcatcg acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt
     3241 cggcattttt tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc
     3301 ttctgcaaaa attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg
     3361 aacggtgaaa tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat
     3421 aacggacggc ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata
     3481 tgatgaagga aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt
     3541 acagcgggct taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt
     3601 tgctggcgat tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc
     3661 cctgggtgat caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca
     3721 ggtcgtgacc ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact
     3781 ctttcagcac cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggcgcgc
     3841 tgctgctgtc gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg
     3901 ttacgctaac accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt
     3961 aataacccac gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac
     4021 cgcgatcttt cttcgaagaa gagagcacac caatacccgc gcccacgaga gagcccagac
     4081 ctgcgccgat agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc
     4141 cagataccgc cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg
     4201 ttaatcctta ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc
     4261 gtgaacatga agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc
     4321 aatacatcta acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt
     4381 ctgactgccg cccactgggg gcccatgctg gttgaaaccg acggcgaaac cgtgtttagc
     4441 tcgcgtggcg cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag
     4501 gttcacagca atacgcgggt acgatttcca atggtgcgca aaggctttct cgcgtcaccg
     4561 gaaaacccgc aaggcattcg tgggcaggat gaatttgttc gcgtgagttg ggatgaggcg
     4621 ctggatctta ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt
     4681 tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta
     4741 caacgctata tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggt
     4801 gcggcacagg cgatcatgcc gtatgtcgta ggtggcagcg aagtttatca acagcaaacc
     4861 agttggccgc tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc taacccactc
     4921 aatacgctga aaattgcgtg gaatgcatcc gatgagcagg ggctttctta cttttccgca
     4981 ctgcgtgaca gtgggaaaaa gctgatctgc attgatccaa tgcgatcgga aaccgtcgat
     5041 ttctttggcg ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgctgatg
     5101 ctggggatcg cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcat
     5161 tgcaccacag gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagcg
     5221 aaaaacgccg aatgggcggc agagatttgt ggtgttggcg cagcgaaaat ccgcgagctg
     5281 gcggctattt tccaccaaaa taccaccatg ctgatggcag gctggggaat gcagcgccaa
     5341 cagtttggtg agcaaaaaca ctggatgatc gtcacgctgg cagcaatgtt ggggcaaatc
     5401 ggcacacccg gcggcggttt tggtctttct taccattttg ccaatggtgg taaccccacg
     5461 cggcgttctg cggtgctctc ttccatgcag ggcagcttgc cgggtggctg cgatgcggtg
     5521 gataaaatcc ctgttgcccg cattgttgaa gcactggaaa accctggtgg cgcatatcaa
     5581 cacaacggta tgaaccgaca tttcccggat attcgtttta tctggtgggc gggcggtgcc
     5641 aactttactc atcatcagga taccaatcgt ctgatccgtg cctggcaaaa accggagctg
     5701 gtggtgatct ctgaatgctt ctggacggcg gcggcgaaac acgcggatat cgttctgcct
     5761 gcgactacct cttttgagcg taatgatctc accatgaccg gtgattacag taatcagcat
     5821 ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt
     5881 tttgccgagt tgagtgaacg ttgggagaag ggcggctatg cgcggtttac ggaaggaaaa
     5941 agtcagctgc aatggctgga aacgttttat aacgttgccc ggcagcgcgg ggcaagccag
     6001 caggttgaat tgccgccatt tgctgagttc tggcaagcca accagttaat tgagatgccg
     6061 gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgctggcg
     6121 catccgttaa aaaccgccag cggcaagatt gaaatcttct cacagcgtat tgccgattac
     6181 ggttacccgg attgccctgg gcatccaatg tggctggagc cggacgaatg gcagggcaat
     6241 gccgaaccag aacagttgca ggtactttct gcccatccgg cgcaccgtct gcacagccag
     6301 ctgaattaca gttctctgcg cgaattgtac gcggtagcaa accgtgagcc tgtcaccatt
     6361 catcctgacg atgctcaggc gcgcggcata accgaagggg atatggtgcg ggtgtggaac
     6421 tcccgtgggc agatccttgc tggagcggtc attagcgagg gaattaaacc tggcgtgatt
     6481 tgcattcacg aaggggcatg gccggatctg gatttaaccg ctgacggtat ttgtaaaaac
     6541 ggcgcagtga acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg
     6601 ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg
     6661 tttgaaccac cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc
     6721 aatcgacaat gtgaaaaccc tgcgcacggt aaaaatctat cgccggttga tttttttgat
     6781 aaacctccag catcagatgg ggatagcgct gctgcacata ctgcatcagc gccttaccaa
     6841 taccgcgtct gacggccttc ggtgcgacaa acattgctgc cagaaatcgg ccttccataa
     6901 tgctgacaaa accgagaagc ttaccgtcct cttcccagac ccagttttgc gcgttggcaa
     6961 gataggcatc ccgcaccagc ggaatgcagt cacgccagta attcgctttt ataaagggat
     7021 gcccccaggt tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg
     7081 cttcccgaat catggtttat ttcccggata gcaacagcag ccaaccacat gatcattcac
     7141 cagcccacat gcctgcataa aggagtaaca gattgtggtg ccgacaaact taaaaccaca
     7201 ttttttcagt gccttagata gggcgtcgga ggcggacgta gatgtgggaa tttcgctcaa
     7261 cgttgtggct tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcgggaaa
     7321 cggttcgccg ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat
     7381 tttccctcga tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg
     7441 cattgctgcg accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt
     7501 tttgaggacg gtgatccacg ataacccagc ctgctgccct tcaaggcaga tcatttcgaa
     7561 cagtttttta ctgtcagttt caggcacgcc ccactcatta tcatggtagg caatataaag
     7621 cgggccctga ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt
     7681 aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct
     7741 actatgcccg atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg
     7801 agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag
     7861 cgtggtaatc agtgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt
     7921 tgatacgcaa cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga
     7981 ttacaacgat attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt
     8041 caatctggcg gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt
     8101 tcctttatat gaacaggtta caaccggccc ggtcgcggca ttacattacg atggcacaac
     8161 cacctcgatg tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca
     8221 tgccagcgtg agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg
     8281 ggcgcaaatc agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct
     8341 gagccggatg acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga
     8401 tatgttgctc aatcaaaata ttgccgccta tgctgcgcta actcaggcag aaaataccac
     8461 taataatagc gactatctgt atacgatggg ggttagcgcc agattttaac gtaacagtca
     8521 caattgaaac cattgaataa caacagttgt ggcgatagtg ggtgctaact taccaaataa
     8581 taaatttggt gaataattgt cgcgtcattc attcctgaac taaggcattt cattccgttc
     8641 tgatggcatt tcatgccgtt tttccccaag cataaaatgc acttcgttat ggttgtcggc
     8701 agagattttt cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg
     8761 tacccgctgg ctgacactca tcggtactat cattacccag tttgcgctgg ggtcggttta
     8821 tacctggagc ctgtttaatg gcgcgctttc tgccaagctg gatgcgccgg taagccaggt
     8881 cgctttctct ttcggcttgt taagtctggg gctggcaatt tcgtcttctg ttgcgggcaa
     8941 attgcaggaa cgttttggcg ttaaacgcgt caccgtggct tccggcattt tgctgggatt
     9001 aggcttcttc ctgaccgcgc attccaacaa cctgatgatg ctgtggttaa gcgccggtgt
     9061 gctggtcggt ctggcggatg gcgcgggtta cctgctgacg ctctctaact gcgtgaagtg
     9121 gttcccggag cgtaaaggtc tgatctccgc gttcgctatc ggttcttatg gtctgggtag
     9181 cctgggtttc aaatttatcg acacgcagct gctcgaaacg gtgggtctgg aaaaaacctt
     9241 tgtgatttgg ggagcgattg cgctggtgat gattgttttc ggcgcaacgt taatgaaaga
     9301 cgcaccaaaa caggaagtga aaaccagcaa tggtgtggtg gagaaagatt acacgctggc
     9361 agagtcgatg cgtaaaccgc agtactggat gttagcggta atgttcctga ctgcctgcat
     9421 gagcggcctg tacgtgattg gggtagcgaa agatatcgcc caaagtctgg cacaccttga
     9481 tgtggtttcc gcagccaatg cagtaacggt tatttccatc gctaaccttt caggtcgtct
     9541 ggtgctgggc attctgtctg acaaaattgc ccgtatccgt gttatcacca ttggtcaggt
     9601 gatttcgctg gtgggtatgg cggccctgct gtttgcacca ttaaatgcag tgacgttctt
     9661 tgcagcgatt gcctgcgtgg catttaactt tggcggcact attaccgtct ttccatcact
     9721 ggtcagtgag ttcttcggcc tcaataacct ggcgaaaaac tacggtgtga tttacctcgg
     9781 tttcggtatc ggtagcattt ttggttcgat tatcgcctca ctgtttggcg gtttctatgt
     9841 gaccttctac gtgattttcg ccctgctgat tctgtcattg gcgctttcta cgacgattcg
     9901 tcagccagag cagaaaatgt tgcgtgaggc gcatggctcc ctttaatgct cagccgcgag
     9961 ctactgggct tcagcttccg tgtgcatcaa acgcatcaac gaaatgtata aacaatgact
    10021 cgtaaaattt gggccagtta actggccctt tttactgctt tttgtaacgc taaagtctct
    10081 tttcaaactt gcatttttgt aaatttgtgc ttcatgcaca ctctttcccc acactttttc
    10141 gctttgctgt ggtctactta ttcgcgcgtg tagattttac ttatctgact acctccgcac
    10201 ttttcccctg ccgggcctga aaagccgcta agcagggtgt tatcacctgt ttgtccaggg
    10261 tttgtttgca tgagatacat caaatcgatt acacagcaga agctgagctt tttgcttgca
    10321 atctatattg gcctttttat gaatggcgcg gttttttatc gccgcttcgg cagctatgcg
    10381 cacgatttta ccgtctggaa aggcgtttct gctgttgttg aactggccgc caccgtactg
    10441 gtgaccttct ttttactacg tcttctatcg ctgtttggcc gccgcagctg gcgtattctg
    10501 gcgtcgctgg tggtgctctt ttccgcaggt gccagctatt acatgacctt ccttaatgtg
    10561 gtcattggtt atggcatcat cgcttccgcc atgaccaccg atatcgacct gtcaaaagaa
    10621 gttgttggtc tgaactttat tctctggtta atcgccgtta gtgcattgcc tcttatcctt
    10681 atctggaata accgctgtcg ctacaccttg ctccgacaac tgcgaacccc agggcagcgt
    10741 attcgcagcc tggtggtcgt cgtactggcg ggtattatgg tttgggcacc gattcgtttg
    10801 ctggatatcc agcagaagaa agtggagagg gcgaccggcg ttgatttgcc gagttatggc
    10861 ggtgtcgtag cgaactctta tctgccatca aactggcttt ctgcgttggg gctgtatgcc
    10921 tgggcgcggg tcgatgaatc ttccgataat aattcattgc ttaatccggc gaagaagttt
    10981 acataccagg ctccacaaaa cgttgatgat acttatgtcg tgtttatcat cggtgaaacc
    11041 acgcgttggg accatatggg tattttcggc tatgagcgta ataccacgcc gaaactggcc
    11101 caggagaaaa atctggcggc gttccgtggt tactcctgtg ataccgcaac caaactctca
    11161 ctgcgttgca tgtttgtacg tcaggggggc gcggaagata atccgcagcg cacattaaaa
    11221 gaacagaaca ttttcgcggt tctgaagcag ttaggattca gttctgacct ctacgctatg
    11281 cagagcgaaa tgtggttcta cagcaacacg atggcagaca acattgctta tcgtgagcag
    11341 attggtgcgg agccacgtaa tcgtggcaag ccggtagatg atatgttgct ggtagacgaa
    11401 atgcagcaat cgctagggcg caacccggat ggtaagcatc tgatcattct gcataccaaa
    11461 ggttcgcatt ttaactacac ccagcgttat ccgcgtagct tcgcgcagtg gaagccggaa
    11521 tgtattggtg ttgatagcgg ctgtaccaaa gcgcagatga tcaactccta tgacaactcg
    11581 gtgacctatg tggatcactt tatctccagc gtgattgatc aggttcgcga taagaaagcg
    11641 attgtgttct acgcagctga ccacggtgag tcaattaatg aacgcgagca cctgcacggc
    11701 acgccgcgtg aactggcacc gccggagcag ttccgcgtac cgatgatggt ctggatgtca
    11761 gataaatatc tggaaaatcc ggccaatgcg caggcgtttg cgcagctgaa aaaagaagcc
    11821 gacatgaaag tgccacgccg tcacgtagag ctgtacgaca ccatcatggg ttgtcttggc
    11881 tatacttcac cggatggtgg aattaacgaa aacaacaact ggtgtcacat cccgcagaca
    11941 aaagtggcag cggctaacta agcggcctgc tgactttctc gccgatcaaa aggcattttg
    12001 ctattaaggg attgacgagg gcgtatctgc gcagtaagat gcgccccgca ttcggtgatt
    12061 ggcgcagcct ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg aatcctctat
    12121 caccgaccaa attcgaaaag cctgctcagc gagcaggttt ttttgcattt gtagctcatg
    12181 aggatgagaa cctccggggg cagggaggct cgactcgacg taggcctgat aagacgcgcc
    12241 agcgtcgcat caggcaacgg caccgcactt attgtcggat gcggcgcgac cgccttattc
    12301 gaccaacaca ccacacattt tcctgccaac tttgcgcccc atatctcact atccattaac
    12361 ggatttgtga cagaaaccat tgtctttttt tatatttgct taaatctttt ttgcgttact
    12421 tatggctggc gctggcattt tccgctgtgc gttttagcgt tcatggtatt aatcgctcag
    12481 atatccacct tttcctcaga tttttttcat taaatgcata aaacttaatc accaaatttt
    12541 gctaaatatt aagctgattg ttctggttat gttggagtag cacaaatttc tctgctgcaa
    12601 atagctgttt gaagtttttt taatctttgt ttgtggattc tcaccgttgg tgtaaatccc
    12661 ggttggctgt attgacgttt tcacattctg ttgacagatt gtaggtcacg aggggcattt
    12721 tatggaggat ccgcactgtt acactgatgt taattagtac ggcatcccca cctcataacg
    12781 ttgacccgac cgggcaaaaa acaaaaaagg tcaggcagcg acaacccact gcaaagggtt
    12841 aaaacaacaa acatcacaat tggagcagaa taatgcgtat ttccttgaaa aagtcaggga
    12901 tgctgaagct tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt caggctaaaa
    12961 ctctggttta ttgctcagaa ggatctccgg aagggtttaa cccgcagctg tttacctccg
    13021 gcaccaccta tgacgcctct tccgtcccgc tttataaccg cctggttgaa tttaaaatcg
    13081 gcaccaccga agtgatcccg ggcctcgctg aaaagtggga agtcagcgaa gacggtaaaa
    13141 cctatacctt ccatctgcgt aaaggtgtga agtggcacga caataaagaa ttcaaaccga
    13201 cgcgcgaaat gaacgccgat gacgtggtgt tctcgttcga tcgtcagaaa aacgcgcaaa
    13261 acccgtacca taaagtttct ggcggcagct acgaatactt cgaaggtatg ggcttgccgg
    13321 agctgatcag cgaagtgaaa aaggtggacg ataacaccgt tcagtttgtg ctgactcgac
    13381 cggaagcgcc gttcctcgct gacctggcaa tggacttcgc ctctattctg tcaaaagaat
    13441 atgctgacgc gatgatgaaa gccggtacac cggaaaaact ggacctcaac ccaatcggaa
    13501 ccggtccgtt ccagttacag cagtaccaaa aagattcccg tatccgctac aaagcgtttg
    13561 atggctactg gggcaccaaa ccgcagatcg atacgctggt cttctctatt acccctgatg
    13621 cttccgtgcg ttacgcgaaa ttgcagaaga atgaatgcca ggtgatgccg tacccgaacc
    13681 cggcagacat cgctcgcatg aagcaggata aatccatcaa cctgatggaa atgccggggc
    13741 tgaacgtcgg ctacctctcg tataacgtgc agaaaaaacc gctggatgac gtgaaagttc
    13801 gccaggcact gacctacgcg gtgaacaaag acgcgatcat caaagcggtt tatcagggcg
    13861 cgggtgtatc agcgaaaaac ctgatcccgc caaccatgtg gggctataac gacgacgttc
    13921 aggattacac ctacgatcct gaaaaagcga aagccttgct gaaagaagcg ggcctggaaa
    13981 aaggtttctc catcgacctg tgggcgatgc cggtacaacg tccgtataac ccgaacgctc
    14041 gccgcatggc ggagatgatt caggcagact gggcaaaagt gggcgtgcag gccaaaatcg
    14101 tcacctacga atggggtgag tacctcaagc gtgcgaaaga tggcgaacat cagacggtaa
    14161 tgatgggctg gactggcgat aacggggacc cggataactt cttcgccacc ctgttcagct
    14221 gcgccgcctc tgaacaaggt tctaactact caaaatggtg ctacaaaccg tttgaagatc
    14281 tgattcaacc ggcgcgtgct accgacgacc ataacaaacg cgttgaactg tacaagcaag
    14341 cgcaggtggt gatgcacgat caggctccgg cactgatcat cgctcactcc accgtgtttg
    14401 aatcggtacg caaagaagtc aaaggctatg tggttgatcc attaggcaaa catcacttcg
    14461 aaaacgtctc tatcgaataa ttaaaagcca tacaagactg atggcaaagg caaaactgcc
    14521 tgatgtgctc cgcttatcag gcctacgaaa attctgcaat gtattgaatt tgcacgattt
    14581 tgtaggccgg ataaggcgtt aacgccgcat ccggcataaa caaagcgcac tttgtcaaca
    14641 atctgtatac ccggtggcgc tgtgcctctg cgtgcagcgc catccggcag caatacttca
    14701 ttctctgtcc gacaagggcg ggaattgatt tgtgagcaat acagacacgc agttccaggc
    14761 tgcgggtcac tatagagaat ccgggttatg ttgcagttta ttctccgacg tttgggactc
    14821 gtcatcccca cgtttatcgg tattaccctt ctcacatttg cctttgtcca catgatcccg
    14881 ggcgatccgg tgatgatcat ggcaggcgaa cgtgggatct cccctgagcg tcacgtgcag
    14941 ctgctggctg aactcggctt agataaaccg atgtggcagc agtatctcca ttacatttgg
    15001 ggcgtaatgc acggcgatct aggcatttca atgaaaagcc gaattccagt atgggaagag
    15061 ttcgtgccgc gctttcaggc cacgctggaa cttggcgtct gcgcgatgat ttttgctacg
    15121 gcagtcggta ttccggttgg tgtgctggct gcggttaaac gcggttccat tttcgatcac
    15181 actgcagttg gcctggcgct gaccggctac tcgatgccga tcttctggtg gggcatgatg
    15241 ctgatcatgc tggtttcggt gcactggaac ctgacgcccg tctccggtcg cgtgagcgat
    15301 atggtgttcc tcgatgactc caatccgtta accggtttta tgctaatcga caccgccatc
    15361 tggggtgaag acggcaactt tatcgatgcc gtcgcccata tgatcttgcc cgccattgtg
    15421 ctgggtacta ttccgctggc ggtcattgtg cgtatgacac gctcctcgat gctggaagtg
    15481 ctgggcgagg attacatccg caccgcgcgc gccaaagggc taacccgcat gcgggtgatt
    15541 atcgtccatg cgctgcgtaa cgcgatgctg ccggtagtga ccgttatcgg cctccaggtg
    15601 ggaacattgc tggcgggggc gattctgacc gaaaccatct tctcgtggcc cggtctgggg
    15661 cgctggttga ttgacgcact gcaacgccgc gactatccgg tagtgcaggg cggcgtattg
    15721 ctggtggcga cgatgattat cctcgtcaac ttgctggtcg atctgctgta cggcgtggtg
    15781 aacccgcgta ttcgtcataa gaagtaaggg gacatcatgt cacaggttac tgaaaataaa
    15841 gtgattagcg caccggtgcc gatgaccccg ttacaggagt tctggcacta ttttaaacgc
    15901 aacaaaggcg cggtcgtcgg gctggtttac gtcgccatcg tgctgttcat cgcgatcttt
    15961 gccaactgga ttgcgcctta taacccggcg gatcagttcc gcgatgcgct gctcgccccg
    16021 ccagcctggc aggaaggcgg cagtatggca cacttgctgg gcaccgatga cgtcggtcgt
    16081 gatgtgctgt cgcggctgat gtacggcgcg cgtttatcgc tgctggttgg ctgtctggtg
    16141 gtggtgttgt cgctgattat gggcgttatt ctcggcctga tcgccggtta cttcggcggc
    16201 ctggttgata acattatcat gcgcgtggtc gatatcatgc tggcgctgcc aagtctgctg
    16261 ttggcactgg tgctggtggc gattttcggc ccgtcgattg gtaacgccgc gctggcgctg
    16321 accttcgttg ccttgccgca ctatgtgcgc ttaacccgcg ccgccgtgct ggtggaggtg
    16381 aatcgcgatt acgtcaccgc ctctcgcgtg gcaggtgccg gggcgatgcg ccagatgttt
    16441 attaacatct tcccgaactg ccttgcgccg ctgattgttc aggcgtcgct cggtttctct
    16501 aacgccattc tcgatatggc tgctcttggt ttcctcggca tgggggcaca gccgccaacg
    16561 cctgagtggg gcaccatgct ctccgacgtg ttgcagttcg cgcaaagcgc ctggtgggtc
    16621 gtgaccttcc ctggtctggc gatcctgttg acggtgctgg catttaacct gatgggtgac
    16681 ggtctgcgtg acgcgctcga tcccaaactg aagcagtaag aggttcgaga tggcgttatt
    16741 aaatgtagat aaattatcgg tgcatttcgg cgacgaaagc gcaccgttcc gcgccgtaga
    16801 ccgcatcagc tacagcgtaa aacagggcga agtggtcggg attgtgggtg agtccggctc
    16861 cggtaagtcg gtcagttcac tggcgattat ggggctgatt gattatccgg gccgcgtaat
    16921 ggcagaaaaa ctggagttta acggccagga tttgcagcgt atctcagaaa aagagcgccg
    16981 caacctggtg ggtgccgaag tggcgatgat cttccaggac ccgatgacca gccttaaccc
    17041 atgctacacc gtgggtttcc agattatgga agcgattaaa gtgcatcagg gcggcaacaa
    17101 aagtacccgc cgtcagcggg cgattgacct gctgaatcag gtcggtattc ccgatccggc
    17161 ttcgcgtctg gatgtttacc cgcatcagct ttccggcggc atgagccagc gcgtgatgat
    17221 cgccatggcg attgcctgtc ggccaaaact gctgattgcc gatgaaccga ccaccgcgct
    17281 ggacgtgacc attcaggcgc aaatcatcga actactgctg gagctacagc agaaagagaa
    17341 catggcgctg gtgttaatta cccatgacct ggcgctggtg gcggaagctg cgcataaaat
    17401 cattgtgatg tatgcaggtc aggtagtaga aacgggcgat gcacacgcca tcttccatgc
    17461 gccgcgtcac ccgtatactc aggcattgct gcgtgcgctg ccagaatttg ctcaggacaa
    17521 agaacgtctg gcgtcgttgc caggtgttgt tcccggcaag tatgaccgcc cgaacggctg
    17581 cctgcttaac ccgcgctgcc cctatgccac tgacagatgt cgcgctgaag aaccggcgct
    17641 gaatatgctc gctgatgggc gtcagtccaa atgccattac ccacttgatg atgccgggag
    17701 gcccacacta tgagtacgca agaggccacc tcgcaacaac cgctgttgca ggctatcgac
    17761 ctgaaaaaac attatccggt gaagaaaggc atgttcgtgc cggaacgtct ggtgaaggcg
    17821 ctggacggcg tttcgtttaa ccttgaacgt ggcaaaacgc tggcagtagt gggcgaatct
    17881 ggctgcggta aatcgaccct cggtcggttg ctgacgatga tcgaaacgcc caccggcggc
    17941 gagctgtatt atcaggggca ggatctgctc aagcacgatc cgcaggcgca gaagctgcgt
    18001 cggcagaaaa tccagatcgt cttccagaat ccctacggtt cgctgaatcc gcgtaagaaa
    18061 gttgggcaaa ttctcgaaga gccgctgctt attaacacca gcttaagcaa agaacagcgt
    18121 cgggaaaaag ccctgtcgat gatggcaaaa gtcggcctga aaaccgagca ctatgaccgc
    18181 tatccgcaca tgttttccgg cggacaacgt cagcgtatcg ctattgcccg tggtctgatg
    18241 ctcgacccgg atgtggtgat tgccgatgag ccggtttctg cgctggacgt ctcagtgcgt
    18301 gcgcaggtgc tgaatctgat gatggatttg cagcaggagt tggggctttc ttatgtcttt
    18361 atctcccacg acctgtcggt tgtggagcac attgctgatg aagtgatggt gatgtaccta
    18421 ggccgctgcg tggagaaagg aacgaaggac caaatcttca ataacccgcg ccatccgtac
    18481 acccaggcgc tgctctccgc gacgccacgc ctgaacccgg acgatcgtcg tgaacgcatc
    18541 aaactcaccg gtgaactgcc aagcccgctg aatcctccgc cgggttgcgc cttcaacgcc
    18601 cgctgtcgtc ggcgcttcgg cccctgcacc cagttgcagc cgcagctaaa agactacgga
    18661 ggtcaactgg tagcttgttt tgctgttgat caggatgaaa atccgcagcg ttaactcaca
    18721 aaaccggggc catgcgctcc ggtttactca atcagcttca acagtggcga caagcatagc
    18781 aaaatgccgt aaagcagaat cagccaggct ttaaatccgc gaagtttttc cagttgtgcg
    18841 actttataga tcaggaaaaa cggaatgagg cacgacacaa tgccatataa cgggcttccc
    18901 aactgaaaga acaccagcac tgatacacga aacgaaaccc aaatcgtcaa cgtaatgacg
    18961 ataaaagcgc agatcgccag agtcagcatg cgtgagttaa ttttcttggt atcaataatt
    19021 cggcttaaca gattgagaat aatgccttta atagcctcgt ggaaaccgag ataaatgcca
    19081 aagaatgcgg tcagtacggc aaagatattc agcaccgtag aggtgatatg aatgatatgt
    19141 ccagggatca cctgcgcggc cagtgccagt gccgagatat tttgttcaaa ggcagaaacg
    19201 gcttcttcgt ggctaattga gaaggtaaac gaaaaggcaa aaaacaggat caccgcgatg
    19261 agcgtgatat aactaattcg gtgggtacgc agcgcgagcc gggttgccag tactttatct
    19321 gcttcccgtt tacgataggc aatattcatt gggttaagta cctgaataaa taccgcagaa
    19381 aagaagcaaa atggaatggt aagcaggaca tcgcggaaaa agacggaggc ttgcgggaag
    19441 gcggttatat tggcgaaatt ccagtgcggg atcatcgcaa aaccgaacac gacaataatc
    19501 cctactttga ccaccaccat tggcccagaa atcttaaaca gtaatcgttc accaccagac
    19561 gcaatcgcta tcagtacggc gaaaatagcg actttataaa gtagagattg tgaaagatcg
    19621 gcatcggtta aaccgaaggt tttcaggtac gaggcgctgt cgaaaaccac ggagagagag
    19681 tagataaaaa tcccgtggat aatcatcaaa aagtagataa ccccgaggaa aattccccag
    19741 ttcttcccca ggtaatgact gataatatcg gtgtagtcat tacaggaatc gctttcagaa
    19801 agggttttta aataaatgtc ctgcactacc caggtggcag gataagcaat gatcgctgcg
    19861 gtaataaata cccaaattcc cttcaagcca atttgtactg gcatcagcac ggttccggca
    19921 ccaatcgcca tgcctatgca taataaaacc cagccgaagt cgtagcgtgt aaacggcaat
    19981 ttctgattgt gtttcgatag tgtgttgtgc tgcataaagt cctgtccaga aagaagtcgg
    20041 cctcgcaaaa atgaataaac ccagtttctc ttgcgcaaca ggtggagaag tcgaatacgc
    20101 agtatgtaaa aaatgaggca acagattcac tggggaggtc gcgaggaggc gcaattctac
    20161 aaaattgcgc atttcactac cagtctggag tcagcccgga tatgaactgc aactattgca
    20221 aataaagaaa ttaagctgga aggcatcgcg caatgaggaa gattgccggg tgctgacagt
    20281 aaaaagcaat cgtaaaatca gcg
//
LOCUS       DADMGC010000073        19900 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.073, whole genome shotgun sequence.
ACCESSION   DADMGC010000073 DADMGC010000000
VERSION     DADMGC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19900
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.073"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            7..375
                     /locus_tag="JNZ73_002510"
     CDS             7..375
                     /locus_tag="JNZ73_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732754.1"
                     /translation="MFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSER
                     PIWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPIRCAFTGALTLYYYFIGFF
                     GILAAIAITLVWQRHTRFFH"
     gene            complement(411..1367)
                     /locus_tag="JNZ73_002511"
     CDS             complement(411..1367)
                     /locus_tag="JNZ73_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAY8732755.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1367..2608)
                     /gene="clsB"
                     /locus_tag="JNZ73_002512"
     CDS             complement(1367..2608)
                     /gene="clsB"
                     /locus_tag="JNZ73_002512"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HAY8732756.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2605..3366)
                     /locus_tag="JNZ73_002513"
     CDS             complement(2605..3366)
                     /locus_tag="JNZ73_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY8732757.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3499..3909
                     /locus_tag="JNZ73_002514"
     CDS             3499..3909
                     /locus_tag="JNZ73_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732758.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3871..4977)
                     /locus_tag="JNZ73_002515"
     CDS             complement(3871..4977)
                     /locus_tag="JNZ73_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8732759.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(4988..6121)
                     /locus_tag="JNZ73_002516"
     CDS             complement(4988..6121)
                     /locus_tag="JNZ73_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8732760.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6114..7850)
                     /locus_tag="JNZ73_002517"
     CDS             complement(6114..7850)
                     /locus_tag="JNZ73_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8732761.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7843..8841)
                     /gene="hlyD"
                     /locus_tag="JNZ73_002518"
     CDS             complement(7843..8841)
                     /gene="hlyD"
                     /locus_tag="JNZ73_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HAY8732762.1"
                     /translation="MMKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(8841..9512)
                     /gene="cecR"
                     /locus_tag="JNZ73_002519"
     CDS             complement(8841..9512)
                     /gene="cecR"
                     /locus_tag="JNZ73_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HAY8732763.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHSHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9741..10928
                     /gene="rhlE"
                     /locus_tag="JNZ73_002520"
                     /pseudo
     CDS             9741..10928
                     /gene="rhlE"
                     /locus_tag="JNZ73_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(11135..11617)
                     /gene="ybiA"
                     /locus_tag="JNZ73_002521"
     CDS             complement(11135..11617)
                     /gene="ybiA"
                     /locus_tag="JNZ73_002521"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HAY8732764.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            11737..13887
                     /gene="dinG"
                     /locus_tag="JNZ73_002522"
     CDS             11737..13887
                     /gene="dinG"
                     /locus_tag="JNZ73_002522"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HAY8732765.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            13915..14877
                     /gene="ybiB"
                     /locus_tag="JNZ73_002523"
     CDS             13915..14877
                     /gene="ybiB"
                     /locus_tag="JNZ73_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HAY8732766.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            15018..16103
                     /locus_tag="JNZ73_002524"
     CDS             15018..16103
                     /locus_tag="JNZ73_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HAY8732767.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHKETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(16332..16592)
                     /locus_tag="JNZ73_002525"
     CDS             complement(16332..16592)
                     /locus_tag="JNZ73_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY8732768.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(16857..17123)
                     /gene="ybiI"
                     /locus_tag="JNZ73_002526"
     CDS             complement(16857..17123)
                     /gene="ybiI"
                     /locus_tag="JNZ73_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HAY8732769.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(17197..17910)
                     /gene="ybiX"
                     /locus_tag="JNZ73_002527"
     CDS             complement(17197..17910)
                     /gene="ybiX"
                     /locus_tag="JNZ73_002527"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HAY8732770.1"
                     /translation="MWGAGPTFWRTCMMYHIPGVLSPQDVAHFREQLEQAEWVDGRVT
                     TGAQGAQVKNNQQVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNN
                     ETYGFHVDGAVRSHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGD
                     LVLYPSSSLHCVTPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENE
                     EILSLLNLYHNLLREWSEI"
     gene            complement(17916..19900)
                     /locus_tag="JNZ73_002528"
                     /pseudo
     CDS             complement(17916..19900)
                     /locus_tag="JNZ73_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         4613 a         5230 c         5374 g         4683 t
ORIGIN      
        1 ggcgcgatgt ttttaatcag tatgctggcc ggtttattat ttaatgttga tcctggttct
       61 caccgtttta tcattatgat gacgttgaca gggttggccc tggtaatcat cgtgaatgcg
      121 gcattaatga gtgaacggcc catttgggta ataagttgct taatgattgt gttatggcca
      181 ggcattatct cgcatggacg aaataagctc cttgaactgg caggaaaatg ccatagtgaa
      241 gagttgtgga gtccgattcg ttgcgctttt acaggtgcat taacactcta ttactatttt
      301 atcggcttct ttgggatact tgccgcgata gctataacgc ttgtctggca aaggcatacg
      361 cgtttttttc attagtcgta aatgcagata ctaaagaact taactaaact tcacatcgcc
      421 gcttcatttt tcgcccgtag cttcttcgct tggctttcca gtagcaggta acagatcagc
      481 gccagcagca gcgggataaa gtaatacagg acgcgataag cgagcagggc ggcgataatg
      541 ctccctttcg aggtatgctc cccagccagt agtgcgataa acaccgcttc cagcacaccg
      601 atccccgccg gaatatgcac gatgacgcca gcaatactac taaccagtaa cacgcccagt
      661 acaaagaaat agttcacgct ttgaccaagt aacagccaga taatcgcccc cattaccatc
      721 cagttaacgc tggaaatcag catctgagcg agggcgaatt tccatgaagg cagcaccagc
      781 ttttgccctt tgatggtcat atggcggtgc ttcgcgaaag cgcaaaacca caaataaacc
      841 gcgataatca tcagtaagcc aatgccgaga atacgcagcg tagtttgatc gacataccag
      901 tgatccggca actccaccac gcctgcggta aagataatcc ctgccagtaa aatatagccc
      961 agccagttgg tggtaatact gagcgaggaa atccgcgtaa tagtgctgcc cggtaaccct
     1021 agtcgggagt acaaacgata gcgcatacca atgccgccga cccaggtact gagcgtcagg
     1081 ttgaaggcgt agcagataaa cgacaccagc atcacctggc gcttcgccag tttgtgaccg
     1141 cagtaaaagc gggcgagcag gtcatagcag ccgtaaatca gatagctgac gaccaccagc
     1201 ccgaccgcac taagcagcgc aacgcgattg tagtcgcgga tgaccttcca gacctcttcc
     1261 cagtccactt ttttggcgta gaccaccagc aacacaatca ccgcgataaa aaacagccag
     1321 gtgaggatct tctttgctaa gcgccagcgc gggtgtgatt tactcatcag ggttttaccc
     1381 ccgtgttttc agtttctacc cgatcctgcg tttccattgt cggttgtgcg gggggatcaa
     1441 cctgcgtcag acgtggtgtg tgtgccggaa gccagccaac cagcgccggg aagtggcgta
     1501 aaaagtggaa cgccagcacg cttttggtca ggttccacca ggtgcgtttg ggcagcatgg
     1561 tttcatccac ctgctgacaa tctgcggcaa taatgccgtt cagattatcg cgcagcgtct
     1621 ggttaaaatg acgatcgtgg atgatgacat ttgcttcgag attcagtgac aaactgagcg
     1681 gatcgagatt actggaccct actgtcgccc agtgatcgtc catcaatgcc actttgccgt
     1741 ggagcgggcg gcggcggtac tcaaaaacct gaacgccgcc tttaaccaga tagttataca
     1801 gcaagcgcgc accgactctg acaatcggca tatccggttc gccctgaatg atcagtttga
     1861 tccgcacccc gcgccgtgcc gctttacgca aggcgtgtaa aaatcgatag ccggggaaga
     1921 agtaggcgtt ggcgataatc acttcccgcc gcgcctgagt gagcattttc agataatggc
     1981 gttcaatatc atcgcgatgt tcttcgttat cgcgccagac cagcaatacc tgcgcttctc
     2041 ccggctggcg gttctcttcc gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct
     2101 gtccaggcag gttttccagc tcaaactgga gaatatcttc gacaatcggc ccttcaaggc
     2161 gtaccgcgta atcctgttta gcctctggac cgtagctgga catatgctcg gcggagtaat
     2221 tcagcccgcc aataaaggcg atacgcgcgt cgatcaccac aattttgcga tgcatccggc
     2281 gaaacacatt ggtgcgcata ccaaaaaggc gagggcgggg atcgtagtag cggaacacta
     2341 cgccagctgc cgtcagttca ttgacaaact catcgctgag atccggcgaa ccgtagccat
     2401 ccagcaagac ttccgcttta accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct
     2461 gtttgccgac gttatcctca aaccagataa acgtttcaag aatgatgcgt tcttgtgcct
     2521 cgccaatcgc cttaaacacc gcgggataat attgctcgcc gttttccagc aactggatct
     2581 tattgccttc gcgccagcta catttcataa atgaatctcc gcacttaaag gggcatgatc
     2641 agaaaggtgt cgccatgtcc gcagcggcaa cgcggttggc gcgctggcgc tggcattttt
     2701 gacgtagatc ctgtccagtc gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg
     2761 tccgtgggcg cgggtaaaaa tctcatccag tccggcctgc acttttaacg gatgattagc
     2821 tttttgccgc cagtcattga aatcacccgc caccaatacc ggttcgccgt ccggtagctc
     2881 attcacccat tcggcgagca tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag
     2941 atgtacgcac atcacatgaa tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag
     3001 cacgccgcgc ttttccgcac catcgaccga aacatcgcga ttctcataat gttcaatggg
     3061 ataacgcgac agtacggcgt tgccgtgatg cccttccggg tatacggcat tgcgaccgta
     3121 ggcaaaatcg ctccacatag tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt
     3181 ttccacatgc agcggatgaa cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc
     3241 ggcgctgacg gtacgcacgg catcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc
     3301 ggtaaagcct ttgtgaatgt tgatggtgag caccttgaac gaaaattgtt gtgtttgatc
     3361 gggcataatt ttcctgtctt tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa
     3421 aaaggtgcgg tcttacggaa ttttccgtaa agttcggtac tctgagtaag tagagataaa
     3481 ttcttcagga gagaagccat gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt
     3541 tgctggcaga ttatgttgca tttactggta gtggcgctgc tggtggtggg ctggatgagt
     3601 aagactctgg ttcacgtcgg cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg
     3661 atgctggtgt ttcagcgcca ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa
     3721 gagctgacca cgacctggta ttttggcgca gcgctgattg tgctgtggct gttgtcccgc
     3781 gtactggaaa acaacttttt gctggcaatt gcagggctgg cgatccttgc tggcccggcg
     3841 gtagtgtctt tgctggcgaa agataagaag ttacatcacc ttacgtctaa acatcgcgta
     3901 cgccgctgac cctgtcgtgg ccgttatcac cagtagcggc cacaaactat tccacacaat
     3961 atccagactc gcatccttca aataaatctg cttggtaatg tccgtaaagt ggcgaatagg
     4021 gttaatccac gtcagatttt gcagccacac tggcatgttt tccaccggcg aaacgtaacc
     4081 ggaaaggaga atggcgggca tcataaaaac aaacacgccg ataaacgcct gctgttgtgt
     4141 cgagcagagt gatgaaatca acagaccgaa tcccaccagc gataaaccgt aaatcaccat
     4201 cgtaaagtag aacagcgcta gcgatccggc gaaggggatt tgatacgccc agataccaat
     4261 cgccagcaca atggtggcct ggaaggtggc gacaattaac gccggtacgg ctttgccgat
     4321 gaagatctgc caggtggtga gcggcgaaac cagtagctga tcgagcgtac cttgttcacg
     4381 ttcgcgggcg acggaaagtg aagtgacgat cattacgccg atagtggtga tcatggcgat
     4441 cagcgacggc accacaaacc atttgtagtc gagattcggg ttataccagt tgcgtaccac
     4501 cagctcgctg ttgttgggtt tcggttttcc ttccagcagc tcctgctgat aatttttgac
     4561 gatctgttgc aggtagttgg cggcaatttg cgcactgttg gagttacgcc cgtcgaggat
     4621 caactgcaac ggcgcggtct ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg
     4681 caccagtagt aacgcctttt gtgtgtcgat ggtcgggcgg atctcctgtg ggcttttcag
     4741 cagcagcaca tgtgtaaagg cgctggcgcg ggcaaaacgt tgagtaagtt ccaccgaatg
     4801 ctcgccgtta tcttcatcgt agatggcgat ggtggcgtta gtcacttcca gcgtggcggc
     4861 gaacgggaac aggatcacct gaattagcac gggtaaaatc agaatcgcgc gggtttgtgg
     4921 ttcgcgcagc aacgactgca actctttgcg gattaacgtc cataagcgat gaaacatgct
     4981 cttctcccta atccagccga cgtttggttt tcagccacgt caggccgata aacatcaccg
     5041 ccgaagcgat caaaaacagc acgtttacca ccagcaccac tggaatattc ccggcgagga
     5101 acaggctttg cagggtgctg acgaaataac gagcgggaat aatgtacgtc accgcgcgga
     5161 tcaccgcggg catactgtcg atctgaaaaa taaagccgga aagcataatc gacggcagaa
     5221 aagcggcgtt cagggcgacc tgagcggcat tgaactggtt gcgggtaatc gtggaaatca
     5281 gcagccccat tcccagggta ctgagtaaaa acaggctgga gataaaaaac agaatcagca
     5341 gcgaaccgcg atacggcacg ccgagaataa acactgacac cagcatacac agcaacatcg
     5401 ccagcatccc gagaaagtaa taagggatca gcttacacag cagcagttcc gtgcgggtaa
     5461 tctccgtaga gagcagagcc tccatggtgc cgcgttccca ttctcgcgcc accaccagcg
     5521 aggtgagaat cgcgccgatg accgtcatga taatggtcac cgcacccggg ataataaagt
     5581 gctggcttat cgccgccggg ttaaaccagt agcgggtttg tacatcaata agcggttcaa
     5641 aagtctgccc gttgtcctcc gctcgctgca tttgccagat ctgccagatc ccttcgacat
     5701 acccctgcac aaagttagcg gtattcggct cactgccgtc ggtgatcacc tgaatcggtg
     5761 cggtggcgtt ggcgcgctcc atctgttcgg cgaaatccac gggaataacc accagaccgc
     5821 gaattttccc cgactgcatt ttggcgatca gttcctgacg gttatcgctg atggtggcgt
     5881 cgatgtaggg cgaaccggtc atggtgtggg tgaaatccag cgccgcttcg ctacgctgtt
     5941 ccagcaaaat cccgacccgc agcttgctgg agtcgaggtt aatgccgtaa ccaaaaataa
     6001 acagcagtag cagtgggatc actaccgcaa tcagccagct actcggatcg cgaacgatct
     6061 gccgcgtctc tttgacgcac agcgcccgca cgcgatgcca ggaaaggatc gggttactca
     6121 ttgctatgct ccttatccca gtcgtggatc aactgaataa aggcttgctc catggtggga
     6181 tcgggttgct catcgttagc tgactgtgct ttcaaatcgt ccggcgtacc gctggcgatt
     6241 aatttcccac ggtacaccag gccgatgcgg tcgcaatatt ccgcttcatc cataaagtgg
     6301 gtggtgacca tcaccgtgac gcctttctct accatgctgt tgatgtgcag ccaaaattca
     6361 cggcgggtga gggggtcaac gccggaagtc ggttcgtcga gaaagagaat atccggttca
     6421 tgcatcagcg aacaggccag cgccagccgc tgtttaaaac ctaatggcag ttcatcggtg
     6481 gcgtgtgagg caatactttt caggccaaat gcctcgctca tacgggagat tttttcgttc
     6541 tgcgcccgac cgcgtaagcc atacacacca gagaaaaagc gtaaattctg ttcgaccgtc
     6601 aggttgccgt agagcgaaaa tttttgcgcc atatagccga gatgctggcg cgctttaccg
     6661 gaactctctt tcagatccat ccccagcacc agcgcctggc cggaagtcgg caccagcaaa
     6721 ccgcacatca tcttaaaggt ggtcgattta cccgcgccgt ttggccccag caaaccaaaa
     6781 atctccccac gtttaacggc aaagttgacg tgatcggtgg cggcaaaatc accaaatttc
     6841 ttggtcagtt ctttcgcttc gatcaccgtc tcgccgggtg tgccttctac cgtatgcaat
     6901 attgcaccca gcggcgattc tgaggttccg gcaccgccca gcaaatcaat aaacgcatct
     6961 tcaaaacgcg gcgtagtttc gttgatgttg atttccggca tcccgtcggc atggcgaata
     7021 tcgtctggtg tggcctcttt tttgaggatc agacgtaccg atttcccctg aatcatgccg
     7081 tcgctgacct gcggcagttt caaggcgcgt tgcaacagtt tgcggttgcc ctcgtgtgga
     7141 ctggtcatca gaaagctgcg tccggccatg gtttgtgtca gggcttttgg ttctccctga
     7201 tacagcaact cgccttcgtt catcagtaac acgtcacggc actgctcggc ttcgtcgaga
     7261 tacgaggtac tccagaggat taacatccct tcgcccgcca gctcatgcac catctgccac
     7321 agttcgcgcc gtgagatagg gtcaacgccg acgccgggtt catcgagcag caacactttc
     7381 ggttcgccca ccagggtaca ggccaggccg agtttttgtt tcatcccacc ggagagcttg
     7441 cccgccaggc gtccggtaaa cggcccaaga gacgtaaact ccagcaggcg agcaaaagtt
     7501 tgcttacgtg cctcgccggt gacgctgcgc aaatccgcgt acagattgag gttctccatc
     7561 accgtgagat cttcatacag accaaatttc tgcggcatat agccgagcac ggcgtgcagc
     7621 gcgccgtcgt ttttgatcgg atcaaagcca atcaccgtgg cactgccgct gtcgggtttc
     7681 agtaatcccg ccaacatccg catcagcgtg gttttacctg caccgtccgg ccccaccaac
     7741 cccgtcacat aaccggcgtg aatggtacaa tcgagcggcg cgacggcggg cttgtccatg
     7801 cccggaaagc gtttttccag gccgttcagc gtgataacgg catcattcat gtcctgcctc
     7861 gtcaccgaat tgtaccgtca ctggcattcc ctggcgtaac gcatcatcgg cgtcggtcac
     7921 cacaatacgc aggcgataga cgaggtcggt acgcagatcc ggtgtttcga cggttttcgg
     7981 ggtaaattca gcagtcggcg aaacgaaacc aatctgcccg tgatacggct tgtccgggcg
     8041 accatcggta taaagcagca ctttgcgccc cggctgggcc tggtcaagat tccgttcatc
     8101 aacataagcg cgcacccaca ccggacgcgt tagtgaaacg gtaaacaccg tgccaccttc
     8161 attgagaacc gtgcctggct ctaccgcgcg cgttaacagc gtgccatcag acggtgctat
     8221 caacgttgag tcctgtaaat tcagctccgc ctgcgccagt tgcgcctgcg cctgttcgag
     8281 gctggctttc gcctgagcga tatcctgttc acggttaccg gaacggtact gacgcaattt
     8341 atcctgtgct gatttcagcg ttgcctgtgc ctggtcgcgc gaggagcggg cattttccag
     8401 gtcatttgcc gaaatggtgc ggcttttcca cagtccttgc tggcggttat agaagttctg
     8461 cgcatagtca taggcggctt gcgcctgttt caccgctgcg gcggcctgag cgatttcttc
     8521 atcgcgatac ccggcaagca tcagatcata ctgcgcctgt gccaccgaga cgcccgcttt
     8581 cgcctgcatc agggcaatct catacggctt gtgatccagt tcgcccagca cctggcccgc
     8641 tttgatagca tcaccttcgt ccaccgccag cgattcaacg cgccccccaa cacggaaact
     8701 aagattcacc gtacgaatat ccacgttgcc atacagcgtc aggccgttat cttggcggct
     8761 ttgataccac cagtagcctc cggcaaccac ggcggcaagt accactaccg ccagtccgat
     8821 cacgacaggt tttttcatca ctacaaactc ctttgcgata atccttgcaa aatcaggtcg
     8881 atatgacagg tcaccgtctg gttgatcagt tcggtctttt cttcatcgaa cgcggtccag
     8941 ccggtacgta acagaattgt ttctttgcca agacggaacg ccagaatctc accaatcaac
     9001 gcatgggaat gaaggatcat gcgggtgtca ttagcgtcgc agccggtcca ggcggcaatc
     9061 aggcgtgtca ggtggctgtg tagcggacta atcacctgtt cgtgcaccag gtggtaggct
     9121 gccgtgggag agagctgctc acgggagata aacttgctga ggttgacggt gtcatcctgg
     9181 gtgagcagct taatcatgtt cctgcaggcg cgaaggatca gttcacggat ggcagcccga
     9241 tcaggctgtg gttgggcgaa caagcgttcg gcttcctcgg catgcggacg gaactgctcg
     9301 ccaataaaat cggcaatcca ctgggcgcag gcgaggtaca aatcttcttt cgaaccgaag
     9361 tagtaggtga tggcagcgat attctgcccg gcctgggcgg ctatctcgcg ggtggtggcg
     9421 ttcattccat attcaccaaa ctgcgccagt gcggcagcaa tcagctgttt tttcgcctgt
     9481 tcacccttga ttgtcatggc aggattattc atcgcacagt ccattcttaa tcaaatgatt
     9541 gattaagatt atgactccat atgggagttg tccagtatgg ctaagaattt tagctacgcc
     9601 agtcacaggg ataatttatg cgctgcgtca caaaaactgc tacactccgc tccctcatga
     9661 cattgtggtt tttgtcattt tccttttcag tatctccctg aaaactacac cggtaacggt
     9721 cggggcggtt cggagtagtt atgtctttcg attctttggg tttaagccct gatatcctgc
     9781 gcgccgttgc cgagcagggt taccgtgaac ccacccctat tcagcagcag gcgatccctg
     9841 cggtgctgga aggccgcgac ctgatggcta gcgcccagac cggcaccggc aaaacagcgg
     9901 gctttacgct gccgctgttg caacacctga tcactcgcca gccgcacgcc aaagggcgtc
     9961 gtccggtacg tgcgctcatt cttaccccga cccgtgaact ggcggcacag attggcgaaa
    10021 acgtccgtga ttacagcaaa tacctgaaca ttcgttcgct ggtggtgttt ggcggtgtca
    10081 gcattaaccc acaaatgatg aagttgcgcg gcggcgttga tgtgctggtg gcaacgccgg
    10141 gacgtttgct ggacctggaa catcaaaatg cagtgaagct ggatcaggtt gaaatcctcg
    10201 tcctcgatga agctgaccgc atgctcgaca tgggctttat ccacgatatc cgtcgcgtgt
    10261 taacaaaact gccagcgaaa cgccagaacc tgctgttctc tgcgaccttc tctgacgata
    10321 ttaaagccct ggcggaaaaa ctgttgcaca acccgctgga aatcgaagtg gcacgccgca
    10381 ataccgcgtc tgatcaggtg acccagcacg ttcactttgt cgataagaaa cgcaaacgcg
    10441 aattgctgtc gcacatgatt ggtaaaggga actggcagca ggtgctggtg tttacccgta
    10501 ccaaacacgg cgctaaccat ctggctgagc agctcaataa agatggcatc cgtagtgtgg
    10561 cgatccacgg gaataaatcg caaggtgcgc gtacccgtgc gctggctgat tttaaatcgg
    10621 gtgacattcg cgtactggtg gcgaccgata tcgctgcgcg cggtctggat atcgaagagt
    10681 tgccgcacgt cgtcaactat gaactgccaa acgtaccgga agattatgtc caccgtatcg
    10741 ggcgtaccgg tcgtgcggct gctaccggtg aagcgttgtc gctggtgtgt gttgatgaac
    10801 acaaactgct gcgtgatatc gaaaaactgc tgaaaaaaga gatcccgcgc attgcgattc
    10861 cgggctatga gccggatccg tcaatcaaag ccgaaccgat ccagaacggt cgccagcaac
    10921 gtggcggcat caggctgatg aacaaacgca aaactgcctg atgcgctacg cttatcaggc
    10981 ctacgtggac tctgcaatat attgaatttg catgcttttg taggccggat aaggcgttta
    11041 cgccgcatcc ggcatttcac aacaagcact tgtcagcaat ttgagaacac gggaaaaaat
    11101 ttatctgttt taccgcccat aagccaccga gacgttactt ctctatagcc aattgttcgc
    11161 gcaactccat taaaaggtag cccagtcgat tcttgccctt accattacca ccgtctcccc
    11221 agtaagcatc gttttccgta tgctcaacca gttttgcggg cgcggttgcc agcaagagca
    11281 cacgcagttc tgcatgctgt tcgaatttag cgcgaagtgc tttacgcatc acctgttctt
    11341 taaccgactc ccagttttta cgaagtggct tggaacgatt acgccccatg cgagcggcga
    11401 ccatggggga agaaacccga cggatctctt cgcggtattt ttcatcaagg aatttttgtg
    11461 cctgaaaata gtgttcggag gtgggccagg tattcccgtc aaccttgatg ggcctggcgg
    11521 caaagttaga aaaatcaccg tagtcatcgc tggtgctgta gaaatttatg atggtgtctt
    11581 gcatgacgtg ttggatcctt tgtgctcgaa cgggcattaa accgcattat gttggtggtt
    11641 attgcgagcc gctttccaga aacagaaaaa ccattacccc tgaaaaccga aaaatgccac
    11701 aatattggct gtttatacag tatttcaggt tttctcatgg cattaaccgc cgcgcttaaa
    11761 gcgcaaattg ccgcctggta taaggcgctt caggaacaga tccccgactt tattccccgt
    11821 gcgccgcagc ggcagatgat tgcggacgtc gccaaaacgc tggccggaga agaagggcgg
    11881 catctggcga ttgaagcccc caccggcgtt gggaaaacgc tctcctattt gatccccggc
    11941 atcgccattg cccgcgaaga gcaaaaaacg ctggtggtga gtaccgccaa cgtggcgttg
    12001 caggatcaga tttacagcaa agatttaccg ctgctgaaaa agatcattcc cgatcttaaa
    12061 ttcactgccg ctttcgggcg tgggcgctac gtttgtccgc gtaatctgac ggcgctcgcc
    12121 agcactgaac ccacgcaaca ggatctgctg gcgtttcttg acgacgaact gacgccaaat
    12181 aatcaggaag agcaaaaacg ttgtgcgaag ctgaagggcg atctcgacac ttataaatgg
    12241 gatggtctgc gcgatcatac ggatatcgct atagatgatg atctctggcg tcgtttgagt
    12301 accgacaaag ccagctgcct caaccgtaac tgttactact atcgtgaatg cccgtttttt
    12361 gttactcgtc gggagattca ggaagcggaa gtggtggtgg caaaccatgc gctggtgatg
    12421 gcggcgatgg aaagcgaagc cgtactgcct gacccgaaaa atctgctgct ggtgctggac
    12481 gaaggtcatc acctgccaga tgtggcccgg gatgcgctgg agatgagcgc tgaaatcacc
    12541 gcgccctggt atcgactaca gctggacttg ttcacgaaac tggtcgctac ctgcatggag
    12601 cagtttcgcc cgaagaccat cccaccgctg gcgatccctg aacgtttgaa tgcgcattgt
    12661 gaagagttgt atgagcttat cgcctcgtta aacaacattc tcaatctcta catgcctgcc
    12721 gggcaggagg cagagcaccg ttttgcgatg ggcgaactgc ctgatgaatt gctggagatc
    12781 tgccagcggc tggcaaaact caccgagatg ctgcgtggtc tggcggagtt atttcttaac
    12841 gatttaagtg agaaaaccgg cagccacgat attgtgcgtc tgcatcggtt gattttacag
    12901 atgaaccgcg cgctggggat gtttgaggtg caaagcaaac tctggcggct ggcgtctctg
    12961 gcgcaatctt ccggtgcgcc ggtgaccaaa tgggcgacgc gggaagagcg cgaagggcag
    13021 ctacacctct ggtttcactg cgtgggaata cgtgtcagcg atcagctgga aaggctgctg
    13081 tggcgcagta ttccgcacat tattgtcacc tccgcaacct tgcgttcgct gaatagtttt
    13141 tcgcgtttgc aggagatgag tggtctgaaa gagaaagcgg gcgaccgttt tgtggcgctg
    13201 gattccccct ttaaccactg cgaacagggc aaaattgtta ttccccggat gcgctttgag
    13261 ccttccatcg acaacgaaga gcaacacatt gccgaaatgg cggccttttt ccgtgaacag
    13321 gtggagagca aaaaacatct cggtatgttg gtactgtttg ccagcggacg ggcgatgcag
    13381 cgctttctcg actatgtgac ggatttacgc ctgatgttgc tggtgcaggg cgatcagccg
    13441 cgttatcgct tagttgaact gcaccgcaaa cgcgttgcca acggtgagcg tagcgtgctg
    13501 gtgggcttac agtcatttgc cgaagggctt gatttgaaag gtgatctgct cagccaggtg
    13561 catatccaca aaatcgcttt tccgcccatc gacagcccgg tggtgatcac cgaaggggaa
    13621 tggctgaaaa gcctcaaccg ctatccgttt gaggtgcaaa gcctgccgag cgcctcgttt
    13681 aacctgattc agcaggttgg gcgactgatt cgaagccacg gttgctgggg cgaagtggtt
    13741 atctacgaca aacgcttgct gacaaaaaat tatggcaagc gactactgga tgcattaccg
    13801 gtatttccga tagagcaacc ggaagtccct gaaggtatag ttaaaaagaa agaaaaaacg
    13861 aaatccccac gccgtcggcg gcgttaatga tgtgagtcag gtaaggagtc gtaaatggac
    13921 tatcgcaaaa taattaaaga gatcgggcgc gggaaaaacc acgcgcgcga tttagaccgg
    13981 gatactgcgc gcggtctgta tgctcatatg ctcaatggtg aagtccctga ccttgagttg
    14041 ggcggcgtat tgattgcgct gcgtatcaaa ggggaagggg aagcagagat gctcggcttt
    14101 tacgaagcca tgcaaaatca caccatcaag ctgacgccgc ctgcaggcaa gccgatgccg
    14161 attgtcatcc ccagttacaa cggcgcgcgt aaacaggcta acctgacgcc attattggcg
    14221 attctgctgc ataaactcgg ttttcctgtg gtggttcacg gagttagcga agatccaact
    14281 cgcgtgctga cagaaaccat tttcgaactg atgggtatta caccaacgtt gcacggcggg
    14341 caggcgcagg cgaagctcga cgaacatcaa ccggtgttta tgcctgtcgg cgcgttttgc
    14401 ccgccgctgg aaaaacaact ggcgatgcgc tggcggatgg gcgtgcgtaa cagtgcacat
    14461 accctggcga aactggcgac gccatttgcc gaaggtgagg cactgtgtct ttccagcgtt
    14521 tcgcatccgg aatacattgg acgcgtcgcg aagttcttta gcgatatcgg cgggtgggcg
    14581 ttactaatgc atggtactga aggcgaagtg tatgctaatc cgcagcgctg cccgcagatc
    14641 aatctcattg accgtgaagg gatgcgggtg ctgtatgaaa aacaggaccc tgctggtagc
    14701 gagttactgc cacaagcaaa agatccggaa accacggcgc agtggactga gcgttgcctg
    14761 gctggcagcg aagcgattcc cgaatcgctg aaaatccaga tggcttgctg cctggtggct
    14821 acgggtgaag cggcaactat cagcgatggc ctggcgcgcg ttaatcaggc attttaattt
    14881 ttatctcagc cggatgagcc acgtcatccg gcttttaccc caccgcaata tgaaattcct
    14941 gcatctttat tgaccttccc gcgcccggcg tgcagcataa aaaaacaaca aacacataac
    15001 ataaacagga gttaaccatg gaaagtggtc atcgctttga tgctcagacg ctgcacagtt
    15061 ttattcaggc tgtatttcgt cagatgggta gcgaggaaca agaagcgaaa ttagttgccg
    15121 atcatttaat cgcggcaaac ctggcagggc atgattctca tggtattggc atgatcccaa
    15181 gctatgtgcg ttcctggagt caggggcatc tgcaaattaa ccatcatgcc aaaatcgtta
    15241 aagaggcggg ggcggcagtc acgctcgatg gcgatcgcgc attcggtcag gtcgcggcac
    15301 acgaagcgat ggcgctgggg attgagaaag cgcatcagca cggtattgct gccgtggcgc
    15361 tacataactc gcatcatatc ggccgtatcg gttactgggc ggagcagtgt tcagcggcgg
    15421 ggtttgtctc tatccacttt gttagcgtgg tcggtattcc aatggtcgcg ccgttccacg
    15481 gtcgcgacag ccgctttggc accaatccct tctgtgtggt tttccctcgt aaagataatt
    15541 tcccgctgtt gcttgattac gccaccagcg ccattgcatt tggcaaaacc cgcgtcgcct
    15601 ggcataaagg cgtccccgtg ccgccaggtt gcctgattga cgttaacggc gtgccgacga
    15661 ccaatccggc ggtaatgcag gagtcgccgt tggggtcgct gttgaccttt gccgaacata
    15721 aaggctacgc ccttgcagcg atgtgtgaaa ttcttggcgg ggcgctttct ggcggtaaaa
    15781 cgacgcataa ggaaacgttg caaaccagcc ccgatgccat tcttaactgc atgaccacta
    15841 tcatcatcaa cccggaactg ttcggcgcgc cggattgtaa cgcgcagacc gaagcctttg
    15901 ccgagtgggt gaaagcctcg ccgcatgatg acgataagcc gattttgcta ccgggcgagt
    15961 gggaagtgaa cacgcgtcgc gaacggcagg agcaggggat tccattggat gcgggaagct
    16021 ggcaggccat ttgtgatgcg gcgcggcaga ttggtatgtc ggaagagacg ttgcaggctt
    16081 tctgtcagca gttagccagc taaaaaaaag cccgtccagt ggcggacggg caaacaaggg
    16141 taacatagga tcaatgaggg ttagagcata tgcgtctgtc ggcaaacaga cagggaaata
    16201 cttgtgctgg acgtagcgta aacgcctgat ccgtccaacg gttcggattt tgtaggcctg
    16261 ataagacgcg gtagcgtcgc atcaggcatc tgttgccgga tgcggcataa acgccttatc
    16321 cgtcctacga attacttata aattaccgca gtaccgctta atttattgtt gttggtggcg
    16381 gaagtgatgc tgtatccact ggcaccggct gccgccgctt tctcagccag tttcgcttcc
    16441 agggcatcga gggtggatgc gccatcggca gaaaccacgc cgattttatt catgttctgt
    16501 gcctgggatg ccgttaccgg ttccgccgcg aacacgccaa atgacagagt tgaaagagcc
    16561 atagcagcaa caacagtatt gatagttttc ataattaatc tctcgcaggt gatttttgta
    16621 taagtgacgt tgtttcgtcg atgtgatgag tatcacgttt ttttacgaga gataaaatcg
    16681 aagagaattg accgccttgt tcaaataaat tgattgatat ctaacatact gataaaccat
    16741 ttatttgttg tacttatgga cttgttttat gccgcgtggc agtcacagca gcgttaaagg
    16801 cacggaatgc cacgcaaaat acaaattata ctcgctatgc atctcgccag ttgtagttaa
    16861 cgtaactggc tgtctttcga acctctgcga ttatatcctg tatacgctgg tttttgtaaa
    16921 tctttctcct gctgacaatg aatacataag cgcacgccag gaatggcttc ccgacgggct
    16981 tgcgggatgg gggcaccgca ctcttcacat tcatacaggc tttcgccacg cggaatttca
    17041 ccccgagcgc gggcaatcgc atcttcaatt gtactgttga tctgttcgtt gacggcgtca
    17101 tcgttagccc aagcggatgc catatcgtcc tccccatatc aatgcttgta cagttaagtg
    17161 tagctaatat agggacgaac ctgggcagtt caagcgtcag atctccgacc attcccgcag
    17221 cagattatga taaagattga gcagcgacag gatctcttca ttttcaccat agcggctttt
    17281 cagcgactga atatttttgt ccagttcaaa cagcatggcg cgctttttat catcgcggat
    17341 catcgactgg atccacataa atgatgccac tcgtacgccg cgggttacgg gtgtcacgca
    17401 atgcaggctg ctggagggat acaacaccag atcacctgcc gggagtttta cccgatgttg
    17461 tccaaaggtg tcattaacga ccagttcgcc gccgtcgtag ctttgtggat cgcttaaaaa
    17521 cagcgtggca gaaaggtcag tgcgcatcca gccgttttgt ggatggctgc gtactgcgcc
    17581 atccacatgg aaaccatagg tttcattgtt ctgatagcga ttaaacagcg gcgtggaaag
    17641 ggtacgcggc aaggcagcgg caaagaacaa tgcatgttgg ttaaccgcgt tcagcacctc
    17701 attttgcagt gcggcgtata acgcgctgcg ggtgtctact tgctgattgt tcttaacttg
    17761 tgcaccttgt gctccggtgg tgacgcgtcc atccacccat tcggcttgtt ccagttgttc
    17821 gcgaaaatgg gcgacgtcct gtggcgataa cacgccggga atgtggtaca tcatacaagt
    17881 tctccaaaaa gtggggcctg cgccccacat ctgaatcaga aatgcatatt ggctgtgagc
    17941 aagaaggttc ttggctcgcc cgggtgataa cggtagccgc tcttgttgat tgaggcgacg
    18001 taatcggtat caaacaggtt gtaaacgttt agctggaagt cgagattgcg attaacccga
    18061 taccccagtt tggcatctgc gacccagtaa ccttctgtaa atgctggcgt tcccaccgcg
    18121 ccgtctgaac ctttatgtat actgccgata tagcgtgcgc ccgcgccaac agagatatca
    18181 tcggttgcct gatattggct ccataaggtg aaggcgtgct ccggggtata cggcagcgat
    18241 gaggaaccat cctgggcaac atctttgccg tttttgatgg ttgctttttt ctgggtatag
    18301 ccgccaatca cctgccacgc gggagtgata ttccctgcca cggatatctc atagccttcg
    18361 acgcgtttct taccgtattg cgagtaagtc ccgtcatcat tttgctcaac ttcattttcg
    18421 atatcagtac ggaacagcgc ggcggtgagc aacaggcgtt tatccagaac ctgccatttg
    18481 gtgccaatct cgctggtgtt ggctttttgc ggtttaaaat cggtgcggtt ggcactgtta
    18541 ccactgccag actgcgcaag ggcgaagttg ttgccgcccg gaggctgctg ggaaacggca
    18601 tagttaatat agacattgcc gttttccgtc aggtgataca gcgccccggc tttccagttc
    18661 accagattgc ccgacttggc ggtgtcgacg gtggtgactg gagaaccttt tgccacacca
    18721 gctgggcagg tgatggcacc gcgtccgctg ccgccgcagg cggtggcact gtcatattca
    18781 gtatgataat tatccagacg gatcccgccg ttcagctcaa aatcacgggt gatttgcagc
    18841 gtatcgaagg cgtaaattgc gaaggtatcc gtctgaccat tggcgtttgc gccgttgcgc
    18901 gtcaggccgc cgggatgaat tctgctgtca ggatgataaa tatttaccgc cggtaacgtc
    18961 accggattaa cgccatagtt agtctgcgtc tcacgggtaa attccacgcc ggtactgaca
    19021 tcatgaccga tagaaccggt atagaacgtc gaggtcaggt tggtctggtt ggtgagaatt
    19081 ttattactca catctttggt attcgccgtg cgtgactagg tccagctatt cacatcgctg
    19141 gtgggctgag taatattcga cgccccgccc ataatcgccg tcatcaggta atcctgcttt
    19201 acgcgcgacc aacgggtagt attgcgaatg gtggtgttat cgttgatgtc gtgctcaaaa
    19261 cgcatggtgg cggtgtcggt ggtcgaatcg tcgtaatcgg aatccgtgcc gtaaaagtta
    19321 tgagtatcaa cttttccgga atgattcagg gccgccgctc ccgcagatgg ggcagaatag
    19381 cccggcaaac cgatggtcgg aatgccgccg tctggcgtgt tgtgctgagt gacatgcaga
    19441 taattaagat acaaacgatt cgctgtacca aggccaaaag agacagaagg ggcgacgccg
    19501 taacgctcat ttttgacttt gtcgcgtccg gcatcgtggg ttttttcgcc catcacattc
    19561 aggcgcaccg cggtagtatc accaatgacc tgattgacgt ccagcgtgcc gcggcggaac
    19621 caggcgctgc caatactggc ggaggcgtca atgccggaat cattgcgcgg ctgcttgctg
    19681 atcatattga tcgagcctgt cggtgcgctg cgcccgtagt cggtgccgga cggcccttta
    19741 atcacttcga cctgctcggt attgaaggtg tcgcgcgaga cgctgccgat atcgcgaata
    19801 ccatcaatat aaatactgtt agaggtatcg gcaccacgca tataaatggc gtcgccagtg
    19861 gtggagttac cgttctcacc cgcaaaaaac gcacccacgc
//
LOCUS       DADMGC010000074        19787 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.074, whole genome shotgun sequence.
ACCESSION   DADMGC010000074 DADMGC010000000
VERSION     DADMGC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.074"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            10..276
                     /locus_tag="JNZ73_002529"
     CDS             10..276
                     /locus_tag="JNZ73_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8732771.1"
                     /translation="MNNPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVV
                     TLIDDNIREEILRPEWIAGETGMSVRSLYRMFADIRFGSRAIYS"
     gene            251..442
                     /locus_tag="JNZ73_002530"
     CDS             251..442
                     /locus_tag="JNZ73_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY8732772.1"
                     /translation="MVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFS
                     TVFKQRFGMTPGEYRRKFR"
     gene            complement(614..943)
                     /locus_tag="JNZ73_002531"
     CDS             complement(614..943)
                     /locus_tag="JNZ73_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HAY8732773.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(948..1133)
                     /locus_tag="JNZ73_002532"
     CDS             complement(948..1133)
                     /locus_tag="JNZ73_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HAY8732774.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(1130..3769)
                     /locus_tag="JNZ73_002533"
     CDS             complement(1130..3769)
                     /locus_tag="JNZ73_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HAY8732775.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHVGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3977..4966
                     /gene="ldhA"
                     /locus_tag="JNZ73_002534"
     CDS             3977..4966
                     /gene="ldhA"
                     /locus_tag="JNZ73_002534"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY8732776.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            5077..5424
                     /gene="hslJ"
                     /locus_tag="JNZ73_002535"
     CDS             5077..5424
                     /gene="hslJ"
                     /locus_tag="JNZ73_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HAY8732777.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEISFGEKMMISGSMCNR
                     FSGEGKLSNGELTAKGLAMTRMMCANPQLNELDNTISEMLKEGAQVDLTANQLTLATA
                     KQTLTYKLADLMN"
     gene            complement(5421..5687)
                     /locus_tag="JNZ73_002536"
     CDS             complement(5421..5687)
                     /locus_tag="JNZ73_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HAY8732778.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5961..9485
                     /gene="nifJ"
                     /locus_tag="JNZ73_002537"
     CDS             5961..9485
                     /gene="nifJ"
                     /locus_tag="JNZ73_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAY8732779.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAGAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9852..10981
                     /gene="ompN"
                     /locus_tag="JNZ73_002538"
                     /pseudo
     CDS             9852..10981
                     /gene="ompN"
                     /locus_tag="JNZ73_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            11122..11556
                     /gene="uspF"
                     /locus_tag="JNZ73_002539"
     CDS             11122..11556
                     /gene="uspF"
                     /locus_tag="JNZ73_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HAY8732780.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11732..12667
                     /gene="ttcA"
                     /locus_tag="JNZ73_002540"
     CDS             11732..12667
                     /gene="ttcA"
                     /locus_tag="JNZ73_002540"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAY8732781.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(12796..14163)
                     /gene="dbpA"
                     /locus_tag="JNZ73_002541"
     CDS             complement(12796..14163)
                     /gene="dbpA"
                     /locus_tag="JNZ73_002541"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HAY8732782.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTLALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(14641..15624)
                     /gene="zntB"
                     /locus_tag="JNZ73_002542"
     CDS             complement(14641..15624)
                     /gene="zntB"
                     /locus_tag="JNZ73_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HAY8732783.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            15879..17111
                     /gene="dgcM"
                     /locus_tag="JNZ73_002543"
                     /pseudo
     CDS             15879..17111
                     /gene="dgcM"
                     /locus_tag="JNZ73_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(17132..17695)
                     /gene="smrA"
                     /locus_tag="JNZ73_002544"
     CDS             complement(17132..17695)
                     /gene="smrA"
                     /locus_tag="JNZ73_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HAY8732784.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(17949..19403)
                     /locus_tag="JNZ73_002545"
     CDS             complement(17949..19403)
                     /locus_tag="JNZ73_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY8732785.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGILQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(19400..>19787)
                     /locus_tag="JNZ73_002546"
     CDS             complement(19400..>19787)
                     /locus_tag="JNZ73_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY8732786.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         5203 a         5011 c         4665 g         4908 t
ORIGIN      
        1 caggagagca tgaataatcc ggcactttct gaaacagaaa gtgaagctgc gctacaggcg
       61 atggtgtgtc tgcttcgccc agtacttcat cagcgggaat ctgtccaacc tcgccgtgaa
      121 cgtcagtttc aaaaagtggt tacgttgata gacgataata ttcgcgaaga gatattacgc
      181 ccggagtgga tagccggaga gacaggtatg tcagtacgta gtttgtaccg aatgtttgcc
      241 gatataaggt ttggtagtcg cgcaatatat tcgtaaccgt cgtctcgatt tttgtgcaga
      301 tgcgattcgc catgccgcag atgatgaaaa actggcaggc atcggctttc attggggatt
      361 ttctgaccag agtcatttct caacggtatt taagcaacgc tttgggatga cgccaggcga
      421 gtatcgccgt aaattccgct aactaattgg cgttgcatgt actgcaacgc caattagtta
      481 aaacagatat ttgactatgc atttttcagg gcgaaaggca atttgacagg agtttttgaa
      541 tattcacctt gtttcaggaa aatagatcaa tcgctttctc ctgtagcgtt atcccgatat
      601 tgcggttttg ctattaaaac tttcgcaccc atttaccgtt tatcccttgt acatattccc
      661 ccggtttggc ccgagctacg agcttttgcc cggcgagttt cgcgatatca tctacagaca
      721 cattattttg ctttgcccgt tgttggtaac tcgctttacg ttcggcatta atttcggtta
      781 ctaatttctc tgtttcagca tccgttttca gcgcaaccag ataaccataa aatgtttcac
      841 ctacccgccc ctgagttctg gcttcatcca gagtcaatgc cattacattg ctgcttacca
      901 gcccaacaag aaacgcacaa agaagtaatg ttttcttcat catcgcctca gaaaagatcg
      961 ctacgagttt caagcagctc ttcgacatct ttgtctgcct tgatgatgat ctcatgctca
     1021 attttaacgt tcatattgat agtgatcggt tccttaggtg cagcgacttc aatgcgaggc
     1081 gtacagccaa ccagcataaa agatgacgtc aacgcagcca gtaaaatttt cattgttttt
     1141 cctcacactc ttttccttgc ggacagtcat ttcccggcag acgtgcgttc tgctccaacc
     1201 atgcctggag attatcgcca aagcgtaaac tgcgccacag cgtaaaaata ttctcttcat
     1261 gatggtaatt aaggtttacc gtaccgcttt taccatcaac gcgactggta cctgtaatgt
     1321 tggcctgcat ggttaataaa ccgagattat ctaagttaat ttttgtcgat gaccggctaa
     1381 tttccatata gcgcaaccag ttaattgctg aacccgcagt catattgtct ttcaccaccg
     1441 catctgcggt atctttatcc agccgcaagg tcattggccc gctatttgcc agccaaccat
     1501 ctttcactat ccatttttcg ttgtttagcc ataacggcaa tgcaccacta aatgccccgg
     1561 acatggcgaa ttgtttcgga ttgacggcgc taaccagttc gctggatgag agattattca
     1621 gccgcaacag cgccgggtca tgttgcggca tacgtaattg tttcatcagc acgttaccgc
     1681 ctaacacatc gacgctaaca tcagtcaaca gcaagggttc ttcttcagtc cacggataac
     1741 gcccttgcaa atcagccgta atatttttcg ctgtcaccag attaatcact tcggcaattc
     1801 gcaacgtaac ggggccgcga gtccccagat gccaggctcc atcggcaaaa cggaaaggca
     1861 ggacaaaatc gacaccgtta acctgattat ctggcatcca ggcactaccg cctttcaaca
     1921 cgccgtgccc tcccgcgcgg aatccttgtt caggcgcagc agaaaatgca acctgagcat
     1981 acagttcacc atcgcgtaag ttcatcttcc agtcgggtgg caccagcggc tggaatacag
     2041 tcagtgattg ttttggccac caggcgttac cgcgcagacg aataccgtcc cagcgaccat
     2101 ttacccgaac cgggccaatc tcaccaacat gtaaatcgcc tttaaagagg aaataggtcg
     2161 gatcgcgccc atcgacgcta aattttaagg ttgatggcgg taacacactg ccgccagtga
     2221 acagcgtttg cccggcgtcc agtgacagcg cgccgctgaa ggagggatgc tgtgcgtcac
     2281 gtacccagac gacgggcttg tcgagaatta atcgcggctt ttctaccgtc atcgtaccgt
     2341 attgtaactg gtcgaaaccg gtggagagat cggtcagcgt aatcgtgctg tcatgccact
     2401 caccttttcc tgcgacatcc caggtggcat tcatcggtgt aaaactccct tttccccagt
     2461 agcgccactg ccagcggcca gcgtcaggga gaaaatcatt cgccagccca tccatatgca
     2521 gcacgaaatc gcccagttca ttttcatgcg cctgcaaaat ggcctgcaaa cgtccgtcaa
     2581 caccacgttg ggtgactttt acacccgcta aaggccagcg gatttcatcg atgtccagcg
     2641 aatcgatgac tcttcccttt gaacgaagta acgcgcctgg ctcaaaggtc agcgttgggt
     2701 cagacagact tccacttagc tgcgcaggta aacgggcata taaaatgagg tccgcctgtt
     2761 tcgcttcacc ggtcagctgc agaggcagtt gactgttatc catgcttaat tttcctgggc
     2821 caaaattaag caccgcgttg cccttacccg cttgcccctg ggtcagcaca ctcagtcgtc
     2881 cgctgaccag agcgttctca agccctgcct gccagttgtc aactttgaca cccagtcggc
     2941 cactcaaagg aaaacctgca tacagccagc tccagcgacc atcgcttacg gtcaattgtt
     3001 gacgagtaat ttgccacggc aaatcgagca acggatcgcc gttatcccgt gccagcacga
     3061 tcaattgccc gctattttcc tgccagtcca gctcggcatc caccagtgac ggttcctgcg
     3121 gcaagtttaa cgtcgcagta gcatgcccac ttacaggaag tccatccggc acgagcggca
     3181 tagtaaattc ccccaccagt ttaaccggcg gctgattttc aaacgcgacg acatccagtt
     3241 cgctgactgt aagttgttgc cctttcagct ggccttgaaa tttaactttt tcgccctgat
     3301 aacgcagttg ctggatatca gaggttaatg cgagagagag ttttccctgc cattcctgcc
     3361 agggagaaaa aatcagttta tcgatattga tccaggtgtt aggcagcatg gactgccact
     3421 gcgcgagggt ttttggagca gctggcgatt gctccgtctg cggcaatttc gccagacaag
     3481 cagaatcaag ttctaccgtg ccgacgttca ataaccagcg gctgggatgt gaaaggctgg
     3541 cgttggtgat atgcgcaagc tgacaatctc ccaccagata acggagatcg ggaatgatta
     3601 aacctttacg cgtaatgcgt gggctttcat ctaatgcaat acgtgtcccg agcggtagcc
     3661 agatgtccgc cagcgtggga acccacagcc cgagcgtcat cagcagcgtc aacggcacaa
     3721 gaataatcag taataacagc gcgagaacgg ctttatattt acccagcatg ggtagttaat
     3781 atcctgattt cgcgaaaaat taagcattca atacgggtat tgtggcatgt ttaaccgttc
     3841 agttgaaggt tgcgcctaca ctaagcatag ttgttgatga atttttcaat atcgccatgg
     3901 ctttcaatta tatttgaaat tttgtaaaat atttttagca gcttaaatgt gattcaacat
     3961 cactggagaa agtcttatga aactcgccgt ttatagcaca aaacagtacg acaagaagta
     4021 cctgcaacag gtgaacgagt cctttggctt tgagctggaa ttttttgact ttctgctgac
     4081 ggaaaaaacc gctaaaactg ccaatggctg cgaagcggta tgtattttcg taaacgatga
     4141 cggcagccgc ccggtgctgg aagagctgaa aaagcacggc gttaaatata tcgccctgcg
     4201 ctgtgccggt ttcaataacg tcgaccttga tgcggcaaaa gaactggggc tgaaagtagt
     4261 ccgtgttcca gcctatgatc cagaggccgt tgctgaacac gccatcggta tgatgatgac
     4321 gctgaaccgc cgtattcacc gcgcgtatca gcgtacccgt gacgctaact tctctctgga
     4381 aggtctgacc ggctttacta tgtatggcaa aacggcaggc gttatcggta ccggtaaaat
     4441 cggtgtggcg atgctgcgca ttctgaaagg ttttggtatg cgtctgctgg cgttcgatcc
     4501 gtatccaagt gcagcggcgc tggaactcgg tgtggagtat gtcgatctgc caaccctgtt
     4561 ctctgaatca gacgttatct ctctgcactg cccgctgaca ccggaaaact accatctgtt
     4621 gaacgaagcc gccttcgatc agatgaaaaa tggcgtgatg atcgtcaata ccagtcgcgg
     4681 tgcattgatt gattctcagg cggcaattga agcgctgaaa aatcagaaaa ttggttcgtt
     4741 gggtatggac gtctatgaga acgaacgcga tctgttcttt gaagataaat ccaacgacgt
     4801 gatccaggat gacgtattcc gtcgcctgtc tgcctgccac aacgtgctgt ttaccgggca
     4861 ccaggcattc ctgacagcag aagctctgac cagtatttct cagactacgc tgcaaaactt
     4921 aagcaatctg gaaaaaggtg aaacctgccc gaacgaactg gtttaatctt gccgctcccc
     4981 tgcaatccag gggagctgat tcagataatc cccaatgacc tttcattctc tattcttaaa
     5041 atagtcctga gtcagaaact gtaattgaga accacaatga agaaagtagc cgcgtttgtt
     5101 gcgctaagcc tgctgatggc gggatgtgta agtaatgaca aaattgctgt tacgccagaa
     5161 atcagctttg gtgaaaaaat gatgatttcc ggcagcatgt gtaaccgctt tagcggtgaa
     5221 ggcaaactgt ctaatggtga actgacagcc aaagggctgg caatgacccg tatgatgtgc
     5281 gctaacccgc agcttaatga actcgataac accattagcg aaatgctgaa agaaggtgca
     5341 caagtggatc tgaccgcgaa ccagttaacg ctggcgaccg cgaaacagac attaacttat
     5401 aagctggcgg atttaatgaa ttaatagctg ccacagctcc cggcggcaag tgactgttcg
     5461 ctacagcgtt tgccgttggg taatgcacac atcccaatcg ccgtaccatc cagttgacgg
     5521 gcaacagaaa gcgaaccgcc gatcattgca caatttgctt ctccactact ggacatcgac
     5581 gcttttaaac ttggcgctac gtgcgccgca gtcgcctgct gaacaggctc actactacac
     5641 gccgacaaca ataaagcggc acaccctacc caaaacgctg ctcgcatctc ttcttcctct
     5701 gatcttcaag ccaaacgaca ccgccataaa taataggcag cacagagggc ggcgtcgaga
     5761 gctgtcctgc gcgttgcccc gcatttttac ttttttatgg ctattttttt gccctctgtt
     5821 tgatcaaaac attcattact ctgatgtggg ggacacaaaa gcgaaaatgc agaagaaagc
     5881 catttgctaa aattgaagga ttactactgg gcgcgcagca atttcgtgcg cccctcattt
     5941 gcgcaatgta agggtgtcat atgattacta ttgacggtaa tggcgcggtt gcttcggtgg
     6001 cgtttcgcac cagtgaagtt atcgccatct accctattac ccccagttcc acgatggcag
     6061 aacaggctga tgcctgggcc ggaaacggct tgaagaacgt ttggggagac acaccacgcg
     6121 tggttgaaat gcagtcggaa gcgggtgcta tcgctaccgt gcatggcgct ttgcagacgg
     6181 gtgccctttc aacatcgttt acgtcatcgc agggtttgct gctgatgatc ccgacgctgt
     6241 acaaactggc aggcgaactg acgccatttg tcctgcatgt agcggcacgt accgttgcca
     6301 cacatgcact ttctattttt ggcgatcatt ccgacgttat ggcggtgcgc cagacgggtt
     6361 gcgcgatgtt gtgtgcggct aacgtccagg aagcgcaaga ctttgctctc atttcacaca
     6421 tcgcaacgct gaagagccgc gtgccattta ttcatttctt tgatggtttt cgcacgtccc
     6481 acgaaatcaa taaaattgtc ccgctggccg atgacactat tcttgatctc atgccgcagg
     6541 ccgaaattga tgctcatcgc gcccgggcgc tcaacccgga acatccggtg atccgcggta
     6601 cgtccgccaa tcctgacact tatttccagt ctcgcgaagc caccaacccc tggtacaacg
     6661 cggtctatga ccatgttgaa caggcgatga atgatttcgc tgccgcgaca ggtcgtcagt
     6721 atcagccgtt tgagtattac gggcatccgc aagcggaacg ggtgattatc ctgatgggct
     6781 ctgccattgg cacctgtgaa gaagtggttg atgaattgct aacccgtggc gaaaaagttg
     6841 gcgtgctgaa agttcgtctg taccgtccct tctccgccaa acacttgctg caagctctgc
     6901 cgggatccgt acgcagcgtg gcggtactgg acagaaccaa agaacccggt gcccaggcag
     6961 aaccgctcta tctggatgtg atgaccgcac tggcaggagc ctttaataat ggtgagcgcg
     7021 aaaccctgcc ccgtgtcatt ggtgggcgct atggtctttc atccaaagaa tttggcccgg
     7081 actgtgtact ggcggtattt gccgagctca acgcggctaa acctaaagca cgctttacgg
     7141 ttggtattta cgatgatgtg accaatctgt cactgccgtt gccggaaaac actctgccaa
     7201 actcggcgaa acttgaagcc ttgttttatg gccttggtag tgatggcagc gtttccgcaa
     7261 ccaaaaacaa tatcaagatt atcggtaatt ccacgccgtg gtacgcacag ggctattttg
     7321 tttacgactc caaaaaagcg ggcggcctga cggtttctca ccttcgcgtg agcgaacagc
     7381 cgattcgttc cgcttatctc atttcccagg ctgattttgt tggctgccac cagttgcagt
     7441 ttatcgataa atatcagatg gctgagcgtt taaaacctag cggcattttc ctgcttaata
     7501 cgccgtacag cgcagatgaa gtgtggtcgc gcttgccgca ggaagttcag gccgtgttaa
     7561 accagaaaaa agcgcgcttc tatgtgatta acgcggcgaa aatcgcccgc gaatgtggcc
     7621 tggcggcccg tattaatacc gtcatgcaga tggctttttt ccatctgacg caaattctgc
     7681 ctggcgatag cgccctcgca gaattgcagg gtgcgattgc caaaagttac agtagcaaag
     7741 gtcaggatct ggtggagcgc aactggcagg ctctggcgct ggcgcgtgaa tccgtagaag
     7801 aagttccgtt gcaaccggta aatccgcaca gcgccaatcg accgccagtg gtttccgatg
     7861 ccgcccctga tttcgtgaaa accgtaaccg cggcgatgct cgccgggctt ggtgatgccc
     7921 tccccgtttc ggcgctgccg ccagacggca cctggccgat gggcaccact cgctgggaaa
     7981 aacgcaatat cgccgaagag atccccatct ggaaagagga actctgtacc caatgtaacc
     8041 actgcgtcgc cgcttgccca cactcagcta ttcgcgcaaa agtggtaccg cctgaagcga
     8101 tggaaaacgc ccctgccagc ctgcattcgc tagatgtgaa atcgcgtgat atgcgcgggc
     8161 agaaatatgt cttgcaggtg gcaccggaag attgcaccgg ttgtaacctg tgcgtcgaag
     8221 tttgcccggc gaaagaccgt cagaatccag agattaaagc catcaatatg atgtctcgcc
     8281 aggaacatgt cgaagaagag aaaatcaatt acgatttctt cctcaacctg ccagaaatcg
     8341 accgtagcaa actggaacgt attgatattc gtacatcgca gctgattaca ccgctgtttg
     8401 aatattcagg tgcttgcccc ggttgtggcg agacgccgta tattaaattg ctgactcagc
     8461 tctatggtga ccggatgttg atcgctaacg ccaccggctg ttcttcaatt tatggcggta
     8521 acctgccctc tacaccgtat accaccgatg ccaacggtcg tgggccggca tgggcgaact
     8581 ctctatttga agataatgcc gaatttggcc ttggtttccg cctgacggtc gatcaacacc
     8641 gtgtccgcgt gctgcgtctg ctggatcaat ttgccgataa aatcccggcg gaattactga
     8701 cggcgttgaa atcagacgct acgccagagg ttcgtcgaga acaggtcgca gctttacgcc
     8761 agcaactcaa cgatgttgcc gaagcacatg aactgctgcg tgacgcagat gcactggtgg
     8821 aaaaatcaat ctggctgatt ggtggcgacg gctgggctta cgatatcggc tttggcggtc
     8881 tggatcatgt attgagtttg acggaaaacg tcaacattct ggtgctggat acgcaatgct
     8941 attccaacac cggtggtcag gcgtcgaaag cgacaccgct gggtgcagta actaagttcg
     9001 gcgagcacgg caaacgtaaa gcgcgtaaag atcttggcgt cagtatgatg atgtacggtc
     9061 atgtttatgt ggcgcagatt tctctcggcg cgcagctgaa ccagacggtg aaagcgattc
     9121 aggaagcgga agcgtatccg ggaccatcgc tgatcattgc ttatagcccg tgtgaagagc
     9181 atggttacga tctggctctc agccacgacc agatgcgcca actcactgct accggcttct
     9241 ggccgctata tcgctttgat ccgcgtcgtg ccgatgaagg caaactgccg ctggcgctgg
     9301 attcacgccc gccgtcagaa gcactggaag aaacgttact tcacgagcag cgtttccgtc
     9361 gtctgaattc gcagcagcca gaagtggcag aacagttatg gaaagatgct gcagctgatt
     9421 tgcaaaaacg ctatgacttc ctagcacaaa tggccggaaa agcggaaaaa agcaacaccg
     9481 attaaatgct ctggataagg attatccaat tctaaaaaaa aagcccggac gactgttcgg
     9541 gcttgtcttt ttatatgttg gaaaatcagt ggcaatgcaa tggcccaaca gaaaataaaa
     9601 tctgtgacaa taaaggcata taacccgcgc agaataacgt atagaaaatt aaaagtataa
     9661 tttttatttt gtatgtatta tcttcgatac attcaccaga cttattatca tttcagagga
     9721 attatttgat taaggtttta cttaaggcgt aacaaatgat atttatcggc aaactgaact
     9781 tctcctttag gatgttttca ctccccgaaa tggggataaa aggcaaataa aataactaaa
     9841 ggatttattc aatgaaaaga aaagtactgg cacttttaat ccctgccctg ctcgccgcag
     9901 gtgctgcaca tgcagccgaa gtttataata aagacggcaa caaattagat ctgtatggca
     9961 aagttgatgg cctgcattat ttttctgata attcagcgaa agatggcgac cagagctatg
    10021 cgcgtctggg ttttaaaggc gaaacccaaa ttaacgatca acttactggc tacggtcaat
    10081 gggaatacaa tattcaggca aacaacactg aatcttcaaa aaaccagtca tggacccgtc
    10141 tggcatttgc cggactgaaa tttgcagatt acggttcttt cgattacgga cgtaattatg
    10201 gcgtaatgta cgacatcgaa ggctggaccg atatgctgcc taaatttggc ggtgactctt
    10261 ataccaatgc agacaacttt atgactggtc gagccaatgg cgtcgcgact tatcgtaata
    10321 ctgatttctt cggtctggta aatggtctga acttcgcggt gcagtatcaa ggtaacaacg
    10381 aaggagccag taatggtcag gaaggcacca ataacgaacg cgatgttcgt catgaaaacg
    10441 gtgacggctg gggtctttcc acaacatatg atttaggcat gggctttagc gctggtgcgg
    10501 catacacctc ttctgaccgc accaatgacc aggttaacca tactgcggcg ggtggtgata
    10561 aagcagacgc gtggactgct gggctaaaat acgatgctaa caatatttac ctagcaacca
    10621 tgtattcaga aacgcgtaat atgaccccgt ttggcgacag cgattatgca gttgcgaaca
    10681 aaacccagaa ctttgaagtt actagtacca gtttgatttt ggtctgcgtc cggcagtctc
    10741 tttcctgatg tctaaaggcc gtgaccttca cgctgctggt ggtgcagaca acccggcagg
    10801 tgttgatgat aaagatctgg ttaaatacgc cgatgttggc gcgacttact atttcaataa
    10861 aaacatgtcc acctatgttg actataaaat caacctgttg gatgaagatg acagtttcta
    10921 cgctgccaac ggcatctcta ccgatgatat tgtcgcttta ggtctggttt atcagttcta
    10981 aatcctcctg cccgctgtta tggcgggctt tttctgctta ttcttcctcc tttgatctaa
    11041 attaaaaatg tgaactccgt cattacacaa aaagtgtcat ctggcgttac actttatgcg
    11101 gatactaaaa caggaggttt tatgaacaga acgattcttg tccctatcga tatttcagat
    11161 tcagaattaa ctcaacgcgt gattagccac gttgaggcag aggcaaagat tgatgatgca
    11221 gaggttcatt tcctgacggt aataccttca ctgccctact atgcctctct gggtttagcg
    11281 tattccgcag aattaccggc aatggatgac ctgaaagcgg aagccaaatc gcaactggaa
    11341 gagatcatta aaaaatttaa actgccaacc gacagagtgc atgtccatgt tgaggaaggc
    11401 tcgcccaaag accgcattct ggaattggcg aagaagatcc ccgctcatat gatcatcatt
    11461 gcttcccatc gaccggatat caccacttat ctgctcggtt ccaacgccgc agctgtagtg
    11521 cgtcacgcag agtgctccgt gctggttgtg cgctgacact aacgcccgca cattgctgcg
    11581 ggctttttga ttcatttcgc aaatgtgctg acattttccc ctctaatccg taccatacac
    11641 gccacagttt ttatatcaga tttcttatgc tgagcgttcc ggcatgacgc atactcttct
    11701 gatgccatat aacgaattga gtcgctttta aatgtcgcaa aatcaagaaa ttagtaagaa
    11761 agaacaatac aacctgaaca aattacaaaa acgtctgcgt cgtaacgtgg gcgaagccat
    11821 tgctgacttc aatatgattg aagaaggcga tcgcatcatg gtttgcctct ccgggggtaa
    11881 agacagctat accatgctgg agattctgcg caatttgcag caaagcgcgc caatcaattt
    11941 ttcgctggtg gctgttaacc tcgatcaaaa gcaaccgggc ttcccggaac acgttctgcc
    12001 cgagtatctt gaaaagctgg gcgttgagta caagattgtt gaagagaaca cttacggtat
    12061 cgtgaaagag aagattccag agggcaaaac cacttgctca ctgtgttctc ggctacgtcg
    12121 cggtatcctt tatcgcaccg caacggaact ggggacgacg aagatcgcgt tgggtcacca
    12181 tcgtgacgat atcctgcaaa cgttgttctt aaatatgttc tacggcggca agatgaaagg
    12241 tatgcctccg aaactgatga gcgatgatgg caaacatatc gttattcgtc cactggccta
    12301 ctgccgcgag aaagatattc agcgatttgc cgatgcaaaa gcgttcccga ttattccgtg
    12361 caacctgtgc ggttcacagc ctaacctgca acgtcaggtg attgctgaca tgttgcgtga
    12421 ctgggataaa cgttatccag ggcgtatcga gacgatgttc agcgcgatgc agaatgtggt
    12481 gccgtcgcat ctgtgcgata ccaacctgtt cgatttcaaa ggcattaccc acggttctga
    12541 agtggttaac gggggtgatc tggcgtttga tcgcgaagag atcccactac aaccggcggg
    12601 ctggcagcca gaagaagatg aaaatcagtt ggatgagtta cggctgaatg tggttgaagt
    12661 gaaataacca ggatagcgcc cgatgcgcaa gcgtatcggg ctactcttat ggaggccgga
    12721 taagacgcgg ccagcgtcgc atccggcaat cccgaataag atgtttactc ttgcacccgg
    12781 caattcaaca tttcattatt ttaataaccg cacccggcac gtttttcctt taatcttccc
    12841 gccctgtaac tgtttccatg ctttatgagc aacagcctga cggaccgcga catagacatg
    12901 cgccggatgc acggcgattt tgccaatatc tgcgccatca agcccgatat ctcctgtcag
    12961 tgcacctaat acatcacccg ggcgcatttt ggcttttttc ccgccatcga tacacaacgt
    13021 tgccatttct gcttccagcg tcacaatgga actattagct ggcggcgttt gccagttaag
    13081 ttttatctgc aacatgtcag aaatgatatt ggcccgctgt gcttcttccg gagcacagaa
    13141 actgatcgcc agaccgctat ttcctgcacg agctgtacga ccgatgcgat gtacatgaac
    13201 ttcagggtcc cacgccagct caaagttcac caccagctca agcgatttaa tatccagacc
    13261 acgcgcagca acatcagtcg caaccagtac acgggcgcta ccgttagcaa aacgtaccag
    13321 ggtctgatcg cgttgctcca gatcgccgtg taacgacaat gcactttgct ctacttcatt
    13381 cagcgcgtcg cagacagcct ggcaatcttt tttggtattg caaaacacca cacaagagga
    13441 tggctgatgc aagcttaata accgttgcaa cagaggaatt ttgcctttac tggatgtctc
    13501 ataaaattgt tgttcaatgg gtggcaaagc atctgttgag tcaatttcaa tcgccaaagg
    13561 atcgcgttgc actcgtccgc tgattgcggc gatggcttcc ggccaggttg ccgaaaacag
    13621 aagcgtctgt cgagatgcag gcgcaaaacg gatgacatca tcaatggcat cgctaaatcc
    13681 catatccagc atgcggtcgg cctcatccat caccaaagta ttcagcgcat ccagtgatac
    13741 cgtgcctttt tgcaagtgat ccagcaaacg acccggcgtt gccacgataa tatgcggcgc
    13801 atgttgcaac gaatcacgct gcataccgaa cggttgacca ccgcacaacg tcaaaatttt
    13861 ggtatttggc agaaaacgcg ccagccgacg caattcacct gccacctgat ccgccagttc
    13921 acgcgtagga cacagcacta aagccagggt ttgaaatagc gacgcatcaa tttgctgtaa
    13981 caagccgagg ccaaaagccg ccgttttgcc gctgccggtt ttcgcctgca cgcgaacatc
    14041 ttttcctgca aggatcgccg gaagcgcggc ggcctgcacc ggcgtcatgg ttaaataacc
    14101 caactcatta aggttcgtga gttgggcggg aggcaaaaca ttcagggtag aaaaagcggt
    14161 cacaatctat tctcgtggtc atcgacgcaa agttagcagg cgcgtatcct cgcagatcta
    14221 cgctcacgat gcgacaattt aatcggttct tcatcgggtg gtgggtcagg catgggttgc
    14281 gggcgaggga tcggatcggg cactggaaca ggatcgccag gaatcggttc agggacagga
    14341 atttgcaaat aaataagtgt cgtcatattt ccctccggtc attgggtgga ctcttaaagg
    14401 gtagacgctg ataaataaca ggcaaaaaaa agccgactca tcaaagtcgg cgtcgtacga
    14461 atcaattgtg ctatgcagta attcaaaaaa ggaagtaaga caatatggag cgcaacgccc
    14521 atcgcttgac gttgcattca cctgcaaggg agatattgcc ctgaatgggt agagagttta
    14581 ttgacttcgc tcaaactttg cggcgttttt gtatacagac agccgaaaaa attgcttttg
    14641 ttacaaccat ttactacggt gcaaccataa agcaacacca ccaataagaa caactaacag
    14701 aatacaaaaa attgaaaatc cgaattgcca cccgccgcca gggatcccac caaggttgac
    14761 gccaaataac ccggtcagaa aggtactggg taaaaagacc attgccatca acgacattgt
    14821 ataggtacga cgagctaaat tttcctgcat cacctgagcg atttcatccg ccatcacgcc
    14881 agtccgtgct atacaggcgt cgatttcgtc aaggccgcgc ccaaggcgat cggcaatatc
    14941 ctgcatccga cggcgttggt catcgctcat ccacggcaaa cgttcactgg caagacgagc
    15001 ataaacatca cgttgcggtg ccatatagcg acgcatcaca attaattgtt tgcgcagcag
    15061 agccaggaat ccacgcggtg gaatttgctg atcaaggaga ttatcttcaa ggtcgataat
    15121 tttatcgtgc agctgctcga taaattcact ggaatgatcg gtcaacgcat cgcacacatc
    15181 caccagccat cccccgcaat cggtcggacc cgtgccctct tccagatcgc tcaccacatc
    15241 gtccagcgcc agcactttgc gttgtcgggt cgaaacaatt aaccgcccgt ccatatatac
    15301 acgcatggcg accagttgat cggggcgttc atcggtgctg ccgtttatac agcgcaatgt
    15361 aatcagcgtg ccttcaccga aacggctgac tcggggccgt gtgctctcgc ccgccagcgc
    15421 atcacgtacg ttattgggaa gcagcggtgt tgtcgccagc cattgggcgc tatcatggtg
    15481 tacataatta aggtggagcc agcagggatg cgcttcatca atcacatctg tattttccag
    15541 cggtttaacg ccgcctctac catccagcat ccaggcaaat actgcatccg ggacattaac
    15601 gtccgatccc ttaatcgctt ccacagtgcc tccatcatca acgcattatt ttgtagtcta
    15661 gccttctggc cctgttacgc aacatctcat caccccatta ccctgaaatg attaataaaa
    15721 ttctgtctaa attgaataca aaaagcaaaa tgcttttccg tatacaaacc gtgtgaagtg
    15781 ttaaatagcg tctatcatta tcagaattat ctgatcatat gacgtggctt ttttgcgatc
    15841 ggatagcaac aaaaattgat aaaaataacg ggatctcaat gattacgcac aacttcaata
    15901 ccctggactt actcaccagt cctgtctgga ttgtttcgcc ctttgaggaa cagttaattt
    15961 atgccaatag cgcggcgcga ctgttgatgc aagacctcac gtttagtcag ctacgaaccg
    16021 gaccctattc cgtctcctca caaaaagaac tgccgaaata cctctccgat ctgcaaaacc
    16081 aacacgatat tatcgaaatc ctcactgttc agcgtaaaga agaggaaaca gcattaagct
    16141 gtcggcttgt tttgcgagag ctgacagaaa cagaaccggt gattattttc gaaggtatcg
    16201 aagcgccggc aacgctgggt ttaaaagcca gtcgctcggc aaattatcag cgcaaaaaac
    16261 aaggttttta tgcgcgcttt tttctgacta actctgcacc aatgttgttg attgacccgt
    16321 cacgagatgg acaaatcgtc gatgctaacc tcgccgcgct caatttctat ggttataacc
    16381 atgaaacgat gtgccagaaa catacctagg aaataaatat gcttgggcgt cgcgtcatgc
    16441 ctatcatgca tgaaatttcg catttacccg gcggtcataa ccctttgaat tttgttcata
    16501 aactggcgga tggttctact cgtcatgtgc agacctatgc cggaccgatt gaaatttatg
    16561 gcgacaagct catgttatgt attgtgcatg atattactga gcaaaaacgg ctggaggagc
    16621 agctggaaca tgctgctcac catgacgcga tgaccggatt actgaatcgg cgacagtttt
    16681 atcacattac ggaaccaggc caaatgcagc atctcgccat cgctcaggat tacagcttgt
    16741 tgctcatcga caccgatcgt tttaaacaca ttaacgatct ctatgggcat tctaaaggtg
    16801 atgaggtgtt atgcgccctc gcccgccccc tcgaaagttg cgctcgcaaa ggcgatttgg
    16861 tgtttcgttg gggaggcgaa gagtttgtct tattgctacc aagaacccca ctggataccg
    16921 cgctttcgct ggctgaaact atccgcgtaa gcgtggcaaa agtgagtatt tcgggcttac
    16981 cacgctttac cgtcagcatt ggtgtggcgc atcacgaagg aaatgaaagc atcgatgaac
    17041 tgtttaaacg cgttgatgat gctttgtatc gggcgaaaaa tgatggacgc aaccgcgtgc
    17101 tggcggcata agccgcggat gcgtctcgag atcaacgact gcgcttagcg tggcgctccc
    17161 agttttcttg cttcgcctgc gccgttttac gtagtgcgac gtaacacgcc ccgctgccgc
    17221 catgatgcgg tagcgcggtg caatatgcct gaacatcatc aaattcggtc agccagcgcg
    17281 ccacatagct gcggacaata ttggcatgtg atttatcttc ccgcccttta ccatgaataa
    17341 tcagcacatt acgcaaacca tccgccaggg cttgttgaat gaaactgaac accattttgc
    17401 ggcactcttc caccggctgg cgcaaaagat tcaggctcgc ctgttgcgga tatttaccac
    17461 tgcgcagctt atccagcacc ccatgctgca acccttcctg ccgaaactcc agcggctgac
    17521 ttagcgggat gatgtcgaga aatccggtag tgaggaaatt atcaagctgc agcgtgtcga
    17581 tacgctgcgg cgcacgttgg ttacgcgttg gatgccagtg gacatctgta gcacgtttca
    17641 gcggctggac atcttccatg gcgtcaagaa acagcgattt gtcgtcaagg ttcatgtaac
    17701 ctcctatgca ccttcacacg tcgtgattca gacgggttaa ttgttcatac ttactgcatc
    17761 gttcccttca cagattgaaa caatactcta cttcatacct gtgagtatct gcgcaggcgg
    17821 ggcattttat actagcctgt tagcagtatc gaacagttta gcccgagttt aattcagcac
    17881 aggctccgat caggatgatt tattcatagc aggaaccaac aaaaatgcga taaataacag
    17941 accactgatt agcagaaagg taccgaaaat gccatagttt gcgcctccct cagcatcaat
    18001 tgactcaata atttggttga agccagtatt tccgatgtct ggtcacttct actgcgatta
    18061 aaaaataaag cacttctgga aatgaagtca ttcaccgtat ggggaacaga aaccattttt
    18121 tcacccattc tcgaaaaatg aaaattagtt cgattattga gaatagagcc acacagggca
    18181 ataccaatcg cgcccccaag attacgtgtt aaattaaata caccagaggc taacttcagc
    18241 cgttcttttg gaattccccc cagcgttaat gttacgatgg gggccatcgc aaactgctgc
    18301 aaaatccctc taattgcctg tggaaacagt aattcctgcc atccccattc gtgggttata
    18361 ggggtaaaaa gatacattga aaacacaaat ccgcccagcc ccgccatcaa cagccatcgc
    18421 agatttattt ttttacttaa ccagaagtaa aagggaacag aaaataactg gaaaatacct
    18481 gttgtgcaaa cggcaaaacc tatctcttcg gcatttaatc cgcgaatctg ccccaaaaac
    18541 acaggtatta aatatacagt agagaatatc ccaacccctc cagaaaaaga aaaaaaacat
    18601 cccagagtga aatttttatc cttaaaagca tgaagatcca ttatcggatt ggatattgta
    18661 agtgtacgcg cagcaaataa aataaaactc accaatgcca gaactgatgt cagcaaaata
    18721 gtattatcat ccagccatcc ccagcgcgct ccttcttcaa gggtatattc cagacatccc
    18781 agagtcatgg ccaacaggat aatactcgga taatcagcaa ctttcagtaa agagagatca
    18841 ggtttatcaa aattaaccag aaaaggaata ctcaaaacta aatatatgcc aggtaatata
    18901 ttgatataaa atagccatcg ccagtcgagg ttatcagtga gccagccccc cagagttggc
    18961 cctagcgtcg gtgacagtga ggccagcgca ctgactaccg cagcagccaa ccccaactct
    19021 ttaccctggt aataaataaa cgccgtcgtg aaaactaaag ggatcatgga tgcgccagca
    19081 gctccctgta aagcacgaaa caaaatcatg atctgaatat tccatgcaag tccacacgcg
    19141 atactcatta aagtaaatat tcccgcggat agagtaaaaa gccaacgagt ggaaaatacc
    19201 cgtgacagcc acccagagag cggaataaca ataatctcag caattaaata gctggtctga
    19261 agccaggcca tttcgtcttt acctgcggac agaccaccac ctatttcatt tagggatgac
    19321 gatacaattt gaatatcaag ctgagcaata aataatccca tacacatact ggcgaaagca
    19381 atatgtttac gcatagacat catctctcat caacactgac ggtgacggac agtcccgggc
    19441 gaagaagttg ctttaattcc tttgcgtcgt caaatgcaat ccgtaccggg acacgctgaa
    19501 caattttggt gaaattgcca gtggcatttt ctatcggaat caagctgaaa ctggctcctg
    19561 tcgctgggga aaggctttca atgtgaccgt gaaacacttc ccctttgaga atatcagcac
    19621 ggatctcagc cttcattcct ggcttcattc ccgctatttg gttttcttta taatttgcat
    19681 caacccacaa ctcactgact ggtactagtg aaactaacga tgtgccgcct tccacccaac
    19741 tgcctgtatg ggcgctgcgg tttgcgacaa tgccatcaat cggcgca
//
LOCUS       DADMGC010000075        19780 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.075, whole genome shotgun sequence.
ACCESSION   DADMGC010000075 DADMGC010000000
VERSION     DADMGC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.075"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(47..139)
                     /locus_tag="JNZ73_002547"
     tRNA            complement(47..139)
                     /locus_tag="JNZ73_002547"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(103..105),aa:Ser,seq:gct)
     gene            complement(455..640)
                     /gene="csrA"
                     /locus_tag="JNZ73_002548"
     CDS             complement(455..640)
                     /gene="csrA"
                     /locus_tag="JNZ73_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAY8732787.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(875..3505)
                     /gene="alaS"
                     /locus_tag="JNZ73_002549"
     CDS             complement(875..3505)
                     /gene="alaS"
                     /locus_tag="JNZ73_002549"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAY8732788.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3633..4133)
                     /gene="recX"
                     /locus_tag="JNZ73_002550"
     CDS             complement(3633..4133)
                     /gene="recX"
                     /locus_tag="JNZ73_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAY8732789.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4376..5437)
                     /gene="recA"
                     /locus_tag="JNZ73_002551"
     CDS             complement(4376..5437)
                     /gene="recA"
                     /locus_tag="JNZ73_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAY8732790.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5517..6014)
                     /gene="pncC"
                     /locus_tag="JNZ73_002552"
     CDS             complement(5517..6014)
                     /gene="pncC"
                     /locus_tag="JNZ73_002552"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HAY8732791.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYVLQTLWQQ
                     FLQNT"
     gene            complement(6159..7244)
                     /gene="mltB"
                     /locus_tag="JNZ73_002553"
     CDS             complement(6159..7244)
                     /gene="mltB"
                     /locus_tag="JNZ73_002553"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HAY8732792.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7500..8063
                     /gene="srlA"
                     /locus_tag="JNZ73_002554"
                     /gene_synonym="gutA"
     CDS             7500..8063
                     /gene="srlA"
                     /locus_tag="JNZ73_002554"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAY8732793.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8060..9019
                     /gene="srlE"
                     /locus_tag="JNZ73_002555"
                     /gene_synonym="gutE"
     CDS             8060..9019
                     /gene="srlE"
                     /locus_tag="JNZ73_002555"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAY8732794.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9030..9401
                     /gene="srlB"
                     /locus_tag="JNZ73_002556"
                     /gene_synonym="gutB"
     CDS             9030..9401
                     /gene="srlB"
                     /locus_tag="JNZ73_002556"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAY8732795.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9405..10184
                     /gene="srlD"
                     /locus_tag="JNZ73_002557"
     CDS             9405..10184
                     /gene="srlD"
                     /locus_tag="JNZ73_002557"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HAY8732796.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10289..10648
                     /gene="gutM"
                     /locus_tag="JNZ73_002558"
     CDS             10289..10648
                     /gene="gutM"
                     /locus_tag="JNZ73_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HAY8732797.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGHFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10715..11488
                     /gene="srlR"
                     /locus_tag="JNZ73_002559"
                     /gene_synonym="gutR"
     CDS             10715..11488
                     /gene="srlR"
                     /locus_tag="JNZ73_002559"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HAY8732798.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11481..12446
                     /gene="gutQ"
                     /locus_tag="JNZ73_002560"
     CDS             11481..12446
                     /gene="gutQ"
                     /locus_tag="JNZ73_002560"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HAY8732799.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSSTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12443..13957)
                     /gene="norR"
                     /locus_tag="JNZ73_002561"
     CDS             complement(12443..13957)
                     /gene="norR"
                     /locus_tag="JNZ73_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HAY8732800.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGNDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14144..15583
                     /gene="norV"
                     /locus_tag="JNZ73_002562"
     CDS             14144..15583
                     /gene="norV"
                     /locus_tag="JNZ73_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HAY8732801.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15580..16713
                     /gene="norW"
                     /locus_tag="JNZ73_002563"
     CDS             15580..16713
                     /gene="norW"
                     /locus_tag="JNZ73_002563"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HAY8732802.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16841..19093)
                     /gene="hypF"
                     /locus_tag="JNZ73_002564"
     CDS             complement(16841..19093)
                     /gene="hypF"
                     /locus_tag="JNZ73_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAY8732803.1"
                     /translation="MAKNTFCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19246..19773)
                     /gene="hydN"
                     /locus_tag="JNZ73_002565"
     CDS             complement(19246..19773)
                     /gene="hydN"
                     /locus_tag="JNZ73_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HAY8732804.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISCDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
BASE COUNT         4651 a         5511 c         5041 g         4577 t
ORIGIN      
        1 tcggttcgta gccgagtact ctatccagct gagctacgga tgcaaatggc ggtgaggcgg
       61 ggattcgaac cccggatgca gcttttgacc gcatactccc ttagcagggg agcgccttca
      121 gcctctcggc cacctcacca cacgcctctt acgagtgctt cgaagaactt gtttatcgct
      181 catcgtcgct gcgtggcgca catattactt tctgagactt ataagtcaaa caatttttcc
      241 cacactttta tcgtttgcac acttcacgct caattagtct gtaaaaacgt caaaaagagt
      301 gttttatcta cagaaaaacg gaggtctggc agatagcagt aatgcaaaaa aatggagact
      361 taagttgaat gaacgggagt aaagcgaaaa gactatagag tgaaggagaa attttgaggg
      421 tgcgtctcac cgataaagat gagacgcgga aagattagta actggactgc tgggattttt
      481 cagcctggat acgctggtag atctcttcac ggtgaacaga aacttccttc ggggcattta
      541 cgccaatacg tacctggttg ccctttaccc ctaaaactgt cacggtgacc tcatccccaa
      601 tcatgagggt ctcaccaact cgacgagtca gaatcagcat tctttgctcc ttgaaagatt
      661 aaaagagtcg ggtctctctg tatcccggca ttatccatca tataacgcca aaaagtaagc
      721 aacgacaaac acattacatg taagcagtca tggcattaca ttctgttaaa cctaagttta
      781 gccgatatac acaacttcaa cctgacttta tcgttgtcga tagcgttaat gcgaatgccg
      841 tgaagcgagt ccacggcatt gcctgacgct tatattattg caatttcgcg ctgacccagc
      901 ctttcacact ggctaacgct gcaggtaagg ccgcagcatc cgtaccaccg gcttgcgcca
      961 tgtcaggacg tccaccaccc ttgccgccca cctgctgagc gaccatacca atcagttccc
     1021 ctgctttcac acgatctgtg acgtccttag atacgcctgc aatcagagaa accttacctt
     1081 cggctaccgt tgccagcacg ataattgtcg accccagctg attttttaaa tcgtcaacca
     1141 tggtacgcaa cattttcggc tcaacaccgc taagctcgct aaccaacagc ttaacaccat
     1201 taacatcaat tgccttactg gaaagatttg cgctctcctg tgcggcagct tgttctttaa
     1261 gctgttgtag ttctttttct aactgacgcg tacgttccag tactgagcgc actttatccg
     1321 ccagattatt gctatcgcct ttcagcagat gcgcgacttc gcttaagcga tcgctgtctg
     1381 catgaacggt ggtgatagcg ccttctccgg ttaccgcttc gatacgacga acgcctgcag
     1441 cagtacccga ttcagagatg atgcggaaca gaccaatatc accagtgcgg ctggcgtgag
     1501 taccgccaca cagctcggtg gagaaatcgc ccatgctcag cacgcgtaca cgctcatcat
     1561 acttctcgcc gaacagcgcc atcgcacctt tcgctttcgc cgcttcgaga ttcatgatgt
     1621 tggtttcgat cggcaaattg cgacgaatct gtgcgttcac caggtcttcg accgcacgaa
     1681 tctcttccgg tttcatcgct tcgttgtgtg agaagtcgaa gcgcagcacc ttgtcgttaa
     1741 ccagtgaacc tttctgcgat acatgagtcc ccagaacctg gcgcagcgca gcgtgcatca
     1801 ggtgcgttgc ggagtgattc agacgaatac gggcgcgacg agcctcatca atatcagcct
     1861 gcacagcgtc gcccactttc agagaacccg cagcaagttt accgatgtga ccaatcgcct
     1921 ggccgtattt ctgcgtatct tccaccgcga aggagaagtt agcgcctttc agttcgcctt
     1981 tatcaccaac ctgaccgccg gattccgcat agaatggcgt ttgatccagc acgaccacag
     2041 cttcctggcc tgcattgatg gcatcaaccg ctttaccatc aacaaacagc gcggtcactt
     2101 tgccgttcag ttccagatgg tcatagcctt taaattcaga tgcactatca acacggatca
     2161 ttgcgttgta atcggcacca aagccgctgg cttcgcgcgc gcgacgacgc tgctcttcca
     2221 ttgctgcttc aaaaccagct tcgtcaactt tgatgttgcg ctcacgacaa acatcagccg
     2281 tcaggtcaac cgggaagcca taggtgtcgt acagacggaa agccgtttca ccatccagcg
     2341 tatcaccaga aagttttgcc agctcttcat ccagcaacgc cagaccgcgc tccagagtac
     2401 gggcaaactg ctcttcttca gtcttcagca cctgctcaac ctgcgcctgc tggcgtttca
     2461 ggtcttcacc cgcagagccc ataacgtcga tcagcggacc aaccagtttg tagaagaagg
     2521 tttctttcgc gccgagcata ttgccgtgac gcactgcgcg acgaatgata cgacgcagta
     2581 cataaccacg gttttcattg gacggcatta cgccatccgc gatcaggaac gcacaagaac
     2641 gaatgtggtc agcgattacg cgcagcgatt tattgctcag atcggtcgcg ccagtgactt
     2701 tcgctaccgc ctggatcaac gtgcggaaca ggtcgatgtc atagttagag ttaacgtgtt
     2761 gcagcaccgc agcaatacgc tccagaccca taccggtatc tacagacggc ttcggcagcg
     2821 gttccatcgt gccatctgcc tggcggttga actgcatgaa gacgatgttc cagatctcaa
     2881 tgtagcggtc gccgtcttct tccggacttc ccggagggcc accccaaatg tggtcgccgt
     2941 gatcgtagaa gatttcggtg cacgggccgc acggaccagt gtcacccatc tgccagaagt
     3001 tgtcagatgc gtatggcgca cctttgttat cgccgatgcg aataatacgt tcgcgcggga
     3061 tcccgacttc tttttcccag atttcgtagg cttcgtcgtc gctttcatag acggtaaccc
     3121 acagacgctc tttcggcagg gcaaaccatt tttcgctggt cagcagttcc catgcaaact
     3181 gaatggcatc gtgtttgaaa tagtcgccga agctgaagtt gcccagcatt tcgaagaagg
     3241 tatggtgacg cgcggtgtaa ccgacgtttt ccaggtcgtt gtgtttacca cccgcacgca
     3301 cgcagcgttg ggaagtggta gcgcgggaat aattacgctt gtcgagccca aggaacacat
     3361 ccttgaactg gttcatcccg gcgttggtaa acaacaaagt tgggtcgtta tggggtacca
     3421 gggagctgct ggcaactacc tgatgtccct tactatggaa aaagtcgaga aacgcctgac
     3481 ggatctcagc ggtgctcttg ctcataatta tcctgaaatc aagctaacga aatatcgcca
     3541 ccagctccag cgtgtcttaa ccgccgggct ggtaactgaa aagtgggaat aagataagtt
     3601 ttcttgactg ggaagtaaaa taccgtatgc gttcagtcgg caaaatttcg ccaaatatcc
     3661 tggatatctt ccatcagata gccacgatag agcagaaaac gctggatctt aactttttct
     3721 gaaaagacag ttggcaaagg ttcaccatat tttcgcgtcg cctgatcgcg cgccagtgcg
     3781 caccagtcga tgtcacattc acgcatcgct ttttctgtcg cttcgcggga aatacctttc
     3841 tgattcagtt cctgacgaat acgtgcaggt ccataacctt tgcggctacg gctggcgata
     3901 aagcgcgcaa caaatcggct gtcatcgaga tagccatgtt catggcacca ggcaataacg
     3961 cgctcgtaat cttctgccgt agcatcaatc tcttctgggc catttttgcc cataatcggt
     4021 gccgcgagtt tacgtcgcag ttcttgctca ctgtgatcgc gcaccgccag aatgcgtacc
     4081 gcacgatcca acaggcgagc atatgccggg cgacgggatg ttgattctgt catggcatat
     4141 ccttacaact taaaaaagca aaagggccgc agatgcgacc caggttttgc tgataaacag
     4201 atttaatagt caatgagtat gcagaatgtt cccgcaggcc caattcgtta gaggatcgag
     4261 gtgagagtaa tttttcacgc cagtgctggc ctgttcccct caccctaacc ctctccccaa
     4321 aggggcgagg ggaccgtccg tgcacacatt atctattatc atcaataacg aacgattaaa
     4381 aatcttcgtt agtttctgct acgccttcgc tgtcatctac agagaaatcc ggcgttgagt
     4441 tcgggttgct cagcagcaac tcacgtactt tcttctcgat ctctttcgcg gtttccgggt
     4501 tatctttcag ccaggcagtt gcattcgctt taccctgacc gatcttctca cctttgtagc
     4561 tgtaccacgc gcctgctttc tcgatcagct tctcttttac gcccaggtca accagttcgc
     4621 cgtagaagtt gataccttcg ccgtagagga tctgaaattc agcctgttta aacggtgcag
     4681 cgattttgtt cttcaccact ttcacgcggg tttcgctacc caccacgttt tcgccctctt
     4741 tcaccgcgcc gatacgacgg atgtcgagac gaacagaggc gtagaatttc agcgcgttac
     4801 caccggtagt ggtttccggg ttaccgaaca tcacaccaat tttcatacgg atctggttga
     4861 tgaagatcag cagcgtgttg gactgcttca ggttacccgc cagcttacgc atcgcctggc
     4921 tcatcatacg tgccgcaagg cccatgtgag agtcgccgat ttcgccttcg atttccgctt
     4981 tcggcgtcag tgccgccacg gagtcaacga cgataacgtc tactgcgcca gaacgcgcca
     5041 gggcgtcaca gatttccagt gcctgctcgc cggtatccgg ctgggagcac agcaggttat
     5101 cgatatcgac gcccagttta cgtgcgtaga ttgggtccag cgcgtgttca gcatcgataa
     5161 acgcacaggt tttaccttca cgctgcgctg cggcgatcac ctgcagcgtc agcgtggttt
     5221 taccggaaga ttccggtccg tagatttcga cgatacggcc catcggcaga ccacctgccc
     5281 caagcgcgat atccagtgaa agcgaaccgg tagagatggt ttccacatcc atggaacggt
     5341 cttcacccag gcgcatgatg gagcctttac caaattgttt ctcaatctgg cccagtgctg
     5401 ccgccaacgc tttctgtttg ttttcgtcga tagccatttt tactcctgtc atgccgggta
     5461 ataccggata gtcaatatgt tctgttgaaa caattatact gtatgctcat acagtatcaa
     5521 gtgttttgta gaaattgttg ccacaaggtc tgcaatacat acgcagtagc ctgacgacgc
     5581 accgcatcac ggtcgccgct gaagcattcc cgccgggtaa tgccttcacc gcgggcagtg
     5641 gcaaaagcaa accagacggt gccgacaggc ttctcttcac tgccgccatc cggcccggcg
     5701 ataccactaa tagacacggc ataatcagca cgagccgctt tcagtgcacc tatcgccatt
     5761 tccaccacga cgggttcact caccgcgcca tgctgcgcca gcgtctcttc gcgtacgccg
     5821 atcatctgcg ctttggcttc gttactgtag gtgacaaatc cgcgttcaaa ccaggcggag
     5881 ctaccggcaa tatcggtaat cactttcgct acccaaccac cggtacaaga ctcggcagtt
     5941 gttacggttg cgccacgggc tttcagcgcc tgcccaacct gttcgcttaa ctgcatcagt
     6001 tcactgtcag tcatcacaac ctctgtcagt caaaaattta tgccggacaa gatagcactt
     6061 tcgtcaggaa gatggtgttg gcgttgcaat tttacgagga ggattcagaa aaaagctgat
     6121 tagccagagg gaagctcacg cccccctctt gtaaatagct actgtactcg cgccagcgcc
     6181 acggcttgtc ctaactgcca gaccgccatt gcgtaatggg tgctgtggtt gtaacgggtg
     6241 atggtgtaga agttcggcag accgtaccag tactggtagc cggtgccaac atccagacgc
     6301 agcaggctgg cttgttgatg gttgcccagc ggctgctgtg gcgttaaacc tgcggcggca
     6361 agctgcgaaa tgctgtactt agttttgaaa ccatttggca agcctggagc ctgaccgttt
     6421 gccattatcg cgacctgatc gcctttcacc cagccgtgcg ctttgaaata gttcgccacg
     6481 ctaccgatcg catcaaccgg atcccacagg ttgatatgcc cgtcgccgct gaaatctacc
     6541 gcatattgtt tgtaagacga cggcataaac tgtccgtagc ccatcgcccc ggcaaaggaa
     6601 cctttcagat tgagcggatc gtcctgctcg tcgcgcgcca tcagcaggaa ggtttccagc
     6661 tcgccagaga aatactccgc gcggcgtggg tagttaaatg acagcgttgc cagcgcatcg
     6721 aggatgcgag ttttccccat cacgcgcccc cagcgggttt caacgccgat aatcccgacg
     6781 ataatttccg gcggtacgcc atacacctgc cacgcgcgat tcaacgcatc ttcatactga
     6841 ttccagaaaa ccacaccgtt ctgcacgttg tccggcgtaa taaatttttt gcgataacgc
     6901 agccatgcgc cgttcggacc tgatggcggt ttcaccgatg tggttggtgc ctggttatcc
     6961 atcagccgca gtaccgaatc cagacgcttc gcctgggaga gaatttcctg caactgctga
     7021 cgatcgaaac cgtgtttgtt caccattttg tcgatgaact gctgggcatt cgggttatta
     7081 gcgaaatcgc cgcccatctg catcacattg tgctgcggct caagcaggaa gccgccagac
     7141 ggcgttccgg tggtcgtttc agtctcagta ggttttggct tgctgctaca ggcggcaagc
     7201 aacacaaaaa ggggaagcaa tgttacataa cgacgcttga acatgagggg tccatttaac
     7261 agattcaacc aggggcaagt atggtaaagc atcacgcccc gcacaaggaa gcggtagtca
     7321 ctgcccgata cggactttac ataactcaac tcattcccct cgctatactt ttactcaaac
     7381 tttcaaatta aaatatttat ctttcatttt gcgatcaaaa taacactttt aaacctttca
     7441 acctgattag attaggttgc cgtttggtaa taaaacaata aatcctgaag gagagaacaa
     7501 tgatagaaac cattactcat ggtgcagagt ggtttatcgg gctgttccaa aagggcggag
     7561 aggtgtttac cggaatggtg accggcattc ttccgctgtt gattagcctg ctggttatca
     7621 tgaacgcact gattaatttt atcggtcagc atcgtattga acgttttgct caacgttgcg
     7681 ccggtaaccc tgtttcccgt tacctactgt taccgtgcat tggcacgttt gtcttttgca
     7741 atccgatgac cttaagccta ggtcgcttta tgccggaaaa gtacaaaccc agctactacg
     7801 cggcggcctc ttatagctgc cactcaatga atggcctctt cccccatatc aaccctggcg
     7861 aactgtttgt ttatcttggc attgccagcg gtctgacaac gctgaacctg ccacttggcc
     7921 cactggcggt gagttatctg ctggttggtc tggtcaccaa tttcttccgc ggctgggtga
     7981 ccgatctgac caccgccatt tttgagaaaa agatgggcat tcaacttgaa caaaaagttc
     8041 acctggcagg agcaacatca tgacgcgtat tcggatcgaa aaaggaacgg gtggctgggg
     8101 cggcccgctt gagctggaag ctacgccggg caaaaaaatc gtctatatca ccgccggtac
     8161 ccggcctgcg attgttgaca aactggcaca acttactggc tggcaggcta ttgacggatt
     8221 taaagaaggt gaacccgcgg aggcggaaat tggtgtcgcg gtaatcgact gtggcggcac
     8281 attacgctgc ggcatctatc cgaaacgacg tattcccacc attaatatcc actcgacggg
     8341 caagtccggt ccgctggcgc agtacattgt ggaagatatt tatgtctctg gcgtaaaaga
     8401 agaaaacatc actgtagtgg gtgatgcgac accacaaccc tcttccgtgg gccgtgacta
     8461 tgacaccagt aagaaaatca ccgaacaaag cgatggttta ctggcgaagg tgggaatggg
     8521 catggggtcc gccgttgccg tgttgtttca gtctggtcgt gacaccatcg acactgtatt
     8581 aaaaaccatt ctgccgttta tggcattcgt ctcggcgctc attggcatca ttatggcttc
     8641 tggccttggt gactggattg cccacggtct tgctccgctg gcgagccatc cactgggtct
     8701 ggtcatgctg gcgctcatct gctccttccc actgctttca cctttcctcg gcccaggcgc
     8761 agttatcgca caggttatcg gcgtattgat tggcgtgcag attggtctcg gcaatattcc
     8821 gccgcatctg gctttaccgg cactgtttgc catcaacgcg caggcggcct gcgacttcat
     8881 cccggtcggt ttgtcgctgg cggaagcccg tcaggacacg gttcgcgtcg gtgtcccttc
     8941 tgtactggtg agccgctttt taaccggcgc gccgactgta ctgatcgcct ggtttgtctc
     9001 cggttttatc tatcaataga ggctgaaaca tgaccgttat ttatcagacc accatcaccc
     9061 gtatcggcgc gagtgccact gacgccctca gcgaccagat gctcatcacc tttcgtgaag
     9121 gcgcgccagc ggacctcgaa gagtattgct tcattcattg ccacggcgag ttgaaaggtg
     9181 cactccatcc cggtttgcaa ttttcactcg ggcagcatcg ctatccggtg accgctgttg
     9241 gcagcgtggc ggaagacaac cttcgcgaac tgggtcatgt caccctgcgc ttcgatggtt
     9301 taaacgaagc ggaatttccg ggcactgtcc atgtggcagg ccctgtaccc gacgatatcg
     9361 cgccgggatc ggttttgaag tttgaatctg ttaaggagta aaaaatgaat caggttgccg
     9421 ttgtcatcgg tggtgggcaa accttaggcg cgttcctgtg ccacggtctg gctgccgagg
     9481 ggtatcgcgt cgcggttgtc gatattcaga gcgacaaagc cgcaaatgtg gcacaagaaa
     9541 ttaacgccga atatggtgaa ggtatggcgt acggttttgg tgctgacgcc actagcgagc
     9601 aaagcgttct ggcgctctct cgtggggtag atgaaatctt tggtcgcgtg gatttgctgg
     9661 tctatagcgc cggaatagca aaagcagcct ttatcagcga cttccagctc ggcgattttg
     9721 accgttcgct acaagtgaat ctggtgggtt atttcctgtg tgcccgtgaa ttttcgcgtt
     9781 tgatgatccg cgacgggatt cagggtcgca ttattcagat caactcgaaa tccggcaaag
     9841 tgggcagcaa acacaactct ggctacagcg cagcgaaatt tggtggcgtc gggctgactc
     9901 aatcactggc gctggatctg gcggagtacg gcattacggt gcattcactg atgctcggta
     9961 acctgctgaa atcgccgatg ttccagtcac tgttgccaca atacgcgacc aagctgggta
    10021 tcaaaccgga tcaagtcgag cagtattaca tcgacaaagt accgctcaaa cgcggctgcg
    10081 attatcaaga tgtgctgaat atgctgctgt tctacgccag tcctaaggcg tcgtactgca
    10141 ccggacagtc gatcaatgtc accggcggtc aggtgatgtt ctgatcaaca gcggagatcc
    10201 gttaaggatc tccgtgagac tatagaatgc ctgatgcgct acgctcatca ggcatacagg
    10261 acttccgcca ctacattaag gaaaagttat ggtatccgca ctcatcaccg tcgccgttat
    10321 cgcctggtgt gcgcaactgg ccttaggcgg ctggcaaatt tctcgtttta accgtgcctt
    10381 cgacacacta tgccagcaag ggcgggttgg cgtgggccgt tccagcgggc actttaaacc
    10441 gcgggtcgtg gtcgccatcg cgctggacga tcagcagtgc atcgtcgaca ccttgtttat
    10501 gaaaggactg actgtcttcg cccgaccgca aaaaattccc gcaattaccg gcatgcatgc
    10561 gggtgattta cagcccgatg tgatctttcc ccatgatcca ctatcacaga atgctctatc
    10621 attggcgctt aaactgaaac gtggataatt tcgttgtgaa tgttacttgc ttgcgaagtt
    10681 atcattttga aacctaaatc aggtaatcac gcccatgaaa cctcgtcagc gtcaggccgc
    10741 cattctggag tatctgcaaa agcagggtaa atgctcggtt gaagaattgg cgcaatactt
    10801 tgacaccaca ggcacaacca ttcgcaaaga tctggtcatt ctggaacatg ccggaaccgt
    10861 cattcgtact tatggcggag tggtgttgaa taaagaggaa tccgatccgc ctatcgatca
    10921 taaaacgctc atcaacaccc acaagaaaga gctgattgca gaagctgccg ttagttttat
    10981 ccatgatggc gattcgatca ttcttgatgc tggcagtacc gttttgcaga tggttcccct
    11041 gctctcgcgc tttaataaca tcacggtgat gaccaacagc ctgcatatcg tcaatgcgct
    11101 atccgaactg gataacgaac aaactatcct gatgccaggc ggaacatttc gcaaaaaatc
    11161 ggcctcattt cacgggcaac tggcagagaa tgccttcgag catttcacct tcgataaatt
    11221 gtttatgggc accgacggca tcgatctcaa tgcgggcgta accacgttca acgaggttta
    11281 taccgtcagt aaggcaatgt gcaatgccgc gcgcgaagtg attttgatgg cggactcatc
    11341 aaagtttggc cgtaaaagcc ccaacgtagt ttgcagtctt gaaagcgtcg ataagctaat
    11401 taccgacgca ggtatcgatc cggcgtttcg tcaggcgctg gaagagaaag ggatcgatgt
    11461 gatcataacc ggagagagca atgagtgaag cactactgaa cgcgggacgt cagacgttaa
    11521 tgctggagtt gcaggaagca agccgtttac cggaacgtct gggcgatgat tttgttcgcg
    11581 ccgccaatat catcctgcac tgcgaaggca aagtggtggt ttcgggaatt ggcaaatcgg
    11641 gccacattgg taagaaaatc gccgcaacac ttgccagtac cggcacaccg gctttttttg
    11701 tccatccggc agaagcactg cacggcgatc tggggatgat tgaaagccgc gatgtgatgc
    11761 tgtttatctc ttactccggt ggcgcgaagg aactggatct gattattccg cgtctggaag
    11821 ataaatctat cgcgctgctg gcgatgaccg gcaaaccgac gtcaccgctg ggcctggcgg
    11881 cgaaagcggt gctggatatc tccgtagaac gcgaagcctg cccgatgcac cttgcgccga
    11941 cctccagcac cgtcaatacc ctgatgatgg gtgacgcgct ggcgatggcg gtcatgcagg
    12001 cgcgcggttt taatgaagaa gattttgccc gctcccaccc agctggggca ctgggcgctc
    12061 gcttgctgaa taaagtgcat catctgatgc gtcgtgatga cgctatccca caggtagcgt
    12121 taaccgccag cgtgatggat gcgatgctgg aactcagcag caccggtctg gggctggtgg
    12181 cggtatgtga cgctcaacaa caggtacaag gcgtctttac cgacggcgat ttacgtcgct
    12241 ggctggtagg cggcggcgca ctcaccacgc cagttaatga agcgatgacg acaggcggca
    12301 ccacattaca ggcacaaagt cgcgccatcg acgccaaaga agttctgatg aaacgcaaaa
    12361 tcactgccgc accagtggta gatgaaaacg gcaaactcac cggcgcaatt aacctgcagg
    12421 atttctatca ggccgggatt atttaatcct tcaatcccag acgtttcgcc agccgatgca
    12481 ggttggcgac gtcagtttcc agcattcgcg cacaggctgc ccagttgtga tgattttgtg
    12541 ccagtgcctg gcgaatagtt tcacgctgga acgcttctgt cgcttcacgc aggttttgct
    12601 taacaacggg caccgccgcc gcttctggcg gcggcaacgt cacctcagga aaagcaaaat
    12661 gttgcgcctc aagaatcact tcatcgccgt tgcgggtggc tctcgccagc actaccgccc
    12721 gatgaatagc atgttccagt tcgcgcacgt ttcccggaaa actgtagtgt tgcagtaaat
    12781 ttcgcgcccc ggcacttaat accacgcggg agagcccctg ccgcaaacga cactgctcgc
    12841 agaaataccc cgccagcaga atgacatcat cgccccgctc acgcagcggc ggcaccgaaa
    12901 gtggaaacac gctcaggcga tgaaacaaat cggcgcggaa tcgccctgcc agcacctctt
    12961 cgcgtaagtc gcggttagtc gccgccagca cgcgcacatc gacccgcaaa ctacggtcat
    13021 tgccaacgcg ctgaatatcg ccatactgca acaccctcag cagcttggcc tgcaatgcca
    13081 acgacaactc gccgatctca tcgagaaaca gagtaccgtt atccgccatt tcaaacttcc
    13141 cgctgcggtt actgatagcg ccagtaaacg ctcctttcac atgcccgaac aactcacttt
    13201 ccgccacact ttccggcagt gcggcacagt tgagatagac cagcggattc accgcccgtg
    13261 gcgaggcttc atgaatcgct ttcgccacca gctccttacc ggttcccgtc tcaccgctga
    13321 tcagaacgtt gagatcggac gccgccacaa tctcaatctc ttttttcagt tgcgtcatgc
    13381 caggggacaa gccaatcatc tgcgtctgtt tcaccgcttc aaacggcgtg gcatcgcctg
    13441 gcagcatatt ctggctttcc agttgttcaa tcagcaacgc attgcttaac gctcccgccg
    13501 ccagcgcggc aatcagccgt aactcttcgt cgctgaaaac atcgaactga tcgggctgca
    13561 tcccgtcgag cgtcagtgcg ccgatcaggt tttgtccgac aaacaatggc agaccaacgc
    13621 aggcgtgaac cttcagactc tcctgcccag gaatcaaacc gtcataggga tcgggcaatt
    13681 cgctgtctgc gggaaagcgc accacatccc cggcgcgggc aatcgcttcc agccgtggat
    13741 gcccttccag cgcaaagcgt ctaccgagta catcctttgc cagaccgtcg atggcaagcg
    13801 gaataaactg ccgcgaatcg taacgtagca acgcagacgc atcgcactcc agcacctgac
    13861 gtagcgtggt gatcaggcgc tgaaaacgat cctgatgacc aatcccacgc tgcaattcga
    13921 tggcgatatt cgccagcaca tcaacggaaa aactcatctt tgcctcactg tcaatttgac
    13981 tatagatatt gtcatatcga ccatttgatt gatagtcatt ttgactactc attaatgagc
    14041 ataattttat ttatagagta aaaacaatca gataagaaac tggcacgcaa tctgcaatta
    14101 gcaagacatc tttttagaac acgctgaata aattgaggtt gctatgtcta ttgtggtgaa
    14161 aaataacatt cattgggtgg gtcaacgtga ctgggaagtg cgtgattttc acggcacgga
    14221 atataaaacg ctgcgcggca gcagctacaa cagctacctc atccgcgaag aaaaaaacgt
    14281 gctgatcgac accgtcgacc ataaattcag ccgcgaattt gtgcagaacc tgcgtaatga
    14341 aatcgatctg gcggatatcg attacatcgt gattaaccat gcagaagagg accacgccgg
    14401 ggcgctgacc gaactgatgg cgcaaattcc cgatacgccg atctactgta ctgccaacgc
    14461 tatcgactcg ataaatggtc atcaccatca tccggagtgg aattttaatg tggtgaaaac
    14521 tggcgacacg ctggatatcg gcaacggcaa acagctcatt tttgtcgaaa caccaatgct
    14581 gcactggccg gacagcatga tgacttacct gacaggcgac gcggtgctgt tcagtaacga
    14641 tgctttcggt cagcactact gcgacgagca tctgttcaac gatgaagtgg atcagacgga
    14701 gcttttcgag cagtgccagc gttactacgc caatatcctg acgccgttca gccgcctggt
    14761 aacgccgaaa attaccgaga tcctgggctt taacttacca gtcgatatga tagcgacctc
    14821 ccacggcgtg gtctggcgcg ataacccgac gcaaattgtc gagctgtacc tgaagtgggc
    14881 cgctgattat caggaagaca gaatcaccat tttctacgac accatgtcga ataacacccg
    14941 catgatggct gacgctatcg cccagggaat tgcggaaacc gacccacgcg tggcggtgaa
    15001 aattttcaac gtcgcccgaa gcgataaaaa cgaaatcctg accaatgtct tccgctcaaa
    15061 aggcgtgctg gtcggcactt cgacgatgaa taacgtgatg atgccgaaaa tcgccgggct
    15121 ggtggaggag atgaccggat tacgcttccg taacaaacgc gccagtgctt tcggatctca
    15181 cggctggagc ggcggtgcgg tggatcgcct ttccacgcgc ctgcaggatg cgggtttcga
    15241 aatgtcgctc agcctgaaag ccaaatggcg accagaccag gacgctctgg agttatgccg
    15301 tgaacacggt cgcgaaatcg cccgtcagtg ggcgctcgcg ccgctgccgc agagcacggt
    15361 gaatacggta gttaaagaag aaacctctgc cacgacgacg gctgacctcg gcccacgaat
    15421 gcagtgcagc gtctgccagt ggatttacga tccggcaaaa ggcgagccaa tgcaggacgt
    15481 tgcgccagga acgccgtgga gtgaagtccc ggataacttc ctctgcccgg aatgctccct
    15541 cggcaaagac gtctttgacg aactggcatc ggaggcaaaa tgagcaacgg cattgtgatc
    15601 atcggttcgg gcttcgccgc ccgccaactg gtgaaaaata ttcgcaaaca ggacgccact
    15661 attccattaa ccctgattgc cgccgacagc atagatgagt acaacaaacc cgacctcagc
    15721 catgttatca gtcaggggca acgtgccgac gaccttaccc gccagacggc gggtgaattt
    15781 gccgggcagt ttaatctgcg cctgtttccg cacacctggg tgacggatat cgatgccgaa
    15841 gcccatgtgg tgaaaagcca gaataatcag tggcaatacg acaagttagt gctggcaacc
    15901 ggtgccagcg cctttgtccc gcctgtgcct gggcgtgagt taatgctgac gttaaatagt
    15961 cagcaagagt atcgcgcctg tgaaacgcaa ctgcgggatg cccgacgcgt gttgattgtc
    16021 ggcggtggtt tgattggtag cgaactggcg atggattttt gtcgtgcagg caaagcggtc
    16081 acgctaatcg acaacgctgc cagtattctg gcgtcattaa tgccaccaga agtaagcagc
    16141 cgcttgcagc atcggttgac ggagatgggc gttcatctgc tgttgaaatc tcagttgcag
    16201 gggctggaaa aaacggattc tggcattctg gcaacgctgg accaccagcg tagtatcgaa
    16261 gtggatgcgg taattgctgc caccggactg cgcccggaaa cagccctggc acgacgcgcc
    16321 gggctgacga ttaatcgcgg cgtttgcgtc gatagttatc tgcaaaccag taatgccgat
    16381 atttacgcgc tgggcgattg cgcggaaatt aacggtcagg tattgccgtt cctgcagccg
    16441 attcaactta gcgggatggt gctggcaaaa aatcttctcg gcaataacac gccgctgaaa
    16501 ctaccggcga tgctggtgaa aatcaaaacg ccggaattac cgctgcatct ggcaggcgaa
    16561 acccagcgtc aggatttacg ctggcagatt aataccgaac gccagggaat ggtggcgcgc
    16621 ggcgttgacg atgctgacca gcttcgcgcc tttgtggtca gtgaggatcg gatgaaagag
    16681 gcatttgtat tgttgaaaac gttgccggtg taggtgggct actgtgccta aaatgtcgga
    16741 tgcgacgctg gcgcgtctta tccgacctac ggggacgcat gtgtaggccg gataaggcat
    16801 ttacgccgca tccggcaagg gtgtccaaat gcaacacgct ttatccgttc tggacttcac
    16861 ccgctaacca acgcgccgca gcaataaccc cctgccccag agacaaaccg ccatcacccg
    16921 ccggtaaact ctgtggaaag agcaatgtga aatcagcgag ataatgcgcc agacgtgcac
    16981 gcagcaaacg gttatgaata accccgccgc taaataccag cgtagtgata ccacgcatcg
    17041 tggcctgctc acgcatcaac gcggcaaaac cctgcgccag cgcatcatga aacgcccacg
    17101 cgcgttgatt aaccggtgcc tgccagttca gccactgctg ccagaaagtg gcgagatcca
    17161 gttgattgtc cacccgcggc atcgtcaccg gatgcgtcac tccgtcgcat gaggctgcga
    17221 gcgcctccag agcacaagcc gcttcacctt cataacttaa cgtggctggc gcacagccca
    17281 gtgccgccgc cactgcatcg aacaaccgcc cacacgatga tgccagcggc gcgttaattc
    17341 cacgctcaat ggcccgcgcc agtacgctcc agttttgctg ttgcacactt gccgtttcag
    17401 ggtaattctg ccactccggc acaaagcgca ggcactgcgc cagcaggttt cgccacggct
    17461 gcttcgccgc caaatcgcca cccggaagcg ccactgcagg caagccgccc aggtgctcac
    17521 attcgcgata gttcacccgc aggcactcgc cgccccacaa agcgccattc tcccccatac
    17581 cgataccgtc gagcgtcaaa gcaatgacat caccgccatc cagcggccac tgatgctctg
    17641 ccagacacgc cgctgcatgg gcatgatgat gcagtaccgt ttgcgtcggc agattcattt
    17701 cgctcgccca ctggctggag acatagcccg gatgcacatc atgcacaatg tattgcggag
    17761 tgaaatcgta gatgttttgc atcaggcgta acgcttcgcg ccactgcatc tggatgccat
    17821 cgtcacttaa atcgcccaga tgctgactca acaccacttg ttcaccgcgc accaggcaga
    17881 aggtattttt cagatccgcg ccaagacaca gcacaggcgg aacattttta aagcccggag
    17941 gcaaaaccag cgcatccggc acataccccc gcgaacggcg cagcatttcg ccgctttcgc
    18001 gcaccactga atcatccatc cgctgcacga tgtcgcggtt atgtagcaag aatccgtcgg
    18061 caatgccctg caaatccgcc agcgcctgtt cgttgctgat agctggtggt ttaccgctca
    18121 ggttgccgga ggtcatcacc agcgggcatt gcagttcctg taacagcaaa tgctggagcg
    18181 ggttcgcagg caacatcacc ccgacttcgt taaggccagg ggcgatatca tcacaaagct
    18241 caggtacgta ttttttatcc accagcacaa tcggcgcagc gggcgtggta agcaactggc
    18301 gcgcagcgtc tggtaaactt tctgccactg gcaacatgac tgccagcggt ttcgccgggc
    18361 gatgtttgcg ctcccgcagt gtcgccaccg cgttactgtt acgtgcatcg caggcaagat
    18421 gaaatccgcc aatccctttg atggcgacaa tgttgcccat ttttaactgt gcgatagctg
    18481 cctgtaatgc cgcctcttgt tctgcatgtt caccatgact tacccattca agatgcgggc
    18541 cacactccgg gcaggccacc ggctgggcgt ggaagcgacg atcgagcggg tcgcggtact
    18601 ctttgtcaca ggccggacat agcggaaacg ccgccatcac ggtaaacggg cggtcgtaag
    18661 gcatcgcgcg aataatggtg aaacgcgggc cgcagtgggt acagttgata aacggataac
    18721 gataacgccg ctcgcctggg gtattcattt cggcaaggca agcagggcaa gtggcggcat
    18781 ccgggacaat ttgcgtattc atggtgccgc cggtgctctg gcggatggtg aactcggtgg
    18841 gcagttgtga ccagataaac ggctcacgct cgacgctatc aatacgcgcc agtggcggac
    18901 agtgctgatg caattgaaca agaaacgttt ccggatcttc cagcagccgg acttctacgc
    18961 catcgccgtc attacataca tcgccgtgaa gatttaattg ctgtgccagc tgccagacaa
    19021 acggacgaaa accgacgccc tgcactttgc cacgaatacg cagttggaca ccgcaaaatg
    19081 tgttttttgc cattgagtta ttcccgccat catgaattgc gtaacccgcc ctgccggaca
    19141 cgacagcgtc gtatccggca gtctcaggtc ggcgataccg cccgctccgt attctacgaa
    19201 tatttccggg aattcctttg atgccagaac agttctgtaa gatttttaga acatcagcgc
    19261 cgtacggcgg cgtttttctg cgctcagttg ttcaagttta ttacgatcga cacaaatcag
    19321 cgcatgggtc gggcaagccg ccatacacgc cgggccgtct tcacgatggt tgcacaggtc
    19381 gcatttattg gcttcggctt tgtcagcccg tacattcaga cccgcgccgc tgttgcgaat
    19441 cacaggacgt accaccactt ccatcgcgcc atacgggcaa gccaccacgc aggttttgca
    19501 accaatgcaa cgttcctgca tcacatgaac aaacccttta tcacagctga tagcaccatt
    19561 cgggcagacg ttagcgcacg gtgcatcttc acactgacgg caaactgtcg ccgtggaaat
    19621 gttcacacct ttaatgacat ggatacgcgg taaaaatgtt tccggggtca gcgatgcaca
    19681 gtcctgattt tcctgatgag aaaccacgca cgccacttca caggtacggc aaccaataca
    19741 tttactcgcg tcagcaatga tgaaacggtt catcaaattc
//
LOCUS       DADMGC010000076        19639 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.076, whole genome shotgun sequence.
ACCESSION   DADMGC010000076 DADMGC010000000
VERSION     DADMGC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19639
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.076"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..1224
                     /locus_tag="JNZ73_002566"
     CDS             <1..1224
                     /locus_tag="JNZ73_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708695.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY8732805.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGIIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="yggF"
                     /locus_tag="JNZ73_002567"
     CDS             1221..2186
                     /gene="yggF"
                     /locus_tag="JNZ73_002567"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY8732806.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVICEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="JNZ73_002568"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="JNZ73_002568"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HAY8732807.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="JNZ73_002569"
     CDS             2714..3427
                     /locus_tag="JNZ73_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HAY8732808.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="JNZ73_002570"
     CDS             complement(3399..4076)
                     /locus_tag="JNZ73_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY8732809.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="JNZ73_002571"
     CDS             complement(4070..4747)
                     /locus_tag="JNZ73_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAY8732810.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTCSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5442)
                     /locus_tag="JNZ73_002572"
                     /pseudo
     CDS             complement(4735..5442)
                     /locus_tag="JNZ73_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
     gene            complement(5443..6021)
                     /locus_tag="JNZ73_002573"
     CDS             complement(5443..6021)
                     /locus_tag="JNZ73_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAY8732811.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="JNZ73_002574"
     CDS             complement(6044..6475)
                     /locus_tag="JNZ73_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY8732812.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELEFSRQPCALSGYDELHISPVK"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="JNZ73_002575"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="JNZ73_002575"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HAY8732813.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHSEAHIAAGAKKVLFSHPGSNDLGATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="JNZ73_002576"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="JNZ73_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAY8732814.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="JNZ73_002577"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="JNZ73_002577"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAY8732815.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /locus_tag="JNZ73_002578"
     CDS             10564..11424
                     /locus_tag="JNZ73_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HAY8732816.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="JNZ73_002579"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="JNZ73_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HAY8732817.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQHIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="JNZ73_002580"
     CDS             12291..13031
                     /locus_tag="JNZ73_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HAY8732818.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..13707
                     /locus_tag="JNZ73_002581"
     CDS             13198..13707
                     /locus_tag="JNZ73_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8732819.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPL"
     gene            13831..14093
                     /locus_tag="JNZ73_002582"
                     /pseudo
     CDS             13831..14093
                     /locus_tag="JNZ73_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001451535.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="JNZ73_002583"
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="JNZ73_002583"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HAY8732820.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="JNZ73_002584"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="JNZ73_002584"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HAY8732821.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="JNZ73_002585"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="JNZ73_002585"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HAY8732822.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="JNZ73_002586"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="JNZ73_002586"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HAY8732823.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLGKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKMDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMTEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRAARDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVAGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
BASE COUNT         4716 a         4910 c         5279 g         4734 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg ggattattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggccgcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tctgcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgactctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa accgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcagacgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggtgctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agtcgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ctatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggcgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgaccacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct acaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagctcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaactcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc caggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgggta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagcca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctagat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat agcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcggcg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcccttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ttggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcacattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgtgattg ggctttctcg tttctcgccg ttataaagag
    13741 cgtcaagagc aagagctgct tcaggagctc ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggtgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgcg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagcc
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgtaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttattgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgacgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccgctacg tgaacgtcgt tttctacggc caggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggc ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcggtca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tccattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttaccc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagatt
//
LOCUS       DADMGC010000077        18260 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.077, whole genome shotgun sequence.
ACCESSION   DADMGC010000077 DADMGC010000000
VERSION     DADMGC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.077"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(61..819)
                     /gene="aroD"
                     /locus_tag="JNZ73_002587"
     CDS             complement(61..819)
                     /gene="aroD"
                     /locus_tag="JNZ73_002587"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAY8732824.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="JNZ73_002588"
     CDS             complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="JNZ73_002588"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HAY8732825.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1728..2942)
                     /locus_tag="JNZ73_002589"
     CDS             complement(1728..2942)
                     /locus_tag="JNZ73_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8732826.1"
                     /translation="MYFSYFLHGISVITLAQNMTSLAEKFSTDNAGIAYLISGIGLGR
                     LISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLTLAYGLAVCVGIANSALD
                     TGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYMLLNNIWYGYGLIIPGILF
                     VLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSSVLFGVAAFSTFYVIVVW
                     MPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKMVRPIWANVFNSALATIT
                     AAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFPKSKAKVTSIYMMMGGLA
                     NFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRYYRVFIIPENDVRFGERK
                     FSTRLNTIKHRG"
     gene            complement(3182..4327)
                     /locus_tag="JNZ73_002590"
     CDS             complement(3182..4327)
                     /locus_tag="JNZ73_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8732827.1"
                     /translation="MGVILMSLNMASLETLCQTNAAGVSIVISSLGIGRLSVLLFAGL
                     LSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFGFLAGMANSFLDAGTYPSLME
                     AFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWFGWSFMIAAGIMFINALFLYR
                     CTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGYISMATFYLVSQWLAQYGQFV
                     AGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTTLLMLYTFISFIALFTVCLHP
                     TFYVVIIFAFVIGFTSAGGVVQIGLTLIAERFPYAKGKATGIYYSAGSIATFTIPLIT
                     AHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHSLKENVAPGG"
     gene            complement(4486..4842)
                     /locus_tag="JNZ73_002591"
                     /pseudo
     CDS             complement(4486..4842)
                     /locus_tag="JNZ73_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            complement(4978..5085)
                     /gene="rprA"
                     /locus_tag="JNZ73_002592"
     ncRNA           complement(4978..5085)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="JNZ73_002592"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="JNZ73_002593"
     CDS             complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="JNZ73_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HAY8732828.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAILAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6772..9828
                     /locus_tag="JNZ73_002594"
     CDS             6772..9828
                     /locus_tag="JNZ73_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY8732829.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVVLIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9825..10235
                     /gene="menI"
                     /locus_tag="JNZ73_002595"
     CDS             9825..10235
                     /gene="menI"
                     /locus_tag="JNZ73_002595"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HAY8732830.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10335..10523
                     /locus_tag="JNZ73_002596"
     CDS             10335..10523
                     /locus_tag="JNZ73_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732831.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11071..11439
                     /gene="sufA"
                     /locus_tag="JNZ73_002597"
     CDS             11071..11439
                     /gene="sufA"
                     /locus_tag="JNZ73_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HAY8732832.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEIDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11448..12935
                     /gene="sufB"
                     /locus_tag="JNZ73_002598"
     CDS             11448..12935
                     /gene="sufB"
                     /locus_tag="JNZ73_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAY8732833.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12945..13691
                     /gene="sufC"
                     /locus_tag="JNZ73_002599"
     CDS             12945..13691
                     /gene="sufC"
                     /locus_tag="JNZ73_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAY8732834.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETHDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13666..14937
                     /gene="sufD"
                     /locus_tag="JNZ73_002600"
     CDS             13666..14937
                     /gene="sufD"
                     /locus_tag="JNZ73_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAY8732835.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14934..16154
                     /gene="sufS"
                     /locus_tag="JNZ73_002601"
     CDS             14934..16154
                     /gene="sufS"
                     /locus_tag="JNZ73_002601"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HAY8732836.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQAQGCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16167..16583
                     /gene="sufE"
                     /locus_tag="JNZ73_002602"
     CDS             16167..16583
                     /gene="sufE"
                     /locus_tag="JNZ73_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HAY8732837.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16732..17736
                     /gene="ldtE"
                     /locus_tag="JNZ73_002603"
     CDS             16732..17736
                     /gene="ldtE"
                     /locus_tag="JNZ73_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HAY8732838.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17800..18036)
                     /locus_tag="JNZ73_002604"
     CDS             complement(17800..18036)
                     /locus_tag="JNZ73_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HAY8732839.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4807 a         4403 c         4689 g         4361 t
ORIGIN      
        1 cggttaaaaa tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc
       61 ttatgcctga tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg
      121 cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccagccag
      181 acgagaaatt acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg
      241 ctcttgcatc tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat
      301 cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat
      361 ttcttcggct tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg
      421 cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc
      481 gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt
      541 ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc
      601 tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc
      661 ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc
      721 ttcggatttc acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc
      781 cgcaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg
      841 gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt
      901 gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat
      961 cgttttgcaa ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac
     1021 gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt
     1081 ctcaaggggt ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc
     1141 ttcagcaaag gcttgctgat cggcgagatc ggtaaccgtg acgacacaat cggtgttttc
     1201 gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag
     1261 tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc
     1321 ggcccctaac agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg
     1381 aatatggccc gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt
     1441 gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag
     1501 ttgtttgttc ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat
     1561 tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc
     1621 tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag gataagccat
     1681 caatccaatc aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc
     1741 ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa accgcacatc attctcagga
     1801 ataataaaaa cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc
     1861 gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta
     1921 atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg
     1981 gctttgcttt tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca
     2041 gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg
     2101 tagataatgg cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc
     2161 cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg
     2221 taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat
     2281 ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc
     2341 gatgaaacac cttccagcca gactaacggt ttgctgttca tttgcggtaa ttcattagct
     2401 acgctggcgt ccaccaactg gctggggaat ttgcttttca acagcatcag cgtgatcagt
     2461 acaaacagaa tacccggaat aatcagccca tagccgtacc agatattatt gagcaacata
     2521 tagctcacca gcattgggta gaacatttgc ccaaatgaca ccatcgcttt aaccagtatg
     2581 accgccgaac cagaggcttt cggaaagcat tccatgagcg cggggtagcc acccgtatcc
     2641 agcgctgagt tagcgatacc tacgcacact gccagaccgt aggcgagagt taaattcggg
     2701 caagcgggaa taccaaagaa gaatagcaga tacattatta ctgccattaa tatcaccgcc
     2761 cgacgaccaa acttatcgga gatcacaccg aagaataaaa tactgatcaa tcgccccaaa
     2821 ccgataccgg aaattaaata ggcaatgccc gcgttgtcag tggaaaactt ttctgccaga
     2881 gatgtcatat tttgggcaag cgtaataaca ctaatgccgt gcaggaaata gctgaagtaa
     2941 atacaaagaa cagccaggat aaatggcgtg ctgaaagata atttcgttac cgtacgtatt
     3001 gttctccgac ggtttacata attactatac tgaagatgat tttccatggg tacatggcat
     3061 aaagtttatg tttccaggaa tattgcatgt catagtttgt ttttgatcgc gattttcctt
     3121 agttagcgca gaaagagtta tttatccgta agatcttgca tgacctgaaa agaatattgc
     3181 attacccacc cggagcgaca ttttccttta gcgagtgatg ccgcgttttt ttgcggctgc
     3241 gtaagccgat aaacagtgcc agtaaaaaac cgatggcagt atcgaaccac ataatatcgg
     3301 caatacttct ttgggacaga tgagccgtaa tcaacggaat agtaaaggtc gcaatactgc
     3361 ccgcactgta atagatccct gtagctttac ctttagcgta agggaaacgt tcagctatta
     3421 acgtcaggcc aatttgcaca acacctccag cagaggtaaa accaatgaca aaagcaaata
     3481 ttatcaccac ataaaatgtg ggatgcaggc agacggtaaa cagagcaata aatgagataa
     3541 aggtgtacag catcagtaat gttgttgggc gaacggtatt acgaatgagt ggagcggtaa
     3601 taaatacaca aagcagcgaa cccacggtgt agatactgag tagtttgatc gacatagtgt
     3661 atgacatgcc tgcaacaaat tgtccgtact gtgccagcca ctggctaacc agataaaacg
     3721 ttgccattga gatatagcca tataaggtat aactggctaa atcgataatt gaacagcgat
     3781 gttccgtaga gctggtggtt ttctttatga caggtaagcg acgacccgga tggggtggga
     3841 acgtacaacg gtataaaaac agagcgttaa taaacataat gcctgcagca atcataaagg
     3901 accaaccgaa ccacagttca gcccacacta acaggctaat gattagcggt aataaaaatt
     3961 gtccgctgga aacaaatgct ttaattaaaa tattggctgt cccaggtgag cgtggaaaag
     4021 cttccatcaa actgggataa gtgcctgcat cgagaaaact gtttgccatt cccgccagaa
     4081 agccaaaaac ataagcgata atgatgttat tggtctgcag gatgccaaaa aagaaggcca
     4141 tatagcagca cattccgagc atgataaaag ggcggcgacc aaagcgatcg gataataatc
     4201 ctgcaaaaag caagacactt aatcgaccaa tgcccagcga tgagataact atcgagacac
     4261 ccgcggcatt agtctgacaa agtgtctcca gcgaggccat attcaggctc atcaaaatga
     4321 cgcccatacc atgcaccagg taataaaata caacccaagt gcggtagggt aatagggatt
     4381 tttcataggt tctacttagc aaaggtgctt taaaaagtgt agcgatgttg tgtcagtatc
     4441 cattcattat tgttgtaaca acgacaattc ctttctgtgg aaaaattaac aaagccgctc
     4501 cagatcgtgt gcatataatt cagcggctac cgattgatag atcttccagt caataaaatt
     4561 gccatcaggg tagacttgtt gaaacgcggt taagtcgggg aaaaaacgca tggtgttgtt
     4621 gccgatgcgg ttatttattt tctctatgat ggcatgaaga tgtttttttc gttactgacg
     4681 catagccaac agttgctcga ctacacaatc aggaatggcg catttgccat tttcccactg
     4741 acgccagctt tcactgtcac cggtctgggc aatccaggtc gcgcattcat caatagtcat
     4801 ggcaaaaata tggcggagtg cctggagttc ataagcgttc atcgtgtaat ggctgcatga
     4861 aataaacaat aaaatataaa taacattatc agcctgctga cggcttgaag agagtcacag
     4921 tatcttgtgc aacattattg gaaagagtga ggggcgaggt agcgaagcgg aaaaatgtta
     4981 aaaaaaagcc catcgtggga gatgggcaaa gactacacac agcaattcgt tgtttcactc
     5041 aggggatttc catgcttata aatcaatatg ttgatttata accgtgagct aatagtaggc
     5101 atacggactt tttgcgtcga tcagattcgt ctcattagtt taaaaactgt aaagaatttg
     5161 cgacaggtac taccagctga atggcggtaa gcaggaaaat atggggaaga gcaagcaaat
     5221 atcaattagc ggtgattaac aatcaggcgg cagagccgcc tgatgagaaa ttacttattc
     5281 ggtttctcga tttcgccaag ttcttcgaga atttcttccg gttggtccgt cggcggcggg
     5341 acttcttcca cccacgcggc aaacagacgc caggaaacgg ctaacagaac cggaccaata
     5401 aacagaccga tcatcccgaa agcaatcaaa ccaccaataa cgccagagag gatcaggatc
     5461 agcggtaaat cggcacccat gcgaattaac attgggcgga tgacgttatc cagcgtgcca
     5521 accacaccgc tccacactaa caataccgtt ccccaggtgg tatcgccggt ccagtagagc
     5581 cagataatcg ccggaatcag taccggcaac gggccaagct ggacaaggca ggagaggatc
     5641 attaacaccg ttagcagcgt tgcataaggt acgccggata cggcgaggcc gataccgcca
     5701 agcactgcct gtactaacgc cgtcaccacc acacccagcg ccaccgcgag gatagcctgt
     5761 gccgccagca gcacggcggc atctccgcga acgcctgcca gacgggttgc aaaatggcga
     5821 atgccttgtg ccacctgttc accgcgccag tacagcaggg cactgaagag aagcatcagc
     5881 gcacaatgca ccataaagcg cccgatatgc gccgcctgcc caacgaacca agtggtggtg
     5941 gtgccaatat aagggcggac tttcgccatg atcgccgtac cccccatatc cagcaagttg
     6001 tgccagcctg catacagctt cgcgccaatc accggaatgg tattaagcca cgccagatcg
     6061 ggtaacgtca tgtcgccgct ggaaatggct ttaattagcg ggccgctgcc gtcgacgata
     6121 ctgttaacca gcaaagcaat agggatgata aacaccatca ctaataacag cgtcatcacc
     6181 agaacggcga gagagcggcg gccaaacatg atcttttgca aacgtaacaa taccggccag
     6241 gtggcgataa ccaccgtacc ggcccatgca aagccgagaa taaagggttg aacaatccac
     6301 agacatgcca caatcatgat ggctaaaaac agcaccgaaa gcagaatttg tgcgacatcc
     6361 ctgggctgac gaacatttac cataactact tttcaccttt tttgtgcgtc aaatcgtcga
     6421 cgcgacgtgg acacgcgaaa ctcacctgca taatgatgag caatttcagc ggtttttaac
     6481 aggccgattc tgtccttaat tctgatgggc gtgttagaaa aaaatgtggt acaacaactc
     6541 aggcaacacg caaacgattt actcgtcgta tttcaagttg catgtgctgc ggctacgttc
     6601 gttcacccca gtcacttact attgtaagct cctggagttc actcgcttgc cgccttcctg
     6661 caactcgaaa tccatagagt aaataaacat cacctttcag tgcgctattg cagccgcctg
     6721 aaagaatagt gtatcgacct acatcacaga ccgcaggaaa gggtcaatat aatgattcca
     6781 cagatttccc aggcacccgg cgtcgttcaa ctggtgctta attttttgca agagctggag
     6841 caacaaggtt ttaccggcga tgcggcgaca agttatgccg atcgtctgac aatgtcgacc
     6901 gacaacagta tttaccaact tctccccgat gcggtggtat ttccgcgttc aaccgcagat
     6961 gtggtgctga tcgcccgtct tgccgcgcag gaacgctatt catcgctgat ctttaccccc
     7021 cgcggcggcg gcaccggcac taacggtcag gcgctcaacc aggggattat tgttgatatg
     7081 tcccgccata tgaaccgcat catcgaaatt aaccctgaag agggctgggt gcgcgttgag
     7141 gccggggtga taaaagacca actcaatcag tacctgaaac cgttcggcta cttttttgcg
     7201 ccggaacttt cgaccagcaa ccgggcaacg ctcggtggga tgatcaatac cgatgcatcc
     7261 ggtcagggat cgctggtcta tggcaaaacg tcagatcacg tacttggcgt acgcgcggtg
     7321 ttgttggggg gcgatattct cgatacgcaa cctttacccg tcgagctggc ggaaacgctg
     7381 ggtaaatcca ataccacaat cgggcgaatt tataacacgg tttatcaacg ttgccgtcag
     7441 caacgccagt taatcatcga caacttcccc aaacttaacc gctttcttac cggttacgat
     7501 ctgcgtcatg tctttaacga tgagatgacc gagttcgacc tgacgcgcat tctgacgggt
     7561 tcagaaggga cgctggcctt tattaccgaa gcgcggctgg atattacgcg cttgcctaaa
     7621 gtgcgccgtc tggtgaatgt caaatatgac tcttttgact ctgcgctgcg taacgcgccg
     7681 tttatggttg aggcgcgggc gctttcggta gagacggtgg actcaaaagt gctgaatctg
     7741 gcgcgggaag atattgtctg gcattccgtc agcgagttga ttaccgatgt gcctgacaaa
     7801 gagatgctcg gactgaacat tgtggaattt gctggtgatg atgaggcgct gattgacgag
     7861 cgagttaatg cactctgtgt gcggcttgat gagctgattg ctagccaaca ggcaggtgtg
     7921 attggctggc aggtgtgccg cgagctggcg ggggttgaac gtatctatgc gatgcgcaaa
     7981 aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta agccaattcc gtttgctgag
     8041 gatacctgcg taccgccgga acatctggcc gattatattg ctgaatttcg cgcgctgctc
     8101 gacagccacg gcttaagcta cggtatgttc ggtcacgtcg acgcaggtgt cttgcacgtc
     8161 cgtccggcac tggatatgtg cgatccgcaa caagagattt tgatgaagca aatctctgat
     8221 gacgtggtgg cgctgactgc gaaatacggt ggtttgttgt ggggcgagca cggcaaaggt
     8281 tttcgcgctg aatacagccc ggcgtttttc ggtgaggaac tttttgcaga actgcgcaaa
     8341 gtgaaagcgg catttgaccc gcataaccga ctcaacccag ggaagatttg cccgccagaa
     8401 ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc gcggtacatt cgatcggcag
     8461 atccccattg cggtacgcca gcagtggcgc ggtgcgatgg agtgtaacgg caacggttta
     8521 tgcttcaact ttgatgcccg tagtccgatg tgtccgtcga tgaagatcac ccagaaccgg
     8581 attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat ggctgcgctt gttggcggat
     8641 cgcggcgttg acccactcaa actggaacaa gaactgcctg aatccggcgt cagtttgcgg
     8701 acgttaattg cccgcacgcg caatagctgg catgcgaata aaggcgaata tgacttctca
     8761 cacgaagtca aagaggcgat gtcgggctgt ctggcctgta aagcttgttc gacccagtgc
     8821 cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc tgcagctcta tcacacccgt
     8881 tatttacgcc cgcttcgcga ccacctcgtt gctacggtcg agagctacgc accgctgatg
     8941 gcacgcgcgc cgaaaacctt taacttcttc attaaccagc cgctggtgcg caaactctcg
     9001 gaaaaacata tcggcatggt tgatttgcca ctgctgtcgg tcccctcgct acaacaacaa
     9061 atggtggggc atcgctcggc aaacatgacg ctggaacagc ttgaagctct caatgcagag
     9121 cagaaagcgc gcacggtgtt ggtggtgcag gaccccttta ccagctatta cgatgcgcaa
     9181 gtggtggcgg attttgtccg tctggttgaa aaattaggtt tccagcctgt gttactgcca
     9241 ttttcgccaa atggcaaagc ccagcatatc aaaggctttc ttaatcgttt tgcgaagacg
     9301 gcgaaaaaga cggcggattt cctcaaccgt atggctaagc ttgggatgcc aatggtgggc
     9361 gtcgatccgg cgctggtact ttgttatcgc gatgaatata aactggcgct gggcgaggaa
     9421 cgtggcgagt ttaacgtctt acttgcgaat gaatggctgg caagcgcact tgagtcacat
     9481 ccagtggcta cagtcagcgg tgaatcatgg tatttctttg gtcactgtac cgaggttacc
     9541 gctttgccgg gcgcgccagc acaatgggcc gcgatatttg cccgttttgg cgcgaaactg
     9601 gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt acggacatga agcgaaaaac
     9661 catgaaaatt cgctcgggat ctatgagtta tcctggcatc aggctatgca gcgactgccg
     9721 cgtaaccgct gtctggcgac cggatattcc tgccgtagcc aggtaaaacg ggttgaaggc
     9781 acgggggtac gccatcctgt gcaggcttta ctggagatta ttaaatgata tggaaacgga
     9841 aaatcaccct ggaagcactg aatgctatgg gtgaaggaaa catggtgggg ttcctggata
     9901 ttcgctttga acatattggt gatgacaccc ttgaagcgac aatgccagta gactcgcgga
     9961 caaagcagcc tttcgggttg ctgcatggag gagcatccgt ggtactggcc gaaagtatcg
    10021 gttccgttgc cggttattta tgtaccgaag gtgagcaaaa agtggttggt ctggaaatca
    10081 atgctaatca cgtccgctcg gcacgagaag ggcgggtgcg cggcatatgt aaaccgttgc
    10141 atctcggttc gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa gggcgtttgt
    10201 gctgttcgtc acgattgacg accgccattt tgtgacgttg tcaccagaaa agtgtgacgc
    10261 atattgcttt tggtcattat ctgcacagta aacggtggta tactgtgcag gtcttgtcta
    10321 aaagcgagga tgctatgtct actcaacttg atcccaccca gctggccatt gaatttttac
    10381 gccgtgacca gagcaatctt tcacctgccc aatatctcaa acgtctgaaa caactagaac
    10441 ttgaatttgc tgacctgctt actctctctt ctgctgaatt aaaagaagaa atctactttg
    10501 cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc aagttcgcca aatcgacctg
    10561 gattaacctc cgtccaggtt cgatgacctt cttaatactg cttatctacc gcctgatatt
    10621 gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc gggttagctt tatgagatta
    10681 ttcttatcgc cccttcaaga gctaagccac tgagagtgcc ggagataagc gccggatggg
    10741 gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt ttcttatttc tgaacgctgc
    10801 acttttttac ctatctctta aatagtcatt ttcaatacaa ctttttcttt ttcatcacag
    10861 ccttaatgct ccttagataa taaccattat ctaacaatga gatacctaat tcttagcgtg
    10921 cctgttaacc cacacatcag gggctatgct tattaaaagc aggcaaaaag catgttttac
    10981 ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat tatcactaac atgctgttat
    11041 acgctgaaag cgatgaagtg aggtaaatag atggacatgc attcaggaac ctttaaccca
    11101 caggatttcg cctggcaagg cttaacgctg acacccgcag cggcgataca catccgcgag
    11161 ctggtggcaa agcagccagg tatggtcggc gtgcgcttag gtgtgaagca aacgggctgc
    11221 gcgggctttg gctatgtgct cgacagtgtt agcgagccgg acaaagacga tctgctgttt
    11281 gaacacgacg gcgcgaagct gtttgtcccg ctgcaatcga tgccgtttat tgatggcacg
    11341 gaaatcgatt tcgttcgtga aggacttaat cagatattca aatttcacaa ccctaaagcc
    11401 cagaatgaat gtggctgtgg cgaaagcttt agggtatagg cggtactatg tctcgtaata
    11461 ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc gctgaattat aaagaaggat
    11521 tcttcaccca gttagccacc gatgagctgg caaaggggat aaacgaagag gtggtgcgcg
    11581 caatttcggc gaagcgtaat gagccggagt ggatgctgga gtttcgtcta aacgcctatc
    11641 gcgcatggct ggagatggaa gaaccgcact ggttgaaagc gcactacgac aagctgaatt
    11701 atcaggatta cagctactac tcagcaccat cgtgcggtaa ttgtgacgac acttgcgcgt
    11761 ctgaacctgg cgcggtgcag caaactggcg cgaacgcctt tttaagtaaa gaggtggagg
    11821 cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa agaggtggcg gtggatgcca
    11881 ttttcgactc tgtttcggtt gccaccactt atcgcgaaaa actggcggag cagggaatta
    11941 ttttctgttc ctttggtgag gcgatccacg atcacccgga actggtgcgt aaatatctcg
    12001 gcaccgtggt gccggggaat gacaacttct ttgcagcact taatgcggcg gtagcctctg
    12061 acggtacgtt tatttatgtg cctaaaggcg tgcgctgccc gatggaactt tccacctatt
    12121 ttcgcattaa cgcggaaaaa accgggcagt ttgagcgcac aattctggtg gccgacgaag
    12181 acagctacgt cagctacatt gaaggctgtt ccgctccggt gcgtgacagc tatcagttgc
    12241 acgcggcagt ggtggaagtc atcatccata aaaacgccga ggtgaaatat tccacggtac
    12301 aaaactggtt ccctggcgat aacaacaccg gcggtattct caacttcgtc accaagcgtg
    12361 ctttgtgcga aggcgaaaac agcaaaatgt catggacgca atcagaaacc gggtcagcga
    12421 ttacgtggaa atatcccagc tgcattttgc gcggcgataa ctccattggt gagttttact
    12481 cagtggcgct gaccagcggt catcagcaag cggataccgg caccaagatg atccacatcg
    12541 gtaaaaacac caaatcgacc attatctcga aagggatctc tgccggacat agtcagaaca
    12601 gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa tgcgcgcaat ttcactcagt
    12661 gcgactcaat gctgattggc gctaattgtg gggcgcatac cttcccgtat gtcgaatgtc
    12721 gcaataacag cgcacaactg gagcacgagg caacgacatc acgtattggt gaagatcaac
    12781 tgttttactg cctgcaacgc gggatcagcg aagaagacgc catctcgatg attgttaacg
    12841 gtttctgcaa agacgtgttc tcagaactgc cgctggaatt tgccgttgaa gcacaaaaac
    12901 tcctcgccat cagtcttgaa cacagcgtcg gataaggaat aaacatgtta agtattaaag
    12961 atttacacgt cagcgtggaa gataaagcta tcctgcgtgg attaagcctc gacgttcgtc
    13021 ccggcgaagt tcacgccatt atggggccaa acggttcggg caaaagtact ttatcggcaa
    13081 cgcttgccgg gcgcgaagat tatgaagtga cgggcggcac ggttgagttc aaaggcaaag
    13141 atttgcttgc actgtcgccg gaagatcgtg caggcgaagg catctttatg gccttccagt
    13201 atccggtgga gattccaggc gtcagtaacc agtttttcct gcaaacggca cttaatgcag
    13261 tgcgcagcta tcgcggccag gaaacgcacg accgctttga ttttcaggat ttgatggaag
    13321 agaaaatcgc tctcctgaag atgccggaag atttattaac ccgttcggta aacgttggtt
    13381 tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat ggcggtgctg gaaccggagt
    13441 tatgcattct tgatgagtcg gactccgggc tggatattga cgcattaaaa gtggtcgccg
    13501 atggcgtgaa ctcgctgcgt gatggcaagc gctcattcat cattgttacg cactaccaac
    13561 gcattctcga ctacatcaag cctgattacg ttcatgtgct atatcaggga cgaattgtga
    13621 aatccggcga tttcacgttg gtcaaacaac tggaggagca gggttatggc tggcttaccg
    13681 aacagcagta atgcgctgca acagtggcat cacttgtttg aagctgaagg gacaaaacgc
    13741 tcaccgcaag cacagcagca tttacaacaa ttgctgcgta ccggactgcc gacacgtaaa
    13801 catgaaaact ggaaatatac gccgctggaa gggctgacca atagtcagtt tgtcagcatt
    13861 gcgggagata tatccccaca gcagcgtgat gccttagcgt taacgttaga cgccgtgcgg
    13921 ctggtgtttg tcgatggacg ttacgtgccc gcgctgagcg atgcaattga aggcagcgga
    13981 tatgaagtga gcattaacga cgaccgtcag gggttacccg acgctattca ggcggaagtg
    14041 tttctgcatt tgacggaaag cctggcacaa agcgtgacgc atatcgccgt gaagcgcggt
    14101 caacggccgg caaagccatt gctgttaatg catatcaccc agggcgtggc aggtgaagag
    14161 gtgaacactg cccattaccg gcatcatctg gatctggcgg aaggtgccga agcaacggtg
    14221 atcgaacatt ttgtcagcct taatgatgct cgtcatttta ccggggcacg gttcactatc
    14281 aatgtcgcag cgaacgccca cttgcagcat atcaagctgg cgtttgaaaa cccggtcagt
    14341 caccactttg cccataacga tttgttgctg gctgacgatg ccaccgcatt tagccatagt
    14401 ttcctgctgg gtggcgcagt gttacgacac aacaccagta cgcaactcaa tggcgaaaac
    14461 agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa acgaggtgtg tgatacccgt
    14521 acctggctgg aacacaataa aggtttttgt aacagccgac agttgcacaa aactatcgtc
    14581 agcgacaaag gccgcgcggt atttaacggt ttgatcaacg tcgcgcagca cgccatcaaa
    14641 acggatggtc agatgaccaa caataatctg ctgatgggca aactggcgga agtggataca
    14701 aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca gccacggcgc gacggtgggg
    14761 cgtattgatg atgaacagat gttctatctg cgctcgcgcg ggatcaatca gcaggatgcc
    14821 cagcagatga tcatttacgc ctttgctgcc gaactgacgg aagcactgcg tgatgagggg
    14881 cttaaacagc aggtgctggc ccgaatcggt caacggctgc caggaggtgc aagatgactt
    14941 tttccgtcga caaagtgcgg gccgactttc cggtgctttc gcgtgaggta aacggtttgc
    15001 cgctggctta tctcgacagc gccgccagtg cacagaaacc gagccaggtg attgacgccg
    15061 aggccgagtt ttatcgtcat ggctacgcgg cggtgcatcg cggtattcat accttaagcg
    15121 cccaggcgac cgagaaaatg gagaacgtgc gcaagcgggc atcgctgttt attaatgccc
    15181 gttcggcgga agagctggtg ttcgtccgcg gcacgacgga agggatcaat ctggtcgcca
    15241 atagctgggg caacagcaat gtgcgggcgg gcgataacat catcatcagt cagatggagc
    15301 accacgctaa cattgtcccc tggcagatgc tttgcgcacg cgttggcgca gagctgcgtg
    15361 tgatccctct caaccccgac ggtacgctgc aactggagac gctgcctacg ctgtttgatg
    15421 agaaaactcg cctgctggca attactcatg tctccaacgt gcttggcaca gaaaatccac
    15481 tggcggaaat gatcacgctt gcgcaccagc atggcgcaaa agtgctggtg gatggcgctc
    15541 aggcggtgat gcatcatccg gtggatgttc aggcgcaggg ttgcgacttt tacgtgttct
    15601 ccgggcataa actgtatggc cccaccggaa ttggcattct ttatgtgaaa gaagccttgt
    15661 tgcaggagat gccgccgtgg gaagggggcg gttctatgat cgccaccgtc agcctgagtg
    15721 aaggcactac ctggaccaaa gcaccatggc ggtttgaagc cggtacaccc aataccgggg
    15781 gcatcattgg tcttggcgcg gcgctggagt atgtttcggc gctggggctt aatagcatag
    15841 ccgagtatga acagaatctg atgcattatg cgctatcaca gctggaatct gtaccggatc
    15901 tcactctcta tggccaacaa aacaggcttg gcgttattgc ttttaatctc ggtaaacacc
    15961 acgcctatga tgttggcagt tttctcgata attacggcat tgctgtgcgt accggacatc
    16021 actgcgctat gccattaatg gcctattaca acgtccctgc gatgtgtcgg gcgtcgctgg
    16081 ccatgtataa cacccatgaa gaagtggatc gtctggtgac cggcctgcaa cgtattcacc
    16141 gtctgctggg ataacaggga ggcactatgg ctttattgcc ggataaagaa aaattgctgc
    16201 gtaatttttt acgctgcgcc aactgggaag agaaatatct ctacattatt gagctgggcc
    16261 agcgtctgcc agaattacgc gccgaagacg gaagcccaca aaatagcatt cagggctgcc
    16321 agagtcaggt gtggattgtc atgcgccaga atgcgcaggg aattattgaa ttgcagggcg
    16381 acagcgatgc ggcgattgtg aaagggctta ttgcggtcgt ctttattctc tacgatcaga
    16441 tgacgccgca ggatattgtc aatttcgatg tgcgtccgtg gtttgaaaaa atggcgctca
    16501 cccaacatct caccccatct cgttcacaag gtctggaagc gatgattcgc gcaattcgcg
    16561 ccaaagccgc tgcacttagc taaactaaag ggacagcttt catccggttg gctcacattg
    16621 ccggatggtc aatgtttaca ctgacctgca caggagtgtt ttggtttcag gtgaacataa
    16681 gcaccatccg acgtttcagc gtgagtctcc ggcaaataca ggaggtttac aatgaaacgc
    16741 gcgtctttgc ttacactcac gcttatcggc gcttttagcg ccatccaggc tgcctgggcg
    16801 gttgattatc cgctaccacc aaccggaagc cgactggttg ggcaaaatca aacgtatacg
    16861 gtgcaagaag gggataaaaa ccttcaggct attgcccgac gttttgatac tgcggcaatg
    16921 ttgatccttg aagccaataa cactatcgcc ccggtgccaa aacctggtac gacgataact
    16981 attccttcac aactgttatt acctgatgca ccgcgtcagg ggattatcgt taaccttgca
    17041 gagctgcgcc tttattatta tccgccggga gaaaatattg tgcaggttta tccaataggt
    17101 attggattgc aggggctgga aacgccggtg atggaaacgc gtgttgggca gaaaatccct
    17161 aacccaacct ggacgcctac ggcaggcatt cgtcagcgtt cgctggagcg tggcattaaa
    17221 ttaccgccag tcgttcctgc cggaccaaat aacccgctag gacgttacgc actgcgcctc
    17281 gcgcatggta atggcgaata cctcattcat ggcaccagtg cgccggacag cgttggtttg
    17341 cgcgtcagtt cagggtgtat tcgcatgaat gcaccggata ttaaagcctt gttctccagc
    17401 gtgcggacgg gaacgccggt gaaagtgatc aacgaaccgg tgaaatattc cgtggaacct
    17461 aacgggatgc gttatgttga agtacatcga ccactatcag cagaagaaca gcaaaacgtt
    17521 cagacaatgc catacacact gccagcaggc tttacgcaat ttaaagacaa taaggctgta
    17581 gatcagaagt tagtcgataa agcgttgtat cgtcgggcag ggtatccggt ttcggtgagc
    17641 agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag agtcagcgca gaatggtgaa
    17701 ccagagcaag ggaatatgtt acgcgcgacg cagtagtggt aaatagcaga caaaaaaaat
    17761 ggcgcacaat gtgcgccatt tttcacttca caggtactat tacttgcggt atttagtagc
    17821 catgttgtcc agacgctggt tagcacgagc tgcgtcatct ttagcagcct gaacgtcgga
    17881 acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta gcgttcagag tctgaacgtc
    17941 agaagacagc tgatcgattt tagcgttgct ggagcaacct gccagcagag tagaacccag
    18001 gattaccgcg cccagtacca gtttagtagc tttcattatt aataccctct agattgagtt
    18061 aatctccatg tagcgttaca agtattacac aaagtttttt atgttgagaa tatttttttg
    18121 atgggaaggc acttattttt gatcgttcgc tcaaagaagc atcgaacgct gggttttaga
    18181 taaaaaaatt gagagaaaac aggatgcttc catcggattc atcttaaata aagtgagatt
    18241 atatagtgaa atccgatttg
//
LOCUS       DADMGC010000078        17574 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.078, whole genome shotgun sequence.
ACCESSION   DADMGC010000078 DADMGC010000000
VERSION     DADMGC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.078"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(269..1297)
                     /gene="epmB"
                     /locus_tag="JNZ73_002605"
     CDS             complement(269..1297)
                     /gene="epmB"
                     /locus_tag="JNZ73_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY8732840.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1339..1905
                     /gene="efp"
                     /locus_tag="JNZ73_002606"
     CDS             1339..1905
                     /gene="efp"
                     /locus_tag="JNZ73_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY8732841.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1957..2082
                     /gene="ecnA"
                     /locus_tag="JNZ73_002607"
     CDS             1957..2082
                     /gene="ecnA"
                     /locus_tag="JNZ73_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY8732842.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            2193..2339
                     /gene="ecnB"
                     /locus_tag="JNZ73_002608"
     CDS             2193..2339
                     /gene="ecnB"
                     /locus_tag="JNZ73_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY8732843.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2515..2744
                     /locus_tag="JNZ73_002609"
                     /pseudo
     CDS             2515..2744
                     /locus_tag="JNZ73_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(2741..3274)
                     /locus_tag="JNZ73_002610"
     CDS             complement(2741..3274)
                     /locus_tag="JNZ73_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY8732844.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3363..4496)
                     /gene="blaEC"
                     /locus_tag="JNZ73_002611"
     CDS             complement(3363..4496)
                     /gene="blaEC"
                     /locus_tag="JNZ73_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY8732845.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            complement(4559..4918)
                     /gene="frdD"
                     /locus_tag="JNZ73_002612"
     CDS             complement(4559..4918)
                     /gene="frdD"
                     /locus_tag="JNZ73_002612"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY8732846.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4929..5324)
                     /gene="frdC"
                     /locus_tag="JNZ73_002613"
     CDS             complement(4929..5324)
                     /gene="frdC"
                     /locus_tag="JNZ73_002613"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY8732847.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5335..6069)
                     /locus_tag="JNZ73_002614"
     CDS             complement(5335..6069)
                     /locus_tag="JNZ73_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY8732848.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6062..7870)
                     /gene="frdA"
                     /locus_tag="JNZ73_002615"
     CDS             complement(6062..7870)
                     /gene="frdA"
                     /locus_tag="JNZ73_002615"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY8732849.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            8195..9172
                     /gene="epmA"
                     /locus_tag="JNZ73_002616"
     CDS             8195..9172
                     /gene="epmA"
                     /locus_tag="JNZ73_002616"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY8732850.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9391..10893
                     /gene="yjeM"
                     /locus_tag="JNZ73_002617"
     CDS             9391..10893
                     /gene="yjeM"
                     /locus_tag="JNZ73_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAY8732851.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(11044..14355)
                     /gene="mscM"
                     /locus_tag="JNZ73_002618"
     CDS             complement(11044..14355)
                     /gene="mscM"
                     /locus_tag="JNZ73_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAY8732852.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSIAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(14377..15345)
                     /gene="psd"
                     /locus_tag="JNZ73_002619"
     CDS             complement(14377..15345)
                     /gene="psd"
                     /locus_tag="JNZ73_002619"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAY8732853.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            complement(15442..16494)
                     /gene="rsgA"
                     /locus_tag="JNZ73_002620"
     CDS             complement(15442..16494)
                     /gene="rsgA"
                     /locus_tag="JNZ73_002620"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAY8732854.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNIKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDG"
     gene            16589..17134
                     /gene="orn"
                     /locus_tag="JNZ73_002621"
     CDS             16589..17134
                     /gene="orn"
                     /locus_tag="JNZ73_002621"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAY8732855.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            17345..17420
                     /locus_tag="JNZ73_002622"
     tRNA            17345..17420
                     /locus_tag="JNZ73_002622"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17378..17380,aa:Gly,seq:gcc)
BASE COUNT         4071 a         4520 c         4613 g         4370 t
ORIGIN      
        1 agaaaggtta attattggtg ttagctatat aaaaagtaac aacagcaatg catatgaata
       61 attaaaggtt atgcaacgtg caaagattaa atttctcagt ttaatgacag gttgctgtac
      121 ttgcgtttgt tgctcttgcc ggatgcggcg taaacgcctt atccggccta cagttcattg
      181 caaatccatt caatttcaat gcacgatgta ggcctgataa gcgtagcgca tcaggcaatt
      241 ttaatgttta acttccctgt ttaatcagtt actgctggcg tagctggaga tccagcggcg
      301 ttttgctggg ttcgccgcca atttctcgcg ccagtttcgg caccagatat cccgacacca
      361 atgtcagcaa ctcacgcata atctgccgtg cttcgtcatc actcaccata aaatgcgccg
      421 cgccctgtac tttatctagc acatgcaggt aatacggcat cacgccggca tcgaacaacg
      481 cattactcag gtttgccaac gtttgtgcgt tatcgttcac accacgtaac agaacgctct
      541 ggttaagcag ggtgacaccg acacggcgca atttagccat cgcctgacgg aacgtttcat
      601 ctacctcatt ggcatgattg atgtgattca ccagcaagat ttgcagcgta gaacgggcaa
      661 agcgttcaac cagcgcctcg gtgatacgtg ccgggatcac aatcggcaga cggctgtgaa
      721 tccgcagacg tttgatatgc gggatggctt ccagttgtgt gagcaaccag tccagctcgt
      781 gatctttcgc catcagcgga tcgccgccgg agaaaatcat ctcgtccagt tccggatgcg
      841 cagcaacata ctcaagtgca gtctgccagt tacgcttgtt gccctgattt tcggcatagg
      901 ggaagtggcg acggaagcaa tagcggcaat ttaccgcgca gccgcctttg accagcaaaa
      961 gcgcccggtt gtggtattta tgcaacaaac caggcactac gctgtgctgt tcttccagtg
     1021 ggtcggtgga gaatccggac gcgacgacaa actcatcttg cgaggtaagt acctgacgca
     1081 aaagaggatc gtccggattg cctttctcca tgcgatcgat aaatgagcgg ggcacacgca
     1141 gggcaaacag ctttttggcg ctgcgtccgg ccaacagttt ttcgtccgcg tctatattca
     1201 aaagacgcag aagttcatca ggatcggtca caacatcggc aagttgcgtt aaccaatctt
     1261 ctctggatgg ggtatttagg gttacaatat gcgccatttt gtggcttagc taccaattaa
     1321 caaatttcag agggccttat ggcaacgtac tatagcaacg attttcgtgc tggtcttaaa
     1381 atcatgttag acggcgaacc ttacgcggtt gaagcgagtg aattcgtaaa accgggtaaa
     1441 ggccaggcat ttgctcgcgt taaactgcgt cgtctgctga ccggtactcg cgtagaaaaa
     1501 accttcaaat ctactgattc cgctgaaggc gctgatgttg tcgatatgaa cctgacttac
     1561 ctgtacaacg acggtgagtt ctggcacttc atgaacaacg aaactttcga gcagctgtct
     1621 gctgatgcaa aagcaattgg cgacaacgct aaatggctgc tggatcaggc agagtgtatc
     1681 gtaactctgt ggaatggtca gccgatctcc gttactccgc cgaacttcgt tgaactggaa
     1741 atcgttgata ccgatccggg cctgaaaggt gataccgcag gtactggcgg caaaccggct
     1801 accctgtcta ctggcgctgt ggttaaagtt ccgctgttcg tacaaatcgg cgaagtcatc
     1861 aaagtggata cccgctctgg tgaatacgtc tctcgcgtga agtaatgcgg ttgtggtgcg
     1921 gcctgcaggc tgcaccatca cttattcagg tcagagatga tgaaacgcct tatcgttctt
     1981 gttttgcttg ccagcacgct gctcacgggc tgtaacaccg cttgcggttt cggcgaagac
     2041 atcaaacatc tcggcaactc catctctcgc gctgccagct aatttttctt ctcttccgaa
     2101 aaatcatcag atttccatca tttttggcga tgttgtctat tattaatttg ctataggcaa
     2161 acataaataa cattacctaa aaggaagacg ttatggtgaa gaagacaatt gcagcgatct
     2221 tttctgttct ggtgctttca acagtattaa ctgcctgcaa caccacgcgt ggcgttggtg
     2281 aagacatttc tgatggcggt aacgcgattt ctggcgcagc aacgaaagcg cagcaataag
     2341 caataacggt acgacagcag tgtcgtgccg tttgtttttt ctgcgatcgt cataaaggta
     2401 atagttgaaa ttcccctgcc acctggcaaa atatccgttc aaccatcagc tttgcaggac
     2461 gacctgcaaa cgcctctttt caccggggac ggccccaaat ttccggagcc tgatatgtcc
     2521 tggattatct tagttattgc tggtctgctg gaagtggtat gggccgttgg cctgaaatat
     2581 acccacggct ttagccgttt gatgccgtgt ggacgggtat tggcgcagtc ggcgcggcca
     2641 tcaccggcat tgtgctgctc ggtgagtccg ctaacccgat gcgtctggcg agtctggcgt
     2701 taatcgtatt ggggattatt ggtctgaaac tcagcactca ctaactaccg ggctgctgta
     2761 cccaaataaa cttactgaca tcaaaccctt cccgggtcgc gactgccagc atctcctgtt
     2821 tcacttcgtc agaaatggtt ggcgtgcggg agagtatcca caggtagtcg cggtccgggc
     2881 cgcaaaccag cgcatggcgg tattcccgat cgagtgcaat aacgttataa ccgccataga
     2941 aaggaccaaa gaatgacact ttcagcgcag cgcgagttgg tgcgccggta aagtacgctt
     3001 tcccttcact ctgctgccac attcctctgt cagggttata gcctttatta atgacattca
     3061 ggccgccgtc atcacgcagg ctgtatgttg cggtgacttt ttccagtcca cgttcaaaac
     3121 ggtgatcaaa acgggcaatc tcataccagg taccgagata acgtttggcg tcgaaattat
     3181 tcactacggt cacgccacgc ggcggcgtag gagaactaca ggcaacgacc agaaatgcag
     3241 ctgtcgctgc ggcaacgaga gggagcaggc gcataaatgt ttccttactg gtttttttct
     3301 aagtgtagat gacagcaagg aaaagcgcag aaaaggtccg aaaattcgga cccgatgaaa
     3361 ttttactgta gagcgttgag aatctgacag gcggcggcga ctctctctgg attgggatag
     3421 tttttgttag ccagcatcac gatacccagc tctttttctg gaataaacgc gacatagcta
     3481 ccaaatccgc cggtcgcccc cgttttatgt acccatgatg cgcgtactgc aggagttggg
     3541 ggcttaatcg cttttaccgg gcgtgctgcc agtgcaattt tattgtcact gccgttaatg
     3601 atgctgtcag gattgaccgg ccagtccagc atttcccagc ccaggccctg atacatatcg
     3661 ccggtttgcc agtagcgaga ttgtgccagt tgtatccctt gttgaagcgt tttctcattg
     3721 atatcaaggg ggggtaaatt gctttgcacc cagcgggcca tatcttcaat ggtcgacttc
     3781 acaccataag cttcagcatc taacgcccct ggcgaaacat gcactgcctt accttcgcga
     3841 tatccccagg cgtaattctt ttcttctggg ggaggtacat taatccacgt atggttgagt
     3901 ttgagtggct ggaagacacg agtttgcatc gcctgctcaa aactcaaacc agacggcttc
     3961 acagccagtg cgccgaacaa accgatactg gagttggcat acagacgttg tgttcctggg
     4021 gcccatgcag gctgccagtt ttgatagaag cgcagcaagt cgcttgagga tttcacttca
     4081 tccggcacct gcaatggcag gccgccagcg gtgtaggtcg caagatgtaa tagtgtgatc
     4141 ccattccact gtttagcggt aagttcaggc cagtattttg ttgcgggatc gcttaacttg
     4201 atttcccctc gagcaatagc gtcgccacca agcacgcccg taaatgtttt gctgaccgaa
     4261 cctaactcaa acaacgtttg ctgtgtgacg ggctgctttt tggcgatgtc cgcatagccc
     4321 caagtaaagt aataaggttt accctgataa attaccgcca ccgccatacc ggggatcttt
     4381 tgttgctcta taagcggggt aattgtgcga tgcacaatat cgttgatttg ttgaggggca
     4441 gcaaatgtgg agcaagaggc ggtaattaat aaggcgcaga gcgtcgtttt gaacataagg
     4501 tctggtttcc atacaaaacg gcccgccata ggcgggccgg atttacattg gcgatgcgtt
     4561 agattgtaac gacaccaatc agcgtgacaa ctgtcaggat agcagccaga ccgtagaaaa
     4621 cccatttgcc cgcaggcacg tggattttca gatcgtgcat cgcgtggtgc atacggtgta
     4681 aaccacacca cagcggcaga acgatcatca ggaacaggaa tacgcgacca atgaagctct
     4741 gcgcgaacgc cagaacgcgc tcgtagctca gcgcatcacc cggaaacagc cccagtggca
     4801 gcagaatacc caccagcagg atcatcaccg gcgcaataat tgcgctccac ataccaccgg
     4861 ccccgaagag gccccagaat accggttcgt cagaacgctt tggatttgga ttaatcatct
     4921 caggctcctt accagtacag ggcaacaaac aggattacga tggtggcaac cacagttacc
     4981 gcccagagac ttttgataat tggctctggt cccatttttt cgtcttttac aatgatattg
     5041 gccgctttcg gtgccagttc aaaccaggtt ttggtgtgca gcagagctgc cgtcagagtg
     5101 atcaggttaa tgatcacgat aaccgggttt tgtaaaaagt cgacgaatcc cgcccaggct
     5161 tccgggccat ttttcagggc aaacagcccg aaaatcagtt caatgctgaa ccacacagcc
     5221 ggaaccgccg tgccttcgcg cagcatgtaa aagcgataaa acggcaattt tttccaccag
     5281 gtggacgtca ttggccgtac atacggttta cgtttagtcg tcatgttgca ctccttagcg
     5341 tggtttcagg gtcgcgataa gaaagtcttt cgaactttct actttgccct gctgaatggc
     5401 cgcagccgga tcgacgtgtt tcgggcagac ttcggagcaa tagcccacga aagtacagct
     5461 ccatacaccg ttctggctgt tcaactgcgc catacgctcc ttcttaccgt ggtcgcggct
     5521 atcttcgtta taacgatgcg ccagcgtaat ggcagccgga ccgatgaact ctgggttcag
     5581 gccaaactgc gggcacgcgg cgtagcacag accacagttg atgcaaccgg agaactggtg
     5641 atactttgcc atctgcgccg gggtctggat gttagtaccc tgatccgcgg tgcgggagtt
     5701 gccgatgatg tacggtttga tcgcttccag actttcgatg aagtgggtca tatcgaccac
     5761 cagatcgcgt tcaatcggga agttagctaa cgcttcaacc ttcataccgt cggtgtaatc
     5821 acgcaggaag gttttacatg ccagttttgg cacgttgtta accatcatgc cgcaggaacc
     5881 acaaatcgcc atacggcagg accagcggta actcaggtcc ggtgccaggt tgtctttgat
     5941 gtagcccagc gcatccagta atgaggtagt tgcgtcataa ggcacttcat agaatgcgct
     6001 atgcggtgcg gtatcgactt ccgggttata gcgcaccacc tcaattttca ggtttttcat
     6061 ctcagccatt cgccttctcc ttattattgg ctgcttccgc cttatcggct gcatccgctt
     6121 cgccaccgta aacgcgttta gctggcggca gcgtagtaat cttcacgtcg ctgtactcca
     6181 ggcgagtcgt gccatcagca tcgcggaagg cgagggtgtg tttgagaaag ttgacgtcgt
     6241 cacgctcggt gcaaccttcg tccagacgct ggtgtgcgcc gcgggactct ttacgtgcca
     6301 ttgcggagtg cgccatacat tcagcaacgt tcagaccgtg gctcagttca atggtgtaga
     6361 gcaggtcggt gttgaacacg ctggaggtgt cggtgatgcg cacgcgcttg aagcgttcct
     6421 gcagctctgc cagcttgtcg atggttttct gcatcagttc cggcgtacgg tagataccgc
     6481 aaccttcttc cattgccagg cccatttcgt cgcggatctt cgcccagttt tcgccgccat
     6541 cctggttaac cagatctttc agacgttgtt caacgccagc tgcctgcgct tcaattgccg
     6601 cttcgttgcc attaccggca gttgctgcac gctctgtcgc ttgttcaccg gccagacggc
     6661 cgaagaccac cagttccgcc agggagttgg agcccagacg gtttgcaccg tgcagaccaa
     6721 cagaggaaca ttcacccacg gcgaacagac ctttaatgcg ggtttcacag ttctgatcgg
     6781 tttcgatacc gcccatggtg tagtgtgcag tcggacgtac cggaatcggt tctttaaccg
     6841 gatcgacgcc aacgtacgct ttcgccagtt cgcagatgaa cggcagacgt tcatgcagtt
     6901 ttttctcgcc gaggtgacgc aggtcgagat aaaccacatc accacgcggc gtggagatgg
     6961 tgttgccttt acgccattcg tgccagaagg cctgagaaac tttgtcgcgt ggacccagtt
     7021 ccatatattt gtttttcggc tcgcccagcg gagtttccgg gcccatgccg taatcttgca
     7081 gataacggta gccatttttg ttgaccagaa taccgccttc accacggcag ccttcggtca
     7141 tcaggatacc ggaacctggc agaccggttg ggtgatactg aacgaattcc atgtcacgca
     7201 gcggaacgcc gtggcttagc gccataccca taccgtcacc ggtaacgatg ccgccgttgg
     7261 tgttgtaacg ataaacgcga cccgcaccgc cggtagccat aacgaccgcg ttagcacgga
     7321 tctgcaccaa cgtgccttcc atcatgttca ttgctaccag gccgcgaaca tgaccatcat
     7381 caaccagaat atccagcacg aaatgttcgt caaaacgctg gatctgcggg aattgcagag
     7441 aggtctggaa cagcgtgtgc agcatatgga agccggtctt atcggcggcg aaccaggtgc
     7501 gctcgatttt catgccgccg aagcgacgta cgttgacgct accatccggg cgacggctcc
     7561 atgggcatcc ccacagttcc agttgggtca tttcggttgg gcagtggtgg acgaaataat
     7621 ccacgacatc ctgctcacac aaccagtcgc cacccgctac tgtatcgtga aagtgatatt
     7681 cgaagctgtc atgatcctgc gcgacagcgg cggagccccc ttctgcagca acggtatggc
     7741 tacgcatcgg gtatactttt gagattagtg cgatttttgc atttggattt gcctgcgcgg
     7801 cagcaattgc agcacgtaat cccgcgccac cggcgcctac aatggcaaga tcggcttgaa
     7861 aggtttgcac gacattcctc cagattgttt ttatcccaca gccccgtact tcagggtaag
     7921 tgcctgaaag ttacggtctg cgaacgctat tccactgctc ctttataggt acaacagtat
     7981 agtctgatgg ataagtctga aatttgacga gatcgatttt tttagtgcgc aagagggcta
     8041 aattatcact gaagatgatt aatttaatta ctaaaccatc agatcgtgct ttttttagtc
     8101 actgcccacc gctgtttgat tcctgcgtaa aatttgtctc gccgtcgcgt tgcgagtaga
     8161 cttcgtgccc ttgtcaaaaa ctggagattt aactatgagc gaaacggcat cctggcagcc
     8221 gagcgcatcc attcctaact tattaaaacg cgcggcgatt atggcggaga tccgtcgttt
     8281 ctttgccgat cgtggagtgc tggaggtgga gacgccttgt atgagccagg cgacggtaac
     8341 cgatattcat ttggtcccgt ttgagacacg tttcgttggc cccgggcatt cgcaggggat
     8401 gaatctctgg ttaatgacca gcccggaata ccatatgaaa cgcctgctgg ttgctggttg
     8461 tgggccggta ttccagctgt gccgcagctt ccgtaatgaa gagatggggc gttatcacaa
     8521 ccctgagttc actatgctgg agtggtatcg accgcactat gatatgtacc ggttgatgaa
     8581 cgaggtggac gatctcttac aacaggtgct ggactgcccg gcagcagaaa gcctttctta
     8641 tcaacaagct ttcttgcgtt atctggaaat tgacccgctc tctgccgaca aaacgcaact
     8701 gcgggaagtc gcagcgaaac tggatttgag caatgttgct gataccgaag aagaccgcga
     8761 cacgttgcta caattgctgt ttacctttgg cgtagagcca aatattggta aagaaaaacc
     8821 gacctttgtg taccactttc cagccagcca ggcatcactg gcgcaaatca gtaccgaaga
     8881 tcatcgggtc gctgaacgct ttgaggttta ttataaaggt attgagctgg cgaatggttt
     8941 ccatgaatta acggatgccc gtgagcagca acaacgcttt gaacaagata accgtaagcg
     9001 cgcggcgcgc ggtttgccgc agcaccccat tgaccagaat ctgattgaag ccttgaaagt
     9061 cggtatgcct gactgctccg gcgtggcatt aggtgttgat cgtctggtga tgttggcgct
     9121 gggcgcggag acactggctg aagtcatcgc ctttagcgtt gaccgggcat aattctgaaa
     9181 aattactgaa ttaacagcga agaatggcgt gataagcgcc attctgtagc atatgttttc
     9241 atttttccgt taaaggccac aatttaagac tggatatttg atatcatcaa ggtatcaatt
     9301 ctgtattggt ttttacgctg cctgctcaat gttgcgcagt gcagttcagt gggcacgtgt
     9361 tatacacgcg ctgaaatgaa ggatggtttc atgcctcaca cgataaaaaa gatgagtctg
     9421 ataggactca tattgatgat ctttacttcc gtatttggat ttgccaatag cccatcggct
     9481 tattacttaa tgggttatag tgcgattccc ttttatatat tttctgcatt gttattcttt
     9541 attccattcg ccttaatgat ggctgaaatg ggagctgctt atcgcaaaga agaaggtggt
     9601 atctattcct ggatgaataa tagtgtcgga ccacgttttg ccttcattgg tacgtttatg
     9661 tggttttcct cttatatcat ctggatggtg agtacctccg cgaaagtttg ggtaccgttc
     9721 tcaacattcc tctatggtag tgacatgacc cagcactggc gtattgccgg actggagcct
     9781 acgcaggtgg ttggtctgct ggcagtagca tggatgattc tggtcaccgt cgttgcttca
     9841 aaggggatta ataaaattgc ccgcattact gcggtgggcg gtattgcagt aatgtgtctg
     9901 aatttagtat tgctgttagt aagcattact attttgttat taaatggtgg gcatttcgcg
     9961 caggatatta atttccttgc atcaccgaac ccgggttatc agtccggtct ggcaatgcta
    10021 tcgtttgtgg tatttgctat ttttgcctat ggcggaattg aagcggttgg tggtctggtc
    10081 gataaaacgg aaaatccaga aaagaacttt gccaaaggta ttgtttttgc cgctattgtt
    10141 atttcaatcg gttattcgct ggcaatattt ttatggggcg tcagcacaaa ctggcagcag
    10201 gtattaagta atggttccgt taaccttggc aatattacct atgtgctgat gaagagcctc
    10261 ggggtgacgc tgggtaacgc actgcatttg tcacctgaag cgtcattgtc gctgggcgta
    10321 tggtttgcgc gtattaccgg actttcgatg ttcctcgctt ataccggtgc gttctttacg
    10381 ctttgctatt caccgctgaa agccatcatc caggggacgc cgaaagcgtt gtggccggaa
    10441 ccgatgacgc gcctgaatgc gatggggatg ccgtctatcg ccatgtggat gcagtgcggg
    10501 ttggttactg tcttcattct gctggtttcg tttggtggcg gtaccgcatc ggcgttcttt
    10561 aacaagctga cgctgatggc gaacgtgtct atgacgcttc cttacctgtt cctcgcgctg
    10621 gctttcccgt tttttaaagc acgtcaggat ctcgacagac catttgtgat tttcaaaacg
    10681 cgtatgtcgg caatgattgc gacggtggtt gtcgttctgg tggtgacatt tgcgaacgtc
    10741 ttcaccatta ttcagcctgt ggttgaagcc ggagactggg acagcacatt gtggatgatt
    10801 ggcggccctg tcttcttctc gctgttagcg atggcgattt accagaacta ttgcagtcgc
    10861 atggcgaata aacctgagtt agctctcgac tgatcatatg aaatcctgca actcatcgtc
    10921 tctccggcga tgagttgtca ggaagtaatg cgtatggatg atactctcca gggccggtga
    10981 taactgaaga tgatgttaag ctgctaaatg agccacgttg acatcatttg gcagcttcat
    11041 gcattacaaa cttcccgcct gacgaccttt gcccgcagac gtcagcgttc tccccgtttg
    11101 tttaccgttg aggctttcca gacgcatctg gaagggcggg aatggcatat cgataccgtg
    11161 ggcgtggaag cctgccagaa tcagctggtg gatctcatgg cgtagcggca tacggtgacc
    11221 catctcagcg gcgtaaatac gcagctcgaa aatctgaatc ccctgttgca gatccaccag
    11281 gaagacttcc ggtgccgggt tgtcgatcac cagcgagcag cgacgcgctg cggtgagcag
    11341 gatttccgtc acttcttcac tgttggcatc ggcaggggcc ggaatcgtca acactacgcg
    11401 cgtgaccgag tcagagagcg accagttgat aaactgctcg gtaataaacg ccttgttcgg
    11461 cacgattatc tctttacggt cccagtcgct gatggtggtg gcgcgggtgt taatttttgt
    11521 cacgctacca gtaagatcgc ggatcgttac cgtatcgcca atgcgaatcg gtttttcgaa
    11581 caggatgatc aggccagaga taaagttggc gaaaatttcc tgcaaaccaa aaccgagacc
    11641 aacaccgagc gcggcaacca gccactgcaa tttcgaccac tcaataccaa tcattgagaa
    11701 gccgaccagc ccgccaatca gcatcagcag atattttgtg atggtggtga tggcgtaacc
    11761 cgtacccggc gttaaatcca ggtgctgcaa aatcgccagt tccagcagcg cgggcaggtt
    11821 gcgcaccagc tgcgtggtga tgataaatac cagaatggca atcagcaccg caccgagggt
    11881 aattggctcc agactttcta cgccctgtac cgtggaggtg acatcccaca gcgaaatatt
    11941 ttcgaggaag ccgaaagcgg aatggatttc tgaccacagc acaatgaccg aaagcagggc
    12001 gatcaacatc aaaattgagc gcaccagccg caaggattgc gcactgatgg catcgagatc
    12061 gacttcgctt tcatcgactt caattgctcc ttccgggcta ctgtgatgat gcgcttcctc
    12121 ttcgccacgc gcacgttgcg ctaacatctc tgcccggcga tgcttcgccc ggtcaaacgc
    12181 cagcctgcga cgctggatca gcatccagcg gcggataacg tgataaacca ccagtagcag
    12241 gaaccagatg gcaatcgagg tttcaagcct cgctaacagt gcctgcgccg ttgccagata
    12301 acccaccgcc gacgccagaa tggcaaccag tggtgcgcca atcatcatgt tccacagcat
    12361 atggttggta atgttgtcgc cgctgccttc tttgttgaga tacaacggaa taccggcttt
    12421 tttcaggctt agggtgacca ccgccagcgc accgcaaatg aggataaagc aaagccgtcc
    12481 cagcgaaccg gaaaattcgc ggtcgtcgag gttatcgaac atcatcagcg ccataatcag
    12541 cccgatgctc atcaggtagt agcgcatccc acgggaaaca cgttcgcgcg gccagccaaa
    12601 atgagcgata aacaagccgt tcgggcgggc aaaggtggcg caaatcatca ctacccacag
    12661 cagcggcacg gtggccgtta caccatcgcc aatcgcgacc gccagcggat aaggccacgc
    12721 ctcgcgcaag ccgtaaccca gcgtcatcca cagcaccggt aacggtgacg cgacgagaat
    12781 cgaccagaaa agagtgcgca acgtcagcca gaagtgatcc tgagtcactt tgccgacttt
    12841 cgccgccgaa cgttcaagaa aacgggtgaa atagcggcgc gagtaaatac tgcaaccgac
    12901 cagaatcaac gcgccaaaca gcggcaaaat tgtctctttg ctggtcagca tcatcacact
    12961 ggctttgccc aactgactga aggtgtccag cgaaatgaga cgacgcagat cctgggcgat
    13021 ttccagcggc caggcgatgg tcatcgggcg cacgtcagag gtccagaaca gatagcggtg
    13081 cgttgcttcg ttcacctctt tcagcgcatc ctccagttgc ccgttggaga ctttcagctt
    13141 ggtcagttcc aatagtagcg tgtcgccacc ctgcaacaat gagttcagca actcacgctg
    13201 agtgcgcagt tgtgcttcca gaatacggtt ttgctcggcg gtcagcggct gaccgtcggc
    13261 ctgatggatt tgccgtagca gcggctgttt attaagtaaa tcctcataac gtaaccgttg
    13321 cacacgcaac tgcgccattt cggtatcaag ctgttgtggt tttggcattt ctggtagccg
    13381 tgccacctgc gcccgcagcg cttcgccgag caggttggac gatcccagcc attgcgactg
    13441 ttcacgcagc gtattcaacg cctgccggac ctgtaacgtc tggctggcag cctgacgctg
    13501 ttgcgaggca acgagctcca tccgctgcgc ctgttgattc agcgctgccg atagttcgcg
    13561 gttaattttg aattgcgcga cgatatcttt cggcaaatcg gcactgtttt ctgccagtaa
    13621 ttcggtactt tccagcgccc gctccgcctc aagctgacgt tggctattca actgattacg
    13681 taagccctgc aaatacgcat ccagttgctg gctctctttt tccgccagct ctgagcgtaa
    13741 gcgcgctaat tcctggcggt tattggcaga cagctgcgcc agctccagtt catcaacgag
    13801 cgccttaaga cgtgcagagt cagactgcaa cgcgaaattt tgtgcctgat tgagcggagt
    13861 attgccggta agcgttccca ggcggcgctc gatctcattt aactgacggc gggcgtcggt
    13921 ttgctgttgc ggcagttgat tcagcgaatc ggcaatctcg cgggcgcgct cctgctcttg
    13981 ctgggcctga cggcttttat ccagcaactg gctgctgacc tggagaattt cctgattcag
    14041 cgcgtcggta gacattcccg gcgacacgct gcgcggctcg tcacgcatgt tgtttaattg
    14101 tgcgcgcaga gtagcggaga gtttcggata attatcgata acttgctgat attgtttgat
    14161 gcgctcaagg gaaccttttc gttcctcaag cgcatttaag gcagactgga gcgcctctac
    14221 gacttccggc tgtgcgggtt tcgccgcttt tgcctgctcc agttcctgag tgatttgttt
    14281 gctatcgggg gccgtcgcgg cgtacgcccc ccaactgagg caccaggcca tcagaaaagt
    14341 gataatcagg cgcacgtcag cgtttccttt gatggattag acctggtctt ttttatcgtc
    14401 aaccaatggc ctggcgtcgt gttctgcttc gatctcttca gcaggaagtg gggcgggttc
    14461 agcgtctggc gtaacaaagg tttcggtaga tactgccagc ggctggccaa ttttcgtgac
    14521 agacaggctt tccagttgct caaccagatt cactttaccc ggtgcaaaca ggttgataac
    14581 ggtggaaccg agtttaaagc gacccatttc ctggcctttc agcagtgcca cagaaccgtc
    14641 gttttccccg gcaggccagg tccagcgctt gatgatacct tcgcgcggcg gcgtaatggt
    14701 gcccgcccag accgtctcaa tgctgccaac aatcgtcgct ccgaccagaa tctgcgccat
    14761 tgggccaaat tcggtatcga aaaggcaaat cacgcgttcg ttacgggcaa acagattcgg
    14821 cacgttctga gcggtgagat ggttaacgga aaagagatcg cccggcacgt agatcatctc
    14881 acgcagaata ccgttgcacg gcatgtgtac gcgatggtag tcacgcgggg agaggtaagt
    14941 ggtcacaaac gtaccgttgc ggaacaggtc cgccatcaga tagttgcctg ccagcagggc
    15001 ttcgaggctg tagttgtggc ctttggcttg caggatttta tcttcttcga ttttacccaa
    15061 ctggctgata acgccatcgg caggcatgac cagtacattc ggatcggtat cgattgggcg
    15121 tacttcgtca cgcagcggac ggacaaagaa ttcgttaaag gtgcggtagc tggcggtgtc
    15181 cggcttttgc gcctctttca tgtcgacctt gtagtattta acgaacagat cgataaccag
    15241 ttttgtcagc catcctgccc gcttgcttgc gccccaaccc gccaggcgag taagccatag
    15301 tttcggcaga atgtactgta gcgaaagttt aaatgaattt aacaaggtag cctccaggcc
    15361 attgttttgt cgttcctgat ccggccaaca tgccggatcc tgaaaaaaag gggacgattc
    15421 taacgacggt tagcttaatt gtcagccatc cgtatcagaa aagtttttac gcgtttttac
    15481 ctgcgccatg ctttccagaa tacggtgata gttttcgaaa cgggtttccg cgattttccc
    15541 ttcctcaacc gcttcccgga tagcgcagcc cggatcggta tcgtgtttgc aatcgcgata
    15601 tttacacaga cctaaatagt catggaattc gacaaagccc tgagtgattt gttccggctc
    15661 caggtgccag aggccgaact cacgcacacc aggggaatca atcacatcac cgccgtgcgg
    15721 gaagtgatac agccgagcgg cggtcgtggt gtgctgaccg agccccgagt tgtcagagac
    15781 atcgttggtc aggatctctt tttgcagccc cagcaacgcg ttcagcaggc tggatttacc
    15841 gacgccagac tgcccggcaa aaatgctgat gcgcccggtc aacgcctctt ccagcggttt
    15901 cagcccatcc tgagtatggc tggaaaccat caatacgcga taaccgatgt tgcggtagat
    15961 atccatctgc tcgttgacga acgccatgcc ttcgtcgtcc agcaggtcta tcttgttgag
    16021 cacgataatc ggctcaatct gcaaggtttc gcaggccacc aggtaacggt cgataatatt
    16081 gagcgacagc tccggcaaaa tggcggagac aatgacaatc tggtcgatgt tggcggctat
    16141 gggtttcacg ccgtcgtaga aatccggacg cgttaacacc gaggtgcgct catgcaccgc
    16201 ttccacgatc cctttgatat ttacgccttc cgccgccggt ttacccggac gccagactac
    16261 gcggtcgccg gttaccagcg aacggatggt acggcgaata ttgcagcggt gaacgtcgcc
    16321 atcggcggat tccacatcag cgtgcatacc aaagcggctg atgacgatac cttcatcagg
    16381 ctcgccaaac agattgtcgt cgtagtcggg cttctcctta gacgttttaa gacgacgctg
    16441 gtgattggcg ttcacgcggc gctgctggcc tttggagagt ttatttttac tcaatcgtac
    16501 tgactcctgg tcgcccctga tgggcaaaac atctatgata cacgcaattg tggatcaata
    16561 tagtcactgt gaatgggtgg aaaatagcat gagtgccaat gaaaacaacc tgatttggat
    16621 cgatcttgag atgaccggtc tggatcccga gcgcgatcgc attattgaga ttgccacgct
    16681 ggtgaccgat gccaatctga atattctggc agaagggccg accattgcag tacatcagtc
    16741 cgatgaacag ctggcgctga tggatgactg gaacgtgcgc acccataccg ccagcgggct
    16801 ggtagagcgc gtgaaagcga gcacgatggg cgatcgggaa gctgaactgg caacgctcga
    16861 attcttaaaa cagtgggtgc ctgcgggaaa atcgccgatt tgcggtaaca gcatcggaca
    16921 ggaccgtcgt ttcctgttta aatacatgcc ggagctggaa gtctacttcc actaccgtta
    16981 tctcgatgtc agcaccctga aagagctggc gcgccgctgg aagccggaaa ttctggatgg
    17041 ttttaccaag caggggacgc atcaggcgat ggatgatatc cgtgaatcgg tggcggagct
    17101 ggcttactac cgcgagcatt ttatcaagct gtaaaatttt tcctggtcac gtaagcgccg
    17161 gaatccactt gcggcggcgt gattttgacg ctaaatcgaa cgtcttgctg ataatttcag
    17221 cacttgagat aaaaacgcaa aaaaaacttt tttggggggt tgcagaggga aagatttctc
    17281 gtataatgcg cctcccgtaa cgacgcagaa atgcgaaaat tacgaaagca aaattaagta
    17341 gtatgcggga atagctcagt tggtagagca cgaccttgcc aaggtcgggg tcgcgagttc
    17401 gagtctcgtt tcccgctcca aaatttgaaa agtgctgcaa agcacagacc acccaatcgc
    17461 agtatttcga attgcgacaa ggcggcaact gaacgaatcc ttgggaactt acaaaagtat
    17521 gtgactaagg tgagtgaagg cagccaaggc agtagcggtt cgaaagacga agat
//
LOCUS       DADMGC010000079        17458 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.079, whole genome shotgun sequence.
ACCESSION   DADMGC010000079 DADMGC010000000
VERSION     DADMGC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.079"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            564..1334
                     /locus_tag="JNZ73_002623"
     CDS             564..1334
                     /locus_tag="JNZ73_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HAY8732856.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="JNZ73_002624"
     CDS             1325..1798
                     /locus_tag="JNZ73_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAY8732857.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="JNZ73_002625"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="JNZ73_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HAY8732858.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPVSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="JNZ73_002626"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="JNZ73_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAY8732859.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGVIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="JNZ73_002627"
     CDS             3233..3727
                     /locus_tag="JNZ73_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HAY8732860.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="JNZ73_002628"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="JNZ73_002628"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HAY8732861.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /locus_tag="JNZ73_002629"
     CDS             complement(6410..7432)
                     /locus_tag="JNZ73_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAY8732862.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7571..8485
                     /locus_tag="JNZ73_002630"
     CDS             7571..8485
                     /locus_tag="JNZ73_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY8732863.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGILTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8699..10060
                     /locus_tag="JNZ73_002631"
     CDS             8699..10060
                     /locus_tag="JNZ73_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8732864.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="JNZ73_002632"
     CDS             complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="JNZ73_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY8732865.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="JNZ73_002633"
     CDS             complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="JNZ73_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HAY8732866.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12610..13899
                     /locus_tag="JNZ73_002634"
     CDS             12610..13899
                     /locus_tag="JNZ73_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HAY8732867.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPQVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13896..15362
                     /gene="hpaE"
                     /locus_tag="JNZ73_002635"
     CDS             13896..15362
                     /gene="hpaE"
                     /locus_tag="JNZ73_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HAY8732868.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTANRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATERNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVDDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKLSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLHQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15364..16215
                     /gene="hpaD"
                     /locus_tag="JNZ73_002636"
     CDS             15364..16215
                     /gene="hpaD"
                     /locus_tag="JNZ73_002636"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HAY8732869.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16225..16605
                     /locus_tag="JNZ73_002637"
     CDS             16225..16605
                     /locus_tag="JNZ73_002637"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HAY8732870.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16673..>17458
                     /gene="hpaH"
                     /locus_tag="JNZ73_002638"
     CDS             16673..>17458
                     /gene="hpaH"
                     /locus_tag="JNZ73_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HAY8732871.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGHPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSI"
BASE COUNT         4294 a         4627 c         4374 g         4163 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tggggtctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccggtaagcg
     2101 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggtg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaggcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact aaatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgcccg ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc tgacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cccaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tctccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcat
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
     8281 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     8341 tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8401 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8461 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8521 acggttttat caaaagataa ataccggaaa ttcccgctta cctatgctca caatcagtcg
     8581 actaaaaatg tttttgtgag cggtaataaa gtcctaaaac tttaacctgc cgttaatgtc
     8641 aaccttgcgt aatgcacaat tacgtaaaca attaaaatta taaatacagg agttcggcgt
     8701 ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt cgtcggcaga
     8761 tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac gcattcaaac
     8821 caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc gcagttcgct
     8881 gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg aaatcggcgc
     8941 tttgctctcc gtgttttcac tcgcttacgg cattgcgcaa cttccttgcg gcccactatt
     9001 ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt cactgttcca
     9061 ggccatgtct ggcatggtgc acagctttac gcagttcgtg ttggtgcgta tcggtatggg
     9121 gattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact ggttcaacat
     9181 caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg gcgttgcggt
     9241 aagcccaccg attctggcgg caatgatgct ggtgatgggc tggcgcggga tgtttattac
     9301 cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc gcaaccgcga
     9361 gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg
     9421 ccgagatccg ctcaattttg ccgaatggcg cagcctgttc cgtaaccgca caatgtgggg
     9481 aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg cctggcttcc
     9541 tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga tggcggctat
     9601 ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg actggctggt
     9661 caaaggggga atggctccga ttaaaagccg taagatctgc attattaccg ggatgttctg
     9721 ttctgccgcc tttacgctga tagtaccaca agcgacaaca tccatgatgg cggttctgct
     9781 gattggcatg gcactgttct gtattcactt tgccggaaca tcctgctggg gcttgatcca
     9841 cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact ttgccagctt
     9901 catctgcgcc tctttcgcgc cgatcattac tggttttatt gttgatacaa cccactcatt
     9961 ccgtctggca ctaatcattt gcggttgcgt caccgcagcg ggagcactgg cgtacatctt
    10021 cctggttcgc cagccgatca acgacccgcg taaagattaa actccctatg cccgataaca
    10081 ttttgcttat cgggcatttt tatggcgatt aaaacgtttc ccagttctcc ccgctatctg
    10141 ccactgccat tttacgcggc gtagctggcg tcacggtttt taccacagcc gatgtttcac
    10201 gctgctgttg ctgctgttgc tgaatccgga acactgccac agcttcggtc aggcgactgg
    10261 cctgctcttc cagcgcggcg gcggcagcgg cagattcttc caccagcgcg gcgttctgtt
    10321 gagttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct
    10381 catcagaagc agaagctatt tcgcccataa tgtccgtcac gcgggtcaca gcactgacaa
    10441 tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca
    10501 ccgagtcttc aatcaggctt ttaatttcac gagccgcctg ggcgctacgc tgggccagat
    10561 tacgcacttc tcccgcaacc accgcaaaac cacgtccttg ctcacccgca cgcgctgctt
    10621 caaccgccgc gttcaaagcc agaatattgg tctggaaggt aatgccgtca attacgctga
    10681 taatatcggc gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat
    10741 ctaccacttt gccgccgcgt tgcgccgttt cagaagcact taatgccaga tggctggcct
    10801 ggcgcgcatt ctcggcgttc tgtttcaccg ttgcggtcag ttgctccatg ctggctgccg
    10861 tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcatta ttgccggtag
    10921 cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac
    10981 gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc
    11041 catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca
    11101 ggcgattcat tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg
    11161 ccagtacgac gatcatcacg cccaccagaa tccacatcgc ctggctgtag gaggcattgt
    11221 tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct
    11281 tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg
    11341 cacctaacag ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga
    11401 tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc
    11461 agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg
    11521 aaccgctacc aatattattc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt
    11581 tacgcgtctg caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg
    11641 tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaaaaac agaccgcctg
    11701 atgtcagttg taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga
    11761 tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc
    11821 aaaaacttta tgaaaaggtg taacccgcca tccatggaaa aaccaggcgc tggttatcag
    11881 atactaaaaa gttattcatt atcgcccggg atgtcttctt gtcgggaatt gcccagagca
    11941 ataaattcct ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca
    12001 atctgccgat aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct
    12061 ttggtcagcg agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa
    12121 ccgtcgcgtt ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga
    12181 tacgccagat cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac
    12241 tgttgctcag tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct
    12301 cgcgcctgga gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca
    12361 aaatcattaa tatagaaaca attaatcatc acgttaccgg gactgagctg cccccgcaat
    12421 tcttcatttt atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta
    12481 tctttaaatg taaatagttt gttaattaga tcacatttat atcacttact tttgccaaac
    12541 tacgcaatcg ggaataaaag taatcattaa catattaatg attaagatga tcgaatccga
    12601 ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat
    12661 gcctggcagg aagcgttcca gcaatctccc tacaaagccc cgcctaaaac tgcggtctgg
    12721 tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt
    12781 gaaaaagtac tgagcggtgc gactgttgcg ctgattgtgg ggaaaacggc gacgaaagta
    12841 cgtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg
    12901 ccggaagaga gcttttaccg tccggcaatc aaagcaaaat gtcgtgatgg attctgcccc
    12961 attggcgaaa ccgtggctct cagcaatgtc gataatctga ctatctatac cgagatcaac
    13021 gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc acagctgctg
    13081 agcgccctga gcgaatttgc cacgctgaat ccaggcgatg ctattctgct cggcacgcca
    13141 caggcgcgcg tggaaataca gccaagcgat cgcgtgcgtg ttctcgcaga aggtttcccg
    13201 ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcgcaagag cttcccaacg
    13261 ctgccacacc cgcacggtac gctgtttgcc ctcggcctga actacgccga ccacgccagc
    13321 gaactggaat ttaagccacc ggaagagccg caggtgttcc tgaaagcgcc gaataccctc
    13381 actggcgaca accagacctc cgtgcgcccg aacaatattg aatacatgca ctacgaagcg
    13441 gagctggtgg tagttattgg caagcaggcg cgtaacgtca gcgaagccga tgccatggat
    13501 tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gcgactatct ggaaaactac
    13561 taccgcccta acctgcgggt caaaagccgc gacggactga cgccgatgct ttcaaccatc
    13621 gtgccgaaag aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc
    13681 gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc
    13741 tacttaagcg aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacaccaaaa
    13801 ggcttatctg acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc
    13861 ctggtgaacc gaattgtgag tgaggaaaca gcgaaatgaa aaaagtaaat cattggatca
    13921 acggcaaaaa tgttgcaggt aacgactact tccagactac caatccggca acgggtgaag
    13981 tgctggcgga tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga
    14041 aagaggcgtt cccgaaatgg gctgatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc
    14101 gtctgggcga tctgatcgac cagaacgtgc ctgagatcgc cgcgatggaa accgcggaca
    14161 cgggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttct cacaactttg
    14221 aatttttcgc ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga
    14281 tgctcaacta cacgctggtg caaccggtag gcgtttgtgc gctggtgtcg ccgtggaacg
    14341 tgccgtttat gaccgccacc tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg
    14401 tgctgaagat gtcggaactc tccccgctga ccgccaaccg cttgggtgag ctggcgctgg
    14461 aagctggtat tccggcgggc gtgctaaacg tggtacaggg ctacggcgca accgcaggcg
    14521 atgcgctggt ccgtcatcat gacgtgcgtg ccgtgtcgtt caccggcggt accgccaccg
    14581 agcgcaacat catgaaaaac gccgggctga aaaaatactc catggagctg ggcggtaaat
    14641 caccggtgct gatttttgaa gatgccgata ttgagcgtgc gctggacgcc gccctgttca
    14701 ccatcttctc aatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcaacaga
    14761 gcatctatcc agaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtggacg
    14821 atccgaacga tccgaatacc caggttaggg cgcttatcag tcagcaacac tgggaaaaag
    14881 tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgttggcg ggcggcccgg
    14941 ataaactgtc cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaacggtgc
    15001 tggcggacgt agataaccgt atgcgcgttg cccaggaaga gattttcggg ccggtcgcct
    15061 gcctgctgcc gtttaaagac gaagccgaag gcttacgcct ggcaaacgac gtggagtacg
    15121 gcctcgcgtc gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca
    15181 ttgaagcagg catggtgttc gtcaacaccc agaacgtgcg cgacctgcac cagccatttg
    15241 gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg
    15301 cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct
    15361 gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatctctct
    15421 gaactaccag gaaaaaacca cggttgtcgc cagggtgcga tcgacgggca taaagagatc
    15481 agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgatac tcactggctg
    15541 gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac
    15601 gagctgccgc attttattcg cgacatgacc tacaactacg agggcaaccc ggagttgggg
    15661 cagctaattg ccgaggaagc cttaaagctc ggcgtgcggg caaaagcgca caatattccc
    15721 agcctgaaac tggaatacgg cacgctggta ccgatgcgct acatgaatga agacaagcac
    15781 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag
    15841 ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc
    15901 agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc
    15961 tacacccgcg agttcgaccg ccagatggac gagcgtgtgg tgaagctgtg gcgcgaaggc
    16021 cagttcaaag agttttgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc
    16081 aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag
    16141 gtggagttta ttaccgagct gttcccaagc tctggcaccg gtcaggttaa cgctgttttc
    16201 ccgctgcccg cgtaaggacg ttttatgccg cactttatcg tggaatgtac tgaaaatatt
    16261 cgtgaagaag cccgcttacc tgagctgttt gccagtgtta acactgcact ggcggcaacg
    16321 gggatttttc cgcttggggg aatacgtagc cgggcgcact ggatcgatac ctggcaaatg
    16381 gctgatggca agcacgatta cgcctttgtg catatgacgc taaaaatcgg cagcggacgc
    16441 agtctggaaa gccgtcagga agtgggcgaa atgctgtttg atctgattaa aacgcacttc
    16501 gcctcactga tggagagccg ttacctagcg ctttcatttg agattgcaga gctgcacccg
    16561 acgttgaatt tcaaacagaa caacgtacac gcgttgttta aataaatttg caccagcttc
    16621 caggccggat gataaggcgc tgccgcatcc ggcaatgcca caggatatcg ctatgttcga
    16681 taaacacacc cacaccctga tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca
    16741 gatccgcgcg atctcgctgg attacccgga gatcaccatc gaagacgcct acgccgtgca
    16801 gcgcgaatgg gttcgcctga aaatcgccga aggtcgcacg ctgaaaggcc acaaaatcgg
    16861 tctgacctcg aaagcgatgc aggcaagctc gcagatcagc gaaccggatt acggtgcact
    16921 gctggacgac atgttcttcc acgatggcag cgatatcccg accgatcgct ttatcgtgcc
    16981 gcgcattgaa gtggagctgg cttttgtgct ggcaaaaccg ctgcgtgggc caaactgcac
    17041 gctgttcgac gtttacaacg ccacggacta tgtgatcccg gcgctggagc tgatcgacgc
    17101 tcgctgccac aacatcgatc cggaaaccca gcgcccgcgc aaagtattcg acaccatttc
    17161 cgataacgcc gccaacgccg gagtgatcct cggaggtcat ccgatcaaac cagacgaact
    17221 ggatctgcgt tggatctccg ccctgatgta tcgcaatggc gtgattgaag aaaccggcgt
    17281 cgccgctggt gtgctgaatc atccggcaaa cggcgtggcc tggctggcga acaaactcgc
    17341 accgtatgac gtacaactgg aagccgggca aatcattctc ggtggttcgt tcacccgccc
    17401 ggttccggcg cgtaagggcg acaccttcca cgtcgattac ggcaacatgg gctccatt
//
LOCUS       DADMGC010000080        17395 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.080, whole genome shotgun sequence.
ACCESSION   DADMGC010000080 DADMGC010000000
VERSION     DADMGC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.080"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(768..1964)
                     /gene="hemY"
                     /locus_tag="JNZ73_002639"
     CDS             complement(768..1964)
                     /gene="hemY"
                     /locus_tag="JNZ73_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HAY8732872.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1967..3172)
                     /gene="hemX"
                     /locus_tag="JNZ73_002640"
     CDS             complement(1967..3172)
                     /gene="hemX"
                     /locus_tag="JNZ73_002640"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY8732873.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(3194..3934)
                     /gene="hemD"
                     /locus_tag="JNZ73_002641"
     CDS             complement(3194..3934)
                     /gene="hemD"
                     /locus_tag="JNZ73_002641"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HAY8732874.1"
                     /translation="MSILVTRPSPAGEELVRRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFTIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3931..4872)
                     /gene="hemC"
                     /locus_tag="JNZ73_002642"
     CDS             complement(3931..4872)
                     /gene="hemC"
                     /locus_tag="JNZ73_002642"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAY8732875.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5259..7805
                     /gene="cyaA"
                     /locus_tag="JNZ73_002643"
     CDS             5259..7805
                     /gene="cyaA"
                     /locus_tag="JNZ73_002643"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HAY8732876.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7845..8165)
                     /gene="cyaY"
                     /locus_tag="JNZ73_002644"
     CDS             complement(7845..8165)
                     /gene="cyaY"
                     /locus_tag="JNZ73_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HAY8732877.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8213..9526)
                     /locus_tag="JNZ73_002645"
     CDS             complement(8213..9526)
                     /locus_tag="JNZ73_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HAY8732878.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9523..9828)
                     /locus_tag="JNZ73_002646"
     CDS             complement(9523..9828)
                     /locus_tag="JNZ73_002646"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732879.1"
                     /translation="MFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFD
                     GAQLWRYRTADPKQVRLIWKEQHSGYVYGVNYKTGYLDGKITDDGEFIPAPRNEVTK"
     gene            complement(9850..11001)
                     /locus_tag="JNZ73_002647"
                     /pseudo
     CDS             complement(9850..11001)
                     /locus_tag="JNZ73_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11190..11393
                     /locus_tag="JNZ73_002648"
     CDS             11190..11393
                     /locus_tag="JNZ73_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8732880.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11430..12254
                     /gene="dapF"
                     /locus_tag="JNZ73_002649"
     CDS             11430..12254
                     /gene="dapF"
                     /locus_tag="JNZ73_002649"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAY8732881.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12251..12958
                     /locus_tag="JNZ73_002650"
     CDS             12251..12958
                     /locus_tag="JNZ73_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HAY8732882.1"
                     /translation="MKQLGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12955..13851
                     /gene="xerC"
                     /locus_tag="JNZ73_002651"
     CDS             12955..13851
                     /gene="xerC"
                     /locus_tag="JNZ73_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAY8732883.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13851..14567
                     /gene="yigB"
                     /locus_tag="JNZ73_002652"
     CDS             13851..14567
                     /gene="yigB"
                     /locus_tag="JNZ73_002652"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HAY8732884.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14651..16813
                     /gene="uvrD"
                     /locus_tag="JNZ73_002653"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14651..16813
                     /gene="uvrD"
                     /locus_tag="JNZ73_002653"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HAY8732885.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACSELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(16960..17352)
                     /locus_tag="JNZ73_002654"
     CDS             complement(16960..17352)
                     /locus_tag="JNZ73_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732886.1"
                     /translation="MRLGGVFYVAGDKVGIVRLDAFKASKDIQFAVQSGAMLMENGVI
                     NPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVEQLLYLGGT
                     ISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         4058 a         4481 c         4603 g         4253 t
ORIGIN      
        1 gttgacgggg tatctgctgc gctggccatg agaggaagag cggcggcgac agcaacaaca
       61 agacgtttgg gtgaaaacga aaattccatg caaaatgctc cggtttcatg tcatcaaaat
      121 gatgacgtaa ttaagcattg ataattgaga tccctctccc tgacaggatg attacataaa
      181 taatagtgac aaaaataaat tatttattta tccagaaaat gaattggaaa atcaggagag
      241 cgttttcaat cctacctctg gcgcagttga tatgtaaggc aggtttatta tatcgcgttg
      301 attattgatg ctgtttttag ttttaacggc aattaatata tgtgttatta attgaatgaa
      361 ttttatcatt cataataagt atgtgtagga tcaagctcag gttaaatatt cactcaggaa
      421 gttattactc aggaagcaaa gaggattaca gaattatctc ataacaagtg ttaagggatg
      481 ttatttcccg attctctgtg gcataataaa cgagtagatg ctcattccat ctcttatgtt
      541 cgccttagtg cctcataaac tccggaatga cgcagagccg tttacggtgc ttatcgtcca
      601 ctgacagatg tcgcttatgc ctcatcagac accatggaca caacgttgag tgaagcaccc
      661 acttgttgtc atacagacct gttttaacgc ctgctccgta ataagagcag gcgttttttt
      721 atgcatcaga aaggtcccgg aggtgctttc ctccgggtga gaaggaacta ctgtggcggg
      781 ttattctgca acgttaacat caaaccgtcg cgacgcatcg ctgcagcttc ttccggcttg
      841 tgcagtctgt ccagcgcgtc ggcaagccat gcgtaatcgt aggcgtccgg acgttgtttc
      901 agcgccgcgc ggaaggcgag cgatgcttcc tgccattcac cgtgcttcat cagtgactga
      961 cccaatgtgc tccacaacag cgggcgatca ccgacgtttt tgatttgctg gcgtagcact
     1021 ttttccagct gttccgggtt attggttttc agacgcggga tcggcagcag taggcgatcg
     1081 tcatactggc gtttcaggcc gtcgatgata atttgctggg cagtatcatg atcgtcacat
     1141 tcaataagat gttccgccat tgccacctgc aacgctacct gatgacgcgt tttccggctt
     1201 tggtttttcc accagttacg caaaccttca ctaccgttat cggcacgcgc ctgatccatc
     1261 aggccaatcc atgcctgttg ttccagcatt gcacgatgtt cttcatcacc aacatgggct
     1321 ttcgccattg atgggatgat atccagcaga gaactccatg cccctgtgcg gatatacgcc
     1381 tgttccgcca gacgtaatac ttcaggatgg cgtggcgtaa cttccagcag cttatctacg
     1441 ccgtggcgtg cagcatggtt ttcattacgg gccagttgca gacgtacacg ggtgatttct
     1501 accggaatgg tgtcattgcc ggccagctcc gctgcgcgtt ccagatgttg gttggcgcgt
     1561 gcttcatcac cacgttgttg cgcggcttcg gcagccagta gatagttcac caccggttgt
     1621 tcagcgtgat cggcattttt cgccatcagc ttttcaactt gctgataatc accttccgcc
     1681 agtttcagca gcgcctgttc ggtctgctta cgcgcacggc gacgcttacg tccgacaaac
     1741 catccacggg tgtgcgcgcc agtgcggaag atccgccgca gtagccactc aatggcaaac
     1801 agcactacca tcgccagaat caatatgatc gccaggcccg tgacgctggt ttcgatattg
     1861 tagttgtcgg tctggatcag cacataaccc tgatgaccgg caatcatcgg gccaaccact
     1921 atccccgcaa tcagcaaaac aaagagtaat aacactttta gcatggttat tctccttgcg
     1981 gcgcggctgc cggagtatca ccttgcggtg caggtgcagg tgcaggtgca ggtgcaggtg
     2041 caggtttagc ttccgttgtc cccgctgccg gttgtgccag caggttacgc acgcgagtct
     2101 gcatcagttt ttccagcatc gcctggcttt gcagggtttc cggaagatcc atcgagatat
     2161 tttgctggct taactggtcc acctcgtcga ggaacgcttt ggtggtggca tcatcagtat
     2221 cgtagtaagc acgtacccag gtggagacgt tctccagcgc ctggcgataa gtctcttcct
     2281 gatgacgcgg tacagcttgt gctgcgacca gcaggcgaga gcgaatattt tcgcgcagat
     2341 agatatcctg atttggcgct aacagcggta cggcggtgtc atcacgacgg cgaatcgtaa
     2401 tgaagttgtc cataaagttc tgccagcttt tttgcagatt gatacgccat tcgctgatgg
     2461 aactggaaag ctcttcaccg tctgaatcca tcggcgaacc atcgctgtca ttatcggcca
     2521 gacgcaggtt atctacctga tttgaaagct gattaagctt aaggatgatg ccgtcataat
     2581 ccacctgcga tactgcagaa aggctggcga tatcatcggt aattgcccga cgaacggtaa
     2641 tcagactcgg gtcattcata tccgccaggc tggcgtctgc acttttcagc aacgctgcag
     2701 aggtcgtgac gtcctgatcg ctccacagct tccgtccggc gagtttcacc agaaaatcgg
     2761 cctgagccag cagccaggtt ttagcatcgc tgccggaaat ggtggcgatc ttttgttgga
     2821 cttcatccaa ctgttttgcc agcgtttctt gctgacgatt cgcctgctta agctgcgcag
     2881 cttgttgctt aataatgcct tccagctcgg ctttttggct ctcctgggct ttttgcaatg
     2941 ccgtcagttg gttagccagg gcatcgctgg tggcggtctg attgacggcc tgttgtttac
     3001 cccagccata caaaccgaca cccgccgcca gagcaatagc gatagccacc gcgctgagaa
     3061 tcaatgcagt attgttctta ctcttttttt ctgttgcgac aggttgtgac gtggtgtcca
     3121 cggcctccct ggtctcttca accacggcgg aggttttttc ttgttccgtc attatggctt
     3181 cctgttatga gagttattgt aatgcccgta aaagcgcatc gttgtcagcg ttatcggcga
     3241 ccttaatgtc ttgccagccc agttcccggg cgagtttcgc caaacgctca ctgacgacca
     3301 atagtcgaca gtgtagtaac cagtgctcac gataccattg tgggatcagc gaccagagtt
     3361 gctgcaacat ttcaccgctg gtaacaacga ccatcgtcac ctcgcgggat tgccagcgca
     3421 tcgcttcttc tgcaccatcg taatggattg cgcatcgttg ataacattca caaaaagtga
     3481 cctcagcacc gcgcaccgtc agggtatccc caattagctc acgaccgcca ttgccacgta
     3541 atatcagcgc acgtttgccc gcaatatttt gtaattcagg taattgtagc aagacttcgc
     3601 tgatttcccg atcctgtggg tagagaatct tctgtccgct tacggtatgt agcgccagtg
     3661 cggtggtgcg tccaatggtg aaataagtag gtagttgggg ccatttacga tcttgctgat
     3721 gcagctgtga ttgggcaaaa gcaaccgcgt gttgcgagag ggcaaacaac agatcgctct
     3781 cccccagcgc tgccagttga tcagcaagtt gcggtaattg tcgacccgga gaaaactcaa
     3841 tcagcggaaa atgccaggcc acctgcccca gtgtgcgcag acgtctcact aactcttctc
     3901 cagcgggaga cgggcgggtg accaggatac tcatgccggg gcctctccgt tatagacttc
     3961 agcgaggatc tcgcgcgcgc cgttattcag tagctcttct gccagcgaaa tccccatttg
     4021 ttcggcatct tgcggcgcac cgcggcgttc accgcgaata atctgcgaac cgtccggcgc
     4081 gccgaccagc gcacatagcc agatttcgcc atcaataagc tcggcgtagc taccaattgg
     4141 cacctgacat ccgccttcga gacgggtatt catggcgcgt tctgcggtaa cgcgcagtgc
     4201 agtttcgtgg tgattcagcg cggcaagcag ctcgcgagtg cgtgtatcat caaggcggca
     4261 ttcaataccg actgcacctt gtcctaccgc cggaagagaa atctcgggtg gtaacgcggc
     4321 gcgaatgcgg gactccagac ctaaacgttt tagtccggct acggcaagaa tgatggcatc
     4381 gtattcgccg ttatctagtt tgctcaggcg agtgccgacg ttgccgcgca gggagcggat
     4441 aatcagatcc gggcggcgtt cagccagttg gcactggcga cgtaaactgg acgtcccgac
     4501 gatactgcct gctggtaacg catccagact gtcatagtta ttggacacaa aggcatcgcg
     4561 aggatcttca cgctcgcaaa tagtgaccag ccccagacct tgcgggaatt caaccggcac
     4621 atctttcatt gaatgtacgg cgatatcagc gcgattctcc aggagcgcga cttccagctc
     4681 ttttacaaat aagccttttc cgcctacttt cgccagcggc gtatcaagaa tcacatcgcc
     4741 gcgcgtcacc atcggtacca gttcaacgac caggcccgga tggctcgcca tcaacttgtc
     4801 tttgacatag tgtgcctgcc agagtgcaag tgggctttgg cgtgtggcaa ttcttaaaac
     4861 attgtctaac atgcttgtta ccgtcattat catccgtggt ccatcctaac atccttgcca
     4921 gagtgatgtc agtgttgtgg tgaaacgtag acgcctgcgc aaaccgtaaa atgaggtctg
     4981 gcagtggatc ctgacaggcg tttcacgccg tcgtaataag gaatttacag agaataaacg
     5041 gtgctacact tgtatgtagc gcatctttct ttacggtcaa tcagcaaggt gttaaattga
     5101 tcacgtttta gaccattttt tcgtcgtgaa actaaaaaaa ccaggcgcga aaagtggtaa
     5161 cggttacctt tgacatacga aatatcccga atgccgcgtg ttaccgttga tgttggcgga
     5221 atcacagtca tgacgggtag caaatcaggc gatacgtctt gtacctctat attgagactc
     5281 tgaaacagag actggatgcc ataaatcaat tgcgtgtgga tcgcgcgctt gctgctatgg
     5341 ggcctgcatt ccaacaggtc tacagtctac tgccgacatt gttgcactat caccatccgc
     5401 taatgccggg ttaccttgat ggtaacgttc ccaaaggcat ttgcctttac acgcctgatg
     5461 aaactcaacg ccactacctg aacgagcttg aactgtatcg tggaatgtca gtacaggatc
     5521 caccgaaagg tgagcttcca attactggtg tatacaccat gggcagcacc tcgtccgtag
     5581 ggcaaagttg ttcctctgac ctggatatct gggtctgtca tcaatcctgg ctcgatagcg
     5641 aagagcgcca gttactacaa cgtaaatgca gcctgctgga aagctgggcc gcctcgctgg
     5701 gtgtggaagt cagcttcttc ctgattgatg aaaaccgctt ccgtcataat gaaagcggca
     5761 gcctgggggg cgaagattgt ggctccaccc agcatatact gctgcttgac gaattttatc
     5821 gtactgccgt gcgtctcgcc ggtaagcgta ttctgtggaa tatggtgccg tgcgacgaag
     5881 aagagcatta cgacgactat gtgatgacgc tttacgcgca gggcgtgctg acgccaaatg
     5941 aatggctgga tctcggtggc ttaagctcgc tttctgctga agagtacttt ggtgccagcc
     6001 tttggcagct ctacaagagt atcgattccc catacaaagc ggtgctgaaa acactgctgc
     6061 tggaagccta ttcctgggaa tacccgaacc cacgtctgct ggcgaaagat atcaaacagc
     6121 gtttgcacga cggcgagatt gtatcgtttg gcctcgatcc atactgcatg atgctggagc
     6181 gtgttactga atacctgacg gcgattgaag atttcacccg tctggattta gtacgtcgct
     6241 gcttctattt aaaagtgtgc gaaaaactca gccgtgaacg cgcctgtgta ggctggcgtc
     6301 gcgcagtgtt gagccagtta gtgagcgagt ggggttggga cgaagctcgt ctggcaatgc
     6361 tcgataaccg tgctaactgg aagattgatc aggtgcgtga ggcgcacaac gagctgctcg
     6421 acgcgatgat gcagagctac cgtaatctga tccgctttgc gcgtcgcaat aaccttagcg
     6481 tctccgccag tccgcaggat atcggcgtac tgacgcgtaa actgtatgcc gcgtttgaag
     6541 cattgccagg taaagtgacg ctggtaaacc cgcagatttc acccgatctc tcggaaccga
     6601 atctgacctt tatttatgtg ccgccgggcc gggctaaccg ttcaggttgg tatctgtata
     6661 accgcgcgcc aaatattgag tcgatcatca gccatcagcc gctggaatat aaccgttacc
     6721 tgaataaact ggtggcgtgg gcatggttta acggcctgct gacctcgcgc acccgtttgt
     6781 atattaaagg taacggcatt gtcgatttgc ctaagttgca ggagatggtc gccgacgtgt
     6841 cgcaccattt cccgctgcgc ttacctgcac cgacaccgaa ggcgctctac agcccgtgtg
     6901 agattcgcca tctggcgatt atcgttaacc tggaatatga cccgacagct gcgttccgca
     6961 atcaggtggt gcattttgat ttccgtaagc tggatgtctt cagctttggc gagaatcaaa
     7021 attgcctggt aggtagcgtt gacctgctgt accgcaactc gtggaacgaa gtgcgtacgc
     7081 tgcacttcaa cggcgagcaa tcgatgatcg aagccctgaa aactattctc ggcaaaatgc
     7141 atcaggacgc cgcaccgcca gatagcgtgg aagtcttctg ttatagccag catctgcgcg
     7201 gcttaatccg tactcgcgtg cagcaactgg tttctgagtg tattgaactg cgcctttcca
     7261 gcacccgcca ggaaaccggg cgtttcaagg cgctgcgcgt ttctggtcaa acctggggct
     7321 tgttcttcga acgcctgaat gtatcggtac agaagctgga gaacgccatc gagttttatg
     7381 gcgctatttc gcataacaaa ctgcacggct tgtcagtgca ggttgaaacc aatcacgtca
     7441 aattaccggc ggtggtggac ggctttgcca gcgaagggat catccagttc tttttcgaag
     7501 aaacgcaaga cgagaatggc tttaatatct acattctcga cgaaagcaac cgggttgagg
     7561 tgtatcacca ctgcgaaggc agcaaagagg agctggtacg tgacgtcagt cgcttctact
     7621 cgtcatcgca cgaccgcttc acctacggct caagcttcat caacttcaac ctgccgcagt
     7681 tctatcagat tgtgaaggtt gatggtcgcg aacaggtgat tccgttccga acaaaatcga
     7741 ttggcaacct gccgcccgcc aatcaggatc acgatacgcc gctattacag cagtattttt
     7801 cgtgatgagc gtgccggaaa gcgaggctta tccggcatgc aatcttagcg gaaactgact
     7861 gtttcacccg cctgttgcgt cgccgcctgt tccagcaaat cccagaaggt ttcgccgctg
     7921 cgatcgcaaa tccattcatc gcctttcagg tcaaaatggt agccgccctg tttggttgcc
     7981 agccacacct ggtgcagcgg ctcctggcgg ttaataatga ttttgctgcc gttttcaaag
     8041 gtaatggtca gtacgccgcc gttgatttcg cagtcgatat cgctgtcgcc atccctgtcg
     8101 tccagacgct cttcaatggt cagccacagt tgatcagcca ggcgatgaaa ttcactgtcg
     8161 ttcattgttg taccctgttt ttaagtgatg gcggcagtat agcggcatgg ggtcagggct
     8221 tcaaagtttg cacctctgct gctgcgttcc ggcatgattc atccgtcacc ggaataatga
     8281 tgtctctgtt ttgaacttca cgtacgttct cctctctgac acgagttttc gccgggcagg
     8341 gggagggcgt aatccttacc tctgtatcgg tccatgtata aacattgcga tcgtcataag
     8401 cgaagcgatc gtttttagtt agcggaccgt taactggaat aatttgcaag ctctttgacc
     8461 gtaattggcg gataaagtaa gcatgctgtt tgccgctatt aaatgagcga tcaatgacca
     8521 agtctggcgt atccagcggg attatctcaa gctggtcttc accaaatgtc gataattttg
     8581 ttacatacag ccgatcttta tattgcgcaa tattgccagt aatcagatgg aattgttcag
     8641 gttcaatacc tggtaggtct atccacttgc tccactgacg gtcgggcgtg agctgtttac
     8701 gccagcgaac gcgactttcc cctacttcaa aagagacgtc atcatctacg ggtatcgctt
     8761 tgcctgataa ttgaccaaag ggataacgat gcagaccctt attgtcgcga aaaaccagca
     8821 gtgaatgagg tatccagcga attatctcaa atgagtctgc attcgccgta aggtgaacgc
     8881 catttaaata aatattctgc ccactgcgga taagcagatc attggaatat gaatttctgt
     8941 ttacactcat aagacgtgga ttaatacccc accatctttt ttctaataca gtaacatcac
     9001 tgctgctccc ctgacgtcga ccggacaagt agagattttt actatccacc agcgtggtag
     9061 agttaccttc gatagctttt agtgtcgcca gatcaacacg tgacgcgccg ctattacttt
     9121 cggtgcgctg tccgtcaaaa tagaggctat atttatcgac cgcgaagcgg cccatagcct
     9181 gaaagctggc aatatcgaca gtgggtgttc ccggtggatt gctaacgatt ttgccacgcc
     9241 agagaataac gcggccgtct gtcacccagt ggtattcctc attttctatg cttatttcat
     9301 cggaaggtat ataacccgtt ttcccagaga tatttttcac aaggcgcaaa ccggccctgc
     9361 tcatctccgg aaactgggca atgattttct tatcccccca ataaccttta ccgtaagaca
     9421 taacaccgtg atcattgtaa tagtcgaacc ctatggcctg tacagcaggc gtcagaaatg
     9481 tgtgtcccaa caggatgagt gcaactgtga ttatttgacc cttcattttg tcacctcatt
     9541 gcgcggggca ggaataaatt cgccgtcatc agtaattttt ccgtccagat atcccgtttt
     9601 gtaatttacg ccatagacat atcccgaatg ttgttccttc cagattaagc gaacttgttt
     9661 cggatcagcg gtgcggtatc gccagagctg cgcgccatca aagctgtaaa tgtagcggtt
     9721 atcgcgggca taccatctgc gcaaaaaggg atggtcatta agatgggctt cgcgttcatc
     9781 cggagaacgg ttatccagta agatcagcgg tccgtcagat tcaaacacgt gtaaaccgga
     9841 atggtatggc tacccttcgg cacgcgtaag taaatagccg tggcgtttcc cggcgttgaa
     9901 acgtttgttg ataataagat tgggatcatc aagtttcacg atctccagct tacggtcacc
     9961 agactctgtt tcgatgatgg tataaagcga gtcctgatac tgggcgacag catcactgat
    10021 gggttgaaac tgctccggat cgagtccggg taattcttcc tgtttacaag caggtccttt
    10081 acgccagaac actttctttt ccagcagaga aaaatccaga cagtgctttg ttaaatcttc
    10141 atcccacgcc agattttcct tgtcgaggac tttacgctgc agcccgtttt tatcacgcca
    10201 ggtcaagagt gagccaggca tccagcgcac gacggaaaag gtgtccgggt cggcatcaag
    10261 ctgttcgccg ttgagcagga ttttggtccg tgtacgagcc agggtatccc aggggccaaa
    10321 tggtacggca atgcaggggt taaacgtggt ggaaaacttt cctcgctgat cccaggactt
    10381 ctgcgccagc acacggaaac tctcggggtc agcgaggcga tgcccattga tatacaagaa
    10441 attagcgtca cgtaatatca gccccagtaa gtcaggatcc cagggaggcc ggaattccac
    10501 ctgatgcagc gttttgatat ccaagctatt tccaccacca ttgtcatcgg tgcgcttgcc
    10561 ttcaaaatag agactgtgtt tatctgcggc aaaatcaccc catgccttga acgaggcgac
    10621 atcgacggga ggtgtgccgg gagggttttg cacaacttca cccgcgtaga ggatattgcg
    10681 accatcgcta tgccatgaat aatgaggata ctaggcctgt gcgccctcat agaggtagtc
    10741 ggttatcaaa tcagtcagag gcggttcttt atcccattcg tctagtgttg tcgggtctat
    10801 taataaattg ggtaaccgac gcaaactctt gtaattcacg tcaggtagag aaagatttgt
    10861 ttctttataa gggcgcaggt acaccacgcc ttttccctgt ttctggtaaa tattatgttc
    10921 aggtgcggaa agccgacatc cccatgccga cgataaaaac aatgaacaag ccaacaaaca
    10981 aaccacattg cgatagtgca taaagccatc ctggcgcgag gtgttgatca cgaaactgcc
    11041 agcagaacat aaatcctcac gagtaagcgt tatactcgca gcatttcctc acttttcaga
    11101 cttcataaag agtcgctaaa cgcttgcttt tacgccttct cctgcgatga tagaaagcag
    11161 aaagcgatga actttacagg caatccataa tgaaaaacgt gtttaaggca ctcactgtat
    11221 tacttactct cttcagcctg acgggctgcg gtctgaaagg tccgctctat ttcccgcctg
    11281 cagataaaaa cgcaccgccg ccgaccaaac cggtagagac gcaaacgcaa tccacggtgc
    11341 cggataaaaa cgatcgcgcc actggcgatg gtccatccca ggtgaattac taaaagtcag
    11401 tttctgtacc cgcgtgattg gagtaaatga tgcagttctc gaaaatgcat ggccttggca
    11461 acgattttat ggtcgtcgac gcggtaacgc agaatgtctt tttttcaccg gagctgattc
    11521 gtcgcctggc ggatcggcac ctgggggtag ggtttgacca gctgctggtg gttgagccgc
    11581 cgtatgatcc tgaactggat tttcactatc gcattttcaa tgctgatggc agtgaagtgg
    11641 cgcagtgcgg taacggtgcg cgctgctttg cccgttttgt gcgtctgaaa ggactgacca
    11701 ataagcgtga tatccgcgtc agcaccgcca acgggcggat ggttctgacc gtcaccgatg
    11761 atgatctggt ccgcgtaaat atgggcgaac ccaacttcga accttccgcc gtgccgtttc
    11821 gcgctaacaa agcggaaaag acctatatta tgcgcgccgc cgagcagaca atcttatgcg
    11881 gcgtggtgtc gatgggaaat ccgcattgcg tgattcaggt cgatgatgtc gataccgcgg
    11941 cggtagaaac gcttggtcct gttctggaaa gtcacgagcg ttttccggag cgcgccaata
    12001 tcggttttat gcaagtggtt aagcgcgagc atattcgttt acgcgtttat gagcgtgggg
    12061 caggagaaac ccaggcctgc ggcagcggcg cgtgtgcggc ggtggcggta gggattcagc
    12121 aaggtttgct ggacgaagaa gtacgcgtgg aactccccgg cggtcgtctt gatatcgcct
    12181 ggaaaggtcc gggtcacccg ttatatatga ctggcccggc ggtacatgtc tacgacggat
    12241 ttattcatct atgaagcaac taggggaaga actgcaggaa acactcacgg agcttgatga
    12301 ccgggcggtt gtcgattatc tgattaaaaa tcctgagttt tttatccgca atgcgcgcgc
    12361 agtagaagcg atacgtgtgc cgcatccggt acgcggcacc gtttcgttgg tcgagtggca
    12421 catggcccgc gcacgtaatc acattcatgt tctggaagag aacatggcgt tgttgatgga
    12481 gcaggctatc gccaacgaag ggctgtttta tcgcctactc tacctgcagc gcagtctcac
    12541 cgccgccagc agtctcgacg atatgctgat gcgctttcac cgctgggcgc gcgatctcgg
    12601 cctggctggt gcgagtctgc gcctgtttcc agatcgctgg cgcttaggtg cgccgtcgaa
    12661 ccacactcat ctggcattaa gccgtcagtc tttcgaaccg ctgcgtattc agcgtttggg
    12721 gcaggaacag cactatcttg ggccgcttaa cggaccggag ctgctggtgg tgctaccgga
    12781 agcgaaagcg gtgggatcgg tggcgatgtc gatgctggga agcgatgctg atttgggtgt
    12841 tgtgctgttt accagtcgcg atgccagtca ctatcaacaa gggcagggaa cgcagttact
    12901 tcatgaaatt gcgctgatgt tgccggagct tctggagcgt tggattgaac gcgtatgacc
    12961 gatttacaca ccgatgtaga acgctaccta cgttatctga gcgtggagcg tcagcttagc
    13021 ccgataaccc tacttaacta ccagcgtcag cttgaggcga tcatcaattt tgccagcgaa
    13081 aacggcctgc aaagctggca acaatgcgat gcagcgatgg tacgcaattt tgctgtacgc
    13141 agtcgccgta aagggctggg agcagcaagt ctggcgttac ggctttctgc gctacgtagc
    13201 ttttttgact ggctggtcag ccagaacgaa ctcaaagcta acccggcgaa aggagtttcg
    13261 gcaccgaaag cgccgcgtca tctgccgaaa aatatcgacg tcgacgatat gaatcggctg
    13321 ctggatattg atatcaacga tcccctcgct gtacgcgacc gtgcaatgct ggaagtgatg
    13381 tacggcgcgg gtctgcgtct ttccgagctg gtggggctgg atatcaaaca cctcgacctg
    13441 gagtccggcg aagtgtgggt gatggggaaa ggcagcaaag agcgccgcct gccgattggt
    13501 cgcaacgctg tggcgtggat tgagcactgg cttgatttgc gcgacctgtt tggtagcgaa
    13561 gacgacgcgc tttttctgtc gaaactgggc aagcgtatct ccgcgcgtaa tgtgcagaaa
    13621 cgctttgccg aatggggcat aaaacaaggg ctgaataatc acgttcaccc acataaatta
    13681 cgtcactcgt tcgctacgca tatgctggag tcgagcggcg atcttcgtgg tgtgcaggag
    13741 ctactgggtc atgccaacct ctccaccacg caaatctata ctcatcttga ttttcaacac
    13801 cttgcctcgg tgtacgatgc ggcgcatcca cgcgccaaac gggggaaata atgcgttttt
    13861 accggccttt ggggcgcatc tcggcgctca cctttgacct ggatgatacc ctttacgata
    13921 accgtccggt gattttgcgc accgagcgag aggcgcttac ctttgtgcaa aattatcatc
    13981 cggcgctgcg cagcttccag aatgaagatc tgcaacgcct gcgccaggcg gtacgggaag
    14041 cggaacccga gatttatcac gacgtgacgc gttggcgttt tcgttcgatt gaacaagcga
    14101 tgctcgacgc cgggctgagt gccgaagaag ccagtgcagg cgcacacgca gcaatgatta
    14161 actttgccaa atggcgcagc cgaatcgacg tcccgcagca aactcacgac accttaaaac
    14221 agctggcgaa gaaatggccg ctggtggcga tcaccaacgg taacgcccag ccggaactgt
    14281 ttggtttggg ggattatttt gagtttgtgc tgcgcgctgg cccgcacggg cgctcaaaac
    14341 cgttcagcga tatgtacttt ttggctgcgg aaaaactcaa cgtgccgatc ggcgagatct
    14401 tacatgttgg ggacgatctc accactgacg tgggtggggc aattcgcagc ggaatgcagg
    14461 cttgttggat cagaccggaa aatggcgatc tgatgcaaac ctgggacagc cgtttactgc
    14521 cacatctgga aatttcccgg ttggcatctc tgacctcgct gatataatca gcaaatctgt
    14581 atatataccc agctttttgg cggagggcgt tgcgcttctc cgcccaacct atttttacgc
    14641 ggcggtgcca atggacgttt cttacctgct cgacagcctt aatgacaaac aacgcgaagc
    14701 ggtggccgcg ccacgcagca accttctggt gctggcgggc gcgggcagtg gtaagacgcg
    14761 cgtactggtg catcgtatcg cctggctgat gagcgtggaa aactgctcgc catactcgat
    14821 tatggctgtg acgtttacca acaaagcggc ggcggagatg cgtcatcgta tcggacaact
    14881 gatgggcacc agccagggcg gcatgtgggt aggcaccttc cacgggctgg cgcaccgcct
    14941 gctgcgtgcg caccatatgg acgccaatct gccgcaggat ttccagatcc tcgatagcga
    15001 agaccagcta cgcctgctta agcgtctgat caaagccatg aacctcgacg agaagcagtg
    15061 gccgccgcgc caggcaatgt ggtacatcaa cagccagaaa gatgaaggcc tgcgtccgca
    15121 tcatattcaa agctacggta atccggtgga gcagacctgg cagaaggtgt atcaggcgta
    15181 tcaggaagcg tgcgatcgcg cgggcctggt ggacttcgcc gagctgctgc tgcgcgctca
    15241 cgaactgtgg cttaacaagc cgcatatcct acaacactac cgcgaacgtt ttaccaatat
    15301 cctggtggac gaattccagg ataccaacaa cattcagtac gcgtggatcc gcctgctggc
    15361 gggcgacacc ggcaaagtga tgatcgtcgg tgatgacgac cagtcaatct acggctggcg
    15421 cggggcgcag gtggagaata ttcagcgttt ccttaatgat ttccccggtg ccgaaattat
    15481 tcgtctggag caaaactacc gctctaccag caatattctg agcgccgcta acgccctgat
    15541 tgaaaacaat aacgggcgtc tgggtaaaaa actgtggacc gatggcgcgg acggtgagcc
    15601 tatttccctc tattgcgctt ttaacgaact cgatgaagcg cgttttgtgg ttaaccgcat
    15661 caaaacctgg caggacaacg gcggggcgct tgccgagtgc gccattctct accgcagtaa
    15721 cgcccagtcg cgtgtgctgg aagaagcact gttgcaggcg agtatgccgt accgtattta
    15781 cggcgggatg cgcttcttcg aacgccagga aatcaaagat gcgctctcgt atctgcgcct
    15841 gattgccaac cgtaacgacg acgcggcctt tgagcgtgtg gtgaatacgc caacgcgggg
    15901 tattggtgac cggacgctgg acgtggtacg tcagacatcg cgcgatcgcc agttaacact
    15961 ctggcaggca tgtagtgaac tgttgcagga aaaagccctc gccggacgtg ctgccagcgc
    16021 cttacagcgg tttatggaac tgatcgacgc cttagcgcag gaaactgccg atatgccgct
    16081 gcatgtacag actgaccggg taattaaaga ctccggcctg cgtaccatgt acgagcagga
    16141 gaagggcgaa aaaggtcaga cgcgtatcga aaacttagag gaactggtga cggcaacgcg
    16201 ccagttcagc tacaacgaag aagacgaaga tttaatgccg ctgcaggcgt tcctatccca
    16261 tgcggcgctg gaagcaggcg aagggcaggc ggatacctgg caggatgcgg tacaactgat
    16321 gaccatgcac tcggcgaaag gtctggagtt cccgcaggtg tttatcgtcg gtatggaaga
    16381 gggcatgttc ccaagccaga tgtcgctgga tgaaggcggg cgtctggaag aagaacgccg
    16441 tctggcctac gttggcgtaa cccgcgcgat gcagaaactg acgctgacct acgcggaaac
    16501 tcgccgtctg tatggcaaag aggtttacca tcgcccgtcg cgctttattg gcgagttgcc
    16561 ggaagagtgt gtggaagagg tgcgcctgcg cgccacggta agccgcccgg tcagccatca
    16621 gcggatgggt acgccgatgg tcgagaacga cagcggctac aagctcggcc agcgtgtacg
    16681 ccacgctaag tttggtgaag gcaccatcgt caatatggaa ggcagcggtg aacatagccg
    16741 tttgcaggtg gcattccagg gccagggaat caaatggctg gtggcagctt acgcccggct
    16801 ggagtcggtg taacgttgcc ggatgcggtg ctgcgcacct tatttggcct aaaaaatcat
    16861 tcagattcaa taaattgcaa cgtcatgtag gcaggataag gcgtttacgc cgcatccggc
    16921 atcagcagca tatttagtat ctgacacaaa gctatcattc taacctttcc gctcgacgga
    16981 aatcatggtg acaaaaggat agcgttgcca cgggattgcc ccgcctttca tatacatatg
    17041 tgaaatcgtg ccaccaagat aaagcagctg ctcaacgttc agtttcgctt tggcataaca
    17101 ggcaaaatca taaaaatttg tcgcctgctg gctcaacaaa aacacggcat tcccatgttt
    17161 attaatccca acaccgttac gaattttacg tgaggcgacg ttgggatgaa tacgcggatt
    17221 aattacaccg ttttccatca acattgctcc tgactgcacc gcaaactgaa tatctttact
    17281 ggctttaaaa gcatccagac gaacgatgcc gactttatct cccgcgacat aaaacacgcc
    17341 gccaagacgg ataaagaaat tcccttcacc tgaagcgaga tttaacgcta ccttc
//
LOCUS       DADMGC010000081        17385 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.081, whole genome shotgun sequence.
ACCESSION   DADMGC010000081 DADMGC010000000
VERSION     DADMGC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.081"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(41..772)
                     /gene="rlmB"
                     /locus_tag="JNZ73_002655"
     CDS             complement(41..772)
                     /gene="rlmB"
                     /locus_tag="JNZ73_002655"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAY8732887.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(952..3393)
                     /gene="rnr"
                     /locus_tag="JNZ73_002656"
     CDS             complement(952..3393)
                     /gene="rnr"
                     /locus_tag="JNZ73_002656"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAY8732888.1"
                     /translation="MSQDPFQGREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="JNZ73_002657"
     CDS             complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="JNZ73_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HAY8732889.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLLE"
     gene            complement(4062..5360)
                     /gene="purA"
                     /locus_tag="JNZ73_002658"
     CDS             complement(4062..5360)
                     /gene="purA"
                     /locus_tag="JNZ73_002658"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAY8732890.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5464..5661)
                     /locus_tag="JNZ73_002659"
     CDS             complement(5464..5661)
                     /locus_tag="JNZ73_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HAY8732891.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="JNZ73_002660"
     CDS             complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="JNZ73_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HAY8732892.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="JNZ73_002661"
     CDS             complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="JNZ73_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HAY8732893.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="JNZ73_002662"
     CDS             complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="JNZ73_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAY8732894.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="JNZ73_002663"
     CDS             complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="JNZ73_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAY8732895.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="JNZ73_002664"
     CDS             complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="JNZ73_002664"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAY8732896.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="JNZ73_002665"
     CDS             complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="JNZ73_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAY8732897.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="JNZ73_002666"
     CDS             complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="JNZ73_002666"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HAY8732898.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVLVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="JNZ73_002667"
     CDS             complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="JNZ73_002667"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAY8732899.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14434..15981)
                     /gene="nnr"
                     /locus_tag="JNZ73_002668"
     CDS             complement(14434..15981)
                     /gene="nnr"
                     /locus_tag="JNZ73_002668"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY8732900.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15980..17119
                     /gene="queG"
                     /locus_tag="JNZ73_002669"
     CDS             15980..17119
                     /gene="queG"
                     /locus_tag="JNZ73_002669"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAY8732901.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17102..17155)
                     /locus_tag="JNZ73_002670"
     CDS             complement(17102..17155)
                     /locus_tag="JNZ73_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732902.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3940 a         4956 c         4370 g         4119 t
ORIGIN      
        1 aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc tgacgaaccg
       61 cttcaaataa gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca
      121 ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt
      181 ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg
      241 cctcgcctgc cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg
      301 caaggttagt cacccgaatc agcggaacgc tttctgccgc gccgcaggct acttttttcg
      361 ccgtagcgtt gagctgtgcg gagcgatctt tcggcacaat caccgcatga acgcctgcgg
      421 cgtccgcact acgcaggcac gcgccgaggt tgtgcggatc ggttacgccg tcgaggatca
      481 gcaggaacgg ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgac
      541 gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt ttctcgtcga
      601 gatattggcg gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg
      661 gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg
      721 cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc attaatgtac
      781 tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat ctctgcaata
      841 tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc cggcatgaac
      901 aaagcgcatt ttgtcagcaa tctaaccctc ttcttttaaa gagggtattg atcactctgc
      961 cacttttttc ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc
     1021 cgatggcttt ttcgcttttc tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc
     1081 accgcggaag gcgctgtctg gctcaaagtt taccttttta ccgacctgac gacgcttgcc
     1141 gccttttttg cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg
     1201 cggtgcgcgt tcactggaga tcagactaaa gtcgattttg cgctcgtcca tattaaccgc
     1261 ttcgacgcga acttccacgc gatcgcccag gcgataagtc tggccgctgg attcccccat
     1321 caggcgttgc cctacctggt caaagcgata gtagtcattg tccagcgaag agacatggac
     1381 cagaccatca atgaacaagt cgtccagacg gacgaagaag ccaaagccag tgacgctgga
     1441 aattacgcct ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc
     1501 agcgacatcg cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccag
     1561 ttgcaacatc tcttccatcg aataatggta gccgccggtt tcagtggtgt tgccctgatg
     1621 cccctgctct ttcgccagca gatatttaat ggcgcggtgc agcgtcaggt caggataacg
     1681 acgaatcggc gaagtaaagt gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt
     1741 ttctggatcg taaatcgcct gtttcatcga gcgtagcagc atggtttgca gcatttctgc
     1801 atcaggacgg tcggcaaccg actccagcag ttccgcgtag tcacgcggtt ccggcttgtt
     1861 accacccggc agctccagcc ccagctccgc cagcactgaa cggaaagagg taatcgcttc
     1921 ggtgctcggc ttgtcgtgaa tacggaacag tgccggttct ttcgctttct caacgaaacg
     1981 cgccgccgag atattcgcca gaatcatgca ctcttcaatt aatttgtgcg cgtcgttacg
     2041 ctgggtctgt tcgatacgtt caatacggcg ttcagcgttg aaaatgaact tcgcttcttc
     2101 gctctcaaat gagatcccac cgcgttcttc acgggcttta tccagcactt tatagaggtt
     2161 atgcaactct tcgagatgct taaccagcgg ggcgtactgc tcgcgcagat cctgatcgcc
     2221 ctgcagaata tgccagactt tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc
     2281 gtagaatttg tagcccgtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca
     2341 caggcggtct acctgcgggt tgagcgaaca caggccgtta gagagcactt ccggcagcat
     2401 cgggataacc tgcgaaggga agtacaccga cgtgccacgg ttacgcgctt ctctgtccag
     2461 cggcgttggc ggacgcacat agtagctgac gtcggcaatc gcgacccata aacgccagcc
     2521 gccgccgcgt tttttctcgc agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc
     2581 aatggtgacc agcggtaaat cgcgcaaatc gacacggccc gcttttgctt cttccggcac
     2641 ttcttctttc agccccgcaa cctgttgctc aacagcctgc ggccagatat acggaatttc
     2701 atgggtacgc agagcgatat caaccgccat gccggtgccc atattgtcgc ccagcacttc
     2761 gacgatttta cccaccgctt tggtgcggcg agtcggacgc tgagtcagtt cgactacgac
     2821 cacaaagccc atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagctcag
     2881 acggctatcg tcaggaacca caaagccgac gcccgcttcg gtaaagtagc gaccaacaat
     2941 ctggctggtt tttggcacca gtacgcggac aatacgcgct tcacgacgac ctttacggtc
     3001 agcacccagc ggctgcgcca gcacctgatc gccatgaatg caggttttca tctgctcgct
     3061 ggagagatac aaatcatctt tacgcccttc aacccgcaga aagccgtagc catcacggtg
     3121 gccaataacg gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacgacg
     3181 agtgaagacc agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag
     3241 ctgctcttcg ccttcaatgt gcagttctac cgccagctca tcacggctgg ccggtttttc
     3301 acgtttggtt aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc
     3361 agcttcgcgt ccctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat
     3421 gaagattttc gtcactccaa cagcaataat ttataaagcg gttgattctc ttcaaccaaa
     3481 tcggcaagcg tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc
     3541 tgtttcaacc gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat
     3601 aagggctcca gctcgcgcac cacatcacca atacgtatcg cactcgccgg tttacccagg
     3661 cgaatgccgc cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg
     3721 attattttga ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg
     3781 gtcatccgcc cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg
     3841 aaactcgtta actgcacatc aacctcaaaa gggaaatcgg gaaaaaatac atttatattg
     3901 atgataaacc agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc
     3961 gaggatttaa cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac
     4021 tgttagcccg gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacgggtcg
     4081 cgcagaatca tggtttcagt acgatccgga ccggtagaga tgatatcgat cggcacaccg
     4141 gtcagctctt caatacgctt gatgtagttc agcgccgcct gcggcaggcc gctacgatct
     4201 ttcacgccga aagtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct
     4261 ttccagtcgt cagctgccag cggagtggta gtcacttcgc gaccatccgg catacggtaa
     4321 gccacgcaga gtttaacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag
     4381 ccagacaggg agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta
     4441 cgacgacgac gacccgtagt tgcaccgaat tcgttaccct gcttgcagag gaactcgcca
     4501 gtttcatcaa acagttcggc cgggaaagga cctgcaccta cacgagttga gtaagctttg
     4561 aggataccca gaacgtaatc aacataacgc gggcccaggc cggaaccggt cgccacgcca
     4621 ccagcagtgg tgttggaaga agttacgtac ggataagtac cgtggtcgat atccagcagc
     4681 gtaccctgcg caccttcgaa catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg
     4741 tcagaaacgt caaccaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga
     4801 actttctggt aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat
     4861 tccatcactt ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc
     4921 agaccgcgac gtgccacttt atcttcataa gcaggcccga taccacgacc ggtggtgccg
     4981 atcgctttcg cgccacgcgc tttctcacgc gcgttatcca gcgcaacgtg ataatcaagg
     5041 atcagcggac atgcttcaga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc
     5101 agttctttca tctctttcat cagcgcggcc ggagacagca caacaccgtt accgatgatg
     5161 ctggttacat tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg
     5221 ttgattacga gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc
     5281 cgttcagtca gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt
     5341 acgacgacgt tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat
     5401 cgctttttca gagttacagc actttttgca ctcaaaaatg acctcgaaca gtctgttttt
     5461 tgttcagcca atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc
     5521 accgccaaaa cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt
     5581 ccatgccttc gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc
     5641 cagccagatt gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct
     5701 gaggatgcgg tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt
     5761 cggcgtcttc atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt
     5821 gccagagaag ctgttctcat aagcacgcag gctacggatg aatgcgtaga agtccggatc
     5881 tttactgaat gcatcagcaa acagtttggc tgcttcggca tcgccttcac cacgcataat
     5941 gcggccctga cgctcagctt ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg
     6001 cagtttttcc gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc
     6061 ggcgcgcata cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttgat
     6121 acgcacatcg acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac
     6181 tttgcccttc gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatctgctgc
     6241 cggggtagta acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc
     6301 gagggtcaga cgaccacggg aatcggtgac gatatctttc acgtccaggc gaccaatttc
     6361 agaacgcaga cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc
     6421 accacccgtt gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagagtc
     6481 gacgatcagg tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg
     6541 aatacgtgcg tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagaccagg
     6601 ctcataaacc aaaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat
     6661 accacgctca ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat
     6721 gataatcgcg ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc
     6781 tgcgcgttgg cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt
     6841 gctccactgg ttgtggagga agaggctggc ggcagacgca gcagattgct ggcaccgtta
     6901 tcgctcttcg ccgcaggggc gttaccacct ttcagcatct ggtctaacgg cagaaccatc
     6961 aggttgccac ctttatcgtt aaccagcact ttgcgggtgt tacccaacac tttttccatc
     7021 gtctcgatat acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt
     7081 ttagcaaagc gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc
     7141 gcctcttcga ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggtatac
     7201 gcttctgctt cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac
     7261 gccgctttta cttcttccgg cggacgagca gcctggaagt tgacgtccag cagcgtgata
     7321 cccatgtcat acggacgaat cgtctcttcc agttcgcgct gagtatcgct acgaatcacg
     7381 gtacgacctt ccgtcagaat gcggtccatg gtgtatttac cgataactcc acgcagggcg
     7441 ctgtcggtag cctgacgcag gctgtcatcc gggctggtca cgctatacag atatttttcc
     7501 ggattggtga cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc
     7561 agcatcacac cagaagcggc cagttcacgc acggcttcca cgttcaccgg tttgacttcg
     7621 tcgataaacg tcggtttcca gttcagaccc ggctcaacca gatggctgaa tttaccaaag
     7681 cgtgttacca cgccgcgttc ggcttcttta atggtataga aaccactggc cgcccagata
     7741 atgacaatcg ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct
     7801 tgcgatgaac tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt
     7861 ttgctcagtt tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg
     7921 tttccatttc cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt
     7981 ccgttattac cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc
     8041 cctgttgtct ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagat
     8101 caggtaatcg atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat
     8161 acgaacttgc agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag
     8221 ctgataaaaa cgactcctca gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc
     8281 cacctcgccg gaaagccgct ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt
     8341 ctgtgcggaa agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc
     8401 gaaatcttcc agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc
     8461 gatctcttca agaaccgtat tcaccgcttc gatgttttct tgtacacgca catccgccgc
     8521 atcaatgacg tgcagcagta atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc
     8581 tgccaccaga tcgtgcggca ggtggcgaat aaaccctacg gtatctgcaa gtacggtttc
     8641 accgacatct gcaacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc
     8701 tgccgcgtag acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt
     8761 atatcccacc agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc
     8821 acgctgcttt tcaactcttt ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa
     8881 acgacggtcg gtttcgagct gggtttcacc cggaccacgc aaacctatcc cgcctttctg
     8941 tctttcaagg tgggtccagc cacgcaccag gcgcgtagcc agatggcgca gctgcgccag
     9001 ctcaacctgc aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag
     9061 gccggtgcgg tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg
     9121 gctcagggca tggtcaaaaa gaacgaccga agcacccgtc gctttgacag cttccgcaat
     9181 ttcaactgct ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt
     9241 aatcacctgc aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc
     9301 ttccatatct ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc
     9361 ataacggtca aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg
     9421 gctccccgtg taaaaaaaca gcccgaaacc ttattcggtt tcttcgctgt cctgttgcgc
     9481 ggaagtattc tgtgcgctgc taccatgatg gtagttactg ctggtaccgc caccggcgtt
     9541 gttactgtga tgagaaaccg ggcgagatgg gacaacagta gaaatcgcgt gcttgtaaac
     9601 catctggctg accgtgtttt tcaacaggat cacgaactga tcaaaagact cgatttgccc
     9661 ttgcagctta ataccattca ccaaataaat agaaactgga acacgttccc gacgcagtgc
     9721 gttcaggaac ggatcttgta aagattgccc cttagccatt ctctcttttc cttatatgct
     9781 tatttgtact ttgaaccttt cgattctgaa aaaattgcgc acgatacgtc tcaattgtac
     9841 acattcagcc tgcgatagca ccaacaacct gtaatacttc gtcacgcgcc tgttctggtt
     9901 tttcactgtc aagccagtga accccttccc aaccacgcag ccaggttatc tgccgcttcg
     9961 ccaactgtct cgtggcgcaa acacctcgat aaaccatttc atcgtatgag atttcgcctt
    10021 caaggtaaga ccacatctgg cgataaccca cgcaacgaat ggaaggcaag tccgtatgca
    10081 aatctcctcg ggcaaaaagc gcccggactt ctgcttcaaa acctgaagcc aacatctgat
    10141 gaaaacgctg ctcaatgcgt tgatggagca gttcacggct cgccggggcg atggcgaact
    10201 gatgcacctg atacggtaga gcgtctcctg acgtttgcgt cagttccgtt aaagttttac
    10261 ccgaaataaa aaaaacttcc agtgcccggg aaagcctttg tggatcattt ggatgaatcc
    10321 ttgctgccgc aaccggatct acctcctgaa gttgacgatg caatgactcc caaccttgct
    10381 ctgccgcctg ttgctcaatt ctggcccgca cttccgggtc tgccgacggt agcggcgaca
    10441 acccttccag caatgccttg aaatacaaca tcgtaccgcc cactaacagt gggatccgcc
    10501 ccgccgcggt gatatcggcc atttccgcca gcgcatcgcg gcgaaaatca gcagccgagt
    10561 aagcctgcga cggatcgcga atatccagca atcggtgcgg cgcggcgagt aactcttcag
    10621 cgttcggctt cgccgtcccg atatccatcc ctttgtaaat aagggcagaa tcaacgctta
    10681 tcaactctac tggtaaaatt ttacgcagct caatggctaa cgccgtttta ccggaggccg
    10741 tcggccccat caaaaaaatc gccttaggca ggctcgcctt actgatatca ctcatctttc
    10801 agggctttta tcgccggatg taaatcaaca gattgtaaca gaccacccgg cggcgttttc
    10861 acaagttgcg gacataaccg ttccacgtcc gccagcaggg ttatggcctg tgccattgac
    10921 cactgcgcat gttcgctcat cagatttcgt gcaatccact gcgcaatatt gccaggttcg
    10981 aatacggact gcttcgccag gtagcctatc agttcaggaa tcaagatttg taaattttgt
    11041 tggcgtaagg gtaaaggcac ggccctgatg gtcacatgct gtgcatctga ctggaaatca
    11101 atacccaatt ccgctagggc agactgcgct ttttctaatg ccgatttttc ttcgccagaa
    11161 acttttagcc gcaacggaat cagcagcggc tgggcgcaaa cgggcgcttc acccggcgtc
    11221 aattgtgcct gacgcagcca acgttctgcc actggcaagg ataaaagtga aatgttgcca
    11281 tcgcgctcca gcaacgcaca gtcggaatgg acgatagtca gtacccgacc aaaactctga
    11341 ctgttcgccg caagtgcagg ttcctgcggt tccggcgctt ttggtttttg catcggcgcg
    11401 ggcgtttgca aaagctggcg atacacttca ccttgctgtt tctggtagcc tggctgcgca
    11461 ttcggccagg gggcagccgg acgactgcct gaggcgggcg caggagagta gcgcggagct
    11521 accggctcac gagctgccgg ttctgcaaag tgattgcgcc ccgccgccac gcggttttcc
    11581 gggatggcac gcggtgcggg ttgtggttca tcgtccagcg gtagcggcgt ttccagttgc
    11641 tgttgtagca cgctcagcac gccctgatag ataaaatcat gcaccagacg cgactgatgg
    11701 aaacgcactt cgtgtttggc ggggtgtacg ttgacgtcca cctgatgcgg gtcgatctcc
    11761 agatacaaca caaatgccgg ttgctgatcg gcccccagtt tgtcttcgca ggcctggcgg
    11821 atcgcatgat tgatcaggcg atcgcgcatc atgcgaccat tcacgtagca atactgaatt
    11881 tccgccagtg cgggcgtagt gtgatttgga tcggccaccc agccgcgcag ggtgagatcg
    11941 ccgtgttgcc attcaatcgc cagcgcttgt tcgagaaaag cggtgccgca aatcgcgcct
    12001 aagcgccgtt ctttttgccc gccttccggc actgcgcggt actgacgcac aattttaccg
    12061 ttatgcgaca ggttgatcgt gacgtcgaaa cgcgccagcg caatgcggcg gatgatttca
    12121 tcaatatggc taaattcggt tttctcggtg cgcaggaatt tgcgccgcgc cggggtgttg
    12181 tagaacagat ccagcacctc cagcgtcgtt cccaccggat gcgccgccgg tttaaccgtc
    12241 acgtccatgt cgcgtccttc ggcataggcc tgccaggctt cctgctgttc tgcggtgcgt
    12301 gaagtgagcg tcaggcggga aaccgaactg atactcgcca gcgcctcacc gcgaaagccc
    12361 aggctgataa tggcttcgag atcgtccaga gaggcgattt tactggtggc atgacgagcc
    12421 agcgccagcg ccagctcatc ttttttgata ccgcagccgt tatcacgaat gcggataagt
    12481 ttcgccccac cgcgttcgat atcaatatcg atacgcgtcg cacctgcatc gaggctgttt
    12541 tccactagtt ctttgactac cgacgcaggt cgctcgacca cctcacctgc ggcaatctgg
    12601 ttcgccagtt gtggcggtaa gacctgaatt ggcatcaatc gtccttagtt tggcagcgtg
    12661 cgatctggcg tcgtcaccgt actggcagtt tgtgccgttg caccctgcgg cgcagattgc
    12721 atcggatgcg caaggaagta attgcgcagg cctttgtaaa tggcttctgc cagctgttgt
    12781 tggtaatcgt cgctcgccag caaacgttct tcgctgttgt tgctgataaa accggtttcg
    12841 accagtactg atgggatatc cggcgagcgc agaacgccaa ggctggcgtg ttctggtcga
    12901 cgtttatgta tttcgccaat gcgttgcaac tgactgatca tactggtcgc tacatcatac
    12961 cctacccgct gggaatgacc gaactgtaaa tccagcaccg cctggcttaa ataggggtca
    13021 gactgactgt tcgccagcac atcacccgcc ccacccagca gctccgactg tttctcgtgc
    13081 tgctccagcc agctggccat ttcactgttg gcgcgacggt tagagagcac caatacggaa
    13141 gcgccagtcg cactgcggtt cggtgcggca tcagcgtgaa tcgacacgag gaaattggcg
    13201 ttttgcttat gtgccacatc actgcgcccc atcaccgaga taaagtaatc cccgtcacgg
    13261 gttaaaacgc ctttaaacat cggatcgtca ttgagcaaag tacgcaattt acgcgcgatg
    13321 gcgatggtga catttttctc ccgcgtacca ccggggccga tagcgccagg gtcctgaccg
    13381 ccgtgtccgg catcaatagc gatgataatt ttatcgccag tgttagccgt cgcgcgcgct
    13441 gccggacgcg ttaccgtatt actgctgata acacccgtag tgcggttact ttccgtttta
    13501 aacggattgc gcgccggttc gctgacgcgc ggtgcgacaa ccgcaggcgt ttcaacgcgt
    13561 ttcgcaacca cgggcggcgg aggaggcggt ggcggcacat cggcgttaat cgtaaagacg
    13621 acagtgtaat tgctgccatt ctgccgcttc accgcttcgg ttttaccgtt ttcggtaaga
    13681 tcgaccacca gccgtagcgt ttgtgcatct ttaggcgttc cagagcgaat cgccttcacc
    13741 agattattgc cgctgaacaa caacggcagt ccctgaatca cgcccgtttg tttgatatcg
    13801 agcgccacgg tgcgtttgct ttgatggcta aacgcataat caggatcgcc aataaaactc
    13861 aacgttatcc gtgcctgttg attaccgtta gaaacctgaa tatcagagag cgtcgcggca
    13921 cccaccggcg tgcacagcag cagcagcgtc gctaccaacc aatttctgat gcgatacatc
    13981 atcccgccac ctttcaaagg ttaaccggct aaacgcgcca gcaacaattc acccgcagag
    14041 gaaaccgcac tcacgcgcgc ctcacggcct tgtgcctgat aatcaatgtg tatttcgaca
    14101 tccgggtcag gaagaacacc tgtaccttgt tgtggccact ccacaaggca gatggcatcg
    14161 ttggcaaaat aatcgcggat ccccataaac tccagctcct cgggatcggc aaggcggtac
    14221 aaatcaaagt gatagaccat taagttgtcg agcgtatagg gttcgaccag cgtataagtg
    14281 gggcttttga cattaccctg atgacccaga gcctgtaaaa agccccggct aaaggtggtt
    14341 ttacctgcgc ctaaatcgcc atacagatag attacggttg ctccatcgca ggctttcgct
    14401 acccgctcgc ccaggtctaa tgttgcctgc tcatcaggga gcggaattac tcgattcatc
    14461 atggttttta tcagtcactt ccgggttaac aatacgctgt agcgtggaaa agagatcggt
    14521 tgccagcatc ccgcgcgttc caaaacgcgc cgccagtacg tcagctgccg caccgtgcgc
    14581 gacacagcct gcacaggctg catcatacgg cgacagtttt tgcccaagca atgcgccaat
    14641 aataccagag agcacatcgc ccatgccgcc gctcgccatg cctgcatttc cggcatcaat
    14701 aatgcctaaa gcgtcaggat gggcggcgac cacggttccg gcacctttca gcaccgctac
    14761 gccgccataa cgttgtacca gacgtttggc gcaatgtaag cggtcacttt caatttcagc
    14821 gacggaacag cctaacaacc gtgcggcctc gctaggatgc ggcgtgatca cgcgattgtg
    14881 acgcttatcg ggattgattg ccagcaggtt caatgcatcg gcatcccaca acatcggttt
    14941 gcgaaaattc tcaacttttt gcagggcttt tttcccccac tcttgctggc ccagaccggg
    15001 accaatcacc accacatcgg cccattccag gctttcggta agagagtcca tagtcagttc
    15061 atgcaccatc aattctggtc gtgcagtcag cagcggcgca atgttctcac tgcgggtcag
    15121 tactcggact aaaccagcac cagcacgtag cgccgcttcc cccgtcatac gaatagcccc
    15181 cgccgtgccg tgatcgccac cgataatcac cagccgcccg tgatcgcctt tatgcgaagt
    15241 cgggcgacgc ggtttcagcc agtgagaaag ttgttctgcc gaaaaccgct gaattttcgt
    15301 ctcctgacct gccagccagc tatccagccc cagtgagtca aaatgcagtt gtcctgtaac
    15361 atcccgcgct tttccagtga gcaagcctag tttcagcgca ataaaagtga tggtgtgatc
    15421 ggcgttgatc actgcgcctg gcgtagcgcc ggtttcagcc agcaggccgg aagggatatc
    15481 aaccgccaca atcggcgcag gatgggaatt agcgtggtcg attaactggc taatggattc
    15541 gcggggcgct tgctgcaagc cggtaccgag cagcgcatca acaatcagat ctaccgattc
    15601 gggccagaca atattcgaag catggatctc gccacccgcg tttaaccatg cttcgcgtgc
    15661 cagcgcggcc tcttccggca acggtttgtc gctctcctgg gccaacaacg tgacctcaat
    15721 gccgaccgct ttggccagtc gcgcgaccac gtagccatcg ccgccgttat taccatgacc
    15781 gcacagcacc agccagtggc gggcgtcagg atacgccgaa cgacacacct ggaatgcggc
    15841 ctcgccagcg cgaagcatca gctcatagag tgtgagcccc agcacatctg ccgcctcgcg
    15901 ttctccgcgg cggatatcgt cggcgtacca gacggtgtgt ggtatactta cggggttttt
    15961 cttcattgta tggtccgtca tgtcagagcc cctcgatctc aatcagttag cgcaaaaaat
    16021 taaacagtgg gggctggaac tgggctttca gcaggtaggt attaccgata ccgatctcag
    16081 cgagtccgag cccaaactgc aagcatggct ggacaaacaa taccacggcg aaatggactg
    16141 gatggcacgt cacggtatgc tgcgcgctcg cccccatgag ttattacccg gtacgctgcg
    16201 tgtaatcagc gtgcggatga attaccttcc tgctaacgcc gcatttgcca gcacgctgaa
    16261 aaaccccaaa ctcggctatg ttagccgtta tgcgctgggc cgtgactatc acaaacttct
    16321 gcgcaaccga ctcaaaaagc tgggcgagat gattcagcaa cattgtgttt cgctgaattt
    16381 tagaccgttt gtcgattctg cgcctattct cgagcgcccg ttagctgaaa aagctgggct
    16441 cggctggaca ggtaagcact cacttatcct caatcgcgag gccggttcgt tcttcttttt
    16501 aggcgaattg ctggtcgata ttccgctgcc cgtggatcaa ccagtcgagg aaggatgcgg
    16561 caaatgcgtg gcctgtatga cgatttgccc gaccggtgcc atcgtcgagc catataccgt
    16621 cgatgctcgc cgctgtatct cttatctcac catcgaactt gaaggggcga tcccggaaga
    16681 gttgcgaccg ttaatgggaa accgtattta cggttgcgat gactgccagc ttatctgccc
    16741 gtggaatcgc tattcgcaac tcactacaga agacgatttc agcccgcgta agccgctaca
    16801 cgcaccggaa ctcattgagt tatttgcctg gagcgaagag aagtttttaa aagtcacgga
    16861 aggatcggcg attcgccgta taggtcacct gcgctggctg cgtaatatcg ccgtggcatt
    16921 aggcaatgcc ccttgggatg aaacgatttt gacagcgctg gaaagtcgta aaggtgagca
    16981 cccacttctc gatgagcaca tagcgtgggc gattgcgcag caaatagaga gacgaaatgc
    17041 gtgcatagtc gaagtgcaat tgccgaaaaa acagcgtctg gttcgggtga ttgaaaaagg
    17101 gttaccgcgt gacgcctgat tcatccacag cttgtgaata aaaataaaaa cgcattgcaa
    17161 ttcaagacga ataaaatcgt caagtgatcg actcgacaaa acggaatctt gatttttaac
    17221 ttatttatca ataggtataa aaaataccat tcaccgtgcg aagtttcttt gaaattaaga
    17281 ttacagcctg aggctgtgga taactctgtt tacaagagtt tgtcagagac aacaaaaacc
    17341 agcaataacg catcgcgtcg ctgtggatat tttattgaga gaaga
//
LOCUS       DADMGC010000082        17384 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.082, whole genome shotgun sequence.
ACCESSION   DADMGC010000082 DADMGC010000000
VERSION     DADMGC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.082"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            132..575
                     /locus_tag="JNZ73_002671"
     CDS             132..575
                     /locus_tag="JNZ73_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8732903.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQAHLGHRSSSMTDKYRDGRGREWKDI"
     gene            811..1630
                     /locus_tag="JNZ73_002672"
                     /pseudo
     CDS             811..1630
                     /locus_tag="JNZ73_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="JNZ73_002673"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="JNZ73_002673"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HAY8732904.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTCIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="JNZ73_002674"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="JNZ73_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAY8732905.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="JNZ73_002675"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="JNZ73_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8732906.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /locus_tag="JNZ73_002676"
     CDS             complement(4321..4470)
                     /locus_tag="JNZ73_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HAY8732907.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4599..5387
                     /gene="modE"
                     /locus_tag="JNZ73_002677"
     CDS             4599..5387
                     /gene="modE"
                     /locus_tag="JNZ73_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HAY8732908.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5455..6927
                     /gene="modF"
                     /locus_tag="JNZ73_002678"
     CDS             5455..6927
                     /gene="modF"
                     /locus_tag="JNZ73_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HAY8732909.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7188..8204
                     /gene="galE"
                     /locus_tag="JNZ73_002679"
     CDS             7188..8204
                     /gene="galE"
                     /locus_tag="JNZ73_002679"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAY8732910.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8214..9260
                     /gene="galT"
                     /locus_tag="JNZ73_002680"
     CDS             8214..9260
                     /gene="galT"
                     /locus_tag="JNZ73_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAY8732911.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9264..10412
                     /gene="galK"
                     /locus_tag="JNZ73_002681"
     CDS             9264..10412
                     /gene="galK"
                     /locus_tag="JNZ73_002681"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAY8732912.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10406..11446
                     /gene="galM"
                     /locus_tag="JNZ73_002682"
     CDS             10406..11446
                     /gene="galM"
                     /locus_tag="JNZ73_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAY8732913.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYTNRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11649..12401
                     /gene="gpmA"
                     /locus_tag="JNZ73_002683"
     CDS             11649..12401
                     /gene="gpmA"
                     /locus_tag="JNZ73_002683"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAY8732914.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="JNZ73_002684"
     CDS             complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="JNZ73_002684"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HAY8732915.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13936..14316
                     /locus_tag="JNZ73_002685"
     CDS             13936..14316
                     /locus_tag="JNZ73_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732916.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14430..15371
                     /gene="zitB"
                     /locus_tag="JNZ73_002686"
     CDS             14430..15371
                     /gene="zitB"
                     /locus_tag="JNZ73_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HAY8732917.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(15368..16087)
                     /locus_tag="JNZ73_002687"
     CDS             complement(15368..16087)
                     /locus_tag="JNZ73_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HAY8732918.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="JNZ73_002688"
     CDS             complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="JNZ73_002688"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAY8732919.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKTRADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAVTLRG"
BASE COUNT         4435 a         4539 c         4317 g         4093 t
ORIGIN      
        1 ttgccgaaga agatccagct tctgcaacca ggccattcag aactgaagtg aagcgcagtc
       61 gactgttaat tgacgagtat ctgttaatca ggaaaatagc agacgtccag aacgaatggt
      121 tcggcctttg catggatctt gcactggtaa caggacagcg cgaaggagat ctggcggcta
      181 tgagatggga ggatataaga gatagcaggc tatacgtgga acaacaaaaa acaggggcaa
      241 aaattcgcat ttctctcccc accacgattt ccagattgaa tttaacactt gccgatgtac
      301 tggataactt gaaaaaaata aacggtaaaa acgaaaagtt actcggaggg aagacagcca
      361 ggacaatcgc agcacagttc aggattgccc gagacacatc cggattgaaa tgggaaggtg
      421 acccaccgcc atttcatgaa atccgaagtt tatccggtcg cctgcactca gcagaaaaag
      481 gcagtgattt cactcaggcg caccttggac atcgttcatc cagcatgacg gataaatacc
      541 gtgacggacg agggagggaa tggaaagaca tttagaatta atttcgaatg atttcgaaat
      601 accacttgta atcaattgat tataaaggat taattcataa agagcattta cttcatgaat
      661 gctcctttgc atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat
      721 gctgaattaa aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg
      781 aaaattctta accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta
      841 gacggcacct tattgacccc gaaaaagacc ttgcttccat catcgataga agccctggcc
      901 cgcgctcgcg aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt
      961 catccttttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcaccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgcag gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagtaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattaccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagct gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc attcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     4561 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     4621 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca
     4681 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     4741 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
     4801 caggcggtaa aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     4861 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gatgatgacg
     4921 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
     4981 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
     5041 atgtcttact ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     5101 cacgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     5161 gcattactca ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta
     5221 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa
     5281 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca
     5341 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
     5401 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     5461 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     5521 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag
     5581 tcggcgctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     5641 cagttttccc acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa
     5701 tggcaacgga ataacaccga tatgctcggc cctagcgaag atgacaccgg acgcactacg
     5761 gctgagatta ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag
     5821 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     5881 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
     5941 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     6001 cagtccggta ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc
     6061 cagtttgctg gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg
     6121 ctccagcaag cactcgtcgc gcaacttgcg catagtgaac agcttgaagg tgtgcaactg
     6181 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg
     6241 ctgaacaatg gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag
     6301 gtgaatccag gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta
     6361 ttaagcctga ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc
     6421 cgacgtcgcg gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc
     6481 agtagtttgc atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc
     6541 tattttgatt cgattggcat ttatcaggcc gtttcggatc gccagcaaaa actggtgcag
     6601 cagtggctgg atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt
     6661 tcctggggac agcagcgtct ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     6721 cttattctcg atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt
     6781 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct
     6841 gaagatgcgc ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat
     6901 cgctatgtgc tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta
     6961 agcgccctct ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga
     7021 taaaaagcgc aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa
     7081 cggctaaatt cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct
     7141 ttgttatgct atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg
     7201 ttaccggtgg tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc
     7261 atgatgtcat cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg
     7321 agcgtttagg cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga
     7381 tgaccgagat cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag
     7441 ccgtcgccga atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc
     7501 tgcgcctgat tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg
     7561 ccaccgttta tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac
     7621 cgcaaagccc ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa
     7681 aagcccagcc ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc
     7741 cgtcgggcga tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg
     7801 cccaagttgc tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg
     7861 aagatggtac tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg
     7921 tggcgatgga aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg
     7981 taggcagcag cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta
     8041 actatcattt tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca
     8101 aagccgaccg tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca
     8161 cctggcactg gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc
     8221 aatttaatcc cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc
     8281 tggtttcacc gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac
     8341 aggtgttacc tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag
     8401 gcgataaaaa ccccgattac accgggactt acgttttcac taatgacttt gcggccttga
     8461 tgtctgacac gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc
     8521 gcggtaccag ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga
     8581 gcgttgcggc attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa
     8641 aaacgtaccc gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc
     8701 cgcatccgca cggacagatt tgggcaaata gcttcctgcc taacgaagct gagcgcgaag
     8761 accgcctgca aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc
     8821 agcgcgagct ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg
     8881 tgccttactg ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc
     8941 ggatcaccga tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga
     9001 ccagtcgtta tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg
     9061 caccatttaa tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc
     9121 tgctgcgctc cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc
     9181 agcgagacct gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt
     9241 ttcgcgaatc cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca
     9301 acgcatttgg ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg
     9361 gtgaacacac cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg
     9421 tgatcagctg tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa
     9481 atcagctcga cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg
     9541 cgaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg
     9601 tggacatggt gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac
     9661 tggaagtcgc ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac
     9721 aaatcgcgct taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca
     9781 tggatcagct aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct
     9841 cactggggac taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta
     9901 acttcaaacg taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg
     9961 gtgcgcgttt cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaacgctg
    10021 ttgcacatga gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg
    10081 cccgcaccgt tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt
    10141 tgatggcgga gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg
    10201 acactctggt agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg
    10261 gcggcggatt tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac
    10321 agcaagctgt cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt
    10381 gtaaaccatc acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg
    10441 tcagccgtac cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga
    10501 ctggggtgcg actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc
    10561 gctgctcggc tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc
    10621 tattggtcgt tataccaacc gcatcgccaa tagtcgttat acctttgacg gtgaaaccgt
    10681 gacgctttcg ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa
    10741 acgtcgctgg cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga
    10801 tgatggcgat cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga
    10861 cgataaccgt atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat
    10921 gactaatcac gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt
    10981 gcagattctg gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt
    11041 gaaatctgtc gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt
    11101 tcttgccgac gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc
    11161 caaaggcgat ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattacagct
    11221 gaaggtctac accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac
    11281 accgtcgcgg ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt
    11341 tctgccggac agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg
    11401 cgaagagtat tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt
    11461 catgagggct ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    11521 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    11581 gctgttgctt cgtcacggca atataatgag aattattatc attaaaagat gatttgagga
    11641 gtaagtatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    11701 aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag
    11761 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt
    11821 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    11881 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    11941 tgaacaaagc ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg
    12001 gttttgcagt gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc
    12061 cgcgttacgc gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca
    12121 ttgaccgcgt gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc
    12181 gcgtaatcat cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca
    12241 tgagcgaaga agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt
    12301 tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg
    12361 cgaaagcagc agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa
    12421 tgagaaagcc gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac
    12481 attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac
    12541 tctgacggcg catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcgccag
    12601 ttgacgtaac agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc
    12661 gtaggccagc ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac
    12721 catcacgcca ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac
    12781 atccatctgc tttttgaatt gtttggacga attagcatgg ctgaaatcga tcatcacctg
    12841 agctggcaga cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta
    12901 gttaggctct ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac
    12961 aatcgccgaa tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt
    13021 aatggcatcg atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca
    13081 agaaagacca gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc
    13141 gccccagctc atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc
    13201 cgctggcaga ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc
    13261 gttgatctgg aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt
    13321 ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc
    13381 acgcagcgcc agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca
    13441 cgggccaatc accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt
    13501 tcgggcatga gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc
    13561 gacaggagga agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat
    13621 gtctgttcca gtgtcgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc
    13681 cgtcagaatg tgtaaacggg gttttacact atgaacggat taatcctaga attagcaaga
    13741 aaaacgccag attgtcgcga aaaacgagat ctttcctaca atttctaact gtaattcctt
    13801 tgtttgttaa ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac
    13861 caatgcctta tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca
    13921 taacaggagc atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta
    13981 agccttgcca gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag
    14041 gcaaacgccg cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca
    14101 aataacgtcg acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat
    14161 ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc
    14221 aaagatggtc gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat
    14281 gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg
    14341 agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt
    14401 aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac
    14461 acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt
    14521 ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg
    14581 cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt
    14641 cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg
    14701 cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag
    14761 aacgcttccg cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg
    14821 ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc
    14881 tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta
    14941 ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc attctctcga
    15001 tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg
    15061 aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc
    15121 gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc
    15181 cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc
    15241 agatccaaca ctacctgatg gatcactatc agattgagca cgccaccatt cagatggaat
    15301 atcaaccttg tcatgggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc
    15361 atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc
    15421 ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc
    15481 cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt
    15541 ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat
    15601 cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt
    15661 gcatgatcat gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca
    15721 tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc
    15781 agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt
    15841 aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa
    15901 agaaaataat gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct
    15961 ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg
    16021 agagatcata accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa
    16081 aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta
    16141 accgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg
    16201 acatgaacct cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggcca
    16261 ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt
    16321 ggtgcttcca gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga
    16381 tcggttgcca caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg
    16441 gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt
    16501 atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaattcatca
    16561 tgcacaatgc aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa
    16621 cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca
    16681 ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgtttt taccgcagca
    16741 gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgcaaaa tgcgttaaat
    16801 tcttcaacag ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt
    16861 gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac
    16921 aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag
    16981 ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc
    17041 accgcattac gttcttttag cagacgtttt atcttctcgc ggtaatacgc tttttcatca
    17101 atgcttaacg gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt
    17161 acgctcatct taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat
    17221 tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa
    17281 agtcactcgc attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac
    17341 gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atag
//
LOCUS       DADMGC010000083        17103 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.083, whole genome shotgun sequence.
ACCESSION   DADMGC010000083 DADMGC010000000
VERSION     DADMGC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.083"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..170
                     /locus_tag="JNZ73_002689"
     CDS             <1..170
                     /locus_tag="JNZ73_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HAY8732920.1"
                     /translation="SRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAV
                     FGGELTLVQLQ"
     gene            complement(171..446)
                     /locus_tag="JNZ73_002690"
     CDS             complement(171..446)
                     /locus_tag="JNZ73_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HAY8732921.1"
                     /translation="MAGYLSWLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDVYNTEQQCLYSMSDQRIRHGGCFPIEDFIDGFWRPAQEYGDF"
     gene            complement(467..703)
                     /locus_tag="JNZ73_002691"
     CDS             complement(467..703)
                     /locus_tag="JNZ73_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HAY8732922.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDARIMRLA"
     gene            complement(821..2260)
                     /gene="rsmF"
                     /locus_tag="JNZ73_002692"
     CDS             complement(821..2260)
                     /gene="rsmF"
                     /locus_tag="JNZ73_002692"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HAY8732923.1"
                     /translation="MAQHAVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFGGRVFGASVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2340..4838)
                     /gene="yebT"
                     /locus_tag="JNZ73_002693"
     CDS             complement(2340..4838)
                     /gene="yebT"
                     /locus_tag="JNZ73_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HAY8732924.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTITIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLMTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKV
                     TGEMTVDPSVVTLLRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVV
                     VPGEKALLQEPDVLTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAI
                     EPQHRELVKGDSKFVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASY
                     PLYANLEKALENSLSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAF
                     DIDLHIKPEYRNLLTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGA
                     SASQRKGDKRILYASETAARAVGRQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLI
                     TARNEVQAKAVLYPEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGN
                     PRRDFELEEATITDSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLS
                     DRVMIAMRISKRYQHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATP
                     PGTPLAPKAQEGKHFLLQESEPKEWREWGTALPK"
     gene            complement(4807..6091)
                     /gene="yebS"
                     /locus_tag="JNZ73_002694"
                     /pseudo
     CDS             complement(4807..6091)
                     /gene="yebS"
                     /locus_tag="JNZ73_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
     gene            6221..6718
                     /locus_tag="JNZ73_002695"
     CDS             6221..6718
                     /locus_tag="JNZ73_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAY8732925.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6815..7513
                     /gene="proQ"
                     /locus_tag="JNZ73_002696"
     CDS             6815..7513
                     /gene="proQ"
                     /locus_tag="JNZ73_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HAY8732926.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7533..9581
                     /gene="prc"
                     /locus_tag="JNZ73_002697"
     CDS             7533..9581
                     /gene="prc"
                     /locus_tag="JNZ73_002697"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HAY8732927.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMMAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9773..10654
                     /gene="htpX"
                     /locus_tag="JNZ73_002698"
     CDS             9773..10654
                     /gene="htpX"
                     /locus_tag="JNZ73_002698"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HAY8732928.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10700..12073)
                     /locus_tag="JNZ73_002699"
     CDS             complement(10700..12073)
                     /locus_tag="JNZ73_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8732929.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12250..13041
                     /gene="kdgR"
                     /locus_tag="JNZ73_002700"
     CDS             12250..13041
                     /gene="kdgR"
                     /locus_tag="JNZ73_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HAY8732930.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13184..13423)
                     /locus_tag="JNZ73_002701"
     CDS             complement(13184..13423)
                     /locus_tag="JNZ73_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732931.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            13582..13725
                     /gene="mgrB"
                     /locus_tag="JNZ73_002702"
     CDS             13582..13725
                     /gene="mgrB"
                     /locus_tag="JNZ73_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HAY8732932.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13800..14087
                     /locus_tag="JNZ73_002703"
     CDS             13800..14087
                     /locus_tag="JNZ73_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732933.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14757..14900
                     /locus_tag="JNZ73_002704"
     CDS             14757..14900
                     /locus_tag="JNZ73_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HAY8732934.1"
                     /translation="MCGIFSKEVLGKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14913..15122
                     /gene="cspE"
                     /locus_tag="JNZ73_002705"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14913..15122
                     /gene="cspE"
                     /locus_tag="JNZ73_002705"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY8732935.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15288..16097
                     /gene="rlmA"
                     /locus_tag="JNZ73_002706"
     CDS             15288..16097
                     /gene="rlmA"
                     /locus_tag="JNZ73_002706"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HAY8732936.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16094..16660)
                     /gene="mntP"
                     /locus_tag="JNZ73_002707"
     CDS             complement(16094..16660)
                     /gene="mntP"
                     /locus_tag="JNZ73_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HAY8732937.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGSDDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4522 a         4134 c         4314 g         4133 t
ORIGIN      
        1 gtagccgctc gcgattaggt gaacgcccaa aagggcaggg aattacaggt gctgatcatt
       61 tctggtttgg tcatacaccg ttgcgacatc gcgtggatat tggcaacctg cattatattg
      121 ataccggtgc tgtctttggg ggcgaactga ctcttgtgca actgcaataa ttaaaaatca
      181 ccatactcct gtgctggtcg ccagaaacca tctataaaat cctcaatcgg aaaacaaccg
      241 ccatggcgga tccgttgatc gctcatagaa taaagacact gctgttccgt gttgtagaca
      301 tccacaacaa tatcttcaca accgccatcc aggtagcaaa caaaaagtac cagcgcgaac
      361 atttcatccc cgaagtgtgg tgccgtaccg ttaagtttag gagagatttt acaacggggg
      421 aataaccagg acaaataacc cgccatgccg acgggttctt tttggatcag gcaagacgca
      481 taatcctggc atcagattgt tgatcgtaac gggtacggta aaggacagag tgttcgccat
      541 ttagcagggc agggatactg gtttcagcat cccaggcatc cagttgcata cataaatcgg
      601 ggtcagattt acaaggaatg gttaacgttc gatcacccgg cgattcaccg tgtaattcgc
      661 cgtcgtcgat ttcaaaagcg cctatgcgca cactggtttt catcttcgtg ctctccattt
      721 agctgctgat tgtggtacga ccagtccgat cgcacaattt ttcagcctag ttgaggccgc
      781 cgaatgtgcc agtcaaaaag tgcgcttttt ttgtgcgctg tcatgcgtta ctggtaaaaa
      841 gtttcccgtc ccgcaccagt tcacgcgggt agctgttttt cagtcgcgaa ccaatccgtt
      901 tggctaaacc aatcggctga tgctggaaag taaccaacac gtcatccgcc actggcgcgg
      961 cttgcgggta aacatcgcgc ccgcgatacc actcctccgc ttcctgcggt gtcagttcaa
     1021 aagcgtttac attgtcgggg gaggcatggg caataaccgc ttcatgctgc cagcgataac
     1081 ctttgttatg cgtttcggca agtttaatcc ctaagcgaga aaatcggact ttaccgatca
     1141 gggcttcaat gcctaccggg aacaaccaca gttctttgtc gcgttgccag agtcgcaggt
     1201 ttccatccca gtttaagcca acacttgcag ccgcctgacg aatttgtccg gcttcgcgat
     1261 ctttcaccgg gctgaacggg aaattgccca ctttgtattt tggggcgggt aaggcgggga
     1321 tcgcctgagt tttacgcaga cgagcaacga agaagccttc gcagtcgtaa atttgtggga
     1381 aaacatgcaa aaaaccttct tcggtcagcg ccttgtttgc accagggaag agatcgccaa
     1441 gtggtaaaaa ctctacttcg tcggggtaag tctctttcag ccaccggcaa acggcttcgt
     1501 tttcttcccg gtttaaggta caggtcgagt aaaccagcgt accgccagga cgtaatgcat
     1561 gaaaggcgct gtcaatcagt tcccgttggg tagctgcgat ttcctgattg ctttcgggtg
     1621 accagttttt tagcgcatcg ggatctttac gcaccacgcc ttcgccggag cagggagcgt
     1681 ccagcaaaat ggcatcaaac atttctggca ctgacgcacc aaacacgcgg ccaccaaaat
     1741 gtgtgagcgc aacattactg atgccacagc ggctgatatt ggcatgtaac acttttaccc
     1801 gactggcgga aaactcattg gcaaggattg ccccttcgtt attcatccgc gcggcaattt
     1861 gcgtcgtttt tgagcctggc gcagcagcga catccatcac ccgctgtggt gcattaccgt
     1921 cagcaaacaa ggcggcgacg ggcaacattg aactggcttc ctgaatataa aacaggccac
     1981 ttaaatgctc ggtggtacta cccaatggca atgcatcttc attgtcgcgt tcaatccaga
     2041 aaccttcttc acaccacgga attggcgtaa gcgtccagcc ataaggagcg gttaattgca
     2101 ggaaatcagc aacggagatt ttcagcgtat taacgcgaat gctgcggcgc aacgggcgct
     2161 gacaggcggc aagaaaatca tcaaatgaga gcgtcgaagg catagcttcg cgcatttgtg
     2221 tcagaaacgc gtccgggaaa taaacggcgt gttgggccac gagcatgtac cactggcaaa
     2281 aaaacaggcg cgcagtttag cataaacgct ccggcgcagg cacgccggag cagtgggcat
     2341 tatttgggaa gcgcagttcc ccattcacgc cactctttcg gttcactttc ctgcaacagg
     2401 aagtgtttgc cttcctgggc tttcggtgca agtggcgtac ccggaggcgt ggcgaaggcg
     2461 atgccgccac ggataaattg gttaaaggtg ccggttttca ctacgccgcc cgtcagacca
     2521 aagtccagac tgtaacccga tgccaaccag aagacggaat tgttacgcac caggtgttga
     2581 tagcgtttac tgatgcgcat cgcaatcatc acgcgatctg acaatgtccc cagcgtcatt
     2641 ccggtaaccg tacctacttc cagaccacgg aacagcacag gcgtaccgat gcctaacgaa
     2701 ccggcttccg gcgcttcaac aataatgctt aaaccatcta ggtaacgcga atcagtaatg
     2761 gtggcctctt ctaattcaaa gtcgcgacga ggattgccac ggcctggttc gacgttgata
     2821 tacggctgga ggatagtatc tagatgctca acgccagctg ccgagatttg cggtgtgacc
     2881 actgagaagc gcgtaccacc gcgagcaaag gtctggacat attccggata gagcaccgcc
     2941 tttgcctgta cttcattgcg tgcggtaatc agatccagcg tctggatttg cccgatatca
     3001 ataccgagat agcgaattgg catcccgacc gccagttttc cggcatcgaa agcgtgaagc
     3061 gtaatctgcc tgccaaccgc acgggccgct gtttcggaag catacagaat tcgcttgtcg
     3121 cctttacgct gactggcgct ggcaccactg aggttatcga agctaatggc cccctttaat
     3181 gctctggaga gcggggatgc ctgtacggtc aggccactac cattcagctg aactttcgcc
     3241 ccgccttctg cccagaacac gctgttgctg gtcaaaaggt tgcgatattc cggtttaatg
     3301 tgcagatcga tatcgaacgc gttagctcgc ggacgcacgg taataacttc accaacttca
     3361 aatttacggt acagcactac cgatcctgcc tgcacatccg gcagcgtctc tgcactcaaa
     3421 ctcacggttg tggtgggtaa atcgctaagg ctgttctcca gcgctttttc cagattggca
     3481 tacagtggat agctggcttt catctcgcct ttatcgcccg gcagaatacg tatcccgccg
     3541 ttaatccatt ctgaggcgct ggcaccgaga aactcaacgc catccagccc caccttcacg
     3601 tcgacacggc tgttgacgac aaatttgcta tcgcctttta ccagttctcg atgctgaggc
     3661 tcgatggcga cggtaaaggt gacgcctttg ctggtgagtt tacgatcgat aacctggcct
     3721 acctgcacgc cgtgaagaat gagcggctga cccgcatcaa taccgtaact ttccggtgct
     3781 gtcagggtca gtgtcagaac gtccggctct tgcagcaggg ctttttcgcc cggcacaaca
     3841 acgaactctt tgcgtggctc gccatcgccg ggcaccaact caaaggtttt tccggtcagc
     3901 agggcgctga gattagcatc gctaagggat aatttcgggt tgcgtaattc tatgcgggta
     3961 ttttcacgaa gcagggtaac gacgctggga tcaacggtca tttccccagt gactttacca
     4021 ccaggattta aatccagttt agtcagctgt ccgacttcca gcccctgata cattaacggc
     4081 gtcgagtcgg cggttaatcc tgctccactc ggcagttcca gtttcacctt cgcgccactg
     4141 atactgacgt tggcatcaac gccggaaacg ttccagaaac ggctaccttt tttcaccaga
     4201 tcggtaaaac gccgctcgat caggacatca atcaccacgc cttgcttgtt gggattgatg
     4261 gcatagtcgt agacttttcc caccgggatc ttgcggaaat agaccaatga accgctgttc
     4321 agcgaaccga gatcgggggc ttgcaggtgg atcatcagat cgccattgtc cagccgatat
     4381 ttcggttggg tatcgagcgc gacaaagtga tcctgctctt tacctttacc cggcatcatg
     4441 ccgatatagt tcccaccgac gagggcgtcc agcccggaga cacctgccaa cgatgctttt
     4501 ggcgtcatca gccagaactg agtctcttcg cgcagcgcat ctttcatatc ggacttgatg
     4561 ctgaccttga cttcaatctt acgaagatcg tcgctgaggc tgatatcctg cactgttccg
     4621 acttcaacgc cctgataacg aacaggcgta cggcccggaa caataccatc cgccgacata
     4681 aagtcgatgg tgatggtatt accccggtcc tgataactgt cccaaatcag ccaaccggca
     4741 atcattagcg cgatgaaagg cagcagccag aaaggtgaga tacggcgttt atttttaatc
     4801 tgcgcttcag tcgtcgaagc gggcgtttcc tgactcatgt gcatcccaaa gtaagcggct
     4861 gtccagccat tccacagcaa gaatagtcaa aattacggca gcgccaaaat aaaacgcagc
     4921 cggtcccata gtaaaagcga ggatctgatc gcgattaatc agcgacatgg ttaaagatat
     4981 gacaaacagg tcgagcatcg accagcgtcc aatccaggtc accatccgca gtaacagaat
     5041 gcgtgtgcgt aaaccttgct ggcatttaaa atgaatgctc aacagtaaag tgaacatgac
     5101 gatcacttta gtaaacggta ccagaatact ggcgataaac acgattcccg caaccgcaat
     5161 gttgctactt gccagcgaca taattccgga aagaatcgta tcttcctgcc ggccaccatt
     5221 cagataaatg atagaaatag gcaataggtt ggctggtaac aacaaaacga ttgacgctaa
     5281 cagcgccgcc cagcattttt gcaaactatg acgacgacgc aggcgtagcg ggatatggca
     5341 acgcgggcag agaccacgct gatcaggata gccggtaaaa tggcacccaa gacagacacg
     5401 aagtttctcg tccctacgcg tagcggggcg ctgcggataa tatcgctccc acagctcctc
     5461 aacattaaga tgtgacaacg tcaccgtcgt taaaatcacc aacgccacaa aagagaacaa
     5521 gccgacaccc gcctggatat gggcataatc ctgtacctta tatagaagca acgccaatgc
     5581 cgaccaggta gatatccagc attacccact ctttaagtcg ctcaagcatc agcagtaccg
     5641 gacgtaaatt cattcccagt cggttaccaa accataaata tgctatggag gacaccagaa
     5701 tgaggggggc accaataacg cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt
     5761 tggtcatttg ccagatgcct tgcatgacgt tggcgtcaat acgaatgcct aacagccaga
     5821 tatgcaacag cggttcgccc caggcaaacg gcataagcaa cagcatggtg aaggccattg
     5881 ccgccaggcg cgttagcgac cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac
     5941 aataggcact ttgatgagaa tttatctcgg gcaggctaaa cagcatgtca cattgcgggc
     6001 aacgttggta atcaccacgc ggcagttcct cgccgattgc cctcactgtt atcttttttg
     6061 tcggcgtaat ttgtggtgtg ttaagagcca tgacgtgcca aatgtaataa gaattaacat
     6121 taatcttaac ccatgatggg aatgatcttg agcattaacg catttaatgc ttattttaat
     6181 aggctggcag ttccaggtaa gacaaccaag tgattaaata atgaacaaaa cagaatttta
     6241 cgcggattta aatcgcgact ttaacgcgct gatggcggga gaaaccagtt ttctggcaac
     6301 gcttgcgaac accagtgcgt tgttatacga gcgtctcact gacgttaact gggcaggttt
     6361 ttatttgctt gaggacgata cactggtact cggaccattt cagggcaaaa ttgcctgtgt
     6421 ccggataccc gtcgggcgcg gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg
     6481 tatcgaggat gttcacgcgt ttgacgggca tattgcctgt gatgcggcga gtaattctga
     6541 aattgttctg ccgctggtgg tgaaaaatca gattattggt gttctcgaca tcgatagtac
     6601 cgtcttcgct cgctttacag acgaggacga gcaaggctta cgtcagcttg tggcacagct
     6661 tgaaaaagtg cttgcaacga cggattacaa aaaattcttt gcgagcgtcg caggataatc
     6721 aacggataac gtagcattta ctggtagcgt cattataatg acgcctgttc atgcctgcgc
     6781 ttgttggcta cgtccgttgt aatcaggaaa tttcatggaa aatcaaccta agttgaatag
     6841 cagtaaagaa gtaatcgcgt ttctcgccga acgttttccc cactgtttca gtgcggaagg
     6901 tgaagcgcgt ccgctgaaaa ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga
     6961 aatgaacctg agcaaaacgc aattacgatc cgctttacgt ctctacactt cgagctggcg
     7021 ttatctttac ggtgttaaac ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg
     7081 tgagctggac gagcaacatg tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg
     7141 tgttcaggca cagcgtgctg aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg
     7201 tgagaaagaa gacgcaccgc gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa
     7261 agaaggcgct gaacgtaaac ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt
     7321 aaaagcacct cgcgaagaac agcacacccc ggtttctgac atttcagctc tgactgtcgg
     7381 acaagccctg aaggtgaaag cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac
     7441 caaagacggc gtccgcgtcc agctgaattc gggtatgtct ttgattgtgc gcgcagaaca
     7501 cctggtgttc tgaaacggag gccgggccag gcatgaacat gttttttagg cttaccgcgt
     7561 tagctggcct gcttgcaata gcaggccaga ccttcgctgt agaagatatc acgcgtgctg
     7621 atcaaattcc ggtattaaag gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt
     7681 cgcgcttcac ccgttctcat tatcgccagt tcgacctcga tcaggcattt tcggccaaaa
     7741 tctttgaccg ctacctgaat ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg
     7801 ttgaacagtt cgcgaaaaag aaaaccgagt taggcgatga actgcgttca ggcaaactcg
     7861 acgttttcta cgatctctac aatctggcgc aaaagcgccg ttttgaacgt taccagtacg
     7921 ctttgtcggt actggaaaag ccgatggatt tcaccggcaa cgacacttat aaccttgacc
     7981 gcagcaaagc gccctggccg aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag
     8041 tcaaattcga cgagttaagc ctgaagctga caggaaaaac ggataaagaa attcgtgaaa
     8101 ccctgacacg ccgctacaaa tttgccattc gtcgtctggc gcaaaccaac agcgaagatg
     8161 ttttctcgct ggcaatgatg gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt
     8221 ccccgcgtaa taccgaacag ttcaacactg aaatgagttt gtcactggaa ggtattggcg
     8281 cagtgctgca aatggatgat gactacaccg ttatcaattc gatggtggca ggtggtccgg
     8341 cagcgaagag taaagctatc agcgttggtg acaaaattgt cggtgttggt caaacaggca
     8401 agccgatggt tgacgtgatt ggctggcgtc ttgatgatgt ggttgcctta attaaagggc
     8461 cgaagggcag taaagttcgt ctggaaattt tacctgctgg taaagggacc aagacccgta
     8521 ctgtaacgtt gacccgtgaa cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga
     8581 agaccgtcgg taaagagaaa gtcggcgtgc tggatattcc gggcttctat gtgggtttga
     8641 cagacgatgt caaagttcaa ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca
     8701 tcgacctgcg tagcaatggc ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt
     8761 ttattcctgc gggtcccatt gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca
     8821 gcgataccga cgggcaggtg ttctataaag gcccactggt ggtactggtt gaccgtttca
     8881 gtgcttcggc ttcagaaatc tttgccgcgg caatgcagga ttacggtcgt gcgctggttg
     8941 tgggtgaacc gacgtttggt aaaggcaccg ttcagcaata ccgttcattg aaccgtattt
     9001 acgatcagat gttacgtcct gaatggccag cgctgggttc tgtgcagtac acgatccaga
     9061 aattctatcg cgttaacggc ggcagtacgc aacgtaaagg cgtaacacca gacatcatca
     9121 tgccgacggg taatgaagaa acggaaacgg gtgagaaatt cgaagataac gcgctgccgt
     9181 gggatagcat cgatgccgcg acttatgtga aatcaggaga tttaactgcc tttgaaccgg
     9241 agctgctgaa ggaacataat gcgcgtatcg cgaaagatcc tgagttccag aacatcatga
     9301 aggatatcgc gcgcttcaac gctatgaagg acaagcgcaa tatcgtttct ctgaactacg
     9361 ctgtgcgtga gaaagagaat aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac
     9421 gctttaaacg cgaaggtaaa ccggagttga agaagctgga tgatctaccg aaagattacc
     9481 aggagccgga tccttatctg gatgagacgg tgaatatcgc actcgatctg gcgaagcttg
     9541 aaaaagccag acccgcggaa caacccgctc ccgtcaagta atatcaatca ggcacaagaa
     9601 attgtgcctg attttttaac agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa
     9661 gttgtgtctt tctggtgact tacgcactat ccagacttga aaatagtcgc gtaacccata
     9721 cgatgtgggt atcgcatatt gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg
     9781 aatcgcgctc ttcctgctaa cgaacctggc cgtaatggtc gttttcgggc tggtactgag
     9841 cctgacaggg atacagtcga gcagcgttca ggggctgatg atcatggcct tgctgttcgg
     9901 ttttggtggt tccttcgttt cgcttctgat gtccaaatgg atggcattac gatctgttgg
     9961 cggggaagtg atcgagcaac cgcgtaacga aagggaacgt tggctggtca atactgtagc
    10021 aacccaggct cgtcaggcgg ggatcgctat gccgcaagtg gctatctacc atgcgccgga
    10081 catcaacgct tttgcaaccg gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg
    10141 tttgctgcag aacatgagcc cggatgaagc cgaggcggta attgctcacg aaatcagcca
    10201 catcgccaat ggtgatatgg tcaccatgac gctgattcag ggcgtggtga acaccttcgt
    10261 tatctttatt tcccgtattc tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga
    10321 tgaaggtgaa gagagcaacg gcaacccgct gatctacttt gcggttgcaa cggttctgga
    10381 actggtgttt ggtattctgg cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt
    10441 ccatgctgat gccggttcgg caaaactggt tggtcgcgag aaaatgattg ccgcgctgca
    10501 gcgcctgaaa accagctatg aaccgcaaga agcaaccagc atgatggctt tctgcattaa
    10561 cggtaagtcg aaatcgctca gtgagttgtt catgactcac ccgccgctgg ataaacgtat
    10621 tgaagctctg cgtacgggtg aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt
    10681 cctgatcgac gcgctttttt tatgccctgg ctcgtggctg agtgatacgt aaaccactga
    10741 cacaggcagc aatcactgcc agaatagccg cagccatcag cgagacgtgc gtaccattat
    10801 caccaaactg atttagcatc agcgccacca gcgccgcgcc actactctga cccagtagac
    10861 gagccgttcc taacatgcca ctggccccac cgctacgttc gcgcggagcg gaggtaataa
    10921 tggtgtggtt attgggtgac tggaataagc caaatccagc gccacataag atcatcggcc
    10981 agataatatt gatatccgca ggtgacgcgg gcagcagaac cagggaaaaa agccccgcag
    11041 ccatgatgaa caaccctaaa gcacccagca atcctgcatg tacgcgttca atcaaatagc
    11101 ctgccagcgg agccatcacc atcgttgcta acggccacgg tgtcagaagt aaacctgttt
    11161 cgacttcact acgcccgagc acggtttgca ggtaaaaggg cagggaaacc attgccagca
    11221 tttgtgcgca gaaagagcaa acagatgtgc aaatagaaag tgaaaacagc gggatacgca
    11281 gtaaatccac cggtagcagc ggtacgggaa gagaaagctg gcggcgaata aagaaaatac
    11341 caacaacaac cattaccacc agttccgcag caattaacgt cagcgattgc ccctgagcga
    11401 aaccactcaa cgcagtgata agcaggccga aggttaacgc gttcatcacg gcgctgggca
    11461 ggtcgaaacg gggtttactg gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa
    11521 gcagggcgat aatacctaac ggtacgttga ttaaaaataa ccatttccag gatgcgatgg
    11581 agaggattgc tgcagcaatt gtcggcccgg cagcagaaga gacggcaaca ataaacgagt
    11641 ttatgcccat ccctctcccc agaaaacgtt gtggatagat caggcggata agtgcggtat
    11701 taacgctcat caacgctgca ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt
    11761 gcagcgaatc agaaagggcg cagaacagtg aagacaacag aaaaacgacc agaccgcatt
    11821 tataaatacg tcgatagcca aacatatcgc ccagaaacga aaacgagagc agggagatga
    11881 caatggcgat ttgataggcg ttcactaccc agatggaact ggctggtgtg gcatgaaggt
    11941 ccgtggcgat tgttggcagg gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa
    12001 taccaatcac aatggttaat atcgcaccgt atcgctgggg caatggcagg ccgtcggcct
    12061 gaacttttgg cataggaatt ttatatcttt ggtgaataat caacaggggt atgattctag
    12121 cactgatatt cacggtggat gtcgcagaat tgtaactaac cggcaaaaga tggattgcga
    12181 ccaccctggt gcgaaattat aataaaaaac agttctgatt tttataaaac actcgcaatg
    12241 aggtgataaa tggctaacgc agatctggat aaacagcctg attctgtatc ttccgtgcta
    12301 aaagtttttg gcattttgca ggcgctgggt gaagagcgcg aaatagggat aaccgagctg
    12361 tcgcagcgcg tcatgatgtc aaaaagcacc gtttatcgct ttttacagac catgaaaacc
    12421 ttaggttatg tggcgcagga aggggagtcg gagaaatatt ccctgaccct gaaattgttt
    12481 gaactgggcg ctcgcgcgtt acaaaacgtc gatttaattc gtagcgcaga tatccagatg
    12541 cgtgagatct cccgcctgac caaagaaact atccacctcg gcgcactgga cgaagacagt
    12601 attgtttaca ttcacaaaat tgactctatg tacaatttgc gcatgtattc acggattggg
    12661 cgtcgtaatc cgctgtacag caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc
    12721 gatgaagtga agcaaattct tgagggcgtg gagtataaac gcagtaccga gcggaccatc
    12781 accagtacag aagcgttatt acccgttctg gaccaggtgc gcgagcaggg gtatggcgaa
    12841 gataacgaag agcaggaaga agggctgcga tgcattgcgg taccggtatt tgatcgcttt
    12901 ggcgtggtca ttgccggttt gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt
    12961 ttacaggaat atgtcgcaat gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt
    13021 tatcacgact atccgttctg atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt
    13081 ctttatttag catgagaaaa ataaagttga aggtgcgtta tattaaacgc gcttgctata
    13141 agaatatttt actcatgagt gagaatctgg ttatttatta cccttaacca ttatcgacca
    13201 cgatattgct tttacgtaac agtgggcaat ctgttatccc caagaaacca cttttagtgt
    13261 gcaagtattg taccgtgctg gtgcctctgg ccgtcagata ggtacattgc aaacctaatc
    13321 ctgcggcatt ctctttgctt ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc
    13381 ataccagtgc tatcagcata actgtgcgaa tgatgaatcg cattacaatc tcttctcttt
    13441 ttatgttcgc ttaatcgtag cggcaatatg cgctgaagca agctactcat tccgaaaaag
    13501 cacgaatatc gacatagtta ggcgctgttt aactaacgca tgctagttta atgacataag
    13561 gtaggtgaag cggagattgg agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg
    13621 ttggcttgct tgctgctttg ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa
    13681 tttttcagcg gaatttgtgc cattaaccag tttatcccgt ggtgatatca ttttttcagc
    13741 actagatttt cttcctctcc agcagtggta gaatagcgcc tctactcaga ggtggtgaaa
    13801 tgaacgaagt tgtaaattca ggcgtgatga acattgcgtc tttggttgta tcggtggtgg
    13861 ttcttcttat cgggctcatc ttgtggtttt ttatcaatcg tgccagttca cggactaacg
    13921 aacagattga actgcttgag gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac
    13981 gtcgtttgtg cgaagcaaac gaaccagaga aagcagataa aaagaccatt gagagtcaaa
    14041 aatcggttga tgacgaagat attattcgcc tggtcgccga acgataatcc ttcctcaatc
    14101 ttgagtaacc ccgtcgttga agggttactc aattcatcct gattatacgc actgtcatgg
    14161 tgcaactgct tcaaattaaa actctggtct gacttcaaat aattatcata ttatttattt
    14221 gcgtaacggg atatcagaaa aagatatttt cctcattaat ttattaggat gtttacatca
    14281 gatttgtgat taagcgtggt attatttatt agcgaaacgt ttctctcttg atttttttgc
    14341 tcactcatca atttttctta ttttaaattt acaatccctt tggggattga cttctcttta
    14401 gggtaattga tagccgttaa ctgactgttt tatgagaaaa agtgatataa ctttttattc
    14461 attgcatagc agaaaatgtg atattgcacg cactatgtaa taacttctcc ctctggcctg
    14521 gaacaactga acttattgaa ctatgttaga aactacgcca gtttaagtat ctgcctgaac
    14581 tggcaaggtt aagcacaatg atatatcggc gcgtattccg ttgcataagt gtgcaaaaaa
    14641 agtggaagac gtatcgagat ttgtgcgtct gatcgagaca tgtttaaaaa tggcttgcca
    14701 taattaacgt tgtatgtgat aaaagatttc gggttaaacg aggtacagtt ctgtttatgt
    14761 gtggcatttt cagtaaagaa gtcctaggta aacacgttga cgttgaatac cgcttctctg
    14821 ccgagcctta tattggtgcc tcatgcagta atgtgtcagt tttatctatg ttatgcctgc
    14881 gggcgaagaa aacaatctaa ggaatttttc aaatggcaaa gattaaaggt caggttaagt
    14941 ggttcaacga gtctaaaggt tttggcttca ttactccggc tgatggcagc aaagatgtgt
    15001 tcgtacactt ctccgctatc cagggtaatg gcttcaaaac tctggctgaa ggtcagaacg
    15061 ttgagttcga aattcaggac ggccagaaag gtccggcagc tgttaacgta acagctatct
    15121 gatcgaatcc actgatctga agtgtgaata cgcttcaatc tcgctataaa gcctcgtcga
    15181 atgcgaggct ttttactatg ctttatcttc gctcctggcg ttcggatatt tgccagccac
    15241 gtgattcgcg ttacacttgc ggcctttagt atcctgccgg agttgtcatg tcgttttcct
    15301 gtccactttg ccatcagcct ctttcgcgtg aaaaaaacag ctatatctgt ccccaacgac
    15361 atcagtttga tatggcgaaa gaagggtatg tcaatctgct gcccgttcag cataaacggt
    15421 ctcgtgatcc gggcgacagc gcggaaatga tgcaagcacg ccgcgcattc ttagatgccg
    15481 gacattatca gccgctgcgt gatgcaattg tcgcccaact gcgggaacgg cttgatgaaa
    15541 aggccacggc ggtgctggat attggctgtg gtgaagggta ttacacacac gcatttgccg
    15601 atgcgttgcc cgaaatcacc acgtttggcc tggatgtctc gaaggtagcg ataaaagcgg
    15661 cggcgaaacg ctatccgcag gtcactcttt gtgtcgcttc cagccaccgt ttgccgtttt
    15721 ccgataccag tatggacgcc ataatacgta tttacgcgcc gtgtaaagca gaagaattag
    15781 cacgagtagt gaagcccggc ggctgggtca ttactgccac gccgggaccg cgacatttga
    15841 tggagctgaa ggggctgatt tacaatgaag tacatcttca tgcacctcat gcagaacaac
    15901 tggaaggttt tacattacag cagagtgcgg agttgtgtta tccgatgcgt cttcgcggtg
    15961 atgaagccgt cgcattattg cagatgacgc cgtttgcctg gcgtgcgaag ccagaagtct
    16021 ggcaaacact ggcagcaaaa gaagtgttcg actgccagac ggactttaat attcacctct
    16081 ggcagcgttc ttattaaccg tggaagtgcg tccagaggat ctggacgccg atgccgatca
    16141 gcaccagccc gccgagaatt tccgcttttt tcccaataat tgagccgata aagcgaccaa
    16201 ccatcatccc taatgtcgac ataatcaagg ttgcacaacc aatggccaat gcggtcgcga
    16261 taatgttgac ctgaaggaaa gcaagaccaa cacccacagc catggcatcc aggctggtgg
    16321 caatcgcggt ggttaccagt agccagaaac cgtgccgacg gcgcggctct tcatcttcat
    16381 catctgagcc acgaaaaccc tcaataatca ttcgcccgcc gaggaatatc agcagcacaa
    16441 acgcaatcca gtgattccat tcaaggacaa accggctggc taacatgccc attccccagc
    16501 cgatcagcgg cgtcagggtt tcgacggcac caaaaataag gccggttcgc aatgcttcag
    16561 aaaatttcgg tttatggagg ggggcacctt taccgattga tgcagcaaat gcatccatcg
    16621 acataccaaa cgcaagaaga acagtagcag tgatattcat gacaacgtcc tgaccggggt
    16681 atccataaaa cacatcatcg cccccagcaa acatgcatac atgaacaaca agccgcgatg
    16741 ggttttcgcg ttcatgcagg catagcgatt gatgtgcata tggtctcgcc tgactgaaag
    16801 tcttcagtcc gtacgtgcca cgttttgcaa cgagtatgtt gacacgtaca tttccggaat
    16861 ctggaaatcg gctactcccc aaatgagggc gcaaccttaa catattttga gaatataaaa
    16921 caactgttgg aatatattat ttcattgctt tattgataac gattttcatt tatgtttata
    16981 tatcaatgaa acgttaaaat aaataacaaa taagggaaga gataatatag cctaagctat
    17041 gtctgattat taaaaataca ctaaacatag ctttggctaa attcataagt tattgagttg
    17101 ata
//
LOCUS       DADMGC010000084        16813 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.084, whole genome shotgun sequence.
ACCESSION   DADMGC010000084 DADMGC010000000
VERSION     DADMGC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16813
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.084"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..942
                     /locus_tag="JNZ73_002708"
     CDS             <1..942
                     /locus_tag="JNZ73_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HAY8732938.1"
                     /translation="TGLGTITAANTVTVRSRVNGQLMALHFQEGQQVKAGDLLAEIDP
                     SQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGT
                     IKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDL
                     VFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKA
                     RFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLV
                     TPGIQDSQKVEVVEAQSATTPEEKATSREYAKKGARS"
     gene            942..4091
                     /gene="mdtB"
                     /locus_tag="JNZ73_002709"
     CDS             942..4091
                     /gene="mdtB"
                     /locus_tag="JNZ73_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HAY8732939.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLNVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVALSTLLLSVLLWVFI
                     PKGFFPVQDNGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTN
                     PSLNSARLQINLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRT
                     QYQFTLQATSLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLG
                     ISMADVDNALYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGV
                     VPLSSIAKIEQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVD
                     ITTQFQGSTLAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGA
                     LLALMIAGSELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLL
                     RFRPILMTTLAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLL
                     FDRLVLWTKSRFARHEEEA"
     gene            4092..7157
                     /gene="mdtC"
                     /locus_tag="JNZ73_002710"
     CDS             4092..7157
                     /gene="mdtC"
                     /locus_tag="JNZ73_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HAY8732940.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLRITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            7158..8573
                     /locus_tag="JNZ73_002711"
     CDS             7158..8573
                     /locus_tag="JNZ73_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8732941.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            8570..9973
                     /gene="baeS"
                     /locus_tag="JNZ73_002712"
     CDS             8570..9973
                     /gene="baeS"
                     /locus_tag="JNZ73_002712"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HAY8732942.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLIDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            9970..10692
                     /gene="baeR"
                     /locus_tag="JNZ73_002713"
     CDS             9970..10692
                     /gene="baeR"
                     /locus_tag="JNZ73_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HAY8732943.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            10883..11215
                     /locus_tag="JNZ73_002714"
     CDS             10883..11215
                     /locus_tag="JNZ73_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAY8732944.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            11362..12723
                     /locus_tag="JNZ73_002715"
     CDS             11362..12723
                     /locus_tag="JNZ73_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HAY8732945.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(13065..13382)
                     /locus_tag="JNZ73_002716"
     CDS             complement(13065..13382)
                     /locus_tag="JNZ73_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732946.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            13788..14687
                     /gene="yegS"
                     /locus_tag="JNZ73_002717"
     CDS             13788..14687
                     /gene="yegS"
                     /locus_tag="JNZ73_002717"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HAY8732947.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(14769..15548)
                     /locus_tag="JNZ73_002718"
     CDS             complement(14769..15548)
                     /locus_tag="JNZ73_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY8732948.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(15648..16688)
                     /gene="gatD"
                     /locus_tag="JNZ73_002719"
     CDS             complement(15648..16688)
                     /gene="gatD"
                     /locus_tag="JNZ73_002719"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY8732949.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLDPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(16736..>16813)
                     /locus_tag="JNZ73_002720"
     CDS             complement(16736..>16813)
                     /locus_tag="JNZ73_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY8732950.1"
                     /translation="YLTGIFMTWRRARGFTKQEKAVLAE"
BASE COUNT         3889 a         4500 c         4407 g         4017 t
ORIGIN      
        1 accgggcttg gcaccattac cgccgctaat actgttacgg tgcgcagccg cgtgaacggt
       61 caactgatgg cgttacattt ccaggaaggc cagcaggtca aagcaggcga tttactggca
      121 gaaattgacc ccagccagtt caaagttgca ttagcacaaa cccagggcca actggcaaaa
      181 gataaagcca cgcttgctaa cgcccgccgt gatctggcgc gttatcagca actggcaaaa
      241 accaatctcg tctcccgtca ggagctggat gcccaacagg cgctggtcag tgaaaccgaa
      301 ggcaccatta aggctgatga agcaagcgtc gccagcgcgc agttgcaact cgactggagc
      361 cgcatcaccg caccagtcga tggtcgcgtt ggtctcaagc aagttgatgt tggtaaccaa
      421 atctccagtg gtgataccac cggaattgtg gtgatcaccc agacgcatcc tatcgattta
      481 gtctttaccc tgccggaaag cgatatcgct actgtagtgc aggcgcaaaa agccggaaaa
      541 ccgctgatgg tagaagcctg ggatcgcacc aattcgaaga aattaagtga aggcacgctg
      601 ttaagcctcg ataaccaaat cgatgccaca accggtacga ttaaagtgaa agcacgcttt
      661 aataatcagg atgatgcgct gtttcccaat cagtttgtta acgcgcgcat gttagtcgac
      721 accgaacaaa acgccgtagt gatcccaaca gccgccctgc aaatgggcaa tgaaggccat
      781 tttgtctggg tgctgaatag cgaaaacaag gtcagcaaac atctggtgac gccgggcatt
      841 caggacagtc agaaagtgga agtggtggaa gcccagagcg ccaccactcc ggaagagaaa
      901 gccaccagcc gcgaatacgc gaaaaaagga gcacgctcct gatgcaggtg ttacccccga
      961 gcagcacagg cggcccgtcg cgcctgttta ttatgcgtcc tgtcgccacc acgctgctga
     1021 tggtggcgat cttactcgcc gggattatcg gttatcgcgc cctgcccgtt tcggcgctgc
     1081 cggaagtgga ctatccgacc attcaggtgg tcacgctcta cccaggtgcc agcccggatg
     1141 tcatgacctc tgccgttacc gcgccgctcg aacgccagtt cgggcagatg tctggcctga
     1201 aacagatgtc gtcgcaaagt tccggcggtg cgtcagttat cactttgcag ttccagctaa
     1261 cattaccgct caatgtcgcc gagcaggaag tgcaggccgc gattaacgct gcgaccaact
     1321 tgttgccgag cgatctgcct aacccgccgg tttacagcaa agtgaacccg gcagatccgc
     1381 cgatcatgac gctcgccgtc acctcaaccg ccatgccgat gacgcaagtg gaagatatgg
     1441 tggaaacccg cgtcgcgcag aaaatctcgc agatttccgg cgtcggcctg gtgacgcttt
     1501 ccggcggtca gcgtccggct gttcgcgtca aacttaacgc tcaggcgatt gccgccctcg
     1561 gcctgaccag cgaaaccgtg cgcaccgcca ttaccggcgc taacgttaac tcggcaaaag
     1621 gtagcctcga cggcccttcc cgtgcggtca cgctttccgc gaacgaccag atgcaatccg
     1681 ccgaagagta tcgccagcta atcatcgcct accagaacgg cgcgccaatt cgtctgggcg
     1741 atgtagcaac tgtagagcaa ggtgcagaaa acagctggct cggcgcgtgg gcgaacaaag
     1801 aacaggccat tgtgatgaat gttcagcgcc agcccggtgc taacattatc tccaccgccg
     1861 acagcattcg gcagatgctg ccacagctca ctgagagtct gccgaaatcg gtgaaggtga
     1921 cagtgctttc cgatcgcacc accaatatcc gcgcatccgt cgatgatact cagtttgaat
     1981 tgatgatggc tatcgcgctg gtagtcatga ttatctacct gtttttgcgc aatattccgg
     2041 cgaccatcat tcccggtgtt gctgtaccgc tgtcgttaat cggcactttc gcggttatgg
     2101 tgtttctcga tttttcaatc aataacctga cactgatggc gttaactatc gccaccggat
     2161 tcgtggtcga tgacgccatc gtggtgatcg aaaacatttc ccgctatatc gaaaaaggcg
     2221 aaaaaccgtt ggcggcggcg ctcaagggcg caggtgaaat cggctttacc attatctcgc
     2281 tgaccttctc actgattgcg gtgttgatcc cactgctgtt tatgggcgat atcgtcgggc
     2341 gactgttccg cgaatttgct attaccctgg cggtagcgat tttgatctca gcggtggtgt
     2401 cgctgaccct gacaccgatg atgtgcgcgc ggatgctcag ccaggagtcg ttgcgtaaac
     2461 agaaccgctt ctcccgtgcc tcggaaaaaa tgttcgacag gataatcgcc gcctatggtc
     2521 gtggactggc gaaagtgctg aatcatccgt ggctgacctt aagcgtggca ctcagcacgc
     2581 tgctgcttag cgtggcactc agcacgctgc tgcttagcgt gctgctgtgg gtgttcattc
     2641 cgaaaggttt cttcccggta caggacaacg gcattattca gggcactttg caggcaccgc
     2701 aatccagctc ctttgccaat atggcccagc gacaacgcca ggtcgcggac gtgattttgc
     2761 aggatccggc agtgcaaagc ctgacctcat ttgttggcgt tgatggcact aacccgtcgc
     2821 tgaatagtgc acgtttacaa atcaacctca aaccgttgga tgaacgtgat gatcgggtgc
     2881 aaaaagtcat cgcccgtctg caaacggcgg tagataaagt gccgggcgtc gatctcttcc
     2941 tgcaaccaac gcaggacctg accatcgata ctcaggtcag ccgcacccag taccagttta
     3001 ccttgcaggc cacgtcactg gatgcgctca gtacctgggt gccacagttg atggaaaaac
     3061 tccagcaact gccacagctt tctgatgtct ccagcgactg gcaggacaaa gggctggtgg
     3121 cgtatgtcaa tgttgatcgc gacagcgcca gccgtctggg gatcagcatg gcggacgtcg
     3181 ataacgccct gtacaacgcg tttggtcagc gactgatttc cactatttat actcaggcta
     3241 accagtatcg cgtggtgctg gagcacaata ccgaaaatac cccaggcctc gcggcgctgg
     3301 ataccattcg cctgaccagc agcgacggtg gcgtggtgcc gttaagctca attgccaaaa
     3361 ttgagcagcg ttttgcgccg ctttccatca accatctgga tcagttcccg gtaacaacca
     3421 tctcctttaa cgtgccggat aactattcgc tgggcgatgc ggtgcaggcg attatggaca
     3481 ccgaaaagac gctgaatctg ccggtggata tcaccacgca attccaaggc agcaccctcg
     3541 ccttccagtc ggcgctgggc agcactgtct ggctgattgt cgcggcggtg gtggcgatgt
     3601 atatcgtgct cggcattctg tacgagagct ttattcaccc gatcaccatt ctctcgacgc
     3661 tacccaccgc aggggtcggc gcgctgctgg cattaatgat tgccggtagc gaactggatg
     3721 tgattgcgat tatcggcatt attttgctga tcggtattgt gaagaagaac gccatcatga
     3781 tgatcgactt cgcgctggct gctgagcgcg agcaaggcat gtcgccgcgc gatgcaatct
     3841 accaggcttg tctgttgcgt tttcgtccga tcctgatgac caccctggcg gctctgcttg
     3901 gcgcgctgcc gctgatgttg agtaccgggg tcggcgcgga actgcgtcgt ccgttaggta
     3961 tcggcatggt cggcggtctg attgtcagcc aggtgctgac gctgttcacc acgccggtga
     4021 tttatctgct gttcgaccgc ctggtattgt ggaccaaaag tcgctttgct cgccatgaag
     4081 aggaggcgta agtgaagttt tttgccctct tcatttaccg cccggtggcg acgattttac
     4141 tgtcggtcgc cattaccctg tgcggcatac tgggtttccg tatgctgccg gtcgccccgc
     4201 tgccgcaggt cgattttccg gtgattatgg tcagcgcctc gctgcccggt gcgtcgccgg
     4261 agacaatggc gtcttccgtt gccacgccgc tggagcgctc acttgggcgc attgccgggg
     4321 ttagtgaaat gacctccagc agttcgctcg gcagcacgcg cattattttg cagtttgatt
     4381 ttgaccggga tatcaacggc gcagcgcgtg atgtgcaggc ggcgatcaac gctgcacaaa
     4441 gtttgctgcc cagcgggatg cccagccgcc cgacctatcg caaagcgaac ccgtcggatg
     4501 cgccaattat gatcctcacg ctgacatccg atacttattc gcagggtgaa ctgtacgatt
     4561 tcgcctcgac gcagctggct ccgacgattt cgcaaatcga cggtgtcggt gatgtcgatg
     4621 tcggtggcag ctcgctgccc gccgtgcgcg tcgggctgaa cccgcaggcg ctgtttaatc
     4681 agggcgtgtc gctggacgac gtacgcactg ccatcagcaa tgccaacgtg cgtaaaccgc
     4741 agggggcgct ggaagatggc actcaccgct ggcagatcca gaccaacgat gaactgaaaa
     4801 ccgccgctga atatcagccg ctgattattc actacaacaa cggcggcgcg gttcgtctgg
     4861 gcgatgtggc gacggtgact gactcagtgc aggatgtgcg caacgccggg atgaccaacg
     4921 ccaaaccggc tattttactg atgatccgca aactgccgga agccaatatt atccagacgg
     4981 tagacagcat ccgggcaaaa ttaccagagt tgcaggagac cattccggcg gcgattgatc
     5041 tgcaaattgc ccaggatcgc tcccccacca ttcgcgcctc gctggaagaa gtcgagcaaa
     5101 cgctgattat ctcggtggcg ctggtgattc tggtggtctt tttattcctg cgctcgggtc
     5161 gcgccactat tattcccgcc gttgcggtgc cggtttcgct gattggtacg tttgcggcga
     5221 tgtacctgtg cggattcagt ctcaataatc tttcgctgat ggcgctcacc attgctactg
     5281 gtttcgtggt ggatgacgcc attgtggtgc tggaaaacat tgcccgtcat ctggaagcgg
     5341 ggatgaaacc actacaggcc gcgctgcaag gtactcgcga agtcggcttt acggtgctgt
     5401 cgatgagtct gtcactggtg gcggtgttcc tgccgctgct gttgatgggc ggattgccgg
     5461 gccgactgtt acgcgagttt gccgtgacgc tttctgtcgc cattggtatt tcgttgctgg
     5521 tttccctgac gctaacgcca atgatgtgcg gctggatgct aaaagccagc aagccgcgtg
     5581 agcaaaagcg actgcgtggt tttggccgca tgttggtagc cctgcaacaa ggctacggca
     5641 agtcgctgaa atgggtgctc aatcataccc gtctggtggg cgtggtgctg cttggcacca
     5701 ttgcgctgaa tatctcgatc ccgaaaacct tcttcccgga gcaggacacc ggcgtactga
     5761 tgggtgggat acaggcggat cagagtattt cgtttcaggc gatgcgcggc aaattgcagg
     5821 atttcatgaa aattatccgt gacgatccgg cagtggataa tgtcaccggc tttaccggcg
     5881 gttcacgagt taacagtggg atgatgttta tcaccctcaa gccacgcgac gaacgcagcg
     5941 aaacggcgca gcaaattatc gaccgtctgc gcgtgaagct ggcgaaagaa ccgggggcga
     6001 atctgttcct gatggcggta caagatattc gcgttggtgg gcgtcagtcg aacgccagct
     6061 accagtacac gttgttatcc gacgacctgg cggcactgcg cgaatgggag ccgaaaatcc
     6121 gcaaaaaact ggcgacgttg ccggaactgg cggacgtgaa ctccgatcag caggataacg
     6181 gcgcggagat gaatctggtt tacgaccgcg acaccatggc acggctggga atcgacgtac
     6241 aggccgccaa cagtctgtta aataacgcct tcggtcagcg gcaaatctcg accatttacc
     6301 agccgatgaa ccagtataaa gtggtgatgg aagtggatcc gcgctatacc caggacatca
     6361 gtgcgctgga aaaaatgttc gttatcaata acgaaggcaa agcgatcccg ctgtcgtatt
     6421 tcgctaaatg gcaaccggcg aatgccccac tatcggtgaa tcatcaggga ttatcggcgg
     6481 cctcgaccat ctcgttcaac ctgccgaccg gaaaatcgct ctcggacgcc agtgcggcga
     6541 tcgatcgcgc aatgacccag cttggtgtgc cttcgacggt gcgcggcagt tttgccggca
     6601 cggcgcaggt tttccaggag acgatgaact cgcaggtgat cctgattatc gccgccatcg
     6661 ccacggtgta tatcgtgctg ggtatccttt acgagagtta cgtacatccg ctgacgattc
     6721 tctccaccct gccctcggcg ggcgttggag cgctgttggc gctggagctg ttcaatgccc
     6781 cgttcagcct aatcgccctg atagggatca tgctattaat cggcatcgtg aagaaaaacg
     6841 ccattatgat ggtcgatttt gcgcttgaag cccaacggca cggtaacctg acgccgcagg
     6901 aagctatttt ccaggcctgt ctgctgcgtt ttcgcccgat tatgatgacc accctggcgg
     6961 cgctgtttgg cgcgctgccg ctggtattgt cgggcggtga cggctcggag ctgcggcaac
     7021 ccctgaggat caccattgtc ggcggtctgg tgatgagcca gctcctgacg ctgtatacca
     7081 cgccggtggt gtatctcttt ttcgaccgtc tgcggctgcg tttttcgcgt aaacctaaac
     7141 aaacggtaac cgagtaaatg acagatcttc ccgacagcac ccgctggcaa ttatggattg
     7201 tggctttcgg cttctttatg cagtcgctgg acaccaccat cgtaaacacc gcccttccct
     7261 caatggcgca aagcctcggg gaaagtccgt tgcatatgca catggtcatt gtctcttatg
     7321 tgctgaccgt ggcggtgatg ctgcccgcca gcggctggct ggcggacaaa gtcggcgtgc
     7381 gcaatatttt ctttaccgcc atcgtgctgt ttactctcgg ttcactgttt tgcgcgcttt
     7441 ccggcacgct gaacgaactg ttgctggcac gcgcgttaca gggcgttggc ggtgcgatga
     7501 tggtgccggt cggcaggttg acggtgatga aaatcgtacc gcgcgagcaa tatatggcgg
     7561 cgatgacctt tgtcacgtta cccggtcagg tcggtccgct gctcggtccg gcgctcggcg
     7621 gtctgctggt agaatacgcg tcgtggcact ggatcttttt gatcaacatt ccggtgggga
     7681 ttatcggtgc gatcgccaca ttgatgttaa tgccgaacta caccatgcag acgcggcgct
     7741 ttgatctttc cggcttttta ttgctggcgg ttggcatggc ggtattaacc ctggcgctgg
     7801 acggcagtaa aggtacaggt ttatcgccgc tggcgattgc tggcctggtc gcagttggcg
     7861 tggtggcact ggtgctttat ctgctgcacg ccagaaataa caaccgcgcc ctgttcagtc
     7921 tgaaactgtt ccgcactcgt accttttcgc tgggcctggc ggggagcttt gccggacgta
     7981 ttggcagtgg catgttgccc tttatgacac cggttttcct gcaaattggc ctcggtttct
     8041 cgccgtttca tgccggactg atgatgatcc cgatggtgct tggcagcatg ggaatgaagc
     8101 gaattgtggt acaggtggtg aatcgctttg gttatcgtcg ggtactggta gcgaccacgc
     8161 tgggtctgtc gctggtcacc ctgttgttta tgactaccgc tctgttgggc tggtactacg
     8221 ttttgccgtt cgtcctgttt ttacaaggga tggtcaactc gacccgtttc tcctccatga
     8281 acaccctgac gctgaaagat ctcccggaca atctggcgag cagcggaaac agcctgctgt
     8341 cgatgattat gcaattgtcg atgagtatcg gcgtcaccat cgccgggctg ttgctggggc
     8401 tttttggttc gcagcatgtc agcgtcgaca gcggcaccac acaaaccgtc tttatgtaca
     8461 cctggcttag catggcgttt atcatcgccc ttccggcgtt catctttgcc agagtgccga
     8521 acgatacgca tcaaaatgta gctatttcgc ggcgaaaaag gagcgcgcaa tgaagttctg
     8581 gcgacccggt attaccggca aactgtttct ggcgattttc gccacctgca ttgtcttgct
     8641 gatcagtatg cactgggcgg tgcgtatcag ttttgagcgt ggctttattg attacatcaa
     8701 acacggcaat gaacagcgat tacagctgtt aagtgatgcg cttggcgagc agtatgcgca
     8761 gcatggtaac tggcgcttcc tgcgcaacaa cgatcgcttt gtctttcaga tcctgcgttc
     8821 atttgaacac gataattcgg aagataaacc cggcccgggt atgccgccgc acggctggcg
     8881 tacccagttc tgggtggtcg atcaaaacaa caaagtactg gttggtccgc gagcgccgat
     8941 tccacctgac ggtacacggc gacccattct ggtcaacggt gcggaagtgg gcgcggtgat
     9001 tgcctcccct gttgagcggc tgacccgcaa tactgatatc aatttcgata aacaacagcg
     9061 gcaaaccagc tggctgatcg tcgccctggc aacgttactc gcggcgctcg ccacctttct
     9121 gctggcgcgc ggtttgctgg caccggtaaa acgacttatc gatggcacgc acaaactggc
     9181 ggcgggcgat ttcactaccc gcgtaacgcc caccagtgaa gatgaactgg gcaaactggc
     9241 gcaagacttc aaccagctcg ccagcacgct ggagaaaaac cagcaaatgc ggcgcgattt
     9301 tatggccgat atttctcacg aactgcgtac gccattagcg gtgctgcgcg gtgaactgga
     9361 agccattcag gatggcgtgc gcaaattcac gccggagacg gtggcgtctt tacaggcgga
     9421 ggtcggtaca ctgaccaaac tggttgacga tcttcatcag ttgtcgatgt ctgatgaagg
     9481 cgctctcgcc tatcaaaaag caccggtaga tttgatccca ctgctggaag tggcgggcgg
     9541 cgcatttcgc gaacgattcg ccagccgtgg cctgaaactg caattttccc tgccagacag
     9601 tatcaccgta tttggcgatc gcgaccgttt aatgcagtta ttcaataact tactggaaaa
     9661 cagcctgcgc tacactgaca gcggcggcag cctgaaaatc tctgccgagc agcacgacaa
     9721 aacggtgcgc ctgacctttg ccgacagtgc gccaggtgtc agtgacgatc agctacaaaa
     9781 attgtttgaa cgtttttatc gcaccgaagg ttcccgcaac cgcgccagcg gcggttccgg
     9841 gctggggctg gcgatttgcc tgaacattgt tgaagcccat aatggccgca ttattgccgc
     9901 ccattcgcct tttggcgggg taagcattac agtagagtta ccgctggaac gggatttaca
     9961 gagagaagta tgaccgagtt accaatcgac gaaaacacac cgcgtatttt gatcgtggaa
    10021 gatgaaccga agctggggca gttgctcatt gattatctgc gtgctgcgag ctatgcgccg
    10081 acgcttatca gccacggcga tcaggtactg gcgtatgtgc gccagacacc accggatctg
    10141 atcctgttgg atctgatgct ccctggcacc gatggcctga cattatgccg ggaaattcgt
    10201 cgtttttccg atgttccgat cgtgatggtg acggcaaaaa tcgaagagat cgaccgcctg
    10261 ctggggctgg agattggcgc cgatgactac atctgcaagc cgtacagccc acgggaagtg
    10321 gtagcgcgtg tcaaaaccat tttgcgccgt tgtaaaccgc aacgcgaact tcagcaacag
    10381 gatgccgaaa gcccgttgat tatcgacgaa ggtcgttttc aggcttcatg gcgcggtaaa
    10441 atgcttgacc tgacgcctgc ggaatttcgt ctgcttaaaa cgctctctca cgaaccagga
    10501 aaagtgttct cccgcgagca attgctcaat catctttatg acgactaccg cgtagtaacc
    10561 gaccgcacca ttgacagcca cattaaaaat ctgcgccgca agctggaatc tctcgacgcc
    10621 gaacagtcat ttatccgcgc cgtttacggc gttggttacc gctgggaagc cgacgcctgc
    10681 cgcatcgttt agttttagcg acattacttt gttagccgga gatggcgttc agacccgatc
    10741 cggcaacatt attccacgca tggtcagcaa actgacatta tctctccgcc ctggcaggca
    10801 tcagaaaatc gctcatactt taatcagtaa acagcacctt tagatgctgt tttcgataag
    10861 caatttcaat caaggagtca ttatggctgg ttggtttgaa ctcagtaaga gcagtgataa
    10921 tcagttccgg tttgtgctaa aagcgggcaa tggtgagact atcctcacca gcgagctgta
    10981 tacctcaaaa gcctctgcgg aaaagggcat cgcgtcggtg cgtagcaaca gcccgcaaga
    11041 agagcgctat gagaaaaaaa cggcaagcaa cggcaaattc tatttcaatc tgaaagccgc
    11101 taatcatcaa attatcggct ccagccagat gtacgccacc gcgcaatctc gtgaaaccgg
    11161 aattgcctcc gttaaagcca atggcacaag ccagacggtg aaagacaata cgtaattacg
    11221 atgccgggcg tgggttgttg gcgtccggcg ctttaccaga tcccccctac agcgctacaa
    11281 tgcccgccct taaagtgggg gcctccccta accgcttcat caggtgaagc ggatctgacc
    11341 tgtcatcaga acgagagaat tatgtttaaa ccggaactcc tttccccggc gggaacgctg
    11401 aaaaatatgc gttacgcttt cgcttatggc gcagatgctg tttatgcggg ccagccgcgt
    11461 tactccctac gtgtgcgcaa caacgaattc aaccacgaaa atcttcagct cggcatcaat
    11521 gaagcccacg cgctggggaa aaagttttat gtcgtggtca acattgcacc acacaacgcc
    11581 aagctgaaaa cctttatccg tgacctgaaa ccggtggtgg aaatggggcc ggatgcgctg
    11641 attatgtccg atccagggct gattatgctg gtgcgtgagc acttcccaga aatgccgatc
    11701 cacctctcgg tgcaggctaa cgccgtgaac tgggcgacgg tgaaattctg gcagcaaatg
    11761 ggactgaccc gcgtgatcct ctctcgcgag ctgtcgctgg aagagattga agagatccat
    11821 aatcaggtgc cggatatgga gatcgagatc ttcgttcacg gcgcactgtg catggcctac
    11881 tccggtcgct gcctgctctc tggctatatc aacaagcgcg acccgaacca gggcacctgc
    11941 accaacgcct gccgctggga gtacaacgtc caggaaggga aagaagatga cgttggcaac
    12001 atcgtacaca agtacgagcc gattccggtg caaaatgttg agccgacgct gggtatcggc
    12061 gcgccaaccg acaaagtgtt tatgatcgaa gaagcccagc gtccgggcga gtatatgacc
    12121 gcgtttgaag atgagcacgg cacttacatc atgaactcga aagatctgcg cgccatcgcc
    12181 catgtagaac gcctgaccaa aatgggcgtg cattcgctga aaatcgaagg tcgtaccaaa
    12241 tctttctact attgcgcacg caccgcacag gtttaccgca aagctatcga tgacgccgct
    12301 gcgggaaaac cgttcgatac cagcctgctg gaaactctgg aaggtctggc gcatcgtggc
    12361 tataccgaag gtttcctgcg tcgtcatact cacgacgatt atcagaacta cgaatacggt
    12421 tattcagttt ctgaccgcca gcagtttgtt ggtgagttta ccggtgagcg caagggggac
    12481 ctcgcagcgg tagcggtgaa aaataaattc tccgttggcg acagtcttga gctgatgacg
    12541 ccgcaaggca acattaactt tacccttgag cacatggaaa acgccaaagg cgaagctatg
    12601 ccggtcgcac caggcgatgg ttatactgtg tggctcccgg tcccgcagga tcttgagcta
    12661 aattacgcgc tgctaatgcg taatttctcc ggggaaacca cgcgtaaccc ccacggtaag
    12721 tgattaattt cgattatttt tcccggatgg aaaattctta gaaaccgatc acatacagct
    12781 gcatttatta aggttatcat ccgtttcgct gaaaaacata atccataaaa tgctagctgt
    12841 accaggaacc acctccttag cctgtgtaat ctcccttaca cgggcttatt ttttgtacat
    12901 aagctgtaaa gaaggattga tccctgataa ccgtcttcca ttgaccacat cgatagaatc
    12961 ccccttcata gcacaatgcc tttcacgtaa cggtatcgtg ctcgcccagg ttccggctac
    13021 gcacagccag aacgcgcatg tttgacgctt accaaaaaat attctcactc tccacatttg
    13081 aatgtcagac gagcgacgcc atgtaatcct gcaccttctg tcttcaggtc aactatctgc
    13141 atttttttgc cctgagtaac acagaaatgg gctgcatcat tttttactat attttctgca
    13201 ccagatattc tgcccctggc taaagaagct tcggcttcgg tgtagtattg gttatcgagt
    13261 ttacgctgaa tattactttt atatgcaaga ccaaatttac cgatacttgt ttcatcatta
    13321 tgcacagcac aaccagatat aagaaaaata ctaattaatg atatagcagc tatctttttc
    13381 atctcacctt cccccattaa ataccaacga cactctctag tgtttaaata taataatggc
    13441 atgattatta taattgaata ggattataat aaatgttctg tacaacattt cctacataag
    13501 taggaattac ggacattgag gcccttcagg gtaactccat gggggcttta atatattata
    13561 ttgaagatgc cactgtttag ttgaatatta ggtatatgct cttttttgaa atttataggt
    13621 ggcagccgtt agtattcgct gtccccattg caagctcctg gtggtaacca ctgaatcctc
    13681 catacttgaa ctgacttttt atcctccgac tttcatcctg ttctgactcc accttttgtt
    13741 ttctgctcta cactatctac agaccaatca taaaggcaca tacgatcatg gcagaatttc
    13801 ccgccagctt actgattctt aatggcaaaa gtactgacaa tctacccttg cgcgaagcaa
    13861 ttatgctgtt gcgtgaggaa ggaatgacga tccatgtgcg ggtcacctgg gagaaaggtg
    13921 atgccgcacg atttgtagag gaggcccgga agttgggcgt cgcaacggtg attgccggtg
    13981 gtggcgatgg caccattaat gaagtttcta cggcgttgat tcagtgtgag ggggatgaca
    14041 tacccgcgct gggaattttg ccattaggaa ccgccaatga ttttgccacc agtgtaggga
    14101 ttcctgaggc actggataag gcgctgaaac tggcaattgc cggtaatgcc attgcgatag
    14161 atatggcgca ggtcaacaaa caaacctgtt ttattaatat ggcgacaggc ggatttggga
    14221 cgcgtattac cacagaaacg ccggaaaaat taaaagccgc gctgggtggc gtctcttaca
    14281 tcattcatgg cttaatgcgc atggatactc tgcaaccgga ccgttgtgaa atccgcggtg
    14341 aaaactttca ctggcaaggt gacgccctgg tcattggtat tggtaacggg cgtcaggccg
    14401 gtggcggtca acaattgtgc ccgaacgcgt taattaacga tggcttgctg caactgcgca
    14461 tttttaccgg cgatgaaatt cttccggctc tcgtatcaac cttaaaatct gacgaagata
    14521 acccgaatat tatcgaaggc gcttcgtcgt ggtttgatat acaagcccca cacgaaatca
    14581 cttttaatct tgatggcgaa ccgttgagtg ggcaaaactt ccatattgaa atacttccgg
    14641 cggcgttgcg ttgtcgatta ccaccagatt gtccattatt gcgttaatca aactattctc
    14701 tcttgaacca gggagaatcc gtaatgccgg atgcggtgta aacaccttat ccggcataca
    14761 gaacaatact acaccgtaat taattttacc cgtgtcttct gcaatgccaa tttatccacc
    14821 tctgacacag catcatcggt aacaataatg tcaatttttt cgattggcag tacctgatta
    14881 aaaccgcgac gattaaattt cgatgaatcg agaacggcaa tcactttatt tgcggcggtg
    14941 accatcgccc cgctaatcga ataaccttca ttaaaggtcg taataccatt aaccgcatca
    15001 ataccatcag caccgacaaa cattaaatcg gcattaatat cctgcaatga acgctcggca
    15061 atggaaccat gcatcgagcg cgttttatgg cggaccgtgc caccacagac gaccagagta
    15121 atgtctttat tttcggaaag ggcaaacgct gccgggagac tgttggtaat caccgtgata
    15181 tttttagcgg tcattaatcc ttcggcgata agcattgtgg tacttccgct gtcgagaata
    15241 acggtcatcc cttcatggat cattttgact gccgcctgcg ctattctgtt ttttggcgcg
    15301 ctggcgagct gaaatcgctc tttaaacccg acttcctggg tctcggtttc actattaccg
    15361 gacattattt tcgccgcacc gccatgaaag cgcgtaacca cgcctttttg ttcgagaaag
    15421 cgcaaatcgg cacggattgt cgcttccgag gcagcaaata ctcccgccag atcctgaaca
    15481 agcacggttc cctgttcatt cactaattgg atgatcttat tccttcgctc gaatgaattc
    15541 atcgtcattt acccatattc aattgtggct agtgtaaacg aagggggacc attggtgaat
    15601 gatctgtttt gcacaatacc gggccagcat cacgctggcc cgcggtttca tggaatgagc
    15661 aacactttgc ccggcatggc attacgagcg atgtcacgca ccgcctgggt gaagctttca
    15721 aagcttccac ggtgagcgat taatggatcc aggcttaact tacgttctgt cagcaaccgg
    15781 ctcgccgttt cccactcctg ccccggccaa gggctggaat agttcatcca actgccgata
    15841 accgtcagct ctttacgcaa tattttgcca aacgttgccg atgttaaatg cagatcctga
    15901 tgcaacgtgc ccaccagcgc cagttgggca tgaggaccgg caatctctac cgccagttcg
    15961 acagtttgcg gtacgccagc cgtctcgagg ataagctgat taaagcgcag ttcgcgtaaa
    16021 acgctctgca tttgcggcgc gctcatttcg ctactgttaa atgtttgcat cgcaccgaaa
    16081 gattttgcca gtgccagttt ttctgagcta atgtcgatcg ccgtcacact ctttgctccc
    16141 agcgcgacag cgcactgaat agccagcagg ccaatggttc cggcaccaat aataataacg
    16201 tttttattct cacaaccttg cgctaaatga aaagcatgca ggccaacggt aatcggctca
    16261 ataaaagccc catcctcaat aggcatatcc gtgggtagag caaagacatt ttttcgctta
    16321 acgacaatat attcagcaaa tccaccatca cgccgcgagc caataaaatc atattttgcg
    16381 cactgggaat aaaacccttt caaacactct ggacaagtaa aacagggtaa taacggcaca
    16441 caggcaaccg catcgccagg gtgtaaatca tcaacaccgg atcccaccgc atcaatatag
    16501 ccgctaaatt catggcctaa cgttattgga taataatgtg caccattttt aaatatcctg
    16561 ggtaaatcgg aaccacataa gcccgagctg gcaattttta cccgcacctc atcctgatgt
    16621 ttaatttcag gaatgacgct ttttgcaacg cgcacgatac catcagtatc attcactact
    16681 gatttcataa aaactcctga ttgttaaggg ggataacccc cccctcaggt aaaaattatt
    16741 ctgcgagaac ggctttctct tgtttagtaa aaccacgcgc tctacgccag gtcatgaaaa
    16801 taccggtcag ata
//
LOCUS       DADMGC010000085        16755 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.085, whole genome shotgun sequence.
ACCESSION   DADMGC010000085 DADMGC010000000
VERSION     DADMGC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.085"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="JNZ73_002721"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="JNZ73_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY8732951.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="JNZ73_002722"
     CDS             2219..2767
                     /locus_tag="JNZ73_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HAY8732952.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="JNZ73_002723"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="JNZ73_002723"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HAY8732953.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QSLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /locus_tag="JNZ73_002724"
     CDS             complement(3664..4854)
                     /locus_tag="JNZ73_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HAY8732954.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFSAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JNZ73_002725"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JNZ73_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HAY8732955.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="JNZ73_002726"
     CDS             complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="JNZ73_002726"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAY8732956.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="JNZ73_002727"
     CDS             complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="JNZ73_002727"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAY8732957.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9767..12382
                     /gene="pepN"
                     /locus_tag="JNZ73_002728"
     CDS             9767..12382
                     /gene="pepN"
                     /locus_tag="JNZ73_002728"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAY8732958.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12589..13356)
                     /gene="ssuB"
                     /locus_tag="JNZ73_002729"
     CDS             complement(12589..13356)
                     /gene="ssuB"
                     /locus_tag="JNZ73_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY8732959.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13353..14000)
                     /locus_tag="JNZ73_002730"
     CDS             complement(13353..14000)
                     /locus_tag="JNZ73_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY8732960.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPER
                     VVTAFWTLSASGELWQHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNI
                     DRGLVEMARSYGLSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGI
                     GYLAMNAREFLQTDVVVVAIILYALLGKLADVSAQLLERLWLSWNPAYHLKEATI"
     gene            complement(14012..15157)
                     /gene="ssuD"
                     /locus_tag="JNZ73_002731"
     CDS             complement(14012..15157)
                     /gene="ssuD"
                     /locus_tag="JNZ73_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HAY8732961.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSEHYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(15154..16113)
                     /locus_tag="JNZ73_002732"
     CDS             complement(15154..16113)
                     /locus_tag="JNZ73_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8732962.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPAGARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTIGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16106..16681)
                     /gene="ssuE"
                     /locus_tag="JNZ73_002733"
     CDS             complement(16106..16681)
                     /gene="ssuE"
                     /locus_tag="JNZ73_002733"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAY8732963.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQPH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4184 a         4321 c         4291 g         3959 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agtcgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg agaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcag ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaattatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt tttatgacac ctgccactgc cgtcaataag
     6181 ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta tgtgtctgca
     6241 ggcatctttc cattcaaact aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt
     6301 tagtttcaaa aagaaaaaat atgtaaccaa aagtaaaaat taaggagctt tgtgaacacc
     6361 gtcatatttc catagaaacg tgatgatatt tacagcaatt tttatctatt tatatgattt
     6421 acttatattt aaattaacta aacggaaatt ttgtttctga tggaaacttt atcgacctgg
     6481 cacaaaatcc tctttcagac atccagaatg ccaaaaaata gtatgaaaat tgtgctatca
     6541 gctaagaaaa aactaatccg caataagaat gcgggttatt ttttgtgcgt aaatgttgca
     6601 atgtaatatt ccgtaacagg atgatcgtta tggcgggggt gcaaatagat tgcttgactt
     6661 aacaaacaga taacttgaca gaaaggataa aaaagagcca gcaggcgctg gctctttgag
     6721 gaagaaaatc agaagctggc gttgcgcgga gtacgtggga acggaatcac atcacgcacg
     6781 ttttgcacgc cagttacgta agcaatcagg cgttcaaaac caagaccgaa acctgaatgc
     6841 ggaacagtac cgtagcgacg cagatcgcga taccaccagt aatcttcttt attcaggccc
     6901 atttccagca tacgctcgtc cagaacgtcc agacgttctt cacgctggga gccaccaatg
     6961 atctcaccga tgcccggagc cagaacgtcc atagccgcaa cggttttacc gtcttcgttg
     7021 aggcgcatat agaacgcttt aatatctttc ggatagtttt taaccactac cggtgcttta
     7081 aagtgttctt ccgccagata acgctcatgc tcagaagaga gatcgacgcc ccagtaaacc
     7141 gggttttcaa acttcctgcc gcagttttcg agaatggtca ctgcgtcggt gtaatccacc
     7201 tgtgcgaaat cggcttcaat gaagcgttcc agacgtgaaa cggcatcttt atctacgcgt
     7261 tcagcgaaga atttcatgtc gtctgcgcgt tcttcgagaa ccgctttgaa gacatatttc
     7321 agcatggctt cagccagacc cgcgatatcg ttcaggttag caaacgccac ttccggctcc
     7381 agcatccaga attccgccag gtgacggctg gtgttggagt tttcagcacg gaaagtcggg
     7441 ccgaaggtat aaattttgga taatgcgcaa gcgtaggttt cgccgttcaa ctggccagat
     7501 acggtcagga aagactcttt accaaagaag tctttgtcga aatccacttt gccctgatcg
     7561 ttacgcggca ggttttccag atccagcgta gaaacgcgga acatttcgcc tgcaccttcg
     7621 gtatcagatg cggtaatcag tggcgttgaa acccagaaga atccctgctc gttaaagaag
     7681 cgatgcagcg cctgcgccag cgtatggcga acgcgcgcga cggcaccaat caggtttgtg
     7741 cgcggacgca ggtgagcgac ttcacgcaga tactcaatgc tgtggcgttt tgccgccatc
     7801 gggtaagtgt ctggatcttc aacccaacca gcaacttcaa ccttgctggc ctgaatttca
     7861 aattgttgcc cctggcccgg cgatgccacg actttacccg tcacaatgac cgagcagccg
     7921 gtggtcagac gcaggacgtc ttcattgtaa ttgggcagag aattattgat gacagcctgt
     7981 acaggatcaa agcaggaacc gtcataaacg gcgaggaagg agatgccagc ttttgaatct
     8041 cggcgggtac gtacccatcc gcgtacggtg acttcgctgt caacggctac gcggccctgg
     8101 agtacgtcgg ctacaggcac aacgctcata atattctctc tgttaatagt cggaaaaaat
     8161 aaacacttgt ccacccgtaa atgggggggg atacctatgt tacctggcat ctgcaatcag
     8221 acaagcagaa ttcgcaaatg cagcgaaaga tttcggaatt aaaggtaaaa aagggagccg
     8281 attagctccc tgatgatatt aactggcttt tttaatatgc ggaaggtcga acgctttgcg
     8341 cagcgcccga acaaacgctt tatcatggca gatagttttg ccagggctgt cagaaagttt
     8401 cgccaccggt ttaccgttac actctaccaa cttaatcaca atattcaggg gttttacctg
     8461 ggggatatcg caggtcaggc gagtcccaat accaaaactt aattgcacgc gggaagagaa
     8521 gtggcggtat agctcaaccg ctttgcgtaa atccagattg tcagagaaaa ccagcgtttt
     8581 actctgtgga tcaattccca gcttttcata atgtgcaatg gctttttcac cccattcaac
     8641 cgggtcgcca gagtcatgac gcaggccctg ataccgacta gcgaactcga caccgaaatc
     8701 acgcaggaaa gcatccatag tgatgcagtc ggttaatgca atgccaagtt ggtcgggata
     8761 ctcttccagc caggcagcaa gtgcagctcg ctggctgttg gctagatccg ggctgatttg
     8821 ctgatgtgcc tggaaccatt cgtgtgcctg tgttcccatc ggcgtgaggg aaagccgacg
     8881 cgccagatcg tagttgctgg tgcccacgaa ccaggattcc tgttgcagac gcttaacgat
     8941 ggtttcttgt acttcgcgag aaaaacggcg acgggtgcca aaatccatca gatggaagcg
     9001 cgacatatca agaccggcgg ttaacgccga gaagtcgact aatttgcttt ccagcgtgtc
     9061 gagggcttgc gcaacgtcgg cctgcggtga gcgatagcga tgtaccattt cactgataac
     9121 cgccagcaaa ggaacttccc agaggatgac ttcacgccac ggaccgctta agcgaatatc
     9181 cagcttgcca ttatcgttgg acacggtgac ttgttccggg ttaaagcgga actcgcgtaa
     9241 ccagttaaga tagtcggcct taaagaaagg cagggcagag agccactgat attcatcatc
     9301 ctgcaggcgc aggtgctgca tcgcctgaat ctgttcacga atagcatcgg cataaatacc
     9361 cagcagatcg tcacctcggc aacgaaactc cgccgcgaca tgcacatcgt aatagtgatg
     9421 aaacacggct tgctgcatat gcaacttata agcatctgta tccagcaacg agtgcagaac
     9481 aggagaagcg aattgtgtca taggtgcgca gtagcgtcct cgtacaggag cgtttagtac
     9541 aataaacatc ttcaggaact gctggagtat accttgtttc gcaatttatt gaaccccgat
     9601 cacaccatat gccacctttc tggtcgatgg cattacgcgg tgtatgttat aaaaatgtag
     9661 caataaaggc gtttgtacct gaaaagatga agattctgca tagcgcgatt tacgcaacag
     9721 gaatagactg aacaccagac tctataaaag atgctaaagg ttatttatga ctcaacagcc
     9781 acaagccaaa taccgtcacg attatcgtgc gccggattac cagattactg atattgactt
     9841 gacctttgac ctcgacgcgc aaaagacggt cgttaccgcg gtcagccagg ctgtccgtca
     9901 tggtgcatca gatgctccgc ttcgtctcaa cggcgaagac ctcaaactgg tttctgttca
     9961 tattaatgat gagccgtgga ccgcctggaa agaagaagaa gagggcgcac tggtcatcag
    10021 taatttgccg gagcgtttta cgcttaagat cattaatgaa ataagcccgg cggcgaatac
    10081 ggcgctggaa gggctttatc agtcaggcga tgcgctttgc acccagtgtg aagccgaagg
    10141 tttccgccat attacgtatt atctcgaccg cccggacgtg ctggcgcgtt ttaccaccaa
    10201 aattattgcc gataaaatca aatatccctt cctgctttcc aatggtaacc gcgttgcgca
    10261 aggcgaactc gaaaacggac gccattgggt acagtggcag gacccgttcc cgaaaccgtg
    10321 ctacctgttt gcgctggtgg caggcgactt tgatgtactg cgcgatacct ttaccacgcg
    10381 ttctggtcgc gaagtagcac tggagctgta cgtcgatcgc ggcaaccttg atcgcgcgcc
    10441 gtgggcgatg acctcgctga aaaactccat gaaatgggat gaagaacgct ttggcctgga
    10501 gtatgacctc gacatctata tgatcgtcgc ggtggatttc ttcaatatgg gcgcaatgga
    10561 gaataagggt ctgaatatct ttaactccaa atatgtgctg gcccgcaccg acaccgccac
    10621 cgacaaagat tacctcgata ttgaacgcgt tatcggccat gaatatttcc ataactggac
    10681 cggtaaccga gtgacctgcc gcgactggtt ccagctcagc ctgaaagaag gtttaaccgt
    10741 cttccgcgat caggaattca gctctgacct tggttcccgc gcagttaacc gcatcaataa
    10801 tgtgcgcacc atgcgcggat tgcagtttgc ggaagacgcc agcccgatgg cgcacccaat
    10861 ccgcccggac atggtcattg agatgaacaa cttctacacc ctgaccgttt acgagaaggg
    10921 cgcggaagtg atccgtatga tccacaccct gctgggcgaa gaaaacttcc agaaagggat
    10981 gcaactctat ttcgagcgtc atgatggtag cgcagcgacc tgtgacgact ttgtgcaggc
    11041 gatggaagat gcgtcgaatg ttgatctctc ccatttccgc cgttggtaca gccagtccgg
    11101 tacgccggtt gtgaccgtca aagacgacta caatccggaa actgagcagt acaccctgac
    11161 catcagccag cgcacgccag ccacgccgga tcaggcagaa aaacagccgc tgcatattcc
    11221 gtttgccatc gaattgtatg acaacgaagg caaagtgatc ccgttgcaga aaggcggtca
    11281 tccggtgaat tccgtgctga acgtcaccca ggcggaacag acctttgttt ttgataatgt
    11341 ctacttccag ccggtgcctg cgctgctgtg cgaattctct gcgccagtga aactggaata
    11401 taagtggagc gatcagcaac tgaccttcct gatgcgtcat gcgcgtaatg atttctcccg
    11461 ttgggatgcg gcgcaaagtt tgctggcaac ctacatcaag ctgaacgtcg cccgtcatca
    11521 gcaagggcag ccgctgtctc tgccggtaca tgtggctgac gctttccgcg cggtgctgct
    11581 cgatgagaag attgatccgg cgctggcggc agaaatcctg acgctgcctt ctgtcaatga
    11641 aatggctgaa ctgttcgata tcatagaccc gattgctatt gccgaagtac gcgaagcact
    11701 cactcgtact ctggcgactg aactggcgga tgagctgctg gctatttaca acgcgaatta
    11761 ccagagcgag taccgtgttg agcatgaaga tattgcaaaa cgcactctgc gtaatgcctg
    11821 cctgcgcttc cttgcttttg gtgaaacgca tctggctgac gtgctggtga gcaagcaata
    11881 ccatgaagca aacaatatga ccgatgcgct ggcagcgctt tctgcggcgg ttgctgcaca
    11941 gctgccttgc cgtgacgcgc tgatgcagga gtacgacgat aagtggcatc aggacggtct
    12001 ggtgatggat aaatggttta tcctgcaagc caccagcccg gcggcgaatg tgctggagac
    12061 ggtgcgtggt ctgttgcagc atcgctcatt taccatgagc aacccgaacc gtattcgttc
    12121 gttgattggt gcgtttgcgg gcagcaaccc ggcagcgttc catgccgaag atggcagcgg
    12181 ttaccagttc ctggtggaaa tgcttaccga cctcaacagc cgtaacccgc aggtagcttc
    12241 acgtctgatt gaaccgctga ttcgcctgaa acgttatgat gccaaacgtc aggagaaaat
    12301 gcgcgcggcg ctggaacagt tgaaagggct ggaaaatctc tctggcgatc tttacgagaa
    12361 gataactaaa gcactggctt gatacttttg agccaaaatg cctgatgcga cgcttgacgc
    12421 gtcttatcag gcctacaact ccgttgaaat tgctggtttt gtaggctgga taaggcgttc
    12481 acgccgcatc cggcagccgt gcccaactgc ctgatgcgat gcttgacgtg tcttatcagg
    12541 cctacaagtc cgttgaaaat gctggttttg tgcgccacat ccggcatatt aaccatgacg
    12601 ccgaatcagt tgttcagaat gcccacgccg cattacccgt tgcaatactt ccgcttccag
    12661 ctccgctaat cgcaccgacc ctaatcgacg cggacggggg atatccactg tcaaatccag
    12721 actaattttt ccctcttcaa ttaacaacac ccggtcagcc attgctaccg cttcgctcac
    12781 atcatgcgtc accaacagta cggtaaagcc gtgctgctgc caaagtgaca caatcaaatc
    12841 ctgcatctcg agtcgcgtta aggcgtccag tgccccgagc ggttcatcaa gcaacaataa
    12901 acccggtcga tgaatcaacg ctcttgccag cgccacacgc tgtttctgcc cgccagaaag
    12961 tgcggcaggc cattccccgg cgcgattctc cagtcctacc gcagccaacg cccgacgcgc
    13021 ggcatcgcgc cactgacctt taaggcctaa cccaacgtta tcaatcaccg atttccacgg
    13081 cagcagacgc gcatcctgaa acatcattcg cgtatcatcc tgaatttcag ccagcggtgt
    13141 ggtgcccgct aacacatcgc ctgcggttgg cgtttccaga ccggccagca ggcgcagcag
    13201 ggtactttta ccaccaccgc tgcggcccac caccgccaca aactgacctg ccggaatatg
    13261 taaatccagt tggttcagga cgatattttc cgcgtaatgt ttgcttactg cattgagcaa
    13321 caatggtgtg ccctggttca gacgagcagt attcatatcg tggcctcctt caaatgataa
    13381 gccgggttcc agctcaacca gaggcgctct aacagctgcg cgctgacgtc agcaagtttg
    13441 ccgagcaggg cgtaaagaat aatagcgact accaccacgt ctgtttgcaa aaactctcgc
    13501 gcattcatcg ccagataacc aatgccggaa ttggcagaaa tggtttcggc aacaatcagc
    13561 gtcagccaca tcaggcccaa cgcaaaacgc acgccgacca taattgaggg cagggcatcg
    13621 ggcaggatca catggataaa cagcggtatg ccggataatc catagctacg cgccatctcc
    13681 accagcccgc gatcgatatt acggatgcca tgccaggtgt tgatataaat ggggaacagc
    13741 gtaccgagcg ccaccagaaa gattttcgcg gattcatcaa tgccaaacca caaaatcacc
    13801 agcgggatca gcgccagatg ctgccacagt tcgccgctgg cggagagcgt ccagaacgcc
    13861 gtcaccactc tttccggtga aggcaaaata cgcgtcgaca gccagccaac cgaggaggcc
    13921 agttgccaca ccgccacgat gcccaccggt aaaaaccagg gggcaacgcg caataaccac
    13981 ttcttcactg gcgttgccat catcgtgttc cttagctttg cgcgacttta cgggggataa
    14041 aatcattcgc caccgcttcg ccttgcggat tcagcggctg cggctgggga atttccggaa
    14101 tggcgacatc cagatgtggg aacagcaact cgccaacccg atacgcttct tccagatgcg
    14161 ggtagcccga aagcacaaag ctgtcgatgc caagcgcggc gtattcgttg attcgcgcgg
    14221 cgaccgtagg accatcgccc accagcgccg ttccggcacc gccgcgcact aagccaacgc
    14281 ccgcccataa attggggctg atctccagat tgtcgcgctt gccgttatgt agcgccgcca
    14341 ttcgctgttg tcctacggaa tccgtccggg cgaatgcggc ttgtgctttg gcgatagtct
    14401 catcatcaag atgcgagatt aaccgctcgg cggcctgcca cgcttcgtcg ttagtttcac
    14461 gaacaatcac atgcagacga ataccgaaac gaattttgcg cccatacgcg gcagctttcg
    14521 cccgcacttg ttcgattttc tctttaacaa gttccggcgg ttcgccccag gtgaggtaga
    14581 gatcaacttg ttctgccgcc agctcctggg cgacatctga cgatccgcca aagtaaagcg
    14641 gaggataagg ctgttgaatc ggcgggaaga gcagttttgc tccgcgcaca tgaatatgtt
    14701 taccgttgaa attgacggtt tcccccagca ataagcgccg ccagacctgg gtaaattccg
    14761 ccgaggcttc gtagtgctcg ctatgatcaa ggaacacgcc gtcgcctgcc agctcttgtg
    14821 gatcgctgcc tgtgaccaga ttaaacaacg cacgaccatt tgagagacgg tcaagcgtgg
    14881 cggcctggcg ggcggcaaca gtaggtgagg ttacgctggg acgcagggcg acaagaaact
    14941 tcagccgctg cgtcaccggg atcatcgacg ctgcaaccag ccacgcatct tcacacgatc
    15001 gccccgttgg gatcagcaca ccggtatagc ccagacgatc cgccgcttgc gcaatttgtt
    15061 gcagataacc gtgatcaacc gggcgtgaac cttcttccgt tcccagatag tgcccgtcac
    15121 cgtgggtcgg taaaaaccag aacatattca gactcataat tgttttcctt ccagttgagt
    15181 gggctgccag atgcgctggc gaatatcgac ttttttcggc accagacggt tttcataaaa
    15241 cagatctgcc gtttgctgct gtaaggcggc aacctcggcg ttaaccggtt tgatggtggt
    15301 aggagggcga tgatctaagt aagaggcaat caccggtgcc ggtaagccaa tcgtttttgc
    15361 cagtaaagcg atgctttgtt cgcgctggct gcgggttaac gcatcggcct cactaaaggt
    15421 tgccagtacg ccctgaataa aagcgccgtt tttttctgca tagggacgag ctgccagata
    15481 aaacgatcca gtttgattga gatcggtgcc gtctttcagc acccgcacgc cgccctgtaa
    15541 taacgcagcg gagtagtagg gatcccagat agcccaggcg tcaacgttac cttgctggaa
    15601 cgcggcgcgg gcaccggcag gcgtcaggta agtgggctgg atatcggtga atttaagtcc
    15661 ggcctgacgc agtgcacgca gtaaaaggtt gtgtgaactg gaacctttct gaaaggcaac
    15721 tttgtgacct ttaagatcgg ctacggtttt gatcgggctg ttttctgcca ccagaatcac
    15781 ttcggctttg ggcttcggtg gctcgacgcc cacatatacc aaatcagccc cggcagcctg
    15841 ggcaaagatt ggcggaatat ccccggtact gccgagatca atactgccaa cgtttaacgc
    15901 ttccaacatt tgcggacccg cggggaactc cacccaggag atttttgatt gcggatagcg
    15961 tttttccagt aactggtggc tttttgccag caccatacca atactgcctt tctgatagcc
    16021 tatacgtaac gcttcaggcg aggattctgc agcaaccgca aaagtagaga cgctaagcaa
    16081 tcccgccagc gccagtttaa tgatgttacg catgggcatt acctcgcaga gacagaaggt
    16141 caggaacctg aacatcgcgg cggtgcaatg cctgccagaa agtttctagc gcggtatcaa
    16201 gatggggttg cagatttggc gtgaactggg gtctgtgatg gtaatcaatt aattgtgagt
    16261 catcggcaaa cacgccgtgc aggatctcct gagctttcag tgcgcttaaa actggtttaa
    16321 gggcataatc gaccgccagc agatgggcca cggtaccgcc cgtcgccagc ggtagcacca
    16381 ctttgccttg caaagcgcgc tctggcagca ggtcgagcag ggttttcagc gcgccggaat
    16441 aggcggcttt atacacaggc gtggcgacaa tcagcccatc agcctgttgc agctgttcgg
    16501 tgaaggtctt gagtgccgga ctatcgaaac gagcataaag cagatcttcc ggggcgaagt
    16561 tttgcagatt ccagtgataa acctctacat ctaggccatt tagtttttcc cgcgcatatt
    16621 ccagcaagga gctggagcga gaaggaaagc gaggactacc cgccagggtg atgacacgca
    16681 tactctctcc ttataaccaa ttgttctttt ttttgttaac attgataaca attcggtcag
    16741 tctgtcggag agaca
//
LOCUS       DADMGC010000086        16683 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.086, whole genome shotgun sequence.
ACCESSION   DADMGC010000086 DADMGC010000000
VERSION     DADMGC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.086"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            1..2081
                     /gene="pbpC"
                     /locus_tag="JNZ73_002734"
                     /pseudo
     CDS             1..2081
                     /gene="pbpC"
                     /locus_tag="JNZ73_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708358.1"
                     /note="penicillin-binding protein 1C; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2231..2662
                     /gene="ndk"
                     /locus_tag="JNZ73_002735"
     CDS             2231..2662
                     /gene="ndk"
                     /locus_tag="JNZ73_002735"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAY8732964.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2812..3966
                     /gene="trmG/rlmN"
                     /locus_tag="JNZ73_002736"
     CDS             2812..3966
                     /gene="trmG/rlmN"
                     /locus_tag="JNZ73_002736"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HAY8732965.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4251..5264
                     /gene="rodZ"
                     /locus_tag="JNZ73_002737"
     CDS             4251..5264
                     /gene="rodZ"
                     /locus_tag="JNZ73_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HAY8732966.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5291..6409
                     /gene="ispG"
                     /locus_tag="JNZ73_002738"
                     /gene_synonym="gcpE"
     CDS             5291..6409
                     /gene="ispG"
                     /locus_tag="JNZ73_002738"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAY8732967.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            6520..7794
                     /gene="hisS"
                     /locus_tag="JNZ73_002739"
     CDS             6520..7794
                     /gene="hisS"
                     /locus_tag="JNZ73_002739"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAY8732968.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7812..8432
                     /locus_tag="JNZ73_002740"
     CDS             7812..8432
                     /locus_tag="JNZ73_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HAY8732969.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8443..9621
                     /gene="bamB"
                     /locus_tag="JNZ73_002741"
     CDS             8443..9621
                     /gene="bamB"
                     /locus_tag="JNZ73_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HAY8732970.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTTVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9739..11211
                     /gene="der"
                     /locus_tag="JNZ73_002742"
     CDS             9739..11211
                     /gene="der"
                     /locus_tag="JNZ73_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAY8732971.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11281..11496
                     /locus_tag="JNZ73_002743"
     CDS             11281..11496
                     /locus_tag="JNZ73_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732972.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11493..12863)
                     /gene="xseA"
                     /locus_tag="JNZ73_002744"
     CDS             complement(11493..12863)
                     /gene="xseA"
                     /locus_tag="JNZ73_002744"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAY8732973.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13025..14491
                     /gene="guaB"
                     /locus_tag="JNZ73_002745"
     CDS             13025..14491
                     /gene="guaB"
                     /locus_tag="JNZ73_002745"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAY8732974.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14560..16137
                     /gene="guaA"
                     /locus_tag="JNZ73_002746"
     CDS             14560..16137
                     /gene="guaA"
                     /locus_tag="JNZ73_002746"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAY8732975.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEAQKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16232..>16683)
                     /locus_tag="JNZ73_002747"
     CDS             complement(16232..>16683)
                     /locus_tag="JNZ73_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732976.1"
                     /translation="EAEQQGKNEEQRQHDEWVAERNREIQQEKQRRANAQAAANKRAA
                     TAAANKKARQDKLDAEATADKKRDQSYEDELRSLEIQKQKLALAKEEARVKRENEFID
                     QELKHKAAQTDVVQSEADANRNMTEGGRDLMKSVGKAEENKSDSWFN"
BASE COUNT         3844 a         4170 c         4671 g         3998 t
ORIGIN      
        1 gaagcgctga tcaattatga agatcgctgg ttctggaagc atccgggggt taatccattc
       61 tcggtggcgc gcgcagcatg gcaagatctc acttcgggac gggttatttc cggtggcagc
      121 acgctcacta tgcaggttgc tcgtctgctt gatcctcacc ccaaaacatt tggtggcaaa
      181 attctccagc tctggcgcgc gttgcaactg gagtggcatt tgtctaagcg tgaaattctg
      241 atcttgtatc ttaaccgcgc tccgtttggc gttacgttgc aggggatcgg tgcggcaagt
      301 tgggcttatc tcggaaaatc gcctgcgaat ttaagctatt ccgaggcggc aatgctggcg
      361 gtgttaccgc aagtgcccag ccgactgcgc ccggatcgtt ggccggagcg tgccgaagcc
      421 gcgcgtaata aagtgctcga acggatggct gcgcaaggtg tgtggtcccg tgagcaggta
      481 aaagagtcaa gggaagaacc catctggctg gcaccccgac aaatgccgca actggcaccg
      541 ctgttttcgc gcatgatgct cggtaaaagc aaaagcgaca aaatcgttac tacgctggat
      601 gccggtcttc aacgacgtct ggaagaactg gcgcaaactg gaaagggcgg ttgccaccgc
      661 gcagctcact ggcgatgatc gtggttaatc ataccgatat gcgtgttcgc ggctgggtgg
      721 gatcggttga tctcaacgat gattcacgct ttggtcatgt tgatatggtc aatgcgatcc
      781 gatcgccagg atcggtgctc aaaccgtttg tttatggtct ggcgctggat gaaggcttga
      841 tccacccggc atcactgctg caagacgtcc cccggcgcac cggtgattat cgaccaggta
      901 actttgatag cggttttcat ggcccgatca acatgagcga ggcgctggtg cgctcgctga
      961 acttacctgc tgtgcaggtg ctggaagcct atggaccgaa acggtttgcg gcaaagttac
     1021 gcaatgttgg attgccgtta tatttgccca acggcgctgc gccgaatctt tcactcattc
     1081 tcggcggcgc tggtgcaaaa ctggaagata tggcggcagc gtataccgcg tttgctcgcc
     1141 acggcaaggc aggcaaattg cgcttacagc ctgacgatcc gctgcttgaa cggcctttaa
     1201 tgtcgtcggg ggcggcgtgg atcattcgcc ggattatggc tgatgaagcg caacccttgc
     1261 cggatagtgc cttgccgcgc gtcgccccac tggcatggaa aacgggcacc agctatggct
     1321 atcgtgacgc ctgggcgatt ggggttaacg ctcgctatgt cattgggatc tggactggca
     1381 gacctgacgg cacgcccgtt gttggtcagt ttggctttgc cagtgccgta ccattgttga
     1441 atcaggtcaa taatatctta ctgtcgcgca gtgcgaatct gccggaagac ccgcgcccga
     1501 actcagtcac tcgtggcgtt atctgctggc cgggcggaca gtctttgccg gaaggtgacg
     1561 gtaactgtcg ccgccgcctg gcaacctggc tgctggatgg cagccagccg ccaacgctgt
     1621 tactgtcaga gcaggaaggg attaatggca ttcgcttccc catctggctg gatgacaaag
     1681 ggcagcgcgt cgccgctgac tgcccgcagg cgaagcaaga aatggtaaat gtctggccgc
     1741 taccgctgga accctggctg cccgcatcag aacgtcgcgc tgtgcgctta ccaccggcct
     1801 cgacaatttg cccgccatac ggtcacgatg cccagctccc tctgcagctg acaggcgttc
     1861 gcgatggcgc gattattaaa cgtttaccgg gcgcagcgga agcgactttg ccgttgcaat
     1921 caagtggagg ggcaggtgaa cgctggtggt ttctgaatgg cgaaccgtta actgaacgcg
     1981 ggcgcaacgt gaccctgcat ttgacggata aaggtgatta tcaattgctg gtgatggatg
     2041 atgtggggca aatcgcgaca gtgaaatttg tcatgcaata gtctgttttg tccctgattg
     2101 ttgctaaaac tcattttatt ttaaaaaaaa tgttaccttc atcaatagtc aacggccctg
     2161 ttgctcatta taatccgcgc catctcgtac gctggtacag acaacaacag aacaatttac
     2221 agaggtaaaa atggctattg aacgtacttt ttccatcatc aaaccgaacg cggtagcaaa
     2281 aaacgtcatt ggtaatatct ttgcgcgctt tgaagctgca gggttcaaaa ttgtcggcac
     2341 caaaatgctg cacctgaccg ttgaacaggc acgtggcttt tatgctgaac acgatggaaa
     2401 accgttcttt gatggtctgg ttgaattcat gacctctggc ccgatcgtgg tttccgtgct
     2461 ggaaggtgaa aacgccgttc agcgtcaccg cgatctgctg ggcgcgacca atccggcaaa
     2521 cgcactggct ggtactctgc gcgctgatta cgctgacagc ctgaccgaaa acggtaccca
     2581 cggttctgat tccgtcgaat ctgccgctcg cgaaatcgct tatttcttcg gcgaaggcga
     2641 agtgtgcccg cgcacccgtt aataatttcg taaatgccgc gtgcaaacgt ggcatccgtg
     2701 cgccagaatt tgtacaatgc agcgcccccg gacgagcagc cgctcaccgg ggcgtttctt
     2761 ttttcaaccc tccaggggcc ataacgtgta ataacgaggc cggaatagca tatgtctgaa
     2821 caattagtca cacctgaaaa cgtcaccacg aaagatggaa aaatcaacct gctggatctc
     2881 aaccgtcagc agatgcggga gttttttaaa gatttaggtg aaaaaacctt ccgcgccgat
     2941 caggtgatga agtggatgta tcactattgc tgcgacaact ttgatgagat gaccgacatc
     3001 aacaaagtgt tgcgcggcaa actgaaagag gtggcggaaa tccgcgcacc ggaagtggtt
     3061 gaagaacagc gttcatccga cggcaccatt aaatgggcga tcgccgttgg cgatcagcgc
     3121 gtcgaaacgg tgtatatccc ggaagacgac cgtgccacgc tctgcgtctc ttcgcaggtg
     3181 gggtgtgcgc tggagtgtaa attctgttcc accgcccagc agggctttaa ccgcaacctg
     3241 cgggtatcgg aaattattgg ccaggtgtgg cgtgcggcga aaatcgtcgg cgcagcaaaa
     3301 gtcaccggtc agcgcccaat cactaacgtc gtgatgatgg gcatgggcga gccgctgctc
     3361 aacctgaaca acgtcgttcc ggcgatggaa atcatgcttg atgatttcgg ttttggcctg
     3421 tctaaacgtc gcgtgacgct ttccacttcc ggtgtagttc cggcgctgga taaactgggc
     3481 gatatgatcg acgttgcact ggcaatttcc ctgcacgcgc cgaacgacga aattcgtgac
     3541 gaaattgtgc cgatcaacaa aaagtacaat atcgaaacgt tccttgctgc ggtgcgccgt
     3601 tatctggaga aatccaacgc caatcagggc cgagtcacta ttgaatacgt gatgcttgac
     3661 cacgttaacg acggcactga acacgcgcac caactggcgg aactgctgaa agatacgccg
     3721 tgtaagatca acctgatccc gtggaacccg ttcccgggcg cgccgtatgg acgcagttcg
     3781 aacagccgta tcgaccgttt ctcaaaagtg ctgatgagct acggtttcac cactattgtg
     3841 cgtaaaacgc gtggtgatga tatcgatgct gcctgtggtc agttggcggg cgatgttatc
     3901 gaccgtacga aacgtactct gcgtaaacgg atgcagggtg aagccatcga tattaaagcg
     3961 gtctgatagc cattacgcca cggttacttt atgtaccgtg gcgtaatctt ttatgaatga
     4021 agtattaagt tgcaattgtc tggtcaatat taacggtgcg tttttgctga ctttaaggca
     4081 gtatgtaacg atgcaacagt aagttagcct cagtgaatgt gggctttgtc acgagcacac
     4141 agacggtctt atactgtatg ataacggtta acttaacgga tgtttcgcgg tgtgggtgag
     4201 catgatggtt caccggcatc tcaattctca tttaaacgta cctgcagcga atgaatactg
     4261 aagccacgca cgaccaaaat gaagcactta ctaccggcgc tcgcctgcgt aatgctcgcg
     4321 aacaactagg acttagtcag caggccgttg ccgagcgact ttgcctgaag gtttccacgg
     4381 tacgcgacat tgaagaagat aaggcacccg ccgatcttgc ttcaacattc ctgcgcggat
     4441 atatccgctc ttatgcgcgt ctggtacata ttccagaaga agaactgctg ccagggctgg
     4501 aaaagcaggc tccacttcgg gctgcaaaag ttgcgccaat gcagagtttt tccctcggta
     4561 aacgccgcaa aaaacgcgac ggctggctga tgaccttcac ctggctggtg ttgtttgtgg
     4621 ttatcggcct gagcggtgcc tggtggtggc aagaccacaa agctcagcag gaagagatca
     4681 ccactatggc cgatcaatct tcggcggaac tgagcagtaa tagcgagcag gggcagagtg
     4741 ttccgttaaa tacgtcgaca actacagacc cggctacaac gtccacgccg ccagcgtctg
     4801 tggatactac cgcaaccaac acgcaaacac ctgccgtaac tgcgccagca ccagctgttg
     4861 atccgcaaca gaatgcggtt gtttcgcctt cgcaggcaaa tgttgatacc gccgcgaccc
     4921 cggtaccaac ggcagcaaca acgccagatg gtgctgcgcc gttgccaacc gatcaggctg
     4981 gcgtgaccac gccggcggct gatccgaatg cgctggtgat gaactttact gccgattgct
     5041 ggctggaggt cactgatgct accggtaaaa aattgtttag cgggatgcag cgtaaagacg
     5101 gtaatttgaa tttaaccggc caggcaccgt ataaactgaa aattggtgcg ccagccgcag
     5161 tacagatcca gtatcaaggg aaacctgtcg atctgagtcg ttttatcaga actaaccagg
     5221 ttgcgcgtct gaccctcaat gccgaacaat caccggcgca gtaacagacg ggtaacgcgg
     5281 gagatttttc atgcataacc aggctccaat tcaacgtaga aaatcaacac gtatttacgt
     5341 tgggaatgtg ccgattggcg atggtgctcc catcgccgta cagtccatga ccaatacgcg
     5401 tacgacagat gtcgaagcaa cggtcaatca aatcaaggcg ctggaacgcg ttggcgctga
     5461 tatcgtccgt gtctccgtac cgacgatgga cgcggcagaa gcgttcaaac tcatcaaaca
     5521 gcgggttaac gtgccgctgg tggctgacat ccacttcgac tatcgcattg cgctgaaagt
     5581 agcggaatac ggcgtcgatt gtctgcgtat taaccctggt aatatcggta atgaagagcg
     5641 tattcgcatg gtggttgact gtgcgcgcga taaaaacatt ccgatccgta ttggcgttaa
     5701 cgccggatcg ctggaaaaag atctgcaaga aaagtatggc gaaccgacgc cgcaggcgtt
     5761 gctggaatcc gccatgcgtc atgttgatca tctcgatcgc ctgaacttcg atcagttcaa
     5821 agtcagcgtg aaagcgtctg acgtcttcct cgctgttgag tcttatcgtt tgctggcaaa
     5881 acagatcgat cagccgctgc atctggggat caccgaagcc ggtggtgcac gcagcggggc
     5941 agtaaaatcc gccattggtt taggtctgct gctgtcggaa ggcatcggcg acacgctgcg
     6001 cgtatcgctg gcggccgatc cggtcgaaga gatcaaagtc ggtttcgata ttttgaaatc
     6061 gctgcgtatc cgttcgcgag ggattaactt catcgcctgc ccgacctgtt cgcgtcagga
     6121 atttgatgtt atcggtacgg ttaacgcgct ggagcaacgc ctggaagata tcatcactcc
     6181 gatggacgtt tcgattatcg gctgcgtggt gaatggccca ggtgaggcgc tggtttctac
     6241 actcggcgtc accggcggca acaagaaaag cggcctctat gaagatggcg tgcgcaaaga
     6301 ccgtctggac aacaacgata tgatcgacca gctggaagca cgcattcgtg cgaaagccag
     6361 tcagctggac gaagcgcgtc gaattgacgt tcagcaggtt gaaaaataat aacgtgatgg
     6421 gaagcgcctc gcttcccgtg tatgattgaa cccgcatggc tcccgaaaca ttgagggaag
     6481 cgttgagggt tcatttttat attcagaaag agaataaacg tggcaaaaaa cattcaagcc
     6541 attcgcggca tgaacgatta cctgcctggc gaaacggcca tctggcagcg cattgaaggc
     6601 acactgaaaa acgtgctcgg cagctacggt tacagtgaaa tccgcttgcc gattgtagag
     6661 cagaccccgc tattcaaacg tgcgattggt gaagtcaccg acgtggttga aaaagagatg
     6721 tacacctttg aggatcgcaa tggcgacagc ctgactctgc gccctgaagg gacggcgggc
     6781 tgtgtacgcg ccggcatcga gcatggtctt ctgtacaatc aggaacagcg tctgtggtat
     6841 atcgggccga tgttccgtca cgagcgtccg cagaaagggc gttatcgtca gttccatcag
     6901 ttgggctgcg aagttttcgg tctgcaaggt ccggatatcg acgctgaact gattatgctc
     6961 actgcccgct ggtggcgtgc gctgggtatc ttcgaacacg taactcttga gctgaattct
     7021 atcggttcgc tggaagcacg cgccaattac cgcgatgcgc tggtggcatt ccttgagcag
     7081 cataaagaaa agctggacga agactgcaaa cgccgcatgt acactaaccc gctgcgcgtg
     7141 ctggattcaa aaaatccgga agtgcaggcg cttctcaacg acgctccggc attaggtgat
     7201 tatctggacg aggaatctcg tgagcatttt gccggtctgt gcaaactgct ggagagcgcg
     7261 gggatcgctt acaccgtaaa ccagcgtctg gtgcgtggtc tggattacta taaccgtacc
     7321 gttttcgagt gggtgactaa cagtctcggc tcccagggca ccgtgtgtgc aggcggtcgt
     7381 tatgacggtc ttgtggaaca actgggcggt cgtgcaacac cggctgtcgg ttttgcgatg
     7441 ggcctcgaac gtcttgtatt gttagtacag gccgttaatc cggaatttaa agccgatcct
     7501 gttgtcgata tatacctggt ggcttcaggt gctgatacac aatctgcggc tatggcatta
     7561 gctgagcgtc tgcgtgatga attactgggc gtgaaattga tgaccaacca cggcggcggc
     7621 aactttaaga aacagtttgc ccgtgctgat aaatggggtg cccgcgttgc tgtggtgctg
     7681 ggtgagtctg aagtggctaa cggcacagca gtagtgaagg atttgcgctc tggtgagcaa
     7741 acggcagttg cgcaggatag cgtagccgcg catttgcgca cgttactggg ttaaggaagg
     7801 agaaggacag cgtggaaatt tacgagaacg aaaacgacca ggtagaagcg gttaaacgct
     7861 tttttgctga aaatggcaaa gcactggctg ttggggtgat tttgggcgtt ggcgcactga
     7921 ttggctggcg ctactggaac agccatcagg ttgattctgc acgctccgct tctcttgcct
     7981 atcaaaatgc ggttaccgca gtgagcgaag gcaaaccgga tagcatcccg gcggcggaaa
     8041 aatttgctgc tgaaaataaa aatacttatg gtgcgctggc ttctttggaa cttgcgcagc
     8101 aatttgttga caaaaatgaa ctagagaaag ctgccgccca gttacaacag gggctggcag
     8161 acacgagcga tgaaaatctc aaagccgtga taaatctgcg tcttgctcgc gttcaggtac
     8221 agctcaagca ggctgatgcc gcgctgaaaa cccttgatac catcaaaggt gaagggtggg
     8281 ctgccattgt tgccgacctg cgtggtgaag cattgctgag caaaggtgat aagcaaggtg
     8341 cgcgtagtgc atgggaagca ggcgtgaaaa gcgatgttac tccggcactg agcgaaatga
     8401 tgcagatgaa aattaataat ttgtccatct gagagggacc cgatgcaatt gcgtaaatta
     8461 ctgctgccag gactgctttc cgttaccctt ttaagcggct gttcgctgtt taacagcgaa
     8521 gaagatgtgg taaagatgtc cccattgcca accgttgaaa accagtttac gccgaccacg
     8581 gcgtggagca cttccgttgg tagcggcatt ggcaacttct attccaatct tcatccggca
     8641 ctggcggaca acgttgtcta tgcagcggac cgcgctggtt tggtaaaagc gctgaatgcg
     8701 gatgatggca aagaaatctg gtctgtcagc ctggccgaga aagatggctg gttctctaaa
     8761 gagcctgcat tactttctgg cggtgtgacc gtgtctggtg ggcatgtcta cattggcagc
     8821 gaaaaggcgc aggtttacgc gctgaatacc agcgatggta ctgtggcatg gcaaactaca
     8881 gtcgcgggtg aagcactttc gcgcccggtg gtcagcgacg gtctggtgtt aatccacacc
     8941 agtaacggtc agttacaagc gctgaacgaa gctgacggcg ctgtcaaatg gacagttaac
     9001 ctcgatatgc cttcgctctc tttgcgtggc gagtctgcgc cggcaacggc ttttggtgcg
     9061 gctgtcgtgg gtggcgataa tggtcgcgtc agcgcagtgc tgatggaaca gggccagatg
     9121 atttggcagc agcgtatttc ccaggcgacc ggttctaccg aaattgaccg tctgagcgat
     9181 gttgacacga ctcccgtcgt tgttaacggc gttgttttcg cgctggccta taatggtaac
     9241 ctgacggcgc ttgatctgcg cagtggtcag attatgtgga aacgcgaact gggttcggtg
     9301 aatgatttca tcgtcgatgg caatcgcatc tatctggtcg atcaaaatga ccgggtgatg
     9361 gcgttgacca ttgatggcgg cgttacgctg tgggcacaaa gcgatctgct gcatcgcctg
     9421 ctgacttctc cggtgctgta taatggcaac ctggtggtcg gtgacagtga aggttatctg
     9481 cactggatta acgtcgaaga tggtcgtttc gttgcccagc aaaaagttga tagttccggt
     9541 ttccagactg aaccggttgc cgctgacggc aaactgctga tccaggcaaa agacggaacc
     9601 gtgtactcta ttacacgtta atcgtctctg tcgttcactt tgaaaacggc tcctggacag
     9661 gggccgtttt cctgttttta acaacgacgc gaatatagcg tgcgttgtct gatgatttat
     9721 aaaaatgagg ctttaaacat ggtacctgtg gtcgcgcttg tcgggcgccc taacgtagga
     9781 aaatccacgt tatttaaccg tctaactcgc acccgagatg cgctggttgc ggatttcccg
     9841 ggtctgactc gtgaccgtaa gtacggtcgt gcggaaattg aaggccgtga gtttatctgt
     9901 attgataccg gcgggattga tggcacagaa gacggtgtag aaacccgcat ggcggaacag
     9961 tcgctgctgg cgattgaaga agcggacgtc gtactgttta tggtggatgc gcgcgcgggc
    10021 ctgatgccgg cagatgaagc gattgccaaa catctgcgct cccgtgaaaa accgaccttc
    10081 ctggtggcaa acaaaactga cggtctggat cccgatcagg cagtggttga tttctactcg
    10141 cttggtttag gtgaaatcta cccgatcgcc gcgtctcacg gtcgtggcgt attaagtctg
    10201 ctggagcatg tgctgctgcc gtggatggaa gatctcgcac cgcaagagga agtcgacgaa
    10261 gacgctgaat actgggcgca atttgaagcg gaagagaacg gcgaagaaga agaggaagac
    10321 gacttcgacc cgcaaagtct gccgatcaaa ctggcgattg tgggtcgtcc gaacgtaggt
    10381 aagtctacac tcactaaccg tattcttgga gaagagcgcg ttgttgttta cgacatgcct
    10441 ggcacgacgc gtgacagcat ctacatccca atggaacgcg atggacgtga gtatgtgctc
    10501 attgacaccg ctggcgtacg taaacgcggc aaaatcaccg atgctgtaga gaaattctcc
    10561 gtaatcaaaa cgttgcaggc cattgaagac gccaacgtgg tgatgttagt gattgatgcg
    10621 cgcgaaggta tttccgatca ggatctctcg ctgctgggct ttattctcaa tagtgggcgc
    10681 tcacttgtca ttgtggtgaa taagtgggat ggcctgagcc aggaagtgaa agagcaggtg
    10741 aaagaaacgc tggacttccg tctgggcttt atcgattttg ctcgtgtgca ctttatctct
    10801 gccttgcacg gcagtggtgt tggtaacttg tttgaatcag tacgtgaagc gtatgacagc
    10861 tccacccgtc gtgtggggac ctctatgctg acgcgcatca tgacgatggc tgttgaagat
    10921 caccaaccgc cgctggtacg cggtcgtcgt gtgaagctga aatatgccca cgctggtggt
    10981 tataacccgc cgattgtggt gattcacggt aatcaggtga aagacctgcc tgattcctac
    11041 aagcgctact tgatgaacta cttccgcaaa tcgctggacg taatgggatc gccgattcgt
    11101 attcagttca aagaagggga aaacccgtat gcgaacaagc gtaacaccct gacgccaacc
    11161 cagatgcgta aacgtaagcg tctgatgaag cacatcaaga aaaataaata attatccgta
    11221 agtattttat ctgccattcc ctctatgaat gtagagggaa tggctaccgg aggaattcat
    11281 atggaactgc attgtccaaa atgtcagcat gtgcttgatc aggataacgg ccatgcgcgt
    11341 tgcccatcct gcggggaatt tattgaaatg aaagctcttt gtccagactg ccatcaaccg
    11401 ttgcaggtgt taaaagcctg cggtgcagta gattacttct gtcagcacgg gcatggcttg
    11461 atatcgaaaa aacgcgttga attcgtgctg gcttaatgaa cctttttacg cgattttttt
    11521 accggctgga tgttttttac ttcactttct atccagccgt cttccagacg tgtggttagc
    11581 atttcacccg ctttaacttg cttcactttt ttcagtacat tgccgtcagt agcagtagta
    11641 acgctgtatc cacgcgccag cgttgacagt gggcttacgg cttcgaggtg cgttactgca
    11701 ttaccgaaac gttcacgcgt ggcgctaagc tgtgcgcgca ggatttctgc taaacgatat
    11761 tccagttgct gaatgcgcgt ttgcgtgcga tgaatcttcg gctgtggatt ttgctgattc
    11821 agccgctgtg ttaatcgctg ctgctgttgc ccggcacgct taagctgatt ttccagcgca
    11881 aagctcatcc gtttttgcag gcgttcaagc atggtttgct ggcgtgccag ccggagctgc
    11941 ggatgctgct gctgcaagcg atgatgaatc tgcgtaaagc gacgcgtacg gttggcgaga
    12001 taataatcca tcgccatctc cagccgttga cgggccgatt gcacctggcg cagtaactct
    12061 tgctgattac ggctcactac ttcagcggcg gcagacggcg ttggcgcacg cagatcggca
    12121 acaaaatcgg caatggtcac atccgtctca tgcccgacgg cgctgacgac cggaatgcgg
    12181 ctggcaaaaa tcgcccgcgc tacgcgttcg tcgttaaaac tccataaatc ttccagcgaa
    12241 ccgccgccgc gcccaacgat caacacgtcg cactcattgc gctgattcgc cagttcaatg
    12301 gcgcgaacaa tttgccccgg cgcgtcatcg ccctgaacgg aggtggggta gatgatcacc
    12361 ggtagagaag gatcgcgacg ttttaacaca tgcaaaatat catgtagcgc agcaccggtt
    12421 tttgaggtga tcacaccaac gcaatgcgca ggggagggaa gtggtttttt gtattgctga
    12481 tcgaacaaac cttcagcctg caacttcgct ttgagctgtt cgtacttctg ttgcagcagc
    12541 ccttcaccgg ccggctgcat actctcaacg attatctggt agtcgccgcg cggctcgtag
    12601 agcgtaatat tggcgcgaac taaaacttgt tgcccatgct gcggacggaa ggtgacccgg
    12661 cggttgctgt tgcggaacat cgcgcagcgt acctgggcgg tgtcgtcttt gagtgtaaag
    12721 taccagtgac cggaagccgg ttgtgtgaaa ttagagattt cgccgctgat ccaaacctgt
    12781 cccatctcat gctcaagcag cagacgaacc gtttgattca ggcgactaac ggtaaaaatt
    12841 gcaggggatt gagaaggtaa catgtgagcg agatcaaatt ctaaatcagc aggttattca
    12901 gtcgatagta acccgccctt cggggatagc aagcattttt tgcaaaaagg ggtagatgca
    12961 atcggttacg ctctgtataa tgccgcggca atatttatta accactctgg tcgagatatt
    13021 gcccatgcta cgtatcgcta aagaagctct gacgtttgac gacgttctcc tcgttcctgc
    13081 tcattctacc gttctgccga atactgctga cctcagcacc cagctgacga aaactattcg
    13141 tctgaatatc cctatgcttt ccgcagcaat ggataccgta acggaagcgc gcctggctat
    13201 tgctctggct caggaaggcg gtatcggctt tatccacaaa aacatgtcca ttgaacgcca
    13261 ggcagaagaa gttcgccgtg tgaaaaaaca cgaatctggt gtggtgactg atccgcagac
    13321 tgttctgcca accacgacgc tgcgcgaagt gaaagaactg accgagcgta acggttttgc
    13381 gggctatccg gtcgttaccg aagaaaacga actggtgggt attatcaccg gtcgtgacgt
    13441 gcgttttgtt accgacctga accagccggt tagcgtttac atgacgccga aagagcgtct
    13501 ggtcaccgtg cgtgaaggtg aagcccgtga agtggtgctg gcaaaaatgc acgaaaaacg
    13561 cgttgaaaaa gcgctggtgg ttgatgacga attccacctg atcggcatga tcaccgtgaa
    13621 agacttccag aaagcggaac gtaaaccgaa cgcctgtaaa gacgagcaag gccgtctgcg
    13681 tgttggtgct gcagttggcg caggtgcggg taacgaagag cgtgttgacg cgctggttgc
    13741 cgctggcgtt gacgttctgc tgatcgactc ctcccacggt cactcagaag gcgttttgca
    13801 gcgtatccgt gaaacccgtg ctaaatatcc ggatctgcaa atcatcggcg gcaacgtggc
    13861 aacagctgca ggtgcacgcg ctttggcaga agctggttgc agcgcggtta aagtcggtat
    13921 cggccctggt tctatctgta cgactcgtat cgttactggc gtaggtgttc cgcagatcac
    13981 tgccgttgct gacgcagttg aagccctgga aggcaccggt attccggtta tcgcagatgg
    14041 tggtattcgc ttctccggcg acatcgccaa agctatcgcc gctggcgcaa gcgcggtgat
    14101 ggtaggttcc atgctggcgg gtactgaaga atctccgggt gaaatcgaac tctaccaggg
    14161 ccgttcttat aaatcttacc gtggtatggg ttccctgggc gcgatgtcca aaggttcctc
    14221 tgaccgttat ttccagagcg ataacgctgc cgacaaactg gtgccggaag gtatcgaagg
    14281 tcgcgtagct tataaaggtc gcctgaaaga gatcattcac cagcagatgg gcggcctgcg
    14341 ctcctgtatg ggtctgaccg gctgtggtac tatcgacgaa ctgcgtacta aagcggagtt
    14401 tgtacgtatc agcggtgcgg gcattcagga aagccacgtt cacgacgtga ccattactaa
    14461 agagtccccg aactaccgtc tgggctcctg attctcttcg cccgacttca tgtcgggcga
    14521 tttatattat ctgtttcact tgcctcggaa taagcgtcaa tgacggaaaa cattcataag
    14581 catcgcatcc tcattctgga cttcggttct cagtacactc aactggttgc gcgccgcgtg
    14641 cgtgagctgg gtgtttactg cgaactgtgg gcgtgggatg tgacagaagc acaaattcgt
    14701 gacttcaatc caagcggcat tattctttcc ggcggcccgg aaagtactac tgaagaaaac
    14761 agtccgcgtg cgccgcagta tgtctttgaa gcaggcgtac cgatattcgg cgtttgctat
    14821 ggcatgcaga ccatggcaat gcagttgggc ggtcacgttg aagcctctaa cgaacgtgaa
    14881 tttggctacg cgcaggtaga agtcgtaaac gacagcgcac tggttcgcgg tatcgaagat
    14941 gcgctgaccg cagacggtaa accgctgctc gatgtctgga tgagccacgg cgataaagtt
    15001 accgctatcc cgtccgactt cgtcaccgta gccagcaccg aaagctgccc gtttgccatt
    15061 atggctaacg aagaaaaacg cttctatggc gtacagttcc acccggaagt gactcacacc
    15121 cgccagggta tgcgcatgct ggagcgtttt gtgcgtgata tctgccagtg tgaagccctg
    15181 tggacgccag cgaaaattat cgacgatgct gtagctcgca tccgcgagca ggtaggcgac
    15241 gataaagtca tcctcggcct ctctggtggt gtggattcct ccgtaaccgc aatgctgctg
    15301 caccgtgcta tcggtaaaaa cctgacttgc gtattcgtcg acaacggcct gctgcgtctc
    15361 aacgaagcag agcaggttct ggatatgttt ggcgatcact ttggtcttaa cattgttcac
    15421 gtaccggcag aagatcgctt cctgtcagcg ctggctggcg aaaacgatcc ggaagcaaaa
    15481 cgtaaaatca tcgggcgcgt tttcgttgaa gtattcgatg aagaagcgca gaaactggaa
    15541 gacgtgaagt ggctggcgca gggcaccatc taccctgacg ttatcgaatc tgcggcttct
    15601 gcaaccggta aagcacacgt catcaaatct caccacaacg tgggcggcct gccgaaagag
    15661 atgaagatgg gcctggttga accgctgaaa gagctgttca aagacgaagt gcgtaagatt
    15721 ggtctggagc tgggcctgcc gtacgacatg ctgtaccgtc atccgttccc gggaccaggc
    15781 cttggcgttc gtgttctggg tgaagtgaag aaagagtact gtgacctgct gcgccgtgct
    15841 gacgccatct tcattgaaga actgcgtaaa gcggacctgt acgacaaagt cagccaggcg
    15901 ttcaccgtct tcctgccagt acgttccgtt ggcgtaatgg gcgatggtcg taagtatgac
    15961 tgggttgtct ctctgcgtgc tgtcgaaacc atcgacttta tgaccgcaca ctgggcgcat
    16021 ctgccgtacg atttcctcgg tcgcgtttcc aaccgcatta tcaatgaagt gaacggtatt
    16081 tcccgcgtgg tgtatgacat cagcggcaag ccgccagcta ccattgagtg ggaatgattt
    16141 tgatccctgc actatgaatg aacaaaaccc tccgttacta cagagggttt tttatcttca
    16201 agaatcatag gcttgaagtt actaacatcg attaattaaa ccagctgtcc gatttgttct
    16261 cttctgcttt gcccacgctt ttcatcagat cgcgaccgcc ttcagtcata tttctgtttg
    16321 cgtcagcttc agattgcacc acatcggttt gcgcagcttt gtgcttcagt tcctgatcga
    16381 taaattcgtt ttcgcgctta acgcgggctt cttctttcgc cagcgccagt ttttgtttct
    16441 gaatctctaa gctgcgtagc tcatcttcat aactttgatc gcgttttttg tccgcagtgg
    16501 cttcggcgtc cagtttatcc tgacgagctt tcttatttgc cgctgccgtt gccgctcttt
    16561 tgttcgcggc ggcctgggca tttgcgcgac gttgcttctc ttgctggatt tccctgttgc
    16621 gctccgcgac ccattcgtca tgctgccttt gctcttcatt tttaccttgc tgttccgctt
    16681 ctg
//
LOCUS       DADMGC010000087        16322 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.087, whole genome shotgun sequence.
ACCESSION   DADMGC010000087 DADMGC010000000
VERSION     DADMGC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.087"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(85..2061)
                     /gene="speA"
                     /locus_tag="JNZ73_002748"
     CDS             complement(85..2061)
                     /gene="speA"
                     /locus_tag="JNZ73_002748"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HAY8732977.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYEVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="JNZ73_002749"
     CDS             complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="JNZ73_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HAY8732978.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2337..2552
                     /locus_tag="JNZ73_002750"
     CDS             2337..2552
                     /locus_tag="JNZ73_002750"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8732979.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2856..4010
                     /gene="metK"
                     /locus_tag="JNZ73_002751"
     CDS             2856..4010
                     /gene="metK"
                     /locus_tag="JNZ73_002751"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAY8732980.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4447..5841
                     /locus_tag="JNZ73_002752"
     CDS             4447..5841
                     /locus_tag="JNZ73_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY8732981.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWRTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5918..6415
                     /locus_tag="JNZ73_002753"
     CDS             5918..6415
                     /locus_tag="JNZ73_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HAY8732982.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6510..7217
                     /gene="endA"
                     /locus_tag="JNZ73_002754"
     CDS             6510..7217
                     /gene="endA"
                     /locus_tag="JNZ73_002754"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HAY8732983.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7297..8028
                     /gene="rsmE"
                     /locus_tag="JNZ73_002755"
     CDS             7297..8028
                     /gene="rsmE"
                     /locus_tag="JNZ73_002755"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAY8732984.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8041..8991
                     /gene="gshB"
                     /locus_tag="JNZ73_002756"
     CDS             8041..8991
                     /gene="gshB"
                     /locus_tag="JNZ73_002756"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAY8732985.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9028..9663
                     /locus_tag="JNZ73_002757"
     CDS             9028..9663
                     /locus_tag="JNZ73_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HAY8732986.1"
                     /translation="MTAPFFPRILGAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKITPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTAPADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9663..10079
                     /gene="ruvX"
                     /locus_tag="JNZ73_002758"
     CDS             9663..10079
                     /gene="ruvX"
                     /locus_tag="JNZ73_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAY8732987.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10255..11235)
                     /locus_tag="JNZ73_002759"
     CDS             complement(10255..11235)
                     /locus_tag="JNZ73_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAY8732988.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11253..11957
                     /gene="yggS"
                     /locus_tag="JNZ73_002760"
     CDS             11253..11957
                     /gene="yggS"
                     /locus_tag="JNZ73_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HAY8732989.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11975..12541
                     /gene="yggT"
                     /locus_tag="JNZ73_002761"
     CDS             11975..12541
                     /gene="yggT"
                     /locus_tag="JNZ73_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HAY8732990.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            12538..12828
                     /locus_tag="JNZ73_002762"
     CDS             12538..12828
                     /locus_tag="JNZ73_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HAY8732991.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANNHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12836..13429
                     /locus_tag="JNZ73_002763"
     CDS             12836..13429
                     /locus_tag="JNZ73_002763"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HAY8732992.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITHEPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13422..14558
                     /gene="hemW"
                     /locus_tag="JNZ73_002764"
     CDS             13422..14558
                     /gene="hemW"
                     /locus_tag="JNZ73_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HAY8732993.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRSRLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14872..15835
                     /locus_tag="JNZ73_002765"
                     /pseudo
     CDS             14872..15835
                     /locus_tag="JNZ73_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15880..>16322
                     /locus_tag="JNZ73_002766"
     CDS             15880..>16322
                     /locus_tag="JNZ73_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAY8732994.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYA"
BASE COUNT         4039 a         4209 c         4106 g         3968 t
ORIGIN      
        1 cagctagatt atttttaaga atatataaaa agaaagcggg ccgatttacg taaataaatc
       61 gacccgccag cggaataatc ttaattattc atcttcaaga taagtataac cgtacaaacc
      121 tgcctcgaac tcttcaagga actgttgttg cagttcagca tcaagatcgg ttttcttcac
      181 ttgatcgcgg aactgggtta acagcgtttt cggatcgagc tgtacatatt gcagcatgtc
      241 cgccacggta tcgccttcgt cagacagttc tacttctacg ctaccgtcag ggaacacgaa
      301 cacgtcaact gcttcggtat caccgaacag gttgtgcata ttgccaagga tctcctgata
      361 tgcaccgacc ataaagaaac cgagcatcgg cggattctct ggatcgtact ccggcattgg
      421 cattgtcgtg gcaataccat caccatcaat atagtggtcg atagcaccgt cagagtcaca
      481 ggtaatatcc agcagcacag cgcgacgttc cggcacttga tccagccctt ccagcggtag
      541 aaccgggaac aactggtcga tcccccatgc gtccggcatt gactggaaca gcgagaaatt
      601 gacgtacatt ttgtccgcca tacgttcctg cagttcgtcg attatcggac gatgcgcacg
      661 gttttgcgga tccagttgct tctgcacttc atggcacatg ctcagataga gttgttctgc
      721 ccatgcacgt tcttgcaggc taaaggtgcc ggaagagtag ccgatatgaa tgtcgtgcag
      781 atccatctgg ctgtcgtgta accattcacg caaagaacgg cgagttcccg gttcgtgcat
      841 ttcctgccag gtttcccaca tgctttgcag cgcacgcggc gcatcttctg cgggcgcggt
      901 cggcaccgta tattcgttac gttccacgcc gatgatattc gacaccagca cggtatggtg
      961 cgcagtcacc gcacggcccg attcggtgat taccgtcgga tgcggcagac cattttcttc
     1021 acacgcatcg ccaatcgccc agatgatgtt gttggcgtat tcattgaggc catagtttac
     1081 ggagcagtca gactgcgaac gggtgccttc ataatccacg cccagaccgc cgccgacgtc
     1141 gaagcactga atattgacgc ccagcttgtg cagttctaca tagaaacgcg cggattcacg
     1201 aacgcctgtc gcgatatcgc gaatattcgc catctgcgaa ccgaggtgga agtgcagtag
     1261 ttgcaggctg tcgagacgcc cggcttcacg cagggtttca accagttgca gtacctgagt
     1321 cgcagccagg ccgaacttcg atttttcccc gccggaggac tgccatttac ccgagccctg
     1381 cgaagccaga cgtgcacgca cgcccagacg aggaacgaca ttcagacgtt ctgcttcatc
     1441 cagcacaatg gcgatttctg acatcttctc aatgaccaga tagaccttgt gccccatctt
     1501 ctcgccaatt aatgccaggc ggatatattc gcggtcttta taaccgttgc agacgatgac
     1561 gctacgagtc atgccagcat gtgccagcac tgccatcaac tcggctttgg aaccggcttc
     1621 cagacccagc ggttcacccg aatgaatcag ggactcgatc acgcggcggt gctggttaac
     1681 tttgatcgga taaacaagga agtaatcgcc gttatagccg taggattccc tcgcacgttt
     1741 gaacgcggcg ttaatggaac gcaaacggtg ctgcaggatc tgtgggaaac agaacagtgc
     1801 aggcagacgc tgaccctgtg cttcacgagt tttcactaac tgcgcgagat cgacgcgagc
     1861 ttccgggacg tccgggtccg ggcacacgct gatgtggccc agctcgttaa cctcatagta
     1921 gttattgccc caccaggcaa tattgtaagt acgcagcatc ttgctggctt cctgggagct
     1981 cattgcaacc tcctgcatgg agcgtagtac accgtgttcg cccgctgacg aaggcaaacc
     2041 catagacatg tcgtcagaca tagcgaacct caaattattt tattaagtgt aaaacagtta
     2101 acgactgtcg cagcctggaa ctgcgataac aacccataag cacatggatt ttccagcagt
     2161 gtatgctgac gctccgactg cgcaaccggt ttctttttca taacattatt aagcacataa
     2221 ccgaacgtaa gtgtgacagt tcggcgaaac cacgagaaaa ctcttgtttt tacaagagcg
     2281 cccttgttca gtcctcagta actgtaacca gctcttgaat cctgagaagc gccgagatgg
     2341 gtataacatc ggcaggtatg caaagcagag atgcagagtg cggggaacga atcttcacca
     2401 gaacggtgag acaggttaag cagcagacaa cggttcatta tttcgtatca cctccacggc
     2461 cgcctgttaa gacgaaccca caagccaaaa ctctgatttc aacccggctg gaagtggcaa
     2521 cacgaaaaaa acgtcgtgtg ctttttattt aagccgcgcg ccgcgtttta taccccacaa
     2581 tggcagaaaa ttgcaaaaga taaatacgca gaatgccggc attgtcagga aaaatttcca
     2641 gccacgtttt taacagaatg agacacgatt caaaaaaaag tggaaatcgg gtgaagaatt
     2701 gacctaaaat agccatccag atgttaatcc atccataccg attaacactc agactgccag
     2761 tgtttttaac ctgcagagtc gtggtaggat ccgctaccac agaaaatcca cacaacagtt
     2821 tgagctaacc aaattctctt taggtgatat taaatatggc aaaacacctt tttacgtccg
     2881 agtccgtctc tgaagggcat cctgacaaaa ttgctgacca aatctctgat gccgttttag
     2941 acgcgatcct cgaacaggat ccgaaagcac gcgttgcttg cgaaacctac gtaaaaaccg
     3001 gcatggtttt agttggcggc gaaatcacca ccagcgcctg ggtagacatc gaagagatca
     3061 cccgtaacac cgttcgcgaa attggctatg tgcattccga catgggcttt gacgctaact
     3121 cctgtgcggt tctgagcgct atcggcaaac agtctcctga catcaaccag ggcgttgacc
     3181 gtgccgatcc gctggaacag ggcgcgggtg accagggtct gatgtttggc tacgcaacta
     3241 atgaaaccga cgtgctgatg ccagcaccta tcacctatgc acaccgtctg gtacagcgtc
     3301 aggctgaagt gcgtaaaaac ggcactctgc cgtggctgcg cccggacgcg aaaagccagg
     3361 tgactttcca gtatgacgac ggcaaaatcg ttggtattga tgctgtcgtg ctttccactc
     3421 agcactctga agagatcgac cagaaatcgc tgcaagaagc ggtaatggaa gagatcatca
     3481 agccaattct gcccgctgaa tggctgactt ctgccaccaa attcttcatc aacccgaccg
     3541 gtcgtttcgt tatcggtggc ccaatgggtg actgcggtct gactggtcgt aaaattatcg
     3601 ttgataccta cggcggcatg gcgcgtcacg gtggcggtgc attctctggt aaagatccat
     3661 caaaagtgga ccgttccgca gcctacgcag cacgttatgt cgcgaaaaac atcgttgctg
     3721 ctggcctggc cgatcgttgt gaaattcagg tttcctacgc aatcggcgtg gctgaaccga
     3781 cctccatcat ggtagaaact ttcggtactg agaaagtgcc ttctgaacaa ctgactctgc
     3841 tggtacgtga gttcttcgac ctgcgcccat acggtctgat tcagatgctg gatctgctgc
     3901 acccgatcta caaagaaacc gcagcatacg gtcactttgg tcgtgaacat ttcccgtggg
     3961 aaaaaaccga caaagcgcag ctgctgcgcg atgctgccgg cctgaagtaa tctttcttca
     4021 cctgcgttca aaggccagcc tcgcgctggc ctttttcttt tacctcaccg cggtggatag
     4081 gcgttcacgc cgcatccggc aaaaaaactg cccgcacaat aacatcattc ttcctgatca
     4141 cgtttcacgg caaattacca tcacaattga aaccgattac accaaccaca ccagcaaaat
     4201 atttgtaata ttttcatatt attattcagc tttcacagtt gttacatttc ttttcagtaa
     4261 agtcttaatt gcagatgaca gcgtttaatc tatgatgata taactcaatt attttcatgc
     4321 acttaaatca taactaagat aaatgttagt gtaagcgatt acactgatgt gatttgcttc
     4381 acatcttttt acgtcgtact cacctatctt aattcacaat aaaaaataac catattggag
     4441 ggcatcatgc ctgacgctaa aaaacagggg cggtcaaaca aggcaatgac gtttttcgtc
     4501 tgcttccttg ccgctctggc gggattactc tttggcctgg atatcggtgt aattgctggc
     4561 gcactgccgt ttattgcaga tgaattccag ataacttcgc acacgcaaga atgggtcgta
     4621 agctccatga tgttcggtgc ggcagtcggt gcggtgggca tcggctggct ctcctttaaa
     4681 ctcgggcgca aaaagagcct gatgatcggc gcaatcctgt ttgttgccgg ttcactgttc
     4741 tctgcggctg cgccaaacgt tgaagtactg attctttcgc gcgttctgct ggggctggcg
     4801 gtgggtgtgg cctcttatac cgcaccactg tacctgtcag aaattgcgcc ggaaaaaatt
     4861 cgcggcagta tgatctcgat gtatcagttg atgatcacta tcgggatcct cggtgcttat
     4921 ctttctgata ccgccttcag ctacaccggt gcatggcgct ggatgctggg tgtgattatc
     4981 atcccggcaa ttttgctgct gattggtgtc ttcttcctgc cagacagccc acgttggttt
     5041 gccgccaaac gccgttttgt tgatgccgaa cgcgtgctgc tacgcctgcg tgacaccagc
     5101 gcggaagcga aacgcgaact ggatgaaatc cgtgaaagtt tgcaggttaa acagagtggc
     5161 tgggcgctgt ttaaagagaa tagcaacttc cgccgcgcgg tgttccttgg cgtactgtta
     5221 caggtaatgc agcaattcac cgggatgaac gtcatcatgt attacgcgcc gaaaatcttc
     5281 gaactggcgg gttataccaa caccaccgag caaatgtgga ggacagtgat tgtcggcctg
     5341 accaacgtac ttgccacctt tatcgcaatc ggccttgttg accgctgggg acgtaaacca
     5401 acgctaacgc tgggcttcct ggtgatggct gctggcatgg gcgtactcgg tacaatgatg
     5461 catatcggta tccactctcc gtcggcgcag tatttcgcca tcgccatgct gctgatgttt
     5521 attgtcggtt ttgccatgag tgccggtccg ctgatttggg tactgtgctc cgaaattcag
     5581 ccgctgaaag gccgcgattt tggcatcacc tgctccaccg ccaccaactg gattgccaac
     5641 atgatcgttg gcgcaacgtt cctgaccatg ctcaacacgc tgggtaacgc caacaccttc
     5701 tgggtgtatg cggctctgaa cgtactgttt atcctgctga cattgtggct ggtaccggaa
     5761 accaaacacg tttcgctgga acatattgaa cgtaatctga tgaaaggtcg taaactgcgc
     5821 gaaatcggcg ctcacgatta atctcctcag gcttcctccc gtctcggagg aagccgcctc
     5881 ttgcagtcat cttttcttcg ctctatcctc tgccgctatg aaaacatccc gtctccctat
     5941 agccatccaa caggccgtta tgcgccgcct gcgggaaaaa ctcgcccagg ccaacctgaa
     6001 gctagggcgt aactacccgg agccaaaact ctcttacacc cagcgcggaa cctccgccgg
     6061 aacagcctgg ctggaaagct atgaaatccg cctcaatccc gttttgctgt tggaaaacag
     6121 tgaagctttt attgaagaag tggtaccgca cgaactggca catttgctgg tatggaaaca
     6181 tttcggccgc gtagcgccac atggcaaaga gtggaagtgg atgatggaaa gcgtgctggg
     6241 cgttcccgcc cgtcgtacgc atcagttcga actgcaatcc gtgcgtcgca acaccttccc
     6301 ctaccgctgt aagtgccagg aacatcagct taccgtacgc cgccataatc gcgtagttcg
     6361 tggcgaggcc atctatcgct gtgttcactg cggtgaacag ctggttgcga aataaccacc
     6421 tgaactatca ggaactttcc tgatctggct gattgcatac caaaacaact ttcgctacgt
     6481 tgctggctcg ttttaacacg gagtaagtga tgtaccgtta tttgtctatt gctgcggtgg
     6541 tactgagcgc agcattttcc ggcccgacgt tggccgaagg tatcaatagt ttttctcagg
     6601 cgaaagccgc ggcggtaaaa gtccacgctg atgcgcccgg tacgttttat tgcggatgta
     6661 aaattaactg gcagggcaaa aaaggcgttg ttgatctgca atcgtgcggc tatcaggtgc
     6721 gcaaaaatga aaaccgcgcc agccgcgtag agtgggaaca cgtcgttccc gcctggcaat
     6781 tcggtcacca gcgccagtgc tggcaggacg gtggacgtaa aaactgcgct aaagatccgg
     6841 tctatcgcaa gatggaaagc gatatgcata acctgcagcc gtcagtcggc gaggtgaatg
     6901 gcgatcgcgg caattttatg tacagccagt ggaatggcgg cgaaggccag tacggtcaat
     6961 gcgccatgaa ggtcgatttc aaagaaaaag ctgccgaacc accagcgcgt gcacgcggtg
     7021 ccattgcgcg cacctacttc tatatgcgcg accaatacaa cctgacactc tctcgccagc
     7081 aaacgcagct gttcaacgca tggaacaaga tgtatccggt taccgactgg gagtgcgagc
     7141 gcgatgaacg catcgcgaag gtgcagggca atcataaccc gtatgtgcaa cgcgcttgcc
     7201 aggcgcgaaa gagctaacct acactagcgg gattcttttt gttaacccct accccacgcg
     7261 tacaaccgcg tggggagacg acgcggattt ttaactatgc gtatcccccg catttatcat
     7321 cctgaaccac tgaccagcca ttctcacatc gcgctttgcg aagatgccgc caaccatatc
     7381 gggcgcgtac tgcgcatggg gccggggcag gcgttgcaat tgtttgacgg tagcaaccag
     7441 gtctttgacg ccgaaattac cagcgccagc aaaaaaagcg tggaagtgaa ggtgctggaa
     7501 ggccagatcg acgatcgcga atctccgctg catattcacc tcggtcaggt gatgtcgcgt
     7561 ggtgaaaaaa tggaatttac tatccagaaa tcgatcgaac tcggtgtaag cctcattacg
     7621 ccactttttt ctgagcgctg cggcgttaaa ctggatagtg aacgtctgaa caagaagctt
     7681 cagcagtggc agaagattgc aattgctgcc tgtgagcagt gtggtcgtaa ccgggtgccg
     7741 gaaatccgtc cagcgatgga tctggaagcc tggtgtgcag agcaggatga aggactgaaa
     7801 ctgaatcttc acccgcgcgc cagtaacagc atcaatacgt tgcctttacc ggttgaacgc
     7861 gtccgcctgc tgattggccc ggaaggcggt ttatcggcag atgaaattgc catgactgcc
     7921 cgctatcaat ttactgatat cctgttggga cctcgcgttt tgcgtacaga gacaactgcg
     7981 ctcaccgcca ttaccgcgct acaagtacga tttggcgatt tgggctaacg gagaagaata
     8041 atgatcaagc tcggcatcgt gatggacccc atcgcaaaca tcaacatcaa gaaagattcc
     8101 agtttcgcta tgttgctgga agcacagcgt cgtggttacg aacttcacta tatggagatg
     8161 ggcgatctgt atctgatcaa tggcgaagcc cgcgcccata cccgcacgct gaacgtgaag
     8221 cagaactacg aagagtggtt ttcgttcgtc ggtgaacagg atctgccgct ggccgatctc
     8281 gatgtgatcc tgatgcgtaa agacccgccg tttgataccg agtttatcta cgcgacctat
     8341 attctggaac gtgccgaaga gaaagggacg ctgatcgtta acaagccgca gagcctgcgc
     8401 gactgtaacg agaaactgtt taccgcctgg ttctctgact taacgccaga aacgctggtt
     8461 acgcgcaata aagcgcagct aaaagcgttc tgggagaaac acagcgacat cattcttaag
     8521 ccgctggacg gtatgggcgg cgcgtcgatt ttccgcgtga aagaaggcga tccaaacctc
     8581 ggcgtgattg ccgaaaccct gactgagcat ggcactcgct actgcatggc gcaaaattac
     8641 ctgccagcca ttaaagatgg cgacaaacgc gtgctggtgg tggatggcga gccggtaccg
     8701 tactgcctgg cgcgtattcc gcaggggggc gaaacccgtg gcaatctggc tgccggtggt
     8761 cgcggtgaac ctcgtccgct gacggaaagt gactggaaaa tcgcccgtca gatcgggccg
     8821 acgctgaaag aaaaagggct gatttttgtt ggtctggata tcatcggcga ccgtctgact
     8881 gaaattaacg tcaccagccc aacctgtatt cgtgagattg aagcagagtt tccggtgtcg
     8941 atcaccggaa tgttaatgga tgccatcgaa gcacgtttac agcagcagta acccacctta
     9001 gcgagaagga tctcgttgag actctgagtg acagcgccct tctttccacg catactgggc
     9061 gctgttgctt ttttgaacca ggaaacagaa cctctgacaa tgaatttaca gcatcacttt
     9121 cttattgcca tgcctgctct ccaggatccg attttccgtc gttccgtggt ctacatttgc
     9181 gaacataata ccaatggtgc aatggggatc atcgtcaaca agccgctgga aaatctcaaa
     9241 attgaaggga ttctggaaaa gctgaagatc acgccggagc cgcgtgatga atcaatccgt
     9301 ctggataaac cggttatgct cggcggccca ctggctgaag atcgcgggtt tattttgcat
     9361 actccgccct ccaattttgc ttccagcatt cgcatttcag acaacacggt gatgaccact
     9421 tcccgcgatg tgctggaaac gctcggcacc gataaacaac cgtctgacgt attggtggct
     9481 ctgggctatg cctcctggga aaaaggtcaa ctggaacaag aaattctcga taacgcgtgg
     9541 ctaacggccc cggcggatct gaatattctg ttcaaaacgc cgattgccga ccgctggcgc
     9601 gaggcggcaa aactgattgg tgtggatatt ctcaccatgc ctggtgtggc aggacacgcc
     9661 tgatgagtgg aaccttactc gccttcgact tcggcaccaa aagcattggc gtagcggtcg
     9721 gccaacgcat taccggcacc gctcgccctt tgcctgcaat taaagcgcag gacggtacgc
     9781 cggactggaa cctcatcgaa cgtttactga aagagtggca gcctgacgaa atcatcgtcg
     9841 gtttgccgct gaatatggac ggcaccgaac agccgttaac cgccagagcg cgcaaatttg
     9901 ccaaccgtat tcatggtcgt ttcggtgttg aagtaaagct ccatgacgag cgtcttagca
     9961 ctgtggaagc ccgttccggt ctttttgaac agggcggcta tcgggcgctc aacaaaggca
    10021 aagttgactc tgcctctgcg gttattattc tcgaaagcta tttcgagcag ggatattaag
    10081 gcgatctaaa agcctggcgg atagtcgtta ccgccatctt gccggatgcg gcgtgaacgc
    10141 cttatccagc acacatctgg cagcggctgt aggtctgata agacgcgtta gcgtcgcatc
    10201 agacatttat tgcctttata ggccctataa acttgcgcat cgggcatggc aacgtcacaa
    10261 acgcccttcc cccacccgct gctgataact ctgctgaaac gttaacatcc ccacctgctg
    10321 cccggtttga ataacatgcg gtaactggtg ggttttccct tcgcgaatca aattccccac
    10381 cgcgggagtg ttaatcagta attcaaacag cgccacgcgt ccttcctgtt tatccacttc
    10441 cagcttttgt gacagcactg cccgtaaact acccgcaagt tgattacgta cgggatcttt
    10501 ttcctgcgcc ggaaatgaat ccaccagtcg ctcaactgcc tgcgcggcac cacgtgtatg
    10561 taaggttgcc agcaccagat gcccggtttc tgccgccgtc agcgccagac ggattgtctc
    10621 actgtcacgt agctctccga gcaaaatcac atcaggatct tcccgcaatg cagcccgcaa
    10681 ccccgatgcg aacgtcatac agtgcaaacc aatttcccgc tgctggatca aacatcgctg
    10741 gctggcatag agatattcca caggatcttc cagcgtcaga atatgcgcat cggcatgttg
    10801 attaagatag ccaaccatcg ccgccagcgt ggtagatttg ccactccccg tagcccccgt
    10861 caccagaatc aggccattct cgctcttgag taattccggc aataccggtg gcgcaccaag
    10921 ctgttcgagc tgtggacagt gcgaaggtaa caaccgtaat gccagagaaa taccttgccg
    10981 ttgcgcgaac gcactgccac gcaaccgctg gttttccgcc agcgacacgg caaaatccag
    11041 ctggccattt tccagcaata ttgcccgctg atcgtcatcc agccactccc gcagtagctc
    11101 ttcgacgtcc ggcgcatcaa acggcgcagc ttccattctg ccacgaatgc gccatcgtgc
    11161 gggccaggcg ctgcacaggt gtagatccga gacgttatgc tttacactaa gggccacaat
    11221 ttcttccata ttcatactaa gatcctcgga aaatgaacga tattgcgcat aacctggcac
    11281 aggtccggga caaaatctca gcggctgcaa cgcgttgcgg ccgttctcca gaagaaatta
    11341 cgctgcttgc agtcagtaaa acaaaacctg cgagcgccat cgcagaagcc attgatgccg
    11401 ggcagcgtca atttggtgaa aactacgttc aggaaggggt agataaaatt cgccactttc
    11461 aggaactggg cgtaacagga ttagaatggc attttattgg cccgttgcag tctaataaaa
    11521 gccgcctagt ggcagagcat ttcgactggt gtcataccat cgaccgtttg cgcattgcta
    11581 cccgcctcaa cgatcagcgc ccggcagaac ttccccctct taacgtactg attcaaatta
    11641 atattagtga tgaaaacagt aagtccggga ttcaactggc agaactggac gagctggcag
    11701 cggcggtcgc tgaactaccg cgtttacgtc tgcgcgggct gatggcaatc cctgcgcctg
    11761 agtcagaata tgtaaggcag tttgaagttg cacgccaaat ggctgtagca tttgccggac
    11821 tgaaaacgcg ctacccgcat atcgacacgc tctctctggg aatgtcggac gatatggaag
    11881 ccgccattgc ggcaggtagc acgatggttc gtatcggcac tgcaattttt ggtgcgcgtg
    11941 attactctaa aaaataagga attaaaggaa cgccatgaat acgttgactt tcctgctttc
    12001 aacggtcatt gagctgtata ccatggtgct gttattacgc atctggatgc agtgggctca
    12061 ttgtgatttt tacaacccct tctcacagtt tgtagtgaag gtagcgcagc caattatcgg
    12121 gccactgcgc cgcgttattc cggcaatggg tcccattgac agcgcctcgc tgctggttgc
    12181 ctatattctc agttttatca aagccattgt gctgtttgaa gtggtgacct tcctgccaat
    12241 catctggatt gccggtttac tgattctgct gaaaaccatc ggcttgctga ttttctgggt
    12301 cctgctggtg atggcgatta tgagctgggt aagccagggt cgtagcccga ttgaatacgt
    12361 gctgattcag ctggccgatc cgctgctgcg cccgattcgc cgcttgctac cggcaatggg
    12421 tgggattgat ttctcgccga tgatcctcgt tctgctgctg tatgtcatca atatgggtgt
    12481 cgcagaagta ttacaggcaa ccggaaacat gctgctgccg gggctgtgga tggcgttatg
    12541 agtgccgtaa cagttaatga tgacggtctg gttttacggc tctatattca gccgaaagcc
    12601 agccgtgatt ctattgtcgg tttacatggc gacgaagtta aagtcgccat taccgcgccg
    12661 ccggttgacg gccaggccaa caatcatctg gtgaagtttc tcggtaagca attccgggtt
    12721 gccaaaagcc aggtggtgat tgaaaaaggc gaacttggcc gccacaaaca aattaaaatc
    12781 attaatccgc aacaaatccc gccagaaatc gcggcgttaa ttaattaggt atcctatgca
    12841 aaaagttgtc ctcgcaaccg gcaatgccgg taaagtgcgt gagctggcgt cgctgcttag
    12901 cgactttggt cttgatatcg tggcccaaac agacctcggc gttgattccg ctgaagaaac
    12961 cggcctaacc tttatcgaaa acgcgattct gaaagcgcgc catgcggcaa aagtgaccgg
    13021 tttaccggca attgccgacg actctggtct ggcggtagat gcgctgggtg gcgcgccggg
    13081 tatttactcc gcgcgttatt ccggtgaaga cgcgaccgat caaaagaatc tgcaaaaact
    13141 gttggaaaca ctgaaagacg taccggacga ccaacgtcag gcgcgtttcc actgcgtgct
    13201 ggtatatctg cgtcacgcgg aagatccgac tccgctggta tgccacggca gctggccggg
    13261 cgtgattact cacgaaccag cgggcactgg tggctttggt tatgatccaa tcttcttcgt
    13321 accttccgaa gggaaaaccg ccgccgaact gacccgcgaa gaaaagagcg ccatttccca
    13381 ccgtggtcag gcgttgaaac tgctgctgga cgctttacgt aatggttaaa ttaccgccgc
    13441 tgagtctcta cattcacatc ccgtggtgcg tgcagaaatg cccgtactgc gatttcaact
    13501 ctcacgcgtt gaaaggagaa gtgccgcacg acgattatgt tcagcatctg ctttgcgatc
    13561 tggacaatga tgtggcttac gctcagggcc gtgaagtaaa gacaatcttt attggcggtg
    13621 gtacgccgag cctgctttcc ggcccggcga tgcaaacgct gctagacggc gtgcgttcgc
    13681 gtttgccgct ggcagcggat gcagaaatta ctatggaagc gaacccaggc acggtagaag
    13741 cggatcgctt tgtcgattat cagcgtgctg gtgtaaaccg catctctatt ggtgtgcaga
    13801 gttttagcga agaaaagctg aaacgacttg ggcgtattca tggcccgcaa gaagcgaaac
    13861 gagcggcaaa gctggcgagc ggtttagggt tacgtagctt taaccttgat ttgatgcatg
    13921 gactaccgga tcaatcactg gaagaggcgc ttggcgatct acgccaggcc attgaactga
    13981 atccgccgca tctttcctgg tatcaactga ccatcgaacc taatacgctg tttggctcgc
    14041 gaccgccggt gctgccggac gatgacgcgc tgtgggatat tttcgaacag gggcatcagt
    14101 tattaaccgc agcgggttat cagcaatatg aaacttccgc ttacgccaaa cccggttatc
    14161 agtgccagca caatctcaac tactggcgct ttggtgacta catcggtatt ggctgcggcg
    14221 cgcacggcaa agtgaccttc ccggatgggc gcattctgcg taccactaaa acgcgtcatc
    14281 ctcgtggttt tatgcaagga aggtatctgg aaagccagcg tgatgtcgaa gccgcagata
    14341 agccgtttga gttctttatg aatcgcttcc gtctgctgga ggccgcgccg cgcgtggagt
    14401 ttattgcgta taccgggctt tgcgaagatg tgattcgccc acagttagac gaggcgattg
    14461 cccagggtta tctcaccgaa tgtgcggatt actggcagat aacggaacat ggtaaattat
    14521 tcttaaactc acttcttgaa ttattcctcg ctgagtaatc attaatgcgc ctgcgggcgc
    14581 attaatcact tcttttatcc ccccgttaat taacctcggc agaaatatat ttcttactga
    14641 ataaatatcg acgacattta ttatatatat ttagtgaata tttactaatc attcattgat
    14701 tataaatatg tacaacctgg cgcacaaatt taatatttaa tttctgactt tatttcaagt
    14761 tgtgataaac atagacaagt tgtcagacag gttaaccaat ttgtcgcaca aagcactgat
    14821 aagcacaatt agatatcaac caatttccat ccttcaacag ggagtttttt tatgaaaatg
    14881 aggtcattta cccgctcatt agtctgcgca tccttgctgg ctctggttag cactggcgta
    14941 aacgccgcag aaaaagtgac gttaaagtta gcacacaacc tggaaagaag tcatgttgta
    15001 catcagagtt ttgaagagct ggcgaaagag gtaaaacagc tgtcaaaagg taatatggtg
    15061 atccgtattt atcccagcag ccagatgggt aatgcgcgag aaactatgga attgttgcaa
    15121 aatggcgcac tggatatgac caaaggttcc gccagtgatc tggagtcttt tgataatatt
    15181 tatgctatct ataatttgcc gttcttatta agttgttttt ggtgaagttg gtaaagaaat
    15241 tatggactca acaaaagaca aaggcttttt tgctttatca gcttatgtag cgggtactcg
    15301 cagtttctat gcgaaaaaac cgattacaaa gccagaagat cttaaaggac tgaaaattcg
    15361 tgttcagcca agtccgacca ccatcaaaat gattgaactg atgggtggtt cacccactcc
    15421 aatttcattt ggcgaagttt atactgcgat gcagcaaggt gtagtagatg gcgcagaaaa
    15481 taacgtgcct tcctggatgc agactcgaca tattgaaatt gccaaagttt tttctgaaga
    15541 tgaacacgcc tcaattcctg actttttggt tatctccacc aaaacatgga ataaattacc
    15601 cccagaacag caacaaatcc tcgaaacagc ggcgaagaaa tccgaagctt atcaacaaaa
    15661 attgtgggaa aaaattgacg ctgacacacg tgctcaggcg aaagctatgg ggggtgaaat
    15721 tgtaaaagtg gataaagcgc cattccgtaa agccgtgcaa cctctgtttg acgacttcaa
    15781 aaaagatcca aaacaagctg ctttgttaga gaaattcgac aacgctgctc aataatattc
    15841 cgggccagcc tctggccctg attttccagg atgcttacaa tgatatttaa ccgcctgaaa
    15901 cttgcggtag accgcgtcat tgccgcgttt tctgtcgccg taatgcttgc tctggttgta
    15961 tgcgtagtct ggcaagtatt tagccgctac gtacttaacc agccaagtac attaaccgat
    16021 gagctggcac gctttttgat gatctgggta ggattattag gtgctgcata taccgtcggc
    16081 gcgcagcgac atctctctat tgatttattt gctctcgccc tgaataagcg caaacaatta
    16141 cttcttagta tagtcattaa cgttctcatt ttaggttttg ccggttcggt cattgtgact
    16201 ggtggcctga aattaattga taaaacactg gcaacatcac aggtatctgc cgctatgcaa
    16261 ataccgatgg gatacgtata tatcattctg ccattaagtg gagtggtaat gatgttttat
    16321 gc
//
LOCUS       DADMGC010000088        16220 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.088, whole genome shotgun sequence.
ACCESSION   DADMGC010000088 DADMGC010000000
VERSION     DADMGC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16220
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.088"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..880)
                     /locus_tag="JNZ73_002767"
     CDS             complement(<1..880)
                     /locus_tag="JNZ73_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY8732995.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAG"
     gene            987..1283
                     /locus_tag="JNZ73_002768"
     CDS             987..1283
                     /locus_tag="JNZ73_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8732996.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1417..1842)
                     /gene="arsC"
                     /locus_tag="JNZ73_002769"
     CDS             complement(1417..1842)
                     /gene="arsC"
                     /locus_tag="JNZ73_002769"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HAY8732997.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1855..3144)
                     /gene="arsB"
                     /locus_tag="JNZ73_002770"
     CDS             complement(1855..3144)
                     /gene="arsB"
                     /locus_tag="JNZ73_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HAY8732998.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3198..3551)
                     /gene="arsR"
                     /locus_tag="JNZ73_002771"
     CDS             complement(3198..3551)
                     /gene="arsR"
                     /locus_tag="JNZ73_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HAY8732999.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            4296..4379
                     /locus_tag="JNZ73_002772"
     CDS             4296..4379
                     /locus_tag="JNZ73_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HAY8733000.1"
                     /translation="MIDKAIIVLGALIVLLELIRFLLQLLN"
     gene            complement(4433..5785)
                     /gene="gorA"
                     /locus_tag="JNZ73_002773"
     CDS             complement(4433..5785)
                     /gene="gorA"
                     /locus_tag="JNZ73_002773"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAY8733001.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5857..6699)
                     /locus_tag="JNZ73_002774"
     CDS             complement(5857..6699)
                     /locus_tag="JNZ73_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HAY8733002.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6902..8944
                     /gene="prlC"
                     /locus_tag="JNZ73_002775"
     CDS             6902..8944
                     /gene="prlC"
                     /locus_tag="JNZ73_002775"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HAY8733003.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRSGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8952..9704
                     /gene="rsmJ"
                     /locus_tag="JNZ73_002776"
     CDS             8952..9704
                     /gene="rsmJ"
                     /locus_tag="JNZ73_002776"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HAY8733004.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9753..11222)
                     /gene="dtpB"
                     /locus_tag="JNZ73_002777"
                     /pseudo
     CDS             complement(9753..11222)
                     /gene="dtpB"
                     /locus_tag="JNZ73_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(11528..11962)
                     /gene="uspA"
                     /locus_tag="JNZ73_002778"
     CDS             complement(11528..11962)
                     /gene="uspA"
                     /locus_tag="JNZ73_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HAY8733005.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12353..12688
                     /gene="uspB"
                     /locus_tag="JNZ73_002779"
     CDS             12353..12688
                     /gene="uspB"
                     /locus_tag="JNZ73_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HAY8733006.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12759..14258)
                     /gene="pitA"
                     /locus_tag="JNZ73_002780"
     CDS             complement(12759..14258)
                     /gene="pitA"
                     /locus_tag="JNZ73_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HAY8733007.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            14490..15689
                     /locus_tag="JNZ73_002781"
     CDS             14490..15689
                     /locus_tag="JNZ73_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY8733008.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPVLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITT
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         4102 a         4105 c         4216 g         3797 t
ORIGIN      
        1 cgccggcact gccgatgatt aatgccatta atgctcccag acccattcct tttgtcatca
       61 gaacagatgc cagagggata accgcctcag cccggatata cagcggaata ccgacaacgg
      121 cgcttaatgg gatggcgaag ggattatctg ctcctgcatg agcggcaatc cacgctgagg
      181 gaataaagcc ataaataaaa gatcctatta atactcctaa caaaagatat ggcagtacat
      241 ctttaaactg ttgcaacgca tctttggtgg ctagttttat gggattaata cttactacag
      301 ctttggtact gcaacaggta actacggggt tctcaatggc tttagccgct ggactacaac
      361 aactcacttt tatgggcgta tatgtcgttc ccggcgaagt ttttgctgga gcacaacaat
      421 ttgctgatga gcttttactg gcaatgatat gacgttcaaa ccccagagaa tccaggataa
      481 tactggcaag aacggagacg ccggctgcga taatcgcgta caacaaggtg actttccaac
      541 caaaggtcat ccacattagt ccgacgataa tgggattaag taatggtgaa acaaataaaa
      601 aagtgagagt cggaccaaac ccggctttcg ctgacaacaa tccacgtaac atgggtattg
      661 tcgagcaact acagaacggg gtaacggctc ccaacagagc agccaggaga tagcctctcc
      721 cttttctggc ccccatcatc tgctggattt tatggtctgg caccttttgt cttatcaggc
      781 tgacaccggc actaatcact ataaacagca acgaaagctc gacggcgaga aacacaaaca
      841 tctctgcggc atcttgcagc atcgcaagcc aactactcat acgtaacctc attgggtaaa
      901 tctatatttc cagaataatc gaaatgaaat tcagatcaag atatttctgg tattatcgaa
      961 atatcaacca gacagagggg tgtcatatgc agttggaaga agtcgctaaa gcgctgaaag
     1021 agctaggtca cccgacccgt ttgttcatat ttaaacatct tgtcaaagcc ggggaacaag
     1081 ggttgccggt cggtgagttg caaaagcagc ttgggatacc tggctccaca ctcagccacc
     1141 atatatcagc actggtatcg gtaggactgg tcaaacaaaa ccgtgagagt cggacgctga
     1201 tgtgtgtttc gcaatacgca atgctggaag cgataattga attcttacgc gaggagtgct
     1261 gcgtaaatag caaaattgcc tgatgcgcta cgctctacat atttcatgcc atatattgaa
     1321 tttgcacggt tttgtaggtc ggataagacg cgttagcgtc gcatccgaca taaacaaagc
     1381 gcactttacc aactatcggc agtggatatc gccgctttat ttcaggcgct tacccgcttc
     1441 atcaaccact ttctcgccat cttccttgga gaatgcgcct ttttgcgcat ctggcagaat
     1501 ttccagcacc acttctgaag ggcggcacag gcgagttccc agcggcgtca ccacaatcgg
     1561 gcgattaatc agaatcgggt gctgaagcat aaagtcgatt aaccgatcgt cagtaaattt
     1621 atcttccgca aggcccagct cctcatacgg ttcgacgttt ttacgcagca gcgcgcgtac
     1681 ggaaatcccc atatcggcaa tgagtttgac cagttcatcg cgcgttggcg gagtttccag
     1741 ataatggata atggtcggtt ctgtgccgct gttgcggatc atctccagcg tattacgcga
     1801 cgtgccgcag gccgggttgt gataaatggt aatgttgctc atatcagtat ctcattacaa
     1861 agtgaaagag agacgtagcg ccagcgcagc cagcgtcaca aacagcacag gcagggtcat
     1921 gataatccct gtacggaagt aatatcccca gctgatagtc atattcttct gcgaaagtac
     1981 gtgcagccag agcagcgttg ccaggctacc aatcggggta attttcggcc ccaaatcgca
     2041 gccaatcacg ttggcataaa ccatcgcttc tttgataacg ccagatgccg tgctgccatc
     2101 aatggacaac gcgccaacca gtaccgtcgg catattgttc ataatagaag agaggaaggc
     2161 ggtgaggaat ccggtgccga gcgtcgcggc ccacaggccg ttatccgcca gcacgttgag
     2221 tacgccagaa agatattccg ttaatccggc attgcgcagg ccataaacca ccagatacat
     2281 gccgagcgag aagatgacaa tctgccaggg ggcaccgcgc aggactttac ccgtattaat
     2341 cgcatgaccg cgtttagcga cgacaaataa tatcagtgcg cccacagctg caatggcgct
     2401 caccggaatg ccgagcggtt ccaggacgaa aaatcccacc agcagaagca gtaaaacaac
     2461 ccagccagtt ttgaacgtag caggatcttt gatcgcttct gcgggagatt tcagcagcgc
     2521 catatcgtag ttctgcggaa tatctttgcg aaaatagaga tgtagcatca ccagcgtggc
     2581 aacaatcgcg gcgatatcca ccggcaccat caccgaggcg tattcgcgaa agccgaggcc
     2641 aaagaaatca gcggaaacga tattcaccaa gttggagaca ataagcggca ggctggcggt
     2701 atcggcaatg aatccggccg ccatcacgaa cgccagcgtc gtgcctttac tgaatcctaa
     2761 agccagcagc atggcgatga caatcggcgt caaaataagc gccgcgccat cattggcaaa
     2821 cagggccgca acggcggcac cgagcaggac aatccaggta aacagcaagc gaccgcgtcc
     2881 attgccccag cgtgaaacgt gcagcgctgc ccattcaaaa aagccggact catccagcag
     2941 caggctgatg ataatgacgg caataaacgc cgccgtcgcg ttccagacga tattccacac
     3001 caccggaata tcacccggat gaaccacgcc cgtaaccaac gccagtaccg cgccgagcgt
     3061 tgcactccag ccgatgccta aacctttcgg ctgccagata accaatacga tggtcaggac
     3121 aaagatagcg cctgccagta acataatgcc tcccggataa aacacatgtg caaatccata
     3181 tgtgtttagc taaattttta actgcaaatg ttcttactgt ctccggaaca gttttgtctg
     3241 gccaggttgc ggacaattgc ctgaatcttt tcctgttcac atcgccaggc ttcatcaata
     3301 attttcgccg cccatgctgg aatatgcggc gataagcggt aatgaaccca cttaccttgc
     3361 ttgcggtcca gcaatagccc gctttcacgc agcaatgcca gatggcggga tatcttgggc
     3421 tgcgactgat cgagggcagt gcagagatcg cagacgcata actctcccag ttcgctaagc
     3481 agtaaaacga tgcccagacg ggtttcatca gcaagaattt tgaacaattg gatgggtaac
     3541 agaaatgaca tattgcgctc ctgattgttg caggtagtgt ctctcttcga agcggataag
     3601 tcaaaaacat atatgactta acgaatgtgt aagtgcagag gaaggtaata ggtgtgaatt
     3661 ttgagttggc tattcatttg aaaggcggtt tgaatcagga cgtaaaaaaa accgactttg
     3721 cgtcggtttt tttactcttc agcctgagtt ggtggctctg gctggagtga gaatgcccac
     3781 atctaacggt tcaatactat tagccagtgg ctaacattgc aagcgttagc gatttcttca
     3841 gctggagaag ggacagtttg ctatacatgg tggtgaaaga ggatttatat actttcatcg
     3901 ggtcgctgcc gtaccaagcc ttgtccgaat ccggacgtaa aaaaaccgac ttcgcgtcgg
     3961 tttttttact cttcagccca gagttggtgg ctctggctgg agtgagaatg ccatcatcta
     4021 acgcatcaat gctaatacca tactgaaact attgcaaggt tatgctggtt atttagccta
     4081 attacactgt atgattatcc agttagctct gaggcatttt cactctggca atgcgcataa
     4141 acgctttcaa agtcctggtc agaagtacgg gtggtgccgt taactgatgc tctggccgga
     4201 gtgagattgc ccacatctaa caatgaggca tgcgccgtga caggcagtgg atgagtaagc
     4261 ggatgcattc tcactccatc gcatggagaa aacgggtgat tgataaagca atcatcgttc
     4321 ttggggcgtt aattgtgctg ctggaactga tccgctttct gcttcagctt ctgaactgat
     4381 agcggaaacg taattaaggg ctaagagcac actactctta gccctttaac atttaacgca
     4441 tggtcacgaa ctcttctgcc gccgttgggt gaatggcgac ggtattgtcg aagtcttttt
     4501 tggttgcgcc catcttcagc gccaccgcga agccctgcaa catttcgtcc ataccaaagc
     4561 caatgccgtg aataccgaca atcttctctt ctggcccaac gcacaccagc ttcatgcggc
     4621 acggctggcg gtgagtggtg acggcggtat acatcgcggt gaaagaggat ttatacactt
     4681 tcacctgatc gtcgccgtac tgctcgcgcg cctgcggttc cgttaaacca acagtaccaa
     4741 tcggcggatg gctgaagacc acggtcggaa tgttgctgta atccagatgc tcatccggct
     4801 tgttattaaa caagcgttca gagagacggc gacccgctgc aactgccacc ggtgtcagtt
     4861 ccactgcacc cgtgttatcg cccaccgcgt aaataccttc aatattggtg ttttgatatt
     4921 tatcgacgac gatatagcct ttttcgttgg ttttaacgcc agcggcttcg aggttgatgt
     4981 tgtcattggc aggctcgcga ccaatcgccc aaatcaggca atccaccgtt tcactgcgac
     5041 catcttccag ctccagcgtc aggctaccat cggcattttt cactaccgct ttcgggatgg
     5101 cgttggtatg caactgtggg ccttcggcgt tcatcacttc gaccagcgtt tcagagatca
     5161 tcgggtcgaa gctgcgcagc ggcgcatgtt tacgcacaaa cagatgcgtt ttcgcgccga
     5221 ggccgttaat cacgccagcc agctcaacgg cgatgtaacc cgcgccaaca accgccacgc
     5281 gctctggcaa agcaggaagg gcgaagaagc catcggaatc aataccgtat tccacgcctg
     5341 gaatatccgg gtggctcgga cgaccgccgg tggcgatcag aatatgatcg gccgtgatgg
     5401 tttcgccgtt aacttccagc gttttggcat caacgaagcg ggcaaagcct ttgattacat
     5461 caacgttatt tttaccgagc acgttttcat aggaagtatg aatacggtcg atataggcgg
     5521 tacggctggc gatcaacgtt tcccagttga acttgttgat ggtggtatcg aaaccataat
     5581 cagggccgta catatggatc gcttcacgga tttgcgccgc gtgccacatc acttttttcg
     5641 gcacgcagcc gacgttcacg caggtgccgc ccagctcttt ggcttcaatc agtgcgcatt
     5701 tctggccgta catagccgcg cggttgatgg aggcgatacc gccgctgccg ccgccgatgg
     5761 cgatgtaatc atagtgttta gtcatgacat attgtcctta tcgttgatta ccgcgattgt
     5821 agcgcgagac gtaataggtg ccagcaatgg ctgcaattac tccggcacga tccagcttac
     5881 ggtggcgtgc ccggtacctg ccggaaccag tttgctgtgc agccacgaca gcacgttatt
     5941 catctgctgt tccagcttcc acggcgggtt aatcacaatc atgccggaag cggtcatgcc
     6001 acggcgatcg ctgtctggca gcaccgccag ttcaatttgc agaattttgc gaatgccggt
     6061 cgcttccagg tcgtggatca tgcgcttaat ttgctggcgc agcaccaccg gataccacaa
     6121 tgcgtaagtg ccagtggcga aacgtttgta accttctgct atcccgctga ccaccgcctg
     6181 atagtcggtt ttcatttcat acggcgggtc gataaggatt aaaccacggc gagaaaccgg
     6241 cggcagtttg gccttaagtt gctggaaacc gtcggctttt tcgacgcgtg cacggctgtc
     6301 tttctgaaat ccagaacgca gcaacgggta atcgctcggg tgcagttcgg tcagttgcag
     6361 gctgtcttgt tcgcgcagta gctggcgagc aatcaacggc gaaccggggt agtaacgcaa
     6421 ctggccgcta cggttgaagt gttttaccac attgatgtac gcctccagtt ctgcgggcaa
     6481 atcgtcctgc tgccagatac gggcgatacc ttcgagatat tcgccggtac gctcggcatg
     6541 ttcgctacct aactgataac gcccggcccc tgcgtgggtg tcgagataga gaaacggttt
     6601 atctttctct ttcagcgact cgatgatcag gctctgaacg gtatgtttaa ggacgtcggc
     6661 gtggttgcca gcgtgaaagc tgtggcgata actgagcatg ggtaaaggtg ttccggtaag
     6721 taaaaattgg cccgtattcg gggcgcacaa aagcctatca ggacagtata ccgaaaagag
     6781 gccgccgccg cgaaagcgta acgtttccca ttgaaattca ctacacttaa ccccatgcta
     6841 cacacattat gtaaagcgcc tgttgagcgc ttccttaacc tctttaacca ggactgcgct
     6901 aatgacgaat ccgttactga ctccctttga attgcctccg ttttctaaaa ttctcccgga
     6961 acatgtcgtt ccagccgtga ctaaggcatt gaacgactgc cgcgaaaatg tggagcgcgt
     7021 agtagcgcaa ggggcaccgt acacctggga aaatctctgc cagccgttgg cggaagtgga
     7081 cgatgttttg gggcgtatct tctccccggt cagccacctg aactcggtga aaaatagccc
     7141 ggaactgcgt gaagcgtacg aacaaaccct gccgctgctg tcggaataca gcacctgggt
     7201 agggcaacat gaagggctgt ataaagcgta ccgcgacctg cgcgatggcg atcattacgc
     7261 cacgctgaac acggcgcaga agaaagcggt tgataacgcc ctgcgtgatt ttgaactctc
     7321 tggcataggt ctgccgaaag agaaacagca gcgttacggc gaaattgcta cccgtctttc
     7381 tgaactgggc aaccagtaca gcaacaacgt cctcgatgcg acgatgggct ggaccaaact
     7441 cgttaccgac gaagcggggc tggcggggat gccagaaagc gcgctggctg cggcaaaagc
     7501 ccaggccgaa gcgaaagagc tggaaggcta cctgctgacg ctggatatcc caagctacct
     7561 gccggtaatg acctactgcg acaaccaggc tctgcgcgaa gagatgtatc gcgcttacag
     7621 cacccgcgcc tccgatcaag gcccgaacgc cggtaagtgg gataacagca aggtgatgga
     7681 agagatcctc gcgctgcgtc acgaactggc gcaactgctg ggctttgaaa actacgcctt
     7741 taaatccctt gccactaaaa tggcagaaaa cccgcagcag gtgctggatt tcttaaccga
     7801 tctggcaaaa cgcgcgcgtc cgcaaggcga aaaagagctg gagcaactgc gtgccttcgc
     7861 taaagccgaa tttggtgtcg atgagttgca gccgtgggat atcgcttact acagcgaaaa
     7921 acagaaacag cacctctaca gcatcagcga cgagcagctg cgtccgtact tcccggaaaa
     7981 caaagcggtt aacggcctgt ttgaagtggt gaaacgtatt tacggcatca ccgctaaaga
     8041 gcgtaaagat gttgatgtct ggcatccgga tgtacgtttc ttcgaactgt atgacgaaaa
     8101 taacgaactg cgcggcagct tctacctcga tctgtatgcc cgtgaaaaca agcgcagcgg
     8161 ggcgtggatg gatggctgcg taggccagat gcgtaaagct gatggttctc tgcaaaaacc
     8221 ggtcgcgtat ttgacttgta acttcaaccg cccggtaaat ggtaaaccgg cgctgttcac
     8281 tcacgacgaa gtgatcaccc tgttccacga gttcggtcac ggcctgcacc atatgctgac
     8341 ccgcatcgaa accgctggtg tttccggtat cagcggtgtg ccgtgggatg cggtcgaact
     8401 gccgagtcag tttatggaaa actggtgctg ggagccggag gcgctggcgt ttatctctgg
     8461 tcactatgaa accggcgaac cgctgccgaa agagttgctg gataaaatgc tggcggcgaa
     8521 gaactaccag gcggcgctgt ttattctgcg tcagctggag ttcggcctgt ttgatttccg
     8581 ccttcatgcc gagttccgcc cggatcaggg ggcaaaaatc ctcgaaactc tggcagaaat
     8641 caagaaactg gttgccgtgg tgccatctcc gtcctggggc cgtttcccgc acgctttcag
     8701 ccatattttc gccggtggtt atgcggcagg ttactacagc tacctgtggg ctgacgtact
     8761 ggcggcagat gctttctcgc gctttgagga agagggcatt ttcaaccgtg aaaccgggca
     8821 gtcgttcctc gacaacattc tgagccgtgg cggttcagaa gagccgatgg atctgttcaa
     8881 acgcttccgt ggtcgtgaac cgcagctgga tgcgatgctg gagcattacg gcattaaggg
     8941 ctgatcattc agtgaaaatc tgcttaattg atgaaacagg cgccggagac ggcgccttat
     9001 ctgttctggc ggcccgctgg gggctggagc acgatgaaga caacctgatg gcgctggtgt
     9061 taacgccgga acatctggaa ttgcgcaagc gtgatgagcc aaaacttggc ggcatctttg
     9121 ttgattttgt tggcggagcg atggcgcacc gacgcaaatt cggcggtggt cgcggtgagg
     9181 cggtggcgaa agcggtgggc attaaaggcg attatttgcc ggatgtggtg gacgccaccg
     9241 cagggctggg gcgcgatgcc tttgtactgg cttcagtcgg ctgccgcgtg cggatgctgg
     9301 agcgtaatcc agtggttgcc gcgctactcg acgacggcct ggcgcgtggt tatgcggatg
     9361 cggaaatcgg cggctggttg caggagcggt tgcagttaat tcacgcctcc agcctgacgg
     9421 cgctaaccga tattacccca cgcccgcagg tggtctatct cgatccgatg ttcccgcata
     9481 agcagaaaag cgcgctggtg aaaaaagaga tgcgtgtctt tcagtcgctg gtggggccgg
     9541 atcttgatgc cgatggatta ctggagcctg cacgcttgct ggcgaccaaa cgcgtggtgg
     9601 taaagcgccc ggactacgcg ccaccgctgg cgaatgtcgc cacgccaaac gcggttgtga
     9661 ctaaagggca tcgctttgat atctatgcag gtacgccagt gtaaaagtaa agcagggtag
     9721 cgataacgct accctgcttt ctgcaagaat aattaatggc tttccggcgt cgcaatcatg
     9781 cgtttcagcc acggcaccat caacagcatc actactgcaa cgcccagcgt gaccagacca
     9841 atcttaccaa acacgttggt atagacgggc aacgtctcaa gcggatcggt aatgttgtcc
     9901 ggcaccgcgg taaatgttgc cacatagccg cccagcaaga acgcggcagc ctgcgtcagg
     9961 aaccacatcc cgagaataaa gcccatcaaa tgctgcggca ccagggcagc aatcatcgcc
    10021 aggccaaggg cgctaataaa caattcaccc aggctctaga ataaatacac cagcacgata
    10081 aaccatggcg atgtcagccc ctggtcatcc gcaaaccaca ttcccgcagc agcagtcgtc
    10141 aaaaagccca gtgagcacat aaacatgccg agagtaaatt tcatcggcat cgagaggtct
    10201 ttgcctttgt tacccagatg cgtgtaaatg cctgccagta ttgggctggc gagtaccacc
    10261 cagaacgggt taagcgcctg gaagctgacc gggttgatgg aaaaaccgag aatttcatga
    10321 tgcacgttgt tgatggcaaa gaagttcagc gatgttggca tctgggcgta gagaatgtaa
    10381 aacaccaccg cttcgagcat caggacaaag gcgacaaaca ttttattgcg cccggtttta
    10441 tccagcttga atgcctgacg aaagaagatg atggtgacga cgatggagag aacaatcagc
    10501 accagattgg cgacttctac gttgtgcatc agccatgcac atacgaagat catcaccacg
    10561 ctgccaagta atacgtacaa cagtttgctg aagctcatcg gcttgaagtc gggttcagaa
    10621 ccaatgtctt tcaccattcc acgacaggcg atgtaaacca gtaatgcgat aattaacccc
    10681 gccccgcaca ggttgtaggt gactgaataa ccgaatctat cagcgatcac aggggccagc
    10741 gataacgcta tcaacgagcc gatattgatc gacatataga acagggtgaa tgcgccatca
    10801 agccgcggat ctttcggcgg atagcacttc gaaagcaagc tggctgggtt agctttaaac
    10861 aggccgttac cgacagcgat agtccccagg gcgatgaaaa tcaggtcagg cttaagtagc
    10921 gacatgccgg tcatgaagta gccaatcgcc agcacaagtg ctccaagaac aatggtgcgt
    10981 ttggtcccca acaggtggtc gccgacatag ccgccaatgg aaatgaggcc atagaccagt
    11041 gcagcaaaag caccaaaagt gacaaaagcc tgctcttgcg agaatccaag ctgtttaacg
    11101 aagaaaaccg ccagtacgcc ctgcacgccg tagtagccga atcgctccca taactcgaca
    11161 aaaaagatca tgaaaaatgg gcgaggttgc tgcagcatcc ccatgggtgt tgttgtatta
    11221 atatttatta accttccaat accatcctga aaagtatgaa cgcgtaaggg ataacgcgcg
    11281 aaaaagttta catctgtcag agattcttaa tattgatgcg gtgaatagat taatacacta
    11341 ttacaacaga aaataaccag ctgatcacac taatgatggt gttaaatttt gtattggcag
    11401 gagattttga ttgcgtacgc agatcgtagg ccagataagg ggtttacgct gcatcaggta
    11461 acaaggcgcg ccaaaaagca aaacgcccgc atatgcgggc gttcaggaac acgtcgtgga
    11521 gggatgatta ttcttcttcg tcgcgcagcg gaacaatcag catatcaacg tgaacggtgt
    11581 tgatcagctg acgtgcggaa gacatcagtt tgctccagaa gtcctggtgg tgaccacaaa
    11641 ccaccagatc catatcgtat ttcttgattg catcgaccag aacctggccc aggtcgccgc
    11701 tgccgctcag ggtttcagtg attggatagc ctgcattagt ggaaagctcg gtcagcgcat
    11761 gatgtgtctc ttcagagatg cgtttctgca tatcacccag attcacatca ataagcccgg
    11821 tgtataggtc agagtagttt acatctacgt ggatcagaga aactttcgca ttgtaggggc
    11881 gagccataga gactgctttc tctaccagaa ctttgctttc cggggagaga tcgactgcga
    11941 tgagaatgtg tttataagcc atagtgttac tccttccata aagttgtcga tgactggcca
    12001 gctagcgttt cttgtgccgt tacgtcaccc gcgtcctgcg aactacgtgc gcggggctaa
    12061 cccagccgta aagattcagt gttaagacca tccttacctt atagcgaccc ggatgatccg
    12121 tcaatccgcc ttgcttaccc gttagataaa caaattttcg acatgaatat attgatagtg
    12181 gttaaccttc tggaaaaaaa acaacctgat ctcctacact atctatagag ccgctcggat
    12241 gttggtcacc acaaaagcgg tttctccagg acctggtgtt aactgattgg cagcgtatcg
    12301 ggagcggtag ccccggggtg gaactccgcg ggcaggtcgc cggggaggag gtatgataag
    12361 caccgtcgca ttattttggg ctttatgtgt cgtttgcatt gttaacatgg cgcgctattt
    12421 ctcatcacta cgcgcgttgt tagtggtact gcgtaactgc gatccattgc tctatcaata
    12481 tgttgatgga gggggctttt ttacctcaca tggccaaccc aacaaacagg tgcgtctcgt
    12541 ttggtatatc tatgcccaac gttatcgcga tcatcacgat gatgagttta ttcgccgctg
    12601 tgagcgggtg cgtcggcagt ttattctgac tagcgcattg tgtggtctgg tggtggtcag
    12661 cctgattgca ttgatgattt ggcattaagc acttcagcaa taataacgca taaaaaaagc
    12721 gggccagtaa actgacccgc tcgttttggt gcgtacgatt acaggaactg caaggagaac
    12781 cagtacagcc cgccggaaag tagtaccgca gccggaaggg taaacaccca agccatcaga
    12841 atactggtta cggttttacg ctgtaagccg ccgccatcta ccaccatcgt ccccgcgaca
    12901 gaagaggaga gtacgtgagt ggtggaaacc ggcatcccgg tataactcgc caggccgatt
    12961 gacactgccg ccgtcatctg ggcagacatc ccctgagcgt aggtcatgcc tttcttaccg
    13021 attttctcac cgatagtcgt tgccacacga cgccagccaa tcatcgtacc gatacctaac
    13081 gccagcgcga ccgccatgat gatccacacc ggtgcatact cgatggtgct aagcatgtcg
    13141 gacttcagtt tcttcaacag gcgctgatcg tcagcactca caccaggcat cttcaccact
    13201 ttgtcgatag tgtcagaaac gcacagcata atgcggcgca tctggctacg ttgatcaagc
    13261 gacagcttgt cgtagctttc cacatcggtg gtcaacatac ctttcaggcg gttgagcgcg
    13321 ttaatggtat tcgacggatg gcagtggaac tccgcaggtt gcgttgcgcc agcttccgga
    13381 gccggtacta actgatcagc accggtagcc tgtttgagca gcgcaggatg ctgctcaaag
    13441 taagcttcga cgttgttgat ggcatcacgg gtacgggtga tttcgtagcc agtggcattc
    13501 atgttcacca cgaagcctgc tggcgcgacg ccaatcaata ccaacataac cagaccaatg
    13561 cctttctgac catcgttcgc gccgtgcgaa aacgccacgc cgatagcgga aaggatcagc
    13621 gcgatacgcg tccagaacgg cggctttttc ttgccgtctt tcttttcacg ctccgctggg
    13681 gtcaggtgga tacgggcgcg tttcttggtg ccgctccagt agcgacgcag caagaaaatc
    13741 agaccgccag caaacaccag gccgacaata ggggaaacga tcagagaacc gaaaatactt
    13801 aatactttcg ggatattgag tgcatccacc actgacgtcc cggtcatcaa cgcattggtt
    13861 aaaccaatcc cgatgatcgc gccaatcagc gtatgagagc tggatgcagg taaaccaaag
    13921 taccaggtac ccaggttcca gataatcgcc gccagcaaca tagagaacac catggcaagg
    13981 ccatgagacg atcccatatt aagcagcaga tccgtcggca gcatatgcac aatggcatag
    14041 gcaacactca gaccacccag caaaacaccc aaaaagttga acaccgccgc cataaccacg
    14101 gcgagctgag aacgcatcgc gcgggtatag ataacggttg ccacggcgtt ggctgtgtca
    14161 tggaaaccat tgatggcttc gtagaacagc acaaaagcca gtgcaagcaa taataacagc
    14221 ccggtatgca aatccaggcc agcaaacaaa tgtagcatag gacgttacgc cattttgagg
    14281 acatgaacgc ggcgcattat cagtgacttt cgcggcgcgg gcaaagtgaa atatagactt
    14341 tttttgattt gcctcctgta tggatttcac tcaaaaaata attatcttat ataattcagg
    14401 caaatacttc cttttagtaa tattgatgct ggtgcgacca ctgaggaatc tttacaattc
    14461 acgcccgttt tttctaagag gagcgcaacg tggaaaggtt tgatgccatt attataggcg
    14521 ctggtgcggc gggtatgttc tgttctgcgc tggcaggtca ggcaggacgc cgggttctgc
    14581 tgatcgataa tggtaaaata ctagggcgca aaatccttat gtctggcggt gggcgctgca
    14641 actttaccaa cctttatgtc gaaccaggcg cttatctgag ccagaatccg catttttgta
    14701 agtctgcact cgcacgtttt acccagtggg atttcattga tctggtcaat aaacacggta
    14761 tcgcctggca cgagaaaacg ttagggcaac tcttctgcga tgactccgcg cagcagattg
    14821 tcgacatgct ggtggatgag tgcgagaagg gcaatgtaac cttcagattg cgtagcgaag
    14881 tgctgagtgt ggcgaaggat gaaacaggct tcacgcttga tctgaacggc atgactgtcg
    14941 gttgcgaaaa gctggtcatc gcgaccggtg ggctgtcaat gccggtgctg ggcgcatcgc
    15001 cgtttggtta taagattgcc gaacaatttg gcctcaacgt gctgccgacc cgcgcgggtc
    15061 tggtgccatt cactctgcat aaaccgttgc tcgaagagtt acaggtgctg gcgggcgtgg
    15121 cggtgccttc cgtgattacc actgaaaacg gcaccgtttt ccgtgagaac ttactcttca
    15181 cccaccgcgg cttatctgga ccggcggtgt tgcagatttc aagctactgg caaccgggtg
    15241 aatttgtcag tatcaatctg ctaccggatg tggacctcga aaccttcctg aatgagcagc
    15301 gtaacgcaca tccgaatcaa agcctgaaaa acacactggc ggttcatcta ccgaagcggt
    15361 tggttgaacg cttacagcaa ctcgggcaaa tcccggatgt ttcgctaaaa cagctcaacg
    15421 tgtgtgacca acaggcactg attagcacat tgaccgactg gcgcgtacaa cccaacggca
    15481 ctgaaggcta tcgcactgcc gaagtgacgc tcggcggcgt ggacaccaac gaactttctt
    15541 cacggacgat ggaagcgcgc aaagtgcctg ggctgtactt catcggcgaa gtgatggacg
    15601 ttaccggctg gctggggggc tataacttcc agtgggcgtg gtcgagtgca tgggcttgtg
    15661 cgcaggattt ggcgtcaatt gtgaaataac catgacgttt tcccgtcagc cgtttgtata
    15721 tgtcgttatt aagtcatttt ggtacggttc tgcatccgac attctggtgt taactcgctg
    15781 ttttgttagc gaattaacgc tattgccgtt gtggttattt tatagtgtat ttattctcag
    15841 agaatatata ttactgaaat aaaaaacaca aagaataacc aaaggaaatt atttgaaacg
    15901 taaaaggtat atatttttaa tctgtaaaat ttaaaatcac cccctctgga ttgaatcaac
    15961 ttgctctatt taagacgatt gtttttacat tttcaccacc tgtatcaggt ggtgtttttt
    16021 tattggcggc aatgccttta tcttattttt attacgttgt aaatatggct attcctaaat
    16081 gaaatttgat ttgctcagtt tatttgtgat gtcaggggag tgcttgccat gagagtcgtg
    16141 atattattat tcatcatttt tataccgagt gtggcggatg ctttttcatt cgatagttca
    16201 ataattaatc tcaaatgtcc
//
LOCUS       DADMGC010000089        16148 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.089, whole genome shotgun sequence.
ACCESSION   DADMGC010000089 DADMGC010000000
VERSION     DADMGC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16148
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.089"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(188..520)
                     /gene="emrE"
                     /locus_tag="JNZ73_002782"
     CDS             complement(188..520)
                     /gene="emrE"
                     /locus_tag="JNZ73_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HAY8733009.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(865..1179)
                     /gene="fliE"
                     /locus_tag="JNZ73_002783"
     CDS             complement(865..1179)
                     /gene="fliE"
                     /locus_tag="JNZ73_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAY8733010.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAAIDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            1393..3051
                     /gene="fliF"
                     /locus_tag="JNZ73_002784"
                     /pseudo
     CDS             1393..3051
                     /gene="fliF"
                     /locus_tag="JNZ73_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
     gene            3044..4039
                     /gene="fliG"
                     /locus_tag="JNZ73_002785"
     CDS             3044..4039
                     /gene="fliG"
                     /locus_tag="JNZ73_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAY8733011.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4032..4718
                     /gene="fliH"
                     /locus_tag="JNZ73_002786"
     CDS             4032..4718
                     /gene="fliH"
                     /locus_tag="JNZ73_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAY8733012.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAALGVV"
     gene            4718..6091
                     /gene="fliI"
                     /locus_tag="JNZ73_002787"
     CDS             4718..6091
                     /gene="fliI"
                     /locus_tag="JNZ73_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HAY8733013.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRSQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6110..6553
                     /gene="fliJ"
                     /locus_tag="JNZ73_002788"
     CDS             6110..6553
                     /gene="fliJ"
                     /locus_tag="JNZ73_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HAY8733014.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            6550..7677
                     /gene="fliK"
                     /locus_tag="JNZ73_002789"
     CDS             6550..7677
                     /gene="fliK"
                     /locus_tag="JNZ73_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HAY8733015.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSASLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGDDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7782..8246
                     /gene="fliL"
                     /locus_tag="JNZ73_002790"
     CDS             7782..8246
                     /gene="fliL"
                     /locus_tag="JNZ73_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HAY8733016.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8251..9255
                     /gene="fliM"
                     /locus_tag="JNZ73_002791"
     CDS             8251..9255
                     /gene="fliM"
                     /locus_tag="JNZ73_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HAY8733017.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDILPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9252..9665
                     /gene="fliN"
                     /locus_tag="JNZ73_002792"
     CDS             9252..9665
                     /gene="fliN"
                     /locus_tag="JNZ73_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HAY8733018.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILNN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9668..10033
                     /gene="fliO"
                     /locus_tag="JNZ73_002793"
     CDS             9668..10033
                     /gene="fliO"
                     /locus_tag="JNZ73_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HAY8733019.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            10033..10770
                     /gene="fliP"
                     /locus_tag="JNZ73_002794"
     CDS             10033..10770
                     /gene="fliP"
                     /locus_tag="JNZ73_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HAY8733020.1"
                     /translation="MRRLFSVAPVLLWLIMPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10780..11049
                     /gene="fliQ"
                     /locus_tag="JNZ73_002795"
     CDS             10780..11049
                     /gene="fliQ"
                     /locus_tag="JNZ73_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HAY8733021.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11058..11762
                     /locus_tag="JNZ73_002796"
     CDS             11058..11762
                     /locus_tag="JNZ73_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HAY8733022.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFNGHLWLISLLVDTFHTLPIGSEPLNSNAFLAPTKAGSLIFLNGLMLA
                     LPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFCEHLFS
                     EIFNLLADIISELPLI"
     gene            12047..12670
                     /gene="rcsA"
                     /locus_tag="JNZ73_002797"
     CDS             12047..12670
                     /gene="rcsA"
                     /locus_tag="JNZ73_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HAY8733023.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12714..12956)
                     /gene="dsrB"
                     /locus_tag="JNZ73_002798"
     CDS             complement(12714..12956)
                     /gene="dsrB"
                     /locus_tag="JNZ73_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HAY8733024.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDSGPRRPGVVLAVEE
                     FSEGTMYLVALEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            13065..13292
                     /gene="yodD"
                     /locus_tag="JNZ73_002799"
     CDS             13065..13292
                     /gene="yodD"
                     /locus_tag="JNZ73_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HAY8733025.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13590..14405
                     /locus_tag="JNZ73_002800"
                     /pseudo
     CDS             13590..14405
                     /locus_tag="JNZ73_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(14402..16096)
                     /gene="yedQ"
                     /locus_tag="JNZ73_002801"
     CDS             complement(14402..16096)
                     /gene="yedQ"
                     /locus_tag="JNZ73_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HAY8733026.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNFLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFMRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLIFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLARVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDHA"
BASE COUNT         4022 a         4155 c         4236 g         3735 t
ORIGIN      
        1 aaacgataag ttttttcata aaaacctcat tgttgtgacc tatcgttatt ttatttgatg
       61 tgccttgatc tcattatgca taaaggcaca atgatcgcgc aaatgcaatc ataattataa
      121 aggtgccccg aacattagcc ataatatgca caaatcagat tcctgttatc tcaaggagaa
      181 ttatttttta atgcggtgca cttcgtgaca acaagttaat gactaacaca ccggcacaaa
      241 tcaacatcat gcctatgata gctggcaggt ccagtcgttg accaaaaaat ccccaggaaa
      301 gcaaactaat cagaacgata ccgactcctg accagatagc ataagcaatc cctgtaggta
      361 tataagccag cgtctgagct aataaccaga atgatgcaca atagcaaata attgtgccaa
      421 cagagggcca taaccgtgta aacccctctg aaaacttcat taaggttgta ccaatgacct
      481 ctgcaagtat tgcgccacca agataaatat aagggttcat agcatattct ttcctgttca
      541 tactggagag aattgtacta cagtttgaac acaactcacc gttttcatcc tagtgtatgt
      601 gtatccaagg ggctctggcg tggcaagaga taaaggcagg aacaataatg atcagaagga
      661 accccaggct aacgaatgag ttcactcttc ctgggagcaa agcgaagaca aaacatgtta
      721 tcgcgtttac ggatcgtgga tggcagaaaa caaccaggat caggtatcca ttttaaacca
      781 gaaattgagt gagtttgccc catccatgcc ccacgctgtg ggatcggata taattaaaca
      841 agcatagtca acaggttaca caacctacac ctgcatagac atcacttcct gatacgccgc
      901 caccagctta ttacgcacct gaatccccat ttgcatagaa actgaggctt tttgcatatc
      961 ggtcatcaca tcgtttagcg ccacgccggg ttcaccgagg gtgaattttt ctgcctgcgt
     1021 gcgggcgact gtttgtgtat cgcttatgcg atcgatcgcg gcgtgcagct gcccggcaaa
     1081 actaatggtc ggttgcggca gtgattcctg cgcacgcgca ctcatcgccg tagcctgtaa
     1141 ctggctgata accccttcaa tcccctgtat cgctgacatt ttcgtctccc ggataatttc
     1201 tggtaacaaa gcctaccagt aagtcaataa gacaaaggcg ctaaatagca acaaaaaaac
     1261 gggtttattg gcggatagaa aaaacgaaag cacaaataat gggagcgtca atttttcgag
     1321 tttgctgacc cgagagtgag tcttgttcca ctttgccaat aacgccgtcc ataatcagcc
     1381 acgaggtgcg cgatgaatgc gactgcagcc cagacaaaat ctcttgagtg gcttaatcgc
     1441 ctgcgtgcga atccgaaaat tccattgatt gttaccggtt ccgcggcagt ggcggtcatg
     1501 gtcgcactga tcctgtgggc gaaagccccc gactaccgca cattattcag caatctttcc
     1561 gatcaggatg gtggcgcaat tgtcagccaa ctgacgcaaa tgaatattcc ttaccgcttc
     1621 agcgaagcca gcggcgctat tgaggttccg gcagataaag ttcacgaact gcgcctgcgc
     1681 ctggcgcaac aaggtttgcc caaaggcggc gcggtcggtt tcgaactgct cgatcaggaa
     1741 aagtttggta tcagccagtt cagcgaacag gtgaattatc agcgggcgct ggaaggcgag
     1801 ctttctcgta ccatcgaaac tatcggcccg gtaaaagggg cgcgcgtaca tctggcaatg
     1861 ccgaaaccgt ctttattcgt ccgtgaacaa aaatcccctt ctgcatcggt gacggtaaat
     1921 ctgttaccca gccgcgcact cgatgaaggg caaattagcg ctattgtaca tctggtttcc
     1981 agcgcggttg ctggtctgcc gcctggaaac gtcacgctgg tggatcaggg cgggcatctg
     2041 ttaacccagt ccaataccag cgggcgcgat cttaatgacg ctcagttgaa atatgccagc
     2101 gatgtcgaag gccgtattca gcggcgtatt gaagcgatcc tgtcgcctat tgttggtaac
     2161 ggtaatattc acgcctaggt cacggcgcaa ctggacttcg ccagtaaaga acaaacggaa
     2221 gaacagtatc gccctaacgg tgatgaatct catgcggcgc ttcgttcacg ccagcttaat
     2281 gagagcgagc aaagcggttc cggttatccg ggcggcgtac cgggggcgtt gtcgaatcaa
     2341 ccggcacctg cgaataacgc gccaatcagc acgcctccga caaatcaaaa taaccgccag
     2401 cagcaggcga gcaccaccag caatagtggg ccgcgtagca cacagcggaa tgaaaccagt
     2461 aactacgaag tcgatcgcac cattcgtcat accaaaatga acgtgggcga tgtgcaacgt
     2521 ctgtcagtcg cggtagtggt gaattacaaa accttgccag acggtaaacc attgcctctc
     2581 agcaacgaac agatgaagca aattgaagct ctgacccgcg aggcgatggg cttttctgaa
     2641 aaacgcggtg attcgctcaa tgttgttaac tcgccgttca atagcagtga cgaaagcggc
     2701 ggagcactgc cgttttggca acagcaagtg tttatcgatc agttgctggc tgccggtcgc
     2761 tggttgctgg tactgctggt ggcgtggttg ctgtggcgga aagcggtacg cccgcagcta
     2821 acacgtcgcg ccgaggcggt gaagactgtg cagcaacagg cgcaggcccg cgaggaagtg
     2881 gaagatgcgg tggaagtccg tctgagcaaa gacgaacaac tccaacaacg gcgcgctaac
     2941 caacgactgg gggcagaagt catgagccag cgtatccgtg aaatgtctga taacgatccg
     3001 cgcgtggtgg cgctggtcat tcgccagtgg ataaataacg atcatgagta acctgacagg
     3061 caccgataaa agcgtcatcc tgctgatgac cattggcgaa gaccgggcgg cagaggtgtt
     3121 caagcacctc tcccagcgcg aagtgcaaac cctgagcgct gcaatggcga acgtcacgca
     3181 gatctccaac aagcagctaa ccgatgtgct ggcggagttt gagcaagaag ctgaacagtt
     3241 tgctgcactg aatatcaacg ccaacgatta tctgcgttcg gtattggtca aagctctggg
     3301 tgaagaacgt gccgccagcc tgctggaaga tattctcgaa acccgcgata ccgccagcgg
     3361 tattgaaacg ctcaacttta tggagccgca gagcgccgcc gatctgattc gcgatgagca
     3421 tccgcaaatt atcgccacca ttctggtgca tctgaagcgc gcccaggccg ccgatattct
     3481 ggcgttgttc gatgaacgtc tgcgccacga cgtgatgttg cgtatcgcca cctttggcgg
     3541 cgtgcagcca gccgcgctgg ccgaactgac cgaagtactg aatggcttgc tcgacggtca
     3601 gaatctcaag cgcagcaaaa tgggcggcgt gagaacggca gccgaaatta tcaacctgat
     3661 gaaaacgcag caggaagaag ccgttattac cgccgtgcgt gaattcgacg gcgagctggc
     3721 gcagaaaatc atcgacgaga tgttcctgtt cgagaatctg gtggatgtcg atgatcgcag
     3781 cattcagcgt ctgttgcagg aagtggattc cgaatcgctg ctgatcgcgc tgaaaggggc
     3841 cgagcagcca ctgcgcgaga aattcctgcg caatatgtcg cagcgtgccg ccgatattct
     3901 gcgcgacgat ctcgccaacc gtggtccggt gcgtctgtcg caggtcgaaa acgaacagaa
     3961 agcgattctg ctgattgtgc gccgtcttgc cgaaactggc gagatggtga ttggcagcgg
     4021 cgaggatacc tatgtctgat aatctgccgt ggaaaacctg gacgccggac gatctcgcgc
     4081 caccaccggc agagtttgtg ccaatggtcg agtcggaaga aaccattatt gaagaggccg
     4141 aacccagcct tgagcagcaa ctggcgcaac tgcaaatgca ggcccacgag cagggttatc
     4201 aggcggggat tgccgaaggt cgccagcaag gtcacgaaca gggctatcag gaaggactgg
     4261 cccaggggct ggaacaaggt ctggcagagg cgaaggctca acaagcgcca attcatgccc
     4321 ggatgcagca actggtcagc gaatttcaaa ctacccttga tgcgcttgat agtgtgatag
     4381 cttcgcgcct gatgcagatg gcgctggagg cagcacgtca ggtgatcggt cagacaccaa
     4441 cggtggataa ctcggcactg atcaaacaga tccaacagtt gttgcagcaa gaaccgttat
     4501 tcagcgggaa accacagctg cgcgtacacc cggatgatct gcaacgtgtg gatgatatgc
     4561 tcggcgcaac tttaagtttg catggctggc gcttgcgggg cgatcccacc ctccaccctg
     4621 gcggatgtaa agtctccgcc gatgaaggcg atctcgacgc cagtgtcgcc actcgctggc
     4681 aagaactctg ccgtctggca gcactaggag tggtgtaatg accacgcgcc tgactcgatg
     4741 gctaaccacg ctggataact ttgaagcaaa aatggcgcag ttgcctgcgg tacgtcgcta
     4801 cgggcgatta acccgcgcta ccgggctggt gctggaagcc accggattac aattgccgct
     4861 cggcgcaacc tgtgtcattg agcgccagaa cggcagcgaa acgcacgaag tagaaagcga
     4921 agtcgttggc tttaacggtc aacggctgtt tttaatgccg ctggaggaag tggaaggtgt
     4981 cctgcccggc gcgcgtgtat atgccaaaaa catttcggca gaagggctgc aaagcggcaa
     5041 gcagttgccg ctcggtccgg cgttattagg tcgtgttctg gacggtagcg gtaaaccgct
     5101 cgatggcctg cccttccccg atacgacgga aaccggtgcg ctgattaccc cgccatttaa
     5161 cccgttgcaa cgtacaccga ttgaacatgt gctggacacc ggcgtgcgcc caatcaatgc
     5221 cctgcttacc gtcggacgcg ggcagcgtat ggggctgttt gccgggtcgg gcgttggtaa
     5281 aagtgtgctg ctggggatga tggcacgtta cacccgcgcc gatgtcattg tcgtgggttt
     5341 gattggtgaa cgtgggcgcg aagtaaaaga ttttattgag aatatcctcg gtgccgaagg
     5401 acgtgcacgc tcagtggtga ttgccgctcc ggcggatgtt tctccgctcc tgcgaatgca
     5461 aggtgccgcc tatgccacgc gcattgccga agattttcgc gatcgtagtc agcatgtgtt
     5521 gctgattatg gactccctca cccgctacgc gatggcccag cgtgaaattg cgctggcgat
     5581 tggcgaacca cctgccacta aaggttatcc accgtcggtg tttgccaaat taccggcact
     5641 ggtcgagcgt gccggtaatg gcattagcgg cggcggctcg attaccgcgt tttataccgt
     5701 gctcaccgaa ggcgatgacc agcaggaccc cattgctgac tccgcgcggg caatcctcga
     5761 cggtcacatt gtgctgtctc gccgactggc ggaagccggg cactatccgg ctatcgatat
     5821 tgaagcgtcg atcagtcgcg caatgacggc gttgatcagt gagcaacatt acgcgcgagt
     5881 gcgcaccttc aaacagctgt tgtcgagttt tcagcgtaac cgcgatctgg ttagcgtcgg
     5941 cgcgtatgcc aaaggcagcg atccgatgct cgataaagcc atcgccctgt ggccgcagct
     6001 ggagggctat ttgcaacaag gcatttttga acgcgcggac tgggaagcgt ctctccaggg
     6061 gctggagcgt attttcccga cagtgtcata acccaggaga taacggcaga tggcggaaca
     6121 tggtgcgctg gcgaccctga aagatctggc agaaaaagag gtagaggatg ccgctcgcct
     6181 gctgggtgaa atgcgtcgcg gatgtcagca ggcggaagaa cagctcaaaa tgctgatcga
     6241 ttatcagaat gaatatcgca ataacctcaa tagcgatatg agtgccggga tgaccagcaa
     6301 ccgctggatc aactatcagc agtttatcca gacgctggaa aaagccatta ctcagcatcg
     6361 ccagcaactt aatcagtgga cgcagaaagt tgacattgcc ctgaacagct ggcgagaaaa
     6421 gaaacaacgt ttgcaggcct ggcagacact gcaggaacgg caatccacgg cggcactgct
     6481 tgcagaaaac cgcctcgatc agaaaaagat ggatgagttc gcccagcgcg ccgccatgag
     6541 gaaacctgaa tgattcgctt agcgcccttg attaccgccg acgttgacac caccacattg
     6601 cctggcggca aagccagcga tgctgcacaa gattttctaa cgttgttgag cgaagcatta
     6661 gcaggcgaga caacaactga caaagcggcc ccccagttgc tggtggcaac agataagccc
     6721 acgacaaaag gcgagccgct ggtcagcgat attcttgccg atgcgcaaca agcggattta
     6781 ctgatccctg tggatgaaac acttcctgtc atcaacgacg aacaatccac atcaacaccg
     6841 ttaaccaccg ctcagacaat gacgttggct gcggtggctg acaaaaatac gacaaaagac
     6901 gaaaaagcgg atgatcttaa tgaagacgtc accgcaagtc tgagcgccct ttttgcgatg
     6961 ttgccgggtt ttgacaatac gcccaaagtg actgatgcac cgtcaaccgt gttaccggca
     7021 gagaaaccaa cgctcttcac aaaactgact tctgcgcaac tcactacagc acagcctgat
     7081 gacgcccccg gcacaccagc tcagccatta acaccgctgg tagcagaagc ccagagtaaa
     7141 gcggaagtca tcagcacgcc ttcgccggtg accgctgacg ccagcccgct aatcactcca
     7201 caccagacac agccactgcc caccgtcgcc gcgcctgttt tgagtgcatc gctgggttct
     7261 cacgaatggc aacaatcatt gagccagcat atttcgctgt tcacccgcca ggggcaacaa
     7321 agtgcagagt tgcgtctgca cccacaggat ttaggtgaag tgcaaatttc cctcaaagtg
     7381 gatgataacc aggcgcagat ccagatgatt tcaccgcatc agcacgtacg cgccgccctg
     7441 gaagcagcgc tgccggtact gcgtacgcag ctggccgaaa gtggcattca gttagggcaa
     7501 agcaacatta gtggcgaaag ctttagtggt cagcagcagg ccgcttccca gcaacagcaa
     7561 agccaacgca cagcaaacca tgaacctctg gcgggggatg acgacgatac gcttccggtt
     7621 cccgtctctt tacaagggcg tgtaacaggt aacagcggcg ttgatatttt cgcctaacgt
     7681 cagaggtagc accgtaatcc gcgtcttttc cccgctttgt tgcgctcaag acgcaggata
     7741 attagccgat aagcagtagc gacacaggaa gaccgcaaca catgactgat tacgcgataa
     7801 gcaagaaaag caagcgatcg ctttggatcc cgattctggt attcattacc ctcgcggcct
     7861 gtgccagcgc aggttacagc tactggcatt cgcatcaggt tgccgctgac gacaaagcgc
     7921 agcaacgcgt cgtgccctcc ccggtcttct atgcgctgga tacctttacg gtcaatttgg
     7981 gcgatgcgga tcgcgtgctt tatatcggca taaccctgcg actgaaagat gaagcaaccc
     8041 gctcgcggct gagtgagtat ttgccggaag tccgtagtcg cttgctgtta ctgttttcac
     8101 gtcaggatgc tgccgtactg gcgacagaag aaggcaagaa aaacctgatt gccgagatta
     8161 aaaccacact ttccaccccg cttgttgccg ggcaaccgaa acaggatgtc accgacgtgc
     8221 tgtataccgc ttttattctg cgataacgac atgggcgata gtattctttc tcaagctgaa
     8281 attgatgcgc tgttgaatgg tgacagcgaa gtcaaagacg aaccgacagc cagtgttagc
     8341 ggcgaaagtg acattcgtcc gtacgatccg aatacccaac gacgggttgt gcgcgaacgt
     8401 ttgcaggcgc tggaaatcat taatgagcgc tttgcccgcc attttcgtat ggggctgttc
     8461 aacctgctgc gccgtagccc ggacattacc gtcggggcca tccgcattca gccataccat
     8521 gaatttgccc gcaacctgcc ggtgccgacc aacctgaacc taatccacct gaaaccgcta
     8581 cgcggcacag ggctggtagt gttctcgccg agtctggtgt ttatcgccgt ggataacctg
     8641 tttggcggcg atggacgctt cccgaccaaa gtggaaggtc gcgagtttac ccatactgaa
     8701 cagcgcgtca tcaaccgtat gttgaagctg gcgctggaag gctatagcga cgcctggaaa
     8761 gcgattaatc cgctggaagt tgagtacgtg cgttcggaaa tgcaggtgga atttaccaat
     8821 atcaccacct cgccgaacga cattgtggtt aacacgccgt tccatgtgga gattggcaac
     8881 ctgaccggcg aatttaatat ctgcctgcca ttcagcatga tcgagccgct acgggaattg
     8941 ttggttaacc cgccgctgga aaactcgcgt aatgaagatc agaactggcg cgataacctg
     9001 gtgcgccagg tgcagcattc acagctggaa ctggtggcca actttgccga tatctcgcta
     9061 cgcctgtcgc agattttaaa actgaaaccc ggcgacatcc tgccgataga aaaacccgat
     9121 cgcatcatcg cccatgttga cggcgtcccg gttctgacca gtcagtacgg cactctcaac
     9181 ggtcagtatg cgttacggat agaacatttg attaacccga ttttaaattc tctgaacgag
     9241 gaacagccca aatgagtgac atgaataatc cggccgatga caacaacggc gcaatggacg
     9301 atctgtgggc tgaagcgttg agcgagcaaa aatcaaccag cgaaaaaagc gctgccgacg
     9361 cggtgttcca gcaatttggc ggtggtgatg tcagcggaac gttgcaggat atcgacctga
     9421 ttatggatat tccggtcaag ctgaccgtcg agctgggccg tacgcggatg accatcaaag
     9481 agctgttgcg tctgacgcaa gggtcagtcg tggcgctgga cggtctggcg ggcgaaccgc
     9541 tggatattct gaacaacggt tatttaatcg cccaaggcga agtggtggtt gttgccgata
     9601 aatatggcgt gcggatcacc gatatcatta ccccgtccga gcggatgcgc cgcctgagcc
     9661 gttagtgatg aataaccatg ctactgtgca atcttccgcg ccggtttctg ctgagccact
     9721 gctgcaggtg agcggcgcac tcatcgccat tattgccctg atcctcgctg ctgcctggct
     9781 ggtaaaacgg ctgggatttg cccctaaacg cactggcgtt aacggtctga aaattagcgc
     9841 cagtgcttcg ctgggcgcgc gtgaacgggt cgtggtggtc gatgtggaag atgcacggct
     9901 ggtgctcggc gttaccgcag ggcaaatcaa tctgctgcat aaacttcccc cttcagcacc
     9961 aacggaagag ataccgcaga ccgattttca gtcggtcatg aaaaatttgc ttaagcgtgg
    10021 cgggagatcc tgatgcgtcg tttgttttct gtcgcaccgg tccttctctg gctgattatg
    10081 cccctcgcct tcgcgcaact gccgggtatc accagccagc cgctacctgg cggtggacaa
    10141 agctggtcgc tcccggtgca gacgctggtg ttcatcacct cgttgacgtt cattccagcg
    10201 attttactga tgatgaccag tttcacccgc atcattattg tttttggttt attgcgtaac
    10261 gcgctgggaa cgccctccgc gccacctaac caggtattgc tggggctggc actgtttttg
    10321 acctttttta ttatgtcgcc agtgatcgac aaaatttatg tcgatgcgta ccagccattc
    10381 agcgaagaga aaatctcaat gcaggaggcg ctggaaaaag gggcgcagcc gctgcgtgag
    10441 tttatgctgc gtcagacccg cgaggcagat ttagggttgt ttgccagact ggcgaatacc
    10501 ggcccgttgc aggggcctga agctgtaccg atgcgcattt tgctcccggc ctacgtgacc
    10561 agcgagttga aaaccgcatt tcagataggc ttcacgattt tcatcccttt tttgattatc
    10621 gacctggtga ttgccagcgt gttgatggca ttggggatga tgatggttcc ccccgccacc
    10681 attgctctgc cctttaaact gatgctgttt gtactggtgg atggctggca attgctggtc
    10741 ggttcgctgg cgcagagctt ttacagctag agaggcaaaa tgacacctga atcggtcatg
    10801 atgatgggaa ctgaagcgat gaaagtcgcg ctggcactgg cagccccgct gttgctggta
    10861 gcgctggtca cgggccttat catcagtatt ttgcaggcag ccacgcagat taacgaaatg
    10921 acgctgtcgt ttattccgaa aatcatcgcc gtatttatcg ccatcattat tgccggaccg
    10981 tggatgctca atctgttgct ggattacgtc cgcaccttgt tcactaacct gccgtatatc
    11041 atcgggtagc ccgtacaatg atgcaggaga caagcgatca atggctttcc tggttaagcc
    11101 tgtacttctg gccgttactg cgcgtgctgg cgctcatttc taccgcgcca attctgagcg
    11161 aacgcagcgt gccgaaacgg gtaaaactgg gtctggcaat gatgatcacg ttcgccattg
    11221 ccccatcatt acctgccaac gatgttcctg ttttttcgtt ctttgctctg tggctggccg
    11281 tgcagcagat cctgatcggc attgcgcttg gttttaccat gcaatttgcc tttgccgctg
    11341 tgcgaaccgc tggcgaaatt atcggtctgc aaatggggct gtcatttgcg acatttaacg
    11401 gtcatttatg gttgatttca ctgctggtcg atacctttca caccctgccg attggtagcg
    11461 aaccgttgaa cagcaatgcg tttctggcac ccaccaaagc agggagtttg attttcctta
    11521 acgggctgat gctggcgtta ccgctcatta ctctgttgct gaccctgaat ctggcattag
    11581 gtttactgaa tcgtatggcc ccgcaattat ccatttttgt tattggattt ccattaactc
    11641 tgactgtcgg catctcttta atggcggcat taatgccgtt aattgcgcct ttttgcgaac
    11701 atttattcag tgaaattttt aatttgctgg ctgatattat tagtgaatta ccattaatat
    11761 aattccgtaa cgtttatcat gctaccctaa ggattatccg aaaaataata tctgcgaaca
    11821 tcttccagga tactcctgta gcgaaatatt tgttttaagc tcactcacat atcgcaacat
    11881 ttactttaag acaattccag gcaaattata caacacttta cgggatagta agtccgcctg
    11941 aaaaattgcg agagtggcgc attaggtgac ccatgttgtt ccgtttagtc atgatgaaat
    12001 attcaggtaa ggggaattat cgttacgcat tgagtgaggg tatgccatgt caacgattat
    12061 tatggattta tgtagttaca cccgactagg tttaaccggg tatctgttga gtagaggggt
    12121 taaaaaaaga gaaatcaacg acattgaaac cgttgatgac cttgccatag cttgtgattc
    12181 acagcgtcct tcagtggtgt ttattaatga ggactgtttc atccacgatg cttctaacag
    12241 tcagcgcatc aagcacatca ttaatcaaca tcccaatacg ttatttatcg tttttatggc
    12301 aattgccaat gttcattttg atgaatatct attggtcaga aaaaatttat tgatcagttc
    12361 taaatcgatt aaaccggaat ctctcgacga tatccttggc gatattctga aaaaagagac
    12421 aacgataacc tcgtttttaa atatgccgac gttatcattg agccgaaccg aatcgagtat
    12481 gttgcgaatg tggatggcag gtcagggaac cattcaaatc tccgaccaaa tgaatatcaa
    12541 agccaagacc gtttcatcgc ataaaggtaa tattaaacgt aagatcaaaa cgcataataa
    12601 acaggttatc taccatgtcg tccgactgac ggataatgtg actaatggta tttttgtcaa
    12661 catgcgctaa cacattctga ctggtggttt cccaccagtc aggctgaata agattactct
    12721 gctttctcaa caaagatacc gtcctgatgc cctgcttcat taaagaacca gatgccgagc
    12781 gggtagtctt ccagcgcaac caggtacatt gtgccttcac taaactcctc aactgccagt
    12841 accactccag gacgacgcgg accgctatcc gttttgactg ttacccgatc attcaccttc
    12901 atcgttttcc tcctgtggct ttgtgccagt gtagaacaat ttcgtttttc tggcagcgcc
    12961 aagcgcgcgc gagtgctgat tttctcgacg gtctatactt aagagatgcc aggcggactt
    13021 aacgactggc ggcaacaaca gagtaacggt tgcgaggaaa gatgatgaaa accgcaaaag
    13081 tgtacagcga taccgcaaaa cgtgaggtca gcgtcgatgt cgatgccctg ctggcggcga
    13141 tcaatgaaat tagcgaaagc gaagttcatc gcagccagaa cgattctgaa cacgttagcg
    13201 tcgatggacg tgaatatcat acatggcgtg aattggcgga tgccttcgaa ctggatattc
    13261 atgacttcag cgtctctgaa gtgaatcgtt gaatgcacaa taaaaaaatc ccgaccctga
    13321 gggtgtcggg atgaaacttg cttaagcaag aagcacttaa aaaattcgtt acaccaggaa
    13381 atctgatgtg ttcatcacct tatctgcaat ttttttcgct gacaagaaaa tattcgcgct
    13441 atgaatgatt aacgctactt atgaatagaa atgtgacgcg cgtcacccct tggtggctct
    13501 ggtgtcaacg ggatcgctcc cgaaaaaatc atctttgatt atttgtgctg ttttgagaat
    13561 catcctggta aggggagtac tgcgcgacca tgttttcaat tcaacaacca ctactggttt
    13621 ttagcgatct tgatggcacc ctgctggaca gtcatagtta tgactggcaa ccggcagccc
    13681 cctggctcag ccgtttacac gaagccaata ttcccgtcat tctctgtagc agtaaaactt
    13741 cagcggaaat gctgtacttg caaaaaatgt tggggctaca aggcttaccg ctgattgcag
    13801 agaatggcgc agtgatccag cttgctgaac aatggcagga tatagacggt tttccacgca
    13861 tcatctcagg tattagccat ggcgaaatct gccaggtttt aaatacgcta cgcgagaaag
    13921 aacattttaa attcacgact tttgatgatg tcgacgatgc aaccattgcc gaatggacgg
    13981 gattaagccg tagctaggcg gcgctgacgc agctgcatga ggcgtcggta acgctaatct
    14041 ggcgcgacag tgacgagcgt atggcacaat ttatcgctcg tctgaacgaa ctgggcttgc
    14101 agtttatgca gggtgcgcgc ttctggcacg tactggatgc ctctgccgga aaagatcagg
    14161 ctgccaactg gattatcgcg acctatcaac aattgtcagg cagacgccca accacacttg
    14221 gcctgggcga tgggccaaac gatgcgcctt tactggaggt aatggattac gcggtgattg
    14281 tgaaagggct aaaccgtgaa ggggtacatc tgcatgatga ggatccggcc cgcgtctggc
    14341 gaacgcagcg tgaaggaccg gaaggctggc gtgaagggct ggaccatttt ttctccgccc
    14401 gttaagcgtg atcgctcgcg aatacccgat tgcgcccggc ctgttttgcg aggtaaagcc
    14461 gacggtcggc caatgactgg agttgttcaa aatcataatc accggtttcc tcgctgctac
    14521 ttacccccag cgaggcactg atgcgtatcg ttgtactctt ggcgatcagc atctcttttt
    14581 catttaactt caggcgaata cgttctgcga cctccgcagc ctgcgtcaga ttcgcgcctg
    14641 gcagaatcac acaaaactcc tcaccaccga cccgcccggc aacgtcctgc gcacgcaagg
    14701 aactgctaat taatccggca gcatgagaaa gaacacggtc gcccgcctga tgaccaaagc
    14761 ggtcattaat cgctttaaaa tggtcaagat cgacctgaat gacagaaaaa ggatgttggt
    14821 gcgtctgaca caatttagcg agcggacggg ctttttcgaa cagtgcgcca cggttatata
    14881 aacgcgttaa ggtgtcgtgc cacgcctgcc actgcaacga gctttgcaga acatacatgt
    14941 tgctgaccat ccggcgaatc acataccagg aaaggagtaa catggtggta aagagcgccc
    15001 acagcagggt taatgcaatg ctgatactgc cgaaatcgcc gcgcacgcct tcgcttagcg
    15061 tatggacgcg cgccagcaca ccgtcgaaat ggtccagacg ttcccagcta acatagcgac
    15121 tgtccatacg aatgccgccc cgcgtgtcat gttccattgc ctgcgccagc aaggccagtt
    15181 cacgaggatc aaaaatattc cctgttggat ggtcaggatt ggaagaggtc aaaaatatca
    15241 gcttactgtc atagagctga tactcaccat cgaggttttt atcgatggcg tttcttaaaa
    15301 attgctgcat agtacgcacg ggaatactca tccccagcac gccataccag tagttattac
    15361 tgtctaccgg aacactgacg gtaacctgcg gttcagtatt gctggcgtgt tccggttgcg
    15421 aagtgaacca gcgtaccgcg cggtgacgat tttctcgttg cgaatggccg ataaaccagg
    15481 gttgggtgac atagccgtaa taacgcgttg gtacattgcg cataaacaag gtcggctgcg
    15541 tcgaaacgta aaatccagca cgcgagacat acatcgcctg ttcaaccatc gacgaggagt
    15601 tgtgcgccag tcgcagcaag taaccaactt ccagtgcagc ggtaatttca ttgtcgaggc
    15661 tttcattttc gcgagacagg aaattcccct cgctgactaa tgcatccgat acgccattga
    15721 ctggcagggt gcgccgtcgg ttgagttcga tttgccaggc gtgctcgtcg cgatgctgtt
    15781 cgaactcggt tacggcatta cgcagagagg tgaaatccag tggcgccacg agagcttcgc
    15841 gcatgccatt acgcaaaaag atcagtttgt cgacgttata ctgcaaatgc ttatcgagcg
    15901 cgttggctac gttttccaga tgattacgct ggctggagat ataggcatct tccagcacca
    15961 ccacttcacg ccaggtgagc aaggtggaaa aaagcaatac cacgataaag cagagattaa
    16021 cgacatgacc gggccccagg cggcgtgcga gttttttcaa ccagctctgg ttttccattt
    16081 ttgtctcgtg ctgcaccctg acgactccca acctgctttt ttatgattct ggcataaccc
    16141 ggcaatag
//
LOCUS       DADMGC010000090        16080 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.090, whole genome shotgun sequence.
ACCESSION   DADMGC010000090 DADMGC010000000
VERSION     DADMGC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.090"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(283..987)
                     /locus_tag="JNZ73_002802"
     CDS             complement(283..987)
                     /locus_tag="JNZ73_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY8733027.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGLGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1124..1576)
                     /gene="moaE"
                     /locus_tag="JNZ73_002803"
     CDS             complement(1124..1576)
                     /gene="moaE"
                     /locus_tag="JNZ73_002803"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HAY8733028.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(1578..1823)
                     /gene="moaD"
                     /locus_tag="JNZ73_002804"
     CDS             complement(1578..1823)
                     /gene="moaD"
                     /locus_tag="JNZ73_002804"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HAY8733029.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFLPPVTGG"
     gene            complement(1816..2301)
                     /gene="moaC"
                     /locus_tag="JNZ73_002805"
     CDS             complement(1816..2301)
                     /gene="moaC"
                     /locus_tag="JNZ73_002805"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAY8733030.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2304..2816)
                     /gene="moaB"
                     /locus_tag="JNZ73_002806"
     CDS             complement(2304..2816)
                     /gene="moaB"
                     /locus_tag="JNZ73_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HAY8733031.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2838..3827)
                     /gene="moaA"
                     /locus_tag="JNZ73_002807"
     CDS             complement(2838..3827)
                     /gene="moaA"
                     /locus_tag="JNZ73_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAY8733032.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3815..3959)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4224..5132
                     /gene="yvcK"
                     /locus_tag="JNZ73_002808"
     CDS             4224..5132
                     /gene="yvcK"
                     /locus_tag="JNZ73_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HAY8733033.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5324..7345)
                     /gene="uvrB"
                     /locus_tag="JNZ73_002809"
     CDS             complement(5324..7345)
                     /gene="uvrB"
                     /locus_tag="JNZ73_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HAY8733034.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7924..8601)
                     /gene="bioD"
                     /locus_tag="JNZ73_002810"
     CDS             complement(7924..8601)
                     /gene="bioD"
                     /locus_tag="JNZ73_002810"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY8733035.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8594..9349)
                     /gene="bioC"
                     /locus_tag="JNZ73_002811"
     CDS             complement(8594..9349)
                     /gene="bioC"
                     /locus_tag="JNZ73_002811"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAY8733036.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9336..10490)
                     /gene="bioF"
                     /locus_tag="JNZ73_002812"
     CDS             complement(9336..10490)
                     /gene="bioF"
                     /locus_tag="JNZ73_002812"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HAY8733037.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10487..11527)
                     /gene="bioB"
                     /locus_tag="JNZ73_002813"
     CDS             complement(10487..11527)
                     /gene="bioB"
                     /locus_tag="JNZ73_002813"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAY8733038.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            11614..12903
                     /gene="bioA"
                     /locus_tag="JNZ73_002814"
     CDS             11614..12903
                     /gene="bioA"
                     /locus_tag="JNZ73_002814"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HAY8733039.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12881..13351
                     /locus_tag="JNZ73_002815"
     CDS             12881..13351
                     /locus_tag="JNZ73_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HAY8733040.1"
                     /translation="MKHFFANNGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTKSF
                     VVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTGYD
                     GAAPPKGETHRYIFTVHALDVERIDVDEGGSGAMVGFNVHFHSLASASITAMFS"
     gene            13666..13749
                     /locus_tag="JNZ73_002816"
                     /pseudo
     CDS             13666..13749
                     /locus_tag="JNZ73_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958651.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            13905..14906
                     /locus_tag="JNZ73_002817"
     CDS             13905..14906
                     /locus_tag="JNZ73_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8733041.1"
                     /translation="MHKSNCFDTARLVAAMMVLVSHHYALSGQPEPYLFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSILTYFLFGWILNDFSAE
                     YFSHDIVRKTISSIFMSQAPDADITSHLIHAGINGSLWTLPLEFLCYIITGVAVALLK
                     NGKAFIVILLVFVSLSLIGSVSENRDVMFSIPLWLYPLRGLAFFFGATMAMYEKSWNV
                     SNVKITVVSLLAMYAYASYGKGIDYTMTCYILVSFSTIAICTSVGDPLVKGRFDYSYG
                     VYIYAFPVQQVVINTLHMGFYPSMLLSAVTVLFLSHLSWNLVEKRFLTRSSPKLSLD"
     gene            complement(14940..>16080)
                     /locus_tag="JNZ73_002818"
     CDS             complement(14940..>16080)
                     /locus_tag="JNZ73_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HAY8733042.1"
                     /translation="YDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFD
                     NVMVNEVETAYLMQGLWHSKFIACQAGTCRVGLHFLGQCVSVSVSSCHFSRGNYSADE
                     SFGIRIQPQTYAWSSEAVRSEAIILDSETMCIGFKNAVYVHDCLDLHMEQLDLDYCGS
                     TGVVIENVNGGFSFSNSWIAADADGTEQFTGIYFRTPTSTQSHKIVSGVHINTANKNT
                     AANNQSIAIEQSAIFVFVSGCTLTGDEWAVNIVDINECVSFDKCIFNKPLRYLRSGGV
                     SVTDCYLAGITEVQKPEGRYNTYRGCSGVPSVNGIINVPVAVGATSGSAAIPNPGNLT
                     YRVRSLFGDPASSGDKVSVSVVTINVTRPSPVGVALPSMVEYLAI"
BASE COUNT         3823 a         4233 c         3999 g         4025 t
ORIGIN      
        1 aatgagccat ataccgttta atatcctggc ctcgtgctga aaatctaatt tattgctgtt
       61 ttgcgagtat gattccattc ttttagttgt ccttagaaaa aatattaata ttcaccatca
      121 ataaatttaa aaccacgcat acaaaatact tttgttttta tggatacgga aaacgattcc
      181 gctcgctcat aaggcatatt ttatacctgg tacttgttaa gtgtcgtttt tagtaagaaa
      241 aaaatcccga cacatggccg ggatagtgat ggtgaaaaag aattaacggc ggttgccgaa
      301 gatccgtaac aacatcagga acaggttgat gaagtccaga tacaaggtta acgcgccaag
      361 aatggaatat ttgcgcaggt tcgacgtgtc gcgggtatca atctgctcac ccatattttt
      421 caatttctga gtgtcatacg ccgtcaatcc gacaaagaca atcacgccga tgtaggtaac
      481 tgcccacatc aatgcttcgc ttttcagcca gaagttgacc agcgatgcca gcacaatgcc
      541 gattaacgcc ataaacagca tattgcccaa gccacttaaa tcgcgcttcg tggtgtaacc
      601 gtacaggctc attgcgccga acatcccggc agtaacgacg aaagtactgg cgatagaagc
      661 agcggtatag acaatgaata tactggaaag cgtaagaccc gtcagcgccg aataaagcat
      721 aaagagcatc gtcgttacac ctgcgctcag cttttgaatc atcgctgata acacaataac
      781 caatgctaat tgcgcgatga tcagaccgat taaaaagaca cggttagtga acaacagctc
      841 catcacggcc gcggaattag ccgcatacca ggcaacaaat gcggtcagca acaagccaac
      901 ggtcatccag ccatagactt gcgccatata agtttgcaag ccagcccggg gttgtacgat
      961 tgaatcagaa cgtgggaatc tgtccatgac gatctcctga agatataagg aatatcttaa
     1021 ggatactgca aaatgatgag gctgtgcatc gacgcagcgt aaacgcatgt actgagcggt
     1081 gaaattgccg gacgcagcgg tgccttatcc ggctaacaaa aaactaccag cgttttgccg
     1141 cctgcagatc gctctcccgg gcttcaaccc agcggtcgcc ttccggcgtg gcttcgcgct
     1201 tccagaacgg tgcgcgggtt ttgagataat ccataataaa ctgcccggct tcaaacgcac
     1261 tgctgcgatg cgcactggtg acaccgacaa aaacgatttc atcgcccggc cataattccc
     1321 cgatgcggtg aatcaccgtg acgcgcccca gcggccagcg gttacgcgct tcatcaacaa
     1381 tttctgccag tgctttttca gtcatccccg gatagtgttc gagggttaat gctttgacgc
     1441 tgtcgcccag gttatggttg cgcacctttc cggtgaaggt gactaccgca ccgtcttcgt
     1501 cacgctccgc cagccacggg tactcttctc ctacgctgaa cggctgcggg ccgacaacaa
     1561 ttttggtttc tgccatctta acctccggtt accggcggga ggaaagctac ttcgtcgccg
     1621 tcagtcagcg gatggtcaaa actcaccagc gtctggttga cggcagccag caatttgcca
     1681 tcttccagcg ccagcgccca gcgatcgctc tgcgcagcca ggtgctggcg taacgcttca
     1741 acggttgggt aatccgcagc cacttcggtt gcatctgttc ccaccaactc gcgcacctgg
     1801 gcgaaaaaaa gaactttaat catccgcttc caccttaaag tcacccgact tgccgccgct
     1861 cttcgccagc aaacgtaccg gaccaatcac catatctttt tgcaccgctt tgcacatgtc
     1921 ataaatggtc agcgccgcca cggaggccgc ggttaacgct tccatttcga caccggtttt
     1981 cccggtcagg cggcataagg tttctatacg cacccgattg tgctccggct cggcctgtaa
     2041 attgacttca actttgctga gcatcagcgg gtgacacagc gggatcagat cccaagtgcg
     2101 ttttgccgcc tgaataccgg caatacgcgc agtggcaaat acgtcgcctt tgtggtggcg
     2161 accatcaata atcatcgcca gcgtctcgct gcgcatggtg acaaaggctt ccgcccgcgc
     2221 ttcacgcacg gtttccgctt tggcggagac atccaccatg tgcgcttcgc cagcggcgtt
     2281 gatatgggtc agttgcgaca tacttatttc ttcaaatgtg gatggaaatt acacggacgc
     2341 gtacgggcat ccagctgcgg cgcgatgata ttttcccatg cggtacggca cgctttggtc
     2401 gaacccggca tggcgaaaat cagcgttttg ttagcgacgc ccgctaccgc acgagattgc
     2461 aacgtggaag tgccaatctc ttcaaacgac aacatacgga acacttcacc aaaaccttca
     2521 acctcacggt cgaacaacgg cagcagcgct tcgggagcct gatcaccttc cgtcaggcca
     2581 gtaccacccg taatcaatac cacttgtaca tcgtcgctgg cgatccacgc agatacctga
     2641 gcgcgaatag cgtagcggtt ttctttcaca atggctttat cgacaacgtg atggcccgct
     2701 tcttgcgccg aatcgcgcag atagtgaccg gaggtatcgt cttcttcacc gcgacgatta
     2761 gaaaccgtaa gaatagcaat acgggtcggg ataaattcag tgcttacctg actcatctga
     2821 tctctccttt tgacgtttta gccgccaatg tacgataagt tttgcgtaat accggtgttg
     2881 ttttgatgca ggaaatgggt ctgtttcttc tcccgcagcg ccgctgaaat acgcgcttcc
     2941 agcgcctgtt gctgggtatc gtcttccagc agatcgcgca ggttaacgcc gccttcacca
     3001 aacaggcaga gatggagttt accaatggag gaaacgcgca ggcggttgca agtggcgcag
     3061 aagtcttttt catacggcat gataaggcca atctctccgg cgtaatctgg atggcaaaag
     3121 acttgcgcgg gaccgtcgct gcgttgacgt aattggtgga tccagccgcg acgcagtagc
     3181 tcgtcacgca gaacctgacc agagatgtga tgcttacgga agagctcgct gccctcgccc
     3241 gtttccatca gttcgatgaa acgcagctgg atagggcgat gctggatcca gttcagaaag
     3301 gtgtcgagct ggtgatgatt aacatcacgc atcagcacgg tattgacttt gaccttctca
     3361 aaaccggcct caaatgcagc atcaatccct gccatgacct ggttgaattt atcctgcccg
     3421 gtaatagcgt gaaactggcg ggcgtccaaa ctgtcgacac tgacgttaat gccagtaagt
     3481 cccgcatcgc gccagttcgc cacatcgcgt tccagacggt aaccattggt ggtgaccgca
     3541 atctggcgga tagcgtcgtt ttcccgcaca gcggcgatga tatcggtaaa gtcgcggcgt
     3601 aaagacggct ctcctcctgt caggcgcact ttttcggtgc ccaggctggc gaaggcgcgc
     3661 gtaacccggc gaatttcatc gacggtaaga aagcctttat tggtgacgcc gctcggtttg
     3721 tagccatccg gcaggcagta ggtgcaacga aagttacaca catcggtaat cgacaggcgc
     3781 aagtagtaaa acttacgcgc aaatgcatca gtcagttgtg aagccatgta cacctttcca
     3841 gatacgggag gcgaagtcat ttcttccttc gccctggtgg caatctttac gagcattgtc
     3901 acggccaaag caccgtatca gttgacccag gtgcagaggc tagagtgttt agtggttaag
     3961 ccgatactag cgtgtaaatg agatttttac catccacact ttcgctatgc aatcatgtat
     4021 atagcgtcat gatcgtgcta tttcgctaca gaataaggga aaatggcggt tttactttga
     4081 tatacatcat cagggaggca tagcgtgcat cgtcctttag agatagaaga aaatagtttg
     4141 tatcctcaat attggcaggt taattgctgt ttccccgcaa tttgcgctac tgtagcgcgg
     4201 ataatctgac tccaggaaac tatatgcgca atcgtacgct ggctgatctt gatcgtgtcg
     4261 ttgctctcgg cggagggcat ggactgggac gcgttctctc atcactttcg tctttgggtt
     4321 ctcgtttaac gggtatcgtc accaccacgg ataatggtgg ctcgacgggg cgtattcgcc
     4381 gttcagaagg cggcattgct tggggcgata tgcgcaactg cctcaaccag ctgataacgg
     4441 aaccgagcgt cgcctccgcg atgtttgaat accgttttgg cggcaatggc gaactttccg
     4501 gtcataatct cggaaacttg atgttaaagg cgctggatca ccttagcgtg cggcctctgg
     4561 aagccatcaa tttaattcgt aatctgctga aagtggatac gcatttgatt ccaatgtcag
     4621 agcatcctgt tgatctgatg gcgattgacg atcaggggca tgaagtttac ggcgaggtca
     4681 atatcgacca gttaactacg ccgattcaag agttattgtt aacgcctaat gtacccgcaa
     4741 cgcgtgaggc ggttcacgct atcaatgaag cggatctcat cattattggg cctggcagtt
     4801 tttataccag cctgatgcca attctgctgc tgaaggaaat cgcccaggca ttacgccgca
     4861 cgccagcgcc gatggtttat atcggcaatc tggggcgtga gttgagttta cctgcggcta
     4921 atttgaagct ggaaagcaag ctggcaatta tggagcagta tgttggtaaa aaggtcattg
     4981 atgcggtcat cgtcgggcca aaagtggatg tctcggcggt gaaagagcgg attgtgatcc
     5041 aggaggtact ggaggccagc gatattccgt atcgtcatga ccgccagttg ttacataacg
     5101 cgctggaaaa ggcgttacag gctttaggtt aacggaaatc cgaaggaaaa ttccggcttc
     5161 ctattgaaga caaagtgcgc gttgtttatg ccggatgcgg cgtgaacgcc ttatccggcc
     5221 tacaaaccgc gcaaattcaa tatattgcgg agaaaatgta ggcctgataa gcgtagcgca
     5281 tcaggctgtt ttccgtttgt catcagtctt cttcgctatc ctgttacgat gcggcaataa
     5341 acaactcacg caactgatgc aactggtcac gaatttgtgc cgcttcttcg aactccagat
     5401 tctgcgcgtg ttgcatcatc agcccttcca gttcatggat tttctgctgc aacgctttcg
     5461 gcgacatatc catcggcaca ttatccggct caacaatcgg gcgcgatttt cctctgccct
     5521 tcgctttggt tttggcaatg ttctgcccca gcgccaggat atcgaccact ttcttgttca
     5581 agccttgcgg cgtaatgccg tgttcctcgt tgtacttctg ctgtttctca cggcgacgtt
     5641 cggtttcgcc aatcgctttc gccattgatg gagtgatctt atcgccgtag agaatcgctt
     5701 taccgttaac gttacgtgcc gcacgaccaa tggtctggat caacgaacgt tcggaacgca
     5761 ggaagccttc tttgtcagcg tcgaggatcg ccaccagcga cacttccggc atatccagac
     5821 cttcacgcag taagttgatc cctaccaata cgtcgaactc acccagacgc aagtcgcgga
     5881 taatctccat acgctcgacg gtgtcgatat ctgagtgaag ataacgcacg cgctcaccgt
     5941 gttcttcgag atattcggta agatcttccg ccattcgctt ggtcagtgtg gtgaccagca
     6001 cgcgttcgtt aattgccgct cgctgacgaa tctccgaaag aagatcatca acctgtgtcg
     6061 ccaccggccg cacttcgata atcgggtcaa gcaatccggt tggacgcacc acctgatcca
     6121 ccacatcgcc gccggatttt tccagctcgt aattacccgg cgtcgccgaa acatagatgg
     6181 tttgcggcgc taatgcttcg aactcttcaa acttaagcgg acggttatcc agcgctgatg
     6241 gcaggcggaa gccgtactcc accagtgtct ctttacgcgc ccggtcaccg cgatacatgc
     6301 cgccaatttg tggaatggtg acgtgagatt catcgacgac cagcagccca tcggcaggca
     6361 ggtaatcaaa cagcgtcggc ggtggctcac ccggtccacg accggagagg aagcgcgagt
     6421 agttttcaat ccccgaacag tagcccagct cgttcatcat ctccagatca aactgggtac
     6481 gctgggtcag ccgctgctct tccagcagtt tgttgttttc caacagcact ttgcgtctgg
     6541 cggccagttc ttctttgatc tcctccatcg cctgtacgat gcgctcgcgc ggtgtgacgt
     6601 agtgcgtttt cgggtagatg gtaaaacgtg gaatagtgga aacaatctgc ccggtcagcg
     6661 ggtcaaataa cgacaatcgt tccacttcct cgtcaaacag ttccacgcga agtgcaatgt
     6721 catccgattc tgccgggaag atatctatca cctcgccacg aacgcggaaa gtaccacgct
     6781 ggaatgcttg atcattacga gcgtattgca gctccgccag tcggcgcaga atcgcgcgct
     6841 gatcgataat cataccgacc gtgagatgga gcatcatctt gagatataaa tcaggatcgc
     6901 ccagaccata aatcgcggaa acagacgcca ctacaatcac atcacgccgc tccagcatcg
     6961 ctttggtggc ggacaaacgc atctgctcaa tatgttcgtt aaccgaggca tctttctcaa
     7021 tgaaggtgtc ggaactcggt acataggctt ccggctgata gtagtcgtag taggagacga
     7081 aatactccac cgcgttttcc gggaagaact ctttcatttc gccatacagc tgggccgcca
     7141 gcgttttgtt gggcgcaagt accatggttg ggcgctgaag gtcagcaatg acattggcaa
     7201 tggtgaaggt tttcccggag ccagtcacgc caagtaacgt ctggtgcgcc aggccatctt
     7261 ccagcccctc ttcgagacgt cgaatcgcct ctggctgatc gccagaaggt ttaaaagcgg
     7321 aattcagttt gaacggttta ctcatgagtc gctacctgaa ggagttgggc gggcaggtat
     7381 gtaattttac tcgtcgtact taattatgcc aacaaattat actggataaa aaaacagttc
     7441 atcaccataa tatttctgat acagcgtaaa ctccgctgtc aatcatgagc aaaatttact
     7501 ctgtggcgag ataaaactcc ggccttaccg ggttatcccc aaagcaacgg cttttttaac
     7561 atttgtcaag atgagtgatg acagttttat ggcaagagat gcctgtacag tgactgccat
     7621 tgcatttatc taaccagtta aaaaataaaa gatatttctt tgagccgtct ttaacgccaa
     7681 ttcgcgtgca agccgcgtat tctctcgctt gcctcgtgtt ttctaactct aatacacatg
     7741 gttatccaca ggaatagtgg ataactgtct ccagccccta tcccccgccg cctgggcaat
     7801 tcccacattc gccgcatgag gcggaagtaa aatttttttc gtgattattt tagaatttaa
     7861 ttggttaaat tgcagtcaat cgaagacgca atctcgctcg caatttaacc aaatacagaa
     7921 tggctacaac aaggcaaggt ttatgtactt tccggttgcc gcattttctg gattttctgc
     7981 aagccagggg atctctccca gcagcggcgc gggaatcatg cgggtgagcg tggtcatata
     8041 ttcagcgtga cgttttcccg gaggcgtaac atcgttcgcc acccaacccg ccagagtcag
     8101 tccggcgtgt tgtattacct gcgcagtcaa catcgcgtga ttaatacagc cgagtttcac
     8161 accaactacc agtatcaccg gcagttgttc ctgtgttacc caatctgcaa aagtgaaagt
     8221 gtcagaaagc ggcgtaaacc agccgccagc accttccact aacacccagt cagcctgttg
     8281 ttcaagcgcg cgtaatccgg cgctcattac caatgattct atcggcctgc cctcttgggc
     8341 gctgatgatg tgcggcgagg tgggttctgc gaaggtgtaa ggatttaccg ttgcgtaatc
     8401 cagctgcagg ctgctgttgt gctgtaacgc cagcgcgtca ctattacgta agccttccgg
     8461 ggtcttttcg ctgccagagg cgaccggttt ataacctgcc gtccgataac ctactgcctt
     8521 cgcggcttgt aaaagtgcac aactggcgac ggttttcccc acttcggtat ccgttccggt
     8581 gacaaaataa cgtttactca cgagcaatca ctcccaaaaa aagatgatac gtcagaggat
     8641 atcgcccctg ttgttgcggc caggccagtt gcaatcgctg caactgcgaa cgcgttaata
     8701 ttcgcgggtc gcgcccttca tgaagatgcg tagcaccgat gcctttcagc gaacgcatgg
     8761 cactgagcgc atcatcaaac catagtgtga tgagctgaat atgatgttga taacgcacgc
     8821 cgttcagcga ctcttcgatt tcatctggcg gtaaaaagcg attagcatgc ggacgctcgt
     8881 ccaccgcctg ccgcgcctga tgcagttcgg gtaacgatcc ctgcaccagc gtggtaaacg
     8941 cgaccacgcc tccggggcgc accacccgat acagctcgcg gagtgccgtg gataaattac
     9001 cgcaccactg caccgcgaga ttgctccatg caagatcgaa cgtcgcagtc gctaacggca
     9061 gggattcgat atctcccgcc agataatgat ctgcggcatc tttatggcgg gcttgcgcaa
     9121 gcattggcgg cgagagatct aacgccgtca cctgcgcgtg acgttcccgc cagtggcggc
     9181 tcatccagcc aggtccacaa cccgcgtcca gtacgtgggt gtatttacgc tgtggaagca
     9241 ttgccagtaa ggcgtcagca ctctggcgct gtagatctgc atgttgctca tagtgtgcgg
     9301 ctgcccgacc aaatgccgct gcaatggctt gtttattaac cgttgccatg cagcacctcc
     9361 agcagacggt cgatatcctg catttcatgc gcagcggtta gcgttaagcg aagtcgcgca
     9421 gtaccagcgg gtacggttgg cgggcgaatc gccgtgaccc agcagccttg ctggcgcagt
     9481 ttttctgcca gttgtaacgc acggctgtta tcaccgacga tcaatggctg gatggcgctg
     9541 catgaatcag ccagcgtaaa cggcaaatcc tgtactccgg cacgaaaacg cgcaatgagt
     9601 gacaccagtt tttcgcgccg tgcatccccc tcatcacggc gaatgaccgc cagcgacgca
     9661 cgtaatgcct gcgcctgagc gggcggcata ctggtgctgt agataaggtg gcgggcgaat
     9721 tgcagcagat aatccgccac cgtacttgag caaagcaccg cagccccact gacgccaaat
     9781 cctttgccaa aagtcactac cagcaattct ggttttacct tttgcagcca gcagctgccg
     9841 cgcccctgct ccccgataac gcccgtgccg tgggcatcat cgaccatcaa ccagccattg
     9901 tgctgttgcg ttacctgctg gatttccgcc agtggcgcgc tatcgccgtc catgctgaac
     9961 acgccttctg tcaccaccag ttgctgcccc ggacaggggg aagcaagcag tcgcgccaga
    10021 tgagcgacat cgttatgagt aaaacggcga agctgcgacg ggcttaaact ggcagcctcc
    10081 agcaatgagg catggctaag ccggtcggca acaatacggt cctctttcgc catcatcgcg
    10141 gcaatgactg cctgattcgc ggcaaaacca gagataaaca gcagcgcccg cgaatagcca
    10201 agccactcgg ccagttcttc ttccagcgcc tgatgcacca cgctataacc gctgacgtga
    10261 ccggagccgc cgctaccgat gccaaattgc tccgccccct gcttccaggc acggataatt
    10321 tgcggatgat ggcttaaacc taaataatcg ttactggaaa agttcagata ctggcgatca
    10381 tccgccacca gccagcgtcc ggctccttgc gccaccggat aacggcgacg cagggcatcg
    10441 gcagcacgcc gcgcatcgag cgccgcgttg attttctcct gccagctcat aatgctgccg
    10501 cgttgtaata ttcgtcggtg tccggggtca tcagcgcctg tccaagacgt tgctgttgtt
    10561 cgttatcccc tgccagcacg gcagtttgct gcggatttag ccccagtttg cggaacagtt
    10621 gcaggtcttt atcttcttcc ggattcggcg tggtcagcag tttgcaaccg tagaaaatcg
    10681 agtttgcgcc tgccataaag cacatcgcct gagtctgttc gttcatctgc tcgcgtccgg
    10741 cagaaaggcg cacgtaagag gtcggcatca tgatccgcgc tacggcaatg gtgcgaataa
    10801 aatcaaaggc atcaacgtca tcgttatcgg caagcggcgt gcctttcacc ttcaccagca
    10861 tgttgattgg tacgctttcc ggcggcgtcg gcaggtttgc cagttgcagc aataatccgg
    10921 cgcgatcttt taccgtttcg cctaagccca caatgccacc tgagcagact ttgatcccgg
    10981 catcgcgcac tttttccagc gtatcgaggc gttcctgata agtgcgtgtg gtgatgatat
    11041 tgccgtaaaa ctccggcgag gtgtccaggt tgtggttgta gtaatccagc ccggcgttcg
    11101 cgaggcgctg cgcctgagat tcactcaacg tgcccagcgt catacacgcc tccagcccca
    11161 tcgcttttac cccctgcacc atttgttcca ggtacggcat atcgcgttcg tggggattct
    11221 tccatgccgc gcccatacag aagcgcgtcg atcctgccgc tttcgctttg cgcgccgact
    11281 ccagcacctg ttcaacttcc atcaaccgct cggcttccag cccggttttg tagcgcgagc
    11341 tttgcgggca gtatttgcaa tcttccggac aagctccggt cttaatcgac agcaacgtgc
    11401 tgacctgcac ctgacgagga tcgaaatgct ggcgatgcac ctgctgcgct tcaaacagca
    11461 gatccagcaa cggtttttca aataattctg tgacttgcga caatgtccag cgtgggcggt
    11521 gagccatggg gcttctccaa aacgtgtttt ttgttgttaa ttcggtgtag gcttgtaaac
    11581 ctaaatcttt tcaatttggt ttacaagtcg attatgacaa cggacgatct tgcctttgac
    11641 caacgccata tctggcaccc atacacatcc atgacctccc ctctgccggt ttatccggtg
    11701 gcgagcgccg aaggttgcga gctaattttg tctgacggca gacgcctggt tgacggtatg
    11761 tcgtcctggt gggcggcgat ccacggctac aatcacccgc agcttaatgc ggcgatgaag
    11821 acgcaaattg atgccatgtc gcatgtgatg tttggcggta tcacccatgc gcccgccatc
    11881 gagctatgcc gcaaactggt ggcaatgacg ccgcaaccgc tggagtgcgt ttttctcgcg
    11941 gactccggtt ccgtagcggt ggaagtggcg atgaaaatgg cgttgcagta ctggcaagcc
    12001 aaaggcgaag cgcgccagcg ttttctgacc ttccgcaatg gttatcatgg cgataccttt
    12061 ggcgcgatgt cggtgtgcga tccggacaac tcaatgcaca gcctgtggaa aggctatctg
    12121 ccagaaaact tgtttgctcc cgccccgcaa agccgcatgg atggtgaatg ggatgaacgc
    12181 gatatggttg gttttgcccg cctgatggcg gcgcatcgtc atgaaatcgc agcggtgatc
    12241 attgagccga ttgtccaggg cgcaggcggg atgcgcatgt accatccgga atggttaaaa
    12301 cgaatccgca aaatgtgcga tcgcgaaggt atcttgctga ttgccgacga gatcgccacc
    12361 ggatttggtc gtaccggcaa actgtttgcc tgtgaatatg cagaaatcgc gccggacatt
    12421 ttgtgcctcg gtaaagcctt aaccggcggc acaatgaccc tttccgccac acttaccacg
    12481 cgcgaggttg cagaaaccat cagtaacggc gaagccggct gctttatgca tgggccaact
    12541 tttatgggca atccgctggc ctgcgcggca gcaaacgcca gcctggcgat tatcgaatcc
    12601 ggcgaatggc agcagcaggt ggcggctatt gaagtgcagc tgcgcgagca actggcacca
    12661 gcccgtgatg ccgaaatggt tgccgatgtg cgcgtactgg gggcaatcgg tgtggtcgaa
    12721 accactcgtc cggtgaatat ggcggcgctg caaaaattct ttgtcgaaca gggggtatgg
    12781 atccggcctt ttggcaaact gatttacctg atgccgccct atattattct cccgcaacag
    12841 ttgcagcgtc tgaccgcagc ggttaaccgc gcggtacagg atgaaacatt tttttgccaa
    12901 taatggcgat aagttgccgc atcgtcatgt ctttaacggc atgggttacg atggcgataa
    12961 tatttcaccg catctggcgt gggatgatgt tcctgcggga acgaaaagtt ttgttgtcac
    13021 ctgctacgac ccggatgcgc caaccggctc cggctggtgg cactgggtag ttgttaattt
    13081 acccgctgat acccgcgtat taccgcaagg gtttggctct ggtctggtag caatgccaga
    13141 cggcgttttg cagacgcgta ccgattttgg taaaaccgga tacgatggcg cagcgccgcc
    13201 gaaaggcgaa acccatcgct acatttttac cgttcacgcg ctggatgtag agcgtattga
    13261 tgtcgatgaa ggaggcagcg gcgcgatggt cgggtttaac gttcatttcc actctctggc
    13321 aagcgcatcg attaccgcga tgtttagtta atcactctgc cagatggcgc aatgccatct
    13381 ggtatcactt aaaggtatta aaaacaactt tttgtctttt taccttcccg tttcgctcaa
    13441 gttagtataa aaaagctgaa cgagaaacat aaaacgctgt aaatatcaat atgttatgaa
    13501 agatttagtc taaataatag actgcataat actacaaaac acaacatatc cagtcactat
    13561 gaatcaacta cttagatagt attagtgacc tgaggcagag cattagcgca aggtgatttt
    13621 ttgtcctctt gcgctaattt tttgtcaacg gactgggtta gttgaattta caaccatgct
    13681 aagtatctcg ataagcgcag agaaatgatg cagtggtggg cggattggct tgatgagaag
    13741 gttaggtagg caaatccatg cctgagcaga gccttgccct ctacctgagc ccatgctcat
    13801 tctttggcct caacgcgagc atgacctcac tggctggacc caaaatggac tatgacagaa
    13861 agatctgata tttttcctcg caaaaactta gaggctttat tttaatgcat aagagcaact
    13921 gctttgacac ggcaaggctt gtggcagcta tgatggttct ggtcagtcac cactacgcgc
    13981 tctctgggca gccggagccg tacctgttcg gctttgaaag tgctggcggc attgcagtta
    14041 tcatattctt ttcaatatca ggatatctga tttcaaaatc agctatcagg agtgattcct
    14101 ttattgattt catggctaaa agagcgagaa gaatattccc ggcgctggtc ccttgctcta
    14161 tactgacata ttttttattt ggatggatac ttaacgactt cagcgcagaa tattttagcc
    14221 atgacattgt caggaagacc ataagctcta tttttatgag tcaggctcca gatgctgaca
    14281 ttacatcaca tttaattcat gccggaatta acggcagttt gtggacccta ccgcttgagt
    14341 tcctgtgtta tataataacg ggagtagcag ttgccctact aaagaacgga aaagcattca
    14401 tagttatact gttggttttt gtttctcttt cattaattgg aagtgtgtca gaaaacaggg
    14461 atgtaatgtt ctctattccg ttatggctgt atcctttgcg tggactggcg ttcttttttg
    14521 gtgccactat ggcaatgtat gagaagtcat ggaatgtaag taatgttaaa attactgtag
    14581 tttctttgct tgctatgtac gcctacgcta gttatggcaa aggcattgat tatactatga
    14641 cttgctatat cctggtttca ttctcgacaa tagccatatg cacatccgtt ggcgacccac
    14701 tggttaaggg caggtttgat tactcgtatg gtgtgtacat ttatgcattc ccggttcagc
    14761 aggttgttat aaacacattg cacatgggtt tttacccgtc aatgttgtta agtgccgtga
    14821 ctgttctttt cttgtcccat ctttcatgga atctggttga aaaaagattc ctgacacgaa
    14881 gttcgcctaa attatccctg gattgaacaa taaaaggggc catatggccc ctaaaataat
    14941 cagatggcca gatactcaac cattgaaggc agcgcgactc ctactgggct tgggcgagta
    15001 acattaattg tcaccacgga aacactaacc ttgtcaccac ttgatgcagg gtcaccaaaa
    15061 aggcttctta ctctgtatgt caggttcccc gggttcggta tagctgcaga tccgcttgtg
    15121 gcacctaccg caactgggac attaatgatc ccatttacag acggtacgcc tgaacagcca
    15181 cgatacgtat tgtatctgcc ttccggtttc tgcacctcgg taatgccagc caaataacag
    15241 tcagtaactg acacgccacc gctacgaaga tagcgtagag gcttattgaa tatgcactta
    15301 tcgaaagaaa cacattcatt gatgtcgaca atatttacag cccattcatc accagttaac
    15361 gtacaaccac ttacaaagac gaagatcgcc gactgttcta tcgctatact ttgattgttt
    15421 gcagccgtgt ttttgtttgc agtgttgata tgaacaccac tgacaatttt atgtgactgc
    15481 gttgaggtcg gcgttctaaa atatatcccc gtaaattgtt cagtgccatc ggcatctgct
    15541 gctatccatg agtttgagaa agaaaatcct ccgtttacat tctctataac cacgcctgtt
    15601 gaaccgcaat aatctaaatc cagttgttcc atatgcaaat caaggcaatc atgaacatag
    15661 acggcatttt taaaaccaat gcacatggtc tcactgtcta aaattattgc ttctgaccta
    15721 actgcctctg acgaccacgc atacgtttga ggctgaatcc tgatgccaaa gctttcatca
    15781 gcagaataat ttcctctgct gaaatggcag gagctgacac taacacttac gcactggcct
    15841 aaaaagtgaa gaccgactct acaggttcca gcctgacatg ctataaattt tgagtgccag
    15901 agcccttgca ttaaataagc ggtttcaact tcattcacca ttacattatc aaatacccag
    15961 ccataaacct gtctgatgac agagtcttct gctgtaatgt ttgtgtcaat accattgcag
    16021 gtgttattac cagttactcg tatatttctc agataacggc cgatagtgtc tgaatcataa
//
LOCUS       DADMGC010000091        15915 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.091, whole genome shotgun sequence.
ACCESSION   DADMGC010000091 DADMGC010000000
VERSION     DADMGC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15915
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.091"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..396)
                     /locus_tag="JNZ73_002819"
     CDS             complement(<1..396)
                     /locus_tag="JNZ73_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HAY8733043.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTED"
     gene            complement(393..3077)
                     /locus_tag="JNZ73_002820"
     CDS             complement(393..3077)
                     /locus_tag="JNZ73_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8733044.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNTDVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            complement(3169..3669)
                     /locus_tag="JNZ73_002821"
     CDS             complement(3169..3669)
                     /locus_tag="JNZ73_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8733045.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            complement(3699..4415)
                     /locus_tag="JNZ73_002822"
     CDS             complement(3699..4415)
                     /locus_tag="JNZ73_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY8733046.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMPVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNSAQKLINAGNQRLKLLTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            4711..5487
                     /gene="exuR"
                     /locus_tag="JNZ73_002823"
     CDS             4711..5487
                     /gene="exuR"
                     /locus_tag="JNZ73_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HAY8733047.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            5832..6494
                     /locus_tag="JNZ73_002824"
     CDS             5832..6494
                     /locus_tag="JNZ73_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8733048.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            6498..6881
                     /gene="mzrA"
                     /locus_tag="JNZ73_002825"
     CDS             6498..6881
                     /gene="mzrA"
                     /locus_tag="JNZ73_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HAY8733049.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            7028..7382
                     /locus_tag="JNZ73_002826"
                     /pseudo
     CDS             7028..7382
                     /locus_tag="JNZ73_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            7420..7725
                     /locus_tag="JNZ73_002827"
     CDS             7420..7725
                     /locus_tag="JNZ73_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY8733050.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            7728..8132
                     /locus_tag="JNZ73_002828"
     CDS             7728..8132
                     /locus_tag="JNZ73_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733051.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            8122..8421
                     /locus_tag="JNZ73_002829"
     CDS             8122..8421
                     /locus_tag="JNZ73_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733052.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            8517..8999
                     /locus_tag="JNZ73_002830"
     CDS             8517..8999
                     /locus_tag="JNZ73_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY8733053.1"
                     /translation="MILSIDSNDANTAPLHKKTISSLSGAVESMMKKLEDVGVLVAHI
                     LMPILFITAGWGKITAYSGTQQYMEAMGVPGFMLPLVILLEFGGGLAILFGFLTRTTT
                     LFTAGFTLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW
                     "
     gene            9069..10055
                     /locus_tag="JNZ73_002831"
     CDS             9069..10055
                     /locus_tag="JNZ73_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY8733054.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            10348..10716
                     /locus_tag="JNZ73_002832"
     CDS             10348..10716
                     /locus_tag="JNZ73_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY8733055.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(10923..11819)
                     /locus_tag="JNZ73_002833"
     CDS             complement(10923..11819)
                     /locus_tag="JNZ73_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8733056.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYIMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            11924..12625
                     /locus_tag="JNZ73_002834"
     CDS             11924..12625
                     /locus_tag="JNZ73_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY8733057.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            12648..12812
                     /locus_tag="JNZ73_002835"
     CDS             12648..12812
                     /locus_tag="JNZ73_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733058.1"
                     /translation="MSKKSTKKRQLAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(13024..14316)
                     /locus_tag="JNZ73_002836"
     CDS             complement(13024..14316)
                     /locus_tag="JNZ73_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAY8733059.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGITVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGAKVSTSASAAWKAVLMALDDTAVTGNE
                     GIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(14344..15675)
                     /locus_tag="JNZ73_002837"
     CDS             complement(14344..15675)
                     /locus_tag="JNZ73_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY8733060.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTCWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
BASE COUNT         4147 a         4045 c         3682 g         4041 t
ORIGIN      
        1 atcttctgta catgaagatc tattagaatc cttaagattc gtctgaagcc ctttgccccc
       61 cttgcatcct agcggcgctt catcgctact tacaatggct gttttaaatc ctttgagttt
      121 taccggccaa cgcatcccac tgcgttgttc agttaaatag atttgtactg tcgatatatt
      181 tccattagct gtacaggttt gttggaggtc aggatacgaa cattcatacc acatatatcc
      241 attatttcct gccgacgctt catccttcca gaagtaataa tccgctctac cagatgccaa
      301 atcatacgta atttcactgg tcacgtcagg agctgcctga acgccagtga gcgtgccaaa
      361 caagcccaat atcgccgcaa tcaatcgatt tctcatggtg caacctccct ttgcgccgtc
      421 tgaacgtttc cacgtaattt aggctgtttc aactgtgcct gaacctgaat cgactccggt
      481 aattgtgaat aagtcaccac actacaagta acatctccgc tgtaacgaat gctggcgcgt
      541 ttctttacaa cactcagtgg acacttaagg aattgctgcc cggaaagcag atagagctct
      601 ttttcgctgc cgttaatttc aagtgtaaat ccgccatctt cagctaccag taacatggcg
      661 ctgttcagcc ccacaatcgg gaattttttc accgctgccg gcagtaataa acgaccgata
      721 tagttgtagt taaagctggc agacacttcg cgatagcgca gcttgccagg caacaacatt
      781 aattcacgat tccctgcacc gttaatgatg tttgttgtcc cctgctgatt gccctggcta
      841 ctgtcgttaa cctcaacaaa accagactga aatccaggat aagggaacaa cacactgtca
      901 ctttgaccaa gctgcaaagt tcggctgcct gccacccgcg cttcgagttg cagatcctga
      961 ctctcttcat cctgttcaga gaagcctttc acatccacca gtaccgcacc cagcaaatcg
     1021 tctgtaccac tggctcccaa attaacgcca tagcgactga cagccagcgt agagctataa
     1081 gtgcccgtaa aggccgaaag atgatcatga ttcttacggt cataactatc agatacggta
     1141 gcgtagacat ttccgtattg cccatcccaa cgcccgttaa ccgcgctgtt tatggtatcg
     1201 gtgttcaggc ctccgacggt tacgccaagt tctttatggc tgaatgaatc gttataaaag
     1261 gtatgcgata actgccatga cgtttgatcg ccatcctgtt cgctataacg ataatccgta
     1321 gacacattgg ttgaatggct gttgttattg ctatccatcg ttggcgtgtc agataacgaa
     1381 aacgataagt acaagccgtt gtcgtcgtta tcgccaccgt tgtcattacg atggaaaaca
     1441 ccgactgacg tactcaaaat ccagtcacgc atcacaatag cgcgggaagc gctaagttgt
     1501 acagtttcag agatactttg cgctgttgtc tggcgaagat agttattttc aaagctctgc
     1561 tgtttatccc aacgcaaact atcatcagtg ttattttgtg tgcaggtata acccagcatc
     1621 atattccagc cgacaaaatt cagtgaaact gtcgcggaaa tactttcata acagcttaag
     1681 gcgttatagc tctctctgct gtcggttgta caagcatcgc cgtcagagtt ggtccgataa
     1741 aagcccaacg tcggccatcc cggattactc cagttggcct gttgcatatc acctttgccg
     1801 ccgtcatcgt tacggaacac gctggcgctg attgcaagat tccccacccc gcctaaatct
     1861 gccgtccagt tattacctaa ctcgaaagca ctcacatcat cggcattcgc cagccctgcg
     1921 tagagctcat attgcggatg taatggcagg cgtactccta gctgataagc tgaactttca
     1981 tcatcagaaa cctgtgatgt agttttacct gcctgtaaga accattgcgc atttccgtca
     2041 gtcagaccgc cggttttggt aaacggcaca agctcggtgc gggtgagttg gttattttcg
     2101 tagactttta atgccacgct gtagctgcct ggcggaaagg aactggtatc aataaattgc
     2161 gaaccactat tgagataaaa cgatcccaaa agttgctcat tacgataagc gtcaacacga
     2221 gaattgcgcg aaagcagaac cattactggg gttccttgct ggctttgcgc ctggtttaaa
     2281 tagctcagtg tcgacccgat acgcatccca tcgattgcac cgagtggcag aaagttaaag
     2341 gtaaagttcc cgccttgtgc attaaatagt gtgcggttgt ccatgcgacc cgcctgcaca
     2401 taataacgac gtaaaaaatc ataacgataa tagagatcgc tgacgtcagt attactgtca
     2461 ctgacatcat ctgcatcata accgttgaaa ttccagtgtg caccaatata gctattttct
     2521 gttattccca gcgcaccgtt tccctgaata gacaacgatt ggtaatcatc ctgtgccaga
     2581 acattgatgt cttgctgatg tataaaagcg ttaacagtat ttttgtctgg atttaaatat
     2641 aatgacttcg catcgaaagc tgaattccat tgcggattaa taaacaacgt tgccgaactt
     2701 tcttcatcat tatagattat ttccagcgta tcggtattca gaaaaccaca ccctgagtca
     2761 gtacgtacat aaccgcaagc caattcaccg tggcgagcta gcggctgcga taatttttct
     2821 ttcactatat gcgcgatttt ctgatcgtct acatccagtt ccagcttgtt atatagactt
     2881 gctggatcaa gaaatgttac ggtatcaaga ttaaccattg cataatattt accgagactt
     2941 ttccctgaaa aataaacctc gatatactcc tgctgtccct gagcaataac ctcaaaccct
     3001 gcgggaattt tggttaatgt cgcctctgcc gggctgagac tcgccaggat caaaagagcc
     3061 agtaattttt tatccatggc aatcctgaaa tccataacaa atactaaaac agttaaaata
     3121 agggagggct acccctccct ttgttacgtg ttaattctgt tatccggatt aaatatcctg
     3181 agacagataa atgcttacta caccacggta gatgcctgtt tccagaacac ctttggtggt
     3241 ttgagcaaac atcaggtttt tagccagaga accgttagtt aacgcatcgg aagcaaagat
     3301 tttggttgcg gtaaaagtgg ttgccgcatc agctttaatt tcttcaccac cccaggttac
     3361 tgtcagcgga acaatcttgc tagcatccag gctttgaacc agttgcgctg gggacactaa
     3421 ctgcattttt acatctttgg taacgtcgtt agaccagatt ttggtcatca actggtaaga
     3481 ttgcagaccc tgccccggca gatattgcat ttctactgct ttcggcaacg cggtgttatc
     3541 ggtctgtgtc atatccagag cggcatcaac gttggccgtt acagtaatat ccttttgtac
     3601 tgccaatgcg gagcctgcaa cagaaaacat cgctgcgacc agaagagatt ttgcaaatac
     3661 ctttttcatt actattcctt cgtttattac aatgataatt aatggaccgc cagttcaaac
     3721 tcgccattat ctttggtgat ccagttatta tatttgacaa cgattttgtc gtagccagat
     3781 atgctttcaa gatttctttc ctgaccaggg aatatgtttt tatttacggt ctgtatctta
     3841 cagttttctt tgctattatt tttacaataa gcgatcgtta aaagctttaa gcgttgattc
     3901 cctgcattaa ttaatttttg ggcagaattt acttctaact tagctatagg ttgctgaggt
     3961 ggaacactaa caacgattcc ccaaattaag ttaactgtta actgcataga gagatctttt
     4021 ttatctgtgg caacgtcatc aatactggga accgcttcaa atttaacgcg ataatttttc
     4081 tctttttgct ctggttccat cgcaacaaaa cgtacggttt tgctactacc ggctggtaaa
     4141 gcgaatttag gtggcataac aaccagatga tttgcatcac cggatttaat ctcaacttca
     4201 ttttcctgag gcgtcgatgg attatcgata cggaatactg tcgccttgat gtactgaact
     4261 tcattacttt tagatattac gcgaacgttg ccttcaccct gactattaat ggaaacaggc
     4321 ataggataaa cagtcatatt tgactgtgcc aaaaaaggag cgaacacgaa taaaagcggc
     4381 gtaagccgta ataagagatt aattttcgcc accatataat tttgatctta caatatcgtt
     4441 tcaaaaaagt tggtgtatta gagatgtaga tttattgtaa gtcaatataa aaaagcattt
     4501 ttagttaaca ttaaatttaa aggctgggaa ttaacttact gaaatttagt agctttcaca
     4561 atgacgatac ttatttttta ggaatttata aatattcaca atattaattt aatacattaa
     4621 ttcaatcatc aaatttatat atgaccaatg gcatacccat gaataaagtg gtatgataac
     4681 ttcgtccgca ttccactccg ggagacgcat atggaaatca ctgaaccacg ccgtttgtat
     4741 caacaacttg ctgctgacct gaaagagcgc attgaacagg gcgtctatct ggtgggtgat
     4801 aaactgcctg ccgaacgctt tattgccgat gaaaagaacg tcagccgaac ggtagttcgt
     4861 gaagccatca tcatgctgga agttgaaggc tatgtggagg tgcgtaaagg ttcaggtatt
     4921 catgtggttt ccaaccagcc gcgtcatcag caggcggctg acaataatat ggaattcgcc
     4981 aattacggtc cgtttgagtt gcttcaggct cgccagctca tcgaaagtaa tattgccgag
     5041 ttcgcggcaa ctcaggtaac gaaacaagac atcatgaaac tgatggccat tcaggaacag
     5101 gcgcgcggcg aacaatgctt ccgtgattcc gagtgggatt tacagttcca cattcaggta
     5161 gccctggcga cgcagaactc cgccctggcg gccatcgttg aaaaaatgtg gacccagcgt
     5221 agccataacc cgtactggaa aaaactacac gaacacattg attcccgtac cgttgataac
     5281 tggtgtgatg accacgatca aattctcaag gcgttaattc gcaaagatcc tcatgctgct
     5341 aagctggcaa tgtggcagca tctggaaaac accaaaatca tgttatttaa cgaaaccagc
     5401 gacgatttcg agttcaatgc cgaccgctat ctgttcgccg aaaacccggt agtgcatctc
     5461 gatacggcca ccagcggcag caaatgaaat tcctgtccga caggcgctgc aatgcgcctg
     5521 tttgtctcgt cggtaagcga aaagtataaa gtgtcagcct gtgtaaatcc tctcgccacc
     5581 ctcccctgca atcagcaaaa tcagactcca cggacatgta attttgataa cgaacaacgt
     5641 tgacctttgt tacaattaga ttcaatttga atttatgctt ttgaatgctt tcttatctca
     5701 cgttttaaca gggaatagtt caggctgtgt tgatgtatca aacccgcaga acataccaaa
     5761 acagcaataa cattgcggta gtgcatcttt taaaaccagc gtggcgttaa ccgattcacc
     5821 aggaataatg aatggaactt ttgacccaat tgctgcaagc cctgtgggcg caggattttg
     5881 aaaccctggc caatccatcg atgattggca tgttgtattt tgtcttgttt gtaattttgt
     5941 tccttgaaaa tggcttgctt ccggcggcct ttttaccggg cgacagttta ctggtattgg
     6001 tcggcgtgtt gattgcgaaa ggcgcgatgg gttacccgca aacgattctg ctgctgaccg
     6061 ttgccgccag cctcggttgc tgggtcagct atattcaggg gcgatggctg ggcaataccc
     6121 gcaccgtaca aaactggcta tctcatttac ccgcgcatta tcatcaacgc gcacaccatc
     6181 tttttcataa acacggttta tcggcgctgt taattggtcg ctttattgca tttgtcagaa
     6241 cactgctgcc gacgattgcc gggttatcag ggctgaataa cgcgcgcttt cagttcttca
     6301 actggatgag cggtctgctg tgggtattga tcctgacaac tctgggttac atgctcggca
     6361 aaacgccggt atttttaaag tacgaggacc agctgatgtc atgcctgatg ctgctcccgg
     6421 tggtgctgct ggtgtttggc ctggcaggtt ctctggtcgt gttatggaaa aagaaatatg
     6481 gaaatcgggg gtaagggatg caaatacctc gcatgtcgct tcgccagcta gcctggtccg
     6541 gcactgtttt acttctggtc ggcacgctgt tgctggcctg gtccgcggtt cgccagcaag
     6601 agtctacgct ggcgattcgt gccgttcatc aaggcacaac gatgccagac ggtttttcga
     6661 tctggcatca ccttgacgct catggcattc ctttcaaaag tatcaccccc aaaaacgaca
     6721 ctctgttaat tacatttgac tccagcgacc agagcgccgc cgcaaaagcg gtcctcgaca
     6781 gaacattgcc ccatggctac atcattgcgc agcaggacaa taacagtcag gctatgcagt
     6841 ggctgacccg gttacgggat aactctcatc gcttcggata acttcctgtt atccgaaaca
     6901 tatcactcac tttggtgatt tcaccgtaac tgtctatgat taatgaggcg gtggttaaca
     6961 ctaccgcttc gttgtctgga caatcgttcc tttgtaatag gtccgaatca caatggaagg
     7021 ttcaagaatg aaataccgca tcgctttagc tgtttctctc tttgctctta gtgccggtag
     7081 ttatgccact accctgtgtc aggaaaagga gcaaaatatc cttaaggaga tcagctatgc
     7141 cgaaaaacac caaaaccaga atcgtattga cggtctgaat aaagccctga gtgaagtccg
     7201 ggccaactgt tcagatagcc agctgcgtgc cgatcatcac aagaaaatcg caaagcagaa
     7261 agatgaggtg gcggaacgcc agcaagattt agccgaggcg aagcaaaaag gcgatgccga
     7321 tagaacggaa actggcagaa gcgcaggaag agctgaaaaa gctggaagcg cgcgactact
     7381 aactcacaat agtcactact tactcacctg gagaaaacta tgtcgaaaga acacactacg
     7441 gaacatctgc gtgctgagtt gaaatccctt tccgatacgc tggaagaggt gcttagctca
     7501 tctggcgaga agtcgaaaga agagttgagt aagattcgta gcaaagcgga gcaggcactg
     7561 aaacagagcc gttatcgcct gggtgaaacc ggtgatgcca tcgccaaaca aacccgtgtc
     7621 gcggcggcgc gtgctgatga gtatgtgcgt gaaaatccgt ggaccggcgt gggaattggc
     7681 gctgcaatcg gtgtagtgct cggcgttctg ctgtcgcgtc gttaattatg gcggacactc
     7741 atcacgcgca agggcccggt aaaagcgttc tgggcatcgg gcagcgaatt gtttccatca
     7801 tggttgaaat ggtggaaaca cgtctgcggc tggcggtggt ggagctggaa gaggaaaaag
     7861 ctaatctctt tcaactttta ctgatgctgg gcctgacgat gcttttcgct gcatttggtc
     7921 ttatgagcct gatggtgcta attatttggg cggttgatcc gcaatatcgc ctgaatgcga
     7981 tgattgccac caccgtggtg ttgctgctac tggcactgat tggcggtatc tggacgttac
     8041 gtaaatcgcg taaatccacc ttgctgcgcc atacacgcca tgagctggca aacgatcggc
     8101 aactgctcga ggaggagtcc cgtgagcagt aaagtcgaac gtgaacgacg taaggcgcaa
     8161 ctgcttagcc agatccagca acaacggctg gatctttccg ccagtcgtcg tgaatggctg
     8221 gaggcaacag gcgcttacga tcgtcgctgg aatatgctgc taagtctgcg ctcctgggcg
     8281 ctggttggca gtagcgtgat ggcgatctgg acgattcgcc atcctaatat gctggtccgc
     8341 tgggccagac gcggttttgg cgtatggagc gcctggcgtc tggttaaaac gaccctcaag
     8401 cagcaacagc ttcgcggtta acttccctct ggccggagca attccggcct tatccctcaa
     8461 attttttgaa tatttttgac agttttcctt gctaacaatc atcattcacc acgtttatga
     8521 ttctctccat cgacagcaac gacgctaata ccgcgccctt gcacaaaaaa acaatcagca
     8581 gcctgagtgg cgcagtggag agtatgatga aaaaattaga agatgttggt gtactggtag
     8641 cgcacatttt aatgccgatt ctgtttatta ccgcaggctg gggaaaaatc accgcttact
     8701 ctggtaccca acaatatatg gaagcaatgg gtgtcccggg ttttatgctg ccgctggtga
     8761 ttctgcttga gttcggtggt ggtctggcaa tcctattcgg tttcctgact cgcaccacaa
     8821 ccctgtttac tgcgggcttt acgctgctga cggcattttt atttcacagc aactttgctg
     8881 aaggcgtcaa ctcgctgatg ttcatgaaaa acctgacaat ttctggcgga ttcctgctgc
     8941 tggcaattac cggtccgggc gcgtatagca tcgaccgcct gctgaataaa aagtggtaag
     9001 cacgcgctat acttaacgat aaaaaacgag gaggaagctc ctcgtttttg ctattggagg
     9061 agagaaaaat gggtcaactg attgacggcg tctggcatga cacctggtac gataccaaat
     9121 ctaccggcgg taaatttcaa cgttcagctt ccgcatttcg taactggctc actgccgatg
     9181 gcgcacctgg ccccactggc acaggcggtt ttatcgcaga gaaagatcgt tatcatctct
     9241 atgtttcact cgcctgcccg tgggcgcacc gcacgctgat catgcgcaaa ctcaaaggac
     9301 tggaaccgtt tatttccgtt tccgtagtga acccgctgat gctggaaaac ggctggacct
     9361 ttgatgacag ttttccggga gcaaccggag atacgctcta tcaacatgaa tttctgtatc
     9421 agctttatct ccacgccgat ccacactaca gcggacgagt tactgttccc gtgctgtggg
     9481 acaaaaagaa ccacaccatc gtcagcaacg aatcagcaga aatcatccgc atgttcaata
     9541 ccgcgtttga tgcgctgggc gcaaaagcgg gtgattacta cccaccagcc ctgcaaacga
     9601 aaattgacga acttaacggc tggatttatg acaccgttaa caacggcgtg tataaagctg
     9661 gttttgccac cagccagcaa gcctacgacg aggcggtggc gaaagtgttt gaatcgctgg
     9721 cgcgactgga gcagatttta ggccagcatc gttacctgac cggcaaccag ctaaccgaag
     9781 ccgatattcg cctgtggact acgctggtgc gttttgatcc agtgtatgtg acccacttca
     9841 agtgtgataa gcaccgcatc agcgattacc tgaatctgta tggcttcctg cgcgatatct
     9901 accagatgcc ggggatcgcc gaaactgtca atttcgatca catccgtaat cattacttcc
     9961 gcagccataa gaccatcaac cctacgggga ttatttcaat tggtccgtgg caggatctcg
    10021 atgaaccgca tggacgagat gttcgcttcg gttaaaaaaa ggcaccttac gaggtgcctc
    10081 tgataattag ctaagtgcat gttgttcatg tcggatgcgg cgtgaacgcc ttatcaaacc
    10141 tacaaagcgt ttatattcaa tatgttacaa tgaccgtgta ggccagataa gcgtagcgca
    10201 tctggcgatt ttgcgcttgc catcagtctt agcgcgcctt cacggcactt tattaattcc
    10261 ttgaacgaat atttactgcc atttcattca cgtctattct taatttgctg ctttaaaaac
    10321 aagtgattga gcaaaattga ggcaaaaatg gactggtatc tgaaagtact aaaaaattat
    10381 gtcggtttcc ggggtcgtgc gcggcgcaaa gagtactgga tgtttattct ggtcaacatc
    10441 atctttacgt tcgtgctggg gctgctggat aaaatgttag gctggcaacg cgctggtggc
    10501 gaaggcatcc tgacgacaat ctacggtatt ctggtgtttt taccgtggtg ggcggttcag
    10561 ttccgccgcc tgcacgacac cgaccgctcg gcgtggtggg cactgctatt cttaatcccg
    10621 tttatcggct ggcttattat catcgtcttt aactgccagg cgggtacgcc aggcgaaaac
    10681 cgctttggac cagatccaaa actggaacaa gaataatgat taagcacctt gtaaaaggtg
    10741 cttcaaattg ttgacaaagt gcgctttgtt tatgccggat gcggcgtaaa cgccttatcc
    10801 agcctacaaa ttgccaaaat tcaatatatt gcagtgactg cgtaggcctg ataagcgtag
    10861 cgcatcaggc agttttgcgt ttgtcagcag tcagaagcac cttgtgaaag gtgcttaata
    10921 gattattttc cagcgaacaa ctttggaatt tcccgcagac accaggattt cgcttccccc
    10981 atactgtcac gacgccaggc cataataata tcgatctcgc tggtcgattc cgggctgacg
    11041 acacgcaacc gcccttccgc aatatctttt tcgaccatcg gatacggcat cgtcgccacg
    11101 ccaagccccg ccagtaatgc ctgacgttta tcttcaatcg tgctcaccgt taagcgcggc
    11161 tgtttgtcca gcagctgtac ggtcaacacc gggcgctcac gagcggtatc cgctaccgca
    11221 attccacgat atttcacacg cgtcacttca gataacggtt ccggctcctg atgaatcggg
    11281 tgatcaggcg cggcaacgta gacgttcatt aacgtataga gcttgcgcga gttgatctcc
    11341 gacgaggaac gaaaatgcat atccggcgcg ataacgatat ccgcccgccc ctgctccagc
    11401 cgttcccacg cccccgccag cacttctgtg atgattgcca gttgggtatt ggcttttgcc
    11461 gccagtttgt cgattaacgg gaaaaaggca ggtgtcggta ccagcgcttc ggtcacaatg
    11521 gtgagatgcg tttcccaacc gcgcgcgaga gcttccgcat cagtagtcag tttatctgcg
    11581 gcttccagca aaacgcgccc tcgctccagc aacatcctcc cgacattggt gaatttggtg
    11641 cgatggcccg agcggtcaaa cagcaccaca tccagctctt cttccagttt ttgcatgatg
    11701 tagctaagtg cggaaggcac gcgtcccagc tcatccgctg ccgccgcaaa actgccccgg
    11761 cgatcgatcg catccataac ccgtagtgct tccagcgtta atgccctttc tttggccatt
    11821 tcgttctcat tcaggaaatt tgaacatacc gggcagaata tctggctaac aatgtagcgt
    11881 ccagcccctt accataaaag gaagtaaaga gaggtcaaga attatgatta ctacccgaac
    11941 tgccaggcag tgtggacaag cagactacgg atggttgcag gcccggtata ctttttcctt
    12001 tggacactac ttcgacccga aattgttagg ctatgcctcc ctgcgtgtgc ttaaccagga
    12061 agtgctggcc ccaggtgccg cctttcagcc gcgaacctat cccaaagtcg atattttaaa
    12121 tgtgattctg gatggagaag cagagtatcg cgacagcgaa ggcaatcatg ttcaggccag
    12181 cgccggagag gcgttgctgc tctctaccca gccgggtgtc agctatagcg aacacaatct
    12241 cagcaaagac aaaccgttaa cgcgaatgca gctttggctg gatacctgcc cgcagcgaga
    12301 gaatccgctg attcaaaagc tggcgcttaa tatggacaag cagcaattaa tcgcctcgcc
    12361 agagggtgcg atgggaagcc tgcaattacg ccaacaagtg tggctgcacc atatcgtgct
    12421 cgacaaaggc gaaagtgcga atttccagtt gcatgggcca cgcgcgtatt tgcaatcgat
    12481 tcacgggaaa tttcatgcgc ttacgcatca tgaagagaaa gcggcgctaa cctgcggtga
    12541 tggggcgttt attcgtgacg aggctaacat tacgctggtt gccgattccc cactgcgcgc
    12601 tttgctgata gatttgcctg tctagttgtt tttacaggga gatgatgatg agtaaaaaat
    12661 cgaccaaaaa gcgccagcta gcgaagcccg tggtggcgaa agaacctgct cgcaccgcca
    12721 aaaattttgg ctatgaagag atgttgagcg agctggaagc tatcgtcgcg gatgctgaaa
    12781 cgcgtttagc cgaggatgaa gctaccgcgt aaagacaggt tgccggatgc ggcgtaaacg
    12841 ccttatccgg cctacaaaac acaacaacat caacaaatta aacaccccgt aggccggata
    12901 agatgcgcca gcatcgcatc cggcatcagg tacaaaacac aacaacatca acaagttgag
    12961 caccccgtag gccggataag gtgcgccagc atcgcatccg gcacaatact caaaacctgg
    13021 cgtttatctg gccttgctcg ccataatctc gataatctgc cgatccgttt gctgcatcga
    13081 atggcttgct aacgcacaca ggttggcaat cgactgctca acatcatgcg ccacgatccc
    13141 ttcattgccg gtcacggcgg tatcatccag cgccattaac accgctttcc acgcagccga
    13201 agcactggtc gaaaccttcg caccatcgca aatcatgccg ctgacatcgc cgatcatact
    13261 gctgatcgcc atcgagatag tttcataacg cccatccacc agccatgcca tcccggcggc
    13321 ggcccccatt gctgcggtcg ttgcggcaca aagtgcagac aaacgcggta actggttatg
    13381 gatgtaaatt gcgctcaaat gagaaagcat cagcgcacgc gccagtcgtt catcatccgc
    13441 tccgaagtgt tctgctacaa ccaccacagg cattgttgcg gtgatcccct ggttacccga
    13501 gccggagtta ctcatagccg gaagcgtagc gccgcccata cgcgcatcgg atgccgcgct
    13561 ggtacgaatc acaatggatg aagagagatc tttcgccagc aaaccgcgct cgcactgttt
    13621 ttccagcgtc gcgccaatat gcagccccca cttaccgctc aaaccttcct gcgataacgc
    13681 acaatttagc ttcgcggaat cgagaataaa gcggatcgcc gcaaatggga cttcattgac
    13741 gaacttcagg atctcagcca gcgtcgttct ggaaagcacc gtcagcggcg actcttgctc
    13801 gccctctgcc acacacgcct gctgggtaaa caccacacca ttgtgcgtct caatatgcac
    13861 aatgttggta tgcccgccga cgatggtgac acacgcccac ttctcaccgt tccagacttt
    13921 ggcgcgtgag aagaggattt cattgcaagg ttcctggatc ttaacagaga ctttccccgc
    13981 cgccagcagt gctttggcat cggcaattgc ctgcgcagtt gcgtctttca gcacttccag
    14041 cccggcgttg gcatttccac ctaacgcccc cagcgccgcc gcaatcggca gccccaccat
    14101 tcccgtgccg ggaacggtga tgcccagacc gttcttcatc agatttggcg aaacccaggc
    14161 ttctacacgt tcaaccggac cttccagttc tgccgcagca accgccgccg ccagcgccag
    14221 tgaaatcggt tcagtacatc ccagcgccgg ttttacttcc tcctgaacgg cgaggatgta
    14281 acgctgccat aacggattta aagtcgaatc aaacatatta aaaaccttaa aatttcaggt
    14341 aaatcaagaa aatgccagga acagagaaac acaaagcaac aaaccagtga cgataatcag
    14401 gtacagagac atccctttgt atttgtgcaa tgccggtact ttgtaaacca gccacgccgg
    14461 gatcaggcac cctaccatgc cgaaaatcgg gctacagata gaggtgaagc tcaacaccgg
    14521 tgcgttcagt acgatggcgc tccaggccag caaaatggcg aaaatcatga tgccgcgctg
    14581 aacaagattt tcgttaatct tctcggcagg catcttgcga cgcaggatgt tcattacgat
    14641 cccttgcgtt gcttcgcgaa agcctaaata gacgccaaag aatgcagtca tgacagcaaa
    14701 gatattgaga atgacgctga ccactttcac ccacgctgcg ccgtcaccgc taataaactg
    14761 tgcggcaatc gccagcgcgg aaatattctg ctcgtaggct tttaccgctt cgtcatgtcc
    14821 catcgccagc gtgaacgaca cggcgtagaa aaagacggtg acaaacaaaa tgccaaacgc
    14881 gatattcatt gcccgcaatg ctttatgccg cgccacttca attgattttt cccgcgagcg
    14941 ataagagatc accatcgggc ttaacgtctg gataaacaga atcgacgtca gggtaaacgg
    15001 cagcgtaata atggcgtttt tcaccagcag ccccagcggc ggtagcgaac cgacgttata
    15061 cagatgccac attcccacca tcgacacgcc cagcgcagcg actaccagca gcttggtcag
    15121 caccatgtcg gtcgaaattt tgaataacaa tttctcgccg cgtgaagaga tcgccaccag
    15181 aatgcaaatc agtaccagac cataaaaggg actgtctgac agcaaccctt ccgtcacgcc
    15241 gaaggtatgc agataggaag cactatcgtt ggtgatggcg gtggaataga cgaacatcca
    15301 aatcaccagc attacgaaat agagcgcacc taacaggatg ccccagtttt tacctaaata
    15361 accgctaatg acactcgggt aatctttaca ttctggtgat tctgccagcg tattaataaa
    15421 caaccgctga aacagataca ttgccgggta accaatcacc gatgagagca aaaataccca
    15481 caatcccatc aaaccgacct gcaccgggag aaaaacaatc cccgcgccaa tcgccatccc
    15541 gatactcata atcacccagc aggtgtcagt actgtcgaat ttgatcgctt ctcgccactc
    15601 gctctcactc attccagcac gacccgccgg ggtcgaagcg tctgcaatga cgcctttatt
    15661 cgatgcaatt tccataattt ctcgctcaat attttgtagg gcttattatt tttttccgag
    15721 ccgcatcaag gcgatatgcg gtctacgttt ttgcaggcga gttattagaa taaagaaatg
    15781 atacgcgcga ggtcagagaa aatcttcaca atcaagactt taaaataaca gagatggata
    15841 agaattttct actaaattaa tcgcaagaga atattcattc tctatttgcg gcagatcaca
    15901 aaaaaaaggt gcaca
//
LOCUS       DADMGC010000092        15604 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.092, whole genome shotgun sequence.
ACCESSION   DADMGC010000092 DADMGC010000000
VERSION     DADMGC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.092"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..96
                     /locus_tag="JNZ73_002838"
     CDS             <1..96
                     /locus_tag="JNZ73_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY8733061.1"
                     /translation="IHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            279..473
                     /gene="bfd"
                     /locus_tag="JNZ73_002839"
     CDS             279..473
                     /gene="bfd"
                     /locus_tag="JNZ73_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HAY8733062.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            546..1022
                     /gene="bfr"
                     /locus_tag="JNZ73_002840"
     CDS             546..1022
                     /gene="bfr"
                     /locus_tag="JNZ73_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAY8733063.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1019..1486)
                     /locus_tag="JNZ73_002841"
     CDS             complement(1019..1486)
                     /locus_tag="JNZ73_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAY8733064.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1865..2176
                     /gene="rpsJ"
                     /locus_tag="JNZ73_002842"
     CDS             1865..2176
                     /gene="rpsJ"
                     /locus_tag="JNZ73_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAY8733065.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2209..2838
                     /gene="rplC"
                     /locus_tag="JNZ73_002843"
     CDS             2209..2838
                     /gene="rplC"
                     /locus_tag="JNZ73_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAY8733066.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2849..3454
                     /gene="rplD"
                     /locus_tag="JNZ73_002844"
     CDS             2849..3454
                     /gene="rplD"
                     /locus_tag="JNZ73_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAY8733067.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3451..3753
                     /gene="rplW"
                     /locus_tag="JNZ73_002845"
     CDS             3451..3753
                     /gene="rplW"
                     /locus_tag="JNZ73_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAY8733068.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3771..4592
                     /gene="rplB"
                     /locus_tag="JNZ73_002846"
     CDS             3771..4592
                     /gene="rplB"
                     /locus_tag="JNZ73_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAY8733069.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4609..4887
                     /gene="rpsS"
                     /locus_tag="JNZ73_002847"
     CDS             4609..4887
                     /gene="rpsS"
                     /locus_tag="JNZ73_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAY8733070.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4902..5234
                     /gene="rplV"
                     /locus_tag="JNZ73_002848"
     CDS             4902..5234
                     /gene="rplV"
                     /locus_tag="JNZ73_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAY8733071.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5252..5953
                     /gene="rpsC"
                     /locus_tag="JNZ73_002849"
     CDS             5252..5953
                     /gene="rpsC"
                     /locus_tag="JNZ73_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAY8733072.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5966..6376
                     /gene="rplP"
                     /locus_tag="JNZ73_002850"
     CDS             5966..6376
                     /gene="rplP"
                     /locus_tag="JNZ73_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAY8733073.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6376..6567
                     /gene="rpmC"
                     /locus_tag="JNZ73_002851"
     CDS             6376..6567
                     /gene="rpmC"
                     /locus_tag="JNZ73_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAY8733074.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6567..6821
                     /gene="rpsQ"
                     /locus_tag="JNZ73_002852"
     CDS             6567..6821
                     /gene="rpsQ"
                     /locus_tag="JNZ73_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAY8733075.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6986..7357
                     /gene="rplN"
                     /locus_tag="JNZ73_002853"
     CDS             6986..7357
                     /gene="rplN"
                     /locus_tag="JNZ73_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAY8733076.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7368..7682
                     /gene="rplX"
                     /locus_tag="JNZ73_002854"
     CDS             7368..7682
                     /gene="rplX"
                     /locus_tag="JNZ73_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAY8733077.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7697..8236
                     /gene="rplE"
                     /locus_tag="JNZ73_002855"
     CDS             7697..8236
                     /gene="rplE"
                     /locus_tag="JNZ73_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAY8733078.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8251..8556
                     /gene="rpsN"
                     /locus_tag="JNZ73_002856"
     CDS             8251..8556
                     /gene="rpsN"
                     /locus_tag="JNZ73_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAY8733079.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8590..8982
                     /gene="rpsH"
                     /locus_tag="JNZ73_002857"
     CDS             8590..8982
                     /gene="rpsH"
                     /locus_tag="JNZ73_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAY8733080.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8995..9528
                     /gene="rplF"
                     /locus_tag="JNZ73_002858"
     CDS             8995..9528
                     /gene="rplF"
                     /locus_tag="JNZ73_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAY8733081.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9538..9891
                     /gene="rplR"
                     /locus_tag="JNZ73_002859"
     CDS             9538..9891
                     /gene="rplR"
                     /locus_tag="JNZ73_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAY8733082.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9906..10409
                     /gene="rpsE"
                     /locus_tag="JNZ73_002860"
     CDS             9906..10409
                     /gene="rpsE"
                     /locus_tag="JNZ73_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAY8733083.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10413..10592
                     /gene="rpmD"
                     /locus_tag="JNZ73_002861"
     CDS             10413..10592
                     /gene="rpmD"
                     /locus_tag="JNZ73_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAY8733084.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10596..11030
                     /gene="rplO"
                     /locus_tag="JNZ73_002862"
     CDS             10596..11030
                     /gene="rplO"
                     /locus_tag="JNZ73_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAY8733085.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11038..12369
                     /gene="secY"
                     /locus_tag="JNZ73_002863"
     CDS             11038..12369
                     /gene="secY"
                     /locus_tag="JNZ73_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAY8733086.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12401..12517
                     /gene="rpmJ"
                     /locus_tag="JNZ73_002864"
     CDS             12401..12517
                     /gene="rpmJ"
                     /locus_tag="JNZ73_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAY8733087.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12664..13020
                     /gene="rpsM"
                     /locus_tag="JNZ73_002865"
     CDS             12664..13020
                     /gene="rpsM"
                     /locus_tag="JNZ73_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAY8733088.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13037..13426
                     /gene="rpsK"
                     /locus_tag="JNZ73_002866"
     CDS             13037..13426
                     /gene="rpsK"
                     /locus_tag="JNZ73_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAY8733089.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13460..14080
                     /gene="rpsD"
                     /locus_tag="JNZ73_002867"
     CDS             13460..14080
                     /gene="rpsD"
                     /locus_tag="JNZ73_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAY8733090.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14106..15095
                     /gene="rpoA"
                     /locus_tag="JNZ73_002868"
     CDS             14106..15095
                     /gene="rpoA"
                     /locus_tag="JNZ73_002868"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAY8733091.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15136..15519
                     /gene="rplQ"
                     /locus_tag="JNZ73_002869"
     CDS             15136..15519
                     /gene="rplQ"
                     /locus_tag="JNZ73_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAY8733092.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4014 a         3670 c         4159 g         3761 t
ORIGIN      
        1 atccacccga tcgcgatgga cgacggtctg cgtttcgcaa tccgtgaagg cggccgtacc
       61 gttggcgcgg gcgttgttgc taaagttctg ggctaattac acgttaatta gttttgaatt
      121 gaaaagggcg cttcggcgcc ctttttgctg cccgtaattt gagctcgaaa tatttagtcg
      181 taattttgct cgctgaacta tggcgcaaat aaaactgtgc tattgtaatc ataaccattc
      241 tcatttacac tttgtgcaga atttgaacgg gagcaatcat gtacgtttgt ttgtgtaatg
      301 gtgtaagtga caaaaaaatt cgccaggcag tccgtcagtt tcatccgcaa tctttccaac
      361 aattgcgtaa gtttattcct gtgggaaatc aatgcggtaa gtgcattcgc gccgcgcgcg
      421 aagtgatgca ggacgagttg atgcagatgc cagaatttaa agagattgcc tgaagcccac
      481 gcttattttt tgacatcccg ctagccccat ctacgctcta atagtggaag cggagggact
      541 ataaaatgaa aggtgatgtt aaaatcataa attatctcaa taaactattg ggaaatgagc
      601 ttgtcgcaat caatcagtat tttctccatg cccggatgtt taaaaactgg ggcctgatgc
      661 gtctcaatga cgtcgaatac catgaatcca tcgatgagat gaaacacgct gataaatata
      721 tcgagcgtat tttgttttta gaaggaatcc ccaacttaca ggatttaggc aaactgggga
      781 ttggtgaaga tgtcgaggag atgctgcaat ctgacctgcg attagaactg gaaggcgcga
      841 aggatctgcg tgaggctatc gcatatgccg acagcgttca tgactatgtt agccgcgata
      901 tgatgatcga gatccttgct gatgaagaag gtcatatcga ctggctcgaa actgaactgg
      961 atctcatcgg taaaatcggc ctacaaaatt acctgcaatc acaaattaaa gttaaagatt
     1021 aacggcctat cagcaaacta tcccagccta taacgaaacc cgcagccgcc agaaatggcc
     1081 caaaaggcag cgggtttttt aatgattctt ttcctctcat gagcagacca ataactacgg
     1141 ctccgcaagc gaatgaggca gcaaggaaaa ccagtcgtgg taagaaggcc catgaatgcc
     1201 aggcgccaag ggcggcgaga aacttcacat caccatagcc taatccttct ttatgtcgca
     1261 gtatgcgata cccccagtaa atgacagcga atgtgccgta accaacaatt gccccccata
     1321 acgcatcggc taaaccatca ggatgacaaa cttgatagaa caaaagacct gaccagagta
     1381 acgggcacgt aaatctgtca ggtaacaagc catgctttgc atcccagaaa aaaagtaggg
     1441 ctgataaaca agcgtacaaa attagaaagg gaagggtagt gtccataaaa catcctctgt
     1501 aattggagta tcaccatcga ataactccgg atgctcagca acagagagag cgctgatttg
     1561 ttaacctgat tccggtgaaa aacgctatct tcacaacctt acaaatttcg tggaatgcag
     1621 ctcgcaatct gcgcgttgcg attaatttct acccgatgct tgcgtcaggc tcagatttgc
     1681 gtataatgcg cgggcttgtc gtagttgaca gcaggttcaa tctgaacctc agttagccga
     1741 atttggctac ctaacaatgc tcccaatcgg ggagctacgt aagaacggtt acactctccc
     1801 atcaatcgta atgggtctga ggagtaatca ttttcgttta taaaataatt ggagctctgg
     1861 tctcatgcag aaccaaagaa tccgtatccg cctgaaagcg tttgatcatc gtctgatcga
     1921 tcaagcaacc gcggaaatcg tcgagactgc caagcgcact ggtgcgcagg tccgtggtcc
     1981 gatcccgctg ccgacacgca aagagcgctt cactgttctg atctccccgc acgtcaacaa
     2041 agacgcgcgc gatcagtacg aaatccgtac tcacttgcgt ctggttgaca tcgttgagcc
     2101 aaccgagaaa accgttgatg ctctgatgcg tctggatctg gctgccggtg tagacgtgca
     2161 gatcagcctg ggttaatcag gtcattgagc gattgagagg ttgaaacaat gattggttta
     2221 gtcggtaaaa aagtgggtat gacccgtatc ttcacagaag acggcgtttc tatcccagta
     2281 accgtaatcg aagttgaagc aaaccgcgtt actcaggtta aagacctggc taacgatggc
     2341 taccgtgcta ttcaggtgac caccggtgct aaaaaagcta accgtgtgac caagcctgaa
     2401 gctggccact tcgctaaagc tggcgtagaa gctggccgtg gtctgtggga attccgcctg
     2461 gctgaaggcg aagagttcac tgtaggtcag agcattagcg ttgaactgtt tgctgacgtt
     2521 aaaaaagttg acgtaactgg cacctctaaa ggtaaaggtt tcgcaggtac cgttaagcgc
     2581 tggaacttcc gtacccagga cgctactcac ggtaactcct tgtctcaccg cgttccgggt
     2641 tctatcggtc agaaccagac tccgggcaaa gtgttcaaag gcaagaaaat ggcaggtcag
     2701 atgggtaacg aacgtgtaac cgttcagagc cttgacgtag tacgcgttga cgctgagcgc
     2761 aacctgctgc tggttaaagg tgctgtcccg ggtgcaaccg gtagcgacct gatcgttaaa
     2821 ccagctgtga aggcgtaagg agatagcaat ggaattagta ttgaaagacg cgcagagcgc
     2881 gctgactgtt tccgaaacta ccttcggtcg tgatttcaac gaagcgctgg ttcaccaggt
     2941 tgttgttgct tatgcagctg gtgctcgtca gggtactcgt gctcagaaga ctcgtgctga
     3001 agtaactggt tccggtaaaa aaccgtggcg ccagaaaggc accggccgtg cgcgttctgg
     3061 ttctatcaag agcccgatct ggcgttctgg tggcgtgacc tttgctgctc gtccgcagga
     3121 ccacagtcaa aaagttaaca agaagatgta ccgcggcgcg ctgaaaagca tcctgtccga
     3181 actggtacgt caggatcgtc tgatcgttgt cgagaagttc tctgtagaag cgccgaaaac
     3241 taagctgctg gcacagaaac tgaaagacat ggctctggaa gatgtgctga tcatcaccgg
     3301 tgagctggac gaaaacctgt tcctggctgc gcgcaacctg cacaaggttg acgtacgcga
     3361 tgcaactggt atcgacccgg ttagcctgat cgccttcgac aaagtcgtaa tgactgctga
     3421 tgctgttaag caagttgagg agatgctggc atgattcgtg aagaacgtct gctgaaggtg
     3481 ctgcgtgcac cgcacgtttc tgaaaaagcg tctactgcga tggaaaaatc caacaccatc
     3541 gtactcaaag ttgctaaaga cgcgaccaaa gcagaaatca aagctgctgt gcagaaactg
     3601 tttgaagtcg aagtcgaagt cgttaacacc ctggtagtta aagggaaagt taaacgtcac
     3661 ggacagcgta tcggtcgtcg tagcgactgg aaaaaagctt acgtcaccct gaaagaaggc
     3721 cagaatctgg acttcgttgg cggcgctgag taagtcggag gagtaataca atggcagttg
     3781 ttaaatgtaa accgacatct ccgggtcgtc gccacgtagt taaagtggtt aaccctgagc
     3841 tgcacaaggg caaacctttt gctccgttgc tggaaaaaaa cagcaaatcc ggtggtcgta
     3901 acaacaatgg ccgtatcacc actcgtcata tcggtggtgg ccacaagcag gcttaccgta
     3961 ttgttgactt caaacgcaac aaagacggta tcccggcagt tgttgaacgt cttgagtacg
     4021 atccgaaccg ttccgcgaac atcgcgctgg ttctgtacaa agacggtgaa cgccgttaca
     4081 tcctggcccc taaaggcctg aaagctggcg accagattca gtctggcgtt gatgctgcaa
     4141 tcaaaccagg taacaccctg ccgatgcgca acatcccggt tggttctact gttcataacg
     4201 tagaaatgaa accaggtaaa ggcggtcagc tggcacgttc cgctggtact tacgttcaga
     4261 tcgttgctcg tgatggtgct tatgtcaccc tgcgtctgcg ttctggtgaa atgcgtaaag
     4321 tagaagcaga ctgccgtgca actctgggcg aagttggcaa tgctgagcat atgctgcgcg
     4381 ttctgggtaa agcaggtgct gcacgctggc gtggtgttcg tccgaccgtt cgcggtactg
     4441 cgatgaaccc ggtagaccac ccacatggtg gtggtgaagg tcgtaacttt ggtaagcacc
     4501 cggtaactcc gtggggcgtt cagaccaaag gtaagaagac ccgcagcaac aagcgtactg
     4561 ataaattcat cgtacgtcgc cgtagcaaat aattttagag gataagccat gccacgttct
     4621 ctcaagaaag gtccttttat tgacctgcac ttgctgaaga aggtagagaa agcggtggaa
     4681 agcggagaca agaagcccct gcgcacttgg tcccgtcgtt caacgatctt tcctaacatg
     4741 atcggtttga ccatcgctgt ccataatggt cgtcagcacg ttccggtatt tgtaaccgac
     4801 gaaatggtcg gtcacaaact gggtgaattc gcaccgactc gtacttatcg cggccacgct
     4861 gctgataaaa aagcgaagaa gaaataaggt aggaggaaga gatggaaact attgctaaac
     4921 atcgccatgc tcgttcttct gctcagaagg ttcgccttgt tgctgacctg attcgcggta
     4981 agaaagtgtc gcaggctctg gatattttga cctacaccaa caagaaagcg gctgtacagg
     5041 tcaagaaagt tctggaatct gccattgcta acgctgaaca caacgatggc gctgacattg
     5101 acgatctgaa agttacgaaa attttcgtag acgaaggccc gagcatgaag cgcattatgc
     5161 cgcgtgcaaa aggtcgtgca gatcgtatcc tgaagcgcac cagccacatc actgtggttg
     5221 tgtccgatcg ctgagactct ggagactagc aatgggtcag aaagtacatc ctaatggtat
     5281 tcgcctgggt attgtaaaac catggaactc tacctggttt gcgaacacca aagaattcgc
     5341 tgacaacctg gacagcgatt ttaaagtacg tcagtacctg actaaggaac tggctaaagc
     5401 gtccgtatct cgtatcgtta tcgagcgtcc ggctaagagc atccgtgtaa ccattcacac
     5461 tgctcgcccg ggtatcgtta tcggtaagaa aggtgaagac gtagaaaaac tgcgtaaggt
     5521 cgtagcggac atcgctggcg ttcctgcaca gatcaacatc gccgaagttc gtaagcctga
     5581 actggacgca aaactggttg ctgacagcat cacttctcag ctggaacgtc gcgttatgtt
     5641 ccgtcgtgct atgaagcgtg ctgtacagaa cgcaatgcgt ctgggcgcta aaggtattaa
     5701 agttgaagtt agcggccgtc tgggcggcgc ggaaatcgca cgtaccgaat ggtaccgcga
     5761 aggtcgcgta ccgctgcaca ctctgcgtgc tgacatcgac tacaacacct ctgaagcgca
     5821 caccacttac ggtgtaatcg gcgttaaagt gtggatcttc aaaggcgaga tcctgggtgg
     5881 tatggctgct gttgaacaac cggaaaaacc ggctgctcag cctaaaaagc agcagcgtaa
     5941 aggccgtaaa taaggagcgt cgctgatgtt acaaccaaag cgtacaaaat tccgtaaaat
     6001 gcacaaaggc cgtaaccgcg gtctggcgca gggtacggat gttagcttcg gcagcttcgg
     6061 tctgaaagct gttggccgtg gtcgtctgac tgcccgtcag atcgaagcag cacgtcgtgc
     6121 tatgacccgt gcagttaagc gtcaaggtaa gatctggatc cgtgtgttcc cggacaaacc
     6181 gatcactgaa aagccgctgg cagtgcgtat gggtaaaggt aaaggtaacg tggagtattg
     6241 ggttgccttg attcagccgg gtaaagtcct gtatgaaatg gacggtgttc cggaagagct
     6301 ggcccgtgaa gcattcaagc tggcagcagc gaaactgccg attaaaacca cctttgtaac
     6361 taagacggtg atgtaatgaa agcaaaagag ctgcgtgaga agagcattga agagctgaac
     6421 accgagctgc tgaacctgct gcgtgagcag ttcaacctgc gtatgcaggc tgcaagtggc
     6481 cagctgcaac agtctcacct gttgaagcaa gtgcgtcgcg atgtcgcacg cgttaagact
     6541 ttactgaacg agaaggcggg tgcgtaatga ccgataaaat ccgtactctg caaggtcgcg
     6601 ttgttagcga caaaatggag aaatccattg ttgttgctat cgaacgtttt gtgaaacacc
     6661 cgatctacgg taaattcatc aagcgtacga ccaaactgca cgtacatgac gagaacaacg
     6721 aatgcggtat cggtgacgtg gttgaaatcc gcgaatgccg tccgctgtcc aagactaaat
     6781 cctggacgct ggttcgcgtt gtagagaaag cggttctgta atacagtaca ctctctcgat
     6841 atgaataaac ggctcagaaa tgagccgttt attttttcta cccatatcct tgaagcggtg
     6901 ttataatgcc gcgccctcga tatggggatt tttaacgacc tgattttcgg gtctcagtag
     6961 tagttgacat tagcggagca ctaaaatgat ccaagaacag actatgctga acgtcgccga
     7021 caactccggt gcacgtcgcg taatgtgtat caaggttctg ggtggctcgc accgtcgcta
     7081 cgcaggcgta ggcgacatca tcaagatcac catcaaagaa gcaattccgc gtggtaaggt
     7141 caaaaaaggt gatgtgctga aggcggtagt ggtgcgcacc aagaagggtg ttcgtcgccc
     7201 ggacggttct gtcattcgct tcgatggtaa tgcttgtgtt cttctgaaca acaacagcga
     7261 gcagcctatc ggtacgcgta tttttgggcc ggtaactcgt gagcttcgta gtgagaagtt
     7321 catgaaaatt atctctctgg caccagaagt actctaagga gcgaatcatg gcagcgaaaa
     7381 tccgtcgtga tgacgaagtt atcgtgttaa ccggtaaaga taaaggtaaa cgcggtaaag
     7441 ttaagaatgt cctgtcttcc ggcaaggtca ttgttgaagg tatcaacctg gttaagaaac
     7501 atcagaagcc ggttccggcc ctgaaccaac cgggtggcat cgttgaaaaa gaagccgcta
     7561 ttcaggtttc caacgtagca atcttcaatg cggcaaccgg caaggctgac cgtgtaggct
     7621 ttagattcga agacggcaaa aaagtccgtt tcttcaagtc taacagcgaa actatcaagt
     7681 aatttggagt agtacgatgg cgaaactgca tgattactac aaagacgaag tagttaaaaa
     7741 actcatgact gagtttaact acaattctgt catgcaagtc cctcgggtcg agaagatcac
     7801 cctgaacatg ggtgttggtg aagcgatcgc tgacaaaaaa ctgctggata acgcagcagc
     7861 agacctggca gcaatctccg gtcaaaaacc gctgatcacc aaagcacgca aatctgttgc
     7921 aggcttcaaa atccgtcagg gctatccgat cggctgtaaa gtaactctgc gtggcgaacg
     7981 catgtgggag ttctttgagc gcctgatcac tattgctgta cctcgtatcc gtgacttccg
     8041 tggcctgtcc gctaagtctt tcgacggtcg tggtaactac agcatgggtg tccgtgagca
     8101 gatcatcttc ccagaaatcg actacgataa agtcgaccgc gttcgtggtt tggatattac
     8161 cattaccact actgcgaaat ctgacgaaga aggccgcgct ctgctggctg cctttgactt
     8221 cccgttccgc aagtaaggta gggttactaa atggctaagc aatcaatgaa agcacgcgaa
     8281 gtaaaacgcg tagctttagc tgataaatac ttcgcgaaac gcgctgaact gaaagcgatc
     8341 atctctgatg tgaacgcttc cgacgaagat cgttggaacg ctgttctcaa gctgcagact
     8401 ctgccgcgtg attccagccc gtctcgtcag cgtaaccgct gccgtcaaac aggtcgtccg
     8461 catggtttcc tgcggaagtt cgggttgagc cgtattaagg tccgtgaagc cgctatgcgc
     8521 ggtgaaatcc cgggtctgaa aaaggctagc tggtaattgt caccaattga atcacgggag
     8581 taaagacaga tgagcatgca agatccgatc gcggatatgc tgacccgtat ccgtaacggt
     8641 caggccgcga acaaagctgc ggtcaccatg ccttcctcca agctgaaagt ggcaatcgcc
     8701 aacgtgctga aggaagaagg ttttattgaa gattttaaag ttgaaggcga caccaagcct
     8761 gaactggaac ttactctgaa gtatttccag ggcaaagctg ttgtagaaag cattcagcgt
     8821 gtcagccgcc caggtctgcg catctataaa cgtaaagatg agctgccgaa agttatggcg
     8881 ggtctgggta tcgcagttgt ttctacctct aaaggtgtta tgactgatcg tgcagcgcgc
     8941 caggctggtc ttggtggcga aattatctgc tacgtagcct aatcggagga aaaaatgtct
     9001 cgtgttgcta aagcaccggt cgttgttcct gccggcgttg acgtaaaaat caacggtcag
     9061 gttattacga tcaaaggtaa aaacggcgag ctgacccgta ctctcaacga tgctgttgaa
     9121 gttaaacatg cagataatac cctgaccttc ggtccgcgtg atggttacgc agacggttgg
     9181 gcacaggctg gtaccgcgcg tgccctgctg aactcaatgg ttatcggtgt taccgaaggc
     9241 ttcactaaga agctgcagct ggttggtgta ggttaccgtg cagcggttaa aggcaatgtg
     9301 attaacctgt ctctgggttt ctctcatcct gttgaccatc agctgcctgc gggtatcact
     9361 gctgaatgtc cgactcagac tgaaatcgtg ctgaaaggcg ctgataagca ggtgatcggc
     9421 caggttgcag cggatctgcg cgcctaccgt cgtcctgagc cttacaaagg caagggtgtt
     9481 cgttacgccg acgaagtcgt gcgtaccaaa gaggctaaga agaagtaagg taacactatg
     9541 gataagaaat ctgctcgtat ccgtcgtgcg acccgcgcac gccgcaagct ccaggagctg
     9601 ggcgcaactc gcctggtggt acatcgtacc ccgcgtcaca tttacgcaca ggtaattgca
     9661 ccgaacggtt ctgaagttct ggtagctgct tctactgtag aaaaagctat cgctgaacaa
     9721 ctgaagtaca ccggtaacaa agacgcggct gcagctgtgg gtaaagctgt cgctgaacgc
     9781 gctctggaaa aaggcatcaa agatgtatcc tttgaccgtt ccgggttcca atatcatggt
     9841 cgtgtccagg cactggcaga tgctgcccgt gaagctggcc ttcagttcta aggtagaggt
     9901 gtaagatggc tcacatcgaa aaacaagctg gcgaactgca ggaaaagctg atcgcggtaa
     9961 accgcgtatc taaaaccgtt aaaggtggtc gtattttctc cttcacagct ctgactgtag
    10021 ttggcgatgg taacggtcgc gttggttttg gttacggtaa agcgcgtgaa gttccagcag
    10081 cgatccagaa agcgatggaa aaagcccgtc gcaatatgat taacgtcgcg ctgaataacg
    10141 gcactctaca acaccctgtt aaaggtgttc acacgggttc tcgcgtattc atgcagccgg
    10201 cttccgaagg taccggtatc atcgccggtg gtgcaatgcg cgccgttctg gaagtcgctg
    10261 gggttcataa cgttctggct aaagcatatg gttccaccaa cccgatcaac gtggttcgtg
    10321 caactattga tggcctggaa aatatgaatt ctccagaaat ggtcgctgcc aagcgtggta
    10381 aatccgttga agaaattctg gggaaataaa ccatggcaaa gactattaaa attactcaaa
    10441 cccgcagtgc aatcggtcgt ctgccgaaac acaaggcaac gctgcttggc ttgggtctgc
    10501 gtcgtattgg tcacaccgta gagcgcgagg atactcctgc tattcgcggt atgatcaacg
    10561 cggtttcctt catggttaaa gttgaggagt aagagatgcg tttaaatact ctgtctccgg
    10621 ccgaaggctc caaaaaggcg ggtaaacgcc tggggcgtgg tatcggttct ggcctcggta
    10681 aaaccggtgg tcgtggtcac aaaggtcaga agtctcgttc tggcggtggc gtacgtcgcg
    10741 gtttcgaggg tggtcagatg cctctgtacc gtcgtctgcc gaaattcggc ttcacttctc
    10801 gtaaagcagc gattacagcc gaagttcgtc tatctgacct ggctaaagta gaaggcggtg
    10861 ttgtagacct gaacacgctg aaagcggcta acattatcgg tatccagatc gagttcgcga
    10921 aagtgatcct ggctggcgaa gtaacgactc cggtaactgt tcgtggcctg cgtgttacta
    10981 aaggcgctcg tgctgctatc gaagctgctg gcggtaaaat cgaggaataa gtagcagatg
    11041 gctaaacaac cgggattaga ttttcaaagt gccaaaggtg gcttaggcga gctgaaacgc
    11101 agactgctgt ttgttatcgg tgcgctgatt gtgttccgta ttggctcttt tattccgatc
    11161 cctggtattg atgccgctgt acttgccaaa ctgcttgagc aacagcgagg caccatcatt
    11221 gagatgttta acatgttctc tggtggtgct ctcagccgtg cttctatctt tgctctgggg
    11281 atcatgccgt atatttcggc gtcgatcatt atccagctgc tgacggtggt tcacccaacg
    11341 ttggcagaaa ttaagaaaga aggggagtct ggtcgtcgta agatcagcca gtacacccgt
    11401 tacggtactc tggtgctggc aatattccag tcgatcggta ttgctaccgg tctgccgaat
    11461 atgcctggta tgcaaggcct ggtgattaac ccgggctttg cattctactt caccgctgtt
    11521 gtaagtctgg tcacaggaac gatgttcctg atgtggttgg gcgaacagat cactgaacga
    11581 ggtatcggca acggtatttc aatcattatc ttcgccggta ttgtcgcggg actcccgcca
    11641 gccattgccc atactatcga gcaagcgcgt caaggtgacc tgcacttcct cgtgttgctg
    11701 ttggttgcag tattagtatt tgcagtgacg ttctttgttg tgtttgttga gcgtggtcaa
    11761 cgccgcattg tggtaaacta cgcgaaacgt cagcaaggtc gtcgtgtcta tgctgcacag
    11821 agcacacatt taccgctgaa agtgaatatg gcgggggtaa tcccggcaat cttcgcttcc
    11881 agtattattc tgttcccggc gaccatcgcg tcatggttcg ggggcggtac tggttggaac
    11941 tggctgacaa caatttcgct gtatttgcag cctgggcaac cgctttatgt gttactctat
    12001 gcgtctgcaa tcatcttctt ctgtttcttc tacacggcgt tggttttcaa cccgcgtgaa
    12061 acagcagata acctgaagaa gtccggtgca tttgtaccag gaattcgtcc gggagagcaa
    12121 acggcgaagt atatcgataa agtaatgacc cgcctgacct tggttggtgc gctgtatatt
    12181 acctttatct gcctgatccc ggagttcatg cgtgatgcaa tgaaagtacc gttctacttc
    12241 ggtgggacct cactgcttat cgttgttgtc gtgattatgg actttatggc tcaagtgcaa
    12301 actctgatga tgtccagtca gtatgagtct gcattgaaga aggcgaacct gaaaggctac
    12361 ggccgataat tggtcgcccg agaagttacg gagagtaaaa atgaaagttc gtgcttccgt
    12421 caagaaatta tgccgtaact gcaaaatcgt taagcgtgat ggtgtcatcc gtgtgatttg
    12481 cagtgccgag ccgaagcata aacagcgcca aggctgattt tttcgcatat ttttcttgca
    12541 aagttgggtt gagctggcta gattagccag ccaatctttt gtatgtctgt gcgtttccat
    12601 ttgagtatcc tgaaaacggg cttttcagca tggaacgtac atattaaata gtaggagtgc
    12661 atagtggccc gtatagcagg cattaacatt cctgatcata agcatgccgt aatcgcatta
    12721 acttcgattt atggcgtcgg caagacccgt tctaaagcca tcctggctgc agcgggtatc
    12781 gctgaagatg ttaagatcag tgagctgtct gaaggacaaa tcgacacgct gcgtgacgaa
    12841 gttgccaaat ttgtcgttga aggtgatctg cgccgtgaaa tcagcatgag catcaagcgc
    12901 ctgatggatc ttggttgcta tcgcggtttg cgtcatcgtc gtggtctccc ggttcgcggt
    12961 cagcgtacca agaccaacgc acgtacccgt aagggtccgc gcaaaccgat caagaaataa
    13021 tcggggtgat tgaataatgg caaaggcacc aattcgtgca cgtaaacgtg taagaaaaca
    13081 agtctctgac ggcgtggctc atatccatgc ttctttcaac aacaccatcg tgactatcac
    13141 tgatcgtcag ggtaacgcgt tgggttgggc aacagccggt ggttccggtt tccgtggttc
    13201 tcgcaaatcc actccgtttg cagctcaggt tgcagcagag cgttgcgctg acgccgtgaa
    13261 agaatacggc atcaagaatc tggaagttat ggttaaaggt ccgggtccag gccgcgaatc
    13321 tactattcgt gctctgaacg ccgcaggttt ccgcatcact aacattactg atgtgactcc
    13381 gatccctcat aacggttgtc gtccgccgaa aaaacgtcgc gtataacgcc tcgttttcca
    13441 ggtttgttgg agaaagaaaa tggcaagata tttgggtcct aagctcaagc tgagccgtcg
    13501 tgagggcacc gacttattcc ttaagtctgg cgttcgcgcg atcgatacca agtgtaaaat
    13561 tgaacaagct cctggccagc acggtgcgcg taaaccgcgt ctgtctgact atggtgtgca
    13621 gttgcgtgaa aagcaaaaag ttcgccgtat ctatggtgtg ctggagcgtc agttccgtaa
    13681 ctactacaaa gaagcagcac gtctgaaagg caacaccggt gaaaacctgt tggctctgct
    13741 ggaaggtcgt ctggacaacg ttgtataccg tatgggcttc ggtgccactc gtgcagaagc
    13801 acgtcagctg gttagccata aagcaattat ggtaaacggt cgtgttgtta acatcgcttc
    13861 ttatcaggtt agtccgaatg acgttgtaag cattcgtgag aaagcgaaga agcagtctcg
    13921 cgtgaaagcc gctctggagc tggctgagca gcgtgaaaag ccaacttggc tggaagttga
    13981 tgctggcaag atggaaggta cgtttaagcg taagccggag cgttctgatc tgtctgcgga
    14041 cattaacgaa cacctgatcg tcgagcttta ctccaagtaa agcttagtac caaagagagg
    14101 acacaatgca gggttctgtg acagagtttc taaaaccgcg cctggttgat atcgagcaag
    14161 tgagttcgac gcacgccaag gtgacccttg agcctttaga gcgtggcttt ggccatactc
    14221 tgggtaacgc actgcgccgt attctgctct catcgatgcc gggttgcgcg gtgaccgagg
    14281 ttgagattga tggtgtacta catgagtaca gcaccaaaga aggcgttcag gaagatatcc
    14341 tggaaatcct gctcaacctg aaagggctgg cggtgagagt tcagggcaaa gatgaagtta
    14401 ttcttacctt gaataaatct ggcattggcc ctgtgactgc agccgatatc acccacgacg
    14461 gtgatgtcga aatcgtcaag ccgcagcacg tgatctgcca cctgaccgat gagaacgcgt
    14521 ctattagcat gcgtatcaaa gttcagcgcg gtcgtggtta tgtgccggct tctacccgaa
    14581 ttcattcgga agaagatgag cgcccaatcg gccgtctgct ggtcgacgca tgctacagcc
    14641 ctgtggagcg tattgcctac aatgttgaag cagcgcgtgt agaacagcgt accgacctgg
    14701 acaagctggt catcgaaatg gaaaccaacg gcacaatcga tcctgaagag gcgattcgtc
    14761 gtgcggcaac cattctggct gaacaactgg aagctttcgt tgacttacgt gatgtacgtc
    14821 agcctgaagt gaaagaagag aaaccagagt tcgatccgat cctgctgcgc cctgttgacg
    14881 atctggaatt gactgtccgc tctgctaact gccttaaagc agaagctatc cactatatcg
    14941 gtgatctggt acagcgtacc gaggttgagc tccttaaaac gcctaacctt ggtaaaaaat
    15001 ctcttactga gattaaagac gtgctggctt cccgtggact gtctctgggc atgcgcttgg
    15061 aaaactggcc accggcaagc atcgctgacg agtaaccgga tcacaggtta aggttttact
    15121 gagaaggata aggtcatgcg ccatcgtaag agtggtcgtc aactgaaccg caacagcagc
    15181 catcgccagg ctatgttccg caatatggca ggttcactgg ttcgtcatga aatcatcaag
    15241 acgactctgc ctaaagcgaa agagctgcgc cgcgtagttg agccgctgat tactcttgcc
    15301 aagactgata gcgttgctaa tcgtcgtctg gcattcgccc gtactcgtga taacgagatc
    15361 gtggcaaaac tgtttaacga actgggcccg cgtttcgcga gccgtgccgg tggttacact
    15421 cgtattctga agtgtggctt ccgtgcaggc gacaacgcgc cgatggctta catcgagctg
    15481 gttgatcgtt cagagaaagc agaagctgct gcagagtaat ctgaagcaac gtaaaaaaac
    15541 ccgccccggc gggttttttt atacccgtag tatccccact tatctacaat agctgtactc
    15601 tttt
//
LOCUS       DADMGC010000093        15597 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.093, whole genome shotgun sequence.
ACCESSION   DADMGC010000093 DADMGC010000000
VERSION     DADMGC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.093"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            112..1170
                     /gene="citC"
                     /locus_tag="JNZ73_002870"
     CDS             112..1170
                     /gene="citC"
                     /locus_tag="JNZ73_002870"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAY8733093.1"
                     /translation="MFVNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKLRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWFHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1185..1481
                     /gene="citD"
                     /locus_tag="JNZ73_002871"
     CDS             1185..1481
                     /gene="citD"
                     /locus_tag="JNZ73_002871"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAY8733094.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1478..2386
                     /gene="citE"
                     /locus_tag="JNZ73_002872"
     CDS             1478..2386
                     /gene="citE"
                     /locus_tag="JNZ73_002872"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAY8733095.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACVREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2397..3929
                     /gene="citF"
                     /locus_tag="JNZ73_002873"
     CDS             2397..3929
                     /gene="citF"
                     /locus_tag="JNZ73_002873"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAY8733096.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3933..4484
                     /gene="citX"
                     /locus_tag="JNZ73_002874"
     CDS             3933..4484
                     /gene="citX"
                     /locus_tag="JNZ73_002874"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAY8733097.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4459..5337
                     /gene="citG"
                     /locus_tag="JNZ73_002875"
     CDS             4459..5337
                     /gene="citG"
                     /locus_tag="JNZ73_002875"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAY8733098.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5388..6851
                     /gene="citT"
                     /locus_tag="JNZ73_002876"
     CDS             5388..6851
                     /gene="citT"
                     /locus_tag="JNZ73_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HAY8733099.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMDKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNML
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            6965..7771
                     /gene="rna"
                     /locus_tag="JNZ73_002877"
     CDS             6965..7771
                     /gene="rna"
                     /locus_tag="JNZ73_002877"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HAY8733100.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8000..8410
                     /gene="rnk"
                     /locus_tag="JNZ73_002878"
     CDS             8000..8410
                     /gene="rnk"
                     /locus_tag="JNZ73_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HAY8733101.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLRDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8641..9878)
                     /locus_tag="JNZ73_002879"
                     /pseudo
     CDS             complement(8641..9878)
                     /locus_tag="JNZ73_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            10099..10527
                     /gene="uspG"
                     /locus_tag="JNZ73_002880"
     CDS             10099..10527
                     /gene="uspG"
                     /locus_tag="JNZ73_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HAY8733102.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10648..12213)
                     /gene="ahpF"
                     /locus_tag="JNZ73_002881"
     CDS             complement(10648..12213)
                     /gene="ahpF"
                     /locus_tag="JNZ73_002881"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAY8733103.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12458..13021)
                     /gene="ahpC"
                     /locus_tag="JNZ73_002882"
     CDS             complement(12458..13021)
                     /gene="ahpC"
                     /locus_tag="JNZ73_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HAY8733104.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13393..14139
                     /gene="dsbG"
                     /locus_tag="JNZ73_002883"
     CDS             13393..14139
                     /gene="dsbG"
                     /locus_tag="JNZ73_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HAY8733105.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKHGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14349..15251
                     /locus_tag="JNZ73_002884"
     CDS             14349..15251
                     /locus_tag="JNZ73_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8733106.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15398..15597
                     /locus_tag="JNZ73_002885"
                     /pseudo
     CDS             15398..15597
                     /locus_tag="JNZ73_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3822 a         4059 c         3808 g         3908 t
ORIGIN      
        1 ctcgatcacc cccctttctc ccaagtgaaa ataaaaggtt atcagtttgc aacattgaac
       61 aacattcgtt gcaaatcgat aacaacatgc accttcagga tactatttat tatgttcgtc
      121 aatgatattt tcacccgcgt aaaacgttca gaaaataaaa aaatggcgga aatcgcccaa
      181 ttcctgcatg aaaatgattt gagcgttgac accacagtcg aagtatttat taccgtaacc
      241 cgcgatgaaa agcttatcgc gtgcggtgga attgccggaa atattattaa atgcgttgct
      301 atcagtgaat ccgttcgcgg tgaaggactg gcgctgacat tagccactga actgataaac
      361 ctcgcctatg agcggcacag cacgcatctg tttatttata ccaaaaccga atacgaggcg
      421 ctgttccgcc agtgcggttt ttccacgctg acctgcgtac ccggcgtgat ggtgctgatg
      481 gaaaacagcg ccacgcgact gaaacgctat gccgaatcgc tgaaaaaatt gcgtcatcca
      541 gggaacaaga ttggctgcat tgtgatgaac gccaatccct ttacgaatgg tcaccgttat
      601 ctgattcaac aagctgcggc acagtgcgac tggtttcatc tgtttttagt caaagaagat
      661 tcttcacgct tcccctatga agaccggctg gatctggtgt taaaaggcac cgccgatatt
      721 ccacgcctga ctgtgcatcg cggctcagaa tacatcatct cccgcgctac gttcccttgc
      781 tacttcatta aagaacagag cgtcattaac cattgttaca ccgaaattga tctgaagatt
      841 ttccgtcagt acctcgctcc cgcgcttggt gtaactcacc gctttgtcgg tactgaaccc
      901 ttttgtcgcg ttaccgccca gtacaaccag gatatgcgct actggctgga aacgccgact
      961 atctccgcag cgcccatcga actggttgaa attgagcggc tgcgttacca ggagatgccg
     1021 atatccgctt cccgggtacg tcaactgctg gcgaaaaacg atctcacggc tatcgcgccg
     1081 ctggtccctg cagtcacgct gcattatttg cagaacctgc ttgaacactc ccgccaggac
     1141 gcggcagctc gtcaaaagac ccccgcatga gaaacaggtg aaaaatgaaa ataaaccagc
     1201 ccgccgttgc aggcaccctt gagtctgggg atgtgatgat acgcatcgcc ccactcgata
     1261 cgcaggatat cgacctgcaa atcaatagca gcgttgagaa acagtttggc gatgcaattc
     1321 gcaccaccat tctggacgtt ctcgcccgct acaacgtgcg cggcgtacag ctgaatgtcg
     1381 atgacaaagg cgcactggac tgcattttac gtgcacgact ggaagccctg ctggcacgcg
     1441 ccagcggtat cccggctctg ccatgggagg attgccaatg atttccgctt cgctgcaaca
     1501 acgtaaaact cgcacccgcc gcagcatgtt gtttgtgcct ggtgccaatg ccgcgatggt
     1561 cagcaactcc ttcatctacc cggctgatgc cctgatgttt gacctcgaag actccgtggc
     1621 attacgtgaa aaagacaccg cccgccgcat ggtttatcac gcgctgcaac atccgctgta
     1681 tcgcgatatt gaaaccattg tgcgtgtcaa cgcgctggat tccgaatggg gtgttaacga
     1741 cctggaagcc gtcgttcgcg gtggtgcgga cgttgtgcgt ctgccgaaaa ccgataccgc
     1801 tcaggatgtt ctggatattg aaaaagagat cctgcgtata gaaaaagcct gtgttcgtga
     1861 acccggcagc accggcctgc tggcggcgat tgaatctccg ctgggcatta cccgcgcagt
     1921 ggaaatcgct cacgcttccg agcgtttgat cggtatcgcc ctcggtgcag aagactatgt
     1981 gcgcaacctg cgtacagaac gctccccgga aggaactgaa ctgctgttcg cacgctgttc
     2041 cattttgcag gccgcgcgct ctgcgggtat tcaggcgttc gataccgtct attccgacgc
     2101 taacaacgaa gccggattcc tgcaagaagc cgcccacatc aaacagctgg gctttgacgg
     2161 caaatcgctg atcaacccgc gtcagattga tctgctgcac aacctctacg caccgaccca
     2221 gaaagaagtg gatcacgccc gccgcgtcgt agaagccgct gaagccgccg ctcgcgaagg
     2281 cctcggcgtg gtttccctga acggtaagat ggtggacggt ccggttatcg atcgcgcccg
     2341 tctggtgctc tcccgtgcag aactttccgg catccgcgaa gaataaggca atcaaaatga
     2401 cgcagaaaat tgaacaatct caacgacaag aacgggtagc ggcctggaat cgtcgcactg
     2461 aatgcgatct tgccgctttc cagaactcac caaagcaaac ctaccaggct gaaaaagcgc
     2521 gcgatcgcaa actgtgcgcc aacctggaag aagcgattcg tcgctcaggt ttacaggacg
     2581 gcatgacggt ttccttccat cacgctttcc gtggcggtga cctgaccgtc aatatggtga
     2641 tggacgtcat cgcgaagatg ggctttaaaa acctgaccct ggcgtccagc tccctgagtg
     2701 attgccatgc gccgctggta gaacacattc gccagggcgt ggttacccgc atttatacct
     2761 ctggcctacg cggtccactg gcggaagaga tctcccgtgg tctgctggcg gaaccggtgc
     2821 agatccactc tcacggcggt cgtgtgcatc tggtacagag cggcgaactg aatatcgacg
     2881 tggctttcct cggcgtcccg tcctgtgatg aattcggtaa tgccaacggc tacaccggta
     2941 aagcctgctg cggctccctc ggctatgcaa tggttgatgc cgacaacgca aaacaggtcg
     3001 tgatgcttac cgaagaactg ctgccttatc cgcataatcc ggcaagcatt gagcaagatc
     3061 aggttgattt gatcgtcaaa gttgatcgcg ttggcgatgc tgcaaaaatc ggcgctggta
     3121 ctacccgtat gaccactaac ccgcgcgaac tgcttattgc ccgtagcgct gcggatgtga
     3181 ttgtcaactc tggctacttc aaagaaggtt tctccatgca aaccggcacc ggcggcgctt
     3241 cgctggcggt aacccgtttc ctggaagaca aaatgcgtag ccgcgatatt cgcgccgact
     3301 tcgccctcgg cggtattacc gcgacgatgg ttgatctgca cgaaaaaggt ctgatccgca
     3361 aactgctgga tgtgcagagc tttgacagcc atgctgcgca atcgctggcc cgtaacccca
     3421 atcacatcga aatcagcgcc aaccagtacg ctaactgggg ttcgaaaggc gcatcggttg
     3481 atcgtctcga cgtggtggta ctgagcgcgc tggaaattga caggcagttc aacgttaacg
     3541 tgctgaccgg ttctgacggc gtactgcgtg gcgcttccgg tggtcactgc gataccgcga
     3601 ttgcctctgc gctttccatc atcgtcgcgc cactggtacg cggtcgtatt ccgactctgg
     3661 tggataacgt gctgacctgc atcaccccgg gctccagtgt cgatattctg gtcacagacc
     3721 acggtatcgc agttaacccg gcacgtccgg aactagcaga acgtctgcag gaagcgggca
     3781 ttaaagtggt ttccattgag tggctgcgcg aacgtgcgcg tctgctgacc ggtgaaccac
     3841 agccgattga attcaccgac cgcgtcgttg ccgttgtgcg ttaccgcgat ggctcggtga
     3901 tcgatgttgt gcatcaggtg aaggaataag ccatgcacct gcttcctgaa ctcgccagcc
     3961 accatgcggt gtcaattccc gagctgctcg tcagccggga tgaaaggcaa gcacggcaac
     4021 acgcctggct caagcaccat cctgttccac tggtctcctt taccgtggtt gcgcctgggc
     4081 cgattaaaga cagcgaggtc acacgccgaa tttttaatca tggcgtgaca gccttgcgtg
     4141 ccttagccac aaagcagggc tggcaaattc aggagcaggc tgcactggtt tccgccagcg
     4201 ggccggaggg catgttgagc attgccgccc cggctcgcga tctcaagctc gccaccattg
     4261 agcttgaaca tagtcatcct ctcgggcggt tatgggatat cgatgtcctg acgcccgaag
     4321 gcgaaattct ctcccgccgc gactattcac tgccgcctcg ccgctgcctg ttgtgcgagc
     4381 aaagcgcagc cgtctgcgcg cgtggaaaaa cccatcaact gaccgattta ctcaaccgca
     4441 tggaggcact gctgaacgat gtcgatgcct gcaacgtcaa ctaaaaccac aaagcttgcg
     4501 acgtcattaa tcgatgagta cgccctgctg ggctggcgcg ccatgctgac tgaagtcaat
     4561 ctgtcaccga aaccaggcct cgtggatcgc attaactgcg gtgcgcacaa agatatggcg
     4621 ctggaagatt tccaccgcag cgcgctggcg attcagggct ggttaccccg tttcattgaa
     4681 tttggtgcct gtagtgcaga aatggcaccg gaagcggtac tccacggatt acgcccaatt
     4741 ggtatggctt gcgaaggtga tatgttccgc gccactgcgg gcgtaaacac gcataaaggc
     4801 agcatttttt ctttagggct gctatgtgcg gcaattggcc gtttgcttca gctcaaccag
     4861 ccggttacgc caacaaccgt ttgttctacg gcggcaagtt tctgccgtgg cctgaccgat
     4921 cgcgaactgc gtaccaataa ttcacgactg acggcaggtc aacggttgta ccagcagctt
     4981 ggccttaccg gcgcacgcgg tgaagccgaa gcgggttatc cactggtgat caaccacgcc
     5041 ttgccgcatt acctcactct gctggatcag gggttagatc ctgaactggc attgctcgat
     5101 accttgctcc tactgatggc taccaacggc gataccaacg ttgcatcgcg cggtggcgag
     5161 gggggcctgc gctggctaca gcgcgaggcg caaacattat tgcaaaaagg gggcattcga
     5221 acccccgccg atctcgatta tctccggcag ttcgacaggg agtgtatcga acgaaatctc
     5281 agtccaggcg gcagtgccga cctgctgatc cttacctggt ttttagcaca gatttaatta
     5341 tttaagcact tgataaattt ggaaatatta attttcggag aacccgtatg tctttagcaa
     5401 aagataatat atggaaacta ttggccccac tggtggtgat gggtgtcatg tttcttatcc
     5461 ctgtccccga cggtatgccg ccgcaggcat ggcattactt cgctgtgttt gtggcaatga
     5521 ttgtcggcat gatcctcgag ccaattccgg caacagcgat cagttttatt gcggttacta
     5581 tttgcgttat tggcagtaat tacctgctct ttgatgccaa agaattagct gacccagcgt
     5641 ttaatgcgca aaaacaggcg ctgaaatggg gcctggctgg tttttccagc accacggtat
     5701 ggctggtatt tggcgcattt atttttgcat tagggtatga agtttccggg ttaggtcgtc
     5761 gcattgccct tttcctggtg aaattcatgg acaaacgcac gctgacgttg ggttatgcga
     5821 ttgtcattat cgacattctg ctggcaccgt ttacaccgtc caacaccgcg cgtaccgggg
     5881 gtacggtttt tccggtcatt aaaaacctgc cgccgttgtt taaatcattc ccgaacgatc
     5941 cgtccgcgcg tcgtattggc ggctatttga tgtggatgat ggtcattagt accagtctga
     6001 gttcatccat gtttgtcacc ggtgcggcac caaacatgct gggtctggag ttcgtcagca
     6061 aaattgccgg tatccagatt agctggttgc agtggttcct ctgcttcctg ccggttgggg
     6121 ttatcttgct tatcattgcg ccgtggcttt cctacgtgct gtacaaaccg gaaatcacac
     6181 acagtgaaga agtggcaacc tgggcgggtg atgaactaaa aaccatgggt gcgctgacac
     6241 gcagagagtg gacgctgatt ggccttgtat tgctcagctt aggtttgtgg gtatttggca
     6301 gtgaagtcat taatgctact gcggttggtc tgctggcagt ttcgctaatg ctggctctgc
     6361 acgttgtgcc gtggaaagac attacccgct ataacagcgc atggaacacg ctggtcaacc
     6421 tggcaactct ggttgtgatg gctaacggcc tgactcgttc tggttttatt gactggttcg
     6481 ccggtaccat gagtacgcac ctggaaggat tctcaccaaa cgcaacggtg attgtactgg
     6541 ttctggtgtt ctactttgca cactacctgt ttgccagcct gtctgcgcac accgcaacca
     6601 tgctgccggt tattctggcc gtcggtaaag gtattccggg cgtaccaatg gaacaactgt
     6661 gtatcctgct ggtgctgtct atcggtatca tgggctgtct gacgccgtat gcaaccggtc
     6721 ctggggtgat tatttacggc tgtggctatg tgaaatcaaa agattactgg cgtcttggcg
     6781 caatcttcgg ggtgatttac atctctatgt tactgttggt tggctggccg attctcgcca
     6841 tgtggaacta atacgttcgc caaacgctga ataaatcatc acgcccgcca ggtaagccca
     6901 cctggcgggc ttttttatga tttaatagat agtattcagt gtatgtatga gttccacacc
     6961 cattatgaaa gcattctgcc gtaacgccgc gttgctcgcg gtttctctgc ttcccttatc
     7021 ttctgccaac gccgtagcgt tgcaggcaaa acagtatggc gattttgatc gctatgtcct
     7081 ggccctctcc tggcaaaccg gattttgcca gagtcaacac gatcgaaatc gtaacgaacg
     7141 agatgaatgt cgcctgcaaa ccgaaacgac caacaaagct gattttctga ccgtacatgg
     7201 tctgtggcca ggattgccta aatcggttgc tgcccgtggt gttgatgaac gccgctggat
     7261 gcgcttcggt tgcgctactc gcccaatccc gaatctacca gaagcacgcg ccagccgaat
     7321 gtgttcatcg ccggaaaccg ggttatcact ggaaacggcc gctaaactaa gtgaagtcat
     7381 gccaggagct ggcggacgtt cctgcctgga acgctacgaa tatgccaaac acggtgcctg
     7441 ctttggtttt gatccggacg catacttcgg tacgatggta cgcctgaatc aagaaattaa
     7501 agagagcgaa gccggaaaat tccttgcgga taattacggt aaaacagtga gccgccgtga
     7561 ctttgacgcc gcctttgcca aaagctgggg aaaagagaac gtgaaagcag ttaagctaac
     7621 gtgccagggt aaccctgcgt atttgactga aattcagatc tcgatcaaag ctgacgccat
     7681 caacgctccg ctttctgcaa actcattctt gccacaacct cacccaggta actgtggcaa
     7741 aacctttgtg attgataaag cgggttatta atcctctgat ttacaaaaca cgctaattcg
     7801 tcttccataa ccatgccgat taagaattat acataatgcg ctgcattgag gaattccctg
     7861 aatgtgacgc aaatcacttc aaagagtgag caacagtcag tatcatcatg tgagttaaac
     7921 cctcgccgcc tgacggtgag ggttttcttt tgggtatgtt tatctgcgac actcgcagta
     7981 ccgacgacat ggagtaaaaa tgtccagacc aactatcatc attaacgacc tggatgccga
     8041 acgcatcgat attctgctgg agcaacccgc ctatgctggt ttgccaatcg ccgacgcgtt
     8101 aaacgcagag ttggatcgcg cccaaatgtg ttcgccagaa gagatgccac acgacgtggt
     8161 gacaatgaac agccgggtta aattccgcaa tcttagagat ggcgaagtgc gtgtgcgcac
     8221 gctggtgtat ccggcaaaaa tgaccgatag caatactcag ctttccgtta tggctccggt
     8281 aggtgccgca ctgctggggc tgcgcgttgg cgattccatt cactgggaac ttccgggcgg
     8341 cgttgcaacc caccttgaag tgctggaact cgaataccag ccagaagctg ctggcgacta
     8401 cctgctttaa tcccatctgc acaagcgata actcgctttg cccagaggat gcactcgcat
     8461 cctctttacg caaaatcagc aatttttcct ggttctcact gttcactttt gctgcaaaat
     8521 tattccgttt cgtttgtggg aataaccatg gcagaagcat tttacatcct gataggattt
     8581 ctgattatgg cggcgattat cgtcatggct gtcctttacc tcgaaaacca ctcctgacga
     8641 tcatattgtt ccccccggca tcgcattcac cagacctgaa accgccttct gcgccgcctc
     8701 tgcgctttgt gcaccgggta ccagaatcac cttacggcac tcctcttcac gtttttcgaa
     8761 aatctcatat ccccgggcgg cctcttcaaa cggcatatag tgggtaacaa tttcttctgg
     8821 tttcagtaat cctttctcaa ttaacggtaa taattctccc agccatgcgt gaacgtgggt
     8881 ctgtcccatt ttaaacgtca accctttatc aaaggcgtcg ccaaacagga aaccgtgaat
     8941 aaatccagcg tagacgcccg gtacgctaac aatgccgcca cgcctgaccg ccgcaataca
     9001 ctggcgcaac gctttaccgc tgctgccctc cagtttcagg ttagtcagca ccgtttccgt
     9061 ggtgctgcct ttcgcttcaa aaccgacggc gtctattact gcatccacgc cccggtgacc
     9121 tgccgtttgt tcaataattg actgtgccgg atcgctatct tcatcgaaat taatcgggat
     9181 cgcgccgtag cggtcagcgg cgaaacgcaa gcggtaggga tgatgatcaa caacaaaaat
     9241 ctgttccgca ccgagcaacc gtgcacaggc gattgtcaac aatcccacag gaccagcacc
     9301 atagactgca acgcttgaac cttgttggat ctgcacattt tttgctgcct gccatgccgt
     9361 tggcagaata tcagaaagga aaagggcttt atcatctgaa agcaaaggcg gtactttaaa
     9421 cggccccaca ttccctttag ggacgcggac atattccgcc tgcccgctag gaacgccacc
     9481 atacaggtga ctataaccaa acaatgccgc gggagcaggt atctgttttt tattgagcgc
     9541 agcgccttta cccgcattgg tattttcgca ggcggcatat tgctgtaatc gacagaaaaa
     9601 acagtcgcca caagcaatga cgaacggaat taccacccgg tcgccttttt gcaaattttt
     9661 tacgtccttc ccggtttcaa cgacttcccc ataaattcat gaccaaaaat atcgccatgt
     9721 ttaacctggg gtattttgcc tcgataaaga tggaggtcag agccacagat cgccgttacc
     9781 gtaatacgca gaataatatc atctgcctgt tcaatgcccg gatcgggaac attttctacc
     9841 tgaacgtgat gtgggccgtg ataagtcaat gctttcatgc aacctccaga gacagttcaa
     9901 aaaagagtca gtctttaagg gtagctatca cagcgggaaa acgggcggta ccgcaggttt
     9961 tgggagaatt cctggtgttc agtgagaata tattaatcac agaaaccgtt cgtaaggaaa
    10021 aagaaccaga caaatatcag ggataaatat tataatcatg gcgaatgatt tgttcatgat
    10081 taacagggag aaagggttat gtataaggca atcattatgc cagttgatgt atttgaaatg
    10141 gaattgagcg acaaagctgt tcgccacgct gaattcctcg cccaggatga cggagttatt
    10201 catctacttc acgtactacc cgggtcagcc agcctgagcc tgcaccgttt tgccgctgat
    10261 gtgcgtcgtt ttgaagagca tctgcaacat gaagcagaag aacgtctgca aacgatggtc
    10321 agccacttca ccatcgatcc ttcccgcatt aaacaacatg tccgttttgg tagcgtgcgg
    10381 gatgaagtca atgagttggc agaagaactg ggggctgatg ttgtagttat cggttctcgc
    10441 aacccatcga tttcgaccca tctgttaggt tctaacgcct cgagcgtgat ccgccacgcc
    10501 aatctgcctg tactggtcgt tcgctaacag ataaaagtat ttatgccgcc ctgcctggaa
    10561 catcccggca gggcttttta atgccataaa aaagccgcca ggtttgaccc cggcggctaa
    10621 gcaattgcag gtgaatctta cttcttctta tgcagttttg gtgcgaatca ggtagtcaaa
    10681 agcactcaga gaggctttgg caccttcgcc agtggcgatg atgatctgct tgtacggaac
    10741 cgtcgtacag tcacccgctg cgaacacgcc tttgacgttg gtttcacatt tcgcatcaat
    10801 gataatctcg cccatgcggt tacgttcgac tgcgccttcc agccagttgg tgttcgggag
    10861 cagaccaatc tggacaaaaa taccggccag ttcgatgttg tgaatattgc cgctgacacg
    10921 atctcgatat tccagaccaa cgactttgct gccgtcgcct ttcacttccg tggtttgcgc
    10981 attcagaata atgtcgacgt ttttcaggct gcgcagtttg tcctgcagaa cctggtcggc
    11041 tttcatttct ggcgcaaatt ccagcagcgt tacgtgctca acgatacccg ccaggtcgat
    11101 tgccgcttcc acgccggagt taccgccgcc gataaccgct acgcgtttgc ctttaaacag
    11161 cgggccgtcg cagtgcgggc agtaggtcac gcctttagtg cgatactgat cttcgcccgg
    11221 aacgttcatg ttgcgccatt ttgcacccgt cgccacgata atgctgcgtg ctttcagtac
    11281 cgcgccagaa gctgtttcaa tctggtgtag accaccttca accgctgccg ggatcagttt
    11341 gctggcgctc tggctgtcga tcacatcaac gtcgtattca tcaacgtgaa ctttcagtgc
    11401 gcctgccaac ttctgccctt cagtcttcgg tacagaaatg tagttttcga tatcaacggt
    11461 atcgaggatc tgaccaccaa aacgttcgcc catcagaccg gtacggatgc ctttacgtgc
    11521 ggagtaaatt gctgccgctg cacccgccgg gccggaacca acgattaata cgtcataagc
    11581 atcacgcttg ttcagctctt ctgccgcacg tttttccgcg ccagtatcaa ttttggcaac
    11641 gatttcagtc aacgtcatgc ggccctgacc aaactctttc ccgtttacga acactgccgg
    11701 aacgcccatc acgttgcgat cggtgatttc gttctggaag gtgccgccgt caattgcagt
    11761 gtgcttgatg cgcgggttca atacgctcat caggttcagc gcctgcacca cgtccgggca
    11821 gttatggcaa gagagcgagt aataggtttc gaattcaaaa tcaccgtcaa tatggcgaat
    11881 ctgctccagc agagactgcg cttctttcga cggatgacca ccggtccaca gcaacgccag
    11941 taccagcgag gtgaactcgt ggcccagcgg ggagcctgca aaacgtggtc cctggttgga
    12001 acctgggttg gtgatcagga aagacggctt acgcactggc aagctgttat cttctttaaa
    12061 ggtgactttg tctgacagtt ctgcgatttc agccaacagt tccttgattt ctgccgattt
    12121 agcgctgtca tccagcgtgg caattaactc aacaggcttg gtcaatttct caaggtaagc
    12181 cttgagttga gttttcatat ttgtgtcgag catgtttatc tcctgggctt taaacatcat
    12241 catgcaagcg gcctctgcgg ctacctggat gcagcttgca tcatggtgcg gggaagaaaa
    12301 tcgggtgccg cagcacccga agaattaggt gaatttccga agtatttcgc gtcagagcaa
    12361 ggcgactagc ctgtgaatcc ccgggagctt acacaagtaa gtgaccgggg tgagcgggcg
    12421 acgccaacgc cgctatggcg tgaaagacga aggaaattta gattttacca accaggtcca
    12481 gagacggagc cagagttgct tcaccttctt tccatttagc cgggcaaact tcacctgggt
    12541 gagaagctac gtactgagct gctttgattt tacgcagcag atcagacgcg tcacggccaa
    12601 tgccttcagc ggtaacttcg attgcctgga tgataccctg cgggtcaaca acgaaggtcg
    12661 cacggtcagc cagaccttca tcttcacgca tgttgtcgaa gttacgggtc agggcgccag
    12721 tcgggtcgcc gatcatcgca tatttgattt tagcgatggt ttcagagctg ctgtgccatg
    12781 ctttgtgggt gaagtgagta tcggtagata ctgcgtatac gtctacgccc agtttctgca
    12841 gttcttcgta gtggtcagca acgtcaccca gttcggtcgg gcatacgaaa gtaaagtcag
    12901 ccgggtagaa gaagaagacg ctccagcggc cttcggtatc tttttcggtg atttcgatga
    12961 attcgccgtt tttgaatgcc tggtttttaa aaggtttaat tttggtgtta atcaaggaca
    13021 tctatacttc ctccgtgttt tcgatgagat gtaaggtaac caatttttgc cgattcggct
    13081 aatgcgtttc cattatcaaa tcgataagtt ttaccttaca accttcgtaa gacaactttg
    13141 tgaactttgc accgccagcg gcgataaaaa gtaaaatggc cccctttcca ggtgaccgtg
    13201 taatctgaac taccctttgg tatcttcagc gcctgcctgc aggctgaggt gttgccctta
    13261 caaatgcaac aacgacatgg attacaacac cctcataaac aaagggcaat cacctgatct
    13321 aagctcttac ctatgacagt gataggttat gccttttact cgacttttgc actgactgaa
    13381 aagaacaaat taatgttaaa aaagatactt ttactggctc tgcttcctgc aatcgccttc
    13441 gcagaggaac ttccttcccc agtaaaagcg attgaaaaac atggcattac aatcatcaaa
    13501 acgttcgatg cccccggagg aatgaaaggt tatctcggaa agtatcagga tatgggcgtc
    13561 accatctacc tgactcccga tggtaagcac gctatctctg gttacatgta caacgagaaa
    13621 ggtgaaaacc tgagtaacac acttatcgaa aaagaaattt acgcaccagc cggacgcgaa
    13681 atgtggcaac ggatggaaca atcccactgg ctcctcgacg gtaaaaaaga tgcgccggtc
    13741 attgtctacg tcttcgccga tccgttctgc ccatattgta aacagttctg gcagcaggcg
    13801 cgcccgtggg tagattctgg caaagtgcaa ttaagaacat tgttggttgg ggttatcaag
    13861 ccagaaagcc cggcgacagc agcggcaatt cttgcctcca aagatcccgc aaaaacctgg
    13921 caacaatatg aagcctctgg ttgcaagctt aagctaaacg tgcctgcaaa cgtaagtaca
    13981 gagcaaatga aagtgttaag tgacaatgag aaactgatgg acgatctggg ggcaaatgtc
    14041 acgccggcta tctattacat gagtaaggaa aatacgctgc aacaggccgt ggggttgccc
    14101 gatcagaaaa cgcttaatat cattatggga aataaataaa aggtaattca tggatggttg
    14161 aactatatcc ctgcttttca accatcctaa aaaagcatga attgctcgct ctaccctaag
    14221 tggctgtgct attttagaaa aacttaatcc tgagttaagc aaaaaaacat aatcaataaa
    14281 aatacatacg caaaaaacat tgattaagtg aatataccat ggaagaaaaa tataaaccgg
    14341 agtagtgtat ggccaatctc tacgacttga aaaagttcga cttaaatctt cttgtcatat
    14401 ttgagtgtat ttaccagcat ttaagcatta gcaaagctgc tgaatcacta tatatcaccc
    14461 catcagccgt gagccagtcg ttacaacgct tacgtgcgca atttaatgac cctttattca
    14521 ttcgctcagg aaaaggtatc gcgccgacga caacagggtt gaatctgcat caccatcttg
    14581 agaaaaacct caaaggcctt gaacaaacaa ttaatatagt caataagtcc gaactaaaga
    14641 aaaactttat tatctacggc cctcagctca tttcttgctc taataatagt atgctgatcc
    14701 gctgcctacg gcaagatgcc tctgtcgaga ttgagtgcca tgatatcctc atgtcagcag
    14761 agaatgctga agaactgttg gttcaccgca aagccgatct ggtgatcacc caaatgccag
    14821 tgatcagccg ttccgtcatt tgcatgccat tgcatacaat tcgcaatact ctaatttgta
    14881 gtaataagca tccgcgaatt actgacaata gcacttatga acaaattatg gcagaagagt
    14941 tcacccaatt aatatccaaa tcagcaggag ttgatgatat tcaaatggaa atagacgaga
    15001 agtttatgaa tcgcaaaatt tcatttcgtg gttcctcttt gctgacaata attaatagca
    15061 ttgcagttac agatttactg ggaatagtcc cctatgaact ttataactac caccgtgact
    15121 ttcttaattt aaaagagata aaactagaac atccgttacc ttcaataaag ctttacattt
    15181 cctataataa atcttcttta aataatttgg tgttctcccg atttattgac cgtctaaatg
    15241 agagtttttg atatataact acagacagct actataactt tcatctattt attcacagct
    15301 cgaatgaaaa ataaaacact ctgctttaca gtgtttactt tataattaac agtcttatct
    15361 cactgttcac cctgtaacgt tcagagagcg tcttcagatg tctatttata aaattcctct
    15421 tccactcaat attctggaag ctgcacgaga gcgtattacc tagacactca acaccctacc
    15481 ccgcgtttgt gtctcatttt ctggcggcaa agactctggt ttaatgctgc atctgacagc
    15541 cgaacttgcc cgacaaatgg gcaaaaaaat ctgcgttttg tttatcgact gggaggc
//
LOCUS       DADMGC010000094        15465 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.094, whole genome shotgun sequence.
ACCESSION   DADMGC010000094 DADMGC010000000
VERSION     DADMGC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.094"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(37..1230)
                     /locus_tag="JNZ73_002886"
     CDS             complement(37..1230)
                     /locus_tag="JNZ73_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY8733107.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEVDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     FEVTH"
     gene            complement(1429..1513)
                     /locus_tag="JNZ73_002887"
     tRNA            complement(1429..1513)
                     /locus_tag="JNZ73_002887"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1477..1479),aa:Leu,seq:caa)
     gene            1709..2728
                     /gene="ahr"
                     /locus_tag="JNZ73_002888"
     CDS             1709..2728
                     /gene="ahr"
                     /locus_tag="JNZ73_002888"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HAY8733108.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2857..4359
                     /locus_tag="JNZ73_002889"
     CDS             2857..4359
                     /locus_tag="JNZ73_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HAY8733109.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4478..5560)
                     /gene="lptG"
                     /locus_tag="JNZ73_002890"
     CDS             complement(4478..5560)
                     /gene="lptG"
                     /locus_tag="JNZ73_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY8733110.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5560..6660)
                     /gene="lptF"
                     /locus_tag="JNZ73_002891"
     CDS             complement(5560..6660)
                     /gene="lptF"
                     /locus_tag="JNZ73_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY8733111.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPIRRLRASFSRKGAV"
     gene            6927..8438
                     /gene="pepA"
                     /locus_tag="JNZ73_002892"
     CDS             6927..8438
                     /gene="pepA"
                     /locus_tag="JNZ73_002892"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAY8733112.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8572..9015
                     /gene="holC"
                     /locus_tag="JNZ73_002893"
     CDS             8572..9015
                     /gene="holC"
                     /locus_tag="JNZ73_002893"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HAY8733113.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9015..11870
                     /gene="valS"
                     /locus_tag="JNZ73_002894"
     CDS             9015..11870
                     /gene="valS"
                     /locus_tag="JNZ73_002894"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAY8733114.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11924..13120)
                     /locus_tag="JNZ73_002895"
     CDS             complement(11924..13120)
                     /locus_tag="JNZ73_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HAY8733115.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSAGMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNSVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILTFLDLLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13313..13816
                     /locus_tag="JNZ73_002896"
     CDS             13313..13816
                     /locus_tag="JNZ73_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8733116.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13862..14278)
                     /gene="rraB"
                     /locus_tag="JNZ73_002897"
     CDS             complement(13862..14278)
                     /gene="rraB"
                     /locus_tag="JNZ73_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HAY8733117.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14440..15453
                     /gene="argF"
                     /locus_tag="JNZ73_002898"
     CDS             14440..15453
                     /gene="argF"
                     /locus_tag="JNZ73_002898"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAY8733118.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3985 a         3943 c         3982 g         3555 t
ORIGIN      
        1 gaggtgagca tatcacctct gttcaggtgg ccaaattcag tgtgtcactt caaacatcaa
       61 cctacgctcc ccaagatgtt ccgccttatg gatgtaagct gctcgcacag agctacgctc
      121 ctggtgactc atctgccgct ctaccgcatc cctcgaccat aatcccgact caatcaacga
      181 actacaagcc atcgtcctga agccatgccc acaaacctcg accttcgtgt cataccccat
      241 tacgcgcaaa gccttgttca ccgtattctc actcatcggc ttacgtggat cgtgatcgcc
      301 cacaaaaatc agctctcgat ttccgctcat gcttttgatc ttttccaaaa tacttagggc
      361 ctgccgcgac aaaggtacaa ggtgtggtgt ccgcatcttc gagccacgct gagaatgctt
      421 gactccttcc agttgctcac gctcacctgg gattgtccac atagccgttt caaaatctac
      481 ttctgaccag cgggcaaaac gcagctcgct cgaacggatg aaaaccaata aagtgagttc
      541 aaccgccagt cgagttaacg gtctgccaga atagtgatct atgcgatgaa gtaattcagg
      601 tatacggttc agttcaagag ctgcacggtg ctgtcttttc gccgtagcaa ccgcacctgc
      661 aatctcttgc gcggggttgt agtcgattaa gccgctctga acggcaaagc gcataatcgc
      721 ggtagtacgc tgctgtaaac gggcggcaac ttcgagccgc ccggatgact cgactgcttt
      781 aatgggtaca agaagatccc gcgtcttcag ttccgcaatg ttccgcttac cgatagcagt
      841 aaagagatta tcctccagac ttttcaaaac acgagcgcta tgcgatgccg accacttctg
      901 attgctggcg tgccagtctc tggcgactac ttcaaacgtt atcgcctctt gttcctgttc
      961 taccttaaca gctttcttgt tttcactggg atcgacacca ttagctaaaa gcttacgagc
     1021 ctcatcccgg cgtgccctgg catccgccaa cgacacttcg gggtatttcc ccaatgcaag
     1081 catcttctct ttgccgccaa agcgataacg tagccgccag tatttggagc cgttagggtg
     1141 aaccagcaaa accatgcctt caccgtcggt cagcttatag gcttttgctt caggcttagc
     1201 cgaacgaacc ttcacatcac tcagagccat gatgagtatc ctttcaaggg ttctgtgtgg
     1261 gtacaaacat tatcgaaccg ggaaataccc gcagttgtac ccgcatcagt aagttgatgt
     1321 agattgaatc aggttgactt aggttgagtg aaaaagcgag gaaagccttg cggatactgg
     1381 attttaggca caaaaaaaga cgtccgttga cgtctattga tgttccgatg gtgccgaagg
     1441 ccggactcga accggcacgt atttctacgg ttgattttga atcaactgcg tctaccgatt
     1501 tcgccacttc ggcactgaag aggtatgcgg aaaacgttgt ggattatacc tgtcgcacgc
     1561 caccatgcaa gcgccagctt gcacccacct cgctaagtgc tgaaattttc agcattacac
     1621 tcagcgatat gtaacctttg tcacactcca ggcaccccgc cctgccatgc tctacacttc
     1681 ccaaacaaca ccagagaagg acccaaaaat gtcgatgata aaaagctatg ccgcaaaaga
     1741 agcgggcggc gaactggaag tttatgagta cgatcccggt gagctgaagc cacaagatgt
     1801 tgaagtgcag gtggattact gcgggatttg tcattccgat ctgtcgatga tcgataacga
     1861 atggggattt tcacaatatc cgctggttgc cgggcatgag gtgattgggc gcgtggtggc
     1921 actcgggagc gccgcgcagg ataaaggttt gcaggtcggt cagcgtgtcg ggattggctg
     1981 gacggcgcgt agctgtggtc actgcgacgc ctgtattagc ggtaatcaga tcaactgcga
     2041 gcaaggtgcg gtgccgacaa ttatgaatcg cggtggcttt gccgagaagt tgcgtgcgga
     2101 ctggcaatgg gtgattccac tgccagaaaa tattgatatc gagtccgccg ggccgctgtt
     2161 gtgcggcggt atcacggtct ttaaaccact gttgatgcac catatcactg ctaccagccg
     2221 cgttggggta attggtattg gcgggctggg gcatatcgct ataaaacttc tgcatgcaat
     2281 gggatgcgag gtgacggcct ttagttctaa tccggcgaaa gagcaggaag tactggcgat
     2341 gggtgccgat aaagtggtga atagccgcga tccgcaggca ctgaaagccc tgtcggggca
     2401 gtttgatctc attatcaata ctgtgaacgt cagcctcgac tggcagcctt attttgaggc
     2461 gctgacctat ggcggtaatt tccatacggt cggtgcggtt ctcacgccgc tgtctgttcc
     2521 ggcctttacg ttaattgcgg gcgaccgcag catctctggt tctgctaccg gcacgcctta
     2581 tgagctgcga aagctgatgc gctttgccgc ccgcagcaag gttgcgccga caaccgaact
     2641 gttcccgatg tcgaaaatta acgacgccat tcagcatgta cgcgatggta aagcgcgcta
     2701 ccgagtagtc ctgaaagccg acttctgatc cctttgccgg gtgctgtcgc ccggcactta
     2761 aatctctttg gaaggcgtct cgcaatctgc tgaatttagc ggtgaagagt gcacagccgt
     2821 tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc
     2881 cgcacgccgg acacagaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt
     2941 actggcgcaa cggggacggg taaaaccgtt acgctgcaaa aactggcaga gtcattgtcg
     3001 gaaatcggcg tgccggtgtt tatggctgat gtgaaaggcg atctgaccgg tatcgcgcag
     3061 gcaggaacgg cgtcggaaaa actgctcaca aggcttaaaa atatcggcgt caatgactgg
     3121 caaccgcatg ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccacacggtg
     3181 cgggcgacgg tttcggatct ggggccgctg ttgctggcgc gactgttgaa tctcaacgat
     3241 gtgcaatctg gcgtgctgaa tattatcttc cgcattgcgg acgatcaggg attgttgctg
     3301 ctcgacttta aagatttgcg ggcgattacc cagtacatcg gcgataacgc caaatccttc
     3361 cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg cggattactg
     3421 tcgctggaac agcaaggcgc agcacacttc tttggtgagc cgatgctgga tatcaaagac
     3481 tggatgcgca ccgatgccaa cggtaaaggc gttatcaata tcctcagcgc cgagaaactt
     3541 tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgctttcaga gttgtatgaa
     3601 caattgccgg aagcgggcga tctggagaaa ccaaaactgg tgtttttctt cgacgaggca
     3661 catctgctgt ttaacgatgc accgcaggta ctgctggata agattgagca ggtgataagg
     3721 cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc tgatattccg
     3781 gataatgtgc tcggccagct cggtaatcgt gttcagcacg ctttgcgagc ttttacgcca
     3841 aaagatcaga aagcagtgaa gacagcggcg caaaccatgc gggccaatcc ggcatttgat
     3901 accgaaaagg caatccagga actggggacc ggcgaggcgt taatctcgtt tctcgatgca
     3961 aaaggaagtc cttctgtggt ggaacgggcg atggtgatcg caccttgttc gcgaatgggg
     4021 ccggtgacgg aagatgagcg taatggcctg attaatcact ccccggtgta tggcaaatat
     4081 gaagatgagg tggaccgcga gtccgcctat gagatgctgc aaaaaggctt tcaggccagt
     4141 accgagcagc aaaataatcc ccccgtgaaa gggaaagagg tggcggtgga tgacggtatt
     4201 cttggtggat tgaaggatat tttgtttggc actaccggac cacgcggcgg gaagaaagat
     4261 ggtgtggtgc aaacaatggc gaaaagcgcc gctcgccagg tgacgaatca gattgtacgc
     4321 gggatgttgg ggagtttgct gggggggaga aaaagataag tcagacgtga tctgtgcccc
     4381 tcaccctaac cctctcccca aaggggcgag gggaccgttc gaactcgtat ttgactttgt
     4441 caacaataat aaagggagct ttcgctccct tgttagatta cgattttctc attaacagcc
     4501 acaggctgat taagaagaag ctggcacttg gcaacagtgc gccgatgatc ggcgggatgc
     4561 cgtaaaccaa cgtcagcggg ccgaagatct ggtccagtac gtagaagaca aaaccgaagc
     4621 tgatacctgt gaccacacgc acgcccatcg gtacgctacg cagtgggcca aagataaacg
     4681 acagcgccat cagcatcatc accgccacgg atagcggctg gaagattttg ctccacatgt
     4741 tgagctgata acgtccggca tcctgaccgc tcgacttcag atacttcacg tagttgtgca
     4801 aaccactgat ggagagtgca tccgggtcca gcgccaccac gcccagtttg tctggcgtga
     4861 ggtcggtttt ccaggtgccg ctcaccgtct gcgaaccggt aatctgtttc ggattggtca
     4921 gatcagattc atcaacctgc gacagacgcc agactttatg ttccgggtca aacttcgcgg
     4981 tagcggcata acgtacggat tgcagacgac gattctcgtt aaaggcataa atactgatgc
     5041 cacctaacac ctcgtcacct ttaacccgct caatgtagac gaagttgttg ccatctttcg
     5101 cccataaacc ttgctgggta gagagcaacg agccgccgta catcgcctgc gcacggtagt
     5161 tacgcgccat ctgttcgccc tgcggcgcga cccattcgcc aatcgccatc gtcagcaaga
     5221 ccagtggaat ggcggttttc atcaccgaca gcgctacctg catacgggta aaaccagaag
     5281 cctgcatcac caccagttcg ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa
     5341 gaagagccgc catcgggaag aagatctgca catctttcgg cacgctcagc aaggtataca
     5401 ttcctgcgcc taacgcgtcg taactcccct gcccggcttt tttcagctga tcgacaaatt
     5461 tgataatgcc cgacagcgac accagcatga acagcgtcat catgatggtg gtgaaaatag
     5521 ttttaccgat atagcggtca agtacgccaa aaggttgcat cacaccgctc ctttacgcga
     5581 aaaactggcg cgcaggcggc ggatcggcac ggtgtcccaa aggttgagaa caatcgccaa
     5641 agccagataa atcaggttaa cggtccacat ccacagtgtc gggtccagct tacctttacc
     5701 gccgttcgat ttcagggagg tctggatcag gaagaaaagt agatacagca gcatggctgg
     5761 cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg ctcaacggta caaccataag
     5821 tgccatcata aacacggtga ataccaacgt gatacgccag ttcagttctg cgcgagcacg
     5881 atcggtgtca gtgttccaca atgtgcgcat gtccatctgg tcggtatcgt tcgggtcgag
     5941 cgccaccgcc tggtgaccaa tgatcgcctg ataatcctgg aaatccgtaa tgcggaaatc
     6001 acgtaacagt gcagtgcctt cgaagcgtgt tccctggttg agagtgacga cctgggagcc
     6061 gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc acagaaggac gtgcattacc
     6121 ttttggtcga atttgtgcga ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat
     6181 gaacagcacc gagctgccat tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat
     6241 gccagggttc gctttcgctt ctgctaacac ttcatcctga tgacgcgatg accacggtcc
     6301 cgcccacatc acgttaaccg ccgcgacgat tgccgtgaat accgcaagga tcattgccgc
     6361 tttcaccaga accgctttgc tcaagccgca ggcatgcatt accgtaattt cactttcggt
     6421 atacagtttg cccagcgtca tcagcagccc gaggaacagg cttaatggca ggataagctg
     6481 cgccatttcc ggcacgccca acccgagaag ggagagcacc agattcgccg gaatatcgcc
     6541 gtcaaccgct gcgccgagga tcctcactaa cttttgacag aagaagatca aaagcaagat
     6601 gaagagtatc gccagctggc ttttgagcgt ctcccgcacc agatatctta tgattatcac
     6661 tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt agcttgtttc atggcttaaa
     6721 cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga taattatact caacggattc
     6781 acctctcaga ttttgttctg acgtgccaat gccgtaataa cgttaagatt aacacgaagt
     6841 catcgcaaca gcggacatga gttacgaaag cttgcaattc tatctgtagc caccgccgtt
     6901 gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt aaaaagcggt agcccggaga
     6961 aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg
     7021 cagaacagct cgataaaatc agcgatgggt acatcagcgc cctgctacgc cggggcgaac
     7081 tggaaggaaa accggggcag acattgttgc tgcaccatgt tccgaatgta ctttccgagc
     7141 gaattctcct tattggttgc ggcaaagaac gtgagctgga tgagcgtcag tacaagcagg
     7201 ttattcagaa aaccattaat acgctgaatg atactggctc gatggaagcg gtctgctttc
     7261 tgactgaact gcacgttaaa ggccgtaaca actactggaa agtgcgtcag gctgtcgaga
     7321 cagcaaaaga gacgctctac agtttcgatc agttgaaaac gaataagagc gaaccgcgtc
     7381 gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc
     7441 gcgcgatcca gcacggtctg gcgattgccg ccgggataaa agcagcgaaa gatctcggta
     7501 atatgccgcc gaatatctgt aacgccgctt atctcgcctc tcaggcgcgc cagctggctg
     7561 acagctacag caagaatgtc atcacccgcg ttatcggcga acagcagatg aaagagctgg
     7621 gaatgcactc ttatctggcg gtcggtcagg gttcacaaaa cgaatcgctg atgtcggtga
     7681 ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt
     7741 taaccttcga ctccggcggt atctccatca agccttcaga aggcatggat gagatgaagt
     7801 acgatatgtg cggcgcggcg gcggtttacg gcgtgatgcg tatggtcgcg gagctgcaac
     7861 tgccgattaa cgttattggc gtgctggcag gctgtgaaaa catgcctggc gggcgtgcct
     7921 atcgtccggg cgatgtgtta accaccatgt ccggtcaaac cgttgaagtg ctgaacaccg
     7981 atgccgaagg ccgcctggta ctgtgcgacg tgttaactta cgttgaacgt tttgagccgg
     8041 aagcagtgat tgacgtggcg acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcata
     8101 ttactggtct gatggcgaac cataatccgc tggcccatga actgattgcc gcgtctgaac
     8161 aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga gtatcaggaa cagctggagt
     8221 ccaattttgc cgatatggcg aacattggcg gtcgtcctgg tggggcgatt accgcaggtt
     8281 gcttcctgtc acgctttacc cgtaagtata actgggcgca cctggatatt gcaggaaccg
     8341 cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt
     8401 tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt ttgcgtttta ccctcaccct
     8461 aaccctctcc ctaagggaga gggaaccgat cgagcgcaat agaaattcat tgctcatctt
     8521 tcagggtgag gaatgcagca atatttaaat ccaccacaag aagccccata tatgaaaaac
     8581 gcaacgttct accttctgga caatgacacc accgtcgatg gcttaagcgc cgttgagcaa
     8641 ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta agcgcgtact catcgcctgt
     8701 gaagatgaaa agcaggctta ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc
     8761 tttgttccgc ataatttagc gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc
     8821 tggccgcaaa aacgcagcag cagcccgcgc gatatattga ttagtctgcg aacaagcttt
     8881 gcagattttg ccaccgcttt cacagaagtg gtagacttcg ttccttatga agattctctg
     8941 aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg
     9001 gcaacctgga aataatggaa aagacataca acccacaaga tatcgaacag ccgctttacg
     9061 agcactggga aaagcagggc tactttaagc ctaatggcga tgaaagccag gaaagtttct
     9121 gcatcatgat cccgccgccg aacgtcaccg gcagtttgca tatgggtcac gccttccagc
     9181 aaaccatcat ggataccatg atccgctatc agcgcatgca gggcaaaaac accctgtggc
     9241 aggtcggtac tgaccacgcc gggatcgcta cccagatggt cgttgagcgc aagattgccg
     9301 cagaagaagg taaaacccgt cacgactacg gccgcgaagc tttcatcgac aaaatctggg
     9361 aatggaaagc ggaatctggc ggcaccatta cccgtcagat gcgccgtctc ggcaactccg
     9421 tcgactggga gcgtgaacgc ttcaccatgg acgaaggcct gtccaatgcg gtgaaagaag
     9481 ttttcgttcg tctgtataaa gaagacctga tttaccgtgg caagcgcctg gtaaactggg
     9541 acccgaaact gcgtaccgct atttctgacc tggaagtgga aaaccgcgaa tcgaaaggtt
     9601 cgatgtggca catccgctat ccgctggctg acggcgcgaa aaccgcagac ggtaaagatt
     9661 atctggtggt tgcgaccact cgcccggaaa ccctgctggg cgataccggt gtagctgtta
     9721 acccggaaga tccgcgttac aaagatctga ttggcaaata tgtcattctg ccgctggtta
     9781 accgtcgtat tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc accggctgcg
     9841 tgaagatcac cccggcgcac gactttaacg actatgaagt cggtaaacgt cacgccctgc
     9901 cgatgatcaa catcctgacc tttgacggtg atatccgtga aagcgcccag gtgttcgata
     9961 ccaaaggtaa cgaatctgac gtttattcca gcgagatccc ggcagagttc cagaaactcg
    10021 agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga tgcactcggc ctgctggaag
    10081 aaattaaacc gcacgacctg accgttcctt acggcgaccg tggcggcgtg gttatcgaac
    10141 cgatgctgac cgaccagtgg tacgtgcgtg ccgatgtact ggcgaaaccg gcagttgaag
    10201 cggttgagaa cggcgacatt cagttcgtac cgaagcagta cgaaaacatg tacttctcct
    10261 ggatgcgcga tattcaggac tggtgtatct ctcgtcagct gtggtggggt caccgtatcc
    10321 cggcatggta tgacgaagcg ggtaatgttt atgttggccg caacgaagaa gaagtgcgca
    10381 aggaaaataa cctcggtgcc aacgttgccc tgcgtcagga cgaagacgtt ctcgacacct
    10441 ggttctcttc tgcactgtgg accttctcta cccttggctg gccggaaaat accgacgccc
    10501 tgcgtcagtt ccacccaacc agcgtgatgg tatctggttt cgacatcatc ttcttctgga
    10561 ttgcccgcat gatcatgatg accatgcact tcatcaaaga tgaaaatggc aaaccgcagg
    10621 tgccgttcca cactgtttac atgaccggtc tgattcgtga cgacgaaggt cagaagatgt
    10681 ccaaatccaa gggtaacgtt atcgacccgc tggatatggt tgacggtatt tcgctgccag
    10741 aactgctgga aaaacgcacc ggcaatatga tgcagccgca gctggcggac aaaatccgta
    10801 agcgcaccga gaaacagttc ccgaacggca ttgagccgca cggcaccgat gccctgcgct
    10861 tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa ctgggatatg aagcgtctgg
    10921 aaggctaccg taacttctgt aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca
    10981 cagaaggtca ggattgcggc ttcaacggcg gcgaaatgac gctatcgctg gcggaccgct
    11041 ggattctggc ggagttcaac cagactatca aagcgtaccg cgaagcgctg gacagcttcc
    11101 gcttcgatat cgccgcaggc attttgtatg agttcacctg gaaccagttc tgcgactggt
    11161 atctggagct gaccaagccg gtaatgaacg gtggaacaga agccgaactg cgcggtactc
    11221 gccatacgct ggtgactgta ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt
    11281 tcatcaccga aaccatctgg cagcgtgtga aagtactttg cggtatcact gccgacacca
    11341 tcatgctgca gccgttcccg cagtatgatg catctcaggt tgatgaagcc gcactggccg
    11401 acaccgagtg gctgaaacag gcgatcgttg ctgtacgtaa catccgtgca gaaatgaaca
    11461 tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg cagcgcggat gcagaacgtc
    11521 gcgtaaatga aaaccgtggc ttcctgcaaa cgctggcgcg tctggaaagc atcaccgtgc
    11581 tgcctgccga tgacaaaggt ccggtttccg ttaccaagat catcgacggc gcagagctgc
    11641 tgatcccgat ggctggcctc atcaacaaag aagatgagct ggcgcgcctg gcgaaagaag
    11701 tggcgaagat cgaaggtgaa atcagccgca tcgagaacaa gctggcaaat gaaggctttg
    11761 ttgcccgcgc accggaagcg gttatcgcga aagagcgtga gaagctggaa ggttatgcag
    11821 aagcgaaagc caagctgatt gaacagcagg ctgttatcgc cgcactgtaa tcaacacagt
    11881 gatgaaaacg aaggccggag catgctccgg ccttttttat ctcttacaga aatggtaaag
    11941 aaggcatgac tccccgagaa atactggcta agaagccttt atctggttgt tcttctttat
    12001 tgattaaagg aaggtcatcc aaatcgccca gcaaatacgt attttttgcc tgccagtcaa
    12061 tcatccatat tttcagcagt gggtaagcca gaccacccgt aatcccggat atcaccacca
    12121 acgcacacat gcgataaagc ataccgtggt acgttaaagt tgagcgaaaa cgaatacgcc
    12181 catcattcag tgacaggttg ctcataaaat gatttcgcaa cgacaccgtt aaataactgg
    12241 tgctaacagc aatcccaaaa taataaatta actgcgcgat tatcatttta cgttgcattt
    12301 ccataaattg ctgtaaattc tcaatatcat catttgcata cccagaacta tcatactcat
    12361 ttaatatttg atcgaagata atataaccag caacagcgag aaaaggtaat aaatctaaaa
    12421 atgtgaggat ggcatatttt atacaatacg cagtatctaa tttcaccttg aaacgatgta
    12481 taccgaaact ggtattgctc cagagaaaac tcattaccag gctatataaa gtaccattaa
    12541 aaataccaat ggaaacaata ccaactattg ccatcagaac cacagatata ataacactac
    12601 ttgaactatt ggcatgtagc atctttgtcg agataaagaa aacagtcccc atcccaatgg
    12661 ccattaaaat cggcaagaaa aaggttaccc accagaaccc tttcaaagag caattaaaac
    12721 taaatcttac gctgttgaga ctggtcatta aggcctgata acgcagtcct tttgctgcca
    12781 taaatataag caaaaccaac agtaataaag tcaaaacaca gccaacaagc ggcatacctg
    12841 ctgacactgt cattaagatt gcgaaataga aaaaaacaaa aacaagacaa ctaacaaaaa
    12901 cattcccacc ggtaattcca taagaaaatc gttgtccgtt aacttccata ttagcataaa
    12961 gataacgctt acatttcatt aatgcccatg gtaaatagat acctagcgtt ataatcgtta
    13021 acaacacatt caccacacaa ataagaaaat atctctcacc tgtaccatga aaaacaaaag
    13081 aatgggacgg aacatccatt tcattaataa cttgagccat aaaaatcccc tgaattaaaa
    13141 aatccattga tataatttat agatacagac agtaaaatgt tctgccacgt tacaatactc
    13201 ttatcagtaa taggctggca aaaactttat tgctttataa gacgcttgcg cacgtaagaa
    13261 gagagtggta ttaaacaaat gtataattac tttattttta ttgggttaaa ctatgaataa
    13321 cattgcgccg caatcacctg taatgcgtcg cctgacgctg caggataatc ctgctatcgc
    13381 cagcgtcatc cgtcaggttt acgcagagta tggtcttacc gccgataaag gctacaccgt
    13441 tgccgatccg aatctcgacg aactgtatca agtgtacagc cagcctggcc atgcttattg
    13501 ggtggttgaa tacaatggcg aagtggtcgg cggcggcggg attgcgccat taacaggtag
    13561 tgaagcggac atttgcgaac tgcagaaaat gtactttctt cccgctattc gcggtaaagg
    13621 gctagcgaaa aaactggcct taatggcgat ggagcaggct cgagagatgg gcttcaaacg
    13681 ctgctatctg gaaacgaccg cttttttaaa ggaagccatt gcgctttatg agcatttggg
    13741 ctttgagcat atcgactatg cgcttggctg cacgggccat gtcgattgtg aagtgcggat
    13801 gctacgcgaa ctctaaaaaa atgcccgcta gtatgtgcta tcgggcattt taatcaatta
    13861 attagtggcg aaccccgtca tcgtcttcat cgacaaaatc ttcatcgtcg ccatcttcgc
    13921 cgttaggatc ttcaaagtaa gtgccccaac cgtcgtactc aacgtcaaat ttctccgcca
    13981 gcgtcatcag ttgttcaacc tgggcatcga tcagatcggc attcaacgcg cactcgctga
    14041 ggatgtcgca gcaaatcacg atatcaccat cttcaacttc cagctcttct ggatcggtca
    14101 cttcgtaacc gagtttaaac gcttcaactg ccgctttttc cagagtttcc agatcgtctg
    14161 cggaaagatg gtgttcgatg gtgtacagcg cgtccggatc gctgccatct tccagtaatt
    14221 cttcaataat caaacgtgtt tcttcacgct gttcttccag ttgttccggg tttgccatgg
    14281 ctcattcctc ttaatgtcct gccgatactc ttattgtcac acaccactga cattgcctcc
    14341 acctttgtga caaagattta tgctttagac ttgcaaatga ataatcatac acataaattt
    14401 aattttaatt cattgaggcg ttagccacag gagggatcta tgtccgggtt ttatcataag
    14461 catttcctga aattactcga tttcacgcca gctgaactca acagcctgct gcagttagcc
    14521 gcgaagctga aagctgataa gaaaagcggt aaagaagaag ccaaactcac tggtaaaaac
    14581 atcgcgctca tcttcgaaaa agactcgact cgtacccgat gctctttcga agttgccgca
    14641 tatgaccagg gtgctcgcgt tacttatctt ggcccaagcg gtagtcagat tggtcataaa
    14701 gagtcgatta aagacactgc ccgcgtgctt ggtcgcatgt atgacggtat tcagtatcgc
    14761 ggctatggtc aggagattgt cgaaacactg gcggaatacg ctggcgtgcc ggtatggaac
    14821 ggcctgacca atgagtttca ccccacgcag ttgctggcgg atctcctcac catgcaggag
    14881 catttacccg gtaaagcatt caacgaaatg acgctggtct atgcaggtga cgcacgtaac
    14941 aacatgggca attcgatgct cgaagctgcg gcgcttaccg gtctggattt gcgtctggtc
    15001 gcgccacagg catgctggcc agaagctgcg ctggttacgg aatgccgcgc cctggcacaa
    15061 caaaatggcg ggaatattac gctgacggaa gatgtagcta aaggagttga aggtgctgac
    15121 tttatctata ccgatgtatg ggtgtcgatg ggtgaagcaa aagaaaaatg ggctgaacgc
    15181 attgcattac tgcgtgatta tcaggtgaac agcaagatga tgcagttgac cggtaacccg
    15241 gaggtcaaat tcctccactg cctgcccgcg tttcatgacg accaaacgac gcttggcaaa
    15301 aaaatggcgg aagagtttgg cctacatggc ggaatggaag tgactgatga ggtcttcgag
    15361 tctgccgcca gcattgtatt tgatcaggcg gaaaaccgca tgcataccat caaagcggtg
    15421 atggtcgcga cgctcagtaa attaaataat taatatctct ataaa
//
LOCUS       DADMGC010000095        15441 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.095, whole genome shotgun sequence.
ACCESSION   DADMGC010000095 DADMGC010000000
VERSION     DADMGC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.095"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="JNZ73_002899"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="JNZ73_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HAY8733119.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="JNZ73_002900"
                     /pseudo
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="JNZ73_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="JNZ73_002901"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="JNZ73_002901"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAY8733120.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPVAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="JNZ73_002902"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="JNZ73_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HAY8733121.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="JNZ73_002903"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="JNZ73_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HAY8733122.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="JNZ73_002904"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="JNZ73_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAY8733123.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="JNZ73_002905"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="JNZ73_002905"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAY8733124.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="JNZ73_002906"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="JNZ73_002906"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAY8733125.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="JNZ73_002907"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="JNZ73_002907"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAY8733126.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="JNZ73_002908"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="JNZ73_002908"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAY8733127.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="JNZ73_002909"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="JNZ73_002909"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAY8733128.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKLRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="JNZ73_002910"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="JNZ73_002910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAY8733129.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="JNZ73_002911"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="JNZ73_002911"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAY8733130.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="JNZ73_002912"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="JNZ73_002912"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAY8733131.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="JNZ73_002913"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="JNZ73_002913"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAY8733132.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..>15441
                     /gene="atpC"
                     /locus_tag="JNZ73_002914"
     CDS             15215..>15441
                     /gene="atpC"
                     /locus_tag="JNZ73_002914"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HAY8733133.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPG"
BASE COUNT         3794 a         3880 c         4041 g         3726 t
ORIGIN      
        1 attacccccc tttgagacta ttttagccac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttatgctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag ccagcctaca ggatgtcggt
     1561 gcacagattc gccaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct agacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt cagcttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagccg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgtagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgctcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcaactggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagaattgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 aggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggcggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggcg caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagttat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaagtcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacaacat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccaggta tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccaag caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gggtggtttt tgttgtgtaa atgtcattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgcaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcttggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaataa actccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggctg
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctgg cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggca ggcgttatca tccgagcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaaccttgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggcg gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca a
//
LOCUS       DADMGC010000096        15031 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.096, whole genome shotgun sequence.
ACCESSION   DADMGC010000096 DADMGC010000000
VERSION     DADMGC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.096"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..240
                     /locus_tag="JNZ73_002915"
     CDS             <1..240
                     /locus_tag="JNZ73_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HAY8733134.1"
                     /translation="TLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYM
                     PALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            250..888
                     /locus_tag="JNZ73_002916"
     CDS             250..888
                     /locus_tag="JNZ73_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HAY8733135.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLGRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            891..2054
                     /locus_tag="JNZ73_002917"
     CDS             891..2054
                     /locus_tag="JNZ73_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY8733136.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2138..3762
                     /locus_tag="JNZ73_002918"
                     /pseudo
     CDS             2138..3762
                     /locus_tag="JNZ73_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
     gene            complement(3879..4154)
                     /gene="yjfN"
                     /locus_tag="JNZ73_002919"
     CDS             complement(3879..4154)
                     /gene="yjfN"
                     /locus_tag="JNZ73_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HAY8733137.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4303..4632)
                     /gene="bsmA"
                     /locus_tag="JNZ73_002920"
     CDS             complement(4303..4632)
                     /gene="bsmA"
                     /locus_tag="JNZ73_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HAY8733138.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4814..5563
                     /gene="yjfP"
                     /locus_tag="JNZ73_002921"
     CDS             4814..5563
                     /gene="yjfP"
                     /locus_tag="JNZ73_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY8733139.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5560..6315)
                     /gene="ulaR"
                     /locus_tag="JNZ73_002922"
     CDS             complement(5560..6315)
                     /gene="ulaR"
                     /locus_tag="JNZ73_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HAY8733140.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6423..7487)
                     /gene="ulaG"
                     /locus_tag="JNZ73_002923"
     CDS             complement(6423..7487)
                     /gene="ulaG"
                     /locus_tag="JNZ73_002923"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAY8733141.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7842..9239
                     /gene="ulaA"
                     /locus_tag="JNZ73_002924"
     CDS             7842..9239
                     /gene="ulaA"
                     /locus_tag="JNZ73_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAY8733142.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9255..9560
                     /gene="ulaB"
                     /locus_tag="JNZ73_002925"
     CDS             9255..9560
                     /gene="ulaB"
                     /locus_tag="JNZ73_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAY8733143.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9570..10034
                     /gene="ulaC"
                     /locus_tag="JNZ73_002926"
                     /pseudo
     CDS             9570..10034
                     /gene="ulaC"
                     /locus_tag="JNZ73_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
     gene            10048..10698
                     /gene="ulaD"
                     /locus_tag="JNZ73_002927"
     CDS             10048..10698
                     /gene="ulaD"
                     /locus_tag="JNZ73_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAY8733144.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10708..11544
                     /locus_tag="JNZ73_002928"
     CDS             10708..11544
                     /locus_tag="JNZ73_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAY8733145.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11544..12230
                     /locus_tag="JNZ73_002929"
     CDS             11544..12230
                     /locus_tag="JNZ73_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HAY8733146.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12359..12634)
                     /locus_tag="JNZ73_002930"
     CDS             complement(12359..12634)
                     /locus_tag="JNZ73_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY8733147.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12961..13356
                     /gene="rpsF"
                     /locus_tag="JNZ73_002931"
     CDS             12961..13356
                     /gene="rpsF"
                     /locus_tag="JNZ73_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAY8733148.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNIEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13363..13677
                     /gene="priB"
                     /locus_tag="JNZ73_002932"
     CDS             13363..13677
                     /gene="priB"
                     /locus_tag="JNZ73_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HAY8733149.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13682..13909
                     /gene="rpsR"
                     /locus_tag="JNZ73_002933"
     CDS             13682..13909
                     /gene="rpsR"
                     /locus_tag="JNZ73_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAY8733150.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13951..14400
                     /gene="rplI"
                     /locus_tag="JNZ73_002934"
     CDS             13951..14400
                     /gene="rplI"
                     /locus_tag="JNZ73_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAY8733151.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14471..15028)
                     /locus_tag="JNZ73_002935"
     CDS             complement(14471..15028)
                     /locus_tag="JNZ73_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY8733152.1"
                     /translation="MNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDVE
                     SFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQLR
                     TVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRSD
                     RMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3511 a         3663 c         4050 g         3807 t
ORIGIN      
        1 acattgttag gttacgttat cccattatcc agtgcggcaa tcaatgcggt gagtattcca
       61 gactatttcg cctggggcgg gattgcactg gtgattcagt tactcgtttt tgctggtgtc
      121 agactttata tgcccgcatt aagcgaaaaa attattaatc acaataccgc agcaggaatg
      181 tttatgggaa ccgccgcgct ggctggcggt atttttaacg cagcttgtat gacatggtaa
      241 tggacgatca tggccagaaa acgcaaatca agaaataaca gtaaaattgg tcacggagcg
      301 atcagtcgca ttggtagacc gaataatcct tttgaaccgc gtcgcaatcg ctacgcacaa
      361 aaatacttaa cgttggcgct aatgggcggt gccgcttttt tcgtattgaa aggttgtagc
      421 gatagtagcg atgtcgataa cgacggcgac ggaacgtttt acgcgacagt gcaggattgt
      481 attgatgacg gtaataatgc cgacatttgc gcgcgtggct ggaacaacgc caaagcggca
      541 ttttatgccg atgttccaaa gaatatgact cagcagaact gtcagtctaa gtacgaaaat
      601 tgctactacg acaatgttga acagagttgg atcccggtcg tttctggatt tttgttaggc
      661 cgggttattc gtaaagatcg cgatgagccg ttcgtctata acagcggcgg atcctcattt
      721 gcttcccgcc ccgtctggcg caacacttcc ggtgattact cctggcgctt cggttctggc
      781 aaaaaagagt cttactcttc aggcggcttt accaccagaa aagcgtcgac cgtttcgcgc
      841 ggcggctatg gtcgttcttc cagcgcccgt gggcattggg gaggctaatc atgctgagac
      901 acaacgttcc tgtgcggcgg gatctggacc agatcgccgc taacaacggt ttcgactttc
      961 atatcatcga caatgaaatc tattgggacg agagtcgggc ttaccgtttt actctgcgcc
     1021 agattgaaga gcagatcgaa aaaccgactg cggaactgca tcagatgtgc cttgaggtgg
     1081 tggatcgcgc ggttaaagac gaagagatcc tgacgcaact ggcgatcccg ccgttgtact
     1141 gggatgtgat cgccgaaagc tggcgcgcac gcgatccttc gctgtatggc cggatggatt
     1201 ttgcctggtg tggcaatgcg ccggtgaagc tgctggagta caacgccgat acgccgacct
     1261 cgttgtatga gtcggcttat ttccagtggt tgtggctgga ggatgcccgg cgcagcggca
     1321 ttattccgcg tgatgccgat cagtacaacg ccattcagga acgcctgatt tcgcgcttta
     1381 gtgagcttta cagtcgggaa ccgttttatt tttgctgctg tcaggacacc gatgaagaca
     1441 ggagtaccgt gctgtacttg caggactgcg cccagcaggc agggcaggag tcgcggttta
     1501 tctacattga agatctcggt ttgggcgtcg gcggcgtact gaccgatctt gatgataatg
     1561 tcatccagcg tgcatttaag ctgtatccgc tggagtggat gatgcgtgac gataacggtc
     1621 cgctgctgcg caagcgtcgc gagcaatggg tggagccgtt atggaaaagt atcttgagta
     1681 ataaagggct aatgccgctg ctttggcgct tcttccctgg tcatcctaac cttcttgcat
     1741 cctggtttga gggtgaaaaa tcgcagattg ccgctggcga aagctatgtg cgtaaaccga
     1801 tctactcgcg cgaaggcgga aacgtcacca ttttcgacgg tcagaataac gtcgttgacc
     1861 acgctgatgg tgattacgcc gatgaaccga tgatctacca ggcgtttcaa cctctgccgc
     1921 gatttggcga tagctacaca ctcatcggta gctggattgt cgatgatgaa gcgtgcggaa
     1981 tggggatccg tgaagataac acactgatca ccaaagacac ctcacgtttc gttccgcatt
     2041 acattgctgg ataagaatgt tttagcaatc tctttctgtc atgaatccat ggcagtgacc
     2101 atactaatgg tgactgccat tgatggaggg agacacagtg cactggcaaa ctcacaccat
     2161 ttttaatcaa cctataccat taaataacag caacttatac ctgtctgatg gcgcgctttg
     2221 cgaagcggta acgcgtgaag gtgctggctg ggatagcaat tttctagcca gtattggtca
     2281 gcagttagga acggctgaat cccttgaact ggggcggctg gcgaatgtaa acccgcctga
     2341 actgttgcgc tacgatgccc aggggcggcg tctggacgat gttcgtttcc atcccgcatg
     2401 gcacctgctg atgcaggcgt tatgtaccaa tcgggtgcac aatcttgcct gggaagaaga
     2461 cgctcgctcc ggcgcatttg tggcgcgcgc ggcgcgtttt atgttacatg gcaggttgag
     2521 gctgggtcgt tatgtccaat aacgatgacc tttgccgcca cgccattgtt gttacagatg
     2581 ttacccacgc cgtttcagga ctggaccacg ccgctgctga gcgatcgcta cgattctcac
     2641 ttattgccag gtgggcaaaa acgcggtttg ttgattggca tgggaatgac ggaaaagcag
     2701 ggcggttccg atgtcatgag caacaccatc cgtgcagagc gtttggaaga tggctcttat
     2761 cggctggtgg ggcataaatg gtttttctcg gtgccgcaaa gcgatgcgca tctggtgctg
     2821 gcgcagaccg cgggcggtct gtcctgcttt tttgtgccgc gctttttgcc tgacgggcaa
     2881 cgcaacgcga ttcgcctcga gcggctgaaa gataagctgg gtaatcgctc taacgccagt
     2941 tgcgaagtgg agtttcagga tgccattggc tggttattgg ggcaggaagg ggaaggaatt
     3001 cgtctgatcc tgaaaatggg ggggatgacg cgttttgatt gcgccctggg tagccatgcc
     3061 atgatgcgcc gtgcattttc gctggcgatt tatcatgcac atcaacgcca tgtttttggt
     3121 aatccattga tccaacagcc ccttatgcgt catgtcttaa gtcgcatggc acttcagctt
     3181 gaagggcaaa cggcgttgct gtttcgtctt gcgcgagcgt gggaccggcg tgccgatgcc
     3241 aaagaagccc tgtgggcgcg tttatttacg cctgcggcaa aatttgtgat ctgcaaacga
     3301 ggtatgccgt ttgtggccga agcgatggag gtgctgggcg gcattggtta ttgcgaggag
     3361 agcgagctgc cgcggcttta ccgggagatg ccggtaaaca gtatttggga aggttccggc
     3421 aatattatgt gcctggatgt gttgcgcgtt ctcaataagc aagcgggcgt atacgactta
     3481 ttgtcggaag catttgtgga agtgaaaggg caggatcgct attttgatcg cgcggttcgt
     3541 cgtttacagc agcagctgcg taagccagct gaagaactgg ggcgagagat tactcatcag
     3601 ctattcctgc tgggctgcgg tgcgcaaatg ttgaaatatg cttctccgcc aatggcgcag
     3661 gcgtggtgtc aggtgatgtt agatacgcgc ggcggcgtac ggttgtcaga gcagatccag
     3721 aatgatttat tgctgcgggc gacgggggga gtgtgtgtgt aagcgtatac gactgatgcg
     3781 acgctggttt cgattaacta aatgaaatat gtgaaaattg taggccggac aaggcgctcg
     3841 cgccgcatcc ggcattgttc atttgctgta gatgacactc atgcatacag tatcgcctgt
     3901 acgcgccagt tatcaggctg ctggtcttcg tacatggtaa taatgcggta ccatgacgca
     3961 ccttgttcat cggcctttaa cgccacgata cgttccacgt cctgcggatc ccctgaaata
     4021 ttgctaactg agatctgacc tatttcattc aggcccgtca cgcagtcagc actggcgaat
     4081 tctgcagact gtgccgtggc gctcgcagga taagtcacta attgcagcga gggtgaggca
     4141 agtaattgtt tcatcgtcag ctccattttt cgtttcccca catgttgcag ggcattccat
     4201 cgcggtaata aacgcccaag cttccagccg gtaatttatt gtgcacaggt aaacgcatag
     4261 ggacgcaaag caatgtaaag ctgactatgt aagttgtctg atttatttac ggtacaaaat
     4321 ggcttgtgaa taccactgtc ctgtcacgat ggtttcgtcc accatgacga caacgtaata
     4381 atcagctttt gcagcgacag cttttgctct gatttctgct aatgcgtcat ccggagaacc
     4441 ccgaaccatc gtgcttacgc tacctattcg ctgtaaccct tgcgtctggt cgcggcgaat
     4501 ctcttgcgga tggtccgtta ctggcggtgc tggctgtggc gtaccttgca gtgcgctaca
     4561 ggcacttaac atcaacacca ataataaact ggcaaaccgg taaataacgc tattacgttt
     4621 cctgctaacc atagtgtagt gccttattaa tttaactttg ggggaatgtt cccgaattgt
     4681 tacctggtac gcgattttcg aatgaaaacg tgacgaaagc gtaacccaca tctcaacatt
     4741 atgaactaat accgccagca gaaacttgtg ctaaggattt cttcatgtca taccagacaa
     4801 aaaggagaga cagatgattg aaatagaatc acgcgagctg gcagatattc ccgttcttca
     4861 tgcttatcct gtcgggcaaa aagatacccc gttaccgtgc gtaatttttt atcacggctt
     4921 tacttcatcc agcctggtgt atagctattt tgccgttgcg ctggcgcagg ctggtttgcg
     4981 ggtgatcatg ccggatgcgc ccgatcacgg tagccgtttt agtggcgacg aagcgcggcg
     5041 gttaaatcaa ttctggcaaa tcttgctaca aagtatgcag gaattcacta ctttacgtgc
     5101 ggcaatagcc gaagaaaact ggctgcttga tgaccgtctg gcagtcggtg gcgcgtcgat
     5161 gggcgcgatg acggcactgg gaattaccgc tcgccacccc acggtgaaat gtaccgccag
     5221 catgatggga tcgggctatt ttacatcgct cgcccgttca ctgtttccac cgctgatacc
     5281 tgaaacggca gcacagcaga atgaattcaa taaaattgtc gcgccgctgg cagagtggga
     5341 agcgacaaac cacctagaac aacttgggga cagacctcta ctgctgtggc atggcctcga
     5401 cgatgacgtt gtgcctgccg acgaatcact acgtttgcag caggccttaa gcgagacggg
     5461 acgggataaa ctgctaacct gttcatggca gccaggcgtg cgtcaccgca ttacgcctga
     5521 ggcgttagat gctgccgtga catttttccg ccagcatctt taaacacgca gaatgctgac
     5581 cccttgcgct tccagttgtt gcaggatttc cgggttagca tttttgccgg tgatcagcat
     5641 atcgatttga tcggcacggc taaaaagcat tcccgcgcgt tcgccaatct tactgctatc
     5701 aaccagtacc accagtttcc ctaccacgct cagcatcttc tgctctgcca ttgctgtcag
     5761 catatcggtt ttatacaacc cttctgcggt cagccctttt ccgctggtaa acatccagtg
     5821 cccggcatag agactgtttt cgctgccctg cgggcttaaa gtgatggact gacttttgtt
     5881 gtactgtcca cccataatga tcacgctgtc atgttcttga tcgatcaggt aatttgccag
     5941 cggtagataa ttagtgatga tttgcactgg cttgccacac atttcccgcc caagcagaaa
     6001 cgcggtggag ccgcagttga tgactacgct ttcgcccgga ttaaccagct gcgaggccgc
     6061 tttagcaata cgtacttttt catcgtgatt ctgcgcctga tgcagattca tcggcgtcca
     6121 gcgcgggcgc tgttgggtaa tagcttctgc gccattgcgc acctttttca gtttgccgct
     6181 ttcgccaagt ttattgatat cgcgtcgcgc agtggcaggc gaaattccca gacgctcaac
     6241 gactttctca acggtcacaa agcccaattg tgcgagcatt tccaggagga tttgatgtct
     6301 ttgtgcttca gtcatgagct attccgataa aatttgattt ttttagatga tatttgaaat
     6361 agccaggaaa tactacgccg gatagcgcga acttctccac gctatccggt ataaacgaca
     6421 gattacagga atgacttgaa cggcagatcc ggttcaatag tgaagcaatc atcgaaaccg
     6481 cgcggatagt ggtactcgaa gttgtcttta tccagcggcc aggtaaattt gccgccaacc
     6541 tgccagataa acggcttgaa gccatacttc agacgatctt ttttcatctc ccacagcacg
     6601 cggatctctt gcggatcggc ctggaagttt gaccagatgt cgtggtggaa cgggataacc
     6661 actttcgcat tcagggcttc acccatacgc agcatatcgg cgctggtcat tttgtcggta
     6721 ataccgcgcg ggttctcacc atacgatcct aacgccacat cgatctgatg ttcgttaccg
     6781 tgcttcgcat aatagttaga gtagtgggaa tcgccactgt gatataggga accgccaggg
     6841 gttttgaaca ggtagtttac tgcgcgatcg tccatgccat ctggcagtac gccagccgct
     6901 ttttgatcgg caggcagggt gatcagtgca gtacggtcga aagcgtcaag cgcatgaatt
     6961 tcaatgtctt tcacttttac aacatcgccc ggtttgacca cgatgcaacg ctctttcggt
     7021 acgccccagc caatccacaa atcaacgcag gttttcggtc caatgaatgg tacgtcatca
     7081 gcacagttct gcatcacggc agcagcgacg ttaacgtcga tatgatcgtt gtgatcgtga
     7141 gtcgccagta ccgcgtcgat ctggcgaatc gcaaacggat caagaacaaa cggggtggtg
     7201 cgtaggtttg gctgcagttt tttcacgcca gccatgcgct gcatctggtg accctgtttc
     7261 attaacgggt taccgtgact ttgtttgcca gtgccgcacc agaaatcaac gcaaacgttg
     7321 gtgccacctt ctgatttcaa ccagatcccg gtgcagccta gccaccacat cgcaaatgtg
     7381 ccaggggcga cctgctcttg ttcaatttct tcattcagcc agctacccca ctccgggaaa
     7441 gtgctcagga tccaggattc tcgggtgata cttttcactt tactcatcgc catttacctt
     7501 catgatagtt caattcgaat caatatgtga ttggttttga ttaatcctga cactattttt
     7561 tcaggaaggc aatgaccatt ttttgacttt ttgccaggga agttgttgtt gattttgagt
     7621 gtagaaagat ttaatggaat gtatagttaa attaactaaa tgaatttaaa taaataattt
     7681 tttcgttgtg tgaatcccac tctatccgtg tggaattatt tgcgggtcgc gtcacattta
     7741 atcataaata atcttgttgt gattactttt gaaaattaga gtgagtgcac aacattccgg
     7801 gtgtgcggaa tacccggtta cctcttcttc aggagatcgt tatggagatc ctctacaaca
     7861 tctttaccgt gttttttaac caggtcatga ccaatgcccc gttgttgctg ggtattgtga
     7921 cctgtctggg ctacatccta ctgcgcaaaa gtgtcagcgt tattattaaa ggcacgatta
     7981 aaaccataat tggtttcatg ttgttgcagg cagggtccgg catcctcacc agcaccttca
     8041 aaccggtggt ggcgaaaatg tccgaagtct acggcattaa cggcgcaatt tccgatactt
     8101 acgcgtcgat gatggcaacc atcgaccgca tgggcgatgc ctatagctgg gtaggttacg
     8161 cggtgttgtt agcgctggcg ctgaacatct gttacgtgct gctgcgtcgc attaccggta
     8221 ttcgtaccat catgctgact ggtcatatca tgttccagca ggcggggctg attgccgtta
     8281 cgttgtttat tttcggctac tccatgtgga caaccattat ctgcacggcg attctggttt
     8341 cgctctactg gggcattacc tccaacatga tgtacaagcc gactcaggaa gtgacggacg
     8401 gctgcggttt ctccatcggt caccagcaac agtttgcatc attaattgcc tataaagttg
     8461 cgccgttcct cggcaaaaaa gaggaaagcg ttgaagacct caaactgcca ggctggctga
     8521 atattttcca cgacaacatc gtctccacgg cgattgtcat gaccatcttc tttggtgcca
     8581 ttctgctctc cttcggtatc gacaccgtgc aggcgatggc aggcaaagtg cactggacgg
     8641 tctatatcct gcaaaccggt ttttccttcg cggtggcgat cttcatcatc actcagggtg
     8701 tgcgcatgtt tgtggcggaa ctctctgaag cgtttaacgg tatctcccag cgcctgatcc
     8761 ctggtgcggt tctggcgatt gactgtgcgg ctatttatag cttcgctccg aacgccgtgg
     8821 tctggggctt tatgtggggc accatcggtc agctgattgc ggttggcatc ctggtcgcct
     8881 gcggttcctc gatcctgatt atcccaggct ttatcccgat gttcttctcc aacgccacca
     8941 tcggcgtgtt cgctaaccac ttcggcggct ggcgtgcggc gctgaagatt tgtctggtga
     9001 tggggatgat cgaaatcttt ggttgcgtct gggtggtgaa acttaccggt atgagtgcct
     9061 ggatggggat ggcggactgg tcgattctgg caccgccgat gatgcagggc ttcttctcca
     9121 tcggtatcgc ctttatggcc gtcatcattg taattgcact ggcttatatg ttcttcgctg
     9181 gccgcgcact gcgcgcagaa gaagatgcag aaaaacaatt ggcagaacag tctgcttaat
     9241 aaggagtttt gattatgacc gtacgtattc tggctgtgtg tggcaacgga caaggcagtt
     9301 ccatgatcat gaagatgaaa gtggaccagt ttttaaccca atcaaacatt gaccacacgg
     9361 taaatagctg cgcggttggc gaatacaaaa gcgagttgag cggcgcggat atcatcatcg
     9421 cttctacgca cattgcgggc gaaatcaccg tgaccggcaa caaatacgtg gttggcgtgc
     9481 gcaacatgct ctctcctgcc gactttggcc cgaaactgct ggaagtgatc aaagcgcatt
     9541 tcccgcagga tgtgaagtaa ggacgcgcca tgaaattaca tgattcgctg gcggagaata
     9601 aatccatccg cctgcaggct gaagctgaga catggcagga cgcagtaaaa atcggcgttg
     9661 acctgctggt ggcggcggat gttgtcgagc cgcgttacta ccaggcgatt ctggatgcgg
     9721 ttgaacagca cggtccgtac tttgtgctcg caccaggcct ggcaatgccg cacggtcgcc
     9781 cggaagaggg cgttaagaaa accggttttg cgctggtgac gctgaaaaag ccgctggagt
     9841 tcaaccacga agataacgac ccggtggata tcctcatcac catggcggtg gtcgatgcca
     9901 acacgcacta ggaagtgggc atcatgcaga tcgtcaacct gtttgaagat gaagaaaatt
     9961 tcgaccgttt acgtgcctgc cgtaccgagc aggaagtact ggatctcatt gaccgcacca
    10021 acgcggcagc ttaagaagga attgaagatg tcattaccga tgttgcaagt cgcgctggac
    10081 aaccagacta tggatagcgc ctacgaaacc actcgcctga ttgccgaaga agtcgacatt
    10141 atcgaagtgg gtaccattct gtgcgtgggc gaaggcgtgc gtgcggttcg cgatctgaaa
    10201 gcgctctacc cgcacaaaat cgtgctggca gacgccaaaa ttgccgatgc gggcaaaatt
    10261 ctttctcgta tgtgcttcga agccaacgct gactgggtga ccgtgatttg ttgtgcggat
    10321 atcaacaccg ccaaaggtgc gctggacgta gcaaaagagt ttaacggcga cgtgcagatc
    10381 gaactgaccg gttactggac ctgggaacag gcgcaacagt ggcgcgacgc aggtattcag
    10441 caggtggttt atcaccgcag ccgtgacgcg caggccgctg gcgtggcgtg gggtgaagcc
    10501 gatatcactg cgatcaaacg tctttccgat atgggcttca aagtcaccgt caccggtggc
    10561 ctggcgctgg aagatctgcc gctgttcaaa ggcattccaa ttcatgtgtt tatcgccggt
    10621 cgcagcatcc gtgatgccgc ttctccggtt gaagccgcgc gtcagttcaa acgttccatc
    10681 gccgaactgt ggggctaagg agcggatatg ttgtccaaac aaatcccgct tggcatctat
    10741 gaaaaagcgc tccccgccgg ggagtgctgg ctggaacgcc tgcgactggc aaaaacgttg
    10801 ggcttcgatt ttgtcgagat gtcggtagat gaaactgacg agcgcctgtc gcgcctcgac
    10861 tggagccgcg agcagcgtct ggcgctggtg aatgcgattg ttgaaaccgg cgtgcgtgtg
    10921 ccgtccatgt gcctttctgc ccatcgtcgt ttcccgttgg gcagcgaaga tgacgcggtg
    10981 cgggcgcagg ggctggagat tatgcgtaaa gccatccagt tcgcccagga cgttggcatt
    11041 cgcgtgatcc agctggcggg ctatgacgtt tactatcagg aagccaataa cgaaacgcgt
    11101 cgtcgtttcc gtgacggcct gaaagagagc gttgagatgg cgagccgcgc acaggtgacg
    11161 ctggcgatgg agatcatgga ttatccgttg atgaactcca tcagcaaggc gctgggttac
    11221 gcgcactatc tcaacaaccc gtggttccag ctctacccgg atatcggcaa cctgtcggcg
    11281 tgggacaacg atgtgcagat ggagttgcag gccggaatcg ggcatatcgt cgcggtgcat
    11341 gtgaaagaca ccaaaccggg cgtcttcaaa aacgtgccat ttggcgaagg tgtggtggat
    11401 ttcgaacgtt gcttcgaaac gctcaaacag agtggctatt gcgggccgta cctgattgag
    11461 atgtggagcg aaacggcgga agacccggcg gcagaagtgg tgaaagcgcg catggccaaa
    11521 gcaggcatgg tggaggcggc ataatgcaaa agctaaaaca gcaggtgttt gaagccaaca
    11581 tggatctgcc gcgctatggg ctggtgacct ttacttgggg caacgtcagc gctatcgacc
    11641 gcgaacgcgg gctggtggtg atcaagccca gcggcgttgc ctacgaaacc atgaaagcag
    11701 acgatatggt ggtggtcgat atgagcggca aggtggtgga aggggagtat cgcccatctt
    11761 ccgacactgc gacgcatctc gaactctacc gtcgttaccc gtcgcttggc ggcattgtcc
    11821 atacccactc cactcatgcc accgcatggg cgcaggcggg gctggcgatc ccggcattag
    11881 gcaccacgca cgctgactac ttctttggtg atatcccgtg tacgcgtgga ttaagcgaag
    11941 aagaggtgca gggtgagtat gaactgaaca ctggcaaagt gattatcgaa acgctgggca
    12001 acgccgagcc gctgcatacg ccgggaattg tggtgtatca gcacggaccg ttcgcctggg
    12061 ggaaagatgc ccacgacgcg gtgcataacg cggtagtgat ggaagaagtg gcgaaaatgg
    12121 cgtggattgc gcgcagtatt aatccacaac tcaatcatat cgacagctac ctgatgaata
    12181 aacacttcat gcgtaagcac ggtcctaacg cttattacgg gcagaagtaa aacacgcgct
    12241 gcggaaattt ccttcctcgg gagataactg gtctaattcc gcagccgttt ttcaaaaaaa
    12301 gccccctgcg aagggggcaa agcaaactat ggcaatgttt cgttggttat acctggtgct
    12361 agcgataaat atccgcgctg gcgtgcatat tgccgttact ccccggttcc cgcatcagaa
    12421 ttacgtggta gtacgttgcg ccttgcgcat ctgtttcttc atttaatgcc tgacgtgctt
    12481 cgctttcagt ggcgaaatta tgattgatat aaatcacgcc taagctttgc acatcgtcca
    12541 tatttctggc ctggtggtta ttaatttcaa tggctgccca tgtatttgca cttagcaaaa
    12601 gcacagccag aagggctaaa actcgactga acatagatac ctcctcgacg gctgactttg
    12661 tgtgctctcc tctgtgatga tcttctgatt taattttaat caatgataaa gaagttgatg
    12721 gtgaccattt ctgatgcagt tgttcaaaaa aacactgtga cggaatgtga tgaacttcaa
    12781 atcagcggtt tagaggttat ttgcgaaagg ggagatttat ttctgctccg cccttgagtt
    12841 tagcggggta tgtcagtact ataacgcgtc atttttcagc cgacctttaa cacgttcctt
    12901 gcctccccgg gattcggctg acccagacag gaggctgaat aatccgtaag gagcaattcg
    12961 atgcgtcatt acgaaatcgt ttttatggtc catcctgacc agagcgaaca ggttccgggc
    13021 atgatcgagc gctacactgc tgccatcact ggtgcagaag gcaagatcca ccgtctggaa
    13081 gactggggcc gccgtcagct ggcttacccg atcaacaaac tgcacaaagc acactacgtt
    13141 ctgatgaaca ttgaagctcc gcaggaagtg atcgatgagc tggaaactac cttccgcttc
    13201 aacgatgccg ttatccgcag catggttatg cgtaccaagc acgctgttac cgaagcatct
    13261 ccgatggtta aagcgaaaga cgagcgccgt gagcgtcgcg atgatttcgc aaacgaaacc
    13321 gctgatgatg ctgaagctgg ggattctgaa gagtaatttc tgatgaccaa ccgtctggtg
    13381 ttgtccggca ccgtgtgcag gactcccctt cgaaaggtca gtccatcagg aattcctcac
    13441 tgccagttcg tgcttgagca tcgttctgtg caggaggaag ccggctttca ccggcaggca
    13501 tggtgtcaaa tgcccgttat tgttagcgga cacgaaaacc aggccattac tcacagtata
    13561 acggtcggca gtcgcataac cgttcagggg ttcatttcat gccacaaggc aaagaacgga
    13621 ctgagcaaaa tggttttgca cgccgagcag attgaattga tagattctgg agactagcca
    13681 tatggcacgt tatttccgtc gtcgcaagtt ctgccgtttc accgcggaag gcgttcaaga
    13741 gatcgactat aaagatatcg ctacgctgaa aaactacatc accgaaagcg gtaagattgt
    13801 cccaagccgt atcaccggta cccgtgcaaa ataccagcgt cagctggctc gcgctatcaa
    13861 acgcgctcgc tacctgtccc tgctgccgta cactgatcgc catcagtaat cggtcacggt
    13921 ccattaatac gactttgaga ggataaggta atgcaagtta ttctgcttga taaagtagca
    13981 aacctgggta gcctgggtga tcaggtaaac gttaaagcgg gctatgctcg taacttcctg
    14041 gtaccgcagg gtaaagctgt tccagctacc aagaaaaaca ttgaattctt cgaagcacgt
    14101 cgcgctgaac tggaagctaa actggctgaa gttctggcag ctgctaatgc tcgcgctgag
    14161 aaaatcaatg cactggaaac tgttaccatc gcgtctaaag ctggcgacga aggtaaactg
    14221 ttcggttcca tcggtactcg cgacatcgct gacgctgtaa ctgcagctgg cgttgaagtg
    14281 gctaagagcg aagttcgtct gccgaacggc gttctgcgta ccactggcga acacgaagtg
    14341 agcttccagg ttcacagcga agtattcgcg aaagtgatcg taaacgtagt agctgaataa
    14401 ttcgttattc aacgagacgt aaaaacgccg accattggtc ggcgttttgc tttctatttt
    14461 tcgtcaggca ttaatttcgc aagtagatcc attcaccatt ctcttgtgtc cataagtcag
    14521 gatggtttgc tggcgttaga tgcatacgat cactccttat gtctcggtcc tgcggcactc
    14581 gctcgaaacc taaacccgag tagattgcgg ctgaatttga taaggaacgt agaccaattt
    14641 tcaaatcatg gggtaaatca gaatacgcgt ttagcaatgc agcaatgatg aatccgaggg
    14701 cattagaaac gcctgtaaca gtgcgaagtt ggtcgatata aagatcgatt gttggtagtt
    14761 cagatgacca gcaattaggc atttgggcag gaagggttac aacgtatcct atcgtttggc
    14821 cggtatagtc tggagtacta taaccaggtc ccaaaacaac ctcgtgtatt gtgtatttac
    14881 tgttgggttc gaaggattcg acatcttgca ataaatattt cagttctgcg gttaacctcg
    14941 cacccgcgta agctttattg tatttctggg cgtgaatatt atctaatgta gcttcatggt
    15001 tccatccaaa agaattatca ccattcattg t
//
LOCUS       DADMGC010000097        15015 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.097, whole genome shotgun sequence.
ACCESSION   DADMGC010000097 DADMGC010000000
VERSION     DADMGC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.097"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..604)
                     /locus_tag="JNZ73_002936"
     CDS             complement(<1..604)
                     /locus_tag="JNZ73_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8733153.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNAAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQAESEVSTPVHPSASMVGLDAGVAKLASLSDGTVFEPV"
     gene            612..1112
                     /gene="tnpA"
                     /locus_tag="JNZ73_002937"
     CDS             612..1112
                     /gene="tnpA"
                     /locus_tag="JNZ73_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAY8733154.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(1151..1498)
                     /gene="grxD"
                     /locus_tag="JNZ73_002938"
     CDS             complement(1151..1498)
                     /gene="grxD"
                     /locus_tag="JNZ73_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HAY8733155.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1833..2660
                     /locus_tag="JNZ73_002939"
     CDS             1833..2660
                     /locus_tag="JNZ73_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAY8733156.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATAHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2788..3369
                     /gene="sodB"
                     /locus_tag="JNZ73_002940"
     CDS             2788..3369
                     /gene="sodB"
                     /locus_tag="JNZ73_002940"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HAY8733157.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(3515..4684)
                     /locus_tag="JNZ73_002941"
     CDS             complement(3515..4684)
                     /locus_tag="JNZ73_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8733158.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(4850..4939)
                     /gene="ynhF"
                     /locus_tag="JNZ73_002942"
     CDS             complement(4850..4939)
                     /gene="ynhF"
                     /locus_tag="JNZ73_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HAY8733159.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            5238..6263
                     /gene="purR"
                     /locus_tag="JNZ73_002943"
     CDS             5238..6263
                     /gene="purR"
                     /locus_tag="JNZ73_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HAY8733160.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(6260..7192)
                     /locus_tag="JNZ73_002944"
     CDS             complement(6260..7192)
                     /locus_tag="JNZ73_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8733161.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            7305..8516
                     /locus_tag="JNZ73_002945"
     CDS             7305..8516
                     /locus_tag="JNZ73_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HAY8733162.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRTALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8807..9955
                     /gene="cfa"
                     /locus_tag="JNZ73_002946"
     CDS             8807..9955
                     /gene="cfa"
                     /locus_tag="JNZ73_002946"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HAY8733163.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9995..10636)
                     /gene="ribE"
                     /locus_tag="JNZ73_002947"
     CDS             complement(9995..10636)
                     /gene="ribE"
                     /locus_tag="JNZ73_002947"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAY8733164.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10851..12224
                     /gene="mdtK"
                     /locus_tag="JNZ73_002948"
     CDS             10851..12224
                     /gene="mdtK"
                     /locus_tag="JNZ73_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HAY8733165.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(12265..13521)
                     /locus_tag="JNZ73_002949"
     CDS             complement(12265..13521)
                     /locus_tag="JNZ73_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733166.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYAYGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGDRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13829..13905
                     /locus_tag="JNZ73_002950"
     tRNA            13829..13905
                     /locus_tag="JNZ73_002950"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13863..13865,aa:Val,seq:gac)
     gene            13910..13986
                     /locus_tag="JNZ73_002951"
     tRNA            13910..13986
                     /locus_tag="JNZ73_002951"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13944..13946,aa:Val,seq:gac)
     gene            14094..14399
                     /locus_tag="JNZ73_002952"
     CDS             14094..14399
                     /locus_tag="JNZ73_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAY8733167.1"
                     /translation="MATLLQFHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            14525..>15015
                     /locus_tag="JNZ73_002953"
     CDS             14525..>15015
                     /locus_tag="JNZ73_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAY8733168.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATD"
BASE COUNT         3685 a         3869 c         3563 g         3898 t
ORIGIN      
        1 ttacaggctc aaagactgtg ccatctgaca gcgaggcaag tttagccacg ccagcatcca
       61 gcccgaccat tgatgctgaa gggtgaaccg gagttgatac ttcactttct gcctgaatac
      121 tgatgtacca cttaccgcag gactggctga cagtgacatt tttcacaact cccgtgacct
      181 gacggctgtt ccggtagcgc atccagccaa gtttcggcag aaaaatacgg ctgttttcct
      241 gatcgagctt aacgccctgc gggtagcgga atgcagcatt ctgtccccgc tttttgaatc
      301 ggggaaaagc agcccgcttc cggaagaagt ttttgtaagc ccgctcaagg tctttcagtg
      361 actgttgcaa tggctgtgag ggcgaatctt taagccattg cgtttcagtg gcgtttttcc
      421 actcaaccag ccaggaagcc attttcccgt aagggatgta tttatttccg gcctcatgat
      481 tctcattctg aagcgccaga gcacgattga aaacgaaacg acatgcgcct gcgaagcgcc
      541 tcatttcacg ctcctgttga ccaccgggtc ttaactggaa tttaaatgcc tgtaatcgtt
      601 tcacaggtca aatgatactt tggtctgtga aaaaagaaac cgatatccgg cgtggaaggc
      661 attgtgtttt cctgatgcat gttcacctgg tctttgtcac cagataccga cgccagattt
      721 ttgaccatga cacgacagaa aaactacgca cttacttttc aaatgtatgt gctgattttg
      781 cagctgaact ggttgaaatg gatggcgaac cagatcacgt ccatttgtta attaactatc
      841 ctcccaaact ggcgatatcc agtctggtaa acagcctcaa aggcatatcg aataggttac
      901 tgcgacgaga tcgaccagat attgcagtca ggtattacta caaaggcgtt ttgtggagtc
      961 ctggctattt tgccagtagc tgcgaaggtg cgccaatatc cgtcatccgc caatacattg
     1021 aacaacagca aacacctggt caggtggaaa accgcgcctt atatccccgc cctgaaggac
     1081 gaggttttac ggcgcaccgg ataaaggact gacggatgcc cgttcgcatc cgtcagtatt
     1141 gcaggacgga ttattccgcg tccggctctt cagacttgta tttagcggca gtttctttga
     1201 tcagctgctg cagttcgcca cgctgataca tttcgatcac gatatcacaa ccgccgacca
     1261 gctcgccgtc aacccacagt tgcgggaagg tcggccagtt agcatatttc ggcagttccg
     1321 cacgaatgtc cggattctgc agaatatcaa cataggcaaa acgttcgcca catgcggcaa
     1381 gcgcctggac tgcctgggca gagaaaccgc agctcggcag tttcggtgaa cctttcatgt
     1441 acagcaggat cgggttttca gcaatctggc gttggatttt ttcgatagtg gtgctcattg
     1501 tcttgcttcc tcaaacttct ttacggcagt aatctgacat tgtagcgggt cagtgcggca
     1561 tcggaaaata acattttcat cacgcttttg ctattttatc cctttgctct atttgttgca
     1621 tttcaaatat tcgtttcttc tctgatgcgc ggcaaactgg cgtatttata accattttta
     1681 gctttcactg ctgcattttt ttgcgctcgc caacgaaacg tattttttaa caataaaagc
     1741 tattaacttt ctcttcttct atgcattaga atcatcaagt tttgtaaatc agacgcaggc
     1801 atgatagacc tgccttaaca gagggacgct cagtggcgcg gataaaccgt atttcgatca
     1861 cgctctgtgc tttacttttt accaccctgc ctttaacgcc tatggcccat gcttcaaagc
     1921 aagccaggga gagttctgct accgctcata tcaccaaaaa agcagataaa aagaaaagca
     1981 cggcaaccac caaaaagacc cagaaagcca gccagaaaac agcgaaaaaa gccgccagta
     2041 aaagtacgac caaaagcaaa accgcttctt ccgttaaaaa atcttccatt accgcttcta
     2101 aaaacgccaa aactcgcagc aaacacgccg tcaataaaac ggcctcagcc agctttaccg
     2161 aaaagtgtac caaacgtaag ggctataaat cgcattgtgt gaaagtcaaa aatgccgcgt
     2221 caggaaccct tgccgacgcg cacaaagcga aggtgcaaaa agccacgaaa gtggcaatga
     2281 ataaactgat gcagcaaatt ggtaagccat atcgttgggg cggcagctca ccgcgtaccg
     2341 gttttgattg cagcggcctg gtttattacg cttataaaga tttggtgaaa attcgtattc
     2401 cgcgtacggc gaatgaaatg tatcacctgc gtgatgcagg acctatcgaa cgtagtgagc
     2461 tgaaaaacgg cgacctggtc tttttccgta ctcagggacg cggtacagcc gatcatgtcg
     2521 gcgtgtatgt cggcaacggc aaatttattc agtcaccgcg cacaggtcag gaaattcaaa
     2581 tcacttctct cagtgaagac tactggcagc gccactatgt tggcgctcgt cgggtaatga
     2641 ccccaaaaac acttcgctaa aactttaccc tgttgttacg gcaacagggt aagttcatct
     2701 tttgtctcac cttttaattt gctaccctat ccatacgcac aataaggcta ttgtacgtat
     2761 gcaaattaat aataaaggag agtagcaatg tcattcgaat tacctgcact accatatgct
     2821 aaagatgctc tggcaccgca catttctgcg gaaactatcg agtatcacta cggcaagcat
     2881 catcagactt atgtcactaa cctgaacaac ctgattaaag gtaccgcgtt tgaaggtaaa
     2941 tcactggaag agattattcg cagctctgaa ggtggcgtat tcaacaacgc agctcaggtc
     3001 tggaaccata ctttctactg gaactgcctg gcaccgaacg ccggtggcga accgactgga
     3061 aaagtcgctg aagctatcgc cgcatctttt ggcagctttg ccgatttcaa agcgcagttt
     3121 actgatgcag cgatcaaaaa ctttggttct ggctggacct ggctggtgaa aaacagcgat
     3181 ggcaaactgg ctatcgtttc aacctctaac gcgggtactc cgctgaccac cgatgcgact
     3241 ccgctgctga ccgttgatgt ctgggaacac gcttattaca tcgactatcg caatgcacgt
     3301 cctggctatc tggagcactt ctgggcgctg gtgaactggg aattcgtagc gaaaaatctc
     3361 gctgcataat aactgatggc aaatgcagca ttgcctgatg cgcttcgctt accatgccaa
     3421 cgcggatgag gcgttaacag cgcattcggc aagataaatc gcacgttgtc agcaactgta
     3481 acgcagaagg ttatccttct gcgtttttgt ttaattagct gttagcaacg caaactgttt
     3541 caggttgttt tctggctgac ataaacacca gcaataatgc cagtcccgcg acaatcgccc
     3601 ccgtcaccgg cacaaagctg tatcccagcc cagcggaaat taccgcacca ccagcagctg
     3661 ctcccagcgc atttccaaga ttaaaggcac caatattgac tgatgaagac agacctggcg
     3721 cttcactggc gacatgcatc acgcgcatct gtaacggagg tacaaccgca aaggttgctg
     3781 cgccccacac caccatgcta atagctgcgc cgaactcatt gcgggccagg aacgggattg
     3841 ccagcataat caccatcagc agcaacaaaa agcctttcaa cgtgccgtta actgaacgat
     3901 ctgccagttt gccgccgaga tagttaccga tagagaatcc gacaccaatc agcaccagca
     3961 ttgccgtgac gaacaccggt gttgcgtggg taatactttg cagtaccgga gagatatagg
     4021 tgtagagggt aaacattgca ccagctccca gtaccgtcgt cagcaatgca gacagcacct
     4081 gcggacgcat taataccgcc agctcttttt tcacttcagg tcgtgcccct gtaccacctt
     4141 taggtaatga gaagaacaga cttaccattg aaatcactcc cagccccgcc gttgccagaa
     4201 atgacatccg ccagccgatg gtttcaccca accaggtcgc cgccggcacg ccaccgatat
     4261 ttgccagggt taaccccata aacatagtgg caactgcgct ggcctgttta tgttttggca
     4321 ccacgcttgc ggccacgact gaacccaaac caaaaaatgc tccgtgattc aggctggtca
     4381 aaatacgtga aagcatcagg gtcatataat ccggcgcgat ggcggaaaga acgttgccta
     4441 gcgtgaaaat tgccatcagg aaaatcaacg cactgcggcg ggcacgatga gaaagtagaa
     4501 gcgtcatcag cggcgcgccc accattacgc caactgcata ggcactgatt aacattccgg
     4561 cagcgggaat cgagacatcc acaccgcgcg caatgacggg caacaagccc attggcgaga
     4621 actccgttgt cccgatacca aacgcgccaa tcgccagcgc cagcaacgga tagttaattt
     4681 tcatgcctta tctccacctc ttcgcgtcat tacgcgatat tcattaaagt ggcgaaagca
     4741 tgacagcaat cacaaaaaaa tgaaaataac aaaaagagaa aacacttttg ccattttgct
     4801 aacaaacagg aaggagatgc gagggagaac gcgctccctc gagaggaaat cagtgcagcg
     4861 cggcagtcaa acccacggct acgatcaaac cgaggacgat aatcgttgtt accagtgaaa
     4921 atttaaggtc ggtgctcatc aagttttctc cttttttatt accacacaaa aagtgatatt
     4981 acgcattttt acacactgtt atgaaaaaat ctcccgtcat ttataatgat aagtgttttt
     5041 accacttccc cttttcgtca agatcggcca aaattccacg cttacactat ttgcgtactg
     5101 gccattgacc ccttcctgac gctccgtgtc gtttttccgg cgtaccgcaa cacttttgtt
     5161 gtgcgtaagg tgtgtaaagg caaacgttta ccttgcgatt ttgcaggagc tgaagttagg
     5221 gtctggagtg aaatggaatg gcaacaataa aagatgtagc gaaacgagca aacgtttcca
     5281 ctacaactgt gtcacacgtg atcaacaaaa cacgtttcgt cgctgaagaa acgcgcaacg
     5341 ccgtgtgggc agcgattaaa gaattacact actcccctag cgcggtggcg cgtagcctga
     5401 aggttaacca caccaagtct atcggtttgc tggcgaccag cagcgaagcg gcctattttg
     5461 ccgagatcat tgaagcagtt gaaaaaaatt gcttccagaa aggttacacc ctgattctgg
     5521 gtaatgcgtg gaacaatctt gagaaacagc gggcttatct gtcgatgatg gcgcaaaaac
     5581 gcgtcgatgg tctgctggtg atgtgttctg agtacccaga gccgttgctg gcgatgctgg
     5641 aagagtatcg ccatatccca atggtggtga tggactgggg tgaagcaaaa gctgacttca
     5701 ccgatgcggt cattgataac gcgttcgaag gcggctacat ggccgggcgt tatctgattg
     5761 aacgcggtca ccgcgaaatc ggcgtcatcc ccggcccgct ggaacgtaac accggcgcag
     5821 gccgccttgc cggttttatg aaggcgatgg aagaagcgat gatcaaggtg ccggaaagct
     5881 ggattgtgca gggtgacttt gaacctgaat ccggttatcg cgccatgcag caaatcctgt
     5941 cgcagccgca tcgccctact gccgtcttct gtggtggcga tatcatggca atgggcgcac
     6001 tttgtgctgc tgatgaaatg ggcctgcgcg tcccgcagga tgtttcgctg atcggttatg
     6061 ataacgtgcg caacgcgcgc tattttacgc cggcgctgac cacgatccat cagccaaaag
     6121 attcgctggg tgaaacagcg ttcaacatgc tgttggatcg tatcgtcaac aaacgtgaag
     6181 aaccgcagtc cattgaagtg catccgcgtt tgattgaacg ccgctccgtg gctgacggcc
     6241 cgttccgcga ctatcgtcgt taatcacccg ttgcgggagt ctcttccggc tcccgcagcc
     6301 actccttatt cagcgtctca ctatcgccga gatactcaag cagccaggtt aacgcaggcg
     6361 acatatcatt ttgctgccat gtcagacaac atgccgaatc cgggaagggg ttttccagtt
     6421 caagtgctac ccacttcccc tcattaagcc acggtttggc gaaatgtgtt ggcaccatcc
     6481 ctatgcataa tcctgccgag atacaggttg ccgatgattc ccagtcaggt acgacaagtc
     6541 ttttttggtt atccagcaac caggtaatac gtttaggtag cgttcgcgag gtgtcttcgc
     6601 gcaccaacga cggccagttg cgcaacgtat catcgctgaa cgggccatcc atcaacgcca
     6661 gcgggtggtg actggcaaca acgcaactcc agcttagcat ccccatatcc cggaaggcat
     6721 aacgaccgcc taccggaatc gcgcgtgttg cgccaattgc cagttccacg cgcccgtcgg
     6781 aaagcgcatc ccagacaccg ttgaacactt cctgaaagac aagaagttca acatcatcaa
     6841 aatggcgata aaaatcaacg atcatctgcc gtgtacgttc tggcctgaca atattatcca
     6901 ctgcgatagc taactgaccg cgccagccgt tcgctatctg ctgacattgc tggcgggtga
     6961 tctgcatttt tttgacaaca gagcgccctt ctttgagaaa ccacgctcca gcagcggtca
     7021 gctccacatc acggtgccgt cgttcaaaga gcggtaccgc cagccactct tccagctgac
     7081 gcacggtata gctgaccgca gaaggaacgc gatgcagctc ctgtgccgca gcgctaaaac
     7141 taccattacg cgctaccgca tcaacaactt cgagtgaata ttctgaccac atagtcagcc
     7201 tgcaaaattt ttgaaaccag tcatcaaata ttaccgtttc acaacactaa tttcactccc
     7261 tacactttgc ggcggtgttt aattgagaga tttagagaat atacatgcaa cctgggaaaa
     7321 gatttttagt ctggctggcg ggtttgagcg tactcggttt tctggcaacc gatatgtatc
     7381 tgcctgcttt cgccgccata caggccgacc tgcaaacgcc tgcgtctgct gtcagtgcca
     7441 gccttagtct gttccttgcc ggttttgccg cagcccagct tctgtggggg ccactctccg
     7501 accgttatgg tcgtaaaccg gtgttattaa tcggcctgac aatttttgcg ttaggtagtc
     7561 tggggatgct gtgggtagaa aacgccgcca cgctgctggt attgcgtttt gtacaggctg
     7621 tgggggtctg cgccgcggcg gttatctggc aagcattagt gacagattat tatccttcac
     7681 agaaagtcaa ccgtattttt gcgaccatca tgccgctggt gggtctgtct ccggcactgg
     7741 ctcccctgtt aggaagctgg ctgctggtcc atttttcctg gcaggcgatt ttcgccaccc
     7801 tgtttgccat taccgtggtg ctgattctgc ctattttctg gctcaaaccc acgacgaagg
     7861 cccgtaacaa tagtcaggat ggtctgacct ttaccgacct gctacgttct aaaacctatc
     7921 gcggcaacgt gctgatatac gcagcctgtt cagccagttt ttttgcatgg ctgaccggtt
     7981 caccgttcat ccttagtgaa atgggctaca gcccggcagt tattggttta agttatgtcc
     8041 cgcaaactat cgcgtttctg attggtggtt atggctgtcg caccgcactg cagaaatggc
     8101 aaggcaagca gttattaccg tggttgctgg tgctgtttgc tgtcagcgtc attgcgacct
     8161 gggctgcggg cttcattagc catgtgtcgc tggtcgaaat cctgatccca ttctgtgtga
     8221 tggcgattgc caatggcgcg atctacccta ttgttgtcgc ccaggcgctg cgtcccttcc
     8281 cacatgcaac cggtcgcgcc gcagcgttgc agaacactct gcaactgggt ctgtgcttcc
     8341 tcgcaagtct ggtagtttcc tggcttatta gtatcagcac gccattgctc accaccacca
     8401 gcgtgatgtt atcaacagta gtgctggtcg cgctgggtta catgatgcaa cgttgtgaag
     8461 aagttgactg cccgaatcat ggcaatgccg aagtcgctca tagcgaatca cactgatcta
     8521 tatcgatata cttatactta ggctgctaac aaaattttgt tgtatgattg aaattagcgg
     8581 cctatactaa tttcgagttg ttaaagctac gataaatatt atgtttttac ggggacagga
     8641 tcgttcccga ctcactatgg atggtcattt cggcaagggt tcctcctttc cctctgttct
     8701 acgtcggatt atagactcgc ggttttttct gcgagatttc tcacaaagcc caaaaagcgt
     8761 ctacgctgtt ttaaggttct gatcaccgac cagtgatgga gaaactatga gttcatcgtg
     8821 tatagaagaa gtcagtgtac cggatgacaa ctggtaccgt atcgccaacg aattacttag
     8881 ccgtgccggt atagccatta acggttctgc cccggcggat attcgtgtga aaaaccccga
     8941 tttttttaaa cgcgttctgc aggaaggctc tttggggtta ggcgaaagtt atatggatgg
     9001 ctggtgggaa tgtgaccgac tggatatgtt ttttagcaaa gtcttacgcg caggtctcga
     9061 gaaccaactc ccccatcatt tcaaagacac gctgcgtatt gccggcgctc gtctcttcaa
     9121 tctgcagagt aaaaaacgtg cctggatagt cggcaaggag cattacgatt tgggtaatga
     9181 cttgttcagc cgcatgcttg atcccttcat gcaatattcc tgcgcttact ggaaagatgc
     9241 cgataatctg gaatctgccc agcaggcgaa gctcaaaatg atttgtgaaa aattgcagtt
     9301 aaaaccaggg atgcgcgtac tggatattgg ctgcggctgg ggcggactgg cgcactacat
     9361 ggcatctaac tatgacgtaa gcgtggtcgg cgtcaccatt tctgccgaac agcaaaaaat
     9421 ggctcaggaa cgctgtaaag gcctggatgt caccattttg ctgcaagatt atcgtgacct
     9481 gaacgaccag tttgatcgta ttgtttctgt ggggatgttc gagcacgtcg gaccgaaaaa
     9541 ttacgatacc tattttgcgg tggtggatcg taatttgaaa ccggaaggta tattcctgct
     9601 ccatactatc ggttcgaaaa aaaccgatct gaatgttgat ccctggatta ataaatatat
     9661 ttttccgaac ggttgcctgc cctctgtacg ccagattgct caatccagcg aaccccactt
     9721 tgtgatggaa gactggcata acttcggtgc tgattacgat actacgttga tggcgtggta
     9781 tgaacgattc ctcgccgcat ggccagaaat tgcggataac tacagtgaac gctttaaacg
     9841 gatgtttacc tattatctga atgcctgcgc aggtgctttc cgcgcccgtg atattcagct
     9901 ctggcaggtg gtgttctcac gcggtgttga aaacggcctt cgagtcgctc gctaaaggct
     9961 attctatcgc cccctcttcg ggggcgagtt cagatcaggc ttctgtacct ggttgattca
    10021 tggcattttc tcgtgccgcc agcacacgtt ctaccgtatc taccactgcc tgagtttgtg
    10081 gatcgatttc aatgttgacg cgtgcgccaa gttttttctt cccaagagtc gtgcgttcca
    10141 gtgtttccgg aattaaatgg acgcaaaaac gcgttggcgt gacttcgccg acggtcaggc
    10201 taataccgtc gatgccaata aatcctttgt acaggatata tttcatcaac tgactatcct
    10261 ggactttaaa ccagatctgg cgattatttt ccgaggttaa tattttcgcc acttcagcgg
    10321 tggtcataat atgacctgac attaagtgtc cgccaatttc atcactgaat ttcgccgcac
    10381 gctcaacgtt tacccaatcc cccactttta aatcgccaag attggtaatg cgtaacgttt
    10441 ctttcatcag gtcaaaactg acatggttgc cgttaatttc cgtcacggtc aggcagcaac
    10501 cgttatgcgc cacggaagca ccggtttcca ggccgtccag catgtggtcg ggtaactcca
    10561 ccacatgcgt acgaaaattt ggtttctcgt caatcgacac cagttttgcg gtgccctgta
    10621 caatccccgt aaacatactt acaactcctg aaatcagtta agacattctg ttcagcacaa
    10681 tagcaggtgg aaaacgccct taccagtgaa ggggtaagaa tggctatttt ttcactggag
    10741 aattaataaa tcctcgctac aatagactga atttcccctg cttcttcttt ttgctgccca
    10801 ttcaggcggc tttttagtct ctcatataac tacaaataaa aggtgttcac gtgcagaagt
    10861 atatcagtga agcgcgtctg ttattagcat tagcaatccc ggtgattctc gcgcaaatcg
    10921 cccaaactgc gatgggtttt gtcgataccg tgatggcggg cggctatagt gccaccgaca
    10981 tggcggcggt cgctatcggt acttctatct ggcttccggc gatcctcttt ggtcacggac
    11041 tgctgctggc attaacgccg gttatcgcgc aattaaatgg ttccggtcga cgtgagcgca
    11101 ttgcgcatca ggtgcgacaa ggtttctggc tggcaggttt tgtttccgtt ctcattatgc
    11161 tggtgctgtg gaatgcaggt tacattatcc gctccatgga aaacatcgat ccggctctgg
    11221 cggacaaagc cgtgggttat ctgcgtgcgt tgttgtgggg cgcgccggga tatctgttct
    11281 tccaggttgc ccgtaaccaa tgtgaaggtc tggcaaaaac caagccgggt atggtaatgg
    11341 gctttatcgg cctgctggtg aacatcccgg tgaactatat ctttatttat ggtcatttcg
    11401 gtatgcctga gctcggtggc gttggttgtg gcgtggctac tgcggcggtg tattgggtca
    11461 tgttccttgc catggtttct tacattaaac gcgcccgctc catgcgcgat attcgtaacg
    11521 aaaaaggcac cgctaagccc gatcctgcgg ttatgaaacg actgattcaa ctcggtttgc
    11581 cgattgcgct ggcactgttc tttgaagtga cactgtttgc cgtcgtggct ctgttagtgt
    11641 ctccgctcgg tattgttgat gtcgcaggac accagattgc cctgaacttt agttcactga
    11701 tgtttgtgct tccaatgtcg ctggcggcag cggtaactat ccgcgtaggt tatcgtctgg
    11761 gtcagggctc aacgctggat gcgcaaaccg ctgcgcggac cgggcttatg gtgggtgtct
    11821 gtatggcaac cctgacggcc attttcacgg tttcactgcg ggagcaaatc gccctgttgt
    11881 acaacgacaa tcccgaggtt gtaacgctgg ctgcgcattt gatgttgctg gcggcggtat
    11941 atcagatttc tgactcaatc caggtgattg gcagtgggat tttgcgtggt tataaagata
    12001 cgcgttccat tttctatatc acctttacag cttactgggt gctgggcttg ccaagcggct
    12061 atattctggc actgaccgat ctggtcgttg aacctatggg gccagcaggc ttctggatag
    12121 gctttattat cggcctgaca tcggcagcca ttatgatgat gctgcgtatg cggttcctgc
    12181 aacgtatgcc gtcagccatc attctgcaac gagcgtcccg ctaataaaga caaggcgcaa
    12241 ccttcacggg ttgcgcctgt gtttttacgc aggctggagc gttgcgccaa tcccgtcttc
    12301 gtctggctgt aatttcagag cgttacacag agtttgccga ctggcatctg ttatctcaac
    12361 gcgtatatcc ccgttaactt cccctttcac attgatgaag tcaggtgaaa tttctttcgc
    12421 actggtttgc aggcaaatcg ctccctctcc gcgcaggcgt ttcactttca gttgcacatg
    12481 cgttgccgca ccagtgatca gccacagttt tccgccattc agattgatgt taccaacaat
    12541 gaaagtgacg ctgtcacctt ttaccgtact gccgtcgaga atcaacacac cgccctgctg
    12601 tacagtaatc gttgattgtt gcagctctgc gccataacca ataatcattg aagcgccgtc
    12661 cagtaatgca acactcccgg tcagttcaac cggtgcgcga tcttcctcta cgacaaaatt
    12721 ttcgtccgcg ctggtgggtg acaacaggat cactgttcca ccttgtatta ccgcattttc
    12781 cagcgtaccg gcatgcactt cagcccgatc ccccacattc accgacaggt tttgcgtctt
    12841 accggaactg tagagctgaa ggccatccgt ccccagacga taaatggatc cattctcaac
    12901 aatagtgtca gagtcttcac caccgagatt attcatggtg ccaccagcaa gcaacgtatc
    12961 actggctttc ccggccagca taaaaacgcc gccctgatta atctcacaat tgctggcttc
    13021 tccacttgga tacaccaaca attcaccacc ttcatctacc acgaccgctg aggttgtccc
    13081 attaaccaac aggccgccct cttgatcgag aatgattttt tccgcgcgat gtccttccag
    13141 tacacgcagg ttaccgccat tttccagcaa caaaccgtag gcataaccct gatcaacgct
    13201 gaattcacct tcgggatggc ggccattaac cgtagcgagc gtagagagac taattgcgcc
    13261 tccctcggac tgaacaatat ctgtggcaat ggcaccgtcg ctgaccgata gcgtgccatt
    13321 gttaccgtac tgtgtgcatt tggcgatccc accggttgcc actttcaacc agccagcccc
    13381 cgtgaccacg gtattttcgg ctcttccgcc agcttcaaca atcaactcgc cttcagtaag
    13441 ttgcgtcgcg ccaatgacct cgccggtctt aacaatttgc acattcccgt cgctcatcaa
    13501 gtttttcgca tcagatccca tgatttattc ctttgctgca tctgtgtgcc tttattgcta
    13561 cctaagtgta aaggctacgg aggatttatc cacgacagat ttgagatggt ggcaaacaac
    13621 tctgtttaaa ctctgataca cgaattattg ggttgtatca gatgtaaatg cgatcctgaa
    13681 taaaaatcgc ccttgcaaat caacaaaata tagccaattt gcttaaaagc tctgcaaacg
    13741 gtcgaaatag cgcagaaaat tacgttttgc ctcttgccac ctttccactc tgccgctaat
    13801 attcgtcccc gttgtcacct acaacgttgc gttcatagct cagttggtta gagcaccacc
    13861 ttgacatggt gggggtcgtt ggttcgagtc caattgaacg caccatcctg cgtccgtagc
    13921 tcagttggtt agagcaccac cttgacatgg tgggggtcgg tggttcgagt ccactcggac
    13981 gcaccagatt ttcttaatct ggtcttttcc tttttccctc tgtttcttct ctgtatccaa
    14041 tacgttaaaa gatttacact gtcttcgtat gcgttatcag aaggagaatc gctatggcaa
    14101 ctttgttaca atttcatttt gcttttaatg gcccatttgg cgacgcaatg gctgagcagc
    14161 ttaaaccact tgctgagtcg attaatcagg aacctggttt tctgtggaag gtatggacag
    14221 aaagtgaaaa gaaccacgaa gccggtggga tctacctttt cactgatgaa aaaagcgccc
    14281 ttgcctatct ggaaaaacat actgcccgac tgaaaaatct cggcgttgag gaagttgtcg
    14341 ccaaagtttt cgatgtcaat gagccactta gtcaaatcaa tcaggcaaaa ctcgcctgac
    14401 agaatttaat caagggcggt tagcgccctt ttcatccctg tctgaaattt ctcaaattct
    14461 aaaaatctca accaaactta tctgataaca ctaaattcga aagaatgcgt acaggtaagt
    14521 aacaatgaaa aaaattgcta ttgtgggtgc cgggcctacg gggatctaca ccttattctc
    14581 gcttctacag caacaaactc cactttctat ttctatcttc gagcaggctg acgaggccgg
    14641 tgtcgggatg ccatacagtg atgaggaaaa ctcaaaaatg atgctggcaa atattgccag
    14701 tattgaaata ccgccgatta attgtacgta tctcgaatgg ctacaaaagc aagaagccag
    14761 ccatctccag cgttatggcg ttaaaaaaga aaccttgcac gatcgtcagt ttttaccgcg
    14821 aattctgctg ggcgaatatt tccgcgatca atttttacga ttagtagacc aggcacgaca
    14881 gcaaaaattt gcagtggctg tttatgaatc atgccaggtt accgatctgc aaattacaaa
    14941 tgctggcgtc atgctcgcta caaatcagga tttacccagc gagacgtttg atttagcggt
    15001 gattgccacg gatca
//
LOCUS       DADMGC010000098        14955 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.098, whole genome shotgun sequence.
ACCESSION   DADMGC010000098 DADMGC010000000
VERSION     DADMGC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.098"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..347
                     /locus_tag="JNZ73_002954"
     CDS             <1..347
                     /locus_tag="JNZ73_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HAY8733169.1"
                     /translation="QVFFAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHTPASL
                     LTTLTEPQDRSLAINNPQLAADVKTAWLKEDPSLLLFVEQPDLSQLRDLVKTGATRKI
                     RSEARHRLEEKQ"
     gene            344..799
                     /locus_tag="JNZ73_002955"
     CDS             344..799
                     /locus_tag="JNZ73_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733170.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(877..1788)
                     /gene="rdgC"
                     /locus_tag="JNZ73_002956"
     CDS             complement(877..1788)
                     /gene="rdgC"
                     /locus_tag="JNZ73_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HAY8733171.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELHDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            1913..2821
                     /gene="mak"
                     /locus_tag="JNZ73_002957"
     CDS             1913..2821
                     /gene="mak"
                     /locus_tag="JNZ73_002957"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HAY8733172.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            complement(2964..4145)
                     /gene="araJ"
                     /locus_tag="JNZ73_002958"
     CDS             complement(2964..4145)
                     /gene="araJ"
                     /locus_tag="JNZ73_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HAY8733173.1"
                     /translation="MKKVILSLALGTFGLGIAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALFSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(4271..7414)
                     /gene="sbcC"
                     /locus_tag="JNZ73_002959"
     CDS             complement(4271..7414)
                     /gene="sbcC"
                     /locus_tag="JNZ73_002959"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HAY8733174.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTTLQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(7411..8613)
                     /gene="sbcD"
                     /locus_tag="JNZ73_002960"
     CDS             complement(7411..8613)
                     /gene="sbcD"
                     /locus_tag="JNZ73_002960"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HAY8733175.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            8803..9492
                     /gene="phoB"
                     /locus_tag="JNZ73_002961"
     CDS             8803..9492
                     /gene="phoB"
                     /locus_tag="JNZ73_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HAY8733176.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLLGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            9550..10845
                     /gene="phoR"
                     /locus_tag="JNZ73_002962"
     CDS             9550..10845
                     /gene="phoR"
                     /locus_tag="JNZ73_002962"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HAY8733177.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEASPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            11252..12571
                     /gene="brnQ"
                     /locus_tag="JNZ73_002963"
     CDS             11252..12571
                     /gene="brnQ"
                     /locus_tag="JNZ73_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HAY8733178.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            12647..14020
                     /gene="proY"
                     /locus_tag="JNZ73_002964"
     CDS             12647..14020
                     /gene="proY"
                     /locus_tag="JNZ73_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HAY8733179.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            14176..>14955
                     /locus_tag="JNZ73_002965"
     CDS             14176..>14955
                     /locus_tag="JNZ73_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HAY8733180.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWF"
BASE COUNT         3316 a         3802 c         4025 g         3812 t
ORIGIN      
        1 tccaggtatt ttttgcggaa ctggctacga taaaagataa atcagatgat gaatggtggc
       61 gtgcattgct gcaaagtcgc aatgatcatt tgcgccaaac ggcattacgc aacgcacata
      121 ccccggcgtc gttgttaacg acattgactg agcctcagga tcggtcgctg gctattaata
      181 atccacagct ggctgccgat gtgaaaacgg cgtggttaaa agaggatcca tcattactct
      241 tatttgtcga acaacccgat ctttcgcagt tacgtgattt agtgaaaacc ggggcaacgc
      301 ggaaaattcg cagtgaagcg cgtcaccggc ttgaggaaaa acaatgacac aacgtccatg
      361 gagcaagtta cagcgggaga tctatgactt actgacgcca acgatcaacc tgcaaattca
      421 ctgtacccgt tatcccatgc gcagccaaaa tggtggaagt accgatttac cgcgttattg
      481 gatcacgttg gataaaaacg tgatctggga ttacccgaaa gactttattg caggtaacgc
      541 tggagtgcgt aattttcacg gtgaaacttg ctggtatcct tacctgacgg atatttgttc
      601 aatttctgat ttattgcgtg aatatattga taccccaaaa gcggagctgt taacaaaaca
      661 atttaccagc gataaatggg ggttagtgaa tattctgcgt gctgctgaca ggcgaatagg
      721 gatgaggcga ttagatcagt tacggcgtaa aacgcataat attgctgcgc taaaaattat
      781 tgctcgccgt agcgaataat tatatgcctg gtgtggcttc gtacgccgga taagacgcgg
      841 caggcgtcgc atccggcatt aaaggaaaat cagcaattaa cgttgtgctt cgccacctaa
      901 tccttcaatc aggttttgaa ttaacgctgc cagttcacca gtcatcagga tgaaatcggc
      961 atcaaaacgc tgggcgaaat cttcacggtc gatatcttcg ttttgatcgt gcagctcgtc
     1021 gcagaactta agacgcttga gcgaaccatc gtcgcacatc acaaactgaa tgcgctgctg
     1081 ccagtcgagc gccagtttag tcaccacttt tccggcttca atgtgattgg tgatctcttc
     1141 gctggtcaga tcttgtttct tcgcgcggat cacgccgcca tcttccagca acgatttcag
     1201 ctcggcttca tcaagcagct ggaagccctg tgccgcacta ccggagcgaa cccattcggt
     1261 cagcgtcagt tcaatcgggt tttccatgct caatggtaca accggtaacg accccaggct
     1321 tttacgcagt aatgccagcg tatcttccgc ttttttggca ctggcgcagt ccaccataat
     1381 caaaccgtta accgtgtcga gccacatcat tgtctggcta aaacggctga aagcacgcgg
     1441 cagcagagag tgcagcactt cgtctttcag cgaatctttt tcggttttct tcagcttacg
     1501 cgcctgttcc gcttccagtt tggcgatttt cgcttccagc gcctgtttaa tcaccggaga
     1561 cgggaggatt ttttcttctt tgcgcgcgca gataacaatt tgaccattgg caacgtgcgt
     1621 taacgcatcg ctgtgcgatc ccatcggagg aacccagccc atcttcgcca tgtcctggct
     1681 gccgcatggg gtaaatgcca tcgaggctag ctgtttttcc atctcttctg cacgcagcga
     1741 aatctcgcgg ctaagacggt aaaccattaa atttttgaac cacagcatga taatttccac
     1801 ggccttgtcg ttaaatttag cgggcatgat aacgaattgt cggcggcctt gcattgccaa
     1861 tccggttgtc cgtctctacg ctattgatat tgaaaaaaat aaggagagta cagtgcgtat
     1921 aggtatcgat ttaggcggca ccaaaactga agtgattgca ctgggcgatg caggggagca
     1981 gttgtaccgc catcgtctgc ccacgccgcg tgatgattac cggcagacta ttgaaacgat
     2041 cgccacgttg gttgatatgg cggagcaggc gacagggcag cgcggaacgg taggtatggg
     2101 cattccaggt tcgatttcgc cctacaccgg tgtggtgaag aatgccaatt caacctggct
     2161 caacggtcag ccattcgata aagacttaag cgcgaggttg cagcgggaag tgcggctggc
     2221 aaatgatgcc aactgtctgg cggtttcaga agcagtagat ggcgcggcag cgggagcgca
     2281 gacggtattt gccgtgatta tcggcacggg atgcggcgcg ggcgtggcat tcaatgggcg
     2341 gacgcatatc ggcggcaatg gcacggcagg tgagtgggga cacaatccgc taccgtggat
     2401 ggacgaagac gaactgcgtt atcgcgagga agtcccttgt tattgcggta aacaaggttg
     2461 tattgaaacc tttatttcgg gcacgggatt cgcgacggat tatcgtcgtt tgagcggaca
     2521 tgcgctgaaa ggcagtgaaa ttatccgcct ggttgaagaa agcgatccgg tagcggaact
     2581 ggcattgcgt cgctacgagc tgcggctggc aaaatcgctg gcacatgtcg tgaatattct
     2641 cgatccggat gtgattgtcc tggggggcgg gatgagcaat gtagaccgtt tatatcaaac
     2701 ggttgggcag ttgattaaac aatttgtctt cggcggcgaa tgtgaaacgc cggtgagtaa
     2761 ggcgaagcac ggtgattcca gcggcgtacg cggcgctgcg tggttatggc cacaagagta
     2821 aaaaacgtag gcaattggcg catcatgcct gatgcgacgc ttgccgcgtc ttatcaggcc
     2881 tacaaaatgt gccagaaccg cgtagggcgg ataaggcgtt cacgccgcat ccggcaataa
     2941 gcaacgcgca ccgtgacagt aatctacccc agtggtttcg ccagcaccgg actatccgcc
     3001 gcttgctggc gcttatagcg accatacagc agcaacgatg acatcgcagc aaacgaaaac
     3061 agggcggcag gcagtgccac gtaattatat gccagcccca gcgtcagcat catacctccg
     3121 caatatgcgc cgacggcgct accgaggtta aacgctattt gcccacctgc ggcacctaat
     3181 aactctccgc ctttggcatt ttgcagtaac aatatttgca gaggtgctga aagggcaaat
     3241 aatcccgcgc aacaaataaa agcaaaaata agcgacgttg ttttcatgcc gccgcagaaa
     3301 aagagcatca gcagtgccag tacaatgata aagtcagtca ctgctgcaat gcgcagtggt
     3361 gaataacgtc ctgaaatcct gccacttagc atatttccca gcaccatccc tagcccaact
     3421 aacatcataa taaaggtcat cgccgtttcc gaaaaaccgg aaataaacat catgtatggc
     3481 tttacgtagc tgaaccaggc aaacacacct gcgttgccaa acatcgtggc ggcgaaaatt
     3541 aaccacgggg ccgggctgcg caaaaagtga aattgttcgc gcagatttcc tttcgcctcg
     3601 tcgcgaatat ctggcaccca aaaatagacc gatgccatca ccgcaatatt aaaaacagcg
     3661 atcaataaaa aggtgtaacg ccagcattcc tgacttaaat acgttcccag cggaatgccc
     3721 agcaaattgg cgactgtcat cccggaaacc atccccgcca cggcggcggt gacttttccg
     3781 ggtttgataa tttttgataa cacgatcgct ccgacgccaa aaaatgcgcc atgcggaaag
     3841 ccggatacca gccgaccaat ggcgagcatc aggtaagacg aagagagcgt gaacatggcg
     3901 ttgccaatga cgcacaacgc caccagaaac aacaagatat gtttgagtga gtagcggctg
     3961 gaaaagagtg cgatgattgg cgcaccgacc accaccacca gtgcataata cgagatcata
     4021 tgcccggcag caggaatcga aattcctacg ttatgcgcca gctccgtgag cacgctcata
     4081 atgccaaatt cggctatccc caaaccaaac gtgcccagag ccaaagataa aatgactttt
     4141 ttcataccac catcacaacc accagaacgg ctaccaatca ccgtagccaa taaagtgata
     4201 gcttgcagga taacgaccag caaggccaag agataattcg cccctctgta ttcattatcc
     4261 tgctgaatag ttatttcact gcaaacgtac tttccagttt gctgtagccc aggccgttga
     4321 tctttttcac tttgatctgc accggaatac gctctttcat cgcttctacg tggctaatca
     4381 caccgatggt tttgccactg gcgttcaggg catccagcgc atcaagggcg gtatccagcg
     4441 tttcgctatc cagcgtgcca aaaccttcat caaggaacag cgagtcaata cgtgttttat
     4501 ggctgaccag atccgaaagc gccagcgcca gcgccagact aacgaggaaa ctttcgccgc
     4561 cggaaagggt acgggtatcg cgtaccgcat ctgcctgcca ggtatcaaca acctcgactt
     4621 ccagcgcctc gctggctttg cgctgtaaca gatagcgccc gtgcagccgg gtaagttgct
     4681 gattagcgag atggactaaa ttatccagcg tcagcccctg ggcaaacttg cggaatttat
     4741 cgccctcttt ggaacctatt agcgaattca gatatcccca gtcctcaacc tgctgcgtca
     4801 tttgagcaat ttgctgcatt aaggtttgtt gttgctgacg gttatctgca tcctgcttca
     4861 gctgctggcg aatctcgcct tgactcgtgg tgttttcacg caacttttgg tgagtttgcg
     4921 ctaactcttg ctgaatctgc tccaccgtca cagtgagagc caacccgtca ggtcggtgtt
     4981 gttgatgctg tgccagcgtt tctgctgtct gagtgaccag cgtttgcgcc tgacggcgct
     5041 ggttttccag attctgcttg agctgttcca gctgcgttag tgtttgttca tccattagcg
     5101 ccgcaaggaa cgcctgctga tcgtcaaaga cgctggcctg tagcgtggtg tcaaactgcg
     5161 cttgggcttt ttgcagactt tgcgccgcca gaacatcctg ttgctgtaac gtctgctgct
     5221 ggctgtgtaa tgcgagacat tgttcatgaa cctgccgcca gttatccagc gccacagttt
     5281 cttcgctgtg cgggagatca tcactttgcg gcaacgtttc cagaatcggc gtcagctgct
     5341 gaatacggtt ttgcagcgcg gttaattcgt tctggcgttg ctgccagctc tgcgcttctt
     5401 gctgacgtgt cgccaaccag ctctcttctt catcttcctg tggcaatgtc agtgcataac
     5461 ccgccaatgc cgttaagagc tgctgctgac gttgttcaat ttgctgttga tactggataa
     5521 tttgctgatt atgcgcggca atctgccctt gtaattcatg tcgttggctg agtaaccgca
     5581 gctggcgttc gtgctcatct tgtgcatcca gccacggttg aatatcgtcc tgtggctgca
     5641 aggtgatatt gaggctggcc gtgacggctt gccattgttg agtaagtgct tgctcatctt
     5701 gtcggaggct ttgcgcttcg ttttcatcgc gctgaagctg cttcgttaat gcatccagtt
     5761 gcccgcgcag cgccgcacct tcttcgccga gctttttaac ttcgttttcc agcgccagta
     5821 atcgagactg attaacacca ggctccagcg cctgatacgc ctcgaccgcc gggtggctgg
     5881 tggaaccaca aagtggacaa ggctgacccg cctgtaactg tgcacgctgg gcttccagcg
     5941 ttttgatgcg cgcttcctgc tcgcaaatgg ttttcacatc ggcaagttgc tgcgtctttt
     6001 ctttataacg ctggcgcatt tcgttaagtg cgacattacg ttgcgtctgt tccagagtga
     6061 cattctggat agtgaccatt aactgcgcca gacgtttttg ttggggaaca atctgcccat
     6121 gcagcgcgac caggcgctga cgcagtgggc gttgctcggc atgttgcgcc agggcggtag
     6181 caacttcatc ggcggttaac gtcaacgtga tcgccgcaag cgcattaagt ttttgctcag
     6241 catgggttaa ctgttgctgc cattgccgca gatgctcgcg atcgctggtt tgttgggaga
     6301 actgcgcacg ccaacccgcc agttcgttgt tccactgacg gaagcggtcg tgttcctgta
     6361 accaggtatt caggctttgt tgctgctgct gtaattctgc tgactgcttc gccgcgtggt
     6421 ggcgaatgct cgcgcgaagc gccattgtgc tctgtaagcg agtatttact tcttcaatct
     6481 gctggcgaat atgcgccagc gccgcgctgt gttctgcgat gcgttcccag tgtggacgaa
     6541 gatttcgtgc cggttgtgcc agactaagcg ccgccagttg aggttgcgct ttttcttctt
     6601 cggctaacgc ctgttgcaag gcctgctgac ggcggctggc ttcttgctgc aattcgtcct
     6661 gacgcgttaa ccagtttagc gattgttgtt cttgctgctg cgcggtaatt aactgttttt
     6721 cttcgtcagt aagtacctgc aaactcgctg tcagcgattg cacttgttcc ggcgtgagca
     6781 acgtgacgcc gctggcctgc gcttgcagct tctccagctc agtacgggcc gatttgtgct
     6841 gctcaaaaac catcgccgag atttgcccgt agatttcagt gccggttaac tcctcgagca
     6901 attccgcgcg ttctttgggt ttggcattca ggaaggcagc aaattgcccc tgcgaaagca
     6961 gcatcgaacg ggtgaagcgc ccgtaatcca gcccggttaa cgtcgctgtc agttccagct
     7021 tatctttcac tttgtcggcg agaattttgc cgtcggcgca gcgcgccagc tctacgcgag
     7081 gcacctgcaa attaccgtcc ggctggttac gcgcccgatt ctggctccag aacgcgcgat
     7141 acgcttcacc tttcacttca aactccacct ccgccagaca ttcagcggta tcgcgggtca
     7201 tgagatcatt ttgcgattgt gaaacgttag agagacgcgg agtttcgtga tacagcgcca
     7261 gacaaatggc gtccagcagg gtggttttcc ccgcaccggt tgggccggta atggcaaaca
     7321 gcccgttgct ggcgaacggc tcgcgggtga aatcaatctt ccattcgcct tttaatgagt
     7381 tcaggttttt caggcgcagg ctgagaattt tcatgcttcg tgttctccgg cgagggtatg
     7441 caacgtcgtg gtgaaaagat gctgcagacg ttgctgctgc gattcatcca gttcttccag
     7501 tgccagacgg cgattgaaca cctcttcgac gctgagttcg ctgagggttt cacgctgttg
     7561 gctggctaac acacgctcgc gctgttcacg actccgacgt accagcaata cttcgacagg
     7621 caatgattcg gttaatgcct ggattttgcg ctgaatatca tgcagatact catcagtagt
     7681 gatttcgata tccagccaga caggtggctc ctgcgataca tcgcgccact gttccagctg
     7741 tgcggtaatc gacgccagat cgcctttcag caccgccatg ggttgagtta ccggtacgtt
     7801 caggttttcc acgctctcta atttgccgtt tgaaaatgtc accagatgga catatttact
     7861 cttaccgcat tcatcaaaac tcagtggaat gggggaaccg caatagcgaa catgttccat
     7921 gccgccaata atctgtgcgc ggtgaatatg cccgagcgcg atgtagtcgg ctggtggaaa
     7981 gttttgtgcc ggaaacgcgt ccagcgtgcc aatataaatg tcacgcacgg cgtcactttt
     8041 actggcaccc acggtcgtta aatgtcccgt ggcgatgatg ggcagaggct gatcgccgcg
     8101 cagtttgcag gcatcggcat agtgttgttg gtaataatcg gtaatcgctg ccagtaaatg
     8161 ctgctgtttt tcaataccgt taagccccgc ctggctggaa ataatgtcac gcgggcgtaa
     8221 aaacggaatg gggcacagca ctgcgcctgg cgtcccgtcg cgacgaggca agatttgcgg
     8281 cgcatgtccg gcgctggcga ccacggttgt attgaggaac gccatgatat cgcgcgattc
     8341 attcagcgtg gcgaccgagt catggtttcc tgccagtacc accagatgac agccagtttg
     8401 ctgtaaattg acaacaaaac ggttgtataa cgtgcgggcg taactgggcg gcgagccggt
     8461 atcgaaaaca tcaccagcaa caataatcgc atccacctga tgggtttgtg ctgtctccag
     8521 cagccagtca agaaaagcct gatgttcagc ttcgcggctt ttactgtaga agttctggcc
     8581 gagatgccag tctgaggtgt gaaggatgcg cataacggtt ccctggcgaa aaagcatggt
     8641 cgcgattata cccaaacaga tgtgccattt gcttttttct gcgccacgga aatcaataac
     8701 ctgaagatat gtgcgacgag cttttcataa atctgtcata aatctgacgc ataatgacgt
     8761 cgcattaatg atcgcaacct atttattaca acagggcaaa tcatggcgag acgtattctg
     8821 gtcgtagaag atgaagctcc aattcgcgaa atggtctgct tcgtgctcga acaaaatggc
     8881 tttcagccgg tcgaagcgga agattatgac agtgctgtga atcaactgaa tgaaccctgg
     8941 ccggatttaa ttctccttga ctggatgtta cttggcggct ccggtatcca gttcatcaaa
     9001 cacctcaagc gcgagtcgat gacccgggat attccagtgg tgatgttgac cgccagaggg
     9061 gaagaagaag atcgcgtgcg cggccttgaa accggcgcgg atgactatat caccaagcca
     9121 ttttcgccga aggagctggt ggcgcgaatc aaagcggtaa tgcgccgtat ttcgccaatg
     9181 gcggtggaag aggtgattga gatgcaggga ttaagtctcg acccgacatc tcaccgagtg
     9241 atggcgggcg aagagccgct ggagatgggg ccgacagaat ttaaactgct gcactttttt
     9301 atgacgcatc ctgagcgcgt gtacagccgc gagcagctgt taaaccacgt ctggggaact
     9361 aacgtgtatg tggaagaccg cacggtcgat gtccacattc gtcgcctgcg taaagcactg
     9421 gagcccggcg ggcatgaccg catggtgcag accgtgcgcg gtacaggata tcgtttttca
     9481 acccgctttt aacgccttgc tcatcggacg cagagcaggg cttatgattt cttaactgga
     9541 gtatcttacg tgctggaacg gctgtcgtgg aaaaggctgg tgctggagct gctactttgc
     9601 tgcctcccgg ctttcatcct gggtgcattt tttggttacc tgccctggtt tttgctggca
     9661 tcggtaacag gactgcttat ctggcatttc tggaatttat tgcgcctttc atggtggctg
     9721 tgggtggatc gcagtatgac cccgccaccg gggcgtggta gctgggaacc gctactatac
     9781 ggcttacacc agatgcagct gcgaaataaa aaacgccgcc gtgaactggg caatctgatt
     9841 aaacgctttc gtagcggcgc ggagtcgctg cccgacgcgg tggtgctgac cacggaagag
     9901 ggcggtattt tctggtgtaa cggtctggcg caacaaattc ttggtttgcg ctggccggaa
     9961 gataacgggc agaacatcct taacctactg cgttacccgg agtttacgca atatctgaaa
    10021 acgcgtgatt tttctcgccc gctcaatctg gtgctcaaca ccgggcggca tctggaaatt
    10081 cgcgtcatgc cttataccca caaacagttg ttgatggtgg cgcgtgatgt cacgcaaatg
    10141 catcaactgg aaggggcgcg gcgtaacttt tttgccaacg tgagccatga gttacgtacg
    10201 ccattgaccg tgttacaggg ttacctggag atgatggatg agcagccgct ggaaggcgcg
    10261 gtacgcgaaa aagcgttgca caccatgcgc gagcagaccc agcggatgga agggctggtg
    10321 aagcagttgc taacgctgtc gaaaattgaa gcctcgccga cgcaattgct caatgaaaag
    10381 gttgatgtgc cgatgatgct gcgcgttgtt gagcgcgagg ctcagactct gagtcagaaa
    10441 aaacagacat ttacctttga gatagataac ggcctcaagg tgtctggcaa cgaagatcag
    10501 ctacgcagtg cgatttcgaa cctggtctat aacgccgtga atcatacgcc ggaaggcacg
    10561 catatcaccg tacgctggca gcgagtgccg cacggtgccg aatttagcgt tgaagataac
    10621 ggaccgggca ttgcaccgga gcatattccg cgcctgaccg agcgttttta tcgcgttgat
    10681 aaagcgcgtt cccggcaaac cggcggtagc ggattagggt tagcgatcgt gaaacatgcg
    10741 gtgaatcatc acgaaagtcg cctgaatatt gagagtacag taggaaaagg aacacgtttc
    10801 agttttgtta tcccggaacg tttaattgcc aaaaacagcg attaatccgc ctttgtcatc
    10861 ttttattgcc ataagccagc cgatgctggc ttattttctt tgcagtcaaa atacgggcgt
    10921 tagattttac aacggttggt gattttttgt tcgcatgatt agccatgtct ttttcacgga
    10981 aatagtgttt tatactggtt ggtgatttct tatcgctata tacctctggt ttttagatcc
    11041 ctccttgctt taaaacgtta taagcgttta aattgcgctt caggtgctgt catactgact
    11101 gcattaacgc ggtaaatcga aaaactattc ttcgccgcgc ctggttggga gtattttccg
    11161 ctaaaattgt ttaaatatac cgctgtatca tccccaggga ttggcacaaa aatttaacgt
    11221 tacaacacca catccacagg cagtatgatt tatgacccat caattaagat cgcgcgatat
    11281 catcgctctg ggctttatga catttgcgtt gttcgtcggc gcaggtaaca ttattttccc
    11341 tccaatggtc ggcttacagg caggcgaaca cgtctggact gcggcattcg gcttcctcat
    11401 tactgccgtt ggcctgccgg tattaacggt agtggcgctg gcaaaagttg gcggcggtgt
    11461 tgacagcctc agcacgccaa tcggtaaagt cgctggcgta ctgctggcaa cggtttgtta
    11521 cctggcggtg gggccgcttt tcgctacgcc gcgtacagct accgtttcct ttgaagtggg
    11581 gattgcgccg ctgacgggtg attccgcgct gccgctgttt atctacagcc tggtctattt
    11641 cgctatcgtt attctggttt cgctctatcc gggcaagctg ctggataccg tgggcaactt
    11701 ccttgcgccg ctgaaaatta tcgcgctggt catcctgtct gttgtcgcga ttgtctggcc
    11761 ggcgggttct atcagcacgg cgactgaggc ttatcaaaac gctgcatttt ctaacggctt
    11821 cgttaacggc tatctgacca tggatacgct gggcgcaatg gtgtttggta tcgttattgt
    11881 taacgcggcg cgttctcgtg gcgttaccga agcgcgtctg ctgacccgtt ataccgtctg
    11941 ggctggcctg atggcgggtg ttggtctgac tttgctgtac ctggcgctgt tctgtctggg
    12001 ttcagacagc gcgtcgctgg tcgatcagtc tgcaaacggc gctgctattc tgcatgctta
    12061 cgttcagcac acctttggcg gcggcggtag cttcctgctg gcggcgttaa tcttcatcgc
    12121 ctgtctggtc acggcggttg gcctgacctg tgcttgtgca gaattctttg cccagtacgt
    12181 accgctctct tatcgtacgc tggtgtttat cctcggcggc ttctcgatgg tggtgtctaa
    12241 cctcggctta agccagctga tccagatctc cgtaccggtg ctgaccgcta tttatccgcc
    12301 gtgtatcgca ctggttgtat taagttttac acgctcatgg tggcataatt cgtcccgcgt
    12361 gattgctccg ccgatgttta tcagcctgct ttttggtatt ctcgacggga tcaaagcatc
    12421 tgcattcagc gatatcttac cgtcctgggc gcagcgttta ccgctggccg aacaaggtct
    12481 ggcgtggtta atgccaacag tggtgatggt ggttctggcc attatctggg atcgcgcggc
    12541 aggtcgtcag gtgacctcca gcgctcacta aatcactgaa catttgtttt aaccacgggg
    12601 ctgcgatgcc ccgtggtttt ttattgtgtt gatgggttag gaattgatgg aaagtaagaa
    12661 taagctaaag cgtgggctaa gtacccgcca catacgcttt atggcactgg gttcagcaat
    12721 tggcaccggg ctgttttacg gttcggcaga cgccatcaaa atggccggtc cgagcgtgtt
    12781 gttggcctat attatcggtg gtatcgcggc gtatatcatt atgcgtgcgc tgggggaaat
    12841 gtcggtacat aacccggccg ccagctcttt ctcgcgttat gcgcaggaaa accttggccc
    12901 gctggcaggt tacattaccg gatggaccta ctgctttgaa atccttattg tcgccatcgc
    12961 cgatgtgacc gcttttggta tctatatggg ggtcttgttc ccgacggtgc cgcactggat
    13021 ttgggtactg agcgtggtgc tgatcatttg cgccgtaaac ctgatgagcg tgaaggtctt
    13081 cggtgagctg gaattctggt tctcgttctt taaagtcgcc accatcatca tcatgattgt
    13141 cgccggtttc ggcatcatca tctgggggat tggcaacggc gggcaaccga ccggtattca
    13201 taacctgtgg agcaacggcg gcttcttcag taacggctgg cttggcatgg taatgtcgtt
    13261 gcaaatggtg atgtttgctt acggtgggat cgaaattatc gggattaccg ccggtgaagc
    13321 gaaagatcct gagaaatcga ttccgcgtgc gattaactcc gtgccgatgc gtattctggt
    13381 gttctacgtc ggtacgctgt tcgtcattat gtctatctac ccgtggaatc aggttggcac
    13441 tgccggtagc ccgttcgtgc tgacgttcca gcatatgggc attacctttg ccgccagcat
    13501 tcttaacttt gttgtgctga ccgcttcgct gtcggcaatt aacagtgacg tatttggcgt
    13561 aggccgtatg ctccacggta tggcagagca gggcagcgca ccgaaaattt tcagcaaaac
    13621 gtcgcgtcgc ggtattccgt gggttacggt gctggtgatg actaccgcgc tgctgtttgc
    13681 ggtgtatctg aactacatca tgccggaaaa cgtcttcctg gtgatcgctt cgctggcaac
    13741 cttcgccacg gtatgggtgt ggattatgat cctgctgtcg caaatcgcct tccgtcgccg
    13801 tttgccgcca gaagaagtta aggcgctgaa atttaaagtg ccgggtgggg tagcaacgac
    13861 catcggcggg ctgattttcc tgctctttat tatcgggttg attggttatc acccggatac
    13921 gcgtatctcg ctgtatgtcg gtttcgcgtg gattgttgtg ctgttgattg gctggatgtt
    13981 taaacgccgc cacgatcgtc agctggctga aaaccaataa tacttgcgtt ctgtaagccg
    14041 gttcaggagg aaatcctgat ccggctattc cgaaagttat ccgcccccgt cctcctcccc
    14101 caaatatcct tcagatgatg agtgatcctg cattaggcta tggcaaggtg atcagatttt
    14161 catcacaggg gaattatgat gttaaatgca tggcacctgc cggtgccccc atttgttaaa
    14221 caaagcaaag atcaactgct cattacactg tggctgacgg gcgaagaccc accgcagcgc
    14281 attatgctgc gtacagaaca cgataacgaa gaaatgtcag taccaatgca taagcagcgc
    14341 agtcagccgc agccaggcgt caccgcatgg cgtgcggcga ttgatctctc cagcggacaa
    14401 ccccggcggc gttacagttt caaactgctg tggcacgatc gccagcgttg gtttacaccg
    14461 cagggcttca gccgaatgcc gccggcacga ctggagcagt ttgccgtcga tgtaccggat
    14521 atcggcccac aatgggctgc ggatcagatt ttttatcaga tcttccctga tcgttttgcg
    14581 cgtagtcttc ctcgtgaagc tgaacaggat catgtctatt accatcatgc agccggacaa
    14641 gagatcatct tgcgtgactg ggatgaaccg gtcacggcgc aggcgggcgg atcaacgttc
    14701 tatggcggcg atctggacgg gataagcgaa aaactgccgt atctgaaaaa gcttggcgtg
    14761 acagcgctgt atctcaatcc ggtgtttaaa gctcccagcg tacataaata cgataccgag
    14821 gattatcgcc atgtcgatcc gcagtttggc ggtgatgggg cgttgctgcg tttgcgacac
    14881 aatacgcagc agctgggaat gcggctggtg ctggacggcg tgtttaacca cagtggcgat
    14941 tcccatgcct ggttt
//
LOCUS       DADMGC010000099        14928 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.099, whole genome shotgun sequence.
ACCESSION   DADMGC010000099 DADMGC010000000
VERSION     DADMGC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.099"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            84..365
                     /locus_tag="JNZ73_002966"
     CDS             84..365
                     /locus_tag="JNZ73_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733181.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVIAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHGKKPERLRR"
     gene            complement(1162..2622)
                     /gene="phoQ"
                     /locus_tag="JNZ73_002967"
     CDS             complement(1162..2622)
                     /gene="phoQ"
                     /locus_tag="JNZ73_002967"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HAY8733182.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(2622..3293)
                     /gene="phoP"
                     /locus_tag="JNZ73_002968"
     CDS             complement(2622..3293)
                     /gene="phoP"
                     /locus_tag="JNZ73_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HAY8733183.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(3462..4832)
                     /gene="purB"
                     /locus_tag="JNZ73_002969"
     CDS             complement(3462..4832)
                     /gene="purB"
                     /locus_tag="JNZ73_002969"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAY8733184.1"
                     /translation="MELSSLTAVSPVDGRYGDKLSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(4836..5477)
                     /gene="hflD"
                     /locus_tag="JNZ73_002970"
     CDS             complement(4836..5477)
                     /gene="hflD"
                     /locus_tag="JNZ73_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HAY8733185.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(5513..6619)
                     /gene="mnmA"
                     /locus_tag="JNZ73_002971"
     CDS             complement(5513..6619)
                     /gene="mnmA"
                     /locus_tag="JNZ73_002971"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAY8733186.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGHEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(6673..7134)
                     /gene="nudJ"
                     /locus_tag="JNZ73_002972"
     CDS             complement(6673..7134)
                     /gene="nudJ"
                     /locus_tag="JNZ73_002972"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HAY8733187.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(7144..7797)
                     /gene="rluE"
                     /locus_tag="JNZ73_002973"
     CDS             complement(7144..7797)
                     /gene="rluE"
                     /locus_tag="JNZ73_002973"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HAY8733188.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            7969..9219
                     /gene="icd"
                     /locus_tag="JNZ73_002974"
     CDS             7969..9219
                     /gene="icd"
                     /locus_tag="JNZ73_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HAY8733189.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(9322..9645)
                     /gene="iraM"
                     /locus_tag="JNZ73_002975"
     CDS             complement(9322..9645)
                     /gene="iraM"
                     /locus_tag="JNZ73_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY8733190.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGRIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESEITNKCLHKSCIIKIC
                     PYGLK"
     gene            complement(10178..10288)
                     /locus_tag="JNZ73_002976"
     CDS             complement(10178..10288)
                     /locus_tag="JNZ73_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733191.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQAHTIKLIFEM"
     gene            complement(10341..10745)
                     /locus_tag="JNZ73_002977"
     CDS             complement(10341..10745)
                     /locus_tag="JNZ73_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HAY8733192.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNQLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(10966..11697)
                     /locus_tag="JNZ73_002978"
     CDS             complement(10966..11697)
                     /locus_tag="JNZ73_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY8733193.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRHYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFETRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            complement(11902..13113)
                     /gene="bluF"
                     /locus_tag="JNZ73_002979"
     CDS             complement(11902..13113)
                     /gene="bluF"
                     /locus_tag="JNZ73_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HAY8733194.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMTHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            13427..13663
                     /locus_tag="JNZ73_002980"
     CDS             13427..13663
                     /locus_tag="JNZ73_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HAY8733195.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSHCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            13706..13978
                     /locus_tag="JNZ73_002981"
     CDS             13706..13978
                     /locus_tag="JNZ73_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HAY8733196.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            14007..14273
                     /gene="ariR"
                     /locus_tag="JNZ73_002982"
     CDS             14007..14273
                     /gene="ariR"
                     /locus_tag="JNZ73_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HAY8733197.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            14386..14634
                     /locus_tag="JNZ73_002983"
     CDS             14386..14634
                     /locus_tag="JNZ73_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733198.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         4005 a         3400 c         3479 g         4044 t
ORIGIN      
        1 gcatgaggat tttgcttttg atataagcga cagccaccgc cagaaactgg aagaatttag
       61 cggggtgtaa tgaggtcagc agcatgacag aagccgaaat aacgggatta atccgccgcg
      121 ttgccggaat cagccagcag gctgacgaac aggccacgca gccggacagc gtgatagccg
      181 aaaactatgc gcgtgttgtt gctgaggtga tgcgccgtga tggtatagaa cttaatggcg
      241 tggatatgcg caacatacga accagagttc ttgagttgct ggcctaccgt cgccgcgtgg
      301 agatgtatcg ggagaaggag aaaatcacgt accacgggaa gaagccggag cggttacggc
      361 ggtaactcac tgagattaaa gcaaagcgca aaattgcgct ggctacatcc ctcacatctg
      421 aggactgtta accagcaggc cggaatatat tcagaaacta cgaaattttc gcagtttaat
      481 tactcgtcag cttaatcgct aacccgctct gatattttcg gaaacctcaa tttgaggaag
      541 tcggcgaggt aactgtttga tattcccgat agcgcaaaaa tgcgcggtcg ggcagaatga
      601 ccaccacctc aatcgtgacg gagagtgaca accagtgaca caccgtgaca aaaagacggt
      661 ttaaccgttc attaaaaatc gacgtgattt tgtgctttcc ggatgacgtg gccaacgtca
      721 ttttttagcg aaaaattctg attaaggtca ctggctgaac ggtcataatg accacgcaga
      781 ccatcgaggc aaccatagcg atcatcgtta cggttggagc ttttccgcca ttggtggaaa
      841 aggtgcacat ctggatcatc attacggtga tcaagggtat gtaaaaccca ccagcctgat
      901 taaaggttaa ccggaaaaaa aatccggtag ccccgttatg gggatcccca tatcgacatt
      961 aacgcccctc atgaattggt gcgcttcccc ctggaaagat tacccgcctg tatatttctt
     1021 gtgtctattt gttccacgtt gtttcataca gtgcaccgaa cggtgtagtt aatggtgtag
     1081 tcaatttgcg atttttggca ctttttcaat gaaaattgtt taatcgaatc aatgacttga
     1141 tgtagtggta aaaggacata tttattcatc tttcggcgca gaatgctggc gaccaaaaat
     1201 cacctccatc cgcgcaccgc ccagcatgct ctctccggcg acgattttac cctcatattg
     1261 ctcggtgatt tcgcgggcta ccgccagccc tacaccttgc ccagggcgta aagtatcaac
     1321 ccgttgacca cggtcgaaaa tgacctctcg cttgcttaat ggaataccgg ggccatcatc
     1381 ctcgaccaca atatagagat gctcgtcggt ttgccttgca gaaatttcga caaactcgag
     1441 gcaatattta caggcattat ccagcacgtt gcccatcacc tcgacaaaat cgttctgctc
     1501 accgacaaag ctgatctctg gcgaaatatc gagagagata ttgacccctt tgcgttgata
     1561 cactttgttc agcgctgagg tgagattgtc cagcagtggg gcgaccggat gcagctcgcg
     1621 gctgagcaat gtcccgccgc gcatactggc acgatgcagg tagtagccaa tttgctgtga
     1681 aatgcggctg atttgctcca gcattaccgg ctcagcatca ctgacgctca tcttttcact
     1741 acgcagagaa cgcagcgtac tttgcagcac cgccagtggc gttttcagac tatgggtcag
     1801 gtcggtgagc gtcgtacggt atttgtcgta acgttcgcgt tcacttttta acaatcggtt
     1861 caggtttcgt accagactgg tcagttctcg cgttgtggct ggattgagca attcgcggtt
     1921 atgttcttcc agttcgcgga cttcttttgc cagggcttcg atggggcgta aactccacca
     1981 ggcggcgacc cacagcagcg ggatcactaa cagcagattg gctgagagta catagataaa
     2041 ccagctccag accatatagg aactttttag ctccaccgga atggtatcca ccaccacaat
     2101 ggttaatttt ggcatccgcg atgttgccgg gtagacgttt accgccaccg agtgggtcat
     2161 ctccgcgtcg tcatcatctt cccgcacttc ctgcaactgt tgctgtatcg aatgatctcc
     2221 actcagcaag aggctggtat cgttaacatc cgcttcaatt tcatgaaaac cattcgattt
     2281 cagccagtca ggctggatca ttttcatcag ccagggcacg tcacgttgcg cccataaaag
     2341 ctgcccgttc tcatcataaa ttagcgtcat ggtggggctt tgcttgtcga tattttcggg
     2401 taactcgaca tgcaacttat tgttttccca ctgcgcaagg gtatagaaca gattgctctc
     2461 gccacgtaac agccgaaacg tagttttatc gaaactgacg ctataaccga tcagcgcgac
     2521 cattccgtag gcaagcgaaa gcaccagtac taccgctgcc gttgccaaca gaaaacgtac
     2581 ccgcagcgag agcgggaaaa aaagacgcag taattttttc atcagcgcaa ttcgaacagg
     2641 tagccctggc cgcgaacggt ggtaatgact tcttgcggat attgtgcctg aatttttttg
     2701 cgcagacgtc ccatcagtac atcaatggta tggctttccc gcagctccgc atccggatag
     2761 agttgtagca ttaacgaatc tttgctgacc actttgccat tattgcgtat caacgtttcc
     2821 ataatggtgt attcgaacgc ggtcagtttg atcacttcgt cattaataga taattcacgg
     2881 cgagagagat caacctgaaa cggggggagc gaaatgacct gtgaagccag accgctatta
     2941 cgccgcatta atgcctgcat tcgcgccatc acctcttcaa tatgaaacgg tttagtcacg
     3001 taatcatcag caccggcact taatacttcg actttgtcct gccagctttc acgggcggtt
     3061 aataccagaa tcggcagtga aacatcgttg ctacgccagc ggcgaatcag tgacagaccg
     3121 tcctcgtcag gcaatccgag atcgacaatc gcaatatccg gtaaatgttc attgagataa
     3181 taatcggctt ctttggcatc ttctgcgtca tcgacctgat gaccagcatc ctgaatctga
     3241 actttaaggt ggtgacgtaa caacgcattg tcttcaacaa ccagtacgcg catttttatt
     3301 tctccctgtc ttaattatta aaatagtgta acgcgattat gtgtttatgg gggtaaacat
     3361 taaataaacc agcggggagg ggaggtaaag tgaaaaaaga aaaagtggat aatcttaata
     3421 agcaggccgg acagcatcgc catccggcac tgatttgagg cttatttcag ctcatcaacc
     3481 atcgtgatag ctcgaccaat atagttagcc ggcgtcatcg ctttcaggcg ggctttctct
     3541 tcttctggca acgccagacc atcgataaac tgcttcatgc cttcggcgtc aacgcgctta
     3601 ccgcgagtaa gctctttcag cttctcgtac ggtttttcga tgccatagcg acgcataact
     3661 gtctggattg gttctgccag cacttcccag ttgtgatcca gctcatccag cagatggtca
     3721 cggttcactt ccagtttgct cacgcctttc agggtggatt gatacgcaat cagcgcataa
     3781 ccgataccca cgccgaggtt acgcagcacg gtggagtcgg tcaggtcacg ctgccagcgg
     3841 gaaaccggca gtttgcttgc cagatgctgc aataccgcgt tggaaaggcc cagattccct
     3901 tcggagtttt cgaagtcgat cgggttaact ttatgcggca tggtggaaga accaatctca
     3961 ccagcaatgg ttttctgttt gaagtggtta agggcgatat aaccccagac gtcacggtca
     4021 aagtcgatca gaatggtgtt gaagcgcgca acgcaatcaa acagttcggc aatgtagtcg
     4081 tgcggttcaa tctgggtagt gtacgggttc cattgaatac ccagcgaggt gacgaactct
     4141 tcgctgaact gatgccagtc aacttccggg taagcggcga tgtgggcgtt atagttaccg
     4201 accgcgccgt tgattttgcc gaggatctcc acctggttaa gctggcggtg ctggcgctcc
     4261 atacggtagg cgacgtttgc catctcttta ccgatggttg acggcgtggc tggctgaccg
     4321 tgggtacgag acagcagcgg gatatcgcgg tactgaacgg cgagatcttt aatgccatca
     4381 atcagttgac gccagtacgg caggatcact tcatcacgcg cggttttcag cattaatgcg
     4441 tgggagaggt tattgatatc ttccgaagta caggcaaagt ggatgaattc agaaaccgcg
     4501 tgcagttccg ggatctccgc cactttttct ttcaggaaat actcaaccgc tttaacgtcg
     4561 tggttagtgg tacgctcgat ggttttgatg cgtgcggcat cttcttcgct gaaattggcg
     4621 acaattgcat caaggtaacc gattgcgtcg gcagcaaaag caggaacttc cttgatcgct
     4681 gcgtgcgcgg ccagtttttg cagccaacgt acttcaactt gtacacggaa tttcagcaaa
     4741 ccatattcgc tgaaaatccc gcgcagcgcg ctgagtttat cgccgtagcg tccatcgaca
     4801 ggggaaacgg cggtcagtga ggataattcc atagatcaca actccggggt taaatgagca
     4861 agaatttgtt ttgcctgagt ggtcaggcga ttacgagaaa acatcagttg tagacgtccg
     4921 ccgccgacct ggtgccagag cacggcggcg cgaatgcctg ccagcagggt tgcgcgaact
     4981 ttcgcctgca cttgtgggct ttgcagtaca gcaggggaac cggtgacctg aatgcgcggg
     5041 ccaagcgggc tgatcacatc aacatagata gcagccatcg cgctcatcag cgtttcggac
     5101 tgtaaatcga agtgttcgag ctggcgttgc aggccgttga tccggttgcc cagagtgtcg
     5161 agcgcgcctt tcgctgagga gagtttgcgc tcaagcacca tcaagctgag tgtgtagcgg
     5221 gttaattcgg cgtttaagcc ctggcaactg ctggcattga gcacgccgag cagggtttcc
     5281 agcccgacgc gcaggttggc ttcgctaccg ccaaaaaccg ccagcgtcga gctggggttc
     5341 atatcaataa tactgttgag tgagacgtgt agcgcatcgg catcacaatg cccctggtga
     5401 gcgagttgtt gcaccaggcg tgccgactga caaataccgg ccagggcgag ggtgatgtca
     5461 tagtaattct ttgccacgtt cactgcttcc ttgttttaag taaagataat aatcagaccg
     5521 gcagcggcag acgctgctca ataataccgc caccgaggca cacttcaccg ttatagaaga
     5581 cggcagactg gcccggcgtc acggcggcaa ccggctcatc gaaaatcact ttaatgcgat
     5641 cgtcgtccag cgccttgacg gtgcacggaa tgtcggtctg gcgatagcgg gtttttaccg
     5701 tgcaacgcat agtgccggtg aatggttcgc gatcgaccca gtgcagctgc tgggcaatca
     5761 acccgacaga catcagccgc gggtgttcat ggccctgagc gacaaccaga atgttgtttt
     5821 cgacgtcttt gtccaccaca taccacggtt cttcggtgcc ttctttggtg ccaccgatac
     5881 ccagaccttt acgctgaccg agagtgtgat acatcagccc ctggtgctca ccaatttcat
     5941 ggccatcgac ggtaatgatt ttgcccggtt gcgccgggag ataacggccc aggaactcgc
     6001 ggaatttacg ttcgccaatg aagcaaatgc cggtagagtc ttttttcttc gcggtgacca
     6061 gaccaagatc ttcagcaatc ttacgcacct gcggtttttc cagttcgccg accgggaaca
     6121 ggctttgcgc aatctgctca tggctgagcg tataaaggaa gtagctctgg tctttattgc
     6181 tgtccagacc acgcagcagg cggctcttgc catcgacatc ggcccgacgt acgtaatgac
     6241 cggtagcgat ataatcggca cctaaatctt cggcggcaaa ttcgaggaag gctttaaatt
     6301 tgatctcctt gttgcacaga atatccggat tcggcgtgcg accggcttta tattcggcaa
     6361 ggaacagttc gaagacgttg tcccagtact cggcagcaaa gttaacggtg tgcagttcaa
     6421 tgccgagctt gtcgcagacc gcctgggcat cagccagatc cgctgccgct gtgcaatatt
     6481 cctcaccgtc gtcttcttcc cagttcttca taaacaggcc ttcgacctga tatccctgtt
     6541 gttgcaacag ccaagcagaa acggaggaat cgacaccgcc ggacatgccg acgattactt
     6601 tttttgcggt ttcagacatt ggatcactca cgacattgaa cttcaaggcg gcgtattcta
     6661 tcacgcagcc ttttagatga caccctttgt aaaaggccag ttaaaatcgc caatcatctc
     6721 cagcggataa cgttgcccgc tttgataaca acgaatactt tccgccacca gcggcgaacg
     6781 aagatttgac gcctgtaaaa tttcttcggc gctgacccaa cggcagcagt cgatatcgct
     6841 gtcatgaggc tgggtcgggc atatttgctc aagctcaatg gcaaagagga aacgcaaaaa
     6901 cggcgtttta tctggcgcaa tccactgatg catacgaata aagtgttgcg gctgcgcgct
     6961 gatgccggtt tcttcccaca gttcacgggc ggcggcttcc actaaggttt catcggcttc
     7021 cagatgcccg gcaggttggt tccataacgc tttaccatta atcgtctctt caacgactaa
     7081 aaatttgcct tctgcgtgca ccacgcaagc aacggtaacg tgcggtttaa acattttgca
     7141 tccttaatct gtcacttctc gccattcgcc attggcaaga ttatccaaag agtaatctcc
     7201 catcgcatag cgaatcagtc gcagcgtggg gaaaccaaca tgggcggtca tgcggcgcac
     7261 ctggcgatta cgtccttcat ataaggtgat cttcagccag ctggtgggaa tacttttgcg
     7321 ttcacgaatt ggtggattcc gcggccataa ccacgcgggt tcgtcaacca gctccgcgcc
     7381 tgcgggcagg gtagggccat catttaaggt tacgccattg cgcaaggctt caagtgcgtc
     7441 ttgtgtggga ataccttcca cctgcacata atagattttt ccggtgcgtt tacccggctg
     7501 ggttaaacgc gcctgcaacg cgccgttatt ggtcagcacc agcaaccctt cgctatcgcg
     7561 gtcaaggcga cctgctgcat aaacgccctg aaccgggatg aattctttta atgttttgcg
     7621 tccggcttca tcggtgaact gcggaagaac atcgtagggt ttattgaaga ggatcacacg
     7681 cgtgggctgg ttttcatgtt tacgcctggt agaacgttgc gagctgaatc gcttaacctg
     7741 gtgatttcta aaagaagttt tttgcatggt attttcagag attatgaatt gccgcattat
     7801 agcctaataa cgcgcatctt tcatgacggc aaacaatagg gtagtattga caagtcaatt
     7861 acaaatcatt aacaaaaaat tgctctaaag catccgtatc gcaggacgca aacgcatatg
     7921 caacgtggtg gcagacgagc aaaccagtag cgctcgaagg agaggtgaat ggaaagtaaa
     7981 gtagttgttc cggcacaagg caagaagatc accctgcaaa acggcaaact caacgttcct
     8041 gaaaatccga ttatccctta cattgaaggt gatggaatcg gtgtagatgt aaccccagcc
     8101 atgctgaaag tggtcgacgc tgcagtcgag aaagcctata aaggcgagcg taaaatctcc
     8161 tggatggaaa tttacaccgg tgaaaaatcc acacaggttt atggtcagga tgtctggctg
     8221 cctgctgaaa cccttgatct gattcgtgaa tatcgagttg ccattaaagg cccgctgacc
     8281 actccggttg gtggcggtat tcgttctctc aacgttgccc tgcgtcagga actggatctc
     8341 tacatctgcc tgcgtccggt acgttactat cagggcactc caagcccggt taaacaccct
     8401 gaactgaccg atatggttat cttccgtgaa aactcggaag acatttatgc gggtatcgaa
     8461 tggaaagctg actctgccga cgccgagaaa gtgattaaat tcctgcgtga agagatgggc
     8521 gtgaagaaaa ttcgcttccc ggaacattgc ggtatcggta ttaagccgtg ttctgaagaa
     8581 ggcaccaaac gtctggttcg tgcagcgatc gaatacgcaa ttgctaacga tcgtgactct
     8641 gtgaccctgg tgcacaaagg caacatcatg aagttcaccg aaggcgcgtt taaagactgg
     8701 ggctaccagc tggcgcgtga agaatttggc ggtgaactga tcgacggcgg cccgtggctg
     8761 aaagttaaaa acccgaatac cggcaaagag atcgtcataa aagacgtgat tgctgatgca
     8821 ttcctgcaac agatcctgct gcgtccggct gaatatgatg ttatcgcctg tatgaacctg
     8881 aacggtgact acatttctga cgctctggca gcgcaggttg gcggtatcgg tatcgcccct
     8941 ggagcaaaca tcggtgacga atgcgccctg tttgaagcca cccacggtac tgcgccgaaa
     9001 tacgccggtc aggacaaagt aaaccctggc tctattattc tctccgctga gatgatgtta
     9061 cgccatatgg gctggactga agccgcagac ctgattgtta aaggtatgga aggcgcgatc
     9121 aacgccaaga ccgtaaccta tgacttcgag cgtctgatgg aaggcgctaa actgctgaaa
     9181 tgttcagagt ttggtgatgc gatcatcaag aatatgtaat cactacatgt gttaaatatt
     9241 taaacgggcg tataacacgc ccgttgtttt atttatgtgg atattattaa tagcatatcg
     9301 agcatattta tataaaacct attacttgag cccatatggg catattttta taatacaact
     9361 cttatgcaaa catttatttg ttatttcgct ttctcctgga ggacactctt gactgctttt
     9421 gagtaaactc cataaattct tgttgaatgg tgcaatgtta taaatagtaa taggatattc
     9481 tttttcctta aggataatac caagcttaac cggtgtaaat atccttccag gaggaagaaa
     9541 tatagtagat tgataccaga tgatcatttt catatcaccc catatggctg aaaaagatat
     9601 gccgcatgta ggttgaatta ccgtgtcaat tactacccac ttcatttgtt atgtcttatc
     9661 ccacggtatt taatatagtt catttggatg ttcatttctt tattttgcat atgagtatat
     9721 taccccttca aaaaataaat taattaaaac gattgcttat ataaaacaaa atttaaatca
     9781 aggaatctca atggatgtta aacaaaatga gatttagtga aaacaataaa ttattcactt
     9841 cgttttagat ttgtttagct ataatgttat acattcaaat gactgaacat cctgtaatta
     9901 aaacatagcc tttatgctac ttggtgccaa tttgctaaac attatggttg cctttttata
     9961 taacgataat aatgaatata aacatgacat gagaataagg tttcaatttt tgagttatat
    10021 aggaaaggtt taacctgttc ctggctaaaa tacatataac tggatgatga ctaaaccaaa
    10081 acacatatgc gttaagtatt gaaacgggcg tatggcacac ccgttgtttt tataaatata
    10141 ttaaccgtta taaaataacg tataaaaagt caagtgatca catttcaaat atcaatttga
    10201 tagtatgggc ttggtgatta tttatgggta gcaataaaag gacagtattt atcatccata
    10261 gggatagtct ctgtactttt attcccatta tgctaatgcc ttactgaatt atgaagcatt
    10321 tcttaagcat ccaactttag ctagattaat ggtttattat tttctacatc ttcaatatat
    10381 aaaagcgtat tatcaatggc gtagtaactg cgtttgttat gattaacatc agtaacccaa
    10441 cggaaaacgc ccgcgcctgc tagtgttgaa cagtattccc gaaatgtaga ttttccgcaa
    10501 atatgaagca atgcggcctc ttttatttta gcaggattct tggtcgtact aacttttaac
    10561 tggttcctgg ttcctcttaa taacaaaacc gtgtcatcgt gagtaataat tctgatgtta
    10621 tccgtagcca gataataaat gtaatgtgca atacggtgat gttttaattc tgaataaaac
    10681 caggagaagt tttgctcttt tctcacttgc tcaaacatct tttgaaaaac aacgacctga
    10741 tccatcagga taataacctc ttgttagttg tgagactgcg tagtgtgcac gatcggtttt
    10801 accacttcaa tctggtctgt cctttggctg tgatatgtac agagtgtgat agagggatta
    10861 cctgaattct cccggtgagc attttgcaac ggaccagctc cggtacaaat gctgttgtgg
    10921 gttcagatta taacattctg tctaaggggc gggataaagg tgaaattagg gggcatgaaa
    10981 gatgacttta taaccttgct caccccaatg ttgtaaaagt tcgttttgcc ttctcgttgg
    11041 tgccatgcct gtccagacaa tcaatgtttg cgtcgggaac agttccgggc gcggcgattc
    11101 aatgggatca gcaagaacag aaatgtgcca tcctgacaat gataaacgcc atgcttccag
    11161 ccacaatcgt gctctctctt caacattcca cgccatcaga atggcttctt taccgggctt
    11221 acggcgcgtt tcgaaaagcg aggttgctgc gtattcaatt aatgcgccgt caaacatact
    11281 gctcataatg cgggaggtgt tgtgatcaag cacgagacgc tggcgaacag gaaggtaaac
    11341 atgattaatc aattgatcta ctgggtactc tcgacccagt gaaataattc tcgcgcgtag
    11401 tttggcagga ttagccatac gaagaattga catcatctct tcttgcaggc ggctccagtc
    11461 atcttccgta tcctggctgg tggtttccag taatgcttta actttgccta cagggacgcc
    11521 attactgatc caacgcttga tctcttcgat gcgttgtatg tcttcttcat caaagagtcg
    11581 gtgtccgcct tcactgcgct gtggttttaa caaaccgtag tggcgttgcc aggcccggag
    11641 agtgacagga ttaatcccgc aacgttcagc aacatcacca atgctgtaat aagccacaat
    11701 tcctccttgc ggtcacaaat ctccgtcgcc ggtacacgac ccaataatac tttgtacaat
    11761 atacgctaaa attgtacaaa gtataaataa gatttcagct aaattggatg aaacattatt
    11821 tttaatgtag attaaattta aacataacgt attcattttc acgatgattt actgaaatca
    11881 tgtgaaagaa tgtgctgaaa attatttttt ctctggccac gctatggaag ggataccatt
    11941 caatttagct ttagcaaaca gatctccctg aaacatctca attcctgcgg attcaagcca
    12001 catccactct tctggtgttg ccacgcccat agcactgact tgaatttcaa gtgatgtaca
    12061 gcattttatg atcgcttgaa taattgcctg ccgtggcccg cttttatgaa cattggtaat
    12121 caattcctga ctgattttaa ttctgtcagg ctggaagcgt gacaggagta acaaaccggc
    12181 aaaacctgcg ccaaaatgat caattgctac actgataccg gcagccttaa gcgatttaat
    12241 cgcttctgca aactcatcaa accgagatat aacttcactt tcagtaaatt caacgatgat
    12301 ttgttcaggc accagagcat tggcctttat ttcatcaagt aaaaaagaga ctgcgtcagg
    12361 ttcgttaacc agggtcatag gtaatagatt gattgaaatc attttatcac cgagctcaag
    12421 tgcgtgtgtc atcgcgaatg caagcgcctt acttttgaga tccgctgtgt agatttcccc
    12481 gtctttacgc tgcccaaccg ctatggctga tgggctatct tcattttttt gcacaatggc
    12541 ttcaaaagcg attatccgcc gcgataaggg atcgacaata ggatgaaagg caaagtggtc
    12601 gtttatagtt ggtgaaaggg tacaggaatc aagttcttta tcagatccgt cagcgataaa
    12661 aagccaggag tcttcggcag ggatctcgaa ataggttgat tgttcggttg caaggacaaa
    12721 agtacgaaaa aattgtagcg ctctgtcatc ataagttagc tgaaattttg atgtgccttt
    12781 gtcgaatacg gcctgtaaaa cgtcatctcg ctcgtgcagg cgcaaatcaa ataattccat
    12841 tcccgctttg ccaaaacggc gagcaggcgc gtaatcgcac agcagctcaa caatattata
    12901 gtgccgtgga tcttggcata tagcccgata tatcatttta acctgttctt ccggaccttc
    12961 cagaagctgg aaaaaatgag aaccattaaa cagtaagatc cctgttacgt cagactgcat
    13021 gttcctgcga tttgctatcg aaaccatttc ttcgattttt ttgacaggtt cgtcgtcacg
    13081 tatatggcta cgataaataa gggtggtaag cattaacaat ccagggtaat gggtgaggcg
    13141 agagtaagac ggtaacagac atatcttctt gtgtctttct tttaatacca aaacataacc
    13201 gtttctttac attgataaaa aatggaaaaa gttgaacacg agttggcgaa aaatcttgta
    13261 tagattgtca gttaaatgat gcaatatgtt ttatcataac acattgtttt atatacatta
    13321 gtactaattg caaaaaatta atttatcatt ctgtacacat atttcgtaca agtttgctat
    13381 tgttacctca cttcacattg attaacattt ttaacagagg cgtagcatgc atcaaaattc
    13441 agtgacttca gattctgcgg gagcaatcac tcgttacttt gcaaaagcta acttgcctac
    13501 tcagcaggaa actctcgggg aaattgtgac tgagattttg aaagatggac gtaatctgag
    13561 tcgaaagtcg cattgtgcca aacttctttg ccgactggaa caagcgacag gggaagagga
    13621 acagaaacat tataatgcac taattgggtt gctttttgaa taatgcaaca gcctgattat
    13681 ttaacaggtt agctatgaag tcgttatgaa gacatctgat aatgaacgta taaaatatga
    13741 aattactggc caggcggtgc tccagatact gcgcatgaag ataaattttt cattgcagac
    13801 tcttattaag caattactcg taatgaaatc ggctgaggaa gatgctttcc gacgcgattt
    13861 aattgacagc ataattcgtg attttagcaa tagtgattca ggagggccaa accgaagaac
    13921 agcgacagct gacaataaaa gtatgttcaa tggtaagaaa ataaacagaa tacattaaaa
    13981 tttcataagt aagatgagag gttaccatgc ttgaagatac tacaattcat aatgcaataa
    14041 ctgataaagc gttagcaagt tactttcgca gttcgggtaa tttgttagaa gaagagtcag
    14101 cagtgttagg gcaggctgtc accaatttaa tgctttcagg cgataatgtt aataataaaa
    14161 atattatctt aagtctgata cactccctgg aaacaacaag tgatattctc aaagctgatg
    14221 tgattagaaa aacactggaa atcgtgttgc gatacacagc tgatgatatg taacttctaa
    14281 ttattaagta taagtttata gaaaactcat tcatcttatt tttgtctgtc gctttagact
    14341 atacacagca taattttatt gggttaatat ttctacgagg ctgacatgaa taattcaatc
    14401 ccagagagat ttatttttca atgtgctttg tttaaaaatc tcgagaggga ggtgttcatg
    14461 acgcatgggt atgttgatag tcatattatt gatcaggctt tacgcttgcg tttaaaagat
    14521 gaaaccagtg tgattctttc tgatctctat ctgcaaatat tgcagtacat tgaaatgcat
    14581 aagactacac taacggatat cattattaat gacagggaat ccgtgctctc ttagaacttg
    14641 agtaaggttt tagaataaac atcagctgta tcaccatgct gatgcaaagt gagcatttca
    14701 ggcgttatgc tttcttatta tgtccgcaat atcaggtgtc aagaatggag agttctcgct
    14761 ctccattctt gacgcctgat atcccgccta acttattttg tactgcctac attatgctca
    14821 ataattaatg ggttagatca agatggagaa aattttatct tcggcgtgga tatttcttat
    14881 gctctctacc atgcatctct gtagatgcca gcgcgagcga atgaatgg
//
LOCUS       DADMGC010000100        14924 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.100, whole genome shotgun sequence.
ACCESSION   DADMGC010000100 DADMGC010000000
VERSION     DADMGC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.100"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            596..793
                     /locus_tag="JNZ73_002984"
     CDS             596..793
                     /locus_tag="JNZ73_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733199.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(743..1936)
                     /locus_tag="JNZ73_002985"
     CDS             complement(743..1936)
                     /locus_tag="JNZ73_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8733200.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2072..3796
                     /gene="iucA"
                     /locus_tag="JNZ73_002986"
     CDS             2072..3796
                     /gene="iucA"
                     /locus_tag="JNZ73_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HAY8733201.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3797..4744
                     /gene="iucB"
                     /locus_tag="JNZ73_002987"
     CDS             3797..4744
                     /gene="iucB"
                     /locus_tag="JNZ73_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HAY8733202.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4744..6486
                     /gene="iucC"
                     /locus_tag="JNZ73_002988"
     CDS             4744..6486
                     /gene="iucC"
                     /locus_tag="JNZ73_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HAY8733203.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAEVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6483..7799
                     /gene="iucD"
                     /locus_tag="JNZ73_002989"
     CDS             6483..7799
                     /gene="iucD"
                     /locus_tag="JNZ73_002989"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HAY8733204.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSPASLTHYTANIQE"
     gene            7805..10000
                     /gene="iutA"
                     /locus_tag="JNZ73_002990"
     CDS             7805..10000
                     /gene="iutA"
                     /locus_tag="JNZ73_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HAY8733205.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            10944..14831
                     /gene="sat"
                     /locus_tag="JNZ73_002991"
     CDS             10944..14831
                     /gene="sat"
                     /locus_tag="JNZ73_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HAY8733206.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPEETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSTTSHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
BASE COUNT         4020 a         3554 c         3706 g         3644 t
ORIGIN      
        1 gagtgaaaca aaggcagctt atcgttgttt ttcaaacgat aaagtctctg ctaataaaat
       61 catggctcct cacaaagaaa atatcactga aagagctcag cagtttaaaa gagttttagt
      121 tttacaggat acaacagaac ttaattattc tggtcagaaa gaaaaacaag gagttggtcc
      181 caaaaaacat aaagatgaaa gaaacttatt tcttcatcct caactcgtca tcagtgagtc
      241 aggtgtttgc ctcggtgttt atgatgacta tcagtggttc agggatgaat taaaaaccag
      301 taaaaatacc cgacaagaaa tctgcaatga tttacttcat aaaaaacatg tcagtgaaaa
      361 agaaacctgg cgttgggttg aaggatataa taaagcaaca gaactggctc gccaatgccc
      421 tgatacccat gtattgtcaa tttctgatcg tgaaggcgat ttttatgacc tgtttgaacg
      481 cgcagcgcag actcccggaa taaaagctga ctggcttgtc agaatgaaat ttaataacag
      541 agcaacgctg aatattaatg gtaaacggga tcataggttg ttgcacgaaa gaatcatgga
      601 gattacaccg cagcaactgg ttgaattcac tatacctgat gggcgggggc agcaagccag
      661 aacactgcct gacatgcttt catcagacgc aggtcctctc tcatatcctc tgatttactc
      721 cagtaaggtg atgatcatat ctttatttga tagtacggta atgccagata aatacagaca
      781 caatggtaaa taatatagtt gcaaataatg ctgccataat cagcgcgatt gcgacacctg
      841 ttttcgatac cagcggcacc agcaaagcgc acagtccata gccaagtgtg tgactggtag
      901 cgagccagcc cgcgcctgcg ccatcagcca gcttatcatt cagtagaagt tgataagcgg
      961 gtgttgccag agctgctcca aatgacagca ctgcacagcc cgggtaaaac aaccatagct
     1021 gttcggagag catgatagcc agaccaccac tcatcaacac tccggcactc aggagcaggg
     1081 ccaccggagt caggcgctgc ggacggagta ccccgaactg cgcgataagc gcagctactg
     1141 cggacagacc caacagccag gccacctgtt ggctaatggc ggtggtatcc gtagcgaact
     1201 ggcgagtaag ggcaggcgaa agtccaagct gcatcatgct caccgccgca gccagtaata
     1261 ttgcgcaaag cagataaggc agacaatccc gcttcagact gacgctcttg cactccggtg
     1321 tgggctgtgg tggtgtgccg ggcaaccgca gcagcatcag cagcgccagc actggggctg
     1381 ccatcagcag ccccagtggc gccagaggat gaatggccag cattgctgcc gcgcacagcg
     1441 gcccgaatag cctgccgcaa ctcaggccgg agctgatggt tgccagagcg gccatgcgat
     1501 tcccttctcc agctctctgc aatgcccaga cctgacaggc tggcaccatt gccgacacgg
     1561 tcagcccgta ggcgatccgg gcgatgatta atattcccag tcccaccgct gttgttatgg
     1621 cgctggtagc catcagcacg cttcccagcc ctagcagggt aaagcttatg ccatatcctc
     1681 ccagagccca caacaccaca ggtttactgc cagtacggga aatttgcttt ccccaccacg
     1741 gggacgatgg cagaaacagc attgagccaa gcatcagcag ggcagcccat acagacagac
     1801 tcagatttgt ctgtatgacc agaacaggga gcacaaccag cagaccgttc tgtccgatac
     1861 cgagaagccc ggcactgaac gcaagtggcc agcaggacag tggtttttgg ggcgtatctt
     1921 cgatccctgg tgacattata atgtttcaac tccatgtatt aattgtgttt atttgtaaaa
     1981 ttaatttatc tgacaataac atttattgtt gataatgaga atcattattg acataattgt
     2041 tattatttta ctgtgtggga gctgtttgac tatgaccctg ccctctgaaa aaccagccac
     2101 agatgtggct gcgcagtgct tcctgaatgc actgattcgt gaaaccacag actggaaact
     2161 gacagaatac ccgccagacg aattgctcat cccgctggat gagcagaaat cgctccattt
     2221 cagagtggct tatttctccc caacccagca tcaccgcttt gcatttcctg cacgtctggt
     2281 cacggcatca ggcagttatc ctgtcgactt taccactctc tcccggctta ttattgataa
     2341 gctacgccat caactgtttc tgcccgttcc cctctgcgaa actttccacc agcgcgtgct
     2401 ggaaagccat gtccatacgc aacaggcaat tgatgcccgt catgactggg ccgctctgcg
     2461 tgaaaaagcg ttgaattttg gcgaggctga gcaggcgctg ctgacaggac acgctttcca
     2521 ccctgcgcct aagtctcatg aaccgtttaa ccggcgggag gctgaacgct acctgcctga
     2581 tatggcaccc cacttcccac tgcggtggtt ttcggtggat aaaacgcaaa tcgccggtga
     2641 aagtttgcat ctcaaccttc aacagcggct gacgcgattt gccgcagaga atgcgcctca
     2701 gttactcaac gaattaagtg acaaccaatg gctgttcccg ttgcacccgt ggcaggggga
     2761 atatcttttg cagcaggggt ggtgccaggc acttgttgct aaagggctga ttaaagactt
     2821 aggtgaggcc ggcacgtcgt ggctgccgac cacctcttcc cgttccctct actgtgccac
     2881 cagccgcgat atgatcaagt tctccctgag cgtacggctg accaactcca tccgtactct
     2941 gtccgtgaaa gaagtgaagc gtgggatgcg cctggcacgc ctggctcaaa ccgacggctg
     3001 gcagatgcta caggtccgct tcccgacttt ccgggtaatg caggaggatg gctgggccgg
     3061 gctgctcgat cttaacggca acatcatgca ggaaagtctg tttgccctgc gtgaaaatct
     3121 gctggtggat cagccgaaaa gccagactaa cgtactggtc tccctgactc aggccgcacc
     3181 tgatggcggt gattcgctgc tggtttcggc ggtaaaacgc ctgagcgatc gcctcggtat
     3241 cactgtgcaa caggccgccc atgcatgggt cgatgcatac tgtcagcaag tgctaaagcc
     3301 gctgtttacg gctgaagcgg attacggcct ggtgctgctg gcgcatcagc aaaatattct
     3361 tgtccagatg cttggggatc tgccggtcgg atttatttac cgtgactgtc agggcagcgc
     3421 ttttatgcct cacgcgacag actggctcga ttctattggc gaggcgcagg cggaaaatat
     3481 tttcacccat gagcagttgc tgcgctattt cccttattac ctgctggtta actccacttt
     3541 tgctgtgacc gctgcgctgg gggctgccgg gctggacagc gaatcgaatc tgatggctcg
     3601 tgtacgagca tcgctggctg aagtgcgtga tcaggtgact cataaaacct gtctcaacta
     3661 cgtgctggaa agtccgtact ggaacgtaaa aggtaacttt ttctgttatc tgaacgatca
     3721 taacgagaac accattgttg acccttcggt gatctatttc gatttcgcta acccgctgca
     3781 ggctcaggag gtctgaatgt ctgaggcaaa cattattcac agcagatatg gactgcgctg
     3841 tgaaaaactc gacaagcccc tgaatcttgg ctggggactg gacaatagcg cggtgttgca
     3901 ctgtcccggg gagctgccga cagggtggct gtgcgatgcg ctggatcaga tatttatcgc
     3961 cgcaccacaa ctgtcagcag tggctctccc ctgggccgaa tggcgtgagg agccacaggc
     4021 gctgacgctt tttgggcagg taaaaagcga cattatccat cgtactgcct tctggcagtt
     4081 accgttatgg ttgagttcac cggcaaaccg ggcctccggt gaaatggttt ttgatgcaga
     4141 gcgtgagatt tatttcccgc agcgcccacc ccgtccacag ggtgaggttt accgccgtta
     4201 cgatccacgt attcgcagga tgctgagttt ccgcattgcc gatcccgttt ctgatgcaga
     4261 acgtttcacc cgctggatga atgatccgcg cgttgagtat ttctgggagc aaagtggctc
     4321 actggaggta cagaccgcct atctggagcg ccagctcacc ggtaaacatg cattcccgct
     4381 gattgggtgc ttcgacgatc ggccattcag ctatttcgaa atctactggg cggcggaaga
     4441 ccgcattggt cgtcactatt cgtggcagcc ctttgaccgt ggcctgcatc tgctggttgg
     4501 tgaacagcaa tggcgcgggg cccactatgt gcaaagctgg ctgcgcgggc tgacacatta
     4561 cctgctgctg gatgagcccc gtacgcagcg cacagtactg gagccacgca ccgataacca
     4621 gcgcctgttc cgccatcttg agcctgcggg ataccggaca attaaagagt tcgacttccc
     4681 acataagcgc tcgcgcatgg tgatggcgga tcgtcatcac ttcttcacgg aggtcggtct
     4741 gtaatgaatc acaaggactg ggattttgtt aaccgtcggc tggtggcaaa aatgttgtct
     4801 gagatggagt atgagcaggt tttccacgcg gaatctcagg gcgatgacca ctactgcatt
     4861 aacctgccgg gagcacaatg gcgcttcatc gctgaacgtg gtatctgggg ctggctgtgg
     4921 attgatgccc aaacgttgcg ctgcacggac gaaccagtac tggcgcagac gctgctgatg
     4981 cagctaaagc cggtgctgtc aatgagtgat gccactgttg cagagcatat gcaggatttg
     5041 tatgccacgc tactgggcga cctgcaactg ctgaaggccc gtcgcggact gagtgccagt
     5101 gacctgatta atcttgatgc cgatcgtctg caatgcctgc tgagcggtca tcctaaattc
     5161 gtttttaata aaggtcgccg tggctggggc aaagaggcgc tggaacgata tgcgccagag
     5221 tataccaaca ccttcagact gcactggctg gcggtaaaac gtgaacatat gatctggcgc
     5281 tgcgataacg atctggatat tcagcagttg ttgactgccg caatggatcc gcaggagttt
     5341 acacgcttca gtcaggtctg gcaggaaaac gggctggatc acaactggct gccgctgccg
     5401 gtacatccgt ggcagtggca gcaaaaaatc gccacagact tcatcgctga ttttgccgaa
     5461 ggcaggatgg tgtctctcgg cgagtttggc gaccagtggc tcgcccagca gtcgctgcgt
     5521 accctgacca acgccagccg ccggggaggg ctggatatca agctgccgct gaccatctac
     5581 aatacctcat gctaccgggg gatccctggc agatatatcg ctgccggacc actggcttca
     5641 cgctggctgc aacaggtttt tgcgaccgac gccaccctgg tgcaaagcgg cgcagtgatc
     5701 cttggtgaac cggctgcagg ctatgtgtcc catgaaggct atgccgcgct tgcccgggct
     5761 ccctatcgct accaggaaat gcttggtgtt atctggcggg agaatccgtg ccgctggctg
     5821 aaaccggatg aaagtcctgt tctgatggca acactgatgg agtgcgacga aaacaatcag
     5881 ccgctggcag gcgcatatat cgaccgctcc ggactggacg ctgaaacctg gcttacgcaa
     5941 ttgttccggg tggttgtggt tccgctgtat cacctgcttt gccgctacgg tgtcgcgctt
     6001 attgcacacg gacaaaatat aacgctcgcc atgaaagagg gggtaccaca gcgtgttctg
     6061 ctgaaagact tccagggcga tatgcggctg gtgaaagaag cgtttcccga aatggactct
     6121 ttgcctcagg aggtgcgtga tgtcacatcc cgtctgagtg cggactactt aatccatgat
     6181 ttgcagacgg gtcacttcgt gacagtactg cgttttattt cgccactgat ggttcgtctt
     6241 ggcgtacctg aaaggcgatt ttatcaactg ctggcagaag tgttgagtga ttacatgaac
     6301 aaacatccac aaatggcaga gcgttttgcg cttttctcac tcttcaggcc acaaatcatt
     6361 cgcgtggtgc tgaacccggt aaaactgacc tggccggacc tggatggcgg cagtcgcatg
     6421 ctgccgaatt accttgagaa tctgcaaaat ccgctgtggc tggtaactca ggaatatgaa
     6481 tcatgaaaaa aagtgtcgat tttattggtg tcgggacagg gccatttaat ctcagcattg
     6541 ctgcattgtc acatcagatc gaagaactga actgtctctt ctttgacgaa catcctcatt
     6601 tttcctggca tccgggtatg ctggtaccgg attgtcatat gcagaccgtc tttctgaaag
     6661 atctggtcag tgcagttgca cctacaaatc cctacagctt tgttaactat ctggtgaagc
     6721 acaaaaagtt ctatcgcttc cttacaagca ggctacgtac agtatcccgt gaagagtttt
     6781 ctgactatct ccgctgggct gctgaagata tgaataacct gtatttcagt cataccgttg
     6841 aaaatattga tttcgacaaa aaaagtcgat tgtttctggt tcagaccagt cggggagaat
     6901 attttgccca caatatctgc cttggtacag gaaaacaacc ttatttacca ccctgtgtga
     6961 agcatgtgac acaatcctgt ttccatgcca gtgaaatgaa tcttcgtcgg ccggacctga
     7021 gtggaaaacg gataaccgtg gttggtggag gacagagtgg tgcagacctg ttccttaatg
     7081 cattacgcgg ggaatgggga gaagcggcgg aaataaactg ggtctcacgg cgtaataatt
     7141 ttaacgcact ggatgaggct gcttttgctg atgagtattt tacacctgaa tatatttcag
     7201 gcttctccgg actgaaggaa gatattcgcc atcagttact ggatgagcag aaaatgacat
     7261 cggatggcat cactgccgat tctttactga ccatttatcg tgagttgtac caccgttttg
     7321 aagttctgag aaaaccaaga aatatccgtc tgctacccag tcgctcggta acaactctgg
     7381 aaagtagtgg tccgggctgg aggttattga tggaacatca tctggatcag ggcagagaga
     7441 gcctggaaag tgatgtggtg attttcgcca caggttaccg ttctgcattg ccacaaatac
     7501 ttccctcact gatgcccttg atcaccatgc acgataagaa cacctttaaa gtgcgtgatg
     7561 acttcactct ggaatggagt ggcccgaaag aaaataacat ctttgcggtc aacgccagca
     7621 tgcaaactca tggcatcgcc gaaccccagc tcagcctgat ggcctggcga tctgcacgta
     7681 ttcttaatcg cgtactgggg cgtgatttat tcgatctcag tatgccgccc gcactgattc
     7741 agtggcgcag cggcagccct gcttctttaa ctcactatac agcaaatatt caggaataac
     7801 gatgatgcgc aaaaagtata tgccccgggc tcttggtccg ctgcttcttg tcgtgctgtc
     7861 accagctgtc gcccagcaaa acgatgataa tgagatcata gtgtctgcca gccgcagcaa
     7921 tcgaactgta gcggagatgg cgcaaaccac ctgggttatc gaaaatgccg aactggagca
     7981 gcagattcag ggcggtaaag agctgaaaga cgcactggct cagttaatcc ccggccttga
     8041 tgtcagcagc caaagtcgaa ccaactacgg tatgaacatg cgtggccgcc cgctggttgt
     8101 cctgattgac ggtgtgcgcc tcaactcttc acgttccgac agccgacaac tggactctgt
     8161 cgatcctttt aatatcgacc atattgaagt gatctccggc gcgacggccc tgtacggtgg
     8221 cgggagtacc ggagggttga tcaacatcgt gaccaaaaaa ggtcagccgg aaaccatgat
     8281 ggagtttgag gctggcacaa aaagtggctt taacagcagt aaagatcacg atgagcgcat
     8341 tgccggtgct gtctccggcg gaaatgacca tatctccgga cgtctttccg tggcatatca
     8401 gaaatttggc ggctggtttg acggtaacgg cgatgccacc ctgcttgata acacccagac
     8461 cggcctgcag cactccaatc ggctggacat catgggaacc ggtacgctga acatcgatga
     8521 atcccggcag cttcaactga taacgcagta ctataaaagt cagggagacg acaattacgg
     8581 gcttaatctc gggaaaggct tttccgccat cagcgggagc agcacaccat acgtcagtaa
     8641 ggggctgaat tctgaccgca ttcccggcac tgagcggcat ttgatcagcc tgcagtactc
     8701 tgacagtgat ttcctgggac aggaactggt cggtcaggtt tactaccgcg atgagtcgtt
     8761 gcggtactac ccgttcccga cggtaaatgc gaataaacag gcgacggctt tctcctcgtc
     8821 acagcaggat accgaccagt acggcatgaa actgactctg aacagccaac tgatggacgg
     8881 ctggcaaatc acctgggggc tggatgctga gcatgagcgc tttacctcca accagatgtt
     8941 cttcgatctg gctcaggcaa gtgcttccgg agggctgaac aaccataaga tttacaccac
     9001 cgggcgctat ccgtcatatg acatcaccaa tctggcggcc ttcctgcaat ccagctatga
     9061 cattaatgat atttttaccg ttagcggtgg cgtacgctat cagtatactg agaacagggt
     9121 agatgatttc atcgactaca cgcagcaaca gaagattgct gccgggaagg cgatatctgc
     9181 cgacgccatt cctggtggtt cggtagatta cgataacttt ctgttcaatg ctggtctgct
     9241 gatgcacatc accgaacgtc agcaggcatg gttcaatttt tcccaggggg tggcattgcc
     9301 ggatccgggg aaatattatg gtcgcggcat ctatggtgca gcagtgaacg gccatcttcc
     9361 cctgacaaag agcgtgaacg tcagcgacag taagctggaa ggcgtgaaag tcgattctta
     9421 tgaactgggc tggcgcttta tcggtgacaa cctgcggact caaatcgcgg catattactc
     9481 gctttccaat aagagcgtgg aaaggaataa agatctgacc atcagtgtga aggacgacag
     9541 gcgccgtatt tacggcgtgg aaggtgcggt ggactacctg atcccggata ctgactggag
     9601 taccggtgtg aacttcaatg tgctgaaaac cgagtcgaaa gtgaacggtc aatggcaaaa
     9661 atatgacgtg aaggaatcaa gtccatcgaa agcgacagct tacattaact gggcgccgga
     9721 accgtggagt ctgcgtgtac agagcaccac ttctttcgac gtaagcgatg cagagggtaa
     9781 cgatattaat ggttacacta ccgtcgattt tatcagtagt tggcagcttc cggtgggaac
     9841 actcagcttc agcgttgaga acctcttcga ccgtgactat accactgtct ggggacagcg
     9901 tgcacctctg tactacagcc cgggttacgg ccctgcttca ctgtacgact acaaaggccg
     9961 gggccgaacc tttggtctga actactcagt gctgttctga cggggatgct tttgcaacaa
    10021 gggtatgctg taaacaacat ggtccttggt ggccatgttg tttacagcca gtataatata
    10081 cagccttaac aagatattcc gtctccggaa tgggctgttc accactgaat aacattatgt
    10141 ctgttttatg gatctttgac tgaaatttat cgcatatcct catttcattg tccgtctgaa
    10201 aactaacctt acaagtaagc gtcgtctcag caccgtctgg cagatcctga aattcctgag
    10261 aggatgatct gcgcccaata tcctgagccg gaactttgct gcaatgaagg ccaacgaaaa
    10321 atgggggagt atgttataga gtacttgcta cagggtaaaa agaggtacct gtcgctggtt
    10381 ctccgttcag tttaaccggg agattttctc ctgcgcctgt cataaagtcc ggtaatggag
    10441 attgttaata cgatggtgca cgatgcatcg tcagtgcccg gcccggagga tgccccgtag
    10501 ctacatacgg atcagggttg gcaatatcga atggcaggct ataggcaaag ttaaagcccc
    10561 atggagtagc acaaaatatg ccgcacaaag gaaacggtct gaataacgca gtgatggaga
    10621 acttcttcgg cacactgcaa aatcgcatcg tggtcgatcg ctaattcgtc gtgaatcact
    10681 gaacgatgtt ccatgcgaac agatattcga tcatatcgaa atattaaaca ttacccctcc
    10741 ccacattggg caaaaaaaaa caaaaaacga tcaattaacg atcaattagt attgattcac
    10801 ataatctctc atcattaatg agaacattgt cacatattta caatatagaa ctgttacttt
    10861 tagcaagctg ttagtaacca accgttacta ataattttaa ttcttaatac tttgttcatt
    10921 caataaatga gttgagagaa tatatgaata aaatatactc ccttaaatat agtgctgcca
    10981 ctggcggact cattgctgtt tctgaattag cgaaaagagt ttctggtaaa acaaaccgaa
    11041 aacttgtagc aacaatgttg tctctggctg ttgcaggtac agtaaatgca gcaaatattg
    11101 atatatcaaa tgtatgggcg agagactatc ttgatcttgc acaaaataaa ggtattttcc
    11161 agcccggagc aacagacgta acaatcactt taaaaaacgg agataaattc tctttccata
    11221 atctctcaat tccggatttt tctggtgcag cagcgagtgg cgcagctacc gcaataggag
    11281 gttcttatag tgttactgtt gcacataaca aaaagaaccc tcaggccgca gaaactcagg
    11341 tttacgctca gtcttcttac aaggttgttg acagaagaaa ttccaatgat tttgagattc
    11401 agaggttaaa taaatttgtt gtggaaacag taggtgccac cccggaagag accaacccta
    11461 caacatattc tgatgcatta gaacgctacg gtatagtcac ttctgacggt tcaaaaaaaa
    11521 tcataggttt tcgtgctggc tctggaggaa catcatttat taatggtgaa tccaaaatct
    11581 caacaaattc agcatatagc catgacctgt taagtgctag tctatttgag gtcacccaat
    11641 gggactcata cggcatgatg atttataaaa atgataaaac atttcgtaat cttgaaatat
    11701 tcggagacag cggctctgga gcatacttat atgataacaa actagaaaaa tgggtattag
    11761 tcggaacaac ccatggtatt gccagcgtta atggtgacca actgacatgg ataacaaaat
    11821 acaatgataa actggttagt gagttaaaag atacctatag tcataaaata aatctgaatg
    11881 gcaataatgt aaccattaaa aacacagata taacattaca ccaaaacaat gcagatacca
    11941 ctggtactca agaaaaaata actaaagaca aagatattgt gttcacaaat gggggaaatg
    12001 ttctgtttaa ggataatttg gattttggta gcggtggtat tatctttgac gaaggccatg
    12061 aatataacat aaacggtcag ggatttacat ttaaaggagc aggaattgat atcggaaaag
    12121 aaagcattgt aaactggaat gcattgtatt ccagtgatga tgttttacac aaaataggcc
    12181 ccggtactct gaatgttcaa aaaaaacagg gggcaaatat aaagataggt gaaggaaatg
    12241 ttattcttaa tgaagaagga acatttaaca atatatacct tgcaagcgga aatggtaagg
    12301 taatactaaa taaagataat tcccttggca atgatcaata tgcggggata ttttttacta
    12361 aacgtggtgg cacgctagat ttaaatggac acaatcagac ttttactaga attgccgcca
    12421 ctgacgatgg aacaacaata actaactcag atacaacgaa agaagccgtt ctggcaatca
    12481 ataacgaaga ctcctacata tatcatggga acataaatgg caatataaaa ctaacgcaca
    12541 atattaattc tcaggataag aaaactaatg caaaattaat tctggatggt agtgtcaaca
    12601 caaaaaatga tgttgaagtc agtaatgcca gtcttaccat gcaaggccat gcaacagagc
    12661 atgcaatatt cagaagcaca acgagtcatt gctccctggt atttctttgt ggaacggact
    12721 gggtcaccgt tttgaaagaa acagagagtt catataataa aaagttcaat tctgattaca
    12781 aaagtaataa tcagcagacc tcatttgatc agcctgactg gaaaaccggg gtgtttaaat
    12841 ttgatacatt acacctgaac aatgctgact tttcaatatc acgcaatgcc aatgttgaag
    12901 gaaatatatc agcaaataaa tcagctatca caatcggcga taaaaatgct tacattgata
    12961 atcttgcagg gaaaaatatt actaataatg gttttgactt caaacaaact atcagtacta
    13021 atctatccat aggagaaact aaatttacag gtggcatcac tgcacataac agccaaatag
    13081 ccataggtga tcaagctgta gttacactta atggtgcaac ctttctgaat aatactccta
    13141 taagtataga taaaggagca aaagttatag cacaaaattc catgttcaca acaaaaggta
    13201 ttgatatctc cggtgaactg actatgatgg gaatccctga acagaatagt aaaactgtaa
    13261 cgccgggtct ccactacgct gctgatggat tcaggctgag tggtggaaat gcaaatttca
    13321 ttgccagaaa tatggcatct gtcaccggaa atatttatgc tgatgatgca gcaaccatta
    13381 ctctgggaca gcctgaaact gaaacaccga ctatatcgtc tgcttatcag gcatgggcag
    13441 agactctttt gtatggcttt gataccgcct atcgaggcgc aataacagcc cccaaagcta
    13501 cagttagcat gaataatgcg atctggcatc taaatagcca gtcatcaatt aatcgtctag
    13561 aaacaaaaga cagtatggtg cgttttactg gtgacaatgg gaagtttaca acccttacag
    13621 tggacaacct tactatagat gacagtgcat ttgtgctgcg tgcaaatctg gcccaagcag
    13681 atcagcttgt tgtcaataaa tcgttgtctg gtaaaaacaa ccttctgtta gtcgacttca
    13741 ttgagaaaaa tggaaacagc aacggactga atatcgatct ggtcagcgca ccaaaaggaa
    13801 ctgcagtaga tgtctttaaa gctacgactc ggagtattgg cttcagtgat gtaacaccgg
    13861 ttatcgagca aaagaacgat acagacaaag caacatggac tctgatcggc tataaatctg
    13921 tggccaacgc cgatgcggct aaaaaggcaa cattactgat gtcaggcggc tataaagcct
    13981 tccttgctga ggtcaacaac cttaacaaac gtatgggtga tctgcgtgac attaacggtg
    14041 agtccggtgc atgggcccga atcatgagcg gaaccgggtc tgccggcggt ggattcagtg
    14101 acaactacac ccacgttcag gtcggtgcgg ataacaaaca tgaactcgat ggccttgacc
    14161 tcttcaccgg ggtgaccatg acctataccg acagccatgc aggcagtgat gccttcagtg
    14221 gtgaaacgaa gtctgtgggt gccggtctct atgcctctgc catgtttgag tccggagcat
    14281 atatcgacct catcggtaag tacgttcacc atgacaacga gtataccgca actttcgccg
    14341 gccttggcac cagagactac agctcccact cctggtatgc cggtgcggaa gtcggttacc
    14401 gttaccatgt aactgactct gcatggattg agccgcaggc ggaacttgtt tacggtgctg
    14461 tatccgggaa acagttctcc tggaaggacc agggaatgaa cctcaccatg aaggataagg
    14521 actttaatcc gctgattggg cgtaccggtg ttgatgtggg taaatccttc tccggtaagg
    14581 actggaaagt cacagcccgc gccggccttg gctaccagtt tgacctgttt gccaacggtg
    14641 aaaccgtact gcgtgatgcg tccggtgaga aacgtatcaa aggtgaaaaa gacggtcgta
    14701 tgctcatgaa tgttggtctc aatgccgaaa ttcgcgataa tcttcgcttc ggtcttgagt
    14761 ttgagaaatc ggcatttggt aaatacaacg tggataacgc gatcaacgcc aacttccgtt
    14821 actctttctg ataacagccc gggccgcgtt tgcggccctt cttctaccgg agagaatatg
    14881 tattaccctg tgacagacta tatcgctctt gctctcatta ttag
//
LOCUS       DADMGC010000101        14891 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.101, whole genome shotgun sequence.
ACCESSION   DADMGC010000101 DADMGC010000000
VERSION     DADMGC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.101"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            156..1196
                     /locus_tag="JNZ73_002992"
     CDS             156..1196
                     /locus_tag="JNZ73_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HAY8733207.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1229..2422)
                     /gene="lplT"
                     /locus_tag="JNZ73_002993"
     CDS             complement(1229..2422)
                     /gene="lplT"
                     /locus_tag="JNZ73_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HAY8733208.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2415..4574)
                     /gene="aas"
                     /locus_tag="JNZ73_002994"
     CDS             complement(2415..4574)
                     /gene="aas"
                     /locus_tag="JNZ73_002994"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HAY8733209.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            4963..5994
                     /gene="galR"
                     /locus_tag="JNZ73_002995"
     CDS             4963..5994
                     /gene="galR"
                     /locus_tag="JNZ73_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HAY8733210.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDENGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6001..7263)
                     /gene="lysA"
                     /locus_tag="JNZ73_002996"
     CDS             complement(6001..7263)
                     /gene="lysA"
                     /locus_tag="JNZ73_002996"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAY8733211.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7385..8320
                     /gene="lysR"
                     /locus_tag="JNZ73_002997"
     CDS             7385..8320
                     /gene="lysR"
                     /locus_tag="JNZ73_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HAY8733212.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8307..8999)
                     /gene="ygeA"
                     /locus_tag="JNZ73_002998"
     CDS             complement(8307..8999)
                     /gene="ygeA"
                     /locus_tag="JNZ73_002998"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAY8733213.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGKFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAS
                     IHAEDAVAFMLS"
     gene            complement(9128..10516)
                     /locus_tag="JNZ73_002999"
                     /pseudo
     CDS             complement(9128..10516)
                     /locus_tag="JNZ73_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
     gene            complement(10831..11592)
                     /gene="kduD"
                     /locus_tag="JNZ73_003000"
     CDS             complement(10831..11592)
                     /gene="kduD"
                     /locus_tag="JNZ73_003000"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HAY8733214.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11622..12458)
                     /gene="kduI"
                     /locus_tag="JNZ73_003001"
     CDS             complement(11622..12458)
                     /gene="kduI"
                     /locus_tag="JNZ73_003001"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAY8733215.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVAVKD
                     LR"
     gene            complement(12778..13926)
                     /locus_tag="JNZ73_003002"
     CDS             complement(12778..13926)
                     /locus_tag="JNZ73_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAY8733216.1"
                     /translation="MKDVVVVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGG"
     gene            14181..14891
                     /locus_tag="JNZ73_003003"
                     /pseudo
     CDS             14181..14891
                     /locus_tag="JNZ73_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
BASE COUNT         3866 a         4024 c         3511 g         3490 t
ORIGIN      
        1 gcgatgcaaa ttaaccgtga tgatgtttcg caaattattg aacgttaaca aataaggtca
       61 gcatccggct ggccttaaga tttttctctt tcccttttcc cttccctctg ccatttttat
      121 attccttatg tcgtgattat aaaaaggaaa cggctatgca atatcaccgt ataccccaca
      181 gttcgctgga agtcagcacg ctggggcttg gcacgatgac gtttggtgaa cagaacagcg
      241 aagccgacgc ccacgcacaa ctcgactatg ccgtcgctca gggcattaac cttatcgacg
      301 ttgccgaaat gtacccagta cctccgcgcc ccgaaacgca agggttaacc gaaacctacg
      361 tcggcaactg gctggcgaaa catggcagcc gcgaaaagtt aattatcgcc tccaaagtga
      421 gcggaccgtc gcgcaataat gacaagggca tccgcccgga tcaggcgctg gatcggaaga
      481 atatccgcga agcgctgcat gacagcctca agcgcctaca gactgattac ctcgatcttt
      541 atcaggtgca ctggccgcag cgcccgacca actgcttcgg caaactcggt tatagctgga
      601 cggattctgc gcctgcggtt tcgctgctgg atacgctgga cgcactggca gagtaccaac
      661 gcgcgggaaa aattcgttat atcggcgtgt cgaacgaaac tgcatttggc gtaatgcgct
      721 acctgcatct ggcggacaaa cacgatctgc cgcgtattgt caccattcag aacccttaca
      781 gtctgttaaa ccgcagtttt gaagtaggtc tggcagaagt cagccagtat gaaggggtcg
      841 aactgctggc ctattcgtgc ctgggtttcg gcacgctgac cgggaaatat ctcaatggtg
      901 caaaacccgc tggcgcacgt aatacgctct ttagtcggtt cacccgctat agcggtgagc
      961 aaacgcaaaa agccgtcgcg gcgtatgttg atatcgccag acgtcatggc cttgatcctg
     1021 ctcagatggc gctcgcgttt gttcgccgtc aaccgtttgt tgccagcact ctgctgggcg
     1081 caaccacgat ggatcagctg aaaactaaca tcgaaagttt gcatctggag ttaagcgaag
     1141 acgtattagc tgaaattgaa gcggtacatc aggtttatac ttatcctgca ccataaagat
     1201 taacgccggt taaaaccggc gttaaatatt aatgacggcg ctgccagatc cacagcgccg
     1261 ttattgccag cgcaaatagc gcaccgaagc caatgccaat gggcacgacc gggatgccta
     1321 ccattaccgc cagcgagtaa atgcccagca tcaacaacat ggcgctgttt tcgccaaggt
     1381 tttgtactgc aatcgcattc cccgccccga cgcttttttt accccgctcc tgtagcaacg
     1441 cattgagcgg aacgacaaaa aagcccccca gcacgccaat cagcatcaac aaggcatagg
     1501 ctggcagcag ctcgtgttgc agggagaaaa tcagtaccac cacgccaatc aaaatcccgg
     1561 ctggcataca gcgtgacacg gtttccagcg taaccagctt cgctgccgca cctgcgccaa
     1621 ccacgatacc aatcgctacc atcgcgttga gataggtcgg cgtagcgtta tcggtaatgc
     1681 ccagcgccac cggtacccac agcaccaaca ggaaacgcag cgtgacaccc gctccccaga
     1741 ataaactggt gcccaccagc gaaaaacgcg tttcaccatt gcaccatagc gaggtgcagg
     1801 cattcaggaa actgcgggtc atgttgatga gattccagga ctgccccgga cgcgccgccg
     1861 ccagtttggg aatgtagata ttggcaacga ccgcaccacc gtaggccagt gcgcatgcgg
     1921 ccagggcgac caggacatgc cagtcagcca gcacaccacc ggctacggaa ccgagtaaaa
     1981 tcgccgctat ggcagaagct tccattaaac cgttagcttt tactaactta ctacccgtgg
     2041 ttaattcgcc gagaataccg tatttcgccg gtgaataggc tgcagcacca acacccacca
     2101 gcgtatagcc gagaaacgga ttgataccaa agcagatact ggctgcgccc agcagcttca
     2161 ggccgttggc aaacatcatc acccggcctt tggcgaagct atccgccacc tgcccgacaa
     2221 acggcgccaa aagaatgtaa gcacctacaa acaccatttg caggatgggc tggctccact
     2281 ccggatagaa ctgcgctttc agtaacgcca gagtggcaaa cagtagcgcg ttatcgccaa
     2341 acgcagagag aaactgcgcc acgataaccg ctttcatccc cttcgaccac aacgaagtgt
     2401 tagtgtgcac tgactcactc atcgtgttgt tccgcttcgt ctacccagct tttcaacgtg
     2461 acaaagtcag gtttgccgct gccaagtaat ggcatctgtt tcagatagcg aatatcgcgc
     2521 ggtacagcaa gctccggcac gccgtgctcg cgggcatact gttgcaactt atcgcgcgtc
     2581 agttcgttat ctgtggtgaa aagcaccagt gcctcgcctt tgctggcatc gctcttaatc
     2641 gcagtggcat ggactttatc tggcgaaaca ccaagtgcca gttgttccac catttccagc
     2701 gacaccattt cgcctgcaat tttggcaaag cgttttgcgc ggccctgaat ctgcacaaag
     2761 ccctgctcgt caaaacgcac aatatcgcca gtgtcatacc agccgcgctc catttcgccg
     2821 cgaacattct cggcggtggg cacttccagt acacctggct tctccacccg cagatagccg
     2881 ttcattatgt tcggcccttt cagttgcagg cgtccgccct cttcgatacc agggaccgac
     2941 aacaggcgtg catccattcc tggtagaata cgccctaccg taccgggttt cgccgccatc
     3001 ggtacgttga tagaaacgac aggcgcgcat tcggtcacac cgtagccttc aaggatgcgc
     3061 aggccaaatt tatcctgcca aagctgtttg gtactttctt gtagtttttc tgcgcctgcc
     3121 accacatagc gtagacgata gaagtcatac gggttggcga aacgcgcgta gtgaccgagg
     3181 aaagtcgagg taccgaacaa cacggtgcaa ctgcggtcat acaccaagtc cggcacaatg
     3241 cggtaatgca gcgggcttgg ataaaggaac acttctgcac ctgtaagcag tggcgtaaac
     3301 aggcctaccg tcagcccaaa ggagtgaaac agcggtaatg ccgacataaa gcgatcgttg
     3361 gtggtgaagt cggcaatcgt tttaatctgc tcgacgttcg ccagaatgct tttatggcta
     3421 tggacgacgc cttttggatg gccttcagaa ccggaggtaa agaggatcag cgcctcttct
     3481 tccggctgct gttttacctg cgccaggcgc ggcatcagca aatgagcgaa gatccatact
     3541 ttgtcggcag tggtgacatc tgcttttaaa tcttccagat agacccagcg cacctgagta
     3601 agttgctccg gcagatgcca gagtttgcct ttatcgagaa actggcggga agtgaagatg
     3661 gttttgattt cagccgccgt aatagcactg gtcagccctt ttaccccggc agtgtagttc
     3721 atcattgcgg gaatgcggcg acgcgcgatg gccccaaaaa tcactgccgc actgatgcct
     3781 gcattgggca gcattaagcc gatgcgttcg ccttcaacac tgtatttttc aaggatgcgt
     3841 ccaacaaaca gcgttttcgt aagcaatttg cgataggagt ctggggtaaa gttgacgtct
     3901 tcgacacatt tcttcccggc tccgaagcgg tacattgcac tcagtaaaga ttcgtacaga
     3961 gtttcacgcg ggcgcaccgc catacgcgct tccatcatta tttgatgcag catttcgcca
     4021 gcgattttgc gacggtcacg ggcacgcggc gcatccggca tcgccacctg cgttggaggc
     4081 aaaatatgca gagtaatttg cgggaacaag cgacgtttaa ccagaccttt caggcggctg
     4141 aagtgcgtaa gttccgcccc ttcaatacgc acaggaataa ccgttgcacc agacttcgcc
     4201 gcgacaaaac ccgcgccatc gtagattttc atcagcgagc ctgtagtggt gatgcgtcct
     4261 tcagggaaaa tcaccactgg tcggccctgt tccaccagac gtaccagatg tttaatagcc
     4321 ataggttgcg tcgggtcgag aggaacaaag tcgataaatg atttcagcca acgcatatac
     4381 cactgttggc ttattgaggt gtaaacggca aacactggac gcacaggtaa aaacagtcca
     4441 agcaaaatgc catcaataaa agagacgtga ttaggcgtaa ttagaacgcg ctcgcccttc
     4501 agtgcctggg tgtcacccgt aacgcgaacg cgatacaaaa cacggcacaa atttcgaaaa
     4561 aagctaaaaa gcattccaac tccctttgct ctgattcagt aaaagcgaat ggagggagat
     4621 tacacgagat aaagaacgcg agcgacagta aattaggtgc gaaaaaaaac ctgcgcatcc
     4681 gcgcaggttg gtgcaagaga cagggtacga agagcgtacc gaataatctc accaatcaat
     4741 acctctggga tcaccacttt agcaacctga agccaaacgc caccagcggt caatcgcaac
     4801 agcgtttcgc aaagtgtaac caaaggtttg caatctcttt ttttgtctta cttatttgcg
     4861 tttggctcac attcccacga tgaaaacacg ccaccccttg aaccaacggg cgttttccgt
     4921 aacactgaaa caatgtaagc gtttacccac taaggtattt tcatggcgac cataaaggat
     4981 gtagcccgac tggcaggcgt ttcagtcgcc accgtttccc gcgtcattaa taattcaccc
     5041 aaagccagcg aagcttcccg gctggctgtg catagtgcaa tggagtctct tagctatcac
     5101 ccgaacgcca acgcccgtgc gctggcgcag cagaccactg aaacggtcgg tctgatcgtt
     5161 ggtgatgttt ccgatccgtt tttcggtgca atggtgaaag cggtcgaaca ggtggcttat
     5221 cacaccggta atttcttatt gattggcaac ggttaccaca acgaacaaaa agagcgtcag
     5281 gctattgagc aactgatccg ccatcgctgt gctgcgttgg tcgtccatgc caaaatgatc
     5341 ccggatgccg atttagcctc attaatgaaa caaatgcccg gtatggtgct gatcaaccgt
     5401 atcctgcctg gctttgaaaa ccgttgtatt gctcttgacg atcgttacgg tgcctggctg
     5461 gcaacgcgtc atttaattca gcaaggtcat acccgcattg gttatctgtg ctctaaccac
     5521 tctatttctg acgccgaaga tcgtctgcaa gggtattacg atgcccttgc tgaaagtggt
     5581 attccggcca atgatcggct ggtgacattt ggcgaaccag acgaaaacgg cggcgaacag
     5641 gcaatgaccg agcttttggg acgaggaaga aatttcactg cggtagcctg ttataacgat
     5701 tcaatggcgg cgggcgcgat gggcgttctc aatgataatg gtattgatgt accgggtgag
     5761 atttcgttaa ttggctttga tgatgtgctg gtgtcacgct atgtgcgtcc gcgcctgacc
     5821 accgtgcgtt acccaatcgt aacgatggcg acccaggctg ccgaactggc tttggcactg
     5881 gcggataatc gccctctccc ggaaattact aatgtattta gtccgacgct ggtacgtcgt
     5941 cattcagtgt caactccgtc gctggaggca agtcatcatg caaccagcga ctaaccgcag
     6001 ttaaagcaat tccagcgcca gtaattcttc gatggtctgg cgacggcgaa tcaaccgcgc
     6061 ctgaccatta tcaaacagaa cttctggtaa cagcggacgg ctattgtagt tggatgacat
     6121 tgatgcgcca tatgcccctg tatcatgcag taccagataa tcacctgcct tcacttccgg
     6181 caaggcgcgg gtttcaacat ttcccccttc ctgctgggta aagacatcgc ccgattcaca
     6241 taacggcccg gcgacgacgg tttccaccgt tggcgcgtgt tccagagaac gaccatcagc
     6301 tgccagggca ctgatatggt ggtaactacc gtacattgcc gggcgcatca gatcgttgaa
     6361 cccggcatca accagcacaa agtggcggct acccatttgt ttgacgctcc gcacctgagt
     6421 aattaatacg ccagactgcg ctaccaggaa gcgacccggt tcaatttcca gtttcacagg
     6481 gtggcccaaa tggcgagcga tttgctcacg cgcggcattc cacagaccat aataatgttc
     6541 ggtatcaacc gcctcttcac cctgttgata aggaatagaa agcccaccgc ccgcagaaat
     6601 agcctgtaaa tcctgaccga attcgatgac ctgacgcacc atagcaccac acacctgctc
     6661 cagatgggca taatcaacgc cagaaccaat gtgcatgtga atgccgacta gctgcagatg
     6721 atgacgttgt atcacgtcca gtgcggcggg cagatcggtg taccagatac cgtgcttgct
     6781 gttttcgcca ccggtattgg ttttttggct atgtccgtga ccaaaccccg gattaacgcg
     6841 cagccatacc cgatgccctg gcgaaacctg gcccagttgg tcgagcatat caacagaacc
     6901 cgcattcacc ggaatttgca attcactgac gcgttcaagc gtcgcctgat cgataacatc
     6961 tgccgtaaaa acaatatcat cggggtgcgt ttgcggattg taacccgccg ccaacgcacg
     7021 ctctatttcg cctaacgaga cggaatccac tttcacgccc tgctcacgca ttaagcgcaa
     7081 aatatgaata ttggaacagg ctttctgtgc aaagcgcacc acttcaaact gtttcagcgc
     7141 tgctatctgc cgacgaataa tttgcgcatc gtagacccac accgggcagc caaattcagc
     7201 gggcaaacgc agcagatttt cggcggtgag atcggtatcg gtgctgaaca gtgaatgtgg
     7261 cataacaaac tccagatgag tgctttttta tgattacgcc acatcataaa aagaataaaa
     7321 aatatcgatt tatgtcgaat ctatgcaaaa atgatatgga ttaccggatt gcgagagagc
     7381 gctaatggcc gccgttaact tacgtcatat tgaaattttt catgcggtaa tgaccgccgg
     7441 aagcctgact gaggcagcac acctgctaca cacctcacag ccaaccgtca gccgcgaact
     7501 ggcgcgcttt gagaaggtga tcgggctgaa attgtttgag cgcgtacgtg gacgattaca
     7561 tcctaccgtg caaggactgc gtctgtttga agaagtgcaa cgatcctggt acggactgga
     7621 tcgcattgtc agcgccgcag aaagtctgcg cgagtttcgc cagggagaac tgtctattgc
     7681 ctgcctgccg gtcttttcgc aatctttttt accgcagctc ctgcaaccct ttctggcacg
     7741 ttatcccgat gtcagcttaa atatcgtgcc ccaggaatca ccgctacttg aagagtggct
     7801 ctcggcccag cgtcatgatt taggactcac tgagacactc catacgcctg cgggaacaga
     7861 acgtaccgaa ttactctctt tagatgaagt gtgtgtgtta cctccgggtc atccgctggc
     7921 ggtaaaaaag gtattaacgc cggatgattt tcagggtgag aattacatca gcctttcccg
     7981 tactgacagc tatcgccagt tgctggatca gctatttact gaacatcagg ttaaacgacg
     8041 catgatcgta gaaacccaca gcgccgcgtc agtctgcgca atggtacggg cgggggtagg
     8101 tgtttcggtg gttaacccgc tcaccgcact agattatgcg gcaagcggtt tagtggtgcg
     8161 gcggttcagt attgcggttc cgttcaccgt cagcctgatc cgccccctgc accgcccgtc
     8221 atcagcgctg gttcaggcgt ttagtgggca tttacaagcg gggttaccga aactggtcac
     8281 ttctcttgac gctattttgt cgtcagctac gacagcataa aagcgacagc atcctcggca
     8341 tggatcgacg cggtatcaaa cacaggcaaa acactgcgct cttctggcac cagtaaacca
     8401 atttctgtgc agccaaaaat gacgccctgt gcgccctgtt ctgcaaggcg agcaatcact
     8461 tgcgcataat aagcgcgtga cgcttcggta aatttcccca gacacagttc ttcaaaaata
     8521 atctgattaa ttttcgcccg ttcatccgct tcaggaataa gacaattgat ggaaaattgt
     8581 tccgtcagcc gcccgcgata aaaatcctgt tccatggtgt aacgcgtacc cagcagcgcc
     8641 acacgagtca ttccggcccc ggtaattgca cgtccggtgg catccgcaat gtgtaaaaaa
     8701 ggcagagtgc aacgtgactc aatggcatcc gccactttat gcatcgtatt ggtacataac
     8761 acaatacctt ctgcgcccgc ccgctgtaag ccaagcgccg cctcagccag aatgtccccg
     8821 gttttatccc attccccgcg acgctggcac tcttctattt catgaaaatc gacgctatgt
     8881 agcagcattt gcgcagagtg aagcccacca agccgctgtt taatgccttc atttatcaga
     8941 cgatagtaag gaatggtgga ttcccagctc attcctccca gcaaaccaat tgttttcatt
     9001 cctctctcct gatgtgtgtt accgcaacaa ggctgaataa ggaacgagac aaacgcctca
     9061 acggccaggt gcccaatctc tattaacgaa aaaagggccg gatgtacagc acatccggcc
     9121 cgtgaaatca gacgccgata tttctcaact tctcgcctgc catcagtttg cgttcgatat
     9181 gttccagcgt gacatttttg gtttccggaa tgagccagaa agtaatgccc acaaacgcaa
     9241 tgttcaacgc agtgtagagc cagaacgtac cggcagcgcc aatgctatca agcagtgtca
     9301 ggaaggccgc gccgataatc atattcgaca cccagtttgt cgtcgtcgaa caggtaatac
     9361 cgaaatcgcg gcatttcagc ggctgaattt cagagcacag gatccacacc actggcgcgg
     9421 cgctcatcgc ataaccggca atacacatca tcgtcatgcc aacagagagc caggacaagc
     9481 cactggaagc cgtaccgtta tcaaactgca tcaggcaata gcccagcacc agagtgccta
     9541 acgccatcac gctgaaacca attttcagag ccggtttacg acctgcttta tctaccgtaa
     9601 agaccgcaat gaaggtcgcg aacataaagg tcagtcccac gaccagagtc gcaatcatct
     9661 gttgttctgt ggtcgtaaag cccgccattt tgaagatacg cggcgcgtag tacatgatga
     9721 tgttcatacc ggtaaactgc tgcatcgcct gcaacaacat accgagaaac acagcacgac
     9781 ggacgttacg gttgatctta aacagtgccc aaccgccctg ttttaacttc aggctttcac
     9841 gaatttcgtt gagttcttct cgcgcttttt ccgacgtatc gcgcagcata cgcaacactt
     9901 cttccgcctc aatatgacgc cccttttccg ccagccagcg cgggctattt ggcaggaaga
     9961 ccaccagaat aatcagcaga actgctggta aagcaagaac cccaacattg cgcgccagtt
    10021 accgctataa ctgaacgctg tatcggataa aaacgccagc acgatgccga gtgtgaccat
    10081 caactggtac atactgatca tcttaccgcg aacgtacttg ccatttcaga aagatacaga
    10141 ggagcggtgt aagacgcgat cccgacagca atgcccagca ccacacgagc ggcgattaac
    10201 atctctacgc tggtcgcaaa agcggaccct atagaaccga gtacaaacag gatggcccca
    10261 ggcggaacga cagccaacca ttaaacagcg caccaattgc tgcgccgagc atcatgctac
    10321 taaccaccca ttcctgcaaa cgactggtca gcacaaagtg atcggtaatg aacggcaacg
    10381 ctccggcgat tacgccgata tcaagaccaa ataacaatcc tgcgaccgca gcagctaccg
    10441 aaacaaacat attcatacgc cgcgtatcac gcaaagaacg tggcgttaaa gcagattccg
    10501 tattgatagt aaccattttt tcctgccagc agagagtaag acattgtgaa aaaatacgtg
    10561 aacaattcac acaggtgtca ggtcggaaac agcataaata tggattaaat tgctgcgaca
    10621 tgccgttatg tgatggatat tccaatttta aaattaagtt gaattattga gattattatt
    10681 aaccacctaa ttttaaagta gataaaattc ataaagttca ttaattgata attgatatgg
    10741 attatttcat aaccatgata tggattatga tgatctagag atataaaaaa ccctgccatg
    10801 cggcagggtc ataaaagtaa gaggaatgaa ttaacgcgcc agccaaccgc catccacagc
    10861 aatggtataa ccatttacat aatctgaagc gctggaggca agaaacacta ccggccccat
    10921 caggtcactc ggcagtcccc aacggccagc tggaatgcga tcgagaattt ccgcgctacg
    10981 ttgttcatct gcccgcagtt gttgggtatt gttggtcgcc atgtaacccg gagctatcgc
    11041 attaacatta atgttgtgtt tagcccattc gtttgccatc aatcgcgtca cacccatcac
    11101 tccgcttttt gatgcggtat aagaaggcac acggatcccg ccctggaagg agagcattga
    11161 cgcgatattg ataatcttgc cgccattgcc ttgcgcgata aagtgtttcg ccgctgcctg
    11221 agacatgaag aatacgctct tgatattcag gttcatgaca tcgtcccagt ctttttcgct
    11281 gaactcgaga gcgtcttcgc ggcgaatcaa tccggcgtta ttcaccagga tatcaatatg
    11341 accaaattca gctaccgcgc gatccagcag tccaggaatg ccatcaatct ttcgcagatc
    11401 ggcggtcagg cttaaaaaac gacgccccag cgccgtgacc tgctcgatgg tttcagtcgg
    11461 ttcaacgatg ttaatgccaa caatgtcaca gcccgcttgc gccagcccca acgccatccc
    11521 ctggcccagc ccagtatcac aaccagtgac gaccgcaact ttaccttgga gagaaaatgc
    11581 acttaaaatc ataacaacac cttattcgtt tatgcccaca actagcgcaa atctttaacg
    11641 gccacatggt ccatatcatt aaagacctgg ttttcaccga ccatgcccca gataaaggta
    11701 taagctttgg ttccgacgcc ggaatggatc gaccagctcg gggagatcac cgcctgctcg
    11761 ttatgcatca caatgtgacg cgtttcttgc ggctgcccca tcatgtggaa aacgcaggcg
    11821 tcatcatcca tattgaaata gaaataaact tccatccggc gctcgtgggt gtgacacggc
    11881 atggtgttcc acaagttacc tggagccagc tccgtcagcc ccatactcaa ttgacaggtt
    11941 tccagtacat ccgggacaaa atatttgtta atcgtgcgac ggttactggt gaggttatcg
    12001 cctaacgtga ctggagatac ttcgtccggt gtgacttttt tggtgggata cgtcgtatgc
    12061 gcgggtgcgc aattgtaata aaacttcgcc ggagtggcgg tatcaatact ggcaaagaca
    12121 acttcttttg cacctttacc aacatacagg gcgtcgcggt gaccgatttc atagcattga
    12181 ccatcgacag taatcgtacc ggcaccgcca atattgataa cacctaactc gcgacgttca
    12241 aggaaatagc ttacgccgag ttgtttacca acttccccgc caacggaaac cgttttagtt
    12301 atcggcataa tgccgccaac aataattcgg tcaatgtggc tgtaaaccat ggtgtactca
    12361 tcggcgacaa ataccttttc aaccaaaaat tcattacgca gcccttgggt atccagcgtt
    12421 tttgcgtgcg caccgtggat gctctgtctt acgtccacat caacctccga taatcagtga
    12481 agcgtgaaaa tagaaaacga tagtgaaaca ctgtttcgtt ttctctataa agggatgata
    12541 tgcccatggg ctagggtttt caattaaaaa taaaacaatg ttttattttt aatttaagga
    12601 ttaaaaaaag tgcagtgtcg atcacgaata tgtcatgaga atggaaaaat gagggagccc
    12661 agaaaatgta tgtcgacttg ccagccatca ttattcaaca gattaatgac ggcagatatt
    12721 tatcggtaag gaggaaatgc agagctactc gtcacgttca atggtcaatg ccacgcccta
    12781 gcccccgccg atacaaagcg ttgccagccc tttgcgggca ttacgtttca ccatttcatg
    12841 aaccagagaa accaggattc ggcaaccgga agcgcctatc gggtgaccga gtgcgatcgc
    12901 gccaccattg acattgaccc gacgctcatc ccactcaagc atcttgccaa ccgaaagcgc
    12961 ctgtgcagca aacgcttcat tagcctcgat aagatcgaca tcagccaact gccagcctac
    13021 acgctccagg caacggcggg tcgcatacac cggcgcaatt cccatcaatg ccggatctac
    13081 accaacgctg gcaaatgcgc ggatgcgggc cagcacgggt aaattcaacg ctcgtgcttt
    13141 ggcttcgctc atcatcatta ccgcagctgc gccatcgttt atggatgatg cattccccgc
    13201 ggtcacagaa ccgagactat caaatgaagg atttaaacgg gctaaacctt ctgcactggc
    13261 gtcagtgcgt ggctgttcat cggtatcaac aaccaacgtc tgaccgttac tttggatcat
    13321 taccgggacg atctcatctt taaatcgtcc ggcgtcaatc gccgctcgcg ctttttgttg
    13381 cgagctaagt gcgtaagcat cctgcaactg acggctgatg ccatattcgc gagccagatt
    13441 ttcggcggtg acaccaatat gataatcatt gaaggcatcc cacaacccat catgcaccag
    13501 actgtcaacc aactggctat tgccaagctg tgcaccggtg cggctatcag tcagaacatg
    13561 tggtgcgcgg ctcatgtttt cctggccacc ggcgatgaca atatcagcct cgccacactg
    13621 tatcgcctga gtagccagat gcagtgcttt aagaccggaa ccgcaaacgt cattaatcgt
    13681 gattgcagaa acgctgttag gaagaccacc tttaatagcc gattgccttg ccggattctg
    13741 ccctgcccct gcagtcaaca cctgaccaag aattacttca tccaccgcat atgcaggaac
    13801 gccggtacgt tctattaacg ctttcacgac cagactacca agttccacgg cggaatgacc
    13861 cgctaacgca ccacgaaagc agccgatagg tgtccgtaac gccccgacaa ccacaacgtc
    13921 tttcatcaca acctcgcacc aattaacagt gcaatagtaa atgattgtta attacagtta
    13981 tcttaattgt ttaaaaaaag tgatttttat cacaaaggaa atatgcctga gcagtagtca
    14041 gagtcataac tggcacgtaa ggtttgcaac cactaaccca ccaatagatg ggtagatagg
    14101 gcgttaatct cccatactta acttggttta tggtaaattg ccctccattt tgtttaattt
    14161 gtagatgata cgttcagata atgtctaata tttggtctaa agaagaaact ctgtggagtt
    14221 tcgcgctcta cggcacagcc gttggtgcag gcacgctatt ccttcctatt cagttaggtt
    14281 cggcaggggc tgtggtcctg tttattactg ctctggtcgc ctggccttta acatattagc
    14341 cacataaagc cttatgccag ttcatcctct catcaaaaac atcagcaggt gaagggataa
    14401 cgggcgcggt aacacactac tatggcaaga agattggtaa tctgattacc acgctgtact
    14461 tcatcgcctt ttttgtcgtc gtgttgatat atgcagtggc aattaccaac tcacttacgg
    14521 aacagctggc aaagcatatg gttattgatc ttcgcatccg tatgttggtg agtctgggtg
    14581 ttgtattaat tctgaatctc atttttctga tgggacgtca tgccactatt cgggtaatgg
    14641 gatttttggt attcccattg attgcctatt tcttatttct ttccatttac cttgtcggta
    14701 gttggcaacc tgatctatta acaacccagg tagagttcaa tcagaatacc cttcaccaga
    14761 tatggatatc gattcccgtg atggttttcg cctttagcca tacgcccatt atttctacgt
    14821 ttgccataga cagacgtgaa aaatatggcg aacacgctat ggataaatgc aaaaaaatta
    14881 tgaaagtcgc t
//
LOCUS       DADMGC010000102        14685 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.102, whole genome shotgun sequence.
ACCESSION   DADMGC010000102 DADMGC010000000
VERSION     DADMGC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.102"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            116..1417
                     /gene="rstB"
                     /locus_tag="JNZ73_003004"
     CDS             116..1417
                     /gene="rstB"
                     /locus_tag="JNZ73_003004"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HAY8733217.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            1493..2422
                     /gene="tus"
                     /locus_tag="JNZ73_003005"
     CDS             1493..2422
                     /gene="tus"
                     /locus_tag="JNZ73_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HAY8733218.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(2419..3822)
                     /gene="fumC"
                     /locus_tag="JNZ73_003006"
     CDS             complement(2419..3822)
                     /gene="fumC"
                     /locus_tag="JNZ73_003006"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAY8733219.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSIKAGR"
     gene            complement(3965..5611)
                     /locus_tag="JNZ73_003007"
     CDS             complement(3965..5611)
                     /locus_tag="JNZ73_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAY8733220.1"
                     /translation="MSNKPFHYQSPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            5810..6985
                     /gene="manA"
                     /locus_tag="JNZ73_003008"
     CDS             5810..6985
                     /gene="manA"
                     /locus_tag="JNZ73_003008"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAY8733221.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELGFPIPVDDFAFSLHDLRDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANKSPVTVKGHGRLARVYNKL"
     gene            7086..8594
                     /locus_tag="JNZ73_003009"
     CDS             7086..8594
                     /locus_tag="JNZ73_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY8733222.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8820..10085)
                     /gene="uidC"
                     /locus_tag="JNZ73_003010"
     CDS             complement(8820..10085)
                     /gene="uidC"
                     /locus_tag="JNZ73_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HAY8733223.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYVAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSIRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(10124..11497)
                     /gene="uidB"
                     /locus_tag="JNZ73_003011"
                     /pseudo
     CDS             complement(10124..11497)
                     /gene="uidB"
                     /locus_tag="JNZ73_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            complement(11494..13305)
                     /gene="uidA"
                     /locus_tag="JNZ73_003012"
     CDS             complement(11494..13305)
                     /gene="uidA"
                     /locus_tag="JNZ73_003012"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAY8733224.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHGFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(13693..14283)
                     /locus_tag="JNZ73_003013"
     CDS             complement(13693..14283)
                     /locus_tag="JNZ73_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY8733225.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(14512..>14685)
                     /locus_tag="JNZ73_003014"
     CDS             complement(14512..>14685)
                     /locus_tag="JNZ73_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8733226.1"
                     /translation="WSHYPEIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQ
                     ILTVSGGGVQELN"
BASE COUNT         3686 a         3820 c         3494 g         3685 t
ORIGIN      
        1 atacatccgt ctgttccgga cattatcttc caaacaaggt cttatccagt tggcggttta
       61 cattgctcca cagtcgtgaa ttaatacgaa tagtattctg ttgagggcga tgccaatgaa
      121 aaaattgttt atccagtttt acctgttatt gtttgtctgc ttccttgtga tgtctctgct
      181 ggttgggctg gtgtacaaat ttaccgccga acgcgcgggc aaacagtcgc tggatgattt
      241 gatgaacagt tcgctgtatc tgatgcgcag cgaattgcga gagatccccc cacacgactg
      301 gggtaagacg ctgaaagaga tggatttaaa tctctctttc gatctgcgtg tcgagccact
      361 gagtaaatac catcttgatg atatttccat gcaccgactg cgtggcggcg aaattgtcgc
      421 cctggacgat cagtacacgt ttttgcagcg tatcccgcgc agccactacg tgctggcagt
      481 tggtcctgtt ccttatcttt attacctcca tcaaatgcga ttgctggata tcgccctgat
      541 cgcttttatt gctatttccc tcgcctttcc ggtgtttatc tggatgcgtc cgcactggca
      601 ggatatgtta aaactggaag cagcggcgca acgatttggc gatgggcatc tcaatgaacg
      661 tatccacttt gatgagggtt cgagctttga acgacttggt atcgcattta accagatggc
      721 ggacaatatc aacgccttaa ttgccagcaa aaaacagctt attgacggta tcgctcacga
      781 actgcgaaca ccgttagtgc gcctgcgtta tcgactggag atgagcgata acctgagcgc
      841 cgccgaatcc caggcgttga atcgtgatat cagtcaactt gaagctttaa ttgaagagct
      901 gctgacttat gcccgactcg atcgcccaca aaacgagctt catcttagcg aaccagacct
      961 gccgttgtgg ctgtcaacgc atctggcaga tattcaggca gtaacgcccg ataaaacggt
     1021 acggataaaa acgctcatgc aaggccatta tgcggcgttg gatatgcgct taatggagcg
     1081 cgtgctggat aatttgctca ataacgccct gcgctactgc cattcaacgg ttgaaaccag
     1141 cctgctactg tcggggaata gagcgacatt aattgttgag gatgatggcc cagggattgc
     1201 cccagaaaac cgcgaacata tctttgaacc ttttgttcgc ctcgatccca gccgggatcg
     1261 ctcaaccggc ggctgcgggc tggggctggc aattgtccac tctatagcac tggcaatggg
     1321 cggtacggtt aattgtgaca tcagcgaact gggtggtgct cgcttctcgt ttagctggcc
     1381 gttatggcat aacatcccgc aatttacctc tgcctgacac tacgcgcacg atggtcaagt
     1441 cacaacgact gtgctataaa ataagtatgt tgtaactaaa gtggttaata ttatggcgcg
     1501 ttacgatctc gtagaccgac tcaacactac ctttcgccag atggaacaag agctggctac
     1561 atttgccgct catcttgagc aacacaagct attggttgcc cgcgtgttct ctttgccgga
     1621 ggtaaaaaaa gaggatgagc ataatccgct taatcgtatt gaggtaaaac aacatctcgg
     1681 caacgacgcg cagtcgctgg cgttgcgtca tttccgccat ttatttattc aacaacagtc
     1741 cgaaaatcgc agcagcaagg ccgctgtccg tctgcctggc gtgttgtgtt accaggtcga
     1801 taacctttcg caagcagcgt tggtcagtca tattcagcac atcaataaac tcaagaccac
     1861 gttcgagcat atcgtcacgg ttgaatcaga actccccacc gcggcacgtt ttgaatgggt
     1921 gcatcgtcat ttgccggggc tgatcaccct taatgcttac cgcacgctca ccgttctgca
     1981 cgaccccgcc actttacgct ttggttgggc taataaacat atcattaaga atttgcatcg
     2041 tgatgaagtc ctggcacagc tggaaaaaag cctgaaatca ccacgtagtg tcgcaccgtg
     2101 gacgcgcgag gagtggcaaa gaaaactgga gcgagagtat caggatatcg ctgccctgcc
     2161 acagaacgcg aagttaaaaa tcaaacgtcc agtgaaggtg cagccgattg cccgcgtctg
     2221 gtacaaagga gatcaaaaac aagtccaaca cgcctgccct acaccactga ttgcactgat
     2281 taatcgggat aatggcgcgg gcgtgccgga cgttggtgag ttgttaaatt acgatgccga
     2341 caatgtgcag caccgttata aacctcaagc gcagccgctt cgtttgatca ttccacggct
     2401 gcacctgtat gttgcagatt aacgcccggc ttttatactg ccgaccatct gttctggccg
     2461 tacccagctg tcaaactcgg cttcgctaag ataccccagc gcaagggccg cagcttttaa
     2521 ggtcagccct tctttatgcg cttttttggc gatctccgcg gctttgtcat aaccaatgtg
     2581 ggtgttaagc gcagtcacca gcatcagcga ttcattgagt aattgattga ttcgctcacg
     2641 attcggttca atacccactg cgcagtgttt gttaaaactt tccatgccat ctgccagcaa
     2701 gcgcaccgat tgcaggaaat tatggatcac catcggacgg aagacgttca gttcaaagtt
     2761 accggaagcg ccacccatgt tgatcgccac gtcgttcccc attacctgac agcagagcat
     2821 ggttaatgct tcacactggg ttgggttcac tttccccggc atgattgagc tgcccggctc
     2881 attttccggg attgagattt caccaattcc gcagcgcggg ccagaggcca gccagcggac
     2941 atcattggcg attttcatca gtgacgcagc caaccctttc aacgcgccgt gcgcctgaac
     3001 cagggcatca caggtcgcaa gcgcttcaaa tttgttcggc gcggtaacaa acggtgcaca
     3061 ggtaatgact gccagttcat ctgctacgcg ccgcgcatac tccggatggg tatttagtcc
     3121 agtacccacc gctgtaccgc caagagccag ttccgctacg tgaggcaggc tgtattcgat
     3181 atgtttgaga ttatgctcga gcatcgctac ccagccggaa atctcctgcc ctagtgttag
     3241 cggcgtggcg tcctgcaagt gggttcgacc gattttgacg atatcggcaa atgcacgcga
     3301 tttttcactc agcgtctgtg tcagggtttt aagctgcgga atgagttgct tgcgcagtgc
     3361 cagcagcgcc gcaacgtgca tcgccgtcgg aaagacatcg ttggaacttt ggcttttgtt
     3421 cacgtcgtcg ttagggtgaa ctttacgttc catcccgcgc acaccgccga gtaattcact
     3481 ggcccggtta gccagcactt cgttcatgtt catgttactt tgcgtgccgg agccggtctg
     3541 ccagatagcc agcgggaatt cgtcgtcatg ctgtcctgcc agtacttcat ccgccgcctg
     3601 ccgaatggcg ctcgctttct cttcagacaa caagtctaaa tcttcattaa cttttgccgc
     3661 tgcgcgcttg gttagcgcca gcgcatgaat cagtgaggtg ggcattttct ccgtcgaaat
     3721 gcggaaatgc tccagcgagc gttgagtttg tgcgccccac agcttatctg ccgggacatc
     3781 aatcgccccc atcgaatctt tttcgctgcg tactgtattc atggcctgct cctcacctga
     3841 ttaatttttt ctttctgttt tgctttcgtt aagcaacttt tagctcactc attatttact
     3901 atttgataac aaatgtttgg tctttcgtgc catgtaaaaa aaccgccccg aagggcggct
     3961 ctgtttattt cacacagcga gtgcactgtg tgagttgtat ctgctggaag aagtcatttc
     4021 ctttatcatc cacaaggata aacgccggga aatcttccac ttcaattttc cagatggctt
     4081 ccattcccag ttccggatat tcaacacatt ccaggctctt aatacttccc tgcgccaata
     4141 cagcggccgg accaccgata ctgccaaggt agaagccgcc gtgtttttta caggcatccg
     4201 tcacctgctg gctgcggttg cctttcgcca gcatgatcat acttccgccc tgcgcttgca
     4261 gttgatcgac ataagaatcc atccgtccgg cggtcgttgg gccaagagaa ccggaggcat
     4321 aaccttccgg cgttttggcc ggacccgcgt agtaaatcgg atgatcttta atgtattgcg
     4381 gcagcccttc accgttatcc atccgctctt tcagtttggc gtgagcaata tcacgaccaa
     4441 cgataatcgt gccgttaagc gataagcgtg tagaaacggg atactgcgac aactgtgcga
     4501 ggatctcttt catcggacgg ttaaggtcaa cgcgcaccgc ttcgccttct cccgctttac
     4561 gcagctcttc agggatatat ttacctggat tatgttccag tttttcgatc cagatcccct
     4621 gacggttgat cttcgctttg atattacggt cagcagagca ggaaacaccc ataccaaccg
     4681 gacaggatgc gccgtgacgt ggcaggcgaa tcacgcggat gtcgtgagcg aagtatttac
     4741 caccaaactg cgcacccaga ccgagatttt gcgcttcgat cagtaattct ttttccagtt
     4801 ccacatcgcg gaacgcctga ccgtgctcat tcccttccgt tggcagttca tcatagtatt
     4861 tcgcggaagc cagtttcacc gttttaaggt tcgtttctgc agaagttcca ccaataacga
     4921 acgcaatatg atacggagga caggccgcgg tacccagcgt gcgcatcttc tcaaccaggt
     4981 aatttttcag tttccccggc gtcagtaacg ctttggtttc ctgatagaga tacgtcttgt
     5041 ttgccgaacc accacctttt gcgatacaga ggaatttgta ttcgtcgcca tcaacggcat
     5101 aaagatcgat ctgcgctggc agattggtgc cggtattcac ttctttatac atatccagcg
     5161 gcgcgttttg cgagtagcgc agattatctt cgatataagt gttatagaca ccgcgcgcca
     5221 gcgccgcttc atcaccacca ccggtccata cacgctgccc ttttttacca acaataatcg
     5281 cggtgccggt atcctgacag gttggcagaa cgcctttcgc cgcgatgtcg gagttacgca
     5341 ggaattgcag cgccacatat ttatcatttt cgctggcctc cgggtcacgc agaatgtcgg
     5401 ccacctgttg ttggtgagcc ggacgcagca taaacgacgc atcatgaaac gcctgacgcg
     5461 ccaacagagt taacgcttcg ggtgcgactt tcaaaatctc ctgcccttca aattcagata
     5521 cgctaacgtg ttcactggtt agcaggtaat actcagtatc atcttttttg agtggaaaag
     5581 gagactgata atgaaagggt ttgtttgaca ttgttctctc acttactgcc tggtttggtt
     5641 atgctctggg cgggtgttcc gttgccctgt taaaagcgag taacaatatc ctacacagtt
     5701 ttttaacaaa aactgagact agtacgactt tttgcggctc caggttactt cccgtaggat
     5761 tcttgcttta atagcgggat taatttccac attaaaacag ggattgatca tgcaaaaact
     5821 cattaactca gtgcaaaact atgcctgggg tagcaaaacg gcgttgactg aactttatgg
     5881 tatggaaaat ccgtccagcc agccgatggc cgagctgtgg atgggcgcac atccgaaaag
     5941 cagttcacga gtgcagaatg ccgccggaga tatcgtttca ctgcgtgatg tgattgagag
     6001 tgataaatcg actctgctcg gagaggccgt tgccaaacgc tttggcgaac tgcctttcct
     6061 gttcaaagta ttatgcgcag cacagccact ctccattcag gttcatccaa acaaacacaa
     6121 ttctgaaatc ggttttgcca aagaaaatgc cgcaggtatc ccgatggatg ccgccgagcg
     6181 taactataaa gatcctaacc acaagccgga gctggttttt gcgctgacgc ctttccttgc
     6241 gatgaacgcg tttcgtgaat tttccgagat tgtctcccta ctccagccgg tcgccggtgc
     6301 acatccggcg attgctcact ttttacaaca gccacatgcc gaacgtttaa gcgaactgtt
     6361 cgccaacctg ttgaatatgc agggtgaaga aaaatcccgc gcgctggcga ttttaaaatc
     6421 ggcgctcgac agtcagcagg gtgaaccgtg gcaaacgatt cgtttaattt ctgaatttta
     6481 cccggaagac agcggcctgt tctccccgct gttactgaat gtggtgaaat tgaatcctgg
     6541 cgaagcgatg ttcctgttcg ctgaaacacc gcacgcttac ctgcaaggcg tggcgctgga
     6601 agtgatggcg aactccgata acgtgctgcg tgcaggtctg acgcctaaat acattgatat
     6661 tccggaactg gttgccaatg tgaaattcga agccaaaccg gctaaccagt tgttgaccca
     6721 gccggtgaaa caaggtgcag aactgggctt cccgattcct gtggatgatt ttgccttctc
     6781 gctgcatgac cttagggata aagaaaccac catcagccag cagagtgccg ccattttgtt
     6841 ctgcgtcgaa ggcgatgcaa cgttgtggaa aggttctcag cagttacagc ttaaaccggg
     6901 tgaatcagcg tttattgccg ccaacaaatc accggtgact gtcaaaggcc acggccgttt
     6961 agcgcgtgtt tacaacaagc tgtaagagct tactgaaaaa attaacatct cttgctaagc
     7021 ttattaaagg cttataacac cttcaggcgg ccagtccgcc tgatttcatt ttatggataa
     7081 tcattatgaa taaatcgctg gtagcggtag gcgtcattgt tgcgctaggc gtagtctgga
     7141 caggcggcgc atggtataca ggcaagaaga ttgaaaccca tctcgaagac atggtcgcgc
     7201 aggcgaacgc gcaactcaaa ctgacagctc ctgaatccaa cctggaagtg agttatcaaa
     7261 actatcatcg cggcgtattc agcagccagt tgcaactgtt ggtgaaaccc attgctggga
     7321 aagaaaatcc gtggattaaa agcggtcaga gcgtcatctt caacgaatcg gttgatcatg
     7381 gtcccttccc gcttgcccag cttaaaaaac tgaacctgat cccgtcgatg gcatcaattc
     7441 aaaccacgct ggttaataac gaagtaagca aaccactgtt tgatatggca aaaggtgaaa
     7501 cgccttttga gattaactcg cgcattggtt acagcggtga ttccagttcc gatatttcgc
     7561 tcaatccact gaattacgag caaaaggatg aaaaagtcgc ctttagcggc ggcgagttcc
     7621 agttaaatgc tgacagagac ggcaaagcca tctccctttc cggggaggcg caaagtggtc
     7681 ggatagacgc agttaacgaa tacaaccaga aagtgcagtt gacctttaat aatctgaaaa
     7741 ccgacggttc cagcacgctg ggaagttttg gtgagcgcgt aggaaaccaa aaactgtcac
     7801 tggaaaaaat gaccatttca gtggaaggca aagaactggc actgctggaa ggcatggaga
     7861 tcagcggtaa atcggatctg gtcaatgacg gtaaaacgat caatagccaa ctggattact
     7921 cgctaaacag cctgaaggta cagaatcagg atctgggcag cggcaagctg actttaaaag
     7981 tcggccagat tgatggtgaa gcctggcatc agtttagcca gcaatataac gcgcaaactc
     8041 aggcgctgct ggcacagcca gaaattgcca acaatcccga actttatcag gagaaagtga
     8101 cggaagcctt ctttagcgcc ctgccgctga tgttgaaagg cgatccggtg attactatcg
     8161 cgccgctaag ctggaaaaac agtcagggtg aaagtgcgct gaatctgtcg ctgttcctga
     8221 aagatccggc aacgactaaa gaagcgccgc aaacgctggc gcaggaagta gatcgttcgg
     8281 ttaaatctct ggatgcgaaa ctgaccattc cggtggatat ggcaactgag ttgatgactc
     8341 aggtagcgaa gctggaaggt tatcaggaag atcaagcgaa aaaactggcg aaacagcaag
     8401 ttgaaggtgc atcagcaatg gggcagatgt tccgtctgac caccttgcag gacaatacca
     8461 tcaccaccag cctgcaatat gctaacggtc agataacgtt aaatgggcag aaaatgtcgt
     8521 tagaagattt tgttggcatg tttgcgatgc cagctcttaa cgttccggct gtaccggcaa
     8581 ttccgcagca gtaattcacg gccaaaacgc cggagaattt tcactccggc gttttcatct
     8641 gaaaacaaac cagagattgc ctgatgcgct acgcttatca ggcctacctt ttcctgcaac
     8701 actttgaatt tatgagtttt tgtaggctgg ataaggcgtt tacgccgcat ccggcataaa
     8761 aaacgcgcac tttgtcaaca atctgaaacg ccggaggttt ttctctccgg cgttatttct
     8821 tacttcagca taaagtcata ctgaatactc attttcacat cccagtaatt caggcgatcg
     8881 cccagcgtac tgccatcgcg cttttcccgc cctttatcgg cagcaagaaa gaaattcatc
     8941 ccttgtaacg gcccttcgct gacccgatag ttcataccac ctaagatacc cgcgccttca
     9001 atccgcacat ctcgtttact ggattcatag ccgcgagtca caatcggtgc aaatgtcagc
     9061 gtaaattgcg gagttacacg gtagttgaca cctaattgcc aggactgcat tccttcacgg
     9121 ttacgatcaa gacttaatga aaagggatag ccaatgtcgg tatccaccac ggcaccattc
     9181 attgcatggc gcaaataacc accactgcca aatacgcttc cccacggcgc attccaggtc
     9241 aattgtccac taaccaacgc cgtttcattg ggctggctgg tattacgact taaaccttcg
     9301 agctgcgcgt aaaagccgag caactcccct ttcaacgtat aattcgggtc gagcggtatg
     9361 cttttctcga gaatactttg tatctgattg gaaacatctt tctgtaccga tgcccccaga
     9421 gaatagcgac tggtatcatc atgccaactg gcggctaaaa aagaacgtgc gcgatgttta
     9481 tcgtattttc ccgtctggct gttatacacc ccttcatcac gaataccggt tgttgaatca
     9541 gtccataaaa atactcgtga acgccacatt ccccacagat caatatttgg tgttaaagcc
     9601 caataaccta atgctccttc agaaactgtc ggtaatgtac gttgcgaact gccagccatt
     9661 aacggaatac gataaggtaa gctaccataa ctataatccg tcccgaagcg acctaattta
     9721 agcaggctat tttcactggg ttttattttt actgccccca gggcaaagcc aaaactttta
     9781 tcaccatcaa gataccaccg gatactcata tcagcacgag cacctaattt ataaacataa
     9841 tacgctccgc cttcaacgcc aataatattg gaataataac cactattgaa atcgagcaat
     9901 cctccctgta cccatgcgta aatatctctt cccgcgccag cactgggctt atcagccctg
     9961 cgcaattcgt ttttcagtct gatgcgtagt cccgcttcat cgtcagccag ttgcgccacg
    10021 taagcagagg taaggccaga ggcagccgtc aggcaaataa cggccatggc cactattttt
    10081 ctcatagttg cacctttgat gttctgatta tttttattga atattaatta gtgatatcgc
    10141 tgattaattg ctgctgcact tttttacgat tatcaatttc aaccacgatt tctttgaatt
    10201 ttttatccgt gagcggataa aaccagataa taacgaatgc cagtagcata aatccgcaag
    10261 gtactaaggc aattgatgtg cggatgccca taataacttc cggcgtttac acctgattgg
    10321 cgatatatcc gcttaatccc aaaataaagg caggaattga acctccgatt gcctgaccgc
    10381 atttacgggt aaatgagaat agtgaatagg tgagcccttc aattcgcacg ccggtcaggt
    10441 attcaccgta ttctacggta tcagcttcca gtgcccacat cacagtcata gtaacgccct
    10501 gaccaattga agcgatggcc aacgcaacca acgccaccgg cagtgaccag acggaaaccc
    10561 agaagaatag cagataaccg caggttccca gcaaagcgcc aatcaggaag gtattctttt
    10621 taccgatcct cgcgaccatc cccggcacaa gcggtgccga tgccacagta ccaaccaggt
    10681 tttgcaccag taccagcaca gtgaacagcc cggtatcatt taacacatag cgcacgtaga
    10741 acaacgacga ggcgctgacc gcgaaggtcg aaatcagcac acacagcgca ccgatgcaca
    10801 acataaacag cgggcgattc cgtttcaggg tttgcaaact gatcttcaat gacggctgcg
    10861 ccacgatacg taccacattt tcacgcgtcg atttgaagca gatgaagtaa agcaccattc
    10921 cggcaatcgc cagcacaatc gtccagaaat ggtataccga caccatctct tccgggctgg
    10981 agttcttaat gctcggtcct atcagaaatg ccaggcatac aaaggtcaat gaagcggcaa
    11041 tcccacgagc cgcgcccaga cgggcgcgag attgtggttg ttgggtcatc gcggtagcaa
    11101 gtgaaccata aggaatattc accaggctgt agcaaagccc gaggcccatg taggtcaaat
    11161 atgcatacac cactttgcta ccatggctcc agtcggtcgg tacccagaat accagcacgc
    11221 tgaagatcat taacggcgca gtaccgaaga gtaaaaacgg gcggaatttt ccccagcggg
    11281 tattcacact gtccaccact cgtccggcaa agacgtcggc gaaggcatca aatacccgca
    11341 ccagtaacag catggtgcct gccgcagcgg caccgacgcc agcgacgtcg gtgtagtaac
    11401 tcaataggaa gagcgccccc attgcgaagg cgaagttatt ggcgacgtca ccgaggctgt
    11461 agccgacgat ggtgcgccag gagagttgtt gattcattgt ttgcctccct gctgcggttt
    11521 ttcaccgaag ttcatgccag tccagcgttt ttgcagcaga aaagccgccg acttcggttt
    11581 gcggtcgcga gtgaagatcc ctttcttgtt accgccaacg cgcaatatgc cttgcgaggt
    11641 cgcaaaatcg gcgaaattcc atacctgttc accgacgacg gcgctgacgc gatcaaagac
    11701 gcggtgatac atatccagcc atgcacactg atactcttca ctccacatgt cggtgtacat
    11761 tgagtgcagg ccggctaacg tatccacgcc gtattcggtg atgataatcg gctgatgcag
    11821 tttctcctgc caggccagca gttctttttc cagtaccttc tctgccgttt ccaaatcgcc
    11881 gctttggaca taccaaccgt aataacggtt caggcacagc acatcaaaga gatcgctgat
    11941 ggtatcggtg tgagcgtcgc agaacattac attgacgcag gtgatcggac gcgtcgggtc
    12001 gagtttacgc gttgcttccg ccagtggcgc gaaatattcc cgtgcacctt gcggacgggt
    12061 atccggttcg ttggcaatac tccacatcac cacgcttggg tggtttttgt cacgcgctat
    12121 cagctcttta atcgcctgta agtgcgcctg ctgagtttcc ccgttgacag cctcttcgct
    12181 gtacagttct ttcggcttgt tgcccgcttc gaaaccaatg cctaaagaga ggttaaagcc
    12241 gacagctgca gtttcatcaa tcaccacgat gccatgttca tctgcccagt cgagcatctc
    12301 ttcagcgtaa gggtaatgcg aggtacggta ggagttggcc ccaatccagt ccattaatgc
    12361 gtgatcgtgc accatcagca cgttatcgaa tcctttgcct cgcaaatccg catcttcatg
    12421 acgaccaaag ccagtaaagt agaacggttt gtggttaatc aggaactgtt cgcccttcac
    12481 tgccactgac cggatgccga cgcgcagcag gtagatatca cactctgtct ggcttttggc
    12541 tgtgacgcac agttcataga gataaccttc acccggttgc cagaggtgcg gattcaccac
    12601 ttgcaaagtc ccgctggtgc cttgtccagt tgcaaccacc tgttgatccg catcacgcag
    12661 ttcaacgctg atatcaccat tggccaccac ctgccagtca acagacgcgt ggttacagtc
    12721 ttgcgcgaca tgcgtcacca cggtgatatc gtccacccag gtgttcggcg tggtgtagag
    12781 cattacgctg cgatggatcc cggcatagtt aaagaaacca tggaagtaag actgcttttt
    12841 cttgccgttt tcgtcggtaa tcaccattcc cggcgggata gtctgccagt tcagttcgtt
    12901 gttcacacaa acggtgatac gtacactttt cccggcaata acatacggcg tgacatcggc
    12961 ttcaaatggc gtatagccgc cctgatgctc catcacttcc tgattattga cccacacttt
    13021 gccgtaatga gtgaccgcat cgaaacgcag cacgatacgc tggcctgccc aacctttcgg
    13081 tataaagact tcgcgctgat accagacgtt gcccacataa ttacgaatat ctgcatcggc
    13141 gaactgatcg ttaaaactgc ctggcactgc aattgcccgg ctttcttgta acgcgctttc
    13201 ccaccaacgc tgatcaattc cacagttttc gcgatccaga ctgaatgccc acaggccgtc
    13261 gagttttttg atttcacggg ttggggtttc tacaggacgt aacataaggg actcctcatt
    13321 aagataaaaa tactggtcaa cctttaatct gattagttaa ccaattctgt gttgcacaca
    13381 gttatctgtg agaaacccat ggaagagaaa tggaaataaa aagaactgac accgcgatca
    13441 aaaaaccaaa aactgcaata cccacagcgt gatatggatc gcattaatct ttaaaacagc
    13501 gttgacagaa gccataaaac aagcaatcga gcgttcgttc gagagtattg ggaacgataa
    13561 actctcatga catatggcta cggtgaatat tttggggggg aattctccga acacggggag
    13621 gataattttt gcacagagaa attacgatat gaaacgcggg cacagttatc accagcccgc
    13681 gaaatgagag gatcaggatg cggttaatat accgccaatc atcgcgcgta atccctgagc
    13741 aaacgacgct tcacgcgcca ggtcttccgc ttcgatatcg gccagtacac cgtaggtcag
    13801 atccagcagt aaacgtgaag tcattgccgt gttgatgtct gggcttatct cgcctttttg
    13861 ctgggcatca cgcatccgct gggcaacaaa ttcagtgatc gtcatatgct tatttttaag
    13921 catggcggcg acctgtgggt tacgcatccc ttccgccata atttcgacca ccagcgcccg
    13981 ttgcccaaag gcttcatggg tgagagagac aatagactcg accatatagt caacaaaatg
    14041 aatcccttca atcggttccc ggaaacgagc cagcgccctc tcctggtcct gtaagataat
    14101 cgcctgaatc aaggcttctt tggagatgaa atggtgatag agcgtcccag gactaatggc
    14161 gcaagattta cagatggctt tcatcgaggc actgtgaaat ccattttctg aaaaaatctc
    14221 tctggcagca ttgaggatcc gggtccgtgt cggttgtgct tcagtctgca tgttatccat
    14281 cattaaacca ttatagttag cgtgaataac tagtttaccg cgttgtcagg tgagcgccta
    14341 tcattgctgt tgaatagtgc gaaggtacac tctattcgta tacttttcac ccagcaattg
    14401 gttaaccaat tgccgtgatt tggctgccag ttgtttagca gagacgttga tgaattgcac
    14461 acgctatccc ggtaaacaac acatacgacc gtttaccggt tcgttagtgt attaattgag
    14521 ctcctgtacc ccaccaccgg agacggtgag aatttgtccg cttacccagc tggctgcagg
    14581 cgagcaaagg aacagcgccg cgttagcaat atcttgcggt tggcccagac gtctgatcgg
    14641 cgtgtgctgt aacatttttt gttcaatttc tgggtaatga ctcca
//
LOCUS       DADMGC010000103        14527 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.103, whole genome shotgun sequence.
ACCESSION   DADMGC010000103 DADMGC010000000
VERSION     DADMGC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.103"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(337..627)
                     /locus_tag="JNZ73_003015"
     CDS             complement(337..627)
                     /locus_tag="JNZ73_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733227.1"
                     /translation="MDATGNTTVTCLSHKMKYKEVACGKQRMHWLALQFVDENNNPVS
                     GLNVQLEYHPRAMAAELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="JNZ73_003016"
     tRNA            complement(1227..1302)
                     /locus_tag="JNZ73_003016"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="JNZ73_003017"
     CDS             complement(1408..2115)
                     /locus_tag="JNZ73_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAY8733228.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2513..4648
                     /locus_tag="JNZ73_003018"
     CDS             2513..4648
                     /locus_tag="JNZ73_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY8733229.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LVIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVVMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /locus_tag="JNZ73_003019"
     CDS             complement(4697..5953)
                     /locus_tag="JNZ73_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HAY8733230.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGTFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="JNZ73_003020"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="JNZ73_003020"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAY8733231.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="JNZ73_003021"
     CDS             complement(7299..7574)
                     /locus_tag="JNZ73_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HAY8733232.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="JNZ73_003022"
     CDS             complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="JNZ73_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY8733233.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAPLPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQMLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPKFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8815..9534
                     /gene="trmB"
                     /locus_tag="JNZ73_003023"
     CDS             8815..9534
                     /gene="trmB"
                     /locus_tag="JNZ73_003023"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAY8733234.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9534..9860
                     /locus_tag="JNZ73_003024"
     CDS             9534..9860
                     /locus_tag="JNZ73_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HAY8733235.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10044..10763
                     /locus_tag="JNZ73_003025"
     CDS             10044..10763
                     /locus_tag="JNZ73_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HAY8733236.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10939..11985
                     /gene="ansB"
                     /locus_tag="JNZ73_003026"
     CDS             10939..11985
                     /gene="ansB"
                     /locus_tag="JNZ73_003026"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HAY8733237.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12102..13109
                     /locus_tag="JNZ73_003027"
     CDS             12102..13109
                     /locus_tag="JNZ73_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HAY8733238.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13165..14466)
                     /locus_tag="JNZ73_003028"
                     /pseudo
     CDS             complement(13165..14466)
                     /locus_tag="JNZ73_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT         3807 a         3476 c         3734 g         3510 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgccggttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct acttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aagtagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg gtcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaactggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctggtgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctctg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggtga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac gtcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agtagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgattcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6721 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaacaaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc
     7801 gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg
     7861 gcaaacttcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     7921 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcatctgt
     7981 ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt
     8041 tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc
     8101 atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc
     8161 catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc
     8221 agtacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tgcccagaga aagcgagaga
     8281 atcgcgcctg cggtggaacg cccgacgccc ggtaacggcg cgacttcttc aaaggtttcc
     8341 gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     8401 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     8461 agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca
     8521 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     8581 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     8641 tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga
     8701 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     8761 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     8821 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     8881 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaattactgg
     8941 ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt
     9001 gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct
     9061 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     9121 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg
     9181 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     9241 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc
     9301 gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa
     9361 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     9421 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     9481 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     9541 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     9601 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg
     9661 atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     9721 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
     9781 cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt
     9841 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
     9901 cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg
     9961 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc
    10021 tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
    10081 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
    10141 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
    10201 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
    10261 cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa
    10321 gccgcgtcga gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca
    10381 gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc
    10441 gcattatcga aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc
    10501 gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta
    10561 atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg
    10621 gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt
    10681 tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag
    10741 ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc
    10801 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
    10861 ttgtaaattg tttaacgtca aatttcccat acaaagctaa gggataatgc gtagcgttca
    10921 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
    10981 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
    11041 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
    11101 gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag
    11161 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
    11221 attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg
    11281 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
    11341 ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg
    11401 gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac
    11461 accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag
    11521 tctgttaact acggtcctct gggatacatt cacaacggta agattgacta ccaacgtacc
    11581 ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg
    11641 aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
    11701 gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc
    11761 gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc
    11821 gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc
    11881 gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa
    11941 accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc
    12001 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttaccctaat
    12061 gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg
    12121 ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
    12181 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
    12241 ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacctggtca gcggaaggcg
    12301 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
    12361 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
    12421 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
    12481 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
    12541 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
    12601 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
    12661 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
    12721 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
    12781 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
    12841 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
    12901 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
    12961 aaaaagggca atatccgtta acgatgaaag tttcagaaat aaacagtaaa aaagtcgcga
    13021 ttttgatgaa aattgacgat atcaaccaag ctaatgaacg ctggaaaaaa gataccgaac
    13081 aactgcggcg caatggggtg attaagtaac tcatgttaag tgcctttgcc ggataatata
    13141 aaaagttatc cggcaaatta ttttttacat tagccctagt agctgtggaa tgaacaagga
    13201 tatttgcgga atgtaagtga tcatcatcaa cgctactagc aatgcagcat aaaatggcag
    13261 caatggtttg attaaatgtt gaatcttcac acccgagata gaacaaccaa caaatagcgc
    13321 actacccacc ggcagtgtta acagcccaat acaaaggttg gccaccatca taataccgaa
    13381 atgtactggg tccattccca gctcttgtgc aatcggcaag aagatcggtg taaagattag
    13441 tacagccggt gtcatgtcca taaagatgcc gacaattaat aagacgatat taatcagcag
    13501 caatatgatc agtgggttat cagaaatccc catcaatgcg tcgctgatca tataaggaat
    13561 atcagcatta gtcattgccc aggacatgcc cacggaaaaa ccgatcaaca gtaatacaat
    13621 cgaagtggta acaaccgact ccagaatcag tttgggtaaa tggcgccatt ttacttcccg
    13681 gtaaatcagc accgagagaa taaatgtgta gacaacggca attgctgacg cttcagttgc
    13741 ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac accagaagca aacttggtaa
    13801 ggcatccaga aacgctttac aggcctgagc aaaggttgct ctttctgaaa gggggtagcc
    13861 acggcgttta gcaataatgc cgcagactat cattaccgca agacccatta agatccctgg
    13921 caaatatccc gccataaaca gcgatgctac agaaacgccg ccagctgtca gggaaaacac
    13981 aattaacacg tttgagggcg gtatcagtaa tccagtaata caagatgaga cgtttacagc
    14041 ggtagaaaat gccggatcat aaccttcttt ggtctgaatg ggattcagtg tgccaccaac
    14101 ggcagcagct gccgcaacgg ctgaaccaga aatggaacca aacatcatat tcgccaggac
    14161 gttcacatga ccaagcgaac caggaacgcg gccaaccatt acctaggcga ggttaatcaa
    14221 acgtatcgca ataccgccgc tgttcatcag tgttccggca aagatgaaaa aggggattgc
    14281 caacagggca aagttatcca gtccattagc cagtcgttgc gaaacagtga ttgccgcgat
    14341 atcccaggga aacatcaaaa gcattgaagc tacagtggca ataccgattg agaacgaaat
    14401 gggcacacca ataaagacca gcacaaaaaa acagccaaac agtgtaatcg cgatataact
    14461 atccattaac tggcctcctg cgcaggttgt ttcagttgtt gaatgctttg attaataaaa
    14521 cacaagg
//
LOCUS       DADMGC010000104        14252 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.104, whole genome shotgun sequence.
ACCESSION   DADMGC010000104 DADMGC010000000
VERSION     DADMGC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.104"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..106)
                     /locus_tag="JNZ73_003029"
     CDS             complement(<1..106)
                     /locus_tag="JNZ73_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HAY8733239.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(147..602)
                     /gene="csgB"
                     /locus_tag="JNZ73_003030"
     CDS             complement(147..602)
                     /gene="csgB"
                     /locus_tag="JNZ73_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HAY8733240.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1356..2006
                     /gene="csgD"
                     /locus_tag="JNZ73_003031"
     CDS             1356..2006
                     /gene="csgD"
                     /locus_tag="JNZ73_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HAY8733241.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2011..2400
                     /gene="csgE"
                     /locus_tag="JNZ73_003032"
     CDS             2011..2400
                     /gene="csgE"
                     /locus_tag="JNZ73_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HAY8733242.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            2425..2841
                     /gene="csgF"
                     /locus_tag="JNZ73_003033"
     CDS             2425..2841
                     /gene="csgF"
                     /locus_tag="JNZ73_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HAY8733243.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSVLQNNSTDF"
     gene            2868..3643
                     /locus_tag="JNZ73_003034"
                     /pseudo
     CDS             2868..3643
                     /locus_tag="JNZ73_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3707..4198)
                     /locus_tag="JNZ73_003035"
     CDS             complement(3707..4198)
                     /locus_tag="JNZ73_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAY8733244.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4300..4854)
                     /locus_tag="JNZ73_003036"
     CDS             complement(4300..4854)
                     /locus_tag="JNZ73_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8733245.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4878..5615)
                     /locus_tag="JNZ73_003037"
     CDS             complement(4878..5615)
                     /locus_tag="JNZ73_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAY8733246.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5670..6608)
                     /gene="ghrA"
                     /locus_tag="JNZ73_003038"
                     /pseudo
     CDS             complement(5670..6608)
                     /gene="ghrA"
                     /locus_tag="JNZ73_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6842..6929
                     /locus_tag="JNZ73_003039"
     tRNA            6842..6929
                     /locus_tag="JNZ73_003039"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6876..6878,aa:Ser,seq:gga)
     gene            complement(7079..7360)
                     /locus_tag="JNZ73_003040"
                     /pseudo
     CDS             complement(7079..7360)
                     /locus_tag="JNZ73_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7642..8646)
                     /locus_tag="JNZ73_003041"
     CDS             complement(7642..8646)
                     /locus_tag="JNZ73_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HAY8733247.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8718..9278)
                     /locus_tag="JNZ73_003042"
                     /pseudo
     CDS             complement(8718..9278)
                     /locus_tag="JNZ73_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9363..9560)
                     /locus_tag="JNZ73_003043"
     CDS             complement(9363..9560)
                     /locus_tag="JNZ73_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HAY8733248.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9572..10063)
                     /locus_tag="JNZ73_003044"
     CDS             complement(9572..10063)
                     /locus_tag="JNZ73_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733249.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(10060..10434)
                     /locus_tag="JNZ73_003045"
     CDS             complement(10060..10434)
                     /locus_tag="JNZ73_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAY8733250.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10481..10855)
                     /locus_tag="JNZ73_003046"
     CDS             complement(10481..10855)
                     /locus_tag="JNZ73_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HAY8733251.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(11018..11239)
                     /locus_tag="JNZ73_003047"
     CDS             complement(11018..11239)
                     /locus_tag="JNZ73_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HAY8733252.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11302..11778)
                     /locus_tag="JNZ73_003048"
     CDS             complement(11302..11778)
                     /locus_tag="JNZ73_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HAY8733253.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11794..12267)
                     /locus_tag="JNZ73_003049"
     CDS             complement(11794..12267)
                     /locus_tag="JNZ73_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAY8733254.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12609..13430)
                     /locus_tag="JNZ73_003050"
     CDS             complement(12609..13430)
                     /locus_tag="JNZ73_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAY8733255.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13530..13703)
                     /locus_tag="JNZ73_003051"
     CDS             complement(13530..13703)
                     /locus_tag="JNZ73_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAY8733256.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13823..>14252)
                     /locus_tag="JNZ73_003052"
     CDS             complement(13823..>14252)
                     /locus_tag="JNZ73_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733257.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3641 a         3494 c         3409 g         3708 t
ORIGIN      
        1 cgccaccacc gtggttaccg ccgccgccgt actgaggaac aacacctgcc agagcgctac
       61 cggagaatac gattgctgca attgctgcta cttttaaaag tttcatgtaa aacccccatc
      121 ggattgattt aaaagtcgaa tggtaattaa cgttgtgtca cgcgaattgc catttgcgac
      181 tgtctctgca ctacaattgc cgttttttga gtaccatact gtgtaatatt tgctttatta
      241 ccagaacctt tctggataat catcgcagta ttaccataag caccttgcga aatactggca
      301 tcgttggcac tgcccgcctg atcaatatat gcaaggttat aatctcctgt ctggtcaatc
      361 tttgcccgat tgctactacc ttcttgcgca acaaccgcca aaagttttga gcctccctgc
      421 cgtaactgag cactattatt agtcccagct tgaccaatta tggctgcctg attaaatgaa
      481 gacttactca attcatttac cgcgaagtta tattctgaat tagctaaatc ataacctgct
      541 gcggctgcaa tcccaggcgc acccagtatt gttaacatca taaataacaa tttgtttttc
      601 atgttgtcac cctggacctg gtcgtacatt taagaaatta aatcatttca acttggttgt
      661 taacgcaacc tgtattttgt taacgctgcg ttacgatgga aagtatgtct gcggaaatat
      721 ttttaataac tcacccgcgc gttgtatttt tctttttttg ttcataccaa agtattaaaa
      781 cccgcgtaaa tacgctgtta tctacgcaaa aaatattttg ttttctctta aatctccgtt
      841 ttccgctatc aaaaatcacc agacagtcat tcttcttgcc cgtcgctgat tgctgcgata
      901 tgtcttgcgc acaagccgtg acaaacctcg tctttttgca gtaaaaaaat tgtccacgga
      961 ggtgtggaga aaaaacaaaa acgttttaca tgacgaaagg actacaccga aatatttttt
     1021 atatgtatta ttagtaagtt agcaccattt gtatgatttt ttaaaattgt gcaataaaaa
     1081 ccaaatgtac aacttttcta tcatttctaa acttaataaa accttaatgt taacatttta
     1141 atataatgag ttacattttg ttacatattt aacacttgat ttaagatttg taatggctag
     1201 attgaaatca gatgtaatcc attagtttta tattttaccc atttagggct gatttattac
     1261 tacacacagc agtgcaacat ctgtcagtac ttctggtgct tctattttag aggcagctgt
     1321 caggtgtgcg atcaataaaa aagcggggtt tcatcatgtt taatgaagtc catagtattc
     1381 atggtcatac attattgttg atcactaaac cttctttgca ggcgacagct ctcttgcagc
     1441 accttaaaca atcgctagca attacaggaa aattacataa tattcaacgt tctctggacg
     1501 atatctcttc aggctctatt attcttctgg atatgatgga agcggataaa aagcttatcc
     1561 attattggca ggatactttg agcaggaaaa acaacaatat caaaatattg ttgctaaata
     1621 cgcctgaaga ttacccgtac cgcgacattg aaaactggcc tcatatcaac ggcgtttttt
     1681 atgccatgga ggatcaagaa cgtgttgtca atgggttgca aggcgtcctg cgtggcgaat
     1741 gctactttac gcaaaagctt gccagttacc tgattacaca ttctggtaac tatcgttata
     1801 acagcacgga atcagccctc cttactcatc gggaaaaaga gatcctgaat aagctgcgta
     1861 tcggcgcgtc taataacgag atcgctcgtt cgttgttcat cagcgaaaat acggttaaaa
     1921 cgcatcttta taatcttttc aagaagatag ccgtaaaaaa ccggacacaa gcggtttcct
     1981 gggcaaacga taacctcagg cgataaagcc atgaaacgtt atttacgctg gattgtggcg
     2041 gcagaatttc tgttcgccgc agggaatctt cacgccgttg aggtagaagt cccgggattg
     2101 ttaactgacc atactgtttc atctattggc catgattttt accgagcctt tagtgataaa
     2161 tgggaaagtg actatacggg taacttaacg attaatgaaa ggcccagtgc acgatgggga
     2221 agctggatca caataacggt caatcaggat gttattttcc agactttttt attcccgttg
     2281 aaaagagact tcgagaaaac tgtcgtcttt gcactgattc aaactgaaga agcactaaat
     2341 cgtcgccaga taaatcaggc gttattaagt acgggcgatt tggcgcatga tgaattctaa
     2401 ataaaaaatt gttcggaggc tgcaatgcgt gtcaaacatg cagtagttct actcatgctt
     2461 atttcgccat taagttgggc tggaaccatg actttccagt tccgtaatcc aaactttggt
     2521 ggtaacccaa ataatggcgc ttttttatta aatagcgctc aggcccaaaa ctcttataaa
     2581 gatccgagct ataacgatga ctttggtatt gaaacaccct cagcgttaga taactttact
     2641 caggccatcc agtcacaaat tttaggtggg ctactgtcga atattaatac cggtaaaccg
     2701 ggccgcatgg tgaccaacga ttatattgtt gatattgcta accgcgatgg tcaattgcag
     2761 ttgaacgtga cagatcgtaa aaccggacaa acctcgacca tccaggtttc ggttttacaa
     2821 aataactcaa ccgattttta agccccagct tcataaggaa aataatcatg cagcgcttat
     2881 ttcttttggt tgccgtcatg ttactgagcg gatgcttaac cgccccgcct aaagaagccg
     2941 ccagaccgac attaatgcct cgtgctcaga gctacaaaga tttgacccat ctgccagcgc
     3001 cgacgggtaa gatctttgtt tcggtataca acattcagga cgaaaccggg caatttaaac
     3061 cctacccggc aagtaacttc tccactgctg ttccgcaaag cgccagggct tacaaaacct
     3121 gcttaacgag cgcaaggtta ttcgtgcggc acaagaaaac ggcacggttg ccattaataa
     3181 ccgaatcccg ctgcaatctt taacggcggc aaatattatg gttgaaggtt cgattattgg
     3241 ttatgaaagc aacgttaaat cgggcggtgt tggggcaaga tattttggga tcggtgcaga
     3301 cacgcaatac cagctcgatc agattgccgt gaacctgcgt gtcgtcaatg tgagtaccgg
     3361 tgagatcctc tcctcggtga acaccagtaa gacgatactt tcctatgaag ttcaggccgg
     3421 ggttttccgc tttattgact accagcgctt gctggaaggg gaagtgggtt acacctcaaa
     3481 cgaacccgtt atgctgtgtc tgatgtcggc tatcgaaaca ggggtcattt tcctgattaa
     3541 tgatggtatc gaccgtggtc tgtgggattt gcaaaataaa gcagaacggc agaatgacat
     3601 tctggtgaaa taccgccata tgtcggttcc accggaatcc tgacagataa aaatgccgga
     3661 attaataatc cggcatttac tgtttaagcg aaagcgtgag tgcccctcac gctttatttt
     3721 tgatttcctg ctcacggtga gccgtctttg cactacgcgc cgccagccac aggccgctgt
     3781 ttttcattgc gtaaccaaat accagcccaa gcgccagcga aggtaacacc agtttccagt
     3841 caccctgccc ggcaaacgtc gcgcatgcgc ctataaacgt ccccggaaca aatgaaagca
     3901 gcagctgttt ggcctgaata cacatcagaa acgcgacaat accggttatg acataaccga
     3961 gaatttccag atgtggtgcc agtgcactac cgtaaataat gaccatcgcc cacaccacgc
     4021 cactaagcag ggttgcagcg gagatcgcca gccctttcag cccaccttgc gggcaagcaa
     4081 agtaggccgt acagcccagg aagcccgccc agcttagtaa acccagggaa acagccaccc
     4141 atccccagat accggagaga atgcctgttg tgattgcaat agagagaagt atgttcatgc
     4201 cgcgcatgat agcagaaaac gcggccatga atgagatcga gcacacattt tatgcaacaa
     4261 caaattatct gttgcagtta tgagtgacga tgatcacatt tactcttcag aatcttcttc
     4321 cagctcgtcc cacattgcac taatggcatc acgggttagc ggtgccatgg tgcgccagaa
     4381 aggcgtggtt gcatgggcct ccactttgcc gaggaacgca ccacaccagg gtaacagata
     4441 ctcactgaac agtgtttcca gtgcttcgct ctcatcttcc gttgactgat cttccagcca
     4501 ggaagccgca agcagcaatg tgccgatgtg atcggctggc gtatccgcca gtggcatccc
     4561 tcgttcggaa agaaaagcgc gcacttccgc ttccgttgcg tcctcaaccc atgcgctacg
     4621 atatggcggc acggcacatt catcgccgat aaacaacgca ttgtaatcgg cagagacttg
     4681 cgtcatatca cagcttttct gtaaacgcgt cagtaactca tcctgctcca gtggccagtt
     4741 cgcagccagt ttcccctcac gaatcagagt aaacagcggc accagtaaag gatcttgcgg
     4801 ttggcggtaa tagagcgaac ccagcacacg acagaggata gaaaactcgt tcattaatat
     4861 atttccatta ctagtaatta aaggtctgca aattccgcaa tcggtgccat accgcgagat
     4921 tcaaggaagt tcagtaagcg gcgcggagaa acattcaaaa tgcgctctgg cggaaaatct
     4981 accgcgtcga ggattttaag acactcttca aattccccca tggtaaacgc ggtgtgagaa
     5041 tccgagccta atgccaccca acctccagca tcacgtaccg ctgcagctac cgcacgacag
     5101 ttgtcttcac tgcccttacg tgagtgtaaa aatgaggaat tattgatttc cagcgccacc
     5161 tgatgtttcg cggctgcttc agcaacggct ttaacatcta tttcatattt cggatttccg
     5221 ggatggctta ttatatgcac attgccgctg gcgatagtgg cgatcatcgc ttgtgtattg
     5281 gtcgctttgt catgtggcgc aaaaaccggc tcatgaaaac cggcaataat cagatccagc
     5341 gagtcaaaca ttttaccgct gcagtcaatt tcaccatcaa catttttaat gttagcttca
     5401 atgccgcgca ggatccctac cccatcaacc actcgcggcc agatacgcat gttaatgaag
     5461 tgccagtgat gcggcgcatc ttccatatcc gggccatgat cggtgatcgc aaaaagttta
     5521 atgccctttt gtttggcctg ggcaatgtaa tcactcaatg tgctatatgc atgtgtgctg
     5581 gcaacggtat gcatatgaag gtcgacggga tacataactc tctcctgtat tcaattcccg
     5641 ccaaggatag caggaatcct gatgctttat tagtagccgc gtgcgcggtc gacttgcccg
     5701 cagaccctct cccctttttc gagctgggca atagtgcgag aaatgtactc cacagcttca
     5761 gcgggacggg taatcgcggc gacatgtggt gttatcgtca cgcgtggatg ttgccagagc
     5821 ggactttcag gcggtaaggg ttcacgatta aaaacatcca gcattgcgcc tttaactttc
     5881 ccgctatcca gcgccgcgag caggtcatct tccacaacat gaacaccacg cgccaggttg
     5941 aggagatacg cgccatcagg taatttttcg agtaattgtt gattaataat gccgacggtt
     6001 tcaggggtat tcggtaacaa attaatcaat acccgacatt ggctcagaaa tgcagacagt
     6061 tcttcccgtc cggcaaagct ttgcacgcca ggccacgatt tacgggttcg actccagcaa
     6121 cgcagcggaa agcgctaggt ttgcagactc tgagcaactt tacagcccag tacgcctgcg
     6181 cccaaaatgc cgatggtaaa atcttcccga tgatattcag gcagcggttg ccaatgcgaa
     6241 ctattttgct ggatgcgata atcatcaaaa cgtcgaaacc aatgcagcac ctgactgaca
     6301 gcatattcct gcatttgctc gcccataccg gtatcttcca ggcgaaaaag tggaacagaa
     6361 gggttcagca tttcagggtg tgcctgtagc ttgctcaaaa tagaatcaac accggccccg
     6421 agtgcgaaca ccgctttaag atcgcgccct gccagcattt caacaggagg atgccagact
     6481 aaaacataat cagcagagtc attatctccg cttttccatg ccctgactct tgcctgagga
     6541 atagctttgc gcagtgcctc aatccaccat tgggtatcga acgttgggtg ataaaagatg
     6601 atatccatac tgactcccga aaagcgttgt gcgaaattta ttcgcactta tcgttatgat
     6661 ctacaaaggc caccagcata acaaatccgt ggtcggtggc aaaaaaagca gatttcgctt
     6721 attaaaacca cacattgatt gaagtttgaa taaacgcgcg attctttcaa aaagtttgtt
     6781 gacctcaggt catgatttcc ctaaattagc gcccgttcca gcaagacagg aacgacaatt
     6841 tggtgaggtg tccgagtggc tgaaggagca cgcctggaaa gtgtgtatac ggcaacgtat
     6901 cgggggttcg aatccccccc tcaccgccag attataagtg catgttattg tttaacatgc
     6961 gcttttttat ttttccttcc agacagtatg ccatcgggta tgccatctga aaccggttta
     7021 tatgccccgc gatcatatct tatcggacaa tttccgatct ttgagcatac tggcaaagct
     7081 atactgccag catatcagga tagtgttttg cgataatatc attcagtctg tcaaccagtt
     7141 ctggttttct gaacgtgata tgcgcggagc ccttcttaaa gtatctgatg ctgaacatct
     7201 catcttcata gctctcttta ccctgaacag actgaatgtg atcatccaga cgaagggtga
     7261 tgtttccccg gttgtcgggg attggtttac cgctgaacag gtgcaacatt ctttccagat
     7321 cggcgagtcg gtcagcctgt tgtccggtga taagatgaaa tctatggtca cccccgtttt
     7381 tgcaacactg atttttgatt aatggttggc ttgcttaaat ctatccggcg tctctgtggg
     7441 caaggatgcc cgcgccccga tgagttccgc acctgataat cctaaaaatc ccctcggctt
     7501 cagtgagcca tttcatctgt taggttttta atcaggtcga tttgccgttc atgtcatcaa
     7561 taatcagtct tcgcaaaacc agaaaggtaa cttgtatctt attgtttaaa ataaactaat
     7621 tgagttatat cagcatctat atcaggcctg atattctttt ttctgagtag tgattgccca
     7681 ggcaattcga gccagtttat ttgccagagc gcaggtaacc acattcgaat gcttcttgtt
     7741 gagttgctcc cgaacccact cagcaagttt tccatgcttg cgttcaagtc gcattatgaa
     7801 tgagcgggca cactgaacca gtaaccggcg taaattttta tcacctcgct tactgatacc
     7861 caacagagtc gattttcctc cagtgctata ctgccgggga acaagtcctg tggaagctgc
     7921 aaaatccctg ctgcatgaga actgtttccc gtcaccaagt tgcgacgata gtaagctggc
     7981 agtaatcgga ccaacaccag gaattgtcat aatgcgctga gcggtatcat cagcgttgat
     8041 tgactgactt aattctgatt ccagctctgc tatttgctca acaaggtaaa agtagtgggt
     8101 atgcaacctc accaaaaggc ggctcagata ttcggggatt tcgtgttcag ccaacacgag
     8161 agacaaccgt tttattacag catcacctat cggcaggctg attccaaatt ccagcaagaa
     8221 accgtggatc tggttggttg ttttcacctt atctctgatg agcgattctc tgacccggtg
     8281 taacgccctc atggcctgct gggtctcagt tcttggctgg acaaaacgca ttgatggtcg
     8341 tgaagccgct tcgcatatgg cttctgcatc aacaaaatca ttcttattac ttttgacaaa
     8401 cgggcgaacg aactgtggag aaatcagctt agtttcatgc ccaaaatcag agatacagcg
     8461 agccataaag tgtgcacccg cacaggcttc cattacaaca actgagggcg gacatgtcgc
     8521 cagaaactgc atcagtttag tacgggtaaa ttttttacgc agaagagcct taccagactt
     8581 gtcttgacaa tgaatgtgga aggagtgctt tccgagatcg ataccaataa gtgtgatgtt
     8641 ctgcatgatg gtctctccgt taaagggatt taccttaaca gcgtactgct gttaaggtgg
     8701 gggtgaccat atcattatcc ccaccggtcc catctcacca gattgttgac gataatttta
     8761 ctgccaaatt tacaggggca attggttttg taattccagt tcagccctct gaagacgttg
     8821 atcacccctc gctcaaacac ctcatcctta ttctggtgca gttgttcaaa tgtgctcaga
     8881 atgttcgctt cgctgatttc cggaaaatta tcgtactcca gtgaccggta ccatgtatca
     8941 cgggcctgcg tatccattaa tgacagcatc ccggaacgtt gcatcaggtc acgccagatt
     9001 tcgcgatcaa gattccgggt aatggctttc attgctgttt cgggtttctc catcagccag
     9061 cagccgcagc gaaaatcctg tttcatggcc cagtccgggg cagttttacc tccgatgctt
     9121 tccgtcagca ccgagatttc agccagttgt cggataagtg tgtcgatttg caacagtgta
     9181 ctctttcgta cagccagaat gtggcgaatg ctggttgagc agataacgtc cgtatgcccg
     9241 ttgaccactt ccggacggga gattgtgata tctgacataa tcgatattcc tttttgcaga
     9301 taaatgaaaa cgccagccct gtgcaggctg gcgtcagagg aatgaagtct ggtgagtgag
     9361 cttcaccggt tctggtgcag aagctgcaga tgacgcaggg tacggacatc ctcccgggcc
     9421 tgttcgatac agggcaacag tgctgctgaa tccgttttat cctcatcgtt gtcaaagata
     9481 attcccgatc cgcagtcgat attgtcctgc agccacgcaa tcagaatatc cagcgctgtt
     9541 tccgtggtta atgatttcat gttgttcctg gttaccagtc gaaagtgggt aaacccggca
     9601 ggcattcagc gctggcatcc agatgaatga gactgacgcc ataacgccgg atgagtgtga
     9661 cgaccagacg acggaacgtt tttgacagcc ccaggcgttt aagccgcaga accgggtatg
     9721 accacgcatc ggtacgtaac agatatccgc tacctgtaaa atgaatccat tctgattcac
     9781 cgaagtcact ggtctggtgt gacagcgagt acaaccaggc gttgtccgtt tccgtgatat
     9841 gtgcggtact gcagcgtatg ccggtaaggg tagcaaatgg tggcgtgcat tctgctgagg
     9901 tctggtcaga ttcatcaaca acacgaaggg agtagccgtt atcgcagacc atgttaatca
     9961 gttcagcgag ctcaatacca tcgacgtcaa cgacaatgcg ccctatgttc agtgcctgta
    10021 cgttaacgct gtcggcttcc agtgtcaggg ccagtttcat catttcgcct ccggatactt
    10081 acccagggta atgttattta ccgttctgta attgtcgcgg gtcatcaggc cggtcgcccg
    10141 gcgagcccgg aggatatcaa tactgtttat taactgagag cggggacagg tgctgaatcc
    10201 cggctggtcg gtacgcacca gcgcgtattt ttccacgaga aaattaactg catcacacag
    10261 tgaaatgcct gcctcaatat gctgctcaat cacacgttca tcgacaaacg gtgtgtcatt
    10321 cagtgtgagg ccataatgct ggtccagcag tcggatgagc agtgtctgcc agatggtgac
    10381 gggagacggg cagcgagata cctcccgtac atgtgtgtcg gataatgttt tcatcttaag
    10441 ttctctgtaa ctggtagtgc taaaggtgag agcttcgggg ttatgaggtg gttgcgggtg
    10501 ctggtgtcgg ataaacagcc agataaacgt aaccacagga gccgagggtg tcggcttcgc
    10561 aggtcagccc ttttgcatac agcgtgacgg tatgctgata tcggggattc agttcaccgc
    10621 tggtgagcat gagttccagt tgtttcatca gcagcggaaa ggcctggtcc agatggtatg
    10681 catccgcatt gctgaaccgg cctctgatac cggcgcggtc tgcaaggtaa tacaggcggt
    10741 taccctcttg caccagacga gcgccaaaac agggcctcac tgtgcaggga agcccccacc
    10801 aggggcggtc gtgattgtca tcgggatgag ttatcccgga gagtttgtct gacacgataa
    10861 aatcctctct gaaaagtggt taagaatggt ttaacgatga caggtcagcc ggcgcgctga
    10921 ccggtttcgg tgaagatgta tgcattgatg agaatggcct catccacagc ggcgtctgag
    10981 gtcatctggt cgtactcgtt tcccgggtga cggaacatca gttcagggtg acactcatca
    11041 gccggacata cgggccaaaa ctgtccttac ggcgttctgc aaacacggca agaacgccgg
    11101 gaatatcctg cacttcacga ccagtatacg cttcagcact gccgtgccag cgatacttac
    11161 cggtacagaa cggaaaaaga cgggatgcag gatgctgttg gtgaatacgc atggcttcac
    11221 cacgggtgat gattctcatg atgtgatacc tctgaagaca gaagataaaa gtgaaaacag
    11281 gtgtgatgtg gttgtgacgg gttaaagcag accatgttcg gcaaaggaga aaacctggtt
    11341 gccaccgact atcagatggt ccggcacccg gatatccacc aggcccagtg cctgtaccag
    11401 acgttcggtg ataagccggt ctgccttact gggtgtgact tcaccggacg ggtgattgtg
    11461 cgccagcacc acggcagcgg cattgtggta cagggcgcgt ttaatcactt cccggggatg
    11521 aacttccgta cggttgatgg tgccggtgaa gagggtttca ccggcaatca gctgattctg
    11581 gttgttcaga tacagcaccc ggaactcttc ccgctccagt cccgccatgt tcagaatcag
    11641 ccattcccgt gccgcacggg tggaggtgaa ggccacgccg ggttcatgaa gatggcggtc
    11701 cagggtttgc agggcccgca gaatgagact gcgctcgccg ggcgtcatgt ttccgggcag
    11761 aaaggaaagc tgctgcattg tccctgcctc cattcagtcg atgatgcgca taatggcgct
    11821 gcattccgga tgctgcaggg cgtaatcccg cagccggtaa taatggaccg tcatggcata
    11881 acactccgta cgacaggcat gatgactgta cgtcatcaga caggcagcaa tgccggcggc
    11941 ttccgggctc atttcagcgc ggttaccgtt catggcattg aacagtaccc atgtttcgtc
    12001 atcatcgtca tccggttcgg gtgccataaa tgccccgccg ttgttcaggg tgtacagatt
    12061 ccagatacca ccgcagtagt cttcacacag acggtccatc cagccgaaga cacggggttc
    12121 cagggtgacc cactgtggaa tgaggccaaa atgctgcggc cagaagctga tgcgctgttc
    12181 atcagggacg ggagtggcaa ccagttgttg caggccagtc tccggtgcac aggccgaagc
    12241 ggtggtgttc tgagacaatg ttttcatgta tgaaatcctt gtaatgtgaa taaatgagag
    12301 ggagtaaagg tcgggagctg gcaacatcac gttcatcact gaccgtgatg acccggtaat
    12361 atcagggatg aatgatattc ggctgaaatt ccggacagcc gtacgcgtaa ccgttccggt
    12421 cacagacgga tggccagatg ccattgcctg tatactcagt ctgcacagac tgatttctcc
    12481 gggacggtaa agcagacggg tacaccaggc gcacaggtca tcatcagaaa tggaacgaca
    12541 ccccggcagc gcctccgggg cgtcgggtga ttctggtgcc tgaatcacgc cttcagggac
    12601 atgctgcctc agcgcaggct ctccagcagc gtttctgcca ttacccacaa tgcgcggttg
    12661 agcttaatat cggtgtcgat gttgtgaatg gcacgggtgt ggatacgttt tccttttgca
    12721 ctgcggccgg aaatcccgcc tttcagcata ttctcctgga tggtctgata tgcgctccac
    12781 aggtctttac cgtaatcctc cctgcgtcgt ggtgtcagaa tgtcagcggt ggtgactggc
    12841 tgatgttcgt caccataacg gtaagtcagt gccgcctgtg ccagcgcctg gcgtgccggt
    12901 ggcggcagga ccagcgactg catggcatca cgcttctcct ctatccggtc aaacacgccc
    12961 accacctcgt aagccccttc aataactttc tccaccacat ttccccggtg tggaacacgc
    13021 aattctccca gagactgacc gcagaccagt ccattggtac aaatggcacg aaaatatccg
    13081 ggtaacatct ggtagctgga tgaaccgtca tgagagttga gcagaataat ttcagggaca
    13141 tgctgaccgg ttatctgtcc ggcccgccgc agacgcagca tgtgttttgt gtattcccgg
    13201 cgacccgggt cacgcacacg tgtctggcag gcgaagaatg gctgaaagcc ttcccgctgc
    13261 aggctttcca gtacggtgat ggtgggagtg tacgcatagc gtttactgcg ggaagtatgc
    13321 cggtcttcac cgaaaatact gggaacatag tgcatcagtt cttcgtgtgt cagcggacgg
    13381 tcacggcgta tctggtttgc agcataacca aaacgactgg ctagtcgcat aatttgctcc
    13441 ttatcggtgg ttaagattta ctggtgtaat aaatgaaaaa gccacgtctc ccggagaaga
    13501 cgcggcctga tggacgaaat taatgaggtt tattgtctga gaagaccgtc actggcgaac
    13561 cttccggcgt cagtctctgc gttgcggata acccgacgga aatgagttcc ctggtcatcg
    13621 tcgataaaca cattgcggta agctgttgtg atgacaacag cctgctcgcg tgagaaaggg
    13681 acttcgggca ataaacgtga catacaacca gacctgtatt tggttccctg aaaatataag
    13741 tccctgtcat catggcagag ccgtatttac ttttgatatc aataaattaa taacgcgaag
    13801 atgaaccgca taacttacgc ctttaatttg ttttgtttac gtagtccctg accaggtacg
    13861 cgaattcgtg aacgccagtt tcaccaaacc aaatcaggta accagggtcg atgttgtaat
    13921 agtaaccaac gactttatgc gtttttgggt tatagagtgt tacgatatag tcccagccgc
    13981 aggcgtgggc tttacaaccg ccaccttcta cccaggtcgt tccgttaatg tttacaggat
    14041 acgcaccacc atcaggacca tccatatagc cgtcttcccc aaacattccg tttaacccaa
    14101 cagggtttaa tgctttgtcg atagcagctc tgtaggtagc gtcaccttcg tataactcgt
    14161 acgttgttgg acatgcacca ggctggtcag cccgggcttt tgagtcgcat ttaagtgtct
    14221 gatttacagt ggcggcgttt gtgtcaggac tt
//
LOCUS       DADMGC010000105        14000 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.105, whole genome shotgun sequence.
ACCESSION   DADMGC010000105 DADMGC010000000
VERSION     DADMGC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.105"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..170
                     /locus_tag="JNZ73_003053"
     CDS             <1..170
                     /locus_tag="JNZ73_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY8733258.1"
                     /translation="RINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPK
                     RYRKKLTEFNQ"
     gene            275..1627
                     /locus_tag="JNZ73_003054"
     CDS             275..1627
                     /locus_tag="JNZ73_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY8733259.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKNVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1640..2398
                     /gene="chbG"
                     /locus_tag="JNZ73_003055"
     CDS             1640..2398
                     /gene="chbG"
                     /locus_tag="JNZ73_003055"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HAY8733260.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2445..4706)
                     /gene="katE"
                     /locus_tag="JNZ73_003056"
     CDS             complement(2445..4706)
                     /gene="katE"
                     /locus_tag="JNZ73_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HAY8733261.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIREHVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4889..5152
                     /gene="cedA"
                     /locus_tag="JNZ73_003057"
     CDS             4889..5152
                     /gene="cedA"
                     /locus_tag="JNZ73_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HAY8733262.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5429..6245
                     /locus_tag="JNZ73_003058"
                     /pseudo
     CDS             5429..6245
                     /locus_tag="JNZ73_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597900.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6249..7639)
                     /locus_tag="JNZ73_003059"
                     /pseudo
     CDS             complement(6249..7639)
                     /locus_tag="JNZ73_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
     gene            complement(7772..8362)
                     /locus_tag="JNZ73_003060"
     CDS             complement(7772..8362)
                     /locus_tag="JNZ73_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY8733263.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(8525..9192)
                     /gene="hxpB"
                     /locus_tag="JNZ73_003061"
                     /pseudo
     CDS             complement(8525..9192)
                     /gene="hxpB"
                     /locus_tag="JNZ73_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
     gene            9339..9875
                     /locus_tag="JNZ73_003062"
     CDS             9339..9875
                     /locus_tag="JNZ73_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733264.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(9916..10776)
                     /locus_tag="JNZ73_003063"
     CDS             complement(9916..10776)
                     /locus_tag="JNZ73_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAY8733265.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPSFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(10882..11172)
                     /gene="ghoS"
                     /locus_tag="JNZ73_003064"
     CDS             complement(10882..11172)
                     /gene="ghoS"
                     /locus_tag="JNZ73_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY8733266.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(11273..12202)
                     /gene="pfkB"
                     /locus_tag="JNZ73_003065"
     CDS             complement(11273..12202)
                     /gene="pfkB"
                     /locus_tag="JNZ73_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HAY8733267.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVSRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            12488..13246
                     /locus_tag="JNZ73_003066"
     CDS             12488..13246
                     /locus_tag="JNZ73_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HAY8733268.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(13299..13406)
                     /locus_tag="JNZ73_003067"
     CDS             complement(13299..13406)
                     /locus_tag="JNZ73_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733269.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(13578..>14000)
                     /locus_tag="JNZ73_003068"
     CDS             complement(13578..>14000)
                     /locus_tag="JNZ73_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733270.1"
                     /translation="HDGEPGLYAAMENNHPLCVTRFLSKINGIAFKYKLSKANIMDLL
                     KGATAQGPPALYIAMSKGNEDVVLSYISTLGAFAKKHSFSQHQLFTLLAAKNHDNMSA
                     VHIAIHHKHYKTVETYYAAINVISQSLSFSADEIKTYL"
BASE COUNT         3611 a         3217 c         3457 g         3715 t
ORIGIN      
        1 tccgtattaa ttttgccaaa aaacaactgg aaatgaccaa ctattccgtg acggatattg
       61 cgtttgaggc cggttatagt agcccgagtt tgtttattaa aacgtttaag aaattaacgt
      121 cctttacgcc taagagatat cgtaagaaat tgactgaatt taatcagtaa gtcgttatac
      181 ctgacaattc acatatctgt cctgttgctg gattatttat gtccgggggg gcagatatgc
      241 cagatatcag tattctgtac tgaagggaga aattatgagc cagaaattaa aagtcgtcac
      301 tattggtggc gggagcagct ataccccgga gttactggaa ggatttatta agcgttatca
      361 cgaattgccg gtcagcgaat tatggctggt ggatgtcgag ggtggcaaag ctaaactgga
      421 tattattttc gatctctgcc aacggatgat tgataacgct ggcgtcccga tgaagcttta
      481 taaaacgctg gattgccgcg aagcattgaa agatgctgat ttcgttacta cccaactgcg
      541 cgttggccaa ttaccggcgc gtgaactgga tgaacgtatt ccattaagtc atggttatct
      601 tggtcaggaa accaacggcg cgggcggttt gtttaaaggt ctgcgtacca ttccggtgat
      661 ttttgacatc gtaaaagatg tcgaagaact ttgtccgaat gcatgggtga ttaacttcac
      721 taacccggcg ggaatggtta ctgaagccgt ttatcgtcat accggattta aacgctttat
      781 cggcgtgtgt aatattccga tcggcatgaa gatgtttatt cacgatgttc tgatgctgaa
      841 agacagcgat gatttatcta tcgatttgtt tggcctcaac catatggtgt tcattaagaa
      901 tgtgctggta aatggcaagt cgcgctttgc cgaattgctt gatggtgtgg catccgggca
      961 gttaaaagca tctggcgtta aaaatatttt cgatctgcca tttagcgaag gcttaattcg
     1021 ttctctgaat ctgttgccgt gttcttattt gctttattac ttcaagcaaa aagagatgct
     1081 ggctattgaa atgggcgaat actacaaagg cggcgcacga gcgcaggtag tacagaaagt
     1141 cgagaaacaa ctttttgagc tgtataaaaa cccggagttg aaagttaagc cgaaagaact
     1201 ggaacagcgc ggtggggctt attactctga tgcagcgtgc gaagtgatca acgctatcta
     1261 caacgacaag caagctgaac attacgttaa tatcccgcat catgggcata ttgataatat
     1321 tccggcagac tgggcggtag aaatgacctg taagctgggg cgcgatggcg cgacgccaca
     1381 tccgcgcatt acgcatttcg atgataaagt gatggggctg attcacacca ttaaaggctt
     1441 cgagattgct gccagcaacg ccgcacttag cggagaattt aacgatgtgt tactggcgct
     1501 aaaccttagt ccgttggtgc attccgatcg cgatgctgag ctgctggcac gcgagatgat
     1561 tctggcgcac gagaaatggc tgccaaattt tgccgactgc atcgcagagc ttaaaaaagc
     1621 acattaaccg aggctgatta tggaacgctt actgattgtt aatgccgatg attttggctt
     1681 aagcaaaggt cagaactacg gcattatcga ggcctgtcgc aatgggattg tcacatcgac
     1741 gacggcgctg gtgaatgggc aggctattga ccatgcggtg cagttgagtc gtgatgaacc
     1801 gagtctggcc atagggatgc actttgtcct tactatgggc aagccactga cagctatgcc
     1861 ggggttaacc cgcgatggtg tgctgggaaa atggatctgg cagttggcag aagaagatgc
     1921 tttaccgctg gaagaaatta ctcaggagct tgccagtcag tatttgcgtt tcattgagct
     1981 atttggacgc aaacctacgc atcttgatag ccatcatcat gtgcatatgt tcccgcagat
     2041 tttcccgatt gttgccaggt ttgcggctga agaggggatt gcgttgcgca tcgaccgtca
     2101 gccgctatct aacgctggtg atttaccggc taatctgcgc agttcgcagg gattcagtag
     2161 cgcgttttat ggtgaagaga ttagcgaggc actgttcctg caagtgctgg atgacgcaag
     2221 ccatcggggt gatccttcgc tggaggtgat gtgtcatccg gcgtttatcg ataatactat
     2281 ccgtcagagt gcttactgtt tcccacggtt aacggagctg gaagtgctga catcagcgtc
     2341 gttgaaatat gcaattgctg agcgtggtta tcggttgggt agttatcttg atgtgtaagt
     2401 aaggtggtaa ggtcactccc caggggacgt gaccttatcc atatttacgc cggaattttg
     2461 tcaatcttag gaatgcgtga ccacacgcgg tgttctgcca tcagcgttaa cagttcatcc
     2521 ataaaactac cgtcggtgct gtcagcttcc acaatccctt cttcaccctg gtcagctacc
     2581 ttgattgttg ctttaaactt gcgcgcgtct cccgccagcg caatcggttt aaggtgtttg
     2641 taggcttcca tcagatagta gttggcatcg ccgttgtcag cgatatccgc gatattgccg
     2701 caagggacaa tgaccgcatc gaccgtcagc gaaggtgcac cggcaaaggt agcggctata
     2761 ggcaacaccg taccgtcatc cgcagtcact tcacccattc gggagtagag cagtttggca
     2821 tgaacgcctt tggccttcag ggccttgaga atggcaagaa ggtctgccga tctcacttca
     2881 tcattaagta aaatcgctac cacgcgacct ttcacatcac cgtcaggaat ggcgtacaaa
     2941 cttaaggatg gatccttttt cagaccgttg acgtccggag gtggggtgat attcagctgg
     3001 tcgtcagtca gttcgatacc gagatttttc gccaccgcct gggccagagt gagatcaata
     3061 tgcgccagct ggtcaacaac gtgctcacga atatacggac gaacgacttt gcttaactca
     3121 aaactgaaac catcgacaat atggcgctgc tcgaatggcg tctgacttag ccagaacaga
     3181 cgcggatggg aataatattc gccaaacgat gggctgcgct cgcgaacttt attgccttcc
     3241 acgcgctctt ggtatgattc aaaaccgccg cgtttcggcc ccggtggtgt ttcgcgcggc
     3301 cagttatcgt tgatcgagtt cggttcataa ttcgccgggt tagtgtcgat ccccatacga
     3361 tgcatgccgt cacgctggaa attatggtaa gggcaggtcg ggcggttaat cggaatctca
     3421 tggaaattcg gcccaccaag acgactgatt tgtgtatcgg tataggagaa caaacgtccc
     3481 tgcaacaggg gatcgttggt gaagtccaga ccaggaacaa tatgacctgg atggaatgct
     3541 gcttgttcgt tttcggcaaa gaagttatcc ggattgcgat tgagcaccat tttgccgaca
     3601 cgctgaacgg gcaccagttc ttccgggatc agtttggttg gatcgagaag atcgaagtcg
     3661 aacttgaatt catcttcttc aggaatcaac tggaagccca gttcgtattc cggaaaatcg
     3721 cctgcttcaa tggcttccca caactcgcgg cggtggaagt ccgggtcacg tccggtgagt
     3781 ttttgtgctt catcccaaac gagtgaggct ttacctgcca gtggtttcca gtggaaacgt
     3841 acaaacgttg ccttcccttc ggcattaatc aggcggaagg tgtgaatacc gaagccttcc
     3901 atggtgcggt aactgcgggg gatgccgcga tccgacatcg cccacatcac gttgtgcaga
     3961 gtttcaggtt gcagagaaac ataatcccag aaagtatcgt gggcactttg cccttgtgga
     4021 attgcccagt gcggttctgg ttttaccgca tgaacaaaat cggggaattt atgcgcatcc
     4081 tggataaaga agattggcgt gttattgcca acgaggtcaa aaataccctc ttcagtatag
     4141 aacttggtgg caaaaccacg gatatcacgc acggtatcgg cagagccagc gccaccctga
     4201 acggtagaga acctaacaaa tactggggtg attttgttcg gatctgagag gaaatccgct
     4261 ttggtgatat cgcttaagct tttatatggc tggaaataac cgtgagctgc tgatccgcgt
     4321 gcatgaacaa tacgttccgg gatgcgctca tggtcaaagt gggtgatttt ctcgcgcaga
     4381 ataaaatctt ccagcagcgt tggaccacgg ttaccggcac gcagtgagtt ttgatcatcg
     4441 gcgatgcgca cgccctgatt agtggtcagc gcataatttt cactgccttt gcgtacgtct
     4501 tccagagaat taagtttttc gttacgtgta tcaggggctt tcaggctccc aggggcggta
     4561 ggttgtgcac ctggcggtgt tggttcagcc gctggacgat gagatccgtc ctcaggtgcc
     4621 agtgagtcca tccccggttt cgcttcgctg gaatcgtgta gtggtgactg gtgctgatgt
     4681 gggttctttt cgttatgttg cgacattgaa ctcgtctcct taatttatta ctgaaagggc
     4741 cgctgttttg gtcaaaaaac tctctaacta tagaccacca gccagattcc gcttcggaga
     4801 ctattctgga aacggtgtta tgaaatacac gcaaagcggg acgtacaggg gctaaatcgg
     4861 ctaaactaca gtttttctaa tttttaatat gcgtttagtg aagccagtaa tgaagaaacc
     4921 gctccgccag caaaaccgcc agattattag ctatgtccca cgcacggaac ccgcgccgcc
     4981 agaacatgcg ataaagatgg attcgtttcg tgatgtctgg atgctgcgtg gcaaatatgt
     5041 tgcgtttgta ctgatgggag agtcatttct gcgctcaccg gcgtttactg tgcctgaatc
     5101 tgcccaacgt tgggcaaatc agatccgcca ggaaggggaa gtgactgagt aaggattaca
     5161 tacctcctgg ttttgccggg agttacagga cctgaaattc tgcatttcgt tgctacattc
     5221 attcaaacca cctgtaacaa aaagctgcct ttgacgagct attgctataa ataagtgatc
     5281 ttttttcttt aattgtttat ttattttaaa atggttaagt tttgctaatc atgatgagtt
     5341 atttttgata gttcatcctg tggcgtcgct cttaaactta tattgtaaat ggcttagatt
     5401 taaataagat aataattttg ttaacaatat gaggcgggag atgttttcta caaacttaat
     5461 acagagtaat tatggtgatt taaatattaa aagcctggct ttcgactctt ttaaagaaag
     5521 gcttgagtct accatggcgg cactcacttt ctttatatca acgggtcaat gtgactgcga
     5581 tgaaatagca gagtcgaact tcaatcgtat gattgcctat atgtctaaca ttaactatga
     5641 tgcctcaaag ctaggagcgc cagcacttag ttttgatacg tatctgcagg acaatgttaa
     5701 atatagagtg ataattaata atctttatgg cagtgagttt cggataagag atattaatga
     5761 ggattttgtt gggatggatt ttacctgtga attccgaccc gaaaaaatga cgaatttgat
     5821 caatataaaa aataggttgg ttattcatta tttaaggact atatactatg aaaaaaatta
     5881 tatacatcct gccgggagca tttttgctgc gattcaaaaa aaatgagagt ctcctaaagt
     5941 ttccttcgat aatatcaatg cttaatatca atcttctttt taatccacta aatcttccgg
     6001 gaatgggaag tggtgtgctt gaagatatta tgagtattcc agatagttca cttagaaaac
     6061 gtttcgggta tgaggtttta agcttctcac tacaggcaca ttcattgagc caggaatgta
     6121 ttgataagtt ggacattttt ttcgctgacg atctgtttaa atatgagtca gtatgcatcg
     6181 ccgcgatgga acatttaaaa agtaaagcaa cggcccctat acaaaacgga ccgttgccag
     6241 cataagaatt aacggtgtgc cagttcggcg tcgtcttcgc tatccagaat ggctttatcg
     6301 gtttgcttca gccactggct ggtcagcgtg ccagctgtca tcgagccact aacgtttagc
     6361 gccgtacggc ccatgtcgat aagcggttca acggagatta acagcgccac cagggttact
     6421 ggcaggccca tcgcaggcag cacaatcagt gcggcgaaag ttgcaccacc accgacaccg
     6481 gcaacgcctg cggaactaac ggtaacaata ccgaccagcg tcgcaatcca catcgggtcc
     6541 agcgggttaa tgccaaccgt aggcgcaacc atcaccgcca gcattgccgg atacaaaccg
     6601 gcgcagccgt tctgaccaat ggttgcaccg aaagaggcgg cgaaactggc gatggattca
     6661 ggaacgccca gacgacgcgt ttgtgcttcc acattcagtg ggatagacgc agcactggaa
     6721 cggctggtaa aggcaaacgt cagcacaggc cataccttac ggaagtactt cagcggactc
     6781 acgccattaa tgcccagcag aatgccatgc acagcaaaca taatcagcag accgaggtag
     6841 gacgcgacaa cgaaacttcc cagtttgatg atgtcttgca agttagaacc tgcaaccact
     6901 ttggtcatta gagccagaac gccgtaaggg gtcaactgca tgacaaggcg aaccagtttc
     6961 atcacccagc tttgtagggt atcgatagcg gttaagacgc gttcaccttt cggcgcatca
     7021 tccttcagca gtttcaatgc agctacgccg aggaatgcgg caaaaattac cacgctgata
     7081 attgacgtcg gattggctcc ggtaagatcg gcaaacgggt ttttcgggat aaaggacaag
     7141 accagctgcg gaacgctcag atcagagact ttaccaacat agttactttc aatggcgttc
     7201 agacgtgcag tttctgcacc accctgaacc agaccttcag ccgtcaaacc aaacaggtgg
     7261 tgaccagcac accgaccagc gccgcaatca gcgtggtaaa caaaagcgta ccgatggtca
     7321 gaaaactgat tttgcctaac tgagatgcgt tatgcagacg ggcaaccgcg ctcagaatag
     7381 aggcgaagac taacggcata acgatcattt gcagcagttg aacattgccg ttaccaacga
     7441 tgttaaacca ctgtacagaa tctttaagta cctggctgtc agaaccataa atggtatgca
     7501 gggcaaggcc aaaaaccaca cccatcacca gacccaccag cacttttttc gccagactcc
     7561 actgtttatg gcgggtctga gccagcgcaa acagcagtac aacgaacacc acgatgttcg
     7621 caattaatgg aaagttcatc cccgttctcc tgatcttatt atttctatca ctgcgtagca
     7681 gtcatagtgt tggcgcaaga ttaacagaac tgtgagctgg ctcttatatt caaatggaat
     7741 tgcttattcc agaaaggtga tttttgcctt tttagtattc aacctgatgc ttaataagcc
     7801 gatgaaactg gatctgcaag gtattgatca tttgcgatga ccaacgaaca gcgctgttct
     7861 cgggtaatga atggggcatc cataccgacc agacaaataa tgccatgcag ataaaacgtt
     7921 ccagttggtt gcccttttgg ggaaccagga taggcaagcg gaaacgccag cggcatggcc
     7981 agagcagggg aacaccggcg ggtgtcagca tatcggcaag tatgtggctc aaataaccca
     8041 gcaccattcc ttgtagcgca tcagccggaa tgaaccagcc ttccggaacc ttaaggtaaa
     8101 aggttgccag cagcgcaaat accgccagca gactgtgggt aaaaccacga tgcccaaaag
     8161 cgcgggcgat cggttttgat atccatttta atcgctgccc aagaaacgac tttgggtgat
     8221 cgatgtccgg taacaaacac gtcaggattg cggaagggac aatatgccac cagtcaccct
     8281 gtgccagcac gggcgtcagc tcggcatttt tggcaaatac cgcacaagca atagaaaaga
     8341 gaaggtgacc ttccgccgtc atgataaaac tcactaaact gtcgatacgt acagtatagg
     8401 atttttatac agtgcacgga agtggtgacg gtgtaactct ttctcttaaa ttgacgatta
     8461 atccgtcagt tgaatgcaaa ttcagataaa gaaccagggg cagcgtgatc actgcccctg
     8521 gtcattaacc gagaaggtct tttgcggtga gttctgtcag cgatgagagt ttgacgtttg
     8581 ctaatacaaa acgtggatca ttttgcgctt ctggcgcagg aacgacgatg gaacgcatgc
     8641 aggctgcttt agaagcgatc atgccattta ccgagtcttc cagcgccacg caggtcagtg
     8701 ggtcaacgcc cagttttgct gcgcagtcga gatatacttg cggatgcggc ttgctgtaag
     8761 gcagtttttc agccgaggcg agggcatcga aactgtcgcg taagtcaaac atggtcaaca
     8821 ctttttccag catatgtagt ggtgacgcgg aggccagtcc caccaataaa ccttgttctt
     8881 tgcataacgc aacagcttcg cgcacgcctg gtaataatgg acgtgtctct tcaaccagtg
     8941 aaatggcacg ggcaataacc cgttctacta cttcctgacg gcttggccca ttccacggtt
     9001 gccgggcgta caaagatcga ccaccatatc gatgcgtaaa cctaaggtgt ccggcagctc
     9061 gttacgacgg gagatatcta cccccaggct tgccatcaca tccagttcgg ctcgatccca
     9121 taaaggttct gagtcgataa gtaatccatc catatcaaaa attgcagcaa gaatctgacg
     9181 cggggttgac attacaacct ctcctttaga gcattatgtg taaggtatta agccgtgctt
     9241 atgattaagc gcctatcata tcgcctttaa agaatgggcg aaaacagata tctacaggta
     9301 aactaagtga aacgacgcgt cttaatcaag gagaactcat gacgtatcaa caagctggac
     9361 gcattgctgt tttgaaaagg attttgggtt gggtgatttt tatacctgca ttgatctcta
     9421 cattgatttc tttgctgaaa tttatgaata cccggcagga aaaccaggaa ggcattaatg
     9481 cggtcatgct cgactttgct catgtcatga tcgatatgat gcaggcgaat acgccttttt
     9541 tgaatctgtt ctggtataac tccccgaccc ctaattttaa tggtggtgtg aacgttatgt
     9601 tctgggtgat tttcatcctg atttttgtcg gactggcgtt gcaggattcc ggtgcccgga
     9661 tgagtcgcca ggcgcgcttc ttgcgggaag gtgttgaaga tcaactcatt ctggaaaaag
     9721 ccaaagggga agaggggcta acgcgtgaac aaattgagtc ccgtattgtt gttccgcacc
     9781 atactatttt cctgcagttt ttctcactat atatcctgcc ggttatttgt attgctgcag
     9841 gttatgtgtt cttttctctg cttgggttta tttaatcaaa aggccgcacg gagcggccat
     9901 tatcctcaac ccatatcatg ctgctaataa tctatccaat gactgctgag caataaccaa
     9961 atgctgaccg ccaaataatc ttgcacgatt taacagcgtg tagagttggt aaaccggttg
    10021 acgttcaagg aaatcggcag gtagcgggga tactgactga tagccgtcat agatttgtgg
    10081 cggctgttca gtatgcagcg gtaacatcgc caggtcgcac tctcggtcac cccagtagca
    10141 ggcagggtcg aaaatgtacg ggccatccgg acccagcgca cagttgccgg accataaatc
    10201 gccgtgcaac agagaaggct gcggttgatg cgaggccaga cgctgctgaa tatgctcgac
    10261 gatggcgtcg atattgccga aagcgatccc tttctctgct gccagttcca actgccagcc
    10321 aatccgttgt tcagcaaaaa acgttgacca gcgacgttgc caggtgttgg gctgtggagt
    10381 tgtggagagc gagttatcaa aatcgaggcc aaattgtggt tggtcactcc attgatgtaa
    10441 acgcgcaatt tgctgaccaa gaataaatgc gctatgcgca tccagcggac gaggtgggag
    10501 ataatccatc accagaaaac tgtagtcacg gtcagcgcct actgcccaaa ccttaggcac
    10561 ggtgacggtt ttactacgcg acagtaactc cagttggtcg gcttcggcgg taaaactggg
    10621 aagcagttcc ctttcatcac atttgacgaa aaagtcatgt cctgcatagc gcaaatgcca
    10681 tgcggcatgg acttctccgc caggcagttc attacgcagt tcgatttcgc cttcacctaa
    10741 ctgctcgctc aaaagacgac tgattgcctg ccacatgatg tctctcccat gttgtctgcc
    10801 agatcataag gttagcgcaa gaatgcggtg taaaaacacg agctaacgca caccacagac
    10861 tgattattgc ccaaaaacca tttattttct gttgctattc cattcctccc aggtcgtgat
    10921 ggtgatttca ggatctttgc cggttgcact ttgggtaagc cccgaaacca gttctctaat
    10981 ttcttcttca ctttgggtac tgataagtcc aaaagtgtta gtccccagct catggatatt
    11041 tccctcatca tccgtcatgg tgagcaaaaa accgtcgcga gtcaggtaat tattcaactc
    11101 gttaatttca gtcaatgtat cctcatgcaa catgacggtt atgacataac ggacaagatc
    11161 gccactggcc atagttcacc cctttgttat cacatgactt ttgagcatag tcggagaaac
    11221 gcgttgccga caggttggtg gtgattcccc caatgctggg ggaatgtttt tgttagcggg
    11281 aaaggtaagc gtaaattttt tgcgtatcgt catgggagca cagacgtgtt ccctgattga
    11341 gtgtggctgc actccccgca gctacgccaa aacggaccat ctcttcaaga gaggcatttt
    11401 ctgccagttt cagtgtcatc gcgccgacca tgctgtcacc agcgccaacg gtactctggc
    11461 ttttcaccgg tggtggcacc acctgaatgc agttttcact atcaacaccc agcgctcctt
    11521 gtggaccaag ggaaacgaca acccgtttgg ccttgccgct attaacgatt tcctgcgcgg
    11581 ctttgcggac atcgtctggc tgggtgagtt cgcgactcac cagcgcactg agttcttttt
    11641 ggttaggctt aaccaactcg atgttaccaa tcgccagtgc tgcacttaac gcttcgccag
    11701 aactgtcgat gatgcagcgg atcccttggt tttgcgcagc ggaaatcagt tgggttaatt
    11761 tttccagctt cacacctggc ggcaggcttc cgcttatgac caggatggcc ccggattcaa
    11821 tttccagaac ttgctcttca agctggcgaa actcatcttc atttaatgcc gcgcctggca
    11881 taacaaaacg atactgttca ccgcttgctt ccacatgtac gtgtaaattc tgccgggtcc
    11941 agtctttggc ttctacagta gcgacgggga cattttcatc cgccaacagt gaaaccaggt
    12001 gttcgccggt cgcgccaccc gccgggaaga tcgctgtggc actgcctcca agatgggcaa
    12061 tggcgcgggc gacgttgatg ccgccgcccc cgggttcgaa caccggtgcg gtacagcgca
    12121 gttttccttc gggataaatt tgcggggtaa ttgttgcgct atcgagagag ggcgcaagtg
    12181 tcaacgtata gatacgtacc atcatttcct cctataggct gatttcagtc tggcaccgaa
    12241 tcagcggaaa gtgaagaaat taacaatatg atttattaga gctttattta aaattttgca
    12301 gataaatata tataaataaa aatctgtttt cgccacatgc ttattcaaaa aatgaaataa
    12361 gaatacattg ctaagttatt cgcgcctttt tataattcgt tacctttcca gggacgctaa
    12421 tctttgatcc ttaagaaagt ttccgggacc gtgatggtct cttttttgtt gtacggactc
    12481 ttgataaatg aagcttttga agacagttcc tgccatcgtt atgttggcgg ggggcatgtt
    12541 tgcgtcactg aatgcagctg ccgatgattc cgtttttact gtcatggacg atcctgccag
    12601 cgctaaaaaa cctttcgaag gtaatctgaa cgcaggatat cttgcacaat caggcaatac
    12661 gaaaagctcc tcacttacgg ctgacaccac catgacctgg tatggacaaa ccactgcctg
    12721 gtcgctgtgg gggaacgcca gtaatacttc ttctaatgat gagcgttctt cagaaaaata
    12781 tgcggcgggc gggcgtagcc gctttaattt aactgattat gactatttat ttggtcaggc
    12841 aagctggctg acagaccgtt ataacggcta tcgagagcgc gatgtgttga ccgcgggtta
    12901 tggtcgtcag tttttgaatg gcccggtaca cagtttccgc tttgaattcg gtccaggtgt
    12961 gcgttacgac aaatataccg acaatgccag tgaaacccag ccgctgggct atgcctccgg
    13021 ggcttatgca tggcagttga ctgacaacgc taaatttacg cagggtgttt cggtctttgg
    13081 cgcggaagac acaacgctga actccgaaag cgcattaaat gttgctatta atgaacattt
    13141 tggactgaag gtggcttaca acgtcacatg gaactctgaa cctccagagt ctgcaccgga
    13201 acataccgac cgtcggacaa ccttatcgct gggctacagc atgtaaaatt atctgccgga
    13261 ccgtgttatg cggtctggct gtcttcgttt gtcactgctc agccccaaat cattgctgcc
    13321 caacgtagca acaacatagc gccacagatt gccagtagca ccaccaccat cttccagtgt
    13381 tttttatcaa agcgtttagt cggcatacca gtgtcctgca tatctcttgt gcgtgataac
    13441 ggagtgtaac aaaagggaca ggcatcaagg gattttttgt tattacagcc gtcgggtggc
    13501 gttgtgcaca tggtcactgt ttctgcgttt tatgaaaaaa ataaaattat gtatcgatat
    13561 atatcgtaaa tagctggtta taaatacgtc tttatttcat cagcactaaa actcaggctt
    13621 tggctgatga catttatagc agcataatat gtttctacag ttttataatg cttatgatga
    13681 atggctatat gaacagctga catgttgtca tgatttttag cggccaatag tgtaaataac
    13741 tgatgttgac taaaagaatg tttttttgca aaagcaccca gcgtcgatat ataagataac
    13801 acgacgtctt cattaccctt gctcatggcg atgtataatg cagggggtcc ctgtgctgta
    13861 gctcctttta ataaatccat gatgttagct ttgctcaact tgtatttaaa ggcgatgcca
    13921 ttaattttag agaggaaccg tgtgacacac aaagggtgat tattttccat tgcggcgtat
    13981 aatcctggct caccatcatg
//
LOCUS       DADMGC010000106        13956 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.106, whole genome shotgun sequence.
ACCESSION   DADMGC010000106 DADMGC010000000
VERSION     DADMGC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.106"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            296..1549
                     /gene="kch"
                     /locus_tag="JNZ73_003069"
     CDS             296..1549
                     /gene="kch"
                     /locus_tag="JNZ73_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HAY8733271.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1604..1777)
                     /locus_tag="JNZ73_003070"
     CDS             complement(1604..1777)
                     /locus_tag="JNZ73_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HAY8733272.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1920..3380
                     /gene="cls"
                     /locus_tag="JNZ73_003071"
     CDS             1920..3380
                     /gene="cls"
                     /locus_tag="JNZ73_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAY8733273.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3415..3744
                     /locus_tag="JNZ73_003072"
     CDS             3415..3744
                     /locus_tag="JNZ73_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HAY8733274.1"
                     /translation="MDMDLNNRLTEDETLKQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="JNZ73_003073"
     CDS             complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="JNZ73_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HAY8733275.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="JNZ73_003074"
     CDS             complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="JNZ73_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HAY8733276.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGAATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="JNZ73_003075"
     CDS             complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="JNZ73_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HAY8733277.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="JNZ73_003076"
     CDS             complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="JNZ73_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HAY8733278.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLEHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="JNZ73_003077"
     CDS             complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="JNZ73_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HAY8733279.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="JNZ73_003078"
     CDS             complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="JNZ73_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HAY8733280.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11245..13920
                     /gene="adhE"
                     /locus_tag="JNZ73_003079"
                     /gene_synonym="adhC"
     CDS             11245..13920
                     /gene="adhE"
                     /locus_tag="JNZ73_003079"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAY8733281.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3657 a         3455 c         3247 g         3597 t
ORIGIN      
        1 agatccaata tttaagggca cggtttttgc aaccgtgttc ttgtttggcc tttccttata
       61 gccggtcagt gcattcacta ccacaagcag ataataactt tatctgtatt tctttatatg
      121 atgtgagcat gttcaggaaa aaagcccttt cctgacgcag agttgctcac tcaaaacctt
      181 tgattactgc ggataaagtc atctactatt gtttaacaca ttcacgcgct gcatgtatgt
      241 tcagaaaatg tgcacgctaa gataattaaa caaaattgtg gttacagagg tatcagtgag
      301 tcactgggct acattcaaac aaacagcaac aaatttatgg gtgaccctac ggcacgacat
      361 tctcgcgctg gccgtctttt taaatggatt gcttattttt aaaacaatct atggtatgtc
      421 ggtcaatttg cttgatattt tccatatcaa agcattttca gagttggatc tctccttgct
      481 ggcaaacgcc ccactattta tgctcggcgt ctttcttgtc ctgaactcca ttggcttact
      541 gttccgggca aagctcgcat gggcaatcag tatcattttg ttgttgatag cgctaattta
      601 caccctgcat ttttatccct ggctgaaatt tagtattgga ttttgcattt ttacgctggt
      661 gtttttgctg atactgcgca aagacttctc ccacagtagc gccgcagccg ggacaatttt
      721 tgcatttatt agtttcacga cattactgtt ttactcaacc tacggtgcac tttatttaag
      781 cgaaggtttt aatccgcgaa tagaaagttt gatgaccgcg ttctattttt cgatagaaac
      841 catgtcaacc gtcggctatg gagatattgt ccctgtttct gaatcagcac gattgttcac
      901 tatttcggtc attatttccg gcattaccgt ttttgccaca tctatgacct caatttttgg
      961 tccgcttatc cgcgggggat tcaacaaact tttaaaagga aacaatcata caatgcatcg
     1021 taaagatcat tttattgttt gcggacattc gattctcgcc atcaatacga tcctgcaact
     1081 gaatcaacgc ggacaaaacg taacggttat cagcaacttg cctgaagatg atatcaagca
     1141 acttgagcaa cgcttaggcg ataacgctga tgttatcccc ggtgacagta atgacagttc
     1201 agtattaaag aaagcgggaa tcgatcgttg ccgggccatt ctggcgctga gtgataacga
     1261 tgcagataac gcgtttgttg tactctcggc aaaagatatg agcagtgatg tcaaaacagt
     1321 tctcgccgtc agtgatagca aaaacctgaa taagattaag atggtacatc cggatatcat
     1381 tctctcaccg caactgtttg gcagcgaaat tctggcgcga gttttaaatg gtgaagagat
     1441 taataatgat atgctcgttt caatgttgtt gaactcaggt catggtattt tcagcgataa
     1501 cgatgaacaa gaaacgaaag ctgacagtaa agaatcggcg caaaaataga gtttaacgac
     1561 ggaagagtaa acttattggg caccgcaatg gtgcccagta catttagata ttgtagatcg
     1621 caaacaataa cgagttacgc tgtttgttta gaatgcactt cctgatgctg tggatacgca
     1681 tatttcggcg cgattgcccc tcaagccaac gcgatttacg tcggctagcc tgacgctgca
     1741 tgcgccagcg ccccacttcc gttctactcc gcttcatgtt aactactcta tgcaataaca
     1801 gaatggtcat tatacccccg cccccgacgc tgaccagtgc ttttcccgcg tttttatttg
     1861 ccagatgaat ccatatgcgt aaactcataa caatgcgctt tcaaaaggat ttctaatcta
     1921 tgacaaccgt ttatacgttg gtgagttggt tggccattct gggatactgg ttgctcattg
     1981 caggcgtaac tttacgcatt ctaatgaaac gacgcgcagt tccctccgcg atggcctggc
     2041 tgttgattat ttacattctg ccgttagtcg gaattattgc ctatcttgcc gttggcgagc
     2101 tccatttagg caaacgccgc gctgagcgcg ccagagcgat gtggccttcc accgcaaaat
     2161 ggcttaacga ccttaaagcc tgtaagcata tcttcgccga agaaaatagc agtgtcgctg
     2221 cgccattatt caagctttgc gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc
     2281 tacaactgat gaccgagtca gatgatgtga tgcaggcgtt aatccgcgac atccagctcg
     2341 cgcgccataa tattgagatg gtgttttata tctggcagcc cggcggcatg gcggaccagg
     2401 tggctgaatc attaatggcg gctgcacggc gtggtattca ttgccgattg atgctcgact
     2461 ccgccgggag tgtggctttt ttccgcagcc cgtggcccga gctaatgcgt aatgccggta
     2521 ttgaagtggt cgaagcctta aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc
     2581 tgcgccaaca tcgcaagatg atcatgatcg ataattacat cgcgtacacc ggcagcatga
     2641 atatggtcga tcctcgctac ttcaaacaag atgcgggcgt agggcaatgg attgatctga
     2701 tggcgcgtat ggaaggcccc atcgccaccg cgatggggat tatttattcc tgcgactggg
     2761 agattgaaac cggaaaacgt attctgccgc caccaccaga tgtcaatatt atgccgtttg
     2821 aacaggccag tggtcacacc attcacacaa ttgcttctgg ccccggcttt ccggaagatc
     2881 tcattcacca ggcattattg actgcggctt attcggcgca tgaatatttg atcatgacca
     2941 cgccctactt tgtgccaagc gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg
     3001 gggtggatgt cagtattatc cttccgcgaa aaaatgactc gatgctggtc ggctgggcca
     3061 gtcgcgcatt ctttacggaa ctgcttgctg ctggggttaa aatttatcag tttgaaggcg
     3121 ggttactgca taccaagagc gtgctggtcg atggcgaact aagtctggtt ggcaccgtta
     3181 accttgatat gcgtagtctg tggctgaatt tcgagattac cctggcaatc gacgataaag
     3241 gttttggtgc agaccttgcc gccgttcagg acgattatat ttcgcgttca cgtctgctcg
     3301 atgcccgttt atggctaaaa cgtccattat ggcaacgtgt cgccgagcga ctgttttact
     3361 tcttcagccc gttgctgtaa aacgtcgcca acagaggtta aacaggtagt gactatggat
     3421 atggatctaa acaatcgcct gactgaagat gaaacgctta agcaggccta cgatattttt
     3481 ctcgaactgg ctgccgacaa tctcgatcca gctgatgttc tgctgttcaa tcttcagttt
     3541 gaagagcgcg gcggcgcgga gttattcgat ccggcggaag actggcagga acatgttgat
     3601 ttcgatttga acccggactt ttttgccgaa gtggtgattg gtctggcgga cagtgaagat
     3661 ggtgaaatca acgatgtttt cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt
     3721 tgccatatca tctggcggga ataaataatc gcttttagcg tgaaaaaggg ctgacaactg
     3781 tcagccctta ttgtttttaa agcggatcga ctttcaggca agaaacggcg tgtctgaaac
     3841 tcccctccag aacaggacgt gttttggcgc actccggacc ggcaatcggg caacgggtac
     3901 ggaaaacaca accggaaggc gggttgatcg gcgacggtaa ttccccttcc agtaactgga
     3961 tggttttgtt cttctccaga tccggatcag gtatggggac tgccgacatc aatgccctgg
     4021 tgtaaggatg tagtggattg tggtagacct catcataggt ccccagttct actgcatggc
     4081 cgagatacat caccaacaca cgatcggaaa tgtgttttac cacggccagg tcatgagcaa
     4141 taaaaattaa tgacaatccc atctcacgtt gcagctgctg gagcaggttg accacctgcg
     4201 cctgaattga cacgtccagc gccgaaaccg gctcatcgca gataatcagc ttcggttcaa
     4261 gaataagagc acgagcaatc ccgatacgct ggcactgccc accggagaac tcatgcggat
     4321 agcggttaat caggttaggc aataacccga ctttcagcat catcgccttc acgcgctcgc
     4381 gaacttcctg gcgtgacatt ttcggatgat aagtacgcag tggttcagcg atgatctcgc
     4441 cgatggtcat acgcgggttt agcgatgcca acggatcctg gaaaatcatc tgaatatcac
     4501 tgcgaacggc acgccattca tcgggcttca tgcccagcaa ctctttacct aaccaggcaa
     4561 catgaccgtc ggtcgccttg accaaaccga tgatggcgcg agcaaaggtg gacttaccgc
     4621 atcccgattc ccctaccaca cctaatgttt ccccttcata caggcgaaga gtgacaccat
     4681 cgacggcttt gagcgttttc ggcggttgcc agaaccactg tttgccatct ttgatttcaa
     4741 agtgcacttt aagatcggcg atttcaagga ggacttttct tccttcagtt acagcattca
     4801 taacagttcc tccaccggtt taaagcaagc acgcagacgg ccaggcgtaa actcttccag
     4861 cggcggtgcg ctactacaaa tttccatcgc atgtggacaa cgtggctgga acgggcaacc
     4921 tttcggtaat cgcagcaagt ttggcggatt acccgggatg gtcaacattg tttcaccttc
     4981 cgcatcaaga cgcggcaccg cgttgagcaa accgatagaa taaggatgaa cgggttgata
     5041 aaagacatcg cgcgcattgc catattccat cgtgcgcccg gcgtacatta ccagcacttt
     5101 gtcacagatc cccgccacca cgccaagatc gtgggtgatc ataatgatgg cagtattaaa
     5161 ttcccgcttc agttcattca atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc
     5221 tgtagtgggt tcatccgcaa tcagcaactt aggtcgacat agcaatgcca tcgcaatcat
     5281 gactcgctga cgcatgccgc cagaaaattc gtgcgggtac attttcatgc gtttacgtgc
     5341 ttccggcatt tttaccgcat cgagcatccg caccgactct tcaaacgcct cagctttgct
     5401 catgttctta tgcagcatca gcacttccat caactgctca ccgacgcgca tatagggatt
     5461 caacgaagtc attgggtcct gaaaaatcat tgagatttgt tcagcgcgca gtttattgag
     5521 ttcacgctct ggcaaattga ggatttcacg cccattgaag gttgccgctc cgccaatgcg
     5581 tccgttggca gccaacaggc ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga
     5641 ctcacctaca atgcccagcg tttctccggc acgtagggaa aaatttaaat cattgaccgc
     5701 cgtgacgtcg ccgtccgggg tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc
     5761 gtcagcctgt tgttgtgcga gcggcacagt tgcagtttca attacgctca tggctgcact
     5821 ccttaacgat ctttcgggtc gagggcatca cgcaagccat cgccgataaa gttgaaacaa
     5881 aacagtgtca ccacaaggaa tcccgctggg aacaacagta accatggaga gacttccatc
     5941 gagttcgcgc catcactcag caatgccccc cagctgctta acggctcttg cgtacccaac
     6001 cccaggaagc taaggaaaga ttcaaagagg atcatgctgg gcaccagtag cgatgcgtag
     6061 accaccacca caccgagtac gttcggcaca atgtggcgaa taacaatgcc cggcgtcgat
     6121 acaccgccaa cttgtgccgc ctcaataaac tctttgcgct tcagactcag ggtttgccca
     6181 cgcacaatac gagccatatc cagccaggaa accatgccaa tcgccacgaa aatcagcagg
     6241 atgttttggc cgaaaaaggt caccagcaaa atgacgaaga acatgaatgg gaaggagttg
     6301 aggatttcca gcagacgcat cattaccgaa tccactttac cgcccagata accggaaagc
     6361 gaaccataaa gtgtccccac gaccactgcc accagtgccg cagcaacacc gaccatgagt
     6421 gagatacgcc cgccaatcgc aacgcgcaca agcaggtcgc gaccggatga gtcagtacca
     6481 aagtagtgac cggactccat atccggggcg ctggacatca tcgcccagtc agtatcgtca
     6541 taggcaaact gggaaagcat cggtgccagg attacaaata acgcgatcag caccagcact
     6601 atcagactgg caaccgccgc acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag
     6661 ctgcgccctt cgacctccag cttttcactg aaattttcca gcgtctcgct gtttttctta
     6721 cttaacatca tcgcgagctc cagtatcagt aacggatttt cgggtcgata accgcatata
     6781 gcacatcgac aatggcatta aacaaaatgg ttaacgcacc aaccaggatg gtcaggctta
     6841 acactaagga atagtcacgg ttcaatgcgc cattaacgaa cagttgccca atccccggca
     6901 aaccataaat ggtttcgatg accatagaac cggtaataat gccgacaaat gcggggccca
     6961 tataggagag cacgggtaac agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca
     7021 taggtaaccc tttcgcccgg gcagtacgaa taaagttgga gtgtaatact tcaatcatag
     7081 agccacgggt aatacgcgca atactggcga tataggcgag tgacaacgcc accatcggca
     7141 atatcatgaa tttaagcgcc ccaccattcc agccaccgcc cggcagccaa tgcaaaatga
     7201 tcgcaaatat catgactaat aatggcgcaa ccacaaaact ggggataaca accccggtca
     7261 ttgccagccc catcacggta tagtcccatt tggtgttttg ttttaatgcg gcaataacgc
     7321 cagcactaac acccagtatt accgcaagga aaaatgcggc ggctcccagt ttggcagaaa
     7381 cgggaaaact ggatgccacc aggtcattga ccgaataatc tttatattta aacgatggac
     7441 cgaaatcgcc atgctccagt tgtttcaggt agctgaaata ctgtgtcatg atcggatcat
     7501 taagatgata tttcgcttcg atattggcca tcacttctgg cggtaaagta cgttcgccgg
     7561 taaaagggct tcccggcgcg aggcgcatca taaagaacga aatagtaata agaataaata
     7621 gcgtcggaat cgcttccaga cagcgacgta gaataaattt taacattgcc ctaccttctg
     7681 gcctgtgcct tctgtaatgc gataaaaatc agacaccgtg gagcaggaca ctcctgcccc
     7741 acgtattgcc attagtgctt cacaatgtac atattccggg tataggtatt atccagcgga
     7801 tctttgccgg tatagccacc aacccacggt ttcaccagac gcgcattcac gtagtaataa
     7861 acaggaacaa tggccgaatc cttatccagc tgttgttctg ctttagtgta cagagctgtg
     7921 cgctgcgcct cgtcagtcac tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc
     7981 ttataatgcg cggtattcat cgagctgttc gacagcatgg tgttcaggaa ggaagttggt
     8041 tcgttgtagt ctgcacacca gcctgcacgg gccacatcaa aagtgccctg gtgacgggtg
     8101 tcgaggaacg ttttccactc ctggttaacc agtttgacgt ttacaccaat gtttttcttc
     8161 cacaatgaag aggcagcaat cgccagcttt ttatgcagat cggaggtgtt ataccgcagg
     8221 ttgatggtca acggtttgtc tgcggtataa cccgcttcag ccagcagttt tttcgcttct
     8281 tcgttacgtt tttcctggct ccagccaaac cattctggct gagtcaattt tgcgccatca
     8341 gtatacggtg gagtgtaacc ataggcgggc atgtcgccct gcgctttcac tttattaaca
     8401 atgatatcgc ggtccatacc tagtttcagt gcggtacgca cacgcacatc gttgaatggc
     8461 ggtttctggt tgttaatttc gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg
     8521 tccgggatct cttttttcag cttctggaac aattcgatcg gcatgttgtt atacgtcatg
     8581 tcgatttcac cactacggta gcggttgaca tcggtaactt cagaagcaat aggcaaatag
     8641 gttacctggt taataacggt tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg
     8701 attcgttcgt ttacgaccca atcttttaag gtataggcac cgttggtgac gatattacca
     8761 ggctgggtcc atttttcgcc gaatttctcg atagcggctt ttggcaccgg tgaagttgat
     8821 gggtgaacaa gtaatttata gaagtacgga acgggttcac ttaaggtgac ttctaatgtg
     8881 tgatcatcaa tagctttcac gccgagatcg gtaatcggtt ttttcccttc aagaatttca
     8941 tcaataccgg cgatatgccc atattgcaga taactggcat acggagaagc agtgttcgga
     9001 tcaacagaac gttgccagct atacacaaag tcttgtgctg tgactggcgt gccatcagac
     9061 catttcgcat ctttacgcaa atggaaggtc cagactttcg cgtctttatt atcccaggat
     9121 tcagcgacgc caggtgctgg atgaccgtca agatcgctga ccagtaagcc ttcaaacagg
     9181 tctcggctga tattagactc cggaacacct tcaattttgt gcggatctaa tgactgaact
     9241 tctgaaccat tgttacgtac cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca
     9301 tcagctgcca gcgcgacatt ccctgccatt agcgcagcca gaacgccagc tgctactaaa
     9361 cttctcttgg tgatgttggt cattgttttt tggactccct cattataatt actggtatta
     9421 accagcatgt gtaatcccgg attgggaccc tgcacaggct cggagacttt ctgctgtcag
     9481 gttactttat cgttacgtat tactatcacc gactttattt attctggtga ttcagacgat
     9541 caccttatgt cgattcgtac tgtgtctccc ctgtcgagac acattattct ttcgcgacgc
     9601 tctttaattg ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag
     9661 taccaaatgc ggttcttact cgccaataca ttttgcaaaa ttgttaagcg attctctttt
     9721 attgaccgta tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa
     9781 tcaaatagag attggctaag catgtctatc ttttctgatt acagttccag tagtgaaatg
     9841 cacaataatt taacaattga ttattattta gcactttcct ctaccaaagg tagtggaatt
     9901 actaatatca tttcaattat tcttcagcaa gcccaggatt atgatgtggc gaaaataaca
     9961 tgatggtgag gagaagcagt taaggaaatc cttataacta actgtaattt gagaagaata
    10021 tttttaccta ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa ataatgacga
    10081 agtaaacgga gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga
    10141 aaatcccctt aataccagta acgataaatt caatccccaa tgccatcagc aatagcccca
    10201 taatacgcgt aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca
    10261 tgcggaacaa tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca
    10321 gatagctaat gctgtgataa cgcgtacccc agacgatggt agaactgatc gcccctggcc
    10381 ccgccatcaa cggcaacgcc aatggcacca caccaatact ttcacgtacc gcggtttctg
    10441 atttttcttg cttgttctgt ttatcctcgc caagcttgcc gctgatcatc gacatcgcta
    10501 tcgtcaccac caggatgccc ccggcgatac ggaacgaatc aattgatata ccaaaaagtt
    10561 gtagaatcgt gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg
    10621 ctgtaaggtt agttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga
    10681 cgggaataat ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa
    10741 cgggaaaatc aaaaagggtc tgaatcacgg ttagctccga agcaaaagcc ggataatgtt
    10801 agccataaat aaggttgaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc
    10861 tcctaactac ttaaaattgc tatcattcgt tattgttatc tagttgtgca aaacatgcta
    10921 atgtagccac caaatcatac tacaatttat taactgttag ctataatggc gaaaagcgat
    10981 gctgaaaggt gtcagctttg caaaaatttg atttggatca cgtaatcagt acccagaagt
    11041 gagtaatctt gcttacgcca cctggaagtg acgcattaga gataataact ctaatgttta
    11101 aactctttta gtaaatcaca gtgagtgtga gcgcgaataa gcttttgatt ttcatcggtt
    11161 aagcaaatca tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa
    11221 gtttaacatt atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc
    11281 gtagagcgtg taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac
    11341 aaaatcttcc gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg
    11401 gccgttgccg aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct
    11461 tctgaatata tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac
    11521 gacacttttg gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg
    11581 accactaacc cgacttcaac tgctatcttc aaatcgctga tcagcctgaa gacccgtaac
    11641 gccattatct tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc
    11701 gttctgcagg ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa
    11761 ccttctgttg aactgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg
    11821 actggtggtc cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggtgta
    11881 ggcgcgggta acactccagt tgttattgat gaaactgctg atatcaaacg tgcagttgca
    11941 tctgtactga tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt
    12001 gttgttgttg actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggcggctat
    12061 ctgttgcagg gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa tggtgcgctg
    12121 aacgcggcta tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta
    12181 ccagaaaaca ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc
    12241 gcacatgaaa aactgtcccc gactctggca atgtaccgcg ctaaagattt cgaagacgcg
    12301 gtagaaaaag cagagaaact ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac
    12361 actgaccagg ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacagct
    12421 cgtatcctga ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa
    12481 ctcgcacctt ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac
    12541 gttggtccga aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg
    12601 tggcacaaac ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat
    12661 gaagtgatta ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac
    12721 aatggttatg ctgatcagat cacttccgta ctgaaagcag caggcgttga aactgaagtc
    12781 ttcttcgaag tagaagcgga cccgaccctg agcatcgttc gtaaaggtgc agaactggca
    12841 aactccttca aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgccgcg
    12901 aagatcatgt gggttatgta cgaacatccg gaaactcact tcgaagagct ggcgctgcgc
    12961 tttatggata tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg
    13021 atcgctgtca ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact
    13081 gacgacgcta ctggtcagaa atacccgctg gcggactatg cgctgacccc ggatatggcg
    13141 attgtcgacg ccaacctggt tatggacatg ccgaagtccc tgtgtgcttt cggtggtctg
    13201 gacgcagtaa ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat
    13261 ggtcaggctc tgcaggcact gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa
    13321 gggtctaaaa atccggtagc gcgtgaacgt gttcacagtg cagcaactat cgcgggtatc
    13381 gcatttgcga acgccttcct gggggtatgt cactcaatgg cgcacaaact gggttcccag
    13441 ttccatattc cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat
    13501 gcgaacgaca acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc
    13561 cgtcgttatg ctgaaattgc cgaccacctg ggtctgagcg caccgggcga ccgtactgct
    13621 gctaagatcg agaaactgct ggcatggctg gaaacgctga aagctgaact gggtattccg
    13681 aaatctatcc gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt ggataaactg
    13741 tctgaagatg cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc
    13801 gagctgaaac agattctgct ggatacctac tacggtcgtg attatgtaga aggtgaaact
    13861 gcagcgaaaa aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa
    13921 tcagtagcgc tgtctggcaa tataaacggc cccttc
//
LOCUS       DADMGC010000107        13954 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.107, whole genome shotgun sequence.
ACCESSION   DADMGC010000107 DADMGC010000000
VERSION     DADMGC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.107"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..494)
                     /locus_tag="JNZ73_003080"
     CDS             complement(<1..494)
                     /locus_tag="JNZ73_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733282.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(525..1217)
                     /gene="fimC"
                     /locus_tag="JNZ73_003081"
     CDS             complement(525..1217)
                     /gene="fimC"
                     /locus_tag="JNZ73_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAY8733283.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1418..1960)
                     /gene="fimA"
                     /locus_tag="JNZ73_003082"
     CDS             complement(1418..1960)
                     /gene="fimA"
                     /locus_tag="JNZ73_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein subunit FimA"
                     /protein_id="HAY8733284.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2441..3307
                     /gene="folD"
                     /locus_tag="JNZ73_003083"
     CDS             2441..3307
                     /gene="folD"
                     /locus_tag="JNZ73_003083"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HAY8733285.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3309..3521
                     /gene="ybcJ"
                     /locus_tag="JNZ73_003084"
     CDS             3309..3521
                     /gene="ybcJ"
                     /locus_tag="JNZ73_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HAY8733286.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3629..4150
                     /locus_tag="JNZ73_003085"
     CDS             3629..4150
                     /locus_tag="JNZ73_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY8733287.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(4186..5571)
                     /gene="cysS"
                     /locus_tag="JNZ73_003086"
     CDS             complement(4186..5571)
                     /gene="cysS"
                     /locus_tag="JNZ73_003086"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAY8733288.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5745..6239
                     /gene="ppiB"
                     /locus_tag="JNZ73_003087"
     CDS             5745..6239
                     /gene="ppiB"
                     /locus_tag="JNZ73_003087"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HAY8733289.1"
                     /translation="MVTFHTNHGDIVIKTFDNKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6242..6964
                     /gene="lpxH"
                     /locus_tag="JNZ73_003088"
     CDS             6242..6964
                     /gene="lpxH"
                     /locus_tag="JNZ73_003088"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HAY8733290.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7082..7591
                     /gene="purE"
                     /locus_tag="JNZ73_003089"
     CDS             7082..7591
                     /gene="purE"
                     /locus_tag="JNZ73_003089"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAY8733291.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7588..8655
                     /gene="purK"
                     /locus_tag="JNZ73_003090"
     CDS             7588..8655
                     /gene="purK"
                     /locus_tag="JNZ73_003090"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAY8733292.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8792..9685)
                     /locus_tag="JNZ73_003091"
     CDS             complement(8792..9685)
                     /locus_tag="JNZ73_003091"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAY8733293.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRWVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9682..10329)
                     /locus_tag="JNZ73_003092"
                     /pseudo
     CDS             complement(9682..10329)
                     /locus_tag="JNZ73_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10332..11564
                     /gene="allC"
                     /locus_tag="JNZ73_003093"
     CDS             10332..11564
                     /gene="allC"
                     /locus_tag="JNZ73_003093"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HAY8733294.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11575..12360
                     /gene="allE"
                     /locus_tag="JNZ73_003094"
     CDS             11575..12360
                     /gene="allE"
                     /locus_tag="JNZ73_003094"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HAY8733295.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(12588..13733)
                     /gene="glxK"
                     /locus_tag="JNZ73_003095"
     CDS             complement(12588..13733)
                     /gene="glxK"
                     /locus_tag="JNZ73_003095"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HAY8733296.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANSHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDALFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(13755..>13954)
                     /locus_tag="JNZ73_003096"
     CDS             complement(13755..>13954)
                     /locus_tag="JNZ73_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HAY8733297.1"
                     /translation="QITFTERNIYRLALPLFVGIFLMALPPVYLQDLPLTLRPLLSNG
                     LLVGILLAVLMDNLIPWERIE"
BASE COUNT         3386 a         3680 c         3496 g         3392 t
ORIGIN      
        1 tgagcgctac tcaacaacgc tatctgagga atagtgatgt tcaggcgcat cgctgcaaaa
       61 tcaaagttaa ttgtggcatc aggtacagct ttcagtaaat tgatgcattt attgttttgc
      121 tgctgggcta actcgggaaa tgcactgcta ttaaggccaa ttcgttcaag gagtacctgg
      181 ttaaaacagg gcattaaccc acctgattta tcacctgtat tttctgttgt cgattcaaat
      241 acgatatcct gtgaaccaat gaactcatca ttacgccaga gatccacccg ataaacgccc
      301 gcaggttgat gattacctct ctcaaagcgc gataaatcag caacggatgt gccattctct
      361 aataaaaatg ccgggttgaa ataactttcc gcatagctta aaaaggaggg taaaatggca
      421 agagatgaat aacaaattcc ggccagacac caggtatacc tctgcggaat atccgtagta
      481 gtgggtattt tcataattag ctctgatatt gtcagatgat tcggttagtt taagttcact
      541 tctctgaccg gggttactga accataatca ttaacggtct gaaatttcag ctttccctgc
      601 actcctgagg gtaagggaat ttgtgaacta actctgggtg ccgccatagc attccccaac
      661 ttttggctgc ccacttgcag gttaacgagg gtgacataaa aaggtgatgg attatgaaca
      721 ttgatatagt taccggatcg cgaaaacttg agtgtgtccg gcgcttctgc gggtaattct
      781 tgtaattgaa ttggacggag aaataatttc atgcgcgata aaatcgccag ttgcaaaaca
      841 ttcctgccgt tcaatgcatt tttatctacc gaagggatcg tcttaacatt catccagaac
      901 agagactcac gatctgccgc cagcggtgga ccggtgtaaa taatacgcaa agtattttcg
      961 cttttgggtt cactaacaaa tagtggcggt gtaatgatga atgacttttc ttttacaccg
     1021 ctgctgtttt caatccacga attgaccaga aagagctcat tggtatggct atttttaatc
     1081 cataccgcag tctgtttagc atcagcggga taaataatac gcgtggcacc taatgcgatc
     1141 cctccggcag catgggcgct ggccgaaatg attaaataaa atatcatgag catcaataac
     1201 tttattttag tcatcatagt taatacgcct tgtgtattcc tgacggcaaa agattgcttt
     1261 ggcttcttac aacacatcat tcccttttca tttaataaag cggcatgata caaagtgaac
     1321 gtggtttcga tttacattgc caccataccg cttcagacaa ccggtacatt actttgttat
     1381 aagacgtgat atataattca aaacaacgtg gttttgatta ttcgtatctc ataataaaag
     1441 tcgcgtcagc atttgcttgc cctgctgatg cacttgtacc ggtgccttta taacgtgcag
     1501 aaaaatgaag aacgttggtc ccggggatca agttctggtt ggttgagaag ctattcccat
     1561 caggtttgag aattgcggat gtattatcaa gtatttcaat accgacaccc gttgctgttg
     1621 ttgtatttgt actggatgca atggccaata aattatcatt gattgcatca gcctgaccag
     1681 aaaatgcaac ggcagcatta gcggcaacaa caggatcgca gtcgttcaac tgaatggtga
     1741 atggaattaa tgcagaggta ttaccaacag cattgaaaat atcggtacga tattgaccga
     1801 gtgtgacaac ctggtctgcc gagtcagtat tcactgaaca ggcagcattc accagttcgc
     1861 cttcaaagtg aattgtaccg ccatctacaa cggcagcata actacccgta gcggcgaata
     1921 gtgcggcagc cagcgcagac gaaataaatc ttaatttcat atatattcct tcaatctcat
     1981 ttatcgactc cacatccgta tataaccgat tactttattt aagacactga tagtagtaaa
     2041 attccttttt atcctctaag aatgtcttaa ttgaaaatat gcactctatt ctaaaaaata
     2101 gagagccccg ttagatgaat acttccgcgc aaaatatatt caacacaaat atagacctga
     2161 agcggtaaat taccaggctg aaaattcttt ttatattgtc aggtatttct taaattatct
     2221 taatccttaa acaaggaaat aaatcagttc cagatttaca acgccatcat gtacgaaaaa
     2281 tgaagctttc aatctcagcg acctcagcga cggtgcgcct aaccttcgca agaggtcgct
     2341 tcacgcgata aatctgaaac gaaacctgac agcgcgcccc gcttctgaca aaatagacgc
     2401 atccccttcg acctacgtaa cagatggaat cctctctctg atggcagcaa agattattga
     2461 cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct caaaaagttc aggctcgtat
     2521 tgcagccgga ctgcgggcac caggactggc cgttgtgctg gtgggtagta accctgcatc
     2581 gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa gtcgggttcg tctcccgctc
     2641 ttatgacctc ccggaaacca ccagcgaagc ggagctgctg gagcttatcg atgcgctgaa
     2701 tgccgacaac accatcgatg gcattctggt tcaactgccg ttaccggcgg gtattgataa
     2761 cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg gacggtttcc atccttacaa
     2821 cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc tgcaccccgc gcggtatcgt
     2881 cacgctgctt gagcgttaca acattgatac cttcggcctc aacgccgtgg tgattggcgc
     2941 atcgaatatc gtcggtcgcc caatgagcat ggaactgttg ctcgcaggtt gcaccaccac
     3001 cgtgactcac cgcttcacta aaaatctgcg tcatcacgta gaaaatgccg atctgttgat
     3061 cgttgccgtt ggcaagccag gctttattcc cggtgactgg atcaaagaag gcgcaattgt
     3121 gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt gtgggcgacg tcgtgtttga
     3181 agacgcggct aaacgcgcct catacattac gcctgttccc ggcggcgttg gcccgatgac
     3241 ggttgccacg ctgattgaaa acacgctaca ggcgtgcgtt gaatatcatg atccacaggg
     3301 tgagtaacat ggcgacattt tctttaggta aacatccgca cgttgagctg tgcgacttgc
     3361 tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa aatcgcgatt gccgaaggcc
     3421 aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg caaaatcgtc gccggtcaga
     3481 cagtgagttt tgcaggacac agcgtacagg ttgttgcctg atccctttcc cggcctgtat
     3541 caacaggccg gttctctttt cagacttttg ccctctacgc cccgttcatc catgctgata
     3601 gcatcgacat tttgccgaag cgaccatcat gccaaccgtt attacgcacg ccgccgttcc
     3661 cctttgcatt ggtttagggt tgggttcgaa agtcatccct ccgcgtctgt tatttgccgg
     3721 aatcatccta gcgatgctgc ccgatgccga cgtattgtcg tttaaatttg gcgttgctta
     3781 cggcaatgtt tttggtcatc gcgggtttac ccattcgctg gtgtttgcgt ttgttgtccc
     3841 gctcttatgt gtgctgattg gacgtcgatg gttcagggct gggctgattc gctgctggct
     3901 gtttttaacc gtctcgttgc tatcgcacag cttactggat tcggtaacca ctggcggtaa
     3961 gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt ttcgctccct ggcaggtgat
     4021 taaagtcgcg ccgtttgcat tgtctcgtta caccacagcg tacgggcatc aggtgattat
     4081 ttccgaattg atgtgggtgt ggttgccggg gatgctactg atgggaatgt tgtggtggcg
     4141 cagacgataa ccggatgcga aaactcgcat ccggcaattg cgcaattact tacgacgcca
     4201 ggtggtccct tgcgggccat cttccagcac gatccccatc tcgttaagac ggtcacgtgc
     4261 cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc agacgctgtt gaattaacgc
     4321 ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg ctttgcagga acgcttccgg
     4381 ttcttgctcc agcaggccca atacagcgga aagtttacgc aggtgagatg ccattgcatt
     4441 cgccgctgcc atatcttctg ctttcagacg gttcacttca cgcgccatat caaacagcac
     4501 ggaataggct tccggggtgt tgaaatcgtc gtccatcgct tcaataaagc gcgcttcaaa
     4561 cgcttcgcca ccggcaggcg caacggtttt atctgtgccg cgcagcgcag tgtagaggcg
     4621 ctccagcgcc gcacgcgcct gcttcaggtt ctcttcgctg tagttcagct ggctgcgata
     4681 gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg tagtatttca gcacatcgcg
     4741 cacggtaaag aagttaccca gcgatttgga catcttctcg cggtcaacca tcaccattcc
     4801 ggagtgcatc cagtagttca catactgacc atcatgggca caggtggact gcgcgatttc
     4861 gttctcgtgg tgcgggaaca tcaggtctga accgccgccg tggatatcaa agtggttacc
     4921 cagctgcttg cagttcatgg ccgaacattc aatgtgccag ccaggacgac ccgcgcccca
     4981 cggagacggc cagctcggtt cgccctcttt cgacatcttc cacagaacga agtccatcgg
     5041 gttgcgtttg tcatcaacca cgtcaacgcg cgcgccagcc tgcagctggt cgagatcctg
     5101 acgcgacagc acgccataag ttggatcggt cgggacgtcg aacatcacgt cgccgttgtc
     5161 cgccacataa gcgtgacctt tggcgatcag ttgttcagtg agttcaataa tttctgcgat
     5221 atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg ttcaaagcat caaaatcttt
     5281 gtgcatttcg gcaatcatgc gatccaccag cgccacaaag ctttcgccat tttcattggc
     5341 gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc acatacttca gtttatagcc
     5401 gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca aaggtacgcc cgtgaccgat
     5461 atgacagaga tcgtaaacgg tgattccaca cacgtacatg ccgacttccc cggcgtgaat
     5521 aggcttaaat tcctcttttt ggcgtgtcag agtattgaag atttttagca tcgaagattc
     5581 cgtttagaca tgtgtgggta attgagttgc gtataatacc catatttccc gcgcgaatca
     5641 gcatacattg cgtgatgatc cgatcgtgcg gttatgctat aacaccaccc tatatatgac
     5701 ccgaactggg ttgaagcacc aatcaaacgg aacaggatgc aaaaatggtt actttccaca
     5761 ccaatcacgg cgatattgtc atcaaaactt ttgacaataa agcacctgaa acagttaaaa
     5821 acttcctgga ctactgccgc gaaggttttt acaacaacac cattttccac cgtgttatca
     5881 acggctttat gattcagggc ggcggttttg aacctggcat gaaacaaaaa gccaccaaag
     5941 aaccgatcaa aaacgaagcc aacaacggcc tgaaaaatac ccgtggtacg ctggcaatgg
     6001 cacgtactca ggctccgcac tctgcaactg cacagttctt catcaacgtg gttgataacg
     6061 acttcctgaa cttctctggc gaaagcctgc aaggttgggg ctactgcgtg tttgctgaag
     6121 tggttgacgg catggacgtg gtagacaaaa tcaaaggtgt tgcaaccggt cgtaacggta
     6181 tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag cgtgaccgtt agcgagtaat
     6241 cgtggcgaca ctctttattg cagatcttca tctctgcgtg gaagaaccgg cgatcaccgc
     6301 cggttttctg cgttttttag cgggggaagc ccgcaaggcc gacgcgctgt atattcttgg
     6361 cgatctgttt gaagcatgga ttggcgacga cgatcccaac ccactccatc gccagatggc
     6421 ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt tatttcattc atggcaaccg
     6481 tgattttctg ctcggcaaac gctttgcccg tgaaagcggc atgacattat tgccggaaga
     6541 aaaggtgctc gaactttatg gtcgccgggt gttgattatg catggcgaca cgctgtgcac
     6601 cgatgacgcg ggttatcagg cttttcgcgc caaagtccac aaaccctggc tgcaaatgct
     6661 attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc gcgcgaatgc gcgcgaacag
     6721 caaagaagcc aacagcagta aatcgctggc gatcatggac gttaaccaaa acgctgtggt
     6781 cagtgcgatg gaaaaacatc aggtgcaatg gctaatccac gggcataccc atcgcccggc
     6841 ggtgcatgaa cttatcgcca accagcaacc tgcttttcgc gtggtactgg gtgcctggca
     6901 tacggaaggt tcgatggtga aagtcacggc ggatgacgtt gagctgattc attttccgtt
     6961 ttaaaaaacc cacaactttg ctgatttcac agccacgcaa ccgttttcct tgctctcttt
     7021 ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc accacaggag ttttaagacg
     7081 catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg atggggtcca aaagcgactg
     7141 ggctaccatg cagttcgccg tcgaaatctt cgaaatcctg aatgtcccgc accacgttga
     7201 agtggtttct gctcaccgca cccccgataa actgttcagc ttcgccgaaa gcgccgaaga
     7261 gaacggttat caggtgatta ttgcgggcgc aggcggcgca gcgcacctgc caggcatgat
     7321 tgccgccaaa acgctggtgc cggtgctggg cgtgccagta cagagcgccg cactgagcgg
     7381 tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc attccggtgg gtacgctggc
     7441 gattggtaaa gctggcgcgg caaacgcggc attactggca gcacaaattc tcgcgactca
     7501 cgataaagag ctacaccagc gtctgaatgg ctggcgcaaa gcccagaccg acgaagtgct
     7561 ggaaaacccg gacccgcgag gtgcggcatg aaacaggttt gcgtcctcgg taacgggcag
     7621 ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca ttgctgtctg gccggtcggg
     7681 ctggacgctg aaccggcggc ggtgcctttt caacaaagcg tgattaccgc tgagatcgaa
     7741 cgctggccgg aaaccgcatt aacccgcgag ctggcgcgtc atccggcctt tgtgaaccgc
     7801 gatgtgttcc cgattattgc tgaccgtctg actcagaaac agcttttcga taagctccac
     7861 ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg agtggcctgc ggtgtttgat
     7921 cgtttaggtg aactggcgat tgttaagcgt cgcactggtg gctatgacgg tcgcggtcaa
     7981 tggagtttac gcgccaatga aaccgaacag ttaccggcag agtgttacgg tgaatgtatt
     8041 gtcgagcagg gcattaactt ctctggtgaa gtatcgctgg ttggcgcgcg cggctttgat
     8101 ggcagcaccg tgttttatcc gctgacgcat aacctgcatc aggacggtat tttgcgcacc
     8161 agcgtcgctt ttccgcaggc caacgcacag cagcaggcgc aagccgaaga gatgctgtcg
     8221 gcgattatgc aggagctggg ctatgtgggc gtgatggcga tggagtgttt tgtcaccccg
     8281 caaggtctgc tgatcaacga actggctccg cgtgtgcata acagcggtca ctggacacaa
     8341 aacggtgcca gcatcagcca gtttgagctg catctgcggg cgattaccga tctgccgtta
     8401 ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc tgattggtag cgatgtgaat
     8461 tatgactggc tgaaattgcc gctggtgcat ctgcactggt acgacaaaga agtccgtccg
     8521 gggcgtaaag tggggcatct gaatttgacc gacagcgaca catcgcgtct gactgcgacg
     8581 ctggaagcct taatcccgct gctgccgcca gagtatgcca gcggcgtgat ttgggcgcag
     8641 agtaagttca gttaagcagg tgctcatttg attgccggat gcggcgtaaa cgccttatcc
     8701 ggcttaccgc tccggtaccc attgtaggcc tgataagacg cgtcaagcgt cgcatcaggc
     8761 atggtgcacc gattgccgga tgctctcatc actacagcga aatacaggtc cccgcttcgc
     8821 ccgccagcgt ctcttcaatt cgcgataacg ccccaatcca cgcgggttta ctacggcttc
     8881 tgacataacc actcaccgcc gttaccttcg gccccatcga accatcggct ttggcaaatg
     8941 gcgctaactc atccggtgtg gcatggcgaa tggcacgttg ctgcggcgtt ccccagtttt
     9001 catataccgc atcagcatcg gtgaggatca ccagtccatc ggcattaatc tgctcggcga
     9061 gcaacgcagc ggcgagatct ttatcaatca ccgcttcact ccctgcccca tcttccgtca
     9121 caggcacacc gccgccgcca ctgcaaatca ccacatgccc ctctttgagc aacaactcga
     9181 tggcttcgct gtcgagaatt ttacgcggtt gcggagacgc caccacccag cgcaaatatt
     9241 taccatcacg tttcatctgc cagccgtaag ccgcttccag tgcctcttgt tcttctggct
     9301 gataaaccgg accaataaat ttctctggct gcaaaaacgc cggatcatca ggcgaaacct
     9361 caatgcgcgt cagcaccgtc gtaacaggcg gcatctgcgg ctgtgcgctc aagctctgcg
     9421 ccagcatata gccaatcatc ccctggcttt ccgccaccag cacatccagc ggatacggtt
     9481 ctacctcttt ccacgccaga ttctgtaacg ccagcagccc cacctgcggc ccgttgccgt
     9541 gaacaatcgc cagccgataa gaacgggcca ggcgtgccag cgcgggtaca gcactggcga
     9601 tattgcgata ttgattttcc gccgtcagcg cctcaccgcg ctggagtaag gcgttgcccc
     9661 caagagctac aaccagtgtt ttcatggttt tccttgtaat aattgttggc caagccagaa
     9721 ccccagcagc gtatctgccc ctgacgtatg ccccagcgcc agcagcgaat caatcgcaac
     9781 agcggtacgt ttcgggcaac tcagagcatg gacaaagtgc aggagtggcg aggcgaaata
     9841 tccttgcgcg gcataacgta aataactgac gctcaccgct gtggtaacga gttgaagatt
     9901 gtcggaacag gcaaaaaacg ggcgaccggc gcgcgcatct aaagcgccat aataccaggc
     9961 cgccagcagc attccgctca acgtgtcatc atgacttggc gttaatccgg ggcctttacc
    10021 cagccagtgc cgccagtcgg tcttaacgcc attgagcgcg gcctgaaaac agtgacgaaa
    10081 ctggcgtaat tcagcgggca gcggatcgct tgccgccatc gccagtggcc cgaaaagtcc
    10141 ggtttcctcc gcgcgttgca tccatgcagc tgcaagcggt tgaggatgcg aaggcggcgt
    10201 aatacgcagc aaacaataac gctgtggctg tttaaccgtg aaacgcccga ggcgaatccc
    10261 tttagccaca acctgtggtc gttcattgcc gcataatcca ccgcataacg catcgaattg
    10321 cgtgcggcga aattacacat tcccgtcaag ctatagaaga aacgctgccc tggctttcct
    10381 cttttggcgc tgacccaacg ggtgggatga cccgtttact ttattcgccg gaatggctgg
    10441 aaacccagca gcaatttaaa aaaagaatgg cagcaagtgg gctggaaaca cgtttcgatg
    10501 aagtggggaa tttatacggt cgcctgagtg gcaccgaata tccacaggaa gtggttctga
    10561 gcggttcgca tatcgatacc gtggttaacg gcggtaacct cgacgggcaa ttcggcgcgc
    10621 tggcggcgtg gctggcaatt gactggttga aaacgcaata cggcgcaccg ttacgtacgg
    10681 tcgaagtggt ggcgatggca gaagaagaag gcagccgctt cccgtatgtc ttctggggca
    10741 gtaaaaatat ctttgggctg gcgaatcctg acgacgtgcg gaatatctgt gatgccaaag
    10801 gaaatagttt tgccgatgcg atgaaggctt gcggatttac tctgcctaac gccccactaa
    10861 ctccgcgtca ggatataaaa gcctttgttg aactgcatat tgaacagggc tgtgtgctgg
    10921 aaagtaatgg gcaatcaatt ggcgtggtga atgcaattgt cgggcagcgt cgctatacgg
    10981 tgacgctgaa cggcgagtca aaccatgcag gcaccacgcc gatgggttat cgtcgtgata
    11041 cggtttacgc tttcagtcgc atttgccatc agtcgatcga aaaagcgaaa aagatgggcg
    11101 atccgctggt tctgaccttt ggcaaagtag agccgcgccc gaatacggta aatgtggtgc
    11161 cgggtaaaac cacgttcacc attgattgtc gtcataccga cgctgccgtg ctgcgcgatt
    11221 tcacccaaca gttagaaaac gacatgcggg cgatttgcga tgaaatggac attggtattg
    11281 atatcgattt atggatggac gaagaacccg tgccgatgaa taaggagctg gtcgccaccc
    11341 tgacagaatt gtgtgaaaga gaaaaactga attaccgggt gatgcacagt ggtgccgggc
    11401 acgacgcgca aattttcgcg cctcgcgtac caacctgcat gatttttatc cccagcatca
    11461 acgggatcag ccataacccg gcggaacgca ccaatattac tgaccttgcc gaaggggtca
    11521 aaacgttggc actcatgctt tatcaacttg cctggcagaa ataaggagtc ataaatggga
    11581 tatttaaata acgtcaccgg ttaccgcgaa gatttactgg ctaaccgtgc gattgttaaa
    11641 cacggtaatt tcgcactgtt aaccccggat ggtctggtaa aaaatattat tccgggcttt
    11701 gaaaattgtg acgcgacaat cctctccacg ccaaagctgg gtgcctcttt tgttgattat
    11761 ctggtcacac tgcatcaaaa cggtggcaac caacagggct tcggtggcga aggcattgaa
    11821 acgttcctgt atgtgatctc tggaaatatc actgccaaag ccgaaggcaa aacatttgcc
    11881 ttaagcgaag gtggctatct ttattgccca ccaggctcct taatgacgtt tgttaacgcc
    11941 caggccgaag acagccaaat ctttttatat aagcgccgct atgttccggt agaaggccat
    12001 gcaccgtggc tggtttctgg caatgccagc gaactggaac gcattcacta tgaaggtatg
    12061 gatgatgtga tcctgctgga ttttctgccc aaagagttag gttttgatat gaacatgcat
    12121 atcctctctt ttgcaccagg tgccagccac ggttatatcg aaacacacgt tcaggaacat
    12181 ggtgcctata ttctttccgg tcagggggtt tataacctcg acaataactg gatcccggtg
    12241 aaaaaaggcg attacatctt tatgggcgct tattctttac aggctggtta tggcgtaggg
    12301 cgtggtgaag cgttcagcta tatttactcg aaagattgca accgcgacgt agagatttaa
    12361 gtcataccac tcgcctgatt atttttagct atgttggatt ttgccacggt ttataccgtg
    12421 gctttttttg taacattgcc tgatgcgctt cgcttatcag gcctacatga cctctgcaat
    12481 atatagaatt tgcgcgattt tcaggaagga tatagtactc atgccgcatc cggcatgagt
    12541 actgcgctct tatacgaaat aaatcgcatc cgctttaaag gcaagagtca gtttttaatt
    12601 ccctgaccta ttttaatggc gcaggcaata tttcgcgcgc tgttgaagag attggtttca
    12661 ccgctggcga gcacttcggc taaaggtgcc agacgaggca aaatgctgaa tagcgcgtca
    12721 atgccgtact ggtgcaccac ttccacgcca tcacccaata caccagcaat cccaatcacc
    12781 ggtacattaa actgcttcgc caccgacgca acgcccagcg gcgctttacc gcctgccgtt
    12841 tgcgagtcaa tgcgcccttc cccggtgatc accagtgctg ccccctgcac cgcctgcgca
    12901 agattgaccg cattcaacac aatctcaatg cccggtttaa tatccgcatt gagaaatacc
    12961 gccgccgcaa tccccatacc gcccgcagcg ccgccgcccg ccatctgaca gacattaatt
    13021 tcagtttgct gttgtaaaac acgggcgtaa ttttgcagcc cctgttccag ctcttcaacc
    13081 atctccggcg ttgccccttt ttgcgggcca aataccgccg ccgcgccgcg tgcccctacc
    13141 agcgggttat caacgtcaca tgccacttca atatggctgt tcgccagacg cggatcgcat
    13201 tcctccatct caatgcttgc cacgcgcgct aaattgccgc cgttagccgc cagcacctgg
    13261 ccgtcggcat caaggaaacg cacaccaagc gcctgcgcca tgcccatacc gccgtcgacc
    13321 gtcgcactgc cgccaatgcc gagaataata tggcgaatgc cgttatccag cgcatggcga
    13381 attaactccc ccgtaccaaa actggaggcc agcaacggat tacgcttttc aggcgcgacc
    13441 agcatcaggc cacttgctgc cgccatctca atcaccgctg ttttcccgtc tccggtaagg
    13501 ccataaaaag cattcacttt ttcgcccatc ggcccgcaga cttcaagcgt cacgatgttg
    13561 ccacccgtcg cggcgaccat cgcatccacc gtcccttcgc cgccatccgc tatcggcaaa
    13621 cagatatagt gcgcatcggg aaagagggtc gaaaacccgg ctttaattgc ctgacaacat
    13681 ttttctgcac ttaagctctc cttaaaagag tctggcgcaa tgacaatctt cattatcaat
    13741 cctttttcaa caaattattc gatgcgttcc cacggaataa gattatccat aagaacagcc
    13801 agtaaaattc cgaccagtaa gccgttactg agcagaggac gaagcgttaa tggaaggtct
    13861 tgcaaataca caggcggtaa tgccattaaa aatatgccga caaataacgg caatgcgagt
    13921 cgataaatat tgcgctcggt aaacgttatt tgct
//
LOCUS       DADMGC010000108        13817 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.108, whole genome shotgun sequence.
ACCESSION   DADMGC010000108 DADMGC010000000
VERSION     DADMGC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.108"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            1..454
                     /locus_tag="JNZ73_003097"
                     /pseudo
     CDS             1..454
                     /locus_tag="JNZ73_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            514..621
                     /locus_tag="JNZ73_003098"
     CDS             514..621
                     /locus_tag="JNZ73_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HAY8733298.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(602..1333)
                     /gene="yehW"
                     /locus_tag="JNZ73_003099"
     CDS             complement(602..1333)
                     /gene="yehW"
                     /locus_tag="JNZ73_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HAY8733299.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="JNZ73_003100"
                     /pseudo
     CDS             complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="JNZ73_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
     gene            complement(2257..3415)
                     /gene="yehY"
                     /locus_tag="JNZ73_003101"
                     /pseudo
     CDS             complement(2257..3415)
                     /gene="yehY"
                     /locus_tag="JNZ73_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="JNZ73_003102"
                     /pseudo
     CDS             complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="JNZ73_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
     gene            complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="JNZ73_003103"
     CDS             complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="JNZ73_003103"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HAY8733300.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MMVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7084..8799
                     /gene="dld"
                     /locus_tag="JNZ73_003104"
     CDS             7084..8799
                     /gene="dld"
                     /locus_tag="JNZ73_003104"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY8733301.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="JNZ73_003105"
     CDS             complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="JNZ73_003105"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HAY8733302.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9943..10530)
                     /locus_tag="JNZ73_003106"
     CDS             complement(9943..10530)
                     /locus_tag="JNZ73_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HAY8733303.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            10700..11278
                     /locus_tag="JNZ73_003107"
     CDS             10700..11278
                     /locus_tag="JNZ73_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8733304.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(11395..12156)
                     /locus_tag="JNZ73_003108"
                     /pseudo
     CDS             complement(11395..12156)
                     /locus_tag="JNZ73_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(12209..13735)
                     /gene="mdtQ"
                     /locus_tag="JNZ73_003109"
     CDS             complement(12209..13735)
                     /gene="mdtQ"
                     /locus_tag="JNZ73_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HAY8733305.1"
                     /translation="MLLMYCHAKLKNISQHTVISAHRFLPDYSPMNRDSFYPAIACFP
                     LLLMLAGCAPMHETRQALSQQTPAAQVDTALPTALKNGWPDSQWWLEYHDNQLTSLIN
                     NALQNAPDMQVAEQRIQLAEAQAKAVATQDGPQIDFSADMERQKMSAEGLMGPFALND
                     PAAGTTGPWYTNGTFGLTAGWHLDIWGKNRAEVTARLGTVKARAAEREQTRQLLAGSV
                     ARLYWEWQTQAALNTVLQQIEKEQNTIIATDRQLYQNGITSSVDGMETDINASKTRQQ
                     LNDVVGKMKIIEARLNALTNHQTKSLKLKPVALPKVASQLPDELGYSLLARRADLQAA
                     HWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTLPIFD
                     SGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIERDAL
                     RVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDASIRLTGALGGGYKR
                     "
BASE COUNT         3286 a         3875 c         3492 g         3164 t
ORIGIN      
        1 ggcaatctac atagcctgcg aacgtggatc aaagagcgcg gtcaggatta ccccgcccag
       61 acactcacca cacatctgtt tattcctctg cgccgacggc ttcagtgcca acaaccgact
      121 ctccaggcgc tgctggcgat cctcgacggc gtactgatca actacatcgc catttgtctg
      181 gcttcggcac gtaaaaaaca gggtaaagat gcgctggtgg ttggctggaa tattcaggat
      241 accacccgtc tgtggctgga gggctggatt gccagtcaac aaggatggcg cattgatgtc
      301 ctcgctcgcc cactcgctca atcaactacg ccctgaacta ttcgaaggcc gtacattgct
      361 ggtgtggtgc ggtgaaaatc gaacctccgc ccaacagcag caactcacca gttggcaaga
      421 acaaggccat gatattttcc cactcggtat ttaatgattc gttaacaaat gcgctttact
      481 gtacaatcct ttcgttaaca taaggagtgc attatgcgca tagctaaaat tggggtcatc
      541 gccctgttcc tgtttatggc gttaggcgga attggtggcg tcatgctcgc aggttatacc
      601 tttattttgc gtgctggcta agcgcctgca ccagcctttc aaacaggcgg tctgcgatga
      661 tcgccgccag tgccaccagt aacgccccct ggatcacata cgcggtatta aatccgctaa
      721 gcccgatgat gatgggcgta cccagcgtac tggcccctac cgttgaggcg atcgtcgccg
      781 taccaatgtt gataatcacc gaagttcgca cgcccgccag aatcaccgga gccgccagcg
      841 gtagctcgac cttacgcagt cgctgacctc gactcattcc catacctttc gcaacttctg
      901 tcacgctggg atcaatcgct cccagcccgg caagtgtcac ctgcaggatg ggcagcacac
      961 cgtaaaggat caaggcgata atcgctggtt gcagaccaaa gccgatcacc ggaacggcaa
     1021 tcgccagcac tgcgacaggc ggaaaagtct gtccaacggc ggcaatagtt tccaccagtg
     1081 ggcgaaattc cgcgccccac gggcgagtaa cagcaattcc ggcacctgtg ccaatgatca
     1141 ccgcaaacaa actcgaaatt cccaccagcc agaaatgagc cagtgccaga gctgcaaaac
     1201 tttcttgctg ataaacgggt cgtggcagtt gtgggaacaa ggcagcaaac agcggctggc
     1261 tgtaaggcag ccagaaaatc agcgccacaa aaagagcaat gagccagaac agcggatcgc
     1321 gcaacatctt catacgctta cgcctccacc agcagatcct gaaaatgcag cgtgccgcaa
     1381 ggctggccct gcgtgttcac caccggcagc acctcgcatc cccgcgcgac aaacagggag
     1441 agcgcatcgc gtagcgtcat ctcttctgcc agtgcctcac cttctgcccg ttcttcgcga
     1501 cgcacgtaat ccgccacgct acgtaacgaa agcaggtgca cccccagttc actacgtcca
     1561 aaaaactggc ggacaaaatc attagccgga cgagtcagca tcgtcagcgg attcccctgc
     1621 tgcaccactt caccgtgatc catcaatacc agatgttctg ccagccgtag cgcctcatca
     1681 atatcatgag tgaccagcac aatagtgcgc cccagcaaac ggtgaatgcg cgtcatctct
     1741 tgttgcaacg cgccgcgcgt taccgggtcc agtgcgccaa aaggttcatc catcagtaag
     1801 acttgcggat cggcagccag tgcacgcgcc actcccacac gttgctactg accaccggaa
     1861 agctgatgcg gataacgctc acgcaaattt gactccagcc ccagtagcgc cattaattcg
     1921 tcgatacgat cgtcaatccg cgcccgcgac catttttgta attgcggcac ggtagcgatg
     1981 ttttgcgcca cgctccagtg ggggaacagg ccaatagatt gaatggcata gcccatccgg
     2041 cggcgcaact ccagtactgg cagcgagcga atttcttctc cggcaaagcg gatcactccg
     2101 ctgtcatgct ccaccaggcg gttaatcatt ttcagggtgg tggatttgcc ggagccagat
     2161 gtgccaatca gcaccgaaaa actcccttcc tgaaaattga gattgagatc gttaacggct
     2221 ttttgtgcgc cgaacagttt gctgacatgg ctaaattcaa tcattacgtt tcaccttcag
     2281 cagtgcgata agcaaatcga acagcgcgtc ggtaagcacc gccagaacaa ttaccgggat
     2341 cacccccagc aacactaaat caatggcgct gcttagcagc ccctggaaaa ccagcgcacc
     2401 aaaaccgcct gcgccgatta acgccgcaat caccgccata cctacagttt gcaccatcac
     2461 cacccgcagg ctgcgcagaa ataccggtaa cgccagcggt aactgaacat gcaggaaacg
     2521 ctgcgccccg ctcatcccca tcgctctggc gctctccagc acatcgcgcg ggatctggtt
     2581 caaaccgact accacgccgc gcaccagcgg cagcaaggca tagagcacca gcgcaatcag
     2641 tgcgggtgtc attccggttc ctgctatgcc gagcttcccc agccacggaa aggccgtaac
     2701 cagcgcggca agcggcgcaa tcaacaggcc aaagagcgcc accgaaggca cggtctgaat
     2761 gacattgagc agagaaaaaa ttgccccctg ccgcgcagtg gaaaagtagc accagatgcc
     2821 caacggcaca ccaatcacta acgcaggcag caccgcacca aacagcaacg tcagatgttg
     2881 tgccagcgcg tcgtcaaaca catcctgacg gttggcgtat tctttcattt agtgagagat
     2941 cgttaagcgt gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca
     3001 accagcgcca cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg
     3061 ccagcgccgc cgccagccag aaaccactgc cgaggctggt acgcgccagc gcactgccat
     3121 tttgcgccag ttgggtcgcc gcctttccag ctccccacac caacaatacg aagacgaatt
     3181 gcgccagaat gagtgcaaaa atgctgcctt ttttagcggg gacaaaacag gctgtcagcc
     3241 aggcgcaacc aacgcccacc agcatccaga tcgtttgcgg ccacagttgc cagagatgaa
     3301 gcccttcacc cgagactaaa cgattaggcg cgtaactgat aaacggcagc gccgctgcga
     3361 ttgccgtcag caacaacagc agcgccagaa caggattaat acgtagataa gtcacgggaa
     3421 attacttcgc ccaccctttt tgtttcaggt agtcggcagc cacttttttg gcatccagtc
     3481 cttccactgc aatgctggca ttcagttgct gcaatgtttt tgcatcgagg ctggcgaaga
     3541 ctggctgtag ccactgtgcc atttgcggat actccttcaa caccgattca cgcaccactg
     3601 gtgcaggcgc gtagataggt tgcacgcctt gcggatcgct taaggtttgc agccccagcg
     3661 ccgcgaccgg agcgtcagtg ccgtaagcca ttgcggcatt aacgccagag gtttgctggg
     3721 cagcggcttt aatcgtcacc gccgtgtcgc caccagccag tgacagcaac tgatcctgac
     3781 cgagcttaaa gccataggct ttttcaaacg cgggtaacgc atcggcgcgt tcgataaact
     3841 ctgccgaggc tgccagtttg aaggtgccgc cctcttgcag ataacgactc aggtcggcaa
     3901 gcgaagtgag tttgtttttc tctgccacat cctgacgcac ggcgatagtc caggtgttat
     3961 ttgcaggcgc gggcgtcagc cagattaact tgttgtgctc cgcatcgagc tttttgactt
     4021 tctcgtagcc ctgcccggca tttttccacg ctgcatcgtt ttcatcttta aagaagaaag
     4081 cgccattgcc ggtatattcc ggatagatat ccagttcacc ggaagtaatc gccccgcgca
     4141 ccacaggagt cgttccgagt tgcactttat tcaccgtcgg tacgccgtgg ctttcgagta
     4201 cctgcaagat gatgttgccg agcagcgcgc cttcggtatc gatttttgaa ccgactttaa
     4261 cgggggaagc cgcctatagc agcaggctca cggctgccaa cataaccagt gaacctgccc
     4321 agagctttaa gagtggcatg atgctttcct cattctttta ctgttgtttt cagcgaatta
     4381 agagaaaagc atagttgata atgacagagt tagcctgagc ggaaggattc agttgcagaa
     4441 tcagataaat acttaagagg catattcggt ccggcgtttc ctgccggatg cggcgcgagc
     4501 gccttatccg gcctacaaag ggcgaaaatt cagcaagtta agatatgaac atcaggcaaa
     4561 gggcgcaaac gtcgcgccct tactaaagca ttacagcaac tcaaactcgc ctttcttaac
     4621 gcgtgcggaa tcagtgccga taaatacatt gaacttgcca ggctcggcgt catatttcat
     4681 ctgttgattc cagaacttca gcgcctcaat atcgatcggg aagctgacag tctgagtttc
     4741 gcccggtttc agggtgattt tctcaaagcc tttcagctgt ttcacagggc gactcatgga
     4801 agccgtcaca tcctgcaagt acatctgcac taccgtggcc ccctcgcgct taccggtgtt
     4861 cgtcacctgc acgctggccg tcactttgcc gtcacgcttc atggtcggcg cagaaagttt
     4921 cacatcagag acggtgaaag tggtatagct cagaccatag ccgaacggat aaagcgcccc
     4981 gttagcttca tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacggacg
     5041 accggtgttc agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggacat
     5101 tggcagcttg ccggacgggt tgtaatcgcc aaacaataca tcggcaattg cattaccgcc
     5161 ttcagtcccc gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag
     5221 cgccagcgga cgcccgttca tcagcaccag caccagcggt ttaccggtgg ctttcagcgc
     5281 cgcaatcaag tcacgttggc cttgcggaat agtgatatcg gtccggctgg aggcttcgtg
     5341 cgccatcccc tgtgcttcac cgactacagc caccaccaca tcagattgtt tcgccgtctg
     5401 caccgcttca tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata
     5461 ctgattcagg aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag
     5521 cactttaccg ttttcaccga cggaattttt aatcccggtc agtacggtca cggattgatc
     5581 ggcaacaccg gctgcggacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc
     5641 aaccaccgca atggtggccg attttttcag cggtaacgtt tcgagacggt ttttcagcaa
     5701 cactaggctt tcgcgcgcca cttcacgcgc ttctttacgg tgcaggcggc tttcggcatt
     5761 ggtatccacc gggtcagact ctttcggccc caaatggctg tatgggtcgt taaacaaccc
     5821 catatcatat ttaacgttca gtacatggcg ggcagcatcg tccagctctg ccatggtcac
     5881 tttgccggat ttaatcaacc caggcagata cttcgagtag tactcgtcgc tcatactcat
     5941 gttgattccg gatttcagcg ccacgcgcac cgcatcttcc gggtctgccg ccgtgccatg
     6001 tttaatcagc tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg
     6061 gtcgcgcaga acatctttca gcagccagga atcggaggtg gctggcgtgc cgttcagcga
     6121 gttcagcgcc accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat
     6181 ataatcatta aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc
     6241 taccgcgccg tatgcggcaa agtgtttgac gctggtcatc accgagtaac gatctgccgg
     6301 gcttttaccc tgcatcgctt ccaccatggt tttacccatt attgaggtga gatacgtatc
     6361 ttcgccaaaa ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg
     6421 tgcccaggtc atattcaggc catcatctgc cgcttcataa gcagagacac gcccgaccgt
     6481 tttcaccgca tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg
     6541 ctgaccgtgc agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat
     6601 cacctgatcc tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tcgccccaac
     6661 ctgaccgtct ttgatcatct cgcggatcgc ctctttcgga ttatccgggc ctacgctgat
     6721 taagcgcagc tgaccaattt tctcatcaac catcattttc ttgagcagat cggtgacgaa
     6781 cgcatcccgc gcttcaggcg ttaatggatg gttaccgaat aaatcatccg ccagtgctgg
     6841 ctgcaggacc agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct
     6901 tcctttgttg cccgacgtgg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat
     6961 gatggagaga gaaaaagaat actcagttta ttgtctgaat tttcaaaata ttcactcgct
     7021 gaattgtcat acaaggcgct attctagttt gtgatatttt ttcgccacca caaggagtgg
     7081 aaaatgtctt ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg
     7141 gtcggtcatt cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc
     7201 cgttctggtc agggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg
     7261 cgggtgctga aagcctgcgt caccgccgac aaaattattc tgatgcaggc tgccaataca
     7321 ggcctgaccg aaggatcgac gccaaacggt aacgactatg atcgcgatat cgtgatcatc
     7381 agcaccctgc gtctcgacaa gctgcacgtt ctcggcaagg gcgaacaggt gctggcctat
     7441 ccgggcacca cgctctattc gctggaaaaa gccctcaaac cgctgggacg cgaaccgcat
     7501 tcggtgattg gatcatcgtg tataggcgca tcggtcatcg gcggtatttg taacaactcc
     7561 ggcggctcgc tggtgcaacg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata
     7621 gatgaagatg gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg
     7681 gagcagatcc ttagcaagct ggatgacgat cgcatcaaag atgacgatgt gcgtcacgat
     7741 ggtcgtcacg cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg
     7801 cccgcacgtt ataacgccga tccggatcgg ttatttgaat cttctggttg cgccgggaag
     7861 ctggccgtct ttgcggtacg tcttgatacc ttcgaagcgg aaaaaaatca gcaggtgttt
     7921 tatatcggca ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaac
     7981 ttcgaaaatc tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa
     8041 aaatacggca aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc
     8101 ttctttaatc tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg
     8161 cattttactg accgtgcaat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg
     8221 cgcatgaaaa actggcgcga taaatacgag catcatctgc tgttaaaaat ggcaggcgat
     8281 ggcgtcggtg aagccaaatc gtggctggtg gattatttca aacaggccga gggcgatttc
     8341 tttgtctgta cgccggagga aggtagcaaa gcgtttttac accgtttcgc cgctgcgggc
     8401 gcagcaattc gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcactggat
     8461 atcgctctgc ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc
     8521 cagctggtgc acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac
     8581 atagtgaaaa aaggcgtgga tgtgcatgcg ttaaaagaac agatgctgga actgctacag
     8641 cagcgcggcg cgcagtaccc tgccgagcat aacgtcggtc atttgtataa agcaccggag
     8701 acgttgcaga agttctatcg cgagaacgat ccgaccaata gcatgaatcc ggggatcggt
     8761 aaaaccagta aacggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat
     8821 cgccatccga attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg
     8881 ctttttgttt tttatagctc aacgctgctg ccggcacagg catcacttta ccggtttcaa
     8941 tccaggtacg caggcggctg gcatcggcaa aatgggtata tttgccaaac gcgtccatca
     9001 ctaccagcgc caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg
     9061 cattggtaaa gccggttttg gttaactgaa tattccagtt atcgcgatac accagatgat
     9121 tagtattgcg gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg
     9181 tagtacttaa ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt
     9241 cacgggcagt tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt
     9301 tcattccgag cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg
     9361 gataatggtg cgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca
     9421 gcatatcttt acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct
     9481 ccggcgtctg gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca
     9541 caaccatcgc ggtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt
     9601 tcgaatagat cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt
     9661 ccggttgtga agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gcaaaaggca
     9721 cagccagcat cagggccagg ctaaataaag aaactcgaaa tttcggcatg atgagcattc
     9781 agatagtggt tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc
     9841 gacgccatca taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag taaatgctgc
     9901 attgctgaca tttacgccag caatgcaacg tcaaagaact ttctagaaca aacgataacc
     9961 gtagccccat agtataacgg ttagggtgag cagcacttcc agtaccagca cgccaatcgc
    10021 cagcgtcgaa ctggagaagc taaggccttc ctctttgttg atattcagga agctcggaat
    10081 accaaggtaa agcaggtagc cggtgtaaaa cagcgccacc gtgccgacca gcgcacacaa
    10141 ccagaccagt ggataaagcg ccaccagacc gcttaaaaac agcggagttg caacgtagcc
    10201 cgcgaagacc atgcagtgcg caagagacgg acgctgcggg taattgcgcg ccatccacca
    10261 gatgacccgc cccatcaccg caaccccagc cagcataacg ccgtaaaaca agacagccag
    10321 cgccagtccg gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat
    10381 ctgtgtagtg ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg
    10441 ggtgtaatgg tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg
    10501 atgggaaaac agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct
    10561 gacagttcaa gtataagcca gcttgtgatt attttttgtt catccatgta atttgcaccg
    10621 tctgatgtat gtcctttttc agcgtgaatg attcacaggg tgtatgctta caggcaacca
    10681 aagggagaca gactggccta tggatctcaa tacactcatc tcacaatatg gttatgccgc
    10741 gctggttatc ggtagcctag cggaaggtga aaccgtgact ttgctgggag gcgttgcggc
    10801 acatcagggg ctattaaagt tcccactggt ggtactttct gtggcgcttg gtggcatgat
    10861 tggcgaccag gtactctatc tgtgcgggcg gcggtttggc ggcaagctgt tacgccgttt
    10921 ctcgaaacat caggataaaa ttgagcgggc gcagaaactt atccaacgcc atccgtatct
    10981 gtttgtcatt ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg
    11041 tgccagccag ctgccgccga aaatctttct gccgctgaat attctcggcg catttgcctg
    11101 ggcgttgatt tttaccacta ttggttacgc tggtggtcag gtgattgcgc cgtggttgca
    11161 caatctcgac cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt
    11221 gggcgtgcgc tggtggctga aacggcgcgg gaagaaaaag ccggataatc aggcgtaaag
    11281 cagttgccac agataagcat tcactccaca tccggcaccg ggtgttccat gcctgatgcg
    11341 acgctggtgt cttatctggc ctaacccatt gaaatatcca cgcacctcac tgcgctattc
    11401 tgggttgaac tgcggattcg ccaacataaa gccgccatcc actatcaacg actgccctgt
    11461 cgtgtaattt gcgccctccg aacaaagcca caccaccagg ctggcaatct catgcgttgc
    11521 gccaaaacgc cgtagaggaa tcgaaggctc cgcgtcgggc ttcacgtcgc tatcatccat
    11581 gctgttcatt ggcgtggcta tcgccccagg cgcgactgcg ttcaccaaaa tcttatgcct
    11641 gaccaactcc agcgccatcg ttttggttaa cccgccgagc gcatgtttag cggctgtgta
    11701 ggcgctggca tccggcagcg gcgtatgttc atgtaccgac gtaatgttga tgatgcgacc
    11761 gccctgccct tatttcacca tctgacgagc cgcaatttgc gagcataaaa atgcaccatc
    11821 aacatcaacg gtaaaaatct tgcgccactc atcaaaagcc atatcaagaa acggcgcttt
    11881 ggtcattgca cccgcattat tcaccagcac atcaatacgc ccaaatcgct ggatgagtgt
    11941 ctccagcgcc tgcgcacctt ctggcagatt gccgagatcc agttgcacga tctccgcacg
    12001 tacgccgtgg gtaaccacct cacgcgcggt atcttttgcc ccttcttcat ctgagtgcca
    12061 ggtaatgcca atatcaaacc cctgatgcgc cagtaataac gcgcactctt tgccgatccc
    12121 cgaatcggaa gcggtaatta tcgcgaccta tgccatcgag ttctccactt aacgctgaat
    12181 aaacgttaag tatagaaggc gcatatcatc agcgtttgta acccccgccc agcgcaccgg
    12241 tgagtcgaat ggaggcatcc agccactgcc cttgcaataa caggccattg gcccgctcac
    12301 gcagcgccgg gattctggct tcgctgacgc gggaaccagc aatgatgccc gcgttaaagc
    12361 gggcctgcgc aagacctacc acacgcaagg catcgcgctc aatttgcgcc tgatgctggt
    12421 ttttctccgc cagtgtctga acctgactgg ctgcccgcgc cacgtcattc actgcttcaa
    12481 ccaccgcttt gttgtagctg gcgatagaca agttgctttc ggcttttgcg atatcgagat
    12541 tggcgttaag acgaccacta tcgaaaatag gtagcgtcag gcctgccgta acgcccattt
    12601 gctgcgcgga atgacggaac agatcgctta agtgcaacgc atcctgttgc aggaaggcca
    12661 tcaggttgat gtcaggataa aatgccgctt ttgccgcatc aatagtgctt aacgatgact
    12721 caacgtacca gtgcgccgcc tgcaaatctg cccgccgggc cagtaaggag taccccagtt
    12781 catcaggaag ctggcttgcc actttcggca acgcgaccgg tttaagcttc aatgacttag
    12841 tctggtgatt tgtgagtgca tttaaccgtg cctcaataat tttcattttc cccacgacat
    12901 cgttgagctg ctgccgggtt ttgctggcat taatatcggt ttccataccg tcaactgaag
    12961 aagtaatccc gttctgatat agctggcgat cggtcgcgat aatggtgttc tgctcttttt
    13021 ctatttgctg caagaccgtg tttaacgccg cctgggtttg ccactcccag tacaggcggg
    13081 ctacgctgcc agccagcaat tggcgggttt gctcgcgttc cgccgcccgt gctttaaccg
    13141 tacccaggcg ggcagtaacc tccgcccgat tctttcccca gatatcgaga tgccagcccg
    13201 ccgttaagcc aaaagttccg ttggtgtacc acggaccggt cgtacctgcg gccggatcgt
    13261 tcagagcaaa cggccccatt agcccttctg ccgacatttt ttgccgctcc atatccgccg
    13321 aaaagtcgat ctgcggacca tcctgagtgg caactgcctt cgcctgggct tcagctagct
    13381 gaatgcgctg ttcagccacc tgcatatccg gtgcgttctg tagtgcattg ttaattaatg
    13441 aagtgagctg attatcgtga tactccagcc accattggct gtctggccaa ccatttttca
    13501 gcgccgtggg taatgcggtg tcaacttgtg cagcgggcgt ttgctggctt aacgcctggc
    13561 gggtttcatg cataggcgca cacccggcca gcatcagtaa cagcggaaaa caggcgatgg
    13621 ctggataaaa ggaatcacga ttcatggggg aataatcagg taagaaacgg tgcgcggaga
    13681 ttaccgtgtg ttgcgatata ttttttagtt tcgcgtggca atacatcagt agcaataaaa
    13741 cgacataccc agaaaaatat agactaagta aatgatatct tccgatttat cttaatcgtt
    13801 tatggataac ggcaaag
//
LOCUS       DADMGC010000109        13776 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.109, whole genome shotgun sequence.
ACCESSION   DADMGC010000109 DADMGC010000000
VERSION     DADMGC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.109"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(194..1447)
                     /locus_tag="JNZ73_003110"
                     /pseudo
     CDS             complement(194..1447)
                     /locus_tag="JNZ73_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1554..2447
                     /locus_tag="JNZ73_003111"
     CDS             1554..2447
                     /locus_tag="JNZ73_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8733306.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            2582..3802
                     /gene="mlc"
                     /locus_tag="JNZ73_003112"
                     /gene_synonym="dgsA"
     CDS             2582..3802
                     /gene="mlc"
                     /locus_tag="JNZ73_003112"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HAY8733307.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3927..4622
                     /gene="bioD"
                     /locus_tag="JNZ73_003113"
     CDS             3927..4622
                     /gene="bioD"
                     /locus_tag="JNZ73_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY8733308.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4575..5867)
                     /gene="clcB"
                     /locus_tag="JNZ73_003114"
     CDS             complement(4575..5867)
                     /gene="clcB"
                     /locus_tag="JNZ73_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HAY8733309.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6026..6640)
                     /gene="dmsD"
                     /locus_tag="JNZ73_003115"
     CDS             complement(6026..6640)
                     /gene="dmsD"
                     /locus_tag="JNZ73_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HAY8733310.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6683..7537)
                     /locus_tag="JNZ73_003116"
     CDS             complement(6683..7537)
                     /locus_tag="JNZ73_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY8733311.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7539..8156)
                     /gene="dmsB"
                     /locus_tag="JNZ73_003117"
     CDS             complement(7539..8156)
                     /gene="dmsB"
                     /locus_tag="JNZ73_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY8733312.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(8167..10437)
                     /locus_tag="JNZ73_003118"
                     /pseudo
     CDS             complement(8167..10437)
                     /locus_tag="JNZ73_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(10440..11037)
                     /locus_tag="JNZ73_003119"
                     /pseudo
     CDS             complement(10440..11037)
                     /locus_tag="JNZ73_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075019.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            11095..12695
                     /locus_tag="JNZ73_003120"
                     /pseudo
     CDS             11095..12695
                     /locus_tag="JNZ73_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397936.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            13189..13557
                     /locus_tag="JNZ73_003121"
     CDS             13189..13557
                     /locus_tag="JNZ73_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733313.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
BASE COUNT         3453 a         3512 c         3520 g         3291 t
ORIGIN      
        1 tagaataact gcgcatcagt aataaatgac acacagcaaa atgaatccgt ttatttgggt
       61 acttataatc ctgatgacgc tagatgcgct gcgggaattg gctggcgctt cgtctatttt
      121 aggatggcta ttaacgttgg tttgagcagg caataagtcc ggacgggtat ttaccgcagt
      181 ccggacttat ttttcaggcg tgcagacgac gatgcaaacg cgccccgacc agcagagcaa
      241 tgaccagcat cagagcaata aatgcgccga cgccgttcca gccatagtta tgccagaaaa
      301 caccacccag cgtcccggca atactcgacc ccagatagta actgaacaga tacagcgagg
      361 aggcctggcc tttagcgcgt tttgcgcggg ggccgatcca gctgctggct actgagtggg
      421 ctgcgaagaa tccggctgag aagagtaaca ttccggcaaa gatcagccac agcgagctaa
      481 ataaggtcat cagtaaacca aacggcataa ctcccgtcga aaacaacatc accggaccgc
      541 gcccatagcg ggtggtcatg gttccggctt tgggtgagct ccatgtaccg gtcaaataag
      601 cccgcgataa taagccaacc acggcctgac tgacatgcta gggggaaagc atcaaccgat
      661 agccgatgta attaaacagc gtgacgaacg accccatcag caaaaagcct tctgcgaaca
      721 ataacggtaa tccccggtca cgccagtgca gacgaaagtt gataaataac gtcttagggc
      781 gcagcgaagt cgggcaaaaa tggcgtgatt cagggaggat tttccagaac atcaacgccg
      841 aggccagcgc gaaacaaccg attgccgcca gagcaattcg ccagttgaaa aagtccgtga
      901 agacaccact aattaagcgt ccgctcatgc cgccaattga gttgccgctg atatacaacc
      961 ccatcaaaaa ggccacgaaa ctggggggga tttcctcgct aagataagtc atgccaacag
     1021 ctgccacgcc acttaacgaa agcccaatca aggcgcgcat aatcaaaatg ccgtgccagc
     1081 tggtcatcat tgtcgaaagt aacgtacaaa tggaggccaa cagtagcgcc gtgaccatca
     1141 ctggtttgcg accaatggca tcggatagcg ggccagtaaa tagcaaaccg atagccaaca
     1201 tcgccgtgga aatggacagt gaaatactac tgttcgcggg cgttaagcca aactcctgcg
     1261 aaagcaccgg aaggataggc tgcacacaat agagaagtgc aaaggttgcc agtccggcag
     1321 agaacagcgc cagggtgacg cgcataaatt gcggcgtacc gcgtttaata aattgatttg
     1381 gctgagaaat gctttgcttg tcagtgtcgc ttgccggagc gccatcaaca gttgtagtac
     1441 ggctcacttg aaatccttgc taaatatgcc tgtggatcag gcttatacat agggtaggaa
     1501 aatcgaattg ttctgtctaa tatattaata atctcaaata agatgtttta aatatgaata
     1561 ttgaacttcg tcatctgcgt tactttgttg ctgttgcgga agagctgcat tttgggcgcg
     1621 cggctgcccg cctgaatatt tcgcaaccgc cgctaagtca gcagattcag gcactggagc
     1681 aacaaatcgg tgcccgactg ctggcacgaa ctaatcgcag tgtattgctg acggcagcag
     1741 gaaaacagtt tcttgcagat agtcggcaaa tcctgtctat ggtggatgac gctgccgctc
     1801 gtgctgaaag actgcatcag ggtgaagcgg gggagttgcg tattggtttt acttcgtccg
     1861 ctccttttat tcgggcggtg tccgatacgt tatcgctgtt tcgccgtgat tatcctgatg
     1921 tccatttaca aacccgcgaa atgaacactc gcgagcaaat cgctccgctc attgaaggaa
     1981 cgctggatat gggattgctg cgtaacacag cgttaccgga gacgcttgaa cacgcagtca
     2041 tcgtccatga accgcttatg gcgatgatcc cgcacgatca tcccctggca aataatccga
     2101 gtgtaacact ggctgaactg gcgaaagaac cctttgtctt ttttgatccg cacgtcggga
     2161 ctgggctgta tgacgatatt ctcggactga tgcgacgtta ccatttgacg cccgtcatca
     2221 ctcaggaggt gggcgaggca atgaccatca tcggtctggt ttccgccggt ctgggtgttt
     2281 caattttgcc tgcgtcattt aaacgtgttc agctcaatga aatgcgctgg gtgccgattg
     2341 ctgaagagga tgcggtttct gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc
     2401 cagctgcgcg taactttcgt attcatctgc tgaatgctct caggtgaggg aaatttcagc
     2461 gaaaaagccc gaaaaatgtg ctgttaatca catgcctaag taaaaatttg acgacacgta
     2521 ttgaagtgct tcaccatagc ctacagatta tttcggagcg cgaaaatata gggagtatgc
     2581 ggtggttgct gaaaaccagc ctgggcacat tgatcaaata aagcagacca acgcgggcgc
     2641 ggtttatcgc ctgattgatc agcttggtcc agtctcgcgt atcgatcttt cccgtctggc
     2701 gcagctggct cctgccagta tcactaaaat tgtccgtgag atgctcgaag cacacctggt
     2761 gcgagagctg gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt
     2821 tgaaactgaa gcctggcact atctttctct gcgcattagt cgcggggaga ttttccttgc
     2881 tctgcgcgat ctgagcagca aactggtggt ggaagagtcg caggaattgg cgttaaaaga
     2941 tgacttgcca ttgctggatc gtatcatttc ccatatcgat cagtttttta tccgccacca
     3001 gaaaaaactt gagcgtctaa cttcgattgc cataaccctg ccgggaatta ttgatacgga
     3061 aaatggtatt gtacatcgca tgccgttcta cgaggatgta aaagagatgc cgctcggcga
     3121 ggcgctggag cagcataccg gcgtaccggt ttatattcag catgatatca gcgcatggac
     3181 gatggcagag gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc aggtggttat
     3241 cgatcacaac gtgggggtgg gcgtcattac cgatggtcat ctgctacacg caggcagcag
     3301 tagtctcgtg gaaataggcc acacacaggt cgacccatat ggtaaacgct gttattgcgg
     3361 gaaccacggc tgcctcgaaa ccatcgccag tgtggacagt attcttgagc tggcacagct
     3421 gcgtctcaat caatccatga gctcgatgtt acatgggcaa ccgttaaccg tggactcatt
     3481 gtgtcaggcg gcattgcgcg gcgatctact ggcaaaagac atcattatcg gggtgggcgc
     3541 gcatgtaggg cgcattcttg ccatcatggt gaatttattt aacccacaaa aaatactgat
     3601 tggctcaccg ttaagtaaag cggcagatat cctcttcccg gtcatctcag acagcatccg
     3661 tcagcaggcc cttcctgcgt atagtcagca cattagcgtt gagagtactc agttttctaa
     3721 ccagggcacg atggcaggcg ctgcactggt aaaagacgcg atgtataacg gttctttgtt
     3781 gattcgtctg ttgcagggtt aacatttttt aactgttcta ccaaaatttg cgctatctca
     3841 atttgggcca ggaaagcata acttagactt tcaaggttaa ttattttcct agtttatatt
     3901 tgtgaagcat aacggtggag ttagtgatgc tgaagcgttt ctttattacc ggtacagaca
     3961 cttctgtagg gaaaacggtg gtttcccgcg cattgctaca agcgttagcc tcccagggaa
     4021 aaacggttgc gggatataaa cccgtagcga aggggagcaa agagacaccc gaagggctgc
     4081 gtaataaaga tgccttggtg ttgcagagtg tttcaaccat cgaactgcct tatgaagcag
     4141 ttaatcctat cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt
     4201 acaccctcat ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag
     4261 aagggactgg cggctggcgc agtctgatga atgatttgcg tccactctct gaatgggtag
     4321 tgcaggaaca actgccggtg ttgatggttg tcggtattca ggaaggttgc attaaccatg
     4381 cactgctaac agctcaggcg atcgccaacg acgggctgcc gctcattggc tgggtggcta
     4441 accgaatcaa cccaggactg gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac
     4501 ttccggcacc gctcattggt gaactgcctt atctgccgcg cgccgaacag cgtgaactgg
     4561 ggcaatacat ccgcttagct atgctgcgca gtgtgctggc ggtagataga gtcacggtgt
     4621 aacgtccgcg aaattaccga cgcaattacg caggcaatca ataaaccggg gagtagctga
     4681 tactccccgg tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc
     4741 gccaacagtg tcgccatccc ggtcaatccg agtaaaagcg taatttcttc gccatcaggg
     4801 aaccataatc ccaggctacg accatacaac atgccaatgg caagaccgat aaatagcgtc
     4861 ggtgtaaaga caccaccggg tgcgccggaa ccgctactcg ccagcacggc acacagttta
     4921 caaaggaaga tcccggcaat gatcattaac agtggtgggg cggttaaaaa ggattgtacg
     4981 gtgctatagc cgttgcccca cactgcaggt gtgaacaggg aaagcagacc cacgatcaac
     5041 ccgcctagtg ccagttgcca gggcggcgca agtttgagac tcacaaatcc acgatgacag
     5101 gcgttcatta acgttaacaa cagtggtccg cacagacctg ccagcacacc tgtactgata
     5161 atcagcgcat agtcacgagc ctgaaccgtc actgagagtt gtacgttgta gagtaacgcg
     5221 tcgctatgat taatcagatt gctaaccagc aatgccacga cggcggaaat aatcaccggg
     5281 ccgagagagg ccaacatcat agtgccaaac agcacttcgg ctataaataa actgccagca
     5341 agcggggcac gataggccgc agccattccc gccgcggccc cacaggcgat ccataatttc
     5401 cattcctggc gtggcgtaaa acgttgggca aaacaggagg cggcaagggc agctaaaaga
     5461 atcatcgcac cttcgcgacc aattgcactg ccgctggtta ctaccagcag cgaggcaagc
     5521 gatttaacca ggcttgctgc gtaatcgaac tgtccatcgg tttgcaacgc ttccatgtaa
     5581 tcggtcggcg catgagggcg ttgttgggta aatttctgcc agcccatcag caacaaaccc
     5641 gccgccagtc cgccgagcgc cggagttagc agccgtcgcc agggggaaag gtttgtcgct
     5701 gcattgacca gactgccgga gtcattattg aggaacaacc actccagtag cagcatcgca
     5761 tgacgaaacc cggcaacggc aaagacagcg agaataccga cgactgttgc gataagcaga
     5821 cggcggaaca tagtgcgcag atccggatag atgtgtagat gatgcatgag cagaaatggg
     5881 caatgaaaca cgggcgacta ttttgcggca aaagaggcca accgcaaaag cagtgcgtgc
     5941 tttaagagat aaaaaaacgt gacattgtaa cgtattattg ccgaatcgcg cgtaatcgtc
     6001 accatccggc aatattacgg tgatcctatc gaaagagtgg tttaaccgcg acaggaatca
     6061 acagttgtga ttgccattgc gccagcgtta agcgagccag ttcacccagt gcacggtaga
     6121 aagggtgttc tgctttttcg ataaaaacat caagaaaacg tgttgaccac ggaaaaaggt
     6181 gccatgccag caattcttcg cattctgtct ggcgaccatt ctccgccaac cacgcagcca
     6241 tcaacagcag tgaaccaaaa tgatcttccg gttcgttttg cttcatttca aactgaatgc
     6301 ctttctcgcg catccactga cgaagtgcca atgttgaatc gccaaacagc acagattcgc
     6361 gatccagcca gaccgacccc cacggcggag acggcagtgc ccacgggccg acaaacaaac
     6421 gctgccaggc ctgggcgtga gtctcttcac actgtgtctg aaaagccgtc accagcggcg
     6481 ctaatgacgc ttctggtaga ggccactgag tttcccagcc gtcactggtg agtaccgcaa
     6541 ccagaggtgc ggcttccgca ctctctggag cgtaataaaa cagcgctccc aacacgcgcg
     6601 ccgcgacaga aaaattatct tgctgtgaaa aatgggtcat tcctaacatc ctgaaatgtg
     6661 cgggttgccc cgcaccaatt tgttaacctg caatagccat tcctactgtc atgtgcaggc
     6721 cataaaagag cacacgcccg atcatctcac caacaagtat gaggattagc cccagaacaa
     6781 gtccggcaac gtgaggttca cggcgacgaa tgagcggaca aagccagcat ccaagacctg
     6841 cgcataacaa taccacacgc cagacctgta aggaggcgta atctggtacc agtgcgctgg
     6901 cttgttgcac ggatgagtga atagacgcca gagacaaacc ttgcagcaca atcacctcgg
     6961 cacaagcgat cagcgccaga acgctaatga tggcaaatgg tgtggtatta aatgtaacgc
     7021 gggctgcccg cagaattgcg gcagcaagta ttggaccgct caacaatacc gtcaggaaga
     7081 aagccagcgt agtgtaaccg ttatgccagg ttggcacggt gtcgatttga tacacgcagg
     7141 tcatcatcca gacgaaaatg acgccgagcg ccatgctgac aagtaaccag agtttcccca
     7201 acgcttgcgg cattttaccg atgaccgcca ccagccacca tagtccgcca acggcaaaaa
     7261 atatggaacc ggcagcaatc tcgttactca gaccagaagc tccgattcga ttcagcgagt
     7321 taaacgctcg cagtggcgat cccaggtgca tgacagaggc aataaacccg atgcccatca
     7381 acagccagag gaaaaacatg ccacgaacaa tgtgctgacg gtctgcgtca tttttggcgg
     7441 cgaaccagcc aattccgcta acaatcagcg cgcctactac gcattgaccc aatacggtaa
     7501 agataaccag tggccattca tgccatccat ttcccatttt acacttcctc cggattagcc
     7561 agataaccag tggtatcgcc ggtcgggcgg ctgttggcgt taggtttgat aacgatattg
     7621 ggttttgtga aatgcgcgcg cggcagtggc gcgacggcag ccagagtacc gtgcttctgg
     7681 cgcagctctt caattggacc gaactcaagt gcacgcagcg ggcaggattc gacgcatatg
     7741 ggttgtttcc cctcggcgac gcgcgaataa caaccatcgc acttcgtcat gtgccctttt
     7801 tcagcattgt actgtggtgc gccgtacgga caagccatgt ggcagtagcg gcagccgata
     7861 caaacatctt catcaaccac cacaaagcca tcttcacgct tatgcattgc gccgctgggg
     7921 cagacttttg tacatgcggg gtcatcgcaa tggttacagg agatggagag ataataagcg
     7981 aacacgttct ggtgccagat gccattatct tcctgccagt cgccgccagc atattcataa
     8041 atacggcgga aactgacttc cgggccaaga tctttaaaat ctttgcacgc cagttcgcag
     8101 gttttacagc cagtacagcg gctggaatcg ataaaaaatc catattgtgt ggtcatgggc
     8161 tactccttaa accttttcga tctggacaag attgctgtgc gacgggtttc cctttgctag
     8221 cggtgaaggg cggtgagagg tcagaatatt gatactgccg ccgtgatcga cccggtcacc
     8281 aaacatatcc gctttaagcc acgcaccttg cccgatggcg gtaacgccag gcagaatacg
     8341 cggagtcact tttgcggcaa tcagcatttc tccattattg ttaaataccc gcacggtatc
     8401 gccatgacgg ataccgcgtg cctgagcatc aatggggttg atccacacct cttgtgggca
     8461 ggcctgctgt aacacatcaa tattgccgta gctggagtgg gtacgcgctt tgtagtggaa
     8521 gcccgttaac tgcagtggat aggttttccg caggggagcg tcccagccat caaaacctga
     8581 ggtatacgca ggaatgggat gaataatttc atcttttttc aattcccagg tatctgcaat
     8641 cttcgccagt cgttcagaat aaatttcgat tttccccgaa ggtgttttca acgggtttgc
     8701 ctgtggatct tcacggaatg cgcggaaagc gacgtagtgt tcttccgggc attttttctt
     8761 aaagatcccg gtcgttttca tctcctcgta gtcgggcatc tcagggttac gttccttcgt
     8821 tttcgcatgg agatatttga tccattcatg ctgactgcga ccttcagtaa aggtttgata
     8881 aacgtctggt cctaagcgtt tggcgacttc actcagcatc cagtagatgg gtttgcgttc
     8941 aaattttgct gaggttgcgg gttgggcgag gatcacatag cccatattcc ctgcagattc
     9001 atgagagata aggtcttctt gctctgttgg catcaggtca ggcaacagga tatcgcaata
     9061 cttagccgag gccgtcatga agtggtcaat gccaacaatc atctcgcact tgctgtcatc
     9121 ctgaagcacc tcatgggtgt gattgatgtc gccatgttga ttgatcaatg tgttactggc
     9181 gtagcaccat aaaaacttga tggggacatc cagtttttct tttccacgaa caccatcacg
     9241 ggtcgcggtc attttcgtac catggtcgat ggcatctgtc catgtaaaaa cggaaatctg
     9301 cgttttaaca ggattctcga gcatcgggaa ccattctacc cccagatccc agctaccttt
     9361 gcgtacacct gagttgccgc cgtttatgcc gacgttaccg gtaagaacgg aaagcatggc
     9421 aatagcgcgg gacgtttgtt cgccgttgga gtgtcgttgt ggcccccaac cctgacaaat
     9481 ataagcaggt tttgctgaac cgatctctcg tgccaactgg ataatttttt ctgccgggat
     9541 gctggtgatt tttgctgccc attccggcgt tttagctatg ccgtcaggcc cttcgcccag
     9601 aatataggct ttataatgcg cgttacgtgg tgcgttggcg ggcagcgttt tttcatcgta
     9661 accaacacaa tatttgtcga gaaatggctg atcgaccatg ttttcagtaa tcagtaccca
     9721 ggcaatcgca caggccagtg cgccatcggt gccagggcga atgggcagcc attcatcttc
     9781 acgccctgca gctgtgtcgt tatagcgtgg atcgatgacg atcatgcgtg cgtttgaacg
     9841 ttcgagggct tgctcgacgt agtaagtgac accaccgccg ctcatccgcg tttctgccgg
     9901 gttatttccg aacataacga ccagtttcgt attggcgata tcatccgggc tgttgccatc
     9961 attggcaccg aacatataac tcattgcggc actgatctgt gcggtactgt agctgccata
    10021 gcgactgaga aaacccccgc aagagttcat cagacggtac gggacgttcg agttggtgat
    10081 atttccgcca tctacgcctg ttccgtacag gacatgtaca gcctcattgc cgtaatcttt
    10141 caggatccgc cgaagattat cactgatggt atccagggct tcgtcccaac ttatccgttc
    10201 aaatttacct tcaccgcgct cgccgacgcg cttcatggga tatttcaacc tgtcaggatg
    10261 attcatccgt cggcggatag agcgcccgcg taaacatgcc cgaacctgat gattaccgta
    10321 aacgtcgtca cctgtcgtat cagactccac ccagtacacg gtgtcatctt tcacatgcaa
    10381 acgtagcaga cagcggctcc cgcagttaac ggtgcaggaa ctccagaccg ttttctcttc
    10441 tacccatctt tttccagttc ccaggtacgg gcaatttccg ccagcttgct ggaataaatt
    10501 tcaatcttac cggaaggcgt tttaagtgga tttgcctcgg ggtcgtcacg aaatgctttg
    10561 taggcgacaa aatggccatt gggatcttta cgcttataga tacccatttt tttcagttcg
    10621 tcgtaagacg gtaacgccgg atctttggca agcattttgg cgtacagatg ttgtaaccat
    10681 tgttcctgcg tgcgaccttc tgtgaacttt tgatagacgt caggtccaag acgtttcgcg
    10741 acttcactca ggatccagta aatcggttta cgttcgaatt tttcgctggt gactggctgg
    10801 aggaaaatga gatatcccat gttaccggcg tagtcgttag gaataatatc ttcctgctca
    10861 acggtcatca ggtctgccag cagaatgtcg gcatattttg ccgtatgagg tcataaagtt
    10921 ttcgatgacc acaatcattt cgcatttcga ttcgtcctgc agaatttcat gcgttttgtt
    10981 gatgtcagaa tgctgattaa cgagggtatt tcccgcgtag ttccagatgg actccactgt
    11041 cagacggatg gatggacttg aagtagttga ctcaacgcta cttaaggtcc tgatatgact
    11101 gacattaacc atgataatac tgtatatgca tccagttctt tctctgggtc tccctatctg
    11161 tatcagcaag tctacacctc aagtcctgac acaaggctga cacaaatcaa ggaccttgca
    11221 gtcccgcact tgctcgcaag gctcgctacg ccacagttag tccgtagagc taacggtcga
    11281 tatactatcc gcttcagact caagggacag accacaccgt ttctttcagt atcgacccgc
    11341 agcacagaca ggagagtagc cacaatgcgt cagagagagc ttgcagccac cgctaaggcc
    11401 ttcatgttgg ataggcctga agtctcacta caggagctta cagagcatct ccggtctatg
    11461 gctgagcagt tcctgacaga cgctagtgac gattactgga atggacttga ggtcgctact
    11521 ttggtggacg agaagtccaa cctgagggag ctagccgcta cgcaagcctt gagtctggac
    11581 cagcagaagg gcataaggtt agcgcttgaa gtcctgaccg cagcacagca gagggtagac
    11641 actggtgata cctctggtct gattaagctg attgatgata ataaccatac tgattactca
    11701 actataggcg actctacgtc aattctgaag aatgagcagg gggatagcca gcagttttca
    11761 cacaggagcg ccaaccatct gtagtttttt caagcctagt gtcctcgctg ctggctgaga
    11821 aggcccagac cctgaagtca tccagctaca aggacctgtc gtcctcactg aataccgtct
    11881 ctcggttctt acctgaagac atggacctga tgtcccgctc tgagtggctg gctgtcaggg
    11941 acgctatgct ggcttctgag gtgagaccgt cgacgattaa caagctgctg actaaggcga
    12001 agatgtgtct tgattacggt ctgatgaatg ggcaacttga gggccgcaac cctattgaac
    12061 ggatgaagct gaccaaggat atcgactcca agcgcagagc gttcacagac gaggagctgg
    12121 agagacttct ggtccgtgtt gaggctgagt atcagttcac acgacacacg gctcacacga
    12181 cctctgaggc ccgtagatgg gcgtctctgg tttctgtagt tactggcgct cggtctgctg
    12241 aggtctgcca cctgactaaa cgtgacattg taaccttaga caatggactg gtctgtatcg
    12301 atataaacga agatggggac ggtaagtctg tgaagaacaa gcattcagtc cgacttgtgc
    12361 cgctgaccga tggcgttcat gggttcgacc ttgagtcttt cctcaaatgg gtagacatgc
    12421 agcctgatga tggtcctctc ttcgggatga ctccgagcgc ctactctagc tggtttaact
    12481 caagggtcct gactgaggcc ctaggagact ctcaggatgt ctctctccac agtctgagac
    12541 attggctggc gacccgaatg aaagagcgtg gagtcaatct ggtggacgct caaggcatct
    12601 taggtcacag cagtcagtca attacgtatg acctgtacgg gaaaggacac gctgtgggta
    12661 ggctggctga tgtgcttaag acagctttac tttaagtagg gagacccaag gtggagacca
    12721 tatacggctt gatggttgag accttgggtc actgaagtct gactgaagtt agggagggtg
    12781 ataataatca taatccctcc tgactatagg cccactggtc cgccttcggc tgtccgactc
    12841 taagtctgtc tgtggtctta tttaaactgc gtcctaaact ccagtccctc caaattccta
    12901 tggtctcctc ttgacattcc tgtttaactg tgagtcgcct ctggtctctc tttatatata
    12961 cttccttaat tcctgaggcg tctttagtcc ttgacatttg gttcaacctg tgagtcacgt
    13021 ccggtccccc tctttatatt tacttcctta attcctaggg tcgccttaat ccgtgacatc
    13081 cagtccagcc tgtgagtcac atcctgtcgc gctactgtat gcatcaccag tgatggactt
    13141 gattatcact ataggggaac caactaattc aactacagga gatactctat ggcacgacca
    13201 atcgcaactc acgacaacac gttcaccaaa gcctacttgc agcagcactg cggggacctg
    13261 ctgtcattcg atggtcaggg cgacctgagt ggttggctgg atgatgtcct tactggtgct
    13321 ggtcgcctga acgaatcaat ggcaagcaat accaagcctg tcagcccata cttgattctg
    13381 acccaactac taacgcacga cacattaact gtaagcgctg tgcaggagtc cctgagccgt
    13441 aagcgtgttg ccttgggtga acctatggtt tcaactcgct acgctcgcta cgtctatgct
    13501 accgtcgtca gcgccagtaa atccgtccag taccacgcca gtaaagctgg ttcatgatta
    13561 cggaccctaa actatcacta tagggagacc agcctccgcc tctgagtctg atgcgtcaga
    13621 acatgcgagc atctggtcaa ccgacattaa gtatctctcc taagtaatca aggcatgtga
    13681 ccgcctctgt cagacctcgt gtatcgacct ccagctcgtt agcgttcttc agtcatctca
    13741 gttagtagct cgtctactca ctacattggc ttaagt
//
LOCUS       DADMGC010000110        13544 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.110, whole genome shotgun sequence.
ACCESSION   DADMGC010000110 DADMGC010000000
VERSION     DADMGC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.110"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(1..892)
                     /locus_tag="JNZ73_003122"
                     /pseudo
     CDS             complement(1..892)
                     /locus_tag="JNZ73_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1133..1933
                     /locus_tag="JNZ73_003123"
     CDS             1133..1933
                     /locus_tag="JNZ73_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY8733314.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2011..2781
                     /locus_tag="JNZ73_003124"
     CDS             2011..2781
                     /locus_tag="JNZ73_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY8733315.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2829..4187)
                     /gene="mltD"
                     /locus_tag="JNZ73_003125"
     CDS             complement(2829..4187)
                     /gene="mltD"
                     /locus_tag="JNZ73_003125"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HAY8733316.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4259..5014)
                     /gene="gloB"
                     /locus_tag="JNZ73_003126"
     CDS             complement(4259..5014)
                     /gene="gloB"
                     /locus_tag="JNZ73_003126"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAY8733317.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5048..5770
                     /locus_tag="JNZ73_003127"
     CDS             5048..5770
                     /locus_tag="JNZ73_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY8733318.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5767..6234)
                     /gene="rnhA"
                     /locus_tag="JNZ73_003128"
     CDS             complement(5767..6234)
                     /gene="rnhA"
                     /locus_tag="JNZ73_003128"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAY8733319.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6299..7030
                     /gene="dnaQ"
                     /locus_tag="JNZ73_003129"
     CDS             6299..7030
                     /gene="dnaQ"
                     /locus_tag="JNZ73_003129"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAY8733320.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7163..7239
                     /locus_tag="JNZ73_003130"
     tRNA            7163..7239
                     /locus_tag="JNZ73_003130"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7197..7199,aa:Asp,seq:gtc)
     gene            complement(7370..8416)
                     /locus_tag="JNZ73_003131"
     CDS             complement(7370..8416)
                     /locus_tag="JNZ73_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HAY8733321.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8427..9302)
                     /gene="tssG"
                     /locus_tag="JNZ73_003132"
     CDS             complement(8427..9302)
                     /gene="tssG"
                     /locus_tag="JNZ73_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HAY8733322.1"
                     /translation="MEKFRLGQTPALTFAPREIASVGWQEGRLHISLYSLGLWGPNGP
                     LPLHYTELARNRTESRRDPTLTRFSDLFHHRWLTQFYQAWRSAQSAGGGLDKPQHDRF
                     AFYVASLSGQDLRESADSPLPDHARLAASAHLVRESRNPDGLATTLAHYFGVPFRIQE
                     YVLHWIAVADDEITRLGMPAPSSVIGNSALIGQAVPDMQYKFRLVIGPLTLEDYRRFL
                     PGSNNLPVLTELVRAFSGYEYCWEIELQLKPHAAPPAVTGGPYQLGWTAWAGKAMHDN
                     PVTGMIFEPEHYLAH"
     gene            complement(9419..11302)
                     /gene="tssF"
                     /locus_tag="JNZ73_003133"
     CDS             complement(9419..11302)
                     /gene="tssF"
                     /locus_tag="JNZ73_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HAY8733323.1"
                     /translation="MDPRLLGYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRPLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11318..11812)
                     /gene="tssE"
                     /locus_tag="JNZ73_003134"
     CDS             complement(11318..11812)
                     /gene="tssE"
                     /locus_tag="JNZ73_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HAY8733324.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11809..12639)
                     /locus_tag="JNZ73_003135"
     CDS             complement(11809..12639)
                     /locus_tag="JNZ73_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
                     /protein_id="HAY8733325.1"
                     /translation="MTNNNELPITLSALLRDYSVVEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGDWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPGVIL
                     PPPAWMEDLLTTLACNARGEAQEADAHRSRALEAITDTSGQWNGGAFDWISDSDSRTG
                     PVLELIAGGAYIWLPFSQICSLKSPRPAHLIDLIWKPVNVTLNNGDTHSAWLFPRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(12626..13540)
                     /gene="tagK"
                     /locus_tag="JNZ73_003136"
                     /pseudo
     CDS             complement(12626..13540)
                     /gene="tagK"
                     /locus_tag="JNZ73_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3102 a         3521 c         3617 g         3304 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattatcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aaaattgttc cgctgcccgg ctaaagctgc
      841 cgctttcgac gaccgaaaca aaaatggcga gttcttccga cgtggctttc atttttgctc
      901 ctgttgcaaa atagatgaga tattttgaat ttatttgtca ttaaaccatc aggatgcgtg
      961 atatgtcatc cgatttaatg ttctcaataa tgagcaaatt tctgaccggt gtaagcactt
     1021 gcttacataa caatatacaa ttgctcgttg aaagagtgag ctaaaatccc tataacagta
     1081 gaaccctccc gagtgcggaa gggttgacgt aatagaggtt tcaaagtcaa aagtgcgaaa
     1141 aaacacctat gccatgcgct atgttgccgg acaacctgcg gaaaggatct taccgccggg
     1201 gtcttttgcg agcatcggcc aggcattacc acctggagaa ccgttaagta ccgaagagcg
     1261 tattcggatc ctggtgtgga acatatacaa acagcaacgc gctgaatggt tgtcggtatt
     1321 aaagaactac ggcaaagatg cacatctggt gttattgcag gaagcgcaga caacgccaga
     1381 gttagtacag tttgcgaccg ctaactatct tgccgccgat caggtgcccg ctttcgtgct
     1441 gccacaacat ccttccggtg taatgaccct ttcggcggcg catccagtgt attgctgccc
     1501 gttacgtgaa cgagaaccca ttttgcgtct ggcgaagtcg gcactggtga cggtctatcc
     1561 attacctgac acccgcctgt tgatggtggt taatatacac gccgtcaact tcagtctggg
     1621 cgtggatgtc tatagtaagc agttacttcc tattggtgat cagatagctc accacagcgg
     1681 cccggtcatt atggcgggag atttcaatgc ctggagccgt agaaggatga acgcgttata
     1741 tcgctttgcg cgggaaatgt cgctgcgcca ggtgcgtttt accgatgatc agcgccgtcg
     1801 ggcgtttggc cgcccgctcg attttgtttt ctaccgtggt ctgaacgtca gcgaagcttc
     1861 tgtattggtt acgcgcgctt ccgatcacaa tccgctactc gttgaattca gtcccggcaa
     1921 gcctgataaa taaggtatgt caggtctgcc acagggcaga ccaacgtttg gcgctgtcct
     1981 tttatactca aacaacaaaa ggacagcgca atgacaacac aatcccacca tgaccacgta
     2041 gaaaagcagt ttagctctca agcctgcgaa tatctgacca gtaccgtaca tgcatccgga
     2101 cgagatttac agcgcctggc ggtgcgtctg gctgattatc ctggtgcaag tgtacttgat
     2161 atgggctgcg gagcagggca tgccagcttt gttgctgcgc aaaacgtgag cgcggtggtg
     2221 gcgtatgact tatctgccca aatgctggat gtcgtggcac aagctgccga agcccggcaa
     2281 ctgaaaaata tcaccacccg ccagggatat gctgaaagtc tgccatttgc cgataacgca
     2341 tttgatattg ttatcagccg ttattctgcc catcactggc atgatgttgg tgcagcactg
     2401 cgtgaagtga accggatatt gaaacctggc ggtaggatga ttgtgatgga cgtaatgtct
     2461 ccgggtcatc cagtgcgcga catctggtta cagacggtag aagcattacg cgatacctct
     2521 cacgtacgaa actacgccag cggtgagtgg ttgaggttaa tcaatgaagc caatctgata
     2581 gttgataatt taattacaga taagttaccg ctggaatttt gttcatgggt cgcgagaatg
     2641 cgtacgccag aagcgttagt agacgctatt cgcatttacc aacagagcgc atcgacagag
     2701 gtgagaacgt attttgcctt gcagaatgac ggctctttca ccagtgatat catcatggta
     2761 gaagcacata aagcggcata aataaaaaag gcaccggggg aatcggtacc tttttattat
     2821 ctggtttgtc aggaatctgg catgttgttg tttttcacaa acaacgtcag cttgtcgcct
     2881 ggttgcagat tcgcagtgtc gctgttccag cgcatcacat ctttgatgtt cacgccgtgg
     2941 cgtttagcaa tgcttgaaag cgaatcgcct ttgcgcacac gatacgtaat gctatcgctg
     3001 ttgtttgcca gtcgctgtgc gctactgcct gcaccaatcg tcaaactttg gcctggcttc
     3061 agcttagatc cgcgcagttt gttccactgc tgcaaatctt tggtgcttac gccgagacgt
     3121 gaagcgatgc ttgaaagcgt gtcgccagag cgtacggtgt aaacacggct gttaagcggc
     3181 gtattgtcgg caaccagcgt cgactgtaca gcagcaattt cgcctgaagc gagagattca
     3241 cgcagttgat ctgcatgctt ctttggcacc atcacgtact gcggcccact tgcgcccagc
     3301 gtggagcctt tcacgccagc gttgaatgtc ttcagcttgc tgacggaaat ccccgccata
     3361 tctgcaacct tcgccatttc aaccgggcta ctcaggtgca cacgcgccag agcacggctt
     3421 tcatcggtcg ttggcagacg tacgccataa cgcttgctgt ttttgagaat atcactcaat
     3481 gccagcattt taggcacgta ctgcttcgtt tcctgcggca acggtaacga ccagaagtcc
     3541 gtggatttcc cacgcgcttt gttcgtttta attgccttca tgacccgacc ttcgccgctg
     3601 ttataagccg ctacggtcag aagccagtcg ccgtcaaaca ttttgttcag acgctgcatc
     3661 atattcagcg cggcagttgt tgaagcaaca acatcgcgac gcgcgtcata attgcgggtc
     3721 tgtttcaaac cataattgcg ccccgtgctc ggaatgatct gccagatgcc tgcggcattg
     3781 gcgccagacg ttgcgtgagg atcaaaagcg ctctccacta tgggtagtag taccagttcc
     3841 ataggcatgt tacgtttttt aacttgccct gctatccagt acatatacgg ctctgcccgt
     3901 aaagttacat cgtggagata gctcttattg cgtaaatatt tctgtttctg ttcgcgaatc
     3961 cggtcatttt ccggaattcc catctttagc tcgtcgccaa tgaaagccca caagtcacca
     4021 tctggcgcga tagacgtccc atcgtccatc catcgtgcct gacttgtaaa ctttgctgct
     4081 tccccttgac cagctgcaga aaggctctgt gcgtgctgtt gaacgttgcc ggtactctgg
     4141 caacccacga gcaggacaga ggcgagtaat atcgcttttg ccttcatgtg tgtgtcaata
     4201 gttgcttaaa agacgaccga tcataacggc gaacggagcc gatgacaaga aagttttatc
     4261 agaacctatc tttctttgac cttaaccatg caaaacgctc ttcaggttgt tgcaataatg
     4321 tttcttcatt aattacatta attaaatcaa tatcttccgt tcttaaaaaa acattaattt
     4381 gccgctcatt tttcagaatt acgggtagtg ttatttgatt ttttgcccgt aactccttaa
     4441 ctttacgata ataatcattt atggacaaat cgtgcggaaa aatactcaaa gcaaacttca
     4501 tatttgataa cgtatattca tgagcacaac ataccaatgt atcgtcaggt aacgcactta
     4561 gctttttaag tgattgatac atttgtaaag ctgtcccttc aaacaaccga ccacacccac
     4621 cagaaaacag cgtgtcgccg caaaatagat aaggtttact gaagtaacag atatgtccta
     4681 aagtgtgacc cggtgtggta attacactaa attcatgccc taaaacgaag gcagtttcgc
     4741 catctttgac tacctgtgtt gttcccttat cttgtgtctc ttgtggacca tacaccacaa
     4801 tttgcggaaa cttttccacc agttctttta cgccgccaac gtgatcgtgg tgatggtggg
     4861 ttagaaatat ggcctccggt tgccagttat tggcggtaat ggcgtttaat actggctctg
     4921 cgtctccggg atcgacaatc aggcagcgac ctgcctcatc attcaaaacc cagatgtaat
     4981 tgtcatcaaa ggcgggaata ctgttaagat tcatagatta cctctcagtg taaaacggaa
     5041 ggttgtgatg aaaccggcaa gagtccctca aactgtcgtg gctcctgatt gctggggcga
     5101 tttgccctgg ggagagcttt atcgcaaggc gctggagcgc cagctcaacc cgtggttcac
     5161 taaaatgtat ggttttcatc tgcttaagat tggcaattta agcgcagaaa tcaattgcga
     5221 agcgtgcgcg gtttctcatc aagtgaatgt ttctgcgcaa ggaatgcccg tccaggtgca
     5281 ggcggatcca cttcatcttc cttttgccga taaatccgtt gatatttgtc tactggcaca
     5341 taccttgccg tggtgcaccg atccgcatcg tttattacgt gaagccgatc gggtactgat
     5401 tgatgatggc tggctggtga ttagtggctt caaccccatc agtttaatgg gattacgcaa
     5461 acttgtgccg gtattgcgca aaacctcgcc ctataatagc cggatgttta ctctgatgcg
     5521 gcaactggac tggctctctt tgttgaattt tgaagtgctg cacgccagcc gtttccacgt
     5581 tctcccgtgg aacaaacacg gaggaaaact attgaatgcg catattcctg cgcttggttg
     5641 cttacaactt attgttgccc ggaaacggac tattccttta acgctaaatc cgatgaaaca
     5701 gagtaaaaac aagccacgaa ttcgccaggc ggttggagcc acccggcaat gtcgtaaacc
     5761 acaggcttaa acttcgactt ggtagcccgt atcttccagt gtgggattca tcgccgcggc
     5821 acgagccagt tcatcacagc gttcgttttc cgggtgtccg gcatggcctt taacccattc
     5881 ccatttgatt tgatgctgcc ccaatgcagc atcaagacgt tgccagagat cgacattttt
     5941 tactggtttt ttgtctgctg ttttccagcc acgttttttc cagttatgga tccactgggt
     6001 gataccctgg cggacatact ggctgtcggt actcaaaatg acttcgcaat gttcttttaa
     6061 cgcctccagc gcgacaatag cggccatcaa ctccatacgg ttgttggtgg tgcgggtgta
     6121 gcctgcgcta aaggttttct cgcgtccgcg atagcgtaaa atagcgccgt aacccccagg
     6181 tccaggatta cccagacacg aaccatcggt gaaaatttct acctgtttaa gcatctctgg
     6241 tagacttcct gtaattgaat cgaactgtaa aacgacaagt ctgacataaa tgaccgctat
     6301 gagcactgca attacacgcc agatcgttct cgataccgaa accaccggta tgaaccagat
     6361 tggtgcacac tatgaaggcc acaagatcat tgagattggt gctgttgaag tagtgaaccg
     6421 tcgcctgacg ggcaataact tccatgttta tctcaaaccc gatcggctgg tggatccgga
     6481 agcctttggc gtacatggta ttgctgatga atttttgctc gataagccca cgtttgccga
     6541 agtagccgat gagtttatgg actatatccg cggcgcggag ttggtgatcc ataacgcagc
     6601 gttcgatata ggctttatgg actacgagtt ttcgttgctt aagcgcgata ttccgaagac
     6661 caataccttc tgtaaggtca ccgatagcct tgcggtggcg aggaaaatgt ttcccggtaa
     6721 acgcaacagc ctcgatgcgc tatgtgcccg ctacgaaata gataacagta aacgaacgct
     6781 gcacggggca ttactcgatg cccagatcct tgcggaagtt tatctggcga tgaccggtgg
     6841 tcaaacgtcg atggcttttg cgatggaagg agagacacag cagcaacaag gtgaagcaac
     6901 aattcagcgc attgtacgtc aggcaagtaa gttacgcgtt gtttttgcga cagatgaaga
     6961 gctggcagca catgaagccc gtctcgatct ggtgcagaag aaaggcggaa gctgcctctg
     7021 gcgggcataa atacatgtaa aaggcgctaa aaatagcgac ttgggcgatt tttgcagcaa
     7081 acgattcaaa agatgagaaa aatcgttgac gaaggtcgag gcaatccgta atattcgcct
     7141 cgttcccaac ggaacacaac gcggagcggt agttcagtcg gttagaatac ctgcctgtca
     7201 cgcagggggt cgcgggttcg agtcccgtcc gttccgccaa tattcagaaa gcccgaatca
     7261 gtaatgattc gggctttcgt cgttttggtg aagtgagaat ttttcactcc gaagtaaaac
     7321 ggatatgatt taaaaaatgg ccagacatta taaattctgg ctacttctgt tactccagcg
     7381 catcccattt ctccagcagt tctactggaa tttcatttat tacctccgag acgcgttttc
     7441 cgaccaggcg ttctgcctgg cgcagcaaag gaatggtcgg gctgctcggt tcgctgtgct
     7501 caaaccagcg acggatgaca cgcaggcgtt ccagcgcgtc gtttcggtcg cggatctgcc
     7561 ggggttctgc gccggtcatc ggtgatattt cctccgcgat atgcaccggt agcgcaatct
     7621 gctctgcctg catctgttca gtatgaacga tggtggcgtt ttccggctgc tcagcctgtg
     7681 gttgtggcag gatctccggc gtggtgctct gcactgctcc cggtaacagg gccagcagtt
     7741 gcgtcagacg ggagaagtcc ggtgcctggt cattcagcgt ttcacgcgca tggcgttgca
     7801 gccgctcagt ggtctctgct gcctggcgaa aggcatccag cggcagtgtg ccgcgcgctt
     7861 ccaggtctgc cagttgctgg cgtaccgatt ctggtgccag cgcatccgcc gggcgtaatg
     7921 cagatagcga acgctccaca tcccgcacct gaaggcgcat ggcagcatta tttgacagtg
     7981 taatgccacg aatatccgcc attacacctt cgtggtccag caacgccgcc agcgcgttac
     8041 tgcgtgccac cgcagcatct tcggcggtga tatcttcagt cgcgagcagt tgaggatgga
     8101 tggcgtccgg gtacgtggag cacaacgttt ccagcagcgt gagcgcctcc gacagtcccc
     8161 tggcaccggc ctgctggata cggcagcgca gcaggacaac cagaatgcgg atatctttgc
     8221 tgcgcatcag cagacgatgg gcgtcccgct caagctccgc ccagttaatg ttttccggcg
     8281 tactgacaaa atcgccatat tgcgcttctt cacgcggtgc ggcgcgggtg aacagcagca
     8341 ggtattccgg atcgtactcc agatccgggc cgcaggggca ttccgccgaa attgggtcag
     8401 acaggaatat ttccatttaa ttatcctcag tgcgccaggt agtgttctgg ttcgaaaatc
     8461 atgcccgtca ccgggttgtc gtgcatggct ttacccgccc atgcagtcca acccagttgg
     8521 tacggaccac cggtaacagc cggaggggcg gcgtggggct ttagctgtag ctcaatttcc
     8581 cagcagtatt cataaccact aaaggcgcgg accagttctg tcaggacggg caggttattg
     8641 ctgccaggca gaaaacgtcg gtaatcctcc agcgtcagcg ggccgataac cagccggaac
     8701 ttatactgca tgtccggcac ggcctggccg atgagtgcac tattgccaat aacggaagag
     8761 ggggcaggca tccccagccg ggtgatctca tcgtccgcca cggctatcca gtgcaggaca
     8821 tactcctgta tccggaaagg tacaccgaaa tagtgcgcca gcgtggtggc cagaccgtcc
     8881 ggattgcggg actcacgcac cagatgggcc gatgccgcca gacgagcgtg atctggcaac
     8941 gggctgtcag cgctctcccg caaatcctgc ccgctgaggc tggcgacata aaaggcaaag
     9001 cggtcatgtt gcggtttatc cagaccgccc ccagcggact gggcgctgcg ccatgcctgg
     9061 taaaactgcg tcagccagcg gtgatgaaaa agatcagaaa aacgggtcag cgtcggatcg
     9121 cgtcggcttt cagtccggtt acgcgccagt tcggtgtagt gcagcggcag tggaccgttc
     9181 ggcccccaca gcccgaggct gtacagactg atgtgcaggc gcccttcctg ccagcccaca
     9241 ctggcgattt ctcgtggcgc aaaggtcagc gcgggcgttt gtccgaggcg aaatttttcc
     9301 atccgtggtt gctaggtgtt gcccgccgga acggtacaca actgcacatc cacgcggcgc
     9361 atcaggttca gaaaaccgta atgccagggg ggttcctgcg cctgagtgaa atcgttgctc
     9421 atacgcttcc ccgctgtccc gccctgacag gccaggtcat aattttgccg cgctgcatgc
     9481 tgtgcagcgt catctgcgag aaggtattaa tggaaacatg gcgggcgata tagtgttcca
     9541 gcaccaggcc gaacagatac ggactgatgc cggaaaaacc ttcttcatcc accgtcagtt
     9601 cacaactgac gccacgccca tagaccagta gtccggaacc gggcagacgg cgggtgaccg
     9661 gggtcgtttt acaaccaatc aggctgcgca cctggcgcga ttgcggactg tcatgagcag
     9721 gaataaacag gttcagcaga tctcgcaacg cctggccgcc ggtacggtga tccagatcgg
     9781 ccagcggcag ataattaaaa gataactggc ggatcaaccg ccatgccatc tcacgttcag
     9841 ccagcggggg ctgcggcgag cgtggcgggc ggataagccc tacgcccgcc accgggatgg
     9901 ctgcatccac ggttagatcg tcccggccat tgcgggcaat aagacagggc agatcgcggt
     9961 tagtgaccat ggccgtgatg gtgatgtggc gcagattttc cgggtacggc gcttcatact
    10021 ggtcaaccag cgacaggaac acttccgagc ccgtataggg ggtacgggtg ccgtaacggc
    10081 gggcgttttc tgataagcgg cggggctccc ggcgcaggga aaaataacgt ccgtgattgc
    10141 cttcatcatt attgcgggtg tggtagagcg ggcgaaaggc catcttccgt gtggtgtcgg
    10201 tttccagccc ttccacttcc tggaccgaga acacctcata atccagcgga tgggttctgt
    10261 ccaccaccag atgctgttcc gtcgtgctgt gcgtgacatc gatccgggcg gtgacgcggg
    10321 gaaacaggtt gataaccggg gtacagaaca ggctgaactg tgccgcattt gtctggtgga
    10381 tcagccagtc cggcggcagg cggttcagca ggataacaat ctccgccacg ccaccgtcaa
    10441 ttttctgtaa tccggcagac aggcctgtcg gggtgaaaaa atagaagcgt tccggacagg
    10501 caaaatattc atgcagcagg ttatgaccgt gaaacacgtt ccacgccagc ggcagcagcc
    10561 cctgatccgg ctccaggcct tcgtacataa cgggctgttg cagattcaca tccagcgcgc
    10621 cgtcaaaatg ccccggaatg ccagccaggg tcgcgatggc gctggtatgt agaagttcta
    10681 acaggtgtga ggccgtacgc tcctcgccgc agagataaaa cggcagcctg tccagtccgg
    10741 caagctgact gaacgttagt tcaccaaagg tgcgcagggt aatacgcagc gccccggcga
    10801 cgtgaatgtt ggccggcagg tagcggtgca gggcaggcat atccggtggc gcggcggtca
    10861 gtcgcgcctc ttcgatggcg agcggccaca gcgttacatc ctggctgctg cgaaactggc
    10921 aggcagtgct ttcgccttcc ggaatggcgg agaaaaaggc tgtgtcgcgc gggacggtaa
    10981 atcctttcgc cagatcgcct tcttccgtat cgggatgcaa ctgcgccacg ctcattgagg
    11041 gcgtgggtgt gacgtaattg gggctgacca cttccagcgg gcgctgggtg aaacggggga
    11101 attcagcgtc aatttttagc tgcgtgcggg cggtcaggaa gctgaaggcc tcgaccattc
    11161 gctctacata cggatcggca atatccgttc cctgcatgcc cagccgggcg gcgactttgg
    11221 gatgacgggc ggcaaattcg gctcccgttt cccgcagata gctcagttcg cggttgtagt
    11281 atcccagtag ccgtggatcc atcggtctct ctctgaatta aaaaaacgta atgcgactct
    11341 gctccatatc cagcgcgcta cgtacccgga atgcagtcgg atacggctgc gtgaggatct
    11401 cgccgcgaat ttcaaactgt aatgtgttat ggccactctg gcggcgctca tcctgaagtg
    11461 ggatgatttg cagggtggac gctttcagcc ggggttcaaa ccgggtgatg gttcgcaaaa
    11521 tggccctgcg gatatcgtcc cacttgtgct catacagaaa gctgcccgcc agtggcggca
    11581 ggccgtagtt aagtacggac gctgccgcgt acggataacg tgcagcatca atatcatctt
    11641 catggctgac ggtgttgagc agaaatgaca aatcccggcg gatgatctcc ttaatctgaa
    11701 ccgggctgac gctgataccg tggtcgcgtt tctggtttgg ggcgtcgtca cacaggcggt
    11761 caaacagcgt gggcagcagg tgattgcctg gacgctggcg ggaagcgctc acgcgtcgct
    11821 tccttcagcg gtgttaaagg tacaggtgga caaatccagc aggctgatat cgccgtgacc
    11881 ggtgagccac accctctgac ccagcgcctg taccagtgtc tctcccggac cgtcctgcca
    11941 gaccgtttcc cggcacagac gcagggagtc ggcagccttt tcagagccgc tgtagcgggg
    12001 aaacagccag gcactgtgtg tatcgccatt gttcagggtg acgttgacgg gtttccagat
    12061 caaatcgatc agatgcgcag gccgcggcga tttcagcgaa cagatttgtg aaaacggcag
    12121 ccagatatat gctccgcccg cgatcagttc gagtaccgga ccggtacggg agtcgctgtc
    12181 gctaatccag tcaaatgcgc cgccattcca ctgcccggaa gtgtctgtta tggcctcaag
    12241 cgcgcggctg cggtgagcat cagcttcctg cgcttcacca cgggcgttac aggcgagggt
    12301 ggtcagcaga tcctccatcc aggcgggcgg cgggagaata acaccgggcc gctgttcgcc
    12361 ctgaaagcag gcgtggcggt atatctcaca gcggatcagc tcgccaaata cctgcgcttc
    12421 gcgagtataa tttgcgtcca tccgggcgca tagctggatc tgttgcagtg cccgcgacca
    12481 gtcgcctgcc acgcacaata actgaaacag gctgtggcgg cgggaggcct gcgctgggtg
    12541 catcctgacc tgttgctctg ccatctgaat gccctccacc acggaataat cccgcagcag
    12601 cgccgacaag gtaatcggta gctcgttatt gtttgtcatg atatatctct ccatgttgtg
    12661 ccgggtttat ccggtaatgg ttgtcaatgc caatgatgcg atgctctttc cgtgtcagat
    12721 ctggcagtac ggtgttatgc gctgtttccg gggcgtattc cggggaaagt aaatgcagga
    12781 tatcaggggg agtcttttcg acaggccaga tagtttcatc ttcgccagtg gcatccagta
    12841 cattgagaat agcgtcaatg cttaacccac cagtgaccat atcctgcaat gtctcggctt
    12901 cggtgaccag gtcacgcaat gaatccagat cctgcgagga gggctcttcg ttaaaaggaa
    12961 aaggatcagc atggcgatta agatcttgtt cgggagataa caacgcctcg cggtattcct
    13021 gataaagtcg ggtcagggca tttccttttt ccgcaccttc tccggtggag gaggctccat
    13081 gaacaggaat gatatcgaat ggattttgtg atgtcagatg ctgctgcctg aaccaatcca
    13141 gatccagata ttcggttatt gcttccgacg tgatggcagt ctgcgatatc gtttttactg
    13201 tgctcaggtc cggagatgat ggattgccat gtacaaggca ctatgaagat aacccccatt
    13261 ctatcaggtc gccttcattc agacgcatac ggtgatgagg ttccacaatc tggtcgttta
    13321 ccgtacagca atattcggta gactgattga taatccacca tgcttcttcg tggcgggtca
    13381 gagtgagggc aatctttaca tcatcattca ccttaaaaac aggaatccag ggagaggtaa
    13441 cggtgaagat aatttgtgtt ccttcagtcg cgtaaattgt gttatcggcg atcccgtgcg
    13501 cctgtatttt ttggagtccc cacgtaatat ttggtttcat tgtg
//
LOCUS       DADMGC010000111        13438 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.111, whole genome shotgun sequence.
ACCESSION   DADMGC010000111 DADMGC010000000
VERSION     DADMGC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.111"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..352
                     /locus_tag="JNZ73_003137"
     CDS             <1..352
                     /locus_tag="JNZ73_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8733326.1"
                     /translation="FRKECPLNSGYLRALDNVLQELGRERTIAMSLPEFEQSLFMAAQ
                     PDNLLLATAPRYCQYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIV
                     WLRETIKNLYASMA"
     gene            509..1258
                     /locus_tag="JNZ73_003138"
     CDS             509..1258
                     /locus_tag="JNZ73_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733327.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1280..1846
                     /gene="yieF"
                     /locus_tag="JNZ73_003139"
     CDS             1280..1846
                     /gene="yieF"
                     /locus_tag="JNZ73_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
                     /protein_id="HAY8733328.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1900..3237)
                     /locus_tag="JNZ73_003140"
     CDS             complement(1900..3237)
                     /locus_tag="JNZ73_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY8733329.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGGIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3402..4067
                     /gene="yieH"
                     /locus_tag="JNZ73_003141"
     CDS             3402..4067
                     /gene="yieH"
                     /locus_tag="JNZ73_003141"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HAY8733330.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            4134..4607
                     /locus_tag="JNZ73_003142"
     CDS             4134..4607
                     /locus_tag="JNZ73_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HAY8733331.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            4656..5243
                     /locus_tag="JNZ73_003143"
     CDS             4656..5243
                     /locus_tag="JNZ73_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733332.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(5305..6027)
                     /locus_tag="JNZ73_003144"
     CDS             complement(5305..6027)
                     /locus_tag="JNZ73_003144"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY8733333.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPILGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNMLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(6029..7204)
                     /locus_tag="JNZ73_003145"
     CDS             complement(6029..7204)
                     /locus_tag="JNZ73_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAY8733334.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVLGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYLLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            complement(7231..8847)
                     /locus_tag="JNZ73_003146"
     CDS             complement(7231..8847)
                     /locus_tag="JNZ73_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HAY8733335.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(8933..10330)
                     /locus_tag="JNZ73_003147"
     CDS             complement(8933..10330)
                     /locus_tag="JNZ73_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAY8733336.1"
                     /translation="MTKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGK
                     MEPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEA
                     GLAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTR
                     YQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLI
                     PEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHN
                     ITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSER
                     GWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQ
                     VNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFG
                     WYAEVIKTRGLSLKK"
     gene            complement(10346..12223)
                     /gene="bglF"
                     /locus_tag="JNZ73_003148"
     CDS             complement(10346..12223)
                     /gene="bglF"
                     /locus_tag="JNZ73_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HAY8733337.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(12357..13193)
                     /gene="bglG"
                     /locus_tag="JNZ73_003149"
     CDS             complement(12357..13193)
                     /gene="bglG"
                     /locus_tag="JNZ73_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HAY8733338.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         3546 a         3374 c         3080 g         3438 t
ORIGIN      
        1 tttcaggaag gagtgtccgt taaactcagg ctacctcagg gcgctggaca atgtgttaca
       61 ggagctgggg cgcgaacgca cgattgctat gagcctgccg gaattcgagc agtcactgtt
      121 tatggcagcg caacccgaca atctgctact ggcgaccgcg ccgcgctact gtcagtacta
      181 caatcaactc catcaactgc cgttggttgc tcttcctctc ccgtttgacg aaagccagca
      241 aaaaaagctg gaagttcctt ttaccctgct gtggcataaa cggaacagcc ataatccgaa
      301 gatcgtctgg ttacgggaaa ccattaaaaa cctttacgcg tcgatggcat aaccgaatcg
      361 tatgaaacgc gacccaattt cacaataaaa tgtaaaaaag ttgtaataag cttgtctgaa
      421 tcgaactttt agccgcttta gtctgtccat cattccagta aatgattact cttgtattca
      481 taatggacca ttaagcatgg agcgaaaaat ggcgactcac tttgcccgag ggattttaac
      541 ggaaggacat ctgatttctg ttcgtctccc ctcccagtgt catcaagaag cccgaaacat
      601 tccccctcat cgtcaaagcc gttttctggc gtccagaggt ttactcgcag aactgatgtt
      661 catgctgtat ggcattggcg aattgccgga aatcgtcacc ctgccgaaag gtaaaccggt
      721 tttcagtgat aaaaatttgc cttcgttttc tatttcctat gccgggaata tggttggcgt
      781 ggcgttaaca accgaaggtg aatgtggcct cgatatggaa ctacagcgtg cgacgcgcgg
      841 gtttcatagc ccacacgcgc ccgataacca caccttttcc agcaatgaat cgctatggat
      901 cagtaaacaa aacgatccta acgaagcgcg ggcgcagctc attacgctgc gccgaagcgt
      961 gctaaaacta accggtgatg ttttgaatga cgatccgcgc gatctgcagc tgctgcccat
     1021 tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa gcgttatgcg acgcggagga
     1081 cgtgctggtg tggtccgtgg cggtcacgcc cgcgattgaa aagctcagtg tctgggagtt
     1141 agatggcaaa cacagctgga aaagcctgcc ggatattcac agccgcgcca acaatcctac
     1201 cagccggatg atgcgttttg cccagctctc taccgtgaag tctttttcgc caaattgata
     1261 gacaaccaca ggagtcatta tgtctgaaaa attgcaggtg gttacgttac tggggagcct
     1321 gcgcaaaggc tcatttaatg gcatggttgc acgtaccctg ccgaaaattg ctccggcgag
     1381 catggaagtc aatgcgttac cttccattgc cgacattccc ctgtatgacg ctgacgtaca
     1441 gcaggaagaa ggttttccag caacggttga agctctggcg gaacagatcc gtcaggctga
     1501 cggtgtggtg atcgtcacgc cggaatataa ctactcggta ccgggtgggc tgaaaaatgc
     1561 catcgactgg ctttcccgcc tgccggatca accgctggcc ggtaaaccgg tgttgattca
     1621 gaccagctca atgggcgtga ttggcggcgc gcgctgtcag tatcacctgc gccagattct
     1681 ggttttcctc gatgcaatgg tgatgaacaa gccggaattt atgggcggcg tgattcagaa
     1741 caaagttgat ccgcaaaccg gagaagtgat tgatcagggt acgctggacc acctgaccgg
     1801 gcaattgacc gcatttggtg agtttattca gcgagttaag atctaaataa aaaacccgcc
     1861 agtaatcatg catggcgggt ttttaatgcg ctatcgattt taatgagcgt cgataaatac
     1921 aatcttcagg ataaacagca gcgcaacgat gattacgcac gggctaaggt cacgcagacg
     1981 cccggtacca atcttcatca cgcagtagga gataaagccc agcgcaatac cttcggtaat
     2041 cgaaaagctg aacggcatca tcacggcggt aataaacgcc ggaacagatt cagtaagatc
     2101 ctgccagttc acgcgagcaa gacttgaggt catcaacacg ccaacgtaga tcaacgcgcc
     2161 agctgcagcg tagcctggca ccattcccgc cagcggcgac agaaagataa ccagcaggaa
     2221 cagcagacca acaaccactg ccgtcagacc ggtacgaccg ccaaccgata cgccggaaga
     2281 ggactcaata taagccgtaa cggaagaagt accgataaac gaaccggtca cggaagagat
     2341 actgtcgaca tacagcgcct gcttcatgcg cgggaatttc cccttctcat ccgccagacc
     2401 tgctttatcg gtcacgccaa tcagcgtacc ggaggagtca aacaagttga ccaacatgaa
     2461 agagaaaatc ccccctgcca gcccgaggtt aaacgacccg gctaaatcta catgacccac
     2521 aactgtcatt acgctcggcg gcgcagaaac gatgccattg tagtgcacat cacccagcat
     2581 ccagcccagc agcgtcgtca ccacgataga aaccagcacc gctgcgtgaa tgttgcgcga
     2641 ggccagaata gcaatgatga aaaagccgag gatacccaga agtacgctgt gagaagtcag
     2701 attaccgatg ctcaccagcg tttccgagtt agcgacaatc acacctgcgt ttttcagccc
     2761 catcatgccg ataaacaggc cgataccgct ggtaataccc acacgcagac ttaccggaat
     2821 gttagcaatc atccagtaac gaacgcggaa aatggtcaaa agaagcagtc cgacagcgcc
     2881 ccagaagatt gcgcccatac cgatctgcca cggcagcccc attgcctgga caaccacaaa
     2941 agcgaaaaag gcgttaagcc ccatcgcagg agccagcgcc accggaaggt ttgcaaacaa
     3001 tcccatcata atgctaccga aagcagcaat cagacaggtt gttacgaaga cggcactggt
     3061 gtccatgcca gcaacgccca gaatttgtgg gttaacaaat acgatgtaga ccatcgtcag
     3121 gaaggtggtg aacccggcaa tcacttcagt tcgtgccgta gtaccatgtt cgcgcagttt
     3181 aaaaacgcgt tccagcatcc tctgaccaga agtctgggtg gtatgttgat gactcattat
     3241 ctatttccga acaaggaggg aaaatccgtc gctatcgtat accaaaatgc gacaataggc
     3301 gcgtttgtga gagacttttt tattggattt acttatacgg caacgattgc gttgcgcaaa
     3361 tcggctctac gaaaacgtta aactgattaa aaaggaaagg catgtcccgg atagaagcgg
     3421 tatttttcga ctgcgacggt acgctggtcg acagtgaagt catttgctcc cgcgcatatg
     3481 taacgatgtt tcaggaattt ggtattacgc tcgatcctga agaggtattc aaacgtttca
     3541 aaggtgtaaa actgtacgaa attatcgata ttgtttccct tgaacatggt gttacgttag
     3601 cgaaaacaga agctgaacac gtttaccgtg cagaagtcgc tcggctgttc gattcagaac
     3661 tggaagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca atgtgtgtgg
     3721 tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat atgttgcact
     3781 acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca gacccggcgt
     3841 taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt ctggttgatg
     3901 actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc tacttctgcg
     3961 ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc catctttcgc
     4021 agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc ttcacactcc
     4081 cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt gatatgtctg
     4141 tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg ttaattggtg
     4201 ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt ttggtaataa
     4261 tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt cctgcattgt
     4321 taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa aattatcgtt
     4381 gtctggctgg tggcataggt ggcgtcgtta tcaccgagat ctattgtgca gttattgtac
     4441 atattaaggg catggcttcc tcggagttgt ttgaaaacat tctttctggt gacagtctcg
     4501 ttgtccgcat cattcctgca ttgctggcag gtgtggtgat gagcagaatc attacccgtc
     4561 tgcccggatt ggatatttca tgtcctgaaa cagactcttt aagttaagcg ggatacttta
     4621 tctttgggct actcaaaagc agacaggatg ttactatgac tcaaaatatc aggccgttac
     4681 cccaattcaa atatcatccc aagccactgg aaacaggcgc atttgaacag gataaaaccg
     4741 tagagtgcga ttgctgtgaa caacagacgt cagtttatta ctcgggtccc ttttattgcg
     4801 ttgatgaagt tgaacatctc tgtccgttgt gtattgcgga cggttctgct gctgaaaagt
     4861 ttgcaggtag ttttcaggat gatgccagca tagaaggtgt tgaatttgag tatgatgaag
     4921 aggacgaatt tgccggtatt aagaacacat atcctgatga aatgctgaaa gagttggttg
     4981 aacgcacgcc aggttatcat ggatggcaac aggaattctg gcttgcgcat tgtggcgatt
     5041 tctgtgcttt tatcggctat gtgggctgga atgatataaa agatcgcctc gatgaatttg
     5101 ccaaccttga agaagattgt gagaatttcg gtattagaaa ttctgatcta gctaaatgcc
     5161 tgcaaaaggg tggtgattgt cagggttatc tcttccgctg tctccactgc ggcaagctga
     5221 gactgtgggg tgatttttcg tagttattta aataatgaga acaggccgga gcgtaattca
     5281 tacatccggc cttatttctt cagcttaacc gaacgctaat tctgcggcag cggctttatc
     5341 agcgattacc atcagcgaag gatgcagctg caacacggaa gcaggaacat cttcggtaac
     5401 cggaccttgc agcatatttt tcagtgcctg cgctttacct gcgccactga caattatcag
     5461 caagtttttc gccgccatga tgcttttcgg ccccatcgtc acgtagctgt caggcactaa
     5521 cgaaaagtcg ccgcccaact caccatgcgc cacaatatca accatctctc ccaggatcgg
     5581 gaactccacg gtttgctcat ggaaatgggt cgtattcggc aaattgccac aaaaatgacc
     5641 atctgcacct aatcccagca ccaccaaatc caggccgcct tcacgcgcca gtttctgatc
     5701 atgctcgcgg tagttatcaa tagtgagctt ctggatattc tcttctttga tccccgcagg
     5761 ggtgaaaaac agattacgca gattggtaat cgttacgcct tctccctctt tgccgcgaaa
     5821 gggaatttca tcgaaattat agaaatagca gttatcgtac caaggcttac ctttaaccag
     5881 ggtggtgagg tattcataca tgcctttggg cgtgctaccg gcggtaattg ccaggttaac
     5941 acgacgcgtc ttcgacatat aaccaagcag atggtgtgcc gcgacacggc tcatttcctg
     6001 gtaatcttcg gtaatgatta atttcatctt aatgccttat tttaaatttc tgtacgaagg
     6061 ctgcataggc aggccgccag acatccattt catggttcag gccgggatat tcctggtaat
     6121 caaacttaat ttttttctgc tcaagctcag ttttcagccc ggcgatatcc ttgccggtta
     6181 cgacatcttt atcaccaacg accacagtaa aattacgtag ttgctggtta atagctgccg
     6241 gatcgttcag ccgggccgcg acaccttcat ccggtacggt tgtcgtggta acaccactga
     6301 atgtggccag ccagccaaag ctttccagat gattcattcc ggaaaccagc gcctggtacc
     6361 cgccttgtga aagccctgcc agcgcgcgac catcggcatc tttacggaca ctaaaacgct
     6421 tgctaatcag cgggataata tcgttcatca gttcgcgatc tgccgcttta gcattcagca
     6481 gataaaagac tttacgtctt tcctgaggca cgaaatcttc gggaataatg cccttcgcat
     6541 cggtttcggt atccgggatc accaccagca tcggtttaat tttcccttca gccagcaggt
     6601 tatccatgat ttgcgggata cgcccctgat cgatagcgga acgtccagta tcaccaaaac
     6661 cgtgatagaa atagagcact ggcaaaggct cgcccatgcc ggtgtatcct ggcggggtcc
     6721 agacatacat ctgacgttca gattgcaatg cgttggagtg gtaagttatg gcgatcaaat
     6781 caccatgcgc aacagaacgc gtatccagat aactgcctag caccagaatc atactggagt
     6841 taacctggcg ctgaggctta gtcattgcgg tgcctgtatc aatgctgcgt acaccatcaa
     6901 cattgaaaaa atactcgtac aggttgcctt tcaggatagg tgtgcgccac gaccagactc
     6961 ctgcttcgtc tttggtcatc gggtgaatat tgtccggaac cggaacaccc accactacag
     7021 agacattttt tgccccaggg gcaaagtagc gaaaggtcac gctgttatcg gcattgacct
     7081 gagtcacata ctgtttcacc gggatagtgg gtgccgggct ggctggcata tccgctgcta
     7141 tggcccactg agcagaaata ccgctggcaa tagccagcgt taaagcagca atttttatat
     7201 tcatcgtaat attcctttca ttaagttcca ttaccaccag atttcagcct gggcaccgac
     7261 agcaaactgg tcgtctttat tgtcttcgaa ggtgaagcca tccagttcgt tttccagggc
     7321 tttgatatac gtggcgtaga aacgaatttc cagacgcgag gtcaacatac tggtattgac
     7381 tttaaaggta tggaagagcg tggttttata accagactca ttaaatttat tactattcgc
     7441 atctttgttt tgctgggtga aatagcccag ttcaacacct gtctggttat attggtccca
     7501 aatataggcc gggcgaacaa ccgcacgaat agattcgaaa tcagagtgtg cgcctgtttc
     7561 gtagctataa atatcgttac cgaaggagta aacaatcgcg ttagccacaa taaaatgatc
     7621 gccgatatag gcttcgccct gcgaggtcag acgaactgct gttccgccgg tgtgatcacc
     7681 ataataacga ccattaaagg tggtaaatgg acttgcgcca gcataacggc caaagttcct
     7741 ggcgatagag ttattcgcaa ccaggaagga gaattcgttg aagccacctt tatcaaattt
     7801 ctgcgttaaa gatgtgccga acatccaggt atctttccag tcataatacc cgttattatc
     7861 ctgattatct ttttccgatg cgctttcgtt tgccgtgaca taacgaccac tcaccattaa
     7921 ggtcgcttta tcccataacg ggatatcttt atagcgtaaa tcaatagtat gggtattaat
     7981 ctgctgtttg ttttgcaggc tgcgatcgta atcatcgaca tcttcgcgaa ccagcgcgat
     8041 atcaatttta cccggaccga ctttccagtt ttccagacct acacccgctg cggcatcagt
     8101 acgctgcgtt ttccagtcca gcatctggat ttcaattttc ggcgcaccgt gtttacccac
     8161 ccagaaatca gcctctggcg caaagggcag gaaacctttg gtggtaacgt acatatcgga
     8221 gaactgcata aagttctcgc caccggcgtt atcgccaaac cagccggtag agtactgctg
     8281 accaacgtta ccatccatca tcacaacggc atcaacccgt ttgccgtttt cgttgtagac
     8341 acgttgtttt aactgcaaat caaaccagcc ggagtattcg ttaccaaagc ggcccagaga
     8401 accaatcgcc catgatttag gtgaaccatg agaggcggtc ccccaaccag aacggtaata
     8461 gccgttgtaa ctaaagccaa tttcatcttt cacaaactta ctgaaatctt tcagagtcat
     8521 ggaagaataa atcgagcctg tctcactggc atttttctct tcatttttga ccagtacagc
     8581 atcaggtttt gcggcactac tggtcgggaa cggattggcg gcataccctt gatcattcgt
     8641 acttacgcta cgattcaccg tcgctggcgt atattttttc ttctcttcat ctttatactt
     8701 tttgagttcg ctttgcgttt ctcttaacgc tttttctaat aactcaaggc gttgttccac
     8761 cgttaatgat tcagccaatg attgtgcaga aaaggctaac ggtgccatta ataagatggc
     8821 agaggtaata agatttcgtc taaacataat atccctttat ggtgcaaaga aagaattaac
     8881 gtatcgcatc cagactgttc tgaatgcgac gataattaag gtgctttatt ggttattttt
     8941 ttaatgacag cccccgcgtt ttgatcactt ctgcatacca tccgaagctt ttcttacgcg
     9001 ttcttgtgag gcttccttcg ccattatcat cacgatccac ataaataaag ccgtagcgct
     9061 tagacatttg tgaatgagat gcactgacta aatcaattgg cccccaactg gtgtacccca
     9121 taatatccac accatcggca atcgcttcat ttacctgtac caggtgatcg tttaaatagg
     9181 caattcgata atcgtcctgt atcgaaccat ccgcttcaac gctgtctttt acgcctaatc
     9241 cattctcgac aataaataac ggtttttgat aacgatccca aagcgtattt aacagaaccc
     9301 gtaatccaac cggatcaatt tgccaccccc tctctgaact tttcagatgc ggattgggga
     9361 tcatattcag tatgttgccc tgcgcatttt tattaatgct ttcgtcgtgg gaaacacaac
     9421 cagtcatgta ataactaaaa gagatgaaat ctacagtatg ttttaaatct tctgcgtcac
     9481 tttcagtcat ctcaatggtg atattgtggt cgcggaagaa acgctgcata tagccgggat
     9541 actggccacg cgcctgaaca tcaccaaaga acatccagcg ccggttctct tccatggcct
     9601 gcaacatatc ctgtggctgg caggtgaggg ggtaaaccag cccaccgaga agcatattgc
     9661 cgattttcgc ttcggggatc aggctatgat aggctttaac tgcccgcgca ctggcaacca
     9721 gttgatgatg gatcgcctga taaacttccg cctcgccact ctcttctgcc agccccacgc
     9781 ccgtgaatgg cgcgtgtaac gacatattga tttcattaaa cgtcagccat aacgctactt
     9841 tatgttggta gcgggtaaag accgtgcggg cgtaatgctc gaagtgatcg atgaccgccc
     9901 gattagccca accgccgtag tttttcacca gcccatatag catttcgtaa tgggataacg
     9961 ttaccagcgg cttgatcccc gcctgcgcca tttcatcaaa cagccgatcg taaaacgcta
    10021 accccgcttc attcggttcg gcttcgtcgc cctgagggaa aattcgcgcc caggcaatgg
    10081 aaatacgcag acaggtgaag cccatctcgg caaataacgc gatatcttcc gggtaacggt
    10141 gataaaaatc gatggcgaca tctttgatat tctctttccc caggatgcgc ggttccattt
    10201 ttcccattac gccatgaggc tgtaaatctg aggtcgtgat ccctttgcca tcttccagcc
    10261 aggcaccttc cacctgattg gcagctgttg cgccacccca aagaaatgtt tctggaaatg
    10321 ctttcgtaat taactccttt tatcgttagc gaatgatgga taacagcggt tcacctgcgc
    10381 ttatctgcgc cgtgccgtgg ggtaatacgt ctgtaaaatc atcgctatta ctgattaata
    10441 ccggcgtcgt cagatcaaat ccggcctcgc gaatagcagg gatatcaaaa gaaatcagcc
    10501 gatcgcctgt attgaccttg tcacccacgt tgacgtgagc ggaaaagaat ttgccgtcca
    10561 gttttacggt gtcgataccg acatgaatca ggatctccac accatcatct gactcaatgc
    10621 caatggcgtg taatgtggcg aacaacgaag caattcgacc cgcaaccgga gaacgcactt
    10681 caccaaccga gggcagaatg gcgatacctt tacccagcag gccactggca aacgtggtat
    10741 cagcgacgtg aatgagcggc acaatctctc ccgtcatcgg tgaacagata ccgccctgct
    10801 caggtggtgt aataacctct ggtgttttct cttgcggggc accctgcgct ggctgacgtt
    10861 tactggtgat gaaatgaagc atcaacgtac caacaaatgc gcaacctatg gcaatgacac
    10921 cgccaataac gctggcccag acggtgaaat caatccccgt tgacgggatg gtctgcatga
    10981 aggtgaaaat acttggcaaa ccaaaggagt aaactttcgt ttgcgcgtag ccaatgatgg
    11041 tggcccccaa tgctccactg atacaggcga taacaaaggg gtacttacgc ggcaggttga
    11101 cgccatatac cgctggttcg gtgataccaa acagacccgt caacgccgct gatcccgcca
    11161 ccactttttt ctgcgcatcg cgttcgcaga ggaagacgcc gagcgccgcc ccgacctgcg
    11221 ccataatggc gggcattaac agcgggatca tggtgtcgta gcccagcacg gtgaagttat
    11281 tgatacacag cggcaccagg ccccagtgca gtccgaacat gacgaagatt tgccagaagc
    11341 cgcccattac cgcgcccgca aatgcaggaa ccgcctgata aagccagaga taaccggcgg
    11401 caatcatttc gcttatccag gttgatagcg gccccaccag cagaaaggtg acgggtgtga
    11461 taaccatcag acatagcaat ggtgtgaaga aatttttgat tgccgacggt aaccacgcat
    11521 taagtcggcg ttccagaatg ctgcacaacc aggcagaaaa aataatggga ataaccgatg
    11581 acgagtaatt caacaatgtg accggaatac ccaggaaatc cagccccggc gcatccgctt
    11641 tttgcccgtt ctcgaaggca gtcagaatta atggatgcac taacgctcca ccaatcacca
    11701 tggcagtaaa tggattaccg ccgaagcgtt tgcccgcggt gtatcccagg attatcggga
    11761 agaacccaaa caaggcatca ctggcgctga ataaaattaa ataagtacca ctttgttcgg
    11821 tcgtccactg aaaagtgagc gccagagcca gcataccttt caggatcccg gttgccgcca
    11881 tcagaccgat cagaggcgta aaaatacctg aaataacata aacaaagcgg tttagcagat
    11941 tacctttctc atcattttcc ggtgcctgtt gcgctttttc gccaaggcct gccacgctgt
    12001 taaccgccag gaagacatcg gccacatggt tacctatgac cacctgaaac tgaccaccgc
    12061 tttccaccac cataataata ccgggggtct ttttcagtac ctcagcttgc gctttgcttt
    12121 catcctttaa cttaaaacgt aatcgcgttg cacaatgcat cagactcaca atgttatctg
    12181 cgcccccgac tcctgcgact atttttctgg ctaactccgt cataacttgc cctctaccgc
    12241 tttgcggcaa aactccaaaa aaaaacctga aaaaaacggc ctgacgtgaa tcaagcaatt
    12301 tttttcaggt tttgcccgct tagtgcggta acaatccttt actcagtaat aatatttcag
    12361 tgttctttgc gcacgcgctc tatatttatg gctaaaaaca taatctctgc gggtgaaatt
    12421 ttacgttgat actgcaaacc aataaaaatg gcgatccgtt ccgcacattg ccatgcttgc
    12481 gggtaatttt gttttactgc ttgttgtaat gattcatcac tatcgttaat tgaagcatgt
    12541 tcaagaatac gccaggataa aaacttcaga tgtgtaacca gtcgctgata actcaagctt
    12601 tcttcctggt aattaaggct gaactgaaat tttattaatt gcagcatttc gcgcattaac
    12661 tgcgtgacac ctgcaacatc ctccatattt ccgctcattt gggcactgac cagatgcatg
    12721 gcaataaagc ccacttcatc tttcggtaac tgcacgccca accgtttatc aataatggtt
    12781 aacgcttctt cccctagctg gaactctttc gggtaaagcc gctggatatc ccacagcaac
    12841 gggttgggca gcaacacgtt ttgctgaaag cgtttaatcg caaactggca atggtcagtt
    12901 agcgagatat aaatactgtc ctgtaatttt cccaagcgct cctgcgctaa agagataaga
    12961 cgatcacagg ttgccatcac ctcaagagga atatgactta agagttcgct taatcgcccg
    13021 ttcagttcat gactgctcaa ggcatactct ttttctattc cacttgagtt aattctttcg
    13081 ccagcgcgtt tttgaaagcc aattccgcgc cccatgacga ctttttcccg ctgttgatca
    13141 tcaataacca ccacaacatt attgttgaga attttggtga tttgcatgtt catagcaagg
    13201 acctttttta taaacaaaaa aacccgactt caccagtatt ctctggttat gtcaggtttt
    13261 gcctgcgaat gcagtaacaa tccagtcatt tattaatggt ttttataacg aacatccagg
    13321 ttcggaaatt aatttaattg cgtgcagaga aaatagcaat gcgctattga taaaaatatg
    13381 accatgctcg cagttattaa ctttgtgtaa ttttaggaat ttataaagtt atatataa
//
LOCUS       DADMGC010000112        13175 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.112, whole genome shotgun sequence.
ACCESSION   DADMGC010000112 DADMGC010000000
VERSION     DADMGC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13175
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.112"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            34..435
                     /locus_tag="JNZ73_003150"
     CDS             34..435
                     /locus_tag="JNZ73_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HAY8733339.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVSAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="JNZ73_003151"
     CDS             447..818
                     /locus_tag="JNZ73_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8733340.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMISRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..1395
                     /locus_tag="JNZ73_003152"
     CDS             805..1395
                     /locus_tag="JNZ73_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8733341.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPMSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            1392..1791
                     /locus_tag="JNZ73_003153"
                     /pseudo
     CDS             1392..1791
                     /locus_tag="JNZ73_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1802..2542
                     /locus_tag="JNZ73_003154"
     CDS             1802..2542
                     /locus_tag="JNZ73_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8733342.1"
                     /translation="MTTPNPLAKTKGAGTTLWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVPLSGLVATVKG
                     VQQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            2601..2987
                     /locus_tag="JNZ73_003155"
     CDS             2601..2987
                     /locus_tag="JNZ73_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HAY8733343.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRKAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2996..3325
                     /locus_tag="JNZ73_003156"
     CDS             2996..3325
                     /locus_tag="JNZ73_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HAY8733344.1"
                     /translation="MQFVMRLAREFRRADWRQMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            3297..6236
                     /locus_tag="JNZ73_003157"
                     /pseudo
     CDS             3297..6236
                     /locus_tag="JNZ73_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            6236..6565
                     /locus_tag="JNZ73_003158"
     CDS             6236..6565
                     /locus_tag="JNZ73_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8733345.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIREVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            6565..7263
                     /locus_tag="JNZ73_003159"
     CDS             6565..7263
                     /locus_tag="JNZ73_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAY8733346.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGAGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYDRFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            7268..8011
                     /locus_tag="JNZ73_003160"
     CDS             7268..8011
                     /locus_tag="JNZ73_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8733347.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            7948..8549
                     /locus_tag="JNZ73_003161"
                     /pseudo
     CDS             7948..8549
                     /locus_tag="JNZ73_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            8610..12089
                     /locus_tag="JNZ73_003162"
     CDS             8610..12089
                     /locus_tag="JNZ73_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HAY8733348.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLERWDTPKVVKGVSFLLRLTVAADDGSERLVSTGRTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDYAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKTLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTTNKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            12157..12756
                     /locus_tag="JNZ73_003163"
     CDS             12157..12756
                     /locus_tag="JNZ73_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAY8733349.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            12820..>13175
                     /locus_tag="JNZ73_003164"
     CDS             12820..>13175
                     /locus_tag="JNZ73_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8733350.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPWYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGA"
BASE COUNT         3091 a         3105 c         4200 g         2779 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctctgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattagtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccgatgtc cggaccgctg acacaggcat ttgaagatgc ccgcgatcgc
     1321 atcattgctg cggaaatgcc gaaacagctg gggtatgccc tgaaacaaca actgaggtta
     1381 catctgagta aatgaaccgg cacacacaaa tccgtcagag cgtgctggca cgccttcggg
     1441 aacagtgtgg agacagcgcc acgttttttg acgggcttcc ggcatttatt gatgcgcaaa
     1501 ctgcctgccg tggcggtgtg gctgagtgat gctcagtaca ccggaaaaat gacggatgga
     1561 gatgactggc aggctgttct gtatattgcc gtcttcatcc gggcacaggc accggattca
     1621 gagctggata tgtggatgga gagcaccatt ttcccggccc tgaatgatgt accggcactt
     1681 tccggactca tcgacaccct gaacccactc ggttttaact atcaacgtga taatgagatg
     1741 gctacctggg cgatggcgga aatcacgtac cagatcacgt acacgaatta aggaggtggt
     1801 aatgaccaca ccaaatccac tggcaaaaac gaaaggtgcg ggaacgacgt tatggatgta
     1861 taccggcaac ggcgacgcat ttgcgaaccc tttgtcggac actgactggc tgcgtcttgc
     1921 gatggtgaag gatctgcaac ctggcgagat gaccgctgat gcagaagatg acacttatct
     1981 cgatgatgaa gatgcagact ggaaaacgac aacccagggg cagaaatccg tcggtgatac
     2041 ttcggcgacg ctggcctggc gtccgggtga cagcgggcag aaaaaactgg ttcagttgtt
     2101 cgattccggt gaagtctgcg cgtttcgtat caaatacccc aacggtacgg ttgatgtttt
     2161 ccgtggctgg ctgagttcac tgggtaaaac cattacctca aaagaggtga tgacccgtac
     2221 tgtaaaaatc agcggtgtgg ggcgtccgta tctggcagag gagggcgctg aaaccgtggg
     2281 tgttaccgga ctgacggtgg caccggcatc tgtcagtgtc aaagcgggag caaccaccac
     2341 actgaccttt acagtaaaac ctgacggggc cagtgacaaa gcgatcagtg tgcattcgtc
     2401 agatcctcag actgcttcgg tgcccctgag cgggcttgtg gccacggtga aaggcgtgca
     2461 gcagggcagt gtcagcatcg tgggcatgac ctctgacgga gagtttgtgg cagtggctgc
     2521 ggtgaccgtc agcgcaccat aacaggacga tactcatcat tgccccggtt atccggggct
     2581 ttttgcatcc ggagaacatg atgtttctga aacaggatac gtttaattat gaaaaacagt
     2641 ccgtggtgct cagtgagctg tccgggctgc agagaattga atatctgacg tttgttcagc
     2701 agcgaaaggc aaagtttgat gcacaggagg gagaactgcc ggaggctgaa cgacagattg
     2761 cttttctgcg tatgggaatg gatatcaatg cctggctggt ttcccgctca ctgtggaatg
     2821 ctgagcagtc tcaggatgtt gagacgcttt gcgcatccat tatgacaaca tggtcgtatg
     2881 atgcgctggg cgcgggggcg gagagggttc tgtcgctgag cggtatgggg accattgaga
     2941 atgccgggga tgatgatcat gaggcgctga cgccggaaaa gtcctgacgc gggaaatgca
     3001 gtttgtcatg cggcttgccc gggagttccg gcgggcagac tggcggcaga tgctgtcgga
     3061 tatgtcggcc actgagcttg gtgagtgggg cgattatttc cggatgcaga gcttcagtga
     3121 tgtgtggatg gatgcgcagt ttgcctcgct gaaggcattg atcgtgagaa tggtgtccgg
     3181 cagcagcgat gctgcggtgg ctgatttcag ccttttaccg gaagagaacg ggataccgga
     3241 gcgaacggac gaagaactga tgcatcttgg ggaaggtatt tccggaggtg tgcgttatgg
     3301 accagatagc caacctggtc attgatttgg ggattgatgc ggcagagttt aaaaatgaaa
     3361 ttccccgtat caaaaacctt ctgaatggtg cagccagcga tgcagaacgg tcttctgccc
     3421 gtatgcagcg ttttatggag cgtcagactc aggcggcccg gcagacaatg caggcggctt
     3481 cttcggctgc aacagcagca tcagcccatg cgcagacggt ggagaagaat gcacgggctc
     3541 atgaacgcat ggcccgtgag gtggaacaaa cccgtctgcg cgtggatgcc ctgaatcaga
     3601 aaatgcgcga ggaacaggcg caggccaggg cactggcgga ggcgcaggat aaagcggctg
     3661 ccgcctttta tcgccagatt gacagtgtga aacaggccgg tgcggggctt caggaattac
     3721 agcgtattca gcagcagatc cgacaggcca gaaacagtgg cggggttggt cagcaggatt
     3781 atctggcgct gatttcggag atcacggcga aaacccgtgc cctgacgcag gcagaggaac
     3841 aggccacccg gcagaaagcg gcgtttatcc gccagcttaa agagcaggca acccgccaga
     3901 atctgtcgtc ttctgagttg cttcgcgcca gggcggctca gctgggggtg ttaatcagtc
     3961 agatggcgcg cggcaatttt ggtgcgctga ggggatccgg gataacgctg gctaaccgtg
     4021 ccggatggac aggcgcactg atgtcgccga aaggcatgat gactggcggc gttattggcg
     4081 gacttgtcgc ggcggtcctg ggtctgggta aagcctggca tgacggcgga aggagggcga
     4141 ggaatttaac cgtcagctgg cgctgacggg acattatgcc ggtgtcactg tcgggcagtt
     4201 gtggaaactc agccgggcca tatccgggaa tggtatcacg caacatgcgg cagccggtgc
     4261 gctggcacag gtggtgggca gtggggcatt tcatggaaac gatattggta tggtggcgaa
     4321 agctgccgca cagatggagc gatcggttgg ccagtcggtc agcgatacca taaatcagtt
     4381 taagcggctg aaggatgatc ctgtaaatgc cgcgaaggcg ctggacaatg cgctgcattt
     4441 tttgactgcc actcagcttg agcagatacg cgtccttggg gagcaggggc ggtccagtga
     4501 tgcggcccgg atcgccatgt ctgcgctggc agaggaaacc ggtaaacgca catctgatat
     4561 tgataataat ctcaatgcgc tgggtagtac gctgcaaacc ttgtctgact ggtggaagca
     4621 gttctgggat gcggccatga atattggtcg tgaagactcg ctggatgcgc agattgatgc
     4681 gttacaggaa aaaattcagc gcgcgaaaaa atatccgtgg acaaacgcct ccacacaggt
     4741 ggagtacgat cagcagcgtc ttaacgatct tcaggagaaa aaacgccgga aggatttgca
     4801 ggatgcaaaa gcgcaggcag aacggaatta ccaggagcaa cagaaacgcc ggaatgctga
     4861 aaatgccgcg ctgaaccgga tgaatgaaac ggaagctgca cgacatcagc gggaaattgc
     4921 gcgtattaat gccatgcagt acgccgacca ggctgtcagg gatgcggcga tacagcgtga
     4981 aaatgaacgt tacgaaaaag ccattaagaa aaatacacgg gcaacccgta atgatgaggc
     5041 cacccggtta ttgctgcagt acagtcagca gcaggcacag gtggaagggc agattgctgc
     5101 tgccagacag tcagcaggca ttgccactga aaggatgact gaagcgcata aacagcttct
     5161 ggccctgcag cagcgcatca gcgacctgga cgggaaaaag ctgacggcag atgaaaagag
     5221 tgtgctggcc cgtaagaatg agctgattca ggcgctgacg ctgctggatg tgaaacagca
     5281 ggagctgcag aaacagacag cgcttaacga cctgagaaaa aaaacggttc agctgaccag
     5341 ccagctggca gacaaagaac gtgcactgcg tgagcagcac aatctggata ttgccactgc
     5401 aggtatgggg gataagcagc ggcagcgcta ccaggcacag ttgcgcatcc ggcaggaata
     5461 ccggcaacag ttgcaacagc ttgagaatga cagtcgccag aaaggcactt acgggacgga
     5521 ggactaccgg agggctgagg aggtgctgaa ggggagcctg aagcgacaac tgaatgaaaa
     5581 caaacgctac tggcaggaac tggaagtggc gcagggcgac tggaaaaacg gtgccatgcg
     5641 ggcgtttcag aattttacgg cggatgcgga taatgcggcg ggaacggcag aacagatgtt
     5701 tacagtggca ttcagcagtg ccggtaatgc actggcgaca ttctgtacca ccggtaagct
     5761 gaattttaaa tccttcacct cttccctgtt gtcagatatg gccagaatta tggcacagat
     5821 ggccatgatg caggcggtaa agggcgtcgg ttctttattc ggcttcacga ctaatgctga
     5881 tggcggtgtt taccagtctg ctgatttgag tcgctacagt ggcacggtgg ttaaccgtcc
     5941 gacgtttttt gcttttgcaa aaggcgcggg tgtgatgggg gaagcgggac ctgaagccat
     6001 tctgccactg cgtcgcggtg ctgatggtaa gctggggggt tgtggcggat actggtggtt
     6061 caggcatggt gatgtttgcc ccgcagtaca acattgagat caataacgac ggcacgaacg
     6121 ggcagatagg tccggctgcc ctgaaggtgg tttatgacct cgggaaaaaa gcggccgcgg
     6181 actttatgca acagcaggcc cgtgatggtg gccggttaag tggagcatac cggtaatgga
     6241 gacgtttcac tggaaagtgc gcccggatat gaatgtggta tcagagccga aagtggtgac
     6301 agtgaagctg ggcgatggtt atgaacagcg tcgtgcggcg ggactgaata accagttgtc
     6361 gacttacagc gtgacgatac gtgttcgtaa aggtgaacac ccatctttaa aagcctttct
     6421 ggaacggcac ggtggcgtcc gcgcatttca gtggacgcca ccttatgact ggaaactgat
     6481 cagggaggtt tgtcgtaaat ggtcggcaag cgtgggggcg ttatgggtga ctgtaacggc
     6541 cgattttgaa caggtggtga actgatgcag gatatccggc aggaaacact gaatgaatgc
     6601 acccgtgcgg agcagtcagc ccgggtggag ctctgggaaa tcgatctgac agaggtcggt
     6661 ggtgagcgtt attttttctg taatgagcag aacgaaaaag gtgagccggt cacctggcag
     6721 gggcggcagt atcaggcata ccccattcag ggggcggggt ttgaactgaa cggcaggggc
     6781 tgtgctgccc gtccgacact gacggtctct aacctgcacg gcatggtcac cgggatggcg
     6841 gaagatctgc agagtctggt cggcggaacg gtggtccgcc gtaaggttta cgaccgtttt
     6901 ctggatgcgg tgaacttcgt caacggaaac agcgacgccg atccggaaca ggaggtgatc
     6961 agccgctggc gcatcgagca gtgcagcgaa ctgagcgcgg tcagtgcctc ttttgtgctg
     7021 gccacaccaa cggagacgga tggcgcggtt ttcccggggc gtatcatgct ggcgaatacc
     7081 tgtatgtgga cttaccgttc tgatgagtgt ggttacacgg gcagggcagt ggctgacgag
     7141 ttcgacaaac caacgacgga tatccggaag gacaaatgca gcaagtgtat gcgcgggtgt
     7201 gagttgcgca acaataccgg taatttcggc ggtttccttt ccatcaataa actttctcag
     7261 taaatccatg acacagacag aatcagcgat tctggcgcac gcccggcgat gtgcgccagc
     7321 ggagtcgtgt ggattcgtgg tgagaacgcc ggagggagac aggtatcttc ccagcgagaa
     7381 tatctccggt gagccggagg aacggttccg gatggctccg gaggactggc tgcgggcaca
     7441 aatgcagggt gagattgtgg cactggtcca cagtcatccc ggtggtctgc cctggctgag
     7501 tgaggctgac cggcggctgc aggtgcagag tgatttgccg tggtggctgg tctgccgggg
     7561 ggcgattcac aagttccgct gtgtgccgca tctcaccggg cggcgctttg agcacggggt
     7621 gacggactgt tacacgctgt tccgggatgc ttatcatctg gcggggattg agatgccgga
     7681 ttttcaccgc ggggatgact ggtggcgtca cggtcagaat ctctatctgg ataatctgga
     7741 ggccacaggg ctgtatcagg tgccgttgtc atcagcacaa ccgggcgatg tgctgctgtg
     7801 ctgttttggt tcatcggtgc cgaatcatgc cgccatttac tgcggcgatg gtgagctgct
     7861 gcaccatatt cctgaacaac tgagtaaacg agagaggtat accgacaaat ggcagcgacg
     7921 cacacactcc ctctggcgtc accgggaatg gcacgcatct gcctttacgg ggatttgcaa
     7981 cgatttggcc gccgcatcga ccttcgtgtg aaaacggggg ctgaagccat ccgcgcactg
     8041 gccacacagc tcccggcgtt tcgtcagaaa ctgagtgacg gctggtatca ggtgcgcatt
     8101 gccgggcgtg atgcaggtga aaccgaattg tctgcccgtc ttaatgagcc gctggcaaat
     8161 ggtgcagtga tccacatagt accgcgtctg gtgggagcta aaagtggcgg tgtgtttcag
     8221 gcggtgctgg gggcagctgt tatggcggtt gctatatgga tgccggggta ggaattatgg
     8281 cgagtaatct gctgttttct ctcggtgcca gtatgacgct tggcggtgtt gcacagatgc
     8341 tggcaccgaa agccagaact ccccgtacac agacaacgga taacggcaaa cagaacacct
     8401 gtttctcctc actggataac atggttgccc agggcaatgt tctgcctgtt ctgtacggtg
     8461 aaatgcgcgt ggggtcgcgg gtggtatctc aggagatcag cacggcagac gaaggggatg
     8521 gtggtcaggt tgtggtgatt ggtcgctgat gcaaaatgtt tcatgtgaaa ccgcctgcgg
     8581 gcggttttgt cgtttatgga gcgtgaggaa tgggtaaagg cagcagtaag gggcataccc
     8641 cgcgcgaagc gaaggacaac ctgaaatcca cgcagttgct gagtgtgatt gatgccatca
     8701 gcgaagggcc ggttgacggt ccggtggatg gattaaaaag cgtgctgctg aacggtacgc
     8761 cggtcctgga cagcgagggg aagacaaact tttccggtgt tacggtggtg ttccgcgccg
     8821 gcgagcagga gcagacaccg ccggaggggt ttgaatcttc cggctcagag actgtgctgg
     8881 gtacggaagt gaaatacgac acgccgatca cccggaccat cacgtcggca aacattgatc
     8941 gtctgcgcct gaccttcggt gtgcaggcac tggtggaaac cacctcaaag ggggaccgga
     9001 atccgtcgga agtccgcctg ctggttcaga tacagcgtaa cggtggctgg gtgacggaaa
     9061 aagacatcac cattaagggc aaaaccactt cgcagtatct ggcctcggtg gtggtgggta
     9121 acctgccgcc gcgcccgttt aatatccgga tgcgcaggat gacaccggac agcaccacag
     9181 accagctgca gaacaaaacg ctctggtcgt cgtacaccga aatcatcgat gtgaaacagt
     9241 gctacccgaa cacggcgctg gtcggcgtgc aggtggattc agagcagttc ggtaaccagc
     9301 aggtgagtcg caattatcat cttcgcgggc gcattctgca ggtgccgtcg aactataacc
     9361 cgcagacgcg gcaatacagc ggtatctggg acggaacgct taagccagca tacagcaaca
     9421 acatggcctg gtgtctgtgg gacatgctca ctcatccgcg ctacggcatg gggaaacgtc
     9481 ttggtgcggc agatgtggac aaatgggcgc tgtatgtcat cggccagtac tgcgaccagt
     9541 cagtgccgga cggatttggc ggcacggagc cgcgcatcac ctgtaacgct tacctgacca
     9601 cacagcgtaa ggcgtgggat gttctcagtg atttctgctc ggcgatgcgc tgtatgccgg
     9661 tatggaacgg gcagacgctg acgttcgtgc aggaccgacc gtcggataaa gtgtggacct
     9721 ataaccgcag taatgtggtg atgccggatg atggcgcgcc gttccgctac agcttcagcg
     9781 ccctgaagga ccgccataat gtcgttgagg tgaactggat tgatccggac aacggtcatg
     9841 agacggcgac agagcttgtg gaggacacgc aggccattgt ccgttacggt cgtaacgtca
     9901 cgaagatgga tgcctttggc tgtaccagcc ggggacaggc gcaccgcgcc gggctgtggc
     9961 tgattaaaac ggaactgctg gaaacgcaga ccgtggactt cagcgtcggc gcagaagggc
    10021 ttcgccatgt accgggtgat gtcattgaaa tctgcgatga tgattatgcc ggtatcagca
    10081 ccggtggtcg cgtactggcg gtgaacagcc agacccggac gctgacgctc gaccgtgaaa
    10141 tcacgctgcc gtcctccggt accacgctga taagcctggt tgacggaaat ggcaatccgg
    10201 tcagcgtgga ggttcagtcc gtcaccgacg gcgtgaaggt gaaagtgagc cgggttcctg
    10261 acggcgttgc agaatacagc gtgtgggggc tgaagctgcc gacgctgcgc cagcgcctgt
    10321 tccgctgcgt gagtatccgt gagaacgacg acggcacgta tgccatcacc gccgtgcagc
    10381 atgtgccgga aaaagaggcc atcgtggata acggggcaca ctttgacggt gaccggcgcg
    10441 gcacggtgaa tggtgtcacg ccgccagcgg tgcagcatct gaccgccgaa gtcaccgcag
    10501 acagcgggga atatcaggtg ctggagcgat gggacacgcc gaaggtggtg aagggtgtga
    10561 gcttcctgct tcgcctgacc gtggcagcgg acgacggcag tgagcggctg gtcagcacag
    10621 gcaggacgac ggaaaccaca taccgcttca ggcagctggc gctggggaat tacagtctga
    10681 cagtccgggc ggtaaatgcc cgggggcagc agggcgatcc ggcgtcggta tcgttccgga
    10741 ttgcggcacc ggcagcgcct gtcactattg aactgatacc ggggtatttt cagataacgg
    10801 tggtcccgaa actggctgta tatgacccga cggtgcagtt tgagttctgg ttttcggaaa
    10861 agcggattgc ggatatcagg caggttgaaa ccagcgcccg ctatcttggc acggcgctgt
    10921 actggatagc cgccagtatc aatatcaggc cgggccatga ttattatttt tacgttcgca
    10981 gtgtgaacac cgttggcaaa tcggcatttg tggaggccgt cggtcgggcg agcgattatg
    11041 cggaaggtta tctgagtttt tataaagggt tgatcaataa aacgcatctc ggcaaggagt
    11101 tgtggacgca gattgataac ggtcagcttg cgccggacct gactgaaatc aggacgtcca
    11161 ttacgaatgt cagcaatgaa atcacgcaaa ccgtcaataa aacactggaa aatcagagtg
    11221 ctgcaatcca gcagatacag aaagttcagg ttgatacaaa taataacctg aacagcatgt
    11281 gggctgtgaa gctgcaacag atgaaggacg gacgccttta tattgcgggt atcggtgccg
    11341 gtattgagaa tacgccagca ggaatgcaga gtcaggtgct gctggcggca gacaggattg
    11401 cgatgattaa tcctgcgaat ggcaacacaa agccgatgtt tgttggtcag ggcgatcaga
    11461 tattcatgaa cgacgtgttc ctgaaacgcc tgacggctcc gaccattacc agcggcggta
    11521 atcctccggc attttccctg acacctggcg gacggctgac ggcgaaaaat gccgatatca
    11581 gcggtaacgt gaacgcgaac tccgggacgc tcaacaacgt cacgactaat aagaactgtc
    11641 gggttctggg aaaattgtcc gcgaaccaga ttgaaggcga tctcgttaaa acagtgggca
    11701 aacctttccc acgggactcc cgggcaccgg agaggtggcc atcagggacc attaccgtca
    11761 gggtttatga cgatcagccg tttgatcggc aaattgttat tcccgcggtg gcgtttcgcg
    11821 gtgctaaaca tgagcggaag aataacaata tttattcgtc atgccgcctg atagtgaaga
    11881 aaaacggtgc tgaaatttat aaccgaacga ccctggataa tacgctgata tatacgggtg
    11941 ttattgatat gcctgccggt cacggtcaca tgacgctgga gttttctgta tcggcatggc
    12001 tggtaaatgg ctggtatccc acagcaagta tcagcgattt gctggttgtt gtgatgaaga
    12061 aagccactgc aggcatcacg attagctgaa ttttataacc cagatacggg caccagaaat
    12121 ggtgcctttt ttattgcaga aaagcgagag gtaattatgc gtaaagtttg tgcagccatt
    12181 ttgtccgcag ccatctgtct ggctgtatcc ggtgcgcctg catgggcgtc tgaacatcag
    12241 tccacgctga gcgccgggta tcttcagccc catactgata tgccaggcag cgatgacctg
    12301 aagggcatta acgtgaaata ccgttatgag tttacggaca cgctggggct ggtgacgtca
    12361 ttcagctatg ccaacgctga agatgagcaa aaaacgcatt acagcgatac ccgctggcat
    12421 gaggattccg tgcgtaaccg ctggttcagc gtgatggcgg ggccgtctgt gcgcgtgaat
    12481 gaatggttca gcgcgtatgc gatggcgggc gtggcttaca gccgtgtgtc gaccttctcc
    12541 ggggattatc tccgcgtaac tgacaacaag gggaaaacgc acgatgtgct gaccgggagt
    12601 gatgacaacc gccacagcaa cacgtctctg gcgtgggggg ctggtgtgca gtttaacccg
    12661 accgaatccg tggccattga ccttgcttat gaaggttccg gcagtggcga ctggcgcact
    12721 gacggtttca ttgtgggtgt cggttataag ttctgattag ccaggtaaca cagtgttatg
    12781 acagcccgct ggttcaggcg ggctttttgt ggggtgaata tggcagtaaa gatttcaggt
    12841 gtactgaaag atggggcggg taaacctgtc gtaaattgtg cgattgaact gcgggccaga
    12901 agaaccagtc cgaccgttgt ggcacacgtt gttgccactt gcgtgacgga caataacggt
    12961 gcttatgtga ttgaggctga gccgtggtat tacgaggttg cgcttcactg taacggctgg
    13021 cagccaaccc gtgtcgggga tattgatgtg gcaccgactg atgcaccggg gacactgaac
    13081 gcgtttctga atgcaccaaa ggatggtgat ttacgtccgg aggtgatgaa gcgctttgag
    13141 gaaatggtgg cgcaggcgca gcagagtgcc ggggc
//
LOCUS       DADMGC010000113        12876 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.113, whole genome shotgun sequence.
ACCESSION   DADMGC010000113 DADMGC010000000
VERSION     DADMGC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.113"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(506..1597)
                     /gene="ychF"
                     /locus_tag="JNZ73_003165"
     CDS             complement(506..1597)
                     /gene="ychF"
                     /locus_tag="JNZ73_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAY8733351.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1714..2298)
                     /gene="pth"
                     /locus_tag="JNZ73_003166"
     CDS             complement(1714..2298)
                     /gene="pth"
                     /locus_tag="JNZ73_003166"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY8733352.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2576..2854
                     /gene="ychH"
                     /locus_tag="JNZ73_003167"
     CDS             2576..2854
                     /gene="ychH"
                     /locus_tag="JNZ73_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HAY8733353.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2909..4588)
                     /gene="dauA"
                     /locus_tag="JNZ73_003168"
     CDS             complement(2909..4588)
                     /gene="dauA"
                     /locus_tag="JNZ73_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HAY8733354.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4713..5660)
                     /locus_tag="JNZ73_003169"
     CDS             complement(4713..5660)
                     /locus_tag="JNZ73_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAY8733355.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5811..6662)
                     /gene="ispE"
                     /locus_tag="JNZ73_003170"
     CDS             complement(5811..6662)
                     /gene="ispE"
                     /locus_tag="JNZ73_003170"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAY8733356.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLKYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6662..7285)
                     /gene="lolB"
                     /locus_tag="JNZ73_003171"
     CDS             complement(6662..7285)
                     /gene="lolB"
                     /locus_tag="JNZ73_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HAY8733357.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPTNMELTDGGQRIKLKMDNWIVK"
     gene            7499..8755
                     /gene="hemA"
                     /locus_tag="JNZ73_003172"
     CDS             7499..8755
                     /gene="hemA"
                     /locus_tag="JNZ73_003172"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HAY8733358.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKNLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8797..9879
                     /gene="prfA"
                     /locus_tag="JNZ73_003173"
     CDS             8797..9879
                     /gene="prfA"
                     /locus_tag="JNZ73_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAY8733359.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9879..10712
                     /gene="prmC"
                     /locus_tag="JNZ73_003174"
     CDS             9879..10712
                     /gene="prmC"
                     /locus_tag="JNZ73_003174"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY8733360.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10709..11101
                     /locus_tag="JNZ73_003175"
     CDS             10709..11101
                     /locus_tag="JNZ73_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HAY8733361.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11105..11914
                     /locus_tag="JNZ73_003176"
     CDS             11105..11914
                     /locus_tag="JNZ73_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HAY8733362.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11950..12804
                     /gene="kdsA"
                     /locus_tag="JNZ73_003177"
     CDS             11950..12804
                     /gene="kdsA"
                     /locus_tag="JNZ73_003177"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HAY8733363.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3226 a         3231 c         3331 g         3088 t
ORIGIN      
        1 cgcttgatgt atctgcataa caaaaacaat atgcgccatt tttgcattac taataagaaa
       61 aagcaaaaaa catcgatatt catttttggt caatggattt tctggcctat cgttattatt
      121 gtgctactgg ttatcatttt catcttgcca ggatcgcacg taatcacgtt attcggggac
      181 tttatctgcg ggatattacc cttcaggtaa gtccttttct cgccagcata ttggagcgag
      241 atcaatattg atcagctgtg aagagggctg atgtgtagtg gaacaaaaac tgacgttata
      301 tcactgctaa tgagatatca gtcatctgaa cacttatgtc tttaattatt ctcgtggttc
      361 actgtaggca ataagcacaa aagtgtagga tgttacaaga atgattagga ctcggtgaaa
      421 tgaaaaatcc acgcaattgc gtggatttta tatacttttg cgctcttcat gagatttagc
      481 gaaacctcat gagacaataa attaattaga cgttgaaaag gaagttcatc acatcgccat
      541 ctttcacgat gtaatcttta ccttctgcac gcattttgcc tgcttctttc gcgccttgtt
      601 cacctttgta agtgatgaaa tcttcaaacg agatggtttg tgcacggata aagccttttt
      661 caaaatcagt atggattttg cccgctgcct gcggcgcggt tgctccaacc ggaatggtcc
      721 atgcacgcac ttctttcacc ccagcggtga agtaagtttg caggttcagc agcttataac
      781 cggcacggat cacacggttc aggcccggtt cttccagccc aagctcctgc ataaactcgt
      841 cacgttcttc gtcgtccagt tcggcaatgt ctgcttcaac agcagcacaa accggaacca
      901 caacagaacc ttctttcgcc gcgatttcac gcacctggtc aagatatggg ttgttttcaa
      961 aaccgtcttc gttgacgttg gctatgtaca ttgttggttt cagcgtcagg aagctcaggt
     1021 aacgaatagc cgctttctct tcagcgctta aatccagcgc gcgcagcata cctgcgtttt
     1081 ccagctgggg caggcatttt tccaggaccg ccagctcagc tttcgcgtct ttatcgccac
     1141 ctttggcttt cttctgtacg cgatgaatcg cacgttcgca ggtgtcgagg tctgccagcg
     1201 ccagttcggt gttgataact tcaatatcgt cagccgggtt aactttgccg gaaacgtgaa
     1261 tgatgttgtc attttcaaag cagcgaacaa cgtgaccgat cgcttcggtt tcacggatgt
     1321 tggtcaggaa ctggttaccc agaccttcgc ctttcgatgc gccttttacc agaccggcga
     1381 tatcgacaaa ttccatggtc gtgggaagcg tacgttgcgg ttttacgatt tcagccagtt
     1441 gatccaggcg agggtcaggc attggtacga cgcctgtgtt cggctcaatg gtgcagaatg
     1501 gaaagttggc cgcttcaata ccggctttgg tcagcgcgtt gaacagggta gatttcccga
     1561 cgttgggcaa accgacgata ccgcatttga atcccatgat ttaaatcacc ttaatatctt
     1621 aataatcaac ctgttattgc taacagattg cagaaatgga aataactttg cctattatac
     1681 acggcactcg gcaaaaatgc cgcagacaac gacttattgc gctttaaagg cgtgcaatcg
     1741 gttcgttgct ttggtcaagc catctgtaaa ccacatttca gtacaacgcg ccgcttcgtc
     1801 gatggcttca tcaattaact tctgttcact aacaggcggt ttgcctaaca caaaaccgac
     1861 aactttattt ttatcgcccg gatgaccgat tccgatgcgt aaacggtgaa agttagggtt
     1921 attacccaat ttactgatga tgtctttcag tccattgtga ccaccatggc caccgcccaa
     1981 cttaaatttg gcgacgccag gaggcagatc cagttcgtcg tgggccacca gaatttcgtc
     2041 cggattaatg cggaaaaaac tggccatcgc cgcaacggct ttgccgctga gattcataaa
     2101 tgtagtcggg actaacaggc ggacatcttc gcctccaagg gtgactcgcg aagtataacc
     2161 aaagaattta gcctcttcgc gcagcggagc gcgcaaacgc tctgccagta agtcaacgaa
     2221 ccaggcacca gcattatgtc gcgttgcggc gtattcagca ccggggttcg ccaggccgac
     2281 aatcaattta atcgtcacgt ttttttgtcc tgagtgtgta cataactggc gcgtagttta
     2341 ctggttgcgg ccccgcttga caaaaaactg cgtatcaaat gcagataacg taataattgc
     2401 ctgagtggac tattagaaag tcaaggtgtt caggcgttta tttgtaaagt tttgttgaaa
     2461 taagggttgt aattgtgatt acgcccgcac ataacccaca gggtgttgtc tatactttac
     2521 acataaggaa gaggggtatt ccctgttaca acccagaaag ttccggaggt gacatatgaa
     2581 acgcaaaaac gcttcgatac tcggtaacgt gctcatgggg ttgggtctgg tggtaatggt
     2641 ggtcggcgtg gggtattcaa tcctcaacca gttaccacag tttaatatgc cccagtattt
     2701 cgcacatggt gcagtgctaa gtattttcgt cggtgccatt ctctggctgg cgggtgcccg
     2761 tgttggcggg catgaacagg tgtgcgaccg ttactggtgg gttcgtcact atgacaaacg
     2821 ttgccgccgt agcgataatc gccgtcatag ctaacaaata atgcagtttg ctgaccagtc
     2881 agtttgcgct gactggtcaa tctcgtactt ataaatccgc catcgccgcg cgacgattcg
     2941 ggaagaacgc gaggcgtccc gggatcggtt gaatgcctgc gcgagccata gttcgcagtg
     3001 gctggaattc cacgttgcac acgcgcagtt cacatccttc gggcagacgc ttcacaaaac
     3061 gctggaacgc atcaagacca ccagcatcaa gtaccggaac ggcatcccac ttcagaatca
     3121 caatccgttt gccttcaaga cgtgactcca ggtccgtgaa taagccttca gcagcagcaa
     3181 aaaacagcgg gccaataacg cgcagaacca ggacatcgtc tggaacatct acgactaccg
     3241 gtgccaggcg agtcatacgt gcgatacgac gcataaacag cagcgatgcc agcacgatcc
     3301 ccacgctgat ggcaataacc atatcaaaca gcacggtcag tgacatacac agcagcatga
     3361 cgatgatgtc atctttcggc gcatgacgca gcaagtcgac cactttgtgc gcttcactca
     3421 tgttccacgc caccatcaac agcagggctg ccatggcgga aagcggcagc caggagagca
     3481 gtggtgccag taccagcagg gcgagaataa ccagaataga gtggatcacc gccgagatag
     3541 gggaagttgc cccggcacgg acgttagcgg cagaacgcgc gatggcagca gtagcggtaa
     3601 taccaccaaa gaatggagcg atgatattcc ccagcccctg accgaccagt tcactattcg
     3661 ccttgtgctt agtcccggtc ataccatcca gtaccacagc gcagagcaga gattcgatcg
     3721 ccccgagcat tgccattgag aatgccgcag gcagcagggt gcgaatagaa tcccaggtta
     3781 gcgtgaattc tgaattaggc agatcccacg gcagcaccag ttgcggcagc agttgcggaa
     3841 taccgttacc ctgagaacca tcggccagga cgtagtggaa ttgcgaaccg atggtagcaa
     3901 catgtccgcc gagcaggtta acaatcccca tcaccgcgca accagccagc aaggccggaa
     3961 ggtgaccggg taaacgaatg cccagacgcg gccagaaaac cagaatgcca agcgtcacaa
     4021 tgccaatggc agcatcaccc acattaatgg tcggcagcgc cataaataat gcgccgactt
     4081 tttgtagata atgttccggg acatgggcca tttgcagacc gagaaaatct ttaatctgca
     4141 tggtaccgat ggtgatcccg atacccgagg tgaaacctaa ggtgacggaa accggaatat
     4201 actcaatcag acgaccaaag cgtgccagcc ccatcagaat caaaaagatt cccgacaaca
     4261 aggtcgcaac cagcagtcct gccagtccaa actgttgcga aacgggatag agaattacca
     4321 caaatgccgc agtcggaccg gaaacgctaa agcgtgaccc acccgttaga gcaatgacaa
     4381 tccccgcaac agctgcggta tataaaccgt actggggtgc cacaccacta ccaatagcca
     4441 acgccatcgc cagcgggata gcaataatcc cgacggttat cccggcaatc aggtcacggg
     4501 taaaccgtgc ggcagtatat ttttctttcc agcaagcgtc gatcagagcg cggaaaggca
     4561 tcacatgtga ggaaaatatt ttgttcacaa taatgtttca tccgtgagcg catcatctgt
     4621 caactaaatg gcaggtgaag gaggcatagg tcatacaaat ggatattaca gacaaaaaaa
     4681 cccgccgcag cgggtctttg agccgggttc gattagtgtt cgaacatggc agagatcgat
     4741 tcttcgttgc tgatacgacg aatcgcttcg gccagcatac ctgacagggt cagagtacgc
     4801 acgttcggca gtgatttgat ttcatcgctc agcggaatgg tatcgcagac aacgacttca
     4861 tcaattacag agttacgcag gttgttcgcc gcgttgccag agaagatcgg gtgagtcgcg
     4921 tacgcaaata cacgtttagc accacgttct ttcagcgctt cagcagcttt acacagcgta
     4981 ccgccagtgt cgatcatatc atcgaccagt acgcagtcac gacctgcaac gtcaccgatg
     5041 atatgcatca cctgggaaac gttcgcacgc ggacgacgtt tgtcgatgat tgccatatcg
     5101 gtatcgttca gcagcttagc gatagcgcgg gcacgcacaa cgccgccgat gtccggagaa
     5161 accacaattg ggttatccag attcagctgc agcatgtctt ccagcaggat cgggctacca
     5221 aatacgttat caaccggaac gtcgaagaaa ccctgaatct gttcagcgtg cagatccact
     5281 gtcagcacac ggtcaacacc gacgctggag aggaagtctg caaccacttt cgcagtgatt
     5341 ggtacacgag cggaacggac gcgacggtcc tggcgcgcat agccaaagta ggggataaca
     5401 gcggtgatac gacctgcgga agcacgacgc agggcatcaa ccataacgac taattccatc
     5461 aggttgtcgt tagtaggggc acaagtggac tggatgatga aaatatcacc accgcgtaca
     5521 ttttcgttaa tttgtacgct gacttcgccg tcgctaaagc gacctacagc ggcgtcgccg
     5581 agtgaagtgt acaggcggtt ggcaatacgt tgtgctagta ccggggtggc gttaccagca
     5641 aaaagcttca tatcaggcac gagaagaacc tcaggcatgc gtccattggt ggaaagaatc
     5701 tgccgaaaac tgtgcgggcc aggcatgatc ctttccaggc ggtgtattaa agagcgcgat
     5761 gcaacgtctg gaacaaggtg acgttgtcac cgaaactcag cttgcccggc ttaaagcatg
     5821 gctctgtgca atggggaaag attaacgcct ttcgccacaa agccattgag ccattccggg
     5881 gcttgctcta gcacctggcg ggcttcagac tctgtatcaa attcagcaaa gacacaggcc
     5941 cctgtcccag tcaggcgcga cggggcgtat tttaacagcc aggaaagcac cgcatcaacc
     6001 tcgcgaaaac gttttcttgc gataacctcg caatcattgc tgaattcaca ttttagcaac
     6061 gtttctattg acctttttgg cgtattgcgc gggagttcag gatctttaaa aatcaccgga
     6121 gtcggaatac ttacaccagg gtgcgccacc agataccact tctctggcgg atccaccggc
     6181 gttagtattt caccaacgcc ttcggcaaac gcggcatgcc cccgaacaaa gacaggaaca
     6241 tctgcgccca gcgtcagccc catttccgcc agctcatcca tgcttagccc gcattgccag
     6301 agatgattta atgccaccag gaccgtcgcg gcattggatg aaccaccgcc gagaccgccg
     6361 cccatcggca aacgcttgtc aatgctgata ttcgcaccgc ttcccgtcgg aagacgcccg
     6421 ctgtctgccg cagttttcat caacaaccgc gctgcgcgaa cgatcaggtt atcttcatgt
     6481 tccacgcctt caacgggcgt taacagacga atatccccat cgtcacgaag ctcaatgctg
     6541 atggtgtcgc cgtaatcaag aaactgaaac agcgtttgca gcgtgtggta accatccgca
     6601 cgctgaccgg taatgtataa aaacagatta agttttgccg gagagggcca ctgtgtccgc
     6661 attatttcac tatccagtta tccattttta acttgatgcg ttgaccaccg tcggtgagtt
     6721 ccatattggt tggcatcgca ggttgcgttt tggtgtcata accaccataa acaaccttcc
     6781 agtttttgcc attctggctg taggtaattt cgctcaggcg gtactggtcg tccagtttgt
     6841 agtcggttgc atcacccggt aaacctaaaa tccactggcg caagctgttg agcggaattg
     6901 gcattccggt caatttgcca atcatctctt cggcgtcatc ggcggtataa cgctgacctt
     6961 tattgtcgac taactgcacg ttacccggtt gagcattcag ctccagttcc gtgctgccca
     7021 atgggttagt gagcagcaga cggtagcgat cctggccggt ttgctgccag aaaaagcggg
     7081 cgtacacttt ttgttggtca gaaatataag cgaacgcgcc gcgagtctga tactgattaa
     7141 gattgcgcac gtcttgctga tgctgacgcc attgtggcga atccgggctt ttgccaggac
     7201 ctttgggcgt ggtaacggaa caggcagtga gcacaagagc agccagcggt agcaggcgga
     7261 taagacgaaa atcgggcagg ggcatagtga tgacaagtcc ttgagatacg ttgcagttat
     7321 aacccttaat gctagcgtta ccgtccgcta tcgtctatgt tcaagttgtc ttaattgcca
     7381 gaatctaacg gctttcggca attactccaa aagggggcgc tctcttttat tgatcttacg
     7441 catcctgtat gatgcaagca gactaaccct atcaacgttg gtattatttc ccgcagacat
     7501 gaccctttta gcactcggta tcaaccataa aacggcacct gtatcgctgc gagaacgtgt
     7561 atcgttttcg ccggataagc tcgatcaggc gcttgacagc ctgcttgcgc agccgatggt
     7621 gcagggcggc gtggtgctgt cgacgtgcaa ccgcacggaa ctttatctta gcgttgaaga
     7681 gcaggataac ctgcaagagg cgttaatccg ctggctttgc gattatcaca atcttaatga
     7741 agaagatctg cgtaaaaacc tctactggca tcaggataac gacgcggtta gccatttaat
     7801 gcgtgttgcc agcggcctgg attcactggt tcttggggag ccgcagatcc tcggtcaggt
     7861 taaaaaagcg tttgccgatt cgcaaaaagg tcatatgaag gccagcgaac tggaacgcat
     7921 gttccagaaa tctttctctg tagcgaaacg cgttcgcact gaaacagata tcggtgccag
     7981 tgctgtgtct gtcgcttttg cggcttgtac gctggcgcgg cagatctttg aatcgctctc
     8041 tacggtcaca gtgttgctgg taggcgcggg cgaaaccatc gagctggtag cgcgtcatct
     8101 gcgcgaacat aaagtacaga agatgattat cgccaaccgc actcgcgaac gtgcccaaat
     8161 tctggcagat gaagttggcg cggaagtgat tgccctgagt gagatcgacg aacgtctgcg
     8221 cgaagccgat atcatcatca gttccaccgc cagcccgtta ccgattatcg ggaaaggcat
     8281 ggtggagcgc gcattaaaaa gtcgtcgtaa ccaaccaatg ctgttggtgg atattgccgt
     8341 tccgcgcgat gttgagccgg aagttggcaa actggcgaat gcttatcttt atagtgtgga
     8401 cgatctgcaa agcatcattt cgcacaacct ggcgcagcgt aaagccgcag cggttgaggc
     8461 ggaaactatt gtcgctcagg aaaccagcga atttatggcg tggctgcgag cacaaagcgc
     8521 cagcgaaacc attcgcgagt atcgcagcca ggcagagcac gttcgcgatg agttaaccgc
     8581 caaagcgtta gcggcccttg agcagggcgg cgacgcgcaa gccattatgc aggatctggc
     8641 atggaaactg actaaccgct tgatccatgc gccaacgaaa tcacttcaac aggccgcccg
     8701 tgacggggat aacgaacgcc tgaatattct gcgcgacagc ctcgggctgg agtagcagta
     8761 catcattttc tttttttaca gggtgcattt acgcctatga agccttctat cgttgccaaa
     8821 ctggaagccc tgcatgaacg ccatgaagaa gttcaggcgt tgctgggtga cgcgcaaact
     8881 atcgccgacc aggaacgttt tcgcgcatta tcacgcgaat atgcgcagtt aagtgatgtt
     8941 tcgcgctgtt ttaccgactg gcaacaggtt caggaagata tcgaaaccgc acagatgatg
     9001 ctcgacgatc ctgaaatgcg tgagatggcg caggatgaac tgcgcgaagc taaagaaaaa
     9061 agcgagcaac tggaacagca attacaggtt ctattactac caaaagatcc tgatgacgaa
     9121 cgtaacgcct tcctcgaagt ccgtgccgga accggcggcg acgaagcagc gctgtttgca
     9181 ggcgatctgt tccgtatgta cagccgttac gccgaagccc gccgctggcg ggtagaaatc
     9241 atgagcgcca gcgagggtga acatggtggt tataaagaga tcatcgccaa aattagcggt
     9301 gatggtgtgt atggtcgtct gaaatttgaa tccggcggtc atcgcgtgca acgtgttcct
     9361 gctacggaat cgcagggtcg tattcatact tctgcttgta ccgttgcggt aatgccagaa
     9421 ctgcctgacg cagaactgcc ggacatcaac ccagcagatt tacgcattga tactttccgc
     9481 tcgtcagggg cgggtggtca gcacgttaac accaccgatt cggcaattcg tattactcac
     9541 ttgccgaccg ggattgttgt tgaatgtcag gacgaacgtt cacaacataa aaacaaagcg
     9601 aaggcgcttt cagtgctcgg tgctcgcatc cacgctgctg aaatggcaaa acgccaacag
     9661 gccgaagcgt ctacccgtcg taacctgctg gggagtggcg atcgcagcga ccgtaaccgt
     9721 acttacaatt tcccgcaggg gcgcgttacc gatcaccgca tcaacctgac gctctaccgc
     9781 ctggatgaag tgatggaagg taagctggat atgctgattg aaccgattat ccaggaacat
     9841 caggccgacc aactggcggc gttgtccgag caggaataat ggaatatcaa cactggttac
     9901 gtgaagcaat aagccaactt caggcgagcg aaagcccgcg gcgtgatgct gaaatcctgc
     9961 tggagcatgt taccggcaga gggcgtactt ttattctcgc ctttggtgaa acgcagctga
    10021 ctgacgaaca atgtcagcaa cttgatgcgc tactgacacg tcgtcgcgat ggtgaaccca
    10081 ttgctcattt aaccggggtg cgagaattct ggtcgttgcc gttatttgtt tctccagcga
    10141 ccttaattcc gcgcccggat acggagtgtc tggtggagca ggcactggcg cggttgcctg
    10201 aacaaccttg ccgtattctc gatctcggga cggggaccgg ggcgattgcg cttgcgctgg
    10261 ctagcgagcg cccggactgc gaaattatcg ctgtagatcg tatgcctgat gctgtctccc
    10321 tggcacaacg taatgcccag catctggcga tcaaaaatat ccacattctg caaagcgact
    10381 ggtttagcgc gctagccggg cagcagtttg cgatgattgt cagcaatccg ccgtatattg
    10441 acgagcagga cccacatctt caacaaggcg atgtccgctt tgagccgctc actgcgctgg
    10501 ttgcggcaga cagtggaatg gcagacatcg tgcatatcat cgaacagtcg cgtaacgcgc
    10561 tggtatccgg cggctttctg cttctggaac atggctggca gcagggcgaa gcggtgcgac
    10621 aagcatttat cctcgcgggg tatcatgacg tcgaaacctg ccgtgactat ggtgataacg
    10681 agcgcgtaac gctcggccgc tattatcaat gacaagtttt tctacactgc ttagtgttca
    10741 tcttattagt atcgcgcttt ctgttgggct attaacctta cgtttctggc tacgttatca
    10801 gaagcatcca caggcatttg ctcgctggac gcgcattgtg ccgccggttg tcgatacggt
    10861 gttattgtta agcggcattg cgttgatggc taaagcgcac atcctgccat tttccgggca
    10921 ggcacagtgg ctgactgaaa agctgtttgg agttatcatt tatatcgttt tgggttttat
    10981 tgcactcgat tatcgtcgta tgcacagtca gcaggcgcgc attattgcct tcccgctggc
    11041 gttggtggtg ctgtacatca tcattaaact cgccaccaca aaagtaccgt tactggggta
    11101 agttatgaga tcgttagctg atttcgaatt taataaagcg ccattgtgcg aaggcatgat
    11161 cctggcttgc gaagcaatcc gccgcgattt tccctcgcaa gatgtttacg acgaactgga
    11221 gcgtctcgtt agtctggcga aggaagaaat cagccagctt ctgcctttag aagagcagct
    11281 ggaaaaactg atcgcgctgt tttacggcga ctggggattt aaagcctcac gcggcgttta
    11341 tcgtctttcc gatgcattat ggctggacca ggtgttaaag aatcgacagg gcagtgcggt
    11401 atcattaggt gcggttttat tatgggtcgc gaatcgtctc gatttgccgc tgctgccggt
    11461 gattttccct acgcagctga tattgcgcat tgaatgtccg gatggcgaaa tttggctgat
    11521 taatcctttt aacggtgaat cgttaagcga acatatgctg gacgtatggt taaagggaaa
    11581 tatcagcccg tcggcggaac tgttttatga agaccttgat gaagctgata acattgaggt
    11641 aatccgcaaa ttgctggata cactcaaagc ctcgttgatg gaagaaaatc agatggagct
    11701 ggcgttacgc accagcgaag ctttattaca attcaaccct gaagatccct atgaaattcg
    11761 cgatcgcggg ttgatttatg cgcaactgga ttgcgaacac gttgcgttga acgatttaag
    11821 ttatttcgtt gaacagtgtc cggaagaccc gatcagcgaa atgatccgtg cgcaaataaa
    11881 taacatcgcg cataaacata ttgtgctgca ttaattaatc gacattttac tcaagattaa
    11941 ggcgatccta tgaaacaaaa agtggttagc attggcgaca tcaacgtagc aaatgacctg
    12001 ccgttcgtac tgtttggcgg tatgaacgtg ctggaatctc gcgatctggc gatgcgcatt
    12061 tgcgagcact acgtaactgt gacccagaaa ctgggtatcc cttacgtgtt caaagcctct
    12121 tttgacaaag ccaaccgctc ctccatccac tcttatcgtg gaccgggcct ggaagaaggg
    12181 atgaaaatct tccaggagtt gaagcagact tttggcgtga aaattatcac cgacgttcac
    12241 gaaccaagtc aggcacagcc cgttgctgat gtcgtggatg tgattcagtt gccggcgttt
    12301 cttgctcgcc agactgacct ggttgaagcc atggcgaaaa ccggtgcggt aattaacgtc
    12361 aagaaaccac agtttgtcag cccggggcag atgggtaata tcgttgataa attcaaagaa
    12421 ggcggcaacg aaaaagtgat tctttgcgat cgtggtgcta acttcggcta tgacaacctg
    12481 gttgtcgata tgctgggctt cagcattatg aagaaagtgt ctggtaactc gccggtgatt
    12541 ttcgacgtga cccacgcact gcaatgccgc gatccgtttg gcgcagcttc cggtggtcgt
    12601 cgtgctcagg tggctgagct ggcacgagcc ggtatggcgg taggtctggc ggggctgttt
    12661 attgaagcgc atccggatcc ggaacatgcg aaatgtgatg gtccatccgc gctgccgctg
    12721 gctaaactgg aaccgttcct caagcagatg aaagcgattg atgatctggt gaaaggtttc
    12781 gaagaactgg ataccagcaa gtaatctttt ttgcttgaaa aataaagtct tagcgttctg
    12841 cgttaagact tttttcatgg gtgccagata caaaaa
//
LOCUS       DADMGC010000114        12505 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.114, whole genome shotgun sequence.
ACCESSION   DADMGC010000114 DADMGC010000000
VERSION     DADMGC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.114"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(66..2015)
                     /locus_tag="JNZ73_003178"
     CDS             complement(66..2015)
                     /locus_tag="JNZ73_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY8733364.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDATAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2100..2436)
                     /locus_tag="JNZ73_003179"
                     /pseudo
     CDS             complement(2100..2436)
                     /locus_tag="JNZ73_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
     gene            complement(2436..2792)
                     /locus_tag="JNZ73_003180"
     CDS             complement(2436..2792)
                     /locus_tag="JNZ73_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8733365.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2792..4288)
                     /locus_tag="JNZ73_003181"
     CDS             complement(2792..4288)
                     /locus_tag="JNZ73_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8733366.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAH
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4285..4446)
                     /locus_tag="JNZ73_003182"
     CDS             complement(4285..4446)
                     /locus_tag="JNZ73_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005288483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HAY8733367.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(4456..5019)
                     /locus_tag="JNZ73_003183"
     CDS             complement(4456..5019)
                     /locus_tag="JNZ73_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733368.1"
                     /translation="MKLTTIIAALRERCPRFEERVGGAAQFKAIPDAGKLRLPAAYVV
                     PSDDAPGEQKSQTDYWQDLTEGFSVIVVLSNERDEKGQWAAYDAVHDVRRELWKALLG
                     WMPDPQGGEIVYVGGTLLDLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLS
                     IDVDYIDPGTGPDGDIEHHLEMRFPQN"
     gene            complement(5016..5540)
                     /locus_tag="JNZ73_003184"
     CDS             complement(5016..5540)
                     /locus_tag="JNZ73_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733369.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERQRHWTQELLSS
                     ELQRSLRPVKRKHK"
     gene            complement(5506..5922)
                     /locus_tag="JNZ73_003185"
     CDS             complement(5506..5922)
                     /locus_tag="JNZ73_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY8733370.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            complement(5919..6242)
                     /locus_tag="JNZ73_003186"
     CDS             complement(5919..6242)
                     /locus_tag="JNZ73_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY8733371.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            complement(6287..8017)
                     /locus_tag="JNZ73_003187"
     CDS             complement(6287..8017)
                     /locus_tag="JNZ73_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY8733372.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGHGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNGEVETLNGK
                     LTELENLKSDLEAELAEVKRPAGGTQNKVAGEHKEAFIGFMRKGREDGLRELERKALQ
                     VGNDEDGGYAIPEELDRTILTLLKDEVVMRQEATVITLSGSDYKKLVNLGGTTSGWVG
                     ETEARPETATSKLGLIEPFMGEIYGNPQATQKMLDDAFFNVEDWINSELALEFAEQEE
                     IAFTSGDGSKKPKGFLAYESTDEDDKTRAFGKLQHIASGAASGVTADAIIKLIYTLRK
                     AHRSGAKFMMNNSSLFAIRLLKDNDGNYLWRPGIELGQPSSLAGYGIVENEQMPDIAA
                     DAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQAIKLMKI
                     GAATRQKAAA"
     gene            complement(7995..9236)
                     /locus_tag="JNZ73_003188"
     CDS             complement(7995..9236)
                     /locus_tag="JNZ73_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY8733373.1"
                     /translation="MFFQGLFQRKNNTPVTTPGMLAEELGLSYDTYTGKRISSQRAMR
                     LTAVYSCVRVLAESVGMLPCSLYKISGTLKTRAVDERLHKLISAKPNGYMTPQEFWEL
                     VIVCLCLRGNFYAYKVKALGEVVELLPIDPGCVEPKLNSQWQPVYQVTFPDGSVDVLT
                     QDEIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFGNGAVTSGVLRTEQKL
                     TPDAYERMKRDFEERHLGLGNAHRPMILEMGLDWKPVALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRESKQGKFY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(9236..9418)
                     /locus_tag="JNZ73_003189"
     CDS             complement(9236..9418)
                     /locus_tag="JNZ73_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733374.1"
                     /translation="MFLIILTPLVGVLGALLLSYGTWLIYPPAGFVVAGALCLCWSWL
                     VARYLDRGHRVASGGE"
     gene            complement(9430..11187)
                     /locus_tag="JNZ73_003190"
     CDS             complement(9430..11187)
                     /locus_tag="JNZ73_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY8733375.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEERGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSAGIGLYMTGADGEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPRHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVAMFTAMSRMLVNG
                     GEPEPDLSEHLVSVGIRSL"
     gene            complement(11187..11669)
                     /locus_tag="JNZ73_003191"
     CDS             complement(11187..11669)
                     /locus_tag="JNZ73_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY8733376.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDAKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNIAGPDDVDPLAELLKARD
                     "
     gene            complement(11817..12167)
                     /locus_tag="JNZ73_003192"
     CDS             complement(11817..12167)
                     /locus_tag="JNZ73_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY8733377.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(12210..>12505)
                     /locus_tag="JNZ73_003193"
     CDS             complement(12210..>12505)
                     /locus_tag="JNZ73_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY8733378.1"
                     /translation="QRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQS
                     VREATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
BASE COUNT         2818 a         3715 c         2899 g         3073 t
ORIGIN      
        1 aacaaactcc attgttagca tgttacgcga taattttgcc tcatctttca ccacaaaaaa
       61 agaactcacc ttttaaatgg agaataccca acatcatggg tgattttcat aaagggatcg
      121 cctgttttcg gcaaatcgat aacgcgcatt ccaggcggcg cgttgtcaaa cgtcacctta
      181 agttcgctgc gggttgccgg tgaagatgca aggcttaaca gctgattcct ttccagtgat
      241 acattcgggg tgtagcgttt atcgggagat aaatacccat caccacgtgg catctgccat
      301 ccggtcatgt catagacaaa atcatggaac tgtttgcccc actcgtcgaa cttatcattc
      361 agaccaaatc cactgttcag tgcatcagcc acaaaatttt taacaagcca gggatgttct
      421 ttttcaaact cctgcgccat catgcccagt tcaagcaatc caccaatcag actgatcttt
      481 ccgaggccct ttaatttcag ccctgttttt cctgccgccg ttgtccattg attctgggcg
      541 actgttgccg cacgaatact tgccaccgcc ttcacaccaa tatacagggc agaaaccgtt
      601 gctattgttt ttactgcctg ttcccatccc cccattgctc tgacaacctg atcaacctcc
      661 tgccagactt ttttcacaac cggaccgacc gtttcccagt tatcaattat gagatatgca
      721 ccaccgacga gaagcgcaat taatcccttt gccggcgtca tattcatcac gctacccatg
      781 atttttgtta tgcgtgtcaa tgtgccaatc gccacgccca tcgtcagtaa tgccgcacca
      841 gttttcgcga tggtttttac gacctcagga ttttctctga caaacgtgcg cacttcttcc
      901 agaaaaggct tcatctcttt tatgccttca ttgagtgaag gcaggaaggt ttcccccagc
      961 gtggaagaaa tcgcattgat ctggttctgc agcagcaaca actgattttc tgtcgtcgcg
     1021 gcacgggcgg catattcttt ctgcattgag ccgccatact gctgcgcatc cgccacacga
     1081 ttaaagttgg ttcgcaacaa atccagattc gtgagcagcg gcgcaatggc ccccagggat
     1141 tccttcccga acagcgcttt cagcacagca gcctgttttt ctttcggcac ttttgccaga
     1201 gaatccagta cgtgcagcat ggccccgcgg gcgtctttct gcatatccgc cgccagtttt
     1261 ttcgggctaa tgcgcaacga gcggagcact tttttctgcg atttggtggc agaatccctc
     1321 gctgtcagcg acaacataaa attctttatc cccgtcgcag caatttctga ctccaccccc
     1381 attccggcaa tggttgcccc cattgcggca atctctccgg aggccacacc cgcaacactg
     1441 cctaaagggc caatacgggt cacaacatca gaaatctttt tcgcacttgc aggaccggta
     1501 ttgccaagat agttaatctt atccgcaagc cctgccacct ctccctgtgt cagtttaaag
     1561 gcagtgcgcc actgcgccat catctgaccg gattcttctg ccgtggcgtc aaaggccacg
     1621 cccatcttca cggcatcgtc agtaaactgc atcagctcgt cacgggcgat accagcctga
     1681 ccacccgccg ccacaatttc ggcgatacct tccgccgaca tgggcagttc tgttgacagg
     1741 tcgcgcacct gctccgtcat tgccttaaac gcttccggcg tatccagacc atccaccact
     1801 ttccggacat ccgccatttt tgattcaagg gcaatggcag atttgaccgg gagcgccagc
     1861 gcccccagta ctgcggttcc ggcacccgct gcgcccagtg acaggctggc aaactccttt
     1921 ttaaaacctt tcagctggcg ctgcattccc ttcagcggtg ccgatacctt atccacggca
     1981 gtgatgatcg ccttcagctg aaaactgtca gccatgcttc atctcctcgt taatgcggac
     2041 ggcctcggct tccagttctg taaactgcga aacagccacc cgtcgcagct ccagtggatt
     2101 cagtttccag aaccaggcaa cattgtagag tcgtttcccg gaggtgcttc ccgtctccga
     2161 ctgggtaaaa aaacgcagga tctgcatgct ggtcttaaaa atatccagtt ttgccatctg
     2221 cgccgccgat gaacgcggga tccccgccag caacgggata tatttcagtg ccacctggct
     2281 gtccagttta atactgcctt caccggaaat aatgaaagga aaacccagcg cctcgatttc
     2341 gtcatacgtg ggctcgcgca actccagcac atgcagcatt tcgttatgcg ctgtcaccgg
     2401 tttttttaaa actatttctg taacgttcat tgccattact gataccctcc ctcttcgccg
     2461 tggaattcaa gatctgccgt gccttcttcg gcattatgat tagcctctcc gtgcagccat
     2521 gccgccgaaa gcacatacac catgccgttt gccagctcag cggtaatcgt catctggtct
     2581 gaggtggtaa ttttgtcgac cggaaagttt ttgggcactt taaacgtgcc cttaacgtaa
     2641 ggtgaccgcc aggtctcctt gtaatccaca tcccctgcca tgccgacgat gtcatcccgg
     2701 acattggtgt tcatcggcac ttcaatgcca cctgtcagcg ataactgctg accatccact
     2761 ttaaaaaaac aggttccggc gatctttgcc attatgctga ctcctctgaa tactgaagac
     2821 ggaactggtt aaccaccgca aagacacgta actgattaac ataatccggc gggaacagcg
     2881 tattcagtcg gttcgggtta tctgcatcac gctcaactat cagatactgt ttaaacagat
     2941 cgtaattttc cacaataccg gcacgctcca tctgacgata tgtcgccaga agttcccctt
     3001 tgataacggc aggagtgaca atcgcctgcc ccggaccaaa acgggtacca tcatttgcca
     3061 gcttgtgacg tccgtacttg cttgtgatga ccgatttcag tttgcgaaga acatgtgcac
     3121 tggtatgcag agtttcactg tccagatagc tgttgtctgc cacgccatac gcattctttt
     3181 tgtaggtggt tacggaacgc tggatctgca acgtgccgcc ttccacataa gccgtcgcca
     3241 cgccgtgaga taaaagggtc tgctgctctg tcatgatgaa tcgcttacct ttcggtgccg
     3301 gaagcatccc caccagctcc cccgtctgtg tcggacgggc aggatcattc cggataaata
     3361 ccgcttcacg ggcaaggcga ctggcaacca gttcatcgac aggcgactgg gtttcttttt
     3421 cgtaaccggc aagcgtgata tgttgctgat tatgcatatc tccggcacca accagctctg
     3481 acagcgttcc cagttttgcg gtatagacat gcccgtataa ctggcgcgca taactccagc
     3541 gaccgctgct gtcattcatt tcggtcatca tcatattgat ggaggcggca tcgttgaacg
     3601 gcagaccgat aaagtcgaat gcctcatcgc ccatagcagc aacagcggcg gtaagatcag
     3661 gcgctccgct accggcagca ccgacttccg tcaccacccg aagccctgct ggcagaatct
     3721 caccaccacc agaaccataa taattcagac agaccggtaa ctcattacca tacagcccct
     3781 tatgacgggc agtcagcgtc accacgcctg catcagatga cgccgtaaac ggcagggtga
     3841 taaccccgtt taccgcttcc ttaatcgcgg tggcaaccgc agtggcatca tcgccattca
     3901 ccacaggcac ctgtacacgg gagcgcccga catacagact cagggtgccg ctttcctctg
     3961 cttctccggc aaccgtcacc ctgaccgtcg ccgccgcccc tctggcttcc ggtactgcaa
     4021 taacatacag ttcaccgaaa ggatctgtct gacggtagac ttccaccata cgcgccagct
     4081 ggctccccgc cccacaaatc tggcgggcat aatctgccga tggcatcagc accaggctgt
     4141 taacctcaat ggctgcatcg ttgctggcat gcccgatcag taatgcaggc gcgctggtca
     4201 ccgctgtatt tgccgcagaa ttgttcatct cggcgtagaa caacggcacc agcgtattcg
     4261 acggaacagt attaaaactt atcgtcatga ttttttagcc ttatcctgtt taacacgtac
     4321 cacatcaccc gccgctatac ggcggaacca gtagctgctt tcttccacat ttcgcccttc
     4381 ggaaggcaaa aggtctccac gggcagggtc aggaaccgac cgccctttca ggggtttcac
     4441 aaacatgagg gactcttaat tctgagggaa acgcatttcc agatggtgct caatgtcacc
     4501 atccgggcca gtaccaggat cgatgtaatc cacatcaata ctcagcaggg ataaatccgg
     4561 cagggcattc acgtcctctg cctgtcgcgt gtcttcttcc gtgatttcat acttcgccgt
     4621 aaaatcaaac tggtaataca gttcgtaacg gttcagatcc agcagggtgc caccgacata
     4681 aacaatctcg cccccttgcg ggtccggcat ccatccaagc agggctttcc agagttctct
     4741 ccggacatca tgaacggcgt cataggctgc ccactgccct ttttcatcac gctcattgct
     4801 gagcaccaca atcacggaga agccttcagt caaatcctgc cagtaatcgg tctgtgattt
     4861 ttgttccccc ggcgcatcgt cagagggaac cacataggca gcaggcaggc gaagttttcc
     4921 ggcatcaggg atcgccttaa actgcgccgc gccaccaaca cgctcctcaa aacgcgggca
     4981 tcgctcacgc agcgccgcaa ttatcgttgt cagtttcatt tatgcttcct ttttaccgga
     5041 cgtaacgaac gctgcagctc actggacaac agttcctgcg tccagtgacg ctgacgctca
     5101 ataacgtcag ccataaagtt attacgcggg gccagccgga aagtcgaaga atggtgcttc
     5161 ttctgccgct tatccttttt atccattcca tacgctgaat ggcgaacgcc gtaatacaga
     5221 aacgccggat aataaggagc accttcagga aaacggcgat tcccctgccc gtttttctgg
     5281 tcaggggaaa ttttcaccat cagtccgggg cgacgcgtcg tttttttggg aacgtaataa
     5341 ccaatggagc gggccagacg cccggtctga tatcccgggt tctctcccgg accggagcgt
     5401 ccgcgtttaa tcaccagacg tctggcatca cgcatgtaaa cgcgcccgat ttgcacaaac
     5461 gcccgacgca gacgggcgcg attaaactcc agctcctctg gttgtttaaa gtcgacgtgt
     5521 aaaaatgctg tctgattcat ggcattcacc ccgtcgtcgc gctgtacgca gttcttcaca
     5581 ttccagtaat aaaaaacgcc gctgaccgtt caggtcacgt attcgccgga tccggtactc
     5641 ttggccgtaa taaaccactt cgtgatctgc cgtgatgtcg tgccggaaac ggatcgtaaa
     5701 ataatgcgta acgatatttt ctgtctgcac tgagccctga taagcggcag cgcctggctg
     5761 agccaccttt gcccagacat caaacgactc cggatacgtt ggctccgtac caaaatcagc
     5821 ggtgggttca tccacacgaa ggcggatctt tatccggcga ttcagctctc cgggatccgg
     5881 taaaaggtaa gtggcactgg tctgactttg cctgattttc atagcgggac aatcctgtaa
     5941 gggccagcaa gccatctgaa actcattggc gtttccagtt tctccacatc ggtgatcgtt
     6001 gagcgatttt cataaaaatg actgaccagc atcagcattg ccagacgaac gtcatcagtc
     6061 agatgcatcc cgtcaggatc atcttccgga atcgtctctt ccggtgcata caacttcctg
     6121 ttcaggtatg tctctgtttt tttctgtacc gcctgtgcca gcagtttcag aaaatcgccg
     6181 tcactgtaca aaccatcatc gagccggaga tgagatttaa tctcctcttc tttcaggagc
     6241 atattttcct cctgtgcccg cgattacgcg ggcacaaaaa accgcattac gcagcggctt
     6301 tctggcgggt tgcagcccca attttcatca gcttaatcgc ctgagaatcc accagcatac
     6361 cgccggttcg cttggtggta taaaaaccca cgaacggttt gttggtgtac gggtcacgca
     6421 ggatacgggt accgatgcga tcaacgatgg tatagccgcg tttgaagttc ccaaacgcaa
     6481 tggcttttgc atcggcggca atatccggca tctgctcatt ctcaacgatg ccataccctg
     6541 ccagagaaga aggctgaccc agctcaatac ccggacgcca caaataattt ccgtcgttat
     6601 ccttcagcag acgaatggca aacaggctgc tgttgttcat catgaacttc gcgccgctgc
     6661 ggtgcgcctt acgcaaggtg taaatcagtt taatgatcgc atcagcggtc acgccggaag
     6721 ccgcgccgga agcaatgtgc tgaagtttgc caaacgcacg ggtcttgtca tcttcatcgg
     6781 tggactcata agccagaaaa ccttttggtt ttttgctacc gtcgccactg gtaaaggcaa
     6841 tttcttcctg ttcggcaaat tccagcgcca gctcactgtt gatccagtct tccacattaa
     6901 agaaagcatc atcgagcatt ttctgtgtgg cctgcgggtt gccgtagatt tcccccataa
     6961 agggctcaat cagtcccagt tttgaggtgg cggtttccgg acgcgcttcc gtttccccca
     7021 cccatccgga cgtcgtgccg cccagattca ccagtttttt ataatccgag ccactcagag
     7081 tgattacggt ggcctcctgg cgcatcacca cttcatcttt cagcaacgtc agaatggtgc
     7141 gatccagttc ttccggaatg gcataaccac catcttcatc attacccacc tgcagtgcct
     7201 ttcgctcaag ctcacgcagg ccatcttcac gccccttgcg cataaaaccg ataaacgctt
     7261 ctttatgttc cccggcaact ttattttgcg taccacctgc cggacgctta acttcagcca
     7321 gctcagcctc aagatcgctt ttgagatttt ccagttcagt cagttttccg ttgagggttt
     7381 ccacttctcc gttcagtgca gagcccacat cctcagcgtc gcgcagggac agtgcgccat
     7441 agcccccggc catgaatgct ttggcctggg tacgggagag tccgacatca cgcaggactc
     7501 tttcgatttt tttctgttcg gggatttccc cgcgggccag tgcgttcttg acgtcgctga
     7561 tccgcgcctc gtcgttagac gggaacgtca ccagactgac ttcccagagg tcgatttctt
     7621 tcagcagaaa ggcttctttg ctccggtcgt attcccagtc tttcaggacg tacccaatag
     7681 aaaggccggt taacgaaccg gccttcatgt gtgcatgtgc acgttttgcg aggggatcat
     7741 catcaataag caaccgtccc ctgacgtaaa gcccgacatc gtcttccttc atttcggtgt
     7801 aaacaccgat gggttcatcc atgcggtgct gccagagcag cgcaggtaac gcttttctgt
     7861 cactccacgc ccgcagggaa gcagcaaatg ccccggacat caccacatca tcgtggctgt
     7921 cctttacacc aaagacggag ccatgccctt caaactcacc ggagtcactg acagatttca
     7981 gactcagcgg tacatcaaga cgttgtttcg tctgcattgg cgttatcctt ctgcttaccg
     8041 gctttactgc catcggaggg tttcgtggtc atgttcatcg gtgtgagata gacatcccca
     8101 ccgggacgcg gattcatatc ttccaggtcg cggcagtcat tgggagagta aatcccccag
     8161 ttaatcccgg tggcgtaggc ttcaaaacgg gatttcatat ccccgcgcag taacgccccg
     8221 gcgttaaatt tggcgtaaaa cttcccctgt ttgctctccc tgaccagccc tgtattgatc
     8281 cgttgttcaa tacgggtcag atacggcaca agggaatagt tgataaatcc gagccccagc
     8341 tcttcaatat tgttgaaagt ggcgcgatcg gtgttctgca ccatgtgcag cggcacgcgg
     8401 aaaagacgac agatttcttc caactgaaac ttgcgggttt ccaggaactg gctgtcctcg
     8461 gcattcagtg ccaccggctt ccagtccagc cccatttcca gaatcatcgg acggtgcgca
     8521 ttacccagcc caagatgacg ctcctcaaaa tccctcttca tgcgctcata agcatccggc
     8581 gtgagctttt gttccgtgcg caacacaccg gatgtcaccg caccattacc aaacaacctg
     8641 gcaccgtgct cctcggttgc cgctgccagt gaaatggcct cgcgcgcata tgcaatggga
     8701 ttcagcccga ccagtccgtc cagcgtcagg gtgcgcacat gccagatttc atcctgggtc
     8761 aacacatcca cggaaccatc cggaaacgtc acctgataaa ccggctgcca ctggctgttc
     8821 agcttcggtt ccacacagcc cggatctatc ggaagaagct ccacaacttc ccccagcgcc
     8881 ttcaccttgt aagcgtaaaa attcccccgc agacacaggc agacgataac cagctcccag
     8941 aattcctgcg gcgtcatgta gccattgggt tttgctgaaa tcagcttatg cagccgttca
     9001 tccaccgccc gtgttttaag ggtgccgctg attttgtaga gactgcaggg cagcatacca
     9061 acagactcag ccagcaccct gacgcaggaa tagaccgccg tcagccgcat ggcccgctgg
     9121 ctgctgatcc gctttccggt ataggtgtcg tatgacaacc ccagctcttc cgcaagcatc
     9181 ccgggcgttg tgacgggggt gttatttttg cgttgaaaaa gcccctggaa aaacattact
     9241 cacctccgga ggcgacccgg tgaccgcgat cgagataacg cgcaaccagc cacgaccagc
     9301 acagacacag cgccccggca acaacaaaac ctgccggggg ataaatcagc catgtgccat
     9361 acgacagcaa aagcgccccc agcacgccca ccagtggcgt gagaattatc agaaacataa
     9421 tgacctcggt taaagcgagc gaataccaac gctgaccaga tgctcagaca gatccggctc
     9481 cggttcaccg ccattgacca gcatccggct cattgctgta aacatcgcaa cagggccgtc
     9541 gattttggct tccggcgtgg atttgttcgg gaagatattg tcgtttttgt ccggtttcac
     9601 cgtaacgtta gacatcatcc agttcatgac cgggtgatta ctgtgatgga aacgcccggc
     9661 atagaccagt gattccgttt ccttcatggc ctctgacaga ttgcggaccg tctgcggaac
     9721 ttccaccagc ggtatacctt cttcagccag cgccagactg aactgcattg cgctccacgg
     9781 gtcaaatccc agttccctca ggttttcacc gccaatccat tccagtaagt cactttttat
     9841 ctgagcatga tcgataacat caccatccgt cagaatcagc ttatccatct ccgcccactt
     9901 ccggtaaagt tctgcctgct gccgcgagca tcgttccagc cgtccttccg gaagccagaa
     9961 tttaaaatcg gcatgaacat gcccgttatc cgttcgccag agttttgccg ccgcacagat
    10021 atcaatctta tgagcaaggt caacgccgac ccacatggga tacgttttca gctcatgtcg
    10081 tggggcaatg tattcgcact tctcccactt aatcatgtcc atccaggcag actctgctgt
    10141 tacccacaca ttcatgtgtt tggtaaaaaa attcacccgc gcagagacct gttctttcgc
    10201 ttttttcgcc aggcgacgca gatcatccca gcgtttacag atgcccaggc cgggattcgc
    10261 tttctgccag accgtttcat caaacggatc atctccctca tcgagggtgt aaataatcgc
    10321 aaagtaggag tcgtctttta ccgcgccctc cacgtcgctg ttatagccac gcaatacctt
    10381 gatggcgtaa tcacgctgct cgtaacaaat tccttccttg ttaaaccctg ccgtggtgat
    10441 accaaataaa agggactgca gacgggcacc ggttgccgtt tccagaacgt cccacacgtc
    10501 acgggtttta tgtgcatgca gctcatcaat aatggcgcag tggatgttca gaccatccag
    10561 gttgtttgca tctgaagaaa gcggttcaaa ttttgatgcg ctctgctcct gataaatcgc
    10621 cagcttgttg aaatcaaaca accgcccgag tgtcgaccgg gcttttctga ccatattttt
    10681 ggcgtcttca aacacgattc tggcctggtc acgcgtggtt gcggctgaat acacctcagc
    10741 cccgccttca ccatctgccc ccgtcatata cagaccgata cctgctgaca gggttgattt
    10801 tgcgttttta cgggcgactt cgttgtacgc tgtccggaaa cggcgcacca tcaccgggcg
    10861 tccgctgcca tcgctgcgca tgacaacttc cccggtctct tcattcacca gcggaatgac
    10921 aaaaccaaaa atattaatga ggataaacac atgccagtcc atcaactcaa tgggctgacc
    10981 tgccagcgcc ccttttacat ggggcacaaa tttgtagaaa ttcaggatgt gctgcgcacg
    11041 gggttcactg aaataaatcc cccgctcttc gccgtacttc agatcatcaa gaaaacgctg
    11101 gcaggccaga cggacaaatt caccagcgac aatttctcct gcaacaacac gttcggcgta
    11161 gcggatcccg tcagccactt ttgccatcag tctctcgctt ttaaaagttc tgccagcgga
    11221 tcaacatcat ccggtccggc gatatttact ttcgcccggc ttgccggtga cataccaaat
    11281 tctgcaagca tcgcccggat ccgcttccag gcatccgctt tcatcgcagc agccgggtgt
    11341 gccttaatca gcacatcacc gttctgcgtt tccgtgcggt aggtataacc ctcaacatcg
    11401 agggtttcgc agtgatgccg gtattcggtg taggcttcca ccagcaactc gagtgcacgc
    11461 gcatcaagct gagaaatgat cccttccgca ttcagctctt ccgccattcg cctgaaccag
    11521 tactttccct gcgcccctaa atgctgcgga attttaggga gacctttttc gtccttttta
    11581 gcggtttttt tggcgtcttt aacgggacgc tttgagggat tgcctcgtat caaatgcagg
    11641 cgtggcgggg ttttcggggg tcctgacata atcgatttta cctatcaatc gcttgatcgc
    11701 attcccaaaa aaaagttttc gaacctgcgg cgatgcgagg aagggttggc gggcggtccc
    11761 ggacagccag ggctgcaggg atttgacccg cccctcccta caagtgagaa taattatcac
    11821 ctgattcgtt cgcgcgctgt tttcgcttta tggcagggcc agcacagact ctgcaggttg
    11881 ctgtctgcgt ctgttccgcc atgcgctttc gggatgatgt ggtcgacggt tttcgcctcg
    11941 ctcaccacac cgacacgcag acacaactga cacagacctt tatcgcgctt cagaatacgg
    12001 gcacgaatca ccgtccattt tgagccatag ccacgctggt ggcggctcag tccgcgctgg
    12061 tgctgcaccc agccttcacc gcgatgctta tcgcagtaac ccgaactgtc tgtggttgta
    12121 cctgcacatc cacgcttacg gcaggcacgt gggattagtg atggcataaa tacctcatac
    12181 cctgcgaaat gtttaccacg ataaaaaggc tacttaatgc actgagtgcg gatatactcc
    12241 tgtgcccctt ccagttgcat ctgcatcgtc atcagccgct ctctgagggt gaaataatcc
    12301 cgttcagcgg tgtctgccag tcgggggctg gttgcattat ccacgccgga ggcggtggtg
    12361 gcttcacgca ctgactgaca gactgctttg atgtgcaacc gacgacgacc agcggcaaca
    12421 tcagcgcgca gagtttcatt ttcagctttc gcatcagcta actccttcgt gtatttagca
    12481 tcgagtgcat taacatcacg ctgtc
//
LOCUS       DADMGC010000115        12318 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.115, whole genome shotgun sequence.
ACCESSION   DADMGC010000115 DADMGC010000000
VERSION     DADMGC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.115"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..141)
                     /locus_tag="JNZ73_003194"
     CDS             complement(<1..141)
                     /locus_tag="JNZ73_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8733379.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYE"
     gene            517..2157
                     /gene="trg"
                     /locus_tag="JNZ73_003195"
     CDS             517..2157
                     /gene="trg"
                     /locus_tag="JNZ73_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HAY8733380.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTVLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQIQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2195..3118)
                     /locus_tag="JNZ73_003196"
     CDS             complement(2195..3118)
                     /locus_tag="JNZ73_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8733381.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGHAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKESLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINTIRKSMPF"
     gene            3335..4678
                     /locus_tag="JNZ73_003197"
                     /pseudo
     CDS             3335..4678
                     /locus_tag="JNZ73_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            4903..6558
                     /locus_tag="JNZ73_003198"
     CDS             4903..6558
                     /locus_tag="JNZ73_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HAY8733382.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTLQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6698..6922
                     /locus_tag="JNZ73_003199"
     CDS             6698..6922
                     /locus_tag="JNZ73_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HAY8733383.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6985..7521
                     /gene="rimL"
                     /locus_tag="JNZ73_003200"
     CDS             6985..7521
                     /gene="rimL"
                     /locus_tag="JNZ73_003200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HAY8733384.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7516..8496)
                     /locus_tag="JNZ73_003201"
     CDS             complement(7516..8496)
                     /locus_tag="JNZ73_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HAY8733385.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8620..9612
                     /gene="tehA"
                     /locus_tag="JNZ73_003202"
     CDS             8620..9612
                     /gene="tehA"
                     /locus_tag="JNZ73_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HAY8733386.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9609..10202
                     /gene="tehB"
                     /locus_tag="JNZ73_003203"
     CDS             9609..10202
                     /gene="tehB"
                     /locus_tag="JNZ73_003203"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HAY8733387.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10505..11173
                     /locus_tag="JNZ73_003204"
     CDS             10505..11173
                     /locus_tag="JNZ73_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HAY8733388.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(11191..11697)
                     /gene="tnpA"
                     /locus_tag="JNZ73_003205"
                     /pseudo
     CDS             complement(11191..11697)
                     /gene="tnpA"
                     /locus_tag="JNZ73_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11705..>12318
                     /locus_tag="JNZ73_003206"
     CDS             11705..>12318
                     /locus_tag="JNZ73_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8733389.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSF"
BASE COUNT         3186 a         2877 c         3091 g         3164 t
ORIGIN      
        1 ttcatagagg gcaacccatg tcctgacctg ggttcggggg acaccaaaac gtgccgagat
       61 gatcctgtaa ccatcatcag tagtgaagta gtgattcacg acttcaaggc gcttttcaaa
      121 agggtatttt ggctttgaca tattaggggc tattccattt catcgtccaa caaaatgggt
      181 gcagtacagt tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct
      241 gaaaacagaa agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat
      301 aatcacttca cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt
      361 ttagccattc gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt
      421 aaaaatgtga cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt
      481 tctatcagga gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga
      541 ttaggttttt tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc
      601 ttagttctat tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc
      661 gatcagcgtg atgttactgc ggagattgag attcggaccg tgttagcgaa cagttcagat
      721 tttttgcgtt cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa
      781 atggaagcaa tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa
      841 ggttatcgtg cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cctcgacact
      901 gaattaaatc aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc
      961 aaaaatggca tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat
     1021 aatgcttata ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa
     1081 ctggcggaac tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt
     1141 gtgcttgccc tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt
     1201 ccactgcaac atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat
     1261 gatgaaccgg cgggccgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatacag
     1321 cattcactgg ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc
     1381 accagcgaaa tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg
     1441 gctatcgaac aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg
     1501 gataacgcgc atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc
     1561 gggcagacgg tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa
     1621 atttctgaga tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg
     1681 aatgctgccg ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc
     1741 agcgaagtac ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg
     1801 atcagtgaat cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa
     1861 accatgagca ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc
     1921 gccgcctcgg atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg
     1981 gataaggtga cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc
     2041 cttgaagaac aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat
     2101 tctgtgtcgg cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa
     2161 tgttcaagga gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag
     2221 tgttaatcat caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa
     2281 tcggttcgcc tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt
     2341 cgtcttttac agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac
     2401 gagatagcga agcagacagc gtttcgatac aacccgaagg aattttacag ccctggctct
     2461 gcactaatgc atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc
     2521 attccagcac ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa
     2581 ccagcttcaa cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat
     2641 ctgacatccg accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag
     2701 ggttactcat tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta
     2761 taaccgaagg taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat
     2821 tattaagact ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc
     2881 tgactgcatg cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac
     2941 gctcaaacaa gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag
     3001 gttgactcaa attaagggtt tcagctgcgt gccccaaagt tccttgttgt gcgacagcta
     3061 cgaatgtatg aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa
     3121 gcgatgttaa aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg
     3181 attgtaaaat attattaaca attaaagcaa ttatgttaca gcaaaatgga taatattgat
     3241 gttttcgcgg cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg ctgttaccta
     3301 cactccagat tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga
     3361 gattcgggaa cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata
     3421 tggcacgctg ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca
     3481 actgcacgaa aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg
     3541 ggcgattcgc gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat
     3601 ggggatgtac ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac
     3661 agcatttcgg cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc
     3721 cttactccgc cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct
     3781 gcgtcagcgc gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca
     3841 gggcggtttt aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg
     3901 ctggcactag ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg
     3961 gttgattgct gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac
     4021 gctggatatt gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct
     4081 catcgagggg ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc
     4141 actggaagag acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg
     4201 tgaaattgag cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct
     4261 tctgcgtaac gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac
     4321 cttccgcact tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg
     4381 gtaccgtctg acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc
     4441 acagccctta attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt
     4501 gcccgttagc gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag
     4561 tcacggtaat gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga
     4621 gttccagctt taccaagaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat
     4681 tctgaccatc cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca
     4741 tcgacataaa aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa
     4801 taaatattgt ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca
     4861 gcgcgggacc attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt
     4921 attaaaggtt caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt
     4981 tctcaggcag cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac
     5041 ttctccattc tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct
     5101 cgtccgttac ctgacacgct ggcgacaatg acgctgcagg cttataacag tattcaatac
     5161 gacgccgaaa aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc
     5221 catatgggaa tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat
     5281 ctggcgcgtg aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat
     5341 accaaacaat tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc
     5401 cccgaactgg cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt
     5461 gatgatacat atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac
     5521 agtaaagaag agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca
     5581 actaccttta ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc
     5641 actatccatt gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc
     5701 aaagacatta aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat
     5761 gaacgtcgga tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg
     5821 tggcggggca acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc
     5881 aatgcttaca ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc
     5941 tcccattatc aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg
     6001 cgtaacaagt ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg
     6061 ctggataaca ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt
     6121 gcattccagt atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc
     6181 gttatggcga cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac
     6241 tatcccgaaa aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct
     6301 gccgcgccaa aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc
     6361 gaaattctct atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact
     6421 tcggactcca ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc
     6481 agtgaaacat ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac
     6541 gaccgcgtga tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt
     6601 taaacacgat cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt
     6661 tcagatacac cgtgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt
     6721 aatatcccgt ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa
     6781 acttgatcat gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt
     6841 ggtccgcatg aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca
     6901 ggagagcgtc aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa
     6961 cagcatggat tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa
     7021 ttacatgctg ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa
     7081 acctggttac agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga
     7141 aaaacggtgc agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt
     7201 ttcaaagaag atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa
     7261 actgctgaaa tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag
     7321 gcgctgcagg cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc
     7381 aaatgtcggg tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt
     7441 gaaggttgcc tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac
     7501 gcgcgtatta tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc
     7561 gttaatgact tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc
     7621 gtggtcggtt aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc
     7681 ttgtattaca acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc
     7741 aatcaagaca tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg
     7801 cgaactgtaa tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg
     7861 accgtagact ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact
     7921 cgcaaaatca aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc
     7981 ataaatttgt gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg
     8041 gagcaggagt tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc
     8101 aattaaggcg tgaccaaata ttctacattg cccgtaaact aatgagtcat gaatggtgac
     8161 actgtcactt atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt
     8221 tgcgtaaacc tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac
     8281 cgttccgccg aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa
     8341 caccgtttcg cgatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct
     8401 aatggcgata atctgccgta ataacacact ttttttagtg ccatcttcct gataactaaa
     8461 ggctcgctgt tcttcactta ggcgatattt gcgcatagcg ttttcccaca ggtgacttac
     8521 tataaccgta gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt
     8581 tcaaatatac tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc
     8641 aatttgccgg caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg
     8701 cgctatgcca gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg
     8761 gcgatgatca tctggggatt attgactagc gcatttatta cccgactcat acgctttccg
     8821 catagcgtgc tggcggaagt tcgccatcca gtgctgagca gttttgtgag tttgtttccg
     8881 gcaacgacga tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc
     8941 atgttcagtt ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta
     9001 tggcgcggat ctcaccctga ggaagctacc acgcctggac tgtatctgcc gacagttgcc
     9061 aacaacttta tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg
     9121 tttttaggcg caggcgtttt ctcatggcta agccttgaac cggtgatctt gcagcgtctg
     9181 cgcagttcgg gagaattacc cacggcactg cggacatcac tcggcattca gctcgcgcct
     9241 gcgctggtgg cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg
     9301 aaaatgcttt tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg
     9361 tatctctccc agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg
     9421 gcaaccaccg gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg
     9481 gtgccgctgt ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg
     9541 cttctgatgc agggaaaatt gttagtcaga accgagcgcg ccgttttaat gaaagcagag
     9601 gacaaagaat gatcattcgt gacgaaaact attttactga taaatatgaa ttaacccgca
     9661 cacactctga agtactggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg
     9721 gctgtggcaa tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat
     9781 gggataaaaa tgccatgagt atcgccaacg tcgagcgcat taaatccatt gaaaatctgg
     9841 ataatttaca cacccgggtc gttgatctga ataacctcac atttgatgga cagtacgatt
     9901 ttattctttc gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggttgattg
     9961 ccaatatgca acgttgcact aaacctggtg gttacaacct gattgtggcg gcgatggata
    10021 ccgctgatta tccatgtacc gtcggcttca cgtttgcctt caaagaggga gaattacgtc
    10081 gatattacga aggctgggag agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca
    10141 ccgacgccaa cggtaatcgt attaaactgc gtttcgccac gatgctggca cgtaaaaaat
    10201 gacccggtaa gcacaaaacg cgtgaaaatt ccccacgctg agatgattta ctgttcttct
    10261 tttcggtaag catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt
    10321 ttagcagctt aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca
    10381 ttgactatgt cgtctgaaaa ggggctgaaa aatttatttt caccaacact ttttttgcca
    10441 caacacgaag cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact
    10501 attcatgcgt accacatcat ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc
    10561 tggttgtgcc tctaaaatca cccagccaga taaatattct ggttttttaa acaattactc
    10621 tgatttaaaa gaaacaacct cagcaacagg taaacctgtt ttacgttggg tagacccgag
    10681 ttttgatcaa agcaaatatg acagcatcgt ctggaaccca atcacttatt atccggtacc
    10741 aaaaccttct acccaggtag ggcagaaagt tctggataaa attttgaact ataccaacac
    10801 cgaaatgaaa gaagcgatag cgcagcgtaa accagtggtt accaccgctg ggccgcgtag
    10861 tctgattttc cgtggggcca ttaccggtgt agataccagc aaagaagggc tgcaattcta
    10921 tgaagtggtt cctgttgcat tagtggttgc ggggacgcaa atggctacag gccaccgtac
    10981 catggatact cgcctctatt ttgaaggtga gctgattgat gcagcgacta ataaaccggt
    11041 tatcaaagtc gttcgtcagg gcgaaggtaa agacctgaat aacgaaagta cgccaatggc
    11101 tttcgaaaat attaaacaag ttattgatga catggcgacc gatgccacca tgtttgacgt
    11161 taacaaaaag tagttccaga cgcgccatcg ttagatggcg ctttttatcc ggtgcgccgt
    11221 aaaaccccat ccttcagggc ggggatataa ggcgcggttt tccacctgac caggtgtttg
    11281 ctgttgttca atgtattggc ggatgacgga tattggcgca cctccgcagc tactggcaaa
    11341 atagccagga ctccacaaaa cgcctttgta gtaatacctg actgcaatat ctggtcgatc
    11401 tcgtcgcagt aacctacccg atacgccttt gaggctgttt accagactgg atatcgccag
    11461 tttgggaggt tagttaatta acaaatggac gtgatctggt tcgccatcca tttcaaccag
    11521 ttcagcttca aaataagcac atacatttga aaagtaagtg cgtagttttt ctgtcgcgtc
    11581 atggtcaaaa atctggcgtc ggtatctggt gacaaagacc aggtgaacat gcatcaggaa
    11641 aacacaatgc cttccacgcc ggatatcggt ttcttttttc acagaccaaa gtatcatttg
    11701 acctgtgaaa cgattacagg catttaaatt ccagttaaga ccaggtggtc aacaggagtg
    11761 tgaaatgagg cgcttcgcag gcgcatgtcg tttcgttttc aatcgtgctc tggcacgtca
    11821 gaatgagaat catgaggccg gtaataaata catcccttac gggaaaatgg cttcctggct
    11881 ggttgagtgg aaaaatgcca ctgaaacgca atggcttaaa gattctccct cacagccatt
    11941 gcaacagtca ctgaaagacc ttgagcgggc ttacaaaaac ttcttccgga agcgggctgc
    12001 ttttccccga ttcaaaaagc ggggacagaa tgatgcattc cgctacccgc agggcgttaa
    12061 gctcgatcag gaaaacagcc gtatttttct gccgaaactg ggctggatgc gctaccggaa
    12121 tagccgtcag gtcacgggtg ttgtgaaaaa tgtcactgtc agccagtcct gcggtacgtg
    12181 gtacatcagt attcagacag aaagtgaagt atccactccg gctcaccctt cagcatcaat
    12241 ggtcgggctg gatgctggcg tggctaaact cgccacgctg tcagatggca cagtctttga
    12301 gcctgtaaac agttttca
//
LOCUS       DADMGC010000116        12185 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.116, whole genome shotgun sequence.
ACCESSION   DADMGC010000116 DADMGC010000000
VERSION     DADMGC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.116"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(124..291)
                     /locus_tag="JNZ73_003207"
     CDS             complement(124..291)
                     /locus_tag="JNZ73_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733390.1"
                     /translation="MHFRVTGEWNGEPFDRVIEAEDINDCYNHWMIWAQIAHADVTNI
                     RIEELKEHHAA"
     gene            complement(479..1318)
                     /locus_tag="JNZ73_003208"
     CDS             complement(479..1318)
                     /locus_tag="JNZ73_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY8733391.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AEPVAYIFKHPAGKLFWALTDESNKEQADVIPVYAAAPASVVPDNASEPLAYAYKELT
                     PEIMRNHLAVFERYGIAPNDSSTTIQALRIALDGIERSDAMLHGAEPVSQTYKLPPLS
                     SNEVNDAAWKLHNMLTEHGPLNGRQFNNLKGCFYEALKVAMRNYPVTPDGWISCSERM
                     PEDTKMLLAFSQGQIVAAYWNWVMSPIDYKKYRAFTYLSGNILDDVTHWMPLLEPPEQ
                     DGE"
     gene            complement(1320..2006)
                     /locus_tag="JNZ73_003209"
     CDS             complement(1320..2006)
                     /locus_tag="JNZ73_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY8733392.1"
                     /translation="MSEINYQALRKAAQLATQGEWVAFISSGTGTYAVHTPGDKRCED
                     VIKWTGFDGQNNAENNARYIAAFNPEVAQALLDERERNQQYIKRRDQENEDIALTVGK
                     LRVELEGKDSKIANLTAERDALREGEMGDARYSNTRAAADIYFQLVEECEIPAGGSLV
                     EYVDDMREKLESAEKRIAELEEREILLPERSSMLHRTDFHEDYQTVMAYKVSEVIDAI
                     RATGIRIKGE"
     gene            complement(1993..2307)
                     /locus_tag="JNZ73_003210"
     CDS             complement(1993..2307)
                     /locus_tag="JNZ73_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733393.1"
                     /translation="MTITKQRVEKIIYRHEMGLSSDVTAEEVYDLAVLALNLSNIANL
                     KRYELDMGGCDSCGQDCGADMTEDSDGDYVLFDDVVKLFEFDTTTQKLESPAKEATSE
                     RN"
     gene            complement(2304..2789)
                     /locus_tag="JNZ73_003211"
     CDS             complement(2304..2789)
                     /locus_tag="JNZ73_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733394.1"
                     /translation="MKQMTLIEMDGFLKGKCIPSDLKVNETNAEYLVRKFAEAEAKCA
                     ALAAENAGLKAALNHEVIPDTAVEAFNETAIIDHDWNDTSEWSWVENDTDVIRAVLEA
                     IKPETPATDAFLAEIERKAIRKFINSIEHILRDKLSPYDTEEMLETMRIFLEEQGGEQ
                     K"
     gene            complement(2786..2950)
                     /locus_tag="JNZ73_003212"
     CDS             complement(2786..2950)
                     /locus_tag="JNZ73_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HAY8733395.1"
                     /translation="MRGLAYNLGILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMWRNGKK"
     gene            complement(2961..3254)
                     /locus_tag="JNZ73_003213"
     CDS             complement(2961..3254)
                     /locus_tag="JNZ73_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HAY8733396.1"
                     /translation="MPAPQYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(3268..3774)
                     /gene="ssb"
                     /locus_tag="JNZ73_003214"
     CDS             complement(3268..3774)
                     /gene="ssb"
                     /locus_tag="JNZ73_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAY8733397.1"
                     /translation="MASRGVNKVIILGRVGQDPEVRYSPSGTAFANLTIATSEQWRDK
                     NTGEQKELTEWHRVAVSGKLAEVVGQYVKKGDQIYFEGMLRTRKWKDQSGQDRYTTEV
                     HVGINGVMQMLGGIGDSKQQAASRQSQKPQQQSSPAQHNEPPMDFDDDIPFAPVTLPF
                     PRHAIHAI"
     gene            complement(3775..4482)
                     /locus_tag="JNZ73_003215"
     CDS             complement(3775..4482)
                     /locus_tag="JNZ73_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAY8733398.1"
                     /translation="MDLNKFDEPFSPEDIEWRIQQSGKTRDGKVWAMVLAYVTNRAIM
                     KRLDDVCGKAGWRNEYRDIPNNGGVECGISIRIDSEWVTKWDAAENTQVEAVKGGRSG
                     AMKRAAVQWGIGRYLYNLEEGFAQTSLDKKHGWHRAKLKDGTGFYWLPPSLPGWAIPA
                     SDNKPSPENTNQKSPSVDCEQILKDFSDYASTETDKKKLIERYQRDWQLMAGNEEAQA
                     KCVQVMNIRVNELKQAA"
     gene            complement(4737..4889)
                     /locus_tag="JNZ73_003216"
     CDS             complement(4737..4889)
                     /locus_tag="JNZ73_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HAY8733399.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            complement(4874..5005)
                     /locus_tag="JNZ73_003217"
     CDS             complement(4874..5005)
                     /locus_tag="JNZ73_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HAY8733400.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVDVLLCAMK"
     gene            complement(5030..5998)
                     /locus_tag="JNZ73_003218"
     CDS             complement(5030..5998)
                     /locus_tag="JNZ73_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HAY8733401.1"
                     /translation="MSEVTDLVVIEKANAMTVFQSADQIEEILQKVEREVMSFVPDIT
                     TAKGRKEIASLAYKVAQTKTYLDGLGKDLVAELKEIPKLIDANRKTVHDRLDELKAKA
                     RQPLTDYEEEQARIKAEEEAKAAAEALAKQIESDHEIAILMDREFDRQREEARLKAEQ
                     EKREHEERLKREAEEKARAEAEAKAKAEIEAAARREAEAKAAAERAKRERIEAEQRAQ
                     REAKEAAERAEREKQAAIEAERRKAQEEAERIRREAEAKEQARIAEEKRIKDEEERRA
                     KDKAHRKEVNNKILADLIKVGASEDVAKNIITAIVKGEVFATKITY"
     gene            complement(6166..6408)
                     /locus_tag="JNZ73_003219"
     CDS             complement(6166..6408)
                     /locus_tag="JNZ73_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733402.1"
                     /translation="MRVKTMGASLLSGRIFQGTLNTEKGMWVGKREDVTDQAVRAVAE
                     HMMIKDQKYAYETKDGKWLIISHQLVDKLPEEFVDG"
     gene            complement(6611..6943)
                     /locus_tag="JNZ73_003220"
     CDS             complement(6611..6943)
                     /locus_tag="JNZ73_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY8733403.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRTERKNKRTWYSKPRSEIGVTCSGRQKQRG
                     KSIPAYYD"
     gene            complement(7404..7808)
                     /locus_tag="JNZ73_003221"
     CDS             complement(7404..7808)
                     /locus_tag="JNZ73_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733404.1"
                     /translation="MIRIAALLSILLTTSANSECWIVTNLHGYGSMNGDRYEFTKDST
                     EDSVFHVTINGDKSSVYESVSGVYPEMKYTALSSNTMVGEYQSGGGITVETWSITTDK
                     KALYSKVMNIPGMQQLTSTKSFVGDVVGTCNQ"
     gene            complement(7805..8437)
                     /locus_tag="JNZ73_003222"
     CDS             complement(7805..8437)
                     /locus_tag="JNZ73_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAY8733405.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            8541..8756
                     /locus_tag="JNZ73_003223"
     CDS             8541..8756
                     /locus_tag="JNZ73_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8733406.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            8876..9169
                     /locus_tag="JNZ73_003224"
     CDS             8876..9169
                     /locus_tag="JNZ73_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733407.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            9202..9348
                     /locus_tag="JNZ73_003225"
     CDS             9202..9348
                     /locus_tag="JNZ73_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 domain-containing protein"
                     /protein_id="HAY8733408.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            9341..10174
                     /locus_tag="JNZ73_003226"
     CDS             9341..10174
                     /locus_tag="JNZ73_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAY8733409.1"
                     /translation="MSNISNLAEAREARRLQQPHQSSGKGYALLHRKIMDVPFYKDAE
                     AAHLWVHLILKAKHTPEYVMTDAGEILVGRGKLLGGRNSLAFETGLKPDRVQYLLRKF
                     KKLGMIDWVSHGKFSVFSVEKYDDYQSNFVPADYQQITTDKEYNNIISNTDVLESTAA
                     DKKSDKKKPSVSCQDVVDAYHEILPEAPKIRALNDKRKNQIRTFWRKAGVITRQLDGH
                     GFTMQDWRNYLSYVGENCRWMFEERPNHQRGTVWHKKGFDFLLNDNTYLKVREGEHDD
                     R"
     gene            10164..11600
                     /locus_tag="JNZ73_003227"
     CDS             10164..11600
                     /locus_tag="JNZ73_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY8733410.1"
                     /translation="MTDNFYAPPHSIEAEQAVIGGLLLDDDSSERVQKVLAMLKPDSF
                     YSRPHKIIFEEITRMHREQKPVDGLTLFDELERKSLTASVGGFAYIAEIAKNTPSAAN
                     IVAYAMQVRETAMERYAINRMTEATELLYSRNGMTATQKYEAIQAIFTQLTDHAKTGS
                     RRGLRSFGEVMEDWVSDLEKRFDPSGEQRGMSTGIPSLDRMLSPKGLVKGSLFVIGAR
                     PKMGKTTLYSQMAINCAVHEKKPALMFSLEMPGDQILEKLVGQKSGVNPNIFYLPATN
                     DADDGYQGDYDGDFNRAIETANRLSEIDLLYIDDTPGLSLAQIVSESRRIKREKGCVG
                     MILVDYLTLMTAEKADRNDLAYGMITKGLKNLAKELDCVVVLLTQLNRALESRTNKRP
                     LPSDSRDTGQIEQDCDYWVGIHREGAFDDSVPPSETELILRLNRHGNTGTVYCIQANG
                     AIYDTDQQSAEMRRREREEPQSKKKGGF"
     gene            11677..12117
                     /locus_tag="JNZ73_003228"
     CDS             11677..12117
                     /locus_tag="JNZ73_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HAY8733411.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVSEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            12114..>12185
                     /locus_tag="JNZ73_003229"
     CDS             12114..>12185
                     /locus_tag="JNZ73_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HAY8733412.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRS"
BASE COUNT         2949 a         2996 c         2715 g         3525 t
ORIGIN      
        1 ttttcagtcc acatgcgatt gaggcaagag ttgcgctact gatgccaaca tcgccagtcg
       61 attgccattt caaaaccttc atagccaaat ctgacatttc ttgtctccaa taaaaaaccg
      121 ccatcaggcg gcatggtgtt ctttcagttc ctcaattcga atattggtta cgtctgcatg
      181 tgctatctgc gcccatatca tccagtggtt atagcagtcg ttgatgtcct ctgcttcgat
      241 aaccctgtcg aatggctctc cattccattc acctgtgact cggaagtgca tttatcatct
      301 ccataaaaca aaacccgccg tagcgagttc agataaaaga aatccccgcg agtgcgagga
      361 ttgttagttg ctatctgctg cttccttggt cattaccata tccacccaat ttcaccagac
      421 atgattctcg caatcactat tatcaccaaa gtgataatca caactttaac tggcggcatc
      481 attcaccatc ctgctccggc ggttctagca gcggcatcca gtgggttacg tcatccaaga
      541 tatttcctga taaatatgtg aaagctctat attttttgta atcaattgga ctcattaccc
      601 agttccaata tgctgccact atttgacctt gactaaatgc cagtaacatt ttggtgtctt
      661 ccggcattcg ctcactacag cttatccaac catccggagt taccggatag ttgcgcattg
      721 cgacctttaa tgcctcatag aagcaacctt tcagattgtt gaactgacgc ccatttagcg
      781 ggccatgttc agtcagcatg ttgtgtaatt tccatgccgc gtcgtttact tcgttggatg
      841 acaggggagg caacttgtaa gtttggctta caggttcggc accatgaagc atggcgtcgc
      901 tccgctctat gccatccagc gcgattcgta gtgcctgaat tgtggtagag ctatcgtttg
      961 gggctattcc atatcgctcg aatacagcta aatggttgcg cataatctca ggcgtaagct
     1021 ctttgtaagc ataagcgaga ggctctgatg cattatccgg cacaaccgac gcaggcgcgg
     1081 cagcataaac aggaataacg tccgcttgct ctttattgct ttcatccgtt aaagcccaga
     1141 ataatttccc ggccggatgt ttgaaaatat aagcaactgg ctctgcttcc agcgatgcca
     1201 gtgcaattcg tgccagttct tccgcttctt ctgctggcag tacaacgttg ctaccaggtc
     1261 cgtatgtttc gcgccactgc ttgattgtca gtagtcgctc tttggtaata gtggtcatct
     1321 cactctcctt tgatgcgaat accagtagcg cggattgcat cgatgacttc agaaactttg
     1381 tatgccatta ccgtttggta atcctcgtga aaatctgttc gatgaagcat gctgctacgt
     1441 tccgggagca gtatttcccg ctcctccagt tctgctatgc gcttctctgc ggattccagc
     1501 ttctcgcgca tatcgtcaac gtactcgacc agagaaccgc cagcaggaat ttcgcactcc
     1561 tcgaccagtt ggaagtagat atcagctgcg gcccgtgtgt tgctatacct agcgtcgccc
     1621 atctcacctt cacgaagagc atcgcgttcg gcggtaagat tggctatttt gctgtctttg
     1681 ccttccagct caacgcgcag cttccctacc gtaagcgcaa tatcctcgtt ctcctggtcg
     1741 cggcgtttga tgtattgctg gttcctttcc cgttcatcca gtagtgcctg cgctacttca
     1801 gggttgaaag ctgcgatata acgagcgttg ttctctgcgt tgttctgtcc atcaaagccg
     1861 gtccatttga taacgtcttc acatcgttta tcaccaggtg tatgcaccgc atatgtacca
     1921 gtaccagacg aaataaatgc gacccattca ccctgtgttg ccagttgcgc cgctttacgc
     1981 agtgcctggt aattaatttc gctcactggt tgcctccttt gctgggcttt ctaacttttg
     2041 agtggttgta tcaaattcaa acaacttaac cacgtcatca aacaggacat aatcaccatc
     2101 agaatcttca gtcatgtcag cgccacaatc ctgaccgcat gagtcacaac cacccatatc
     2161 aagctcgtat cgcttcaggt ttgcgatatt tgataaattc agcgccagta cagccaggtc
     2221 ataaacctct tcggcagtga catcgctgct cagtcccatt tcatggcgat atatgatttt
     2281 ttctactcgt tgttttgtga ttgtcatttt tgctcgcctc cctgttcttc cagaaaaata
     2341 cgcatagtct caagcatctc ttcggtgtca tacggtgaca acttgtcacg caggatgtgt
     2401 tcaatgctgt taatgaactt gcggattgct ttgcgttcaa tttcagccag aaaagcatcg
     2461 gttgccgggg tttcaggttt aatggcttca agcacggcgc gaatgacatc ggtatcgttc
     2521 tccacccatg accattcgct tgtatcattc cagtcgtggt ctatgatagc tgtttcattg
     2581 aatgcctcga ctgccgtatc cgggattact tcatgattta aagctgcctt cagccccgca
     2641 ttctccgccg ccagcgccgc gcacttggcc tccgcttcag caaatttacg caccagatat
     2701 tcagcgtttg tttcgttaac ctttaaatca cttgggatgc atttaccttt cagaaatcca
     2761 tccatctcaa ttagtgtcat ttgtttcatt tcttcccatt ccgccacatt gcattcagat
     2821 atttgttttg atttactgac ggaaaagaat ttcttttaag caattcctct ctcgatggca
     2881 ttggctttac gcgttggcga ataatcattt ctgccggaag aatgccgaga ttgtatgcaa
     2941 gtcctctcat ggtaaattcc tcagtcatta ctgatagcgc catagcgtga gcggtaatta
     3001 cgcaggcgcg ggtcaatttc agggaagtgg gtatatgtgg ctttgcggaa tggtcggatt
     3061 gatgtctggt aaattcgctc gcgctcttct ttctctgcaa gccatatgca gtggcgaaat
     3121 tccttttcct ctttcgtttc ctgcggtagc gacattatcc ggtcgtagtt tttcctgaat
     3181 ttatccagca cctccgatac ggaattgccg gaacagcggc gcgggtcatc cgcaccatac
     3241 tgaggcgctg gcatgatttt ctcctgatta aattgcgtga atagcgtgac gagggaaggg
     3301 gagagttact ggtgcaaagg gtatatcgtc gtcaaaatcc atcggaggtt cgttgtgttg
     3361 tgctggtgat gattgctgct gtggcttctg tgattgcctg ctggctgctt gttgtttgct
     3421 gtcgccaatg ccgccaagca tttgcatcac gccattaatt ccgacatgaa cctcggttgt
     3481 gtaacggtct tgccctgact ggtctttcca ctttctggtt ctcagcattc cctcgaaata
     3541 aatctgatca ccttttttca catactgccc cacgacctca gccagtttcc cggatacagc
     3601 aacacgatgc cattcagtca attccttttg ctcgccagta tttttatctc gccattgttc
     3661 tgacgtggct attgtcaggt tagcgaacgc tgttcctgat ggtgagtatc gaacttccgg
     3721 gtcttgtcct acccgaccaa ggataatcac cttatttacg cctctgcttg ccatttatgc
     3781 cgcctgtttt agttcgttaa ctctgatgtt cattacctga acgcatttag cctgcgcttc
     3841 ctcgttgcca gccattaatt gccagtcacg ctgataacgc tcgatgagtt ttttcttgtc
     3901 agtttctgtt gacgcataat cgctgaagtc tttcaggatt tgttcgcagt caaccgatgg
     3961 agatttctgg ttggtatttt ctggtgatgg tttgttatct gatgctggga ttgcccatcc
     4021 cggcagcgat ggagggagcc agtaaaatcc tgttccatcc ttcagttttg ccctgtgcca
     4081 cccatgcttt ttatcgagag atgtttgtgc gaaaccttcc tcaaggttat acagataccg
     4141 accgattccc cactgaacgg cagcacgctt cattgcacct gaacgaccac ctttgacggc
     4201 ttctacctgc gtgttttcag cagcatccca tttggttacc cattcggaat caatcctgat
     4261 tgatatgccg cattcaacgc ctccgttgtt gggaatatcg cggtattcat tgcgccatcc
     4321 tgctttgccg caaacatcgt ccaggcgttt catgattgcc cggttcgtga cataagccag
     4381 caccatagcc cacactttgc catcgcgtgt tttaccgctt tgctgtattc gccattcgat
     4441 atcttcaggg ctgaatggct catcgaattt attcaaatcc ataaatcacc tcagaatgga
     4501 catggcccaa gaaaataacg ctgatttaat acttcgactc gggacaaatt aaggcatacc
     4561 cgcattcctt cgcggtcgcc attatggcga taccagagag ctttctgggt gtacatgcgt
     4621 ctctgtaact tgctctcctt cactgtggtt gcaagtgaca tgaatatctc cttcgtttcc
     4681 gattaattct ttcatctgac gaatgaattc ttcgtctgac cagttatctg taaaactcat
     4741 ttcctgcgat accacggaag gttgatagct gacttcatcg ctttatttgc ttcaagccac
     4801 attttggaat caccaataaa tctggctatt actgctttgt tctgtgcagc acgaagcatc
     4861 tggtgattga tggctatttc attgcgcata ataagacatc aactcttttc catccgtcac
     4921 gtaatttacg ggtgattcgt tcaagtaaag attcatttaa ttggaaggca cccatgcgag
     4981 cgcctcccgc gattgcgtaa atcatgggtg gttccttatg ttggttttat tagtaggtta
     5041 tttttgttgc gaatacttcg ccttttacga tggctgttat gatattttta gcaacatctt
     5101 ctgatgcgcc aaccttgata aggtcagcaa gtattttgtt atttacttct tttcggtgag
     5161 ctttatcctt tgctctacgc tcttcttcgt ccttgattct tttttcttct gctattctgg
     5221 cttgctcttt tgcttcagct tcgcgccgga ttcgttcagc ctcctcctgt gcttttcggc
     5281 gttctgcttc aattgccgcc tgcttttctc tttcagctcg ttctgctgcc tcttttgctt
     5341 cgcgctgtgc tcgttgctcg gcttcaatgc gttcacgctt tgcacgttcc gctgcggcct
     5401 tagcttctgc ttctcgcctt gctgctgctt caatttcggc ttttgccttt gcttcggctt
     5461 ctgctctggc tttctcttca gcttctcttt ttaagcgttc ttcatgctct cgcttttcct
     5521 gctccgcttt gagtcttgcc tcttctcttt ggcggtcaaa ttcgcgatcc atcaaaatcg
     5581 ctatttcatg gtcagactca atttgctttg cgagagcttc agctgctgcc ttagcttctt
     5641 cttcggcttt aatccgcgcc tgttcctcct cataatcagt aagaggctgg cgcgccttgg
     5701 ctttcagctc atcaagtcga tcatgcactg ttttgcggtt ggcatcaatt agctttggaa
     5761 tttccttcag ttcagcaaca aggtctttgc caagaccatc gagatatgtt ttcgtctgcg
     5821 caactttata cgccagagaa gcgatctcct ttctgccctt tgccgttgtg atatcaggca
     5881 caaaggacat aacttcacgt tcaacctttt gaaggatttc ttcaatctgg tcggcagact
     5941 gaaatacagt cattgcattt gctttttcaa taacaactaa atctgttact tcactcatat
     6001 ttcccccgtc aaaaaaattg ccctcacact ggagggcaaa gaagatttcc aataatcaga
     6061 acaagtcggc tcctgtttag ttacgagcga cattgctccg tgtattcact cgttggaatg
     6121 aatacacagt gcagtgttta ttctgttgct tattccaaag caaatttaac catcaacaaa
     6181 ctcttctggt aatttatcaa ccagttgatg acttattatc agccatttgc catccttcgt
     6241 ttcgtatgca tatttctggt cttttatcat catgtgttcg gctactgccc tcactgcctg
     6301 atcggtgaca tcttctctct ttcctaccca cattcctttt tcagtgttta atgttccttg
     6361 aaaaatacga ccacttaata ggcttgcgcc catagttttt actctcatgt atcagtcctc
     6421 aaataagtgg tttgctgcga aagtaaatac gcttaagtta cctgttattt atcccaccaa
     6481 gttccgtatc tatctatcca gttacaccaa tcatcgacac tctattttgt tgtgtcgcat
     6541 tttggcaact ggcaagaata tctaccttct ttgtaaagtc ggcgtttgac tttcttgagc
     6601 atggctcacc tcaatcgtaa taagctggaa ttgattttcc gcgttgcttc tggcggcctg
     6661 agcaagtcac acctatttca ctgcgtggct tgctgtacca tgtgcgctta ttcttgcgct
     6721 cagtacgctg caggttgctt tcaatctgtt cgtggtattc agccagcacc gtaaggtcta
     6781 tcggattcag tgcgctttct actcgtgatt tcggtttgcg attcagcgag agaatagggc
     6841 ggttaactgg ttttgcgctt accccaacta acaggggatt tgctgctttc cattgagcct
     6901 gtttctctgc gcgacgttcg cggcggcgtg tttgtgcatc catctggatt ctcctgtcag
     6961 ttagctttgg tggtgtggtg gctggtagtc tagctccagc ttgttgagtc tcattcggag
     7021 gggtataacc ggcaccccag cgatttttcc atgcgacaac gtgcgcgtta tggcggcctt
     7081 atcgcccgcg gctccccatc tcgtccacgc tattgctagc gttgggagcg cttcaccgct
     7141 caacagtagg taagcacttg ccagtgacta gctggcttca ccacacccca aagccttctg
     7201 ctttgaatgc tgcccttctt cagggcttat tttttaagag cctcaccttc atggtggtca
     7261 gtgcgtcctg ctgatggctt aaaattacaa gaaagattgt atgttgtaaa caagaaatat
     7321 tgtgaaaagg ggcgtgaaaa acaaactcca ttgtttttaa atgaaaaata gtttgttttt
     7381 ggttatcgag attgaggtgg ggattactgg ttgcaggttc cgactacatc accaacaaat
     7441 gatttggttg atgtaagttg ttgcatacct gggatattca ttactttgga gtaaagagct
     7501 tttttgtctg tagtgattga ccaagtttca acggttattc ctcctccaga ctggtattct
     7561 cctaccatag tgttcgatga caaagcagtg tatttcatct ctggatagac gccagaaact
     7621 gattcataaa ctgatgattt atcgccattt attgttacgt ggaaaacgga atcttccgtg
     7681 ctgtcttttg taaactcgta acgatcgcca ttcattgacc cgtacccgtg caggtttgtg
     7741 acaatccagc attcagaatt ggcgctggta gttaagagta ttgagagtag cgccgcaatc
     7801 ctgatcatac gaattttacc ctcgcttcca cgacaacacc gataatcttg cagttcccgt
     7861 tgataggagt cataggccat gaagggttca ggcctttcag gtacttctgc ccgccatcta
     7921 taaccagttt cttgaatgtt gcttcgttcg cgtcagtcag tttggctaca acaaggcttc
     7981 cattcactgg ctcgcgtcca gtatctacta acaccatatg accttcaggg atgctttgac
     8041 ctacaggtga ggtcatggaa tcaccttcaa ccttcagcca gaatccatcg cctaataagt
     8101 taacgtcact gtcataccat tcatcaatgt ccttgatatc gtagggttca caagcttcac
     8161 accacgaacc agctctaacc atgctaatca atggatattt ccctttgggt tcaacatgcc
     8221 caacaaatct aacattcgaa tcagaggtgc cattgagcag ccagtcaaca cttacgccaa
     8281 gagctgacgc aagttctggt aaaaagcgtg gtcgcttagt tttaccgttt tcgagctgct
     8341 ctatagactg ctgggtagtc cccacctttt gagcaagttc agcctggtta agtccaagct
     8401 gaattctttt gctttttacc ctggaagaaa tactcataag ccacctctgt tatttacctc
     8461 caatcttcac aagaaaaact gtattttaca aacaagatac attgtatgaa aatacaagaa
     8521 agtttgttga tggaggcgat atgcaaactc tttctgaacg cctcaagaag aggcgaattg
     8581 cgttaaaaat gacgcaaacc gaactggcaa ccaaagccgg tgttaaacag caatcaattc
     8641 aactgattga agctggagta accaagcgac cgcgcttctt gtttgagatt gctatggcgc
     8701 ttaactgtga tccggtttgg ttacagtacg gaactaaacg cggaaaagcc gcttaagaca
     8761 ttcccgctct tacacatccc agtcctgaaa aagggcatca aattaaacca cacctatggt
     8821 gtatgcattt atttgcatac cttcaatcaa ttgttatcta aggaaatact tacatatggt
     8881 tcgtgcaaac aaacgcaacg aggctctacg aatcgagagt gcgttgctta acaaaatcgc
     8941 aatgcttgga actgagaaga cagcggaagc tgtgggagtt gataagtcgc agatcagcag
     9001 gtggaagagg gactggattc caaagttctc aatgctgctt gctgttcttg aatggggtgt
     9061 cgttgacgac gacatggctc gattggcacg acaagttgct tcgattctca ccaataaaaa
     9121 acgcccggct gcaaccgagc gttctgaaca aatccagatg gaattctgag gtcattactg
     9181 gatcaatcca caggagtaat tatgacaaaa caactcagtc cttaccagga caaaattcac
     9241 aaacacatac tacgtgatcg cttcctgtcc agcttcaagc agcctggtcg attccgggct
     9301 gagttggaaa aagtgaagct gatgcagaag gagaaaggtc atgagtaaca tatctaatct
     9361 agccgaagcc agagaggcca gaaggctaca acaaccgcat caaagcagcg gtaaggggta
     9421 tgccttgctg caccgtaaaa ttatggatgt gccgttttac aaggacgcag aagcagcgca
     9481 tctgtgggtt cacttaatcc tcaaagcaaa gcatacgcct gagtatgtaa tgactgacgc
     9541 aggagaaatt ctggtaggca gagggaagct acttggcggt agaaactctc tggcgtttga
     9601 aacaggactc aaaccagatc gcgttcagta cctgcttaga aagttcaaaa aactcggcat
     9661 gattgactgg gtttcacacg gtaaattctc agttttctcg gtagagaaat atgacgatta
     9721 tcagtcaaat tttgtaccag cagattacca gcaaattacc acagataaag aatataataa
     9781 tattatctct aatactgacg tattagagag taccgcagca gacaaaaagt ctgacaagaa
     9841 aaaaccttcc gttagctgtc aggatgttgt cgatgcttac cacgaaatcc ttcctgaagc
     9901 gccaaaaatc cgcgcactga atgacaagcg taaaaaccag atccgaacgt tctggcgcaa
     9961 agccggagtg ataacccgcc agcttgacgg gcatgggttc acgatgcagg actggagaaa
    10021 ttatttgagc tacgtaggcg aaaattgccg atggatgttc gaagagcgcc caaaccatca
    10081 gcgcggaacc gtctggcaca aaaagggatt tgatttcctg cttaacgata atacctacct
    10141 gaaagttcgt gagggtgaac acgatgaccg ataattttta tgcgccgccc catagcatcg
    10201 aggcagagca ggcggtgatt ggtggattgc ttctggatga tgacagcagt gagcgcgtcc
    10261 agaaggttct ggcgatgctg aagcctgatt cattttacag ccgaccacac aaaatcattt
    10321 tcgaagaaat aaccagaatg caccgggagc aaaagccagt agatggcctg acgcttttcg
    10381 atgaactgga gcgtaaatcg ttaacggcgt ctgttggcgg ttttgcttat atcgctgaga
    10441 tcgcaaagaa cacgccaagc gccgcaaaca tcgttgccta tgcaatgcag gttcgcgaaa
    10501 ccgcaatgga acgctacgcc atcaaccgca tgactgaagc gacggaattg ctctattccc
    10561 gcaacggaat gactgcgacg cagaagtacg aagctattca ggcgattttc acgcaactga
    10621 cagaccatgc aaaaacagga tcgcgtcgcg gccttcgctc atttggtgag gtcatggaag
    10681 actgggttag cgaccttgag aagcgatttg acccatcagg cgaacaacgg ggaatgagca
    10741 cagggatccc atcgctggac aggatgctgt caccgaaagg tctggtgaaa ggctctctgt
    10801 ttgtcattgg cgctcgacct aagatgggga aaacgacgct atacagccag atggcaatca
    10861 actgcgcagt gcatgagaaa aagcccgctc ttatgttcag ccttgaaatg ccaggtgacc
    10921 agatactgga aaaactggta gggcagaagt ctggtgttaa cccgaatatt ttttaccttc
    10981 cggcgacaaa tgacgctgat gacggctatc agggtgatta cgatggtgac ttcaacaggg
    11041 cgatcgaaac agccaatcgc ttgagtgaaa tcgacctgct ttacatcgac gacacgccgg
    11101 gattatctct ggctcaaatc gtcagcgaaa gccgtcgaat caagcgagaa aaaggatgtg
    11161 ttggcatgat tctggtcgat tacctgacac taatgaccgc tgaaaaggcc gatcgcaacg
    11221 accttgctta cggcatgatc accaaaggac tgaagaacct tgccaaagag cttgattgcg
    11281 ttgttgtgct tctgacacag cttaaccgcg cactggaaag ccgaaccaat aaacgcccat
    11341 taccaagtga ctcacgagat acagggcaga ttgaacagga ttgcgattat tgggtgggga
    11401 tccatcgtga aggtgctttt gatgacagtg ttccacctag tgaaaccgaa ctaatccttc
    11461 gtctcaatcg tcatggcaat accggcacgg tgtattgcat tcaggcaaat ggcgctattt
    11521 atgacacaga ccaacagtct gctgaaatgc gccgacgtga acgcgaggaa ccgcagtcca
    11581 agaagaaagg aggattctga tgaccatcta catcactgag ctaataacag gtctgctggt
    11641 aatcgcaggc ctttttattt gggggagagg gaagtcatga aaaagctaac ctttgaaatt
    11701 cgatctccag cacatcagca aaacgctatt cacgcagtac agcaaatcct tccagaccca
    11761 accaaaccaa tcgtagtaac cattcaggaa cgcaaccgca gcttagacca gaatcgaaag
    11821 ctttgggctt gccttggtga cgtctctcgt caggttgaat ggcatggtcg ctggctggat
    11881 gcagaaagct ggaagtgtgt gtttaccgca gccttaaagc agcaggacgt tgttcctaac
    11941 cttgccggga atggctttgt ggtaataggc cagtcaacca gcaggatgcg agtaagcgaa
    12001 tttgcggagc tattagagct tatacaggca ttcggtacag aacgtggcgt taagtggtca
    12061 gacgaagcgc gactggctct ggagtggaaa gcaagatggg gagacagggc ggcatgaggc
    12121 gacagcgacg aagtttcacc gacatcatct gcgaaaactg caaatacctt ccaacgaaac
    12181 gctcc
//
LOCUS       DADMGC010000117        12153 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.117, whole genome shotgun sequence.
ACCESSION   DADMGC010000117 DADMGC010000000
VERSION     DADMGC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.117"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            127..996
                     /locus_tag="JNZ73_003230"
     CDS             127..996
                     /locus_tag="JNZ73_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733413.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALPLKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYARTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1199..1552)
                     /locus_tag="JNZ73_003231"
     CDS             complement(1199..1552)
                     /locus_tag="JNZ73_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAY8733414.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1690..3336)
                     /gene="groL"
                     /locus_tag="JNZ73_003232"
     CDS             complement(1690..3336)
                     /gene="groL"
                     /locus_tag="JNZ73_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAY8733415.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3380..3673)
                     /locus_tag="JNZ73_003233"
     CDS             complement(3380..3673)
                     /locus_tag="JNZ73_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAY8733416.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3949..5205
                     /gene="yjeH"
                     /locus_tag="JNZ73_003234"
     CDS             3949..5205
                     /gene="yjeH"
                     /locus_tag="JNZ73_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAY8733417.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLEALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5221..5697)
                     /gene="fxsA"
                     /locus_tag="JNZ73_003235"
     CDS             complement(5221..5697)
                     /gene="fxsA"
                     /locus_tag="JNZ73_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HAY8733418.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6034..7470
                     /gene="aspA"
                     /locus_tag="JNZ73_003236"
     CDS             6034..7470
                     /gene="aspA"
                     /locus_tag="JNZ73_003236"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAY8733419.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7588..8889
                     /locus_tag="JNZ73_003237"
     CDS             7588..8889
                     /locus_tag="JNZ73_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY8733420.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9005..9343
                     /gene="cutA"
                     /locus_tag="JNZ73_003238"
     CDS             9005..9343
                     /gene="cutA"
                     /locus_tag="JNZ73_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HAY8733421.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9319..11016
                     /locus_tag="JNZ73_003239"
     CDS             9319..11016
                     /locus_tag="JNZ73_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAY8733422.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTIDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11053..11628
                     /locus_tag="JNZ73_003240"
     CDS             11053..11628
                     /locus_tag="JNZ73_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8733423.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11735..11810
                     /locus_tag="JNZ73_003241"
     tRNA            11735..11810
                     /locus_tag="JNZ73_003241"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11768..11770,aa:Phe,seq:gaa)
BASE COUNT         2926 a         3137 c         2987 g         3103 t
ORIGIN      
        1 tttctgttta tatagagtga gcacggctat ttatgttatt ttaacaacta ttactaccaa
       61 gcccatttat ctggcttata aattaaaatt aattatttaa ttgagaaatg gttagggaga
      121 acctacatgg ccataagcat caagggtgtt aataccggtg ttattcgcaa gagtaataat
      181 ttcattgcac tgccgctgaa aattaaagaa cctcgcaata aagaatcgtt gttctttatg
      241 tctgtcatgg aactgcgcga cctgctgatt gcgctggaaa gtcgtctgca tcaaaagcat
      301 aaactggatg ctgctgcacg ccttcagtat gagcaagctc gcgataaagt cattaagaaa
      361 atggcagaaa acatcccaga aattctggtt gatgagctta aaaatgccga tattaatcgt
      421 cgggttaaca cgctggaact taccgataat caaggcgaaa atcttacctt cgtattaacg
      481 ctacatgacg gtagcaaatg tgagttagtc gtcaatgagt tgcagattga aatgctggcg
      541 cgagcaatca ttcatgcaat taataatgcg gaaatgcgtg aactggcatt acgtattacc
      601 tcactgttag atttcctgcc gttatacgac gttgactgcc aggacaatgg caatctggaa
      661 tatgacacct attcccaacc ggaatggaaa cataacttgt tcgaccatta tctggcggtg
      721 ctttaccgct ttaaagatga aagtggcaaa gaacaattta gcggcgcagt ggtgaaaacg
      781 cgtgaagcaa cgccgggtaa agaaatagag gcgatcactc gccgaatgct tgatttcagc
      841 ccacgactga aaaaactcgc gggtgtgcca tgtcaggtct atgctcgcac cgtagcggcg
      901 aataacgccc aaccgctaac ccaggatcaa tgcttacgcg cgctgcatca cttacgtgta
      961 caatccacca gcaaaactgc accacaggcg aaataatctc atctttgcta atgcgaggct
     1021 ggagccatcc agcctgaaat gactgataac gtgatcgttg gtcatgccgg atgcggcgta
     1081 aacgccttat ccggcctaca aattctcaag cattcaatca gttgcaatgc ctgctgtagg
     1141 cctgataagc gaagcgcatc aggcaataat acgtttgtca cccagcctct ggatcacatc
     1201 agttcgggca cttataaacc tgaccgataa tctggctatc cgtcgggaca aaactggaca
     1261 acattccctg cgacgggctg ctgatgccat aaatcacgtt accgcccatt gcagccgcct
     1321 ggttgcgcag atcgtttgct gcgccgcgca tagaaccgcc ctcttctccg tgttgcccgg
     1381 aaagccagtt gctttgctta cctgtcgcag taccaatcag ctggcactct gcgcctggct
     1441 gctcgtccac aatgcgtaca ctctgaccgg cagcactcaa ttcgttgctg gagctacaac
     1501 ccgccatcag tagcgcggca ccgacaatcc ctgctaagta ttttacgtgc atgttattcc
     1561 ccataatcaa tgagctggac gccactcgcg tcgtccgtgt ctgaatctta tactagaaag
     1621 atgaccaaaa gaaaaacccc cagacatttc tgcccggggg tttgtttatt tctgcgaagt
     1681 gcagggcaat tacatcatgc cgcccatgcc acccatgccg cccataccgc cagcagcgcc
     1741 taagtcagct gcatcgtttt tcggcaggtc ggtaaccatg cattcggtgg tgatcatcag
     1801 gccagccaca gaagctgcgt actgcagagc agaacgggtt actttggttg ggtccaggat
     1861 acccatgtcg atcatgttgc cgtattcttc ggttgctgcg ttgtaaccgt agttgccgtc
     1921 gccgccttta acggtgttag caacaacaga cggttcttcg ccgcagttca ggacgatctg
     1981 acgcagcgga gcttccattg cacgcagtgc aactttgata cccacgttct ggtcttcgtt
     2041 ctgaccacgc aggtcagcca gtttagacgc tacgcggatc agcgcaacac caccaccagc
     2101 aaccacgcct tcttctaccg cagcacgggt cgcgtgcagg gcatcttcaa cgcgtgcttt
     2161 tttctctttc atttcaactt cggtagcagc acctactttg ataactgcaa cgccgcctgc
     2221 cagtttcgct acgcgctcct gcagtttttc acggtcgtag tcagaagttg cttcttcaat
     2281 ctgctgacgg atctgagcaa cacggccctg gattgcagct tcttcaccca cgccatcgat
     2341 gatagtggtg gtgtctttgt tgatcacaac acgtttagcc tgacccaggt cttccagggt
     2401 tgctttttcc agctccatac cgatctcttc agagattacg gtaccgccag tcagggttgc
     2461 gatatcctgc agcatagctt tacgacgatc gccgaagccc ggagctttaa ccgcagcaac
     2521 tttcacgatg ccacgcatgg tgttaacaac cagagttgct agcgcttcgc cttcaacatc
     2581 ttcagcgatg atcagcagcg gtttgcctgc tttggcaaca gcttccagaa ccggcagcat
     2641 ttcgcggatg ttggagattt tcttgtcagc cagcaggatg aacgggcttt ccagttctac
     2701 tgcgccagtt tccggcttgt tgatgaagta aggagacagg tagccacggt cgaactgcat
     2761 accttcaacc acgtccagtt cgtcctgcag accggtaccg tcttcaacgg tgataacgcc
     2821 ttctttaccg actttgtcca tcgcttcagc gatcagttta cctacggttt cgtcggagtt
     2881 agcggagatg gtaccaacct gagcaatcgc tttagaatca gagcacggta cggacagcgc
     2941 tttcagttct tcaactgcag cggtaaccgc tttgtcgata ccacgtttca ggtccatcgg
     3001 gttcatgccc gcagcaacag ctttcagacc ttcagtgatg atagcctgag ccagtacggt
     3061 tgcagtggtg gtaccgtcgc ctgcagcgtc gttcgctttg gaggcaactt ctttcaccat
     3121 ctgcgcaccc atattttcga acttgtcttc cagttcgatt tcacgagcaa cggaaacacc
     3181 atctttggtg atggtcggtg caccgaaaga tttatccaga actacgttac ggccttttgg
     3241 accgagggta actttcactg catctgccag tacgtttacg ccgcgcagca ttttcacacg
     3301 agcgtcgtta ccgaatttta cgtctttagc tgccattatc tttattcctt aaattcgtat
     3361 gttcagtgtc gtgcgcggat tacgcttcaa caattgccag aatgtcgctt tcggacatga
     3421 tcaacacttc ttcattgtcg atcttctcgg atttcacacc gtagccatcg ttgaaaataa
     3481 cgatgtcgcc aactttcacg tccagcggct tcacttcgcc attttcaagg atacggccat
     3541 tgccgacagc cagcacttcg ccgcgggtgg atttagccgc tgcagagccg gtcagaacga
     3601 tgccgccagc agatttagtt tcaacttctt tacgcttgac gatcacgcga tcatgcaatg
     3661 gacgaatatt cattgataac tctcctttga gaaagtccgt atctgttatg ggtgacgccg
     3721 gagcttacgt ggtttcccgg ctggtgacca gagaaatggg gatgaggctt cgccccttca
     3781 agggggaaaa agaaaaaaaa ttctgaacag cacaaaatcg ggtgaaaacc ctgattcacc
     3841 tcacatttca tcgcaatttc ttcatcgccg tcataagcga atctgattgt gctaccatcg
     3901 aaaatctacg cagttgccca aaatttgggc gcaatcggat atcaacccat gagtggactc
     3961 aaacaagaac tggggctggc ccagggcatt ggcctgctat cgacgtcatt attaggcact
     4021 ggcgtgtttg ccgttcctgc gttagctgca ctggtagcag gcaataacag cctgtgggcg
     4081 tggcccgttt tgattatctt agtgttcccg attgcgattg tgtttgcgat tctgggtcgc
     4141 cactatccca gcgcaggcgg cgtcgcgcac ttcgtcggta tggcgtttgg ttcgcggctt
     4201 gagcgagtca ccggctggct gtttttatcg gtcattcccg tgggtttgcc tgccgcacta
     4261 caaattgccg ccggattcgg ccaggcaatg tttggctggc atagctggca actgttgttg
     4321 gcagaactcg gtacgctggc gttggtgtgg tatatcggta ctctcggtgc cagttccagt
     4381 gctaatctac aaaccgttat tgccggactt atcgtcgcac tgattgtcgc tatctggtgg
     4441 gcgggcgata tcaaacctgc gagtatcccc ttccccgcgc caggaaatat cgaacttacc
     4501 gggttatttg ctgcgttatc agtgatgttc tggtgttttg tcggtctgga agcatttgcc
     4561 catcttgcct cggaatttaa aaatccagag cgtgattttc ctcgtgcttt gatgattggt
     4621 ctgctgctgg caggattagt ctactggggc tgtacggtag tcgtcttaca cttcgacgcc
     4681 tatggtgaac aaatggcggc ggcagcatcg cttccaaaaa ttgtagtgca gttgttcggt
     4741 gtaggagcgt tatggattgc ctgcgtaatt ggctatctgg cctgctttgc cagtcttaac
     4801 atttatatac agagcttcgc ccgcctggtc tggtcgcagg cgcaacataa tcctgaccac
     4861 tacctggcac gcctctcttc tcgccatatc ccgaataatg ccctcaatgc ggtgcttggc
     4921 tgctgcgtgg tgagcacgtt ggtaattcat gccttagaga tcaatctgga ggctcttatt
     4981 atttatgcca atggcatctt tattatgatt tatctgttat gcatgctggc aggctgtaaa
     5041 ttattgcaag gtcgttatcg actactggcg gtggttggcg ggctgttatg cgttctgtta
     5101 ctggcaatgg tcggctggaa aagtctctat gcgctgatca tgctggtggg gttatggctg
     5161 tttctgccaa aacgaaaaac gccggaaaat ggcataacca cataatccgg cgtttcgaca
     5221 ttaatcctgg cgatcgtctt tatgatcaag gcggtcgcgg tcatcatcct ttcgctgata
     5281 ctcaccatca aaagtattac cgccaccggt cccggcgcta aaaccgccgc ctggcatgcg
     5341 agaaaagcgc aaatgtggca tcaacttcac tgtcagatgc ttttgcaccg gcggcaataa
     5401 aagcagaaga ccgaggaagt cggtgaaaaa gcccggtaat aaaagcagca aaccggcaat
     5461 aatcagcgaa acacttttaa tcatctccgc cgcagggttt tcacccgccg ccattttttg
     5521 ctgcatcagc acgaaattct taaagccctg gttacgtacc agtgacatac cgataactga
     5581 cgtgaatata accagcacga gggtcagcaa tacccccaat acatgggcaa cctgaataaa
     5641 gattgaaatc tcaatataga cataaaggaa aatggcaata aaaggtaacc agcgcaaagg
     5701 tttctcctgt aatagcagcc ggttaaaccc ggctacctga atgggttgcg aatcgcgttc
     5761 agcttatatt gtggtcatta gcaaaatttc aagatgtttg cgcaactatt tttggtagta
     5821 atcccaaagc ggtgatctat ttcacaaatt aataattaag gggtaaaaac cgacacttaa
     5881 agtgatccag attacggtag aaatcctcaa gcagcatatg atctcgggta ttcggtcgat
     5941 gcaggggata atcgtcggtc gaaaaacatt cgaaaccaca tatattctgt gtgtttaaag
     6001 caaatcattg gcagcttgaa aaagaaggtt cacatgtcaa acaacattcg tatcgaagaa
     6061 gatctgttgg gtaccaggga agttccagct gatgcctact atggtgttca cactctgaga
     6121 gcgattgaaa acttctatat cagcaacaac aaaatcagtg atattcctga atttgttcgc
     6181 ggtatggtaa tggttaaaaa agccgcagct atggcaaaca aagagctgca aaccattcct
     6241 aaaagtgtag cgaatgccat cattgccgca tgtgatgaag tcctgaacaa cggaaaatgc
     6301 atggatcagt tcccggtaga cgtctaccag ggcggcgcag gtacttccgt aaacatgaac
     6361 accaacgaag tgctggccaa tatcggtctg gaactgatgg gtcaccagaa aggtgaatat
     6421 cagtacctga acccgaacga ccatgttaac aaatgtcagt ccactaacga cgcctacccg
     6481 accggtttcc gtatcgcagt ttactcttcc ctgattaagc tggtagatgc gattaaccaa
     6541 ctgcgtgaag gctttgaacg taaggctgtc gaattccagg acatcctgaa aatgggtcgt
     6601 acccagctgc aggacgcagt accgatgacc ctcggtcagg aattccgcgc tttcagcatc
     6661 ctgctgaaag aagaagtgaa aaacatccaa cgtaccgctg aactgctgct ggaagttaac
     6721 cttggcgcaa cagcaatcgg tactggtctg aacacgccga aagagtactc tccgctggca
     6781 gtgaaaaaac tggctgaagt cactggcttc ccatgcgtac cggctgaaga cctgatcgaa
     6841 gcgacctctg attgcggcgc ttatgttatg gttcacggcg cgctgaaacg cctggctgtg
     6901 aagatgtcca aaatctgtaa cgacctgcgc ttgctctctt ctggtccacg tgccggcctg
     6961 aacgagatca acctgccgga actgcaggcg ggctcttcca tcatgccagc taaagtaaac
     7021 ccggttgttc cggaagtggt taaccaggta tgcttcaaag tcatcggtaa cgacaccact
     7081 gttaccatgg cagcagaagc aggtcagctg cagttgaacg ttatggaacc ggtcattggc
     7141 caggccatgt tcgaatccgt tcacattctg accaacgctt gctacaacct gctggaaaaa
     7201 tgcattaacg gcatcactgc taacaaagaa gtgtgcgaag gttacgttta caactctatc
     7261 ggtatcgtta cttacctgaa cccgttcatc ggtcaccaca acggtgacat cgtgggtaaa
     7321 atctgtgccg aaaccggtaa gagtgtacgt gaagtcgttc tggaacgcgg tctgttgact
     7381 gaagcggaac ttgacgatat tttctccgta cagaatctga tgcacccggc ttacaaagca
     7441 aaacgctata ctgatgaaag cgaacagtaa tcgtacaggg tagtacaaat aaaaaaggca
     7501 cgtcagatga cgtgcctttt ttcttgtgag cagtaactta aaaataacaa tctaatatca
     7561 acttgttaaa aaacaaggaa ggctaatatg ctagttgtag aactcatcat agttttgctg
     7621 gcgattttct tgggcgccag attgggggga ataggtattg gttttgcagg cggattgggg
     7681 gtgctggttc ttgccgctat tggcgttaaa cccggtaaca tcccgttcga tgtcatttcc
     7741 attatcatgg cggttatcgc cgctatttct gccatgcagg ttgctggcgg tctggactat
     7801 ctggttcatc agacagaaaa gctgctgcgc cgtaacccga aatacatcac gatcctcgca
     7861 ccgatcgtga cctatttcct gactatcttt gctggtactg gcaacatctc tctggcgaca
     7921 ctgccagtta tcgctgaagt tgcgaaggaa caaggcgtta aaccttgccg tccgctctct
     7981 actgcagtgg tatccgcgca gattgcgatc accgcatcgc caatctcagc ggcagtggtt
     8041 tacatgtctt ccgtgatgga aggtcatggc atcagctacc tccatctgct ctccgtggtc
     8101 atcccgtcca ccctgctggc ggttctggtg atgtccttcc tggtcactat gctgttcaac
     8161 tccaaactct ctgacgatcc gatttatcgc aagcgtctgg aagagggctt ggttgaactg
     8221 cgcggtgaaa agcagattga aatcaaatcc ggtgcaaaaa cgtccgtctg gctgttcctg
     8281 ctgggcgtag ttggcgtggt tatctatgca atcatcaaca gcccaagcat gggtctggtt
     8341 gaaaaaccgc tgatgaacac caccaacgca atcctgatca tcatgctcag cgttgcaact
     8401 ctgaccaccg ttatctgtaa agtcgatacc gacaacatcc tcaactccag caccttcaaa
     8461 gcaggtatga gcgcctgtat ttgtatcctg ggtgttgcgt ggctgggcga tactttcgtt
     8521 tccaacaaca tcgactggat caaagatacc gctggtgaag tgattcaggg tcatccgtgg
     8581 ctgctggccg tcatcttctt ctttgcttct gctctgctgt actctcaggc tgcaaccgca
     8641 aaagcactga tgccgatggc tctggcactg aacgtttcac cgctgaccgc tgttgcttct
     8701 ttcgctgcgg tgtctggtct gttcattctg ccgacctacc cgacgctggt tgctgcggta
     8761 cagatggatg acacgggtac tacccgtatc ggtaaattcg tcttcaacca tccgttcttc
     8821 atcccgggta ctctgggtgt tgccctggcc gtttgcttcg gcttcgtgct gggtagcttc
     8881 atgctgtaat gatcaatcgc ggggcgttca cgccccgctt tctttcccgc cgactaacat
     8941 ccttcccccg tccgttgtat agtgacctct ctcttgcggt tccatctgtt cttgcgaggt
     9001 gtttatgctt gatgaaaaaa gttcgaatac cgcgtctgtc gtggtgctat gtaccgcacc
     9061 ggatgaagcg acagcccagg atttagccgc caaagtgctg gcggaaaaac tggcggcctg
     9121 cgcgaccttg atccccggcg ctacctctct ctattactgg gaaggtaagc tggagcaaga
     9181 atacgaagtg cagatgattt taaaaactac cgtatctcac cagcaggcac tgctggaatg
     9241 cctgaagtct catcatccat atcaaacccc ggaacttctg gttttacctg ttacacacgg
     9301 agacacagat tacctctcat ggctcaacgc atctttacgc tgattctgct actttgcagc
     9361 acttccgttt ttgccggatt attcgacgcg ccgggacgtt cacaatttgt ccccgcggat
     9421 caagcctttg cttttgattt tcagcaaaac caacatgacc ttaatctgac ctggcagatc
     9481 aaagacggtt actacctcta ccgtaaacag atccgcatta cgccggaaca cgcgaaaatt
     9541 gccgacgtgc agctgccgca aggcgtctgg catgaagatg agttttacgg caaaagcgag
     9601 atttaccgcg atcggctgac gcttcccgta accatcaacc aggcgagtgc gggagcgacg
     9661 ttaactgtca cctaccaggg ctgtgctgat gccggtttct gttatccgcc agaaaccaaa
     9721 accgttccgt taagcgaagt ggtcgccaac aacgcagcgt cacagcctgt gtctgtttcg
     9781 cagcaagagc agcacaccgc gcaattgccc ttttccgcgc tctgggcgtt gttgatcggt
     9841 attggtatcg cctttacgcc atgcgtgctg ccaatgtacc cactgatttc tggcatcgtg
     9901 ctgggtggta aacagcggct ctccactgcc agagcattgt tgctgacctt tatttatgtg
     9961 caggggatgg cactgactta cacggcgctg ggtctggtag ttgccgccgc agggttacag
    10021 ttccaggcgg cgctacagca cccatacgtg ctcattggcc tcgccatcgt ctttaccttg
    10081 ctggcgatgt caatgtttgg cttgtttact ctacaactcc cctcttcgct gcaaacacgt
    10141 ctcacgttga tgagcaatcg ccaacagggc ggctcacctg gcggtgtgtt tgttatgggg
    10201 gcgattgccg gactgatctg ttcaccatgc accaccgcac cgcttagcgc gattctgctg
    10261 tatatcgccc aaagcgggaa catgtggctg ggcggcggca cgctttatct ctatgcgttg
    10321 ggcatgggcc tgccgctgat gctaattacc gtctttggta accgcttgct gccgaaaagc
    10381 ggcccgtgga tggaacaagt caaaaccgcg tttggttttg tgatcctcgc actgccggtc
    10441 ttcctgctgg agcgagtgat tggtgatgta tggggattac gcttgtggtc ggcgctgggt
    10501 gtcgcattct ttggctgggc ctttatcacc agcctacagg ctaaacgcgg ctggatgcgc
    10561 gtggtgcaaa taatcctgct ggcagcggca ttggttagcg tgcgcccact tcaggattgg
    10621 gcatttggtg cgacgcatac cgcgcaaact cagacgcatc tcaactttac acaaatcaaa
    10681 acgatagatg agttaaatca ggcgctcgtt gaagccaaag gcaaaccggt gatgttagat
    10741 ctttatgccg actggtgcgt cgcctgtaaa gagtttgaga aatacacctt cagcgacccg
    10801 caggtgcaaa aagcgttagc agacacggtc ttacttcagg ccaacgtcac ggccaacgac
    10861 gcacaagatg tggcgctgtt aaagcatctt aatgtccttg gcctaccgac aattctcttt
    10921 tttgacggac aaggccagga gcatccacaa gcacgcgtca cgggctttat ggatgctgaa
    10981 accttcagcg cacatttgcg cgatcgccaa ccgtgaacga cacttgcagt gggaaagacg
    11041 gaggaaaata gcgtgcaacg tgaagatgta ctgggagaag ccctgaaatt attagaatta
    11101 caagggattg ccaacaccac gctggagatg gttgctgaac gtgtggatta tccactggac
    11161 gagctacgcc gcttctggcc agacaaagag gcaatcctct acgatgcgct gcgttatctt
    11221 agccaacaga tagatgtctg gcgtcgtcag ctgatgctgg acgaaacgca aaccgccgaa
    11281 caaaagctgc tggcacgtta tcaggcgtta tcggagtgcg ttaaaaacaa ccgctatccg
    11341 ggctgtctat ttatcgctgc ctgtacgttt tatcccgatc ctggccaccc tattcatcaa
    11401 ctggccgatc agcaaaaaag cgcggcctac gatttcaccc acgaactgtt aaccacgctg
    11461 gaagttgacg atccggcgat ggtagcaaag cagatggaac tggtgctgga aggctgttta
    11521 agccgaatgc tggtgaatcg tagccaggcg gatgtcgaca ctgcacatcg gctggcggaa
    11581 gatatcctgc gcttcgcccg ctgccgccag ggtggtgcat tgacctgaca gaaacacaga
    11641 aaagaagcga tttgccgcaa tcttaagcag ttgaatcgct tttactgaaa ttaggttgac
    11701 gagatgtgca gattacggtt taatgcgccc cgttgcccgg atagctcagt cggtagagca
    11761 ggggattgaa aatccccgtg tccttggttc gattccgagt ccgggcacca aattcatatc
    11821 aacggacctc cacggaggtc cgtttttcgt ttcaggacgc cacgatttaa gcgttctgcc
    11881 tccaaatcaa ttctaccgaa ctcaaccaga ttctccccac atcaccagca atttgcgagc
    11941 atatcccaat ttgggaaaat ttgttcctga gctatagcac taactgacgt gaaatgtcgt
    12001 gcggccccgt gatgctgttg aacgtcaaat gacgtcatca ggagcgtaac gtatccataa
    12061 agcacaacac cggtctgaac gccgactgat gagattttct gaatgagaac aaagagaaat
    12121 gtgtcagtcc gcttgctcat gcaaagacta aca
//
LOCUS       DADMGC010000118        11928 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.118, whole genome shotgun sequence.
ACCESSION   DADMGC010000118 DADMGC010000000
VERSION     DADMGC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.118"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(1..2129)
                     /locus_tag="JNZ73_003242"
                     /pseudo
     CDS             complement(1..2129)
                     /locus_tag="JNZ73_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            complement(2273..2896)
                     /locus_tag="JNZ73_003243"
     CDS             complement(2273..2896)
                     /locus_tag="JNZ73_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HAY8733424.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVINH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2893..4581)
                     /locus_tag="JNZ73_003244"
     CDS             complement(2893..4581)
                     /locus_tag="JNZ73_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HAY8733425.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRLRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAVGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWCREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(4730..7357)
                     /gene="gyrA"
                     /locus_tag="JNZ73_003245"
     CDS             complement(4730..7357)
                     /gene="gyrA"
                     /locus_tag="JNZ73_003245"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HAY8733426.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYGTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7504..8226
                     /locus_tag="JNZ73_003246"
     CDS             7504..8226
                     /locus_tag="JNZ73_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HAY8733427.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8354..11928)
                     /gene="yfaL"
                     /locus_tag="JNZ73_003247"
                     /pseudo
     CDS             complement(8354..11928)
                     /gene="yfaL"
                     /locus_tag="JNZ73_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2767 a         3309 c         2939 g         2913 t
ORIGIN      
        1 tcgacattga ccagttcacc aggctggtaa tgggttttgt ccgttttcac ccggatatca
       61 agctgaggaa cggcaacttt gatcccggcg ttctgaaaac tgtactgacc attacgggta
      121 tacagcaccg aaaaagtgat gttaggcgca aaggaattgc tcactggaac ccgggcttca
      181 tacagggtat cgtttaaacg ttgtagcgtt agccagtttg ccggatgcga aagcagcgac
      241 tgctgttcca cgcgatcgcg ttccagcgtc aataacgctt catcaattgg ctccgggaaa
      301 gtaatcagca tcttcgcggt ttcgcctggc tggtacagcg ttttatccgc cacgatatct
      361 accgtaccag tatgcgccgt gctgccctta ccgctgacgg catgacttaa cccagcgaga
      421 attaagccgt ctttatcgcg taatgtcaga ttgtagttgc caggtttagc gaaattgacg
      481 gtaaaggatt tgccgcctga cggtagctct ccgctatggc tcgtgcggtc ttcgagacgc
      541 aaccattcat acgtaacagg aacctgtttt gaagattcca gcgcggcata acggaacaca
      601 accgactcgc cgctattact gtattgtgcg gcggtactta aagagtaatg cgccaggccg
      661 cgttcaatga ggagctcttt ggtggtggtg acgcgatacg ccgcgccgtc actggcggag
      721 acggttaaca aatagcggct cggtttatcg gcggcgggga gatttaacgc cacataaccg
      781 ctggcgtcgg acaccgtttc gctgccttcc agcgacacgg ggaaacgtcc ggcataacgc
      841 aaatcgttac cgaccattga taattgctga gcgcgcaaac ttaactgcac gcgggcattt
      901 tttaccggct cgccatccgg gtagagcagt tgcagtttgc cgctgaccgc ttcgccagtt
      961 ttgaactctt ttttgtcgag agctaaacca atctcgaaat gtggcttgat gtagtttgcc
     1021 acgcgaaaac tgctgctata gacctgattg cggtaagcaa gacgtaactc ataacctccg
     1081 gcaacggcat tttctggcag gcggaaactt ccctgcccgc cattgcgcgc atccagcgtg
     1141 acatcgacgg tttgcaacag actgccgtta gcgtccagca ccgaaagctt cgccggggcg
     1201 ctgacgatgg gggatgaatg caacggatcg cggaactcgc ggcccatcac tttaacatcg
     1261 acacgatcgc ctgcgcgata tagcgggcga tcggtaaaaa tatacaagcg ggtgttgtag
     1321 atttcgcttt cgtagaagaa gttctcggag acaaaaacgc cgccttcagc atccttaccc
     1381 agaatgtatg aacgttctgg cgatatatgt tgtaactgca aagtaccgct gtcatcggtc
     1441 accccgcggg tcatcacgcc aagaccgtca gtccacaaga tctctgagcc gggcttcgct
     1501 tcaccctgtt ttttacccgc ggtccatacc agaagctcgt tacctgacac tttgctaagc
     1561 gccacggtat cggaaacaaa caccaccgtc gtcgcccgat acccacctac catcgcctcg
     1621 acgaggtaca gccccggctc ttgttggccg agaggaatat aaatattacc cggctgcggc
     1681 gagatgaaat tgctggatgc gccttccagt tttatccctt gctgcggctc gaccggttta
     1741 gcctgccata gtggataacg gaactgttcc accaagggat attttttcag cggggaaaac
     1801 tggttgttct gtacataacg ggaaggttta ataatggcat tgccgagatg taattcgggt
     1861 aatgcctgag tcacattctg ccgtgactga gaagagaaag tacgctgcat cacgcggcga
     1921 gatttgccgt accagttatc ccacagctag gtcagcgtat tgttcagccc gtcgcccaga
     1981 tattgcggtt gcaccacaat gcgatgcagg tttttctgct ggcgcaaaaa tgccatcggg
     2041 tcaggaatac gatacaggcg aacgtccacg ccgccgtact cttccatctg ataacgccga
     2101 taatcacgcc ccggcgcttc cagtcgcact ttcgcctctt cactgctgct aaaactgctg
     2161 tcagcaagca aaaagaatgt tcccccggcg ggcggcgcat agttactgga aggaagcgaa
     2221 tcatcagctt tagcaagccc tgttcccaca agagacaggg acaagcacgc tgctatcgcg
     2281 ccagaaaatt taaacgataa atgccaatga aattgggatt ggaatcgttg ggtatccatc
     2341 gggtgtcctt ccatgtcata agttgttgca gactgactgc gcgcattccg ttgtcagttt
     2401 tcgtggcgct tccggtgtgg ttgatgacgt aacgccccat ccagaccatt aagtgctggg
     2461 catcgccctg atcgaaaaaa atcatatcgc caggcagcgc ctggtttatg tcttggccaa
     2521 taaactggct gttgtactga atcaaattaa tcgcggtcac gtaggggccg tttttcccgc
     2581 tcccctgatt ccagttttgc gccagttgac gctgttcagg tgttagcgtc atctctggcg
     2641 gcaaatactg gctagataaa ccgttacttt ttagccattt actgtcgtga actttcagcg
     2701 tctcgttcgc cgcaaatcgc accaggcccg cacaatcctg ctgataccag cgtggacttg
     2761 gcccctggcg gagctgttct tgtgcaatgc gtacaaacca ggcgcgaaac agcctggatt
     2821 gttcgacatt cagcatttcg ctatgggcaa caacacaaca cagccagcaa atcagcgcca
     2881 gcagcccgtg tctcatagcg gctgccaggt tatgggtagc cactgccagg cgacaccggg
     2941 ttccatctgg gccagcttca tgacataacg cggttgttga gataaagcgt ccagcttcgg
     3001 cattaataaa gtttgtgcgg cgttataaaa aaccggttcg agattcttcg gcagactggt
     3061 caacgtttcg ttacgcagca gtttcgccac gccttgtgga ttgatataga gcggaacgat
     3121 gccatcagtg ggtattacat ccaccattgc cgggcgggtt ttattcagtg tttgcagggc
     3181 gttattaacc agcgtgtcat cgagggagaa aagcagcgtt ttgttttgca tcgccagcga
     3241 cacacggaaa aaataatccg acattaattg atcgggttgc gccgcctgcg ctttcggata
     3301 ctgaccgtat cgggaactca cttcgcgaca ccagatttgc gcttcgccct gctgagtctg
     3361 gcttaccggc aaaacacctt ctggcgcttt gctttcgtgc gcaccaatat tttgcgtaaa
     3421 cagtttccct ggcaattgcg cctgttcggc agtgccatca aactgaccga caaacagcgg
     3481 ggtttgcaat tttgagtctt cataccagca cagccccacg gcaccgtcta acgccccatt
     3541 cagcttgtcg ttttcctggc tgatgtgcga aagcatctct tcggcaatac cgtgtgaata
     3601 cggcaccgcc acacagaagc tggctccggc aggcatactg ttccagaccg gcgtaaaatc
     3661 gaaactggca tctacgctgg cggattcatc atttaacgcc acaaaactgt gccagccgtc
     3721 gttacccatt tcgaagcgta cgccagcaaa agaaggcatt aaccgctggt agccaaaacc
     3781 cagcaacctg gcgctggcca cgatgcgcag gcgtactggc gttttttcgg ctgtacgctc
     3841 ttccaggcca aagctggctt gccagcgttt tttaccgctc aacaaatcac ttgcgatcgc
     3901 cgtggcttcg gtatcctgct gatcatcttt aaacaacata tccgtactgg aaaacaccag
     3961 catcttgtcc tgatatgtcg cgaacatcag ggcgttattg ccgttatagc gcaactgata
     4021 aacaggaata gtttcactat ttatcttgat gctactgatt tccgttttgc ttaactggct
     4081 gtcgctggta gcggcaaaca acaatggctc cagcagtttg cttaaaccac tgcgctggat
     4141 cagcaccatg taatgtgaaa gatggccttg tttatcgtgc catagcgccg cctgcgcggg
     4201 ctgatctaag agtgacgaaa agagcttatc tttcagcgtg agatcgtgtt catagacaat
     4261 gcggcgaatg ctgccttcaa tgcccagacg atctgcatga ttctgataat agaaaacgaa
     4321 atcctcgctc agaacatcgt ggagaaacgg aatggtgagg agatctttgg gaagctggct
     4381 cagagagtcg ctgtctagaa agaggtccgg ctcattgaga tcgatttgca gattgttgtg
     4441 caccaccagc ggcgacaacg ttttttctgg cccactgcca gcatattgca acgcccagac
     4501 gccagcggaa agcagtgcta ttgcgccaaa acctacaaga ccatagaacc gccagccttt
     4561 cgcctttttt tcaccactca ttgccacatt ccttgtgtat agccagccat tttttacggg
     4621 cacagccaaa ctttaccgcg ccctaatacg acaaaagccc agactttgca gcctggactt
     4681 ttcaattcaa acaagggaga tagctccctt ttggcatgaa gaagtaaaat tattcttctt
     4741 ctggctcgtc gtcaacgtcc acttccggag caatttcatc atccccttcc gcggcactgc
     4801 cgtcgatggt atccagatct tcctcgtcaa ccggttcagc aacacgctgc agacccacta
     4861 cgttttcatc ttccgcagta cggatgagga tcacgccctg ggtgttacgg cccacgatgc
     4921 tgatttctga aacgcgagta cgtaccagcg taccggcatc ggtgatcatc atgatctggt
     4981 cgcagtcatc tacctgcacc gcgccaacca ctaaaccgtt acgttcggta accttgatgg
     5041 agataacccc tttcgtcgca cgcgacttgg ttgggtattc cgccactgca gtacgtttac
     5101 cgtaaccgtt ttgcgttgcg gtgaggattg cgccatcgcc acgaggcacg atcagagaga
     5161 cgactttatc gccttcacct aagcgaatac cgcgcacacc agtggtattg cagcccatcg
     5221 cacggacaga agactcttta aagcgcacca ctttaccttc ggcggagaac agcattactt
     5281 catcttcgcc actggtcaga tcaacgccga tcagctcatc gccgtcaacc agtttgatcg
     5341 ccactttacc ggcggtacgc agacggttga attcggtgag gacggttttc ttcacagtac
     5401 cgttagcggt cgccatgaag actttcacgc cttcttcaaa ctcggtcacc ggcaggatcg
     5461 cggtgatacg ttcgtcctgt tccagcggta gcaggttgac gatcggacga ccgcgcgcgc
     5521 cacgagtggc ttccggcaac tggtaaactt tcatcgaata gacgcgacca cggctggaga
     5581 agcacagaat atggtcgtgc gtgttcgcca ccagcagtcg gtcgataaag tcttcttctt
     5641 taatacgtgc ggcagattta cctttcccgc cacgacgctg cgcttcgtat tcagaaagcg
     5701 gctgatattt aacgtagccc tggtgagaga gcgtcacgac cacatcttcc tgggtgatca
     5761 gatcttccag gttgatgtct gcgctgttgg cggtgatttc agtacgacgt ttgtcaccga
     5821 actgttcacg aaccagctcc agctcttcgc ggatcacttc catcagacga tcggcgctac
     5881 caagaatacg caacagttcc gcgatctgat ccagcagctc tttgtattcg tcgagcagtt
     5941 tttcgtgctc aaggccggtc agtttctgca aacgcagatc cagaatcgcc tgagcttgct
     6001 gttcggtcag gtagtacaga ccatcacgca cgccgaactc tggctccagc cattccggac
     6061 gcgcagcatc gtcgccagca cgttccaaca tggcggcaac gttgcccagc tgccacggat
     6121 tagcaaccag cgcagttttc gcttctgcag gcgtcggcgc atgacggatc agttcgatga
     6181 tcgggtcaat gttcgccagc gccacggcta atgcttcaag gatatgagca cgatcgcgag
     6241 ctttacgcag ttcgaaaata gtacgacggg tcaccacttc acggcggtga cgaacaaacg
     6301 ccgcgatgat gtctttcagg ttcatgatct tcggctgacc atggtgcaat gccaccatgt
     6361 tgataccgaa agaaacctgc aactgggtct gggagtagag gttgttgagc acaacttcac
     6421 cgaccgcatc gcgtttcact tcaatcacga tgcgcatacc gtctttgtca gactcgtcac
     6481 gcagcgcgct gatgccttcc acgcgttttt cttttaccag ttccgcaatc ttctcgatca
     6541 ggcgcgcttt gtttacctga tacggaattt cgtggacgat aatggtttca cgtccggttt
     6601 tggcgtcaac ttccacttca gcacgggcgc ggatatatac cttgccgcga ccggtacggt
     6661 aagcttcttc aataccgcga cgaccgttaa tgattgccgc cgtcgggaag tccggccccg
     6721 ggatgtgttc catcagccct tcaatgctga tgtcttcatc atcgatatac gccagacaac
     6781 cgttgatgac ttccgtcagg ttgtgcggcg ggatgttggt tgccatacct acggcgatac
     6841 cggaagaacc gttcaccagc aggttaggaa ttttggttgg catgacgtcg ggaatttttt
     6901 ccgtgccgtc atagttatca acgaaatcga ccgtctcttt ttcgagatcg gccatcagtt
     6961 catgggcaat tttcgccaga cggatttccg tataacgcat tgccgccgca gagtcgccgt
     7021 cgatggaacc gaagttaccc tgaccgtcta ccagcatgta acgcagcgag aatggctgcg
     7081 ccatacggac gatcgtgcca taaaccgcca agtcaccatg gggatggtat ttaccgatta
     7141 cgtcaccaac gacacgggca gattttttat aggctttgtt ccagtcattg cctagtacgt
     7201 tcatggcgta aagtacgcga cggtgtaccg gcttcaggcc atctcggaca tctggcagcg
     7261 cacggccaac aatgaccgac atcgcataat ccagatagga gctctttagc tcttcctcaa
     7321 tgttgaccgg tgtaatttct ctcgcaaggt cgctcatcta accgctatcc ctctactgta
     7381 tcccggattc aaaggtcgca aattataaca cagccgcgca gtttgaggta aacctatacg
     7441 ctttattcac atccaatgcc tgatatactc gtttgtcttg ccaattacgg agtagaagtg
     7501 ccaatgaatg ccgaaaaatc gccggaaaac cataacgtag accatgaaga gatcgctaaa
     7561 tttgaagccg tcgcctcccg ctggtgggat cttgaaggtg agttcaaacc gttgcaccgc
     7621 attaacccgc tgcgtctggg ctatattgcc gagcgtgctg gcggtttatt tggcaaaaag
     7681 gtgctcgatg tcggttgtgg cggcggcatt ctggccgaga gtatggcgcg cgaaggcgcg
     7741 acggtgaccg gtctggatat gggctttgag ccattgcagg tggcaaagct gcacgcgctg
     7801 gaaagcggca ttcaggtgga ttacgtgcag gaaaccgtgg aagagcacgc ggcaaaacat
     7861 gccgggcagt atgatgtggt gacctgcatg gagatgctgg agcacgtacc cgatccgcag
     7921 tcagtggtca gagcctgtgc gcaactggtg aaaccaggcg gcgatgtctt tttctcgacg
     7981 cttaaccgca acggcaagtc atggctgatg gcggtggttg gcgcggaata tattttgcgc
     8041 atggtgccca aaggtacgca tgatgtgaag aagtttatta aaccggcaga gctgcttggc
     8101 tgggtggatc agaccagttt aaaagagcga catataaccg ggctacatta caacccgatc
     8161 actaattcct ttaagctcgg ccccggcgtg gatgtgaact atatgctgca cacgcagaat
     8221 aagtgaggtt gatgtttggc cgcgccaatg cctgatgcga cgcttgcagc gtcttatcag
     8281 gcctacaaat gctctccgta ggccagataa ggcgtttacg ccgcatccgg caattgtgat
     8341 ggtcagacag cgattaccat ttcaccgtca tcgacaaaaa ccctgccgtc tgggcaaaat
     8401 catcactccc tttctgccac gccacgctgc cgcgcagaga cactcgctga ctgatattgc
     8461 ccgtgactcc cacttttatt tcaccccgta gcttcaccgc atcgtcactg atagtgctgc
     8521 catcttcttc aatttccgtc gcatggggat cgtgataata attcagatct aatgttggtg
     8581 tgacaccaac cgcggtacgc cattcgctgt gtaaacccag ccgcgtctga atatcatccc
     8641 cctgcgattg tgacacgcgc gcatggttag cggcggtaaa atcatcctgc tgcacgccct
     8701 gataaatcac ctgcgcctgc ggttcaatca ccacaccacg ccccggtaac cactgatacc
     8761 cggcttccag cgaggcgata atccccgacg aatggtaacg atccacgcca tcttcatgtt
     8821 cggaaacatc attgttaaac cacgcgtatt gcagccagct atccagccag gccccctgct
     8881 tctgattacc gtgctgatac cagcttgagg tcagcccaac ggcataaccg tggttctggt
     8941 tatcggcgcg agttccggtc atattcgagc ggctgtcgcc ctgattatca ctgtagccac
     9001 caaccgcccc aagcatccac tcgccatccg tgccccagca cccgctaaac aagccgccgc
     9061 taagcagcac cgtagaagtg tcttcctgct gagccagttg ccctgccgcc gtgtaatgat
     9121 aacgtccgcc gataacacgt aaattcagcg tctgaccatc gccaccagcg tgatcgcgtc
     9181 gctccatcat aaacgcctga tttgccgccc gcaggttatt gagataaccg ccaactttgg
     9241 cattcagcac cggctggtaa gcaggcgtag gggccgggtc gggggttggg tcaggatccg
     9301 gcgtgggatc aggatcggga gtcgggtctg gctcaggtgg cgatggcggc gttacttctt
     9361 gcgatcgcag ataccagtcg ttgttatctt ccactagcgt gtagtcatac gctcccatgt
     9421 tgacgtagcc actgccagcc agactgaact gtgcattgtt ttgaaactgc gtgggatcag
     9481 ctgcgaaatc aaccacttta atgcctgtcg atgtcggctc accaatccct gtaatggagt
     9541 taaccaccac agttgtgttg ccagcagtat taccgttcat caccaattga tcgcttaccg
     9601 agtcatcgcc gtttaattcg ctgtcgaata gtagcgtacc gccgccggta taatcaccgt
     9661 ttaccgtcag cgtatcgcca gcgacgccat tttgcaggct aaccgcaccg gacgtattgg
     9721 tcagatcacc attaaccgtc acgtcagcaa gataggtcag gctgtcagtt tcaaggttgg
     9781 tattaagctc gcctgtcaac gtcacgccat cggtagcatt taccagcgtc ccatcgccgt
     9841 taaatagtga ggcattaagg gcgacggaag tatcctgccc ggtcagacgc aaaaccgtac
     9901 catcgctgat gtcgatagtg ccgctggaag tagcatcaat tgactgaata tcctgatccg
     9961 cgccagtaac gaacgttgcc ccatcaccaa cccacagtga cgaagcataa ggaaagatat
    10021 ccgcaacatc gcctttcagg gtgccttctt cgacacgtac cgccgactgc gttgtaccgc
    10081 tggcggtcag ctccagcgta cccgcccccg ttttagtaaa tgtgctaccc tcatcctgat
    10141 gctgcccgct gctgtcagcc atcagtgcgc cccactgcgt gtctaccccc gcatcaactg
    10201 ccacttcacc gtcggcgcgc atttcaatat cacgcccatg ctcagtcgtc gtagagcgag
    10261 tatcgacatt ggcagtaatc tccatcacgc tttcttgtta cttatcggta aagataacct
    10321 ggcggttata gtgggtatct ccaagctgcg agttgtcgct caccatcagc gtaccggagg
    10381 cgatttgtgt tgtgcccaaa taactattat tgtttgccag acttacctgc ccgtcgctcg
    10441 catcgaccac cacgtagacg ccatctttcc cgtcaccgcc gatatgcaaa ttgtcgccac
    10501 ctgcaagatc taccacatcc tgactgccga taaccgttcc gctgctgcca ctgacttcaa
    10561 ggccatcatt gtatgatgtg ccgctctgcc aagtggagaa atcagagaga tcgagcacac
    10621 cgccgtttaa cacgatcgac tgcggatcgt cctggagagc ggtaagatct gccgcgtcgc
    10681 cttccagcga aagcaccgca ccatcatcaa cgactatatc gccggttagc gccatcgact
    10741 gcgcccctgc cagcacgtag ctgccgtttt gcgcaatggt gagctgacca gcaccttcga
    10801 tgatgccagc aaaactgccc tgattaacag taacgttgcc accagcatcg atatttaaag
    10861 tgccattctg aaagcccgtc aatgcgtgca caaaagtttg ttgggtcgag ccaacgttta
    10921 gctcagcctg attctgatac tgatcaatac tccctatcgt cagaccgtag cagtcttgcg
    10981 gatcgtcctg gcaatgcgta tcgccgacat tcatcaggga gttgctgcga cccagggtaa
    11041 cttcaccgtt ttcaatctgc atctcaccag taaagtcatt gttatctgca ttaagtacca
    11101 gatcgccgga acctgttttg gtgattaacc cggtaccagc aatagagtca acagctccgt
    11161 cattctctgt attgccgata accagcgttt ttccgtcggc aatatcaaag gtaacttcac
    11221 ttaagccgag atacataaag ccacccgccg cagtggaagg accatctcca tagcctgctg
    11281 cgctattgtt ctcatcgact aacacgccgc cgttctggct gtagctgtca tcaacagaaa
    11341 tatcaataag atagggagcc ctcacgctat tggtatatat cgccccgcca taaccttcgg
    11401 ctgtgttatt tgtaaaggcg gtgttattta ttatcgtata acctgaagga tgcttgctgt
    11461 cgctattatt atcggtaaca tcgattgccc cgccatcgcc atcactgtaa cttgttgatg
    11521 tatatgcctg gttattgtta aaaacatcat cacttaaata gacgtcatta ttaatggtat
    11581 aaattgcgcc acctttgcca tcattagcga tattattgcg aaacatggcg ttagtgacac
    11641 gtaaatcgac ggcaccagta tcattagtac cagaagaata gattgcgcca ctatagccgc
    11701 ctgcgacgtt accggaaaaa ataacatcag taagattcag cgttgagttt tctttagcaa
    11761 atatggcccc gccgttatta tattctcctg taacggtgtt attggcaaac agagtcattc
    11821 cagtttcatt ttctggtaat agtgaaaatt ccgctccttg ttgcaaaaat acggccccac
    11881 cgctggcatt attggtcata tccgaaaaaa ccagccattg cccatcgg
//
LOCUS       DADMGC010000119        11847 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.119, whole genome shotgun sequence.
ACCESSION   DADMGC010000119 DADMGC010000000
VERSION     DADMGC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.119"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..1019
                     /locus_tag="JNZ73_003248"
     CDS             <1..1019
                     /locus_tag="JNZ73_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY8733428.1"
                     /translation="ELEEKWLDLKRMEISANAFNRYESVARTMVPKIGGGRLVSSVTK
                     EELLYIRKDLLTGYQNSTKNKAAAKGRSVVTVNYYMTTIAGMFQFAADHGYLEANPFQ
                     GIKPLKRARAEPDPLTRDEFIRLIDACRHQQTKNLWSLAVYTGMRHGELVSLAWEDID
                     MKAGTITIRRNYTKLGEFTLPKTEASTNRVVHLIQPAISVLKNQAEMTRLGKQHHIKV
                     QLREYGRSVNHECTFVFNPQVVRKSKQVGFIYKVDSIGDSWETAIKRAGIRHRKAYQS
                     RHTYACWSLSAGANPSFIASQMGHASAQMVFNVYGAWMTDSNAEQIAMLNQKLADYVP
                     WSPK"
     gene            1032..1802
                     /locus_tag="JNZ73_003249"
     CDS             1032..1802
                     /locus_tag="JNZ73_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAY8733429.1"
                     /translation="MKISGLAFNTRHVNCVEGNTTLYALPKPEVVLRWREQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHASRPHSEAIR
                     DAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLH
                     TPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            1855..2250
                     /locus_tag="JNZ73_003250"
     CDS             1855..2250
                     /locus_tag="JNZ73_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733430.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2291..3034
                     /gene="cmoA"
                     /locus_tag="JNZ73_003251"
     CDS             2291..3034
                     /gene="cmoA"
                     /locus_tag="JNZ73_003251"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HAY8733431.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     NAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3031..4002
                     /gene="cmoB"
                     /locus_tag="JNZ73_003252"
     CDS             3031..4002
                     /gene="cmoB"
                     /locus_tag="JNZ73_003252"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HAY8733432.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4167..6596)
                     /locus_tag="JNZ73_003253"
     CDS             complement(4167..6596)
                     /locus_tag="JNZ73_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY8733433.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTILMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPMRVGGVLPEMSAAIAGQASEAADDGGMAAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6621..7721)
                     /locus_tag="JNZ73_003254"
     CDS             complement(6621..7721)
                     /locus_tag="JNZ73_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HAY8733434.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(8109..8855)
                     /gene="cutC"
                     /locus_tag="JNZ73_003255"
     CDS             complement(8109..8855)
                     /gene="cutC"
                     /locus_tag="JNZ73_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAY8733435.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGGLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(8869..9435)
                     /locus_tag="JNZ73_003256"
     CDS             complement(8869..9435)
                     /locus_tag="JNZ73_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY8733436.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            9651..11384
                     /gene="argS"
                     /locus_tag="JNZ73_003257"
     CDS             9651..11384
                     /gene="argS"
                     /locus_tag="JNZ73_003257"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAY8733437.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            11561..>11847
                     /locus_tag="JNZ73_003258"
     CDS             11561..>11847
                     /locus_tag="JNZ73_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HAY8733438.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDTQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLK"
BASE COUNT         2939 a         3005 c         2866 g         3037 t
ORIGIN      
        1 aggaacttga agaaaagtgg ctggatctga aacgaatgga aatctctgca aatgcattca
       61 atcgctatga atccgttgca agaacgatgg ttccgaaaat tggaggcggc agactggtgt
      121 catcggtaac caaagaggaa ttgctgtata tcaggaaaga tttgctgacc gggtatcaga
      181 attcaacgaa aaacaaagca gcagcaaaag gacggagcgt cgttactgta aattattaca
      241 tgacgacaat cgctggaatg tttcagtttg ctgcagatca cggttactta gaagcaaatc
      301 ccttccaggg aattaagcct cttaaaagag ccagggcaga gccagatccg ttaactcgtg
      361 acgaatttat tcgcctgata gatgcttgcc gacatcagca gacgaaaaac ctgtggtcat
      421 tggctgtgta cacaggaatg cgtcacggtg aactggtctc cctggcctgg gaagatatcg
      481 atatgaaagc aggaactatt actatcaggc gcaattatac gaaactcggt gagttcactc
      541 tacctaaaac tgaagcaagt acaaacaggg ttgtgcatct tatccagccc gctatcagtg
      601 tcctgaaaaa tcaggctgaa atgacaagac tgggtaagca gcaccacatc aaggttcaac
      661 tacgtgaata tggacgttca gtgaatcatg aatgtacttt cgtatttaac ccccaggtgg
      721 ttagaaaaag caaacaggta ggttttatct acaaggtaga ttccattggc gactcatggg
      781 aaacagccat taagcgtgcg ggcatcaggc accggaaagc ataccagtca cgacacactt
      841 atgcgtgctg gtcattatct gccggagcaa acccaagctt cattgccagc cagatgggcc
      901 atgcaagtgc ccagatggtg ttcaatgtat acggagcatg gatgactgac agtaatgcag
      961 aacagatcgc aatgctgaat cagaagctgg cagattatgt cccatggtca ccaaagtgac
     1021 acaagagact tattaaaatc agtgggttag ccttcaatac ccgtcatgtt aactgcgtgg
     1081 agggcaacac cacgctttac gccctgccga aacccgaggt tgtcctgcgc tggcgtgaac
     1141 agaccacaga tgacttccgc ttctgtttta agtttccggc gaccatttcg catcaggcag
     1201 cattacgaca ttgcgatgat ttagtgactg aatttttgac ccgcatgtca ccgttggctc
     1261 cgcgcattgg gcaatactgg ctgcaactgc ctgccacatt cggcccacgg gagctgcctg
     1321 cgctttggca ttttctcgat tctctccctg gcgaatttaa ttatggtgtg gaagtccgcc
     1381 atccacagtt tttcgccaaa ggggaagagg aacaaacgct taatcgcggt ttacatcagc
     1441 gcggcgttaa tcgggtgatt ttagacagcc gcccggttca tgcatcacgt ccacacagtg
     1501 aagctattcg cgacgctcaa cgaaaaaaac ctaaagttcc ggtacatgct gtactgacgg
     1561 cgacaaatcc gctgatccgt tttatcggta gtgatgatat gacgcaaaac cgggaattat
     1621 ttcaggtctg gttacaaaaa ttagcgcagt ggcatcagac cactacgcct tatctttttt
     1681 tacatacgcc agatattgcc caggccccgg aactggtaca taccctgtgg gaagacttac
     1741 gtaaaacgct tccagagatc ggagcagttc cggctattcc acagcaatct tctcttttct
     1801 gaatttgcca cctatcatag acaggtgcca tcggccattt taaagggagt ttgtatggta
     1861 agcgcgctat atgccgtttt aagtgcgttg ttattaatga agttctcttt tgatgtcgtt
     1921 cgcctgcgaa tgcagtaccg cgttgcctat ggtgacggtg gttttagcga actgcaaagc
     1981 gctattcgca ttcatggtaa cgcggtggaa tatattccca tcgcgattgt gctgatgctg
     2041 tttatggaaa tgaatggcgc agaaacctgg atggtgcata tttgcggcat cgttttgctt
     2101 gctggtcgtc tgatgcatta ttacggtttt catcaccgtc tgtttcgctg gcgacgttcc
     2161 ggcatgagcg ccacctggtg tgcgctgttg ctgatggtgc tggcgaatct ttggtatatg
     2221 ccctgggagt tggttttctc cctgcgttag cgcacaatac gccactttct ttttcccgga
     2281 tttttacgtt atgtctcacc gcgacacgct attttctgcc cctatcgcca gactgggcga
     2341 ctggaccttt gatgaacggg tagctgaagt cttcccggat atgatccagc gttccgttcc
     2401 cggctattcc aatattattt ccatgattgg tatgttagcc gagcgcttcg ttcaacctgg
     2461 tacgcaggtt tacgatctgg gttgttctct gggcgcggcg acgctctcgg tgcgtcgcaa
     2521 cattcatcat gataattgca aaattattgc catcgacaac tccccggcga tgattgaacg
     2581 ctgccgtcgt catattaacg cctataaagc ccctacgcca gtagacgtca ttgaaggtga
     2641 tattcgcgat atcgccattg aaaacgcatc gatggtggtg ctgaatttta ccctgcaatt
     2701 cctggaacct tccgagcgcc aggcattact ggataaaatt tatcaaggac tgaacccggg
     2761 cggcgcgctg gtgctttcgg aaaaattcag tttcgaagat gccaaagttg gtgaactgtt
     2821 gttcaacatg caccacgact ttaaacgtgc caacggttac agcgaactgg agatcagcca
     2881 gaagcgcagc atgctggaaa acgtgatgct gaccgattcc gtggaaaccc ataaagcacg
     2941 cctgcataaa gccggttttg agcatagcga gctgtggttc cagtgcttta actttggttc
     3001 actggtggca ttaaaggcag aggacgctgc atgatcgact ttggtaactt ttattctctg
     3061 attgccaaaa atcatctttc acactggctc gaaacgctgc ccgcgcagat tgctaactgg
     3121 cagcgcgagc agcagcacgg gctgtttaag cagtggtcca acacggtgga atttctgcct
     3181 gaaattaaac cgtatcgtct ggatttattg catagcgtaa ccgccgaaag cgaagagcca
     3241 ctgagcaccg ggcaaattaa acgcattgaa acgcttatgc ggaacctgat gccatggcgc
     3301 aaagggccgt tctcactgta tggcgtcaac atcgataccg aatggcgttc cgactggaaa
     3361 tgggatcgtg ttctgcccca tctttctgat ttaaccggac gcaccattct ggatgtcggc
     3421 tgcggcagcg gttatcacat gtggcgcatg attggcgcag gcgcgcacct cgcggtgggg
     3481 atcgacccta cgcaactgtt tctgtgccag tttgaagcgg tgcgtaaact actgggtaac
     3541 gatcagcgcg cacatttgtt accgttaggt attgaacagc ttccggcact gaaagccttt
     3601 gataccgtct tttcgatggg cgtgctttac catcgccgtt cgccgctgga gcatctctgg
     3661 cagttgaaag atcaactggt gaatgagggt gaactggtgc tggaaacgct ggttattgat
     3721 ggcgatgaga acacggtgct ggtaccaggc gatcgttacg cgcaaatgcg taatgtctat
     3781 ttcatccctt ccgcgctggc gctgaaaaac tggctgaaga agtgtggttt tgtagatatc
     3841 cgcattgtgg atgtgtgcgt taccactact gaagagcagc gacgcactga atggatggtt
     3901 accgaatctc tgtccgattt cctcgaccca catgatccga gtaagacagt tgaaggttat
     3961 cctgcgccta aacgcgcggt gctgattgcg cgcaagccgt aaaggtctgg tattactgcc
     4021 ggatgcggcg tgaacgcctt atccggccta caaacccttg ctaattcaat ctattgcagg
     4081 gactatgtag gcctgataag catagcgcat caggcagttt tacgtttgca taacctcagc
     4141 gcccgtttcc gggcgctatt cacttcttac gcctgaacag caggcttatc aaatgccgtt
     4201 aacttcggtg cgttacccgt atatttttcg atatacacca gcgcagagtt accggcacag
     4261 gcatttgcca gctgcgagct ggggatatcc gccgttaaca cgttcgcact gccgttttta
     4321 cacaagccgt tttccagatc tggccatgca ccttcatgca cgcataccac gccttttttg
     4381 atcccgtcag tcaccaccgc gcctgtcaaa atctgaccac gtttgttcca gacacgcacc
     4441 agatcgccat tcgcaatacc aaatcgagca gcatcttcgg tgtgaatagt gatcggttca
     4501 cgatcggcaa ccgcatattt tttacgcagt tccgcatagt taagctgact gtgtaaacgg
     4561 tgtgccggat gtgcggtcag aagctgcaac tgcttctcgt cggcggtacc cttccactca
     4621 tcaggcgcaa gccaggttgg gtgtgccggg caatccttat agccaaattt ttccagcgtt
     4681 ttggagtaaa tctcaatttt gccgcttggc gtacccagcg catttttcac cggatcggcg
     4741 cggaaatcac cataacgaac gtactgttcg ttcttctcgc tgcggcgcat ttcgatcagt
     4801 ttattttgct gccagaaggc attaaacatt ggcatagtga cgcgttgcgc acgggcccct
     4861 ttctgagcgg catcatagaa aaatttcagc cacgccattt catctttacc ttcggtatag
     4921 atctcttttc cgccaggttt gagtaattcc gcaagatcgg caaacacgtc aaaatcgtta
     4981 cgcgcttcaa attgcggagc aacagcctgc ttcatcggca caatatgctg gttgctgtaa
     5041 tcaccggtca tcgtcaagtc attgcgctca aacgatgtgg tgatcggtaa tacgatatct
     5101 gcgtgtttgg ctgctgcggt ccagtagcat tcagaaacga cgatcatctc cggtttctgc
     5161 caggctttga tcagacgatt agtatcctgg tgatgggtaa agttaccgcc acccgcccac
     5221 cagatcattt tgatattcgg atacgtttgt tctttaccgt tatgctgata tttgccgcca
     5281 ggattttcca gcgcatcgac gatacgggca acaggaatcg ccgccattcc gccatcatca
     5341 gcggcctcgc tggcctgtcc ggcaatcgcc gcagacattt ccggcagcac gccgccaaca
     5401 cgcatcgggt tgccaccatt ggagtaatga taagagaaac caaaaccgcc gcccggtgta
     5461 ccaatctgcc ctaacatcgc cgccagcgtc accagcatcc agtgtttctg ttcaccatat
     5521 tgctggcgct gaattcccca gcctgccatc agaattgtgc gattcgccgc catcagttca
     5581 gcgagtttga ctatctgcgc ttccggtacg ccagtaattt ccgccgccca gaccgcgctc
     5641 ttcggcgtat tatcgctctt acctgtcaga tactcttcaa actgcggata cccggtagtg
     5701 tatttttcaa ggaacacttt atcgtgtttg ccttgtgtca tcagggtatg cgcaatccct
     5761 aacatcagtg ccacgtcggt gcccatattc ggcgcgatcc aggtggcatt atcgccaaag
     5821 aattcgatgg tttcggagcg gataggatca atggcaatca ctggtttgcc agattttttc
     5881 agctgatgga agtattccag cccttgctca tcggtactgc tccaggcaat ttttaaggta
     5941 ttcagcgggt tcattcccca cagcaccaca acctggctgt tttccagaat cagcggccag
     6001 gaggcctgct gttcatacac ctctacagaa ccgaccacat gcggcatgat cacctgtgct
     6061 gccccggttg aataatcgcc gctatgcccg gaataaccgc ccgccaggtt catataacgt
     6121 tgcagtaagg tttgcgcttt atgcaacacg ccagaagagc gccagccgta agatccggca
     6181 aaaatggccg atggtccgtt agctttacga atacgctcat gttgctcatg aatcagtttt
     6241 aatgcctgtt cccagctcac ctgtacatag gtatcttcgc cacgaccttt cgccggttgc
     6301 agtggattgt cgagatagct ttttctcacc atcggatgct gaatacgcgc cgtggtgtgt
     6361 acctgatccg ccgccgtaga ctgtaaggag ttcggtatgg ttttcgccag cgcgcctgtc
     6421 gaagaaacaa tcttgccgtc cttcacttct acgctcatcg ctccccaacg tcccgcggtg
     6481 aggattttac cgcccttctc ttctgcccat gcggggagcg gtgctgccga tgtcaccacc
     6541 agcgctccag cggcaatacc gctgtgttta ataaattcac gtcttgttaa tgccataact
     6601 tcctccctga tcaacgagga tcactgtttc tcggtaatat ctttggcgtt gtactggaaa
     6661 taccgcgtta aaatgtccag ttcgttttcg ctcatgctgg ttcgtgcccc cattcctttg
     6721 gcaatggacg gccacgcatt gacggtgtaa tggtcggcgg caatagaggc atgacaacca
     6781 gcgcaatagg tatcgtcaag tttttcagcg tattgccata gcggtttacg gtccgctaat
     6841 gcgggatcgg taagcgcacc ctgtaaagac gcctgacgcc attgattgcc gtattcgtca
     6901 gcctgccatt ccccgtttac cgtgagcgcc ttgatacctt cttcacttaa tgtggctagc
     6961 gccagccgtt gacctgccgc caggtagagc gtgttttcac tgccctgcat ttgataaccc
     7021 tgcaacagaa cgatcggctg tttgccactg gcatcaacga cggtgagatc ggtaccagga
     7081 ttcacggtag ccagctcgcc tatgcgagaa gttttgaaag gataaatatg tgcgccatta
     7141 gtcactgaag tagcggcctg actttccagc tcatgcgccg cgttgtcatc catttttatt
     7201 tctggcggaa aatgggcaat gcctttatga caatcgatac aggtttcgcc gcccttttgt
     7261 gctttgttat gcattttctg cgcagattca ctttgcgagg caatatccat ggcatcaaaa
     7321 gaatggcaac tacggcacgt tgcagagtca gttgctttta attctttcca tactgtttcg
     7381 gccatttcct ggcgatgagt ttcgaactta tcgtcactgt ctattttgcc gctaacaaat
     7441 tcatgataaa tatctttaga tgcttttaat ttagcaaata aataatccat ccctgacttt
     7501 ggaatatggc aatcggcaca ttccgcacgt atccctttct ggttcgaaaa gtggacagtt
     7561 ccctgatatt cctcaaaagg tttactcatc gagtggcagg aaaggcaaaa tgctgtatcc
     7621 gacgttttat gtaaggcttt ttgcgccagc aataacccgc cgccaccaac cacaaccgct
     7681 atcagcaaaa acaataaccc aatgcgtttt ttccctcgca tagattcctc taaataaatt
     7741 ccagaacacc gaaatcattg tataattaaa tacacaacga attaaccgct tgcataactc
     7801 ggcacaacac tgcctgaaat aatcgataaa gaatatgatt tattacaatg taatcattaa
     7861 ttgctaagga ataacccagt tgctatttag aatatatatt tatcagtttt agcaatttaa
     7921 atcccataat caatgtgaat ataaaaaata tttttagcac aacgctatgc gcactgtcac
     7981 ccgctcaaca gatcacggtt atcgttcgtt ttttatactg ctcagggata aaaaaaggcc
     8041 cctgttgaaa ttgcaggggc ctggtacgag caagcatcat attgggcgac atgatgcaac
     8101 ggtaaaaatc atttggcctg atggcgttca atgattcctt tcatttcagc aaccgccgcc
     8161 ccgtctacga tataacgcga atactcgtcc gcgtgttcat ctgatgacat ggacaatcct
     8221 tgattacgat aacgcatcgg tgaggcttgc cacgctcccg cggagctatg gacttccagc
     8281 actccggcat cgaggaagtg gtgcaggttt tctgcacgga ctcctgctcc agccataatg
     8341 attggagcat cacgatgggc aataagttcc ataatttttg ataaaccttg cagtgcgtct
     8401 gatttttgcc ctgatgtcag tacccgggca atgcccaatt ccgcaagatt attgagtgta
     8461 tataaagggt tagcgcacat atcgaaggcg cgatgaaaag tcactgccag cggaccggca
     8521 gcagccatta ttttttccat tcgtggcata tcgacattcc catcaacatc gagaacgccc
     8581 gtcaccagtc caggaaaacc taattcgcgg accgtgcgca catcctcaag aatggcggca
     8641 aactcaccgt cgctgtagca aaaatcacca ccgcgtgggc gaattatcgg atgcacaggg
     8701 atcgtcaccc gctggcgcac ggatttcagt ccccccaacg acggcgttaa gcccccctct
     8761 tttggggctg cgcataattc aactctgtct gcgccgtttt gctgcgccgt tagtgcacat
     8821 tccatgctgt aacagcaaat ttccagtaat gccatttttg ctccttaatt acgctgactg
     8881 ctcgctggca acgatctctt caatggacca cggatgaaac ttaatagttg ttttgccatc
     8941 ggtgaccgcc agcgtcggat tcggtaatcg ctcatgctca ccttttggtg aactggtttt
     9001 aactgagatc cccggtaggc tgagtccttc atcagaaagc aatgccagcg cacgggcatt
     9061 cagcgtttct ggatcacccg gcagaacgat ttcaatatgt tcccaacctt cgtgtgggta
     9121 acgtttttcc ccaggccacg gtagctccac attggaaaac tgccagtgcg caacctgtac
     9181 cggttcatgc aatttaaaca gacaaatcgg tctgccattg atcatttttt ctgacaaaag
     9241 ctcgccacac tgttcaaaac cgcgacgcca gcgctcagcg gtgacgtttt gatggcaacg
     9301 caaagaaatg tgatcggcag tcagcggagt gatatccaac cccagacggc gggaaagttc
     9361 atctaatgcg tgggtaaatc gcggtaaatc cgatgcaata tcctgcagct cgtcgataga
     9421 ttgccagttc gccataatca ctcttcgtct ttcagtaaaa gcgttaattt accctgttgc
     9481 cctgtgccaa ccaaccgctg atttcacgcc gcttctgatg caatagtgaa aacggcaata
     9541 cgccacgcgc acgttgctga cgaaaacagc catttgcagt atactcccgc cctaatttct
     9601 ttaactggtg cgggcaattt ttgctcgctt catcaatgta aggtattccg gtgaatattc
     9661 aggctcttct ctcagaaaaa gtccgtcagg ccatgattgc ggcaggcgcg cctgcggatt
     9721 gcgaaccgca gattcgtcag tcagcaaaag ttcagttcgg cgactatcag gctaacggca
     9781 tgatggcagt tgctaaaaaa ctgggtatgg caccgcgaca attagcagag caggtgctga
     9841 ctcatctgga tcttaacggt atcgccagca aagttgagat cgccggtcca ggctttatca
     9901 acattttcct tgatccggca ttcctggctg aacatgttca gcaggcgctg gcgtccgatc
     9961 gtctcggtgt tgctacgcca gaaaaacaga ccattgtggt tgactactct gcgccaaacg
    10021 tggcgaaaga gatgcacgtc ggtcacctgc gctctaccat tattggtgac gcagcagtgc
    10081 gtactctgga gttcctcggt cacaaagtga ttcgcgcaaa ccacgtcggc gactggggca
    10141 ctcagttcgg tatgctgatt gcatggctgg aaaagcagca gcaggaaaac gccggtgaaa
    10201 tggagctggc tgaccttgaa ggtttctacc gcgatgcgaa aaagcattac gacgaagatg
    10261 aagagtttgc cgagcgtgca cgtaactacg tggtaaaact gcaaagcggt gacgaatatt
    10321 tccgcgagat gtggcgcaaa ctggtcgaca tcaccatgac gcagaaccaa atcacttacg
    10381 atcgtctcaa cgtgacgctg acccgtgatg acgtgatggg cgaaagcctc tacaacccaa
    10441 tgctgccagg aattgtggcg gatctcaaag ccaaaggtct ggcagtagaa agcgaagggg
    10501 cgaccgtcgt attccttgat gagtttaaaa acaaggaagg cgaaccgatg ggcgtgatca
    10561 ttcagaagaa agatggcggc tatctctaca ccaccactga tatcgcctgt gcgaaatatc
    10621 gttatgaaac actgcatgcc gatcgcgtgc tgtattacat cgactcccgt cagcatcaac
    10681 acctgatgca ggcatgggcg atcgtccgta aagcaggcta tgtaccggaa tccgtaccgc
    10741 tggaacacca catgttcggc atgatgctgg gtaaagacgg caaaccgttc aaaacccgcg
    10801 cgggtggtac agtgaaactg gcagacctgc tggatgaagc cctggagcgt gcacgtcgtc
    10861 tggtggcaga aaagaacccg gatatgccag ctgacgagct ggaaaaactg gctaacgcgg
    10921 ttggtattgg tgcggtgaaa tatgcggatc tctccaaaaa ccgcaccacg gactacatct
    10981 tcgactggga caacatgctg gcgtttgagg gtaataccgc gccatacatg cagtatgctt
    11041 acacgcgtgt attgtccgtg ttccgtaaag cagaaattaa cgaagagcaa ctggctgcag
    11101 ctccggtaat catccgtgaa gatcgtgaag cgcaactggc agctcgcctg ctgcagtttg
    11161 aagaaaccct caccgtggtt gcccgtgaag gcacgccgca tgtaatgtgt gcttacctgt
    11221 acgatctggc cggtctgttc tctggcttct acgagcactg cccgatcctc agcgcagaaa
    11281 acgaagaagt gcgtaacagc cgtctgaaac tggcacaact gacagcgaag acgctgaagc
    11341 tgggtctgga tacgctgggt attgagactg tagagcgtat gtaatcgatt tttcgtgaga
    11401 gtgaagcctg atcagggtta gccgatcagg cttttttatt gccatctaaa tgtattctga
    11461 aaacggacat gccattgttt tctcactgtt gagtaagagg ccagaagcgt aatatccagg
    11521 cccaggaaaa cgataactgt tcaatttaag gaataccgaa gtgtttaaat ttcttgtatt
    11581 aacattaggc attatctctt gccaggctta cgcagaagat acggttatag taaacgacca
    11641 tgacacttca gccatcaaag attgttggca aaaaaattca gatgatgata ctgacattaa
    11701 cgtgatcaaa tcatgcctgc gacaagaata caatctcgtc gatacgcaat taaataaagc
    11761 ctatggtgaa gcttatcgtt atatagaaca agtgccacgc acaggtgcaa aaaaacttga
    11821 tactgaacaa cttaacttgc ttaaaaa
//
LOCUS       DADMGC010000120        11785 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.120, whole genome shotgun sequence.
ACCESSION   DADMGC010000120 DADMGC010000000
VERSION     DADMGC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.120"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            202..537
                     /locus_tag="JNZ73_003259"
     CDS             202..537
                     /locus_tag="JNZ73_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HAY8733439.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            630..713
                     /locus_tag="JNZ73_003260"
     CDS             630..713
                     /locus_tag="JNZ73_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733440.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            813..1634
                     /locus_tag="JNZ73_003261"
     CDS             813..1634
                     /locus_tag="JNZ73_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAY8733441.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1673..2002)
                     /gene="mdtI"
                     /locus_tag="JNZ73_003262"
     CDS             complement(1673..2002)
                     /gene="mdtI"
                     /locus_tag="JNZ73_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HAY8733442.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1989..2354)
                     /gene="mdtJ"
                     /locus_tag="JNZ73_003263"
     CDS             complement(1989..2354)
                     /gene="mdtJ"
                     /locus_tag="JNZ73_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HAY8733443.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2765..3799
                     /gene="tqsA"
                     /locus_tag="JNZ73_003264"
     CDS             2765..3799
                     /gene="tqsA"
                     /locus_tag="JNZ73_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAY8733444.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVHFAFVWGLLAFALNYIPNIGSVLAAIPPIAQLLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3824..5212)
                     /gene="pntB"
                     /locus_tag="JNZ73_003265"
     CDS             complement(3824..5212)
                     /gene="pntB"
                     /locus_tag="JNZ73_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HAY8733445.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARSINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5223..6755)
                     /gene="pntA"
                     /locus_tag="JNZ73_003266"
     CDS             complement(5223..6755)
                     /gene="pntA"
                     /locus_tag="JNZ73_003266"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HAY8733446.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFNE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7279..8223
                     /locus_tag="JNZ73_003267"
     CDS             7279..8223
                     /locus_tag="JNZ73_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY8733447.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            8409..9791
                     /locus_tag="JNZ73_003268"
     CDS             8409..9791
                     /locus_tag="JNZ73_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8733448.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQDAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9828..10550
                     /gene="folM"
                     /locus_tag="JNZ73_003269"
     CDS             9828..10550
                     /gene="folM"
                     /locus_tag="JNZ73_003269"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HAY8733449.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10547..10882)
                     /locus_tag="JNZ73_003270"
     CDS             complement(10547..10882)
                     /locus_tag="JNZ73_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HAY8733450.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPIFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            11011..11754
                     /gene="rstA"
                     /locus_tag="JNZ73_003271"
     CDS             11011..11754
                     /gene="rstA"
                     /locus_tag="JNZ73_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HAY8733451.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
BASE COUNT         2994 a         3013 c         2833 g         2945 t
ORIGIN      
        1 gctttgtaag tagtataata gttaacccga tcaagactac tattattggt agctaaattt
       61 tccttaagtc acaatacgtt attatcaacg ctgtaattta ttcagcgttt gtacatatcg
      121 ttacacgctg aaaccaacca ctcacggaag tctgccattc ccagggatat agttatttca
      181 acggccccgc agtggggtta aatgaaaaaa caaattgagg gtatgacaat gaaaaaagta
      241 ttagctctgg ttgttgccgc tgctatgggt ctgtcttctg ccgcctttgc tgcagagact
      301 gcgaccacac ctgctccgac tgcgacgacc accaaagcag cgccagcgaa aactacacat
      361 cataaaaaac agcataaagc agcacctgcc cagaaagcgc aggcggctaa aaagcatcat
      421 aaaaatacga aagctgaaca gaaagcccct gaacaaaaag cgcaggcagc gaagaaacac
      481 gccgggaaac acggccatca gcaaccggca aaacctgctg cacaacccgc agcgtaagtt
      541 ttcaacagta atgctggcgc gccccctcgc gcctgaaaat tacggtgcta agcgggtaac
      601 gtttagcacc gcctttagcg gagggtagta tggtgggccg ttatcgcttt gagttcattc
      661 ttatcatcct tattttatgc gcactgattg ccgcccgttt ttatctttcc tgactgtagt
      721 tatctgattt tactcccact ttcatcccgt cccgtctata gtatttatga gggtttgctt
      781 ttaataatca taattaccca ccagagtgtg atatgcgtac aaccattgct gtagtgttgg
      841 gtgcaattag tttgacgtct gcttttgtgt ttgcagataa accagacgtt gccaaatcgg
      901 caaacgatga ggtcagcacc ctgttttttg gtcatgatga tcgtgtgcca gtgaatgaca
      961 cgacccaatc accgtgggat gcggttgggc aactggaaac ggccagcggc aatttatgta
     1021 cggcgacgct gattgcaccc aatctggcat taacggcagg acactgttta ttgacgccgc
     1081 ctaagggtaa agcggataag gcggtagcgc tgcgttttgt gtcaaataaa ggtctttggc
     1141 gctatgagat ccacgacata gaaggccgcg ttgatccgac actgggaaag cggttaaaag
     1201 cagatgggga tggttggatt gttcctcccg cagctgcacc gtgggacttc ggattgattg
     1261 tgctacgtaa tcccccttct ggcattacac cgttgccgtt atttgaggga gataaagccg
     1321 cgcttactgc cgcattaaaa tcggctggtc gtaaagtgac tcaggcggga taccctgaag
     1381 atcatctcga tacgttatac agtcatcaaa actgtgaagt gactggctgg gcgcaaacgt
     1441 cggtgatgtc acatcagtgc gataccttgc cgggtgacag cggttcgcct ctgatgttgc
     1501 ataccgatga cggctggcaa ttaattgggg tgcaaagttc ggctcctgcc gcgaaagatc
     1561 gctggcgcgc cgataaccgg gccatttctg ttaccggttt tcgcgacaag ctggatcaac
     1621 tgtcgcaaaa ataatgttca aacgctgccc gacagcgcgg gcagcgtctt catcaggcaa
     1681 gtttcaccat gatcattcca gccaacagca agaccaggcc aatccagcct ttacgattta
     1741 accgttgacc aaacaagatc caacctgcgg ctaacgtggc ggcaatacca aacccgcccc
     1801 acaatgcata agcgacagac aagtcgatcc ctttaacggc ctgagaaagc gcactaaagg
     1861 ccgccagcac cgccgccagg gagagcaagc caaatatttt gcgacgaaag ccgtcagaaa
     1921 atttcaaaaa gacgttagca acgatttcca gcacgattgc caatgccagc caggcggcgt
     1981 gaacccattc aaactgcgcc atggttcacc tccagttcag gtttacgcgc tttacgggta
     2041 cctgatttga tcaacactat cccggcgacc agggtggtta acccggcaat tttcatcagc
     2101 gataaacttt cgtcgaataa caaaacgcta aacaaggtaa taaataaaat accgatacct
     2161 tcccacagcg cataagctac gcctaaggcg atttttttaa cggcgaaaga gagaaatata
     2221 tacgacagag aaatcatcac cagcattaaa ataaagccgc catttccctc actgacgctc
     2281 gcccatttca ttgacagcgt accggtaatt tctgtagcaa tagccagacc taataaaatc
     2341 caataaatat acattgtcct tctcctgcaa gagaactatt ttaattttcg cttaattcag
     2401 cgaaaccaaa gtaaagtggt caagccctgc gcatagggca agcttaggca gaagaaagga
     2461 ctaaagcgcg ttgcgccagt gctgctcacc tacgagcaag atagtggatg aggtacgaag
     2521 atgggtaaat gtagaaaata acgtcctgaa caaattgtcc ataatattac aattatccgc
     2581 agtgttgctt ctcgtcatcg cagatgataa ttgtcctcgg tagttgaaca cgcctgattt
     2641 gtatcatagc ttaagaatta actcaaaata ttttcacttc tttacctgag cggtttgatt
     2701 ttcgttatga tgacggagcg aaaaagacat tattattagc aaaggaagaa aaacggggac
     2761 aagcatggca aagccgatca tcacgctcaa tggcctaaaa atcgtcatta tgttgggaat
     2821 gctggtcatt attctctgcg gtatccgttt tgccgccgag atcatcgtgc cgtttattct
     2881 cgcattattt attgctgtta ttcttaaccc gctggtgcaa catatggtcc gctggcgtgt
     2941 gccgcgtgta ctggcagtgt cgattttgat gacaatcatc gtgatggcga tggtgttgct
     3001 attagcttat ctgggttccg cgctcaacga gttgacgcgg acgttaccgc aatatcgcaa
     3061 ctctattatg acgccgctgc aagctcttga gccgttgttg caacgcgtag ggattgacgt
     3121 ctcagttgac caactggcgc attacattga tccgaacgcg gcgatgacgt tgctcaccaa
     3181 cttattgacg cagttatcta atgccatgtc atcaatattt ttattgctgc tgacggtgct
     3241 gtttatgctg ctcgaagtgc cacaattgcc cggaaaattt cagcaaatga tggcgcgtcc
     3301 ggttgaaggg atggcggcga ttcaacgtgc gattgacagt gtttctcatt atctggtgct
     3361 gaaaacagcc atcagcatca tcaccggctt ggtcgcctgg gcgatgctcg ccgcactcga
     3421 tgttcacttc gcttttgtct ggggattgct ggcctttgcg cttaattaca tccctaatat
     3481 tggttcagtt ctcgcggcaa tcccccctat cgctcagcta ctggtgttta atggcttcta
     3541 cgaagcgttg ctggtgctgg cgggatatct gctgattaat ctggtcttcg gcaatattct
     3601 ggagccgcgc atcatgggac gtgggctggg gctttcgaca ttggtggtat ttttgtcgtt
     3661 gattttttgg ggatggttgt taggaccggt gggtatgctg ctttccgtgc cgttgacaat
     3721 tattgtcaaa attgcgcttg aacaaacagc gggaggtcaa agcatcgccg ttctgttaag
     3781 cgatctcaat aaagagtgac ggcctccgca gaggccgtca gggttacaga gctttcagga
     3841 ttgcatccac gctggctttg gcgtcaccaa acagcatgtg ggtgttttcc ttgaagaaca
     3901 gcgggttttg cacaccagca tagccagtgt tcatcgaacg tttaaagaca atcacgttct
     3961 gcgctttcca cacttccagc acaggcatac cagcaatcgg actcttcgga tcatcctgcg
     4021 ccgccgggtt aaccgtatcg ttagcaccaa tcaccagtac ggtatcggta tcagcaaagt
     4081 catcgttgat ctcgtccatt tccagcacga tgtcatacgg tacttttgct tcagccagca
     4141 atacgttcat atgtccaggc aaacgccccg caaccgggtg gataccgaaa cgcacattaa
     4201 tactacgagc gcggagtttc tcggtaattt cagcgacagg atattgcgcc tgcgcgactg
     4261 ccatgccgta ccccggagta atgatcactg aatgggagtt tttcagcagt tccgctgtct
     4321 cttctgcggt gatttcgcgg tgctcaccca cttcctgatc atcgccagta gaagagccgt
     4381 cggtgccgaa accacccgca ataacgctga taaaggaacg gttcatcgcc ttacacataa
     4441 tgtaagaaag gatagccccc gaagaaccga ccagcgcacc ggtcacaatc agcaggtcgt
     4501 tgctgagcat aaagcccgca gccgcagccg cccagccgga gtacgagttc agcatcgaca
     4561 ccaccaccgg catatctgca ccaccgatgg aggcgactaa atgccagccg aataccagcg
     4621 caatggcggt cattatcagc aatgccagca cttgcaggcc gacgctgtcc gtgcgaacaa
     4681 atacaatcag cagcaagaag gaaacgacca gagccgccag gttcattttg tgacggtttg
     4741 gcagcatcaa cggtttagac gaaatcttgc cacacagttt gccgaacgcc accaccgagc
     4801 ccgtgaacgt taccgccccg atgaagatac cgaggaacac ttccgtcagg tgaatattga
     4861 ccagaatcgg tgccattccc gcgtcatgat gcagataact gttaaagcca accagcactg
     4921 ccgccagacc cacgaagctg tgcaggatcg ccaccagttc tggcatttcg gtcatttcaa
     4981 ctttcttcgc cagccggata ccaattgccc caccaatgac catcgccagc aagatccagc
     5041 caacattacc cgtatccggt ccaaaaatgg ttgcgattaa cgcaatcgcc atcccggcga
     5101 taccgaagtt gttaccctgg cgagacgttt catgtttcga aagaccggcc agactgaaga
     5161 taaacaggat cgcggcaaca atgtatgcag ctgtaactaa tcctccagac atatgttacc
     5221 ccttaatttt tgcggaacat tttcagcatg cgctgagtca cggtgaagcc accgaaaata
     5281 ttaatgctgg ctataagcac cgcgataaaa ctaaggaagc taacccagcc tccctggcca
     5341 atctgcaaca gtgctccgac aacaataatc cctgaaatcg cgttggtaac cgacatcaac
     5401 ggtgtatgca gcgcgtgcga tacattccac accacgtaat aaccgacaac gcaggccagc
     5461 gcgaaaacgg tgaagtgccc aagaaactct ttcggcgcaa cgcttgacag ccaaccgaac
     5521 aggatgattg ccagcgccat caacgcgtat ttacgccacg gtgagcaggc acatttttcc
     5581 tcagttttca cttccggtgc cgctttttgt gccgcctgcg gctgagctga tacctgaatc
     5641 ggcggtgccg gccaggtaat ttcgcccgca cggatcacgg tcacgccgcg aatcaccaca
     5701 tcatcaaaat caacagtgat attgccgtct ttctctttgc acaacagttt cagcagatta
     5761 acgaggttag taccgtaaag ctgtgaggat tgcgtcggca gacggcccgg aagatcggta
     5821 taaccaatca ctttgacacc attttccgta ctgaagattt cacccggcac gatgtattca
     5881 cagttgccac cgttttgggc tgccaggtcg acaatcacac tgcccgcctt catggagtca
     5941 accatttcac gggtaattag cttcggcgct ggtttgcctg gaataagcgc ggtggtgaca
     6001 atgatatcga cctcttttgc ctgggcggca aagagttcca tttccgcttt gatgaacgcg
     6061 tccgacatca ctttggcata gccatcgccg ctgcccgctt cctcgttaaa atccagctcg
     6121 aggaattccg cgcccatact ttgaacttgt tctttcactt ccgggcgggt gtcgaatgca
     6181 cgcacaatcg cgccgagact gtttgctgcg ccaatggcgg ccaaacctgc aacaccggca
     6241 ccaatcacca tcacttttgc cggtggcact ttcccggccg cagtgatttg cccggtaaag
     6301 aagcgcccaa attcatgtgc cgcttcaaca atggcgcgat aaccggcgat attcgccatc
     6361 gagcttagtg cgtccagcga ttgtgcgcgt gagattcgcg gcacagagtc catcgccatc
     6421 acggtcacgt tacgttccgc aagtttttgc attaattccg gattctgcgc aggccagata
     6481 aaactcacca gcgttgtccc gggattcagt aacgcaattt catcatctaa cggcgcattg
     6541 accttcagaa tgatctctga ctgccagacg ctattccctt ctacaatttc agcgcccgct
     6601 tgcacaaacg ctttatcgtc aaaacttgcc agttgacccg cgccgctctc taccgcgacg
     6661 gtaaaaccca gtttcagcag ctgttccact gtttttggcg ttgctgcaac acgggtttca
     6721 ttggttaacc gttctcttgg tatgccaatt cgcatgatat tcccttccat cggttttatt
     6781 gatgatggtt tgcctgtgtc aggagccaca caagccgctc atgtacgagc taaatgttac
     6841 tccgttaaaa taaattagta acaaacgccc tataacgtac tgaaaattat gcctgtgatc
     6901 tagcgccaaa aatcagtatt tcggcgtgaa ctcgcaaaat attaacgatt cagccgtgat
     6961 agtgggataa acaccttaga acgccggata aagactgata attgtcttcg acggtcgggt
     7021 aaaacgagac atcgccccgg cacgaatcac tacttaacat taaattaact tatacaattc
     7081 agttgcttca gtagtaatga tgctgatacg gctgtttttt aagcatagac ggtcatttga
     7141 gcaggattaa aattggttta aggaatgtga tatgaaaaat gacgcagaca gttacaccgt
     7201 ttaaatgcaa taatcagcca cgtttctcgt taataacaat accagtacct ggtttgcgca
     7261 aggcgaagga ttatttttat gaagcttaag aacaccctcc tggcgtcggc actgctttct
     7321 gctacggcat tctccgttaa cgcagcaaca gaactgacac cggagcaagc ggcagcggtt
     7381 aaaccttttg accgtgtagt ggttaccggt cgttttaatg ctattggcga agcggtgaaa
     7441 gccgtttctc gtcgcgcaga taaagaaggt gccgcctctt tttatgttgt cgacacttct
     7501 gattttggta acagcggtaa ctggcgtgtg gtcgctgacc tctataaagc cgatgctgaa
     7561 aaagcagaag aaacaagtaa tcgcgtaatt aacggtgttg tcgaactgcc gaaagatcag
     7621 gctgttctga ttgaaccgtt tgacacggtc accgtccagg gcttctatcg tagccagcca
     7681 gaagtcaatg atgccatcac caaagcggca aaagcgaaag gtgcctactc tttctacatc
     7741 gttcgtcaaa tcgatgccaa ccagggcggc aaccagcgta ttactgcatt catctataaa
     7801 aaagatgcta agaaacgtat cgtccagagc ccggatgtga tcccggcaga ttccgaagca
     7861 ggacgtgcag ctctggctgc cggtggcgaa gccgcgaaga aagttgagat cccgggtgtt
     7921 gcgactaccg catcaccaag ttctgaagtc ggtcgcttct ttgaaaccca gtcatcaaaa
     7981 ggcgggcgtt acaccgtcac gctcccggat ggcactaaag tcgaagaact gaacaaagcg
     8041 acagcagcga tgatggtccc gttcgacagc atcaaattct ctggcaacta cggcaacatg
     8101 accgaagtct cctatcaggt tgcgaaacgt gccgcgaaga aaggtgctaa gtattaccac
     8161 atcacccgcc agtggcagaa acgtggtaat aacctgaccg tcagcgcaga tctgtataaa
     8221 taactattca gttactccta taaggcggct tgatgccgcc tttcgcattt ttagtcacaa
     8281 atttccccgc gaccattgca tgcagacaca tcttttcgta aaatcccgcg cttcagtggc
     8341 aaactccatt tttatgcgtt ttgccctaat atttattctt cttatcacgt tttaatcact
     8401 ggatatcgat ggaaaagaaa ctgggactga gcgcactcac cgcgctggta ttaagctcaa
     8461 tgctgggcgc gggtgttttc agtctgccgc aaaatatggc ggcagttgcc agcccggcag
     8521 cactgctcat cggctggggt attactggcg ctggcatttt attgctggcc tttgccatgc
     8581 tgatcctcac gcgcattcgt cccgaacttg atggcggtat ctttacctat gcccgcgaag
     8641 ggtttggcga gctaatcggc ttttgttccg catggggata ctggctgtgc gcggtcatcg
     8701 ccaacgtctc ctatctggtg atcgtttttt ccgcgttaag cttttttacg gacacgccgg
     8761 aattgcgcct gtttggcgat ggtaatacct ggcaatcgat agtcggtgca tcggctttat
     8821 tgtggattgt tcactttttg atcctgcgcg gggtgcaaac cgctgccagc attaacctgg
     8881 tggcgacact ggcaaaattg ttgccgctgg gtctgtttgt tgtgctggcg atgatgatgt
     8941 tcaaactgga taccttcaag ctcgacttca ccggacttgc acttggcgta cctgtttggg
     9001 aacaggtgaa aaacaccatg ctgatcaccc tgtgggtttt cattggtgtg gaaggtgccg
     9061 tggttgtttc tgcgcgtgcg cgtaataaac gtgatgttgg caaagcgaca ctgctggcgg
     9121 ttctctccgc tctgggcgtt tacttgctgg taacgctgct ttcgctgggt gtggtggcac
     9181 gtcctgaact ggctgaaatt cgtaacccgt caatggccgg tctgatggtg gaaatgatgg
     9241 gaccatgggg cgaaatcatc atcgctgccg gtttgatcgt ttccgtttgc ggcgcgtact
     9301 taagctggac catcatggcg gcagaagttc cgttcctggc tgccactcat aaagcattcc
     9361 cacgcatttt tgcgcgccag gacgcacaag ctgcgccatc cgcctcactg tggttgacca
     9421 acatctgcgt gcaaatttgt ctggtactca tctggctgac cggttccgat tacaacacgt
     9481 tgctgaccat cgcctcagaa atgattctgg tgccctattt cctggtcggc gcgttcttgc
     9541 tgaaaatcgc aacccgtcca ctgcataaag cggtaggtgt cggtgcctgc atttatggct
     9601 tatggttatt gtacgcctct ggtccaatgc acttgctgtt atccgttgtt ctgtatgcac
     9661 cggggctgct ggttttcctg tatgcgcgca aaacgcatac acatgataat gtactgaacc
     9721 gtcaggaaat ggtgctgatt ggcatgctgt taattgcctc agttccggcg acctggatgc
     9781 tggtgggata acgttcatcc catcgttgga tagcaaagga gataacgatg ggtaaaaccc
     9841 agcccttgcc aatattaatt actggcggag gtcgtcgcat cggcctcgcc ctcgcatggc
     9901 atttcattaa tcaaaagcaa ccggtgattg tcagctatcg gacacactat ccagccattg
     9961 atggactgat taatgcaggt gcgcagtgta ttcaggctga tttttcgacc aacgacggtg
    10021 tgatggcgtt tgccgatgaa gtactaaaaa ccacccatgg tctgcgtgct attttgcata
    10081 acgccagtgc gtggattgcg gaaaaaccgg gtgcgccact ggccgacgta ctggcttgca
    10141 tgatgcagat ccacgttaat accccatacc tgctcaacca tgcgctggaa agattactgc
    10201 gtgggcacgg acacgccgcc agcgatatca ttcactttac cgattatgtg gtggagcgcg
    10261 gcagcgacaa acatgttgcg tatgctgcaa gcaaagcggc actggataat atgacccgct
    10321 cgtttgcccg caagctggca ccggaagtga aagtgaattc tattgcgcca tcgctgatcc
    10381 tgtttaatga acatgatgat gccgaatatc gacaacaggc gctgaataaa tcactgatga
    10441 aaaccgcgcc tggcgagaaa gaagtgatcg acctggtcga ttacttactt accagttgct
    10501 ttgtcaccgg acgcagtttc ccacttgatg gcggtcgtca tctgcgttaa tgcagcttta
    10561 tccagcatat aatcaacacc catgcgctta tcccccaaca cgcaacagaa ccaacaaaag
    10621 cggcgggcaa gcgcatcatt ccggtgaaat accacagcga caccaggtaa acaaaatagg
    10681 gaataatcga ccacatacta aaaatgatgg ttgcgcgtaa ggcttcaatg ccgcgttcgc
    10741 tggcaacaat ataatgcgcg ataagcgcaa agatcgggaa aagtggaatc agcccggcga
    10801 tataataatt tttcgttttt gctaaaacac caatcaacag cactaccagc gcaccgagcg
    10861 cggctttgat taccagcccc atctttttac cttaacactt ccataacaag tcatcagtag
    10921 aatacctgat gaaaacttgt ttagaaacga ttgatagtaa gtaaaaacag cgcggtgtat
    10981 tgtgacgttt ttatatctac cgtgaatgtt atgaacacta tcgtatttgt ggaagatgat
    11041 gcggaagtcg gttcactgat tgccgcgtac ctggcaaaac atgatatgca ggttaccgta
    11101 gaaccgcgcg gcgaccaggc cgaagaaacc attttgcgag aaaatccgga tttggtgtta
    11161 ctcgacatca tgctaccagg caaggacggc atgaccattt gtcgtgattt acgcgcaaag
    11221 tggtctggac cgattgttct tctaacctct ctcgatagcg atatgaacca catcctggca
    11281 ctggaaatgg gtgcctgcga ctatattctc aaaacgacgc cccctgctgt tttgctagcg
    11341 cgtttacgtt tgcatttgcg tcagaatgag caagccacac tgaccaaagg tcttcaggaa
    11401 acgtctctga ctccctacaa agccctgcat ttcggcacgt taactatcga ccccatcaac
    11461 cgcgtagtca ccctggctaa cactgaaatc tcgctctcga cagctgattt cgaattattg
    11521 tgggaactag ctacgcatgc cgggcaaatc atggaccgcg atgcattgct gaaaaattta
    11581 cgcggcgtca gttatgacgg actggatcgt agcgtggacg tggctatttc gcggttaaga
    11641 aaaaaactgc tcgataacgc cgcagaacct tatcgcatta aaactgtgcg taacaaaggc
    11701 tatctttttg cgcctcatgc atgggatagt gacaataaac aatcagatga ctaatatttg
    11761 ttcatctgaa aaagagaaca attaa
//
LOCUS       DADMGC010000121        11737 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.121, whole genome shotgun sequence.
ACCESSION   DADMGC010000121 DADMGC010000000
VERSION     DADMGC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.121"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(34..1398)
                     /gene="tdcG"
                     /locus_tag="JNZ73_003272"
     CDS             complement(34..1398)
                     /gene="tdcG"
                     /locus_tag="JNZ73_003272"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY8733452.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALHRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1470..1859)
                     /locus_tag="JNZ73_003273"
     CDS             complement(1470..1859)
                     /locus_tag="JNZ73_003273"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HAY8733453.1"
                     /translation="MKKIIETQRALGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(1873..3980)
                     /gene="tdcE"
                     /locus_tag="JNZ73_003274"
                     /pseudo
     CDS             complement(1873..3980)
                     /gene="tdcE"
                     /locus_tag="JNZ73_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
     gene            complement(3990..4166)
                     /locus_tag="JNZ73_003275"
     CDS             complement(3990..4166)
                     /locus_tag="JNZ73_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate acetyltransferase"
                     /protein_id="HAY8733454.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTEL"
     gene            complement(4200..5408)
                     /gene="tdcD"
                     /locus_tag="JNZ73_003276"
     CDS             complement(4200..5408)
                     /gene="tdcD"
                     /locus_tag="JNZ73_003276"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HAY8733455.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLALHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5434..6765)
                     /gene="tdcC"
                     /locus_tag="JNZ73_003277"
     CDS             complement(5434..6765)
                     /gene="tdcC"
                     /locus_tag="JNZ73_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HAY8733456.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKVQNIPVLSYLANHFASMAGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(6787..7776)
                     /gene="tdcB"
                     /locus_tag="JNZ73_003278"
     CDS             complement(6787..7776)
                     /gene="tdcB"
                     /locus_tag="JNZ73_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HAY8733457.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAILGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(7875..8813)
                     /gene="tdcA"
                     /locus_tag="JNZ73_003279"
     CDS             complement(7875..8813)
                     /gene="tdcA"
                     /locus_tag="JNZ73_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HAY8733458.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            9003..9347
                     /gene="tdcR"
                     /locus_tag="JNZ73_003280"
     CDS             9003..9347
                     /gene="tdcR"
                     /locus_tag="JNZ73_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HAY8733459.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            9603..10142
                     /locus_tag="JNZ73_003281"
     CDS             9603..10142
                     /locus_tag="JNZ73_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733460.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            10164..11340
                     /locus_tag="JNZ73_003282"
                     /pseudo
     CDS             10164..11340
                     /locus_tag="JNZ73_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
BASE COUNT         3315 a         2846 c         2548 g         3028 t
ORIGIN      
        1 ttgtgcaccc aaggatgaaa gctgacagca atgtcagccg cagaccactt taatggccag
       61 tcctccgcgt gatgtttcgc ggtatttatc gttcatatct ttgccggttt catacatcgt
      121 ctcgatcact ttatcgagtg aaacacgcgg tgccgaggtg cggcgcatcg ccatccgcgc
      181 ggcgtttact gctttcacgg cattaatggc attacgttca atgcacggga tttgtacctg
      241 tccggcaacc ggatcgcagg tcagcccaag gttatgctcc atcgcgattt ccgccgcatt
      301 gcatacctgc gccggactgc cgcccagtag ttcagttaac cctgccgctg ccattgaaca
      361 ggccacgcca atctccccct gacagccgac ctccgcgcca gagatggagg cgttcatttt
      421 atacagcgcg ccaatagccc ccgctgccag aaaatagcgg gcaattgacc gctcgtttac
      481 cggacgacgg aacttatcgt aataagccag cactgccgga ataatgccgc acgcaccgtt
      541 agtcggtgcc gttaccacgc gcccgccagc tgcgttttct tcactaaccg ccagcgcgta
      601 catgttgatc cagtcgatga cattcatcgg atcgttagag atgttatcgc tagaaaccag
      661 ctgacgatgc agcgctacgg cacgacgcgg cacattgagc ggaccaggca gcacgccttc
      721 agtgttcatc ccgcgttcaa taccgtcatg catcacttgc cagatacggg caaaccctgc
      781 gtcaatttcc gctttgctgc gcagcgccag ctcgttgtgc atcatcaggc cagatataga
      841 caggccgttg taatcacaca ttttcagcag ttcacctgct gagtggaaat cgtaaggtac
      901 ggacgtttcg acatcgtgcg acaggccgaa gtgttcttct tcgacaataa acccgccgcc
      961 gacagagtaa taggttttac ttaatagcgc ttcctgtccc ttccaggcag tgatccgcat
     1021 tccgttctca tggcgaggca acatttcggg atggaagata atgttctttg ctacaggaaa
     1081 atcaacaata tgcgcaccag atgccactgg cagccgcccg ctgcgcgtta ctaactctat
     1141 aaatgcaggg atctcatcaa tgacaacatc ctgcggactg tttcctgcca gtcccatgat
     1201 aatggcgaca tccgtggcat ggcctttgcc cgtcagtgac aacgacccgt acagatcgac
     1261 cacaatatgg ctcgtcgcgg ttaataagcc gctactttcc agccgatcaa taaaactttt
     1321 tccggcattc attggcccca cggtatgcga actggaggga ccaatcccaa ttttgaaaat
     1381 atcgaatgca ctaatcatat ccacaccctc ggattgccgt tcagtgaagt ggagcggaac
     1441 gaccttgcga ccgtcccgct cacgaggctt tacgcactac gtactgcgat agcttcaatt
     1501 tccagcttca catctttcgg caaacgcgca acctgcacac agctccgggt cgggtaggtc
     1561 gcctgatgct catcgaagaa ctgcttatag acttcattga tggtggcaaa atcattcaga
     1621 tcggtgatga acacggtcat cttgatgata tcgcccacgc tcagcccggc agcaaccacg
     1681 atcgctttga cgttttcgag gcttaaacgc gcctgatctt gcacatcagc cgggatctca
     1741 ccggtctgcg ggcaaaccgg aatttgcccg gaggtgaaga ccatgctgcc taaatcaacg
     1801 ccctgaacat aagggccgat tgcgcctagg gcacgttgcg tttcgataat ctttttcata
     1861 catcctccgg cgtcagagcg cctgggtaaa agtacgtgaa ataacatcct gttgctgttc
     1921 acgggtcagt gcgttgaagc gcacggcgta gccagagaca cggattgtca ggttaggata
     1981 tttttccggg tgctcgatgg catccagcag catttcccga ttcattacgt tgacgttgag
     2041 gtgttgaccg ccttcgacat ccgcttcgtg gtggaaatac ccatccagca ggccgacaag
     2101 gttggtttta cgtactggat cttctttgcc cagcgccgca ggaacgattg agaaggtgta
     2161 cgagatccca tctttggcgt aggtgaacgg cagtttcgcc accgacgtca atgaggccac
     2221 ggcacctttg cggtcacgac catgcatcgg gttagcgccc ggcgcgaacg gtgttccggc
     2281 gcgacgaccg tccggcgtat taccggtttt ctggccgtac accacgttag aagtgatagt
     2341 cagaatcgac tgggtaggga cggcgttgcg ataggttggc agcgctttaa ttttcttcat
     2401 aaagcgttca accaggtcgc aggcaatgct gtctacgcgc tcgtcgttgt tgccgtactg
     2461 cggatattca ccgtcgattt caaagtccac cgccaggccg ttttcgtcac ggattggttt
     2521 cacgcgggca tatttgatgg cagacaggga gtccgtcgcc accgacaggc ccgcgatgcc
     2581 gcatgccata gtgcgataga catcacgatc gtgcagcgcc atcagcgaag cttcgtagct
     2641 gtacttgtcg tgcatgtagt gaatgatatt cagcgcgctg atgtactgca ccgccagcca
     2701 gtccatgaag tgatcgaggc tgtccatcac tttgtcgtaa tccagcacgt cgtccatcag
     2761 cggtgctgtt ttcggcccga cctgaatctt cagcttctcg tccaccccgc cgttaattgc
     2821 gtagagcagc gttttcgcca ggttagcgcg tgcaccaaag aactgcattt gcttaccaat
     2881 caccattggg ctgacgcagc aggcaatcgc gtaatcgtcg ctgttgaagt cagtacgcat
     2941 cagatcgtca ttttcatact gcaaggaaga ggtgacgatc gacacctgcg cggcatattt
     3001 tttgaaggca atcggtaatt cttccgacca aagaatggtc aggttaggtt ccggtgccgg
     3061 ccccatagtg tgcaggtgtg caaatagcgg aaggagtttt tggtcaccag cgtacgaccg
     3121 tccagcccca tcccgccgat cacttccgtc gcccagattg ggtcgccgga gaacagcgaa
     3181 tcaaattccg gtgtacgcag gaagcgtacc atacggatct tcatgatgaa gtgatcgatc
     3241 agttcctgtg cctgctgctc attgaggacg ccagctttaa agtcgcgctc aatgtagata
     3301 tccaggaacg atgccgtgcg ccccagcgac atcgcgccgc cattttgcga tttcactgcc
     3361 gccagataag cgaagtagag ccactgcacc gcttcctgtg cattctgcgc cgggcgagag
     3421 atatcaaagc catatttcgc cgccatttcc tgaatctgca acagcgcatg acgatgctct
     3481 gccagctcct cacgcagacg gatggtggct tccagatcct cgcctttttc cagacgagac
     3541 tggagatcgg caaattgcag ttcgcgttca cgcaccagat aactgatgcc atacagcgct
     3601 acgcggcgat agtcaccgat aatgcgccca cgaccatagc catctggtaa accggtcagc
     3661 acgccagatt tacggcagcg cagcatatcc ggtgagtaaa catcaaatac gccctggtta
     3721 tgggttttac gcagatcggt aaacagatat tcaaattcac tgtccatttc acggccatag
     3781 gcgtggaatg aacttttaat catattaatg ccaccgaacg ggtgtagcgc acgtttcaac
     3841 ggcgcatccg tttgcaggcc aacaattttt tccagcggct ggttaatata tcccgcatca
     3901 tgagcggtaa ttgtggtggc aatattggta tcgaaatcaa ccggcgcgtg ggttgcgttt
     3961 tcgatacgga tgccttccat tactttttct cacaattccg tggtggcagg cgtcgcttcg
     4021 gcgagaaaag attcatcgcc ttcatacggt gtatagttat gttgaataaa atcgcggaca
     4081 ttaatttcgc ttttccagtc cgtaccttta aagccaagcc atgcgtcagc gtacagctta
     4141 tcgctggtat caatatctac cttcatgaaa aataatctct ctacaatact tcaactaaat
     4201 tatgcaaatt ctgcgggcgc gttaacttta cctaaatgaa tggcatccaa agcaatcatt
     4261 ttttcttcgt tagtcggaat aacgacgcaa atgacatgcg cattttcact ggaaacaatt
     4321 cgctcgccaa aagagttaga tcgattattc atttctgtat caatctctac gcctaataca
     4381 gccaaatgtt ccatgaccag acgacgaatt aaacttgaat tctctcctat tccgccggtg
     4441 aatataattc catccagccg acgtaatgaa gctgcatgtc cggcaatatg acgggcaatt
     4501 cggtgaacaa aggttttaat tgccagttgc gcgcgttcgt gaccttcatg ccaggctttt
     4561 tccagaacac gtaaatccga agaaagaccg gaaataccta ataatcccga ctctttattc
     4621 actacgcgtt ccaggtcacc caggctctgg ttggtttggc tggcgaccca ggacatcgca
     4681 ccaaagtcga catcgccact gcgggtaccc atcatcaagc cttccagcgg cgtcattccc
     4741 attgaggtgt caacactctg accgttgcga actgcgcaga ttgacgcgcc attgccaaga
     4801 tgcgccacaa ccaggccgga gtcatcttcc gccagattca gcagcgaatg tgcgcgctgg
     4861 gaaacatagc ggtgcgacgt gccgtggaaa ccataacggc gtacacctaa ctcttcataa
     4921 tatttccacg gcaggccgta taaataagct tccggagcca tcgtctggtg gaaactggta
     4981 tcaaatacgg ccacctgagt tacgcccgga aataattgct gcgccgattc aataccactt
     5041 aaatttgcgt aattatgcag gggtgccagt ggagaaacgc gacggatatt atcaatgact
     5101 tcatcggtaa taatggcgga ctcggtaaaa atactgccgc cgtgagcgat gcggtggcca
     5161 attaaggcca cgctgtcatt taaattccgt ttttccagtt caaatgcaat tgccttcaat
     5221 gcaccttcgt agctgtggag agccagcggt gctggctctc ccccatttac ggataagaat
     5281 gcattttccg agttaatacc gtcggcaata cctgacatta atacttcaca gtcgctggca
     5341 tcgagtacgg aaaacttaat cgaagacgaa ccacagttaa taaccaaaac aaccggaaat
     5401 tcattcatct cttttctcat cctgagttac ggattaaaac agtttgtata cgatgttcag
     5461 gatggtcagc agaccaatca cggtaacaaa cacgttatcc agacgaccac ggtatttcgc
     5521 cagagacggc gctttacgga tagcatacat cggcaacagg cacagcaggg atgcgataat
     5581 cggtgcgccc atggcttcaa tcaggtcgag gatgttcggg ttggcgtagg caacaaccca
     5641 ggtggagccc atgatgaaga tcatgctgag agtattcagt ttacccagcg acactttggt
     5701 tttgtcacct ttataaccga acttcagaat cagaccattc aagccttcca gcgtccccag
     5761 atagtgaccg aagaaagatt tgaagatagc cacgagtgcg atgatggaag ccgcatattc
     5821 cagtgtaatc gcgaacgttg ttttcgtacc ggccatggac gcaaagtggt tagccagata
     5881 agaaagcact ggaatattct gcactttggc ttccgccatg ttggccggag acagagtaaa
     5941 caggcagcta aaggcaaaga acatcaccac tgcaaccatc agcatgctgg cacgagaaat
     6001 gatttgggaa catttacgtt cggtgaagtc gcgaccgaag tctttctcat actcttcacg
     6061 tttagaaacc acgaaggaag agacgattgg cgagaagtta aaggagaaaa ccatgatgga
     6121 aatccccagc cagacagtga tcaggatacc gtcatgaccg gttaacgaca gcgaaccgag
     6181 gtcaacctgg tcgataactg cagagttcca gtaagggatc agcgacagag aaatcagcac
     6241 caggctggcg ataaacggcc ataccaggta gctcatcact ttaaccatca gatccttacc
     6301 aaaccagatg acgaaagcca tcagcagcaa caggaacagc gccacaaagc cgcgattcag
     6361 cggtgcaaat ccgagctggt tttcccagaa cgtcataaag gtattggtaa tagtaacgcc
     6421 ataaatccac agcagtgggc aaatcgcgaa gaagtacagg aacgtgataa ccacgccgcc
     6481 agttttacca aaatgctctt ccaccgtttc cgtaatgttg ccggaagggt tagagccaga
     6541 aagacacaga cgcgccagcg cccggtggca ataaaatgcg atggggtatg ccaataccaa
     6601 catcagaaga atcgggatca gtccgccaaa acctgcgcgg atagggaaga acagcacccc
     6661 ggcgccgatt gccgtaccaa acaagcctaa cgtccatgtg gtatctgatt tacgccagga
     6721 cgattgtttt gtctggctgg atacaatgct atctgaagta ctcatatcct atcctcaacg
     6781 aattaattaa gcgtcaacaa aaccggtgat ttgagagacg cgagaaagat cgatattgcc
     6841 gccggaaata atactgacgg tttttctgtt ttgaatatat tggtctaatt taccgcttaa
     6901 taatgcagca catgccagag cgcctgcgcc ttcggtgacg actttattgc gctgaattaa
     6961 ggcaatcata ctgtttctga tttcgtcttc gctgaccagc acgatgtcat cgactaattc
     7021 acgaacgatt tcgtaagtta aattacccgg gcgggagaca tcacaaccat ccgccagggt
     7081 gccggtagtt cggtgcgtgg ttatttctcc ggagtggaaa gaagccgcca tgccgtgaac
     7141 gttttctgac tgtacgccaa taacacgaat ggtcgggtta atagatttaa ttgccaccgc
     7201 aataccagca attaaaccac caccaccaat tggcacaatc acgttatcga catcatagag
     7261 atcttccata atttccagac caatcgttcc ctggccagca atcactttcg gatcatcgta
     7321 aggtgggata aaaatacgac cttccatttc gacaatttcg ctcactttag cgatagtgtc
     7381 gttgaagtta tcaccatgca gaacgacttc tgcggagtag tcgcacgttg ccgctacttt
     7441 ggattttggc gcaccttttg gcatcaccac tttaccgtcg atacccagta tcgcgcagga
     7501 gagcgaaacc ccttgcgcat ggttgcccgc agaacaggcc accacgcctt tgcgtttttc
     7561 cgcatcggtc agtgaactta atttattaaa tgcgccacga attttaaatg aacccgtacg
     7621 ctgcatattt tcaaacttca ggaatatttc acctttgcaa cgttcactaa aatagttgga
     7681 gcgaggcatg cctgttttat aaattcgccc agccagtcgt tgtttcgctt caataatatc
     7741 atcaatagca accggcagat cgtatgtaat atgcattata aaacctcttc gcctgaatta
     7801 aatatgtagg taaccgtaac cgacacctgc aaaacagaca ggtggattat ttatgtttaa
     7861 ataaaaacaa ataactaacc aacttctatt aattgccttc gtctacaccc attataagat
     7921 gaatactctt tggctaattc caccaaaacc gatgctgctt ttttaatacg ataatttttc
     7981 gaccataccg cggcatattg tgccacaggt aatgtttctt caaccggaat agtaataaat
     8041 tgattagaac caaaaggtga cgtcatatca caaggaatta cagttaagaa atcagcattg
     8101 agaacaagat tataaattgt cacgactgag tcggttttaa cgatgttttc aatactgatg
     8161 ccatttcttt gtaacgtagt aagcagttcg ctgtagtacc ccatattagt ttgtggcaac
     8221 acccactgtt cgttcttcaa cgactccagc gtggtggtgc cggtgcatgt tcgggactta
     8281 ctggctacca gcacaaactc ggactcgaac agcggctcaa catgtaaatc ctgaagcttc
     8341 atttctgcac ttaacgtacc aatcgcaaaa tccagtcgac cgtcgcggat tgccggtaag
     8401 aacgaagaca gttgcgcttc atacatagaa acctgcgctt tcgggaacac ctctttgaac
     8461 ttgttgatca tccctgacat aaaagtaaaa ccaatcaatg aaggaaaacc aaatgagact
     8521 tccaccaccg cctcagaaga cataccgctt atctcattaa ccatattttt catttcacgg
     8581 gtaatggatt cggaacggga gagtaacaat tgaccggcag gtgttaatgt tacaccggta
     8641 tttttccgca ccactaattc cacaccaaaa taatcttcaa tatcgttaat gattttactg
     8701 acggccggtt gagttaaccc taattctttt gcagccgagc cgatagagcc acttctaatg
     8761 acttcctgaa agactaccag gtgctgcgtt ttcggaagaa gaatagtgct cataacgacc
     8821 tacgttaatt acctcattga cggcatgaag tgtatcaaaa tgaaataaac aggatatgtg
     8881 cgaccactca caaattaact ttcattactt tccaaggtat cgttattaca attaaatacc
     8941 tttaaatatc aacaagttaa agcataaaaa tcagcataaa ccctgatttc tatcaaaaaa
     9001 aaatgacggg gataaccata ttttatggcg ataacatcat tcgttatgtg gttaatacaa
     9061 aaaaggggct gagaccatat ttcaagcaat taccggataa ttaccaggca aaatttgagt
     9121 taaatttaat gagcaagttc tcaaatttta ttataaataa accattttca gcgataaata
     9181 ctgcggcacg tcacattttt tcacgctatt tgttggagaa caaacattta ttttatcaat
     9241 attttaaaat ttcgaataca ggtattgatc atctcgaaca attgattaac gtcaactttt
     9301 tctcttctga caggacgtca ttttgtgaat gcaatcgttt tccataaatt cttctcccct
     9361 cataggcgac gaatagcatt ttgtgttgag gatcacaaaa cgaataattg ctggtcgccg
     9421 cgataaggtc agacgaagtc aacaaggggg attttcactg tgctttacat cagcgtagga
     9481 cacagaatgt tgcttcgttt actgtaacgc cgggtaaatg agcgtttttt gatagtgcgt
     9541 aagtgtcgtg cgaggaaaat aagcatctat tattgtttgt catgacgaca aggcaggcat
     9601 caatgaaagg cttcccaatt gcacatattt ttcacccttc aatcccgcca atgcacgcag
     9661 tggttaacaa tcacaataga aatattgatt attggacggt aaaaagaaag tttgcagaaa
     9721 ttgtctccac caatgacgtt aataaaattt acagtataag taatgaactg cggagagtat
     9781 tatctgcaat tactgcattg aatttctatc aaggcgatgt tccttctgtc atgatccgaa
     9841 tccaaccgga aaatatgagt ccattcatca tagatatttc tacaggagaa catgatgatt
     9901 atatcataca aacattagat gtaggcactt ttgcaccttt tggtgaacaa tgtacttgct
     9961 cagccgtcaa taaaaaagag ctggaatgta ttaaggagac gatttctaag tattgcgcaa
    10021 agttcaccca aaaagaagcc atcttaacac cccctaggca ctttaataaa acctgcatta
    10081 cttcagattg ttggcaaatt ctcttttttt cacccgatca ttttaataat gatttttatt
    10141 gattatttat aaaggataaa ataatgttcc ctgtttcatc aattggtaat gatattagta
    10201 gtgatttagt ccgtcgaaaa atgaatgacc ttcctgaaag ccccatagga aataacctcg
    10261 aagcactagc acccggtata gaaaaactaa aacagacctc tattcaaatg gtcactttac
    10321 ttaacgcgtt acaaccaagt ggaaaatgca ttatcactgg tgattttcaa aaagaattag
    10381 cgtacttaca aaatgtaatt ctttataatg actcgtctct tcgtctggat tttttaggtt
    10441 ataacgccaa aattattcaa cgatcggaca atacctgcga acttaccatt aatgaaccgt
    10501 taaaaaacca ggaaatatct acaggcaata tcaatgttaa ttgcccatta aaagatattt
    10561 acaatgaaat caggaggttg aacgtaatct ttagttgtgg gactggagat atagttgatc
    10621 tatcctctct ggacttacgt aatgtcgatt tagattatta tgatttcaca gataaacata
    10681 tggctaatac tattctaaat cattttaaat tggattcaac aaattttact aatgccaaca
    10741 tgtttcaggt taattttgtt agttcaacac aaaacgccac aatctcctgg gattatttac
    10801 taaaaataac gcctgtttta ataagcatta gcgatatgta ttctgaagaa aaaatcaagt
    10861 ttgtcgaaag ttgtttaaat gagcttggag acattaccga agaacaatta aaaattatga
    10921 gatttgcaat tataaaatct ataccaaggg caactcttac agataaatta gaaaatgaat
    10981 taacaaaaga aatatataaa agctcatcga aaatcaacaa ttgcttgaac agaattaaat
    11041 taccagagat gaaagaattc tcatcaaaaa aatatatgat tacatcgata caatcattga
    11101 agattatgaa aatatcaaag aaaatgctta tctggtcgtc ccccaaatta attatactat
    11161 ggatttaaac atagaagact ctagctcaga agagttaata ccctcgagaa agacgaaaat
    11221 tctccggaca atggctttga ggtcggggaa tataacacat atgaagcata taactcagag
    11281 aatcaatatt ttaccagaga ggactatacg tataattacg accttttaaa tgcaatatag
    11341 acttaataac ttcttacaaa gtctattatt aataccgtag atattcatct taccctgtgc
    11401 accaaaagca aaaacttatc gacgcgggtt tgaaactgat ggcaaccgca aaaatgcctg
    11461 atgcgctacg cttatcaggc ctacgccatc tctgcaatat attgaatttg cgtgcttttg
    11521 taggcaggat aaggcgttca cgcagcatcc ggcatgaata aagcgcactc aacaatctga
    11581 aacccgccgg aacggtttat tacgtacatc aggtgaaact gaccgataag ccgctttctt
    11641 ttgggtatag tgtcgtggac agtcattcat ctttctgccc ctccaaaagc aaaaacccgc
    11701 cgaagcgggt ttttacgtat atcaggtgaa actgacc
//
LOCUS       DADMGC010000122        11553 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.122, whole genome shotgun sequence.
ACCESSION   DADMGC010000122 DADMGC010000000
VERSION     DADMGC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.122"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="JNZ73_003283"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="JNZ73_003283"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1564)
                     /gene="garK"
                     /locus_tag="JNZ73_003284"
     CDS             complement(413..1564)
                     /gene="garK"
                     /locus_tag="JNZ73_003284"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HAY8733461.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHTWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGAGSLTDDVGV
                     VHQHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1661..2551)
                     /gene="garR"
                     /locus_tag="JNZ73_003285"
     CDS             complement(1661..2551)
                     /gene="garR"
                     /locus_tag="JNZ73_003285"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAY8733462.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2581..3351)
                     /gene="garL"
                     /locus_tag="JNZ73_003286"
     CDS             complement(2581..3351)
                     /gene="garL"
                     /locus_tag="JNZ73_003286"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HAY8733463.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATHKLADTFKK"
     gene            complement(3367..4701)
                     /locus_tag="JNZ73_003287"
     CDS             complement(3367..4701)
                     /locus_tag="JNZ73_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8733464.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5076..6647
                     /gene="garD"
                     /locus_tag="JNZ73_003288"
     CDS             5076..6647
                     /gene="garD"
                     /locus_tag="JNZ73_003288"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HAY8733465.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6699..7523)
                     /gene="agaR"
                     /locus_tag="JNZ73_003289"
     CDS             complement(6699..7523)
                     /gene="agaR"
                     /locus_tag="JNZ73_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HAY8733466.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7772..8572
                     /locus_tag="JNZ73_003290"
                     /pseudo
     CDS             7772..8572
                     /locus_tag="JNZ73_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="with KbaY catalyzes the formation of
                     dihydroxyacetone phosphate and
                     D-glyceraldehyde-3-phosphate from
                     tagatose-1,6-bisphosphate; subunit Z may act as a
                     chaperone for the Y subunit; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
     gene            8572..9036
                     /gene="agaV"
                     /locus_tag="JNZ73_003291"
     CDS             8572..9036
                     /gene="agaV"
                     /locus_tag="JNZ73_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HAY8733467.1"
                     /translation="MVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQQNL
                     MEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRINVG
                     NMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9047..9826
                     /locus_tag="JNZ73_003292"
     CDS             9047..9826
                     /locus_tag="JNZ73_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HAY8733468.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGTEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            9816..10625
                     /locus_tag="JNZ73_003293"
     CDS             9816..10625
                     /locus_tag="JNZ73_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAY8733469.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            10712..11146
                     /locus_tag="JNZ73_003294"
     CDS             10712..11146
                     /locus_tag="JNZ73_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY8733470.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEEYPVEEGI"
     gene            11143..>11553
                     /locus_tag="JNZ73_003295"
     CDS             11143..>11553
                     /locus_tag="JNZ73_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY8733471.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAH"
BASE COUNT         2755 a         3092 c         2952 g         2754 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccgccc gtctcccccg aagaggacga cgacaaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcacccgcaa
      601 tgccaataac cggtttatgg tacttcttcg ccacgtttgc gacgccaatc ggtaccttgc
      661 cgtgaatact ctggctgtca atacgccctt caccggtgat caccagcgta caatcgtgaa
      721 tatgttcctc cagattcagc gccgtagtga cgatttcaat accacttttc agttccgcac
      781 caagaaacgc catcagcgcc gcacccatac cgcccgcagc tcctgcgccg gggacatctt
      841 ttacatcaac atgcagcgct tttttaatga catcggcata gtgagagagg ttattgtcca
      901 gctcaacaat catcgcttca ctggctccct tttgtgggcc aaagatgcgc gatgcgccgt
      961 tatcgcccac cagcggattg gtgacatcac aagcgacgcg aatgacgcaa tcttttaagc
     1021 gcggatcgag gccggaaata tcaatatcat tcagagtatt aagactaccg ccgccaaaac
     1081 caatttcatt gccgttggcg tcgcataatt tcgcccccag cgcctgtacc atgcctgcgc
     1141 cgccatcatt tgtagcgcta ccgccaatgc cgataataat gtttgtcgcg ccgctctcca
     1201 gcgcctgcag gattaactcg cctgtgccgc gtgaagtggt cacgagtgga tcgcgttttt
     1261 ccgcaggtac cagctccagc ccactggccg ccgccatttc aataaacgcg gttttgccat
     1321 cgccggagat cccccaactg gcattcactt tctcgcccag cggccctgta acccaggtgt
     1381 gacgttcaga accctgggtg gctgcaatca tcgcttccac cgttccttcg ccaccgtcgg
     1441 caaccggaat agaaacgtac tgtgcatcag gaaaaatttc ccgaaatcct ttttctatcg
     1501 cctgcgcaac ctcgctggca gataaacttt ctttataaga gtctggggcg attacgattt
     1561 tcatacctat gcctgttacc acatgacgcc ggagggcgtt tctcttattc ggcctggatt
     1621 ccaggcccgg attgcaatac gccatccggg cacgacgtca ttaacgagta acttcgactt
     1681 tcgccagttt ttcgtagtag cacgccaggg cgctatgatc cgccgttcct aaaccatctg
     1741 ctcgcagtgc ctgcatcatc tccataaccg cagctgtgag cggcagttgc gcgccgacgc
     1801 cgtgggaagt atccagcgca tttgccagat ccttaatatg cagatcaata cggaagcccg
     1861 gcttgaagtt gcggtccatc accatcggcg ctttggcatc cagcacggta ctgcccgcca
     1921 gtccaccgcg aattgcctga tacaccaggt ccgggttaac gcccgcttta gttgccagcg
     1981 ttaacgcttc tgacatcgcg gcaatattca gcgccacaat gacctgattc gccagtttgg
     2041 tgacgttacc tgcaccaatt gccccggtat gcaccacgga acccgccatt gctttcatca
     2101 aatcatagta tttgtcgaaa atagccttgt cgccgcccac catcactgac agcgtaccgt
     2161 cgatggcttt cggttcaccg ccgctcaccg gagcatccag catatcaatg cctttcgctt
     2221 tcagcgcttc gctgatttca cggcttgcca gcggtgcgat agaactcata tcgatcaata
     2281 ccgtacctgg cttcgcgcct tcaataatgc cattctcacc cagcgccacc tctttcacat
     2341 gaggggagtt tggcagcatg gttatgatga cgtcgcactg ttcagcgatc gctttagccg
     2401 tagaggctgt ttctgcacct gcagcaatca cgtcagcaat agcttctggg ttacggtcag
     2461 caaccaccag cgagtaacct gctttcagaa ggtttttact cattggttta cccataatcc
     2521 ctaggccaat aaaaccaact ttcatagtca tatcaatcat ctctcttgtt gcggtggtgg
     2581 ttatttttta aaggtatcag ccagtttatg agtggcagag cggaagacgc cgagatcgct
     2641 gccgacagcc acaaacgtcg cgccccattc cagataacga cgcgcatcgg cttcgaccgg
     2701 cgcgaggata ccgctgggtt tgccgtgcgc gctggcacgg ttaaaaatgt gctgaattgc
     2761 tttttgtaca tccgggtgtg atgcattgcc gagatggcct aatgccgcgg ccagatcgct
     2821 ggggccgacg aagatgccgt ctacgccttc ggtagcggca atggcatcga cgttatctac
     2881 gccctgctga ctttctatct ggaccagaat agtgatgttc ttgttcgact gagcgaaata
     2941 atccgccacg gtgccaaaca tattggcgcg gtgagaaacg gagacgccgc gaatgccttc
     3001 cggtgggtaa cgggttgatg ccaccgcctg ctctgcttcc tcttttgttt ctacaaaagg
     3061 aatcaggaag ttatagaaac cgatatccag aagacgctta ataattaccg gctcgttggt
     3121 cggcactcgc actactggcg cgctggcgct gcctttcaag gccattaact gcggaataaa
     3181 cgtggagata tcgtttggcg catgttcgcc atccagcacc agccagtcaa acccagccaa
     3241 accaagaact tcagtgctaa tcgggttaga gagtgctgac cagcaaccaa tttgtacctg
     3301 tttcgcagcc agtgcggctt tgaatttatt cgggaaaaca tcgttattca tcgcttatac
     3361 ctttgcttat ttctgcaatt ccatacgttt aatgtcgcca actacgaaga ggtagcagac
     3421 catcgccatc agcgctgaac atcccacgaa aaccagtgct gcattgaagg agtgcagttc
     3481 acttaccagg tagccaatca ccagtggagt gacaatggag gcaacattgc caaagacgtt
     3541 aaagacgccg ccacagaggc caacaatctc tttcggcgcg gtgtcagaaa tcaccggcca
     3601 gcccagagca ccaaatcctt tgccaaaaaa agccagcgcc atcagcatga ccaccagcgt
     3661 ggtgttgttg gtgtagttac ataagatgat ggtggaagcc agcaacattc ccagcacaat
     3721 cggtagctta cgtgccaggg tcagggataa accgcgtttg atcagataat ccgagaagac
     3781 acctcccagc acgccgcccg caaaaccaca cagtgccgga atcgaggcga ccagacccac
     3841 tttcagaatc gacatgcctt tttcctgcac cagataaatc gggaaccagg tgaggaagaa
     3901 ccaggtgatg gtgttgataa aatattgtcc gaaaaatacg cccaacatca tgcggttaga
     3961 gagcaattgc ttgatgtaat gcagtttggg accgcttgct gccgcgctgc ccggcttttt
     4021 gtggtccata tcgaccaccg cgccattttc agagataaac ttcagctctt cctcagacat
     4081 acgtgggtga tctgtcgggt tatgaatcaa cttgatccac agcgccgtca gcacaaaacc
     4141 aatcaccccc ataacggtaa agacgtgctc ccatccccag gcgaaagtca gccagccaag
     4201 cagaggcgaa aagagcgcca gcgagaaata ttgtgccgag ttaaagatgg cggaggcagt
     4261 accacgttct ttcgtcggga accaggcggc gacaatacgg gcgttcgccg ggaatgatgg
     4321 agcttccgag aatcccagca taaagcgcat aaagaacatg gagatccctg cccaggccag
     4381 cgggaacata tcaacaaagc cttgcaggaa ggtgaacagc gaccagaaaa agaggctgta
     4441 ggtgtaaact tttttcgagc caaacttatc aagcaaccag ccgccgggga tttgcatcag
     4501 caagtaggcc cagccaaaag cggagaagat gtaacccatc gaaaccgcac ttaactgcaa
     4561 ctcttttgcc acttcggtac cagcaataga cagcgttgca cgatcggcgt agttaacggc
     4621 ggtaacaata aaaataatca gtaatattaa atagcgggta tgcacacctt tccttttttc
     4681 gtcaacggtg tccagaatca ttttatttac ctcgggtact tatgctgatt tttattatta
     4741 tggggaaggt gttatttata agtttcattt atgccgtaac gacaatgaac tcgggaatta
     4801 gtataagcag cgcgagaata ataatcattg tgcaaatgct aatttaatta atacttttta
     4861 aatattattt tgagcatatg cacataaggt tgcgcgctaa agcacagatt tgcgctttac
     4921 cttgccgggc ggcactgcaa tccctgaaat gattgacatt gatcacattt ctgcgtttaa
     4981 actcctgaca ttcttatttc acccaatgaa gtcatttatt tttaaatgag accaggtcct
     5041 cattttaata acccctggct ggagaatatt gcacaatggc caacatcgaa atcagacaag
     5101 aaacgccaac tgcgttttat ataaaagttc acgacacaga taatgtggca attattgtta
     5161 atgataatgg cctgaaagca ggaacgcgtt ttccggatgg gctggaatta attgaacata
     5221 ttccccaggg gcataaagtc gcattgctgg acattccggc taatggtgaa attattcgtt
     5281 atggcgaagt gattggttac gccgtgcgtg caatcccacg cggaagctgg atcgacgaat
     5341 caatggttgt actaccggaa gcgccgccgt tacacacgct gccactggca accaaagtcc
     5401 cggaaccctt accgccgctg gaaggataca cctttgaggg ctatcgcaat gccgatggca
     5461 gcgtgggcac caaaaacctg ctcggcatca ccaccagcgt ccactgtgtg gcaggcgtgg
     5521 tggactatgt agtaaaaatc attgaacgcg atctgctacc gaaatacccg aacgtcgatg
     5581 gcgtggtggg gctgaatcat ttgtacggtt gtggcgtggc gattaacgca ccggcggcag
     5641 ttgtacctat tcgtaccatt cacaatattt cgctgaaccc taactttggc ggcgaagtaa
     5701 tggtgattgg cctgggttgt gaaaagttgc agcctgagcg cctgctgact ggaacggatg
     5761 atgtgcaagc tattccagta gaaagcgcca gcattgtcag tttgcaggat gaaaagcatg
     5821 tcggttttca gtccatggtc gaggatattt tgcaggtcgc cgaacgccat ctacaaaaac
     5881 tgaatcaacg tcagcgagaa acctgtccgg cttcagaact ggttgtcggc atgcagtgcg
     5941 gtggcagcga tgcgttttct ggcgtaacgg caaacccggc ggttggctat gcgtctgatc
     6001 tactggtgcg ctgcggcgca acggtgatgt tttcagaagt cacggaagtg cgtgacgcga
     6061 tccatctgct gacaccacgc gcagtgaacg aggaggtcgg caaacggctg ctggaggaga
     6121 tggagtggta cgataactat ctcaatatgg gaaaaaccga ccgcagcgcc aacccttcgc
     6181 cgggcaacaa gaaaggcggt ctggcaaacg tggtagagaa agcgctcggc tccattgcta
     6241 aatcgggcaa aagtgcaatt gttgaagtgc tgtcgcccgg tcaacgcccg actaaacgcg
     6301 gattaattta cgccgcgacg ccagccagcg attttgtctg tggcacgcaa caggtggctt
     6361 cgggtatcac cgtgcaagtg tttacgaccg gtcgtggtac gccgtacggc ctgatggcgg
     6421 tacccgtcat taaaatggca acccgcaccg agctggcgaa ccgctggttt gatttaatgg
     6481 atattaatgc gggcaccatc gctaccggcg aagaaactat tgaagaggtg ggctggaagt
     6541 tgttccactt tattctcgac gtcgccagcg ggaagaagaa aaccttctcg gatcaatggg
     6601 gactgcataa ccagctggcg gtgtttaacc cagcaccggt gacctgataa ttcaattcgt
     6661 attatgcccg atagcacacg tttatcgggc atattgagtc acagcgaaga cgcgtgatcc
     6721 ccgaccagaa tcacttcaac cccagccttt cgcagtcctt ccagactatc cgcaggaatg
     6781 ccttcatcaa caatgatcat gtcgatacgt tgagtatcaa tgatcttatg taaactggaa
     6841 cgattgaatt tactggaatc ggtgaccacg atgatccgtt ccgcaacttc gcacatccga
     6901 cggtttaaac gggcttcatc ttcattatgt gtgctgacgc cgcgctccag atcgatcgca
     6961 tctacaccaa gaaacagcat atcgaagtgg taattttgca gcgattgctc agcctgatcg
     7021 ccgtaaaaag attgcgactg acggcgcaaa tgcccgccgg tcatcagcag ctcaacgcct
     7081 tccgcttcca gcaacgcatt agccacgttc ataccgttgg tcatcgcaat tacgtcagtg
     7141 tacttgcgca tcagacgagc aatctcaaaa gtggtggtcc cggaatcgag gatcacccga
     7201 tgacctggct gaatcaactc aacggcagct ttcgcaacgc tgcgtttcat cgcggtgttc
     7261 agtgcgcttt tatcttccac tgatggctcg actgacggcg tcgtgctatc gcagatcaac
     7321 gcgccaccat aggcacgcac agcgatcccc tgcttttcca gaaaagccag atcattgcgg
     7381 atcgtcacag tagatacgcc atacaatgcc gacagatcgt taacctgcac actcccttgc
     7441 tgtcgcagac gctgaatgat ctgttctcgt cgctcgctgg tgcctgtcac tcgcttctca
     7501 cctgaagcgt cggtattact catagtaagt cctttcgtaa aactttcgtt tcatttcgtt
     7561 ttgcctatta acgcctttct attaagcaaa tgcaagccca tcttgcccat tggcgcaagc
     7621 tactctcgtt tcagtgactt tcattatgtt tcttttgtga atcagatcag aaaactatta
     7681 tatttcgttt tatttttatc tcaccatgat gcagtattaa ttgaaacaaa acgaaagatt
     7741 aatatcgcag taatctgaac tggagaggaa agtgaaacat ctgacagaaa tggtgagaca
     7801 gcacaaagcg ggcaaaacaa atgcaattta tgccgtttgt tccgcacatc cgctggtgct
     7861 ggaggctgca atccgctacg ccagtgcaaa ccaaacgccg ttactgattg aagcaacctc
     7921 caatcaggta gaccagttcg gcggttatac cggaatgacg cccgccgatt ttcgcggctt
     7981 tgtttgtcag ctcgccgact cgttgaattt cccgcaggat gcgctgattc tgggtggtga
     8041 ccatctgggg ccaaaccgct ggcaaaacct gccagccgct caggcaatgg ccaatgccga
     8101 tgatttgatt aaaagctacg ttgcggcagg attcaaaaaa atccaccttg attgcagcat
     8161 gtcctgtcag gacgatccaa ttcccttaac tgatgacatc gtggctgaac gcgccgcccg
     8221 tctggcgaaa gtggcggaag aaacctgtcg cgaacacttt ggcgaagccg atctggagta
     8281 tgtcattggt accgaagtgc cggtacctgg cggcgcgcat gaaaccttaa gcgagctggc
     8341 ggtcaccacg ccggatgccg cccgcgccac gctggaagcc catcgtcacg cttttgaaaa
     8401 gcaaggtttg aatgccatct ggccacgcat cattgccctg gtggttcaac ccggcgtcga
     8461 attcgatcac accaacgtta ttgattatca gcccgccaaa gcgagcgcct taagccagat
     8521 ggtcgaaaac tacgaaacgc tgattttcga agcgcactct accgattatc aattgtttta
     8581 agccggattg atgaacgctt gattcacggt caggtcggcg ttcagtgggt cggatttgcg
     8641 ggggcaaatc tggtgctggt agccaacgat gaggttgccg aagatccggt acaacaaaac
     8701 ctgatggaaa tggtactggc agaagggatc gccgtacgtt tctggacgct gcaaaaagtt
     8761 atcgacaaca ttcatcgcgc cgccgatcga cagaaaatcc tgctggtttg taaaacaccc
     8821 gccgatttcc tgacgctggt gaaaggtggc gttccggtga atcgcattaa cgttggcaat
     8881 atgcactacg ccaatggcaa acaacaaatc gccaaaacgg tttctgtgga tgcgggcgat
     8941 atcgcagcat ttaacgacct gaaagccgct ggggtggaat gcttcgttca gggcgtcccg
     9001 acagagcctg ctgtggacct ctttaaatta ctttgaggga ttcatcatgg aaatcagcct
     9061 gttgcaggca tttgcgttgg gcattatcgc ctttatcgct ggcctagata tgtttaacgg
     9121 cttaacccat atgcaccgcc cggtggtcct cggcccgttg gtcgggctgg tacttggcga
     9181 tctgcatacc ggaattttaa ccggcggtac gctggaacta gtgtggatgg ggctggcccc
     9241 gctggcgggc gcacagccgc ctaacgtgat tatcggtact atcgtcggca cggcgtttgc
     9301 cattactact ggcgtgaaac ccgatgtcgc ggtcggtgtc gccgtacctt tcgctgtcgc
     9361 agtacagatg gggattacct tcctgttctc ggtgatgtct ggcgtgatgt ctcgctgcga
     9421 ccggatggcg gagaacgccg atactcgcgg cattgaacgc gtgaactatc tggcactgct
     9481 ggcactcggc actttctatt ttctctgcgc tttcctgcct atctacttcg gcacggaaca
     9541 tgcaaaaacc atcattgatg tcctgccgca acgattaatc gacggcctcg gcgtcgcggg
     9601 cggcatcatg ccagcaatcg gttttgccgt gctgctgaaa atcatgatga aaaacgtcta
     9661 cataccctac ttcatcctgg gctttgttgc cgcagcctgg ctcaagttac cggtgctggc
     9721 tattgctgcc gccgcgctgg cgatggcgct gatcgatctg ctgcgtaaat ctcctgaacc
     9781 gactcaacct gcggcacaga aagaggaatt tgaagatggc atctaatcaa accaccctgc
     9841 cgaacgtcag cgaaaatgaa gaaacactgc tgactggcgt caatgaaaac gtgtatgaag
     9901 atcggagcat tgccgcggag ctgacgaaaa aagatatcaa ccgcgtcgcc tggcgttcca
     9961 tgctgttaca agcgtctttt aactacgaac gtatgcaggc ttctggctgg ttgtacggtc
    10021 tgctgcctgc tctgaaaaag atccacacta ataaacgcga cctggcgcgc gccatgaagg
    10081 ggcatatggg tttcttcaat acccatccgt ttctggtgac gtttgttatc ggcattatcc
    10141 ttgcgatgga gcgttctaag caggacgtta acagtattca gagcaccaaa attgccgtcg
    10201 gtgcgccgct tggcgggatt ggcgatgcga tgttctggct aacgctactg ccgatttgtg
    10261 gcgggatagg cgcaagcctc gcgctacaag gctctattct tggcgcagtc gtctttattg
    10321 tgctgttcaa cgtggtacac ttggggctgc gttttggtct ggcgcattat gcttaccgca
    10381 tgggcgtggc ggcgattccc cttattaaag cgaataccaa aaaagtcggc catgctgcgt
    10441 caatcgttgg aatgacggta atcggcgcac tggtggcaac ctatgttcgt ttaagcacca
    10501 cgctggaaat cacggcgggc gacgcagtgg ttaagttaca ggctgatgtt atcgacaaac
    10561 tgatgcctgc cttcttaccg ctggtctaca ccctgaccat gttctggctg gtacgccgcg
    10621 gctagagtcc gctgcgcctg attgcggtta ccgtggttct agggatcgtc ggtaaattct
    10681 gccatttcct ttaaatacaa tgaggtatta gatgttaagt attattttga cagggcatgg
    10741 cgggtttgcc agtggcatgg aaaaagcaat gaagcaaatc ctcggcgatc aatcgcagtt
    10801 tattgccatc gattttcccg aaacctcaag cgccgcgctg cttacatcgc agcttgaaga
    10861 ggccatcgcc caactggatt gtgaagacgg cattgttttt ctgacggatt tgctgggcgg
    10921 cacgccgttt cgcgtagctt caacgctggc gatgcaaaaa ccgggctgtg aagtgattac
    10981 cggcaccaat ttgcaactgc tactggagat ggtgctggag cgcgaagggt taagcggtga
    11041 agaattccgt gtacaggcgc tggagtgtgg acaccgaggg ctgaccagtc tggtggacga
    11101 gttaggccgc tgccatgaag aatatccggt cgaggaagga atatgacaca cgttctgcgc
    11161 gccagaaggc tgctgactga agagggatgg ttcgatgacc atcagttgcg tgttgctgac
    11221 ggtgtcatcg cagcaatcga accgattcca gtgggcgtga ctgaacgcga tgcggaactg
    11281 ctctgccccg cttacatcga cacccatgta cacggtggtg cgggcgttga tgtaatggat
    11341 gacgcgccgg atgtactcga caagctggca atgcacaagg cacgcgaagg tgtcggcagt
    11401 tggttaccga ccaccgtaac cgcgccgctt aataccattc atgcggcgct gaaacgtatt
    11461 gctcaacgtt gccaacgcgg cggacctggt gcgcaagtgc tggggagtta tctcgaagga
    11521 ccgtacttca cgccgcagaa taaaggcgcg cat
//
LOCUS       DADMGC010000123        11549 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.123, whole genome shotgun sequence.
ACCESSION   DADMGC010000123 DADMGC010000000
VERSION     DADMGC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.123"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(1..336)
                     /locus_tag="JNZ73_003296"
                     /pseudo
     CDS             complement(1..336)
                     /locus_tag="JNZ73_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
     gene            complement(652..1296)
                     /locus_tag="JNZ73_003297"
     CDS             complement(652..1296)
                     /locus_tag="JNZ73_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733472.1"
                     /translation="MMKQGMANSISLTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            complement(1317..1586)
                     /locus_tag="JNZ73_003298"
     CDS             complement(1317..1586)
                     /locus_tag="JNZ73_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HAY8733473.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPEKLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            complement(1665..2303)
                     /locus_tag="JNZ73_003299"
     CDS             complement(1665..2303)
                     /locus_tag="JNZ73_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAY8733474.1"
                     /translation="MGDSVTPEIIGNMIRQYFSQERPEEETIQALNHLRRVLHEVSPF
                     AHEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            complement(2288..3520)
                     /locus_tag="JNZ73_003300"
     CDS             complement(2288..3520)
                     /locus_tag="JNZ73_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY8733475.1"
                     /translation="MSDTLLTEKILTGENVLRAAIARIEWIFETFPSVCLSFSGGKDS
                     TVLFHLVAEVARRRKRHFSVLFIDWEAQYRCTIEHIQKMREMYHDVTETFYWVALPLT
                     TVNGVSQFQPEWICWEPGVTWVRQPPEEAITDMTYFPFFRYAMTFEEFVPAFSSWFAG
                     NRCGVAVLTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKAR
                     DIWIYNARACAIYNPLYDLMYRADVPLRNMRVCEPFGPEQRKGLWLYHILEPETWARM
                     CERVSGAASGALYANESGAYFALRKRISKPAHHTWRSYAMFLLDVMPERTAEHYRNKI
                     AVYLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRH
                     YERYLQRMKERRKEWGIL"
     gene            complement(4021..6189)
                     /locus_tag="JNZ73_003301"
     CDS             complement(4021..6189)
                     /locus_tag="JNZ73_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAY8733476.1"
                     /translation="MIFPFGLNESQLLAVKRAFSSQISVIEGPPGTGKTQTILNIVAN
                     ILIQNKTVAILSNNNSAVSNVYEKMDKQQLGYVMARLGSTENRQQFFSTSISRSEEVL
                     PDSPSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFS
                     HKYRFSSQKTTDLMAYIHYLSDRRIGFRNRIDLLLNFMILKVKPLMIPERRLALFTSL
                     QLSYYEKNIREKQISLNEYEEVFKKSDFKILLGRLTSWSMLYLKQHLRRNVSTRSSFS
                     AETYRDEFDRFIKRFPIIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCA
                     RNVIVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHY
                     RCHPKIIQFCNKQFYDNALIPLTVDSGEASLSLVITAKGNHTRNFSNLRELESLEGHY
                     WDEESSRGYIAPYNAQVNLAEKVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQH
                     SRNIAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFE
                     SPVISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHS
                     QIALNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQA
                     ERDELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            complement(6943..7989)
                     /locus_tag="JNZ73_003302"
     CDS             complement(6943..7989)
                     /locus_tag="JNZ73_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HAY8733477.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSAGTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
     gene            8664..8855
                     /locus_tag="JNZ73_003303"
     CDS             8664..8855
                     /locus_tag="JNZ73_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY8733478.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMRDGYPHPCM
                     QPMYNFLGKFKPEPREETE"
     gene            complement(8908..9141)
                     /locus_tag="JNZ73_003304"
     CDS             complement(8908..9141)
                     /locus_tag="JNZ73_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733479.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(9237..9860)
                     /locus_tag="JNZ73_003305"
     CDS             complement(9237..9860)
                     /locus_tag="JNZ73_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY8733480.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(9949..10458)
                     /locus_tag="JNZ73_003306"
     CDS             complement(9949..10458)
                     /locus_tag="JNZ73_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733481.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
     gene            10916..11374
                     /locus_tag="JNZ73_003307"
     CDS             10916..11374
                     /locus_tag="JNZ73_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733482.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
BASE COUNT         3231 a         2873 c         2311 g         3134 t
ORIGIN      
        1 tgattctgga aaaggacttt gtggattgtt gctattgcca gtgagattga atgaagtcgt
       61 tttaactcgc tttgaatacg tcttgccccc agatttcgct gagaacgtaa ctccagaatc
      121 agagcgactt cgcaagtgcc ggttttcgtt gatggtgaac gcttggggcg acggctgtgg
      181 ctttccagac cagcgatgcc ctgagccaga tatctgcgcc accattttac gcaatgtggg
      241 ccgagaaatg ccacagcgac ggcatacaaa tcccgcatca ccggacgttt catagagttt
      301 tacccactgt agccgctgct gaatttccct gttcataatt tggcattagt tcagctcaat
      361 cccgacttaa aaagtcccta caggatgttc cggacctgag gcacagttca tatccttatc
      421 cataaaaaat ttagctatgt ctatagcatt aatcttgaca agacgacgtt agggcctggc
      481 gatgaatcat ttcccctgcc agatatcctt tggggtgcac cagaactacc gttttatatc
      541 tatataactg aaacgtccct cgccatcctt catattttga tgtagtaaca ccattcactt
      601 tcatctaccc cccgttttat taacatatag taaaaatcac ctcgccgcat tttattcttt
      661 tgaagtaaaa tcatttacca gttcacttaa agctctcttt tgttcatcat catacagaga
      721 ttgaatccgg aatttaatct cttctgcaac cagtcttgca tcgggctcat aaaatatttt
      781 tgttattcgc tgtttctgat gttcaggctg gtcttccata tttcgtaaaa ccagatgaaa
      841 taatatatca taaagtgcgc aatgccatga ataaccatca ttggttaaaa taagctccca
      901 aacccgctcc tgctcggcaa taagctgcag aacaaaggac tcaacctgtt ctgtttgttc
      961 gggggcaaca cggtatagcg gagcaatcac agcaaaagat agttcattta accaggattc
     1021 cgcttccttc tcgctcctga acggtggaca gttttgtatt cttcgttcgt attcacgggt
     1081 aatctgctcc agaatatcct ccggcggtgt cagccactta ttcctgagca atacactggt
     1141 tgatggatga cagctggcta tttcagcaat aatttcacgt aatgtttccg ggctgaactt
     1201 cttcagctga ttgtagtgct cccgggtaaa accatgttca tcgcataaaa gttgccatgt
     1261 cagtgatata ctgtttgcca ttccttgctt catcattaat acctgttacc tgaatatcaa
     1321 cagaccggtg taccttttct gaatacctcc ccggacggag tggcaatccc cattcgcctc
     1381 agcgtggcat ctgcagcttt cgccacctcg ctgatatcca gcttttcagg tagcggcggg
     1441 gacgatatct gcgccagaca ataattccgg cgcaacaaca accactcccg ttgtgcttct
     1501 gtacattcaa catttcccat aacaaaaagc cagcggtcag aagctctgcg ccagcaacca
     1561 gcagcctcca gtttctgcgc aaccatgtct tccacaactt ccggggtact ctttttcctg
     1621 ctcaaaacat taactcactg aaataatata catatctgac ggtctcagcg aaccgtccat
     1681 gccgggctga aatcctcttc ctgaaacagc tccgtcagcc cgctaatctg tttcaatctg
     1741 agaacttcat cctggtccat acccagctcc gtaccaatgc gttcattcgt ccagcccagg
     1801 cgcgacaggt ccctgacaat atcagacatt gaggttatct gatgtttgcc cctggcccgg
     1861 ttatgtcgga tggtcgcagc aatacgtgag gcctgtccct ttctctccgg attaatacag
     1921 accaccggca gccagccctt cagacgtttt cccgtacctg cttccctgcc cagcacctgg
     1981 cgatgaaaac catccaccac caggtaatgg ttcttttcct cagacaccac caccggttgt
     2041 gtgaaaccat ctttctccag tgactgcttc agcagtttct tctcacccgg cgccatgaca
     2101 ttaggattgt aatcattggc caccacctca tctgctttta cccacagcac acagtccact
     2161 ggctcatgtg caaaaggact gacttcatga agcactctgc gcaggtgatt aagggcctgt
     2221 atggtctctt cttctggtcg ttcctggctg aagtactgac gaatcatgtt gccaattatc
     2281 tccggtgtca cagaatcccc cattccttcc tcctttcttt catacgctgc agataacgtt
     2341 catagtgccg gggtttgttc ggactgaagg agagggtccg gcaccagaaa tcattcttta
     2401 taagtgtctt acagatacgc cgccaggacg gaatatcccg gctccccagg tcattctcct
     2461 gttcatccgg gatgtcatcc gggaagcccc gcgtctgata ccagcgcagg tagacagcaa
     2521 ttttattacg gtaatgttct gccgttcttt ccggcatcac atccagcagg aacatcgcat
     2581 agctgcgcca ggtatgatgg gctggttttg atatacgttt acgcagggca aaataggcac
     2641 cgctttcatt ggcataaagc gccccgctgg cagcgcctga cacccgctca cacatcctgg
     2701 cccaggtttc cggctccaga atatggtaaa gccacagccc cttacgttgt tcaggaccaa
     2761 aaggttcaca gacacgcatg ttgcgtaacg gcacgtcggc acggtacatc aggtcataca
     2821 gaggattgta aatggcacaa gctctggcgt tatatatcca gatatcgcgg gctttccagt
     2881 catacaacgg atacatggtg taataaaacc cttcaggcga tgccgtggtc cagggcttat
     2941 catcggcata tctcagtttg cgctgagaca ccagccccat aaagcgattg agggattcat
     3001 ccgcacgaac accggtcagc actgccactc cacaccggtt accggcaaac caggaagaaa
     3061 atgccggaac aaattcttca aacgtcatgg cataccggaa aaagggaaaa tatgtcatat
     3121 ccgtaatggc ctcttccggt ggctggcgaa cccaggtcac tcctggctcc cagcatatcc
     3181 actccggctg aaactgcgag acgccgttta ccgtggtcag gggaagtgcc acccagtaaa
     3241 aggtttccgt cacatcatgg tacatttccc gcatcttctg aatatgttca atggtgcacc
     3301 gatactgggc ctcccagtca atgaacagaa cagagaaatg acgttttctc ctgcgggcca
     3361 cctctgccac aagatggaaa agcacggtgg aatcctttcc acctgaaaaa gacagacata
     3421 ccgacggaaa tgtttcaaaa atccactcaa tgcgggcgat tgcggcacgc aaaacatttt
     3481 ctccggtaag gattttttcg gttaaaagcg tatcactcat tttcctggct ttatgtcgaa
     3541 taattctgtt tatacattca ggcaaacttc attaataact caccgctttg gggataaata
     3601 ataacaacaa aaatcttcag atattaacct ggttgttgga tttgttatca catcctttat
     3661 ccacctgctt cttactggta acacatctgg aaaatccatg cattttcaac atatactatc
     3721 ctttcaactt gtaaataaga aaatatgatg cagatggtga acaattcaaa tatcattttg
     3781 caggaagtat tcactgtaat agaattaccc cctgataaag tttattcact aaggaattat
     3841 tcaacagaaa cattccctgt tagctttgag caatagcact tatttttata tgtatcagga
     3901 atacgtacgt tgatgtaacc aaaacaaaga agcctgtgtc catggggttc gtacaattgt
     3961 gtgtggcaca tgaccaaatc aggtttgaga cctcccgctg caatattttt tattcagatt
     4021 ttagcctgtc aggcctgaca gaaacgctcc caacttccct tctatatcac tgtctatagt
     4081 acgtaatctt agcaatggca ggccgcattt cttcagtata ctgtttttaa gttcatcacg
     4141 ttcagcctga acactggtca gatgataacc tccatccact tcaataaccc ctaatggagt
     4201 cttcccgacc ttatagtaaa caacaaagtc acaactggcc cggttacgca tgaataactg
     4261 ttcacggtgt gtaaaatccc cccgctccag caacaccaac tgattcagcg ctatttgtga
     4321 atgaaacatc atactccgat aactatcctg cgacaggata tcccgcaaca ggcaggccac
     4381 aatctgttct gacttaaagt gtgaatcatt tgaattcaat ctactattca gtcgttccag
     4441 tgatttgtca tactcactgt acagcaaatc aaatgctgat atgaccgggc tctcgaaaat
     4501 ctctccgtca tcagcgtagt atttgatgta acgaatcagc gccgcaatat gtccagcgtg
     4561 gcgttcaaat acatcattac cagtcaccag cgtaaattta tttcgggccc ttgaaacagc
     4621 gacattcacc agttcaggat tatcaacaaa cgcgatgttc cgactgtgct ggctgctgcg
     4681 cttcttatcc agcacagtgg aaaacacaat ttcatcgcac tcccgtccct gaaatttatg
     4741 caccgtcgat ttcacaaaat cagcaggcag cactttttca gccagattaa cctgtgcatt
     4801 atatggggca atgtatcccc tgctactttc ttcatcccag taatgacctt ccagagattc
     4861 aagctccctg agatttgaaa aattacgggt atggtttcct tttgccgtga tgaccagaga
     4921 taatgaggct tcaccggaat caaccgttaa cggtatcaga gcattatcat aaaactgctt
     4981 attacaaaat tgaatgattt taggatgaca acgataatgc tctttaagaa gggttactgg
     5041 caccatgttc ctgaacaaca tacaaaccga atcaagtaga ctatatttct cacaattata
     5101 gtattctgcc ggtggagatg ggctgttcgg gagtaataca ggaacatgtg gaagttgctt
     5161 acgatcccct acaacgatta cattacgggc acatcccagt cccagaatgc ccggaacaat
     5221 atcctgctgc gaggcttcat caataataac ataatcaaga agggccccct tgccgataga
     5281 gttaataatc gaatgcgtac tgctaccaat aatcggaaaa cgctttataa aacggtcaaa
     5341 ttcatccctg tatgtctctg ccgagaatga acttcttgtt gaaacatttc ttcgcagatg
     5401 ttgctttaaa taaagcatag accatgatgt caggcgcccc agtaaaattt taaaatcaga
     5461 ttttttaaat acttcctcat attcattcag agaaatttgt ttttcccgga tatttttttc
     5521 ataataagaa agctgaagag aagtaaatag cgccagcctt ctctccggaa tcatcaatgg
     5581 tttgactttc agtatcatga agtttaacaa caaatcaatt ctgtttctga atccaatccg
     5641 cctgtcagaa aggtaatgaa tataagccat caggtcggtt gttttttgtg aggaaaatcg
     5701 gtatttgtga gaaaaaagct cctccggtcg aagattctga ctctgccatt gctgcaggta
     5761 tttatactca atattcagct cattaatttc tgcttttaac gaagcgacct ggttaatggc
     5821 attcaggtgt tttttcactt gttgaagtac atcatcaatc gcatttgcag acggtgagtc
     5881 aggtaagact tcctcagaac gcgaaatcga cgttgaaaaa aactgttgcc tgttctccgt
     5941 actccccagt cgtgccatca catatcccag ttgctgtttg tccatctttt cataaacatt
     6001 actgacagct gagttattat tggacaaaat tgccactgtt ttattctgaa ttaatatatt
     6061 agcaacaata ttgagtatag tctgtgtttt acctgttcca ggtggtcctt caataactga
     6121 aatctgtgag gaaaatgccc gtttgacagc cagcaactga ctttcattaa gaccaaacgg
     6181 aaaaatcaaa ccccatggcg aatcacgttt tttactctgt cctgaacagt atgcatgcaa
     6241 ggcggtatcc ggatgaggaa ctattttctt tatctgccta aggatattct catcgatatt
     6301 tctgttatta ccacgggcat aaaaaatacg ttcattagcc accctacaca agtaattaaa
     6361 cacggcatgc tctgtaaatg ctgaacactg ttgaaagtca aggccggtcg ttttcatgat
     6421 gtagggtcgt tcaccctcgc ggtaataaac agcagcatat ttatcaccat atataataac
     6481 cagttcagcc cgggatgtta cagcgttccc tttcctttcg aggaaaacat ttcgcaaatt
     6541 tacagttggc gttatctgac atacagaaag cggacgagta tattttctcc cgctgcgaaa
     6601 atgacaggtc agcataacct ctctgtcgtc atttccagcc cacagcgtcc agtcggtaat
     6661 tttatgagtc ttattttcac cgtcaacaca aatcgtgacc atattctcac tcagaaatta
     6721 tcatttaact tacggaaaat tgttaaggca ctcccgatat tagctccgga ggattttcca
     6781 caactccggg actgttaaca tatttctgcc tgccatatat acattgaatt tagtcataac
     6841 agtcaatacc agaattacag gaatgcgatg ccgatacgct ggttattgaa tactggtagc
     6901 ccctgagcac taattcatca cgatactctg tatttactgt tttcactgct ttttacaacc
     6961 attcccccag gtaaaatgct gctcctccgg tgatggctgt gtcagaaact gccatcccgg
     7021 aatagccacg ctgaccgcca gcaatgccgc acagacacca gccacaaacg gtttcagaaa
     7081 cgtgggcggg gatttctgaa gggagggtga ctgctccgcc tgcactttcc ccttgcgatt
     7141 atcaaggctc agtgcatcca gacgcaccgc gccatttgcc gcaagcagcc cttcccggtt
     7201 atccacgctt cccggcagac tcagcgaaaa atcccctgtt cccgtctgaa tcagactgcc
     7261 tttacgattt gacaggcttt gtgccgatat ctgcagtgtg tcggcattta tcgtaccgcc
     7321 attactgctg aactgcccgg cagtccgggc ggaaagcgta ccggcactca gttgcgcatt
     7381 ctgtgtagac agatttccac tggttgcatt cagctcagta tgttgtccct gtatccggct
     7441 gttacctaaa tccagcccct gctcaatgtc ccgtaaatcc agcagagaac cttcgtgtgc
     7501 cgggaatgag gaatacaact gaatatagtc atcactgtcc ggtgtcagac gcacatgtcg
     7561 cacaacaccg ggaataatga ccagccggag gataccgctt tttaaatcct gcgaaggtgc
     7621 cagaacacgg gtggtgacat aaccatgatc gaccagacgg ttctgcaatg tactcatcag
     7681 cagattaatt cctttcgccc ccaggcaatg cccgaccgcc ccgttggcga ttttttgtaa
     7741 aggcagccag tgtggtaacg catcagcccc cttcagttcc gtctgtttaa tctgaaaaca
     7801 gggcgtttca acaggaaaat taatcttatg ggcaaaagag ccaggtgcag aaaggcgaac
     7861 atcaggggct gaaggcgtca gttgttgctc aagggcttcc tgacgctgct gctgaatgat
     7921 aaatgcttca tctccgctac gccggacatc cgctgcctga agagataaag gcgataataa
     7981 agcagacatc agtgtcagtg acagcaagga cggacagcgc cggaataagc tttgttcctc
     8041 agtcctctcg ttcctgcaac ataattttcc agtcaaacga aaaacctttt attcatgtca
     8101 taccaacaat caccagcctc ctgacgatac ctcccaccat gttttgcgat ggcacgctac
     8161 cgcccttccg gtatttaccc gcatcaaccg gtaccagaat gctgagtaac aaaccagcca
     8221 gaattgactt aattttgatc attatagaat aatgatctga actacagcaa catgacagat
     8281 ttatcgatcg atataacaca aatgaacagt tctttattct gccatatatc gcaaaaacag
     8341 cacattacaa aatttataac cataattact ggaaaagtgc agaaaattat tccactgaat
     8401 gcttttaata cagttggtta gcttaaaaaa tagcaatttt tgatttcagt cattgaaagt
     8461 tgcaatgttt gacgattcaa cagtatttaa gtgagtttca ggatatggga aggacatgaa
     8521 aggaggagcg caataaaata cacaggaaga aggggaaaat gtttcgtaaa aacagaaaat
     8581 taacagcgca catcaggaaa cgcagtctcc tgattcagtg ctaccacatg aaaatttagt
     8641 taactgttac cgtactaaaa tttatgtcag aagatgttcc attaccgaaa gtcaatcagc
     8701 gatacaaaga cgatcatggt gcgcttgtca ccctgaccag tgttgaggaa acccgggtgg
     8761 tgtttatgcg ggacggctat ccgcatccct gcatgcaacc gatgtacaac tttctgggaa
     8821 aattcaaacc cgaaccccgg gaagaaacag aataaaacga aatccctgcc ggcaatcaca
     8881 ctacctgata gcagcataat ccacttatta attctcatcc ccggagacaa gcccagaaaa
     8941 ccactccggg tgttcactgt atgccgtgtt cagcgcatcc ctggaaacgt ttatataaaa
     9001 gctgtcctgc aatttgcggg taacaacttc tgccaggcga ttccggttat ccaccagtcc
     9061 ggcataacct ctctctgcca gccactcatt cagttcatgt tccagagaca gattgataat
     9121 atcataatcg gaccttgcca ttcccgtttc cttagcacat gttttcaggc acggggataa
     9181 agtataaaga atgtcatact gctgctgcca gagcaatatt ccccctgatg gttgtatcag
     9241 agatggtatg ccggtacttt gcgggttgtt cccgtcaggt tatcgtgcac ctccacggtc
     9301 gtggtcacca ccggcattcc ggcctttctc agcctcagaa catcagccgc aatacgctga
     9361 ctgccaaacc agaacaggcc gtccagtgca gtcaccagca aacccgcctc cagtgcatac
     9421 ttcagccgtt cacggggcgc tttcacttcc cgggcaacct gctgatatgg caatgatgtg
     9481 ttttcattcc caaccacgcg acgaatacgg tgagacagat gataccggta tgtatccggc
     9541 acaccagaaa ggctggcctt caggctgtac acgcagccaa accgtttatc gttaaacacc
     9601 acatttttct ggctgatgcc ccattcttca cgcagcgcgg caatcaattg cggtgtacgg
     9661 gtaagcagca agcgaaaagg cagttcaaaa ctggtgacgt aatccacatt cagcagtgca
     9721 atacgcaacc gttcttctgg cccggcctct gtctgccggc actcctccag aacatcctgc
     9781 cactgccggc gaagacgggg ggattcgttc cgttctgtaa aacagtattt atccgccaga
     9841 tagtcaattc gtgtacgcat actgaaagga attccgtata aagattcagc agacaaaact
     9901 ttatcagcca gtaaaaatta acggaagcat tgatattcct cccgacaatc accggatgat
     9961 tgttgcaata acctgcgaca taaaggactg aacagccgtt tttaacgcca cgtattgccc
    10021 tgatgaacca ggccgggcaa cccgaaaaca tctttccacc cggcattgtc cgccagcact
    10081 tatcaccgac caggtctgtt gcagtaaatc caccagttgc gcacattctt catcagccat
    10141 gacgccgact cgcggatggc aaatactcgt ctggctgagc agcaacagac atcgtatctg
    10201 tgctccttca tgttgttccc gcaaccagcg taataccacc ggcgctgatg ggctgaccgt
    10261 gtgattatgc tccgtaatac gttcgtgtac ccgtcggtga aaggaactac aaaatgtctg
    10321 aatctgttga ccatagattc cttctgccga atagagcgtg aagtgaaatg ccaacagatt
    10381 tggccagtgg tccaccgcct gctgaacaaa acgccggatt tcccccggct ctgaaattaa
    10441 ggcttcggtt atttgcacga aatgttcctt ttcaacccct gttgaatttt gtcaagccgg
    10501 tgcagacaca gtaaagcgcg catcaacacc tgaaaggtac gatacaaaca caagttacaa
    10561 acaataagaa cggaaggaca attaatcagt gaggaataat aactaaatta accaaaagaa
    10621 tgttactgac aatactacca atacttaaat agcaatatat taattaatgt aagaacagat
    10681 gcccggtaag acatatacac aatacatcca caaacacact cttttcatta aatcaatcac
    10741 ctgattaaac catgaaagtg tgatgtaagt caaaattact gatactatca attgataatg
    10801 attatctcta tcattatcgt gaaattcgag aatgaaagga gaagtagtca ttataagcct
    10861 tttataaatc gtcatcatca tagtaaagta gaacactttt tatatgagtt actatatgaa
    10921 aatatactgg acaagaaaac gtattcctga actttcagca cttccacctt cattaagaaa
    10981 gaaaaatttt actgacgcat ataatgcagc atcttctcat atcgaatact ggataggtgc
    11041 tggcgtctca tttatctcga tgatgatttt atccagagta tatgactttc ttttacccgc
    11101 ccaagatact ttcccgggtg atatcatcag aagtttatgt gttgtgtgtc catccatatt
    11161 aatctggttt cagttttcgg tatatgtcat gaggaaatat tacagacata ttctggtgcg
    11221 aggaaaagaa accgaaacca tttcagaaag gttgatacgt gaggctgata cacgagaata
    11281 tgaactatgg cgtccggtcc gtcgcttttt ctccattgtt ttcctcattg ttctattagg
    11341 ttgcattcat tcactgataa caactataaa ataaaagtta tggctgttct gatacaggaa
    11401 aataaacgtt cattctatgg ttacctcttc ctgttatata gaagaatttt aaacgacaag
    11461 taagggaata taaatgaaaa taacaaaaaa taaaaatgca actataaaaa acagtcatca
    11521 gtcggctgct tttcaatgtt taccttatc
//
LOCUS       DADMGC010000124        11545 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.124, whole genome shotgun sequence.
ACCESSION   DADMGC010000124 DADMGC010000000
VERSION     DADMGC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.124"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            9..461
                     /locus_tag="JNZ73_003308"
     CDS             9..461
                     /locus_tag="JNZ73_003308"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8733483.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYVFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            707..1751
                     /locus_tag="JNZ73_003309"
                     /pseudo
     CDS             707..1751
                     /locus_tag="JNZ73_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1867..2427
                     /locus_tag="JNZ73_003310"
                     /pseudo
     CDS             1867..2427
                     /locus_tag="JNZ73_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570718.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(2484..3941)
                     /gene="pepD"
                     /locus_tag="JNZ73_003311"
     CDS             complement(2484..3941)
                     /gene="pepD"
                     /locus_tag="JNZ73_003311"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY8733484.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTITEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4203..4661
                     /gene="gpt"
                     /locus_tag="JNZ73_003312"
     CDS             4203..4661
                     /gene="gpt"
                     /locus_tag="JNZ73_003312"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY8733485.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4753..5997
                     /gene="frsA"
                     /locus_tag="JNZ73_003313"
     CDS             4753..5997
                     /gene="frsA"
                     /locus_tag="JNZ73_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY8733486.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPQGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            6055..6456
                     /locus_tag="JNZ73_003314"
     CDS             6055..6456
                     /locus_tag="JNZ73_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY8733487.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6495..7550)
                     /gene="phoE"
                     /locus_tag="JNZ73_003315"
     CDS             complement(6495..7550)
                     /gene="phoE"
                     /locus_tag="JNZ73_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY8733488.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            7838..8941
                     /gene="proB"
                     /locus_tag="JNZ73_003316"
     CDS             7838..8941
                     /gene="proB"
                     /locus_tag="JNZ73_003316"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY8733489.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8953..10206
                     /gene="proA"
                     /locus_tag="JNZ73_003317"
     CDS             8953..10206
                     /gene="proA"
                     /locus_tag="JNZ73_003317"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY8733490.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            10321..10396
                     /locus_tag="JNZ73_003318"
     tRNA            10321..10396
                     /locus_tag="JNZ73_003318"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10354..10356,aa:Thr,seq:cgt)
     gene            complement(10411..11439)
                     /locus_tag="JNZ73_003319"
                     /pseudo
     CDS             complement(10411..11439)
                     /locus_tag="JNZ73_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2767 a         2861 c         2972 g         2945 t
ORIGIN      
        1 tttatattat gaataacata caaataagag attatcagcc tggcgatttt cagcaactat
       61 gcgctatttt cattagagcg gttacgatga ccgtcagtca gcattattca ccacaacaaa
      121 ttgccgcctg ggcgcagatt gacgaatctc gctggaagga gaaactcgcg aaatcacaag
      181 tgcgggttgc agtcattaat gcaaaactgg tcggctttat tacctgcgtt gaacactata
      241 tcgatatgtt atttgttgac cctgaataca cccgccgtgg ggttgccagc gctttgttaa
      301 aaccttttat taagtctgaa tccgaactta cggtggacgc aagcataacc gcaaaaccct
      361 tttttgaacg ttatgttttt cagacagtta agcagcagcg cgttgaatgc cggggagcgt
      421 ggtttattaa tttttatatg cgttataaac cgcaacatta aatccagctt gcaatgaaaa
      481 taacgcccgc ctagtatgtg cgcgttttct tatttcacaa actgcaagga ggtaaatcat
      541 gacaaatcct ttatcaatga ctctttgcag acctttccag gattaattct ttttttcttg
      601 ccctggattc gtctgccatt tcctgatttt ttaattgtta ctctcaataa ttcaagtcgc
      661 agcacatgca acttgaagta tgacgagtct atttacggaa tggtatatgg gcaattatat
      721 tcgtccctta tccgatgcgg tatttaccat cgcatctgat gacctgtgga tcgagagttt
      781 agcgatccaa caattacaca ccacggcaaa tttacccaac atgcagcgcg tagtggggat
      841 gccagattta caccccggac gcggctaccc gattggcgca gcgttcttct ccgcaggccg
      901 tttttaccca gcactggtcg gcaatgatat cggctgtggc atggcgctat ggcaaacaga
      961 tattctcgct cgcaaataca acgccgataa gtttgaaaag cgattatcag ctttggatga
     1021 cgttgctgaa gaaagctggc tggaggaaaa cctgccgtca gcatttgcac agcatccgtg
     1081 gcgcagctcg cttggctcta tcggtggtgg caatcatttc gctgaactgc aacaagttga
     1141 tcaaatcatc aacgctgaac tgtttgcact ggcaggtctg gatgcgcagc atctgcaact
     1201 gctggttcat agcggctcga gggagctagg gcagtctatt ttacagcggc atattgcctc
     1261 gttttcgcat catggtttgc ctgaaggcag tgacgacgcg ctggcgtttg cgcgttttaa
     1321 tcgccacctg atcgctttgc gcataatgca acaggttaag gccaccggaa gtccggttct
     1381 ggatgtggcg cataactttg ttagcgcgtg tcgaatcggt gatcaacaag gggtcttaca
     1441 tcgtaaaggt gccacgcctg atgactgtgg tctggtggtt attcccggtt cacgcggtga
     1501 ttactccagg ctggttcagg cccgtcagga gtgaggaaac attgcattcg ctggcgcatg
     1561 gggctgggcg taaatggggg cgcaccgagt gtaaagggcg tctggcagcg aaatacacag
     1621 cgacgcaact ctcgcgtact gaacttggca gtcgggtaat ttgtcgcgat aaacaactca
     1681 tcttcgaaga agcgccacaa gcctataaat cggctgaaag tgtggtgcaa tgtctgggca
     1741 ggccggatta attattcctg tcgcgcggct gcgtccggtg ctaacgctca aaaacagtgg
     1801 agggaaaaaa ggatgatctt gctataactc tcctcagcgc aggggccgga agaatgttgt
     1861 ctcgcagtga aaaaggcgct ggacaggcta attaaagaag ctgcccgaca agacgtcgcg
     1921 gtaacggtgc tggaaacaga aacaggccgc tactctgaca cgctgcgttc ggcgctgatt
     1981 tctctggatg gcgacaacgc atgggcgtta agcgaaagct ggtgtggcac tattcagtgg
     2041 atttgcccga gcccgtatcg cccacatcac gggcgtaaaa actggtttct gggcattggg
     2101 cgttttaccg ctgatgagca ggagcaatcc gatgcgatcc ggtatgagac gctgcgttcg
     2161 tcggggccgg gcggtcaaca tgtcaataaa accgactcgg cggtacgcgc cacgcatttg
     2221 gcatccggta ttagcgtgaa ggttcagtca gagcgcagcc agcatgctaa caaacggctg
     2281 gcgcgattgc tgattgcctg gaagctggat cagcagcaat aggaaaatag cgcggtgctg
     2341 aaatcacagc ggcgaatgtt ccatcaccag attgaacgtg gcaacccgcg acgaacgttt
     2401 acagggatgg cttttatcga aggataatga aggatgaaac tgccggaagg cgattaaacg
     2461 ccatccggca gcaaatcaaa taattacttc gccggaattt ctttcagcag ttcagtcagc
     2521 agtgtccagt aatgacctac gctttcgatg tgaacttgct catccggaga gtgtggaccg
     2581 gtgatagttg gcccgataga aaccatgtcc atttccggat acggtttttt gaacagacca
     2641 cattccaggc ccgcgtggat aatctggatg ttcggcgtct tgttgaacag gcgctgatag
     2701 gtttcacgta ccagatgcat caccggagaa ttagcgtccg gctgccagcc aggatatgcg
     2761 cctttcgctt cggttttcgc gccagccagt ttacccagcg aatccagcat gctcaccacg
     2821 tagtctttac cgctgtcgat cagtgaacgg atcaggcagt gaatttctac gttattgtca
     2881 gtcatggtca ccacaccgac gttcagggag gtttcgacca caccttttgc cacgtcggaa
     2941 ttgcggatca caccgttcgg ggtggcgttc agcagacgaa taaaggtatc gcgagatttc
     3001 gcaatcaggg cagctttatc gttcgctaca gagtccagca acaaggccag atttttctct
     3061 ttctctgcca gctcgttttt caggatctcc tgataggtat tcaccagaga tttcagtgcg
     3121 tcgactttat cagctgtgac agcaatggtc gcaaaggctt cacgcgggat ggcgttacgc
     3181 agtgtgccgc cgttgaaatc gataaggcgc aggtccagtt cttctgcatg acccgccagg
     3241 aagcgcacca gcagtttgtt ggcattaccc aggccaacgt ggatttcacc gccggagtga
     3301 ccgcctttca gaccttttaa ggttaacttg aaggtttcaa aaccagctgg aaccgcttca
     3361 cgatctaaat gcaggttgga ggtgaagtcg ataccccccg cacaacccat gtagatttca
     3421 ccttcttctt cggagtcggt gttaatcaga atatcagcct gcaaccagtt gctctgtaag
     3481 ccgaacgcac cgtccatacc ggcttcttcg gttatggtca gcagcacttc cagcgggccg
     3541 tgaaccacgt tttcgtcagc cagaaccgcc agtgcagagg ccataccaat gccgttatcc
     3601 gcacccagcg tggtgccgcg cgctttaacc cattcgccat caatataagg ctggatagga
     3661 tctttcgtga agtcatgcac ggtgtcgtta tttttctgcg gcaccatatc gaggtgggcc
     3721 tgtaagacga ccggtttacg attttccata cctgcggtag caggtttacg aatcaggata
     3781 ttacctacct gatcgcgttc gacatggaaa cctttctctt ttgcccaacc aacaatgtat
     3841 tcagcgagtt gctcttcatg ataggacggg tgaggaatag aacagatttt ggcaaaaata
     3901 tcccacagcg gctgtggaga taattgagac agttcagaca cgttaagtct ccttgtcgat
     3961 cacccgcaaa acagtattgc aggtcacagg gttagcagaa aatgttgtca acacaagaca
     4021 ggcttgcgag atatgtattg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
     4081 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatatc tataatctcg
     4141 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
     4201 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
     4261 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
     4321 tggtaccggg ggcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta
     4381 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg
     4441 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gctgttgcga
     4501 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc
     4561 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggatcgaa cagccgtggg
     4621 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcat
     4681 tgccgggcgt tgttcttttt aacttcaggc aggttacaat agtttccagt aagtattctg
     4741 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa
     4801 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag
     4861 tcggctcttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg
     4921 atctggcgcg gtattgaccc acgtgaaatc ctcgacgtcc aggcacgtat tgtgatgagc
     4981 gatgctgaac gtaccgacga tgatttatac gataccgtga ttggctaccg tggtggcaac
     5041 tggatttatg agtgggccac ccaggcgatg gtgtggcaac aaaaagcctg tacggaagaa
     5101 gatccgcaac tcagtggtcg acactggctg catgcggcta cgttgtacaa cattgccgcc
     5161 tatcctcatc tgaaaggaga tgacctggcc gagcaagcgc aggctttatc aaaccgcgcc
     5221 tatgaagagg ccgcccagcg tctgccgggc acgatgcggc agatggagtt taccgtaccc
     5281 ggcggtgcgc ccatcaccgg ctttttgcat atgccgcaag gcgatggccc gttcccgaca
     5341 gtattaatgt gtggtggtct ggatgcgatg cagacggact attacagcct gtatgaacgt
     5401 tattttgcgc cgcgcggcat tgcgatgctg actattgata tgccgtcggt gggcttttct
     5461 tcaaaatgga agctcaccca ggactccagc ctgttgcatc agcacgtctt aaaggcgctg
     5521 cctaacgtac cgtgggtgga tcacactcgc gtcgcggcct ttggtttccg tttcggcgct
     5581 aacgttgccg tgcgtctggc ataccttgaa tcgccgcgtc tgaaagcggt tgcctgtctt
     5641 ggtccggtag ttcataccct gttgagtgat tttaagtgcc agcaacaggt gccggaaatg
     5701 tatcttgacg ttctggcgag tcgtttgggg atgcatgatg cttccgacga agcgttacgc
     5761 gtggagctga atcgctattc attaaaagtg caaggattgc tggggcgtcg ctgcccaacg
     5821 ccaatgttat caggctactg gaagaacgat ccgttcagcc cggaagagga ctcacgctta
     5881 atcacctcat catctgctga cggtaaatta ttagagatcc catttaaccc ggtgtatcgg
     5941 aattttgaca aaggtcttca ggaaatcacc ggctggatcg aaaaacgctt gtgttaaaaa
     6001 tttgctaaat tttgccaatt tggtaaaaca gttgcatcac aacaggagat agcaatgacg
     6061 ttaccgagtg gacacccgaa gagcagattg atcaaaaaat ttaccgcact aggcccgtat
     6121 attcgtgaag gtaagtgcga agataatcga ttctttttcg attgtctggc tgtatgcgtc
     6181 aacgtgaaac cggcaccgga agtgcgtgaa ttctggggct ggtggatgga gctggaagcg
     6241 caggaatccc gttttaccta cagctaccag tttggtctgt tcgataaagc aggcgactgg
     6301 aagagtgttc cggtaaaaga tactgaagtg gttgaacgac tggagcacac cctgcgtgag
     6361 tttcacgaga agctgcgtga actgctgacg acgctgaatc tgaagctgga accggcggat
     6421 gattttcgtg atgaaccggt gaagttaacg gcgtgagtga aatgtgccgg atgcatcaca
     6481 tccggcaata ttcattaaaa ctgatacgtc atcccaaccg cgacagtatc atcattatta
     6541 atattcagct tattatcgct atccagtttg ttgattttat aatcaacaaa cgctgacata
     6601 tttttgttga aataatacgt agcaccgacg tcgatataat tgaccagatc ttcatcaccg
     6661 atgccctcaa tatctttccc tttcgataag acataaccca gcgatggacg cagaccaaag
     6721 tcaaactggt attgagcgac cgcttcaaag ttctgtgtct tattggcaaa gccgccagtt
     6781 attggcgtca ttttgcgtgt ttcagaatag aaagttgcca gataaatatt attggcatcg
     6841 tatttcagac ctgttgccca tgcttctgca cgcttgcctg tgccacggct ttgcaggttc
     6901 tgctcgttgg tgcgatctga gttggtatag gccccactaa tggcgaaatc gctgccgcca
     6961 aagtcatatg tcaatgacgt gccgaagcca tcgccgtttt gctttttaac gtcgcggttt
     7021 tcgtttttcc cttgatattg cagggttaag ttcaggccat cgataacgcc gaagaagtcg
     7081 gtgttccgat acgtcgccag accgctggcg cgtttggtca taaagttgtc ggtctgcgcc
     7141 gaggaatcgc caccaaattc cgggaacata tcggtccagg cttccacgtc atacaacgcg
     7201 cccaggttac gaccataatc gaaagaaccc aaatctttat atttcaaccc ggcaaaagcg
     7261 agacgcgttt tttgctgtgc agtatcactc tccgctttat ttccggcaaa ctccgcttcc
     7321 caccgaccat aaccagtcag ttgatcgtta atttgagttt cacctttaaa accaaaacgg
     7381 atataactct ggtcgccatc tttactggcg ttatcactca tataatgcat ggctttaact
     7441 ttgccataga catccagttt attaccgtct ttattatata tttctgcagc ctgtacagat
     7501 gcagatgcca caatgcccat caccactaat gccagagtgc tctttttcat tttcattcct
     7561 gattttaatt aacgcgcgaa tattcagcgg gagagccccg ttgaaaacag gaaagttttt
     7621 aacctgagat tgttaaagat atattacaga ttaatgatat tctaaaaatg tggtaattta
     7681 ttaaatctgt aataaaagcg taaacaactg ccgctacgct tgctgatccc gcgcaacaaa
     7741 acgccatgct ttgctcgcag atggttggca accgacgaca gtcctgctaa aacgttcgtt
     7801 tgatatcatt tttcctaaaa ttgaatggca gagaatcatg agtgacagcc agacgctggt
     7861 ggtaaaactc ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc gtgcccatat
     7921 cgttgaactt gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga ttgttattgt
     7981 gacgtcgggc gcgatcgccg ccggacgtga gcacctgggt tacccggaac tgccagcgac
     8041 tatcgcctcg aaacaactgc tggcggcggt agggcagagt cgactgattc aactgtggga
     8101 acagctgttt tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc gtgctgatat
     8161 ggaagaccgt gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc tcgatcacaa
     8221 tatcgttccg gtaatcaatg agaacgatgc tgtcgctacg gcagagatta aggtcggcga
     8281 taacgataac ctttctgcgc tggcggcgat tctggcgggt gccgataaac tgttgctgct
     8341 gaccgatcaa aaaggtttgt ataccgctga tccgcgcagc aatccgcagg cagaactgat
     8401 taaagatgtt tacggcattg atgacgcact gcgcgcgatt gccggtgaca gcgtttcagg
     8461 cctcggaact ggcggcatga gtactaaatt gcaggccgct gacgtggctt gccgtgcggg
     8521 tatcgacacc attattgccg cgggcagcaa gccgggcgtt attggtgatg tgatggaagg
     8581 catttccgtc ggtacgctgt tccatgccca ggcgactccg cttgaaaacc gtaaacgctg
     8641 gattttcggt gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa ctgccgccat
     8701 tctggaacgc ggcagctccc tgttgccgaa aggcattaaa agcgtgactg gcaatttctc
     8761 gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc acggtgtcag
     8821 tcgttacaac agcgatgcat tacgccgtat tgccggacac cactcgcaag aaattgatgc
     8881 aatactggga tatgaatacg gcccggttgc cgttcaccgt gatgacatga ttacccgtta
     8941 aggagctggc cgatgctgga acaaatgggc attgccgcga agcaagcctc gtataaatta
     9001 gcgcaactct ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga tgaactggaa
     9061 gcacaaagcg aaatcatcct caacgctaac gcccaggatg ttgctgacgc gcgagccaat
     9121 ggccttagcg aagcgatgct tgaccgtctg gcactgacgc ccgcacggct gaaaggcatt
     9181 gccgacgatg tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt aatcgatggc
     9241 ggcgtactgg acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg ggttattggc
     9301 gtgatttatg aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg cctgaaaacc
     9361 ggtaatgcgg tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc ggcaacggtg
     9421 gcggtgattc aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt gcaggcgatt
     9481 gataatcctg accgtgcgct ggtcagtgaa atgctgcgta tggataaata catcgacatg
     9541 ctgatcccgc gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc gacaatcccg
     9601 gtgatcacag gtggtatagg cgtatgccat atttacgttg atgaaagcgc agagatcgct
     9661 gaagcattaa aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg taatacggtt
     9721 gaaacgttgc tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt aagcaaacaa
     9781 atggcggaaa gcggcgtgac attacacgca gatgcagctg cgctgacaca gttgcaggca
     9841 ggccctgcga aggtggtggc ggttaaagcc gaagagtatg acgatgagtt tctgtcatta
     9901 gatttgaacg tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat tcgtgaacac
     9961 ggcacacaac actccgatgc gatcctgacc cgcgatatgc gcaacgccca gcgttttgtt
    10021 aacgaagtgg attcgtccgc tgtttacgtt aacgcctcta cgcgttttac cgacggcggc
    10081 cagtttggtc tgggtgcgga agtggcggta agcacacaaa aactccacgc gcgtggccca
    10141 atggggctgg aagcactgac cacttacaag tggatcggca ttggtgatta caccattcgt
    10201 gcgtaaataa aaccgggtga tgcaaaagta gccatttgat tcacaaggcc attgacgcat
    10261 cgcccggtta gttttaacct tgtccaccgt gattcacgtt cgtgaacatg tcctttcagg
    10321 gccgatatag ctcagttggt agagcagcgc attcgtaatg cgaaggtcgt aggttcgact
    10381 cctattatcg gcaccattta aatcaataag ttacctcgca tttaagtaaa ccacgttctc
    10441 ctcttgtgcc gtatttgtgc cattgcgact tataatcgca tcgattttgc ttgcgtgctc
    10501 ggtgagatgc ccggctgaaa ggtgggcgta tctttgaacc atttcgagag tttcccatcc
    10561 tcccatctct ttaagtgcaa gaagagagac accggactga accagccagc ttgcccaggt
    10621 atgcctcaga tcatggaagc ggaagttgct aatgcctgcc cgctttaacg ctcccttcca
    10681 tgccttgttg ctgtcggttc tcatcttcct taccgctgct gtttttgttc cgtcgcttcg
    10741 gtaggcaggt ttggtgtgga caaataccca tctcttatgg agcccctgct gttttcttaa
    10801 tatctggcat gcggtttcgt taagaggaac tccgatcgca ttgccagctt ttgtttcatc
    10861 agggtgcatc catgccattt tcttatccag atcgacctgt gaccactcaa ggtctgtaac
    10921 gttggaacgg cgaaggcctg tcgtgattgc aaacatgacc acagggaaga agtgaggagc
    10981 aatttctgca aacaggcgct tcgattcctc ctctgtaagc catctgatgc gtccattctt
    11041 aacgcgtggt gttgatattt tgggtgccct gtcaagccat ccccattcaa cagccatatt
    11101 gagaatggcg cgaagtattg ccagatgcct cgtcttcgtt cctttgcttg caagctttgg
    11161 tttatactcc ggcaccggat tgccaagtcg caaacacctg tcccggctca tctcccagtt
    11221 caggcgatgg cggcggtttt ccatcccgtc taccgcctcc attatttttt ctgttgttat
    11281 gtcagagaga atggcttctc tgaagtgcaa catccagaac gatataatgc tcttgtcatc
    11341 atcaatggac ttcttatccg atttctcacg cagccaccgt atgcaggctt ccttgaatag
    11401 ctttttcggt gattccccga gattttttac tctccacgct ttgaagtggt caacaaaaac
    11461 tggccaccga gttagagttt ttccagtatc gattttccga ttcgtttggg ggtaacccac
    11521 cgttatattc gtgcggtctt agtgc
//
LOCUS       DADMGC010000125        11200 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.125, whole genome shotgun sequence.
ACCESSION   DADMGC010000125 DADMGC010000000
VERSION     DADMGC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.125"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            34..393
                     /locus_tag="JNZ73_003320"
     CDS             34..393
                     /locus_tag="JNZ73_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HAY8733491.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(513..692)
                     /locus_tag="JNZ73_003321"
     CDS             complement(513..692)
                     /locus_tag="JNZ73_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HAY8733492.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            766..2127
                     /gene="pabB"
                     /locus_tag="JNZ73_003322"
     CDS             766..2127
                     /gene="pabB"
                     /locus_tag="JNZ73_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HAY8733493.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQKVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2131..2709
                     /locus_tag="JNZ73_003323"
     CDS             2131..2709
                     /locus_tag="JNZ73_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAY8733494.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            2893..4257
                     /gene="sdaA"
                     /locus_tag="JNZ73_003324"
     CDS             2893..4257
                     /gene="sdaA"
                     /locus_tag="JNZ73_003324"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY8733495.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDNLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4388..5986
                     /locus_tag="JNZ73_003325"
     CDS             4388..5986
                     /locus_tag="JNZ73_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY8733496.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(5990..7540)
                     /gene="yoaE"
                     /locus_tag="JNZ73_003326"
     CDS             complement(5990..7540)
                     /gene="yoaE"
                     /locus_tag="JNZ73_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HAY8733497.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENCDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            8003..8974
                     /gene="manX"
                     /locus_tag="JNZ73_003327"
     CDS             8003..8974
                     /gene="manX"
                     /locus_tag="JNZ73_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAY8733498.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFIPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9037..9837
                     /locus_tag="JNZ73_003328"
     CDS             9037..9837
                     /locus_tag="JNZ73_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY8733499.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9850..10701
                     /locus_tag="JNZ73_003329"
     CDS             9850..10701
                     /locus_tag="JNZ73_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAY8733500.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10756..>11200
                     /locus_tag="JNZ73_003330"
     CDS             10756..>11200
                     /locus_tag="JNZ73_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HAY8733501.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
BASE COUNT         2750 a         2828 c         2827 g         2795 t
ORIGIN      
        1 cgtgccatat cgttataatc cctttgatat tagatgcaaa ttaaggtcat atatagcctt
       61 atcgataaca tggttaattt taaggataag aatatgcctg ctgtaataga taaagccctg
      121 gatttcattg gtgccatgga tgtatcagcg ccaacaccaa gttcgatgaa tgaaagcacg
      181 gcgaagggaa tctttaaata tttaaaagaa ctgggagtac ccgcgagtgc cgctgatatt
      241 acggcgcgag ctgaccagga aggctggaac cctgggttca cggaaaaaat ggttggatgg
      301 gcaaaaaaaa tggagacagg tgaacgctct gtgattaaaa atcctgaata cttttcaaca
      361 tatatgcagg aagaactaaa agcactggtc tgagttaaat ttatatcagc ataaatgggt
      421 caggacgctt ttaatcacat ataaaaagcg tcctttttct cacaatcaaa tgatcatcgg
      481 cttctataac gaaatcgata cggattagtg tgttactcgt cttcatcctc aaaacgcgcc
      541 acgattagct cgccggagtg gttggcacgc agctcttctg ccaccagcgc gattgcctgg
      601 ccgctactca tcccttgtgc catcagttcc tggatccgct cgacagcttt ttgctgctgt
      661 tcatgggtga gtgaaggtaa acctgcaaac attgttaact cctgctaaat tgttggcgct
      721 aattatttca tgctacccgg cacatagcca gtagagtcag gactgatgaa gacgttatct
      781 cccgctgtga ttactttacc ctggcgtcag gacgccgctg aattttattt ctcccgctta
      841 agccacctgc cgtgggcgat gcttttacac tccggctatg ccgatcatcc gtatagccgc
      901 tttgatattg tggtcgccga tccgatttgc actttaacca ctttaggtaa agaaaccgtt
      961 gttagtgaaa gcgaaaaacg cacaacgacc actgatgacc cgctacaggt gctccagaag
     1021 gtgctggatc gcgcagacat tcgcccaacg cataacgaag atttgccatt tcagggcggc
     1081 gcactggggt tgtttggcta cgatctgggc cgccgttttg agtcactgcc agaaattgcg
     1141 gaacaagata tcgttctgcc ggatatggca gtgggtatct acgattgggc gctcattgtc
     1201 gaccaccagc gtcatacagt ttctttgctg agtcataatg atgtcaatgc ccgtcgggcc
     1261 tggctggaaa gccagcaatt ctcgccgcag gaagatttca cgctcacttc cgactggcaa
     1321 tccaatatga cccacgagca gtacggcgaa aaatttcgcc aggtacagga atatctgcac
     1381 agcggtgatt gctatcaggt gaatctcgcc cagcgttttc atgcgaccta ttctggcgat
     1441 gaatggcagg cattccttca gcttaatcag gccaaccgcg cgccatttag cgctttttta
     1501 cgtcttgaac agggtgcaat tttaagcctt tcgccagagc ggtttattct ttgtgataat
     1561 agtgaaatcc agacccgccc gattaaaggc acgctaccac gcctgcccga tcctcaggaa
     1621 gatagcaaac aagcagaaaa actggcgaac tcagcgaaag atcgtgccga aaatctgatg
     1681 attgtcgatt taatgcgtaa tgatatcggt cgtgttgccg tagccggttc ggtaaaagta
     1741 ccagagctct tcgtggtgga acccttccct gccgtgcatc atctggtcag cactataacg
     1801 gcgcgactac cagaacagtt acacgccagc gatctgctgc gcgcagcttt tcctggtggc
     1861 tcaataaccg gggctccgaa agtacgggct atggaaatta tcgacgaact ggaaccgcag
     1921 cgacgtaatg cctggtgcgg cagcattggc tatttgagct tttgcggcaa catggatacc
     1981 agcattacta tccgcacgct gactgccatt aacggacaaa tatactgctc tgcgggcggt
     2041 ggaattgtcg ccgatagcca ggaagaagcg gaatatcagg aaacttttga taaagttaat
     2101 aagatattac gccaactgga gaagtaaaac gtggaatacc gtagcctgac gcttgatgat
     2161 tttttatctc gctttcaact tttgcgcccg caaattaacc gggaaaccct aaatcatcgt
     2221 caggctgccg tgttaatccc catcgtccgt cgaccacaac cggggttgtt gctgactcag
     2281 cgttcaattc atctgcgtaa acacgctgga caagtggcat tccctggagg tgcagtcgat
     2341 gacacggacg catcagttat cgccgccgcg ctgcgcgaag ctgaagaaga ggtcgctata
     2401 ccgccttccg ccgttgaagt tatcggcgtg ctgccgcccg tcgatagcgt aactggctat
     2461 caggtaaccc cagtggtcgg cattatcccg cccgatctgc cgtatcgcgc cagtgaagat
     2521 gaagtctcgg cggtgtttga aatgccgctc gcccaggcat tacatctggg tcgttatcac
     2581 cctttagata tctaccgccg tggcgattca catcgggtat ggctgtcctg gtacgaacag
     2641 tattttgtat ggggaatgac cgcaggcata attcgtgagc tggcgctgca aattggtgtg
     2701 aacccctgac tatacttatc tttacatcta caaaacacta cttgagacaa tcatcgcaat
     2761 attagttaaa tcgcggtttt tgattagttt aattcatgtg aatagttaag ccagtcgccg
     2821 cgttccctct tacactatgc gctgttatta gttcgttact ggaagtccag tcaccttgtc
     2881 aggagtatta tcgtgattag tctattcgac atgtttaagg tggggattgg tccctcatct
     2941 tcccataccg tagggcctat gaaggcgggt aaacagttcg tcgataatct ggtcgaaaaa
     3001 ggcttactgg atagcgttac tcgcgttgcc gtggacgttt atggttcact gtcgctgacg
     3061 ggtaaaggcc accacaccga tatcgccatt attatgggtc ttgcaggtaa cgaacctgcc
     3121 accgtggata tcgacagtat tcccggtttt attcgcgacg taaaagagcg cgaacgtctg
     3181 ctgctggcac agggacggca tgaagtggat ttcccgcgcg acaacgggat gcgttttcat
     3241 aacggcaacc tgccgctgca tgaaaacggt atgcaaatcc acgcctataa cggcgatgaa
     3301 gtcgtctaca gcaaaactta ttattccatc ggcggcggtt ttatcgtcga tgaagaacac
     3361 tttggtcagg atgctgccaa cgaagtgagc gtgccgtatc cgttcaaatc tgccaccgaa
     3421 ctgctcgcgt actgtaatga aaccggctat tcgctgtctg gtctcgctat gcagaacgaa
     3481 ctggcgctgc acagcaagaa agagatcgac gagtatttcg cgcatgtctg gcaaaccatg
     3541 caggcatgta tcgatcgcgg gatgaacacc gaaggtgtac tgccaggccc gctgcgcgtg
     3601 ccacgtcgtg cgtctgccct gcgccggatg ttagtttcca gcgataaact gtctaacgat
     3661 ccgatgaatg tcattgactg ggtaaacatg tttgcgctgg cagttaacga agaaaacgcc
     3721 gccggtggtc gtgtggtaac tgcgccaacc aacggtgcct gcggtatcgt tccggcagtg
     3781 ctggcttact atgaccactt tattgaatcg gtcagcccag acatctatac ccgttacttt
     3841 atggcagcgg gcgcgattgg tgcattgtat aaaatgaacg cctctatttc cggtgcggaa
     3901 gttggttgcc agggcgaagt gggtgttgcc tgttcaatgg ctgctgcggg tcttgcagaa
     3961 ctgctgggcg gtagcccgga acaggtttgc gtggcggcgg aaattggcat ggaacacaac
     4021 cttggtttaa cctgcgaccc ggttgcaggg caggttcagg tgccgtgcat tgagcgtaat
     4081 gccattgcct ctgtgaaggc gattaacgcc gcacggatgg ctctgcgccg caccagtgca
     4141 ccgcgcgtct cgctggataa ggtcatcgaa acgatgtacg aaaccggtaa ggacatgaac
     4201 gccaaatacc gcgaaacctc acgcggtggt ctggcaatca aagtccagtg tgactaatac
     4261 ttcttactcg cccatctgca acggatgggc gaatttatac ccgctttctc gtctgctgta
     4321 atattcccca ctacacttcc actgttgcgt caggcgtttg tcgccatacg cttacagggt
     4381 ggcccgcatg caaaaagcac agcggatcat taaaacctat cgccgtaatc gaatgattgt
     4441 ttgtacgatt tgcgcactcg ttacgctcgc ttcgaccctg agcgtgcgat ttatttcaca
     4501 gcgtaactta aatcaacaac gggtagtaca attcgccaat cacgctgtag aggaattaga
     4561 taaagtactg cttcccctac aggcaggtag cgaagtcttg cttccgctga ttggtctgcc
     4621 ctgctctgtc gcccatttgc cattacgtaa acaggcggca aaactccaaa ctgtgcgatc
     4681 cattggcctg gtgcaagacg gcacacttta ttgctccagc atttttggtt atcgcaatgt
     4741 gcccgtcgtg gacattctgg ctgaacttcc tgcaccgcaa ccacttttac gcctgacgat
     4801 cgaccgtgcc ctgattaaag gcagtccggt tttgattcaa tggacgcctg cagcaggcag
     4861 tagcaatgct ggggtcatag agatgattaa catcgactta ctgacggcaa tgctccttga
     4921 gccacaactg ccgcaaatca gtagcgccag cctgacggtg gacaaacggc atttgctcta
     4981 tggtaatggg ctggtagatt cccttccgca acctgaagac aatgaaaact atcaggtttc
     5041 ttcgcaaagc tttcctttta ccattaacgt aaatggtccg ggggctacgg cgctggcatg
     5101 gcactatctt ccaacacaat taccgctggc ggtgctgcta agtttactgg tgggctatat
     5161 cgcctggctg gcgaccgctt accggatgag cttttcccgc gaaattaatc tgggcctggc
     5221 gcaacatgag ttcgaattgt tctgtcagcc tttgcttaat gcgcgcagcc agcaatgtat
     5281 tggtgtagag attttgctgc gctggaataa tccgcgtcag ggctggattt caccggatgt
     5341 gtttattcct atcgcggaag aacatcattt aattgtgcca ctgacccgct atgtgatggc
     5401 agaaaccatt cgtcagcgcc atgttttccc gatgagtagt cagtttcatg ttggcattaa
     5461 cgtcgcaccc agccattttc gccgtggtgt gctgataaaa gatctcaatc agtactggtt
     5521 tagcgctcac ccgattcagc aactgatcct cgaaatcacc gaacgcgatg ccttactgga
     5581 tgttgattat cggattgccc gcgagctgca tcgtaaaaac gtcaaactgg cgattgatga
     5641 cttcggcacc ggcaacagct cgttttcctg gcttgaaaca ttacgtcctg acgtgctgaa
     5701 aattgataag tcatttaccg cagctatagg ttctgacgcg gttaactcga cggtgaccga
     5761 tatcatcatc gcgctggggc aaagactgaa tattgaactg gtggcggagg gcgtggaaac
     5821 gcaagaacag gcgaagtatt tgcgccgtca tggcgtgcat attttgcaag ggtatttgta
     5881 cgcacagccg atgccgctac gtgattttcc caaatggctg gcgggcagcc aaccgccgcc
     5941 cgcccggcat aatggacata tcacgcccat tatgtcgtta cgttaacgct tactcatctt
     6001 catcgtgcgc cggttgctct ttaacaatgc gaaccagatc aacacgataa tcattggctt
     6061 caaggatggt gatatgcagt ggccctacat caatcacatc gcccacacgg ggaatgtgac
     6121 catttgccga gatcacgagg cccgcgaccg tcgcgatatc atcgtcatcg gcaaggtgct
     6181 caacatcaag cgcctgctgc aaggcatgca aatctgtacc gccttttacc agccagccgt
     6241 taccatcggt aatgatttcc ggcgtttcgt cagcgtccgg gaattcaccc gcaatggctt
     6301 ccagcacatc cagcggcgtg accaagcctt gtaccacacc aaactcgttg gtcacgataa
     6361 caaagctccc gcgagcgcga cgcagcacgc ccagcaggtt gatcggatcg agggtttccg
     6421 ggacgataat cgccggagac gccgaagcaa tcgccgccac atcaacgccc tcttccagcg
     6481 ccaccagcag ttctttagca cgtacgatac cgatgatttc atccagttca ccgcgacata
     6541 ccgggaacag actgtgcggt gaagagagca gttgctcgcg gatttcatcg accccgagat
     6601 tagcgtcaac ccagcttatt tcaccgcgcg gcgtcatgat cccgcgcaga gaacgcgacg
     6661 ccagcgtcag tacgccgtta atcatgtacc gttcttcttc ggcaaatgca ccttccggga
     6721 tcggcatcgg gttatcggca tcgtgctgaa cattggcctg acgtttcccg cccatcaaac
     6781 gcaggatggc atcggcagta cgcgctcgca gcggcaaagt cgactggtga cgaataaagt
     6841 tgcgacgcgc aatctggtta aacacttcga taatgatcga gaagccaatc gcggcataca
     6901 ggtaaccttt cggaatgtgg aaaccgaaac cttctgccac cagactcaga ccaatcatta
     6961 acaggaagct cagacagagc accaccaccg tggggtgctg gttaacgaat cgcgtcagcg
     7021 gtttcgatgc cagcaacata accgccatcg caatcactac cgccgccatc atcaccggca
     7081 gatggttaac catccctact gcagtaatta ccgcatccaa cgagaagacg gcgtcaagga
     7141 tgacaatctg tgtgacgacc acccagaaac tggcgtagcc tttaccgtgg ccggaatcat
     7201 gatcgcagtt ttccagccgt tcatgcagtt cggttgttgc tttgaacagc aagaatatcc
     7261 ccccgaacaa cataatcaga tcgcgtccgg agaaggagaa atccatgacg gtaaatagcg
     7321 gtttggtcag cgtgaccatc catgaaatca gcgacagcag ccccagacgc ataatcagcg
     7381 ccagtgataa ccccagcaaa cgcgctttat cgcgttgttt tggcggcagt ttgtcagcaa
     7441 gaatggcgat gaagaccagg ttatcgatac ccagcacaat ttcgagaaca acaagcgtga
     7501 gtagccccgc ccaaattgag gggtccatta agaattccat gacaagctcc tgcttaagga
     7561 atagctattc gacgccagaa ataatgcagg cgtaacgaca aaatgcaaac gaaaggtgcg
     7621 gcatagagtg ccagaaaggc aggcgttaaa aggcctgatg ctgaaatgac gtcggtgacg
     7681 atccatactg cgggctactg ccctatactc catggttgtt aaacgggagt taaacatatc
     7741 agagacgcct ctgatttggc aaagatttac cttcctttgc aaacgaatgt gacaaggata
     7801 ttttaccttt cgaaatttct gataatcgaa agttaaatta cggatcttca tcacataaaa
     7861 taattttttc gatatctaaa ataaatcgcg aaacgcaggg gtttttggtt gtagccctta
     7921 tctgaatcga ttcgattgtg gacgacgatt caaaaataca tctggcacgt tgaggtgtta
     7981 acgataataa aggaggtagc aagtgaccat tgctattgtt ataggcacac atggttgggc
     8041 tgcagagcag ttgcttaaaa cggcagaaat gctgttaggc gagcaggaaa acgtcggctg
     8101 gatcgatttc attccaggtg aaaatgccga aacgctgatt gaaaaataca acgctcagtt
     8161 ggcaaaactc gacaccacta aaggcgtgct gtttctcgtt gatacatggg gaggcagccc
     8221 gttcaatgct gccagccgca ttgtcgtcga caaagagcat tatgaagtca ttgcaggcgt
     8281 taacattcca atgctcgtgg aaacgttaat ggcccgtgat gatgacccga gctttgatga
     8341 actggttgcg ctggcagtag aaacaggccg tgaaggcgtg aaagcactga aagccaaacc
     8401 ggttgaaaaa gccgcgccag cacccgctgc cgcagcacca aaagcggctc caactccggc
     8461 aaaaccaatg gggccaaacg actacatggt tattggcctt gcgcgtatcg acgaccgtct
     8521 gattcacggt caggtcgcca cccgctggac caaagaaacc aatgtctccc gtattattgt
     8581 tgttagtgat gaagtggctg cggataccgt tcgtaagaca ctgctcaccc aggttgcacc
     8641 tccgggcgta acagcacacg tagttgatgt tgccaaaatg attcgcgtct acaacaaccc
     8701 gaaatatgct ggcgaacgcg taatgctgtt atttaccaac ccaacagatg tagagcgtct
     8761 cgttgaaggc ggcgtgaaaa tcacctctgt taacgtcggt ggtatggcat tccgtcaggg
     8821 taaaacccag gtgaataacg cggtttcggt tgatgaaaaa gatatcgagg cgttcaaaaa
     8881 actgaatgca cgcggtattg agctggaagt ccgtaaggtt tccaccgatc cgaaactgaa
     8941 aatgatggat ctgatcagca aaatcgataa gtaacgtatt gtgttgatta tcattcagtt
     9001 ttcacactta agtcttacgt aaacaggaga agtacaatgg agattaccac tcttcaaatt
     9061 gtgctggtat ttatcgtagc ctgtatcgca ggtatgggat caatcctcga tgaatttcag
     9121 tttcaccgtc cgctaatcgc gtgtaccctg gtgggtatcg ttcttgggga tatgaaaacc
     9181 ggtattatta tcggtggtac gctggaaatg atcgcgctgg gctggatgaa catcggtgct
     9241 gcagttgcgc ctgacgccgc tctggcttct atcatttcta ccattctggt tatcgcaggt
     9301 catcagagca ttggtgcagg tatcgcactg gcaatccctc tggccgctgc gggccaggta
     9361 ctgaccatca tcgttcgtac tattaccgtt gctttccagc acgctgcgga taaggctgct
     9421 gataacggca acctgacagc gatttcctgg atccacgttt cttctctgtt cctgcaagca
     9481 atgcgtgtgg ctattccggc cgtcatcgtt gcgctgtctg ttggtaccag cgaagtacag
     9541 aacatgctga atgcgattcc ggaagtggtg accaatggtc tgaatatcgc cgggggcatg
     9601 atcgtggtgg ttggttatgc gatggttatc aacatgatgc gtgctggcta cctgatgccg
     9661 ttcttctacc tcggcttcgt aaccgcagca ttcaccaact ttaacctggt tgctctgggt
     9721 gtgattggta ctgttatggc agtgctctac atccaactta gcccgaaata caaccgcgta
     9781 gccggtgcgc ctgctcaggc agctggtaac aacgatctcg ataacgaact ggactaacag
     9841 gtgagcgaaa tggttgatac aactcaaact accaccgaga aaaaactcac tcaaagtgat
     9901 attcgtggcg tattcctgcg ttctaacctc ttccagggtt catggaactt cgaacgtatg
     9961 caggcactgg gtttctgctt ctctatggta ccggcaattc gtcgcctcta ccctgagaac
    10021 aacgaagctc gtaaacaagc tattcgccgt cacctggagt tctttaacac ccagccgttc
    10081 gtggctgcac cgattctcgg cgtaaccctg gcgctggaag aacagcgtgc taatggcgca
    10141 gagatcgatg acggtgctat caacggtatc aaagtcggtt tgatggggcc gctggctggt
    10201 gtaggcgacc cgatcttctg gggaaccgta cgtccggtat ttgcagcact gggtgccggt
    10261 atcgcgatga gcggcagcct gttaggtccg ctgctgttct tcatcctgtt taacctggtg
    10321 cgtctggcaa cccgttacta cggcgtagct tatggttact ccaaaggtat cgatatcgtt
    10381 aaagatatgg gtggtggctt cctgcaaaaa ctgacggaag gggcgtctat cctcggcctg
    10441 tttgtcatgg gggcattggt taacaagtgg acacatgtca acatcccgct ggtggtttcg
    10501 accatcaccg ggcaggatgg acagacccgt gtcaccaccg tgcaaactat tcttgaccag
    10561 ttaatgccag gccttgtacc actgctgctg acatttgctt gtatgtggct gctgcgcaaa
    10621 aaagttaacc cgctgtggat catcgttggc ttcttcgtca tcggtatcgc tggttacgct
    10681 tgcggcctgc tgggactgta agactgttgt acactaccgg ggccttttgg ccccgttttt
    10741 ttatctggag gattaatgac aatcacggac ctggtactga ttcttttcat cgccgcactc
    10801 ctggccttcg cgatctacga tcagttcatc atgccccgcc gtaacggccc caccctgctg
    10861 gcaattcctt tgctccgccg tggtcgcatc gatagcgtta tcttcgtcgg attgattgtc
    10921 attcttatct ataacaacgt cacgaatcat ggcgcgctaa taacaacatg gttattaagt
    10981 gcactggctc tgatgggttt ttatatattc tggatccgca ttccgaagat catctttaaa
    11041 caaaaaggtt ttttcttcgc caatgtctgg attgaatata gccgaatcaa agcgatgaac
    11101 ttgtcggaag atggcgtgtt ggtgatgcaa ttagaacagc gtcggctgtt aatccgcgtt
    11161 cgaaatatcg acgatctgga aaaaatttat aagcttctcg
//
LOCUS       DADMGC010000126        11183 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.126, whole genome shotgun sequence.
ACCESSION   DADMGC010000126 DADMGC010000000
VERSION     DADMGC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.126"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="JNZ73_003331"
     CDS             complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="JNZ73_003331"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HAY8733502.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFSWRCDITSEQELSALADFAVSKLGKVDILVNNVGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITP"
     gene            complement(721..1749)
                     /locus_tag="JNZ73_003332"
     CDS             complement(721..1749)
                     /locus_tag="JNZ73_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HAY8733503.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1924..3516
                     /locus_tag="JNZ73_003333"
     CDS             1924..3516
                     /locus_tag="JNZ73_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HAY8733504.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3526..4698
                     /gene="malY"
                     /locus_tag="JNZ73_003334"
     CDS             3526..4698
                     /gene="malY"
                     /locus_tag="JNZ73_003334"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HAY8733505.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4801..5802
                     /gene="add"
                     /locus_tag="JNZ73_003335"
     CDS             4801..5802
                     /gene="add"
                     /locus_tag="JNZ73_003335"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAY8733506.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5838..6878)
                     /locus_tag="JNZ73_003336"
     CDS             complement(5838..6878)
                     /locus_tag="JNZ73_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY8733507.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7121..7246
                     /gene="blr"
                     /locus_tag="JNZ73_003337"
     CDS             7121..7246
                     /gene="blr"
                     /locus_tag="JNZ73_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HAY8733508.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7519..7734
                     /gene="ydgT"
                     /locus_tag="JNZ73_003338"
     CDS             7519..7734
                     /gene="ydgT"
                     /locus_tag="JNZ73_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HAY8733509.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7820..8260
                     /locus_tag="JNZ73_003339"
     CDS             7820..8260
                     /locus_tag="JNZ73_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HAY8733510.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8337..8918
                     /gene="rsxA"
                     /locus_tag="JNZ73_003340"
     CDS             8337..8918
                     /gene="rsxA"
                     /locus_tag="JNZ73_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAY8733511.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFATIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8918..9496
                     /gene="rsxB"
                     /locus_tag="JNZ73_003341"
     CDS             8918..9496
                     /gene="rsxB"
                     /locus_tag="JNZ73_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HAY8733512.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9489..>11183
                     /gene="rsxC"
                     /locus_tag="JNZ73_003342"
     CDS             9489..>11183
                     /gene="rsxC"
                     /locus_tag="JNZ73_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY8733513.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIAR"
BASE COUNT         2599 a         2882 c         2815 g         2887 t
ORIGIN      
        1 tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa tgccattcac
       61 ccgaatattt ttttcaccca ggtcaaatgc catatttctg accagatgac tggccgcagc
      121 tttagatgat gcataggaag tcatgtttat atttttattt tctgccgcca tagaagtgat
      181 ggtcagaata acgccaccgc catttttttc catttctggc gcaacaagtt gtgacagatg
      241 gaaaaaagaa aacacattca gttcataagc acggcgaaaa tctgccattg gcatatcaaa
      301 cggtttagga ccaccgccac cgacgttgtt aaccaggata tcaactttac ccagtttact
      361 gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcac aacgccagga
      421 aaatgcctga ccacccagtt gttgaatttc gtctacaaca tggttagctg cgtcggcgtt
      481 aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga tttctttacc
      541 aatacctgca cccgcacctg tgatgatggc gcattttccg tcgagtctca ggttgtcaga
      601 attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc ctgtgtgcca
      661 cattttttat cattaacctt gaggaaagga cactaccaag tatagcagat aaagagagga
      721 ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg tctcttcatg
      781 ggtgattttt tgcatcatgc gatccgcaag tgtgatacca agttcccgcg ctggcgtgct
      841 ggcccaggta acaggtatat catcaagtgt ggttggtgtc gcatcggtaa atgccgccag
      901 agaaacctgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc gccctgcttt
      961 cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca cggcactgat
     1021 ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct ggctggaagt
     1081 gcactccaac acccaatcgc tgtgaaacgg caggccaaat tttagtagag ttgcacaata
     1141 gccccccacc cgttctgcac gggttaatga ggaactttgc cctcccagcc aggcgatccg
     1201 ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca tgttgtccgg
     1261 gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca ccgggatagc
     1321 tttttcttct gccatccgcc gcaggtcatc gctgcttcct gcagccccgg caattaccac
     1381 accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct cgccgtcttt
     1441 accgccgtga agcaaaaaaa ccatccgtcc ctgcgcttcc agagcttccg tcaatccggc
     1501 cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa tgacgccgct
     1561 ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct cttcaatggc
     1621 ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccactaa gcaccagcga
     1681 aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta tttttttggc
     1741 ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt ttacctttta
     1801 tttatcttat acccgctatt atcgttgcgt aatgtgattt atgactcact aaaatttgat
     1861 aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac gaggagtcgt
     1921 tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca gcagttaggc
     1981 aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct cggcattggt
     2041 agttctctta gcagccatga tgtcataacc ctgatcccgg tcctgggcaa ccccgtgttg
     2101 caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt cctgcctgtc
     2161 atgttctgta tcgccatccc gctgggcctg gcacgcgaaa ataaaggcgt agcggcattc
     2221 gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt gaccaataaa
     2281 ggcattctgc caaccacaga tgccgccgtt ctgaaagcca ataacatcca gagcattctt
     2341 gggatccagt cgatcgatac cgggatcctc ggtgcggtga tcgccggtat tatcgtctgg
     2401 atgctgcatg agcgtttcca taatatccgc ctgccggatg cgctggcatt cttcggcggt
     2461 acgcgcttcg taccaattat ctcctcgctg gtgatgggcc ttgtcggcct ggtgattcca
     2521 ttagtctggc cgattttcgc catgggtatt agcggcttgg gccatatgat aaacagcgcg
     2581 ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttacc gtttggtctg
     2641 catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca ggaggtctgc
     2701 ggtcaaaccg tcagcggcgc actgaccatc ttccaggcgc aattgagttg cccgaccact
     2761 cacggttttt ctgaaagcgt cacgcgtttc ctttcgcaag gtaaaatgcc tgcgtttctc
     2821 ggcggtctgc caggtgcagc gttagctatg tatcactgcg cgcgcccgga aaatcgccat
     2881 aaaattaaag gtctgctgat ttctggcctg atcgcctgcg tcgttggcgg cactaccgaa
     2941 ccgctggaat tcctgttcct gttcgtagcg ccagttctgt atgtcatcca cgcgctgtta
     3001 accggcctcg gcttcaccgt catgtctgtg ctcggcgtca ccatcggtaa taccgacggc
     3061 aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa gtggtacatg
     3121 gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg tttcgctatc
     3181 acccgcttca atctgaaaac cccggggcgc gatagcgaag ttgccagctc aatcgaaaaa
     3241 gccgttgccg gtgcgccggg taaatcaggt tacaacgttc ctgcaatcct cgaagcatta
     3301 ggcggtgccg acaatattgt cagcctcgat aactgcatta cccgtctgcg tttgtctgtg
     3361 gaagatatgt cgcttgttaa tgtgcaagca ctgaaggaca atcgggcaat tggcgtagta
     3421 caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc agtaaaagat
     3481 gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt cgatttttca
     3541 aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc tgaccgtttc
     3601 ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac tgccccctgc
     3661 attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag ccgctggaaa
     3721 aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta caccgcaatt
     3781 gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga actgattcgt
     3841 cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga cgcattttac
     3901 aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa gcaggctgac
     3961 ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga atgtaaaatt
     4021 atgctcctgt gtaacccaca gaatcctacc gggaaagtgt ggacgtgcga tgagctggag
     4081 atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga aatccatatg
     4141 gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg gggagactgg
     4201 gcgttgctga cgtcgggatc gaaaagtttc aatattcccg cgctgaccgg tgcttacggg
     4261 attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg acgtgatggg
     4321 ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca gcaaggtgca
     4381 ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat ctcagataaa
     4441 atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta tctggcctgg
     4501 cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct tatcgagcaa
     4561 gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg ttttgtccgt
     4621 ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt aataaacgcc
     4681 atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa tcgttgcgca atcgtggatt
     4741 tttaccctgc tttgttttta taatggtgcg cacttttata tccagaaaaa gagtgcgacc
     4801 atgattgata ccaccctgcc attaactgat atccatcgcc accttgatgg caacattcgt
     4861 ccccagacca ttcttgaact tggccgccag tataatatct cgcttcctgc acaatccctt
     4921 gaaacactga ttccccacgt tcaggtcatt gccaacgaac ccgatctggt gagctttctg
     4981 accaaacttg actggggcgt taaagttctc gcctctcttg atgcctgccg ccgcgtggca
     5041 tttgaaaaca ttgaagatgc agcccgtaac ggcctgcgct atgtcgagct gcgtttttca
     5101 ccaggctaca tggcaatggc acataagctg cctgtagcgg gtgttgtcga agcggtgatc
     5161 gatggcgtac gtgaaggttg ccgcaccttt ggtgtgcagg cgaagcttat cggcattatg
     5221 agccgaacct tcggcgaagc cgcctgtcag caagagctgg aggccttttt agcccaccgt
     5281 gaccagatta ccgcacttga tttagctggt gatgaacttg gattcccggg aagtctgttc
     5341 ctttctcact tcaaccgcgc gcgtgatgcg ggctggcata ttaccgtcca tgcaggcgaa
     5401 gctgccgggc cggaaagcat ctggcaggcg attcgtgaac tgggtgcgga gcgtattgga
     5461 catggcgtaa aagccattga agatcgggcg ctgatggatt ttctcgccga gcaacaaatt
     5521 ggtattgaat cctgtctgac ctccaatatt cagaccagca ccgtagcaga gctggctgca
     5581 catccgctga aaacgttcct tgagcatggc attcgtgcca gcattaacac tgacgatccc
     5641 ggcgtacagg gagtggatat cattcacgaa tataccgttg ccgcgccagc tgctgggtta
     5701 tcccgtgagc aaatccgcca ggcacagatt aatggtctgg aaatggcttt cctcagcgca
     5761 gaggaaaaac gcgcactgcg agaaaaagtc gccgcgaagt aacaaaaatg gatggtgcaa
     5821 attgcaccat ccatttttca cgcaaggcac aacgtcgcgc gatgtttggc ggattcgatg
     5881 cccagctcaa tcaactccat tacctggatt gcctggcttg ccggaaccgg attttcacca
     5941 tcgccattta acgcatcacg aatcgccgca tagtaagccg gataattccc aggcaccgtc
     6001 aacagcgttt cttcgacacg ttcctcacct tccacgcggg taagtacgcc atcacgcata
     6061 tcgtagcccc agtcttcctg cggcagacgc tcgccatttt tcagacgttc ttcctgtgga
     6121 tcgaggccat atttcacata actgcctcgg gatccatgca cgatataccg tgctgactca
     6181 gcggctgcca gcatggtacc gtgtaaaatg actcgccgct gtggatagga caagatggcg
     6241 tggaaataat cggtcgactg cgctccgggc cgtaactgtg ccaaatctac cgtcatgctg
     6301 accggtaaac caaatagcgt aatggcctga tcaagaagat gcggtgctaa atcgtaccag
     6361 ataccgctgc ctgggccgcc ctgttcacgc caacgatcgc gcacctgcgg acggaagcgg
     6421 tcaaaatgag actcaaagta agcaacttca cccagcacgc cttccgcgag caaacctttt
     6481 agcgtcaaga aatcgctatc ccaacgacgg ttatggaata cagacagcac acgccccagg
     6541 ctttttgcca gcgcatccag ctctcgcgct tgtgacagtg tcacggtaaa gggtttatca
     6601 acgaccacat gtttacccgc ctcaagcgcc gctttggcta acgggaaatg ggtatcgttg
     6661 ggtgtaggaa tgacaatcag gtctatgttg ggatcgttaa acagatgctt cggctcagag
     6721 acaaccgtaa ccgttggcca gtcggctttt acttttgttt catcactgct ggagattacc
     6781 gccagttcca gcccgggcgt gcccgcaatc aggggcgcat ggaaggtttt gctcgcataa
     6841 ccatacccaa tcaacccaac acggatgttg tcgctcatgt cattgcctct cattgaagta
     6901 tgatggctat ttgacactat cctttaccca cgctcaacag tttaataacc tgccagcaat
     6961 aagggatgtt gtttaactta agtcaaaaaa atagcgaatt ttccaacgac aaaagctaaa
     7021 tatcgcaaaa acctcagtaa aaatcttgct ggagctatta ttgctaagta acatttaccc
     7081 cctgaagtta atggatcaat caagagagat gtgggctgta atgaatcgtc ttattgaatt
     7141 aacaggttgg atcgttcttg tcgtttcagt cattcttctt ggcgtggcga gtcacattga
     7201 caactatcag ccacctgaac agagtgcttc ggtacaacac aagtaagctc tgcacttgtg
     7261 gagcgacatg ctgcccgtcc gggtgcatgt tttcacttgt cggatattaa accaggaatt
     7321 tattatcttg ttcgatgttg ttggtgattg tcagggatag taaagcgcgt tactcttttc
     7381 agagcagttt ttcatccctg aatatttgcc atcagcgcat tcttattatt gtaataacac
     7441 aagcgttcac ttttggtgaa attttatcat cactaataat acctacccct ttggtattaa
     7501 ttattttatg gaccttttat gactgttcag gactacttat taaaatttcg caaaatcagt
     7561 tcactcgaaa gtctggaaaa actctacgac catcttaatt acaccctgac ggacgatcag
     7621 gaactgatca atatgtatcg tgctgccgat caccgtcgcg cagagctggt ttctggcggg
     7681 cgtttgtttg acctcggcca ggtaccgaag tccgtctggc actatgtcca ataaagaaag
     7741 tagcgatcgg ttataaaagc cttataataa cgcccctaaa attagatttg cctgtttgcg
     7801 tcgctattct ggagaaccta tgaccaccac aacgccacaa cgtattggag gctggttgct
     7861 tggcccgttg gcctggcttt tagtcgctct gttgagtacg acgctggcgt tgttgctgta
     7921 taccgctgcg ttatcttctc cgcaaacatt tcaaacgctc ggcggacaag cactgacgac
     7981 acaaatctta tggggcgttt cttttattac cgctatcgcc atgtggtatt acacactgtg
     8041 gttgaccatc gctttcttta aacgtcggcg ttgtgttcct aaacactata ttatctggct
     8101 gcttatttcc gtattgctgg cagtaaaagc attcgctttt tcacccgtgg aagttggcat
     8161 agccgttcgc cagttgctgt ttactttgtt ggcaaccgca ttgatcgtcc cctatttcaa
     8221 acgctcgtcg cgggtaaaag cgacgtttgt gaatccgtaa taaccttaca gttaacctgt
     8281 tgtcgcctgc tctggattaa cggataatag gcggcttttt tatttcaggc cgaaaaatga
     8341 ctgactacct gttactgttt gtcggaactg tactggtcaa taactttgta ctggtcaagt
     8401 ttctcggtct ctgtccgttt atgggggttt ccaaaaaact ggaaaccgcg atgggcatgg
     8461 ggctggcaac aacgtttgtg atgacgctga cgtctatttg cgcctggctt atcgatacgt
     8521 ggattttgat cccacttaat ctgatttacc tacgcaccct ggcatttatt ctggtgattg
     8581 ctgtggtcgt gcagttcacc gagatggtgg tgcgcaaaac cagcccggtg ctttaccgtc
     8641 tgctggggat ttttttgccg cttatcacca ccaactgtgc agtgctcggc gtggcgttgc
     8701 tgaatatcaa tctcgggcac aatttcttgc agtcggcgct gtacggtttt tccgccgctg
     8761 tcggtttctc gctggtgatg gtgctcttcg ccaccatccg cgaacgcctt gctgtggctg
     8821 atgtcccggc accttttcgc ggtaatgcca ttgcgttaat taccgcaggt cttatgtctc
     8881 tggcctttat gggctttagt ggtttggtga agttgtaatg aatgctatct ggattgccgt
     8941 tgccgccgtg agcctgctgg gcctggcgtt tggcgccatt ctgggttatg cctcccgccg
     9001 ttttgcggtg gaagacgatc cggtcgttga gaaaattgac gaaatcttac cgcagagcca
     9061 gtgtggtcag tgcggttatc ccggctgtcg cccctacgcg gaagccatca gctgtaacgg
     9121 tgaaaaaatc aaccgttgcg ccccaggtgg cgaagctgtg atgctaaaaa ttgccgagtt
     9181 gcttaatgtc gagccgcagc cgctggatgg cgaagcgcaa gagctaacgc ctgcgcggat
     9241 ggtggcggtt attgatgaaa ataactgtat tggctgcact aaatgtattc aggcttgtcc
     9301 ggtagacgcc atcgttggcg ctacccgtgc catgcatacg gtaatgagtg atctctgtac
     9361 gggctgcaat ttatgtgttg atccgtgccc gacgcactgc atctcgttgc aaccggtcgc
     9421 agaaacacct gactcctgga aatgggatct gaacaccatt cccgtgcgta tcattcccgt
     9481 ggaacaccat gcttaagtta ttctctgcat tcagaaaaaa taaaatctgg gatttcaacg
     9541 gcggcatcca tccaccggag atgaaaaccc agtccaacgg tacccccctg cgccaggtac
     9601 cactggcgca gcgttttgtt attccactga aacagcatat tggcgctgaa ggtgagttgt
     9661 gcgttagcgt cggcgataaa gtattgcgcg gccagccgct tacccgtggt cgcggcaaaa
     9721 tgctgcctgt tcacgcgccc acctcgggta ccgttacggc tattgcgccc cactctacgg
     9781 ctcatccttc agctttagct gaattaagcg tgattattga tgccgatggt gaagactgct
     9841 ggatcccgcg cgacggctgg gtcgattatc gctctcgcag tcgcgaagag ttaatcgagc
     9901 gcatacatca gtttggtgtt gccgggctgg gcggtgccgg attcccgaca ggcgttaaat
     9961 tgcagggtgg tggagataag attgaaacgt tgattatcaa cgctgctgag tgcgagccgt
    10021 acattaccgc cgatgaccgt ttgatgcagg attgcgcggc tcaggtcgta gagggtattc
    10081 gcattcttgc gcatattctg cagccacgcg aaattcttat cggcattgaa gataacaaac
    10141 cgcaggcgat ttccatgctg cgcgcggtgc tggcggactc taacgatatt tctctgcggg
    10201 tgattccaac caaatatcct tctggcggtg ctaaacaatt aacctacatt ctgaccggga
    10261 agcaggttcc acatggcggg cgttcatccg atatcggcgt attaatgcaa aacgtcggca
    10321 ctgcttatgc agtgaaacgt gccgttattg atggcgagcc gattaccgag cgtgttgtaa
    10381 ccctgactgg cgaagcaatc gctcgcccgg gcaacgtctg ggcacggctg ggaacgccag
    10441 tgcgtcattt attgaatgat gccggattct gcccctctgc cgatcaaatg gtgattatgg
    10501 gtggcccgct aatgggcttt accttgccat ggctggatgt cccggtcgta aagattacca
    10561 actgtctgtt ggctccctct gccaatgaac ttggcgaacc acaggaagaa caaagctgca
    10621 tccggtgtag cgcctgtgct gacgcctgcc ctgtggatct tttgccgcaa cagttgtact
    10681 ggttcagcaa aggtcagcaa cacgataaag ctaccacgca taacattgcc gactgcattg
    10741 aatgtggggc ttgcgcgtgg gtttgcccga gcaatattcc cttggtgcaa tatttccgtc
    10801 aggaaaaagc tgaaattgcg gctattcgtc aggaagaaaa gcgcgccgca gaagccaaag
    10861 cgcgtttcga agcgcgccag gctcgtctgg agcgcgaaaa agcggctcgc cttgaacgac
    10921 ataagagcgc agccgttcaa cctgcagcca aagataaaga tgcgattgct gccgctctgg
    10981 cgcgggtgaa agagaaacag gcccaggcta cacagcctat tgtgattaaa gcgggcgaac
    11041 gcccggataa cagtgcaatt attgcagcac gggaagcccg taaagcgcaa gccagagcga
    11101 aacaggcaga actggagcaa actaacgacg cagcaaccgt tgctgaacca cgtaaaactg
    11161 ccgttgaagc agctatcgcc cgc
//
LOCUS       DADMGC010000127        11177 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.127, whole genome shotgun sequence.
ACCESSION   DADMGC010000127 DADMGC010000000
VERSION     DADMGC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.127"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            14..1280
                     /locus_tag="JNZ73_003343"
                     /pseudo
     CDS             14..1280
                     /locus_tag="JNZ73_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237023.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            1332..2487
                     /locus_tag="JNZ73_003344"
                     /pseudo
     CDS             1332..2487
                     /locus_tag="JNZ73_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(2453..2575)
                     /locus_tag="JNZ73_003345"
     CDS             complement(2453..2575)
                     /locus_tag="JNZ73_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733514.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            2778..3839
                     /locus_tag="JNZ73_003346"
     CDS             2778..3839
                     /locus_tag="JNZ73_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HAY8733515.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLINKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTE"
     gene            3933..4463
                     /locus_tag="JNZ73_003347"
                     /pseudo
     CDS             3933..4463
                     /locus_tag="JNZ73_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            4501..6873
                     /locus_tag="JNZ73_003348"
     CDS             4501..6873
                     /locus_tag="JNZ73_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY8733516.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNTPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            6918..9713
                     /locus_tag="JNZ73_003349"
     CDS             6918..9713
                     /locus_tag="JNZ73_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY8733517.1"
                     /translation="MEIIMRNLCFLLTLVATLLLPGRLIAAALQQDEKLITGQLDNGL
                     RYMIYPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGAKTWPGNKVIETF
                     ESMGLRFGLDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAE
                     RGVISEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQR
                     WYQPNNMTFIVVGDIDSKEALALIKDNLSKFPANKAAENRVWPTKAENHLRFNIINDK
                     ENRVNGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTA
                     RSVKIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWL
                     KNAVDQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQL
                     RKNQDAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQ
                     AEISSKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIA
                     LANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFLLIN
                     QRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIA
                     QFTAADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPL
                     TRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIRE
                     QASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQEL
                     NEYQQNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTIENVNTAVK
                     QYLSHPVNTYTGVLLPK"
     gene            10075..>11177
                     /locus_tag="JNZ73_003350"
     CDS             10075..>11177
                     /locus_tag="JNZ73_003350"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY8733518.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICT"
BASE COUNT         3230 a         2511 c         2474 g         2962 t
ORIGIN      
        1 ccgtgccgca ataatgaccg gtcgagctcc cgcccgcttt ggtgtctatt ccaataccga
       61 tgctcaggat ggtattccgc taacagaaac tttcttgcct gaattattcc agaatcatgg
      121 ttattacact gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc
      181 ggaagataaa caaacgcgtg actatcatga caacttcacc acattttctg cggaagaatg
      241 gcaacctcaa aaccgtggct ttgattactt tatgggattc cacgctgcag gaacggcata
      301 ttacaactcc ccttcactgt tcaaaaatcg tgaacgtgtc cccgcaaaag gttatatcag
      361 cgatcagtta accgatgagg caattggcgt tgttgatcgt gccaaaacac ttgaccagcc
      421 ttttatgctt tacctggctt ataatgctcc gcacctacca aatgataatc ctgcaccgga
      481 tcaatatcag aagcaattta ataccggtag tcaaacagca gataactact acgcttccgt
      541 ttattctgtt gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa acggacagta
      601 tgacaataca attattctct ttacctccga taatggtgcg gttatcgatg gtcctctgcc
      661 gctgaacggg gcgcaaaaag gctataagag tcagacctat cctggcggta ctcaccaccc
      721 caatgtttat gtggtggaaa ggaaaacttc aacccggtaa ttatgacaag ctgatttccg
      781 caatggattt ctacccaaca gctcttgatg cagccgatat cagcattcca aaagacctta
      841 agctggatgg tgtttccttg ctgccctggt tgcaagataa gaaacaaggc gagccacata
      901 aaaatctgac ctggataacc tcttattctc actggtttga tgaggaaaat attccattct
      961 gggataatta ccacaaattt gttcgccatc agtcagacga ttacccgcat aaccccaaca
     1021 ctgaggactt aagccaattc tcttatacgg tgagaaataa cgattattcg cttgtctata
     1081 cagtagaaaa caatcagtta ggtctctaca aactgacgga tctacagcaa aaagataacc
     1141 ttgccgccgc caatccgcag gtcgttaaag agatgcaagg cgtggtaaga gagtttatcg
     1201 acagcagcca gccaccgctt agcgaggtaa atcaggagaa gtttaacaat atcaagaaag
     1261 cactaagcga agcgaaataa ctaaaccttc atgcggcgga tttttccgcc gccttattga
     1321 gcgagatagc aatgcacgtt acagccaagc cctccagttt tcaatgtaat ctcaaatgtg
     1381 attactgttt ttaccttgaa aaagagtcgc agtttactca tgaaaaatgg atggatgaca
     1441 gcactctgaa agagttcatc aaacaatata tcgaagcgtc tggcaatcag gtctatttta
     1501 cctggcaagg cggtgaaccc actctggctg gcctggattt tttccgtaaa gttattcact
     1561 atcaacaacg ctatgcaggc caaaaacgta tttttaatgc attacaaacg aatggcattt
     1621 tattgaataa tgaatggtgt gcctttctca aagaacatga ttttctggtt ggtatctcga
     1681 tcgatggccc ccaggagtta catgaccgtt acagacgcag taattcaggt aacggtactt
     1741 ttgcaaaagt gatagcagcc atcgagcgtc tgaaatcata tcaagtagag tttaatacgt
     1801 taaccgtcat taataacgtt aatgtccatt accctcttga ggtttatcat tttttaacat
     1861 ctatcggcag taaacatatg caatttatcg aattgctaga aaccgggacg ccgaatattg
     1921 atttcagtgg tcatagtgag aacacattcc gtatcattga tttttctgtg cctcccacgg
     1981 cttatggcaa gtttatgtca accattttta tgcaatgggt taaaaacgat gtgggtgaaa
     2041 ttttcattcg tcagtttgaa agctttgtca gccgtttttt ggggaatggg catacaagtt
     2101 gtattttcca ggagtcctgc aaggataatc tggttgttga aagtaatgga gacatttacg
     2161 aatgcgacca ttttgtctat ccacagtaca aaattggaaa cattaataaa tctgaactca
     2221 aaacgatgaa cagtgtacaa ctgacagcgc aaaaaacgga tttcagcgaa atgtcagcaa
     2281 tgtgcatata aacctatctg caatggcggt tgtcctaagc atcgtattac taaagtaaac
     2341 aatgagactg tttcttattt ttgcgaaggt tataaaatcc ttttttcaac catggtacct
     2401 tatatgaacg ccatggttga gttagctaag aacagagtac cgctttacca cattatggat
     2461 gttgcaaaac aaatggagaa taattaataa cgctctcttt taactccatt cttagcctcc
     2521 ttcttatgac cttaacaagg cacccgacat aaattcctgt cgggtgtttc gtcatcatca
     2581 tcttcccttg cgaaaatcct ggcttacgtg gtatgcgtca gggcattagc aatacccttg
     2641 attatcaata gcataaccat aaccactgaa aattaaaaat ttctatatat ttcatattgt
     2701 taaatgatac ttaaataaat ttcatcagta aatcattgcc atagataatc attggatggc
     2761 acaatcaatt ttcgctaatg ataaccattc ccattacgtt ttgtatgttg atagcaaagt
     2821 atctctgtct gctgaagccc ttttggctgc gtaagaataa taaaacttca gtgttgttaa
     2881 tcattattat cctcgcaatg atccttggcg tggttaaaat tcaggtctgg ttaaacgact
     2941 ggaataatga ttttttcaat gcgctaagcc agaaagaaac cgataagctt tggcagcttg
     3001 ttctttggtt ccctgccctg ctggggatct ttgtattaat atccgtaaac aaaacatggt
     3061 taattaaact actgactatt cgctggcgcg aatggttaac agattactac cttaatcgct
     3121 ggtttgcaga taaaaactac tacttcacgc agatctacgg cgagcataaa aatacagata
     3181 accctgacca acgtatcgct gaagacattc tcttactgat caacaaaacg ctcagtcttt
     3241 catttggctt catccagtca cttagcatgc tgatcacctt taccgttatt ctttggcaaa
     3301 gcgcgggtac gctctcattc actgttggtg gaacagaatg gaatatccag gggtatatgg
     3361 tctataccgt cgtgctcatt gtgatcggtg gaactttatt tacccataag gtgggtaaac
     3421 gtattcgtcc gcttaatgtg gaaaaacaac gtagcgaagc gacatttcga actaatcttg
     3481 tgcagcataa taagcaggca gaactgattg cgctaagcaa tgctgagtct ctacaacgcc
     3541 aggagttgcg cgacaatttt cataccatta aagagaactg gcatcgttta atgaatcgtc
     3601 agcggtggct tgattactgg caaaatattt attcgcgctc gcttagcgtt cttccctact
     3661 ttctgttatt gccgcagttt attagcgggc agatcaatct gggcggactg atgaaatcgc
     3721 gccaggcatt tatgctagta tcgaacaatt taagctggtt tatttataaa tatgacgaac
     3781 ttgctgaact ggctgcggtt atcgatcgct tgtatgagtt ccatcaactc actgaatagc
     3841 gccctacgaa taagcctaaa aattgccaac atgcggtaca agtggctaat gcgagtattc
     3901 gtacgcctga taataagatc atattagaga acttgaactt tcatgtttcg ccaggcaaat
     3961 ggctattact gaaaggctac tctggtgcgg gaaaaaccac actgcttaaa acattatccc
     4021 actgctggcc gtgatttaaa ggtgatattt cttctcctgc tgacagttgg tatgtgtcac
     4081 aaacaccgtt aatcaaaagc ggcttactga aagagattat ttgtaaagca cttcccctgc
     4141 ccgtagacga taaatcgttg agcgaagtac tgcatcaggt tggtctgggg aaattggctg
     4201 cgcgtattca tgaccacgat cgctggggag atattctttc cagcggcgaa aaacaacgta
     4261 tcgccctggc acgattaatt ttacgacgtc cgaaatggat atttcttgac gaaactacct
     4321 ctcatcttga ggaacaagag gctatacgct tactgcgttt agtgcgtgaa aaactaccca
     4381 caagcggcgt cattatggtt acacatcaac ccggtgtctg gaacctggct gatgatattt
     4441 gtgacattag cacggtttta taacaacaat aaaagccctg ctgtacggag atataaataa
     4501 atgaagcgag ttcttattcc tggcgtcatt ttatgtggcg ctgatgtggc gcaggccgtc
     4561 gatgacaaaa acatgtacat gcattttttt gaagagatga cggtctatgc tcctgtccct
     4621 gtacccgtaa acggcaacac gcattacacc agtgaaagca tcgagcgttt accgaccggg
     4681 aatggcaata tcagcgatct gctgagaacc aaccctgcgg tacgcatgga ttcaacgcaa
     4741 agtacctcgt tgaaccaggg agatattcgc cctgagaaaa tctctattca cggtgcgtcg
     4801 ccctaccaga atgcctattt gattgacggt attagtgcaa ctaataacct gaacccagcg
     4861 aatgagtccg atgccagtag tgcaaccaat attagcggga tgtcacaggg gtattatctt
     4921 gatgtcagct tactggacaa tgtgacgctt tatgacagtt ttgtgccggt tgaatttggt
     4981 cgcttcaatg gcggggtaat tgatgcaaag atcaaacgct tcaacgctga tgatagcaag
     5041 gtgaaattgg gttatcgcac tacgcgtttg gactggttaa catcgcatat cgatgagaat
     5101 aacaagagcg catttaatca aggttcttca ggaagtacct atttctctcc agattttaaa
     5161 aagaactttt ataccttgtc gtttaatcag gagctggctg ataactttgg cgttaccgcc
     5221 ggtttatcgc gccgccagtc tgatatcacc cgcgcggatt atgtttcgaa tgacggcatt
     5281 gtcgccggtc gggcacagta taaaaacgtt atcgatactg cattgagcaa atttacctgg
     5341 tttgccagcg accgctttac ccacgattta accttaaaat ataccggctc cagccgtgat
     5401 tataatacca gcaccttccc gcaatctgat cgcgaaatgg gtaataaatc ctatggtctg
     5461 gcatgggata tggatacgca actcgcatgg gccaaactac gtaccaccgt tggttgggat
     5521 catattagtg attatacccg tcacgatcat gacatctggt acaccgaact ttcatgtaca
     5581 tatggtgata ttacagggcg ttgtacccgt ggcggattag gacacatttc ccaggctgta
     5641 gataattaca ccttcaaaac acgcctggac tggcaaaaat tcgccgtggg tgatgtttcg
     5701 catcaaccct acttcggcgc ggaatacatc tattccgatg catggactga acgccataac
     5761 cagtctgaat cctatgtgat taatgctgca ggaaagaaaa ctaaccatac catttaccat
     5821 aaaggtaaag gcagcctggg aattgacaac tacacgctgt atatggcgga tcacattagc
     5881 tggcggaatg tgtcgttaat gcccggtgtg cgttatgact atgacaacta tctgtcaaac
     5941 cacaatatct ccccgcgctt tatgacggaa tgggatattt ttgctgatca aacctcaatg
     6001 attaccgccg gttataaccg ttactatggc gggaatattc ttgatatggg attacgtgat
     6061 atccgcaata gctggacgga atcggtatca ggtaataaaa ccctgacgcg ttatcagaat
     6121 ttgaaaacgc cttataacga tgaactggca atgggattgc agcaaaaaat cgataagaac
     6181 gttattgcac gcgcaaacta tgtttaccgt gaagcgcatg atcaaatcag caaaagcagt
     6241 cgtaccgaca gcgcgactaa aaccaccatt actgaatata acaacgatgg caaaaccaaa
     6301 acgcattcgt ttaacctcag ttttgaactg gccgaacccc tgcatatccg ccaggtagat
     6361 attaacccgc aaattgtctt tagctatatc aagagcaagg gcaacttgtc gttaaacaat
     6421 ggttatgagg agagcaatac cggtgataac caggtggttt ataacggtaa tctggtctct
     6481 tacgatagcg ttccagtggc agattttaat accccattaa agatctcctt aaacatggat
     6541 ttcacgcatc aaccgagcgg gttagtgtgg gcgaatacgc tggcctggca agaagcgcgt
     6601 aaagctcgca ttatccttgg taagacaaat gcgcaataca tcagcgaata ttcagattac
     6661 aagcagtatg ttgacgaaaa actggatagc agcctgacct gggacacccg cttgtcctgg
     6721 acgccacaat ttctgaaaca acaaaacctg acgatcagtg ccgatattct caatgtactg
     6781 gatagcaaaa ccgctgttga tacaacgaac accggtgtgg cgacctacgc cagtggccgt
     6841 actttctggc ttgatgtcag catgaaattt taatagttcg gattttactt aaccgggtaa
     6901 attcgcccgg ttttcgcatg gagataatta tgagaaacct ctgtttctta ctgacgttag
     6961 tggcaactct gttgctcccc gggcggctga ttgccgccgc cttacagcag gatgaaaagt
     7021 taattaccgg gcaactggac aatggcttgc gatatatgat ttatccgcat gctcatccaa
     7081 aggatcaggt aaatttatgg ctgcaaattc ataccggttc attgcaggaa gaagacaatg
     7141 agcgcggcgt ggctcatttt gtagaacata tgatgtttaa cggcgcaaaa acatggccgg
     7201 gtaataaagt catcgaaaca tttgagtcaa tgggcctgcg ttttggtctc gatgttaatg
     7261 cctataccag ctatgacgaa acggtgtatc aggtgagttt gccgactacg cagaaacaaa
     7321 atctgcaaca agtgatggca atcttcagtg aatggagtaa tgccgcaacc tttgaaaaac
     7381 tcgaagtaga cgctgaacgt ggcgtaattt ctgaggaatg gcgtgcccat caggatgcga
     7441 aatggcgcac ctctcaggcg cgccgccctt tcctgctggc taatacccgt aatttagacc
     7501 gtgaacctat cggcctgatg gatactgtcg ccacggtcac accggcacaa ttgcgccaat
     7561 tttatcaacg ctggtatcaa ccaaataata tgacctttat cgtggtcggc gatatcgaca
     7621 gtaaagaagc gctggcgctg ataaaggata atttaagtaa gtttccggct aacaaagcag
     7681 ctgaaaatcg cgtctggccg acaaaagccg aaaaccacct gcgctttaat atcattaatg
     7741 ataaagaaaa ccgggtgaac ggcatcgcac tctattatcg cctgccaatg gtacaagtga
     7801 acgatgagca aagctttgtc gaacaagctg aatggagcat gttagttcag ctgttcaatc
     7861 aacgtctgca ggaacgcata cagtcgggcg agttgaagac tatttctggc ggcactgcgc
     7921 gcagcgttaa aattgcaccc gattatcagt cgctgttttt ccgtgtaaat gcacgagacg
     7981 ataatatgca ggatgctgcg aatgcattaa tggcagagtt ggcaaccatt gatcagcatg
     8041 gtttttctgc tgaagaactc gatgatgtca aatctacccg cctcacctgg ctgaaaaatg
     8101 cggttgatca gcaagctgaa cgtgatttac gtatgctgac cagtcgcctg gcatccagct
     8161 cattaaataa tacgccgttc ttgtcgccgg aagagacata tcaactttcg aaacgtctgt
     8221 ggcagcaaat taccgtgcaa agtctggcgg aaaaatggca gcagttaaga aagaaccagg
     8281 acgcattttg ggagcaaatg gtaaacaatg agcttgccgc caaaaaagca ttgtctcctg
     8341 cggctatcct ggcgctggaa aaagagtacg ccaacaaaaa gctggcggct tacatcttcc
     8401 caggcagaaa tttatcgtta acagtagacg ctgacccaca ggcggaaatt agcagcaaag
     8461 aaacgctggc tgagaatctg acatcattaa cactttccaa tggtgccaag gttattctgg
     8521 caaaatccgc gggtgaagag caaaagctac aaattattgc cgtatcgaat aaaggcgatt
     8581 taagtttccc tgcgcagcaa aaatcactta tcgcgctggc aaataaagca gttagcggaa
     8641 gcggcgttgg cgaactctcc tcttccagcc tgaaacgctg gagtgcggaa aactcggtaa
     8701 ccatgagcag taaagtcagt ggcatgaata cgttgctctc cgttagcgcg cggactaata
     8761 accctgaacc tggtttcctg ttgattaacc agcgaatcac ccacagcacg attaacgaca
     8821 atatttgggc atcgctacaa aatgctcaaa ttcaggcgtt gaaaacgctc gaccagcgtc
     8881 cagcggagaa attcgcccag ctgatgtatg agacgcgcta tgctgatgac cgcacgaaat
     8941 tactgcaaga aaatcagatt gcacagttta ctgccgcaga tgcgctggct gccgatcgcc
     9001 agttgttttc atctccagcg gatatcacgt ttgtcattgt cggtaatgtc gcagaagaca
     9061 aactcgtggc gttaattacg cgttacttag gctcaatcaa acactctgat tcgccattag
     9121 ccgcaggtaa accattaact cgcgcgacgg acaacgcatc ggttactgta aaagaacaaa
     9181 atgaacctgt ggcacaggtt tcacagtgga agcgttatga ttcccggaca cctgttaatc
     9241 tggcgacgcg tatggcgctc gatgctttta acgtcgcact ggcaaaagat ctacgcgtta
     9301 atattcgtga acaggcatct ggggcataca gcgtttcttc tcgcctctcg gttgatcctc
     9361 aggctaaaga tatcagtcat ttgctggctt ttacttgtca accagaacga catgatgaac
     9421 agttaacgtt agcgaatgaa gtgatggtta agcgtttgac taaggggatc agtgagcaag
     9481 aactgaacga ataccagcaa aacgttcagc gcagcctcga tatccaacag cgtagcgttc
     9541 aacaattagc gaacactatt ataaatagtc ttattcaata tgacgatcct gcagcatgga
     9601 ctgagcagga gcaattgttg aaacaaatga cgatagagaa tgttaacact gccgttaaac
     9661 aatatctttc tcatccggta aatacttata ccggagtatt attgccaaaa taataacagc
     9721 cccgtcaaca catcgttggc ggggatttta gcaatattcg ctatttttat gtaataattt
     9781 tataaatgcg ttcaaaataa taaccaagta ctaatagtga tattttaatg tctgattttt
     9841 acgtgataat tcaggaggca cagaaagcgc ataaaaataa caacataaaa caccttgcca
     9901 ccacccaaaa attctctatt gcattgtttt taatgaaaaa aatatcattc caacaatatc
     9961 tcacgataaa taacattagg attttgttat ttaaacacga gtcctttgca cttgcttact
    10021 ttatcgataa atcctacttt tttaatgcga tccaatcatt ttaaggagtt taaaatggat
    10081 aagaagcaag taacggattt aaggtcggaa ctactcgatt cacgttttgg tgcgaagtct
    10141 atttccacta tcgcagaatc aaaacgtttt ccgctgcacg aaatgcgcga cgatgtcgca
    10201 tttcagatta tcaatgatga attatatctt gatggcaacg ctcgtcagaa cctggccact
    10261 ttctgccaga cctgggacga cgaaaacgtc cacaagttga tggatttatc cattaacaaa
    10321 aactggatcg acaaagaaga atatccgcaa tccgcagcca tcgacctgcg ttgcgtaaac
    10381 atggttgccg atctgtggca tgcgcctgcg ccgaaaaatg gtcaggccgt tggcaccaac
    10441 accattggtt cttccgaggc ctgtatgctc ggcgggatgg cgatgaaatg gcgttggcgc
    10501 aagcgtatgg aagctgcagg caaaccaacg gataaaccaa acctggtgtg cggtccggtg
    10561 caaatctgct ggcataaatt cgcccgctac tgggatgtgg agctgcgtga gatccctatg
    10621 cgccccggtc agttgtttat ggacccgaaa cgcatgattg aagcctgcga cgaaaatacc
    10681 atcggcgtgg tgccgacttt cggcgtgacc tacaccggta actatgagtt cccgcaaccg
    10741 ctgcacgatg cactggataa attccaggcc gacaccggta tcgacatcga catgcacatc
    10801 gacgccgcca gcggtggctt tctggcaccg ttcgtcgccc cggatatcgt ctgggacttc
    10861 cgcctgccgc gtgtgaaatc gatcagtgct tcaggccata aattcggtct ggctccgctg
    10921 ggctgcggct gggttatctg gcgtgacgaa gaagcgctgc cgcaggaact ggtgttcaac
    10981 gttgactacc tcggtggtca gattggtact tttgccatca acttctcccg cccggcgggt
    11041 caggtgattg cacagtacta tgaattcctg cgccttggtc gtgaaggcta taccaaagta
    11101 cagaatgctt cctaccaggt cgctgcctat ctggcggatg agatcgccaa actggggccg
    11161 tatgagttca tctgtac
//
LOCUS       DADMGC010000128        11016 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.128, whole genome shotgun sequence.
ACCESSION   DADMGC010000128 DADMGC010000000
VERSION     DADMGC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.128"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..212
                     /gene="yfdR"
                     /locus_tag="JNZ73_003351"
     CDS             <1..212
                     /gene="yfdR"
                     /locus_tag="JNZ73_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY8733519.1"
                     /translation="VMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVI
                     PLAPGHAYGMFMERFNELSELRKCA"
     gene            203..565
                     /locus_tag="JNZ73_003352"
     CDS             203..565
                     /locus_tag="JNZ73_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY8733520.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     QENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            565..870
                     /locus_tag="JNZ73_003353"
     CDS             565..870
                     /locus_tag="JNZ73_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733521.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGNNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            870..1220
                     /locus_tag="JNZ73_003354"
     CDS             870..1220
                     /locus_tag="JNZ73_003354"
                     /inference="COORDINATES: protein motif:HMM:NF024139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY8733522.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTRQACIAALQSPLHTVQVSAGDGITEERKCHSSAEVKYGTPVSHCRTVKDLNSLL
                     EQRTKGRRQNSMTS"
     gene            1097..2260
                     /locus_tag="JNZ73_003355"
     CDS             1097..2260
                     /locus_tag="JNZ73_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY8733523.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAEACRKKGKPVPEYTPKPASVVTKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNKESTNDV"
     gene            2610..2972
                     /locus_tag="JNZ73_003356"
     CDS             2610..2972
                     /locus_tag="JNZ73_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY8733524.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            2969..3886
                     /locus_tag="JNZ73_003357"
     CDS             2969..3886
                     /locus_tag="JNZ73_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY8733525.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            3899..5164
                     /locus_tag="JNZ73_003358"
     CDS             3899..5164
                     /locus_tag="JNZ73_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HAY8733526.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(5231..5854)
                     /locus_tag="JNZ73_003359"
     CDS             complement(5231..5854)
                     /locus_tag="JNZ73_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8733527.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSERY
                     THTNIQVTSSGGGKVIVLLNGTTFQSAGDYQPSADRPYWITGNNCELIDIGCTFMEIT
                     SKATSVSAESVTRSGRINANGSVDIAPGVSSVNAHATGVYDVTFSHPLAAATNGYVVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(6596..6943)
                     /locus_tag="JNZ73_003360"
                     /pseudo
     CDS             complement(6596..6943)
                     /locus_tag="JNZ73_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569055.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
     gene            7065..7325
                     /locus_tag="JNZ73_003361"
     CDS             7065..7325
                     /locus_tag="JNZ73_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HAY8733528.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQCAKMNNSSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSN"
     gene            complement(7343..10078)
                     /locus_tag="JNZ73_003362"
                     /pseudo
     CDS             complement(7343..10078)
                     /locus_tag="JNZ73_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(10079..>11016)
                     /locus_tag="JNZ73_003363"
     CDS             complement(10079..>11016)
                     /locus_tag="JNZ73_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8733529.1"
                     /translation="SSPEAMQSWLQNNAGELARVGVNPQDVAQMYQQNPRQFGEFVDH
                     LGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAITMRGQDIQANLGQQRIN
                     LDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQLQQKQQALNDGYKDGIN
                     TLTTSMLTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQARLDTFKSQAYLTAVQAM
                     RGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLNQKRNEAVGKYVQQNGI
                     KRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
BASE COUNT         2987 a         2490 c         2440 g         3099 t
ORIGIN      
        1 cagttatgag tacgcccgtg aaatatgccg atctcatcat gctggcaacc gaacgccgcg
       61 atctcgggct tgatgatggc tctttctggc ctgtactgga aggtatcccg gcaacagaga
      121 tgttcaacgt gattccactg gcaccgggcc atgcctacgg gatgtttatg gaacgcttta
      181 acgagttatc ggagttacgc aaatgcgcat gaatgttttc gaaatggaag ggtttcttcg
      241 tgggagatgt gtaccgcgag atctgaaagt aaatgaaaca gatgctgaat acctggtgcg
      301 taaattcgat gcgcttgaag ctaaatgtgc agcacaggaa aacaaagtaa taccagtgtc
      361 aactgaactg ccaccagcaa atgaaagtgt tttgttattc gatgctaacg gagaaggctg
      421 gctaattggt tggcgttctc tctggtacac ctggggacaa aaagaaaccg gagaatggca
      481 gtggacattt caggtcgggg accttgaaaa cgtcaatatc actcactggg cagtaatgcc
      541 aaaagcaccg gaggctggag cataatgacc acttttaccg acaaagaact gattaaagaa
      601 attaaagagc gtatcagcag ccttgacgtg cgagacgata ttgagcgccg tgcttatgaa
      661 atcgcactcc tatctctgga agtagaacca gatgaacgcg aagcttatga attattcatg
      721 gaaaagcgtt tcggtgactt agtagatcgt cggagagcaa aaaacggcaa taacgaatac
      781 atggcatggg atatgactct cggttggatc gtctggcagc aacgagctgg tatccatttt
      841 tcaacaatgt cacagcaaga ggtgaaataa tggagccata cagcctcaca ctcgatgagg
      901 cctgtcattt tctcaagata tccagaccga ctgccattaa ctggatacgc acagggcgtc
      961 ttcaggcaac acgcaaagat cccactaaga ataaatctcc ttacctcaca acacgacaag
     1021 cctgcattgc ggctcttcag tctccgctgc atactgtcca ggtgagcgcg ggtgatggca
     1081 taacagagga aagaaaatgt cactcttccg cagaggtgaa atatggtacg ccagtttcac
     1141 attgccgaac ggtaaaagat ttaaacagtc tcttggaaca aaggacaaaa ggcaggcgac
     1201 agaactccat gacaagctaa aggctgaagc atggcgggtc agcaaacttg gtgaaatacc
     1261 tgatataacg ttcgaggaag cgtgtgtcag gtggcttgaa gagaaagcac ataaaaaatc
     1321 actggacgat gacaaaagcc ggatcggatt ctggcttcaa catttcgcag gaatgcaact
     1381 aagagacatt actgaatcaa aaatttattc agcaatgcag aaaatgacga accggcgtca
     1441 tgaggaaaac tggaaactca gggcagaagc atgcagaaaa aaagggaaac ctgttccaga
     1501 atacacgcca aaaccagcgt ccgttgtaac gaaggctacg catctttcat ttataaaggc
     1561 cctactaaga gccgcagagc gtgaatggaa aatgctggat aaggcaccaa ttattaaagt
     1621 gcctcaacca aagaataaac ggatccgctg gctggagccc catgaagcac aaaggctgat
     1681 tgatgaatgt ccggagccat taaagtctgt tgttgaattt gcactggcaa caggcttaag
     1741 acgctcgaac atcatcaacc ttgaatggca acaaatagat atgcagcgcc gggtggcatg
     1801 gataaacccg gaagagagta aatcaaaccg cgcaatcggc gttgcgctga atgatactgc
     1861 atgtcgcgtt ttgaaaaaac aaatcggaaa tcatcaccgt tgggtatttg tgtacaagga
     1921 aagctgtacc aaaccagacg gaacgaaagc gccaacagta aggaagatgc ggtatgacgc
     1981 aaacacagcc tggaaagcgg cgctgagacg ggctggtatt gatgatttca gatttcacga
     2041 cttgagacac acctgggcaa gttggctggt tcaagccgga gtcccgttgt cagtgttaca
     2101 ggaaatggga ggctgggagt ctatcgaaat ggttcgtcga tatgctcacc ttgcacctaa
     2161 tcaccttacc gaacacgcac ggcaaataga ctcgatcctg aacccatcgg tcccaaattt
     2221 gtcccagtca aaaaataagg aaagtactaa tgatgtgtaa cttattgatt taaatggtgc
     2281 cgataatagg agtcgaacct acgaccttcg cattacgaat ctgtagcacc aatcataact
     2341 atctgtttta gcaagcatta accgcattca ctaagatata gttgatggca caaacagaaa
     2401 gttgatgaat gatgttgcca tatatatgtc acaaatacgg cacaacgatc ttcaaacata
     2461 tagccacaca ttcacagaag agcacaaagc cttgcaatcc agtgcaaaga tttgtgtgcc
     2521 agagtttttc tcatcaacta ccgcaagtat cgatcgattg agacttggat gatagacttc
     2581 atgcctttga ttattagctg atagaagaaa tgttaaagct atttgcaaag tacacatcta
     2641 taggcgtgct gaacacactt atacactggg tggtttttgg tgtatgtatc tatgccgcgc
     2701 ataccaatca ggctatggca aacttcgcag gtttcgttgt ggctgtgagt tttagcttct
     2761 ttgcgaatgc aaaattcaca ttcaaagcat caactacaac gatgcgctac atgttatatg
     2821 tcgggttcat gggaacactg agtgctactg ttggatgggt tgctgataga tgttcacttc
     2881 ctccaattgt cactcttgtt accttctccg ccatcagctt ggtgtgcggt ttcgtctatt
     2941 caaagttcat tgtctttagg gatgcgaaat gaagatatct cttgtcgttc ctgtcttcaa
     3001 tgaagaagaa gcgataccaa ttttttataa aacggtacgt gaattcgaag agttgaagcc
     3061 atatgaagta gaaattgttt tcataaatga cggaagcaaa gacgctacgg agtcaatcat
     3121 taacgctctg gctgtttctg atccgctagt tgttccgctg tcatttacac gcaactttgg
     3181 taaagaacca gcattgtttg cagggttaga ccatacaacc ggcgatgccg tgattccaat
     3241 tgatgttgac ctgcaagacc cgattgaggt tattcctcat cttattgaaa agtggcaggc
     3301 aggtgctgac atggtgcttg ctaaacgttc agaccgctca actgatggac gactgaaacg
     3361 taagacagct gagtggttct ataaattaca caacaaaata agcaccccaa aaatagagga
     3421 aaatgtcgga gattttcgac tcatgtctcg tgaggttgtg gagaacatta aactattgcc
     3481 tgagcgcaat cttttcatga aaggcatact gagctgggtt ggtggtcaga cagatgtcgt
     3541 tgaatatgta cgcacagagc gtgttgctgg catctcaaaa tttaatggct ggaaattatg
     3601 gaatctggca ctggaaggta tcacaagttt ttcaaccttt cctcttcgcg tatggactta
     3661 tataggcttg tttgttgcaa gcatttcatt tttatatggt gcatggatga ttatagacac
     3721 cattgtcttt ggtaacccag tacgcgggta tccctccatg cttgtatcaa tacttttcct
     3781 gggtggagtg caactgatcg ggattggtgt tctcggtgaa tatataggta gaatttattt
     3841 agaaacaaaa tcaagaccac ggtacttaat taaaagcaga aaataacagg atgatcttat
     3901 gaagatattc ataatgcgca attggcacaa gatatcaatt tttatacttg catttacttt
     3961 gatttggctt aggcgcattg acattttgac aaatgcccag ttttgggctg aggatgcagt
     4021 tttttggtat aaagatgcat acgaacaagg ttttttcagt tcactcataa ccccaagaaa
     4081 tggttatttt caaacagtat caacactcat tgttggtgcg acgacattta ttaatccaat
     4141 ttacgcacca ttattatcaa acttttttgg aataataatt agagctataa ttatatggtt
     4201 cttatttacc gatagattta aattcctaag caccacatca aaaatcttca tctctgtata
     4261 tttaatttgc atgcccgggc ttgatgaagt ccaagccaat ataacaaatg ctcactggta
     4321 tttatcattg tatgttgcaa tgataataat atctgaagaa tccaagtcaa aattatggaa
     4381 agcacatgat ttatttttta tagtattatc tggattgagc ggtccattta ttatattcat
     4441 aattgcatca tctgttttta aatatttaca cttatcaaaa ggagtaataa gcataagagg
     4501 tttatactta ttttatactc gactgccata tttggcaatg atcttgtgtg ggctgataca
     4561 ggcaacatca ataattctta catttaatgg aacaagatca catgctccat taggattcag
     4621 ttttgatgtt atgtcatcta taatatcatc taatgtattt cttttttcat ttgtcccttg
     4681 gaatatcgct aatgctggat gggataatca ccttttatcg tacacactat caactttagt
     4741 aattacatta tgtgttttca tttatattaa aggaaattgg caaatgaaag tgtttgccac
     4801 ccttccaata cttattgtcg cattttctat ggcaaaacct caactggcag attctgttcc
     4861 tcaactgcca acacttgcaa ccggaaacgg gtcaaggtat tttgtaaata ttcatattgc
     4921 aattttttca ttaatatgcg tttgtttgtt tcaatgcata aagaataaaa ccttaaaaat
     4981 attctttaaa atctatgttt cagtcatcct tattgcaatg ataaaattaa acttttttat
     5041 aacccctctt cctgacatga actggtctca aggagcagaa ttaataaata aagcaaaaca
     5101 tggagaagcc gtctcattta cagtgctccc tccatggtta actcttgatt taataaaaaa
     5161 ataaaacttc acattaataa ataacaatga atccgcctcc ctacgtattc ccgtagggag
     5221 gctgtcgtgt ttatagtagc ctggtaatgc taaatgcaaa cgaactggag attcctgctc
     5281 cgctaagcgt attgcgcagg gtcaccctaa atgtatctac accaatataa gtaacatcac
     5341 aactaactga atcaggcgca gatataggtg ttatctgaac cacataacca ttagtagctg
     5401 ctgcgagcgg atgagaaaac gttacatcat acacaccagt agcatgcgcg ttaacggatg
     5461 aaacacccgg cgcaatgtct acactaccat tggcgttaat tctccctgag cgcgtaactg
     5521 actctgcaga aaccgatgtg gccttactgg ttatttccat aaaggtgcaa ccaatatcga
     5581 tcaattcgca attgttccct gttatccagt atggtcgatc tgcacttggc tgataatccc
     5641 ctgcgctctg gaacgttgtg ccattgagta agactataac ttttccccca cctgagcttg
     5701 ttacttgtat attcgtgtgc gtgtagcgtt cactgctcac gcgatgaaaa tttccaccgt
     5761 ttataactac cgttacaggt cgcgtcccag tattatctat agcaaggtct gcccccccgg
     5821 cattagcctc gaagtatggg ttgtttaaca ccaaagcaca cgaaccgtta aggccattta
     5881 tggctaattg cgcaccacct gtaccggaat ccccctgagt accatttcct tcacaagtca
     5941 ggctgttgat agtagttcct gccccccatc tatcaccaag tataccccat cgtgtattct
     6001 ggcttgcagt taatcggtta actgtaatgg cattaagctc tgacccttca ctggagttaa
     6061 gtgcgcgcca gccaatacgg ttaccctgta agtacacatt atctaactct gacgttaaca
     6121 ccgagtctgt acgcaaccct tcgccggtag tgtgcccata agacacaatg ttctctaatt
     6181 tagtgtatgc tttattggtt acgagaagac cgtaagacga tcccgaacct ttaagtgtaa
     6241 tgtcccccag ataatcatag gcgtgtacac gttgggttgc cccgactgta tcaccgagca
     6301 tctttaagtt gaaatccgag gcatggcagt taaatatcgt ttctgccata ttttctccgg
     6361 aataatgagc acggactcct gtcgaaccaa taaatttagc gtcatagttg ccgtaatcaa
     6421 gaacgagtgg tgacgcgata tcgtacaccc cttgctttat ttttaatgtt ccaccgaaag
     6481 gccacgatgt aagcgggtct tgatatattt cattttttat tgcaaatagc tcctcttgga
     6541 gatttttgcc gctattagtg ccaatgtgag cagctccttc atctgattga agtaactgct
     6601 ttaactgatc aggatcgtac ttcagcacat tctgaaaata gaactgctgc gaaccatacg
     6661 catcatatac agccattgaa tggccttgct cagttacaaa tttggcaatc tgcccgttgt
     6721 acacaggata tccggcagca ttgataacaa ttggctgaga aacagggacg tgagatccgt
     6781 cttcattctc tacataaacc tgaatctggt tttcaggatt taccgggtca gtgtcaattt
     6841 ttccgatata aattttgcca ttggctacgg ctttaaaaga acgcgccata gtgaagagtt
     6901 gcgaaggcat gctgaccaca acatttgcgg cgatatctga catttcattg ctccagacga
     6961 atgatatgat gcaatcatga tgtgattgca taccgaaatg gcactattga atatttatcc
     7021 agtaggttac gatgccattc cacccaactg gtgaggcatc aaggatgtac agcaaatacg
     7081 acgaggcaca gtttcacttg agacttccgc atgaactcca cgcgaaaatt aaacagtgtg
     7141 cgaagatgaa taacagttcg ctgaactcag agataattgc agcgattgaa gaatcattgg
     7201 ctaaacaaag ctctgcatca gtttacattg acgatgcaga gcgtatggca gaacaacaat
     7261 ctgatatggt taagaaaatt gtctttgata cgctcaagga gctatataaa aaagacagca
     7321 actaaccatc cgtggctttc cgttacttct ggttatcatc actgttcatc cagtacatga
     7381 tgcctagcct tgatatggtt ttcttatctg attctggaag tgagttgtaa aacgagcgcc
     7441 aagcagaaga gcgtcttaca gttgtatcaa cgatgctttc tggtgcttgt tttactgcca
     7501 gccttgctgc tttctggaat tctggtgagc taattagctt ttctgcagat tcaattgatt
     7561 cagcgccgcc catcgccctg attttgctgg cgacttttgc cccaatttct gacccagcaa
     7621 cagcacctac tgcactgaac ggtcctgaca cagttgaacc aaccgcagtg ccaatgcgtt
     7681 gggcatggtt agcaacaaat tcatttgccg cagtgacgcg attaaaccgt ttgacgaact
     7741 cgtttagtct tcctgtagtt atctcgtaag attttgcatc tttgatagcc tttgcgacct
     7801 tatatacatc gttcagacct gacatagtct cttttggtaa atgtctggca agattgcgca
     7861 tctgcccgtt tgctaacatg ttttgatacc agtcagcaaa ccctgcagga ttaaaatcag
     7921 cgccacgttt tccgttcgaa agcatatccc gaagacctgt tcccagtagc tcggttctta
     7981 gcttcctgga cggcgtgttt tgcatcaatt cacgaaatcc tttggcatcg cctttcgaca
     8041 ttgcctgtag tgctgtagtt gctttgcgag aaacatcacc gttcagcgtt ctgccagtta
     8101 gattaatcat ttgctcttcc atgctttttc gcatctgaac aagcctctga gcaacttcaa
     8161 aatcacgtaa tgcatttgtc tctgacagcg ccgccttttg atcatcggcg agcgaaccgt
     8221 aaagcctcga taaagcagcg cgatcagcat ctttatatgg tccagaattc ttatgaagtg
     8281 catcaccaac aagtcgtcgt tgcttattga gatttgcata agttaatgcg ccgttcttgc
     8341 ctggattaac tgcccgaaag actctctttt caatagggtc aaggtttttc atcccgccaa
     8401 gatcttctgc cacctgtttg agcattgacc ttgttgatgg cgcatcaatt tttgcacctg
     8461 caggcatcgc cttttcaaca ttgcgataaa gctggtcgct tcgtttcatc agcgcagaca
     8521 ttcttccatt gaccgtatca ataaacttgt cgctcatacc aagagcatct tttgcaccgg
     8581 aaacgcgatc aattatctca cccgcgccct gtgctagctg cctgattgct tcattttcct
     8641 gcacctgtag agcagaaccc gcacgggact tgattgcttg ctcaactgcc ttgtattgct
     8701 cgttcccgga aaagtgtgaa gggagtaatg aatcaacatt aagcctttca gcagactcca
     8761 gaacttcagc ctgaggattg atatctagtt catcaagcga tgaagcgaga tttggccttt
     8821 tttgtgctgc tactgtgcga acgacttcct ctgggttctt cgtcgcaggc gtcattggta
     8881 caccttgtgc tactcttgcc tctgcggcat gaaccgcagg atttgttgct gcagcggcat
     8941 caccggagaa tggtgatgtt tctgttggcg cagtggtcgc tctctgtgca gatgttttat
     9001 tgccactaat tatatttgat gcaccacgaa caccacgggt aacaccattt atcaggccat
     9061 gcgtagcaat gctaagtccg gcattaatag cggcattttc ggcaaaatca ccccgttgat
     9121 tcgccgcgtc agcgagagaa ccaatgacca tgtttccagc tacacctgct ccaggaacaa
     9181 gataaccgcc tattgactct ccagcttgcg cgtaggggtc tgtcggtctg tctactggac
     9241 gataaacatc atccaatact tttggccccc caagcccctg actgattgca ttaatcagac
     9301 ttgcgccacc ctgcaatacg tcaaatggta tgtttaccag accacgacca gcctgttctg
     9361 caatttaccc tgcactttga ccaccagtga gccaatcgcc agcttgttgc atcaatgatg
     9421 gttcttcccg tgttggtgca ttattggcct gattaactgt ttgttgctga acaggtttcg
     9481 gtgcgacaga acttacgggt tgaggtggct gctgactggc tgcctgctgc tcaatctgag
     9541 caaatggatt atttgggtct gactgcacac ctgatgccga tacttgttcg gatgactgta
     9601 cttccagttg tgcaaacggg ttgttagggt cttgctgagg atgtaccttt gcagaggtgg
     9661 cgcgctgttc gactgttgag tctgtcaccg ggtcaccagc ccattgagca aagcgatcat
     9721 caacgtaacc gcggccttca ggtcctggcg tatattcacc acgctttgcc ttcataacgt
     9781 tgccgggacc gtcgtgataa gcctgaagag cgtcacgcca gttaccaaac tgctggtata
     9841 tctttgccag atagcgcgcg ccagcgtcag cctgatattc ggggttttgc atttgctcat
     9901 cggtataacc catatcacgc catgtcccag gcatgacctg agtcaatcct acagccccgg
     9961 cggagcttac tgcggcaggg ttgtaagaag actccttggc acccagtgca gtcatcaacc
    10021 cttctggcac accgtaacgt gcgccagcct gctctaacaa atcacggtaa ttagccattt
    10081 actgccccaa agatggaaga tatccgtagc gattaatgaa gtcgattgac agctcggggt
    10141 gctgcttcag gtaatctata gaagcctgag gcgcttccac tcgcttgata ccgttttgct
    10201 gaacgtactt accaaccgcc tcattacgct tctggttgag cgtgttcagg atgacgccag
    10261 cgttgcgacg aaaggactcc tcgctctgcg agttctgcag cgaaccaaca gcctggtcga
    10321 gctttttgcc ctcggcatca gaaagtgcgc ccatgcctcg catggcctga accgctgtca
    10381 ggtatgcttg ggatttaaag gtatcaagtc gtgcctgagt gtctgcagcc tgtgagcctg
    10441 gaacgttggg gattactcca cgtaagcctg taatgctctt aagtgaagga gaactaacga
    10501 tatcgttcag agttaacatg ctggttgtga gggtgttgat gccgtctttg tagccatcat
    10561 ttagcgcttg ctgcttctgc tgcaactgct ggttgttggc tgctatgcgg ctctgtattt
    10621 cctggcgctt caggtcgtta gtttctgctg atagcatccg gtcaaggcgc ttattttcgt
    10681 tgttaatgcg gtttgtttct gcgtccagat taatgcgctg ctgacctaaa ttcgcctgga
    10741 tatcttgccc gcgcattgtg attgcctgat tccgagcggc ggtttgcgaa tccaggtcct
    10801 gaccgcgcat ggtaacctgg cgaccctgca ttttatcctg taggtcaaag tatttttcgg
    10861 gtccgagact gttcatcccc aggtgatcga caaattcgcc gaactgccgc gggttctgtt
    10921 ggtacatctg agcgacgtcc tgaggattaa cgccaacacg agctaactca ccggcgttgt
    10981 tttgcagcca tgattgcatt gcttctggag acgatg
//
LOCUS       DADMGC010000129        10983 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.129, whole genome shotgun sequence.
ACCESSION   DADMGC010000129 DADMGC010000000
VERSION     DADMGC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.129"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="JNZ73_003364"
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="JNZ73_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HAY8733530.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(998..2020)
                     /locus_tag="JNZ73_003365"
     CDS             complement(998..2020)
                     /locus_tag="JNZ73_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8733531.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADICGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNLVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /locus_tag="JNZ73_003366"
     CDS             complement(2034..3536)
                     /locus_tag="JNZ73_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY8733532.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDMREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLNTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4633)
                     /gene="ytfQ"
                     /locus_tag="JNZ73_003367"
                     /pseudo
     CDS             complement(3676..4633)
                     /gene="ytfQ"
                     /locus_tag="JNZ73_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4943..5473
                     /gene="ppa"
                     /locus_tag="JNZ73_003368"
     CDS             4943..5473
                     /gene="ppa"
                     /locus_tag="JNZ73_003368"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HAY8733533.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5853..6194)
                     /locus_tag="JNZ73_003369"
     CDS             complement(5853..6194)
                     /locus_tag="JNZ73_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY8733534.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6197..9976)
                     /gene="tamB"
                     /locus_tag="JNZ73_003370"
     CDS             complement(6197..9976)
                     /gene="tamB"
                     /locus_tag="JNZ73_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HAY8733535.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGIYTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9973..>10983)
                     /gene="tamA"
                     /locus_tag="JNZ73_003371"
     CDS             complement(9973..>10983)
                     /gene="tamA"
                     /locus_tag="JNZ73_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HAY8733536.1"
                     /translation="APQFDKARETKVLPLTGVVSPRTENTIETGGGYSTDVGPRVKAT
                     WKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGFKRTDLNDTE
                     SDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMISRTRSRGGL
                     MPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGTLGWIETGDF
                     DKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQYNVTGKWWGA
                     VFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHGLQFYIGLGP
                     EL"
BASE COUNT         2519 a         3123 c         2783 g         2558 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccacc aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacgat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac caccaggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcaa atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcaaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccacttct
     3001 tgggtgttga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 atgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgccgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct taacggtcaa
     4141 tggcgacgct ggccccaacg gtgccctgca gctccaccac gttgcatggt ttgccattca
     4201 cttcttttac cagccagtca ccgatcaact taccttcgag gatgttgtcg gcagtaacgg
     4261 tggtcatata gagagatttg tctttcacat cgatggaacg gtcgagcaag aagaccggga
     4321 tttcggcatc tttcgcctct tttaataccg gttcccaacc cgtcgcaacc actggagcga
     4381 taaagatcgc atccacccct tgtgcaacga aggaacgtac cgctttaatc tggttttcct
     4441 gcttttgctg accatcggca attttcaacg taattccgcg tttttcggcc tcacttttcg
     4501 ccacattggt ttctgcggcg cgccagccgg attccgatct gacctgcgaa aatcctacgg
     4561 ttaatggagc ggccaacgcc atagacgaca tggctgccga gactgcagag actacaagta
     4621 agcgtttcca catagggttg tcctcgtcgg ggtaattatt attggtgaaa agatgttcgc
     4681 gaaaaaacta tagacaattc gttatgtaac ggattgcgtt acatcacact tcaaaaaagt
     4741 aaataaaacg ttaatcacaa gtttgtaatc gctttcatct cactatgaaa aatgcggcta
     4801 cggttatgga ttttcctgct ctgtataccg tcttaaaact ggcgaaaaag gaaaatgaag
     4861 acgaaaacaa gcaaagacat tcggcgcgag ttggctataa tacttggcac ttgtttgcca
     4921 catattttta aaggaaacag acatgagctt actcaacgtc cctgcgggta aagatctgcc
     4981 ggaagacatc tacgttgtta ttgagatccc ggctaacgca gatccgatca aatacgaaat
     5041 cgacaaagag agcggcgcac tgttcgttga ccgcttcatg tccaccgcga tgttctatcc
     5101 gtgcaactac ggttacatca accacaccct gtctctggac ggtgacccgg ttgacgtact
     5161 ggtcccgact ccgtacccgc tgcagcctgg ttctgtgatc cgttgccgtc cggttggcgt
     5221 tctgaaaatg accgacgaag ccggtgaaga tgcgaaactg gttgcggttc cgcacagcaa
     5281 gctgagcaaa gaatacgatc acattaaaga cgtaaacgat ctgcctgaac tgctgaaagc
     5341 gcaaatcgct cacttcttcg agcactacaa agacctcgaa aaaggcaagt gggtgaaagt
     5401 tgaaggttgg gaaaacgcag aagccgctaa agctgaaatc gttgcttcct tcgagcgcgc
     5461 aaagaataaa taagttcttc tagcgcaata accctgaacg ccgggcttcg gttagtaagg
     5521 gtttttttat gcccgcgata aataaactct ctattccacc atcattattc tcagcggttg
     5581 caaggcttga acggtaagaa caagcaaacc cgaccaccat tttgctgttc atagccactt
     5641 gctggaagtt agccgacctc actcatactc accgttcaaa gcgtgccccc gttttacccc
     5701 tgcacatttg tgaacgccag cagagttttt tatgcgattg cagatatgga aagcggctcg
     5761 gagagaaaag caaaagatgg aaaagttaca aaaaaaaatc gcgagataca tcgcataaaa
     5821 caacgccacc cgaaggaggc gtgcttttat tattacttat ccctgtctaa ccagtcgccg
     5881 ctttcaatta gctttaatcc atcgacgggt cgttgataaa cgtacatcca tgcactccca
     5941 tacggcgtct gaatcaactg gcgcgcgtat tcaccgcccc tggtgcgcaa ggcatcaagt
     6001 tcggccagcg tggcgttgtc aatacgataa acttcaccgt gtaccgttcc gttccccgga
     6061 actgcgcctg gatagtggcc caggctatac aactggtagt tatcgatact gaaatcgccc
     6121 agtaactggg cattggtcat ccagtgactg ttgccttgtt tgtggcgtaa actgccgtag
     6181 acaaatattc gcattgctaa aactcgaact gatagagcaa atccagtgcc tggtctacac
     6241 cagacacggc ttccagatat agcttaggca tcaggcgata acgtaacgtg agtgttgcta
     6301 tagagtcaaa tatacccacg ccgtatttca cttgcagacc tggcaataca tagccgctga
     6361 ccactacctg ggaggagtcg cctactccca gggtgtcgag cgctaaattg cttacgccaa
     6421 acgtctcgcc gattttaccc acaatctggc cactttgcgc aacccccaga ccaatcagca
     6481 tcgaggtcat tgccgcactg tcgctctgat cgctctccag cccttgtcca cgtagcaaat
     6541 aagacaatgc agcttgttgc gacatcgccg ggtcagagaa gatctccgct ttcggttcgt
     6601 ccgccagacc agtgacgcga actccggcga ttacatcgtc ttctgtagca tccgggttac
     6661 gaatagcttc aatattaaga tacggttgat ccggcggacc agagaacagt aactcaccct
     6721 tacgcacaat cagatcctga ccataggcat ggaagcgccc ttcagggatg ttgatctgcc
     6781 cgttcaggcc cagcccttgt ttgtcctgta cgacgttaag atcgcccgtc agccgcgctt
     6841 tcaggccaaa ggcgtcaatg cgcacattgt tgccaacgtg gacaatcagg ttactgttaa
     6901 tcggaatcga cgccgttttc ggctcttccg gttgcaggtt atcgttaagc atcaccacat
     6961 cgctggagac gcctactgcg ctttccggca gatcgtgcac cacgatgcgc gcccacggga
     7021 catccacgcg accatcgagg gtaaacaggt ttggtgtagc ctcgaataca acatctggcg
     7081 atacatccat tcgtaccatc ggcggcacgg tgatccgcac tttactgcct ttcgccgtta
     7141 ctcgtgcccg ccagttttca atttggctcc agtcggcatc accgttcagg tagatttcac
     7201 cctgctgggt ccgtactgta ccggcaagcg tcgagcgcat accgttaaag ttgaccgcaa
     7261 gctggctcgg ctgcatatca aacggcataa agttgccgtc gatatccaca cccgtaacct
     7321 gaagctgacc aaacaactgc gggctttgca catcaccacc cagacgcaag ttggcactca
     7381 ccatccccgc tgctttttcc ccacgggtaa agatggggtt tatcatcgca aggttgaagt
     7441 tacggatatt gacgttgcca ccaagattac ggcggccttg cggatcggtc acctgcacct
     7501 gtccatcaaa ctggccgtta ttggtcaggc ggatggtcca gcccaattcg gcacggttgt
     7561 tacgcaattc cgccgtcaga ttcagtgtct gaaacgccac cggcagcgcc gcatcgttga
     7621 cggtttgcgt tacctgcacg ttacgccccg aaagggtgat actgccctgc ggcagcccct
     7681 ctttcgtggt gtcccaggca acatccgctt tacccgtgaa gataccgctg gcctgagtgg
     7741 tttctggcat aaacggtttc agcatggcga ggtcgaagcg gttgagattc accaccgcac
     7801 gcccttcggc ccccgcatcg atagtttgcg gcacgcacag ttccgcattc gggttaagcc
     7861 agcagtgtgg cccgatgctg attttttgct ccttattgcg gtaatccagc gcaatatcgc
     7921 gggtcagcga ccacgggccg accggcgtct ggaagcgggt attgctaaga gttcccttcc
     7981 agcgttcttc tttgcgatca aaacttcctg ccagattaag ctgcccggag acaggctcgc
     8041 cctgaatccg caactgtagc tcgtgctgct tttcgctgcc tttggcattc agggtgacga
     8101 ggttgatatt tacatccggc tgcgaaattt gctcaacgcg tacgtcgagt ttcccggcga
     8161 tctgatcggt ggatttaatg tcgccttcca cgcgaacctg cgccacggaa agttcctgcc
     8221 agcgcaggcc gcgcgcggtg atatctgcca gtagttgtgg cgcttccacc gtgccgcgta
     8281 ctttcaccag ccctttcgct gtaccgccaa gccccggcag cgcgttatcc agccccggcg
     8341 cgttgatggt ggcatcaaga ttgagatctt ttacccccag ctcgcctttc acttcggcac
     8401 tgtttggccc gagttccaga tgaagcccag ggatcatcca ctgcatataa ctgttgcctt
     8461 tcagcgtgcc gtcaacgttc actttgttct gtttgacgtt accggtcagc ttcagttctg
     8521 gcacctccat ctgccaggtg ccgccgtaca ggctaccgcg ggttttaatc aagccattga
     8581 gtttcgacgg ccactccggg aactctttgg cggtgtaaat gccattaagc gttagctcac
     8641 cgcgccaact aatggcctgc tgccagtcga gcaacgcctt gagttcagtt ttcccttcca
     8701 gcgccgcgac ggtgagtttg tcgagattga cctgctgttc attacctttg gcatcaaggg
     8761 taatggtggc tggcgggatc tcctgtccct tcactgccgt acgcatagag agcgtgtaat
     8821 cggtcatttt gccggtaagt ttcagtttca gatcatccgc ctgatactgc ttctcaccag
     8881 tgaacggcca gtaaagctgt ttgctgttca cttccacgtt gagtggcaat ccggcttccg
     8941 ccagtcgcgt ctgggcgcgt aaatccatat ccaccggacc ggaaaggtta acgcctatct
     9001 ccagctgttc gcgcagcgcg ccgcccactt tcagcttcac cttttcacct ttcaacggct
     9061 ccacgttcag tgtgctgttg agggtgatat ccaccggcca gttgtctgac agctgcgccg
     9121 tgccgctggc gttgacgatc ccctggttgg aatcgatatc caaggcgtcc agtttagtat
     9181 tgccgtcaat gctgctcact ttcagcagca tggtgcgcac ggtgatgtcc gtgtcgcccg
     9241 tcacgcgcag ctgctcgccc ttaaactctt caatgttcag gttaagcggc aaatgcacgt
     9301 cggtcatttc cggcaatacc gggcgagaaa agagatcttt cagcgtttcg ccgagcggct
     9361 tttcctccgg ctgcggattt tcaattttcg gttcgaccac ttcttcctgc gccacttcca
     9421 ccactttcgg cagagcaatc agcaggcctt tcagcgacgt cggtttcagg gtcagggttt
     9481 tctcctgcca gttcaggccg gaggtgaagt ccatcaccga caccgtggtg tcatcaatct
     9541 tgatgttgac gttgtccagc gccacccgtg tcagggtgat gggatacggc gtggagagat
     9601 ccagcggacc gctatcttcc tcttcttcaa cctgttcaga aggaggcatt tttttactgt
     9661 cgatgttgac ctgaatgtct ttcagcgcca ggtcattaat acaaacgcta ctgttccaca
     9721 ggcactcaag accgaccgcc agatgcagat tacccgcttt taccgccacg cctagctgct
     9781 cataacgaac gtcagacaag gtgagatcgc gccagccgcc ggtgaccttg ccaatatcca
     9841 gtcctggcac ccagcgatcc gccgctttaa atacaagatg caggccgctg gtggtgccca
     9901 ccagaaacgc caccgatccc agcaacagta agataacgat aaccacgccg aggctgattt
     9961 ttttccataa actcataatt ctggccccag accgatgtaa aactgtaacc cgtgttcgtc
    10021 tttatccgcg accggtacgg caaaatcgag tttgattggc ccgaccggcg attcccagcg
    10081 tacgccgacc ccggtaccgg ttttaaagtc gctgcggcga atatcgctta ccgcttcgcc
    10141 actatcgaca aacaccgcgc cccaccattt tccggtcacg ttgtactggt attccagcga
    10201 tccggttatc aacttcgagg cccctttcag gtcaccgttg gcgtatttcg gggcgataga
    10261 tttgtatttg tagccgcgaa tactgcgatc gcccccgacg aagaaacgca gatccggcgg
    10321 tactttgtcg aaatcaccgg tttcaatcca gcccagcgta ccgcgtgtca caaagcgatg
    10381 gcgatcgtac agtgtgcgga tccagacgtt ctgcgcctgg aaaacggaga aatcgacatc
    10441 tgaaccccag gccgtgttgg agtagtcgat agagtagcgt tgcgagtctc cccaggttgg
    10501 catcaggcca ccacgagaac gcgtgcggct aatcatcacc ccaggataaa acagcatcgt
    10561 ggtgttggta atttcaccct gggtaaagtg gtcgaggctc cagcgcaggt taatggcacg
    10621 ctgccagccg ctggagagat cccagtagcg agaagccacc agcgtagtgg agtcagattc
    10681 ggtatcgttc aggtcagtgc gcttaaaacc gccctgcacc aaataatatt gttccagtgg
    10741 attcttcagc agcggcattt tatagctgaa gtcgagggtc tgttccggcg cggaaatact
    10801 ggtactggtg gtcaaactgt gaccgtatga gttcatccac ggctttttcc acgtcgcttt
    10861 cacgcgcggt cccacgtccg tagagtaacc gcccccggtt tcgatggtgt tttctgttcg
    10921 cggcgaaacc acgcccgtca atggtaatac tttcgtttcg cgcgctttat caaattgtgg
    10981 agc
//
LOCUS       DADMGC010000130        10876 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.130, whole genome shotgun sequence.
ACCESSION   DADMGC010000130 DADMGC010000000
VERSION     DADMGC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.130"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            126..1823
                     /locus_tag="JNZ73_003372"
     CDS             126..1823
                     /locus_tag="JNZ73_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAY8733537.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1957..2967
                     /gene="adhP"
                     /locus_tag="JNZ73_003373"
     CDS             1957..2967
                     /gene="adhP"
                     /locus_tag="JNZ73_003373"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HAY8733538.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVTDYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3113..3397
                     /locus_tag="JNZ73_003374"
     CDS             3113..3397
                     /locus_tag="JNZ73_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY8733539.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3625..4278)
                     /gene="fdnI"
                     /locus_tag="JNZ73_003375"
     CDS             complement(3625..4278)
                     /gene="fdnI"
                     /locus_tag="JNZ73_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HAY8733540.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4271..5155)
                     /gene="fdxH"
                     /locus_tag="JNZ73_003376"
     CDS             complement(4271..5155)
                     /gene="fdxH"
                     /locus_tag="JNZ73_003376"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY8733541.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5168..8215)
                     /gene="fdnG"
                     /locus_tag="JNZ73_003377"
     CDS             complement(5168..8215)
                     /gene="fdnG"
                     /locus_tag="JNZ73_003377"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7628..7630),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY8733542.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8447..9328
                     /gene="yddG"
                     /locus_tag="JNZ73_003378"
     CDS             8447..9328
                     /gene="yddG"
                     /locus_tag="JNZ73_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HAY8733543.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9587..10687
                     /gene="ompC"
                     /locus_tag="JNZ73_003379"
     CDS             9587..10687
                     /gene="ompC"
                     /locus_tag="JNZ73_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY8733544.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT         2673 a         2787 c         2689 g         2727 t
ORIGIN      
        1 gattaaacac cattgcctgc gcaatggtgt ttttatctgc tttatacttg gggacgacgc
       61 cctggcggta aagcaaagac gataaaagcg tgccagggat ggatattcaa aaaagagtga
      121 gtgacatgga accaaaaaca aaaaaacagc gttcgcttta tatcccttac gctggccctg
      181 tactgctgga atttccgttg ttgaataaag gcagtgcctt cagcatggaa gaacgccgta
      241 acttcaacct gctggggtta ctgccggaag tggtcgaaac catcgaagaa caagcggaac
      301 gagcatggat ccagtatcag ggattcaaaa ccgaaatcga caaacacatc tacctgcgta
      361 acatccagga caccaacgaa accctcttct accgtctggt aaacaatcat cttgatgaga
      421 tgatgcctgt tatttatacc ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct
      481 accgccgttc acgcggcgtg tttatctctt accagaaccg ccacaatatg gacgatattc
      541 tgcaaaacgt gccgaaccat aatattaaag tgattgtggt gactgacggt gaacgtattc
      601 tggggcttgg tgaccagggc atcggcggga tgggcattcc gatcggtaaa ctgtcgctct
      661 ataccgcctg tggcggcatc agcccggcgt atacccttcc ggtggtgctg gatgtcggaa
      721 cgaacaacca acagctgctt aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca
      781 ctgacgatga atactatgaa ttcgttgatg aatttatcca ggctgtgaaa caacgctggc
      841 cggacgtgct gttgcagttt gaagactttg ctcaaaaaaa tgcgatgccg ttacttaacc
      901 gctatcgcaa tgaaatttgt tcttttaacg atgacattca gggcactgcg gcggtaacag
      961 tcggcacact gatcgcagcc agccgcgcgg caggtggtca gttaagcgag aaaaaaatcg
     1021 tcttccttgg cgcaggttca gcgggatgcg gcattgccga aatgatcatc gcccagaccc
     1081 agcgcgaagg attaagcgag gaagcagcgc ggcagaaagt ctttatggtc gatcgctttg
     1141 gcctgctgac ggacaagatg ccgaacctgc tgcctttcca gaccaaactg gtgcagaagc
     1201 gcgaaaacct cagtgactgg gataccgaca gcgatgtgtt gtcactgctg gatgtggtgc
     1261 gcaatgtaaa accagatatt ctgattggcg tctcaggaca gaccgggctg tttacggaag
     1321 agatcatccg tgagatgcat aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc
     1381 cgacgtctcg cgtggaagcc acaccgcagg acattatcgc ctggaccgaa ggtaacgcgc
     1441 tggtcgccac tggcagcccg tttaatcctg tggtatggaa agataaaatc taccctatcg
     1501 cccagtgtaa caacgccttt attttcccgg gcatcggcct gggtgttatt gcttccggcg
     1561 cgtcacgtat caccgatgag atgctgatgt cggcaagtga aacacttgct cagtattcgc
     1621 cgctggtcct gaacggcgaa ggtctggtac taccggaact gaaagatatt cagaaagtct
     1681 cccgcgcaat tgcgtttgcg gttggcaaaa tggcgcagca gcaaggcgtg gcggtcaaaa
     1741 cgtctgctga agctttgcaa caagccattg acgataattt ctggcaagcc gaataccgcg
     1801 actaccgccg tacctccatc taagcctgca cccggtagtg aaggctaccg ggctatttcc
     1861 ctctcccttt ttcagatctc atccatactg ggtagtggcg aataaatctc atttgcctca
     1921 cctgctatgc agaacatcat ccgaaaagga ggaactatga agactgcagt tgttacgaag
     1981 gatcatcatg ttgacgttac ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg
     2041 ctgaaaatgg agtgttgtgg tgtatgtcat accgatcttc atgttaagaa tggcgatttt
     2101 ggtgacaaaa ccggcgtaat tctgggccat gaaggcatcg gtgtggtggc agaagttggc
     2161 ccaggtgtca cctcattaaa accaggcgat cgtgccagcg tggcgtggtt ttacgaagga
     2221 tgcggtcatt gcgaatactg taacagtggt aacgaaacgc tctgccgttc agttaaaaat
     2281 gccggataca gcgttgatgg cgggatggcg gaagagtgca tcgtggtcac cgattacgcg
     2341 gtaaaagtgc cagatggtct ggactcggcg gcgcccagca gcattacctg tgcgggggtc
     2401 accacctaca aagccgttaa actgtcaaaa attcgtcccg gtcagtggat tgctatctac
     2461 ggtcttggcg gtctgggtaa cctcgccctg caatacgcaa agaatgtctt taacgccaaa
     2521 gtgatcgcca ttgatgtcaa tgatgagcag ttaaaactgg caaccgaaat gggtgcagat
     2581 ttagcgatta actcacgcac cgaagacgcc gccaaaattg tgcaggagaa aaccggtggc
     2641 gctcacgctg cggtggtaac agcagtagct aaagctgcgt ttaactcggc agttgatgct
     2701 gtccgtgcag gcggtcgtgt tgtggctgtc ggtctgccgc cggagtctat gagcctggat
     2761 atcccacgtc ttgtgctgga tggcattgaa gtggtcggtt cgctggtcgg cacgcgccag
     2821 gatctaaccg aagccttcca gtttgccgcc gaaggcaaag tggtgccgaa agtcgccctg
     2881 cgtccgttag cggacatcaa caccatcttt accgagatgg aagaaggcaa aatccgtggc
     2941 cgtatggtga ttgatttccg tcactaagag gtctttgctg cgactcccat gttcgggtcg
     3001 cagcatcgca cactctccaa catgaaatgg ctaaatggat tattgttgcg taggaaggaa
     3061 caataatgat agtgttccca gagaagtaac gctactgaga gtaaagacac taatgaaaat
     3121 ggccaatcat ccccgcccgg gggacattat tcaggaatca ctggacgaac ttaatgtcag
     3181 cctgcgcgag tttgccagag caatggaaat tgcgccctca acggcaagcc gattgctgac
     3241 cggaaaagca gctttgacgc cagaaatggc aattaaactt tccgtggtga tcggcagttc
     3301 gccgcaaatg tggctgaatc tgcaaaacgc ctggagtctg gcagaggcag aaaaaacggt
     3361 ggttgtgtcg cgattacgcc gtctggttac gcaataaaaa atggcacctt ataaagcgcc
     3421 atttgaaact gatgacaaac gcaacattgc ctgatgcgct acgcttatca ggcctacacg
     3481 ttcacttcaa tctattgtat ttgaagggtt ttcatgccgg ataaggcatt cacgacgcat
     3541 ccgggatgaa caaagcccac gttgtctaca atatgaaaat ggcgcctttt acagcgccat
     3601 tttcagctta aagttcaaaa gagattatat cccttcttca ctctcttttt tcgcttctgc
     3661 cttctcgatt tcacgatacc agcgcggatg gtgtttcttc gcccagcgac ggctcacctt
     3721 cccttcgatc atccctttaa tcgacccttt cacccagaat gccatataca tatggatcag
     3781 gatggcgtgg atcaggatga tacccgcagc cgcgtggatc agcaggctgt agcgaacaac
     3841 ctgcatcggg aagtactgcg caaagtacgg acgccagata atcaccccgg tcaccagcag
     3901 cacgaaaatc atgctcatga tcgaccagaa catcatcttt tgcccggcgt tgtacttacc
     3961 aacatccgcc actttgtgct cattgccttt taatacttcg acaatgttca acagccacgg
     4021 aatatctttc ttatccggga tgttgtgatg cacaaaacgc acaaacataa acatcagtgc
     4081 gacgaaaatc gcaatgccga agaacgggtg caaaatgcgt cccatctgcg gcgtaccgaa
     4141 ggtttgcgtc agccattgca gcgtcgggaa gaagaacgaa atcccggaca gcgccaccag
     4201 gaagaagcaa atcaccacgg tccagtgaca ggcgcgatca ataaatttgg tgcgcacaat
     4261 cattttcgac ttactcatga tgatcctcct cgtcatcgtc cacttcctta ttcgggccaa
     4321 taccgatgta gtggaaaatc aacccggcaa aagtggcaat aaagccagcc gctgccagcg
     4381 gtttcaacgc gcctttccac aggcttaccg aggtgtcgat cttcggatct ttcggcagac
     4441 cgtgatacag ctccggctga tcggcgtgat gcagcacgta cataacgtgc gtaccaccga
     4501 ccccttccgg gttgtagacg ccagcatgtt cgtaaccacg cgctttcagt ttcgccacgc
     4561 gctgttccgc cagctccagc atctccttct tggtgccgaa gtggatagcc ccggtcggac
     4621 aggttttcac acaagccggt tcctggccga cgctgacgcg atcgacgcag agcgtgcatt
     4681 tatatacccg gttatcctct ttgttgaggc gcggaatatt aaacggacac ccggcaatgc
     4741 agtaaccaca gccgatgcag ttttccgact ggaaatcgac aatcccgtta gcgtactgaa
     4801 tgattgcacc agcagacggg cacgccttca ggcagcccgg atcttcacag tgcatacagc
     4861 cgtctttacg gatcagccac tccagcttgc cattctgttc ggtttcgcta aagcgcatca
     4921 ccgtccagga cttggcgctc agatcggcgg ggttatcgta aacaccgacg cagtgcccca
     4981 cttcatcacg gatgtcgttc cactccgaac acgccacctg acaggcttta cagccgatac
     5041 aggtggaaac gtcgataagt ttcgcgactt ctgctttgta atcacgcacc tgagaaggcg
     5101 gcgtgatgga gttagttgcg gaccttttga taatatcctg cgtttccata gccatctgtt
     5161 cgccccctta cgccttctcg atgttgacta agaacgcttt atattccggc gtttgcgagt
     5221 ttgcatcacc gacattcggc gtcagagtgt tagcgatata acctttacgc gcgacaccct
     5281 caaagcccca gtggattgga atacccaccg tttcaacctg ctgaccgttt acattcagcg
     5341 gtttcagacg gcgcgttacc actgccaccg cgcggataaa gcctcgcttg ctggagacag
     5401 tgacacgatc gccattatta atgcctttcg ccgccgcctg cgtttcgctg atttccacaa
     5461 actgttccgg ctgagcaatt gcgttgagca acgcgtgctt ggtccaggtg tggaagtgct
     5521 cggtcagacg ataggtcgta cccacatacg ggaactgctc ttttttaccc atccgcagcg
     5581 cgtcttgttc atacagacga acaaccgggt tagacaccac gttcggatgc agcgggttag
     5641 tgcccagcgg cgtttcaatc ggctcgtagt gttccgggaa cggaccttcc gccattttgt
     5701 tgatggcaaa caggcgtccc atcccttccg gctgcataat aaacggcccg gttggcgtac
     5761 ccggtgcggc attgccgaag tccggaatat cgttacccgt ccacttgctg ccgttccact
     5821 ggatcagcat ccgtttcgga tcccacggtt taccgttgat atccgccgaa gcacggttgt
     5881 agagtacgcg acggttgagc ggccacgccc aggcccatcc cagcgtattc cccagaccgg
     5941 acgggtcgga gttatcgcgg tttgccatct ggttaccctg ctctgtccag ctaccggtgt
     6001 agatccagca agaagatgcg gttgtaccgt catcacgcag atgcgcaaag ctactcagca
     6061 actgaccttt cttcgcaatc agcacgccat tagcatcata gagatcttcc aacgcatagc
     6121 cgttgttctc tttagctact tcgtcagatt gcggttcgtg cggctgcttg tagttccagc
     6181 tcatcttcat cagcggttct acgcctttac caccttcggc ctggtacaac tcgcgcagat
     6241 gatggtagat acccgccaga atttcgccgt cgttacgcgc ttcgcccggc gcgtcctgac
     6301 ctttccagtg ccactgcaac cagcgaccgg agttagcgat agaaccatcc tcttcagcaa
     6361 agcaggtcga aggcagacgg aatacttcag tctgaataga cgccggatcg acatcgttcg
     6421 actcgccgtg gttctgccag aaggtagagg tttcagtcac cagcggatcg ataaccacca
     6481 tgtacttcag tttgctcagg cagctcacca ctttgttttt atccgggaag gacgcaaccg
     6541 ggttaaagcc ctggcagaaa taaccggtga ctttgccttc atccatcatg ttgaaatact
     6601 tgatgacgtc gtaggtctgg tcccacttcg gcagccagtc atagccccag ttgttctctt
     6661 tctgcgcggc atcgccatag aaagatttca tcaggctaac gaagaacttc ggatagttgc
     6721 tccagtagtt cacctgatca gccagcgtcg ctttcggcgt gttcgcttcc agatacgact
     6781 gcaaatcaac ctgtttttct gacggcagcg tcagataacc tggcaggctg gtagagagca
     6841 gacctaagtc agtcagcccc tgaatgttgg agtgaccacg caatgcgttc acgccgccac
     6901 cggccatacc catgttaccg agcagcaact ggatcatcgc catagtacgg atgttctgcg
     6961 cacccacggt gtgctgcgtc cagcccagcg cgtacaggaa ggtggttgtg cgatccggtg
     7021 cgctggtgga ggccagcact tcacacactt tcaggaagtc ggcttttggc gtaccacaga
     7081 tgttttcaac aacgtccggc gtgtagcggg aaacgtgcgc tttcagcagg ttccacacac
     7141 agcgcggatg agtcagtgtt tcatcgcgtt tcgcatagcc gttttcatcg aactgatagt
     7201 tccaggacga tttgtcgtac tggcgttttt cagcgtcata accgctgaac aggccatctt
     7261 cgaaagcaaa atcatcacgc accagtaggc tggcgttggt gtaatgttta acgtattcgg
     7321 cgttgatttt gttgttttcg atcaggtagc gcaaaacgcc agacaggaac gtaatgtccg
     7381 taccggaacg aataggtgcg taaatatccg ccacagaagc ggtacgcgta aaacggggat
     7441 cgacaacgat caaggttgca tcgttgttgt ttttcgcttc catcgcccag cggaaaccga
     7501 cgggatgcgc ttcagcagcg ttaccgccca ttaccattac gacgttagcg tttttgatat
     7561 ccacccagtg gttggtcata gcacctcgac caaatgttgg agcaagactt gctaccgttg
     7621 gtccgtgtca gacgcgcgcc tggttgtcta ccgccagcat cccgagggag cgggcaaatt
     7681 tctgggtcag catcccggtt tcgttgctgg caccggaggc acacagcata ccggtagaaa
     7741 gccaacggtt taccgttacg ccctgctcgt tcttttcaat aaagttagcg tcacggtcag
     7801 ctttcatcag cttcgcaata cgggagaatg cttcttccca gctaatgcgc tgccatttgt
     7861 cagaacctgg cgcacgatat tccgggtagc gcagacggtt ttcactgttg acgtaatcca
     7921 gcaaaccggc ccctttcggg cacagcgcac cacggcttac cggatgatcc gggtcacctt
     7981 caatgtgata aatcgcttct ctggcgtttt ttgcgccatc acccaggcta tacatcaata
     8041 gcccgcaacc tacggaacag tatgtgcagg tgttacggat ctctttagcg cgtaataatt
     8101 tgtagtttcg cgcctgagcc agtgcttgct tcggggcaaa gcccaatgcc gctaccgttg
     8161 ttccagccat acctcccgcg cagattttaa aaaattgtct gcgactgacg tccattgctt
     8221 tcctcttttt tcaggggtat tactgcgcga ggaaactaac agtaaaattc tgaatctttt
     8281 tgcgcgaaat caaaaagaac acgtgtttgc cacaaaataa tccgcctgcg caaattgacc
     8341 tacctcaata gcggtagaaa aacgcaccac tgcctgacag gccagttaaa aaaatgctat
     8401 aaaattcagc ttaattttta acggcaagag agacaaaaca gcgagcatga cacgacaaaa
     8461 agcaacgctc atagggctaa tagcgatcgt cctgtggagc acgatggtag gattgattcg
     8521 cggtgtcagc gaggggctcg gcccggtcgg cggcgcagct gctatctatt cattaagcgg
     8581 gctgctgtta atcttcacgg ttggatttcc gcgtattcgg cagattccga aaggctattt
     8641 actcgccggg agtctgttat tcgtcagcta tgaaatctgt ctggcgcttt ccttagggta
     8701 tgcggcgacc cgtcatcagg cgattgaagt gggtatggtg aactatctgt ggcccagcct
     8761 gacaattctc tttgccattc tgtttaatgg tcagaaaacc aactggctga ttgtaccagg
     8821 attattatta gccctggtcg gcgtctgctg ggtgttaggc ggtgagaatg ggttacatta
     8881 tgatgaaatc atcaataata tcaccaccag cccattgagt tatttcctgg cgtttattgg
     8941 tgcgtttatc tgggcagcct attgcacagt aacgaataaa tacgcacgcg gatttaacgg
     9001 aattaccgtt tttgtcctgc taacgggagc atgtctgtgg atttactatt ttctcacgcc
     9061 acaaccagaa atggtattta gcacgcccgt catgattaaa ctcatctctg cggcatttac
     9121 cttaggattt gcttatgctg catggaatgt cggtatattg catggcaatg tcaccataat
     9181 ggcggtaggc tcgtatttta cgcctgtact ttcctcagcg cttgcagccg tgctgctcag
     9241 cgccccgctg tcgttctcgt tctggcaagg cgcgctaatg gtctgcggcg gttccctgct
     9301 ctgctggctg gcgacacgtc gtggttaaaa taattgccgg gtctacggcc cggcagttaa
     9361 tcagataaat tccgacaata ttaaaaaata aatttcaaat accagtaatt caccttatat
     9421 ctcatttcga cttcgatcac atatcgataa ataatattat ttcatctatt tttctcattg
     9481 tttttaatca ccattctatt ttaaaaacag tatgtcattt ataaatgacg ccgtttaaaa
     9541 ttcgtttaga aaatacgttt aacgataatt aaaaggattc tgttaaatga aattaaaaat
     9601 agttgcggtg gttgtaactg gtttgttagc tgcgaacgta gcacacgctg ccgaagtcta
     9661 taacaaggat ggtaataaac tcgaccttta tggcaaggtt accgctctac gttattttac
     9721 tgatgataag cgtgacgatg gtgataaaac ttatgcccgt ctcggcttta aaggagaaac
     9781 gcaaatcaat gatcaaatga ttggttttgg tcactgggaa tatgatttta aaggctataa
     9841 cgatgaagcc aacggctcgc gcggcaacaa gacccgtctg gcctatgctg gtttaaaaat
     9901 tagtgaattt ggctctctgg actatggtcg taactacggt gtcggctacg acattggttc
     9961 atggaccgat atgttgccag aatttggtgg cgatacctgg agtcagaaag atgtcttcat
    10021 gacataccgt accaccggtc tagcaaccta tcgcaactac gatttctttg gcttaattga
    10081 agggctgaac tttgccgcgc aatatcaagg caaaaatgaa cgtactgaca acagtcatct
    10141 ttatggtgct gactacacgc gtgccaacgg tgacggtttc ggtatctcct caacttatgt
    10201 ttatgatggc tttggtatcg gtgcggtata taccaaatcc gatcggacaa atgcgcagga
    10261 aagagccgct gctaatcctc tcaatgcctc cggtaagaat gcagaactat gggccacagg
    10321 tataaagtat gatgccaaca acatctactt tgcagctaat tacgctgaaa cattaaacat
    10381 gaccacctat ggggatggtt atatctctaa caaagcacaa agttttgaag tggtggcgca
    10441 atatcaattc gacttcggct tgcgcccatc actcgcttac ctgaaatcga aaggcagaga
    10501 tctgggccgc tacgccgacc aggacatgat tgagtatatc gacgttggtg cgacgtattt
    10561 cttcaacaaa aatatgtcga cctatgttga ttataaaatc aacctgattg atgaaagcga
    10621 ctttacccgt gccgtagata ttcgcaccga taacatcgtc gctacgggca ttacctatca
    10681 gttctaacgt cctctcctga ccagcccttc tctgggctgg tttttgattt aagtgctgct
    10741 gtatgactcc ttaagtaaaa atgagcaatt tttgatattt ctggtcattc acctcaatta
    10801 atattattcc atatcctccg gggacatctt actataatat ttattgatga gtgattatga
    10861 ccaatattaa tacagc
//
LOCUS       DADMGC010000131        10809 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.131, whole genome shotgun sequence.
ACCESSION   DADMGC010000131 DADMGC010000000
VERSION     DADMGC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.131"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            87..1172
                     /locus_tag="JNZ73_003380"
     CDS             87..1172
                     /locus_tag="JNZ73_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY8733545.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            1243..1851
                     /locus_tag="JNZ73_003381"
     CDS             1243..1851
                     /locus_tag="JNZ73_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAY8733546.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1949..3340
                     /gene="selA"
                     /locus_tag="JNZ73_003382"
     CDS             1949..3340
                     /gene="selA"
                     /locus_tag="JNZ73_003382"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAY8733547.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVTHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3337..5181
                     /gene="selB"
                     /locus_tag="JNZ73_003383"
     CDS             3337..5181
                     /gene="selB"
                     /locus_tag="JNZ73_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAY8733548.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPER"
     gene            5371..6522
                     /gene="yiaY"
                     /locus_tag="JNZ73_003384"
     CDS             5371..6522
                     /gene="yiaY"
                     /locus_tag="JNZ73_003384"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HAY8733549.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            6687..8225
                     /locus_tag="JNZ73_003385"
                     /pseudo
     CDS             6687..8225
                     /locus_tag="JNZ73_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(8444..8599)
                     /locus_tag="JNZ73_003386"
     CDS             complement(8444..8599)
                     /locus_tag="JNZ73_003386"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733550.1"
                     /translation="MAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRP
                     LTIETRD"
     gene            8770..9093
                     /locus_tag="JNZ73_003387"
     CDS             8770..9093
                     /locus_tag="JNZ73_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY8733551.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLISKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            9099..10235
                     /locus_tag="JNZ73_003388"
     CDS             9099..10235
                     /locus_tag="JNZ73_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY8733552.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(10232..>10809)
                     /locus_tag="JNZ73_003389"
     CDS             complement(10232..>10809)
                     /locus_tag="JNZ73_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8733553.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         2577 a         2656 c         2975 g         2601 t
ORIGIN      
        1 gcactttctg accgaatttc ccggctggag catcaactcg ccgccgagaa aaagactgac
       61 tattccacgt tcccggagat ctaattatgg atctattgat tgttttaact tacgtggcgc
      121 tggcgacggc ggcgctggga ggcgtgtttc tggtgagtgg tttgattttg ttgatgaact
      181 acaaccaccc ttacactttt accgcgcaaa aggcagtgat agcgatccct atcacgccac
      241 aggtgacggg aattgttact gaagtcactg acaagaataa tcagcttatt caaaagggcg
      301 aggtgctttt taagctcgac ccggttcgtt accaggcgcg agttgacaga cttcaggctg
      361 acctgatgac ggcgacgcat aatataaaga cgctgcgtgc gcagctcact gaagcgcagg
      421 ccaacaccac ccaggtttca gcggagcgcg accgtctgtt taaaaattat caacgttacc
      481 tgaaaggcag ccaggcggcg gtgaatccgt tctcggaacg tgacatcgac gatgcgcggc
      541 aaaatttcct cgcgcaggat gcgttagtga aaggctcggt ggcggagcag gcgcagatcc
      601 agagtcagct cgacagtatg gtcaacggcg agcaatcgca gattgtgagc ttaagagcgc
      661 aacttactga agcaaaatat aatcttgagc agactgtcat tcgcgcacca agcaatggct
      721 acgtcactca ggtactgatc cgcccaggta catacgcagc tgccttgccg ctgcgtccgg
      781 tgatggtctt catccccgag caaaaacggc aaattgtcgc ccaatttcgg caaaactcgc
      841 tgttacgtct gaaacctggc gatgatgcgg aagtggtgtt taacgcgcta cctgggcagg
      901 tgttccacgg caaattgact agtattttac ctgtcgtgcc aggcggttct tatcaggcgc
      961 agggggtatt gcaatcatta acggtcgttc ccggcacgga cggtgtgctg ggaaccattg
     1021 aactggaccc taacgatgat atcgatgcct tacccgacgg catctacgcc caggtggcgg
     1081 tttactccga ccatttcagc catgtttcgg tgatgcggaa agtgctgcta agaatgacca
     1141 gttggatgca ttatctttat ttggatcatt gagacttata tcacctgtgt tttgcgtgag
     1201 cctgagccat actgatattt ttcctcgcac aaaggactcg gcatgaaact cgtcggtagc
     1261 tacaccagcc cgtttgtacg caaactttct attctgttgt tagaaaaggg cataactttc
     1321 gaatttatta atgaactgcc ctataacgcg gataacggcg tggcgcaatt taacccgtta
     1381 gggaaagtgc cggtgctggt gaccgaagag ggcgaatgct ggtttgattc gccgatcatc
     1441 gctgaatata ttgagttaat gaatgtcgct ccggcgatgt tgccgcgcga tccgctggag
     1501 tcgttgcggg tgcgcaaaat tgaggcgctg gcggatggca ttatggatgc cgggctggta
     1561 tcggtgcgtg aacaggcgcg tccggcggcg cagcagtctg aagatgaatt gttacgccag
     1621 cgggagaaaa tcaaccgcag tctggatgtg ctggaaggat atctggtcga tggcacactc
     1681 aaaaccgata cggtcaatct ggcgactatc gccattgcct gtgctgtcgg atatctcaat
     1741 ttccgccgcg ttgcgccagg ttggtgtgtc gaccgcccac acttagtcaa actggtcgaa
     1801 aacctgttta gccgcgaaag ttttgctcgc accgaaccgc caaaggcttg atgcgcgata
     1861 tgtcctcctg acccatctca cgttacaatc cgtggttatg ttaaacgccc ttctccgtgt
     1921 gagagggcct tgatcagcca ggtttcctat gacaaccgaa acgcgttccc tctatagtca
     1981 acttccggct attgatcgct tattgcgcga tagctccttc ctttctttgc gtgatactta
     2041 tggtcacacc cgcgtggtgg aattgttgcg tcagatgctc gacgaagcgc gagaagtgat
     2101 tcgtggcagc cagacgctgc ctgcgtggtg tgaaaactgg gcgcaagaag tcgatgcccg
     2161 gttgacgaaa gaggcgcaga gcgcgctgcg tccggtgatc aacctgacgg gaaccgtgct
     2221 gcataccaac cttgggcgag ctttacaggc ggaagccgcg gtggaagccg ttgcgcaggc
     2281 tatgcgttcg ccagtgaccc tcgagtacga tctggacgac gccggacgcg gacatcgcga
     2341 tcgggcgctg gcgcagctgc tgtgccgtat tacgggggcg gaagatgcct gtatcgtcaa
     2401 taacaatgcg gcggcggtgt tattgatgtt ggcggccact gccagcggga aagaggtggt
     2461 ggtatctcgc ggcgaactgg tggagattgg cggcgcgttt cgtattcccg atgttatgcg
     2521 tcaggcaggc tgcaccctac acgaagtagg gaccaccaac cgcacgcacg cgaatgatta
     2581 tcgtcaggcg gtgaatgaaa ataccgcact gttgatgaaa gtacatacca gtaactacag
     2641 cattcagggg ttcaccaaag cgatagacga agctgaactg gtggcgctcg gcaaagagct
     2701 ggatgttcca gtggtgactg atttaggcag tggctcgctg gtcgatctta gccagtacgg
     2761 tttgccgaaa gagccaatgc cgcaggagtt gattgcggcg ggcgtcagtc tggtgagctt
     2821 ctccggcgac aagttgttag gcgggccgca ggcagggatt atcgttggta aaaaagagat
     2881 gatcgcccgc ctgcaaagcc acccgctgaa gcgtgcatta cgcgcggata aaatgaccct
     2941 cgcggcgctg gaagccacgt tgcgtcttta tttacaccct gaagctctga gtgaaaaatt
     3001 accgaccctg cgcctgctta cccgcagcgc agaggtcatt caaatccagg cacaacgttt
     3061 acaggctccc cttgtcacac attacggcgc ggagtttgcg gtacaggtta tgccatgtct
     3121 ttcgcagatt ggcagtggtt cgctgccggt tgatcgcctg ccgagcgcgg cattaacgtt
     3181 tacaccccat gatggacgcg gtagccacct tgagtcatta gccgcccgct ggcgtgaatt
     3241 gccagtgccg gtgattggtc gtatttatga cggacgattg tggctggatt tacgctgcct
     3301 tgaagatgag caacggtttt tggagatgtt gttgaaatga ttattgcgac tgccggacac
     3361 gttgaccacg gcaaaacaac cttattgcag gcgattactg gcgtaaatgc tgaccgtctg
     3421 ccggaagaaa aaaagcgcgg catgaccatc gatctcggct atgcctactg gccgcagccg
     3481 gatggtcgcg tgcctggttt tatcgacgtt cccggtcatg aaaagtttct ttccaacatg
     3541 ctggcgggcg ttggtggtat cgatcacgcg ctgttggtgg tggcgtgcga tgacggcgtg
     3601 atggcacaga cccgtgagca tctggcgatt ttgcagctga ccggtaaccc gatgctgaca
     3661 gtggcgctga ccaaagccga tcgtgtggac gaagcgcgtg ttgatgaggt tgaacgccag
     3721 gtaaaggagg ttctgcggga atacggtttt gctgaggcaa aactgtttat caccgcagca
     3781 accgaaggtc ggggaattga tgccctgcgc gagcatctgc ttcagttgcc ggaacgcgag
     3841 cacgccagcc aacatagttt ccgcctcgcg attgaccgcg catttaccgt aaaaggtgcc
     3901 gggctggtcg tcaccggtac ggcgttaagc ggggaagtga aggtaggcga ttcactctgg
     3961 ctgactggtg taaataaacc gatgcgtgta cgtgcgctgc atgcgcaaaa ccagccaaca
     4021 gaaaccgcca atgccgggca gcgtatcgcg cttgacatcg cgggtgatgc ggaaaaagag
     4081 cagattaacc gtggcgactg gctgcttgcc gatgtgccgc cagagccgtt cacacgggtg
     4141 attgtcgagc ttcaaaccca tgcaccgctg acccagtggc agccgctgca tattcaccac
     4201 gccgccagcc acgtcacggg acgcgtttca ctgctggaag ataaccttgc tgaactggtc
     4261 ttcgacaccc cgttatggct ggcagataac gaccgcctgg tattgcgcga tatctctgcc
     4321 cgcaacacgc tggccggagc gcgcgtcgtg atgcttaacc cgccgcgtcg cggtaaacgt
     4381 aaaccagagt atctgcaatg gctggcttcc ctcgctaggg cacagaatga tagcgaagcg
     4441 ttagccgttc acctgcaacg cggcgcggtt aatcttcctg atttcgcctg ggcgcgccag
     4501 ctcaacggcg aagggatgcg cgaactgctg caacagcctg gctatattca ggctggttat
     4561 agcttgttga atgcgccggt tgccgctcgc tggcaacgga aaattctcga cacattagcg
     4621 acttatcatg agcaacatcg cgatgaacct gggcctgggc gcgaacgtct gcgacgtatg
     4681 gcgttgccaa tggaagatga agcgctagta ctgttgctga ttgaaaagat gcgcgaaagc
     4741 ggcgacatcc tcagtcatca cggttggctg catctgccgg atcacaaagc gggcttcagc
     4801 gaagagcagc aggccatctg gcaaaaagca gagccgctgt ttggtgacga gccgtggtgg
     4861 gtgcgtgatc tggcaaaaga gacgggtacc gacgagcagg caatgcgcct gacgctacgc
     4921 caggcggcgc agcaaggaat aattaccgcg atcgtcaaag atcgttatta ccgtaacgat
     4981 cggattgtcg aatttgccaa tatgatccgc gatctcgatc aggagtgtgg ttcaacctgc
     5041 gcggcggatt tccgcgatcg cttaggcgta ggccgaaagc tggcaattca gattctggaa
     5101 tattttgacc gcattggctt tacgcgtcgt cgtggaaatg atcatttatt gcgcgacgca
     5161 ttattatttc cggaaagata aggaaatgat taattaattt ttaaaataaa ttaatacaaa
     5221 tttcttatga ttttaaataa agcacattgt ttaatgaata caatgtgctt tttattagat
     5281 taattttgat aattaataat aatgatcatg ttttcgaatg aaaatcctca gtaagctgcc
     5341 cacccttttt tacactttca ggagtgtgtt atggcatctt caactttctt tattccttct
     5401 gtgaatgtca tcggcgctga ttcattgact gatgcaatga atatgatggc agattatgga
     5461 tttacccgta ccttaattgt cactgacaat atgttaacga aattaggtat ggcgggtgat
     5521 gtgcaaaaag cactggaaga acgcaatatt tttagcgtta tttatgatgg cacccaacct
     5581 aaccccacca cggaaaacgt cgccgcaggt ttgaaattac ttaaagaaaa taattgcgat
     5641 agcgtgatct ccttaggcgg tggttctcca cacgactgcg caaaaggtat tgcgctggtg
     5701 gcagccaatg gcggcgatat tcgcgattac gaaggcgttg accgctctgc aaaaccgcag
     5761 ctgccgatga tcgccatcaa taccacggcg ggtacggcct ctgaaatgac ccgtttctgc
     5821 atcatcactg acgaagcgcg tcataacaaa atggcgattg ttgataagca cgtgactccg
     5881 ctgctttctg tcaatgactc ctctctgatg attggtatgc cgaagtcact gaccgccgca
     5941 acgggtatgg atgccttaac gcacgctatc gaagcgtatg tttctattgc cgccacgccg
     6001 atcactgacg cttgtgcact gaaagccgtg accatgattg ccgaaaacct gccgttagcc
     6061 gttgaagatg gcagtaatgc gaaagcgcgt gaagcaatgg cttatgccca gttccttgcc
     6121 ggtatggcgt tcaataatgc ttctctgggt tatgttcatg cgatggcgca ccagctgggc
     6181 ggtttctaca acctgccaca cggtgtatgt aacgccgttc tgctgccgca tgttcaggta
     6241 ttcaacagca aagtcgccgc tgcacgtctg cgtgactgtg ccgctgcaat gggcgtgaac
     6301 gtgacaggta aaaacgacgc ggaaggtgct gaagcctgca ttaacgccat ccgtgaactg
     6361 gcgaagaaag tggatatccc ggcaggccta cgcgacctga acgtgaaaga agaagatttc
     6421 gcggttctgg cgactaatgc cctgaaagat gcctgtggct ttactaaccc gatccaggca
     6481 actcacgaag aaattgtggt gatttatcgc gccgcaatgt aatcatcatt ccaacaacgg
     6541 ctggcaaatt gtcagccgct ttttcaacta tctctgtaat ccttgcccgt aaattcgtga
     6601 tagctgtcgt aaagctgtta ccgactggcg aagatttcgc cagtcacgtc tacccttgtt
     6661 atacctcaca ccgcaaggag acgatcatga ccaataatcc cccttcagca cagattaagc
     6721 ccggcgagta tggtttcccc atcaagttaa aagcccgcta cgacaacttt attggcggtg
     6781 aatgggtagc ccctgccgat ggtgagtatt accagaacct gacgccggtg accgggcagc
     6841 tgctgtgcga agtggcgtct tcgggcaaac gagacatcga tctggcgctg gatgctgcgc
     6901 acaaagtgaa agataaatgg gcgcacacct cggtgcagga tcgcgcggcg attctgttta
     6961 agattgccga tcgaatggaa caaaacctcg agctgttagc gacagctgaa acctgggata
     7021 acggcaaacc cattcgcgaa accagtgctg cggatgtacc gctggcgatt gaccatttcc
     7081 gctatttcgc ctcgtgtatt cgggcgcagg aaggtgggat cagtgaagtt gatagcgaaa
     7141 ccgtggccta tcatttccat gaaccgttag gcgtggtggg gcagattatc ccgtggaact
     7201 tcccgctgct gatggcgagc tggaaaatgg ctcccgcgct ggcggcgggc aactgtgtgg
     7261 tgctgaaacc cgcacgtctt accccgcttt ctgtactgct gctaatggaa attgtcggtg
     7321 atttactgcc gccgggcgtg gtgaacgtgg tcaatggcgc aggtggggaa attggcgaat
     7381 atctggcgac ctcgaaacgc atcgccaaag tggcgtttac cggctcaacg gaagtgggcc
     7441 aacaaattat gcaatacgca acgcaaaaca ttattccggt gacgctggag ttgggcggta
     7501 agtcgccaaa tatcttcttt gctgatgtga tggatgaaga agatgccttt ttcgataaag
     7561 cgctggaagg ctttgcactg tttgccttta accagggcga agtttgcacc tgtccgagtc
     7621 gtgccttagt gcaggagtca atctacgaac gctttatgga acgcgccatc cgccgtgtcg
     7681 aaagcattcg cagcggtaac ccgctcgaca gcgtgacgca aatgggcgcg caggtttctc
     7741 acgggcaact ggaaaccatc ctcaactaca ttgatatcgg taaaaaagag ggcgctgacg
     7801 tgctcacagg cgggcggcgc aagctgctgg aaggtgaact gaaagacggc tactacctcg
     7861 aaccgacgat tctgtttggt cagaacaata tgcgggtgtt ctaggaggag atttttggcc
     7921 cggtgctggc ggtgaccacc ttcaaaacga tggaagaagc gctggagctg gcgaacgata
     7981 cgcaatatgg cttgggcgcg ggcgtctgga gccgcaacgg taatctggcc tataagatgg
     8041 ggcgcggcat acaggctggg cgcgtgtgga ccaactgtta tcacgcttac ccggcacatg
     8101 ctgcgtttgg tggctacaaa caatcaggta tcggtcgcga aacccacaag atgatgctgg
     8161 agcattacca gcaaaccaag tgcctgctgg tgagctactc ggataaaccg ttggggccgt
     8221 tctgatataa gaagctggtc gcattgggta ttcattgcct gatgcgacgc ttacgcgtct
     8281 tatcatacct acgggaacct gaccgtaggc cggataaggc gtacacgccg catccggcat
     8341 aaacaaagcg cacgttgtta caatttacgt tttaaaccta cgtgcgcaat aacccagact
     8401 acgccaacag aagtgcgttt ctggggcgtg ttttgtcagg agtttaatct cgcgtttcga
     8461 tggtgagcgg gcgaaccagg ccgtaaatca ccattggtga tccgaggcca atcagcaata
     8521 aacggtagat aattgaaggt ttaatacgct taatctgtac tgtggatgtt ttactggagt
     8581 tattcattag tttggccaat agttccacta tttcaatatg ataaacctcg tcctgttgaa
     8641 aaaccagaca gcacatttca gaaagtatat aaaaaacaat atgtcgttat ataaataagg
     8701 aaattgcact gcatcatggc atttcataat atgcgttaat tgttcttttt tatattcttt
     8761 gagggaaata tgttcctgga ctattttgca ctgggagtgc ttatttttgt atttctggtg
     8821 atattctatg ggattattat tttacatgat attccctacc tgatttctaa aaaacgtaat
     8881 catcctcatg ccgacgccat tcatgttgct ggttgggtga gtctttttac gctgcatgtt
     8941 atctggccat ttttgtggat ttgggccacg ctttaccgcc cggagcgggg atggggaatg
     9001 caaatccatg actcctccgt tgtgcaacta caacagcgta ttgccgggct ggagaaacag
     9061 ctcgccgaca tcaaatcctc ttctgccgag taatacttat ggatttactg attattttga
     9121 cctatgtggc ttttgcatgg gcaatgttta agatcttcaa aattcctgta aataaatgga
     9181 ccatttccac agcggccctg ggtggcatat ttattgtcag tggtttaatt ctgttaatga
     9241 actataacca tccgtatacc ttcaaggcgc aaaaagcggt tatttctatt cctgttgtcc
     9301 cacaggtgac aggcgtggtg atcgaagtga cggataagaa aaatacgctg attaaaaaag
     9361 gtgaggtgct atttcgactg gacccgacgc gttatcaggc gcgggtggat cggctgatgg
     9421 cggatatcgt taccgcagaa cataaacagc gggcgttggg cgcagagtta gatgagatgg
     9481 cggcgaatac tcagcaggca aaggccacgc gggataaatt cgctaaagag tatcagcgtt
     9541 acgcacgcgg cagccaggcg aaagtaaacc cgttttcaga acgcgatatt gatgtggcgc
     9601 ggcaaaatta tctggcgcag gaagcctccg taaagtcatc ggcggcggaa caaaaacaga
     9661 tccagagcca gctggatagc ctggtgttgg gtgaacattc tcaaatcgcc agcctgaaag
     9721 cgcagctcgc ggaagcaaaa tataaccttg agcagacgat agtgcgtgcg ccgagcgatg
     9781 gttatgtgac ccaggtgctg attcgtccgg gtacctatgc cgcgtcgctg ccgctacgtc
     9841 cggtgatggt gtttataccc gatcagaaac gacaaattgt ggcgcagttc cgtcagaact
     9901 ccttgctgcg actggcccct ggcgacgatg cggaagtggt gtttaatgct ctgccaggta
     9961 aggtattcag cggcaagctg gcagccatta gtccagctgt tcccggcgga gcttatcagt
    10021 cgaccggcac cttacagacg ttaaacacag cgccgggttc agatggcgtt atcgcgacca
    10081 ttgaactgga tgagcacact gatttgagcg ctttaccaga cggtatttac gcccaggtgg
    10141 cggtctactc tgatcatttc agccatgtct cggtgatgcg caaagtgcta ttacgcatga
    10201 ccagctgggt gcattacctt tatctcgatc attaatcttc gctttccgcc gcttgttcag
    10261 gccgcagttg ctgcgactga gcaagctggt ggcgaatttc acgcagcatt tcatccagca
    10321 catactggac ccgccagggc aggtattccc gtttacggaa gatagccacc agatccagcc
    10381 accattcata cgggtgctga aagcagggca ccagcgtgcc gtcttgcaga tcgctctgca
    10441 cacttttgtc tggcgcaaac acaatcccca ggtgatttcg tgccagctcc agcgccgatt
    10501 gcgtgttatc gcaaacgtaa tttcccttca cgcgataatc ccgcacctct ttactcccgg
    10561 cgacgttgaa gcgccagata ttcgcgtcgt cgatcatcat cgagtcgatc aaaatacagg
    10621 agtgatgttc gagttcgtca ggacggctaa taggatgttt ctcaagatag cgctggctgg
    10681 cataagcggt tacggcatat tgtgtaataa aactggcaac cagcgattca tcttttggtg
    10741 gagcgtagct gattaaaaca tcacaatcat caggaaattc gacgccttca taaaaggcat
    10801 tacgctcaa
//
LOCUS       DADMGC010000132        10461 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.132, whole genome shotgun sequence.
ACCESSION   DADMGC010000132 DADMGC010000000
VERSION     DADMGC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.132"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="JNZ73_003390"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="JNZ73_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HAY8733554.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAEMVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1151..1237)
                     /locus_tag="JNZ73_003391"
     CDS             complement(1151..1237)
                     /locus_tag="JNZ73_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HAY8733555.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="JNZ73_003392"
     CDS             complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="JNZ73_003392"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAY8733556.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="JNZ73_003393"
     CDS             complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="JNZ73_003393"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HAY8733557.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="JNZ73_003394"
     CDS             complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="JNZ73_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HAY8733558.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     EMEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGCDKGHQWMEEQLVSICKR"
     gene            5389..5475
                     /gene="leuL"
                     /locus_tag="JNZ73_003395"
     CDS             5389..5475
                     /gene="leuL"
                     /locus_tag="JNZ73_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HAY8733559.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5568..7139
                     /gene="leuA"
                     /locus_tag="JNZ73_003396"
     CDS             5568..7139
                     /gene="leuA"
                     /locus_tag="JNZ73_003396"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAY8733560.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7139..8230
                     /gene="leuB"
                     /locus_tag="JNZ73_003397"
     CDS             7139..8230
                     /gene="leuB"
                     /locus_tag="JNZ73_003397"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAY8733561.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8233..9633
                     /gene="leuC"
                     /locus_tag="JNZ73_003398"
     CDS             8233..9633
                     /gene="leuC"
                     /locus_tag="JNZ73_003398"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAY8733562.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9644..10249
                     /gene="leuD"
                     /locus_tag="JNZ73_003399"
     CDS             9644..10249
                     /gene="leuD"
                     /locus_tag="JNZ73_003399"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAY8733563.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10298..>10461)
                     /locus_tag="JNZ73_003400"
     CDS             complement(10298..>10461)
                     /locus_tag="JNZ73_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8733564.1"
                     /translation="ATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVAL
                     FLTAKVKDV"
BASE COUNT         2736 a         2744 c         2561 g         2420 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg
       61 cggcttagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccatc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa
     1141 gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat ttcgcaaaaa
     1201 aagcccaacg tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat
     1261 tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa ttttcgccac
     1321 atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt
     1381 ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac
     1441 ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatg
     1501 cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta attgcttttc
     1561 gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg
     1621 atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc
     1681 aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat
     1741 aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc
     1801 atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg
     1861 cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg
     1921 atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa
     1981 gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag
     2041 cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc
     2101 tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac
     2161 gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa
     2221 ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc
     2281 ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc
     2341 gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac
     2401 tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc
     2461 gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc
     2521 gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt
     2581 gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa
     2641 atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatg
     2701 cccagtgcct gacgatgcgt tgccggaaac gcccccagcc ccatcaatga ggaaacaacg
     2761 ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc
     2821 ccaccgccta cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta
     2881 atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg
     2941 cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact
     3001 ggtcctgggc gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct
     3061 tccgtttgct taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg
     3121 cactcctgaa aagcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact
     3181 aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccctgga
     3241 cccgacgtta ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata
     3301 tgcaccgccg cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat
     3361 gcatcgtaaa tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga
     3421 tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact
     3481 gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca
     3541 gccagagaca taaatgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat
     3601 aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca
     3661 gcaatttctc tattttttta attaatccta aaatagagag tccacacatt cagaataaaa
     3721 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
     3781 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
     3841 acccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt aacggcagcg
     3901 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
     3961 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
     4021 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
     4081 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
     4141 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
     4201 taaattcagg acgatggaag tcttcataac taatcacaaa ctccatttcc tgataacgca
     4261 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
     4321 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
     4381 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
     4441 gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
     4501 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
     4561 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatgtttt
     4621 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
     4681 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     4741 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     4801 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     4861 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa
     4921 taatccataa tgtttttgtt gcgaaaacaa tctaatcata actaccgcga atactcaatc
     4981 atctacaaaa tggattaaat gtgcttattt aataattatt ttatatattt aatgcattaa
     5041 ttcttaacat taattgatca ataatattca ccaaatcaat atcaaaaaaa atcgcaaaac
     5101 atataattca atacaaatca ttaggatagg ttttgcaacg cgtgcatttt gtcccctttt
     5161 tcctcgttga ttagatgcaa aaatttatgc tgaaatatgt caaccgatga aaagcgtcgg
     5221 tagttaagca gaaattaata tcgcttactt taaccaccgt agcacaatta gctaatttta
     5281 cggatgcaga actcacgctg gcgggacgtt tttattgcgt cagggttgac atccgttttt
     5341 gtatccagta actctaaaag catatcgcat tcatctggag ctgatttaat gactcacatc
     5401 gttcgcttta tcggtctact actactaaac gcatcttctt tgcgcggtag acgagtgagc
     5461 ggcatccagc attaagccag cacgcagtca aacaaaaaac ccgcgccatt gcgcgggttt
     5521 ttttatgccc gaagcgaggc gctctaaaag agacaaggac ccaaaccatg agccagcaag
     5581 tcattatttt cgataccaca ttgcgcgacg gtgaacaggc gttacaggca agcttgagtg
     5641 tgaaagaaaa actgcaaatt gcgctggccc ttgagcgtat gggtgttgac gtgatggaag
     5701 tcggtttccc cgtctcttcg ccgggcgatt ttgaatcggt gcaaaccatc gcccgccagg
     5761 ttaaaaacag ccgcgtatgt gcgttagctc gctgcgtgga aaaagatatc gacgtggcgg
     5821 ccgaatccct gaaagtcgcc gaagccttcc gtattcatac ctttattgct acctccccaa
     5881 tgcacatcgc caccaagctg cgcagcaccc tggacgaggt gatcgaacgc gctatctata
     5941 tggtgaaacg cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa gatgccgggc
     6001 gtacacccat tgccgatctg gcgcgagtgg tcgaagcggc gattaatgcc ggtgccacca
     6061 ccatcaacat tccggacacc gtgggctaca ccatgccgtt tgagttcgcc ggaatcatca
     6121 gcggcctgta tgaacgcgta cctaacatcg acaaagccat tatttccgta catacccacg
     6181 acgatttagg cctggcggtc ggcaactcac tggcggcggt acatgccggt gcacgccagg
     6241 tggaaggtgc aatgaacggg atcggcgagc gtgccggtaa ctgttcgctg gaagaagtca
     6301 tcatggcgat taaagttcgt aaggatattc tcaacgtcca caccgccatt aatcaccagg
     6361 agatatggcg caccagccag ttagttagcc agatttgtaa tatgccgatc ccggcaaaca
     6421 aagccattgt tggcagcggc gcattcgctc actcctccgg tatccaccag gatggcgtgc
     6481 tgaaaaaccg cgaaaactac gaaatcatga caccagaatc tattggtctg aaccaaatcc
     6541 agctgaatct gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg gatgagatgg
     6601 ggtataaaga aagtgaatat aatttagaca atttgtacga tgctttcctg aagctggcgg
     6661 acaaaaaagg tcaggtgttt gattacgatc tggaggcgct ggccttcatc ggtaagcagc
     6721 aagaagagcc ggagcatttc cgtctggatt acttcagcgt gcagtctggc tctaacgata
     6781 tcgccaccgc cgccgtcaaa ctggcctgtg gcgaagaagt caaagcagaa gccgccaacg
     6841 gtaacggtcc ggtcgatgcc gtctatcagg caattaaccg catcactgac tataacgtcg
     6901 aactggtgaa atacagcctg accgccaaag gtcacggtaa agatgcgctg ggtcaggtgg
     6961 atattgtcgc caactacaac ggtcgccgct tccacggcgt cggcctggcc accgatattg
     7021 tcgagtcctc cgccaaagcc atggtgcacg tactgaacaa tatctggcgt gccgcagaag
     7081 tcgaaaaaga gttgcaacgc aaagctcaac acaacgaaaa caacaaggaa accgtgtgat
     7141 gtcgaagaat taccatattg ccgtattgcc gggagacggt attggtccgg aagtgatgac
     7201 ccaggcgctg aaagtgctga atgccgtgcg caaccgcttt gcgatgcgca tcaccaccag
     7261 ccattacgat gtaggcggcg cagccattga taaccacggg caaccactgc cgcctgcgac
     7321 ggttgaaggt tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg gcccgaagtg
     7381 ggaacattta ccaccagacc agcaaccaga acgcggcgcg ctgctgccgc tgcgtaagca
     7441 cttcaaatta ttcagcaacc tgcgcccggc aaaactgtat caggggctgg aagcattctg
     7501 tccgctgcgt gcagacattg ccgctaacgg cttcgacatc ctgtgcgtgc gcgaactgac
     7561 cggcggcatc tatttcggtc agccaaaagg ccgcgaaggt agcggacaat atgaaaaagc
     7621 gtttgatacc gaggtgtatc accgttttga gatcgagcgt attgcccgca tcgcgtttga
     7681 atctgctcgt aaacgtcgcc acaaagtcac ctcaatcgac aaagctaacg tgctgcaatc
     7741 ctctatttta tggcgtgaaa tcgtcaacga gatcgccacg gaatacccgg atgtcgaact
     7801 ggcgcatatg tacatcgaca acgccaccat gcagctgatt aaagatccat cacagtttga
     7861 cgttctgctg tgctccaacc tgtttggcga cattctgtct gacgagtgcg caatgatcac
     7921 tggctcgatg ggcatgttgc cttccgccag cctgaacgag caaggttttg gactgtatga
     7981 accggcgggc ggctcggcac cagatatcgc aggcaaaaac atcgccaacc cgattgcaca
     8041 aatcctgtcg ctggcactgc tgctgcgtta cagcctggat gccgatgatg cggcttccgc
     8101 cattgaacgc gccattaacc gtgcattaga agaaggcatt cgcaccgggg atttagcccg
     8161 tggcgctgcc gccgttagta ccgatgaaat gggcgatatc attgcccgct atgtggcaga
     8221 aggggtgtaa tcatggctaa aacgttatac gaaaaattat tcgacgctca cgtagtgtac
     8281 gaagccgaaa acgaaacccc gctgttatat atcgaccgcc acctggtgca tgaagtgacc
     8341 tcaccgcagg cgttcgatgg tctgcgcgcc cacggtcgcc cggtacgtca gccgggcaaa
     8401 accttcgcca ctatggatca caacgtctct acccagacca aagacattaa tgcctgtggt
     8461 gaaatggcgc gcatccagat gcaggagctg attaaaaact gcaaagaatt tggcgtcgag
     8521 ctgtatgacc tgaatcaccc gtatcagggg atcgtccacg taatggggcc ggaacagggc
     8581 gtcaccttgc cggggatgac cattgtctgc ggcgactcgc ataccgccac ccacggcgcg
     8641 tttggcgcac tggcctttgg tatcggcact tccgaagttg aacacgtact ggcaacacaa
     8701 accctgaaac agggccgtgc gaagaccatg aaaattgaag tccagggcaa agccgcgccg
     8761 ggcattaccg caaaagatat cgtgctggca attatcggta aaactggcag cgcaggcggc
     8821 accgggcatg tggtggagtt ttgcggcgaa gcaattcgtg atttaagcat ggaaggtcgt
     8881 atgaccctgt gcaatatggc gatcgaaatg ggcgcaaaag ccggtctggt tgctcctgac
     8941 gaaaccacct ttaactatgt caaaggccgt ctgcatgcgc cgaaaggcaa agatttcgac
     9001 gacgccgttg cctactggaa aaccctgcaa accgacgaag gcgcaacttt cgataccgtt
     9061 gtcactctgc aagcagaaga aatttcaccg caggtcacct ggggcaccaa tcccggccag
     9121 gtgatttccg tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc ggttgaacgt
     9181 gcgtcggcag aaaaagcgct ggcctatatg gggctgaaac cgggtattcc gctgaccgaa
     9241 gtggctatcg acaaagtgtt tatcggttcc tgtaccaact cgcgcattga agatttacgc
     9301 gcggcagcgg agatcgccaa agggcgaaaa gtcgcgccag gcgtgcaggc actggtggtt
     9361 cccggctctg gcccggtaaa agcccaggcg gaagcggaag gtctggataa aatatttatt
     9421 gaagccggtt ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat gaacaacgac
     9481 cgtctgaatc cgggcgaacg ttgtgcatcc accagcaacc gtaactttga aggccgccag
     9541 gggcgcggcg ggcgcacgca tctggtcagc ccggcaatgg ctgccgctgc tgctgtgacc
     9601 ggacatttcg ccgacattcg caacattaaa taaggagcac accatggcag agaaatttat
     9661 caaacacaca ggcctggtgg ttccgctgga tgccgccaat gtcgataccg atgcaatcat
     9721 cccgaaacag tttttgcaga aagtgacccg tacgggtttt ggcgcgcatc tgtttaacga
     9781 ctggcgtttt ctcgatgaaa aaggccaaca gccaaacccg gacttcgtgc tgaacttccc
     9841 gcagtatcag ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg gctcctcgcg
     9901 tgagcacgcg ccctgggcat tgaccgacta cggttttaaa gtggtgattg cgccgagttt
     9961 tgctgacatc ttctacggca atagctttaa caaccagctg ctgccggtga aattaagcga
    10021 tgcagaagtg gacgaactgt ttgcgctggt gaaagccaat ccggggatcc atttcgacgt
    10081 ggatctggaa gcgcaagagg tgaaagcggg agagaaaacc tatcgcttta ccatcgatgc
    10141 cttccgccgc cactgcatga tgaatggtct ggacagcatc ggcctgaccc tgcagcacga
    10201 cgacgcgatt gcgtcttatg aagcaaaaca acctgcgttt atgaattaga ccccttgccc
    10261 ggtcaaatga ccgggctttc cgctatcgtc cacgtcatca aacgtcttta acctttgcgg
    10321 ttaaaaataa tgcgacaaca gaaataatcg caattaccca gtagacagca aagtgccccc
    10381 aactttgtgc aattgctccc tgaataacac cagccagaat taccccggta gagatagtgt
    10441 tggtaaataa ggtggtagct g
//
LOCUS       DADMGC010000133        10413 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.133, whole genome shotgun sequence.
ACCESSION   DADMGC010000133 DADMGC010000000
VERSION     DADMGC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.133"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..182
                     /locus_tag="JNZ73_003401"
     CDS             <1..182
                     /locus_tag="JNZ73_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY8733565.1"
                     /translation="VSDKPLHGEIKLPGQANRFYEGAISEHLQIGIRAIDLLRAEGDR
                     LHSRKLRTFNEPPFR"
     gene            525..1241
                     /locus_tag="JNZ73_003402"
     CDS             525..1241
                     /locus_tag="JNZ73_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY8733566.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLDAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(1697..1772)
                     /locus_tag="JNZ73_003403"
     tRNA            complement(1697..1772)
                     /locus_tag="JNZ73_003403"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1737..1739),aa:Asn,seq:gtt)
     gene            complement(1873..3500)
                     /gene="yeeO"
                     /locus_tag="JNZ73_003404"
                     /pseudo
     CDS             complement(1873..3500)
                     /gene="yeeO"
                     /locus_tag="JNZ73_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            3505..3580
                     /locus_tag="JNZ73_003405"
     tRNA            3505..3580
                     /locus_tag="JNZ73_003405"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3538..3540,aa:Asn,seq:gtt)
     gene            complement(3618..4568)
                     /gene="cbl"
                     /locus_tag="JNZ73_003406"
     CDS             complement(3618..4568)
                     /gene="cbl"
                     /locus_tag="JNZ73_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HAY8733567.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQHNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(4670..5587)
                     /gene="nac"
                     /locus_tag="JNZ73_003407"
     CDS             complement(4670..5587)
                     /gene="nac"
                     /locus_tag="JNZ73_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HAY8733568.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            5914..5989
                     /locus_tag="JNZ73_003408"
     tRNA            5914..5989
                     /locus_tag="JNZ73_003408"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5947..5949,aa:Asn,seq:gtt)
     gene            complement(6045..6980)
                     /gene="ldtA"
                     /locus_tag="JNZ73_003409"
     CDS             complement(6045..6980)
                     /gene="ldtA"
                     /locus_tag="JNZ73_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY8733569.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(7042..8121)
                     /gene="cobT"
                     /locus_tag="JNZ73_003410"
     CDS             complement(7042..8121)
                     /gene="cobT"
                     /locus_tag="JNZ73_003410"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAY8733570.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(8133..8865)
                     /gene="cobS"
                     /locus_tag="JNZ73_003411"
                     /pseudo
     CDS             complement(8133..8865)
                     /gene="cobS"
                     /locus_tag="JNZ73_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
     gene            complement(8862..9407)
                     /gene="cobU"
                     /locus_tag="JNZ73_003412"
     CDS             complement(8862..9407)
                     /gene="cobU"
                     /locus_tag="JNZ73_003412"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HAY8733571.1"
                     /translation="MMILVTGGAWSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(10034..10297)
                     /locus_tag="JNZ73_003413"
                     /pseudo
     CDS             complement(10034..10297)
                     /locus_tag="JNZ73_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2961 a         2630 c         2212 g         2610 t
ORIGIN      
        1 gtgtttcaga taaaccgtta catggtgaga ttaagcttcc cggccaggct aaccgttttt
       61 atgaaggcgc tatttccgaa cacctgcaaa ttggcattcg ggcgatcgat ttgctgcgcg
      121 cagaaggcga ccgactgcat tcacgtaaat tacgaacctt taatgagccg ccgttccgat
      181 aataagttat gaaccttctt atcagaaata gatgtgaagg attaagtaag acatctggca
      241 aatagcctgc aataaacgcg ggctgcttgc ttccttgggc agatgcgagt tttattatcg
      301 tcttaatgat ttccacatat taaaagcaag tatgctttca aaacacaatt ataaaaaatc
      361 ccgccaacaa cattagtttt tataaaatta aatataagat tatggcttta gaatattttt
      421 atttctaata gacgagattt ttcctgttat gatataatat gccgaattaa cacatgttaa
      481 cgatttacca gtaatgtaaa taaattttcg aggagatcat tccagtggga cgtaaatggg
      541 ccaatattgt tgctaaaaaa acggctaaag acggtgcaac gtctaaaatt tatgcaaaat
      601 tcggtgtaga aatctatgct gctgccaaac aaggtgaacc cgatccagaa ttaaacacat
      661 ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt tccaaagcac gttattgata
      721 aagcaattga taaagccaaa ggcggcggag atgaaacgtt cgtacaggga cgttatgaag
      781 gctttggtcc taatggctca atgattattg ctgagacatt gacttcaaat gttaaccgta
      841 cgattgctaa cgttcgcaca attttcaata aaaaaggcgg caatatcgga gcggcaggtt
      901 ctgtcagcta tatgtttgac aatacgggtg tgattgtatt taaaggaaca gaccctgacc
      961 atatttttga aattttgctt gacgctgaag ttgatgttcg tgatgtaact gaagaagaag
     1021 gtaatattgt tatttatact gaacctgctg accttcataa aggaatcgcg gcgctaaaag
     1081 cagctggaat cactgagttc tcaacaacag aattagaaat gattgctcaa tctgaagttg
     1141 agctttcccc agaagattta gaaatctttg aagggcttgt tgatgccctt gaagatgacg
     1201 acgatgtaca aaaagtttat cataacgtcg caaatctcta attatatttt aaagaaatct
     1261 gtctttatgg cagatttctt taatctcata taattcttat aaaaaatata atgtttaact
     1321 cgtcatattg cttataaaaa atccccgttc ccagagaaat aatatttatt aaaatggcag
     1381 gtcttctttt tctgattacc gaagacaaag tggcaattac gtatagtttc ccgataaaga
     1441 cgcgacagct acatcccgca taaggcattt ttctctttat ctttgtacgg tacttcatgg
     1501 aacagaattt ttgaccttgt gaatcgtgat gtctgttggg gaggggcaat ttgctcactg
     1561 aagcgtgaga ctcgattaag cgcacgaaac acagaaatca aaaaacccgg ccactttttt
     1621 acaaagtggc cgggtaaaaa taatttttat tttttaactg tcctgagtct catagagatg
     1681 tctcaaaact aaaatttggc tcctctgact ggactcgaac cagtgacata cggattaaca
     1741 gtccgccgtt ctaccgactg aactacagag gaatcgtgag aacgaggcga atattagcga
     1801 tgcccaccca caatgtcaaa gcctgttttt taaatttgaa atcgtttgct gaaataatct
     1861 gcattttgtc gtttattccg acacaactgg ctttttttca cacttttgcg gctcggatcg
     1921 agggtatttc cacagccaac gtccagtaac cattcgccag taaaacagca cggcccgcac
     1981 agcccagtag gcaaacattc ccatccagac accaaccaca ccccagccaa gcatgattcc
     2041 ctgcacataa ccgactacaa cccggcaacc ccacatgctc aacatagaaa cccacatggc
     2101 gtaacgggca tcacgagcac ccttaaatcc agcgggtagc acccatgagg cggaccaaat
     2161 aggcataaat aaagcattta gctaaatcag aatcacaacg acgtgtttaa cctgtggatc
     2221 ctgggtgtaa aacgatgcca taaccccggc aaagggagcc gttagccagg cgatggccgt
     2281 taatccaaga gtggacagcc agaacacatg ccgcaactga atctctgctt gcgctatctg
     2341 ccctaccccc aaccttcggc ctgtaatgat cgtagaagca gaaccgagcg cacttccggg
     2401 taagttgata agagccgcaa ttgagaacgc gataaaattt ccggcaataa cgctggtccc
     2461 catcccggca acgaacattt gggttaataa ccgaccactg gtaaataaca ctgattcgac
     2521 actcgctgga ataccaatcc ccatgacttc ccagataatg ctaaaattca gcggtttaaa
     2581 atagctcttt aacgaaatcc ttagcgcagg attaaaacca atcgccagca cccacaaaat
     2641 tgcaactgcg ccaatataac gagaaatggt taaacccaac ccagccccga ccaggagaaa
     2701 aggccgtaaa tcaatatgcc gctaataata atattaagaa tattcaggct accgttaatc
     2761 agtagcggta ttttcgtatt ccctgcacca cgaagtgccc cgctaccaat aagagtgatg
     2821 gcagcggctg gataactgag tacggtcagc tccagataag tcaacgccag tgctttaact
     2881 tctgtcgtgg catcacccgc gacgaaatca ataatttgtt cgccaaaatg atgaattagc
     2941 gttgccaaca gtacggcaaa caacgtcatg atcaccaatg actgccgcgt cgccaccctc
     3001 gctcgtcgtc gatcccgctt accgagacta aatgctacaa cgacagtagt accaagatcg
     3061 atagctgcaa aaaaagccat aatgaccata ttgaagctgt ccgccaaccc cacgccggcc
     3121 atcgcatctt ttcccagcca gctgaccaga aaagtgctca ggccccccat caacaggaca
     3181 cagacgttct ccatgaagat aggaacagca agcggggtta tctcgcgcca gaacaacact
     3241 ttgtagctct tgcgtttaga gtgccagcga gtgccgtgaa caacctggcg taaagcagag
     3301 gagatattca aagccgacct taattgcaga aagtgaaacc acatttcaaa tgatgaggga
     3361 gaatcagcaa agctgcaaag attttcgcca acaaattgtc tgcaaatgca acaaactgtt
     3421 gatagaaacg gcaaacagtt gggaaattta aaaatcgggt ttgacaaaat atttttcgcc
     3481 gttaagatgt gcctcaacag cgattcctct gtagttcagt cggtagaacg gcggactgtt
     3541 aatccgtatg tcactggttc gagtccagtc agaggagcca aatttctgaa aagcccgctt
     3601 ttatagcggg ctttttgcta tatctgataa tcaatttcct cttcactgtt ttccatcacc
     3661 tggcgcttga tatcctcaac tgacagtcct gcattacaaa gttccagaaa gcgccaaaca
     3721 tagttatgct gaagttgtcc tcgcttcagt cccaaccaga cagtattagc atcgaaaaga
     3781 tgccgcgtat ccaggcggat taaattctct tcctcttgtt cgccgctgga ttgttcggca
     3841 actaatccga tcccaagccc aagagcaaca taggttttaa tgacatcaga atcctgtgca
     3901 cttaatacaa tatctgccgg caaacctttg cgggtaaatg cgtcatcagt gcgtgagcgc
     3961 cccgtaattc cctgtcggta agtaattaac ggccacttcg ctattgattc cagcgtcaat
     4021 ggtgtaattt gcgttaaggg atgatcgagt ggaacaagca aactatggtg ccaacaaaac
     4081 cacgggaagg cgacgagctg cgggtcatta ctcaaacgct cgctggcgat accaatatca
     4141 gcttcgccat tttgcaacaa cgtcgcaatt tcctgtggcg aaccctggat tagctcgagc
     4201 cgaacctccg ggaaaagttc gcgaaaagct ttaatgacct ctggcaagct ataacgtgcc
     4261 tgagtatgcg tcgttgcaat agtgagaaca ccagacgtat cgttggtaaa caggtctgca
     4321 agccgacgaa cattactggc ttcattcaga atacgttctg caatgaccag taatgctttg
     4381 cccggttcag tcatccccag cagtcgctta cctcgtcgaa caaatatttc gatgccaagt
     4441 tcatcctcca gttcccgaat atgacggcta acgcctgact gtgaggtaaa aagcatattc
     4501 gcaacctctg tcaggttgta atcctgacgt gcagcctcgc ggattatctt tagttgttgg
     4561 aaattcacgg taaactccgg gcagttcaga tatcccgtta ttgttaaagt ctaatgcccg
     4621 gcataacaaa taataaaaac ccgcatctta ttccatcccg atataacgct tagctcacca
     4681 attgccactg ccttttttcc atcactggag aactaatcac tgacattaac aactctttca
     4741 ctgcctgtgc ctgtggtgac aagtttgctc tggcgggtaa atttaaggac aaagagagac
     4801 tcattgaagg agtggtaatg cgtgacatcc acccatttac tgcgccacat aacgaacgcg
     4861 cagccgattc aggtaatact gcaacgccca tgccgctggc aatcgctgcg gtaagcgtgg
     4921 caatagactc aatttcacca atgacttttg ccgtgagccg ccgtagggaa aaagcctcat
     4981 caacacgaag tctaacagcg ctgtaatcac tggggagaaa aaggttcatt tgcgcaatag
     5041 cattaacatc aacgttttgc cccgggcaat cttgagttcc taccaggaaa agatcttctt
     5101 tcagcaaagc ctgactggat accccagcca caggggaatg ctcataaatc accgccatat
     5161 cgagttggtg atttatcagt ttttcgttaa gcactgcgcc actattttca tgaagataga
     5221 taacgacctc cgggaattca gcgcgaaccg cctgtaataa gggcatggtg atggatgacg
     5281 cagcggttcc tggcgcaaag ccaatcgaga cttgccccga taatgactga ccaacgttat
     5341 gcaccgccag ttgggcctgc tcacactgac gtaaaatggc ccgtgcatgg gtatagagaa
     5401 tttttccggc gtctgttggc gtaacgcccc gttttgtgcg aatcaaaagc tgttgattta
     5461 actcaccttc cagtatggca acctgctggc tgagcgctgg ttgtgcgata tgcaatactt
     5521 cagcagcctg ggtcaggcta ccaatatcta caatttttac gaagtatttc aggcgtctga
     5581 agttcatgtt gcctccggtt tttaagaatc ggcccaagtg ccgccattac ttacaaccag
     5641 tatgcaagat gcctgccagt tctattttgg cgttgatgcg caagctaacc aactgtcaaa
     5701 taagagatta tgatagattc gtcattggcc ctttttatca gcagtcgcat tcacctgccc
     5761 tataaaagga gggtatgcac cacgatggtt cattacccaa taagattgaa agctcaccac
     5821 tttgttgaaa ttggcagcaa acaaacaaaa aaatgcattt caccctttga catcaccatg
     5881 cactgccatt aatatgcgcc ccgttcacac gattcctctg tagttcagtc ggtagaacgg
     5941 cggactgtta atccgtatgt cactggttcg agtccagtca gaggagccaa attcaaaaaa
     6001 gcctgctttc gagcaggctt tttactttct aattaccaac gctcttaaaa catctgtctt
     6061 gaaccagaac taatttgcac aggcattccc gatcgatgtt gcaacgcagc atttgcgcga
     6121 tttacatcaa cttcttgccc gttgataaac gcccgcaaag atggggttac cggcaatggc
     6181 acttttcggt cagactcata ttctgcacga ttgcgtgaca gcggctcatg aacttccagc
     6241 cactttgaac catccggttc cgttgtgtat ttcactggct gatcaataat ttgtacacgt
     6301 gtgcctacag gaacattatc aaacagatat ttgatatcgt cattacgcag acgaatacag
     6361 ccctgactta cacggagccc aataccaaaa ttggcattgg taccgtggat ggcatacaac
     6421 ctgccaatat aaatcgcgta cagccccatg gggttatcgg gccccgcagg aacaaatgcg
     6481 ggcaaactct cccctcgttt cgcatattcg cgccgggtgt tcggcgttgg cgtccaggtt
     6541 ggtgcttctt gtttacgttc aacggtagtc acccagttac gcggggtttc tcgcccagcc
     6601 tggccgatac caataggaaa gacttccact gtattactgt ctggcggata gtaataaaga
     6661 cgcatttcag cgacgttaac cacaatccct ttacgaacag tggcgggcaa aatcagttgc
     6721 tgcggaatgg tgagttgtga gccaggcttc ggtaaaaaaa catcagcgcc cggattcgct
     6781 tccagcatat tacttaaccc ttgcccgtat tgtgcggcaa aagtctccag cggctgagta
     6841 ttgtgatcag gaacagttac agtaagcgac tgccccacta aacggctacc ctctggaggt
     6901 aatggataag ttaccgccag gctattttgg ctggcaaaaa gcagagcaaa tgagcaaaga
     6961 atatttacac gacgcatcat gtccctttcc taagtcgcga atactatccg ataagtatag
     7021 cttttatcag acttttcgtt tttagctgtt caaatcagaa gtcgtattcc ccggtagaac
     7081 aatattactg gcagcaagtt cgcccatgtt gttgtatatc gcacaagcag cttcgatgat
     7141 ggacatcgcc agagctgcac cactcccctc acctaaacgc atatccatat tgagataagg
     7201 ctccagcccc aaatgcgaga gcgctatacg cgcgcctttt tctgccgaca agtgagaagg
     7261 aatgagatac ggtttgattg caggagacat ctggcaggct gcgagcgcag cagcataaga
     7321 aagaaatcca tccagcaaca cgggtaaacc gcaggaagca gcacctaaca tcactccagc
     7381 tattccgacc aaatcaaatc cacccacttt tgccaggaca tcaacaccat cctgaggatt
     7441 tggttgattc aacgtaatcg cccgacgcac aacatcaatt ttattagcca gtttatctgt
     7501 cggcaggttt gcgccaatcc caaccacttc ttcaggatcc cggccagtga ttgtgctgac
     7561 tattgccgct gccggtgtcg tgtttgccat ccccagttca cctacaccaa acagcgtgac
     7621 accgtttttc gccagctccc gcgtataaca tatgacgtcc aaaagcaact tttcagcctg
     7681 acggcgactc attgccggag ctgaagcaat attgccgcta cctcgtgcga cacgcatgtt
     7741 gataagcccg gggataggct cagcagtatc aataccaaca tcaattacgt ggacgttagc
     7801 gcccgcttgt gctgccagca cacacacgcc ggttgttcca cgggtcatat tttcagcctg
     7861 tatggctgtc acttcttttg gagaaatagc gaccccttcc tcccagacgc cgtgatcggc
     7921 acacataacc agtaccgctt ttttgcccac atgcggtatg ccattcaccc ccggcattcc
     7981 tgccagttgt atggcaagcg cctccagctt tcctaggcta ccaacaggtt tgagtaaccc
     8041 gtcaacatgc cgttgtgcac gcgacatagc ggcagaattg atggctggga tcgtattcag
     8101 taaatcggca attatttgca tgtcatgtct ctttatagca gtgccagcag gaagaccagt
     8161 tcaccaagct ctatggctgc tcccagcgta tcgcccgttt gcccgcccag cgtacgtttg
     8221 agaagctgac cgaggatgaa aatcgccacc atcgtcacca ccatagcagc cacaccatgc
     8281 atacccagca ataatacggc agcaaaaata gcggctaagc cgagtgtgac gcaggtttgt
     8341 cgcgccaatg aacacattgc caagcccttc ttcccgcgca taacgatgac gatacatcaa
     8401 taatgcggca gtcccgcggc tgaccgcaca tgctgccgcc agcgatgcaa ggatcggttc
     8461 gccacgcaga gccaactcgc ttaacaccag aatctttgcc agtaccacaa aaatcaatgc
     8521 cagaccgccg tgggtgccta aacggctatc acgcatgatt tccaacatgc gatcgcggct
     8581 acgtgcagaa aatacgccat cacaggtatc agcgaggccg tccagatgga attccccggt
     8641 catcagcgcc agcacaagta cgctaaacag tgccgccagt ggtgcgccac accatgcctg
     8701 cagcaccatg aagaccagcc cgctaatcgc gccaagcaat aatccaatca aaggaaaagt
     8761 aataataccg cgagaataat gctcgaaatc cagtccctgg gaccagcgac gcggtacagg
     8821 cagacgcgta ataaatgaga gcatcgccca aaataatttg ctcatttgat ttttactcca
     8881 atacccgaaa ccaccagcca tacttcattt gccgcagcgg ccaactgctg atttacccgc
     8941 ccggcaatat cacgaaaatg tcgtgccaga cgactctccg gcacaatccc cattcccact
     9001 tcgttagtca ctaatacaac ctttgcgggg caacgttggc aggcagcaat caacgactga
     9061 atctcagcat taatcgcctg ttccatcgcc tgataatccc attcatcagg gtctttatcg
     9121 ccgccataat caaacaacag attagtcacc attgtggtaa cgcattccag caacacagcc
     9181 tcattcgggt taatgtctgc atgaattaat tcatcaagat gttgccagcg ctccactgtg
     9241 cgccagtgct ccgggcggct ttgccgatga tgttctatcc gtgcagccat ctcatcatca
     9301 aggatttgcg aggtagcgat atacagaacc tgtgaagagt ccccaataag cacctctgca
     9361 tggcgactct tcccgctcca tgcgcctccc gtcaccaaaa tcatcatacc gactcctgat
     9421 tcaaatgtat gatggtatac attgaacaga gcaatggatc ccccaccgtt gctgaccgca
     9481 aaacatttac gcgattaata ctgcgcgtaa tataattaaa aatccagcat tctcaatcca
     9541 ttacgaaata agatggcatt acggaatagc cagactttct cgtgttaacg tccgacaaaa
     9601 tgcgtcattg acagcggttt ctgtggattg acacatttca ttcagataat gaattaatgc
     9661 tactgccgga ccaaccagca aacgggcatt gtgttctgaa atccaggcta ttgattcaac
     9721 gtcagcgaca gatctgcgct gtaaaagaac ttgtaaaaca acgcaataga attatccgga
     9781 atggtggcga ctatgcacta gaacaccaca atttcgctct ctcggtaagg cactgtcaca
     9841 gcattcaaca gaatgtgact tgccagattt attagcgcca ccagatgttt aaccgggtaa
     9901 ccacgaccca gtcggggata tattccatct tctcgctgac atcatcaaga ataaaaaggt
     9961 ttatcacact cagagcaacg ttcttcagca ggcgggataa ttcttcaaca tttaaaagat
    10021 gtgttattaa aggcatctca aattggacag ggctgccggt tttctgttgc aacaccgttg
    10081 ctctgcttga gacagaactg ccatatgcag catctgatac tttgagtttg accaaatcac
    10141 agccccgtag cttactgtcc aaagccatgt tgaacagagc tagatcgcgt gttttacctt
    10201 ccaactcaag tctaatccgg atcccccaga tatgagatat ctgaagtggc cttttttgac
    10261 cgatggtacg gtctttattc cacggtgacg ttttcatatt caaatctcct gcaatgtggg
    10321 agatttgagt atggttgtct ctcaagagcg aaaataagtc aactatcact agttgttgcg
    10381 catgtacgta ttctgtcaat taattattga caa
//
LOCUS       DADMGC010000134        10256 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.134, whole genome shotgun sequence.
ACCESSION   DADMGC010000134 DADMGC010000000
VERSION     DADMGC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.134"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            80..883
                     /gene="hpaI"
                     /locus_tag="JNZ73_003414"
     CDS             80..883
                     /gene="hpaI"
                     /locus_tag="JNZ73_003414"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HAY8733572.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            905..2260
                     /gene="hpaX"
                     /locus_tag="JNZ73_003415"
     CDS             905..2260
                     /gene="hpaX"
                     /locus_tag="JNZ73_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HAY8733573.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSGIIMASMGSFTAMTIFWTTQDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            2270..3160
                     /gene="hpaA"
                     /locus_tag="JNZ73_003416"
     CDS             2270..3160
                     /gene="hpaA"
                     /locus_tag="JNZ73_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HAY8733574.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFTRLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSPEGMEHTLAALNHYWPIISLEFQQNKPG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            3268..4124
                     /locus_tag="JNZ73_003417"
                     /pseudo
     CDS             3268..4124
                     /locus_tag="JNZ73_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            4351..5909
                     /gene="hpaB"
                     /locus_tag="JNZ73_003418"
                     /pseudo
     CDS             4351..5909
                     /gene="hpaB"
                     /locus_tag="JNZ73_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            5927..6439
                     /locus_tag="JNZ73_003419"
     CDS             5927..6439
                     /locus_tag="JNZ73_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HAY8733575.1"
                     /translation="MQLDEQRLRFHDAMVSLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            6832..8982
                     /gene="btsT"
                     /locus_tag="JNZ73_003420"
     CDS             6832..8982
                     /gene="btsT"
                     /locus_tag="JNZ73_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HAY8733576.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            9032..9235
                     /locus_tag="JNZ73_003421"
     CDS             9032..9235
                     /locus_tag="JNZ73_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY8733577.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            9246..10202
                     /gene="yjiA"
                     /locus_tag="JNZ73_003422"
     CDS             9246..10202
                     /gene="yjiA"
                     /locus_tag="JNZ73_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HAY8733578.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
BASE COUNT         2445 a         2670 c         2650 g         2491 t
ORIGIN      
        1 agccccgaaa aagagccagt cagttgctta actgactggc attactccat tagctgccgc
       61 tttgtttaag gagagaacga tggaaaacag ttttaaagcg gcgctgaaag caggccgtcc
      121 gcagattgga ttatggctgg ggctgagcag cagctacagc gcggagttac tggccggacc
      181 aggattcgac tggttattga tcgacggtga acacgcgccg aataacgtgc aaaccgtgct
      241 cacccaacta caggcgattg cgccctaccc cagccagcca gtggtacgcc catcgtggaa
      301 cgatccggtg caaatcaaac aactgctgga cgtaggcaca caaaccttac tggtgccgat
      361 ggtacaaaac gccgacgaag cccgtaaagc ggtacgcgcc acccgttatc cccccgccgg
      421 tattcgcggt gtgggcagtg cgctggctcg cgcctcgcgc tggaatcgca ttcctgatta
      481 cctgcaaaaa gccaacgatc aaatgtgcgt gctggtgcag atcgaaacgc gtgaggcaat
      541 gaagaactta ccgcagattc tggacgtgga aggggtcgac ggcgtgttta tcggcccggc
      601 ggatctgagc gccgatatgg gttatgccgg taatccgcag cacccggaag tacaggccgc
      661 cattgagcag gcgatcgtgc agatccgcga agcgggcaaa gcgccgggga tcctgatcgc
      721 caatgagcaa ctggcaaaac gctatctgga actgggcgcg ctgtttgtcg ccgtcggcgt
      781 tgacaccacc ctgctcgccc gcgccgccga agcgctggca gcacggtttg gtaatagttc
      841 ttctatttcg atggcgaagc aaaacaacaa ctctgtctac taaacgtacg atgtggagcg
      901 catcatgacg acctctaccc tgcaatataa agataataaa actgttgaaa ttgaacatcg
      961 tgtcattaat aaattatttc gtcgcctaat catttttctt tttattctgt ttgttttttc
     1021 gtttcttgat cgcatcaata taggatttgc cggactgaca atgggcaagg atcttggcct
     1081 gacgtccaca atgtttggcc tcgccgcgac actcttttat gtcacgtacg tcatgtgcgg
     1141 gatccccagt aatatgatgt tagcgaaaat tggcgctcgt cgatggatcg ctgggatcat
     1201 ggtggtctgg ggaattgctt ccacctgcac aatgtttgca acaagccccg aaacactcta
     1261 tctactgcgt atgctggttg gtattgctga ggcgggtttt ttacctggta ttttggttta
     1321 tctgacctgg tggttcccgg catatcatcg ggccagagcc aatgccttat ttatgatagc
     1381 gatgccagtc actatgatgc tgggattgat cctttcaggc tacattctgg ccttggatgg
     1441 actgtggaat ctaaaaggct ggcaatggct ctttttgctg gaaggtctgc catcggtcat
     1501 tcttggtgtc gtcacctggt tttatctcaa cgacacacca gaccaggcga cctggcttga
     1561 tgaaaacgaa aaacaggctt taaaaacgat gattctccgt gaacaagaga gtgcatttgc
     1621 ccatgcatct gttccacgct caaccttacg tgaagtcctg accccagcca tcatgctata
     1681 tacacttgct tatttttgcc tgactaatac tctgagtgcg attaatatct ggacgccgca
     1741 aatcctacag agtttcaata ctggcagtag caacatcatg attggtttgc tggcggccat
     1801 cccccaattc tgcacaattt ttggcatgat ctggtggagt cgtcgttccg accgtcttaa
     1861 agaacgcaaa aagcatacga ttcttcccta cctgtttgcc gctgccggat ggttactcgc
     1921 ttctgcgaca gatcacagcc tcattcaact gagcggcatc attatggcct caatgggatc
     1981 atttaccgcg atgactatct tttggacaac acaggatcaa gttataagct tgcaatcgcg
     2041 tgccgttgcg atggctgtca tcaatgctgt cggcaatgta ggttcagcag tcagtccatt
     2101 acttatcggt attttgaaag atgcaaccgg cagttttagc tcaggtttgt ggtttgtagc
     2161 aggattgctg gtactgggcg cgttagtcat gacccgaatc cctatgggga gtcgggaaca
     2221 ctctctttca tctgaactta tcgcaccaaa accacaataa ggagtcatga tgtccagtga
     2281 agctatcttt gaacatggtt atgaaaatat tgatttggtc cgggaatatg atgcccgcta
     2341 cgctggtgaa gatgtgcatt atgaaacatt tacccggtta gcgaatttct ttggacgcga
     2401 tatgcgccct cactggcacg atagatattt tcaactccat tatctggtca ctggacgcat
     2461 tactttacaa ctggatgagc acttctattc tgtgcgcgca ccgctttttg tactaacacc
     2521 accatcggtg ccacatactt tctttactga tcctgatagc gatgggcatg ttctgaccgt
     2581 acgtcaggag ttaatttggc ccctggtaga aaagctctgg cctggtagtg gagatgccgc
     2641 aatgctacaa ggtatctgtc tgtcaccgga gggaatggaa catacactgg cggcattaaa
     2701 tcattattgg ccgataatat ccctggagtt tcaacaaaat aagccaggac gggaaattct
     2761 attgcaatca ctggcgcaaa gtatttttac aatcctactg cgtgaagcac caccaaatga
     2821 tatttcaaca tgcagcgtgc gcggcgagat gtgtttattc cagaagttta atcggctgat
     2881 tgacgaaaat tatcgccaac atttaatggt ccctgaatat gcaacaatgc tcggtatgag
     2941 tgaatcacgg cttaccgagc tgtgccgacg ttttgccaat caatcgccga agagattgat
     3001 tttcgaacga gtgagccggg aagccagacg gcttctgctc tatagcgagc aaagcatcaa
     3061 ccagatagcc cttgatcttg gctacaaaga tcccgcctat ttcgcccgtt ttttcaaccg
     3121 aatgatgggt tgctcaccaa cacaattccg tggtcgctga ccatttatca acctgagaga
     3181 ctcgacatga aaatatcttt acgctcacta tttgtggcgt gcctgattgc gccagtggca
     3241 ttcacctacg cctccgaacc gcaataaatg aaaattgtac tacttggaac gaaaggtggc
     3301 ccttctttat taaaagctga ccgtttaccg atggcaacgg cacttattat tggcaataaa
     3361 acctggttaa ttgatgctgg ttacggggcc agcttacaac tggtaaaaaa taatattcca
     3421 ctgcgccaga ttgataatat tttactcacc catttacaca gtgatcatat tctggactat
     3481 ccatcagtct taatgaatgc atgggctagc ggtctgcagg acaaaactat tcatgtattc
     3541 ggtccgccag gcacccgagc catgaccgct gccagttggc aggtcttcga tcgggacatc
     3601 acactgcgta tggaagaaga aggaaaaccc gatcctcgca aactcgttac agctcaagat
     3661 gttggtgaag gcgtagttta taaagatgat accgtgaccg tgactgcatt aaaggtccct
     3721 cattctcctt tccctgacgg tgaggctttt gcttatcgct tcgacacaca aggcaagcga
     3781 attgtcttct caggagatac ctcgtacttt cccccactgg caactttctc agaaggcgct
     3841 gatatacttg tgcatgaggc cgtacatgag ccctccgtcg ctaagctggc agacagtatt
     3901 ggcaatggta aaacactggc aaaagccatt gcttctcacc atacgactat tgaggatgta
     3961 ggcaaaattg ccaatgaagc aaaagtgaaa aaactcatcc ttagccacct ggtaccaatc
     4021 accgttccgg atgaaacatg gaagcaagaa gcacagaaaa ctattcagtc cctatcgtcg
     4081 ttggtcatga aaatatgacg attaccattc cattcgcccc ataaaaaatt ccgtccccgt
     4141 tagcccttgt tagcggggaa tattatgatt tgccttactg taagttgatc acgatcgcag
     4201 tttactattt aactccacct tgctgtcgaa aagtaccaac gttcgcctga accgtctctt
     4261 cccgacaacc gcgttcaacg cttcaattaa ataacaaaaa caacacacaa aattaacaat
     4321 cgtattccga ttaatactgt agaggtcgac atgaaaccag aagatttccg cgccagtacc
     4381 caacgtcctt tcaccgggga agagtatctg aaaagcctgc aggatggtcg cgagatctat
     4441 atctatggcg agcgagtgaa agacgtcacc actcatccgg catttcgtaa tgcggcagcg
     4501 tctgttgccc aactgtacga cgcgctgcac aaaccggaga tgcaggactc tctgtgttgg
     4561 aacaccgaca ccggcagcgg cggctatacc cataaattct tccgcgtggc gaaaagtgcc
     4621 gacgacctgc gccagcaacg cgacgccatc gctgaatggt cacgcctgag ctatggctgg
     4681 atgggccgta ccccagacta caaagccgct ttcggttgcg cactgggcgc gaatccgggc
     4741 ttttacggtc agttcgagca gaacgcccgt aactggtata cccgtattca ggaaactggc
     4801 ctctacttta accacgcgat tgttaaccca ccgatcgtca tttgccgacc gataaagtga
     4861 aagacgttta catcaagctg gaaaaagaga ctgacgccgg gattatcgtc agcggtgcga
     4921 aagtggttgc caccaactcg gcgctgactc actacaacat gattggcttc ggctcggcac
     4981 aagtgatggg cgaaaacccg gacttcgcac tgatgttcgt tgcgccaatg gatgccgatg
     5041 gcgtgaaatt aatctcccgc gcctcttatg agatggtcgc gggtgctacc ggctcgccat
     5101 acgactaccc gctctccagc cgcttcgatg agaacgatgc gattctggtg atggataacg
     5161 tactgatccc atgggaaaac gtgctgatct accgcgattt tgatcgctgc cgtcgctgga
     5221 cgatggaagg cggtttcgcc cgtatgtatc cgctgcaagc ctgtgtgcgc ctggcagtga
     5281 aactcgactt cattacggca ctgctgaaaa aatcactcga atgtaccggc accctggagt
     5341 tccgtggtgt gcaggccgat ctcggtgaag tggtggcgtg gcgcaacacc ttctgggcat
     5401 tgagtgactc gatgtgttca gaagcgacgc cgtgggtcaa cggggcttat ttaccggatc
     5461 atgccgcact gcaaacctat cgcgtactgg caccaatggc ctacgcgaag atcaaaaaca
     5521 ttatagaacg caacgttacc agtggcctga tctacctccc ttccagtgcc cgtgacctga
     5581 acaatccgca gatcgaccag tatctggcga agtatgtgcg cggttcgaac ggtatggatc
     5641 acgtccagcg catcaagatc ctcaaactga tgtgggacgc cattggcagc gaatttggtg
     5701 gtcgtcacga gctgtatgaa atcaactact ccggtagcca ggatgagatt cgcctgcaat
     5761 gtctgcgcca ggcacaaagc tccggcaata tggacaagat gatggcgatg gttgatcgct
     5821 gcctgtcgga atacgaccag aacggctgga ctgtgccgca cctgcacaac aacgacgata
     5881 tcaacatgct ggataagctg ctgaaataac gcagcaggag gttaagatgc aattagatga
     5941 acaacgcctg cgctttcatg acgcgatggt cagcctgtcg gcagcggtaa atattatcac
     6001 caccgagggc gacgccggac aatgcgggat tacggcaacg gctgtctgct cggtcacgga
     6061 tacaccaccg tcgctgatgg tgtgcattaa cgccaacagt gcgatgaacc cggtttttca
     6121 gggcaacggc aagttgtgcg tcaacgtcct caaccatgag caggaactga tggcgcgcca
     6181 cttcgcgggc atgacaggca tggcgatgga agagcgtttt agcctctcat gctggcaaaa
     6241 aggtccgctg gcgcagccgg tgctaaaagg ttcgctggcc agtcttgaag gagagatccg
     6301 cgatgtgcag gcgattggca cacatctggt gtatctggtg gagattaaaa acatcatcct
     6361 cagtgcagaa ggtcacggac ttatttactt taaacgccgt ttccatccgg tgatgctgga
     6421 aatggaagct gcgatttaag ctccagcccc ttcacgcatt cgtcgaaggg gcatttttat
     6481 tgtgatctca atcacatatc tacccgatga ataattcttg cccaaccgct caccgccatt
     6541 cctgaccgct tacatcccta aaataaccac tcagttattt accttacttt acgcgcacgt
     6601 atctctggca acatcactgc aggatagcgg tcaatttacc tcctcaaacg caacgcaaac
     6661 ctagaacggc ttcggcctat gattaacaaa ttactctatg gattttggtt tgcaggaagg
     6721 cagcaagtga gtgaatcccc gggagcttac aaaagtaagt gtcagaggtg aacgaacgca
     6781 gctgccgcac ctgtaagcca aaagacgacg agtaaaatgc caggttttac tatggatacg
     6841 aaaaaactat tcaagcacat accctgggtg attctcggaa tcatcggtgc attctgcctc
     6901 gcggtagttg cattacgtcg gggggagcac gtcagcgccc tgtggatcgt ggtcgcctct
     6961 gtgtcggtgt atctggtggc gtatcgctac tacagtctgt acatcgccca gaaggtgatg
     7021 aaactcgacc ccacgcgcgc gacgcctgcg gttattaaca acgacggtct gaactacgtt
     7081 ccgaccaacc gttacgtgtt gtttggtcac cacttcgccg ctatcgccgg tgctggtccg
     7141 ctggtgggac cggttctcgc cgcgcagatg ggctacctgc ctggcacgct gtggctgctg
     7201 gcgggggtag tgctggccgg tgcggtacag gactttatgg tgctgtttat ctcctctcgc
     7261 cgtaacggcg catctctcgg tgagatgatc aaagaagaga tggggccagt accggggact
     7321 atcgcgctgt ttggctgttt cttaatcatg atcatcatcc tcgccgttct ggcgctgatt
     7381 gtggttaaag ccctggccga aagtccgtgg ggtgtcttca ccgtttgctc aaccgtaccg
     7441 attgcgctgt ttatgggtat ctacatgcgc tttatccgtc cggggcgtgt gggtgaagtc
     7501 tctgttattg gtatcgtgct gctggttgcc tctatctact tcggtggcgt gattgctcac
     7561 gatccgtact ggggtccggc actgaccttt aaagacacca ccattacctt cgcgctgatt
     7621 ggctatgcgt ttgtttccgc actgctgccg gtgtggctga tcctcgcacc gcgcgactat
     7681 ctggcaacct tcctgaaaat cggcgttatc gtcggcctgg cgctgggtat cgtggtgctg
     7741 aacccggaac tgaaaatgcc tgcgatgacc cagtacattg acggtactgg cccgctgtgg
     7801 aaaggcgctc tattcccgtt cctgttcatc accatcgcct gtggtgcggt atctggcttc
     7861 cacgcgctga tctcttccgg tacgacgccg aaactgctgg ctaacgaaac cgacgcgcgt
     7921 ttcattggct acggcgcaat gctgatggag tccttcgtgg cgattatggc gctggttgca
     7981 gcgtccatca tcgaaccggg tctttacttc gcgatgaaca ccccgcctgc tggccttggc
     8041 atcaccatgc ctaacctgca tgaaatgggt ggcgagaacg cgccgatcat catggcgcag
     8101 ctgaaagacg ttaccgcaca cgcggcagcg accgtcagct cctggggctt cgtgatttcg
     8161 ccagagcaga tcctgcaaac cgcgaaagac attggtgagc cttctgtcct gaaccgtgca
     8221 ggtggtgcgc cgacgctggc ggtaggtatc gctcacgtgt tccacaaagt gctgccgatg
     8281 gctgacatgg gcttctggta tcacttcggt attctgttcg aagccctgtt catcctgacc
     8341 gcgctggatg cgggtacccg ttctggccgc tttatgctgc aagacctgct gggtaacttc
     8401 atcccgttcc tgaaaaaaac cgactctctg gttgccggta tcatcggtac tgcgggctgt
     8461 gtgggtctgt ggggctacct gctgtatcag ggcgtggtcg atccgctggg cggcgttaag
     8521 agcctgtggc cgctgttcgg tatctccaac cagatgctgg cagccgtagc gctggtactg
     8581 ggcaccgttg tgctgattaa gatgaagcgc acccaataca tctgggtaac tgttgttccg
     8641 gctgtatggc tgcttatctg caccacctgg gcgctgggtc tgaaactgtt cagcaccaac
     8701 ccgcagatgg aaggcttctt ctacatggca agccagtaca aagagaagat tgctaacggt
     8761 actgacctga cggcgcagca gattgccaac atgaaccaca tcgttgtgaa caactacacc
     8821 aacgcaggcc tgagtattct gttcctgatt gtggtgtaca gcatcatctt ctacggtttc
     8881 aaaacctggc ttgcggtgcg taacagcgac aaacgtactg acaaagaaac accgtacgtt
     8941 ccaatcccgg aaggcggcgt gaagatctct tcgcaccact aaccgtgttt agccccgctt
     9001 cggcggggct ttgttcttat cagagtgaac tatgtttggt aacttaggac aggcaaaaaa
     9061 atatctcggc caggcggcga agatgttgat tggtattcca gactacgaca actacgtcga
     9121 gcatatgaag accaaccatc ccgacaagcc gtacatgagc tatgaagaat tcttccgcga
     9181 acgccagaac gcgcgctacg gcggcgatgg taaaggcggt atgcgctgtt gttaatggag
     9241 agagcatgaa cccgattgca gttaccctac tcaccggttt tttaggcgcg ggcaaaacta
     9301 ccctgctgcg ccatattctt aacgaacaac acggctacaa gattgccgtg attgaaaacg
     9361 aattcggcga agtctccgtt gatgatcaac tgattggcga ccgcgccacg cagatcaaaa
     9421 cgctgaccaa cggctgcatc tgctgttcgc gctccaacga gctggaggac gcactactcg
     9481 acctgctgga taatctcgac aagggcaata ttcagttcga tcgtctggtg attgaatgca
     9541 ccggcatggc cgatcccggc ccgattattc agaccttttt ctcccatgag attttatgcc
     9601 agcgctacct gctggacggc gtgattgcgc tggtggatgc ggtacatgcc gatgagcaga
     9661 tgaaccagtt caccatcgcc cagtcccagg tgggctacgc cgaccgtatt ctgctgacta
     9721 aaaccgacgt cgcaggcgaa gcagagaaac tgcgtgaacg cctggcgcgc atcaacgccc
     9781 gcgcaccggt ctacaccgtc acccacggcg acatcgacct gggcctgctg ttcaacacca
     9841 acggttttat gctggaagag aacgtcgtca gcaccaaacc gcgtttccac tttatcgcgg
     9901 ataaacaaaa cgatatttcg tcaattgtgg tggaactgga ttacccggta gatatcagcg
     9961 aagtttcccg cgtgatggaa aacctgctgc tggaatcggc ggataaactg ctgcgttaca
    10021 aagggatgct gtggattgac ggcgaaccta accgcctgct gttccagggc gtccagcgcc
    10081 tctacagcgc cgactgggac aggccgtggg gcgatgaaaa accgcatagc acaatggtgt
    10141 ttatcgggat tcagttgccg gaggaagaga ttcgggcggc gtttgcggga ttgaggaagt
    10201 aagttcggga aagatcaact ttttgagagt tccaccgcaa cattattacg agtgga
//
LOCUS       DADMGC010000135         9955 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.135, whole genome shotgun sequence.
ACCESSION   DADMGC010000135 DADMGC010000000
VERSION     DADMGC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.135"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..336)
                     /locus_tag="JNZ73_003423"
     CDS             complement(<1..336)
                     /locus_tag="JNZ73_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAY8733579.1"
                     /translation="MYTESNIKLSCNALWQKCVLATLYRAAATHPKIKLLQFKLIPCP
                     GMNKAIPSLIADFTGELHRLISEHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLM
                     VLLLNNDVWC"
     gene            complement(364..926)
                     /locus_tag="JNZ73_003424"
                     /pseudo
     CDS             complement(364..926)
                     /locus_tag="JNZ73_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1130..1336)
                     /locus_tag="JNZ73_003425"
     CDS             complement(1130..1336)
                     /locus_tag="JNZ73_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAY8733580.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1443..2006)
                     /locus_tag="JNZ73_003426"
     CDS             complement(1443..2006)
                     /locus_tag="JNZ73_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAY8733581.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3381..5216)
                     /locus_tag="JNZ73_003427"
     CDS             complement(3381..5216)
                     /locus_tag="JNZ73_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733582.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5317..5604
                     /locus_tag="JNZ73_003428"
     CDS             5317..5604
                     /locus_tag="JNZ73_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY8733583.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5576..7105
                     /locus_tag="JNZ73_003429"
     CDS             5576..7105
                     /locus_tag="JNZ73_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HAY8733584.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7275..8492)
                     /locus_tag="JNZ73_003430"
     CDS             complement(7275..8492)
                     /locus_tag="JNZ73_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8733585.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8859..9845)
                     /locus_tag="JNZ73_003431"
     CDS             complement(8859..9845)
                     /locus_tag="JNZ73_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733586.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFLWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
BASE COUNT         3138 a         2262 c         1846 g         2709 t
ORIGIN      
        1 acaccagaca tcgttattaa gtagcagcac cataagataa ggagtggcat cgttagccac
       61 agcttcccta ctccagaggt aatataaagg ggtgggctcg actgatttat ctttacgtcg
      121 cttacactgc aaatgttcag aaattagtct atgcagctca ccagtaaaat cagcaatcag
      181 agaaggaatt gccttattca taccagggca aggtattaat ttaaactgta ataatttaat
      241 tttaggatgt gtagcagcag cccgatacag agttgcaagg acacactttt gccagagggc
      301 gttacaggaa agcttaatgt ttgattctgt atacataata aatcacctta tagttacaat
      361 aggtcaaaaa ccgctgtagc caaagttacg ctggcctgat gctttagtgt cgagcttcgt
      421 caaataatcc agacgtacca ataagcgctg gtactgctct gggaaattag gatcgttaat
      481 atccaggatg taacgtccat tagcagggaa atgaactaat ccctggtgat cttccagttc
      541 caaccctaag gcactgtacc aggctccggt aattaaccct ctcagggtgt cagagcgctc
      601 atagtttccc agatggtaat aggcgtcttt gttaaaaaaa ggcagacgtg ataatgacat
      661 ttaaaggact gcgtatattc tttcgcccag ataatacaaa ccgacgttgc gtaggtgcgt
      721 ttgccttgtt gttttttacg gtgttgatct gcattaattt tcgcattcag cgagttacgg
      781 aaacgagata tctctccggg gatcaggtta gggaaacagg taatgatatc cccgttgtca
      841 atcacaggag ggtaatggag atccccccgt attgctgtta ttctggaagt gtttttaata
      901 ttcgtattga tagttctcag agatatatca cactctagat attttaatta agtatcagtt
      961 cgatatcggc atattgtaca ctacctcagg tagatgatga gatcagatac agggatcaac
     1021 tgacggtgag cgcagtcccc acattttatc cagggcttac tgcatattcc caacagccat
     1081 tcgttagcca ggtagacgaa taccctgatt tgccgctggt tttgctttcc tatcgaaggg
     1141 cccatacatc atcacgccca cgaaacaacc gacgaaataa cgcaactttc tcatccgtgg
     1201 ataatacgaa aacgcactgc acagggctct gcggtttacg tcgccattca atcccatgag
     1261 cttcaagtaa tgagaccagc cgggtatttt ctgcccgcag tactgctagt tcatcgctgt
     1321 cagatttata aaccatatgc gttatcactt gtacctgtct cccggcggca ccacgactga
     1381 aattcgtgtg aaggcaaatc cacgcgataa cttacgatag ttcggacata agcccccata
     1441 attcagttat gtcgtggtcg accccggtta cttttttcgg gaagcctgct acggcccaga
     1501 ataataccgt gttctttctg atcctgttta gccagatagc taagggcgta acgtaagtcg
     1561 tctaccgcag atttatcact atagtgaatc acgtgatcga tacgcaccgg gtatttgtct
     1621 ttagcccggc acagatgaaa ataaccctcc ccttccgtga tccgcctcca gatatctccc
     1681 agttgcctgg atatctgata agagttcttg tgacgttgtc catccaggta gccgataaaa
     1741 tgaatatgaa gccctttgtc tggtgtatat tccattaccc agtaatatcc cgccaacatc
     1801 gtctgggttt cgctaagtaa acggtatatt tccatacaca tactgtgctt acaggaatgc
     1861 ccgaaactgg gcgtgtcttt cctgtaagca aaatcaattc tgaaaggtaa cagtttagaa
     1921 aaacgttgaa acataccatc catgtgttca ttcacgtctt tcagaatcat gaaatccatt
     1981 ttgtaattgg gattggcatt atacatttta tagtccttac tttataaaac tcacaggaag
     2041 agagaattac gtaaatactg agagtagaga aacgccgtac cacggcatta ataaacgaag
     2101 taaaccagca gcacagacaa cctgataaaa ctgttttgta ttaccgcatt actgtgatga
     2161 ggtaatactt cagaatacag tgagactctc ttaaactgag aaccagtacg aattacaatg
     2221 gaaaaacaca cctcatagaa ttgagtttca ggaagcacca ctaaattctt tacaggaagt
     2281 aatctgacat tcacaaaact cagtatcggc tagctacagg caacatcgct attaatattg
     2341 tcgaaccatt aagtaaagcc gagagagacc acgggtattt aatcattagc ataaaagaat
     2401 aatggtatac agaacatacc cacacaccat aagaagattg ttccatacag aaaagatcat
     2461 atttacctat acctataaac tgtaacggag tcataacacg ctctaatagt gttccgatac
     2521 gtaaccagtg ttaagtacgt acaggacaac attacacaca taattcacca tacagcttcc
     2581 cccatatgct tcactgcgta gtgtatggag atatatgcat tacacacata cagaatactt
     2641 agcataaaga ggagtgacat taccacgtaa agagataagt tatacaacaa acatacaaac
     2701 aatacggcac agacacacaa taccataact ccaactaata atactaatta accttaatta
     2761 tttatggtat atgaaataaa tgtgattctc ccggaactaa ctaacctaac cagacagatg
     2821 atcactgtat aaaaacttca gttaatactc atttgttttg tcgactcagt atataatgag
     2881 tgaccagtaa tcaggataat cacacttaca taacctgtaa aaaagaacaa gaagaataag
     2941 ccactaacat aatgcaaaat ggcatctgaa tcgatcgcag tttggcattc gaatcgatca
     3001 tgatttggcc caatccgtga tcgaatagag cggaatacgc aacataattc accagacaag
     3061 agcttacacc gaattacaga ttatgtatta atgtcaaaac tggcatattt tacagataca
     3121 atgaacatgc aacccatcaa tctaataggg gcggtatgta catctgccca tgaatgggat
     3181 ggcgctaaga atacaaaaca attagacata gatcacaaaa acacaccatt ctaattgaaa
     3241 aacaaaacaa acaaataaat catactatta tagaattttc tcttgccaga catagataat
     3301 acccctgcta attttgctaa tggttagcaa aattagcagg gtatataaag attattgacc
     3361 caaaacacac tcagatgaat tcacgacagc tcaggagggt tattcctgat gtcatcttca
     3421 caaataatgg ttaacgcatc atatatcatt ctgcgtctgt tcctgttttc ggccttatcg
     3481 ccatacaaca tcatcacatt tttaatttca tcgaccggtg atttcacata gtgaactaat
     3541 acaccaagct tctcaacaca ttgcagtaaa gtatatttct tactgcctgt gaaaagccct
     3601 atcatagttt ctttacgttc gcgactggat aatactttgt tttccataat gacttctgcc
     3661 agcgtcttac catcataaac atactcttta tctatcagaa tatttatctt atccagcacc
     3721 ttctctacgt catcaagcaa acctgtaata tatgggggcg caatatatgg tttcgtactg
     3781 atcttttcaa gcatacaatt atacatctta ataacacgca tcagggtaaa attgttcgca
     3841 tcatactgta tttcaccctc cttatataca ggatcaataa gtggtatttt ttcaataaat
     3901 gcatcctgat aggcttttac atggtcaagc caataaaaca acgagccata ctttatattt
     3961 tttctattta ctttaacctc cagtgctgtc cgaagcaacg ataatgcccc cctgacatat
     4021 cctgaaggta attcaccaaa caatcctttt ctttccatgt catcataatg ctgcagcaca
     4081 tcattaattt tatttttcct tatcagacaa atgtacaccg caagtgtttc gtagcagctg
     4141 gtcacatgaa cagcctcatt ctgcaactgc ccaaaatagc ttataatctt tttcagatat
     4201 gcgtcatcac aaccattctt atcattaata ttaaatacaa gatctttcag acgggaaata
     4261 tattcctgag atttatcatc aagtgaaaca ctataacgag acaatgttcc ttctgcactg
     4321 ttttgaaact tataaaacat ttccagtaat ttttttgcat aaggcgttgt atcaaagctg
     4381 gtaaagtcat aaagatattg cgcagcgtaa ccatacaaac catgaatcca gagtccttct
     4441 ctcaccaaca ctctgagata atatgcaagg ctttgaagta tcttcgtctt tcgttgtata
     4501 tcaagtaaga cacttaatga ataaaatgca ttatagaatt tatcaatatt cctgagcact
     4561 ttctccagtt catccttatc cagcttcttc tcccggtaat cctttttata ttcatttttg
     4621 atagccatga tatattgttg catcaggctc ttgcttttcc cgttatcctt ccgtatagtt
     4681 cctgtatatt tccccagagc atacagcttt ccattttcat gaaaatcttc cttgttgaat
     4741 cccagttcag ccagatattc ccgaagataa cgtttaacag cagcttcatc gctttctgta
     4801 tcagcgtgat caagacaagg acaatacgat ttctgtaaaa aattataaat atgataataa
     4861 aaagcagagt cattctctgg tctgtatgta ttatacatat ctacaacaag aaaaataacc
     4921 ccaaaagtgc gcagacaata gctgcgaagg aattcaggtt ttagtgtgga atgcactgga
     4981 taagaattca tcatgtaata aaaatgttca tatcgggcat atatgatccg ccgttgctcc
     5041 tgactgaaca ccccttcagc atcaataata cgactgatct cattataacg ttgatcccag
     5101 aatttttgat gcatttcaaa attggtactc ttccgcttgt gtgccggatt ctcgccttcc
     5161 ttaaacaacc ccaaaacagt acaaatatca tacaacatat ttgtaaatgt gcgcatatgc
     5221 tgtgctctcc ggttggtttg cattttatcg cagaggacat tctgagctta acgtatcaaa
     5281 ctgacacaac gacatgcaat gcatatggag aacaatatgt caaatcaaac cggacagctt
     5341 tacacgcctt cacgcatcct gcgtatggct gaacttaccc gactgctggg tatatcaaga
     5401 tctactattt acgtgaaaat aaatccggcc tcgaaatacc acgaccctac ctttcccaaa
     5461 ccaataaggc ttggtagtgc atccgtaggc tggcgggcat ctgccatcga tgagtggctg
     5521 atgttacata cggcaccagc ctggtctgaa cctgaaaaca aactaaagga acttaatgat
     5581 gacgacaaaa ctatcactcc ctgaacttga cacaactaaa ctgcctgaca ggtgccaggc
     5641 attacttgat gaaatgcatg aagaaacagg aatacgccgt gaaatacttc tgtctgttat
     5701 gctgacggtc atggctgcct cggtccagga tacgcatgaa gttgaacttt ccggagggca
     5761 acgtaccagc cttcagatat ttatgtgtct ttcatcagca tcaggcagtg gaaaaacctc
     5821 tgcctgcgca aaattaatcg ctcctgttca tgaaacagaa gaagaactac atcaggcata
     5881 tatcgacgat aaaaaaaact atgaccgaat gatggaaatg tggacaacag ataaaaaaat
     5941 cctggagcgg agatataaaa aggaaattga aagatccccg gaaaatgctg ctgctgcacg
     6001 agcagcactt gaaaagtgta tagcaaataa acccgaacct cccgtacaac aggttctcat
     6061 cgtgaatgac gcgacaccag aagggatagc tttgaaactc agccagtcac cctctcttct
     6121 gttgctgtct gatgaaggag gtactattct ggataagcgt ttcgaacgca aatcggcact
     6181 gtacaacaca ttatggagtg ggcaaccagt caacgttgaa cgggcatcca gacccgggtt
     6241 ccggctgaat gatgtccgtc tcactatact tatactgact caaccggtaa tatttaataa
     6301 gttttttact ctcactggcg atcagatccg gggcaatggc tttctggccc ggttgctatt
     6361 ttgcgaaccc ggagcaatta aaataatgac gacagaaccg catactgcca ctcctgttgt
     6421 gacgcaacaa tgtgcaagct gttttcgtaa gaaaagtttt ggcagtcaga tcagagactc
     6481 tctgagagca tccagagaaa gacgtgcaaa aagtaaagag cgcatctgca tgacattatc
     6541 aaatgcagca tcccgcgcac tggagcgctt tcacaaagaa aatatggata ctgtcagaca
     6601 gaatcctcat atgggtacat ttgaagacat tattgtcaga aaaagagagc aggttgtcag
     6661 gatagcagca cttctggaac tggagaaaga tccagactcg acagtaataa cgctggaaag
     6721 tactaatagc gccatttatc tcattgaatt ttatttcaag catcttatat ataaacttga
     6781 atcccttcgg gaaatatccc cggctgagaa acttgataaa tggcttcaga aaagaattat
     6841 cacaacagca ggttacatat tccagaaaag ttatatttta caatatgccc catatgctct
     6901 caggaaaaaa tgtgttctgg atgaagcact tgacatactg gcggaacaaa ggaaaataag
     6961 gattgatgat aacctggttg tttatatagg aaacacgatt acaccatccg aactggcaaa
     7021 aaaactaaat attcctgcat tcgatgccgg agttttcatt tgtgatcatc aaaacatact
     7081 gaagtatcac cgaaacagac tatagatact atcggacaaa aaataaatta tcacaagtgt
     7141 attattcgtt ataataatga ataatcgtta tctgagtgca atgccaggtt ttcattacga
     7201 actggcattg catttatttt tcaaaaaaga aagcagtaac catttggcta ctctgagtat
     7261 gccagtccat gaaactaagc gaattcctta gtgtttttag caagttgaga catcacataa
     7321 tccgcccatg cctgcatcat cggtacacgc tgctccagta gatcagtacg atgatatgcc
     7381 gcctcaacct tatttttcag cgtatgagcg agcgcccttt ccgccagatc ccgcgaatac
     7441 ccctgttcac tacaccagtc cctgaatgtt gagcgaaaac catgtgccgt ggcaactcgc
     7501 cccggaatgt cactgacggc tttcttttta cgcagaaaac ttgtcaacac catatcggaa
     7561 aggatctgct gctttctggg tgaagggaac accagttcat catgcaggcc acgtatattt
     7621 tccagaatgt aaatagcctg ccgggataaa ggaacacgat gctgtagcct agctttcatt
     7681 ctttctgcag gtatagtcca tacccgctta tgaaaatcaa tttcagccca gcgcattccc
     7741 ctggcttcgc ccgagcgagt tgctgtaagt atcaccatta ataacagtgc gcgggtaaca
     7801 ttataaggtt catcactata cacactggtc gccacaaaaa gcggtaactg cctccagggc
     7861 attgcgggtt ggtgttcatc acgtcctctt gtctgctgag gaagcaaatg gtcaaccaca
     7921 tcaacaggat ttgccacaca aaaaccgtgc gcccatcccc actgcataac aacatgaatg
     7981 cgctgtttaa cccggcttgc cgtttctgac aaggttaacc agactggacg cagtgtttct
     8041 gccacgtccg cagccgtaat cgaatccagc gtttttgctc ccagttgagg aaacgcgtaa
     8101 ttctcaagcg tcgataacca ctgccttaca tgctttggat tctcccatcc aggagacagt
     8161 tctgcatgta cacgcctggc tgcatcggca aatgttggga taacgacttt ctcagattca
     8221 gcctttttaa tctccagagg atcatcacct gcagcaagtt gctctcgcat tatccgggca
     8281 gtacgtgcag cttcagcaat actgacctct gggtaagttc ccaatccagc attacgtctt
     8341 ttttgtgtca ccggacttac ataacgaaaa acccatttcc cccgcccctt tactgaagaa
     8401 ggatgaaggg tcagtccggt aattccccca tggggcaatg gtttgtcatc aggtttgata
     8461 tgtcttgctt tcgtatccgt caatactgcc atacgctaat tcccgtcact ctggtatgcc
     8521 atccagtatg ccattagtcc ctcgcttcac taagaataca ttagataaca tcggacaaca
     8581 aaaagtgtaa tacattgttt ataaagaaat tatataacac ccctggatta agtcagattt
     8641 atttcaggcg gtccccctca ccgccatatt taaagaagag cgcgtacgaa agtacgggct
     8701 tttttttcgt atattgtaca caccgggtgc ccccgcccac gtctgttagg gcgaggggaa
     8761 attgtgcaca tcaactgtct ggttactcag tcaacaatca aaatgcgcaa gatagtacag
     8821 aacatatatg cgttatctgg tcacttcctg taacttcctc acttattaaa atgcccccaa
     8881 cggaaataca tgtcacatgc catcataaaa tcgttatgcc agaggaatcg acaatggaaa
     8941 aaatagtaaa ttaagcacgg tacatagcag agtaaaaaat aggcttcaaa ccaaatccgc
     9001 cattccacaa gcgttgccaa taccgacacc agcgtaatcc acatcatttc aataaccagc
     9061 aatatgaata accgaagcca aagcggcttg gcaaaaaagg tcgacaacgt attttcactg
     9121 gagaaaaaca gatcgatttc ctgcaaaaaa aacctgccca gaaaagcgta ccgcttggag
     9181 aagcgcagaa aaaacagatt aatcgccaac actacgccac aaagaatgag caatgcaatt
     9241 atcgatttaa acgttaacca ctgcccctct gtcacaaaag aatcactgtt acgcaacggg
     9301 ctgatagtaa gcaggttaac aaccgttgag atgcacaccg aaagcagcac atagcgagtc
     9361 agacttttat attttacttt tttcgctact gaaatagcga cctgcttgac ataactggtg
     9421 cggatcatcc gaaacagcag aaagatgacc aacggcccaa gtaacgaaat caggaacagc
     9481 gcatataacg agggtagaag ctggtatagc ccccaactca ttgcactcaa catgattaac
     9541 gacagaaatc ctgccaccag aggttgtgta agaaataatt ctattttgtg gttctgcttt
     9601 ttcttaaacc acaaaaaatc gatattcttc gtgacgcatt tataagccca gtacgccttg
     9661 agatcaaaaa taaaattata cagcaccagg gccagaatcc cactaactac accaatatcc
     9721 tctgatggaa aagtaaaccc tgcatttttc caataaaagc ctatcagaat aagtccaata
     9781 gcataaatgt atatcagatt tcgtttatcc tgaaagcgaa taaaagttaa ataatcagga
     9841 atcatttgaa tacctttgca tcactaaatg ctttacgaca atgcataact cgttgctgat
     9901 ccacgccagc aaaatcattt aacatattcc ccaaataggg cttattactt atttc
//
LOCUS       DADMGC010000136         9955 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.136, whole genome shotgun sequence.
ACCESSION   DADMGC010000136 DADMGC010000000
VERSION     DADMGC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.136"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JNZ73_003432"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JNZ73_003432"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAY8733587.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JNZ73_003433"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JNZ73_003433"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HAY8733588.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3326)
                     /locus_tag="JNZ73_003434"
                     /pseudo
     CDS             complement(2698..3326)
                     /locus_tag="JNZ73_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3456..5216
                     /locus_tag="JNZ73_003435"
     CDS             3456..5216
                     /locus_tag="JNZ73_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HAY8733589.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5622..6479
                     /gene="focA"
                     /locus_tag="JNZ73_003436"
     CDS             5622..6479
                     /gene="focA"
                     /locus_tag="JNZ73_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HAY8733590.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6534..8816
                     /gene="pflB"
                     /locus_tag="JNZ73_003437"
     CDS             6534..8816
                     /gene="pflB"
                     /locus_tag="JNZ73_003437"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY8733591.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9008..9748
                     /gene="pflA"
                     /locus_tag="JNZ73_003438"
     CDS             9008..9748
                     /gene="pflA"
                     /locus_tag="JNZ73_003438"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HAY8733592.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2555 a         2495 c         2374 g         2531 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcttggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acatcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagagc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccatta aagaactcaa
     3001 attccgtcat gttggagtta ttgagtttcg accaggccag ctcgccatca cattaccagg
     3061 cggtataaca acgccagagt gataaagacg ataagtaccg gcaccatcgg cacatcatca
     3121 taaaacgcca catctcccgc cgctgaaccc agcgtgagaa tgattaacac ttcaaacagc
     3181 gacatctgtc gcacaccgcg tcttccggtc attttgagga acaaaaaaac cagtacaaag
     3241 gtataaagac tacgtagtgc aacttcgcca aggaaatcaa aaggcacttt atcaaatgcc
     3301 atacggtgga gatcgaacgc cttcattttt atgctcttaa cgtggtttat ccgctaaaag
     3361 catagtcaac agttcatcgg catggcggcg gtgatagcgc ctttgagata aaacccgtat
     3421 tattgcgcgc tttccgtacg actaaagtga ttttcatgac gcaaacattt atccccggca
     3481 aagatgccgc tctggaagat tccatcgctc gcttccagca aaaactttca gacctcggct
     3541 ttcagattga agaggcctcc tggctgaatc ccgtgcctaa cgtctggtct gtacatattc
     3601 gcgacaaaga gtgcgcactg tgttttacca acggtaaagg cgcaaccaag aaagcggcgc
     3661 tggcttctgc actcggtgaa tatttcgagc gtctctcaac caactacttt tttgctgatt
     3721 tctggctggg cgaaaccatc gccaacggtc catttgtgca ttatcccaac gaaaaatggt
     3781 tcccactgag cgaaaatgac gatgtgccag aagggctgct cgatgaccgt ctgcgcgcat
     3841 tttacgatcc ggagaatgaa ttgaccggca gtatgctgat tgacctacaa tccggtaacg
     3901 aagatcgtgg tatttgcggt ctgccgttta cgcgccagtc cgacaatcag accgtttata
     3961 ttccgatgaa tatcattggt aacctgtacg tttctaacgg tatgtccgct ggcaataccc
     4021 gcaacgaagc acgcgttcag gggttgtccg aagttttcga acgctacgtg aaaaaccgca
     4081 ttattgctga aagcatcagc ttgccggaaa tcccggcaga cgtgctggcg cgttacccag
     4141 cggtggttga agcgatcgaa acactggaaa cagaaggttt cccgatcttc gcatatgatg
     4201 gttcccttgg cggccagtat ccggtgattt gcgtggtact gtttaatcct gcaaacggca
     4261 cctgctttgc ctctttcggt gcgcatcctg attttggcgt agcactggaa cgtaccgtga
     4321 ccgagctgct gcaaggtcgt ggcctgaaag atttggatgt gtttactccg ccaaccttcg
     4381 atgatgaaga agtcgctgaa cataccaacc tcgaaacgca ctttatcgat tccagcggtt
     4441 taatctcctg ggacctgttc aagcaggatg ccgattatcc gtttgtggac tggaatttct
     4501 ccggcaccac ggaagaagag tttgctacgc tgatggctat cttcaacaaa gaagataaag
     4561 aagtttatat tgccgattac gagcatctgg gcgtttatgc ttgccgtatt atcgtgcctg
     4621 gcatgtccga tatttatccg gctgaagatc tgtggctcgc gaataacagt atgggcagcc
     4681 atttacgtga aacgattctt tcgctaccag gcagcgagtg ggaaaaagaa gattacctga
     4741 acctcatcga gcaactggat gaagaaggtt ttgatgactt tacccgcgtg cgtgagctgt
     4801 tgggtctggc gaccgggtcg gataacggtt ggtacaccct gcgtatcggt gaattaaaag
     4861 ccatgctggc gctggctggt ggcgatctgg aacaggctct ggtctggacc gaatggacga
     4921 tggagtttaa ctcatcagta tttagtccgg aacgcgccaa ctattatcgc tgcctgcaaa
     4981 cgttgttatt actggcacag gaagaagatc gccagccgct gcaatatctg aatgcgtttg
     5041 ttcgcatgta cggcgcagat gccgtagaag ccgccagtgc ggcaatgagc ggcgaagcgg
     5101 cgttttacgg cctgcaaccg gtagatagcg atctgcacgc gtttgctgca catcagtcgt
     5161 tgttgaaggc ctacgaaaag ctgcaacgcg ccaaagcagc attctgggca aaataaaatc
     5221 aaatagccta cgcaatgtag gcttaatgat tagtctgagt tatattacgg ggcgtttttt
     5281 taatgccccg ctttacatat atttgcatta ataaaataat tgtaattata aggttaaata
     5341 tcggtaattt gtatttaata aatacgatcg atattgttac tttattcgcc tgatgctccc
     5401 ttttaattaa ctgttttagc ggaggatgcg gaaaaaattc aactcatttg ttaattttta
     5461 aaatttattt ttatttggat aatcaaatag ttactccgta tttgcataaa aaccatgcga
     5521 gttacgggcc tataagccag gcgagatatg atctatatca atttctcatc tataatgctt
     5581 tgttagtatc tcgtcgccga cttaataaag agagagttag tgtgaaagct gacaaccctt
     5641 ttgatctttt acttcctgct gcaatggcca aagtggccga agaggcgggt gtctataaag
     5701 caacgaaaca tccgcttaag actttctatc tggcgattac cgccggtgtt ttcatctcaa
     5761 tcgcattcgt cttctatatc acagcaacca ctggcacagg cacaatgccc ttcggcatgg
     5821 caaaactggt tggcggcatt tgcttctctc tggggctgat tctttgtgtt gtctgcggag
     5881 ccgatctctt tacttccaca gtgttgattg ttgttgctaa ggcgagtggg cgcatcacct
     5941 ggggtcagtt ggctaaaaac tggctaaatg tctattttgg caatctggtc ggcgcactgc
     6001 tgtttgtact tttaatgtgg ctttccggcg agtatatgac cgcaaatggt caatggggac
     6061 taaacgtcct acaaaccgcc gaccacaaag tgcaccatac ttttattgag gccgtctgtc
     6121 ttggtatcct ggcaaacctg atggtatgtc tggcagtatg gatgagttat tctggccgca
     6181 gcctgatgga caaagcgttc attatggtgc tgccggtcgc gatgtttgtt gccagcggtt
     6241 ttgagcacag tatcgcaaac atgtttatga tcccgatggg tattgtaatc cgcgacttcg
     6301 catctccgga attctggacc gctgtcggtt ctgcaccgga aaatttttct cacctgaccg
     6361 tgatgaactt catcactgat aacctgatcc cggttacgat cggtaatatt atcggcggtg
     6421 gtttgttggt tgggttgaca tactgggtca tttacctgcg tgaaaacgat caccattaat
     6481 ggttgtcgaa gtacgcagta aataaaaaat ccacttaaga aggtaggtgt tacatgtccg
     6541 agcttaatga aaagttagcc acagcctggg aaggttttac caaaggtgac tggcagaatg
     6601 aagtaaacgt ccgtgacttc attcagaaaa actacactcc gtacgagggt gacgagtcct
     6661 tcctggctgg cgctactgaa gcgaccacca ccctgtggga caaagtaatg gaaggcgtta
     6721 aactggaaaa ccgcactcac gcgccagttg attttgacac cgctgttgct tccaccatca
     6781 cctctcacga cgctggctac atcaataaag cgttggaaaa agttgttggt ctgcagactg
     6841 aagctccgct gaaacgtgct cttatcccgt tcggtggtat caaaatgatc gaaggttcct
     6901 gcaaagcgta caaccgcgaa ctggacccga tgatcaaaaa aatcttcact gaataccgta
     6961 aaactcacaa ccagggcgta ttcgacgttt acactccgga catcctgcgt tgccgtaaat
     7021 ccggtgttct gaccggtctg ccagatgctt atggccgtgg ccgtatcatc ggtgactacc
     7081 gtcgcgttgc gctgtacggt atcgactacc tgatgaaaga caaatacgct cagttcacct
     7141 ctctgcaggc tgatctggaa aacggcgtaa acctggaaca gactatccgt ctgcgcgaag
     7201 aaatcgctga acagcaccgc gctctgggtc agatgaaaga aatggctgcg aaatacggct
     7261 acgacatctc tggtccggca accaacgctc aggaagctat ccagtggact tacttcggct
     7321 acctggctgc tgttaagtct cagaacggtg ctgcaatgtc cttcggtcgt acctccacct
     7381 tcctggatgt gtacatcgaa cgtgacctga aagctggcaa gatcaccgaa caagaagcgc
     7441 aggaaatggt tgaccacctg gtcatgaaac tgcgtatggt tcgcttcctg cgtactccgg
     7501 aatacgatga actgttctct ggcgacccaa tctgggcaac cgaatctatc ggtggtatgg
     7561 gcctcgatgg tcgtaccctg gttaccaaaa acagcttccg tttcctgaac accctgtaca
     7621 ctatgggtcc gtctccggaa ccgaacatga ccattctgtg gtctgaaaaa ctgccgctga
     7681 acttcaagaa attcgccgct aaagtgtcca tcgacacctc ttctctgcag tatgagaacg
     7741 atgacctgat gcgtccggac ttcaacaacg atgactacgc tatcgcttgc tgcgtaagcc
     7801 cgatgatcgt tggtaaacaa atgcagttct tcggtgcgcg tgcaaacctg gcgaaaacca
     7861 tgctgtacgc aatcaacggc ggcgttgacg aaaaactgaa aatgcaggtt ggtccgaagt
     7921 ctgaaccgat caaaggcgat ctcctgagct acgatgaagt gatggagcgc atggatcact
     7981 tcatggactg gctggctaaa cagtacatca ctgcactgaa catcatccac tacatgcacg
     8041 acaagtacag ctacgaagcc tctctgatgg cgctgcacga ccgtgacgtt atccgcacca
     8101 tggcgtgtgg tatcgctggt ctgtccgttg ctgctgactc cctgtctgca atcaaatatg
     8161 cgaaagttaa accgattcgt gacgaagacg gtctggctat cgacttcgaa atcgaaggcg
     8221 aatacccgca gtttggtaac aatgatccgc gtgtagatga cctggctgtt gacctggtag
     8281 aacgtttcat gaagaaaatt cagaaactgc acacctaccg tgacgctatc ccgactcagt
     8341 ctgttctgac catcacttct aacgttgtgt atggtaagaa aactggtaac accccagacg
     8401 gtcgtcgtgc tggcgcgccg ttcggaccgg gtgctaaccc gatgcacggt cgtgaccaga
     8461 aaggtgctgt agcgtctctg acttccgttg ctaaactgcc gtttgcttac gctaaagatg
     8521 gtatctccta caccttctct atcgttccga acgcactggg taaagacgac gaagttcgta
     8581 agaccaacct ggctggtctg atggatggtt acttccacca cgaagcatcc atcgaaggtg
     8641 gtcagcacct gaacgttaac gtgatgaacc gtgaaatgct gctcgacgcg atggaaaacc
     8701 cggaaaaata tccgcagctg accatccgtg tatctggcta cgcagtacgt ttcaactcgc
     8761 tgactaaaga acagcagcag gacgttatta ctcgtacctt cactcaatct atgtaattag
     8821 atttgactga aatcgtacag taaaaagcgt acaataaagg ctccacgaaa gtggggcctt
     8881 ttttagcacg agagcctttt ttgtcagcta tctatacttt aaggtgactg ccaaaacaga
     8941 ctcgacgtag ccttcgagct gcgcaccaac acggcctcag atgggccaca tctggagaaa
     9001 caccgcaatg tcagttattg gtcgcattca ctcctttgaa tcctgtggaa ccgtagacgg
     9061 cccaggtatt cgctttatca cctttttcca gggctgcctg atgcgctgcc tgtattgtca
     9121 taaccgcgac acctgggaca cgcatggcgg taaagaagtt accgttgaag atttgatgaa
     9181 ggaagtggtg acctatcgcc actttatgaa cgcttccggc ggcggcgtta ccgcatccgg
     9241 cggtgaagca atcctgcaag ctgagtttgt tcgtgactgg ttccgcgcct gtaaaaaaga
     9301 aggcattcat acctgtctgg ataccaacgg ttttgttcgt cgttacgatc cggtgattga
     9361 tgaactgctg gaagtaaccg acctggtaat gctcgatctc aaacagatga acgacgagat
     9421 ccaccaaaat ctggttggag tttccaacca ccgcacgctg gagttcgcta aatatctggc
     9481 gaacaaaaat gtgaaggtgt ggatccgcta cgttgttgtc ccaggctggt ctgacgatga
     9541 cgattcggcg catcgcctcg gtgaatttac ccgtgatatg ggtaacgttg agaaaatcga
     9601 acttctcccc taccacgaac tgggcaaaca caaatgggtg gcgatgggtg aagaatacaa
     9661 actcgatggt gttaaaccac cgaagaaaga gaccatggaa cgcgtgaaag gcattcttga
     9721 gcagtacggt cataaggtaa tgttctaatt tccccgtaaa gcggcaactt tattgagttg
     9781 ccgctttttt attccgcgct tcgttctgct acagcgtttt taacacatct tatagtgcat
     9841 ctaccatatc ccgtacctga gataataacg actgataata gctcgcgtga agttcttccc
     9901 catcgaatac cactgtatta cacaagaaag tgacatcggc atcttcaata ccaag
//
LOCUS       DADMGC010000137         9772 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.137, whole genome shotgun sequence.
ACCESSION   DADMGC010000137 DADMGC010000000
VERSION     DADMGC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.137"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            49..579
                     /locus_tag="JNZ73_003439"
     CDS             49..579
                     /locus_tag="JNZ73_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY8733593.1"
                     /translation="MSITGVNIGRLFREGFSNYDPIGVLNAMASQRTKEARGGELQIN
                     ELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGAAKMLFSHAQKTLAWDAFAFT
                     EVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAV
                     QEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="JNZ73_003440"
                     /pseudo
     CDS             597..1235
                     /locus_tag="JNZ73_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="JNZ73_003441"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="JNZ73_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HAY8733594.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="JNZ73_003442"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="JNZ73_003442"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HAY8733595.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="JNZ73_003443"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="JNZ73_003443"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HAY8733596.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGLLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="JNZ73_003444"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="JNZ73_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HAY8733597.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="JNZ73_003445"
     CDS             complement(5552..6613)
                     /locus_tag="JNZ73_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HAY8733598.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="JNZ73_003446"
     CDS             complement(6610..8007)
                     /locus_tag="JNZ73_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HAY8733599.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQTTTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="JNZ73_003447"
     CDS             8163..9161
                     /locus_tag="JNZ73_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY8733600.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKKSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..>9772)
                     /gene="ompG"
                     /locus_tag="JNZ73_003448"
     CDS             complement(9272..>9772)
                     /gene="ompG"
                     /locus_tag="JNZ73_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HAY8733601.1"
                     /translation="RPDWDVKLTDDLRFNGWLSMYKFANDLNTTGYADTRVETETGLQ
                     YTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYLPLTLGNHSVTPYTRIGLDR
                     WSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYAFEWQDHDEGDSDKFHYAGV
                     GVNYSF"
BASE COUNT         2474 a         2598 c         2344 g         2356 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgaacaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcggcaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctagctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc ccacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca gcgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcggcttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccta ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgctgcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagcgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcaaactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcataggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtgccg
     5701 tcagaagtcc agctccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaggcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgtctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcgtc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgcc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagccggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gaatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag aaaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actccagcga tccagcccaa
     9481 tgcgcgtata cggcgtaacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggg cg
//
LOCUS       DADMGC010000138         9750 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.138, whole genome shotgun sequence.
ACCESSION   DADMGC010000138 DADMGC010000000
VERSION     DADMGC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9750
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.138"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            455..1573
                     /locus_tag="JNZ73_003449"
                     /pseudo
     CDS             455..1573
                     /locus_tag="JNZ73_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
     gene            1639..1887
                     /locus_tag="JNZ73_003450"
     CDS             1639..1887
                     /locus_tag="JNZ73_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HAY8733602.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLSAIEYFVLRFIWRRWFSLAD"
     gene            1952..2320
                     /locus_tag="JNZ73_003451"
     CDS             1952..2320
                     /locus_tag="JNZ73_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY8733603.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPRYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2414..3067
                     /gene="nfsB"
                     /locus_tag="JNZ73_003452"
     CDS             2414..3067
                     /gene="nfsB"
                     /locus_tag="JNZ73_003452"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HAY8733604.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3175..4422
                     /locus_tag="JNZ73_003453"
     CDS             3175..4422
                     /locus_tag="JNZ73_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAY8733605.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4490..5866)
                     /gene="pheP"
                     /locus_tag="JNZ73_003454"
     CDS             complement(4490..5866)
                     /gene="pheP"
                     /locus_tag="JNZ73_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HAY8733606.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMTLVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5967..9102)
                     /gene="cusA"
                     /locus_tag="JNZ73_003455"
                     /pseudo
     CDS             complement(5967..9102)
                     /gene="cusA"
                     /locus_tag="JNZ73_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(9114..9655)
                     /locus_tag="JNZ73_003456"
                     /pseudo
     CDS             complement(9114..9655)
                     /locus_tag="JNZ73_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2397 a         2697 c         2355 g         2301 t
ORIGIN      
        1 aaagggtatt ttggctttga catattaggg gctattccat ttcatcgtcc aacaaaatgg
       61 gtgcagtaca gggttgatag aaatatctcc tggggcttca tctttccaga cctcagagta
      121 aacctgaaat cggaaagctt caggcacccg cagttatctt acccttcctc aacaaaatag
      181 ctgtttattt atacagcaaa tcctgtttcg gacttctcac tccccatttg atcaactgaa
      241 gctgaacccg tttttttctg catgcctcaa tcgtcaaaat tcttcgccac cagccagctc
      301 tgccataact tgccacattg aaaaagtagc caaagtgctt caggcaccat cagttcacca
      361 tcaccaaaaa aaagaaacat tccagaactt ccgcatctcg cccagccgct ccgacttcta
      421 tactgaatag aaaacgtcaa cttaagagaa acctatgcca ttacccgatt ttcatttttc
      481 tgaacctttt accctcggta ttgaactgga aatgcaggtg gttaatccgc cgggctatga
      541 cttaagccag gactcttcaa tgctgattga cgcggttaaa aataagatca cggccggaga
      601 ggtaaagcac gatatcaccg aaagtatgct ggagctggcg acggatgttt gccgtgatat
      661 caaccaggct gccgggcagt tttcagcgat gcagaaagtc gtattgcagg cagccgcaga
      721 ccatcatctg gaaatttgcg gcggtggcac gcccccgttt cagaaatggc agcgtcagga
      781 ggtatgcgac aacgaacgct atcaacgaac gctggaaaac tttggctatc tcatccagca
      841 ggcgaccgtt tttggtcagc atgtccatgt tggctgtgcc agtggcgatg acgccattta
      901 tttgctgcac ggcttgtcac ggtttgtgcc gcattttatt gccctttccg ccgcgtcgcc
      961 atatatgcag ggaacggata cgcgttttgc cccctcacga ccgaatattt tttccgcctt
     1021 tcctgataat ggcccgatgc cgtgggtcag taactagcaa caatttgaag ccctgtttcg
     1081 ctgtctgagt tacaccacga tgatcgacag cattaaagat ctgcactggg atattcgccc
     1141 cagtcctcat tttggcacgg tggaggttcg ggtgatggat accccgttaa cccttagcca
     1201 cgcggtgaat atggcgggat taattcaggc caccgcccac tggttactga cggaacgccc
     1261 gttcaaacat caggagaaag attacctgct gtataaattc aaccgtttcc aggcctgtcg
     1321 ctatggtctg gaaggcgtca ttaccgatcc gcacactggc gatcgtcgac cgctaacgga
     1381 agataccttg cgattgctgg aaaaaatcgc cccttcagca cataaaattg gcgcatcgag
     1441 cgcaattgaa gcactgcatc gccaggtcgt cagcggtctg aatgaagcgc agctgatgcg
     1501 cgatttcgtc gccgatggcg gctcgctgat tgggctggtg aaaaagcatt gtgagatctg
     1561 ggccggtgac taagccggga attgccttac agaagaatat ccctgacaat ggtgttttaa
     1621 cttatattta acaatgttat gaaacaccct ttagaaacct tgaccaccgc agcaggcatt
     1681 ttgctgatgg ctttcctctc ttgcctgctg ctgcccgccc ccgcactggg gctgacgctg
     1741 gcacaaaaac tggtgaccat gttccatctg atggatctta gccagcttta cactttattg
     1801 ttttgtctgt ggtttttagt gctgagcgct attgagtatt ttgttctgcg ctttatctgg
     1861 cgacgctggt tctcgctggc ggattaaacg tcgattatcg ccgaacttcg cggcatactt
     1921 tgcttattct ccttcgccta aaggaacgtt tatggataag caatcactgc acgaaacggc
     1981 gaaacgccta gctcttgagt taccctttgt cgagctttgc tggccttttg gcccggagtt
     2041 cgatgttttt aaaattggcg gcaagatttt tatgctgtcg tcggagctac gcggcgtccc
     2101 ctttatcaat ctgaagtccg atccacaaaa atccctgtta aatcagcaaa tatacccaag
     2161 cattaagcca aggtatcaca tgaataaaaa gcactggatt tcggtgtatt ccggcgagga
     2221 aatctccgaa gcgttacttc gcgatctgat caacgattcg tggaatctgg tggttgatgg
     2281 tctggctaaa cgcgatcaaa aaagagtgcg tccaggctaa agcgggaatc tatagcgcat
     2341 ttttctcgct taccatttct cgttgaacct tgtaatctgc tggcacgcaa aattacttac
     2401 acatggagtc tttatggata tcatttctgt cgccttaaag cgtcattcca ctaaggcatt
     2461 tgatgccagc aaaaaactta ccccggaaca ggccgagcag atcaaaacgc tactgcaata
     2521 cagcccatcc agcaccaact cccagccgtg gcattttatt gttgccagca cggaagaagg
     2581 taaagcgcgt gttgccaaat ccgctgccgg taattatgtg ttcaacaaac gtaaaatact
     2641 tgatgcctcg cacgtcgtgg tgttctgtgc aaaaaccgcg atggacgatg cctggctgaa
     2701 gctggttgtt gaccaggaag atgccgatgg ccgctttgcc acgccggaag cgaaagccgc
     2761 gaacgataaa ggtcgcaagt tcttcgccga tatgcaccgt aaagatctgc atgatgatgc
     2821 agagtggatg gcaaaacagg tttatctcaa cgtcggtaac ttcctgctcg gcgtggcggc
     2881 gctgggtctg gacgcggtgc ccatcgaagg ttttgacgcc gcaatcctcg atgcagaatt
     2941 tggtctgaaa gagaaaggct acaccagtct ggtggttgtt ccggtaggtc atcacagcgt
     3001 tgaagatttt aacgctacgc tgccgaaatc tcgtctgccg caaaacacca ccttaaccga
     3061 agtgtaattc tctcttgccg ggcatctgcc cggctatttc ccctcagatt ctcctgattt
     3121 gcataaccct gtttcagccg tcatcatagg ctgctgttgt ataaaggaga cgttatgcag
     3181 gatttaatat cccaggttga agatttagcg ggtattgaga tcgatcacac cacctcgatg
     3241 gtgatgattt tcggtattat ttttctgacc gccgtcgtgg tgcatattat tttgcattgg
     3301 gtggtactgc ggaccttcga aaaacgtgcc atcgccagtt cacggctttg gttgcaaatc
     3361 attacccaga ataaactctt ccaccgttta gcttttaccc tgcagggaat tatcgtcaat
     3421 attcaggcgg tattctggct gcaaaaaggc accgaagcgg cagatattct gactacctgc
     3481 gcgcagttgt ggatcatgat gtatgcgctg ctttcagtct tctcgttgct ggatgttatt
     3541 ttgaatctgg cgcagaaatt cccggcggca tctcagttac cgctgaaagg gatatttcag
     3601 gggattaaac tgatcggcgc aatcctggtc ggcattttga tgatctcgct gctgattggt
     3661 cagtcgccag cgattctgat cagcggtctt ggtgcaatgg ctgccgtgct gatgttggta
     3721 tttaaagatc cgattcttgg tctggtggca ggtattcagc tttccgcgaa cgatatgctg
     3781 aaactgggcg actggctgga aatgccgaaa tacggcgcgg atggcgcggt gatcgatatt
     3841 gggttaacca ccgtcaaagt gcgtaactgg gacaatacca ttaccactat tcccacctgg
     3901 tctctggttt ctgactcctt taaaaactgg agcgggatgt cagcatctgg cgggcgacgt
     3961 attaagcgca gtatcagtat tgatgtcacc agtattcgtt ttcttgatga agacgaaatg
     4021 caacgtctga ataaagcgca tttgttaaag ccttatttaa ccagccgcca tcaggaaatt
     4081 aatgagtgga atcgccagca aggttctacg gagtcggtat taaacctgcg ccgaatgacc
     4141 aatattggaa cctttcgtgc ctatctgaac gaatatctgc gtaaccatcc gcggattcgt
     4201 aaagatatga ccttaatggt acgccaactg gctccaggtg ataacggttt accgctcgag
     4261 atctacgcgt ttactaacac cgtggtgtgg ctggaatatg aaagcattca ggctgatata
     4321 ttcgatcaca tatttgcgat tgtcgaagag tttggtctgc gacttcatca gtcgccaacc
     4381 ggcaatgata ttcgctctct ggcgggtgca tttaagcagt aattaaaaaa accgctctca
     4441 tcgaatggat gagagcggac atcgttttgt cggatgtagc gtgaatgcct tatttccgac
     4501 gcagcgtttt aaatgccaca aacaggaata caatccacac cggcagcagg atcgctgaca
     4561 agcgcatatc atccatcgtg cacatcagca gcaaaatcat gccgaggaag gcgatgcaaa
     4621 gatagttgcc gaacggatag agcagcgcct taaactgtgt ttcacgcccc tgacgtcgca
     4681 tcgccgcgcg aaaacgcaga tgcgccaggc agatcataat ccagttcaac agcaacgttg
     4741 ctaccaccag cgtcatcagc agaccaaacg ctttttgcgg cagcagatag ttgattaaca
     4801 ccaccagcga agtgatcgct ccggaaagca tcagcgagtt aatcggcaca ccgcgacggc
     4861 tgacgcgagt caaaaacttc ggcgcattac cctgcacaga aaggccaaac agcatgcggc
     4921 tgttagagta aaccccgctg ttatacaccg acagcgatgc caccagaatg acgaagttca
     4981 gcgcagaagc taccacattg ctgtcgagat tatggaaaat catcacaaac gggctactgt
     5041 tggatttcac ttccacccac ggatagagtg ccagtaaaac caccagtgaa ccgatataaa
     5101 acagcaggat gcgatacacc acctgattta ccgcttttgg aatgcttttt tccggatcgc
     5161 gcgcttcagc ggctgtaatc ccaatcagct ccagaccgcc gaaggagaac ataattaccg
     5221 ccagcgacaa aatcagccca ttccagccgg tggcgaagaa accaccgtag cgccagaggt
     5281 tgtcgatact ggctttctcg ccgccgtgac cagaaaacag cagccacagg ccaaagccga
     5341 tcataccgat gatcgccagc actttaatca gcgcaaacca gaactcggtt tcgccatata
     5401 agcgcacgtt caccaggtta acggcgttga tgataataaa gaaggcggca gcccaaatcc
     5461 acgttggaac atccgggaac cagtactgca tatagatgcc cgcagcggtc agctctgcca
     5521 ttcccaccag cacgaacatt acccagtagt tccagccaga gaggaagccc gcaaacggtc
     5581 cccagtattt ataggcaaag tgggcaaatg aaccggatac cggctcttcg accaccatct
     5641 cgccaagctg gcgcataatc aggaaagcga tgatcccggc gacgccgtag cccagcaata
     5701 cagccggacc cgccatctga atcgccgggc caatgccaag aaacagacca gtaccaattg
     5761 cgccacccag cgcaatcagt tgaatatgac ggttatgtaa tccgcgatga agcgtcggct
     5821 cttgattcga cgcagtatct tccgatacgg ttgacgcgtt tttcacgcct ttcccctgtg
     5881 tgtctttttt gttgaggggc accttttaac atttagtgcc catcgtcgca agacacaatc
     5941 cacacggtta aacgaggtat cctgctttaa ttccgtaccc gatgtcggtg cagccacatc
     6001 agcttatacg ccgccgggat aataaacagc gacagcaaag gtgcggtgat catgccgcca
     6061 atcatcgggg cggcaatccg gctcatcacc tctgaaccag cccccgttcc ccacagaatc
     6121 ggcagcagac cggcgataat caccgccacc gtcatcgctt tcgggcgcac gcgcaggacc
     6181 gcgccgtgat acaacgcctc atccagcttc tgctcgctga atgtttgtgg attattcaac
     6241 gacggctcgg cctctatggc gtgacgtaaa tacatcagca tcaccacgcc aaattcggcg
     6301 gcgaccccgg caagggcaat aaagccggta cccgtcgcca cagaaagatg aaagcccatc
     6361 caccacagta accagatgcc gcccaccagc gcaaacggta cgctgctgat aatcagcaac
     6421 gcttcgccca cccgacggaa tgccagatac aacagcacga agatgatcat cagtgtcatc
     6481 ggcaccatga gtttcagctt atggttggcg cgctccagta gctcgaactg cccggagaat
     6541 gccacgctgg tgcccggttt taactgcact ttttcagcta tcgctttttg caaatcgtga
     6601 accaccgaca ccatgtcacg atcgcgggca tcgatataaa tccagctcgt cgggcgcgca
     6661 ttctcggttt tcagcatcga cggtccggta gagactttaa cgtcggccac gtctgccagg
     6721 gtgatttgct gcttcatcgg cgtcaggatc ggcaactggc gcagtgcctg cggactatcg
     6781 cgccagcttt gcggataacg cagattaatt ggataacggg caatcccttc caccctttcg
     6841 ccaaccatcg ccccgcccac cgcagaagtc acaaacaact gcacatccgc caccgtcata
     6901 ccgtaacgtg cggctttttc acggttaatc tcaacgttga tatagcgccc accttccagc
     6961 cgctcggcaa gcgcagaagc tacgcctggc accgttcgcg ccacttcttc aatttgctca
     7021 gccatcgcgt cgatatccgc cagcacagtg ccggaaactt taatgccgat ggggctttta
     7081 atgccggttg agagcatatc gatacggtta cgaattggcg gcacccacag attcgccagc
     7141 cccggcagac gcacggtgtt atccagttcc tcaatgattt tgtccattgt catgcctggc
     7201 cgccactgat cctgtggctt aagctggatg gtcgtttcta ccatctccag cggagcagaa
     7261 tcggtggcgg tttccgcttt cccggttttg ccaaataccc gcgccacttc aggtacgctc
     7321 ataattagct tgtcggtttt ttgcagcata ctcgccgcct ctgctgcgga aatccccggc
     7381 agcgtcgatg gcatatacaa caagtcgcct tcattgatct gcggtaaaaa ttccccgcca
     7441 actttattga gcggccagag aaccgtcagc accgaaagcg ccgccaccag cagcgtggtt
     7501 ttcggccagt gcagtacttt cagcaacagc ggatgataaa cacgaatcaa aaagcgattg
     7561 agcggattac tgctttccgg cggaattttg ccacggatcc agtaacccat caggataggg
     7621 atcactacga tcgccagcag cgccgcaccc gccatcgcat acgttttggt gaacgccaac
     7681 gggccaaaca gacgcccttc ctgcccttcc agggtgaaga tcgggataaa cgacaacgtg
     7741 ataatcagca gactgataaa cagcgccggc cccacttcaa cagacgcatc ggtgatcacc
     7801 tgccagcgcg ttttattatc cagcgtggcg tcaggatgct ggtgctgcca ctcttccagc
     7861 cgtttatgcg cattctcgat catgacgata gcggcatcga ccatcgcccc gacggcaatc
     7921 gcaatgccac ccagcgacat aatattggca ttcagcccct ggaagggcat gacaataaaa
     7981 gcaatacaca accccagcgg caacgaaata atcgccacca gcgccgagcg cacatgccag
     8041 agaaacagcg cgcagactac cgccaccaca ataaactctt ccagcaactt gccgctgagg
     8101 ttgtcgatgg cccggtcaat gagctggctg cggtcgtatg ttgtaactat ctccacgcct
     8161 tccggcagac tacttttcag cgtttccagt ttgtccttca cggcggcgat cacttctcgg
     8221 gcgtttttgc cggatcgcag gatcaccacc ccgcccgcca cttcgccttc gccgtttaat
     8281 gccccggcgc atctccgggc caatctggac cttcgcaaca tcgcgcagat aaacgggcac
     8341 gccattttca ctggctttta aaacgatgtg attaaagtcg tcgagcgttt gtagatagcc
     8401 gctggcgcgc accatatatt ctgcttccgc cagttcgatt gacgaaccgc ccgcttcctg
     8461 gtttgaagca tccagcgcgc tttttacttc ggcgagactg atgccatact gcgccaggcg
     8521 ctgaggatcg ataaccacct gatactcttt caccacaccg cctaccgacg ccacttccgc
     8581 aacgtcagga atggttttca gctcatattt gagaaaccag tcctgcaatg agcgtaaatc
     8641 ggcaagatcg tgcttaccgc tgcgatccac cagtgcatat tcatagatcc agccaacacc
     8701 cgtggcatct ggtcccagct cggcgctgac tcccgcaggc agcttaccct gtacctggtt
     8761 gaggtactcc agcacccgcg aacgcgccca gtacggatcg gtgccatctt cgaaaatgac
     8821 atacacataa gagtcgccaa actgcgagaa accacgcaca gtcttcgcgc caggcaccga
     8881 caacatggtg gtggttagcg gataagtcac ctgattttca acgatttgcg gtgcctggcc
     8941 gggatagctg gttttaataa tcacctgcac atcggagaga tccggcaacg catccactgg
     9001 cgtattaatg atggtccagg tgccccagat gctcagaaac aacgcgccca tcagcaccaa
     9061 aaaacggttc gccaccgagc gacgaataat ccattcaatc attggttatt ccctcaatgc
     9121 gcatgggtag cactttcaga gcgcatccgc tccagtgcgc cagaaatatt ggcttcggaa
     9181 tcaatcagga acaggccgcc ggaaaccacc ttttcacctt ccgccagacc agagcgtaat
     9241 gcggtgacgc cttgcgatgc ctggaaaaca gcaacgcgtt tcggtacaaa gcgcccgtcg
     9301 gcatcaacgg taatcacccg ctgttcgttg ccggtatcaa tcagcgcctc gtcggcgttg
     9361 tcgacttcca gacgcagctg cagcgtgcgg gtcgcggcat ccacgccagg tagcagcgtc
     9421 catttgcgga tggtgagtgt tttatccggt cgcgccggaa cggtgagggt aaactgcgag
     9481 gcatctttca ccagccaggc gatggactcc gggatcgcag cagtgaccca caccgggtcc
     9541 ataccctgaa ttcgcgatta cgcgcaatac ccctgagaac cgccatgaac aagggtttca
     9601 tgatttgtga ttttagttta gtacgcaatt tggtacacaa cactattttc acttcagcgg
     9661 gtagtcatca aactcaccat aacgagcatc gttgatgatg tatgtgatca ccacaaatat
     9721 cacatctgga ctagttccgt cttcatgcgc
//
LOCUS       DADMGC010000139         9708 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.139, whole genome shotgun sequence.
ACCESSION   DADMGC010000139 DADMGC010000000
VERSION     DADMGC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.139"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(329..673)
                     /locus_tag="JNZ73_003457"
     CDS             complement(329..673)
                     /locus_tag="JNZ73_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY8733607.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            805..2715
                     /locus_tag="JNZ73_003458"
     CDS             805..2715
                     /locus_tag="JNZ73_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAY8733608.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQLGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSLDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2773..3468
                     /gene="tsaB"
                     /locus_tag="JNZ73_003459"
     CDS             2773..3468
                     /gene="tsaB"
                     /locus_tag="JNZ73_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAY8733609.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3508..4089
                     /gene="yeaY"
                     /locus_tag="JNZ73_003460"
     CDS             3508..4089
                     /gene="yeaY"
                     /locus_tag="JNZ73_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HAY8733610.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRRQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4294..5979
                     /gene="fadD"
                     /locus_tag="JNZ73_003461"
     CDS             4294..5979
                     /gene="fadD"
                     /locus_tag="JNZ73_003461"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HAY8733611.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            6061..7176
                     /gene="rnd"
                     /locus_tag="JNZ73_003462"
     CDS             6061..7176
                     /gene="rnd"
                     /locus_tag="JNZ73_003462"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HAY8733612.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRADWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNARQLRTRQLACLQLLAD
                     WRLRKARECDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMFNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHRKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="JNZ73_003463"
     CDS             complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="JNZ73_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HAY8733613.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8251..8853)
                     /locus_tag="JNZ73_003464"
     CDS             complement(8251..8853)
                     /locus_tag="JNZ73_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-hydroxylating oxygenase subunit alpha"
                     /protein_id="HAY8733614.1"
                     /translation="MLEACPEVHNLKLAARFTTRTPANWKNIVDNYLECYHCGPAHLG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTNEQKSLIEWYRDVFHPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            complement(9210..>9708)
                     /locus_tag="JNZ73_003465"
     CDS             complement(9210..>9708)
                     /locus_tag="JNZ73_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8733615.1"
                     /translation="FAAKCGLSDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQS
                     QSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELLEDKELHSAIELFRKKGFEALMPE
                     IFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEPQD
                     VKKAV"
BASE COUNT         2370 a         2368 c         2559 g         2411 t
ORIGIN      
        1 agttaacctg ttgacattca ggtgttttaa aggtagcagc tcatggtgtg ttatccagta
       61 agatggagaa aattaacggt cagcccgcac gctataacca gattgttcag ctaagaaaga
      121 aaaccagagt tgctaatcaa agggcgaaca caacggcatg gttatgttta tttatatagt
      181 tagttttatg gataaaattg aaattataaa tataaataat gaattagcag aagcatcaac
      241 agtgatatct tattctctgg tcgatttatg taaaaccgac cagtattatc gataaattat
      301 cgtctgactt attttgcttc gataaagctt acaccgcagc cacaatctta atttcaactt
      361 tatacttcgg gttcatcaaa cccgcttgta ccgtgcagcg caccggcgca tgacccgcga
      421 caacccaggc atcccacgct ttattcatcg ccgcgaagtc gtttttatcg gccaggaaaa
      481 tggtggcatc cagaatgctc gatttattgc tgccctgttt ttccagcacg gcgtcaatct
      541 gtgccagcgt gttggcggtt tgctcaaagg catcggcgtc gaggttttcc ggtacaccag
      601 tgtagtagag cgtgttgttg tggattacta catcagacca gcgggcttca gcatcgatac
      661 gaacgatagt catcattttg tcctcattat acttccattt tcgtgctgca aagactgcca
      721 caatgtgagg ttgccgtcac cacttcaact ggcgaaagcg ccctcatcct gacataatcc
      781 ctgttcaaat caacaggggg tagtgtgacg gacgattttg caccagacgg tcagctggcg
      841 aaagcgatac caggctttaa gccgcgagaa ccacagcgac agatggcggt agccgtcacc
      901 caggcgatag aaaaaggcca gccgctggtg gtggaagcag gaaccggtac gggcaaaacc
      961 tacgcttacc tggctcctgc gctgcgggcg aaaaagaaag tcattatctc gaccggctca
     1021 aaagcgttgc aggatcagct ctacagccgc gatttgccaa cagtctcaaa ggcattgaaa
     1081 tatacgggca acgtggcgct gctgaaaggg cgctcaaact acctctgcct cgaacgtctc
     1141 gaacagcagg cgctggcggg gggcgatctg ccggtacaaa tcttaagcga tgtgatcctg
     1201 ctgcgctcct ggtctaatca aacagtcgat ggtgatatca gcacctgcgt cagcgtggcg
     1261 gaagattcac aggcgtggcc gctggtcacc agcaccaacg acaactgtct tggcagcgac
     1321 tgcccgatgt ataaagattg ctttgtggtc aaagcacgta aaaaagcgat ggacgccgat
     1381 gtggtggtgg taaaccatca tctctttctg gcggatatgg tggttaaaga gagtggattt
     1441 ggcgagttga tccctgaagc ggacgtcatg atcttcgacg aagcccacca gctaccggac
     1501 attgccagcc agtattttgg tcagtcactc tccagtcgac aactgctcga cctggcaaaa
     1561 gacatcacca tcgcctaccg caccgaatta aaagacaccc agcagttaca aaagtgcgct
     1621 gatcgtcttg cccagagtgc gcaggatttt cgtctgcaac tcggtgagcc aggttatcgc
     1681 ggtaacctgc gtgagctgtt agctaatccg caaattcagc gggcattttt actgctcgat
     1741 gacaccttgg aactttgtta tgacgtggcg aaactgtcac tggggcgttc cgccttgctg
     1801 gatgcggcat ttgagcgcgc cacgttgtat cgcacacggc tgaagcggct aaaagagatc
     1861 aatcagccgg gctacagcta ctggtacgaa tgcacttcgc gccattttac tctggctctc
     1921 acgccgctca gcgtggcgga taaattcaaa gagttaatgg cgcaaaagcc cggtagctgg
     1981 atcttcacct cagcaacgct gtcggtgaac gacgatctgc atcatttcac ctcgcggctt
     2041 gggatagaac aggcggagtc gttgctattg ccaagcccgt ttgattacag ccgccaggcg
     2101 ttactctgtg tgccgcgcaa tctgccgcaa accaaccagc taggttctgc tcgccagtta
     2161 gcggcaatgc tgcgaccgat catcgaagct aacaacggtc gttgttttat gctttgtacc
     2221 tcgcacgcca tgatgcgcga tctggccgag cagttccgcg ctaccatgac gcttcccgtt
     2281 ttgttgcagg gggaaaccag taaaggccaa ctgttgcagc agtttgtcag tgccggtaat
     2341 gcgcttcttg tggcaaccag tagtttctgg gaaggggtgg acgtgcgtgg cgatacattg
     2401 tcattggtaa ttatcgacaa attgccgttt acctcgctgg atgatccact gttaaaagcg
     2461 cgcatggaag attgtcgttt gcgcggtggc gacccgttcg atgaagtgca actaccagat
     2521 gccgtcatta ctctcaaaca gggggtaggt cgactgattc gcgacgccga cgatcgtggc
     2581 gtgctggtga tttgtgacta tcggctggtg atgcgtcctt acggcgcgac gtttctcgcc
     2641 agtctgccgc ccgcgccacg cacccgtgac attgcccgag cggttcgttt ccttgcgata
     2701 ccatcctcca ggtaatttgt tacggagtat ggtatgatgc gcgccttttt attgatctaa
     2761 gcacgaagac ccatgcgaat tctggctatc gataccgcga cagaggcctg ctctgtcgcc
     2821 ctgtggaacg acggtactgt caacgctcat tttgagcttt gccctcgtga acatactcaa
     2881 cgaatcttac cgatggtgca ggatatcctg accaccagcg gaacttccct gactgatatt
     2941 aacgctctgg cttacgggcg cggccccggt agctttaccg gcgtgcgcat tggtattggc
     3001 atcgcgcaag ggctggcgct tggcgcggaa ttaccgatga ttggcgtctc cacgctaatg
     3061 acgatggcgc aaggggcgtg gcgcaaaaac ggcgcaacac gcgtgctgtc agccattgac
     3121 gcgcgaatgg gcgaagttta ctgggccgaa tatcagcgtg atgaaaacgg tatctggcac
     3181 ggtgaagaaa ccgaagccgt actcaaaccc gaactcgttc atgaacgaat gcaacagctt
     3241 tccggcgaat gggtgacggt agggacgggc tggcaagcct ggccggatct cggtaaagag
     3301 agcgggctgg ttttgcgcga tggcgaagtg ttactccccg ctgcagaaga tatgctgccg
     3361 attgcttgtc agatgtttgc cgagggtaaa acggtggcgg tggaacatgc cgagccggtt
     3421 tatttgcgta acaacgtcgc atggaagaaa cttccgggca aagaatgaat cttaagagta
     3481 tgccctgaga aaaaaaggag tcgcatcatg gcggttcaaa agaatgttat caaaggcata
     3541 ctggcaggta cgtttgcgct aatgctgagt ggttgtgtca ctgtgccgga cgccattaaa
     3601 ggcagcagtc ccacgccgca acaagattta gttcgggtga tgagtgcgcc gcagctgtac
     3661 gttggtcagg aggcacgctt tggtggcaaa gtggttgcgg tacaaaacca gcaagggaaa
     3721 acccgcctgg aaattgctac cgtaccgctg gacagcggag ctaggccgac gctgggagaa
     3781 ccttctcgcg gtcgcattta tgccgatgtg aacggttttc tggacccggt ggatttccgt
     3841 agacaactgg ttacggtagt cgggccaatc accggtgcgg ttgacggcaa aatcggcaat
     3901 acgccctata aatttatggt gatgcaagta acgggttaca aacgttggca tttaacccag
     3961 caggtgatta tgccgcctca gccgattgat ccatggtttt atggcggtcg tggctggccc
     4021 tatggctacg gcggatgggg ctggtataat cccggccccg cgagagtaca aacagttgta
     4081 accgaataat tgcttgtttt taaagaaaaa gaaacagcgg ctggtccgct gtttctgcat
     4141 tcttacggta aagataaaaa taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt
     4201 atcaagctgg tatgatgagt taatattatg ttaacagcgt gtatatcatt tggggttgcg
     4261 atgacgacga acacgcattt tagaggtgaa gaattgaaga aggtttggct taaccgttat
     4321 cccgcggacg ttccgacgga gatcaaccct gaccgttatc aatctctggt agatatgttt
     4381 gagcagtcgg tcgcgcgcta cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg
     4441 accttccgca agctggaaga acgcagtcgc gcgtttgccg cttatttgca acaagggttg
     4501 gggctgaaga aaggcgatcg cgttgcgttg atgatgccta acttattgca atatccggtg
     4561 gcgctatttg gcattttgcg tgccgggata atcgtcgtaa acgttaaccc gttgtatacc
     4621 ccgcgtgagc ttgagcatca gcttaacgat agcggcgcat cggcgattgt tatcgtgtct
     4681 aactttgccc acactctgga aaaagtggtt gataaaaccg ccgttcagca cgtaattctg
     4741 acccgtatgg gcgatcagct atctacggca aaaggcacgg tagtcaattt cgttgttaaa
     4801 tacatcaagc gcttggtgcc gaaataccat ctgccagatg ccatttcatt tcgtagcgca
     4861 ctgcataacg gctaccggat gcagtacgtc aaacccgaac tggtgccgga agatttagcc
     4921 ttcctgcaat acaccggcgg caccacaggt gtggcgaaag gcgcgatgct gactcaccgc
     4981 aatatgctgg cgaacctgga acaggtcaac gccacctatg ggccgttgct gcatccaggt
     5041 aaagaactgg tagtaacggc actgccgctg tatcacattt ttgccctgac cattaactgc
     5101 ctgctgttta tcgaactggg tgggcagaac ctgcttatca ctaacccgcg tgatattcca
     5161 gggttggtaa aagagttagc gaaatatccg tttaccgcta tcactggcgt taacaccttg
     5221 ttcaatgcgt tgctgaacaa taaagagttc cagcagctgg atttctccag tctgcatctt
     5281 tccgcaggcg gtgggatgcc tgttcagcaa gtggtggcag aacgctgggt gaaactgacc
     5341 ggacagtatc tgctggaagg ttatggcctg accgagtgcg cgccgctggt gagcgttaac
     5401 ccgtatgata ttgattatca tagcggtagc atcggtttgc cggtgccatc gacggaagcc
     5461 aaactggtgg atgacgacga caacgaagta ccaccgggtc aaccgggtga gctttgtgtc
     5521 aaaggaccgc aggtgatgct gggttactgg cagcgtccgg atgctacaga tgagatcatc
     5581 aaaaatggct ggttacacac cggcgacatc gcggtgatgg atgaagaagg attcctgcgc
     5641 attgtcgatc gtaaaaaaga catgattctg gtttccggtt ttaacgtcta tcccaacgag
     5701 attgaagatg tcgtcatgca gcatcctggc gtacaggaag tcgcggctgt tggcgtacct
     5761 tccggctcca gtggtgaagc ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc
     5821 gaagagtcac tggtgacttt ttgccgccgt cagctcacgg gatacaaagt accgaagctg
     5881 gtggagtttc gtgatgagtt accgaaatct aacgtcggaa aaattttgcg acgagaatta
     5941 cgtgacgaag cgcgcggcaa agtggacaat aaagcctgag cgttaagtca gtcgtcagac
     6001 gccggttaat ccggcgtttt ttttgacgcc cactaaagag aaaacaattt gaattaccaa
     6061 atgattacta cggacgatgc gctggcttct ttgtgtgaag ccgtccgtgc ctttccggcg
     6121 atagccctgg atactgaatt tgttcgtacg cgcacttatt acccgcagct ggggttgatt
     6181 caacttttcg atggcgagca tctggcgcta attgatcctc tcgggatcac cgactggtca
     6241 ccgctgaaag cgatcctgcg cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa
     6301 gatctggaag tgttcctcaa tgtctttggc gaattaccac aacccttgat tgacacgcaa
     6361 atccttgctg ccttctgcgg acgcccgatg tcatggggtt tcgcttccat ggtggaagag
     6421 tattccggcg ttacgctgga caagagtgaa tcgcgcgccg actggctggc cagaccgctg
     6481 accgaacgtc agtgtgaata cgcagcggcg gatgtctggt atctgttacc gatcactgcc
     6541 aagcttatgg tagaaacgga ggcctccggc tggctacctg cggcgctgga tgaatgccgc
     6601 ctgatgcaaa tgcgtcgtca ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc
     6661 aatgccaggc agttacgcac acgccaactg gcctgtctgc aactgttagc cgactggcga
     6721 ctgcgcaagg cgcgagagtg tgatctggcg gtgaactttg tcgtgcgtga agagcatttg
     6781 tggtcggtcg cgcgttatat gccgggaagt ttaggcgaac tggacagcct gggtttatcc
     6841 ggtagcgaaa tccgctttca cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca
     6901 ttgccggaag atgccttacc gcagccgatg tttaacctga tggacatgcc tggttatcgt
     6961 aaagcgttta aagcgattaa gtcgctgatt actgacgtga gcgaaacgca taagatcagc
     7021 gccgaattgc tggcatcgcg tcggcaaatc aaccaactgc tgaactggca ccggaaactg
     7081 aaaccgcaga acaatttgcc ggagctgatt tccggctggc gtggtgagct gatggcggaa
     7141 gcattacaca atttattgca ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg
     7201 cgctcagcgc cacatccggc tttggcaaac tacaaatcca acaccaggcg tttacccttc
     7261 gcacgcgaac aacagatcaa catactttgc tggctggcac gctcttcatc gctaaaatac
     7321 tgatcccgat ggtcagcttc accttccagt attgccgttt cgcaggttcc gcatacccct
     7381 tcacgacata aacattccac tttcgcggct ttattatttt caataacctg caaaatagtc
     7441 atctcttccg gcaccacaaa ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg
     7501 ccggttttgt cttcgatagc aaattgctca aagtgcagcg tatcggcggc gatgtccaga
     7561 cgcgcagctt cacttcttac cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt
     7621 gtgccaggtt cgatatccgc caataatcgc gctaattcca gtcgtgttcc ggttgatgaa
     7681 tgatgcaaat ggactttctc agcctgcgga tgctggacta gctcatcacg aaatgcgcaa
     7741 ctttctggat ttcgcgagca gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct
     7801 gccatgtgcg acaggaaagg ggtgatacca ataccgcccg cgataaacag atgctttctg
     7861 gctgagggaa ttagcgcaaa caggttatta ggcgttgaga tcgttaaccg atcgcccact
     7921 tttacctgct gatgcaaaaa gcgggaaccg ccgcgcgagt tttcttccag tcgaactgca
     7981 atctgataac tagaggtgtt atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg
     8041 ttatcaccat cgctcatctg cactaggatg tgacttcctc cggtgaacgc aggcagcggt
     8101 ttgccatcgg ttgccaccag cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg
     8161 ctcacctgta cttcaaacat ttgatagtct gacattgctg tactccatca aaaatcagcc
     8221 cagcggcgga aaataccgcc gccgatgtca ttagtcctta aacacctgcg ccagcagatt
     8281 atggaaatgg gcgataccat gttcggaaat gccactgccg ctactgtcgg ccatgatgcg
     8341 cccctgacca cgatagccac gcgatttcag ccctttctgt acgctttcaa ccagacgtaa
     8401 atcttccgga tggaacacat cgcgatacca ctcaatcagc gatttttgct cgttggttaa
     8461 ctcttcattg gtgaagtaaa tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa
     8521 ttcataaatg accgtcatca tccctttgat cggggtgacg ttcagcatcg tgcacggcca
     8581 cagccagaaa ccgtggaatg ccgcatccgt accctcttca aatttaaacg actgttcgga
     8641 cggtttggcg aaaccgtatt gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc
     8701 aacctgtacg gagtcggaga aacctagatg cgccggacca cagtgatagc actcgagata
     8761 gttatcgaca atgttcttcc agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt
     8821 cagattgtgg acttccgggc aggcttccag cactttcgcg cccagaccgg tgtattgatt
     8881 cacttgaagt acgaaaaaaa accgggagga cattggatta ttcgggttct gatgggatta
     8941 gatttggtgg ggcttgcaag cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga
     9001 aagatagtaa aaaattgctt ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat
     9061 catttcaata acagcttcaa aaaacgttca aaaccgataa caaccaggct gtaaccaaat
     9121 gactcatatc acaaatcagc tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg
     9181 tgaagtatgt tttagctggt tcatggttgt tatacggctt tttttacatc ttgtggttct
     9241 tgtgaaggag ctacaagacc ttcctgttca taaataccat actttgacaa aatcttttta
     9301 cgagtatctg ttggtaatga ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata
     9361 ccttgcaaat cagcctggat ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca
     9421 aatcctttct ttctgaacaa ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg
     9481 acaattgcga tcagtttagt tttcaatgtg ctgcggtcag gttgagaatt tttattctct
     9541 tgattatttt tatgctcaac cattggcatg ctctgacttt ggataaacca ctctattgga
     9601 cgccctgttg cgtaggctat ttgtgctaat cgatctaaag acgggtatgt cttgccagac
     9661 aggtagtttc tgataacccc atcagaaagt ccgcacttag ctgcaaac
//
LOCUS       DADMGC010000140         9520 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.140, whole genome shotgun sequence.
ACCESSION   DADMGC010000140 DADMGC010000000
VERSION     DADMGC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.140"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(434..640)
                     /locus_tag="JNZ73_003466"
     CDS             complement(434..640)
                     /locus_tag="JNZ73_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HAY8733616.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMFFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            953..1042
                     /gene="kdpF"
                     /locus_tag="JNZ73_003467"
     CDS             953..1042
                     /gene="kdpF"
                     /locus_tag="JNZ73_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAY8733617.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1042..2715
                     /gene="kdpA"
                     /locus_tag="JNZ73_003468"
                     /pseudo
     CDS             1042..2715
                     /gene="kdpA"
                     /locus_tag="JNZ73_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
     gene            2738..4785
                     /gene="kdpB"
                     /locus_tag="JNZ73_003469"
                     /pseudo
     CDS             2738..4785
                     /gene="kdpB"
                     /locus_tag="JNZ73_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4794..5366
                     /gene="kdpC"
                     /locus_tag="JNZ73_003470"
     CDS             4794..5366
                     /gene="kdpC"
                     /locus_tag="JNZ73_003470"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HAY8733618.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAVAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5359..8038
                     /gene="kdbD"
                     /locus_tag="JNZ73_003471"
                     /pseudo
     CDS             5359..8038
                     /gene="kdbD"
                     /locus_tag="JNZ73_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
     gene            8035..8712
                     /gene="kdpE"
                     /locus_tag="JNZ73_003472"
     CDS             8035..8712
                     /gene="kdpE"
                     /locus_tag="JNZ73_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HAY8733619.1"
                     /translation="MTNVLIVEDEQAIRSFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8987..9091
                     /locus_tag="JNZ73_003473"
     CDS             8987..9091
                     /locus_tag="JNZ73_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HAY8733620.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9402..>9520
                     /locus_tag="JNZ73_003474"
     CDS             9402..>9520
                     /locus_tag="JNZ73_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY8733621.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSV"
BASE COUNT         2115 a         2394 c         2637 g         2374 t
ORIGIN      
        1 ataacatctt attggattag atatgttaac aattaattat tcttaatata tcaataaagt
       61 atatattact ctctttacta tctgttttta ttgcggtagc gatactaata acaaaattcg
      121 gaatttattt agatgctgga attagaaaaa gggatgacgg ctttctcacc ttaccctgaa
      181 gtttcacgaa agtgtgtaag gaagtatgca atctaactgg agatttctga tgatctagtc
      241 gcttagagcg acaatgaagg tgaccgcatc ctgggtagcc ctatgaaagg atactattga
      301 aacgtgatgc taaaacagtg atgaaatgaa taatacgatg gaaacgtctt ccatgttaat
      361 tatcatggaa gacgttgatg ttgaacacct aaaccagccc cctgatacga ggctggctta
      421 agcgcgcagg caatcagtaa aaatcaccgg ttgccttttc ggcctgatcc atccacaccg
      481 gtttatcgct ggttttcgac cagacgcggt gcaggtagct gtaaaagcgg gcgcggtctt
      541 tccagaaaaa catgaaaggc agcgccagaa cgcccgctgc aaccgcatat gtacggcgta
      601 aaaagataag ccatgcagga tattctctgt agagttccat acttttctcc cccttttgta
      661 tctacccggt gaatagcacc ggaaaaatga atttgtttat ctgatgaaaa tagtaccgcc
      721 ttttgtgtaa ttttaccact catccgacca cttatttttg cttattgatg gtttatttac
      781 attcacactg taattaaatt acacaaaagt taaattaata ctaaccatca gttaaatcat
      841 ggcctttgcc atttttatac tttttttaca ccccgtccgc agatttttgc gaaatctttg
      901 cagccagaat tctactcttc cggtatcact tttaggctac tggaggtgca ctgtgagtgc
      961 aggcgtgata gccggcgtgt tgctggtgtt tttattactg ggttatttgg tttatgccct
     1021 gatcaatgcg gaggcgttct gatggctgcg caagggttct tactgattgc cacgttttta
     1081 ctggtattaa tggtgctggc gcgtccttta ggcagcgggc tggcgcggct gattaatgac
     1141 attcctcttc caggtacagc gggcgttgaa cgtatactct ttcgtttacc tggcgtctct
     1201 gaccatgaga tgaactggaa gcaatatctt tgtgccattc tcggcctgaa catgctgggg
     1261 ctggcggtgc tgttttttat gttgctcggt cagcactatc tgccgcttaa tccatagcag
     1321 ttgccggggc tgtcgtggga tctggcgctg aataccgccg tcagctttgt cacgaatacc
     1381 aactggcaat cttatagcgg tgaaaccacg ttgagctatt tcagccagat ggcaggctta
     1441 acggtacaaa actttctttc tgccgccagc gggattgcgg tgatttttgc cttcatccgt
     1501 gcgtttactc gccagagcat gagcacactc gggaatgcct gggtcgatct gctacgcatc
     1561 acgttatggg tgctagtccc tgtggcgttg ttaatcgcac tgttttttat tcaacaaggt
     1621 gcgctgcaaa actttctgcc ctatcaggca gtgaataccg ttgaaggagc gcaacagctg
     1681 ttacccatgg gaccagtagc ttctcaggaa gcgatcaaga tgctcggtac taacggcggt
     1741 ggcttcttta atgccaactc gtcgcatccg tttgaaaacc caaccgcact gaccaacttc
     1801 gtgcagatgc tggcgatctt cttgatccca acagcgctgt gctttgcctt tggtgaagtg
     1861 acgggcgatc gccgccaggg gcgcatgttg ctgtgggcga tgtcagtgat ttttgtcatc
     1921 tgcgtaggcg tggttatgtg ggcagaagtt cagggtaatc ctcatctgct ggcgctcggc
     1981 gcagacagca gcatcaatat ggaaggtaaa gagagccgtt tcggcgtgct ggtcagtagc
     2041 ctgtttgcgg tcgtgactac ggcggcgtcc tgtggcgcgg tgattgcgat gcatgattcg
     2101 tttaccgctc tcggcggcat ggtgccgatg tggctaatgc aaattggtga agtggtgttc
     2161 ggcggtgtcg gttctggtct ttacggcatg atgctgtttg tcctgctggc ggtgtttatt
     2221 gccgggctga tgattggtcg tacaccggaa tatctgggta aaaaaatcga cgtacgcgag
     2281 atgaaactga ctgcactggc gattctggtt actccaacac tggtactgat gggcgcggcg
     2341 ttggcgatga tgaccgacgc cggacgtagc gccatactta accctagccc gcatggtttt
     2401 agcgaagtgc tgtacgccgt gtcgtccgcc gctaacaaca acggcagcgc ctttgccgga
     2461 ttaagcgcca actctccgtt ctggaactgt ttactggcgt tctgcatgtt tgtcggtcgc
     2521 tccggggtga ttatcccggt gatggcaatt gccggttcgc tggtgagtaa aaagagccaa
     2581 cccgccagct ccggcacgct gccaacgcac ggcccgctgt ttgttggcct gttaatcggc
     2641 accgtgttgc tggttggcgc actgaccttt atccctgccc tggcgcttgg tccggtggcg
     2701 gaatatctct cctgatgata ttgagtgagc actgaatatg agtcgtaaac aactggcgct
     2761 attcgaacca acacttgtcg ttcaggcgct gaaagaagcg gtgaaaaaat taaacccgca
     2821 ggcgcaatgg cgcaatccgg tgatgtttat cgtctggatc ggcagtctgc tgaccacctg
     2881 tattagcatc gcgatggcaa gcggtgcgat gcctggcaat gcgctgttta gcgcggcaat
     2941 tagcggctgg ctgtgggtca ccgtactgtt cgctaatttc gccgaggcgc tggcagaagg
     3001 ccgcagtaaa gcgcaggcca acagtcttaa aggggtgaaa aaaactgcct ttgcccgcaa
     3061 gctgcgtgag ccgaaatatg gcgctgcggc ggacaaagtt cctgccgatc aactgcgtaa
     3121 aggtgacatc gtgctggtag aagctggcga tattatcccc tgcgatggtg aagtcattga
     3181 aggcggcgca tcggtcgatg aaagcgccat caccggggaa tcggcaccgg tgatccgtga
     3241 atccggcggc gattttgcct ccgtcaccgg cggcacgcgt attctttctg actggctggt
     3301 gattgagtgt agcgttaacc ccggtgagac atttctggat cggatgatcg cgatggtgga
     3361 aggcgcacag cgacgcaaaa caccgaacga gattgccctg actatcctgc tgattgctct
     3421 gaccatcgtc tttttactgg caaccgccac gctgtggccg ttttccgcgt ggggcggtaa
     3481 tgccgtgagt gtaacggtac tggtggcgct gctggtctgt ctgatcccaa ccactattgg
     3541 tggcctgttg tcagcgatcg gcgtcgccgg gatgagtcgg atgctgggcg cgaatgtgat
     3601 tgccaccagc ggacgtgcag ttgaagcggc aggtgacgtt gacgttctgc tactggataa
     3661 aaccggcacc atcacactcg gtaaccgtca ggcgtcggag tttatccccg cgcagggcgt
     3721 ggatgaaaaa acgctggctg acgccgcaca actggcttcg ctggctgatg aaacgccgga
     3781 aggtcgcagt attgtgatcc tcgccaagca acgttttaac ctgcgcgagc gcgatgtgca
     3841 gtcgctccat gccacctttg taccgtttac tgcgcaaagc cggatgagcg ggatcaacat
     3901 cgacaaccgc atgatccgta aaggttctgt cgatgccatt cgccgccata ttgaggctaa
     3961 cggcggtcac ttccctgccg atgttgatca aaaagtcgat caggttgcgc gtcaaggagc
     4021 cacgccgctg gtggtggtgg aaggttctcg tgtgctgggc gttattgcgc tgaaagatat
     4081 cgtcaaaggc ggtattaaag agcgcttcgc ccagctgcgc aaatgggcat taaaacggtg
     4141 atgattaccg gcgataaccg tctgactgcc gccgcgatag ctgcggaagc gggtgtcgat
     4201 gattttctcg ccgaagcgac accggaggcc aagctggcat tgatccgtca gtatcaggcg
     4261 gaaggtcgtt tggtagcgat gaccggcgac agcaccaacg atgctccggc gctggcgcag
     4321 gcagatgtcg cggtggcgat gaactccggc acccaggcgg cgaaagaggc gggcaatatg
     4381 gtggatctcg actctaaccc gaccaagttg atcgaggtgg tgcacattgg caaacagatg
     4441 ctgatgaccc gtggctcgct gaccaccttc agcattgcca acgatgtggc gaaatacttc
     4501 accattattc cggcggcgtt cgcggcaaca tatccgcagt taaatgcgct gaacatcatg
     4561 cgcctgcatt cgcccgactc cgccattctc agtgcggtca ttttcaacgc cttgattatc
     4621 gtctttttga ttcctctggc gttaaaaggc gtgagttata aaccgcttac cgcttctgcc
     4681 atgttgcgcc gtaacttatg gatttacggt ctgggtgggc tgctggtgcc gtttattggt
     4741 atcaaagtca ttgatttact gctaaccgtt tgtagtctgg tgtgaggttt accatgagtg
     4801 gattacgtcc ggcattatcg acatttctct ttctgttatt gattactggc ggcgtttacc
     4861 cgctgctgac caccgcactg gggcaatggt ggtatccctg gcaggccaac ggttcgttga
     4921 ttcgtgaagg tgatacggtg cgcggttcgg cattaatcgg gcaaaatttt accggcaacg
     4981 gctattttca tggtcgcccg tcggcaacag cagaaatgcc ctataatcca caggcttctg
     5041 gcgggagcaa tctggcagtc agtaacccgg agctggataa acagatagcc gcacgcgttg
     5101 ccgcattacg ggccgctaac ccggacgcca gcacgaacgt tccggttgaa ctggtgacgg
     5161 cgtcggcaag cggactggac aataacatca ccccgcaagc ggtggcctgg cagatcccac
     5221 gcatcgcaaa agcgcgtaat ctcagcgttg aacagctcac gcagctgatc gcaaaataca
     5281 gccaacaacc gctggtgaaa tatatcggcc agccggtcgt caacattgtc gaactcaatc
     5341 tggcactgga taagcttgat gaataacgaa cccttacgtc ccgaccccga ccgtctgctg
     5401 gaacaaactg ccgcgccgca tcgggggaag ctgaaagttt tcttcagtgc ctgtgcaggc
     5461 gtcgggaaga cctgggcgat gctggcagaa gcccagcgac tgcgggcgca agggctggat
     5521 attgtggttg gtgtggtaga aacccatggg cggaaagata ccgccgccat gcttgaaggg
     5581 ctggctattc tgcctctgaa acgccaggcg taccgtgggc ggcatatcag cgagtttgat
     5641 ctcgatgccg ccctcgcccg ccgtccggcg ctgatcttaa tggacgaact ggcgcacagt
     5701 aatgcgccag gttctcgtca tcctaaacgc tggcaggata tcgaagaact gctggaagct
     5761 ggcattgatg ttttcacaac cgtcaacgtt cagcatctgg aaagtctgaa tgatgtggtc
     5821 agcggcgtca ccggaattca ggtacgggaa accgtgcccg atcctttttt cgatgccgcc
     5881 gacgacgtgg tgctggtgga cttgcccccg gacgatctgc gccagcggct gaaagaaggc
     5941 aacgtctata ttgccgggca ggcggagcgc gccattgaac attttttccg caaaggcaat
     6001 ctgatcgccc tgcgcgaact ggcactgcgc cgtactgccg atcgcgttga tgagcaaatg
     6061 cgcgcctggc gggggcatcc tggcgaagag aaagtctggc acacgcgcga cgcgatcctt
     6121 ttatgcatcg gccataacac cggcagcgaa aactggtccg cgcggcggcg cggctggcgt
     6181 cacggctggg gagcgtctgg cacgcggtgt atgttgaacc ccctgccctg catcgcttac
     6241 cggagaaaaa acgtcgggca attctcagcg ccttacgtct ggcgcaggag ctgggcgcgg
     6301 agacggcaac actttctgat ccagcagaag agaaagcggt agtgcgttat gcccgtgaac
     6361 ataatctcgg caagattatt ctcggtcgcc cagcctcgcg ccgctggtgg cgtcgggaaa
     6421 catttgctga ccgactggcg cgcatcgccc ccgatctcga tcaggtgctg gtcgcgcttg
     6481 atgaaccacc cgcccgcacg attaacaacg cgccggatag ccgctcattt aaagacaagt
     6541 ggcgggtaca gattcaggga tgcgtggttg ccgccgcgtt atgcgccgtt atcaccttaa
     6601 ttgccatgca gtggctgatg gcgtttgatg ccgccaacct ggtgatgctg tatctgcttg
     6661 gcgtggtggt ggcgctattt tatggacgct ggccttcggt ggttgccacc gtcattaatg
     6721 tggtgagttt cgatctcttt tttatcgccc cacgcggcac gctcgccgtc tctgatgtgc
     6781 aatatctgct gaccttcgcg gtgatgttaa ccgtcgggct ggtgatcggg aaccttactg
     6841 ctggcgtgcg ttatcaggcg cgggtagccc gttaccgcga gcaacgcaca cggcacttat
     6901 atgaaatgtc gaaagctctg gcggtgggcc gcagtccgca ggacatcgcc gccaccagcg
     6961 aacaatttat tgcctctacg tttcatgccc gcagtcaggt gttgttgccc gatgacaacg
     7021 gtaaattgca gccgttaaca catccgcaag gaatgacgcc atgggacgat gccatcgcgc
     7081 agtggagttt tgataaaggc ctgcctgcgg gcgcgggcac cgacacgtta cccggtgtac
     7141 cgtaccagat tttgccgcta aaaagcggcg agaaaaccta cggctggtgg tggtggagcc
     7201 ggggaatctg cgccagttga tgatcccgga acagcagcgc ctgctggaga cttttacgct
     7261 gttagtcgcc aatgcccttg agcggctgac gctaaccgcc agcgaagaac aggcgcggat
     7321 ggcaagcgaa cgtgaacaga tccgcaacgc cctgctggca gcgctttcgc atgatttacg
     7381 cacgccgctt acggtgctgt ttggtcaggc agaaatctta acgctcgatc tggcaagcga
     7441 aggatcaccc cacgcccgcc aggccagcga gattcgtcag catgtgctga acactacccg
     7501 actggtgaat aatctactgg atatggcgcg aattcagtcc ggcggcttta atttgaagaa
     7561 agagtggtta acgctggaag aagtagtcgg cagcgcgctg caaatgctgg aaccgggttt
     7621 atcgtcgccc atcaatcttt ctctgccaga accgctgacc ttaatccacg ttgacgggcc
     7681 actctttgaa cgggtgctga ttaatctgct ggagaacgcg gtgaaatatg cgggtgcgca
     7741 ggccgaaatt ggtatcgatg cccacgttga gggcgaaaat ctacaactgg atgtctggga
     7801 taacggcccc ggtcttccgc caggccagga gcagacgata tttgataagt ttgctcgcgg
     7861 gaataaagag tcggcggtgc cgggtgtcgg gcttgggttg gcaatttgtc gggcgattgt
     7921 ggatgtacac ggcggcacca ttaccgcgtt caaccgaccg gaaggtggtg cctgttttcg
     7981 tgttacactt ccccagcaaa ccgctcctga acttgaagac tttcatgagg atatgtgaca
     8041 aacgttctga ttgttgaaga tgaacaggct attcgtagct ttctgcgcac ggcgctggag
     8101 ggcgacggga tgcgcgtctt tgaggccgaa actctgcaac gcggcttgct ggaagcggca
     8161 acccgtaagc cagatttgat tattctcgat ctcggcctgc ccgatggtga tgggattgag
     8221 tttatccgcg acctgcgcca gtggagcccg gtgccggtga ttgtgctttc cgcacgcagc
     8281 gaagagagcg acaagatcgc cgcgctggat gccggagcgg atgattatct gagtaagccg
     8341 tttggcattg gcgaattgca ggcccgtctg cgcgtcgctt tacgccgcca ctccgccacc
     8401 gccgcgcccg atccgctggt gaaattttcc gatgttaccg tcgatttagc cgcccgcgtg
     8461 attcaccggg gtgaggaaga ggtgcatctc acaccaattg agttccgcct gctagcggtg
     8521 ctgctcaaca atgccggaaa agtactcacc cagcgccagc tccttaacca ggtgtggggg
     8581 ccaaacgcgg tcgaacacag tcactatttg cgtatttata tgggacatct gcgacaaaaa
     8641 ctggaacagg atccggcccg gcctcgccac ttcattactg aaaccggtat tgggtatcgg
     8701 tttatgcttt gaattgttat tcaataaatt cgcttaaata gtgattaatt tattattaaa
     8761 cctgcgccga atattattat ttggcaggtt taatttctta acgcaaatat aaaacacgaa
     8821 attacaacat ttaagtaaca aaacagcatc taatatggcg atattcataa ttaatgattt
     8881 attatctggg gtatgatcgt tttttgttga tcttcttcac agattatagc catttcatgg
     8941 atagaataac tctaccttca actgacacag caagcggtaa aggtaaatgg aaaataataa
     9001 ccgcttaatg cctcatataa ggcggacaac ccatatcatg aagtttgccc atcgtaatag
     9061 ttttgacttt catttcttta atgcccgcta gtcttctgac taaagggcac cccaacgtac
     9121 aggtctccct gactttaagc attacaggtt aatacctgta ttcctcggtg ctcatatact
     9181 gctaaccctt ttaaactcta aataattcga gtcgcagcac ttgcaacttg aggtatgacg
     9241 agtatagcca gttaccgggc tggtctgggt tattgcatct gcaaaaagca aactactgat
     9301 ttatttatca gcggtggagc tttgcttttt ttccggcgtg atcgatttct cctttgagaa
     9361 attgaggacc tgctattacc tgaaataaag agatgaataa aatgtcagaa ttaaaaattg
     9421 ccgttagccg ttcttgcccg gattgttttt ccactcatcg tgcatgcgtg aatattgacg
     9481 aaagtaatta tattgacgtt gccgccatta ttttatcagt
//
LOCUS       DADMGC010000141         9497 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.141, whole genome shotgun sequence.
ACCESSION   DADMGC010000141 DADMGC010000000
VERSION     DADMGC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.141"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..399)
                     /gene="ompG"
                     /locus_tag="JNZ73_003475"
     CDS             complement(<1..399)
                     /gene="ompG"
                     /locus_tag="JNZ73_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HAY8733622.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRW"
     gene            complement(444..1526)
                     /locus_tag="JNZ73_003476"
     CDS             complement(444..1526)
                     /locus_tag="JNZ73_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8733623.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTILEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(1540..2199)
                     /gene="pgmB"
                     /locus_tag="JNZ73_003477"
     CDS             complement(1540..2199)
                     /gene="pgmB"
                     /locus_tag="JNZ73_003477"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAY8733624.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(2196..4463)
                     /gene="ycjT"
                     /locus_tag="JNZ73_003478"
     CDS             complement(2196..4463)
                     /gene="ycjT"
                     /locus_tag="JNZ73_003478"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HAY8733625.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     ARGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(4460..5066)
                     /locus_tag="JNZ73_003479"
                     /pseudo
     CDS             complement(4460..5066)
                     /locus_tag="JNZ73_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(5114..5860)
                     /locus_tag="JNZ73_003480"
                     /pseudo
     CDS             complement(5114..5860)
                     /locus_tag="JNZ73_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
     gene            complement(5891..6733)
                     /locus_tag="JNZ73_003481"
     CDS             complement(5891..6733)
                     /locus_tag="JNZ73_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY8733626.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVTWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(6720..7601)
                     /locus_tag="JNZ73_003482"
     CDS             complement(6720..7601)
                     /locus_tag="JNZ73_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY8733627.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLLAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(7622..8905)
                     /locus_tag="JNZ73_003483"
                     /pseudo
     CDS             complement(7622..8905)
                     /locus_tag="JNZ73_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(8919..>9497)
                     /locus_tag="JNZ73_003484"
     CDS             complement(8919..>9497)
                     /locus_tag="JNZ73_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAY8733628.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMDALSRRESSDEERCARFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         2266 a         2647 c         2324 g         2260 t
ORIGIN      
        1 ccagcgctgc atattcgccg tgtctttgcc cggttcatca acgtagtgat aaccataatt
       61 acggaaaccg ccggtcaggc cgaaactgaa atcatcgctt tcgaggaact gataatgcac
      121 ctccagctcc gggcgatcaa accacgttcc acgtttaccc gcgctataat ctaccggccc
      181 ttcctgataa taggccagag caattctcca cggcccgttg gcggcattaa aatagactga
      241 aggctccgcc agcccatcca tatcttcgcc ataaccctcg acgttttcta tttcgtacat
      301 cgcaccgata ttaaagtgcc agtcgttctt ttcctcggcc tgtgcgcagg ccattcccgc
      361 acacatcacc agtgcggtac agggtaataa ctttttcatt attgttcctt gtattatcct
      421 aggggaatgc cttccccctg tatttagcga attgctattt ccgtttctgc atcaaagaaa
      481 tgacatttcg tcatatcaaa atgaatagtg atattttctc ctgcatgata atcatttaac
      541 gcaccagcac ggaccactaa ctcgtgcccc ccaaccgtgg tgtagagcat aaattccgca
      601 ccggttaatt ctgccacgct aattttggcg gaaatgttat tttcctcttg cgcgtccgga
      661 tgaatatctt ccgggcgtat tcccatcacg atgggcttat gcaaactttc ctgtgttttc
      721 agaaccgcta atttctcttc gagaatggtt aatttaagcg tttccgtaac gaatttatcg
      781 ccatcgatcg tgccgcgaat aaaattcatc gctggtgatc caataaatcc ggcaacaaac
      841 atattcgcgg gttggttata aacggttttc ggcgcaccta cttgctgaac aatcccgtct
      901 ttcataatca caatccgcgt cgccatggtc atcgcttcgg tctgatcgtg ggtcacgtag
      961 atcatggtgg tgttcagttt ctgatgcagc ttgctgatct ctgcacgcat ttgtacgcgc
     1021 agcttggcat caaggttaga gagcggttca tccattaaaa acacgcccgc ttcgcgcaca
     1081 atcgcccgcc cgagcgccac acgctggcgt tgcccgccgg aaagcgcgcc cggcttgcgt
     1141 ttcaggtact cacgcaggcc gagaatttgc gccgcccagt tcacccgctc atcaatcacc
     1201 tctttggcga ttttttgcat cttcaggcca aacgccatgt tgtcgtaaac cgtcatatgc
     1261 ggatacaacg cgtagttctg gaacaccatc gcgatattgc gtgctttggc tggaacgtca
     1321 ttcattcgtt tgccgtcgat caacagatcg ccgccgctga tctcctcaag cccggcaatc
     1381 atgcgcaggg tggtcgattt accgcagccc gacgggccga caaatacgat gaactcttta
     1441 tcggcaattt ccaggttgaa gtccttcacc acatgcacct ggttatcgta gattttttga
     1501 atatgttgta acgaaagctg agccatgttg attcctttgc tatacgtttt gccagaaggc
     1561 cgataaccgc gaccaggtca gggagtccgt tgagggcaac agtaattgcg ccccggttaa
     1621 gcccgcaccg atccccaccg agcgcatacc gctggcgtta atggcgtcaa tgcccgcctg
     1681 cgcatcttca atgccgatac atgcctgcgg cggcacgccc agcccggcac aggcagcgag
     1741 aaagatttcc ggatccggtt tcgagttttt aagttgggaa gcatccgcgc agaaggtgaa
     1801 aaactcgcgc agctccagcg ccgctaaaat cgtcggcgca ttcagggaga cggaagccag
     1861 ccctaccgga atctgctgtg cacggagctc tgccagcaaa ttgcgaatac cgggcagcac
     1921 agcattaacc gtcagctcgc gcagtgagtg gacgtaaagc agatttttgc gatacgccag
     1981 ttgcgcccgc tcctgcgggt taaagtcgcc ctctttgccc ccgtgttgca gaatgcgccg
     2041 cagagactca tcgcggctga tcccttttag agattcgtta aactgcgcat caatactgat
     2101 gccaatttca gcggcaatct gctgccacgc ctggaaatgc agatgcgcgg tatcggtgat
     2161 tacaccatcc agatcgaaaa ttaccccttg cagtttcatt catcctcctg atgcgtggta
     2221 gcggtcccat tgaagggcaa aataaaatca cccaaacaga aaacagattc ttcagccacg
     2281 gatataagct gaccgttcaa acgcagtgaa acgggcgcag aagttcgaat cgcaatacgc
     2341 tgcgcgtcga gagtgacctg taattcgcag ccctgccaga acagagggaa agacaactgt
     2401 tgccactgct caggtaacgc cggatttaga tgcaattcac cgtcacgcac gctcaccccg
     2461 gcaaaaccct gaatcgcccc cagccagata gcgccagttg cggcagcatg gataccatca
     2521 tcacaactat gcggatcagc accaagatcg atttcagtcc cctcgcgcca gaactgataa
     2581 ctttgggtca gcaggccaca gcgtgcagca acaatgccgt ggattgcttt acttaatgac
     2641 gagtcgtgaa tagtgcgcgg ttcataaaat tgcagattgg caagacacga tgccgatgag
     2701 aactgctctg gcagcatgta attgagcatc accacatcag cttgtttgag gatctgcatc
     2761 tcgttcactt ctgcgcgtga ataatccagc agaatggttt gcttccccgc cgccgctttg
     2821 tatttcgcca gattaatcgc cggcttagtc ataaacgaat catcctgcgg caaaacaccg
     2881 tcgggctgaa tttctggcag ccgcagttct ttaaggaaca tttcggcacg atgaataaac
     2941 gcgtcgtcac tacagccaaa ctggcgggca atactcagcg cctgctggac gttgtaatac
     3001 accatatagc tggtgaaggc gttattattg acatgttcgg tatattcgtc tggcccaata
     3061 acatcatgaa tttccagacg gtcgttaacc ctcaccgcgc ggctaatcca gaactttgca
     3121 gtttccagaa gtagcgccat gccttcatgc gcaatgaaac tttcatcccc cgtggtctgc
     3181 cagtattgaa taaccgccca ggcgatatcg gccaccagat gatgttccgc ctgcgccgag
     3241 gccacttttt gccgcagccc ggtgcgaatg ttaatggcgg caaattccgg cgtctcttct
     3301 tcgccgctgc gcgcgctttc ccacggaaat agcgcgccct gccagccgtt gcgtcgcgct
     3361 ttctcctgcg cgcctggcaa gttgtgccag cgataacgca gtaaacttcg ggcaaccgtc
     3421 ggatcgctaa acagatggaa cggcagcaaa aatacttctg tatcccagaa aacgtggcct
     3481 ttgtagcctt cgccggttaa gccttttgcc gcaatactgc tgcgctcgcc gtgagccggc
     3541 gtcatgatgc gcagatgata aagcgcataa tctaacgctt gctgatcgtg cgcatcgccg
     3601 ccatttaccg tgatacgacg tttctgccac cattgacgcc agttttctgt tgatgctgca
     3661 agatgttggt cgtaactctg ttgcgcgcac atttcaagct ggcgaagcga cacgctgccc
     3721 cactcgtcta aagcagcttg cctgtcatcc cgccagtcga tccacaccag tttttgcaac
     3781 gtcactgtct cgcctgcatg aagctgcgca ctggtatgtt gcagtaaacg gcgctctttg
     3841 gcggtatagc attgctgcac atcaccgctc acctggcagc aacaactgat ggccacatca
     3901 ctgcgcccat cctgggtggt gtagatcccc tgcatcaaat gctgaccaaa cacccgcacc
     3961 tgggtttcgt cgagatgctg gcgaccgtgg ttggtttgcg tggcgtcaat gcctgttgaa
     4021 atcagcactg acgcgtcggc gtccagtggc ataatagtga tttccagcgc aatgagcggc
     4081 agttggtctg ccgaaacaaa gcgacggctg gcgatggtgt aacctgcgcc gttgctggta
     4141 cgccagacaa cgctacggcg taattctcca ctggcaaagt caagttcacg ctgccaggct
     4201 tcgcgggata acgagaaaac ctcaccattt atggcaatct ccatccccaa gatatcaggc
     4261 agattcacca gttcgttgat ttcacctttt cccgcccgat gatacagccc cgccagatac
     4321 atccctcgcg cctggcgcgt gtaatcttct tcatggctgg cgcgaagccc aagatagccg
     4381 ttcccctgcg ccatcagcga tgcatacttg ttcagggtat gctggctgaa atggggttct
     4441 gataacgtta ctggcctggt cataattcca cacgcgtccc tgtttctgcg gattgatata
     4501 acgccgccac cagttgctgg atgatgtacc cctgctcggc gtcggctatc atcacgggct
     4561 tgccctgtac gtgattgata aaggcttcca tgctgcgcaa atggcggttg tcgtctgcca
     4621 tttcccgttg catcagcgtc attaattcac cgttgttatc ggtgtagata tgtgctggaa
     4681 acagcgtcgc accggcttta tcaccacaaa agctgacgtt cataatcaac tgttcgcgga
     4741 tgttgagtgc aaatgacgtt tccagccaca gaatgccgcc gttatgaaat tcaatggtgc
     4801 caaacagcga atcttcgacg ctgtaagttg ccggatccca ctcgccaaat tgaccacagc
     4861 tcttttgcgt gccgatcttt tgaaaactat gcgcattcac gcttttcacc gccggaaagc
     4921 ccagcacata catcgcagca tccagcatat gaatgccgat gtcgatccag ttctttattg
     4981 gtaaagacac cccagccggg aacgccgcag cgacgcaggg cgcgggcggt ggtaacgtaa
     5041 atctctccca aaacgccgtt ggtcacctgt tcacgcagct gttgcgtatc gagcgcaaac
     5101 gatggtgaaa gtcgtaggcc aggccgcgca gcgccgattg cagcgcgtcg gcgtaaccgc
     5161 tggtttcgat aatcacatcg gccccctgct tgccggtcag cgttttgatc tctttgccta
     5221 cgtcagtgcc aatggggtta aggcagaaat cagcaccgtg gcgacgggca atatcacagc
     5281 gatgggcgat aggatcgacg ccaattacca ccgatgcgcc agcgcgtttc gccagttgga
     5341 tggcaatttg accgatcgcg ccaagcccta ccaccacgac aaaatcccct acgcgtacgt
     5401 tggcatcgcg cacgccgctc atggcaaact gcgccgggtc gtaacagacg gcgtttttcc
     5461 aggagctgcc ttgcggcatt ttgcgcagct tgtagttatt cactgcgtta atgatgaccg
     5521 tctcggagag cgggccgtag ccgcatacgc tgtcgcccac cgcgtagtct gtaacgtcgc
     5581 tgccgcactc gataatgtcg ccaaccacca tgttgccaag ctggaatttg ccaaactcaa
     5641 tgccgcgcgg cgcatctgcc ggacgcgggg tgaacatctg ccactcgccg ttaaagtctt
     5701 catcgataaa cgggctggcg gcgcggaagt cgaccacttc cgttccgtgt ttcggtgcgc
     5761 cgaaacgcac gcggatcttc acttcattgg ctaaaatcgc ccgatcttca tactcaacca
     5821 gcgcagcaac acgcggtgct gtggctacta actttttcat gtaaaactcc ttgtaaagaa
     5881 actggccgct ttagcccttc acgccaccgg cggtcaaacc actcttaata aaacgttccg
     5941 aaagcgcata cataatcacc accggcaatg cggtcaccag cgaggccgcc atcatccgtc
     6001 cccagatgta atcaggcgta ctgaacagcg cgttcaggcc cactggtaag gtgaaattgc
     6061 tggcgctgga gaggaaaatc gacgcaaaca gatagtcgtt ccacgtcacc ataaagcagt
     6121 agacgaacac cgatatcagc ccggacatcg ccaggggcac ggtgatacgg aagatgattt
     6181 gcagacggtt gaggccatcc atcatcgccg cttcttcgat ctcatcaggg atggtgtcga
     6241 agtaactttt cagcataaac acggcggtgg gcagggtttg cgtcaccatg gtaatgatca
     6301 gcgccatctc ggtgtcgtaa ataccaagcg cggtgatgat tttgaacaga ggcaccacca
     6361 gcaaaatgcc agagaacata tacaccgtgt aaaagctggc gttgatggtc atccggcctt
     6421 taaagcgcag gcgggaaagc gcgtaagctc cgagaatgcc gagaaatacc gccaccacgg
     6481 aggaaaccac cgacaccacc atgctgttac ggaagtagtc gacaaacggg aaaatcatcg
     6541 ggttaaaaat gtcgacgtaa tgctccagcg tccactgttg cggcagcagc gtaggatgca
     6601 gcgatatcgc ctctttcgcg cttttgaacg aggtcatcag catcacaaaa aacggaaaca
     6661 gcgtgatgat gagaaacagc gccagcccgc agtaaaaacc gatacgactg agcgtgcgtt
     6721 tatttgttgc cattgaggtt cacccgtttc ctggtcagca gaatgacagc gaaaataatg
     6781 atgaagagca ccaccgagat cgccgccgct ttccctaagt cattaaaggc aaaggcggtt
     6841 ttgtagagat agacaccaag aatatcgacc ttggttgtca gcagataaac atcagcgaac
     6901 atgtagaaca tccagatggt gcgcagggtc accaccgtcg ccaagaccgg cataattgcg
     6961 ggcagcgtga cgatgcgaaa ccgctgccag gcattagcgc catccatctc tgccgcttcg
     7021 tacagcgatt tgtcgatggt ctgcaaaatc gccagaaacg agataaaggc atacgggaag
     7081 tagcgccaga tggcgagcag caccaccagc acaaagctac tgcccggatt atcaaaccac
     7141 agcggtgcct gctcatagag atgcagaaga tcgacgccga ggtagttcac aatgccgtag
     7201 ccgttgttga acatgtattt ccaggcaaac accagcgaaa tggacggcgt tacgtaggag
     7261 aggatcacca gcgaacgcgc ggttttgcgc aggcggaact cgcggttgaa aaacatcgcc
     7321 acggccagcc cgagcacggt gctgcccgcc accaccagcg ccgtatacca gaccgtcatc
     7381 cacagcgagt gccagaagcc gggatcggag agaatgcgca catagttact caccccaaca
     7441 aacgttgact cgatgttggg attgagcggc agacgtaaaa aactgatttc gatattcgac
     7501 accatcggcc acgccaccag accgcccagc agtaataagc tgggcgcgag aagcagcagc
     7561 gcaaacggca tatcggaacg acctgaaaaa agcctgttca ttgcggattc ctgtcctggg
     7621 tttagtgctg ttcgatcagt tcatccagct ttttctggct cgcttgcagc gtgccaggga
     7681 gatcggcttt acccacggtg acgttatgca ccatggagct caccacacca gaacccgtca
     7741 catcgcccat gcgggtaaag tttttatccc ctactgcgcc aaagacctga atatttggca
     7801 gttcaccgat tagctgattc ggtagttcac ccagcgcctt aataacgtcg ttgtctttcc
     7861 aagtggcggt agtgacaacc gctttattca ccggcagcgc agcacctggc gacatcatca
     7921 cccagtcggc aatgttgtcc gcctgctcca taaaggtgac aaatttttct gctgcttcgg
     7981 tcttttgtcc ggcggtaatg gtcagcgagg tcaacatgcc gtagaccgca gagtttttct
     8041 cggttggcac cacgaaaccg acgtttttcg ggtcgccttc tttaatcaca gccggaagga
     8101 tataggtgga gtaaatcgcc atcggcgcgg tgccgttcat aaaggcgtct ttcacttcca
     8161 tgatgtcgtt agaacccggc atggtgttgg cagcaaggtc gcggtaatag gtcagcgcct
     8221 gcatcatctc tggtgtatca agggtgattt tgccttcggc gttaaagacg ttggcctagt
     8281 tggataacgc aaactgggag aaggattgtt ccgtcagcac gctttctgcc gtaggcagcg
     8341 caataccgta ttttttattc gccgggtcat tcagtttctg tgcaacgtcc agcagctgtt
     8401 gccagttttt cggctcctcc agccccgctt ttgccagcac atctttgcga taccagatgc
     8461 cgccaatcca ggcgctgaca ggaacaccgg tccatgcgct accatcttcg gtacgcacaa
     8521 tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt gctgatgacc gtggcaaccg
     8581 ctttgcgatc gataagctgc tctttatcca taactttggc gtagtcatgg ctggtttcga
     8641 tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac tttggtgtta taagcgtctt
     8701 cttccacggg cacttgttta acggtgatac ctgggttttc tttttcaaaa cgggcaatca
     8761 atttgctgat aactgcctga cgctcctgct ccaccgaaga atgcataaat tcgatggata
     8821 catgggtttt attttcttct ttacagcctg aaatcagggc gcaggaaacc agtgctgata
     8881 acagcacgat ttttgattta atcatgagat gttccttttt atttaatcca cataacctgc
     8941 caagggcgca gcgttatttc attacccaat atatcaactt cactaatcag atcccgacca
     9001 tgcagatcag taatatttac atgctgaata tttttactga cattaaacaa tccagtcaga
     9061 caattaccat cagcgttact tctttgaata cgcattacgg atgaattaac cgtatcgatg
     9121 gtaaaattat tatccggatg aaactcgtta tggctgcgac gaagtgtaat taaacgcgac
     9181 aactcatgat ataccgcatg ccttaatgta gcttcatcgt tcagttctcg tgttatctct
     9241 tcactgtgat attttttacg gttaatcgca cggttatatc cgagtttttc gacacctgcg
     9301 taatcattac gcgagccaag aatactttga atatatatcg cgggtacacc ggggaaactt
     9361 aacaaaatcg catgggcaag gataaacctg gcgcaacgtt cttcatcgct actctcgcgg
     9421 cggcttaacg catccatata ggtgacattc atctcatagg gactgcgcgt gccgtcggga
     9481 ttatttttcc agtttac
//
LOCUS       DADMGC010000142         9240 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.142, whole genome shotgun sequence.
ACCESSION   DADMGC010000142 DADMGC010000000
VERSION     DADMGC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.142"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="JNZ73_003485"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="JNZ73_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HAY8733629.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="JNZ73_003486"
     CDS             1828..3147
                     /locus_tag="JNZ73_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HAY8733630.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3512..4657
                     /locus_tag="JNZ73_003487"
     CDS             3512..4657
                     /locus_tag="JNZ73_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY8733631.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4582..5199
                     /locus_tag="JNZ73_003488"
                     /pseudo
     CDS             4582..5199
                     /locus_tag="JNZ73_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5457..5789
                     /gene="ddpX"
                     /locus_tag="JNZ73_003489"
                     /pseudo
     CDS             5457..5789
                     /gene="ddpX"
                     /locus_tag="JNZ73_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5803..6699
                     /locus_tag="JNZ73_003490"
     CDS             5803..6699
                     /locus_tag="JNZ73_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HAY8733632.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6696..7682
                     /locus_tag="JNZ73_003491"
     CDS             6696..7682
                     /locus_tag="JNZ73_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8733633.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="JNZ73_003492"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="JNZ73_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="JNZ73_003493"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="JNZ73_003493"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HAY8733634.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2255 a         2256 c         2321 g         2408 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acacttagtt aaacagctat aaatccctaa taaaaatgta tgaatatgaa
     3301 aaaccatacg atattcgctg tcaccgaaaa ataattacga tgggctatac tcttctgaag
     3361 aacagtagcg tagattgaaa ctgtcttaag tcgcttattt cttttcagta tatcttcata
     3421 tttcaggaga atatggtgca gttagtcgat ctcgcacgtt gcgtttcgtt ttgattccgt
     3481 ctgatttccc agccttataa gggtgtgaat tatggagatg tattttaaaa gaatgaaaga
     3541 tgagtggacc ggacttgtcg aacaggcaga tcctctcatt cgtgctaaag ccgcagaaat
     3601 tgcccttgcg catgctcatt atctgagtat cgagttttat cgaattgtcc gcatcgaccc
     3661 gcatgccgaa gaattcttga gtaatgaaca agttgagcgg cagttgaaaa gtgcgatgga
     3721 acgctggatt attaacgtgc tttctgccca ggttgacgat gtcgaaaggc taatacaaat
     3781 ccagcatacc gtcgcggaag tgcatgcccg cataggaatt ccggtagaaa ttgtcgagat
     3841 ggggtttcgg gtgctgaaaa aaatcctcta tccggtaatc ttctcttcgg attattccgc
     3901 cgcagaaaaa cttcaggtct accatttctc gattaacagt attgatatcg cgatggaagt
     3961 gatgacccgc gcgtttacct ttagtgacag tagtgcctca aaggaagatg aaaactatcg
     4021 tatcttctcg ttactggaaa acgccgaaga agaaaaagaa cggcaaatag cctcaatact
     4081 ttcatgggaa atagatatta tctataaagt cctgctggat tctgatttag gcagcagttt
     4141 gcctttaagc caggctgatt ttggcctgtg gtttaaccat aaaggtcgac attattttag
     4201 cggcattgcc gaagtgggcc atatctcccg tctgattcag gatttcgacg gtattttcaa
     4261 tcaaaccatg cgtaacacca ggattttgaa taacagaagt ttgcgggtga aatttctatt
     4321 acagataaga aataccgtat cgcagattat taccttgctg cgtgaattgt ttgaagaagt
     4381 atcgcgccac gaagtcggta tggatgtact gacgaaatta cttaaccgcc gtttcctgcc
     4441 gactatcttc aaacgcgaaa ttgcccatgc caaccggacc ggtacaccgc tgtcagtgct
     4501 gattattgac gttgataaat tcaaagagat caacgatacg tggggccata acactggcga
     4561 tgaaattctg cgtaaagtct cattcctctc gcagaagaga ttggtgaaat cgaaaatatt
     4621 gggcgctggg tcatcgcgga agcttgccgt cagttagcag aatggcgtag ccagaatatt
     4681 catatcccgg cgttatccgt gaacttgtcg gcgctgcact ttcgcagtaa tcagctgcct
     4741 aatcaggtgt ctgatgcaat gcaagcctgg ggtattgacg gccaccagct gacggtggaa
     4801 atcacggaaa gcatgatgat ggaacacgat accgaaatct ttaagcgcat tcagatcctg
     4861 cgtgatatgg gcgtgggttt atcggtagat gattttggca cgggcttttc cggattatcc
     4921 cgcttagtca gtcttccggt aacggaaatc aaaattgaca aaagctttgt cgatcgttgt
     4981 ctgacggaaa aacgcatcct ggccttactt gaagccatta ccagcattgg gcaaagcctc
     5041 aatttaaccg tcgtggcgga aggggtcgaa accaaagagc aatttgagat gctacgtaag
     5101 atccactgtc gcgttattca gggatatttc ttttcccgcc cccttcccgc cgaagaaatt
     5161 ccaggctgga tgagcagcgt gttaccgctg aaaatctgac aaattcctct cgcccgcact
     5221 cgcgggtttt cctttaaggt gacactgtca cttaaatact gtgatttcag ctacgattcc
     5281 gggagattcc ttccttaact ttccctcttg caccaaaact gcactacgct gcacaaatgt
     5341 tgatcaaaag ttaaaaaata taactttaag ccagcattct ggcgcttatc ccggcatggc
     5401 atgagatctg cataagcgga aagcgcagca atttttgtct tatacaggaa ccgtttatgt
     5461 cggataccac cgaactggtt gatttagccg tgatcttccc tgatctggag atcgaattga
     5521 aatatgcctg cgttgataac atcacaggta aagctattta tcagcaagcg cgttgtctgc
     5581 tacataaaga tgcgattacc gcgctggcga aaagtatcag tatcgcccag ctgtcaggct
     5641 tacaactggt gatttacgat gcgtatcgcc cacaacaagc acaggcgatg ttgtggcaag
     5701 cctgcccaga cccgcaatat gttgttgatg tgacggtcgg ttctaatcac agccgtggca
     5761 cggcgctcga cctgacgctt cgtgatgacg tggaggtggt gaatgatgct aagcgaggaa
     5821 acgtccgccg tacgcccaca aaaacaaacg cgatttaacg gtgcaaaact ggtatggatg
     5881 ctgaaaggca gtccgctcac cgtgaccggc gcagtcatca ttgtattaat gcgactgatg
     5941 atgatttttt caccgtggct ggcgacgcat gatcccaacg ccattgattt aaccgcccgc
     6001 cttttgccgc cttctgcggc gcactggttt ggcaccgatg aagtgggacg cgatctgttt
     6061 agccgcgtac tggtcggcag tcagcaatca attctcgccg gattagtggt ggtcgccatt
     6121 gcgggtatga ttggttcgct actcggatgt ctatccggtg tgcttggcgg acgcgcagac
     6181 gccatcatca tgcgcatcat ggacattatg ctgtcgattc cttcgttggt actgacaatg
     6241 gcactggctg ccgctctcgg gccgagttta tttaacgcca tgctggcgat tgctattgtg
     6301 cgaattccct tttatgtgcg cctggcgcgg gggcaagcat tagttgtacg ccagtatacc
     6361 tatgttcagg cggcgaaaac ctttggcgcg tctcgttggc atctgatcaa ctggcatatt
     6421 ttacgtaact ccctaccgcc gctgatcgtg caggcatcgc tggatatcgg tagcgcgatt
     6481 ttaatggccg ccacgttggg atttattggc ctgggtgctc aacaaccgag tgctgaatgg
     6541 ggcgcgatgg tagcgaatgg tcgcaactat gtactcgatc agtggtggta ttgcgcattt
     6601 ccgggggcag cgattttgct taccgccgtc gggtttaatc tctttggtga tggtattcgc
     6661 gatctgcttg acccgaaagc aggaggaaag cagtcatgac ccaacccgtt ctggacattc
     6721 aacagctgca tttgagtttc cccggtttta acggtgatgt tcacgcgctc aacaatgtgt
     6781 ccttgcagat taaccgcggc gaaattgtcg gtctggtggg agaatccggc tcaggtaaat
     6841 cagtcaccgc aatgctgatt atgcgtctgc taccgacggg cagttattgc gtacatcggg
     6901 ggcagatttc actgctggga gaagatgttc ttaacgctcg ggaaaaacag cttcgtcagt
     6961 ggcgcggcgc acgagtggcg atgatctttc aggaaccgat gaccgccctt aatccgacac
     7021 gtcgaatagg tcttcagatg atggacgtaa tccgccatca tcaaccaata agtcgtcggg
     7081 aagccagagc taaagcgatt gccctgctgg aagagatgca aatcccggat gccgtggaag
     7141 ttatgtcgcg ctatccgttt gagctttcag gtggtatgcg ccagcgggta atgattgcgc
     7201 tggcattctc ctgcgagccg caattgatta ttgccgacga accgacgacg gcgctggacg
     7261 tcacagtaca gttgcaggta ctgcgtttgc ttaaacataa agcacgcgcc agtggaactg
     7321 cggtactgtt catcagccat gatatggccg tggtgtcgca actgtgcgat agcgtttacg
     7381 tgatgtatgc cggaagcgta attgaaagcg gcgggacggc agacgttatc catcatcccc
     7441 ggcatccgta taccattggt ttgctgcaat gcgcaccaga acatggagta ccacgccagc
     7501 cattacccgc cattccaggg acggtaccaa acctcaccca tttgcctgac ggctgcgctt
     7561 ttcgcgatcg ttgctatgcg gcaggtgcac agtgtgaaaa cgtcccggcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcgcctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggtaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttactgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gagctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgagctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat atatatctca
//
LOCUS       DADMGC010000143         9127 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.143, whole genome shotgun sequence.
ACCESSION   DADMGC010000143 DADMGC010000000
VERSION     DADMGC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9127
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.143"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..923
                     /locus_tag="JNZ73_003494"
     CDS             <1..923
                     /locus_tag="JNZ73_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8733635.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="JNZ73_003495"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="JNZ73_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HAY8733636.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="JNZ73_003496"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="JNZ73_003496"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HAY8733637.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCKILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="JNZ73_003497"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="JNZ73_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HAY8733638.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="JNZ73_003498"
     CDS             4446..4805
                     /locus_tag="JNZ73_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HAY8733639.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="JNZ73_003499"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="JNZ73_003499"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HAY8733640.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="JNZ73_003500"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="JNZ73_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HAY8733641.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALDHNCTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="JNZ73_003501"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="JNZ73_003501"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY8733642.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2085 a         2210 c         2461 g         2371 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgccg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ctacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccacgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg ataggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat attttacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaatcaac ataatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcac gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggct
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tttgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gttgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggatcataac tgtactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcacc
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gcgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggct tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
     9061 aaacacgccg ccgggtcagc ggggttctcc tgagaattcc ggcagagaaa gcaaaaataa
     9121 atgcttg
//
LOCUS       DADMGC010000144         9039 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.144, whole genome shotgun sequence.
ACCESSION   DADMGC010000144 DADMGC010000000
VERSION     DADMGC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9039
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.144"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="JNZ73_003502"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="JNZ73_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HAY8733643.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1860)
                     /gene="phoA"
                     /locus_tag="JNZ73_003503"
                     /pseudo
     CDS             complement(446..1860)
                     /gene="phoA"
                     /locus_tag="JNZ73_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
     gene            complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="JNZ73_003504"
     CDS             complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="JNZ73_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HAY8733644.1"
                     /translation="MKNLITELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(2403..2606)
                     /locus_tag="JNZ73_003505"
     CDS             complement(2403..2606)
                     /locus_tag="JNZ73_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733645.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2684..3778
                     /gene="ddlA"
                     /locus_tag="JNZ73_003506"
     CDS             2684..3778
                     /gene="ddlA"
                     /locus_tag="JNZ73_003506"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAY8733646.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASATCMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGELVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3802..4014)
                     /locus_tag="JNZ73_003507"
     CDS             complement(3802..4014)
                     /locus_tag="JNZ73_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 family protein"
                     /protein_id="HAY8733647.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4274..4582
                     /locus_tag="JNZ73_003508"
     CDS             4274..4582
                     /locus_tag="JNZ73_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HAY8733648.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="JNZ73_003509"
     CDS             complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="JNZ73_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HAY8733649.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGSYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="JNZ73_003510"
     CDS             complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="JNZ73_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HAY8733650.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7320..8477
                     /gene="ampH"
                     /locus_tag="JNZ73_003511"
     CDS             7320..8477
                     /gene="ampH"
                     /locus_tag="JNZ73_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HAY8733651.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8478..8981)
                     /locus_tag="JNZ73_003512"
     CDS             complement(8478..8981)
                     /locus_tag="JNZ73_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8733652.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
BASE COUNT         2240 a         2314 c         2149 g         2336 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagattgt tgcgttgctt gattattaca ttcgcgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cggttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca aggattcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca ccggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg
     1561 taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggcagt
     1621 aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc
     1681 gctaagagaa tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg
     1741 tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc
     1801 ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac
     1861 tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg
     1921 ccgtgacaac tttatgacag ctattgaaaa gattaacttt ttactgacga ggatgcttca
     1981 ataacttctt tacgtaatcg cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcgg
     2041 gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg tcattttgcg
     2101 ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc gcacacagtt
     2161 ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagtaatg agatttttca
     2221 tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag
     2281 tgtctggaag tattattttt atgacaaagg aaatactttg cgcaaatatt ttttgtttga
     2341 ttaccagcga aacgtttctc ttatttaaat aattacccac acaaaatata ggcttttaat
     2401 tatcaacctt tattataaat aaaagctatg aaatatattc tcttcgttaa cttataatta
     2461 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
     2521 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
     2581 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
     2641 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
     2701 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
     2761 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
     2821 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
     2881 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
     2941 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt
     3001 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
     3061 gtttgtaggt tctgatgttc tggcttcagc aacctgtatg gataaagatg tcaccaaacg
     3121 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
     3181 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
     3241 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
     3301 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
     3361 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
     3421 gctcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     3481 gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc
     3541 cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     3601 cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     3661 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt
     3721 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
     3781 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     3841 aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     3901 ccaacctttt gcttcaaatc ccagtacccc caccacgcca ttaagaatga atataaggcc
     3961 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
     4021 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     4081 cattttcgta attgttaata aatttaactt aaggttgcca ttttgtgact cacaacgaag
     4141 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     4201 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     4261 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     4321 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     4381 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     4441 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     4501 cgggctgatc ccgtttttag caggctgcct catttttgca gtggtggcgc tatggctgca
     4561 ctggcgacat cgccgccagt aaaatttacg ttgccagatg cggcctgaaa cgccttatct
     4621 ggcctgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc
     4681 ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca
     4741 ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc
     4801 ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt
     4861 tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg
     4921 ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc
     4981 ttactgactg cgttctgaaa cgcagcatta cggctggcgt accagccagc gttaaaatca
     5041 gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagaacca
     5101 aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac
     5161 ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg
     5221 cgcaccggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg
     5281 tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt
     5341 ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc
     5401 cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga
     5461 tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc
     5521 gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg
     5581 tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg
     5641 ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac
     5701 aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta
     5761 tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg
     5821 tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg
     5881 cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca
     5941 acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg
     6001 cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg
     6061 ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc
     6121 agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg
     6181 atccctacca ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc
     6241 agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc
     6301 agcaccggca ggaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc
     6361 tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata
     6421 tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac
     6481 acgtagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc
     6541 cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt
     6601 agcgcggttt gaatcagatc atagaacggc gcataccagg cgttgacggc gaccccgact
     6661 tccaccaaaa accaagtgac gaagataatc agcgcagtac cgagtattga ccagtattgc
     6721 caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag
     6781 taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc
     6841 ccggaagcgc cggtgatacg tgccacccag tcacccccac cggcttgcca gaagataacg
     6901 gcaatcaatg cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac
     6961 ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc
     7021 atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct
     7081 tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt
     7141 acataagcgg attaacacgc gcaggatcac aaatgcaggc agtggtgagc aggaaaaaga
     7201 tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat
     7261 ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt
     7321 tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg
     7381 cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc
     7441 atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc
     7501 gcgtctttcg tagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt
     7561 cggtcgtgcg tattgcttcc ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc
     7621 tcgaccaggg gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac
     7681 gcgtgccgac ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg
     7741 ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc
     7801 gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg
     7861 cggcgtactc taaccttgcg ttcgacctac tggcagatgc actggcgaat gcgtccggca
     7921 agccttatac ccagttgttt gaagagcaga ttacccatcc attggggatg aaagacacca
     7981 cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc
     8041 cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata
     8101 tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag
     8161 atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg
     8221 tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc
     8281 gtccgaggat tattcagaag acaggcggtg gtggcggttt cattacctat atggcgatga
     8341 tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta
     8401 aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg
     8461 ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct
     8521 ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa
     8581 cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct
     8641 tcattccagt caatcattga taataatgtt gcgcgggttt gtttggctgg tagatgatat
     8701 cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca
     8761 gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc
     8821 agagagataa ctccttctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat
     8881 ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg
     8941 agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta
     9001 atgcccgtca aatccttgac gggcattatt tagattaaa
//
LOCUS       DADMGC010000145         9010 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.145, whole genome shotgun sequence.
ACCESSION   DADMGC010000145 DADMGC010000000
VERSION     DADMGC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.145"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(95..1471)
                     /gene="trkA"
                     /locus_tag="JNZ73_003513"
     CDS             complement(95..1471)
                     /gene="trkA"
                     /locus_tag="JNZ73_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HAY8733653.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1493..2782)
                     /gene="rsmB"
                     /locus_tag="JNZ73_003514"
     CDS             complement(1493..2782)
                     /gene="rsmB"
                     /locus_tag="JNZ73_003514"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAY8733654.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2828..3775)
                     /gene="fmt"
                     /locus_tag="JNZ73_003515"
     CDS             complement(2828..3775)
                     /gene="fmt"
                     /locus_tag="JNZ73_003515"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAY8733655.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3790..4299)
                     /gene="def"
                     /locus_tag="JNZ73_003516"
     CDS             complement(3790..4299)
                     /gene="def"
                     /locus_tag="JNZ73_003516"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAY8733656.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4447..5571
                     /gene="dprA"
                     /locus_tag="JNZ73_003517"
     CDS             4447..5571
                     /gene="dprA"
                     /locus_tag="JNZ73_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAY8733657.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNIIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5543..6016
                     /locus_tag="JNZ73_003518"
     CDS             5543..6016
                     /locus_tag="JNZ73_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HAY8733658.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6045..6587
                     /locus_tag="JNZ73_003519"
     CDS             6045..6587
                     /locus_tag="JNZ73_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAY8733659.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6592..7164
                     /gene="tsaC"
                     /locus_tag="JNZ73_003520"
     CDS             6592..7164
                     /gene="tsaC"
                     /locus_tag="JNZ73_003520"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HAY8733660.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7169..7987
                     /gene="aroE"
                     /locus_tag="JNZ73_003521"
     CDS             7169..7987
                     /gene="aroE"
                     /locus_tag="JNZ73_003521"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAY8733661.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7984..8241
                     /locus_tag="JNZ73_003522"
     CDS             7984..8241
                     /locus_tag="JNZ73_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HAY8733662.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8217..8771)
                     /locus_tag="JNZ73_003523"
     CDS             complement(8217..8771)
                     /locus_tag="JNZ73_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAY8733663.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2255 a         2189 c         2248 g         2318 t
ORIGIN      
        1 aacaaatgtt aagccatttt cctgccagga atgaatttaa ttaatccttg ataatcaaag
       61 ggaaataata aggcgtcatt agacgcctta ttaattacaa gaagaaaggg cttggctgga
      121 agagtctttc gacgtcggta ataaactttt tatctgtgag gaacataatt acgtgatcgc
      181 cttgctcaat gcgcagattg tcattggcaa tcatcacgtc gtttccacgt accaccgctc
      241 caataatcgt tcctggcggt agcttgattt cgtcaatgac tctgccgaca acgcgtgagg
      301 tgctttcatc accgtgagca acggcttcaa tagcttctgc tacgccgcgg cgcaatgagg
      361 aaacaccaac aatatctgct tttcgcacat ggctaagcaa cgcagaaata gttgcttgtt
      421 gtggtgaaat tgcaatatcg atcacgctcc cctgaaccag atccacataa gcgcgacgct
      481 ggatcaatac catcaccttt ttcgcaccca tacgtttggc aagcatggcg gacatgatat
      541 tggcctcgtc atcgttggtg acggcaataa acagatcaac ttgatcgata tgttcttcgg
      601 ccagcagttc ttgatccgac gcatcaccaa aaaagacgat cgtattctgc aacttttccg
      661 ccagttcggc agcgcgctgc tgattacgtt cgatgagttt aacgctgtaa tctttttcca
      721 gacgacgcgc cagccctgca ccgatattac cgccaccaac cagcatgatc cgtttatacg
      781 gtttttccag tcgctgtaat tcactcatca ccgcgcggat atgctgtgaa gcggcaataa
      841 agaacacttc atcaccagct tcaacaatgg tcgaaccttg tggacgaatg gggcgatcgt
      901 ggcggaaaat tgctgccaca cgagtatcga tatgtggcat atgttcgcgc atggtcgaaa
      961 gtgcattacc aatcagcggg ccgccataat aggctttaac tacagcgagg ctgactttac
     1021 cctcagcgaa gttcaccacc tgcaatgcgc ctggatactc aatcagtcgg taaatattat
     1081 cgataaccaa ctgctctggt gcgatcagat gatcaatcgg tacagcatct gaatgaaata
     1141 gcttatcggc atcgcgtacg tagtctggtg agcggatgcg agcgatgcga ttaggggtgt
     1201 tgaaaagtga gtaggctacc tggcaggcaa ccatattggt ttcatctgaa ctggttacag
     1261 caaccagcat atcggcgtcg tcggcacctg cctcccgcaa tacgcgtgga tgagagccat
     1321 gcccctgcac gactcgcagg tcaaatttat cctgcaaggt ccgcagacgc tcaccgttgg
     1381 tatcgacaac agtaatatcg ttgttctcgc caaccaggtt ttccgccagt gtgccgccaa
     1441 cctggccggc acccagaatg ataattttca tcagtcgcga cccgttatct catcactttt
     1501 tgattagctt agcgtaaaag aagccgtcgc cctcttcggc accaggctga ttttgtttac
     1561 ccggttgctc tggtgttcct gtttcgcaaa gttcggcatc agcggtacgt tgcaaaaagg
     1621 ctttaatctg caggctattc tcttccggta acaccgaaca ggtggcatag accagggttc
     1681 caccggattt taaatgcggc caaatggcgt cgagaatttc agactgcaat tgcgcgagtt
     1741 ccgggatatc gcgatcgcga cgtaaccatt taatatctgg atggcgacga atcacccccg
     1801 ttgctgaaca aggcgcatct aataaaatgc gatcaaactg ttgctcgcca caccattggg
     1861 aagggtaacg gccatcacct tgtttcacgg ttgccttcat accaaggcgt tttaaattgt
     1921 cgtaaacgcg agagaggcgc tgttcgtcaa tatcaaccgc aacaacctgc gcttctggtg
     1981 ccacctcaag gatatgcgtt gttttaccgc cgggggcggc acaaagatcc aagatgtgtt
     2041 caccgttttg tggcgcaagc caggtcatgc aaccttgtgc tgatgcatcc tgaacggtaa
     2101 cccatccgtc ttcaaaacca ggtagcgcat gaacaggtgc aggtgtttcc agccgtacag
     2161 catcagggta atccgcatgc gggaaacctt tcattcctgc ttcatccagc aatgcaagcc
     2221 agctgtcgcg ggaatgatgc gtacgattaa tacgcagcca cattggcgga cgctggttat
     2281 tggcttcggc gatggattgc cactgctctg gatacgcttt ttgcagacgc ttcagcaacc
     2341 aggaaggatg cagataacgt gcatcacggg tattaaactc ggctaataac tcttcttgcc
     2401 gacgctggaa ctggcgtaat acaccgttaa tcaacccttt aagttgcgga cgcttaattg
     2461 cgatagcgcc ttcaaccgtt tcagccagcg cagcatgagg tggaatgcgg gtataaagca
     2521 gttgatacaa accaaccata atcaggtaat gcacagtgcg ctgtttgccg gtcatcggac
     2581 gggccattaa cttattaatc agccagtcta actgcgaaag cgtacgcagt acgccaaagc
     2641 acaactcttg aagaagtgct ttgtctttat cggaaacttt ttgctggagc ggtggcagaa
     2701 tgttgcttaa tgattgccct tgctcgacga cttgttcaac ggcctgggcc gccatgctac
     2761 gtaaattacg ttgttttttc ataagtataa aaatgctcgg caagaccggg cttagaagag
     2821 tggactatca gaccagacgg ttgcccggaa caaaccattc ccgacgagag ttcaggaggt
     2881 cttgcgcgct catcgctttc ttacccgcag gttgtaacga gagcaggttc aggatgccat
     2941 caccagtcgc aacctgaatg ccttgtttgt tggcttcaag gatcgttcct ggtgcagcgt
     3001 tggttgccgt atcaatgacc gacgctttcc agactttaac cggctgtcct tcaatttcca
     3061 gccagctcat tggccatggg ttgaaagcgc gaatgcagcg ttcaagctgt gctgccgaaa
     3121 gtgaccagtc aatacgcgct tcttctttac tcaacttctc ggcgtaagtg acaagagttt
     3181 cgtcctgaac ttctggtttc gccgtgccgt ctgccaattg tttcaacgtg gtgataagcc
     3241 cttgtgggcc aagctctgcc agcttgtcgt acagcgtacc actggtatct tctgcagtaa
     3301 tcgggcagga gagcttatag agcatgtcac cggtgtctaa accaacatcc atttgcataa
     3361 tggttacacc agtttctgca tcacccgccc atagtgagcg ttggattggt gcagcaccgc
     3421 gccagcgtgg cagcagtgaa ccatgaacgt tgatacagcc aagacgcggc atctccagca
     3481 cagctttagg cagaattaaa ccataggcga cgacgaccat aacatcagcc tgcaaatcgg
     3541 cgaccagttg ctggttttct tgtggacgca gggaaacagg ttgaaaaacg ggcagacctt
     3601 cttcctcagc cagaacttta atcgggctgg gcatcagttt tttaccgcgt cctgccggtc
     3661 ggtctggctg ggtgaacacg ccaacgacgt tatgaccaga agacaacagc gcgtcgagat
     3721 gacgcgctgc aaagtcaggt gtacccgcaa aaataatacg tagtgattct gacacgttag
     3781 ttcttatcct taagcccggg ctttcagacg atccagtttt tcaactttct gacgaattcg
     3841 ttgttgtttc agcggtgaca gataatccat aaacagtttg ccaaccaggt gatccatctc
     3901 atgctgaata cagatagcta acagaccctc tgcttccagt tcaaatggtt taccgtcgcg
     3961 gtcaagggcg cgaattttaa ctttctctgc gcgcggcact aaagcacgtt gttcagggat
     4021 cgacaggcaa ccttcttcaa tgcctgtttc gccgcttttt tctaaaagct ccggattgat
     4081 taacaccagc cgttcgtcac ggttttccga aacatcaata acaatgatac gttgatggat
     4141 atcaacctgg gttgccgcca ggccaatacc ttcttctgcg tacatcgtct cgaacatatc
     4201 atcgacgata cgctgaattt ctgcattcac ttcttctacc ggtttagcaa ctttgcgaag
     4261 ccgctcgtcc ggaatatgta acacttgcaa aactgacata aatctccaga gatgtgttca
     4321 ggagttagaa agattatttc ttctattcta gacaaatccc cctctgattg acagcatcac
     4381 ttgattgaca gcatcactga ccaatcgcaa agattgctaa ggcttgctta tgacagggag
     4441 ataaggatgg tcgatacaga aatttggctg cgtttaatga gcatcagcag cttgtacggc
     4501 gatgatatgg tccgtatcgc tcactggctg gcaaaacagt cgcatattga tgcggttgta
     4561 ttgcagcaaa cagggcttac attgcggcag gcacaacgct ttctttcatt tccacgaaag
     4621 agtatcgaaa gctcactttg ttggttggag caacccaacc atcatttaat ccctgcggac
     4681 agcgaatttt atcctcctca acttcaggcg acgacagatt accccggcgc actgtttgtt
     4741 gaaggagaac tgcacgcgct gcattcattt cagcttgccg tagtggggag tcgggcgcat
     4801 tcatggtatg gcgagcgatg ggggcgatta ttttgcgaaa ctctggcgac gtgtggagtg
     4861 acaattacga gtggactggc gcgtggaatc gatggtgtgg tgcataaagc ggccttacag
     4921 gtaaatggcg tcagcattgc tgtattgggg aatggactta atatcattca tccgcgtcgc
     4981 catgcccgac tggctgccag tctgtttgaa cagggtggcg ctctcgtctc ggaatttccc
     5041 ctcgatgttc caccccttgc ttacaatttc ccacgaagaa atcgcattat cagtggtcta
     5101 agtaaaggtg tactggtggt ggaagcggct ttgcgcagtg gttcgctggt gacagcacgt
     5161 tgtgcgcttg agcaggggcg tgaagttttt gccttgccag gtccaatagg gaatccggga
     5221 agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga accggaagaa
     5281 attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga aaattcattt
     5341 tattcaccag atcagcaaga cgtggcattg ccatttcctg agctcctggc taacgtagga
     5401 gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt gccagaggta
     5461 gttactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc cggcggctat
     5521 gtccgattga ggagggcatg ccatgttcga cgtactaatg tatttgtttg aaacctatat
     5581 tcacactgaa gctgagttgc gtgtggatca agacaaactt gaacaggatc ttaccgacgc
     5641 aggttttgag cgagaagata tctacaatgc cctgctatgg ctggaaaaac ttgctgatta
     5701 tcaggaaggg ttggcagaac cgatgcaact ggcctcggat cctctctcca tgcgtattta
     5761 tacaccggaa gagtgtgaac gactggatgc cagctgccgt gggtttctgc ttttccttga
     5821 gcagattcag gtgctcaacc ttgaaactcg tgaaatggtg atagagcgag tgctcgcgct
     5881 ggataacgca gagttcgaac tggatgatct gaaatgggtg atcctgatgg tgttgttcaa
     5941 catcccgggc tgcgaaaatg cgtaccagca aatggaagaa ttactctttg aagtgaatga
     6001 aggtatgctg cattaatcat ttctttaatt cagcataagt tgttatggca aaatcagcac
     6061 tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg gttattcgat
     6121 ccgggaaaca cggtccgttt cttggatgct cacagtatcc ggcgtgtgac tacgtccgtc
     6181 ctctgaaatc ttcagcggat ggacatattg tcaaagttct ggaggggcag gtttgccccg
     6241 tatgtggcgc aaatctggta ttacgccagg gacgctttgg tatgtttatt ggttgcagta
     6301 actaccctga atgcgaacat accgaactta tcgataaacc ggacgaaaca gcaattacat
     6361 gcccccaatg tcggacgggc catctggtcc agcgccgctc ccgttatggc aaaacatttc
     6421 actcctgtga tcgctacccg gagtgtcaat ttgccattaa cttcaaacca atagctggag
     6481 aatgccctga gtgtcattat ccgctactca tcgaaaagaa aaccgcgcag ggtgtaaaac
     6541 acttttgtgc cagtaaacaa tgtggaaagc cggtttcggc ggaataataa cgtgaataat
     6601 aacctgcaag gagacgctat cgcagctgcg atagatgtcc tcaatgaaaa acgtgtcatc
     6661 gcctatccaa cggaagccgt tttcggtgtc gggtgcgatc ctgatagcga aacagcagtg
     6721 atgagactgt tggagttaaa acagcgtccg gttgataagg ggctgatttt aatcgcggca
     6781 aattacgagc agcttaaacc ctatattgat gacaccatgt tgactgacgt gcagcgtgaa
     6841 accatttttt cctgctggcc aggtcctgtc acctttgtct ttcccgcgcc tgcgacaaca
     6901 ccgcgctggt tgacgggccg ctttgattcg cttgctgtac gagtcaccga ccatccgttg
     6961 gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc caacttgagt
     7021 ggattgccac cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc ggcgttcccg
     7081 gttgtgcctg gtgaaacggg ggggcgttta aatccttcag aaatccgcga tgccctgacg
     7141 ggtgaactgt ttcgacaggg gtaacataat ggaaacctat gctgtttttg gtaatccgat
     7201 agcccacagc aaatcgccat tcattcatca gcaatttgct cagcaactga atattgaaca
     7261 tccctatggg cgcgtgttgg cacccatcaa tgatttcatc aacacactga acgctttctt
     7321 tcgtgctggt ggtaaaggtg cgaatgtgac ggtgcctttt aaagaagagg cttttgccag
     7381 agcggatgag cttactgaac gggcagcgtt ggctggtgca gttaataccc tcaagcggtt
     7441 agaagatgga cgcctgctgg gtgacaatac cgatggtgta ggcttgttaa gcgatctgga
     7501 acgtctgtct tttatccgcc ctggtttacg tattctgctt atcggcgctg gtggagcatc
     7561 tcgcggcgta ctactgccac ttctttccct ggactgtgcg gtgacaatca ctaatcggac
     7621 ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac actggcagta ttcaggcgtt
     7681 gggtatggac gaactggaag gtcatgagtt tgatctcatt attaatgcaa catccagtgg
     7741 catcagtggt gatattccgg cgatcccgtc atcgctcatt catccaggca tttattgcta
     7801 tgacatgttc tatcagaaag gaaaaactcc ttttctggca tggtgtgagc agcgaggctc
     7861 aaagcgtaat gctgatggtt taggaatgct ggtggcacag gcggctcatg cctttcttct
     7921 ctggcacggt gttctgcctg acgtagaacc agttataaag caattgcagg aggaattgtc
     7981 cgcgtgaatc aggccatcca gtttccggac agggaagagt gggacgagaa taaaaaatgt
     8041 gtatgttttc ccgctctcgt gaatggtatg caactgacat gcgcgatctc tggcgagagt
     8101 ctggcgtatc gctttactgg agatacgcca gaacagtggt tagcgagttt tcgtcagcat
     8161 cgctgggacc tggaagaaga agcggaaaac ttaattcagg aacaaagtga agatgatcaa
     8221 ggctgggtct ggttaccctg atccagatat tcgtccttcc atttcacgta attattcgcg
     8281 gaatagcgta acccagcctt ctcttcatca cttaacgggc ggatctgttt gacggggcta
     8341 ccgagataca gatatccgct ctccagccgt ttattttgtg ggaccagact acccgcacca
     8401 atcatcacat catcttctac tattgcgcca tcaagtaaaa ttgagcccat cccaaccaaa
     8461 actcgattgc caatggtgca gccgtggagc atcaccttgt gaccaacagt gacatcttcg
     8521 ccaatggtta atgggttgcc atctgggttg tacgaggatt tatgagtgac atgcaacata
     8581 ctgccatcct ggatattggt gcgtgctccg atctgtacat aatgtacatc tccacgaatc
     8641 acaacgagcg gccagatccc cacatcatca gccagacgaa cgtcaccaat cacgacactg
     8701 ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta tgggcgtaaa
     8761 acatcagaca tacttacctc agcaataaat gatttactaa tgactttggg ggcattattg
     8821 gccttgtgca agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgat tgcagcaaaa
     8881 agggtgaaaa aacaacaaac agaaaaaaag atcaaaaaaa tacttgtgca aaatattggg
     8941 atccctataa tgcgcctccg ttgagacgac aacgtgaaac acttcacagg atggtcggaa
     9001 caacgaagag
//
LOCUS       DADMGC010000146         9000 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.146, whole genome shotgun sequence.
ACCESSION   DADMGC010000146 DADMGC010000000
VERSION     DADMGC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.146"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            101..2987
                     /locus_tag="JNZ73_003524"
                     /pseudo
     CDS             101..2987
                     /locus_tag="JNZ73_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(3097..3945)
                     /gene="alkA"
                     /locus_tag="JNZ73_003525"
     CDS             complement(3097..3945)
                     /gene="alkA"
                     /locus_tag="JNZ73_003525"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HAY8733664.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            4079..5431
                     /gene="yegD"
                     /locus_tag="JNZ73_003526"
     CDS             4079..5431
                     /gene="yegD"
                     /locus_tag="JNZ73_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8733665.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(5443..5664)
                     /locus_tag="JNZ73_003527"
     CDS             complement(5443..5664)
                     /locus_tag="JNZ73_003527"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8733666.1"
                     /translation="MFRPNEAVTPADRQAVMAKMAAKRHRLESTLTVGATELQRFRLH
                     APARTMPLMEPLRQAAEKLAQAQADLSRC"
     gene            complement(5860..7383)
                     /locus_tag="JNZ73_003528"
     CDS             complement(5860..7383)
                     /locus_tag="JNZ73_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HAY8733667.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHGNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCLWCTLDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKLALAALHDTARERQK"
     gene            complement(7380..8141)
                     /locus_tag="JNZ73_003529"
     CDS             complement(7380..8141)
                     /locus_tag="JNZ73_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HAY8733668.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8138..8797)
                     /locus_tag="JNZ73_003530"
     CDS             complement(8138..8797)
                     /locus_tag="JNZ73_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY8733669.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2195 a         2386 c         2354 g         2065 t
ORIGIN      
        1 gtgacatcac cccccggatc atggacgact gggggcagta atcatgccgg gtctggctgg
       61 ctaacacccc attataaggg gtacgaataa agtaacgggg ctggttgtcc tgctgacgcc
      121 cggagacgat cctctcaggg catttttgat atgggtactg tcagaatcca tcggcgctct
      181 ggcactggtg ccgctgggat tgttatttaa accacactat ctgctgcgcc atcgcaaccc
      241 acggttgctt tttgagtcgc tgctcacgtt agccatcaca ctgacgttaa gctggctttc
      301 gatgctgtat ctgccgtggc cttttacttt cattattgtg ctgttgatgt ggagcgccgt
      361 gcgcctgcca cgaatggaag cctttttgat cttccttacc acggtgatga tggtgtcgct
      421 gatgatggcc gcggatccct ccctgcttgc tacgccgcgt acgtacctga tgagccatat
      481 gccgtggcta ccgtttttgc tgatcctgct gcccgccaac atcatgacca tggtgatgta
      541 tgcctttcgt gcggaacgca aacacatttc cgaaagcgaa acccgctttc ggaacgcgat
      601 ggaatattcc gctatcggta tggcgttagt gggcaccgag ggacaatggc tgcaatccaa
      661 caaagcactc tgccagtttc tcggttacag tcaggaagag ctgtgcggac tcacctttca
      721 gcaactgacc tggccggagg atctcaataa agatctccaa caggttgaaa agctgataag
      781 cggtgaaata aacacctatt caatggaaaa acgctactac aaccgcaatg gcgatgttgt
      841 ctgggcgttg cttgccgtct cactggtgcg ccacacggat ggcacgccgc tctattttat
      901 cgctcagatt gaagacatta acgagctaaa acgcaccgaa caggtgaatc agcaactgat
      961 ggagcgcatc acgctggcta acgaagcggg cgggattggc atctgggagt gggagctgaa
     1021 gccgaatatt tttagctggg ataagcggat gttcgagctg tatgaaattc ctccgcatat
     1081 caaaccgaac tggcaggtgt ggtacgagtg cgtgctgccg gaagatcgcc agcatgccga
     1141 aaaagtgatt cgttgcaatc acgctcgccc tttaagctgg aatttcgcat tgccgtgaaa
     1201 gatggcatcc gccatatccg cgccctcgcc aaccgggtac tgaataaaga aggcgaagtc
     1261 gaacgcctgc tcggcattaa tatggatatg accgaggtta aacagcttaa cgaggcattg
     1321 tttcaggaaa aagagcgcct gcacattacg ctggattcca tcggtgaagc cgtggtctgt
     1381 attgatatgg cgatgaaaat tacctttatg aatccagcgg cggagaagat gagcggctgg
     1441 acgcaggaag aagcattagg tgttccgctc ctgacggtgt tgcatattac ttttggcgac
     1501 aacggaccat taatggagaa catttacagt gccgacacct cacgttccgc gattgaacaa
     1561 gatgtggtgt tgcactgccg gaacggcggc agttacgacg tgcattacag tattacgccg
     1621 ttaagtactc tggacggcag caatataggc tctgtactgg tgattcagga cgtcaccgaa
     1681 tcacgcaaaa tgctgcgcca gctgagctac agcgcctccc atgatgcact gacgcatctc
     1741 gccaatcgcg ccaattttga gaagcaactg cgtatcctgc tgcaaacggt aaacagtaca
     1801 catcagcgac atgccctggt gtttatcgat cttgatcgct ttaaagcggt gaatgacagc
     1861 gccgggcatg cggcgggtga cgctttactg cgcgaactgg cgtcgttgat gctgagtatg
     1921 ctgcgctcca gcgacgtgct ggcgcgactc ggtggtgatg aatttggtct actgttgcca
     1981 gactgtaata tcgaaagtgc gcgttttatc gctacacgca ttatcagtgc cgtgaatgat
     2041 tatcacttta tatgggaagg ccgtgtgcat cgggtaggtg ccagtgccgg gattaccttg
     2101 attgatgaca acaatcatca ggcggcagaa gtgatgtcgc aggctgatat cgcctgttat
     2161 gcctccaaaa atggtggacg gggccgggtg acggttacga accacagcaa gctgccgcac
     2221 atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg gatgattaaa gagaatcagt
     2281 tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc ggaagcgcgt aatttgtggc
     2341 tgatttcact taagctctgg agttgcgaag gcgagattat tgatgaacaa aaatttcgtc
     2401 gtagcttcag cgatccggca cttagccatg ctcttgaccg acgggtattc cacgaatttt
     2461 tccagcaggc cgcaaaagcg gttgccagta aaggcataag catcgccctc cccctttccg
     2521 ttgccggttt gagtagcgcc acgctggtga atgatctgct tgagcagctg gaaaatagcc
     2581 ctctaccacc acggttatta catctgatta ttccggctga agcgatttta gatcacgcag
     2641 aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt gctcagccag gtgggccgcg
     2701 atctgcaaat cttcaactcg ctgaaagcga atatggcaga ttacctgcta cttgatggtg
     2761 agttatgcgc caacgtgcag ggtaatttga tggatgagat gctgattacg attattcagg
     2821 ggcacgctca gcgactcggg atgaaaacca tcgccgggcc agtcgtttta cccttagtga
     2881 tggatacgct ttctggcatc ggcgtcgatc tgatttatgg tgatgtgatt gccgatgccc
     2941 aaccgctgga tttgctggtg aatagcagtt atttcgcgat taactgagag agcgtttgcc
     3001 cattgcctga tgcgatgcta acgcgtctta tcaggcctac aaatcgctca ttccgcaggc
     3061 gggataaggc gctcgcaccg cttccggcga ccaacgtcat gcttcgtctg gttgccagcc
     3121 ttccgtatac cagatatgca acagcgcata agaacgccag ggcttccagc gctcggcata
     3181 acggcggatt tgtgccggtg tcatccccag aaatcgctgt ttaatcagat aatcatccgg
     3241 cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa taattcgccg tccagcgccc
     3301 gatacccgga aaagtttgca gcgttttcat cgcctgctcc acatcgcccg gtattgtcat
     3361 tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc agcgcctctg cccgtttcaa
     3421 cggcatacct aacgctttta atgcctgcgg gtcggctgct gccagccgct gaggcgtggg
     3481 gaagcagata tactccggaa aatcatccag ccgttcgcca taaagctgtg ccactctggc
     3541 ggtcaatttt gccgccatcg ccacgctcac cagttggcct aaaatcgccc gcacgccctg
     3601 ctcaaaagca tcaacactgc cgggtaaacg caatccgggc cgcgccgcgc ctaacttgcc
     3661 caacgcaccg ttaacaatct gcgggttaca ttgcagatca agcaggcggc tcattttcgc
     3721 cagacactca gcggcaacgg gttctaaacc tgcacttaaa tttatgtgca gagtatggcg
     3781 ggctatatcc ggaatagcag tcaccacgcc gcgatattcg cccactgcca gactacgggc
     3841 ataataactg tccgcgaccg tttccacacc actcaccgca cgggcggcga gaaatcccaa
     3901 catccacgac cagtcatacg gcggctgcca gttcaggata tacatcgcat ctccttattc
     3961 atccgctttc agcataaacg ttattcagac gttacgcttt gctttcatat tccggttgtc
     4021 gcgacggcaa catttcgcta aagtcacgcc ccttcttcac cggcatgggg attatttcgt
     4081 gtttattggt tttgattacg gtacagcaaa ctgttcagtg gcggtcatgc gtgacggtaa
     4141 accgcatttg ctaaaaatgg aaaacgacag cacgctgctg ccttcaatgc tttgcgcgcc
     4201 aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat gacgttccgg cagacgacga
     4261 tgaaacgcag gcgttgttac gacgggcgat tcgttataac cgcgaagaag atatcgatgt
     4321 tacagcgaaa agcgtgcagt tcggtctttc ctcactggca cagtacattg atgatccaga
     4381 agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt gccagcggct taaaaccgca
     4441 gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg ttgcacattc gccagcaggc
     4501 gcaggcgcag ctgccagaag caattactca ggcggtgatt ggtcgcccga tcaacttcca
     4561 ggggctgggc ggcgatgaag caaacgccca ggcgcagggg attctggaac gcgcggcgaa
     4621 gcgtgccggg tttaaggatg tggtattcca gtacgagccg gtagcggctg ggctggatta
     4681 cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg gatatcggcg gtggtacgac
     4741 tgactgttca ttgctgctga tggggccgca gtggcgttcg cgtctcgatc gtgaagccag
     4801 tctgctgggt cacagtggtt gtcgtattgg cggtaacgat ctggatatcg ccctagcgtt
     4861 taaaaacctg atgccactgc tgggtatggg tggcgaaacc gaaaaaggca tcgccctgcc
     4921 gatcctgccg tggtggaatg cggttgccat caacgacgtt cctgcacaga gtgatttcta
     4981 cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc gatgcccgcg aaccggaaaa
     5041 agtggccctg ttacagaaag tctggcgtca gcgtttaagc tatcgcctgg tgcgtagcgc
     5101 agaagagtgc aaaattgctc tttcaagcgt agcggaaacc cgcgcctcac tgccgtttat
     5161 cagcaatgaa ctggctacgc tgattagcca gcgagggctg gaaagcgccc tcagccagcc
     5221 actggcgcgg attctggaac aggtgcaact ggccctggat aacgcccagg aaaaaccgga
     5281 cgttatctac ctgaccggcg gtagcgcccg ttctccgctg attaaaaaag cgctggcaga
     5341 acagttgccg ggcattccga ttgcaggcgg cgatgacttt ggctccgtca ccgccgggct
     5401 ggcacgctgg gcggaagtgg tatttcgtta aaagaaaatg gctcagcagc ggcttaaatc
     5461 agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac ggttccatca acggcatggt
     5521 ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc gcgccgacag tcaacgtcga
     5581 ttccagccga tgtcgtttgg cggccatttt cgccattacc gcctgtctgt ctgctggcgt
     5641 taccgcttcg ttcggcctga acacaaaacg gcgttcacag ctcgctttcc agtcgatgac
     5701 cgcctgggtc agatgatcac caaacccttt aacttgctta acgccacgac gggtaacacc
     5761 tgctgctgtt tcaataccaa aagaccataa cgccgcttta cgcgcagggc caacgccggg
     5821 aatagaggca acatcaataa aaaatccctc cagaaacttc tacttctgcc gttcccttgc
     5881 agtgtcatga agtgctgcca gagcgagttt ctcttcttcc ggtaacccga gaatttcgtc
     5941 cttcattttt tcgagcatcg tccgtttggc gataaaacct tccaggccgc ctacctgctg
     6001 gatctggcgg actaaatggt catagtccat tttcgcacga ttaaatgcct cacggcgttg
     6061 ctgaacttct gctttgtacg ctttgcttgt cagactgcca ataatccaga tagccgccag
     6121 cacaggaacc aaaataatat aacgcggttc tgcctgaagg ccgcaaagca gcagcgataa
     6181 tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc cgtaacaggc ctaaaggaag
     6241 cggcctgcca gtcggttgga aatgatcggg taatggcagt tgcaatgctg gcggtgctac
     6301 tgacgccatc accatcgccc agacttttgc cagcacaaaa tccccgccgg tggtaatgac
     6361 ctcttcgccg agatcaataa aatagataac gccttgatta tccagcgtac accacaggca
     6421 gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg ggacatttct ttaactgttg
     6481 gcgcagagaa tcaagcgccg ctacccacgc tttagccgtc ggacgcccgg ttgccacgcc
     6541 actttccgtg aatgcctgct gaaacatggc ttcaacatca ctcggtaaca tcgatagcgg
     6601 aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta tctgacgcgt aggcataacg
     6661 gaaatgggta atatccgtct ccagcgcatt acccgcatca gagataagcg gcacaccaga
     6721 ataaggatgc cggccaccaa acaagacgtg aaaaatcagc aacgcaaggc caaaattacc
     6781 gtgattttct gtgcgttcaa agccgacaaa tgacgacaag gtttgtagct ctggcggcgt
     6841 aaaatgcgac acgccgactt cgcataaatg cagtgtgcca ttggcgttaa tctgaaagga
     6901 gtcgctatca atcaacacca ctttgctgtc gcgacctacc ataaaactgt tctggtttac
     6961 gtcacccacg acatgcccgt gctcgtgaac cgtagcaaaa gatgaagcaa tattgcgcgc
     7021 aacatagagt agaaaatccc acgcacaatg agggtaacgt tgacgacgat gtgccgggct
     7081 atagatcata tgaatcggtt ctttaccaga aacttttggc atcataaaac cgataacttt
     7141 tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg acataattca acaattgcgc
     7201 gtcagctgtc gcagccataa aggcaagttt gtcctgtttt aaggcgggtg gcggcgtgtg
     7261 ataaatcttg gcgacgctat cgacaaactc attgatatca taaaccgcgc cttcaccgcc
     7321 tttgcccagt tcacggcctg gcgtaacgca ctcgccagta gcagtatata aagtgggttt
     7381 catggtaacc acagcgccag tgccaacgtt ttatcatcat ccgtacgctc gtttaccgcc
     7441 gggctggaca aaaactgctt taacaattca ggtaataaat cgagttgttc ctgcgttgct
     7501 gacgccagtc cattgaaaaa cggggtaaag aaaggcacat gcggagaatt atccaacata
     7561 tttaacgcca gtcgctggat accatccgta aatgctgcaa ctttatgcac acgctcagtg
     7621 ctggtgaaag tttccagtct ggaaacggca tcttcatcgg taatgaagtg cgtcatattg
     7681 gcatattcac ccaccatcgg cgtaagcggc aattgcagac cgtgaccaaa atccaccacc
     7741 acgccgccat cgccaatctg cataatcaag gtgccattag ccgatgaaat caatcccaga
     7801 aacgtgcagg caaaatcgcg aactgctaat tctttggctt ccgcctcggc aaaaagtcgc
     7861 tgacgaacgg tcagcacaat atccgtggcg aggatatcat taagccccaa ttccccaccc
     7921 tgcacttttt gcgacatata agccatcgct tcattgacgg cgagcatcgc gccttcgccc
     7981 ccctgcgaaa cgctacctgc gccatcagca aggaacatca ccagtaatgg ctgctggtca
     8041 tttagccagg caacctgcat ctggcaggca tcctggcaag gcaagtccgc gctgatgtgc
     8101 gacgttccaa cagcggatgc ataaaccaga cgccagctca cactgacgtc cagccttttg
     8161 gcgcttccag cacaacttcc gttcccggcg tggagcggga aaccgaacgc agcgaactgg
     8221 ataaccagct aaacagttca cggaattgta atccttgcag aggcaaaggc tgacgaacgc
     8281 tgatttgtgc cagagtcttc atatccgccc cctgaacacc gatggaaaag aaagcaaacc
     8341 ttttatcttc ttccccccga aacactttgt tggctgcagc ctgccactca tcggttggtg
     8401 caccatcggt aatcaggaaa atccacgggc gataatacga aataccattt gcacgatact
     8461 cacgtttccg ctcctctacc atatccaggg ctttggtaat ggccgcaccc attggtgtat
     8521 cgccctgggc aaacaggatg ggcggaaaaa aattagcggc gctggtaaag ggttgttcca
     8581 catgcaccgg gccgaacgtc acaatgccga gttccactct ttttaaagcc agcgaatcgg
     8641 caagcagctc atcgcgaaag gtaaccaatc cggtgttaag ttcattaata ggtctgccac
     8701 tcatcgagcc agaaacatcc agcagcagaa tgcaggggca acgtggttcc gggttactgg
     8761 caaaatcgct ggtggcaaac gtgatttgtt cgctcataag aaatcctttc ttagagaatt
     8821 attgaggtcc ggtatcatac cgactgcaat aatgaaataa gagcaaacga agaaactttc
     8881 agaattagtc ccggcggcgc agaaaatttt gctattcgcc tcgcaagaac ggagagccgt
     8941 cgattgacac taagggcgga gtgacataat ttcaggagtg agggttaggg agaggtttcc
//
LOCUS       DADMGC010000147         8913 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.147, whole genome shotgun sequence.
ACCESSION   DADMGC010000147 DADMGC010000000
VERSION     DADMGC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.147"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(145..2688)
                     /gene="mdoH"
                     /locus_tag="JNZ73_003531"
     CDS             complement(145..2688)
                     /gene="mdoH"
                     /locus_tag="JNZ73_003531"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HAY8733670.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2681..4216)
                     /gene="mdoG"
                     /locus_tag="JNZ73_003532"
     CDS             complement(2681..4216)
                     /gene="mdoG"
                     /locus_tag="JNZ73_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HAY8733671.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPELGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4610..5767
                     /gene="mdoC"
                     /locus_tag="JNZ73_003533"
     CDS             4610..5767
                     /gene="mdoC"
                     /locus_tag="JNZ73_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HAY8733672.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5775..7256)
                     /locus_tag="JNZ73_003534"
                     /pseudo
     CDS             complement(5775..7256)
                     /locus_tag="JNZ73_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
     gene            complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="JNZ73_003535"
     CDS             complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="JNZ73_003535"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HAY8733673.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7826..8137)
                     /locus_tag="JNZ73_003536"
                     /pseudo
     CDS             complement(7826..8137)
                     /locus_tag="JNZ73_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="JNZ73_003537"
     CDS             complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="JNZ73_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HAY8733674.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8649..>8913)
                     /gene="csgA"
                     /locus_tag="JNZ73_003538"
     CDS             complement(8649..>8913)
                     /gene="csgA"
                     /locus_tag="JNZ73_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HAY8733675.1"
                     /translation="NSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNG
                     KNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT         2233 a         2309 c         2111 g         2260 t
ORIGIN      
        1 gcataacgcc ggtattttta ttggctcgtt gtttttgccg gatgtggcgt gaacgcctta
       61 tccgacctac aaaatcttgc aaaatcaata aattgcagga acgatgtagg cctgataagc
      121 gtagcgcatc aggcaactac gtttttattg cgaagccgca tccggtttac gcaatgccag
      181 tggattgagc tttatccctt cgtaataact cacccatgaa gaatatcttt caggggaatt
      241 ccagacgcgg aaatgcagac gggccatcgt caccggatcg cttagcagca ccaggcgacg
      301 atcgcgattc agcttctctg gcgtctcgtt aagcgcctgt tcaacgtggc ggtcacgggc
      361 gatttccagc accttactgg cgcggtgacg cgctgtcgcc attgcggttg ccagagcgtt
      421 aaatgacggg ttaaacactg cgtgcataaa gccatcgtca agggagcgtt ggcgattcat
      481 ctcaaggaac cgatcggtat caaccagcac ctgcggcggt gaatactctt ccgggatcag
      541 gaacagtttc cagcgtttgg tgcgcagacc aacggtggca cggctggaaa tcaccgaaac
      601 aaacggtgac aggatcaacg agaagacaat cggtgccagc cagaacagga aacgcagatc
      661 cagccacgcc atcccaacag cccacactaa ccccaacagc agctgtgagc cgtggcgttt
      721 gaacgcttcg ccccatgaag tggagtcatc atcacgctgc ggtgaattcc acaccacttc
      781 ccagccaagg aacgcgctga caacgaagac cgtatggaac agcatgcgta ccggagccag
      841 cagcacggaa aacagcactt ccagcagcaa cgataatgta acgcgccaga agccgccgta
      901 ttctttcgtt cctttgcacc agataagcaa aatgctcaat aacttcggca ggaacaacag
      961 caccatggtc gaagcaaaaa gtgcaatcgc cagctcagga cgccactgcg gccacaccgg
     1021 gaacaactgc cgtggttgca ggaagtattg cggttcggtc aacgcatgca ctacctgcaa
     1081 tgcagtagag agcgcgagga acataaacca cagcggagcg gagagataag acatcacgcc
     1141 cgtcaggaac accgcacggt gaaccgggtg cataccctta accaggaaca gacggaagtt
     1201 catcaggtta ccgtggcacc agcggcggtc acgttttagc tcatcaagca agttaggcgg
     1261 caattcttca taagaacccg ggagatcgta agcaatccag accccccaac ctgcacggcg
     1321 catcaacgcc gcttctacga agtcatgtga caggattgaa ccggcaaagg aaccttcgcc
     1381 cggcagcgga gccagtgcgc agtgctcgat aaatggtttc acgcggataa tcgcgttatg
     1441 tccccagtag tgcgactcgc caagttgcca gaagtgcaaa ccggctgtaa acagtggccc
     1501 atacacgcgg gtcgcgaact gctgacagcg cgcatacagc gtatccatac cggacgcttt
     1561 cggcgacgac tgaatgatcc cggcgttcgg gttggcttcc atcaagcgca ccaggccgca
     1621 taaacaatca ccggtcatta ccgagtcagc atccagcacc accatgtagc tgtactggct
     1681 gccccagcga cggcagaagt catcgatatt accgctttta cgcttcacgc ggcgacggcg
     1741 gcggcgatag aaaatctgac cttcgccacc gacttcagcg ataagctcca tccaggcttt
     1801 ttgctctgcg acgcagatat ccgggttata actgtcactc agaatgtaga catcaaagtg
     1861 tttggcattc ccggtggctt ttactgattc ccacgttgca cgcaagccag caaaaacacg
     1921 gttcacgtct tcgttacaga taggcatgat caacgccgtg cgatgctccg ggtttaatgg
     1981 ttcatcgcca actgttgacg cagatatact gtatttatcg cgaccaataa gcagttgcag
     2041 gaagcccatc aacgccgtcc agaatccggc ggacacccaa cagaacagta ccgcaaaaag
     2101 gatcaggata ccggtttgca gcatataagg cagaagctgc ataaaggaaa cccacaaatc
     2161 ctgaccaacc atatccatag gattaatcag cgcccaaccc tgataaggaa gaatggtctt
     2221 catataccag gtcgcgacaa cagtttgcgc gagcgtcagg atcaacagaa tgtaacggcg
     2281 gatggtacca acggtacgcc acttttgctc gctctcctgc tcttctttgg tcaaacgagc
     2341 cagatagcgc ggcgtgacat cgcgtccacg caggcgatcc cagaaacggc ctaccgggtt
     2401 ggtacgccac gggtcgggaa acatcgagga gcgttttgct tctggcatcg ccttaagctg
     2461 atcgcgccct tcgtcgtctt taattaactg tccatcagca agtgaatctg gccaggcttg
     2521 ttccagacgc gcctttaccg agccttgcgg ggaatcatcc tcccgcgccc aggtgcggtg
     2581 ttcggcatcc agcgcctgat gaacggcgcg gatatcagtc ttcggcaatg ctgctttctc
     2641 gcttgcggcg atgggcattg cgtcaatgta ctcagttgtc ttattcattg gcaggtaact
     2701 ggtagctcca ggtttcactc aacgtctgat cggcattcac cagcgcagca cgcatttcag
     2761 tggttttctt ggcatctttc actttcacac gcatcaccag acgccagcct ttggtaaccg
     2821 ggttatagcg caccgtgctt tcaactatct caccattatc accaatgctg gtttgcgctg
     2881 tgaccggggt atcctctggc agttttttca tctctgcgcc ggtaaaatcg accacaaagg
     2941 cgatagtacc gtcaggctgg cgaatcaggt tcgactgctt cacatccccc gttgaacgac
     3001 gcgtttgttg cacccatgcg ttatctggcg catgcagttt gtcttcatca cggctgaagg
     3061 tgatggtgta tttaaagttc atctctttac ccagctccgg cagctgatcc ggcgtccagt
     3121 aagcgacgat gttatcgttg gtttcatcgt tggttggaat ttccaccagc tcaacgctgc
     3181 ctttgcccca ctcccctttc ggagtcaccc atgcgcttgg acggagatcg taacgatcat
     3241 cgagatcttc aaagcgggag aaatcacgac ctcgctgcaa cagaccaaag cctcgcgggt
     3301 tttccatgga gaagctgctg accgctaaat gtttcgggtt attcaacgga cgccagatcc
     3361 attcgccatt accagcatgg atagacagac cgttagagtc gtgcaactcc ggacgatagt
     3421 tatttgcagg cgacggttgg ttcggcccaa acaggaacat actggttaac ggtgcaaccc
     3481 ccagtttgcc gactttatcg cgcagataga ttttcgactg cacatccaca accgtgtcac
     3541 gccctggcat aaccacgaat ttgtaagcac ctgtcgcacg cggcgagtca agcaatgcat
     3601 aaatggttaa acgtttatca gtcggttttg gacgctcgat ccagaactct ttgaagcgtg
     3661 gaaattcttc acccgatggc aaagcggtat caattgctag gccgcgggca gaaaggccat
     3721 aaacctgacc tgcaccaatc acgcggaaat agctggcccc gagcatgctg acgatttcat
     3781 cgtttttatc tttgctgttg atcgggtaaa gcactttaaa accggcaaaa ccaaggtctt
     3841 ttaccgtgtc tttgtcatgc tgaacatcgc cgaaagtgaa ataatccggg ctgtatttga
     3901 ttcgtttgac tgcggtggca gtcacttcat ttattttgac cggggtatcg aagtacatac
     3961 cctgatggta gaactcgagt ttgaatgggg tcttcagatt gttccagtac gctttgtcat
     4021 gattaaactg gatctgctga tagtccgcgt atttcatgtc gcggaaaacg gagggcaagt
     4081 tgcttttagg cgcctcatag cctttcccgg ctaaggattg agcttgcttt gcgacatcat
     4141 caatactgaa agcccagctt gaagatgtat acagggttaa cattactgca gcactcaacc
     4201 aacgcatttt catcatttgt agtttatgtt tcataattag taagcacttc cccctttgtg
     4261 tgcttatatc gatccgatct attttaatgg ataaccagcc attccgacaa ctgaatcccg
     4321 gtatcgttca gcacgctggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc
     4381 gtgcttctcc taaagacaga agcatcgact tcgtgtaggg tttgccagca aatttattta
     4441 tcaaccgtct tacagacgct tccattctgg ttagtggtag cgatgatgct atgcataaaa
     4501 accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg
     4561 cataacccga cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt
     4621 acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat
     4681 cccttttcat atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc
     4741 gtcattgtgg ctgacccttt ttaatgactt catccactcg ttccgcatgc aggtattttt
     4801 cgttatatcc ggatactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg
     4861 gaaagtacgt gtcgaacgtg taggtatccc gatgttaaca gccatccccc tactgacatt
     4921 accgcaattt attatgctgc aatatgtcaa agggaaagcg gaaagttggc ctgggctatc
     4981 attgtatgac aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact
     5041 ggtgttagta gtcatgacga cgctgtgtgt atggatattt aagcgcatca gaaataattt
     5101 agaaaattct gataaaacga ataaaaaatt ctcgatggta aaactatcgg tgattttttt
     5161 atgcctcggc atcggctatg cggtaataag aagaacgatt tttattgtgt atccacccat
     5221 tctgagtaat ggcatgttca attttattgt catgcaaacg ctattttatt taccgttctt
     5281 tatcctcggc gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc
     5341 tcgtggctgt accctcgctg cagcattggc gtttgtcgct tatttactca accagcgcta
     5401 tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg atcaccatgg tcctcggcct
     5461 gtggatggtg aatgtggtct tctcctttgg ccaccgttta cttaacttcc agtcagcgcg
     5521 ggtgacttat tttgttaacg catcgctgtt tatctatctg gttcaccacc cgttaacgct
     5581 gtttttcggc gcatacatta caccgcacat cacctccaac tggcttggtt ttctctgtgg
     5641 cctgatattc gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccgtt
     5701 actgaagttt ttgttctctg gtaaaccggt tgttaagcgt gagaacgata aagcaccagc
     5761 ccgttaagcc acatttacaa taaccattcc acgggcaata tcgacgccag tctgaccata
     5821 acccgcttcc agaaactggt ggcgggttct tttttgagaa taacctcttt ctttgcatga
     5881 cgatcaaccc agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcgtac
     5941 tggctctgaa taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag
     6001 cccatttcag tattgagcaa tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc
     6061 gttttaccat cgatgctaaa agttttagca tgcaggctgg caccggaatt accggttatg
     6121 ccgcgatcgt gtaacgtact gctttgttca cgcgtcggct tgagttcata taattccacg
     6181 ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg catggacgac agcaacatcg
     6241 ttagcggcaa gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag
     6301 agttgcgcca cacctgcgcg tgtcggtaca aaataggaag agataatatc gatgcgttca
     6361 ctgggtgagc ccatgatatc gaacaggcgc tgcggtaaca gtgaatgccg ttttgccttg
     6421 ccctcccctt tcgccggatc atcacttaat aaacgtgtct tcgcccagat aagcggcaac
     6481 gttccatcaa ccagatgatt tataaatgga ctggattcca ttttgcgtaa ataacgatgc
     6541 gtcatggcat cgttatgcca ggaggcgggt aactcgatgc gatccgccat ttcaccttcc
     6601 gggacatcca gcacctgctg taagggtgaa accgatttgc aataccagta gcgggcgaaa
     6661 tcatcggcaa cgtcctctac cacggggcct attgccatga catctaaatc cgaaaaaagt
     6721 cgctcttccc ctgcactaaa ataggcatca ccaatattta gtcctcccac tagggtaacc
     6781 acgccatcga cagtgaaact tttattgtgc atccggcgat tgagacggga aaagtcggtg
     6841 atatatccaa gcggacgtag caagcgaaac gagaaaggat taaaaagccg gacttcaatg
     6901 cgtggatgac tgtcaagcaa gcgtaaaatg tcgtcaagtc cgggcgtatt gttgtcgtcc
     6961 agcaacaaac ggacgcgaac gccacgcttt gctgcggcta acagggcgga aaacagtaac
     7021 cgccccgaca tatcgtccta ccagatgtaa tactgaacat ccagcgtatg ctctgccatt
     7081 tcggccagac gataccgggc ggcaaacgca tccagacttt tctccagagg gaaaagacca
     7141 cactgaccgg gatgttgcga acacagtggc agcaccgcgc tcgccagccg gggcaaatca
     7201 ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca
     7261 gacaaagtat acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggttttaac
     7321 tgcgatttca gccgctgccg cacgagggta accataaacc ccagtactga ttgcaggaaa
     7381 agccactgac gtatagctgt ttgccgccac cagtcgtagg ctattcagat aggcatcctg
     7441 caaaagctgg tcttcgtttt gttcaccacc acgccagact ggcccgacgg tgtgtactac
     7501 ggctttagcg ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc
     7561 ctgctgttgc ctgactttta aacaggcatc cagcagggcc ggacccgctg cgcgatgaat
     7621 ggccccatcg acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc
     7681 aacggccagt ttggtaatat caccctgcac aacatgaata cgcgttttca taacgcctcc
     7741 ttacaccttc ttgtttctta agtgtacagc aggctttttg gttaaatcgc atttttctgc
     7801 tcagcaacgt gagttgttca gaaacttagt tatattcgat gttcattaca gccagctttt
     7861 tctgtttatc aagatactgc acttttactg aggctatttg cgcgtttttg acattgccgc
     7921 tcattaacgc tttgctggag taggttttgc ccggaataga accattctat gggcagttca
     7981 tttctacggg tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa
     8041 aatggatcac gcccccttgc gcggtgcttc ctgcaatggc aataaaagga aaaaacaaac
     8101 tgccgagcat aagagagtct aaaaaaggtc tgaacattta ttctatttcc tcaatgatta
     8161 gtcatccttg agggttgtgt tatccatact ttcaggttgg ctgattcgca gcagaccatt
     8221 ctctccagat tcatcttatg ctcgatattt caacaaatta agacttttct gaagagggcg
     8281 tccattgttg tgataaatga agtgcctgtc catcagaaac agtaacaact attttcaccc
     8341 gatcttccgg ggaaatattt aaactcaact tcgtcaaagc aatgggttga ttagcaggca
     8401 atgagagggt cttttcttgc ttcgtctgac tttgccctga actgccttcg cgcagggaca
     8461 atatttgtac tctgcacaga caagattgag taagagtaac ttcaggaata atggtgtaca
     8521 tatccccttg ctgggtcgta ttaaaggtta tctggctgga aagtgccgca aggagtaata
     8581 acgcattcat attcttctcc cgaaaaaaaa cagggcttgc gccctgtttc tgtaatacaa
     8641 atgatgtatt aatactgatg agcggtcgcg ttgttaccaa agccaacctg agtcacgttg
     8701 acggaggagt tcgatgcagt ctggtcaact gcagcaccgt tgccaccacc gaactgttta
     8761 acagtcattt cagaattttt gccgttccac tgatcaagag tggcgctgtt accgaagcca
     8821 cgttgggtca gatcgattga gctgtcatct gagccctgac caacatctgc accattacca
     8881 ccgccatgct gggtaatagt caagtcagag tta
//
LOCUS       DADMGC010000148         8761 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.148, whole genome shotgun sequence.
ACCESSION   DADMGC010000148 DADMGC010000000
VERSION     DADMGC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.148"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..366)
                     /locus_tag="JNZ73_003539"
     CDS             complement(<1..366)
                     /locus_tag="JNZ73_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733676.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLIIKNSTIHGMIYSECMTT"
     gene            890..1864
                     /gene="hemB"
                     /locus_tag="JNZ73_003540"
     CDS             890..1864
                     /gene="hemB"
                     /locus_tag="JNZ73_003540"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAY8733677.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1970..2821)
                     /gene="tauD"
                     /locus_tag="JNZ73_003541"
                     /pseudo
     CDS             complement(1970..2821)
                     /gene="tauD"
                     /locus_tag="JNZ73_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
     gene            complement(2806..3645)
                     /gene="tauC"
                     /locus_tag="JNZ73_003542"
     CDS             complement(2806..3645)
                     /gene="tauC"
                     /locus_tag="JNZ73_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HAY8733678.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVQALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAVTRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQW
                     VNV"
     gene            complement(3642..4409)
                     /gene="tauB"
                     /locus_tag="JNZ73_003543"
     CDS             complement(3642..4409)
                     /gene="tauB"
                     /locus_tag="JNZ73_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HAY8733679.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4422..5348)
                     /gene="tauA"
                     /locus_tag="JNZ73_003544"
     CDS             complement(4422..5348)
                     /gene="tauA"
                     /locus_tag="JNZ73_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8733680.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDDKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            complement(5615..6130)
                     /locus_tag="JNZ73_003545"
     CDS             complement(5615..6130)
                     /locus_tag="JNZ73_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733681.1"
                     /translation="MEQAKEPIVERMAYLIRRYPSRSAAARACGININTLQSYFKKGN
                     TPEPRESILVRIAESEGVSLEWLKTGNGNPPKTQKTQSDLSQGDQLTEMLSFLTSEER
                     EALTNMLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVREILEEIEAS
                     ILSESVNAKAG"
     gene            6323..6574
                     /locus_tag="JNZ73_003546"
     CDS             6323..6574
                     /locus_tag="JNZ73_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY8733682.1"
                     /translation="MRQREDSGRDWPSLMIQQALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            6576..8669
                     /locus_tag="JNZ73_003547"
     CDS             6576..8669
                     /locus_tag="JNZ73_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY8733683.1"
                     /translation="MQSYVTVNDLLGVPGMPATAKGIRQALQRFSRALGDVSRRRAGT
                     KAIEYHIDCLPEVTQKALRERYVEQLVATENNVSEVKTVTRTARNPDAVQAVEAYRGS
                     PQLMEERLNALTENQRRVSEARAALAVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAINANAKRGTNRTISRDPLYQWVLKYNRSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQANGRPMSEAYEDFVTEWQRRYADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEIV
                     GILPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRETLRVLNRD
                     LRSAFNALQQDKPLNARQKSAMRELPSWSELIEAIREGVEWYNNRPHSELPMKPNGKH
                     YSPAEFRKKRLAEENTEIEWLSDLELRDMFCPMVERPVRRCEIKWLGNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFPVSADYYKQQQRIKGMHK
                     RAEEKIRDAVDEGIQVIEQKTTEPWLDNVYRPVGNVMTVQQQEYEEEHDEESERNFRR
                     GLQKLIAMQDDDPLA"
BASE COUNT         2228 a         2254 c         2201 g         2078 t
ORIGIN      
        1 agttgtcatg cactcggaat aaatcatacc gtgaatagtg ctgtttttga ttatcagact
       61 attggcgttg gtattggtag aaataccgtc gtccagatag taggtagaaa tcacgccgtt
      121 aacggtagcg ttattaatga ccgggtagat atcgccgttg taatagctat cagcggcata
      181 gttgttccag cctacataac catcatagta aacgtcatca gcataggtgg cgtcgttgta
      241 gtgatggaaa gtattgtaag tttgaccaga aatatcgttg gtcgctgcat tagcctgaga
      301 ggtgatagcc agagtgcaag ccaatgctaa ttgtgatact acaagtttct ttttccagga
      361 gtgcatttgt catccccctc agggacgtaa taagttgatc catcaattat caatgcatag
      421 aaaacatatt atgtttcgcg ctaacgatta tgctgcgtta cagacgaatg gttcaattat
      481 tctttcccct aagtccgaat gtggacatat ataatttatg gagaaaaaca cgcatcaaat
      541 tgataaaagg acgatatttc agcatgttgc agataatgtt atttttgatg catacgcgtt
      601 ataaatatca aaaacacaaa ccagataaat atcttatcaa tatacaacaa agtttgaatt
      661 attcaattta tagcagcaca accaaacgcc aaaaaaataa taaccaagag ctataaataa
      721 agattaaaaa tattttcagt gaatatgaaa ttattgctat ttctattaca aagatgtcct
      781 ggtctctctt tcattctctt cttgtcaaca tcgcgacaac tttcgtaaaa cactcctgcc
      841 ccgcttcagg tatactgtgc accttaattc cacaaacatc aggcagacca tgacagactt
      901 aatccaacgc cctcgtcgcc tgcgcaaatc tcctgcgctg cgcgctatgt ttgaagagac
      961 aacacttagc cttaacgacc tggtgttgcc gatctttgtt gaagaagaaa ttgacgacta
     1021 caaagccgtt gaagccatgc caggcgtgat gcgcattcca gagaaacatc tggcacgcga
     1081 aattgaacgc atcgccaacg ccggtatccg ttccgtaatg acttttggca tctctcacca
     1141 taccgatgaa accggcagcg acgcctggcg ggaagatgga ctggtggcgc gtatgtcgcg
     1201 catctgcaag cagactgtgc cagaaatgat cgtcatgtca gacacttgtt tctgtgaata
     1261 cacttctcac ggtcactgcg gtgtgctgtg cgagcatggc gtcgacaacg acgcgactct
     1321 ggaaaattta ggcaagcaag ccgtggttgc agctgctgca ggcgcagact tcatcgcccc
     1381 ttccgccgcg atggacggcc aggtacaggc gattcgtcag gcgctggacg ctgcaggctt
     1441 taaagatacg gcgattatgt cgtattcgac caagttcgcc tcttcctttt atggcccgtt
     1501 ccgtgaagct gccggaagcg cattaaaagg cgaccgcaaa agctatcaga tgaacccaat
     1561 gaaccgtcgc gaggcgattc gtgagtcact gctggatgaa gcccagggcg cagactgcct
     1621 gatggttaaa cctgctggag cgtacctcga catcgtgcgt gagctgcgtg aacgtactga
     1681 attgccgatt ggcgcgtatc aggtgagcgg tgagtacgcg atgattaagt tcgccgcgct
     1741 ggcgggtgct atagatgaag agaaagtcgt gctcgaaagc ttaggttcaa ttaagcgtgc
     1801 gggtgcggat ctgattttca gctactttgc gctggatttg gctgagaaga agattctgcg
     1861 ttaatttttt tcctctcaga aagggggagt taacctgttg aaagtacggt gaacgccccc
     1921 tcaccctaac cctctcccca agggggcgag ggaaccgtcc acactcgtat caccccgccc
     1981 gataaaacgg tttatcccca aggatcgtcg cccgatgcat aatccgccgc tgtggcaggt
     2041 aatcggcatt ggcatagtgc tgagtcacgc ggttatccca aatcgcaata tcattcggtt
     2101 gccagcgcca acgcacctga aactccggtt tagtgatatg ggcgaataaa aagcctaaca
     2161 aggcttcgct ctctttctcg ctgacatcga caattcgcgt agtaaagcct tcattcacaa
     2221 acagcgcctg tttaccgctc accggatgcg ttcgcaccac cggatgtagc aacggaggat
     2281 ttttcgcgac cgcctcgcac cagcgttgat gttcctcctc ggttttgcgg tatttgtatt
     2341 ccgggaatga tttacggaaa tcatgctccg cacgcagcca gctcagcagc tggcggaagg
     2401 gaacagagag cgcctcatag gccgcaatac cgctggttca gagcatatca ccgccggtcg
     2461 aaggtaactc tttagctgcc agaatcgccc ctgcgggtgg cgtttcaata aatgtcacat
     2521 cggtatgcca gttgtcgtta tccggcggat tatcgttatg ggtatccagc acgatgatct
     2581 cgtcaacccc ttcggcatga gggtaaacag ggtgaatatg cagttcgcca aaacgctggg
     2641 ccagcgcgcg ttgctgctgc ggcgtaatag cttgatcgcg cagaaatacc acctgatggc
     2701 gcagcaccgc atggtaaagc tgttcaaact gattatcgct taacgggcgc gtcaggtcgg
     2761 cacccgaaat ttgtgcgccg atatgcggcc ccagcggggt aatgctcaga cgttcaccca
     2821 ttgtacttct ccatgccagg gcgtcagacg gcgctgtaac gcgcgcagac ccagttctaa
     2881 aagaaaggcg ataatcgcaa tcaccgcgat ccccgccagt accacgtcag tcgcgagaaa
     2941 ttcacccgct gactgaacca taaatcctaa accgcgtgtc acggcaatca gctccgccgc
     3001 taccagcgta gaccagccca cacccagacc aatgcgtaat ccggtgagga tttccggcag
     3061 cgcgccgggc aaaatgacaa accacagcac ctgcgcacgg ctggcaccca gcgactgggc
     3121 ggcacgaatg cgaacctgct gcgcgctttt cacccctgcc agcgccgaca tcgccaccgg
     3181 cgcaaaaatc gctaaataga tcagtaagat cttcgaggtt tcaccaatac caaaccagat
     3241 caccatcagc ggcaaataag ccagcggtgg caccggacga taaagttcga ttaccggatc
     3301 cagaatgccg cgcaccgtcg ggcttagccc catggcgatc ccgaccggaa taccgatcac
     3361 caccgccgcg agtagcgcct gcacaatgcg cgtcaggctg gcagccagat gctgccacag
     3421 cgtggcgtcc ataaatccct gcggtccggc aatggtgagc agttttgcca gtacttgttg
     3481 cggcggcggc aaaaacagcg ggctaatcag ttgcagcgcc gccaccgtcc accatacagt
     3541 gagtaaaacc gctaacgtgc caatgcttaa ggtcacctga cgcgagagcg gccagcgcca
     3601 tttcagccgc cgcgaatgca gtttttcatt aatgagcaca ctcatgagaa cgcctcccgt
     3661 tgctcaaata cgcggcttaa aacatattcg cgcatggcga tgaattgtgg atcggacttg
     3721 atgctgcggc tcgactctcc cgcaacaaag cggcgagcaa agttgagcgg cagccgctcc
     3781 agcacacggc cagggccgga tgaaagcaga accagttcag tcgccataaa caccgcttct
     3841 tctatatcgt gggtaatcaa cagcacctgc ttgcccgtct cctgccagag tttcagcagc
     3901 agagtttgca tctggtcgcg ggtgaaggcg tccagcgcac caaacggttc atcgagtaat
     3961 aacagctggg gattcgccgc cagcgcgcgg gcaatcccca cccgctgacg ttgaccatcg
     4021 gaaagctgcc agatatagcg tttttctgcg ccttccagcc ccactttttt cagcatctgg
     4081 tgcgcgattt ccagtcgctg cattttctct atacctgcca attgcaggcc gaacgccacg
     4141 ttgtcctgta cattgcgcca cggtagcagc ccttcattct gaaaaactac gccacgctct
     4201 gctcccggtc cctcaatacg cttacccgcc agttgaatgc tgccatgctg ataaggcaca
     4261 aaaccggcaa tcagattcag cagggtggtt ttaccgcagc cggacggccc cagcaccacc
     4321 agtagctcgc cgctttccag cgtcaggttg atatcttcca gtgccggttt gccgccataa
     4381 tcggcataga gatgagagat ttgcagcatc tgcgcctcct tttattgcac gaagcgcgag
     4441 gtaacgtact ggctgtaatc attcgctaca gccgggacct tgccctgctc tttcaaaaac
     4501 tgcgcggtgt cgatgattgc tttgttcacc ggtccggtca gttctgccgt ttgttgctgc
     4561 ggcgtcagat aggtatttcc cttcaccagc cccggaacgt caccttcagg cacgccgctt
     4621 aaacgcgcca gtttgctgat gttttccggc tgtttcagcc acgcgtcagg gttagcaatg
     4681 tacggttgct gtgcatcgat ggcgcttttg gcgaacgctt tcacgacctc aggatgtttc
     4741 tcggcaaaat ctttgcgcac cacccagacg tccagcgttg gcgcacccca ctgcccgacc
     4801 tgttcagaat cggtcagcac cttgtcgtct ttttccaggg cgttaaccgc cggtgcccag
     4861 acataagcac catcaatatc accccgctgc catgcggcga taatcgcggg cggctgcagg
     4921 ttcacaatct ccactagccc tggtttaatc ccccagtgtt tcagcgccgc cagcaggctg
     4981 tagtgggtgg tggagataaa cggtacggca atgcgtttac caatcaaatc ttcgggtttg
     5041 ctgatagttt tctttaccac cagcgcttcg gagttaccca gttttgacgc cagcaagaag
     5101 acttcaatcg gcacctgttg gctggctgca accgctaatg ggctggatcc gaggttgccg
     5161 atttgcacgt cgccagaagc cagcgcccgc acgatgctgg ctccgctgtc aaacttacgc
     5221 cagtccacgc tcgccccgct ctctttagca aaggtgttgt cggcctgagc cactttcgcc
     5281 ggttcggctg aggtttgata cgccacggtg acgttcaccg cctgtgcctg aaaagcgatg
     5341 aatgccagtg cggcaagaag tgtgttatat gactaggtaa tttattcaag ataaccggga
     5401 atgggtaaaa aaagacggga tttgatggga ttcaataagt tatgcgccga ttgcattcaa
     5461 gataaaacga gttaaaaatg ctcaatgctt cgatatcgat aaaacattca agataggcgc
     5521 gccgtgtatt ttaattcttt taagactctt caaaaatgtc atcgtagcct ttaaaaacga
     5581 attttaaagg caccctaaag gcaggtcaat gaagttaacc cgccttcgcg tttacactct
     5641 ctgagagaat actagcttca atctcttcca aaatctctct gacttgctcg tcgggcatat
     5701 caacgagcat caacgctgcc aggcgtttac ggccctctag ttgcagtagc tcttgagttt
     5761 ccttatctaa caagcgtagc acagtctcaa cacctttcct tgcgagcatg tttgtaaggg
     5821 cttctcgttc ctcactggta agaaacgaca acatttctgt tagctgatcc ccttggctta
     5881 aatcactttg tgttttttgt gtttttggtg gattcccgtt accggttttt agccattcaa
     5941 gactaacacc ttcactttct gcaatgcgta ccaatatgct ttctcgaggt tctggtgtat
     6001 ttcccttttt aaaatagctt tgtaacgtat tgatatttat gccacaggct ctggcggcag
     6061 cacttcttga tggataccgc ctaataagat aagccatgcg ctccacgata ggttcttttg
     6121 cctgctccat aaaagtacct ctaggtattt ttctgtataa aggttctttt ccatctatct
     6181 aattgaatgg aaaggtaatt ttaaaaatcc aaataaaata caaaaaaata ccttgtattg
     6241 gtgctttttg tgatctgcga ttttatttac tcggatatcc acgcgaatat ccgcgcgggt
     6301 aattttgtag ggtaaaggaa agatgagaca aagagaagat agtggccgcg attggccttc
     6361 gctgatgatt cagcaagcac tcaaagataa tgggtggtct tcctttcggg agctttcact
     6421 ccatgccggt tatgctcagg attcactgaa agcggtagtt cgtacgccaa acaaaaaata
     6481 cgaacgtgtt atttctgacg ctataggtgt accgccagaa atcatctggc ccagccggta
     6541 tcgtaacgat ggatttatca ggaaggcgtc gtgatatgca gtcgtacgtt accgtaaatg
     6601 accttctcgg tgttcctgga atgccagcca ccgcgaaagg catccgacag gcgttacagc
     6661 gtttttctcg tgctcttggt gacgttagtc gccgtcgagc aggtacaaaa gccattgagt
     6721 accatatcga ttgcctgcct gaggtaaccc aaaaggcact gcgtgaacgc tatgtggaac
     6781 aactggtggc tacagagaac aacgtctccg aagtgaagac cgttaccagg acggcgcgta
     6841 accctgatgc tgttcaggca gttgaagcat atcggggttc tccgcaacta atggaagaac
     6901 gcctgaatgc cctgactgaa aatcagcgcc gggtgtctga agcaagagcg gcgctggcag
     6961 tggaagtgct gaagctggaa agcgccggta acctcgggcg actgaaagcc attaactttc
     7021 tggttgaaaa agcccgtaaa ggtgagttgc cggaacgact gcaacaggct gcgattaacg
     7081 ctaatgcaaa acgtggcact aatcgcacca tcagccgtga tccgctttac cagtgggtac
     7141 tgaaatacaa ccggtcgcag aacgccgcag aacgactgtt gttactggca ccaggaaaac
     7201 gcgacgaaat aaaaccggaa gaaattagct ggctgcctga atttctggcg cagtatcgtc
     7261 aggctaacgg cagacctatg tcggaagcct acgaggattt tgtcacggaa tggcaacgtc
     7321 gctatgcgga cgaaccgtac atgctggagg tgatgccttc ctatgatgtt gtccggtatg
     7381 ccatgaaaaa attgccggaa gtggtgaaac aaaaaggacg cgttaccggc agtgaatacc
     7441 gccagcttga gggattcacc cgccgcgact ggacagctat gccggtgaat tatgtctgga
     7501 ttggtgacgg ccacggtatg aagctgaaat gtgcgcatcc gatccacggg cgtccatttt
     7561 caccggaagt cacgtttgtt atcgacggtg gaacccggtt tgttgttggc tggagtcttg
     7621 atcttgcgga gaacgtgttt gctgttgccg gggcgataca gcacggaatt cgcaatcacg
     7681 gaaaaccctt cctgtattac tcggataatg gttccggtga aacggcagat atgctggata
     7741 aagagatcgt cgggattctg ccccgtctgg gtattaacca ccccaccggt attgccggaa
     7801 atccgcaggg acgcggcatc attgaacgtc tgaaccggac gctgccgatg cgtatagcca
     7861 gacggtatcg cacctatttt ggcaaggggg cagaccggga aacactgcgt gtactcaatc
     7921 gcgatctgcg ttcagcattt aatgccctgc aacaggacaa accgctgaat gcccgccaga
     7981 aatctgcaat gcgcgagctg ccgtcctggt cagaactgat tgaggctatt cgtgaaggag
     8041 tggaatggta caacaaccgc ccgcattctg aactgccgat gaaaccaaac gggaagcatt
     8101 acagcccggc tgagttcaga aaaaaacgcc tggcggaaga aaacaccgaa attgaatggc
     8161 tgagtgatct cgaactgcgc gacatgttct gcccgatggt ggagcgtccg gtaagacgct
     8221 gtgaaatcaa atggcttggg aatatttatt acgcgccgga gctgcgtgat gaacatggtc
     8281 gcaaagtgct tatcagctat gacattcatg atgcagagcg aattacagtg cgccgtaagg
     8341 atggcagttt tatttgtgag gcgatatgga acggcaataa acgcgccgcc ttcccggtca
     8401 gcgccgacta ttacaaacaa cagcagcgta ttaaaggaat gcacaaacgc gcagaagaaa
     8461 aaatccgtga tgccgtggat gaaggtattc aggttattga acaaaaaacg acagaaccct
     8521 ggctggataa tgtttatcga cccgttggca atgtgatgac cgttcagcaa caggaatatg
     8581 aagaggagca cgacgaagaa tccgaacgca atttcaggcg gggattacag aaactcattg
     8641 ccatgcagga cgatgaccca ctggcctgaa taaaaaaaca gtccgcagca acggactgtt
     8701 aaacaggtaa ataaagctga tttgaaataa ccgaggaaac aatatgaccg atattaatga
     8761 t
//
LOCUS       DADMGC010000149         8711 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.149, whole genome shotgun sequence.
ACCESSION   DADMGC010000149 DADMGC010000000
VERSION     DADMGC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.149"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(13..753)
                     /gene="cysQ"
                     /locus_tag="JNZ73_003548"
     CDS             complement(13..753)
                     /gene="cysQ"
                     /locus_tag="JNZ73_003548"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HAY8733684.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            943..2886
                     /gene="cpdB"
                     /locus_tag="JNZ73_003549"
     CDS             943..2886
                     /gene="cpdB"
                     /locus_tag="JNZ73_003549"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HAY8733685.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3012..3392)
                     /locus_tag="JNZ73_003550"
     CDS             complement(3012..3392)
                     /locus_tag="JNZ73_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY8733686.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3481..4341
                     /gene="qorB"
                     /locus_tag="JNZ73_003551"
     CDS             3481..4341
                     /gene="qorB"
                     /locus_tag="JNZ73_003551"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY8733687.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAALVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            4450..5415
                     /locus_tag="JNZ73_003552"
     CDS             4450..5415
                     /locus_tag="JNZ73_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY8733688.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5523..6185
                     /gene="ytfE"
                     /locus_tag="JNZ73_003553"
     CDS             5523..6185
                     /gene="ytfE"
                     /locus_tag="JNZ73_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HAY8733689.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6230..7642)
                     /gene="cycA"
                     /locus_tag="JNZ73_003554"
                     /gene_synonym="dagA"
     CDS             complement(6230..7642)
                     /gene="cycA"
                     /locus_tag="JNZ73_003554"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HAY8733690.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDFSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(7950..8570)
                     /locus_tag="JNZ73_003555"
     CDS             complement(7950..8570)
                     /locus_tag="JNZ73_003555"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="FKBP-type; rotamase; catalyzes the cis-trans
                     isomerization of proline imidic peptide bonds in
                     oligopeptides; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY8733691.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALIAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT         2211 a         2295 c         2207 g         1998 t
ORIGIN      
        1 tgccatctga atttagtaaa tggacactct gaaccccgga ttcaggaacg actcgcgcgg
       61 agtgtaatcc agcggtttac cctgccagtc gtgaacgtgc gctccggcag ctgcagccac
      121 agcatgtcca gcggcggtgt cccaaatatt cgttggtccg aagcgcgggt acagctgcgc
      181 ctgtccttcc gccaccaggc agaatttcag cgaagagccg atggacgtgg tctgatgttc
      241 gccaagctgt tgcagatact ctttcagctc cgcatctgca tgggaacggc tgataaccac
      301 cagcggcggg cgcgcatcgc ggacctgaat cagcttgcgc acaccgcact cttctttcca
      361 ggccttgcct tctgccgcgc tgtacattac gttcattacc ggcgcataca ccacacctaa
      421 aatcggtttg ccatggtcaa tgagcgcaat gttaacggtg aattcgccat tacgtttaat
      481 aaactcttta gtaccatcca gcgggtctac cagccagtaa cgctgccagt gctgacggac
      541 ttcccaaccg ggaggatctt cttcagaaag gaccggaata tccggtgcca gcgtacgtaa
      601 accgtccatg ataacggtgt gagcggcaat atccgctgcc gttaccggag aattgtccgc
      661 tttgctgacg acgtccatcg gtttcgtccc gtcgtagacc tgcataatgg catcgcctgc
      721 attccgtgca agctggcata cttgatctaa catttctcca cctcgtctct gtgagcggtg
      781 ttaacttatt gttttactta taccctatcg ttaatgaatg cgccaactgt gatagtgtca
      841 tcattttcaa agcgtaaaat tgtggcattc ttcactgttc tataagtaag gcgtttattc
      901 tcccttttct ttcgtattcc cgatgataaa aggatgtccc tgatgattaa gtttagcgca
      961 acgctcctgg ccacgctgat tgccgccagt gtgaatgcag cgacggtcga tctgcgtatc
     1021 atggaaacca ctgatctgca tagcaacatg atggatttcg attattacaa agacaccgcc
     1081 acggaaaaat tcggactggt acgtacggca agcctgatta acgatgcccg caatgaagtg
     1141 aaaaacagcg tactggtcga taacggcgat ttgattcagg ggagtccgct ggccgattac
     1201 atgtcggcga aaggattaaa agcaggtgat gttcatccgg tttataaggc gctgaatacg
     1261 ctggattata cggtcggtac actcggcaat catgaattta actacggtct ggattacctg
     1321 aaaaatgcgc tggcgggagc gaaattccct tatgtaaatg ccaacgtcat tgacgccaga
     1381 accaaacagc caatgtttac accgtattta attaaagata ccgaagtggt cgataaagac
     1441 ggaaaaaaac agacgctgaa gattggctat attggcgtcg tgccgccgca aatcatgggc
     1501 tgggataaag ctaatttatc cggaaaagtg acggtgaatg atattaccga aaccgtgcgc
     1561 aaatacgtgc ctgaaatgcg cgagaaaggt gccgatgtca ttgtcgttct ggcgcattcc
     1621 gggctgtctg ccgatccgta taaagtaatg gcggaaaact cagtttatta cctcagtgaa
     1681 attccgggcg ttaacgccat tatgtttggc catgctcacg ccgttttccc gggtaaagat
     1741 tttgctgata tcgaaggggc tgatatcacc aaaggcacgc tgaatggtgt tccggcggta
     1801 atgccgggca tgtggggcga tcatcttggg gtggtcgact tacaactcag taatgacagc
     1861 ggtaaatggc aggtgacgca ggcgaaagcg gaagcacggc cgatttacga catcgccaat
     1921 aaaaaatccc tcgcggcgga agacagcaag ctggtagaaa cactcaaagc cgatcacgat
     1981 gccacacgcc agttcgtcag caagccaatc ggtaaatctg ccgacaatat gtatagctat
     2041 ctggcgctgg tgcaggacga tccgaccgtg caagtcgtga acaacgcgca aaaagcgtat
     2101 gtcgaacatt acattcaggg cgatccggat ctggcaaaac tgccggtgct ttcagctgcc
     2161 gcaccgttta aagttggtgg tcgcaaaaat gacccggcaa gctatgtgga ggtggaaaaa
     2221 ggccagttga ccttccgtaa tgccgccgat ctttatctct accccaatac gctgattgta
     2281 gtgaaagcca gcggtaaaga ggtgaaagag tggctggagt gctccgccgg acagtttaac
     2341 cagattgctc ctaacagcac gaaaccacag tcactcatca actgggatgg tttccgcact
     2401 tataactttg atgttattga tggtgtgaat tatcagattg atgttaccca acccgcccgc
     2461 tatgacggcg agtgccagat gattaatgcc aatgcagagc ggattaagaa cctgaccttt
     2521 aatggcaaac cgattgatcc gaacgctatg ttcctggttg ccaccaataa ctatcgcgct
     2581 tacggcggca aatttgccgg tacgggcgac agccatatcg cttttgcttc accggatgag
     2641 aaccgctcgg tgctggcagc gtggattgct gatgagtcga aacgtgcggg ggaaattcat
     2701 ccggcggcag ataacaactg gcgtttagca ccgatagctg ccgataagaa actggatatc
     2761 cgtttcgaaa cttccccgtc agataaagcc gcagcgttta ttaaagagaa agggcagtat
     2821 ccgatgaata aagtcgcgac cgatgatatc gggtttgcga tttatcaggt ggatttgagt
     2881 aagtaaaaca cttctttttc ggcctataaa tcaaccgcat ccggcattta ttggcggatg
     2941 cgatgctgcc gtatcttatc caccctacaa gccatgaacc gtagaccgga taagcgcagc
     3001 gcatccggca gttatgccgc acgttcatcc cgcaccgcca acacctcggg caaattcaac
     3061 tcaatccagt ccgccagtgc agcaaccttt tcgctcacct gctcgcccag cggcgtgagg
     3121 ctatattcca catgcggcgg caccaccgga tacgcgatac ggttaagaaa accatcctgt
     3181 tctaacgcct gtaacgactg cgcaagcatc ttttcactca ccccaccgat tttgcgccgc
     3241 aggtcgctaa agcgatgagt accttcgcgt agcgccacca gaatcaacac cccccaacgg
     3301 ctggtgacgt gtttcaacac ctcgcgcgac gggcactgtt ccgcaaagag attaccctct
     3361 ttcagttgtt gtgacaggct aacctgactc atttcatact tacctttttg cacgtactta
     3421 ctaaaagtaa gtttaggtgt tagcgtattt aaacacaaga caaaacgatg gagacttccc
     3481 atgatcgcta ttactggtgc cactggccaa cttggtcact atattattga atccttgatg
     3541 aaaacggttc ctgccagcca aatagtggct atcgttcgta atccggcaaa agcccaggcc
     3601 ctggcagcac aaggcattac cgtgcgtcag gctgactaca gcgatgaagc cgcactgaca
     3661 tctgcacttc agggagtgga aaaactactg ctgatctctt ccagcgaagt gggtcaacgt
     3721 gccccgcagc atcgtaatgt tattaatgcc gcaaaggcgg ctggcgtgaa atttatcgct
     3781 tataccagcc tgctacatgc agatacctcc ccgctcggcc tcgccgatga gcacatcgag
     3841 acggagaaaa tgctggctga ttctggcatc gtttacaccc tgctgcgcaa cggctggtac
     3901 agcgaaaact acctcgccag cgccccggca gcactggaac acggcgtatt tatcggtgcg
     3961 gcgggcgatg gcaaaatcgc ctcagcaacg cgggcagatt atgcgacagc tgcggcactc
     4021 gtgattagcg aagccggtca cgaaggcaag gtttacgaac tggcgggcga tagtgcctgg
     4081 acgttgacac agttagcggc agagctgacc aaacagagcg gcaaacaggt tacctatcaa
     4141 aatctgagcg aagccgattt cgccgcggca ctgaaaagcg tcggactgcc cgacggactg
     4201 gcggatatgc tggcggattc tgacgttggc gcatcgaaag gtggtctgtt tgataacagc
     4261 aaaacgctta gcaaattgat tggccgccca acgacaacgt tagccgaaag cgtaagccat
     4321 ctttttaatg ttaataacta gttaattaaa gtggcatcct cccgcatcct ctctgataat
     4381 gacgggatgc cgggagcaat catgtctgct tcctgaacgt ttcttctgac agaccaatgg
     4441 atgccagtaa tgattagcgg cgtgctgtac gccctgttag cagggttgat gtgggggctt
     4501 atttttgtcg ggccgttgat cgtgccggaa tacccggcga tgttacagtc gatggggcgt
     4561 tatctggcgt tagggttaat tgcgctaccc attgcctggc tgggacgcgt gcgtctgcgt
     4621 cagttggcgc gtcgggactg gcttaccgcc ttgatgctca ctatgatggg caacctcatt
     4681 tattacttct gccttgccag tgccattcaa cgtactggcg cgcctgtttc cacgatgatt
     4741 atcggcaccc tgccggtggt gattcctgtc tttgccaatc tgctttatag ccagcgcgac
     4801 ggcaaactcg cgtggggaaa actcgccccg gcactgattt gtattggcat cggcctggcg
     4861 agtgtgaata ttgctgagtt aaaccacgga ctccccgatt ttgactgggc acgttatacc
     4921 tctggcatcg tgctagcgtt agtttccgtg gtctgctggg catggtatgc cctgcgcaac
     4981 gcccgctggc tgcgggaaaa tcccgacaaa catccgatga tgtgggcgac ggcgcaggcg
     5041 ctggtcacgc tgccggtttc tctcatcggc tatctcgtcg cctgttactg gctgaatata
     5101 caaacgccgg acttctcctt accttttggc ccccgtccgc tggtgtttat tagtctgatg
     5161 gttgcgatag ccgtgctttg ctcatgggtt ggcgcactct gctggaacgt cgccagccag
     5221 cgattaccga cagtgattct cgggccgctg attgttttcg aaacgctggc aggtttgctg
     5281 tacacctttt tactccgcca gcaaatgccg ccgctaatga cgctgagcgg tatcgcgctg
     5341 ttagtgattg gcgtggtcat cgcggtcaga gcaaaaccag aaaaaccttt aactgaatct
     5401 gtctcagaaa gttgacatgc tggcagtgag ttaaataagc ctctgctacg taagggttat
     5461 agcttttgcc ttaaaggtgt atttaaaata catcttatct tattaagaat gaggtatcag
     5521 ctatggctta tcgcgaccaa cctttaggtg aactggcgct ctctatccct cgcgcttcgg
     5581 ctctgtttcg taaatatgat atggattact gctgtggcgg taagcagacg ctggcgcgcg
     5641 cggcggcacg taaagaactg gatgttgagg tcattgaagc tgaactggca aagcttgctg
     5701 aacaaccgat tgagaaagac tggcgtagcg ccccgctggc agaaatcatt gaccatatca
     5761 tcgtgcgcta ccacgatcgt caccgcgagc agctgccgga gctgatcctg caagcgacta
     5821 aagtcgagcg cgttcacgcc gacaaaccga gcgtgccaaa agggctgaca aaatacctga
     5881 ccatgctgca tgaagagctt tccagccaca tgatgaaaga agagcagatc ctcttcccga
     5941 tgatcaaaca aggcatgggc agccaggcaa tggggccaat cagcgtaatg gaaagcgagc
     6001 acgatgaagc gggcgaactg ctagaagtga tcaaacacac taccaataac gtcacaccgc
     6061 cgccagaagc ctgcaccacc tggaaagcga tgtataacgg cattaatgaa ctgattgatg
     6121 acctgatgga tcacatcagt ctggaaaaca atgtactgtt cccacgcgcc ctagcgggtg
     6181 agtgatctaa aagctggatg gcattgcgcc atccagcatg ataatgcggt tatttccgca
     6241 gttcagcagc ccgcttctta ccaataaaca gccagcccaa ccccagcgcg ataaaccaca
     6301 gcggggtaac cagcagcgcc tggcgagtgt catcttccag tgtcagcaac acgaccacga
     6361 acacaaagaa cgccatacat acccagcaca tcagcttgcc gagcggcatc ttgtagatcg
     6421 acttttcatg cagatgagga cgctgtttgc ggtacaccag gtacgagcaa aggataatcg
     6481 tccagacgaa cataaacaga atcgcggaaa cggttgtaat catcgtgaac gcgccaatca
     6541 cgctaggatt cacatacaac atcaccacgc cgccgagcag acagatacac gagaaggtca
     6601 accctttcgc gggtactgcg cgcttagaaa gtttagcgaa cgctttcggc gcaacacctt
     6661 cctgcgccag accaaacagc atacggctgg tagagaagac accgctgtta gcggaagacg
     6721 ccgcagaggt cagcaccaca aagttgatca cgctggcagc cgcaggcaaa cctaccaaca
     6781 caaacagctc aacgaacggg cttttctccg ggactaccga actccacggt gtcacggaca
     6841 taatcacaat cagcgcgaag acgtagaaca taatgatacg gatcggaatg gagttaatcg
     6901 cgcgtggcag tgatttctcc ggatctttgg tttccgcagc tgttgtacct accagctcaa
     6961 tccccacgaa agcgaaaacc gctatctgga atccggcaaa gaagccactt aaacctttcg
     7021 ggaaccagcc gccgtcattc cacaaatgcg cgaatgacgc ttccacaccg gtcggtgact
     7081 gaaagtgcat cgccaccatg accaggccga cgacaatcag cgacacgatg gcgacgattt
     7141 tgatcatcgc aaaccagaac tccatctcac cgaacatttt cacggtggca aggttgagcg
     7201 tcagcagcag cactatcacc gccagcgagg cgacccagtc ggagaaatcg gggaaccaga
     7261 actgagcata agccgtgatc gccaccacgt ctgccatacc ggttacaacc cagcagaacc
     7321 agtaagtcca gccggtgaaa tatcccgccc acggccccag taaatcggaa gcgaagtcac
     7381 taaaagattt gtattccaga ttcgaaagca gcaattcccc cattgcccgc atcacgaaaa
     7441 agagcataaa accaatgatc atataaacga aaatgatcga cggcccggca aggctaatcg
     7501 ttttgccgga ccccataaac aacccagtac caatggcacc gccaatggca ataagctgaa
     7561 tatgtcggtt tgtgagattg cgccgtagcg actgttcagc cggagcctga tcatcggcaa
     7621 cgacttttac ctgatctacc atgttttttc ttcctgtacc tgtctgtgtt gttcaggctc
     7681 tacggccttt agtcagtcta tgatacgacg aatctgtatc gggactcatc gatattaggt
     7741 aacaatacgc gggatgaata ctaagattta gatctaatgt taattttatg tttaaagtga
     7801 gtgtcatatc acggtgataa cgcgaaacag ctcacaaaaa tacacgctta cggggtcttt
     7861 gcaacacaaa atagcgccgt ttggcaaact gaagggttta ttgctgaatg cctgctcccc
     7921 tctggttcga ggggaacaga atgcgctgct tagaggattt ccagcagttc gacttcaaac
     7981 accagggtgc tgaacggagg gatggatgca cctgcgccgc gctcgccata tgccagttcc
     8041 tgcgggatag tcagttccca tttagaacct accggcatca gagtcagtgc ttcaatccag
     8101 ccagggatca cgccattaac cgggaattca gcgggttcac cacgagcaac ggagctgtca
     8161 aacacggtgc cgtcgatcag tttaccggtg taatgaacac gaacgcggtc ggtacgtgcc
     8221 ggaattgcgc cttcaccttg gttgatcacg cggaattgca ggccagattc ggtgctattc
     8281 acaccttctt ttttggcgtt ttcttccagg tatttcacac cttcagcagc catcgcctgg
     8341 aaacgctgac gacgaacggc atcggcgcgt tcgtggattt cacgcagcgc gcgatgcacc
     8401 acatcaaccg gcacagccgg atgtttgcct tccagcgcat cggcaatacc tgcaatcagt
     8461 gcttctggca gcagcccttc caggccagat tcactcagtt gttgccctac ctgcaaacca
     8521 atgccgtagc ttgcttgcgc ttcgatggtg tcaaaagttg gggtggtcat cgtatttcct
     8581 ttcttagggt ataaaagtag cgggcagcat atcagccaca cttctttggg taaaactttg
     8641 tcgcaggaat gatgacaaat cccggggaaa aagaaacaat agcaatatcc cgtatgaata
     8701 taataaaggc g
//
LOCUS       DADMGC010000150         8650 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.150, whole genome shotgun sequence.
ACCESSION   DADMGC010000150 DADMGC010000000
VERSION     DADMGC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.150"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..623)
                     /locus_tag="JNZ73_003556"
     CDS             complement(<1..623)
                     /locus_tag="JNZ73_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAY8733692.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDP"
     gene            complement(704..1888)
                     /gene="uxuA"
                     /locus_tag="JNZ73_003557"
     CDS             complement(704..1888)
                     /gene="uxuA"
                     /locus_tag="JNZ73_003557"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAY8733693.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            2228..3571
                     /gene="gntP"
                     /locus_tag="JNZ73_003558"
     CDS             2228..3571
                     /gene="gntP"
                     /locus_tag="JNZ73_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HAY8733694.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(3822..4724)
                     /gene="fimH"
                     /locus_tag="JNZ73_003559"
     CDS             complement(3822..4724)
                     /gene="fimH"
                     /locus_tag="JNZ73_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HAY8733695.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPTNNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(4744..5247)
                     /gene="fimG"
                     /locus_tag="JNZ73_003560"
     CDS             complement(4744..5247)
                     /gene="fimG"
                     /locus_tag="JNZ73_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HAY8733696.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(5260..5779)
                     /gene="fimF"
                     /locus_tag="JNZ73_003561"
                     /pseudo
     CDS             complement(5260..5779)
                     /gene="fimF"
                     /locus_tag="JNZ73_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
     gene            complement(5789..8425)
                     /gene="fimD"
                     /locus_tag="JNZ73_003562"
     CDS             complement(5789..8425)
                     /gene="fimD"
                     /locus_tag="JNZ73_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
                     /protein_id="HAY8733697.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIAHGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSIMAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQFYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(8492..>8650)
                     /locus_tag="JNZ73_003563"
     CDS             complement(8492..>8650)
                     /locus_tag="JNZ73_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone FimC"
                     /protein_id="HAY8733698.1"
                     /translation="ELNAGTRVLENALVPPMGESTVKLPSDAGSNITYRTINDYGALT
                     PKMTGVME"
BASE COUNT         2040 a         2241 c         2153 g         2216 t
ORIGIN      
        1 gggtcacgcg cctgagccag ccccagtacc gcgaccttcg ccacatgacc gttttcacgc
       61 acgttatcgc aggacatcac cgtaaatgct ttcagccctt tttcacgacg cagacgcaag
      121 gcttcgacga tgtaaccaat cgcggacttc ggcgcagtcg ggttttccag atcgtgcttg
      181 atcagcgggt tattgagatc cagttgaccg cttgccgcat cagcgcagta gcctttttcc
      241 gtgaccgtta gagagacaat cgccgtttgc ggacgcgcca tcgcgttgag aataccttcg
      301 cagccgtcga tttccggatg cagcgcttct ttcatcgaac cgataatttt cagctcggtg
      361 ctttctgcgc ctttttccgc tacggtgtac agcagttgct gttttttcag gttttcgatc
      421 agcatacggt cgttgcctgg catcaggtta acttcgcaga tgccccagtc gctgtcggtg
      481 ctttccagca gatgatgggt atacaacgcc tggtgcgcgc ggtgaaacgc cccgcaaccg
      541 agatgcacaa tgcgtgattc cagacgagaa tgatcccatg acgggcgggc aaccggcaga
      601 ttgctgtcaa caatagtagt catcgtaaac tccagaatcc gtcttcagag agtgacggca
      661 aggaagaagg aaccggcacg gcgcgcagcc atgccggtgg gtattaacgg ctaaagaaag
      721 cgcgctggat cgccagttcg acaccgcgaa cttcggccag gcctttcaga cgaccaattg
      781 cggagtaacc agggttggtt ttcttcttca ggtcgtccag catctgatga ccgtggtccg
      841 gacgcatcgg gatcaggtct tctttgcctt ccgctttacg acggtgttct tcttcaacaa
      901 tcgctttcac cacttcgtac atatcaacgt caccgttcag gtgcgccgct tcgtggaagg
      961 ttttcgggtt atcttcacgc atggtggagc gcagatgggt gaagtaaata cgcggaccga
     1021 actgcttgat catatcaacc agatcgttgt cagcacgcac gccgtaggaa ccggtgcaca
     1081 tggtgaaacc gttcgccatg ctgtttacgg tatcaaccat ccactgcata tcttcaatgg
     1141 tggaaacaat gcgcggcaag ccgaggatcg ggcgcggcgg atcgtccggg tgaaccgcca
     1201 tacgcacgcc aacttcttca gcaactggaa taatcgcttt caggaagacg gcaaagtttt
     1261 cgcgcagttt ggctttgtcg atatctttgt acagctccag gtgcttacgg aactggtcga
     1321 gggtataccc ttcttccgcg cccggcagac cggcaatgat gttacgcgtc agacgcgctt
     1381 tgtcttcgtc gctcatagtg gcgaagcgtt cagctgcctg agcaatttct tcttcggtgt
     1441 aatccgcttc cgcgcctgga cgtttcagga tatgcatttc gaatgcagcg aattcgatct
     1501 ggtcgaagcg cagagctttg gagccgtctg gcagcacgta ttcgaggtca gtacgggtcc
     1561 agtcgagcac cggcatgaag ttgtagcaca cggtgcgaat gccacactgc gccaggttgc
     1621 gcagagtctg ctgatagtta gcgatccact gctcatagtt gccagtgtgg gttttgatat
     1681 cttcgtgaat tggtacgctt tctacgacag accacaccag gcctgcgtct tcaacgatcg
     1741 ccttgcgttt gaggatctct tctacggacc atacttcgcc gttcgggata tggtgcagcg
     1801 cggtaaccac gccagttgcg cccgcctgac ggacatcagc taaagaaacc ggatcgtttg
     1861 ggccgtacca gcgccaggtc tgttccatga tttttcctct ttcacatagt gggttccaca
     1921 gcaaagagac gcggaacctt ggagttgttt aggtatccgg atgaacaacc agaccgcagc
     1981 cgtggcgttg catcggatga gatggcgtat aagttctacc ttttggtaag acatgtcaac
     2041 ccgaactctg aaattggtta accacatcac aagaatttca ctctcatggt tgttgctgca
     2101 aattgcagca aagggtaatc gatggttatt gatcagagga tgtgacattc atcgcaacaa
     2161 tggttgacca atttgcatga catgtcgccc aaaataacaa cggttcaacc aagtaaggta
     2221 aaacgttatg catgtgctta acattctctg ggtggtattc ggcattggcc tgatgctgat
     2281 actgaatttg aagttcaaaa tcaattcaat ggtggctttg ttggtggcgg cgctgtccgt
     2341 cgggatgctg gcaggcatgg atttgatgtc gctgctgcac accatgaaag cgggcttcgg
     2401 caacacgctg ggagaactgg ctatcatcgt ggtgttcggt gcggtcatcg gtaaattgat
     2461 ggtcgactcc ggcgcggctc accagatagc acatacgctg ctggcgcgtc taggtctgcg
     2521 ctatgttcag ctgtcggtga ttatcatcgg ccttattttc ggtctggcga tgttctatga
     2581 agtggccttt atcatgttag cgccgctggt tattgttatt gccgccgaag ctaaaattcc
     2641 gttcctgaaa ctggcgatcc cggcagtagc agctgccact accgcacatt cactgttccc
     2701 accgcagccg ggtccggtgg cgctggtgaa tgcttatggc gcggatatgg ggatggttta
     2761 tatctatggc gtactggtga cgatcccaag tgtaatctgc gcaggtctga tcctgccgaa
     2821 gttcctcggc aatcttgagc gcccaacgcc atcattcctg aaagcagatc aaccggtaga
     2881 tatgaataat ctgccctctt tcggcgtttc gattctggtg ccgctgatcc cggcgatcat
     2941 tatgatctct accaccatcg ccaatatctg gctggtaaaa gatacccctg cctgggaagt
     3001 ggttaacttt attggttcct cgccgattgc gatgtttatt gcgatggtgg ttgcattcgt
     3061 actctttggc accgcgcgtg gtcatgacat gcagtgggtg atgaacgctt ttgaaagcgc
     3121 ggtgaagagt attgcaatgg tgattctgat catcggtgcg ggtggcgtgc tgaagcagac
     3181 catcatcgac accggcattg gcgacaccat cggcatgttg atgtcccacg gcaatatctc
     3241 gccctacatt atggcatggc tgatcactgt gctaattcgt ctggcgacgg gtcagggtgt
     3301 cgtttcggcg atgaccgccg ccgggattat cagtgctgca atccttgatc cagcaaccgg
     3361 tcagctggtt ggcgtgaatc cggcgctgct ggtactggcg acggctgcgg gttccaacac
     3421 cctcacccac attaatgatg catctttctg gttgttcaaa ggttactttg acctgtcagt
     3481 aaaagacacg ttgaaaacct ggggtctgct ggagctggtc aactccgtgg ttgggctgat
     3541 tattgtgctg attattagca tggtagcgta aaagaacacc agagcctgcc aatggcaggc
     3601 tcagactgat gactaaagtc taaaaatgtg cccggacagt cccctcgccc ctccggggag
     3661 agggttaggg tgaggggaac agaccagtaa agtgcgcttt gtttatgcca gatgcgacgc
     3721 tgacgcgtct tatctggcct acaaagggct aacgtgcagg ttttgtaggt ctgatagcgt
     3781 agcgcctcag gtaccagcat tagcaatgcg ctgtaatttt tttattgata aacaaaagtc
     3841 acgccaataa tcgattgcac attccctgca gtcacctgcc ctccggtacg tgcgtaattt
     3901 gccgttaatc ccagacttac cgccgaagtt cctactgctc ctaacgatac cgtgttattc
     3961 gttggaataa tcgtaccgtt gcgcgtcaac tgtacgccga cgccctgcgc tggtgaaaac
     4021 gacgcggtat tggtgaaaat cgagttgccc gcatctgcgg ttgtgccgga gaggtaatac
     4081 cccaggtttt ggcttttcgc acaataaacg gtaagaggaa ttggcactga accagggtag
     4141 tccggcagag taacggtgac atcacgagca gaaacatcac aaccgccagt gggcaccacc
     4201 acatcattat tggcgtaaat attccacaca aactgaaaat catcgctgtt atagttgttg
     4261 gtctgtcgca aaataagcac ggcaattaat gaaccagctt taatcgccac cccgcccgca
     4321 ctgctcacag gcgtcaaata aagcgccacc ggccacggct tatccgttct cgaattataa
     4381 acaacccgcg gcgtttcgct ggtggtcgga aatggatagc tactgccact atattttacg
     4441 gtcccggaaa aattagataa cacgccgccg taagccgagc ctcgttgcag tgtgacatag
     4501 tctgtaatgg tttccggata atcgttatgg caaaagattt gcgtcgaaag atctacgacc
     4561 aggttttgcc ccacattcac gacaggcgca aggtttacat aaacattagc gctgccaccg
     4621 ccaataggga tagcggtacc attggcggtt ttacaggcga atgaccaggc atttaccgac
     4681 cagcccatca gcagtacagc aaacagggta atagctcgtt tcattacaat tatctcttcg
     4741 ggttcagctg taggtatagg tgatgctaat cactgcctga atggttccct gagtggctcc
     4801 gccatttacc gtcaatgctc tgacctgtaa cgggaagtgc gctgattgtg aggaatcatc
     4861 cacctgaact gttttggttg cgccagtatt caatgtgttg ccactgtcat cctgtagctc
     4921 taactggatg ttttgcgcgg tcccctggtt tttataatat ccggtgctgt cggctgcccc
     4981 gctgaagctg gcagtgaccc tcgacgttcc caccggacaa ttagtcaact caagcgcaac
     5041 atcatgccag gccgatgccg ccccggcaga cataagactg aaagaataaa gatcgccgag
     5101 atctaccgtg gcattggtgg tggaaaccgt acacggtttg gcgacgacct taccgttcac
     5161 cgtgatggtg acatcggctg cctgtatcgt cgcacttgcg agcgccaaca ttgccgccaa
     5221 tacataccca cgtttgcacc atttcatgag catctccagt tactgatatt caagagtgaa
     5281 ggtagccgta gcattgatat gccccgcagt gacaggcacc tgtgtcgcca ttagccgggc
     5341 gtaaaaattc agcgtatttg gtttacccgg cgtcagggtc gtccacgaaa tcgcagacga
     5401 tggagcatta aggggtattt gattttgctg ctcattcaga agctgtattc ccagtcccgc
     5461 agccgctgac accgtatttt caagtgcaag caggttggca ttgtggctat ctgcaacgcc
     5521 ggtaaaccca acttttacgg cagaaacagg gtgacagcaa aatacgaaat ggaacgacag
     5581 gagtcgtcgc gccaatgttg ttaaattgct tcgccgcgtt ttccatcaga tcaacagtaa
     5641 aattggttga ttcagcggcc acactacagc cgttatctct gacatagccg cggatagtaa
     5701 tcgtgctatc cgcagccaaa acgcgactta ccgccagcca caaaaaagcg cacagaagat
     5761 aaaaaggttt gtttctcatc acgccccctt aacgacattc agctgatagc tgggttaata
     5821 actgctgctg actctctggt ggcagttgat aattggcgac acagtgagca ttttcctctt
     5881 ctccccattt cacctgaact tttcccgcta aaggcattcc gctgaggtaa aactgaccat
     5941 tatccgcaac aatgccgcta ctctggctac tctctgatgt caccatcgcc ccaaacggca
     6001 gcggcttatt attgtgagtt agcgtcatga gcagttttat cccaacgcgc gctttaaact
     6061 ctgctcgcac gatcgcccca cgagtgggaa caacgttagc gaccgcgtta tctaaatcga
     6121 cgttatcagc caaggtattg gtatccagcg ccactctatt ttcccgatat tcagtggcat
     6181 aaggcagcac ggcataacca cgccagtcgg tacgcacccc cgtctggttt tcgacttttg
     6241 catcttttgc gccaggcgct ttaacaagca ccaccgtatc gtttaacggc tgccccagcg
     6301 ttacgccatt ggcatgagcc aataccccac cgctgactcc gtaatagagc tgcttaatat
     6361 catcgctatg gctgtaaccg atattggcat tgccgtaacc accgcgataa ttcagcgtgg
     6421 cgtagcctgt acttccgcta ttaccatcgc ctcccccggc atagccggtt tgcacgctat
     6481 aactgaggtt gttgtcttcc agcaacgtac cgtatacacc agccagattg gtcatccgac
     6541 cgttgagatc gtgtgacatg ctgtagctgg cactggcatg tcgccactga gatttactgt
     6601 cagaacgcag ccagtggctg aaaggaatat tgacgttaag cgctaacatc tgatcccgtc
     6661 ctttttgcca ggcgtttttc gtcaggctat agctgagcgt ccagttgata tcttcgaacg
     6721 cagtatttaa tccagcctgg aattgctcat cgacattact cgttccccaa taagtttgat
     6781 ggctaccact caaatacagt gttgatgtgc gcccgagttg ctgagtaacg gtgagctgta
     6841 atttcccgcg tttgttataa gcgaggttgt aatagtcggt gaatttcggc ttaacctgaa
     6901 taactccatc ctgtgtttcg atgttgtagc cattcattcg actgtatgtt gtatcagcga
     6961 aattaaaata tccgctggtc gaataacggt aacccactaa ctgaatattc gtgcctgatt
     7021 cattgagcga tttgttatag agaaaacgca ccgattgtcc gtcatgctga ctgtcatcgg
     7081 gaagtgtgga attagcctgc gtcatatcca cagacagagc gcccagtgcc tccatgtttt
     7141 tcccgatacc gaaattaaaa gcacgataac gatccgccag ttgcgttcca ccatatattg
     7201 tccagccagc cggaaggccg tggagtaatg tactctggaa aaagcggggt ttttcctgct
     7261 gcgcatttcc actacggtat tctcctgcca taatggaata acgagtatgc ccttcacgtt
     7321 gcaaaagcgg gactgacgaa tagggtacgg taaaaatctg cgtgctgccg tcagcctctt
     7381 tgatcgttac ctgcaagtca ccactattac ctgcggcata gatatcgttg atggtaaaag
     7441 gccccggtgg caccgtacta ttataaatgc catatccatt ttgtttaata gtgacctgtg
     7501 cagtaccatg agcaataccg tggatcaccg gggcaaatcc tctttggcta tcgggtaaca
     7561 tattgtcatc tgaggccaat tgtgcgccgc gaaagttaat accatcgaaa atatcgccct
     7621 gggtataacc atcacccaac gtcagccggg aacgtaaagg aattatgtct cgctcaatcc
     7681 aggtaatgat atgctgccat ttatttttgc tacctgatga actgtcgcta ctgttataac
     7741 tccaggtggt attgtcgcgt aaacgccacg caccaatatt taacccactc tgtaggttta
     7801 aatatgcata atggctgtta cccccaatcc gattctgtac actatttccg ctgaaattat
     7861 aattgagcaa tccggcatta ataccgggat cccataactc aggaggaata taaccacgcg
     7921 cgcgattact cataaatgcc tgagggatcg tcaggttcag tcgctgctga ccaacatcca
     7981 gatgcgcagt agcgtcctgg accattgtgg ttaatggcac acaggcatca tccgccagca
     8041 gattcatacc ggcgacagaa gccgtattca gccccatact ggcgagttgc gcgcgtgtca
     8101 ggcagggaac aatcccttgt tcactgtcgc ccgtattaaa tgtgacatca cgcgttgcca
     8161 tataaccatt attcaaatag atatcgacgc gatacgtccc tggcggtaat tcttgcccat
     8221 tttcaaaacg cgataaatcg gccacagcct ggggatcatc cgctaaaaag cgcagattaa
     8281 aatagaggtc ggcagatgac aaaggtgcct gtgcggcaaa agcacaggcg acaacgagtc
     8341 ggacaaaaaa accagccaaa cgatgcttac gaatatgcaa gcattgtgtg tttcgctggt
     8401 aaagtcttaa attcagatat gacattattc ctccggttta aaatcacccg gcagtcaatt
     8461 ctttttattc aggcgaaaaa ttccccctgc gttattccat tacgcccgtc attttggggg
     8521 taagtgcgcc ataatcattt attgttcggt aagtaatatt gcttcctgca tcagaaggca
     8581 atttaaccgt gctttcgccc attggaggca ccaatgcatt ttcaagaacc cgggttccgg
     8641 cattcaactc
//
LOCUS       DADMGC010000151         8648 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.151, whole genome shotgun sequence.
ACCESSION   DADMGC010000151 DADMGC010000000
VERSION     DADMGC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.151"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..323)
                     /locus_tag="JNZ73_003564"
     CDS             complement(<1..323)
                     /locus_tag="JNZ73_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY8733699.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVALLWVNGE
                     NFRTL"
     gene            complement(333..881)
                     /locus_tag="JNZ73_003565"
     CDS             complement(333..881)
                     /locus_tag="JNZ73_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAY8733700.1"
                     /translation="MGLPPLSKTPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYTEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(881..1588)
                     /locus_tag="JNZ73_003566"
     CDS             complement(881..1588)
                     /locus_tag="JNZ73_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY8733701.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1603..2280)
                     /gene="ydjY"
                     /locus_tag="JNZ73_003567"
     CDS             complement(1603..2280)
                     /gene="ydjY"
                     /locus_tag="JNZ73_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HAY8733702.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2285..2995)
                     /locus_tag="JNZ73_003568"
     CDS             complement(2285..2995)
                     /locus_tag="JNZ73_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY8733703.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYNLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3162..3968)
                     /gene="xthA"
                     /locus_tag="JNZ73_003569"
     CDS             complement(3162..3968)
                     /gene="xthA"
                     /locus_tag="JNZ73_003569"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAY8733704.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGY
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            4414..5634
                     /gene="astC"
                     /locus_tag="JNZ73_003570"
                     /pseudo
     CDS             4414..5634
                     /gene="astC"
                     /locus_tag="JNZ73_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            5631..7364
                     /gene="astA"
                     /locus_tag="JNZ73_003571"
     CDS             5631..7364
                     /gene="astA"
                     /locus_tag="JNZ73_003571"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HAY8733705.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAAGWLAAGRETGQALSALEDLDGLLFTGSANTGYQLHRQLSGQPEKILALEMGGNNP
                     LIIDEVADIDAAVHLTIQSAFVTAGQRCTCARRLLLKSGAQGDAFLARLVAVSQRLTP
                     GNWDDEPQPFIGGLISEQAAQQVVTAWQQLEAMGGRTLLAPRLLQAGTSLLTPGIIEM
                     TGVGGVPDEEVFGPLLRVWRYDTFDEAIRMANNTRFGLFCGLVSPEREKFDQLLLEAR
                     AGIVNWNKPLTGAASTAPFGGIGASGNHRPSAWYAADYCAWPMASLESDSLTLPATLN
                     PGLDFSDEVVR"
     gene            7361..>8648
                     /gene="astB"
                     /locus_tag="JNZ73_003572"
     CDS             7361..>8648
                     /gene="astB"
                     /locus_tag="JNZ73_003572"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HAY8733706.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMLLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQSVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQ"
BASE COUNT         2021 a         2221 c         2370 g         2036 t
ORIGIN      
        1 tttaaggtgc ggaagttttc gccgttcacc cagagcagag ccaccgagcc gcccgtttta
       61 cgaccggcag cagcttcggt ctgaatgcgc ttcaccgcgt ctgcggcatc cgcgagacga
      121 acaatcttca ggtttatagc gtaatgggtt ttcatctcgc cgctaaccca gtcgagatag
      181 cggttaattg cggtatcgcc gccccaggcg ttaaaccaga cggtttgccc tttggcctca
      241 tttttaattt cctgccagtc actggcatgt gttgccaaag aaaacagcga taacaatccc
      301 agcaaccacc cacaatggcg catcttccct ccttaagaac gtttttccgg aatacggcac
      361 agcccctgag cgggaatgtc catggcagaa ttaaaacgag ccgacaggtt ttgcaggccg
      421 atcaatgccg tcagttcggt gagcgcctga gcgtcaaaat gcgcagccag tcgggtacgc
      481 agggcatcat cgactgttgg cggtgtgacg cttgcggctt cggtgtactc cagcgccagc
      541 cgttcttcat cgctaaagag cgggctttgc cgccagtcag ccactgccag cagtttatcg
      601 gtgctgccgg tacgctcggc gactttcata ctggtgatat ccacgcaaaa ctcacacagg
      661 cacatttgcg caatgcgcgc gctgacaagc gatcgcacta ccggatcgag cggtgagcgt
      721 ttgcgctcca gccacccaac aaacattgac accaaataaa agataaacgg gatccgcccc
      781 caccagcgaa tcgggcttag cacctcgccg taatggcgac gatgcagcca cgcctgcgga
      841 cgtaaaatga aaggagtttt gctaagcggc ggtaaaccca tcagtcattc ctcttctggc
      901 gctcaagcca tattttcttc gccataaaaa tcaccacggt cagggcaaac agaataaaca
      961 gttcggtgac aaaccagaaa gtaccgcctg ttaacatgct gcccgcccag gaataaacaa
     1021 tagtcgccgg taattgacca agcccggtgg cgataaaaaa cgagcgaaaa cgtattgaag
     1081 tcagaccggc agcatagctg attggatcga aagggacaaa aggcaataac cgacagacca
     1141 ggatggtgtg tttgccgtag cgagtgaaaa agccgtccat actgtcaagc acggttttgc
     1201 cggttaattt ttccaccact tcgcggccca tcactctggc gataaaaaag cacagcgccg
     1261 cgccggccat cgaactggtc cacgacagca agccgcccca gaacgcgcca aacagcgacg
     1321 cattggcaaa ggtgatcaaa aacgcaggta gcggtgcagc aatggcctgt aaaatcatca
     1381 acaagaacga gacaactgcc gccagtgcgc cgtaagactg aataaagcgt tctataccct
     1441 gttggtccac ggcggcaaac gccgcaacgc tgcgattgat aaactcatgg acgccgggaa
     1501 gcagcgccca tgcgagcagc atagcgaaca acaggatgat gagggttata cggtagtacc
     1561 agattttacg cgattgtatc ttcatcatcc tttatcctgg ctttattcgg tgattttaaa
     1621 ggtaaccgtt gccaacgtgt tatccgccgg gagaactgag gcattgcctt tgaatttcac
     1681 ttcgccgcgt ttttccaccg caccataagt gtatgttgca ttgctgacga tgccgaccgg
     1741 gcagctatcc agacacacca ggcaacccgt tttcttctct tctgctgccg ttaaattacc
     1801 gccaaagcgc atgtccagtt tcttgccatt actgtcaaca atcacttcat cgaaggaata
     1861 cgcttttgcc gccccttgcc agttgaccga aatatccagt ttgctgcctg tgacatgagt
     1921 cgtttcttta ttatccatcg tcatgttttc gcccggcgta ccacctgcct ctttcagggc
     1981 ttcataaaat gctttcgggg tcgcataagc cataaacagc gatttatgtc cgttggagcc
     2041 atctttaaac acaataccgt gacgagtgtc gtcggtgagg aaacgaccat taatttgcac
     2101 cagcatggtt accgtatggt tattggcatc gacgcgcagc ggttggctgt tcgaaagtcc
     2161 gtcgtattcc acttttgccg ccgcattctc tttttgatcg cagccgctaa gcccggcaac
     2221 agccagtaag cagagtgcag ccagtccttt tttccatgac actgaataat gttgcgacat
     2281 cgttctatcc ttcatttttt gggtgagtaa gtgggctgcg gcgcgaagca gacagatcca
     2341 catgtttgtg tcgggcgtag agttttgcga gctggatgag aataaacagc gccaggcccg
     2401 ccagacagag ttgtaaaata aagcgcagcg taatgccttc attggcgaga tcgcttgcca
     2461 tcacggtata aataacaata ccgggcaggg tcgtgagtgc cgaaataagg gtataaggcc
     2521 agaaggcgat ggtggttaat ccgtaagcgt aattttgaat attgtaagga aacaacggga
     2581 ttaagcgggt cagaataaga aaatcaatac cgttacgcgc aatgcctttt tcaatggcct
     2641 ggaaggtatg gctatggccc acgtatttca gcagtaaatc acgtcccagc cagcgcgcca
     2701 gcaggaatga gcacgaggag gccagcgttg cggcaattaa tgagagtagt gtccctaaga
     2761 gcgggccaaa aactattcct ccagcgatca ccagtatgct tcctggtaac agaaagaggg
     2821 gggcattgat gaataacaga atatagaggt tataaccgaa caatccgctc tggcgaatga
     2881 gtgtctgtaa gtggggtaaa tcggtgagca gatcgaataa accgaaagcg tggatagcgt
     2941 aaatgaccag cgcaaaaata agacaggcaa acagaaagtt acgctcagcg ttcatcgtgg
     3001 agtttcctat atcaaacttg ataatagaga agtggtcaga caactaaaaa cgcagtgacg
     3061 gtataacaaa ggacggcagg caacaaatca ttactgacag tttgttgatt tttctcaagg
     3121 ttaattctcc tgacccagtt tgagccagga gagctgctaa attagcggcg gaaggtcgcc
     3181 cagacggggg cgtgatcgga cggtttttcc atgctgcgaa tttcatagtc gatgccggtt
     3241 tctacgcaac attctgccag cggttggctg gcgagcagca ggtcgatgcg cagaccacgg
     3301 ttatcgtcaa aaccttttga gcggtaatca aaccatgaga aacgatctgc tgtttgcgga
     3361 ttagcatggc ggaaggtatc gaccaccccc cagctcatca gcctgtccat ccattcgcgc
     3421 tcttccggca ggaaagagca tttaccggta cgcagccagc gcttacggtt ttcttcgcca
     3481 atgccgatat ccagatctgt agggctgata ttcatatcgc ccataatcag taccggatta
     3541 tcacgtttga gttcggtttc caggtagttt tgcagattct gataaaactg cgcttttgcc
     3601 gggaatttta tcggatggtc gcggctttca ccctgcggga agtaaccgtt gatcacggtg
     3661 acataaccca gcagtgaggg gatttccgcc ataataatcc gccgctgcgc ctcttcgtcg
     3721 tcaccgggaa agccgcgacg cacggcaatc ggcgtctctt tggtcagcag cgccacgcca
     3781 taatggcctt tctgcccgtg ataaaacacg ttgtagccga gcttcgccac ctcttcgagc
     3841 ggaaacatat cgtcatgaac ttttgtctcc tgcaggccaa tcacatccgg ttggtgcttt
     3901 tcgacgatgg cttcaagctg gtgaggtctg gcgcgcaggc cgttgatatt aaaagagaca
     3961 aatttcatag tcgctgccat ttattcagat aaagagtgcg tggatggtag cagaatttcg
     4021 cgttgcttac cgcctgttgt taagtgattt gatcgatggt tgttaatgat gtcaacgatg
     4081 gcgcaaaaaa tgcccgcttt tggtgcgcgc tgcgtcagaa tggcgcagta atttccagta
     4141 aatttcgtca atgatcacaa ttccagaatt atattttact tctgcataac attgcgtttt
     4201 ttattctttt tattcaccca catcgcaaac gtattcactt tatatgcact ttaaatgcat
     4261 atggtttggt tataactcct tgatttccag ttagcctccg ccgtttatgc acttttatca
     4321 ctggctgaca cgaaccctgc aatctacatt tacagcgcaa acattactta ttgttaacat
     4381 acaaataacg aattatttac tgtagaggtc gctatgtctc agccaattac gcgtgaaaac
     4441 tttgatgaat ggatgatacc tgtttacgct ccggcaccct ttataccggt acgtggcgaa
     4501 ggttcgcgct tgtgggatca gcaggggaaa gagtatatcg acttcgcggg tagcattgcg
     4561 gtgaacgcgc tgggccatgc gcatccggaa ctgcgtgaag cgctgaacga acaggcgagt
     4621 aagttctggc ataccggcaa cggttacacc aacgagccgg tactgcgact ggcgaaaaaa
     4681 ttgatcgacg ccacgtttgc cgatcgcgtc ttcttttgta actccggtgc ggaagccaac
     4741 gaagcggcgc taaaactggc gcgtaaattc gctcacgacc gctacggcag ccataagagt
     4801 ggcatcgtgg cgttcaaaaa tgcgtttcat ggtcgcacgc tgtttactgt cagtgcgggt
     4861 gggcagccag cctattcaca ggattttgcg ccactgccgg cggatattcg tcatgctgca
     4921 tataacgata ttaactctgc cagcgcgctg attgacgacg ccacctgtgc ggtgattgtc
     4981 gagcccatct agggcgaagg cggtgtggtg ccagccagca acgcgttttt acaaggtctg
     5041 cgtgaattgt gtgaccgcca caatgccttg ctgatttttg atgaagtaca aaccggcgtc
     5101 gggcgcactg gtgagctgta tgcctatatg cactacggcg tgacgcccga tctgttaacc
     5161 accgccaaag cgctgggcgg cggtttcccg gtcggtgcgt tgttggcaac cgaagagtgc
     5221 gcccgcgtga tgaccgttgg cactcatggc accacctatg gcggtaatcc gctggcctcg
     5281 gcggtagcag gcaaagtgct ggagctcatc aacacaccag agatgcttaa tggcgttaaa
     5341 cagcgtcacg actggtttgt tgagcgtctt aatactatta atcaccgcta tggtttgttc
     5401 agtgaagttc gcggcttagg tttgttgatt ggctgtgtac tgaatgccga ttacgccggg
     5461 caagcgaaac agatctctct ggaagcggcg aaagcaggcg tgatggtact gattgcgggt
     5521 ggcaacgtgg tgcgttttgc gcctgcgctc aatgtcagcg aagaagaggt gacgaccgga
     5581 ctggatcgct ttgcagctgc ttgcgaacac tttgttagcc gaggttcatc atgatggtca
     5641 tccgtcccgt tgagcgctca gatgtctcgg cgctgatgca gcttgccagc aaaacgggcg
     5701 gcggcctgac gtcgcttccc gccaatgaag ccacgctttc ggcgcgtatc gaaagggcaa
     5761 tcaaaacctg gcaaggcgaa ctgcctaaaa gcgagcaggg ctatgtgttc gtgctggaag
     5821 atagcgagac aggcaccgtg gcggggattt gcgctattga ggtggcggtt gggctgaacg
     5881 atccctggta caactatcgc gtcggcacgt tggttcacgc ctcaaaagag ctgaatgtct
     5941 ataacgcatt gccgacgctg tttcttagta acgatcacac cggcagcagc gagctgtgca
     6001 cgctgtttct cgacccggac tggcgcaaag agggcaacgg ctatttgctg tcgaaatcgc
     6061 gctttatgtt tatggcggct tttcgcgaca agtttaatga caaagtggtt gctgaaatgc
     6121 gcggggtgat tgacgaacac ggctattcac cgttctggca aagcctcggt aaacgcttct
     6181 tttcgatgga ttttagccgc gccgattttc tctgcggcac cgggcaaaag gcatttattg
     6241 cagaactgat gccgaaacat ccgatctata cccacttttt atcccaggaa gcccaggacg
     6301 tcatcggtca ggtacatccg caaaccgcgc ctgcccgcgc ggtgctggag aaagaaggtt
     6361 ttcgttaccg caactatatc gacatcttcg acggcgggcc aacgcttgag tgtgacatcg
     6421 accgcgtgcg cgccatccgt aaaagtcggc tggtggaagt ggcagcaggc tggcttgccg
     6481 ccgggcgtga aacgggtcag gcgctgagtg cgctggagga tctcgacggt ttgctgttta
     6541 ccggtagcgc caataccggc taccagctgc atcgccagct ctccggtcag ccggagaaaa
     6601 ttctcgccct tgagatgggc ggtaataatc cgctaattat cgatgaggtg gcggatatcg
     6661 acgcggctgt ccatctgacc attcagtcgg cgtttgtcac agccgggcaa cgctgcacct
     6721 gcgcccgccg tttattgctc aaaagcggag cgcagggcga tgcgtttctt gctcgtctgg
     6781 ttgccgtcag ccagcgatta acgccgggca actgggatga cgaaccgcag ccgtttattg
     6841 gcgggctgat ttctgaacag gccgcacagc aggtggttac tgcctggcag caactggaag
     6901 cgatgggcgg acgaaccctg cttgcgccgc gcttattaca agcagggaca tcgttgctga
     6961 caccagggat cattgaaatg acgggcgttg gcggtgtgcc ggatgaagag gtgttcgggc
     7021 cgcttttgcg cgtctggcgt tatgatactt ttgatgaggc gattcggatg gcgaacaaca
     7081 ctcgcttcgg actcttttgc ggtctggttt cccccgagcg ggaaaaattc gatcaactgt
     7141 tgctggaggc gcgggcgggg attgttaact ggaacaaacc gcttactggt gctgccagta
     7201 ccgcgccatt cggcggcatt ggtgcttccg gtaaccatcg ccccagcgcc tggtatgccg
     7261 cagattactg cgcatggcct atggcgagcc tggagtcgga ctcgttaaca ttgcccgcca
     7321 cgcttaaccc cgggctggat ttttccgatg aggtggtgcg atgaacgcct gggaagttaa
     7381 tttcgacggg ctggtagggc tgacgcatca ttacgcgggc ctgtcgtttg gtaatgaagc
     7441 ctctacccgt caccgttttc aggtgtctaa cccgcgactg gcggcaaaac agggcttact
     7501 gaaaatgaaa gcccttgccg atgcgggatt cccccaggcc gtgatcccgc cgcacgagcg
     7561 tccgtttatt ccggtgctgc gtcagttggg attcagtggt agcgatgagc aggtactgga
     7621 aaaagttgca cgccaggcac cgcactggct ttccagcgtc agttccgctt cgccaatgtg
     7681 ggtagccaat gcggcaacga tcgcgccatc tgccgatacg ctggatggca aagtgcatct
     7741 caccgttgcc aacctgaaca ataaatttca ccgttcgctg gaagcgcccg tcactgaatc
     7801 gctgttaaaa gcgattttta aggacgaaga gaaatttagc gtccattcgg cgttgccgca
     7861 ggtagcgttg ctcggtgatg agggggcggc aaaccacaat cgtctcggcg gtcattacgg
     7921 tgaaccgggt atgctacttt ttgtctacgg gcgagaagaa ggcaatgata cccggccttc
     7981 ccgttatccg gcgcgacaga ctcgcgaagc cagcgaggcg gtggcaaggc ttaatcaggt
     8041 gaatccccaa caggtgattt tcgcccagca aaacccggac gttatcgacc agagcgtttt
     8101 tcataatgac gtgattgccg tgagtaaccg ccaggtgctg ttttgccacc aacaggcgtt
     8161 cgctcgccag tcacagttac tggcaaacct gcgggcgcgg gtgaacggtt ttatggcgat
     8221 agaagttccg gcaactcagg tttccgtgtc ggatgcggtg tctacctatc tgtttaacag
     8281 ccaactgttg agccgcgatg atggttccat gatgttggtg ctgcctcagg agtgtcggga
     8341 acacgccgga gtatggggtt atctcaatga actccttgcc gctgacaacc cgattagcga
     8401 actaaaagtc tttgacttac gtgaaagcat ggcgaatggc ggcggcccgg cgtgcctgcg
     8461 gttgcgggtg gtattgacag aagaagaacg ccgggcggtg aatccggcgg tgatgatgaa
     8521 cgataccttg tttaatgcgc tcaatgactg ggtggatcgt tactaccgcg atcgtcttac
     8581 tgctgccgat ctggccgacc cgcaattgct gcgcgaaggg cgggaagcac tggatgtatt
     8641 gagccaat
//
LOCUS       DADMGC010000152         8496 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.152, whole genome shotgun sequence.
ACCESSION   DADMGC010000152 DADMGC010000000
VERSION     DADMGC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.152"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..920)
                     /gene="rspA"
                     /locus_tag="JNZ73_003573"
     CDS             complement(<1..920)
                     /gene="rspA"
                     /locus_tag="JNZ73_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HAY8733707.1"
                     /translation="MHCERKTYQAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRE
                     LSVASYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKA
                     KAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMK
                     TTYGMSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMH
                     HRLTPIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDC
                     KQLIEEQLIDYIRTTLTHAGGITGMRRIAD"
     gene            962..1303
                     /locus_tag="JNZ73_003574"
     CDS             962..1303
                     /locus_tag="JNZ73_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HAY8733708.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1369..1898
                     /gene="speG"
                     /locus_tag="JNZ73_003575"
                     /pseudo
     CDS             1369..1898
                     /gene="speG"
                     /locus_tag="JNZ73_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(1901..2611)
                     /locus_tag="JNZ73_003576"
     CDS             complement(1901..2611)
                     /locus_tag="JNZ73_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HAY8733709.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            2719..3024
                     /locus_tag="JNZ73_003577"
     CDS             2719..3024
                     /locus_tag="JNZ73_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HAY8733710.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            3223..4344
                     /locus_tag="JNZ73_003578"
     CDS             3223..4344
                     /locus_tag="JNZ73_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY8733711.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYP
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTTKPAYIC"
     gene            4378..4701
                     /locus_tag="JNZ73_003579"
     CDS             4378..4701
                     /locus_tag="JNZ73_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY8733712.1"
                     /translation="MTARAIAMLPILTGNVGISGGNSGARESTYTITIERLPVLDNPV
                     KTSISCFSWTDAIDHGPQMTALRDGVRGKDKLDVPIKFIWNYKDLKRLLVRVTRDLSR
                     PEVLH"
     gene            complement(5293..6282)
                     /locus_tag="JNZ73_003580"
     CDS             complement(5293..6282)
                     /locus_tag="JNZ73_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733713.1"
                     /translation="MTMIKDSYQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLSDRSVVVPVRGGERPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTHGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTTKADAIMDALFENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTVKLDTGAATAVLPVVKRLKFTKTEA"
     gene            complement(6357..6866)
                     /locus_tag="JNZ73_003581"
     CDS             complement(6357..6866)
                     /locus_tag="JNZ73_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733714.1"
                     /translation="MSSKASALFNNNDWKLHVTDINLYENTVSLDGQSYPLSFAIKTL
                     IPGYLSGLPATSRESMELLEALAEAGVTIGNFFSNDLMTAYQRRQQNKRAEAERIAKE
                     QRIQAERMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADIE
                     SGGNWMDSL"
     gene            complement(6968..7189)
                     /locus_tag="JNZ73_003582"
     CDS             complement(6968..7189)
                     /locus_tag="JNZ73_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733715.1"
                     /translation="MTTKQHIDPRTPIGKATLRYRGLPTRRLLSMLGMGVEDPERPFY
                     SRDELIDLLVDRDLNNQLRRAFAKLDATH"
     gene            complement(7436..8281)
                     /locus_tag="JNZ73_003583"
     CDS             complement(7436..8281)
                     /locus_tag="JNZ73_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733716.1"
                     /translation="MNTNEVSVINLDQLVSMSSYDFLTKVINPARVEAGEKPVRNNDF
                     VSRIEDELDGQNVAYEIFVSFGQDVKSYTLNTDQLLLVGMRESKAVRRKVLAYIRQKE
                     QEVLELQAQQLQVAQTKAICYAPGAIVLDSKLDSKSGATPTEVIKALDLKLTSSKMLT
                     ASVLTGYLKTLCRPNPFYTKRVTEFGTTDIDLSTGRPRAIFNKKGETNGYYRLQPQPN
                     DKEPTIKILRKGYDWLKDNWKDIAHDMRTSNIKVVRRLVDSHLNGKATKSQKAAAGKG
                     SDYGF"
BASE COUNT         2126 a         2133 c         2130 g         2107 t
ORIGIN      
        1 aaatcggcaa tccggcgcat accggtaatt ccgcctgcat gggtcagcgt ggtgcggata
       61 taatcgatga gttgctcttc aatcagttgt ttgcagtccc agatgctgtt gaagacttca
      121 cccactgcga tgggtgtgac ggtatgttgg cgaatgagac ggaagcattc ctggttttcc
      181 gctggcgtcg ggtcttccat ccagaacatg cgataatctt caatgctttt accaaagcgc
      241 gccgcttcaa taggcgttaa gcgatggtgc atgtcatgca gcaaatgttc attaaaacca
      301 aacttgttac gtaccgcgtc aaacaatttc ggcatgaaat cgaggtattt ctccgtcgac
      361 cacagctgct cttccggcca ctgtcctttg gttgcgggtt cataagccag acctttacct
      421 ttcgacatgc cgtaggtggt tttcatacca gggattccgc actgcacgcg gatggctttg
      481 aatcccagct cctgatgacg ggcataatca tccagagctt catcaatact gtgaccggtg
      541 gtatggcaat aaaccatcac cccttcacga gacgcgccgc cgagtaactg gtaaagcggc
      601 atgttggcag ctttggcttt aatatcccac agcgccatat caaccgctga aatggccgac
      661 atcgtaaccg gaccgcgacg ccagtaagca cctttataga aaaactgcca gatatcttcg
      721 atacggtgcg catcgcgacc aataagccgc ggacaaagat gatcctgcaa ataagaggcc
      781 acggaaagct cacgtccatt gagggtggca tccccaaggc ccgtaatacc gtcctcagtg
      841 gtgattttta atgtgacgaa attacgcccc ggacaggtaa caaaaacttc agcctggtac
      901 gttttgcgtt cgcagtggat ggtgtagaat cacctttaat cattcataca gggaatgaat
      961 tatgaaaatc actctcagca aacgaatcgg cctgctcgct tttctgctgc cttgcgcact
     1021 ggcattgagc acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag
     1081 tgcacaaagc cgccagcacg ccgctatgga aaaagagcaa tggaatgaca cgcgcaatct
     1141 gcgccagaaa gtgaataaac gcactgaaaa agagtgggat aaagccgacg ccgcttttga
     1201 taaccgcgat aaatgtgagc aaagcgccaa catcaatgcc tactgggagc ccaatacttt
     1261 gcgctgcctg gaccgtcgaa ctggccgcgt tattaccccc taacctgtta ttgatttaag
     1321 gaatgtaagg acacgttatg ccaagcgccc acagttaagc tacgcccgct ggagcgtgaa
     1381 gatttacgct atgtacatca actcgacaat aacgccagtg tgatgcgtta ctggtttgag
     1441 gaaccctacg aagcctttgt tgaactctct gatctgtatg ataagcgcat attcacgatc
     1501 agagcgaacg gcgctttgtg gtggaatgtg acggcgaaaa agccggtctg gtggagctgg
     1561 tggaaattaa ccatgttcat cgccgggcag aatttcagat aattatctcc ccggagtatc
     1621 aggggaaagg tctggcaacc cgtgccgcca aattagcaat ggactatggc ttcaccgttc
     1681 tcaatctcta taagctgtat ctgatcgtcg ataaagagaa tgaaaaagcg attcacattt
     1741 accgcaagct tggctttacg gttgaaggtg aattgatgca tgagtttttt attaatggtc
     1801 aatatcgtaa tgccattcgc atgtgtatat tccagcatca gtatctggca gagcacaaaa
     1861 caccgggtca gactctcctg aagccgaccg cacaatagca ttaataataa tcgatcgtat
     1921 ttttgatggt gtaaacccgt tcgacggcgg gttttactcc ttcatcaaca atgatcagct
     1981 gacagtccac cggattggcg tgactgtcat attcacagct ctgttttaca ttaccaactc
     2041 gttgattatt tagtaaagta accgctgtgt aatctaattt tttgatcgga tccgttgatg
     2101 gcgtggcgct gacagataat gttttgtcgt tacttttcgt ggttttaccc agcggatacc
     2161 cctgatcatc atagcgatat tccatctgca tttgtttgct gctggcttta atcacgaagc
     2221 cattatcatc ggtttcccaa ctcaccccgg cagaaggtaa ttctgctagc tggcattttc
     2281 cctgtaaacg tactctcttc tccagcgtct cggcatcacg gtaataattg gcgtccagta
     2341 ccagagcgac cacggtatta ttttccagat ccagtaattc gagtgaatca aaacagcctt
     2401 cttccgacaa agtcccagaa acacgtttcg tcacttcacc ttgctcatcc attaatgtct
     2461 gagtgaaatc ttttaccgga ccacgcagcg gatcaaaatc gaattcatta gagaaactgg
     2521 ccatctcagg ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg aggaatatcg
     2581 cgagtaagca tggtagtaat ttgtatttca caacagtcac cagagagtag agatgattct
     2641 caatcatagt agcaaataca gtactttaca cgttaaatgc tatgcttaaa gaagttatct
     2701 tcgcgtaagg agcttatgat gaaactctca acctgctgtg ccgcgcttct gctcgccctc
     2761 gcctcacccg cggtactcgc cgcgccggga tcctgtgagc gcatacagag cgatatatca
     2821 cagcgcatta tcaataatgg cgtacccgaa agcagcttca cgttaagtat tgtacccaat
     2881 gaccaggttg atcagcctga ttcccaggtc gtcgggcatt gcgctaatga tacgcataaa
     2941 attctctaca accgcaccac cagcggtaac gtctctgctc ccgcgcagtc tacccaggat
     3001 ggcgcgcctg ccgaaccgca gtaacacatt atcgactgaa cgccggatat gacacatccg
     3061 gcgatttgaa catacaacat aatctcacct tattactcat acccttctat tgatgtggat
     3121 taataattct taacccaaaa tgggtagact ccctctattg ttagcgcgct aaatattcaa
     3181 tatataaaca tttatataac gataaagaac agggagtgag ttatgtccaa aaatgaccga
     3241 atggtgggca tcagccgcag aacactcgtt aaatcaaccg cgataggttc tctggcgctg
     3301 gctgcaggcg gtttttcttt accgttcacc ctgcgcagtg cagcggcagc ggtacaacag
     3361 gccagcgaaa aagtgatctg gggttcctgt tccgtcaact gtggtagccg ctgtgcactt
     3421 cgtctacatg ttaaagataa tgaagtgacc tgggtggaaa ctgacaatac cggcagcgat
     3481 gagtacggca accatcaggt acgcgcctgt ttgcgcggtc gctccatccg ccggcgtatt
     3541 aatcatcccg atcgcttgaa ttacccaatg aaacgcgtgg gtaaacgcgg tgaaggcaaa
     3601 ttcgaacgga ttagctggga tgaagccctg gatactatcg ccagtagcct gaaaaaaacc
     3661 gtcgaacagt atggcaatga agctgtatat attcagtacc cttcggggat tgttggcggc
     3721 aatatgaccc gctcttcgcc atcagcctcg gcggtcaaac gcctgatgaa ctgctacggc
     3781 ggttcactca atcagtatgg ctcctacagc accgcgcaaa tttcctgtgc catgccctac
     3841 acctacggca gtaatgatgg caacagcacc acggatattg aaaacagcaa gctggtcgtg
     3901 atgtttggta acaacccggc agaaacccgc atgagcggtg gtggcatcac ttatcttctt
     3961 gaaaaagcgc gcgagaaatc gaacgccaaa atgattgtta tcgatccgcg atataccgat
     4021 acggctgccg gtcgtgaaga cgaatggctc cctattcgcc cgggcaccga tgccgcgctg
     4081 gttgcgggta ttgcctgggt attgattaac gaaaatctcg ttgatcaacc ttttctcgat
     4141 aaatactgcg tcggctatga cgaaaaaacc ttacccgcag atgcacccaa aaatggtcac
     4201 tataaagcct atattcttgg tgaaggtgac gataaaacag caaaaacgcc gcagtgggct
     4261 tcgcaaatta ccggtatccc ggtagaccgt atcatcaaac tggcgcgtga aattggcaca
     4321 acaaaacccg cttatatctg ctagggctgg gggccgcaac gccaggcaaa cggcgagctg
     4381 actgcacgcg ctattgctat gttacctatt ttgacgggca atgtcggcat cagcggcgga
     4441 aatagtggcg cgcgtgaatc gacctatacc attacgatag aacgcctacc ggtgctggat
     4501 aatccggtca aaacgtcaat ctcctgcttc agctggacgg atgctatcga tcatggcccg
     4561 caaatgacgg cactccgcga cggtgtccgg ggcaaagata aactggatgt gcccattaag
     4621 ttcatctgga actacaagga cttgaagcgg ttactcgtta gagtcacgag agaccttagt
     4681 aggcctgagg tcctgcattg agtcaagacc ttgtgtcatt ctttgtgcca gacttgtgtc
     4741 attagacggg atgtctagac ggctggtcag gcccccggtg gcctctttgt caggactcag
     4801 ggtggcaccg gggaacattg gtgtcttaat tgtattgaat gaggtctcta acttttggtc
     4861 caaaactcat cgttagctgc tcgccagctc acagccgaac ccgaactgta agcacgacca
     4921 ccagcaccag cttcagcagg aagacgacaa gatagacttt atgcaggtcc atgcgtcctc
     4981 ctgtgtctat tgtaccactg cactagataa ctagtacaaa ataaagtcca gaccgaaggc
     5041 ccagaccata gttaattaat cacattgata atccaccacc tgaagtcctg ccaacattca
     5101 gtccagcctt acccatgcta ggagtcttaa ccggggtgtc gttctgtgtc tccaccactg
     5161 gtgccgcatc aggctctaca ggaagctcca cagcaggcgc agggaccgga ggtgatacgg
     5221 atgtaccacc accagcggag aagcccttag cgatggaccg gaggccttta ctaatgctct
     5281 tacccattgc tgttacgctt cggtcttggt gaacttcagg cgtttgacta caggcaggac
     5341 tgcggttgca gcgccagtgt ccagcttaac tgtcgtagca gcttcagcga caacgaagac
     5401 cttaccgagg atttccaagt cgtgggactg agagtcgcca tcacgtgaaa ggacgaccgt
     5461 aatattctca ccgtcgtgac ggaagcagcc gaagctcagg gccgtgaagc gtttagccag
     5521 catgaacaca cctttgtcct caccgttgta atacgcgatg tcggtaccca gcatatccac
     5581 gatagcgtct tcaccagaga agccctgttt agcagccaga cgggacagcg ctttcagttg
     5641 ggactcatgg gcgatgatgc cgtaatcaga cacttcagag ccgaaagagg actcggtgtt
     5701 ctcaaacaga gcgtccatga tagcgtctgc cttagtggtc aggcttccag tcaggactgg
     5761 cagctctacg gtcttcagca gcttggagga ctccagaacg tcaaccatgc gagcgttgat
     5821 ggtacggacc acattctcac agatgttcat agcgtcctgc tggagtgtgc catgagtgaa
     5881 accagtggag gacttggcgt cccacagttt agtttgctcc gagatgagga ttgcctgttc
     5941 gataggttcg acgacaatct cctgaccaga cggaacggtc acgacttcag gtcgctcacc
     6001 accgcgtaca gggacgacca cagagcggtc agacagggtg tgggcgatga ttggggtagg
     6061 caggctattg cgggtaggca ggacttctac agggaacgga atgttatcct gagcgtcttt
     6121 aagtacaccg ttcagcatag ccttaaccgt cttagctaac tggagggcat agacctgtgg
     6181 gtcagcgtta gttggcagac cttcgttaac ctgaatgttg tcggtgtcgg tgatacgtac
     6241 tggctcaatg ccgaactgat agctatcttt aatcatggtc ataataatca atctcctaaa
     6301 ttagttttag atactgtggg tcccgacatc aggtcaggac cacttgtgtg tctgtcttag
     6361 aggctgtcca tccagttacc gcctgactca atgtcagcga caatagcggc acgggagcga
     6421 ccagcggaat gggtagcact gcggaggttc tcaccatagg tccggcgctc agctttgacc
     6481 tgttcgcggc gctgtagctc tttctgccac tctgcatcgg tcatgttctc ttcacgcata
     6541 cgctcagcct gaatgcgctg ctctttagcg atacgctcag cctcggctcg cttgttctgc
     6601 tggcgacgtt ggtaggcggt catcaggtca ttgctgaaga agtttccaat ggtcacgcca
     6661 gcttcagcca gagcttccag taattccatg gactcgcggc tggtggcagg cagtcctgac
     6721 aggtagcccg gaatcagggt cttaatagcg aaggacagtg ggtaggactg accgtccaga
     6781 ctcacagtgt tttcgtagag attgatgtcg gtgacatgga gtttccagtc attgttgttg
     6841 aataacgcac tagctttgct agacatagta attactcctc ttgagagttg agactatcgg
     6901 taccgcagac acagttatgc ctacgggtct ccccgcatcg gtcatctcaa aaggtttaaa
     6961 ttaggggtta gtgagtggcg tccagcttag cgaacgctcg gcgtaactga ttgttcaggt
     7021 cacggtcgac cagcaggtct atcagttcgt ctcgactata gaaaggacgc tcagggtcct
     7081 ctacgcccat ccccagcatt gacaggaggc gacgggtagg cagaccacgg taacggagag
     7141 tagcctttcc gatgggtgtc cgtgggtcga tatgttgttt agttgtcatt gttagaagtc
     7201 ctcgttagac caatcatcca cgtcaaattc actggtgata ggagcttgga agttaggtag
     7261 accctctggt atctctagag gcagtcgctc gtcgactctc agtgccatct cttcgtgtat
     7321 atgccgaagc agagaagtca gtaagatagg gtccgcagga tgctcagggc cgtgtctctc
     7381 aatgaaacat atggttgtcg ctattgactc cataatcagt tcatcagtga gtcgcttaga
     7441 acccatagtc agaccccttg cccgctgctg ctttctggga ctttgtagcc ttaccattca
     7501 ggtgagaatc caccagacga cggacaacct tgatattgga tgtgcgcatg tcatgagcga
     7561 tatccttcca gttatccttc agccagtcat aacccttgcg tagaatcttg atggtaggct
     7621 ctttgtcgtt aggctgtggc tgtagacgat agtagccgtt ggtctcacct ttcttattga
     7681 agatggctcg tggacgtccg gtcgataggt ctatatccgt agtgccaaac tcagtaacac
     7741 gcttggtgta gaatgggtta ggacgacaca gggtcttcag atatccagtc aggacgctag
     7801 ctgtcagcat cttagagctg gtcaacttaa ggtccagagc tttaataacc tcggtaggcg
     7861 tggcgcctga cttggagtcc agcttggagt cgaggacgat agcaccgggc gcatagcaga
     7921 tagctttggt ctgtgcgacc tgaagttgct gggcctgaag ttcgaggacc tcttgctcct
     7981 tctggcgaat ataggctagg accttacgac gcacagcttt agactcacgc atcccgacca
     8041 gcagaagctg gtcagtgttg agggtatagc ttttaacgtc ctgcccgaag cttacgaaaa
     8101 tttcgtaggc tacattttga ccgtccagct catcttcaat acgggacaca aagtcgttgt
     8161 tacggacagg cttctcccca gcctccacac gagccgggtt gatgacctta gtcaagaaat
     8221 cgtagctact catagacaca agctggtcaa ggttgatgac gctaacttcg ttggtattca
     8281 taaaggatgc tcctttggta aagcactttc tgtctccatg tggagtccgc acctattcaa
     8341 tgcatccaaa cggacccacc aaaaagtgct taacgaaagt gaatagggtt aatgttgatt
     8401 agtgttggtc tggagtgacc atgttctcca tgtgcatata aggcgagacc gcatgtcagg
     8461 acgagctgta aggaccccag atggtgagcg gtgata
//
LOCUS       DADMGC010000153         8349 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.153, whole genome shotgun sequence.
ACCESSION   DADMGC010000153 DADMGC010000000
VERSION     DADMGC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.153"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..157
                     /locus_tag="JNZ73_003584"
     CDS             <1..157
                     /locus_tag="JNZ73_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="traD domain protein"
                     /protein_id="HAY8733717.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(166..564)
                     /gene="vapC"
                     /locus_tag="JNZ73_003585"
     CDS             complement(166..564)
                     /gene="vapC"
                     /locus_tag="JNZ73_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HAY8733718.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(564..791)
                     /gene="vapB"
                     /locus_tag="JNZ73_003586"
     CDS             complement(564..791)
                     /gene="vapB"
                     /locus_tag="JNZ73_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HAY8733719.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            873..1292
                     /locus_tag="JNZ73_003587"
     CDS             873..1292
                     /locus_tag="JNZ73_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005030230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HAY8733720.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTGDDGRTVRNGADR"
     gene            1279..6144
                     /gene="traI"
                     /locus_tag="JNZ73_003588"
     CDS             1279..6144
                     /gene="traI"
                     /locus_tag="JNZ73_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HAY8733721.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQYNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISSEQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQVETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIA
                     PGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQVPR
                     LEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEA
                     REQTLIVTHLNEDRRVLNSMIHDAREKAGELGKVQVMVPVLNTANIRDGELRRLSTWE
                     NNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTIRV
                     GTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLA
                     YAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQAYTDNRQGWTD
                     AINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPT
                     RFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQF
                     VALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIP
                     DNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQA
                     VREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDMVR
                     DLQKEKTPGGD"
     gene            6164..6910
                     /gene="traX"
                     /locus_tag="JNZ73_003589"
     CDS             6164..6910
                     /gene="traX"
                     /locus_tag="JNZ73_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAY8733722.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6965..7525
                     /locus_tag="JNZ73_003590"
     CDS             6965..7525
                     /locus_tag="JNZ73_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAY8733723.1"
                     /translation="MIEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPWLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7656..7868
                     /locus_tag="JNZ73_003591"
     CDS             7656..7868
                     /locus_tag="JNZ73_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HAY8733724.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8052..>8349
                     /locus_tag="JNZ73_003592"
     CDS             8052..>8349
                     /locus_tag="JNZ73_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAY8733725.1"
                     /translation="MLHYSGGLKYRWHLSDMENNMRKYIPLVLFIFSWPVLSADIHGR
                     VVRVLDGDTIEVMDSLKAVRIRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1916 a         2095 c         2673 g         1665 t
ORIGIN      
        1 cagtgaatcc ggtgaagtgg tggatatggc cgcttatgag gcatggcagc aggaaaatca
       61 tccggacacc tggcagcaga tgcagcgtcg tgaagaggtg aacatcaatg tgcaccggga
      121 acgcggggag gatgttgagc cgggagatga tttctgatga acaggtcagc tccagtcttc
      181 agttctcagg ccgcccacac gttcaaattc ccgggtgtta ttagtcacaa taatcagccc
      241 ccgactgcgg gcatgacctg cgatcatttg atcaaatggc ccgacagggc gtccctgacg
      301 ggcaagttct gctcttatct ggccggtgtg tgtggctgca gcagcgtcgt aatccagaac
      361 atcaatgcgg gaaacaaatc cctcgatcac agcgagatta cgttcaggca tctggctttt
      421 ttctgcacca tatatcagct ccatcagggt gaccgaactg atacacattt tcccctggtt
      481 caggttaaaa cgctccctga cgctggcggg tttgttcttt atcgtaaaaa tgcagatgtt
      541 ggtatcgagc ataaacttca gcatcagaat gactcccttt cctgcatgcc gggctgttcc
      601 ctgttatcca taaaatcggt gctgacgctg tgtccgtcga accattcgtc ccatgtctct
      661 ccggctggcg taatgattct ggttcgtccg acagcaatca cttcaacgcg ttttacgttt
      721 tccggcaatg caaccgcttt tggcagtctg accgcctggc tgcggttgct gagaaatacg
      781 gtggtttcca ttatttgcct ccttatgtgg atatgtcttg tgtatatcta ttgtgggatt
      841 gataccggga tatgtcaaag ggatatacgt ttatgatgag tatcgcgcag gtcagatcgg
      901 ccggaagtgc cggtaattat tataccgaca aggataatta ctatgtgctg ggcagcatgg
      961 gagaacgctg ggccggccag ggggctgaac aactggggct gcagggcagt gtcgataagg
     1021 atgtctttac ccgccttctg gagggcaggc tgccggacgg ggcggattta agccgcatgc
     1081 aggatggcag taacaggcat cgtcccggct acgacctgac cttctccgct cccaaaagta
     1141 tctccatgat ggccatgctc gggggggaca aacgtcttat tgaagcacat aaccaggccg
     1201 tggattttgc tgtccgtcat cacccgtgtg gaggcactgg cctccacacg ggtgatgacg
     1261 gacggacagt cagaaacggt gctgaccggt aatctggtga tggcactgtt taaccacgac
     1321 accagtcgcg atcaggaacc acagttacac acgcatgcgg tggtgactaa tgttacgcag
     1381 tataacggcg aatggaagac actgagcagt gacaaagtgg ggaaaacggg gttcattgag
     1441 aatgtgtacg ctaatcagat tgcctttggc aggctctacc gggaaaaact gaaagagcag
     1501 gttgaggcgc tgggctatga aactgaagtg gtcggtaagc acggtatgtg ggaaatgccg
     1561 ggtgtaccgg tggaggcctt ttccggacgc tcacagacta tccgggaggc cgtcggggaa
     1621 gacgcctcgc tgaaatcccg ggatgtggcg gcgctggata cgcgtaaatc caaacagcac
     1681 gtcgatcctg aagtcagaat ggccgagtgg atgcagacgc tgaaggaaac cgggttcgac
     1741 atccgggcat atcgtgacgc agcggaacaa cgggcatata cccgcacgca gacacccgga
     1801 cccgcttcac aggacgggcc ggatgtgcag caggcggtga cacaggccat tgccggatta
     1861 agtgaacgca aagtgcagtt tatgtatacg gacctgctgg ccaggacggt cggcatactg
     1921 ccgccggaaa atggtgtgat tgaacgggca cgcgccggta tcgatgaggc catcagcagt
     1981 gagcagctta tccccctcga tcgtgagaag gggctgttca cgtccgggat tcatatgctc
     2041 gatgagctgt cagtccgggc actcagtcgt gacatcatga aacagaaccg ggtgaccgta
     2101 catccggaga aaagtgtccc ccggacggcc ggttacagcg atgccgtcag tgttctggca
     2161 caggaccgtc cgtcgctggc cattgtgtcc gggcagggcg gtgcagccgg gcagcgtgag
     2221 cgggtggctg aactggtcat gatggcccgg gagcaggggc gggaggtgca gattatcgct
     2281 gctgaccgtc gctcgcagat gaacctgaag caggatgaac ggctgtccgg tgaactgata
     2341 accggacgtc gtcagttgct ggaaggcatg gccttcacgc cgggcagtac cgttatcgtc
     2401 gaccagggcg aaaaactctc cctgaaagag acgttaaccc tgctggatgg tgccgcacgt
     2461 cataacgtac aggtcctgat aaccgacagc gggcagcgaa ccggtacagg cagtgcactg
     2521 atggccatga aggatgccgg ggtgaacaca tatcgctggc aggggggaga acagcgaccg
     2581 gccaccatca tcagtgaacc ggaccgtaat gtccgctatg cccggctggc cggagatttt
     2641 gcggccagcg tgaaagccgg agaagagagc gtggcacagg tcagcggagt acgggaacag
     2701 gccatactga cacaggccat tcgcagtgag ctgaaaacac agggcgtgct cggacacccg
     2761 gaggtaacca tgaccgccct gtcaccggtc tggctggaca gccggagccg ttatctgcgg
     2821 gatatgtacc gcccgggaat ggtgatggag cagtggaacc cggagacacg cagtcatgac
     2881 cgctatgtga cagaacgtgt gacggcgcag agtcacagcc tgaccctgcg gaatgcgcag
     2941 gtcgaaacgc aagtggtgcg tatttcttcc ctggacagca gctggtcgct gttccggccg
     3001 gaaaaaatgc cggtggcaga cggtgagcgt ctgagggtga cagggaaaat tcccggactc
     3061 cgcgtctccg gcggtgaccg cctgcaggtg gcatccgtca gtgaagatgc gatgacggtt
     3121 gttgtgccgg gacgggctga accggccacc ctgcctgtga gcgattcacc gttcacggca
     3181 ctgaagctgg agaacggctg ggtggaaacg cccgggcatt ccgtcagcga cagtgcgacg
     3241 gtttttgcct ccgtcacaca gatggcaatg gacaatgcca ccctgaatgg tctggcccgc
     3301 agtggtcgtg atgtccggct gtattcctca ctggatgaaa cccgtactgc ggaaaaactt
     3361 gcccgccatc cctcctttac ggtggtttct gagcagataa aggcgcgtgc cggtgagaca
     3421 ttgctggaaa ccgctatcag tctgcagaaa gccgggcttc acacgccggc acagcaggcc
     3481 attcatctgg cccttcctgt gctggaaagt aaaaacctgg ccttcagcat ggtggacctg
     3541 ctgacagagg cgaagtcgtt tgctgcagaa ggaaccggtt ttgctgacct gggaggggaa
     3601 atcaatgcgc agataaaacg gggtgattta ctgtatgtgg atgtggcaaa aggctatggc
     3661 acaggcctgc tggtttcccg tgcgtcgtat gaggcagaaa agagcattct tcgccatatt
     3721 ctcgaaggta aggaggcggt cacgccgctg atggagagag tacctggcga actcatggag
     3781 aaactgacat caggacagcg cgccgccacc cgtatgatac tggaaacgtc tgaccgtttc
     3841 acggtggtgc agggctatgc cggtgtgggt aagaccacac agttccgggc ggtgatgtca
     3901 gccgtgaaca tgctgccgga gagtgaacgt ccccgtgtcg tggggctggg gcccacacac
     3961 cgtgcggtcg gggagatgcg cagcgccggc gtggatgcac agacactggc gtcctttctg
     4021 catgacacgc agcagcgcag cggagaaacg ccggatttca gcaacacgct gttccttctc
     4081 gatgagagct ctatggtggg caataccgac atggcacggg catacgccct gattgcggcc
     4141 ggtggcggtc gggctgtggc cagcggtgac acggaccagc tgcaggccat cgcgcccggt
     4201 cagcctttcc gtctccagca gacgcgcagt gctgccgatg tggtcatcat gaaggagatt
     4261 gtgcgtcaga cgccggaact gcgggaggcg gtatacagcc tgattaaccg ggatgtggaa
     4321 agggcgctgt ccgggcttga gcgtgtgaaa ccgtctcagg tgccacgtct ggagggcgca
     4381 tgggcaccgg agcactccgt gacggagttc agtcacagcc aggaagcgaa actggcagaa
     4441 gcgcagcaga aggcgatgct gaaaggcgag gcttttccgg atgtccccat gacactgtat
     4501 gaagccattg tccgcgacta taccggcagg acaccggaag cacgggagca gacgctgatt
     4561 gtcacgcacc tgaatgagga ccggcgcgta ctgaacagca tgattcatga tgcacgggaa
     4621 aaggccggag aactggggaa ggtgcaggtc atggtgcctg tcctgaacac agcgaatata
     4681 cgcgacgggg agctgcgtcg tctctccacc tgggagaata acccggatgc ccttgccctg
     4741 gtggataatg tgtatcaccg gattgccggt atcagcaagg atgacgggct gataaccctg
     4801 caggatgcgg aaggtaacac gcggctgatt tcgccccggg aggcggtggc tgaaggcgtc
     4861 acgctgtaca ccccggacac catccgggtg gggaccggtg acaggatacg cttcacgaag
     4921 agtgaccggg agcgcggtta tgtggccaac agcgtctgga cggtgacagc ggtttccggt
     4981 gacagtgtca cactttctga cggtcagcag acccgtgtga ttcgccccgg tcaggagcgg
     5041 gcagagcaac atattgacct ggcctatgcc atcaccgccc acggtgcaca gggggcgagt
     5101 gaaacctttg ccatcgcgct tgaaggcacg gaaggtaacc ggaaactgat ggccggcttt
     5161 gagtcagcct acgtggccct gtcgcgtatg aagcagcatg tgcaggcgta caccgataac
     5221 cgtcagggct ggacggatgc catcaacaat gccgtacaga aaggaactgc ccacgatgtg
     5281 tttgagccga aaccggaccg ggaggtcatg aatgcagagc ggctgttcag tacggcgcgg
     5341 gagctgcggg acgtggcggc aggccgtgcc gttctccgtc aggcggggct ggccggggga
     5401 gacagtccta cacggtttat tgctccggga cgtaaatatc cgcaaccgta tgtggcactg
     5461 ccggcgtttg accgtaacgg caagtccgcc ggtatctggc tgaacccgct gaccacggat
     5521 gacggaaacg ggctgcgggg attcagcggt gaaggtcggg taaaaggcag cggggatgcg
     5581 cagttcgtgg ccctgcaggg cagccgtaac ggagaaagcc tgctggctga taatatgcag
     5641 gatggcgtcc ggattgcccg tgataatcct gacagtggtg tggtggtaag aatcgccggt
     5701 gaaggccgtc cgtggaatcc cggtgccatc accggtggcc gcgtgtgggg ggatatcccg
     5761 gacaacagcg tccagccggg agccggaaat ggcgaaccgg tcacggcaga ggtactggca
     5821 cagcggcagg ctgaagaggc catccgccgt gaaacggaac gtcgcgcaga tgaaattgtc
     5881 cgtaaaatgg cagagaacaa acctgacctg ccggacggca aaacagagca ggctgtcagg
     5941 gaaattgccg gtcaggagcg tgaccgggct gccataactg aacgggaagc cgcgctgccg
     6001 gagagtgtac tgcgtgaacc acaacgggtg cgggaagcgg tccgggaagt tgcccgggaa
     6061 aatttgctgc aggagcgtct gcagcagatg gagcgggata tggttcgtga cctgcagaaa
     6121 gagaaaaccc cgggcggaga ctgatacagg aagacaaact ctgatgacaa cggataacac
     6181 gaacacgaca cgtaacgatt cactggctgc acggaccgat acctggttgc agtcatttct
     6241 ggtctggtca cccggacagc gggatatcat caaaacggtg gcactggtgc tgatggtact
     6301 ggaccacatc aatctgatat tccagctgaa gcaggaatgg atgtttctgg ccgggcgagg
     6361 agcctttccg ctttttgccc tggtgtgggg gctgaatctg tcccgtcatg cgcatatccg
     6421 gcaaccggcc attaaccggc tgtggggatg gggaattatt gcccagttcg catattatct
     6481 ggcaggcttt ccttggtatg aggggaatat cctgtttgcc tttgcagtgg cggcacaggt
     6541 gctgacgtgg tgtgaaacgc gcagtgggtg gcgtacagca gcggccatac tcctgatggc
     6601 cctgtggggg cctttgtccg gcaccagtta cggtattgcc gggttgctga tgctggcagt
     6661 cagcaaccgg ctgtaccggg cggaagacag agcggaacgt ctggcgctgg tcgcctgcct
     6721 gctggctgtt attccggcgc ttaaccttgc caccagtgat gcagcggcgg tggccggtct
     6781 tgtgatgacg gtgctgaccg ttggtctggt gttgtgtgcc ggaaaatcat taccccgttt
     6841 ctggcccgga gactttttcc cgacgttcta tgcctgtcat ctggctgtgc tgggcgttct
     6901 ggcgctgtga cgggtgtggt atctttggcc gcaagaggat gattcgtcag aggcagaaca
     6961 cagcatgata gagcagaagc gaccggtact gacactgaag cggaagacag agggaacagc
     7021 gcctgtccgc agccggaaaa ccatcatcaa tgtcaccacg ccaccaaagt ggaaggtgaa
     7081 aaagcagaag ctggccgaga aagcggcccg tgaagcagag ctggcggcaa aaaaagcgca
     7141 ggccagacag gcgctgtcca tttatctgaa cctgcccacg ctggatgagg ctgtgaatac
     7201 cctgaagccc tggtggccgg gattatttga cggtgacacg ccctggcttc tggcctgcgg
     7261 tatccgggac gtgttactgg aagacgtggc gcagcggaat atcccgctct cgcataaaaa
     7321 actgcgcagg gcgctgaagg ccatcacccg ttcagaaagc tatctgtgtg ccatgaaagc
     7381 cggtgcctgc cggtatgaca cggaagggta tgtgacggag catatttctc aggaggagga
     7441 agcgtatgcg ggagcgcgtc tggcgaaaat tcgtcgccag aaccggataa aggcagaact
     7501 tcaggccgtg cttgatgaga aataaaaaaa gcctccccca gagagaggag gcagggaaat
     7561 aaggcttaaa aggaaataac ttccgcttcc gaaaggaagc cagtccactg aattatttat
     7621 cacaataata ctgacaacta aagggggaca agaaaatgaa tggattcaga aacagttcac
     7681 ggaacggcca ggtctggcgt taccagcgtg ccggcgggcg ggccgttatt ctggaagtca
     7741 gtggacgctg gatggaagcg gcagaagcat ggcgacgggc tgcctgtgtt gcgcctagga
     7801 cagactggca gcagtttgcc cggaaaaggg ctgaacactg ccaccggcgc tgccggggaa
     7861 gggtgtaacg aaaaaagcag ccttcattac tctaggagtc agaaggctga aaggagttta
     7921 tgagatttgc ttcttaatat ccggtcgggc agtatgaccg gacagttctg agtagcacag
     7981 ggtcttttcc gcattaagtc gattttttat attttgtgat taatttcaca aaataaggtg
     8041 ttattcaatg tgtgctgcac tattcagggg ggctgaaata caggtggcat ttatctgata
     8101 tggagaataa catgaggaaa tatattccac tggtattatt tatcttttca tggccggtat
     8161 tgtctgcaga tattcatgga cgggttgttc gtgtactgga cggtgatacc atcgaggtca
     8221 tggactcact gaaggcggtg cggatcaggc tggtaaatat tgatgcaccg gaaaagaaac
     8281 aggattatgg ccggtggtca acagacatga tgaaatcact ggtcgcagga aaaacggtta
     8341 ccgtcacat
//
LOCUS       DADMGC010000154         8344 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.154, whole genome shotgun sequence.
ACCESSION   DADMGC010000154 DADMGC010000000
VERSION     DADMGC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.154"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(80..871)
                     /gene="aadA1"
                     /locus_tag="JNZ73_003593"
     CDS             complement(80..871)
                     /gene="aadA1"
                     /locus_tag="JNZ73_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY8733726.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(964..1437)
                     /locus_tag="JNZ73_003594"
     CDS             complement(964..1437)
                     /locus_tag="JNZ73_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HAY8733727.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVVIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1597..2610
                     /gene="intI1"
                     /locus_tag="JNZ73_003595"
     CDS             1597..2610
                     /gene="intI1"
                     /locus_tag="JNZ73_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY8733728.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2813..3163
                     /locus_tag="JNZ73_003596"
     CDS             2813..3163
                     /locus_tag="JNZ73_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HAY8733729.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            3289..3849
                     /locus_tag="JNZ73_003597"
     CDS             3289..3849
                     /locus_tag="JNZ73_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8733730.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3852..6818
                     /locus_tag="JNZ73_003598"
     CDS             3852..6818
                     /locus_tag="JNZ73_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HAY8733731.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLEGAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6885..7262
                     /locus_tag="JNZ73_003599"
     CDS             6885..7262
                     /locus_tag="JNZ73_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8733732.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7463..8122)
                     /locus_tag="JNZ73_003600"
     CDS             complement(7463..8122)
                     /locus_tag="JNZ73_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HAY8733733.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1841 a         2433 c         2271 g         1799 t
ORIGIN      
        1 caattatgtg cttagtgcat ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc
       61 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
      121 gaacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
      181 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
      241 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
      301 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
      361 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
      421 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
      481 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
      541 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
      601 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
      661 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
      721 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
      781 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
      841 tcgatacttc ggcgatcacc gcttccctca tgatgtttaa cgcctggcac agcggatcgc
      901 aaacctggcg cggcttttgg tacaaaaggc gtgacaggtt tgcgaatccg ttgctgccac
      961 ttgttaaccc ttttgccaga tttggtaact ataatttatg ttagaggcga agtcttgggt
     1021 aaaaactggc ctaaaattgc tggggatttc aggaaagtaa acatcacctt ccggctcgat
     1081 gtctattgta gatatatgta gtgtatctac ttgatcgatc aggcttttgt atatctcccc
     1141 accacctgaa acaatgacat gatccgttat tttctttagg ttggttaaag catctttaat
     1201 tgatggaaag atcactacgt tctcattgtc agatgtaaaa cttgaacgtg ttacgaccgc
     1261 atactttcgg ttgggtaatg ctcccattga ttcaaaagtc ttgcgtccaa ccaacagcca
     1321 ttggttatag gtaatagctt taaacaggag ctgttcacct ttggcactcc atggaatatc
     1381 agggccattc ccgataactc cattcttcga tatagctacc attagtgata gtttcacaat
     1441 tcttcctcag aggttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
     1501 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     1561 atagactgta ccccaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     1621 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat
     1681 tacagcttac gaaccgaaca ggcttatgtc cactgggttc gtgccttcat ccgtttccac
     1741 ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg
     1801 gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc
     1861 ttctacggca aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg
     1921 ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt
     1981 ctggaaggcg agcatcgttt gttcgcccag cttctgtatg gaacgggcat gcggatcagt
     2041 gagggtttgc aactgcgggt caaggatctg gatttcgatc acggcacgat catcgtgcgg
     2101 gagggcaagg gctccaagga tcgggccttg atgttacccg agagcttggc acccagcctg
     2161 cgcgagcagc tgtcgcgtgc acgggcatgg tggctgaagg accaggccga gggccgcagc
     2221 ggcgttgcgc ttcccgacgc ccttgagcgg aagtatccgc gcgccgggca ttcctggccg
     2281 tggttctggg tttttgcgca gcacacgcat tcgaccgatc cacggagcgg tgtcgtgcgt
     2341 cgccatcaca tgtatgacca gacctttcag cgcgccttca aacgtgccgt agaacaagca
     2401 ggcatcacga agcccgccac accgcacacc ctccgccact cgttcgcgac ggccttgctc
     2461 cgcagcggtt acgacattcg aaccgtgcag gatctgctcg gccattccga cgtctctacg
     2521 acgatgattt acacgcatgt gctgaaagtt ggcggtgccg gagtgcgctc accgcttgat
     2581 gcgctgccgc ccctcactag tgagaggtag ggcagcgcaa gtcaatcctg gcggattcac
     2641 tacccctgcg cgaaggccat cggtgccgca tcgaacggcc ggttgcggaa agtcctccct
     2701 gcgtccgctg atggccggca gcagcccgtc gttgcctgat ggatccaacc cctccgctgc
     2761 tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga caatggaggt
     2821 ggtagccgag ggtgtggaaa cacccgactg ccttgcgtgg ttgcggcagg cgggttgcga
     2881 cacggtgcag ggtttcctgt tcgccaggcc gatgccggcg gcggccttcg tcggcttcgt
     2941 caaccaatgg aggaacacca ccatgaacgc caatgaaccg agcaccagtt gctgcgtgtg
     3001 ctgcaaggaa atcccgctcg atgccgcctt cacgccggaa ggggccgagt acgtggagca
     3061 tttctgcggg ctggagtgct atcagcgctt ccaggcgcgg gccagcactg cgaccgaaac
     3121 cagcgtcaaa ccggacgctt gtgattcgcc gccgtcaggt tgaggcatac cctaacctga
     3181 tgtcagatgc catgtgtaaa ttgcgtcagg ataggattga attttgaatt tattgacata
     3241 tctcgttgaa ggtcatagag tcttccctga cattttgcag ggaattccat gactggacag
     3301 cgcattgggt atatcagggt cagcaccttc gaccagaacc cggaacggca actggaaggc
     3361 gtcaaggttg atcgcgcttt tagcgacaag gcatccggca aggatgtcaa gcgtccgcaa
     3421 ctggaagcgc tgataagctt cgcccgcacc ggcgacaccg tggtggtgca tagcatggat
     3481 cgcctggcgc gcaatctcga tgatttgcgc cggatcgtgc aaacgctgac acaacgcggc
     3541 gtgcatatcg aattcgtcaa ggaacacctc agttttactg gcgaagactc tccgatggcg
     3601 aacctgatgc tctcggtgat gggcgcgttc gccgagttcg agcgcgccct gatccgcgag
     3661 cgtcagcgcg agggtattgc gctcgccaag caacgcgggg cttaccgtgg caggaagaaa
     3721 tccctgtcgt ctgagcgtat tgccgaactg cgccaacgtg tcgaggctgg cgagcaaaag
     3781 accaagcttg ctcgtgaatt cggaatcagt cgcgaaaccc tgtatcaata cttgagaacg
     3841 gatcagtaaa tatgccacgt cgttccatcc tgtccgccgc cgagcgggaa agcctgctgg
     3901 cgttgccgga ctccaaggac gacctgatcc gacattacac attcaacgat accgacctct
     3961 cgatcatccg acagcggcgc gggccagcca atcggctggg cttcgcggtg cagctctgtt
     4021 acctgcgctt tcccggcgtc atcctgggcg tcgatgaact accgttcccg cccttgttga
     4081 agctggtcgc cgaccagctc aaggtcggcg tcgaaagctg gaacgagtac ggccagcggg
     4141 agcagacccg gcgcgagcac ctgagcgagc tgcaaaccgt gttcggtttc cggcccttca
     4201 ccatgagcca ttaccggcag gccgtccaga tgctgaccga gctggcgatg caaaccgaca
     4261 aaggcatcgt gctggccagc gccttgatcg ggcacctgcg gcggcagtcg gtcattctgc
     4321 ccgccctcaa cgccgtcgag cgggcgagtg ccgaggcgat cacccgtgct aaccggcgca
     4381 tctacgacgc cttggccgaa ccactggcgg acgcgcatcg ccgccgcctc gacgatctgc
     4441 tcaagcgccg ggacaacggc aagacgacct ggttggcttg gttgcgccag tctccggcca
     4501 agccaaattc gcggcatatg ctggaacaca tcgaacgcct caaggcatgg caggcactcg
     4561 atctgcctac cggcatcgag cggctggttc accagaaccg cctgctcaag attgcccgcg
     4621 agggcggcca gatgacaccc gccgacctgg ccaaattcga gccgcaacgg cgctacgcca
     4681 ctctcgtggc gctggccacc gagggcatgg ccaccgtcac cgacgaaatc atcgacctgc
     4741 acgaccgcat cctgggtaag ctgtttaacg ctgccaagaa taagcatcag cagcagttcc
     4801 aggcgtcagg caaggccatc aacgccaagg tacgtctgta cgggcgcatc ggtcaggcgc
     4861 tgatcgacgc caagcaatca ggccgcgatg cgtttgccgc catcgaggcc gtcatgtcct
     4921 gggattcctt tgccgagagc gtcaccgagg cgcagaagct cgcgcaaccc gatgacttcg
     4981 atttcctgca tcgcatcggc gagagctacg ccaccctgcg ccgctatgca ccggaattcc
     5041 ttgccgtgct caagctgcgg gccgcgcccg ccgccaaaaa cgtgcttgat gccattgagg
     5101 tgctgcgcgg catgaacacc gacaacgccc gcaagctgcc agccgatgca ccgaccggct
     5161 tcatcaagcc gcgctggcag aaactggtga tgaccgacgc cggcatcgac cggcgctact
     5221 acgaactgtg cgcgctgtcc gagttgaaga actccctgcg ctcgggcgac atctgggtgc
     5281 agggttcacg ccagttcaag gacttcgagg actacctggt accgcccgag aagttcacca
     5341 gcctcaagca gtccagcgaa ttgccgctgg ccgtggccac cgactgcgaa caatatctgc
     5401 atgagcggct gacgctgctg gaagcacaac ttgccaccgt caaccgcatg gcggcagcca
     5461 acgacctgcc ggatgccatc atcaccgagt cgggcttgaa gatcacgccg ctggatgcgg
     5521 cggtgcccga caccgcgcag gcgctgatag accagacagc catggtcctg ccgcacgtca
     5581 agatcaccga actgctgctc gaagtcgatg agtggacggg cttcacccgg cacttcacgc
     5641 acttgaaatc gggcgatctg gccaaggaca agaacctgtt gttgaccacg atcctggccg
     5701 acgcgatcaa cctgggcctg accaagatgg ccgagtcctg ccccggcacg acctacgcga
     5761 agctcgcttg gctgcaagcc tggcataccc gcgacgaaac gtactcgaca gcgttggctg
     5821 aactggtcaa cgctcagttt cggcatccct ttgccgggca ctggggcgat ggcaccacat
     5881 catcatcgga cggacagaat ttccgaaccg ctagcaaggc aaagagcacg gggcacatca
     5941 acccaaaata tggcagcagc ccaggacgga ctttctacac ccacatctcc gaccaatacg
     6001 cgccattcca caccaaggtg gtcaatgtcg gcctgcgcga ctcaacctac gtgctcgacg
     6061 gcctgctgta ccacgaatcc gacctgcgga tcgaggagca ctacaccgac acggcgggct
     6121 tcaccgatca cgtcttcgcc ctgatgcacc tcttgggctt ccgcttcgcg ccgcgcatcc
     6181 gcgacctggg cgacaccaag ctctacatcc cgaagggcga tgccgcctat gacgcgctca
     6241 agccgatgat cggcggcacg ctcaacatca agcacgtccg cgcccattgg gacgaaatcc
     6301 tgcggctggc cacctcgatc aagcagggca cggtgacggc ctcgctgatg ctcaggaaac
     6361 tcggcagcta cccgcgccag aacggcttgg ccgtcgcgct gcgcgagttg ggccgcatcg
     6421 agcgcacgct gttcatcctc gactggctgc aaagcgtcga gctacgccgc cgcgtgcatg
     6481 ccgggctgaa caagggcgag gcgcgcaatg cgctggcccg tgccgtgttc ttcaaccgcc
     6541 ttggtgaaat ccgtgaccgc agtttcgagc agcagcgcta ccgggccagc ggcctcaacc
     6601 tggtgacggc ggccatcgtg ctgtggaaca cggtctacct ggagggtgcg gcgcatgcgt
     6661 tgcgcggcaa tggtcatgcc gtcgatgact cgctattgca gtacctgtcg ccactcggct
     6721 gggagcacat caacctgacc ggtgattacc tatggcgcag cagcgccaag atcggcgcgg
     6781 ggaagttcag gccgctacgg cctctgcaac cggcttagcg tgctttattt tccgttttct
     6841 gagacgaccc ctattatggc gaaggtatca ttcaggaagc gttgatgaag gattatctct
     6901 gtaaaaagtt attcaaccgg ctttccggta ccctggtgat cagagcgcgc tgcggcaata
     6961 acattactgg cctggcatgt tgcaatattc tttatccctc gccccgatac agcggtcagc
     7021 tgcatattaa agagctgtat gtttctcagt gcgatcgaaa taagggcacg ggtaaagcga
     7081 taatgcgctt tatagcccgg cttgcgcttg aacaggaatg ccttagcctt agctggaacg
     7141 ctgaaaaatc caaccccggc gctaaccgtt tttatcaggc tctgggaggc agaataaatg
     7201 atcatatcgt caattattac ctccacgggg agagcctgag caaactggcc tcaggcattt
     7261 gagaagcaca cggtcacact gcttccggta gtcaataaac cggtaaacca gcaatagaca
     7321 taagcggcta tttaacgacc ctgccctgaa ccgacgaccg ggtcgaattt gctttcgaat
     7381 ttctgccatt catccgctta ttatcactta ttcaggcgta gcaaccaggc gtttaagggc
     7441 accaataact gccttaaaaa aattacgccc cgccctgcca ctcatcgcag tactgttgta
     7501 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     7561 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     7621 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     7681 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     7741 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     7801 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     7861 cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat
     7921 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     7981 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     8041 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     8101 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     8161 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     8221 gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac
     8281 caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg
     8341 cgtc
//
LOCUS       DADMGC010000155         8318 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.155, whole genome shotgun sequence.
ACCESSION   DADMGC010000155 DADMGC010000000
VERSION     DADMGC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.155"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            332..3181
                     /locus_tag="JNZ73_003601"
     CDS             332..3181
                     /locus_tag="JNZ73_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY8733734.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYATSRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRMGDLRNQ
                     SKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLYIDSTHA
                     SPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNCVNANGT
                     ANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLNHYESLL
                     GRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGWNNGVGV
                     SAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3281..5209)
                     /gene="dhaR"
                     /locus_tag="JNZ73_003602"
     CDS             complement(3281..5209)
                     /gene="dhaR"
                     /locus_tag="JNZ73_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HAY8733735.1"
                     /translation="MRDMSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGV
                     TFASIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSA
                     LGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRL
                     TGTIALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDG
                     VISWDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEA
                     TFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDD
                     PQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGD
                     AALAEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITR
                     LDARRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPAL
                     VNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSD
                     LPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTL
                     WRKMKQHGIDAGQFKRRV"
     gene            5428..6498
                     /gene="dhaK"
                     /locus_tag="JNZ73_003603"
     CDS             5428..6498
                     /gene="dhaK"
                     /locus_tag="JNZ73_003603"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAY8733736.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDVPVHTPALNWGK"
     gene            6509..7141
                     /gene="dhaL"
                     /locus_tag="JNZ73_003604"
     CDS             6509..7141
                     /gene="dhaL"
                     /locus_tag="JNZ73_003604"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HAY8733737.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7152..>8318
                     /gene="dhaM"
                     /locus_tag="JNZ73_003605"
     CDS             7152..>8318
                     /gene="dhaM"
                     /locus_tag="JNZ73_003605"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAY8733738.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEE"
BASE COUNT         2173 a         2066 c         2076 g         2003 t
ORIGIN      
        1 cataactaat taggattatt acaaaaaatc ttcaccaaat tttttaaccg ctgtttgttg
       61 taatattttt gccatattca tagtaacgaa ttggaataag taacgacaac ttagggaata
      121 actcgatata aactcacctt tgtaaggggg gtgttaaata tatattaatt cctgtgttcc
      181 tgattttgcc cggtaataga gaattattct cagagaagct catcatgatt gcaccataca
      241 gatatcatga tgataagagg atattattca ttgacttaaa caaaatgaca aaatccatag
      301 acccatagcc tgggtactat ataggagtgg tatgggcatc aaacaacaca atgggaatac
      361 caaagccgat cgtctcgctg aattaaaaat ccgttcgccc tcaattcaac tgataaaatt
      421 tggcgctatt ggtttgaatg caattatctt ttcccccctg ctgatagctg ctgatacagg
      481 aagtcaatat ggcaccaata ttactattaa tgatggtgac agaattacag gagataccgc
      541 cgatccatca ggaaacctct atggtgtaat gaccccagca ggaaacacgc ctggcaatat
      601 caacctgggt aatgatgtca ccgtcaatgt caacgacgcc tctggatatg caaaaggaat
      661 cattattcag ggcaaaaaca gctccctgac agctaaccga ctcacagtag atgttgttgg
      721 tcaaacctct gccatcggca ttaacttaat tggtgactat acccatgctg acttaggcac
      781 aggcagcacc attaagagta acgatgacgg catcattatt gggcatagct caacactaac
      841 agccactcaa ttcaccattg aaaactcgaa cggtataggc ctaaccatca atgactatgg
      901 caccagtgtc gatcttggaa gcggaagtaa aatcaagacc gatggaagta caggtgttta
      961 tatcggtggt ctcaacggca ataacgccaa tggtgctgcg cgttttacgg cgacagacct
     1021 gacaatcgat gttcagggct acagcgccat ggggataaac gtacagaaaa actctgttgt
     1081 cgatctcgga acaaacagta ccattaaaac caatggcgat aatgctcacg gcctctggag
     1141 ctttggccag gttagcgcga atgcactcac tgttgatgta actggagccg cggccaatgg
     1201 cgtcgaagtt cgtggtggta caaccactat cggtgcagat agccatattt cttccgcgca
     1261 gggcggtggc ctcgtcacca gtggttcaga cgcgacaatc aatttttctg gcacggcagc
     1321 gcaacgaaac agcatctttt ccggcggttc ttatggtgcc tcggcccaga cggcaacggc
     1381 tgttatcaac atgcaaaata ccgatattac ggttgatcgt aatggcagtc tggcgctggg
     1441 tttgtgggcg ctcagcggcg gtagaataac cggagacagt ttggctatca ccggcgcggc
     1501 aggagccaga gggatttatg ccatgaccaa cagccagatc gacctcacga gcgatctggt
     1561 cattgatatg agtacacccg accagatggc catcgcaacg caacatgacg atggttatgc
     1621 caccagccgc atcaacgcct cgggtcgtat gcttatcaac ggtagcgttc tttccaaagg
     1681 tgggctaatc aatctggata tgcaccctgg gtcggtttgg acaggttcct ccctcagcga
     1741 taatgtcaat ggcgggaaac tggacgttgc aatgaataac agcgtctgga acgtaacaag
     1801 taattctaat ctcgacacgc tggcgctgag ccattcaact gtcgattttg ccagccacgg
     1861 gtcaactgcc ggcacattta ccacattaaa cgtagagaac ctgagcggta acagtacctt
     1921 tattatgcgt gctgatgttg ttggcgaggg taatggcgtt aataatagag gggatttatt
     1981 gaatatcagc gggagtagtg ctggtaatca cgtattggct atccgcaacc agggcagcga
     2041 ggccacaacg ggaaatgaag ttctgacagt ggtaaaaacc actgacggcg cggcctcgtt
     2101 cagcgcgtct tctcaggttg agttgggggg atatctgtac gatgtgcgta aaaatggcac
     2161 taactgggag ctttacgctt ccgggacagt tccggaaccg actcctaatc ctgaacccac
     2221 accagctccc gctcagcctc ccatagtcaa ccccgatcct acgcctaaac ccaccacgac
     2281 cgcagatgct ggcggcaatt atctcaatgt cggttactta ttgaactatg ttgaaaaccg
     2341 tacgctgatg caacggatgg gtgacttgcg aaatcagagt aaagacggta atatctggtt
     2401 gcgcagttat gggggaagcc tggactcctt tgccagtggc aaactgagcg gctttgacat
     2461 gggttacagc ggtatccagt ttggtgggga taaacgtctc tctgatgtaa tgccgttgta
     2521 tgtcggtctg tatattgact caacacatgc atcgccggac tatagcggag gcgacggtac
     2581 cgcacgttca gactacatgg gaatgtacgc cagttacatg gcacaaaacg gtttttacag
     2641 cgatctcgtt ataaaagcat cgcgccagaa aaatagtttc cacgtactgg acagtcagaa
     2701 caactgcgtt aacgccaacg gcactgcgaa tggaatgagc atctccctgg aagccgggca
     2761 gaggttcaac ctgtccccta ctggttatgg gttctatata gagccgcaaa cccagcttac
     2821 atacagccac cagaatgaga tggctatgaa ggcgagtaat ggcctcaata tacatctgaa
     2881 tcactacgaa tcgctgctgg ggcgtgccag catgatactg gggtatgaca tcaccgcagg
     2941 caacagccag ctgaatgtct atgtgaagac tggcgctatc cgcgagtttt caggggatac
     3001 cgaatatctg ttgaacgact cccgggagaa gtacagtttc aaaggtaatg gctggaataa
     3061 cggcgtggga gtcagtgcac agtataacaa acagcacaca ttctatctcg aagcggatta
     3121 cacgcagggt aacctctttg atcagaagca agtcaacgga ggatatcgct tcagctttta
     3181 agtgcgggat agacaagccc ttaaacgatg ctgaaaattg ttagcgtgcg ccggaatgaa
     3241 atacagatca ctctgatgta tcagaaatcg tttctgtctt tcatacccgg cgcttaaact
     3301 gccctgcatc aatgccatgt tgcttcattt tccgccacag cgtagtgcgg ccgatcccaa
     3361 gtaaagccga catttcctga atgcgaccgc ctgtgacctg ggctgcgtta ataattgcct
     3421 ctttttcaac ttccgcaaat gacagactgg tggaaaggcg ggtggcgctg acatcatctg
     3481 tcgcctgctc ggtaaacaga tgttcaggta aatcactgac gcgaatgcgc ccgttatcac
     3541 tactcagagc aagattctcg atgacgctgt aaagttcaaa atcgttgcct ggccatgcac
     3601 aagaaactag gcgagcgagg gcatcgtcat caattttcag ccgcgtagag aagcgttttt
     3661 caagactgcg taatttgtta ttcaccagcg ccggaatgct gccacgccgc atacgcagag
     3721 gcgggatggt aatttcaaat gcatgcagcg cgtaatacag ctggcgacta aaacgatttt
     3781 gttccaccag cattgcgagg tccgcggtcg ttgttgcaat cactttgaca tcaattggta
     3841 ttaaacgccg cgcatccagt cgcgtgataa ccccctgctt gataacctgc agcaaagcag
     3901 actgtaactc caccgccaga tattcaatct tttcaagaaa cagcgtgccg ccgtgtgcca
     3961 gttccagccg actcagacgg ccattttcat tgtccgtgcg atcgccacca ataaattctt
     4021 ccgccagcgc agcatcacca tataactcac aattgacggc gatataagga cctgcagcac
     4081 gctcgctttc attatgaatt gcctgactta gcagtgcctt gcccacgccc tcttctccac
     4141 aaagcaggac aggaaagcta ctgcgcgccg cctggcgacc aaaatgaatc aagcggcggg
     4201 tttgcggatc gtcctgtggc atatgagcga aggtatggct gacttttcct aattgactgg
     4261 tcattaactg ccgcatctgt tccacaggat ggagcaacaa aataaagctg gttccctgcg
     4321 tttctattat cggtttaagg gttatcaccg catcaataaa ctggtgctgg ctttcaaagg
     4381 ttgcttctac gtgtttgagc ggatgtgcct gttttattgc ttgttgcaat acggcgggta
     4441 acgttaagag ttcagtgatt gcccgtccct gacttgccgt cgcgtcaagg cgcaagaccc
     4501 gcgccgcctg ggcattaata aattgcaaat taccctgctc gtcccagcta atcacgccat
     4561 catccatact ttctaacagg gcattaagtt gatttaaatg acggttagtt tcagcgagca
     4621 aactgtccgt cagcagtaaa ttaccgacct cgcgggcgat tgccaacgtc aacggcaaat
     4681 cagctgcggt agtttgctca accggacacg ccagcgctat tgttcccgtc aatcggccct
     4741 tgctgtcaaa caacggcgtt gcacaaaagg cccagttcca gagtgcctgt ttgaaatgtt
     4801 gatcggccat cgttttcacg gcctgaccag agatagccgc taacgatagc gcacaagtac
     4861 caataattcc ctcggcgcaa tacgtgccgt cattgaatcc cagtgcactt agctgctgca
     4921 aggtttgcgg atcgccatta cggctgagaa tgcaggcggt ttcatcgagg ataaacagcg
     4981 cacactctcg cggtgccata tattcccagg catcttccag cgcagcctgc ccgagcgtca
     5041 gcatcgcttt cttacgccga taaatagaag caaatgtcac gccctgggcc tgatgtggta
     5101 cgttccatgt ctcccgtttc atcagcttat tgcatcgctc ccaggaggtt gcaattaagg
     5161 gagatatgcc ccgaccatcg ttgttaaaag cgccactcat atcccgcatc ccttatgaca
     5221 aaaggtgttc cggctcgcaa ttttaagtat taatacacgt aaaagtttca atatggaaca
     5281 tttattaaag catatgtttc actatgaaac ataatttggg ctaattttct tttcccttgc
     5341 ctgatgcaca atggattcat cggcagtcag tggtcgccgt gtcgttgaac atcatccatg
     5401 ccctaccgta attgctggcg caaaataatg aaaaaattga tcaatgatgt gcaagacgta
     5461 ctggacgaac aactggcagg actggcgaaa gcgcatccat cgctgacact gcatcaggat
     5521 ccggtgtatg tcacccgagc tgatgcccct gttgcaggaa aagtcgccct gctgtcgggt
     5581 ggcggcagcg gacacgagcc gatgcactgt ggttatatcg gtcaggggat gctttcgggg
     5641 gcctgtccgg gcgaaatttt cacctcaccg acgcccgata aaatctttga atgctccatg
     5701 caaattgatg gcggcgaagg tgtactgttg attatcaaaa attacaccgg cgatattctt
     5761 aactttgaaa cagcgaccga gttactgcac gatagcggcg taaaagtgac cactgtggtc
     5821 attgatgacg acgttgcggt aaaagacagt ctttataccg ccgggcgacg cggcgttgcc
     5881 aacaccgtat taattgaaaa actcgtaggc gcagcggcgg agcgtggcga ctcactggac
     5941 gcctgtgcgg aactggggcg taagttgaat aatcaaggcc actcaatagg tatcgctctc
     6001 ggtgcctgta ccgttcctgc cgcgggcaaa ccttctttta cccttgcgga taatgagatg
     6061 gagtttggcg tcggcattca tggtgagcca ggtattgacc gccgcccttt ctcttccctt
     6121 gatcaaaccg tcgatgaaat gttcgacacc ctgctggaac atggctcata ccatcgcact
     6181 ttgcgtttct gggattatca acaaggcagc tggcaggaag aaccacaaac caaacaaccg
     6241 ctccagtctg gcgatcgggt gattgcactg gttaacaatc ttggcgcaac tccgctttct
     6301 gagctgtacg gcgtctataa ccgcctgacc acacgttgcc agcaagcggg attgactatc
     6361 gaacgtaatt taattggcgc gtactgcacc tcactggata tgaccggttt ctcaatcacc
     6421 ttactgaaag ttgatgacga aacgctggct ctctgggacg tcccggtcca caccccggcc
     6481 cttaactggg gtaaataagg agaaagcaat gtcactgagc agaactcaaa ttgttaactg
     6541 gctcactcgt tgtggcgata ttttcaacac cgagagcgag tatcttaccg ggctggatcg
     6601 cgagattggc gacgctgacc acgggctaaa tatgaaccga ggctttagca aagtggtgga
     6661 aaaactccct gctatcgcag ataaagatat cggtttcatt ctcaaaaata ccggtatgac
     6721 gctgctttcc agcgtcggtg gtgccagtgg tccgctgttc ggtaccttct ttatccgcgc
     6781 cgcacaggcg acccaggcac ggcaaagcct gacactggaa gagctttatc agatgttccg
     6841 cgatggcgcg gacggcgtaa tcagtcgcgg gaaagccgaa cctggcgata aaaccatgtg
     6901 tgatgtgtgg gtgccggtgg tggaatcgtt acgtcagtcc agcgagcaaa atctctctgt
     6961 tccggtggcg ctcgaagctg ccagtagcat cgccgaatcc gcggcacaaa gtacgattac
     7021 gatgcaagcc cgcaaaggcc gcgccagtta tctcggtgaa cgcagtattg gtcaccagga
     7081 tcccggcgcg acctcggtga tgtttatgat gcaaatgttg gcgttagccg caaaagagta
     7141 aggaattggt gatggtaaac ctggtcatag tttcacatag cagccgactg ggagaaggtg
     7201 tcggtgaatt agcccgtcag atgttaatga gtgatagttg taaaatcgcc attgccgcgg
     7261 gaattgacga tccacaaaat cccattggta ccgatgccgt caaagtgatg gaggccatcg
     7321 aatctgttgc tgatgccgac catgtgctgg tcatgatgga tatgggtagc gcattattga
     7381 gtgctgaaac tgcgctggaa ttgctggctc ccgagatcgc cgcaaaagta cgtttgtgtg
     7441 ctgcgccgtt ggtcgaaggt acactggcag caacggtcag cgcggcctcg ggggcggata
     7501 tcgacaaagt tatctttgac gctatgcatg cgctggaagc caaacgtgaa caactgggtt
     7561 taccgtcctc cgacactgaa atctctgaca catgtcctcc gtacgatgaa gaagcccgtt
     7621 ctctggcggt ggtcataaaa aaccgtaacg gcctgcatgt acgtccggcc tcccggctgg
     7681 tttatacctt atcgacattt aatgccgata tgttgctgga aaaaaacggc aaatgcgtca
     7741 caccagagag tattaaccag attgcgttac tacaagttcg ctataacgat acgctgcgcc
     7801 tgattgcgaa agggccagaa gctgaagagg cactgatcgc tttccgtcag ctggctgaag
     7861 ataactttgg tgaaacggag gaagtcgctc cacctactct gcgtcccgtt ctgcctgttt
     7921 cgggtaaagc cttttattat caaccagttt tatgtacggt acaggcaaaa tcaaccctga
     7981 ccgcggaaga agaacaagat cgattacgcc aggctattga cttcacgtta ttagatctga
     8041 tgacgttaac agcgaaagca gaagccagcg ggcttgacga tattgccgca atcttttctg
     8101 gtcaccatac actgttaggt gatccggaac tgctggcggc ggcaagcgaa ctccttcagc
     8161 atgaacattg cacggcagaa tatgcctggc agcaagttct taaagaactt agccagcaat
     8221 accagcaact ggatgatgaa tatctacaag ctcgctatat tgatgtggac gatcttctgc
     8281 atcgcaccct ggtccacctg acccaaacga aagaagaa
//
LOCUS       DADMGC010000156         8228 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.156, whole genome shotgun sequence.
ACCESSION   DADMGC010000156 DADMGC010000000
VERSION     DADMGC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.156"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            59..460
                     /locus_tag="JNZ73_003606"
     CDS             59..460
                     /locus_tag="JNZ73_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY8733739.1"
                     /translation="MSSNFCERLEQWGNKSVNNGEERAIAVERIKEAYNSNMASLDLS
                     YLDLSELPPIPSTVNTLNLENNCLTCLDLADIASLVNINLSVNKINTITFPKEPKLEN
                     IYIDHNNLESLDLKNQHSLVNLEAQNNSLKK"
     gene            570..1901
                     /locus_tag="JNZ73_003607"
     CDS             570..1901
                     /locus_tag="JNZ73_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY8733740.1"
                     /translation="MITDLILHNHPKMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYTIKKAYIPYNIHTYKTE
                     EVVNQRNIKVKNMTLDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            2225..3613
                     /locus_tag="JNZ73_003608"
                     /pseudo
     CDS             2225..3613
                     /locus_tag="JNZ73_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
     gene            3695..7328
                     /locus_tag="JNZ73_003609"
                     /pseudo
     CDS             3695..7328
                     /locus_tag="JNZ73_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
     gene            7325..>8228
                     /gene="narH"
                     /locus_tag="JNZ73_003610"
     CDS             7325..>8228
                     /gene="narH"
                     /locus_tag="JNZ73_003610"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY8733741.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEAASTEREVDLYRVGSLTAA"
BASE COUNT         2196 a         2036 c         1922 g         2074 t
ORIGIN      
        1 ttgtgtaaaa aataatgcca gttatcaact taataacgca ttacctaaca aagaaaccat
       61 ctccagcaat ttttgtgaac gactagaaca atggggtaat aaatcggtta ataatggtga
      121 agaaagagca attgccgtgg agcgaattaa agaagcttac aattcgaaca tggcatctct
      181 tgatttgtca tatcttgatt tgagtgaatt accgcctatc ccctcgacag ttaatacgtt
      241 aaatctggaa aacaattgtc taacttgtct tgacttagct gacattgcca gtctcgttaa
      301 tatcaacctc agcgttaata aaattaacac gataaccttt ccaaaagaac caaaactgga
      361 aaacatttat attgatcata acaatctgga aagtttagat ttaaaaaatc agcattcatt
      421 ggttaatctg gaagcgcaaa ataacagcct aaaaaaatga tatttctgat agttataaac
      481 tgaaatttct taatcttgat tataacaagc tagcatcgct ggatgtctcc cggcaagaat
      541 ctctgattga gttaagtgcc catcacaata tgatcactga ccttatatta cacaatcatc
      601 ccaagatgaa aacaatcact ttaaacgaca accatattgc acatttaaac gccaaaaaca
      661 ctacaaaact ggaatattta aacttaagca ataacaattt actgccaacc aatgacattg
      721 atcaactaat atcatcaaaa catctttggc atgtattagt taacggcatc aacaatgatc
      781 cacttgcaca aatgcagtac tggactgcag taagaaatat aattgatgac actaatgaag
      841 tgaccattga tttatcagga cttaatttaa ccactcaacc accagggctg caaaacttca
      901 cctctatcaa tcttgataat aaccaactca cacattttga tgcaaccaac tacgatagac
      961 tcgtaaagct aagtctgaat agtaatgctc ttgagtcaat aaattttcct caaggcagaa
     1021 atgtaagtat tacacatata tctatgaata ataatgctct cagaaatatt gatatagata
     1081 ggctttcatc agttacttat tttagtgcgg cacataatca actagagttt gtgcaattag
     1141 aatcttgcga atggctgcaa tacctgaatc tcagccataa tcaattaact gatattgttg
     1201 caggtaataa aaatgaactc ttactgctgg atctatccca taataaacta acaagtttac
     1261 acaatgtctt atttcccaac ttgaatacgt tacttattaa caacaatttg ctttctgaaa
     1321 ttaaaatatt ctatagcaac ttccgcaatg ttcagacatt aaacgctgct aacaaccagt
     1381 tgaaatatat aaatcttgat ttcctgactt atcttccatc tatcaaaagt ttaagactgg
     1441 acaataataa aataacccac attgatacta ataatacatc cgatattgga actttattcc
     1501 ccataataaa acagagcaaa aacttaaatt ttttaaatgt ttctgggaag aacaattgcc
     1561 ctactatgca gctcatgtta tttaatttgt tttccccagc acttaagctt aatactggcc
     1621 tggcaattct ttcgcctggt gcatttgaag ttcactctga cggaatagat gtggataacg
     1681 aattgtttca ctatactatt aaaaaagcat atatcccata taatatacac acttacaaga
     1741 cagaggaagt tgtaaaccag aggaatataa aagttaaaaa catgacctta gatgaaataa
     1801 acaatactta ctataataac gattattaca atcaggcaat aagagaggaa ccgatagact
     1861 ttctggacag atcgttttcc tccaattcat ggccttttta gtgaataaat catcactaat
     1921 tctataagtg tctggtcttg ttgagaatat agttaatatg taacccttta gattcacgat
     1981 ctgaagggtt gaaacatgac atacgttcaa agattaaccc ccgtttgaag gttagcttgt
     2041 tagttacatt tagtaacaca tatctggcta cttaatcccc attctgcccc cgcggttacg
     2101 ctttcttatc tattctttca atcagcactt cacccaattc atccttaatt ctaataactg
     2161 gctattgcgc cttacctcct gttttgtctt ttttcattac caatgtgtgc atgtgaggaa
     2221 caatatggca ctgcaaaatg agaaaaatag tcgttatctt ttacgcgact ggaaaccaga
     2281 aaatccggcc ttctgggaaa ataaaggaaa acatattgct cgaagaaacc tctggatatc
     2341 agtcagttgt ctacttcttg ccttctgtgt ctagatgcta tttagcgcag ttaccgttaa
     2401 tctcaataaa atcggtttta attttactac cgatcaactc tttttattaa ccgcattacc
     2461 ctccgtttct ggcgcattat tgcgtgttcc ctactccttt atggtgccta tattcggtgg
     2521 acgccaatgg ccggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat
     2581 tgccgtgcaa aatccgaata ctccttttgg gatatttatc gttatcgctt tgctatgcgg
     2641 ttttgcaggt gcaaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc
     2701 caaacaaggg agcactcttg ggattaatgg cggattagga aacttaggtg taagtgtaat
     2761 gcagctggtt gcaccgctgg tcatttttgt acctgtattt gcctttctcg gcgtcaatgg
     2821 cgtaccgcag gccgacggtt cggtgatgtc gctggcgaat gccgcatgga tttgggtacc
     2881 gctactggcg attgccacga tcgccgcctg gtcagggatg aatgatatcg ccagttcacg
     2941 cgcctcaatt gccgaccagc tccctgtctt acaacgcctg catctctggc tgctgagcct
     3001 gctttacctt gccaccttcg gttcgtttat cggtttttct gcgggttttg ccatgctggc
     3061 aaaaacccag ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttattgg
     3121 tgccatcgcg cggtcggttg gtggtgctat ttccgataaa ttcggcggcg tgcgggtgac
     3181 gttgatcaac tttattttta tggcgatttt cagtgccctg ctgttcctta ccttaccggg
     3241 cacaggctcc ggtaatttca tcgcctttta cgccgtattt atggggctgt ttctgaccgc
     3301 gggtctggga agtggttcta ctttccagat gatcgccgtc atctttcgcc agataaccat
     3361 ttatcgggtg aagatgaaag gcggtagtga tgagcaagct cagagagaag ccgtcaccga
     3421 aacggcggca gcgctgggct ttatctcagc cattggcgca gtgggcggct tttttattcc
     3481 gcaggcattt ggcatgtcgc tcaatatgac cggctctccg gtcggcgcga tgaaagtgtt
     3541 tttaatcttc tacatcgttt gtgtgctgct gacctggctg gtttatggtc ggcggaagtt
     3601 cagccaaaaa taagagatcg atgcctgatg tacaaccgtg tcgcatccga caaggcatca
     3661 gatcccaatt cattcctgga gcaggagtca tgtcatgagt aaacttttgg atcgctttcg
     3721 ctacttcaaa caaaagggcg aaacctttgc cgatggtcac ggacaggtga tgcatagcaa
     3781 ccgcgactgg gaggacagct atcgccagcg ttggcagttc gacaaaatcg tgcgttccac
     3841 ccacggcgtt aactgtacag gctcctgtag ctggaaaatc tacgttaaaa atggtctggt
     3901 gacctgggaa atccaacaga ccgactaccc gcgcactcgc cctgacctgc ccaatcatga
     3961 acctcgcggc tgcccgcgtg gcgcaagtta ctcctggtat ctttacagcg ctaaccgcct
     4021 gaaatacccg ctcattcgta aacgactgat tgaactgtgg cgcgaagccc tcaaacaaca
     4081 cagcgatccg gtactggcgt gggcatcgat tatgaacgat ccgcaaaagt gcctgagcta
     4141 caaacaagtg cgtgggcgcg gcgggtttat ccgctccaac tggcaggaac taaaccagct
     4201 gattgccgcc gctaacgtct ggaccatcaa aacctacggc ccggatcgcg ttgccggttt
     4261 ctcgccgatc ccggcgatgt cgatggtttc ttacgccgcc ggaacgcgtt atctgtcgct
     4321 gcttggcggc acctgtttaa gtttctacga ctggtattgc gacctgccgc ccgcctcgcc
     4381 gatgacctgg ggcgagcaaa ccgacgtacc ggaatctgcc gactggtata actccagcta
     4441 catcatcgct tgggggtcta acgtaccgca gacacgtacg ccggacgccc acttctttac
     4501 cgaagtacgc tacaaaggca ctaaaaccat cgccattacc cctgactact ctgaagtggc
     4561 caaattgtgc gaccagtggc tggcaccgaa acaaggcact gatagcgccc tggcgatggc
     4621 aatggggcat gtgattttaa aagagtttca tctcgataat cccagcgact actttatcaa
     4681 ctactgccgc cgctacagcg acatgccgat gctggtaatg ctggagcctc gcgacgatgg
     4741 tagctacgtt cccgggcgca tgatccgcgc atctgacctg gtggatggac tgggcgaaag
     4801 caacaatccg cagtggaaaa ccgtagcagt taataccgca ggtgaattgg tagtgccgaa
     4861 cggttcgatt ggtttccgct ggggagaaaa aggcaaatgg aatctggaat ccattgccgc
     4921 cggtacggaa accgaattgt cgttaaccct gctcggtcaa catgatgctg ttgcaggcgt
     4981 ggccttcccc tactttggcg gcattgaaaa tccgcatttt cgcagcgtaa aacacaatcc
     5041 ggtgctggtg cgccaattgc ccgttaaaaa cctgacgtta gtcgatggca acacctgtcc
     5101 agtggtcagc gtttatgatt tggtactggc gaattacggc ctcgatcgcg ggctggaaga
     5161 tgaaaacagc gcgaaagatt acgctgaaat caaaccgtac accccagcct ggggtgagca
     5221 aattaccggc gtaccgcgcc agtatattga aaccatcgcc cgcgaatttg ccgataccgc
     5281 ccataaaacg catgggcgct caatgattat cctcggcgca ggtgttaacc actggtatca
     5341 catggacatg aactaccgcg ggatgatcaa tatgctgatc ttctgcggtt gtgtcgggca
     5401 aagcggtggc ggctgggcac actatgtcgg ccaggaaaaa ctgcgcccac aaaccggctg
     5461 gttgccactg gcctttgcgc tcgactggaa ccgaccaccg cgccagatga acagcacctc
     5521 gtttttctac aatcattcca gccaatggcg ctatgaaaaa gtctctgctc aggagttact
     5581 ttcaccgctc gccgatgcca gtaagtacag cggtcatctg attgatttca acgttcgcgc
     5641 cgaacgtatg ggctggctac cttctgcgcc gcagctgggg cgtaacccgc tcgggattaa
     5701 agctgaagcc gacaaggccg gattatcccc cacagaattt accgcccagg cgctgaaatc
     5761 gggcgattta cgtatggcct gcgaacaacc agatagcggc agcaatcatc cgcgtaattt
     5821 gtttgtctgg cgctctaacc tgcttggctc ctccggcaaa ggccacgagt atatgcagaa
     5881 gtatctgctg gggaccgaaa gcgggattca gggcgaggaa ctcggtgcca gcgacgggat
     5941 caaaccggaa gaagtcgagt ggcaaactgc ggcgattgaa ggcaagctcg acctgctggt
     6001 gacgctcgac ttccgcatgt ccagtacctg cctgttctcc gatatcgtcc tgcccaccgc
     6061 cacctggtat gaaaaagacg atatgaacac ctcggatatg catccattta ttcatccgct
     6121 ttctgcggcg gtcgatccgg cctgggaatc acgcagcgac tgggaaatct acaaaggtat
     6181 tgccaaagca ttttcgcaag tgtgcgtggg ccatcttggc aaagaaaccg acgtggtatt
     6241 acaaccactg ctgcatgact ctccggcaga gctctcacag ccgtgtgaag tgctcgactg
     6301 gcgcaaaggc gaatgcgatc tgatcccggg taaaaccgcg ccgaatattg tggcggtgga
     6361 gcgcgactac cctgctacgt atgaacgctt tacctcgctc gggccattga tggacaaact
     6421 tggcaatggc ggtaaaggga tttcgtggaa tacccaggat gaaatcgatt tcctcggcaa
     6481 actcaattac accaagcgtg atggcccggc acaggggcgt ccgctgattg acaccgccat
     6541 tgacgcttca gaagtgattc tggcactggc accggaaacc aacggtcatg ttgcagtcaa
     6601 agcgtggcag gcgctgggcg agatcaccgg gcgcgaacat acccatctgg cgctgcacaa
     6661 agaggacgag aagattcgct ttcgcgatat tcaggcgcag ccgcgtaaaa ttatctccag
     6721 ccccacatgg tccggtctgg aaagcgatca cgtatcctat aacgcgggat acaccaacgt
     6781 tcatgagtta attccgtggc gcacgctgtc gggacgccag cagctctatc aggatcatcc
     6841 gtggatgcgt gcttttggtg aaagcctggt ggcttatcgc ccgcctatcg acacccgtag
     6901 cgtcagtgag atgcgccaga taccgccaaa cggcttccag gaaaaagcac ttaacttcct
     6961 gacgccgcac cagaaatggg gcattcactc aacctacagt gaaaacctgc taatgctgac
     7021 gctctctcgc ggtggtcagc caacgtgtac cgccgggcat gaccatgatg tatcacgccc
     7081 aggaacgcat tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata
     7141 actcggtcac ccgcgtttgc ccgaaaccga cgcatatgat tggcggttac gcgcagctgg
     7201 cctggggctt taactactac ggcaccgtcg gctcgaaccg cgacgaattc atcatgatcc
     7261 gcaagatgaa gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga
     7321 aaaaatgaaa atacgttcgc aagtcggcat ggtgcttaac ctcgataaat gtatcggctg
     7381 ccatacctgt tcggtgacct gtaaaaacgt ctggaccgga cgcgaaggca tggagtacgc
     7441 atggtttaac aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca
     7501 ggaagagtgg caaggcggct gggtccgcga tgtgaatggc aagatccgtc cgcgtctggg
     7561 cagcaagatg ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agcagattga
     7621 tgattactac gaacctttca ccttcgacta cgaacatttg catagcacac cggaaggcaa
     7681 acatatccct actgctcgcc cgcgttcact gattgacggc aaacggatgg acaaagtgat
     7741 ctggggccca aactgggaag aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg
     7801 caacttcgag gccatgcaaa aggagatgta cgggcagttt gaaaacacct tcatgatgta
     7861 cctgccgcgc ctgtgcgaac actgcctcaa tcccagctgc gtggcgacct gcccaagcgg
     7921 cgctatctac aaacgcgaag aagacggcat tgtgctgatt gatcaggata aatgccgtgg
     7981 ctggcgtttg tgcattagcg gttgtccgta caaaaaaatc tacttcaact ggaaaagcgg
     8041 caagtcagaa aaatgtatct tctgttatcc gcgaattgag tcaggccaac ccaccgtgtg
     8101 ctcagaaacc tgcgtgggtc gcatccggta tctgggcgtg ctgctttacg acgccgaccg
     8161 catcgaggaa gcggcgagca ccgagcgcga agttgacctc tatcgagtag gcagcctgac
     8221 ggctgcgg
//
LOCUS       DADMGC010000157         8193 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.157, whole genome shotgun sequence.
ACCESSION   DADMGC010000157 DADMGC010000000
VERSION     DADMGC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.157"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..671)
                     /gene="chbR"
                     /locus_tag="JNZ73_003611"
     CDS             complement(<1..671)
                     /gene="chbR"
                     /locus_tag="JNZ73_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HAY8733742.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTDSISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIIN"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="JNZ73_003612"
                     /pseudo
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="JNZ73_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
     gene            complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="JNZ73_003613"
                     /pseudo
     CDS             complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="JNZ73_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="JNZ73_003614"
     CDS             complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="JNZ73_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HAY8733743.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="JNZ73_003615"
     CDS             complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="JNZ73_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HAY8733744.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3680..4507
                     /gene="nadE"
                     /locus_tag="JNZ73_003616"
     CDS             3680..4507
                     /gene="nadE"
                     /locus_tag="JNZ73_003616"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAY8733745.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINLLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4737..5624
                     /gene="cho"
                     /locus_tag="JNZ73_003617"
     CDS             4737..5624
                     /gene="cho"
                     /locus_tag="JNZ73_003617"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HAY8733746.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5584..6159)
                     /gene="ves"
                     /locus_tag="JNZ73_003618"
     CDS             complement(5584..6159)
                     /gene="ves"
                     /locus_tag="JNZ73_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HAY8733747.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREISTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6362..6847)
                     /gene="spy"
                     /locus_tag="JNZ73_003619"
     CDS             complement(6362..6847)
                     /gene="spy"
                     /locus_tag="JNZ73_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HAY8733748.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7177..8145)
                     /gene="astE"
                     /locus_tag="JNZ73_003620"
     CDS             complement(7177..8145)
                     /gene="astE"
                     /locus_tag="JNZ73_003620"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HAY8733749.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHSEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         2152 a         2054 c         1892 g         2095 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactgcg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtcc gctgatacta tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttaca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaac gacccctccc caaagcttag
     2281 aaaaacgttg ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat
     2341 aaagccattt ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag
     2401 gagtaccttt tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca
     2461 atttaaatat tgcggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa
     2521 ttaatttgct gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc
     2581 gactttgcca taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg
     2641 gatttcgggc aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc
     2701 tttttcacca gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc
     2761 tgcctgtgcg cgcatttttg ataccagtaa agagatagac atgcccgcag aacaaaacag
     2821 ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac
     2881 tccgcaagcc ttaacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt
     2941 ttgtggctga aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa
     3001 attgtttctc cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga
     3061 agataattaa gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc gttgttactc
     3121 aagctttcag acgaattgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg
     3181 tctgataacc ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg
     3241 ctttaccatc acgttgaccc aggatgtagg tctggcaagt accgcgagca tggatcatgc
     3301 tcacttcaga cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt
     3361 tgacgtcttt caccacaggc tgtacaaact ggtctttggt acgatcataa gctgtacaac
     3421 ccgccagcat ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat
     3481 tccgtcctct tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa
     3541 gcccgtgaac gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt
     3601 cagcccgctg tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa gttttgtctt
     3661 ttctgtctgg aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa
     3721 accgcagatt aatgctgaag aggaaattcg tcgtagtgtc gattttctga aaagctacct
     3781 gcaaacttat ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac
     3841 gcttgccgga aagctgtgcc agatggcgat taatgagctg cgccaggaaa ctggcaacga
     3901 atcactgcaa tttattgccg tacgcctgcc ctatggtgtt caggctgacg aacaagattg
     3961 ccaggatgcc attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc
     4021 ggtattggct agcgagcagg cattgcggga agcaggcatt gaactgagcg attttgtccg
     4081 tggcaatgaa aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag
     4141 cggtgtcgtg gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata
     4201 tggtgacggc ggtacggata ttaatctgct gtatcgtctc aacaaacgcc agggtaaaca
     4261 gttactgacg gcattaggtt gcccggaaca cctttataag aaagccccaa ctgccgatct
     4321 tgaagatgat cgcccttctc tgccagatga agtggcactc ggcgtgacct atgacaatat
     4381 cgacgactat ctggaaggga aaaacctacc tgaacaggtc gccagaacaa tagagaactg
     4441 gtatctgaaa accgaacata aacgccgtcc gccaattaac gttttcgatg atttctggaa
     4501 aaagtaataa tttgcaccta ttggattgtg cgaaaaagag taatttgttc acgccggatg
     4561 cggcgtgaac gccttattcg acctacaaaa ctatgcaaat tcaatacatt gcaggagtcg
     4621 aataggcctg acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa cccgcctctt
     4681 ttttaatcgc ctcccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg
     4741 tacgccgttt aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg
     4801 aacatttaca ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg
     4861 gcgaaagtga caccatgccg ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc
     4921 tttctcattt acgcaccccg gatgaagccg ccatgctacg gcaatcccgg cgaattagct
     4981 ggatctgcac cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac
     5041 aacagccgct gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa
     5101 atgaaaagcg cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc
     5161 tgtttggcct gtttgccaat aggcgcgcag ctttgcaagc attgcagagc atcgctgatg
     5221 aacaaaaact ttgttatggc ctgttgggac tggaaccgtt aagtcgcggt cgtgcatgtt
     5281 ttcgttcagc gctaaaacgt tgcgccggag catgctgcgg taaagagagc catgaggaac
     5341 atgcgctacg cttgcgccag tctctggagc gtttgcgggt ggtgtgttgg ccttggcaag
     5401 gtgcggtggc actgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact
     5461 ggctgtggct gggggcggtt aattcgctgg aagaagcgac aacgttaatt cggacacccg
     5521 ccgggtttga tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaactatg
     5581 aaattactga acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag
     5641 tttaccttgc ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acgcgccctg
     5701 gtcggtagtg agcagtttgt cgcccagttg ccacgccccg ttaatcacaa acaccacccc
     5761 gccgcgtgag ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac
     5821 atccaggcgc gtcataatat tgaaatccat cgacatctgc cctgccgtca gtttcgcttt
     5881 taccacctgg tccgccgtaa aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg
     5941 gtctgcgctt tcaaggagca tctcgccgcc ttccagcaac gtcactatcc tttccatgcc
     6001 gggaaacaaa gaaaactccc cattcgccgc gatggatgca atgctggcac gccagtaaaa
     6061 atcacgtttt gccggtggga aagtggaaat ttcgcgcgtt tcaccggcag cgtttcgcca
     6121 cagattcacc gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt
     6181 ttcattgtta tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta
     6241 gtggtgatca ggcttttttt acaactgttt gagaagagaa aagaaaaccg ccgatcctgt
     6301 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag
     6361 attattcagc agttgcaggc attttacctt ttgccgctgg acgttctgtc agacgcttct
     6421 caaaattagc attaaattgc tttttctgtt ccggcgtcag gatgttgtaa attttgttct
     6481 gggtttccat gtgcgccagc atgttagctt tgcgctgttc ttccattttt gcgatctgcg
     6541 cttcagcttt tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt gcgcggcgtt
     6601 cttccagcgg cggacgtttc atctggtcac gctggccttt catgatttcg tggatctgct
     6661 gtttctgcgc gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc ggaccgaact
     6721 tgcctttgtg gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg gtgtctgcgg
     6781 catgggccag gttagccgcg ccaagagcca gggtagaggc aacaaacagt gcagttaatt
     6841 tacgcatatt ctatatcctt cctttcagtt atttattacg gctttcttaa gtagcgtgcc
     6901 gtgttgacga gattaacttt actgacttta gcgtcaatta atcagagcaa cggtaaaaca
     6961 atgaaagtgt aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa tttgatggag
     7021 agtgatgaga gaatattaca aaacgatgat tttgcagaga ttatgaagaa ctataccgga
     7081 tgactggtga taaataaagc aaataaccag gattaatctg tattaattta taagaaagca
     7141 acttaatacc cgcagaatga tttctgcggg taagtattag cttatttttt cgagcattaa
     7201 tcccgcgcgt aatcccaacg ctaccaacgg attagggaat aacacatact ctacatcatg
     7261 ggttacggta aaacgttcct ctccgtcctg cgccagcaat gttcctttct caaacggcat
     7321 aaaattcagc gtgtcacttg ccatatgcat ttcgaaggac ggcgagtggc gagtaatttg
     7381 cgaaaccacc cgataacgga gcggcggtgt tctcacgata ccgacactct caccagatag
     7441 cagcgcagca attgcgctgg cagttactgc aaactggcga agatcgtttt gcccaaaggg
     7501 caacgctttg ccaagttcca acgtacaggc cagcgcgcca aaatgtctga cgctgaaatg
     7561 ggtaaacgta ccaccaggct cctgatggaa caccagcgcc tccagccccg ccgcacccag
     7621 ccacgtcaga aatttctcgt cccagggaat gtcgcgttgc ggtaatacac cgaactgcgg
     7681 atgcaaggag ccacgaattg cggtatgtag atcaaggtgc caacgcacag attctttgcc
     7741 ctggtcataa aaatcttcca ggcactgttc cagttcgcgc gcccgacagg tttctccgct
     7801 ttcagcaaat agctgccaac gaccgccaaa cattcggttc atatcgctat ggcaataacg
     7861 tttcccttgc ttcagcgcag gaggattccc gaggatcacc agcaaccgcc aacgtaacgg
     7921 gatctcgctg tgagatatcg cgccaagcaa cgcgtccagc atttccacag gtgccgtctc
     7981 attaccgtgg atccccgcag aaatcaccag cgcgccttgc ggtggcgtta atggcgtcag
     8041 ttccagcaca ccatcgccca gccagcgcca gcgaacgccg ttgatttctc gctcggtgat
     8101 aaccggtttt ttacccgtta aggtcagagc aagaaaatta tccattgccc cctccctcgc
     8161 gctggaacgg ataaaccgaa ccgagattca gta
//
LOCUS       DADMGC010000158         8157 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.158, whole genome shotgun sequence.
ACCESSION   DADMGC010000158 DADMGC010000000
VERSION     DADMGC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.158"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(61..600)
                     /locus_tag="JNZ73_003621"
     CDS             complement(61..600)
                     /locus_tag="JNZ73_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY8733750.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(665..1213)
                     /locus_tag="JNZ73_003622"
     CDS             complement(665..1213)
                     /locus_tag="JNZ73_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8733751.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTINFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(1694..2290)
                     /gene="fimE"
                     /locus_tag="JNZ73_003623"
     CDS             complement(1694..2290)
                     /gene="fimE"
                     /locus_tag="JNZ73_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimE"
                     /protein_id="HAY8733752.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(2768..3369)
                     /gene="fimB"
                     /locus_tag="JNZ73_003624"
                     /pseudo
     CDS             complement(2768..3369)
                     /gene="fimB"
                     /locus_tag="JNZ73_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
     gene            4823..5539
                     /gene="nanC"
                     /locus_tag="JNZ73_003625"
     CDS             4823..5539
                     /gene="nanC"
                     /locus_tag="JNZ73_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HAY8733753.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            5559..6665
                     /locus_tag="JNZ73_003626"
     CDS             5559..6665
                     /locus_tag="JNZ73_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HAY8733754.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNRWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKEEYYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            6730..7710
                     /locus_tag="JNZ73_003627"
     CDS             6730..7710
                     /locus_tag="JNZ73_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAY8733755.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2422 a         1743 c         1699 g         2293 t
ORIGIN      
        1 tacattgacg tttttattac tcactgtcct gttcctgtta tcacattatc tactgaacaa
       61 ttactgatag gttaaagaga accaggcctg ggcattggcg atgccgccag taacccgacg
      121 cccggtagca cgatatttgg cgatgaaatg tagcgaagta gagcctgagt aaagccgttt
      181 ccagtttgct ggaggacgat taatcggtac gaggtttcct tcatcatcaa acaacgctac
      241 accaatattg gaggctatcc ctggcccctc tcccacggag agcacatccg gatttttacc
      301 atccgcgaca ccgtgaaacg ccacacccac acgttcactc accaccgtgc tacattcccg
      361 taaatgaata acaaaaggca ccggtgcgct atcttcccct gccgcatgaa accggttact
      421 gctgatttgc cccatattga ccgtcatttg tttatcaccg gcttcaatcc ggcaagtttc
      481 cgcaataatg acgccctgaa attgcatatt tccgccgggc agcgtggtat tccatttatt
      541 tccggccaga gcaaacattg gcagcaacga cgctaacaga aataatctct tccttttcat
      601 cgttattttt atcgcacaag ggtgggcatc cctgcccgta atgacgtcct tgaacctggg
      661 taggttattg atactgaacc ttgaaggttg catccgcatt agctgcaccc ggggttgcct
      721 cgccgattgc ataataacgc gcctggaacg gaatggtgtt ggtaccgtta ttcagggttg
      781 tttgtgcact aaatgtcgca ccgtccagcg tcagagcatt gcctgttctg tccaggatct
      841 gcacgccaac gtttgttgca ctacctgcag cagaactctg cagagctagt acatcagtac
      901 gcgtagcatc aattgccgta cctaagaagg caacagcggc ttttgtggca acagtggtat
      961 cgcaatcatt cagctgaatg ttaaaaccaa cggcagagct ggttgctcca gcctgcttca
     1021 ggctagcggt acgaacctgt cccaactgaa cggtttgatc aacagagcct gcatcaactg
     1081 cgcaagcggc gttaacaact tcccctttaa agttaatggt cccaccattt accgtcgtag
     1141 tatcggccag agccgctgcg gaactgaggg acagagccga cagaacaacg attgccagag
     1201 ttttaatttt catgctgctt tcctttaaaa aaactatttc taaatcgaca tgggcagtcg
     1261 ttctgtacac tttgttttgt caacgagttt gactgccaac actgcacagt tttcccccaa
     1321 aagatgaaac atttggggcc attttgactc atagaggaaa gcatcgcgca caaacttttt
     1381 cagtttattt gttggcttaa tattctattg ttatctttat ttatagattt ttatattgca
     1441 tgaggtggtt tttggagaga agaatgagga agatgcgtcg agccacagaa acgttagctt
     1501 tacatatagc ggaggtgatg tgaaattaat ttacaagaga aataatttac atatcaaata
     1561 gttagatgct ttttgtcgtt ttttaatatt tttatgcttg agaaaaaata cgtaacttat
     1621 ttatgatatg gacagtttgg ccccaattgt cttgtgttta tttgtttttt aactttatta
     1681 tcaataagtt aaatcaaacc tcttctcttt ttaatttttc gtttatgaga ttatttcttt
     1741 cccataatcc ggcaaaacga gcagcattac tggcggtata acgcacagta tggcgaatat
     1801 ttcgatgccc gagataatcc tgaattaaac gggtatctgc accacgctcc gccagttcat
     1861 aaccgcaagc atgccttaac atatgaggat gagtctgcgt tacggttcca gcttcaatac
     1921 cggcatcgcg aataatgcga taggcctgct ggcgagaaag ccgactcccg cggcgagaaa
     1981 taaatatagc gtcggtccgg tcagcgcctt tccagttagc acgttcctgg gtccagcgtt
     2041 ccacggcttc acgctcatca aaacgtaacg ggtgaacggt agaaaatccg ttcttcagtc
     2101 ggcgaatatt tattcgacct tcattaaggt caaggtcctg ataatgcaga tcaagcagtt
     2161 cactaatacg catcccatgc cgatatgcca acagaataag acaataatct ctggctcccg
     2221 ttgccccgta acaaaccgcc tgcatcatgg cctgaacttc tttaccggta agataacgac
     2281 gtttactcac aatagtagta ctcctaactg agatatattt gaatgaatac ctataggaaa
     2341 cctcaatcgg tcaaaattag cccaatagat aaaaagataa ccacacacga agttatgtgg
     2401 ctatcagtca attacattaa acatcataca aaaataaaat atcactgatg tgacaacatc
     2461 tattttcatt gatttgattc aatataacaa tagattacag aggattaact tccagttact
     2521 taaaaaataa agaaaaaagt gtggaaacag gagaatttaa gtgatgtgta aacttatgaa
     2581 tgattttgtt tatttatttt gaagaacata aaccattaga ttttgtatgg gtatggttaa
     2641 cttaatgttt attatgataa ttaagaatca cacttacaat aacatcaatt aataaatggt
     2701 taatcatttt tgatatatcg taagaataat gtagttttta acaccatccc tggtatctca
     2761 actatctcta taaaacagca tgacgctgtc gtcctctggc tctatcccag atgccgtaaa
     2821 aacgccctgc attgctggcg gtataccaga cagtatgacg aatattgcga tgcccaagat
     2881 aatcctagat aagtcgcgta tctattccta tattcgccaa agcaaaacca cacgaatagc
     2941 gtaacatgtg cggatgaatc tccagtgaca acccggcatt accaccagaa gtcgagataa
     3001 tatggtaaaa ctgttgccga gaaagcggat tccccttacg tgataaaaat acccactcgc
     3061 tctcagcatg cggataagaa gtacggatac tcaaccagtt ttttaaagcc tgaacttctt
     3121 tgttcaatag cgggtgcgtt gttgaaaagc ctttttttaa tcgatggata tatatacact
     3181 ttgctttaag atcaatatcc gaaatcctca atcgacaaat ttcactcgcc cggaaaccat
     3241 gaataaaaca aagcaaaatc agacaataat tacgtgttgc atgaggccgg tatttgctgc
     3301 tttaaggagt gactcgattt cactatgggt aaggaagttc ctttttttgt tatcagcctt
     3361 attcctcatc gttttccctt ataattacag acgcgcacta gctgtgctgg gttagcaata
     3421 atccaacatt tttatcctga ttatgtttat aaaagcgaac gtttgcttaa taacttacgt
     3481 aagtggacca gttcttctga gcgaacttaa atggagtagc gatagttact tataataact
     3541 ttgccataca aacgccatgc tcttgcatgc tatgtacctt tatatattta tcaatctgat
     3601 acgaatctaa taataaccct catcattaat gcaactaatc ttatctatat atcatgtgat
     3661 atgttctgta acaagtaaaa tcaacataaa aaattcagca aaatttaata tttgttcaac
     3721 tagccgtctc aaatatgaca ttattagtta actgcatttg ccacaaaatg gctatttcat
     3781 ggaacgcagt aatttcttag tggctattat catgctaatt cttagatccc tagcgattat
     3841 tcctgcctgg ttcgtcagct taggccatga gattgttaca ttccttttta tcctggagat
     3901 atgggggaac gcaggcgacg ctttttcatc tacatccgtt ggtaaatata gctttggtca
     3961 ctacgactta agccttcctc aatcacagct attcgccggg cgatacggca aacaaactaa
     4021 gtgaaacatc atcctgaaaa caccaacatc aacaagcctc tccagatcga catcaggagt
     4081 gaccagttac aagccacaaa ccaagaaaca tatttcatga tgagaattat gctcagcctg
     4141 atggcgagta aacccttatt ttcaccactc tcactgagtt agcttgcaaa tctttctttg
     4201 tttagattct aaataaattt tttataaaac atatagttaa aagaaaatct ttagtttttg
     4261 aagaccaccg caagtgttcg tctggcttca tatggcatct tcttcttaga aaaagatcga
     4321 catattttgt gacgcgaatt gcaaatctgg ttttgttgta tggattgcgt gatttttgat
     4381 ctggtataac aggtataaag atgcaccacg atagtcaatg agactgggca tctcgtaatc
     4441 tatggcaaac atcacttcag ttctttcaca tcgggtgatg aaaatgcact tcagtctgaa
     4501 aggaatatga aaatgagatc aacagacact ctattttatg actctgggta aaatgggttg
     4561 agtaagtgat ataacttacg aatattcaaa tcaattaaaa atcagaagag attttatact
     4621 caggtattta atctggatct ctgtttattt aaataatgtt aaaagagaat tttcacagga
     4681 gaccttatac aaaaaatata aaatacagtt acccgttgcc aaagacacta taagcctggc
     4741 aaaaaaatat tacacaacat aaatgctaat tgtttatgcg ggctttgtat tgctttctgt
     4801 atcctacaaa tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg tattattact
     4861 gtgcttttcg tccccattaa tttctcaggc tgcgacactg gacgtacgtg gtggatatcg
     4921 tagtggaagc cacgcctatg agactcgact caaagtcagt gagggatggc aaaatggatg
     4981 gtgggcaagc atggaaagta atacctggaa taccattcat gataataaaa aggaaaatgc
     5041 cgcactcaat gatgttcagg ttgaagttaa ttacgcaatt aaacttgatg atcaatggac
     5101 ggtgcgcccg ggaatgttaa cgcattttag cagcaacggc acacgctacg gaccctacgt
     5161 aaaactgtcc tgggacgcga caaaagatct taattttggc attcgctatc gttacgactg
     5221 gaaagcttac cgacaacaag acttatccgg tgatatgtct cgtgataacg ttcatcgttg
     5281 ggatggatat gtcacttacc atattaatag tgatttcacc ttcgcatggc aaacgacgct
     5341 atacagcaaa cagaatgatt atcgctatgc aaaccacaag aaatgggcga cggaaaatgc
     5401 ctttgttcta caataccata tgacgcccga tattacgcca tacatagaat atgactacct
     5461 tgaccgccag ggtgtttaca acggcagaga taatttatcg gaaaacagtt atcgcattgg
     5521 tgtgtcattt aaactgtagt agacaggaga cagtcacaat gaataaaact ataatggcgc
     5581 ttgctatcat gatggcttca tttgccgcaa acgcgtctgt attaccggaa actcctgtgc
     5641 catttaaaag tggtaccgga gcaattgata acgacactgt ctacattggt ttaggtagcg
     5701 caggtacagc atggtacaag ctagatacac aggccaaaga taaaaaatgg actgcgttag
     5761 ctgcattccc tggtggaccg agagagcaag caacctctgc atttattgat ggcaatctgt
     5821 atgtgtttgg cggcattggc aaaaacagcg agggattgac tcaggtattt aatgacgtac
     5881 acaaatacaa ccccaaaacc aatcgctggg ttaaattgat gtcgcacgcg ccgatgggca
     5941 tggcgggtca tgtgactttt gtacacaacg gcaaggctta tgttactggc ggtgttaacc
     6001 agaatatctt caatggctat tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta
     6061 tagataaaat caacgcccac tattttgaca aaaaagaaga atattatttc ttcaataagt
     6121 ttctgttgtc ttttgatccc tcaacacagc aatggagtta cgctggcgaa tcgccctggt
     6181 acggaacggc tggtgcggcg gttgtgaata aaggtgataa aacctggctt attaatggcg
     6241 aagccaaacc aggattgcga acggatgccg tatttgaact tgatttcacc ggtaataatt
     6301 taaaatggaa taagcttgat cccgtctcat caccagatgg cgtcgctggc ggttttgcgg
     6361 ggataagcaa tgattctctt atatttgccg gaggggccgg attcaaaggt tcacgagaaa
     6421 attaccagaa cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat agcactgata
     6481 ttcatctttg gcataacggg aaatgggata aatcgggtga attatcgcaa ggtcgggcct
     6541 acggagtatc attgccctgg aataatagtt tattaattat tggcggtgaa actgcaggcg
     6601 gcaaagcggt gacggattca gttttaattt ctgtgaagga taataaagtc acagtacaaa
     6661 actaacgctt cagggccccg gtaaaggggc cctgctatca atttgtcatt tgtattacgc
     6721 gaaaattata tgaacgcaat aatatcgccc gattattact atgttcttac cgttgctggt
     6781 cagtctaatg ccatggcgta tggcgaagga ctgccattac cggacaggga agatgcgcct
     6841 cattccagaa ttaaacaatt agcgagattt gcgcatacgc atcccggagg cccgccatgt
     6901 cactttaacg acattattcc actgactcac tgcccacacg atgttcagga tatgcagggt
     6961 tatcaccatc ctctggcaac gaatcatcaa acacagtacg gcaccgttgg ccaggcactg
     7021 catattgcac ggaaattact gcccttcatt cctgataatg cagggattct tatcgttccg
     7081 tgttgccgtg gcggatcggc ttttaccgcg ggcagcgaag ggacatattc agaacggcac
     7141 ggagccagcc atgatgcttg tcgttgggga acggatactc cgctgtacca ggatttagtc
     7201 agcagaacgc gagccgcact ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg
     7261 atgcaaggcg aatttgactt aatgaccagt gactacgcgt cacaccctca acactttaat
     7321 catatggttg aagcctttcg tagggatcta aaacaatacc attctcagct taataatatt
     7381 actgacgcgc cgtggttttg cggcgatacc acctggtact ggaaagaaaa tttcccgcat
     7441 tcgtatgaag ctatttatgg caattatcaa aataatgttt tagccaatat tattttcgtc
     7501 gacttccagc aacaaggtga aagaggactg acgaacgcgc ctgatgaaga cccggacgat
     7561 ttaagcacgg gatattacgg ttcagcgtac cggtcaccgg agaactggac gacggcactg
     7621 cgaagcagtc atttcagcac ggcagcccgt cgggggatta tttctgacag atttgtagaa
     7681 gcaattttgc agttttggcg cgaaaggtga gcggggagtt tattcatggg ggatgtcctt
     7741 gttcgttctc agagtagaca acaaaaaaca aatgagatca aggtaatatc tcttcacttg
     7801 cgggctcgaa cagcaggcaa ttgccaatat aataatctgg cacaagggtg aaaaacgcgc
     7861 accgcataaa ccgttgttat tgcttacaga ttagccggat gccttaatag acatccgcgc
     7921 cttttcgatt atggctcgga aatctacgaa catctacaca gtttactgga acgttttggg
     7981 ccacagcgtt cgcagcaccg tcctgatatg ccgttctggc gattataagt cgacggattc
     8041 tggcaaatag acgatgatga tatgcttaat gatgttcaaa gaaattaggc cattattata
     8101 ttattgcttg cattttcttt cattcctaaa agcacccgcc attaatccca ggcgcat
//
LOCUS       DADMGC010000159         8142 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.159, whole genome shotgun sequence.
ACCESSION   DADMGC010000159 DADMGC010000000
VERSION     DADMGC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.159"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..893)
                     /locus_tag="JNZ73_003628"
     CDS             complement(<1..893)
                     /locus_tag="JNZ73_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAY8733756.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPS
                     SVNVLSELPGRTVPYEAAEIRPQVGGIIIKRNFIEGDKVNQSDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSR"
     gene            953..1231
                     /locus_tag="JNZ73_003629"
     CDS             953..1231
                     /locus_tag="JNZ73_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733757.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1232..1759)
                     /gene="gadE"
                     /locus_tag="JNZ73_003630"
     CDS             complement(1232..1759)
                     /gene="gadE"
                     /locus_tag="JNZ73_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HAY8733758.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2558..3130)
                     /gene="hdeD"
                     /locus_tag="JNZ73_003631"
     CDS             complement(2558..3130)
                     /gene="hdeD"
                     /locus_tag="JNZ73_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HAY8733759.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3385..3717
                     /gene="hdeA"
                     /locus_tag="JNZ73_003632"
     CDS             3385..3717
                     /gene="hdeA"
                     /locus_tag="JNZ73_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HAY8733760.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3833..4159
                     /gene="hdeB"
                     /locus_tag="JNZ73_003633"
     CDS             3833..4159
                     /gene="hdeB"
                     /locus_tag="JNZ73_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HAY8733761.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4223..4869
                     /locus_tag="JNZ73_003634"
                     /pseudo
     CDS             4223..4869
                     /locus_tag="JNZ73_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4911..5441)
                     /locus_tag="JNZ73_003635"
     CDS             complement(4911..5441)
                     /locus_tag="JNZ73_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8733762.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5597..6163)
                     /gene="slp"
                     /locus_tag="JNZ73_003636"
     CDS             complement(5597..6163)
                     /gene="slp"
                     /locus_tag="JNZ73_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HAY8733763.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGLGAPYYTNAVSQVTPELVK"
     gene            complement(6410..7633)
                     /locus_tag="JNZ73_003637"
     CDS             complement(6410..7633)
                     /locus_tag="JNZ73_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733764.1"
                     /translation="MSIDFTLGIINTYHGDIYNCTTNTDNVKTPDTPKWPYDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
BASE COUNT         2366 a         1574 c         1702 g         2500 t
ORIGIN      
        1 cggctacctt catccactaa tgccgtgaca tacatgccag gcagcaagtc accatttggg
       61 ttggggaaaa tcgcccgtaa cgtcacggag cccgtggttt catcaaccgt cgggtcggag
      121 aatttcagcg tgccggtctg gctgtaacgt ttaccatttt ccagattgag ctgtactggc
      181 gtactgccct gaacctgttt gatttgccca ctggcgacct cttctttcat gcgtaagaaa
      241 tcttgcaccg actgcgtgag atcgacataa atcgggtcca gacgttgtac ggtaaccagc
      301 gaatctgcct gattagcggt aacgagtgcg ccgacggtca ccgacgattt cccgctgacg
      361 cccgtaatcg gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg ttcaacagcc
      421 gctttggcga cggtgacatt ggcttctgct tcattcagct gggtgcgcgc ggtgtcgtaa
      481 tcctgacggc taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa ggtgatgcgg
      541 gcattgctgg cggtagagag cgctttcgcc agcgagcctt tggcggagtt tagctcggcc
      601 tgtaaaggtg caggatcaat ctgatacagc gaatcgctct ggttcacttt atcgccttcg
      661 ataaagttgc gtttaatgat aataccgccc acctggggac gtatctcggc agcttcataa
      721 ggaacggttc taccgggcaa ttcgctcaac acattgaccg aactagggga gagtgtgacg
      781 acaccgacct caggcgtcat ggcggcggcg ttttccgccg atttgtcatc gcaggcggtg
      841 agcatcgcgc cgcagaataa caacggtatt aacagctttc ttcttctgtt cattttagtc
      901 cctgaaaatt cttgagaaat aacggttctt taaattgcgc gcgaggagag atatgaggga
      961 tttacagacc agcggcatag tggggttgtc agccagtaag gtgggataca ggcacagtga
     1021 tcgacatggt gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag ttcctgccag
     1081 cattcgggca acgaagggtt ctactggtgg atacacatac caggggaatc aagtcgtttt
     1141 agcctctcct ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc ctcgtcatgc
     1201 cagccatcaa tttcagttgc ttatgtcctg actaaaaata agatgtgata cccagggtga
     1261 cgatgtcgct catacgtctt agtttcagct tgtacataat gttgaatttc tgaatcttta
     1321 atgtttgttg cgaaatattg agcgtcgacg tgatattgct ttcattttta tttttcagaa
     1381 tcagttgcat aattttgaac tgattatgtg aaaaacataa ttgtgaattc ttatggggca
     1441 agtgtttacc ataagtaatc tcaagaagat tttttatact ttctttgcgg ctaacaaaat
     1501 aaataggcgc tttagctttt agtttactga tgtgataagg tgccaaaaca ataattctct
     1561 cggcatctaa tttctccaga aatttaatcg cttcttcatc aaggatatga ttgtgatagg
     1621 tatcaatgat aaccttgaag ggtttattgc ctactttttt gatctctgac agggaattga
     1681 tttttatcat ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga aaagaatctt
     1741 tcgtcatgag aaaaatcata acttgctcct tagccgttat tgtttcgaac atgtcatcct
     1801 tttcattatt tacatccttg tccgaatcgt tgttcaatat agtaaacgcc tataagaact
     1861 ttgcccttcg tgaactaata cgtctattaa cactaatcaa tacttcagat ttatccatca
     1921 cgcactccat atattgatcc taagtaagat tcctggttgt tatcagcttg taaatagtaa
     1981 acaaaaagtt aacgtttgcg gattactgcc caagaataag ttatcaataa ccagtttgtg
     2041 atctctgaag aatattacta aagttaaaat gtaatccgat ttaaatatcg agtctccttg
     2101 tttcgactta agctggcaat tggattgcca gctttctttt aacgcattga aattacctga
     2161 gtaaataacg catttatgtc ttcaggtttt tatatagcgt ctggagacac ggatatttat
     2221 gcaatgtaat caacgccagt tgcaaaaaaa tgaacccgga ttacttattt aaccgcccat
     2281 agcatcaacg cttacggaac tctttgttca ctttcccaca gcacttttaa aacattaaac
     2341 ctgactacgg aaaatatcag ccatgataat gtttgaaatg gctaatttgc catggagtga
     2401 aaaaaattag atgaaattca gtaggttgaa ataatcacta gcaggtaatt atttcaatga
     2461 tagtgcgcaa ttgatctaca acactgcgta gcggagagag tattaatcgg atcatagtca
     2521 catcaagtga ctatgatccg ggtgacaacc ggggtaatta ttgctgctta acgaacaaac
     2581 tggcgaagct gaacaggctg gcggcgctaa atatcagttc aattcccacc agtgtggaaa
     2641 ccagcgttac agacaccatc ggcgttgcac caaggaatat ccaggcaatg acgatatcca
     2701 gcacaccaat aacgagctgt agccagctgc ctttcattga acgctgacga taccaactca
     2761 tcaggcgaat aacccctgca acacagaaca aaccggcaat aaatgccgca atggcaaaaa
     2821 tgcccagctc cggtgcacgg atgaagaaat agccgatcaa taaataggcg actgcgacga
     2881 ggaaaccgga taataccggc cagaaattat gactgcggtt gctgaataac ccgacaataa
     2941 gcgcaatacc cgagcagatt aataatgcac ccactactgt gcttaaaata tcgccagaga
     3001 cgaacgggaa actgatacac agcaacccga cgataaacag cagcacggca ataaactgga
     3061 ttgctctgcg atgctttttc agcatctcca gatcaaactt caaaattgtt gccttatcta
     3121 tatataacat agaaccaccc tataaaatta agaagaaaat cccctgctat caatctatgc
     3181 caaaaacgcg tctaagaatg cagtcgattt aataaaaatt tcctaattgc agtatctgat
     3241 gcatctgtaa ctcattgtat tgaaataaaa atatctgatt ttgatatttt ccatcaacat
     3301 gacatataca gaaaactagg ttataacctc agtgtcgaaa ttgattcgtg acggctcttt
     3361 cactttatag ttgaggatat tacgatgaaa aaagtattag gcgttattct tggtggtctg
     3421 cttcttctgc cagttgtgag caatgcagcg gatgcgcaaa aagcagctga taacaaaaaa
     3481 ccggtcaact catggacctg tgaagatttc ctggctgtgg acgaatcctt ccagccaact
     3541 gcagttggtt ttgctgaagc gctgaacaac aaagataaac cagaagatgc ggttttagat
     3601 gttcagggta ttgcaaccgt aaccccagct atcgttcagg cttgtactca ggataaacaa
     3661 gccaacttta aagataaagt taaaggcgaa tgggacaaaa ttaagaaaga tatgtaattc
     3721 cgggaatgcg ttacatcgta cttccttgca tattgaacag gccggaatat cttctttaaa
     3781 agcagctatt cctcctgttc atatataatc tctatattga atgggttaca aaatgaatat
     3841 ttcatctctc cgtaaagcgt ttatttttat gggcgctgta gcggctttgt cactggtgaa
     3901 cgcacaatct gcgctggcag ccaatgaatc cgctaaagat atgacctgcc aggaatttat
     3961 tgatctgaat ccaaaagcaa tgaccccggt tgcatggtgg atgctgcatg aagaaacagt
     4021 atataaaggt ggcgataccg ttactttaaa tgaaaccgat ctcactcaaa ttcctaaagt
     4081 gatcgaatac tgtaagaaaa acccgcagaa aaatttgtat accttcaaaa atcaagcatc
     4141 taatgacttg ccgaattaat gaggtacaag taaaaaggag tagcaagttg agccatcttg
     4201 ctgctccttt ttgcattttt atatgacagc agaattaatt atacgtctta tactcgcggc
     4261 aattgcctgt ggcgctattg gcatggaaag gcaaatgcgc ggcaaaggag cagggttacg
     4321 cacacatgta ttaattggca tgggaagcgc cctgtttatg attgtttcga aatatggttt
     4381 tgctgacgtg ctgtctttag atcacgtcgg actcgacccc agccgtattg ctgctcaggt
     4441 ggtgacgggc gtcgggttta tcggtgcagg taacattctc gttcgtaacc aaaatattgt
     4501 aggtctgacg acggcagcgg atatctgggt gaccgccgca taggtatggt tattggcagc
     4561 ggtatgtacg aactgggtat ttatggttca gtgatgacct tgttggtgct ggaagtcttc
     4621 catcaattaa ccttccgcct gatgaacaaa aattaccatt tacagctgac attagtgaac
     4681 ggtaatacgg tatcaatgct cgactggttt aagcagcaaa aaataaaaac tgatttagtt
     4741 tcgttgcagg aaaatgaaga tcatgaagtg gtcgccattg atatccagct tcatgccaca
     4801 acgttggtag aagacctgtt gcgattatta aaaggtatgg caggggtgaa aggcgtttca
     4861 attagctaat ctgtgtggca gcgtagccag actcaccgta agcctgaaat tcacaccaga
     4921 taatcaatat gctgatggcg taccagctca ttgatccgtt taagattgag gatcatcatg
     4981 atattgtgtt tatggcagta gaacgttttt agcgatattt tcagtttctc cagaatatct
     5041 tcagttgtca tttgattcgc aatgcagctc agaacctgat gttgcttgcg cgaaagtgac
     5101 acattcgctt caggcacaga gcagtaactg ccattgatga gtgcgacaaa atcaatgata
     5161 gtgatagtcc gcggaacaaa ggtcaccggt actttcccca gacagcgttt aatattaaag
     5221 ggggagaaaa caatgacatg gacaggtttt aatcgttcaa tttgagtgag aaaattagaa
     5281 tgaaatacat tattcattaa tgtatcaata atgacgaagg cattctgatg caacattacg
     5341 tcgatctctt cttcgttctg tatatgaacg acattacctt tactcagaat gtttttcatc
     5401 gcggtgaaga acatcgtatc cctggtaatt ataagaaaca taaccgactc ctggtgcgga
     5461 cattaacttt ataatcagtt tgccaaaggt aattaacagg acagggctta taataataat
     5521 agttagctta ctgtatatta attttatgta atacatgatg cagatgctga gaaagcatct
     5581 gcatctttcg gtggtgttat ttgaccagct caggtgttac ctgactcacc gcattggtgt
     5641 agtaaggcgc acccaagccc ggttccggcc agaatgcacc atagccgtaa tcccacaggt
     5701 tataggtggt attaaccact tctctcaaat gccacacctg gatgccctgc atattcactt
     5761 ccaggaagtt atacgggact ttattgataa agcctggttg ttcaccctga atggtaccga
     5821 ggatggtaac aaagtgatta cgatagttca ctggatcaag gaagccgctt tgtctggcga
     5881 gcagtcggcc ctgatagttg gcttcaatat caggcttcgc atagctatcc aacggtaata
     5941 cagcgatttc taacaacgta tccgttttgc cattgataac gttgataacc ttcccaccaa
     6001 agcgcgcttg ttgaccaaca tataaccccg gctggttatg aacagcaaca aaactttttt
     6061 gaatatcagg ttggttattg cctttgatat tttgcggaat tgaactacat gcggcaagca
     6121 aaaatgaaag gctgaggatg agtgcacctt ttgtcatgtt catgttacta tccttatcaa
     6181 caaatattaa aaccataata gatatttctt tatagttttc atctgattct gaggtgatat
     6241 gcctgatagt aacgtaaacc tgtctgatgt ttatatccct tctgtttagt tcaattccgt
     6301 tattttgggg cgtggtatga gtatccatcg cccaataata taaccctttg ttttaaaatt
     6361 acataaaatc tatttttatt caatgattat aaagtgctaa taatcaatat tatggtatta
     6421 tattctgata atgatttgat aagcgaattc ttttgccaga tggtatttct atttccaccc
     6481 cataatttat atctttttca ttaggcagat ctctctttaa ataaaagggg tatgaaatct
     6541 tttcgggttg aagtgtctcg taatgaaatg ctatgaattc atttttagac attgttgccc
     6601 atttttgaat tttattgtat agagagggtg ttgtaaactc ttgaagttgt ttataacata
     6661 tttttttgaa ctcttgctta tattttaata tgtcatcaaa tgaaagattc actacctttg
     6721 cctctaagaa atcaattatt ctcgaagcaa tttccgcttt cgcgcgtaaa gagttgtcat
     6781 taatagttaa caatgtagaa aacaagccat tgatttgttt gtcaagatgt tgttcatatt
     6841 tatgtagttt ggggatggga ttttccttgt gtgaaaaaat agtgttaaat aaatttgcgt
     6901 cagtgaaatt tatattaagt agatcttcaa aataaaattt tgataaggtt aggactagct
     6961 tggacatatc ttgccctgtc atgttgaaat caataaagtc gcatccagca agggcttcca
     7021 tcctattttc atttttactg tgctcaatat acagcttcgc catttttaag tacaagcttt
     7081 gaatgtctct tatatcggtt ggtaagtcag ctcgttgccc agcaaaattt ttattatcgg
     7141 aagaaacggt ttggacactg aatctttcgg gtattccttc ttctacgaga attttagtta
     7201 tgattggatt gttgggttct ggattaacga caacaatgat ttgacataca gaatccttat
     7261 ccttttcgta tctaatgtta tgaatctgat taagtattga ttgtgtctgt tgcgtaagtg
     7321 gtgtcgaact tatcgtttca atgttattaa taagacttac aagttgaacc acaacttttg
     7381 agtagtctgc atttgtatat gtggtgttcg tgtggcatgc attaatttgt tggagttgtt
     7441 gttgcgctaa atcaaattgt tcagggttgt atccttctcc agagaatgtg gaattaatgg
     7501 gttgttgttc ttcccaatta tcataaggcc acttcggtgt gtctggcgtt ttaacattat
     7561 cggtatttgt tgtgcagtta taaatatcgc cgtgatatgt atttattata cctagggtaa
     7621 agtcaattga cataacattc cttattcatc atctgtttta tgctaatgtt tttaaattgg
     7681 ataattagaa caggaatgga ttttcttcta cacacaataa atataatgcg ttatatcttc
     7741 ctgatataat tacctttaaa ttccctttca tttccacccg gtaatatttg tgtataaaaa
     7801 aagggcagtg atagtatcgt aaaggaaaac gttaacaact atcaaaaagc gctcatttta
     7861 aaaagttatt atgttctccg atagtgtgtg aagtaatatt tttgtgatgt tttagatgtg
     7921 taataatgaa ccaggttaaa gtgtatttaa aaatgtgttg tattagcgac tgaaatctgc
     7981 cttttaaaaa ggaggagggt actgagacaa agagtcattg cttctccgat aaaatgtcag
     8041 agaagcatga ttacgtggtt attgtctgtt gatgacttgt tgaccgagcc gtcatcattc
     8101 tcaatgcatt agttaaatca tttgtgattt ttaaggatta tc
//
LOCUS       DADMGC010000160         8079 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.160, whole genome shotgun sequence.
ACCESSION   DADMGC010000160 DADMGC010000000
VERSION     DADMGC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.160"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..1242)
                     /locus_tag="JNZ73_003638"
     CDS             complement(<1..1242)
                     /locus_tag="JNZ73_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAY8733765.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRNAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSL"
     gene            complement(1239..2117)
                     /locus_tag="JNZ73_003639"
     CDS             complement(1239..2117)
                     /locus_tag="JNZ73_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY8733766.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(2128..3035)
                     /locus_tag="JNZ73_003640"
                     /pseudo
     CDS             complement(2128..3035)
                     /locus_tag="JNZ73_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(3022..3255)
                     /locus_tag="JNZ73_003641"
     CDS             complement(3022..3255)
                     /locus_tag="JNZ73_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733767.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(3252..4109)
                     /locus_tag="JNZ73_003642"
     CDS             complement(3252..4109)
                     /locus_tag="JNZ73_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAY8733768.1"
                     /translation="MSAQYIDSAISGRYTVSAPYKAGAGIGVLKFNIEHNRAHAVFSC
                     HEHCYAQIMVGRAGASQDAPGSMLTGYANPVRLTTSVIGVPCGEFFEFNIGAVTMTTL
                     PTLSQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRILDCSPEFCARNFAETSILV
                     RQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMERSLSGAGAADM
                     SSVAQNARGVYLHLREIHQIWTSQLYPMLKAVESPLAGKLYDRVGDAVFGAALVDSRL
                     NGSDKEVLP"
     gene            4134..4463
                     /locus_tag="JNZ73_003643"
     CDS             4134..4463
                     /locus_tag="JNZ73_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY8733769.1"
                     /translation="MNYTIEYYSEEVRLEVDQLPLSMRARYQHLVERMKVYGSNLGEP
                     HTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKTQKTPPAERKKAETRM
                     KEVKHDW"
     gene            4453..4761
                     /locus_tag="JNZ73_003644"
     CDS             4453..4761
                     /locus_tag="JNZ73_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005286394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8733770.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(4803..4979)
                     /locus_tag="JNZ73_003645"
     CDS             complement(4803..4979)
                     /locus_tag="JNZ73_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HAY8733771.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(4976..5386)
                     /locus_tag="JNZ73_003646"
     CDS             complement(4976..5386)
                     /locus_tag="JNZ73_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HAY8733772.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(5383..5661)
                     /locus_tag="JNZ73_003647"
     CDS             complement(5383..5661)
                     /locus_tag="JNZ73_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733773.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQAPRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(5736..>8079)
                     /locus_tag="JNZ73_003648"
     CDS             complement(5736..>8079)
                     /locus_tag="JNZ73_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HAY8733774.1"
                     /translation="GDCEPCLFGWQALDAGVRAVVLCEGEIDCMSYAQYGISALSVPF
                     GGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASRLGEHRCRLVTLPYK
                     DINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAFYGKQQYLFNPPWES
                     LADKFQFREAELTLVNGVNGHGKACPLNEPILLADGTWTTHGNVKIGDQVASVDGNPS
                     TVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEKRRVIDTFGLKRLSE
                     TKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKFSNVEPYMIERMKAE
                     LPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFIPRVFFSANKSTRIG
                     MLCGLLETDGYVEKDGTLVFSSASEELRNGVVQLVNSLGGSCRTRVKTGVTYTYKDDK
                     RHGMDSYEARIRLTREIREAIRSPRLNGRLTAHRFEGCGVFVRNVEKIGNAECLCIMV
                     DHPRHLYVTRGYVATHNTEVVGHMALEAMRQGVKTCIASLELKPGILLKRLTRQATCC
                     KMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFDYARRRYGIQLFIIDSLMKCG
                     IGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDSEEKPTGKMDVKGSGAITDLT
                     DNLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPASVLMLEKQRNGEGWEGGVPLF
                     LDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVTEY"
BASE COUNT         1921 a         2201 c         1852 g         2105 t
ORIGIN      
        1 cagagaaccg ttacgttgtt tcgtgatatt gatttctgcg atgcccctgg cctgcgtatc
       61 cgggttgtac acttcatccc tgtaaagcat cagaatgatg tctgcatccg cctctatttc
      121 tccggaattt ttcaggtctg agttcatggg acgtttattg ggtctggact ccacaccgcg
      181 ggagagctgg ctcagcgcaa tcaacggaaa accaccggat tttgccaggc ctttaagccc
      241 ctttgagatt tcacccacgg caaggtcatg acgccccgtg gttcgggttt ttatcagccc
      301 gagataatca accaccacca gcgccgtttc cggatattta atcaggtggt gtttcgttgt
      361 tgcgcatatc tcatcaatgg ccaggttcgc ctggtccacc atccagatat tgcgcccggt
      421 catccgcccc accccttgtg agaaacgcgt ccagtcttca tcttcaaagt gagccacaga
      481 tttcaggcgt gatactggca tccctccagc cgcagacacc atacgttcac caatctggat
      541 gttcgccatc tccatggtga acagaagcac accatgcccc tgctcagtca ccttgtcgat
      601 gatgtccagc gcaagttcgg ttttccccat cgaaggacgg gcggcaatga ataccaggtc
      661 tccgggctcc ataccgcctg tttttgcgtc cagttcatca ataccggtca tcaacgtcct
      721 ggatttctcc agcccctgat tccggcattc aacacgctca accacttccg gaagcacatc
      781 atcaatatgt accggctgaa tgacgccctt tcctgtcgac agtgtgacca tcatgttctg
      841 cgcatccttc agggcatctt cagctgcttc acaggtatgc gcatcacgta atttctgcag
      901 cgcctcattc agtgtttttt ctgcattgcg cagtgcggca ttgcgccgca acgctgcaac
      961 atagtgctcc agtgaagact tcacccaggt tttacgcccg gtatcagtaa tcaccggggc
     1021 aagttccggc atctcattac acaacagcac ggggtcaatc actcctgaaa cacgggcctg
     1081 tctgcagatg cctgtataga tatcctgata cgctcgtaca gaaaaaacgt ccgctggcag
     1141 tgtggccaga atatccatca cttcaggatc tgctccgcgc agaaagaacg cgccgatgac
     1201 agcgccttca aggtcatcgt tacgccatac tggagttgtc atgcagccac acctctgata
     1261 cgagaacggt aactgggcca gttaaacgac aaccagttgc gtcccccgtc tgtgatcctg
     1321 tcggcaattc ggggactgat gaacgcccac aactcttccg gtgagaggtt actgatcaga
     1381 atggtgggca agatactttc gtacctggcg ttgataattt cctgcaaaat agccatttca
     1441 gccgcgctgc caaactgaac gccaacttcg tcgatgatca gcaaatccat tgacgcataa
     1501 cgctcaataa cttcatccgc tgttttttcg ctgtcattcc gccagcaatt tttcacagca
     1561 cgggtaaggc gcatcacgtc ggtgatctcc acactggcca gatagttacg gatgatgtgt
     1621 tttgccatag ccacagccag atgatttttt ccggtaccac aactgccggt cataacaaga
     1681 ctggtaccgt tctccagcat atctggccaa ttctccgcat agcggcgaca ggccgcaaga
     1741 tttctggctg cgccaggatt aacctccaga taattatcaa actcgcagtc ccgaaaacgc
     1801 agggcaattc cggcgttatc agtcagctct tccgccttga tggacgacag ctccatggtc
     1861 aaatcgttgg cctcagcgat taagcagtca gggcagcatg aaattttttc tctgtcctcg
     1921 ccattacgat cgctccacac cagtatatgc gtgtgatatt cgccatgttt ttcgcaatat
     1981 ccgcgacctt cacgcatcag gcaggaacga taaggccatg gcttttcgcc cttctgagca
     2041 aatgcaatct ctgcccgtaa ctcatccatt cgcgcctgta gtcttttttg ttgttcacgc
     2101 aggttaaacg tcatcatcgc tgtcacctca gaatgtcagt ttgtcactgg atttaccgaa
     2161 tttgtcagac atggctccca ggccagccag gacatcgacc tgtcgctgtc gcccacctcc
     2221 gggagcggct ggctgttgcc agaagtcttc gaagtgacga tcgggtccaa agaacgtcgc
     2281 agcctgcttc acgaactgtg tgccggtatt tcctgtggca cgtacccagg cagcatagcg
     2341 tttcacgcca tcaagcatgg cttcaggttt tattccctcc ctgagccggg ctttccaggc
     2401 tttgaaggct gcggattttg agttaccacc agcacgtttt ggatattcct gccaggcctg
     2461 ttcaaattcc ggtgaatatt cctgtcggga agaacgcgct ggtgcagacg cgtcagcaga
     2521 tgcgccaata gtgtttttac tctctgtagt attctctgaa gtaatctctg ttgtattctc
     2581 tgtaagatcg aaattggttt tcccttctcc gcggcgaggg gtttcccgtg tccgcggtga
     2641 aggctttccc tcctccgcga aattgggttt tacagtttcc cgaaaacggg tttccccatt
     2701 tcgggaaact gattgttttc attgataatt tcattaaggc gctcacaatc tatacggtag
     2761 aacattttgt gctcaagacg cttgttggtt tcaaccaaaa tgcctctgga cacaagatgc
     2821 ttacgcgcta cagcctgttg ttcaaatgta agtccggttt cgtgttgtat ctcttcacgc
     2881 gttttatgta cgccttccgc tgcatgtgct ttatcctacc agtaaaaaat ctgaccaaag
     2941 aaaataacag cgtgcggact tcccatgtat ttaacgagcc cagggtaata agcaaccgga
     3001 tgtccaaaat cgagcagaag atcagacgga cgcatagcca cctcccaggc gtttaaacat
     3061 ttttccgggc tgaaacgcca ccagcggata actcagggta tgagtacgtc cctgaacctg
     3121 gcagacaacc ttctggcttt cggtactgac caggcaaacc cgcagaacgt agccgttgct
     3181 ggtggtgaac cactgcccca cacgggggca atggttgtat cggtgataca ggaaattaac
     3241 gatgtggcgg atcatgggag cacctccttg tcagaaccat tcagcctgga atcaacaagt
     3301 gcagcaccaa aaacagcatc acctacacgg tcgtacagtt tgccagccag cggagattca
     3361 acggccttaa gcattgggta aagctggctt gtccagattt gatggatttc acgcaaatgc
     3421 aggtatacgc ctctggcgtt ttgtgcgaca gatgacatgt cagccgcacc agctcctgat
     3481 aagctcctct ccatctggtt aaaggcattg atgtatgcct ctttgaaccg ggcggcacgt
     3541 ttaccagtga agcccatagc aaggaaggcg aagccgtcgc gggtaatttg gtagcaggga
     3601 agtttgcgag taccgccgtt gggctggcgt accaaaattg atgtctccgc aaaattgcgg
     3661 gcacaaaact ctggagaaca atccaaaatg cggatctttt tcagaacatc gtcatgacgt
     3721 ttagagaaga agttggcaac agccagggat gaagtaacag cctgaccatc aacgatggca
     3781 atttcaggtt gagagagggt tgggagagta gtcatggtga cagcccctat gttgaattca
     3841 aagaactcac cacatgggac gccaatcaca gaggtggtga gacgtacagg gttggcgtaa
     3901 ccggtcaaca tagaacccgg cgcatcttgc gatgcccctg cacgccccac cataatttgg
     3961 gcgtagcaat gctcatgaca cgaaaaaacc gcatgagcgc ggttatgctc tatattgaat
     4021 ttcaggacgc caatcccggc acccgcttta taaggtgcag agacagtgta acgtcccgaa
     4081 attgcagaat caatatattg tgcagatata acgattttgt tatgttttgc tgtatgaact
     4141 acactatcga atactacagt gaagaggtcc gactggaggt cgatcagctt ccattgagta
     4201 tgcgtgccag ataccagcat cttgttgaac gcatgaaggt atacggcagc aatctcggag
     4261 aacctcacac cagcgccttt ggtgacgggc ttttcgaact cagaattaaa ggcaacgatg
     4321 ggatcgcgcg tgttttttac tgcactctga cagggaaacg catcatcatg ctgcatagtt
     4381 ttgtaaagaa aacgcagaaa acaccgccag ccgaacgcaa gaaagctgaa accagaatga
     4441 aggaggttaa gcatgactgg taaacgcact ctccccacca tgacacacga cgaaatggca
     4501 gccaaatgga tggaagaccc ggcttttaaa gcagaatacg acgctatcgc tgacgaattc
     4561 gcactgcttg atgaaatgct ggcagcacgc aaagaagctg gcttaactca ggctgaagtt
     4621 gccgagcgaa tgggaacaaa agcgaccgcg atcaccagaa tggaaagtaa tctcgcatca
     4681 ggtatcagcg gcccatcatt tgccacacta aaaaaattcg cccgcgctac tggaaaaaaa
     4741 ctccagatcc gcttcgttta accacaccgc gccgtcattc tggcggcgca ccggataaat
     4801 agtcatttcc tcgcacgatg tcttagccac cggatatccc acaggtgagc cgtgtagttg
     4861 aaggttttta cgtcagattc ttttgggatt ggcttgcgtt tatttctgga gcgtttcgtt
     4921 ggaaggtatt tgcagttttc gcagatgatg tcggtgaaac ttcgtcgctg tcgcctcatt
     4981 catacctcct gtcggtaaat ctgacaccct gaccaatagc ccaggctgtt gtgtactcga
     5041 tcagacttgc catgcgcttc acgctcatct gcgcgctgct ttcgcgaatg ttgacgcatt
     5101 ctccttcaag gccgggcaaa acatcagctt cctgttttgt tgccactgca tgaccgctga
     5161 tcaacaaaac cttccattgt tctggtttta accatttgcc gcaccattga acctgacgag
     5221 cgatatccgc cagcatcgcg tgaaattttg cgttctggtc aagatttcgc ttgtagtcag
     5281 taatgcggat ggtgactggc ttgtctttat cgagaggagt tgagaggatg gcatttattg
     5341 cggcttgctg ttgttgctta gttcggagga agattgtttg cttcatcgaa aattcttctc
     5401 tttaattcca gcggctctga tagctttcat tactgcaatt accgttttgt ccttcccatc
     5461 ctcatgcccc atcgcataag caccttcttc accatctttc caaaagtcgt cattcgattc
     5521 gggccagtcg atatccagtt caatagctgc tcgcggtgcc tgccatatca cccaggcaaa
     5581 ctcttttaat tcatcgtctc ctgtgaactg gcttttgtct tttgaccacc agttttcaaa
     5641 ctgtcggtag ctatcgttca cttccctctc ccccaaataa aaaggcctgc gattaccagc
     5701 aggcctgcta ttagctcagt gatgtagatg gtcatttaat actccgtcac gttttcctgt
     5761 cgccacgcct cgtcatattc cgatttcggc atattggcga tgtagctata tggcgatcct
     5821 gattcaagtt gcaggaactg gtgcgattgc tcgtcaagga acaacgggac accaccttcc
     5881 caaccttcgc cgttacgttg tttttcaagc atcaaaacag atgccggaga tgccagtagc
     5941 tgttcgtcct tctctgacat cttttcacca ctctgaactc tctgtaacgc tctctcgcga
     6001 gccttgttac gccagatgat gaaaaggttg tctgtcaggt ctgttatcgc tccagagcct
     6061 tttacgtcca ttttcccggt tggtttttct tcgctgtctc cttttcgcga gtgagtaacg
     6121 agaatgacgt gggagtttgt tttgtttttg aagtcgcaaa tcgagtcaac aaacgccttc
     6181 tgcccgttat agtcatcgtc gcctatgcca catttcatca ggctgtcgat gatgaataac
     6241 tggatcccgt atcggcggcg agcgtagtcg aatatttcga tcagcctgtc ggctttcgcc
     6301 gttccagtca ggccaaacac ccaaagtctt tcgtcataaa atttaaatgc agagtcaatt
     6361 tccagcactg gcggcatctt gcagcacgtt gcctgacggg taagtcgctt aaggagaata
     6421 ccaggcttca gctcaagtga cgcgatgcac gtcttcacac cctgacgcat tgcctcaagt
     6481 gccatatgcc cgacaacctc ggtgttatgc gtcgccacat atcccctggt tacatacagg
     6541 tggcgagggt gatcgaccat aatacacaag cattctgcat tgccgatttt ttcaacattc
     6601 ctgacgaata ccccacagcc ctcgaatcga tgcgcagtta atctgccatt gagtcgtggt
     6661 gaacggatgg cctccctgat ttctcttgtc agtctgattc ttgcctcgta tgaatccatc
     6721 ccatgccgct tatcgtcctt gtatgtgtat gtcacgccag ttttaactcg cgtccggcat
     6781 gacccgccga gtgagttaac cagttgaaca accccattgc gcagttcttc acttgctgag
     6841 gaaaaaacaa gcgttccatc cttctcgaca tacccatccg tttcaagcag accacacagc
     6901 atgcctatac gcgttgattt atttgcggaa aaaaacacac gagggatgaa tttgtttttt
     6961 gctgtgcacc ccattagtcc ataacctcgc agggtctcca tgagtggatt tacctgacca
     7021 cgcgccgttg atatcagcca gtccttacca tctccagaga agttgtaatc aggcagttca
     7081 gccttcatac gctcgatcat gtatggctcg acgtttgaaa acttcacgct cccattgcta
     7141 agactaccat ccccgagaag ggagccgata acccatgcta atggctctga gtggtcgcca
     7201 aagtcaccag ttatttcagg aatcctaacg ccatttttgt gcctcttcgt ttcactcaac
     7261 cgcttcaacc cgaaggtatc tatcacgcgg cgtttctcgc ctttcgtgaa tccacgacta
     7321 gtgacctccc ataggtgatc gcctgcgcaa tcaacataac gaccatcttc aaatgtgact
     7381 cggtaaacat ctctaacacc ctgcgggaat atcccagtga cagttgacgg attgccgtct
     7441 actgacgcca cctgatcgcc aatttttaca ttcccgtgag tagtccatgt cccatcagct
     7501 aaaagaatag gctcattcag tgggcatgcc tttccgtgac cgttcacacc attgaccagc
     7561 gtcaactctg cctcacggaa ctggaattta tctgccagag attcccacgg tggattaaac
     7621 agatactgct gcttgccgta gaaagcgttg atagtgtcct ggtaaaactc tcgcgcactg
     7681 tagagttctt caggatcgaa gtaggatgcc gtgccgatgt actgccagat ttcatcctcg
     7741 gtaacaccgt tcatcaggca ttcgttgatg tctttgtacg gcagagtaac aagacggcaa
     7801 cgatgttcac cgagtcggct tgcgatttcc cttgcggctt cacgaccaac atcatcaacg
     7861 tccatcgaga tgaatatttc ctcaaacctg tcgaggttat gatactcaaa ctcaatccac
     7921 tgttgcttag cgcctttccc gccaccaaac ggcacggata acgccgagat gccgtattgc
     7981 gcatagctca tacaatcaat ttcgccttcg caaagcacaa ccgccctcac gccagcgtcc
     8041 agagcctgcc atccgaacag gcaaggttcg caatcacct
//
LOCUS       DADMGC010000161         8052 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.161, whole genome shotgun sequence.
ACCESSION   DADMGC010000161 DADMGC010000000
VERSION     DADMGC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.161"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(187..873)
                     /gene="kptA"
                     /locus_tag="JNZ73_003649"
     CDS             complement(187..873)
                     /gene="kptA"
                     /locus_tag="JNZ73_003649"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HAY8733775.1"
                     /translation="MWKRFCAGLSCRKFINPVTMTLVNKNRKSRDENAMAKYNEKELA
                     DTSKFLSFVLRHKPEAIGIVLDREGWADINKLILCAQKAGKRLTRALLDTVVATSDKK
                     RFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQFLYHGTASRFLDEIKKQGLIAGDRH
                     YVHLSADEATARKVGARHGSPVVLTVKAQEMAKRGIPFWQAENGVWLTSTVAVEFLEW
                     SFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="JNZ73_003650"
     CDS             1020..1703
                     /locus_tag="JNZ73_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HAY8733776.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="JNZ73_003651"
     CDS             1700..2161
                     /locus_tag="JNZ73_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733777.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="JNZ73_003652"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="JNZ73_003652"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY8733778.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHLESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTLFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIL
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="JNZ73_003653"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="JNZ73_003653"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAY8733779.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSAFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="JNZ73_003654"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="JNZ73_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HAY8733780.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /locus_tag="JNZ73_003655"
     CDS             complement(4704..4787)
                     /locus_tag="JNZ73_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide"
                     /protein_id="HAY8733781.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            5380..6210
                     /locus_tag="JNZ73_003656"
     CDS             5380..6210
                     /locus_tag="JNZ73_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY8733782.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(6351..7124)
                     /gene="uxuR"
                     /locus_tag="JNZ73_003657"
     CDS             complement(6351..7124)
                     /gene="uxuR"
                     /locus_tag="JNZ73_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HAY8733783.1"
                     /translation="MKSVTSVQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(7339..>8052)
                     /locus_tag="JNZ73_003658"
     CDS             complement(7339..>8052)
                     /locus_tag="JNZ73_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAY8733784.1"
                     /translation="CAIACEPFRQWVIEDNFVNGRPDWDKVGAQFVADVVPFEMMKLR
                     MLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQEQAPTLSMPEGTDLNAYA
                     TLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQNGGSWRHLTLGVAGWMRY
                     TQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLSGIFADDLPQNADFVGAV
                     TAAYQQLCERGARECVAAL"
BASE COUNT         2022 a         2057 c         1907 g         2066 t
ORIGIN      
        1 ggtatgaccg gaagcgacgc tgattccagc ccccaactga ccgcctgccg taaatcactt
       61 taatttcagc agttgttatt aaaggatacc cttttacgag ggtatccctt cagactgata
      121 acaaacgcta cattgccgga tgtgctgcgc ttaccaggcc tatataaccc ctgcaatata
      181 ttgaatttat tgtttttgta ccccagtcgg cataaacgac cactcaagaa actcaacagc
      241 aaccgttgat gtcagccaga ccccgttttc cgcttgccag aaagggatgc ctcgtttcgc
      301 catttcctgt gctttgacgg ttaagacaac cggcgatccg tgtcttgctc ccactttgcg
      361 tgccgttgct tcatcagcag agagatgcac gtaatgacga tcccctgcaa tcagcccctg
      421 tttttttatc tcgtcaagga aacggcttgc cgtaccgtgg tagagaaact gtggcggtgt
      481 tttttcggca aaggaaatag cgacctgcga agtcgaatgc ccctgaaccg cgcggatgca
      541 cctaccatca ctggaataac taaaacgctt tttatcgctg gttgcgacca cggtatccag
      601 caatgcacgg gttaatcgct tgcccgcttt ttgggcacaa agtatgagct tattgatatc
      661 cgcccatcct tcacggtcca gtacgatacc aatcgcctcc ggtttgtgtc ggaggacaaa
      721 acttaaaaac ttgctcgtat ccgccagctc tttttcgttg tactttgcca tcgcgttttc
      781 gtccctggat tttctgtttt tatttactaa tgtcattgtc actgggttta tgaattttct
      841 gcaactcaat cctgcgcaga accttttcca cacagcgtaa tttttcccct atgcaaaaaa
      901 cgcatagcca gaaacatact tgcattttcc ccatacccac atggctgata aggtttttca
      961 gttccaggaa ttttcctggc tcaagcaaca cacaaacaca aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct ttttttccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc tggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccctg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattct
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacttgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggg gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagcg
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtaag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaac cgctagtata ttctgaatat tccgaatagt tacgtagatg
     4981 aaaatacttg tcaccgctgc tacttaattc cggtgggaaa ttatttattt ccgcataatc
     5041 agccatgtat tatttgaaac aattagtttg taaataatat aaattgataa catatcaaat
     5101 acatcaaaaa taccattcac aggcattatt aatatagacc tcaatatagc aacatcaaat
     5161 tccaattagt aataagatat gcttttaaat tgcatgcaaa taattttaaa tgagcgtttt
     5221 ttgatacttt atagtgtttc ctacccttta atatcactgt gtagagttga tatcactaca
     5281 acagaaacat gcatgtcatt tgtttacttc tctttttact acagaaaaat ttatctattt
     5341 ttctaaacat cagttaaata ctcataaaag gtaactatta tgtctatgcc attaagcaat
     5401 gccgctaata accaattcaa tacaaataat cacttccttc atcatccaaa agtcgacagt
     5461 gagttaacac gtcgatatga atatgcacgc ctggatacgg aaaatatcta tcttctgcct
     5521 cttgctcgtg gcaataacca taattacgat ggaaaaagcg ttgtcgaaat tagaaaactt
     5581 aatattggaa aacagtcctg gccatttaat tatgtgacag atacatgcag agaattcaat
     5641 ggtattacaa caacaggacg tatgctctat cgaaacttga aaatcacctc agctttagac
     5701 gaaatatatg gtggtatatg taaaaaggcg catgctgcta cagagttagc agaaggttta
     5761 cggctaaatt tatttatgaa gagccctttc gatcctgttg aggactatac agttcatgaa
     5821 ataacattag gccctggttg caatgttcct ggttatgccg gaaccacgat aggatatatt
     5881 tcaacccttc cagcttctca ggcaaaaaga tggaccaatg agcagccaag aattgatatc
     5941 tatattgatc agataataac tgtctcaggg gtagcaaatt cttccggatt cgcccttgcc
     6001 gcattactta atgctaatat tgagatggga aatgacccaa tcattggtat agaagcctat
     6061 cctggtaccg cggagatcca ttcaaaaatg ggatataacg ttatccccgg tgatgaagat
     6121 gcgccactca aacgcatgac tctgcaaccg tcatctcttc cagaactgtt tgaattaaaa
     6181 aatggtgaat ggaattatat tggtaaatag ccactgagaa gaatgagtac taatattcag
     6241 gttatacaga tatttaaaca aaaccctgca atttacaaag taatactcat tgcgacttct
     6301 taatttaaga aagcgcaagg aacgtttacc cttgcgctta ttataataag tcaggcgtcg
     6361 actttatcca gcggccatga atcaaagaga tagccgtcaa aataaatatc gtcaacgttc
     6421 gagaattcca gcagacgttg cttaacgttt tccagatgct gccacattgc cagcttcgcc
     6481 gctcgggcat cttttttgat taacgcggcg aggatctgtt tgtgatcgcc caaccactct
     6541 ttgcgataca ggctgtcatc cagatggctg tgcaactgga tccacattgg attgttttcc
     6601 cgccactgcc aggactgacg gaataactcc accagcatgc tgttatgcgt tgcttcagca
     6661 atggcgagat ggaactgcat gtcaccgctt tcgctgctac ccggcgcgct ggaagccagt
     6721 tcacgctctt ccagttgcaa tgcctgacgc attttgacga tatcttcgcg ggtagcctgc
     6781 agtgcggcaa actcggcgat gttactttcc agtaactgac gcgcctgtaa cagctcaaaa
     6841 ggacccgcat cattgcagac gttggcatcc ggactgtctg tgttgtggct gcctgagcta
     6901 tcaagcacat agataccggc accccggcgc acttccacca gccctttgat ctccagcatg
     6961 atcagcgctt cacgtaccac cgtccgcgtg acatcaagca tttctgcaat ttcacgctcc
     7021 ggcggcagcc gttcgccagg attgtacggc gtctttatga tcagatcgcg gatcatcgcc
     7081 ccgacttcct ggtaaggtct ttgcacagag gtgacagatt tcatggtgtt gtgtccgtta
     7141 ggtgcggtta atcaaatttt tgccggatgc ggcgtgaacg ccttatccgg cctacaacgg
     7201 gcaattttac ggcccccgtt aatctcaaac tataccatta gcactggaag ttggcacgta
     7261 caggtgggca aaaaaacgaa aactggtcaa ccaaatatca gtttgattga ccagtaagtc
     7321 tgtagggtaa tcagttagtt acagcgcagc cacacactcg cgcgcaccgc gttcgcacag
     7381 ctgctgatat gccgccgtca ccgcgccaac aaagtcggca ttctgcggca gatcatcggc
     7441 aaaaataccg ctcaggccca gcagcgcttt cacgcggtct gcgccctgat actgcgcgtt
     7501 aatcttctgg aactcggcca gcatcgggtc gaccacgtca atggcattac cctgctcatc
     7561 cacgccctgg gtgtaacgca tccagccagc cacgcccagc gtcaggtgac gccagctgcc
     7621 gccgttttgc aggtgcagac gcaccgggtc cagtagacgc tgcggtaact tctggctgcc
     7681 gtccatcgca atctgccagg tacggtgacg cagagacggg ttgctgaaac gctcgatcag
     7741 cagcgtcgca taggcgttca ggtccgtacc ttccggcatc gacagcgttg gcgcttgttc
     7801 ctgcatcatc agggcaaagg ccgctttgcg ataatccggg ttagtcatgg tgtcggcaat
     7861 ggtttcatag ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc cgttcagcat
     7921 acgcagcttc atcatttcga acggcacaac gtctgcaacg aactgtgcgc ccactttatc
     7981 ccagtccggg cgaccattaa cgaagttatc ttcaatcacc cactgacgga acggttcgca
     8041 ggcaatggcg ca
//
LOCUS       DADMGC010000162         7985 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.162, whole genome shotgun sequence.
ACCESSION   DADMGC010000162 DADMGC010000000
VERSION     DADMGC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.162"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..1078)
                     /gene="mraY"
                     /locus_tag="JNZ73_003659"
     CDS             complement(<1..1078)
                     /gene="mraY"
                     /locus_tag="JNZ73_003659"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAY8733785.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKV"
     gene            complement(1072..2430)
                     /gene="murF"
                     /locus_tag="JNZ73_003660"
     CDS             complement(1072..2430)
                     /gene="murF"
                     /locus_tag="JNZ73_003660"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAY8733786.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIARTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEDCHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(2427..3914)
                     /gene="murE"
                     /locus_tag="JNZ73_003661"
     CDS             complement(2427..3914)
                     /gene="murE"
                     /locus_tag="JNZ73_003661"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAY8733787.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(3901..5667)
                     /locus_tag="JNZ73_003662"
     CDS             complement(3901..5667)
                     /locus_tag="JNZ73_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HAY8733788.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(5683..6048)
                     /gene="ftsL"
                     /locus_tag="JNZ73_003663"
     CDS             complement(5683..6048)
                     /gene="ftsL"
                     /locus_tag="JNZ73_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAY8733789.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(6045..6986)
                     /gene="rsmH"
                     /locus_tag="JNZ73_003664"
     CDS             complement(6045..6986)
                     /gene="rsmH"
                     /locus_tag="JNZ73_003664"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAY8733790.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(6988..7446)
                     /gene="mraZ"
                     /locus_tag="JNZ73_003665"
     CDS             complement(6988..7446)
                     /gene="mraZ"
                     /locus_tag="JNZ73_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAY8733791.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT         1882 a         2295 c         1983 g         1825 t
ORIGIN      
        1 gtaccttcag cgttgccaga ccaatcagga ccagcatcag cgaaataatc cagaaacgca
       61 caatgacgcg cggttccggc cagcctttca gttcatagtg gtgatgaatc ggtgccatgc
      121 ggaaaatacg ttgtccgcgc agtttaaagg agccgacctg caggatgaca gaaagcgttt
      181 ctaccacgaa cacgcccccc ataatcacca gcaggaattc ctgacgtagc agtacggcga
      241 taatgcctaa cgcaccacct aacgccagcg aaccgacatc gcccataaag acctgcgccg
      301 gataggtgtt aaaccacagg aagcccagtc ctgccccgac tatcgcggta cagacaataa
      361 ccagttcccc ggcgtgtcgc agatacggta tgtgtaaata gctggcaaag ttcatgttgc
      421 cggtcgccca cgccaccagc gcaaaaccac cggcgacaaa tacggtcggc ataattgcca
      481 gaccgtcaag accatcggtc aggtttaccg cgttgccagt acccacaatg acgaagtaag
      541 ccagcagaat gtagaacagc cccagttgcg gcatcacatc tttaaagaat gggaccacca
      601 gctgcgttgc gggcgtgtct ttgccggcaa ggtacaaagc gaaagcaaca cccagcgcaa
      661 tgaccgacat ccagaaatat ttccaacgag cgatcaaccc tttggtatct ttacgcacca
      721 ctttgcgata atcatcaaca aagccaataa caccgtaacc taccagcacc accaacacgc
      781 accagacgta cggattggac gggtaagccc acagcagcac ggagatcaca atcgctgtca
      841 ggatcataat cccgcccatg gtcggcgtac cgcgcttgct gaagtgtgat tcaggaccgt
      901 cgttacgcac tacctgacca aaggaaagtt tttgcaaatg agcaatcatg cgcgggccca
      961 tccacaatga gatgaacagc gcggtcagca ggctgacgat ggcgcgaaac gtcagatagg
     1021 aaaagacgtt aaagccggaa taatatttga ccaaatgttc ggccagccaa actaacatgt
     1081 cccattctcc tgtaaagcgc gtactacctc ttccatggca gcactacgtg aacccttaac
     1141 taaaatcgta attacctgtt gctcagcaat cagtgattta agacgcgtaa ttaacgcagt
     1201 tttatcagca aaatgttcgc caacgccgct ggcggtgctg atagcatggc tttgtttacc
     1261 catgcttaac acgcggtcaa taccagcagc ttttgccgcc tcgcccacct gtacatggca
     1321 gtcttcgctt tcagcgccca gttccgccat atcgcccacc accagcacgc ggtagcccgg
     1381 catttcagcc agtacctgaa cagctgcggt cattgaaccg acattggcgt tgtaggagtc
     1441 gtcgagcagc aactggtttt ctgccagttg gatggggaac agacggccag gaacagcttt
     1501 cagatttgcc agccccgctt tgatagcatc aagcgttgcg cccacggaca tggagagcgc
     1561 agcggctgcc agcgcattcg caatattgtg acgccccggc aacggcagca gaacatcgac
     1621 gctaccggtt ggggtttgta gggtaaattc cataccgtgc gaggtcacat ggatattggt
     1681 ggcggtgaaa tcgctgttgg cggcattggg tgagaaacgc cacactttgc gtgagccaat
     1741 tacgctctgc cagttcagcc agtcgttgtt gtcggcgttc ataatggcga taccgttttc
     1801 cggcaggccg ctaaagattt cacctttcgc tttcgcgaca cccgcaagcg agccaaaacc
     1861 ttccagatgc gccgctgcca ggttgttgac cagcgccgct tccgggcgag tcagactcac
     1921 agtcctggca atctcgccct gatggttcgc gccaagttca ataactgcgt aatcgtattc
     1981 cggcgttaag cgcaacagcg tcatcggtac accgatgtcg ttgttgagat tgcctgccgt
     2041 ataaagcgtg ttgccgcact ggcttaaaat cgccgccgtc atctctttaa cggaggtttt
     2101 gccggaggac cccgtcagag caaccacgcg cgccggaact tgctggcgaa cccatgcagc
     2161 cagttcacca aacgccagac gcgtatcctt gacgattaac tgcggcaggt cgatgtccag
     2221 cggacggcta accagtagtg cgcctgcgcc gccagctttc gcctggtcgg caaaatcgtg
     2281 ggcatcaaaa cgttcacctt tcagggcaac aaacaggcag cccggcgtca gttttcgggt
     2341 atcagtggtt acagcatcaa gggtgatatc tgcaccttgc agttcaccgt tgagaatatc
     2401 ggtaagttgg ctaagggtta cgctaatcat gcaatccccc ccagcagacg cgccaccgtg
     2461 acgcgatcgg agtagtccag acgctgattg ccaacaatct ggtaatcctc atggcctttg
     2521 cccgcgacca gtaccacatc attctcttta gcctgcataa cggcgcaagt caccgcttca
     2581 gcacggcctt ccatcacttt ggcatgtccg gcatctaaca ttcccgccag aatatcgttg
     2641 atgatggcac gcggttcttc ggtacgcggg ttatcgtccg tcaccaccgc cacgtcagca
     2701 aactcttcgg caattgcgcc catcagtgga cgcttacctt tatcgcgatc gccaccacag
     2761 ccaaagacgc accacagctt gcccgcacag tgcaggcgcg ccgcctgtaa ggctttttcc
     2821 agtgcatccg gcgtatgcgc gtaatccacc accaccgtcg gtttgcctgg cgcagtgaac
     2881 acttccatac gtccgcaaac cggttgcaga cgcgcggcgg ttttcagcag atcagccagt
     2941 ggatagccca gcgccagcag tgttgccagc gcgagcaaca ggttgctgac gttaaaagcg
     3001 cccatcagat ggctttcaat ttcgccatcg ccccagcttg aactaaagcg aatggtcgcg
     3061 ccgctgtcgt gatagttcac ttcggtcgct ttcaaccagc gtccgtgaca gttcggattg
     3121 atatgatctt ccattgatac cgcaaccgcg tccggcagtt ttgccagcca gcggcggccc
     3181 acttcatcgt cggcgttaat aatcgcctga ccgcaatgat gttcagaata aagcagccat
     3241 ttcgcagctt cgtagtgttc catatcacca tgataatcaa ggtgatcgcg gcttaagttg
     3301 gtaaagaccg acgccggaaa tttcaatgcc gccacacggt gctgtaccag cccgtgggag
     3361 gaaacttcca ttgcgcaaaa agtcgcgccc tgatccacca gccccgccag ctcatgctga
     3421 acatcaactg ccgaaccggt agtattttct gtcgggatca ctttccccag cagaccgtta
     3481 ccaacggtgc ccattaccgc gctggtttcg ccaagcagtt ggctccactg cgccagaagc
     3541 tgggtcgtcg tggttttgcc gttggtgccc gttacgccta cgagacgtaa attgtcagag
     3601 ggttcatggt aaaagcggcc cgccagtgca gataaacgct cgttgagctg gctgagatag
     3661 atgaccggta cgccgtgcat ttcacggatt tcaccgtcgg tcgcctcatc tttcgcctct
     3721 gcaataatgg cagccacacc ttgcgctatc gcctgcggga tatatcgacg cccgtccgcc
     3781 tgatgaccta ctacagctac aaagagatcg cccgccgcag ccacacggct gtcgagtgtc
     3841 atctctcgca gtgctcgcga aggtgcgtct ggcacccacg gagcaagaag gtcgcgcaaa
     3901 ttacgatctg ccacctgtcc cctcgccttg attaatcaca aattcatttt tatcgcccgt
     3961 tgtcagcgca tccggctcga tgttcatggt acgcaatacg ccgcccatga tggcaccaaa
     4021 gaccggcgcg gaaacggcgc cgccgtagta tttacccgcc tgcggatcgt tgataacaac
     4081 aaccagcgcg aagcgcggct gactcgcagg cgcaacgcct gcggtataag caatatattt
     4141 attgatgtag cgaccgtccg gcccgacctt tttcgcggta ccggttttaa tggcgatacg
     4201 atagccttta atcgccgcct tcacgccgcc gccgcctggt agcgccacgc tttccatcat
     4261 atgcaccacg gtgcgaacaa tggattccgg gaagacacgt tcaccgggaa ccggggggtc
     4321 aactttggta atcgacagtg ggcgataaat gccgtagctg ccgatagttg cgtagactcg
     4381 cgctaactgt aacggtgtta ccattagccc gtagccgaaa gagaaggtgg ccctctctat
     4441 gtcagaccac cgttgttttt gaggatataa gccactgcgt tctccgacca accccaaatt
     4501 ggtcgctttt cccagtccaa aacgtgagta agtatctact aacgctgagg acggcatcgc
     4561 taacgccagc ttggaaacac cgacgttact cgacttctgt aataccccgg tcagggttaa
     4621 ttcgctgtag cgtgccacgt ctttgatttc gtggccgtta attcgataag gaatggtatt
     4681 gagtaccgag ttttcccgca ccacgccacg ttgcaacgcg gtcatcacca ccatcggttt
     4741 aaccgttgag cccggttcaa acacgtcggt gatggtacgg ttacgcatcg cctctttcgg
     4801 cgtgccgctc agattgttag ggttgtatga cgggctgtta gccatcgcca gcacttcacc
     4861 tgtgttgaca tccaccagca cggcgctacc agattcagcc ttgttaaagg ccaccgcatt
     4921 gttcagttcg cgataaacca gcgcctgcag gcgttcatca atactcagcg ccaggttgtg
     4981 cgctgcctgg ctgtcagtag aagaaatatc ttcaattacg cgaccatagc ggtctttacg
     5041 cacaatgcgc tcacccggct gcccggtaag ccatttatcg aaactcttct caacgccctc
     5101 aatcccttga ctatcgacgt tagtaaagcc gatgaggtga gcagtcactt cgccggacgg
     5161 atagtaacgg cgagactctt cacgcagatg aatccccggc agtttcagtt ttttgatgta
     5221 gtccgccatg tcagagttta cctgacgcgc cagataaata aagcgccctt tcgggttggc
     5281 gttaatgcgg gcagaaagct gatccagcgg aatattgagc gcgttagcca gcgccttcca
     5341 gcggtcaccg acgctgatac cgccagcgtc atgcacttct ttcgggtcag cccaaatcgc
     5401 ttttaccggc acgctcaccg ctaacgggcg accagaacgg tcagtaatca tgccgcggga
     5461 ggtggaaact tgctgaacgc gaagagaacg catgtcgccc tctttcacca gcatatccgg
     5521 ggagataact tgtaaccacg ctacgcgtcc gagcagaaaa gccagcgcca ggagaataca
     5581 gccgcataac aacgcaaaac gccaactgat aaagttggca tgttcttcct gacgttttgg
     5641 tttctgcgtt ttcgccgctg ctttcatgcg tcgcgtttat ccttattttt gcactacgat
     5701 attttcttgt gacggatcaa catgctgcat ttgcagcttt tccgtggcga tcctttccac
     5761 ccggctatgg tcgccgagcg cattctcttc aaggatcagg ttgcgccatt caatgtctaa
     5821 agcatctcgc tccagcacca gttgttcgcg ctgagcggtc agtaaacggg tatggtgcgc
     5881 cgtggttacc acggtcaccg ccgtcaaaat aatgcaaatg aacaggcaga gtggcagctt
     5941 cccaaatcgc aaaagatcgt caccgataac accaggcaat gcatggcgct cgtggcttcc
     6001 catcgatcct ttaactttgc ttagagcttc tgtcactctg ctgatcatgc gttcgtcctc
     6061 tctgcaatac gcagaactga actacgggca cgagggttct cagccacctc ttcttcgccc
     6121 ggcattaact tgcctagtgc tcgcagctga cggccaccca gttttttgag ctgctcttca
     6181 gtcatcggta accctgccgg aacttgcgga ccgcggctgt tttcacgcat aaaacgtttc
     6241 acaatacggt cttccagcga gtggaagctg atgatcgaaa gccgcccacc cggggccagc
     6301 acgttgagcg agctttttag cgcctgctct atctcctcca gttcactgtt tacccaaatg
     6361 cgcacagcct ggaaggtacg ggtcgcggga tgtttaaact tatctttcac cggcgttgca
     6421 gcagccacga cttccgccag ttctttggtg cgggtcatcg gctgttcgcg gttacgctcg
     6481 acaatggcgc gggcaatgcg tttggcaaaa cgctcttcac cataggtttt caatacccag
     6541 gcgatatcgg cttcttctgc ggtttgtagc cattcagcgg ctgactgccc acgggttggg
     6601 tccatacgca tgtccagcgg accatcgcgc ataaaggaaa agccgcgttc agcatcatca
     6661 agttgcggtg aagagacgcc aagatcgagg agaatgccgt cgatcttgcc aataagatcg
     6721 cgctcggcaa cgtattcgcc cagcgcggag aaaggtccgt ggatgatgga gaagcgcgga
     6781 tcatcaatag tcttcgcaac ggcgatagcc tgcgggtcgc gatcgatcgc cagcaaacgc
     6841 ccctcttcgc caagctgcga gaggatcaga cgtgagtgac caccgcgacc aaaagtccca
     6901 tcaatgtaga tgccatcagg acggatattg aggccattaa cggcttcatc cagcagcacc
     6961 gtagtatgtt tatagttttc catcatttta tagagacaag tcctgcagtc gctccgataa
     7021 gtctccggta gccaactgct ctgcgtcgat atcttccttg acctgttgat gccaggttgt
     7081 ttcatcccac agctcaaact tgttgaactg tccaaccagc atcacttctt ttgtcagccc
     7141 ggcatgttgc cgcagtactg gcgcgattaa caatcgacct gcgccatcca tctgacattc
     7201 gctggcatga cctaacagta ggcgctgcac acggcgctca accgggttca tgctcgacag
     7261 acgcgataat ttttgctcga taatttccca ttcaggcagg gggtaaagca gcaggcacgg
     7321 gtgatgaatg tcaatggtgc aaaccatttg accggcagcg ttctcaagca gctgttcccg
     7381 ataacgggta ggcactgata agcgcccttt gctgtcgaga ttgactaacg ttgctccccg
     7441 gaacatgcca gcctcacccc ttattcccac tttgccccac aatttcccac tgaaaggagt
     7501 ttacggagcg gaggaaaagc ttgtcaagcc agtcacaagg cataccgagg taaaaaaccc
     7561 gatgtttacg gctaatatca gcgttgttta ttttacgtcc aacgtgcgaa gcaaattaac
     7621 gttatgaata gctgtaagaa aaaatccctc atcgtcatca ctgttttaaa atgactcact
     7681 attttccaca gcaaatataa agtgtcagtt tgcgacgcga gcggcatttt aggacatatc
     7741 ttccccggtt aacagtcctt gttacgtctg tgtggcgctt gatgccagcg ggtttgcgca
     7801 aaagcaaaga aaagtgtttg ttaaatcggc aattcatcgc cgcatgtaac aaaataatgc
     7861 aaaaacgcgc tgtcgatatc acttaattat tgtgaatttc agctggtgag ttaattaacg
     7921 ggcgggttta aaaccattta tcaataatag tcaggaatag tcttatttac tttaaggata
     7981 ttgat
//
LOCUS       DADMGC010000163         7860 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.163, whole genome shotgun sequence.
ACCESSION   DADMGC010000163 DADMGC010000000
VERSION     DADMGC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.163"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            82..409
                     /locus_tag="JNZ73_003666"
                     /pseudo
     CDS             82..409
                     /locus_tag="JNZ73_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(439..3351)
                     /locus_tag="JNZ73_003667"
     CDS             complement(439..3351)
                     /locus_tag="JNZ73_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733792.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGSIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3354..3470
                     /locus_tag="JNZ73_003668"
     CDS             3354..3470
                     /locus_tag="JNZ73_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY8733793.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3834..4676
                     /locus_tag="JNZ73_003669"
     CDS             3834..4676
                     /locus_tag="JNZ73_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY8733794.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4679..5767
                     /gene="capU"
                     /locus_tag="JNZ73_003670"
     CDS             4679..5767
                     /gene="capU"
                     /locus_tag="JNZ73_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY8733795.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNEPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5772..6722
                     /gene="virK"
                     /locus_tag="JNZ73_003671"
     CDS             5772..6722
                     /gene="virK"
                     /locus_tag="JNZ73_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY8733796.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNQIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6787..7731
                     /gene="lpxM"
                     /locus_tag="JNZ73_003672"
                     /gene_synonym="msbB"
     CDS             6787..7731
                     /gene="lpxM"
                     /locus_tag="JNZ73_003672"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY8733797.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2116 a         1763 c         1861 g         2120 t
ORIGIN      
        1 ctcctgctcc gacatacctg caggcaaagg ccatgaaagc cctgatttcc ggaagcgagc
       61 gaacagtcca caaactgttg aacgtggtgt cagcggcgga cagggataag ccctcgtcag
      121 cagttccgtc agcactgcga cagcgttctt tacccggtca aaacagcgtt gcggtgcacc
      181 acgcctgacg gatgaactgc gtgctcaggg ttaccacttt aacgtaaaaa ccgtggcggc
      241 aagcctgcgc cgtcaggggc tgagggcaaa ggcctcccgg aagttcagcc cggtcagtta
      301 ccgcgcacat ggcctgcccg tgtcagaaaa tctgctggag caggattttt acgccagtgg
      361 tccgaaccag aagtgggcgg gagacatcac gtacttacgt acagatgaag ttcggttaca
      421 tcctgtcagc acggaacctc acgctttttg agcggcacga cggatccccc ccacccagta
      481 tccgacagac ttttcactgc gttcgtaaaa acccttcttc cctcccagca tatccactaa
      541 ttccggtgac atatcctgca gataagtctg caaatcatgc cccagatctt ccggtgactg
      601 caactcataa accgcgcgcc acacatcacc ctgccttatt ttaagccccg ggttaagaat
      661 atactgtccg tggttcagcc tcagaaaaag cttttttcca taaggatttt tgctgttaac
      721 ttcatgttga gagagctggc tgctgatata actccttcgt ttgcggcgtt ccggcagcaa
      781 tgcttcagga agatgaacca cacactctgc cagtcgtgcc gcactgaagg cttccgaaaa
      841 aaacagagca cttcccagat gcgtgtggaa cagcgcctgc atcaggatca ccagaaacat
      901 tgccatctgc cggttgtcca gtttgatgag gcgtccttcc acctgcagtg acagactttc
      961 aggtttcagc agggtataca actgcgcact tttctgttgc gcataacggg gcgtactgac
     1021 cgccgctgac agaacttgat gataagccgc cagcccgtca ttaccggtca gcaagggatc
     1081 tgcaccacgc tccagcagta actgaaccag tgcaatatta cctgcccggg ctgctgacat
     1141 cagcggagta agattaaacg gcgaacggtg atcaacacca taacgttcaa tatcccgcaa
     1201 cacggcaacc gggttattca ttgagtatgg ccggaaatac ttcagctcca gtaactgcag
     1261 agtcttcgtt ctgggctgcc ttgtgggctt aaaatcttcc cggtacagtg cattatacag
     1321 cgggaacagg gaataaatca gactgtactc cagcgcctgc agttgtatgg ttttatcggc
     1381 tgtacctgcg aggatatgct gtttccactg tcgtgcatcc tctgcggtga taatctgcca
     1441 tggcatctca gatgtctgca ggatccggct acggatggtg tccgcctgct cctgcttccc
     1501 ctgtttttca agcagatgtg cggttttctg ccactcatca cggctggaaa tctcttcctg
     1561 cagattcaga ggctcttcct gatgcgtgag cgcaagcaga ctccacagag gatgctccac
     1621 ctgctgttca acaagataca cattgtgtgt agcacgggtg agcgcaacat acagcgcgtt
     1681 ggtaaaaaat ttatagattt ccgctgaacg atcctgcctg tcccgcggac gggaataacg
     1741 ggcctcgccc tcaagatcag ccggtgtaag accctcgcaa atatcatcaa atgcctgccg
     1801 ggcagcgctt actatattgt aaaggatcac cgtttcgtat tccagcccct taacctcctg
     1861 aaccgaaaag accagcggcg tactgaacca gcatcgcgcc tgtgctttct gttccggatg
     1921 catcacaatg accgctacac gattcgaaag tgaggttttg gcattcagtt cctgctttgt
     1981 ctcttccctg tcgtccagca accggatgct cccttctgcc tgaccgcagc ttctgacaaa
     2041 atggtgactt tcccgatcaa tagcactaaa gcggacctgt ttgagtctca gcaaccggtt
     2101 agccagtgca gtgacgtggt gcccgttacg gtaattcgcc tgcaggatat taacggttgt
     2161 gtcgtttccc tgttgctgcc ggaaaaagag acttttgagc gaactccatg agaaaaagct
     2221 cggatgaact atctgattgg catcaccaca gagtaaaaac tgaccgggat gacgtagcgt
     2281 tttcagtacc agttgcagtt gaacaggcgt catatcctga acttcatcaa caaaaatgac
     2341 atcataacgg ggagttgcca acgaaagata ctgatgactg agcagattac tgtcatactg
     2401 atgcgactgt ttcagccatg cgatataacg ttcaaacagg acatagaccg tcgggcggtc
     2461 ctccatgcca tagagcgact gccgtatccc cagtgacaga tacgcctccc gggacagcgg
     2521 cccattcccg gaggcaaccg caccgatcac gcccctgaac tcctcataca gggtatgggc
     2581 agcccccagt gcggcgatag ccctgttgcg gggcagccag tcgctgaacg cactgaaagt
     2641 cacctccctg ccttccggaa tgcgcagagt ttccagaaat tccgtcagcg aaagaaagtc
     2701 aatattctgt acctcactcc cttcccctga agcgtcatag agagtacgcg ctttttccac
     2761 cagaaatgag gaaaggctga gatacagtat atcaccagca gcctgcttca ttttttccag
     2821 taccagcgcc gtcttaccac ttcccgccga gccaacaatc accagcggta gcgggtgttc
     2881 gtacagtgcc tgctgatcat catcaaagga cagcatttta tccaggcggt ggaaacgccc
     2941 atgagacgga ttgatatacg tcagcgcttc agtcgcgata tcaacaggat ccggcactgg
     3001 ttgttgttgt aagcgccctt catcaatcac cacgtttctg cgaagaaaac gtgaagtgtt
     3061 ataggcatga ttgcgaatat attccagaac aaggcagata gtttcaccgt ggtagcgata
     3121 cagcgaaaac agtaatcggt cccggatact gagccgggcg cggtaaagat tggtgtcaat
     3181 tttacgaaca tcagcctgaa caaagttatc ggtggcaata gcctgtgcaa attttttata
     3241 acccgggatc gtgtcaggat tcagagtatg ataaagacag attttcatgg ctgccggttt
     3301 ccgtcgtgca aaaaacatgc tgattttacg aaggaatgcc ttactgaaca accttgcccg
     3361 gaataccccc cgaaatcaga tggaacaatc agaaaaatgc tctgcagtta tggaataaag
     3421 aaggtggggt ggtacgccgg gagggcgatc cgctggaaat ggcgcagtag ctgcaggcct
     3481 gtcatgatgc cggaatagaa gttcgtgtac aggttaatga aagcgtcacc ccgtaaataa
     3541 actctgcgct gaaattcctt tttttcattg gagcacctgt gttgttctga ggtgaacata
     3601 tcacctctgt tcaggtagcc aaattcagtg tgccacctca aggttcttag tgagtcgttt
     3661 ttaaaagata tgataatata tcattgttta tgaattgttg atgtttaatt tagtaaatta
     3721 tatcaaatat gccatgggtt tgctatgtta ttcatccagt atctggaaat aaaatgcacc
     3781 agctttattg ggttttcctt ttgtctctgc ttactaggtg ccagaaactt taaatgttaa
     3841 acgaaggggg cattttgttc aaagccaatc atgttccggt attaatgtat catcatgtca
     3901 gtcattgccc cggactggtt acactttctc ccgtgacatt ccggaagcaa ataaaatggt
     3961 tggctgaaaa caactggaaa acactgtcat cagatgaact ggagtttttt tatcggggcg
     4021 gaaaactccc ccggaaaagc gttatgttga catttgatga tggatacctt gacaactggt
     4081 ttcaggtata tcctttatta aaagagttta atctcaaagc acatattttt ttaattaccg
     4141 gttttattgg gaatggtccg gttcgtcact ctcctggaaa ggaatattct catcgcgatt
     4201 gcgaacatca aatagcgaca gggaatgctg ataatgttat gcttcgctgg tctgaagtaa
     4261 acgaaatgtt gcaatctggt ctggtcgaat ttcatgttca tacgcatact catacacgct
     4321 gggataaaaa gttcagttca cgagaagaac agtgtaagca tcttcggcag gacttgttga
     4381 gcgggagaga atatttgaaa gaaatgacag gaaaatgcag taaacatttg tgttggccgg
     4441 aaggttatta taacaaggat tatattcagg tcgctgaaga acttggattc tattatttat
     4501 atacaacaga aagaagaatg aatgctcctg ctaaaggaac tacacggata ggaagaataa
     4561 gcaccaaaga acgggaaagt tgcgcatggc tgaaaagacg acttttttat tatacaacac
     4621 cgtttttttc atcgttgctg gcctttcata aggggccaag attaccggat gattaataat
     4681 gaatatacta tttacggaat catcaccgaa tataggtggt caggaattac aggctgttgc
     4741 tcaaatgaag gccctgaaga aaatggggca ttcagttctg cttgtctgca gggaaaacag
     4801 caaaattgct tttgaagcca gtaaattggg aattgatatc acattcgcgt tatttcgaaa
     4861 cagtcttcac atccctactg catggagatt actcggaata gttcatggtt ttcagcccaa
     4921 tgcaatcgtt tgtcacagtg ggcatgatag caatattgtt ggtttagtac ggttattcac
     4981 taggaaacat ccattcagaa ttatcaggca aaagacatat ttgacacgaa aaacaaaagt
     5041 tttttcaata aatcattttt gcgatgaggt gattgttccc ggaacaagta tgaagacaca
     5101 tctggagcag gaaggatgtc gaacccgggt tactgttgtg cctccaggct ttgatttcca
     5161 gaaattatac gttgattccc gaaacagttt gcctccaaat gttctttctt ggctggcgtc
     5221 ccgaagggga tgccccgtta ttgctcaggt aggaatgttg cgcccggaaa aagggcacga
     5281 atttatgttg aatttactgt tccatttaaa aatgaatgga cgacagttct gttggttgat
     5341 tgtggggtct ggttcgcctg aactgcggga gcatttacag tatcagattg acagtatggg
     5401 catgcatgat gatgttttta ttgctgacaa tgtttttcct gccgcccccg tatatcgggt
     5461 tgccagtctg gtggttctgc cttcagaaaa cgaatcgttt ggtatggtac tggcagaagc
     5521 atcggcattt tctgtgcctg tactggccag tcagattggt ggaatccctg atgttattca
     5581 gaacaaccag accgggacat tgttaccagc aggtaataag cacgcatgga tgtgcgccct
     5641 gaatgatttt tttaatgaac ctgggcgttt ttatcagatg gctcgccagg caaaacagga
     5701 tatagaagag cggtttgata ttaataaaac tgcgttaaaa atactcacat tagcgaagca
     5761 caagtaacaa tatgttttct gtaagtaact tatcatttat cggtttcctt aaaaggattg
     5821 ttttttcctc agattcactc ccggggaagt gggaacacag aaaattccgg ttcatgtaca
     5881 ttttgcgatg tgctataaat ccggttgcca gtattcgata ttattacgaa ctgcgttcct
     5941 tgcagtgcat tgaggatatt ctggcaatac aacccacgtt gccagcaaga attcatcgac
     6001 cttatttgca taaagggggg cgagcctggt cccgggggca atatattctt gagcattatc
     6061 gctttgtgca aaatttgcca gaaaaatatt ctgaattttt gtttccacaa aaatcggtat
     6121 cattagtcca attcatcggg aaagatggag aggattttga tatccagtgt tctcccagtg
     6181 gttttgaccg ggaaggagag ttgatgttgt cattgttttt caatcaaata gtgattgccc
     6241 ggttaacatt ttcagtcatt ctgactcaaa atggacacac cgcttttatt ggcggattac
     6301 agggagcccc caaaaatacc ggacctgatg ttattcggtg cgcaacccgg gcctgttatg
     6361 ggcttttccc aaaacggatt atttttgaag cattttgtgc gttgatgaaa gcttgtaatg
     6421 tctctgagtg tctggcagtc agtgagcaca gtcatgtttt ccggcaattg agatactggt
     6481 atcagaaacg caaaaccttt gttgctgtct acagtgattt ctgggagtct gtagcaggga
     6541 aaacctgtgg tgactggtac aaattaccaa cacaggtggt tcgaaagccg ctaagcaata
     6601 tagctagtaa aaaacgctct gagtatcgaa aacgatacgc tttgctggat tatatccatg
     6661 aaactgctat ccgctctttg gatgcatatc ctgtaaactc agaacatcag gacttaaatt
     6721 aaggtttgat gtattctctg aataaaatat taatgatgat tttggtaggg gcattcgcac
     6781 taaataatga aaaaatacaa atccgagttt attcctgaat ttaagaaaaa ttatctttcc
     6841 cctgtttact ggtttacatg gttcgttttg ggaatgattg caggtatttc aatgtttccc
     6901 ccttcattca gagatcctgt cttggccaaa atagggcgtt gggtgggcag attgagcaga
     6961 aaagctcgtc gcagggcgac gattaattta tcgctttgtt tcccggaaaa gagtgataca
     7021 gaacgggaaa taattgtcga caatatgttt gccacagcat tgcaatccat agtgatgatg
     7081 gcagaactgg caattcgtgg tccggaaaag ttccagaaac gagtgttctg gaaaggcctc
     7141 gaaattcttg aggaaatccg gcacaataac agaaatgtga tttttctggt tccccatggc
     7201 tggagcgtgg atattcctgc aatgttgctg gcagctcagg gggaaaaaat ggccgccatg
     7261 tttcatcagc aacgaaatcc agtgattgat tatgtctgga attctgtacg gcgtaaattc
     7321 ggggggcgct tacattcccg ggaggatggg ataaaaccat ttattcagtc agtacgccag
     7381 ggatactggg ggtattacct tccagatcag gatcatggtc ctgaatacag tgaatttgct
     7441 gatttttttg cgacctataa agcgacatta ccaattattg gacgtctgat gaacatcagt
     7501 caggctatga ttataccgct tttcccggtt tatgatgaaa aaaaacattt cctgactatt
     7561 gaagttcggc caccaatgga tgcatgcatt gccagcgcgg acaataaaat gattgcccga
     7621 caaatgaaca aaacagtgga aattttggtg gggtcacatc cggaacagta tatctgggtt
     7681 ttaaaattgt taaaaacgcg caaatcaaac gaagcggacc cgtacccttg aaattataaa
     7741 gtacaggtat ttccactagt tgtttcttac aggttaccaa tcgaaacaca tcccccttcc
     7801 gtgtgttgcc cgtttggcac ggaggactgt gtagatgcag ttcgttaaat ttaatttatg
//
LOCUS       DADMGC010000164         7858 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.164, whole genome shotgun sequence.
ACCESSION   DADMGC010000164 DADMGC010000000
VERSION     DADMGC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.164"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..165
                     /locus_tag="JNZ73_003673"
     CDS             <1..165
                     /locus_tag="JNZ73_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733798.1"
                     /translation="THYGGAAGPVIFGVGYNDIKSWWLVGAVLTILTFLVHITLGVWW
                     WNMLIGWNML"
     gene            348..2528
                     /locus_tag="JNZ73_003674"
     CDS             348..2528
                     /locus_tag="JNZ73_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HAY8733799.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNHQM"
     gene            complement(2669..3952)
                     /locus_tag="JNZ73_003675"
     CDS             complement(2669..3952)
                     /locus_tag="JNZ73_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HAY8733800.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(4087..5157)
                     /locus_tag="JNZ73_003676"
     CDS             complement(4087..5157)
                     /locus_tag="JNZ73_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8733801.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(5135..5353)
                     /locus_tag="JNZ73_003677"
     CDS             complement(5135..5353)
                     /locus_tag="JNZ73_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY8733802.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(5438..6061)
                     /locus_tag="JNZ73_003678"
     CDS             complement(5438..6061)
                     /locus_tag="JNZ73_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HAY8733803.1"
                     /translation="MTSQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            complement(6383..7090)
                     /locus_tag="JNZ73_003679"
     CDS             complement(6383..7090)
                     /locus_tag="JNZ73_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY8733804.1"
                     /translation="MAQLYGTDVVRIRQNHHENKSRFVEGVHFYKVVGDELRALKHKV
                     ALNYFVKSAPNARHLILWTERGAARHAKMLETDQAWDVFEKLEDGYFNQREKVANHPQ
                     PVPQPESSLDRARAMEIALNVADRLVNMFPNLSSESKQAAVAGVFNPIVGRDVVPLPA
                     ISEHYYSATEVGQRYGISANKVGRIANTYMLKTEKYGKWFIDKSAHNDKQVETFRYNE
                     AGVHKIEELIEGERKAA"
     gene            complement(7038..7472)
                     /locus_tag="JNZ73_003680"
     CDS             complement(7038..7472)
                     /locus_tag="JNZ73_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY8733805.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIGAVVRNGCCAHPAKPS"
     gene            complement(7545..>7858)
                     /locus_tag="JNZ73_003681"
     CDS             complement(7545..>7858)
                     /locus_tag="JNZ73_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001128261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY8733806.1"
                     /translation="QAYANAEFIAAFNPNVALALLDERERNLQYIKSRDQENEEIALT
                     VGKLRVELEAAENNLIDSECHVAELEEALRDKQALLEASEKRIAELEAQTVTVKEVGD
                     A"
BASE COUNT         1811 a         1956 c         1929 g         2162 t
ORIGIN      
        1 actcactatg gcggcgcggc aggtccggtc atctttggcg tgggttacaa cgatattaaa
       61 tcctggtggt tggtcggtgc ggtactgacg atattaacct tcttggtgca tatcaccctc
      121 ggcgtgtggt ggtggaatat gctgatcggc tggaacatgc tgtaaatata ctcgtcatac
      181 ttcaagttgc atgtgctgcg tctgcgttcg ctcaccccag tcacttactt atgtaagctc
      241 ctggggattc actcacttgt cgccttcctg caactcgaat tatttagagt atatccattt
      301 attatctttc tgcgcacttc acggtgcgca gatatctgga gcatttgatg atcaagttat
      361 ctgaaaaagg cgtgtttctc gccagtaata acgaaataat tgccgaagaa catttcaccg
      421 gcgaaattaa aaaagaagaa gcaaaaaaag gcactattgc ctggtctatt ctctcttctc
      481 ataatacgtc cggaaatatg gataaactta aaattaagtt tgattcatta gcctctcacg
      541 atattacctt tgttggtatt gtacagaccg ctaaagcgtc cggaatggaa cgtttcccgc
      601 tgccgtatgt gctgaccaac tgccataact cactctgcgc cgtcggcggc accattaacg
      661 gtgatgacca tgtttttggt ttatcggcag ctcagcgtta tggcggtatt tttgtgcctc
      721 cgcatattgc ggtcatccat caatatatgc gtgagatgat ggctggcggg ggcaaaatga
      781 tcctcgggtc agacagccac acccgttacg gtgcattagg gacaatggca gtcggtgagg
      841 gtggcggtga gttggtaaaa cagctgctta atgacacctg ggatatcgac tatccgggcg
      901 tggttgcggt gcatctgacc ggaaaacctg cgccgtatgt ggggccacag gatgtggcgc
      961 tggctatcat tggcgcggtg ttcaaaaacg gttacgtcaa aaacaaagtc atggagttcg
     1021 ttggaccggg cgttagcgcg ctctctaccg atttccgtaa cagcgttgac gtgatgacca
     1081 cggaaaccac ctgtttaagc tccgtctggc aaactgatga agaagtgcat aactggctgg
     1141 cgctgcacgg tcgcggccag gattactgcc agcttaaccc tcaaccgatg gcgtactacg
     1201 atggctgcat cagcgttgat ttaagcgcca tcaaaccaat gattgcgctg ccgttccacc
     1261 cgagcaacgt gtatgaaatc gacacactga accagaacct gaccgacatt ctgcgtgaga
     1321 ttgaaattga gtccgaacgc gtggcgcacg gtaaagccaa actctcgctg ctggataaag
     1381 tggaaaatgg tcgcctgaaa gtgcagcagg ggattatcgc gggctgttct ggcggtaact
     1441 acgaaaacgt catcgcggcg gcgaatgcac tgcgcggtca atcctgtggc aatgacacct
     1501 tctcgctggc ggtttacccg tcatcacagc cggtgtttat ggatctagcc aaaaaaggtg
     1561 tggtagcaga tttgattggc gcaggcgcaa tcatcagaac cgcattctgt ggcccatgct
     1621 ttggcgcggg cgatacgcca atcaacaacg gtttgagtat tcgccacacc acgcgtaact
     1681 tcccgaaccg cgaaggctct aagccagcca atgggcagat gtcagcggtg gcgctgatgg
     1741 acgctcgttc tatcgctgcg actgcggcaa acggtggcta tttaacctct gccagcgaac
     1801 ttgattgctg ggacaacgtg ccggagtacg ccttcgatgt aacgccgtat aaaaaccgtg
     1861 tttatcaggg ctttgtgaaa ggggcaactc agcaaccgct gatttacgga ccgaacatta
     1921 aagactggcc ggaattgggt gcgctgactg acaatatcgt cctgaaagtg tgctcgaaga
     1981 tcctcgacga agtcaccacc accgacgaac tgattccttc cggtgaaacc tcttcttatc
     2041 gttcaaatcc gattggtctg gcggggaatg tcagcgagct gacagaggtg tttgcgcgca
     2101 ttaagcagat tgctggtcag gagcatattg atccgctgca aactgaaatt ggcagcatgg
     2161 tctatgcggt gaaaccaggc gatggttctg cgcgtgaaca ggcggcgagc tgccagcgtg
     2221 tgattggcgg tctggcgaat attgccgagg agtacgcgac taaacgctac cgttctaacg
     2281 tcatcaactg ggggatgtta ccgctgcaga tggcggaagt gccaaccttt gaagtggggg
     2341 attacattta catccctggc attaaagcgg cgctggataa tccgggtacg acgtttaaag
     2401 gttatgtgat ccatgaagat gcgccggtaa cggaaattac gctttacatg gaaagcctga
     2461 cggctgaaga gcgcgagatt atcaaggcgg gtagtttgat taacttcaat aaaaaccatc
     2521 agatgtaaaa agcgccatgt gaatgtaggt cgcattcggc acttattgtc ggatgcgatg
     2581 ctggcgcatc ttatccgacc tacaaatcgc atcgaaccgt aggccggata aggcgtttac
     2641 gccgcatccg gcaaatagtt aatagctctt acttcttcgc ctctgcaacc actttgctgc
     2701 ccacgccgcg gttattgtat tcccacatgc ggttgtagtt agtgtcattc agattgcgct
     2761 gtatttcgtc gttatcatct acgctgccgg tattacccgc aaacggacga ttagagatca
     2821 ccgcatcggc ccacggttta gccgtgttaa aaccttcgtt gatggcgcta tcacggatca
     2881 ccacctgacc gttggtatta gcatcaacat ccagcgagcg gcccagttgc gccacgccat
     2941 caccggaagc attgaaacgg ctgtttacgg cgaggaaacc gtaatagatg ttagacagcg
     3001 tagccggtgc aaacacatat gcttcttgct gggtacgtga gttcaccacg cggaattcgg
     3061 tgttatcgaa caccactgcg ccgcgaccag aaacgatatc cacatcccct tcaatatagc
     3121 tgttggtcac cagcgtacgc ggctgacggt tggtttccag acggttctgc acaccgctgt
     3181 tggtgacaaa gaaggtgttc tgacgaccga gaatgttaac gttgttaatc tgtacctggt
     3241 caccgtcagt acgcagtgct accgccggat ggttacctgc atctacacta tcgcccagcg
     3301 tgttttcgat ggtcagattt tgcagttgca gtccattgtt ttgtgaccag aagaccgcag
     3361 aacacagaac accgatactg tcgctgcgtt tgctctggca gctatcgtac atataccacg
     3421 ctggtttacc tggcatatat ttgccgcgcg ggttgacgtc gtgacgccag tcggcagggc
     3481 tcatgccccc atcaagggaa agcccaatct tcacatcaat cggtttttca cctgtaccgt
     3541 acagagtgat tccacctgga gcagcaggga catacaccgt tccctgatac tcaccaggca
     3601 tcacggcaat atactggcgc ttgttggtgc gcttgataat tgccgcatct accgccgcct
     3661 gaatcgtggt atgcgttaca ccttgagtac ccgccgggcc gacaacaaag tcaggttgcg
     3721 caggcagggt tatcggggac ggattccacg ctgcagcacc tggtgtcagg gatgcaaaat
     3781 agtgttgcgc atcgaaattc agcgcttctt ttgccgacag aatcgggcga gaagaggtac
     3841 caggcgcagt ttgatcagaa ggacgttgat cgggtggtgt tgagctacag gcggtcagcg
     3901 tcacgccaaa agccaatgcc agcgccagac gggaaactga aaatgtgttc acaggttgct
     3961 ccgggctatg aaatagaaaa atgaatccgt tgaagcctgc ttttttatac taagttggca
     4021 ttataaaaaa gcattgctta tcaatttgtt gcaacgaaca ggtcattatc agtcaaaata
     4081 aaatcattat ttgatttcaa ttttgtccca ctccctgcct ctgtcatcac gatactgtga
     4141 tgccatggtg tccgacttgt gcccgagaag atgttgagca aacttatcgc ttatctgctt
     4201 ctcatagagt cttgcagaca aactgcgcaa ctcgtgaaag gtaggtggat ccccttcgaa
     4261 ggaaagacct gatgcttttc gtgcgcgcat aaaatacctt gatactgtgc cggatgaaag
     4321 cggttcacga cgagtagatg caattatggt ttctccgcca agaatctctt tgcatttatc
     4381 aagtgtttcc ttcattgata tcccgagagc atcaacatgc aatgttgtag ggatggcaat
     4441 ttttacgcct gttttgcttt gctcgacata aagatatcca tctacgatat cagaccactt
     4501 catttcgcat aaatcaccaa ctcgttgccc ggtaacaaca gccagttcca ttgcaagtct
     4561 gagccaacat ggtgatgatt ctgctgcttg ataaattttc aggtattcgt cagccgtaag
     4621 tcttgatctc cttacctctg attttgctgc gcgagtggca gcgaccgggt ttgttgttat
     4681 atggccttca gctattgcct ctcggaatgc atcgctcagt gttgatctga ttaacttggc
     4741 tgatgccgcc ttgccctcgt ctatgtatcc attgagcatt gccgcaattt cttttgtggt
     4801 gatgtcttca agtggagcat caggcagccc cctccttatt gctttaattt tgctcatgta
     4861 atttatgagt gtcttctgct tgattcctct gctggcgagg attttttcgt agcgatcaag
     4921 ccatgaatgt aacgtaacag aattatcact gttgattctc gctgtcagag gcttgtgttt
     4981 gtgtcctgaa aataactcaa tgttggcctg tatagcttca gtgattgcga ttcgcctgtc
     5041 tcggcctaat ccaaactctt tacccgtcct tgggtccctg tagcagtaat atccattgtt
     5101 tcttatataa aggttagggg gtaaatcccg gcgctcatga cttcgccttc ttcccatttc
     5161 tgatcctctt caaaaggcta cctgttactg gtcgatttaa gtcaaccttt accgctgatt
     5221 cgtggaacaa atactctctt ccatccttaa ccggaggagg gaatatcctg cactcgcgta
     5281 cccatcgacg aactgtttca aggctccttg ggcgtcgctg gcgtgcgttc cactcctgaa
     5341 gtgtcaagta catcgcaaag tctccgcaat tactcgcaag ggagcgagta ttggtttatt
     5401 ttctccaaca aaaaaggagc cgaagctcct ttgatgatta aaattcgaat tgtctcgccc
     5461 gaaggctttt cagcattggt tttgcccgtt cgaaaagggc gcttgcttgg tcaagtcgtg
     5521 tcgcctccct tagcagtaca tctctgtttt tcgtcaccgt gtaaaaagtt tcaaacgcga
     5581 tgtcatacag cttgctcgtg tatgatgagt tcagttcctt caatatcggg tacagacgtt
     5641 tgcagatgtc ctgcgcattc tccatctgta cctgcatata gcaaaggagg atgatttctt
     5701 cgtctgtgaa ttgctgcgct ggttgcatgc tgcgaagttt cttttcgcac tcgatgaagt
     5761 atcggcgtat ctggcggcct ttttcgttac gctcgaccat cgccgtttct ttggctgtat
     5821 cgagggtgag gtgatagtct ttgctacggc gatcgcctcc acgtcctttg atccccctgt
     5881 tttggtgaac caaaatgtaa tcgacatcaa cggcaaagcc gtattctgtt atgcgatttt
     5941 taatccatgt agagaagtca cgacgtactt ccaaaaattc gtgtaaatca cgggcattaa
     6001 cgagaagagt tgtttcgttg gatatagtgc cgttgaatac ggggatgagt tgactggtca
     6061 tttttatgtc ctttgcattt gttcagatag ccctgtgttc agcagggcgg tcaggtactt
     6121 gaacaccgtg caaagtcggc ccgtatcctt aacctttcgg ctgtttttcg gtatacgcgc
     6181 tacccgacca tatctgaaaa atggacataa aaaatccgca tgactgacgg gtgcggtttc
     6241 cgctttgcaa ggtgtgttca gcaccatgaa gcggaatata gccccgttaa tgcggtgttg
     6301 tcaaatcatg taggccttat cttgctgtaa gccgcgccat tcgggctttt ccccacattt
     6361 ggggaaaact atcttgtcaa aatcatgcag cctttcgctc tccttcgatg agttcctcta
     6421 ttttgtgcac tccagcctcg ttgtaccgaa atgtttcaac ctgcttgtca ttgtgcgccg
     6481 atttgtcgat gaaccacttc ccgtactttt ctgttttgag catgtaggtg ttggctatgc
     6541 gtcctacctt gttggctgaa ataccgtagc gctggccgac ttccgtagca gagtagtagt
     6601 gctctgagat agctggcaga ggtacaacat cgcgaccaac aatcgggttg aacactccag
     6661 ctactgctgc ctgctttgat tctgaggaca gattcgggaa catattcacc agtcgatccg
     6721 ccacgttcaa agctatttcc atggctcgcg cacggtctag gctactttct ggttgcggta
     6781 ctggttgagg atggttggca accttttcgc gctgattgaa gtagccgtcc tccagttttt
     6841 cgaacacgtc ccatgcctga tccgtttcga gcatcttcgc gtgacgggcg gctccgcgct
     6901 ctgtccagag gatgaggtgg cgtgcatttg gggcactttt cacaaagtag tttaaagcta
     6961 ctttgtgttt tagcgcgcgc agctcgtcac caacgacctt gtagaagtgt accccctcaa
     7021 caaagcgcga cttgttttca tgatggtttt gccggatgcg cacaacatcc gttccgtaca
     7081 actgcgccaa tgtttggtgg gtagttgttt ttaaagtcag ctgctaacac atagcattgt
     7141 cgctctccca tttctggcat tcgctcacta cagcttatcc aggcatccgg agttaccggc
     7201 actggctggg cggtataaag cggtgttata tctgcccgaa aattacatgc tttatgcagc
     7261 cgcacccacc gttcgacttc tgctttgtca gaatacatac cagtgaacgt gttatattca
     7321 cggtcaattt gcgtgaaggt taccttccac gccaccggct ctgctgtcag cgatgccaga
     7381 gcaatttcat aagcacggcg ttcaacattg tctcgcacat ccaaactgct gatacgctct
     7441 ttgatttctt taatcagttc tttgtcgttc acttatgcct ccgattcccg gatgtcaccc
     7501 aaatcaacgc tacaatgcca accagtgcaa cgagcgcaaa actatcaagc atctccaacc
     7561 tctttaacgg taactgtctg tgcctccagt tctgctatgc gcttttctga ggcttcaagt
     7621 aacgcctgct tatcgcgtag cgcttcttcc agttcagcaa catggcattc actatcaata
     7681 aggttgttct ctgctgcttc cagctcaaca cgcaacttcc ctaccgttag cgcaatttcc
     7741 tcgttctcct gatcgcggct tttgatgtat tgcaggtttc tttcccgttc atccagtagt
     7801 gccagcgcaa catttggatt aaaagcagca ataaattcag cgtttgcata agcctgaa
//
LOCUS       DADMGC010000165         7857 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.165, whole genome shotgun sequence.
ACCESSION   DADMGC010000165 DADMGC010000000
VERSION     DADMGC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.165"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="JNZ73_003682"
     CDS             complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="JNZ73_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAY8733807.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            322..1953
                     /gene="dcuS"
                     /locus_tag="JNZ73_003683"
     CDS             322..1953
                     /gene="dcuS"
                     /locus_tag="JNZ73_003683"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HAY8733808.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1950..2669
                     /gene="dcuR"
                     /locus_tag="JNZ73_003684"
     CDS             1950..2669
                     /gene="dcuR"
                     /locus_tag="JNZ73_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HAY8733809.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYDFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3240..4580
                     /locus_tag="JNZ73_003685"
     CDS             3240..4580
                     /locus_tag="JNZ73_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY8733810.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQL
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4658..6304
                     /locus_tag="JNZ73_003686"
     CDS             4658..6304
                     /locus_tag="JNZ73_003686"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAY8733811.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAVIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6427..7056
                     /locus_tag="JNZ73_003687"
     CDS             6427..7056
                     /locus_tag="JNZ73_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HAY8733812.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7195..>7857)
                     /locus_tag="JNZ73_003688"
     CDS             complement(7195..>7857)
                     /locus_tag="JNZ73_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HAY8733813.1"
                     /translation="AIYYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRRI
                     LWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGTALFWVLQVIMVADTVDY
                     GEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPNVEQSTQALLGMQFI
                     MIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDV
                     KA"
BASE COUNT         2030 a         2008 c         1930 g         1889 t
ORIGIN      
        1 cgactttcgt ttgagattaa ataacttgcc gttgccacgt acgcaattgc cagaatgctg
       61 acatatcgca gtgttgaaat agacatcgat cttttcgccg gtataacagc gataaccccc
      121 gtccagtagc gcctgatcca tgaccttccc tctctcatca gtatgattat cgctataagc
      181 atagccccgg aatcttcata cagcactggc agtttccgtt gccagagtct cttcccctat
      241 atagccaatc atttttactc cctttgaatt acccgcctca tcagagataa tgcttaagaa
      301 atttgtcaca caaggaagct gatgagacat tcattgccct accacatatt acgcaaacgt
      361 ccgatgaaat tgagtaccac tgtgatctta atggtcagcg cggtactgtt ctcggtgcta
      421 ttggtggtgc atctgattta cttctcgcaa atcagtgata tgacgcgaga cgggctggcc
      481 aacaaggcac tggcagtggc gcgtaccctc gccgactcgc cggaaatccg tcagggcttg
      541 cagaaaaaac cgcaggagag tggcatccag gccatcgcgg aagccgtgcg caaacgcaac
      601 gatctgctgt ttattgtcgt taccgatatg caaagtcttc gctactcaca tcctgaagcc
      661 cagcgtattg gtcagccatt taaaggtgat gacatcctta atgcgctgaa tggcgaagaa
      721 aatgtcgcta tcaatcgcgg ttttctggcg caggctttac gcgtatttac ccccatctac
      781 gatgaaaatc ataaacaaat tggcgtggtg gcgatcggcc ttgagttaag ccgcgtgacc
      841 caacagatca atgacagtcg ctggagcatt atctggtcgg tattatttgg catgctggtc
      901 ggactgattg gcacctgcat tctggttaag gtactgaaaa aaatcctttt cggcctggaa
      961 ccctacgaaa tctccacgct gtttgagcaa cgccaggcca tgttgcagtc tatcaaagaa
     1021 ggcgtcgttg ccgtggacga tcgcggcgag gtcacgctga tcaacgatgc cgcacaagaa
     1081 ttgctgaatt accgtaagtc gcaggacgat gagaaactgt cgacgctcag ccactcatgg
     1141 tcacaggtgg tagatgtctc ggaagtgtta cgcgacggta cgccgcgccg cgacgaagag
     1201 attacgatta aagaccggct attactgatc aacaccgttc cggtgcgcag taatggcgtt
     1261 atcatcggtg ccatttcaac cttcagggac aaaactgaag ttcgtaaact gatgcagcga
     1321 ctcgacggtc tggtcaacta tgctgacgca cttcgtgaac gatcccacga atttatgaat
     1381 aaattgcatg tgattctcgg attattgcat ctgaagagtt ataagcagtt ggaagattac
     1441 attctcaaaa cagccaataa ctatcaggaa gagattggct ctctgctggg taagatcaaa
     1501 tctccggtta tcgctggttt tttaatcagc aagattaacc gcgcgaccga tttaggccat
     1561 acgctgattt taaacagtga aagccagctg ccagacagcg gcagtgagga ccaggtcgcg
     1621 acgctgatta ccacattggg aaatctgata gaaaacgcgc tggaagcatt agggccggaa
     1681 cccggtggcg aaattagcgt aacattgcac taccgtcacg gctggctgca ctgcgaagtt
     1741 aacgatgatg gaccggggat cgcacccgac aaaatcgatc acatttttga caaaggtgtc
     1801 tcgacaaaag gaagcgagcg aggcgtcggt ttagcacttg tcaaacaaca ggtagaaaat
     1861 ctcggcggca gcatcgccgt ggaatcggaa ccggggattt tcacacaatt ttttgtccag
     1921 ataccctggg acggggagag gtcgaacaga tgatcaatgt attaattatc gatgacgacg
     1981 caatggtcgc ggagctgaat cgccgatacg tagcacaaat cccaggcttt caatgctgtg
     2041 gaacagcctc gacgctggag aaagccaaag agattatctt caatagcgat gcgcctatcg
     2101 acctgatatt gctcgatatc tatatgcaaa aagagaacgg gctcgattta ctgcctgtcc
     2161 tgcataacgc gcgttgcaaa agtgatgtga ttgtcatctc ctccgcagcc gatgcggcaa
     2221 ccattaaaga ttcgctgcat tacggtgtcg tggattacct gatcaaaccc ttccaggctt
     2281 cccgctttga agaggcgctc accggctggc ggcaaaagaa aatggcgctg gaaaaacatc
     2341 agtattatga ccaggccgag cttgatcagc taatccacgg cagcagctcc aacgaacagg
     2401 atcctcgtcg cttgccaaaa ggcttaacgc cgcagacgtt acgcacgctg tgccagtgga
     2461 ttgacgcgca tcaggactat gacttttcaa ccgacgagct ggctaacgag gttaacatct
     2521 cgcgtgtttc ctgccgaaaa tacctcatct ggctggtcaa ctgccacatc ttgttcacca
     2581 gtatccatta tggcgtcacg gggcgtccgg tttaccgtta ccgtattcag gcagaacact
     2641 actcactgct gaaacaatat tgccaataac ggatcgctgg ttatctgtaa gtaataatta
     2701 ttaccgtaat gcaaaatacc cctggcacaa gttacatcgt taatcagtaa aacttgtgcc
     2761 agatcaaata taattatccc tccatcatcg ttaaaaatta atatctcttc agatgaacgg
     2821 tgtttttaat ttcaaaacga taacaaaagt taattaacta ttatgtcacc cgcattatgt
     2881 gtatttttac ccacaaatgg gtagatcaga ttaatctata aacctaatga tatctgccct
     2941 gagaacaaaa aatagaccga taaatatcaa taagataaca gcaaacaaaa cattaacatc
     3001 tgcgcagtac aaactataaa cccatcgcca gagagttttt ctctctgaaa aagccgctta
     3061 tcacagtgca taaatttgcc gctgctttaa tcagccaata ttcactgtga ggtatttgct
     3121 aaagccggta acgaccaaac ggatatttag tcaggctctg aaaacagttc atacaaaaca
     3181 gaacgtgact gtgatctatt cagcaaaaat ttaaatagga ttatcgcgag ggttcacaca
     3241 tgttatttac tatccaactt atcataatac taatatgtct gttttatggt gccagaaagg
     3301 gtggtatcgc gctgggttta ttaggcggta tcggtctggt cattctggtc ttcgtcttcc
     3361 accttcagct aggtaaacca ccagttgatg tcatgctggt tatcattgcg gtggtggcgg
     3421 catcggcgac cttgcaagct tcgggcggtc ttgatgtcat gctgcaaatt gccgagaagc
     3481 tgctgcgccg caacccgaaa tatgtctcaa ttgtcgcgcc gtttgtgacc tgtacactga
     3541 ccattctttg cggtacgggt catgtggttt acaccattct gccgatcatc tacgacgtcg
     3601 ccattaagaa caacatccgt ccggaacgtc cgatggcggc aagttctatc ggtgcacaga
     3661 tggggattat cgccagtccg gtgtcggttg cggtcgtgtc tctggttgcg atgctgggta
     3721 atgtaacctt tgatggtcgc catcttgagt tcctcgatct gctggcaatc accattccat
     3781 cgacgttaat cggtatcctg gcgatcggta tcttcagctg gttccgcggt aaagatctgg
     3841 ataaagacga agagttccag aaattcatct ccgtaccgga aaaccgtgag tatgtttacg
     3901 gtgataccgc gacgctgctg gataaaaaac tgccgaaaag caactggctg gcaatgtgga
     3961 ttttcctcgg ggcaatcgct gtagtcgccc ttcttggtgc tgattcggac ctgcgtccat
     4021 ccttcggcgg caaaccgctg tcgatggtac tggttattca gatgtttatg ctgctgaccg
     4081 gggcgctgat tattatcctg accaaaacca atcccgcgtc tatctcaaaa aacgaagtct
     4141 tccgttccgg tatgatcgcc atcgtggcgg tgtacggtat cgcatggatg gcagaaacca
     4201 tgttcggtgc gcatatgtct gaaattcagg gcgtactggg tgaaatggtg aaagagtatc
     4261 cgtgggcgta tgccattgtt ctgctgctgg tttccaagtt tgtaaactct caggctgcgg
     4321 cgctggcggc gattgttccg gtcgcgctgg cgatcggcgt tgatccggca tacatcgtgg
     4381 cttcagcacc ggcttgctac ggttattaca tcctgccgac ttatccgagc gatctggcag
     4441 cgattcagtt tgaccgttcc ggcaccaccc acatcggtcg cttcgtcatc aaccacagtt
     4501 ttattctgcc ggggttgatt ggtgtgagcg tatcgtgcgt cttcggctgg atcttcgccg
     4561 cgatgtacgg gttcttataa atgcactttg cgtgccgccc gtgactacgc ggcacgccat
     4621 tttcgaataa caaatacaga gttacaggct ggaagctatg tcaaacaaac cctttatcta
     4681 ccaggcacct ttcccgatgg ggaaagacaa taccgaatac tatctactca cttccgatta
     4741 cgttagcgtt gccgacttcg acggcgaaac catcctgaaa gtggaaccag aagccctgac
     4801 cctgctggcg cagcaagcct ttcacgacgc ttctttcatg ctccgtccgg cacaccagaa
     4861 acaggttgcg gctattcttc acgatccaga agccagcgaa aacgacaagt acgtggcact
     4921 gcaattctta agaaactccg aaatcgccgc caaaggcgtg ctgccgacct gccaggatac
     4981 cggcaccgcg gtcatcgtcg gtaaaaaagg ccagcgcgtg tggaccggtg gcggtgatga
     5041 agaagcgctg tcgaaaggcg tctataacac ctatatcgaa gataacctgc gctactcgca
     5101 aaacgcggcg ctggacatgt acaaagaggt caacaccggc actaacctgc ctgcgcaaat
     5161 cgacctgtac gcggtagatg gcgatgagta caaattcctt tgcgttgcga aaggcggcgg
     5221 ctctgccaac aaaacgtatc tctaccagga aaccaaagcc ctgctgactc ccggcaaact
     5281 gaaaaacttc ctcgtcgaga aaatgcgtac cctcggtact gcagcctgcc cgccgtacca
     5341 tatcgcgttt gtgattggcg gtacgtctgc ggaaaccaac ctgaaaaccg tcaaattagc
     5401 aagcgctcac tattacgatg aactgccgac ggaagggaac gaacatggtc aggcgttccg
     5461 cgatgtccag ctggaacagg aactgctgga agaggcccag aaactcggtc ttggcgcgca
     5521 gtttggcggt aaatacttcg cgcacgacat tcgcgttatc cgtctgccac gtcacggcgc
     5581 atcctgcccg gtaggcatgg gcgtctcctg ctccgctgac cgtaacatta aagcgaaaat
     5641 caaccgcgaa ggtatctgga tcgaaaaact ggaacacaat ctaggtcagt acattccaca
     5701 agaactgcgt caggccggtg aaggcgaagc ggtgaaggtc gaccttaacc gcccgatgaa
     5761 agagatcctc gcccagcttt cgcaatatcc ggtttccact cgtttgtcgc tcactggcac
     5821 cattattgtg ggtcgagata ttgcacacgc caagctgaaa gagctgattg acgccggtaa
     5881 agaacttccg cagtacatca aagatcaccc gatctactac gcgggtccgg cgaaaacccc
     5941 tgccggttat ccatcaggtt cacttggccc aaccaccgca ggtcgtatgg actcctatgt
     6001 ggatctgctg caatcccacg gcggcagcat gatcatgctg gcgaaaggca accgcagcca
     6061 gcaggttacc gacgcgtgtc ataaacacgg cggcttctac ctcggtagca tcggcggtcc
     6121 ggcggcggta ctggcgcagc agagcatcaa gcatctggaa tgcgtcgctt atccggagct
     6181 gggtatggaa gctatctgga aaattgaagt agaagatttc ccggcgttta tcctggtcga
     6241 tgacaaaggt aacgacttct tccagcaaat cgtcaacaaa cagtgcgcga actgcactaa
     6301 gtaacctctt cggcccagca cctggcagca tgctgccagg tgatccccct gggccacctc
     6361 ttttgcgatt gtaatttcat gcttgctggt gaatagtcag tattttccct gacttgcgaa
     6421 ctcaccatga cccgcacact caagccgtta attcttaaca ccggcgcact ggcgctaacg
     6481 ttaatcctga tttataccgg catttcggcc catgacaaac tcacctggct gctggaagtg
     6541 acaccggtga ttatcgtcgt gccgctacta cttgccaccg ccagacgtta cccgttaacg
     6601 ctgctgctct atacgctcat cttctttcac gccatcatcc tgatggtagg cgggcagtac
     6661 acctacgcga aagtccccgt tggttttgag gtgcaggaat ggctggggtt gagtcgtaat
     6721 ccgtatgaca agctggggca ctttttccag gggctggtgc ctgcactggt ggcacgagaa
     6781 attctcgttc gcgggatgta cgtgcgcgga cgtaaaatgg tggcgtttct ggtctgttgc
     6841 gtggcgctgg cgataagcgc gatgtatgaa ttaatcgagt ggtgggcggc actggcgatg
     6901 ggccagggag cggatgattt tctcggtacc cagggcgacc agtgggacac gcaatccgat
     6961 atgttctgcg cgctgcttgg cgcattaacg acagtgatat tgctcgctcg ctttcattgc
     7021 cgccagctac ggcgctatgg cttgataacc ggataacaac gtgcactttg ttgatgccgg
     7081 atgcggcgtg aattccttat ccggtctaca aaatttgcaa atccaaagca ttacattaag
     7141 acgtggcctg ataaacatat cgcatcaggc cattttcgtt agctattgtc cacgtcaggc
     7201 tttcacatca ctcactgcac cgaccggaat atcagcatga acaggctcgg gcggtacttt
     7261 gcgatattta tccagcagat gaatctggat cctgcgcagc gtgtcgccat tgaggcgata
     7321 gaaacggaag tagagaatca gcgttaccat gaaaaacaga gtcggtagag caatcataat
     7381 aaactgcata cctaataggg cttgcgtaga ctgttcaaca ttcggtacat agccaatcat
     7441 ccctaacaca accgcaatga aaaaagccgc aaaggctgaa ccgcccttca ccaccatagt
     7501 ctgcacggag taagcgatgc tttcacagcg tacgtgcagt ttatattcac cgtaatcaac
     7561 ggtatctgcc accatgatga cctgtaatac ccagaaaagc gccgttccca cattcagcaa
     7621 aatacccgca atcacaatga ggacgacgtt gtgatagctc attaatgcca ttaacaggag
     7681 aacaccacag cttaacaccg gaagaataga tgctccggcc cataaaatgc gtcgggataa
     7741 tgatttaact aagcgtggga agaatactaa cgtcaccagg ttagcagctc ccgcatacga
     7801 cagataatag gggaacaaat ccgcatcacc gataacatat gagaaataat agatagc
//
LOCUS       DADMGC010000166         7517 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.166, whole genome shotgun sequence.
ACCESSION   DADMGC010000166 DADMGC010000000
VERSION     DADMGC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.166"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            214..290
                     /locus_tag="JNZ73_003689"
     tRNA            214..290
                     /locus_tag="JNZ73_003689"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:248..250,aa:Arg,seq:tct)
     gene            complement(336..1097)
                     /locus_tag="JNZ73_003690"
     CDS             complement(336..1097)
                     /locus_tag="JNZ73_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8733814.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINYYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(1280..2170)
                     /locus_tag="JNZ73_003691"
     CDS             complement(1280..2170)
                     /locus_tag="JNZ73_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HAY8733815.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(2171..5149)
                     /gene="nfrA"
                     /locus_tag="JNZ73_003692"
     CDS             complement(2171..5149)
                     /gene="nfrA"
                     /locus_tag="JNZ73_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HAY8733816.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKAGDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTGAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPTNLPRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRELGNNAQYWWLHAQRYIPAQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDP
                     ALIRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVG
                     RRWTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLS
                     VYSRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTML
                     RASASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANG
                     QTIEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFK
                     AINQRNGERNNAFLTIGVHW"
     gene            complement(5136..7373)
                     /gene="nrfB"
                     /locus_tag="JNZ73_003693"
     CDS             complement(5136..7373)
                     /gene="nrfB"
                     /locus_tag="JNZ73_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HAY8733817.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVISNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHGAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQAQAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
BASE COUNT         1753 a         1949 c         1963 g         1852 t
ORIGIN      
        1 ttccgttagg gcggtaaatt gtactttgat acatgaaaat acgggttttc ttgattcaga
       61 cgcgcagcgg tgtgcgtttg tttgccgcta tagcgaaata aatcagaaaa tcagacgcgg
      121 tcgttcactt gttcagcaac cagaccaaaa gccattgact cagcaagggt tgaccgtata
      181 attcacgcga ttacaccgca ttgcggtatc aacgcgccct tagctcagtt ggatagagca
      241 acgaccttct aagtcgtggg ccgcaggttc gaatcctgca gggcgcgcca tttcttcttc
      301 atttatgccc gtcttatccg tttccgcttt gcccttcacc acatcacttt ttgtcgctgt
      361 ttggcgagat aattcaacgg tgtcaggctg taaaacgcct taaaaacaga gataaagtac
      421 gacgtgctgc tatagccaca taattgcgcc acctgagtga tatttttgtt atccatcaat
      481 aacatctgta cggcgtaacg catacgacac tctgtgacaa tctggctata gctggtattt
      541 tcgtttttta atttcttttt gagcaggctg gggcttaaac atagcgaact ggcgacaatt
      601 cgcagattcc agtaatgctg aatatcgctt tgaataatgc ggcagacgct gtcgcggacg
      661 ctgctgcgta agatatacat cagtagggca ataaaccgcg attgctcaag aaagttagac
      721 aatacggtaa aaagtaatgc gcgcgtcaac tccgcttcgc agggtttgct gggttgctgc
      781 ttgctgtggt tggcggctag ccggaacact tccggtgtac ggctatgaca agcgataacc
      841 ggggttgcca gccgcgacca gggctttaca caggttaatt tcttatttaa aaacaacaga
      901 taatagttga tgatgttacg gttgatgtgg gtgattaata cgtgatttaa cgttgagagt
      961 tcaatgacgt tattgttgca cgcgaaggcg gccaggtagt ttttcggaag ggtaaacgta
     1021 gcatggttat tgacgcttac ctctacctct ttttcggtca ggatcaccac gcaaggttca
     1081 ctgctgctca attgcatttc gcactcctca gatatcagaa actccgctca aaggatctat
     1141 gcttcctgca tgagtgatcg gcccgttcgc cgataacgat cttctttctt tagcatgctt
     1201 tttagcaatt aatcttgatg gaattctgac gagagcgaaa gaggtaagcc aggtcgtacc
     1261 cgacttacct ggaggggatt taatactcga gaatgccgtg cgcgacgggc aaatagcgca
     1321 gagagaaata gagcatgtct ttaccgcaag aggaacgttt cgctaacagc gaggcaattt
     1381 ctgcgtccgg tttcggtttc gctgtaaagg ttttgccttt gccggcgaca aacagttcat
     1441 aaacaatgcc gcttgctggg gcggagcttt ggcaatcggc gctggcctgt agataacgtt
     1501 cacgctgttc agcggtaagt ttatccacgc cgctgccatc ctgtacccag acattaactc
     1561 cggttgcttt aacttgttcc agcagttggc gatagccatc gggcgacatg tttccggcga
     1621 aaaaactact gatataaacc ggttttgctg aaacatcgct aatcagccgc tgcgcgttgt
     1681 ttaaccatgt tagcaaaggt tgacgggcgg cttcgctgcg ccagttcagg tcgtcaattt
     1741 ccgcgctgat gtaccagcca tccggcgtta tgccaggcgc ggcgctccat aatctggctt
     1801 gctggagatc ggcagccagc aggcgattaa gatagctttc cagcgctgca gacgactgtt
     1861 tctggtgcat aaaaaattcc ggatcggcgt tcagcccgac aatcagcttc aggccagtct
     1921 gttgcgcagc tgcggcacgc ttaaacaata acgtgcgctg ttctggctgg gtaaatgcat
     1981 cgccgtaacg ggtccattgc aaaaccaggg tatcgaagcc ttgaaaacgt aactgactca
     2041 tcagcccctg ccactgggta tcggtaacct gactatctcg gttttgtggt tgccagataa
     2101 tacctttcat cgcaaaggaa aaagggctga ccaaaagcag tgtcagcaat acgaaaatga
     2161 acttacgcat ttaccagtgc actccaatgg tgagaaacgc gttgttgcgc tctccgttac
     2221 gttgattaat cgccttaaag gtatgttgat actcgacgcc gagactgact ttgtgcggcc
     2281 aggcgtcgta gtgcgtctcg ccggtccaaa tattccagcg gaccccgaca ccgccaagct
     2341 gcgcgccctg agtgccttta tcacgatagc cgttgtcctg aacgtgagcg taaggctcaa
     2401 tagtctgtcc gttagctacc ttctgatgcc agctgacgcg ataatctgcc gtccacgcct
     2461 gaatatcctg gcggatatat tgcgccgcat cgaggtacag gttttgggca aaccagcctg
     2521 aaccgttcgg gtgccattcg tcgctgtatt tgccgccatt aaagaatgag gcgctggcgc
     2581 gcagcatggt atcggatgcg ccattttgcc cgttcagcgg caactgctgt tcgacggcga
     2641 ggaaaaagat ctgatcgcgc agcggcttcc agcgcagacc ggtgccggac atcggatttt
     2701 tcaccggcat catcaccccg ttttctccgg tatcggcaaa gacgcggcta taaactgaga
     2761 gcaggtcgcc ttccagcagc atattgcgtc cgatgcggta ctcggcttcc agttgtccgt
     2821 agctacgata gcttttccca ggcgctgcac cgccgacatt attgttagcg gtactcattg
     2881 cgccggaacg caagccgatg gaagaatcga aactgaacgt ccagcggcga ccgacctcct
     2941 catgcaaacg gcggaaattg aagcgttgct gattttgctc tggggtcagt ggggttatca
     3001 gcgcctgatt atcaatgtca tcaatcacca gccgggcgta gtgctgcgtc gcaggcatgt
     3061 catccagacg ctggttcacg taggccagtt gtcggatcag tgccggatcg tccggaagcc
     3121 ctttatgcgc ctgttcgagc atttcccgcg actgtgcgat atcaccgcta tcccacaagg
     3181 cgtaaccaag cgctgcctgg gtgttgctat tattcggttc cagttccagt tccagcgcgg
     3241 cgcgcaaatc actcaccgcg gccggaacat tatgacgttg gcgataaatt gtcgcccgcg
     3301 caacgtaagc gttggcagaa ggggcaatat tgattgagcg cgtgagatcg ttcagtgcga
     3361 gttctggctg agctgggata taacgttgcg catgcagcca ccagtattga gcattgtttc
     3421 ccagctcacg ttgttctgcc tgttgaagcc agcgatcgcg agccgcgcca tttcccgctg
     3481 cctgagcggt gttggcggcg gcgagcagat cctcattgct catgtcgtga agactgattt
     3541 tctgccaggc cgccagtgcg gtggcgtagt cctcaacctg atacgcctga taggctaccg
     3601 cacgatgttg ccaggcgctc ggttgtcgtt gttcggcctg aagccatgca tacaacgcca
     3661 caccgggtag cgtgtcccga taacactttg ccagacggtt ccaggcggca gcatcgtagg
     3721 aaggcgacat atcgcccagc aagcgaacta ttgccgggca attatctgca atacccggca
     3781 actgactttg ccactgacgt tgctccgcca gcggtaaggg tttcgataaa atcgccacct
     3841 tcgccggcgt tgctaggtaa ggatgacttt ccagcagaga cgccagtcgc gccattaaag
     3901 tctggctgac acgcgcatcg ccctggaaag gatagcgttg cagcaataaa tcggcagctt
     3961 cgcgtgactg ctcgttctgc atcagttgcc aggtcagttg atccaggcgg ggaagatttg
     4021 tcggttcttg ctgatacagc aatcgtgcca gacgcagaga ttcagccttg ttacgggtcg
     4081 ccacgctgac agcataacgc tcctcaagca tttcattggc ggggagggtg gcgagcagtt
     4141 tttgcgctgc gtcgtactga ccctctttta acagcaccgg tagcgtcgcg ccaacaacat
     4201 actggcggtt atcggcaaac tgcaccgtat aattcgccaa tgcctgaacg gggttggcgc
     4261 tgtatttaga taacagatag agccaacttt tctcttgtgc gcccgtggta aacagcggct
     4321 tattttcaat gagataatgc tggaggcgtg ctttttcgcc acgataagcc agcgcggtcg
     4381 cgtaagtaat atatgactga ggatcggtga agatcccctg tgattgcagt gccaggatcc
     4441 gatcgtccag ctgcccggca agaagcacgt caaaccactg acggcgttct gccgcgctta
     4501 atgtgttctg ctggcgtgct tcattgtata gcgtatctgc ctgggaccat tgtttcaggt
     4561 agattgcccg ttgcagcaga tcggttcgca gcgtttttcc ttccggcgat gcagcaaacg
     4621 tcgcatcgtt cagttgcgct ctggcgacag gtaattgtgc cagccgcagg gcattctgcc
     4681 cgacttcact gcgacaacgc agggtcggcg cagcgtcgcc cgctttttgc tgggcaagca
     4741 gttcttcaac agtcgtaacg cttttcactt caactggaat agccgccaga ctgcgctcaa
     4801 gtcgggcatc tcctgggtga cgtttcagtt gatcctcaag caacagccgc gcccggtcat
     4861 catgaccaaa atgacgatag gcttccgcaa ggtataaagt cagcggaata ttatccggca
     4921 cctgttggtg tatatattca aattcgcgga tggcggttgc ttcgtcgtta tttttctgtg
     4981 ccttcagcgc cttatcgaga cggggataaa taacaaaatg gcgataatcg ctcagcccca
     5041 gctcttctgc gctggtgccg atattgtctg cgagtgcgct ggtactcaat aaagacgtca
     5101 gcagtaaacc agaccatccg atgacgcgat taaggttatt ctccttcatt ttcggactcc
     5161 agttgcgcaa cctgttctgt gtttaaacct gctttgagta atagtgattg catcgaaact
     5221 tgtaattcgc gttgaattgt caggacgcga tccaacgttt cctggctgat aacgccttcg
     5281 gtgaccaaaa acttgccgag cggcagagaa ctgcgttcat ggcgcaataa caacacgtta
     5341 attgctgaac gattaatatg accgagcgtg gtcagtattt cggcgaacag gaactgatgc
     5401 ggcacatatt gccgccagat ttcaccggtc tgctgttccg tgagccactg atgctgaacc
     5461 gcattgtaca acattgcccg cggatcgtga ccgcgtcggc gtgcatacca gtggcgtaac
     5521 cctgtgacaa tttgtccccg cagaacaatg acgtaacgca ctttgcgtcc gactttacgc
     5581 gtcagggccg ccagcgaaac cgggtcaata ccatcttcac tgccgacaat taactcatca
     5641 ttttccagac gcagcggcag tactgcataa tgcagcgcta cggaggctgg catttcggca
     5701 atcagcgagg acgggatctg ccaggcatcg atggattccc acgccacgcc gttttgctct
     5761 gcctgcgcct gtgccagctg ctcggcgcta atcagcccct gcatcagcat tgaaccgccc
     5821 aggcgtagac cttcgacgcg attacgcagt gctgtatcga gttgttcttc agtgatgacc
     5881 tgattttcca gcagaatttg acctaacggg cgcaacgagc gggtatcgcc agtgacgctg
     5941 gggaagtcat gcgttgtttt atcccacgcc acgcgacgcg gatcgccgtg ctgaagaacc
     6001 tgtttcagca cgcgccagtt agccataaag ttaatcaggt tgccccagaa aagacgcagg
     6061 acggaaagca ggccctgcgt gaggccgtag tagccagtga cgaaaatcac tcgctgcacg
     6121 atgcggttaa ccatcaagcc aaagtttagc cacagcaggg tcattaacca tgcgctgccg
     6181 ctaaaaatag aaaggaaatg ccaggcatcg ggccacaaac tttcatacgc cagcaacagc
     6241 aaaagctgga tcatcaccag catcgcgagg aagctgacaa agttactgat tgcccctttg
     6301 cggtcgcgcc agagaaagta gttcagcgtc aggctggagg tccatttatg ggttttaaag
     6361 ccctggaaaa caatgccgat gatccagcga gatttttgtc gaaccgcagt cgaaaaggta
     6421 tcggggaaat attcgcgcac gcagatcatg tttgacgtcc gtgcgtgctg taaaaattta
     6481 cgctgctcgc gttctttggc ttcgtccacc accggaaaac ggacaaaaat ttccgtcata
     6541 cctttttctt tcaggcggaa gccaatgtcg taatcttcag taagactctg cacgtcgaaa
     6601 gcaataccgt taccgtcagc taacagtgcg gtcacggcgc ggcggctgaa acaggtgccg
     6661 acgcctgcgc tgggcacttg tccggcgagg gcttcacgca ccggaacatc tttgccatgc
     6721 agttctgaaa actcatcaat gtaagtcatg ctggtgaagt gcgtccattc gcgttcaaac
     6781 ggatacactg ggatctgaat cagatcttta cgctcgacca gatagttgaa cagacgcaat
     6841 tccatcggtg aaatcacatc ttcggcgcca tgcagaataa aaccagcaaa agcgaaattg
     6901 gcgctacgtt caaactgggt gatggcgtcc agcacgttgt tcagacagtc ggctttgctg
     6961 gtgggaccag gacgcgcgca gactacctta tgcacgttcg gaaagcgagc gcacacttcg
     7021 tcaacatcac gttgagtatc ggggtcgttg gggtaggtgc caacaaagat atgatagttt
     7081 tcgtagtcga gcgtggtcgc cgccagctcg gccatattgc tgatgacgcc cgtttcattc
     7141 cacgccggaa ccataatcgc taacggtttt tcatctggtt tatacagttc gcggtaactc
     7201 attcgcgggt agcggcgata aacactcaac ttgcgtttaa tgcggcgtac ccagtagacg
     7261 acatcaataa aaaaatcgtc cagcccgctg atgaacatga tgaccgctaa cgttatcgcg
     7321 attactttta agccgtagag ccaggtagca aaaacatcaa gaagccagtc cacacaaaaa
     7381 ccttacatta acgctggtta tgtttagggt ggcgtatatt aaggtttttt atgaattgtg
     7441 acagcttttt accattaata gggtaatgac tccaacttac tgatagtgtt ttatgttcag
     7501 ataatgcccg atgacct
//
LOCUS       DADMGC010000167         7471 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.167, whole genome shotgun sequence.
ACCESSION   DADMGC010000167 DADMGC010000000
VERSION     DADMGC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.167"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            15..1073
                     /locus_tag="JNZ73_003694"
     CDS             15..1073
                     /locus_tag="JNZ73_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY8733818.1"
                     /translation="MSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCD
                     TIVEAAALLPAFDQAVEGNRHQDDYGSGLGIAEEKFHYYLDLNDRYVYFYEPVNVEYF
                     AMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKG
                     IVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHD
                     LPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMT
                     GLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDNLGHQEGDLAITLHDAY
                     KASDERLYVNKQNKNSRS"
     gene            1116..1619
                     /gene="yeaK"
                     /locus_tag="JNZ73_003695"
     CDS             1116..1619
                     /gene="yeaK"
                     /locus_tag="JNZ73_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HAY8733819.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVIHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1620..1724)
                     /locus_tag="JNZ73_003696"
     CDS             complement(1620..1724)
                     /locus_tag="JNZ73_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733820.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1894..2340
                     /locus_tag="JNZ73_003697"
     CDS             1894..2340
                     /locus_tag="JNZ73_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAY8733821.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2297..3118)
                     /locus_tag="JNZ73_003698"
     CDS             complement(2297..3118)
                     /locus_tag="JNZ73_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8733822.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3215..4396
                     /gene="nimT"
                     /locus_tag="JNZ73_003699"
                     /pseudo
     CDS             3215..4396
                     /gene="nimT"
                     /locus_tag="JNZ73_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4451..4798
                     /locus_tag="JNZ73_003700"
     CDS             4451..4798
                     /locus_tag="JNZ73_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HAY8733823.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4820..5074)
                     /gene="yoaF"
                     /locus_tag="JNZ73_003701"
     CDS             complement(4820..5074)
                     /gene="yoaF"
                     /locus_tag="JNZ73_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HAY8733824.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5257..6282
                     /gene="dgcP"
                     /locus_tag="JNZ73_003702"
     CDS             5257..6282
                     /gene="dgcP"
                     /locus_tag="JNZ73_003702"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HAY8733825.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6315..6413
                     /gene="yoaK"
                     /locus_tag="JNZ73_003703"
     CDS             6315..6413
                     /gene="yoaK"
                     /locus_tag="JNZ73_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HAY8733826.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6416..6490
                     /gene="yoaJ"
                     /locus_tag="JNZ73_003704"
     CDS             6416..6490
                     /gene="yoaJ"
                     /locus_tag="JNZ73_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HAY8733827.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6542..6973)
                     /locus_tag="JNZ73_003705"
     CDS             complement(6542..6973)
                     /locus_tag="JNZ73_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733828.1"
                     /translation="MLTHWVTAQECVGMSCFPKHEVSIRRNLEKLAANHPELRRKRTG
                     SKALEFHISVLPAAARVELLLKSEQIETSQGYFEIARPTLEAHDYDREALWSKWDKAS
                     DSQRRLAEKWLPAVQTADEMLQPLLVRLSCYLSTGRLKVNA"
     gene            complement(6967..7194)
                     /locus_tag="JNZ73_003706"
     CDS             complement(6967..7194)
                     /locus_tag="JNZ73_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8733829.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSREAGLKPDTMRNVFRC
                     HIPRYEAIIARYLDMEPAQIWPSRYMEKTSC"
BASE COUNT         1834 a         1794 c         1805 g         2038 t
ORIGIN      
        1 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
       61 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      121 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      181 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatag
      241 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      301 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      361 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      421 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      481 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      541 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      601 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      661 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      721 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      781 tccggatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
      841 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
      901 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
      961 atgacaacct cggtcatcag gagggggatt tagcgattac gttacatgat gcgtataaag
     1021 cttccgatga gcgtttatat gtcaataagc agaacaaaaa cagccgttca tgataacctt
     1081 ctgtggttgt ttgcttgtaa tctcaggagc gtgaaatgac tgaaatggct aaaggaagcg
     1141 tgacgcatca gcagttaatc gctttattat cacaagaagg tgctaacttc cgcgtcgtta
     1201 tccatgaagc ggtaggtaaa tgtgaagcgg tgtcagaaat tcgtggcacc gctctaggcc
     1261 agggtgctaa ggcactggta tgtaaagtca aaggcaatgg cgtaaatcaa catgttctgg
     1321 cgatcctcgc agccgatcaa caggccgatc tgagccaact cgccagccat ataggcggat
     1381 tacgcgcttc gttggccagc ccggcggaag ttgatgaact cacaggctgt gtcttcggcg
     1441 cgatcccccc tttcagcttc catccaaaac tcaaactggt tgccgaccct ctactcttcg
     1501 aacgatttga tgaaatcgcc ttcaatgcag ggatgctgga taaatccgtt attttgaaaa
     1561 ccgcggatta tttgcgcatt gcgcaaccag aactggtcaa tttccgccgc actgcgtagc
     1621 tagcctatcc gttcaataag cagaacggac aaaaccagca caaccgcaat agcaaaaaac
     1681 gatgatgtga taatcagtgt ctcgacgaac atttgatcgt tcatagcatg cccccgggag
     1741 atagctgttt tcacgttatt gttagcgtgc acaaatggca gtttgatgac agttcgcctg
     1801 attttttatg caaaaaacgt aaagattttc tacttccttc ctgcagcaag cgtaaagtaa
     1861 gcaggcttat tattttttgg caaggaaacc acgatgtttg atgtcacttt gctgatcctg
     1921 ctcgggttag ctgcgctggg ctttatcagt cataacacca ctgtcgccgt ttcaattctg
     1981 gtgttgatca ttgtccgcgt cacaccgtta agcacctttt tcccgtggat tgaaaaacag
     2041 gggctaagta tcgggataat cattctgacg attggcgtta tggcccccat cgccagcggg
     2101 acgctaccgc cctcaacctt gattcactcg tttctgaact ggaaatcgct ggtggcaatt
     2161 gcagtaggcg ttattgtctc ctggctgggt gggcgcggcg tgacgttaat gggcagccag
     2221 ccgcaactgg ttgctgggct gctggtgggc actgttttag gcgtagcgct gtttcgcggc
     2281 gtaccggttg gcccgcttat tgccgcgggt ctggtttcgc tgattgtggg gaaacagtag
     2341 ttaatcccgc aatatatcgg cctggcgttt gccctaggcc tttcttaaac atagtaataa
     2401 aggccgttgt tgaatcatac cccagcgtat gagcaacttt ctgtacagta tcgcctttca
     2461 ctaatccctg aagcgccata atcagctgta actgctgacg ccactggcga aaactcaacc
     2521 ccgtctcttt cactatcagt cgggcaagat tgcgttcact catcgcaaaa aagccagccc
     2581 attgccccaa tgacccccac tcgacgggcc ctttcgccat catctccacc atcgtgcgga
     2641 ttttaggatg agaagaaacg ggcaggtgca gctgttgttg tggctgctga gggagttcat
     2701 caaacaggac ctgaattaat ctgcgggtca tcggctcagc acgctgagtc gtcgtcctgt
     2761 ttgccagggt taaaattaat tcccggcata agggcgatat tttcaatgtg caacatgttg
     2821 tcggcattgt cactgcgctg ggttcgataa acagaaaaca gagttcagcg ttagctgtca
     2881 cttgattgct atgcttcacc ccgcccggta tccaaaccgc atattgtggc ggcaccatcc
     2941 acaaagcatt ttccaccgta caggtaattg caccatgtaa tgctagtatc agctgtcctt
     3001 tgcggtgctg atgtacagga ctggttaact catccgttcc ggcgtgaata caaaatgcga
     3061 ccgcggcttc atggtgtcgg tcgggttcat agccattgag attcaacctg tgcatcattt
     3121 tgtccgaact tagcgataat ttgtcatttt agcttgattc aacataacaa taaaaacggt
     3181 aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt cattaagcgg
     3241 caaaaacagg attgtcctta tcgctggcat tctgatgatt gccacaacat tacgcgtcac
     3301 ctttaccggc gcagcgccgt tactggatac gattcgttcc gcttactcgc tgacgacagc
     3361 gcaaaccggc ttattaacca ccctgccatt attggccttt gcgctaatct cacctctggc
     3421 tgccccggtg gcgcgacgtt ttggtatgga acgtagcctg tttgccgcgt tacttttgat
     3481 ctgtgcaggt atcgcaattc gttctctccc ttcgccttac ttattatttg gcggtacagc
     3541 ggtcattggc ggtgggattg cattaggcaa tgtcttactg ccaggattaa ttaaacgcga
     3601 tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg gagctgcagc
     3661 ggcactggga tcggctatgg tcgtgccgct ggctttgaac ggttttggct ggcaaggcgc
     3721 gttgttcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc cacagtggcg
     3781 aagtcagcaa catgcaaatt tgagtacctc acgcgcctta catactcggg gtatctggcg
     3841 ttcgccactt gcctagcagg taacattgtt tcttgggatc aactcactgg tctattacgt
     3901 gattattggc tggcttccgg cgatcctcat cagtcacggc tatagcgaag cacaggcggg
     3961 ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga tcccactttt
     4021 cttacatcat gtgaaagacc agcgtggtat tgcagcgttc gttgctttga tgtgcgcagt
     4081 gggcgcggtt gggctctgct ttatgccagc gcacgcgatc acctggactc tgcttttcgg
     4141 ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtcttc gggcaagttc
     4201 tgcgcatcag gcggcggcac tctcggggat ggcacaatcc gtcgggtatt tgctggcagc
     4261 ctgtgggccg ccgctaatgg gtaaaataca cgatgctaac ggtaactggt ccgtaccact
     4321 gatgggtgtt gcaatacttt cactactgat ggcgattttc ggactttgcg ccgggagaga
     4381 caaagaaatt cgctaatatc cggtgctata gtgacgtgac aaatcttgcg tgaaagggag
     4441 aacaaacacg atgaatattc agtgcaaacg cgtttatgat ccggctgaac agagcgatgg
     4501 ttatcgcgta ctggtcgacc gcctctggcc gcgcggtatc aaaaaaaccg atttagccct
     4561 tgatgagtgg gataaagaaa tcacgccgtc aacggaactg cgcaaagcct ttcacggcga
     4621 agtcgtcgat tttgcaacct ttcgcgagca atatcgcgca gaactggcgc aacacgagca
     4681 agaaggaaag cggctggcgg acatcgccaa aaaacagccg ctgaccctgc tctacgcagc
     4741 aaaaaacacc acgcagaacc atgcgctggt gctggccgac tggctacgta gcttgtgatt
     4801 ttagtacggc atccggcggt tatttttcac cagccggatg gtcccgtcgc cacaatgccc
     4861 attcatcaat cgtttcaccg cccggtaatt tgcaattgtt gctgacccct tgcgctgtct
     4921 gcactggaat gagcgtcccg cccttctgct ggcaatagac cgacgccgga tttgccatac
     4981 caatctgcgg cggtttaggt gcttctggct gagaaggggt tgaacaacca gccaggacca
     5041 gcaagcaagg cagaacaaaa ctgataattt tcatttattg atctcacata tttatccaag
     5101 cttagagtat cgcggtatcg ttttgttttg cagcactatt tttattacat tcactcaaaa
     5161 catattacgt cttgtttcat ctttgttgat gatgttttat catgcctgca aagattaaat
     5221 aatcagcact tacccgccgt atcctggagt tgttccgtgt cagatcagat tatcgcccgc
     5281 gtctcgcaat cccttgccaa agaacagtca ctggaaagtc tggtccgaca gcttctggag
     5341 atgctggaaa tggtcactga tatggaatca acctacctga ccaaagtgga tgtcgaagcg
     5401 cgcctgcaac atattatgtt tgcccgtaac agccagaaaa tgcacatccc ggagaatttt
     5461 accgtctcgt gggattactc gttatgcaaa cgcgccattg atgaaaactg ctttttcagc
     5521 gatgaagtcc ctgaccgttg gggcgactgt attgcggcac gcaatcttgg catcaccaca
     5581 tttctgagca cgccaattca cttaccggat ggatcattct atggcacgct ttgcgccgcc
     5641 agcagtgaga agcgccagtg gagtgaacgc gcggaacagg ttttacagtt attcgccgga
     5701 ctgattgcac aatatattca aaaagaggca ctggttgaac agctgcacga agccaatgct
     5761 gcgctgattg cgcaatcgta taccgactcg ttaaccgggc taccgaatcg gcgggcgatt
     5821 tttgaaaatc tgacgacgct gttttccctc gcccggcatc ttaaccataa gataatgatc
     5881 gcgtttatcg atctggataa cttcaaatta atcaatgatc gttttggtca taatagtggc
     5941 gatctgtttc tcattcaggt tggcgagcgc cttaatacgc tccagcaaaa tggcgaagtt
     6001 attggtcgtc tcggtggtga tgagtttttg gttgtttcac taaacaacga gaatgcggat
     6061 atttcgtcgc tgcgagaacg tattcaacag caaatacgtg gagaatatca cttaggtgat
     6121 gttgatttgt attatcccgg tgccagtctt ggcatagtag aagtcgatcc tgaaacaacc
     6181 gatgcagaca gtgccctgca tgctgccgat attgcgatgt atcaggagaa aaaacacaaa
     6241 cagaaaacac cttttgtcgc gcatccagcg ctacattcct gaggcgtatt cacatccttt
     6301 tgattggtga taacatgcga atcggtatta tttttccggt tgtaatcttc attacagcgg
     6361 tcgtattttt agcatggttt tttattggcg gctatgctgc cccgggagca taaagatgaa
     6421 aaaaacaacg attattatga tgggtgtggc gattattgtc gtactcggca ctgagctggg
     6481 atggtggtaa cgtcacctct aaaaaatagc aaaggctgcc tgtgtgcagc ctttgtgcaa
     6541 tttaagcgtt aacttttaat cttcctgtag ataaatagca cgacaatcgc accaataacg
     6601 gctgaagcat ttcgtctgca gtctgaaccg caggcaacca tttttcagca agtctgcgct
     6661 gggaatcgct ggctttatcc catttgctcc acagtgcctc acgatcgtaa tcgtgcgctt
     6721 ccagcgtggg gcgggcgatt tcaaaatacc cctggcttgt ttctatttgc tcagatttta
     6781 ataacaactc aacacgtgct gccgctggta gaactgatat atgaaattcc aaagcctttg
     6841 aacccgtacg cttgcgacgt agttctggat gattggctgc aagcttttca agattgcggc
     6901 gaatactcac ctcatgttta ggaaagcatg acattcctac gcattcttgc gctgttaccc
     6961 aatgggttaa catgatgttt tctccatata ccgagaaggc caaatctggg ctggttccat
     7021 atccaaatat ctagcaatga ttgcttcgta tcgtggtata tggcagcgaa atacgttacg
     7081 catagtgtca ggctttaagc ctgcctctct ggataattga gatagtgtct tccccttcat
     7141 acgaacagca gcctttatct gctctgcgtg ccagtcttgg ttttttgctg acattctgcg
     7201 atccttactc tcttaacgtt ttcggtaact gataccggct accgctatcg ggaaaaacga
     7261 tatcgcagaa aaatgcgcat ttcaagagct atgcacattt ttattttaac caatgcaaat
     7321 caaagaattc tgtaaaattc tttatataac agagagattg agcatgacag gtgaaaaaga
     7381 aagcaaaaat gcagagcgca tttttatgcc ccggaaataa tgcgtttcaa ggaacgcctt
     7441 gaagaggcaa tgaatggaga atcaagccgg g
//
LOCUS       DADMGC010000168         7459 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.168, whole genome shotgun sequence.
ACCESSION   DADMGC010000168 DADMGC010000000
VERSION     DADMGC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.168"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..233)
                     /locus_tag="JNZ73_003707"
     CDS             complement(<1..233)
                     /locus_tag="JNZ73_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733830.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSFGFAPIFP"
     gene            753..1448
                     /gene="minC"
                     /locus_tag="JNZ73_003708"
     CDS             753..1448
                     /gene="minC"
                     /locus_tag="JNZ73_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAY8733831.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAGRL
                     QLVENALTVQPLN"
     gene            1472..2284
                     /gene="minD"
                     /locus_tag="JNZ73_003709"
     CDS             1472..2284
                     /gene="minD"
                     /locus_tag="JNZ73_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAY8733832.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2288..2554
                     /gene="minE"
                     /locus_tag="JNZ73_003710"
     CDS             2288..2554
                     /gene="minE"
                     /locus_tag="JNZ73_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAY8733833.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2926..3270)
                     /locus_tag="JNZ73_003711"
                     /pseudo
     CDS             complement(2926..3270)
                     /locus_tag="JNZ73_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3305..3424)
                     /locus_tag="JNZ73_003712"
     CDS             complement(3305..3424)
                     /locus_tag="JNZ73_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY8733834.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3385..3570)
                     /locus_tag="JNZ73_003713"
     CDS             complement(3385..3570)
                     /locus_tag="JNZ73_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733835.1"
                     /translation="MSRRYSSATITLVILFCRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3671..3844
                     /locus_tag="JNZ73_003714"
     CDS             3671..3844
                     /locus_tag="JNZ73_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733836.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3906..4190
                     /locus_tag="JNZ73_003715"
     CDS             3906..4190
                     /locus_tag="JNZ73_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HAY8733837.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVGAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            4194..4529
                     /locus_tag="JNZ73_003716"
     CDS             4194..4529
                     /locus_tag="JNZ73_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HAY8733838.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4586..6907)
                     /locus_tag="JNZ73_003717"
     CDS             complement(4586..6907)
                     /locus_tag="JNZ73_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY8733839.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINSQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LYLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPIPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(7024..7233)
                     /locus_tag="JNZ73_003718"
     CDS             complement(7024..7233)
                     /locus_tag="JNZ73_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733840.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
BASE COUNT         2047 a         1753 c         1654 g         2005 t
ORIGIN      
        1 tctggaaata taggggcaaa tccaaatgat tgtaagtttt cagcggcttt ttcgccgaga
       61 tatttccttg ttaactctgg ggaaatgcca tctgagttca cacaaatata cgggtcgcaa
      121 acaacgccgc acgccggaga ttgaagtccg ccagcgtcat gtccagacgc cattgatgag
      181 aacatcatcc ccacacctga caatccgata taaataatac gcatcatatt catagcacat
      241 cctgataatc caaccgtaca acacttatgc tcatttcgtc atcaatccgt aatcaaaacc
      301 gctgacctgg acgagttacg atgacctgac agaagtatca gacgtcttct gcggtttcta
      361 tgatagagca attctgtggc ggatattaag ataacgggta tctgttcttt aatgatgctg
      421 caaaaaatgg aaagttaaga aagcctggcg tggtgaaaaa atacacttgc ctcaatactg
      481 gatatttaaa caggtctgga gatgctgtca cgattagggt gcggaaaaag gaagcgtaaa
      541 taagaagaat caaaagtacg acggcaatgg gttgattgac aagggtattt tttaagctat
      601 gaatcagcgc catttatcac agaatagact tttactctga ataaatggga gggtgacttg
      661 cctcaatata atccagacta taacatgcct tatagtcttc ggaacatcat cgcgcgctgg
      721 cgatgattaa tagctaattg agtaaggcca ggatgtcaaa cacgccaatc gagcttaaag
      781 gcagtagctt cactttatct gtggttcatc tgcatgaggc agaacctaag gttatccatc
      841 aggcgctgaa agacaaaatc gctcaggccc ccacattttt aaaacatgcc cccgttgtac
      901 tcaacgtcag tgcactggaa gacccggtaa actggtcagc gatgcataag gcggtttcgg
      961 caaccggttt gcgggttatt ggcgtaagtg gctgcaaaga tgcgcaactt aaagccgaaa
     1021 ttgaaaaaat ggggctgcct atcctgacgg aaggaaaaga aaaagcgcca cgtccagctc
     1081 ccgcaccgca ggctccagcg caaaatacaa tgccggtcac aaaaacgcgt ttaatagata
     1141 ccccggtgcg ttccggtcag cgtatttatg ctccacaatg tgatctgatt gttacaagcc
     1201 acgttagcgc tggggccgaa ttgattgccg atgggaacat tcatgtctat ggcatgatgc
     1261 gcggtcgtgc gctggcaggg gcaagtggtg accgggaaac gcaaatattt tgtacgaacc
     1321 tgatggcgga attggtgtcc atcgcaggtg aatactggct gagtgatcaa atcccagcag
     1381 aattttatgg caaagcgggg cgactgcagt tagtcgaaaa cgctttgacc gttcaaccgt
     1441 taaattgatc cctttttaac aaggaatttc tatggcacgc attattgttg ttacttcggg
     1501 taaagggggt gttggtaaga caacctccag cgcggccatc gccactggtt tggcccagaa
     1561 gggaaagaaa actgtcgtga tagattttga tatcggcctg cgtaatctcg acctgattat
     1621 gggttgcgaa cgccgggtcg tttacgattt cgtcaacgtc attcagggcg atgcaacgct
     1681 aaatcaggcg ttaattaaag ataagcgtac tgaaaatctc tatattcttc cggcatcgca
     1741 aacacgcgat aaagatgccc tcacccgtga aggggtcgcc aaagttcttg atgatctgaa
     1801 agcgatggat tttgaattta tcgtttgtga ctccccggca gggattgaaa ccggtgcgtt
     1861 aatggcactc tattttgcag acgaagccat tattaccacc aacccggaag tctcctcagt
     1921 acgcgactct gaccgtattt taggcattct ggcgtcaaaa tcacgccgcg cagaaaatgg
     1981 cgaagagcct attaaagagc acctgctgtt aacgcgctat aacccaggcc gcgtaagcag
     2041 aggtgacatg ctgagcatgg aagatgtgct ggagatcctg cgcatcaaac tcgtcggcgt
     2101 gatcccagag gatcaatcag tattgcgcgc ctctaaccag ggtgaaccgg tcattctcga
     2161 cattaacgcc gatgcgggta aagcctacgc agataccgta gaacgtctgt tgggagaaga
     2221 acgtcctttc cgcttcattg aagaagagaa gaaaggcttc ctcaaacgct tgttcggagg
     2281 ataagttatg gcattactcg atttctttct ctcgcggaag aagaacacag ccaacattgc
     2341 aaaagaacgg ctgcagatta ttgttgctga acgccgtcgc agcgatgcag aaccgcatta
     2401 tctgccgcag ttgcgtaaag atattcttga ggtcatttgt aaatacgtac aaattgatcc
     2461 tgagatggta accgtacagc ttgagcaaaa agatggcgat atttctattc ttgagctgaa
     2521 cgtgacctta ccggaagcag aagagctgaa ataagcccgc tgtaaaaacg catttatctt
     2581 caaggcagag tcatttctgc cttgagtttt tcatccctct catccacgtt gtggtaaagc
     2641 ggcgagtatt cttgctgata ctcctcattg ctatttccac ctcccccttt acaccttaag
     2701 gctgttccca cgcacagaac aaaatgttta tgcctggttg agtaaataac catgttgtta
     2761 gtgtggatat gctgggagat gataaccccg caatttcatt attcattgtt ctcccatagg
     2821 atgaggtttc ccgccgtaac tggcgggctt tttttgcccc agaattttct ctttcaacat
     2881 cctgtaaacg aaaactgcgc cgaagcgcag tttaatcgtt gatggtcaga acgaccagtt
     2941 cacaccagcc accgcgttcc acggggactc cacaccggca ccatggctat accccacccc
     3001 gaaatgcccg cttaacgtac tgctgaatga ggctttaata cctgcctggt atattccacg
     3061 tctgcccgac aaatcattga caaaattacc gtcactattc actttcaccc gattatcatc
     3121 gacaaattct ttgcgcacag ccgccttcag gcagggttca atttccatac cgttccccag
     3181 acgcatgttg taactcagcg ttgcacccag ttcacgatat atactgcggg tatcgactga
     3241 tttcgattcc atgccattgg ataaatgata gtagcgtcta agtgacctaa gctctgctgc
     3301 aacatcagcc cgcaggcacc agaccagggg attcatcctg aagagacagc gcaggtgtat
     3361 tgtgttcacc gctcaccaaa gacatcatga tgaaatgatg atatcccgca taagaaagag
     3421 gcatttttta cacgcagtgc gctgaagtgt agttggataa aaaatcactc ttttcagata
     3481 atactgtcgt acgcttatct ttagacaatg aggccgtcgg caaaataaaa tgactaatgt
     3541 aatcgttgct gatgaatatc ttctgctcac ataaaaatca cacaataata ttgataaatc
     3601 agattatctt acctttacaa cattgcgtta acagtcaatg acacttcttg aataaaaata
     3661 ttgtcaatca atgagttatt cctcatttaa gattattctc attcacgtaa aaaacataat
     3721 tcctatcatc acagccacgc tcattttgaa ttatctgaat aatagcgagc gttctttagt
     3781 taagcagatc ttaattgaag acgaaattat tgtgtgtgca acttacttaa taccggacat
     3841 ctaagttgaa gaaaaaaata cttgccttcg ggcttatctc agcattgttc tgctccacgc
     3901 cagcgatggc ggatatgaac cgcaccacga aaggtgcatt gctaggcgca ggcgttggtt
     3961 tacttactgg caatggtgtt aatggcgtac tgaaaggtgc agctgttggc gctggtgttg
     4021 gtgcagtaac agaaaaaggc cgcgacggta aaaatgcgcg taaaggtgca aaagtgggtg
     4081 ccgccgttgg tgctgtcact ggcgttctga ccggcaatgg cctcgaaggg gcgattaaag
     4141 gtgccgtgat tggcggtacg ggtggtgcaa ttctgggtaa aatgaaataa ggaatgatga
     4201 tgaaaaaatt tgctttactg gcgggcctgt ttgttttcgc accaatgacc tgggcgcagg
     4261 actacaatat taaaaacggt ttgccgtcag aaacctatat tacttgcgca gaagccaacg
     4321 aaatggcaaa aactgacagc gcacaggttg cagaaattgt tgcggtaatg ggtaatgcca
     4381 gcgttgccag ccgtgattta aaaattgagc aatctccgga actgagcgcc aaagtggtag
     4441 aaaaactgaa tcaggtttgc gctaaagacc cgcaaatgct gttgatcacg gcaattgatg
     4501 acaccatgcg tgctatcggc aaaaaataat atttcatatg taccgcgtcg atgaatacga
     4561 cgcggttttt tagctaacgg cgggatcaga acttatattt caggcccacc atcactgcgg
     4621 tgtcattgta gccattatcg cccagctgca cgcccacatt cccccagacg ctggccgctg
     4681 gattcaggtt gccatttacg ccaagtttaa tttcccccaa attactcacc ccatcttgtt
     4741 cgactttcac accgttcatt gagacagcaa aatctttact gttatggatc cagttcattt
     4801 caacaaaagg ttcaaagtcg tcaccgtgcg gaccgctgtt tttctcctga gtacgaataa
     4861 aggtgcggaa gccgagtcgg gtctggatgt tgttatttgc gtcattctca atacgtgagc
     4921 cattggcttc cgtgtgcgca ttatgatcga cgcccatcca gatagcctgt gcctgtggct
     4981 ggaagatcca cgtatatttc gccgcttcca gaccaaagga tttattcagg gcaaaaccgt
     5041 aacccgcttc cagcgagacg gttgcacctt tcgcgctata ggattcatag gctaattcat
     5101 cccctttcac ggagttttta aaccagctat attgcgccca ggagtcaatg tatgcgcctt
     5161 ttttactgat gtcatcggca aaccaagtgg catacagccc tgcgctatag cctctgacac
     5221 tccctttcga acgataatcc gacacgctgg aatgagttaa gttgtagtcg cgggcataac
     5281 cagccatcac tcccaaacgc caactgtcac tatcggtaaa accaccggtt aacagatcgc
     5341 cccctaactg cgagacgtag cgatgcgagg tcgttctcaa ctgtccgttg ctgtcacgcc
     5401 aggcattatg cccgccaatt tgacgtagcc aaagtcggct tgaacgctcc tgttcagtta
     5461 caggatcgat gtagcgcgtt tcacccgcac ggtcgttcag acgcataaca aaaagagtgt
     5521 tagcggctgc caggttggcg acgtaacttc cagcttcggg gcgcaggacg gggataggcg
     5581 caacagtagg cgctgaactt cctccggagt ttgtttcacc gccgctcgtt gcctggaagt
     5641 tagtcagata ccagttttta ttggtcgctt cggtattacc tcgggttaat gaatattcgt
     5701 aggcaccggc gaaaatcctc gaatccttaa taaattcccc atttgattcc ccctcaacgc
     5761 tgataatctc tatcccattt aacgtgtaag ctccgctgcc tcctgcattg gtaactttaa
     5821 ctcgggttgt tccagaggta tttccattaa taacgacttt atccgtagct gaattatcat
     5881 catccaattc agttctgaga tacaatacgc cattattacc aacgtaattt tcagttattg
     5941 ttaatcttgt tggttcgaca tcccgtccat ctgcccggga aatatagacc gtactattat
     6001 caaccgtgag gttgctcacc gttgagtcag ctttaatatc ccaggtacta ttatctgaaa
     6061 gcgacagata ggtatgagtg ttatcatctg ttgaaatatt agctgaaccc gttatttttg
     6121 aattagttgc atcgacggtc aaatgacctt taatattatt gataaaaaca tctgcagccg
     6181 atgatacctc actatccgta atggtcacgt tactcgtctc atttttatgg tagaggattt
     6241 catttgctga ccgtagtgag ctaccagata aaatatctac cgttgcttcc tgactattta
     6301 tggaagactc cagcacaatt ccagacccct gatgcgcgac tgcagatgta tttttcagcg
     6361 tcaccgtgcc gccattataa attttagcaa tcgcggcgtc aggacccgtt gtataaattt
     6421 ctccctggtt aaatgaaact gtcccctggc gggcagcgat ggcatgagca ttgtccccat
     6481 gcgtggtgat atcaacatcg gttaatgtaa cgctggcatt ggtataatca agcgtgtcac
     6541 cagccttgac gacagtgcca ttagcgtacg cggcataact ttctgttccg tgggttgtga
     6601 tctccccttt tgccatatcc agttttgcta tggcattatt ttcattactt cctgtatcgg
     6661 cagtactttc ttcatcaaga gaagatacac acgcacgcgc gcttccggga gatgcacacg
     6721 caagaacgcc gactgcgtga ttacccgcag taacaataaa tgtatcatct ccgtcagaaa
     6781 aagagggggt aaccgaaccg tcagagttga ctcttattat ggtgtcagta ttcaggtttc
     6841 cactcgtttg acttagcaaa ccaattgagt aatcccccgt tgtgacaatc gtgtttttac
     6901 cctgcaaaat aacagcaccg ccaaattcac ttacgattgc gctagctgtt aatgctgggc
     6961 ttagaggact attgtcagaa ggatataaac cgcccgttgt tacaatgacg ccattgttaa
     7021 tggttaactt actaccctct cctgcaacgg atacccgtaa cttccattac ccgttgttat
     7081 aactagcgca ttatcaatat cttttacttt tccatctttt attgccacag catgtttata
     7141 ttcatcgtgc tgaataaact gttcagcata tgcattaccc accgccagag caataagtcc
     7201 caaactaaac ccggggagtt ttttcagttt catagattaa ccatccattc attggattaa
     7261 caaacattcc tggtcaaata acctatgagc tcagattacc aaaacggagg gtaaagacaa
     7321 aaacaaaagc acaaaaaatt taaatagata aacagcgaca atatataaat gataaataag
     7381 tcggcattat catttttcac cttattttca tgacattgat cactttgggg tgactatttt
     7441 gtttaacaaa taactcttc
//
LOCUS       DADMGC010000169         7412 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.169, whole genome shotgun sequence.
ACCESSION   DADMGC010000169 DADMGC010000000
VERSION     DADMGC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.169"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..1153
                     /gene="rbsA"
                     /locus_tag="JNZ73_003719"
     CDS             <1..1153
                     /gene="rbsA"
                     /locus_tag="JNZ73_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HAY8733841.1"
                     /translation="YVEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFESKV
                     IIMDEPTDALTDTETESLFRVIRELKSQGHGIVYISHRMKEIFEICDDVTVFRDGQFI
                     AEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLRKG
                     EILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDDLANGIVYISEDR
                     KRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQAIG
                     LLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIILVS
                     SEMPEVLGMSDRIIVMHEGHLCGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            1158..2117
                     /gene="rbsC"
                     /locus_tag="JNZ73_003720"
     CDS             1158..2117
                     /gene="rbsC"
                     /locus_tag="JNZ73_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY8733842.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIVGT
                     LIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2142..3019
                     /gene="rbsB"
                     /locus_tag="JNZ73_003721"
                     /pseudo
     CDS             2142..3019
                     /gene="rbsB"
                     /locus_tag="JNZ73_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3145..4074
                     /gene="rbsK"
                     /locus_tag="JNZ73_003722"
     CDS             3145..4074
                     /gene="rbsK"
                     /locus_tag="JNZ73_003722"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY8733843.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQLLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4078..5070
                     /gene="rbsR"
                     /locus_tag="JNZ73_003723"
     CDS             4078..5070
                     /gene="rbsR"
                     /locus_tag="JNZ73_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HAY8733844.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5036..6463)
                     /locus_tag="JNZ73_003724"
     CDS             complement(5036..6463)
                     /locus_tag="JNZ73_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY8733845.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVTRLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     NVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6486..7178)
                     /locus_tag="JNZ73_003725"
     CDS             complement(6486..7178)
                     /locus_tag="JNZ73_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAY8733846.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDIAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1876 a         1795 c         1955 g         1786 t
ORIGIN      
        1 gtatgtcgaa gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagtt
       61 agtgggcgat ctttccatcg gtgaccagca aatggttgaa atcgccaaag tgctgagctt
      121 tgagtcgaaa gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga
      181 atccctgttc cgcgtcatcc gcgagctgaa atcgcaaggc cacggtattg tctatatctc
      241 ccaccgcatg aaagaaatct tcgagatttg cgatgacgtt accgtttttc gtgatgggca
      301 atttattgct gagcgcgaag tggcatcact gaccgaagat tcgctgattg agatgatggt
      361 gggccgcaag ctggaagatc aatatccaca cctggacaaa gcgccaggag atatccgcct
      421 gaaagtcgat aatctctgcg gacctggcgt taacgatgtc tcttttactt tacgcaaagg
      481 cgaaattctt ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct
      541 ctacggcgca ctgccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac
      601 ccgttcaccg caggatgacc tggcaaacgg cattgtgtat atctccgaag accgtaaacg
      661 tgacggttta gtgctgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta
      721 cttcagccgc gctggcggca gtttgaagca tgccgatgaa cagcaggctg tgagtgattt
      781 cattcgtctg tttaatgtga aaacgccgtc gatggaacag gcaattggtc tgctttccgg
      841 tggcaatcag caaaaagtgg cgattgcccg cggtctgatg acacgcccca aagtattgat
      901 ccttgatgag cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat
      961 taaccagttc aaagccgatg gcttgagtat cattctggtg tcatcggaga tgccagaagt
     1021 attaggcatg agcgatcgca tcatcgtcat gcatgaaggg catctctgcg gggaatttac
     1081 tcgtgagcag gccacccagg aagtgttaat ggctgccgct gtgggcaagc ttaatcgcgt
     1141 gaatcaggag taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag
     1201 cgtggctgat ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga
     1261 cgttaagccc gaactttttc accatcaata acttattcaa tattctccag caaacttcgg
     1321 tgaacgccat tatggcggtc gggatgacgc tggtgatcct gacgtcgggc atagacttgt
     1381 cggtaggttc tctgttggcg ctaaccggcg cagttgctgc atctatcgtc ggcattgaag
     1441 tcaatgcgct ggtggctgtc gctgctgctc tcacgttagg tgccgcaatt ggtgcggtaa
     1501 ccggggtgat tgtagcgaaa ggtcgcgtcc aggcgtttat cgctacgctg gttatgatgc
     1561 ttttactgcg cggcgtgacc atggtttata ccaacggtag cccagtgaat accggcttta
     1621 ctgagaacgc cgatctgttt ggctggtttg gtattggtcg tccgctgggc gtaccgacgc
     1681 cagtctggat catggggatt gtcttcctcg cggcctggta catgctgcat cacacgcgtc
     1741 tggggcgtta catctacgcg ctgggcggca acgaagcggc aacgcgtctt tctggtatca
     1801 acgtcaataa aatcatcgtc tattctcttt gtggtctgct ggcatcgctg gccgggatca
     1861 ttgaagtggc gcgtctctcc tctgcacaac ccacggcggg gactggctat gagctggatg
     1921 ctattgctgc ggtggttctg ggcggtacga gtctggcggg cggaaaaggt cgcattgttg
     1981 ggacgttgat cggcgcatta attcttggct tccttaataa tggattgaat ttgttaggtg
     2041 tttcctccta ttaccagatg atcgtcaaag cggtggtgat tttgctggcg gtgctggtag
     2101 acaacaaaaa gcagtaataa cgactacagg acatcttgaa tatgaacatg aaaaaactgg
     2161 ctaccctggt ttccgctgtt gcgctaagcg ccaccgtcag tgcgaatgcg atggcaaaag
     2221 acaccatcgc gctggtggtc tccacgctta acaacccgtt ctttgtatcg ctgaaagatg
     2281 gcgcgcagaa agaggcggat aaacttggct ataacctggt ggtgctggac tcccagaaca
     2341 acccggcgaa agagctggcg aacgtgcagg acttaaccgt tcgcggcacc aaaattctgc
     2401 tgattaaccc gaccgactcc gacgcagtgg gtaatgctgt gaagatggct aaccaggcga
     2461 acattccggt tatcactctt gaccgccagg caacgaaagg tgaagtggtg agccacattg
     2521 catctgataa cgtactgggc ggcaaaatcg ccggtgatta catcgcgaag aaagcgggtg
     2581 aaggtgccaa agttatcgag ctgcaaggca ttgccggtac atccgcagcc cgtgaacgtg
     2641 gcgaaggctt ccagcaggcc gttgctgctc acaagtttaa tgttcttgcc agccagcaga
     2701 ttttgatcgc accaaaggtt tgaacgtaat gcagaacctg ttgaccgctc atccggatgt
     2761 tcaggctgta ttcgcgcaga atgatgaaat ggcgctgggc gcgctgcaaa ctgccggtaa
     2821 atcggatgtg atggtcgtcg gatttgacgg tacaccggat agcgaaaaag cggtgaatga
     2881 tggcaaacta gcagcgacta tcgctcagct acccgatcag attggcgcga aaggcgttga
     2941 aaccgcagat aaagtgctga aaggcgagaa agttcaggct aagtatccgg ttgatctgaa
     3001 actggttgtt aagcagtagt tttaatcagg ttgtatgacc tgatggtgac ataaatacgt
     3061 catcgacaga tgaacgtgta atataaagaa aagcagggca cgcgccaccc taacacggtg
     3121 gcgcatttta tggacatccc gaatatgcaa aacgcaggca gcctcgttgt tcttggcagc
     3181 attaatgctg accacattct taatcttcaa tcttttccta ctccaggcga aaccgtaacc
     3241 ggtaaccact atcaggttgc atttggcggc aaaggcgcga atcaggctgt ggctgctggg
     3301 cgtagcggtg cgaatatcgc gtttattgcc tgtactggcg atgacagcat tggcgagagc
     3361 gttcgccagc tgctcgccac tgataacatc gatattactc cggtcagcgt gatcaaaggc
     3421 gaatcaacag gtgtggcgct gatttttgtt aatggcgaag gtgagaatgt catcggtatt
     3481 catgccggcg ctaatgctgc tctttcccca tcgctggtgg aagcgcaacg tgagcgtatt
     3541 gccaacgcgt cagcgttatt aatgcagctg gaatcaccac tcgaaagtgt gatggcagcg
     3601 gcgaaaatcg cccatcaaaa taagactatc gttgcgctta acccggctcc ggctcgcgaa
     3661 cttcctgacg aactgctggc gctggtggac attattacgc caaacgaaac ggaagcagaa
     3721 aagctcaccg gtattcgtgt tgaaaatgat gaagatgcag cgaaggcggc gcaggtactg
     3781 catgaaaagg gtatccgtac tgtactgatt actttaggaa gtcgtggtgt atgggctagc
     3841 gtgaatggtg aaggtcagcg cgttcctgga ttccgggtgc aggctgtcga taccattgct
     3901 gccggagata cctttaacgg cgcgttaatc acggcgttgc tggaagaaaa accattgcca
     3961 gaggcgattc ggtttgccca tgctgccgct gcgattgccg taacacgtaa aggcgcacaa
     4021 ccttccgtac cgtggcgtga agagatcgac gcatttttag acaggcagag gtgacgcttg
     4081 gctacaatga aagatgttgc ccgtttggcg ggcgtttcta cctcaacagt ttctcacgtt
     4141 atcaataaag atcgcttcgt cagtgaagcg attaccgcca aagttgaagc ggcgattaaa
     4201 gaacttaatt acgcgccatc agctctggcg cgtagcctca aactcaatca aacacatacc
     4261 attggcatgt tgatcactgc cagtaccaat cctttctatt cagaactggt gcgtggcgtt
     4321 gaacgcagct gcttcgaacg cggttatagt ctcgtccttt gcaataccga aggcgatgaa
     4381 cagcggatga atcgcaatct ggaaacgctg atgcaaaaac gcgttgatgg cttactgtta
     4441 ctgtgtaccg aaacgcatca gccttcgcgt gaaatcatgc aacgttatcc gacagtgcct
     4501 accgtgatga tggactgggc tccgttcgat ggcgacagcg atcttattca ggataactcg
     4561 ttgctgggcg gagacttagc aacgcaatat ctgatcgata aaggtcatac ccgtatcgcc
     4621 tgtattaccg gtccgctgga taaaactccg gcgcgcctgc ggttggaagg ttatcgggcg
     4681 gcgatgaaac gtgcgggtct caacattcct gatggctatg aagtcactgg tgattttgaa
     4741 tttaacggcg ggtttgacgc catgcgccaa ctgttatcac atccgctgcg tcctcaggcc
     4801 gtctttaccg gaaatgacgc tatggctgtt ggcgtttacc aggcgttata tcaggcagag
     4861 ttacaggttc cgcaggatat cgcggtgatt ggctatgacg atatcgaact ggcaagcttt
     4921 atgacgccac cattaaccac tatccaccaa ccgaaagatg aactggggga gctggcgatt
     4981 gatgtactca tccatcggat aacccagccg acccttcagc aacaacgatt acaacttact
     5041 ccgattctga tggaacgcgg ttcggcatag atttacgctg tcttttgatc aaattattac
     5101 catcggttgt tttcagaagc atgaacattg ctgctgaagc aacagtaata atgcccatcg
     5161 tgataaacgt atagtggaat tgttcgacag tcgttgtgcc ttccatccct tcataaatgc
     5221 gaaggacggc cgcacttaca gcaacgccta aactaatcga cagttgctgc gtgaccgcca
     5281 gaacattgtt accgctgctg gcgttgtcat cggtcagatc ggcaagtgtg atggtattca
     5341 tcgcggtaaa ctgcgtcgac atagccatcc ctaatataaa caacggcaag atcagcatcc
     5401 atatcgccat tgccggtgat tgcaaagaga actgagcgat cattagccca ataatcaccg
     5461 tgatccccac taatgtatgg cgatagccca gacgacgtaa gacttgggta accatcgatt
     5521 ttgcaataat ggaacctaac gctgtcggtg ccatcataca gccggcaata aacgcctgat
     5581 aaccaaatcc tacctgtaac atcaatggca taaggaacgg tacacagccg gtccccagac
     5641 gggttgcaat attgcctacg ataccgatcg agaaagtgcg ggttttaaat aaatctaatg
     5701 aaattaatgg gtttggcgta cgtcgcgcat ggagaatata gagaagcagt aacccgatgc
     5761 tggtgacaat taccgtcaag gcaatccagc tggcgacaat cttttccccg aatagctcta
     5821 ttccgcttga aaagagaaca aggctgaggc caaacagcaa aaagccagtg atatcgaatc
     5881 tgcgtcgtgc ggtggtgaaa ttgggcatat gtttgcgcgc gtaaagaagg cccgcaatac
     5941 ctatggggat attaattaaa aatatccagt gccaggttgc ccaggtgacc agcacgccgc
     6001 caagaacggg gcctaaaatt ggccccacca gacccggcat ggcgacaaaa ttcaataccg
     6061 gaagaagttc attacgagga taagcacgca gtaaggccag ccgagtaaca ggcatcatca
     6121 ttgcgtcgcc tatcccttga ataacccgga agacaaccag ctgtggtagc gaattagaaa
     6181 gtgcgcaggc cagagaaccc aacgtgaaca gactcacggc aagggtaaaa atgcgacgcg
     6241 taccgaagcg atcggctagc catccgctta ccggaataag catcgccacc gtcagcgtat
     6301 aactgatgat ggctgattgc atcgcgagag gagaacgatt aaggctatga gcgattgcgg
     6361 gtaaggcggt attcagaata gtggcatcaa gtgcctgcat gaaaaaggcc atcgccgcga
     6421 tccacggcaa acccgacata ctacgcttct ttttatcgct cattcaatgt cctgttatcg
     6481 ggttatcact tatcaggtga gcgtagcagc gcctgacaag ctttaaatgc cgcgtcgcca
     6541 tcgctttgga taatcgcatc gacaatcgcc tggtgcagat ccagctttat cactgtgtcg
     6601 ctggtaattg acgtgaagta agtgtgataa accgaatgga atagcgaggc gaatgaggtc
     6661 aaaaacggat tggcgctcat ttcatagata tgctcatgcc aggctatatc gacttcgatc
     6721 cagcgttcac ggcgaaagtt ctcttttaat gccgccattt cggccattaa cgtattgaga
     6781 tgcgccttct gttccgcggt gccaaccgtt gctgccagta ggcaggcttg cggctccaga
     6841 cagatacgca taaccagaaa gtgatcgatg acctgatgaa agttctcttc tgtcatccac
     6901 caagtaagca attcctgatc aagaaaattc cagttcgatt gtggcatgac ccgagtacca
     6961 attcgcggtc gcggtaaaac catccctttt gccgttaacg ttttgaccgc ttcgcgtacc
     7021 gctgtacgac tcactccaaa ttgctcgccc agctcaatct caccgggcaa aatggtgccg
     7081 ggttcatatt caccttttaa gatccgttgc gccagcttct cagccagaac atacgaaagg
     7141 tttttctgtg cagctaactg ttgtgcgctt aaaggcatta cttatcttcc tttttctttt
     7201 tattcctcct tagtatgcca ccaggaagtg tgattacggt tgcaaaaacg gcaaattgat
     7261 tgttttatgg cacattaacg gggcttttgc tgaaaaaatg cgcggtcaga aaattatttt
     7321 aaatttcctc ttgtcaggcc ggaataactc cctataatgc gccaccactg acacggaaca
     7381 acggcaaaca cgccgccggg tcagcgggat tc
//
LOCUS       DADMGC010000170         7395 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.170, whole genome shotgun sequence.
ACCESSION   DADMGC010000170 DADMGC010000000
VERSION     DADMGC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.170"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(1..364)
                     /locus_tag="JNZ73_003726"
                     /pseudo
     CDS             complement(1..364)
                     /locus_tag="JNZ73_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599877.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            656..1396
                     /locus_tag="JNZ73_003727"
     CDS             656..1396
                     /locus_tag="JNZ73_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HAY8733847.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1367..2134)
                     /locus_tag="JNZ73_003728"
     CDS             complement(1367..2134)
                     /locus_tag="JNZ73_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAY8733848.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2340..2918)
                     /gene="lpcA"
                     /locus_tag="JNZ73_003729"
     CDS             complement(2340..2918)
                     /gene="lpcA"
                     /locus_tag="JNZ73_003729"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HAY8733849.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            3158..5602
                     /locus_tag="JNZ73_003730"
     CDS             3158..5602
                     /locus_tag="JNZ73_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAY8733850.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDTMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADSIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5645..6118)
                     /gene="ivy"
                     /locus_tag="JNZ73_003731"
     CDS             complement(5645..6118)
                     /gene="ivy"
                     /locus_tag="JNZ73_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HAY8733851.1"
                     /translation="MGRISSGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSAIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6272..7042
                     /gene="yafV"
                     /locus_tag="JNZ73_003732"
     CDS             6272..7042
                     /gene="yafV"
                     /locus_tag="JNZ73_003732"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HAY8733852.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            7151..7383
                     /locus_tag="JNZ73_003733"
                     /pseudo
     CDS             7151..7383
                     /locus_tag="JNZ73_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
BASE COUNT         1761 a         1854 c         2008 g         1772 t
ORIGIN      
        1 gccgtgcgcg ggagtttcgc ctcaaggatc ttgttgtagg cataaaaaac taacccacta
       61 caatcgaagc cttgatcggg ccgcgtgccg ccccagacgt aaggcttgcc aagctgctgc
      121 tccaggcggt gaatcgccac ttcggtgatg ttatgcaggt gatcgctgct taaaaagtgg
      181 ttgttttccg ccagctcctg ccagcgtctg tcactcgcct tgagcggcgc aggaaaccat
      241 tctggatgct gttttatctg ctcccggttg tggtggcgct ggcgagtaaa gggagcaaaa
      301 cgcataacga aaacacgcct ggatgcaaaa atctgccgag caagaacgaa gacatgaaag
      361 acataaaccg gctgaatgaa ggtgagaatt ttaaaagacc ggaggagtaa acgggattat
      421 taattgctta tttgcatatt actattgaga tttcataata ttaggaaata aatgatacat
      481 gcgcttttcg gactattgaa aactgcccct gtgctaaatt tgtaaaccca gcgccgacag
      541 gcttgcgtaa aactcttttg gtgataatgt gagccttcca aatccagact attccgattt
      601 gggagccatt ttgccgtcct ggcgtgtgta accgttttat caaggaataa gcagtatgcg
      661 taaaatcgca ttaattcttg cgatgttgtt gatcccgtgc gtttcgtttg ccggtttgct
      721 gggcagcagt agctcgacca cgccagtgag caaagagtat aagcagcagt tgatgggatc
      781 ccctgtctac atccagatct tcaaggaaga acgtacgctc gatctttacg tcaaaatggg
      841 cgagcaatat caactgctcg acagttataa aatctgtaaa tattccggcg gcttagggcc
      901 aaaacagcgt cagggcgatt tcaaaagccc ggaagggttt tatagcgtcc agcgtaatca
      961 gttaaaacca gacagccgtt actacaaagc gattaatatt ggattcccca atgcttatga
     1021 ccgtgcgcat ggttatgaag ggaaatacct aatgatccac ggcgattgtg tatccatcgg
     1081 ctgctacgca atgaccaatc agggtattga tgagatcttc cagttcgtta ctggtgcgct
     1141 ggtgtttggt cagccgagcg tgcaggtgag tatttaccca ttccgcatga ccgacgccaa
     1201 tatgaagcgc cataaatatt ccaactttaa ggacttctgg gagcagctga agccgggcta
     1261 cgactacttt gagcaaaccc gtaagccacc gaccgtttct gtggccaatg gccgttacgt
     1321 ggtcagcaag ccgttaagcc acgaagtggt gcagccacaa ctggcatcaa actacacgct
     1381 ccccgaggca aaataagcgc cattcgcctg gcataatttt ttgccaggtt tcattgcccg
     1441 tcagcggctg tgtcgcaatc accgtgacca catcattcgg tgtggtctgc gagctgaagt
     1501 cgatttccac atcctgatcc agcaacgttg ccacgccaaa cggcgcgcgg cgggtgatcc
     1561 agtgtaaatt agtcgagcaa tacgccatta catagcgccc gtccgaaagc agcatgttga
     1621 aaacgccctt cgcccgcagt tcatccgcca gtgaggcgat atatttaaat actgccgcca
     1681 tgttgcccgg cgtgcgcggg taacgctgcg ttaatttatg cagcagccag caaaaggctt
     1741 tttcgctgtc ggtttcaccg accgggcgga agttgccggt ttccagtgat ttgtagcccg
     1801 tcagttgtcc gttatgggca taagtccagt tacgccccca taactcgcgg gtaaatgggt
     1861 gagtattttc cagcgccacc tcgccccgat tagcctggcg aatatgagcc accaccgaac
     1921 aggattttat cgggtagtcc tggacaagtt tggcgatggg ggaattaaag ctgggttgtg
     1981 gatctttaaa tgtgcgacag cctttacctt cgtaaaaggt aatgccccag ccatctttat
     2041 gtggcccggt tccaccacca cgctgtacaa gtccggtgaa actaaagcag atatcggtag
     2101 ggacgttggc gctcatcccg agcaattcgc acatgggtaa cctccacaac agcgggagcg
     2161 catggacccc ggcgaagatt gatttaagaa ttttcaggtc ggatgcggcg taaacgaatt
     2221 attcggccta cggttcgggt acagatttgt aggcatgata agacgcgtca gcatcgcatc
     2281 aggcatcagc gcacaagtgc cggatgcggc gtaaacgcct tatccggcct acgccagact
     2341 tacttaacca tctctttttc aattaactgg atcaggatat ggatcacttt aatgtgaatc
     2401 tcctgaatgc ggtcggcata accaaagtgc ggaacgcgaa tttcgatatc cgccgtgcca
     2461 gccattttac cgccgtcttt accggtcagg gtgatcactt tcatcccctt ctcacgcgcc
     2521 gctgcgatcg ctttgatcac gtttgcagag ttaccggagg tggagatccc cagcagtaca
     2581 tcgccttcgc gacctaccgc ttcaacgtag cgggagaaaa tatcattgaa accgaaatca
     2641 ttaccgacgc aggaaatatg actaacgtca gaaatagcaa tcgccgggta gcccggacgg
     2701 ttttcacggt agcgaccggt caactcttcg gcaaagtgca tagcgtcgca atgggaaccg
     2761 ccgttgccgc aggaaagcac tttgccaccg gctttaaagc tgtctgctaa caggaccgcc
     2821 gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa agttagccag cgtttccgcc
     2881 gcttcgttca gttcgttacg aataagatcc tggtacatga ggatatcctt cagcataaat
     2941 gtaatagaca aaatgcagtg taccggatac cgccaaaagc gagaagtacg ggcaggtgct
     3001 atgaccagga ctttttgacc tgaagtgcgg ataaaaacag caacaatgtg agctttgttg
     3061 taattatatt gtaaacacat tgctaaatgt ttttacatcc actacaacca tatcatcaca
     3121 agtggtcaga cctcctacaa gtaaggggct tttcgttatg atgattttga gtattctcgc
     3181 tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg agcttattta tcagcagtct
     3241 gattttgctc gcctggacag ccgccctcgg cgttgctggt ctgtggtcgg cgtgggtact
     3301 ggtgcctctg accattatcc tcgtgccatt taactttgcg cctatgcgta agtcgatgat
     3361 ttccgcgccg gtatttcgcg gtttccgtaa ggtgatgccg ccgatgtcgc gcactgagaa
     3421 agaagcgatt gatgcgggca ccacctggtg ggagggcgac ttgttccagg gcaagccgga
     3481 ctggaaaaag ctgcataact atccgcagcc gcgcctgacc gctgaagagc aagcgtttct
     3541 cgacggcccg gtagaagaag cctgccggat ggcgaatgat ttccagatta ctcatgagct
     3601 ggcggatctg ccgccggagt tgtgggcgta ccttaaagag catcgcttct tcgcgatgat
     3661 tatcaaaaaa gagtacggcg gactggagtt ctcagcttat gcccagtctc gtgtgctgca
     3721 aaaactctct ggcgtgagcg ggatcctggc gattaccgtc ggcgtgccaa actcattagg
     3781 cccgggcgaa ctgttgcaac attacggcac tgacgagcag aaagatcact atctgccgcg
     3841 tctggcgcgt ggtcaggaga tcccctgctt tgcactgacc agcccggaag cgggttccga
     3901 tgcgggcgcg attccggaca ccgggattgt ctgcatgggc gaatggcagg gccagcaggt
     3961 gctggggatg cgtctgacct ggaacaaacg ctacattacg ctggcaccga ttgcgaccgt
     4021 gcttgggctg gcgtttaaac tctccgaccc ggaaaaatta ctcggcggtg cagaagattt
     4081 aggcattacc tgtgcgctga tcccaaccac cacgccgggc gtggaaattg gtcgtcgcca
     4141 cttcccgctg aacgtaccgt tccagaacgg accgacgcgc ggtaaagatg tcttcgtgcc
     4201 gatcgattac ataatcggcg ggccgaaaat ggccgggcaa ggctggcgga tgctggtgga
     4261 gtgcctctcg gtaggccgcg gcatcaccct gccttccaac tcaaccggcg gcgtgaaatc
     4321 ggtagcgctg gcaacgggcg cgtattctca cattcgccgt cagttcaaaa tctctatcgg
     4381 taagatggaa gggattgaag agccgctggc gcgtattgcc gggaacgctt atgtaatgga
     4441 tgctgcggca tcgctgatta cctacggcat tatgctcggc gaaaaacctg ccgtgctgtc
     4501 ggctatcgtt aagtatcact gtacccaccg cgggcagcag tcgattattg atacgatgga
     4561 tattaccggc ggtaaaggca ttatgctcgg gcaaagcaac ttcctggcgc gtgcttacca
     4621 gggcgcaccg attgccatca ccgttgaagg ggctaacatt ctgacccgca gcatgatgat
     4681 cttcggacaa ggagcgattc gttgccatcc gtacgtgctg gaagagatgg aagcggcgaa
     4741 gaacaatgac gtcaacgcgt tcgataaact gttgttcaaa catatcggtc acgtcggtag
     4801 caacaaagtt cgcagcttct ggctgggcct gacgcgcggt ttaaccagca gcacgccaac
     4861 cggcgatgcc actaaacgct actatcagca cctgaaccgc ctgagcgcca acctcgccct
     4921 gctttctgat gtctcgatgg cactgctggg cggcagcctg aaacgtcgtg agcgtatctc
     4981 ggcacgtctg ggggatattt taagccagct ttacctcgcc tctgccgttc tgaagcgtta
     5041 tgacgacgaa ggccgtaatg aagccgatct gccgctggtg cactggggcg tacaggatgc
     5101 gctgtatcag gcagaacagg cgatggacga tttactgcaa aacttcccga accgcgtggt
     5161 tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt cattatctgg caccgtctga
     5221 caagctggat cataaagtgg cgaagatttt acaagtgccg aacgccactc gttcacgtat
     5281 tggtcgcggt cagtacctga cgccaagcga gcataacccg gttggtttac tggaagaggc
     5341 gttggtggat gtgattgccg ccgactcgat tcatcagcgg atctgtaaag agctgggtaa
     5401 aaatctgcca tttacccgtc tggatgaact ggcgcacaac gcgctgacga aggggctgat
     5461 tgataaagat gaagccgcta ttctggtgaa agcggaagaa agccgtctgc gcagtattaa
     5521 cgttgatgac tttgatccgg aagagctggc gacgaagccg gtaaagttgc cggagaaagt
     5581 gcggaaagtt gaagccgcgt aattgaataa cggagccgaa aggctccgtt tctttatccg
     5641 ctaattattt aaaattaaag ccatccggat ggttttccag gctgccggtc aacgccgcga
     5701 acagcaccgt tttaccatca atcgaaagcg catcgttcac attcagccag gtgagtttct
     5761 cttgcgacgt tttctcatca atagccgaga acagccccgt catctgatta gatttctcgg
     5821 accacatcac agcgatacgt tgcgagccac agtcatgcgg tttgcacgcg ctcatcacct
     5881 gatacgtctc atctcccaac gttacggttt gtgcgggagt ataagtaccg cctttcatca
     5941 cccatgcagg tagcttatgc ccttgcacca tctgattaaa cgcagctttg gtggtttcgc
     6001 cctttgcaag gctgctaatg gttaaatcat cctgcgccat tgcactggtg gcgatgacca
     6061 gtgcggcgac tgtcgttatt gccttaaaca tcattcctcc cgagcttatc ctgcccatgt
     6121 tattaacctc ctaaagcctg gcgataacac gataatttgc aaatcagata tttccaatat
     6181 cagcatttgt tatcaattta ttctgcgcta aggcaactgc ctgataaggt gttaaagtta
     6241 taaaaccctt cgcataaagg agttgtacac cgtgcctagt ttgaagatta cgcttttgca
     6301 gcaaccgttg gtgtgggtgg atggtcctgc caacctgcgc cattttgatc gtcaactgga
     6361 aggtattacc gggcgcgatg tgatcgttct accggagatg tttaccagcg gctttgccat
     6421 ggaagcggca gcttcgtcgc ttgcacaaga tgacgtagtg aactggatga cagctaaggc
     6481 gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta caaacggagt ctggttcggt
     6541 taaccgcttt ttgctggttg agccgggcgg cacggtacat ttttatgata agcgtcatct
     6601 gttccgcatg gcagatgagc atctgcatta taaagcgggc aatgcgcgag tgattgtgga
     6661 atggcgcggc tggcgtattt tgccgctggt gtgctacgac ttacgttttc cagtgtggtc
     6721 gcgcaatctc aacgattatg acctcgccct gtacgtcgcc aactggccag ctccgcgctc
     6781 tctgcactgg caggcattgc tgacggcccg cgcgattgag aatcaggcgt atgtggcggg
     6841 atgcaatcgc gtcggcagcg atggcaacgg ctgccattat cgcggtgaca gccgggtgat
     6901 taatccgcaa ggcgagatta tcgctaccgc cgacgcgcat aaggcaacgc gtattgatgc
     6961 ggagctgtcg atggtggcat tgcgggaata tagagaaaag tttccggcat ggcgggatgc
     7021 ggatgagttt aggttgtggt gagtgggggt tgtgtttagg gccggggctg ggctagatta
     7081 cgaaagcccc taacttaaac acaatattac gccagaagtc accatttcca aaacacagcg
     7141 tcgctgtacc gtgggttatc tgcccaaccg cagcgacacg ccggtgatca aaatcagcgc
     7201 caaatggctg agaaaagcgg gatttgagat cggcacgggc gttaccgtga agatctcgga
     7261 gggatgtttg atcctgcttg ggataacaac gaagtacagg aactgcgcag ggaactttat
     7321 caggttaaac agtcagttaa ggggatgcga gacgggatgt ttagtgtgct gaacgaaagt
     7381 taaataataa aaagc
//
LOCUS       DADMGC010000171         7380 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.171, whole genome shotgun sequence.
ACCESSION   DADMGC010000171 DADMGC010000000
VERSION     DADMGC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.171"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            29..880
                     /gene="hchA"
                     /locus_tag="JNZ73_003734"
     CDS             29..880
                     /gene="hchA"
                     /locus_tag="JNZ73_003734"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HAY8733853.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(988..2346)
                     /locus_tag="JNZ73_003735"
     CDS             complement(988..2346)
                     /locus_tag="JNZ73_003735"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HAY8733854.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLESLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2346..3017)
                     /gene="hprR"
                     /locus_tag="JNZ73_003736"
     CDS             complement(2346..3017)
                     /gene="hprR"
                     /locus_tag="JNZ73_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HAY8733855.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3150..3563
                     /gene="hiuH"
                     /locus_tag="JNZ73_003737"
     CDS             3150..3563
                     /gene="hiuH"
                     /locus_tag="JNZ73_003737"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY8733856.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3672..4676
                     /gene="msrP"
                     /locus_tag="JNZ73_003738"
                     /pseudo
     CDS             3672..4676
                     /gene="msrP"
                     /locus_tag="JNZ73_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4677..5311
                     /gene="msrQ"
                     /locus_tag="JNZ73_003739"
                     /pseudo
     CDS             4677..5311
                     /gene="msrQ"
                     /locus_tag="JNZ73_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
     gene            5569..6219
                     /gene="zinT"
                     /locus_tag="JNZ73_003740"
     CDS             5569..6219
                     /gene="zinT"
                     /locus_tag="JNZ73_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAY8733857.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMISIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(7297..>7380)
                     /locus_tag="JNZ73_003741"
     CDS             complement(7297..>7380)
                     /locus_tag="JNZ73_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY8733858.1"
                     /translation="KKYRVLLNRVDTSTAPDIEWPEEPDTM"
BASE COUNT         2078 a         1680 c         1544 g         2078 t
ORIGIN      
        1 ctaactaagc aacaataagg aatacactat gactgttcaa acaagtaaaa acccgcaggt
       61 cgatattgct gaagataatg cgttcttccc ttcagaatat tcgcttagcc aatatacgag
      121 tcccgtctct gatcttgatg gcgtggacta tccaaaaccg tatcgcggta aacataaaat
      181 tctggtgatc gccgcggacg aacgttattt gccgaccgat aacggaaaac tgttctcgac
      241 cggtaaccat ccgattgaaa cgttgctgcc gttgtatcat ctccatgctg caggtttcga
      301 attcgaagtg gcgaccattt ccggtctgat gaccaagttt gaatactggg ctatgccgca
      361 caaagatgaa aaagtaatgc cattctttga acagcataaa tcgttattcc gtaatccgaa
      421 gaaactcgcg gatgttgtcg ccagcctcaa cgctgatagc gaatatgcag caatctttgt
      481 tcctggtggg catggcgcac ttattggttt accagaaagc caggacgtgg ctgccgcttt
      541 acagtgggca atcaaaaatg accgttttgt tatctccctt tgccacggtc cggcggcttt
      601 tctggcactt cgccacagcg ataacccact gaatggttat tctatttgcg cattcccaga
      661 cgccgcagac aaacaaacgc cagatattgg ctatatgccg ggtcatctca cctggtactt
      721 cggcgaagag ttgaagaaaa tgggcatgaa catcattaat gacgacatca ccgggcgagt
      781 acataaggac cgtaaacgtc tcaccggcga cagtcctttt gcagcgaatg cgttgggtaa
      841 actggcggcg caggaaatgc tggcagctta cgcgagttaa tccctgttac attgtgatgt
      901 aagcgcataa tcaatcgcag ccacgttaat actcacgacg tggctgcgtt ttttcacggt
      961 taacttatgg cgtactgaag cccgatgtta atttctttgc ggtaacgtaa ttcggaacac
     1021 gttatgctta ctgagatagt gatacgtagc acttcctcca tgtaattcgg caatcgcttt
     1081 gactaaagaa aggccaagtc cctgacctac ggaatgacgc gaattatctc cccgccaaaa
     1141 tctacggaag agtttttcag gctcattaat tttcgttcca ggactggcga tatcaatatt
     1201 aagagagccg ttggcatcaa gaaaactggt tatatgaata cgcgattttt ctggtgaata
     1261 tcgaatggcg ttaacaataa gattcgataa cattcgttgc agtaaaattt tatccgcaaa
     1321 gatttgttga ttgcacttga ccttaaagca aatctctttc tcgtctgaaa gagattcaag
     1381 atagtccaaa agattttcaa cttccttatt gagagaaagc gaatccagtt tcaccaaaac
     1441 attgtttttg tctgccctgg caagaaacag tatattctct gttaaccgcg aaatattttc
     1501 cagctcttca atgtttccgg caattgtttt ttgatattca gcgatacttc tgatttgact
     1561 gagcgtaacc tgattctgac ccagtaatgc attaattggc gttctaagtt catgagcgag
     1621 atcgtcagca aattgactaa ggcgctcaaa atctttgact aaagcatgat gcattttatt
     1681 caacgcctgc ccaagaggtt ttagttctct cggtaatgcg ttaacctcaa ccggcactcg
     1741 gctatcgtta taattcagcg cttccgttac gccactcaac tttttgatct ctcgcaaccc
     1801 cgtcctgatt aacaacggac ttaatactga gcacagtata atggcgacaa tgcaaattat
     1861 aatgctatta attttatact gttcaagcat attatgtctg gctgaagcca atttagccac
     1921 agtaaccgtt aatgatggcg aaacttcatc aatgttaatt cgtaaagcat ctatctctgt
     1981 atcattaata atgcttctgt aaatgcctgc tgcgctgatt gtctcactag caggtatgtt
     2041 atttaacatg tcatcactga catttgtccg gttaacaatt ttattgatgc catcactatg
     2101 aatgatcaaa atatcctgac taacatccat catccggtta aagtacacag gtaacgtatc
     2161 tggatttacc ccatcaatta acaactgctt gatctgcgct gtccggttaa tgagtgttgt
     2221 atcatcgcgc catttcaact cacttgccag gccattatag agagtccata caattccagc
     2281 gccagcaaca gactgcagaa atataaaaag caaggttaaa cggacggtta tagataatct
     2341 tttcattatt tttttaccgc tacgaatgaa tagcccatcc cccggattgt ggcgattagc
     2401 ttttcaggaa aaggatcatc aacttttgcg cggagcctgc gaatggcgac atccaccgta
     2461 ttggtatcac tatcaaagtt gattccccaa atttcactcg caataaccgt tctgggtata
     2521 atctcgccag ctctggaggc cagtagccaa agtaactgaa actccttgcg cgtcagtgta
     2581 atactgatat tgtccctgct gacactttga ctgacagagt ccatttttaa gccactgatt
     2641 tctaatgttg aattcagagc gtgatgttgc cttaattgtg cccgaaccct tgctaacaac
     2701 tcagaaaatg aaaaaggttt taccagataa tcatttgccc cactgtccaa ccctctgact
     2761 ctgtcatcga cagaatccct tgcagtaagg caaataacag gggtttgctt tgccgttctt
     2821 aacgtttgta agatctgcca gccatccata cccggaagca taatatccag aatgatcaat
     2881 gcataatcat ccttcagcgc aagataaagc ccatctctgc catcagaaac ggcatcaatg
     2941 acataacccg cttcggaaag cccctgcgtt acccattcct gggtcctttg attatcttca
     3001 ataagtagaa tcttcatgaa aataatatgc cataaatcat atgttataga aacaacctgg
     3061 ttcattacaa aattgtaatg ctgctgtaag gttaccctgg acgttttttc gctatcctca
     3121 aaactcattc acatgacaag gatataaaca tgttaaagcg ttatttagta ctctcggtag
     3181 caacggcagc attttcatta ccttctttgg tttatgccgc acaacaaaac attcttagcg
     3241 tgcacatttt gaaccagcaa acaggaaaac ccgctgccga cgtgacagtc actcttgaaa
     3301 agaaagcgga taacggctgg ttacaactta ataccgccaa aacagataag gatggacgaa
     3361 ttaaggcact gtggcccgag caaactgcga ctactggcga ttaccgtgtc gtatttaaaa
     3421 ccggggacta tttcaagaaa caaaatcttg aaagtttctt ccctgagatt cccgttgaat
     3481 ttcatattaa taaagtgaac gagcattatc atgtgccttt gttactcagc caatatgggt
     3541 attcaaccta tcgtggcagt taatgcgtaa atatgtaaaa gctgatctct gctactgtta
     3601 gggaggtgac tccctgacag attaacagta aacagctctt gttggttaac gacaaaaaag
     3661 tgtgatggct tatgaaaaag aatcaatttt taaaagaatc agatgttacg gccgagtcgg
     3721 tattctttat gaagcgtcgg caggtgttaa aagcactggg catcagcgca gctgcatttt
     3781 ctttgcctca cgctgcgcat gccgatctgc ttagctggtt taaagggaac gatcgtccgc
     3841 ccgcccccgc cggaaaaccg ctggagttca gcaagcctgc cgcctagcaa aataacctgc
     3901 cactgacgcc agcagataaa gtctccggtt ataacaactt ctatgaattc gggctggata
     3961 aagacgatcc cgcggctaat gctggtagcc tgaaaaccga tccatggaca ctgaaaatca
     4021 gcggcgaagt ggcaaaacca ttgaccctcg atcatgatga tttaacccgt cgcttcccgc
     4081 tggaagagcg tatttatcgt atgcgctgcg tggaagcgtg gtcgatggtg gtgccgtgga
     4141 ttggttttcc gctacacaaa ttgctggcgc ttgccgaacc caccagcaat gcgaagtatg
     4201 tcgctttcga aacgatttat gcgccggaac agatgcctgg ccagcaggac cgctttatcg
     4261 gcggcgggct gaaatatcct tatgtcgaag gattgcgtct cgacgaagca atgcatccgc
     4321 tcacactgat gaccgtaggt gtttatggca aggcgttacc accgcaaaat ggcgcgccgg
     4381 tgcgactgat tgtgccgtgg aaatatggct ttaaagggat taaatcgatc gtcagtatta
     4441 agctgacccg cgagcgtccg ccaaccacct ggaatctggc agcgcctgac gaatacggtt
     4501 tttacgccaa cgttaatccg catgttgatc acccgcgctg gtcacaggct accgaacgat
     4561 ttattggttc aggcggcatt ctcgatgttc agcgccagcc aacactactg tttaatggtt
     4621 acgccgacca ggtggcatcg ctgtatcgtg gcctggattt gcgggagaat ttctaaatgc
     4681 gtctgacagc aaaacaggtg atatggctga aagtttgcct gcatcttgcc ggattgttgc
     4741 cgtttctctg gctggtctgg gcgatcaatc acggtggact gggtgccgat ccggtgaaag
     4801 atattcagca ttttaccggt cgcactgcac tgaaattttt gcttgcggcc ttgttaatca
     4861 cacctctggc acgctacgca aaacagccgt tattgatacg cactcgccgc ctgttaggct
     4921 tatggtgctt tgcctgggcg acactgcatt taaccagtta cgcattgctg gagttgggcg
     4981 tgaataatct ggcgttatta ggtaaggagt taattacccg gccttattta acgttaggaa
     5041 ttatcagctg ggttattttg cttgctttag cgttcacttc aacccagtcg atgcagcgaa
     5101 aactcggcaa acactggcaa cagttgcata acttcgtcta tcttgtcgcg atcctggccc
     5161 caatacatta tctgtggtca gtgaagatta tttcaccgca gccactcatc tacgctgggc
     5221 tggctgtact gctttagcct tacggtataa gaagttgctt tcccttttca accggttacg
     5281 caagcaggcc cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat
     5341 cccggccgga tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt
     5401 tcgtttgtca tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca
     5461 atacacctga gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt
     5521 acaatataac attacacata atgtacatta actctggagg aaattgtttt ggcgattcgt
     5581 cttcataaac tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat
     5641 ggtcatcatt ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt
     5701 tttgatgata ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc
     5761 gtttaccctt tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca
     5821 gataaaacca aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat
     5881 atcgaaatga ttagcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca
     5941 tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc
     6001 gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt
     6061 agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat
     6121 tcccagcaat ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg
     6181 agtagcgaag aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt
     6241 tatttaacag aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga
     6301 caaactcaga cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac
     6361 tgagaaaaag gcgaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt
     6421 tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta attttgttgt
     6481 ttaaaccttt tttacttgtt agtataatac aaaggctttc aaaaaagctg ggaaattcaa
     6541 caactcttgg aagttatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt
     6601 ttggctcaat tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc
     6661 agtttcatag gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa
     6721 ctgtagattt tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc
     6781 acagtatcga acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct
     6841 gtttaggaaa agcaaggcgg gaagtcggga gataagtcat tgataaagtg gcggagagag
     6901 ggggatttga ccccccggta gagttgcccc tactccggtt ttcgagaccg atgatacaga
     6961 gtcgaaaaat caaagagtta aaaatttttc ttggaaaaaa tgtcttaaaa tcgtacacga
     7021 aaaatcaatg aattacataa cattaaagaa gaatattcca agccttcttg agtccaccac
     7081 ctgttttcag gtgtcattca gacgtgattt gccccagatt gggaaagcac gcttttttct
     7141 cagcttattc cgttttgcct gtgtgttgaa taattccact gcagttatat aaaatcggta
     7201 acggctggaa atcattcaat actcgcacta tcgaaagttt accagccaac cgcagcacgt
     7261 cctgcatacg tcgtgtctgc ggtttttctt tttcgcttac attgtgtctg gttcttccgg
     7321 ccactcaata tcaggtgcag ttgatgtatc aacacggttc agcaacaccc gatacttctt
//
LOCUS       DADMGC010000172         7288 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.172, whole genome shotgun sequence.
ACCESSION   DADMGC010000172 DADMGC010000000
VERSION     DADMGC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.172"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            91..432
                     /gene="yneK"
                     /locus_tag="JNZ73_003742"
                     /pseudo
     CDS             91..432
                     /gene="yneK"
                     /locus_tag="JNZ73_003742"
                     /inference="COORDINATES: protein motif:HMM:NF033404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            462..1202
                     /gene="yneK"
                     /locus_tag="JNZ73_003743"
     CDS             462..1202
                     /gene="yneK"
                     /locus_tag="JNZ73_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY8733859.1"
                     /translation="MFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQFPMINLGAPI
                     KRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKD
                     PNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQESPHFQDLFL
                     FFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKVTHE
                     DLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            1352..2542
                     /gene="ydeA"
                     /locus_tag="JNZ73_003744"
     CDS             1352..2542
                     /gene="ydeA"
                     /locus_tag="JNZ73_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HAY8733860.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2567..3232)
                     /gene="marC"
                     /locus_tag="JNZ73_003745"
     CDS             complement(2567..3232)
                     /gene="marC"
                     /locus_tag="JNZ73_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HAY8733861.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3444..3878
                     /gene="marR"
                     /locus_tag="JNZ73_003746"
     CDS             3444..3878
                     /gene="marR"
                     /locus_tag="JNZ73_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HAY8733862.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3898..4303
                     /gene="marA"
                     /locus_tag="JNZ73_003747"
                     /pseudo
     CDS             3898..4303
                     /gene="marA"
                     /locus_tag="JNZ73_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
     gene            4335..4553
                     /gene="marB"
                     /locus_tag="JNZ73_003748"
     CDS             4335..4553
                     /gene="marB"
                     /locus_tag="JNZ73_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HAY8733863.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4584..5483)
                     /gene="eamA"
                     /locus_tag="JNZ73_003749"
     CDS             complement(4584..5483)
                     /gene="eamA"
                     /locus_tag="JNZ73_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HAY8733864.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            5747..6865
                     /gene="ydeE"
                     /locus_tag="JNZ73_003750"
     CDS             5747..6865
                     /gene="ydeE"
                     /locus_tag="JNZ73_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HAY8733865.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            6992..7087
                     /gene="mgtS"
                     /locus_tag="JNZ73_003751"
     CDS             6992..7087
                     /gene="mgtS"
                     /locus_tag="JNZ73_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HAY8733866.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         1902 a         1774 c         1658 g         1954 t
ORIGIN      
        1 gcgccacgca tcacctgcct ttgatagttc ttgcagctca aatgccctat atagtgatgt
       61 tccgatgact tatgactata tggggcaaat atggttacgc cagtaagcat cagcaattac
      121 atatctcttc ctgatgattt tcccgctcgt aatattgcac ctcaagtaaa agaagtttta
      181 aaggatttta ttgatgcact cagtaccata atatgtgatg aagagtggcg cacgtcttta
      241 aacaccaact cagccacaaa aaagatattt aacaaccttg ataacttatc atatattcag
      301 aggacttctt ttcggggtaa cgacacacta tacaatgaaa aggttcagtt taaactcact
      361 tatcccgtca agaatggaag acacaaagaa aatattgaat ttcaggtagt aataaattta
      421 agccctattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
      481 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
      541 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
      601 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
      661 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
      721 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
      781 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
      841 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
      901 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
      961 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     1021 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactatttg
     1081 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     1141 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     1201 aaaccgtgat gttaccgact ctctgacgcg gaacagaatc agcgtcagag aaacggaaaa
     1261 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     1321 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     1381 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     1441 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     1501 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     1561 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     1621 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     1681 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     1741 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     1801 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     1861 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     1921 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     1981 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     2041 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     2101 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     2161 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     2221 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     2281 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     2341 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     2401 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     2461 ggtgcctgct tttgccgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     2521 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     2581 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     2641 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     2701 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     2761 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     2821 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     2881 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     2941 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     3001 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     3061 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     3121 cactgttcat gttgcctgcc aggccaagaa atagcgcaac agttgttaat gggttagcta
     3181 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     3241 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     3301 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     3361 gcatcggtca attcattcat ttgacttata cttgcctagg caatattatc ccctgcaact
     3421 aattacttgc cagggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     3481 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     3541 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     3601 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     3661 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     3721 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     3781 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     3841 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     3901 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     3961 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatgagcg
     4021 ttcgggttac tccaaatggc acctgcaacg gatgtttaaa aaagaaaccg gtcattcatt
     4081 aggccaatac atccgcagcc gtaagatgac ggaaatcgcg caaaagctga aggaaagtaa
     4141 cgagccgata ctctatctgg cagaacgata tggcttcgag tcgcaacaaa ctctgacccg
     4201 aaccttcaaa aattactttg atgttccgcc gcataaatac cggatgacca atatgcaggg
     4261 cgaatcgcgc tttttacatc cattaaatca ttacaacagc tagttgataa cgtgacaacg
     4321 tcactgaggc aatcatgaaa ccactttcat ccgcaatagc agctgcgctt attctctttt
     4381 ccgcgcaggg cgttgcggaa caaaccacgc agccagttgt tacttcttgt gccaatgtcg
     4441 tggttgttcc cccatcgcag gaacaaccac cgtttgattt aaatcacatg ggtactggca
     4501 gtgataagtc ggatgcgctc ggcgtgccct attataatca acacgctatg tagtttgttc
     4561 tggccccgac atctcggggc ttattaactt cccaccttta ccgctttacg ccaccgcaag
     4621 ccaaatacat tgatatacag cccggtcata atgagcaccg cacctaaaaa ttgcagaccc
     4681 gttaagcgtt catccaacaa tagtgccgca cttgccagtc cgactacggg caccagtaac
     4741 gataacggtg caacccgcca ggtttcatag cgtcccagta acgtccccca gatcccataa
     4801 ccaacaattg tcgccacaaa cgccagatac atcagcgaca agatggtggt catatcaata
     4861 gtaaccagac tgtgaatcat ggatgcggaa ccatcgagaa tcagcgaggc aacaaagaag
     4921 ggaatgattg ggattaaagc gctccagatt accagcgaca tcaccgccgg acgcgttgag
     4981 tgcgacatga tctttttatt gaagatgttg ccacacgccc aactaaatgc tgcagccagg
     5041 gtcaacataa agccgagcat cgccacatgc tgaccgttca gactatcttc gattaacacc
     5101 agtacgccaa aaatcgctaa ggcgatcccc gccaattgtt tgccatgcag tcgctccccg
     5161 aaagtaaacg cgccaagcac gatagtaaaa aacgcctgtg cctgtaacac cagcgaagcc
     5221 agtccagcag gcataccaaa gttaatggcg cagaacagaa aggcaaactg cgcaaaactg
     5281 atggttaatc cataccccaa gagcaaattc agtggtactt tcggtcgtgc aacaaaaaag
     5341 atggccggaa aagcgaccag cataaagcgc aaaccggcca gcatcagcgg tggcatgtta
     5401 tgaagcccca ctttgatgac cacaaaattt agcccccata cgaccactac cagtagcgcc
     5461 aacaccccat cttttcgcga cattctaccg cctctgaatt tcatcttttg taagcaatca
     5521 acttagctga atttactttt ctttaacagt tgattcgtta gtcgccggtt acgacggcat
     5581 taatgcgcaa ataagtcgct atacttcgga tttttgccat gcgtccgatg gttaataaca
     5641 acgacgaggc aagaagggcg ctggtagagc gtcgtaggga taagttcatt tgtctgtccg
     5701 gcagtgcgtt tcgttatgtt ttattttaga gatgtaaaga aatagcatgg ctacgctgcc
     5761 atttatgacc atttacttga gtcgccagta cagcctgagt gtcgatctaa tcggttatgc
     5821 gatgacaatt gcgctcacta ttggcgtcgt ttttagcctc ggttttggta tcctggcgga
     5881 taagttcgac aagaaacgct atatgttact ggcaattacc gccttcgcca gcggttttat
     5941 tgccattcct ttagtgaata acgtgacgct ggttgtgctc ttttttgccc tcattaactg
     6001 cgcctattct gtttttgcta ccgtgctgaa agcctggttt gccgacaatc tttcgtccac
     6061 cagcaaaacg aaaatcttct caatcaacta caccatgcta aacattggct ggaccatcgg
     6121 tccgccgctc ggcacgctgt tggtgatgca gagcatcaat ctgccctttt ggctggcagc
     6181 tatctgttcc gcgtttccca tgcttttcat tcaaatatgg gtaaagcgca gcgagaaaat
     6241 catcgccacg gaaacaggca gtgtctggtc gccgaaagtt ttattacaag ataaagcact
     6301 gttgtggttt acctgctctg gttttctggc ttcttttgta agcggcgcat ttgcttcatg
     6361 catttcacaa tatgtgatgg tgattgctga tggggatttt gccgaaaagg tggtcgcggt
     6421 tgttcttccg gtgaatgctg ccatggtggt tacgttgcaa tattccgtgg gccgccgact
     6481 taacccagct aacatccgcg cgctgatgac agcaggcacc ctctgtttcg tcatcggact
     6541 ggtcggtttt attttttccg gcaacaacct gctattgtgg ggtatgtcag ctgcggtatt
     6601 tactgtcggt gaaatcattt atgcgccggg cgagtatatg ttgattgacc atattgcgcc
     6661 gccaggaatg aaagccagct atttttccgc ccagtcttta ggctggcttg gtgccgcgat
     6721 taacccatta gtgagtggcg tagtgctaac cagcctgccg ccttcctcgc tgtttgtcat
     6781 cttagcgttg gtgatcattg ctgcgtgggt gctgatgtta aaagggattc gtgcaagacc
     6841 gtgggggcag cccgcgcttt gttgatttaa gtcgaacaca ataaaaattt aattcagcct
     6901 tcgtttaggt tatctctgct aatatctttc tcattgagat gaaaattaag gtaagcgagg
     6961 aaacacacca caccataaac ggaggcaaat aatgctgggt aatatgaatg tttttatggc
     7021 cgtactggga ataattttat tttctggttt tctggccgcg tatttcagcc acaaatggga
     7081 tgactaatga acggagataa tccctcacct aaccggcccc ttgttacagt tgtgtacaag
     7141 gggcctgatt tttatgacgg cgaaaaaaaa ccgccagtaa accggcggtg aatgcttgca
     7201 tggatagatt tgtgttttgc ttttacgcta acaggcattt tcctgcactg ataacggatc
     7261 gttgacacag tagcatcagt tttctcaa
//
LOCUS       DADMGC010000173         7061 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.173, whole genome shotgun sequence.
ACCESSION   DADMGC010000173 DADMGC010000000
VERSION     DADMGC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.173"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..92)
                     /locus_tag="JNZ73_003752"
     CDS             complement(<1..92)
                     /locus_tag="JNZ73_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleE"
                     /protein_id="HAY8733867.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRV"
     gene            148..300
                     /locus_tag="JNZ73_003753"
                     /pseudo
     CDS             148..300
                     /locus_tag="JNZ73_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1681..2628
                     /gene="icsP"
                     /locus_tag="JNZ73_003754"
     CDS             1681..2628
                     /gene="icsP"
                     /locus_tag="JNZ73_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HAY8733868.1"
                     /translation="MKSKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3190..3562)
                     /locus_tag="JNZ73_003755"
                     /pseudo
     CDS             complement(3190..3562)
                     /locus_tag="JNZ73_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3565..3762
                     /locus_tag="JNZ73_003756"
                     /pseudo
     CDS             3565..3762
                     /locus_tag="JNZ73_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3806..3925
                     /locus_tag="JNZ73_003757"
                     /pseudo
     CDS             3806..3925
                     /locus_tag="JNZ73_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4175..5041
                     /gene="ospB"
                     /locus_tag="JNZ73_003758"
     CDS             4175..5041
                     /gene="ospB"
                     /locus_tag="JNZ73_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HAY8733869.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5370..6110
                     /locus_tag="JNZ73_003759"
     CDS             5370..6110
                     /locus_tag="JNZ73_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY8733870.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(6463..>7061)
                     /gene="ospC4"
                     /locus_tag="JNZ73_003760"
     CDS             complement(6463..>7061)
                     /gene="ospC4"
                     /locus_tag="JNZ73_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HAY8733871.1"
                     /translation="LHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEYHIP
                     RMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGDKKADIALY
                     LLSKFNFTKQDIAEMEKMNNNIYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNKKF
                     QKANSGDTMLDNAMKSKDSKMIDFLLKNGAVSGKRFER"
BASE COUNT         2218 a         1318 c         1353 g         2172 t
ORIGIN      
        1 tcaactctag gataaatatt tctcactata tattcagtat ttgcagtatt tattggaaat
       61 acgttcttaa tattcttgat gggactaatc attttaatct ctatactcaa atataaacat
      121 taccatgaac aatataattg ttcctaagtg gaatgtctcc acggagaaca ctttatctat
      181 cgggaaatag tgcgggcaac agtgtttaat tatatcgagt gcgattacaa tcgctggcga
      241 cggcaccgtt ggtgtggcgg cctcagtccg gaacaatttg aaaaccagaa cctcgcttag
      301 gcctgtgtcc atattacgtg ggtaggctca cagagcctca cttacgactg cgtccatatt
      361 atgtgggtag gatcaataaa gacgttttat atgtgtctgc ttgcagccag aaaatttata
      421 ccctgcaggt atcataaaca tgcttgataa cttaattggg gctccctctt ccttttatgg
      481 tgtaattgtt tgattgaata cttccgggga tttcagtatg aaatgaagta tatttaatat
      541 actttacaaa aagtttgtat tagtggaata aggacaatag cttatccaaa ctgccaagcc
      601 tctttattat aagtaagatc tggcactgga atattctcaa aaggactaac atcatcacca
      661 cgaccttcaa ccaaccattt tattgcattt aaatcccaga atagttcagt agtggtcaat
      721 ttgggaaacg cacaatattg tacggttacg ttatcgcaat agagtgaact cccgccctat
      781 acggcgtttt caaggattag tccttaatcg gacaaccaca ttaaagcata ataaataatt
      841 aagctttaat aatatactat agatacagtt ctattataaa aaatactatt ttaggtgatg
      901 tattaaatta gtagaatatt tattctttta taaaagcacc aatgaattgt ccaagtaaac
      961 atgaggtaaa atgtgtagag gataactgag tttggtcact ttaactgtat tagtcgcagt
     1021 ctgactctcg actttaaaag gatgggaatt acctgtactg atagtgagaa agctttccat
     1081 tgaaataagt caaataaaag ttgatggctt cagtgctaaa gtgaggtgcc cgctagccgt
     1141 attcactcga atcggaattc aatgtattgc ttctgccatt tccataatat ttcacgggaa
     1201 aaacaaaatc tgaaaaacta tacttaagtg ttgtattaag tcagttgaga tacaaataca
     1261 tatttatcag cacacgttac gtcaaataca ggaggttgtc atgttggtac tgaaaggcac
     1321 gttggctatt gcagccgtga tgacaattta ttgtcttatc gttgttctta tggaacgctt
     1381 ttctgattga tttcatattg gcgagagaac aagaaggtga gtagaattgt cgcggaatcc
     1441 tgaaactatc agcctcaggt atgaacacca aaaggtaggt agagaaatct atggcccccg
     1501 ttactttatt cgcacaatga ggtatttttt tatttttaca aaagttcaat aaatattata
     1561 ataaaagata tgttcttggt aacataaaca aatatttaat taaaataatg cttggcattg
     1621 acgtttatct tataaagtaa gaagatcatg gacatttcaa ccaaaaaagt agagttctcg
     1681 atgaaatcaa aattccttgt acttgcactt tgtgtacctg cgatctttac tacacatgct
     1741 accactaact atccactttt cataccggac aacatcagta cggatattag tctaggatct
     1801 ctgagtggca aaacaaaaga acgcgtttat catcccaagg aaggggggag aaaaattagt
     1861 caactggact ggaaatacag taatgcgact attgttagag gtggcatcga ttggaagcta
     1921 attccaaaag tgtctttcgg agtttccggt tggactactt taggtaacca gaaagcaagc
     1981 atggttgata aagactggaa caattccaat acccctcagg tatggaccga ccagagctgg
     2041 catcccaata cgcatctccg tgatgctaac gaattcgagc tgaatcttaa aggttggtta
     2101 ttaaataatt tggattatcg acttggacta atagcaggtt accaagagag tcgttacagt
     2161 tttaatgcaa tgggagggag ttatatttat agtgaaaacg gggggaacag gaataaaaaa
     2221 ggggcacatc ctagtggtga aagaacaata ggttacaaac agctcttcaa aataccttat
     2281 attggattaa ctgctaatta ccgccatgag aattttgagt ttggagcaga actgaaatat
     2341 agtggttggg ttctttcatc tgatacagat aaacactatc agactgagac aatttttaaa
     2401 gatgaaataa aaaaccaaaa ttactgctct gttgctgcga atattggata ctatgtcacc
     2461 cccagtgcaa aattttatat agaaggctcc agaaattaca tttctaataa aaaaggtgat
     2521 acatctcttt atgagcaaag taccaatata tctggcacca ttaaaaatag tgcaagtatt
     2581 gaatatattg gttttctcac ttccgcaggt ataaagtata ttttttgaat tggttcaccg
     2641 agttgattac gttttcatgg ctaaaaattg ccgcccattg tgagtcatat tcgccctgac
     2701 tttccagaac catacgaact gcccgttgac ggacttcggg ggaaaaacgt gtatttttag
     2761 tcattctgtt tacctctttc tcagggcgtt tagtctccag gatttccggg gcggttcaaa
     2821 aaactcttcg ttgattactg tggacttact gttggcatta ccgaccctga gaccggagaa
     2881 ataagaaccg cttagatcat cgtggctgtt ctcggggtat caagttacac atgggcagag
     2941 gctacccaag gtctactatt atcgcctgaa agcgctgatg gaacagtgct atcagggaca
     3001 tgctgatgaa aatacctaaa taatcagcca gcactctatc tttccaaatc cacagcatag
     3061 caaagagagc aaaagagcct gtaaattcag aaattaagga aacgcctctt ggtgcacata
     3121 atctatctta tgttaaatga aatctaattt cttgattata tgctggatcg ttcgtgggtg
     3181 gctttgcccc tatatttcca gacacctgtt atcattggcc cgctgccgca gatattcccg
     3241 tggcgagcga taatccagcg cactatgcgg atgccattcg ttataatgct cgaatgcctc
     3301 tgcaaggttc tttgctgccg ttaacccgtc aggtttgggc atgatactga tgtagtcaca
     3361 ctttattgtt ttcacgaagc tctttgctat tctgttactc tccggactcc gcaccgccgt
     3421 gttcttcggt tcaacaaagt cccaacatcc gggtgaactg gcgtgtttca ttagcccggt
     3481 agcatgaacc attatccgtc agtcactcca ctggagacga cggaagatcg ttgccgaagc
     3541 ggtgttccac cgctcccagc atgtcgccaa tgggagagca tctacggtct gattgaactg
     3601 tttattcaaa ttgtccaccg tctgtcagta cgctctgaac gtcggctggt taaaacactg
     3661 ctcgctgatt ttcagagggt tcacggtaaa acagcacttt tattccggat tgcggaagcg
     3721 gctctgaata atccggatgg gctggttaaa gaggttgtgt accatctgca tattgttgaa
     3781 ccagatcgca gcggtaagag aagttcatca tacttggcgc agttacgcga tgtttctgct
     3841 agaggtgatg ccgtaaagaa cggcagaaca ttaccggaac aagatagcgg tctacctgca
     3901 ctggtatcag acccggggct tccccggatg atctccacgg tactttaggt actgtgtcta
     3961 tattatgtag gtaggatcaa ttaactttgt agttgaaatt ctttcattga aataatgaat
     4021 aaaaaagaca aaccgcatta aacaatgcca gaatataatt gcatttttgt tccgtttttt
     4081 taaatcatag tatacaaaga agcttgcata ctatatgaaa atataacacc ctttactatg
     4141 atgtttataa aacaatatat ggagtcatgt aggtatgaat ttagatggtg ttagaccata
     4201 ctgtagaata gtcaataaaa agaatgaaag catatcagat attgcatttg cacatataat
     4261 aaaaagggta aaaaattcat catgtactca cccaaaagca gcattggttt ttttaggaga
     4321 gaaaggtttt tgtgatagca atgatgttct atctattatg ggacaacaaa taccaagagt
     4381 atttaagaac aagatgttat atgattatgt ttttaaaaat gaaaaaagta aaaatgattt
     4441 tctaaaaatg gctgaatcat ggctaccaca gagtgaacca atagtaataa ataatgatga
     4501 tgacgcattg aatgctgctg cttatttttc tgtaaaaaaa gcgaaaataa aaacagtaaa
     4561 cgatactgat tttaaagagt ataataaggt ttatattctt gggcacggta gtcctggttc
     4621 tcatcaatta ggccttggtt cggaacttat tgatgtacaa acaatcattt caagaatgaa
     4681 agactgtggt attctaaatg tgaaagatat ccgttttact tcatgcggct ccgctgataa
     4741 agtggctcct aaaaatttta acaatgcccc tgctgaaagt ctttcttgta tccttaactc
     4801 tctgcctttt tttaaggaaa aagaatcttt gctagagcag ataaaaaaac accttgaaaa
     4861 cgatgagtca ttgagtgatg gtctaaaaat atccggctat catggatatg gagttcacta
     4921 tggtcaagag ctttttccct actcacatta tcgttcaact tcaattcctg ctgatccgga
     4981 gcatacagta aaaagaagct ctcagaaaaa gacttttatt attaataaag aactggatta
     5041 gtataaaatt tttaacctat agttaattgt ttgcatttaa gatgaagtta ttttatcttt
     5101 agtagaaaag tgcttcccta tgagggaaaa tcacaaatca tcataataca gagacaaaac
     5161 gatacgaaca aatagataaa aaacatcatt cttttaccac attatttttc gtgaatatga
     5221 aaaataatgt tattacttta atataagact attttttgtt tttccatcac tctgttcaaa
     5281 tttttccgca tgacttgtgt tttttgtaac acagctcgtt ttttacagct gaccaaaatc
     5341 atcaattaat tatgctaagg aaataaatta tgaaaaccaa aaactttctt cttttttgta
     5401 ttgctacaaa tatgattttt atcccctcag caaatgctct gaaggcagaa ggttttctca
     5461 ctcaacaaac ttcaccagac agtttgtcaa tacttccgcc gcctccggca gaggattcag
     5521 tagtatttca ggctgacaaa gctcattatg aattcggccg ctcgctccgg gatgctaatc
     5581 gtgtacgtct cgctagcgaa gatgcatact acgagaattt tggtcttgca ttttcagatg
     5641 cttatggcat ggatatttca agggaaaata ccccaatctt atatcagttg ttaacacaag
     5701 tactacagga tagccatgat tacgccgtgc gtaacgccaa agaatattat aaaagagttc
     5761 gtccattcgt tatttataaa gacgcaacct gtacacctga taaagatgag aaaatggcta
     5821 tcactggctc ttatccctct ggtcatgcat cctttggttg ggcagtagca ctgatacttg
     5881 cggagattaa tcctcaacgt aaagcggaaa tacttcgacg tggatatgag tttggagaaa
     5941 gtcgggtcat ctgcggtgcg cattggcaaa gcgatgtaga ggctgggcgt ttaatgggag
     6001 catcggttgt tgcagtactt cataatacac ctgaatttac caaaagcctt agcgaagcca
     6061 aaaaagagtt tgaagaatta aatactccta ccaatgaact gaccccataa agctggacag
     6121 cctgtatcag gctatggagg gcccatagac aaatctaccc tatatgagca taggaggagt
     6181 ctatgggcac accacgtttt actcctgaat ttaagggatt actggaaagg ctgggacata
     6241 tccctccggc agaagcagaa aaagcttatt atgctgccat cggaaacgat gatctggcaa
     6301 cctgagttca cagataaaac attctctagg aaactcgggg cggttccgtt caccacatgc
     6361 aatgtggtgt tgcaggggaa cggtctgcac catcccctat atcgatcaat ataacaaaaa
     6421 tgacaacttc agattcaggg cacaacctaa atatatttta ggtcacctct caaatcgttt
     6481 gcctgatacc gctccatttt ttaataaaaa atcaatcatt tttgaatctt tgcttttcat
     6541 tgcattgtca agcatagtgt ccccactatt tgctttttga aattttttgt ttacatcaac
     6601 caatccatta ttgataaaat attctagcac tttatagttg gcaccatctt tactaagtaa
     6661 gtattcaaca tcatacagat tacaatatat attgttgttc attttctcca tctctgcgat
     6721 atcttgtttt gtaaaattaa atttagatag taaataaagt gctatatcag cttttttatc
     6781 gcctaaagaa taagcaaggg cctgatgagc cattttttta tcagtcactt tattgttaat
     6841 ctcttcataa ttagatgctt taatcgcatc ctccaaagat atgtctctga gttttatttt
     6901 tttgaagtta tctgtactta gcattcttgg tatatgatac tcaagctgaa acacaaagtt
     6961 tataattcta tccagactaa caggatcaat gtttttgtta taacaaaact ctcggaatcc
     7021 ttgatcttta ctctttctta taaaatcaat aagatgcaac c
//
LOCUS       DADMGC010000174         6813 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.174, whole genome shotgun sequence.
ACCESSION   DADMGC010000174 DADMGC010000000
VERSION     DADMGC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6813
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.174"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..731
                     /locus_tag="JNZ73_003761"
     CDS             <1..731
                     /locus_tag="JNZ73_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY8733872.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPSVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMIQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIAWAITTQQKEYQA"
     gene            complement(1034..1804)
                     /locus_tag="JNZ73_003762"
     CDS             complement(1034..1804)
                     /locus_tag="JNZ73_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733873.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPLLPADGTQVSF
                     PYACEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1849..2283)
                     /locus_tag="JNZ73_003763"
                     /pseudo
     CDS             complement(1849..2283)
                     /locus_tag="JNZ73_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2295..2516)
                     /locus_tag="JNZ73_003764"
     CDS             complement(2295..2516)
                     /locus_tag="JNZ73_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733874.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2517..3200)
                     /locus_tag="JNZ73_003765"
     CDS             complement(2517..3200)
                     /locus_tag="JNZ73_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAY8733875.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3264..3575)
                     /locus_tag="JNZ73_003766"
     CDS             complement(3264..3575)
                     /locus_tag="JNZ73_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733876.1"
                     /translation="MKYDGDGRATARFFSDKGCRRVPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(3572..4543)
                     /locus_tag="JNZ73_003767"
     CDS             complement(3572..4543)
                     /locus_tag="JNZ73_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HAY8733877.1"
                     /translation="MRHGLMEAACERRIPMPNWCSNRMYFPGEPAQIAEIKRLASGAV
                     TPLYRRATNEGIQLFLAGSAGLLQITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTH
                     LQNGVLLDEQNCLMLHELWLQSGTGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNE
                     DVSVWWNRLCDNVVPEKTMPFDLLTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYG
                     VKWPCGYDLNISSQGENFIQVDFDTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFC
                     GWQLYERGELVDVLWGELEWSSPTDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4914..5873
                     /locus_tag="JNZ73_003768"
     CDS             4914..5873
                     /locus_tag="JNZ73_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY8733878.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5873..6268
                     /locus_tag="JNZ73_003769"
     CDS             5873..6268
                     /locus_tag="JNZ73_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY8733879.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6451..>6813
                     /locus_tag="JNZ73_003770"
     CDS             6451..>6813
                     /locus_tag="JNZ73_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAY8733880.1"
                     /translation="MTMATINARIDDDINNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAE"
BASE COUNT         1864 a         1760 c         1659 g         1530 t
ORIGIN      
        1 ttgatgcaga agccatatgt gaagcggctt cacgaccatc aatgcgtttt gtccagccaa
       61 gaactgagac ccagcaagca ataagggcat tacaccgggt cagagaatcg ctcatcagag
      121 ataaggtgaa aacaaccaac cagatccacg gtttcctgct ggaatttgga atcagcctgc
      181 cgacagggga tgccgtaata aaacggttgt ctctcgtatt ggctgaacac gaaatccccg
      241 aatatctgag ccgccttttg gtgaggttgc atacccacta cctttacctt gttgagcaaa
      301 tagcagagct ggaatcagaa ttaagtcagt caatcaacgc tgatgatacc gctcagcgca
      361 ttatgacaat tcctagtgtt gggccgatta ctgccagctt actatcgtcg caacttggtg
      421 acgggaaaca gttttcatgc agcagagatt ttgcggcttc cacagggctc gttccccggc
      481 agtatagcac tggaggaaaa tcgactctgt tgggtatcag taagcgaggt gataaaaatt
      541 tacgccggtt actggttcag tgtgcccgct cattcatgat acagcttgaa cgccagcatg
      601 gaaaacttgc tgagtgggtt cgggagcaac tcaacaagaa gcattcgaat gtggttgcct
      661 gcgctctggc aaataaactg gcccgaattg cctgggcaat cactactcag caaaaagaat
      721 atcaggcctg atatatagat gctgacaaaa ctcaattagc ttattgcaaa caataagata
      781 caagttaccg tctggttttg cgaagattga ttattgatga catgaacggc aaatcgacct
      841 gattaaaaac ctaacagggg aaaatggctc actgaagccg aggggatttt taggattatc
      901 aggtgcggaa ctcatcgagg cgcgggcatc ccttgcccac agagacgccg gatagattta
      961 agcaagccca ccattaatca aaaatcagtg ttgcaaaaac gggggtgacc atagattttc
     1021 cccgccatcg tcatcatttc cggatttttg ccagggcatc cgccatgctg cggatttcgg
     1081 cccgggcttc ccggacaact tcccgcagaa caccacggtc aaaccaccgg cggggcggtt
     1141 catccggtat acgcagcaca tcgcaggcca gcccactgga caggatatgc tcaatacaat
     1201 cgctgaccag atacatttcc accgccgaaa aacgtgcgcc ccggttcagg atggcatcga
     1261 gcaccacggg caggttttca tcgtcttcat cgagccagca ggggtgatcg ctttccttca
     1321 cgaccaggcg aaagcggcgc gtctggcggg tcagcaacgt ctgaccgctt gtggtcagcg
     1381 ccgcccggat acgccgcgca tcttctgccc cccggcaact gacactgcat actgcatcgc
     1441 gcaccgcatc aagaactgcc ctgatttcgt cttcggtcag ccattcacag gcatacggga
     1501 aagacacctg cgttccgtcc gccggaagta gcggcagcga ctgccactga ctgccggaca
     1561 gaacaagttc cgctccggtt ccggtcagtt tctgaatggc ggaggccagg tgcggcggca
     1621 gcacgccatc gtggcggtta acgagtaacg tcgccagccg gggcgcatcc agccgcacgt
     1681 aaatgctgat ccagcccgga tagttcgggt tacattggtc aacatccgtc tcttccggcc
     1741 agccctgcgc cagcagcagg ttacgggcgt gacgacaaat cagagcattc agcgtttcat
     1801 tcataattca tgaactccct gtatcgccgc ccgcaggcgg cgataacatc agtcgttttc
     1861 gtgtgtgttg tttttcagca ggctgcgggc ctgatcaacc atcgcctgtc ggcgggtaac
     1921 gtattccggg agggggaaac cgtgatcccg ggcagcccat tcacgtttcc acagtgcctg
     1981 ttcagcgatc tgagtcaggg tctccagcag ttcagcaggg acggtcacct ggcggttttc
     2041 gcgcacgttg ctgataatct ccatcgccgc ggcggaagaa ataccggact caatccgcta
     2101 gtcttccggg atgccctcca ggtgcgccag gacggtcctg atttcatgat cggacaggga
     2161 aatatccatt ccgtcagcga gggcctgaat ctcttccggg gaccagataa ccagcatcag
     2221 cggcgtgtct cccgaatact ttgctgccag cgcccggcat agcgtttcgc atgttccgta
     2281 catactctct cccctcaatc tttgaagcag gaatccagtt ccagaatttc ggtatatgcc
     2341 tccatcaaga ggcggtttgc gcgaaacaac gtctgacgca ccagacgttc cggcagggtg
     2401 tcagcattaa agctgtaccg ccgctccatc tcattcatgg ttgtacgcag gtcgcacatg
     2461 gcattggcca cctccgccac ttcggcgcga agtttttcgt gtccggcata gttcatttac
     2521 gcagcctccg gcataaacca gtcatcattc gcggccccct gctgcatggc ccgctgtacg
     2581 gcagccagtc gttgctgtcc ggcacggtga tactgctcaa gcagctcgat accgatatac
     2641 cggcgtccgg actggagggc ggcgacgcag gttgaaccgc tgcctgcaaa cgggtccagc
     2701 actatcgcat tcgggtgtgt gaagctctca atcagcggtt gcaggctggt aacgggcttt
     2761 tccgtcgggt gatggcggtt gcccgaatat ttccagccca acacgtccgg cagcgggttt
     2821 tgtggcagac gtggacggcc ttttgccagg atgtaggcgc attcgtggcg atagcccaca
     2881 tatgcggcct tcgatgtgta gtttttggtg aagaccaggt gaccaacaac gctgaatccc
     2941 gcatttttcc aggcagacat aaagcgatcg acgcggttcc agccatagaa gctcaccatc
     3001 agcgcgtctt ttttgagtac gcggtacatt tcattacagg ccggttgcag ccattcatca
     3061 gttttatcac cagcaatggt acgcccctga cggtcacgaa aaccgacgag atatggcgga
     3121 tcggtgagga tgaaatcaat ggcgttatca ggaatacggg ccataacatc gatgcagtta
     3181 ccgaggacaa aacgggacat aacagatcct tctcttacag agggcacccc tgtggtgccc
     3241 gttccccgct gcctgtgggc tgttcatcgc gtccggtaac gggtcaggcc gaccgtggaa
     3301 taccgcagcc gtcgtctttt tgacagcaag gttatgccgc gaaagcggct tgaagggcgt
     3361 tcatcagaaa agggcgacgg gctggcgtac actgtcaccg acgccagccg ggagcggtaa
     3421 aagcgcccgt cccgtgcccg ccggacgcgg gacactgacc acaggcagcg tttatgccgc
     3481 gccgcgtcag ccggcgcagt aaaaagagga acacggcgac atcctttgtc ggaaaagaaa
     3541 cgggcggttg cccgcccgtc tccgtcatac ttcatccgcc ataatgcgcc acattgtcga
     3601 ctatccacgc aggtccggtg acttccggct gctcatcgtc atctgtcggg gaagaccatt
     3661 caagttcccc ccacagcaca tcgacgagct ctccacgctc atacaactgc cagccacaga
     3721 aatcacagcc ctgttcggca taccagtgct ccagcgtgca gctgaaacgg cggcttaatt
     3781 ctgcaataac gtcgctttcc ggctgacacc acggcgtgtc gaaatccacc tgaatgaagt
     3841 tttctccctg actgctgata ttcaggtcat atccacaagg ccacttcacg ccataccgtt
     3901 ccgtatacca gtgatatgca gaaggaacac cgttcagaac accaccatta aagccgttca
     3961 cttcaacatc caggcgggtc ggcagaaccg tcagcaggtc aaacggcatg gttttttccg
     4021 gcactacgtt gtcacacaga cggttccacc acacagatac atcctcgtta ccccatatgt
     4081 cacaccagtc acctcttttt gcggtgaaat gcacagtgat ggtttccctg gcatcatccg
     4141 gtaatccttc ccagcgacgc tggccagtac cgctctgtaa ccagagttca tgcagcatca
     4201 ggcagttttg ttcatccagc aacacaccgt tctgcaggtg tgtcagccag cgggtgaacg
     4261 cgatattttc cgtcgataca gcaccacgtc cggcagccgt cactccgggg cactgttcag
     4321 agcggatatt ttcagtgatc tgcagaagtc cggcacttcc ggccagaaac agctgaatac
     4381 cttcatttgt ggcgcggcga taaagcggtg tgactgcacc gctggccagt cgtttaatct
     4441 cagcgatctg tgccggttca ccaggaaaat acatacgatt cgagcaccag tttggcatgg
     4501 gaattctcct ctcacatgcg gcctccatca ggccgtgccg cacctgtaac cgggcgaccg
     4561 catacgaatg caggtgcggc cccgaagccg cagcgcgtgg aggaggaatg acgaaacgtg
     4621 accgggtgag gaggccggcg acagcgacac acttgcatcg gatgcagccc ggttaacgtg
     4681 ccggcacggc ctggttaagt agtatccgtc tcagttcgtg gcgcaaaatg agaaaccaat
     4741 aagaactcaa aatgaaccac aacaagaaca taaagagact ctaaaagagc aacaccgctt
     4801 gacatagact caaaatgagt cacaataaga aacaataaaa aactcaaaaa gatgcaccga
     4861 tataaaggat gctcaatttg agataaacac aaactcaaaa cgaggcaaaa agaatgctaa
     4921 aagtatcatg cgatgatggt tcaactaacg taaaactggc ctggcttgaa gatggcgagg
     4981 taagaaccag tctgtcagga aacagcttta aagaaggctg gaacccggga ctgtttaatg
     5041 ccggcaaagt ttacaattac gtcgtggatg aaaaaaaata cacctatgat ttgggaagca
     5101 ctgctgtcat aggtacaaca catgtcagtt atcaatacag caccaccaat ctgcttgcca
     5161 tacatcatgc actgctgaca tccggccttc agccacagga tgtagaactg acagtaacat
     5221 tacctgtgac cgaattcttt gataatgaca atcaaccaaa tgaagaaaga atagaaagaa
     5281 aaaaggcaaa tgttctccgt gagatatctc tgaataaagg ggaaacattt aaaattaaaa
     5341 aagttaatgt aatgcctgaa tctttgccag ctgcatttga atcactaaaa aaagataaag
     5401 taaacaaact tgaacgcagc ctgattatcg atcttggagg aaccactctg gattgtggtt
     5461 taattctcgg tgcatttgaa ggcatttctg aaatccgtgg ctattcagaa atcgggacat
     5521 cccgcatcac tcataccgtc atgaatgcgc tgacgaaagc ctccacacca tgtaattatt
     5581 ttattgctga tgagttaata aaaaacagac acgataatga atatctccag acattaataa
     5641 atgatgtagc tgaaataaaa aacatttctc atgtaataga cagagaagtg aaatctctgg
     5701 cagaaagcat caggcaggaa atttcaacat tctcaggaat gaaccgaatc tatctcactg
     5761 gcggaggagc cgagcttatt taccctcaca taaagcaata tttcccgaat ctgaaagtga
     5821 acaaggtgga tgaacctcag tttgcattgg tgaaagcaat ggtgcatgcg taatggaaag
     5881 cagtgatcca aaaaaaagaa agaaagttgt ggcatacctg cacccggcac tttatccgca
     5941 ggacaacctg acacaacaaa caatagactc tctcccagta caaatgcggg gagatttcta
     6001 cagacaatcc cttatttgcg gagcagctct ttattctgtt gacccccgac tgttaacgct
     6061 aatcagcggt tttttcagtg agaaaatcac tgcagaaaat ctggtgaaac ttatagaaca
     6121 aacaacagga tacacatcca catcaattga tataagtgtg ttaaaaaaca tcatagaggc
     6181 atcttcagaa aacaaatcag agagcatcac ttcaaaagat gatttcgaag aacaaaccag
     6241 acgcaatctt tctatgttaa aaaaataata aaagaagagc aggttcagtc aattacctgc
     6301 tcttctttta tacatactaa aaaatcagat ttttacgaat attcagatta tcataaaaaa
     6361 tgcaacagac aggaatgcaa taattttgca ttatctcgtt gcttgtaatg caaatatgca
     6421 ttattgtcag tgtgtgacaa taacagagag atgacgatgg cgaccattaa cgcacgtatt
     6481 gatgatgata taaataacca agcagatgaa gtgctgaagc tgatgaatat cagtcaaact
     6541 caggctattg cagcttttta tcagtatatt actgaacaaa agaaactgcc atttgtgatc
     6601 acctcaattg taaaaacgcc gcatgattta ttacgtgaaa gtacagacat gttagctgaa
     6661 gctcttgcag tgatttccaa tctacaggtg tggactgaac agcaggatgg gattggaaaa
     6721 gcaaagctaa tggagtatta ccgccgcctc gacgcactgt actgttgtgc aaaagagaaa
     6781 ataggtcttc tttcagataa ccgggatgct gaa
//
LOCUS       DADMGC010000175         6802 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.175, whole genome shotgun sequence.
ACCESSION   DADMGC010000175 DADMGC010000000
VERSION     DADMGC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.175"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            94..1518
                     /gene="sbcB"
                     /locus_tag="JNZ73_003771"
     CDS             94..1518
                     /gene="sbcB"
                     /locus_tag="JNZ73_003771"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HAY8733881.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWQEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(1561..1788)
                     /locus_tag="JNZ73_003772"
     CDS             complement(1561..1788)
                     /locus_tag="JNZ73_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HAY8733882.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1802..2860)
                     /gene="yeeE"
                     /locus_tag="JNZ73_003773"
     CDS             complement(1802..2860)
                     /gene="yeeE"
                     /locus_tag="JNZ73_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HAY8733883.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALILAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3039..4397)
                     /gene="plaP"
                     /locus_tag="JNZ73_003774"
     CDS             complement(3039..4397)
                     /gene="plaP"
                     /locus_tag="JNZ73_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HAY8733884.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4387..4467)
                     /gene="yoeI"
                     /locus_tag="JNZ73_003775"
     CDS             complement(4387..4467)
                     /gene="yoeI"
                     /locus_tag="JNZ73_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HAY8733885.1"
                     /translation="MRNPKKWGKFFAYATVITVKENNHVA"
     gene            complement(4665..5594)
                     /locus_tag="JNZ73_003776"
     CDS             complement(4665..5594)
                     /locus_tag="JNZ73_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8733886.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(5640..6464)
                     /locus_tag="JNZ73_003777"
     CDS             complement(5640..6464)
                     /locus_tag="JNZ73_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8733887.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6547..6801)
                     /gene="yoeB"
                     /locus_tag="JNZ73_003778"
     CDS             complement(6547..6801)
                     /gene="yoeB"
                     /locus_tag="JNZ73_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HAY8733888.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1839 a         1781 c         1595 g         1587 t
ORIGIN      
        1 tatttgacgt tcgtagacat actcaggtga aaaaagggtt gcttttcgag ttataacaga
       61 cagttaatta actacagtgg acaatgctag acaatgacag acacagataa acaacctacc
      121 ttcctctttc acgattacga aacctttggc acgcaccccg cgttagatcg ccctgcacag
      181 ttcgcagcca ttcgcaccga tgacgaattc aatgtcatcg gcgaacccga agtcttttac
      241 tgcaagcccg cggatgacta tttaccccgg cctggagcag tattaattac cggtattacc
      301 ccgcaggagg cacgggcgaa aggagaaaac gaagccgcgt ttgccgcccg tattcactcg
      361 ctttttaccg taccgaagac ctgtattctg ggctacaaca atgtgcgttt cgacgacgaa
      421 gtcacacgca acgtttttta tcgtaatttc tacgatcctt acgcctggag ctggcagcat
      481 gataactcgc gctgggattt actggatgtt atgcgtgcct gttatgccct gcgcccggaa
      541 ggaataaact ggcctgaaaa tgatgacggt ctaccgagct ttcgccttga gcatttaacc
      601 aaagcgaatg gtattgaaca tagcaacgcc cacgatgcga tggctgatgt gtacgccact
      661 attgcgatgg cgaaactggt aaaaacgcgt cagccacgcc tgtttgatta tctctttacc
      721 catcgtaata aacacaaact gatggcgttg attgatgttc cgcagatgaa acccctggct
      781 cacgtttccg gaatgtttgg ggcatggcgt ggcaatacca gctgggtggc accgctggcg
      841 tggcatcctg aaaatcgcaa tgccgtaatt atggcggatt tggcaggaga catttcgcca
      901 ttactggagc tggatatcga tacattgcgc gagcgtttat ataccgcaaa agccgatctt
      961 ggcgataacg ccgccgttcc ggttaagctg gtgcatatca ataaatgtcc ggtactggcc
     1021 caggcgaata cgctacgccc ggaagatgcc gaccgactgg gaattaatcg tcagcattgc
     1081 ctcgataacc taaaaattct gcgtgaaaat ccgcaagtgc gtgaaaaagt ggtagcgata
     1141 ttcgcggaag ccgaaccgtt tacaccttca gataatgtgg atgcacagct ttataatggc
     1201 tttttcagtg acgccgatcg tgcagcaatg aaaattgtgc tggaaaccga gccgcgtaat
     1261 ttaccggcac tggatatcac ttttgttgat aaacgaattg aaaagttgtt gttcaattat
     1321 cgggcacgca acttcccggg gacgctggat tatgccgagc agcaacgctg gcaggagcac
     1381 cgccaccagg tcttcacgcc agagtttttg cagggttatg ctgatgaatt gcagatgctg
     1441 gtacaacaat atgccgatga caaagagaaa gtggcgctgt taaaagcact ttggcagtac
     1501 gcggaagaga tcgtctaatt tgaagccgcc gccgctggta cggcggagga caatgttgag
     1561 tcaggctttt tgaacggtga tgctccacgc tgcatcgcca atctgctgat aatcggtgat
     1621 ggcatgtcct tcttctgccg cccactgtgg gattgcttca gttgcctggg tacaatcaaa
     1681 ctcaattacc aactcatcgc cgctggccat ttctgccagt gctgcttttg cttctatgag
     1741 tggaaacgga cagacctgcg tcaccacatc cagcttttta atcgccatct ttacacctta
     1801 cttaatttgc cgcagcagtt gccagtcgcg ccttacgctg cggtcgaaca tacacaagcc
     1861 aggacgcagt ccagactccg agaatcataa atacaaggcc aatccagccc tgccaggtca
     1921 tcattgcagt cataaccagt ccattaccga tagagcaacc acctgcaata ctggcaccga
     1981 agcccatcag tacaccacct aacccgctac gtaatgttgt ttgtgcatca gctgcgcgaa
     2041 cgcggaactc acggctcgct ttggctgcaa taaatgcccc cacgaagatc cctaacacca
     2101 ggaaaacgcc ccagttaatg tatttcacgt cacccgcgac cagaaattgc agaatattgg
     2161 ccgttgggga agtgactcct aacccaaaca tgcgtccggt tgcttcactc aggggccagg
     2221 ctaaaagcgc aatcaaaccg atgagtacag cagtgacaaa gggatgccag cgtttttcaa
     2281 acagaatatg agcgatcccg gttcggcgcg gcggtaaggt cgcgactttg agttttggct
     2341 tcttcaactc tttcatcacc acccagagcg ttatcaccag caaaacggta accaacggcc
     2401 acacagacaa attaaaagtc tcagcaatag agttatgttc agtagtgtag tgctgcaagg
     2461 tttgatttaa accactggcg tgtggagaac gcatcaccgc actcatcacc atataagtga
     2521 aaagcgcgat ccaactgccg atcaatccct cacccgcgcg ataccaggtc ccggtggcac
     2581 atccacccgc cagaacaatt cccagcccga aaagataccc acctataaca gtaccaagcc
     2641 acgggaacgc cccggcttcg taagtcagta ggcccgccag aattaacgca aaaaccccca
     2701 cgctttgtgc cgaaattgca atcagcaagg cgtaaaacat gcgattattt ttcgcgatat
     2761 acatatcgcg aaaaccacca gtcaggcaaa aacgcccacg ctgcatgaca aatcccagca
     2821 gagcaccaca aattagcccg cttaatatca ttgaaaacat aaattaatta accagatgaa
     2881 tgttaatggg gaaatttttc atgactggtg gaatgcagac caataaccaa attctctaaa
     2941 ttagaacaaa tggttattaa tgaggatttc gataaaaaag aataaaaaaa ccggagccaa
     3001 atgttcattc gactccggtt tattattaga aaagatgatt atgctacgtc ttcgtactgc
     3061 ggaaccggat tacggaagct cttggtgacg caagccaggt aaatcagacc gatagcagcc
     3121 cagatcagac ccagaaccat tgagctttct tccaggttaa cccacagcgc accaacggtc
     3181 agcgcaccac acatcggcag gaacagatac tggaagtgat ctttcagcgt cttgttacgc
     3241 ttctcacgga tccagaactg cgagatgacc gacaggttaa caaaggtgaa cgccaccagc
     3301 gcaccaaagt taatcagcgc cgttgccatt accaggtcga agttgattgc cagcagggca
     3361 atcgcgccaa ccaggatgat gttcatcgcc ggagtacgcc atttcgggtg tacataaccg
     3421 aagaagcttt tcgggaacac gccgtcacga cccattacgt acatcagacg cgctacgcct
     3481 gcgtgcgccg ccataccgga cgccagtacg gtaatggtgg agaagatcag cgcgccaacc
     3541 tggaacgctt tacccgcaac gtacagcatg atttcaggct gtgacgcatc cggatcttta
     3601 aagcgagaga tatccgggaa gtacagctgc aggaagtaag ttgcaaagat gaagatcatg
     3661 ccgccaatca gcgcggtcag gaaaatcgca cgcgggatca cgcgctctgc atctttggtt
     3721 tcttccgaca gattgctgat gccgtcaaag ccggtaaagg agaagcacag gattgtcgcc
     3781 ccggtaatca tcgggataac atgcgcatcg ccagaccaga acggacgcgt gctcgccagc
     3841 gtaccagcgc cttcaccttc aaatacgcca taaacaacca tgcccagaat caccgcgatc
     3901 agcactacct gcaacacgac gattacagtg ttgaagttcg ctacggattt cagactacgc
     3961 aggttaaagg cggtcataaa ggccaccagc gccaccacaa acatccacga tggaatggaa
     4021 ggcaccagag cttcaaaata gattttcgcc agcagaatgt tgatcatcgg cgcgaacaga
     4081 tagtcgagca gagaagacca acccaccata aagccgacag tcgggctaat ggatttctgg
     4141 gcgtaagtgt atgcagagcc agcagaagga tagcggcgaa ccagcttccc gtagctcaga
     4201 gccgtaaaca ggatcgcaat caatgcgaac gcataggctg tcggcacatg accatccgtg
     4261 aggcctgaaa cgataccaaa tgtatcaaac agcgtcatcg gctgcatata ggctagaccc
     4321 atcattacaa ccggaactaa cgtaagcgtt ttacgcaatt ccacgcgaga ggtgtttgga
     4381 gtaacgttat gcgacatggt tattctcctt tacggtgata accgtcgcgt aagcaaaaaa
     4441 tttgccccat ttttttggat tcctcagcga caacaactgt cgatttttag taaatatcta
     4501 tccggtacga agcccggcct cttggtatga atgattggtt tgaagcaaaa aataaccgac
     4561 gctaattaaa cgtcggtttt tagtcatttt ttgacagcgg cgcattgtgc ctaaatgggg
     4621 gggaaatgac aagagaatga gaggcttgtc ataataattt ttctttaaat ggctgatttt
     4681 ccgtcatcag attttgcata aacaccggcg atcgcactat ttgctaaaat ttcatctcgc
     4741 caccagccgc tggcaagccc tgcggtctgt tcattccagc caatccagac aggttcaaag
     4801 agagtttggg cgaccacttt cttctcgatt aacgcgccac tatcgagaaa acgttgcgcc
     4861 agataccgtg gtaaatagcc gcagccaagg ccgctaattt gcagctccag tttggtttta
     4921 aaatcgaaaa cggtaatggc ttcttgttcg tcgagaagtt gtgaggccgt agaagcgcct
     4981 gcctgcgcag tgtcccccac cacaatcgcc cgatatcgtt taatgatacg acggtttaac
     5041 ggttcttctt cctgggctaa cggatgatga ggcgcgaccg caaagacttg ttcaagatcg
     5101 cccaaccgcg agaaaccaaa ctcactggaa gatggtggct catgcatcgc gccaacgata
     5161 atatccgctc gcccctgagt cagggcatcc caggaaccac cgagtacgcc attgataaac
     5221 ttcaggcgcg tcacgctatg atgttgataa aaagcttcaa tgagaggcgc aagcagagaa
     5281 aaaggaaacg tgtcgtccac gccaatcacc agctcgtttt cccagccttc atgcagtttg
     5341 atcgcctgct tttccagttc tcgcacggta tgcagaactt cccgcccttt ctctaataac
     5401 atttttccgg tgcgggtgaa tttagcgcgg tgtccgctac gatcaagcaa ttgaatattt
     5461 aggtcgcttt ccagcttgtg aacggtgtaa ctcagagcgg acggcgtctt gtagagtttg
     5521 gctgacgccg ccgcaaaact gccctctttt tctatggcat cgaggatcat caacacatcc
     5581 agcagtggtt tcatacacgc ccccttgcag tgcatgcggc gtcctgctgg cgtgatcgtt
     5641 tactccagcg gcattaccag cggatcggga tactggtatt caaatcccag ttcattacaa
     5701 atccgactgc catcaataat cttacctttg ccgctgtcca gactatttct gaactgcgga
     5761 ggttgcagcc ccagtaaacg ggccatctgc ggatagaaaa cattacgcgc agggtgagct
     5821 ggcgcacata tattatagat gtgtccgcct ttaggtgcct gtaacaacag agtgatagcg
     5881 ccaataacat cttctaaatg gactaaatta acaccatgtt caccatcagg ggcggttttt
     5941 ccggcaaaga agcgtcccgg atgacgtccc ggtccgacca ggcccgcaag acgcagaata
     6001 tcgaccgaag taccgggtaa attgtgcagc cagtcttcga gttcttctaa tactcgtcca
     6061 ctgttggtta ccggattacg cggggtagtt tctttaaccg tgccttgcgc gtcgccatag
     6121 acagatgttg agctggtaaa aataattcgg ggaatacgat gggccagcgc gctatccact
     6181 aactcttgta ccgcttgtaa atagaactca tcgccggggc cgctacgacg tgccggaagc
     6241 gtaatgacca gcgcatcggc atccataagg gcatccagat cgtcagaatc gcaaactaac
     6301 tcaggctcca tgcgaagcag atagctatca atgccactca ttcgggccgc ttcgacacca
     6361 tcttgtgtgg ttttactccc ggtgacttgc cagcctcgcg ctgaaagtga catcgccagc
     6421 ggcataccta accaccctaa cccgacaatt gcgacctttt tcatccgtta tctcctgata
     6481 caccttagat ctataaggct acgctagcgt atcaaaactg acaattcatt ctatgaatga
     6541 atctgttcaa taatgataac gacatgctgc aatgagcagt gaatcatcag taaccgcgta
     6601 taccagacgg tgctcctctg taatgcgtcg ggaccagaaa cctgacaaat tatgtttcag
     6661 gggttctggt ttccccttac cttcaaatgg tgttctgcgg gtatctttga taagttcatt
     6721 gatcttttta acaattcgct tatctgtttc ctgccagtac agataatcat cccatgattc
     6781 ctcagaccag attagtttca ct
//
LOCUS       DADMGC010000176         6793 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.176, whole genome shotgun sequence.
ACCESSION   DADMGC010000176 DADMGC010000000
VERSION     DADMGC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.176"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            239..2671
                     /locus_tag="JNZ73_003779"
     CDS             239..2671
                     /locus_tag="JNZ73_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAY8733889.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEALI
                     HELEQQGIIWEVLAEEVGKDLDPFDILCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2702..4198
                     /locus_tag="JNZ73_003780"
     CDS             2702..4198
                     /locus_tag="JNZ73_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HAY8733890.1"
                     /translation="MLQKALFFMEYNMSISSVIKSLQDIMRKDAGVDGDAQRLGQLSW
                     LLFLKIFDAQEEALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPAL
                     KNLTAPIDKNPRGYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIY
                     EQILKDLQSAGNAGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKN
                     KYVKSVADHQTLQQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWD
                     EQLDVIVTNPPFGGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDG
                     TLFGEGVKTKIKKLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYE
                     HPYPAGVKNYSKTKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFN
                     LDIKNPHQAETVSHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGK"
     gene            4207..5868
                     /locus_tag="JNZ73_003781"
     CDS             4207..5868
                     /locus_tag="JNZ73_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAY8733891.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6089..6430
                     /gene="symE"
                     /locus_tag="JNZ73_003782"
     CDS             6089..6430
                     /gene="symE"
                     /locus_tag="JNZ73_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HAY8733892.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6477..>6793)
                     /locus_tag="JNZ73_003783"
     CDS             complement(6477..>6793)
                     /locus_tag="JNZ73_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733893.1"
                     /translation="FDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRL
                     SLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESA
                     LV"
BASE COUNT         2004 a         1613 c         1549 g         1627 t
ORIGIN      
        1 ttcttgttcg aaagaaacgc aactataaga tttttatata ttttgttaga gttgatatgt
       61 ttcctgtaac ttctgtttat ctgtctctga tatgcccaga ttaattcaag caagatataa
      121 cagagatatc aatgtgatct ctgctacgta ctttacgttg aaacaccgcc ctaaatcata
      181 gccaggctat tgattttatg tacaatcaga atcgtcctga taaaagcgag aacaccccat
      241 ggcggaactg aacctaagta acctgacgga agcagacatc attaccaaat gcgttatgcc
      301 agccattctc aatgcaggct gggacaacac aacgcaaatc agacaggagg tcaaactccg
      361 ggacggtaaa gtcatcgtac gtggtaaagt tgcggcacgc agaacggtaa aatctgctga
      421 catcgtgctg taccacaagc caggcattcc cttagccgtg attgaagcaa aagccaacaa
      481 acatgaaatt ggcaaaggga tgcaacaggg cattgaatat gcgcgcctgc tggacgttcc
      541 ttttgttttt gccaccaacg gtgatggctt tatcttccgc gatgccaccg cagccgaagg
      601 tgaatgcctt gaaaagcaaa taacgctgga tgacttcccc tcccccgctg aactctggca
      661 aaaattctgc ctctggaaag gatacacaca agctcagctt ccggtgatta ctcaggacta
      721 ttacgacgat ggcagcggca aatcgcctcg ttattatcaa cttcaggcaa tcaacaaaac
      781 cattgaagcc gtctccaacg ggcaaaaccg cgttctgctg gtcatggcga ccggaacagg
      841 gaaaacctat accgcattcc agatcatctg gcgcctgtgg aaatcaaaaa ataaaaaacg
      901 cattttgttc cttgccgatc gcaacattct ggtcgaccaa accaaaaata atgatttcca
      961 gccatttggt acggcaatga ccaaagtcag cggacgcacc attgatcccg cttatgaaat
     1021 tcacctcgcg ctctatcagg ctataactgg cccggaggaa gaccaaaaag cgtttaaaca
     1081 agtcgcacca gatttcttcg atctgatcgt gatcgacgaa tgccatcgcg gcagcgcatc
     1141 cgaagacagc gcctggcgag agatccttga ttatttcagt tccgccaccc aaattggctt
     1201 aaccgccacg ccaaaagaga cgcatgaagt ctccagcacg gattacttcg gcgatccggt
     1261 ttacgtctac tcactaaaag aagggatcga agacggcttc ctcgctcctt ataaagttgt
     1321 ccgtgttgat attgatgtcg acctgcaagg ctggcgccca accaaagggc aaactgactt
     1381 aaacggcgaa gtgattgacg atcgtatcta taaccagaaa gatttcgatc gcacgatggt
     1441 gatcgacgaa cgcacagaac tggttgccag aaccattacc gactacctca agcgcaccaa
     1501 tccgatggat aaaaccatcg tcttctgtaa cgacatcgat catgcagaac gtatgcgccg
     1561 cgccctggtt aatctcaacc cgcagcaggt gaaaaagaac gacaaatacg tcatgaaaat
     1621 caccggcgat gatgaaattg gcaaagctca gttggataac ttcatcaacc cgaaaaaacc
     1681 gtacccggtt atcgcgacca cttcagagct gatgaccact ggtgtggatg ctaaaacctg
     1741 caaactggta gtactagacc agaacatcca gtcgatgacc aaattcaagc aaattatcgg
     1801 tcgtggtaca cgcatcgacg aacgttacgg caaactctgg tttaccatcc tcgactttaa
     1861 aaaagccacc gaactgtttg ccgatgagcg tttcgatggc attcccgaaa aagtcatgga
     1921 taccacacca gaggatatcg ccgatccaga atctgatttt gaagagaaac tcgaagaaat
     1981 cagcgaacat gacgacgaac aggtaacagg cgttgatgaa ccgcctgcgc caccatacca
     2041 ggttaccgat accgatgatg tcggcccatt tccggaagaa gacgagaaga aaatccgcaa
     2101 gtttcacgtc aacggtgtag cggtgggcgt tattgcacag cgcgttcagt attacgacgc
     2161 cgacggtaaa ctggttaccg aatcctttaa agattacacc cgcaaaacac tgctcaaaga
     2221 atatgcctcg ctggatgact ttacccgcaa gtggcaggac gccgatcgca aagaagcgct
     2281 cattcacgag ctggagcaac aggggatcat ctgggaagta ctggcagaag aagtcggtaa
     2341 agatctcgac ccgttcgaca ttctttgcca cgtagtgtat ggtcagccgc cgttaacccg
     2401 caaagagcgc gccgagaacg tgcacaagcg gaactacttc acaaaatact ctgaagcagc
     2461 gcaagccgtg ctcgataatc tgctggataa atacgccgat gcgggcgtac aggagatcga
     2521 aagtattcag gtgctgaaac ttaaaccatt cgacagcatg ggcaccttac cggagattat
     2581 taaaaccgga tttggcgacc gtaacgggta taatcaggcg ctcagcgagc tggaaaacga
     2641 aatctaccaa ttaccgcccc gctctgctta acaactctct tttgcgcctt ctgcaaccat
     2701 catgttgcag aaggccttgt ttttcatgga atacaacatg tctatcagct cagtaatcaa
     2761 atcattacaa gatattatgc gcaaagatgc cggtgtggac ggcgatgcgc agcgtctcgg
     2821 tcagctctcc tggctgctgt ttttgaaaat cttcgatgcc caggaagagg cgctggaact
     2881 ggagcaggat aactatcaat atccgatccc acagcgttat ttgtggcgca gttgggccgc
     2941 caacgcgcag ggcattaccg gtgattccct gctggaattc gttaatgatg atctgtttcc
     3001 ggcgttgaaa aacctcactg cgcctatcga taaaaaccca cgcggctacg tggtgaagca
     3061 ggcgttcagc gatgcctata actatatgaa aaacggtacg ctactgcgcc aggtgatcaa
     3121 caagctgaac gaaattgact ttaccagcgc cagcgaacgc catctgtttg gtgatattta
     3181 cgaacagatc cttaaagatc tgcaatctgc gggcaatgcg ggcgaattct atactccacg
     3241 cgccgtcact cgctttatgg tggatcgcgt tgatccgaaa ctcggcgaat ccattatgga
     3301 cccggcctgc ggtacgggcg gttttcttgc ctgcgcgttc gatcatgtaa agaacaaata
     3361 cgtgaagagc gtcgccgatc atcagacact gcaacaacag atccacggtg ttgagaaaaa
     3421 acagcttccg cacctgctgg cgaccaccaa tatgctgctg cacggcattg aagtaccggt
     3481 acaaattcgt cacgacaaca ccctgaataa accgctttcc tcctgggatg agcaactgga
     3541 tgtcattgtt accaacccgc cgtttggtgg cacggaagaa gacggtattg agaagaactt
     3601 tccggcagag atgcaaaccc gcgaaacggc ggatctgttc ctgcaactga ttgtggaagt
     3661 actggcgaaa aacggtcgtg cggcggtggt attgccggat ggcacactat ttggcgaagg
     3721 tgttaaaacc aaaatcaaaa agctacttac cgaagagtgc aacctgcata ccatcgtgcg
     3781 tttaccgaat ggtgtgttta atccctatac cggcattaaa accaacctgt tgttctttac
     3841 caaaggtcag ccaaccaaag agatttggtt ctatgaacat ccgtatccgg cgggcgtgaa
     3901 aaactacagc aaaaccaagc cgatgaagtt tgaagagttt caggcggaga tcgactggtg
     3961 gggtaacgag gccgatggtt ttgccagccg cgtcgagaat gagcaagcgt ggaaagtcag
     4021 cattgatgac gtgattgcac gcaacttcaa tctggatatt aaaaacccac atcaggcgga
     4081 aaccgtcagc catgatccgg acgaactgtt agcgcagtat gcaaaacagc aggcggagat
     4141 ccagacgctg cgtaatcaac tgcgcgatat tcttggcgct gcgctgtctg gcaagtaggt
     4201 taactaatga gtgtggaaaa gctgatcgtt gatcatatgg aaacctggac ctcggcgttg
     4261 caaacccgtt ccaccgccgg gcgcggcagc tccggtaaga ttgatttgta tggtattaag
     4321 aaattgcgtg agctgattct ggaactggcg gtgcgcggta aactggtgcc gcaggatccg
     4381 aatgatgaac cggcgtcgga cctgctgaag cgtattgcgg cggaaaaagc agagctggtg
     4441 aagcaaggca aaattaaaaa gcaaaaacca ctgccagaaa ttagtgagga agagaagccg
     4501 tttgaattgc cggagggatg ggagtgggtg cacctccctg atatttattg ctcaatttct
     4561 gagtcatcta gaaaaataaa atcctccgaa atactacctg agggaaaata tcctgttatc
     4621 gagcaaagcc aagaatttat ttctggttat tgcaacaatg aatgtctgtt aataaagtta
     4681 aataatccag ttatagtttt tggtgatcac acaaggaata ttaaatttat tgattttgat
     4741 tttgttgtcg gcgctgacgg tgtgaaaatt ttatcaccta tattaatttg tgagcgtttc
     4801 tttttttggc agttacgatc atttaaatta gatgtaagag gatacgcgag acattttaaa
     4861 gtattaaata gctgcttatt tgcattaccc cccattgcag aacaagagcg aatagttgaa
     4921 aaagttagct ctttgatgtc cctctgcgac caactagaac agcaatccct gaccagtctg
     4981 gacgcacatc agcaactggt tgaaaccctg ttgggaacgc ttacagacag ccaaaacacc
     5041 gcggaactgg ctgaaaactg ggcgcgtatt agcgagcatt tcgacaccct atttaccacc
     5101 gaagccagcg tggatgcgtt aaaacagacc attctgcaac tggccgtaat gggcaaactt
     5161 gtgccgcagg atccgaatga cgaacccgcc tctgaactgc tcaaacgtat tgcgcaggaa
     5221 aaagctcaat tggtgaaaga agggaaaata aaaaaacaaa aaccactgcc gccaattagc
     5281 gatgaggaaa aaccgtttga acttccggaa gggtgggaat ggtgtcgtat tggtaatata
     5341 gttaatatta aatctgaatt agtatcacca aaagattatt taaatttata tcaagtagca
     5401 cctgatatta ttgaaaaggg cactgggaga gtgatatcaa agagaacagt gaaagaatca
     5461 ggagtcaaag ggccaaatag tcgcttttat aaaggacaaa ttgtttattc taaaatccgc
     5521 ccgtcattat ccaaagtttt tttagcagag tataatggtc tttgcagtgc tgatatgtat
     5581 ccattagact gttacattaa tccaaattac ttattgaaat atatcctttc cattccattt
     5641 cttatgcaag tcaaaaaagc agagaacaga ataaaaatgc cgaaacttaa ttctgacagt
     5701 ttttacaata ttattgttgc gattcccccc tataatgaac aacaagcaat atttgataag
     5761 ataaattcta ttgaagcagt atgcaatggt cttatttctt atattgggat ttatcataaa
     5821 acccaactgc atcttgcaga cgcactcact gacgcagcga taaactaatt ccctcgccct
     5881 cacaaaacca ataaagcggc taaatagccg ctttattcac atcaccaaaa tttatatttc
     5941 acgctaattt tcatcttatt ttcattaatt tttagctgga ctttccctgt atttactgat
     6001 gatatatcca ggtatttagc acggtgcgga tgtgcgccaa cacactcgca ccgctaatca
     6061 caatcactat ttatagagaa tagtaatcat gactgacacg cattctattg cacaaccgtt
     6121 agaagcagaa gtctccccgg caaataatcg tcaattaacc gtgagttatg cgagtcgtta
     6181 cccggattac agccgtattc ccgccatcac cctgaaaggt cagtggctgg aagccgccgg
     6241 ttttgccacc ggcacggcgg tagatgtcaa agtgatggaa ggctgtattg tcctcaccgc
     6301 ccaacccgcc gccgccgaag agagcgaact gatgcagtca ctacgcaagg tgtgtaagct
     6361 gtcggcgcgt aaacaaaggc aggtacagga atttattggg gtaattacag gtaaacagaa
     6421 agtcgcctga tacattgacc cggtagcgcc agtgctaccg ggtattattc gccgcgttat
     6481 accaacgcgc tctccatctg tttttcaaat gcctgacgaa ttaatcccat aatgtaagga
     6541 agttgtgcga ggtcgctgaa accaatttct acatcgccat taccccagcg cccaacattt
     6601 gtcacatctt tggcaatacc tttcggatcg accagttcat gaaactgcat attaagcgac
     6661 aatcgcagtc ggcttttttg cggcacaaca tcaacaaaat tcgtttccgc tttaaacgca
     6721 atatatagct tcaatacttc ctgcgtgatc cctgcgtcca ggcgcataac ttcatcgcga
     6781 agatgatcga ata
//
LOCUS       DADMGC010000177         6781 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.177, whole genome shotgun sequence.
ACCESSION   DADMGC010000177 DADMGC010000000
VERSION     DADMGC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.177"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            1..3524
                     /locus_tag="JNZ73_003784"
                     /pseudo
     CDS             1..3524
                     /locus_tag="JNZ73_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3534..6749
                     /locus_tag="JNZ73_003785"
                     /pseudo
     CDS             3534..6749
                     /locus_tag="JNZ73_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423056.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
BASE COUNT         1797 a         1780 c         1634 g         1570 t
ORIGIN      
        1 tgtggagcaa gcgaaagata attctttgca accttcccaa acggtaacgg actctctcaa
       61 ggtagcggac ttatccacca ttattcagga acaaccctct ttcgtagcag aaacccgtgc
      121 ggctgacaaa aatacagatg ctgttttacc ttggctggcg aaagatattg ctgtcgtttt
      181 tccgcccgaa gttgtacaca ccacgttaag tcatcgccgc tttcccggag ttcctgttca
      241 gcaagcagac aaattgaccc aattacgtcg cttagcctgt agcgtgtcgc aacgggataa
      301 taaaacagcc acatttgact tcagcacctg ttctttagaa tggcaaaaca ccgtcgccca
      361 agcgatcagt cagatcgacg gcctgaaaac aacacagcta ccatcaccag taatggctgt
      421 actcacggca cttgaaatga aatgcacaag atataaagtg cgtgaggatg ttatggatca
      481 gatcgtccag gaaggcggtc tggaatatgc tactgatgta ataatacacc ttcaacagat
      541 tgatattaaa tgggattatg cgaataatgt cattattatt ctgccgtctg gcattgcacc
      601 tgactacttg gagcaatatt ccagatttga attacgccta cgtaaacatt tatcactggc
      661 ggaagagtct ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc cacatattcc
      721 agaatggcgg caaccattaa ttgctttgtt attacccgaa aaaccagaaa ttgcacatga
      781 aattgcccag cgtctactgg ggcaaaaaaa attaccctcg cttgagtggt taaaaatagt
      841 ggcgactgat gagcacattc ttgcctcatt agaaaaatat cacgagccat atgccatttt
      901 tgatgattac tattgtggtg cgatatggtc agccaccgta ttacaggagc aaggtgttgc
      961 agccctgccc cgatttgctc cctatgccgc aagtgactac tgcgccgatg tgttgcgtca
     1021 tatcaatcat ccgttcgcat taacactgct tatacgtgta gccgggcata ctaaacgctg
     1081 tcacgatcga atgacgaaag cctgcgctgc gttcccacac gcagcactgg cagaacaggt
     1141 cattccctgg ctttcgacac ccgcagttgc agtgctgaaa tcatgccagc accaactgac
     1201 acagccctca aaccatgcca gcgccgatct actgccagcc atagtagtct ccccaccatg
     1261 gatttcgaaa aagaaaaaat cgccgattcc ggtgctggat ttagcgccat taggcatcga
     1321 gccaatctgt tatctgacag aagaaatcag taatcaactt ttggcgaaat atatctggta
     1381 ttcaaaacac atcacggtta gccatgaaga aagtactacc aacctgttgg caaggatggg
     1441 ttttcaacga cggatcgctg gtacatatat taaagctccc gaagcggtag ttgaggcatg
     1501 gctaaatgaa gattattcaa ccttactaag tgaatttaag gtgtttcatt cacctaccgg
     1561 gcattattgg cagttgggga ttttgacaac attgccgctg gaggctggag aaagcagtaa
     1621 aagcatggaa tgcccttact ctatctccac ataccgatac cgaatacgcc atgttacatt
     1681 ttggactcaa aggtttacct ggtttagtta actcaattac acgctatccg caggaagcct
     1741 tgcccatcac gaattacttc gcagcgagtg agctggctcc tgccgtcgcc cgtgccttca
     1801 acaaactcaa aacgctacgc gaaaacgccc gcacctggct gttgaaatac ccggaacatg
     1861 cccttaccgg cctgctgcct gcggcgctcg gcaaagccgg tgaagcacaa gataacgccc
     1921 gcgctgcctt gcgtatgctt accgaaaacg gtcatcagcc attactgcaa gaaatcgccc
     1981 ggcgttataa ccagtcggaa gtaacagacg cggtgaacgc tctgcttgcg ctcgatccct
     2041 tagataatca cccgacaaaa atccccactc ttccgacctt ttatcagcca tcgatctgga
     2101 cgcgcccggt attaaaagca aacgcccaat cactgccaga taacgccctc ctccacctcg
     2161 gcgaaatgct ccgcttccct caggaagagg ctctgtatcc gggattattg caggtgaaag
     2221 acgcctgtac cgctgactct ctggctgaat ttgcctggga tctgtttacc gcctggcaga
     2281 ccgctggcgc gccgtcgaaa gagagttggg cgttcactgc gttaggcgtt ctcggtaacg
     2341 atgacaccgc ccgcaaactg acgccattaa tacgcgcctg gcctggtgaa tcccagcata
     2401 aacgcgccac cgttgggttg gatattctcg ccgctatcgg tagtgatatc gccctgatgc
     2461 agcttaacgg cattgctcag aaactgaaat tcaaagcatt acaggagcgg gcaaaagaaa
     2521 agattgccga cattgccgag agccgcgaac tcacggtagc ggagcttgaa gatcggttag
     2581 caccggatct cggtctggat gataacggtt cgttgctgct ggattttggc ccacgccagt
     2641 tcaccgtcag ctttgatgaa accttaaaac cgtttgtgcg tgatgtttcc ggcagccgcc
     2701 tgaaagacct gccaaaacca aacaaaagcg atgatgagtc acaggcgaac gatgctgtta
     2761 accgctacaa attgctgaaa aaagatgcgc gtaccgtcgc cgcccagcag gtagcaaggc
     2821 ttgaatccgc catgtgcctg cgtcgccgct ggtcgccgga aaacttccaa ctcttcctgg
     2881 ttgtgcatcc gctggttcgc cacttaaccc gccgtctgat ttggggcgtt tatagcgccg
     2941 acaaccagct actggcctgt ttccgggtag cggaagataa cagctacagc accgctgacg
     3001 atgatctttt caccctgccg gaaggcgata tctctatcgg cattcctcac gttctggaaa
     3061 tatcaccgac ggatgctgtc gcctttggtc agctttttgc cgactacgaa ctgctaccac
     3121 cgttccgtca gcttgaccgt aacagttacg ccctgacaga agccgagcgc aacgccagcg
     3181 aactgacccg ctgggcaggc agaaaatgcc cgagcggccg ggtcatgggg ctggcaaata
     3241 aaggctggat aaagggcgaa ccacaggatg gaggctggat cggctggatg atcaaacctc
     3301 tcggtcgctg gtcgttaatc atggaaatcg acgaaggttt tgcggtgggt atgtcaccag
     3361 ccgaacttag cgctgagcag ctcttaagca agctgtggct atgggaaggc aaagcggaaa
     3421 gctatggctg ggggagtaat tcaacacagg aagcgcagtt ctccgtactc gatgccatca
     3481 ccgccagcga gctaattaac gatattgaag ccctgtttga ataaggaaag agcatggaca
     3541 aggaattacc gtggctggcg gataacgccc aactggaact gaaatataaa aaaggcaaaa
     3601 caccactcag tcatcgcaat tggccgggcg aaccagtgcc tgttatcact gaaagcatca
     3661 ttcagacact gggtgatgaa ttgctacaaa aagctgagaa gaaaaaaaac attgtctggc
     3721 gttatgaaaa tttttcaccg gagtggcagt ccgccattac gcaggccatc aacttgatcg
     3781 gcgaacacaa accctcagtc ccggcccgca caatggcggc actagccttt atcgcgcaaa
     3841 atgacaacca atagttgctc gacgaaatcg tccaacaaga ggggctggaa tatgcgactg
     3901 aggtggtgat tgcacgccag tttattgcac ggtgttatga gagtgatcct ctggtagtca
     3961 cattgcagta tcagaacgag gactatggct atggttatcg ctcagaaacc tataacgaat
     4021 tcgatctccg actgcgtaaa cacctctctc tggcagagga aagctgctgg cagcgttgcg
     4081 ccgacaaact cattgccgca ctgccaggaa taaccaaagt tcgccgccct tttattgcgc
     4141 tgatcctccc cgaaaaacca gaaatcgcca atgagttggt aagccttgaa tgcccgcgga
     4201 ctcattttca ttctaaggag tggttaaaag ttgttgctac tgaccccaaa gcggtgagaa
     4261 aactcgaacg ctactggagc caggatatat ttagcgatcg agaagccagc tacatgtcgc
     4321 atgaaaacca cttcggctac gcggcctgcg ctgccctttt gcgcgaacaa ggactggcag
     4381 tcgttccgcg cctcgcgata tatgcccata aagaagattg cggcagtctg ctggtacaaa
     4441 ttaaccatcc gcaagtcatc cgcaccttgc tgttagttgc tgataaaaac aaacccagcc
     4501 tgcaacgtgt ggctaaatac agtaaaaact tcccccatgc gacactcgcc gcactggcag
     4561 aactgctggc gttaaaagaa ccaccagccc gcccaagtta tccaatcatc gaagacaaaa
     4621 agctgcctgc acagcaaaaa gctcgagatg aatactggcg tacgctgtta caaacgctga
     4681 tggcatcgca gccacaactg gcagcagaag tgatgccgtg gttaagtact caagcccggg
     4741 cagtgctgaa tagttattta tcggcaccgc ccaaaccagt tattgatagc accgataaca
     4801 gccaaatgcc tgaaatcctc gtttcaccac catggcgtgg gagaaagaaa aagacttcca
     4861 tacgtcactt ttcgctgcct gatcttacgc ttgcaccgca aacgtacagg tcgccgaata
     4921 aacactggac gccggaacag caaactgcaa ttgaccattt tagcgccatg tcttttgacg
     4981 aacgtcttgc tacgagagga gcggatatct tcttgcggga attaggtttt gacgttcata
     5041 gcctggaatt taataagcgt cttcttaatg gacagttgga tgcctttgat gctcagcaac
     5101 tgcaagaata cacatcaata ggactattgc gtacttatca cgcgcatcag gccaaatcta
     5161 aacagtaccg ggaagacgcc accaatgcat tactggcaca agatagcatt gcgttgatgg
     5221 gagggtggcg aacttttcag cacggatttt actatggtaa tgaacagcgt tgggggattt
     5281 ggaatctgta ccttatcgcg cagatgccgc gcgaaatggc ggtatcgtgc tggcaacaaa
     5341 ttgtcgaggc tgattttcat tacaccgggg tggaatattt gttaagtgtg ctgggtaccg
     5401 atgctttgcc tggattaaac accgcctttg cccgacatcc aaaagagata tttccgctgc
     5461 tgattcattt cggcgcaaca gaactggccc tgcccattgc ccgcgtctgg cacctttttg
     5521 cgggccagcg taatctggct cgccagtgga ttttacaatg gccggaacat acggctactg
     5581 cacttatccc tcttgtcttc accaaatcta gcgataaaag cgaagctgca ttacttgccc
     5641 tgcgtttact ttacgaacat gggcatggcg aattgctaca aactgtagcc aatcgttggc
     5701 agcgtacaga tctatggcct gccctggaac aattacttaa acagagtcca atagagattt
     5761 acccggcacg cattccaaaa gcccctgatt tctggcatcc gcaaatgtgg tccaggccgc
     5821 gccttatcac taataatcaa cctgttaccg atgacgctct ggaaattatc ggcgaaatgc
     5881 tgcgctttac ccagggggga cgtttttata gcgggctgga acaactgaaa acgttctgcc
     5941 agccacaaac gctggcagct tttgcctggg atctcttcac tgcgtggcaa caagctggtg
     6001 cccccgcaaa agacaactgg gcatttctgg cgttaagtct ctttggtgac gaaagcacgg
     6061 cacgggatct aacgacacat atcctcgcct ggccacaaga aggcaaatct gcccgtgcgg
     6121 tcagtggcct gaacatcctt accctgatga ataatgatat ggcgctgatg cagctgcatc
     6181 atatatcgca acgggcgaaa tcccgcccct tacgtgataa cgcggcggaa tttcttcagg
     6241 tagtcgcaga aaatcgcggg ctaagccagg aagagttagc ggacagatta gtcccaacac
     6301 tgggccttaa tgatccgcag gcgttgagtt ttgattttgg tccccggcag tttaccgttc
     6361 gcttcgatga aaacctcaac ccggttatct ttgatcagca aaacgttcgc cagaaaagtg
     6421 ttccccggtt gcgcgccgat gacgatcaac tgaaagcgcc cgaggcactg gcccgactaa
     6481 aagggttaaa aaaagatgct actcaggtga gcaaaaacct gctcccgcgt cttgaagctg
     6541 ccctacgtac cacccgacgc tggtcgctgg cagattttca ttctctgttt gttaatcatc
     6601 cctttacccg tctggttacc cagcgattaa tatggggggt tatccggcaa atgaaccgcg
     6661 tcgtttactc aacgcctttc gtgtggccgc agagggggag ttctgcaatg cgcaagatga
     6721 gccaattgac ctgcctgcgg acgctctgat tggcattgcc cacccgttag aaatgacagc
     6781 a
//
LOCUS       DADMGC010000178         6717 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.178, whole genome shotgun sequence.
ACCESSION   DADMGC010000178 DADMGC010000000
VERSION     DADMGC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.178"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..768
                     /locus_tag="JNZ73_003786"
     CDS             <1..768
                     /locus_tag="JNZ73_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY8733894.1"
                     /translation="QNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVGGNMSL
                     TYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAPGAAGL
                     EIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPTRGAVV
                     KAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSGLPEKG
                     QVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            779..1852
                     /locus_tag="JNZ73_003787"
     CDS             779..1852
                     /locus_tag="JNZ73_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8733895.1"
                     /translation="MNKYIKQWCFSVFMLSLSSVALAAPKGICTPDNGVFHSSLDFSG
                     YLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            2100..3140
                     /gene="pstS"
                     /locus_tag="JNZ73_003788"
     CDS             2100..3140
                     /gene="pstS"
                     /locus_tag="JNZ73_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAY8733896.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLEKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            3227..4186
                     /gene="pstC"
                     /locus_tag="JNZ73_003789"
     CDS             3227..4186
                     /gene="pstC"
                     /locus_tag="JNZ73_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HAY8733897.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            4186..5076
                     /gene="pstA"
                     /locus_tag="JNZ73_003790"
     CDS             4186..5076
                     /gene="pstA"
                     /locus_tag="JNZ73_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAY8733898.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            5168..5941
                     /gene="pstB"
                     /locus_tag="JNZ73_003791"
     CDS             5168..5941
                     /gene="pstB"
                     /locus_tag="JNZ73_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HAY8733899.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPELRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            5956..6681
                     /gene="phoU"
                     /locus_tag="JNZ73_003792"
     CDS             5956..6681
                     /gene="phoU"
                     /locus_tag="JNZ73_003792"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAY8733900.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGSDELDKLLAGKDSDK"
BASE COUNT         1698 a         1573 c         1746 g         1700 t
ORIGIN      
        1 caaaacgata attccggtag ctcacatcaa ctcggactca gcggtacggc gctggaaaga
       61 aataaccttt catggaattt aatgcagtcc tataacagtc atgatgatga ggttggcggt
      121 aatatgtccc tgacctatga tggctcttat ggcacggtga acggcagcta taactacagc
      181 caaaattccc agaggctgaa ttatggtatc agagggggaa ttctggcaca cagcgaaggg
      241 gtaacgttaa gtcaggagtt aggtgaaact attgctcttg ttaaagcacc tggggccgcc
      301 gggttagaaa tagataatat gcgcggtgct gcgacggact ggcgcggcta tacggtcaag
      361 acacagctaa acccttatga tgaaaatcgg gtagcaatca gcgataacta tttctcgaag
      421 tcgaatatag aacttgataa taccgtcgtt acgatggttc ccacgcgtgg tgcagtggtt
      481 aaagcggagt ttgtgactca tgtgggttat cgcgttctct tcagagtgtt aaatgcaaat
      541 ggtaaaccgg taccttttgg agccattgct gcgatacaag atgcaagttt ggcagattca
      601 ggaattgtcg gtgaccgtgg cgaactttat ctttctggtc taccagaaaa aggacaggtt
      661 acgttatcct ggggagaaaa cgcctcaaca aaatgcatct tcaattattc actttcgaca
      721 ccagaaagtg agagcggatt aattgaacag ggtgtgacat gtcattaaaa ggatttttat
      781 gaacaagtat atcaaacagt ggtgcttttc tgtgtttatg ctctcgttaa gtagtgtagc
      841 acttgcagct cctaaaggta tctgtacccc tgataatggc gtatttcaca gttcgcttga
      901 tttctcggga tatcttatta ccgcgaacga aaataaggtt ggaacgacat ttaacaccac
      961 tgtaactaat ggatcctcat atcttggacg ctgtcattgt gatactggta acgtaggaga
     1021 attcccctat atctattata cgtcaaaaat aaaccaggca ttaacttatg ctggggttca
     1081 ctctaatatt aattattatg atcttaatcc gaaccttgat gttgggattg ctatagatat
     1141 tcttggggtt gggtatgtga atgcaccttt tgaatatcat gctaacaacc cgtctggtaa
     1201 tacaaagtac aattgcaacc gcattgaacc tttaagtata tccagtgggg ctaaagcgat
     1261 agtatatttt tatatcaaga agacatttgc aggaaaattg attattcctg aaacaaagat
     1321 agtgacattg tatggaacaa ttagccgtga caccccggtg gattactcac agccgatggc
     1381 tgatgtttat attcggggcg atattacagc tccgcaaagt tgcgaaataa ataatttaaa
     1441 gccagtttgt tttgatttta aagaaatacc agctgcagat ttttcatctg ttgttggaag
     1501 tgcggtaaca acacataaaa ttactaaaac agtcactatt gagtgtgaaa acctagggat
     1561 actgaatacc gatgatatca gtacgtcttt ttatgctacc gaacccaata cggacaattc
     1621 aatggtcgta acttcaaact ctaacgtggg gataaaaatt tacgacaaaa ataataagga
     1681 aatcaaagtg aacggtggtg agttgccaac agacatggat aaatcaacag tgtatggtga
     1741 aaaatcaggt agtgtaactt tttcagctgc tcctgcgagc cttactggag ctcgcccagc
     1801 cccggggcaa tttactgcaa cagcgacgat aactgttgag attgtgcgct aataggtctg
     1861 acaacgattg ttcatcacat cgttgtcatc tcacccattt ctttatcact tttaaatgca
     1921 tcatgacaaa aagatggcat gtgggatttt catcttcaca tttaaaatca gtaagttatt
     1981 tggtaattcc tttctgtcat aaaaatgtca tattccttat atataactgt cacctgtttg
     2041 tcctattttg cttctcgtag ccaacaaaca atgctttatg aatcctccca ggagacatta
     2101 tgaaagttat gcgtaccacc gtcgcaactg ttgtcgccgc gaccttatcg atgagtgctt
     2161 tctctgtgtt tgcagaagca agcctgacag gtgcaggtgc aaccttccct gcgccggtgt
     2221 atgccaaatg ggctgacact taccagaaag aaaccggtaa taaagttaac taccagggta
     2281 tcggttcttc cggtggcgta aaacagatta tcgctaatac cgttgatttt ggtgcctctg
     2341 acgcgccgct gtctgacgaa aaactggctc aggaaggtct gttccagttc ccgaccgtga
     2401 ttggcggcgt ggtgctggcg gttaacattc cagggctgaa gtctggcgaa ctggtgctgg
     2461 atggtaaaac cctcggcgac atctacctgg gcaaaatcaa gaagtgggat gatgaagcca
     2521 tcgccaaact gaatccgggt ctgaaactgc cttcacaaaa cattgctgta gtacgccgcg
     2581 cagatggctc cgggacttcc ttcgtcttca ccagctacct ggagaaagtg aacgaagagt
     2641 ggaaaaacaa cgttggtact ggctctaccg taaaatggcc gatcggtctg ggcggtaaag
     2701 gtaacgacgg tatcgccgcg ttcgttcagc gtctgccggg tgcaattggt tatgttgaat
     2761 atgcttacgc gaagcagaac aacctggcgt acaccaaact gatctccgct gatggtaaac
     2821 cggttagtcc gaccgaagaa aacttcgcta atgcagcaaa aggtgcagac tggagcaaaa
     2881 ccttcgctca ggatctgacc aaccagaaag gcgaagatgc atggcctatt acctctacca
     2941 cgttcattct gatccacaaa gatcagaaga aaccagaaca aggcacagag gtgctgaaat
     3001 tcttcgactg ggcgtacaaa accggggcta aacaggcgaa cgacctggat tacgctagcc
     3061 tgccggatag tgtagttgaa caggttcgcg ctgcgtggaa gaccaatatt aaagacagta
     3121 gcggtaagcc gctgtactaa taaaactcca ggccgggtac ggtgttttac gccgcatccg
     3181 gcattacaaa atgactttgt aaacgcgttt aactgaagag taacttatgg ctgcaaccaa
     3241 gcccgctttt aacccaccgg gtaaaaaggg cgacataatt ttcagcgtgc tggtaaaact
     3301 ggcggcgctg attgtgctat tgatgttggg tggcattatt gtctctctga tcatctcctc
     3361 ctggccgagc attcagaaat ttggtctggc tttcctatgg accaaagagt gggatgcacc
     3421 gaacgatatc tacggggcgc tggtgccgat ctacggtacg ttggtgactt cgtttatcgc
     3481 gctgctgatc gccgtcccgg tgagtttcgg tatcgccctg ttcctgactg agcttgcgcc
     3541 tggctggctg aaacgcccgc tgggtatcgc cattgagctg ctggcagcca ttccaagtat
     3601 cgtttacggc atgtggggcc tgtttatctt tgcgccgctg ttcgccgttt actttcagga
     3661 gccggtcggc aatatcatgt cgaatatccc gattgttggc gcgctgttct ctggccccgc
     3721 atttggtatc ggtatcctcg cggcaggcgt gatcctcgcc atcatgatta ttccgtacat
     3781 tgcggcggta atgcgtgatg tgttcgaaca aaccccggtg atgatgaaag agtcggccta
     3841 cggtattggc tgcaccacct gggaagttat ctggcgtatc gttcttccgt tcaccaaaaa
     3901 tggtgttatc ggcggcatca tgctggggct gggccgcgcg ctcggtgaaa ccatggcggt
     3961 gacctttatc atcggtaaca cctaccagct cgacagcgcc tcgctgtaca tgccgggcaa
     4021 tagtatcacc tctgcgctgg cgaacgaatt tgcggaagcg gaatccggtc tgcacgttgc
     4081 cgcactgatg gaactgggcc tgatcctgtt tgtgattacc ttcatcgtcc tcgccgcatc
     4141 gaagtttatg attatgcgcc tggctaagaa tgagggggca cgctaatggc tatggttgaa
     4201 atgcaaacca ctgcggcgct ggctgaatct cgccgcaaaa tgcaggcgcg tcgccgcctc
     4261 aaaaaccgta ttgcgctgac gctctcgatg gcgacgatgg ccttcggcct gttctggctg
     4321 atctggattt taatgtccac catcactcgc ggtatcgacg gtatgtcgct ggcgctgttc
     4381 actgaaatga cgccgccgcc caatacggaa ggtggtggtc tggcgaacgc cctggcgggt
     4441 agcgggctgt taattttgtg ggccacggta ttcggtacgc cgctgggcat tatggcgggg
     4501 atttatctgg cggaatatgg tcgaaaatcc tggctggcag aagtgattcg cttcattaac
     4561 gacattctgc tctccgcgcc gtcgattgtg gttggtctgt ttgtctacac catcgtggtg
     4621 gcacagatgg agcacttctc cggctgggcg ggcgtaattg cgctggcgct gttgcaggtg
     4681 ccgatcgtta tccgcaccac cgagaacatg ctgaaactgg tgccggacag cctgcgtgaa
     4741 gcggcttatg cgctgggtac accgaagtgg aagatgatct ccgcgattac gctgaaagcg
     4801 tcggtgtccg ggattatgac cggtatcctg ctggcgattg cccgtattgc cggtgaaacc
     4861 gcgccgctgc tgtttaccgc gctctccaac cagttctgga gcacggacat gatgcagccg
     4921 atcgccaacc tgccggtgac gatctttaag tttgcgatga gcccgtttgc ggaatggcaa
     4981 caactggcct gggccggggt attgatcatt accctgtgcg tcctgctgct gaacattctg
     5041 gcgcgcgttg tttttgcgaa gaataaacac ggttaacact ttgccggatg cggcgtgaac
     5101 gccttacccg gccctacggt aagcctgatt agcgaaaagc atcaggtacg atgaggaaaa
     5161 gattgcaatg agtatggttg aaactgcccc gagtaaaatt caggttcgta atttgaactt
     5221 ctactacggc aaattccatg ccctgaaaaa catcaacctg gatatcgcca aaaaccaggt
     5281 tacggcgttt atcgggccgt ccggctgcgg taaatcgacg ctgctgcgta ccttcaacaa
     5341 aatgtttgaa ctgtacccgg agctgcgtgc ggaaggtgaa attctgcttg atggcgacaa
     5401 catcctgacc aactctcagg atatcgccct gctacgtgcg aaagtgggca tggtgttcca
     5461 gaaaccgacg ccgttcccga tgtccatcta cgacaacatc gcctttggcg ttcgtctgtt
     5521 tgagaagctc tcccgtgccg acatggacga gcgcgtgcag tgggcattga ccaaagccgc
     5581 attgtggaac gaaaccaaag ataaattgca ccagagcggt tactctctct ctggtggtca
     5641 gcaacagcgt ctgtgtattg cgcgtggtat cgccattcgc ccggaagtgc tgctgctcga
     5701 cgaaccgtgt tcggcgctcg accccatctc taccgggcgt attgaagagc tgatcaccga
     5761 actgaagcag gattacaccg tggtgatcgt tacccacaac atgcagcagg ctgcgcgttg
     5821 ttccgaccac acggcattta tgtacctggg cgaattgatt gagttcagca acacggacga
     5881 tctgttcacc aagccagcga agaaacaaac agaagactac atcaccggtc gttacggttg
     5941 attcaggagt gcgttatgga cagtctcaat cttaataaac atatttccgg ccagttcaac
     6001 gccgaactgg aaagtatccg cacgcaggtg atgaccatgg gcggcatggt ggagcagcag
     6061 ctttctgatg caatcaccgc gatgcataac caggacagcg atctggcgaa gcgcgtgatc
     6121 gaaggcgaca agaacgtcaa catgatggaa gtggcgatcg atgaagcctg cgtgcgcatt
     6181 atcgccaaac gtcagccgac ggcgagcgac ctgcgactgg ttatggtgat cagtaaaacc
     6241 attgccgagc tggagcgtat tggcgacgtg gcggacaaaa tctgccgtac tgcgctggag
     6301 aaattctccc agcagcatca gccgttgctg gtaagtctgg agtcgctggg ccgtcatacc
     6361 atccagatgc tgcacgacgt gctggacgcg ttcgcgcgga tggacattga cgaagcggta
     6421 cgtatttatc gtgaagataa aaaagtcgat caggaatacg aaggtattgt tcgtcaactg
     6481 atgacctaca tgatggaaga ttcgcgcact attccgagcg tacttaccgc gctgttctgt
     6541 gcgcgttcaa tcgaacgtat cggcgaccgc tgccagaata tttgtgagtt tatcttctac
     6601 tacgtgaagg gtcaggattt ccgtcatgta ggcagtgatg agctggataa actgctggcg
     6661 ggaaaagata gcgacaaata attcaccaga caaatcccaa taacttaatt attggga
//
LOCUS       DADMGC010000179         6699 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.179, whole genome shotgun sequence.
ACCESSION   DADMGC010000179 DADMGC010000000
VERSION     DADMGC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.179"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            639..2222
                     /locus_tag="JNZ73_003793"
     CDS             639..2222
                     /locus_tag="JNZ73_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY8733901.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2674..4527)
                     /gene="asmA"
                     /locus_tag="JNZ73_003794"
     CDS             complement(2674..4527)
                     /gene="asmA"
                     /locus_tag="JNZ73_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HAY8733902.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4549..5130)
                     /gene="dcd"
                     /locus_tag="JNZ73_003795"
     CDS             complement(4549..5130)
                     /gene="dcd"
                     /locus_tag="JNZ73_003795"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAY8733903.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(5222..5863)
                     /gene="udk"
                     /locus_tag="JNZ73_003796"
     CDS             complement(5222..5863)
                     /gene="udk"
                     /locus_tag="JNZ73_003796"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAY8733904.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVKVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            6181..6603
                     /locus_tag="JNZ73_003797"
                     /pseudo
     CDS             6181..6603
                     /locus_tag="JNZ73_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422314.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT         1571 a         1744 c         1685 g         1699 t
ORIGIN      
        1 agagtaatta tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt
       61 aattgaataa aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat
      121 tgacagaata attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt
      181 tagctaccaa tacactgatt gagcttaatt tttcaaaccc tctctgcata caatcgttga
      241 tgagaaaggg ttattgcgga aattaacttc cgaatataag gtgacattat ggtaattgaa
      301 tattggctcg ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta
      361 acaattcggc aattggctat ttttaagaat tatattaagt gtcattcaat atggttttta
      421 ggagtttctt taggttgaca atatttaata ttgcgtctcc acatgcgata tttcttaaat
      481 aatgttttat tattaccgct tttaattcag ggataatgtg aggttattac cctcaaataa
      541 tatgagataa atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg
      601 tgacatttgt catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt
      661 ctatctgggc cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc
      721 tggtctttat tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg
      781 ttaccgggct gctgctggcg atgttaatgc gcctgttact gctagcgtca atctcctggc
      841 tggtcaccct gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact
      901 taatcatgct gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac
      961 ggctggaagg gaaagacagc aataatccca cacaacgcaa aggtgcgaag ttctggggcg
     1021 tggtgacgca aattgtggta ctggacgcca ttttctcact cgactcagtg attaccgccg
     1081 tcgggatggt cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga
     1141 tgttgatggc cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc
     1201 tctgcttaag cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg
     1261 tcattccgaa aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcactca
     1321 atcagttggc tatctttaac cggcgacgtt ttctttccgc taatcagacg ctacgccagc
     1381 ggactaccga agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg
     1441 ccgaaaccgc gtccatgctg gtggatcatg gaaaccagca gatctttaat ccgcaggaac
     1501 ggcggatgat tgagcgggta cttaatctta accagcgtac cgtcagcagc attatgacgt
     1561 cgcgccacga tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttgc
     1621 tggagcgaaa tcagcatacg cggctggtgg ttaccgacgg tgatgacgca gaagatttgc
     1681 tcggtgttgt tcacgttatc gacctgctac aacagtcact gcgcggcgaa ccgctcaacc
     1741 tgcgggtgtt gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttaccagccc
     1801 tggagcagtt ccgtaatgcc cgcacgcact ttgcttttgt ggtggatgag tttggctcgg
     1861 tggaagggat tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg
     1921 aagtggaaga gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg
     1981 cgaatggtca tatgccgctg gaggatctgg tgcaatatgt gccgctgccg ctggatgaaa
     2041 aacgtgaata tcacaccatt gccgggctgt tgatggagta tttgcagcgt attccaaaac
     2101 ctggcgaaga agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc
     2161 gcgtgcagaa ggtgcagatt ataccgctgc gtaaggatgg cgagatggag tacgaggtgt
     2221 gatggtatcg gccattttac ccaatgctgc gtaacgcctt ataacttcag caatgaagcg
     2281 ttgtctgtgg taaacggaca tcacagcggg ggtgtgggtc cggctgtaaa tcgccgaatc
     2341 gtttcctgta ggctggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat
     2401 gttacctcgc gcccgtaccc gatagccgaa aggaataaga tgagggaacc gcgccagcgg
     2461 cgatttaccg ccgggagccc gggttgccag ggtggtggcg attgagccac cctggcacgt
     2521 tcaccggctt gagcgttcca tagtagcgag gaacatcaag tgaacggaac agccttcgat
     2581 cttacatatt cagaatataa tagtgccgcc gcgaacgcct gatgcgacgc tttacgcgtc
     2641 ttatcaggcc tacaggacag gtcacctggt aacctacatc ttctccagca acttcttcac
     2701 atctttgcca ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc
     2761 ttcgtcctgt agatgtttgc gcagtaactg atccacttgc aggctgtaat tgagttgctg
     2821 ccagttgcca taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct
     2881 ttccccgttc cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc
     2941 cagattcaac atgccttccc ctgtcagcgc cagcacaggc gattgacctt gcatgtcgtt
     3001 taacgtcacg acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gccgcgtcac
     3061 gttatcgaag ttttcagcgg ccttcacatc accgccatta cgctctaccg cttgctgaat
     3121 catctgctgg aagttcatcc cttccatacg tgtgtcggtc atctcaacat gcgcctgccc
     3181 ttgccagttg tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag
     3241 tgacattttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg taccaatctc
     3301 aacgttctcc agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt
     3361 gcccggcagt gaaacctttc caccgttaag cttgccctgc agttgagtaa tttccagcaa
     3421 acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac
     3481 gttactggcc tgcaacaaaa tatcagctgt aaagccttgc agtccctgat aggccggttc
     3541 atcaatacgc gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc
     3601 ggcaccgttt tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg
     3661 cgggaattgc agtctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgccc
     3721 gctcagcgta ctgtcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga
     3781 ctcctgccac tgggactgga aactcccctg tccctgaata ccttgttttg gtaaatcggc
     3841 accctgcaac tgccagttaa tttgttcaat agccgctgtt aaatcatgcg gataatcaga
     3901 agcatctacc gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc
     3961 ggagaactca aatgagccac gatgttgggg atcttgttcc atttgcaggc ggatattgcg
     4021 gattgtcacc tgctcgtcat cttcatgctg gaacaccaga acgctgtccg ccaccttaag
     4081 actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg
     4141 cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac
     4201 tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc
     4261 cacgtccaga cgcatgttgt cggcgcgaac cagtggctgg cttgcgccct gggcggtgag
     4321 agacattcgc ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc
     4381 gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc
     4441 attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga ccaggagtat
     4501 catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc
     4561 gattcggctg gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta
     4621 aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg
     4681 acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc aaccagacca
     4741 gcccggatcg atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccaaagagga
     4801 acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca gcgtcaccgc
     4861 cagcgccagc tctcctgggt gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc
     4921 gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa
     4981 cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc
     5041 gccgttaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca
     5101 ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgcgc atcactccct
     5161 taacgccaat tacgtcaagg gcataccagc ttatcactga acggtacaca atttatcaag
     5221 cttattcaaa gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc
     5281 cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca
     5341 tcgggcgcac ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt
     5401 taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg
     5461 agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga
     5521 tgaccttctt cggctcaacc ttcaccgttt ctttcatacg cgtatgttca acatagctgt
     5581 aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcaacaggc
     5641 tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat
     5701 ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt
     5761 gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc
     5821 cagcgatacc gataatgacg cactgatgag actgatcagt catatattta gcgacctgat
     5881 taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca
     5941 tccgtctgat accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa
     6001 ttcccggtgg caaattacgt tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt
     6061 gcgcaaatta tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt
     6121 ttgtactagc ataaacacag aaacgaatac tggcgacctg gccttgcgga taaagcggta
     6181 ataagcaaac aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc
     6241 agtttaggtt tagtctcgtt tatctttact cttgtctcgc ttgagctttc gcagtttggc
     6301 acccaactcg ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat
     6361 gccgggcgca tgtggccggg aattgcgctg agctgttcgc tgggaaatat cgccgcatcc
     6421 atcctgcttt tttccaccag ctcgctgaac atgacctgga cgaccatcaa tattgttgaa
     6481 gccgtggtcg gggcagtgct gctacgtaaa ttgctgccgt ggtataaccc cttgcaaaat
     6541 ctggctgact ggctgcgtct ggcattcggc agcgccattg ttccgcctct gttggggggt
     6601 gcccctatta agtagtcaag catgttatga cccctgcggg gtataaaaag tcccgtcgcg
     6661 catcatggcg aacagaacgt cgcagcgtcg tctcgccag
//
LOCUS       DADMGC010000180         6588 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.180, whole genome shotgun sequence.
ACCESSION   DADMGC010000180 DADMGC010000000
VERSION     DADMGC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.180"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(259..441)
                     /locus_tag="JNZ73_003798"
     CDS             complement(259..441)
                     /locus_tag="JNZ73_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733905.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNSEDN
                     DEAASDYALKINESSD"
     gene            772..1644
                     /locus_tag="JNZ73_003799"
     CDS             772..1644
                     /locus_tag="JNZ73_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HAY8733906.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHWGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRGQAREQFTGAVDRIFDTAESVCVASVARKVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1862..4246
                     /locus_tag="JNZ73_003800"
     CDS             1862..4246
                     /locus_tag="JNZ73_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAY8733907.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEDEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNRTLPVDEAWVDDFVREAFGRMKKEYVCKDSEMATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRCRQDGVWGTLCRWANTSDWGW
                     KEYSVDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCTFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            4243..5148
                     /locus_tag="JNZ73_003801"
     CDS             4243..5148
                     /locus_tag="JNZ73_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HAY8733908.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLSGEGTARQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHQKLNHLEEKLHRVDLVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            5145..6215
                     /locus_tag="JNZ73_003802"
     CDS             5145..6215
                     /locus_tag="JNZ73_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAY8733909.1"
                     /translation="MSIEIKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRSVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISIINGFDNPMTQPLLSDGPLTALMDHYLDTDALAGGLPLYVSLYP
                     TEGGMQDIIDCIRAELGSGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVSHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALSRSETVL
                     QKSLEITEEADLALRSAMARIK"
     gene            6430..>6588
                     /locus_tag="JNZ73_003803"
     CDS             6430..>6588
                     /locus_tag="JNZ73_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY8733910.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTH"
BASE COUNT         1679 a         1587 c         1770 g         1552 t
ORIGIN      
        1 gttaccgata tgtgtaaatc tttcaacgga tacgacatat gctgaaggag gatctgtaat
       61 ggattaagtg ataacagata ctcagaaata taggggcaaa tccactattt tgatgagaat
      121 tattatatgt tacctcggat tttaatcagg cgccaagatt aaataaaata ttgagcgcct
      181 gattatattt attcattcat tcattcattc attaattaat atctgttatt gattggctta
      241 ctatcagctt cccccgtatt aatcagagct ttcatttatt tttagtgcat aatcagatgc
      301 cgcctcatca ttatcctcac tattatcata gccgaaaact tcccccggac acgggcgttc
      361 acgataatag tcatcgtcat agttttcatc gtcagataat gatatgtcgt attctgaata
      421 tgattcacca caattataca tgaaatcatc tcctttgggg tggttacaat aattagtgag
      481 tttattcgaa aaaatgatga gcgccagacc aacccaccca acttttgatt ataatcatga
      541 agatacttgt ctcagtacaa atagaatcaa actcagtgca gcatgattca cctttatatt
      601 ctctcaggga aaccaaatac cactactcaa ctattaagaa caaaattctg gaggtatgat
      661 tatctgaaga tacctgtatc ctaaataatt tcagacagat attatcgttg tgagtccccc
      721 gccacctttc cggtgcgcgg ggttttgtct tttttcaccg ggaatacatg tatgaatccg
      781 tctgatgcca ttgaggcaat tgaaaaaccg ctctcctccc tgccttactc actttcccgt
      841 cacatcctgg aacatctgcg caaactcacc agtcacgaac ccgtgattgg cattatgggt
      901 aaaagcgggg ccggtaaatc ctcactctgt aatgcactgt ttcaggggga ggtcaccccg
      961 gtcagtgatg ttcacgccgg cacccgggaa gtacggcgct tccgtctgag tggccatggt
     1021 cacagcatgg ttatcactga cctgcccggg gtgggcgaga gccgggacag ggatgcagag
     1081 tatgaggccc tgtaccgtga cattctgcct gaactggacc tggtactgtg gctgattaaa
     1141 gccgatgacc gtgccctgtc tgtggatgag tatttctggc gacacatcct gcactgggga
     1201 catcagcggg tactgtttgt ggtgacgcag gccgacaaaa cggagccctg ccatgaatgg
     1261 gatatggccg gtattcagcc ttctcctgca caggcacaga atattcgcga aaaaacggag
     1321 gcggtattcc gtctgttccg gcccgtacat ccggttgtgg ccgtatcggc ccgcactggc
     1381 tgggaactgg atacgctggt cagtgcgctc atgacagcgc ttcccgacca tgccgccagt
     1441 cccctgatga cccgcctgca ggacgggctg cgcacggaat ctgtccgggg tcaggcccgt
     1501 gaacagttta ccggtgcggt ggaccggata tttgacacgg cggagagcgt ctgtgttgcc
     1561 tctgttgccc gtaaggttct gcgcgctgtt cgtgacaccg tggtctctgt tgcccgcgcg
     1621 gtatggaact ggattttctt ctgaacccgg tggtgatgca ccagactctt gttacagatt
     1681 tttaacccca tgaaaacaca atgcaaacac agccataccc gggttgtccc cttgcgttgt
     1741 gtttctcaag ttaatagaat gcatcgatcg ttactatcat atcgatcgct ataaacgatc
     1801 gtttgtggta aatgacctcc tccgtggttg tcatgaaaat gcgtctcagg aaggttaaat
     1861 aatgcacgaa aagaacatcg ccctgctttg tgatgaagcc gaccgacttt tgcaactgaa
     1921 cattaatctg ctccggcaaa tggttgatga accggatgtg ttatctgaca gtaagaacga
     1981 aaacagactg ctttttgata aacagaaagc actgaaaaga attgaggagc tggagggcga
     2041 acaaatcaaa accgcccgca gggagatggt gctggctgtt gtcggcacga tgaaagcagg
     2101 taaatcaact accatcaacg ccattgtggg gcaggaaatt ctgcctaacc gtaaccgccc
     2161 catgacctct gtaccgacgc tcatccgcca cgttcccgga aaaactgagc cggtcctcca
     2221 tctggaacat attcagcccg tccgcaattt attaagcaca ctgcaacaaa aactcgacac
     2281 cccggcagga cagcaagtcg cacagaccct gcagcaaacc ggggatacct gcgaactgct
     2341 ggatattctg gcaaatgatg gctggctcaa aaatgaatac cacggggaag acgaaatctt
     2401 taccggactg gcatcgttaa acgatctggt tcgccttgct gcggcaatgg ggagtgagtt
     2461 tccttttgat gaatacgcag aagtgcagaa actgccggtg atcgacgtgg aattcagcca
     2521 tctggtgggg atggatgcat gccagggaac actcacactg ctggataccc ccggccctaa
     2581 tgaggccgga caaccgcaga tggaagtgat gatgcgggat caactgcaga aagcctctgc
     2641 ggtgctggcg gtgatggatt acacccagat gaactcaaaa gcggatgaag acgtccgtaa
     2701 agagcttaat gccattgctg acgtatcagc cggccgcctg tttgtgctgg tcaataaatt
     2761 tgatgagaaa gaccgcaatg gcgatggggc agatgccgta cgccagaaag ttccggcaat
     2821 gctgaacagc gatgtgctgc ccgcctcccg cgtttatcca ggctcatcac gtcaggcgta
     2881 cctggctaac cgtgcgcttc atgagttacg gaaaaacaga acccttcctg ttgatgaagc
     2941 ctgggtcgat gattttgtca gggaagcctt cggtcgcatg aaaaaagaat acgtctgtaa
     3001 agacagtgaa atggcaaccg aaggggcgac agacctgtgg gaaggctcac ttatcgatca
     3061 actgataacg gaagtcatac agagctccca ttccagagcg gcggcactgg cggttgattc
     3121 tgccgccgca aaactgatgc agaatgcgga aaatgtcagc gaatatctgt cattacgcca
     3181 tcaggggcta cagcaaagca ttcagtcact gcagtcgcat atcaccagcc tgcttgcgga
     3241 tatccgggaa atcgcggact gtcaggagca ggtgaccgct gatgtcagaa tggccatgga
     3301 ggagatcgat gccagaaccc gggaattact gacgggggtc tgcacctcac tggaagagga
     3361 gctgaatgac tatttcagaa gcggtaaacg caaagaacag caaatgctgg aggaagaaaa
     3421 ttcagcgcag ccccgggaga gaaacgcatt cgctttcttt cacgatattt tcggaaccgg
     3481 aaatcagcat gacaggatgc gggactttga tccggacagc cccgaaataa aattcagtga
     3541 tcgcagggaa gcccttgaac tgatgacgca aatcgaatcg accgtgacca gcctgcaccg
     3601 tgaggctgaa gcacagttcc gtcctgaact ggaaaaaatc gtcaggggta ttgaaacagg
     3661 ttttcgtggt acggccctgt acgccacaga aaatattgcc ggacgcatca atgcccgtct
     3721 ggaggatgaa ggtttcactg taaaaatcag ttttccggca gtcagccagt tacagacccg
     3781 gctcgcagta aaaacaaatc tgagcgcgct tatggaggaa agaacggaga cagtaacccg
     3841 tcgctgtcga caggatggtg tctggggtac gctttgtcga tgggcaaaca ccagtgactg
     3901 gggctggaaa gagtacagtg tggatgtgag ccgcagcgtg atcaatatca acaaggtcag
     3961 agaggaagtg atgtcactga cccgggcata tttcggggag ctgcaggcat ccattgagca
     4021 ggatattaac cagcccgtcc gccaggaaat cgatgccttt ttctgtacat tcagggagaa
     4081 agttgaacag ctgcgtaaca cgctgattca gagctctgaa gatcataaac gcgatcagca
     4141 ggcgcaggaa cggctgaccg ggcgtcttca ggcgttaaat gaaagggttc ctgagctcat
     4201 tactgacagt aaggcgttaa gggaagaact ggagacaatg ctgtgacttc accatttatt
     4261 caacaaattg ctgataaccg ggtatgtcag gtactcacct gtcttcctga aaaatttgtg
     4321 gttgatttcg caaacggtat tgatgttgca caggagcata tccgtacggc cggagagcgc
     4381 acgttcttcc ggcggttaaa agaggggctg agcggcgaag gtactgcacg gcagaatgcc
     4441 atcaatgctt cgcttgcaca gggcgttgag gcgtccctgc gctggctgac ggagctgaca
     4501 acgtcacttg ccaccacaaa ctacgcgatt acccgggtaa acgacagggt cagttcactg
     4561 gtcagtgata ctgccaggct ggcgcattat tccgcagata cgcgggagca gttactcacc
     4621 ctggccgatc aggttcacca gaaactgaat catcttgaag aaaaactcca ccgtgttgac
     4681 ctggttcagc gggcgcagtt acatcttgag cagatattct cctggtggag cgccgggcga
     4741 tatgcgtcat tttcccctgc cggacgttgt tatgtggcac ttgaagagct tcgctggggc
     4801 gcgtttggtg atgtgatacg tcagggcgaa acaggccagg ttaaccagct actggatatt
     4861 ctcagacata aagcattaac gcagatggca caggagagtg gcggtagtgc aaccgtgcgt
     4921 ctgaacactc tggactggct tggtggtcag ggccgggagc aggcagataa cgagtggcat
     4981 gacgcgatta actggctggg ggactggtgc agtgaagagc agcatccggt gatctggtca
     5041 accacgcagg ctgcagaaca cttaccggtt cgcatgcccc gtctctgctc tgcagaacgc
     5101 ctctctgaaa gtatggtcga tgaaatattt cagaaggggg ctgcatgagt atagaaataa
     5161 aaacagggct ggtgctgtcc ggcggtgggg cggtgggcgc ttatcaggcg ggagtggtta
     5221 aggcactggc agagtgtggt acacagatca gcatggtttc aggggccagc attggcgcat
     5281 tcaatggtgc cattatcgcg gcctctcccg atctgtcaga agctgccgta cgcctggagg
     5341 cgctctggga tcatcttggg aataatcagg tgctgtcggt aaaccgatcg gtttactttt
     5401 cattgctgaa aaaattgttc caggcaatga acctctgcca gatccccgga cgtgcaggag
     5461 cactgcttac gacacttctt cgccatatat cgataatcaa cggcttcgac aatccgatga
     5521 ctcagccgtt gttgtcagat gggcccctga cagcgttgat ggatcattat cttgatacag
     5581 atgctctggc aggcgggcta ccgctgtatg tgtcgctgta ccccacagaa gggggcatgc
     5641 aggatattat tgactgcatt cgtgctgaac tgggttccgg aaccacgaaa aacgccgttt
     5701 ttcagcatat ccagagtctg ccccgcggac agcagaaaga ggctctgctt gcgtcagccg
     5761 cactgcccct gttgttccgt ccccgtgagg ttcaggggac aatgttcggt gatggtggta
     5821 tgggaggatg gcgaaatatg cagggaaata cccctgtaac gcctctggtc gatgccggat
     5881 gcaatatggt gattgtgtcg catctgagtg acggttcttt atgggatcgc caggcttttc
     5941 cggacaccac aatccttgag atccgtcccc ggaaaaggct gaaatatgca ggtgatggtg
     6001 gcaacagtgg cggtctgctc agtttcacgt cggcacatac cgacgcctgg cgtcagcagg
     6061 gctacgagga cacgatgctg acgatggagc atatccggaa accgctggca gcacgtcagg
     6121 cactgtcccg gtcagaaacg gtgttgcaga aaagtctgga gataacggaa gaggcagatt
     6181 tggcactgag aagcgcgatg gcccggatta aataaagacg ccccaaagaa acgccaccgc
     6241 ctgtggtggt gtttttcctg tctggtgttg ttgaaaatat tttcagatat atattacctg
     6301 ggtgagattt aagattaacg catttattaa tgtcataagg agagttatca tgaaattcat
     6361 cgtaaaagca cttaggtgat gctgccaact tactgattta gtgtatgatg gtgtttttga
     6421 agtgctccag tggcttctgt ttctatcagc tgtccctcct gttcagctac tgacggggtg
     6481 gtgcgtaacg gcaaaagcac cgccggacat cagcgctatc tctgctctca ctgccgtaaa
     6541 acatggcaac tgcagttcac ttacaccgct tctcaacccg gtacgcac
//
LOCUS       DADMGC010000181         6311 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.181, whole genome shotgun sequence.
ACCESSION   DADMGC010000181 DADMGC010000000
VERSION     DADMGC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.181"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            85..315
                     /locus_tag="JNZ73_003804"
                     /pseudo
     CDS             85..315
                     /locus_tag="JNZ73_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
     gene            complement(584..2089)
                     /gene="glpD"
                     /locus_tag="JNZ73_003805"
     CDS             complement(584..2089)
                     /gene="glpD"
                     /locus_tag="JNZ73_003805"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAY8733911.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSIGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            2279..2605
                     /gene="glpE"
                     /locus_tag="JNZ73_003806"
     CDS             2279..2605
                     /gene="glpE"
                     /locus_tag="JNZ73_003806"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HAY8733912.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            2650..3480
                     /gene="glpG"
                     /locus_tag="JNZ73_003807"
     CDS             2650..3480
                     /gene="glpG"
                     /locus_tag="JNZ73_003807"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HAY8733913.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            3497..4255
                     /locus_tag="JNZ73_003808"
     CDS             3497..4255
                     /locus_tag="JNZ73_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HAY8733914.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(4237..5835)
                     /locus_tag="JNZ73_003809"
     CDS             complement(4237..5835)
                     /locus_tag="JNZ73_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
                     /protein_id="HAY8733915.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPCGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            6024..>6311
                     /locus_tag="JNZ73_003810"
     CDS             6024..>6311
                     /locus_tag="JNZ73_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAY8733916.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENL"
BASE COUNT         1455 a         1632 c         1687 g         1537 t
ORIGIN      
        1 ccaaagggtc ccgttttttt cgccatcatt tcggaaaagt gtccagaagg ggcaagggga
       61 gtataatttc gccattcaga aagaatggaa agaataatgc aaaatagaaa atggattttg
      121 acctcgctgg taatgacttt tttcggcatc ccaatactga cgcaattttt tgcagcggtt
      181 attgccatgc tgggggctgg gcttgccgct attcctgagg tttgcaattt actctttacg
      241 ccaacaattt accttctgct caatattttt atgctgacgc tgggcgcatt aatgctattt
      301 ttctcggggc gagtggggaa cgtgcctgag ttttgttacg ttgggtatga tggcgtgggc
      361 tttgcggtaa ttccctgaat taaaaagtag gttagatttt tgacaaagtg cgctttgttc
      421 atgccggatg cgacgtgaac gtcttatctg gcctacaaga ccatgcaaat tcaatatatt
      481 gcataaacca cgtaggcctg ataagcgtag cgcatcaggc agttttgtgt ttgtcatcag
      541 tctcaggcca gattgaaatc tgacctgatc accttacgtt aatttacgac gccagcgaca
      601 acttctgctg cgtatattcc accagccact gactcacacg agattgttga tccgcattca
      661 gccacatgcc ttgttttgtg cgacgccaca gggcatcgtc ggtgcggcgg acccattcgt
      721 gatccaccag gtatttcagt tccgcttcgt agaactcatg accgaaatct tccccgagat
      781 cgcttaccgt tcccgcattg ccgagcagca gctcgctgtt gctgccgtaa gtgcgcgcgt
      841 agtgacgtgc cagcgattca gtcaggaacg ggtaacggcg gcgcaggcga gcggcgtaat
      901 cgtcgcggtc gccttcaatg gcaccacccg gcagcacgct ctctttcgtc cacgccgggc
      961 caataccctg ataatacggc gtcagttttt ccagcgcatg ttctgccagt tttcggtagg
     1021 tggtcagctt accgccgaat accgacagca gcggtgcttt gccgttttca tcatggatat
     1081 caagggtgta atcacgggta atggcttgcg gcgagtcgga ctcatcatca cacagcggac
     1141 gcacaccgga gtaggtccag acgatatcgt cacggcttag ctgcttttta aagtgcgcgt
     1201 tatacacttt cagcaggtaa ttgatttcac tctcttcaat cttcactgct ttcggatcac
     1261 ctttgtattc cacatcggtg gtgccgatga tggaaaactc gtccatccac gggatcacga
     1321 acacaatgcg tttatcttcg ttttgcagaa tgtaggcctg cttttgggta tgcacgcgcg
     1381 gcaccacaat atggctgcct ttgatcaggc gaatgccata aggcgaaggc agatgcatcc
     1441 cctcgtcgaa gaactgtttc acccacgggc cggtggcgtt aaccaggccg cgcgcttgcc
     1501 agctatattt tttgccggta tcgatatctt ccgcttccac aatccacagg ccgttttcgc
     1561 ggcgagcaga ggtggcgcga gtccgagtaa gcacttcgcc gcctttacgc accaccatct
     1621 gggcgttggc gagtaccaga cgggcgtcgt ctacccaaca gtcagaatat tcgaatccgc
     1681 gcttaatttc cggttttaac actgaatttg cgccaaaacg caaaccaatt gatcccggca
     1741 ggctggtgcg tttacccaga tgatcgtaca taaacagacc aatgcgaatc atccacgccg
     1801 ggcgcagatg cggacgatgt ggcaggcgaa aacgcatcgg gaaggcgata tgcggagcca
     1861 ttttcagcag cacttcacgt tcagccagcg cctcgctgac caggcggaat tcatagtgct
     1921 caaggtagcg caggccaccg tgaatgagtt ttgaactggc ggaagaggtc gcgcaagcga
     1981 gatcctgcgc ctccagcatc agcacggata aaccgcgtcc agcggcgtct gccgcgatac
     2041 cagcaccatt gatgccgcca cctatcacaa tcagatcttt ggtttccatg ctgccctcat
     2101 tcactttcgt taaagctcat aaatgttcgt tatcgaacat attagcaaag aatcgcgctt
     2161 tgggtaacat tgaaaaaaca ttttagagtg atatgtataa cattatggcg tttatctgcc
     2221 gcttcgacgt aaactgtgcg gtaaatttgc ccacttgttt gtaaagaaag agagacgcat
     2281 ggatcagttc gaatgtatta acgttgccga cgcgcaccag aagttgcagg aaaaagaggc
     2341 ggtgctggtc gatattcgcg atccacagag tttcgctatg gggcatgcgg tgcaggcttt
     2401 ccatttaacc aacgacacgc tgggcgcgtt tatgcgtgat aacgactttg acactccggt
     2461 gatggtgatg tgttatcacg gcaatagcag caaaggggcg gcgcagtatc tgctgcaaca
     2521 gggctacgat gtggtctata gcattgacgg cggctttgaa gcctggcagc gacagtttcc
     2581 cgcagaggtg gcgtacggcg cgtaacgctt tatactgtcc ccttttgtgt ggaataagcg
     2641 acagcaacga tgttgatgat tacctctttt gctaaccccc gcgtggcgca ggcgtttgtt
     2701 gattacatgg cgacgcaggg tgttatcctc acgattcaac aacataacca aagcgatgtc
     2761 tggctggcgg atgagtccca ggccgagcgc gtgcgggcgg agctggcgcg ttttctcgaa
     2821 aacccggcag atccgcgtta tctggcggcc agctggctgg caggccatac cggcagtggc
     2881 ctgcattatc gccgttatcc tttctttgct gccttgcgtg aacgcgcagg tccggtgacc
     2941 tgggtgatga tgatcgcctg cgtggtggtg tttattgcca tgcaaattct cggcgatcag
     3001 gaagtgatgt tatggctggc ctggccattc gatccaacac tgaaatttga gttctggcgt
     3061 tacttcaccc acgcgttaat gcacttctcg ctgatgcata tcctctttaa cctgctttgg
     3121 tggtggtatc tcggcggcgc ggtggaaaaa cgcctcggta gcggtaagct aattgtcatt
     3181 acgcttatca gcgccctgtt aagcggctat gtgcagcaaa aattcagcgg gccgtggttt
     3241 ggcgggcttt ctggcgtggt gtatgcgctg atgggctacg tctggctacg tggcgaacgc
     3301 gatccgcaaa gtggcattta cctgcaacgt gggttaatta tctttgcgct gatctggatt
     3361 gtcgccggat ggtttgattt gtttgggatg tcgatggcga acggagcaca catcgccgga
     3421 ttagccgtgg gtttagcgat ggcttttgtt gattcgctca atgcgcgaaa acgaaaataa
     3481 ttccagggat ttataaatga aacaaacaca acgtcacaac ggtattatcg aactggttaa
     3541 acagcagggt tatgtcagta ccgaggaact ggtggagcac ttctccgtca gcccgcagac
     3601 tattcgccgc gacctcaacg agctggcaga gcaaaacctg atcctgcgcc atcatggcgg
     3661 cgcggcgctg ccttccagtt cggttaacac gccgtggcac gatcgtaaag ccacccagac
     3721 cgaagaaaaa gagcgcatcg cccgcaaagt ggcggagcaa atccccaatg gctcgacgct
     3781 gtttatcgat atcggcacca cgccggaagc ggtggcgcac gcgctgctca atcacagcaa
     3841 tttgcgcatt gtcaccaaca atctcaacgt tgctaacacg ttgatggtaa aagaagattt
     3901 ccgcattatt ctcgccggtg gcgaattacg tagccgcgat ggcgggatca ttggcgaagc
     3961 gacgctcgat tttatctccc agttccgcct tgatttcggc attctgggga tcagcggcat
     4021 cgacagcgac ggctcgctgc tggagttcga ttaccacgaa gttcgtacca agcgcgccat
     4081 tattgagaac tcgcgccacg ttatgctggt ggttgaccat tcaaaatttg gccgtaatgc
     4141 gatggtcaat atggggagca tcagcatggt tgatgccgtc tacaccgacg ccccgccgcc
     4201 agtaagcgtg atgcaggtgc tgacggacca ccatattcaa ctggagctgt gctgatcctg
     4261 cacggcctcc cacgtcagac caaaacgcgc caggtatttg cgtagccgat ccgcgtcatt
     4321 gacgctggct ttgccctggc gcgaaacatc aaaaagctgt cgtccggcag cagaaagcga
     4381 ctttgcctgg cggcagatag cgataacgtg ttccagttgc atacggtcga agagatcgat
     4441 gttttctgcc tctgcgccca gcaacgccgt aagcgcggag gggcggctct cctgccagtt
     4501 atagcgcaga cggtttatct catcttccac cacgtccaga gtgatgcgtc cgctagtggc
     4561 aaaggtggcc atccgcgtga cgctggcaga aagttcgcga aagttaccgc gccatgtagc
     4621 ctggggagat gttgcaaagg ccagccaggc acgccgcgct tcggtgttaa aacgcacgct
     4681 gtcaccagtg agtgtggcgt ggcgctccac ttcataatcc aggttcggtt caatatcttc
     4741 ctggcgctgg cgtagacccg gcagggtgaa ggtccagaga ttgatccgcg cgtacagatc
     4801 ttcgcggaat ttgccttcgg caaccagctg gcgcaaatcg cgcaccgttc cggcgataag
     4861 ctgaaaatca ctgctcacct ggcgatcgct gccaaacggg taaaaggttt tctcttcaat
     4921 agctttcagc agcattgcct gttcgtctgc gcccagttcg ccaatctcat caagaaacaa
     4981 cattccgccg ttggcgctgc gtaataatcc ttcacgagat tcccgcgccc cggtaaacgc
     5041 gcctttcaca tgaccaaaca gcgtcgacat cgcggtatcg ccgcgcaggg tggcgcagtt
     5101 cacttccaca aacgcgccgc taaactgatg ccgtgcctgt tttaactcga agatgcgtcg
     5161 cgccagaaat gacttacctg cgccggtcgg gccgttaagc agaatcggcg cgcgggattt
     5221 aatcgccact ttttcgatct gctcaatcat gcggttgaag tgggggttac gcgtggcaat
     5281 accggatttg aggaaatcga gcgtttgctg ccgttcctcg gcaaagcggc tggcgatggc
     5341 gttataacgg cttaaatcga gatcgataat cgtcacttca cctgggccgc acggctgttc
     5401 ttttttgcgc ggagttgaag attgtatcaa ccgggcgggt agataacgcg cttccgccag
     5461 cagaaaccag caaatctgcg cgacgtgggt gccggtggtg atgtgaatta aatagtcttc
     5521 tttttctggc tgaaactcgt aaccacgggc gaaatcatgc aggcaggcgt aaacctcttc
     5581 gaaatcccac gggttatgca gttcaatctc aacgcccacc acttctgttt ctggcgaaac
     5641 gctggcgata tcgcgtttaa gcgtttcaaa tagcgagcgc gagcgggcat cgtgcaataa
     5701 ttccagtcga tcgatgacca acgattcttg ctggcatata cagagtgacg gacgccattt
     5761 tgaccagcgc tgactgccgc gtccggcata atccagcacg gtaccgacaa agccaaaagc
     5821 cactgtttta cgcatcttag atatccttat aaaaggcgat atttcattat cggtaaatcg
     5881 gctttgcggg aaatctccgt ttacggacat aaagatataa tctttttaga aacaataaat
     5941 taaaaaatta atctgcttat ttaattttct ggcacgacgg ttgcaattat caggaccgca
     6001 aacaacgaaa agagaaaaac aaaatgaatt acgaattact gaccactgaa aatgccccgg
     6061 taaaaatgtg gaccaaaggc gtgccggtag aagccgatgc gcgtcagcaa cttattaata
     6121 cggcgaagat gccgtttatt ttcaaacata ttgcggtaat gcctgatgta cacctgggta
     6181 aaggttccac cattggtagc gtgatcccga ccaaaggggc gattattccg gcggcggtgg
     6241 gcgtggatat tggctgtgga atgaacgcgc tgcgtaccgc gttaacggcg gcagacctgc
     6301 ctgaaaacct g
//
LOCUS       DADMGC010000182         6241 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.182, whole genome shotgun sequence.
ACCESSION   DADMGC010000182 DADMGC010000000
VERSION     DADMGC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.182"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            7..312
                     /locus_tag="JNZ73_003811"
     CDS             7..312
                     /locus_tag="JNZ73_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8733917.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(388..852)
                     /locus_tag="JNZ73_003812"
     CDS             complement(388..852)
                     /locus_tag="JNZ73_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY8733918.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(849..1724)
                     /locus_tag="JNZ73_003813"
     CDS             complement(849..1724)
                     /locus_tag="JNZ73_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HAY8733919.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1721..2410)
                     /locus_tag="JNZ73_003814"
     CDS             complement(1721..2410)
                     /locus_tag="JNZ73_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAY8733920.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="JNZ73_003815"
     CDS             complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="JNZ73_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HAY8733921.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTHKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="JNZ73_003816"
     CDS             complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="JNZ73_003816"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAY8733922.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="JNZ73_003817"
     CDS             complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="JNZ73_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HAY8733923.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1581 a         1692 c         1548 g         1420 t
ORIGIN      
        1 gttgccatga tttacattga acagcttgaa ttaatccata aaagtggtga tgtgttgtat
       61 ccagtaaaaa taaccagaaa atcatcagga aaaactgcct tccaccttgt tccattcggc
      121 ttaaataaga ctcacgacct tctagaggtg gaagatgctt cagaagcaat tcggctggtg
      181 attgatgaac gtcattccat tcgctgcagt acacttacag caactattac gaataaaaag
      241 ggcaagcgaa ttaagcggac aggtatatac agtatcaagg gtgttaacat aaaagaatac
      301 aacgtcaggt aatggcacag agcggtccgt cagttcaggt ttggctctgt gccaaagatg
      361 ggtcaggtca cacctgatct aataaattta agccattaaa tcagccttaa ccttcaccac
      421 caccttttta atctcacccg gctcgccaac aacgcatccc ggcttatgcg gctctcctgg
      481 cataaacacg gcgaacattc ccggtttcag gatgatggtt tgctcgtttt caatggcgct
      541 acaaagctgg taatcatctt catggtggaa ctcttcacat tggcgcgcag tgcctgccat
      601 gccaaacaga atccgttcct caccgtttaa taacagctgg atatcaatgt attgctcgtg
      661 caattccgct tttttctcga cgggcgattg agtgttaaac gtcatgacat tcataaaaat
      721 attgtcgccc tgtaattcgt aacgacccgg cgctttttct tgcggtctgg cagctaaggc
      781 cagcgttaac gcgtcctgta acgcaggatg taacccagca gacggtaatg actgtacttc
      841 acccatcatc ataatttttc tccctgggcc aacagcgcag ccccaagtaa acctgcatca
      901 tggcggtaat gcgccgccag taaatcaaca tgaaatgccg ctggctcctg cgccagatac
      961 atttccacca acgccagata cccttctgcc agaccaacgc tgccaccaac caccacgcac
     1021 tggcaatcag ttgtggcttt aatatcagcg atcagccttg caagcgtacg tgcggagcga
     1081 tgaatcagtt gctgcgcctg ctcatcgccc tgcccggcgc gtgtgaaaat agttctcgca
     1141 tccgcgccag ccagctcgcc ctgcgctgcc gccgcaatgc cgcgaccaga agcaattgct
     1201 tccacgcaac ctgtgcgtcc acagccgcag actgggccgt gtggatcggc aagcgtatgc
     1261 ccgatgtgcc ccgccagacc gccagggccg gtaagcagtt tgccgccgct cactacaccg
     1321 ccgccgacgc cagtggaaac ggtgataaag accatatcgg ttatatcgcc ttccagcgcc
     1381 tgatactctg cccatgctgc ggcctgcgcg tcgttaatgg caatggtcgg caaattggta
     1441 agttgttcca gcgttttgac taacggaaag tgtagcaatc caccgagatt atgcggattc
     1501 agcgccagca aactgccgtc gcgaataatt ccggttgaag cgatggcaac ccgctgcgca
     1561 tgagcttgca acggagagac taatgcggat aaggcatcac gcaaggcttc tggtgtctgg
     1621 ctggctggcg taggaagttc acgacgatcg cggatctgcc cgtcagcgcc aatcagcgcg
     1681 gcggcaagtt tagtaccgcc gatatcaatc gccagtatgg tcatagcacc gcctttttca
     1741 tcgctgtgtt gtaccactga caaatgtgtt caagacgcgt aatggcagaa ccgaccgtca
     1801 ccgcccacgc gccgtggcgc atcgcatctg ccgcctgagc aggcgtgttg taacgccctt
     1861 cggcaatcac ccgacatccg gcttcgctca acgttttcac cagcgccaga tccggctctt
     1921 ctggcgtttc aggcgtggta tagccagaaa gcgtagtgcc aataatttcg gctcccagct
     1981 tttggcatgc caggccgtct tccggcgttg agcagtcggt catcgccagt aaaccgtgat
     2041 gatggatacg tgccagcagc gtttcaacag gcaccggacg cgggcggtcg gtgccgtcaa
     2101 tggcgataat gtccgcgccc gcctgcgcca gcgcatcgac atcttcaata taggccgtga
     2161 tgcgtaccgg agaatcctcc agatcgcgtt tcacaattcc aataatcggc acgctcacca
     2221 ccgcacgcgt ggcttgcaga tttgccacac cttcgatgcg aatggcaacc gcgcccgcct
     2281 gctctgccgc taatgccatg gctgcgacga tttcgggttt atcgagcggg ctgtcaggaa
     2341 ccggctggca ggagacaatc aggccaccgt tagcagcgat tttttgatcc agttgtgcaa
     2401 gtaacgacat acatcttccc ttagcgaaag gcccggtaca tagaccgggc aacaggatta
     2461 acttttggtt ttgactaaat cgtttttggc gctgccaaat ggcacggcac cgctaaatgg
     2521 tttgccgtcg atagcgtcat gagtacgcaa tgcttccggg cgcagccaac gctgaacgcg
     2581 ggaaggcata tccagaccaa tcagcaggat caccacaaat gtcaggctga acgagagcga
     2641 tgccagcgca gtacccagat ccagacgttg agcgatcaac gcgccgatga ttggggccag
     2701 tgcgccaccc aatgcgccaa cgttgtaggt aaagcccagg cccgctgcac gctggtcggt
     2761 atcgaaataa ccgccaatca gtttcggtaa gatcccggcg atcccttgtc caagcatttg
     2821 ctggaagaac agtaacagac cgagcaccca gacgtttgcg ccgccaatcg caaataccgg
     2881 aataatcagc agctgcgagg ccagcaggct acaaacgtac gctttgtggg ttcccagcca
     2941 gtcaccgagg aagccaccta cgcagcatcc cacggccgcg ccaaagccac taaagaacag
     3001 cacattggcg acagtatgcg ggttataagc cagatcggtt ttcagatacg ttggcagcag
     3061 cgcctgaatc ggccatgagt agaggaaagc aaacaacacg accaccatca gcattacgcc
     3121 cgttggccag cgtttacccg tactttgcac cataaagctg ataaagattg cggcgcataa
     3181 cagcccgaga acagcgacga ttgcagcatt ttgcaggtta ccggcgaagc agaaccacag
     3241 cgcagtagcc gccgccagtg tcattacgat attggcaatg cgatgttcac cacggtagag
     3301 aatatccacc attgtgcgta ctggtgcttt acctgcgtgt ttctctttcc agtcttccgc
     3361 ttccgggatg tttttacgca gccagagagc aaagatgatt ggcaaaatgc cgataaagaa
     3421 cagcgcacgc caaccccaga ccggaaccac caggctatag acctgagcgg caacgacggc
     3481 ccccacagag aagcctgaaa tcaaaaaacc actggctttg ttacgcagat gttttggcca
     3541 gctttcaatg acataggtgg cgctggaacc gtattcaccc gccatcccca tgccgatgac
     3601 cagacgagcg ataaacatgg tgatgtagcc tggcgcaaag ccgcaggcca gcgtcccggc
     3661 cgagaagaga acgatgctgg tgaccattgc cagacgacgc ccgtagcggt cacccatagc
     3721 gccgagcatc aggccgccga accagcgaga gataaaggct gcggagatca gacttgccgc
     3781 ctgcaccgtc gtcagcccga attcaccctg tacttcggtg agtaccaggg cgattaaaac
     3841 gaaatcaaaa ccgtcaagca gatatcccaa ccaggcagcg gaaaatgcgc gccattgtgc
     3901 acggttgaga tggcgatacc acgggatatt ctgggttgta gtactcattg tgagtctccc
     3961 gcggtgggcg atgcccacac gctttggtat gaaaattgta gggtacagat gcgtttattt
     4021 cccctcaccc ggtaggggcg agcgagggga aaacaactca cccgcgctct tgcatcaact
     4081 gctgggccag cgccttcagt tctggcagat atttttcatc taccggtcca aacggtttgc
     4141 ggcacagcgg cacagaaacg acatccatat aatgcaggac agttttcagg ccgcggaata
     4201 cgcccgtttt gatcagtaaa tcaatgactt tgttgcactc agtttgcagt ttctgcgcgg
     4261 tctggatatc gccttctttc agcgccttca cgatcccctg atatcgccag cccatgatgt
     4321 tgtaagtact gccgatacca ccatcagcgc ctgccagcaa gccggaagcg aagatttcgt
     4381 cgtaaccgtt atagagcacc agatcaggat gttcacgacg gatctgttcc atctgataga
     4441 gatcgccaga agtctgtttc agcgcaccga cgccaggcaa cgtaaccagg gtgttgatct
     4501 gatccagggt cagttttacc ccactcaggg ctggaatgtt gtacaccacc atcggcaaac
     4561 catccgctga atcaataatt gcccgatagt gatcgcagtg ttcttcaaag ctgaaaggat
     4621 agtagaacgg cgtgacggcg gagacggcat cgaagccata acgtttagcc gatgccgcaa
     4681 gttgttggct ttcagcggtg ctgacgcaac cgacgtgggc gatgagttta atcttacctt
     4741 tcgcctcttc ggcgacgatt tccagtacct gttcacgctc ggaaaggctt tgtacaaagg
     4801 cttcgccggt cgaaccaccc acgtataaac cgtcgatgcc ctgctgaata ttgaactgaa
     4861 ccaggcggcg cagactcgct ttatccagtg cttgttgttg atcaaaagga gtcaggagtg
     4921 cagccattac gccacgtaaa ttcgttgcca taaatacctc tgaagtgatg cttgtctgat
     4981 aaacgatata cctttatacc tgttatacca gatcaattaa gcaacacccc atacagaaag
     5041 cttataatgc gatctgcttc actaaagtgg cattatttct ttttgttggt ggtctgaccg
     5101 aaagcgtgcc aggtagcaga gacgctgttg agatgcgatt gcaacgcacg atcggcttcg
     5161 tcaggatcat gacggcggat cgcatcaacg atcgcaatat gctgttgata actaacgttg
     5221 ttatgttcgt gcagtgcctg atcggtaacc gttgggcgtg cggcaataag ccagtcgagc
     5281 agggcaacgt ggatcgccat gaagattggg ttaccgggga tctccgccag cacgcggtgg
     5341 aaatcaacgt ctgaacgaat gaatgccgcg ttgttatcca gcgactgact gttgatttcc
     5401 agtgcttttg ccagcaaatc gatttgctca tcggtggcgt gttcagccgc atagcgcacc
     5461 aggctggatt caaagaacag acgtaattgt tcgaaatggg caatcccacc aggatgagaa
     5521 aggaaatctt tcgccatgcc ggaaagctcg ccgatgatgg tgtccgcaga aggtcgagag
     5581 acacgagcgc gttcgccgtt gtttatttgc accagacctt tgcgttttaa cgctgccagc
     5641 gcttcacgca cagaaggacg cccgacgtta aagaacgcca tcagttcgcg ttcagacggt
     5701 aattgttcac cttcgccaaa ttcacgacgg cggatcatct gttccagctc ttcttccacc
     5761 atttcggaga gttttttacg cgccagcggg cggctacgca agttgcgacc aattgcaggt
     5821 gaagaatctt cggtttgcga atcaaatgcg ttcataaggc ccattctgta aggtcagtgt
     5881 gattaacatc atcagtgaca tcctatcaca ggattgaaag taggggaaaa tggcaggttt
     5941 ttctctttgt acctcatcat taccataatt aacggaataa ttaactattg cgaaaaatta
     6001 atgtaacgca gataaacaca tcccgattga attattcata agactattca caagcattat
     6061 gaatattatg aatgtgttct tacaaaataa tcataatcgc atatttttta atgaaaaatc
     6121 acctcaccta caattaaaaa cacgacatcc gcaccataaa tagacttgca aaaaatataa
     6181 catcattgtt ttcaatctgc catttatggg attgaccgtt ttcttttgac acggagttca
     6241 a
//
LOCUS       DADMGC010000183         6171 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.183, whole genome shotgun sequence.
ACCESSION   DADMGC010000183 DADMGC010000000
VERSION     DADMGC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.183"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            171..818
                     /gene="fucA"
                     /locus_tag="JNZ73_003818"
     CDS             171..818
                     /gene="fucA"
                     /locus_tag="JNZ73_003818"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAY8733924.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            846..1994
                     /gene="fucO"
                     /locus_tag="JNZ73_003819"
     CDS             846..1994
                     /gene="fucO"
                     /locus_tag="JNZ73_003819"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAY8733925.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2047..2892)
                     /gene="xni"
                     /locus_tag="JNZ73_003820"
     CDS             complement(2047..2892)
                     /gene="xni"
                     /locus_tag="JNZ73_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HAY8733926.1"
                     /translation="MRSLFLFSQPAIACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKADRPP
                     MPEELHDEMPALRAAFEQRGVPCWSASGNEADDLAATLAVKVTLAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(2913..4280)
                     /gene="sdaB"
                     /locus_tag="JNZ73_003821"
     CDS             complement(2913..4280)
                     /gene="sdaB"
                     /locus_tag="JNZ73_003821"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HAY8733927.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDISIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4338..5627)
                     /locus_tag="JNZ73_003822"
     CDS             complement(4338..5627)
                     /locus_tag="JNZ73_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY8733928.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1570 a         1507 c         1644 g         1450 t
ORIGIN      
        1 agagaacttg cagttattta ctgtgattag ttgagctagg tcacaaaata taagcttgtg
       61 aatttctttc ttgttaatca ggaagaagat ggtgggtaat taaacggcta attcaatagt
      121 gtgaaaggaa caacattatt gccctgtttt gaatcagaga gaggtagcta atggaacgaa
      181 ataaacttgc tcgtcagatt attgacactt gcctggaaat gacccgcctg ggactgaacc
      241 aggggacagc ggggaacgtc agtgtacgtt atcaggatgg gatgctgatt acgcctacag
      301 gcattccata tgaaaaactg acggagtcgc atattgtctt tattgatggc aacggtaaac
      361 atgaggaagg aaagctcccc tcaagcgaat ggcgtttcca tatggcagcc tatcaaagca
      421 gaccggatgc caacgcggtt gttcacaatc atgccgttca ttgcacggca gtttccattc
      481 ttaaccgatc gatccccgct attcactaca tgattgcggc ggctggcggt aattctattc
      541 cttgcgcgcc ttatgcgacc tttggaacac gcgaactttc tgaacatgtt gcgctggctc
      601 tcaaaaatcg taaggcaact ttgttacaac atcatgggct tatcgcttgt gaggtgaatc
      661 tggaaaaagc gttatggctg gcgcatgaag ttgaagtgct ggcgcaactt tacctgacga
      721 ccctggcgat tacggacccg gtgccagtgc tgagcgatga agagattgcc gtagtgctgg
      781 agaaattcaa aacctatggg ttacggattg aagagtaatt tcgtaaagca acaaggagaa
      841 ggatgatggc taacagaatg attctgaacg aaacggcatg gtttggtcgg ggtgctgttg
      901 gggctttaac cgatgaggtg aaacgccgtg gttatcagaa ggcgctgatc gtcaccgata
      961 aaacgctggt gcaatgcggc gtggtggcga aagtgaccga taagatggat gctgcagggc
     1021 tggcatgggc gatttacgac ggcgtagtgc ccaacccaac aattactgtc gtcaaagaag
     1081 ggctcggtgt attccagaat agcggcgcgg attacctgat cgctattggt ggtggttctc
     1141 cacaggatac ttgtaaagca attggcatta tcagcaacaa cccggagttt gccgatgtgc
     1201 gtagcctgga agggctttcc ccgaccaata aacccagtgt accgattctg gcaatcccca
     1261 ccacagcagg tactgcggca gaagtgacca ttaactacgt gatcactgac gaagaaaaac
     1321 ggcgcaagtt tgtttgcgtt gatccgcatg atatcccgca ggtggcgttt attgatgctg
     1381 acatgatgga tggtatgcct ccggcgctga aagccgcgac gggtgtcgat gcgctcactc
     1441 atgctattga ggggtatatt acccgtggcg cgtgggcact aaccgatgca ctgcacatta
     1501 aagcgattga aatcattgct ggggcgctgc gaggatcggt tgctggtgat aaggatgccg
     1561 gagaagaaat ggcgctcggg cagtatgttg cgggtatggg cttctcgaat gttgggttag
     1621 ggttggtgca tggtatggcg catccactgg gcgcgtttta taacactcca cacggtgttg
     1681 cgaacgccat cctgttaccg catgtcatgc gctataacgc tgactttacc ggtgagaagt
     1741 accgcgatat cgcgcgcgtt atgggcgtga aagtggaagg tatgagcctg gaaggggcgc
     1801 gtaatgccgc tgttgaagcg gtgtttgccc tcaaccgtga tgtcggtatt ccgccacatt
     1861 tgcgtgatgt tggtgtacgc aaggaagaca tttcggcact ggctcaggcg gcactggatg
     1921 atgtttgtac cggtggcaac ccgcgtgaag caacgcttga ggatattgta gagctttacc
     1981 ataccgcctg gtaaatgcgc tgatgtgata atgccggata cgatttgcgg cgtatccggc
     2041 tcgccgttac cgtaccaacc gcaattgctg taaattgccg tcgatatgca aatcggtttg
     2101 taagcgggca atatcgcggc acagaaacgc catctcttta tgggtttcta attttttgcg
     2161 ccacttttcg gcaaccgcat ccagattctc atatatccct tccagactct gaaactcgac
     2221 cagcagctgc gtggcgcttt ttggtccgat tcccgcaaca cccggtacct ttgaactgct
     2281 gatccccgca agtccccagt aatcgggcaa ctgctgcggt tgaacgccaa attctttatc
     2341 gataaatggc gcatccagcc aacgtttctg gaagtaatca cgaatacgta atgtcggtga
     2401 aagtaactga cagtagcctt tatctgtcga aacaatcgtt gcctgatgcc cggccagtgt
     2461 cactttgacc gccagtgtgg cggctaagtc atcggcttcg ttgccgctgg ctgaccagca
     2521 cgggacgccg cgttgctcaa aggcggcgcg taatgcaggc atctcgtcgt gcaactcttc
     2581 cggcattggc ggtcgatccg ctttgtaatc tggtaaacgc tgatgacgcc agccgctact
     2641 gcggttttca tcatcaaaaa cggcgaccgc gtgggttggc tggctgtgca taatgagctg
     2701 atcgagcgca tgctggcagg tttcgacaca gggcgaaccc tgaacggcat gaatgcgacg
     2761 gataagattc agtgcatcga caataagcaa atgaacagcc acgataaccc ccttaaagat
     2821 caggcgataa gggtaacatt cgatgccgga acaggctatg gctggttgag aaaacaggaa
     2881 gaggcttcgc aaaacgaggc ctctgggaga gattaatcgc aggcaacgat cttcatcgcc
     2941 aggccgccgc gagaggtttc gcggtacttg gcgttcatgt ctttacctgt ttcgtacatg
     3001 gtttcgataa ctttatcgag gcagacgcgc ggttcgctgg tacggcgcag tgccatacgc
     3061 gcggcgttca ccgctttcac tgctgcaatg gcgttacgct caatgcacgg tacctgaacc
     3121 tgccccgcga ccgggtcaca agtcagaccg aggttgtgct ccatcgcgat ttccgctgcg
     3181 atgcaaacct gcgccgggct tgcgcctaac agttctgcca gaccagctgc cgccatagag
     3241 cacgccacgc caacttcacc ctggcaaccc acttcggcac cagaaatcga cgcgttcatc
     3301 ttataaagag aaccaatggc gctggctacc agcaggtaac gagccagtga gttagcgttc
     3361 acttcgcgga taaacttatc gtagtacgcc agcacagccg ggataatccc gcacgcaccg
     3421 ttagtcggcg cagtcaccac gcgaccgcca gcagcgttct cttcgttcac tgccagtgca
     3481 aacatgttga tccagtcaac aaccgccatc gggtcagtgg tggttttatc ctggctgacc
     3541 agcatccggc gtagtgccgc agcacggcgt ggaacgcgca gtttgccagg caacacgcct
     3601 tcggtggaaa taccgcgctc aataccgcca cgcatgactt cccagacgtt cgccaggtgc
     3661 tgttccagct cttctttact gtgcagcgcc agctcgtttt tcatcatcag gccagagagt
     3721 gacagcccgg tttcctgaca atgtttttgc agatcggctg ctgaactgta cggataagga
     3781 acttcaaccg gtgcgctatc ctgctggcca aaatgctctt catcaacgat aaagccaccg
     3841 ccaatagagt agtaagtctg gctgtaaacg actttatcgc ccgccagcgc ggtaatgcgc
     3901 ataccgtttt catgcagaga aaggttgtct gcatggaagt tcatgcactg atcaaccggg
     3961 aactccactt catgctgacc gtttgccagc atcaggcgac catgagtatt cacatcctga
     4021 ataaaaccgg ggatggaatc gatatccacg gtatccggca ggttacccgc caggcccata
     4081 ataatggaga tatctgtgtg gtggccttta ccagtcagag agagcgagcc gtacacgtca
     4141 accaccacgc gggtcacgtc tttaagcagg ttacgggcaa tcagatcgtc ggtaaattgt
     4201 ttacccgctt tcattggtcc aacggtatga gaactggaag ggccaatgcc gattttgaaa
     4261 atatcgaata cgctaatcat aggaaataca tcgcgttaaa acggaggaag cgccgcccga
     4321 aagcggcgcg aaaggactta gctgaacaga gagtagaaga ttgcggagat tgcaatcagc
     4381 cccatcacga caacgaatac gttgctgatg tgaccgctgt acttacgcat tgccggtact
     4441 ttctgaattg cgtacatcgg catcaggaac aggatcatcg cgatgattgg gccgcccagg
     4501 gtttcaatca tacccaggat gctcgggttc agggtggcaa caatccaggt cgttaccagc
     4561 atgaacagcg cagtgatacg gttcagcttg ttgatttcga tagacttacc tttaccacgc
     4621 agagatttaa tcaccatacc gttgaagcct tcacgtgcgc ccaggtagtg gccgaggaag
     4681 gatttggtga tagcgataat cgcgataatc ggagccatcc acgcgataac cggtgcgtta
     4741 aagtggttag ccaggtaaga cagaatcgag atgttctgct ctttagccgc agccaggtct
     4801 gccggagtca ggctcagtac gcagctgaag acgaagaaca ttacggtcag caccatcatg
     4861 atgtgtgcga atgccaggat cttcgagcat ttctgttctg ccatatcgcc gtactcttca
     4921 cgcttcgcaa cggcgaaaga agagatgatc ggagagtggt tgaacgagaa caccattacc
     4981 ggaattgcca gccacagggt catccacaga ccgtttccgg ttgcagatgc agtgtccaga
     5041 gacagcgttt ccagtgctgc gccgttccac tgcgggatca ggtacagagc cagcagcatc
     5101 agtacgccaa caaacgggaa taccagaata ctcatcgctt taacgatcat ctgctcacca
     5161 aagcgaacga tggtcatcat acccacgatc aggatcagcg acagaatcgc acgcggcggt
     5221 ggcgtcatac ccagctggtg agacatgaag ctttcaacgg tattggtgat tgccacgcta
     5281 taaaccagca ggatcgggta gatagcgaag aagtagagca gggtaatcag tttacctgcg
     5341 ccaataccaa agtgttcttc tacaacctcg gtgatgtctt cgcccgggtt tttaccagac
     5401 agtacgaagc gagtcaggcc gcggtgagca aaaaacgtca tcgggaacgc aaggatagcc
     5461 atgatgatca gcgggatcat accgccaaca ccggcgttga ttggcaggaa cagcacgccc
     5521 gcgccgattg ccgtgccgta aaggcccagc atccacatgg tgtctgtctt gcgccaggca
     5581 ctacgagagt ctttcgacgc aatcgtgctg gtttgagtcg tttccatcta tttctcctgg
     5641 aggaatgcaa ataattcagc ttttagccag ttcagcccgg gaaggtgcct gaccgtaaaa
     5701 atgaaatctg tctatcggcg atggcttaat catttaccgc tgtaataatt tgagggcgga
     5761 aagatacaca caagttatgt atctatcagt gatcttgatc ccaaatacaa taatctgctt
     5821 ggaagataga ttatcccaac gctttactct gtaaatcaaa agtaaaagag aatttaaggg
     5881 caatatttgc ccattaaacg acatctgact tcttccgggg cgcgcaatat atgtaggttg
     5941 aactgaaaaa atcaatactt ttagttaatc atggcgataa tttctttttc atagcgataa
     6001 aatacggttt atgaaaatag ggaaacgttt gcgttgcaac ataaattgta tggcatatta
     6061 actgtgatga tatttgtcgc gtgagggtgg taagaaacat tcatataaca aatgaaatga
     6121 gttgatgagt aagtgaccaa aaacgagaaa acccggcaac aaacgtcacc g
//
LOCUS       DADMGC010000184         6001 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.184, whole genome shotgun sequence.
ACCESSION   DADMGC010000184 DADMGC010000000
VERSION     DADMGC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.184"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..92)
                     /locus_tag="JNZ73_003823"
     CDS             complement(<1..92)
                     /locus_tag="JNZ73_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733929.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHG"
     gene            complement(345..1694)
                     /gene="lysC"
                     /locus_tag="JNZ73_003824"
     CDS             complement(345..1694)
                     /gene="lysC"
                     /locus_tag="JNZ73_003824"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HAY8733930.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            2219..3868
                     /gene="pgi"
                     /locus_tag="JNZ73_003825"
     CDS             2219..3868
                     /gene="pgi"
                     /locus_tag="JNZ73_003825"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAY8733931.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4330..4572
                     /locus_tag="JNZ73_003826"
     CDS             4330..4572
                     /locus_tag="JNZ73_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733932.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAVTGGGDGSNTGTTTTTTTSTQ"
     gene            4686..5324
                     /locus_tag="JNZ73_003827"
     CDS             4686..5324
                     /locus_tag="JNZ73_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY8733933.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5321..>6001
                     /locus_tag="JNZ73_003828"
     CDS             5321..>6001
                     /locus_tag="JNZ73_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY8733934.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSET"
BASE COUNT         1500 a         1565 c         1542 g         1394 t
ORIGIN      
        1 ccatgcgcaa tccgcgcccg atccagcagt gttttaagtt cacgctgctc gcgttcagtc
       61 atctcttttt gggtaatacg ggggagtgcc atgatggtgc cctcagtgag ccagagagtt
      121 cagtgtacgg gaattcgttt gcgagcagaa ctgataatat tcattttgcc ttctcttaac
      181 tatttatctc tttcgataat taattagaga tgcagataaa aaaattaaag gcaattattc
      241 tccgataaag acgttggtta acagagtaca ggctcgttta ttgatgagca tagtgacaag
      301 aaaatcaata cggcccgaaa tatagcttcc aggccataca gtatttactc aaacaaatta
      361 ctatgcagtt tttgcaccac ctgctcggca tcttcgccgg gcaccaggaa gcacaggtta
      421 tggctggatg cgccgtaaca aatcatgcga atgttgaacg gttccagtac gccgaatacc
      481 tctttgccaa cgccgcaggc ttttgacagg tcattgccaa tcaacgcgac cagcgccaga
      541 ccttcttcca cctccacccg gcacagtgcg gaaagctcca tcagcagaga ttgcgtcagc
      601 aacgtatcgc cagtggaggt tgaaccggtg gtatcaaggg ttaatgccac gctcacttct
      661 gacgtggtga ttaagtctac cgaaatatta tgccgcgcga ggatgccgaa aacttccgcg
      721 aggaagccgc gagaatgcag catattcagg ctgtgcaaag tgagcagagt ctgattgcga
      781 cgaagcgcca gcgcgcggaa cagcggcgga ttttcagttt tattgcacac cagcgtacca
      841 cctgcgcgtg ggtctttgct ggagccgaca aagaccggga tatcgctgcg tactgcgggt
      901 agcaacgttg ccggatgcag tacttttgca ccaaaagttg ccatctctgc cgcttctgca
      961 aacgcgattt catcaatgcg ttttgctgcg gaaactacgc gtggatcggt ggtgtagatg
     1021 cccgggacgt cggtccagat atcaacacga gatgcgtgta aagcctccgc cagcaaggct
     1081 gccgtataat cgctgcctcc acggccaagc gtcgttgtac gacctttatt ttcgctaccg
     1141 ataaatccct gggtgatcac taagccttca ttgaggcgtg ggagcagctg cagcgcggcc
     1201 agttccgcca gcgcggctac atctggctct gcacgaccaa atcggtcgtt ggtacgcatc
     1261 actttacgta catcaaacca ctgtgcctga acatcgcgtt cgcgcaggat ttcgacaaac
     1321 agcagggtcg acatcagctc gccgtggctg accagctcat ctgtcagcgc cggagacgtt
     1381 gccagcgccg ccgcttctgc cagaacagta atgttctcca gcagacgttc aatctcttca
     1441 cggataacgt tcgggtaacg cagacgttcc agaatggcaa actggatgtt gcgaatagcg
     1501 tcgagttttt cgaatcgctc gccaggttcc agcccttccg ctaaagcgac cagcagatta
     1561 gtgataccag cagaagccga gaggactact aaacgcacgt tggcatcaga aagcacaata
     1621 tcagcgctgc ggttcatggc gtcaaaatca gctacgctgg taccgccaaa tttggagaca
     1681 acaatttcag acataactac ctcatgtcag gggatccatt ttcagccttg gcacaaggga
     1741 agagcggaag acgggtgggc gcagagcgat acttcgctac tattttcacc cagaagtgct
     1801 ccaccacttg cgaaacgccc gactgcgaac gcttctggtg acaacccagg ggattcagcc
     1861 cctgtagccg atgatgaacg tggccagccg ttcaatcacc tcggcgatgc accccctcag
     1921 gtgttatcac aggactggct cctccaacac cgttacttgg gcaacgcgcc tcttctggcc
     1981 tgcgctagcg caggtagtac atttataaat aaagggtgag cggggcggtt gtcaacgacg
     2041 gggtcatgcg gatttttcat ccactcctgg cggtcagtag ttcagctaat aaatgcttca
     2101 ctgcgctaag ggtttacact caacattaca ctaacggcac taaaaccatc acatttttct
     2161 gtgactggcg ctacaatctt ccaaagtcac aattctcaaa atcagaagag tattgctaat
     2221 gaaaaacatc aatccaacgc agaccgctgc ctggcaggca ctacagaaac acttcgatga
     2281 aatgaaagac gttacgatcg ccgatctttt tgctaaagac ggcgatcgtt tttctaagtt
     2341 ctccgcaacc ttcgacgatc agatgctggt ggattactcc aaaaaccgca tcactgaaga
     2401 gacgctggcg aaattacagg atctggcgaa agagtgcgat ctggcgggcg cgattaagtc
     2461 gatgttctct ggcgagaaga tcaaccgcac tgaaaaccgc gccgtgctgc acgtagcgct
     2521 gcgtaatcgt agcaataccc cgattctggt tgatggcaaa gatgttatgc cagaagtcaa
     2581 cgcggtgctg gagaagatga aaaccttctc ggaagcgatt atctccggtg agtggaaggg
     2641 ttataccggc aaagcaatca ctgacgtagt gaacatcggg atcggcggtt ctgacctcgg
     2701 cccgtacatg gtgaccgaag cactgcgtcc gtacaaaaac cacctgaaca tgcactttgt
     2761 ttctaacgtc gatgggactc acatcgcgga agtgctgaaa aaagtaaacc cggaaaccac
     2821 gctgttcttg gtagcatcta aaaccttcac cactcaggaa accatgacca acgcccatag
     2881 cgcgcgtgac tggttcctga aagcggcagg tgatgaaaaa cacgttgcga aacattttgc
     2941 ggcgctctca accaatgcca aagccgttgg cgagtttggt attgatactg ccaacatgtt
     3001 cgagttctgg gactgggtcg gcggccgtta ctctttgtgg tcagcgattg gcctgtcgat
     3061 tgttctctcc atcggctttg ataacttcgt tgaactgctt tctggcgcac acgcaatgga
     3121 caagcatttc tccaccacgc ctgccgagaa aaacctgcct gtcctgctgg cgctgattgg
     3181 catctggtac aacaatttct ttggtgcgga aactgaagcg attctgccgt atgaccagta
     3241 tatgcaccgt ttcgcggcgt acttccagca gggcaatatg gagtccaacg gtaagtatgt
     3301 tgaccgtaac ggtaacgttg tggattacca gactggcccg attatctggg gtgaaccagg
     3361 cactaacggt cagcacgcgt tctaccagct gatccaccag ggaaccaaaa tggtaccgtg
     3421 cgatttcatc gccccggcta tcacccataa cccgctctct gatcaccacc agaaactgct
     3481 gtctaacttc ttcgcccaga ccgaagcgct ggcgtttggt aaatcccgcg aagtggttga
     3541 gcaggaatat cgcgatcagg gtaaagatcc ggcaacgctt gactacgtgg tgccgttcaa
     3601 agtgttcgaa ggtaaccgcc cgaccaactc catcctgctg cgcgaaatca ctccgttcag
     3661 cctgggtgcg ttgattgcgc tgtatgagca caaaatcttt actcagggcg tgatcctgaa
     3721 catcttcacc ttcgaccagt ggggcgtgga gctgggtaaa caactggcaa accgtattct
     3781 gccagagctg aaagatgata aagaaatcag cagccacgat agctcgacca atggtctgat
     3841 taaccgctat aaagcgtggc gcggttaatc atcatgcgtg cctgatgccg atatgtaggc
     3901 cggataaggc gtttacgccg catccggcaa ccggagcctg atgccaatat cgacattaat
     3961 gcatagtatg aggagttaaa taaccttaaa aacaatgccg acaataagtc ggcattgttt
     4021 tatcagataa atccccttgt ctgtgattta acggaaatca taccgtgagg ttaatcctaa
     4081 aatagatttt taatcgttgt ttatttcgga gaatacgcag attaattact tttgttttta
     4141 ttttaagttt atgattttta ttgttattta aatataagtt gaaacttata tttgatattc
     4201 attccaatta tcctaaaact ccatcgctaa ttccccgcgc cgtaattcgc atgctttagt
     4261 tgtgtatact cgatcccgcc cgaaatgttt ttgggtaaat ctccattcat tcaatgaagg
     4321 gaaattgtta tgaaaaaagt tctgtatggc atttttgcca tatctgcgct tgcggcgact
     4381 tctgcgtggg ctgcacctgt acaggtgggc gaagcggcag agtcggcagc aacgtcggtt
     4441 tcggcgggga gttcctccgc gaccagcgtc agcaccgtaa gctcggcggt gggtgtcgcg
     4501 ctcgcggtaa ccggtggcgg tgatggttct aataccggga ccaccacaac cacgaccacc
     4561 agtacccagt aataaagtat gtatccccaa aataatttga gtcattgcat ctgtggcttg
     4621 aagtatgaag ggatttacca taaccacact ccggtgtggt tattctgccc ctctggagaa
     4681 gagttgtgaa gcgacctgca ctcattctta tctgcctgct attacaggcc tgttcagcca
     4741 cgactaaaga gctgggcaat tcactgtggg acagtctgtt cggcacgcca ggcgtacagc
     4801 tgacggacga tgatattcaa aatatgccct acgccagcca gtacatgcag cttaatggcg
     4861 ggccgcaatt gtttgtggtg ctggccttcg ctgaagacgg acaacaaaaa tgggtcactc
     4921 aggatcaagc cactcttgtc acacaacatg gtcgtctggt gaagactttg cttggcggcg
     4981 acaacctgat tgaagtgaat aaccttgccg ccgacccgct gattaaaccc gcacaaattg
     5041 ttgatggtgc aacatggacg cgcacgatgg gctggaccga gtaccagcag gtacgctacg
     5101 ccaccgcacg ctcagtcttc aaatgggatg gcaccgatac cgtcaaggtc ggcagcgatg
     5161 aaaccctggt tcgcgtgctg gacgaagaag tctccaccga ccaggcgcgc tggcataacc
     5221 gctactggat cgacagcgaa gggcaaattc gccagtcgga acagtatctc ggcgcggatt
     5281 atttcccggt gaaaaccact ctcatcaagg cggcaaaaca atgattaaac aaactattgt
     5341 cgcgttgctt ttgagtgtgg gagcgtcatc ggtcttcgcg gcaggaaccg tcaaggtgtt
     5401 cagcaatggc agcagtgagg ccaaaacgct aacgggcgca gagcatttaa tcgatctggt
     5461 agggcaacct cgactggcaa acagctggtg gcctggtgcg gtgattagcg aagagctggc
     5521 aacggcggca gcattgcgtc agcagcaggc gttgctgaca cggctggcag agcagggcgc
     5581 agattccagc accgacgatg ccgccgccat taacgcctta cgccagcaaa tccaggcatt
     5641 gaaagtgaca ggcaggcaaa aaatcaatct tgatcccgat atcgtccgcg ttgccgaacg
     5701 cggtaacccg ccgttgcagg gcaactacac gctgtgggtc ggaccaccgc cgtccacggt
     5761 cacgttgttc gggcttatca gccatcctgg caatcagcca ttcactccag gtcgcgacgt
     5821 ggcgagctat ctctctgacc agagcctgct cagcggtgcg gatcgcagct acgcgtgggt
     5881 cgtttacccg gacggacgca cgcaaaaagc gccggtggct tactggaaca agcgtcacgt
     5941 agagccgatg cccggcagca ttatttatgt tggcctcgca gattccgtct ggagtgagac
     6001 t
//
LOCUS       DADMGC010000185         5997 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.185, whole genome shotgun sequence.
ACCESSION   DADMGC010000185 DADMGC010000000
VERSION     DADMGC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.185"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..268)
                     /locus_tag="JNZ73_003829"
     CDS             complement(<1..268)
                     /locus_tag="JNZ73_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY8733935.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATE"
     gene            805..2706
                     /locus_tag="JNZ73_003830"
     CDS             805..2706
                     /locus_tag="JNZ73_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAY8733936.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGNVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSFDDSSANLFKLGAENIFLGRKAATKEE
                     AIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVF
                     CQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDE
                     VLELLAGRK"
     gene            2801..3949
                     /gene="mtlD"
                     /locus_tag="JNZ73_003831"
     CDS             2801..3949
                     /gene="mtlD"
                     /locus_tag="JNZ73_003831"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY8733937.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            3949..4536
                     /gene="mtlR"
                     /locus_tag="JNZ73_003832"
     CDS             3949..4536
                     /gene="mtlR"
                     /locus_tag="JNZ73_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HAY8733938.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(4548..4757)
                     /locus_tag="JNZ73_003833"
     CDS             complement(4548..4757)
                     /locus_tag="JNZ73_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733939.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5041..5403
                     /locus_tag="JNZ73_003834"
     CDS             5041..5403
                     /locus_tag="JNZ73_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HAY8733940.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         1567 a         1454 c         1488 g         1488 t
ORIGIN      
        1 gttcagtcgc tttctcctgc gacgcaacat gtgactgcat accccagcca cgctccggtt
       61 gatagagcgt cgcccagatc cacagaaacg gccagataac atgcagagta aacaggctca
      121 cccagcccgc cgtatgaata gcgtcggcat gggggtgatt gcgctttttg gcaatcagat
      181 aagggatgtc atgtatcgcg atgattccat aaaaaatcac cagaaagacg aagatcagca
      241 ctcccaacgc gaaatagttt agaaacatat ctgcctcgga ttcacgttta tcagtgttgt
      301 tttttggctg gcagccagaa gggagtcagg ctgatatttt gacaataatc cgggttcgcg
      361 attctcgcca taacaccaaa gaataatttt tagaggtgat gagttgctta gttacataac
      421 gattgtatga cgaaggcata acatgctgta gatcacatca ggtgaacgcc gtaagaaaat
      481 atcttgtgat tcagatcaca aagattcaac aaaccatcaa aacaaaaatg tgacattact
      541 cacatttaaa tgccattttt agcgaaaatc accgccttgt ttctttttta cacaggcgtt
      601 ttgtgatgag cgtcacgtca attacctctc taccccctat atttatgtga ctgatatcac
      661 acaaaagccc gtcgactgga cagttaaccg attcagtgcc agatttcgca gtatctacaa
      721 ggtccggcta cctctgccgc cacattaaca aaaaacctcg ggcttccagc ctgcgcgaca
      781 gcaaacataa gaaggggtgt ttttatgtca tccgatatta agatcaaagt gcaaagcttt
      841 ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg cgtttatcgc gtggggtatc
      901 atcaccgcgt tatttattcc aacagggtgg ttaccgaacg agacgctggc gaagctggtc
      961 gggccgatga tcacttatct cctaccgctg ctgatcggtt ataccggtgg taagctggta
     1021 ggcggcaaac gtggcggcgt agtcggtgcc attaccacca tgggcgttat cgtcggcgca
     1081 gacatgccga tgttcctcgg ttctatgatt gcaggtccgc tgggcggctg gtgcattaag
     1141 cacttcgacc gctgggtaga cggtaagatc aaatccggtt ttgagatgct ggtgaataac
     1201 ttctccgcag gcatcatcgg gatgatcctc gctattctgg cattcctcgg cattggcccg
     1261 attgttgaag ccctgtccaa aatgctggct gcgggcgtta acttcatggt tgtccatgac
     1321 atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa tcctgttcct caacaacgcc
     1381 attaaccacg gtatcttctc gccgctgggt attcagcagt cccatgaact gggtaaatca
     1441 atcttcttcc tgattgaagc taacccaggt ccaggtatgg gcgtgctgct ggcgtacatg
     1501 ttctttggtc gtggtagcgc taaacagtct gcgggcggtg cggcaatcat ccacttcctg
     1561 ggtggtatcc acgaaatcta cttcccgtat gtgctgatga atccgcgtct gatcctcgca
     1621 gtcatcctcg gcggtatgac tggcgtgttc acgctgacta tcctgggcgg tggtctggtt
     1681 tctccggcat ctccgggttc tatccttgct gtactggcga tgacaccaaa aggtgcttat
     1741 ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct ccttcgttgt ctctgctatt
     1801 ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg aagcagcaac tcgtcgtatg
     1861 caggacatga aagctgagtc taaaggcaca tctccgctat ctgcgggcaa tgtgactaac
     1921 gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg ccggtatggg ttcaagtgcg
     1981 atgggcgcag gcgtgctgcg taagaaaatt caggatgcag gtctgtcgca gatttcggtc
     2041 actaacagcg cgatcaacaa cctgccgcca gatgtggacc tcgtcatcac tcaccgtgac
     2101 ctgaccgaac gcgctatgcg ccaggttccg caggcacagc atatttcgct gaccaacttc
     2161 ctcgacagcg gcctgtacac cagcctaacc gaacgtctcg ttgctgccca gcgccatact
     2221 gaaaacgaag tgaaagtgaa agacagcttt gacgattcca gtgctaacct gttcaaactg
     2281 ggcgcggaga acatcttcct cggtcgcaaa gcagcaacca aagaagaagc gattcgtttt
     2341 gctggcgagc agctggtgaa aggcggttac gttgagccgg aatacgttca ggcgatgctg
     2401 gatcgtgaaa aactgacccc gacttatctg ggtgagtcta tcgcggtgcc acacggtacg
     2461 gttgaagcga aagatcgcgt actgaaaacg ggcgtggtgt tctgccagta cccggaaggc
     2521 gtgcgcttcg gtgaagaaga agatgacatt gcccgtctgg tgattggtat tgcagcccgt
     2581 aacaacgagc acattcaggt tatcaccagc ctgaccaatg cactggatga tgagtccgtc
     2641 atcgagcgtc tggcacacac caccagcgtg gatgaagtgc tggaactgct ggcaggtcgt
     2701 aagtaatcca atcctaccct ctcctcacgg agagggtttg ggtgagggaa aagcctcacc
     2761 ccagccctct cgggtaaaaa cattgatgaa ggttaatact atgaaagcat tacattttgg
     2821 cgcaggtaat atcggtcgtg gctttatcgg taaactgctg gcagacgcgg gtatccaact
     2881 gacgtttgcc gatgtcaatc aggtggtact tgatgccctg aatgcccgtc atagctatca
     2941 ggttcatgtg gtcggtgaaa ccgagcaggt agataccgtt tccggcgtca atgctgtcag
     3001 cagcattggt gatgatgtcg ttgatctgat tgctcaggtc gatttagtca ctaccgccgt
     3061 tggcccggtt gtgctggaac gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa
     3121 agaacaaggt aatgaatccc cgctgaacat catcgcctgt gaaaacatgg tacgcggcac
     3181 cacgcagctg aaaggtcatg tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga
     3241 agagcacgtt ggctttgtcg attccgccgt tgaccgcatc gtaccgcctt cggcttcggc
     3301 aactaacgat ccgctggaag tgacggtaga aactttcagc gaatggattg tcgataaaac
     3361 gcagttcaaa ggcgcactgc cgaacatccc aggcatggag ttaaccgaca acctgatggc
     3421 atttgtcgaa cgtaaactct tcaccctgaa cacgggtcat gctataaccg cgtacctcgg
     3481 aaaactggcc ggtcatcaga ccattcgtga cgctattctc gacgagaaaa tccgcgcggt
     3541 ggtaaaaggt gcgatggaag aaagtggtgc ggtactgatc aagcgctacg gctttgacgc
     3601 agacaaacat gcggcgtaca tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa
     3661 agatgatgta gagcgcgtag gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct
     3721 gatcaagcca ctgctcggta cgctggaata cagccttccg cacaagaatc tgattcaggg
     3781 gattgctggt gcaatgcact tccgcagtga agatgatccg caggctcagg aactggcagc
     3841 actgatcgct gacaaaggtc cgcaggcggc gctggcacag atttccggtc ttgatgccaa
     3901 cagcgaggtt gtatccgagg cggtaaccgc ttataaagca atgcaataat ggtggaccag
     3961 gcgcaggaca ccctgcgccc gaataacaga ttgtcagata tgcaggcaac aatggaacaa
     4021 acccaggcct ttgaaaaccg tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc
     4081 tttctgatca ccgccgtcga gcttttgacc gaggcggtaa atcttctggt gcttcaggta
     4141 ttccgcaaag acgattacgc ggtgaagtat gctgtagaac cgttactcga cggcgacggt
     4201 ccgctgggcg atctttctgt gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc
     4261 caggaatacg aagatgcgga actgctgatg gcattgcgtg aagagctaaa tcacgacggc
     4321 aacgagtacg cctttaccga cgacgaaatc cttggaccct ttggtgaact gcactgcgtg
     4381 gcggcgttac caccgccgcc acagtttgaa ccagcagact ccagtttgta tgcaatgcaa
     4441 attcagcgct atcaacaggc tgtgcgatca acaatggtcc tttcactgac tgagctgatt
     4501 tccaaaatca gcttaaaaaa agcctttcaa aagtaagcaa cgtctgctta ctgcccctct
     4561 acctgcttcg gccgataaag ccgacgataa tactccagac gttgtagata taacggcacg
     4621 ctttcatcgg gtattccgga cggaatcgcg ttacggggag gaagtttttt cagatactcc
     4681 cggaacgcct ggcttgatgc catgaaatct acggctttat cgataagaag cggaacgttt
     4741 tcacctaatt tgcccatgat gatatctccg tattaccccg cgccgggaat gcgcggccgc
     4801 caattttagt ttaggttccc ggtaaagatg acattaagga aaacgtgctg aatcctcaat
     4861 aatcatccgc tgctgtttat tatctttttc tatagatttc ttaattaatc aacgaattgc
     4921 gtagtttttg agcatgttaa tgattacgtt gcagaactat tacaatgtca tcagttgtga
     4981 acaaagcacc tggtcgcgca tactaggggc tataaattta tctttatcag aagccatcac
     5041 atgaaagaag tcgaaaaaaa cgaaatcaaa cgtctcagcg atcgcctgga cgccatccgc
     5101 caccagcagg ccgatctgtc gctggttgaa gccgcagaca aatatgccga gctggaaaaa
     5161 gagaaagcca cgctggaagc agaaattgct cgcctgcgtg aggttcatag ccaaaaactg
     5221 agtaaagaag cacagaagct gatgaagatg ccgttccagc gagcaattac caaaaaagag
     5281 caggctgata tgggcaagct gaagaaaagt gttcgcggac tggtggttgt gcacccaatg
     5341 accgcgctgg ggcgcgaaat gggcctggaa gagatgactg gattttctaa gactacgttt
     5401 taagcccctc agacgactgg ttcgatcgta tcaacccgca tctggcttgc cttagctggt
     5461 caatgcgggg actctcctgt ctactcgcct aattaccaaa caattcactc cctttacttc
     5521 gatttaccgc tggttgatat tctgaaaatg catttttacc gaaatagcca tctataaaca
     5581 ataaaaatat atagaagata tcatttgtta attaacaagc atttacgtct ctttttttcg
     5641 gatttaattg tgaaatttta atcatcatat tcacaaaatc aattatctgt aaaatttttc
     5701 ccagacatgg tttctttcat atttgacatt tattaataat catcattaga atccacgaaa
     5761 aataatatat tcatacaaac acagacatct tttgaaacga atgatacatt tttatgatga
     5821 atgcttaaaa caatatttaa tcgacacaca tcactgtcat taatgtaaag caacatagct
     5881 caccaataat acaatggaca actctaccgg cattgatcgc tggtcggagg aaataaggat
     5941 ttttacatga acttgaagaa gatttttttc agtgctgtca ctgtttcagt tctctgc
//
LOCUS       DADMGC010000186         5974 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.186, whole genome shotgun sequence.
ACCESSION   DADMGC010000186 DADMGC010000000
VERSION     DADMGC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.186"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..109)
                     /locus_tag="JNZ73_003835"
     CDS             complement(<1..109)
                     /locus_tag="JNZ73_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HAY8733941.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIG"
     gene            complement(366..698)
                     /locus_tag="JNZ73_003836"
     CDS             complement(366..698)
                     /locus_tag="JNZ73_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HAY8733942.1"
                     /translation="MEQIQPLKIIKGGAEPGVWGVELLAIRYAAWIKPEFEIEVYEVF
                     KTVVRLGVGAMSRLNRIDHIINTETKAISQCASQMAKWGVGGRKRLLHVARERAANEV
                     QMYLPGMV"
     gene            complement(671..871)
                     /locus_tag="JNZ73_003837"
     CDS             complement(671..871)
                     /locus_tag="JNZ73_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HAY8733943.1"
                     /translation="MKYPTVIVNGVSVRVDEDGRYNLNDLHAAAVANGEATEQQRPSQ
                     FLRSAQIKRFIKALWNKFNHLK"
     gene            complement(1077..1229)
                     /locus_tag="JNZ73_003838"
     CDS             complement(1077..1229)
                     /locus_tag="JNZ73_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733944.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGDHFWSKSRLNISLLMLDIEHLA
                     RGKGLR"
     gene            complement(1217..1684)
                     /locus_tag="JNZ73_003839"
     CDS             complement(1217..1684)
                     /locus_tag="JNZ73_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY8733945.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            complement(1681..2157)
                     /locus_tag="JNZ73_003840"
     CDS             complement(1681..2157)
                     /locus_tag="JNZ73_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HAY8733946.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIDV"
     gene            complement(2141..2464)
                     /locus_tag="JNZ73_003841"
     CDS             complement(2141..2464)
                     /locus_tag="JNZ73_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HAY8733947.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            2689..2907
                     /locus_tag="JNZ73_003842"
     CDS             2689..2907
                     /locus_tag="JNZ73_003842"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733948.1"
                     /translation="MATAQFGLWRPVLISGLLQLPTAGYVATPNPAKDSCPYTSACAF
                     TTTERALANQARRLFTIIGSMLLPVVQI"
     gene            complement(3176..3799)
                     /locus_tag="JNZ73_003843"
     CDS             complement(3176..3799)
                     /locus_tag="JNZ73_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY8733949.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTELWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            complement(3796..3984)
                     /locus_tag="JNZ73_003844"
     CDS             complement(3796..3984)
                     /locus_tag="JNZ73_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HAY8733950.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            complement(3981..4343)
                     /locus_tag="JNZ73_003845"
     CDS             complement(3981..4343)
                     /locus_tag="JNZ73_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY8733951.1"
                     /translation="MSEYQFVLPYPPSVNTYWRRRGNQYYISDKGQKYRKEVQQIILQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENV"
     gene            complement(4340..4630)
                     /locus_tag="JNZ73_003846"
     CDS             complement(4340..4630)
                     /locus_tag="JNZ73_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY8733952.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            complement(4630..5352)
                     /locus_tag="JNZ73_003847"
     CDS             complement(4630..5352)
                     /locus_tag="JNZ73_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY8733953.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEDCGRINGLGLNQSFCVYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEEAAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(5345..5554)
                     /locus_tag="JNZ73_003848"
     CDS             complement(5345..5554)
                     /locus_tag="JNZ73_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HAY8733954.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEHCCAELMSDPNSSMYEEEDDE"
     gene            complement(5514..5915)
                     /locus_tag="JNZ73_003849"
     CDS             complement(5514..5915)
                     /locus_tag="JNZ73_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733955.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMVSIQLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVKNHHAVRQAKRKANTTYMKAYRKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
BASE COUNT         1359 a         1534 c         1356 g         1725 t
ORIGIN      
        1 tgccaatgaa ctgaaatgct tcttctgcgg ctgcgttctg gttcataacc agtttgtaaa
       61 tctctaactg gaatttctgt tcttcagtca tgggaataat ctctgccatt gttggctccg
      121 tttatccgtt aaaagggata tcagttaagt tatcccgtgt agggtataag ccattatcga
      181 gaccactcat tgaatggcct ctgcaataac cgatgtcttt ccatcagtcc gccaccacaa
      241 agaatctttt ttgccataag gctggaggtt catctttcag tggctgccag tgttatttcc
      301 ccacttactg gcttgggttg tttcgcggta ctgccgtaat gcaaaaactg gattaacctg
      361 cgaaatcaca ccattccggg caaatacatt tgcacttcat ttgccgctct ctcacgtgca
      421 acatgaagca atctttttcg cccaccaacg ccccacttag ccatttggct tgcgcactgg
      481 cttatcgctt tggtttcagt attgatgatg tgatcgattc tattcagacg ggacattgcg
      541 ccaacgccga gacggacaac cgttttgaaa acttcataaa cttcgatttc aaattccggc
      601 ttaatccatg ctgcatatct gattgccaga agttcaacac cccacacacc tggttctgca
      661 ccacctttga ttattttaag tggttgaatt tgttccaaag tgcttttatg aagcgtttta
      721 tctgcgcgct acgcaaaaac tggcttgggc gctgttgctc tgtagcctct ccatttgcaa
      781 ctgctgctgc atggagatcg tttaagttgt agcgtccatc ctcatcaaca cgaacggaca
      841 caccattgac aataactgtt gggtacttca tcagtgatta ccttttagtg atgaaccttg
      901 tcacacagga ttccggccca cagaaaggca ccgattacca aaccggcatc ctcaagggtc
      961 atcctgaaag gctctgtgtt cataagtcgc gcgtgtgaag cgcgtttgtt gcagatataa
     1021 aaaagccccg cgaatgcgag gctaaatcct ggtgtttgtg atgactggct cttatctcaa
     1081 cgcagcccct taccgcgcgc cagatgctca atatcaagca tcagcaatga gatgtttaat
     1141 ctggatttac tccagaagtg atcacccccc tgtctacaga gccagatgtg aaggatgatg
     1201 agtaaaatta tcgctatcat cgaaggcatt gcgtcctgat gtattcctga agcgttctca
     1261 gtgctgtttg gtcgcggata attccgtccc ggataccgag aacgtttcgt ccagcaactg
     1321 gagagagttc gacggtggca tcattgccca tgccggaggc gctggaggtt tcggctgagg
     1381 atggcacagg gcattttcct ttgacgagca ccctgccacc attatcaagc ttgcgccgaa
     1441 gagcatcatt ttcagctttc gcatcagcta actccttcgt gtatttagca tcgagtgcat
     1501 cagcagcacg ctggcgttgc tgcatgtcag taatggtggc ggtcgcctgc ttcagctcac
     1561 tgactttttt atctcgctgc tctttgtagg tcatggcgtt atcacggtaa tgattaaccg
     1621 cccatgacag gcagacgatg atgcagataa ccagagcgga gataatcgcg gtgactctgc
     1681 tcatacatca atctctctga ccgttccgcc tgcttctttg aattttgcaa tcaggctgtc
     1741 agccttatgc tcgaactgac cataaccagc gcccggaagt gaagcccaga tattgctgca
     1801 acggtcaatt gcctgacgaa tatcaccgcg atcaatcatc ggtaaagcgc cacgttcttt
     1861 aatctgttgc agtgccacag cgtcctggct tttcggagag aagtctttca ggccaagctg
     1921 cttacggtaa gcatcccacc aacgggaaag aagctgataa cgtccggcgg ctgttgattt
     1981 tagttttggg tttagtgtga caagtttgcg aggatgatcg gagtaatcag taaatagctc
     2041 tccgcctaca atgacgtcat aaccatgatt tctggttttc tgacgtccgt tatcagttcc
     2101 ctctgaccac gccagcatat cgaggaacgc cttacgttga ttattgattt ccaccatctt
     2161 ctactccggc ttttttagca gcgaagcgtt tgataagcga accaatcgag tcagtaccga
     2221 tgtagccgat gaacacgctc gttatataag cgagattgct acttagtccg gcgaagtcga
     2281 gaaggtcacg aatgaaccag gcgatgatgg cgcacatcgt tgcgtcgatt actgtttttg
     2341 taaacgcacc gccattatat ctgccgcgaa ggtacgccat tgcaaacgca aggattgccc
     2401 cgatgccttg ttcctttgcc gcgagaatgg cggctaacag gtcatgtttt tctggcatct
     2461 tcatgtctta cccccaataa ggggatttgc tctatttaat taggaataag gtcgattact
     2521 gatagaacaa atccaggcta ctgtgtttag taatcagatt tgttcgtgac cgatatgcac
     2581 gggcaaaacg gcattaggtt gttagcgcaa cctcatgccg cccactttca cgaagatcat
     2641 gtgtagaagg ccgcagcgta actatcactg atgaattcag gatagtcagt ggctacggct
     2701 cagtttggat tgtggcgacc ggtgctgatc tccggtttgc tgcaactgcc tacagctggc
     2761 tacgtggcca caccgaatcc agcgaaagat tcttgccctt acacatcagc ctgtgcattc
     2821 accacaacgg aaagagcact ggctaaccag gctcgccgac tcttcacgat tatcggctca
     2881 atgctcttac ctgttgtgca gatataaaaa atcccgaaac cgttatgcag gctctaacta
     2941 ttacctgcga actgtttcgg gattgcattt tgcagacctc tcagcctgcg atggttggag
     3001 ttccagacga tacgtcgaag tgaccaacta ggcggaatcg gtagtaagcg ccgcctcttt
     3061 tcatctcact accacaacga gcgaattaac ccatcgttgg gtcaaattta cccaacttta
     3121 ttcaataagt caatatcatg ccgttaatat gttgccatcc gtggcaatca tgctgctaac
     3181 gtgtgaccgc attcaaaata ttgtctgcga ttgactcttc cttgtggcat tgcaccacca
     3241 gagcgtcata cagcggctta acagtgcgtg accatgtggg ttgggtaagg ttggggatta
     3301 gcatcgttac agcgcgatat gcggcgcttg ctggcatcct tgaatagccg acgcctttgc
     3361 atcttccaca ctctttctca acaactctcc cccacagctc tgttttggca atatcaaccg
     3421 cacggcctgt accgtggcaa tctctgcatc ttgcgcccgg cgtcgcggca ctacggcaat
     3481 aatccgcata agcgaatgtt gcgagcactt gcagtacctt tgccttagta tttccttcaa
     3541 gctttgcaac gccacggtat ttccccgata ccttgtgtgc aaattgcatc agatagttga
     3601 tagccttttg tttgtcgttc tggctgagtt cgtgcttacc acagaatgca gccattccga
     3661 atccggcttg tgattgcgcc atccccatag cagccatcac atcagtaccg gaaagagagt
     3721 cagaagccgt ggcccgtggt gagtcgctca tcatcgggct ttttggcgaa tgaaatttag
     3781 ctacgctttc gagtctcatc gtcttcccct cttgccctgt ttgaccatca ggacgccgtt
     3841 aactattacg tgacgctcgc ctttgctgtc tcggttgtac ttgagcactg ttcctcttgc
     3901 gcaggaaagc atcctcgcca cttcggtctg attgcctcgt gtctggataa gaagctctgg
     3961 tatcgtttga attgtggcgt tcatacgttc tccagttcgg tgatttttat tccaagcctt
     4021 ccgcctggta ctttcacacc acgaattacg cgaatgtcat cgaattgctc gtcgtcttcc
     4081 gcaaatccgg cgtggataag ggagtcgagt aaacctttca ggatgttatc gaggtcgcgg
     4141 cggcgggagt ctggaacgtc tgcgatgact ttgatgcgga gtcgtgattt ggtgaaaatg
     4201 tctaacttga gttggaggat tatttgctga acttcttttc ggtatttctg gcctttatcg
     4261 ctgatgtagt attggtttcc ccgtcttcgc cagtaggtgt tcaccgacgg cgggtatgga
     4321 agcacaaact gatattcgct catgacttaa tcttcccctc cttcagcagt atcgcctgcg
     4381 tcctgatcac gccttcgagg tggtaaagtc tggcgtcttt gttgtcgaga ttatgggtgc
     4441 gtcggtcgat ttcatcgtga cacgcgctac aagcccatgc gccgatcagg tcgtcaggct
     4501 tcattcccgt tccgcaaatt ccagccatcc ggtaatgtgc cagaactgta gtttcaggat
     4561 taccattgca tacgccgtaa atacgtacct ggcattctct gccgcgtgct tctttgcgta
     4621 gattagccat taagcagcct cccctgttac tttcagcatt ccattatcga gcagctttct
     4681 ggtcagccac tgttgaccac gcccggtgat ttttgtggtg aacgatatct gtattccgtg
     4741 atttgtgttg accgctgttt ctttcactgt gaaatagccg cgatccatat attcctgcat
     4801 tggcacattg cgccgggaac ctgaagcaat aaggattttg tgatcgcgca tccacgcaaa
     4861 cagtttgttt ggaccaattc caacaacctt tgcaaagttt ccaatcaaaa ttccgctggc
     4921 ctcgccaacg cgatcggcaa actcaacttt aggtgcggca attgcgagct ggttttccag
     4981 ttgcattttc tgctcagcaa gatcagcagc aaggcgcaac gcttccggta gcgttttggg
     5041 gatattaacc gcagcttctt caagctctcg ccagcggtca acaaggcgag cggtgaattc
     5101 cggcgacaac tgggctacaa cgacaatact gtctcgcttt ccttgttcgc cttcgaacac
     5161 atacacacaa aaactttgat ttaagcctaa cccattgatt cttccacaat cctcaatttg
     5221 aggaagccgg ataacaccat ttttagccag cgtttcgatg gtacgtttca cattgtcatg
     5281 acgcttacca accaactcag cgatttcaat gcttgtcatt ttgatggcat tgccatttat
     5341 taactcattc atcgtcttct tcctcgtaca ttgagctatt cggatcgctc atcagttctg
     5401 cgcagcagtg ctcacacacg tgaacttcca gcacatgcag cttctgaccg caattagcgc
     5461 acgttaaagc tcgctcgacg ctttctttct ggtattgaag ggattgggat gggctaagca
     5521 tggctttcac cattaaaaag tcgcttgtaa gcatcgatgt ctcgttttgc ttcaccgagc
     5581 tttcgtctta attccatgtt ttctgattca agcttttcca tgtcttgttg gtatcgatcg
     5641 cggtgttctt tccatgcttt tcgatacgcc ttcatgtatg tcgtattggc ctttctcttt
     5701 gcctgacgaa ctgcgtggtg gtttttcaca aaccagtcag ggtcgttaaa tgctgctctg
     5761 gcgcaggtat accaataatt tgttgcctcc ctgtttagcc aataaatact gataaatggc
     5821 aactgtatcg acaccatttt tcgttgagac tctttctcgc caaacatgtg cccttttttg
     5881 atgctaaggc caaatccagg ttgaattaaa agcattgtca tttcctcgca cgatgaaccg
     5941 ccccgggaat cctggagact aaacttcctg agaa
//
LOCUS       DADMGC010000187         5954 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.187, whole genome shotgun sequence.
ACCESSION   DADMGC010000187 DADMGC010000000
VERSION     DADMGC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.187"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..1110
                     /locus_tag="JNZ73_003850"
     CDS             <1..1110
                     /locus_tag="JNZ73_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HAY8733956.1"
                     /translation="WPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMA
                     VAIQHGSFIEDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPCEPLHIVTDQ
                     ALNAEIQKGEEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRD
                     PFDWAEKNLQEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVF
                     IDNYAAIPSESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFID
                     ARGQRPLKVKDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHD
                     QPFVQGLTACAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(1122..1934)
                     /locus_tag="JNZ73_003851"
     CDS             complement(1122..1934)
                     /locus_tag="JNZ73_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733957.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(1938..2723)
                     /locus_tag="JNZ73_003852"
                     /pseudo
     CDS             complement(1938..2723)
                     /locus_tag="JNZ73_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
     gene            complement(2720..3388)
                     /locus_tag="JNZ73_003853"
     CDS             complement(2720..3388)
                     /locus_tag="JNZ73_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY8733958.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(3452..4090)
                     /locus_tag="JNZ73_003854"
                     /pseudo
     CDS             complement(3452..4090)
                     /locus_tag="JNZ73_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
     gene            complement(4103..5954)
                     /locus_tag="JNZ73_003855"
                     /pseudo
     CDS             complement(4103..5954)
                     /locus_tag="JNZ73_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
BASE COUNT         1511 a         1597 c         1369 g         1477 t
ORIGIN      
        1 tggcctgatg aagaagaagc aaccagaacg tattttccca gcccgtggtc aggcctgatg
       61 gaagcaaagg tcgatgcgtg taacgtgggt attatgggaa catccttgag cggactggat
      121 gcggcaatgg cagtggctat tcagcatggt tcgttcattg aagatgataa acaacacgtc
      181 gtttttcacc gcgataacgc aagtgaaaag ctaaatatta cgttgatgtc gcgcacgggt
      241 attttacccg aagccgattt ctattgccct attccctgcg agcccttaca catcgtcacc
      301 gatcaggcat taaatgctga gattcaaaaa ggtgaagagg gccttttgga tcgggtattt
      361 agattgatag tagaggaaat caagtttgct gatccagact ggagccaacg catagcctta
      421 gagagcctga atgtcgattc ctttgctcaa gcctggtttg ccgagcgcaa acaacgcgac
      481 ccatttgact gggcagaaaa aaatctccag gaagtcgaac gcaataaacg agaaaaacat
      541 actgttccct ggcgttatgt cattctgcgc ctgcatgaag ccgtacagga aattgttcct
      601 catctgaatg aacacgacca taaacggttc agtaaaggcc ttgccagggt tttcatcgat
      661 aattatgcgg caatcccttc agagtctatt cgtcgactac tggccttacg tgaagcgggg
      721 atcattcata ttctcgccct cggtgaagac tacgaaatgg aaattaatga gtcgcgcacc
      781 gtcctgaaaa cggaagacaa cagctactcg tttgacgttt ttattgatgc ccgcggacag
      841 cgtccgctta aagtgaaaga tattcctttc cctgggctac gcgagcaatt acagaaaaca
      901 ggggatgaaa tccctgatgt tggcgaagat tatacgttac agcaacccga agatattcgt
      961 ggacgcgtag cgttcggcgc gttgccctgg ctgatgcacg accagccttt cgttcaggga
     1021 cttacggcat gtgcagaaat tggtgaggcg atggctcggg cggtcgtaaa gcctgcatcc
     1081 cgtgctcgtc ggcgtctttc gtttgattaa aggtaaagct atcagcactc aacaaactga
     1141 tgctgttcat taagacgata gcggactccg gttcgaatat tgttttcacc ttcaatggcg
     1201 acggcgaagc gaactctttc gccgtcatga tatgccagtg cggcgcttcc ccctagcccg
     1261 agaataattg agtcgaccac gcccgtgctc actaccgttg aattttcacc gctggcaatg
     1321 atgtgtacat tgtcgccgct gttggcaatt ctggcgtttg cgccaaagct ggcgatttgc
     1381 accaggtcac cattactggc gacatgacac cgttcaccca atgtacaaac gcgtactcgc
     1441 attccggtat tcgcaatccg actactatct ccagcgctgc ttatccgcga agaattaccg
     1501 gttacagcaa cccgtgcgcg ttcaccggag ctgccaatgt gactgttaaa gccgacactt
     1561 ccaatccgca cgctataacc catactggca atttgcgcgg catacccagc ggttgcgatt
     1621 cgggcgttat cctcacaaca gccgatgcga gcatgatcgt cgctgcgggc aaactgactg
     1681 caaaatggta tctctccggg gtctgtggcc agtttttgca tagaggaaac ttcctgatgc
     1741 gcgaagtttt catccgccag ccattttttc cacgcatact caaccagact ctccgcccag
     1801 tccgtatatc cttcctcaat cagtgcatga cgaatatcgg cataactacc gccgcgtggg
     1861 aaatggcgca ggaaccagcg atacatcacc gctccgatac gccattctcg caggtctgct
     1921 cgtgtaatga tcattcgtta gtgccggtga tagccatcga ccatgctttt aaaggtttcg
     1981 tgtggcgtac gccccgtggt gcaaagataa agatgaccga agataaaaaa gaggcttata
     2041 aatgccagag caaaatgcgc ctgcaataac cagtatctta cgccaggaaa cacatctccc
     2101 acggcttgcg gatagagaca caacagcccc gtcaatagta acaacggcag caatccatac
     2161 atgacaccaa cataggcgac ctgctgtaag ggattaaatt tagactgtgt tgttgccggg
     2221 aaaggatgtt cttccccctg cataatgcca aacaaataaa atcgcgtttg ttttgccgct
     2281 cgttccagcc acccctgacg acgaatgcga tagtggtgac cattatcccc aacggcattg
     2341 atcagcacaa agccgagcca gcatgccagt aacaaaaatc agcaaacttc atgcaccgca
     2401 accagacttt taaccgcagt tgcgcccacc atcgcaaagt gatttatcag cccacttgcc
     2461 agcaacaata caaagagtaa cgcattcgac cagtgccata atctgaccgc tttgctgtaa
     2521 agataaacct tctcaccatg atcggtggct gatttcttca cgccacgagc acgaagcaag
     2581 gcgtgcagcc ccagcaccaa ccacatccca accagcaata ctccggcaat gatcaaccac
     2641 accggccaaa attcgggagt gaactgtggc acatagttcg ctaactggct ctgaaactgt
     2701 tcagcatgtt gcgacgggtt catacatttt cctttttaat caaatgttga ccgaaccggc
     2761 gatacagatg cggttttccg gcaccaggta gctgatattg atagtattta ttatcctgca
     2821 accacgcctg aatttctggg ctatcttcgc gaccaaagat caatgcgtgt tccggacagg
     2881 cgctgacgca aatgggcgga aagccttttg ccagtcggga ctcagcgcag aaatcacatt
     2941 tatctgccac tttagtgaca gggttaaggt agcgaacctg gtacggacaa gcgccaatgc
     3001 aataactgca gccgatgcac tgcgattttt ctacccgcac gatcccctgc tcatcacgcc
     3061 acgatgcccc cgtcgggcat acgtcgatgc acggagcatc ttcgcagtgc tgacatgact
     3121 ggcgaaaaaa gtgatactgc gtttcgttgt cgttatcggt gactggaatg tgtgctatcg
     3181 ataaacggct tccctgagcg gggacgtggt tagttttacg gcatgcgcgg gcgcaaatat
     3241 tacagccatt gcaccgtgac tcgtcatgaa tcatggcata ccgaacttcc ttttcttgcc
     3301 tcgtgttcgc taacagagaa gacgcagaac cagtaaaaaa gattactgtt cccatcccca
     3361 gaacaaattt gcgtcgagtg aaggacatct gattatcccc taatgcagtg gctgcaactg
     3421 tccaccctat ccatgacatc tccctgaaat ttcaggggtt ctgaaactgc tctacctcga
     3481 accattcgca taagccataa gccttgccat aggtcataaa tcgctcgcta aacaagtaca
     3541 acaccgccgt cgatgactcc acctgccgta tatcggcata ttcaggtgca acatccatcg
     3601 cggcggtcgc ggcttcaatc tgcgcttcct agaagtaata tggcgcttgc atcagcatgg
     3661 cgactttgta aggacgagga tacgtctggc actcaaaacg aacggcgtgt gcaatggcgc
     3721 gacaaatgtc ctgctccaca acttgcaaag acatcgccgc gtagttttca ctcatggcct
     3781 gcgttgagta atagtaatca tcctgacttc ccgtcataca agcgatatcc gcgtagtctc
     3841 cgttctgctt aatgctttcc agcaaaactg acagttcctc ttctgcaacc gaatagggtg
     3901 gcgagagaaa cacggtgcgc gccaccagct gcccagccgc agaatgctgg cgaatgaact
     3961 cagccaggat ttcagcaggt tctggttcgg ttaccgtatc aacggggtga cgcatcccct
     4021 gcaaccattg ccgcttcgcc tcgtcaactt cgctgacttt cgctaacggc aattcatcct
     4081 gatggttcat cttacccatt ccttacgcag gcagtagatt gtgtgctgcc agatcggcgg
     4141 caatatcttc cagccccaga cgctgcaatg tttcgcgggt tggacaacca agctgtgggt
     4201 cccagcccat ttctttgtag aacatcgtga gcgaagcatg catatcgtca cgatccattt
     4261 tgtcagtacc ttcagtaaag accgggatct gcggatcctt gtcgaaaacc caggaacaga
     4321 taagatcgtg ttcattacgc atatctttgg tttgcatcgg ttttaccgtg taggcacgat
     4381 gcaacgtaaa gatacgctct gcagctaaat ctaatttttc ctgagtcatc tcttcgccgg
     4441 tgatcgcttt gaagaatttg gcttcaagcg ccagatcgcc ccggtaatta cggcttttca
     4501 gcggcgaaac ggtcattggc cagacccagt tgcacagggt gacggcgtta tgcaaacaga
     4561 cccgcaacag cgaccacttt gcatatttaa tttttgcgtc attgattggc gtgtagtttt
     4621 tggtttcatc gtaagcatct tcagaaccaa aaagttcttt cgccacttca cgttgcagtt
     4681 tcaatggcaa gccggaacca ataaagttga tatgggtatg cgtcatgcag tcacggttga
     4741 acatacagtt aacaatgaaa ccgacctgcg ctgacgcttc attggcatgg tgaaccggat
     4801 agccaaacgg cgaccagagt ttatttttcg cgtagcccca gtactcttta cccaaattcc
     4861 agcgttctgc gatggcatat gaaccataag ccaggtgact cagctcaccc acacgatgcg
     4921 ccagacggta gtaaaaatct ttaatgaagt taacgtcacc cgcttccagt tgatcccagc
     4981 gaatttctgc atactcttca gctggcagaa cacgcttgaa cacacctttg ctgtaacaat
     5041 aagtaaagtc gcgatgcaac tgcccgtagt tacaccatag gccgtagtca tcgaacaaat
     5101 tcagaccaac caggttacca atcacacgac catcgtcttt atcttcaaaa tctttcggcc
     5161 cgttcgggaa gatggtggta tggacaaagt ttgctacaca agtgttacca cctgtgctgg
     5221 gcacgccaaa ctctttcacc cgaggaatat tcatttgggt catacaacgg atcgggcaag
     5281 agtggcagcc gctcattttt actgtgtatt tctctgccgc cggtcctaag tcaaaaacgg
     5341 atttataggt acgaaagccg accgtatttt gattgcctgg cggaatttca cccgtttcaa
     5401 tcggaccacc ttcagccgcg ccccaaaaca gccctttacg tgctgtccag cgtgacttgg
     5461 gatctgaata ctctgcccac gattgtggcg tacttggcac gacatggttg ttattcgcac
     5521 caataagttc agtcatcatg taatcattga gacgcttcat ctcctgacga tcggcaatgt
     5581 tgaccccttt cgtcccttca accgcaatcg ctttcaggtt tttcgaaccc attattgcgc
     5641 cagttcccgc accgccgctg tggttacggc tattcaacat gccagagaga ggaataaggt
     5701 tttccccagc ctgaccaata gccgccacac aggtttcccg actggtcaat cgacaaattt
     5761 cttccgtcgt tgcgcgcgtc ccttttcccc ataagaaatc ggctttttcc aggctaactt
     5821 tgtcatcttt aatcttcagc cataccggtg atttcgcctt cccttcgata ataatgacgt
     5881 cgtatccagc gaatttcatt tgcgctgcaa aaaagccacc catatgggca tcgcgactaa
     5941 atttcctttg gtaa
//
LOCUS       DADMGC010000188         5798 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.188, whole genome shotgun sequence.
ACCESSION   DADMGC010000188 DADMGC010000000
VERSION     DADMGC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.188"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            51..467
                     /gene="torD"
                     /locus_tag="JNZ73_003856"
                     /pseudo
     CDS             51..467
                     /gene="torD"
                     /locus_tag="JNZ73_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
     gene            complement(560..865)
                     /gene="cbpM"
                     /locus_tag="JNZ73_003857"
     CDS             complement(560..865)
                     /gene="cbpM"
                     /locus_tag="JNZ73_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HAY8733959.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(865..1785)
                     /gene="cbpA"
                     /locus_tag="JNZ73_003858"
     CDS             complement(865..1785)
                     /gene="cbpA"
                     /locus_tag="JNZ73_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HAY8733960.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFFEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            2046..3302
                     /locus_tag="JNZ73_003859"
     CDS             2046..3302
                     /locus_tag="JNZ73_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HAY8733961.1"
                     /translation="MGSNIHGMSCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPRISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            3595..4836
                     /gene="agp"
                     /locus_tag="JNZ73_003860"
     CDS             3595..4836
                     /gene="agp"
                     /locus_tag="JNZ73_003860"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HAY8733962.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(4874..5101)
                     /locus_tag="JNZ73_003861"
     CDS             complement(4874..5101)
                     /locus_tag="JNZ73_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733963.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(5122..5718)
                     /gene="wrbA"
                     /locus_tag="JNZ73_003862"
     CDS             complement(5122..5718)
                     /gene="wrbA"
                     /locus_tag="JNZ73_003862"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY8733964.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
BASE COUNT         1547 a         1316 c         1395 g         1540 t
ORIGIN      
        1 accttaaaat caccatcata cactaaatca gtaagttggc agcattacct gccacgctga
       61 cagtacgtga cgatgcccgt ctggaactgg ccgctgactt ttgcggcctg tttctgatga
      121 ccgacaaaca agcggcgctg ccgtatgcat cggcctacaa acaggacgag caagagatta
      181 aacgcttgtt agttgaggca gggatggaaa ccagtggcaa tttcaacgaa ccggcagatc
      241 atctggcgat ctatctcgag ttgttgagtt atctgcattt ttcgctggga gaggggaccg
      301 ttcctgcgcg aagaatcgac agtttgcggc aaaaaatact gacggcgcta cggcaatggt
      361 taccagagtt tgctgcgcgt tgtcgacagt atgacagctt tggtttttac gcggcactaa
      421 gccagttatt gctggtgtta gtggagtgcg accaccaaaa cagataacgt cgtttatgcg
      481 cctgaaaaga cgcgtttagc gtcgcatcag gcattatggc gcagttgccg gatgcgtcat
      541 cacatccggc aaacacacat cacggatgag ctacaaaccg ggaaagccgc tggcgtagca
      601 ggcggttttc ctgcttcagg tgcgcaatat catccattaa cgtcagcgcc accgcgatcc
      661 ccggccagtc cagagccagt tcatgacgca ggcgtaccgc gcgttgcacc acaatggcgg
      721 catggtcgtc aaatacccag gttgtttcct ggatctcacg cggttcaacc acccccaaac
      781 cgacaatttc attcaactcc tcttcagaga tgccggtatg caggcaaaat tcggtaatag
      841 taaaagtcac cgtaacatta gccattatgc tttcccccaa tctttacgtg gatcaaaaga
      901 cgactgggcg tctgccagtt gctgccacag cgcggcagtg ttttcatccg gtttcggcgg
      961 catcacgatt ttcagtaccg catacagatc gccggtctgt tttttgctca ccagaccttt
     1021 gcctttaacg cgcaatcgtt gcccggcctg gctgcctggc gggatagtca gcaaaatgct
     1081 ttctttcagt gttggaacgg tgactttagt acccagcgcc gcttcccacg ggctaaccgg
     1141 caccacaatt tccagatcct ggccgacaat atcgaacagc ggatgtggcg caatatgaat
     1201 caccagccac aaatcgccat ttggaccgcc gttttcgccc ggcgtcccct ggcctttcag
     1261 gcggatgcgt tgaccattgc cgacgcctgc cgggatcttc acattcagcg ttttcgggat
     1321 ttcctgttcg atcatgccaa aggcgttata aaccggcagg ttatagctga tggtacgctt
     1381 atgctcagta agcgtttctt cgaagaatac cgccacttcg atttcaatat cgtggccgcg
     1441 tgcggcgggg cgttgacggc tctggcgggc atgctgaccg aaaattgacg agaagatatc
     1501 gtcaaaatct tcggcattaa aactctgacc gtcgccatga tggaactgac ggttaaattg
     1561 cggatcgttg cgatgttgcc acatctgatc atactcagcg cgacgttgtt catcacttaa
     1621 cacttcccag gcttcagcga cctctttgaa gcgggcttcg gcatccggtt ctttgctgac
     1681 atcaggatgg tatttacggg ccagtcgacg ataggcggtc ttgattgtct tgagatcgtc
     1741 cgtcggtttc acgcccatga tggcgtaata atcctttaat tccatagcgt tatctcgcgt
     1801 aaatcaacac aaattgaagg aacccctgta aggtaactcc tataagtgta gggtaatcct
     1861 caaaatttca tatgccaaca cagaatatgt tattgaaatc atcgcggaga ggaggtcgcc
     1921 atcaagatgg gttgctgaac atattttaaa caggtgaaaa agggtgagcg atttttgata
     1981 gttgaaccag gcactttaag tttaactagg gcgtcattat ttattaaatt ttatagacgc
     2041 tatatatggg tagtaatata catggaatga gttgcactgc aaataattat ttgaaacagg
     2101 cctggaacga tataaaaaat gagtacgaaa aaaatcaaac atattcaatc acgctttttg
     2161 aaaacacact ggtgtgtttt atgcggttgt acaatgaact cagacgtaaa gtaaatgaag
     2221 aggatactcc atgtctggaa tgtgaatcac tagaaaaaga atttgaggaa atgcagaatg
     2281 ataatgatct atcattattt atgagaacat tgcgtactaa tgatacacaa atttattcag
     2341 gggtttcagg aggtattaca tatactatac aatatgttca agatgttgat attgttagag
     2401 tgtccttgcc gggcagaggt tcagagtcta tcacagattt taaaggttat tattggtatg
     2461 gctttatgga atatatcgaa aacattaatg cgtgtgatga tgttttttct gagtattgtt
     2521 tagatgatga aaatatgagt atccagccag aacagataaa tatgccgaga atatctgatt
     2581 tagatactgg tattgatttg tctggcatat cttttattca gagtgaaatt aataaaacat
     2641 atggattaaa atacgctcca gtagatggcg atggatattg tctgttaaga gccatactgg
     2701 ttttaaaaga acatgagtat tcatgggcgc tgggcagcca taagacgcaa aagcaagtat
     2761 acgaagagtt tattaaaatt gttgataaac aaacgatcga ggctcttgtt gatacggcat
     2821 tctataatct cagggaagat gtaaagacat tatttggcgt tgatctgcaa tctgacaaca
     2881 aaattcaggg tgtgggtagt tttatgtcat ggagctttct gttttttaaa aaacaattca
     2941 ttgatagttg cttgaatgac aaaaaatgca tactgcattt acccgagttt atatttaatg
     3001 ataacaagaa tttgcttgct ttagataccg acacgtcgga taggattaaa gcggtgaaga
     3061 attttcttgc tgttctttca gatagcattt gctcattatt tattgttaat agtaatgtgg
     3121 catcaatctc cttgggaaat gaatcctttt caacagatga agatcttgag tatggttatt
     3181 taataaacac tggcaatcat tatgatgttt acctccctcc tgaacttttt tctcaggctt
     3241 ataagttaaa aaataaggaa atgaatgcgc aacttgacta tttaaatcgt tatgcaactt
     3301 aatggcaaag gcatatgcta aaaaccattg ttattagtct cacacttttt tattggtaaa
     3361 tattgtctct gtattggtaa cgccgcagat attctgttta gccacaggtg caattatcag
     3421 cggcgtacgc gaggcagggg ctaatcaggc atagtttgcg tcaaaccttg cctgtttttg
     3481 aagatgtata tagaaaaaca ggcgttcaac aagccatttt gcgaacctgt tcccggaaaa
     3541 aagtcatatt tctgtcacac tctttaatga ttgataacaa aagaggtgcc aggaatgaac
     3601 aaaacgctaa tcgccgcaac tgtggcaggg atagttttac tcgcttcaaa tgctcaggca
     3661 caaaccgtac cggaaggcta tcagctacag caagtgctca tgatgagccg ccataactta
     3721 cgtgcgccgc tggcgaacaa tggcagtgtg ctggagcagt cgacgccgaa taaatggccc
     3781 gaatgggacg tccccggtgg gcaactcacc accaaaggtg gcgtgctcga agtgtatatg
     3841 ggccattaca tgcgtgaatg gctggcagag caggggatag tgaaatcggg ggaatgcccg
     3901 ccgccggaca ccgtttatgc ctatgccaat agtctgcaac gtaccgtcgc taccgcccag
     3961 ttctttatta ctggtgcatt ccctgggtgt gatattcctg tgcatcacca ggaaaaaatg
     4021 ggcactatgg acccaacctt taacccgatg atcaccgatg attccgccgc attcagtgaa
     4081 caggcggtgg cggcaatgga gaaagagctc agcaaactcc agcttaccga cagctaccag
     4141 ctactggaaa aaatcgttaa ctataaagat tcccctgcct gtaaagagaa acaacagtgt
     4201 tcgctggtgg atggcaaaaa tacctttagc gccaagtatc aacaagaacc aggtgtttcc
     4261 gggccgctga aagtcggcaa ctcgctggta gatgcgttta ctttgcaata ctacgaaggt
     4321 tttccgatgg atcaggtggc ctggggagaa atcaaatctg accagcagtg gaaggtattg
     4381 tcgaagctga aaaacggtta ccaggacagt ctgtttacct caccggaagt ggcgcgcaat
     4441 gttgcgaaac cgctggtcag ttatatcgac aaagccctgg tcaccgatcg cgccagtgct
     4501 ccgaaaatca cggtgttagt tgggcacgac tccaacatag cctctctgtt aacggcgctg
     4561 gatttcaaac cgtatcagtt gcatgaccag aacgaacgca cgccgattgg cggcaaaatc
     4621 gttttccagc gttggcatga cagcaaagcc aatcgcgatt tgatgaaaat tgaatatgtg
     4681 taccagagtg cggaacagtt acgtaatgcc gatgcgttaa ccctgcaggc acctgcgcag
     4741 cgcgtgacgc tggaattaag cggttgcccg atagatgcca acggtttctg cccgatggat
     4801 aagtttgata gcgtgttgaa tgaggcggtg aaataacaga aaactccccc gcgagaagcg
     4861 ggggagtcgc tggttaaacg tttttacgtt cgatggtctg ttcgccccaa aaaagcgaat
     4921 ctttatcggt cttagcaaag gctttgacta acacttcatc actaccttct tcccaaatct
     4981 tttctgccat tttttcgtcg tacccggcga cttcgaaaat ggcctcggct atttccggcg
     5041 acgtattgcg cagagatgcc cattcaccga cgtgatgagc tttcgcttct tgagttggca
     5101 tgcgtatcct cctgttgaag attagccgtt aagtttaact gccagacctg cgacatattc
     5161 cccttgataa cgagcaatag acagttcttc ctggcttggc tggcgtgagc cgtcaccgcc
     5221 tgcgatggtg gttgcgccgt acggcgtacc gccgcgaacc tgtgaaacgt caaataattc
     5281 ctgcgctgcg tagccaatgg ggacaatcac catgccgtga tgcgcaaggg tcgtccaggt
     5341 ggatgtgatg gtttgttcct gaccgccgcc agtaccggtg gaactaaaga cgctcgccag
     5401 ttttccgtat agtgcgccgg aagcccacag gccgcccgtc tggtcgagga aggtacgcat
     5461 ttgaccggac atgttgccaa agcgggtagg tgtaccaaaa ataatggcgt cgtaatcggc
     5521 cagttcttgc ggggttgcaa ccggtgcagt ttgcgtttta ccgcctgctt tttcaaataa
     5581 ttgcggcggc atggtttccg gtacacgctt aacgacaact tctgcaccat ccactttgct
     5641 tgcaccctca gcgactgcgc gtgccatcgt ttcaatatgt ccgtacatgg aataataaag
     5701 caccagaact ttagccattt ctaaccactc ctcgtgttat ctctattccg tagcgattcg
     5761 ctaccactta tttaaagata agacgtcctt ttcagagt
//
LOCUS       DADMGC010000189         5786 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.189, whole genome shotgun sequence.
ACCESSION   DADMGC010000189 DADMGC010000000
VERSION     DADMGC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.189"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..789)
                     /locus_tag="JNZ73_003863"
     CDS             complement(<1..789)
                     /locus_tag="JNZ73_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M15"
                     /protein_id="HAY8733965.1"
                     /translation="MTQYSSLLRALAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQVLKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLG"
     gene            1035..1661
                     /locus_tag="JNZ73_003864"
                     /pseudo
     CDS             1035..1661
                     /locus_tag="JNZ73_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            complement(1658..1864)
                     /locus_tag="JNZ73_003865"
     CDS             complement(1658..1864)
                     /locus_tag="JNZ73_003865"
                     /inference="COORDINATES: protein motif:HMM:NF019607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY8733966.1"
                     /translation="MAAKIIYWALKDKDVIVMSVNGDKVTEDGRILTDRGQRIRDVRT
                     GPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(1834..2772)
                     /locus_tag="JNZ73_003866"
     CDS             complement(1834..2772)
                     /locus_tag="JNZ73_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY8733967.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARGEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGR"
     gene            complement(2883..3266)
                     /gene="bssR"
                     /locus_tag="JNZ73_003867"
     CDS             complement(2883..3266)
                     /gene="bssR"
                     /locus_tag="JNZ73_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HAY8733968.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3479..4804
                     /gene="rimO"
                     /locus_tag="JNZ73_003868"
     CDS             3479..4804
                     /gene="rimO"
                     /locus_tag="JNZ73_003868"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAY8733969.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNIKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4851..>5786)
                     /locus_tag="JNZ73_003869"
     CDS             complement(4851..>5786)
                     /locus_tag="JNZ73_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY8733970.1"
                     /translation="FISFDGKTFVYYLYPVTHNRSLIFLLGLERFSLLSKSLAMDSEN
                     LMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVFAYKKDVYLRVCTLII
                     FFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDGGLFFSAADVKKLYSM
                     YNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNINDTFGHLLGDEVLMK
                     VVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEIVHFQVGSINLSTSIG
                     VAHSNKCTTVERLKMLADERLYKSKKNGRAQISWQ"
BASE COUNT         1482 a         1488 c         1372 g         1444 t
ORIGIN      
        1 ccccagcact acggagatta aacgcatatc accctgggta gccgaagcaa ccagattata
       61 tcccgcacct gccgtggttc ctgtcttcat gccatcaaca ttcagattgc tgctccacag
      121 cagacggtta cggttaggct gacgaatttt gttgaaggtg aactctttct ctttatgaat
      181 ggcgtactct tccggcacat cgtggatcaa tgctttaccc agcaatgcca tatcgcgcgc
      241 ggtactgaac tgccccggcg catccaggcc gtgcaccgtc tggaaggtag tgttggtcag
      301 ccccagtttt ttggcataac cattcatcag accaataaag gactcctggc tcccggcaac
      361 gtaatcggcc agcgcaatac aggcgtcatt accggactgg ataatcacac ctttgttcaa
      421 gtctgccacc gaaacctgat cgcccggttt gaggaacatt accgatgaac cacgcagtgc
      481 cggatttccc gtcgcccagg catctttacc gaccgtcacc atatcggtga gtttaatctt
      541 atcggcctta agcacctgcc caaccacata gctggtcatg attttagtca ggctcgcggg
      601 atccagtttc tcatccgcat tgccttctgc cagcacttta ccgctggcgt aatccattaa
      661 aatccatgca cgcgcatcca cgctcggcgc ttcaacggtt tgttcggccg cgaatgccgt
      721 tggggcaaaa aggaataaaa atgcagaacc cgctgcaaga gcacgaagga gagaggagta
      781 ttgcgtcata aatgccaccc gagtatccat tcaaaaaaat acgttatatc gccgtatgat
      841 aagttcggtg attaataacg cacacacaaa cttaaagaaa ccgtaagttg gtaaagtttt
      901 taaagtttat gcaatatcct gccgcttcgc tgcttctggt gtcatttttt tgccagctgt
      961 tgcttaaaag ttaactttac ttcaccatcg gagtcctgtt cagtctgtgg ctgcctgtcg
     1021 gaaaaggggt gagtatgatt acgctgtggg gtcggaataa ttcaactaac gtaaaaaaag
     1081 tattgctgac gctcgaataa ctggaactac cttatgagca aattctcgcg ggccgtgagt
     1141 ttgggataaa tcacgatgct gattttctgg cgatgaaccc taacgggctg gtgccgttgt
     1201 tgcgtgacga cgaaagtgat ctcattcttt gggaatcaaa cgccattgtc cgctatctgg
     1261 cggcacagta cgggcaaaaa cgcctgtgga tcgactcacc ggcacgtcgt gcggaagcgg
     1321 aaaaatggat ggactgggca aaccagacgc tcagtaatgc tcatcgcggg atcctgatgg
     1381 gattagtcag aacaccaccg gaagagcgcg atcaggccgc cattgatgcc agttgcaaag
     1441 agtgcgacgc cctgtttgcc ctgctcgatg cggaactggc aaaagtaaaa tggttctctg
     1501 gcgacgagtt tggtgtgggc gatatcgcta tcgcaccgtt catctacaat ttgtttaacg
     1561 ttggcctgac ctggacaccg cgtccaaatc tgcaacgctg gtatcagcaa ctcactgaac
     1621 gccccgcagt gcgcaaagtg gtgatgattc ccgttagcta attgcgtggg ctaactttaa
     1681 gtaattcccc actggactcg tcggtgagaa cgtataaata accgtcgggt ccagtgcgaa
     1741 catcacgaat tcgctgccct ctgtccgtta aaatacggcc atcttctgtc actttgtcgc
     1801 cgttgacgct catcacaatg acatctttat ctttcagcgc ccaataaata atttttgctg
     1861 ccactggggg aatttatcgc tgttatagaa ggccatgccg ctcacagcgg gcgaatcttt
     1921 ccagtaaaaa acaggttgct cggtcccggc gacgatctcc cctttcgctt ccggtatctt
     1981 aaagcctgaa tagttgattc cccaggttgc cagcggccag ccgtagtttt tgcctttttg
     2041 cgggatatta atttcatcac caccgcgcgg gccatgttca ttcagccaca gtgcattact
     2101 ccacggattc atcgccattc cttgcggatt acgaatgcca taagaccaga tctcgccgcg
     2161 cgcaccggat tcctttataa aaggattatc atccgggatt tcgccctggt cggtcagacg
     2221 caccagtttg ccctgtaatt tatccagatc ctgcgccgtc gggcgctgat tgttttcgcc
     2281 cagagcaata aaaagataac ctttaccgtc gaataccagc cgcccgccaa aatggttgcc
     2341 ggtagacagt tttggcatct ggcggaagac ggtgcggaag tcggtcactt ttgagagatc
     2401 atcacttaag cggccataac ccacagcagt tccggctttg ccatcatcgc caacttcgga
     2461 ataacttaac cagatgcggc gagactgagc aaaatcaggc gctaaaacca cgtccagcag
     2521 gccgccctgc ccgtgcgccc aaacgtccgg aactccggaa agcggcgcag ataatccttt
     2581 tcctgcttgc cagtgacgca actcgccgcc gcgcagagtg attaacatac cgtgattatc
     2641 gggtaaaaag gccagtgccc agggatggtc gagtttgtct tgcagtactt cgacatttac
     2701 cgttgcagga gccgcccaga gagcggaaga aagacaaata aggggcacaa ggaaaaagga
     2761 ttgtcgatgc ataacgcctc cggattgact ctggcttaaa gcgtagtcag tggaggagat
     2821 aacaaattca tttttacaaa aacttaaaca tgaaggggga gacgctttct cccccttagt
     2881 tttcaggcct tctcaagcat ggcgtgcttc tgcaggctct ggatactcag cgttaagctc
     2941 atcagacaat tttcaagctt atcggcgttg acggtaataa cagtcgggca atcatggtgc
     3001 ccactcatca aacatactgc ggctgtcgct aatgcttctt cagcatgatg aagagcactc
     3061 cactcttcct gatccagatg aagattcaac cgcagcgatt tatcgtgcag ttcgcgattc
     3121 agtttaaaaa agttatctcg tagatgattg ctttcgctga cggacatgta tccttttgcc
     3181 ttcctcagtt gcacgtatgc agcgaggtca acgcgtgcgt cgatcaaccg gttcagcaga
     3241 tcgattcgct gtctgtcaac gaacatactt cgatcctcct ctttccagcc ttgttgcaat
     3301 taaatagtat ggctattttt tgagcaaaat gctgtctagg atcaattctt cacgccgaat
     3361 taacaaaatt tacatttcct tcgcctccgc cagcgaatga gattgcgata caaattttat
     3421 ccagaactct gtagaatccc tgccctgacc tttcctgtaa ctgaatattt tttaagctat
     3481 gagcaaagta actccccagc cgaaaatcgg ctttgtttcc cttggctgtc cgaaaaacct
     3541 tgtcgattca gagcgtattc tcaccgaact ccgcactgaa ggttatgacg tggtaccgag
     3601 ctatgacgat gcggacatgg tgatcgtcaa cacctgcggc tttattgaca gcgcggtaca
     3661 agaatcactg gaagccattg gtgaagcgtt gaatgaaaac ggcaaggtaa ttgtgaccgg
     3721 ttgtctgggg gcaaaagaag atcagatccg cgaagtccac ccgaaagtgc tggaaatcac
     3781 cgggcctcat agctatgagc aggttctgga gcacgttcat cactacgtgc caaaaccgaa
     3841 acacaaccca ttcctgagcc tggtgccaga acaaggtgtg aaactgacgc cgcgtcatta
     3901 tgcctatctg aaaatttcgg aaggctgtaa tcaccgctgc accttctgca ttattccgtc
     3961 tatgcgcggc gacctggtga gccgtccgat tggcgaagtg ttaagtgaag cgaaacgtct
     4021 ggtagatgcg ggcgttaaag agattctggt gatctcgcag gatacttccg cctatggcgt
     4081 tgatgttaaa catcgtactg gcttccacaa cggcgagccg gtaaaaacca gcatggtcag
     4141 cctgtgcgaa cagttatcga aactggggat ctggacacgt ctgcactacg tttaccctta
     4201 tccgcatgtg gacgacgtca tcccactgat ggcagaaggc aaaatcctgc cgtatctgga
     4261 cattccgttg cagcacgcca gcccgcgcat tctcaaactg atgaagcgtc cgggttctgt
     4321 agatcgccaa ctggcgcgca tcaaacagtg gcgcgaaatc tgcccggaac tgaccctacg
     4381 ctcaaccttt attgtcggct tccctggcga gacggaagaa gatttccaga tgctactcga
     4441 cttcctgaaa gaagcacgtc tggatcgcgt tggctgcttt aaatacagcc cggttgaagg
     4501 tgcagacgcc aatgccctgc ctgaccaggt tccggaagaa gtgaaagaag aacgctggaa
     4561 ccgtttcatg cagctgcagc agcagatttc cgccgagcgc ctgcaagaga aagtgggccg
     4621 tgaaattctg gtgattatcg acgaagtgga cgaagaaggc gcgattggtc gcagcatggc
     4681 agatgcaccg gaaatcgacg gcgcggttta tctcaatggt gaaaccaaca ttaagccggg
     4741 tgatatcctg cgtgtgaaag tcgagcacgc cgatgagtac gatttgtggg gtagccgggt
     4801 ttaattaatt caggccgggc aaaatcattt gtccggcctg cgaataatga ttactgccag
     4861 ctaatctgtg ccctgccgtt tttcttactc ttatacagcc gctcatccgc cagcattttc
     4921 aagcgttcga cggtagtaca tttatttgaa tgcgctacac cgatactggt gcttaaatta
     4981 atgcttccca cctggaaatg aacgatttct tttagtatcg atagcaactc ttccagcgtg
     5041 ccgccagtat aaatcaccgc aaattcgtca cctccgaagc ggtagacttt acctttatct
     5101 accgggatct gtgatttaag gattttcacc actttcatca acacttcatc gcccagcaaa
     5161 tgaccgaagg tgtcgttaat gtttttgaat ttatcgacgt caaacagaca caaataaccg
     5221 cctttttccg gcagcgcttt taaatcttca tcaaaggatt ttctgcccat tgctttggtc
     5281 aggtcgtcca ggaacgccga gttatacata ctgtagagtt ttttgacatc ggcagccgaa
     5341 aagaaaagcc cgccatccag tacgcgttca aaatggttat taatgatggc ttctttctcc
     5401 acaataccac gattaatcac tctgcgtacc agatagaggc aactggcacc cgatatcact
     5461 gccaccaatg cggcaaagaa aataatcaat gtacaaaccc gcaaataaac atctttttta
     5521 tacgcaaata cgtacaatcc ggtcggcaaa taggcgaagt gctccatcgc ttccgaaacg
     5581 gtgaagatgg cgtttttagc attatattca tctccggtca ccggtttacc gttcttaaat
     5641 agagagaaca tcaggttctc gctgtccatc gccagcgatt ttgacagtaa agaaaaacgc
     5701 tccagaccaa gcaaaaatat cagactacga ttgtgagtta ccggatagag gtaatagacg
     5761 aaggttttac cgtcaaaaga gataaa
//
LOCUS       DADMGC010000190         5717 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.190, whole genome shotgun sequence.
ACCESSION   DADMGC010000190 DADMGC010000000
VERSION     DADMGC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.190"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="JNZ73_003870"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="JNZ73_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HAY8733971.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="JNZ73_003871"
     CDS             578..1027
                     /locus_tag="JNZ73_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY8733972.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1260)
                     /locus_tag="JNZ73_003872"
                     /pseudo
     CDS             complement(1028..1260)
                     /locus_tag="JNZ73_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
     gene            complement(1346..1519)
                     /locus_tag="JNZ73_003873"
     CDS             complement(1346..1519)
                     /locus_tag="JNZ73_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HAY8733973.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1714..1809
                     /gene="yncL"
                     /locus_tag="JNZ73_003874"
     CDS             1714..1809
                     /gene="yncL"
                     /locus_tag="JNZ73_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HAY8733974.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2211..3020)
                     /locus_tag="JNZ73_003875"
     CDS             complement(2211..3020)
                     /locus_tag="JNZ73_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HAY8733975.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3230..4655)
                     /gene="patD"
                     /locus_tag="JNZ73_003876"
                     /pseudo
     CDS             complement(3230..4655)
                     /gene="patD"
                     /locus_tag="JNZ73_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
     gene            complement(4677..5383)
                     /locus_tag="JNZ73_003877"
                     /pseudo
     CDS             complement(4677..5383)
                     /locus_tag="JNZ73_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(5373..>5717)
                     /locus_tag="JNZ73_003878"
     CDS             complement(5373..>5717)
                     /locus_tag="JNZ73_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY8733976.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1498 a         1444 c         1269 g         1506 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt gtatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcacgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagccg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcgttggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg tgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcgac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggccgctt ccatatcttc
     1081 tttatgcttg cgaccgtgat gagcaatagc ggtgcgcaaa cgctgctgct gggtgtagcg
     1141 atcttcccga cttaactccg cgtcatcgct cagggcgatt aataattcgt tcatatgggc
     1201 aatgacgctc tcttcaatcg cggcatccac gcgcgcgatg acttcatcca gatgcgacat
     1261 actttctcct tagccacttg tgaaatttat cgattaatcc aaatgatata agtctgcgtg
     1321 gtgccgtcaa gccagtgagt aagatttaaa acagtgaaaa caacagcgcc accgggaaac
     1381 tcatcggcca tgtggccccc accagaaagg cgcttaagaa acggatgcgt ttcttatcgt
     1441 gagaaagaaa ccaggtgata agaaatgcga ttgctgccat aaccgcataa aaaacaagca
     1501 tatgttgata gagagacatg tacgggcgtc cagccatatg aataaaacgc gcgcaaatat
     1561 gcgccatttt ttgacgtgca tcaagttttt tcaccagttt gagaaaaaat catcaaacag
     1621 caacttgccc ttatccatct tcagaatgag tattattgcg gattttctta acccgtacta
     1681 tacacatagg ggcaatgata aaaggtggca aaaatgaatg tttccagtag aactgtagta
     1741 ctgataaatg tctttgctgc tgttggtttg tttactctta tctctatgag atttggctgg
     1801 tttatttgat gtagtgattt tgcacgcagc acgtactgtc agagaaatca actctctgac
     1861 agtacgcaag atcttctcat actctccaag tttgcaacat tatccttaat atttacttct
     1921 ttttgctggc atctacattg aaatgagtag aaaacatccc cctactgtcg tctatacaaa
     1981 cagcttactg attcaaacgg aatcttgttt tcacattttt ctatcacgaa tactttatac
     2041 gcgcgggagt ctataaatgc tttatacttt caaggaataa cgtaaatata aaaagatggc
     2101 gtaaagtata tgcatggcag gatgtttgca acctccttga tgacgctgcc ccattgaaat
     2161 tgcaaacccc gccctggtta tcttgcccat ttatcatgca aaacagataa ttataggcct
     2221 ctgaatacct cagaaagatt gaatttttta tagtccctct cttgaatatt tccatttgat
     2281 atcaatcgat tcatctcttc aacaaaataa tttatatcat cacacttgtt attatccgga
     2341 agatctgagt agttataatt ccaccatttt attctcaaca gttcatcaat aacatttgaa
     2401 tcaaacctaa atctgattat ttttgcagga actcctgcaa ctatggcata tgggggcaca
     2461 tctttggtaa caacggaatt ggcagcaata acagcaccat cacctattgc gatgccacct
     2521 ttcaatacaa catcatttcc tatccagaca tcatttccta taatcggatt ttcaggtttg
     2581 gtaatgaatg gtttcaatgt ccagctacgt tcaaattcac tcatagcaaa cttatcaaat
     2641 tcaccgttat aagtcatcat gtgagtagta aaacgattca aaggatgatt tccacccatt
     2701 atttttacat ttgatgcaat actacaaaat cgacctatac gtatattccc tgaaaaatgg
     2761 caggtcggaa atgaaaaagc gccgacactg gagaaatttt tcacaggcat tgttgaatat
     2821 tcttcagcaa taacattatc ttcaattgtt atcttgtcac cttttgagaa aagaaataaa
     2881 ttcctttctt tatacgactt cttcccctta tgatttaaaa agattttatt atccatacaa
     2941 aactcaaagt gttttttcgt ccatctgaag gtaagggttt tatttttatc ttccgtgaat
     3001 ttgtacaatt gaactttcat tgttaatact tctacttata aattattagt ttaataacga
     3061 tactctaaag ccttatgcta atcaactgtg acaggatgaa aaatcgccat caaacattta
     3121 gcctaatttt tacgccagtt attaatgtaa tcattctaat acttaaccgc cattaaactc
     3181 agaacctaaa acgatagtgg ccgtcctata acagccacta acatctaact taatgtttaa
     3241 ccatgacgtg gcggacgacg gtgtaatcct ccagcccata aagtgacata tccttgccgt
     3301 aaccagaaag tttctgccca ccgtgcggca tttcacttac cagcatgaaa tgggtattga
     3361 cccaggtaca accatattgc agccgtgcgc tgacgcgatg cgccctgccc acatctttcg
     3421 tccataccga agatgcaagt ccgtactggc tgtcattcgc ccagttcacc acctgttctt
     3481 cgttgtcgaa gggcgtaaca ctcactactg gaccaaatac ctctttttgc aggatggcat
     3541 cgtcctgtaa tgcgccagcc agcagcgtcg gcgcatagta atagccatta cccttgcgct
     3601 tttcaccgcc agtgatcact ttgatgtgcc ctgtcgcttt cgcctcttct actgccttgc
     3661 tgacgcgttc gagatgcgcc agcgagctta aaggtccaag ctccgtagac tcgtcatccg
     3721 gcgcaccaga ttttaacgtt gccaccgcag gcacccagtt tttccaccag cgtatcgtaa
     3781 atgccttttt gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc attgtaatag
     3841 ccaaatgtac gtacaccttc gaccactgct tcaatatccg catcatcaaa aacaatcact
     3901 ggcgctttgc caccaagttc catatgagta cgcttaatgg acgacgcggt atggctgatg
     3961 atgtgctcgc cggtggcgat agagcccgtc agcgacacca tccgcacttt gggatgaccg
     4021 gtcagcggat cacccaccgt tttgcctctg ccaaacagta tgttaatcac gcctgccggg
     4081 aagatatctt tcgccagctc tgccaacttc aacgcggtca gcggggtaat ttctgatggt
     4141 ttaagcacta cgcagttccc tgccgccagc gccggagcaa gtttccacgc ggccatcatc
     4201 agcggataat tccacggtgc gatagaagcc acgcccccca acggatcgcg acggatcatc
     4261 gaagtatgac cttcaaaata ttcacctgcc gccagaccat tcagacagcg cgccgcaccc
     4321 gcgaaaaagc gaaaaacatc gacaatcgcc gggatttcat cattgaacgc actatgcagc
     4381 ggtttgccac aattacggga ttccagttcg gcaaaaacct gaccattttc ttcgataaca
     4441 tccgccagtt tcagcaggca ttccgcacgc gctttcggtg tggtttgccc ccattcagca
     4501 aatgctgcat ctgccgcaca cacagcagca tcgacctgct ctgcggatgc ctcggcaatc
     4561 tccagtaaaa cgtcccccgt tgccggatta tagacaggct gtttttcccc ttcgccgcta
     4621 accagttctc cgttaatcag taacttatgt tgcatagatt attcctgtat cagtggttat
     4681 tgaccattgt cgccttcgcg ggttagccac caggccccca ggatcggcaa ggttgttacc
     4741 aacataacca gcagtgccac cacgttggtt accggtacat cacgcggtcg cccaagctga
     4801 ttgagcaacc acaacggtaa cgttcgttca tgacctgccg taaaggtcgt aacgatgatt
     4861 tcatcgaacg acaaggcaaa cgccagcatt cctcctgcca gtaacgccga actgagattc
     4921 ggcaacacta cgtagcggaa ggtttgccag ccattggccc caagatccat tgacgcctca
     4981 accagactcc acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac caccactacg
     5041 caaaaagtcg catgaccgac cacgatggtg aaaaatcccg gctccagatt gatggtttta
     5101 aaggcggtta atagcgccag accagtgaca atgcccggca gcgcaatggg cagcagcaat
     5161 aacagcgaaa tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc agctgccagc
     5221 gtccctaaca ccagcgcagc gtcaggccct gcggtggaaa actaaacgcc gcatcttcag
     5281 tgttaaacgc ataggcggcg attatcagga tgggaaaatg taggaaaaca acgccgcccc
     5341 aggccgccag tttgaggaaa aacggtgcgc gttcagagtg catcgaacgc tcccagacgt
     5401 ttcacgaacg ccaggtacag tgcgatgaga ataatcggca ccagggtgaa tgccgccgcc
     5461 atcggcatat tgccaatcgc cccctgctgg gaataaacca tattgccgat aaaatatcct
     5521 ggaggaccaa ccagctgcgg gacgataaaa tcgcccagtg tgagtgagaa ggtaaagata
     5581 gagccagcgg caatacccgg gattgccagc ggcagcacca catagcgaaa ggtttgtcgt
     5641 ggacgtgcgc cgagatcagc cgacgcctgc aacaatgacg gcggcaaacg ctcaagcgcc
     5701 gcctgaacgg gcaggat
//
LOCUS       DADMGC010000191         5708 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.191, whole genome shotgun sequence.
ACCESSION   DADMGC010000191 DADMGC010000000
VERSION     DADMGC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.191"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            57..1525
                     /locus_tag="JNZ73_003879"
                     /pseudo
     CDS             57..1525
                     /locus_tag="JNZ73_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            complement(1561..1764)
                     /gene="ydfZ"
                     /locus_tag="JNZ73_003880"
     CDS             complement(1561..1764)
                     /gene="ydfZ"
                     /locus_tag="JNZ73_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HAY8733977.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1942..2628)
                     /locus_tag="JNZ73_003881"
     CDS             complement(1942..2628)
                     /locus_tag="JNZ73_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY8733978.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSNWFSEE"
     gene            complement(2717..3463)
                     /gene="ydfG"
                     /locus_tag="JNZ73_003882"
     CDS             complement(2717..3463)
                     /gene="ydfG"
                     /locus_tag="JNZ73_003882"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HAY8733979.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3600..5645
                     /gene="dcp"
                     /locus_tag="JNZ73_003883"
     CDS             3600..5645
                     /gene="dcp"
                     /locus_tag="JNZ73_003883"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HAY8733980.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARHRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
BASE COUNT         1427 a         1357 c         1474 g         1450 t
ORIGIN      
        1 tttttattca tataacaaaa catatagctt gccatattta aatttaagga attgttatgg
       61 gaaataattt gttatcagca aaagcgacac tccctgttta tgatcgtaat aacctggccc
      121 caagaattgt tcatttaggc tttggtgcat ttcaccgtgc gcatcagggc gtgtatgccg
      181 atattcttgc tacagaacat ttcagtgact ggggatatta tgaggtcaac ttaatcggcg
      241 gcgaacagca aattgccgat ttacaacagc aagataatct ttataccgtt gcggaaatgt
      301 cggccgatgc gtggacggct cgcgtcgttg gcgtcgttaa aaaagccttg cacgtacaga
      361 tggatggctt agaaaccgtg ttggctgcga tgtgtgaacc gcaaatcgcg attgtctctc
      421 tgacaatcac cgaaaaaggg tatttccact ctccggcgac cggacagtta atgctcgatc
      481 acccgatggt cgctgccgac gtgcaaaatc cccaccagcc gaaaacagca acatgggtga
      541 ttgttgaggc gctggctcgc cgtaaagcgg caggacttcc cgcattcccg catttaccgt
      601 catgtcatgt gacaacatgc cagaaaacgg tcatgttatg cgtgacgttg tcacttccta
      661 cgcgcaagcc gttgatgtaa aactggcaca atggatcgaa gataacgtga ctttcccatc
      721 aacaatggtg gaccgtattg tgcccgcagt ggcagaggat acgctggcga aaatcgaaca
      781 acttaccggt gtgcgcgatc ctgctggcgt tgcctgtgaa cctttccgcc agtgggttat
      841 cgaagataac tttgtagccg gacgtccgga atgggaaaaa gcgggagccg aactggttag
      901 tgatgtgctg ccttatgaag agatgaagtt gcgcatgctc aacggcagtc attcattcct
      961 ggcatatctg ggttatcttg ctggatatca gcacattaat gactgtatgg aagatgaaca
     1021 ttatcgtcat gcggcgtatg gcatgatgtt gcaggaacaa gcgccgacgc tgaaagtgca
     1081 gggcgttgat ttgcaagatt acgctaaccg attaattgca cgctatagca acccggcgtt
     1141 acgtcatcga acctggcaga ttgcgatgga tggcagccag aaattgccac agcggatgtt
     1201 ggattctgtt cgctggcatc tggcgcatga cagcaagttc gatctgctgg cgctgggcgt
     1261 cgcgggttgg atgcgttatg tcggtggtgt tgatgaacag ggaaatccga tagaaatcag
     1321 tgacccactg ttacctgtta ttcagaaggc tgtacaaagt agtgccgaag ggaaagcgcg
     1381 cgtccagtca ttgctggcga ttaaggcaat ctttggtgat gatttgccag acaatagctt
     1441 gtttactgca aaagtgacgg aagcgtactt gtctttatta gcgcatggcg cgaaagcgac
     1501 cgtggcgaaa tattccgtga agtaaaaaaa gcaatgccgc catcggcggc attcacacac
     1561 ttagttcatg ccgagacgaa tcaggtccag aggtgccagt ttctcttcac aaccttcaac
     1621 aactacggtt tttccgcggc ggatttgctc agcggtcaga ccttcagtat caatcgacag
     1681 gatcttgcca gtgtgaccgg tgccgctaac cataacacgg ctgccagtgg tgattgcgtt
     1741 acggttacga tcgtaggtcg tcataggtgt tttctccttt ctgatttaca gtcgaagccc
     1801 acgtaacggg cgcaatataa atactcctgt cgagtgaaat attttttgtt tttgatcaag
     1861 ctcacatctt ttttgatgca tgcatgagcc gtaaggcggg gggaaaagcc tctcatcccg
     1921 gatgggatga gagtaaatga attactcttc gctaaaccag ttgctgtttt cctggcggat
     1981 ctggcgcacg gattcgctga tttcctgcaa atgttgcgtc attgcacgtt ctaccgcatc
     2041 gccatcacgt ttttgcaggg cagagaaaat atcaagatgc tggcgtaaca gcatttctgg
     2101 tggagaaaca tggtcgaaac tcatatagcg cacgcgatca acggtcgctt tcaggttctc
     2161 aatggtatcc cacgccagtt gacagtcggc aatctgcgtc aggagttgat ggaagttgtc
     2221 atcaagttca aaaaaatcat ccagttgctt gcgctcaatg gcaatgcgtt gctggtgaag
     2281 attttgttcc agttggtagc actggctttc ggtaatcatg ctcgccgccc gacgcgccac
     2341 cgcgcactca atggcctgac ggataaaact gccgttgcgc acctgggcca tggaaatttt
     2401 gttgacgtag ctgccacgct gcggacgaat ttgaatcagg ccgttttccg ccagtttaat
     2461 aaaggcttca cgaaccggct ggcgtgacac attgaaacga acagaaactt ctttttccga
     2521 caacggtgtg cctggtgcaa tcaggcaatg gacaatgtcg cgacgaagaa tacgataaat
     2581 ctgctgattg acaggctgtg tgggattaag ttgcgtttcg acggtcattc gttcttgctt
     2641 gtgagtgagt taactgcatg agtctaccac ttttttgtga caagcaataa cccggcagtt
     2701 acgccgggta taaaaattac tgacggtgga cattcagtcc ggcatagctt tgggtaaccg
     2761 gcatcatttc cagggtattg atattgacgt gagcaggcag cgttgacacc caccagacgg
     2821 cttcgctgac atcttctggc gtcaatgcaa cggtattttg ataggttttt tccgctttac
     2881 cgtcatcgcc tttaaagcgg acattggaaa actcggtgcc acccaccaga cccggttcga
     2941 tgtcggtgac gcgcaccgcc gtaccatgca gatccgtacg cagattcagg ctaaactgac
     3001 gaacaaacgc tttcgtcgca ccgtaaacgt taccaccggc atacggccag ctgcctgccg
     3061 ttgagccaat gttaataata tgaccatgat tacgttcaac catacccggt aagacggcgc
     3121 gcgtcatata tactaggcct ttgttgttgg tatcaatcat cgtttcccag tcttcaacgc
     3181 tggctttatg cgcaggctcc atgcccaacg ccaggccggc attatttacc aggatatcaa
     3241 tattgctcca ctcggcaggg agcgatgcca gcatctcttc aatagcggcg cggttgcgaa
     3301 cgtccagttg ggcgatatac agattatctc ccagttcgtc ttttaactcc tgcaaccgct
     3361 cctggcgacg gccagtggcg ataactttat gcccttgttg aataaaacga cgagtaatgc
     3421 attcaccaaa acctgccgtt gctccagtta ctaaaacgat catctcactg ttcctcaacg
     3481 cttttgtgtg tcaataacat agcacgtgat gcttaagagc gggtaatact ggtttgcaag
     3541 attgcactcc gttatcagta agcctacgct atggaacacc tcgcgttcag gagaagaaaa
     3601 tgacaacaat gaatcctttc cttgtgcaaa gcacactgcc gtatctggct cctcattttg
     3661 atcaaattgc caatcatcac tatcgcccgg cattcgatga gggaatgcaa caaaagcggg
     3721 cagaaattgc tgccatcgcg cttaacccgc aaacgcctga tttcaacaat actattctgg
     3781 cactggaaca aagcggagaa ttaattaccc gcgttaccag cgtctttttt gcgatgactg
     3841 cggcgcatac caatgatgaa ttacagcgtc ttgatgaaca gttttccgct gaactggcgg
     3901 aactggctaa tgatatctat ctgaacggtg aattattcgc gcgggtagat gctgtctggc
     3961 agcgccgtga atccctgggg cttgatagtg aatccatccg cctggtggag gttattcatc
     4021 aacgttttgt ccttgccgga gccaaacttg cacaagctga taaagcaaaa ttaaaagtac
     4081 tgaatacaga agctgcgacc ctgaccagcc agtttaacca gcggttactt gcagcaaata
     4141 aatccggcgg tctggttgtg aacgatatcg cgcagctggc aggaatgagt gagcaagaga
     4201 ttgcgctggc ggcagaggcg gctcgcgaga aaggtctgga taacaaatgg ctgattccgc
     4261 tgctgaatac cacccaacaa ccggcgcttg ccgaaatgcg cgatcgtgcg acgcgtgaaa
     4321 aactgtttat tgcgggctgg acgcgagcgg aaaaaaatga tgccaatgat acccgcgcta
     4381 tcattcaacg tctggtagag attcgcgcac agcaggcgaa actgcttggt tttcctcatt
     4441 atgccgcatg gaaaatcgcc gatcagatgg caaaaacgcc agaagcagca ctcaacttta
     4501 tgcgggaaat tgttccagcg gcgcgtcaac gtgcgagcga tgaattagcc tccatacagg
     4561 cggttatcga taagcagcag ggcgggttta gcgcgcagcc gtgggactgg gcattttatg
     4621 cggaacaggt acggcgggag aaatttgatc ttgatgaggc gcagctcaag ccatattttg
     4681 aattaaacac ggtgttaaat gaaggtgtat tctggaccgc gaatcagctc ttcggtatta
     4741 agtttgtcga acgttttgat attccggtct accatcctga cgttcgtgtg tgggaaattt
     4801 ttgatcataa tggcgtggga ctggcgttat tttacggtga tttcttcgcc cgtgattcaa
     4861 aaagcggcgg tgcatggatg ggcaattttg ttgagcaatc aacgcttaat gaaacgcatc
     4921 cggtaattta taacgtctgc aattatcaga aacccgctgc cggtgagcct gcgttgttac
     4981 tctgggatga tgtaataacc ttattccatg aatttggtca tacgctgcac ggcctttttg
     5041 cccgccatcg ttatgccacg ctttccggca ccaacacgcc gcgtgatttt gtcgaatttc
     5101 cgtcgcaaat caacgaacac tgggcaacgc atccgcaggt attcgctcgc tacgcccggc
     5161 attatcagag cggggcagca atgcctgacg aactgcaaca gaaaatgcgt aatgccagcc
     5221 tgttcaacaa agggtatgag atgagcgaac tgcttagcgc cgcacttctc gatatgcgct
     5281 ggcattgcct ggaagaaaac gaagcaatgc aggatgtcga tgattttgaa ttgcgggcgc
     5341 tggtggcgga aaatatggat cttcctgcta taccgccacg ctatcgcagc agttatttcg
     5401 cccatatttt tggtggcgga tatgctgcag gttattacgc ttatctgtgg acgcaaatgt
     5461 tggccgatga tggttaccag tggtttgttg agcagggcgg attaacgcgt gaaaatgggc
     5521 agcgttttcg cgaggcgatc ctttccagag gtaacagcga agatctggaa cgcctgcatc
     5581 gacaatggcg cggtaaggct cctcagatta tgccgatgct gcaacatcgt ggcttgaaca
     5641 tataaacacc accaatggct ggcctggaga tatcgccagg ccagcctggt tatagcagta
     5701 attgcagc
//
LOCUS       DADMGC010000192         5687 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.192, whole genome shotgun sequence.
ACCESSION   DADMGC010000192 DADMGC010000000
VERSION     DADMGC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.192"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(149..568)
                     /locus_tag="JNZ73_003884"
     CDS             complement(149..568)
                     /locus_tag="JNZ73_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733981.1"
                     /translation="MHQLRHPLLGCVFCNECDPHPFAPDPLQIFHHYYEWVRPCFPIR
                     AGNRFSRSLRWSGPGSCCLHTGCRAVSKQVSSALIRGRLYGPILASSKISMLHRTVYF
                     RSASRTPRDSRSLPFPESLTTSFLRMKQHRVVWSVLL"
     gene            618..1796
                     /gene="ltrA"
                     /locus_tag="JNZ73_003885"
     CDS             618..1796
                     /gene="ltrA"
                     /locus_tag="JNZ73_003885"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY8733982.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDVTRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1879..2058
                     /locus_tag="JNZ73_003886"
                     /pseudo
     CDS             1879..2058
                     /locus_tag="JNZ73_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2088..2570)
                     /locus_tag="JNZ73_003887"
                     /pseudo
     CDS             complement(2088..2570)
                     /locus_tag="JNZ73_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2826..3806)
                     /locus_tag="JNZ73_003888"
     CDS             complement(2826..3806)
                     /locus_tag="JNZ73_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAY8733983.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3806..5005)
                     /locus_tag="JNZ73_003889"
     CDS             complement(3806..5005)
                     /locus_tag="JNZ73_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAY8733984.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5327..>5687)
                     /locus_tag="JNZ73_003890"
     CDS             complement(5327..>5687)
                     /locus_tag="JNZ73_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY8733985.1"
                     /translation="TFHPSLTQHWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTP
                     FCIGMVTSDDWGSYAREVPKEKHLTGKIFTQRIERNNRTLCTRIKRLARKTICFSRSV
                     EIHEKVIGSFIEKHMFY"
BASE COUNT         1563 a         1295 c         1357 g         1472 t
ORIGIN      
        1 ccgttccggt ccaggagccg ggcagcggta aaacaaggac tgcccggctg tgggtctacg
       61 ttcgtgatga ccgtaacgcc ggttcggaaa ttgcagcata agttatccat gctctgaggt
      121 gggtggaggt gggccctccg gtcagggttt acaggagcac tgaccaaacg acccggtgct
      181 gcttcattct aaggaatgag gtggtgagcg actcgggaaa aggcagagat ctactgtcac
      241 gtggggttcg ggaagctgaa cgaaagtaaa ctgtccgatg aagcatcgaa atcttagatg
      301 atgccaaaat ggggccgtac agccgtcccc tgataagcgc agaagacacc tgcttactga
      361 ctgcgcggca tccggtgtga aggcagcagg atcccgggcc agaccatctc agggaacgtg
      421 agaacctgtt tccggcacgt atcgggaaac aggggcggac ccattcgtag tagtgatgaa
      481 agatctgtaa tgggtctgga gcgaagggat ggggatcaca ttcattgcag aagacacaac
      541 ccaaaagggg atgacgtaac tgatgcaacg aaaatcattt gaaataccaa aagcactggt
      601 ctgggcatca tatctggatg tgcgtcgcaa caagggagcc ccccggatgc gacgggcaga
      661 ctttgaaaat gttcgaccag caacgtgatg gcaatctata caagatctgg aaccggctat
      721 gttcgggaac atggtttccg ccaccagtgc tggaaaaacg gatcccgaag tcgaatggca
      781 aggaacgcat tctggggatc ccaacagtat ccgatcgaat tgctcaggga gcgataaaac
      841 ttttcatgga agaaaagctt gacccgattt tccatgctga ttcatatggt taccgaccag
      901 gcaaatcagc tcatgacgct ctgaaacaat gtgccatccg gtgctggcgt tatagctgga
      961 tactggaagt ggacatcagt gcgtttttcg accatgtgag acatgatctg gtgctcaaag
     1021 ctctggaaca ccacgggatg cctaaatggg tcatcctgta ctgtcgacgc tggatggaag
     1081 cgccaatgca gagttgtgaa aatggagaat taataacccg aacacgaggc actccgcagg
     1141 gcggggtcat tagtccgcta cttgctaact tgttccttca ctatgcattt gatttgtgga
     1201 tggaaagaga atatcggggg gtaccgtttg agaggtacgc tgacgatatt gtagtacatt
     1261 gttcacgaat gagtgatgta acaaggctaa agaacaggtt atcggagcgc ttctcggaag
     1321 tcgggctggt cctgaatgca gggaaaacga atatcgccta cattgacacg tttaaaagga
     1381 gaaacgtcgc aacgagtttc acctttctcg gatatgactt caaagtgcgt acgctgaaga
     1441 atttcaaagg cgaacggtac cgaaaatgca tgccgggtgc gtcaaatgca gcaatgcgca
     1501 aaataacaga aacaatcaag aagtggcgta tacatcgctc aacagctgag agtttgctgg
     1561 attttgcgag gcgctacaat gcgatagtga gaggctggat cgagtactac ggaaagttct
     1621 ggtccagaaa tttcaactat cgactgtgga gtgcaatgca gtcacgtctg ctcaagtgga
     1681 tgcagtctaa atacagactt tcgaaccgga aggctcagcg aaagctgacg ctggtaagga
     1741 aggagtatcc gaagctattt gttcactggt atttactacg tgcatcaaat gagtgatcaa
     1801 gagccgtatg acgcgagagt gtcacgtacg gttccgtgag cagccgggga gtgaaattct
     1861 ctccggctga ctcgactcgc gggttccgac agcgatgacg aacatgcggc ggtgttgtac
     1921 tcgctgatcg gcacatgccg tctgaacaat gtggaaccag aaaaatggct gcgttacgtc
     1981 attgagcata tccaggactg gccggcaaac cgggtacgtg atctgttgcc ctggaaagtt
     2041 gatctgagct ctcagtaaat atcaatacgg ttctgacgag ccgcttactt ttggcgtaat
     2101 tctgctgcgc ctttactgaa cacaatgccg tattcagcaa gaagtccacg aatgtgattt
     2161 atcagttgtg tcctttccat tgaaaccaac cgacgcatat tcaaaaaagt ctggacgtcc
     2221 tgttgctcca gagtttttat tggtacgggt cgtatagttc catgttggca ggcctcagct
     2281 attgcctgtg cgtcgttata atcatttttc tggcaccgct gataagcttt aacatgttgt
     2341 gccggaagca atattacatc atggtcgagt ttcctgattt cccgggccca gtaatgagat
     2401 gccccgccag cctccatcgc aacagtcatt ttaggctgtt gcacctgcca gttaagcagt
     2461 tgattgcgag aaagtttggt ctttcctgcg ggtttgccat gagcattgat actgaaaaga
     2521 taaaagttcg ttttagccag atcaatgccg gtgattttct gttcagacat ggtatcccgc
     2581 ttacttttgt ggttaaagtt tatccatcat ggcgcaattg aaggctgccg tgaaagaggg
     2641 ggaagactat ctcatcggtt actgttaaac ggctaagaga aactatcttt ttcaagcaaa
     2701 gaagatacgg tgaaacaggc gatttcaccg tgaaatactg ttcaaaaaaa acgctaataa
     2761 aagcaaaatt tcttttaaaa cagccaatta aaaactggtg agttgctggc gaaaaacttg
     2821 aattttcaca gcgaattcag attatctttt agtacaagag caatcatacg atctaactta
     2881 tcctgaactt ccaacggtaa tctgccgaat tcatatgaga acgtacgtcc ctttactcgt
     2941 tttcgagcaa atttatcttt actatcaaat ttccataaca aagttacaac cgctttatcc
     3001 tttactcctg aatcaagcaa tgaagacatt tccttggtta taagccgcat aatactgttt
     3061 ttaacctcgt cttcagaaag attgtcatca gcagtaataa gctcaatact cggcgagata
     3121 ttcttcacaa cttcatcaat agatatatca cccagcctca gtcgttcact taattcagcc
     3181 agttgcctgt aatcggcgta tgtcagctca gactgaacag gaaaaagaga cacaaaatct
     3241 ttaggtacgg acgctgcttg gagggcacga gtcaccttag ctgccgacaa tccctctctt
     3301 tccgcaattt gcttctggtt cataccggcc tctttgagac gtactaaacg caacccaatt
     3361 tcacgaatat tatgttcaag tgacgtctgc aaatctttcg ccagatgctg agcttcagaa
     3421 acagtaagct catcatccgt tacaagtaca cgtaatccaa ccttgcatag caaagctgct
     3481 gcccgacgac gggaaccatc caaaatctca ataagatcgc cagtccgaat cccaatacat
     3541 ggatagaatt gttgtaaacg tatagttctg gtgatgtctt ttagtgagtc cggcgtcagg
     3601 gctgtctgtt cgcggccatt cacctcttca accacaaaag tttgagtgtc cactttgtca
     3661 tgatcaacct tgatctcagt aaattttgcc tttcggccag tattgagggt aaatacatga
     3721 actggagccg atatttcttc agtattattc aggatatttg tattcagcgt acggccaata
     3781 gtcggtcggt gctttctgtt ttccatcagt agttggccct tataaattca atacgatcaa
     3841 aaactgcttt agcgaaatct tctgctgcca tacgtgcatt tttcaaagct tcaccactgc
     3901 caacataagt aacaggattc gcagatatca cggtatcaaa cgactcgccg cttctctcaa
     3961 aaccatcaag acgtggcatt gaaacatcaa gcatgtcacc accgaaaatc tcttttgtca
     4021 ggctatggca gtatttatga tctgacttat tcgccagttt cgacatgaag ccaatatttg
     4081 cctgcaaacg gcatgaacat ccatcctgtt caataatctg tacaagttca ggtaaacgag
     4141 ccaaatattt aagcgtggaa tggaaatcaa cttgtgctgg cggcaccggt gtaaacatga
     4201 tatctgcggc agcaatggca tttttcaaaa atgcgtccag atgcgggcct gtatcaataa
     4261 ggatgaagtc gaaatcatgc ttcagcttat caataatatt ctcacgcaat atcgcgtgtt
     4321 tgttttgtcc taatagatgc tcctcgcaca gagtatccca gtttgatgca ataaaggcgt
     4381 cgtcaataga agccggcata acataaacgc cagggataac tgacggaacg ataaaatctt
     4441 ccagtaattc ctcacgactc acgttctgca acatagcctg cggtgcggtt gtatcaacca
     4501 gccccaccgc atgcaagtag ttcagaaaca tagtggctga tgactgggga tctaaatcaa
     4561 ggagcagaat acgtaaatct tctgacagca ggtgaggatg agcacgcagg gcgtgagcta
     4621 cggaaacact actcacggtt ttagacacac cgccttttag actaccgatg aatatggaat
     4681 atgcctcact ataacgatcg cgatatttag gaatgccacg atgtgcatag atatcgataa
     4741 tgttctggat ggttaatgca tattttgtcg cggttcctgc tgggcgtttt tcaaactgat
     4801 agccctgagc ctccatctca ccgactgcac catccacgct tgcgcgcgtt aattttggaa
     4861 gcttagctac agcagacttt gaatagactt gataaaaaac atcagcctga aactcctgct
     4921 tttgctcctg aatctgtttt gttagggcga gcaacatttt atctgcacgc tgcgcaacct
     4981 tacttaactg ctcaaaacta gtcattcccc ctccgtgcta tgtaggattt taacttatta
     5041 caaaaaaaac tgcattagca gggtaatgca tgcaagtaca tattgtacat gcttatatta
     5101 aaaatgcgcg cattgctgat ttattaatca tcggaatgaa tattactgta agatagtatg
     5161 cctttccggg agaaagacgc tgatttcccc cgaataatcc agttcaccac cacactccgg
     5221 acaacagctt tcttcaggct ccagatggtg tatttcgcgg gaaacatgtt ccggtaacgg
     5281 acggcgatga cgtgactgtc gcaacaggcg ggggtagtgc ttccaatcag tagaacatgt
     5341 gcttttcaat gaaggagccg atgacttttt cgtggatttc cacggaacgc gagaagcaga
     5401 ttgttttacg tgccagccgc ttgatgcggg tgcagagggt ccggttgtta cgttcaatac
     5461 gttgcgtgaa gattttgccg gtcaggtgtt tctccttcgg cacttctctg gcgtagctgc
     5521 cccagtcatc gctggtcacc atgccgatac agaacggagt caacagggct aacagttccc
     5581 gacaggtctc atcattccgt ggcccgaaag tgtaagccaa cacaccgcca gttttagtgt
     5641 tgtacgcata ccagagccag tgttgtgtca acgacggatg aaaagtg
//
LOCUS       DADMGC010000193         5599 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.193, whole genome shotgun sequence.
ACCESSION   DADMGC010000193 DADMGC010000000
VERSION     DADMGC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.193"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..410
                     /locus_tag="JNZ73_003891"
     CDS             <1..410
                     /locus_tag="JNZ73_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY8733986.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            442..1056
                     /locus_tag="JNZ73_003892"
                     /pseudo
     CDS             442..1056
                     /locus_tag="JNZ73_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="JNZ73_003893"
     CDS             complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="JNZ73_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HAY8733987.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTSGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWFSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKTFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYTGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1380 a         1402 c         1329 g         1488 t
ORIGIN      
        1 ccgtcaggca gcagcgaagc aaagcgttac tgacgtcgct gaatgaatgg atggtggaga
       61 agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc
      121 agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggac aataacacag
      181 cagaaagagc gcttcgtacc gtctgtctcg ggaaaaagaa ttacatgttc ttcggcagcg
      241 atcatggagg cgatcgcgga gctctgctgt acggactaat cgggagctgt cggttgaacg
      301 gtatcgatcc agaagcgtat ctgcgccata tcctgagcgt actgccggaa tggccttcca
      361 accgagttga cgaactgctg ccatggaacg tagttctcac cgataaataa gcgtcaatac
      421 ggccttccgt tgacgcttac agaactcctg tggcgcgaga gtgaaaaact gcgtcgttat
      481 aaaaaggagg cccggttgcc agttgccaaa acgctaagcg aatacgactt cattcagttg
      541 ccggaactga atggagctca gttccagcaa ctctgtgaaa cgacagactg ggttgatgct
      601 ggagaaaacg ttctgctgtt cggggacagc gggttgggga aaagccatct ggcggcagcg
      661 atcgtggatg gcgtagtagg ccagggctac cgggcccggt tcaacagcgc aggagagctg
      721 ttgcaggaac tacataaagc cagagctcag ttgaaactga atgagctgct actgaaactg
      781 gatcgctacc gggtgatagt ggtggatgat cttggctatg tcaaacgcga cagcgccgaa
      841 acgggtgtgc tgttcgagtt aatagcgcat cgctatgaac gtgggagcct ggtgataacc
      901 agtaaccatc cgttcagcat gtggggcagc atcttcgtgg atgagactat ggcggtggcg
      961 gcagcagacc gactgatcca tcacggatat atgttcgaac tgaaagggga aagctacagg
     1021 aaaaagacag cgaaggcagt aacaagcgtg acttgatgtc gcactgaagg ggcggccagt
     1081 atagttggcg ccagtcggca aaactagttg acgtctaata cctggacggt cacttctgag
     1141 aaaaggcatt tacacagaat cttaaacagg ctcacggagt tctttcagaa aaaggcctgc
     1201 cgaatggcag gcctatccca ttcgtcagaa aacataacgg aagttggcgt ttatcgcatt
     1261 atccacatta tatttcccga acgctgattt ctccagctcc agtcccaaac gcatattatc
     1321 cttaatttcc gcattcatcc ctaccgtcat cagcatcctg ctatcttttt caccttcgaa
     1381 acgtttctct ccggaagcat cctgcagaac cgtttctccg ttcgccagca ggtcgaactg
     1441 ataacccagc ccggctcgag ctgtgatttt ccagtcgtct ccggagaagg ctcttcccac
     1501 gtcaacacca gtacggccaa tcagtgggtt ataatccttg tctttcatgc tcagagccat
     1561 tccccggtct tcccagctaa aagcttttcc tgatacagaa ccgtaaacca gctctatctg
     1621 tggctccacc caggactctt tcgtcaggtg gtaacgataa ccaacttcag caccggtata
     1681 ccaggaatga gagctgtagt cttttgttcc cagtgaggca aaattcgccg tgtgctgatt
     1741 atcatgatgg agatatttac caatcaggtc aaaataagct ccggaattaa agagtgcaga
     1801 ggcatacaga ccgccaccca cggatttgtt ttttccactg aatgcgtggc tgcttgcatt
     1861 gctgtccgta taggtcagca atgcccccgt aaataaatcc acaccgtcca gctcatgctt
     1921 tctgtcgaca ccaatctgaa cgtgagtgta gttatcgctg tagtcaccat ctgccgaacc
     1981 ggtaccattc attatgcgtg cccagacacc ggcatccccc tgagtatccc gcaggtcacc
     2041 catacgtttg ttcaggttat tgacctctgt aaggaaggtc ttataacctg atgccataaa
     2101 gtcttttgcg gctttcgagg ctccggcatc agcggtagtc tgatatcctg tcagcaccca
     2161 ttttgtggca tcatccgttt tttccgtgct gattactggc gtaacgttac tgaaaccaat
     2221 ctgctgcgta ccggcagtga acgttttctc atcactccct ttcggtgcgg taattaacgt
     2281 cacgctcatt tcactggctg gtttgtttaa aaagtcaacc aggacgctat tattactgcc
     2341 tgacagtttg tctgtgacat tcaactggtc agcccggctg ttgttggtat gcatcaggaa
     2401 cgtggtatta tctgcagaaa gctcttttac ttgtaaagtg gcaaagtgtt tcccgtcccc
     2461 cagttcaatt cgcgagccct tagccttcag tgcaccggcc tctgattttc cgtcagagaa
     2521 ccacagagca ttgatcatat ttaccgtact ctgtgccccc gtaatactgc ctctcagaac
     2581 agcgttatat cccttcatta aggagctgaa taacggagag tccgtcttcc ctgcatcagc
     2641 atcactgtct cccagattaa tggttgccgc agtggttcct gcatgaatat ccgaactcag
     2701 atagcccata tttttaacag aaaagctggc gttatcttcg aggttaatcc cttctgttgt
     2761 agaaattacg ggtgagtaat acccctgttt ctgcgcagaa ggcatccccg tcagggtcag
     2821 agaagcattt tcttttacat ccacagcccc ggtgctgaaa ataccgctct gagccgtaag
     2881 ttttgctcct tttccgaggg tcaggctgct gttgacaaag ctgccaacac ggtcaaaaac
     2941 agcattctga gaggtcacac tcacggtgct atcataagcc tcaataccac cggtgaatct
     3001 ctccatgatt tgcagggatg atttattttc cagcgtaaca tttccgctgt aatcactctg
     3061 gtcaccaact gtggttgcag tggcactccc ttcaacagag gagaccgcat taccggttcc
     3121 gtcactcctg tcagtaaaga cacggctact tcccagaatg actgtacttc cattggtggc
     3181 gttaatatct ccctgcatct tgccgtttct ggacagatgg aatgttgccg catcgagttt
     3241 cagtttttcc agcgtaaact gacgggtttc ccagtcgctc tggctggaag aagtcgtcgt
     3301 tccagcatga ataactggat gcccctggaa tatcagcgtg ccggacttct tggttatcga
     3361 accttctggc aggttaactg aaccatcaag agccagtaca tccttacctg agagctgcgg
     3421 aatattcaca tccatattgc cgtttagttg gccgtgatac atataaggct ggctgctggc
     3481 ctcaattttt tgttgcttca cagtatcaat ggctttatta cgatcttttc cgacattctg
     3541 ccagacactg ctgttgttca gatcggagaa gaacggggaa tatgaactcg ctttcaggat
     3601 gaaatattgc ttggtagaac tgtcataata taggtctccc ggcgctcctc tccccccaaa
     3661 aatactgaca gtattaaccg gtacattgat gtcactggct tttaatgtct gaaggtcgag
     3721 tgttaccgtt gattttttct cactgttatt ggtaatgatg gccccgtagt cagccgcctg
     3781 aagacgggta aaggtcagat tattaccatt cagttccaga cgtcctccac ggtatcccca
     3841 cgaaatatta tccggattaa cttgctgtga gtcagaaagc acaactgtcg gacgaccgct
     3901 ggcaatacca acagagctga aagcctgtac ctttccgtca gcatccgcct gctggttgag
     3961 aataacggta ccgtccccca ccttgagtcc gcctgcattc acaccggtcc cgtttactgt
     4021 cagggtacct tcgccagttt tatgcaggtt atcaccggca actccgttca cctgccacag
     4081 aacgtgggtt cctttatcgg tgataatccc gccccccatc catgtctggc cgttcagggc
     4141 ggacaccctg taatcgccgg caaactgcag atacccagct ccctgattaa cggaattctg
     4201 cagaacgacc tcgccattat tgccggtgaa caggaggttt tttcctgcgt tcagatcatt
     4261 tcctttcttt ccatgcatat cccaagtcgt gtttccctga gtcagtgtgc cggtgccatt
     4321 agccgcatca tatttccact gcagtactcc ttcaccagag gtgtaggcta tagttttgtc
     4381 aaaatcattt tgcggttgct gaccaaggaa atcctgagtg gtgacgaccc agttatttcc
     4441 atagttagtt cctgaggaaa gcacgccaat cagtacccat tttttttgca gggaatcata
     4501 agcaaacagg ggggagccgc tgtcccccat attcagataa ttagccagag gtccattcgc
     4561 gggattaaaa atgtcaccgg tctgagctgt aatcatctgt ccgttgttat aggagttcag
     4621 cgcgctgacg gttcccccaa caagaaatcc gccattggta tacacttggg tgcgattacc
     4681 atttttgtct ttgacgtact gcatcccccc cccaaggcga tagaattcag taaagcgtcc
     4741 gccggcctga taggcaccgc tcacagcccc gatgtcagac acttctgccg gcgccacctc
     4801 tgtcaccagt ttactcaggc ggcgggtttt aatatcaaga ccgctattat tatttgtacc
     4861 aaccgcagta taggtgtttt tggcgtatcc gaagctcatc gtgtcagaac cgctgacatg
     4921 tttggcggta accacatact gaggggagta aagtgtatgg tctcccggag gcaggctacc
     4981 ggtagtaata gtggcactac tgaaatcagc catcggggct ttatcaagtt taccgaccag
     5041 attgccctgc ttgtcgtaaa tggaaatatt cgtggtcccc gacgtaaact gacctttatt
     5101 ctcggcaaag tcgcggaata tctgataagg gatctctgca ctgaccgttg ctgacagagc
     5161 tggccatgcc gaagaaagtg ataaagctgc aacagacgta agaataaccc gacgtgaaag
     5221 tctgcgatgg cttttgcagg taaccctgcg ggccagttcg gagacagcaa tcaggctttt
     5281 ggttatatgg caatacttaa gataatatat tttgttcata tagaaacatc aacctaattt
     5341 tgacaaagat taattacata ggatgtagat ggagcagtcc gagattctac taatatcaat
     5401 tattaagcat ctgtttttta gttggtgtaa tataattttt attaaggtaa gcggaaaaac
     5461 aaccaaaaaa aacgcaagat gttatttttt tatcattttg agccagtttg gctgggggga
     5521 tgctcttctc ttaagcttct tgctcaactt taaaaatagt tatgccagtt cggtatcgat
     5581 aagggccagt ttggatatc
//
LOCUS       DADMGC010000194         5554 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.194, whole genome shotgun sequence.
ACCESSION   DADMGC010000194 DADMGC010000000
VERSION     DADMGC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.194"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..638
                     /locus_tag="JNZ73_003894"
     CDS             <1..638
                     /locus_tag="JNZ73_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733988.1"
                     /translation="GACIVLMELMMMVQLWWSVWLCWPVLSLLIVGRGCGGLGAITTG
                     KDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNAV
                     LDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALG
                     LSRSALVVAAWLLCYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(692..1297)
                     /gene="azoR"
                     /locus_tag="JNZ73_003895"
     CDS             complement(692..1297)
                     /gene="azoR"
                     /locus_tag="JNZ73_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAY8733989.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1498..5400
                     /gene="hrpA"
                     /locus_tag="JNZ73_003896"
     CDS             1498..5400
                     /gene="hrpA"
                     /locus_tag="JNZ73_003896"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HAY8733990.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLSFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRCIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANDGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1377 a         1356 c         1540 g         1281 t
ORIGIN      
        1 taggcgcgtg cattgtgttg atggagctaa tgatgatggt tcagttatgg tggtcagtct
       61 ggttatgttg gccagtatta tcgctactca ttgttggccg tgggtgcggt gggcttggcg
      121 cgataacaac agggaaagat agtcagggga aactcccgcc cgccgtttac tggctgacat
      181 tgccctggcg catcgggatg tggctatcta tgcgttggtt ttgtcgtcgc ctggagccgg
      241 tgagcaaaat gactgctggt gtttatttaa gggcgtttcc acgacatatt ccggcacaga
      301 atgcggttct ggacgtcacc tttgaattcc ctcgtggacg agcgacaaaa gatcgactct
      361 atttctgtgt accgatgttg gatctggtgg ttccggaaga gggggagctc cgacaggccg
      421 tggcgatgct ggaaacatta cgcgaagagc aaggcagcgt tctggtccat tgcgcattgg
      481 gattatcgcg cagtgcgctg gtagtggcgg catggctgtt atgttacgga cactgtaaaa
      541 cagttgatga agcgattagc tttattcgag ccagacgctc gcatattgtg cttaaggaag
      601 agcacaaagc gatgttgaaa ttatgggaaa acaggtaagt ggattgagat gtggactgaa
      661 tatctacagt ccacatcaag accgtgtccg gttatgcagc aacaatgctg tcgatggctg
      721 cttttgcgtc agactgtgct ttcgctgcca tttccggacc gtatgcgatc ccttcggcga
      781 agacaaattt cacatcggta atgccgataa agccgaggaa cgtggacaga tacggcgtca
      841 ccaggtccgt tggtccatct ttgtggatcc caccgcggct ggtaataacg atggcttttt
      901 tacccgttac cagaccttcc ggaccgttct cggtatagcg gaaagtaacg cctgcgcgtg
      961 caaccaggtc aaaatagttt ttcaactgag ttgagatgtt gaagttatac attggtgccg
     1021 caataacgat aacgtcgtgg gctttcagct cggcaatcag ctcatcggaa agcgccagag
     1081 cttcctgctg acgcggagtc agcggcgcat cgctcggacg cagagcgcca accagttcgc
     1141 catccagtac cggaatcgga tttgcagcta ggtcgcgaac ggtgatttca tcagcggagt
     1201 gcttttcgcg ccattgttca acaaaataat cggacaactg attagactga gagtaccctg
     1261 ccaggatgct ggatttaaga actaatacct tgctcatggt gtttccttat agatgtttga
     1321 atggacgatg ccccgttgct tgctgacact ttattcacaa tcctgccaca gagatagcgc
     1381 aataaatcga agtctatgtt cgaatttatt gaacaacgca tagaaagccg cgatgtggta
     1441 ctctatatct atcatttaaa agaaaattaa tcaggcagac tactgcccac taacgttatg
     1501 acagaacaac aaaaattgac ctttactgcc ttgcaacagc ggctggattc gctgatgctg
     1561 cgcgacagac tgagtttttc tcgccgtctg cattgtgtta agaaggttaa aaatcctgat
     1621 gcacaacagg ccattttcca ggagatggcg aaagagattg accaggcggc agggaaagtc
     1681 ctgctgcgtg aagcggcacg accggaaatc acttatcctg acaatttacc ggttagtcag
     1741 aaaaaacagg acatactcga agcgattcgt gatcaccagg tggtgatcgt cgccggggaa
     1801 accggctccg gtaaaacgac tcagctaccg aaaatctgta tggagctggg gcgcggtatt
     1861 aaagggctga tcggtcatac ccagccgcgt cgcctggcgg cacgaacggt ggccaaccgt
     1921 attgcggaag agctgaaaac ggagccgggc ggttgcatcg gttacaaagt gcgtttcagc
     1981 gatcacgtaa gtgataacac gatggtcaag ctgatgaccg acgggatcct gctggcggag
     2041 atccagcaag accgcctgct gatgcagtac gacaccatca ttattgacga agcgcacgaa
     2101 cgcagcctga atatcgattt tctgctcggc tatttgaaag agttgctgcc gcggcgtcct
     2161 gacctaaaaa tcattatcac ttccgcgact atcgacccgg aacgcttttc gcgccacttt
     2221 aataatgcgc cgattattga agtctccggt cggacctatc cggtggaagt gcgctatcgc
     2281 ccgattgttg aagaagccga tgacaccgag cgcgatcagt tgcaggcgat ttttgacgcc
     2341 gtagacgaac taagccagga aagccccggc gacattctga tctttatgag cggcgagcgg
     2401 gaaattcgcg ataccgccga tgcgctgaac aagctgaact tacgccatac cgaaatcttg
     2461 ccgctttatg cgcggctttc gaacagcgaa cagaaccgcg tgttccagtc gcacagcgga
     2521 cggtgcattg tgctggcgac caacgtcgcg gaaacctcgc tgaccgtacc ggggattaaa
     2581 tacgtcatcg accccggtac agcgcgtatc agccgctaca gctatcgcac caaagtgcag
     2641 cgtttgccga ttgaacccat ttcgcaggcg tctgccaatc agcgtaaagg ccgctgtggt
     2701 cgtgtgtccg aagggatctg tattcgtctc tattccgaag acgatttcct ctcgcgcccg
     2761 gagtttaccg atccggagat tctgcgtacc aacctggcct cggttatttt gcagatgacc
     2821 gcgctggggc tgggcgatat cgctgcgttc ccgtttgtcg aagcaccgga taaacgcaat
     2881 atccaggatg gcgtgcgtct gctcgaagaa ctgggcgcga tcaccacgga tgaacaggcc
     2941 agcgcctata aactgacgcc gctcggtcgc cagctctcgc agttgcctgt cgacccacgt
     3001 ctggcgcgta tggtgctgga agcgcaaaaa catggctgcg tgcgtgaggc gatgattatc
     3061 acgtccgcgc tctccatcca ggatccgcgc gaacgtccga tggacaaaca gcaggcatcg
     3121 gacgaaaaac atcgtcgctt ccacgacaaa gaatccgact tcctcgcgtt tgtaaatctg
     3181 tggaattatc ttggcgagca gcaaaaggcg ctttcttcca acgccttccg tcgcctgtgt
     3241 cgtaccgatt acctcaacta tctgcgcgtg cgcgaatggc aggatatcta cacccagttg
     3301 cgccaggtgg tgaaagaact tggcattccg gtcaacagcg aaccggcgga gtatcgtgaa
     3361 attcacatcg ccttactgac cggtttgctt tcccatatcg gcatgaaaga tgccgataaa
     3421 caggaatata ccggtgcacg taacgcgcgt ttctccatct tccccggttc tggtttattc
     3481 aaaaagccgc cgaaatgggt aatggtggcg gaactggtag aaaccagccg cctgtggggg
     3541 cgcattgctg cgcgtatcga cccggaatgg gtagaacccg ttgctcagca tttgattaaa
     3601 cgcacctaca gcgaaccgca ctgggaacgg gcgcagggcg cggtgatggc aacggaaaaa
     3661 gtcactgttt atggtttgcc gattgttgct gcgcgcaagg tcaactacag ccagatcgat
     3721 tcggcgttat gtcgtgaact ctttattcgc cacgctctgg tggaaggtga ctggcagacg
     3781 cgtcacgcat tcttccgtga aaacctgaaa ctgcgggccg aagtggaaga gctggaacac
     3841 aaatcacgtc gccgcgatat tctggttgat gacgaaacgc tgtttgagtt ctacgaccag
     3901 cgcatcagcc acgatgtaat ctccgctcgt cacttcgata gctggtggaa aaaagtcagc
     3961 cgcgaaacgc ctgatttgct caactttgaa aagagcatgt tgatcaaaga aggggcggaa
     4021 aaaatcagca agctggatta cccgaacttc tggcatcagg gcaatctcaa gctgcgtttg
     4081 agctatcagt ttgagccagg cgcggacgcg gacggtgtga ccgtacatat tccgctgccg
     4141 ttacttaacc aggttgagga aagcgggttt gaatggcaga tccccggtct gcgccgcgaa
     4201 ttggtgattg ctctgattaa atcgttgccg aaaccggtac gccgtaattt tgtgcctgca
     4261 ccaaactacg ccgaagcgtt tttaggccgc gtcacaccgc tggagttacc gttgctcgac
     4321 agccttgagc gcgagttacg gcggatgacc ggcgttaccg ttgaccgcga agactggcac
     4381 tgggatcagg tgcccgatca cctgaaaatc accttccgcg tggtggatga caaaaacaag
     4441 aagctaaaag aagggcgctc gctacaagat ctgaaagatg cgctgaaagg caaagtgcag
     4501 gaaacgctgt ccgcggtggc ggatgacggt atcgagcaga gcggcttaca tatctggagt
     4561 tttggtcagc tgccggaaag ttacgaacag aagcgtggca actataaagt gaaggcgtgg
     4621 ccggcgctgg tggatgaacg cgacagcgtg gcgatcaaac tgtttgataa cccgctggag
     4681 caaaaacagg caatgtggaa tggtcttcgc cgtctactgc tgctaaatat ttcctcgccg
     4741 atcaagtatt tacatgagaa gttaccaaac aaagccaagc tggggctgta ctttaacccg
     4801 tatggcaaag tgctggagct gatcgacgac tgtatctcct gcggtgtgga tcaactgatc
     4861 gacgccaatg atggcccggt ctggacggaa gaaggctttg ctgcgctgca tgaaaaagtg
     4921 cgcgccgaac tgaatgacac ggtggtggat attgcgaagc aggtcgagca aatcctcact
     4981 gcggtgttca atatcaacaa acgtctgaaa gggcgggtgg atatgaccat ggcactgggg
     5041 ctttctgaca ttaaagcgca gatgggcggg ttggtatatc gcggttttgt cactggtaac
     5101 ggcttcaaac gtctgggcga cacgctgcga tatttgcagg cgattgaaaa acgactggaa
     5161 aaactggcgg ttgatccaca tcgcgatcgt gcacagatgc tgaaagtcga aaacgtccag
     5221 caggcgtggc agcaatggat caacaaactg ccgcctgcac gtcgtgagga tgaagacgtg
     5281 aaagagatcc gttggatgat agaagagctg cgcgttagtt acttcgctca acaacttggt
     5341 acgccttatc cgatttcaga taagcgtatt ttgcaggcga tggagcagat tagcggttaa
     5401 ccctgctatt tgcctaataa atctaaaacc cggtaagcat ttagcgccgg gttttttatt
     5461 aattctaaaa cggtaaggac aaaaattcag gaattcagaa aaatacaatt ctcagctgca
     5521 agatgaataa tgtttatcta cagcatttcc ttaa
//
LOCUS       DADMGC010000195         5537 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.195, whole genome shotgun sequence.
ACCESSION   DADMGC010000195 DADMGC010000000
VERSION     DADMGC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.195"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="JNZ73_003897"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="JNZ73_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HAY8733991.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="JNZ73_003898"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="JNZ73_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HAY8733992.1"
                     /translation="MTYVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..>5537)
                     /locus_tag="JNZ73_003899"
     CDS             complement(2659..>5537)
                     /locus_tag="JNZ73_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HAY8733993.1"
                     /translation="STSDAAGNASITLTFETGTSPDIAQVQVQNKLQLAMPSLPEAVQ
                     QQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVASNIKDPLSRTAGVGSVQLFGSE
                     YAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQLGGMPQVADQQLNASIIVQTR
                     LQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYSTVARYNGKPAAGIAIKLAAGA
                     NALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIEISIQEVFKTLVEAIILVFLVM
                     YLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINTLTMFGMVLAIGLLVDDAIVVV
                     ENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLSAVFMPMAFMSGATGEIYRQFS
                     ITLISSMRLSVFVAMSLTPALCATILKAAPEGGHKPNALFARFNTLFEKSTQHYTDST
                     RSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEEDQGVFMTTAQLPSGATMVNTTK
                     VLQQVTDYYLTKEKDNVQSVFTVGSFGFSGQGQNNGLAFISLKPWSERVGEENSVTAI
                     IQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMELLDNGNLGHEKLTQARNELLSL
                     AAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVALSDINQTISTAFGSSYVNDFL
                     NQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAPLSAYSSTEWTYGSPRLERYNG
                     IPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYSWTGLSYQEALSSNQAPALYAI
                     SLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLATDLRGLSNDVYFQVGLLTTIGL
                     SAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRPILMTSLAFILGVLPLVISHGA
                     GSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHLFARFKKA"
BASE COUNT         1463 a         1288 c         1302 g         1484 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atatcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaaaaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg acttatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggctgt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag tccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccaactc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagctg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccac
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaag
//
LOCUS       DADMGC010000196         5531 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.196, whole genome shotgun sequence.
ACCESSION   DADMGC010000196 DADMGC010000000
VERSION     DADMGC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.196"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            100..1008
                     /locus_tag="JNZ73_003900"
     CDS             100..1008
                     /locus_tag="JNZ73_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAY8733994.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFY
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAADKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPETMIPGSTVPFISTK"
     gene            complement(1054..3045)
                     /gene="tkt"
                     /locus_tag="JNZ73_003901"
     CDS             complement(1054..3045)
                     /gene="tkt"
                     /locus_tag="JNZ73_003901"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY8733995.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3323..4081
                     /locus_tag="JNZ73_003902"
     CDS             3323..4081
                     /locus_tag="JNZ73_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY8733996.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4287..5207)
                     /gene="speB"
                     /locus_tag="JNZ73_003903"
     CDS             complement(4287..5207)
                     /gene="speB"
                     /locus_tag="JNZ73_003903"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAY8733997.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5343..>5531)
                     /locus_tag="JNZ73_003904"
     CDS             complement(5343..>5531)
                     /locus_tag="JNZ73_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8733998.1"
                     /translation="FQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALPPCS
                     GQAFPADNNGYAPPPCRK"
BASE COUNT         1325 a         1482 c         1478 g         1246 t
ORIGIN      
        1 ttcgctatac ccctttatag attctgaatt ctcacctatc ggctgttttc tcctcatctc
       61 atgtgacata ttcccatact ttactcaagg aagattgtca tgaaagcacc tgtttcatta
      121 acctcatttc gcccacaaaa atctcttgct atcgccattg gcgtgcttgc cgtggttgtg
      181 ctgccatttc tctcgtatta caccgttaat gaaggtgaac gcggcatttt gctgcgctac
      241 ggaaaaatcg taaaagttgc cgagccgggg ctgggtttta agatcccttt tatggaaagc
      301 gtggagaaaa tttccacgcg taatcaggct gtggtctatc agggattaca ggcctacagc
      361 cgcgaccagc aaccggcaca aatgacagta tcagtcagct tctacattaa accttcagaa
      421 gcaggcgcgg tttataccac ctacaacact attgaagcat taaaagatcg tctaattgtc
      481 cgtcagttgc caactcagct ggagaacatt ttcggccaat ataccgccat cagcgccgta
      541 caggatcgta caaaattggt tcaggatctg caaaatgcca tgcgcaaagc cgttgttggg
      601 ccagtggtaa ttgacggggt ccagattgaa aatattgatt tttctgacgc ttatgaaaag
      661 tcgattgaaa accgaatgaa agcagaagtc gccattgcca cgcgtaagca aaatctggaa
      721 acagagaaaa tccaggcgca aatagcggta actcaggccc aggcagaagc agacagtaaa
      781 ctggcggcag acaaagcaga agcggaaact attcgcgtaa gaggcgcggc agaggctgaa
      841 actattcgtt taaaaagtgc ggctgaagca gaagcaatcc gcctgcgcgg tgaagctctg
      901 cgggataatc ccggacttgt agccctcacc actgccgaac gttgggacgg taaactgccc
      961 gaaaccatga tccccggcag cacagttccg tttatctcta ctaagtaaaa aaagggccgc
     1021 tttcgcggcc cttctcacaa aacgtcttac tgattacagc agcgcttttg ctttcgcaac
     1081 aacgttatca acagtgaagc cgaactcttc aaacagcagc tctgccggag cagattcacc
     1141 gaaggtggtc atgccgacga tagcgccgtt caggccaaca tacttgtacc agtagtcagc
     1201 aatacccgct tctaccgcaa cgcgtgcagt aaccgctttc ggcagtacgg attcacggta
     1261 agccgcatcc tgcttgtcaa atgcgtcggt agacggcatg gacaccacgc gcgctttcac
     1321 gccttcggca gtcagttttt cgtaggcagc aaccgccagt tcaacttcag aaccggtggc
     1381 gatgaagatc agttccggct gaccagcaca gtctttcagc acataaccac cgcgcgcgat
     1441 gtttgccagc tgctcttcag tacgttcctg ctgcgccagg ttctgacggg agaggataag
     1501 cgcagtcggg ccgtcctgac gctcaacgcc gtatttccac gcgaccgcgg attcaacctg
     1561 gtcacacgga cgccatgtag acatgttcgg ggtcacgcgc agagaagcga cctgctctac
     1621 cggctggtga gtcgggccat cttcgcccag accgatggag tcgtgggtgt aaaccatcac
     1681 ctggcgctgt ttcatcagcg cagccatacg tacggcgtta cgtgcgtatt ccacgaacat
     1741 caggaaggtg gaggtgtacg gcaggaagcc accgtgcagg gagataccgt tagcaatcgc
     1801 ggtcataccg aactcgcgaa caccgtagtg gatgtagtta cctgcagcat cttcgttgat
     1861 tgctttagaa ccagaccaca gggtcaggtt agacggtgcc aggtcagcag agccgccgag
     1921 gaattcaggc aacagcgggc cgaacgcttc gatagcattc tgcgacgctt tacggctggc
     1981 gattttcgcc ggattagcct gcagtttagc gataaactct ttcgctttgg cgtcgaagtc
     2041 agacggcatt tcgcctttca tacggcgggt aaattcagcc gcttcctgcg gataagcttt
     2101 cgcgtaagcc gcaaacttct cattccatgc agattctttc gcctggcctg cttctttcgc
     2161 atcccactga gcatagattt cagacgggat ttcgaacggt gcatatttcc agcccagctg
     2221 ttcgcgggtc agggcaattt cagcgtcgcc cagcggcgca ccgtgggagt cgtgagtacc
     2281 ggctttgttc ggggaaccga aaccgatgat ggttttgcac atcagcaggg acggtttgtc
     2341 agtcactgcg cgcgcttctt caactgcgcg tttgatggat gccgcgtcat gaccgtcgat
     2401 gtcgcgaata acgtgccagc cgtaagcttc gaaacgcatt gctgtgtcgt cggtgaacca
     2461 gccttcaacg tgaccatcga tagaaatacc gttgtcatcg tagaacgcaa tcagtttacc
     2521 cagcttcagc gtacccgcca gagagcaaac ttcgtgggag atgccttcca tcatgcagcc
     2581 gtcgcccatg aaggcgtagg tgtagtggtc gacaatgtcg tggcccggac ggttaaactg
     2641 cgccgccagc gttttttctg caatcgccat accgactgcg ttggcaatac cctgacccag
     2701 cggaccggtg gtggtttcca caccagcggt ataacctact tccgggtggc ccggagtttt
     2761 agagtgcagc tgacggaagt ttttcagttc ttccatcggc agatcgtaac cggtgaggtg
     2821 cagcaggctg tagatcagca tggagccgtg gccgttggac agcacgaagc ggtcacggtc
     2881 agcccaggac ggattctgcg ggttgtgttt caggaaatca cgccacagga cttcggcaat
     2941 gtcagccata cccatagggg cacccgggtg accggatttg gctttctgta ctgcgtccat
     3001 gctcagcgca cgaatagcat tggcaagctc tttacgtgag gacattttga ctccagatcg
     3061 gatgatgaag ggcacgccct taacgacttg acgacagcgc gttttgggct acgccggaaa
     3121 atttgccaac aatttaccgc aagccgcgcg tcatgtacat ggaacatcct tttgccgctt
     3181 cagaaatctc tggatcatgc tcgcatgttg cgcaatctgc tcgcccgtcc gctgcgcttt
     3241 tccttatact gagactgagc gtcgattcac ctgcaaacgg cgcattttta gaataatcct
     3301 gaccttgtgc ggaagagaaa acatgaaaat tcgcgcctta ttggtagcaa tgagcgtggc
     3361 aacggtactg accggttgcc agaatatgga ctccaacgga ctgctctcat caggagcgga
     3421 agcttttcag gcttacagtt tgagtgatgc gcaggtgaaa accctgagcg atcaggcatg
     3481 tcaggagatg gacagcaagg cgacgattgc gccagccaat agcgaatacg ctaaacgtct
     3541 gacaactatt gccaatgcgc taggcaacaa tatcaacggt cagccggtga attacaaagt
     3601 gtatatggcg aaggatgtga acgcctttgc aatggctaac ggctgtatcc gcgtctatag
     3661 cgggctgatg gatatgatga cagataacga agtcgaagcg gtgatcggtc acgagatggg
     3721 gcacgtggcg ttaggccatg tgaagaaagg gatgcaggtg gcgcttggta caaatgccgt
     3781 gcgagtagct gcggcctctg cgggcgggat tgtcggcagt ttatctcaat cacaacttgg
     3841 tgatctgggc gagaaattag tcaattcgca attctcccag cgccaggaag cagaagccga
     3901 tgattattct tacgatcttc tgcaccaacg cggcatcagc ccggcaggtc ttgccaccag
     3961 ctttgaaaaa ctggcgaagc tggaagaagg ccgtcaaagc tcaatgtttg acgaccatcc
     4021 tgcatccgcc gaacgtgccc agcatattcg cgatcgcatc agcgcggatg ggattaagta
     4081 aagcctggtg gtgtcgaacg taggtcagat aaggcgttca cgccgcatct gacattaatg
     4141 gcacatttgt taccttgtgc gcattgccgg atgcgatgct ggcgcatctt atccggccta
     4201 cgggtgccga acgtaggtcg gataaggcgt tcacgccgca tccgacatta atggcacgtt
     4261 ttacccgtgc gcatcgcatc tggtgcttac tcgccctttt tcgccgcctg aatatacagc
     4321 atctccagcg ccagcgtcgc cgcagccaga gcggtgattt ccgactgatc gtatgccgga
     4381 gccacttcca ctacgtccat cccaacgatg ttgagatctt tcaggccgcg taccagttta
     4441 atggcgcgat cggaggtcag gccgccaatc actggcgtac cggttcccgg cgcgaaagca
     4501 ggatccaggc agtcgatatc aaaagtcagg taaaccggca tatcacccac aatctgtttc
     4561 acctgggcga taacgtcatc cacgctgcga tcgttcacct ggcaggcgtc cagcacggta
     4621 aagccgttgt ctttatcaaa ctcggtacga ataccaatct gcacggaatg attcgggtcg
     4681 atcagacctt ctttcggcgc ggtatagaac atagtgccgt ggtcaaattc acaaccgttc
     4741 gcataggtat cggtgtgggc gtcaaagtgt accagcgcca ttttgccgaa atgcttcgca
     4801 tgagcacgca gcagcggcag cgtaacaaag tggtcaccac cgaaggagag catacgctta
     4861 ccggcagcca gcagcttctc ggcgtgcgcc tgcagctttt cgctcatctc acgggcatcg
     4921 ccaaaggcat ataccagatc gccgcagtcc acgacgttca gacgctcacg catgtcgaaa
     4981 ttccacggga agcggttgtg ttcccaggcc agattcgtcg aaacctgacg gatcgctgcc
     5041 ggaccgtggc gaccacccgc acgaccagaa gtggccatat cgaacggcac gccagtaatc
     5101 acccagtctg catcgctgtc atacggctgg aagttcatcg gcaggcgtaa aaaaccaaag
     5161 gcattggaaa ccagtgagtt atcgtattga tgacctaagg tgctcatgga tggactccta
     5221 ttacaaagtc gatataaaaa aacccttccg cgtcgttagg cccgacgagg aagggttgga
     5281 tttgcgaaac acaagtttgt gctaattatc gccttaaatg ggcgaggcat caagcgaggt
     5341 tgctatttcc gacacggtgg cggcgcataa ccattgttat ctgccggaaa ggcttgtcca
     5401 gagcacggcg gcagcgcgtt ttgcgccggg ccttgtaatt caaaggcggc aaagccgatt
     5461 accccggtat tacgcacatc gccttgcgcc gaattggctg cgtaggaatc agcaacgttg
     5521 gcgaactgaa a
//
LOCUS       DADMGC010000197         5465 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.197, whole genome shotgun sequence.
ACCESSION   DADMGC010000197 DADMGC010000000
VERSION     DADMGC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.197"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(296..580)
                     /locus_tag="JNZ73_003905"
     CDS             complement(296..580)
                     /locus_tag="JNZ73_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAY8733999.1"
                     /translation="MLDMAGLRADILSPVKETGTLLGVVSSQAAELCGLKAGTPVVVG
                     GGDMQLGCLGLGVVRPAQTAVLGGTFTDNKQCTLSEARHQLPITPFRMPS"
     gene            614..1420
                     /locus_tag="JNZ73_003906"
                     /pseudo
     CDS             614..1420
                     /locus_tag="JNZ73_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            1432..2455
                     /gene="lsrB"
                     /locus_tag="JNZ73_003907"
                     /pseudo
     CDS             1432..2455
                     /gene="lsrB"
                     /locus_tag="JNZ73_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            2482..3357
                     /gene="lsrF"
                     /locus_tag="JNZ73_003908"
     CDS             2482..3357
                     /gene="lsrF"
                     /locus_tag="JNZ73_003908"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HAY8734000.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSWIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PGVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            3381..3671
                     /gene="lsrG"
                     /locus_tag="JNZ73_003909"
     CDS             3381..3671
                     /gene="lsrG"
                     /locus_tag="JNZ73_003909"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HAY8734001.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            3710..4468
                     /gene="tam"
                     /locus_tag="JNZ73_003910"
     CDS             3710..4468
                     /gene="tam"
                     /locus_tag="JNZ73_003910"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HAY8734002.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(4472..5386)
                     /locus_tag="JNZ73_003911"
     CDS             complement(4472..5386)
                     /locus_tag="JNZ73_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734003.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNELPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT         1379 a         1306 c         1457 g         1323 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgcagtaaca ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcatg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagatttcgt ttggttcgac catcggcctc tgcgccattg cattgggcgt
      661 actgtttcaa agtggtgtgc cgatgccgct ggcgatactc ctgaccttac tgctcggcgc
      721 attgtgcggg ctgatcaacg ccggattaat tatctatacc aaagttaacc cgctggtgat
      781 tacgcttggc acgctgtatc tgtttgccgg aagcgctctg ctgctttccg gtatggccgg
      841 agcgacgggg tacgaaggta ttggtggatt cccgatggcg tttacagatt tcgctaacct
      901 ggatgtgctg ggactccccg ttccgctgat tatcttcctg atatgtctcc tcgttttctg
      961 gctctggctg cataaaaccc atgccggacg taatgtgttt ttgattgggc aaagcccgcg
     1021 cgtggcgctt tatagcgcga ttccagttaa ccgcacctta tgtgcgctct atgccatgac
     1081 ggggctggcg tctgcggtcg ccgctgtgct gctggtatcg tattttggtt cagcacgttc
     1141 cgatctcggt gcgtcgtttc tgatgcccgc catcaccgcc gtggtgcttg gcggggccaa
     1201 tatttatggt ggttccggtt ccattatcgg caccgccatt gcggttttat tagtgggata
     1261 tttgcagcaa ggtttgcaaa tggcaggagt gccaaatcag gtgtccagcg ccctttccgg
     1321 tgcgctgctt atcgtcgttg tcgtaggtcg ttccgttagc ctgcatcgcc agcaaattaa
     1381 agagtggctg gcgcgtcggg ccaataaccc attgccataa aggatatctt catgacactt
     1441 catcgcttta agaaaatcgc cttacttagc gctcttggca ttgccgcaat ctctatgaat
     1501 gtgcaggccg cagagcgtat tgcatttatt cccaaactgg ttggcgtggg attttttacc
     1561 agcggtggca acggcgcaca acaagcgggt aaagagctga gcgttgatgt gacctacgac
     1621 gggccgacag aacccagtgt ttctggtcag gtacagttga ttaataactt cgtcaatcaa
     1681 ggttataacg ccattattcg tttctgcggt ttcgcctgat ggcttgtgtc cggcactgaa
     1741 acgcgccatg caacgtggtg tgagagtgct gacctgggac tctgatacta aaccggagtg
     1801 ccgctcttac tacattaatc agggaacgcc cgcccagttg ggaggtatgt tggtggatat
     1861 ggcggcgcgt caggtgaata aagacaaagc caaagtcgcg tttttctact caagccccac
     1921 cgttacggac caaaaccagt gggtgaaaga agcgaaagcg aaaatcgcca aagagcatcc
     1981 aggctgggaa attgtcacta cgcagtttgg ctataacgat gccactaaat cgttacaaac
     2041 cgcagaagga atattaaaag cgtatagcga tctcgacgcc attatcgccc ccgatgccaa
     2101 cgccctgccc gctgccgcac aagccgcaga aaacctgaaa aatgacaaag tagcgattgt
     2161 cggattcagt acgccaaatg tgatgcgccc gtatgtagag cgcggcacgg tgaaagaatt
     2221 tggactgtgg gatgtggttc agcaaggcaa aatttcagtg tatgtcgcgg atgcattatt
     2281 gaaaaaagga tcaatgaaaa cgggcgacaa gctggatatc cagggcgtag gtcaggttga
     2341 agtctcgcca aacagcgttc agggctatga ctacgaagcg gatggtaatg gcatcgtact
     2401 gttaccggag cgcgtgatat tcaacaaaga gaatatcggc aaatacgatt tctgatgtgc
     2461 attacttaac cggagtaagt tatggcagat ttagacgata ttaaagatgg taaagatttt
     2521 cgtaccgatc aaccgcaaca aaatatccct tttaccctga aaggttgcgg tgcgctggat
     2581 tggggaatgc agtcacgctt atcgtggata tttaatccga aaacgggtaa aactgtgatg
     2641 ctggcctttg accatggtta ttttcagggg ccgactaccg gacttgaacg cattgatata
     2701 aatatcgccc cgctgtttga acatgccgat gtattaatgt gtacgcgcgg cattttgcgc
     2761 agcgtagttc ccccggcgac caataagccg ggggtactgc gggcgtcagg tgcgaactct
     2821 attctggcgg aattaagtaa tgaagccgtg gcgttatcga tggatgatgc cgtgcgcctg
     2881 aacagttgcg cggtggcggc gcaggtttat atcggcagcg aatatgaaca tcagtcgatc
     2941 aaaaatatta ttcagctggt cgatgccgga atgaaagtgg gaatgccgac catggccgtg
     3001 actggcgtgg gcaaagatat ggtgcgcgat cagcgttatt tctcgctcgc gactcgaatc
     3061 gccgctgaaa tgggggcgca aattatcaaa acctattatg tcgaaaaagg ttttgaacgg
     3121 attgttgccg gatgtccggt acccattgtt attgctggcg gtaaaaaatt accggagcgc
     3181 gaggcgctgg aaatgtgctg gcaggctatc gatcagggcg cttctggtgt ggatatgggg
     3241 cgtaatattt tccagtctga ccatccggtg gcgatgatga aagccgtaca ggcggtggtt
     3301 caccataacg aaacggctga tcgggcatat gaactctatc tgagtgaaaa acagtaactg
     3361 cggatttaag gagaagaatt atgcacgtca cactggttga aattaacgtt catgaagaca
     3421 aggttgacga gtttatcgaa gtttttcgcc agaaccacct gggctctgta caggaagaag
     3481 gtaatttgcg cttcgatgtc ttacaggacc cggaagtgaa ttcgcgcttt tatatttacg
     3541 aagcctataa agatgaagac acagtggcat tccataaaac cacgcctcac tacaaaacct
     3601 gtgtcgcgaa actggaatct ttaatgaccg ggccgcgtaa aaaacgtctg ttcaatgggt
     3661 tgatgccgtg aggctacaat tggggtaacg cgctgacggg agtaaaaaaa tgtctgactg
     3721 gaacccctct ttatatctgc acttttccgc tgaacgatcg cgtccggcgg tggagctgct
     3781 tgccagagtg ccgctggaga atgtcgaata tgttgccgat cttggctgtg gcccaggtaa
     3841 cagcaccgcc ctgctgcatc aacgttggcc tgcggccagg ataacaggca tcgactcgtc
     3901 tccggcgatg attgctgaag cgcgcagtgc tttgccagac tgccagttcg tggaagcaga
     3961 catccgcaac tggcaaccgg aacaggcact cgatctgatt tttgctaatg cctcactgca
     4021 atggctgccc gaccactacg aattgtttcc tcacttggtt tctttactta atccgcaggg
     4081 cgtgctggca gtacagatgc cagataactg gctggagccg acccatgtgc tcatgcgcga
     4141 agttgcttgg gaacaaaact acccggatcg cgggcgtgag ccgttggctg gcgttcatgc
     4201 ttactacgat attttgagcg aagccggatg tgaggtcgat atctggcgaa ccacttacta
     4261 tcaccagatg ccgtcgcacc aggcgattat cgattgggtg accgccactg gattacgtcc
     4321 gtggttacag gatctgaccg agagcgaaca gcagcttttt cttacgcgct accatcagat
     4381 gctggaagag cagtatccac tgcaagagaa cggacagata ctgctggcat ttccgcgtct
     4441 gtttattgtt gcccgccgta cggagtaatt atcacgtcag ctggtaatga cgatcgggaa
     4501 gaatttttgc tggaatttct gcttcatcgt tcatctgtaa caggtcaatt tcaatagcgt
     4561 tgcagatggc atccagcggt aactcattgt tttcagtacc gaacggatct tccagttctt
     4621 ccgccagaca atccagcgaa ataaaagtgt aggaaatcag cacagagata aaaggcgtca
     4681 tgtaatgcag gtccacgacc agcgcgaacg gcagcatgat acaaaacaga taaacggtac
     4741 gatgcaaaat cagggtgtag gcaaagggaa ttggcgtgta ggcaatgcgc tcacagcctg
     4801 ccagaaccgc tgaaatatca ttaagccgat cgttgaggct aataaacagg atatctgaaa
     4861 gctgtccatt gcggcgctga accgccaacc attctcccat tattaacaag atacggttag
     4921 ctggagagtt cgaagccagt acacgctgaa gatctccagt cttgagataa tgagccagca
     4981 cttccgcctg tggctgtttg cgtaatgtca tgcgtaaaca gtgggcgaag gcgatttgca
     5041 gcctggcaaa ctcccttaca cttgccgaat ccggcaatgt cgtttttacc tctcgcagta
     5101 acgaccgtga ggcaatcatc aactgccccc aaagttttcg cgcttcaacg taacgggcgt
     5161 acccggcatt attacgaaaa ccaagaaaaa tggcgatggc gacaccgaga atgctgaacg
     5221 gtgcgagggt gaatttgatg cccagatgcg tgtaccaggg cagcatgaat ataacagcaa
     5281 tagaaaagag aaaattgagt agtaagcgcg aggatatctt ggataatact gagccgtgcc
     5341 agacaaaaat acggcgcagc cagtgttgtt gtggacgaac aatcatggtt atcttcaggc
     5401 gtggaaaagt cgccctatta aacgtgatta cgatcacatt ctcaagacgc tacttacaaa
     5461 ttacc
//
LOCUS       DADMGC010000198         5449 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.198, whole genome shotgun sequence.
ACCESSION   DADMGC010000198 DADMGC010000000
VERSION     DADMGC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.198"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..228
                     /locus_tag="JNZ73_003912"
     CDS             <1..228
                     /locus_tag="JNZ73_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAY8734004.1"
                     /translation="RATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESLRT
                     GETIRVSRLAEPFWQDHFLGARRILTEEMIL"
     gene            404..901
                     /locus_tag="JNZ73_003913"
     CDS             404..901
                     /locus_tag="JNZ73_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8734005.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTIQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(1118..2857)
                     /gene="flhA"
                     /locus_tag="JNZ73_003914"
     CDS             complement(1118..2857)
                     /gene="flhA"
                     /locus_tag="JNZ73_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HAY8734006.1"
                     /translation="MLSRSDLLTLLTINFIVVTKEAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLSGGVC
                     IGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDVRH
                     QLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEKSL
                     ETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVIS
                     DGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDGVL
                     GNDPAYGIPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQ
                     LHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSTVTKD
                     HILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQAGKVMLDSVP
                     VDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEV
                     PDELELKIMGALM"
     gene            2802..3587
                     /gene="lafU"
                     /locus_tag="JNZ73_003915"
     CDS             2802..3587
                     /gene="lafU"
                     /locus_tag="JNZ73_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAY8734007.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            3658..4713
                     /gene="dinB"
                     /locus_tag="JNZ73_003916"
     CDS             3658..4713
                     /gene="dinB"
                     /locus_tag="JNZ73_003916"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAY8734008.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            4765..5058
                     /gene="yafN"
                     /locus_tag="JNZ73_003917"
     CDS             4765..5058
                     /gene="yafN"
                     /locus_tag="JNZ73_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HAY8734009.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            5061..>5449
                     /gene="yafO"
                     /locus_tag="JNZ73_003918"
     CDS             5061..>5449
                     /gene="yafO"
                     /locus_tag="JNZ73_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HAY8734010.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFR"
BASE COUNT         1297 a         1398 c         1471 g         1283 t
ORIGIN      
        1 cgggcaacga ttgtggcgaa taacgacctg cgccggggag atttgctatt tttccatatc
       61 cacagccgcg agattgccga ccatatgggg gtgtatctgg gcgacggtca gtttattgag
      121 tcactgcgca ccggggaaac cattcgggta agccggttag ccgaaccttt ctggcaggac
      181 cattttttag gcgcacgcag gattttgacg gaagagatga ttttgtaggc cggataaggc
      241 gtttacgccg catccggcgg ttgtgcacgg atgcctgatg cgacgctgac gcgtcttatc
      301 atgcctacaa atctgtgcgt ttttgcgagc cgctttcccg atataacaca gcgtcattgt
      361 cggcctttcc ttttcctctt accgttgaga aaaaggagtc gctatgtcgg aatatcgtcg
      421 ttattacatc aaggggggaa catggttttt cacggtaaat ttacgcaatc gtcgaagcca
      481 tcttctgacc atccagttcc agacgctccg taacgccatt attaacgtta agcgagacag
      541 accttttgaa attaacgcct gggtcgtttt gccggagcat atgcattgca tatggacatt
      601 acccgaaagc gatgatgatt tttcctctcg ctggcgggag attaaaaagc agttcaccca
      661 tgcttgcgga ttgaaaaata tctggcaacc gcgtttttgg gagcatgcca ttcgcaatac
      721 aaaagattat cgccatcatg ttgattatat ttatataaat ccagagaaac atggctgggt
      781 aaagcaggtg agtgattggc cattctcaac gttccatcgc gatgtcgcgc gagggttata
      841 tcccatcgat tgggcgggag acataacgga tttaagtgcc ggggagcgta tcattttata
      901 actgtgcgct ggtgccggat gcgcttacgc ttatccggcc tacaaatcgt gctttaacgg
      961 taggccggat aaggcgttca cgccgcatcc ggcagttatg cgcagatgcc tgatgcgacg
     1021 ctggcgcgtc ttatcatgcc taaaaacttg tgcctgaacg gtaggccgga taaggcgttt
     1081 acgccgcatc cggcaattgt gcaccgatgc cggattctta catcaacgcc cccataatct
     1141 tcaactccag ctcatccggc acttcgttat acgacagcac atgcagcccc ggcgcaaaca
     1201 accttgcata acgcgccagc aaagggcgta gctgcggcgg caccagcagc accggatctt
     1261 tccccgccgc tttcatctgc tctttcacct gtggcatcgt gctctggaac tggttgagca
     1321 tattcgggtc aaccggcacg ctgtcgagca tcactttgcc ggcctgttgc gcctgattca
     1381 ctacgttggt cagcagattt tccagctcat tattcagcgt atacaccgtc agctcctgct
     1441 tgcgaacgaa cggatgagta atgctgcgcc gcagcgccag gcgcacatcg gccgccagca
     1501 gaatatgatc tttagtcacc gtgctactgg cgaccagcac ggtggcgatg gtgacgatat
     1561 cgcgcaggga aacgccttcg gtcagcagcg cccggtacac tttcagcaat tggctgtaat
     1621 tgagcgccgc gcttaaatcc tccgccaggc gcggtgccat cgacgacaaa cggttatgca
     1681 actgcgtgat gtcatcatag ttaaacaaat caggaatata gctgcgcaca atcttgttca
     1741 catgggtggc aatcacactg gcgctgtcga tcacctgata ccccatattc agagccttcg
     1801 ctttctgcgc aggctggatc caggtgaccg gtatcccgta cgccgggtcg ttccccagca
     1861 cgccgtcaat ctcgccgtag gtttcgctgg agggcagcgc catcagttta tccgccggaa
     1921 tatccgcttc atcagcctta atgccgttga tgaaaatagc gtactggctg ggcttgaggc
     1981 ggaagttttc ccgaatgcgg atctccggca gcagcacgcc gttaccgtcg gaaatcacct
     2041 gccgaacgcc gcgaatccgc tgggtgagcg ggttgccctg cgctttgtcc accagcgcca
     2101 ccagcttgta gccgaggctt aagctgatgg gctcgatcag cggaatagtt tcccaactga
     2161 cctgttgttc gctggtttca gtgatggtgc gggtcagcgt ttcgaggctt ttctcttccg
     2221 cctccgccgc ctgcgggcgt ttgctcatcc gccagccggt aaagccaagc agggcgctga
     2281 acagcaaaaa cggcagatgc ggcatccccg gcaccaccgc cagcacgaac atgatcccgg
     2341 tggcggtgta gagcaccgac gggcttgcca gcaactggtg gcgcacgtca tgggcgatat
     2401 cgccgttgtc gctgacgcgg gtgacgataa tcgccgccgc ggtggagagc agcagggaag
     2461 ggatctgcgc caccaggccg tcgccgatgg tcatcagcac gtattgctgg aaggcggcgt
     2521 cggcgctcag gttgtatttg aagatgccga tacagacgcc gccgctcagg ttgatcgcca
     2581 gaatcatcat cccggcgatg gcgtctccgc gtacaaactt cgatgccccg tccatcgcgc
     2641 cgtagaaatc ggcctcgctg gcaacatctt tacgccgggt ttgcgcctgc gcctggttga
     2701 tcaatccggc gttaagatcg gcgtcaatcg ccatctgttt gccgggcatc gcgtccaggg
     2761 taaagcgggc agaaacctcg gaaatacgct cggcctcttt ggtgacgacg ataaagttga
     2821 ttgtcaacag cgtcagtaaa tccgagcgtg aaagcattat cgccgcgctg cacgggcagt
     2881 cgatttttaa cggtggtggg ctgtcgccgt taaataaaat cagcccgtct catccgccaa
     2941 aacctgccac cgtggcggtg ccggaagaga cggagaaaaa agcgcgtgat gtgaatgaga
     3001 aaacggcgct gctgaagaag aaatccgcca ccgagcttgg cgagttggca accagtatca
     3061 acaccattgc ccgtgatgcg catatggaag cgaatctgga gatggagatt gttccccagg
     3121 gattacgcgt gctgattaaa gacgaccaga atcgcaatat gttcgaacgc ggcagcgcgc
     3181 agattatgcc gttctttaaa actttgctgg tggagctcgc gccagtgttc gactcgctct
     3241 ataacaaaat tattattacc gggcataccg atgcgatggc ctacaaaaac aatatctaca
     3301 acaactggaa cctttcgggc gatcgcgcgc tttcagctcg tcgggtgctg gaagaggccg
     3361 gaatgccgga agataaagtg atgcaggtaa gcgcaatggc ggaccagatg ctgctggacg
     3421 cgaagaatcc gcaaagcgcg ggcaatcggc gtattgagat tatggtactg accaaaagtg
     3481 cgtccgatac gctgtatcaa tactttggtc agcatgggga taaagtggtg cagccgctgg
     3541 tgcaaaagct ggataagcag caggtgcttt cgcagcgaat gcgctaaatg ctgaatcttt
     3601 acgcatttct caaaacctga aatcactgta tactttacca gtgttgagag gtgagcaatg
     3661 cgtaaaatca ttcatgtgga tatggactgc tttttcgcag cggtggagat gcgcgacaat
     3721 cccgctctgc gcgatatccc tattgctatt ggcggcagcc gcgaacgtcg gggggtgatc
     3781 agcaccgcca attatcccgc gcgtaaattt ggcgtacgta gcgctatgcc tactgggatg
     3841 gcgctcaaat tatgcccaca tctcaccttg cttccggggc gctttgacgc ctacaaagaa
     3901 gcctcaaatc atatccgtga aatcttctcg cgctacacct cgcgcattga accgttgtca
     3961 ctggatgagg cttatctcga tgttaccgat agcgtccatt gccacggttc tgcgaccctc
     4021 atcgcccagg aaatccgcca gacgatcttc aacgagctgc aactgacggc atctgcgggc
     4081 gtggcaccag taaagtttct cgccaaaatc gcctccgaca tgaataaacc caacggtcag
     4141 tttgtgatta cgccggcaga agttccggca tttttacaaa ccttaccact ggaaaaaatc
     4201 cccggcgtcg gcaaagtctc ggcggcaaaa ctggaagcga tggggctgcg gacctgcggt
     4261 gatgtacaaa agtgtgatct ggtgattctg cttaaacgct ttggcaaatt tggccgcatt
     4321 ttgtgggagc gtagtcaggg gattgacgag cgcgacgtta acagcgaacg gttacgaaaa
     4381 tccgtcggcg tggaacgcac gatggcggaa gatattcatc actggtctga atgtgaagcg
     4441 attatcgagc ggctgtatcc ggaacttgaa cgccgtctgg caaaggtaaa acctgattta
     4501 ctgattgctc gccagggggt gaaattaaag ttcgacgatt ttcagcagac cacccaggag
     4561 cacgtctggc cgcggctgaa taaagctgac ttaatcgcca ccgcgcgtaa aacctgggat
     4621 gaacgccgcg gcgggcgcgg tgtgcgtctg gtggggctgc atgtgacgtt gcttgacccg
     4681 caaatggaaa gacaactggt gctgggatta tgatgtatac tattatgtat attctggtgt
     4741 gcattattat gagggtatca ctgtatgcat cggattctcg ctgaaaaatc ggtcaatatc
     4801 actgagttac gtaaaaaccc agctaaatac tttattgatc aaccagttgc ggttctttct
     4861 aataatcgcc ccgcaggata tctcttaagt gccagcgcat tcgaagcgtt aatggacatg
     4921 cttgctgaac aagaggagaa aaagcccata aaggcgcgct ttcgtccaag tgctgcaaga
     4981 ttagaggaaa ttacacgccg cgctaaacaa tatcttaatg atatgacgga tgatgatttc
     5041 aatgacttta aggaataagg atgcgggtat tcaaaacaaa acttattcgc ctgcaactta
     5101 cagcagagga acttgatgcg ttaacggcgg attttatttc ctataagcgt gacggtgttt
     5161 tgccagatat atttggtcgc gatgcactct acgacgactc gtttacctgg ccattaatca
     5221 aatttgagcg agttgctcat attcatctgg caaatgtgaa taatccattt ccgccacagt
     5281 tgcgccaatt cagcagaacg aatgacgaag cgcatttggt atattgtcag ggtgcgtttg
     5341 atgagcaagc atggttgctc attgccattc tgaagcctga acctcataaa ctggctcgag
     5401 ataacaacca aatgcataaa attggaaaaa tggcagaagc gtttcgcat
//
LOCUS       DADMGC010000199         5447 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.199, whole genome shotgun sequence.
ACCESSION   DADMGC010000199 DADMGC010000000
VERSION     DADMGC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.199"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            9..746
                     /locus_tag="JNZ73_003919"
     CDS             9..746
                     /locus_tag="JNZ73_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY8734011.1"
                     /translation="MSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNV
                     TYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATLG
                     SGNPRNFTCNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSF
                     YLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGL
                     SATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="JNZ73_003920"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="JNZ73_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HAY8734012.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..3804
                     /gene="safA"
                     /locus_tag="JNZ73_003921"
     CDS             3607..3804
                     /gene="safA"
                     /locus_tag="JNZ73_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HAY8734013.1"
                     /translation="MHATTVKNKIMQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            3878..4639
                     /gene="ydeO"
                     /locus_tag="JNZ73_003922"
     CDS             3878..4639
                     /gene="ydeO"
                     /locus_tag="JNZ73_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HAY8734014.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5041..>5447
                     /locus_tag="JNZ73_003923"
     CDS             5041..>5447
                     /locus_tag="JNZ73_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY8734015.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSR"
BASE COUNT         1569 a         1164 c         1206 g         1508 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg taatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgaa aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ctactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagcacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ctgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg cattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tactcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcatgc aaagagataa ctataaagaa
     3661 atcatgtctg caattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgcaaga ctgaaaataa acagcaaatt ataaatgtga aatgtttaaa
     3841 aggtatcgat aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat
     3901 atttattcat catgctttca acgctaacat tttagataaa gattacgcct tctctgacgg
     3961 cgagatcctg atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt
     4021 taaagaaatc ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca
     4081 gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt
     4141 gcttaatgaa atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga
     4201 acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt
     4261 aactaacggt gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc
     4321 gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa
     4381 gaaaaaacta aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca
     4441 gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg
     4501 ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa
     4561 gagagtttct aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat
     4621 gagtgcttta gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga
     4681 attatatggg gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat
     4741 aaataagtga catccatcac atatttatgc acttgcataa cctgttgcat gattatttat
     4801 aatctcaatt cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag
     4861 ttatttatcg gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact
     4921 gttatttccc tttttttagc taaatctgct atctctttat gtgactaact tcacttacat
     4981 ccacttattt ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt
     5041 atgaagtctg cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct
     5101 ggtatggctg catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca
     5161 aacgttgctt tctcagactt tacgccgact gaatacagta ccaaaggaaa gccaaatatt
     5221 atcgtactaa ccatggatga tcttggttat ggacaacttc cttttgataa gggatctttt
     5281 gacccaaaaa caatggaaaa tcgtgaagtt gtcgatacct acaaaatagg gatagataaa
     5341 gccattgaag ctgcacaaaa atcaacgccg acgctccttt cattaatgga tgaaggcgta
     5401 cgttttacta acggctatgt ggcacacggt gtttccggcc cctcccg
//
LOCUS       DADMGC010000200         5411 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.200, whole genome shotgun sequence.
ACCESSION   DADMGC010000200 DADMGC010000000
VERSION     DADMGC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.200"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..87)
                     /locus_tag="JNZ73_003924"
     CDS             complement(<1..87)
                     /locus_tag="JNZ73_003924"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY8734016.1"
                     /translation="MALESDERMLPPNIRGYAPQITGIAETNA"
     gene            complement(249..3557)
                     /gene="icsA"
                     /locus_tag="JNZ73_003925"
                     /gene_synonym="virG"
     CDS             complement(249..3557)
                     /gene="icsA"
                     /locus_tag="JNZ73_003925"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HAY8734017.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGSS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4086..5288
                     /gene="virA"
                     /locus_tag="JNZ73_003926"
     CDS             4086..5288
                     /gene="virA"
                     /locus_tag="JNZ73_003926"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HAY8734018.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1680 a         1217 c          895 g         1619 t
ORIGIN      
        1 tgcattagtt tctgcaatac cagtaatctg aggcgcataa ccacgaatat ttggcggtag
       61 cattcgttca tcgctttcta atgcaattcc tgtgtaacgc caagcaacag cgcattctgg
      121 cgcatgatct ggtaaacacg tttggcattg atcgcaggca taccatcatg tgcacaaaaa
      181 cccaccgagc ttttacacgg tgggtcccag agaaatgcag gacatcaaca cgccctgcat
      241 ttttattatc agaaggtata tttcacaccc aaaatacctt gggtatctct gtaactgtta
      301 ttacccaatt gctgagatac gtttccccag atgcttaggt tatctgcaag acgccccccc
      361 acaccagttt tgagttcaat cacattacgg ttgcctatct ggtgatttga catgccattc
      421 ataattacac catattgctt tgagctccat ttccaattca cttccaaaaa tggttcgaac
      481 ttacgaagag tctccttatc gatacaactc tgactaatta aggatgtacg caatcccaat
      541 tttgcttgaa tattattgac cccagaggat gtgaccatag tgccattgtg ttcaatatag
      601 tcgtttgctt ttaccccatg cctagttatc tggacttgtg gattgagata caatgcgttg
      661 tcaatattat tatgagcagt ccagcgtatg gttgggtaga tatagccagc ttccaaagct
      721 cctgttattc ctttgctgga atatttctca ccagttaacc catctccttt gactgtatta
      781 ttaaaccaac tgtactgcaa ccaagcatcc atatatagac ctgttcgttc tttttcatcc
      841 tgaaaccaag aactgtacaa accggcagta taaccgctca cagttcctcg tgaactataa
      901 ctagtatact tattatgagt tttgctatcc tgatatccat aagctcccat aatacccaca
      961 tgcaagccat catgatcagt gaagtttgtc ttcaaaatat ctccaccaat ctgataaacc
     1021 atcctgttag ctgtggtttt taattgtcta tcagctaaat tatgctcatt atgtccacca
     1081 acagtacgaa tccacatggt ggtttcgctg agctgacctg tagtaggatc aacaatagtg
     1141 ctaccgtgtc ggtcatagag agaaggacta aacatcgtgt tcattgctat caggttagca
     1201 atataactgc cagcttcagg gcgccatgtg tgaatactac cttcacccaa attaagtgaa
     1261 gatacaagct gtgaactagc tcggcgacta ctcatttgag tagactcttg attgctcatc
     1321 tgttttgatt cttgattatc catctgacta gttagatacc acttattggt attcagtcca
     1381 gatacagtgc cctgtttcag gcgatagtca taactaccag caacaatacg gcctttctga
     1441 ataaaagcat tcttatcaga agaatctgtc gaaataattt gaacaccttc aagagtttta
     1501 tctccagagc ctccctcatt tgtcactgca acataggtaa taccagacgc atggcctgca
     1561 atttccagcc ggtcagtttt actgttgtca ttgccgagaa ccgcgcccaa agataatatt
     1621 ccgccctttc catgccaatt acctttctga acataggtct gaccgacatt tgacgaacta
     1681 ttattgagaa taacatgacc gtttttctcc agagtcatgt tacctgtgac aatgacgggg
     1741 tcaggcaaaa aaggggcatt aatattattg attagcacag tcccactatt taataaagtg
     1801 ttaacagttt ggttcatgcc tgaaaaattc aaggtagcac ctttattaac aataacacca
     1861 gcggtacgtg tcatagcttc aactgtcccc atttttagaa tgcctgcatt gatgttggtg
     1921 aaaccagagt aggtattttt ttccgccaaa atgagagttc cctcccctga ttttgttagg
     1981 gattttccgt cccagctatt tatattatga tggctgagat tatctgccag aatagtacta
     2041 acagtaagtt cagcgttttc tttcagattg aactctccat gactgtcgcc atctgtatca
     2101 ttccagctca aaccgtagat caatttattc tggtcactga tatagctttt tgtatagtca
     2161 atagcaccag tagtgagtaa cggattgatg atagatatat tagaaacact ggaacctgtt
     2221 atccctttat ctgcacttag cagtttatag tccttctgcg cccagttatt tataatagct
     2281 tccactttta cattgttgcc aaatgacata tttccagaag cttccagact actgattcca
     2341 gctaaattta aagaactgtt ctcaccaaaa gaaacgtcac ctgaaacagt gaatttgtta
     2401 cctgatagtg aaacagttgt acaatcacca gcacataaat caccttcaat agttggtgtg
     2461 gtcccagtgt aatcattgtt gataattttc agaatggatg aattgttaac ctgtacatca
     2521 ccagtaatga cagaaccttc atggagtatt aatgaattgt ttccaccagt gatgtggata
     2581 gcattacctg ctacattttg tgttccatca tcttctttac ctgaaattat atatccgcta
     2641 ttagcaatgg tcatattgct accatttata gcatcccccc aatgtccacc attaccgcct
     2701 aaaatagctc cattgttgat tacagacagg ttatctcctg tgatagcatt accaccgtag
     2761 ccatcataac cataaccgta agcagcacct ccgttgcctc cagcgaacgt gcccttattg
     2821 attacagaga ggctgcttcc tgtgatggca tcacctccat taccgccatc accctcacca
     2881 taattgttac ctccattacc accggaaata gtccctttgt tgattatggg tagattgact
     2941 cctgtgacgg catcacctcc attcccccca tcactatcac tataactatc gccaccatgt
     3001 ccacctgaaa taatttctcc atttattata aacagattgt ctcctgtaac agcctcacca
     3061 ccatcaccgt tatggtcagc accgctaccg ccgctaccac caagaatcat gccttgattg
     3121 attatagaca agtcagatcc ggtaatagag tcaccaccat taccgccaca actattacca
     3181 tcattattat caccagcacc accatgacct gcgctaatca taatatcaga gttaatccgt
     3241 agcttagttc tagatgcatg agaggggttg gaagaagtta tataccacgc atccattcca
     3301 tcttcaccag ctcccaaggg agaaagtcca tcaacaggtg ttaataattt ttcataattg
     3361 tcctctgaaa aatgaagttc ttgagtaccc gaaagaggag tagcaaaagc tattggcccc
     3421 ccgagcaaca gggatgcacc aacaacaaaa ggagaaaaag acaatttgca tgttttttcc
     3481 tttaccgtag gtaattctcc ggcccccccc tgtgaacatt gggtcatatt acaaaaaaat
     3541 ttgtgaattt gattcatgca ctatattatc agtaagtggt tgataaaccc ctgaaaagat
     3601 tccgttttat ccggaataaa gggacgatat atgcaaaaac atattaaaca aagcctcaac
     3661 caaaacatga gagctgtgtt atatatcaat gcaacgggag aaatttacct gtgtcttgct
     3721 ttgaaaaaaa gaggggaaaa gctatatgtt cgataataaa ttaataattt tactatgata
     3781 gtctgcttca gaccgcaact tttcagtcct ttctgtacgc ttgcccaaag tttaaccact
     3841 ttgtttttct atacacccaa tggttctatc tgccccttga tgttaatccc caaagccaat
     3901 aacatgaatg tattcatatc acaactttcc taccagactc tccaatgcaa taactgcata
     3961 attgttttta gtggaaatgt atcgttttct taaagagaag aataacattc cattttatta
     4021 tgttcccaat ttaattatat taatatatat tggcattaat aggaaaatac atcaggagaa
     4081 atcaaatgca gacatcaaac ataactaacc atgaaagaaa tgactcatca tggatgtcaa
     4141 ctgtcaaatc aactacagaa gtaagttgga ataaattgag tttttgtgac gttttactga
     4201 aaataataac tttcggcata tattctccac acgaaactct agccgaaaaa tatagcgaaa
     4261 aaaaactaat ggatagtttc tcaccatcac tatcacaaga taaaatggac ggagagtttg
     4321 cccacgcaaa catagatggc atttcaatac ggctatgcct taacaaaggc atatgttcgg
     4381 ttttttatct tgatggagat aaaatacaat ccactcaact ttcatcaaaa gaatataata
     4441 acttgttaag ttcactcccc cccaaacaat tcaacttagg aaaagtacat actattacag
     4501 ctccggtatc agggaatttt aaaactcaca agcctgcacc agaagttatt gaaacagcca
     4561 ttaattgctg tacatcgata ataccaaacg atgattactt ccacgttaaa gacactgatt
     4621 ttaatagtgt ttggcatgat atttacagag atattcgagc atctgacagc aatagtacaa
     4681 aaatatattt caacaatatt gaaataccac taaaattaat tgcagatcta attaatgagt
     4741 taggaattaa tgaattcatt gatagcaaaa aagagcttca aatgctaagt tacaaccaag
     4801 taaataaaat aatcaattca aacttccccc aacaagacct atgttttcaa acagaaaaac
     4861 ttttgtttac ttcactcttt caagaccctg catttatatc tgcactaaca tctgcattct
     4921 ggcaatctct tcacatcacg tcttcctctg tggaacacat atatgcccaa attatgtccg
     4981 aaaacataga aaacaggctt aatttcatgc ctgaacaacg agttattaac aattgtggac
     5041 atattataaa aatcaacgca gttgtcccta aaaacgacac tgcaatctct gcctctgggg
     5101 ggagggctta cgaagttagc tcatcaatat taccatcgca cataacatgt aatggtgttg
     5161 gtattaataa aatagaaaca agctatctcg ttcatgctgg aacacttcca agcagtgaag
     5221 ggcttcgcaa tgctatccct ccagaaagtc gtcaagtatc atttgccatc atatctcctg
     5281 atgtttaagc caatattata ttggctgcca gactgtaaaa aaacgttctt cagaacagct
     5341 ctataaacta ttaaaccaaa gctaaaagga tggggaccaa tagaatctca gcccatcctt
     5401 tttatatcat t
//
LOCUS       DADMGC010000201         5337 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.201, whole genome shotgun sequence.
ACCESSION   DADMGC010000201 DADMGC010000000
VERSION     DADMGC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.201"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="JNZ73_003927"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="JNZ73_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HAY8734019.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="JNZ73_003928"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="JNZ73_003928"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HAY8734020.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINSEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="JNZ73_003929"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="JNZ73_003929"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HAY8734021.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="JNZ73_003930"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="JNZ73_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAY8734022.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEINEDRIVAV
                     Q"
BASE COUNT         1309 a         1289 c         1456 g         1283 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaactc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgactttc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaaattaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggg
//
LOCUS       DADMGC010000202         5318 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.202, whole genome shotgun sequence.
ACCESSION   DADMGC010000202 DADMGC010000000
VERSION     DADMGC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.202"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            1..607
                     /locus_tag="JNZ73_003931"
                     /pseudo
     CDS             1..607
                     /locus_tag="JNZ73_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            671..1597
                     /gene="glsB"
                     /locus_tag="JNZ73_003932"
     CDS             671..1597
                     /gene="glsB"
                     /locus_tag="JNZ73_003932"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HAY8734023.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            1564..1956
                     /locus_tag="JNZ73_003933"
     CDS             1564..1956
                     /locus_tag="JNZ73_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HAY8734024.1"
                     /translation="MDETVRAFGLLRQSLDPLFARLSRSKFRSRFRLGMKERQYCLEK
                     GAPVIEQHAADFVAKRLAPALPANDGKQPPMRGHPVFIAQHATATCCRGCLAKWHNIP
                     QGVSLSEEQQRYIVAVIYHWLVVQMNQP"
     gene            2095..3513
                     /locus_tag="JNZ73_003934"
     CDS             2095..3513
                     /locus_tag="JNZ73_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY8734025.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            3740..5191
                     /locus_tag="JNZ73_003935"
     CDS             3740..5191
                     /locus_tag="JNZ73_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAY8734026.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT         1228 a         1238 c         1433 g         1419 t
ORIGIN      
        1 cccatcattc ttgcaggtaa cggctactta cttaaacatg cgccgaatgt gatgggctgt
       61 gctcagctca ttgcccaggt gtttaaagat gcgggaatcc cgcaaggcgt atatggctgg
      121 ctgaatgccg acaacgacgg tgtcagtcag atgattaaag actcgcgcat tgctgctgtc
      181 acggtgaccg gaagtgttcg tgctggagcg gctattggcg cacaggctgg agcggcactg
      241 aaaaaatgcg tactactatc cgccaacggt tctggcgaat gttaccccag aaatgaccgc
      301 gtttcgggaa gaaatgtttg gccccgttgc tgcaatcacc attgcgaaag atgcagaaca
      361 tgcgctggaa ctggctaatg atagtgagtt cggcctttca gcgaccattt ttaccaccga
      421 cgaaacacag gccagacaga tggcggcacg tctggaatgc ggtggggtgt ttatcaatgg
      481 ttattgtgcc agcgacgcgt gagtggcctt tggtggcgtg aaaaagagtg gctttggtcg
      541 tgagctttcc catttcggct tacacgaatt ctgtaatatc cagacagtgt ggaaagaccg
      601 gatctgacgc gacatattca gctctgatat actcgcaggt cttttcagac ctgcggtcca
      661 ggagtagaaa gtggcagtcg ccatggataa tgcaatttta gaaaacatct tgcggcaagt
      721 gcggccgctc attggtcagg gtaaagtcgc ggattatatt ccggcgctgg ctacagtaga
      781 cggttcccga ttggggattg ctatctgtac cgttgacgga cagctttttc aggccggaga
      841 cgcgcaagaa cgtttttcca ttcagtctat ttccaaagtg ctgagtctcg ttgtcgccat
      901 gcgtcattac tccgaagagg aaatctggca acgcgtcggc aaagatccgt ctggatcacc
      961 gttcaattct ttagtgcaac tggaaatgga acagggtatt ccgcgtaatc cgttcattaa
     1021 tgccggtgcg ctggtggtct gcgatatgtt gcaagggcga ctaagcgcac cacggcaacg
     1081 tatgctggaa gtcgtgcgcg gcttaagcgg tgtgtctgat atttcatacg atacggtggt
     1141 agcgcgttcc gaatttgaac attccgcgcg aaatgcggct atcgcttggc tgatgaagtc
     1201 gtttggcaat ttccatcatg acgtgacaac cgttctgcaa aactactttc attactgcgc
     1261 tctgaaaatg agctgtgtag agctggcccg gacgtttgtc tttctggcta atcaggggaa
     1321 agctattcat attgatgaac ctgtggtgac gccaatgcag gcgcggcaaa ttaacgcgct
     1381 gatggcgacc agtggtatgt accagaacgc gggggagttt gcctggcggg tgggactacc
     1441 ggcgaaatct ggcgttggtg gcggtattgt ggcgattgtt ccgcatgaaa tggccatcgc
     1501 agtctggagt ccggaactgg atgatgcagg taactcgctt gcgggtatcg ccgtccttga
     1561 acaattgacg aaacagttag ggcgttcggt ttattaaggc agtctctcga tccactcttc
     1621 gcgcgtttat cccgttcaaa atttcgctct cgctttcgtc tgggcatgaa agagcgtcag
     1681 tattgcctgg agaaaggcgc gccagtcatc gaacaacatg cggcggattt tgtcgctaaa
     1741 cgtcttgctc cggcattacc ggctaatgac ggtaagcaac cccccatgcg cgggcatccg
     1801 gtgtttatcg ctcagcacgc caccgcgacc tgctgtcgcg gttgtctggc taagtggcat
     1861 aacattccgc agggtgtttc gttaagcgaa gaacaacaac gttatatcgt ggcggtaatc
     1921 tatcactggc tggttgtaca gatgaatcag ccatgagaat tgcggaaaac gataaaatag
     1981 cattcccagg gtacctttat ttgccgccgc tgttagcgaa catgctatcc tttatgagaa
     2041 tttacttatc acgttgagat aattgccacc cgcaattgta tggacagata attaatgcat
     2101 gtacacccca tttcaacttt ccgtttgttc caggaaggtc atctgctacg taatagcatc
     2161 gctatttttg cgctaaccac gctgttctat tttattggtg cagagttacg gctggttcac
     2221 gaactttctc ttttctggcc gctgaatggc gtaatggcgg gggtgtttgc ccgctatgtc
     2281 tggcttaatc gactgcatta ctatgcgatc agttatgtgg cgatgctggt ttatgatgcc
     2341 ataaccaccg aatggggact ggtttcactg gttatcaatt tctccaatat gatgtttatt
     2401 gttaccgtcg ccttactggt cgtgcgggat aagcgtctgg ggaaaaataa gtatgagcca
     2461 gtaagtgcct tacggctatt taattactgt ctgattgccg cattattatg cgctattgtc
     2521 ggggcgattg gttcggtcag tattgatagt ctggatttct ggcctttgct tgcggactgg
     2581 ttcagtgagc aattctcaac gggcgtgttg atcgtgcctt gtatgctgac gttggctatt
     2641 cctggagtgc tgccgcgctt taaagcagaa cagatgatgc ccgccatcgc gcttattgtg
     2701 tcggttattg cctcggtagt cattggcgga gcggggagtc tggcgttccc gctgcctgca
     2761 ttaatctggt gtgcagtgcg ctatacgccg caggtaacat gtctgttgac ctttgtcacc
     2821 ggtgcggtgg aaatcgtact ggtggcaaat tcggtgattg atatctcggt cggttcgccg
     2881 ttctccattc cggaaatgtt ctccgcacgt ctcggtattg ccacgatggc gatatgccca
     2941 attatggttt cttttagcgt ggcagcgatc aatttgctaa tgaagcaagt tgcgctgcga
     3001 gccgactttg attttctgac tcaggtttac tcacggtccg gtctttatga ggcgctgaaa
     3061 agtccatcgc tgaaacagac acaacatctg acggtcatgc tgcttgatat cgactatttc
     3121 aaaagcatta acgataacta tggacatgaa tgtggcgata aagtgttaag cgtgtttgcc
     3181 cggcatattc agaagattgt cggtgataag gggctggtgg cgcgcatggg cggcgaagaa
     3241 tttgctgttg cagtgccgtc ggtgaatcct gtcgatggtc tgctaatggc ggaaaaaatc
     3301 cgtaaaggcg ttgaactgca accattcacc tggcaacaaa aaacgcttta tctcacggta
     3361 agtattggcg tcggtagtgg tcgcgcatcg taccgaacgc tgaccgatga ttttaataaa
     3421 ttgatggtcg aagccgatac gtgtctgtat cgctcgaaga aagatgggcg caaccgtacc
     3481 agcactatgc gttacggtga ggaagttgtc tgacatcttt attcattccg cgcgatttga
     3541 atgcaatgtg ttctaccact ttatgatcca ggaaaaggcg tatttcctcg attaaaccat
     3601 gatccgcgcc acacttttct cataaaactg ctcattaatc atcaggagtt gatcaaaaca
     3661 ggggttcggc gtattaaagt ttgctaacct gtcgcaccaa ttcaaacagg ttgtatgact
     3721 aatcagaagg gaacccattg tgaaaacact aaatcgtcgc gattttcccg gtgcacagta
     3781 tccagaacgt atcattcagt ttggtgaagg taacttcctg cgcgcctttg ttgactggca
     3841 aatcgatctc ctgaatgagc acaccgatct gaattctggc gtggtcgttg ttcgtccgat
     3901 tgaaacttca ttcccgccgt cactgagcac gcaggatggt ctgtacacca ccattatccg
     3961 tggcctgaat gagaaggggg aagcggtcag cgacgcgcgt ctgattcgct cggttaatcg
     4021 tgaaatcagc gtctacagtg aatacgatga attcctgaaa ctggcgcaca acccggaaat
     4081 gcgctttgtt ttctctaaca ccaccgaagc gggtatcagc taccatgcgg gcgataaatt
     4141 cgatgacgcg ccagcggtaa gctatccggc aaaactgacc cgtctgctgt tcgaacgctt
     4201 tagccatttc aacggtgcgc tggataaagg ttggatcatc attccgtgtg agttgattga
     4261 ctacaatggc gacgcgttgc gtgaactggt gctgcgctat gcgcaagagt gggcactgcc
     4321 agaagcattt attcaatggc tggatcaggc taacagcttc tgttctacgc tggtggaccg
     4381 tatcgttacc ggttatccgc gcgatgaagt ggctaaactg gaagaagaac tgggttatca
     4441 cgatggtttc ctcgacaccg ctgaacattt ttacctgttt gtgattcagg ggccgaaatc
     4501 cttagcgact gagctgcgcc tggacaaata tccgctcaac gtgctgattg ttgacgatat
     4561 taaaccgtat aaagaacgca aagtggcaat cctcaacggt gcgcacaccg cgctggtgcc
     4621 ggtggcgttc caggcagggc tggataccgt aggtgaagcg atgaacgatg ctgaaatttg
     4681 cgcattcgtt gaaaaagcca tctacgaaga aattattccg gtactggatt tgcctcgtga
     4741 tgaactggaa tctttcgcca gtgcagtcac cgggcgtttc cgtaacccgt acattaagca
     4801 tcagctgctg tctatcgcgc tcaacggtat gaccaagttc cgcacccgca tcctgccgca
     4861 gctgctggca gggcagaagg caaacggcac acttccggcg cgccttactt tcgcattagc
     4921 ggcattgatt gcgttctatc gtggtgagcg taacggagaa acatatccgg tacaggacga
     4981 tgcacactgg ctggaacgtt atcagcaact ctggagccag catcgtgacc gtgttatcgg
     5041 tacccaggaa ctggtagcga ttgtgttggc agaaaaagac cactgggagc aggacctgac
     5101 gcaagtgcct ggtctggtcg agcaggttgc taatgacctg gatgcgattc ttgaaaaagg
     5161 gatgcgcgaa gctgtacgtc cgttgtgcta ataacaccgt tacttctgaa cccggtttcg
     5221 accgggtttc ttatttcact atattagtta caacatccta tatgccttta tcattaacag
     5281 gtgtcacatg cagcgcctgt taatgttgta agcttgac
//
LOCUS       DADMGC010000203         5214 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.203, whole genome shotgun sequence.
ACCESSION   DADMGC010000203 DADMGC010000000
VERSION     DADMGC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.203"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            83..190
                     /locus_tag="JNZ73_003936"
                     /pseudo
     CDS             83..190
                     /locus_tag="JNZ73_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            187..477
                     /gene="tnpB"
                     /locus_tag="JNZ73_003937"
     CDS             187..477
                     /gene="tnpB"
                     /locus_tag="JNZ73_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY8734027.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKISITRSQLAML"
     gene            complement(729..1472)
                     /locus_tag="JNZ73_003938"
     CDS             complement(729..1472)
                     /locus_tag="JNZ73_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005067636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAY8734028.1"
                     /translation="MVKILAVAQSHESIVSMDEINSILLSSSWFRALPDYLIEQMLSR
                     TSLYQYNDGEIIHYRGAEPLGLYAVITGSVKVSSISADGKECIFRYLSPGNWFGEIGM
                     IDKCPRTHDARATGKTTLLTIKPHDVEFLLKNYPILHRFLALLLCKVVRNAFTMLHDC
                     TLLSVSARLAKRLVSLSEGYGEPHERGTLINLYLTQDDLATIINTSRQTINKRLVEWE
                     KLGWIDARYGKIVIININALRQLYADEDE"
     gene            complement(1522..2889)
                     /locus_tag="JNZ73_003939"
     CDS             complement(1522..2889)
                     /locus_tag="JNZ73_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8734029.1"
                     /translation="MPSHTDQYLSLAIQKSIRRIIPMVILMFILAYLDRSNIGFAKQE
                     FQLDTSLSDTAYAFGAGIFFIGYALFEVPSNILLYRIGARVWLARIMVTWGLVSAAMM
                     FAHTETVFIFLRFLLGISEAGFFPGVILYLTFWFPQHIRARVTGYFLFGAPLAFIIGG
                     PLSGSLLSLEGSVLSFGLHGWQLMFLVEGLLASAVGIWVFFYLDDHPEKAKWLTPQEK
                     AALSQTLNKEEQVKLAHGPKGAWQALLDVRVLYLCLIWFTVQVCGYGIYFFLPTQIAT
                     LIGTKVGLLVGFVTAIPPLCAAIAVYYVPRISEKLQERRYVASITFMSGAIGIAVSGL
                     ADSIPLVAIVALCVAAAGHLAMQPLYWTFPSSYLGGAAAASGIALINSIGNLGGFVAP
                     NLRVWAEHTFNSAQAGLYLLAMVTATGSLMILTLRHFGIEKTPRTSSTSPNLPASGDN
                     LHHSD"
     gene            complement(2961..4073)
                     /locus_tag="JNZ73_003940"
     CDS             complement(2961..4073)
                     /locus_tag="JNZ73_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HAY8734030.1"
                     /translation="MKITKLTTFIMHVPVTKNIIGDSTHSITHWGMPGVMIETDTGLV
                     GYGYTGTHADITTDRLITTIIKDAFGPMLIGEDASEVRYLHRKMSRSSTNIWVGRGGL
                     MQMALSAIDIALWDLKAKMVEQPLWKVFGGSQHSRVLAYNTDCGWLVRNTAELVDDCK
                     KMIFEEGFSAIKMKIGKPDPREDLKRIEAVRKAIGDDVNLMVDANAKWDISTAKQYGA
                     KLADFDITWFEEPMWHDDIASHRTLSGHISTPIALGELLYQFDSFKEFVLAGAVDYLQ
                     PDATRCGGLTAVWEIADLGQAFNLPVTPHHGDMMQAQLHLVMAHPSCSLLEFIPWTLG
                     CFVDPVEVKEGYYSLPTAPGAGTTLRPEALEHFSVR"
     gene            4239..4720
                     /locus_tag="JNZ73_003941"
                     /pseudo
     CDS             4239..4720
                     /locus_tag="JNZ73_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
     gene            4781..5214
                     /locus_tag="JNZ73_003942"
                     /pseudo
     CDS             4781..5214
                     /locus_tag="JNZ73_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1456 a         1252 c         1265 g         1241 t
ORIGIN      
        1 agaaataacg ccggatgagc agaaaaaaac tgtggcggcc attgcgccgt ctttatccac
       61 ttccactcag accagagtca gtgccagttc ctgcaaggtg gagttccgtc acggtaacat
      121 gacgctggaa aatccatcgc cagagctgct cacggtgttg atccgcgaac tgagcgggag
      181 gggaagatga tttcactccc atcaggtacc cgtatctggc tcgttgccgg cgttaccgat
      241 atgcgtaaat ccttcaacgg actgggagaa caggtacaac atgtgctgaa tgataatccc
      301 ttctccggcc acctgtttat cttccgtggc cgacggggtg acaccgttaa aattctttgg
      361 gctgatgctg atggtctgtg cctgttcacc aaacgccttg aggaaggtca gtttatctgg
      421 cctgcggtgc gtgacggtaa gatatccatt acccgctcgc aactggcaat gctgtaaacg
      481 ttaacggagc gccgtattga cgcttacgaa ctgtgtgatc cgacatgcca ttaggaaacg
      541 taaggcgttc ccgatgggcg agtcagtaaa agtgaaatgg ctggcatcct ggtcgcgtcg
      601 cagaaatgga caatgccgca gagggactgg cgaatagcaa tttattatcg aatttgatga
      661 ccgcctggac agtcactttt aagaaaaagc atgtatacag aatggtaaac aggctcatcc
      721 acagccaatt actcatcttc atcagcatat aactgcctga gtgcatttat attgatgatc
      781 acgattttgc catatctggc gtctatccat cccaattttt cccattcaac cagccgtttg
      841 ttgatcgttt gtcggctcgt attgattatc gttgccagat cgtcctgcgt aagataaaga
      901 ttgattaatg tccctcgctc gtgtggttcg ccgtaccctt ccgacagact cactaaacgc
      961 ttcgccaagc gtgcagaaac agataatagc gtacagtcat gtaacatcgt aaaggcattt
     1021 cgaacgactt tgcaaagcag caacgccaga aaacgatgga gaattggata atttttcaga
     1081 agaaattcaa catcatgagg cttgatcgtt aacagtgtag ttttacccgt cgctctggca
     1141 tcgtgtgtgc gcggacattt atcaatcata cctatttcac caaaccagtt ccccggcgaa
     1201 agatagcgga agatgcactc tttaccgtca gcagaaattg agctcacttt aacggagcca
     1261 gtaatgactg catataatcc gagcggctca gcaccacgat agtgaataat ctctccatcg
     1321 ttgtattgat acaggctggt cctggacaac atctgttcaa taagataatc aggtagcgcc
     1381 cgaaaccaac tactactaag aaggatacta ttgatctcgt ccatagaaac tatcgattca
     1441 tgtgattgtg ctacggccaa tatcttaacc attgttgtta tcctgatctt aatcatgacc
     1501 gtagcaatgt agcatttcct gtcaatcaga gtgatgcaga ttatccccgc tggcagggag
     1561 attcggagat gtggatgatg tacggggcgt tttttcaatc ccgaagtgac gtaatgtcag
     1621 aatcatcagg ctaccagtag ctgttaccat tgccagtaag taaagtcctg cctgtgcgga
     1681 attaaaagta tgttccgccc agacgcgaag attcggcgct acaaaccccc ccagattgcc
     1741 tatcgagtta attaacgcaa tgccggaagc agctgctgca ccgccgagat aagacgatgg
     1801 aaatgtccag tacagtggct gcatcgccag atgtcctgca gcggcaacgc acagcgcgac
     1861 aatagcgacc agtggaatac tatcggcaag gccagatacg gcaatgccta ttgcaccgga
     1921 cataaatgtg attgatgcaa cgtaacgacg ttcctgcagt ttttccgata tacgtggtac
     1981 gtaatacact gcgatggcag cgcacaaagg tggaatagcg gtgacgaagc caactaataa
     2041 accaactttt gtccctatta aggtggcgat ttgtgttggc agaaagaagt aaattccata
     2101 accacatacc tgcaccgtaa accagatcag gcataaatag aggacgcgta catccagcag
     2161 tgcttgccat gcgccttttg gaccatgtgc aagtttcacc tgctcttcct tatttagagt
     2221 ctggctaagc gccgctttct cttgtggagt taaccatttg gctttctctg ggtgatcgtc
     2281 aagatagaag aacacccaga tcccaacggc agaggccagt aacccctcaa cgaggaacat
     2341 gagttgccag ccatggagac caaacgacag taccgatcct tcaagcgaga gtagcgagcc
     2401 ggaaagcgga ccgccgatga taaaagctag tggggcacca aacaggaaat agccggtgac
     2461 gcgtgcgcgg atgtgctggg ggaaccagaa tgtaagataa agaataactc caggaaagaa
     2521 acctgcttca ctgatgccaa gcaagaagcg caggaaaata aatacggtct cagtgtgggc
     2581 gaacatcatt gcagcggaaa ccagccccca ggtcaccatg atacgggcca gccagacacg
     2641 ggcgccgatg cgataaagca gaatattact gggcacctcg aacagggcat aaccaataaa
     2701 gaagatcccg gcgccgaaag cataggcggt gtcactcaga ctggtatcca gttgaaattc
     2761 ttgtttagca aagccgatat tggagcgatc cagataggcc agaataaaca ttagtatgac
     2821 catcggaatg atgcgacgta tggatttttg aattgcaagt gataaatact gatcggtatg
     2881 gctgggcata aatcacctct tagaattttg tcattattat tttgccccgg tcattgcata
     2941 accgggcgat acaagtgttc tcatctaacg ctaaagtgct caagagcttc cgggcgtaag
     3001 gtagtgccgg cacccggcgc ggtgggaaga gagtaatatc cctctttgac ctcaaccgga
     3061 tcgacaaagc agcccagcgt ccagggaata aactccaaca gagagcaaga gggatgtgcc
     3121 attaccagat gtaattgtgc ctgcatcata tcaccatgat gtggtgtgac cggcagattg
     3181 aacgcctgac cgagatcggc tatttcccat acggcggtca gtccaccgca gcgagtggca
     3241 tcgggctgga gataatccac cgcaccggcc agtacaaact ctttaaagga atcaaactga
     3301 tagagtaact cacccaacgc aatcggcgta ctgatatgcc cggacagcgt gcggtgactt
     3361 gcgatatcat catgccacat cggctcttca aaccaggtaa tatcgaaatc agccagcttg
     3421 gcaccgtact gcttggcagt gctgatgtcc cattttgcat tcgcgtcgac catcagattg
     3481 acgtcatcgc ctatcgcttt tctgacggct tcgattcgtt tgagatcttc tcgcggatcg
     3541 ggcttgccta tcttcatctt gatggcggaa aatccctcct caaaaatcat cttcttgcag
     3601 tcatccacta actccgccgt gttacgaacc agccagccgc agtcggtgtt gtaggcaaga
     3661 acacgggaat gttgagaacc gccaaagact ttccacaaag gttgctccac cattttggct
     3721 ttcaggtccc acagggcgat atcgattgct gaaagcgcca tttgcattaa cccaccgcgg
     3781 cccacccaga tattggtcga gctgcgtgac atcttacggt ggagatagcg aacttcactg
     3841 gcgtcttctc caatcaacat cgggccgaag gcatctttaa taatggtggt gataagacgg
     3901 tcggtagtaa tgtctgcatg ggttccggta taaccgtaac ctacaaggcc ggtatctgtc
     3961 tcgatcatta cgcccggcat tccccaatga gtaatgctgt gtgtggagtc accgataata
     4021 tttttagtta ccggtacatg cataataaag gtagtgagtt tagtgatttt cattgcagtt
     4081 tcctgtgtct gtatatcttg attgatgggc gcaatgtagc aaccggcctt cagcagggct
     4141 gtcaaatggt tgacacatca tcagaagaga gaaggaataa ttgtattttt ttgaagtaga
     4201 tctcgaattt ggaccacacc cagtcgacaa gttttcatat agggaactgg gccatgaccg
     4261 gagcttttca ggatgagatc tctgtacgca tcatgaagac catgcggcta aagtgatcct
     4321 ggagtacagc aaactggaaa aaatcgaaaa gtattacgaa aaatacgcaa acgcaggcac
     4381 caatgagtgg cacatcgatg aaagtaaaaa agctaccgca accatgaatg tagttctggg
     4441 tgatgaagat aggttaaaat ccctcgcgga agattttgcc agacattatg aaaaacgcgt
     4501 agccgaaggt ttcaccgtaa aaggcaagac catgtttgtg ccagccgtga aattgcctag
     4561 ggttctaccg tcaccttgaa gctattcgca ctgcctggtt ttaagtgaaa caagtacccg
     4621 atggcatcgt cctgactgaa cgggagcaga aaaattaccg ccttctgaaa tggtgaaaat
     4681 ggtcatgacg cgtggtaaag atgatgacga ggcgctttat agatttgtgt aattgcctga
     4741 ttttgatatg ttcaattcaa catcaaatga aggttaattt atggacgaaa aatagttaca
     4801 gactctggct aacgaactgg ccaaaaacct caaaacccct gaagacctca gccagtttga
     4861 ccgtctactg aaaaagatca gcgttgaagc cgctctcaac gcagagatga cacaccatcc
     4921 tgggtatgga aaaatcagtc cagaccagga gctaactccc gcaacggtta ttccacaaag
     4981 accgttatca caggcgacgg tccactggaa ctgcgtactc cgcgcgatcg tgacggtagc
     5041 ttcgaaccac aacaggtgaa gaaaaatcag ccccgtatta ccaggatgga taaccagatc
     5101 ctctcgttgt atgccaaagg gataaccacc cgtgagatag ccgccgcgtt caaagacctg
     5161 tatgacgctg atgtttcacc ggcactgata tcaaaggtca ctgatgccgt gatg
//
LOCUS       DADMGC010000204         5145 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.204, whole genome shotgun sequence.
ACCESSION   DADMGC010000204 DADMGC010000000
VERSION     DADMGC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.204"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            1..254
                     /locus_tag="JNZ73_003943"
                     /pseudo
     CDS             1..254
                     /locus_tag="JNZ73_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            747..1493
                     /locus_tag="JNZ73_003944"
     CDS             747..1493
                     /locus_tag="JNZ73_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY8734031.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1508..1930
                     /locus_tag="JNZ73_003945"
     CDS             1508..1930
                     /locus_tag="JNZ73_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAY8734032.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGKFQEAVL"
     gene            1988..2344
                     /locus_tag="JNZ73_003946"
     CDS             1988..2344
                     /locus_tag="JNZ73_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734033.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2437..2655
                     /locus_tag="JNZ73_003947"
     CDS             2437..2655
                     /locus_tag="JNZ73_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 domain-containing protein"
                     /protein_id="HAY8734034.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            2657..3019
                     /locus_tag="JNZ73_003948"
                     /pseudo
     CDS             2657..3019
                     /locus_tag="JNZ73_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3019..3684
                     /locus_tag="JNZ73_003949"
     CDS             3019..3684
                     /locus_tag="JNZ73_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734035.1"
                     /translation="MATITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSGEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            3684..4049
                     /locus_tag="JNZ73_003950"
     CDS             3684..4049
                     /locus_tag="JNZ73_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY8734036.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            4447..4659
                     /locus_tag="JNZ73_003951"
     CDS             4447..4659
                     /locus_tag="JNZ73_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY8734037.1"
                     /translation="MSHKCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            4896..>5145
                     /locus_tag="JNZ73_003952"
     CDS             4896..>5145
                     /locus_tag="JNZ73_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734038.1"
                     /translation="MDITVCTYKPGGIVEKIKKLFSSKYAVIRRDDLSVIVEMDYFPE
                     TPKSIMYRNGRKAIFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT         1407 a         1086 c         1422 g         1230 t
ORIGIN      
        1 atgccaacac aaaggcaact aataaagata cagataaaga tctaaaaaca gatctaaccc
       61 atcccaaacc cttcccttcc ggaagggagt ttcgggattt tgtggctgga gtgcttgagg
      121 ggagattatc tggcggtact gcagcggaat tttgtaattc tgcggtggtt gcgttgcagg
      181 ctgctggcct ggatgtctgt cgtgagtatc cggtgccaga gcgtggtgac ggttgcggag
      241 ggcggattga tatcgtcgtg actgacagga acggtgtccg gtgtgggatc gagcttgacc
      301 gaaattctcc gcgacagaaa tcactgctca aaatcggtgc tgttgaaacc gggatatgtg
      361 tcttgcggcg cagtgatatc gcaaggcaca ccgagcaggg aattctggtt atcggtgggg
      421 ctgttcgcca gaaaaaattt gacccgttgt cagttgatct gcccgactgg ttgccagata
      481 cactctggca tgagtgggtc cagttcaggc aggcattgcg aaaaccgatt cgaacggagc
      541 tgggcgctaa cggggcgata cgggaactgg aaaaattccg tcagcagggt tttacacctg
      601 agcaggtgat tcgacacagc atcgccaatg aataccaggg tctgttcgcg ccgaaaggtg
      661 ttcggcctga gacgttgctc cgacaggtta acaccgtctc gttgccggac agtgcgatcc
      721 cgccaggctt cagggggtaa cagaccatga aaaatattgc gacaggaggc gttctggagc
      781 gtatccgcag actgacccca ccacatgtaa ccgccccatt cagaacggtt gcggagtggc
      841 gcgagtggca acttgctgaa ggccagaaac gttgcgagga gatcaaccgc ctgaatcgtc
      901 agttgcgggt ggaaaaaatt ctgaaccgct ccggcatcca gccgttgcac cggaagtgtt
      961 cgtttgcgaa ttaccgggcg cagaacgacg gtcagcgaca tgcactgagt caggcgaaat
     1021 ccatagctga cgaactgatg acaggctgta cgaattttgt gttcagcggt aagcccggca
     1081 ccggaaaaaa tcatcttgca gccgcgattg gcaaccggct gatggcgaag gggcgtagcg
     1141 tgattatcgt caccgtgtcc gatgtcatga gcgtgttgca tgagagctac gacaacggca
     1201 aatccggtga aaaattttta caggagcttt gtggtgttga cctgctggtc ctggatgaaa
     1261 ttggcatgca gcgggatacg aaaaacgagc aggtggtact gaaccagatt gttgatcgcc
     1321 ggacggcatc gttacgcggt gtggggatgc tgacaaatat taaccatgca gcgatgaata
     1381 cacttctcgg cgagcgggtg atggatcgca tggtcatgaa cggcgggcgc tgggtgaatt
     1441 ttaactggga gagctggcgt ccgaatgtta gccattcgag ggttgttaag tagtttcagg
     1501 aggatttatg gcgaaacctt ttactcccga acagcgggaa gaactgaaga cgcgaattgt
     1561 ggaactcgtg catcaggacg gtcgggtcac gattcggcag ttgtcagatg aaacaggtat
     1621 cagtcgtgcg tctgtcggtc gcttatgcat ggaactggtc gcaagtggtg atgtatataa
     1681 ttctggctac ggggtatttc cctctgaaca ggctcataaa gactggcaaa acgcccgcaa
     1741 aaagctatcg agggtaaagg tgaagaaacc ggttgtggtt gatcctgacc ttatctggtc
     1801 attaccagac ggagaaatac gccgctacga caggcgcctg aatataatct gtcgcgagtg
     1861 ccggaagagc gaagctatgc agcgcgtgct ggcgttttat caggggaaat ttcaggaggc
     1921 ggtactgtga gtaaaattag ctatcaggct tcaattaccg ctggtattcg catcaaagga
     1981 gaggagcatg gaaataaaac cagaagatga gttaagcaat atcgttttat ttccggtaaa
     2041 agaggatgac ccacgtaatc aggttaattt tctttatgaa ccatcggaaa gaccatattg
     2101 ccatcacgcc tctgtccggg ttgacgaaaa agagcgtcag gtccgctgta aaatctgcgg
     2161 tgcggttgtg gagccgtttg actggatgct ctctgtggca aaaagagaaa ccagactggc
     2221 agatgatgta aggctattgc gccaggagga acaggaaagg cggagaaata tagaaaagct
     2281 gatacagatt gagcgtaacg cgaaagcgcg gatacgcagg gcgacaaaat ccagaactga
     2341 ataattaaat ttagcactgt taaaaattta atccttaacc ggagggattt ctgcaccctc
     2401 aaatcatcag gagaccgccc gaaagggcgg taatgaatgg ttacattatt tagaaaaaaa
     2461 tatccgcgaa agagtagaac aacagaattc ctgtttctca ttctgtttat cgtgttgatg
     2521 acaccgatat ccccgctaat ttttgtctgg ataatcggaa aaataattga gccagttatt
     2581 gaattgtata acgacgtggt atgggcgtca ttcaacacac tgcacaataa aattaatccg
     2641 tataaggaaa gctgatatgg cactgacgaa aaaacaacgt gcagaactgc gcatgaagtt
     2701 cggtggtcgc tgcgcttatt gcggctgcga acttggcgaa aagtggcatg cagaccatgt
     2761 aaaaccggtc attcgttttg atggaaatat gcttcaccag gaacgtgacg atatatccaa
     2821 catggtacca gaatgccacc catgcaatct gcacaagcat tgcagtagcc tagaagatta
     2881 tcgacgaatt atcagtgatg gtcgtcgtga attccttgcg tccgggaaag gcaaagcgct
     2941 ggttcgtatg ggattggttg aaatgaaatc tgacccggtt gtgttctggt ttgaaaaata
     3001 tcaagaaggg gctacggcat ggccactatt accaaagagc gactgcaatg gctggctaac
     3061 atttctggcc gcgatgatat tgacgatata gacggcggtg aaattcgtga actggcgctt
     3121 atcgctctgg catcactgga agcagagcct gtagcggaat gcattgttga agatgggggc
     3181 atgtgtattg acgggttcgg tgagtatgtg ggtcactcgc tgcctgatgg aatgcatgag
     3241 ctttatgctg ccccgcgaat gcgacaacct gatggatgga aagccgtagc tgtagcgtgg
     3301 aaggtgacgt ttactcaagt tgaccaggaa tctaatacgt tcactgctat atattttgac
     3361 aaagcggaag ttgagcgttg ggtacgactg cataaagcat gtgattttcg ggcagatata
     3421 acaccgcttt acgcagcgcc ggcagtgccg gttgcagtaa acgacgacat ggcttacgca
     3481 ttccatcacg cactgtcaga ttcatcgctt ggctctggtg aaatcgaaga aattaaaacc
     3541 ggtttgcgtg ctgcctttgc caacgtcact atccaaccag agccgatagt gccggatgaa
     3601 atcgggccaa acgatagtaa tacgtttgat tatgttgatg gctggaacgc ctgccgcgct
     3661 gccatgctta agggagataa ataatgatta atcgaaccaa actggagcac attctcgaat
     3721 atgccaggca gcagaaatgt attgggcaac tttgtaaaat tccaccagga gatatggttg
     3781 aaatcgtgga aatggccatg cgtaaggctg gcaactctcc ggtaactctg gctggctgga
     3841 taagctgtag tgatgcagtt cctgctgaat attgcgatgt gattcttctc gatgatctcg
     3901 ggaatgtatt ccccggttcc tgggataagg ttttttgccc cactcgtggc gggaataaga
     3961 tggcttttgt ggacaaagac ggcgttgaag tagagagctc aactcactgg atgccgctac
     4021 cggaaccacc gcaggaggtg aatcaatgac ctggcctgag gcattcacca cggtaggaat
     4081 tgcgatggcg gtggcgctgg tggtgtattc gatttgccgt tggggttaac aaacaaaaac
     4141 cccggattga tggtccgggg tttttgaagg aaacaaaaca gaaacagcaa ttgccgttac
     4201 ctgttgttac catggcaagt aaacgtatct caggcgagcg cattgcgccg ttctgacgca
     4261 gataaattag cctggataga aggtgctggc aataaaaaat agcgttttct tatcggtatc
     4321 ggtaagattg ctgcgggtgc ttgaggctgt ctgcctcggg catgccactg taaggcagac
     4381 agagaaaagc cccagttaac attacgcgtc ctgcaagacg cttaacatta atctgaggcc
     4441 caatctatgt ctcacaaatg taggttagcc tcttacgtgc cgaaaggcaa ggagaagcag
     4501 gctatgaagc agcaaaaggc gatgttaatc gccctgatcg tcatctgttt aaccgtcata
     4561 gtgacggcac tggtaacgag gaaagacctc tgcgaggtac gaatccgaac cggccagacg
     4621 gaggtcgctg tcttcacagc ttacgaacct gaggagtaag agaccaggcg ggggagaaat
     4681 ccctcgccac ctctcatgtg tcaggcatcc tcaacgcacc cgcactaaac ccgcttcggc
     4741 gggttttttg ttgcgtgctg aatacgcagg gtgaaaaata accatatatt tgattatata
     4801 cacaacaaaa aataaaagtc attgtacttg cacattaaac aatcaaatat acggcgtgaa
     4861 ataaatattt ttcagattaa tatttttgtc tctatgtgga tataaccgtt tgtacttata
     4921 aacctggagg catcgtggag aaaataaaga aactatttag tagcaaatac gcagtcatac
     4981 gtcgtgatga cctgtcagtt atagtcgaaa tggattactt ccctgaaacc ccaaaatcaa
     5041 taatgtatcg taatggtcga aaggcaattt ttttaccgat gagggtaagt gacattatgg
     5101 gaaatgataa actgctggat gaattgcgag tcagagcatc ctgtt
//
LOCUS       DADMGC010000205         5126 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.205, whole genome shotgun sequence.
ACCESSION   DADMGC010000205 DADMGC010000000
VERSION     DADMGC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.205"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..236
                     /locus_tag="JNZ73_003953"
     CDS             <1..236
                     /locus_tag="JNZ73_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734039.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            276..816
                     /locus_tag="JNZ73_003954"
                     /pseudo
     CDS             276..816
                     /locus_tag="JNZ73_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            883..2301
                     /locus_tag="JNZ73_003955"
     CDS             883..2301
                     /locus_tag="JNZ73_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAY8734040.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2282..2752
                     /locus_tag="JNZ73_003956"
     CDS             2282..2752
                     /locus_tag="JNZ73_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HAY8734041.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="JNZ73_003957"
     CDS             complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="JNZ73_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HAY8734042.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            3834..4751
                     /locus_tag="JNZ73_003958"
     CDS             3834..4751
                     /locus_tag="JNZ73_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HAY8734043.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGTIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            4830..5012
                     /locus_tag="JNZ73_003959"
     CDS             4830..5012
                     /locus_tag="JNZ73_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HAY8734044.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
BASE COUNT         1252 a         1228 c         1462 g         1184 t
ORIGIN      
        1 gggctttcta tacatggtgt ggctatttcg tctgggcaat ggcgcgatac atctgggtga
       61 tgagccggat ccccgatgcc cccgtgtcgg gatttgaaag tgatttagga tctaccgcgg
      121 ggaagtggtt aggagcattg gtcggatttc tgtttatggc cctggttggt gccttgttag
      181 gcggcattgc atggtacacc cgacctcgtc ccgcacgttg cagacgctat gaataaaaac
      241 tgctaaggtt gcgactcttt ggcaacggag cgcgtatgga gttacaacac tggcaggcac
      301 agttcgagaa ctggttgaaa aaccatcatc aacatcagga cgcggcgcat gacctgtgtc
      361 attttcgccg tgtctgggct acggcacaga aactggcggc agatgacgat gtcgatatgc
      421 tggtgatttt aaccgcctgt tattttcacg atatcgtgag cctagcgaaa aatcatccgc
      481 aacggcagcg ttcttcaatc ctggcggcag aagagacgcg tcgcctgctc cgtgaagagt
      541 tcgtgcaatt tccggcggag aaaattgagg ccgtttgtca tgccattgcc gctcacagtt
      601 tcagcgcgca aatcgccccc ttaacaacgg aagctaaaat cgtccaggat gcggatcggc
      661 tggaagccct gggggcgatt ggcctggcgc gcgtatttgc cgtttcaggg gcattgagct
      721 tggcgctgtt tgatggcgaa gctccgtgcc gaactggcgg gggagaatga aggtatcgat
      781 cacaaggtga tagatgcgtt ttcacccgct ggctgagcgc gtggccctaa acggcagtaa
      841 ttatgttaac ctgtcggcca tctcagatgg ccggttaaat ctatgcagga aaatatatca
      901 gtaaccgatt catacagcac cgggaatgcc gcacaggcaa tgctggagaa actgctgcaa
      961 atttatgatg ttaaaatgct ggtggcgcag cttaatggtg tgggtgagaa tcactggagc
     1021 gcggcaattt taaaacgtgc gctggcgaat gactcggcat ggcaccgttt aagtgagaaa
     1081 gagtttgccc atctgcaaac gttgttaccc aaaccaccgg aacatcatcc gcattatgcg
     1141 tttcgcttta tcgatctatt tgccggaatt ggcggcatcc gtcgtggttt tgaatcgatt
     1201 ggtggacagt gcgtgtttac cagcgaatgg aacaaacatg cggtacgcac ttataaagcc
     1261 aaccattatt gcgacccggc gacgcatcat tttaatgaag atatccgcga catcaccctc
     1321 agtcatcaag aaggcgtgag tgatgaggcg gcggcggaac atattcgtca acacattcct
     1381 gaacacgatg ttttactggc cggtttccct tgtcagccat tttcgctggc tggcgtatcg
     1441 aaaaagaact cgctcgggcg ggcgcacggt tttgcctgcg atacccaggg cacgctgttc
     1501 tttgatgtgg tgcgcattat cgacgcgcgt cgtccggcga tgtttgtgct cgaaaacgtc
     1561 aaaaacctga agagtcacga taagggaaaa acgttccgca tcatcatgca gacgctggac
     1621 gaactgggct atgacgttgc cgatgcagaa gataacgggc cggacgatcc gaaaatcatc
     1681 gatggtaaac atttcctgcc gcagcaccgt gaacgcatcg tgctggtggg ttttcgtcgc
     1741 gatctgaatc tgaaagccga ttttactctg cgtgatatca gcgaatgttt ccctgcacag
     1801 cgagtgacgc tggcgcagct gctggacccg atggttgagg cgaaatatat cctgacgccg
     1861 gtgctgtgga agtacctcta tcgatatgcg aaaaaacatc aggcgcgcgg taacggcttc
     1921 ggttatggaa tggtttatcc gaacaatccg caaagcgtca cccgtacgct gtctgctcgt
     1981 tattacaaag atggcgcgga aattttaatc gatcgcggtt gggatatggc cacgggtgag
     2041 aaagactttg acgatccgct gaatcagcaa catcgtccac gtcggttaac gcctcgtgaa
     2101 tgcgcgcgct taatgggttt tgaagcgccg ggagaagcga aattccgcat tccggtttcg
     2161 gacactcagg cctatcgcca gttcggcaac tcggtggtcg tgccggtctt tgccgcggtg
     2221 gcaaaactgc ttgagccaaa aatcaaacag gcggtggcgt tgcgtcagca agaggcacaa
     2281 catggtcgac gttcacgata aggccacccg cagcaaaaat atgcgcgcga ttgccacgcg
     2341 tgatacggcg attgagaagc gcctcgccag tttgttaacc gggcagggcc ttgcgtttcg
     2401 cgttcaggac gccagtctgc ccggacgccc ggattttgtc gttgatgaat atcgctgcgt
     2461 gatatttacc cacggctgct tctggcatca tcatcactgc tatctgttta aagtgcctgc
     2521 gactcgaact gagttctggc tggagaagat aggtaaaaat gttgaacgcg atcgccgcga
     2581 tatcagtcgc ttgcaggagc tcggctggcg cgtactgatt gtctgggagt gtgcgttacg
     2641 taggcgcgag aagctgacgg atgcggcgct taccgagcgt ctggaagagt ggatctgcgg
     2701 cgaaggtgcc agcgcgcaga tcgacacgca ggggattcat ttactcgctt gatgcatcct
     2761 gaataactgg cgcgacaacg ggtttggccg ggaagagata ttttcccaac gtgaccagta
     2821 ccaccgcgaa aacaattacg ccgagcgcca gccattcaat cttcgacagc gtttcgccac
     2881 ccagtcccgt acccagcaag accgcgacca ccgggttaac gtaggcgtag ctggtggcga
     2941 gagccggact gacattacgg attaaataca tataagcgtt aatggcgata atcgaaccaa
     3001 acagcgccag atagccgacc gcaaggaagc ctgacaggga agggagcgcc gtcagttttt
     3061 cacccgcaat catcgatgcg atcattaaca ccacgcctgc cgccagcatc tcaatcgcac
     3121 ccgccatcat ccctacaggt aaggtaatgc gcgagccata aactgagcca aacgcccagc
     3181 taatcgagcc gattaaaatc agaatcgcgc cccacggatt gccgcttaaa tttccaccgc
     3241 tattgagcat gatgattccg gcaagcccaa tggcaatacc cacccattcc agtttgcgcg
     3301 ttttaatgcc aaacaggcga ctgaagcaca gggtaaagag gggcacggtt gcaatcacta
     3361 cggcggcgat gccggaagga acattttgat gttcggcaac cgtcaccatg ccattaccga
     3421 cagccagcaa taacaggcca atcagcgcgg cattgagcag cggacgtagc gggggaagtt
     3481 tgtgtccgcg cagtagcaaa aatgccagca ataaaatacc ggctgccagg aatcgaacgc
     3541 ccgccatcat taacggaggc cagcttccca cgccaatccg aatgacaaaa taggttgagc
     3601 cccaaatgat atacaacgca aacagcgcgc caaaaagcgg taacaactgg cggaaacgca
     3661 taatccctca cggcggaaat aaaaaggtgg ttcatagtaa acgtgaaaat cattctgctg
     3721 gctagagata taattgcact tgattgttaa ataaatgttg acctgtgaag caagtatcag
     3781 agctgccgtt tttgcttcat acttacaccc ttcagcaata aaaatgagag ggaatgcttt
     3841 tggccgggag tagtttactg acgttgctcg atgatatcgc cacactgctg gacgatatct
     3901 ctgtgatggg caaactggcg gcgaagaaaa ccgccggtgt attaggggat gatttatcgc
     3961 tcaatgcgca acaagtttca ggcgtgcggg ccaaccggga acttcccgtg gtctggggcg
     4021 tggcgaaagg atcgctgatc aataaagtga ttctggtgcc gctggcgctg atcatcagtg
     4081 cgtttatccc gtgggcgatt acgccactgt tgatgattgg tggcgcgttt ctctgctttg
     4141 aaggggtaga gaaagtgctg catatgctgg aggcgcgtaa acataaagaa gatccagcgc
     4201 agagccagca gcgtctggag aagctggcgg cgcaggatcc gctgaagttt gaaaaggaca
     4261 aaattaaagg gacgattcgt accgatttta ttttgtctgc ggaaatcgtc gccatcacgc
     4321 tggggattgt ggcagaggcg ccgttgctta atcaggtgct ggtgctttca ggcatcgcgc
     4381 tggtagtgac cgtgggcgtc tacggtctgg taggggttat cgttaagatt gatgacctag
     4441 ggtattggct ggcggaaaaa tccagcgcgc tgatgcaggc attaggtaaa gggttattga
     4501 ttatcgcgcc ttggctgatg aaagcattat cgattgtcgg cacgctggcg atgttcctcg
     4561 tcggcggcgg gattgtggta catggtattg cgccgctgca tcacgctatt gaacatatcg
     4621 ccgggcagca aagtgcagtg gtggcgatga tattaccgac tgttttaaat ctgattcttg
     4681 gatttatcat cggcggcatc gtggtgctgg gagtgaaagc agtagcgaaa atgcgcggtc
     4741 aggcacatta atactcgcac tggctattat gcaaaattcg ccatcttcaa ctaaggtgaa
     4801 caagtttcac tcgaaaaaag gaggcgagta tgagctttat ggttagtgag gaagttacgg
     4861 ttaaagaggg cggcccgcga atgattgtca ctggatactc cagcggtatg gttgagtgtc
     4921 gttggtatga cggttacggg gtcaagcggg aagcttttca tgaaaccgat cttgttccgg
     4981 gggaggggag tcgttctgcg gaagaagttt gaaagggtat cattaacacc aggccggatc
     5041 acatccggcc tggtgtccca atgcctgcaa tgaatgatag tcgcgtctta tcatgccacc
     5101 attataaaat tatcgctgtt ttgcgg
//
LOCUS       DADMGC010000206         4992 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.206, whole genome shotgun sequence.
ACCESSION   DADMGC010000206 DADMGC010000000
VERSION     DADMGC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.206"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            91..1206
                     /gene="adrA"
                     /locus_tag="JNZ73_003960"
                     /gene_synonym="dgcC"
     CDS             91..1206
                     /gene="adrA"
                     /locus_tag="JNZ73_003960"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HAY8734045.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1223..2032)
                     /gene="proC"
                     /locus_tag="JNZ73_003961"
     CDS             complement(1223..2032)
                     /gene="proC"
                     /locus_tag="JNZ73_003961"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAY8734046.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRVQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2152..2610
                     /locus_tag="JNZ73_003962"
     CDS             2152..2610
                     /locus_tag="JNZ73_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAY8734047.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2793..3317
                     /gene="aroL"
                     /locus_tag="JNZ73_003963"
     CDS             2793..3317
                     /gene="aroL"
                     /locus_tag="JNZ73_003963"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HAY8734048.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPNQV
                     ISEIRSALAQTINC"
     gene            3367..3558
                     /gene="yaiA"
                     /locus_tag="JNZ73_003964"
     CDS             3367..3558
                     /gene="yaiA"
                     /locus_tag="JNZ73_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HAY8734049.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            3816..4493
                     /locus_tag="JNZ73_003965"
     CDS             3816..4493
                     /locus_tag="JNZ73_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HAY8734050.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLEKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            4565..4849
                     /gene="ppnP"
                     /locus_tag="JNZ73_003966"
     CDS             4565..4849
                     /gene="ppnP"
                     /locus_tag="JNZ73_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAY8734051.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT         1213 a         1186 c         1316 g         1277 t
ORIGIN      
        1 gctaacgggt gagctacgaa agtggctcac ccgaaatttc atacttctgc ctttagcccc
       61 gtctctataa tttgggaaaa ttgtttctga gtgttcccaa aaataatgaa tgatgaaaac
      121 tttttcaaaa aagcggcggc gcatggggag gaacctcctt taactcctca aaacgaacat
      181 cagcggtccg ggctgcgctt cgcccgtcgc gtcagactac cccgtgcggt tggcctggct
      241 ggcatgttct taccgattgc ttcaacgctg gtttcgcacc cgccgccggg ctggtggtgg
      301 ctggtgttgg tcggctgggc gttcgtctgg ccgcatttag cctggcagat agcgagcagg
      361 gccgtcgatc cgcttagccg ggaaatttac aacttaaaaa ccgatgcagt attagcggga
      421 atgtgggtag gcgtaatggg cgtaaacgtg ctgccttcca ccgctatgtt gatgattctg
      481 tgtctgaatt tgatgggggc aggcggcccc cgtctgtttg tcgcgggtct ggtgttgatg
      541 gtggtttcct gccttgtcac cctcgagctt acgggcatta ccgtgtcgtt caatagtgcg
      601 ccgctggaat ggtggctctc ccttcccatt attgtcgttt atcctctgct gtttggctgg
      661 gtcagctacc agacggcaac caaactggcg gaacataaac gcaggttgca ggtcatgagt
      721 actcgcgacg gcatgacggg cgtgtataac cgacgtcatt gggaaactat gttacgcaat
      781 gaatttgata actgtcggcg gcataatcgc gatgcaacgt tactgattat cgatatcgac
      841 catttcaaga gcatcaacga tacctggggt catgatgtgg gcgatgaagc gattgtggcg
      901 cttacccgac agttacaaat taccctgcgc ggtagcgatg tgattggtcg gtttggcggc
      961 gatgagtttg cagtaatcat gtccggtacg ccagctgaga gcgccattac cgccatgtta
     1021 cgggtgcgtg aagggctaaa cacgttacgc ttgccgaata cgccacaggt aactttacgg
     1081 attagtgtgg gggttgcgcc gctgaaccca caaatgagtc actatcgtga gtggttgaaa
     1141 tcggcagatt tggcgcttta caaagcaaag aaagccggac gtaaccgcac cgaagtggcg
     1201 gcctgacgtc cggcgaaagt catcaggatt tgctgagttt ttctgatttt tccatacact
     1261 tcgtcatcgc ttcgatcact gcggcacgga agcctttctc ttccagtacg cgtaccgctt
     1321 caatggtggt acctcccggt gagcagacca tatctttcag tgcccccgga tgttctcccg
     1381 tttccagcac catttttgcg gaacccatta ccgcctgagc ggcaaattta tacgcctgga
     1441 cgcgtggcat cccgcccagc acggcggcgt cggccatcgc ttcgataaac ataaatacgt
     1501 aggctggcga agaaccgctc acaccgacca ccgggtggat catcggctca gcaattactt
     1561 ccgcttcgcc aaagcagcgg aaaatattca gcacatcagc ggtatcttct ggggttacca
     1621 gcgcgtttgg cgttacggag gtcatcccgg cattaaccag cgcgggagtg ttcggcatgg
     1681 cgcggataat tttccggtca tggcccagcg cgcgggcaag ctggtcgagc gtgacacctg
     1741 cagcaataga aacgaccaga gagtctttat tcaggctgga ggtgatttcg ctaagcactt
     1801 taatcatgat gccaggttta acggcagcaa aaatgatgtc ggcgatttgc gccacttctt
     1861 gcgccgattc tgcggcgttg atgccgaact ggtcatgcag ggcggcgact ttatccgggg
     1921 agggggtgta tacccagatt tgccctggaa gcacctgacc gctggcaatt agaccgccga
     1981 gaatggcttt tcccatattg ccgcagccaa taaaaccgat tttcttttcc attgcctcac
     2041 tcctgccgtg aaattcattg ttttgataat cgctggcaga agcataaaca gaactatgcc
     2101 ggaaggcaaa agcgcgacac aatagaggat tacccaacaa aggatgactt tatgacaatt
     2161 tgggtggatg ccgacgcgtg tcccaatgta attaaagaga ttttgtatcg cgcggcggaa
     2221 cgtatgcaga tgccgctggt actggtagca aaccagagtt tacgcgtgcc gccatcgcga
     2281 tttattcgta cgctgcgcgt cgcggcaggt ttcgacgttg ccgataacga aattgtccgg
     2341 cagtgtgaag cgggcgattt ggtgatcacc gcagatatac ctttggctgc tgaagccatc
     2401 gagaaaggcg cagcggcgct taatccgcgc ggcgaacgtt acacgccagc gaccattcgt
     2461 gagcgcctga cgatgcgcga ttttatggat accttacgtg ccagtgggat ccagaccggc
     2521 ggaccagata gcctttcaca acgtgaccgc caggcctttg ccgcggagct ggagaagtgg
     2581 tggctggaag tgcaacgtag tcgtggctaa atgtaattta ttatttacac ttcattcttg
     2641 aatatttatt ggtatagtaa gggatgtatt gagattttca ctttaagtgg aattttttct
     2701 ttacaatcga aattgtacta gtttgatggt atgatcgcta ttctcatgac accggctttc
     2761 gccgcattgc gacctattgg ggaaaaccca cgatgacaca acctcttttt ctgatcgggc
     2821 ctcggggctg tggtaaaaca acggtcggaa tggcccttgc cgattcgctt aaccgtcggt
     2881 ttgtcgatac cgatcagtgg ttgcaatcac agctcaatat gacggtcgcg gagatcgtcg
     2941 aaagggaaga gtgggcggga tttcgcgcca gagaaacggc ggcgctggaa gcggtaactg
     3001 cgccatccac cgttatcgct acaggcggcg gcattattct gacggaattt aatcgtcact
     3061 tcatgcaaaa taacgggatc gtggtttatt tgtgtgcgcc agtatcagtc ctggttaacc
     3121 gactgcaagc tgcaccggaa gaagatttac ggccaacctt aacgggaaaa ccgctgagcg
     3181 aagaagttca ggaagtgctg gaagaacgcg atgcgctata tcgcgaagtt gcgcatatta
     3241 tcatcgacgc aacaaacgaa cccaaccagg tgatttctga aattcgtagc gccctggcac
     3301 agacgatcaa ttgttgattt tagagcgcct atacttaacg ttcatcccgt gaaataagga
     3361 agaacgatgc caacgaaacc gccttatcct cgtgaagcat atatagtgac gattgaaaaa
     3421 ggaaagccag gacagacggt aacctggtac caactcagag ccgatcatcc taaaccagac
     3481 tcgttgatca gtgaacatcc gacggctcag gaagcgatgg atgcgaaaaa acgctatgag
     3541 gaccctgaca aagagtgacc gcatcagact gctcggaagg gattctgagt gccactacaa
     3601 gggatctgcg tcacattttt cataattcat gtttttctaa taattagaat attaaacaat
     3661 aacattccat tactggaatc atttggaatc tttacattat gccgtgcacg tctgctgcta
     3721 cgctttttgt catttgtagc acaagtaagt gtcagcagtg gtgcttcaca cttgcccggt
     3781 aattaacgac gaaagaaaag taaggtggat gaacaatgag tgcgtcgttg gcgatcctga
     3841 ccatcggcat tgtacctatg caggaggttt tgccgctcct gacggaatac attgacgaag
     3901 ataatatttc ccatcatagc ctgctggaga agttaagtcg tgaagaagtg atggcggagt
     3961 acgcgccaga agcaggcgaa gacaccattc tcacattatt aaatgacaac cagctggccc
     4021 atgtttcgcg tcgtaaagtg gagcgagatc tgcaaggtgt ggttgaagtg ctcgataatc
     4081 agggttatga cgtcatttta ttaatgagta cagcaaacat tagtagtatg actgcgcgta
     4141 atacgatctt tcttgagccg tcgcgaatat tgcctccact ggtttcctct attgttgaag
     4201 atcatcaggt gggggttatc gttccggttg aggagatgct gcccgttcag gcgcaaaaat
     4261 ggcaaatttt gcagaaatcg ccggtatttt cattgggtaa ccccattcat gattcagaac
     4321 aaaaaatcat tgatgctggg aaagaattac tggcaaaagg ggctgatgtc atcatgctgg
     4381 attgtttagg attccaccaa cgtcatcgcg atttactgca aaaacagctc gatgttcctg
     4441 tcttgctctc taacgtattg attgcacggc tggctgcgga attactggtg taattttgcg
     4501 tgacagccag cgcctctggc ccctatagtg aagtagatgt tcaactacca gacagggcca
     4561 gtttatgctt caaagtaatg agtacttttc cggcaaagtg aaatcgatcg gcttttccag
     4621 cagcagcact ggtcgcgcca gcgtgggtgt tatggttgaa ggcgaataca ccttcagcac
     4681 cgctgagccg gaagagatga cggtaatcag tggcgcgctg aatgtgttac tgcctgacgc
     4741 gaccgactgg caagtgtatg aagccggttc ggtgtttaat gttcccggtc acagtgagtt
     4801 tcatcttcaa gttgccgaac ctacctctta tctgtgccgc tatctgtaat tcctcgcctt
     4861 ccccttgaac gggagggcat ttttctgaaa tatcctctct ttaacccata ataatatttc
     4921 ctttgctgcg attttttcaa tttccgatat attcataatt catcaaggtt gatataaata
     4981 tcagtgaaga tc
//
LOCUS       DADMGC010000207         4984 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.207, whole genome shotgun sequence.
ACCESSION   DADMGC010000207 DADMGC010000000
VERSION     DADMGC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.207"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..181)
                     /locus_tag="JNZ73_003967"
     CDS             complement(<1..181)
                     /locus_tag="JNZ73_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HAY8734052.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGL"
     gene            complement(245..2074)
                     /gene="recQ"
                     /locus_tag="JNZ73_003968"
     CDS             complement(245..2074)
                     /gene="recQ"
                     /locus_tag="JNZ73_003968"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAY8734053.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLLDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(2207..3076)
                     /gene="pldA"
                     /locus_tag="JNZ73_003969"
     CDS             complement(2207..3076)
                     /gene="pldA"
                     /locus_tag="JNZ73_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HAY8734054.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3241..3708
                     /locus_tag="JNZ73_003970"
     CDS             3241..3708
                     /locus_tag="JNZ73_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HAY8734055.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3760..4668
                     /gene="rarD"
                     /locus_tag="JNZ73_003971"
     CDS             3760..4668
                     /gene="rarD"
                     /locus_tag="JNZ73_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HAY8734056.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSKSKINIF"
     gene            4709..>4984
                     /locus_tag="JNZ73_003972"
     CDS             4709..>4984
                     /locus_tag="JNZ73_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734057.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRRRYIFSFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTL"
BASE COUNT         1186 a         1286 c         1241 g         1271 t
ORIGIN      
        1 gcaggccaag caacgcaatc ccagcccaaa ccattacgcc gcaggtaatg cccagcacgc
       61 ccatcatcgc ttctttacgg gaacgactga cagcggtctg agagacaaaa aagaaatcgg
      121 gaccggggct cataagcgcc acaatgtgca ccatggcgac ggtgagaaat aacatcaaca
      181 tacatttgac tcgcggggga aacgtttacg gagtcttcat actggcactt ttttatgctg
      241 ctgactactc ttcgtcgtcg ccatcgacat gcgcacgaat cagcgccata aacggtttac
      301 caaagcgttc cagcttgcgc atcccaacgc cgttaacgct gagcatttcg ctggcggtga
      361 tcggcatctg ttcagccatc tcaatcaagg ttgcgtcgtt aaacaccacg tacggcggga
      421 cattactttc atcggcaatc gatttacgca gtttgcgtaa tttggcgaac agtttgcgat
      481 catagttgcc gccgaacgat ttctgcatcg ctttcggttt gagcgccacg atacgcggca
      541 cggcaagttg caaagaggat tcgccgcgca gcaccgggcg cgcggcctct gtcagttgta
      601 gggcagaatg ctgggcaata ttttgcgtca ccaggccgag atgaatcagc tggcggatca
      661 cgctcaccca atgttcatgg cttttatcac ggcccatacc atagactttc agtttgtcat
      721 gaccatagtc gcggatacgc tggttattag caccacgaat cacttccacc acataaccca
      781 tcccaaaccg ctgattcaca cgaccaatgg tggaaagggc aatctgagca tcggttgaac
      841 cgtcgtactg tttcggcgga tcgaggcaga tatcgcagtt cccgcacggc tcctgacgcc
      901 cttcgccaaa atagttcagc aataccagac gacggcaagt ttgcgcttcg gcaaacgcgc
      961 ccatcgcatt gagtttgtgg cgctcgatat ccagcaactg cccctgcggc ttctcttcca
     1021 gacaacggcg cagccacgcc atatcagccg gatcgtaaaa cagcatcgct tccgcaggca
     1081 ggccatcacg cccggcgcga ccggtttcct gataatagga ttcgatattg cgcggaatat
     1141 caaagtggac cacgaagcga acgtttggtt tattgatgcc catgccgaac gccaccgtcg
     1201 ccaccacaat ttgcagatca tcgcgctgga atttctcctg cacgtcggcg cggacattat
     1261 tttccagccc ggcatgatag gccgccgcgc taataccctt gctttgcagg cgcgcagcgg
     1321 tgtcttctac tttcgcccgg ctgttgcagt agataatgcc agacttaccg cgctgttcct
     1381 gcacgtagcg catcaactga tcgagcggtt tgaatttctc catcagcatg tagcgaatat
     1441 tcggacggtc aaaactgctg atttgaatca gcggatcgtt cagccccagc aggcgcacga
     1501 tatcctggcg cgtggtgtcg tcggctgtgg cggtcagcgc cataaacggc agcgtcggga
     1561 accgctggcg caactgaccg agcgcggcat attccgggcg gaaatcgtgg ccccattggg
     1621 agatacagtg cgcttcatcg acggctaata acaccggatt ccagtgcgcc agatgctcaa
     1681 gaaagttatc cagcatcagg cgttccgggg cgatatacag taagcgaatt tgcccggtgc
     1741 ggcagcctgt catcacttca agttgctgtt cgcgggtttg cgtcgagtta aggcacgccg
     1801 ccgccacgcc gttggcctgt agttgatcca cctgatcttt catcaacgaa atcagcggtg
     1861 aaacaaccac ggtaaggccg tttagcaata aggcaggaat ttgatagcaa agggattttc
     1921 cgccaccagt gggcataaca accaggcaat cgcgaccgga aagcacagtg tcgataattt
     1981 cttcctggcc ggggcgaaac tgttggtagc caaaggtttc ttgtaaaacc tgtttagctc
     2041 cggactccag attcaacact tccgcctgcg ccacattcac cccgattgcc tgaaataaaa
     2101 acaggcgcta ttttcagcgc ctgcgcgaga aactgcaatg cttttagtct ctgagagggt
     2161 aatcctggat gctggattcc agatttgcca accactcaac cgttcatcaa aacaagtcgt
     2221 ttagcataac ccctacaccg acacgggtct ggttgaagtt atagtcgatg agcgattctc
     2281 catagccgct gtaaacctga gtataaaggc gcacatgttt ggtgatcggg taacttaagc
     2341 ctaactccgc gccgccgtag ccggtgttcc agttgtactg tcctttcgca ctgagcaccg
     2401 catcaccgag gtgatagccg attttaagct ggtagtaacc catatatttg gtgatatccg
     2461 ggttatcgtc agtattaccc accacatacc acggcttcac ttctaccagc cagttaccgt
     2521 tttctgccat caggcgagta taaaggcggt tccagctgcg ggaggtcggg tctgaacgcc
     2581 cgttagagtc gtggttatac cccatctcca catcgcgcag cgtccaacct gcaaaacggt
     2641 aatcggtggc aaaaccgagg aacaattgcg gttcgtagtt ggtttcacga aacggtgaag
     2701 actcttcgct attggacagt tgccaccagg atttttgcgt ataagacgca cccaacaccg
     2761 agtttgggcc taaaatccca cgccacagcg gaaatgccag gctcaactga aactttactt
     2821 catccttacg cgcattttcc gcccagtcgt aactggcaat cgcttcttta ttcagatcgc
     2881 tggtttgggt gtagatgagg tagttggtgt cataaggata gagcgtgaac ggattgtcat
     2941 gctcctgcag catattggcg ataatactgc cacgcaccgc tggcgcgtca tgcacctctt
     3001 tcaccgttgc ctcttgtgca tatactgcca taggcaacat aaacaccggc aacaaccagc
     3061 cctgcagagt ccgcatcggt cgtgttctcc gtaacgaatt attgataagg tgaatgtttt
     3121 tctgcgggct attttacata cttaactcca ttgccgttag ttatcccttc ctaaagtaga
     3181 aaccaacaac aaagaagcat aaaatcaaca tttcattaac caatgaagtg taaggatgat
     3241 atgtctgccg tactgaccgc tgaacaagcc ctgaaattag tgggtgagat gtttgtttat
     3301 cacatgccat ttaaccgcgc attggggatg gaactggagc gttacgaaaa agagttcgca
     3361 cagctggcct ttaaaaatca gccgatgatg gtgggtaact gggcgcaaag cattttgcac
     3421 ggcggagtca ttgcgtcggc gctggatgtc gccgccggtc tggtgtgcgt gggaagtacc
     3481 ttaacccgcc acgaaaccat cagtgaagat gaactacgcc agcggctatc gcggatgggg
     3541 accattgatc ttcgcgttga ttatctgcgc ccaggcagag gcgagcgttt tactgctacc
     3601 agtagcctgt tgcgtgcagg caataaagtc gccgtcgccc gcgttgaatt acacaatgaa
     3661 gaacagcttt atattgccag tgccaccgcc acctatatgg taggttgaag acataaaagc
     3721 aggtaaactg ctgttaccaa ttaaaaatga gtattcccga tggatgcaaa acaaacgcgg
     3781 cagggcgtat tactcgctct tgccgcttat tttatttggg gtattgcgcc agcatacttc
     3841 aagctgattt actacgtgcc cgccgatgag atcctgacgc atcgcgtgat ctggtcgttt
     3901 ttctttatgg tggtgctgat gagcatttgc cgccagtggt cctatttaaa aacgctgatt
     3961 cagacgccac agaaaatttt tatgctggca gtctctgccg tgctgattgg tggcaactgg
     4021 ctactgttta tctgggcggt gaacaatcac catatgctgg aagcgagcct tggttacttt
     4081 attaacccgc tggtgaacat tgtgctgggg atgattttcc tcggcgagcg attccgccgg
     4141 atgcaatggc tggcggtgat tctggcgata tgtggcgtgt tggtccagct gtggactttt
     4201 ggttcgctac ctattatcgc gctggggctg gcatttagtt ttgccttcta cggcctggtg
     4261 cgcaagaaga ttgcggtaga agcacaaacc ggcatgttaa ttgaaaccat gtggctgctg
     4321 cccgtggcgg cgatttacct gtttgctatt gccgacagtt caaccagcca tatggggcaa
     4381 aacccgatgt cgctgaattt actgctgatc gccgccggta ttgtcactac cgtaccgctg
     4441 ttgtgtttta ctgccgctgc cacgcgcctg cgtctctcaa cgttaggctt ttttcagtac
     4501 attggcccga cgctgatgtt cctgctggct gtgacgtttt atggtgaaaa accgggtgcc
     4561 gataagatgg tgactttcac ctttatttgg gtggcgctgg caatttttgt gatggacgcg
     4621 atttatacgc agcgtagaac gtccaaatca aaaataaata tattctgaaa aaatatgccg
     4681 actcttgtat gagagtgggc atattgccat gctgacagga gactcacaca aggatgtaaa
     4741 atttatgtta cgtatgttta tcccaacatc taacggtaag atttccagac gccgatatat
     4801 attttcattt attttaataa atttcatttt tgcctttctt attatctttt ttaacgatgg
     4861 ggaagcaggt tttcttgtta tagtctctac catcgcactc cattatctcg tcatcaacat
     4921 gaattgccaa cgactccgtg atagtggttt catatatata aaaatttatg tttttggtac
     4981 atta
//
LOCUS       DADMGC010000208         4968 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.208, whole genome shotgun sequence.
ACCESSION   DADMGC010000208 DADMGC010000000
VERSION     DADMGC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.208"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..527
                     /locus_tag="JNZ73_003973"
     CDS             <1..527
                     /locus_tag="JNZ73_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734058.1"
                     /translation="ACDILQKKQSDIPKRIKGYIRQAFGDVSFGYALLEDVVSKLKRG
                     KFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGFALKIYGGDERFRNGSSVVLED
                     EDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKICEKYKSIIEAFKKEQ
                     LALPVLVRCKNSET"
     gene            complement(590..1144)
                     /locus_tag="JNZ73_003974"
     CDS             complement(590..1144)
                     /locus_tag="JNZ73_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY8734059.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPRSQFIVDSNGVARGAWQLDDESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            1469..1675
                     /locus_tag="JNZ73_003975"
     CDS             1469..1675
                     /locus_tag="JNZ73_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HAY8734060.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(1737..3080)
                     /locus_tag="JNZ73_003976"
     CDS             complement(1737..3080)
                     /locus_tag="JNZ73_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY8734061.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(3403..4041)
                     /gene="msrA"
                     /locus_tag="JNZ73_003977"
     CDS             complement(3403..4041)
                     /gene="msrA"
                     /locus_tag="JNZ73_003977"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAY8734062.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGLYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            4247..>4968
                     /gene="tamA"
                     /locus_tag="JNZ73_003978"
     CDS             4247..>4968
                     /gene="tamA"
                     /locus_tag="JNZ73_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HAY8734063.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVV"
BASE COUNT         1303 a         1137 c         1249 g         1279 t
ORIGIN      
        1 atgcctgtga tattttgcag aaaaaacagt ctgacatacc aaaaaggatt aaaggttata
       61 ttcgccaggc gtttggtgac gtatctttcg gttatgcctt attggaggat gtagtaagca
      121 aattaaaacg gggtaaattt gaactccaaa taccaggcgg tggaattaaa gaatgtgatg
      181 gatggtatat ttacgaaaaa ataattgatg ataattttgc catagtcata gaatctttgg
      241 gctttgcgct taaaatctat ggaggggatg aacgttttcg taatggttcc tctgttgttt
      301 tggaggatga agattactct cttatttata attttctggt taatgcaggt tgccaacaag
      361 tcgaattagc cgaacaagtg gatgcgatag taagtgcgaa tctggccgca gatagtaata
      421 ttaccaaaga aaagatttgc gagaaatata aatcgatcat tgaggcgttt aaaaaagagc
      481 aactagcgtt accagtatta gttcggtgta agaactcaga gacttaattc ccctatccca
      541 tagataacga taggggaaaa ttatttttcc agaatttatg ttattgccat tatttgagca
      601 acttttgcag caggtccatc acttgctgca cctcttccgg tgtgagcgcc ccgtctttgg
      661 cccattgcac gcgaccgtct ttatccagca ccactacagc ggaactttcg tcatccagct
      721 gccaggcacc gcgcgcgacg ccattgctat cgacaataaa ctgcgacctg ggataaagct
      781 ttttattact ctccagactg ctgcgcacaa acatgccgga acccggaatc gcgtcgtcgg
      841 tgttaacaat ggtggtggtc tggtaacgat cgtgcggtaa cttcgctgat ttaatcgctt
      901 caatcagcgt cgcgtttttc tcttttgcag aggtgcgacc agcaatatgt tgcagtactc
      961 gcacttttcc cactaactgc gcgctgttcc aggttttgta gctaaactga tctttatcaa
     1021 gcaccaactc gccccgatcg gtaatgccaa tcggcggcac tcgctgaccg atttcgaact
     1081 gatgtgcgga agccatcatc ggcaacagca ggcaggtgag tgccagaatc ttgcgtaggg
     1141 tcatggtgtt tccttcttat gatatgcagg tgatccgacc acttgggtct gagttttaat
     1201 catatgtgct atttatcgaa tttcccgcaa gtgtgatgcc agtttgcggt caagcgcaca
     1261 aatcatatga aaaatgaatg cttatactga agaccgcgct tcggtaaaaa gataattctg
     1321 aataattgta acctttaggt aaaaaaagtt atacgcggtg gaaacattgc ccggatagtc
     1381 tatagtcact aagcattaaa atttgcgcct cataatagtt gggccgattg tggcaccgca
     1441 caggcgtaat actcagcagg agataacaat gaaaattttc caacggtaca acccacttca
     1501 ggtggcgaag tacgtaaaga tcctgttccg tggacggtta tacatcaagg acgttggcgc
     1561 ttttgaattt gataagggta agattcttat cccaaaagtg aaggataagc tgcatttgtc
     1621 agtgatgtcc gaagttaacc gtcaggttat gcgtctgcaa acagagatgg cttaaccaaa
     1681 gtgctatgca gtaataagac ggctcctgat tcaggagccg ttgatgtttc tgggggttac
     1741 gcgacgcttt cttctttatc tttagcgtca ggcagttttg gcgaaagggc ggtggccttg
     1801 ctgtcgatcc gggtcaccag cagctggtcg atgcggtagt tatcgatatc caccacttca
     1861 aatttgtagc cggcgaattt caccgaatcg gtgcgtttcg ggatcttacg cagcataaac
     1921 atcataaagc cgccgatggt ttcgtagttg cccgactgcg ggaactcgtc aatatccagc
     1981 acgcgcatga cgtcgtcaat tggggtgccg ccgtcaatca gccatgagtt ctcatcacgg
     2041 gcgacaatct gctcttccag cccctgaccg accagatcgc ccatcagcgt ggtcatcacg
     2101 tcgttgaggg tgatgatccc caccaccagc gcgtactcgt tcatgatcac cgcgaagtct
     2161 tcacctgcgg ttttaaaact ttccaacgcc tctgaaaggg ttaacgtatc cggcacaatc
     2221 agcgtgttgc gaatttgtac gccgctgttc agtgccaggc tttggttagc cagcacgcgg
     2281 ttcagcaggt ctttagaatc gacataaccg atgatgtggt caatatcttc attacagacg
     2341 aggaacttag agtgcggatg ttccgccacc ttattcttca ggctttgctc atcttcgtgg
     2401 agatcaaacc aaatcacgtt ttcacgcggt gtcattgaag acggaacggt acgggattcc
     2461 agctcaaaga cgttttcaat cagctcgtgt tcctgtttac gtaacacgcc cgccagcgca
     2521 ccggcttcca ctaccgcgta gatgtcatca gaagtgatgt catctttacg taccattggc
     2581 agtttgaaaa tacggaagat catgttcgcc aggccgttga agaaccacac cagcggggtg
     2641 caaacgtaca ggcagaagcg catcgggttg atgatacgca aagccaccgc ttctggcgca
     2701 atcataccga tgcgtttcgg ggttaaatcc gcaaacagga taaacatgcc agtcactaac
     2761 gagaaagaga gaataaagct cagttgctca gagagctctg ccgacatata gcgggagaac
     2821 aggctgtgaa aagctggaga aaatgccgca tcaccgacga taccgccgag aatcgccact
     2881 gcgttcagac cgatttggac cacggtaaag aacatgccgg gattttcctg catattcaga
     2941 acgcgttggg catttatatt gccttcatca gccagcagtt taagtttgat tttgcgtgag
     3001 gcggcaagcg agatctcgga catcgagaag aacgcactta cagcgatcaa gcagagtatg
     3061 actaaaatac tgtttaacat atcttatccg acctttcagg ccagatcctc ggaagggaaa
     3121 gttgattaat ttgtgtgaaa tacacattga aagccgattc gaagaaagtg aaccggcatt
     3181 ttcagtcgct agtatagcgt aaggtactgt aaagtcgcca gagggttaaa attcagactg
     3241 ctgataaatg caatactgcc tgatgcgcta tgccgatcag gcctacgcag tccttgcaat
     3301 atattgaatt tggacgattt tgtaggcctg gtaagtcgtt taagccgcat caggcataga
     3361 caaagagcac tttatcaaca aactacattt gtacccgtaa cgctatgctt ccggcggcag
     3421 acagacgcca attccgccaa ttccacagta accatatggg tttttatgca gatattgctg
     3481 gtggtcatct tcggcataat aaaacggtgt ggcgttagcg atttccgtgg tgatgtgacg
     3541 atcgtcatcg gcggcaagca tcgccgcctg aaaacgttcc agactggcgc gagctgcggc
     3601 atcctgttct ggggtcagcg gataaatcgc tgaacgatac tgcgtgccgt ggtcattgcc
     3661 ctgacgcatt ccctgggcgg gatcgtgatt ctcccaaaat acctgtagca actgctcata
     3721 gctgatgacg gaaggatcgt aaacaatgcg taccgcttcg gcatgacccg tatcaccgga
     3781 gcacacttcc cgataagtcg gatttggcgt atagccgccg gtatagcctg cggcggtgct
     3841 gtaaaggccg ggtaactgcc agaacagacg ctccacaccc cagaaacaac ccatcgcaaa
     3901 aatggcaatc tccattccgt caggtacatt ggtcattgag tgaccgttga ccgcatgcag
     3961 cgtggctacg ggcatcgggg tgttacgtcc aggcagggca tcggcggggg aaaccagatg
     4021 ctttttatca aataaactca tggtgtcgct ctcccgaaaa tcggtcattg gggttaaggt
     4081 tgtaacaaga ggcgtatttg cacacaataa ccaccgtgaa taggtctaaa gtaaaacata
     4141 agaaatattt ggggtttagt ctgcttttta atccatatta ctggattttt gttaagccgt
     4201 ttaacggcgt tccaggggca ggaaaaaagg atattcagga gaaaatgtgc gctatatccg
     4261 acagttatgc tgtgtaagct tactctgctt aagcggatct gccgtcgccg cgaacgtccg
     4321 tctacaggtc gaggggttat cgggacagct ggaaaagaac gttcgtgcgc agctttctac
     4381 gattgaaagt gatgaagtga cgccagaccg tcgctttcgc gcacgcgtcg atgatgccat
     4441 ccgtgaaggt ctgaaagcgc tgggttatta ccagccgacc attgaatttg atctccgtcc
     4501 accgccaaag aaagggcggc aggtattgat cgccaaagtc acgccaggtg tgccggtgtt
     4561 aattggcggc accgatgtgg tattgcgcgg cggcgcgcgg accgataaag actatttgaa
     4621 attgctcgat actcgcccgg ctattggtac ggtactgaac cagggcgatt atgaaaattt
     4681 caaaaagtcc ttaaccagta ttgcgttgcg taaaggttat ttcgatagcg aatttaccaa
     4741 agcgcagctg ggcattgcgc tcggcctgca taaagccttc tgggatattg attataacag
     4801 tggcgaacgt taccgctttg ggcatgtgac ctttgaagga tcacaaatcc gcgatgaata
     4861 cctgcaaaat ctggtgccgt ttaaagaggg cgatgagtat gaatcgaaag atttggcgga
     4921 actgaaccgc cgactttctg ctaccggctg gtttaactcg gtggtggt
//
LOCUS       DADMGC010000209         4817 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.209, whole genome shotgun sequence.
ACCESSION   DADMGC010000209 DADMGC010000000
VERSION     DADMGC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.209"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..1103)
                     /locus_tag="JNZ73_003979"
     CDS             complement(<1..1103)
                     /locus_tag="JNZ73_003979"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY8734064.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICT"
     gene            complement(1314..2711)
                     /gene="ccp"
                     /locus_tag="JNZ73_003980"
     CDS             complement(1314..2711)
                     /gene="ccp"
                     /locus_tag="JNZ73_003980"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HAY8734065.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLVLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            3116..4765
                     /gene="treF"
                     /locus_tag="JNZ73_003981"
     CDS             3116..4765
                     /gene="treF"
                     /locus_tag="JNZ73_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HAY8734066.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDLYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVSREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1220 a         1239 c         1156 g         1202 t
ORIGIN      
        1 gtacagatga actcatacgg cccctgtttg gcgatttcat ccgccagata agcggcaacc
       61 tggtaagagg cgttctgtac tttggtatag ccttcacgac cgaggcgcag gaattcatag
      121 tactgtgcaa ttacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatttga
      181 ccacccaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag
      241 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      301 ttcacacgcg gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccaggaag
      361 ccaccgctgg cagcgtcgat gtgcatgtcg atgtcgatac cggtgtcggc ctggaattta
      421 tccagcgcat cgtgcagcgg ttgtgggaac tcatagttac cggtgtaggt cacgccgaaa
      481 gtcggcacca cgccgatggt gttttcgtca caggcttcaa tcatgcgttt cgggtccata
      541 aacaactgac cggggcgcat agggatctca cgcagctcca catcccagta gcgggcgaat
      601 ttatgccagc agatttgtac cggaccgcac actaggtttg gtttatccgt tggtttgcct
      661 gcagcttcca tacgcttgcg ccaacgccat ttcatcgcca tcccgccgag catacaggcc
      721 tcggaagaac caatggtgtt ggtgccaacg gcctgaccat ttttcggcgc aggcgcatgc
      781 cacagatcgg caaccatatt tacgcaacgc aggtcgatgg ctgcggattg cggatattct
      841 tctttgtcga tccagttttt attgatcgac aaatccatca atttatggac gttttcgtcg
      901 tcccaggtct ggcagaaagt ggctaggttc tgacgagcgt tgccatcaag atataattca
      961 tcattgataa tctggaatgc gacatcatcg cgcatttcgt gcagcggaaa tcgttttgac
     1021 tccgcgatag tagaaatggc ctttgcgcca aaacgtgaat cgagtagttc tgagcggaaa
     1081 tccgttaaca gcttctggtc catttcgaac tccttaaatt tatttgaagg caataaaaaa
     1141 gtaggattta tccgcaatgg aagcaaggca ttacaggctt aatttaaata acaaaatcct
     1201 aagcagaaaa acgacttttt ataatataaa catttaacat gataatatta aaaacagact
     1261 ttattatcgt aataaaaatc gcccaaacat tcctgttcgg gcgatcaaat taattattgt
     1321 ttatcctgca tatacggcgt gtacaccccg ttcagactgt gcaggaaagc tacgatatca
     1381 tccacatcct cctgcggcag ctctttgcct acctgatagc gcagcatcag tttcaccgcc
     1441 ccgtccagcg tcggcacgtc accgcgatgg aagtacggtg ccgttaaagc aacgttacgt
     1501 aaaccgggta ctttctgacg caatttatca cgctcttctt tagtcacatt catacgacca
     1561 atatccgccg ccgtaatttc cccaaagtta aagtcttttt tcagcaccaa cggttcaaag
     1621 gaacgtccgc cgagaataat accaccatga caagttgcac atttattatc tttaaataat
     1681 tgatagcctt ttttctgttg cgccgtcagc gcattttcat ctccacgcaa ccatttatca
     1741 aatggggaat ccggcgtaat taatgttttc tcaaattcag caatggcatc agtaatattt
     1801 tcgccactga aaccttgcgg ataaacttcg aggaactgcg ctttaagctg cggatctttt
     1861 tccagcttag caataatttc gtcccaggat ttcgacgcca tttcaatcgg gttcaacggc
     1921 ggtccacctg cctgatcctg caatgttgcc gcacgaccat cccagaactg ctcaacgtta
     1981 aatactgagt taaataccgt cggcgcatta atcggcccaa ctgcgccacc aacaccaatc
     2041 gatgtttttc tgccatcgac gccccccgca ttcaacgtat ggcaatgagc gcatgaaatg
     2101 gtgctatcag ccgataaacg gggatcgtga tacagcgcaa agcccaacgc cactttttgc
     2161 gcatcggtag gcagtttttg cgggatgggc tgcaccggtt cattgcgatg ctccggagca
     2221 gtatcattgc tggcgtaata ttccgcgcgc tgttttgcaa tccaggccag tatttccgcc
     2281 cgctcttcat cactcacctt acccgcccag tgtagcgcgg tataacgcgt tggtggcata
     2341 gtttcatact gcatcaccca ttcaatctta ttcagatcgc tttgcgaaac gggtttatca
     2401 gccagcagtg ccgcacgcac ggcctcgagg ttaaaagatt tatatccaag cttaatgtcg
     2461 taatccatca actgtttcgc gccaggaata taataatagg cgggtaattc tgccgaaggc
     2521 gtgtggcaat agtcgcatcc tttttcgcgg agaaagccta aaaccttatt attttcactg
     2581 acagcagatg cctgaacatc ggcctgttta ctgcgtttat tatcgtggta ccacacataa
     2641 ccagataacc ctaaatagca aatcgcgacg ccagccaggc cgatcgcggt aatacgtgag
     2701 accattttca ttattcttcc ctcacggtgg tgagttattg tgacccagaa aattttccag
     2761 aatgctgcaa acgtctctgc gagacaaagc catcataaaa aacagggaga tacgcttttt
     2821 gataggaatc aatcaattac atgaatataa actatcaata agatagcctg tgcctattac
     2881 ccagatcagg aatggcatat catgattgcg gcgagagcag gattgaatgt tctgaaaatg
     2941 aaaaacaaca ccaaatccgt atttttataa attccattga tattagtgcg ttatattttt
     3001 tgacaagtta ataaacgtga tccaccgcac gctttgtcgc ccaccaggcg gagcgaatga
     3061 ctacccttaa agaaaagccc gataattagc gacgaatttc ggaggttgga tccttatgct
     3121 caatcagaaa attcaaaacc ctaatccaga cgaactgatg atcgaggtcg atctctgcta
     3181 tgaactggac ctgtatgaat taaaactgga tgagatgatc gaggcagaac cggaacccga
     3241 gatgattgaa gggctgcccg cctctgatgc gctgacgcct gccgatcgct atctcgaact
     3301 gttcgagcat gttcagtcgg cgaaaatttt ccccgacagt aaaacctttc ccgactgcgc
     3361 acccaaaatg gacccgctgg atattttaat ccgctaccgt aaagtgcgcc gtcatcgtga
     3421 ttttgacttg cgcaagtttg ttgaaaatca cttctggctg ccggaggtct actccagcga
     3481 gtatgtatcg gacccgcaaa attccctgaa agagcatatc gaccagctgt ggccggtgct
     3541 aacccgcgaa ccacaggatc acattccgtg gtcttctctg ctggcgctac cgcagtcata
     3601 tattgtcccg ggcggccgtt ttagcgaaac ctactattgg gattcctatt tcaccatgct
     3661 gggactggcg gaaagtggtc gggaagattt gctgaaatgc atggccgata acttcgcctg
     3721 gatgatcgaa aactacggtc acatccccaa cggcaaccgc acctattatt tgagccgctc
     3781 gcaaccaccg gtttttgcgc tgatggtgga gttgtttgaa gaagatggtg tacgcggtgc
     3841 gcgccgctat ctcgaccacc ttaaaatgga atatgccttc tggatggacg gtgcagaatc
     3901 gttgatccct aatcaggcct atcgccatgt tgtgcggatg ccggacggat cgctgctcaa
     3961 ccgttactgg gacgatcgcg acacgccgcg tgacgaatcc tggcttgagg acgttgaaac
     4021 cgcgaaacat tctggtcgcc cgcccaacga ggtgtaccgc gatttacgcg cgggagcggc
     4081 ctccggttgg gattactctt cccgttggct gcgtgatact ggtcgtctgg cgagcattcg
     4141 taccacccag ttcatcccca tcgatctgaa tgccttcctg tttaaactgg agagcgccat
     4201 cgccaacatc tcggcgctga aaggcgagaa agagacagaa gcgctgttcc gccagaaggc
     4261 cagtgcccgt cgcgatgcgg taaaccgtta cctctgggat gatgaaaacg gcatctaccg
     4321 cgattacgac tggcgacgcg aacaactggc gctgttttcc gctgccgcca ttgtgccgct
     4381 ctatgtcggc atggcgaacc atgaacaggc cgatcgtctg gcaaacgccg tacgcagccg
     4441 gttactgaca cctggcggga ttttggcaag cgagtacgaa accggtgaac agtgggataa
     4501 acccaacggc tgggcaccgt tacaatggat ggcaattcag ggatttaaaa tgtatggcga
     4561 tgaccttctg ggtgatgaaa tcgcgcgcag ttggctgaaa acggtgaatc agttctatct
     4621 ggaacagcac aaaatgatcg agaaatacca tattgccgat ggtgtttccc gcgaaggcgg
     4681 cggtggcgag tatccgttgc aggatgggtt tggctggact aacggtgtgg tacgccgttt
     4741 aattggtttg tacggcgaac cataatattt ttacagccag ccgctaactt cctgctggct
     4801 gtaaaattat cctcttc
//
LOCUS       DADMGC010000210         4626 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.210, whole genome shotgun sequence.
ACCESSION   DADMGC010000210 DADMGC010000000
VERSION     DADMGC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.210"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..619)
                     /locus_tag="JNZ73_003982"
     CDS             complement(<1..619)
                     /locus_tag="JNZ73_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAY8734067.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERA"
     gene            complement(734..809)
                     /locus_tag="JNZ73_003983"
     tRNA            complement(734..809)
                     /locus_tag="JNZ73_003983"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(774..776),aa:Thr,seq:ggt)
     gene            complement(816..890)
                     /locus_tag="JNZ73_003984"
     tRNA            complement(816..890)
                     /locus_tag="JNZ73_003984"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(855..857),aa:Gly,seq:tcc)
     gene            complement(1007..1091)
                     /locus_tag="JNZ73_003985"
     tRNA            complement(1007..1091)
                     /locus_tag="JNZ73_003985"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1055..1057),aa:Tyr,seq:gta)
     gene            complement(1100..1175)
                     /locus_tag="JNZ73_003986"
     tRNA            complement(1100..1175)
                     /locus_tag="JNZ73_003986"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1140..1142),aa:Thr,seq:tgt)
     gene            1537..2487
                     /gene="coaA"
                     /locus_tag="JNZ73_003987"
                     /gene_synonym="panK"
     CDS             1537..2487
                     /gene="coaA"
                     /locus_tag="JNZ73_003987"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAY8734068.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2516..3481)
                     /gene="birA"
                     /locus_tag="JNZ73_003988"
     CDS             complement(2516..3481)
                     /gene="birA"
                     /locus_tag="JNZ73_003988"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HAY8734069.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGSGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3478..4506)
                     /gene="murB"
                     /locus_tag="JNZ73_003989"
     CDS             complement(3478..4506)
                     /gene="murB"
                     /locus_tag="JNZ73_003989"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAY8734070.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1180 a         1169 c         1048 g         1229 t
ORIGIN      
        1 tcgcacgctc tggttccgga atgtaagaat ccaggaagcc agccagttcc aggattttcg
       61 cttcccactc tgcgtcgcct tccagcgctt tcagagcaga accacgaacg atcggagtgt
      121 cgtcgcccgg gaagtcgtac tgagacagaa gttcacgaac ttccatttca accagttcca
      181 gcagctcttc gtcatcaacc atgtcgcatt tgttcaggaa cacgatgatg tacggaacgc
      241 ctacctgacg ccccagcagg atgtgctcac gagtctgcgg catcgggccg tcagtcgcag
      301 caactaccag gatcgcgccg tccatctgag cagcaccggt gatcatgttt ttaacatagt
      361 cggcgtgccc cgggcagtct acgtgtgcgt agtgacgggt cggggtgtcg tattcaacgt
      421 gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg atctggtcga
      481 atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca gcggtcagcg
      541 ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt tttgtacgtt
      601 caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg tgatatcacc
      661 acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga aaaaacaggg
      721 aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc cttaccaagg
      781 gtacgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg gaatcgaacc
      841 cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc atcctagaac
      901 tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt attcgagccg
      961 gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt ggtgggggaa
     1021 ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt tggccgctcg
     1081 ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac ctactgatta
     1141 caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca ttctatggag
     1201 acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca cattttatgc
     1261 tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca acatcattgt
     1321 gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat atctgtcaca
     1381 ttgcttttca acgatagctt cctggcagag attttttctt attattcctc cccatctggt
     1441 gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg caaatatcgc
     1501 tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga gcaaacgtta
     1561 atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga ttccgtacct
     1621 atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga tctctcgtta
     1681 gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta tataagctcg
     1741 aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca acgcattcct
     1801 tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc ccgtgtattg
     1861 caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac tacagatggc
     1921 ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa aggcttcccg
     1981 gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc cggcgtgcca
     2041 aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga tggagataaa
     2101 actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca gagcgggatg
     2161 gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt ttcgatatat
     2221 gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct gaaattccgc
     2281 gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt aactaaagaa
     2341 gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa cttaaagcaa
     2401 aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc taatcatgtg
     2461 gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc ctttcttatt
     2521 tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt ccatcctgct
     2581 caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt tctttatcac
     2641 caatgataag tttcactggg cgattaataa aattatccag cttttcccag cgcgacagat
     2701 aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca cgtattagca
     2761 tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg atccacccct
     2821 gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggat ccaatgacta
     2881 tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc agcttgcgat
     2941 cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc agctttcgta
     3001 atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc gccgggcctt
     3061 gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa aaccatttcc
     3121 gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg cccgatttaa
     3181 gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc agcacggcta
     3241 cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg atgggctcag
     3301 gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca cgcagtgtct
     3361 gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac tgctcgccag
     3421 agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg ttatccttca
     3481 tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga cttcaggctc
     3541 aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca gttgcaccac
     3601 atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac ggtgcaccgc
     3661 agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac ctgctgccag
     3721 ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg ccagtaatgc
     3781 tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat tgccattcac
     3841 ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata cttgctgtgg
     3901 cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag gttgccactc
     3961 ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt catgtttaaa
     4021 aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt gcttgcctgt
     4081 cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc cataagcacc
     4141 aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat tttccagacc
     4201 aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc cggctcctac
     4261 atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc ggttgatgat
     4321 caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca gaataagaac
     4381 gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt cgtcttcggc
     4441 acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt ttaaggagtg
     4501 gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg tggggggatg
     4561 gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggccat cctgacggat
     4621 agcctt
//
LOCUS       DADMGC010000211         4597 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.211, whole genome shotgun sequence.
ACCESSION   DADMGC010000211 DADMGC010000000
VERSION     DADMGC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.211"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..213
                     /locus_tag="JNZ73_003990"
     CDS             <1..213
                     /locus_tag="JNZ73_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY8734071.1"
                     /translation="PQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKVMGGGEEL
                     SNLVLLHPNCHRQLHSCEAGSLKKGL"
     gene            356..577
                     /locus_tag="JNZ73_003991"
                     /pseudo
     CDS             356..577
                     /locus_tag="JNZ73_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(598..660)
                     /locus_tag="JNZ73_003992"
                     /pseudo
     CDS             complement(598..660)
                     /locus_tag="JNZ73_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1110..2522
                     /gene="ospC1"
                     /locus_tag="JNZ73_003993"
     CDS             1110..2522
                     /gene="ospC1"
                     /locus_tag="JNZ73_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HAY8734072.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2626..2775)
                     /locus_tag="JNZ73_003994"
                     /pseudo
     CDS             complement(2626..2775)
                     /locus_tag="JNZ73_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2851..4548
                     /gene="ospD3"
                     /locus_tag="JNZ73_003995"
     CDS             2851..4548
                     /gene="ospD3"
                     /locus_tag="JNZ73_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HAY8734073.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGFSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1609 a          819 c          855 g         1314 t
ORIGIN      
        1 ccgcagccgt caggctgcct actcgagtca caaggctctt tgtgtccgat atgccggcag
       61 cgcattaccc atgaaactgg ctggaacgta caccataagg tgaagaaagt tatggggggc
      121 ggtgaagaat tatctaacct tgtcttgtta catccaaatt gccatcgaca gttacacagt
      181 tgtgaagccg gctctctcaa aaagggctta taaaggcttg agcggtgtac cgggaaaccg
      241 gtatgcaccg ttcttagggg agggcagtcc agcaatgaac tgtccttacc cgacaaacat
      301 ccaaaacgaa cggaacgtgc tggaatccgc atataacccg ttgtaaagtg aggaaatgga
      361 cacctcactt gctcatgaga acgcccgcct gcgggcactg ttgcagacgc aacaggacac
      421 tatccaccag atggctgaat acaaccgcct gctctcacag cggatggcgg cttatgcttc
      481 cgaaatcaac cgactgaagg cgctggttgc gaaactgcag cgtatgcagt tcggtaaaag
      541 ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca ttttcacggg tgatctacgc
      601 cacacaggct ctgggttgca gtgactgctc cattaaggcg attttgaact cataagggaa
      661 gtaacaaaaa aactgcaaaa gtacaataat tatatatggc caaactaacc tctattaaaa
      721 aacatcagtt tttcacactt agttgagctt catgaaaaaa tcatcgagga tttcattgca
      781 gaatacacat tccattaact ggaagcactc cataagagga atcagagaaa gtatcgtttt
      841 tttatagtaa aattcattgc tgttcaataa aattgatata gttattaaat tatgaaaata
      901 atttcacaaa aaagattaac ttgagttctt aagcatcgga ttttagatat tttgaaatat
      961 ctggcatcac ataacatcaa aagtatataa tacccttatc taattacatg cagtatattg
     1021 tatatatact caaaaattgt aattcatacc ctgctatata ttgtagggaa agatattaat
     1081 taaaactgtt ttcatataag gttcatttta tgaatatatc agaaacactg aactcagcaa
     1141 atacccaatg caatatagat tctatggata acagattaca tacattgttt ccaaaagtga
     1201 catcagtgcg aaacgctgca caacaaacta tgccagatga aaaaaattta aaagatagtg
     1261 caaatattat taaagatttc tttaggaaaa ctatagcagc acagagttat agtagaatgt
     1321 tctctcaagg ctctaacttt aaatctttaa atatagcaat tgatgcacca tcagacgcta
     1381 aagcctcatt taaggctatt gagcaccttg acagattatc gaagcattat atatctgaaa
     1441 taagggaaaa acttcatcct ctttctgcag aggaactcaa tttgctttcg ctaattatta
     1501 attctgattt aatcttcaga catcaaagta attctgattt gtctgataaa attttaaaca
     1561 ttaagtcatt caataaaatt cagtctgaag gaatatgcac aaaacgaaac acatacgctg
     1621 atgatataaa aaaaatagct aatcatgact ttgtgttttt tggcgttgaa atctctaacc
     1681 atcagaaaaa acaccccctg aatacaaaac atcacactgt tgattttggt gcaaatgcgt
     1741 atatcattga tcatgactct ccatatggat atatgacatt aaccgatcac tttgataatg
     1801 ctattccacc tgttttttac catgagcacc aatcattttt agataaattt tcagaggtta
     1861 ataaagaagt tagtcgatac gtacatggaa gtaaaggaat tatagatgta ccaatattca
     1921 atactaaaga tatgaagtta gggctcggat tatacctgat tgactttatt agaaaaagtg
     1981 aagaccaaag cttcaaggag ttttgctatg gaaaaaatct tgcccctgtg gatctggata
     2041 gaatcataaa ctttgttttt cagccagagt accatatacc taggatggta agtacagaaa
     2101 acttcaaaaa agttaagatt agagaaatat ccttagagga ggctgttaca gcatctaatt
     2161 acgaagaaat taacaagcag gtcactaaca aaaaaattgc tctccaggct ctttttcttt
     2221 cgattactaa tcaaaaagag gatgtcgcct tatatatatt atctaatttt gagataacta
     2281 gacaagatgt tatttccata aagcatgagt tgtatgatat tgagtatcta cttagcgctc
     2341 ataattcaag ctgtaaagta cttgagtatt ttatcaataa gggattggtt gatgtaaaca
     2401 caaagttcaa aaaaactaat agtggggatt gtatgttgga taacgcaata aaatatgaga
     2461 atgcagaaat gataaaacta ttattgaaat atggtgcaac atctgacaat aaatatattt
     2521 aatcaaaatt gaatatcgtt tagcaaaagg cagtatttgc acaaaatatc gatatattgt
     2581 ttattgtcag tatggctcaa tgtgataata gttggaaagt ttgatggggt tcgccccgtt
     2641 gtagcggtag tcgaactgga aggtcttcag gcactgctta cagcgataga gcagcccccc
     2701 tgaactggaa tggccgttcc ggataccccc ctgagtttca gagtaacggg gacaaaccac
     2761 atcaatcttt gccatcaatc atccaaaggg caaagagtac aacaacacta agtctgcgtc
     2821 acaacccatc aatgaaagga atatatacat atgccatcag taaatttaat cccatcaagg
     2881 aaaatatgtt tgcaaaatat gataaataaa gacaacgtct ctgttgagac aatccagtct
     2941 ctattgcact caaaacaatt gccatatttt tctgacaaga ggagtttttt attaaatcta
     3001 aattgccaag ttaccgatca ctctggaaga cttattgtct gtcgacattt agcttcctac
     3061 tggatagcac agtttaacaa aagtagtggt cacgtggatt atcatcactt tgcttttccg
     3121 gatgaaatta aaaattatgt ttcagtgagt gaagaagaaa aggctattaa tgtgcctgct
     3181 attatttatt ttgttgaaaa cggttcatgg ggagatatta ttttttatat tttcaatgaa
     3241 atgatttttc attccgaaaa aagcagagca ctagaaataa gtacatcaaa tcacaatatg
     3301 gcattaggct tgaagattaa agaaactaaa aatggggggg attttgtcat tcagctttat
     3361 gatcccaacc atacagcaac tcatttacga gcagagttta acaaatttaa cttagctaaa
     3421 ataaaaaaac tgactgtaga taattttctt gatgaaaaac atcagaaatg ttatggtctt
     3481 atatccgacg gtatgtctat atttgtggac agacatactc caacaagcat gtcctccata
     3541 atcagatggc ctaataattt acttcacccc aaagttattt atcacgcgat gcgtatggga
     3601 ttgactgagc taatccaaaa agtaacaaga gtcgtacaac tatctgacct ttcagacaat
     3661 acgttagaat tacttttggc agccaaaaat gacgatggtt tttcaggatt gcttttagct
     3721 ttacaaaatg ggcattcaga tacaatctta gcatacggag aactcttgga aacttctgga
     3781 cttaaccttg ataaaacggt agaactacta actgcggaag gaatgggagg acgaatatcg
     3841 ggtttatccc aagcacttca aaatgggcat gcagaaacta tcaaaacata cggaaggctt
     3901 ctcaagaaga gagcaataaa tatcgaatac aataagctga aaaatttgct gaccgcttat
     3961 tattatgatg aagtacacag acagataccc ggactaatgt ttgctcttca aaatggacat
     4021 gcagatgcta tacgcgcata cggtgagctc attcttagcc cccctctcct caactcagag
     4081 gatattgtaa atttgctggc ctcaaggaga tatgacaatg ttcccggact tctgttagca
     4141 ttgaataatg gacaggctga tgcaatctta gcttatggtg atatcttgaa tgaggcaaaa
     4201 cttaacttgg ataaaaaagc agagctgtta gaagcgaaag attctaatgg tttatctgga
     4261 ttgtttgtag ccttgcataa tggatgtgta gaaacaatta ttgcttatgg gaaaatactt
     4321 cacactgcag accttactcc acatcaggca tcaaaattac tggcagcaga aggcccaaat
     4381 ggggtatctg gattaattat agcttttcaa aataggaatt ttgaggcaat aaaaacttat
     4441 atgggaataa taaaaaatga aaatattaca cctgaagaaa tagcagaaca cttggacaaa
     4501 aaaaatggaa gtgattttct agaaattatg aagaatataa aaagctgaat attatctcgt
     4561 ggtgatacac tgaatttgag gtaaccggct catttaa
//
LOCUS       DADMGC010000212         4577 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.212, whole genome shotgun sequence.
ACCESSION   DADMGC010000212 DADMGC010000000
VERSION     DADMGC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.212"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            437..2089
                     /locus_tag="JNZ73_003996"
     CDS             437..2089
                     /locus_tag="JNZ73_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY8734074.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="JNZ73_003997"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="JNZ73_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2349..2462)
                     /locus_tag="JNZ73_003998"
                     /pseudo
     CDS             complement(2349..2462)
                     /locus_tag="JNZ73_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989794.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..2922)
                     /locus_tag="JNZ73_003999"
     CDS             complement(2596..2922)
                     /locus_tag="JNZ73_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY8734075.1"
                     /translation="MPGASNAAMCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWI
                     EYYGKFWSRNFSYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLALVRKQYPKLFTHWYL
                     LRASNE"
     gene            complement(3207..3859)
                     /locus_tag="JNZ73_004000"
                     /pseudo
     CDS             complement(3207..3859)
                     /locus_tag="JNZ73_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904490.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
BASE COUNT         1217 a         1108 c         1055 g         1197 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggtacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgg
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg taaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagaaagg agaaactcgt
     3001 agcgacgttt ctccttttaa acgtgtcaat gtaggcgata ttcgtcttcc ctgcgttcag
     3061 gaccaacccg acttccgaga agcgcaccga taacctgttc ttcagccttg ttgcatcact
     3121 cattcgtgaa caatgtacta caagtcgagt cacccgaggg aacctccccc tctggtgctc
     3181 acggaaccgg acgtgaacct ctcagctcat ccggctcttc tcattgaggc ctgtccccat
     3241 agtaacggcc agtgtgcaaa taacctcgtt tcactccgcg caacacgggc cagccagtgg
     3301 ctggcccgtc gtcggtggcc cctaagtcgc ttgtgtttac ggggcagcca taacacgagt
     3361 ttgcgatcga tttgcctcag cgtcagatac agtgctgatt tataaaaagc accgtagtac
     3421 tttatccagc ctttgatttt cgcattgaac atatgcgcca gctcgtacaa ggacttgtca
     3481 ctgcgattct cctcacctcc tgacggattg cttttcctgc tctggcactc atcgccggac
     3541 tgaagttaac gaaggtctta ccccagcgat tcatcgacag acgcggccgg aatgtatagc
     3601 ccagaaagtt gaagctcgtt tccgggtagt ccccccgtcg gtcagcatcc ttacagtaaa
     3661 ctattcgtgt tttttgcgga tgcagctgca gcccgcattg cgcaagccgc tgcctcagtc
     3721 cacttatcag agcgtcagcc tgccactggc tgtgacagtg gcatacaaca tcatcggcgt
     3781 acctttcgaa gggaacgccg ggaaactgac gttgcatcca catatcaaag gcatagtgca
     3841 ggaacagatt tgagagcagg ggggagatta cgccgccctg cggcgttcct cgaccccgtg
     3901 ccagcattgc tccatccggc atttgcaccg gtgccttcag cctgcgctca atgtacatca
     3961 ccacccagcg tgcctgagta tgatgctgca ctgtcttcag tagcaggtca tgatcgatat
     4021 catcaaagaa gcctttaata tcgacttcca ccacccagtc aaacttccag cagcgtttcc
     4081 tcgcctgcgc aatcgcctgg tgtgcggatt tacccggtcg ataaccatac gagtctgcat
     4141 ggaacagggg ttccagtacc ggctcgagta tctgcttaac gaccatctgg gcgatccggt
     4201 ccgacacggc cggaataccg agcggacgta caccaccgtc agctttggga atgtccacac
     4261 gccttaccgc ctgcggcata taactgccgg aagccattct gttccacagt ttgtagagat
     4321 tgtctgtaac attctcgtca aacccggcca gtgtctgccc gtcaatgcct gccgccccac
     4381 ggttggcttt tactcgttta taagcttccc atacctgtcg tttacttaca ggaaatggct
     4441 ttgccgattt tacagcagtc ctcccatcgg cattgtcgcc cgtggttgcc gccgcaccat
     4501 cagcacaatg gggcaagccc tttgctccgg acccattacg gtccttcacc gctactacgg
     4561 tttactccgt ccctgta
//
LOCUS       DADMGC010000213         4484 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.213, whole genome shotgun sequence.
ACCESSION   DADMGC010000213 DADMGC010000000
VERSION     DADMGC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.213"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(43..234)
                     /locus_tag="JNZ73_004001"
     CDS             complement(43..234)
                     /locus_tag="JNZ73_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734076.1"
                     /translation="MTENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
     gene            complement(934..1791)
                     /gene="repA"
                     /locus_tag="JNZ73_004002"
     CDS             complement(934..1791)
                     /gene="repA"
                     /locus_tag="JNZ73_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HAY8734077.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(1784..1858)
                     /locus_tag="JNZ73_004003"
     CDS             complement(1784..1858)
                     /locus_tag="JNZ73_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HAY8734078.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            complement(2082..2342)
                     /locus_tag="JNZ73_004004"
     CDS             complement(2082..2342)
                     /locus_tag="JNZ73_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HAY8734079.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            complement(2582..3172)
                     /locus_tag="JNZ73_004005"
     CDS             complement(2582..3172)
                     /locus_tag="JNZ73_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAY8734080.1"
                     /translation="MKLIIFILIVLIIATLLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            complement(3211..3420)
                     /gene="hha"
                     /locus_tag="JNZ73_004006"
     CDS             complement(3211..3420)
                     /gene="hha"
                     /locus_tag="JNZ73_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY8734081.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(3466..3729)
                     /locus_tag="JNZ73_004007"
                     /pseudo
     CDS             complement(3466..3729)
                     /locus_tag="JNZ73_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978818.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            3755..4057
                     /locus_tag="JNZ73_004008"
     CDS             3755..4057
                     /locus_tag="JNZ73_004008"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8734082.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTLAELESEILQLRKALNEARLERDILKKATAYFAQESLKNTR"
     gene            4093..>4484
                     /locus_tag="JNZ73_004009"
     CDS             4093..>4484
                     /locus_tag="JNZ73_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8734083.1"
                     /translation="MCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRET
                     YGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQ
                     TFAPTAPNQVWVADLTYVATQEGWLYLAG"
BASE COUNT         1096 a         1120 c         1107 g         1161 t
ORIGIN      
        1 tcaagtctta acgatatccc tgaaacgacc acttaatatt actcacactt ttgcaaatgc
       61 attaaatgct gcttgtatta ttctgatgcc gcgccagcgg catggaaggc ggcacgctgt
      121 ggttacatgt gataccggag taaaaccgcc gaagcccggc gtcagccggt actgattgac
      181 agatttcacc ttacccatcc ccagccctgc taagccatac ccgttttcag tcatgataga
      241 gcttctgtgc gcggtcggag tggtcccgac gagggtttta cccgaagtcg gggcgtatct
      301 ccgcgttagc gggccgtgag ggccgcttac gagcgtgtac tcgatacgtc cagccagaag
      361 actgacagcg atgatgatgt agttacaaca ttcataatta aaagcgactc tgttcccgcc
      421 cttcgggcgg ggcggcggcg cttttcagtt atgaggaggg gcgttgtggt ttcagttctg
      481 cgttggactt gggtttttta ggggtttatg tttgcatgtt gtaactaaac cggcttcggt
      541 tggggcccgc cgcttgcggt gggaggtgca tatctgtctg tccacaggac aggcagtgaa
      601 caggttttct ttttaaatga atgtaattaa gtagtttaaa ggagatatac acaggtgttt
      661 aaaagataca ttgcaccctg tagggttgac ggctggcgct ttatgacgtg aacggttgta
      721 accttacggt gaagtccctt gcagtttaat gtggataagc aaaattcccc gtctgtgagg
      781 cgtgttttgt atcaaaaaca ggggggaccg gatgcaccag aaggtggatg atgaggttgt
      841 tttttgtatg tagcgctgat tttttgtgca ctggcgggct tcaggcgtgc gaatgcctcc
      901 ggcgcgtgcc ggattattct gaggaggtca ctttcagggg gaagctgtgg ccagccggct
      961 gtaattacgg ttacgtgaca gaatcatgcg ctccttcaca cgacgctcca cttcacgttt
     1021 taccgcctca cgactggcag tgaagcgccc ttccgagatt tcacgcgtca gttgccgttt
     1081 caccagggtg acaatatcct gacgctgcct gtccgcatca cgacgcgcac gggcacgctt
     1141 cattccacgg gacttaagct ctgtctggta actgcggaaa cgctcacgaa caaaacgcca
     1201 ggctttcgcc atcagttcat ccatacccag ggtatccagc ccctgctttt tgcgctgtct
     1261 gttttcccat tccacacgac tgcggcgcgc ggcggccact gcctcctctg atacatcgag
     1321 ggcagcaaac agtgcagatg tgaacgtgat atcggtcgga atgtagcacc cgataagcgg
     1381 gtcatattcc gtctggtagg taatcagtcc cagctctgca aggaacgtca gggcccgggt
     1441 ggcccgggtg atggagagtt ttccggcagc agactccgtc gccagtccgc actcaatggc
     1501 cagcgtggtg atggaacact ggacgcggtt ggccagtggg tcatagtgaa aacacagccc
     1561 ctgcagcagc gcatcaatgg cccggcgacg tagtaccggt ggcatgcgcc gacgcagacc
     1621 aagcgaacgg gcatgtgcca catgaatggc gaaatcaaaa cgggaggtga agcccaccgc
     1681 cttttccatc agtttcccgc agaacggaag cgtccgggca ccttcacggg gtgtgaacac
     1741 cggattcggg ttctttacct ggcggtaata agtttgttga agatcagtca caccatcctg
     1801 cacttacaat gcgcagaagg agcgagcaca gaaagaagtc ttgaactttt ccgggcatat
     1861 aactatactc cccgcatagc ttgattgttg gctatacggt ttaagtgggc cccggtaatc
     1921 tttctgactc gccaaagtta aagaagatta tcggggtttt tgcttttctg gctcctgtac
     1981 atccacatca gaaccagttc cctgccacct tatggcgtgg ccagccataa aattccttaa
     2041 acgatcagta atctagcatg ctacgccaca aagtaaagtc tttactttag tatatccagt
     2101 ctctgcagtt catctttgat gattttctca acgaactgag cctgtgttat cccctctctc
     2161 tcgcagtact caaccatgag atctttcaga ggatttttga caaaaacttt tatctctttg
     2221 tgtgtaagac gttttcttgc aacagcggcc atttgtttct cagagtcagt cataggctta
     2281 cctctgcgca caaaccgctt ttgactcaat gaggaagtca ctgcattttc tgtctgcgac
     2341 atctcgcctc ctcaatactc aaacagggat cgtttcgcag aggatactac agttttttga
     2401 aatcagcgac ttgagaattg tggcgaagat ctgtgttggc gcacaaatca acggggatta
     2461 ctgtcgttta atgtgattta aactgtgaaa tagtatggtt ttcagttatg gaaacgccgg
     2521 gagcgaggaa aacttgcttt ttcccgtttc cggggttgga caactgagca acgcgaaggc
     2581 gtcagctacg atgttccggt gactgctgat cagcccctgt tgtattcagc cattctccgg
     2641 tcatctgcag cagttttctg gcatcgtggc ttctgagcaa cggaatacca gcctgcctca
     2701 gaacctcttc cagaagaata tcccggcgtc tgcgttccgg tcgtaaatgg ctggcatcat
     2761 ccagctctac tgctgcaaca atactgaacg aacgcagctc aacgataacc acatcaacat
     2821 gccactgaga caccatcctg aataactgcc accactggcg tgatcgtggc cggatattcc
     2881 cgttcagctg gacgatatcc gcgacccgta cctgcggaca cagataccac cgcttcatat
     2941 ccacaattct gaacagtcct ttcataaaac tgacttccct ttctgtcagc agcgtcttct
     3001 gacgttgcca gtattcaccg ctggacagta ttcgctctgc atcaccagtc cgtatacctg
     3061 ccgcatgcaa ttgcatcagc aaaggagaat gctgatttac acttctgagg ataatccgga
     3121 taagtagcgt tgcaataatc agaacaatga gaataaaaat aatcaacttc attatttatc
     3181 tgctcctggc aattaattat tgctgaatta ttaccttaca tatttccata tctcttttgg
     3241 tatcctgtca tataactttc cggtgattaa ttccgctagc cggtggtctg ctgctgaatt
     3301 aaatgattca cgttcactgt ttgataaact gtctctgttt ttatgaataa ttttttccag
     3361 cgtattgact gatgaacatc gccgtaactg atacagccat tcctgttttg tttttgccat
     3421 ttttattccc gctgagaaca gatgaatgat aggattgcca tcgctttatt tacggtgcct
     3481 ccatatccag gggggaaccg gatcagcatc tgaccagagc ccacgttttt gttgtctggc
     3541 ttcgttctga aggacaggta gcacgggatc tgtgttgtac tgctcatata cccaggcagc
     3601 cccggcccgg accataaact gattaatatt cattccgtct ggtgcataaa cctgcccgag
     3661 aatacgacca taacgatctc gctgaaaata tgaggtagcc tgagtttaac ggacactcct
     3721 tcctgaaata gaatggcatc agaaggagct aataatgagc agaaaaaccc aacgttactc
     3781 taaagagttc aaagccgaag ctgtcagaac ggttcttgaa aatcaacttt cgatcagtga
     3841 aggcgcttcc cgattatccc ttcctgaagg cactttagga caatgggtta ccgccgccag
     3901 aaaagggctc ggtactcctg gttcccgcac gctggctgaa ctggaatctg aaattctgca
     3961 actgcgtaag gcgttaaatg aagctcgcct tgagcgagat atattaaaaa aagcaacagc
     4021 gtattttgca caggagtcgc tgaaaaatac gcgttaatcg aacaatggcg acaacaattt
     4081 cccattgaag cgatgtgtca ggtatttggt gtatccagga gcggttatta caactgggta
     4141 cagcatgaac cctcagacag aaaacaaagt gatgagcggc taaaactgga gattaaggtg
     4201 gcacatatcc gcactcgcga aacatatgga acccggcggc tccagacgga gctggcagag
     4261 aatggcatca tcgttggtcg tgaccgactg gcacgtcttc gtaaggagct gaggctacgc
     4321 tgtaagcaga aacgcaagtt cagagcgact acgaacccga accacaatct gccagttgcg
     4381 ccaaatctgc tgaaccagac gttcgctcct acagcaccaa atcaggtctg ggtggcggac
     4441 ctgacgtatg ttgccacaca ggagggatgg ttgtacctcg ctgg
//
LOCUS       DADMGC010000214         4441 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.214, whole genome shotgun sequence.
ACCESSION   DADMGC010000214 DADMGC010000000
VERSION     DADMGC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.214"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..215)
                     /locus_tag="JNZ73_004010"
     CDS             complement(<1..215)
                     /locus_tag="JNZ73_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HAY8734084.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDA"
     gene            525..2297
                     /gene="aspS"
                     /locus_tag="JNZ73_004011"
     CDS             525..2297
                     /gene="aspS"
                     /locus_tag="JNZ73_004011"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAY8734085.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2415..2867
                     /gene="nudB"
                     /locus_tag="JNZ73_004012"
     CDS             2415..2867
                     /gene="nudB"
                     /locus_tag="JNZ73_004012"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HAY8734086.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2896..3636
                     /locus_tag="JNZ73_004013"
     CDS             2896..3636
                     /locus_tag="JNZ73_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY8734087.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3671..4192
                     /gene="ruvC"
                     /locus_tag="JNZ73_004014"
     CDS             3671..4192
                     /gene="ruvC"
                     /locus_tag="JNZ73_004014"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAY8734088.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4194..>4441)
                     /locus_tag="JNZ73_004015"
     CDS             complement(4194..>4441)
                     /locus_tag="JNZ73_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734089.1"
                     /translation="KFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFLGSI
                     PWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1100 a         1107 c         1233 g         1001 t
ORIGIN      
        1 gcatcaaccg gctgttttaa tgcttcggcg taatcggcag accagccaac tcgcaccaga
       61 aacacgggct gaccgctggc gcgaaatttc gccgccagct tcccggcgcg attaactacc
      121 tcatcggcag tatgtggacc accggcaaaa ggtaagatgc cttcttgtaa atcgatcacc
      181 accaacgcag tggttttagc attaagttca agcatgctga ctcctgaaaa aacaaaatcg
      241 tgcctcacac cttaatgcga ttagccgatg gcggaacgac aaattttgtt aatttttatg
      301 agttcgggca acacctttat agcaaactgg cgtagagtct gatttcaggc ccgatataaa
      361 gtggtaacga ggcgttccag cgcgggccag gctcttacgg tgcggcagaa tttccagtat
      421 aatagccgcc ttttttcatt cagttgtgac atacagctaa cgctgcgact tggtcacctg
      481 cgcagcaggg ggcatccgcc ggcagctaag ttaagggata tctcatgcgt acagaatatt
      541 gtggacagct ccgtttgtcc cacgtggggc agcaggtgac tctgtgtggt tgggtcaacc
      601 gtcgtcgtga tcttggtagc ctgatcttca tcgatatgcg cgaccgcgaa ggtatcgtgc
      661 aggtattttt cgatccggat cgtgcggacg cgttaaagct ggcctctgaa ctgcgtaatg
      721 agttctgcat tcaggttacg ggcaccgtac gtgcgcgtga cgaaaaaaat attaaccgcg
      781 atatggcgac cggcgaaatt gaagtgctgg cgtcctcgct gactatcatc aaccgcgcag
      841 atgttctgcc gcttgactct aaccacgtca acaccgaaga agcgcgtctg aaataccgct
      901 acctcgacct gcgtcgcccg gaaatggcgc agcgcctgaa aacccgcgct aaaatcacca
      961 gcctggtgcg ccgttttatg gatgaccacg gcttcctcga catcgaaact ccgatgctga
     1021 ccaaagccac gccggaaggc gcgcgtgact acctggtgcc ttctcgtgtg cacaaaggta
     1081 aattctacgc actgccgcaa tccccacagt tgttcaaaca gctgctgatg atgtccggtt
     1141 ttgaccgtta ctatcagatc gttaaatgct tccgtgacga agacctgcgc gctgatcgtc
     1201 agcctgaatt tactcagatc gatgtggaaa cttctttcat gaccgcgccg caagtgcgtg
     1261 aagtgatgga agcgctggtg cgtcatttgt ggctggaagt gaagggcgtg gatctgggcg
     1321 atttcccggt aatgaccttt gcggaagcag aacgccgtta tggttctgat aaaccggatc
     1381 tgcgtaaccc gatggaactg accgacgttg ccgatctgct gaaatctgtt gagtttgcag
     1441 tatttgcggg gccggcgaac gatccgaaag gccgtgttgc cgctctacgt gttccgggtg
     1501 gcgcatcgct gacccgtaag cagatcgacg aatacggtaa cttcgttaaa atctacggcg
     1561 cgaaaggtct ggcttacatc aaagttaacg aacgcgcgaa aggtctggaa ggtatcaaca
     1621 gcccggtagc gaagttcctt aatgcagaaa tcatcgaagc catcctggat cgtactgccg
     1681 cgcaagatgg cgatatgatt ttcttcggtg ccgacaacaa gaaaattgtt gccgacggga
     1741 tgggcgcact gctcctgaaa gtgggtaaag accttggtct gaccgacgaa agcaaatggg
     1801 caccgctgtg ggttatcgac ttcccgatgt ttgaagacga cggtgaaggc ggcctgacgg
     1861 caatgcacca tccgttcacc tcaccgaaag acatgactgc tgcagaactg aaagctgcac
     1921 cggaaaatgc ggtggcgaac gcttacgata tggtcatcaa tggttacgaa gtgggcggtg
     1981 gttcagtacg tatccataat ggtgatatgc agcagacggt gtttggtatt ctgggtatca
     2041 acgaagagga acagcgcgag aaattcggct tcctgctcga cgccctgaaa tacggtactc
     2101 cgccgcacgc aggtctggca ttcggtcttg accgtctgac catgctgctg actggcaccg
     2161 acaatatccg tgacgttatc gccttcccga aaaccacggc ggcagcgtgt ctgatgactg
     2221 aagcaccgag ctttgctaac ccgactgcac tggctgagct gagcattcag gttgtgaaga
     2281 aggctgagaa taactgatat gactcaaata cacgaaatca ttcgcgttgc atcaaggcgg
     2341 caactgagtg aactcccatg agcatagata actatgtgaa tgggatgagc gaaggcagtc
     2401 aacgaagagg cagcgtgaag gataaagtgt ataagcgtcc cgtttcgatc ttagtggtca
     2461 tctatgcaca agatacgaaa cgggtgctga tgttgcagcg gcgtgacgat cccgatttct
     2521 ggcagtcggt aaccggcagc gtggaagagg gtgaaaccgc gccgcaagct gccatgcgcg
     2581 aagtaaagga agaggtcacc attgatgttg tcgctgaaca actgacctta attgactgtc
     2641 agcgcacggt agagtttgaa attttttcac atttacgtca tcgctatgcg ccgggcgtga
     2701 cgcgtaatac ggaatcatgg ttctgtcttg cgcttccgca cgagcggcag atcgttttca
     2761 ctgaacatct ggcttacaag tggcttgatg cgcctgctgc ggcggcgctc actaagtcct
     2821 ggagcaaccg gcaggcgatt gaacagtttg taattaacgc tgcctgaaca ggcagggcta
     2881 tctggagata tttttatggc aggtcatagt aaatgggcca acaccagaca tcgtaaagct
     2941 gcgcaggatg ctaagcgcgg taaaatcttc actaaaatca ttcgtgagct ggtaaccgcg
     3001 gctaagctgg gcggtggcga tccggacgct aacccgcgtc tgcgtgcggc ggtggataaa
     3061 gcactgtcta acaacatgac ccgtgacaca ctgaaccgcg caattgcacg tggtgtgggc
     3121 ggtgatgatg atgcaaacat ggaaaccatc atctacgaag gttacggtcc tggcggcacg
     3181 gcaatcatga ttgaatgtct gtctgacaac cgcaaccgta ccgttgctga agtgcgtcac
     3241 gcattcagca aatgtggcgg taacctcggc actgacggtt ccgtagctta tctgttcagc
     3301 aaaaaaggcg tgatctcctt cgagaaaggc gacgaagaca ccatcatgga agcagctctg
     3361 gaagcaggtg ctgaagacgt tgtgacatat gatgacggcg cgattgatgt ctacactgca
     3421 tgggaagaaa tgggtaaagt gcgcgacgct ctggaagcgg caggtctgaa agcagacagc
     3481 gcggaagttt ccatgatccc gtccaccaaa gctgatatgg atgcagaaac cgcgccgaaa
     3541 ctgatgcgtc tgatcgatat gctggaagat tgcgacgacg tgcaggaagt ttaccataac
     3601 ggtgaaatct ctgatgaggt tgcagcgact ctctgatgat gcctgctaaa cagcaaaacg
     3661 gagacgcgtg atggctatta ttctcggcat tgacccgggt tcgcgcgtga ccggctacgg
     3721 cgtcatccgc caggtaggta ggcaactgtc ttacctgggt agcggatgca tacgcaccaa
     3781 agtggatgat ttaccgtctc gtctgaagct catctatgcg ggcgtgacgg aaatcatcac
     3841 ccagttccag cctgattatt tcgccattga acaagtcttt atggcgaaga acgctgactc
     3901 agccctgaaa ctgggccagg cgcgcggcgt ggcgattgtt gcggcggtga atcaggaatt
     3961 gccggtattt gaatacgcgg cacgtcaggt aaagcaaacg gtggtaggta tgggtagtgc
     4021 cgaaaaaagc caggtgcagc atatggtccg caccttgctg aaactgcccg ctaatccaca
     4081 ggcggatgcc gccgatgcgc tggcgattgc catcacccac tgccacgtta gtcagaatgc
     4141 gatgcagatg agcgaatcgc ggctgaacct ggcgagaggg cgactgcgtt aagttatacc
     4201 gcctcggtca gttctggctg aggcgtttca actccctccg cgtgaatcaa caccaaaccc
     4261 ggatgatgcc acaaactggc tggcgtgacg gttttacgct cccacggaat agaacctaag
     4321 aaccagaatt tccagaaagt gagttttgca ttcggattgc tatttaacag ttgtccaaac
     4381 gtctctattt cacccaccgg aatacatagc gcctctttat aaatatcaaa gacaaatttt
     4441 g
//
LOCUS       DADMGC010000215         4416 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.215, whole genome shotgun sequence.
ACCESSION   DADMGC010000215 DADMGC010000000
VERSION     DADMGC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.215"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..203)
                     /locus_tag="JNZ73_004016"
     CDS             complement(<1..203)
                     /locus_tag="JNZ73_004016"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="FAD-binding subunit; with XdhA and XdhC
                     participates in purine salvage; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HAY8734090.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGS"
     gene            complement(214..2511)
                     /gene="xdhA"
                     /locus_tag="JNZ73_004017"
     CDS             complement(214..2511)
                     /gene="xdhA"
                     /locus_tag="JNZ73_004017"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HAY8734091.1"
                     /translation="MEAREATATGESCMRVDSIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATHTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASTGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAFESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     PLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            2926..3681
                     /locus_tag="JNZ73_004018"
     CDS             2926..3681
                     /locus_tag="JNZ73_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAY8734092.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            3760..3833
                     /locus_tag="JNZ73_004019"
     tRNA            3760..3833
                     /locus_tag="JNZ73_004019"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3792..3794,aa:Gly,seq:ccc)
     gene            3968..>4416
                     /locus_tag="JNZ73_004020"
     CDS             3968..>4416
                     /locus_tag="JNZ73_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY8734093.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATEHLTVDQHRYINDVIGVLKACQDRLKG"
BASE COUNT         1115 a         1101 c         1131 g         1069 t
ORIGIN      
        1 gagccatctt ccgccagcgt aattccccgc agctccgcca gattatgaat atcaacaata
       61 tggcgataac ggtcattgtg atggtggagc tgaatcagta cgtcagtgcc accggcgagc
      121 agtttggcct gcgggttgtc agccagcagg ttgatggcat cggcaagggt tgctgcgcga
      181 tggtaagaag caaaatcaaa catgatgtta tcctcaaatc aatcctgcca gatggaactc
      241 ttcatataac cgttttggcg tcagcggcag tgtattgatt gcaacaccgg tagccatctt
      301 caccgcgtta cgaatagcag cggcaacagg aattatgggt ggctcaccca gtgacttatg
      361 tccgtatgcg gattgcggct cattgatttc gacgaacgcg ctttccagtt gtggcagatc
      421 cggcatggtc ggcattttgt aatccagcag attggggtta cggaccacgc cgcttttagc
      481 atcgatgatc atctcttcaa atagcgccca gccaatgccc attcccattc cgccgtgtac
      541 ctgaccttct gccagcagtg gattaagaat atgccctgaa tcatgaacgt tgaggatgcg
      601 gttgatggtg actttgcaca gcgcaatatc gaccgtcaga tcaacaaagg tacagccaaa
      661 cgccggtggg ttagtggtgg ttttgatgga gctttcagca gagagctgcc cgccgcgttc
      721 agggtggtag aaagcgtcca tcgccaaatc ttttaacgac attaacggct cttccggtcg
      781 ttcaaccagc acgatatggc cttttatcag gggcaaattc atcgctgact gatgtagcat
      841 gactgcggcg tgagcgatga ttttctcttt taataatagt gccgcactgc gcagcgcagg
      901 cgcggcaaca tagctctggc gtgaggcaaa tgcgccggga tcgaacggcg taacgtcggt
      961 atcttgtgtt gaaataacgc gaacgtcgct gaccggaacc cccacggttt ctgccaccat
     1021 ttgcgagaag acggtgtcgg caccctgacc gatttccgtc gcgccgcttt gcacgttgat
     1081 ggttccatcc tgattcatca gaaggcgcgc gcctgctatt tctacgccga caggccaggt
     1141 gttagaggtg tagctaaaac aggcgacgcc aacgccgcgg cgcaaattgc cttgctggtt
     1201 ctggcattct gcacggcgtt tttcccattc aaagattttc cggccttttt caagacactc
     1261 cggcaaccct gcgctgcaaa tacgtttgcc cgtgagcgga ttagcatctc cttcgcgggc
     1321 ggcgttgcgt aaacgaattt caacaggatc aatacctaac gctgtcgcgg cgtcatcaag
     1381 catagactca aaggcaaata cgacttgtgg cgcgccataa ccacgcatcg caccagccga
     1441 ggggaggttg gtatagcagg tctttgaact gtaagcgtag gcacaacgag gataaaggta
     1501 agcgacttta ttcccccccg tagaagcgat ggagtgcccg tgagatgcat aagcgccggt
     1561 gttagacaga acatccagac tataaccttt caaggttccg tcgcggttca cgcccatttg
     1621 cccgtcaata gtaaaagcgt ggcgggtatg ggttgcgagg aaacactctt cacggctaag
     1681 ggaaactttc accggaatgc cgccaagctt gctggtcagg aatgccgcca ttggctcttc
     1741 cagtacatcc tgtttattac caaaaccgcc accgacaaac ggtttgatga ctcgtacgca
     1801 tgaccaggga atatccagcg cctgaccaac cacgcggcga acaatgtgcg ggatctgggt
     1861 gctggaaacg atggtaattc gcgagtcatc ctccatccac gccagcgatg ttacgctttc
     1921 catgtgacaa tgttgaataa cgggagtctg atagtgcccc tgtacctggt agtcggcggc
     1981 atcgattgtt tgttggacat tgcccgtcga catcgtgctt tgtttcagta aattgccgcc
     2041 gttatggatt ggtgcagcgt cttctgtcag cgccgcttct ggcgtggtga taacgggtaa
     2101 ttcttgccac tcaatgctga ccaattgcgc cgctttttct gccgtgagtt catcgcgggc
     2161 cacgacgatg gcaacggcgt caccatgatg acgaacatgg cgagttagca gtgcgcgatc
     2221 ggcggtatcg cgcttgtttt cgtcaagtgt ccaggcatgc ccagctgtag cgaatggaat
     2281 atcaggcaca tcttcccaag taaaaatcgc cagtacgccc ggtaaacttc tggcttgttc
     2341 atcattaata cttacggcat aaccatgtgc gatagggcta cgtacatatt tcgcgtaaca
     2401 catgcccgcc ataacataat cgtcagtata tcgtgcccgc ccggtgacct tagcaatgga
     2461 atcgacgcgc atgcatgatt cacccgtagc ggttgcttcc cgcgcttcca tagaaatccc
     2521 ctcgatttga acatttggtt cttatggcaa agatttagtc taagcaggca agatttacgc
     2581 cagaaacgac gataagcgtt aggggaggaa ggaagtgtga tgacgttaaa aaaacacaga
     2641 tatgatacga aacaggactc tgaataggga cagtgatacg tatcaatatg agtggcgata
     2701 aaatcaatgt gataaatcag gattattacg catcaggatg ggaaataggg taaaaagtca
     2761 ggaacttcga tacctctaca gcgaatattt tgctgatgat acttcccttt gccaaatgaa
     2821 attaacctct ttctctatta ccacgttttt ccagaagcaa gagattgggt atcaagaggc
     2881 tggctgctat gatatggcgt cttgtttttt aagcgaggaa agattttgag tgcgggacgc
     2941 ctgaataaaa aatctctggg tatcgtgatg tttttatcgg ttggactgct attggcgggc
     3001 tgttcgggta gcaaatcatc cgatacagga acgtattccg gctccgttta caccgtgaaa
     3061 cggggggata cgctatatcg tatttcgcgc accacgggaa ccagcgtaaa agagctggcg
     3121 cgactgaacg gcatttcccc cccttacacc attgaagttg gtcagaaact aaaactgggt
     3181 ggggcgaaaa gtagcagtag tacacgtaaa tcaaccgcca aatcaacgac caaaaccgca
     3241 tcggttacac cgtcatcagc ggtaccgaaa tcttcctggc cgccagtagg gcaacgttgt
     3301 tggttatggc caacgacagg gaaagttatc atgccgtatt cgacagcaga tggcggcaat
     3361 aaagggattg atatctcagc tccacggggt acacctattt acgccgcggg tgcaggaaag
     3421 gtggtgtatg tgggcaacca gctgcgtggc tacggtaatc tcatcatgat taaacacagt
     3481 gaagattaca ttacggctta cgcccataat gacacgatgc tggtaaataa tgggcaaagc
     3541 gtgaaggctg ggcaaaaaat cgccactatg gggagcacgg atgcggcatc tgttcgcctg
     3601 catttccaga ttcgttaccg tgcaacggca attgatccgc tacgttactt gccgccgcag
     3661 ggcagcaagc caaaatgctg atggcgaatt aatcagcagt cagcagcgcg gcacttgcta
     3721 aggagagcgt aaggtttata atgccttacg catctcgaag cgggcgtagt ttaatggtag
     3781 aacgagagct tcccaagctc tatacgaggg ttcgattccc ttcgcccgct ccatgacact
     3841 tcttaaagtt caataactta ctgaatcata agggatatgg cttaacgcct tccgatagtt
     3901 ttactcactg taaccaacac tgtgactaac tttgttgaag tagtgagcag acctaaggtc
     3961 tcagtacatg ctcaactcaa gaacctacct ttatcagcgt aatggtgtct tttacatccg
     4021 tcttaggatg aagaccactg gtcgccttac cgcttcgctt ccttctcaca accgttataa
     4081 actagcgtca gtatctttac ggaccaagga tagacgtacc gctatggctc actcccggca
     4141 catcaagtca gcacttaaag caatccatgc agacaaccct aacgcctctt atgaggagct
     4201 acgggagcac ctgaagacca ttgtcgagtg ggaactcagt gtcagccgcg atgacctgaa
     4261 cgaccctgag tcctatcagc tttacgttga ccagtacgac gatattaagt ccaaccttcg
     4321 ggaagctgta gcgactgagc acctgactgt agaccagcat cgctatatca atgacgttat
     4381 cggtgtgctt aaagcctgtc aggatagact caaagg
//
LOCUS       DADMGC010000216         4378 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.216, whole genome shotgun sequence.
ACCESSION   DADMGC010000216 DADMGC010000000
VERSION     DADMGC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.216"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(64..822)
                     /locus_tag="JNZ73_004021"
     CDS             complement(64..822)
                     /locus_tag="JNZ73_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAY8734094.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="JNZ73_004022"
     CDS             complement(830..1933)
                     /locus_tag="JNZ73_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY8734095.1"
                     /translation="MTKVFLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLILASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..3113)
                     /locus_tag="JNZ73_004023"
                     /pseudo
     CDS             complement(1943..3113)
                     /locus_tag="JNZ73_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3181..4206)
                     /locus_tag="JNZ73_004024"
     CDS             complement(3181..4206)
                     /locus_tag="JNZ73_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8734096.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1214 a         1147 c         1023 g          994 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 gaattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagcccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagga
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa
     2581 atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat
     2641 caaacgcgct cacagcttgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga
     2701 agaagatttg caatattacg gaagatctca atataaatcg tggaaagctt tcgtagcagc
     2761 cagttatccg aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata
     2821 cacaatgcag aaaccagtag cgtattgagt aagccgacaa taaaaacgcg tccgtaggtg
     2881 tcgccctgct ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga
     2941 aaggcaaaac ctgaagtaat gccacgatta ttgagattcg ttacagtgtt ttgaaataac
     3001 caaccaacaa tgccgacaac agcaacaacg gcaaggatct gaaataacca ggcacgaacc
     3061 gtagggttgg caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc
     3121 tcggaaagca taacgctgaa gagagcgccg ccgtagcgac gctcgttacc tcacaccgac
     3181 ttaacgcacg ggcggcgcgt actgaatacc gccgttattc cagagattat tttgcccacg
     3241 tttaattttc agcgggcttt ctgaacctac gttacgctca aaaatttccg agtagttacc
     3301 cacctgtttg atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc
     3361 gccttcttta cccaacagat gtgccatatc aggcgttgct ggattagccg ctttttcatc
     3421 gacgttctgg gaattgatgc ccatctcttc agcatttagc atggcgaaaa gcgtccagcg
     3481 tacaatcgag aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga
     3541 gataacctcc ggtaagacaa tccattcagc cgggttgctt aatttgatgc gcaaggcata
     3601 cagttgcgac tgatccgaag ccagcgtatc gcagcgacca gattccagtg ccttcgctga
     3661 ttcgtcagag cgatcgaaag tcactggtgt gtacttcata ttgtttgcct tgaaatagtc
     3721 ggcgacgttg agttcggtat cagtacccgc ctgaatacag acggtagcgc catccagttc
     3781 tttcgcgctt tttagccccg ctttatcgtg cgtcaggaag ccaatgccgt cgtaataagt
     3841 gacgccggta aatgccattc ccatcccggc atcgcgagat gaagtccagg tcgtattacg
     3901 ggagagcaaa tccacttccc ctgactgtaa agcggtgaag cgttcttttg ctgtgagtgg
     3961 ggtatatttc actttcgtgt cgtcaccaaa tacagcagcg gcaacaccac gacaaacatc
     4021 aacatcaata cctgaaaact taccgtctgc atcggcataa gagaacctag gtaatccatc
     4081 actgatcccg cattgcacaa aacctttttt ctgaacggca tcaagcgtcg cgccagcatg
     4141 cgcctgattt gcaacggcaa gcagcacgct ggcggcagcc agtgtggcta tcatcatctt
     4201 tttcataatg cttcctgtga ggcgaaaatt atcgttatag gacgtttggg aaacgtcttt
     4261 tacctgtctg tggccatcct cttataagca aaagtagtgc cagcttttgc gcacgatttc
     4321 gcgtcgcaat aagctgagtg tagacaggaa ataataaaaa cagcgccccg caatggtg
//
LOCUS       DADMGC010000217         4340 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.217, whole genome shotgun sequence.
ACCESSION   DADMGC010000217 DADMGC010000000
VERSION     DADMGC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.217"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..324
                     /locus_tag="JNZ73_004025"
     CDS             <1..324
                     /locus_tag="JNZ73_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HAY8734097.1"
                     /translation="DDALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIP
                     CPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIP
                     SQADF"
     gene            383..586
                     /locus_tag="JNZ73_004026"
     CDS             383..586
                     /locus_tag="JNZ73_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HAY8734098.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            586..1611
                     /locus_tag="JNZ73_004027"
     CDS             586..1611
                     /locus_tag="JNZ73_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8734099.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(1664..1753)
                     /locus_tag="JNZ73_004028"
     tRNA            complement(1664..1753)
                     /locus_tag="JNZ73_004028"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1717..1719),aa:Ser,seq:cga)
     gene            1847..2644
                     /gene="mtfA"
                     /locus_tag="JNZ73_004029"
     CDS             1847..2644
                     /gene="mtfA"
                     /locus_tag="JNZ73_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HAY8734100.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2745..2820
                     /locus_tag="JNZ73_004030"
     tRNA            2745..2820
                     /locus_tag="JNZ73_004030"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2778..2780,aa:Asn,seq:gtt)
     gene            2982..4244
                     /locus_tag="JNZ73_004031"
     CDS             2982..4244
                     /locus_tag="JNZ73_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8734101.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHLELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
BASE COUNT         1212 a          999 c         1036 g         1093 t
ORIGIN      
        1 gatgatgcac tgttacaatt gcgggaattt atcgacgaaa actccggtga attttttgtt
       61 caggtctggg gaaatggagc caacttcgac aacacgattt tgcgccgttc atacgaacgg
      121 caggggatcc cctgcccgtg gcgttactac aacgatcgcg atgtacgcac aatcgttgag
      181 ctggggaaag ccatagactt cgatgccaga acggctattc cattcgaagg tgagcgccat
      241 aatgcacttg atgacgctcg ttaccaggca aaatacgttt cagctatctg gcaaaaactg
      301 atcccgagtc aggctgattt ttaatgttca accctaattg ccgctaaccg tatatagtta
      361 gcggcggtta tgagatatag ctatgagcag cttattttta accgaagatg aattgctaat
      421 attaacgggc tgcaaatatg caagccacca gcgaaaatgg ttaatggaaa acgggcttcc
      481 gttctatacc aatcgtagtg gcaaaccgat tgtcagccgg gatctattta cctgcaataa
      541 aactttacca ccacgcgagg tagagccgaa ttttggtgcg atctgatggg aagacgaagg
      601 aaaaatcctg aacacgaaaa attacctcca aatgtatacc caaataaata tagttatgta
      661 tggaaaccaa catccagaga atctgtaaca ctaaccgcca tcaaggatgg tttagctgct
      721 ttatggaaaa agtatgagga aactgtaaat aatcgcgatc gtgcaatgac attcggtcgc
      781 ttgtgggaaa aattcctcgc cagcgcctat tacagtgacc tcagtccaag aacacaaaaa
      841 gattatctgc aacatcaaaa aaagttgctt gccgtattcg gtaaggtgcc ggcagattcc
      901 ataaaaccag aacacatccg tcgatacatg gacaagagag gggaacagag taaaacgcaa
      961 gccaaccatg aaaaaagcag tatgtcccgc gtttacagtt gggggtatga gcgagggtac
     1021 gtgaaggcta acccatgtgc aggtgtaagt aaattcaagg ccaaaaaccg cgaacgatat
     1081 gtaaccgaca aagaatacca ggcagtatta agcgttgcac ctcttcctgt ttttatcgca
     1141 atggaaattg cctatctgtg tgcagcgagg gtttccgatg tgttatcgct gaaatgggag
     1201 cagattggaa acgacgggat ctttatccag caagggaaaa caggaaaaaa acagataaaa
     1261 gcatggagtc cacgattaca ggcggcgatc gaaaaagcaa aacagttacc aacatccgcc
     1321 tatgtaatca gcaatcaata cggcaaccga tatatgtaca aaggctttaa cgaaatgtgg
     1381 gtagaagcaa gaaatcgcgc aggcaaaatt tcaggtattt taaccgactt cacctttcat
     1441 gatctgaagg cgaaaggaat ttcagactat gaaggaagca gtcgggataa gcaacttttc
     1501 tctggtcaca aaaccgaggg gcaagtgcta atctatgaca ggaaggttaa agtttcaccg
     1561 acacttgatg tcccgttacc tgaaaatatt ccaagaaaat attccaagta attccaagtg
     1621 tgatttttgt cactgactta atgatgtata agtgattgaa ttttggcgga gagaggggga
     1681 tttgaacccc cggtggagtt gcccccactc cggttttcga gaccggtccg ttcagccgct
     1741 ccggcatctc tccgctcaga tggttgccat gatgccagga aatttggcat tttaacagtc
     1801 cctgtccgtg caattttgtt caagtgacga gtttgcgagc aaaacgatga ttaagtggcc
     1861 ctggaaagta caagaatcag cacatcaaac tgcccttccc tggcaggaag cactatcgat
     1921 ccccctttta acgtgtctga cagaacagga acaaagcaaa ttggtcgctc ttgccgaacg
     1981 ttttttacag caaaaacggc ttgttccttt acagggcttt gagctgaatt cattaagaag
     2041 ctgccggata gcacttctat tttgcctgcc cgttctggag ttaggactgg aatggctgga
     2101 tggttttcat gaagtcttaa tttatcctgc gccatttgtg gtcgatgatg aatgggaaga
     2161 cgatatcggt ctggtgcata accaacgtat tgttcagtca ggtcagagct ggcagcaagg
     2221 gcctatcgtt ttgaactggt tggatataca agattctttt gatgcttctg gttttaacct
     2281 gattattcat gaagtcgctc ataagctgga cacccgtaac ggcgatcgcg ccagcggagt
     2341 tccctttatt tcgttgcgtg aggttgctgg ctgggaacac gatcttcatg ctgcaatgaa
     2401 caacattcag gaagaaatcg aattagttgg tgagaatgcg gcgagcattg atgcttatgc
     2461 tgccagtgat cctgctgaat gttttgccgt actttctgaa tatttcttta gcgccccaga
     2521 actttttgct cctcgtttcc cttcattgtg gcaacgtttc tgtcaatttt atcaacaaga
     2581 tcctttgcag agactgcatc acgctaatga tacagactcg ttttcggcga cgaatgttca
     2641 ttaattaaca actttgcaga ttaattaacc aattgaaatg gcttatgaaa tttagtgttg
     2701 acagacaagg taccgctaag taatatgcgc cccgttctca cgattcctct gtagttcagt
     2761 cggtagaacg gcggactgtt aatccgtatg tcactggttc gagtccagtc agaggagcca
     2821 attttctgtt ttcatgcatc cttgcgaatc tttatctgat gttgattcaa caggttagtg
     2881 tgaaaactct tctcggtgca ttttgatttt accctacgca tcgggaaaaa ttggtggtca
     2941 aatctgggat caggttagtt cgataatgga gtgaccccca tatatccctt accgacgcaa
     3001 aaatccgcac cctcaagcct tctgataaac cctttaaagt ctccgattct cacggtctgt
     3061 atctgctggt caagccgggt ggctcccgcc actggtatct caaataccgt attagcggta
     3121 aagaatcccg cattgcgctg ggtgcctatc cagccatctc cctgtctgat gcgcgacagc
     3181 aacgtgaagg tatccgtaaa atgctggcgc tgaatatcaa cccggtacag cagcgggctg
     3241 ctgaacgtgg ctcacgaaca ccggagaaag tttttaaaaa cgtggcgctg gcgtggcata
     3301 aaagtaacag gaaatggtcg cagaacaccg ccgaccgtct gcttgccagc ctgaacaatc
     3361 acatctttcc ggtcatcggg aacctacctg tatcagaact taaaccccgt catttcattg
     3421 acctgctgaa agggatcgag gaaaaaggtc tgctggaggt tgcgtcccgc acacggcagc
     3481 acctgagtaa cataatgcgc catgcggtcc atctggagtt aatcgatacg aaccctgcag
     3541 caaaccttgg cggcgtgacc acacctcctg tcagacggca ctatcctgcc ctgccgctgg
     3601 agcggctgcc tgaactgctt gaacgtattg gggcatatca tcagggccgt gaactgaccc
     3661 ggcatgccgt tctgctgatg ctgcatgtgt tcattcgctc cagtgaactg cgtttcgccc
     3721 gctggtcaga gattgatttc acaaaccgag tctggacgat acccgcgacg cgagaaccca
     3781 ttattggcgt gcgttattcc ggccgcgggg caaaaatgcg aatgccgcat atcgtccccc
     3841 tctcagaaca gtccatcgcc attctgaaac agattaagga tatcaccggt aataatgaac
     3901 tgatcttccc cggcgaccat aacccgtata agccaatgtg tgaaaacacg gtcaataagg
     3961 cactgcgggt gatgggttac gacacgaaaa aggatatctg cggtcacggc ttccgggcaa
     4021 tggcatgcag tgcgctgatg gaatcgggtt tatgggcaaa ggacgcagta gaacgccaga
     4081 tgagtcatca ggagcgcaat accgtgcgca tggcttatat tcataaggca gagcacctgg
     4141 aagcccgcaa agcgatgatg cagtggtggt cggattatct ggaagcatgc cgagaatctt
     4201 atgcaccgcc ttatacaatt ggtaaaaata agtttatccc atagtatact aacgaatccc
     4261 acactctggc aaattaccac cacctctaca ggaggtggtt ctatatagac ttttatcaac
     4321 tttatgacga aaaacaccta
//
LOCUS       DADMGC010000218         4326 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.218, whole genome shotgun sequence.
ACCESSION   DADMGC010000218 DADMGC010000000
VERSION     DADMGC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.218"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..157)
                     /locus_tag="JNZ73_004032"
     CDS             complement(<1..157)
                     /locus_tag="JNZ73_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734102.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASK"
     gene            complement(402..932)
                     /gene="cybB"
                     /locus_tag="JNZ73_004033"
     CDS             complement(402..932)
                     /gene="cybB"
                     /locus_tag="JNZ73_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HAY8734103.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1121..2122
                     /gene="gap"
                     /locus_tag="JNZ73_004034"
     CDS             1121..2122
                     /gene="gap"
                     /locus_tag="JNZ73_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY8734104.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDNTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVMQLIRTLEKFAKL"
     gene            complement(2164..3603)
                     /gene="aldA"
                     /locus_tag="JNZ73_004035"
     CDS             complement(2164..3603)
                     /gene="aldA"
                     /locus_tag="JNZ73_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY8734105.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3800..>4326)
                     /locus_tag="JNZ73_004036"
     CDS             complement(3800..>4326)
                     /locus_tag="JNZ73_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAY8734106.1"
                     /translation="TTGRAEATIQVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
BASE COUNT         1144 a         1083 c          992 g         1107 t
ORIGIN      
        1 ttttcgatgc actaatagaa ccatcattac agacaaattt gccgcctgcc gtacagtgtg
       61 agatcccccc tttcgaacca gaacagggtt tgcgcccggc atcggcataa aaggaaacaa
      121 gcgttcccat aaacaaaata agtgccagtt ttttcatata acctccatgg tttatcgaat
      181 cttatgtgcg atttgaacgt tatacgctgg tttattttga taccgttgat gagatcaaag
      241 atcgggtgaa ttgattaaaa ataagccaga tggacgtatg ggcaaggatt atggttttat
      301 ttgtcataca aataaatatg ataggcgctt ccgatgtaga cccgtattct tcgcctgtac
      361 cacgggtcgg ttttagtaca ggcgttttct ttaaatatcc ttcaggaacg tttacgcggc
      421 atcatgcgta gaagtgtgtt gtccttccag aaatagtggt gtgccagtgc agccgcagcg
      481 tgcaacccga tgacaaaata tcccagattc gccagcgtct catgccacga ctttaagcta
      541 tcaacccgtt cgaaattggc ctctgaagcg taaggcatcg tcaaaccaaa cgcaaaccac
      601 gggttgcccc ggttatacat catcaccaaa ccaatcactg gcagcgcaat aaacaggaga
      661 taaatcacca aatgtcccaa atgcgccagt cccgtcatca tcggtttcgg cttaggtata
      721 atcggcgggg ttgggtattt cagtcttaac aaaagacgaa ccaccatcag cacgagaatt
      781 gagatgccac aggaaacatg aatcatgttg atgagcggcc gatcgctacg tgggaagaaa
      841 ccacgaaact ccattgcgca atacgctgcg ataaccagca aaaagaccag ccagtgaatg
      901 ctgatttgta accttgagta tttattttcc ataacatttc ctgctttaac ataattttcc
      961 gttaacataa cgggcttttc tcaaaatttc attaaatatt gttcacccgt tttcagtacg
     1021 ttacgtttca ctgtaagaat ggagattgcc catcccacaa tcctggtcta agcctggaaa
     1081 ggatcaattt tcatccgaac gttcctgaca ggagaaaacc atgagtaaag ttggtattaa
     1141 cggttttggt cgtatcggtc gactggtgtt gcgtcgttta cttgaagtca aaagcaacat
     1201 agacgttgtc gctattaatg atcttacttc cccaaaaatt ctcgcctatc tgctgaaaca
     1261 tgattcaaac tatggtccgt tcccctggag cgttgatttt acggaagatt cacttatcgt
     1321 tgatgggaaa agtatcgcgg tttacgccga aaaagaggct aaaaatattc cgtggaaagc
     1381 gaaaggggca gaaatcattg tcgaatgtac tggcttttat acctccgccg ataaatcgca
     1441 ggcgcatctt gatgctggtg cgaagaaggt gttgatttcc gctcctgccg gtgaaatgaa
     1501 aaccatcgtt tataacgtta atgacaacac tctggatggc aacgacacca ttgtttccgt
     1561 ggcgtcatgc accactaact gccttgcgcc gatggccaaa gccttgcatg acagtttcgg
     1621 gatagaagtc ggcacgatga cgaccattca tgcctatact ggcacccagt cactggtgga
     1681 tggcccgcgt ggtaaagatt tacgtgcttc acgcgcagcg gcagaaaata tcattcccca
     1741 cactacgggg gcggcaaaag ccattggtct ggtgatcccg gaactgagcg gcaaactgaa
     1801 aggtcatgcg caacgcgtgc cggtgaaaac aggttcggtc actgagctgg tgtccattct
     1861 cggaaaaaaa gtgactgccg aagaggtgaa taacgcactt aaacaagcaa ccaccaataa
     1921 cgagtcattt ggttataccg atgaagaaat agtctcttcc gatatcattg gcagccattt
     1981 cggttcagtg tttgatgcca cgcaaacgga aattaccgcc gtgggcgatt tacaactggt
     2041 gaaaacggtc gcctggtacg ataacgaata tggcttcgtc atgcagctta ttcgcaccct
     2101 cgaaaaattc gctaaactct gacgcgcaca ggcggaggaa aaaacctccg cctctttcac
     2161 tcattaagac tgtaaataaa ccacctgggt ctgcagatat tcatgcaggc catgtttacc
     2221 atctgcgccg ccaataccgg atttacgcca tccggcgtgg aaaccttgca tagcttcgaa
     2281 gttttcacgg ttgatgtaag tttcaccaaa cttcagccct ttaatggctt tcatcgcgac
     2341 gttcagattt tgggtataga ttgatgaggt caggccgtaa tcactgtcat tagccattga
     2401 gatagcatct tccagcgtgt caaatgcgac aactggcagc accgggccaa aggtttcctc
     2461 atgcataatc gacatttcct ggcgaacatc cagcagcaat gtcggcggat aataatatcc
     2521 tttcccctct accgctttgc cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc
     2581 cactttttgc tcaacccttt ccagcgccgc ggcgttaatc aacggcccca tcgcaatgtc
     2641 gttgcgttca gcggggttac caaattgaac cgcctgcaac gcttcaccca gccgattgac
     2701 gaactgatca taaatgcctt tctgtacata aacacgttct gcacagttac acacttgccc
     2761 actattaatg acgcgtgaat caacgatggc tttgactgcc agttcaagat cggcatcgtc
     2821 cattacgata gccggtgctt taccacccaa ttccagacac actttggtga tgtttttcgc
     2881 cgcagtcgcc atgatcttct cacctgcaga gacgctgcct gtcatactga ccattgcgac
     2941 ctttgggtta cccgccagtt cttgcccaac ggtttcacca cgccccagta caaggttaaa
     3001 cacgccgcgc ggaaggccta tttcatcgac gattttggcg aatgcaatcg cattgtttgg
     3061 cgtaaattca ctgggtttaa tgacgatggt attaccggtc aaaagagcgg gagccatttt
     3121 gcgggcaatg aggaagaacg gaaagttcca cggcagaatg ccggtagtca caccaagcgc
     3181 acgtttaaac agaagaatat tttctcctgg acgatcgctt tgaataatct cgccctcgta
     3241 acgccgtgcc cactccgcca tgtaatcgat atagtcggca gtaaaagcca cttcgacttc
     3301 agctagctgc tggatcttgc ccccttcttc aacaatcagc gcactgattt cactggcgcg
     3361 ttcgcggatc ccggcggaga ttttgcacaa ccaactggcg cgttcaatag caggcaacgc
     3421 ttcccattct ggttgtgcac gttctgctgc atcgattgcc ttacgggcat cctcggcctg
     3481 accatcgggt atgcgggaaa tgacagcctc tgtagcaggg tttaccacat caatccatgc
     3541 gtctccacgc caggtaacaa actgtccatc gatatacata ggatgttgaa cgggtactga
     3601 catgggcgac tcctgtgatt tatatgtttg ttttcggtga atatattgtt aattaacatc
     3661 aatcatcaga gagacggcat cgggaaagga caattctgtg gagggcttca taaaaacaag
     3721 tgtaaaagga ataaaaggca gaaataaaag tcaggagtca tgcccgtttg caaacaagtg
     3781 gttcacaaac gggcaatttt taacgtaacg agcgactttc catcgcctcg atgagcaccg
     3841 catcatgttt cagcgtttgc catgcatgga tgacttctgc cggaaaatca acgtgaacga
     3901 taaaatctcg cccgcgggga ccgtagccat cgctatcatc gtgtaaacgc ggcagctcgc
     3961 cagtgagtag tgagagataa cgctcaaccg gccataatcc ttgtaactga ttaataaaga
     4021 ttacactgtc tgcgttattt cctaactgag gaacgaatcc gggataactt aaccagcgtg
     4081 gtgcatcggg attgtccccg gtcatacggc ggaacgttgc catcgtgcgc cgctgcatgg
     4141 tggggtcctg tacaacgata gccgtatgaa ctcgttctac ggcctgattc aatagcgtga
     4201 tgctaaagcg tgcgttttca ccgcagtttg ttgactggtc ttcaatccag attttttcat
     4261 gcggaatatg ccagaactga tgagcgatat ccacctggat ggtcgcttct gctctgccag
     4321 tggtgc
//
LOCUS       DADMGC010000219         4273 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.219, whole genome shotgun sequence.
ACCESSION   DADMGC010000219 DADMGC010000000
VERSION     DADMGC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.219"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            158..827
                     /locus_tag="JNZ73_004037"
                     /pseudo
     CDS             158..827
                     /locus_tag="JNZ73_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1070..1765)
                     /locus_tag="JNZ73_004038"
     CDS             complement(1070..1765)
                     /locus_tag="JNZ73_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HAY8734107.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1758..3185)
                     /locus_tag="JNZ73_004039"
     CDS             complement(1758..3185)
                     /locus_tag="JNZ73_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HAY8734108.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3196..3915)
                     /locus_tag="JNZ73_004040"
     CDS             complement(3196..3915)
                     /locus_tag="JNZ73_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HAY8734109.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1146 a         1100 c          880 g         1147 t
ORIGIN      
        1 ccagatatgg ggaccgaatc gttatataaa acactgaggt aactctcatg cttcatatcc
       61 agtattcatg atgctggttt tttgctgata tttaacccga acattcctga tacagactat
      121 taatacaaca ttatcttcga cagattttag atatattatg agtgaacaaa tcaaacagga
      181 tatcgatctg attgagattt tatttcatct gaagaaaaag attcgtgtta tcctttttat
      241 tatggctatc tgtatggcaa tggtgctgtt gtttctgtat atcaataaag acaatacaaa
      301 agtgatttac agccttaaaa taaaccagac aacgccaggt atacttgtta gctgtgatag
      361 caataataat tttgcctgtc agaccacaat gactgaagat gttattcagc gaattactac
      421 attttttcag accagcccag atatcaagaa cagagaaata aggctggaat ggtcaggaga
      481 taagagagat ttaccaactg ctgaagagga aatatctcgc gtgcaggcgt ctattatcaa
      541 atggtatgcg tcagaatacc ataatggcag gcaagttctc gatgagatac aaacgccttc
      601 agcaattaac agtgagcttt atacaaaaat gatatacctg accaggaact ggtcattgta
      661 tccgaacggt gatggctgtg taactataag ctcaccagaa ataaaaaata aataccctgc
      721 tgccatttgc ctagctctgg gatttttttc taagcattgt aatttctgta atgttttgcc
      781 ttgtcaaaaa aatggtagat gaataccaac aaaactctgg gcagtaataa acagcattct
      841 gatagagagt gcctgtataa agctgaagtg acaatgaccg ggaagggaac ccgttttcag
      901 gaatggatgc ctgtcttttt cactgtaaat atcctggcat tactaatgcc tggatattta
      961 cagaagagca gcataaccat gacggaacgg ggttaagtca ggttgtcctt aacgctattt
     1021 tcaacctcgt atgccttctt caggtttatg ttcagatctc atatctctct cagcaatcct
     1081 caataatcag atacaccgct ttcaccggac catgaacccc gacgactttg ataagctcaa
     1141 tatccgccgt tgagctggga ccactgatga tgttaataca tgacggcatt cgttcaccgg
     1201 cctgcgcttt ctgatgcaat ttttctgcga gttgcgctac acgcggcagg attgtgcttt
     1261 tacgcaggat aaaaagagaa gattccggga gcaggctcaa tgaacgcccg cgctcggcgg
     1321 cggaaaaaag aaccacgcct cccgattcgg ttaaaccata ttcagcatac acgacaccca
     1381 ctttagcctg ctctgcctgc gagatattct cggcaccttt cgccggatcc caaacaacgg
     1441 cattgcattc ctgctgcaaa cgttcactaa tccccaattc ctccagcctc gtgtcaccgc
     1501 taatcacgac cgactgatct cccagctctt tacacagacg tattgcagct tctgccgcct
     1561 tcgcctcgct ggtcagctca cagcgcgtca acataacatc gctggcaaac tgaataaacg
     1621 cgtcacagcg ctgctgttgg ttaagttggg taagccgctc gttagcatag ttgttaagcg
     1681 gcgcatcttc tgcttgcggt tcaagtcgca gcgggcgacc cagtgcctga gcaacgttat
     1741 tcaaaaattc gctccgatta tccattcttt ttctcctgcg cctgatgttt cttaaaccaa
     1801 ctacggaaac tctctccgtc agcttcagga agatcgcgtg cttccatcca gtcgctaatc
     1861 gcgccaaatt tgagtggtgt tttaccgcca ttgataaacc agcttgccgc atgagcgccg
     1921 gccatcatcc cgactttcca caatcctgga tgactattgg cataagcgaa catttttatc
     1981 gcccgttgct ctgcttttgc ggtgatccct ttttcagcca tcacccgacg atgacgcaaa
     2041 atcagttttg acagcggaat acgcaccgga cacacgctgt cacaagcagt gcataaagag
     2101 caggcgtagg gtaaatcttt aaaatcttta tagccgccaa gtagcggaga aatcaccgca
     2161 ccaattggcc ctggataaat agagccatat ccatgaccgc caatatggcg atatgccgga
     2221 caagtattca tacaagcccc gcagcgaata cagcgcagca catcccgaaa ttcagaggcc
     2281 agcacctcag aacgcccgtt atcgacaata accagatgaa actcttcagg accatcaacg
     2341 tgccccgctt cgcgcggccc tgtcagccag gtgttgtatc ccgtcaaacg tgcaccaacg
     2401 gcactgcgcg ccagcatggt gatcaataca tctacctcgg caaacgtggg ggcaatacgc
     2461 tccattccca tcactgcaat atgcgtttta ggcagcgtgg tacacattcg cgcattacct
     2521 tcattggtca ccaggcatac cgaaccggtc tctgccaccg cgaaattaca gccggtaata
     2581 cctatttcag cactgaggaa atcttcacgg attttttgcc ggatgaataa ggtcatcgct
     2641 tcaggcgttt ccggcccctc atagcccaga cgttcgtgta gcactcgacg gatctgatgg
     2701 cgatctttat gaattgccgg gaccacaaca tgagatggcg gatcttgatc cagctggaga
     2761 atatattcac ccagatcggt ttcaatcacc tgaatgccag catcctgcaa cacatgattg
     2821 acaccaatct cttcggtcac catcgattta gatttcacca ccttccgggc atttttgcgt
     2881 tgggcaacct gtaaaatgta gcgggtagcg tcttctttgg tttttgcaaa atagacgtga
     2941 ccgccgtttt gcgtcacttt ttctgagagc tggtacagat aagcgtcgag attactcaaa
     3001 acatgatcac gtatctgggc ggcccgatcg cgccactcct cccagtgccc caattcatcg
     3061 accatttttt gccgatttgc cccaatacgc tgctgcgcgt ttgccaccgc tttgcgcatg
     3121 atcggatctt caatttgctg acggatgcgt gtcttaaaat ctgtattact ggttttgatc
     3181 gacatcttta tatcctcagc ggctcatcaa cacttcagca atatgcatca ctttgacttt
     3241 ctgcccttcc cgttgtaatc gcccactgat gttcagcagg caactcacgt cagcaccaat
     3301 taaatactca gggcggactt ccatcaggtg cacaaccttt tctttcacca tctcgccgga
     3361 tatttcggcc attttgaccg agaacgtgcc gccaaatccg cagcaggtat cctgttcagc
     3421 aaaggtcaac agctccagtc cacgcacatt tttcagcagc gtaagtggct cgtccttcac
     3481 tcccagctta cgggccaggc tacaagatgg gtgatacaca gctctccctt gcaaactggc
     3541 acctacatcg actaccccta atttattaac aataaaagag gtgagatcct gcatacgcgc
     3601 ggcaaccttt tcggcacgtg atgcccattc aggttcatcc gccagatacg tcgggtaact
     3661 ttttacggca taggtgcaag agccagccgg tgaaataatg ggatcgtcgt tatcctccag
     3721 tgcggcgatc agatttttca tccctggaat cgcttctttg atataaccgc tattgatcgc
     3781 aggctgaccg cagcatccct gtttctccgg gaaatttacg cgacagccga gtttttccag
     3841 tagcagcacg gagtctcgtg ccattcttga tttcagggcg tcaccaatac aggtgacaaa
     3901 gaaattgaca ttcacaacta atactccatt acttcatgcc catttatgcc cgatgtttcc
     3961 cccccaccgg gggatcaatc atcaataaat atggcgttct ttttaaattg gctagcaaca
     4021 taccacccct catttgtatg acaaatgata atctttcatc caaacttcaa atgcaataac
     4081 taattttatt tctttcggca tcgctcacat tttatgggga aatcgacgat catttttggc
     4141 ataagaaatc catttaaaaa tggctacatc ccttatacct attcagagct acttattcag
     4201 gggttattga gatatacact gatggtgtac aaaaaaatca ccgacaataa tcacgtatca
     4261 agtaatagtc gga
//
LOCUS       DADMGC010000220         4224 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.221, whole genome shotgun sequence.
ACCESSION   DADMGC010000220 DADMGC010000000
VERSION     DADMGC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.221"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            175..1832
                     /gene="dpiB"
                     /locus_tag="JNZ73_004041"
                     /pseudo
     CDS             175..1832
                     /gene="dpiB"
                     /locus_tag="JNZ73_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            1801..2148
                     /locus_tag="JNZ73_004042"
                     /pseudo
     CDS             1801..2148
                     /locus_tag="JNZ73_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2260..2985)
                     /locus_tag="JNZ73_004043"
     CDS             complement(2260..2985)
                     /locus_tag="JNZ73_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HAY8734110.1"
                     /translation="MPASIPRRNIVGKEKKSRILTKPCVIEYEGQIVGYGSKELRVET
                     ISCWLARTIIQTKHYSRRFVNNSYLHLGVFSGRDLVGVLQWGYALNPNSGRRVVLETD
                     NRGYMELNRMWLHDDMPRNSESRAISYALKVIRLLYPSVEWVQSFADERCGLAGVVYQ
                     ASNFDFIGSHESTFYELDGEWYHEITMNAIKRGGQRGEYLRANKERAVVHKFNQYRYI
                     RFLNKRARKRLNTKLFKVQPYPK"
     gene            complement(2906..3094)
                     /locus_tag="JNZ73_004044"
     CDS             complement(2906..3094)
                     /locus_tag="JNZ73_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY8734111.1"
                     /translation="MKSIRCKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTE
                     KHCGKREEITHSDETVRY"
     gene            complement(3188..3385)
                     /locus_tag="JNZ73_004045"
                     /pseudo
     CDS             complement(3188..3385)
                     /locus_tag="JNZ73_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344231.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3479..>4224
                     /locus_tag="JNZ73_004046"
     CDS             3479..>4224
                     /locus_tag="JNZ73_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8734112.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADSDTDSY
                     MWFETGDNGNEYFKWRHHLTGGRVKDLMNLKWDALYVLVKALFSSEVKISTVNALRIF
                     NSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRTGRISMGHGLVVTGDIF
                     TNRFLINSSTGMWIHMRDQNVIMGRNAVSNDGAQALLRQDHTDRKFVIGGLGNRQFGI
                     YMINNSRTANGTDGQAYMDNNGNWLCGSQV"
BASE COUNT         1090 a          937 c         1049 g         1148 t
ORIGIN      
        1 ttaatggttt taagtttttt aattaatgta attacgaaat gactcgcagg tttaagtgat
       61 ttaattgatt taatgaataa aatttgccac gatcataatt aatatctatg tattttgatt
      121 caacatttta attacatccg tcaaagaggc tcgggacaac ccgcaaggaa aacaatgttg
      181 cagcttaacg agaataaaca gtttgcattt ttccaaagac tgacatttcc gctgcgtatc
      241 tttttgctga ttctggtgtt ctcaatattt gtcattgcag ccctggcgca atattttacg
      301 gccagttttg aggactattt aacgcttcat gtacgcgaca tggcaatgaa tcaggcgaaa
      361 attattgcct ccaatgacag tgtcatctct gcggtgaaaa cgcgtgacta caaacggctg
      421 gcgaccatcg ctaacaaatt acaaagagat accgattttg attatgtggt gattggcgac
      481 cggcactcga tccgccttta ccatcctaat ccggagaaaa ttggttatcc tatgcagttc
      541 accaaacagg gcgcgctgga gaaaggggag agctacttca ttaccgggaa agggtcaatg
      601 ggtatggcga tgcgcgccaa aacgccaatc tttgatgacg atggaaaagt catcggcgtg
      661 gtgtcgattg gctacctggt gagtaaaatc gatagctggc gggctgagtt tttattaccg
      721 atggcaggtg tgtttgtcgt gctgttaggg attctgatgt tgctgtcgtg gttcctggcc
      781 gcgcatatcc gtcggcagat gatgggcatg gagccaaagc aaatcgcacg ggtggtccgt
      841 cagcaagagg cgctgtttag ttcggtttat gaagggctga ttgcggtgga tccgcacggt
      901 tacattaccg ccatcaatcg taacgcaaga aagatgctgg ggctgagttc ccctggacgg
      961 caatggttgg gtaaacccat tgctgaagtg gtcaggtctg ccgatttctt taccgaacag
     1021 attgatgaaa aacgtcagga tgtggtggct aactttaacg gtctgagcgt tattgccaat
     1081 cgggaagcta ttcgttcagg tgatgatttg ctgggagcca ttatcagctt tcgcagtaaa
     1141 gacgaaattt ccaccctcaa tgcgcaactg acgcaaataa aacaatacgt tgagagcctt
     1201 cgtacattgc gacacgagca tctcaattgg atgtcgactc tcaatggtct gttgcagatg
     1261 aaagagtatg atcgcgtgct ggcgatggtg cagggggagt ctcaggccca gcaacagctt
     1321 attgacagcc tgcgcgaggc gtttgccgat cgccaggtgg cggggctgct ttttggtaaa
     1381 gtgcagcgcg cccgcgaact ggggctaaaa atgatcatcg tccccggtag ccagctttcg
     1441 caactgccgc caggactgga tagcaccgag tttgccgcca ttgttggcaa tttacttgat
     1501 aacgccttcg aagccagcct gcgtagcgat gaaggaaaca agagcgttga attattcctc
     1561 agcgatgaag gcgatgatgt ggtgattgaa gtcgccgatc agggctgcgg cgttccagag
     1621 tctctacgag acaaaatatt tgagcagggg tcagtacgcg tgctgacgag cccggtgaac
     1681 atggcattgg gttgtacttg attgccagct acgtaacgcg ctgcggtggt gttatcactc
     1741 tcgaagataa tgatccctgc ggtaccttat tttcaatcta tattccgaaa gtgaaaccta
     1801 atgacagctc cattaaccct attgatcgtt gaggacgaaa cgccgctggc agagatgcat
     1861 gcggaatata ttcgtcacat tcccggattc agtcagatat tactggcggg aaacctggcg
     1921 caggcccgaa tgatgatcga gcgttttaag ccggggctaa tcttgctcga taactatctt
     1981 cctgacggta gagggattaa tttactgcat gaactggtgc aggcgcatta tcccggcgac
     2041 gtggtgttta ccactgcagc cagtgatatg gaaacggtgt ctgaagccgt acgttgtggt
     2101 gtatttgatt atctcattaa gccaattgcc tatgaacggc tgggtgaagc ggcgcacgaa
     2161 aaacgcgaaa gcgtttcacg ataaatgcga aaactttagc tttcgcgctt caaatgaaac
     2221 agcagtatta attaccgctt tttattcatt acatggggat cacttagggt atggctgaac
     2281 cttgaatagt ttggtattta gccgcttcct tgctcgcttg ttcaggaatc tgatgtagcg
     2341 atactgatta aatttgtgca ccacggcacg ctccttatta gctcgcaaat actcgcctcg
     2401 ttgccctccg cgcttaatcg cgttcatcgt tatctcgtga taccactcac catccagctc
     2461 gtagaacgtg ctttcatggc tgccaataaa atcaaaattc gacgcctgat acacaacgcc
     2521 tgcgagtccg cagcgttcgt cagcaaagga ctgaacccac tccactgacg gatacagtaa
     2581 tctgataact ttcagcgcgt agctgatggc ccgtgattca gagttgcggg gcatgtcgtc
     2641 gtgtagccac atgcgattca actccatata gccccggtta tccgtttcaa gcacgacacg
     2701 acgacctgag ttggggttaa gggcatatcc ccactggaga acgccaacca gatcacgccc
     2761 gctgaatact cccaggtgaa ggtaagagtt attcacaaaa cggcgggaat agtgctttgt
     2821 ctgaatgatt gtgcgggcca gccagcagga tatggtttca acgcgcagct cctttgaacc
     2881 atagccaaca atctggcctt catactcaat aacgcacggt ttcgtcagaa tgcgtgattt
     2941 cttctctttt cccacaatgt ttctccgtgg gatgctcgca ggcattcagc attatgatgt
     3001 gacgtttgca acgcggacac ctgatttcaa tgtgatcaaa ggaatccgct ttaaataaca
     3061 gtttgttgca gttcttacag cgaattgatt tcatttcacc tccttgcatc aattcgccgt
     3121 tatcttaaaa aatctcatca gtcgggtgtg gttatcggga cataatcgat cggtaataca
     3181 gatcgattta attgattcga tcgtcgtttt ctatatgcgt tcctttttga aggcgcttta
     3241 aagcacgttt taatcccggt cggtctgtcc ttgttccgct taatttatct tcaaatattt
     3301 attcacatcc tgcacaaaca agagcgtttc gttgcaggtc tgtattctgg tcatttgttg
     3361 atacccttac atagccaatc agcacgctga attccccgtt taaaagcgga aatcatgcca
     3421 tgcaggccag aaacggcccc tcggtttaca ggaaacggta aacaaggctg gtaacgccgt
     3481 gcaaaaaaat ggcgatacgt tgtccggtgg acttactttt gaaaacgact caatccttgc
     3541 ctggattcgg aatactgact gggcaaagat tggatttaaa aatgatgccg acagcgatac
     3601 tgattcatac atgtggtttg aaacaggcga caacggcaat gagtatttca aatggagaca
     3661 ccacctcact ggcggccggg ttaaagacct gatgaatctt aaatgggatg ctctgtatgt
     3721 tcttgttaaa gcccttttca gcagtgaagt aaaaatatcg acggtcaatg cactgagaat
     3781 atttaattca tcttttggtg ccatttttcg tcgttctgaa gaatgcctgc atatcatccc
     3841 tacacgagag aatgaagggg aaaatggtaa tatagggcca ctacgcccct ttacgcttaa
     3901 tctcagaact ggccggataa gcatggggca tggtctggtt gttacaggag atatatttac
     3961 aaaccgtttt ttaattaaca gcagtacagg catgtggatt catatgcgtg accagaacgt
     4021 tattatgggc cgtaatgcgg tatccaacga tggtgcgcag gcattgcttc gccaggacca
     4081 taccgaccgt aaattcgtaa ttggtgggct ggggaatagg caatttggca tctacatgat
     4141 taataactca aggacagcca atggcaccga tggtcaggcg tacatggata ataacgggaa
     4201 ctggctttgc ggttcgcaag ttat
//
LOCUS       DADMGC010000221         4197 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.222, whole genome shotgun sequence.
ACCESSION   DADMGC010000221 DADMGC010000000
VERSION     DADMGC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.222"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..158)
                     /locus_tag="JNZ73_004047"
     CDS             complement(<1..158)
                     /locus_tag="JNZ73_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY8734113.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="JNZ73_004048"
     CDS             complement(308..469)
                     /locus_tag="JNZ73_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734114.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(466..669)
                     /locus_tag="JNZ73_004049"
     CDS             complement(466..669)
                     /locus_tag="JNZ73_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAY8734115.1"
                     /translation="MVERTMAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAIT
                     PYTLMQAIAAEGDVVVSGATEPE"
     gene            914..1249
                     /gene="iraM"
                     /locus_tag="JNZ73_004050"
     CDS             914..1249
                     /gene="iraM"
                     /locus_tag="JNZ73_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY8734116.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1661)
                     /locus_tag="JNZ73_004051"
     CDS             complement(1530..1661)
                     /locus_tag="JNZ73_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 domain-containing protein"
                     /protein_id="HAY8734117.1"
                     /translation="MIAYCFCEWHCSSRIMSVPADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(2161..2237)
                     /locus_tag="JNZ73_004052"
     tRNA            complement(2161..2237)
                     /locus_tag="JNZ73_004052"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="JNZ73_004053"
     tRNA            complement(2339..2414)
                     /locus_tag="JNZ73_004053"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2558..3379)
                     /locus_tag="JNZ73_004054"
     CDS             complement(2558..3379)
                     /locus_tag="JNZ73_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY8734118.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3394..3750)
                     /locus_tag="JNZ73_004055"
     CDS             complement(3394..3750)
                     /locus_tag="JNZ73_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY8734119.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3763..>4197)
                     /locus_tag="JNZ73_004056"
     CDS             complement(3763..>4197)
                     /locus_tag="JNZ73_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY8734120.1"
                     /translation="PGEQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPES
                     PESFMKRPKRRSWVNEKYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTL
                     PLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1159 a         1134 c          825 g         1079 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaacc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccactatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgc
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattgca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccattc acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaac
     2461 ccgctcagcg gcgggttgta gaaactcttc taacgtcagg cataaaacgc ccatcattat
     2521 gacgaattta ccacagattc cggaaaaatc aaccttgtta cctagttacc ttttttaact
     2581 gccgctcagc ccatgcttct tcaatatcaa accgggtcac cagcgcatca taaaatttct
     2641 taactgtttt ttcccatgac gcgcgtgtta tctggtttgt cacctcgcat atagcattaa
     2701 atgcctccgt tgatggtagt ctttcatagc cacgaccacc acaacgctgg cagtctctga
     2761 taacaggcat accacgtttt accgactctt cacgatgaat ggcgacacca cgcccacggc
     2821 aatccttaca ggcggtggaa acctcaccct ttccgccaca ctccggacag gcaactttta
     2881 ccacctccct gacttttttc cattcttccc agtaagacgg atacacacct ttcgtacact
     2941 ttgcccatac cggcggctta ccatccggat actggatctt gtttgtaaaa actatgcttt
     3001 caataaattt ttccccatag caacaagggc actgcttttt actcgctgcg ctgcgggcat
     3061 aatcctcaaa agcgtacgaa gccataatgc gcatcactac cggttttatt tctgccggaa
     3121 gttttctcaa cgccgccaca cgatcgcacc gactgagtgc ataatctgcc agtaattctg
     3181 ttgcccgcgc cctgtcattc atactgatgc ccattttccc caggaacgca gaaaaaccca
     3241 tctcagcccg attctgtgtc atgccctgcg cggccatcac atcagtgata ctcagcgcat
     3301 cttttgacgt tgaggccgat gcatcggtca ggccagggga ttttggggag tagtatttcg
     3361 gtaaatcttc cagtttcatt ttttgacctg ctcttaatgc attatggggt aaatcttcac
     3421 ccccagacgt ccaccagata ctggctgacc acgaacgata ttgatttcat caaactgctc
     3481 atcgtccatt aacactcccg catgcgtcag cgcatccagc ggtgctttca ggatattgtc
     3541 caggtcgcga cgacgcttat ccggtggctc tgcaatcacc tttatcgcca gccttccgga
     3601 caggcttaat ttcagccgct gctggcgaac aataagcgcc acagcccggc gataacgctt
     3661 tccctcctcc gagataaaat atgtgctgcc acggcgtcgc cagtaagtgt tcaccgtcgg
     3721 cgggtaaggt aaaaccaaat ctatgagcat cagtcacctc ttttacccaa gcacgccagt
     3781 tgcaaaggcg tgatcaagaa aacgaaaaat taaatcaacc tgagaaccat gcttttcttc
     3841 gaacgccaga ggatccgcat gaagctcgtt gtgatgctcc cgacacagcg gtagcgtgaa
     3901 aatatcgtga gattttgtcc ccattccgcc ctgaccatga ccaatcaggt gatggggatc
     3961 gtcggctggc ttaccacaac acgcacacgg ctgtgtcttc acccagcgtt tgtatttctc
     4021 gttaacccag ctgcgacgtt taggtcgttt catgaaagat tccggagact caggatcaac
     4081 ggcaatgctg accaccgtct tttcctgttg tgggttctgt tgctggtggg cgtgaggcag
     4141 cggcgcaaga ttttttgtgc gctgtttcag tatgctggtg gcggtctgct ctcccgg
//
LOCUS       DADMGC010000222         4169 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.223, whole genome shotgun sequence.
ACCESSION   DADMGC010000222 DADMGC010000000
VERSION     DADMGC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.223"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            259..852
                     /gene="tetC"
                     /locus_tag="JNZ73_004057"
     CDS             259..852
                     /gene="tetC"
                     /locus_tag="JNZ73_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HAY8734121.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            complement(965..2170)
                     /gene="tet(B)"
                     /locus_tag="JNZ73_004058"
     CDS             complement(965..2170)
                     /gene="tet(B)"
                     /locus_tag="JNZ73_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HAY8734122.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            2252..2875
                     /gene="tetR(B)"
                     /locus_tag="JNZ73_004059"
     CDS             2252..2875
                     /gene="tetR(B)"
                     /locus_tag="JNZ73_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HAY8734123.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(2853..3539)
                     /locus_tag="JNZ73_004060"
     CDS             complement(2853..3539)
                     /locus_tag="JNZ73_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY8734124.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(3547..3933)
                     /locus_tag="JNZ73_004061"
     CDS             complement(3547..3933)
                     /locus_tag="JNZ73_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HAY8734125.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            complement(3926..4141)
                     /locus_tag="JNZ73_004062"
     CDS             complement(3926..4141)
                     /locus_tag="JNZ73_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY8734126.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
BASE COUNT         1371 a          878 c          746 g         1174 t
ORIGIN      
        1 ggtataaccc gctttattcg ccacatcatc aagcagtaaa gactgatcta aattatgttc
       61 aatccataat agaacatctt tgataaccgt tgtatgaaac tgcttatcat catatcttaa
      121 ttggatgtta ttagctttat tttgatagcg agaatgctgt tcaatataca taaaataacc
      181 taaatgttct taagattgtc acgaccacat catcatgata ccataaacat actgacggta
      241 tgttatttta aatctatcat ggaaaataaa aatcatcaac aagaaaattt taagagtacc
      301 tatcaatcac tggttaactc agcacgaata ttgtttgttg aaaaaggcta tcaagctgtt
      361 tcaatagatg agatctcggg aaaagcgttg gtgaccaaag gtgcctttta tcatcacttt
      421 aaaaataaaa aacaattact cagtgcctgt tataagcagc aattaattat gattgatgcc
      481 tacatcacaa caaaaactga tttaacaaat ggttggtctg ccttagaaag tatatttgaa
      541 cattatcttg attatattat tgataataat aaaaacctta tccctatcca agaagtgatg
      601 cctatcattg gttggaatga acttgaaaaa attagccttg aatacattac tggtaaggta
      661 aacgccattg tcagcaaatt gatccaagag aaccaactta aagcttatga tgatgatgtg
      721 cttaaaaact tactcaatgg ctggtttatg catatcgcaa tacatgcgaa aaacctaaaa
      781 gagcttgccg ataaaaaagg ccaatttatt gctatttacc gcggcttttt attgagcttg
      841 aaagataaat aaaatagata ggttttattt gaagctaaat cttctttatc gtaaaaaatg
      901 ccctcttggg ttatcaagag ggtcattata tttcgcggaa taacatcatt tggtgacgaa
      961 ataactaagc acttgtctcc tgtttactcc cctgagcttg aggggttaac atgaaggtca
     1021 tcgatagcag gataataata cagtaaaacg ctaaaccaat aatccaaatc cagccatccc
     1081 aaattggtag tgaatgatta taaataacag caaacagtaa tgggccaata acaccggttg
     1141 cattggtaag gctcaccaat aatccctgta aagcaccttg ctgatgactc tttgtttgga
     1201 tagacatcac tccctgtaat gcaggtaaag cgatcccacc accagccaat aaaattaaaa
     1261 cagggaaaac taaccaacct tcagatataa acgctaaaaa ggcaaatgca ctactatctg
     1321 caataaatcc gagcagtact gccgtttttt cgccccattt agtggctatt cttcctgcca
     1381 caaaggcttg gaatactgag tgtaaaagac caagacccgc taatgaaaag ccaaccatca
     1441 tgctattcca tccaaaacga ttttcggtaa atagcaccca caccgttgcg ggaatttggc
     1501 ctatcaattg cgctgaaaaa taaataatca acaaaatggg catcgtttta aataaagtga
     1561 tgtataccga attcgattgc gtctcaaccc ctacttcggt atctgtatta tcacgtgtat
     1621 ttttggtttc acggaaccaa aacataacca caaggaaagc gacaatattt agcaacgcag
     1681 cgataaaaaa gggactatgc ggtgaaatct ctcctgcaaa accaccaata ataggccccg
     1741 ctattaaacc aagcccaaaa cttgccccta accaaccgaa ccacttcacg cgttgagaag
     1801 ctgaggtggt atcggcaatg accgatgccg cgacagcccc agtagctcct gtgatccctg
     1861 aaagcaaacg gcctaaatac agcatccaaa gcgcacttga aaaagccagc aataagtaat
     1921 ccagcgatgc gcctattaat gacaacaaca gcactgggcg ccgaccaaat cggtcagaca
     1981 tttttccaag ccaaggagca aagataacct gcattaacgc ataaagtgca agcaatacgc
     2041 caaagtggtt agcgatatct tccgaagcaa taaattcacg taataacgtt ggcaagactg
     2101 gcatgataag gccaatcccc atggcatcga gtaacgtaat taccaatgcg atctttgtcg
     2161 aactattcat ttcacttttc tctatcactg atagggagtg gtaaaataac tctatcaatg
     2221 atagagtgtc aacaaaaatt aggaattaat gatgtctaga ttagataaaa gtaaagtgat
     2281 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
     2341 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc
     2401 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt
     2461 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt
     2521 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta
     2581 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc
     2641 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga
     2701 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt
     2761 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct
     2821 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt cttaaaagca
     2881 gcataacctt tttccgtgat ggtaacttca cggtaaccaa gatgtcgagt taaccaccct
     2941 ttagattcat aaagcgaaaa taatgcggct ccaacgtacc cacctaaatg gaaacggcgt
     3001 tcactccaat ctaaacacgc acaacagatt ttacgtgaat gtttggaagg aacgtcaatt
     3061 cccatttcat gaaaatattg aataccactt aatgtgatca ttgaaccatt ttcagtgatc
     3121 cattgctgtt gacaaaggga atcatagatc ttaacggcaa cttcgccagc taaatgatca
     3181 tagcaagtac gtgcttttcg taaatgcact ggcgtggaaa ctttggcatg tacgccatgg
     3241 tttaaggaga tccccatcat actttccatc aattcagcaa tatcttttcc tgctagccga
     3301 aaataacgat gcttgccttg agctactact gtgattagct ggcaatctaa taatttagat
     3361 aaatgactgc tcgccgttga agctgatata ttcgccacag aacttagctc agtggccgtc
     3421 caagctcgcc catccatcaa agcactgagt attttaactc gtgaaatgtc agacatagcc
     3481 gcccctatcg cggctattga ggactcaaag gtaacctctt ttcgtattaa attagccatc
     3541 gcaagttcac tttattgccc aagggagcgt aacagatgca gccatactat cattgtccgt
     3601 tattaatatc agttggttag catggtcact gtattgcact aaaatattaa tgttattctc
     3661 cgccaatact cgtgctattt cgccaagttc ccccggtttt tcctgtttta acttacgaat
     3721 taatggtgtc cggatcgcaa gtactaacag tccagcttgc tctagcgcta ttttagcttc
     3781 ctttccttgt tcaacaagaa aatgagcatg gcattcatca ccaaccgtaa atatccctcc
     3841 cccttccaat ccaatacctt tattacctaa tgtttttcct agtaatgcta attgtcctat
     3901 ttgattatct aaaacaacgt gaacatcaaa cattatcctt gtccttttta aatgaatatt
     3961 cgcgagtaat atgagcaata ctaattttgt aaaaatcaaa tattgactct cgcccttctt
     4021 gttgagcttt cgcatgtaaa acatgatttt tccattgcag aactgcgtat tcgttttccc
     4081 accaagatag cgataacatt tttccttctg tagctagact ttgaaaacgt tcaattgaaa
     4141 taaaaccagc tacatgactt aatagtggt
//
LOCUS       DADMGC010000223         4073 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.224, whole genome shotgun sequence.
ACCESSION   DADMGC010000223 DADMGC010000000
VERSION     DADMGC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.224"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..111
                     /locus_tag="JNZ73_004063"
     CDS             <1..111
                     /locus_tag="JNZ73_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HAY8734127.1"
                     /translation="QAQVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(312..1169)
                     /gene="sitD"
                     /locus_tag="JNZ73_004064"
     CDS             complement(312..1169)
                     /gene="sitD"
                     /locus_tag="JNZ73_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HAY8734128.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1166..2023)
                     /gene="sitC"
                     /locus_tag="JNZ73_004065"
     CDS             complement(1166..2023)
                     /gene="sitC"
                     /locus_tag="JNZ73_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HAY8734129.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2020..2847)
                     /gene="sitB"
                     /locus_tag="JNZ73_004066"
     CDS             complement(2020..2847)
                     /gene="sitB"
                     /locus_tag="JNZ73_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HAY8734130.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2847..3737)
                     /gene="sitA"
                     /locus_tag="JNZ73_004067"
     CDS             complement(2847..3737)
                     /gene="sitA"
                     /locus_tag="JNZ73_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HAY8734131.1"
                     /translation="MLLGCLALTCSITFQASATEKFKVITTFTIIADMAKNVAGDAAE
                     VSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSS
                     GVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKA
                     KITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQV
                     RKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYID
                     LLKVTTSTLVQGIKAGKREK"
BASE COUNT         1108 a          996 c          996 g          973 t
ORIGIN      
        1 caggcgcagg tcgatcgcaa cgatgagatt tatcgccgtc agcgggagct gaaggaagag
       61 ctgaatagtc tgaaggattt gaattcggtt aggaatttta tcgtggaata acagaagctg
      121 cggcacgtcg tatgcaggaa cgtgccgcgg ttggctggta aactttcgat agtgtgagta
      181 ttgaatgatt tctaggcgtt atcgatttta cgtatttttt gcatgataga attcgtacct
      241 cctcccaacg accttccatg gctttccgga cttctgtagg ctggcggacg atgctgtcct
      301 cggatgttgt gctaattggg tgataacaga tcctgtgtat gggcgtttcc ctttgtgcgt
      361 gcgttgatgc tggtaacggt aaacgtcatg atgaacacga ccgcgaataa gacgacaatc
      421 gtcggagctg gtgcgctgtc gataaaaaag gacagccaga caccagtcat tgacactatt
      481 accgatatgc cagtcgccag taggagcgca atatgaaagc gttgtgtcag gagtactgca
      541 atggcgccag gcgcaatgag cagagaaata gagagaatga tacctactgc tttcagcgtc
      601 gccacaatgg tcagggaaac catgcacagc agcccataat gcagccatct cgtatgcaaa
      661 ccgcttacct gcgcctgttg ataatcaaag ctgaacagca aaaaatcccg ccattttacg
      721 ctaataacaa gtgtaaccag cccggcaata atcaccgtct ggattatatc gccgatggtt
      781 atcccgagca tgtcgccgaa aagaatatgg tcaagatgta cgtctggttt aattgcaata
      841 tacaagatca gacctgcggc aaacatgcca gaaaaaacaa tccccataac tgtatcttgc
      901 ttgattcggc tgttgtcttt caggtatccc gtcgccaccg cgcagaatac tccggcgacg
      961 aatgcccctg tagccagagg taaccccaga atccaggtaa gcacgatgcc aggaaaaacc
     1021 gcatgactca tcgcgtcacc catcagtgcc caacctttca ggaccagaaa gaccgacagc
     1081 agcgcacagg gtatggaaac taccagtgaa atcagcagtg cgtgactcat aaaggtgaac
     1141 tgcagtggct ccagtaataa tgccatcatg agcgatcctc cagcgcttta tgcgcacgcc
     1201 gacgatttgc caaaagtcca tgggtgggcg caaatacaaa tgctaaaaga aacagcagtg
     1261 tctgcgccac cacgatgatt ccacctgtcg cgccgtccag aaagtaactg acccacgcgc
     1321 cgaggaaact ggttacactg ccaatagtta cagcaattat gagcaggcgg ggaaaacgat
     1381 cggttaacaa ccatgctgtt gcaccgggag tcaccaccag acagatcacc agaaaggcac
     1441 caaccgtttg cagcgccgca acggtcgaaa ccgacagcag ggtgaagaag aggattttta
     1501 atctgcccgg atgtaatcca atagcacggg cgtgattctc gtcgaaaaag gtcaccataa
     1561 gatctttcca tttgaaaaaa agcacgatga tcgagagaat gccaatgata gtgagttgca
     1621 ggatatccgc cggatcgatt gccagaatat tgcccaggac gatggtctga atgttcacgg
     1681 atgtcgggtt caatgacacc ataaacagcc ccaggccaaa aaaggacgag aagatcaggc
     1741 cgatgatggc atcttctttt aagcgggtgc gttggttaag aaacagcatg ctgcccgctg
     1801 ccagtccgcc ggaaaagaag gctcccagag aaaacggaag ccccagcata tatgcccccg
     1861 caacacccgg gacaatagag tgcgacagcg catcgccaat cagcgaccag ccttttagca
     1921 tcagatagca tgacaggaaa gcgcagagac cgccgaccat tgccgagacc cacatcgcat
     1981 tgagcatata ttcatagctg aagggttcca gtagcacgtt catcttgctt ccctctgcac
     2041 cgctgacggt cgatgtgcca caaaagggcg ttcatcgtca gtaataatgc tttcttccga
     2101 gccgttaagt gtgacatggc gcagtacgcc gctaaaagcc agctccaggt tctcggctgt
     2161 aaaagtggtg tctgtcggcc cgcttgccag gacggtgcct ttgaccatga ccgtataatc
     2221 gcaaaatgtc gtgactgagc cgagattgtg cgttgagaca agcatcgttt tgccttccgc
     2281 gcgtaactcc ctcaacaggc tgatgatttt cgcttcggtt ttcacatcaa cgccggtaaa
     2341 aggttcatcc agcaggatca catcaccctg ctgtgcaatc gctctcgcca gaaagacgcg
     2401 tttcttttgt ccaccggaaa gctcgccgat ttgacgatgg cgaaaatcca ccatatcgac
     2461 acgttccagc gcatcggtga caatctgacg atccctcttt ttggctatac gcaacatacc
     2521 catatgccca tagcggccca tcatcaccac atcttccacc agtacaggaa atgaccagtc
     2581 aacctcttct gactgaggga cgtaggcaac caggtttttc tgtagcgcct gtcgcgtggg
     2641 aatacccaga acagatatct ttccgctggt cagacgcaca aatcccataa tcgctttaaa
     2701 caacgtcgat tttccggaac cgtttacccc aaccagagcg gcaatagatc cacttggtac
     2761 tgtgaaggat gcatcgcgca gtgctgtgtg cccgttacgc caggtgacag taacatcatt
     2821 aacgacaatg cctgcagatt gcatcattat ttctcccttt tcccggcttt aattccctgt
     2881 accagcgtac tggtagtaac cttaagaagg tcgatatacg taggtaccgg gccgttttct
     2941 gtgctcaggg aatcgacata gagcacaccg ccgtagtgtg cgccggtttc acgcgcaacc
     3001 tgacgcgctg gtttatcgga aatcgtactc tcgctaaaga ctgccgggat atgatttttc
     3061 ttaactatat caacaacctt acgtacctgc tgcggtgttc cttgttgatc ggcattaatc
     3121 ggccacagat aaagctcttt tagccccaaa tcgcgtgcga gataagaaaa agccccttca
     3181 ctggtgacca tccatcgctg attctcaggg agttccgtaa tctgcttacg caggggggca
     3241 agggtttggg taatcttggc tttataagta tcggcattac gttggtaggt ttgtgcattt
     3301 gccggatcgt attttatcag cgcatcacga atattatcga cgtaaatcag agcattatct
     3361 ggcgacatcc atgcatgggg attaggtttg ccctcatagg gtccttcggt gatccctact
     3421 ggcgtcacac ccgaagagac aattacttct ggaaccccat tgagatgctg gtaaaagcgt
     3481 tggaaccaca attccagatt cataccattg gcgagaatca gttgtgcccc ctgcgcacgt
     3541 ttaatatcgc caggggtagg ctgatactca tgaatttctg caccaggctt ggttatggat
     3601 gagacttctg cagcatctcc agccacgttt ttggccatat ccgcgatgat ggtaaatgtt
     3661 gtaatgacct tgaatttttc agttgcactt gcctgaaatg tgatcgagca ggtgagggcg
     3721 aggcacccca agagcatggt tacttttttt attgagtgca taatttagta cctatagtga
     3781 gcaaaattag aacaaacata gcaacggcta tgttttatag cataacctat acaaaatgaa
     3841 aataattatc attaacagct ttgaggccta aaacagggat taggttgttt cctgctggcg
     3901 acatgatatt gaggaaaaaa gtagagcagc tattaggctt tgaaatgact ttatgaggaa
     3961 aattgttgat ggcaaaaatg gggcaaaacg ctgtataagg ggcaaaaaag gggcaaaaaa
     4021 gagtgggtta tcgtagctta ttgttgtcgc tgataatgtt taacatattg aaa
//
LOCUS       DADMGC010000224         4058 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.225, whole genome shotgun sequence.
ACCESSION   DADMGC010000224 DADMGC010000000
VERSION     DADMGC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.225"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(1..394)
                     /locus_tag="JNZ73_004068"
                     /pseudo
     CDS             complement(1..394)
                     /locus_tag="JNZ73_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611999.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1084..2793)
                     /locus_tag="JNZ73_004069"
     CDS             complement(1084..2793)
                     /locus_tag="JNZ73_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAY8734132.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVANSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(3225..3944)
                     /locus_tag="JNZ73_004070"
     CDS             complement(3225..3944)
                     /locus_tag="JNZ73_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HAY8734133.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT         1171 a          837 c          745 g         1305 t
ORIGIN      
        1 gcatgctgta cccggtcgta ataaccgctc ctggatacac caggtacccg acacatcgct
       61 tcaacaggga attgttgtcg ccattgttcg actaacgcgt atttttcagc gactcctggg
      121 caaaatacgc tgttgctttt tttaatatat ctcgctcaag tcgtgcttca tttaaagcct
      181 tacgcagttg cataacttca gattccagct cagccactgt gcgggagcct ggtgtattga
      241 gcccttttct tgcggcggta acccattgtc ctaaagtgcc ttcaggaaga gataatcggg
      301 aagcgccttc actgatcgaa agctgatttt caggaaccgt tttgacggct tcggctttaa
      361 actccgtaga gtaacgttgg ttttttctgc tcattattgg ctccttctga tgccattcta
      421 tttcaggaag gagtgtccgt taaactcagg ctacctcaga taagcttgat acaactggtc
      481 tggtatatac agtttctttg agtgcggtta cgtccacaga ggtaacggta aaagtaaccc
      541 cttgtcggga ccactccgac cgcgcacaga ggcattttca tcgctgacag gccgcattgc
      601 ctcgcagggc tggaggagga tcggtgatat ccggacgacc gtaccggctt acgccgggct
      661 tcggcggttt tactcctctg ccatgtgcaa caacggagtg ccgccttaca tgccgctggc
      721 gcggcatatc ctggtaacat ttttgttgat accaccttcc ggccttatcc tgaccagtgg
      781 gctttcctgg catcgatgat accgatgaac gcgcatgata ttgagccgac gatattacgg
      841 gcaactggaa atactcactc tctggatgtg acctttatcc atgaagaaga tttggccaca
      901 ccatggaaac ctgaacaatc atcagtttac gcccatgtaa acccctacgg gatatttgaa
      961 ctcgatatgg aaacaaggtt gccaatcgag gtggttgcct gaaagttggc agttacccgg
     1021 tcactacacc ttgttggagt gaacatatca aaaacaggta attacacaat ttaaattaca
     1081 gtctcataaa aaatgattaa atctatttat tatttcaata aacgtttttt tcatcttctt
     1141 tgaaaattgt tctgctatag tatccggttc cagattgttc tcttttatta ttctacaata
     1201 ctctaatatt gtttctttgt ctctatttac tacagataca aatacaccag gaataccatc
     1261 tttattagtt gcggacagca tttcgaccaa tcgtcttgtg gtaaaactat agttttttag
     1321 aatattacaa aaggcacgta ttgtttcggt atggccatta taaaatgcca taaaaagtcc
     1381 tgatgtaccg ttgggatttt ttatccataa taattcatct atatattcct ggtgaggggg
     1441 gagtattttt agtatatctc catacgccat aatggccttt gtgtgcccat tttgaagtgc
     1501 cataaacaac ccatgcttta ctttcaataa ttctttaatt tgttcactac taagcatttt
     1561 cttattaagc agacttccga acatacgaat actatctgca tgtccattct gcaatgcaaa
     1621 aaataaccca ggtaaatcat ggctgtcttt cgcagctaat agatataatc tcttatcgga
     1681 agttaacggg gttttaaaga ctatttcccc ataggctttt attgtgtcta catgtccgtt
     1741 cataaaagac atgcataaac ccggcacatt gtttgcccct accgcagcaa gcaatgtaag
     1801 tattgtttct tgactaagat tgcacatatt taatatctta ccatatactt gaataatgtc
     1861 tgaatatccg ttttgaagtg ctatataaaa tgcaggagta ccatcagagc ttctcgtggc
     1921 cagaaggttc accagtttag attgagaaat ttcaccattt ttgtattttt ccatcagtat
     1981 aaataatact tctgtcagat tctttgttat cgcatgatat ataactctgg aattgatcaa
     2041 ttcatctggt aaaagtttca ctgtgtttgt tagaggggaa ttatgcctat cttcaaatat
     2101 tacctgcccg ggttgctcca gaccataaat tttccaataa aaatcatcaa aaaaatcctt
     2161 agcggataat tgactcagtt cgaaatggct atcacaagta aatacagtac gtttatgagt
     2221 aacagttcga ttaggatcga aaaactgaac aacccatttt tcactttctt gtacgcgctt
     2281 tattttcaac cctaacgcca tttggtggtt taatgtgcat gctgataatg ttctgacaaa
     2341 gtctccattt tttttcatgt tataaaaaaa attagtaata actgttcccc actttttatt
     2401 ttccacaaaa taaatataag ctggtgattt tgaaaaatct tccgtttttt ccaaacatac
     2461 attttttgca atagcatcag gggtgctaag aagctgatag tttaccttcc catttgtttc
     2521 cataaaattc atttcccaat aggaagctag gtgacggcaa actatttgtc ttccatatat
     2581 atcttttgcc actccattta tatttacctt cacatttctc ttttctgaaa aataaggtgc
     2641 tgttttattc ttaaactgaa ttagttcact ggagtttgcg accctcataa tagaacttgt
     2701 tattgttcta ttatctctgt taacagacat atctgttgat aacgaatttt ttgttgaaaa
     2761 tatactagat gaaaatgttt tatttaacgg catagaagac aaccatagag atgttataat
     2821 actattagat tgcagttact aatagtaaaa gcctccttac aaggaggtag cttattcaga
     2881 atgctcctgt aagatagcga gctcgtcaac gatacagatg atctgattat attgtgcaaa
     2941 aggagaacag gaaaataggc actggaaaag atgaaggcct gatgggagca ttaactaatg
     3001 caaaatccga caggttaata tcccgaaaag caatcaaacg actcaaacag gagatgcacg
     3061 ctgaagtggt ggacaactgt tcggatttca gatgagtagc tcagcagttt atgagccgaa
     3121 tgctctgtca cgggcaggaa tcaggctgca tacgcagtgc caaatctaat cgtagttatt
     3181 ctaaccagtt ttcaagctac aaggtggtgt agctggcatc ttctctactc tatcatcaaa
     3241 cgataaaatg gttcctctcg taacatttgc tcttgccttt cacttccatc tcgatcgctt
     3301 cgtagttcat tacgatagct gacatatttc cagtcttcag gtctaacatc tgacgccgga
     3361 tactccccgg gagcgatttt gcttcttaat agattagact cgagacacat tataaattgt
     3421 cgtattttat gaagtaataa agcactatat tgcgagcatt cctgatctga ttttacatat
     3481 agcgtaaact gagctcctat ccccacacga gattgctgag aaactcgatt catatcagtt
     3541 atcttccatt tatctatagg actgtcctct gaaaataata aaccagacaa aatttggaat
     3601 gccagaggaa cttgctccct agctatacta atatgaaact tgtccccaac aaaatccccc
     3661 ttactctgag gattttcacg acatgcatga ataaacacat ctccactatg gttcacaatc
     3721 tggaatcctt ggtacatgac agaaaaaaat tgacatatat cagagtaagt tggcacgttc
     3781 gatgctactt tgaaatagta cgctgggtat tgccgtattt ggttgtacaa tatattgaag
     3841 ttatttttca atctttcatt cgcagaaagc atctgcggta tttcagacct cacaacattc
     3901 aaagaatcta aatttagttt tagacagggc ttttttatgg gcatagaaaa cgtcctctat
     3961 aaaatagata tattatctta tattgattgt ttatcatctt ttataaaacg atacattata
     4021 tgtaatgtat gtaagcgtaa gggtcttctc cgatcatg
//
LOCUS       DADMGC010000225         3926 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.226, whole genome shotgun sequence.
ACCESSION   DADMGC010000225 DADMGC010000000
VERSION     DADMGC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.226"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(43..1872)
                     /gene="glmS"
                     /locus_tag="JNZ73_004071"
     CDS             complement(43..1872)
                     /gene="glmS"
                     /locus_tag="JNZ73_004071"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAY8734134.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2034..3404)
                     /gene="glmU"
                     /locus_tag="JNZ73_004072"
     CDS             complement(2034..3404)
                     /gene="glmU"
                     /locus_tag="JNZ73_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAY8734135.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGAGMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3756..>3926)
                     /gene="atpC"
                     /locus_tag="JNZ73_004073"
     CDS             complement(3756..>3926)
                     /gene="atpC"
                     /locus_tag="JNZ73_004073"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="part of catalytic core of ATP synthase;
                     alpha(3)beta(3)gamma(1)delta(1)epsilon(1); involved in
                     producing ATP from ADP in the presence of the proton
                     motive force across the membrane; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAY8734136.1"
                     /translation="AIRGQDLDEARAMEAKRKAEEHISSSHGDVDYAQASAELAKAIA
                     QLRVIELTKKAM"
BASE COUNT          901 a         1079 c          975 g          971 t
ORIGIN      
        1 gcattacacc ggtcggcagc ccttttttat ctatgatagt gattactcaa ccgtaaccga
       61 ttttgccagg ttacgcggct ggtcaacgtc ggtgcctttg atcagcgcga catggtaagc
      121 cagcagctgc agcggaacgg tgtagaagat cggtgcaatc acctcttcca catgcggcat
      181 ctcgatgatg tgcatgttat cgctacttac aaaacccgca tcctgatcgg cgaagacata
      241 caattgaccg ccacgcgcgc gaacttcttc aatgttggat ttcagttttt ccaacagttc
      301 gttgttcggt gcaacgacga taaccggcat atccgcgtca atcagcgcca gcgggccgtg
      361 tttcagctcg cctgcagcgt aggcttcagc gtgaatgtaa gagatctctt tcagcttcaa
      421 tgcgccttcc agcgcgattg ggtactgatc gccacggccc aggaacagcg cgtgatgttt
      481 gtcagagaaa tcttctgcca gagcttcaat gcgtttatcc tgagacagca tctgctcaat
      541 acggctcggc agcgcctgca gaccatgcac aatgtcatgt tcaatggagg catccagacc
      601 tttcaggcga gacagcttcg ccaccagcat caacagcaca gttaactgag tggtgaatgc
      661 tttagtggac gccacgccga tttccgtacc cgcgttggtc atcagcgcca gatcggattc
      721 gcgcaccaga gaagaacccg gaacgttaca gattgccagc gaaccaaggt aacccagctc
      781 tttcgacaga cgcaggcccg ccagggtatc cgcggtttcg ccagactgtg acaaggtgat
      841 catcaggctg ttacgacgca cggcagattt acgatagcgg aattcagagg cgatttcgac
      901 gtcgcacgga atacctgcca gcgattcaaa ccagtagcgg gaaaccatac cggagttata
      961 agaagtacca caggcgagga tctgaatatg ctcaaccttc gacagcagtt cgtcggcgtt
     1021 cggtcccagc tcgcttaaat caacctgacc gtggctgatg cgcccggtaa gggtgttttt
     1081 tatcgcgttc ggctgttcgt agatctcttt ctgcatgtag tgacggtaaa tacctttatc
     1141 gcccgcgtca tattgcagat tggattcgat atcctgacgt tttacttccg cgccagtttt
     1201 atcgaagatg tttaccgaac ggcgagtgat ttccgcaata tcgccctctt caaggaagat
     1261 aaagcgacgg gtcaccggca acagcgccag ctggtcagaa gcgataaagt tttcgcccat
     1321 acccaggcca atcaccagcg gactaccaga acgtgccgcc agcagggtat ccgggtgacg
     1381 ggagtccatg atcactgtac cgtacgcacc gcgcagctgc gggatagcac gcagaacagc
     1441 ctcacgcaga gtcccgcctt gtttcagctc ccagttcacc agatgggcaa tcacttcggt
     1501 gtcggtttca gaaacgaagg tatagccacg cgcttttaac tcttcacgca gcggttcatg
     1561 gttttcgatg atgccattat gcaccaccac aatgtgttca gaaacatgcg gatgcgcgtt
     1621 agcttctgaa ggttcaccgt gggtcgccca gcgagtgtga gcaataccgg tgccgccatg
     1681 cagaggatgt tcttccgctg cctgagccag catctggact ttaccgaggc gacgcaggcg
     1741 agtcatatga ccttccgcat caacaacggc cagaccggca gagtcatatc cgcggtattc
     1801 cagacgacgt aaaccttcaa gaaggatttc tgctacatca cgttgcgcga tcgcgccaac
     1861 aattccacac atagtttttg attccgattt atatcgttgt cggtcaacct gtatgcccgt
     1921 tttttcgggc gccccgagcc ttgtagagag tggggttatt tttatagtta ctgcttgtgg
     1981 gagggagatt atgttatctc ctcatcccag atgaccggat tacccggcca gaattacttt
     2041 ttctttaccg gacgacgcca gccttctttc tgagtctgcg gcacacggct gatagctaat
     2101 gcattttcgc cgacattacg cgtcacagtt gtacccgcag caatggtcgc gcctttgcct
     2161 actgttaccg gggccaccag ctgagtgtcg gaaccgacaa acacatcgtc gccgataatg
     2221 gtcttaaatt tattcgcacc atcgtagttg caggtaatgg ttcccgcgcc gatgttaacg
     2281 ttatcgccaa tttccgcatc gcctaggtaa gtcagatgcc cggctttcgt gcctttaccc
     2341 agacgcgctt ttttcatctc aacgaagtta ccaacgtgtg caccttccag caactcagca
     2401 ccaggacgca gacgggcaaa cgggccaata gtacaggccg ctgccagatt cgcgtcttcc
     2461 acaacggtat acggactgat ttcgcaatca tcgccaatca cgctgttttt aatcacgcaa
     2521 ccggtgccga ttttcacgcg atgaccgaga gtcacgttgc cctcgatgat aacgttagta
     2581 tcaatttcaa catcgcgccc gtgagtaagc gtaccgcgca gatcaaaacg cgccggatcg
     2641 cgcagcataa cgcctgctaa cagcagtttt tcagcctgtt cggactggta aacgcgctcc
     2701 agacgggaga gttgcaggcg gttattcaca ccttctactt cgcttaaacg ttgcggatga
     2761 acggcgacga tttcacgccc ttcctgatac gccagcgcaa taatgtcggt gatgtagtat
     2821 tcgccctgag cattattgtt ggtcagcttc gccagccagc gtttcatacc tgcgccgttg
     2881 gcaatcagaa tgccggtgtt gatctcctga atctgacgct gctcgtcggt ggcatctttg
     2941 tgctcaacaa tgccggtaac tttgccattt tcacgggtga tacgtccata accggtcgga
     3001 tcatccagtt tcaccgtcag cagaccaatg ccaccctggg gtttagcatc acgcaggcgc
     3061 tggagtgttt cgacagagat cagcggcacg tcgccgtaga gcattaaaat gtcttcatca
     3121 tcggcaaaga aaggtgcggc ctgctgcatt gcatgacccg tacccagctg ctctgcctga
     3181 agcacccagt taaggttgtc gtctttcagc gcctgtttaa gcagatcgcc gccgtgaccg
     3241 tacaccaggt gaacgtgcgc tgcgcctaat tcattcgcag catcaatgac atgctgaacc
     3301 atcgctttcc cggcaagggt atgcagcact ttcggaagat cggaatacat gcgcgtgcct
     3361 ttgcctgcgg caaggatcac tacgctcata gcattattca acatacgcgt cctgactgta
     3421 atttgagaac gaatttaaac cgcttcacct tgaaaaaact acattttttt catcgtgaaa
     3481 tggacagagg ataaattgtt caataacgga ttatccccgc tgacgaaagt gccattttcg
     3541 accatcgtca cgccttttgt ctgcagaaaa ttaaggcagt accttcattt aagcgtcagg
     3601 tggatgtttt tgctcttatt tcgatcaatg aataaacggg aaaacagggg ggttttatat
     3661 cagcaggatc tatgtgaacg ctcttcagga cgggtcaacg cgtaaaaaaa agccagcctg
     3721 tttccagact ggcttttgtg cttttcaagc cggtgttaca tcgctttttt ggtcaactcg
     3781 ataacgcgca gctgcgcgat cgctttggcc agttccgcag acgcctgagc gtaatctacg
     3841 tcgccgtgag agctgctaat gtgctcttca gccttacgtt tcgcttccat ggctcgcgct
     3901 tcgtcgagat cctggccgcg aattgc
//
LOCUS       DADMGC010000226         3874 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.227, whole genome shotgun sequence.
ACCESSION   DADMGC010000226 DADMGC010000000
VERSION     DADMGC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.227"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..413
                     /gene="ospD1"
                     /locus_tag="JNZ73_004074"
     CDS             <1..413
                     /gene="ospD1"
                     /locus_tag="JNZ73_004074"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspD1"
                     /protein_id="HAY8734137.1"
                     /translation="WHGNADAARKYGESLLAAEIPKEEKLEVLAARNNAGESALFIAL
                     QEGHSAAIQAYGDFIKTFDLSPKETIKLLDVRDNEGLPGLFLAAGKGNIEAMMAYINI
                     CHHSGIKLTEIADRLNNNEQDMFNIISDKIQELF"
     gene            737..847
                     /locus_tag="JNZ73_004075"
                     /pseudo
     CDS             737..847
                     /locus_tag="JNZ73_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            925..1239
                     /locus_tag="JNZ73_004076"
     CDS             925..1239
                     /locus_tag="JNZ73_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734138.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            1521..2087
                     /locus_tag="JNZ73_004077"
     CDS             1521..2087
                     /locus_tag="JNZ73_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734139.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2292..2408)
                     /locus_tag="JNZ73_004078"
                     /pseudo
     CDS             complement(2292..2408)
                     /locus_tag="JNZ73_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989794.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            2614..3168
                     /locus_tag="JNZ73_004079"
                     /pseudo
     CDS             2614..3168
                     /locus_tag="JNZ73_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            3508..3874
                     /locus_tag="JNZ73_004080"
                     /pseudo
     CDS             3508..3874
                     /locus_tag="JNZ73_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1158 a          810 c          867 g         1039 t
ORIGIN      
        1 tttggcatgg aaatgccgat gcagcgcgta aatacggtga atcgctgttg gcagccgaaa
       61 tacccaaaga ggaaaaacta gaagttcttg cagcacgaaa taatgctggg gaatctgctt
      121 tgttcatagc tcttcaagaa ggtcattccg ctgcgattca agcttatgga gattttatta
      181 aaacttttga tttatcacca aaagaaacga ttaaactatt ggatgtaaga gataatgagg
      241 ggttaccagg attatttctg gccgcaggga aagggaatat cgaggctatg atggcatata
      301 taaatatatg ccatcatagt gggataaaac ttacagaaat agcagacaga cttaacaata
      361 atgaacaaga catgtttaat attatttctg acaaaataca agagttgttt taagtgtgct
      421 aaatagctgc aaagaattgc acttagaagg atctctcccc gaattgtgtg atctgcttaa
      481 tttctaaaaa gaaacaaaca gttaactaga ctgagcgata ctacccatag aaccagtttc
      541 ccatgacgaa cgacactgga cgcaaaaaca atcataaaaa acacaataaa tgtaatgaac
      601 acatcccacc ctattaagta aagtgcactg tgatagagga agggaaatac aggaaggtgt
      661 ggagccttgc agatttctgg catcatagtg cccaatacgg aatgcataaa gccccctcag
      721 actggagact ccacaaatga aatatacagt gtttaattat atcaagtgcg attacaatcg
      781 ctgacgacgg cacagttggt gtggaggtct cagtcctgaa caatttgaaa accagaacct
      841 cacttaagcc tgtgttcata ttacgtgggt aggatcatac ttttatagaa gctgtcagcg
      901 gctacgtccc agccaaatca cttcatgctc cagcgccaga gaggcaaggt tgggtttgct
      961 cagctgccgg ttgattttgt tgcgattgaa ccggacagcg tccagggcgt cggcaaaaga
     1021 gcgaatctca ccaatcgttg tttcattatc cgcattaacg atagcttcaa gaaacgccaa
     1081 ggttttatcg aattcatcag caacagtggc tccgggcata ccgtgaccgt ttatacaaaa
     1141 cggcgttttc agcgtggagt tttcatgaat agcctcaaca cgaatgtcgt gaagccagtg
     1201 atgtatcggc tacgatcctc agctgatgcc agaccatagg actgacacta cgaacagcaa
     1261 ttttaatgac gtaaatcttc ataactacaa ctaacagggc atcattatga atgatgccgg
     1321 ttattttacc gctgttggtc ccttcattta gggactgccc caagtttttt catactctct
     1381 ttctcagaga gtttagtctc tgggattcca gacggtccat cattgagcca agggagtaaa
     1441 gaactcaaag catttacaga attgtttgaa tcagctaaaa taatgacaat gttactattt
     1501 tacatgacaa agtgattata atgcttggaa catcttttaa taattttgga atcagtttat
     1561 ctcacaaaag atatttttca ggaaaggtgg atgagattat ccgctgtaca atggggaaaa
     1621 ggatagtaaa aatatcatca accaaaataa atactagtat tttgagctct gtttcagaac
     1681 aaatcggtga aaatattaca gattggaaaa atgatgaaaa aaaagtctac gtatcccggg
     1741 tggtgaacca atgtattgat aagttttgtg cagaacacag ccgaaaaatc ggcgataatc
     1801 tccgtaaaca gatatttaaa caggttgaga aagattatcg catttctcta gacatcaatg
     1861 ctgcacaaag ttccataaat catcttgtat caggaagcag ttatttcaaa aagaaaatgg
     1921 acgaactttg tgaagggatg aatagaagcg ttaaaaatga tacaacaagc aatgttgcaa
     1981 atcttatttc cgatcagttt tttgagaaaa atgtgcaata tattgatcta aagaaattac
     2041 gtgggaatat gagtgactat attacaaatt tagaatcgcc tttctgatga cagaacatcc
     2101 cgagtaaaga aactaagaaa agtaaatacc caggaattgc ctgagcggtg ctatatttct
     2161 cctcgcaagt aagtcctaat atattgaatt gttgtatagc cagtttttgt attttcctag
     2221 ctcatggatg tgggatccct ctcccctttc tctgctcaaa ggtaagcggc tcgtcagaat
     2281 catattgata tttactgaga agtcagatca actttccaag gcaacagatc gcgtacccgg
     2341 tttgccggtc agtcctggat atgttcaata acgtaacgca gccatttttc tggctccaca
     2401 ttgttcagac ggcatgtgcc gatcagcgag aacaacaccg ctgcatgttc gtcaccgctg
     2461 tcggaacctg cgagtcgagt cagccggaga gagtttcact ctccggctgc ttacggaacc
     2521 gtacgtgaca ctctcgcgtc atacggctct tgatcactca ttcgatgcac gtagtaaata
     2581 ccagtgagca aattgatctt acccagcaat acggtcagtt accgcgcaca tggcctgcct
     2641 gtgtcagaaa atctgctgga gcaggatttt tacgccagtg gtccgaacca gaagtgggcg
     2701 ggagacatca cgtacttacg tacagatgaa ggctggctgt atctggctgt ggtcattgac
     2761 ctgtggtcac gtgccgttat tggctggtcc atgtcaccac gcctgacggc gcaactggcc
     2821 tgcgatgccc tgcagatggc gctgtggcgg cgtaagagac cccggaacgt tatcgttcac
     2881 tcggaccgag gaagtcagta ctgttcagca gattatcagg cgctgctgaa gtggcataat
     2941 ctgcgtggca gtatgagcgc aaagggctgc tgctacgata atgcctgcgt ggaaagcttc
     3001 tttcattcgc tgaaagtgga atgtctccac ggagaacact ttatcagccg ggaaataatg
     3061 cgggcaacag tgtttaatta tatcgaatgt gattacaatc gctggcgacg gcacagttgg
     3121 tgtggcggcc tcagtccgga acaatttgaa aaccagaacc tcgcttaggc ctgtgtccat
     3181 attacgtggg taggatcact tacgccctgt ggttgaaacg tgagcatatt gttttcagat
     3241 gctgttgtct tgcttcagag tgatctgtcc tccggattta cgcaaagtta tccacaggca
     3301 ctgtgcattt aaaaaactta tcttttcttc ttgattttaa aaactcagat cgtcatatca
     3361 ttatgataaa cataataaaa aactctaaac atgagaaggt ttttaacgta aggtgagagg
     3421 atttttaagt gtatccatag attaaaaatg acaatgcctc caattagttg aattaggggt
     3481 tataataccc gcttttaagg ttctttcatg gtcacagtta actttcattg cccccgttgc
     3541 cagtccgttc aggtttaccg tcacgggcag aacccaaaag gacatgatcg attccgttgc
     3601 cgtgactgtc accgggtgtt tcagctcact tactgttacg aggcccgtaa accgggagtt
     3661 aaaaaccaaa tcactgaaat ggctttcaac ggtgccggag ttcgcgatac tgcccgtacg
     3721 ctgaaaatcg gcatcaatac cgtcatacgg acattaaaaa gctcccgccc cggcggataa
     3781 catctgcgcc agtcgctcac gctgatgttg cccttatctg tgaacttgat gggcagtgga
     3841 gcttcgttgg cagcaaggcg caacaacact gtca
//
LOCUS       DADMGC010000227         3844 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.228, whole genome shotgun sequence.
ACCESSION   DADMGC010000227 DADMGC010000000
VERSION     DADMGC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.228"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            1058..1498
                     /locus_tag="JNZ73_004081"
     CDS             1058..1498
                     /locus_tag="JNZ73_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734140.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(1577..3274)
                     /locus_tag="JNZ73_004082"
     CDS             complement(1577..3274)
                     /locus_tag="JNZ73_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HAY8734141.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(3594..>3844)
                     /locus_tag="JNZ73_004083"
     CDS             complement(3594..>3844)
                     /locus_tag="JNZ73_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="phosphotransferaese subunit; phosphorylates
                     dihydroxyacetone along with DhaK/DhaL; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAY8734142.1"
                     /translation="PQFNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALI
                     ARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         1005 a          854 c          914 g         1071 t
ORIGIN      
        1 tcctgaaaag caaaatcgat atatactttc atatatatcg ataaaaatca tgttcaatat
       61 caaagtttag ctattgaaaa catgaagatg tgacgacttc cgcaagcctg ttgaccctct
      121 ctatcattaa aaatgtattt atcaggtggc taatattatt taaatcattt aaggctaaat
      181 catttaaata tgaatgacat tattgttttt tatcgtccac ggttacgcga cgttgaagtt
      241 acttgtttta caatgagcga tttttgatat ctcgccgaga aaattatcag cattctctcg
      301 tttcagtgtc gtgaatatac tgttaacgga ggtgaaaaga atgctggggg ttatacaaaa
      361 cctatgttgt tgtcgtccgg attcaccgct tgctgatagt aagcagcgta accagaagtt
      421 accgcaacaa gactttgagg attttaagaa cgacgttatt aatgtcatta acaacaatga
      481 agatatttat tttcttagag agagtaattt actgaatgcg tttattcgaa cgtgcaaaaa
      541 aagagatgat aacagttacg gaaatatgat atttacgctt tattttgata acgatatcta
      601 tgggaacaga attatcaaag catcgctgaa cggcggagag ttgcagttat taagcacagg
      661 gtgtagcaat gataacattc tccattttat taatgcattt ctcattcggg tgaaatttcc
      721 gctacctgga cgcataaaac cggaactctc gccaaaagcc gggcactccg aaggaattga
      781 cgcgttgcgc tgctctgtaa cggcatttac cgcaagccat ttatttcgcc agcgacaatc
      841 cgcagagaac caaacgcatc aggaaacgac tgaacacaat aaatactgta ttgatcaaca
      901 tattatactg gcacctccag cacgcagtga ggatacactg tatcatcagc gaatcttatc
      961 actgcggagc tggttgaacg actttctgag cgaaataagt cggttcccgg taacaacaac
     1021 agaatccttt tttaattatt gtttcgttgt ttttggtgtg attcagaaaa taaatatttt
     1081 agacagtagc cagtcaggcg ttaaaaatgg ggcggatagt gaggcggttt tggctgcgct
     1141 tattgaacat ttagaattga tcaatccttc tggcagatta agccaaaata cccgttcggc
     1201 aatgcttcag ttacgcgaag aggaatggtc tgaattcttt ttttggttgc taaattctct
     1261 ggagtgtctg gattatgtga ttattaattt aactccagaa agcaaaaaaa cgttgatgag
     1321 tgaacaccgt aacaatattc aggtagcaat tgatgcttta tatagccaac gtcggcgtaa
     1381 atcgccgggt gatgaaagtg aaacacttac ccgtagaaat gacgctatct tcggcaacca
     1441 cgtatggcaa acatttgcgc aatacttccc gccaggactt gaaaaaccgt ctgtgtagaa
     1501 aaaaccatag atatggtgtt atctgccgac aacgccatcc ggagaagccg aatgacgttg
     1561 tcggagtaag cgctggttaa ggtgtgggtt gtgcctcttt ggttgagggt tgcgtcgttg
     1621 ctgacttaac ggtcggacgc gtcgccggaa cattgtcaca cggttgctct ttcgggcaga
     1681 tcaaatccag cattttcagc gtcacgccat tggtccagcc aaagccatcc tgtaatggat
     1741 attcgccacc gccgcccccc gttccggtgg tgctgacatc atatttttcc accagctttt
     1801 tctcccggtc ataggtgtgc tgaacattgg tcaggaagtg ccagctaatg tccatcgcca
     1861 cctctttttg cccgtagttt tgtaatcctt ctgtcgcgac ccactgtaac ggtgcccagc
     1921 catttggcgc atcccattgt tgcccacttt tcaccgacgt ggtgttcagg ccgccgggtt
     1981 gcagcagatg tgttttcgtc gccgtcgcca ttttgttggc gcgatctttc gctgccgcat
     2041 tgacgtacag cgggaacagg gcggccgcgg ttaactgatt gcgcacttta tgacttttca
     2101 ggtcgtaatc ggcataccag ccttgttgat cgttccacag gtatttttcg atcccttttt
     2161 gacgggcatt cgccagcgtt tcgtactggt ttgccatcgc gttatctcct gcagctttgc
     2221 tggcgcgggc gaggattttt tccattttaa acatcaggct gttcagatcg accggtacga
     2281 tgctggtggt gcgtaaggta tttaactgct gcgggttgtc catccagcgc gagctgaaat
     2341 cccagccaga cgcagcggca gagcgcaggt cgcggtaaat ttcagtggca ggtcgattcg
     2401 gattgctttt ggcggtggca atatcttcca cccatgactc tggtcgtggc gtatcgcgat
     2461 cgtcccagta gcggttgaga agggtaccat cctgaagttt tacaacgcgt ttttcctgtt
     2521 gtccggcttg caggttttca acaccgtcca tccagtaagc atattctttt tgcatttgcg
     2581 gcaggtattg cttcaacgcg gcatcgcctt catgctgcgc cagtaactct accatcaggg
     2641 caaagaaggg cggttgcgag cggcttaaat agtaactgcg gttgccgttg ggaatatgac
     2701 cgtaagtgtc tatttcatga gcaaaattgg ccaccatatc cgcgacttta tcccagtgac
     2761 cgctttcggc aagtcctaac atggtgaagt aactgtccca gtaatatacc tcgcgaaaac
     2821 gtccgcccgg cacgacataa ggttccggca gcggtaacag agaatcccat ttttcggtgt
     2881 tttcggtaga acgcgttaat accggccaaa gtccgtcaat atgttcgcgc agtgactgcc
     2941 cctctggcgg aacatatttc tcgccttctt tcggcagggt gaaattgacg ttaacgaaat
     3001 ggcgcagatc aaatccgctc tggttttgct gcatccgata atcagcaagg atcatcagcg
     3061 gatcgctgtt cggcacggca tcggcaaagg ttttttggtc cggaaaaagt ttggcgtttt
     3121 gcacatcatt aaacagcggc cctaataaaa tatcaggcgg ctgtggtgtt accggtgttt
     3181 cttctgcctg caccgatagc gcagcgaaac acaaaaagat acaggctgga attaacgcca
     3241 ttttttgcgg gcgagaaggt gcgggggatt tcatcaatca ttctcctttg gcgaaaccga
     3301 ataaaacggt attcaagtat cagaaaacct tagttcagga tcgcactagc tgcatgatcg
     3361 aaatctcatt ctgcgaacgg tcaggcattt tagaaaaggg aattaaaggg ataaataacg
     3421 gcgaaataga gtgatcaaat aacgtctgtt tattagccag ccagcgacat ttggttcgcg
     3481 attagcgaag aaaaatcatc ataaattggt aaaaaatata acaggtagtt aaaaaacatc
     3541 agtgctgggt aaattgccgg atgacatcag aacgatgcca tccgaacggt ggcttaaccc
     3601 tgacggttga aacgttgcgt tttaacgtcc agcgttagcg tttcttctgg ttgtatcgca
     3661 tacagtttct caccctgctg gcaaatccag ccaatcccca gttcacgggc gattagggcg
     3721 ctgtgggata ccggacttcc ggcgctaagg cagatacctt ttacaaccgc cggatccagt
     3781 tgcagtactg tggaaggata aatgttctcc gccagtagaa tagttggcga gttaaactgc
     3841 ggga
//
LOCUS       DADMGC010000228         3792 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.229, whole genome shotgun sequence.
ACCESSION   DADMGC010000228 DADMGC010000000
VERSION     DADMGC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.229"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            162..2159
                     /locus_tag="JNZ73_004084"
     CDS             162..2159
                     /locus_tag="JNZ73_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HAY8734143.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2222..3499
                     /locus_tag="JNZ73_004085"
     CDS             2222..3499
                     /locus_tag="JNZ73_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HAY8734144.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          924 a          865 c          950 g         1053 t
ORIGIN      
        1 ctgcttaaaa aaagaacaaa tggtagtgtc cgctattgcc agtacacctc aacattccac
       61 catgcattac gattaacgcc gcatagccag ttgaactttg ctactttgtg agaggtagta
      121 ccttctatcc agtgcgaatt taattaatgg aataaatgat tatgagtgaa aatgatacaa
      181 tcccaaagaa gtctacaagt cagattaaca aagcggtatt ctttacatct gctttgctaa
      241 ttttccttct tgtcgccttt gccgccgtat tcccggatgt cgccgacaaa aattttaaac
      301 tacttcagca acaaatcttc acgaatgcca gctggttcta catccttgct gtggccctga
      361 ttttactgag tgtcacgttc cttggactct cacgctacgg tgatatcaag ctgggcccgg
      421 accatgcgca gcctgatttc agctaccact cctggtttgc gatgcttttt tcggcaggga
      481 tggggatcgg cctgatgttc tttggcgttg ccgaacctgt aatgcattat ctttcgccac
      541 ccgttggcac tccagaaacc gttgcggcag ctaaggaagc aatgcgtctg acctttttcc
      601 actggggact gcacgcatgg gcaatttatg ccattgtggc gctgattctg gcgttcttca
      661 gttaccgtca cggtctgcct ttaactctgc gctccgcact ctatcccatt attggcgatc
      721 gcatatacgg acctgtagga catgcggttg atattttcgc tgttataggc acggtctttg
      781 gcgttgcgac atcactgggt tacggtgttt tgcaggtgaa tgccggtttg aaccatcttt
      841 tcggggtgcc catcaatgaa acggtgcagg taattctgat cgtggtcatc acggggttag
      901 cgacgatttc agtggtgtcc ggtctggata agggaatacg tatcctgtct gaactcaatc
      961 tgggtctggc tttgttgctc ctggcgctgg tcctgtgtct gggaccaacc gtgcttctgc
     1021 tgaagtcatt tgtggaaaat acgggcggtt atctttcgga actggtgagt aaaacgttca
     1081 acctttacgc gtatgagccc aagtcgagca actggctggg gggctggaca ttactgtact
     1141 ggggatggtg gctttcatgg tcgccgtttg tggggatgtt catcgcacgg gtctcccgcg
     1201 ggcgaaccat tcgcgagttt gtcaccggcg tgctgtttgt tcccgcgggt tttacgctaa
     1261 tgtggatgac ggtgtttggt aacagcgcga tctatctcat tatgaaccag ggggccacag
     1321 acctcgccaa tactgttcag caggatgtgg cgctggccct gtttaatttc ctggagcatt
     1381 tcccgttctc ttctgtgctg tcattcattg caatggcgat ggtcatcgtc ttctttgtaa
     1441 cgtctgctga ttcgggggca atggttgtgg atactctggc atcaggtgga gtggcaaaca
     1501 cacccgtctg gcagcgaata ttctgggcct cgctcatggg cattgttgca attgcgcttc
     1561 tccttgccgg agggctaagt gcgctgcaaa cggtgacaat agcgagtgca ttgcccttct
     1621 cagtgatctt actaatatct atatacggac ttttaaaagc tttgcgccgg gatttgacca
     1681 agcgtgaaag cctgagcatg gcgacaattg ctcctacggc tgcacgtaac ccaattcctt
     1741 ggcagagaag gttacgcaat atcgcgtatc tgccgaagcg atctcttgtg aaacgtttta
     1801 tggacgacgt tatccagcct gccatgacgc tggttcagga ggaactgaac aagcagggga
     1861 cgataagcca cattagtgat gcagtcgacg atcgtattcg tcttgaagtc gatttgggca
     1921 acgagctgaa ttttatatat gaagtgaggc ttcgcgggta tatctcaccg accttcgcgc
     1981 tcgccgcaat ggataatgat gagcagcaga ctgaacaaca tcgatattat cgcgctgagg
     2041 tttatctcaa agaaggcggt caaaattatg atgtgatggg ctggaaccag gaacagctga
     2101 ttaatgacat actggaccag tacgaaaaac acctgcactt cctgcacctg gttcgttaat
     2161 agcaacatgc cgtcctgggg gcggcaatta ttatcccggc cgcaatatga gggaatgcag
     2221 aatgatttca cgctggaaat ggatgctgaa gcagacaatt aaaaaactat ggttcagggc
     2281 aacgttattc gcaattgtcg cgataataac ggccctttta tcaattcttt ttaaatcaat
     2341 gatacctgag tcggtttccg tgaaggttgg tgcggaagca gtcgataaca ttctgaacat
     2401 actggcatcg agtatgctgg cagtgaccac attttcgctg agtatcatgg tcacagccta
     2461 cggttcagcc actactaatg tgactcccag agctacgcgt ttagttgttg aagacgtcac
     2521 cacacaaaat gtactggcca ccttcatcgg ttcttttctc ttcagtctgg tagggattat
     2581 tgccctcaat atgggagctt atggagaaag ggggagagtc attttattca ttgtcacact
     2641 ggttgtcatt gccttaatcc tcatcacatt gcttcgctgg atacagcatt tgacctctct
     2701 ggggagggtt ggtgagacaa cggcacaagt agaacaggcg gccatcgaaa catttattgc
     2761 gagagcaaga aatccctgtc tcggtggata tccatggctt gagaacaatg aacagccgaa
     2821 aggaacggtt gcagtttatc cgaagaagat tggctatgtt gaatatattg atatggtgaa
     2881 acttagcaag ctgctgacca atgatccccg tcatgtatac ctcgtggcgc agccaggcag
     2941 tttcatacat ccgtccatgc cagttttgta cctgagtcag ggccaggagt cgtcaatcaa
     3001 caccgattta cttgagacga ttattgtctc ggatgcacgt tcatttgctc aggatcctcg
     3061 attttgtctt agcgtcatgg ccgaaatagc ctgccgggcc ctttcccctg cagtgaacga
     3121 tcctggaacc gccattgatg tcattggcag aggtgttcgt atactttcca cttacgcgca
     3181 gaataaatct gatgaaatag aagtgaaata tccttcagta catgttgcac cacttcagaa
     3241 taacgatcta ctggaagact ttttctcacc tgtcgcgcgc gatggtgcca gtatgaggga
     3301 gattcagata agagtcctta aaggactgtc gatgctgagt aaaggttggc ctgagatatt
     3361 tgctgaagcc gcacataccc tggcatttga aacattagag catgcaactc gtgctgacca
     3421 tatagattct gatagatatc taataaaatc aacttattat aatttattta gtggcgaata
     3481 ttctaataaa aaaacatagc tgcagagcaa gcaattcggt tgttggcaag tcaagggcaa
     3541 agcattcgcg tgagaatggg cgtatgcttt gtgccaaaag caaacgttgc taattctgca
     3601 ctgttcagcg atggcgcagt agagacaggc actgccactg tccagtcggc aaaagcgaac
     3661 actttgccac tgcggggata aactgattga agctctcgcg caaaatcact tattcttttg
     3721 cgttaataaa atgtaggaga tgggtcattg atcctaccca cgtaatatgg acacaggcct
     3781 aagcgaggtt ct
//
LOCUS       DADMGC010000229         3789 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.230, whole genome shotgun sequence.
ACCESSION   DADMGC010000229 DADMGC010000000
VERSION     DADMGC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.230"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..222)
                     /gene="ospD1"
                     /locus_tag="JNZ73_004086"
     CDS             complement(<1..222)
                     /gene="ospD1"
                     /locus_tag="JNZ73_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspD1"
                     /protein_id="HAY8734145.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPT"
     gene            complement(593..730)
                     /locus_tag="JNZ73_004087"
                     /pseudo
     CDS             complement(593..730)
                     /locus_tag="JNZ73_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(812..1110)
                     /locus_tag="JNZ73_004088"
                     /pseudo
     CDS             complement(812..1110)
                     /locus_tag="JNZ73_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1699..1858)
                     /locus_tag="JNZ73_004089"
                     /pseudo
     CDS             complement(1699..1858)
                     /locus_tag="JNZ73_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1862..3091)
                     /locus_tag="JNZ73_004090"
                     /pseudo
     CDS             complement(1862..3091)
                     /locus_tag="JNZ73_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(3111..3458)
                     /gene="tnpB"
                     /locus_tag="JNZ73_004091"
     CDS             complement(3111..3458)
                     /gene="tnpB"
                     /locus_tag="JNZ73_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY8734146.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(3458..>3789)
                     /locus_tag="JNZ73_004092"
     CDS             complement(3458..>3789)
                     /locus_tag="JNZ73_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8734147.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQILIR
                     EIKGSSH"
BASE COUNT          931 a          918 c          868 g         1072 t
ORIGIN      
        1 tgtcggaatg gatatgctct gtattttggc caattttctg aaagaaacac cactatatcc
       61 cccccctgat ttttcttcat tgatggcgtg ggatatagcg gtatttgtca cgttaatgat
      121 attatttttc attcctttat tttcattagc agtattgctg cctattatta ggtgcatatt
      181 tttgttgttt gctggatgta atccatagtt atttattgac atttttgttc atcctttttg
      241 ctaaaaaaaa caataaccta tttttgtaat gtaatagata ttagtctatt gctaatctaa
      301 aaaaaccatt gccacattga atggctatat tcaccaataa attctttcga tttcgtatat
      361 tgacatatcg tagatatatc ttatccctaa gattcaataa tcgagtccgt ttaccattct
      421 gtgcaagcgc cttcactcag aagtgaccgt caaggcgatc accaaacttg ataataaagc
      481 agctcattgc catacgccag tccttaaggg gcgttgtcca tttctgtgat gctgcctaga
      541 tttaactgcc agctccggct tatctacatc taaatatgtg agcctaatac cgctacactt
      601 tgtccagact atgtttttct ccgggaagtg ggctaaccgt ctccataaag tgacgaactt
      661 ttttcaacag tggccatatt gaacggcact gatgattacg ggttattgtc tcatgaagag
      721 ccggccacat cactgacagc agtgacttca ggccttcagc accagacagc ctgaaccagt
      781 taatccatcg cccgacagat ttagtaatct agggctggtt tttcttcagc tgttgcggtt
      841 cgaaagtgcc attacgatca cgcagagtac ggatttccag aggttcatct ccagtagtga
      901 taatcttcgt taagcaacca ttgcgggaat tgctgccaga tctaagctga tttttctcgc
      961 acccaagatg atgagtcata ttagcattga gtacggtttc attttcagca tccaatcaaa
     1021 ctgactgaga tcttcaggat ttttgagact tttggctagt tcattagcta aagcccgcaa
     1081 cttattagat gtcaactgtt tttcattcat aaattcacct tcatttgata tcaaattaaa
     1141 tagttcaaca tcagagaatt atacaaactt atgtacaggc tcctatgatc ttcgaatcct
     1201 gaagattata gtgaaaataa gttttcacgt tgaaatccca catagttagt atatgataaa
     1261 taatcatgga tgacctgatt aacctcttgg ggtagtgctt ttatagaaga ataaatattc
     1321 tactaattta atacatcacc taaaatagta ttgtttttta tggttggttg aaggtcgtgg
     1381 tgatgatgtt agtccttttg agaatattcc agtgccagat cttacttata ataaaggggc
     1441 ttggcagttt ggataaacta ttgttcttat tccactaata caaacttttt gtaaagtata
     1501 ttaaatatac ttcatttcat actgaaatcc ccggaagtat tcaatcaaac aattacacca
     1561 taaaaggaag agggggccct aattaagttg tcaagcatgt ttatgatacc tgcagggtat
     1621 aaattttctg gctgcaagca gacacatata aaacgtcttt attgatccta cccacataat
     1681 atggacacag tcgtaagtga ggctctggtt ttcaaattgt tccggactga gaccaccaca
     1741 ccaacggtgc cgtcgccagc gattgtaatc gcactcgata taactaaaca ctgttgcccg
     1801 cactatttcc cgatggataa tagtgttctc cgtggagaca ttccattttc agcgaatgaa
     1861 ttttcatctg ttgctgtatc cagtcataca gtgactgcat cagtggcgcg gctctggctt
     1921 ttcttgccgc cagacgctgt tctgctgaac atccccggac ttctgcctcg atggcataca
     1981 gttcaccgat acgctgcagg gcttccgtgg tgatgtcggt tggcactctt gcatgcacat
     2041 cgtggatttt tctccggaca tgagccatac atgcggcttc cgttattctg ccggattcgt
     2101 acaacgcccg gtaaccaccg taagcatcgg cctgaagcac tccgctgtaa ccggccagat
     2161 gattctgtgg atggatacct ttccggtccg gtgagtacgc gaaccagacc gccggaggca
     2221 tttccgaacc ggcgttacgg tcatcacgaa cgtagaccca cagccgggca gtccttgttt
     2281 taccgctgcc cggctcctgg accggaacgg ggatatcatc ggcatggact ttaccgggca
     2341 tcagcacata ctggcgcagg atgtcataca gcggttccag cagttcagca acagcacctg
     2401 tccagcgccc cagtgtggca cggctcagct ccacgccctg acgacggtat atttctgact
     2461 ggcggtataa cggcagatag tctgcatatt ttccagcgac aacatgggcc agaagccccg
     2521 ctccggcata actgcgtgca atgggttttg aaggcaccgg tgcctgcacg atatggtcac
     2581 accggcaaca ggccagtttc ggacgttgtg tttcgataac cttaaaggcg ctgctgataa
     2641 gctccagctg ctctgacaca tcacatccca gagaactgag ttcgccacca caggccgggc
     2701 agcattcctc ttccggacgg atgacccggg tttcacgggg aagtgaggca ggtaacggtt
     2761 tacgggctga agactggcgc aggggagatg gcagtatcgg gtcatattgc tcacccagcg
     2821 tttccgccat ttcttcctga agtgcgctga ttcgctcctg tgcttccagt atctgccgtt
     2881 cggtttttgc gcgaagtttt tctgagcttt taccgaactg catgcgctgc agtttcgcaa
     2941 ccagcgcctt cagccggttg atttcggaag cataagccgc cacccgctgt gagagcaggc
     3001 ggttgtattt cgccatctgg cggatggtgt cctgttgcgt ctgcaacagt gcccgcaggc
     3061 gggcattctc atgagaaagt gaggtgtcca tatcctcact ttacaacggg ttatatgcgg
     3121 atgccagcgc gttccgttcg tttcgggtgt ttccagtcga tgccctcaag aagcatggat
     3181 aactgggccg gagtaaggtg caccttgccg tcacgggtga ccggccagac gaagcggccc
     3241 cgctccaggc gtctggtaaa gaggcacagt ccgtcactgt cagcccacaa cacttttatc
     3301 tggtcacccc ggcgtccgcg gaagatgaac agatgcccgg agaacgggtc atccttcagg
     3361 acgttctgaa cttttgaggc caggccgtta aagccatttc gcatatcggt gatacctgca
     3421 accagccaga tacgcgaacc tgcagggaaa gatatcatca gtggctgctc ccttttattt
     3481 cgcggataag tatctgtaat aacgccggcg tcagtttacc tttaagcctg agagttccgg
     3541 ccggcagaac cagctcacag cacagactgt cggacggtgt atttatctgc tccggcgtta
     3601 acgtcacggg aagcagtgca ggcatatttt ttccggaagg cagcaggcca cctttccggt
     3661 attgatggcg ccagttgaag agcaggttat cgttgattcc gttttcacgg gcgatctgcg
     3721 ccacacaggc tccgggctgc agtgactgct ccactaaagc gattttaaat tcataaggaa
     3781 aattaggcc
//
LOCUS       DADMGC010000230         3736 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.231, whole genome shotgun sequence.
ACCESSION   DADMGC010000230 DADMGC010000000
VERSION     DADMGC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.231"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            190..447
                     /locus_tag="JNZ73_004093"
                     /pseudo
     CDS             190..447
                     /locus_tag="JNZ73_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="JNZ73_004094"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="JNZ73_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HAY8734148.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHKQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /locus_tag="JNZ73_004095"
     CDS             complement(1599..2351)
                     /locus_tag="JNZ73_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HAY8734149.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="JNZ73_004096"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="JNZ73_004096"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAY8734150.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="JNZ73_004097"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="JNZ73_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3605..>3736)
                     /locus_tag="JNZ73_004098"
     CDS             complement(3605..>3736)
                     /locus_tag="JNZ73_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8734151.1"
                     /translation="AHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
BASE COUNT          965 a          886 c          889 g          996 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagatc atcaatagtc agggaaggaa gttgcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt tcaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgcttgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatga
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa atacccggac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttagcgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aatcatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggc
//
LOCUS       DADMGC010000231         3686 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.232, whole genome shotgun sequence.
ACCESSION   DADMGC010000231 DADMGC010000000
VERSION     DADMGC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.232"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..487)
                     /locus_tag="JNZ73_004099"
     CDS             complement(<1..487)
                     /locus_tag="JNZ73_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="required for cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY8734152.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LL"
     gene            760..948
                     /locus_tag="JNZ73_004100"
     CDS             760..948
                     /locus_tag="JNZ73_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734153.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            960..1670
                     /gene="bcsQ"
                     /locus_tag="JNZ73_004101"
     CDS             960..1670
                     /gene="bcsQ"
                     /locus_tag="JNZ73_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HAY8734154.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAEEILTLA
                     NWCLLNYSGLKTPVGSAS"
     gene            1667..3686
                     /gene="bcsA"
                     /locus_tag="JNZ73_004102"
                     /pseudo
     CDS             1667..3686
                     /gene="bcsA"
                     /locus_tag="JNZ73_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
BASE COUNT          853 a          922 c          970 g          941 t
ORIGIN      
        1 caagcaacaa tgaaaattgt ttatcgttat tatttccggg attaattacc aaaagcgaac
       61 aatggtttaa cctgctccat ttattcattt tatccaacca tgagcgaagc cgctcggcag
      121 gaatgttttg ccatgcgtta tttgcgcaaa caagaataaa aaggtaatta tggggatcaa
      181 tagaacactg caaatcacgg gccaaatagt atagaccttt ttcatgattt agcattgaaa
      241 ataattttat tttttccggc ccttgagaat catctaattg aaagattttc gccggatcgc
      301 tgtccatgct aatgaccgcg acgtgtgcgg tttcagcctg ggatgcaatt gtttgattcg
      361 ccagactgat agcatcttca tggcgatcga cgttaaacca ccagacgccg cctgctggca
      421 tatgtcgcag ctcatcccat aatgatgaga taccgataga gaatacaggg tccacaatgt
      481 ccctcatgtc accattttgt ctgattatca gtttactagc gaaagctaag aaataaacct
      541 accattgaaa ttaaaactta tcagatttag catgtgaatc aatttagaag gtcacaagac
      601 gtgtgatttt agtcttttcg tttttaactt tttctgttgc gtaacggaaa gtcaaaaagt
      661 gagcaaattc ccgtctcgcc gtaaacaata aaccggcgaa atgctcacgg ttagaattgt
      721 atcatgaatg gtacggttat ttcataggga tcaagcaaaa tgaataacaa tgaaccagat
      781 actctgcctg atcccgcgat aggctatatc ttccagaatg atattgtggc gttaaagcag
      841 gcattttcac tgcctgatat tgattatgcc gatatttccc aacgcgaaca gttggccgcg
      901 gcattaaaac gctggccgtt gctggcagag tttgcgcaac aaaagtaggg gattggtgaa
      961 tggccgtact gggattgcag ggggtgcggg gaggcgtggg gacaacaacc atcaccgccg
     1021 cattagcctg gtcattacaa atgttgggag aaaatgtcct agtggtcgat gcctgcccgg
     1081 acaacttgtt gcgcctgtca tttaatgttg attttaccca ccgtcagggc tgggccagag
     1141 cgatgctgga tggccaggac tggcgtgacg ctgggttgcg ctacacctcg cagctcgatt
     1201 tgctgccttt tggtcagtta tccattgaag aacaagaaaa tccacagcac tggcaaaccc
     1261 ggctgagcga tatttgctcc ggcttacagc aactaaaagc cagcgggcgt taccagtgga
     1321 ttttaatcga cttaccgcgt gatgcctcgc agataaccca ccagctgctg agtttgtgcg
     1381 agcactcgct ggcaatcgtc aatgtggatg ccaactgcca tatccgactg catcagcaag
     1441 cgctgccgga tggcgcacat attttgatta atgacttccg tattggcagt caggttcagg
     1501 acgatattta ccagctttgg ttgcaaagcc agcgccgatt actgccgatg ctcattcatc
     1561 gtgatgaagc gatggctgaa tgcctggcgg ctgaagagat actgacgctg gcgaactggt
     1621 gcctgttgaa ctactccggg ctgaaaacgc cagtcgggag tgcatcatga gtatcctgac
     1681 ccggtggttg cttatcccgc cggtcaacgc gcggcttatc gggcgttatc gcgattatcg
     1741 tcgtcacggt gcgtcggctt tcagcgcgac gctcggctgt ttctggatga tcctggcctg
     1801 gatttttatt ccgctggagc acccgcgctg gcaacgtatt cgcgcagaac ataaaaacct
     1861 gtatacgcca acgcctcgcg tccgcgtccg ctggacccgg tccgttatct cattcaaaca
     1921 tgctggttat tgatcggtgc atcgcgcaaa gaaacgccga aaccgcgcag cgggcatttt
     1981 caggtctgca aaatattcgt ggacgttacc atcaatggat gaacgagctg cctgagcgcg
     2041 ttagccataa aacacagcat ctggatgaaa aaaaagagct cggtcatttg agtgccgggg
     2101 cgcggcggtt gatcctcggt atcatcgtca ccttctcgct gattctggcg ttaatctgcg
     2161 ttactcagcc gtttaacccg ctggcgcagt ttatcttcct gatgctgctg tggggggtag
     2221 cgctgatcgt acggcggatg ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc
     2281 tgaccgtttc ttgccgttat atctggtggc gttacacctc tacgctgaac tgggacgatc
     2341 cggtcagcct ggtgtgcggg cttattctgc tcttcgctga aacgtacgcg tggattgtgc
     2401 tggtgctcgg ctacttccag gtagtatggc cgctgaatcg tcagccggtg ccattgccga
     2461 aggatatgtc gctgtggccg tcagtggata tctttgtccc gacttacaac gaagatctca
     2521 atgtggtgaa aaataccatt tacgcctcgc tgggtatcga ctggccgaaa gacaaactga
     2581 acatctggat cctcgatgac ggcggcaggg aagagtttcg ccagtttgcg caaaacgtgg
     2641 gggtgaaata tatcgcccgc accactcatg aacatgcgaa agcgggcaac atcaacaatg
     2701 cgctgaaata tgccaaaggc gagttcgtgt cgattttcga ctgcgaccac gtaccaacgc
     2761 gatcgttcct gcaaatgacc atgggctggt tcctgaaaga gaaacagctg gcgatgatgc
     2821 agactccaca ccacttcttc tcgccggacc cgtttgaacg caacctggga cgttttcgta
     2881 aaacgccgaa cgaaggcacg ctgttctatg gtctggtgca ggatggcaac gatatgtggg
     2941 acgccacttt cttctgcggt tcctgtgcgg tgattcgtcg taagccgctg gatgaaattg
     3001 gcggcattgc tgtcgaaacc gtgactgaag atgcgcatac ttctctgcgt ttgcaccgtc
     3061 gtggctatac ctccgtgtat atgcgtattc cgcaggcggc ggggctggcg accgaaagtc
     3121 tgtcggcgca tatcggtcag cgtattcgct gggcgcgcgg gatggtacaa atcttccgtc
     3181 tcgataaccc gctcaccggt aaaggactga agtttgctca gcggctgtgc tacgtcaacg
     3241 ccatgttcca cttcttgtcg ggcattccac ggctgatctt cctgactgcg ccgctggcgt
     3301 tcctgctgct tcatgcctac atcatctatg cgccagcgtt gatgatcgcc ctattcgtgc
     3361 tgccgcatat gatccatgcc agcctgacca actcgaagat ccagggcaaa tatcgccact
     3421 ctttctggag tgaaatctac gaaacggtgc tggcgtggta tatcgcacca ccgacgctgg
     3481 tggcgctgat taacccgcac aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg
     3541 aagaagagta cgtcgactgg gtgatctcgc ggccctacat cttccttgtt ctactcaacc
     3601 tggtgggcgt tgcggtaggc atctggcgct acttctatgg cccgccaacc gagatgctca
     3661 ccgtggtcgt cagtatggtg tgggtg
//
LOCUS       DADMGC010000232         3653 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.233, whole genome shotgun sequence.
ACCESSION   DADMGC010000232 DADMGC010000000
VERSION     DADMGC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.233"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(4..300)
                     /locus_tag="JNZ73_004103"
     CDS             complement(4..300)
                     /locus_tag="JNZ73_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HAY8734155.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            1052..1600
                     /locus_tag="JNZ73_004104"
     CDS             1052..1600
                     /locus_tag="JNZ73_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8734156.1"
                     /translation="MAVIGYCRVSTQDQTVDNQKLQIEKVHKVDKWFIDEAVSGATKA
                     TSRDGFRNMMNYVRCDDTLVVVAIDRLGRNTVDVLSNVEALQTKGVRVVSLREGFDLL
                     TPIGRAMLTMMAGLADLEKDLISERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQG
                     KTRLQIQEELGISRATYYRLAK"
BASE COUNT         1132 a          690 c          815 g         1016 t
ORIGIN      
        1 tactcagaac actttcttaa atggtttcac tgaaacgtgt tcatagactc ctgccgctac
       61 gtacgggtca gcatcggccc aggcctgagc tgcttccagc gattcaaatt cagcaataac
      121 ggttgagcca gtaaatcccg cagcccctgg atcgttactg tctaccgctg gcattggacc
      181 agctgtcaac aaacgacctt catcatgcag taactgtaaa cgtgctaaat gtgccggacg
      241 aacggaaagg cgtttttcga ggctatcagc tttgtcttga gcgtaaataa catacaacac
      301 gggcacaact ccttgttcgg gaaagttgta aagtacgtta tttgaaaggg cgaagatctg
      361 caacggaaag atgatgtctt tgttaaggtc atgcataaag taagggtaat tacgccaaaa
      421 atgacatttt cactgatcct gatcgtcttg ccttattgaa tatgattgct atttgcattt
      481 aaaatcgagg cctggttttt ctactgaaat gattatgact tcaataaccc ttgaatgaag
      541 tctattcggt tttctaggag acctgttaaa aacaggacaa tatcaggtga atgactatgc
      601 aatttaatag tagatttcca agtatctaag cagttttaca caaattctat gcagtatcgg
      661 tatagtctct ttttagcata acttattgaa atatatagag aatctgtgaa gtctcttttc
      721 agggaactat gcagggtgtt agtcgttcct ctctcgactc ctagaaaagt gctatataca
      781 ggactatata acgatatata gatgtttggt cacatggtgt aactatcaag aaacttaacg
      841 atttcctacg tgaaagttag aaagtgatgg ttatagttac cctcctaaaa tcttcatagc
      901 tccttcataa ccagtcttaa aaggacttcc ttttgagaca ctttcaacgt aacggtagaa
      961 tgcaccctca agtagtctca aaaggttgtt tttggattat gagacagcta aacttatgag
     1021 actaactttt gagacttcta aggggaacat tatggcggtg attggttatt gcagggtatc
     1081 aacacaagac cagactgtag acaaccagaa actacagata gagaaggttc acaaggtcga
     1141 taaatggttt attgatgagg ctgtctcagg agctacaaag gccaccagca gagatggttt
     1201 tagaaacatg atgaactatg taaggtgtga cgatacgtta gttgtggtag ctattgaccg
     1261 tttagggcgt aataccgttg atgtgctctc taacgtggag gcactacaga ctaaaggtgt
     1321 gagggttgtt tctctacgtg aaggttttga cttattaaca ccaatcggta gagcaatgct
     1381 tactatgatg gctggtttag ctgaccttga aaaagacctt atcagtgaac gtagaaaggc
     1441 tggtattgaa cgtgctaaag ctgaaggggt acatatgggg aggcctgtaa aagcttcttc
     1501 agaggctgta cagacgctta tttcacaagg taagacgagg ttgcagattc aggaagaatt
     1561 aggaatctct agagcaactt attacagact tgctaaataa gttactgaat agttacatgg
     1621 atgtagctga tatttggctt ttttgcaaag gggatcacat agctggtgta acaggtactt
     1681 agcagctaca gacatctgtc agatattttt gaagattctc agagagggaa tttgaagagg
     1741 ggctactatg attttataaa aattttcaga gtctgaaaag ttactgtaca gattttatac
     1801 aaactaaaaa ggactcccgt aggtgattca gtaatgaatt acttcacgaa gagtcccttt
     1861 acaagtcgat ttttaatcgt ggctacgaca atccacccat accagaccta accgtacccc
     1921 accttaccaa gccataccct accccgccag actataagga agcgtagaga accgatgaag
     1981 gtgtctgcac agttgcatct taaacaacca tcacagtgat gtcaaatagt tcttttaagt
     2041 aggaggggtt cccctatggt cacatatccc ctctctcccg cttagtgtcc cttgatagta
     2101 tctagagagt atgcctgtaa tgttagagag tatgcctgta atgtttctgt atcaggtcag
     2161 ctataagagg tctggatatt accttgtaag attcttggta gtgattatgc agagggcttg
     2221 ataagagtgt ttaacgggag gaagagaacc atgaaaccga aggtaggttc tatcttaaca
     2281 gtgactgtaa gagatacctg aaaaaatact ttaacagaac taccaagaag agagggaacg
     2341 gaagagaggg aacgataaga ccagaggtag tgacctgtta aacaggctgt ttcaaagtct
     2401 cttaaaagtc tgttaagagg gctaggtgaa caatcctgcc attttgtcaa gtgttttgtc
     2461 tttagcaaaa agtgctaaac ttaacaaaaa gcactaaatg ctaaagaggc gattaagaaa
     2521 tatactaaga ggggcgcttc gcttggggtc actatgttct ttctctcccg ttaagaggat
     2581 ttacagggac atttcaaagc cctgataagg taagcccgat gataacagaa acagagggca
     2641 aaaccactta tcaacactgt gaacgaataa cgtaaagggc tggttaagaa agtcggttta
     2701 gggattggta acaataccaa ttagaaggga gcttcccttt ctggggtgtc gctctatggg
     2761 tgaagttaac ctttgaagtt ctctgtgaag aaactgaaca gctattatat tggctaatta
     2821 caaccctttg aaagataagc atttttagaa gttactgtta aggttgcaga gttgtctgtg
     2881 aagagacttg tcagttctct atattgggtg gttacaactc attgaaaaat aagagctttt
     2941 agaggttact gtcagagagg tgaatacttg atagatacag tcttccggtt ctgtcaggct
     3001 attttttaac agatagcaca aaatgctaaa gatgccccta tgccatctcg tacagggggg
     3061 ttttgaaggg agtttaacgg gggtctgtgt ggtcattatc cttaaggtca agcgtccatg
     3121 tgcgaccgaa cagtttaaag acaacaagca ctctgacgag gacgtaacga agtagcagat
     3181 ctgcaataag tccaatacct ccaccagccc atacatcagg tgaccccagc tcaggaggga
     3241 gatttaccat tagttttcct tgtaaggcgc tctcaatcgc attctgacac aaaaaagtac
     3301 ctgaccttat gagttgacct gaagaggtca tttaactcgt tacagagcga ttgaagaggt
     3361 ctgtgtgcaa tgcaataacc atgtgaccag atacatagcc acgcctcata ttttactctc
     3421 cccataaacc cattacaccg cagatgttac gaaattgtta aaaatacttg gtattagtct
     3481 ttgaaaatgc tagcattgta tatataagtt aacagaataa taacatgtta tgaggggcag
     3541 aatatgaatg agagacgcag aatttagagc gatgcttcag gcatcaagag aacgcaacaa
     3601 gcacaactcc tacgcttaca caaacaaccc caccagctac gaagtcccaa cct
//
LOCUS       DADMGC010000233         3616 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.234, whole genome shotgun sequence.
ACCESSION   DADMGC010000233 DADMGC010000000
VERSION     DADMGC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.234"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(143..871)
                     /locus_tag="JNZ73_004105"
     CDS             complement(143..871)
                     /locus_tag="JNZ73_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAY8734157.1"
                     /translation="MSQQEISVINLDQLVSMTSVEIAELTGKEHRNVLRDIRNMAEEL
                     NALKTEQCSKLSSHIGVTEDVYLNAQGKQQPLYRLDRKHTFILVAGYSVHLRAKCYDH
                     IQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKN
                     PISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKMRGILIA
                     FTGTDQQKQEHLLKLAQSGKVEGI"
     gene            complement(1834..2487)
                     /locus_tag="JNZ73_004106"
     CDS             complement(1834..2487)
                     /locus_tag="JNZ73_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAY8734158.1"
                     /translation="MAEYYPAEFGGTAFNCPNCGAYSQQRWVRLKYGANGIYETPFAF
                     SKCERCNEKAYWYDETMIIPASANVEMPNPDMPDDCKSDYMEARSIVNLSPKGAAALL
                     RLSLQKLMVHLGEPGKHIDTDIKSLVAKGLSPLVQRAADICRIVGNQAVHPGEINIDD
                     DPQLAHGLFKLLNIIVTEQITRPKEVEAMFNSMPERALKGIEDRDRKAREQQQAASE"
     gene            complement(2480..>3616)
                     /locus_tag="JNZ73_004107"
     CDS             complement(2480..>3616)
                     /locus_tag="JNZ73_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY8734159.1"
                     /translation="YLEPETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQ
                     NWKPATLSENRASHSTLIEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSM
                     PLSDLLNRESKTDCLDVVTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKA
                     SDARDAFSPEQVGQLLVAAKAYSQKNAGKPYHYYVTALAAITGARLNEVAQLQVKDVR
                     TTEAGTVFIHINEDDSSLPGKSIKNAHSDRCVPLVDGAYGFVLADFISLVEDRRKIEG
                     DNAMVFDGLKLMKNGYGEQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVE
                     LAYAQAIMGHSSGSITYDRYAKEVEVDRLVNVMAGVYKETGVNG"
BASE COUNT          865 a          921 c          898 g          932 t
ORIGIN      
        1 ggctattagc tcgtctcggg attggtgctg gcgggtctca tcttccgggt ctagaccaag
       61 ctctctgagg aggacttcag tccggtagcc acggtaacgg agagtcatgc gcccctcagg
      121 tgtgcgcggg tcaatcatca tcttagattc cctccacctt accggattgt gccagcttca
      181 ggaggtgctc ttgcttctgc tggtcggtgc cagtgaatgc aataaggatg cctcgcatct
      241 tgtcaaccat ctcaggctta ccgaagacct ccagcccctt agcgttgaac cggaagttat
      301 acccacctgc tgagaacttc tggtctgacg gcttaatgat acgaaagcag tcggaatact
      361 tcggatttac agaccactcg ttactgattg ggttcttacg aaagaggcca cgatgctgga
      421 gaagtgcggt cattagcttc tgagcgggca gaccataggt tttgcagtag tcgccccaag
      481 tcacgcctcg gcggttagcg gactgaatgg ctgctcgctt cttctggtct tccagttgaa
      541 ggactcgacg ctctaacgtc tgaatatggt cgtaacactt ggcgcggagg tgaacggaat
      601 agccagcgac cagaatgaaa gtatgcttac ggtcgagacg atacagcggc tgctgtttcc
      661 cctgcgcgtt tagatagaca tcttcagtca ccccgatatg actgctcagt tttgagcact
      721 gctcagtttt gagcgcgtta agctcctcag ccatattgcg aatatcacgc aggacatttc
      781 ggtgctcctt gcctgtcagc tcagcaattt caactgaggt catggagaca agctggtcga
      841 ggttaatgac gctgatttct tgttgggaca tttacgtgta ctccttttgt ttgcaccctg
      901 aagtcgcacc ctactgacct actcaagccc ccacgtaaag ggagaccagc aggcaacagg
      961 acgcaaacag aatgacttga ggtaattgtg atgtggtctg cgatgaccag tagtgataaa
     1021 ggtgcaagac agcatgtccg acgtccagat ggtgagcggc gatagaccca agtcaaaatg
     1081 aagacacgag aacgctaacg ggctgggagt ctgacagagg ctgtcttgct agccttggta
     1141 tggagaaaga cttaatgtcg gggggcttcc gcataacttg acgcatcaag accagaggcg
     1201 gaatgtatgg cccctatagt gatagctaag ggtccattcc ctatagtgag ggtataagat
     1261 ttgctacctg ataaggccat aatctgacct ccagttagac ccgaagtatg gagaccacat
     1321 catagcgacc atagtttact tcgggatgac aggaggctgg acttaaagtt cttcttattg
     1381 gaattgtgat aaacacaatc cataacaaga taaactcatg gtctcctcca gcctgctggt
     1441 caggactcaa ggttcaacct cagacaccac tggtctaacc tccttgagtt tccgcctgtc
     1501 cacaatggat ggcagacctc agcaatgatg tcttggttct tcacaatctc gcacaccgat
     1561 ggtctcatag acggaagttc cgctgaggtc tgatgttctc cgcctgtcaa aggagagaga
     1621 ttgctacgtt aaagggaatg gtctcaggcc aaggtgattt aacactaggc gtctgcggat
     1681 ggtttccaat cggtccgcta ccggaagcct gagacttaat gaatgtgtgt gggtcgtgaa
     1741 tgttattaag gtggggactt agggtctctc ccctatagtg atagttaagc ccccttccct
     1801 atagtgaggg tatattcaat ctgacctaaa gctttactcg ctagctgcct gctgctgttc
     1861 cctcgccttc ctgtcccggt cctcaatgcc cttgagcgcc ctttccggca tgctgttaaa
     1921 catggcttcg acttctttcg gtctggtaat ctgctcagtg acaataatgt tcagcagctt
     1981 aaaaagacca tgcgctaact gagggtcgtc atcaatatta atttcacccg gatggaccgc
     2041 ctgattgcca acgatacgac aaatatcagc agctcgctgg accaatggag acaatccttt
     2101 ggctacaagc gacttaatgt ccgtgtcgat atgcttcccc ggctcaccga gatggaccat
     2161 cagcttctga agacttaagc gcaggagtgc ggcagcgcct ttaggagaaa ggttaacaat
     2221 gcttctggcc tccatgtagt cggacttaca gtcgtctggc atgtctgggt taggcatctc
     2281 aacgtttgct gacgctggta taatcatggt ttcgtcatac caataagctt tctcattgca
     2341 tcgttcacat ttagaaaagg cgaaaggtgt ctcataaatc ccgttagcgc catattttaa
     2401 gcggacccag cgctgttgag aataagcgcc acaattcggg cagttaaaag cagtgccacc
     2461 aaactctgcc ggataatact cagccattta ctccagtctc cttataaaca cccgccatca
     2521 cattaaccaa tctatctacc tcgacctctt tcgcgtaacg gtcgtaagtt atagacccag
     2581 agctatgacc cataatcgcc tgagcatacg ccaattcgac cccatgttgt tttagctggg
     2641 tcgcaactgt atgtctaaac gagtgaaaag ctaagccgct acggtcagca aggactttag
     2701 gtagcaacgt tcggttaaac catttgctca cctgctcacc gtagccgttc ttcatcagct
     2761 tgaggccatc gaataccata gcattatcac cctcaatctt tctacggtct tctacgagtg
     2821 atataaagtc agccaataca aaaccataag caccatcgac cagcgggaca cagcggtcac
     2881 tatgggcgtt cttgatactc ttacccggca ggctgctatc gtcttcgttg atatggataa
     2941 acacggtccc ggcctcagtg gtcctgacat ctttgacctg aagttgggcc acctcgttaa
     3001 gtcttgctcc agtgattgca gccagagcgg tgacgtaata atggtatggc ttacctgcgt
     3061 tcttctgaga gtacgcttta gcagcgacca gtagttgccc tacctgctca ggactgaacg
     3121 catcacgagc gtcggaggct ttacgctgag ggactttcag ctcaagacct tcggtcaggt
     3181 tcttagctat caagtcgttg cgtaccgccc acttaaacac agcagccatc ttgataagat
     3241 atttattgtt aatggtaacg acgtccagac agtcagtctt agactcccgg ttcagcaagt
     3301 cagacagcgg catagactta aagcgttgct tgcggttctt cggtagctgc tggaggactt
     3361 cacgaactct gagcatatct gcacgggtcg ccttgccgac atcctgaata tccagatagt
     3421 cgaatatctc gattagcgta gagtgtgacg ctcggttttc gcttagggtc gctggcttcc
     3481 agttctgggc gttctcctgc tcgtactgct caatgagaga cgcgagggtc agtgctttag
     3541 gttcagggac ttcaggttcc gccaatacag atagggagac ggacggtctt agactgccag
     3601 tctcaggctc aaggta
//
LOCUS       DADMGC010000234         3586 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.235, whole genome shotgun sequence.
ACCESSION   DADMGC010000234 DADMGC010000000
VERSION     DADMGC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.235"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            28..489
                     /locus_tag="JNZ73_004108"
     CDS             28..489
                     /locus_tag="JNZ73_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HAY8734160.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(530..1000)
                     /locus_tag="JNZ73_004109"
     CDS             complement(530..1000)
                     /locus_tag="JNZ73_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAY8734161.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(1047..1766)
                     /gene="yehT"
                     /locus_tag="JNZ73_004110"
     CDS             complement(1047..1766)
                     /gene="yehT"
                     /locus_tag="JNZ73_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HAY8734162.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1763..3448)
                     /gene="btsS"
                     /locus_tag="JNZ73_004111"
     CDS             complement(1763..3448)
                     /gene="btsS"
                     /locus_tag="JNZ73_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HAY8734163.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          952 a          925 c          818 g          891 t
ORIGIN      
        1 tcttgattaa ataaaatgga gttttacatg aaggctttca ataagctgtt ttccctcgtt
       61 gttgcatctg ttctggtttt ctctcttgct ggctgcggtg acaaagaaga atcgaagaaa
      121 ttcagcgcca atctgaacgg cactgaaatt gccattacct atgtctacaa aggtgacaag
      181 gtgcttaagc aatcttctga aaccaaaatt caatttgcct ccattggtgc aaccaccaaa
      241 gaagatgctg ccaagacact tgagccgtta agcgccaaat acaaaaacat cgcgggtgtt
      301 gaagaaaaat caacctatac cgatacctac gcgcaggaaa acgtgactat cgatatggaa
      361 aaagtggatt ttaaagccct gcagggtatt tcaggaatca acgtttctgc tgaagatgcc
      421 aaaaaaggta tcactatggc gcaaatggaa ctggtgatga aagccgctgg ttttaaagaa
      481 gtgaaataat ctgtcggcgg ccatgtttcg catggccgcc atacccgtct tagcttttct
      541 tcacatgctg gcgcgctgcc agtccacgca gaaaataacg taaaaattga tcgccgcatt
      601 cgcggaagtt tttatgatcc ggtgcgcgca tcatcgctgt aatttccggc atcgaaacgc
      661 ggaactgctg ttcggtgagg atagccagaa tgtcatcggt tttcagcgaa aacgcgatgc
      721 gtaatttttt cagcacgatg ttgttattaa tgcgacgttc cggctccagt gccggagcag
      781 actcatcctt gccgcgtttt tcataaatca ggccattgag gaatgacgac aaaacaatgt
      841 ccggacaacg ctgaaaaccc tcttcgtctt ctttacgtag ccagacggcg atctgttccg
      901 cggtggcttc gacattatcc agcgccagaa tacgcaccag gtcattatta ttggctttca
      961 aaatgtagcg cacgctgcgc agaatatcgt tacttatcat gaggccttca ggtgttgatg
     1021 aggcaaaaag ccattttagc agtcttttac aggccaatcg cctcttttaa gcttttcaga
     1081 taacggcggc tgaccggcac ggttaagcca ttacgcaaaa tcaactcggc ctggccgtta
     1141 tcttccagac gaatctcctg taaatgcgcg aggttaacca gatactgacg atggcagcgc
     1201 agtagtggtg tacgactttc cagggtacgt aatgtcaatt cggtaaagcc ctctttccct
     1261 tcgtggctgg taacgtagac accgctcatc cgactgctga caaatgccac atctttcatt
     1321 tgcagcaaat aaatccgact atgccccgta caagggataa atttcagcgc ctgttgattt
     1381 tccggtaaca gcgaaacatc ctgcttgctg cgctcctgac gcaatcgcgc cagcgttttc
     1441 tccagtcgcg cttcatcaat tggcttcagc agataatcaa aggcatgttc ttcaaaggct
     1501 ttaatagcgt attcgtcaaa cgcagtgaga aaaacaatat acgggcgatg ttccgggtca
     1561 agcatcccca ccatttccag accactgatg cgcggcatct ggatatcgag aaacagcaca
     1621 tccgggcgca gtttatgcac cgcgccgatc ccttccacgg cgtttgaaca ctctccaacg
     1681 atttcaatat cgctctgctc ctgcaaaaat atacgcaggt tctcccgtgc taacggttca
     1741 tcatcgacaa ttaagacttt aatcatgcct cgtccctcca tggtagtcgt aacgttattc
     1801 gggtgtaact atcaggctca caggcgacgc ttattccata gtcatcgcca aaccgttcac
     1861 gtaaacgctt atccaccaga ttcatcccca gcccactggc attggttacc ggttgataca
     1921 aaccggcatt gtcttcgatc tccagcatca aatgttgccc ctcacgtcgg gcgctgattg
     1981 ccactcgccc tgtatccagc agttgtgatg tcccatgttt aatggcgttt tccactatcg
     2041 gttgcagggt aaacgcgggc aattgctgct gggataattc ttgcggaata gcaatgttga
     2101 cctgcaaccg cgactggaag cgcgcctttt caatttgcag ataagcgttc acatgttcaa
     2161 tttcgtcggc gagagtaaca aactccgaag gccgctttaa gtttttgcgg aaaaaagtgg
     2221 aaagatactg caccagctgg ctggcctgtt cgctgtcgcg gcggatcacc gctttaatgg
     2281 tgttaagcgc attaaacaaa aaatgaggat tcacctgggc gtgaagcagt ttgatttctg
     2341 actgggtgag catcgctttt tgccgctcat attgcccggc aagaatctgc gccgaaagca
     2401 gttgcgcaat cccctcgccc agcgtgcggt ttattgaact gaataaacgg tttttggctt
     2461 catacaattt gatggtgccc atcacgcgct gattttcgcc acgcaacgga attaccagag
     2521 tcgaccccag tttgcattgc ggatgcaaag agcaacgata aggtacttcg ttgccatcag
     2581 cgtagaccac ttcaccggtt tcaatcgctt ttaaggtgta agtcgaagaa atcggtttgc
     2641 cgggtaaatg gtggtcgtca ccaattccgg taaaggccag caatttctct cgatcggtaa
     2701 tcgcgactgc accaatatcc agctcctgat acagcacctg agccactttc atgctgttca
     2761 cttcgttaaa cccctgtcgc aaaatgcctt ccgtcgaggc tgccactttc agcgcagtgg
     2821 cagaaaaagc cgaagtgtat ttttcaaaca tcgcgcgttt atcgagcaat atacgcataa
     2881 acagcgccgc gccgacggta ttggtgacca tcattggcgc agcaatatta ctcaccagac
     2941 gcaccgcatc ttcataaggt cgggcgatcg caaggatgat cagcatttgc accatttcag
     3001 cgacgaacgt gacggcaccg gcggtaatgg ggttaaagac tttatcagtg cgcccgcgac
     3061 ggatcaggat gctgtgtacc aggccgccga gcaatccttc aacgatggtt gagatcatgc
     3121 aacttagcgc ggtcatgccc cccatcgaat atcgatgtaa gccgccggtc agaccaacca
     3181 gcccaccgac gaccggaccg ccgagtaagc cgcccattac cgcgcctatc gcacgggtat
     3241 tggcaataga atcgtcaatg tgcaacccaa accaggtgcc catgatgcag aagatggaaa
     3301 agacgatgta gcagagaaat ttatgcggca gacgaaccgt gacctgcatt aacggtatga
     3361 ataatggcgt tttactcatt aaccacgcaa tgactaaaaa aacgcacatc tgctgaagca
     3421 gcagcaacac cagattaaaa tcgtacatac ccgcaaacca cacttccctt taaaacgcgt
     3481 aacatacatt gcctgcgttt aactttcttt gaactcttgc agaaaaatga gaattcgtga
     3541 gtacgatcac tcaaaatcgc ctggcaaaaa taaaatcacc ctatag
//
LOCUS       DADMGC010000235         3517 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.236, whole genome shotgun sequence.
ACCESSION   DADMGC010000235 DADMGC010000000
VERSION     DADMGC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.236"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(63..1505)
                     /gene="cusS"
                     /locus_tag="JNZ73_004112"
     CDS             complement(63..1505)
                     /gene="cusS"
                     /locus_tag="JNZ73_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HAY8734164.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPD"
     gene            complement(1495..2178)
                     /gene="cusR"
                     /locus_tag="JNZ73_004113"
     CDS             complement(1495..2178)
                     /gene="cusR"
                     /locus_tag="JNZ73_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HAY8734165.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2335..>3517
                     /gene="cusC"
                     /locus_tag="JNZ73_004114"
     CDS             2335..>3517
                     /gene="cusC"
                     /locus_tag="JNZ73_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HAY8734166.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKMEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQ"
BASE COUNT          862 a          911 c          921 g          823 t
ORIGIN      
        1 ggtatgacta ttgcggcacg ttatttttac actggttata aaagttgccg tttgctgaat
       61 aattaatcgg gtaatgtgat aacaaacctt gtcccccgcg catctgacgt taccgcaacc
      121 gtgcctttat gcgcgacaac aatcgatttc actatcgcca gcccaatacc gctaccttca
      181 ccttttcgct ggcgggaggg gtcaacgcga tagaaacggt caaacaatcg cggtaagtgc
      241 tcgggcgcaa tgggcgtacc ggggttttcg acggaaactt gcacctggtg atcgaccgtc
      301 tgacagcgca ctacaattgt ctctctggtt ggcgtataac gcagggcatt agaaagcagg
      361 ttgcttagcg cccgacgcag catcaacgga tcgcccgcaa cctgacgttt gtcgccaaca
      421 aaccgcaact ccacgccgcg atcttccgct agcgcctcga aaaaatcgaa cactttgccg
      481 acttcatccg ccaggttgag cattttcttt tcggggatta gctggttgtt atcggcctga
      541 gcgagaaaca gcatatcgct gaccattttc gccattcgcg tcagctcttc gagattagag
      601 tagagcacat cttccagctc cttctggctg cgcgactggc tgagggcgat ttccgtttgc
      661 gtaatgagat tagtaattgg tgtgcgaatt tcgtgggcga tatccgctga gaaattggac
      721 tggcgagtaa agacatcctc aatacgctcg atcatatggt tgaacgacag taccagctgt
      781 tccagctcaa tcggcacggt ctgcgggtca aggcgaacgt cgagatcttt cgaggtgata
      841 ttctggattt gacggctgac gctgcggatc ggcgcgtgac ctttatgtac cgccaacagc
      901 acgataaaga cgatcaggat gctgattacc gatgcggtca taataagttt attcatcaaa
      961 tcatttatgt aatgaagatg aaaatcgatc gacagcgcga tgtagagcgt ataaatcggt
     1021 ttgccgtcca ccaacgggcc aaccggcaag ttaatcatcc gccagttgct gtgttccata
     1081 tgcccgtgac cgtggcctgg catcataatc gtcgggccgg aaaggagata cacctcgcca
     1141 ccccgcgcgt ctttatcggg tatggcatca cgtgcaaact cgcggatatc aggcgcaccg
     1201 ggggagtgat acaccgtttt gccgtggcta tctgccaggg aaatcaacac gttggaataa
     1261 ccactgacga tatcttccag cgtcattaag cgtcgggctt gcgtttcgtc agggtgattt
     1321 agtacccgtt caagggtggc gctaatctct tttaaatcat taatgtcctg ctcggcaaaa
     1381 tgcactttta ctgagtggat catgatccat gcaaaggcga aaaacgccgc gatggtggcc
     1441 aggctgataa aaaaggtcag gcgggttgcc agcgaaaacg ggcgctgaaa tggcttactg
     1501 accatccggc acctcaagca tgtaacccac gccgcgcacg gtttgaatca gcttcggctc
     1561 aaagtcgttg tcgattttgc cgcgtagccg cttcaccgcc acatcaatgg cgttagtgtc
     1621 gctgtcgaaa ttcatgtccc atacctgcga ggcgataagc gagcggggca gcacttcgcc
     1681 ctgatggcgg agaaaaaact ccagcagagt aaattcttta ctggtcagag tgatgcgcgt
     1741 gccgctgcgg gtgactttgc ggctgacgag atcgaccatc agatcggcaa cctgaaactg
     1801 actttcgata atcaccgccg ccccgcgccg caggagagtg cgcacccgcg ccagcagttc
     1861 agcaaaagcg aacggtttca ccaggtagtc atctgccccc aactccagcc ccttgacgcg
     1921 atgttcaatg gtgccaagcg cggtaagcaa cagaatcggc atccctttat tggcggagcg
     1981 caacatgcgc acgatatccc agccgttcac gtctggcagc ataatatcga ggattatcag
     2041 gtcataatca ccggtcatcg ccagatggta gccattcagc ccgttgtcgg ccaaatcgac
     2101 cacaaaaccg gcttcggtta accctttggt caagtattct ccggttttct tttcatcttc
     2161 gacaatcaac agtttcatat ttcctccgca tgttgcccgg gcaattctag agtagcggga
     2221 tcagatggca atcgcttatt ggcaaaatga caattttgtc attttcctgt caccggaaaa
     2281 tcagagcctg gcgagtaaag ttggcgacat aaaatcacca gaaattatga gcctatgtct
     2341 ccttgtaaac ttctgccatt ttgtgtggcc cttgcgctaa ctggttgttc actggcaccg
     2401 gattatcagc gtccggtaat gcccgtaccg cagcagttct cactcagcca gaacggcctg
     2461 gttaacgcag cggataacta ccagaacgcg ggctggcgca ccttttttgt tgataatcag
     2521 gtgaagacgc tgattagcga ggcgctggtg aataaccggg atttgcgcat ggcgacgctg
     2581 aaagtgcagg aggcacgggc gcaatatcgt ctgaccgatg ccgaccgcta cccacagctc
     2641 aatggcgagg gcagcggcag ctggagcggc aatcttaaag gcgattcagc cacgacgcgg
     2701 gagttctcga ctggccttaa cgccagcttt gatctcgatt ttttcggtcg cttaaagaac
     2761 atgagcgaag ccgagcgcca aaattattta gccactgagg aagcccagcg cgcggtgcat
     2821 attttgctgg tttctaatgt cgcgcaaagc tatttcaatc agcagctggc gtatgcgcaa
     2881 ttgcaaatag ccgaagaaac gctgcgtaat tatcagcagt catatgcgtt tgtcgaaaaa
     2941 caactgttga ccggtagcag caatgttctg gcgctggaac aggctcgcgg ggtgatagaa
     3001 agtacccgca gtgacatcgc taaacgtcag ggggaactgg cgcaggcgaa taatgcattg
     3061 caactgttat tgggaagcta cggcaagcta ccgcaagcgc agacagtaaa cagcggcagc
     3121 ctgcaaagcg ttaaattacc ggcgggcttg tcgtcgcaaa tcttattgca gcgcccggat
     3181 attatggaag ctgaacacgc gttaatggcg gctaatgcta atattggtgc tgcgcgtgcg
     3241 gcatttttcc cgtctatcag cctgaccagc ggaatatcaa ccgccagcag cgatctgtcg
     3301 tcattattta acgctagcag cgggatgtgg aattttattc ctaaaatgga gatccccatt
     3361 tttaatgccg gacgcaacca ggccaatctg gatatcgccg aaattcgcca gcagcagtcg
     3421 gtggtgaatt atgaacagaa aatccagaac gcttttaaag aggtggcaga tgcgctggca
     3481 ttacgtcaaa gcctggacga tcaaatcagc gcccagc
//
LOCUS       DADMGC010000236         3380 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.237, whole genome shotgun sequence.
ACCESSION   DADMGC010000236 DADMGC010000000
VERSION     DADMGC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.237"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..934)
                     /locus_tag="JNZ73_004115"
     CDS             complement(<1..934)
                     /locus_tag="JNZ73_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HAY8734167.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLAKNSRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFIVYSFACFFIGKFIANILM"
     gene            complement(962..1882)
                     /gene="rbsK"
                     /locus_tag="JNZ73_004116"
     CDS             complement(962..1882)
                     /gene="rbsK"
                     /locus_tag="JNZ73_004116"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY8734168.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKGDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGMQSSYPSIEQFNEFLTLNE"
     gene            2188..2970
                     /locus_tag="JNZ73_004117"
     CDS             2188..2970
                     /locus_tag="JNZ73_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAY8734169.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPALSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(2972..3070)
                     /locus_tag="JNZ73_004118"
     CDS             complement(2972..3070)
                     /locus_tag="JNZ73_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HAY8734170.1"
                     /translation="MISQIDKLEYVVKVQRNQSNPTMFNKIVVFFQ"
BASE COUNT         1002 a          772 c          671 g          935 t
ORIGIN      
        1 tcataaggat gttggctata aacttgccaa taaagaagca ggcaaagctg tagactatga
       61 agtttgaagc atcacgttcg ttgatatcgc ccaactccag tgccagacgg atggtaaatg
      121 accatactgc gacctgcata cccacataaa ggaattgagc cacaatacca cgacgaaagc
      181 gcgaattttt agccagatag cgcagcgtat ccattgctga cgggcgttta tggtgacttg
      241 tctgtgccac tttacaggtt gggaagcggg ttaaaaggaa caacaccatg accacaacca
      301 gaatcataat catatactta tacggttcaa gggtgttctc taacatcagc accttaaagt
      361 tgtgaatttg ctcggcgttc attccggaca tctgcttctc aaggctttcc ccctcggaga
      421 aaaccagata tttgcccaat aaaataccag acgcagcacc aatcggataa aaggtctggc
      481 tgatattgag ccgcaatgtg gcataggctt ttggaccgat cattgaactg tatgtgttcg
      541 ctgcagtttc aaggaaactc aggccaatcg caatcgcaaa aatagctgca aggaacatag
      601 tgtaggttgc catatgcgag gcagggaaaa aaagtgtaca accaccaata tacagcgtca
      661 ggccaattaa aattgccacc ttataactgg tctttttaat cacaagggat gctggtattg
      721 caattaaaaa ataacctcca taaaatgcgc tctgcaccaa tgctgaagca aagttactta
      781 gcgaaaatac acttttgaat tgagtgatta atatgtcatt taatgcagct gcgcatcccc
      841 atagcgggaa taaacacgat aacaaaataa actggaacaa gggagtctta ttcagatacc
      901 catcaggcat ctgaatgatg tttttatcgt tcatagtgct acctttaact gtgcaggatg
      961 attattcgtt taaggttaaa aattcattaa attgttcaat actcggataa gatgattgca
     1021 tacctttccc tgtgacgctg aaagcggcaa agagagcggc ttttttcaaa gcggcttcaa
     1081 catcaccgct ttgaacataa taatgggaaa agcaaccaat aaatgcgtca ccagcgccac
     1141 tagtatcaac agcatttact ttgaatgcag gaacatggac ttcctgatcg cgggtcatcc
     1201 ataatgcgcc tttttcgctc atggtaacaa taatattgtt cagcccttta tcaactaacg
     1261 aacgtgcggc caaacgaata tgatcataag tatcaaccga cataccagtt aatatttcca
     1321 gttctgtttc attcgggata aagaaatcac ctttgcaggc ataagacata tctaactcac
     1381 gcaatgccgg agccggattt aataacactt caataccatt tttcttacca aactcaatcg
     1441 cgtggtaaac tgtttccagt tgaacttcca gttgtaaaac gatcaatttg cattttttca
     1501 gatcttctgc agctcgatcg atatcttccg gggaaagaaa tttattcgct cccttaatta
     1561 ttaatatact attgctcgag ttggcattaa caaagatcgg tgcaacacca ctgctggtac
     1621 aggggacctt ctcaacataa gtggtattaa ttccccatga ttcaagatta cgaatagtat
     1681 tatccgcaaa aatatcatca cctactttag tcagcatcag gacttttgaa ttcaatttag
     1741 ccgccgccac cgcttgatta gcacctttcc caccacatcc gattttgaag gcaggtgctt
     1801 ccagagtttc tccttcttta ggcatctgat tagtgtaagt aatgagatcc accatattgg
     1861 aaccaataac tgcaatgtcc atttcactac ctcttataaa ctttcgcata acaatggtat
     1921 ttaaataaca ttagcatgtt acttttgcat catttgtgac tgagatcgcg attagcacat
     1981 caacccgatg tttatttaat agacttccag tctcatcact caggccaaca ctatctaatc
     2041 ataagcaacc taacaagatt agtgcccaaa attcagcagc ctataccctt ttcatttcaa
     2101 aggggcggtc gtatagtatg gtaatgaaaa caatgtttac taacgccaaa atgttatttt
     2161 tataacattc ttacggagag agagttgatg gaaacgaagc aaaaagagcg tatccgacgt
     2221 ttgatggaac tgcttaagaa aaccgacaga atccatttga aagacgcagc gcgaatgctg
     2281 gaagtttctg taatgactat tcgtcgcgat ctccatcagg aagatgaacc tctgccactg
     2341 accctactgg gtggctatat tgtaatggtg aataaacccg cgctatccat gccagtaatc
     2401 catgacgttc caaaaaatca tcgtgatgac ttacctattg caattctggc tgccggaatg
     2461 gttaatgaaa atgatctgat cttctttgat aatggccagg agataccact cgttataagc
     2521 atgatcccgg atgcaatcac cttcaccggc atctgttact cacatcgcgt ctttgttgcg
     2581 ttgaatgaaa agcctaatgt aacagcaata ctttgtggtg gtacgtatcg tgccagaagt
     2641 gatgcttttt acgatgccag taactcttcg ccattagact ctctcaatcc gcgaaaaata
     2701 tttatttccg ccagcggtgt gcataatcac tttggcgtca gctggtttaa ccctgaagat
     2761 cttgccacta agcgtaaagc gatgaaccgt ggactacgga aaattttgct cgcccgccac
     2821 gcgttgttcg atgaagtggc ctctgccagc ctcgcaccga tctctgcatt tgacgttctg
     2881 attagcgatc gtccgttacc ggcagattat gttacgcact gccagaatgg ttctgtaaag
     2941 atcattacac ctgattcaga agacgaatga cttactgaaa aaacaccaca atcttgttaa
     3001 acatcgtcgg gttggactga ttacgttgca ctttcaccac atattccagc ttatctattt
     3061 ggcttatcac ctactccaga cgctggtcat ccttgaccag tagccagata tggcttttgt
     3121 cgcagtcctg aatcggcagg cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag
     3181 cccacaaagg tgagtcatta cacccggatg gtaaacaggc acttccttgt tgtagaagcc
     3241 gttatattgt ccataatgtc tgggttttta atgcatactg ggtaacaacc ggacgaatct
     3301 tcgaatatca ctggcgctgc cagccggttt gaattcatct cacaaccctg catagagcga
     3361 atctcttgcc tgcacgtcac
//
LOCUS       DADMGC010000237         3164 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.240, whole genome shotgun sequence.
ACCESSION   DADMGC010000237 DADMGC010000000
VERSION     DADMGC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.240"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..432
                     /locus_tag="JNZ73_004119"
     CDS             <1..432
                     /locus_tag="JNZ73_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734171.1"
                     /translation="RARDDQNAGCQTDYVHAAIIADQMMSNASELRGLHGDLHHENIM
                     FSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIAQMADAFSRALDVDPR
                     RLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
     gene            complement(404..943)
                     /locus_tag="JNZ73_004120"
     CDS             complement(404..943)
                     /locus_tag="JNZ73_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY8734172.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFGVVCGKGRNPTLRLWPAIFSGEIDV
                     FPGDRRSRLISSMTSVAPA"
     gene            complement(987..1376)
                     /locus_tag="JNZ73_004121"
     CDS             complement(987..1376)
                     /locus_tag="JNZ73_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734173.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            1546..2034
                     /locus_tag="JNZ73_004122"
     CDS             1546..2034
                     /locus_tag="JNZ73_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HAY8734174.1"
                     /translation="MFCCYNTVHTGDTVTFMAWHTVQETLKLTGKSRSQLYRDMAKGL
                     VSYRTGKNDRREFETSELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKL
                     DALQQTVTLMLEDKMAREAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSG
                     SE"
BASE COUNT          864 a          799 c          789 g          712 t
ORIGIN      
        1 cgggcgcgcg atgatcaaaa cgcaggttgt caaactgact acgtccacgc ggcgattata
       61 gccgatcaaa tgatgagcaa tgcctcggaa ctgcgtgggc tacatggcga tctgcatcat
      121 gaaaacatca tgttctccag tcgcggctgg ctggtgatag atcccgtcgg tctggtcggt
      181 gaagtgggct ttggcgccgc caatatgttc tacgatccgg ctgacagaga cgacctttgt
      241 ctcgatccta gacgcattgc acagatggcg gacgcattct ctcgtgcgct ggacgtcgat
      301 ccgcgtcgcc tgctcgacca ggcgtacgct tatgggtgcc tttccgcagc ttggaacgcg
      361 gatggagaag aggagcaacg cgatctagct atcgcggccg cgatcaagca ggtgcgacag
      421 acgtcatact agatatcaag cgacttctcc tatcccctgg gaacacatca atctcaccgg
      481 agaatatcgc tggccaaagc cttagcgtag gattccgccc cttcccgcaa acgaccccaa
      541 acaggaaacg cagctgaaac gggaagctca acacccactg acgcatgggt tgttcaggca
      601 gtacttcatc aaccagcaag gcggcacttt cggccatccg ccgcgcccca cagctcgggc
      661 agaaaccgcg acgcttacag ctgaaagcga ccaggtgctc ggcgtggcaa gactcgcagc
      721 gaacccgtag aaagccatgc tccagccgcc cgcattggag aaattcttca aattcccgtt
      781 gcacatagcc cggcaattcc tttccctgct ctgccataag cgcagcgaat gccgggtaat
      841 actcgtcaac gatctgatag agaagggttt gctcgggtcg gtggctctgg taacgaccag
      901 tatcccgatc ccggctggcc gtcctggccg ccacatgagg catgttccgc gtccttgcaa
      961 tactgtgttt acatacagtc tatcgcttag cggaaagttc ttttaccctc agccgaaatg
     1021 cctgccgttg ctagacattg ccagccagtg cccgtcactc ccactcttac attgttcgca
     1081 gtatctaggt acatgttatt gcgtgcacct tcatgatgta taatgttatt aatgacagca
     1141 gaaagatcga cagaattaat attcttcgta cctgggacag ttatcgggtg tcggccagtg
     1201 atgagaccaa aggatgcagt ataagatgaa ccactactat tatcatggcc accattacca
     1261 ccaccgttaa aactacctcc cgaacttcca gagccgctat gaccatcatt gtaggggtca
     1321 tttggtccca tgtgaccaaa accaccgaca gtaccgctat ttaactctgt actcataaaa
     1381 ttatcctctt tgacttttaa aaacagcaaa aactgtattt ataacaatgt gtttatatac
     1441 agtattcgaa atactacgcc cgtcaaaacc acaccgtcaa caggtttttt gtgtattttt
     1501 cacgcctcac gggcagcctc agaccttttg tgttaaaaca gtaatatgtt ctgctgttac
     1561 aatacggtac acacaggaga cactgtcact tttatggcat ggcacacagt tcaagagaca
     1621 ctgaaattaa caggaaagtc gcgctcgcaa ttatatcgtg acatggctaa aggccttgtc
     1681 agctatcgca caggaaaaaa tgatcgccgg gagttcgaga ccagtgaact gattcgggcc
     1741 tatggcgagc tgcgcacttc tgagacacca aaaaggcaca aggagggaca ctccaaatct
     1801 gtcgttagcc aggcggaaaa tgctcagcta gacgtcattc agcagaaact ggacgctctg
     1861 cagcagactg ttaccctaat gcttgaagac aagatggcgc gggaagcaga aaaccgcctg
     1921 cgaaatgagg agagggaaca actacaggcc gaaattagcc gtctggctga cgcgctggca
     1981 caggaaaaaa agcggggatt ctggtcgcgg ctgtttcgct ccggttcaga gtaacagtgc
     2041 aggtgactct gatttaccat aacatctgtt atcagcaccg cctgagaggc cctcagggcg
     2101 tttctgacgg gaaaccggta aaacaggcaa caaaccaccc gaaaacccgc cagaaacgcg
     2161 caggctctgt ttttacggca tgcatgagta tgcgcacaag tgcatagcgc ggaaattcac
     2221 accattcagc cggggtaagt cctgtttccg gcgttcttcc catccgaaaa cggtgaacgg
     2281 ggacgcaatt tattgcgtgg ccgtgaagcg ttgccggcgc agccggaaaa cccgctgtca
     2341 gcacgttcag gaagggggtg atgtacaaat ggcacagagg atgaaagtga cgtatgagct
     2401 gaaggaatga tgtaaaagtg atgtataagt gatgtaaaag tgatgtataa gtgatgtaaa
     2461 agtgatgtat gaactgaagg agcgatgtaa ttctgtagtc aggaagaata acccgttcat
     2521 gcaggcatga acgatacagt gaggaacgct ttttttgtca cgatctcccc ttaacctgcg
     2581 taaatcaccc gccatggttg attatttttc aacctcaaaa tggtcataca cctaacgcag
     2641 ccgcgatttt catcgcaact gcatgaatta aaagaaaaaa aaggaatatt tcgttttcac
     2701 gagtaatacc cgggtattaa aaatccactg taattacatg aaaataaaga aataaaaggt
     2761 aataccggag tattaccacg tacgcgggca agcgaaaaag ctataacgct tcccggccga
     2821 caggcgaccc aaaatccaca ggggcatgca ggttctcttc agtaacctgc gccattaaat
     2881 caaaaaggtc atattcgccg ggaagctgcg tatcagtcga atccagttgt tcatcatatt
     2941 cagatggcat ggggtgatat ccagtagctg agaggttgtt agcgggtttc ggggcgcagc
     3001 cctgaaccag tcatgtagcg ctagcggagt gtatactggc ttactatgtt gccacgattg
     3061 cgaaaatcag tgaagtgctt catgtggcag gagaaaaaag gcagcaccgg cgcgtcagca
     3121 gaatatgcga tacaggagat attccgcttc ctcgctcact gact
//
LOCUS       DADMGC010000238         3142 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.241, whole genome shotgun sequence.
ACCESSION   DADMGC010000238 DADMGC010000000
VERSION     DADMGC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.241"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="JNZ73_004123"
     CDS             complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="JNZ73_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HAY8734175.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGPRIYFSMSDGQ"
     gene            complement(1002..1097)
                     /locus_tag="JNZ73_004124"
                     /pseudo
     CDS             complement(1002..1097)
                     /locus_tag="JNZ73_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="JNZ73_004125"
     CDS             complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="JNZ73_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HAY8734176.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="JNZ73_004126"
     CDS             complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="JNZ73_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HAY8734177.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2194..3142)
                     /locus_tag="JNZ73_004127"
                     /pseudo
     CDS             complement(2194..3142)
                     /locus_tag="JNZ73_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          781 a          762 c          762 g          837 t
ORIGIN      
        1 tgttgtccgt cactcatgga gaagtaaatc cgtgggccgt ggtagtccgg cgaagaggtt
       61 aatctttgca gggcttgcag agcatgggat gataatgggt tatcactaat atgtattgaa
      121 cattcattcc tcagattaag aatgctttcc gggatatgac ttatctgatt tctatcaaaa
      181 aaatactctc tgacaacggg attatttccc tcagaaaatg ctggtagctc agtgaggaaa
      241 ttacgggtcg ctctgagatt tttaagagcc tgtgggagcg atggtaagga gactatttcg
      301 ttatagctga tattcatagt caataacgaa tcgggtaaag ctggcaggtt ttccagctca
      361 ttgtgttgta cctgaagtga ctccagcgca ggaggtagca cgggcaattc tgataattta
      421 ttgctggcgg aatataattt ttttagcgta acaggcagtt caggtaggtt agttaattga
      481 ttatatgata cattgagcag cgttatctga gctggtaagt tgtcaggtag cgaggacaga
      541 tttaaacggt ccagtcgaag ttcatcggaa ttattgataa gacattcttt aagtcgggag
      601 acagcctcat cacgctcttc accggggagc gcttgttttt cccatttatc ccatgctgaa
      661 aagtaatcag catatgtacc ggaaatagtg ttataaaaag aattttgggg caatgaaaag
      721 ttattattta tcggtaacat gatgggcatc cagttaaagc cggtggacaa atggctttgt
      781 taaaagaaat aagtaggagt ttttatgaag ttcattccat gaaatgaact tcagcacttt
      841 tttattataa taaggataaa caaaattggc tgtaaaaaaa cgatactgtt tcagttaacc
      901 gtgaatggcc tgtttgcacc gtattgttct tgtttttcgg ttagttaaac atcgtacttc
      961 atatttgaac ggtaagcggc tcgtcagaac cgtattgata tttactgaga ggtcagatca
     1021 actttccagg gcaacagatc gcgtacccgg tttgccggcc agtcctggat atgctcaatg
     1081 acgtaacgca gccattttgt caacgccacg atttttgacc ctagcctgac atttatattc
     1141 cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca
     1201 cttcttcccc gataacgaag atcggcacac tggccatatc agtggtcatc atgcgccagc
     1261 tttcatcccc gacatgcacc accgggtaaa gctcacggga gactttatct gacagcaggc
     1321 gggcactggc cagggggatc accatccgtc gtccgggcgt gtcaataatg tcactctgta
     1381 catccacaaa cagacgataa cgactctctc ttttataggc gtaaacctta aactgcattt
     1441 caccagtccc tattctcatc ggcaaaagag ccgttcattt caataaaacg ggcgatctca
     1501 gccatcccct gctgattttc agcctgccag cgttccgcac gcagacggcg ggcctctttt
     1561 tgcatggccg tatttaccag gcctgagata ttcacgtttg cggatttaag taactgatag
     1621 ctgtcactgt ctatagtgac agtaatacgc tgcttcatga taagccctca aaaacatact
     1681 tatgatatgt atatgagtat atattaatgg tacaaaccat caatatccat aaaaagtaca
     1741 ccattaattc gctataagtt tttaatacaa ttttttctgc tgcgattttt ttatactata
     1801 attagcatac ttataacacc gtaagtaaat tacatgaaag caggcacagc gtgatccgat
     1861 ttttctgcgt ataacgattt tccggaagtg taatacggtt gcgccccggg ccgggcaccc
     1921 ttttctgttg ttgtgtacgg gcccacagag aatggtaccg gaggcgggct cagaagtact
     1981 gagtcgggtg atcgcgtcag gccgcaaagt actgacacgg gtattggtga ctggtgatga
     2041 agtactgccc cgggtatgat ttcaggctga ttcaggttct gattctctgt cagtccgcgt
     2101 ggttgctggc ctggctgatt tcctgttgtc aggcgttcaa caaccgactg caggttatag
     2161 cccggttcac gaacacctga cggcaggtat cactcacccg gtgaaggtaa atccctcaag
     2221 aggcagcaac tctgccagtc gctcctccgg ccattccggc agacgcgtca ggacgtccgt
     2281 cagccaggca tgtggctcca gaccattacg ttttgctgtt tccagaaggc tcattatctg
     2341 tgccgcccgg tttccggcca tcagtgaacc ggcgaacagc cacgctttcc gtcccataac
     2401 aaccgggcga atggcacgct cgcagatatt gttgtccagg ggcaccgctc cgtcacccag
     2461 gaagcagctc agttcatcgc gccggttcag aatatagtta atcgccttat ttaatggccc
     2521 gtcgggcgga cagcacggct cctgctcttc aagccaggca aacaggtcat tcactatcgg
     2581 ccgggagtac cgttatcgcc actgccgtat tttttccaca gggcgctcac ggatgagttt
     2641 ctcaatgcgg taaagacccg cgatttttct cagctccacg cctgcacggg gctccctgct
     2701 ggccttatac agatttgcga agccgcgccc tgcgtgactc cagcaacagg ctgatgtgat
     2761 gccggggtga ttatccgcca gagatttgta cacgctgtat ccgtcactca ccagcgtacc
     2821 gccacaccag cactgcagcc aggtctcagg gtatctgagg gcccgtccgg tctgcgagtc
     2881 aaaacagacg accggcggac cgcttctttc accgctgacg taagcacaca gccagccgga
     2941 gcaggattta ccgcctttcc gggtatcaag gagcctcagg gacgtttcat ctgcatgaat
     3001 aacatcccgt gtcagaagct catggcgtag cagtttcgcc agcggggcca gtgcagctcc
     3061 ggcaacaccg accatatccg ccattgtact gacggggagc tccacaccca ttcgggagaa
     3121 aatgtgctgc tggcgataca gg
//
LOCUS       DADMGC010000239         3092 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.242, whole genome shotgun sequence.
ACCESSION   DADMGC010000239 DADMGC010000000
VERSION     DADMGC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.242"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..103)
                     /locus_tag="JNZ73_004128"
     CDS             complement(<1..103)
                     /locus_tag="JNZ73_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8734178.1"
                     /translation="MTDITANVIVSMPSQLFTMARSFKAVANGKIYIG"
     gene            complement(239..2080)
                     /locus_tag="JNZ73_004129"
     CDS             complement(239..2080)
                     /locus_tag="JNZ73_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HAY8734179.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTREEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDRPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDALHEVLQQTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNMLLRLANTKAGTAAYERALNNAANAIRPILSSQITAEQ"
     gene            complement(2080..>3092)
                     /locus_tag="JNZ73_004130"
     CDS             complement(2080..>3092)
                     /locus_tag="JNZ73_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005067136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8734180.1"
                     /translation="SSPEAMQSWLQNNANELARVGVDPNSVAQMYQQNPSGFGEFVDH
                     LGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQARGQNLSYQSAMTGHN
                     IAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGIETAVNNATASSNSANS
                     MSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQNALVNSQVLKFLPPGP
                     ATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNEFKAEWMSANGNPGQSR
                     NGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEPRNQVGSYTSKSGIQFT
                     VE"
BASE COUNT          703 a          771 c          677 g          941 t
ORIGIN      
        1 ttccgatata aattttgcca ttggctacgg ctttaaaaga acgagccata gtgaagagtt
       61 gcgaaggcat cgatacgatc acattggctg taatgtctgt catttaattt gctccagata
      121 caatgaatcg ccgcagcgtg gctacggtga attttgggca taaaaaaaac cagccgaagc
      181 tggatagttg cgttggttat ctgtcagtag ttatgtgctt atttaattat ttatctatct
      241 attgttctgc tgtaatttgg cttgaaagta tagggcgtat cgcatttgca gcattattta
      301 acgctctttc ataagctgcc gttcctgctt tggtgtttgc caaacgaaga agcatattcc
      361 ttgcggcttt ggactcatac aaacgcatca ttgcaccaaa accagcctca agccccattg
      421 atacaccaag agtcgcagtt gcgccaatcg tccttatcct gttggcttgc gattgccccg
      481 tctgagttac tacatttgcg gtgtctgacc ttgctgtttg ctgtagaact tcatgaagag
      541 catcaagctc tttcatgtgc tttccagaaa aaatagtgtt gtaaatttca ccgcctgact
      601 gagatttcag cttattaact tcagtgatga acttggctgg agagtcaccg gccttttccg
      661 ctattttgct gacgtaagct gcacgcatag catctttccc tttatcatcc agtgcgctcc
      721 agattcgttt cacgtcagat ggttttctgc ttaatacaac ggtatttata agttcaggac
      781 tggcttcact gcttgccttg ttgagcttgt tagcaatgtt tttattaagc accttattat
      841 aaacgtttgc ataatcggaa tttgctttaa ggtattttgc tgcgtctgat gcaccgaggt
      901 ttttagcaac tgcgttacga aggtcttttg acattgcatt ctctaccata ttggtagctg
      961 cttttgcctg gttggggaag accatagcat ctccctgaac attagatcta aatgctgttc
     1021 tgtgctgacg caagagatca aacgtaacat ccaaatcagt tgcagggttt gctaattctt
     1081 cacgtaggtt acgcaaggat gtaagcaggc tttgattggc agacgtccca agccgttcct
     1141 gtcttgcgat cgctgtattc agagcattca tggtatttgt ggtatcaact gcggcattac
     1201 ccattttatt ggtgacgtca ttgataacag cgccagcggc atccttccgc ccccttaacg
     1261 tggtggtaag agatttcacc acatcatcag ggttgtactc accaaaacgg tcaaaataat
     1321 tacttaccag cttactacgc gttgcatatt gctccgctcg ctttgagcct gtcccgagca
     1381 aagccccctc ggcatcctga gttaggccgc gagtgaaagc atttttcggc gggataacat
     1441 cagatgtcat tggtgtcacg cccatcgatt ctgatgtggc aattttcttc gccacttctg
     1501 gcgcaatatc accttttata gccgttattc cacgccctat tccctttgct gctgcggaaa
     1561 gaacaccctg agcggcaagg ttaactccgg catttttagc tgcattttgt gcgaaatcgc
     1621 ctttctgatt tgcggcctct gccagtgatc caatagccat gcttcctgcc gttccaactc
     1681 ctggaactaa atacccacca attgtttcac cggcttgcgc gtaggggtct gtcggtctgt
     1741 ctactggacg ataaacatca tccaaaacct tgggtccacc aagcccctga ctgattgcat
     1801 taatcagact tgcgccgccc tgcaatacgt caaatggtat gtttaccaga ccacgaccag
     1861 cctgttctgc aatttgccct gcactttgac caccagtgag ccaatcgcca gcttgttgca
     1921 tcaatgatgg ttcttcccgt gttggtgcat tattggcctg attaactgtt tgttgctgaa
     1981 cagcctgacc agcaaaatac tcatcaatgg cggtgccaat atcttcggtg ctcgtaccat
     2041 caggaaaggt aaatgtctta ccgtttgcag ttactttcat cattccaccg taaattgaat
     2101 gcctgatttt gaggtatatg atccaacctg attccgtggt tctcctgaag gtgtcgaatc
     2161 ttgtgctggc gctgcgtcag tattcattga catataccgc ttaacggcac tccccaatga
     2221 ttcacctttt ttaacatcca accccaatat ctgaccgcca ttacgcgatt gtccagggtt
     2281 gccattcgcg ctcatccact cggctttaaa ctcattaaac tgcgcgtttc gtcgctcaag
     2341 gtttgccatt gcatcaagcc atcttgcgac cgtctcaggg ttatccatgt cagttggcgc
     2401 accctgtcga acgatctcaa cgtctttatc cgttgctggg ccgggaggta ggaatttaag
     2461 aacctgactg ttaacaaggg cattttggcg aatgcgcaaa tcacgcaatg ttgtatcgct
     2521 tccggtaagt tttgcgaaca tgttctgtgc gttaccgaac aaacctgtcg ttggtttttc
     2581 tgctctgaac tgttgagcaa gcgcactcat agaattggct gagtttgatg atgctgtagc
     2641 attgtttaca gccgtctcga tgcctttttc catgtttact gacagcttag gtgcttcgct
     2701 aatcaactgc tgagcctttt cctgcgcttg ctgcatctta aacccgaact cttgctgatc
     2761 cagagccaag cgttgtgctg cgatattgtg cccagtcatt gctgactgat aggaaaggtt
     2821 ttgccctctc gcctgaagtg cttctccagc ctgattgctg cggattgtct ctgccagcct
     2881 gcctcggtca atttcacgac cagccatctt gtcctgaaca ttgaagtaat caatcggacc
     2941 aagagcagcc attccaaggt gatcaacaaa ctcaccaaat cctgaaggat tctgctgata
     3001 catctgagca acgctgttag ggtcaacacc gacgcgcgcc agctcattgg cgttgttttg
     3061 cagccatgat tgcattgctt ctggagacga gg
//
LOCUS       DADMGC010000240         3049 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.243, whole genome shotgun sequence.
ACCESSION   DADMGC010000240 DADMGC010000000
VERSION     DADMGC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.243"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="JNZ73_004131"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="JNZ73_004131"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HAY8734181.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLLGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="JNZ73_004132"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="JNZ73_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HAY8734182.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3049)
                     /gene="bcsE"
                     /locus_tag="JNZ73_004133"
                     /pseudo
     CDS             complement(1963..3049)
                     /gene="bcsE"
                     /locus_tag="JNZ73_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
BASE COUNT          763 a          786 c          805 g          695 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aagcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgccctta
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctca
//
LOCUS       DADMGC010000241         3038 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.244, whole genome shotgun sequence.
ACCESSION   DADMGC010000241 DADMGC010000000
VERSION     DADMGC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.244"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            53..1084
                     /gene="ynaI"
                     /locus_tag="JNZ73_004134"
     CDS             53..1084
                     /gene="ynaI"
                     /locus_tag="JNZ73_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HAY8734183.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(1135..2748)
                     /locus_tag="JNZ73_004135"
     CDS             complement(1135..2748)
                     /locus_tag="JNZ73_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8734184.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT          791 a          706 c          671 g          870 t
ORIGIN      
        1 cgccaggggt ttccggcatg acgacaatta tctggaggag cctgataaaa caatgatcgc
       61 tgaactgttt acaaataatg cgcttaatct ggtcattatt ttcggtagct gcgcagcatt
      121 gattctgatg agcttttggt ttcgccgtgg aaatcgtaaa agaaaaggat ttttattcca
      181 tgcggtgcaa tttttaatct acaccataat tatcagtgct gttggtagca tcattaatta
      241 tgttatagaa aactataaac tcaaatttat caccccaggc gttatcgatt ttatctgtac
      301 gtccctgatt gcggttattc tgacgattaa gctatttctg ctgattaatc agtttgaaaa
      361 acagcagatt aaaaaaggtc gcgatatcac cagtgcgcgg attatgtcgc gcattatcaa
      421 aatcaccatt attgtagtgc ttgttctgct ttatggcgaa catttcggca tgagcctttc
      481 tggcttgctg acctttggtg gtattggtgg tctggctgtc ggtatggccg gtaaagatat
      541 tctgagtaat ttcttttccg ggatcatgct ctatttcgac cgtcctttca gtattggcga
      601 ctggatccgt tcaccggaca gaaatatcga aggtacagta gcggaaattg gctggcgaat
      661 taccaaaatt acgacctttg ataatcgtcc attgtacgta ccgaactcgc tgttttcgtc
      721 gatcagcgta gaaaacccag gacgaatgac caaccgccgc attaccacga ccattggttt
      781 acgttatgag gatgcggcaa aagtgggcgt tattgtcgaa gccgtacgtg agatgctgaa
      841 aaatcacccg gccatcgacc agcgacaaac cttactggtt tatttcaacc agtttgctga
      901 ctcgtcattg aatattatgg tttattgctt taccaaaacc acggtatggg ctgagtggct
      961 tgctgcacag caagacgttt atttgaagat tatcgatatt gtacagtcac acggcgcgga
     1021 ctttgccttc cccagccaga cgctgtatat ggataacatt acaccgccgg aacagggtcg
     1081 ctaaatcaga catcactggc gttatccaca agggtaacgc cagttcccat cacatcaatg
     1141 cttcacaata tacatagtcc gactgtacgc tacatcttca gggttattaa tgggataacc
     1201 tttcagccac ggtttgatta atcgtccatt ggtatattga taaattggtg caatcggtgc
     1261 ttgctccatg aggatttttt ctgccgcgtt gtaatcggca ttacgcgctt taacggtatt
     1321 ttccgtcgat gcctgggcca gaactttgtc gtatgccgga ttgttaaagc gtgaaatatt
     1381 tcctgaatgc attgacgtta ataatgtcag gaaagtggag ggttcattat aatcccccac
     1441 ccatgaggcg cggataacat caaaattgcc ggtgttacgg ctatcgatat aggttttcca
     1501 ttcctgattt tgcaatttaa catctacgcc aaggttcttt ttccacatcg atgctacagc
     1561 aatcgcaatt ttttgatggt tttctgaagt gttatacaaa agcgtcagct tcagcggttt
     1621 ttgcggacca taaccagctg cgctcaacaa agtttttgcc tgcgcattca gttcttcctg
     1681 actcatttgt tcaaacggcg aaggttccgg cgtaaatccg gcggtaacat ctggtataaa
     1741 atgccacgct ggcttttcgc ccgtccccaa cactttttcg gtcatcaggc ggcgatctat
     1801 cgtcatactt aatgccaggc gaacgcgctg atctgccgtc ggcccttttt gcgtgttaaa
     1861 ggcataataa taggtcccga gctgcggtgg cgtataaacc tgccccggaa tatccttcaa
     1921 cagcttctga tacatatttt tcgggaagga ttcggtgata tcaatatccc ccgcgaggta
     1981 acgcttagtg gctgcggatt cctgattaat tggcaggaag gtcacttttt gcagcaccgt
     2041 tttggcgtta tcccaataat gggtattcgg taccacgacc agtttttcat tgactacgcg
     2101 ctctttaaga acataagcgc cattgccgat cagatttccg ggtttcgtcc actctttacc
     2161 gctttctacg ttggcttttt gcaccgggaa gaaggcaaag ttagcggtta aattcacaaa
     2221 ccacggcaac ggtttatcaa gctgaatttt caaagtatgg gcatcaactg cggtgacgcc
     2281 aagctgatca ggcgtagctt taccatcaat aatcgcctgt gcgttgttga ttcccgccag
     2341 cgcggcaaac catgcaaatg gcgacaatgt ttttgggtcc accagacgtt gccagctgta
     2401 gacaaaatct tgcgccgtta ccggtgtgcc atccgcccat tttgcgttat cgcgcagggt
     2461 aaaagtccag atacggttgt cattactttt ccattgagtc gcgacgccgg ggacaatctc
     2521 ccctttttcg ttctgattca ccagaccttc aaacagatcg cgaatgacct gaatctctgg
     2581 caggcccacg gctttagcgg gatccagcga cgcaggctca tctttaatgt ggcgcaccag
     2641 ctcctgcttc tctgccagta ctgtgccgct cggaacttct gcagcatacg aaagagaaat
     2701 gctgctgacc aacagcgcac aacacgtaac tgaaacagag tgcttcataa gtttccctct
     2761 aaccgacatt aaaattgatg cgtaattatt tgtttcgcct taaagaaatg caaacaactt
     2821 ccatcaaaaa agtgacaaag gaagcatcaa atgttttaag tatcaatata tcaaatcaga
     2881 tttagtgtaa ataatagacg ccaaaatgcg gcaaagcaca tagaacaccg aacactacgt
     2941 tcgggcttcg cacgttcaaa ccagttttcg gtatcataat cagcatctgt cgccattgta
     3001 cgaagaataa aaaataacca aaacgttgaa aggaactg
//
LOCUS       DADMGC010000242         2964 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.245, whole genome shotgun sequence.
ACCESSION   DADMGC010000242 DADMGC010000000
VERSION     DADMGC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.245"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            144..2012
                     /gene="kup"
                     /locus_tag="JNZ73_004136"
     CDS             144..2012
                     /gene="kup"
                     /locus_tag="JNZ73_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HAY8734185.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2179..2598
                     /gene="rbsD"
                     /locus_tag="JNZ73_004137"
     CDS             2179..2598
                     /gene="rbsD"
                     /locus_tag="JNZ73_004137"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAY8734186.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2606..>2964
                     /locus_tag="JNZ73_004138"
     CDS             2606..>2964
                     /locus_tag="JNZ73_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY8734187.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYV"
BASE COUNT          661 a          703 c          782 g          818 t
ORIGIN      
        1 aaaggggggg ggctttttct ttgagccagg ttaatgtggc cgcatttagg agtacgattt
       61 tgccgttaat cgtgcatact gtgcgctttt ttgtgggcca agggactaag cacacatttc
      121 atatttcaac gaaagactag tctatgagca ctgataataa gcaatcattg cccgcgatta
      181 ccctcgcggc gattggagtt gtctacggcg atattggtac cagcccgtta tatacacttc
      241 gtgaatgttt gtccggccag tttggttttg gcgttgaacg cgatgccgtg tttggctttt
      301 tatcgctgat cttctggctg ctaatctttg tggtttccat taaatatctc accttcgtga
      361 tgcgggcaga taacgccggt gaggggggga tcctgacgtt gatgtcgctt gccgggcgta
      421 atacgtcggc gcgaaccaca tcaatgctgg tgattatggg gctaatcggc ggcagctttt
      481 tctatggtga agtcgtcata acacccgcta tttcggtgat gtcagccatt gaaggtctgg
      541 aaatcgtcgc cccgcagctg gatacctgga tagttcccct ctcaattatc gttctcactt
      601 tattatttat gattcaaaag catggcacgg ctatggtcgg taagctgttt gcgccgatca
      661 tgctgacctg gtttttgatt ctggcaggac tggggttacg tagcattatt gctaacccgg
      721 aagtgctgca tgcgctgaat ccgatgtggg cggtgcattt cttccttgaa tacaaaacgg
      781 tttcttttat tgcattaggg gcagtggtgc tgtcgattac gggggtcgag gcgctgtatg
      841 ctgatatggg gcactttggt aagttcccta ttcgcctggc gtggttcacc gtcgtattac
      901 cttccttgac ccttaattac ttcggtcagg gagcgttgtt gttaaagaac ccggaagcga
      961 ttaagaaccc gttcttcctg ttggcaccgg actgggcgct gatcccactg ctgatcatcg
     1021 ccgcactggc gacggtaatt gcctcgcagg cggttatctc tggcgtcttc tcattgacgc
     1081 gtcaggcggt acgtctggga tatttgtcgc cgatgcgcat tattcacacc tccgaaatgg
     1141 agtcagggca aatctatatt ccgtttgtga actggatgct ctatgtcgcg gtcgtgattg
     1201 tgattgtcag ctttgagcac tccagcaacc tggcggcggc gtacgggatt gcggtgaccg
     1261 gaaccatggt actgacgtct attctctcga ctaccgtggc acgtcagaac tggcactgga
     1321 ataagtattt tgttgcgctg atcctgattg ctttcctttg tgtcgatatt ccattgttca
     1381 ccgctaacct cgataaactg ctctccggcg gctggttgcc attgagcctc ggtactgtga
     1441 tgtttatcgt gatgaccacc tggaagagcg agcgtttccg cttgctgcgg cggatgcatg
     1501 aacatggtaa ctctctggaa gcgatgattg cttcgctgga gaaatcaccg cctgttcgcg
     1561 tgcccgggac cgcggtgtat atgtcgcgtg caatcaacgt cattcccttt gcgctgatgc
     1621 ataaccttaa acataacaag gtattgcatg agcgggtgat tttgttaact ctgcgcaccg
     1681 aagacgcgcc atatgtccat aacgtccgtc gggtacagat tgaacaactg tcgcccactt
     1741 tctggcgcgt ggtggcaagt tatggttggc gagaaacgcc aaacgtggaa gaagttttcc
     1801 accgctgcgg tctggaagga ttaagttgcc ggatgatgga aacctccttc tttatgtcgc
     1861 atgagtcgtt gatcctcggc aaacgcccgt ggtatttgcg tctgcgcggc aagctgtact
     1921 tgctgctgca acgtaatgcg ctgcgtgcac cagatcaatt tgaaatcccg ccaaacaggg
     1981 ttatcgaact gggtactcag gtcgaaatct aacgccagac gcctcctttc ttcataaggg
     2041 ggcgtttttg ttttcatggt taatcaccat gtaaaacgtt tcgaggttga tcacatttcc
     2101 gtaacgtcac gatggttttc ccaactcagt caggattaag ctgcggatca gcgaaacgtt
     2161 tcgctgatgg agaaaaaaat gaaaaaaggc accgttctta attctgatat ttcatcggtg
     2221 atctcccgtc tgggacatac cgatacgctg gtggtgtgtg atgctggttt acccatcccc
     2281 aaaagtacaa cgcgtatcga tatggcatta acccaaggtg taccttcttt tatgcaggtg
     2341 ctgggcgtcg tcacaaatga aatgcaggtc gaggctgcca ttatcgcgga agagatcaaa
     2401 caacataatc cgcaactcca cgaaacgttg ctcactcacc ttgagcagct gcaaaaacac
     2461 cagggaaata ccattgaaat tcgttacacc acgcatgaac aattcaaaca acaaaccgca
     2521 gaaagtcagg cggtaattcg cagcggagaa tgttctccgt atgcgaatat cattctctgt
     2581 gctggcgtga cgttctgagg ccgtcatgga agcattactt cagcttaaag gcatcgataa
     2641 agccttcccg ggtgtaaaag ccctctcggg cgcagcgtta aatgtctatc cgggccgtgt
     2701 gatggcgctg gtgggcgaaa acggcgcggg taaatccacc atgatgaaag tgcttactgg
     2761 tatctatact cgcgatgccg gcacgctttt atggctgggg aaagaaacga catttaccgg
     2821 gccgaaatct tcccaggaag ccgggattgg gattatccat caggaactga acctgatccc
     2881 gcagttgacc attgccgaaa acattttcct cggtcgtgag tttgttaatc gctttggcaa
     2941 aattgactgg aaaaccatgt atgt
//
LOCUS       DADMGC010000243         2923 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.246, whole genome shotgun sequence.
ACCESSION   DADMGC010000243 DADMGC010000000
VERSION     DADMGC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.246"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..546)
                     /locus_tag="JNZ73_004139"
     CDS             complement(<1..546)
                     /locus_tag="JNZ73_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734188.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AVTDKSYLSLNPDKYPNYRFLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDGI"
     gene            complement(1208..2194)
                     /locus_tag="JNZ73_004140"
     CDS             complement(1208..2194)
                     /locus_tag="JNZ73_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734189.1"
                     /translation="MMTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSSTGFTRGTLQQDALNICDNVVRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGADRGVFMMAKRFTALSFGCFRHDGENITV
                     VLSRDGNTQSHDLEILGKVFVVAEAATTVKMGTGSAAAVLPVVKRLSFTKTS"
     gene            complement(2276..2767)
                     /locus_tag="JNZ73_004141"
     CDS             complement(2276..2767)
                     /locus_tag="JNZ73_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734190.1"
                     /translation="MLFNNQDWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRVEAERIAKELASQKE
                     RTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
BASE COUNT          766 a          682 c          721 g          754 t
ORIGIN      
        1 aattccatcc agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg
       61 cgtagacata taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg
      121 gtctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
      181 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
      241 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgaatttc
      301 gtctttggta tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaaaacg
      361 ataattagga tacttatctg gatttaacga taaataactt ttatctgtta ctgcccccct
      421 ggaattaaga atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt
      481 gccgcacgaa aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata
      541 atccatcaat cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg
      601 tattttacct gggttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa
      661 aatggggaag tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag
      721 aaaatcagat acagcaaaaa tggcagattg atcaggtgaa gataaattgg agcgggcgaa
      781 gggagccgag gacaactagc taacttcttg tttcttaaag actgatgaat ccagtggtta
      841 cagtgatggt tacacatgga gaggctaaag gtcaagactt gaagtacgta acattcccac
      901 aaaaggaata gaacacagtc acggactacc cggccctttg gtctgactga gggtcgggct
      961 acggggtaaa tcgtcaggct tattgtatta gatggtctct cacatttttc agtcaaaaac
     1021 tcatcgtact ttaggccaga ccatcagcat gactatcaga gccagcttta gcaggaagac
     1081 cacgagatag actttgtgta ggtccattag gtcctcctct ttccgtaccc atgaactaga
     1141 ttactagtac aaaataaagg ccagacatcc agttacggaa gtctgaccaa taagtcgtta
     1201 ttgttagtta gcttgtctta gtaaagctca ggcgtttgac tacaggcagg acagcagcag
     1261 cgctgccagt ccccatctta acagtcgttg cagcctcagc gaccacgaag accttaccga
     1321 gaatctcaag gtcgtgagac tgggtgttgc catctcggga taagaccaca gtaatattct
     1381 cgccatcgtg acggaagcaa ccgaagctca gagccgtaaa gcgcttagcc atcatgaaga
     1441 cgccacggtc tgcaccgttg tagtaggcga cgtcagtccc cagcatatcg acgatagcgt
     1501 cttcaccacc gaagccctgc ttagcagcca gacgggagag agccttgagg tgtgactcat
     1561 gggcgataat gccgtagtca gaaacctcag aaccgaaaga agactccgtg ttctcgtaca
     1621 gggcatccat gatagcgtca gccttagcgg tcaggctacc agtcagggcc ggaagctcta
     1681 cggtcttcag cagcttagag gactccagaa cgtcaaccat cttagagtta atggtacgga
     1741 ccacgttatc gcagatgttc agagcatcct gctggagcgt cccccgagtg aaaccagtag
     1801 aggacttctg gtcccagagc tttgtctgat gagacacgag gatagcctgt tcaatcggct
     1861 caacggtgat ttcagtacca gacggagcgg tcacgacttc aggtcgctta ccaccacgga
     1921 caggaacgac cacagagcgg tcagccagag tgtgagcaat gattggcgtc ggtaagctgt
     1981 tacgggtcgg caggacttct acagggaacg gaatgttttc ctgagcgtct ttaagtacac
     2041 cgttcagcat agccttaacc gtcttagcta actggagggc atagacctgt gggtcagcgt
     2101 tagtaggcag accttcattg acctgaatgt tgtcggtgtc ggtgatacgt actggctcaa
     2161 taccgaactg gaaagcgtct ttaatcgtca taataatcaa tctcctaaat tagtttaaat
     2221 actgtgagtc ctcaaaactg aggagtccct agcatcgggt cagggactgt gtgttttaca
     2281 ggctgtccat ccagttaccg cctgactcaa ggtcagcgac gatagcggca cgggagcggc
     2341 cagcggaatg ggtagcactg cggaggtttt caccataggt ccggcgttca gctttgacct
     2401 gttcgcggcg ctgtagctct ttctgccatt cgtcttcggt catgaacatt tcgcgggtac
     2461 gctctttctg agacgccagc tctttagcga tacgctcagc ctcgacacgc ttattcatct
     2521 ggcggcgttg gtaggcggtc atcaggtcat tactgaagaa gttaccaatg gtcacgccag
     2581 cttcagccag agcttccagt aactccattg cctcgcggct ggtagacggc aggcctgaca
     2641 ggtagcccgg aatgagggtc ttgatagcga gcgacagcgg atagggctga ccgtccagac
     2701 tcacagtatt ctcgtagaga ttgatgtcgg tgactgacag tttccagtct tggttattga
     2761 ataacatagt aattactcct cttgagagtt gagactatcg gtcgaccaca ggcgtgttca
     2821 gtcctgcggc gtctccccgc atcggtcatc tcaaaaggtt taaaattagg tattgagtag
     2881 acgcgctact aacggtgtgc tgacgacagc tagcaagcta gcg
//
LOCUS       DADMGC010000244         2870 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.247, whole genome shotgun sequence.
ACCESSION   DADMGC010000244 DADMGC010000000
VERSION     DADMGC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.247"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="JNZ73_004142"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="JNZ73_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HAY8734191.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="JNZ73_004143"
     tRNA            complement(621..695)
                     /locus_tag="JNZ73_004143"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(771..1703)
                     /locus_tag="JNZ73_004144"
     CDS             complement(771..1703)
                     /locus_tag="JNZ73_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HAY8734192.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMVIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1997..2752
                     /gene="mlaA"
                     /locus_tag="JNZ73_004145"
     CDS             1997..2752
                     /gene="mlaA"
                     /locus_tag="JNZ73_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HAY8734193.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPVRNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          695 a          697 c          689 g          789 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagccttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcatgagta tacccgctaa
      721 tggactgcgg ggtaagtacg ctgccgctcg attgcttaaa ccctcgccat ttatgctggg
      781 tttttatcat ttttcttaat gttttccgca cgttctgctt tttggcgtgc ttctgcttta
      841 cgcttgttgc tcatgtcgtt acgaatctgt gcatgactca ttaacgcgaa gataaaggtg
      901 ccgccgcaga tgttccccgc taaagtaggt agtgcgaagg gccagatgaa atcgctccag
      961 tgcagcgtac cgttaaacac cagatagagg atttcaacag aaccgaccac gatatgggtg
     1021 gtgtcaccca gggcaataag ccaggtcatc aatataatca ccacaatctt tgccgcacct
     1081 gctgcaggaa acatccatac catagtggcg atcagccagc cggaaatgat cgcgttggca
     1141 aacatctcgc tgggggtgtt cttcatcaca tccatgccga ttttgacaaa tgcatcgcga
     1201 gtttcttcat tgaagatagg catatattca aatgcccacg cagcaatacc agtcccgaga
     1261 atattaccca gcagcacgac tccccataac cgcataagta agccaacgtt gctcattgtc
     1321 ggtttttgca tgacgggtag taccgcagtc acggtgtttt cggtaaataa ttgctggcgg
     1381 gccattatga cgataataaa accaaaggta taaccgagat tctccagcaa gaagctgccc
     1441 ggcacacctt ccagttcgac atgaaatatc ccttttgcca gtaacgaagc gcccatcgac
     1501 agacccgccg caatggctga ccacagtagc gccattgcgt cgcgttctag ctctttttca
     1561 ccatcctggc ggatatgctc atgaattacc atcgctcggg aggggagtcg gtcttcatct
     1621 atttctattt ttttgccgcg ctctttttct tcgctctcaa cttcaatttc gtcgctgtgt
     1681 tgatcaattt tgtcgttgtc catggtctct tctcttgaat tagcacgtat agctaaagcg
     1741 tagcggcttt tttgctcgca actggcggga gttactctga aaatgtagaa aaggttgcgt
     1801 ttgccttttt ctgtttctat agaatcaagt agcctacagg gcggcgatta ccaggctatg
     1861 atcaaatcag caaatcaggg cgtctggaca tcagttgacg tgctgttaca atcgcccaca
     1921 cctaaacagg cggatacggt atcgttccgt catggatggc aaactgcata agccataaaa
     1981 aaacagggag acatttatga agcttcgcct gtcggcgctt gctctgggaa ctacgcttct
     2041 ggtggggtgt gcgagttccg gtacagatca gcaagggcgt tctgacccgt tagaagggtt
     2101 caaccgcacc atgtacaact tcaacttcaa cgtattagac ccgtatattg ttcgaccggt
     2161 cgctgtcgcc tggcgtgatt atgttccgca accggtgcgt aatggtttga gcaactttac
     2221 tggcaacctt gaagaacctg cggtgatggt taactacttc ttgcagggcg acccttatca
     2281 ggggatggtc cactttaccc gctttttcct gaacaccatt ttggggatgg gcggctttat
     2341 tgatgttgca gggatggcga acccgaaact gcaacggact gaacctcacc gcttcggtag
     2401 tacgcttggt cattatggcg tgggttatgg gccttacgtt cagttaccgt tctacggtag
     2461 cttcacgctg cgtgatgacg gtggtgatat ggcggatggt ctttacccgg ttctttcctg
     2521 gctgacctgg ccgatgtctg tgggtaaatg gacgcttgaa gggatcgaaa cccgcgctca
     2581 gctgctggat tccgatggtc tgctgcgtca gtcgtccgat ccttatatta tggtgcgcga
     2641 agcgtacttc cagcgtcatg atttcatcgc taatggcggc gaactcaaac cgcaggaaaa
     2701 tccgaacgcg caagcgattc aggatgattt aaaagatatc gattctgaat aagaaacaaa
     2761 taaaaaaggt gagtcgcaaa actcaccttt tttgttgtta tccctcaatt accacaagta
     2821 aaaggagata aaccgacaat gacccggacg taatgatgta aaaatactga
//
LOCUS       DADMGC010000245         2796 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.248, whole genome shotgun sequence.
ACCESSION   DADMGC010000245 DADMGC010000000
VERSION     DADMGC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.248"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..389
                     /locus_tag="JNZ73_004146"
     CDS             <1..389
                     /locus_tag="JNZ73_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="required for the translocation of capsular
                     polysaccharide through the outer membrane; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HAY8734194.1"
                     /translation="NDDLKVFVMGEVGKQSTLKMDRSGMTLAEALGNAEGMNQDVADA
                     TGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQLEPYDIVYVTTAPLARWNRV
                     ISLLVPTISGVHDLTETSRWIQTWPN"
     gene            377..823
                     /gene="etp"
                     /locus_tag="JNZ73_004147"
                     /gene_synonym="yccY"
     CDS             377..823
                     /gene="etp"
                     /locus_tag="JNZ73_004147"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HAY8734195.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            843..>2796
                     /gene="etk"
                     /locus_tag="JNZ73_004148"
     CDS             843..>2796
                     /gene="etk"
                     /locus_tag="JNZ73_004148"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HAY8734196.1"
                     /translation="MTTKNMNTPLGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMLPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINTLQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQHQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPM"
BASE COUNT          739 a          661 c          746 g          650 t
ORIGIN      
        1 gcaacgatga tctgaaagtc ttcgtgatgg gtgaagtggg taaacagagc acgctgaaaa
       61 tggaccgcag cggtatgacc ctggcagaag cgctgggtaa cgctgagggt atgaatcagg
      121 atgtcgctga tgcgacgggg atttttgtta ttcgtgcaac gcaaaacaag cagaatggca
      181 aaatcgccaa tatttatcag ctgaatgcga aggacgcatc ggcaatgata ctcggaacgg
      241 aattccagct tgaaccttac gacattgttt atgtcactac cgcgccgctg gctcgttgga
      301 accgtgtgat ttctctgctg gtaccgacga tttccggggt gcatgacctg acagagactt
      361 cacggtggat tcaaacatgg cccaactaaa atttaactca atcctagtgg tttgtaccgg
      421 caacatttgc cgttcgccca ttggcgagcg cttgctgcgt aaacgtctgc cgggcgtaaa
      481 agtcaaatcg gcaggtgttc atggtctggt aaaacaccct gcagatgcga cagcggcaga
      541 tgttgccgct aatcatggcg tatctcttga aggacatgct ggacgtaagc tcactgctga
      601 gatggcacga aattacgatc tgattctggc aatggagtcg gaacatattg ctcaggttac
      661 cgcaattgct cccgaggtcc gcgggaagac aatgctgttt gggcaatggc tggaacagaa
      721 agagatcccg gatccctatc gtaaaagtca ggacgcattt gaacatgtct acggtatgtt
      781 ggagcgcgcc agtcaggaat gggcgaagcg cctcagccgg taatttgagt ttataaaata
      841 cgatgacaac taaaaatatg aatacgccac taggcagcac tcaggaaaat gagatcgatc
      901 tgcttcgtct ggtcggcgag ttatgggatc accgtaagtt tattatcagc gtgaccgcgt
      961 tattcacgct gatcgctgtc gcttactcgc tgttaagcac accaatttat caggcagata
     1021 ctctggtcca ggttgagcaa aaacagggca acgccattct cagcggcctg agcgatatgc
     1081 tccctaactc atcgcccgag tctgcaccgg agatccaact gctgcaatcg cgcatgattc
     1141 tcggtaaaac cattgctgaa ctgaatctgc gcgacatagt tgagcagaag tattttccga
     1201 ttgtgggtcg cggctgggcg agattaacca aagaaaaacc aggtgagctg gcgatcagct
     1261 ggatgcatat tccacaactg aatggtcagg atcagcaact gacactcacg gttggggaaa
     1321 acggccacta tacactggaa ggtgaagagt tcaccgtcaa tggtatggtc ggccagcgtc
     1381 tggaaaaaga tggcgttgcg ctgactatcg cggacattaa ggccaaacca ggaacacagt
     1441 ttgtcctgag ccagcgtacc gaactggaag cgattaacac attgcaggaa acctttaccg
     1501 ttagcgaacg cagtaaagaa agcgggatgc tggaacttac catgactggt gatgatcccc
     1561 agttgattac tcgtattctg aacagcatcg ctaacaacta tttgcaacag aatatcgctc
     1621 gccaggcggc gcaggattca caaagccttg aattcttaca gcaccagtta cctgaagtgc
     1681 gcagcgagct ggaccaggcg gaagaaaaac tcaacgttta tcgccagcag cgcgattcgg
     1741 ttgaccttaa cctggaagcc aaagccgttc ttgagcagat tgtgaacgtt gataatcaac
     1801 tcaatgagct gaccttccgc gaggcagaga tctcccagct gtataagaaa gatcacccaa
     1861 cttatcgtgc gctgctggaa aaacgccaga cgctggagca agaacgcaaa cgcctgaata
     1921 agcgggtatc ggcaatgcct tccacccaac aggaagtgtt gcgtttaagt cgtgacgtag
     1981 aagcgggccg tgcggtatat ctgcaattac ttaaccgcca gcaggagttg agtatttcga
     2041 aatccagtgc cattggtaac gtgcggatta tcgacccggc agtcactcag ccgcagccag
     2101 tgaaaccgaa aaaagcgttg aatgtggtgc ttggttttat tcttggcctg tttatttcgg
     2161 tgagtgccgt gctggcgcgt gcgatgttgc gtcgtggtgt agaagccccg gaacaactgg
     2221 aagagcacgg catcagcgtt tattccacta tcccaatgtc cgagtggctg gataaacgca
     2281 cccgtctgcg taagaaaaat ttattttcta atcagcagcg ccatcgtact aaaaatatcc
     2341 ccttcctggc ggtggataac ccggcggatt ctgctgtgga agccgtacgt gcgctacgaa
     2401 ccagtctgca tttcgctatg atggagactg agaataacat tctgatgatc accggtgcga
     2461 cgccagacag tggtaaaacg tttgtcagtt caactctggc agcggtgatc gcccagtccg
     2521 atcaaaaagt gttatttatt gatgccgact tacgccgtgg ttattcgcat aacctgttta
     2581 ccgtgagtaa tgaacatggc ttgtcggaat atctggcagg taaagatgag ctcaacaaag
     2641 tgatccagca ttttggcaaa ggaggctttg atgtgattac tcgcggtcag gtgccaccta
     2701 acccgtctga actgctgatg cgcgatcgga tgcgtcaatt actggaatgg gcgaacgacc
     2761 attacgatct ggtgattgtc gatacgccgc cgatgc
//
LOCUS       DADMGC010000246         2774 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.249, whole genome shotgun sequence.
ACCESSION   DADMGC010000246 DADMGC010000000
VERSION     DADMGC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.249"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            1..456
                     /locus_tag="JNZ73_004149"
                     /pseudo
     CDS             1..456
                     /locus_tag="JNZ73_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            643..807
                     /locus_tag="JNZ73_004150"
                     /pseudo
     CDS             643..807
                     /locus_tag="JNZ73_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1301..1879
                     /locus_tag="JNZ73_004151"
     CDS             1301..1879
                     /locus_tag="JNZ73_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734197.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1988..2246)
                     /locus_tag="JNZ73_004152"
                     /pseudo
     CDS             complement(1988..2246)
                     /locus_tag="JNZ73_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2230..2774
                     /locus_tag="JNZ73_004153"
                     /pseudo
     CDS             2230..2774
                     /locus_tag="JNZ73_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          861 a          507 c          731 g          675 t
ORIGIN      
        1 tttttcagca gggaaaccga tggcttaact ggctggagaa gcagccggtg cagatgagcc
       61 gcctggaaca ttatgccggt caggatgaaa ttggcctgcg ttacaacagc caccggacaa
      121 aacgagaaga aaaccttgtg atgagtggcg acgagtttat ggaaaggttc tcctggcatg
      181 tggcggataa ggggttccgg atggtgatac ggggtcctga gtccggtgaa gcggcgatta
      241 ctggaagatg tggtgtacgc cataacggag acagtgagaa aaacggcgag gcaaatcaca
      301 aggagaggga tgtatcagca gtgactgaag gttgacccgc tgaagtgcga gctgcgcgga
      361 ggtcagatgc ggtttacggg gctgaagagg ggttaccgtc tggcagagca ggttatgatg
      421 catgagccgc tggctcgaat acggtggtgc ggctgagagt cgcatagggg aagttgcgtc
      481 catgttgagg ggaacggagc aaaaaaccat cagagataca ttgaatcaat caatgtaagc
      541 aatattaagt gtctataccg attacttgat gatcaggttg tgttgaataa acgcgcattc
      601 ttcggtgctt ttttaatctt ttggggattt aaattcctat cgatgaactg tcgatatgaa
      661 aaagcgagca tcatactgac gtcaaacaaa ggggtcgcag actggggaga aatgttcgga
      721 gatcacgtgc tggcaacagc gatcctagat cggctgctac acgctgaata tcaaaggaga
      781 gagttaccgg ttaaaagaga aacgtaaagc tggagtgctg accaaaaaca caacgccaat
      841 cagtgatgat gaaatggtgg aaaacggaca gcatcagtaa cgaaagtatc ttagcaggca
      901 tgaaaatcgt ccgcatcccc taaacggtca aaatgcttct gccgtgaagg agggagaatg
      961 ccatctcatc acttatatct ggcgaatgaa acacagatgg tcggccttgc ccccaaacac
     1021 acggcggtgc aaaatggaat ggcagaaaac ttcgtcaaaa cgatgaaata tgattaaatc
     1081 agtgttatgt tcgaacctga cgtgttaaca gcagtaaagt agtctcgcga aggtgttcaa
     1141 acattataat gaatggcatc ctcatagtgc gcagggttat cgcttgctac ggaaatatct
     1201 gaagcgacgt accagtaatg gggaaaatga tagctggttt ctgaaaatat agaggaaaat
     1261 gtgatatata tatcctgtta atatttaagg agaatataaa atgaaagtct cgtttaagtc
     1321 tttaggctat atttttcatg atatatataa taaaaaacac actatcgatg agttcaacga
     1381 tgtggtaaga aaagcagttt tatcaggtaa aattaacgag ctgaatgcat gtcataaagt
     1441 ggcaattttt cttgccgaaa aagacaatga gataacaaag aaagacaaag ccaagataat
     1501 agatacatta actgaaaact attccattga atttcaacaa ttaatgaata ttagtgagcg
     1561 cacgttaaat tcatctttat atattactcc tggtgaaagt ggttttgtca gttttgttaa
     1621 tcgtgaagga aaaatatgtc atactgcata tgtaaaatca tcagataata gcatggcata
     1681 ttaccatgca aatggtagca gcattgacaa atatattacg gatatgtgcg gccttatttg
     1741 tatgaggcat attgagtcta ctggcattat tttttatatg cttgatgaaa aagttttatc
     1801 tgccattgct gaatttatga atgaaaaagg atggcgggct gcgttttgtt cagcaaagaa
     1861 cttatataaa tgtgtttaat tttagaaaaa ctatttggta aaatccaaca agagtcgaga
     1921 ggttcgcgtt gcttcagcag ggcctcagcc tgttcaacca gactcagttg caggtcgtga
     1981 cgcagattta gtggtggtgg ttgattgtca gataccagcc gatgatcttg tagtgcatat
     2041 catcagactt tgagaagcat atggttttgc gggtaagccg cttaagatgt gtccgcaggt
     2101 tcagattatg tcgctctatc cgctgagtgc atttcttgct tataacatga ctggttgagg
     2161 acaataaagc tcggtatacc ggctcaggca tcggtcatgt agaaggcaat gctaaactgg
     2221 ctcagcaaac taagagcagc gcgcatccac ttacccaatg cggttgagaa aatagccttc
     2281 gatcgctttc atgtggcgaa acaaccgggt gaggtggtgg ataaaacccg ccagaatgaa
     2341 catccgcatc ttcctattga aagtcgtcgt cagacaaagg gaactcgctt cctgtggcag
     2401 cacagcgata aatggatgac cgaatccagg caggaaaagc tgatatggct gcgtgcgcag
     2461 atgaagctga cgagcctgtg ctgggcgctg aaagaactgg caaaggatat ctggagcaga
     2521 ccatggagcg aggaaagacg caacgactgg cagagatggc tgcggccaac agtgacgtcc
     2581 ccatgatgaa gaatgtggct aaaacgatag ggaaaaggtt gtacggtatc ctgaacgcca
     2641 tgcgccatgg cgtatcaaac ggaaatgcgg aagcactgaa cagcaagatc aggctgctga
     2701 gaataaaagc caagggctac cgcaaccggg agcgctttaa gctgggagtg atgttccact
     2761 atgggaaact gaat
//
LOCUS       DADMGC010000247         2731 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.250, whole genome shotgun sequence.
ACCESSION   DADMGC010000247 DADMGC010000000
VERSION     DADMGC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.250"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..1016)
                     /locus_tag="JNZ73_004154"
     CDS             complement(<1..1016)
                     /locus_tag="JNZ73_004154"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8734198.1"
                     /translation="MNSEIKVMSNRDPSIDTLRVTACIFVIISHYGYIFSQTRLSFIP
                     SFYGGVLGQVGVSLFFFISGYLAFISLDRKNTIDYFLDKGVRILTPYIITYFFISLLL
                     ILLSYLNKEIINTVPLVSIMTTGGQYTRIIPVFFALDGILNAHFSYDFLFLTGEWFIG
                     CVIMLYVISPIVFHVMNTFTFKRNIFFLFVITITTFPISNISSSFLNFPFWFFACRLP
                     EFMVGMLAFKYKNKLQKNTTVILSVVICILTVLLIGPNEFKTNIVNHQLSIIPLMCIS
                     IIIYKAALIINKKFNLYFFNSYAKCIYISMLIQHVVIYRLTSIIEINNASVFGYLFIL
                     LMVF"
     gene            complement(1134..1223)
                     /locus_tag="JNZ73_004155"
     CDS             complement(1134..1223)
                     /locus_tag="JNZ73_004155"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734199.1"
                     /translation="MRQHLTRKGQKSLSLSTSVEQHDKAISTI"
     gene            complement(1659..1820)
                     /locus_tag="JNZ73_004156"
     CDS             complement(1659..1820)
                     /locus_tag="JNZ73_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY8734200.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSL
                     EVMRLLRLN"
     gene            complement(2472..2663)
                     /locus_tag="JNZ73_004157"
     CDS             complement(2472..2663)
                     /locus_tag="JNZ73_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY8734201.1"
                     /translation="MTKQEKTALNMARFIRRQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
BASE COUNT          802 a          553 c          596 g          780 t
ORIGIN      
        1 aaaaacacca ttaatagtat aaataaatac ccaaaaacag aagcgttatt aatttcaatt
       61 attgatgtta atctgtatat tactacatgc tgaatcaaca tggatatgta gatgcattta
      121 gcatatgagt tgaaaaaata gaggttaaat ttcttattaa ttattaatgc tgctttgtaa
      181 atgataatgc ttatgcacat taaaggtatt attgataact ggtgattgac tatgtttgtt
      241 ttgaactcat taggccctat taataatact gtcaatatac atataacaac cgatagaata
      301 actgtggtgt ttttttgtaa tttatttttg tatttaaatg caagcatccc aaccataaac
      361 tcaggaagtc ggcaagcaaa aaaccaaaat gggaagttta aaaatgatga tgagatattt
      421 gatatcggga atgttgtaat tgttatcaca aataaaaaaa atatgttacg tttaaatgta
      481 aaggtattca taacgtgaaa aactatcggt gatatcacgt acagcataat aacgcaccca
      541 ataaaccact cgcctgtaag gaaaagaaaa tcataagaga aatgggcgtt taatatacca
      601 tcaagggcaa aaaatactgg aattattcta gtgtactggc ctcctgttgt cattattgat
      661 acgagaggga ctgtattaat tatctcttta tttaaataag acaataaaat taagagtaaa
      721 gagataaaaa agtaagtgat aatataagga gtaaggatcc ttactccttt gtcaaggaaa
      781 taatcaatgg tattctttct atctagtgaa ataaatgcca aatatccaga tataaaaaaa
      841 aacaagctca cacctacctg gccaagaacc cctccataaa atgatggtat gaaagatagc
      901 ctggtttggc taaatatgta gccataatga gatataataa caaaaatgca ggctgttact
      961 cgcaatgtat caattgacgg atctctatta gacataacct ttatttcgct gttcaaatta
     1021 tcgcctttag ttgatgatgt aaaattaacg ctataaataa gctacacatt ctagagcagc
     1081 gaaaggatga ttcaagtatt tttaataata aaacgcattg ataatgttta tgttcagatg
     1141 gtgctgatag ctttgtcatg ctgttccact gatgttgata acgacagaga cttttgtcct
     1201 tttcttgtta gatgctgcct cagattcgga ttatggcgtt aaaggggttt gacgctcaac
     1261 cgaacggttt cacacgttaa gagctttttg tctgataaat caaaatataa tggacattga
     1321 aaaacgccct caaaacggtg tctttttaat cgaagattgg cacaaaacgg acaccaccgt
     1381 ccattagtcg attttttaga cggcttttct cgcctaacgt gagttttcgt tcggttgagc
     1441 atcagacccc ttttatattg tagatttcta tttttagaca gttggaacag gacaattaac
     1501 aacagatcta cttgagatca tttgcttgtc gtcgtagtct ctttcccaga aacgaaaaaa
     1561 ccgccctgca aggcggtttc tcgaaggttc tcagagcgac caactctttg aaccaaggta
     1621 actggcttat gaggagcgca gtaaccaaat ctgtcctttc agtttagcct taacaggcgc
     1681 ataacttcaa gactaactcc tctaaatccg ttaccaatgg ctaccgccac tggcgatttg
     1741 tcgtgtcttt ccgggttgga ctcaagttga tagttaccgg aacgggcgtg gtagtcggac
     1801 tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt
     1861 gtcaggcgtg gaatgagata aacgcggcca taacagcgga atgacaccgg taaaccgaaa
     1921 ggcaggaaca ggagagcgca cgagggagcc accaggggga aacgcctggt atctttatag
     1981 tcctgtcggg tttcgccacc tctgacttga gcgtcggttt ttgtgatgct cgtcaggggg
     2041 gcggagccta tggaaaaacg ccatcggcgc ggccttcttc ctgtttctgg tcttttgctc
     2101 acatgttctt tccggcgtta tcccctgatt ctgtggataa cagcatttcg tttttgagtg
     2161 agtagatacc gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc
     2221 ggaaaagcgc ctggacgtgc attttctcct tacgcatctg tgcggcattt cacacccggt
     2281 atggcgcact tttcatacaa tctgcactga tgccgcatgg ttaagcccgt atacactccg
     2341 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgctaaaacc tgctgacgcg
     2401 ccctgacggg cttgtctgct cccggcatcc gctcacagac aagctgtgac cgtctccggg
     2461 agctgcatat gtcagaggtt ttcaccgtca tccccgaaac gtgcgaggca gctgcggtaa
     2521 agctcatcgg cgtggtcgtg aagcgattca cagatatcag cctgttcatc cgcgtccagt
     2581 tcgttgagct tctccagcag cgttaatgtc tgccttctga taaagcgggc catgttaagg
     2641 gcggtttttt cctgtttagt cactgatacc tccgtgtaag ggggatttct gttcatgagg
     2701 taatgatacc gatgaaacgc gagaggatgc t
//
LOCUS       DADMGC010000248         2730 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.251, whole genome shotgun sequence.
ACCESSION   DADMGC010000248 DADMGC010000000
VERSION     DADMGC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.251"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..195
                     /locus_tag="JNZ73_004158"
     CDS             <1..195
                     /locus_tag="JNZ73_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8734202.1"
                     /translation="APMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRH
                     SRLGNISPAAFREKYHQMAA"
     gene            complement(240..887)
                     /gene="yidZ"
                     /locus_tag="JNZ73_004159"
                     /pseudo
     CDS             complement(240..887)
                     /gene="yidZ"
                     /locus_tag="JNZ73_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
     gene            complement(862..2037)
                     /gene="mdtL"
                     /locus_tag="JNZ73_004160"
     CDS             complement(862..2037)
                     /gene="mdtL"
                     /locus_tag="JNZ73_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HAY8734203.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(2169..>2730)
                     /locus_tag="JNZ73_004161"
     CDS             complement(2169..>2730)
                     /locus_tag="JNZ73_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAY8734204.1"
                     /translation="VVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKS
                     FLGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTF
                     LPPALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIP
                     AIVILFGITVILCWFGNVFNVLPKFG"
BASE COUNT          783 a          721 c          691 g          535 t
ORIGIN      
        1 gctccgatgg aaagcttctg gggaacgctg aaaaatgaga gcctgagcca ctatcgtttt
       61 aataaccggg atgaagccat ctcagtaata cgggaataca ttgagatttt ctacaatcgt
      121 cagcgtcgtc actctcgtct ggggaatatc tccccggcag ccttcaggga aaaatatcat
      181 cagatggctg cttaaaaaaa gaacaaatgg tagtgtccgc tattgccagt acacctcacc
      241 caggtgtcgc tctgttccca gcaaatgctg atatgcggat aacgtaaaaa ggtgtccaga
      301 ttccacgttt catgcagtgc cggatgatct ttgcgtaacc agacgcaggg tacatcacta
      361 aacagcactt cataatcaat ggctaacggt agcgagctta acagctcccg cgagcgagga
      421 tggctttcgc gaccggaaaa accaatatcc acttcaccac gagtaatggc atctaaggaa
      481 tcgtaatccc agttacgtag tttgatggtc gcctgcgggt aacgttggta gatccgtttc
      541 gacagcgcat taagcatgat catcatcagc ggcgattccg ccgccaactc aaacttcagg
      601 ccgcgcggtg tctggtggtg cggtttatcc agcagcagat tgctcatttg catccattcc
      661 gccagatttt gctccatgct gaccattagc tgtgtgggcg acagccccag cggtgagttc
      721 acaaagagcg ggtcgtcaaa ccacgctctt aactttgcca gcgacttact caccgctgaa
      781 ggggtcacgt tcatccgctt cgccgctttg gtcacgctgc gctcctgcat cagcagttgc
      841 agacagagca gcagattgag atcaagcgtg gtgatggatt tcttcatgag cggcaacggg
      901 gcgtccaggc gcgacgaaca taatcagcaa caggctcact atgctacagg caatcagaat
      961 cccgatcagc atattccatg cgccgatacc aaccaccgcg gccagccaaa tccacagtga
     1021 cgaaccgcaa acctgcgcaa tacctaaggt cgagctggct acgcccgcgc gtaatgaaaa
     1081 tggtcctaac gcctgactca tcgctacgcc aaaacctacc gagaaaccgg cgcaaatcag
     1141 cgtgatacca aacagagaaa ccgcatggga aggtgaaacg gcaagggtga tccccgccgc
     1201 aaggaataac acctgcgagg tgatcatcaa cgtacgtggc ttaaaaattc ccagcgcaaa
     1261 tggcgtcgag aatgaaaagg tcatgctgac gccagcggtc agcgccataa tggtggcgta
     1321 ttctccgcgc tcaaacccca tgatttccat cagcaatacc ggcgatgtat tgacgaaagt
     1381 gaggatcacc gaaacgctga gggtggtgat aacaacacgg ctgaggaaaa aacgattaag
     1441 cagcgactcg ctattttctc gtggtttgtc cgaagcagcg ggggacgctg ggcgcgtttc
     1501 ttttaaaata aacaaagaca acatcagtac cgcgatgccc atcattgcca tcgcccagaa
     1561 cagactctgc cacgggaatt taagcataat cagatgtccg agcactggcg ctaacaccgg
     1621 aatgatgcag gtaataccgt tgagtaatga cagcacttta gcccgacgtc gatcatccag
     1681 cgtgtcgcgc aaaatggcga acgccaccac gtaacaacag cctgcgccca acccctgtag
     1741 aaatcggcct gcaagaaata acgtgctggt ttcagccagt gaacagaaca ccgaggcaat
     1801 aataaatagc gccgcgccgg gtatggcgac cggctttctc cctgaacgat cggccacttt
     1861 accggcaaat agcatcgcag ctgccatccc cgccagatat acggagaacg caatatgcaa
     1921 ctgcgcttcg ctggcattga gatcggcggc gatgcgcggt aaaccaacga ggtacatatc
     1981 aatcccggcg ggataaagta aaaccagggc aaaactacaa atcaaaaagc gggacatggg
     2041 ctaaagcgtc ctgatagtaa ggtgacgcaa agcatacgtg gtgaaggagg ataaggcgag
     2101 ttgccatttg gacataagtg atttcctgag aggcaagaag ccagcgaatg gctggcttct
     2161 tgaaggattt agccaaattt aggtaacacg ttaaagacgt tgccgaacca gcacaaaatc
     2221 acggttatac caaagagaat gacaatcgcc ggaataagat tgccgcccca gaccgtgaac
     2281 atctgattgg gaaacttctt gcgagctttg attgcaagca ctgcgggaat aatgaccgcc
     2341 cagatggtgg cgcacagccc ggcaccgccg atcccgtaaa taaagccgtt cgggaagatc
     2401 agatacaaca acgcaggtgg caggaaggtt aacagcacgg ttttgaaacg cccgccgtgg
     2461 gagttatcaa tcttaaacag gtccgccaga taatcgaaca accccagcgt gacaccaaag
     2521 aacgaactgg caacagctaa gttagagaac accagcaggc aaaactcgat aatgccgtgc
     2581 tgtttggtgc cgaggaacga tttcaccagc gaatcaacgt tgccgcctga ggagattatc
     2641 gccttaaagc tttcgcgcgg aatattcccc atcgtgcaat agagccagaa gagataaatc
     2701 accagcgcca gcagcgaacc aaataccacg
//
LOCUS       DADMGC010000249         2669 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.252, whole genome shotgun sequence.
ACCESSION   DADMGC010000249 DADMGC010000000
VERSION     DADMGC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.252"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..398)
                     /locus_tag="JNZ73_004162"
     CDS             complement(<1..398)
                     /locus_tag="JNZ73_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734205.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSD"
     gene            complement(950..1666)
                     /locus_tag="JNZ73_004163"
     CDS             complement(950..1666)
                     /locus_tag="JNZ73_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734206.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGVLFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVRKHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSYKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            complement(2250..2669)
                     /locus_tag="JNZ73_004164"
                     /pseudo
     CDS             complement(2250..2669)
                     /locus_tag="JNZ73_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847259.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          812 a          590 c          505 g          762 t
ORIGIN      
        1 tcatcgctta cagccactac aggacgtttc cgtatcctgt gaattatcat cccacgtgga
       61 ctgcatatat ttttaataca cacaacccct cttccagcca catgcagtgt cgaggtggtc
      121 ccgcagattc cgtatacccg acaccctgca cgggttccaa gtatctgtac tcccggtggt
      181 ttatcagagt aatagtagtc actgttacat tcgacccagc tctttccccc ggggggatgc
      241 gggaccactc ggaaatacac cccatatggt ccgtgcacat taatctccgc atagcaatgt
      301 tctgttgaat tacgtggcat agccagtaaa gtctcttttg cagcagcggg taccgcatac
      361 cacatacaca ttagtgcaac tatcagcgca gtattcattt tcattgtcac tttccgttac
      421 tggtgctgtc cgtcacagca cctcatgtcc tgcggacagg gcaagctgac cggccataac
      481 aactttcagt tccatcaggg aagtggaagc tagacgccgg acaatcaaat cccgccggcg
      541 gtacaccttc ttcgcagcag agttgttcag tcaacatgaa agtgacagac agtgtcacca
      601 cgatgcagca tcagtatgac ggtgaatcgc cggtcgtagt ttttgttctt tgttttatag
      661 ggagctttct gcatcaggca tcgttcatca cggggtattg gtgcaatgct cggcatcact
      721 cagtccggct ggtgatttgt gatgtttggc aatttatctg attacataat ccggactgag
      781 tcccccctga gtgttttact gttccgagca cttatttagt aactttcttg catagttgtt
      841 tctggcttta attgtgctgc cctaaggttt gtttctcagt atgacagctc aatacatata
      901 cttcgaacgc attatatatt catttaattc actttgtggg agaggattat tactttctga
      961 cataaacatc attgatttca taaaatcccg actgttctgt attaaatatt aagaatctgt
     1021 tactgtcatc tttatatgaa ctagacaaaa cgagccgtga atcataacga gcaagaacat
     1081 aataccaact attattataa caaagcatct gatattcttt ttttaattga ggtttatacc
     1141 agccaacaag aaaggaaaaa agccaaaaat aaaccaaaaa accagccata attaacttaa
     1201 tgcggtgttt tctaacaaaa gggatatctg aaaaacatga tacagaaaag atccgtccat
     1261 atatacgaat tattattgaa ataaccaatg aactaataat gcatgaaagt gataatggaa
     1321 gtatgaaata ttgtttgaga attaaaagtt cgaatattac agggaaaaac agtagggtaa
     1381 tagcaaaaaa tagtctaaaa aaactcaagt cttgtatttt acttcttttc tttataagaa
     1441 gaaaaaataa aacaccaatc ccccaaccgg taaaaagtat acccattact atgatagcat
     1501 agagtaaact tctggcaaca tcatccaaac ctgcaccaac aacccaccat ggaaatccat
     1561 agtaaaaaga ggttccccag ccataagaat aagcactgac ccaccctaca catcccatat
     1621 aggcgatgaa taatgaagaa tttctaagca atgaattgtc attcataaga aaccaactga
     1681 taatgtaaaa aatattcgag ataaaagttg atcttttaat ttttatgcta ggaaattaac
     1741 aataatatca ttgttaatgc atatttatca tatcaaaata ctctgcagaa tgctactatc
     1801 aaaaccagac aagtgaacaa aacttggtca acatttagta acgctagttg gtaatgtctt
     1861 caatcgatag aagtttctgg taatctactg gtattcaaac agttagggct catacagctt
     1921 gctggacctt ccccataacc gcccgaacgt gaaggtctga tgtgttgcaa gagagccgga
     1981 tgcgggaaat ctgcaagtcc ggttcgatga gagggacgtt gtttttactt gttgttgatg
     2041 aagactttca ttattgaaca gaatgataat attgtctaaa caaaacatta aatctcataa
     2101 ttctttgtag aaaacgcttt tcttgaaatt aaaaataagt agaatcagca gtctcattat
     2161 aataacagac aatgtcatgc tctcgcccgt tccggtaata aacggagcgg gctatctttt
     2221 tggaatattt atggcccaca atccagtttt cataaatacg attcattcca ttcttcgttt
     2281 tttctgagca ttgcgttcat aatggtcagc aacttacgca tacaggcaac cagtgccact
     2341 tttttggcgt ttccggctgc aagtaggcgc atgtaaaacg cctttatcac cagattaaac
     2401 cgggggcctc aagcgccgcc atgtacagag ctgttcttcc ccagcacaat caccgaaaat
     2461 ggttcgtcga ccgcgcagag ttcccgagtc ccggtttacc tgagcaacac cgacaagtgc
     2521 gctgatttca cgtctcgaga ggctgctcga ttcaggcatt tccgccagca atgctgcggc
     2581 agtcatggct ctaacacctc agacgttcag taagctcttt aaagtaactc tgaatgtgcc
     2641 tgttcatatc tgggtcttcc ccgatcatg
//
LOCUS       DADMGC010000250         2503 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.253, whole genome shotgun sequence.
ACCESSION   DADMGC010000250 DADMGC010000000
VERSION     DADMGC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.253"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            155..1108
                     /locus_tag="JNZ73_004165"
                     /pseudo
     CDS             155..1108
                     /locus_tag="JNZ73_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1149..2144)
                     /gene="pgl"
                     /locus_tag="JNZ73_004166"
     CDS             complement(1149..2144)
                     /gene="pgl"
                     /locus_tag="JNZ73_004166"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HAY8734207.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVASYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTATLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
BASE COUNT          691 a          614 c          562 g          636 t
ORIGIN      
        1 tattgcgata tggttacatt aaggacaaag catctctaat gactttagct caacgattaa
       61 taacattttt tcattaattt aagttggcta aatgcagtta aaatttttaa cgaccagccc
      121 cccaaaatcg tcgcttccat tttccggagt aataatgaag catgaattat caagtatgaa
      181 ggcatttgtc atactggcag agtccagttc atttaacaat gccgctaaat tactcaatat
      241 tacgcaacct gcattaacgc gaagaataaa aaagatggag gaagatttac atattcaact
      301 atttgagcgt acaactcgca aggttacgtt aacaaaagca ggaaaaaggt tgctcccgga
      361 ggcgcgggaa ttaataaaga aattcgatga aacgcttttt aatattcgtg atatgaatgc
      421 ttatcatcgt ggtatggtga cattagcgtg tattccgacc gcagtatttt attttttacc
      481 gctggcaatc ggtaaattta atgagctgta tcccaatatt aaagtgcgga ttctggaaca
      541 aggcacgaat aattgcatgg aatcggtgct gtgtaacgaa tcagactttg gcataaacat
      601 gaacaacgtc acaaattcat ccattgattt taccccactg gtcaacgagc cattcgtgct
      661 ggcctgtcgg cgtgaccatc cattggccaa aaagtagctt gtagaatggc aggagttagt
      721 gggttacaaa atgattggcg tacgttcctc cagcggcaac cgattgttga tagaacaaca
      781 gctagccgac aagccctgga agctggactg gttttacgaa gtgcgtcatc tttcgacgtc
      841 attaggacta gtggaggcag gactggggat ttcggcgctt cctggtctgg caatgcccca
      901 tgcgccctat tcttcgatta ttggtatccc actggtggaa cccgttatac gtcggacatt
      961 ggggattatt cgccgcaaag atgccgtgct ttctccggca gctgaacgct tttttgcctt
     1021 gctgattaat ttgtggactg acgataaaga caatttgtgg accaatatcg tcgaacgcca
     1081 gagacatgcg ttacaggaga taagttaata caccggcgca ggagagactg cgccggttaa
     1141 atcagcggtt agtgtgcgtt aaccaccacc cacattggtc cctgcccgac cgcatagcgg
     1201 cctttttcat gcagtagccc ctgctcgcca acaatttcgt ataccgagat gtggtgagat
     1261 ttttgcccag cggcaatcag atacttgccg ctgtaatcaa cattgaagcc gcgcggctgg
     1321 gtttccgttg gctggaagcc ttctttactc aacacgctgc catcttccga aacgctgaaa
     1381 acggtaatca gagtggcggt acggtcgcag gcgtataaat ggcgaccatc cggggtgata
     1441 tgaatatcag ccgcccaacg ggtgtcggag aagttttccg gcatcatatc cagcgtctgg
     1501 acacattcga tattgccgtg cggatctttc agttcccaga catccactga gctgtttaac
     1561 tcattgacgc aatacgcata ttgttcgttt ggatggaata ccatatgacg cgggccggcc
     1621 ccttcaacgg tagtcacttc cgcagggtcc tgcgccacca gatgaccatc atcgctgacc
     1681 gtaaacaggc aaatgcgatc ttgctttaat gccggaaccc acagcgtacg gttgtccggt
     1741 gagatattgg cggaatggca accgtccagc ccctcgacta catcgacgac gcccactggc
     1801 aggccatctt ccagacgcgt tacgctcacg ttacccgcat tgtaagaagc tacaaagaca
     1861 aactgcccct ggtgatcggt ggaaatatgc gtcggactac ccggcagcac agactctgcg
     1921 gcaaaggtca gtgcgccatc gtccggggca atacgatacg ccaggacgcg aaactcaggg
     1981 cgaacaccaa catagagata acgtttgtcc gggctgacca ccatcggctg cacctgcccc
     2041 ggcacatcga caacctgtgt cagcgtcagt gcgccttcat gattcaggtt ccagacgtga
     2101 atttgctggc tctcagggct ggcgatataa actgtttgct tcatgaacac acctcaaaaa
     2161 tgataaatcc cttatctttc atacatataa caacacttac tggataaaaa acagacaaca
     2221 aaacacgata aaatacaata tcgcacatca attaaatcaa taagttagca ctccatttcg
     2281 aatggcatat tactaaaata ttagtcaccg acacgaattt gacaatgcca cagatatttt
     2341 cgcgactatt cagggagtat gactctcccc gcttttgttt acctgtatca acgaccgtaa
     2401 acactatgcc ataacaactt ctggccaact tcccattcgc tcaacaatca actcacttcc
     2461 ggcacaaata aacgcattac gttgtagatc ggtattctgg tca
//
LOCUS       DADMGC010000251         2484 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.254, whole genome shotgun sequence.
ACCESSION   DADMGC010000251 DADMGC010000000
VERSION     DADMGC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.254"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            289..807
                     /locus_tag="JNZ73_004167"
     CDS             289..807
                     /locus_tag="JNZ73_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAY8734208.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVI
                     SFQGKEWVHLKE"
     gene            814..1047
                     /locus_tag="JNZ73_004168"
     CDS             814..1047
                     /locus_tag="JNZ73_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbeD"
                     /protein_id="HAY8734209.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
     gene            complement(1349..1816)
                     /locus_tag="JNZ73_004169"
     CDS             complement(1349..1816)
                     /locus_tag="JNZ73_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734210.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQQLQIQEVCNSTLWETAKQQLLQESDGRYFAINSKHL
                     DFPGSGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFSHNLT"
     gene            complement(2144..>2484)
                     /locus_tag="JNZ73_004170"
     CDS             complement(2144..>2484)
                     /locus_tag="JNZ73_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734211.1"
                     /translation="RRWRFSIGSAPLTSITKIDAQVRGGETRQDYKDTRRFPLEAPSC
                     ALLFRPCRLPDTCPPFSLREAWRFLIAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPY
                     PVTIVLSPTR"
BASE COUNT          655 a          512 c          725 g          592 t
ORIGIN      
        1 cctggcagac gctggttaat caccattacg ggctgcatga cccgaacgcc ccggagaacc
       61 gcagggcgct gacactccct gataatctgc cggaaacgaa acaggcgctt gcagagagcg
      121 tcacacgcgg gatagacgct ctttatcatg tcggggagat aaaaggccgt caggatgtga
      181 ttcaggcgct cacagaggca gggcttgaag tggtcagggt gacgcgaagc agtatcagca
      241 ttgcagaccc gaacggtggg aagaatatca ggctgaaagg agcattctat gagcaatctt
      301 ttacagacgg gcgcggagtt cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa
      361 atgctgaacg acgagttcag gaagctcgaa gaatctgtaa gcaaggctgt gacatcaaac
      421 gagacgaaaa tcaaagacgc tatagccctg ttcacagctt cgacagagga atcactgaaa
      481 aaacaccggg aaggggtgaa agaggcgatg atgcagcaca ggaaggacgt gttaaagctg
      541 gcagggaata cgggcatgat gttactgggg atggtcattt tcctgtttac cgtgagtggc
      601 gggacgctct ggtatcttgg cgggatgata caggcgaacc tggaagaaat caggaagcag
      661 aacgagagtc tggagaagct gaacgcgaag acatggggcg tggagtttgt gcaggacggg
      721 aacaggaaat ttctggtcct tccttacggg aagtcagcag aggtgatttc ctttcagggg
      781 aaggaatggg tacatctgaa ggagtgacac agagtgacag agctggaaac acatttgctg
      841 aacgccttag agcagctgca acaggactat atgcagcggc tgagcgaatg ggagagcgcc
      901 ttcgtggaat tgcagaaaat gttttcgctt acgcaacggg acaacgcgat gctgaacgag
      961 cgggtcatgc agttgagtca gcaggtgcag cacttgagcg agcagacaga acgcttgagc
     1021 cagttataca gcgagaactg gagataagag aggaacggct gatacaggag cgcgaacatg
     1081 cgttatccct ggaacgggag cgtcagccgg aaatacagga acgcacgctg gatggtcctt
     1141 cgctgggatg gtgaaaccat gaaaaatggc agcttcagtg gattaggagg ggttaatgtg
     1201 gcctgtacca tctggttgca taggtattca tgcggttaaa atttatcagg cgcgatcgcg
     1261 gcagtttttc gggtggtttg ttgccatttt tatctgtctg ctgccgtgat cgcaccaaac
     1321 gcgtttcagt ggtgcgggca ataagatatt acgttaaatt gtgagagaat ggtccccata
     1381 tactgacttt atcatacgga tagggttggt agtgggtcga ttgggtttcg gcattgtttc
     1441 cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat tctcctgagc
     1501 ctggaaagtc taagtgttta gaattgatgg caaaatatct tccgtccgat tcttgaagta
     1561 gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt tgtagttgtt
     1621 gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt attattctct
     1681 cttttaataa gctgtttacc acaggattta gttgttgtct aaagcacgca gataatgcca
     1741 ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt gtttgatgtg
     1801 atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg gctataaaac
     1861 aaaattttca tagagtgatt caagatcttc ttgagatctt ttttggcggt gcgtaatctg
     1921 ctgcctgcaa acaaaaaaac caccgttatc aacggtggtt tgtttgccgg aatctagagc
     1981 taccaactct ttcttccgaa ggtaactggc ttggtggagc acagatacca aatgctgtcc
     2041 ttctagtgta gccgcagtta ggccaccact tcaagaactc ttaatatctc aatccacctt
     2101 atccagttac caatggatgc tgccagtggc gttaagtcgt gtcttaccgg gttggactca
     2161 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2221 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc aacagcgtga gctatgagaa
     2281 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2341 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2401 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2461 ctatggaaaa acgccagcgg cgca
//
LOCUS       DADMGC010000252         2482 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.255, whole genome shotgun sequence.
ACCESSION   DADMGC010000252 DADMGC010000000
VERSION     DADMGC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.255"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            30..1082
                     /locus_tag="JNZ73_004171"
     CDS             30..1082
                     /locus_tag="JNZ73_004171"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HAY8734212.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1158..2482
                     /locus_tag="JNZ73_004172"
                     /pseudo
     CDS             1158..2482
                     /locus_tag="JNZ73_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
BASE COUNT          586 a          558 c          643 g          695 t
ORIGIN      
        1 agttatgttt ttaaattgag ggcattatta tgaaaaaaat accctgcgtg atgatgcgag
       61 gtggaacctc gaggggcgcg ttcctgttag cggaacattt acccgaagat caaacgcagc
      121 gcgataaaat attgatggca attatgggtt ccggtaacga tctggaaatt gacggtattg
      181 gcggcggtaa tccactgacc agtaaagtcg ccattattag ccgttccagc gatccgcgtg
      241 ctgatgtcga ttatctgttt gctcaagtta tcgtccatga gcaacgtgtc gataccacgc
      301 ctaactgcgg caatatgctg tctggcgtag gggcattcgc cattgaaaat ggtttgattg
      361 cagcgacttc gccagttacc cgtgtacgta tccgcaacgt caatacgggt acgttcatcg
      421 aagctgatgt gcaaacgcca aatggtgttg tcgagtacga gggtagcgcc agaattgacg
      481 gcgtaccggg tactgccgca ccggttgcgc tcactttcct gaatgccgct ggaacgaaaa
      541 ccggaaaagt tttcccgact gataatcaga ttgattattt tgacgatgtc ccggtgacct
      601 gtatcgatat ggcgatgcca gtcgtcatta ttccggctga atatctggga aaaacaggtt
      661 atgaattacc ggcggaactg gatgccgaca aagcattatt agcccgcatt gaatctatcc
      721 ggctacaagc gggtaaagca atgggcttag gtgatgtcag taatatggtt atccctaaac
      781 ctgtgcttat ttctccagcg cagaaaggcg gggcaattaa tgtgcgttat tttatgccgc
      841 attcttgcca tcgcgcgctg gcgataaccg gtgctattgc tatttccagt agttgtgcat
      901 tggaaggcac cgtcacccga caaatcgtcc cttctgtagg atatggcaat atcaatattg
      961 aacaccccag tggtgcgctc gacgttcatt taagtaatga aggtcaggat gccacgacgt
     1021 tacgcgcatc tgttattcgg acaaccagaa aaatattttc cggtgaagtt tatcttccct
     1081 gaaaaaattc gttgtcagga taaggacaat caataaagga cttctgtatg agtcatacag
     1141 aaagaacagg attttaaatg aataagaaat cgttatggaa gctaattctg atattagcga
     1201 tcccatgtat tattggtttt atgccagctc cggcaggatt aagcgaactg gcgtgggtgc
     1261 tttttggtat ttacctggcg gccattgtgg gtctggttat caagcctttc ccggaacctg
     1321 tcgtactgtt aattgccgtt gctgcctcaa tggtggtggt cggtaactta tccgacggtg
     1381 cgtttaagac caccgccgta ttaagcggtt actcttcagg tactacctgg ctggtgttct
     1441 cggcgtttac cttaagcgcc gcatttgtga ccaccggttt aggtaaacgt attgcctatc
     1501 tgctgattgg taaaatcggt aacaccatgc tgggtctggg ttacgttacg gtattcctcg
     1561 atctggtact ggctccggca acaccgtcta acaccgcgcg tgcgggcggc attgtgttac
     1621 cgatcatcaa cagcgtggcg gtggctttgg ggtccgaacc ggaaaaaagt ccgcgtcgtg
     1681 tcggacatta cctgatgatg tccatttaca tggtcaccaa aaccaccagc tatatgttct
     1741 ttaccgcaat ggcggggaac attctggcgc tgaaaatgat caacgacatt ctgcacctgc
     1801 aaattagctg gggtggatgg gcgctggccg ccggattgcc ggattgccgg attgccggat
     1861 tgccggattg ccggattgcc ggattgccgg attgccgggc atcattatgc tgctggtcac
     1921 cccgctggta atttacacca tgtatccacc agaaattaaa aaggtggata acaaaaccat
     1981 ctctaaagcg ggccttgccg aactgggacc gatgaaaatc cgcgaaaaaa tgctgctcgg
     2041 tgtctttgtg ctggcgctac tgggctggat tttcagtaag tctctggggg ttgatgaatc
     2101 cactgtggca atcgttgtta tggcgaccat gctgctgctg ggtatcgtta cctgggaaga
     2161 cgtggttaaa aataaaggcg gctggaatac cttaatctgg tacggcggta ttatcggctt
     2221 aagctcctta ttatcgaaag ttaaattttt cgaatggtta gctgaagtct ttaaaaataa
     2281 cctggcattt gatggtcacg gtaacgttgc tttcttcgtt attattttcc tcagcatcat
     2341 cgtgcgttat ttcttcgctt ccggtagtgc ctatatcgtt gctatgttac ctgtatttgc
     2401 catgctggcg aacgtctccg gcgcaccgtt aatgttaacc gcgctggcac tgttgttctc
     2461 caactcctat ggcggcatgg tt
//
LOCUS       DADMGC010000253         2439 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.256, whole genome shotgun sequence.
ACCESSION   DADMGC010000253 DADMGC010000000
VERSION     DADMGC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.256"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..564
                     /locus_tag="JNZ73_004173"
     CDS             <1..564
                     /locus_tag="JNZ73_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY8734213.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            522..1199
                     /gene="bglJ"
                     /locus_tag="JNZ73_004174"
     CDS             522..1199
                     /gene="bglJ"
                     /locus_tag="JNZ73_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HAY8734214.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQEFFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1237..2025)
                     /gene="fhuF"
                     /locus_tag="JNZ73_004175"
     CDS             complement(1237..2025)
                     /gene="fhuF"
                     /locus_tag="JNZ73_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HAY8734215.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2166..2402
                     /locus_tag="JNZ73_004176"
     CDS             2166..2402
                     /locus_tag="JNZ73_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HAY8734216.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          627 a          532 c          627 g          653 t
ORIGIN      
        1 gtcattgctg atttagccgg tgaaagtgaa gatccacgtt ctgtttgtga acattattat
       61 tctttaatct cacaatatcg ggaaattcac tgggttttca tggtgtcacg ctcctggtat
      121 tcccaggctg tagaactgct catgtgccct acggcgacgt tattgtctga tgttgaaccc
      181 attgagaatc tggtcaagac cgtacgttcc ggcaatacgc atgcagagcg tatcagcgcc
      241 atgctgacct ctccggcaat gactgaaact catgatttta gctatcgctc cgtcattctc
      301 actctttcag agcgcaaggt actgcggcta ttaggtaaag gatggggcat caaccagata
      361 gcttcattgc ttaagaaaag taataaaact atcagcgccc aaaaaaacag tgcgatgcgt
      421 cgactggcaa ttcacagcaa cgctgaaatg tatgcatgga taaatagcgc gcagggtgca
      481 agagaactta acttgccttc tgtttatgga gatgccgcag aatggaacac agccgaatta
      541 agaagagaaa tgtcgcactc atagaaaaat gcgtcatgag tagtatcggt attgagagtt
      601 tattcagaaa gtttgcgggc aacccttata agctccatac ctataccagt caggagtcat
      661 ttcaggatgc catgtcgcgg atctcgtttg cggcggtcat tttttctttt tctgccatga
      721 gaagtgagcg gagagaggga ttatcttgcc tgactgagct ggcgattaag tttccgcgta
      781 cccggcgttt agttattgtg gatgatgata ttgaagctcg gctgattggt tcattgtcgc
      841 catcaccgct ggacggtgta ttaagtaaag cgtcaacgct ggagattttt catcaggaat
      901 tctttttgtc attaaatggt gtacgtcagg cgaccgaccg actgaacaat cagtggtaca
      961 ttaaccaaag ccggacgtta agcccgacgg agagagaaat attgcgcttt atgtcgcgtg
     1021 gctactcaat gacacaaatt gccgagcagc ttaaacgtaa tatcaaaacg atccgagcgc
     1081 ataaatttaa tgtgatgtcg aaactgggcg tcagttctga tgcagggttg ttggaggccg
     1141 cagatattct gttatgtatg cggcattgcg aagcaagtaa tgtgttgcat ccctattaat
     1201 ccgcatgatg ccgggtttac ttccccggca gtgctttcat ttcagcgtac aatagccaca
     1261 ttgctgcaca tccggtaagc gataacgctg gcagcaagtg cggcgcacca gcaggccgtc
     1321 gcgcagtacc acggtacgcc agagtggatt atcttcaccg ttcgtgagcg ttttctcaaa
     1381 aaagagggca tggcgcagcg attcaacagt agcctcgccg agcagttgct tcatctcagt
     1441 gagataccag ttgatcaaat aaccggtatt actccagata agtttgccgt tgatctctcc
     1501 ggtcgcttct agtgcttgca caaccggaac cagcgcctgg ctgattaacg tttccattcg
     1561 ctgctgcggc gaatgtggtg ttgcgttttt atcttcacac acatcgaccc agaaacaggc
     1621 gacgcgtccg gtttcgtgaa actcagcatg gaaatgttcc ggcgacacat ctaatgcttt
     1681 ttcctgcgtc agtagcgcca gcattaatgg tggcaccatc aggccgatat accattgtgc
     1741 ccatagtgag atcagcggtt tgttctcgcg gatcatcgtt ggttggttgc gatagatatg
     1801 atcggaatag accgccagta gagaacttag cgcattcggt gatgaccatt gcgccagcgt
     1861 catggcgtta agcggagcgg gttcatccag gcggataaac tccagcaaat gttcacgatg
     1921 tttggcgatc atcgcccgta cggcttgtgc aagcccagca tcctgcggct ggagatgcgt
     1981 tcgccagatg acatcttcat agagcggtgc ggaacgatag gccataatcg tgatagtaat
     2041 ctaaatgata atgattgcta atcctatcga taggtttact cgatagcaag ggatttctct
     2101 ggcttgcaaa tgataaaaat tatcatatga tattggtttt cattatcaat gaaagagatg
     2161 aaaccatgtt gcaacgtacg ctgggcagtg gctggggagt gttgctgccg ggattgctga
     2221 ttgcagggct gatgtatgcg gatttatcgc cggatcagtg gcggattgtc attctgatgg
     2281 gattagtatt gacgccggtg atgctgtatc acaaacagtt gcggcattac attttgctac
     2341 catcgtgcct ggcgcttatt gctggcatca tgctgatgat aatgaatttg aatcagggat
     2401 gacaaaacag ggaagaaaca agaaaggaag taaagataa
//
LOCUS       DADMGC010000254         2419 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.257, whole genome shotgun sequence.
ACCESSION   DADMGC010000254 DADMGC010000000
VERSION     DADMGC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.257"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..342
                     /gene="capU"
                     /locus_tag="JNZ73_004177"
     CDS             <1..342
                     /gene="capU"
                     /locus_tag="JNZ73_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY8734217.1"
                     /translation="DNVFPAAPVYRVASLVVLPSENESFGMVLAEASAFSVPVVATQI
                     GGIPEVIQNNQTGTLLPASNKHAWMCALNDFFNDPGRFYQMARLAKQDIEERFDINKT
                     ALKILTLAKQK"
     gene            347..1297
                     /gene="virK"
                     /locus_tag="JNZ73_004178"
     CDS             347..1297
                     /gene="virK"
                     /locus_tag="JNZ73_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY8734218.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            1362..2306
                     /gene="lpxM"
                     /locus_tag="JNZ73_004179"
                     /gene_synonym="msbB"
     CDS             1362..2306
                     /gene="lpxM"
                     /locus_tag="JNZ73_004179"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY8734219.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          704 a          469 c          545 g          701 t
ORIGIN      
        1 gacaatgttt ttcctgccgc ccccgtatat cgggttgcca gtctggtggt tctgccttca
       61 gaaaacgaat cttttggtat ggtgctggca gaagcatcgg cattttctgt gcctgtagtg
      121 gccactcaga ttggtggaat ccctgaggtt attcagaaca accagaccgg gacattgtta
      181 ccagcaagta ataagcacgc atggatgtgc gccctgaatg atttttttaa tgaccctggg
      241 cgtttttatc agatggctcg cctggcaaaa caggatatag aagagcggtt tgatattaat
      301 aaaactgcgt taaaaatact cacattagcg aagcaaaagt aacaatatgt tttctataag
      361 taacttatca tttatcggtt tccttaaaag gattattttt tcctcagatt cactaccggg
      421 gaagtgggaa cacagaaaat ttcggttcat gtacattttg cgatgttcta taaatccggt
      481 tgtcagtatt cgatattatt atgaactgcg ttccttgccg tgcattgagg atattctggc
      541 aatacacccc acgttgccag caagaattca tcgcccttat ttgcataaag gggggcgcgc
      601 ctggacccgt ggacaatata ttcttgagca ttatcgcttt gtgcaaaatt tgccagaaaa
      661 atattctaaa tttttgtttc cacaaaaatc ggtatcatta gtccaattca tcgggaaaga
      721 tggagagaat tttgatatcc agtgttctcc cagtggtttt gaccgggaag gagagttgat
      781 gttgtcattg ttttacaata aaacagttat tgcccggtta acattttcag tcattctgac
      841 tcaaaatgga cacattgctt ttattggcgg attacaggga gcccctaaaa ataccggacc
      901 tgatgttatt cggtgcgcaa cccgggcctg ttatgggatt ttcccaaaac ggattatttt
      961 tgaagcattt tgtgcgttga tgaaagcttg caatatctct gagtgtctgg cagtcagtga
     1021 gcacagtcat gttttccggc aattgagata ctggtatcag aaacgcaaaa cctttgttgc
     1081 ggtctatagt gatttctggg agtctgtagc aggaaaaacc tgtggtgact ggtacagatt
     1141 accaacccag gtgattcgaa agccgctaag cgatatagcc agtaaaaaac gctctgagta
     1201 ccgaaaacga tacgctttgc tggattatat ccatgaaact actatccgct ctttggatgc
     1261 atatcctgta cactcagaac atcagaattt aaattaaggt ttgatgtatt ctctgaataa
     1321 aacagtaatg ataattttgg tagaggcatt cgcactaaat aatgaaaaaa tataaatctg
     1381 agtttattcc tgaatttaag aaaaattacc tttcccctgt ttactggtct acatggttcc
     1441 ttttgggaat gattgcaggt atttcaatgt ttcccccttc attcagagat cctgtcttgg
     1501 caaaaatagg gcgttgggcg ggcagattga gcaaaaaagc tcgtcgcagg gcgacgatta
     1561 atttatcgct ttgtttccca gaaaagagtg atacagaacg ggaaataatt gtcgacaaaa
     1621 tgtttgtcac agcattgcaa tccatagtga tgatggcaga actggcaatt cgtggtccgg
     1681 aaaagttcca gaaacgagtg ttctggaaag gcctcgaaat tcttgaggaa attcgacaca
     1741 ataatagaaa tgtgattttt ctggttcccc atggctggag cgtggatatt cctgcaatgt
     1801 tgctggcagc tcagggaaaa aaaatggccg ccatgtttca tcagcaacga aatccagtga
     1861 ttgattatgt ctggaattca gtacggcgta aattcggggg gcgcttacat gcccgggagg
     1921 atgggataaa accatttatt cagtcagtac gccagggata ctggggatat tacctcccag
     1981 atcaggatca tggtcctgaa tacagtgaat ttgctgattt ttttgcgacc tataaagcga
     2041 cattaccaat cattggacgt ctgatgaaca tcagtcaggc tatgattata ccacttttcc
     2101 cggtttatga tgaaaaaaaa catctactga ctattgaaat tcggccacca atggatgctt
     2161 gcattgccag cgcggacaat aaaacgattg cccgacaaat gaacaaaaca gtggaaattt
     2221 tggtggggcc acatccggaa cagtatgtct gggttttaaa attgttaaaa acacgcaaat
     2281 caaacgaagc ggacccgtac ccttgaaatt ataaagtaca agtatttcca ctagttgttt
     2341 gtaagcgtac agcctgaacc gtctggtcag aatctgacga attagacaaa gtggtgtcca
     2401 ccaaataagt agtgggaac
//
LOCUS       DADMGC010000255         2410 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.258, whole genome shotgun sequence.
ACCESSION   DADMGC010000255 DADMGC010000000
VERSION     DADMGC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.258"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(344..610)
                     /gene="ospE2"
                     /locus_tag="JNZ73_004180"
     CDS             complement(344..610)
                     /gene="ospE2"
                     /locus_tag="JNZ73_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HAY8734220.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(792..1193)
                     /locus_tag="JNZ73_004181"
                     /pseudo
     CDS             complement(792..1193)
                     /locus_tag="JNZ73_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1622..2410
                     /gene="virF"
                     /locus_tag="JNZ73_004182"
     CDS             1622..2410
                     /gene="virF"
                     /locus_tag="JNZ73_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HAY8734221.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          741 a          431 c          446 g          792 t
ORIGIN      
        1 cccctaagcc gtctatggga aggagtctgc tgttattcca gagaatggta attcagtcat
       61 atatttctcc atctatgact tgtcaatacc atcctgatat ttgtgggtca gcaatatatt
      121 ctactaaaag gtatttaagg tcattgcgtc cctgcactcg aataatggtg aagaacgcat
      181 gataaatata tcacgataac gttcagtatg tatcgaggat agatggagtt actcctctct
      241 gaaatagaat tactgctcta ctttttctgt ttccagaaat tattagtaac aaaccctgat
      301 atattatttt gaaaatggag cttaaaccag tctgaaatta acttcagaaa tcagaatacc
      361 gttggctttg tttttcatca attccggctt catttatctt tgtcaaccat tgcatttctg
      421 tagcagtgaa tttcaacgtt ttcgtccggt tggcagcagg tatttgtaat tcgtatatag
      481 ctgctttttt attgaaaaca gtatatccat ctgtagtaac agtagaagat aataatacag
      541 gatttgatac ttcaaggatg atattttctt gtttttgtgg aaggcaaggg aatatagttt
      601 gtgtaagcat attgcttcct tatgttatat tttagtaaag aatttatttt ttacattata
      661 gtcggctaaa tgaagtttac tgtaaaaaaa cgatactttt ttcgtaagcg atgagatgtg
      721 gagccagaaa aatggctgcg ttacgtcatt gaactgtcct tgacccacga tattcagaca
      781 gtcgttgtat cttaagttga cattacccgc tgacgtcgat attcccttgg tgagagataa
      841 cccaacgcac tatgcggatg gttttcattg taatgctcga tcgccactgc aaggttatgc
      901 aatgccgttc ttggtttcgg catgaacgcg atgcagtctt ccttcatcgt tttcacgaac
      961 cgttctgcta ttccattact ctgagggcta ctgactgccg tcgtgcaggg ctccagattc
     1021 aactctctgg cgaactgccg tgtttcatga gccgtataag ccgaaccgtt atcggccact
     1081 ggattggccg ttccggtcct ttatcgccat atcactcctg acatgacatc ctgcaccgtt
     1141 gctttgtcat aatctcccgt gcctgctgcc cagtctatgg cttcacgatc gcaatatgga
     1201 acgtaacgct gtcatcggga gaatcgtcaa tgcggcattc ggttaacgct taccaatatc
     1261 aacaccaacc ggatttgtgg agcctccagt ctgaaggggc tttatgcgtt ccgtatagga
     1321 tattatgatg ctggagtttt gcgaagctcc actcctttcc gaacatttac acagatattg
     1381 cctaagaaaa gtgaaaccat aatatggtta tagtcccttt cagtgcaaat acttagcttg
     1441 ttgcacagag aaatagaagc tgcataagct ctttcttcaa aaaatgtaaa taaagttaaa
     1501 tataggaaaa attacttaat ctatcttaat aacggaaaga ttttgtatac aatcactgtt
     1561 acagaaattt cttagttact ctgtaaacac taaatatagt ttggtatatt ctgttgaatt
     1621 tatgatggat atgggacata aaaacaaaat agatataaag gttcgcttgc ataactatat
     1681 tattttatat gcaaaaaggt gttcaatgac ggttagctca ggcaatgaaa ctttgactat
     1741 cgatgaaggg caaattgctt ttatagagcg aaatatacaa ataaacgtct ccataaaaaa
     1801 atctgatagc attaatccat ttgagattat aagccttgac agaaatttat tattaagcat
     1861 tattagaata atggaaccaa tttattcatt tcaacactcc tattctgagg agaaaagggg
     1921 gttaaacaaa aaaatattcc tcctctctga ggaggaggtt tctatcgatt tgttcaaatc
     1981 tataaaagag atgcctttcg gcaaaagaaa gatctatagt ttagcttgcc ttttatcagc
     2041 tgtttctgat gaggaagctt tatatacttc gatatcgata gcttcttctc ttagtttttc
     2101 tgatcagata aggaagattg ttgaaaaaaa catcgagaag agatggcgtc tttctgatat
     2161 ttcaaataac ttgaatttat cagaaatagc tgttagaaaa cgattggaga gtgaaaaatt
     2221 aacatttcaa caaatccttc ttgatattcg catgcatcat gcagcaaagc ttttattgaa
     2281 tagtcaaagc tatattaatg atgtatcaag acttatcgga atatcaagcc catcttattt
     2341 tataaggaaa tttaatgaat attatggtat aactccaaag aaattttact tatatcataa
     2401 aaaattttaa
//
LOCUS       DADMGC010000256         2373 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.259, whole genome shotgun sequence.
ACCESSION   DADMGC010000256 DADMGC010000000
VERSION     DADMGC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.259"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..823)
                     /locus_tag="JNZ73_004183"
     CDS             complement(<1..823)
                     /locus_tag="JNZ73_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY8734222.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1424..1672)
                     /locus_tag="JNZ73_004184"
     CDS             complement(1424..1672)
                     /locus_tag="JNZ73_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY8734223.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2027..>2373)
                     /locus_tag="JNZ73_004185"
     CDS             complement(2027..>2373)
                     /locus_tag="JNZ73_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY8734224.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          613 a          438 c          573 g          749 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggttg tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggcgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tgattccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggagggg gcaatttttc cttaaggtat gaaaaaagga
     1081 gctgaagcta tataatagct attaatgcca ctggtatcag tgaatcatgt atataaaaaa
     1141 ctgtgttaag gaggctgtcc attagcggtt ttttattctt ctggaaccgc ggcatctttg
     1201 cgtatctttt tgtcttctca ccgtctggtc ggtgttttgc tgagactgct aatttcctgt
     1261 ctttgctagt gctgtactgg tactgcccaa tcatgattgg cgggggcggg cggagttgag
     1321 accgcagaca cgtcgtatgc aagaacgtgc tgcggctggc tggtgaactt ccgatagtgc
     1381 gagtattgaa tgatttccag ccgttactga ttttacgtgc taattagtga acaaaccact
     1441 cgtcagcaga ctcccaggta tctttcagag tttcctgaac aaaagtttta gctgaatctt
     1501 tatcggcggt gcgcgtaaca gaaaggccat cgttgctggt ggcttttacg atcacctcta
     1561 catcgtcata acgtttactg atgcgtcggg ttaattcttc ctttaacgca tccacagcac
     1621 cggttggcat tttagtcatt ttctctttgg ctatgcagat ttcaatacgc ataaaaatcc
     1681 ctctacactg tgtttttata cagtattatt tttaactgta tggataaaca gtgtcaagag
     1741 tgagggaaag tgattttttg gggcatgcat ggggcaaaaa tatttgttgt ggggcatttt
     1801 ggggcatgat tgggacactt tgttacatat gaactttgcc gattttcata tgacatggca
     1861 atacaacgct ttgattattc taaagaaaac acatgctctt gggcgttctt tagttatttt
     1921 taaaaatccc gtatcacgca gttaaagtgg cgggcatact cttcaaggct ggtgatcccc
     1981 aaccgcaccc atttaggatg cgaccattgc ggtagaccga tgtaaatcat aacgcgtgaa
     2041 ggatctcttc cacgctacgc acacgggcga tgcgcgggta gatatgatta atgctgttat
     2101 tgtgttgctc ggcgctggcg gcactacagg cgtcttcggc aatcaccaga ttaaagccaa
     2161 gttcccaggc attgcgggcg gtggattcaa caccgatatt ggtcgagatc ccacataaca
     2221 ctattgtatc gatacctcgg cggcgcagtt gcaactccag atccgtaccg taaaacgcac
     2281 cccactgacg tttgatgatt tcgatatcac tgtcagttgc acctaatgca gctggatgtt
     2341 gccaccagtt ttcgggcaac actttagcgg ggg
//
LOCUS       DADMGC010000257         2331 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.260, whole genome shotgun sequence.
ACCESSION   DADMGC010000257 DADMGC010000000
VERSION     DADMGC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.260"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..156
                     /locus_tag="JNZ73_004186"
     CDS             <1..156
                     /locus_tag="JNZ73_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY8734225.1"
                     /translation="TSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYI
                     RIQCLGG"
     gene            complement(245..757)
                     /locus_tag="JNZ73_004187"
     CDS             complement(245..757)
                     /locus_tag="JNZ73_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8734226.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            952..1716
                     /locus_tag="JNZ73_004188"
                     /pseudo
     CDS             952..1716
                     /locus_tag="JNZ73_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1667..>2331
                     /locus_tag="JNZ73_004189"
     CDS             1667..>2331
                     /locus_tag="JNZ73_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAY8734227.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          659 a          518 c          590 g          564 t
ORIGIN      
        1 acctccagcg cctccggcat gggcaatgat gccaccgtcg aactctctcc agttgctgga
       61 cgaaacgttc tcggtatccg ggacggaatc atcagcgacc agacagcatt gagaatgctt
      121 caggagtaca tcaggatcca atgccttggg gggtagcggt aattttactc attatccttc
      181 aaatcagatt ctgttgtcag aggaatgggg gaagctggat tcgcaagagc tttttatggg
      241 cagtttagct aaaaaaaggc aacacgaagc taaaactacc aacataaagt gctgaccagc
      301 tagcagctga aatcagactc actgtataca ctttcactgg atgaagttgc aacattcctg
      361 ccaccaaagg agcaatgtaa cgcagcacgg cgataaagcg cgaagtgaaa agtattgaca
      421 cagagttatt ttgcaactga aggcgaaccc gttctattgt attggaacgg ctcgatatta
      481 tacatgcaag ttgagggata tgcccaatta ttataccaag atggtaatta acgatagtac
      541 cgcaccatgc gccactcatg accgtgattc cagcttccca tggtgaaaga gtcgtcttgc
      601 tgactgtaat tacagtggcc atcataattg atgctggtgg caatatcgcc gaaatgagta
      661 aagtggattt tgtgaacgct attataaaaa gaagccccca taggcgatat ggatgaagta
      721 caaaatagtt catcaaagca tttatccact ccatcaatgt ctttccttct gtgaattttt
      781 ctttgctatt taaccgtgat acgacttaat ttaggcttaa acaccttaca tttacaaggt
      841 actcctgtgg ggggccctgc cacggagcgt cgcaggagtg agtgatgagg aagaattaaa
      901 gcaacctatt gtgacaggca acgataacag taaatcacag gaagcataat catggcaaaa
      961 ccggactggg aggccatcga atcggcatac cgggccggag tccttagcct ccgtgatata
     1021 ggcgagaaat acggcgttac agaaggggct atcaggaaga gggccaaaaa gcttggttgg
     1081 gcacgcagtg gcggtacgca ggtttgcaaa aatggtacgc aaaaaaggaa agtgcgtacc
     1141 agcagaaagc ctgccattac tggccttaca caaaaaagta cgcaactaaa aacagaatct
     1201 acaccggata cgaaaccgat acgcggaatg cgtaccgatc ccccgactaa cccattccaa
     1261 cccggtaacc agcaggcatt aaaacacggt ggttatgccc gtcgccttct gctcaaagat
     1321 gaggtgatag aggacgctaa agcgttgacg ctcgaggacg aattatttcg ccttcgtgct
     1381 aacaaccttg tcgctgcaga gaatattggt cggtggttgg tgtcgctgga agatgctaat
     1441 ggggactagg aaaggaagat gctgattgaa aatatcagcg ccgccgagaa agcaatgatg
     1501 cgcaatacag ttcgtattga gtccatcgtt ggcacgcttg cgacggtagg aaaaatattt
     1561 gctgatacag cctaccgcaa ggccgccact gataaggtgt ctctggaggc tgatcgtctt
     1621 cgccgtgatg caggtattga tgatggcaat ggagagcgtg acctcaatga cttctactct
     1681 gacatccaaa ccgacgctga atccggcttt acgtagtttc tggactatgc gggcacgtaa
     1741 caaagtgctt tatggtggtc ggtcatcgtc aaaatcatgg gatgccgctg gcattgccat
     1801 atttctgtcg aataaataca ccctgcgttt ttgttgtgcc cgtcagatcc agaataaaat
     1861 cgaagagtcg gtgtataccc tgctcaaaat tcagatagac aggtttggtc tgcggcaccg
     1921 tttccgtatt ctgaacaaca aaatcattaa ccgggttact ggctcggaat ttgtttttta
     1981 tggattatgg cgcaacatcg aagaaattaa gtcactggag gggatcgatg tgttgtggct
     2041 ggaagaagcc cacgcactga cggaatacca gtggaaaatt ctggagccaa cgatccgtaa
     2101 agagggttcg gaatgctggt tcatattcaa ccccggactt gttactgatt tcgtctggcg
     2161 caacttcgtt gttgatccgc ccgaaggcac tctcatccgc aaaattaact atgacgaaaa
     2221 tccgtttctg tctgacacca tgcttaaggt tatcgacgcg gcgcgacgcc gtgatccgga
     2281 tggttttaaa catgtgtatg agggcgttcc ggagtctgat gatgatgcgg c
//
LOCUS       DADMGC010000258         2326 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.261, whole genome shotgun sequence.
ACCESSION   DADMGC010000258 DADMGC010000000
VERSION     DADMGC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.261"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="JNZ73_004190"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="JNZ73_004190"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY8734228.1"
                     /translation="MQRKLATWAVTDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          583 a          573 c          686 g          484 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacgctt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagtc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgctatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac gccctgcctc ttactctact cagcacatgg
     2281 cggggcttcg tctatgtggc gttcatcatt gatgtgtttg ccggat
//
LOCUS       DADMGC010000259         2300 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.262, whole genome shotgun sequence.
ACCESSION   DADMGC010000259 DADMGC010000000
VERSION     DADMGC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.262"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..785)
                     /locus_tag="JNZ73_004191"
     CDS             complement(<1..785)
                     /locus_tag="JNZ73_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY8734229.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMS"
     gene            1133..2005
                     /gene="rluF"
                     /locus_tag="JNZ73_004192"
     CDS             1133..2005
                     /gene="rluF"
                     /locus_tag="JNZ73_004192"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HAY8734230.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(2138..>2300)
                     /locus_tag="JNZ73_004193"
     CDS             complement(2138..>2300)
                     /locus_tag="JNZ73_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HAY8734231.1"
                     /translation="ETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWS
                     ANTSTRGRR"
BASE COUNT          562 a          588 c          576 g          574 t
ORIGIN      
        1 tcgctcatat tcgtttcaac cattgcgccg tgaatatcaa aaaagcgcga gcaaatatcg
       61 ccagcaacct ggcgggcatt caggtcgtca ctctcttcac caccataaaa cgcatgccag
      121 ttagcgccgt cgcgaatggc cggtgagcca attcccacca gggcgacatc cagattatcc
      181 cagtaggcag agatggtttt aaagtgctga gactgcatga tcccattacg aattaatggg
      241 ttatccagca gagccggaaa atccgcgaga tgcgattccc ctttcagctt cgctgccgcg
      301 ctgtaggtta atgtgtttac atgatagcgg ctttcgagtt taccggacgg tccgccaata
      361 attggtacgc agattaactg ccgcgattgc cccgcctgcg gcaagttttc aaccagtgcg
      421 ctcaccgcgc gaccccagga aaaaccgaca atatcgccag gctccagcaa gcgatccagc
      481 agttgcgcgc cgtgtaaccc catcatcgcc agttgagtct cttcatcctc atcatttccc
      541 gacaccacca caacgtcttt caggccaaac ttttgcttca gttgctgctc cagccagaga
      601 ttttcgttgt agtcatagtt gatggcgatg gtgaccattc cctgatcgcg gcctcgttta
      661 agcaagcggc tgatggtggt gcggtaaata cccagttcgc gcgcgatttg cgcctgcgtc
      721 atatcctgtt cgtaatagag ttgggcaatc ttcacaatca aacggatatc gtcactgttt
      781 tccattgccg cctcctggtt ctgcacattt gtgcaaatac gctttctcac cgcttgatct
      841 gatataaccc catcatgagg gcctttcaaa gcgatttttc actctttgca tggggagaaa
      901 tagcgctttc tcttgcacat ttgtttctct gcgtagtagt catcagcaca ggtgtgcagc
      961 gattcgtaat atgttgatta tccttatttt gccaggcaaa tttgcatcgt acttccggag
     1021 acacttttgt gataaaaggc gtggcagatc acatttaatt tcgacgttgc cgcataaccc
     1081 tgtttttccc cgctaagagg tacggtatat tccgcgtttt acaggagaat ttatgctgcc
     1141 cgactcatca gtccgtttaa ataaatacat cagcgaaagc ggaatttgct cacgccgcga
     1201 agcggatcgc tatatcgagc aaggcaatgt gttccttaat ggcaagcgag ccaccattgg
     1261 cgatcaggtg aaacccggcg acattgtgaa agtaaacggt cagttgattg agcctcggga
     1321 agccgaagat ttggtactta tcgccctgaa caagcccgtc ggtattgtca gcaccaccga
     1381 agatggcgag cgcgataaca ttgtcgattt cgttaaccac agcaaacgcg tgttcccgat
     1441 tggccgcctg gataaagact cccaggggct gattttcctc accaatcacg gcgatctggt
     1501 gaataagatc ctccgtgcgg gtaacgatca tgagaaagag tatctggtga cggtcgataa
     1561 accgattacc gatgaattta ttcgtggtat gggggcgggg gtgccgatcc tcgggacggt
     1621 gacgaaaaag tgcaaagtca aaaaagaagc gccgtttgtc ttccgcatta ccctagtgca
     1681 ggggctgaac cgtcagatcc ggcgcatgtg cgagcatttc ggctatgaag tgaaaaagct
     1741 ggaacgtacg cgcattatga acgtcagctt aagcggcatt ccgctggggg agtggcgcga
     1801 tttaaccgac gatgagttaa tcgacctctt taagctcatc gaaaattcct cttccgaggc
     1861 aaaacctaaa gcgaaggcca aaccgaaaac agcgggcatc aaacgtccag tcgttaagat
     1921 ggaaaaaacg gcggaaaaag gcggtcgccc ggcgtccaac ggtaagcgtt ttacctcgcc
     1981 ggggcgtaaa aagaagggac gctgattaac gcctcagact gatgaaaacg tccaaaaaga
     2041 gctcggacag tcccctcgcc ccttcgggga gagggttagg gtgaggggaa gattgtgtcc
     2101 acacagacga gtgaaaatct caggcgttga ttgttcatta gcgcctgccg cgcgtcgacg
     2161 tatttgctga ccagctaaat gacgcttcag gatccggttt ataagcctgc tttttcttca
     2221 attggcgggc ttttttcgct tcagcctcac gctcgaccat taatttatcg atgtactctt
     2281 tcttaatgct gttggtttcg
//
LOCUS       DADMGC010000260         2251 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.263, whole genome shotgun sequence.
ACCESSION   DADMGC010000260 DADMGC010000000
VERSION     DADMGC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.263"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            133..207
                     /gene="tnaC"
                     /locus_tag="JNZ73_004194"
     CDS             133..207
                     /gene="tnaC"
                     /locus_tag="JNZ73_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HAY8734232.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            428..1843
                     /gene="tnaA"
                     /locus_tag="JNZ73_004195"
     CDS             428..1843
                     /gene="tnaA"
                     /locus_tag="JNZ73_004195"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HAY8734233.1"
                     /translation="MENFKHFPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1934..>2251
                     /locus_tag="JNZ73_004196"
     CDS             1934..>2251
                     /locus_tag="JNZ73_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAY8734234.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWSAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYIS"
BASE COUNT          623 a          498 c          519 g          611 t
ORIGIN      
        1 taatctttaa aaaaagcatt taatactgct ccccgaacga ttgtgattcg attcacattt
       61 aaacaatttc agaatagaca aaaattctga gtgtaataat gtagcggcgt gtctttcgag
      121 gataagtgca ttatgaatat cttacatgta tgtgtgacct caaaatggtt caatatcgac
      181 aataagattg tcgatcaccg cccttaattt acccttctgt agccatcacc agagccaaac
      241 cgattagatt caatgtgatc gattagtttg ctatatctta attttgcctt ttgcaaaggc
      301 catctctcgt ttatttactt attttagtaa atgatggtgc ttgtatatat acatggtgaa
      361 ttaatggata ttgcagatgt aatattcaca gggatcactg taattaaaat aaatgaagga
      421 ttatgtaatg gaaaacttta aacatttccc tgaaccgttc cgcattcgtg ttattgagcc
      481 agtaaaacgt actactcgcg cttatcgtga agaagcaatt attaaatccg gtatgaaccc
      541 gttcctgctg gatagcgaag atgtgtttat cgatttactg accgacagcg gcaccggggc
      601 ggtaacccaa agtatgcagg cagcgatgat gcgcggcgac gaagcctaca gcggcagccg
      661 cagctactat gcgttagccg agtcagtgaa aaatatcttt ggttatcaat atactattcc
      721 gactcaccag ggccgtggcg cagagcaaat ctatattccg gtactgatta aaaaacgcga
      781 gcaggaaaaa ggcctggatc gcagcaaaat ggtggcattc tctaactatt tctttgatac
      841 cacgcagggc catagccaga ttaacggctg taccgtgcgt aacgtctata tcaaagaagc
      901 cttcgatacg ggcgtgcgtt acgactttaa aggcaacttt gacctcgaag gattagaacg
      961 cggtattgaa gaagttggcc cgaataacgt gccgtatatc gttgcaacca tcaccagtaa
     1021 ctctgcaggt ggtcagccgg tttcactggc aaacttaaaa gcgatgtaca gcatcgcgaa
     1081 gaaatacgat attccggtgg taatggactc cgcacgcttt gctgaaaacg cctatttcat
     1141 caagcagcgt gaagcagaat acaaagactg gaccatcgag cagatcaccc gcgaaaccta
     1201 caaatatgcc gatatgctgg cgatgtccgc caagaaagat gcgatggtgc cgatgggcgg
     1261 cttgctgtgc atgaaagacg acagcttctt tgatgtgtac accgagtgca gaaccctttg
     1321 cgtggtgcag gaaggcttcc cgacatatgg cggcctggaa ggcggcgcga tggagcgtct
     1381 ggcggtaggt ctgtatgacg gcatgaatct cgactggctg gcttatcgta tcgcgcaggt
     1441 acagtatctg gtcgatggtc tggaagagat tggcgttgtc tgccagcagg cgggcggtca
     1501 cgcggcattc gttgatgccg gtaaactgct gccgcatatc ccggcagacc agttcccggc
     1561 acaggcgctg gcgtgcgagc tgtataaagt cgccggtatc cgtgcggtag aaattggctc
     1621 tttcctgtta ggccgcgatc cgaaaaccgg taaacaactg ccatgcccgg ctgaactgct
     1681 gcgtttaacc attccgcgcg caacatatac tcaaacacat atggacttca ttattgaagc
     1741 ctttaaacat gtaaaagaga acgcggcgaa tattaaagga ttaaccttta cctacgaacc
     1801 aaaagtattg cgtcacttca ccgcaaaact gaaagaagtt taattaatac tacagagtgg
     1861 ctataaggat gttagccact ctcttaccct acatcctcaa taacaaaaat agccttcctc
     1921 taaaggtggc atcatgactg atcaagctga aaaaaagcac tctgcatttt ggggtgttat
     1981 ggttatagca ggtacagtaa ttggtggagg tatgtttgct ttacctgttg atcttgccgg
     2041 tgcctggttt ttctggagtg cctttatcct tatcattgcc tggttttcaa tgcttcattc
     2101 cgggttattg ttattagaag caaatttaaa ttatcctgtc ggctccagtt ttaacaccat
     2161 caccaaagat ttaataggta acacctggaa cattatcagc ggtattaccg ttgccttcgt
     2221 tctctatatc ctcacttatg cctatatctc t
//
LOCUS       DADMGC010000261         2246 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.264, whole genome shotgun sequence.
ACCESSION   DADMGC010000261 DADMGC010000000
VERSION     DADMGC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.264"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..444
                     /locus_tag="JNZ73_004197"
     CDS             <1..444
                     /locus_tag="JNZ73_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY8734235.1"
                     /translation="PGEQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPES
                     PAQYLQRQKPQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVI
                     PLCRKCHNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            445..810
                     /locus_tag="JNZ73_004198"
     CDS             445..810
                     /locus_tag="JNZ73_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY8734236.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            807..1523
                     /locus_tag="JNZ73_004199"
                     /pseudo
     CDS             807..1523
                     /locus_tag="JNZ73_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
     gene            1717..1792
                     /locus_tag="JNZ73_004200"
     tRNA            1717..1792
                     /locus_tag="JNZ73_004200"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1750..1752,aa:Ile,seq:cat)
     gene            1794..1870
                     /locus_tag="JNZ73_004201"
     tRNA            1794..1870
                     /locus_tag="JNZ73_004201"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1828..1830,aa:Lys,seq:ttt)
     gene            1873..1948
                     /locus_tag="JNZ73_004202"
     tRNA            1873..1948
                     /locus_tag="JNZ73_004202"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1906..1908,aa:Thr,seq:tgt)
     gene            1952..2026
                     /locus_tag="JNZ73_004203"
     tRNA            1952..2026
                     /locus_tag="JNZ73_004203"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1985..1987,aa:Gly,seq:tcc)
BASE COUNT          604 a          486 c          604 g          552 t
ORIGIN      
        1 ccgggagagc agaccgccac cagcatactg aagcagcgca caaaaaatct tgcgccgctg
       61 cctcacgccc accagcaaca gaatccacca caggaagaga cggtggtcag cattgccgtt
      121 gatccggaat ctccggctca gtatctccag cgccagaaac cacaacggga agagatgcct
      181 gtatacacgc gctgggtaaa aacgcagaaa tgcatgacgt gtggcaatca ggcagatgat
      241 ccgcaccaca tcattggtca tggactggga gggatgggaa caaaggctga cgatttgttt
      301 gttattccgc tgtgtcgtaa atgccataac gaactacacg ccggggtaaa agattttgaa
      361 gaaaaacacg gtagtcagtt gttgttgctg attcgttttt taatgcacgc gagaaattcg
      421 ggtgttctga agtggaaagc ataaatgacc gaacgcatag aatttgtttt gccttacccg
      481 ccaacggtga acacctactg gcgacgtcgt ggcagcacat attttgtatc aaaagccggt
      541 gagcgttatc gccgtgatgt ggcgctaatt gttcgccagc agcgactgaa attaaacctg
      601 tccggaaggc tggcgataaa gattattgca gagccaccgg ataagcgccg tcgtgacctg
      661 gacaatatcc tgaaggcacc gctggatgcg ctgacgcatg ctggactact tatagacgac
      721 gagcagtttg atgaaatcaa tattgtgcgc ggtcagctcg ttcctggtgg gcggctgggg
      781 ataaaaatca cagaactggg gtgcgcatga ataaccagta tttacagttt gtgcgtgagc
      841 agctcattat cgccaccgct gatttgagtg gggcaacaaa aggtcagctt gaagcctggc
      901 aagagaatgc catgttcgat acagggcgtt acaggcgtaa aaaaatccgg taccgcgatg
      961 aagtgactgg aaaaatgata acgcgggata atccaccaat cccgggaaag caatcgctgg
     1021 cgaaggggac gtcaattcct ctggtcagtc cggttgagtt ttcgacatca tcgtggcggc
     1081 gggctgttct gtctcttgaa gaacatcata aagcctggtt gttgtggtgt tacagcggga
     1141 gtatttgttg ggaatatcag atcgcgataa cacagtgggc gtggaatgaa tttaatgctc
     1201 aatccggtac cagaaaaatt gcagggaaaa cgcaggaacg cctgaaaaaa ttaatctggc
     1261 tggcggcgca ggcagtaaaa gcagaacttt ttggtgggga aggttatgaa taccaggttg
     1321 gtggggaagg ttatgaatac caggagctgg catttactgg cgggagtgac aactaaaaac
     1381 tggtccaaaa catttactcg tcactggatt gcaatgaaac acatttttca ccgactggat
     1441 agtgaggctt tattgtttgt aatgagaaca cgttcaaaaa aaagacggca ttttcaaagc
     1501 aaagtgttgc aaaagtagat tgaaaggcat atattcatgc aaatctgata ttttgccgat
     1561 tttttacgtg atggcaaaag caaacaaaac ccgcccacaa gcgggttttt tgtgccactt
     1621 atctcggata gacatggtga atgcgctggt ggaggagata agggtgattt ttgaatgctt
     1681 gcaacattga tttcgtaacg ttattatcct gcgcccggcc ctttagctca gtggtgagag
     1741 cgagcgactc ataatcgcca ggtcgctggt tcaaatccag caagggccac caaccgccac
     1801 tagctcatca ggaaagagcg tcaacccttt aagttgagtg tgcgaggttc gagtccccgg
     1861 tggcggtcca gtgccgactt ggctcagtag gtagagcaac tgacttgtaa tcagtaggtc
     1921 accagttcga ttccggtagt cggcaccata tgcgggcatc gtataatggc tattacctca
     1981 gccttccaag ctgatgatgc gggttcgatt cccgctgccc gctccagtta gagtctttca
     2041 gtctgcgatg atgggaaatc ccggagtgac tgaaagacgt ttaagttatg aatgatcgct
     2101 tttttttgca aaattgctgt gcagaaatac taaccttcgg gcaggcgatc attcataagc
     2161 actctgcttt tattccgatt aactgtgagt ggtttgttgg atagagtgct ttccttactg
     2221 tatatatcgt ttcgcccgct tttgcg
//
LOCUS       DADMGC010000262         2186 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.265, whole genome shotgun sequence.
ACCESSION   DADMGC010000262 DADMGC010000000
VERSION     DADMGC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.265"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(219..383)
                     /locus_tag="JNZ73_004204"
     CDS             complement(219..383)
                     /locus_tag="JNZ73_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734237.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1285..1569
                     /locus_tag="JNZ73_004205"
     CDS             1285..1569
                     /locus_tag="JNZ73_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY8734238.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1557..2042
                     /locus_tag="JNZ73_004206"
     CDS             1557..2042
                     /locus_tag="JNZ73_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8734239.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSINHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          564 a          461 c          621 g          540 t
ORIGIN      
        1 caaaatgatg gacattattt ttgtggaacc ggaggtaaca gaccagacgg tttaaatgag
       61 ccggttactt taaacgggtg atggacagca cctcaaaagt tcagatggat tatatttgtc
      121 tgcaataccc cgggtatttt cgatttgcga aatggatgga acgacttgct caggggatag
      181 ctgatggagt tactgaagta ccgaaggacc actaaaaatc acaggccggg aatttcggcg
      241 tttccggcgt aggtgtgcca cgaatgcatc cagtggcgtg atgttggatg caatgctgta
      301 tgagagatga ggggcggcgt ccagacgcag ccttcaaacc gccttgagct gctgccgtta
      361 agagcggtgg tggtgagcgt caaggttaaa aggcgcagaa catgtgtcag gtaggtgttg
      421 cggagatgaa cgaaagtgaa ctgtcgatga agtgtcgaaa ggaacctcta ggcgatgtcg
      481 aaaatgtgct atctacgcag cgcatgacga gtccagcaga tatctggtac tggctgggcg
      541 gcatccggca tataggcagc atgaacgcaa cacaggctct cgtacggaac gtgggaacct
      601 gtcgtctcga tgctaaggga gcggcgcgga gcagaggcac tgcaagcgcc tgagtaccga
      661 tgcgagacac aggggcagat ggcttcgtag tagtgaggaa gtgcctgtaa tgggcatgga
      721 gcgaaggggg tcagtcgttg gggctggttt atcggtcaac cggatagccg ggaggagccc
      781 atgagtcagc caaagccgtt cgaggtctct aagtatgcgg tttggaaagc ataccagcgg
      841 gtcaaagcta atcgaggtgc ggcaggtgtc gatgggcagt cagtcgaggc gtttgaggtt
      901 accaatcgaa gctatacccg gtaatggcgc aggttgacct gcacttggcc aagtggattg
      961 cccgcaaaca caagcgagcg cgaggtagtc tggtgcgtgc attcgagtgg ttactgcgaa
     1021 ttcgtcacga ctgcccgacg ctgtttgcac actggtgctt agcttacgat acttgaatgg
     1081 ttcaacgaca agagccgtat gacgcgagag tgtcacgtac ggttccgtgg gagcgtaggg
     1141 gggcggtgcc cctgcgccac ccgatatccc ctttgagtgc ggttacgtcc acagaggtaa
     1201 atgccgctga cgcggcatct gtaatctgtt actttgtgta tatcatttgc tatatattgt
     1261 caatatgcta ttgttgtaaa gcaaatgata tacggaggct ttatgaaatc aggtgttcag
     1321 ctcaatctca gagcaagaga atcacagcgg atactcattg atgctgctgc agaaattctt
     1381 cataagtcgc gtacagattt cattctggaa atggcctgta aagctgctga agatgttatt
     1441 ctcgaccggc gggtgtttaa ttttaatgac aggcaatatg aagagttcat tgaaatgctt
     1501 gatgctccgg ttgcagatga tcctgccatc gaaaaattgc tggcaaggaa acctcaatgg
     1561 gatgtgtaac tgcaccagaa cctttatcct catttcatca ggtcgctgaa tttgtcagcg
     1621 gtgaagctgt acttgatgac tggttaaagc agaaggggct aaaaaatcag gcaatcggag
     1681 ccacccggac ttttgttgta tgcagaaaag gtacgcaaca aatagtcggt ttttactcac
     1741 tggccactgg tagtatcaat catacagaag ctacaggtaa ccttcggcgc aatatgccag
     1801 accccattcc tgtaattata cttgcacgcc tggctgtaga tgtgtcattt cgaggaaaag
     1861 ggcttggcgc ggatttactt catgatgcag ttcgtcgttg ttacagggtt gccgaaaata
     1921 tcggtgtgcg tgcaattatg gttcatgcgc ttacggaaaa cgcaaaacag ttttatattc
     1981 atcatggttt taagccatca aaaactcagg tgcaaacatt gtttcttaaa ctccctcaat
     2041 agggagtaat gccgatcagt taaggatcag ttgaccgatc cagtggctgt gtaagaatcc
     2101 ggaaacgctc actcgtttcc ggatttttct gtgcacagta aaagaccacc tcctgaggag
     2161 gtggcttttc agaatgcccc ctataa
//
LOCUS       DADMGC010000263         2159 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.266, whole genome shotgun sequence.
ACCESSION   DADMGC010000263 DADMGC010000000
VERSION     DADMGC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.266"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            1..147
                     /locus_tag="JNZ73_004207"
                     /pseudo
     CDS             1..147
                     /locus_tag="JNZ73_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
     gene            144..1214
                     /locus_tag="JNZ73_004208"
     CDS             144..1214
                     /locus_tag="JNZ73_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAY8734240.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECDTQISMVSGASIG
                     AFNGAIIATSPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGDNSGGLLSFTLAHTDAWRQQGYEDTMLTMEHIRKPLAARQVLSRSETVL
                     QKSLEITEEADLALRNAMARIK"
     gene            1350..2027
                     /locus_tag="JNZ73_004209"
     CDS             1350..2027
                     /locus_tag="JNZ73_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734241.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGK
                     FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARA
                     EQPGACPTTYELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDGPGGNVTPVTINGTV
                     WLQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVK
                     NYVAAVN"
BASE COUNT          551 a          502 c          581 g          525 t
ORIGIN      
        1 gactagtgca gtgaagagca gcatccggtg atctggtcaa ccacgcaggc tgcagaacac
       61 ttaccggttc gcatgcctcg cctctgctct gcagaacgcc tctctgaaag tatggtcgat
      121 gaaatatttc agaagggggc tgcatgagta cagaaatgaa aacggggctg gtgctgtccg
      181 gtggcggggc ggtgggcgct tatcaggcgg gagtggttaa ggcactggca gagtgtgata
      241 cacagatcag catggtttca ggggccagca ttggcgcatt caatggtgcc attatcgcga
      301 cctctcccga tctgtcagaa gctgccgtac gcctggaagc gctctgggat catctgggga
      361 ataatcaggt gctgtcggta aaccgattgg tttacttttc attgctgaaa aaattgttcc
      421 aggcaatgaa cctctgccag atccccggac gtgcaggagc actgcttacg acgcttcttc
      481 gccatatatc gatactcaac gggtttgaca atctgatggc tcagccgttg ttgtcagatg
      541 agcccctgac agcgctgttg gatcattatc ttgatactga tgctctggca gacgggctac
      601 cgctgtatgt gtcgctgtac cccacagaag ggggcatgca ggatattatt gactgcattc
      661 gtgctgaact gggtgccgga accacgaaaa acgccgtttt tcagcatatc cagagcctgc
      721 cccgcggaca gcagaaagag gctctgcttg cgtcagccgc gctgcccctg ctgttccgtc
      781 cccgtgaggt tcaggggaca atgttcggtg atggtggtat gggaggatgg cgaaatatgc
      841 agggaaatac ccctgtgacg cctctggtcg atgccggatg caatatggtg attgtgacgc
      901 atctgagtga cggttcttta tgggatcgcc aggcttttcc ggacaccaca atccttgaga
      961 tccgtccccg gaaaaggctg aaatatgcag gtgatggtga caacagcggc ggtctgctca
     1021 gtttcacgtt ggcacatacc gacgcctggc gtcagcaggg ctatgaagac acgatgctga
     1081 cgatggagca tatccggaaa ccgctggcag cacgtcaggt actgtcccgg tcagaaacgg
     1141 tgttgcagaa aagtctggag ataacggaag aggcagattt ggcactgaga aacgcgatgg
     1201 cccggattaa ataaagacgc tccaaagaaa cgccaccgcc tgtggtggtg tttttcctag
     1261 ctggtgttat tgaaaatatt ttcagatata tattacctga gagaaagttt tacccctcac
     1321 ctgtcaattc actcactgga aatttaatta tgcaattacc tgtaaagtta ttaatgagcc
     1381 ttatatctct ggtcagcgtt attgcacgtg ccgggaaata taaaaattat atccgggatg
     1441 aaataaaata ctggcgatat acatcataca agggggggaa atttccggaa ggtttcactg
     1501 atgagaaatt ttccagcgcc atttacaacg gaagaatatt tacaatgaaa cgtttacata
     1561 ccctgatgtt atttctggcg gttctgttta ctggctttaa cgtggaagca gcgagcgtga
     1621 aacaagcgct cagctgcgac ccaaacgccc gggctgaaca acctggagcg tgtccaacaa
     1681 cgtacgagtt gtacgaaggt gacgctgcct acaaagctgc gcttgacaaa gcattaaaac
     1741 cggtcggact gagcggcatg ttcggtaaag gcgggtatat ggatggccct ggcggaaacg
     1801 taacgccagt aaccattaac ggtacagtct ggctccaggg cgacggttgc aaagccaata
     1861 cctgcggctg ggactttatc gtaacactct ataacccaaa aacccatgaa gtcgttggct
     1921 accgctactt tggtttagat gacccggcct acctggtttg gttcggcgaa attggcgtgc
     1981 atgaattcgc gtatctggtg aaaaactacg tagctgcggt taactaattt aaggagtctg
     2041 tcatgaaaac ttttatcaaa actttactcg ttgctgtaac tattctgttc tctgtcttcg
     2101 ctacggcgaa acaagtaaaa ctgccaaaca acatcaaata cgttaatact acagaggcg
//
LOCUS       DADMGC010000264         2157 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.267, whole genome shotgun sequence.
ACCESSION   DADMGC010000264 DADMGC010000000
VERSION     DADMGC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.267"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            83..463
                     /locus_tag="JNZ73_004210"
     CDS             83..463
                     /locus_tag="JNZ73_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HAY8734242.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(504..1454)
                     /gene="corA"
                     /locus_tag="JNZ73_004211"
     CDS             complement(504..1454)
                     /gene="corA"
                     /locus_tag="JNZ73_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HAY8734243.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            1824..>2157
                     /locus_tag="JNZ73_004212"
     CDS             1824..>2157
                     /locus_tag="JNZ73_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734244.1"
                     /translation="MAYRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGDIYDESYAPLG
                     LYIENGQRK"
BASE COUNT          582 a          506 c          514 g          555 t
ORIGIN      
        1 tttttgattt gttatttttc cactttcagt atgctaatgc ttgcacctac tgattcatca
       61 aaacaatagt tatggagtat ttatgagtaa ggaatatatg aacgatgatt cattaagtga
      121 aaaatggaaa taccgattta atttttatga tcaacatggc tttcctggat tctggggagc
      181 aacaccagaa tataaagcag catttaaagc attaaaagta agacaacgat taactattca
      241 aatgaatttc attgctttct tctgttcctg gatctattta tttgttctgg gcttatggaa
      301 aaaagcaatt atagttctat tacttggcat tctatctcta tttgttggtg cattaattgg
      361 cgttaacatt ctgggaattg ccgtagcagc ctatgttgct gttaacacga acaaatggtt
      421 ttatgagaaa gaggtaaaag ggttaaatac ctggagcctt tgaataacag gtggcttagc
      481 caggctaagc caccgccttc tttttacaac cagttcttcc gcttaaagta cagatacggt
      541 gccaggcccg cgaggatcat aaagataatc gcgccagggt agccgaaact ccacttcagt
      601 tctggcataa actcaaagtt catcccgtag ctggaagcaa ccagcgttgg cggcaggaac
      661 acgacggata ccaccgagaa aattttgatg atgcggttct gctcgatgtt gataaaaccc
      721 attgccgcct gcatcaggaa gttcaccttc tggaacaggg attcgttatg cggcagcagg
      781 gattcgatat cgcgcaggat ttcacgcgcc tgctccagtt gcccacccgg taaacgcgct
      841 ttacgcacca ggaagttgag agcgcgctgg gtatccatca gacacaggcg aactttccag
      901 ccgatatctt ccagttccgc cagcgtggag agcgcctcgt cgtactcatc gccctgatgc
      961 ccttccataa tcacccggct caactgctcc aggtcgctat agatattttc aatttcatct
     1021 gccaactgtt cgattttggt ttcgaacaga tccagcagca actcgtaggc gttaccgtct
     1081 accatcgact ggctacgggc acgcatacga tacagacgaa aagcgggcag ttcacgctca
     1141 cgcagagtaa acagacgacc atcacggatg gtaaatgcca cagtggagtt accggcgtga
     1201 tcttccgcat cttcaaagaa gaagaaggag tgaatatgca ggccgtcgtc gtcttcaaag
     1261 aaacgtgccg atgcttcgat gtcttccagt tccgggcggg ttgccaggct ctggccaagt
     1321 tcagattgta cgcgcagtcg ctcgtcgtcg tccggttcga caagatcaat ccatactgca
     1381 tttacaaggg gttgtgactc ttcgacttcc agccgggtca gtcggttatt ttccagttga
     1441 aatgcgctca gcatgaccgg gactcccaat gcttaaaata tcggacagtt cggtgggcac
     1501 acagaaacaa attgggtttc agaccattaa acagcctgac tcagcgcgac gggaaaaatt
     1561 gaggtcgctg acaaccgcta aggctatcag caaaaaggga tagccttagg agttgatcct
     1621 ggatgacagg atagtgagcc agtatctact gggtgtgtcc aaggcgaatg tcctcttaga
     1681 gtgatcgtgc gcgcatgtta cgccagcaaa attttgccgt caacacgcaa cagaacacca
     1741 cagaacaata gttgaacttc gcgctttttg gtactcgtca aggtcgctaa caggataaaa
     1801 aaatcccaca tccaggacct ataatggcgt atcggctact cattggtaaa ggaatgatca
     1861 ccttgaatct caaacggatt tttctcaccc tcaccttact tcccttgttt gctgttgccg
     1921 ctgatgattg cgcactctca gatccgacgc tgaccgtaca ggcgtatacc gttaatcctc
     1981 aaacagagcg ggtgaaaatg tactggcaaa aagccaatgg cgaagcgtgg ggaacgttac
     2041 atgctctgct ggcggatatt aatagtcagg gtcaggtgca gatggcgatg aacggcgaca
     2101 tctatgatga aagctatgcg ccgctcggtt tgtacatcga aaacggtcag cggaagg
//
LOCUS       DADMGC010000265         2151 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.268, whole genome shotgun sequence.
ACCESSION   DADMGC010000265 DADMGC010000000
VERSION     DADMGC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.268"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="JNZ73_004213"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="JNZ73_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HAY8734245.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="JNZ73_004214"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="JNZ73_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1982)
                     /locus_tag="JNZ73_004215"
                     /pseudo
     CDS             complement(1292..1982)
                     /locus_tag="JNZ73_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1905..>2151
                     /locus_tag="JNZ73_004216"
     CDS             1905..>2151
                     /locus_tag="JNZ73_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734246.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAI"
BASE COUNT          634 a          395 c          490 g          632 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaacacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt gagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttat gttgtaattt
      901 atttatggag gttttctatg gaattctttt ttattgtgac tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aatccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgata aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt aaggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt accgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc
     1741 cgtatgacgg tattgatgcc gattttcagt gttctggcgg tataacagcc ccctgcgccg
     1801 ttatgtgcca tttcaacgat ctgctcctta acgccgagta ttctggcttc ataagagtaa
     1861 gtgagctgga aaacacgttt acatgagcga caacggaaac gctcatgccg ggaacaacta
     1921 cggccatgtc gataaacttc atcggaatta cagcgcggac aatgaacagt aacggtagtc
     1981 atggaaaacc tcaaatacca gtagattacc agaaacttct atcgattgaa gacattacct
     2041 tttgccgcaa taggtggggt aacattggtg aaaacgcatt acgccgggtg gcagttggaa
     2101 aaaaattatc ttttttcggc tctgatcgtg gtggagaaaa tgcggcaata a
//
LOCUS       DADMGC010000266         2148 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.269, whole genome shotgun sequence.
ACCESSION   DADMGC010000266 DADMGC010000000
VERSION     DADMGC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.269"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..606
                     /locus_tag="JNZ73_004217"
     CDS             <1..606
                     /locus_tag="JNZ73_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY8734247.1"
                     /translation="AKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEA
                     GTVFIHINEDDSSLPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAM
                     VFNGLKLMKNGYGEQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYA
                     QAIMGHSSGSITYDRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            725..1058
                     /locus_tag="JNZ73_004218"
                     /pseudo
     CDS             725..1058
                     /locus_tag="JNZ73_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708644.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          587 a          476 c          531 g          554 t
ORIGIN      
        1 gctaaagcgt actcgcagaa gacctcaggt aagccatacc attattacgt caccgctctg
       61 gctgcgatta caggtgcgag acttaacgag gtggcccaac ttcaggttaa agatgtcagg
      121 accactgagg ctgggaccgt ttttatccat atcaacgaag acgatagcag cctgccgggt
      181 aagagtatca agaacgccca cagtgaccgc tgtgtcccgt tggtagatgg tgcttatggt
      241 tttgtattgg ctgactttat gtcactcgtg gaagaccgta gaaagactga gggtgataat
      301 gctatggtgt tcaatggcct caagctaatg aagaacggct acggtgagca ggttagcaag
      361 tggtttaacc gaacgttgct gcccaaagtc ctcgctgacc gtagcggctt agcttttcac
      421 tcgtttagac atacagttgc gacccagtta aaacaacatg gggtcgagtt agcgtatgct
      481 caggcgatta tgggtcatag ctcagggtct ataacttacg accgttacgc gaaagaggtc
      541 gaggtggaaa cgctgaaaga aaaattagcg gagtcattgt cagtaaaaaa aatagacgga
      601 aagtaatcgc atagggatag cggaatgtga ggacgtaatg gtcaaagaat ttttagttag
      661 caaagaaaat aagccttcat atgaatgtat gaatactgct gaaacttgtt tggggctggc
      721 tggaatggct tcggaagatt ggcaacgtga gcatcattgc cgcacaggta taatatttac
      781 tgcttttgct attgaggcaa tgttcatctt ctatagaaaa caggttgacc ccggctacga
      841 taagacgcag aaagagtgca gaaagacaat gcacaagaat acgttaaaat tgtgcggtat
      901 aaacaattat atggggacaa aaccttacca aattatcaaa gagtgtcttg aggtaagaga
      961 cgctattgct cacggcgatt cgtatacttc gagctttaat ttctcggcgg atcatttgga
     1021 taaccaagac gatattgcaa aaagtgtcct ggcggtaaac tctgaacaat ttcggggatt
     1081 aactgttgag cgccttgcaa aatacattga aatggcaaag catattgacc atgagatttc
     1141 tgataatggt tacaggcctt ctgaaggtta ccttccagca gccgagcgca gaagtctgat
     1201 gttagctttt ggcgtcagtg gtgtgtcaat gtggcccagt gcttgagcga taatacacct
     1261 cactatagag gaatggaccc ttaactatca ctatagggga gagaccctaa gtccccacct
     1321 taatagcagt cacgacccat acacacattc attaagtctc aggcttccgg tagcggaccg
     1381 attggaaacc atccgcagac gcctagtgtt aaatcacctt ggcctgagac cattcctttt
     1441 aacgtaagct atcttccctt ttgacaggcg gggagcagta gaccacatcc gactttaagt
     1501 tggctctgtg ataatcgctg cgcggataac tgaagaacca agacaccatt actgaggtct
     1561 gccatccatt tgtggacagg cggaaactca aagaggttag atcagtggtg tctgaggttg
     1621 gagctttgag tcttgaccag caggctggag gagaccatga gtttatcttg ttatggattg
     1681 tgttatcaca attccaataa gaagaacttt aagtccagcc tccactcccg ccaacccgaa
     1741 gtaaaattat ggtcgctatg atgtggtctc catgcttcgg gtcttactgg aagtactgaa
     1801 cactttcgcg tcaagttctc cgaagttcat tcctgtttga ctaaactatc actataggga
     1861 ccatatctcg cctctgacct cctgagagac ggcaagcgag aaaccgtggt ccacgctagc
     1921 aagctagcca ccagcctaag gcaagacagc ctctggtcag cctgtgtgtt ctttggtctc
     1981 gtgacttaag tctatcacca cacacgctcg gacatgctgt cgaaccttca tctctatggt
     2041 catcgcagac cactaacccc aattacctca agtcattctg tttgcgtcct gttgcctgct
     2101 ggtctccctt tacgtggggg cttgagtagg tcagtggggt gcgacttc
//
LOCUS       DADMGC010000267         2117 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.270, whole genome shotgun sequence.
ACCESSION   DADMGC010000267 DADMGC010000000
VERSION     DADMGC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.270"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..256)
                     /locus_tag="JNZ73_004219"
     CDS             complement(<1..256)
                     /locus_tag="JNZ73_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734248.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSM"
     gene            281..520
                     /gene="relB"
                     /locus_tag="JNZ73_004220"
     CDS             281..520
                     /gene="relB"
                     /locus_tag="JNZ73_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HAY8734249.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            520..807
                     /gene="relE"
                     /locus_tag="JNZ73_004221"
     CDS             520..807
                     /gene="relE"
                     /locus_tag="JNZ73_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HAY8734250.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            879..1034
                     /gene="hokD"
                     /locus_tag="JNZ73_004222"
     CDS             879..1034
                     /gene="hokD"
                     /locus_tag="JNZ73_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HAY8734251.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1252..1502
                     /locus_tag="JNZ73_004223"
                     /pseudo
     CDS             1252..1502
                     /locus_tag="JNZ73_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1569..1847
                     /locus_tag="JNZ73_004224"
     CDS             1569..1847
                     /locus_tag="JNZ73_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734252.1"
                     /translation="MARNAKYYNHDNSTVLAHTHERYSHAFKSDWYQHPSCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSEQPPRPSRTFQQRIWR"
     gene            1849..>2117
                     /locus_tag="JNZ73_004225"
     CDS             1849..>2117
                     /locus_tag="JNZ73_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734253.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRLLVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRH"
BASE COUNT          549 a          483 c          551 g          534 t
ORIGIN      
        1 acatgctaaa gcaaaaaata gatgaataag ttgagttgtg catatgtagc ctgaccgtca
       61 caaagtatat ggtgtctgta ccagtaagat gatggccgga ctctttaaaa aagagctgac
      121 ctgcacaata caggatggac ttagcaatgg ctgctcctgg cacaaagcgg acagtgatca
      181 ccgttcttac gactactttc tgacttcctt cgtgacttgc cctaagcatg ttgtagtgcg
      241 atacttgtaa tgacatttgt aattacaaga ggtgtaagac atgggtagca ttaacctgcg
      301 tattgacgat gaacttaaag cgcgttctta cgccgcgctt gaaaaaatgg gtgtaactcc
      361 ttctgaagcg cttcgtctca tgctcgagta tatcgctgac aatgaacgct tgccgttcaa
      421 acagacactc ctgagtgatg aagatgctga acttgtggag atagtgaaag aacggcttcg
      481 taatcctaag ccagtacgtg tgacgctgga tgaactctga tggcgtattt tctggatttt
      541 gacgagcggg cactaaagga atggcgaaag ctgggctcga cggtacgtga acagttgaaa
      601 aagaagctgg ttgaagtact tgagtcaccc cggattgaag caaacaagct ccgtggtatg
      661 cctgattgtt acaagattaa gctccggtct tcaggctatc gccttgtata ccaggttata
      721 gacgagaaag ttgtcgtttt cgtgatttct gttgggaaaa gagaacgctc ggaagtatat
      781 agcgaggcgg tcaaacgcat tctctgaacc aaagcatgac atctctgttt cgcaccgaag
      841 gtgacacttc tgctttgcgt tgacaggaga agcaggctat gaagcagcaa aaggcgatgt
      901 taatcgccct gatcgtcatc tgtttaaccg tcatagtgac ggcactggta acgaggaaag
      961 acctctgcga ggtacgaatc cgaaccggcc agacggaggt cgctgtcttc acagcttacg
     1021 aacctgagga gtaagagacc cggcgagtga gaaatccctc gccacctctg atgtggcagg
     1081 catcctcaac gcacccgcac ttaacccgct tcggcgggtt tttgttttta ttttcaacgc
     1141 atttgaagtt ctggatggtg ccggaataga atcaaaaata cttaagtagc gcgcagggat
     1201 aaaagggatg gaccccgaac aggggagtgc tacttatctg gaaggattct gatgatgaac
     1261 atcgaagaac tgcgtaaaat tttttgtgaa gatggcctct atgctgtgtg cgttgaaaat
     1321 ggaaatattg ttagtcatta ccgcattgtg tgtttgcaaa aaatggggct gcgttaatta
     1381 attttgtgga tgcccgagtg acggacggat ttatcttgcg cgacggtgag tttgtcactt
     1441 cattacaggc attgaaagag atcggaataa aagctggctt ttctgctttt tcagaagaat
     1501 aaactcatct acaatcttgc gcggggctga actcccgctg agtaacaccg tgccaccgga
     1561 gaaaaccgat ggcacgcaac gcaaaatatt acaatcatga taattcgacc gttcttgccc
     1621 acacgcacga gcggtattct cacgcattta agtcagactg gtaccagcat ccctcatgca
     1681 ctgaagaaca ggccgaatgg ctcattcagt gttaccgcag gcgcggatgc gaggttaaaa
     1741 aagcccttag cctcgactac cgtcactgga taatctccgt caggctccct tactccgaac
     1801 agccaccgcg tccgtcccgc acattccagc aacggatctg gaggtaacgt gcgggtatta
     1861 cttcgacctg ttctggttcc ggaactcggg ctggtggtcc ttaagcccgg tcgtgaatcc
     1921 atgccggtat tccacaatac ccggttactg gtggagccgg aaccgaaaag catgcgtaat
     1981 ctgccgtccg gggtcgttcc tgccgctcgc cagccgctgg tggaagacaa aacattgctg
     2041 ccgtttttca gtaacgcacg ggtgattcgt gctgctggtg gtgctggtgc attgtctgac
     2101 tggctgttgc gccatat
//
LOCUS       DADMGC010000268         2103 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.271, whole genome shotgun sequence.
ACCESSION   DADMGC010000268 DADMGC010000000
VERSION     DADMGC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.271"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            114..1151
                     /gene="curA"
                     /locus_tag="JNZ73_004226"
     CDS             114..1151
                     /gene="curA"
                     /locus_tag="JNZ73_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HAY8734254.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWGLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVMGVAGGAEKCRHATEVLGFDVCLDYLADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1349..2014
                     /locus_tag="JNZ73_004227"
     CDS             1349..2014
                     /locus_tag="JNZ73_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY8734255.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          576 a          449 c          564 g          514 t
ORIGIN      
        1 ttcctcgatg aaattattgc gtgttgcaga ggggatgtga cggctgcaaa caaaaccaat
       61 cccgtgagcc tgctactatt gatggattca tgccaaaagc acaggagagc attatggggc
      121 aacaaaagca gcgtaatcga cgttgggttc tggcctcgcg tccacatggc gcaccggttc
      181 cggagaattt ccgtcttgaa gaagatgatg tcgccacacc gggtgaagga caggtgttac
      241 tgcgcacagt ttatttgtcc ctggacccgt atatgcgtgg acgtatgagc gatgagccat
      301 cttattcacc gccggttgat attggcggcg tgatggtcgg cggtacggtg agccgtgtcg
      361 tggagtcgaa tcatcctgat tatcagcctg gcgactgggg gctgggctac agtggatggc
      421 aggactatga catatccagt ggtgatgacc tggtgaaact tggcgatcat ccgcaaaatc
      481 catcgtggtc gctgggtgtg ctggggatgc caggctttac cgcttatatg gggctgctgg
      541 atatcggtca gcctaaagag ggcgaaacgt tggtggtagc tgcggcgaca ggaccagtgg
      601 gggcgacggt ggggcaaatc ggcaaactta aaggttgcag agtgatgggg gtagccggtg
      661 gcgcggaaaa atgccgccat gctaccgagg tgttaggctt cgatgtttgc cttgattacc
      721 tcgcggacga ttttgccgaa caactggcga aagcgtgccc aaaaggtatt gatatctatt
      781 atgaaaacgt gggcggtaag gtatttgatg cagtgctacc attacttaat acatctgcgc
      841 gcattcccgt ttgtggctta gtgagcagct ataacgctac agagctacca cccggtccgg
      901 atcgtttacc cctgttgatg gcaacggtgc tgaaaaaacg tattcgcttg caagggttta
      961 ttatcgctca ggattatggt caccgcatcc atgagtttca gaaggagatg gggcaatggg
     1021 tgaaagagga taaaatccac taccgcgaag atattactga tggtttagag aatgcgccac
     1081 agacgtttat cggcctgctg aagggtaaaa acttcggcaa agtggtgatc cgcgtcgcgg
     1141 gtgatgatta aagtttcaac ggcggcgtaa gccgccataa aaatccaggc aaaaagctta
     1201 ttttcagctt taattaacta accacgtcat tgttctgctg ctaataacaa caaaggtggg
     1261 tggtttatga ttattcactt taatacacta ggtgaactcc ttctgccatg caggcagggt
     1321 tggacagaaa acacgcgaag gaattaaaat gcctggaacg ggaaaaatga aacatgtcag
     1381 tttgactctg caggttgaga acgacctgaa acatcagctt agtattggcg cattaaaacc
     1441 tggcgcacgc ctgattacta aaaatctggc ggagcaatta ggtatgagta ttacacctgt
     1501 gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt tctgtcgcac ctgcacaagc
     1561 atttacagta ccggaagtga ggcaaagtca gttagacgaa atcaatcgga tccgctacga
     1621 actggaatta atggcagttg ccctggctgt tgaaaacctc tcctcgcaag acatagcaga
     1681 acttcaggaa ttgctcggga aattacagca ggcgcaagaa aagggcgaca tggaacaaat
     1741 cattaatgcg aacaggctat ttcgcttagc gatttatcat cgctcaaata tgcccatcct
     1801 gtgtgagatg attgagcaac tgtggatcag gatgggacct gctttacatt atctttatga
     1861 agcgattaac ccagcggaat tacgggagcg catagaaaac tatcatctat tactcgctgc
     1921 gttaaaagca aaagacaaag agggatgcaa acattgtctt gctgaaatta tgcaacaaaa
     1981 tattgctatt ttataccagc aatacaatcg ttaaattaaa tgcccggcaa ataccgggca
     2041 tcaagtaatt actcaaatcg ccacgcaata ttcatgccga caccataatt tcgtccgggc
     2101 gca
//
LOCUS       DADMGC010000269         2077 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.272, whole genome shotgun sequence.
ACCESSION   DADMGC010000269 DADMGC010000000
VERSION     DADMGC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.272"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(195..428)
                     /locus_tag="JNZ73_004228"
                     /pseudo
     CDS             complement(195..428)
                     /locus_tag="JNZ73_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(524..2047)
                     /locus_tag="JNZ73_004229"
     CDS             complement(524..2047)
                     /locus_tag="JNZ73_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY8734256.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          622 a          459 c          442 g          554 t
ORIGIN      
        1 acatcaaaat tattaatagt tgccatggta attatttcgg tgatatataa aaacaactgt
       61 gataacacca aggttgacaa ctatttgcgc aataattcct ttccttcaat gatccaggcg
      121 ttcactgcca gcaacccatt cagccgcata ctgacagtga gaaactgaaa gattattgct
      181 gatccctgtg gctgacgagt ctttctctta aatcgattaa ccagattgat cgccagccaa
      241 acaagggcat taagcaaatt tatcgtcaca agcacttctc ccagcccggt actgggtttt
      301 gcaataagga tgaccaggag ggtgatcagc ataaatgcaa tggaaaagat gatatagaga
      361 gtgaaataat ccagattatt actattgttc atgctactaa tggcaatcct gtcaaaactt
      421 tcaaacaatt atagcaaaca aaataatgaa caatagtaac aagaggcaac gagctaatta
      481 gctgtctata attacaccgc actatggcct tactgatcaa ttttcactca accacaacct
      541 tcaccttcgg tttagaaagg ataattttga ccaggtcgat gtatgtaaca ggtttataga
      601 aataataacc ctgctgaaat gtgatattat tttggttcag atagccaagt tgttctttcg
      661 tttcgacacc ttcagcgacg atactgatcg aaagtttacg cgccagatcc aatacacaat
      721 ccagaattcg ggttgattct gggtctgcgt ttacgcggcc aacgaaactg tgatcaattt
      781 tgatataatc aatatgcagg tcatgaagat aagaaagccc tgagtagcca gtaccgaaat
      841 catccagcgc gatgacaaaa ccattttcat gcagaatgtt caaccgctga accagacttt
      901 catcaacatt caatggctca cgctcggtga cttccagaac aaggtttaaa tcgcggcggg
      961 taaaactgtc acggaaattt aaacactcgt cgacaaacgt cggcgaaata atatgcgaag
     1021 cgctaaaatt aattccaata tgaaaacctt ccggcagttt actcgcgata gcgttcatct
     1081 gtctggcaac ctgattcatc aggctttgtg taagcggaac gattaatccg gatttttcag
     1141 caagtggaat aaatgccgcg ggtgaaatat atccaccgtg aggttgtttc cagcgggcta
     1201 acacctcaac tccccgcaat gtcccttccc gaccatttac cacaggttgg taaaaaggga
     1261 cgatctcccc attatttatt gcccgtcgca ggatctcttc aggcgttgcg cttttattga
     1321 aatatctatc aagcaggaag gcagcggcgc aggcgattaa cagaataaat attaaaatcc
     1381 caaagccttt atcgataagt ctgtagaagc taaagagtgg cggcgggtta aagataatag
     1441 tgaaatgata ttttgtggag tgaaccttct cggtataggg ataatgtcca ttaagcgtca
     1501 tcacatcacc cgttggtcca ataatgtcat ccgccacgcg taatacatac cttacccctt
     1561 tcaagggtac attaagcgcc ccgcgaatat gttgatcact tatcgtaacc aaaatgcgcg
     1621 tatctgcaaa ttggctctga tagagcagga taggtaatct attaacggtg tcgattgccg
     1681 gagccaacag taaattactg tccgggacaa caggaatacg agacagcagg acccgattcc
     1741 caggcaggga tgtacaccag actattccct gtttgagaat gatgattgtg cgcaggtgag
     1801 gtttcagagc tgcttcagtg ccaagctgat attgctcctc gaggtcgcat tccttcccgg
     1861 caatgttaac cgctgtttga gtcgcctgtg acgcttcttc aagtatatga ttgatattgt
     1921 tggcggcata tcttgctccc gccaggtact ctgcttttgc ggaataccag agctggatgt
     1981 tcaaaatagc gacaccggta ataaacaagc atatcgcaac gaggatgggt atgagtgtat
     2041 tgcgcatttg cttgtcccgg ccaaagggtt taataca
//
LOCUS       DADMGC010000270         2049 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.273, whole genome shotgun sequence.
ACCESSION   DADMGC010000270 DADMGC010000000
VERSION     DADMGC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.273"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..168
                     /locus_tag="JNZ73_004230"
     CDS             <1..168
                     /locus_tag="JNZ73_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HAY8734257.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGIL"
     gene            153..791
                     /locus_tag="JNZ73_004231"
     CDS             153..791
                     /locus_tag="JNZ73_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAY8734258.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADTGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1019..1420
                     /locus_tag="JNZ73_004232"
     CDS             1019..1420
                     /locus_tag="JNZ73_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734259.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEISLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSSGGGR"
     gene            1431..1905
                     /locus_tag="JNZ73_004233"
                     /pseudo
     CDS             1431..1905
                     /locus_tag="JNZ73_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          620 a          365 c          480 g          584 t
ORIGIN      
        1 tccgctattg ccagtacacc tcacccgtcc tggcgacgta tctgtaagac actcataaag
       61 aatgattttt ggtgccggac tctctccttc agtccgaaca aaccccggca ctatgaacgt
      121 tacctgcagc gtatgaagga aaggaggaag gaatggggga ttctgtgaca ccggagatac
      181 ttggcaacat gattcgtcag tatttcagtc aggtacgaac tgaaaaagag agcatacagg
      241 ctctgaatca tctgcgcaga gtgcttcatg aggtcagtcc ttttgcacag gagcctgtgg
      301 actgtgtgtt gtgggtgaaa gcggatgagg tggtggccaa cgattacaac cctaatgtca
      361 tggcactagg tgagaaaaaa ctactgaaac agtctctgga gaaagatggt ttcacacaac
      421 cggtggtggt gtcagaggag aagaatcatt atctggtggt ggatggtttt catcgtcagt
      481 tactggggcg ggaagcagat acaggaaaac gcctgagggg ctggttgcct gtagcctgta
      541 ttaatccgga gagaaaggga caggccgcac gtattgccgc gaccattcga cataaccggg
      601 ccagggggaa acaccagatc actttaatgt ctgatattgt cagggatctt tcacgtctgg
      661 gctggacgaa tgaacgtatt ggtacagagc tgggaatgga ccaggatgaa gtgctgaggc
      721 tgaaacagat tagcgggctg acagagttgt ttcaggaaga ggatttcagc tcggcttgga
      781 cggttcgttg atgttgttaa atatgggcgg tatttctgaa agttgatact ttgggtagaa
      841 aaaatgatac cgaaaactat ggaggatgtg cttgtcaggt gactggacac cataggtctt
      901 aggctttgat aacgggataa aatgagcggc ttttctcata ccatcaggtg tgatgatggc
      961 gcagatttac aaaatctgat tgattctaaa gatgtataag taaaaatcaa gctttgatat
     1021 gaaaaaacag atatttatta ataataaacc tcctgtagtc ccttactcag ggacgcacgc
     1081 gaaaatattt aaatacattg aaatctctct gccgtttttc tactttatct atacatcagg
     1141 cgaaccattc cacatatctg tccaaaatac tgttatttat gtatcaaaat acaatggtat
     1201 atttattaat aaattagttc ctttttctct tttatttgac agggatatat ctgttcttca
     1261 aagaagagat atttgtgttg tgcgatttac gtcagaggaa ataagtgaac ataacgtttt
     1321 atttgatcat gacattgaac gattaaaaaa aatttccaaa gctcagctta tatctcccga
     1381 ttatgttttg attgattttt ctagtggggg ggggagatga accctatgca atgtcctggt
     1441 taatatctca gtgtgctcat cagtgcactg ataataaaaa gacggagaca gatgccattt
     1501 acgataaagt tcggagttct tatttgcttt catgtattct gaaaaaaaac aaaaatgttg
     1561 ggcttattct tcatgcacca tcatttgttt ctgtgtctga aaaaaatcgc cagaattgtg
     1621 atggcaaatt actcccgtaa ctggagtaat tccgagttgg ccagtgcggt tttaatgagt
     1681 gaatcttctt taaaaagaag aatgtacaaa gaggtcggtt ctataagtac atttgtacat
     1741 aaaataaagc ttacagaagc tatcaggaaa ttacgaagga caaatactcc catttctgtc
     1801 atatcatctg agttaggtta cagttcacca tcgtattttt ctaaagtttt ctttaaatat
     1861 ttaaagacat accctcaaaa cataaggaag aaaaatggtc ggtaatgtca ccaattagga
     1921 ggacttgcag agccgctttt ttacgatact gagatcaaac agacataatc tatgcggaga
     1981 tttacagcag ggcatggttc acaaatctga cagcgatgaa ttatcggcac tgcgggcaga
     2041 aaatgctcg
//
LOCUS       DADMGC010000271         2037 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.274, whole genome shotgun sequence.
ACCESSION   DADMGC010000271 DADMGC010000000
VERSION     DADMGC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.274"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            396..649
                     /locus_tag="JNZ73_004234"
                     /pseudo
     CDS             396..649
                     /locus_tag="JNZ73_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
     gene            806..>2037
                     /locus_tag="JNZ73_004235"
     CDS             806..>2037
                     /locus_tag="JNZ73_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8734260.1"
                     /translation="MRQLVISITEGLNMSLFTEPKEIERLPSEEIERLYPVLRYRVFI
                     SIFLGYMGYYFVRNTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSK
                     VFLSLGLFLSGLVNFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEH
                     RERITWATLWNLSHNVGGALAPVLIGFSFGFFGDSALEHARAAFIFPGVLCMAMSVLI
                     YFIQVDRPVSVGLPPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCI
                     YGSFVYILRYGIVSWAPKFLSDSLDVGGKDMGKLASMGGFSVFEIGGVAGMLLAGYLS
                     VRLFRNSKPLTNTLFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIG
                     LYSMELVPKEAAGAASGLS"
BASE COUNT          537 a          408 c          461 g          631 t
ORIGIN      
        1 caatttcgca cgtataaaca tctttgacgc cagcgagata cagccatcct tcccgggtgg
       61 caacatacgt tatatctgcg acccagacct gattaggggg gttggggagc aatggaacag
      121 taaacgccgt taagaagtca atttaaatag ccaaaaatta actagccaat ttacattgca
      181 ggttgtaatt tttaaccacc ttttttacga tagccaaatt acctataccc atcccggccc
      241 tgccaagcca tacccgctat cggcgatgaa aatgcttttg tgcgcggtcg gagtggtccc
      301 gacgagggtt ttacccgaag tcggggcgta tctccgcgtt agcgggccgt gagggccgct
      361 tacgagcgtg tatgagagac gtacagtggt tttgtccgct cacaccagta catacaggta
      421 cggaaataaa accagtcgaa atgccggtaa cgacgattac tcctgtctct gatgccggtg
      481 gactacaaga ttttatttac tggcgtcctg atgcggacag gactggtatg gaacctgttt
      541 atgtgatact taatgatcct ctggattccg ggcgatttac gcgtaaacaa ctaaataaaa
      601 atatcttaaa catgcaagta attttggtat tctcgataca agaataaata gtagtaacta
      661 ctatagttgc aaataatgtt aaaaacattt ctatgatcat ctttacattt tttatgattg
      721 acaattgttt ttggttaaaa aaagacaact aatgagtgtg ataatgcaat actttgagta
      781 attatataat gataagtaaa caggtttgcg acaattggtt atcagtatta cagaaggatt
      841 aaatatgtca ttattcacag aacctaagga aattgaaaga ttaccctctg aagagattga
      901 acgattatat cccgtcctgc gatacagggt ctttatcagc attttcctcg gatatatggg
      961 gtattatttt gtccgaaaca ccaccagtgt gttatccggt gtgctgcaca tgtctgcaac
     1021 cgagatcggc attatctcct gtgccggttt tttatcctat ggtatatcaa agtttgtttc
     1081 cggacttatt tctgacagaa gtaattcaaa agtatttttg tctcttggtt tattcctgtc
     1141 cggactggta aattttctga taggatatat tcccgggatc atcacatccg ttacgttatt
     1201 ttcaacaatg tatttactta acggctggat ccaaggtatg ggatatcctc ctggtgccaa
     1261 aacactggtg ttctggtatg aacatcggga aaggatcact tgggccacac tgtggaattt
     1321 atcgcacaat gtcgggggag ctctggcccc cgttctgatt ggtttcagtt ttggtttttt
     1381 cggcgacagt gctctggagc atgcccgggc cgcatttatc ttcccaggag tactgtgtat
     1441 ggcaatgagt gtgctgattt actttattca ggtggacaga cctgtatcag tggggcttcc
     1501 tcccattgaa gaatggaaag gtaacgtcgt ttcacacccg gctaaaggcc gtgaacaagg
     1561 tccgagactc tccattcctg atatcatcag aaaacatatc atccgaaata ataaactcat
     1621 ttactgctgt atctatggtt cgtttgttta tattctgaga tacggtattg tttcctgggc
     1681 tcctaagttt ttaagtgaca gtctggatgt cggtggtaag gatatgggaa aactggcatc
     1741 aatggggggg ttttctgttt ttgaaatcgg tggtgtggct ggtatgcttc ttgccggata
     1801 cctgtctgtc agactgttca gaaactcaaa accattaaca aatactttat ttctggcact
     1861 taccattatt ttattaatcg cgtactggta cgtgccatca ggcaatgaat atctctggct
     1921 aaactataca atccttattc tgttggggct ggcagtgtat ggtccggtga tgtttattgg
     1981 tctttattca atggagcttg ttcctaaaga agctgccgga gccgcatcag ggctgtc
//
LOCUS       DADMGC010000272         2032 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.275, whole genome shotgun sequence.
ACCESSION   DADMGC010000272 DADMGC010000000
VERSION     DADMGC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.275"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..362)
                     /locus_tag="JNZ73_004236"
     CDS             complement(<1..362)
                     /locus_tag="JNZ73_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002443793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8734261.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /locus_tag="JNZ73_004237"
     CDS             complement(429..1643)
                     /locus_tag="JNZ73_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HAY8734262.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          632 a          493 c          414 g          493 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt gcgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttccac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttaaata atcacataac aaactccatt aatcattgaa
     1861 aatgagcaca gaaatagaaa ttaattatat tcgattaatt aaaatgtgta ttgcttgatg
     1921 tatttaaaaa tattattttc atggtgagag tggaggtaaa gaagacacaa taataaaaag
     1981 gaatgtgaca aataatggtt ctggttgggc gttaaattta aggtcaccag aa
//
LOCUS       DADMGC010000273         2008 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.276, whole genome shotgun sequence.
ACCESSION   DADMGC010000273 DADMGC010000000
VERSION     DADMGC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.276"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..132
                     /locus_tag="JNZ73_004238"
     CDS             <1..132
                     /locus_tag="JNZ73_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HAY8734263.1"
                     /translation="ASANNIKKRKLYIRSLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            132..302
                     /locus_tag="JNZ73_004239"
     CDS             132..302
                     /locus_tag="JNZ73_004239"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734264.1"
                     /translation="MTKKIKCAYHLYNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            377..652
                     /locus_tag="JNZ73_004240"
     CDS             377..652
                     /locus_tag="JNZ73_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734265.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            752..>2008
                     /locus_tag="JNZ73_004241"
     CDS             752..>2008
                     /locus_tag="JNZ73_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAY8734266.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEP"
BASE COUNT          624 a          462 c          461 g          461 t
ORIGIN      
        1 gcctcggcaa acaatattaa aaagcgaaaa ctttatattc gtagcctgac tacaaaaaca
       61 tgcatttctg actgcatgat taaagtcttc ctcggtcgtg atggtttacc tgtaaaggcg
      121 gagtcatggt aatgactaag aaaataaaat gtgcttatca cctttacaat aaagaaattg
      181 aagaaagcaa aatcattaca agaccacttc atttcatgcg tggagttata ccaacgacgg
      241 aaatgaaaaa atattgtagt gaaatctgtg ccgaaaaaga ccagatggca cacgaacttt
      301 aattaactga ctatccgaaa ctgaatttat gccagcaatg gcagggattc gctcaacctt
      361 aattaaggag aaaaacatga ttaccagtta tgaagccact gttgtaacta ctgatgacat
      421 tgttcacgag gttaatctgg aaggaaagcg tattggctac gtgattaaga cagaaaataa
      481 agaaacccca ttcactgtgg ttgatatcga cggtccatca ggcaacgtta aaacacttaa
      541 cgatggtgta aaaaaaatgt gtctggtgca cataggaaag aatctgcccg cagaaaaaaa
      601 agccgaattt ctggcaactc tgattgcaat gaaattaaaa ggtgaaatct gaaaaaaaga
      661 aagcctgcac actgtgcagg cctgagtgaa gaacctggga catttattca tcactcgcag
      721 taattttaat ctgagttgag gttaaaaaac aatgaacacc gataaacaag tttacccact
      781 gtattacgaa gcaaaaaatg acaaagtaag aaaacgtctc ggtattaaag gcggttttta
      841 ctgggctgaa gcgaaaaaat tatccattgc catctcccgt ggtgctgttg cgattgacga
      901 tgcaggctac gatgaagatg actttaaaaa acctgttcgc gtcaatttgc ccgttgttga
      961 tgacctcccg ccagaaggcg tatttgatac ggaattctgc aaccgttacg aaaaaggcgg
     1021 ggaagatggc atcacaatgg tatttatcgc atcttctccc tctgttcagg acaaaccggc
     1081 cagcactgac aataccaacg tcaacggcga agacatgaca gaaattgagg agaacatgct
     1141 cctgccagtt tccggtcagg aactgcccat tcgctggctt gcgcaacacg gcagcgaaaa
     1201 accggtaacg cacgtttcac gcgacgaact ccaggcatta cacattgctc gggatgaaga
     1261 actaccagct gttactgccc tagctgtttc ccacaaaacc agcctgctcg atccgctgga
     1321 aattcgcgat ctccacaaac tggtgcgtga tactgacaga gttttcccta atccaggcaa
     1381 ttcaagcctg gggctgatga ctgctttttt cgaagcatac atggacgcag actacaccga
     1441 tcgcggtctg ctgacaaaag agtggatgaa aggaaatcgt gtttcacgca tcacgcgcac
     1501 ggcttccggc gctaatgctg gcggcgggaa cctcaccgat cgcggcgaag gtttcgttca
     1561 cgatctgacg tcactggcgc gcgacgtagc cactggcgta ctggctcgtt caatggacgt
     1621 ggacatttat aaccttcatc cggcacacgc taaacgtgtc gaggaaatta tcgctgaaaa
     1681 taaaccgccc ttttctgttt tccgcgacaa attcatcacc atgcctggcg ggatggatta
     1741 ttcccgcgcc atcgtggttg cgtcagtgaa agaagcaccg attggtatcg aggtcatccc
     1801 cgctcacgtc actgaatatc tgaacaaggt actgactgaa accgatcatg ccaaccctga
     1861 tccggaaatc gtggatattg cctgcggtcg ctcctctgcc ccgatgccgc aacgtgtaac
     1921 agaagaagga aaacaggatg atgaagaaaa aacgcagcca tctggcgcaa tggcagatga
     1981 acaggcaacg gctgaaacaa tggaaccg
//
LOCUS       DADMGC010000274         1983 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.277, whole genome shotgun sequence.
ACCESSION   DADMGC010000274 DADMGC010000000
VERSION     DADMGC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.277"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..1334
                     /locus_tag="JNZ73_004242"
     CDS             <1..1334
                     /locus_tag="JNZ73_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAY8734267.1"
                     /translation="VFSITKKHQPYEFKNFISAWLDTEHIDRGLLTKEWRKGNRVSRI
                     TRTTSGANAGGGNLTDRGEGFVHDLTSLTRDVATGVLARSMDVDIYNLHPAHAKRIEE
                     IIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLTE
                     TDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQPSGAMTDEQATAETVEPNA
                     TEHHQNTQPLDAQSQVNSVDEKYQELRAELHEARKNIPPKNPVDADKLLAASRGEFVE
                     GISDPNDSKWIPGHHISSNEVKKTENEVRQTEDKQHQNSEPEEGTPCNQTGEDNCPDC
                     GAVMGDATYQETFDEKKPDEAQEEEPKKTEKADDLLPENAGSDQHNDSNNETGEDELN
                     WKKQVLIAAVYGLCANPACIAYAPAIPDIAMMIANKLENFGVTSDERLPDF"
     gene            1312..1608
                     /locus_tag="JNZ73_004243"
     CDS             1312..1608
                     /locus_tag="JNZ73_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HAY8734268.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            1668..>1983
                     /locus_tag="JNZ73_004244"
     CDS             1668..>1983
                     /locus_tag="JNZ73_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY8734269.1"
                     /translation="MGFAGDGKTYTASELAIGLVMLMRQRGIEAGNRPVMFLDTETGS
                     DWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMIIDSISHFWTGLCDEYARR
                     RNR"
BASE COUNT          596 a          475 c          493 g          419 t
ORIGIN      
        1 cagttttctc tatcacgaaa aaacatcagc cctatgaatt taagaatttc atttcagcct
       61 ggctggatac cgaacacatt gatcgcggtc ttctgacaaa agaatggcga aaagggaatc
      121 gtgtttcacg catcactcgc acgacttccg gtgctaatgc tggcggcggg aacctcaccg
      181 atcgcggcga aggtttcgtc cacgatctga cgtcactgac gcgcgatgta gccactggcg
      241 tactggcccg ttcaatggac gtggacatct ataaccttca tccggcacac gctaaacgca
      301 ttgaggaaat tatcgctgaa aataaaccac ccttttctgt tttccgcgac aaattcatca
      361 ccatgcctgg cgggctggat tattcccgcg ccatcgtggt tgcgtccgtg aaagaagcac
      421 caattgggat cgaggtcatc cccgcacacg tcactgaata tctgaacaaa gtactgactg
      481 aaaccgatca tgccaaccct gatccggaaa tcgtggatat tgcctgcggt cgctcctctg
      541 ccccgatgcc gcagcgagta acagaagaag gaaaacagga cgatgaagaa aaaccacaac
      601 catctggcgc aatgacagat gaacaggcaa cggctgaaac agtggaaccg aatgcaactg
      661 aacatcatca gaacacgcag ccgctggatg ctcagtcaca ggtaaattct gttgatgaga
      721 aatatcagga actgcgggca gaactccatg aagcccggaa aaacattccg cccaaaaatc
      781 ctgtcgatgc agacaaatta ctggctgcct ctcgcggaga atttgttgaa gggattagcg
      841 acccgaatga ttcgaaatgg attcccggtc atcatatctc ctcaaatgaa gtcaaaaaaa
      901 cggaaaatga agtgcgacag actgaagata aacagcacca gaacagcgaa ccggaggagg
      961 gaacaccctg caatcagact ggcgaggata actgccctga ctgtggcgcg gtgatgggag
     1021 acgcaactta tcaggaaact tttgacgaaa aaaaaccaga tgaagcacag gaagaagagc
     1081 cgaagaaaac ggaaaaagct gatgatttgt taccggagaa cgctggcagc gatcagcata
     1141 atgatagcaa taatgaaact ggcgaagatg aactcaactg gaaaaaacag gtgttgattg
     1201 cggcggttta cggtttatgt gcgaatcctg cgtgcatagc ctatgcgcct gcaatccctg
     1261 acatcgcaat gatgattgcc aacaagcttg aaaattttgg agtgaccagt gatgagcgct
     1321 tacccgattt ttgatcgtat tgaagaaatg gcatgggctc gccactacca gcagatcgtt
     1381 cgtgaagaaa aagaaacgga actggcggac gacctggaaa aaggtctgcc tcagcacctg
     1441 tttgaatcgc tctgcatcga ccatttgcaa cgccacggtg ccaataaaca ggcaatcagc
     1501 cacgcatttg atgatgatgt tgagtttcag gagcgcatgg cagaacacat ccggtacatg
     1561 gctgaaacca ttgcccacca ccaggttgat attgatttgg aggactaaaa tctatggcgc
     1621 tttttcaacg agcaataaac acacaggcct atctaaaagc aggaattatg gggtttgctg
     1681 gcgacggcaa aacctacaca gcaagcgaac tggctattgg tctggtaatg cttatgcggc
     1741 aacggggaat tgaagctggt aaccgcccgg taatgtttct ggacacagaa acaggctctg
     1801 actgggttaa accccgcttt gatgcggaaa atatcgaatt atttacggca aaaacccgct
     1861 cgtttgttga tctgcttgaa gccattaatg aagcggaaag cagcggttcc gtaatgatca
     1921 tcgactccat cagtcacttc tggaccggat tatgcgacga gtatgccaga cgccgaaacc
     1981 gta
//
LOCUS       DADMGC010000275         1972 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.278, whole genome shotgun sequence.
ACCESSION   DADMGC010000275 DADMGC010000000
VERSION     DADMGC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.278"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JNZ73_004245"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JNZ73_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HAY8734270.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
     gene            1494..>1972
                     /locus_tag="JNZ73_004246"
     CDS             1494..>1972
                     /locus_tag="JNZ73_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HAY8734271.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLH"
BASE COUNT          563 a          340 c          475 g          594 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg
     1261 tcagggatta aaagatgaac gtaaatttat tcaacgcatt aattttaaag ggttttattg
     1321 tttgttgacg aaaacaggaa tcatgttcgg tctcttttta tctgttaaaa gccagaagca
     1381 tttccttcgc tgactttata gtcaaccata acacacactc tactgtctga gtccagcgtt
     1441 ttttaacatt cttgttaaga ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac
     1501 agcctgcgcc cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg
     1561 gcgatataca cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc
     1621 cgtggcgaga tttacttatc tcagtgggag acgttatcga tcgtgggccg caaagtttac
     1681 gttgtctgca gttactggaa caacattggg ttcgtgcggt aagaggcaat catgaacaga
     1741 tggcgatgga tgcgctggca ttccagcaga tgtctttgtg gttgatgaat ggcggcgact
     1801 ggtttattgc gctggcagat aatcaacaga aacaagcgaa aacggcgctg gaaaaatgtc
     1861 agcatttgcc ctttattctt gaagtacaca gtcgcaccgg caagcatgtt attgctcatg
     1921 ccgattatcc agatgatgtt tatgaatggc aaaaggacgt tgatttgcat ca
//
LOCUS       DADMGC010000276         1965 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.279, whole genome shotgun sequence.
ACCESSION   DADMGC010000276 DADMGC010000000
VERSION     DADMGC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.279"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(1..1178)
                     /locus_tag="JNZ73_004247"
                     /pseudo
     CDS             complement(1..1178)
                     /locus_tag="JNZ73_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544745.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1388..1831)
                     /locus_tag="JNZ73_004248"
     CDS             complement(1388..1831)
                     /locus_tag="JNZ73_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734272.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLPRHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTVLPGHAAIPLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFFLTR"
BASE COUNT          460 a          514 c          499 g          492 t
ORIGIN      
        1 cacaggtctg tcgccccttc agttgccagt tcgctgtctt tacagacgta atcttttttc
       61 atgcgaccga aggcttccct gacaaaatca tcgacctagg cttcatcaac aggaagggtt
      121 ccgtttttcc gtaactcatg aagcgcacgg ttagccaggt atgcctggcg tgaggatccg
      181 ggataaacgc gggaggcggg cagcacatcg ctgttcagca ttgccggtac cttctgacag
      241 actgcatcag ccccatcgcc attacggtct ttttcatcaa atttattgac cagcacaaac
      301 aggcggccga ctgacacatc agcaatggca ttaagctctt tacgaacctc ctcatcggct
      361 ttcgagttca tctgggtgta gtccatcacc gccagcaccg cagaggcttt ctgcagttga
      421 tcccgcatca tcacttccat ctgcggttgt ccggcttcat tagggccggg ggtatccacc
      481 agtgtgagtg ttccctggca ttcatccatc cccaccagat ggctgaattc cacgtcgatc
      541 accggcagtt tctgcacttc tgcgtattca tcaaaaggaa attcactccc cattgccgca
      601 gcaagacgaa ccagatcgtt taacgatgcc agtccggtaa agattccctc ctccccgtgg
      661 tattcatttt tgagccagcc atcatccgtc agaatatcca gcagttcgcg ggtatccccg
      721 gtttgctgca gggtctgtgc gacctgctgt cctgccgggg tggcgagttt ttcctgcagt
      781 gtgattaata aattgcggac aggctgaata tgttccagat ggagaaccgg ctcagttttt
      841 ccgggaacgt ggcggatgag cgtcggtaca gaggtcatgg ggcggttacg gttaggcaga
      901 atttcctgcc ccacaatggc gttgatggtg gttgatttgc ctgctttcat cgtgccgaca
      961 acagccagca ccatctccct gcgggcggtt ttgatttgtt cgccctccag ctccttaatt
     1021 cttttcagtg ctttctgttt atcaaaaagc tgcccgtttt cgttcttacc gtcagataac
     1081 acatccggct catcaaccat ttgccggagc agattgatgc tcagttgcaa aagccggtcg
     1141 gcttcatcac aaagcagggt gatgttcttt tcgtgcatta tttaaccttc ctgagacgca
     1201 ttttcatgac aaccacggag gaggccattt actacaagcg atcgcttata acgatcaata
     1261 taatagaaat gatcgtcata ttctattaat atgaaaaaca caacgcaagg gggacaaccc
     1321 gggtatggct gcgtttgcat tgtgttttca tagagttaaa aatctgtaac gacaacccgg
     1381 cacatcacta ccgggttaag aagaaaatcc agttccatac cgcgcgggcc acagagacca
     1441 cggtgtcacg aacggcgcgc agggccgtac ggacaacaga ggcaacacag acactctccg
     1501 ccgtgtcaaa tatccggtcc accgcaccgg taaactgttc acgggtctga gtgcggacag
     1561 actccgtgcg cagttcgtcc tgcaggcgtg tcatcagagg gatggcggca tgtccgggaa
     1621 gcactgtcat gagtgcactg accggggtga cctccccctg aaacagtgca ttacagagtg
     1681 aggatttacc ggccccgctt ttacccataa tgccaggcac gggttcgtga cgggggagtt
     1741 tgcgcagatg ttccaggatg tgacgggaaa tcgggtaagg cagggaggag agcggttttt
     1801 caattgcctc aatggcatca gacggattca tacatgtatt cccagtgaaa aaagacaaaa
     1861 ccccgtgcac cggaaaggtg gcgggggatt cacggagata atatctttct gaaattattt
     1921 tgaatgcagg tatatgtggc tgatttgtat cagctaatga gtaca
//
LOCUS       DADMGC010000277         1955 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.280, whole genome shotgun sequence.
ACCESSION   DADMGC010000277 DADMGC010000000
VERSION     DADMGC010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.280"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..269)
                     /locus_tag="JNZ73_004249"
     CDS             complement(<1..269)
                     /locus_tag="JNZ73_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734273.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRH"
     gene            complement(271..549)
                     /locus_tag="JNZ73_004250"
     CDS             complement(271..549)
                     /locus_tag="JNZ73_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734274.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDSCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            490..675
                     /locus_tag="JNZ73_004251"
     CDS             490..675
                     /locus_tag="JNZ73_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734275.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(717..929)
                     /locus_tag="JNZ73_004252"
     CDS             complement(717..929)
                     /locus_tag="JNZ73_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY8734276.1"
                     /translation="MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            complement(1132..1350)
                     /locus_tag="JNZ73_004253"
     CDS             complement(1132..1350)
                     /locus_tag="JNZ73_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734277.1"
                     /translation="MFKCYCTLIVGGIMKNALQFLFVAFWLFASCMPIIFTARYMEKI
                     DVLILMFGHINALFLGVFMAVMCIEYWR"
     gene            complement(1524..1859)
                     /locus_tag="JNZ73_004254"
     CDS             complement(1524..1859)
                     /locus_tag="JNZ73_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734278.1"
                     /translation="MARVEGELFKIARASLEAEPIAWRYRYVKKGVMDSQGELWVGDW
                     KYVPKKEDCNDRPNYEIQALFTAPPVPVTSEELVKAVHFYEQLKRENPPASGNQINGL
                     TMSVKRPAN"
BASE COUNT          484 a          506 c          435 g          530 t
ORIGIN      
        1 atatggcgca acagccagtc agacaatgcg ccagcaccac cagcagcacg aatcacccgt
       61 gcgttactga aaaacggcag caatgttttg tcttccacta gcggctggcg aacggcagga
      121 acgaccccgg acggcagatt acgcatgctt ttcggttccg gctccaccag tacccgggta
      181 ttgtggaata ccggcatgga ttcacggcct ggcttaagga ccaccagccc gagttccgga
      241 accagaacag gtcgaagtaa tacccgcaca ttacctccag atccgttgct ggaatgtgca
      301 ggacggacgc ggtggccgtt cggaataagg gagcctgacg aagattatcc agtgacgata
      361 atcgagactg agggctttct taatctcgta tccgtgtctg cggtagcgct gaatcagcca
      421 ttcagcctgt tcttcggtgc aggagtcgtg ctgataccag tcagatttga atgcatgaga
      481 acgccgcccg tgcctgctgg caggggcggc agagttatcc gaattgtaaa atttggtatc
      541 gtgcgccatc tgttttctct gctggcgcag caggtgccag ttgttcaggc tgacggatgg
      601 attgtaaacc agaacgacca gaaaaaacaa aacccgccga agcgggttaa gtgcgggtgc
      661 gttgaggatg cctgacacat cagcggtggc gagggatttc tcccccgccg ggtctcttac
      721 tcctcaggtt cgtaagctgt gaagacagcg acctccgtct ggccggttcg gattcgtacc
      781 tcgcagaggt ctttcctcgt taccagtgcc gtcactatga cggttaaaca gatgacgata
      841 agggcgatta acatcgcctt ttgctgcttc atagcctgct tctccttgcc tttcggcacg
      901 taagaggcta acctacatgt gttcagcatg gattgagcct cagattaatg ttaagcgtct
      961 tgcaggacgc gtaatgttaa ctggggcttt tctctatctg ccttttggtg ttcatgcctg
     1021 agacagatag cctcaagcac ccgcagtcat tctacttaac taagatttcc ctgcaaaccg
     1081 tttttgtccg gcacagtaaa tatccaacta aaccaatagc gttcgctgta tttaccgcca
     1141 gtattcaatg cacatgaccg ccatgaacac ccctaaaaaa agggcattta tatgtccaaa
     1201 cattaatatc aaaacatcaa ttttttccat ataccttgct gtgaagatga tgggcataca
     1261 tgatgcgaac aaccagaacg caacaaacaa aaactgcaat gcgtttttca ttattccccc
     1321 tacaatcaat gtgcaataac atttaaacac acctcaattt ggccggacat ataaatatct
     1381 aaaccagaaa aaatcactta catagcgtta caaactcttt agtctaaata ctcatcgtaa
     1441 aacattcccc atacttatca gtccgttccg cgccaggtag cttattgcct tatctggcaa
     1501 cctataatca ggtttccgct ttttcagttg gctggtcgtt taaccgacat agttaaccca
     1561 ttaatctggt tgccggatgc tggtggattt tcgcgtttta gttgttcata aaagtgcaca
     1621 gctttaacca gttcttctga tgtaaccggg actggtgggg cagtgaataa ggcctgaatt
     1681 tcatagttcg gcctgtcgtt acaatcctct tttttcggta catatttcca gtcaccaacc
     1741 cacaactccc cctgagagtc cataacacct tttttcacgt agcgatatcg ccacgctatc
     1801 ggctctgctt ccagcgatgc ccgtgcaatt ttgaataact cgccctctac tcgcgccatc
     1861 cctgaattgg ggtggcattt cgtaatcgct atttttaatt tggcttcttc gattaattgt
     1921 tcttttgtta attcagtcat tttcattacc gccct
//
LOCUS       DADMGC010000278         1946 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.281, whole genome shotgun sequence.
ACCESSION   DADMGC010000278 DADMGC010000000
VERSION     DADMGC010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.281"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            134..625
                     /locus_tag="JNZ73_004255"
     CDS             134..625
                     /locus_tag="JNZ73_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734279.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAERIAKELASQKE
                     RTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            709..1698
                     /locus_tag="JNZ73_004256"
     CDS             709..1698
                     /locus_tag="JNZ73_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734280.1"
                     /translation="MNMTKNAFQFGIEPVHITDNDNLQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGAEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
BASE COUNT          481 a          513 c          482 g          470 t
ORIGIN      
        1 cgcttaaccg ttagtagctc acctactcaa tacctaattt aaacaccttt tgagatgacc
       61 gatgcgggga gacccgtagg ccttactgtg cctgcgatac cgatagtctc aactctcaag
      121 aggagaatta cctatgatgt tcaacaacaa caactggaag ctgtcagtca ccgacatcaa
      181 cctttatgaa aacactgtga gtctggacgg tcagtcctat ccgttgtcgc tcgctatcaa
      241 gaccctcatt ccgggctacc tgtcaggctt accgtccacc agccgcgagg cgatggagtt
      301 actggaagct ctggctgaag ctggcgtgac cattggtaac ttcttcagta atgacctgat
      361 gaccgcctac caacgccgcc agatgaacaa acgagctgaa gctgagcgta tcgctaaaga
      421 gctagcgtct cagaaagagc gtacccgcga aatgttcatg accgaagacg aatggcaaaa
      481 agagctacag cgccgcgaac aggtcaaagc tgaacgccgg acctatggtg aaaacctccg
      541 cagcgctact cattccgctg gtcgctcacg tgccgctatc gtcgctgacc ttgagtcagg
      601 cggtaactgg atggacagct tgtaagcaag cgtccctcat ctgagaacac acggtccctg
      661 actgcctgtt agggacttca agtatttgac tcaaggagat taattataat gaatatgact
      721 aaaaacgctt tccagtttgg cattgagcct gttcatatca ccgacaacga caaccttcag
      781 gttaatgagg gccttccgac taacgccgac cctcaggtct atgccctcca gttagctaag
      841 actgttaagg ctatgctgaa cggtgtactt aaagacgctc aagataacat cccgttccct
      901 gtagaagtcc tgccgacccg taacagctta ccgactccaa tcattgctca cactctggct
      961 gaccgctctg tgctggtccc tgtccgtggt ggtaagcgac ctgaagttgt gaccgctccg
     1021 tctggcgctg aaatcaccgt tgagccgatt gaacaggcga tcctcgtgtc tcaccaaaca
     1081 aagctttggg accagaagtc gaccactggt ttcactcagg ggacgctcca gcaggacgca
     1141 ctgaacatct gcgataacgt gattcgtacc attaactcta agatggttga cgtgctggag
     1201 tcctcaaagc tgctgaagac cgtagaactt ccggccctga ctggtagcct gaccgctaag
     1261 gctgacgcta tcatggatgc cctgtacgag aacacggagt cttctttcgg ttctgaggtt
     1321 tctgactacg gcattatcgc ccatgagtca cacctcaagg ctctctcccg tctggctgct
     1381 aagcagggct tcggtggtga agacgctatc gtcgatatgc tggggactga cgtcgcctat
     1441 tacaacggtg aagaccgtgg cgtgttcatg atggctaagc gttttacggc tctgagcttc
     1501 ggttgcttcc gtcacgatgg cgagagcatt actgtggtcc tttcacgtga tggagactcc
     1561 cagtctcacg accttgagat tctcggcaag gtgtttgttg tagctgaagc tgctacgacc
     1621 atcaagatgg gtactggctc ggctactgct gtcctgcctg tagtcaaacg cctgagcttc
     1681 actaaagaag ctaactaaca ataacgacta ttggtcagac ttccgaactg gatgtctggc
     1741 ctttattttg tactagtaat ctagttcgtg aatacagaaa gtaggagggc caaatggacc
     1801 tacacaaagt ctatctcgtg gtcttcctgc taaagctggg actggtggtc tggctatggg
     1861 ctattagacg atgagttttg actgaaaaat gtgagacctc atctaatata aagagcgagc
     1921 caaaagcccc catagcctga cctgag
//
LOCUS       DADMGC010000279         1941 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.282, whole genome shotgun sequence.
ACCESSION   DADMGC010000279 DADMGC010000000
VERSION     DADMGC010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.282"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(1113..1625)
                     /gene="clbS"
                     /locus_tag="JNZ73_004257"
     CDS             complement(1113..1625)
                     /gene="clbS"
                     /locus_tag="JNZ73_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin self-protection protein ClbS"
                     /protein_id="HAY8734281.1"
                     /translation="MAVPSSKEELIKAINSNFSLLNKKLESITPQLAFEPLLEGHAKG
                     TTISVANLVSYLIGWGELVLHWHDQEAKGKTIIFPEEGFKWNELGRLAQKFYRDYEDI
                     TEYEVLLARLKENKQQLVALIERFSNDKLYGKPWYNKWTRGRMIQFNTASPYKNASGR
                     LNKLQKCLAE"
     gene            complement(1660..>1941)
                     /gene="clbQ"
                     /locus_tag="JNZ73_004258"
     CDS             complement(1660..>1941)
                     /gene="clbQ"
                     /locus_tag="JNZ73_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis thioesterase ClbQ"
                     /protein_id="HAY8734282.1"
                     /translation="MTIFTPIIKNDYRLYEQYVFQAKARTLTCPIVLFHGDADNLVMQ
                     DELLAWEKFTTRKTRTIIFPAADHFFVDKHFEQVVGYVNQTIESLEIVG"
BASE COUNT          556 a          448 c          386 g          551 t
ORIGIN      
        1 gatgtccgtc tatatcagat gggcgtctaa gttaccaatt ctcgtctgat ggctacatac
       61 ggcggtcagt ttacgcttac tacaatgatg ataatgcgat tatataaagg tcaaaccata
      121 tggttgttag cggatttcaa caacggtgct gtactcgctg agcggcacat gaagtttgaa
      181 taatgtggaa acaacaaatg gctacattaa gtcattggac atatacagag atggttggca
      241 aatcgagtac acgatatgtt gcctgaaaag tcgaccgaac agaactataa atatcaagtc
      301 ggcataacac atctaaattt cataagggaa gatgtgccac cggggacgtt tttttcacca
      361 cggggacttc gggtataacg atgcaatccg aagaaagaaa tccaatgttg gcgatattca
      421 tcatcgcttt attcgtccac tcatgcgaat ggcaataaag cgactcacca aaacagtttc
      481 tttagcttcc ctctaccaaa attccctatg atcgcatgtt agaactctgc gtgctgaact
      541 tcgggccata tggacggtat cattcatgca cttgctcctt tgaaattgct ggatatcatt
      601 cgtctttatc tgcaccggcc ggcacaaata gctgtagggg aacccggcgc aggcaaaaag
      661 agcgacttta acgcgttttg caggactccc cgttgccttg cagggagaca ctgtatcatc
      721 aaacaccatc ttaatcccag tacattaccc tccatcctgg ctcgtaccaa atcagaggaa
      781 aggcctcgcg gcaatagatt ttcccggcga agctcggagt gaaatacagt gcagcaatat
      841 ccgacatcag aatttgatcc tcctgcgtca ggatacgaaa aaacatcccg atttgagcac
      901 ccttgtcgaa aaaaaatcac cttattatga agattggttc ttaatacaca tgcgttcgca
      961 tgtgcttatg ttaagtagac ttattgtctt acacatttta tccgtatcat taaccagaat
     1021 atgttccgga gcagcgggtg atagtgccgc cagtccacac tcatcgctgc ccggaaaaat
     1081 catcagtggg gaggcaaacg gtaagcaccc cgctattctg caagacattt ctgcagttta
     1141 tttaacctcc ccgaagcatt tttataaggc gaggcggtat taaattgaat catacgacct
     1201 cgggtccatt tattatacca aggtttaccg taaagcttgt cgttactgaa tcgttcaatc
     1261 aaagccacga gttgctgctt attttccttt aaccgtgcca ataaaacttc gtactctgta
     1321 atatcctcat agtcacggta gaatttctgt gctaaacgcc ccaattcatt ccatttaaat
     1381 ccttcctcag gaaaaataat agtttttcct tttgcctctt ggtcatgcca gtgtaacacc
     1441 agctctcccc agccaatcag ataggaaacc agattcgcta cgctaatcgt agtccccttc
     1501 gcgtgccctt ccaatagagg ttcaaaggcg agttggggcg taatagattc tagcttctta
     1561 tttaataaag aaaaattact attaatagct ttaattaact cttcttttga tgatggaaca
     1621 gccatatcta ttgctccttg tatagttaca caactatttc taccctacta tttcgagtga
     1681 ttcaatcgtc tggttcacat aacctaccac ctgttcaaaa tgcttatcga caaaaaaatg
     1741 atcggcagca ggaaatataa tagtccgcgt ctttcgtgtg gtgaattttt cccatgcaag
     1801 taattcatcc tgcattacca gattgtcagc atcgccatga aatagcacga tcggacaggt
     1861 taatgtgcgc gccttggcct gaaatacata ctgctcatag agccgataat cgtttttaat
     1921 gatgggggtg aaaattgtca t
//
LOCUS       DADMGC010000280         1932 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.283, whole genome shotgun sequence.
ACCESSION   DADMGC010000280 DADMGC010000000
VERSION     DADMGC010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.283"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..740)
                     /gene="melB"
                     /locus_tag="JNZ73_004259"
     CDS             complement(<1..740)
                     /gene="melB"
                     /locus_tag="JNZ73_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HAY8734283.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVA"
     gene            complement(843..>1932)
                     /gene="melA"
                     /locus_tag="JNZ73_004260"
     CDS             complement(843..>1932)
                     /gene="melA"
                     /locus_tag="JNZ73_004260"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAY8734284.1"
                     /translation="GYEPCTVTDFEVCKRHGLEQTIADTLGPGGIMRALRTIPHLWQI
                     CENMTEVCPDATMLNYVNPMAMNTWAMYARYPYIKQVGLCHSVQGTAEELARDLNIDP
                     ATLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYEAGQAPKPNIHGNTRCQNI
                     VRYEMFKKLGYFVTESSEHFAEYTPWFIKLGREDLIERYKVPLDEYPKRCVEQLANWH
                     KELEEYKNASRIDIKPSREYASTIMNAIWTGEPSVIYGNVRNDGLIDNLPQGCCVEVA
                     CLVDANGIQPTKVGTLPSHLAALMQTNINVQTLLTEAILTENRDRVYHAAMMDPHTAA
                     VLGIDEIYALVDDLIAAHGDWLPGWLHR"
BASE COUNT          511 a          507 c          466 g          448 t
ORIGIN      
        1 ctggctacat tataagcaag agccataccc aagaggcatg aaagctgatc gtttttataa
       61 ataagcgcaa cgatggcttt aagtgtcaga tggcttcctt cagcagacga ttgattgtct
      121 gacgaaaaga cttcatgcac attgcgcaga gtgatgatgg ttgaaacaat aaaaaaggcg
      181 atcaatacca gagtgaacat ctgaaagcca aatccccgat cgccaccgcc gacataatta
      241 acaaatggta gcgtcacacc tgccgtaaca aagcctgcca gactggcaaa aaaacgcgga
      301 taaggaacca gttgttcgcg ttcacgttta tcgagcgtga tggttggaac cagcgaccag
      361 aagggaatat ccataatggt gtaagtcatg ccccagagga tgtaggtcac gcaaacaaag
      421 acaatctgag tagtaccttc aaacagatgc gcactaaaga ggagaaataa gattacagag
      481 tttgccaacg taccgatcag gatccagggt ttgaacttac cccatcgcga tcgcgtagca
      541 tttacaatcc atcccataat cggatcgtta atagcatccc agatcctcgc caccagaaac
      601 aaagtaccga ccaaacccac agacagcccg acgacatcgg tgtagtaata catgaggtac
      661 atatacacaa tgccgatcgc aaaatccttc ccgaacgctc caaatccata actgagtttt
      721 gtagtcattg aaatgctcat agggtatcgg gtcgctgtta ccaacgcccg ccttctgtta
      781 ttggtatcgt cagaggtgtg cgccggaata aatcccccgg cgcagtagcg tttagtcgcg
      841 ttttaacggt gcaaccagcc tggcagccag tcgccgtggg cggcaatcag gtcgtcaaca
      901 agagcatata tttcgtcaat gcccagcacg gcggcagtat gcgggtccat catcgcggcg
      961 tggtaaacac ggtcgcgatt ttccgtaaga atagcttcgg tcagcagcgt ctgtacgttg
     1021 atgttggttt gcatcagggc ggccagatgc gaaggtagcg taccgacttt ggtcggctga
     1081 atgccattag catcaaccag acaggctact tccacgcaac atccttgtgg caggttatca
     1141 atcaaaccat cgttacggac gttgccgtaa atcacactcg gctcgccagt ccagatagcg
     1201 ttcatgattg tgctggcata ttcccgtgac ggtttaatat caatccggga ggcgttttta
     1261 tactcctcca gctctttatg ccagttcgcc agctgctcga cgcagcgttt cgggtactca
     1321 tccagcggta ctttataacg ctcaatcaaa tcctcacgac ctagcttaat aaaccacggc
     1381 gtatattcgg caaaatgttc tgacgattcc gtgacgaagt agcccagctt tttgaacatt
     1441 tcgtagcgca caatattctg gcagcgagta ttaccatgaa tatttggctt cggtgcctgc
     1501 cctgcttcat aagccgccag cagttccggg tagagattca cgtaactgcc gtcggcggtt
     1561 ttgcgctcca gctccaggta aaacgccata tggttgatac ctgcgcaacg gtaacgcagc
     1621 gtagccgggt cgatattgag gtcacgcgcc agctcttccg ccgttccctg caccgaatgg
     1681 cacagcccga cctgtttgat atacggatag cgggcataca tcgcccaggt attcatcgcc
     1741 attgggttaa catagttgag catggtggca tcggggcaga cttccgtcat gttctcgcaa
     1801 atttgccaca gatgcggaat ggtacgtagc gcgcgcataa taccgcccgg ccccaacgta
     1861 tcggcaatgg tttgttccag accatgccgc ttacagacct cgaaatcagt caccgtgcaa
     1921 ggttcataac cg
//
LOCUS       DADMGC010000281         1923 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.284, whole genome shotgun sequence.
ACCESSION   DADMGC010000281 DADMGC010000000
VERSION     DADMGC010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.284"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(133..1587)
                     /gene="ospC2"
                     /locus_tag="JNZ73_004261"
     CDS             complement(133..1587)
                     /gene="ospC2"
                     /locus_tag="JNZ73_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HAY8734285.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFINNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          610 a          331 c          300 g          682 t
ORIGIN      
        1 tccaggattc ccggggcgtt caccacatgc aatgtgttgt tgcaggggaa cggtctgcac
       61 catcccctat atcgatcaat ataacaaaaa tgacaacgtc agattcaggg cacaacctaa
      121 atatatttta ggtcacctcc caaatcgttt gcctgatacc gctccatttt ttaataaaaa
      181 atcaattgtt tttgaatctt tgcttttcat tgcattgtca agcatagtgt ccccactatt
      241 tgctttttga aatcttttgt ttacatcaac caatccatta ttaataaaat attctagcac
      301 tttatagttg gcactatctt cactaagtaa atactcaaca tcatacagct cacaatacat
      361 attgttgttc attttctcca tctctgcgac atcttgtttt gtaaaattaa atttagatag
      421 taaataaagt gccatatcag attttgcatt gcctagagaa taagcaagag cttgatgagc
      481 cattttttta tcagtcacct tattgttaat ttcttcataa ttggatgctt taatggcatc
      541 ctccaaagat atatctctga gtcttatctt tttgaagtta tctgtgctta gcattcttgg
      601 tatatgatac tcaagctgaa acacaaagtt tataattcta tccagactaa caggatcaat
      661 gtttttatta taacaaaact ctcggaatct ttgatcttta ctctttctta taaaatcaat
      721 aagatgcaac ccaattccaa gcctcatatc ttttgtatta aatattggca catcagtttt
      781 tccctgatta ccatgcacat acctgctaac ttcatccaca acctctttaa agttatctaa
      841 gaaaaatgat tggtgctcat ggtaaaaaac aggtggaata gcattatcaa agtggtcggt
      901 taatgtcata tatccatatg gagagtcatg atcaatgata tacgcattag caccaaaatc
      961 cacagtatga tgtgttttat ttagtgggag tgtttcttga tgattggaaa tttcaacccc
     1021 aaaaaaaaca aaatcgtggt tagctatttc ttcaatatcc tcagagaatg tgtttttata
     1081 tgtttgtata ttctcagact taattttatc aaaggactta atattaagag tattatttga
     1141 caggttagcg ttactttgat gtctgaagtt aaaatcagaa ttaataataa tagagagcaa
     1201 gtgacgttca tccgaagaaa gaggatgagt tttttgtatt atttcagaaa gatagtgtct
     1261 ggacacctta tcgaggtgct caagtgaccg aaatgatgac cgtgctccag atggcgcttc
     1321 aatagaaaga tttaatgatt tgaaactggt cccttgagag aacattttac tataactttg
     1381 cgctgctata gacttcctta aaaagctttt cacagtatca gcacagtgat ctaaatgttt
     1441 ttcacttatg ccagaagagg atgagtttgt tacgcatgat atgttttttt gtaatgcttt
     1501 tgtatctttg ttcggattta tttttccatc aacaagatca atattgttct gaacattaat
     1561 atgatttact gcttcaggta ttttcataaa acatccccca ttatgtgcgt ttttatctgc
     1621 agttatttag aataatatta tctaacttaa tattttcttg tatgtattat aacgaacata
     1681 ttttaatatg ttgtacgatt ttcactctga aatcccctga gtttaatcac acttccttta
     1741 ctgaaaggaa ttattgcaac agtattattt gtttttggat atatatcttg cgccttgtta
     1801 tgtagttaat gctcagaata tgatagataa aatagagacg caaaacaggg catgctcata
     1861 acatgatgat aacataaaaa attagtaaca ataaatgaaa gaatcatggg cgctcaacaa
     1921 aaa
//
LOCUS       DADMGC010000282         1918 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.285, whole genome shotgun sequence.
ACCESSION   DADMGC010000282 DADMGC010000000
VERSION     DADMGC010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.285"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(87..1499)
                     /gene="pykF"
                     /locus_tag="JNZ73_004262"
     CDS             complement(87..1499)
                     /gene="pykF"
                     /locus_tag="JNZ73_004262"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HAY8734286.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          481 a          445 c          470 g          522 t
ORIGIN      
        1 attttttatt gcttctggtt atcgattaaa taaaaaaagc gcccatcagg gcgcttcgat
       61 atacaaatta attcacaaaa gcaatattac aggacgtgaa cagatgcggt gttagtagtg
      121 ccgctcggta ccagtgcacc agaaaccata actacgacgt cacctttgtg tgccagaccg
      181 ctctgcagag ccagttcttt acccagacgg tagaaatcat cagtagaagt gatctcttta
      241 accagctgcg gcacaacgcc tttgctcagt accaactgat gagccgtttt ttcgttggtg
      301 gtcagtgcca ggatggtggc atccgggaag tatttacgta ctgcgcgagc agatttaccg
      361 ccctgggtag caaccacgat cagcggagca tccagttttt cagcagtttc aacggcacca
      421 cggcataccg cttcggtgat gcgcagttta cggttgtcat tgttgaactc gagacggctg
      481 ttcatcacgc ggtcggtacg ttcgcagatg gtcgccatga tagaaaccgc ttccagcggg
      541 tatttacctt ttgcggattc accagacagc atcactgcgt cagtaccgtc gaggatggcg
      601 tttgcaacgt caccggcttc tgcgcgagtc gggcgtgggt ttttgatcat ggaatccagc
      661 atctgggtcg cagtgataac gactttacgt gcacggatac atttttcgat catcatcttc
      721 tgggcgaaga taacttcttc taccgggatt tctacaccca ggtcgccacg cgcaaccatg
      781 atgccgtcag aggcttcgag gatttcgtcg aagttgttga ggccttcctg gttttcgatt
      841 ttggagatga tgtggatgtt ttcgccgccg tgcgctttca ggtgctcacg gatttcgata
      901 acgtcagaac gcttacgaat aaaggaagca gcaacaaagt ctacgccttt ttcgcaacca
      961 aagatcaggt cctgtttgtc tttttcagcc agtgctggca gagcaatgga aacgccaggc
     1021 aggttcacac ctttgttttc gcccaggtca ccgttgttca gcactttaca gataactttg
     1081 ttaccttcaa tggcggtaac ttccataccg atcagaccat cgtcaaccag tacggtgttg
     1141 ccaacagaca ggtcagtagt gaaaccttca tacgttaccg caaccatttc gctgttgccg
     1201 ataacagatt tatcagtggt gaaagtaaag gtctgaccag ctttcagaga aacatcgtta
     1261 ccgccttcca gtttcatggt gcggatttcc ggacctttgg tatcaagcag gatagcggcg
     1321 gttttaccag ttttgctcat cacgttgcgc agattctgaa tgcgctgacc gtgttctgca
     1381 tagtcaccat gagagaagtt cagacgcata acgttcatgc cagcgtccag cattttagct
     1441 aacatctctt cagattcggt tttcggtccg atggtgcaaa caattttggt ctttttcatg
     1501 acagtcttag tctttaagtt gagaaggatg ggagaaactt gctttctggg cgcactgccg
     1561 gaaagcgaat ccggtattac gaaagtggtg gtgcctcgct ctaaggatag gtgacatcga
     1621 aaaagcgtgc agaggaatgt gtgcttgtgt ctcagcccaa cgtgagtgcc tgtttttgag
     1681 tcgttgagtt ctacagtcca aagtgctgaa accattcaag agtcaattgg cgcgcattat
     1741 acgctgaaat taatccaaaa ggaaaacaaa aacagcgttt caaaaaatat acaacttgtt
     1801 agacaataaa ttgttatcaa agatttaacg ttccagggaa aaggttacgc aaaaacttcg
     1861 ctgtttgcgc aacgtctggt gccgaagttg tcagtgtgag gataagtgaa agttcatc
//
LOCUS       DADMGC010000283         1889 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.286, whole genome shotgun sequence.
ACCESSION   DADMGC010000283 DADMGC010000000
VERSION     DADMGC010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.286"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(289..1398)
                     /gene="fepE"
                     /locus_tag="JNZ73_004263"
     CDS             complement(289..1398)
                     /gene="fepE"
                     /locus_tag="JNZ73_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAY8734287.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
BASE COUNT          604 a          437 c          321 g          527 t
ORIGIN      
        1 tggcagcggc cactggtaaa caggatgtgc gagagatgta ttgagagttc ttgaagtggc
       61 agccttactg aggctacact agaaggacag atttggtgac atgtctcgca caagactgtt
      121 accacggttc agaagttccc caacttactg aaccttcgaa aaaccacctc cccaggtggt
      181 tttttcgttt tctagagtag aagactacgg ctgtaaggga atgatctcaa gaagatcctt
      241 tgaacttttt ataaaagttg cttataaaat gatgcatcag cacaactttt actcaatcac
      301 actcatcttt ctggcctcca tggcatgacg taaaagtata tagccacatg caagcatccc
      361 tccaatgaca gaagaaagaa ttacaattat ggcattactt ggcccatcct ttctgaccgg
      421 taacgttggg ctcaattgat actgaaatgg cataaaatca acatccccca ctttcgccac
      481 aaccaattga ttcacaacat attgacgatt tcgcaactcc ccactcaatt ccgaaacatc
      541 cttgattgat tttttgatgt tcaatttagt tgctatacca tcagaaccga gagccacagg
      601 aaaatctggg tcatccttca taatctgacc attgctgtat acaggttttt ttattccagc
      661 cgcattagca acctccagtg aataattgag gcgtcgaata tctgcattca gctggtttct
      721 aatttttatc tcatccagtg caagaatttc tttttcaaca ttagttttaa cttctagttt
      781 atttcttata tcttccatca aatcccttac aacaagggaa gaaacataat taatataccc
      841 agacaacaca tcgtgagctt ctttacttgt tggagcagta aaactcagag tccatgatac
      901 atacaatgat gacttgtcgt ttttcttact ggcattatca tcaatcgact tcatattttt
      961 actcaactca acaattgcac gatgcagttc gagagcgcta atgttattct ttttaataat
     1021 ctccataaca taaggagatg atctcatata ctcattcaac gaattaacgg actgaaattt
     1081 cttaataaac agagcaaatg cgctatcacg attgatatca aaatccactc ccagaacatg
     1141 aagcttggaa agctccttat ttaattcact ccattgaaca gactcagcag gagtaaccac
     1201 agcagaacta gtccattttt gtggcaaaac aaaagaaaca agaaagccaa tacaagcaaa
     1261 aaatagaatc accccaacaa tttttatttt tgctgcccat aaaacgctaa tcaagccaaa
     1321 aaggtcaatt tcttcattac cattcggcaa catgttgtaa ttagaaaaat gttcgtcaga
     1381 gacctttgag attttcattg aaaaaaacac aatatattaa catatggttt ttttattcta
     1441 tcatatgtta ggaaaaaacc taaaaaccat tcagcagata tttaacataa tatcttattg
     1501 cgcgcaccgc taaaacgcgc tgaacgcgat tctgccgcaa acaccgcaaa aagagatgtt
     1561 ttccccgggg taaaccggaa aaatgcgtca ggatcgcttt cagctcactg catagctatg
     1621 catgaaagtg aatgacgatc ggtttgggcg catacagtgc ccatgagaca cattttcgga
     1681 cattttcagt tcgtgaatcc ctgactcatt gctacatatc tgaacagaca cccctagcag
     1741 cgcccccagg ggcgtcctgt aaaaaaacac accgcgccgc tagcagcccc ccccctaaaa
     1801 agcggtgttg gcgcagctac aacaccgcgc cgacaccgct tttttaaata tcacaaagag
     1861 agcaagaaga actaatttgt cataactct
//
LOCUS       DADMGC010000284         1888 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.287, whole genome shotgun sequence.
ACCESSION   DADMGC010000284 DADMGC010000000
VERSION     DADMGC010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.287"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            27..1888
                     /gene="cyoB"
                     /locus_tag="JNZ73_004264"
                     /pseudo
     CDS             27..1888
                     /gene="cyoB"
                     /locus_tag="JNZ73_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
BASE COUNT          360 a          492 c          510 g          526 t
ORIGIN      
        1 attaaagggg ttgaggaaga ataaagatgt tcggaaaatt atcacttgat gcagtcccgt
       61 tccatgaacc tatcgtcatg gttacgatcg ctggcattat tttgggaggt ctggcgctcg
      121 ttggcctgat cacttacttc ggtaagtgga cctacctgtg gaaagagtgg ctgacctccg
      181 tcgaccataa acgcctcggt atcatgtata tcatcgtggc gattgtgatg ttgctgcgtg
      241 gttttgctga cgccattatg atgcgtagcc agcaggctct tgcctcggcg ggtgaagcgg
      301 gcttcctgcc acctcaccac tacgatcaga tcttcaccgc gcacggcgtg attatgatct
      361 tcttcgtggc gatgcctttc gttatcggtc tgatgaacct ggtggttccg ctgcagatcg
      421 gcgcgcgtga cgttgcgttc ccgttcctca acaacttaag cttctggttt actgttgttg
      481 gcgtgattct ggttaacgtt tctctcggcg tgggcgaatt tgcgcagacc ggctggctgg
      541 cctatccacc gctatcggga atagagtaca gtccgggagt cggtgttgat tactggatat
      601 ggagtctcca gctatccggt ataggtacga cgcttaccgg tatcaacttc ttcgttacca
      661 ttctgaagat gcgcgcaccg ggcatgacca tgttcaagat gccagtattt acctaggcat
      721 cactgtgcac gaacgtactg attattgctt ccttcccaat tctgacggtt accgtcgcgt
      781 tgttgaccct ggatcgctat ctgggcaccc atttctttac caacgatatg ggtggcaaca
      841 tgatgatgta catcaacctg atttgggcct ggggccaccc ggaagtttac atcctgatcc
      901 tgcctgtttt cggtgtgttc tccgaaattg cggcaacctt ctcgcgtaaa cgtctgtttg
      961 gttatacctc gctggtatgg gcaaccgtct gcatcaccgt gctgtcgttc atcgtttggc
     1021 tgcaccactt ctttacgatg ggggcgggcg cgaacgtaaa cgccttcttt ggtatcacca
     1081 caatgattat cgccatcccg accggggtga agatcttcaa ctggctgttc accatgtatc
     1141 agggccgcat cgtgttccat tctgcgatgc tgtggactat cggttttatc gtcaccttct
     1201 cggtgggcgg tatgactggc gtgctgctgg cagtaccggg cgcggacttc gttctgcata
     1261 acagcctgtt cctgattgcg cacttccata acgtgatcat cggcggcgtg gtcttcggct
     1321 gcttcgcagg gatgacctac tggtggccta aagcgttcgg tttcaaactg aacgaaacct
     1381 ggggtaaacg cgcgttctgg ttttggatca tcggcttctt cgttgccttt atgccgctgt
     1441 atgcgctggg cttcatgggc atgacccgtc gtttgagcca gcagattgac ccgcagttcc
     1501 acaccatgct gatgattgca gccagcggtg cagtactgat tgcgctgggt attctctgcc
     1561 tcgttattca gatgtacgtt tctattcgcg accgcgaccg cgaccagaac cgtgacctga
     1621 ctggcgaccc gtggggtggc cgtacgctgg agtgggcaac ctcttccccg cctccgttct
     1681 ataactttgc cgtagtgccg cacgttcacg aacgtgatgc attctgggaa atgaaagaga
     1741 aaggcgaagc gtataaaaag cctgaccact atgaagaaat tcatatgccg aaaaacagcg
     1801 gtgcaggtat cgtcattgca gctttctcca ccatcttcgg tttcgccatg atctggcata
     1861 tctggtggct ggcgattggt aagcgccc
//
LOCUS       DADMGC010000285         1879 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.288, whole genome shotgun sequence.
ACCESSION   DADMGC010000285 DADMGC010000000
VERSION     DADMGC010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.288"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..1480
                     /locus_tag="JNZ73_004265"
     CDS             <1..1480
                     /locus_tag="JNZ73_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY8734288.1"
                     /translation="FAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIVEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            1488..>1879
                     /locus_tag="JNZ73_004266"
     CDS             1488..>1879
                     /locus_tag="JNZ73_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734289.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTG"
BASE COUNT          521 a          367 c          458 g          533 t
ORIGIN      
        1 gtttgcgttc attattatcg tgttgctggt ggtatatttc atgaccactc gtgtgttccg
       61 tcggcaattt tctgaaatga ccgagctggt taatacgctg gcgtttttgc ccgactcaac
      121 ggatcagatc gaggctctga aaattcgcga aggcgatgcg aaagagatta tcagcatcaa
      181 aaattcgatc gtggaaatga aagatgccga aattgaacgg tcaaataaat tgctctcact
      241 gatctcttac gatcaggaaa gcggttttat taaaaatatg gcgattattg agtccaacaa
      301 taatcagtat ctggctgtgg ggatcatcaa actgtgtggt ctggaagccg tggaagcggt
      361 gtttggtgtt gatgaacgca ataaaatcgt caggaaattg tgtcagcgaa ttgccgagaa
      421 atatgcgcaa tgctgcgata tcgtgacatt caatgccgat ctctatttac ttctgtgtcg
      481 ggaaaatgta cagacattta cccgtaaaat agcgatggta aacgattttg acagcagctt
      541 tggctaccgc aatctgcgca tccataagtc tgccatttgt gaacctttgc agggggaaaa
      601 cgcctggagt tacgcagaaa aactgaaact ggcgatttcc agtatccgtg accatatgtt
      661 ctccgagttt attttctgtg atgacgcgaa actcaacgaa atagaagaga atatctggat
      721 tgcgcgtaat attcgccatg caatggaaat tggcgaacta ttcctcgtct atcaaccgat
      781 cgttgatatt aacacccgcg ccattctgag cgcggaggcg ttgtgccgtt gggtttctgc
      841 ggagcggggg atcatttcac cgctgaagtt cattaccatt gctgaagata tcgggtttat
      901 caatgagctg ggttatcaga ttattaaaac ggcaatgggt gaattcagac attttagtca
      961 acgtgcgtcg ctgaaggatg atttcttact gcatattaat gtttcgccct ggcagttaaa
     1021 cgaaccgcac tttcatgagc gttttaccac catcatgaaa gaaaatggcc tgaaggcgaa
     1081 cagcctctgt gttgagatca ctgaaaccgt gatcgagcga attaatgaac atttttatct
     1141 caatattgaa caactgcgta aacaaggggt acggatatcg attgatgact ttggcaccgg
     1201 tttgtcaaac ctgaaacgtt tttatgaaat taatccagac agcataaagg tggactcgca
     1261 atttaccggc gatattttcg gtactgcggg aaaaattgtg cgcattattt tcgatctggc
     1321 acgctataac cggatcccgg tgattgcgga aggcgtagag agcgaagacg ttgcgcgcga
     1381 attaattaaa ttaggatgcg ttcaggctca ggggtatctg taccagaaac ccatgccatt
     1441 ctccgcctgg gataaaagtg gaaaattagt aaaagagtag tttacgtatg tccagaatca
     1501 ataagttcgt acttacagtc agtctgctga tttttatcat gatttcagca gttgcctgcg
     1561 ggatctacac tcaaatggta aaggaacggg tgtatagcct gaaacagtcc gttattgata
     1621 ctgcttttgc ggtggcaaat attgctgaat atcggcgtag cgtggcaatt gatcttatca
     1681 acacgctaaa tcccacggag gaacagctgt tggttggttt gcgcacagct tacgccgact
     1741 cggtttcccc ctcttatttg tacgatgtcg gtccttatct gatttccagt gacgaatgta
     1801 ttcaggtaaa ggagttcgag aaaaattatt gtgcagatat tatgcaggtt gtgaagtatc
     1861 gacatgtcaa aaatacagg
//
LOCUS       DADMGC010000286         1877 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.289, whole genome shotgun sequence.
ACCESSION   DADMGC010000286 DADMGC010000000
VERSION     DADMGC010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.289"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..557)
                     /locus_tag="JNZ73_004267"
     CDS             complement(<1..557)
                     /locus_tag="JNZ73_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY8734290.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPLSLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
     gene            complement(946..1212)
                     /gene="ospE1"
                     /locus_tag="JNZ73_004268"
     CDS             complement(946..1212)
                     /gene="ospE1"
                     /locus_tag="JNZ73_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HAY8734291.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(1321..1544)
                     /locus_tag="JNZ73_004269"
                     /pseudo
     CDS             complement(1321..1544)
                     /locus_tag="JNZ73_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          536 a          319 c          395 g          627 t
ORIGIN      
        1 atgctgtcta atctgtttcc agaaactacc agtaatttta gttccggtgg caatagtggc
       61 agaatatgca atcggttatt atgaacccgt aaatgagtta gattttcagg aagaaatggt
      121 aattctttta tcttgttatc acccgcatta agctcttcta acgtctcggg taaggcaggt
      181 aagctttcca gttgattaaa ttgagcatgt attaccttaa gcattggtgg taaatcaggg
      241 agatgtgtta ataggttttt tcttatttcc agcgttgtta tctgaggcgg taaatctggt
      301 aatgttttta aatctaactc agataaattt aattctgcct cttgattttg taaacatatt
      361 ttcagcctat tgaaggcgat attgcgttgc tcgcctgggg taccgttctt ttcccattcg
      421 ctccaggctt ttaaataaaa ttcattacaa gatggagtca gttccatagg taaagataat
      481 ggagttaacg agtatatatt gtttatttga tgcataatgc cagaaccgat tgcctgtata
      541 ttggttgatt taatcattat aaccagaatt cttatcaacg ataagaattc tggttaaata
      601 gaatatggct tattaatttt aaaacagttt gctttgtacc ctttctccgg gtattgcttt
      661 ccccctaagc cgtctatggg aaggagtctg ctgttattcc tgagaatggt aattcagcca
      721 taaaaaggta tttaaggtca ttgcgtctct ccctatactc gaataatggt gaagaaagca
      781 tgatgaatat atcacgataa cgttcagtac gtatcgagga tagatggagt tactcctctc
      841 cgaaatagaa ttaccgctct atttttttct gttttcagaa gttattagta acaaaccctg
      901 atatattttt ttgaaaatgg agcttaaacg agtctgaaat taacttcaga aatcagaata
      961 ccgttggctt tgtttttcat caattccggc ttcatttatc tttgtcaacc attgcatttc
     1021 tgtagcagtg aatttcaacg ttttcgtccg gctggcagca ggtatttgta attcgtatat
     1081 agctgctttt ttattgaaaa cagtatatcc atctgtagta acagtagaag ataataatac
     1141 aggatttgat acttcaagga tgatattttc ttgtttttgt ggaaggcaag ggaatatagt
     1201 ttgtgtaagc atattacttc cttatgttat attttggtaa aaaatttatt tttttacatt
     1261 atagtcggct aaatgaagtt tactgtaaaa aaacgatact ttttcgggtg gcgcttccaa
     1321 tcagtagaat atgtgttttt caatgaagga gccgatgact tttcgtggat ttccacggaa
     1381 cgcgagaagc agattgtttt acgtgccagc cgcttgatgc gggtgcggag ggtcaggttg
     1441 ttacgttcaa gatatagcat gtgctggatg aagatccctt ttctggtcac ctgtttatct
     1501 ttcgtggccg acggggtgac actgttaaaa tgtaatgaac gcatcccaca caattaaaca
     1561 cggagtagta tgatggttaa aagggtgaaa ctggcaggtg tggagcctcg cagctttccg
     1621 gcatcatact gcccaatacg ggatgcataa agcgtcctca gactggagac tccacaaatg
     1681 aaatatacac cggttggcgt tgatatggca aaacatgtca ttcagattca cttcatcaat
     1741 gagcacatgt cagcgccagt gatataagac ggtaatgggg atgcggacga tttcctggac
     1801 tctcagaaga tgaaacatgt ttactgaaga acaaaaaatt cgcgctattg agctttattt
     1861 caaatatggt aagaagc
//
LOCUS       DADMGC010000287         1852 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.290, whole genome shotgun sequence.
ACCESSION   DADMGC010000287 DADMGC010000000
VERSION     DADMGC010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.290"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..559
                     /locus_tag="JNZ73_004270"
     CDS             <1..559
                     /locus_tag="JNZ73_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010972061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8734292.1"
                     /translation="LGLARSSYFYHRAALKAGDKYATIRKMLTDITEFQLPAGKVWLS
                     PVVDCFDGKVVSWSLSTRPDAELVNTMLDSAVETLNAGERPVIHSDRGGHYRWPGWLE
                     RVNAAGLIRSMSRKGCSPDNAACEGFFGRLKTEMYYGRKWSGITPEKFMQQVDAYIRW
                     YNERRIKLSLGAVSPKMYRQQCGLE"
     gene            998..1835
                     /locus_tag="JNZ73_004271"
                     /pseudo
     CDS             998..1835
                     /locus_tag="JNZ73_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          454 a          454 c          565 g          379 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatcacggag ttccagctcc ctgcaggtaa
      121 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      181 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      241 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      301 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      361 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      421 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      481 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      541 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      601 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      661 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      721 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
      781 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
      841 cagtgtgccg gaaagcctgt gtggtcagct ggtatcaata cggatctcgc tggatgatga
      901 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
      961 cggctggcag accgtgccgg agcatcacgc tccactctgg cagcaggctt cggagcgcat
     1021 ggccgaacgg ctgggggaga tacagaaacg ggtaatcact gtctgtgacc gggaagcgga
     1081 tatctggcat tacctgtatt ataaagtcag ccacggacag cgtggtgcgt gctgcacaga
     1141 atcgccggct ggaagaggca cccggcaagc tcttcgaact gccggaagtc ctggcaaccg
     1201 caggaagcca cacgctgaat gtgatgcaaa aaggcgggcg ggcagcccgt caggcccgga
     1261 tgttcatcag ttacagcgaa gtcagcataa aaaatcccga caacagcggc caggcgctcc
     1321 cgctcacgta tgtctgttgc cgggagcagg cagaggacgg tgcctgctgg catctgctga
     1381 cgtcaggaaa agcggcgagt gccgccgatg cacgacgtat tgtcagccat tacgagcgac
     1441 gctggctgac agaggaatac cacaaggcgt ggaaaagtgg tggtacatgg aatcgctgcg
     1501 aatgcagacc cgggataacc ttgagcgcat ggtggttatc caggcgttta tagcggtacg
     1561 ggtgctgggc ctgcggcagg gaggtgtcag tgaagaaaca caaaatgaca gctgtgagaa
     1621 aatactgacg ccgacagagt ggaaactact gtgggttaag ctggaaggaa agccgcttcc
     1681 ggtgcaggca ccgaccctga aatgggcctg gggcgatggc atgacagcaa acgcacaggt
     1741 cgtcccggtt ggggcgtcat gtgggatggc tggtcagact tcaggatatg gttgagggtt
     1801 acctggtgat gaagtctctt gatcaggagc tctgatcaag agacagcagc tt
//
LOCUS       DADMGC010000288         1846 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.291, whole genome shotgun sequence.
ACCESSION   DADMGC010000288 DADMGC010000000
VERSION     DADMGC010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.291"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..356)
                     /locus_tag="JNZ73_004272"
     CDS             complement(<1..356)
                     /locus_tag="JNZ73_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY8734293.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGVCGGCP"
     gene            complement(356..1276)
                     /locus_tag="JNZ73_004273"
     CDS             complement(356..1276)
                     /locus_tag="JNZ73_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY8734294.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1414..>1846
                     /locus_tag="JNZ73_004274"
     CDS             1414..>1846
                     /locus_tag="JNZ73_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8734295.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAG"
BASE COUNT          460 a          454 c          427 g          505 t
ORIGIN      
        1 gggcaccccc cacacacacc cagcacgcca atgcgttcag catcaacata tggcagagtt
       61 actgcataat cgataacacg gctaatatcc tctacgcgct gtgtcggatc ttctatccaa
      121 cgaggctccc caccagattc tccctggaaa ctggcgtcat aagcgagaac gacatagcct
      181 ttttcagcca atgccttgcc atagatattg ccagacgtct gctccttaca actgccaaaa
      241 ggatggacgc tgataatcgc cggatactgt tttgactcat caaaatttgg tggaaactga
      301 atattggcag caatatgcca ggccatatca ctattgcgga tttgaatatg tttcattaca
      361 gagtttcctc aaagaacgga gctaatactg aaattgcttc agccacatag gctttgccgt
      421 cataaagatc catatggttt gcgccttcaa caatgtgata gcgtttatcc tggcttgatg
      481 cccgatcgta caggtcgtca ctcatccatt tgctccctgc ctggctgccc gccacaatct
      541 gcatcggctg agtcaggtac acttccgcca tatggtaagc atcataggta ataatctggt
      601 taaggctgcg caaagtagcg taacccggtg ctgttggata ctgcgcacga ggtgtgtggt
      661 aatactccca ggcctgacgc agttcttcgt tcggcgcatc ggcctccttc attggagcca
      721 gtggcataat ggcgtattct tcgctgctaa tatcgctggt tcgggcgttt gagccagctt
      781 caatgtgtgg cagagcatca atagatttca cattgttttc ccaaccattg cggaacatcg
      841 aaccaatatt gaccgcacta acggtaccga tggccttgat gcggcgatcc tgaattgcag
      901 cattggctgt atatcctgca ccggcacaaa ttcccatcgc accaattcgg gtattgtcga
      961 cataagaaag cgttgtcagg taatcaatca cggcactgac gtcttcagta cgaatgtatg
     1021 ggttttctaa ctgacgcggc tcgccgccac tttcaccttg ataagatgcg tcataagcaa
     1081 tagtgacaaa tcccttttcc gccagctttt cggcataggt tccggccgtt tgctctttaa
     1141 cgcccccacc tgggtgagat accacaattg ccggatactg acgggtttca tcaaattttg
     1201 gagggaaata gatcactgca gacaaagaga tggttggatt attgctgtta gtgaagctga
     1261 ctttattatt catcattcag ttcctcatgg agttgtcggt tcgttttgac ggttggtgat
     1321 atcactatag atattgatca ttaagttgat tagaccctaa atcatgatta gacctatcat
     1381 ttaaatgatt aatagattgt atggagatac tgaatgaagc gtgaagaaat cgctgatctg
     1441 atggcgtttg tcgtcgttgc agaggagcgt agcttcactc gcgcagcagc ccgcctgagc
     1501 atggtgcagt cagctttaag ccagatagtg cgccgtatag aagaacgatt gggattgcgg
     1561 cttctgacgc gaaccacgcg cagcgttgta cccactgaag cgggcgagca tcttttgtct
     1621 gttcttggcc ctatattgca tgacatagat tctgccctga catccctgag cgatctgcag
     1681 aaccgcccat ccgggacaat acgtattact actgtagaac atgcagcaaa aacgatattg
     1741 ttaccagcca tgcgcacatt cctgaaatcg catcctgaaa ttgatattca gcttaccatt
     1801 gattatggtt tgaccgatgt cgtttctgaa cgttttgatg caggcg
//
LOCUS       DADMGC010000289         1835 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.292, whole genome shotgun sequence.
ACCESSION   DADMGC010000289 DADMGC010000000
VERSION     DADMGC010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.292"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..222
                     /locus_tag="JNZ73_004275"
     CDS             <1..222
                     /locus_tag="JNZ73_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734296.1"
                     /translation="IANFIALQINGSKTGFAASITGFTQTISAGTISFLSVTVSDNGV
                     GPGTTLGLGSLITSLLSIAVIAIAGNNKK"
     gene            complement(864..1136)
                     /locus_tag="JNZ73_004276"
     CDS             complement(864..1136)
                     /locus_tag="JNZ73_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734297.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASGSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVLLLQLA"
     gene            1225..1404
                     /locus_tag="JNZ73_004277"
     CDS             1225..1404
                     /locus_tag="JNZ73_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734298.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGISAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            1456..1650
                     /locus_tag="JNZ73_004278"
     CDS             1456..1650
                     /locus_tag="JNZ73_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734299.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
BASE COUNT          582 a          422 c          360 g          471 t
ORIGIN      
        1 atagccaatt ttatcgcatt acaaataaat ggttcaaaaa caggatttgc cgcatctatt
       61 acaggtttta ctcaaacaat aagcgctggt acaatcagtt tcctgtcagt aaccgtaagc
      121 gacaacggtg ttggtcccgg gactacttta ggtcttggtt cactaataac ttcattactg
      181 tcaatagccg taatcgccat tgcaggaaat aataaaaagt aagtgtactc tgactgcgta
      241 tgtatctact ttgtgtaatg caggtgtcta ttgaaaataa ttcttttcgt gggcatctgt
      301 tgtataaaat aaaaaattat cgtaagtaca tctatctgcg atgtcttgtg aaaattatcc
      361 tgtctggcaa caatcgcgtc catctgtatt aatggacgcg aacgatgaac tctcaaacaa
      421 cactagatat caccggcttt cgcacttatt tgccagaatc attacgtctc agattcgacg
      481 agcaattatc tgtccgggct atcgcacagc agttaagact cagttattcc cccattcaca
      541 atctctttca acgaattaac gcctcaggta ttgtctgacc atgacccgag tccctttcat
      601 tcgcccaacc gataccatac tttatgccaa cagaaagaat gaatgaaccg cgcctaaaat
      661 cagcaaggac acatggcaca aagtgcgaca tgtctcacat ttaaccccag tgggggcaac
      721 aagtataaaa ccgttttttg attaagtggt cgaatatgaa aaggggaagg tttatgtgaa
      781 aaatacctgt gttttctttg aaaacacaca acagtatcag gttatttatg tttatacagt
      841 gcgttccaaa ctgacctgaa taattacgca agttgcaaca ataaaacatt tttgccgtag
      901 tgtgaggtac tccggtaaat tgctttccag gtgcagacaa tgactaacga agaactacag
      961 gcaatcgcat tatccatgct tgagcgccag cgcgaaaaag tacaccagca acgagagtct
     1021 ttgcttaacc catctccatt caccaggcca aaacgattgg agcctgaagc attcttggac
     1081 aaatacccac ggcggcttaa atcaggcaaa accagaccac caggctgata gctcacagtt
     1141 tgttctccga ttcaataaaa cggataagaa ccatcagcgc ggtagcgtaa ggcactacag
     1201 cgcattgctt tacgagtaat aacaatggat ttatcaccag aagacgcaaa gtttttacga
     1261 gaactgcaac accgtatgct cagagaagcc attatcgaac agcaaacggg cataagcgcc
     1321 atagacccat tgcgatacaa acgggcctca tctcctttag ttgacgacaa ttccaatgcg
     1381 gataaaacac gtccaccaca ctgattacat ttttcattag cacagtggtg tgatgcagca
     1441 tacgaggaaa accggatgaa tgagaacaaa ataaaaaggc tggagcaact gttacaggca
     1501 cgccaacggg agtttgccac taaatgggga cgtgccgcgc tgccacatga acgcctaacc
     1561 tattttccac ttgaagacct cattaaaaat aatggcaaaa aaatcaggtc tgataagaaa
     1621 cacttcttag aatgcaatga agacaaataa caacaatcta catattgcca ccaaccacag
     1681 atgtggcatc gttatcagcg cacttgattt tctttatatc cactgacgag cttgccgggg
     1741 cacctttgtt tgccggtcat tctggtatca tgcagatatt tacggcgtac aagcccacta
     1801 gcatgacatt gtaataaggc ctgagcctaa cgctt
//
LOCUS       DADMGC010000290         1784 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.294, whole genome shotgun sequence.
ACCESSION   DADMGC010000290 DADMGC010000000
VERSION     DADMGC010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.294"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..452
                     /locus_tag="JNZ73_004279"
     CDS             <1..452
                     /locus_tag="JNZ73_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8734300.1"
                     /translation="VGWNWNSGVYDANISGASTLILHFNKNTGSCPAVQFRVNYKNGG
                     IFYRSARDGYGFEAGWSEFYTTTRKPSARDVGAYTQAECNSRFITGIRLGGLSSVRTW
                     NGPGWSDRSGYVVTGSVNSNRDELIDTTQARPIQYCINGTWYNAGSI"
     gene            455..988
                     /locus_tag="JNZ73_004280"
     CDS             455..988
                     /locus_tag="JNZ73_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY8734301.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGIVI
                     KRTYTGEEQRQQVENEKQSLLQLVRDKTQLWDSQLRLGIISAGNKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1017..1544)
                     /locus_tag="JNZ73_004281"
     CDS             complement(1017..1544)
                     /locus_tag="JNZ73_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY8734302.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKNFDVIWLWTEDGKNWYGEVKNF
                     QPDTIKIVYDENNIIVAITKDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERSVRLNMATDEECARLESWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(1546..1784)
                     /locus_tag="JNZ73_004282"
                     /pseudo
     CDS             complement(1546..1784)
                     /locus_tag="JNZ73_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435977.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          498 a          392 c          401 g          493 t
ORIGIN      
        1 ctgttggctg gaactggaat agcggtgttt atgatgcaaa tatcagcggt gcatcaacat
       61 taatcctcca cttcaataag aatacgggga gctgccctgc tgtacagttc cgtgtgaatt
      121 ataagaacgg cggtattttt tatcgttcag cgcgtgatgg ttatggattt gaggctggct
      181 ggtcagagtt ttacaccaca acacgcaagc catcagcgag agatgttggc gcatatacgc
      241 aggcagaatg taactcaagg tttattacag gtattcgcct tggtggtctg tcatctgttc
      301 ggacatggaa tggtcccggc tggtctgaca ggtcaggtta tgttgttacc ggttccgtta
      361 actcaaacag agatgaacta attgatacaa ctcaggcaag gccaattcag tattgcatta
      421 atgggacgtg gtataacgcg gggagtattt aacgatgatg cacttaaaaa acatttctgc
      481 tggtaatcct gaaacaaaag agcaatacca gctaacgaaa caatttaaca tcaaatggct
      541 ttatacagag gatgggaaaa actggtatga ggaacaaaag aactttcagc ctgatacgtt
      601 gaaaatggtc tatgaccaca acggcgttat tatttgtatt gaaaaggatg tttcagcaat
      661 taatccggaa ggcgcaagcg tcgttgaggt tcctgatatt acagcaaatc gccgggctga
      721 tatttcaggt aaatggatgt tcaaagatgg catagtgata aagcgaactt ataccgggga
      781 agagcagagg caacaagtgg aaaatgaaaa gcaaagcctg ttgcaacttg tcagggataa
      841 aacccagcta tgggactcac agctacgact gggcatcatt tccgccggga ataagcagaa
      901 attaaccgag tggatgctct acgcgcagaa ggtcgaatcc acagacacct ccagtctgcc
      961 agtaacgttt cccgaacaac cagaatgaga gaaggcccgc tatcgggcct taatttttac
     1021 tctggttttt gtggccattc tggctttgcc gtatccacac ggctgaccag aacactgtag
     1081 cgttcccatg actccagtcg tgcgcattcc tcatctgttg ccatattcag cctgacagag
     1141 cgctccagcg gctggatgac tgattcagct tcggaaagca atgcggcttt ttgtgattcg
     1201 gcctgttgct gctgttcgtc tgccgtataa atccgcttaa ccacagctcc gtccttaaac
     1261 atccacttcc cggaatcatc ggcacgacga tttgctgtta tatcggggac ctcaacgacg
     1321 ctaaagcctt caggattaag cgtggaggca tctttagtga tggcgacaat aatattattt
     1381 tcatcgtaaa caatctttat tgtatctggc tgaaagttct tcacttcccc ataccagttt
     1441 tttccgtctt ctgtccacaa ccagattacg tcaaaattct ttgttaattc gtactgttcc
     1501 agtgttttag cgttacccgc tttaatgttc tttaagtgca tcatattaaa cgctcgctac
     1561 attataccaa gtgccattta tatacttttg aatcggtctg taataaacac ccgcaatatt
     1621 atcggcagag ttgaacctgt atcctgaaca ttaataccag acaatacatg acctgaaggg
     1681 cactgaaaat tccatgtttg ccagttgttc actccataat attgctgtga accaagtcga
     1741 acatctttca catatctgga atcaaaattg ccatagttgc cggg
//
LOCUS       DADMGC010000291         1772 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.295, whole genome shotgun sequence.
ACCESSION   DADMGC010000291 DADMGC010000000
VERSION     DADMGC010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.295"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..283
                     /locus_tag="JNZ73_004283"
     CDS             <1..283
                     /locus_tag="JNZ73_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734303.1"
                     /translation="KSDYLPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAG
                     IYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            285..889
                     /locus_tag="JNZ73_004284"
                     /pseudo
     CDS             285..889
                     /locus_tag="JNZ73_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
     gene            876..>1772
                     /locus_tag="JNZ73_004285"
     CDS             876..>1772
                     /locus_tag="JNZ73_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8734304.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRVF"
BASE COUNT          424 a          403 c          496 g          449 t
ORIGIN      
        1 aaaaagtgac tacctgcctt cagatattac ccgacaggta cagcaatccg tgctggacgc
       61 atttaatggt acagatggtg gtctgcgggc aagagttgcc tctgttgtct ctgccgggcg
      121 ctactatgcc ggcatttaca aaaccgatcc ggaacatatt gatattctgg gccttactgt
      181 gagccgtgac ggttcgtcat ggacaacggc tgtcactttc gggatagatg agattccggt
      241 tctggatgtg tcggatatca gtgtgaaact ccaggaggca taacgtgcag aatgtggctg
      301 ccactgtgct tgcgcagtat gctgccagcc cccgactcaa tgccctcatt aacagcttta
      361 acgcagcgct ttccccgaca gttttatcag tgatttttat ggtcttatct ggaacatcga
      421 taccgcagaa aagtatggtc ttgatgtctg gggaaagatt gtgggtgtca gtcgccggct
      481 gacggtaaag gacgatttta attacctggg cttcagcgag tccaggatgg acaccccggt
      541 aatggatgat ccctgtccgt ttaatcaggc accgttttac aacggcaaat cggatacccg
      601 gactgttgac ctgtctgatg ctgtataccg gcggctgata ctgatgaaag ccatgtcgaa
      661 cattactgac tgttccgttc cggatattaa cctgatgctg agatttatgt tcggaaaaaa
      721 acgccgggct tatgttctga ataatggcgg gctgaggatg agttacgtct ttgagtccgc
      781 gctctcgttg gcagaactgg cgattatcca gtcgtcgggt gcactgccat ccccgccggg
      841 tgtttatgtt tcagtagttt taaaggagtc ccgtaatgaa ggccagtgat aaaccccgcc
      901 agctggcggt cccctttgcg agtaccggag ataaaaaccg tatcccggac aaggcgacac
      961 agcagaccag agagagcggt aatgctgcgt atgattcagg ttttcctccg gtgaccatga
     1021 cagcggtctc agcgggaggt ataccgccac acggcaagga ttttaacggt ctgatgtacg
     1081 atattaccgc agcaatacgg ttcgcccagg ctggcggttt gtacacgtat aatgccggtt
     1141 ttgcgggggc cattggtgga tatgcaaaag gagccattct cgccggagtc gcaacaacag
     1201 cggtctggct gaataccacg gacgataacc tgaccgatcc tgaaggctcc gacagtgcgg
     1261 gctgggtaaa tcttcttgag gatccgaaaa ggatattcct gcggcagaag aacaatctgt
     1321 cagaccttca gaataaaggg acggcacggg ataatcttca ggtttacagt aaagagcagt
     1381 cagatcaacg ctatgttcat cgggaaggcg ataaaataac cggagagctg aaaatccgtg
     1441 gtgttaatgc gctgaggatt ttcaacgaag cttttggcct gatttttcgt cgttcggaag
     1501 agtgcctgca ccttattccc accagtgaag gtcaggggga aaatggcgat attggtcccc
     1561 tgcgcccgtt caccattaat ttgcggacgg gtgaaatatc catgtcgcat aaagtgtctg
     1621 ttggcggtgg ttcgcaggtc aatggtgcgc tgggtatcgg cgttcagaac gccctggggg
     1681 ggaattcaat tgttcttggt gataatgaca ccggatttaa acagaatgga gacggtattc
     1741 tggatgttta tgctaatagt cagcgtgtat tc
//
LOCUS       DADMGC010000292         1755 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.296, whole genome shotgun sequence.
ACCESSION   DADMGC010000292 DADMGC010000000
VERSION     DADMGC010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.296"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..289)
                     /locus_tag="JNZ73_004286"
     CDS             complement(<1..289)
                     /locus_tag="JNZ73_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HAY8734305.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGA"
     gene            complement(350..1165)
                     /gene="sul2"
                     /locus_tag="JNZ73_004287"
     CDS             complement(350..1165)
                     /gene="sul2"
                     /locus_tag="JNZ73_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAY8734306.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT          419 a          474 c          470 g          392 t
ORIGIN      
        1 ccgctccaga cagatcagcc gccggtactc ccggaattgc cgttatcacc aagcatgcac
       61 cctcctgttc ctcctgccag ttgatgacct cggggcaagc cacacctcga cctttgagcc
      121 aaatgaggcg gtcacgctct ccagcgagct caccgcggcg ggaagcaggt gcgattttcg
      181 cgaaggcatg cccgtcacca cgtcgaaaaa caaaatcacc agattctccg cctctgacag
      241 gcaaccagtc agaatgcgat tcaccaaaaa aaatattagt tcgattcaat ggaggttcct
      301 tcagttttct gatgaagcgc gaatatagag aaatatcccg aatgtgcagt taacgaattc
      361 ttgcggtttc tttcagcgcc gccaataccg ccagcccgtc gcgcaagggg cgcggctcgt
      421 gtgtgcggat gaagtcagct ccacctgcgg cggcggcaag ctctgcagcg agtgtcgcgg
      481 ccccgacatc ccccggacca cggcctgtga gcgcgcgcag aaaggatttg cgcgaaacag
      541 acagaagcac cggcaaatcg aagcgcagcc gcaattcatc gaaccgcgcc agcaccgaga
      601 gcgaggtttc gggagcagcc cccagaaaaa accccatgcc gggatcaagg acaaggcggt
      661 tgcgtttgat accggcaccc gtcagcgccg cgatgcgcgc gtcaaagaac gccgcaatgt
      721 gatccatgat gtcgccagcg ggtgcctcgc gccgatctgc ctgcccgtct tgcaccgaat
      781 gcataacgac gagtttggca gatgatttcg ccaattgcgg atagaacgca gcgtctggaa
      841 aaccgcgaat atcattgaga taggccacac cacgcgacaa ggcataggct tgcgtcgcgg
      901 gttgataact gtcgagcgag acgggaatgc catctgcctt gagcgcgtcc agcaccggcg
      961 cgatacgcgc gatttctgtg tcggacgaaa caggcgcggc gtcgggattg ctggatgccg
     1021 gaccgaggtc gatcacatct gccccctcgg ccatcagctt acgcgcctgc gcaatggctg
     1081 cgtctggcgc cagataccgg cctccatcgg agaaactgtc cgaggttatg ttgacgatgc
     1141 cgaaaatgat gagcgattta ttcatggggg cttctataat aataataatc gagcatgagt
     1201 ctcatacgga tgctcgggtc gaaagggaat ccccaggcga gtaacctgtt tgcggtgatc
     1261 cgtttcatga tgatgtatct gtagcatttt ggaaataaat atatcatctc tcaataagta
     1321 gatatattta tcttttttgc ttctttggtg ctgcaagtca ccaatacgag ggaaacctgt
     1381 tacggagcgg tttaaccgta atcaacagtg actggtgtca tgttcggaag attaattgat
     1441 agtaatctac aaaggatctt cttgagatcc ttttcaatga atcgtaatct cttgctctgt
     1501 aaacgaaaaa accgcctggg gaggcggttt gttcgaaggt taagtcagtt ggggaactgc
     1561 ttaacccggt aacagggttc acgagaccgc cattaccaaa actgttcttt cagtgtagcc
     1621 gcagtcaggc ctctacttca agacctctta atctatcttc tcgtgcactc ctgttactaa
     1681 tggctgctgc cagtggcgtt ttgtcgtgtc tttccgggtt ggactcaagt tgatagttac
     1741 cggacaaggc gcagc
//
LOCUS       DADMGC010000293         1748 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.297, whole genome shotgun sequence.
ACCESSION   DADMGC010000293 DADMGC010000000
VERSION     DADMGC010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.297"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(1..262)
                     /locus_tag="JNZ73_004288"
                     /pseudo
     CDS             complement(1..262)
                     /locus_tag="JNZ73_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(377..1015)
                     /locus_tag="JNZ73_004289"
     CDS             complement(377..1015)
                     /locus_tag="JNZ73_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734307.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1174..1644)
                     /locus_tag="JNZ73_004290"
                     /pseudo
     CDS             complement(1174..1644)
                     /locus_tag="JNZ73_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          497 a          432 c          316 g          503 t
ORIGIN      
        1 gcaagcgctg taactctttt atttgtttta tagcggcagc cagttctgag gcaggaacga
       61 catcctctcc ggcagcaaca gcagtgagac ttccttccag atattatttt ttccacttaa
      121 gcaacaggct gggctgaata ccatgcaaac gcgccacatg ggacaccgtc atgccaggct
      181 ccattgtctg ctgaataatg gcaatttttt cctgaggagt tttacgttta cgaagctcca
      241 gccccagcaa agttccagtc atctcaaaat tagaactagt gttagacata tatccaagcc
      301 tatctcttat tttaagatac atccactgtc tggcgtttca aggggccggc tcaaatctat
      361 gtacaggctc tcagcatcag caaagcctct tacttttcca actactactg ttagcatagt
      421 gatcaaattc ctgatcagat acatgaatta cctgaaccga actattttca tctacaaatg
      481 catctggcga tttttctaca ggatcataaa gattaaatcc ctttcctgtt tgaacatcca
      541 aaaacataat tttttcacca tcgttaaatc cagctatcca atgagtggcc tgttcaatag
      601 aaatcattac tacacttcct tttgaagtat tttgtaaaat attttctgca atacgctcac
      661 gaaatttact ataagtttca ttaggatgtg acactatgcc ttcactatca atttgctcta
      721 aatgcctatg aaaaaatccg gtcatatact ctgatatttc attaggtacg ggagcctctc
      781 ttactccata ccctaacata gccatacaag catgaagcgc acaccctgaa cagttaccat
      841 ccccaccaga cggatttatc attttctgta aagtcgctgc caattcagaa tctttatcca
      901 tatcaccaag acttttagag tttaaagatt ccagcaattt tttaacattt acatttccct
      961 cctctgtatg tgcctcatat ccagcagttc cctgtaacga caccccatta atcatattgt
     1021 atcctgacag tatatatact cactatattt tcttcataac aaatatccct ctgatatgac
     1081 aacaaaaaac ggagccggat tcctgttttt gtgaactgca tctattcgtg tctaaaaagt
     1141 gatccactta ccaccaacgg cctaatatat acagaacgga gtaaacaggg ctaacagttc
     1201 ccgacaggtc tcatcattcc gtggcccgaa agtgtaagcc aacacaccgc cagttttagt
     1261 gttgtacgca taccagagcc agtgttgttg cagccttgct gccaacgaag cgccactgct
     1321 catcaagttc acagataagg gcaacatcag cgtgagcgac tggcgcagat gttatcctcc
     1381 ggggcgggag ctttttaatg tccgtatgac ggtattgatg ccgattttca gtgtcctggc
     1441 ggtatcacgg ccccctgcgc cgttatgtgc catttcaacg atctgctcct taacgccggg
     1501 ttttctggct tcataagagt aagtgagctg gaaaacacgt ttacatgagc gacaacggaa
     1561 acgctcatgc cgggaacaac tacggccatg tcgataaact tcatcggaat gacagcgcgg
     1621 acaatgaaca gtaacggtag ccatggaaaa ccttaaatac cagtagatta ccagaaactt
     1681 ctgtcgattg aagacatcac ccgaagtttg aattcattcg aatacttttt acgaggttct
     1741 tttcgcca
//
LOCUS       DADMGC010000294         1740 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.299, whole genome shotgun sequence.
ACCESSION   DADMGC010000294 DADMGC010000000
VERSION     DADMGC010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.299"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..366
                     /locus_tag="JNZ73_004291"
     CDS             <1..366
                     /locus_tag="JNZ73_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8734308.1"
                     /translation="VYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVV
                     CVQQPMFTEMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAIYLL
                     AGCLISAMTALLMKDNQRS"
     gene            468..>1740
                     /gene="amn"
                     /locus_tag="JNZ73_004292"
     CDS             468..>1740
                     /gene="amn"
                     /locus_tag="JNZ73_004292"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY8734309.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVSYGTLL"
BASE COUNT          411 a          436 c          410 g          483 t
ORIGIN      
        1 gtttatatca caggcgcgtt gatcggaacg ttgagcgcat ttcctttctt tatggcgctt
       61 gaagcacaat ctattttctg gatagttttc ttctccataa tgctggcaaa cattgcgcat
      121 gacatggtgg tgtgtgtgca acaaccgatg tttaccgaaa tgtttggtgc cagttatcgc
      181 tacagtggtg ctggagtcgg ttatcaggtt gccagtgtgg ttggcggtgg atttacacct
      241 tttattgccg ctgcactcat cacttacttt gccgggaact ggcatagcat cgccatttat
      301 ttgctggctg gatgcctgat ttctgcaatg accgctttat tgatgaaaga caatcaacgc
      361 tcgtgatagc ctgccgaaga tcatccgatc ttcgccttat acttttgttt cacattcctg
      421 tgacatacta tcggatgtgc ggtaattgta tggaacagga gacacacatg aataataagg
      481 gctccggtct gaccccagct caggcactgg ataaactcga cgcgctgtat gagcaatctg
      541 tagttgcttt acgcaacgcc attggcaaat atattacaat tggcgaatta cctgatgaaa
      601 acgcccgtaa acaaggtctt tttgtctatc catcactgac cgtaacctgg gacggtagca
      661 caacaaatcc ccccaaaacg cgcgcatttg gtcgttttac tcacgcaggc agctacacca
      721 ccacgattac tcgccctact ctctttcgtt cgtatctgaa tgagcaacta acgttgcttt
      781 atcaggatta tggtgcgcat atctctgtgc aaccctcgca acatgaaatc ccttatcctt
      841 atgtcatcga tggctctgaa ttgacacttg atcgctcaat gagcgctgat ttaactcgct
      901 acttcccgac aacagaactg gcgcaaatcg gcgatgaaac tgcagacggc atttatcatc
      961 caacggaatt ctccccgcta tcgcattttg atgcgcgccg cgtcgatttt tccctcgcac
     1021 ggttgcgcca ttataccggt acgccagtag aacattttca accgttcgtc ttgtttacca
     1081 actacacacg ttatgtggat gaattcgttc gttggggatg cagccagatc ctcgatcctg
     1141 atagtcccta cattgccctt tcttgtgctg gcgggaactg gatcactgcc gaaaccgaag
     1201 cgccagaaga agccatttcc gaccttgcat ggaaaaaaca ccagatgcca gcatggcatt
     1261 taattaccgc tgatggtcag ggtattactc tggtgaatat tggcgtggga ccgtccaatg
     1321 ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga tgtctggttg atgattggtc
     1381 actgtggcgg attacgtgaa agccaggcca ttggagatta tgtccttgca cacgcttatt
     1441 tacgcgatga ccacgttctt gatgcggttc tgccgcccga tatacctatt ccgagcattg
     1501 ctgaagtgca acgtgcgctt tatgacgcta ccaagttagt cagcggcagg cctggtgagg
     1561 aagtcaaaca gcggctgcgt acaggtactg tggtaaccac agatgacagg aactgggagt
     1621 tacgttactc agcttctgca cttcgtttta acttaagccg ggccgtagca attgatatgg
     1681 aaagtgcaac cattgccgcg caaggatatc gtttccgcgt gtcatacggg acactactgt
//
LOCUS       DADMGC010000295         1702 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.300, whole genome shotgun sequence.
ACCESSION   DADMGC010000295 DADMGC010000000
VERSION     DADMGC010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.300"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            1..649
                     /locus_tag="JNZ73_004293"
                     /pseudo
     CDS             1..649
                     /locus_tag="JNZ73_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052544.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            649..1383
                     /locus_tag="JNZ73_004294"
     CDS             649..1383
                     /locus_tag="JNZ73_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HAY8734310.1"
                     /translation="MVNDLTYKKLQSTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRNERCNNMRTKQKV"
BASE COUNT          477 a          314 c          418 g          493 t
ORIGIN      
        1 gctatcaggc gtttggctgt gaccgtgtat atgccgcctg aaaatgacaa acaataacgc
       61 gcaccatcaa tgtgaaaagt ggtttattgc cttaatgggg ttgcctttag tcattcatat
      121 gatgaccgaa tatcctctct atacgtctgt tccccatttg atgctgttgc tgctcctttt
      181 ccgcatcgga atgccggaaa acgtgatgaa tcaggtacgt atacctaaaa cagtccggtt
      241 gggtattctg cctgtcattg tgctcattat gaccgccgcg ctggtcatcc tggtagcggg
      301 atttgatgca cagcgggaac tgaccagagc tgaagctgat atgaacgaag gatttatccc
      361 tcgccttccg gaacatagct ggaaatacct gacgcaggca gaacgtcttg agcgcgatca
      421 gcatctgctt attgcgaata aaccggggtt cgttcgcgat acggaaagca tgtctctttt
      481 cactgtgtgg ggaaataaat ggttggcaat ccacaatgat gcagacgtaa gtgctgcaat
      541 gatattcatt gcgcacaggc gcggtgatta tctgacagct gaaaaaataa gaaaacgcgc
      601 agctcgggta tttgtaaatg acgaacgttt tactcgcggg ggaaactaat ggttaacgat
      661 ctgacgtata agaaattaca aagcacaaac atcaactgta atattctctc gccagaaaat
      721 accacatgga caaatgtcac ggtgattggc ctggatcttt ttgccatttg cggcgtgtgc
      781 agtttgctca agcgtgttgg gggagtgaaa cttcaggttt tcacttcctt acccactgat
      841 atgaatgttc tgactgacag tagcatcgtg atatgggtaa aaatgcgtca cgatggtttg
      901 cctgaattag ccggacatat tgtgaaggtt tgtcgtcgat accgtctgat gcggcaactc
      961 gttattagcg atgctattcc tgaaggaatg acgaatgata tcgggcctct ttcccgagta
     1021 tggcttactc atgggagtgc aaaagtggat attttattat cttcattacg gaaggtgtta
     1081 aatgctcatt gtttgcctgg tcctttgttt ataaaacgcc tgggaagagt gcaatggagg
     1141 gttcttttac tccgcgcgaa ggggattagc acacgtaaaa ttgcagaaat ctgtggtatt
     1201 ggcctcaaga ccgttagcgc ccatgagtcc gcaattcgag aacgattagg caccaggaat
     1261 aaaagtgagt acgtctggtt agtacgtaat gctcaactta tacaacaggc gatacccgcg
     1321 ttgggaagag atgcgtcaag gaatgaaaga tgtaacaata tgcgaacaaa gcaaaaagta
     1381 taattgatgc gtcatagata actctctttt tcgatgacga aatcgctgag catgcgtggt
     1441 gaggttagta atagatgggt gctatgtttt gccacgggtt tgtagctgtg aaacctggaa
     1501 aaaaataaga attaaaaata tttaaagaga gttaataagg ttctggttgt aattgtcttg
     1561 gtcaaaaaaa agatattgtc aatgttgttt tttaaatttt caggttttgg ttaaatattt
     1621 gcttacaaat ataggtgatt tttttattgt aggcttctga aggctattat aactgtgcgc
     1681 taaattaatg cgttgaataa aa
//
LOCUS       DADMGC010000296         1701 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.301, whole genome shotgun sequence.
ACCESSION   DADMGC010000296 DADMGC010000000
VERSION     DADMGC010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.301"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(603..939)
                     /locus_tag="JNZ73_004295"
                     /pseudo
     CDS             complement(603..939)
                     /locus_tag="JNZ73_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          552 a          338 c          329 g          482 t
ORIGIN      
        1 aatgtgtgtc atacactcag tggcgtaatc gttgacaata tggtgcatgt aatcgtaagc
       61 gtccagcgaa atccatctgg tcataatctg aagcatccga caaagtggtg tccaccaaat
      121 aagtgatctg cgcccaatat cctgagccgg aactttgaca aaggccaacg aaaaatggga
      181 gcggatgtta cagagttctc ggtacagggg aaaagtggta cctgtcgcca gttctccgat
      241 ctgtttaacc gggaaattat ctcctacagc ttgtcagaaa ggtcggtgat ggagcatcgt
      301 taatacgatg cattcacagt gcccggccag aggatgcccc gtcgctgcat atggatcagg
      361 gttggcaata tcgaatcgca ggctataggc aaagttaaag ccccatggag tagcacaaaa
      421 tatgccgcac aaaggaaacg gtctgaataa cgcagtgatg aagaacttct tcagcacact
      481 gctaaaacgc atagtgatcg agcgctgatt ctggcgaaca actgaactaa tacatcagaa
      541 tctgcattat gttaaataaa tatgaaaaga tggtttaaat accccgttac ttttgactta
      601 cactatacgg tatcgcatcg tttaatattc gcaccggcca gatttttatt tctattagtt
      661 gtcacaatac tgaatgcgta cgaccacagt attctggctc ctgtgtggtt atgctttaat
      721 tctgcgttcc gggcagataa gcagttgctt gcaggaatcc ttcttgtgtt aatgtcagtt
      781 ccccttttta ccagtgctga tttccacatt ccgtccaaca gagcttatag cctttccctg
      841 gattatagca ttgtccggct gaagttcttt ttgaataata atagaagcac tgctggcaga
      901 tccagtccgt ttttcataac ccactgtact gataaccata atctaatcag tagaaattga
      961 gtcgaaaata agcactactc catacaggat aattagaggt cagtttgatt attcacaatt
     1021 catcatcagc attttctatt tctgacgaaa tcaacatgaa aataaccata tatgataatt
     1081 attataataa cggctttaat tggaatacat atattacaac gtattatata taattggtat
     1141 tctgggaact atattctcaa aatacagtag aaacgaggta tgtttctggt ggaaaggaca
     1201 gtgggattaa aaagtaagaa ttgataaaaa aaacgccagc aacacatagt gccggcggag
     1261 ggaatacccc atggagaaaa tgtgatgcct agagcatcat gaatatataa ttaaaaatag
     1321 ttagcgttgt caatactttc acaaaaataa ttttcgtagt atagaaagat atttttatgc
     1381 atgacctacc tgaatttgct ccgggtagag gttataaata aaaattgaat cacgacaaac
     1441 acaatattca tagtatggcg atgcctacgc cagcaagaat agcgccaata atattgggaa
     1501 tataatagac accagacgca caggcatctc actccttaac aaacaataat caggatattt
     1561 acttttaccc agctaactgt ttacacccaa agtgcacaca attaaccatt caataacaaa
     1621 tggcaatatc catagccata cgactttacc ttgtaatgtt cggtatttct ttataattat
     1681 tctgggaaat ctatcattta t
//
LOCUS       DADMGC010000297         1696 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.302, whole genome shotgun sequence.
ACCESSION   DADMGC010000297 DADMGC010000000
VERSION     DADMGC010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.302"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            245..1200
                     /locus_tag="JNZ73_004296"
                     /pseudo
     CDS             245..1200
                     /locus_tag="JNZ73_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842986.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1197..>1696
                     /locus_tag="JNZ73_004297"
     CDS             1197..>1696
                     /locus_tag="JNZ73_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HAY8734311.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASVAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGR"
BASE COUNT          463 a          410 c          470 g          353 t
ORIGIN      
        1 ctcacttatc gatcaactga taacggaagt catactgagt tcacactcca gagctgcggc
       61 actggcggtt gattctgccg ccgcaaaact gatgcagaat gcagacaata tcagtgaata
      121 cctgtcgtta caccatcagg ggctgatgca aagtattcag tcactgcagg cgcatatcac
      181 cagcctgctt gaggatatcc gggaaatagc agactgtcag gagcaggtga ccgctgatgt
      241 cagaatggcc atggaggaga tcgatgccag aacccgggaa ttactgacgg ggtctgcacc
      301 tcactggaag aggagctgaa tgactatttc agaagcggta aacgcaaaga acagcaaatg
      361 ctggaggaag aaaattcagc gcagccccgg gagagaaacg cattcgcttt ctttcacgat
      421 attttcggaa ccggaaatca gcatgacagg atgcgggact ttgatccgga cagccccgaa
      481 ataaaattca gtgatcgcag ggaagccctt gaactgatga cgcaaatcga atcgaccgtg
      541 accagcctgc accgagaggc tgaagcacag ttccggcctg agctggagaa aatcgtcagc
      601 gggattgaaa caggttttcg tggcacggcc ctgtacgcca cagaaaacat tgccggtcgc
      661 atcaataccc gcctggagga tgagggcttt accgtaaaaa tcagttttcc ggcagtcagt
      721 cagttacaaa cccggctcgc ggtaaaaaca aatctgagtg cgcttatgga ggaaagaacg
      781 gagaccgtca cccgtcgccg tcggcagcgt ggtttatggg gaaagatttg tggagcgttt
      841 ggcaccagtg actggggctg ggaaacctat aaagaggatg tgagccgcag tgttatcaat
      901 atcaacaccg tcaggaagga agttatgtca ctgacccggg catatttcgg ggagctgcag
      961 gcatccattg agcaggatat taaccagccc gtccgccagg aaatcgatgc ctttttctgc
     1021 gcattcaggg agaaagttga acaactgcgt aacacgctga ttcagagctc tgaagatcat
     1081 aaacgcgatc agcaggcaca ggaacggctt accaggcgac ttcaggcatt aaatgaaagg
     1141 gttcctgagc tcattactga cagtaaggcg ctgagggaag agctggagac aatgctgtga
     1201 cttcaccatt tattcaacaa attgctgata accgggtatg tcaggtactc acctgtcttc
     1261 ctgaaaaatt tgtggttgat ttcgcaaacg gaatcgatgt tgcacaggag catatccgca
     1321 cggccggaga gcgcacgttc ttccggcgtt taaaagaggg actgaccggc gaaggcgctg
     1381 cacggcagaa tgccatcaat gcttcggttg cacagggcgt tgaggcgtcc ctgcgctggc
     1441 tgacggagct gacaacgtca cttgccacca caaactacgc gattacccgg gtaaacgaca
     1501 gggtcagttc actggtcagt gatactgcca ggttggcgca ttattccgca gatacacggg
     1561 agcagttact catcctggcc gatcaggttc accataaact gaatcatctt gaagaaaaac
     1621 tccaccgtgt tgaccaggtt cagcgggcgc agttacatct tgagcagata ttctcatggt
     1681 ggagcgccgg gcgata
//
LOCUS       DADMGC010000298         1678 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.303, whole genome shotgun sequence.
ACCESSION   DADMGC010000298 DADMGC010000000
VERSION     DADMGC010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.303"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(71..538)
                     /locus_tag="JNZ73_004298"
     CDS             complement(71..538)
                     /locus_tag="JNZ73_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAY8734312.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(604..843)
                     /locus_tag="JNZ73_004299"
     CDS             complement(604..843)
                     /locus_tag="JNZ73_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HAY8734313.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(978..1583)
                     /locus_tag="JNZ73_004300"
     CDS             complement(978..1583)
                     /locus_tag="JNZ73_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8734314.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          541 a          370 c          293 g          474 t
ORIGIN      
        1 agacctactg ctatcacaat tactgagaga aaaaaagcat ccgatgattc atttatgctg
       61 tcctaaaaaa ttaacaaaac aatgggaaaa caggatatgt aagcagaaaa gccaatgaaa
      121 ttggtacagc ataaggcaca ccgttttcac gaattgattt tcgaaaaaaa agaagacctg
      181 ccagaacaac cagagcgccg caaaaggtca ttgcgacgag aaaattagcc agttcatgtt
      241 catcaatagc taacgaaaga acagttaaca gcttgacatc accgccgcca attatccctg
      301 cggaaaaaag aaagaagccc acgagcaaaa cgctcccggc tgcaaccaga ttgggttttt
      361 gatgctgtaa aatgagtgcc aaaaaaataa gcacggttaa caccagtaaa taacgatggc
      421 taataattct gagtaatata tctccccatg aaataaaaat aagaaagagc gcaacaaata
      481 aaagatcgac tcttagtaac catattgctg ccattgaaag actccatgta ccatacaagg
      541 ttgctgccag acatattcct ttacgtctga cagcctctag agagtttttt tattttttat
      601 aagttacgga ttactcgttc cggaaatcgt tacctgttga attgctgcag cgatagcgtc
      661 gaatgcatct ttaattgctc ccaggaagcc tgagttctga tcgccaaaga tgaaagcaag
      721 cagagttgcc atagcaacac caatcaatgc atattcgatt gcagtaacac cacgttcatc
      781 cttcgcgaaa cgcgcaaagc actgtttagt tttaccatag tacttagtaa taaaagtatt
      841 catgttttcc tcgtatatta ttatttaaat tcaaaccacc agcttcatct gcgtgtcacc
      901 ggtatcccat atttttggga tgtggctata attatccatt cccgctattt agtcttcaaa
      961 aaaaattaca tttaatatca tttatcttga ttaagaaata gtaagactaa aaaaacaagc
     1021 gtcgtcagcc agcacactgc taacacggta agaacgctaa taaaaacact ccactgcttc
     1081 gttctatacc attcaatatt tttgtcagta tgttttattg ggagcagctc ttcagtagag
     1141 aattcctggc tgttatcatt ctcaggaatg tttgcatcag tgtttacttc cctggcctcg
     1201 ctatcgttgg tatagctact actgtcttca tcactgtcac gctcattctc acagacatgt
     1261 ttctcgacct ttcccagata tctgacgcct tttcgcggaa tggttttaat tagagaactc
     1321 gacaaacctg cgacgtctaa tgcctttcta agcatgtaaa tctgcccata ataactgctg
     1381 tcgcttaccg aacctttctg ctcccgccaa acctcattga taatatgttg cttatcggat
     1441 tctccctgta gtaaaaggag taaaaatcga tagccgtttt cagacagatt gattacattg
     1501 cctgtactgg tggtgatacg tcgagacgct gggtaaaact taactccctg ccctaaagaa
     1561 aaataaaaac ggtcatcgct cataagtacc gaaccctgct ttcttcttca catgactact
     1621 cattacaact ttgtatgaag taagatgcaa aatcatattg tttacattaa gttaagca
//
LOCUS       DADMGC010000299         1619 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.304, whole genome shotgun sequence.
ACCESSION   DADMGC010000299 DADMGC010000000
VERSION     DADMGC010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.304"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(821..1060)
                     /locus_tag="JNZ73_004301"
     CDS             complement(821..1060)
                     /locus_tag="JNZ73_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY8734315.1"
                     /translation="MPQGGVISPLLSNIILNEFDQYLNKRYLSGKARKDRWYWNHSIQ
                     RGRSTAVKENWQWKPAVAYCCYADDCVPRRRVLGT"
     gene            complement(1255..1619)
                     /locus_tag="JNZ73_004302"
                     /pseudo
     CDS             complement(1255..1619)
                     /locus_tag="JNZ73_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          363 a          450 c          386 g          420 t
ORIGIN      
        1 tcgtcaaaaa aatcatcttc gtgaagtttt acgacatagc ggaggagttt gcttctttga
       61 ggtgcaggtt ttcgtttgcg tggtttagct gaacgggcca tataactacc tgaaagacaa
      121 tgatattgtc tgtttttata acggtaatca caggccatga caagccgcag ccgtcaggcc
      181 gcctactcgg ggcggtttag aacgcttatt tagtaccaga tcttacttat aataaagggg
      241 cttggcagtt tggataaact attgtcctta ttccactaat acaaactttt tgtaaagtat
      301 attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc
      361 ataaaaggaa gagggggccc caattaagtt gtcaagcatg tttactgcgc aatcgcttgg
      421 tgctccgtcc ctgtaccccg cattggtact ctggcgctca tggggcttcc acttgcgctt
      481 ctcccttcac atcggggtga caggtccacg ttccccacaa gagcctgaat cgacttcacg
      541 ccgcttttat gccggatgcc gcgcaggcag taaacatatc ccacaggaac gactccacca
      601 ttgttttgac atcatctata tgctttcgac acatcatcag cggttcactt tcgttcgtct
      661 cttcgaacca tacctgatcg gctcacgtcc gaccttttcc catctcgctc accaccacat
      721 ctcttaaatg cagcagcagt ggatggtttg gtgcctgcgc ctgcacgccg acaccgaggg
      781 gccttccctc atctcttgtg ctgcatggct gcacagggac tcacgtcccc aggacgcgcc
      841 ttcgtggcac acagtcatcg gcatagcaac agtaagcaac ggcaggtttc cattgccagt
      901 tttctttgac agcagtactt cggcctcgct ggatactgtg attccagtac cagcgatcct
      961 ttctggcttt tccactcagg tagcgtttat tcagatactg gtcgaactca ttcagtatga
     1021 tgttcgacag caacggcgag atcacaccgc cctgtggcat actttcactg accgcccgga
     1081 agagaccaac atcaacacgg cgttttccac agcagtctca taaaacgtgc atcactgatc
     1141 ctgcggcaga cggctttcat cagcagccgg tgatgtactg tgtcgaagta gctggacagg
     1201 tcaccttcaa taacccggcg ccccgtgttt ccccgcagtc ggtgagctgt aatagtacgt
     1261 gatgtctccc gcccacttct ggttcgggcc actggcgtaa aaatcctgcg tcagcagatt
     1321 ttctgataca ggcaggccgt gtgcacggta actgatcggg ctgaacttct gggaggcttt
     1381 tgcgctcagt tcctgacggt gcagactggc tgccacggtt tttacgttaa agtggtaacc
     1441 agagcacaca gttcatccgt caggcgtggg gcaccgtaac gctggactgg gtaaaagcct
     1501 tgcggacaac gctgtcgcag tgctgacgga actgctgacg tgtgtttatc cttgtccgcc
     1561 gctgacacca cgtataccag ccgctgcggg ccacccggag tacgcggcac attgccttg
//
LOCUS       DADMGC010000300         1590 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.305, whole genome shotgun sequence.
ACCESSION   DADMGC010000300 DADMGC010000000
VERSION     DADMGC010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.305"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..680)
                     /locus_tag="JNZ73_004303"
     CDS             complement(<1..680)
                     /locus_tag="JNZ73_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY8734316.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPEFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
     gene            complement(996..>1590)
                     /locus_tag="JNZ73_004304"
     CDS             complement(996..>1590)
                     /locus_tag="JNZ73_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY8734317.1"
                     /translation="WWVLGVSNRLAWGCPTKEHLLPHFLEHLGNNHLDIGVGTGFYLT
                     HVPESSLISLMDLNEASLNAASTRAGESKIKHKISHDVFEPYPAALHGQFDSISMFYL
                     LHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFS
                     NTKDSEEGLTHILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          468 a          296 c          331 g          495 t
ORIGIN      
        1 aacaaagttt gcattattct gggagatagt tgattttctc taagattgac aacgctatca
       61 gaactgaggc caagtatgct ttccggaatt gccgtgatcc ggttctgatt aagccagaat
      121 attcttataa tatgatcatc tctttctgga aactctggta ttacttccaa agcatttctg
      181 gctgcatcaa gtaattccaa tgacattggt aacctcggaa gtacagacag gtgattgtca
      241 cttacattaa tatattctag tgacgcaggt aattcaggaa gtgcgaacaa atggttatca
      301 ctcacattta tatattccag cgacactggt aattcaggaa gtgcagataa ttgattactg
      361 cttgcattca gctctttcaa tgcccttggt agctcgggga gcattgatag ttggttattg
      421 cttacattaa tttcatcaag attgtcaggc aatcgtggta gagatctgag acctaaacaa
      481 gataagtcca acgatgtttc actgttctcc agacataatt ggagccgggt aaaagcagtt
      541 tccctgtttt ctccggaaat gctgttttta gtccattcaa cccattcgga gagataatta
      601 ttgtgaacat tgtcgattga tgtagttctg taaaaagaga cgtttccagt ggataggggg
      661 ggattattta caggaagcat aataacctcg cagaaggata tcctgataaa atgtgatact
      721 ggggaataat aagctgaaat aaaatgtttc aggattgaaa tttattgttt tcattttaag
      781 gaagtgatgt tgatttaact gtaaaaaaac gatactattt tcttagatgg taaatgtctc
      841 gcctgtgcta tattttgttt ttcggttagt taaatatcgt acgttcatat ttgaacgttc
      901 tgccggaatg cattatcaat agaggtaaag tcgcaacccc aaatcgtaaa ggaaaccgta
      961 gcacgtctta agcaagaacg tgctgcggtt ggatgctatt ttttccctga agcggaaaac
     1021 attactacag taccttgaac cttggtttta acattctcga aatgctctga gagtatatgt
     1081 gttaagcctt cttcggaatc ttttgtgttt gaaaagatgc ctttctgatt gtaaatgcgc
     1141 atcagttttt gaccgaagct attgtgcaca actccatcgc caagaattgt ggctccgtat
     1201 agagttccat catcagttaa ggcctgcgcc gcattgcgta ttacacagct ttttgtagat
     1261 atatttccag gcaggcagtg aagaaggtaa aacatggaaa tggaatcaaa ttgaccatgt
     1321 aacgccgcag gataaggttc aaaaacatca tggctaattt tatgtttaat ttttgattcc
     1381 ccagcccttg tagatgccgc gttcaggcta gcttcgttca aatccattaa agatatcaga
     1441 ctactctcag gtacgtgagt aaggtaaaac ccagttccaa caccaatatc cagatggttg
     1501 ttacctaaat gttccagaaa gtgtggaaga aggtgttcct ttgtaggaca tccccatgca
     1561 agccgatttg atactcccaa aacccaccag
//
LOCUS       DADMGC010000301         1489 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.307, whole genome shotgun sequence.
ACCESSION   DADMGC010000301 DADMGC010000000
VERSION     DADMGC010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.307"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(195..347)
                     /locus_tag="JNZ73_004305"
     CDS             complement(195..347)
                     /locus_tag="JNZ73_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734318.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            365..556
                     /locus_tag="JNZ73_004306"
     CDS             365..556
                     /locus_tag="JNZ73_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HAY8734319.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWLHH
                     QSKKEAQQSTLSVESPVQR"
     gene            867..1385
                     /locus_tag="JNZ73_004307"
     CDS             867..1385
                     /locus_tag="JNZ73_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY8734320.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
BASE COUNT          432 a          326 c          281 g          450 t
ORIGIN      
        1 cattcagttt catagatgct cagcagaatc ccccacatcc tgaaggaggt gtattcagac
       61 aggcatccca cctgacttcg aatgatgatt attcatcact atagagagca ttgattctaa
      121 gtgtcatatg aaagttccaa ttgatatata tcaaacaaaa taaccctgat taatgaatta
      181 ttacgtttat catgttaatt catcattatt acatcatcat tgtaaataat taaattaact
      241 tccataacat taaaatatgt atccactgac gcttttttac ataacgaaga attgaccatt
      301 ttgtcctgtt gtgccttaat gtaagtaccg tccacagcgt gggacatact tcaaggaacc
      361 ttttgtgagt caggcaacta gtatgcgaaa acgacaccga tttaacagtc gcatgacccg
      421 tatcgtactg ctcatcagct ttatcttctt ctttggccgt tttatctact cgtccgtcgg
      481 tgcctggctg caccatcaga gcaaaaaaga agctcagcaa tccacactct ccgtcgaatc
      541 accggtacaa cgttagcggt taccttctcc actttcacag aacataacgg cacttcgctg
      601 tcggatgctt ttgctctttg ggattatcaa agcggcagat attctttcat cttaaatttt
      661 acgtctttgt cctgactgat gtttatcctg tttggctgcg aaataaatat aaaattaata
      721 tatatgttgt aatgatatat ttttataaat tattccctgc gtgaatttta ataaatttaa
      781 tctatccctt tatacgcaat acatttactt tcctcttttg atgatcttaa atgtcttatt
      841 tttcgtaatg tgtataacaa ggaatagtga tgaaatttaa aaaatgtctt ctgcctgtgg
      901 caatgttagc gtcattcact ctggcaggat gccagtcaaa tgctgacgat catgccgccg
      961 atgtttatca aaccgatcaa ctgaatacca aacaagaaac taaaaccgtt aatattattt
     1021 ccattcttcc cgcaaaagtt gccgtagaca actcccaaaa taaacggaac gcacaagcct
     1081 tcggcgcgct tattggcgca gtcgctggcg gtgttatcgg ccacaacgtc ggttctggca
     1141 gcaattccgg aacgacggca ggggcagttg gcggcggagc tgtaggcgcg gcagcgggtt
     1201 ctatggtgaa tgataaaacc ttagtggaag gtgtttcttt aacatataag gaaggcacca
     1261 aagtgtatac ctccacccag gagggtaaag agtgccagtt tacgacaggt ttagccgttg
     1321 ttattaccac aacgtataac gaaacgcgta ttcagccaaa taccaaatgt cctgaaaaga
     1381 gctaataatc aggaggagtc atgaagaaag tttttctttg cgccatctta gcctccttaa
     1441 gctatccggc tatagcctca tcattgcagg atcaactctc tgctgtcgc
//
LOCUS       DADMGC010000302         1485 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.308, whole genome shotgun sequence.
ACCESSION   DADMGC010000302 DADMGC010000000
VERSION     DADMGC010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.308"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..1046)
                     /locus_tag="JNZ73_004308"
     CDS             complement(<1..1046)
                     /locus_tag="JNZ73_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HAY8734321.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          445 a          238 c          314 g          488 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggatacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtgt ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctattttat aatgactgtg tattcccatg gaaggagatc accca
//
LOCUS       DADMGC010000303         1432 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.309, whole genome shotgun sequence.
ACCESSION   DADMGC010000303 DADMGC010000000
VERSION     DADMGC010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.309"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..>1432)
                     /locus_tag="JNZ73_004309"
     CDS             complement(<1..>1432)
                     /locus_tag="JNZ73_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HAY8734322.1"
                     /translation="AIGLSIAAGGAALTWYQVAETDLSRTAAAAHEAVSVTERLLDEA
                     QGAIDLVYPLSVQPCTPQLRTELGRLTISHEHIRVINIFNQNLLSCSSWEGADPVREF
                     ISAGDNQTLILATDDYISPGVPVMILRKTDNDNAITASIATKWVADNMTLLNLRRPLS
                     LRVGNTVLTSDNTLVKAIPGGNRHAVYSGLYPFSIEYPGDLSVPLTLYFHDGALSLLL
                     SVLLGAAVATGLWKIKFRRKSRYDEIYEAMRKDEIVPWYQPIMDASTGEIAGVEVLAR
                     WVKADGEVIYPGSFITEVERSDLVIPLTRSLMARASRDLPPLTGDGKRWHISFNITQS
                     HIMETGFIAECLTFIDAFTPGSITLTIEPTEREPFDNSDEMRCQLKRIHDAGIALALD
                     DFGTGYANMEYLSEIPVDIIKIDRVFVNRIGQGESAEHLLTSLIEMANTLKKEIIAEG
                     VETEAQAAWLYERGVGKLQGFLYSPA"
BASE COUNT          366 a          381 c          336 g          349 t
ORIGIN      
        1 aatgccggtg aatataggaa tccttgtaac ttgccaacac cacgctcata tagccacgcg
       61 gcctgcgcct cggtttccac accttcggcg ataatctctt tcttcagcgt attggccatc
      121 tcgataaggc tggtcagcag gtgttctgca gattcgccct gacctattct gttcacaaag
      181 acgcgatcaa ttttgatgat atcgactggg atctcactaa gatattccat atttgcgtaa
      241 ccggtgccaa agtcgtccag cgcaagggca atgcctgcat catgaatacg cttaagctgg
      301 catcgcatct catcgctgtt atcaaagggt tctcgctctg tgggctctat agtgagagtg
      361 atactgcccg gtgtaaaagc atcaataaac gtcaggcact cggcaataaa tcccgtttcc
      421 ataatgtgtg actgcgtaat attgaaacta atatgccaac gctttccgtc gcctgtcaga
      481 ggcggtaaat cccgggatgc ccgcgccatc aggctacgag tcaatggaat aactaaatct
      541 gaacgttcta cttcagtgat aaaactgccg ggataaatga cttccccatc tgcttttacc
      601 caccgagcaa gtacttcaac gccagctatt tcacctgttg aggcatccat aattggctga
      661 taccacggca caatctcatc tttcctcatt gcctcgtaga tttcatcata gcggctctta
      721 cggcgaaatt ttattttcca caggccggtt gccacagcgg cccccagaag cacagaaagc
      781 agtaaagata acgcaccatc atgaaaataa agcgttagag gaacggaaag atcgccagga
      841 tattctatgg aaaagggata caggccagaa taaactgcgt gtctattccc tccaggtatc
      901 gctttcacca gcgtgttatc gctggtaaga acagtattcc ctacgcgcag cgacagcggc
      961 ctacgaagat tcagaagtgt catattgtca gccacccatt tagtggcgat actcgcagtt
     1021 atggcgttgt cattatccgt tttgcgtaga atcatgaccg gcacaccagg tgatatataa
     1081 tcgtctgtcg ccagaatcag cgtctggttg tcgccagcgg agatgaactc ccgcacagga
     1141 tctgcgccct cccaggacga gcaggacaaa agattctgat taaaaatgtt aatcacccgg
     1201 atatgttcgt gactaatggt caaccggcca agttcggttc tgagctgtgg ggtacatggt
     1261 tgcacactca ggggataaac cagatcgatt gcgccctggg cttcgtccag cagtcgttca
     1321 gtaaccgaga ccgcctcatg ggcagcagcg gctgttctgc tcaggtctgt ttcagcgacc
     1381 tgataccagg tcagcgcggc cccacccgca gcaatcgaaa ggccgatcgc ca
//
LOCUS       DADMGC010000304         1411 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.310, whole genome shotgun sequence.
ACCESSION   DADMGC010000304 DADMGC010000000
VERSION     DADMGC010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.310"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..680)
                     /locus_tag="JNZ73_004310"
     CDS             complement(<1..680)
                     /locus_tag="JNZ73_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8734323.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLT"
     gene            777..>1411
                     /locus_tag="JNZ73_004311"
     CDS             777..>1411
                     /locus_tag="JNZ73_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY8734324.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     KVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT          407 a          288 c          336 g          380 t
ORIGIN      
        1 ttggtcaacg aatatccatc atcaagaagc gtcactgcag aaataacgtg catttgctga
       61 acccagtggc gaaatgttaa tcccgtctct ttaacaaata agcgcatgag tgtacggcta
      121 ctgacgttaa ttttatccgc tagattatcc agtgttggca gatacccttg gttgtttagc
      181 aaattctcac aaagctgctg tagccgctca tcatttggcc aagggagttc aagtgacatt
      241 ttcaccccca tttttatttc ctgaagtatc agtgtctcga gggctgaaaa acgtaactgt
      301 tgtttatctg tggtactttg cgacggtgga agttggttaa ttgcaattag taattccctt
      361 aacaggggtg aaatacccgt catgaagcaa actgttccca gcgtttcact ttccgcttct
      421 gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa caaacttatg cggcatttgt
      481 ggcggcagcc aaagcgcgct gctggcatgg actagccaga cgctttctcc agcccataca
      541 cgaacgactc cttcagccgg gtaaagcaac tgggcataga gatgtttatg ccagggaact
      601 gtacctccat tcagatgttc cgaatccagt ccgataatgt gatgccctgg tgatgctttg
      661 ggttgcagtg taattgtcat ttacagtagg tattttgtca gaaatcatca aataagccat
      721 gttaaatttt aaatatgacg tatgcaagtt tccggatata tacggtgtaa agagtgatga
      781 taatttctaa aaaacagctt attggggtgg tagcgatcgg tattcttttg actggcgttg
      841 tcttttttat ctggagggta agcaaaggaa gatttataca gacgacggat gatgcttaca
      901 ttggtggtaa tatcaccacg gtggcaagta aagtttctgg ttatatttct gctatagagg
      961 tgcgcgataa tcagagcgta aaaaaaggcg atattatcct gcgtctcgat gatcgggatt
     1021 atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag tagcaaagcc aatctcgaag
     1081 gcattcaagc cactattacg atgcagcagt cgataattca aagtgccagt gaaacatggc
     1141 aggcagtaaa acatgaggag caaaagcgcc tgcgagatac tgaacgttat gaaaaactcg
     1201 cacaatcagc ggcaatatcg caacaaatta ttgataacgc caggtttgac taccagaagg
     1261 tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt ggagaagcaa cggctagcag
     1321 ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga agaagttcag gccgctttga
     1381 ctcaggcact actggatctg gaatataccc t
//
LOCUS       DADMGC010000305         1402 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.311, whole genome shotgun sequence.
ACCESSION   DADMGC010000305 DADMGC010000000
VERSION     DADMGC010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.311"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..178)
                     /locus_tag="JNZ73_004312"
     CDS             complement(<1..178)
                     /locus_tag="JNZ73_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY8734325.1"
                     /translation="MKSMDKMSTGIAYGTSAGSAGYWLLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFK"
     gene            complement(291..527)
                     /locus_tag="JNZ73_004313"
     CDS             complement(291..527)
                     /locus_tag="JNZ73_004313"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734326.1"
                     /translation="MAITSALQADDAGSIPAAAQVKVFQSAMMGKHGVTERRLRFECS
                     SFLQNCCAEIPTFGQAIIHKHSAFIPINCGWFVG"
     gene            complement(471..541)
                     /locus_tag="JNZ73_004314"
     tRNA            complement(471..541)
                     /locus_tag="JNZ73_004314"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(506..508),aa:Val,seq:tac)
     gene            complement(545..620)
                     /locus_tag="JNZ73_004315"
     tRNA            complement(545..620)
                     /locus_tag="JNZ73_004315"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(585..587),aa:Thr,seq:tgt)
     gene            complement(626..699)
                     /locus_tag="JNZ73_004316"
     tRNA            complement(626..699)
                     /locus_tag="JNZ73_004316"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(663..665),aa:Arg,seq:tct)
     gene            complement(701..777)
                     /locus_tag="JNZ73_004317"
     tRNA            complement(701..777)
                     /locus_tag="JNZ73_004317"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(741..743),aa:Ile,seq:cat)
     gene            complement(986..>1402)
                     /locus_tag="JNZ73_004318"
     CDS             complement(986..>1402)
                     /locus_tag="JNZ73_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY8734327.1"
                     /translation="RSGNMFNQLLASQKMTKTAVNEALRRLKKAGIDKPELEAFLREM
                     IEGKQKSWLTHCTDAEALKIDSVIGEVLADHPGLLNVLSQRYVGRGMSKRRMAELLNE
                     QYPEWALITCRRRVDQWLRVAEFILYSPMREAFDYA"
BASE COUNT          377 a          373 c          302 g          350 t
ORIGIN      
        1 ttttgaaata cagattcgtc agataagtca gaagccccag aagcagactt cccagcacac
       61 caatcgcagc ccactgtgac ggactgacct gatcaagcca ctgtaaaagc cagtagccgg
      121 cactgccggc ggaggtgccg taggcaatgc ccgttgacat tttgtccata gatttcataa
      181 cctcacctcc gcaaataacg gatggcgtag ctttacactg agagacgaaa aggatttgaa
      241 aaaaagcccg caaaagcggg cgcaacggaa tctacagtaa ggaaagcact ctatccaaca
      301 aaccacccac agttaatcgg aataaaagca gagtgcttat gaatgatcgc ctgcccgaag
      361 gttggtattt ctgcacagca attttgcaaa aaagacgagc attcaaaacg caaacgtctt
      421 tcagtcactc cgtgctttcc catcatcgca gactgaaaga ctttaacttg agcggcagcg
      481 ggaatcgaac ccgcatcatc agcctgtaag gctgaggtaa tagccattat acgatgcccg
      541 catatggtgc cgactaccgg aatcgaactg gtgacctgat gattacaagt cagttgctct
      601 gcctgctgag ctaagtcggc gctagcccac caccgagaac tcgaacctcg caccgtcaac
      661 ttagaaggtt gatgctctat ccggatgagc tagcggtggt tggtggccct tgctggactt
      721 gaaccagcga cctggcgatt atgagtcgct cgctctgacc aactgagcta aagggccgga
      781 ggcagaataa taaccatatg tcatcacatc tgcaaactca tctgaccacc agcacgttta
      841 acgtcctgtg ccatttttca ggcataaaaa aacccgctca atggcgggtt ctggtaaagt
      901 tcatgcgctt ggttcgcctc gcgatacagc tttgcgaagc tgtacctgaa ttgaagcagt
      961 ttgtggctca ttttgcaatg attttttaag cataatcgaa cgcttctctc atcggtgaat
     1021 acaaaatgaa ctcagcaaca cgcaaccact gatcaacacg acgtcggcat gtaatcaacg
     1081 cccactctgg gtactgttcg tttaataact cggccatcct tctcttactc atccctcgcc
     1141 ccacataacg ctgactcagg acatttagta gtcctggatg atccgccaga acttcaccta
     1201 taacactatc aatttttagt gcctctgcgt cagtgcagtg agttaaccag cttttttgct
     1261 ttccttcgat catctcacgc aaaaatgctt ccaactctgg tttatcaatt cccgcttttt
     1321 tcaatctgcg cagggcttca ttgacggctg tttttgtcat tttttgggat gccagcaact
     1381 ggttaaacat attccctgac ct
//
LOCUS       DADMGC010000306         1394 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.312, whole genome shotgun sequence.
ACCESSION   DADMGC010000306 DADMGC010000000
VERSION     DADMGC010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.312"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..170)
                     /locus_tag="JNZ73_004319"
     CDS             complement(<1..170)
                     /locus_tag="JNZ73_004319"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734328.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAEPADVLAARAEPAASVA
                     LVAASLADVPASL"
     gene            307..>1394
                     /locus_tag="JNZ73_004320"
     CDS             307..>1394
                     /locus_tag="JNZ73_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8734329.1"
                     /translation="MEDASTTKKGIVQLSSATNSASESLAATAKAVKVVMDETNKKAP
                     LNSPALTGTPTTPTAPKGTNNTQIASTAYVMAAIAALVDSSPDALNTLNELAAALGND
                     PNFATTMTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAK
                     STVAAVIEYLGLQETVNKADNAVQKTGDTLSGGLTFENDSILAWIRNTDWAKIGFKND
                     SDADTDSYMWFETGDNGNEYFKWRHRLAGGQLKELMNLKWDSLNILVNAVINGCLGIG
                     TTNALGGNSIAFGDNDTGLKQNGDGLLDVYANGQHVFRFQNGVAIAFKNIQAGTARKF
                     TLSSANNSTKNAAFYLWGNPSRPVVAELG"
BASE COUNT          365 a          343 c          398 g          288 t
ORIGIN      
        1 agggatgccg gcacatctgc cagtgaggcg gcaaccaatg cgactgatgc tgcaggctca
       61 gcacgcgcag ccagcacgtc agccggttcg gcagcgtcat cggcatcatc tgcgtctgct
      121 tcaaaagatg aggcgactaa acaggcgtca gcagcgaagg gcagtgccac gacggcatcc
      181 tctaaggcga cagagtctgc aggcagtgcg acggcggcag cgcagagcaa aagcgcggca
      241 gaatccgcgg caatgcgcgc tgagacagca gcaaaacggg cagaggatat tgctgatgtc
      301 gtatctctgg aagatgcgag cacgacgaaa aaggggatag tgcagctcag cagtgcgacc
      361 aacagtgcat ccgagtcact ggccgcgaca gcgaaagcgg ttaaggtggt aatggatgaa
      421 acaaacaaga aagcgcccct gaacagtccg gctctgaccg gaacgccaac cacacccact
      481 gcgccaaagg ggactaataa tactcagatc gccagtacgg cttatgttat ggccgcgatt
      541 gctgccctcg tggactcgtc gcctgatgca ctgaacacgc tgaatgaact ggcggcggcg
      601 ctgggcaacg acccgaattt tgcgaccacc atgactaacg cgcttgcggg taagcaaccg
      661 aaagatgcca ctctgacggc gctggcgggg cttgctactg cggcagacag gtttccgtat
      721 tttacgggga atgatgtcgc cagcctggca accctgacaa aagtcgggcg ggatattctt
      781 gcgaaatcga ccgttgccgc cgttatcgaa tacctcggtt tacaggaaac ggtaaacaag
      841 gctgataacg ctgtccaaaa gacaggcgat accttgtccg gtggacttac ttttgaaaac
      901 gactcaatcc ttgcctggat tcggaatact gactgggcga agattggatt taaaaatgat
      961 tcggatgcag acaccgattc ctacatgtgg tttgaaacag gtgacaacgg caatgagtat
     1021 ttcaaatgga gacaccgcct cgctggcggc cagctcaaag agctgatgaa tcttaaatgg
     1081 gattcactaa atattctggt taatgccgtc attaatggtt gccttggcat tggtacgacg
     1141 aatgcgttag gtggaaattc aattgctttc ggggataacg ataccggcct gaaacagaac
     1201 ggcgatggtc tgctggatgt ttatgcgaat ggacagcatg tatttcgttt tcagaatggc
     1261 gtggctattg cttttaagaa tattcaggcc ggaactgcca gaaaattcac gttatccagc
     1321 gccaacaact ccacgaaaaa tgcagcgttt tatttgtggg gtaatccatc caggcctgtt
     1381 gttgcagagc ttgg
//
LOCUS       DADMGC010000307         1389 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.313, whole genome shotgun sequence.
ACCESSION   DADMGC010000307 DADMGC010000000
VERSION     DADMGC010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.313"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(1..572)
                     /locus_tag="JNZ73_004321"
                     /pseudo
     CDS             complement(1..572)
                     /locus_tag="JNZ73_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(620..1114)
                     /locus_tag="JNZ73_004322"
     CDS             complement(620..1114)
                     /locus_tag="JNZ73_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8734330.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTGQAIAATGV
                     GIRIFNQSDNSQVKLYNDSDYTAIDAEGKAEMKFIARYVATNATVTAGTANADSQFTV
                     EYKK"
BASE COUNT          423 a          280 c          263 g          423 t
ORIGIN      
        1 atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc ttcccggcgc
       61 catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg tcgccagaac
      121 agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt gctcgctgat
      181 ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg caatgacaca
      241 cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg tgatactggc
      301 ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc tacccaagcc
      361 tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt atcagcatta
      421 ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt taatgctggt
      481 tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac tgttgtttta
      541 aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa gccaattgaa
      601 taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa tcagcgttcg
      661 ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac ttcatttcag
      721 ccttgccttc agcatcgata gcggtataat cagagtcgtt ataaagttta acctgcgaat
      781 tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct tgtcctgtac
      841 catcaaaacg aacggctgct tgagtgtacg ttgccgggca attttcaagt ttaatactga
      901 acttctgtgg cgacgccaga ctacctacac ctgaaaatgc atttttactg actttaccca
      961 gtgggacaat ttgatcggct gtatcggagg taacttcaca agtagtgtcg acaatttcac
     1021 cgacgaagtt aacaacacca tcttctgcca gtgcatgccc ggccagcatt agagatgcca
     1081 gagtgaatgc accgcctata atattacgct tcataaaaac tccattgata taatatatcc
     1141 tttaccatga atcatcactt atattaataa gtgtattttt gattcaaagg aatagtacac
     1201 caaagagacg gctttatcta aaaataatat taattacgca aaaataaaat atagatttat
     1261 tgtttaaata ttatttaaac agtaaaaaat caagtttgtt atcagacatt aatgataatt
     1321 cagaatatat ttatatagaa agatagatga atttattgat gtcagttata aaaaagggcc
     1381 accggtcgg
//
LOCUS       DADMGC010000308         1355 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.314, whole genome shotgun sequence.
ACCESSION   DADMGC010000308 DADMGC010000000
VERSION     DADMGC010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.314"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            193..462
                     /locus_tag="JNZ73_004323"
     CDS             193..462
                     /locus_tag="JNZ73_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734331.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            714..1304
                     /gene="ospG"
                     /locus_tag="JNZ73_004324"
     CDS             714..1304
                     /gene="ospG"
                     /locus_tag="JNZ73_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HAY8734332.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          389 a          262 c          333 g          371 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgaccaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattttgtgt gaaaatctct tgtgattttt aaatatcgta acaaaggtat gacatgaaaa
      721 taacatctac cattattcaa acaccttttc catttgagaa taataattct catgctggca
      781 tagtaacgga gcccattctc ggtaagttaa taggtcaggg gtcgacagca gaaatctttg
      841 aagatgtgaa tgattcatct gctttgtata aaaagtatga tcttattggc aaccagtaca
      901 atgagattct ggaaatggct tggcaagaat ctgagctttt taatgctttt tatggcgatg
      961 aagcatccgt tgttatacag tatggcggag atgtgtacct ccgaatgctg cgcgtgcctg
     1021 ggactcccct tagtgacatt gatacagctg atatccctga taatatagag agcctttatc
     1081 tacagttgat atgtaaattg aatgagttga gtataatcca ttacgatctt aatacaggta
     1141 atatgctgta tgataaagaa agtgaaagtt tattcccaat agattttcgc aatatttatg
     1201 ctgaatatta cgctgcaacc aaaaaagata aagagattat cgaccgacga ttacaaatgc
     1261 gtacaaatga tttttattcg ttattaaaca ggaaatattt atagccgtat ttgttgatgc
     1321 tataagccag aggctcttct atgagaatat gggga
//
LOCUS       DADMGC010000309         1352 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.315, whole genome shotgun sequence.
ACCESSION   DADMGC010000309 DADMGC010000000
VERSION     DADMGC010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.315"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            144..758
                     /locus_tag="JNZ73_004325"
     CDS             144..758
                     /locus_tag="JNZ73_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HAY8734333.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            758..1087
                     /locus_tag="JNZ73_004326"
     CDS             758..1087
                     /locus_tag="JNZ73_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HAY8734334.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            1099..>1352
                     /gene="ubiA"
                     /locus_tag="JNZ73_004327"
     CDS             1099..>1352
                     /gene="ubiA"
                     /locus_tag="JNZ73_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HAY8734335.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAV"
BASE COUNT          298 a          325 c          371 g          358 t
ORIGIN      
        1 ggcgattgtt ggcttcgcag gcatgatcat cacctggatc gtgaaaagct tcgacgagga
       61 cgtggattac tacgtgccgg tggcagaaat cgaaaaactg gaaaaccagc atttcgatga
      121 gattactaag gcagggctga aaaatggcaa ctgatacttt gacgcacgcg actgcccacg
      181 cgcacgaaca cgggcaccac gatgcaggcg gaaccaaaat cttcggattt tggatctacc
      241 tgatgagcga ctgcattctg ttctcaatct tgtttgctac ctatgccgtt ctggtgaacg
      301 gcaccgcagg cggcccgaca ggtaaggaca ttttcgaact gccgttcgtt ctggttgaaa
      361 ctttcttgct gttgttcagc tccatcacct acggcatggc ggctatcgcc atgtacaaaa
      421 acaacaaaag ccaggttatc tcctggctgg cgttgacctg gttgtttggt gccggattta
      481 tcgggatgga aatctatgaa ttccatcacc tgattgttaa cggcatgggt ccggatcgca
      541 gcggcttcct gtcagcgttc tttgcgctgg tcggcacgca cggtctgcac gtcacttctg
      601 gtcttatctg gatggcggtg ctgatggtgc aaatcgcccg tcgcggcctg accagcacta
      661 accgtacccg catcatgtgc ctgagcctgt tctggcactt cctggatgtg gtttggatct
      721 gtgtgttcac tgttgtttat ctgatggggg cgatgtaatg agtcattcta ccgatcacag
      781 cggcgcgtcc catggcagcg taaaaaccta catgacaggc tttatcctgt cgatcattct
      841 gacggtgatt ccgttctgga tggtgatgac aggggctgcc tctccggccg taattctggg
      901 aacaatcctg gcaatggcag tggtacaggt tctggtgcat ctggtgtgct tcctgcacat
      961 gaataccaaa tcagatgaag gctggaacat gacggcgttt gtcttcaccg tgctaatcat
     1021 cgctatcctg gttgtaggct ccatctggat tatgtggaac ctcaactaca acatgatgat
     1081 gcactaagag cggcggttat gatgtttaag caatacctgc aagtaacgaa accaggcatc
     1141 atctttggca acctgatctc ggtgattggg ggattcctgc tggcctcaaa gggcagcatt
     1201 gattatcccc tgtttatcta cacgctggtt ggggtgtcac tggttgtggc gtcgggttgt
     1261 gtgtttaaca actacatcga cagggatatc gacagaaaga tggaaaggac gaagaatcgg
     1321 gtgctggtta aaggcctgat ctctcctgct gt
//
LOCUS       DADMGC010000310         1345 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.316, whole genome shotgun sequence.
ACCESSION   DADMGC010000310 DADMGC010000000
VERSION     DADMGC010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.316"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            443..1192
                     /locus_tag="JNZ73_004328"
     CDS             443..1192
                     /locus_tag="JNZ73_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY8734336.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          376 a          274 c          279 g          416 t
ORIGIN      
        1 gagtggtgga ttagcccggc aggtgggcgc tggctcctga gcaccataaa cagggcttta
       61 cccgtaaata ttggctcctt ctgatgccat tctatttcag gaaggagtgt ccgttaaact
      121 caggctacct caaaaagtta tcggtgcgtt tatcgaaaaa catatgttct actaattgga
      181 gtcattgtct gtaaaagcaa ctcaaaaata caagttgctt attccccttg ttggtaagaa
      241 cttaattcta actgtttgtt ctggatgttt taaacgtcct cctttttatg cttgttcata
      301 acggatattt aataggtaac ctttgtttta gcatcttctg atttatttaa attatgtgat
      361 agcgcacaca attttattat ccagtacttt atactcttaa taaaataatt ataaatattt
      421 atcctctata aggagatgtt atatgaaaag acagcttttt actcttagta ttgtcggggt
      481 attttcttta aatacctttg catcattccc tccgggaaat gatgtgacaa caaaacctga
      541 cctttactac ctgacaaacg ataatgctat tgacagtctg gcattattac cgcctccacc
      601 acaaatcgga agtattgcct tcctgaatga ccaggccatg tatgaaaagg ggcgtttatt
      661 gcgaaatact gaaagaggga agctggcagc tgaggatgcc aatctcagta gtggtggcgt
      721 ggcaaatgta ttttctgctg cttttggttc tccgataacg gctaaagatt caccagaatt
      781 gcataaatta ctgacaaata tgattgagga tgccggagac ctggcaacgc gttcagcaaa
      841 agaatattat atgcgtattc gaccttttgc tttttatggt gtttctacgt gtaatacaaa
      901 agaacaggat acattatcca gaaatggctc ttatccatca ggtcatacat cgattggttg
      961 ggcaacagca cttgtattgt ccgaaataaa tccggcacgc caggatacca ttctgaaacg
     1021 gggttatgaa ctgggggaca gcagggttat ttgcggttat cactggcaaa gtgatgttga
     1081 cgcagcacgt attgttggct ctgctattgt agcaacactg cactcaaatc ctgtgttcca
     1141 ggcacaatta caaaaagcga aagatgaatt cgctaacaat cagaaaaaat aatgctttta
     1201 gccagtacgc cttataaccc cgaccttcag ggctaatgcc agtcagttaa gcaactgact
     1261 ggctcttttt cagggctgtg gggtatttcc agggcctctc ctttaccact ctcgggaagg
     1321 ccctttccct tcttgtcggt aattt
//
LOCUS       DADMGC010000311         1313 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.317, whole genome shotgun sequence.
ACCESSION   DADMGC010000311 DADMGC010000000
VERSION     DADMGC010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.317"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..845)
                     /gene="ipaH4.5"
                     /locus_tag="JNZ73_004329"
     CDS             complement(<1..845)
                     /gene="ipaH4.5"
                     /locus_tag="JNZ73_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HAY8734337.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPIFTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GPQIY"
BASE COUNT          372 a          236 c          292 g          413 t
ORIGIN      
        1 aagtaaatct gcgggccgtg gtagtccggc gaagaggtta atctttgcag ggattgcaga
       61 acgtgagtcg acaatggatt ccctgaaatg ttaacattcg taaaaatggg caatgtgatt
      121 atattttcgg gtaatgaagt tagtaggtta ttggaaacat ccagtgccaa tagttcttgc
      181 ggtaatctgg ataatctgcg tagttgattt tcacctacct tcaaatattt taagctctga
      241 ggaaattctg gtagttcctg tagtctgttg ccggaaacaa agagttcttg tagtctatga
      301 ggcaattcag gcaagacagt aaggcgatta ccttcaatac gtaatattgt tagcgttgat
      361 ggaagctctg gtaacgtctc cagactatta aaacgtgccg agagtgattg taaatatggt
      421 ggtaatgatg gcagacatga caaatttcga ttgaatgaaa tattaagctt aatcagttga
      481 gatggaagtg tcgggagtat attcaagttg ttaccattta catgaagttc tgtcaaaagc
      541 ggagaatttt caggtaggga gattaactca ttgttggaaa tattaagttc acgtatatgc
      601 aaagggagtg gtggtaaaga acgtagtttc agtaagctta aattgagaac aggctcccgg
      661 gtatccagac atattttcaa taatcgaacc gcctggatgc gattttctgt tgttgttcct
      721 tccctagccc agtcatccca gatgcggtgg taatctctgg tacactgttc ttctaccgat
      781 aaacgagata tacatgataa gccacaaagg gaacgaaaaa aagaatgatt gttgatcggt
      841 ttcatttatt aacccctcac tattcatatc ttagtctatt aatgcaaata aaacgagaaa
      901 gtctttaaaa aaacaatcct agagctttat cggcgttttc attccggctg gaggaatatg
      961 ttcctgtctt tccagtaacc gtcgattgtt atatcagtct acccatgtta atgtgaccag
     1021 ttctactttc atccagtttg gattgatctt tatcccctat attttcaaac aatttttatc
     1081 atttaactca ttactgtctc gatattccca tagcaaacga tctggttggt tatcccctca
     1141 aaaaagtcag ttgcacgccg taaatccctg ccggtattga gcgaggattt acgggttgtt
     1201 gttaaggtcc gtaatcgggt cacagttttt ccggagtcaa tccggtctgc ggtttatgct
     1261 tatgcgacgt gattatgaat ggtgcagtcg tgagccgttt tcagacaatc gca
//
LOCUS       DADMGC010000312         1298 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.318, whole genome shotgun sequence.
ACCESSION   DADMGC010000312 DADMGC010000000
VERSION     DADMGC010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.318"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..332)
                     /locus_tag="JNZ73_004330"
     CDS             complement(<1..332)
                     /locus_tag="JNZ73_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY8734338.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFS"
     gene            complement(398..1117)
                     /locus_tag="JNZ73_004331"
     CDS             complement(398..1117)
                     /locus_tag="JNZ73_004331"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAY8734339.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
BASE COUNT          396 a          304 c          262 g          336 t
ORIGIN      
        1 gaaaaaacaa taaacccagt accggccata atggtcatag accaggtgat aatcggcgcg
       61 attatgttga tatatttcgg gttacgccgc ataaaattac cagcgacgcg taccagataa
      121 tccattccgc ctgcggcctg taaggctgag gccgccacaa caacggtcat aataatcagg
      181 atcacgtcga ctggcggcga ccccataggc agaccaaatc ccagagtcag aatggcaagc
      241 ccaagcccgc cacataaacc aataccgatt cccccctggc gaataccaaa aaatattgca
      301 ccaagtacta cgataatttc cagccaaacc ataatgttct ccgttataaa tataattcag
      361 ttaaaagcga ataactttct ggctgcgatt taaatgatta ttgggttaaa agataatgtt
      421 taccgacacg attttcgtac cattttattc cggcacgaac gagtgacgcc gttgagtcaa
      481 tatagcggga aggctgctct ttaacggctt gcgccagaat aaccggaatt tctgtacaac
      541 caagaacaag gatttctgca ccgcgggaaa ataatatttc agcctgctca ttcatcaact
      601 tttgtgcgtg cgctatattg ccagctttca aggaataaat actttccatc acttttttct
      661 gaccgtcggc atcggggctg acacaggtaa atcccagcga ctcaattcct ttttggtata
      721 aacccatata tagcgtggca ttggtggcta acaagccaat tcgggtttta ccacaggcgc
      781 gaacttcatt gatcgtagtt tctacgatac ttaatatatc aatatgacaa gcatctttta
      841 attctttgaa ccagtaatgc gcggtattac agggaataac aatacattct gccccggcat
      901 cttccagctt atgcatataa tcacgcataa cgggtaatgg tgaacgccca tgatgcatta
      961 atgcatcggt acggtcagga atatccggga tcgaagaaat aattaaagga atatgttcct
     1021 gatcacattg tgcagcggta aaagttacaa atttattaaa caggtcgacg gtagctgccg
     1081 gccccattcc accgagtaca ccaattaaac ctttcacaat acctgcctct caaaacgtta
     1141 atgacaatac gcggtaaata actgctgctc tcttttttaa cagtcgaaat gggcgatgag
     1201 aaatgcaagg ttgtacaagg cgatgcaaga cacgcatgat gcagctatgg aacattccgc
     1261 ttattcacca tagtgatgat gtattctggt tatctgac
//
LOCUS       DADMGC010000313         1297 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.319, whole genome shotgun sequence.
ACCESSION   DADMGC010000313 DADMGC010000000
VERSION     DADMGC010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.319"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..123)
                     /locus_tag="JNZ73_004332"
     CDS             complement(<1..123)
                     /locus_tag="JNZ73_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8734340.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYA"
     gene            181..1206
                     /locus_tag="JNZ73_004333"
                     /pseudo
     CDS             181..1206
                     /locus_tag="JNZ73_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            1179..>1297
                     /locus_tag="JNZ73_004334"
     CDS             1179..>1297
                     /locus_tag="JNZ73_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HAY8734341.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQV"
BASE COUNT          361 a          316 c          301 g          319 t
ORIGIN      
        1 ggcataaagg ctgtgggtgg tgatatcgag acgcgttgca acgctggcaa cagaataacc
       61 gcgatcaaca acctgtttga ctgcttcagt tttaaactct tcgggataac gcttaccgct
      121 catgggcacc tctctttaag ccatcttaaa tgactctgag gtgtctgtta aacccgtggc
      181 gattcacaat tctcttgcac cattaactat gttcagtccc tgcgcgagtt gtacaccgat
      241 gtcatcgaag agttttactg ggttgcgctc ccgcttacga cgcaaaattc cctttcacaa
      301 taccaacccg aatggcagtg ctgagaacct gatgtcgaat gggtgcgtca gcccccgcaa
      361 gatgcgataa ccgctcctga ctttttctgc ttttaccagc caggcatgac cttcgaacaa
      421 tttgtacgtg agtttgccga atggttttca caaaaacgtc cggcggcgat gatgatcggc
      481 atccgtgcgg atgagtccta caaccgtttt gtcgccatcg ccagtttaaa taaacaacgt
      541 tttgccgacg ataaaccatg gactacggcc gcaccaggcg gtcatagctg gtacatttac
      601 cccatttacg actggaaagt ggctgatatc tggacctgat atgcaaatca tcaaaatctc
      661 tgcaacccac tgtataacct aatgtatcag gcaggcgttc ctctgcgtca tatgcgaatt
      721 tgcgaacctt ttggcccgga gcaacgacaa ggattatggc tctatcacgt tatcgaaccg
      781 gatcgctggg ctgctatgtg cgcacgagtc agcggggtaa aaagtggcgg catttacgcc
      841 ggacatgaca atcatttcta tgggcatcgg aaaatcctca aaccagaaca tttagactgg
      901 caagaatatg cattattgct gctcaatagc atgccggaaa aaacagctga gcattaccgc
      961 aataaaatcg ccatttattt gcactggtat cagaaaaaag gcatcgagat tccacaaacc
     1021 cagcaagggg acattggcgc gaaagatatt ccctcctggc ggcggatatg caaagtttta
     1081 ctcaataacg attactggtg tcgggcatta tcatttagtc ctacgaaagc gaagaactat
     1141 cagcgttata acgaacggat aaaaggaaaa cgtcaggaat gggggatact atgcaacaac
     1201 gattaacgca ggatttaacg caatttctcg ccagcttgcc ggaagatgat cgcatcaaag
     1261 cgattaatga aatccgcatg gcgatccatc aggttag
//
LOCUS       DADMGC010000314         1296 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.320, whole genome shotgun sequence.
ACCESSION   DADMGC010000314 DADMGC010000000
VERSION     DADMGC010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.320"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..815)
                     /locus_tag="JNZ73_004335"
     CDS             complement(<1..815)
                     /locus_tag="JNZ73_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY8734342.1"
                     /translation="MKPVHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="JNZ73_004336"
                     /pseudo
     CDS             907..993
                     /locus_tag="JNZ73_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1003..1083)
                     /locus_tag="JNZ73_004337"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="JNZ73_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          361 a          218 c          338 g          379 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gactaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtggacaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tgtgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctt
//
LOCUS       DADMGC010000315         1263 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.322, whole genome shotgun sequence.
ACCESSION   DADMGC010000315 DADMGC010000000
VERSION     DADMGC010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.322"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            66..1259
                     /locus_tag="JNZ73_004338"
     CDS             66..1259
                     /locus_tag="JNZ73_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HAY8734343.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
BASE COUNT          377 a          265 c          289 g          332 t
ORIGIN      
        1 aacgagacaa acatagcatt atgtgatcta ctgatttgtg ccaacaattc aatgagatca
       61 ctgctatgcc tgttcataaa gtatgtcaga atttcttccg ggatgcctta gcaccatttc
      121 atcagtaccg acaaaatgcc ctgatggatg ccacaatggc gttaatcaac ggagcatctc
      181 tgacgcagac cagtatcgga cgttttttgc cgggtaatgc acaagttaag aataagatca
      241 aacgtattga tcgcctcatg ggtaatgagg cgctccatcg cgatattcca atgattttca
      301 ggaatatcac ttcaatgctg acacggcagt tatctttatg tgtaatagcc gtagactgga
      361 gtggctatcc ctcccaggag catcatgttc ttcgtgccag ccttctgtgt gacgggcgtt
      421 ccattccgtt attgagtaag gttgttcctt ctgaaaaaca aaataatcca ttgatacaac
      481 atgatttcct tgattccctt gctcaatccc tgccccccga tgccagggtt attattgtca
      541 cggatgctgg atttcagagc gcatggtttc accatattac ttctctgggc tgggatttta
      601 ttggtcggat cagaaataac gttcagtatt gcctcgataa cgcgcctgaa agatggttaa
      661 aagtgtcgga tagccctgaa tgtaaaacac cggaatatat gggggctgga aggctggtta
      721 aagaaaggaa aaaaagcatc cgggggcatt tttacaccta taagaaatcg gcaaaagggc
      781 gaaaaaagaa acgttcgaaa ggtcagagtg gtttaaacaa aacagacaaa gaacagagca
      841 aatcagcaaa agaagcctgg ttgatcttca gcagtacaaa tgacttcaga gcgcgagaaa
      901 tcatcaagtt atacagtcgg cgaatgcaga tagagcaaaa ctttcgtgat gaaaagaacg
      961 ggcgcttcgg atttggtctt cgggccagca aaagtcgttc aacaggaaga attctggttc
     1021 tgagtctgct ggcaacgttg agcactatcg taatgtggtt actcggttat cacgctgaaa
     1081 ataaagggtt acacctgaaa tatcaggcta acagcattaa atcccggcgg gttatctcat
     1141 atctgacgtt agcgaagaat gttcttcgac actcgccact tattttaaga cgaacagtac
     1201 tgagcacagt tctaaaccat ctgtccagaa cctaccgaaa tatggtgctg gtttattagc
     1261 agt
//
LOCUS       DADMGC010000316         1205 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.323, whole genome shotgun sequence.
ACCESSION   DADMGC010000316 DADMGC010000000
VERSION     DADMGC010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.323"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            14..925
                     /locus_tag="JNZ73_004339"
     CDS             14..925
                     /locus_tag="JNZ73_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY8734344.1"
                     /translation="MINEGDKSQEDIADEAGMSPATLSALRKGEYNGNVEGMHDKLQK
                     WYQVWQKGQNLSGTPGVVKTQIFCDLHELFKTVRSLGIISVVVGVPGVGKTFAAREYC
                     RRTANAWMVTLAPAHSSVTECLLEIAYALEINNPGRNKGNITRAIRRKLGGGKETSGR
                     VNPLLIIDEADHLSVDGLEQLRAIQDATGVGMVLIGNPKQMADATRRGTDELARLFSR
                     FAMTKQLRKVKKTDVMAVAKAWGVTQEDELDLLMRVAEKPGGLRVLTHTLNHAWLAAK
                     GAGTSLNKSHIKAAFKEAYSNPRLLNW"
     gene            941..1168
                     /locus_tag="JNZ73_004340"
     CDS             941..1168
                     /locus_tag="JNZ73_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734345.1"
                     /translation="MMSRNIKMATDAQNWLQAHGSHVNESYLGVARPVLEITCPPPEL
                     VRNAVRIMEHKSGVARSVWTARLNGCQIIWR"
BASE COUNT          338 a          256 c          336 g          275 t
ORIGIN      
        1 cactattaac gctttgatta acgagggcga caagtctcag gaagatattg ctgatgaagc
       61 cggaatgtct ccggcaacac tctcggctct gcgtaaagga gagtacaacg gtaatgtcga
      121 aggtatgcat gacaaattac agaaatggta tcaggtctgg cagaagggac agaacttgtc
      181 aggtacccct ggtgttgtga agacccagat attttgtgat ttgcatgagc tgtttaaaac
      241 agtgcgctca ctcgggatta tcagtgtcgt tgttggcgta ccgggtgtcg gaaaaacctt
      301 tgctgcccgg gaatattgtc gcaggacagc taatgcctgg atggtgactc ttgctccggc
      361 acattcgagc gtaacggaat gcctccttga aatagcatat gcgctggaaa ttaataatcc
      421 gggaagaaat aaggggaata taacccgcgc cattcgcaga aaactgggtg gtggtaaaga
      481 aaccagtggc cgtgttaacc cccttctgat tattgatgaa gctgatcact taagtgtgga
      541 tggtctggag caattacgcg ccattcagga tgccactggc gtggggatgg ttttaattgg
      601 caacccgaaa cagatggcgg atgccacccg tcgtgggact gatgaactgg cccgtctgtt
      661 cagccgtttt gccatgacaa aacaactgcg caaagtaaaa aagaccgatg tgatggctgt
      721 agccaaagca tggggagtaa cacaggaaga tgagctggac ctgctgatga gggtagccga
      781 aaagcccggg ggattgcgtg tattaactca cacactgaat catgcgtggc tggcggcaaa
      841 aggtgcaggc acatccctga ataaaagtca cattaaagcg gcttttaagg aggcttacag
      901 caaccccagg ttactgaact ggtagtaaag gacataaacc atgatgtcac gaaatataaa
      961 aatggcaacg gatgcgcaga actggttaca ggcgcacggg agtcacgtaa atgaatcata
     1021 tctcggcgtg gcgcgtccgg tacttgaaat cacctgccca ccaccggagc tggtcagaaa
     1081 cgctgtcagg attatggagc ataaatcagg ggttgcccgt tcagtatgga cggcccgcct
     1141 taatggctgc cagattatct ggcgttaata acggcttatt tatttgtgag gaataacaaa
     1201 atggc
//
LOCUS       DADMGC010000317         1200 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.324, whole genome shotgun sequence.
ACCESSION   DADMGC010000317 DADMGC010000000
VERSION     DADMGC010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.324"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..>1200
                     /locus_tag="JNZ73_004341"
     rRNA            <1..>1200
                     /locus_tag="JNZ73_004341"
                     /product="23S ribosomal RNA"
BASE COUNT          321 a          263 c          371 g          245 t
ORIGIN      
        1 accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga aggcccagac
       61 agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag ctcactggtc
      121 gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct gcggcagcga
      181 cgcttatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt actgtgaggt
      241 atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg ggtgaaaagc
      301 ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg tgagtcgacc
      361 cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc tgtacttggt
      421 gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg tcccggttta
      481 agcgtgtagg ctggttttcc aggcaaatcc ggaaaatcaa ggccgaggcg tgatgacgag
      541 gcactacggt gctgaagcaa caaatgccct gcttccagga aaagcctcta agcatcaggt
      601 aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc aaggcgcttg
      661 agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag aaggcacgct
      721 gatacgtagg tgaagcgact tgctcgtgga gctgaaatca gtcgaagata ccagctggct
      781 gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta tacggtgtga
      841 cgcctgcccg gtgccggaag gttaattgat ggggttagcg caagcgaagc tcttgatcga
      901 agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat tccttgtcgg
      961 gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac ccgagactca
     1021 gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa agaccccgtg
     1081 aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata ggtgggaggc
     1141 tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc tttaatgttt
//
LOCUS       DADMGC010000318         1189 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.325, whole genome shotgun sequence.
ACCESSION   DADMGC010000318 DADMGC010000000
VERSION     DADMGC010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.325"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..133)
                     /locus_tag="JNZ73_004342"
     CDS             complement(<1..133)
                     /locus_tag="JNZ73_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8734346.1"
                     /translation="MYNNKIDNNRKTRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     "
     gene            979..1176
                     /locus_tag="JNZ73_004343"
     CDS             979..1176
                     /locus_tag="JNZ73_004343"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734347.1"
                     /translation="MLGDNPNIDVVAMVPIIIVDMKIEVTLKLSDITVGNIMSAVSIM
                     AKDTLNTESVEKRLLSEKCMA"
BASE COUNT          383 a          218 c          229 g          359 t
ORIGIN      
        1 aaacaaaagg atacaatttg ccagccccca aaccaaggtg acctaaatat atagcaaaaa
       61 taccgagaaa ttttaaagca tcaacccaat catgccttgt ttttctgttg ttgtcgattt
      121 tgttattata catttaaagt aggctctcca gttttgtgtg attttcaaac actcattgca
      181 aatatgaatg cctgaaagtt tatgattatt aatcaacgag ttcaatatta atttattatt
      241 tttcaaagtc gcactatata ggcatttact tccgcagtac caccgatagt atcgatatca
      301 aatcagcctg cggccgcgat aactttcgta acatcctgcc agcggctttg ttgaataaat
      361 caggttcaag acaatcgtct ctgcatcagg ttagagtctc aggcaataca cactctttct
      421 ggcatagctg ccactctcgc accatggcca tagcctttac gatctgcata ctcaccagac
      481 cagagcccgc tccctttcgg ggaggaatga gggctctgat attcttttgc cgtagttcat
      541 cgtgaaaccg tcggatgtca tttggcctga actaccagtg gtgtaggcca ttcaatatcg
      601 tattatattg aaatataaat atgcatcagc tgcacacggt atatttccat gccagaagaa
      661 gtatcctgac gtgaaacatg actttgttgt caggatttca tgagagcggt tcagcaaaaa
      721 atgacgaaaa tcctagtata gtcgggtgat gagcagtata gaggaatgtg ttataaccaa
      781 taaaagtcca attaaatttg tttttatttc taagcccccc ccaaaaaaag gaggtggctt
      841 ttaaatttaa ttcaaatttt ataatggctc ttggcaaaat ttgctactat gttatttatt
      901 gcgattgggg ttgaaacaac aattgctgtt ataagaagca aatatggaat aaaataaatt
      961 ctattaccag aagcatatat gcttggtgat aatcctaaca tagatgttgt ggcaatggtt
     1021 ccaataatca ttgtggatat gaaaattgaa gtgacattaa aattaagtga tattacggtt
     1081 ggaaatataa tgagtgctgt aagtatcatg gcaaaagaca ccctaaacac ggagagtgtc
     1141 gagaaaagat tgctttcaga aaaatgtatg gcttgaaaaa taactggcg
//
LOCUS       DADMGC010000319         1169 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.326, whole genome shotgun sequence.
ACCESSION   DADMGC010000319 DADMGC010000000
VERSION     DADMGC010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.326"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(67..192)
                     /locus_tag="JNZ73_004344"
                     /pseudo
     CDS             complement(67..192)
                     /locus_tag="JNZ73_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(264..1148)
                     /locus_tag="JNZ73_004345"
     CDS             complement(264..1148)
                     /locus_tag="JNZ73_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HAY8734348.1"
                     /translation="MVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDD
                     PFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETT
                     DLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLWQDEHLL
                     RLCLRQALQLADVVKISEEEWRLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVC
                     YRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGAL
                     AVTAKGAMTALPCRQELE"
BASE COUNT          306 a          336 c          245 g          282 t
ORIGIN      
        1 acctgtgttg ttctgaggtg agcatatcac ctctgttcag gtggccaaat tcagtgtgcc
       61 acttcaccct agatgtcctt tcagccaaat agcgtataac gaccaccaca gcgaccagga
      121 aataaaaaag agaaatgagt aactggatgc aaaacgatag tacgcatttc tgaatggaat
      181 attcagtgcc ataattacct gcctgtcgtt aaaaaattca cgtcctattt agagataaga
      241 gcgacttcgc cgtttacttc tcactattcc agttcttgtc gacatggcag cgctgtcatt
      301 gcccctttcg ccgttactgc aagcgctccg caacgttgag cgagatcgat aattcgtcgc
      361 atttctctct catctgtaga taatcccgta gaggacagac ctgtgagtaa cccggcaacg
      421 aacgcatctc ccgcccccgt gctatcgaca caattcacag acattccagc aaaatggtga
      481 acttgtcctc gataacagac caccacccct tctgcacctt tagtcaccaa cagcatggcg
      541 atctcatact cttttgccag ggcgcatata tcctgatcgt tctgtgtttt tccactgata
      601 agtcgccatt cttcttccga gatcttgacg acatccgcca gttgtagcgc ctgccgcaaa
      661 cacaagcgga gcaaatgctc gtcttgccat agatcttcac gaatattagg atcgaagctg
      721 acaaaacctc cggcatgccg gatcgccgtc atcgcagtaa atgcgctggt acgcgaaggc
      781 tcggcagaca acgcaattga acagagatgt aaccattcgc catgtcgcca gcagggcaag
      841 tctgtcgtct ctaaaaaaag atcggcactg gggcggacca taaacgtaaa tgaacgttcc
      901 ccttgatcgt tcagatcgac aagcaccgtg gatgtccggt gccattcatc ttgcttcaga
      961 tacgtgatat cgactccctc agttagcagc gttctttgca ttaacgcccc aaaaggatca
     1021 tcaccgaccc gacctataaa cccacttatt ccgcctaatc tggcgattcc caccgcaacg
     1081 ttagctggcg cgccgccagg acaaggcagt aggcgcccgt ctgattctgg caagagatct
     1141 acgaccacat cccctaaaac ccatacttt
//
LOCUS       DADMGC010000320         1165 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.327, whole genome shotgun sequence.
ACCESSION   DADMGC010000320 DADMGC010000000
VERSION     DADMGC010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.327"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
BASE COUNT          318 a          210 c          225 g          412 t
ORIGIN      
        1 gtacgcgcta tcggaatcat tgaacctgtg agggccgccc cctttattac tcaggggggc
       61 tttcaacgtt ctgaattaag atgttttatg actcactgag caggatgtga gttccaacgt
      121 aatctccgct atttttactg tttttcattt gctgcctcat ctatcccttc gccttgcctc
      181 tgagttgcgt taaaggctga tgtactgcct ttaacccgtt caatacatag cctttttgtc
      241 aatcatcatc ccgaagagcg ttgcactgtg ccttccatag ctgtggttgt cctctcaatc
      301 tgtcagccca tttatggaaa atcttaacga agcaacaatg agatatgtaa aaaaagggcc
      361 gatttaatca ttcattaaga caggtcattt tttcttactg tgagggcggt aagcttttct
      421 gtcggtaaca ggttgggaga tgctggtatt atgagatttt tctaccgtcc tgcggcggaa
      481 tatgtgaaaa atagaaatga ttattattat tgcatggtta tttttagttt gtgatggtgg
      541 agaataatcc tattcccgtg accttgtttt ttacatttta tttaactgca tggtatatgc
      601 attttggacg gtgaaagcaa gaagctatta ggcgctatag gtcttttgac ggtttttgaa
      661 tttcccctcc tgatatctct gttatatttt tgtgaatttc tccaatccaa atcaaagtcc
      721 caataagtca tggatgcatg gttaaaataa ctatcatttg tgtaaccttt ggtgcttata
      781 acttttcttt attctttttt caggattacc tggagggatt gaccaaaaaa acattcattg
      841 aatgatattt ttctaattaa tgaggttttc taagctcgtt gaaataaaaa atatatttta
      901 atcatggatt tgcatgcgtt tctttcgctt tttttacaca agttgacatt gaactcagca
      961 actataagaa tagccacatc ccagacggtg accgataaag gaaataccgt taccatattt
     1021 aaatgaattg gaaaatatca ccgatttgaa gtcgactcgt tcgggtaatt attaatactg
     1081 aatctttaat gcttatttct taagcttgtt aacaacggga atgaataatg aaagtgtcca
     1141 aaattgcact ggcaatgggt cttag
//
LOCUS       DADMGC010000321         1155 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.328, whole genome shotgun sequence.
ACCESSION   DADMGC010000321 DADMGC010000000
VERSION     DADMGC010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.328"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            2..385
                     /locus_tag="JNZ73_004346"
     CDS             2..385
                     /locus_tag="JNZ73_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8734349.1"
                     /translation="MLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAFI
                     AVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLAL
                     NVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(386..1030)
                     /locus_tag="JNZ73_004347"
     CDS             complement(386..1030)
                     /locus_tag="JNZ73_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8734350.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAATPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          296 a          262 c          277 g          320 t
ORIGIN      
        1 ggtgctggtg gcaattctga ttatgctgcc gactctcggc atcggtgata tgaacaatct
       61 ctataaatgg ctgttgaatc ttaactcggt agtgatgccg ctgcgttatt tatgggtatt
      121 tgttgcattt attgcagtcg ttcgcctggc gcagaaatat aaaccagaat atgtctttat
      181 tcgtaataag cctctggcaa tgaccgtcgg gatttggtgt tttgccttta ccgcctgtgc
      241 ctgtttgacg gggatcttcc cgaaaatgga agccttcact gcagagtgga ccttccagtt
      301 ggcgctgaat gttgcaacgc cgtttgtgct ggtaggatta ggactgatat tcccgctgct
      361 ggcacgtaaa gcgaatagca aataattatt gtggtcggtg acgctgactc tggcagatct
      421 gacccgtttt tacataatcg agaaatgcgc gcaagccgca ggacatatac tggcggtttg
      481 ggtaatagac ttggaagcct ggacgctctg tgatccagtc ttccagtaca cattctaaac
      541 gaccagtatc aaggtactcc ttgatctgtt catagagcag atacccgata ccagcaccca
      601 tcaaaacggc ctccagttca gcatcaacat catccagaat gatatttcct gcgacagcaa
      661 tttccagctc ctttgcaaat tgccagtgga aaggtttgcc gctgggataa cgaaaaacaa
      721 cacattgatg attaagcaga tcatgaggat ggcggggttt gccatatctt gcaaagtact
      781 ctggcgttgc tgctacacat aatttcactg gtgggccgat cgccacgctg atcatatctt
      841 tttcgacgat gccactaagt cgaacgccag cgtcaaagcc ctgttggacg atatcgataa
      901 gagaatcgtc agttgtgagc tctactttaa tatcaggata ttcacgtgtg aaaccaacca
      961 gtaaaggcat taaaaatata cgtgcagcta cccgggcggc attgattttg agcgttcctg
     1021 tcggtgtcag acgaaaatca ttcatctcat ctaacatgat ctgaatttcg tcgaaagctg
     1081 ggcgcaatcg ttcatagaga ttagaacccg cttccgttaa cgatacgctt cgggttgtcc
     1141 gattgaataa gcgaa
//
LOCUS       DADMGC010000322         1155 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.329, whole genome shotgun sequence.
ACCESSION   DADMGC010000322 DADMGC010000000
VERSION     DADMGC010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.329"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(569..748)
                     /locus_tag="JNZ73_004348"
                     /pseudo
     CDS             complement(569..748)
                     /locus_tag="JNZ73_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            842..1155
                     /locus_tag="JNZ73_004349"
                     /pseudo
     CDS             842..1155
                     /locus_tag="JNZ73_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          318 a          212 c          298 g          327 t
ORIGIN      
        1 tagggtagtc actatatttg cgactgagct ggtcgctatt tttaaccagg ctgaaaaatt
       61 gagtcaatat atcgaaagcg gtagttggcg ggcataagta tgtaatgatg attctgcact
      121 ggtgttgaat attattcacg ccaaactttc ctcattgagc aatgtcgtcg caagtggcct
      181 caatgatttt agaccattat cagaggtccg gagcggcggg aagagattta ttgctgtgga
      241 ataattgtgg tactgatgat tatttatatc ccggattggt agggaaaagc ttttttcatg
      301 cactattttt gcaaatattt ctatatggga agcggttttg ttacagatta aatttataaa
      361 gaaatgcatt tggggtgttg ggttgaagag tagctggtag atgttattgt gccagcaggg
      421 gcaggggaat gtgccgtatc gatatgcttt ggcaattgcg tgtgccgcag atggagatga
      481 aatgccaata aaaaagcctg agttcgatga gggacgaact caggctggtg ttatgtttta
      541 cagtaacacg cctcggcgcg ttggctgatt agaactggta gacgatacca actgccagtt
      601 ggtcgtcggt gttcagtcca tgagcgagaa cataatcact cttatcaagc aggttgatct
      661 gataagcggt atatatgttc atattcttat tgaagtaata ccaggtaccg atttcaatat
      721 agttcagacg atcagaatct ttgtaacctg tagactggcc ccctgaatct ccagacaacc
      781 aatatcactt atttaagtga tagtcttaat actagttttt agactagtca ttggagagca
      841 gatgattgat gtcttaggac cggagaaacg cagacggcgt actacacagg aaaagatcgc
      901 tattgttcag cagagttttg aaccgggaat gacggtctcc cttgttgccc ggcaacacgg
      961 tgtggcagcc agccagttat ttctctggcg caagcaatac taggagggaa gtcttactgc
     1021 tgtggccgca ggagaacagg tcgttcctgc ctctgaactt gctgctgcca tgaagcagat
     1081 taaagaactc cagcgcctgc tcggcaaaaa aacgatggaa aatgaactcc ttaaagaagc
     1141 cgttgaatat gggcg
//
LOCUS       DADMGC010000323         1137 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.330, whole genome shotgun sequence.
ACCESSION   DADMGC010000323 DADMGC010000000
VERSION     DADMGC010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.330"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..91)
                     /locus_tag="JNZ73_004350"
     CDS             complement(<1..91)
                     /locus_tag="JNZ73_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY8734351.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVL"
     gene            complement(91..690)
                     /locus_tag="JNZ73_004351"
     CDS             complement(91..690)
                     /locus_tag="JNZ73_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY8734352.1"
                     /translation="MAHIQLVKQTSSGLLLPATSESCEFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYRTPVGGAITPRERELVSGFVEYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
     gene            complement(757..1017)
                     /locus_tag="JNZ73_004352"
     CDS             complement(757..1017)
                     /locus_tag="JNZ73_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734353.1"
                     /translation="MKIEELREIFSEDGLYTVRVENGAIVSHCRIKCLQSQQRKSGAA
                     LIHLVDGLVTDGFLLCGDEFVTSLRALKETGLKAGFSAFECE"
BASE COUNT          287 a          311 c          248 g          291 t
ORIGIN      
        1 ccagcacaga agtttcggga ttgtgattgc agtagccagg aattctgacg gtgcacatct
       61 gcccccgcgc cgctttacgt aaatccacca ttacgcaaac tccagtaact gtgcggccac
      121 attttcgact tcctccggag aggaaaattt acggaacagg atccagttcc acagcacatt
      181 cagtacagat ttataaacct gctgaaactc ggtttcgtcc atgttcgcaa atgcgataga
      241 ttttgccctg cgcccacggc taccgtccgg ataaatatgc tcggtgtaaa atccggcctg
      301 aatggttacc cactcgcgga aagcctcaaa cgactttagc aatgccgtat cccgggttct
      361 gcgtgtcgca acggtattca gatattgctc tgcggcttcg ctcagagctg gcgtatgttc
      421 ccgacctact gattcgcaca ggtactcaac gaagcctgat accagttctc gttctcgagg
      481 cgtgatcgcc ccaccgaccg gagtccggta atcgaatccc agttgcagga gtttgaaaaa
      541 acgcttgtgg aatgcgtagt tacgaacgcg cttaaaatca gcgtgtatcc actcgcctgt
      601 tttgatttga tgcagaaatt cgcaactctc cgacgtcgcc gggagaagta atccggaaga
      661 ggtttgtttg accagttgta tatgtgccat cggctttctc cggtggcacg gtgttactca
      721 gcagggggtc aaccctgcac tgaattgtag atgaactcac tcacattcaa aagcagaaaa
      781 gccagcctta agtccagtct ctttcagcgc ccgtaatgac gtgacaaatt catctccaca
      841 caaaagaaaa ccatccgtca caagcccatc caccaaatga attaacgcag ctccactctt
      901 cctttgttga gactgtaaac atttaatacg gcagtggctg acaatagcgc cattctcaac
      961 gcgcacagta tagaggccat cttcactaaa aatttcacgt aattcttcga ttttcatcaa
     1021 cagaatcctt ccaggtaaat agcactcccc tgttcggggg ccatccctct tatccctgcg
     1081 cgctacttaa gtgtttttga ttctattcct gcgccttctg gaagttcaaa cgagttg
//
LOCUS       DADMGC010000324         1121 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.331, whole genome shotgun sequence.
ACCESSION   DADMGC010000324 DADMGC010000000
VERSION     DADMGC010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.331"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..>1121
                     /gene="pdeF"
                     /locus_tag="JNZ73_004353"
     CDS             <1..>1121
                     /gene="pdeF"
                     /locus_tag="JNZ73_004353"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY8734354.1"
                     /translation="TYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVFLFYLP
                     LALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGGYRVFT
                     PRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMPFNLGT
                     LINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFALWLLAL
                     GALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMISIHSY
                     QNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDPVVHLP
                     NVRALNRALRDALWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT          245 a          254 c          251 g          371 t
ORIGIN      
        1 caacttatat aaaaatacgt gataaatggt gggggcttcc gctgttcctg ccttctttaa
       61 tcttgcccat tttcgcccac attaatactt tcgcgcatat ttcttccggt gaggtttttc
      121 tcttttatct gcctttggca ctgatgatca gcatgatgat gtttttcagc tgggcggcat
      181 tgccagggat caccttaggg atttttgtcc gcaaatatgc agagctgggt ttttacgaaa
      241 cgctctcatt aacggctaat tttattatca ttatcattct ctgttggggc ggttacaggg
      301 tctttactcc ccggcgtaac aacgtttcac atggtgatac ccgtttaatt tcccagcgta
      361 tattctggca gatagtgttt cctgcaacgc tgtttctgat acttttccag tttgctgcat
      421 ttgtaggatt actggcgagc agagaaaatc tggtcggtgt catgcctttt aacctcggga
      481 ctttaatcaa ttatcaggcc ttactggtgg gtaatctgat cggtgtcccg ctgtgctact
      541 tcatcattcg ggtagtgcga aatccatttt atttacgtag ctattattcg caattaaaac
      601 agcaggttga tgccaaagtc accaaaaaag agttcgcgct ctggctactg gcattaggtg
      661 ctttattgtt gctgttatgc atgccgttaa atgaaaaaag cacaattttt agcaccaatt
      721 ataccttgtc attattgctg cccctgatga tgtggggagc aatgcgctat ggttataagc
      781 tgatttcgct gctctgggcg gtcgtgttga tgatcagcat ccacagctat caaaattaca
      841 ttcccattta tcctggctat accacgcagc tgaccataac ctcctccagt tatctggtat
      901 tctcttttat tgtcaattat atggctgtac tggcaactcg tcagcgagcg gtagtcagac
      961 gcattcagcg gcttgcgtat gtggacccgg tggttcatct gccaaatgtt cgcgccctga
     1021 atcgcgcgtt gcgtgatgcc ctctggtctg cgctttgtta tttacgcatc cctggcatgg
     1081 aaatgctggt taagaactat ggcatcatgc tgcggattca a
//
LOCUS       DADMGC010000325         1116 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.332, whole genome shotgun sequence.
ACCESSION   DADMGC010000325 DADMGC010000000
VERSION     DADMGC010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.332"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..565
                     /locus_tag="JNZ73_004354"
     CDS             <1..565
                     /locus_tag="JNZ73_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HAY8734355.1"
                     /translation="IVGRTYNQDTMPPWGLPGMASQSGIFSHSLQGGPTNGNMLRFDD
                     KTGAEEVKFHAEKDLNTTVKNNETHTVMVDRIKTIVKNETISVGEDRNATITKNDGLS
                     VKLAQTINVGTTYRLDVGDQFTLRCGNAALVLHKDGSIEFCGKQLLLHTSDVMQLIGS
                     GIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            588..1032
                     /locus_tag="JNZ73_004355"
                     /pseudo
     CDS             588..1032
                     /locus_tag="JNZ73_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT          309 a          281 c          278 g          248 t
ORIGIN      
        1 catcgtgggg cgcacctata accaggacac catgccgccg tgggggctgc cgggcatggc
       61 gtcgcagagt gggatattca gccactcatt gcagggaggg ccgactaacg gaaatatgct
      121 gcgcttcgat gataaaacgg gtgcggaaga ggtgaagttc catgcggaaa aagacctcaa
      181 taccacggta aaaaacaatg aaacccacac ggtcatggtc gatcgcataa aaaccattgt
      241 caaaaatgaa accatcagcg tgggagaaga ccgtaatgcc accatcacaa aaaatgatgg
      301 tctttccgtc aaactggctc aaaccatcaa tgtcggaacg acatatcgtc tggatgttgg
      361 cgatcaattt acccttcgtt gcggcaatgc agcgcttgta ttacacaagg atggctcgat
      421 tgagttttgc ggaaaacaac tgttactgca caccagcgat gttatgcaac tcattggcag
      481 tggcattgat atgaatccgg atggcggcac agccatgact gcggatgata ttgccccgtt
      541 acctaccccc gacgtttctg aataaaaaat ataaaaggaa tttttctatg accagccttt
      601 accgtattca ggagggctgc tttgccctgc ctgaaacttt tcttgatcgc accgtcaata
      661 tcttcgtccc atcgggcaat gaaagggcaa ctcccagtct gaatatttcc cgcgatacgc
      721 tcaggccaga tgaaagtctg accacctata ttgaccgcca gatagccttg atgaaaaaaa
      781 aacctgggcc agcaccgggt actttcgcgg ggaacggcac tggcgggaac tggcgaagct
      841 gccatgacag gtgaacagat tctcgcaact catcgttctg gaaaaacaga agtgtaccag
      901 cgtcaggccg ggtttatcac cgggccagca aaggtactaa tgttgacgct gaccactcag
      961 cgtccttttg atgatcacac cgaccagcta tggactgcat ggttgactag ttttcagcct
     1021 gctaaaagct aaaaaaaaac agggaggttt tgtcatgtat gaagccgcac gggtggatga
     1081 ccccatctac cacaccagcg cccttgccgg gttttt
//
LOCUS       DADMGC010000326         1107 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.333, whole genome shotgun sequence.
ACCESSION   DADMGC010000326 DADMGC010000000
VERSION     DADMGC010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.333"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..387)
                     /locus_tag="JNZ73_004356"
     CDS             complement(<1..387)
                     /locus_tag="JNZ73_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY8734356.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQVDLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNV"
     gene            816..1097
                     /locus_tag="JNZ73_004357"
     CDS             816..1097
                     /locus_tag="JNZ73_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734357.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          308 a          249 c          251 g          299 t
ORIGIN      
        1 aacgttaggt tcacgcttat aatcggccgg tgcaatcgta tagccgttta caggcgctat
       61 cagcgatgag cataaaaaat ggttatcacg ggcatagatc aattcattaa tataaagata
      121 gccacgaata atattcaaca ttcgcttttg atgggctgga gtgcaaactt gcccctgata
      181 gcgttcagct gcatcgctca ctaaatctac ctggcgaatg accagctcgg atttatccag
      241 tgccagttga gcaaaggttc ggagctgatt attcacttct gattttgccc aaaggaaggc
      301 aatccatagc gaaagaatga tgggaaagag aactaaaaag ataatgcccg gcaacgccag
      361 taattggtgt cgtgcacgat gactcattgc tatcaatttc ctgcctgttt atacccggcc
      421 attgcaaggt gaaaccctgg ctaccgacgt ctttagataa atcttacggt ataaatagca
      481 cttttgcctg atttgaaatt gatacctttt taatcgtcac ctggatgaaa ataattcatt
      541 gatattcaaa attaattttc tacaggaata cagaggcggc gggaagatat gcccttaatg
      601 ccactgaaaa accacataat ctgtacagca ataaaagtca cggcctaata tggctaacaa
      661 acaatcaatt gtgatatggt tcacaaaatt aatgaaacaa acagagtgtt tcatttttgt
      721 gttctgcaag ccccgcgata gccagagtgt accacgcctc ccgtgaacaa cgccgcgctg
      781 tccagggcat cggctctttt cagaggaatt gtttgatggc taccctcacc actggcgtgg
      841 ttcttcttcg ctggcaactt cttagtgccg taatgatgtt tctggccagc acactcaaca
      901 tccgttttcg tcggtctgat tatgtcgggc ttgcggtgat cagcagcggt ctgggcgttg
      961 tttccgcctg ttggttcgca atggggttgc ttggcatcac aatggcggac atcaccgcta
     1021 tctggcacaa catcgagtcg gtgatgatag aagagatgaa tcagacaccg ccacaatggc
     1081 caatgatttt gacctgatac gtagaag
//
LOCUS       DADMGC010000327         1093 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.334, whole genome shotgun sequence.
ACCESSION   DADMGC010000327 DADMGC010000000
VERSION     DADMGC010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.334"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            101..1093
                     /locus_tag="JNZ73_004358"
                     /pseudo
     CDS             101..1093
                     /locus_tag="JNZ73_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          296 a          279 c          294 g          224 t
ORIGIN      
        1 atagctgcgc ggaatagtag atccctttga gggaacttag cccggattgt gcgatctgat
       61 caatcgccaa atcaaaacaa atcaccaacc ggactgagca atgccgatca tagcaccaat
      121 ttcccgtgac gaacgacgcc tgatgcagaa agccatccat aaaacacacg ataaaaatta
      181 tgcccgcaga ctgactgcca tgctgatgct gcaccggggc gaccgtgtca gcgacgttgc
      241 cagaacgctc tgctgcgccc gttcctctgt tggacgctgg attaactggt tcacgcagtc
      301 gggtgttgag ggactgaaat cattacctgc cgggcgtgcc cgtcgctggc cgtttgagca
      361 tatctgcaca ctgttacgtg agctggtaaa acattctccc ggcgactttg gctaccagcg
      421 ttcacgctgg agtacagaac tgctggcaat aaaaatcaat gagataaccg gttgccagtt
      481 aaatgccgga accgttcgcc gctggttgcc gtctgcgggg attgtgtggc gaagggctgc
      541 gccaactctg cgtatccgtg acccgcataa agatgaaaag atggcagcaa tccataaagc
      601 actggacgaa tgcagcgcag agcatccggt cttttatgaa gatgaagtgg atatccatct
      661 tcatcccaaa atcggtgcgg actggcaact gcgcggacag caaaaacggg tggtcacgcc
      721 gggacagaat gaaaaatatt atctggccgg agcgctgcac agcgggacag gtaaagtcag
      781 ctatgtgggc ggcaacagca aaagttcggc gctgttacat cagcctgctg aagcggctta
      841 aagcgacata ccgtcgggcg aaaaccatca cgctgatcgt ggacaactac attatccaca
      901 aaagccggga aacacagagc tggctgaagg agaacccgaa gttcaggggc atttatcagc
      961 cggtttactc gccatgggtg aatcatgttg aacggctatg gcaggcactt cacgacacaa
     1021 taacgcgtaa tcatcagtgc cgctcaatgt ggcaactgtt gaaaaaagtt cgccatttta
     1081 tggaaaccgt cag
//
LOCUS       DADMGC010000328         1085 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.335, whole genome shotgun sequence.
ACCESSION   DADMGC010000328 DADMGC010000000
VERSION     DADMGC010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.335"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..476
                     /gene="ybcL"
                     /locus_tag="JNZ73_004359"
     CDS             <1..476
                     /gene="ybcL"
                     /locus_tag="JNZ73_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HAY8734358.1"
                     /translation="NEIKTGEQLTTSHVFSGFGCEGGNTSPSLTWSGAPEGTKSFAVT
                     VYDPDAPTGSGWWHWTVANIPATVTYLPTDAGRRDGTKLPTGAVQGRNDFGYAGFGGA
                     CPPKGDKPHHYQFKVWALKTDKIPVDSNSSGALVGYMLNANKIATAEITPVYEIK"
     gene            486..>1085
                     /locus_tag="JNZ73_004360"
     CDS             486..>1085
                     /locus_tag="JNZ73_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8734359.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIVNYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPRTKWTANKVARYLYISVSTLHRRLA"
BASE COUNT          341 a          203 c          222 g          319 t
ORIGIN      
        1 gtaatgaaat aaaaacagga gagcaactta caacgtctca tgtattttct ggatttgggt
       61 gtgaaggtgg taatacatcg ccctcattaa cctggtctgg tgctcctgaa ggcaccaaaa
      121 gctttgccgt aactgtgtat gatccagatg ctcctacagg cagtggatgg tggcactgga
      181 ctgttgctaa tattccagca actgtaacat atttgcccac tgatgcagga agacgtgatg
      241 gtacaaaact tccgactggt gctgttcaag gccgaaatga ttttggttat gcaggatttg
      301 gtggagcatg tcctcccaaa ggagataaac cccatcatta ccagtttaaa gtatgggctc
      361 taaaaactga taagattcct gtggattcta attccagcgg agcgttagtt ggttatatgc
      421 ttaatgccaa taaaattgca accgctgaga taacaccagt ttatgagata aagtagggta
      481 agagtatgct ggcaaaagat aagtctaacc taaagattga agaaatacgg atgcataaac
      541 atcatgagat tcacagggtt aagcctctta tgccagcttt atgtcgtatc cgtcagggaa
      601 aaaaagttat caattgggag acgcatagtc taactgttga taataatcag ataatattat
      661 ttccctgtgg atatgaattt tatattgtga attatccaga agcaggtctt tatcttgcag
      721 aaatgcttta ttatcccatt gatctaattg aaaagtttca aaaattttat gcgataactg
      781 atcaaattcg taacacgaca ggtttctgct tacctcagaa ccccgagtta atatattgtt
      841 gggagcaact aaaaacatct atttcccgag gcttctcaac gcaaattcag gaacacttag
      901 caatgggcgt tctactttca ttaggagcac atcatgttaa ttgtttactt ttatcagata
      961 gtaaacaatc attaataagt cgttgttata acctaatgct atccgaacct agaacaaaat
     1021 ggacagcaaa caaggtagcg agatatctct acatttctgt ttccacattg catcgccgtc
     1081 tggca
//
LOCUS       DADMGC010000329         1058 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.336, whole genome shotgun sequence.
ACCESSION   DADMGC010000329 DADMGC010000000
VERSION     DADMGC010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.336"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            70..588
                     /locus_tag="JNZ73_004361"
                     /pseudo
     CDS             70..588
                     /locus_tag="JNZ73_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          324 a          238 c          221 g          275 t
ORIGIN      
        1 tgctgaatca gaactttacg cccacagcac ctaatcaggt ctgggtcgca gattcagttg
       61 tccaggcctt ccgaaatcaa ccgactgaag gcgctggtcg cgaaactgca gcgtatgcag
      121 ttcggtaaaa gctcagaaaa acttcgcgca aaaaccgaac ggcggataca ggaagcacag
      181 gagcgaatca gcgcacttca ggaggaaatg gcggaaacac tgggtgagca atatgacccg
      241 gtactgccat cttccctgcg ccagtcttca gcccgtaaac cgttaccagc ctcacttccc
      301 cgtgcccccc gggtcatccg gccggaagag gaatgctgtc cggcctgtgg tggtgaactc
      361 agtccactgg gatgtgatgt gtcagagcag ctggagctta tcagcagcgc ctttaaggtt
      421 atcgaaaaac aacgccctaa actggcctgt cgccggtgcg accatatcgt gcaggcaccg
      481 gtgccttcaa aacccattgc acgcagttat gccggagcgg ggcttctggc ccatgttgtt
      541 accggaaagt atgcagacca tctgccatta taccgccaaa gtgatctact attccacaca
      601 gctatttagc tctctttttg ttgagctccc atgattcttt catttattgt tactaattat
      661 tttatgttat catgatgtta gaacacgctc tgctctgtgt ctctatttta tctatcatat
      721 tctgaggatt aactacataa caaggcgcaa gatatatatc caaaaacaaa taataccgtt
      781 gcaataattc ctttcagtaa aggaggtgtg attaatttca ggggatttca gagtaaaaat
      841 tgtacaacat attaaaatat gttcatttcg ttataataca cacaagaaaa tattaagtta
      901 gataatatat tctaaataac catagataaa aacgcacata atggggaata ttttatgaaa
      961 atacctgaag cagtaaatca tattaatgtt cagaataata ttgatcttgt tgatggaaaa
     1021 acgaatccga ataaagcaac aaaagcatta caaaaaat
//
LOCUS       DADMGC010000330         1043 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.337, whole genome shotgun sequence.
ACCESSION   DADMGC010000330 DADMGC010000000
VERSION     DADMGC010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.337"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..1013
                     /locus_tag="JNZ73_004362"
     CDS             <1..1013
                     /locus_tag="JNZ73_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8734360.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGSKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
BASE COUNT          243 a          229 c          271 g          300 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa tgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggcagtaaaa accgctttac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc ttaccggaga ggg
//
LOCUS       DADMGC010000331         1025 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.338, whole genome shotgun sequence.
ACCESSION   DADMGC010000331 DADMGC010000000
VERSION     DADMGC010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.338"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..>1025)
                     /locus_tag="JNZ73_004363"
     CDS             complement(<1..>1025)
                     /locus_tag="JNZ73_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8734361.1"
                     /translation="EKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSWVFIFAL
                     YFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLAQKPQAI
                     LIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVAGIAMFV
                     MSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGAEKISPY
                     VNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQYYAFQKL
                     GEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCRTNASGT
                     PVNGYFL"
BASE COUNT          303 a          269 c          219 g          234 t
ORIGIN      
        1 tcagaaaata gccattaacg ggcgtaccag aagcgttggt acgacataaa ctggctggaa
       61 tatatttgct gtcagcatca cctaatagca ctttaagcgg ggcatcaatc gagaatacca
      121 gcgcagctac ttgtcccagg gtattcgcaa tggcgtaaat caccattaaa gtattaccca
      181 tgttgtaata ctcgcccagc ttctgaaagg cgtaatattg accgttcgtc attaagtcat
      241 ccgggatatt acgcgagtca aacatcatcc ccatcgccag tgagcccaga atggcacaaa
      301 ccgcaaccat caccgccagg catagcatcc cttttggaaa ttcttttcct gggttgcgcg
      361 tttgattaac gtaaggagaa atcttctctg ctccgccaac cgcgaaaacc agcattgaaa
      421 tagtggtaat gtaggtaaag tcgatatgag gaatgaacgt ttcccaggta atgtttgtgg
      481 tcgcaatatg cacttcagta atcgcaggcg cggttaccgc catcgccaca tacaggagcg
      541 acataacaaa catcgcaatc cctgccacag aaccgacgat tttcagcgat ttcataccgc
      601 gtgaagcaac ccacataaag aagataaaca gcaccagcgt taacccctgt aacgctacga
      661 ctgaatattc tttgattagc gaaccgtcgc ctttcatcgc ccaaccgagc gcaatcagaa
      721 ttgcctgggg tttttgtgcc agatagggaa tatgcaccac ccagtaggtc cacgcggcga
      781 gataagccag tccgggtccc atcgtgtgtt taatccaggt actgaccccg cccttcccat
      841 ctttgaaggt cgagcctaac tgaccaacaa ttagcgcata aggtgtgaaa tagagtgcaa
      901 agataaacac ccatgaaaaa acaaccacca gcccctggtt ggcatagtta ttaacaacgt
      961 tgccaaaacc ccagacagtg ataaaagaca tcagcgcaat gttgtaccat cgcaactgtt
     1021 tttcc
//
LOCUS       DADMGC010000332         1018 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.339, whole genome shotgun sequence.
ACCESSION   DADMGC010000332 DADMGC010000000
VERSION     DADMGC010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.339"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..767
                     /locus_tag="JNZ73_004364"
     CDS             <1..767
                     /locus_tag="JNZ73_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY8734362.1"
                     /translation="FATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRHQ
                     QTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG"
     gene            788..>1018
                     /locus_tag="JNZ73_004365"
     CDS             788..>1018
                     /locus_tag="JNZ73_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY8734363.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQET"
BASE COUNT          332 a          225 c          231 g          230 t
ORIGIN      
        1 aatttgcaac agataatggt tatacatcag gaaatccatt taacggtctg acaccattaa
       61 aaaagtccaa gatagaacca gatcccctca cccgtgacga attcattcgt tttattgagg
      121 cttgccgtca tcaacaaaca aaaaacctgt ggattatcgc tgtatacacg ggtattcgtc
      181 acggggagct ggtatcactg gcctgggaag atatagacct taaagcgagg actataacca
      241 tccgccggaa ttatacaaaa ctaggcgaat tcactccacc aaaaactgat gctggtaccg
      301 gaagaacaat tcatctagtt caaccagcta ttgatgctct taaaagccaa gcggaaatga
      361 ccatgcttgg aaaacagcat tctgtagagg taaagcagag ggaatatggg agaagtactg
      421 tgcataaatg cacttttgtt tttagtcctc aggtaataaa acagcggcag ttttccggac
      481 cgcactataa ggttgactcc atcagggagt catggacaag tatcttaaaa cgcgcaggtc
      541 tgagacacag aaaatcgtat caatccaggc atacctatgc atgctggtca cttgccgcag
      601 gagctaatcc tagttttatc gcaagccaga tgggccacac aaacgcacaa atggtattca
      661 atgtttacgg agcatggatg aaagacaaca atcacgaaca gatagaactt cttaacagaa
      721 gactatctga aagtgtccca tgcatgcccc ataagaaagc ggggtaaaat aaaaacttgc
      781 aaaatcaatt ggtttacctt taatccctgt cacgttacgc gcgtggcaga ggcgttacgg
      841 attgctgaaa ccgcaacgga cagacggcgg tcatcggctg ttcaacgatg ccgatattga
      901 ccggatccgc gagatcaaac gctggatcga caacggcgtg caggtcagca aagttaaaat
      961 actgctcagt aatgaaaatg ttgatgtgca gaacgtctgg cgcgatcagc aagaaaca
//
LOCUS       DADMGC010000333         1001 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.340, whole genome shotgun sequence.
ACCESSION   DADMGC010000333 DADMGC010000000
VERSION     DADMGC010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.340"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            1..1001
                     /locus_tag="JNZ73_004366"
                     /pseudo
     CDS             1..1001
                     /locus_tag="JNZ73_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          266 a          238 c          272 g          225 t
ORIGIN      
        1 attcttccac taataaagaa cagtcagatg ctcaggccga gatccgccgt ctccagaaag
       61 agctgaaacg ggttaccgac gaacgggaca tattaaaaaa agccgcggcg tacttcgcaa
      121 agctgtccga ctgaggtacg cctttatccg tgacaactcc tgttgctggc ctgttcgcct
      181 gctctgtcgg gtgctggatg ttcatcccag tggtttttac gcctggcttc agcagccgca
      241 ttcacaacgc catcaggcag acctgagact gacaggacag attaaacagt tctggctgga
      301 atcgggatgc gtctatggtt atcgcaaaat ccatctggat ctgcgtgaca gcgggcaaca
      361 gtgcggagta aacagagtct ggagactgat gaaacgtgtc ggaataaagg ctcaggtcgg
      421 ataccgaagc ccgcgggcac gtaaaggcga ggccagtatc gtgtcaccca acaggctcca
      481 gcgacagttc aatccggatg ctcctgatga gcgttgggta acggacataa cctacatcag
      541 gacccacgaa ggctggctgt atcttgccgt tgttgttgat ctgttctcac gcaaaattat
      601 cggctggtcc atgcaatccc ggatgacaaa ggacattgtc ctgaacgcac tgctgatggc
      661 tgtatggcgg cgtaatcccg aaaaacaggt gctggttcat tcggatcagg gcagtcagta
      721 cacaagccat gagtggcagt cgttcctgaa atcacacggc ctggagggta gcatgagccg
      781 tcgcggtaac tgccatgata atgcggttgc agaaagtttt ttccagttgt tgaaacgtga
      841 acggataaag aaaaagatct acggaacgcg ggaagaagcc cgcagtgata tttttgatta
      901 catcgaaatg ttttataaca gtaagcgtcg gcatggttct agcgaacaga tgtcaccgac
      961 agaatatgaa aaccagtatt atcaacggct cggaagtgtc t
//
LOCUS       DADMGC010000334          989 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.342, whole genome shotgun sequence.
ACCESSION   DADMGC010000334 DADMGC010000000
VERSION     DADMGC010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.342"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            1..989
                     /locus_tag="JNZ73_004367"
                     /pseudo
     CDS             1..989
                     /locus_tag="JNZ73_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          232 a          240 c          302 g          215 t
ORIGIN      
        1 ccccgaaatc cgtcaacggg cagttcgtat ggttctggaa agtcagggcg aatatgactc
       61 acaatgggca gcaatttgct ccattgctcc aaagactggc tgtacgccgg agactctgcg
      121 tgtctgggtt cgccagtatg agcgggatac cgggggcgga gatggagggc tcaccagtgc
      181 tgaacgtcag cgtctgaaag agctggaacg tgaaaatcgt gaactgcgcc gcagcaacaa
      241 tatccttcgc caggcttccg cttattttgt gaaggaggag ttcgaccgcc tctggaaaaa
      301 atgatgccac tgctggataa gctgcgtgag cagtacggag tcagaccggt atgcagcgaa
      361 ctgcatattg ccccgtcaac gtattaccat tgtcagcaac agcgacatca tccggataaa
      421 cgcagtgccc gtgcgcagca cgatgactgg ctgaagaaag agatacagcg cgtatacgat
      481 gaaaatcatc aggtgtacgg tgtgcgtaaa gtctggcgtc agttgttacg ggaaggaatc
      541 agggtggcca gatgtacagt ggcgcgcctc atggcggtta tgggacttgc cggtgttctc
      601 cggggtaaaa aggtccgcac taccgtcagc cggaaaaccg ttgccacagg tgaccgcgta
      661 aaccgtcagt tcgtggcaga acgtcctgac cagctgtggg tggctgattt tacttacgtc
      721 agcacatggc agggcttcgt ctatgtggcg tttatcattg atgtgtttgc cggatacatc
      781 gtggggtggc gggtctcatc gtctatggaa acgacattcg tgctggatgc gctggagcag
      841 gcgttgtggg cccgtcgtcc gtctggcacc atccatcaca gcgataaagg ctctcagtat
      901 gtgtcactgg cctatacgga gcgactaaaa gaagccggat tactggcatc aacagggagt
      961 acaggcgact cgtatgacaa cgcgatggc
//
LOCUS       DADMGC010000335          983 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.343, whole genome shotgun sequence.
ACCESSION   DADMGC010000335 DADMGC010000000
VERSION     DADMGC010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.343"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JNZ73_004368"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JNZ73_004368"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HAY8734364.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLHTDYVDLTLIHWPSPN
                     DEVSVEEFMKALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          259 a          219 c          221 g          284 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctt cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtatgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DADMGC010000336          980 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.344, whole genome shotgun sequence.
ACCESSION   DADMGC010000336 DADMGC010000000
VERSION     DADMGC010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.344"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
BASE COUNT          284 a          179 c          252 g          265 t
ORIGIN      
        1 tgacacttgt tgactacctg tgcggcatct ggtatcaagg tggcgtgaag ccggttcagg
       61 ttctcatgcg gaacgttgga acctatatcc ccccgatacc aaaggcgaaa tgtaagtgac
      121 aaaatgcaat gaacacgttc tccgttaact gaggagcatg gtgataaata aaggtaaatg
      181 cagaaggtgt agagtctcgc agatttctga catcatagag cctaacatta gatgcataac
      241 gctacagatt gtagaatctc tacaggtgaa atatacactg gttggcgttt atgtcgcaaa
      301 acctgtcatt caggttcatc tcaccaataa ggatatgagt gaagtggagg ataagtgagt
      361 ctgctgtcag agttttctgg tgtatgtcag taactgcgaa ccatgtctgc ttgatatggg
      421 agcttatgga ggctctctgt acaaggttag ggagctaaca aagcagggat aaaaagtccg
      481 cctgatgcag gcacggttcg ttaaatcttt tgccattggt aacaaaatgg tgcgatgaat
      541 gttcaggcta tctttatctt gatggtggtt cagctgtttg gtaaagaaat cgctgtaaca
      601 atagaagaac tgcaggcagt actggtccca catcgtttgc tcagtcaact ggtgaagttc
      661 cgtagcgcgt aaattaatgc tttgcatgaa acgttactgg agtggatgaa agaatacctg
      721 catatatcat tactgcgttg gattaccagt acaacagatt gaatgaactg gacatgttga
      781 tagacggcat tgaaaagcag cttataagca tagtgcggca gagttaattg gagagcactt
      841 cgccttctga gtattaagta agcatggtgg tacctgcgga tactatctat tcactgtgct
      901 cggtcggcag ccttactgac caaggagttg ggggagttga taacaaagtt gaaaaaacag
      961 ctctcaacca aggtggttat
//
LOCUS       DADMGC010000337          979 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.345, whole genome shotgun sequence.
ACCESSION   DADMGC010000337 DADMGC010000000
VERSION     DADMGC010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.345"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            89..>979
                     /locus_tag="JNZ73_004369"
     CDS             89..>979
                     /locus_tag="JNZ73_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY8734365.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGGIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKAQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     EQIKPFLEKAMTMYNDVLFPG"
BASE COUNT          259 a          230 c          243 g          247 t
ORIGIN      
        1 tagttgttta ctgtgcgctg ttgtccatgt gatattgtgt accagatgtg tgccaaacta
       61 ttatttctga gcgtaccaaa tattatttat ggctataagc gacacaaaac tgcgtactat
      121 ttatggtaaa ccatattcgg gcccacaaga agtggctgat gccgatggcc tcagcgtacg
      181 aatttcaccg aaggggggca tccagttcca gtaccgctat cgctggcatg gcaagcctaa
      241 tcgacttggg cttggtcgat acccatccct gtctttgaag gatgccagac agatcactgc
      301 tgacttgcga aagctctatt tctcaggaac ggatccacgc acctattttg aagagaaggt
      361 ggagaactcc atgacggtcg cccagtgtct cgactactgg ttcgacaact acgtctctac
      421 aactctcaga gaaaaggccc aggcacttta tcgatcaacg gttatgaagc gcatgcatga
      481 cgccttccct aatcgtccgg catcttctat cacggttaag caatgggttg acctgcttac
      541 cgaagaagaa aaagataatc cacgccgagc aaggcaggtg ctaagtcaac taagatcagc
      601 aattagttgg tgcatgcggc gtcagttgat agatagttgc gcaattatga gcatccagcc
      661 aagggacttc ggctcccgcg ctgaggtagg ggatcgggta ctgtcgtatc acgaactggc
      721 taagatttgg cttgctattg aaagaagccg tgcgtctacg tcaaataagc tccttcatca
      781 gatgcttatg ctgtgggggg cgaggctctc agagcttagg ctggcaacaa agacagaatt
      841 tgacctgctg gacaacgtat ggaccgtacc gaaagagcat agcaagatgg gtaatgttat
      901 ccgccgtcca atcttcgaac aaattaagcc ttttctcgaa aaggccatga caatgtacaa
      961 tgatgttctt ttccctgga
//
LOCUS       DADMGC010000338          964 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.346, whole genome shotgun sequence.
ACCESSION   DADMGC010000338 DADMGC010000000
VERSION     DADMGC010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.346"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            341..685
                     /locus_tag="JNZ73_004370"
     CDS             341..685
                     /locus_tag="JNZ73_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734366.1"
                     /translation="MDTSRDNSELFANDGTPTWLKEKRAKEIALDEIIELLNKHHGGT
                     SNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNALWIELEGVEYAFPDPQTQNE
                     TQQAGYDENIPV"
     gene            736..924
                     /locus_tag="JNZ73_004371"
     CDS             736..924
                     /locus_tag="JNZ73_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HAY8734367.1"
                     /translation="MYKITATIEKEGGSPTSWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLLSS"
BASE COUNT          335 a          197 c          216 g          216 t
ORIGIN      
        1 gtggcgacgc tttaccgatc gatttgttaa cagtcaggcg cacattatta tgtgcgggcg
       61 tgcaggatat gagtatgact ttttcgaagg tgatgatggc aaacgccagt tggcaaaaac
      121 cggtataaaa atgaaagcag aaacagaaac cggttatgaa ccctccatcc tgatccagat
      181 ggagaagcaa atgaacatag aaaccggtca ggtgtggcgc accgccagaa tactaaaaga
      241 tcgctccacc cgtattgatg gacaggtatt ttcaaaccca acatttaaaa actttcttcc
      301 tcatatcgag tctcttaacc tgggaggtga acaccccggc atcgatacct cacgggataa
      361 cagtgaatta tttgccaacg acggtacacc gacatggcta aaagaaaagc gtgcaaaaga
      421 aatcgcactt gatgaaatta tcgagttgct caacaagcac catgggggca ccagtaacga
      481 cgccaaacgc gcaaaagctg atttgctgga aaaaacattc acctcccgct catgggaaag
      541 aatcaaaggt atggactggc cgacaatcaa agctggtcgt aatgcactat ggattgaact
      601 ggaaggcgtt gaatatgcat tcccggatcc acaaacacaa aacgaaacgc aacaagcagg
      661 atacgatgag aatattccgg tgtaaaacca agtgcatttt gaacacggtt tttgaaaata
      721 ttaatggagg tgttcatgta taaaattaca gccactattg aaaaggaagg tggttctcct
      781 accagctgga caagatattc aaaatctaaa ctaacgaaat cagaatgcga aaaaatgctc
      841 tcagggaaaa aagaagcagg cgtttccaga gagcagaaag taaaactgat aaattttaat
      901 tgcgagaaac ttctgtcctc gtgagttgca ttatatcaaa attagaactt catagctgat
      961 tgag
//
LOCUS       DADMGC010000339          959 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.347, whole genome shotgun sequence.
ACCESSION   DADMGC010000339 DADMGC010000000
VERSION     DADMGC010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.347"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            57..554
                     /locus_tag="JNZ73_004372"
                     /pseudo
     CDS             57..554
                     /locus_tag="JNZ73_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            595..786
                     /locus_tag="JNZ73_004373"
                     /pseudo
     CDS             595..786
                     /locus_tag="JNZ73_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          248 a          238 c          269 g          204 t
ORIGIN      
        1 gccagccccg tcccgcccga tagagcgcgg catagcgggc cccggattac ttgcgtgcga
       61 cggcagaaga agccctgaag cgaatcagtg aactgtacgc catcgaggat gaaatacggg
      121 gattaccaga gtcagagtgt cttgcagtca ggcagcagcg aagcaaagcg ttactgacgt
      181 cgctgcatga atggatggtg gagaagaatg gcacgctgtc gaaaaaatcc agactgggcg
      241 aagcattcag ctatgtactg aatcagtggg acgccctctg ttattacagt gatgacggtc
      301 tggcggaggc ggacaataac acagcggaaa gagcgcttcg tgcagtctgt ctcggaaaga
      361 aaaattacgt gttcttcggt agcgatcacg gcggcgagcg tggtgcactg ctgtacgggc
      421 tgatcggcac ctgccgtctg aacggtatcg atccggaagc gtatctgcgc catattctga
      481 gcgtactgcc ggaatggcct tccaaccgag ttgacgacct cctgccatgg aaggtggtgc
      541 taccatcagg ataatcgtca atacggtgtt cggtgaacgt ttacttggag catcatgctt
      601 catgaagaaa aactggcacg tcatcaacgt aaacaggcga tgtatacccg aatggcagcc
      661 ttcccggcgg tgaaaatgtt cgaagagtat gacttcacgt tcgccaccgg agcaccgcag
      721 aagcagctcc agtcgttacg ctcactcagc ttcatagagc gcaatgaaaa catagtctta
      781 ctggggacat ctgacatcac aaatccccgg gtggggattt gtgtataaga gacagcccat
      841 ggaggaagga gatatatcgc agctctgacg actcactcct tatttacccg ggaaatactt
      901 ctccagcaat gcatacagga tggcagcggt ttcagcatcc attatgccgt cataatttt
//
LOCUS       DADMGC010000340          957 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.348, whole genome shotgun sequence.
ACCESSION   DADMGC010000340 DADMGC010000000
VERSION     DADMGC010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.348"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..>957
                     /locus_tag="JNZ73_004374"
     CDS             <1..>957
                     /locus_tag="JNZ73_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013141603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAY8734368.1"
                     /translation="YIARLRNRVENRLWHSLAACIDDSQTQQLLDLLSVPAGSRYSLL
                     DQLRAGPTKVNATSLVQAIGRLQTIRSLGVTLPAITPVSDIRIAAMARYASTAKITAL
                     QRLPEKRKLATLVAFSCCMEATAQDDALELLEALLRDLFNEAVQADKRNRQRTLKDLD
                     RAAEILAKACRMLLDDKLSDTDVRDSIFNIIPEDVLTHAVNNVTSIIRPDNNVYFNEL
                     DSKFKTVRRFLPDLLSRIHFEGNASAKTLIEALCWIEVNLKKKKTDNDAPREIINKPW
                     QQHVIRKDGSIDFHAYTFCALKELQLALKKRDIYVNPSWRYAD"
BASE COUNT          288 a          210 c          200 g          259 t
ORIGIN      
        1 tatatagccc gattacgcaa cagagttgaa aaccgtctct ggcattcact ggcagcttgc
       61 attgatgact ctcagactca acagcttctg gatcttcttt cggttcctgc cggaagccgc
      121 tattcgttac ttgatcaatt acgcgccgga cctactaaag tgaatgccac atcactggta
      181 caggctatag gtcgccttca gacgatacgt agcctaggag tgacattacc tgcaattacg
      241 ccagtttcgg atatccgtat tgcggccatg gcacgttatg cctcaacggc taagataaca
      301 gcgcttcagc gcttaccaga aaaaagaaaa ctggctacgc ttgttgcatt ctcttgctgc
      361 atggaggcga cagctcagga tgatgctctt gagttacttg aagcgctact ccgggatctg
      421 tttaatgaag cggttcaagc agataaacgt aaccgccaac gaacacttaa agatctggat
      481 cgtgcagcag aaatactggc aaaagcatgt cggatgcttc ttgacgataa attatctgat
      541 actgatgttc gtgatagtat cttcaatatc attcccgaag atgtactgac tcatgcagta
      601 aataatgtga catcaattat tcgaccagat aataacgttt acttcaatga acttgattct
      661 aaattcaaaa cggtgcggcg tttcttacct gacttacttt ccagaataca ttttgagggc
      721 aatgcctcag ccaaaacatt aattgaagcg ctttgctgga ttgaagttaa tttaaaaaag
      781 aaaaaaacag ataacgatgc accacgcgaa ataatcaata aaccgtggca acagcatgtg
      841 ataagaaaag atggtagtat tgatttccac gcctatacat tttgtgcact taaggaactt
      901 cagcttgcac tgaaaaaacg ggatatttat gtcaatccca gctggcgtta tgcagat
//
LOCUS       DADMGC010000341          957 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.349, whole genome shotgun sequence.
ACCESSION   DADMGC010000341 DADMGC010000000
VERSION     DADMGC010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.349"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..98
                     /locus_tag="JNZ73_004375"
     CDS             <1..98
                     /locus_tag="JNZ73_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8734369.1"
                     /translation="YARLGFVQLSATPNAFGAVPMFLAIQHILAA"
     gene            complement(154..951)
                     /locus_tag="JNZ73_004376"
     CDS             complement(154..951)
                     /locus_tag="JNZ73_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY8734370.1"
                     /translation="MAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDR
                     EAPLALGSIYYDGEQVRVDYAKAYALFNQAAQQGMNLAWSRLGMMYANGQYVEVDCKK
                     AKEYLDKGVHIYGGPEDFLATCRKDMIDRKTVEDTLPVITVTRSGMRDNFLDKGFSCM
                     DSLFATTNKLGEVANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTS
                     NSSLILYKPEFERKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
BASE COUNT          236 a          250 c          197 g          274 t
ORIGIN      
        1 tttatgctcg tctcggcttt gttcagcttt cagcgacacc aaatgctttt ggtgctgtac
       61 ctatgttttt ggcgattcag catattctcg cggcttagat tattatcgcc acccgccagg
      121 gcaagttttg ccttagccgg gggctgagca gcgttaccac ttttgttcaa ttgcccccgc
      181 tttcagcaac tccacatcct tgccgttaat agttgccgtt gcggcaacta ttgtggtcct
      241 tacagttgca caagattttc gctcaaattc cggtttataa agaataagag acgaattact
      301 ggtaaatgaa ccaaataggt aatcagtaaa gctaatattc agccggttta aaccgaaagg
      361 cgcgaagcca accgtttggt taatctcctt accggatggg cgacgaatgc tgaatgtcac
      421 gcgaagattg gccacttcgc ctaatttatt ggtggtggcg aagaggctat ccatgcagga
      481 aaaaccttta tctaaaaaat tatcccgcat cccagaacgg gtaaccgtga tcacgggtaa
      541 cgtatcctca accgtttttc tgtcaatcat gtcttttcga caagtagcca gaaagtcttc
      601 cgggccacca taaatgtgga cacctttatc aaggtattcc ttcgctttct tacaatctac
      661 ctcaacatac tgaccattgg cgtacatcat gcccaacctg gaccatgcta aattcatacc
      721 ttgctgcgcg gcctgattaa atagtgcata cgctttggca taatccaccc gcacctgctc
      781 accgtcgtag tagatactcc ccagcgctaa cggagcttct ctgtcacctt gtttagcggc
      841 taactcgaac catttaatcg cttcctgaac atcaggtttg gtatcatagc gaggctgata
      901 atattcccag cctaacaggc tctgcgcgtc gatatctccc gattcagcca ttttatg
//
LOCUS       DADMGC010000342          955 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.350, whole genome shotgun sequence.
ACCESSION   DADMGC010000342 DADMGC010000000
VERSION     DADMGC010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.350"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(105..794)
                     /locus_tag="JNZ73_004377"
     CDS             complement(105..794)
                     /locus_tag="JNZ73_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HAY8734371.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTAHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
BASE COUNT          234 a          255 c          187 g          279 t
ORIGIN      
        1 gccatttttc aggcataaaa aaaccgctcg gcggcgggtt ttgcttactt taccatcgcg
       61 tacaaaatcg gcaaagtatc agatttgcat gaaatatatg ccttttaatc tacttttgca
      121 atactttgct ttgaaaatgc cgctttttgt tctgaacgtg ttctcatcac aaacaataaa
      181 gcctcactat ccagtcggtg aaaaatgtgt ttcattacaa cccagtgagc agtaaatgtt
      241 ttggaccagt ttttggttgt tactcccgcc agtaatgcca gctcctgata ttcataacct
      301 tccccaccaa aaagttctgc ttttaccgcc tgcgccgcca gccagattaa ttttttcagg
      361 cgttcctgtg ttttctctgc aattttccta gtgccagaat gagccttaaa ctcttcccac
      421 gcccagtggg ttatggtgat ctgatactcc cagcaaatac ttccgctgta acaccacaac
      481 aaccaggctt tatggtgctc ttcaagagac agaacagccc gccgccacga tgatgtcgaa
      541 aacgcaacct gactgaccag aggaattgac gcccccttcg ccagtgattg ttttcccggg
      601 attggtggat tatcccgcgt tatcattttt ccggtcacct catcgcggta ccggattttt
      661 ttacgcctgt aacgccctgt atcgaacatg gcattctcct gccaggcttc aagctgacct
      721 tttgtcgccc cactcaaatc agcggtggcg ataatgagct gctcacgaac aaactgtaaa
      781 tactggttat tcatgcacac tccagctctg tgatttttat ccccaaccga ccaccaggaa
      841 caggcagtcc gcgcacaata ttggtttcat caaactgctc gtcgtcgata agtgaggtgt
      901 actggcaata gcggacacta ccatttgttc tttttttaag cagccatctg atgat
//
LOCUS       DADMGC010000343          954 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.351, whole genome shotgun sequence.
ACCESSION   DADMGC010000343 DADMGC010000000
VERSION     DADMGC010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.351"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..>954
                     /locus_tag="JNZ73_004378"
     CDS             <1..>954
                     /locus_tag="JNZ73_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY8734372.1"
                     /translation="ACGTRILGVKEYICDKPECPHVRYVTNSCGSRACPSCGKKATDL
                     WIATQLNRLPDCDWVHLVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGQEP
                     GIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLK
                     AWSEGLAMPESLSHITTESQRRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKP
                     PIAASRLAHYNGGASLSFRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFG
                     FLASRVCGEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSR"
BASE COUNT          223 a          239 c          309 g          183 t
ORIGIN      
        1 ggcctgcggc acgcggatac tgggcgtgaa ggaatacatc tgcgataaac ctgagtgccc
       61 ccacgtcaga tacgtcacta actcatgcgg cagccgtgcc tgcccgtcct gcggaaaaaa
      121 ggccacagac ctgtggatag cgacacagct gaatcgtctt cctgactgcg actgggtaca
      181 cctggtcttc accctgccgg acacgctgtg gccggtgttc gaaagcaacc ggtggctgct
      241 gaatgacgtg tgccgtctgg cggtggagaa tctgctgtat gccgcccgga aacgggggca
      301 ggaacccggt atcttctgcg ccatccacac gtatggccgt cgtctcaact ggcatccgca
      361 tgtacatgtg tctgtaacct gtggaggtct gaataagcat ggtcagtgga aaaagctgag
      421 cttcctgaaa gacgcgatgc gttcgcggtg gatgtggaat atgcggcaac ggcttctgaa
      481 agcgtggtca gaggggctgg cgatgccgga gtcgttgtca catatcacga cggaatcaca
      541 gaggagaagc ctggtgctga aagccggagg aaaatactgg catgtgtaca tgtcgaaaaa
      601 aacggccggc gggaggaata cggcgcgcta cctgggtcgt tatctgaaga agccgccgat
      661 agcggcctcc cggctggcac attacaacgg aggggcgagc ctgagcttcc gttacctgga
      721 ccacaaaacg ggagaaacgg cgacggaaac gctgacacag cgtgagctgg tcgcgaggct
      781 gaaacagcac atcccggaga agttttttaa gatggtgagg tacttcgggt tccttgccag
      841 ccgggtgtgt ggagagaagc tgccgcaggt gtaccgtgct ctggggatgg ataaaccgga
      901 accagtggcg aaagtgtgct atgcacaaat ggtgaaacag ttcctgagtc gtga
//
LOCUS       DADMGC010000344          931 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.352, whole genome shotgun sequence.
ACCESSION   DADMGC010000344 DADMGC010000000
VERSION     DADMGC010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.352"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..88
                     /locus_tag="JNZ73_004379"
     CDS             <1..88
                     /locus_tag="JNZ73_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734373.1"
                     /translation="LASNGSTLTIQSNGAAFTATTIKIIAEQ"
     gene            88..543
                     /locus_tag="JNZ73_004380"
     CDS             88..543
                     /locus_tag="JNZ73_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HAY8734374.1"
                     /translation="MIQFKPTRNIDLIEAVGNHTDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            546..>931
                     /locus_tag="JNZ73_004381"
     CDS             546..>931
                     /locus_tag="JNZ73_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HAY8734375.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPT"
BASE COUNT          259 a          230 c          239 g          203 t
ORIGIN      
        1 tcttgcctca aatggcagta cgttaactat tcagtcaaat ggtgcggcgt ttactgccac
       61 aaccatcaag ataatcgcgg agcagtgatg attcagttca aaccaacgcg aaacatcgac
      121 ctgatcgaag cagtaggaaa tcacactgac attatcgccg ggagcaacaa cggcgatgga
      181 tacgactaca agcctgaatg ccgttacttt gaggttaacg tgcacgggca gttcggcgga
      241 attgtttact atcaggagat tcagccgcta acctttgatt gccacgccat gtacctgcca
      301 gagattcgtg gattcagcaa ggaaatcggg ctggcgttct ggcgatacat tctgactaac
      361 accaccgttc agtgcgtcac atcgttcgct gcgcgcaaat tccgccacgg tcagatgtac
      421 tgcgcaatga ttggccttaa gcgtgtagga accatcaaga aatacttcaa aggcgtggat
      481 gacgtgacgt tttacagcgc cacacgcgaa gaactaatcg acttcctgaa tcacgggaga
      541 tagccatgtt atatgcattt aagctgggca gaaaactgcg cggcgaggaa ccttattgcc
      601 ctgaaaaagg cgggaaaggt ggcagctctg ataaaagcgc aaagtatgca gcagaagctc
      661 agaagtatgc cgcagacctg caaaatcagc agtggcagac gatcatgaaa aaccttgctc
      721 cgttcacgcc tcttgcggag cagtatgtta accagcttca gaacctttcc agtttagaag
      781 gtcaggggca ggcacttaat cagtattaca actctcagca gtataaagac cttgcaggtc
      841 aggctcgtta ccagagtctt gctgctgcgg aggcgacggg tggacttggt tcgacagcca
      901 caagcaatca actggctacg atcgcgccga c
//
LOCUS       DADMGC010000345          929 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.353, whole genome shotgun sequence.
ACCESSION   DADMGC010000345 DADMGC010000000
VERSION     DADMGC010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.353"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..256
                     /locus_tag="JNZ73_004382"
     CDS             <1..256
                     /locus_tag="JNZ73_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734376.1"
                     /translation="WFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVR
                     KASWHCRQCHHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            244..678
                     /locus_tag="JNZ73_004383"
     CDS             244..678
                     /locus_tag="JNZ73_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734377.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
BASE COUNT          214 a          214 c          283 g          218 t
ORIGIN      
        1 ctggtttgaa cacactgacg acaggctgac agagcacggt caacagtgcc cttatgtcag
       61 gccggagcgc agagaaatac agttgattaa acgtctgcag caattcgtac cggatgcctt
      121 acccgtggtg cgtaaagcca gctggcactg cagacaatgt caccacgatt attatgggga
      181 gcagtactgc acacactgcc agaccggagg attcagtatt ccccggacaa ctcaggagga
      241 aatatgcgaa ttctgaactg ttatatggcg aatgacagca aaggccattt tgttacggcg
      301 aaagaagctg cgaagcacaa ccgacaggac gttttgtgct gtgtgtcctg tggatgcccg
      361 ttaacacttc agcggggcaa tgacggacaa ccaccgtggt ttgaacatga ccagatgact
      421 gtcgctgaaa aaatcctgct gcgatgtacc tggcttgacc cggcagagaa agaggcccgt
      481 cgtttgcatc tgcagggcat gacggttccg gattatacgg tgaaggtgag aaagtggttt
      541 tgtgtgatgt gtgacgaaga ttatgagggg gaaaagtgct gcccacgctg cggtaccggg
      601 gtatacagca gggcgtgggg gcggcaggag gtgccgtcgg aagatgccag ggctgataat
      661 ccgttacaga ggctgtaatg gttgcctccg gagcagtttg cggtgatgcc tttcccggga
      721 atggattgcc agtgcgggga ctgtggagct taattccggt gttggtggcc tttccgttga
      781 ttttatgcca acagccccct gtgactgaca ggctgcctcg tcattccatt cgtatctcca
      841 gtaacagggg gttgtattgt attttattgt gccggttcag gtgtgcgttt cccggcgcgt
      901 ctgcaccggc ttaaccaaat tcaacaggg
//
LOCUS       DADMGC010000346          895 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.354, whole genome shotgun sequence.
ACCESSION   DADMGC010000346 DADMGC010000000
VERSION     DADMGC010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.354"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(110..439)
                     /locus_tag="JNZ73_004384"
     CDS             complement(110..439)
                     /locus_tag="JNZ73_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HAY8734378.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(611..895)
                     /locus_tag="JNZ73_004385"
                     /pseudo
     CDS             complement(611..895)
                     /locus_tag="JNZ73_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          219 a          207 c          199 g          270 t
ORIGIN      
        1 tgttgttaat ttcgattggt tctcagatga gaatgatgac ggtgatacac agataagaat
       61 caatgagcaa tgagggttgc cgggcaaccc tcattgaata aaacgggaat tacttcgctt
      121 cgccgttttt catttcgccc atagctttca gctttttgga gatatcgcgg cgttctttgg
      181 agagctcggc atttttgatg atgtaatcgt caacgcgatc ttcatagtca cctttcatgc
      241 tggcgatgat gccctggatt gcttcaacgc tcatccctgg cttgatgtaa tcgctcaggt
      301 tgtccagcaa caggacgcgc ttctggttgt cacggatctt tttctcaacg tcctgaattt
      361 cacgttgcag tttgttctta cgacggaaca gacgaacaaa ttccagtacg tcctggaatg
      421 aaggcttggt agtttccatt tttatacccc tgataatgtg agagtcggat tcgtttaagc
      481 aaccgctatt cgttagggcc aacattactc atagctgccg cagatgacaa tgcttttatc
      541 ccttttcact atcataccct ttatccttgc tgaatcgaag cagcagcaag atgattctga
      601 agttcaggaa ttatttgcgc aaggcccggc aaatcagatt cgatagcaac tcaagctgat
      661 gtgccgtttc catgttcagc cacacataac catagattgg tgtttccacg actttcaggt
      721 catggtggtt atcgagcaac tgacgcagct cttccacagc atcgttcaat ttttccgtat
      781 tggcaaaaac cggctgcgga ttaccttcgt acagcgcatg aacaaggctc agcagtattt
      841 gctgcatcat gtgctgggta tcacgaagtt tttacgcgtt caataacacg aaatg
//
LOCUS       DADMGC010000347          878 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.355, whole genome shotgun sequence.
ACCESSION   DADMGC010000347 DADMGC010000000
VERSION     DADMGC010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.355"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..828
                     /locus_tag="JNZ73_004386"
     CDS             <1..828
                     /locus_tag="JNZ73_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAY8734379.1"
                     /translation="CSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMW
                     QARFYQALPKIVVDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPV
                     QGWIRATEVTRGKDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPN
                     IDIRAVKTKTGEVVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFI
                     STGGALKNVLQLDRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
BASE COUNT          225 a          183 c          253 g          217 t
ORIGIN      
        1 tgtagcggtt tgttacgttt tggttggtct accatcatgg aaaccggaga aacgcgccta
       61 cggcttcgta gtgcgcaatt ttgccgtgtc cgtcattgcc ctgtctgcca gtggagaaga
      121 accctcatgt ggcaagcccg tttttatcag gctctaccga aaatcgttgt ggattacccg
      181 tcttcccgat ggttgtttct gacgttaact gtcaggaact gcgagatagg tgaacttgga
      241 acagtcctta cagcaatgaa tgcggcgttt aagcgaatgg aaaagcgaaa ggagctatca
      301 cctgttcagg ggtggatcag ggctacggag gtgacgcgag gtaaggatgg cagcgcacat
      361 ccgcattttc actgtctgct gatggtgcaa ccttcttggt ttaaagggaa gaactacgtt
      421 aagcacgaac gttgggtaga actctggcgc gattgcttgc gggtgaacta tgagccgaat
      481 atcgatattc gggcagtaaa aactaagaca ggtgaggttg tggccaacgt tgccgagcaa
      541 ctgcaaagcg cggttgctga aacgctgaaa tactccgtta aaccggaaga tatggcaaac
      601 gatcctgagt ggtttcttga gctgacgcgg cagcttcaca agcgccgttt tatctcgacc
      661 ggtggggcgc taaaaaacgt cctccagttg gatcgagaaa ccaatgagga tcttgtcatt
      721 gccgacgatg taggggatgg cactgatgac gggaagcgga cggcgtttgt ctgggattca
      781 ggtaaacggc gttacaaacg cgcccctgag aaggataaat cggattaacg tatgaatatt
      841 aatattgaat acctgaatgg aaataagact attggttt
//
LOCUS       DADMGC010000348          878 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.356, whole genome shotgun sequence.
ACCESSION   DADMGC010000348 DADMGC010000000
VERSION     DADMGC010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.356"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(477..734)
                     /locus_tag="JNZ73_004387"
     CDS             complement(477..734)
                     /locus_tag="JNZ73_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734380.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
BASE COUNT          230 a          207 c          212 g          229 t
ORIGIN      
        1 ttttccggac tctgaaagat taccaatact catcgtccat ccgcttgctt taggctgaca
       61 ggttcataat caacgcaaac cagagctgta caggcttggg cgcggctttc aaaccagtcg
      121 tgatcacggc aatcaatttt gaactctgct taacggacat ttctgtataa cccttacggc
      181 aacgaaaaac gcgaagttaa aattttagaa acccaaaaac gtgacatgac taagtttaga
      241 tttcaggggg ggagatcaaa aaatttcgct ctgtgccaga gcggacattc acggagctgg
      301 ttcattacca atgaggttgg gcttttgagg ataaatcaat gatcagacgc caacgtaaat
      361 caaaagcaac cctggaacgg aattgctaat ccagtttctg accatcgatt tttctaaaaa
      421 gtgtgcgttt gctactactt aggtaggtgc agctttctta atcaccggca gccacgttat
      481 acaggccagt tgatggatcg attgttatca atgatatctt catgagtcgg tgtctcaccc
      541 agcttaccga agctggcata aagtgaaggc agacgggccc gtccttctcc ctttttcgcc
      601 agagggaaag cgcgaagcat ggtggcagcc tcccagtcac aacaatagga tggtgtgcac
      661 ggctgctgac gccatgattc agcgatagag ccggaaaata cggggtcaaa gccggtatca
      721 ttaaccagag tcatcgtgac ctgtactgcg gctggatgat ctcctgctac tgcaactgct
      781 aggcgaccgc ggctcccttc aggtagtctg ttgtggtcaa ctaaaactgg cctccgcgtt
      841 agagtttttc cagtatcggt tttctgattc gtttggtg
//
LOCUS       DADMGC010000349          874 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.357, whole genome shotgun sequence.
ACCESSION   DADMGC010000349 DADMGC010000000
VERSION     DADMGC010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.357"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..202)
                     /locus_tag="JNZ73_004388"
     CDS             complement(<1..202)
                     /locus_tag="JNZ73_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAY8734381.1"
                     /translation="MNAHWSSKKSNFFRKNIKLLTKYLFFESQGIPDKVDIVSRLKTY
                     GYSISGVETDDGYKALVRAFQLH"
     gene            complement(171..422)
                     /locus_tag="JNZ73_004389"
     CDS             complement(171..422)
                     /locus_tag="JNZ73_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8734382.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGTFSYIFGSIVATLGMG
                     LVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLEFEKE"
BASE COUNT          247 a          230 c          192 g          205 t
ORIGIN      
        1 aatgaagctg aaaagcgcgg acaagcgcct tatatccgtc atccgtctcc acaccagaaa
       61 tactgtatcc ataggtcttc agccgtgaca cgatatcaac cttatccgga atgccctgcg
      121 attcaaaaaa taaatattta gtcagtaatt ttatgttttt cctgaaaaaa ttactctttt
      181 tcgaactcca gtgagcgttc acgactcatc agtgtgaaaa tgatggcaaa aacagcagac
      241 agtatcagta cgataaatgt ggccccccat cccagataat cgacaacaag tcccattccc
      301 agtgtcgcaa caattgagcc gaagatatag gaaaatgtgc ctgacagccc taacagaata
      361 agtttacgcg taaatcgccc ggaatccaga agagcattaa gtatcacata aacaggttcc
      421 ataccagtcc tgaccgcatc aggacgccag taaataaaat ctcgtagtcc accggcatca
      481 gagacaagag taatcgtcgt taccggtatt tcgactggtt tgtactggtg tgagcggaca
      541 aaactactgt acgtctctca tacacgctcg taagcggccc tcacggcccg ctaacgcgga
      601 gatacgcccc gactgcgggt aaacccttgt cgggaccact ccgaccgcgc acagaggcat
      661 tttcatcgct gacaggccgc atggcctcgc agagccggag ggggactggt gatattcgga
      721 cgaccgtacc ggcttgcgcc gggcttcggc ggttttacac ctgtgctaca tgtaaccacc
      781 gtatgccgcc ttatatgcgc tgactcgcat tatttgcctt gttccgtctg aaagtaatca
      841 ctatgattaa atatgattaa caactaatcg ggta
//
LOCUS       DADMGC010000350          871 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.358, whole genome shotgun sequence.
ACCESSION   DADMGC010000350 DADMGC010000000
VERSION     DADMGC010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.358"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..599
                     /gene="ospC4"
                     /locus_tag="JNZ73_004390"
     CDS             <1..599
                     /gene="ospC4"
                     /locus_tag="JNZ73_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HAY8734383.1"
                     /translation="LHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEYHIP
                     RMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADIALY
                     LLSKFNFTKQDVAEMEKMNNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNKKF
                     QKANSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          306 a          135 c          170 g          260 t
ORIGIN      
        1 ggttgcatct tattgatttt ataagaaaga gtaaagatca aggattcaga gagttttgtt
       61 ataacaaaaa tattgatcct gttagtctgg atagaattat aaactttgtg tttcagcttg
      121 agtatcatat accaagaatg ctaagtacag ataacttcaa aaaaataaaa ctcagagaca
      181 tatctttgga ggatgcgatt aaagcatcta attatgaaga gattaacaat aaagtgactg
      241 ataaaaaaat ggctcatcag gcccttgctt attctttagg caataaaaaa gctgatatag
      301 cactttattt actatctaaa tttaatttta caaaacaaga tgtcgcagag atggagaaaa
      361 tgaacaacaa tagatattgt aatctgtatg atgttgaata cttacttagt aaagatggtg
      421 ccaactataa agtgctagaa tattttatca ataatggatt ggttgatgta aacaaaaaat
      481 ttcaaaaagc aaatagtggg gacactatgc ttgataatgc aatgaaaagc aaagattcaa
      541 aaatgattga ttttttatta aaaaatggag caatattagg caaacgattt gagatatgac
      601 ctaaaagctc ggccaaataa taatgtcgtc gattatcgaa gtcatcatga gtgattctgt
      661 ccccacagtg cagcatcagc atgacagtga attgtctggc gtggtccatt taatgagatg
      721 gagatgctcc ctctttccta aatagctgtg cctaataccg ctacactttt gccagcccat
      781 gtttgcctcc ggggaatggg ctgacggttc cttcaaaatc cattgcacgc agttatgtcg
      841 gagcgggaac ttgactcctg tctttacggg a
//
LOCUS       DADMGC010000351          867 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.359, whole genome shotgun sequence.
ACCESSION   DADMGC010000351 DADMGC010000000
VERSION     DADMGC010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.359"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..>867)
                     /locus_tag="JNZ73_004391"
     CDS             complement(<1..>867)
                     /locus_tag="JNZ73_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY8734384.1"
                     /translation="VIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQRFTQPY
                     ASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQAAENYGS
                     AALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSLRLVGYR
                     YSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLSRQQYWH
                     TDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLSLSVPLS
                     GWWGNSYATYSVS"
BASE COUNT          227 a          223 c          197 g          220 t
ORIGIN      
        1 cgaaacagaa taggtggcgt aactattacc ccaccagccc gataaaggaa cagataaaga
       61 gagcatgtag atctgctcac tggtgttatc actggcatgg ttgttccccg tcccctgtct
      121 ggcagtattg gaataactcc aggagacgtt ccacgagaga cgcttccagc tactgctgta
      181 gccaaattgc attaatcgtt cgacatcatc ggtatgccag tattgttgcc ggcttaatgt
      241 caggtataaa ttgccataat ctctccccaa cgactgcgtt agcgttccct ccacgcgact
      301 acgtcggtta cctgtttccc aaaagtcttc agggctgttg cgccgcgatg cccactcatt
      361 gagggtatag tagccctcgg tggagtaacg atagccaacc aggcgcaagc tagtgtctgt
      421 gtcgtcaaat agttttgaat agagaaagcg atatgactga cccgtttctg tatcatcatg
      481 gctaaaattc gcacgagcat gtgtcacatc gaaagaaatt gcgcccaaag cgccaagatc
      541 tttaccgaca cccagagctg cagaaccata attttcagca gcctgcatac ctccatataa
      601 agtgatcccg tgggaaaaac catgaagtat ttgcgcctga aggacgtccg gtgtaaatcc
      661 gtcttcatcg cgcaattcac ccacgctgac atcaacatct gtcagacctt cacgtttaag
      721 aatcgccaat gaagcataag gttgggtgaa tcgttgttcg ctaccgtctt cttctttgat
      781 ggttacgtcc aaatcgccac ctgtacttgc ggtgttgaga tcggtgatct caaaagcacc
      841 ttgtggaacg gctgactgat aaatcac
//
LOCUS       DADMGC010000352          853 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.360, whole genome shotgun sequence.
ACCESSION   DADMGC010000352 DADMGC010000000
VERSION     DADMGC010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.360"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            1..761
                     /locus_tag="JNZ73_004392"
                     /pseudo
     CDS             1..761
                     /locus_tag="JNZ73_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          231 a          185 c          202 g          235 t
ORIGIN      
        1 agcgcattct gaccgaccgt gaaaatggtg aaaaaggcga taattctcaa gatacctctt
       61 atgcccgtgt aggcgtgaaa ggtgaaactc agatcaaccc tgaaatgact ggttacggtc
      121 agtttgaact agacctggaa gcatcaaaca gacacaaccc tgaccaaact cgtttggcat
      181 atgctggttt gagttacaaa gattttggtt cattcgacta cagtcgtaat gtcggtgtcg
      241 cgtatgatgc agaagcattt actgatatgt ttgttgagtg gggcgctgac tcatgggctg
      301 gtactgacct gttcatgacc aaccgtacca acggtgttgc aacctatcgt aacacagatt
      361 tctttggcat ggttgaaggt ctaaattttg ctctgcaata tcagggtaaa aatgaaggta
      421 caggcaacta caaagcaaat ggcgatggcc atggtttatc tgcaacttac actattgacg
      481 gtttctcatt tgctggtgct tatgcaaact ctgaccgtac tgattggcag tctggtgatg
      541 gtaaaggcga aagagccgaa gtttgggcgt tgtcaacaaa atatgacgct aacaatgttt
      601 atgctgctgt gatgtacggt gaatctcaca acatgaattc cgacgacggt gatgttgtga
      661 acaaaacaca aaacttcgaa gctgttctgc aatatcagtt cgactttggt ctgcgtccat
      721 ccatcggtta ctcctattct aaagcactgg acgtagcagg ttggatttgc ccctatattt
      781 ccagacacct gttatcactt aacccattac tggcccgctg ccgtagatat tcccgtggcg
      841 agcgataacc cag
//
LOCUS       DADMGC010000353          846 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.361, whole genome shotgun sequence.
ACCESSION   DADMGC010000353 DADMGC010000000
VERSION     DADMGC010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.361"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..476)
                     /locus_tag="JNZ73_004393"
     CDS             complement(<1..476)
                     /locus_tag="JNZ73_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8734385.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLA"
BASE COUNT          268 a          163 c          180 g          235 t
ORIGIN      
        1 tgcgcaagat ttaagcggaa gtgactactg ctttccggcc ctaataccgt aacaaaaggg
       61 tttgcggcag tttccagaca acctaatccg gctgcaataa taaataggcc aactaaaaat
      121 aaggtgtagt tcattatttc tgcagcgggc cagaataatg cagcgcccaa ggcatataaa
      181 aataatccgg taataatccc tgctttataa ctgagttttt tcatcaatat cccagcaggg
      241 attgggataa tgaaataacc aaagtaaaag gccgattgga tcaggccagc ctggaaattt
      301 gtcagcgtaa aagcctgctg gaattgaggt aataaaatgt cgttaaggtt attggctacc
      361 gcccaaagaa aaaacagtga gcacagcagc gcgaatggaa taatgtaact tctgctttgc
      421 cctgcatctt tatctaccgc acggtaactc tgcgtttgta ttgatgtgtt tcccatagca
      481 tcctcttagg ttcagaagct taagcacgta atcggttatg ttttcaaaag tgcaaaacca
      541 gcgagttcgg gcttcatatc ggtgaatatt tgcctgtctg aaatgaactc atggattcat
      601 tttcaaataa aaaacgctct gtaatatgac ggcggtcaca cttattagct ggaaggtgac
      661 tttatgtgac taccatcact ttaataatcg aaatttcatg cccgtttcag aaaataaatc
      721 ataaaaaaca caaaccagat gaccgtatta attgtttgtg ccgaaaacgg gcatttctgg
      781 cgaggggaaa tggaaaaatg accgttttga taaatatttt taagagcggt catttttaac
      841 ggggtt
//
LOCUS       DADMGC010000354          834 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.362, whole genome shotgun sequence.
ACCESSION   DADMGC010000354 DADMGC010000000
VERSION     DADMGC010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.362"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..161)
                     /locus_tag="JNZ73_004394"
     CDS             complement(<1..161)
                     /locus_tag="JNZ73_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY8734386.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIA"
     gene            complement(161..760)
                     /locus_tag="JNZ73_004395"
     CDS             complement(161..760)
                     /locus_tag="JNZ73_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY8734387.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          203 a          221 c          199 g          211 t
ORIGIN      
        1 caggcaatcg ctgcctgcat atcgtgtggt tttgtcgctg ttccgcacgt ccccgccagc
       61 ctgtaatgcg ccagcacaga agtttcggga ttgtgattgc agtagccagg aattctgacg
      121 gtgcacatct gcccccgcgc cgctttacgt aaatccacca ttacgcaaac tccagtaact
      181 gcgcggccac attttcgact tcctccggag aggaaaattt acggaacagg atccagttcc
      241 acagcacatt cagtacagat ttataaacct gctgaaactc ggtttcgtcc atgttcgcaa
      301 atgcgataga ttttgccctg cgcccacgac taccatcagg ataaatatgc tcggtgtaaa
      361 atccggcctg aatggttacc cactcgcgga aagcgtcaaa cgactttagc aatgccgtat
      421 cccgggttct gcatgtcgca actgtattaa ggtattgctc tgcggcatca ctcagggctg
      481 gagtgtgttc ccgacctact gattcgcaca ggtaatcaac gaagcctgat accagttttc
      541 gttcgcgagg cgtgatcgcc ccaccgaccg gagtccagta atcaaatccc agttgcagga
      601 gtttgaaaaa acgcttgtgg aatgcgtagt tacgcacacg cttaaagtct gcgtgtatcc
      661 actcacctat tttgatttga tgcaaaaaat cgcaactctc cggcgtcgcc gggagaagta
      721 atccggaaga ggtttgtttg accagttgta tatgcgccat cgtagttctc cgctggcgca
      781 gtagaatggg tgttcagccc gttatgtagt ataccagaat taatgccaat acta
//
LOCUS       DADMGC010000355          830 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.363, whole genome shotgun sequence.
ACCESSION   DADMGC010000355 DADMGC010000000
VERSION     DADMGC010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.363"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..326
                     /locus_tag="JNZ73_004396"
     CDS             <1..326
                     /locus_tag="JNZ73_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HAY8734388.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="JNZ73_004397"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="JNZ73_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HAY8734389.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRLVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          255 a          181 c          153 g          241 t
ORIGIN      
        1 ttagtgagac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca ggttctatgt tcttcatcca tgttaccagt aacggccaga
      481 atatcgacca agcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgatccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag aaaaatgtaa
      781 gaagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga
//
LOCUS       DADMGC010000356          829 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.364, whole genome shotgun sequence.
ACCESSION   DADMGC010000356 DADMGC010000000
VERSION     DADMGC010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.364"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..>829)
                     /locus_tag="JNZ73_004398"
     CDS             complement(<1..>829)
                     /locus_tag="JNZ73_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY8734390.1"
                     /translation="VVTNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVV
                     EEIMARLAKEVRQAFSGVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFP
                     RFNSRIKRQSEQWQLVLLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVD
                     LTADVADPVELLMKVQLGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLT
                     HQVKKCVQSGIKVLGLAALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENL
                     "
BASE COUNT          190 a          225 c          217 g          197 t
ORIGIN      
        1 gaagattctc cgcaagccat gatgccagct cgcccggtgt catggcggct atttgtgcgc
       61 cgacattaac cagcgcctgg gccgtatcgc ggtcatagca aggtgttgct gtgctatcga
      121 gcgctgccag tcccagcact ttgatgccgc tctggacaca ctttttcact tggtgcgtca
      181 gcaatgatga tgaaccccct tcataaaaat cgctcacgag gataatgacg ctttttgcag
      241 gttgttcaat aagttgccga ccatactcca tggcactggc gatattggtt ccgccgccta
      301 attgcacttt cattaataac tctaccggat cggcaacgtc tgccgtgaga tcaacgacgc
      361 ttgtgtcaaa cgccaccaga tgggtacgaa tgccgggtaa ctgccataaa caggccgcca
      421 tcaccgcaga gtggatcacc gaatcgacca tcgatccgct ttgatcaacc agtaagacca
      481 gttgccattg ctcgctttgg cgtttaatgc ggctgttaaa gcgggggaat tcgatataca
      541 acttgccgtg ttgcgggtgc cagtgttgca ggttggcgcg cagagtactt ttgaaatcaa
      601 agtttcgcgc cagtggaata aatgagcggc gacgacgatc gcggacacca gaaaaagcct
      661 gacgaacttc ctttgccagt cgcgccataa tttcttccac aacctggcgc acaatcctgc
      721 gggcggcagc cagtacttcg gggttcatca gatgtttggt gtgcaaaacg gcgcgtagca
      781 ggctttcgga aggctgcata cgttccagca cgtcgagatt tgtcaccac
//
LOCUS       DADMGC010000357          828 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.365, whole genome shotgun sequence.
ACCESSION   DADMGC010000357 DADMGC010000000
VERSION     DADMGC010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.365"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            88..153
                     /locus_tag="JNZ73_004399"
                     /pseudo
     CDS             88..153
                     /locus_tag="JNZ73_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..629
                     /locus_tag="JNZ73_004400"
     CDS             381..629
                     /locus_tag="JNZ73_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY8734391.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(769..>828)
                     /locus_tag="JNZ73_004401"
     CDS             complement(769..>828)
                     /locus_tag="JNZ73_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY8734392.1"
                     /translation="KKYRVLLNRVDTSTAPDIE"
BASE COUNT          246 a          182 c          185 g          215 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacacgtca aatcggtaac ggctggaaat
      661 cattcaatac tcgcactatc gaaagtttac cagccaaccg cagcacgtcc tgcatacgtc
      721 gtgtctgcgg tttttctttt tcgcttacat tgtgtctggt tcttccggct actcaatatc
      781 aggtgcagtt gatgtatcaa cacggttcag caacacccga tacttctt
//
LOCUS       DADMGC010000358          801 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.366, whole genome shotgun sequence.
ACCESSION   DADMGC010000358 DADMGC010000000
VERSION     DADMGC010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.366"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..244)
                     /locus_tag="JNZ73_004402"
     CDS             complement(<1..244)
                     /locus_tag="JNZ73_004402"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY8734393.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQSATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
     gene            complement(241..663)
                     /locus_tag="JNZ73_004403"
     CDS             complement(241..663)
                     /locus_tag="JNZ73_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY8734394.1"
                     /translation="MWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTP
                     PEDSYYTPVVHGIAQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVR
                     ESNGEEQHFSVPFQTPAIALHEGYFKYSVMGGNIKKKV"
BASE COUNT          222 a          219 c          151 g          209 t
ORIGIN      
        1 attgagtacc aaaatcacta atggatttac tataacgaat acgatattta ttcccataat
       61 ataaattcgc aatcgaacgc gtaatatctg tcgataaact ccctaacagc cccagagaaa
      121 tgccaatacc cgtagatatt gcattaaaat gagtggcgct ttgaatccct ccatacaccg
      181 tgacgccata aggaagcccg tacatcgccg tgatttcgcc cacggagaca ggggagcttt
      241 ttataccttc ttctttatat tcccccccat cacactatat ttgaaatatc cttcatgcag
      301 agcgatggcc ggcgtttgaa atggcacgga aaaatgctgc tcctcgccat tactctctct
      361 cactgtaaca tcaagctccc cgccaacggc cagcgttggt aaattatcca gcgcaaaagg
      421 ccctggcggc acagaacggg taaaaataat ttgcccattt tgtttaataa taacctgcgc
      481 ctcactttgt gctattccat gcaccacagg tgtgtagtag ctgtcctccg gcggagtcat
      541 cgcatcgtcg gtcgctaaca tcccgccgcg aaaaggtatg ctgtcaaaca cctcagacga
      601 cgtataagat tctccaagcg ttaatcggct tttaatcgca tttatgcctc gctcaaacca
      661 catgtaaccc ctttgccatc tttctgactg gccaagttcc ttgctccatg tcactgaact
      721 acgaaacctc caggcaccaa gattaaaccc tggagtgaac ctgccataac gagaattacg
      781 cgtggtagat tgattatcat t
//
LOCUS       DADMGC010000359          794 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.368, whole genome shotgun sequence.
ACCESSION   DADMGC010000359 DADMGC010000000
VERSION     DADMGC010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.368"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            23..577
                     /locus_tag="JNZ73_004404"
     CDS             23..577
                     /locus_tag="JNZ73_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY8734395.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
BASE COUNT          199 a          171 c          232 g          192 t
ORIGIN      
        1 tctggagaaa ggaaggttat ttatgattta cccaacaaat acaggcaaaa gcggggaaca
       61 ccttcgtctc accacgctgg aaagtgtctg gattcaggga aaactgcgca tgtgggggcg
      121 ctggtcgtat attggcggcg gtaagacggg aaatatgttt aaccagttgc tggcatccaa
      181 aaaattgacg aaaacagccg tcaatgaagc cctgcgcagg atgaaaaaag cgggaataga
      241 gaaacctgag ctggaagcgt ttttgcgaga gatgatcaat ggcaagcaaa agacctggct
      301 ggcgcattgt actgatgcag aggcgttatg tattgataga gtcataagtg aggtgctggc
      361 agagcatcct ggattgatta gtgtcctccg gcaacggtat gaagggcggg ggatgactaa
      421 gcgtaaaatg gctgaattgc tgaatgacgc acaccctgag tggtgtttcc ggacgtgctg
      481 cagtcgggta gatgtatggc taaatcttgc tgaatatatg ctctatctac cgatgcgtga
      541 tgcattctct tccggggatc taaaaaccgt ttgttgactc aatctgttat ccggggctat
      601 attcctcacg cgcagcaaaa tctggcgtcg ggattggcgt cccggatgtt tacggagcga
      661 tatgagacgc gcccgcgtct tttttcatat cgtttgcaca gtcacattcg cgatttatgg
      721 cgggctgtgt gggggagccg aaaggctcgc cggtttccgt acccggttac gccaaccctg
      781 cacagttcgc cacc
//
LOCUS       DADMGC010000360          787 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.369, whole genome shotgun sequence.
ACCESSION   DADMGC010000360 DADMGC010000000
VERSION     DADMGC010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.369"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..200
                     /locus_tag="JNZ73_004405"
     CDS             <1..200
                     /locus_tag="JNZ73_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734396.1"
                     /translation="PTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLKL
                     DEGSNTASGLGKKYWTEICVR"
     gene            188..355
                     /locus_tag="JNZ73_004406"
     CDS             188..355
                     /locus_tag="JNZ73_004406"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY8734397.1"
                     /translation="MRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSVNPGLV
                     RASATFTLEFQ"
     gene            368..733
                     /locus_tag="JNZ73_004407"
                     /pseudo
     CDS             368..733
                     /locus_tag="JNZ73_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
BASE COUNT          224 a          167 c          193 g          203 t
ORIGIN      
        1 ttccaacgac cggtagcaca agtccagccg tcccttttca gattacgtta agtgaatgca
       61 gcaaagggac aacgggggtt cgggttgcat ttaacggtat tgaggatgca gaaaataata
      121 ctctgttgaa actggatgag ggaagcaata cggcctccgg tttgggtaag aaatactgga
      181 cggaaatatg cgtccggtga aactgaatga ccttcatgcc gggatgcagt ggatcccact
      241 ggtaccagaa cagaacaata ttttgcctta ctccgctcgt ctgaagtcaa ctcagaagtc
      301 cgtcaatccg ggactggtga gggcttcggc aacctttacc cttgaatttc aataatcaga
      361 gtcagatatg aaaagacttc acaagaggtt cctgttagct acgttttgcg cgttattcac
      421 agcaactctc caggccgccg atgtcactat cactgttaat ggtcgggtag tcgctaaacc
      481 ctgcactatt caaaccaaag aagctaacgt taatctcggg gatctttata cgcgcaatct
      541 gcaacaacct ggttctgcat ctggctggca caatattact ttgtcattaa ccgattgtcc
      601 ggttgaaaca agtgcagtga cggcaatcgt gacaggttca actgacaata cgggttatta
      661 caaaaatgaa ggtactgccg aaaatattca gatagagctg agggatgacc aggatgctgc
      721 gttaaaaaat ggagggtaat gctgccaact tactgattta gtgtatgatg gtgattttaa
      781 ggtgctt
//
LOCUS       DADMGC010000361          785 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.370, whole genome shotgun sequence.
ACCESSION   DADMGC010000361 DADMGC010000000
VERSION     DADMGC010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.370"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..241
                     /locus_tag="JNZ73_004408"
     CDS             <1..241
                     /locus_tag="JNZ73_004408"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734398.1"
                     /translation="DSFDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARC
                     TEREFATILAGISAGGMYCPECGTVHWPDGVAPPF"
     gene            241..>785
                     /locus_tag="JNZ73_004409"
     CDS             241..>785
                     /locus_tag="JNZ73_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8734399.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYA"
BASE COUNT          164 a          202 c          233 g          186 t
ORIGIN      
        1 tgatagtttt gacgatgccg ttgaatgctg cgcagacctg aatattccgt tctttgtgga
       61 tgcgggaaac aaaaagctgg tcttctggtt tgtacgtgtt gatgacgaag ggtatccgga
      121 aatcgcccgc tgcacggagc gggaatttgc gaccattctt gccggtatca gcgccggcgg
      181 tatgtactgc ccggagtgtg gcacggttca ctggccggac ggagtcgccc cgcccttctg
      241 atgcttcccc gttttgccga tatttttcag cagggaaacc gctggcttaa ctggctggag
      301 aaacaaccgg aaggggcagt ccgtccggtg gtaattgaat cagtgacaaa aatcatggcc
      361 tgcgggacca cactgatggg ctacacgcag tggtgttgtt catcaccgga ctgttgccat
      421 acaaaaaagg tctgcttccg gtgtaaaagc cgctcctgcc cgcactgcgg ggtgaaggct
      481 ggcgcacagt ggatacagta tctgctgagc ctggtccccg actgcccgtg gcagcatatt
      541 gtgttcacac ttccctgcca gtactggtcc ctggtgttcc acaaccggtg gttactggca
      601 gagatgagcc gcattgcagc ggatgtgata ctggaaatct gccatcaggc agatgtggag
      661 ccggggatat tcacggtgat ccacacatgg gggcgtgacc agcagtggca tccgcatatc
      721 catttatcga caactgccgg tggtgtgacg tcgggccaca cctggaaaaa tcttcatttt
      781 tacgc
//
LOCUS       DADMGC010000362          755 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.371, whole genome shotgun sequence.
ACCESSION   DADMGC010000362 DADMGC010000000
VERSION     DADMGC010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.371"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..148
                     /locus_tag="JNZ73_004410"
     CDS             <1..148
                     /locus_tag="JNZ73_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734400.1"
                     /translation="EQIAGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMM
                     GYGV"
     gene            complement(191..>755)
                     /gene="rtcA"
                     /locus_tag="JNZ73_004411"
     CDS             complement(191..>755)
                     /gene="rtcA"
                     /locus_tag="JNZ73_004411"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HAY8734401.1"
                     /translation="TTLLRHGFYPAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEV
                     LLAGVPRHVAEREIATLAGIFSLHEQNIHNLPRDQGPGNTVSLEVESENITERFFVVG
                     EKRVSAEVVAAQLVKEVKRYLASPAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLT
                     NIAVVERFLPVRFGLIETDGVTRVSIE"
BASE COUNT          181 a          230 c          187 g          157 t
ORIGIN      
        1 tgaacaaatt gcgggcgaac tggaagtggc ggcaaccacc atcaagacgc atatccgcaa
       61 tctgtaccaa aaactcggcg tcgcccaccg tcaggctgcc gtgcagcacg cgcagaaact
      121 gctgaagatg atgggttatg gggtgtgagt ttagccggat aatgcattgg atccggttta
      181 catctttaca tcattcaata ctcacccgcg ttacgccatc tgtttctatc aaaccaaacc
      241 gcaccggcaa gaaacgctcc accaccgcga tattggtcag cagatggcat gaaggatggg
      301 cgaccgtaaa ttctcccgcg cccgccagcg ccatcggcag taccagttgg tcggcgagat
      361 attcccccac cgccgctggg cttgccaggt agcgtttcac ctctttcact aactgcgcgg
      421 ctaccacctc ggcactgacg cgcttttcac cgacgacgaa aaagcgttca gtgatatttt
      481 cactttcgac ttccagcgag acggtattac ccggcccctg gtcgcgcggc aggttatgaa
      541 tattctgttc atgcagggaa aaaatccccg ccagtgtagc gatttcacgc tcagcaacat
      601 ggcgcggcac accagctaat agaacttctc cacgcatctg cacaatgttc ccgcgctcgc
      661 caagttgcaa ggtgctaaac gatgccacgg gcgagacttc cgttgccacc acaccgcctc
      721 cggcaggata aaaaccgtga cgtaacagcg tggtt
//
LOCUS       DADMGC010000363          743 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.372, whole genome shotgun sequence.
ACCESSION   DADMGC010000363 DADMGC010000000
VERSION     DADMGC010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.372"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..501
                     /locus_tag="JNZ73_004412"
     CDS             <1..501
                     /locus_tag="JNZ73_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="regulates the transcription of lsr genes; involved
                     in autoinducer 2 uptake, biofilm formation and stress
                     responses; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HAY8734402.1"
                     /translation="GVGSYMTGIGQLNAACSVNIIPAPLRASSADIARTLKNENCVKD
                     VLLAAQAADVAIVGIGAVSQQDDATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKG
                     DVVTDIKIHNELIGLPLSALKTIPVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTA
                     AAILRS"
BASE COUNT          197 a          162 c          213 g          171 t
ORIGIN      
        1 ggcgtcggtt cttatatgac gggtatcggg cagcttaacg cggcgtgcag tgtgaatatt
       61 attccggctc cgttgcgggc atcctccgct gacattgccc gtacgctaaa aaatgaaaat
      121 tgcgtcaaag atgttctgct agccgcgcaa gcagcggatg tggcgattgt cggcattgga
      181 gccgtgagtc aacaagacga cgcgacaatc attcgctccg gttatatcag ccagggcgaa
      241 cagttaatga ttggccgaaa aggggcggtt ggcgacattt taggctactt ttttgatgca
      301 aaaggtgacg ttgtcacgga tatcaaaata cataacgaac tgattggctt acctttaagc
      361 gcgctgaaga ccatacccgt ccgggttggc gtggcagggg gagaaaataa agccgaagca
      421 attgccgctg caatgaaagg cggttatatc aacgcactgg ttaccgatca ggacacagca
      481 gcggcgattt tacgtagtta aatttgcatg accttattac cgggaatgat ggagaacgta
      541 tagaggaaca gacagaacgc ataagccgag gataatctaa tggcttgact ctttacccct
      601 tcagaatcaa agtactacct gatggcgctg gatgcaggca ccggaagtat tcgggctgtg
      661 atattcgacc tggaaggcaa tcaaatagca gtgggacagg cggagtggcg gcatctggca
      721 gtaccggacg ttcctggttc tat
//
LOCUS       DADMGC010000364          742 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.373, whole genome shotgun sequence.
ACCESSION   DADMGC010000364 DADMGC010000000
VERSION     DADMGC010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.373"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..255
                     /locus_tag="JNZ73_004413"
     CDS             <1..255
                     /locus_tag="JNZ73_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY8734403.1"
                     /translation="NDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDK
                     EKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            279..>742
                     /locus_tag="JNZ73_004414"
     CDS             279..>742
                     /locus_tag="JNZ73_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8734404.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDIDAALTGT"
BASE COUNT          198 a          172 c          216 g          156 t
ORIGIN      
        1 aatgatgaat ttgtctttga ctacagactc aaagcggaag atgagcgtga tgcccttctg
       61 gctcaggtca gtgcccggac aggggaatgg gaagaagacc tgctgctggg gttaatcagc
      121 gacgaagata aagaaaagct gaaagcctgt cgtatttacg cgaaatcgct gcaggcgatg
      181 gatttcagca ccatcactga taaatcctca tacaacgcca ttgaatggcc cgcctctccg
      241 gaaggttctt cctgatttaa tttatcgcga gaaaaacaat gtctgtagtg atatcaggtg
      301 cgctgactga tggcgcaggt atccccatgt ccggatacca tattattctg aaatcccggg
      361 taaacacccc ggaagtggtg atgaacactg ttgctgatgt gatgacagga aacgatggtg
      421 aatactgttt ccatgcgcgg actggaaaat atggtgtgta tctgaaacag gactggcgca
      481 acgagtacaa cgttggcgac attgctgtat atgaggactc aaagcccggc acgctgaatg
      541 actttctgat tgctcctgat gagggcgacc tgaaaccgga tgtcgtcaaa cgctttgagg
      601 aaatggtggc gcaggcgcag cagagcgccg gggccgcagc cggaaacgca cagcagacgg
      661 cgcaggatgt ggcggcagcc gcaggttatg cccgcgcagc agagcaggcc aaaaatgaca
      721 ttgatgctgc gctgaccggc ac
//
LOCUS       DADMGC010000365          738 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.374, whole genome shotgun sequence.
ACCESSION   DADMGC010000365 DADMGC010000000
VERSION     DADMGC010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.374"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            15..488
                     /locus_tag="JNZ73_004415"
     CDS             15..488
                     /locus_tag="JNZ73_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8734405.1"
                     /translation="MAIAFKNIQAGTARKFTLSSANNSTKNAAFYLWGNPSRPVVAEL
                     GDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSP
                     GSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNVASI"
     gene            548..>738
                     /locus_tag="JNZ73_004416"
     CDS             548..>738
                     /locus_tag="JNZ73_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734406.1"
                     /translation="MTKRYSVTWLFSEDGKNWYEELKNFASDTIKIAYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDIT"
BASE COUNT          218 a          152 c          188 g          180 t
ORIGIN      
        1 gctttcagaa tggagtggct attgctttta agaatattca ggccggaact gccagaaaat
       61 tcacgttatc cagcgccaac aactccacga aaaatgcagc gttttatttg tggggtaatc
      121 catccaggcc tgttgttgca gagcttggtg atgattcagg ctggcatttt ttcagccaga
      181 gaaacccgga taacagcata gtgttcactg ttaacggaca ggtaattccg ttaaattacg
      241 gaaacttcga tgcccgctat aaatatcgaa cagagggggt acaggatgta cggtatggcc
      301 atgaaatgta ttacagcccc ggcagtaaca ccgtttcgtg gagattttgc gcaccttcgg
      361 gacacgggct gtcagggatg gcgatatcgg ataccggccg taactcagcg gataacgttg
      421 acggtgtgta ttaccgacca ctgcaaaaac tgattaatgg cacctggtat aacgtagcga
      481 gtatttaaca atgttgcatt taaaaaatat tactgcgggt aatccgaaaa cagcagaaca
      541 atatcagatg acaaaacgat attcggtcac ctggcttttt tcagaagacg gaaaaaactg
      601 gtatgaagag ctgaagaatt tcgccagcga cacaataaaa atagcttaca ccggagatgg
      661 tcgcgtggtg tgggtcggta aggatgtgac aggcatcgag ccacgcaatg ccagcgttat
      721 cgaagttccg gatattac
//
LOCUS       DADMGC010000366          719 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.375, whole genome shotgun sequence.
ACCESSION   DADMGC010000366 DADMGC010000000
VERSION     DADMGC010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.375"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..>719
                     /gene="wcaD"
                     /locus_tag="JNZ73_004417"
     CDS             <1..>719
                     /gene="wcaD"
                     /locus_tag="JNZ73_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HAY8734407.1"
                     /translation="AGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIRFKTI
                     SPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVLNFIK
                     FGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGVMTFI
                     LFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGSSSYY
                     RIVGPLVMVGYSLTHIDGVVR"
BASE COUNT          166 a          140 c          176 g          237 t
ORIGIN      
        1 gcgcgggact cacggcgttt aactacctgt ttggtcagtc gttggatgcc ggtaaatacg
       61 tcacttcaac tatgctgttt gtctatattg tgatcattat tggcatggtg tggagtattc
      121 gttttaaaac aatttcgcca cacaaccatc gtaagatatt acgtttcttt tatctggtgg
      181 tcggactggt ggtggcgctg gcggcggtgg agatggcgca aattatcctc accggtggca
      241 gcagtattat ggagtcgatt tcgaaatatc tgatttacag caacagctat gtgctgaatt
      301 tcattaaatt cggcggcaag cgcacgacag cactttattt cgaaccggca tttttcgctc
      361 tggcattaat ctcaatttgg ctcagcatca aacagtttgg tatcaaaacg cctaaaacag
      421 atgctatgat tctcgcaggg ataatattat ccggatcgtt ttcaggggtt atgaccttta
      481 tcctgtttta tttgctggag tgggcatttc aatatctgaa taaagaggcg attaagaaaa
      541 aattaccgtt agcattgatt tctctggctg tattcctggt tggtgtggta atcgcgtttc
      601 cttatatttc cacccgtctg ggcgatttag gtacggaagg atcgtcatcg tattatcgta
      661 ttgtcggtcc gctggtgatg gtcggttatt ctttgaccca tattgacggt gtagtcaga
//
LOCUS       DADMGC010000367          716 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.376, whole genome shotgun sequence.
ACCESSION   DADMGC010000367 DADMGC010000000
VERSION     DADMGC010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.376"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..>716
                     /locus_tag="JNZ73_004418"
     rRNA            <1..>716
                     /locus_tag="JNZ73_004418"
                     /product="23S ribosomal RNA"
BASE COUNT          205 a          151 c          221 g          139 t
ORIGIN      
        1 gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg aaggacgtgc
       61 taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga tttccgaatg
      121 gggaaaccca gtgtgattcg tcacactatc attaactgaa tccataggtt aatgaggcga
      181 accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga gattccccca
      241 gtagcggcga gcgaacgggg agcagcccag agcctgaatc agtgtgtgtg ttagtggaag
      301 cgtctggaaa ggcgtgcgat acagggtgac agccccgtac acaaaaatgc acatactgtg
      361 agctcgatga gtagggcggg acacgtggta tcctgtctga atatgggggg accatcctcc
      421 aaggctaaat actcctgact gaccgatagt gaaccagtac cgtgagggaa aggcgaaaag
      481 aaccccggcg aggggagtga aaaagaacct gaaaccgtgt acgtacaagc agtgggagca
      541 cgcttaggcg tgtgactgcg taccttttgt ataatgggtc agcgacttat attctgtagc
      601 aaggttaacc gaatagggga gccgaaggga aaccgagtct taactgggcg ttaagttgca
      661 gggtatagac ccgaaacccg gtgatctagc catgggcagg ttgaaggttg ggtaac
//
LOCUS       DADMGC010000368          712 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.377, whole genome shotgun sequence.
ACCESSION   DADMGC010000368 DADMGC010000000
VERSION     DADMGC010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.377"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            278..>712
                     /locus_tag="JNZ73_004419"
     CDS             278..>712
                     /locus_tag="JNZ73_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8734408.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLA"
BASE COUNT          217 a          123 c          147 g          225 t
ORIGIN      
        1 atgccaaata taaattttgt gtatcttttt ctgaaaataa actaaagaaa gggctttata
       61 tatcacgcat atttatttgt tggatagtca ttagatgttg aatcaatgaa ttactcgttc
      121 agttaatatt taaatattca tgaaatctat aaattaaaga tttgtcactt attggattta
      181 gtatgcttga tttaaatggc ggtcaattat tgttattatt aagtctaatg tcatttaaag
      241 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
      301 gtttataact gtggtatgtg caacgtccag cgttatggct gctgatgata atgctatcac
      361 ggatggctca gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      421 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      481 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      541 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      601 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      661 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cg
//
LOCUS       DADMGC010000369          693 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.378, whole genome shotgun sequence.
ACCESSION   DADMGC010000369 DADMGC010000000
VERSION     DADMGC010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.378"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..685
                     /locus_tag="JNZ73_004420"
     rRNA            <1..685
                     /locus_tag="JNZ73_004420"
                     /product="16S ribosomal RNA"
BASE COUNT          174 a          164 c          205 g          150 t
ORIGIN      
        1 ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg
       61 cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt
      121 cttgacatcc acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt
      181 gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc
      241 aacccttatc ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata
      301 aactggagga aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca
      361 cgtgctacaa tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa
      421 gtgcgtcgta gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta
      481 atcgtggatc agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
      541 accatgggag tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac
      601 tttgtgattc atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg
      661 gatcacctcc ttaccttaaa gaagcgttct ttg
//
LOCUS       DADMGC010000370          691 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.379, whole genome shotgun sequence.
ACCESSION   DADMGC010000370 DADMGC010000000
VERSION     DADMGC010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.379"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            337..684
                     /locus_tag="JNZ73_004421"
     CDS             337..684
                     /locus_tag="JNZ73_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY8734409.1"
                     /translation="MEEEIFTREEAASYLKVDKGTITQWIRSGRLQAVKINPDKPKSP
                     YRICKSDCIAALKSVRHNSAVNAVDVQEVKACQSNYAVARGTAISSRQMDQELDALLK
                     HRTKGKRKNFTIV"
BASE COUNT          216 a          160 c          168 g          147 t
ORIGIN      
        1 aatatctatg gtcatcactt cggcgctccc catgacgcag ccgatttcag acgatgcgtt
       61 gcacttgttg agcagatccc agaaatcaga gattcattcg acaaggttgc aaagcgcgtt
      121 ccggcattca aaggcatcct caacgaatgg gattccctcg ttgatctgtt gaagtctgaa
      181 atgaagatac acggaaacaa agcaccagag acttacagaa gaattagcga gttacgcaag
      241 gactaaccac agcctcatac tcgatgaggc ctgttcattg ctcaatgata tccagaccta
      301 ccgcaataat accaatccaa taaatggaga ttccaggtgg aagaagaaat cttcactcgt
      361 gaagaggcag cgtcgtatct gaaggtagac aaaggcacta tcacgcagtg gatacgaagt
      421 ggacgacttc aggccgtaaa gataaatcca gataaaccta aaagcccata tcgcatttgc
      481 aagtcagact gcattgcggc gcttaagtct gtgagacaca atagcgcggt gaatgcggtt
      541 gatgtgcagg aggttaaagc atgtcaatca aactacgcgg tggcacgtgg cactgcgatt
      601 tcgtcgcgcc agatggatca agagttagac gctctcttga aacatcggac aaaaggcaag
      661 cgcaagaact tcacgatcgt ctgaaagcag a
//
LOCUS       DADMGC010000371          683 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.380, whole genome shotgun sequence.
ACCESSION   DADMGC010000371 DADMGC010000000
VERSION     DADMGC010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.380"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            36..>683
                     /locus_tag="JNZ73_004422"
     CDS             36..>683
                     /locus_tag="JNZ73_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAY8734410.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHHNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMG"
BASE COUNT          160 a          172 c          182 g          169 t
ORIGIN      
        1 agggggtaat ctgctctcct gattcaggag agcttatggt cacttttgag acagttatgg
       61 aaattaaaat cctgcacaag cagggaatga gtagccgggc gattgccaga gaactggggc
      121 tctcccgcaa taccgttaaa cgttatttgc aggcaaaatc tgagccgcca aaatatacgc
      181 cgcgacctgc tgttgcttca ctcctggatg aataccggga ttatattcgt caacgcatcg
      241 ccgatgctca tccttacaaa atcccggcaa cggtaatcgc tcgcgagatc agagaccagg
      301 gatatcgtgg cggaatgacc attctcaggg cattcattcg ttctctctcg gttcctcagg
      361 agcaggagcc tgccgttcgg ttcgaaactg aacccggacg acagatgcag gttgactggg
      421 gcactatgcg taatggtcgc tcaccgcttc acgtgttcgt tgctgttccc ggatacagcc
      481 gaatgttgta catcgaattc actgacaata tgcgttatga cacgctggag acctgccatc
      541 ataatgcgtt ccgcttcttt ggtggtgtgc cgcgcgaagt gttgtatgac aatatgaaaa
      601 ctgtggttct gcaacgtgac gcatatcaga ccggtcagca ccggttccat ccttcgctgt
      661 ggcagttcgg caaggagatg ggc
//
LOCUS       DADMGC010000372          682 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.381, whole genome shotgun sequence.
ACCESSION   DADMGC010000372 DADMGC010000000
VERSION     DADMGC010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.381"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..556
                     /gene="sspA"
                     /locus_tag="JNZ73_004423"
     CDS             <1..556
                     /gene="sspA"
                     /locus_tag="JNZ73_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HAY8734411.1"
                     /translation="LAEKGVSFEIEHVEKDNPPQDLIDLNPNQSVPTLVDRELTLWES
                     RIIMEYLDERFPHPPLMPVYPVARGESRLYMHRIEKDWYTLINTIINGSASEADAARK
                     QLREELLAIAPVFGQKPYFLSDEFSLVDCYLAPLLWRLPQLGIEFSGPGAKELKGYMT
                     RVFERDSFLASLTEAEREMRLGRS"
     gene            562..>682
                     /locus_tag="JNZ73_004424"
     CDS             562..>682
                     /locus_tag="JNZ73_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein B"
                     /protein_id="HAY8734412.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQ"
BASE COUNT          147 a          178 c          191 g          166 t
ORIGIN      
        1 gctggctgag aaaggtgtaa gtttcgagat cgaacacgtg gaaaaggaca atccgcctca
       61 ggatctgatt gaccttaacc cgaatcagag cgttccgacc ctggtggatc gtgagctgac
      121 cctgtgggaa tctcgcatca ttatggaata tctggatgag cgtttcccgc atccgccact
      181 gatgcctgtt tacccggtag ctcgcggtga aagccgtctg tacatgcatc gcatcgaaaa
      241 agactggtac acgctgataa acactatcat caacggttca gcttctgaag cagatgccgc
      301 acgtaagcaa ctgcgcgaag aactgctggc gattgcgccg gtcttcggtc agaagccgta
      361 cttcctgagc gatgagttca gcctggtcga ttgctacctt gctccgctgc tgtggcgtct
      421 gccgcaactg ggcatcgagt tcagcggccc gggtgcgaaa gagctgaaag gctatatgac
      481 ccgcgtcttt gagcgtgact ctttccttgc ttctttaact gaagcagaac gtgaaatgcg
      541 tctgggccgg agttaatctg tatggatttg tcacagctaa caccacgtcg tccctatctg
      601 ctgcgtgcat tctatgagtg gttgctggat aaccagctca cgccgcactt ggtggtggat
      661 gtgacgctcc ctggcgtgca gg
//
LOCUS       DADMGC010000373          680 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.382, whole genome shotgun sequence.
ACCESSION   DADMGC010000373 DADMGC010000000
VERSION     DADMGC010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.382"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            1..219
                     /locus_tag="JNZ73_004425"
     CDS             1..219
                     /locus_tag="JNZ73_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY8734413.1"
                     /translation="MTGENRGRGRPRKTESTYADTRNDLIRSGLELLTQNGFLATGVD
                     AIVKNANVPKGSFYYYLKSKEDYAVNDG"
     gene            complement(270..659)
                     /locus_tag="JNZ73_004426"
     CDS             complement(270..659)
                     /locus_tag="JNZ73_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY8734414.1"
                     /translation="MRCSVDAYADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIG
                     YLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLDRILHHS
                     HIVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT          171 a          156 c          170 g          183 t
ORIGIN      
        1 atgacaggcg aaaatcgtgg ccggggaagg ccgcgaaaga cagaaagtac ctatgcagat
       61 actcgtaatg atctgatccg cagtggcctt gaactgctga cgcagaatgg ttttcttgcg
      121 acgggagtcg atgccattgt taaaaatgcc aatgtcccta aaggctcatt ttattactac
      181 cttaagagta aagaggatta tgctgtcaac gacggatgaa aagtgatcca cttatatctc
      241 caccaacggc ccaatattga tccaccgttt tactcaggat tagcttctgc tataaccccg
      301 gcctttcgtt tctgtctgag tcgatagctt tctcctttga tttgaacgat atgtgagtgg
      361 tgtaagatac ggtccagcat cgctgaggtc agtgctgcat caccggcgaa cgtttgatcc
      421 cactgcccga acggcagatt ggatgtcagg atcattgcgc tcttttcgta acgtttagcg
      481 atgacctgga agaacagctt tgcttcttcc tgactgaacg gcagatagcc tatttcatca
      541 atgatgagca ggcggggggc cattactcca cgctgaagcg tcgttttata acggccctga
      601 cgttgtgccg tagataactg aagtaacaga tctgcgtaag cgtcaacgga gcaccgtatt
      661 gacgcttatt tattggtgag
//
LOCUS       DADMGC010000374          663 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.383, whole genome shotgun sequence.
ACCESSION   DADMGC010000374 DADMGC010000000
VERSION     DADMGC010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.383"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..>663
                     /locus_tag="JNZ73_004427"
     rRNA            <1..>663
                     /locus_tag="JNZ73_004427"
                     /product="23S ribosomal RNA"
BASE COUNT          154 a          146 c          222 g          141 t
ORIGIN      
        1 tggtgggtag tttgactggg gcggtctcct cctaaagagt aacggaggag cacgaaggtt
       61 ggctaatcct ggtcggacat caggaggtta gtgcaatggc ataagccagc ttgactgcga
      121 gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa
      181 gggccatcgc tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag
      241 ttcatatcga cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag
      301 taggtcccaa gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg
      361 tcgtgagaca gttcggtccc tatctgccgt gggcgctgga gaactgaggg gggctgctcc
      421 tagtacgaga ggaccggagt ggacgcatca ctggtgttcg ggttgtcatg ccaatggcac
      481 tgcccggtag ctaaatgcgg aagagataag tgctgaaagc atctaagcac gaaacttgcc
      541 ccgagatgag ttctccctga ccctttaagg gtcctgaagg aacgttgaag acgacgacgt
      601 tgataggccg ggtgtgtaag cgcagcgatg cgttgagcta accggtacta atgaaccgtg
      661 agg
//
LOCUS       DADMGC010000375          661 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.384, whole genome shotgun sequence.
ACCESSION   DADMGC010000375 DADMGC010000000
VERSION     DADMGC010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.384"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..69
                     /locus_tag="JNZ73_004428"
     CDS             <1..69
                     /locus_tag="JNZ73_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8734415.1"
                     /translation="FYEINCPTVNNSCNVSVAKRDK"
     gene            71..>661
                     /locus_tag="JNZ73_004429"
     CDS             71..>661
                     /locus_tag="JNZ73_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734416.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEEGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIAPEFKGMSGAGA"
BASE COUNT          211 a          122 c          130 g          198 t
ORIGIN      
        1 ttttatgaaa taaactgccc gacggttaat aactcttgta atgtttccgt agccaaacga
       61 gataaataat atgcgctact tattaattgt aattatattt attataggtt ttagtgcatt
      121 acctgtatgg gcaatggact gctatgctga acatgaagag ggaaataccg tagtcattgg
      181 ttacgtacca agaatttcta tccccagtaa tggtaaaaaa ggtgataaga tctggcaaag
      241 cagtgagtat tttatgaatg tcttctgtaa taatgcatta cctgccccat cacctggaga
      301 agaataccca tcagcctgga ctaatataat gatgttttta ccaggcggtc aggactttta
      361 taatcagaac tcctatattt ttggcgtaac atacaatgga gttgattatg attcgactgc
      421 ccctaatgct atcgcagcac cagaatgcat tgatataaaa ggagcgggta cctttaataa
      481 tcactataaa aaccccgctg tctgtagtgg aggtcctgag cctcaattat cagtaacctt
      541 tccagctcgc gtacaactct atattaaatt agcaaaaaat gccaatagag ttaataaaaa
      601 cctggtgtta cctgatgaat atatcgctcc agagtttaaa ggtatgagtg gcgcaggagc
      661 t
//
LOCUS       DADMGC010000376          660 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.385, whole genome shotgun sequence.
ACCESSION   DADMGC010000376 DADMGC010000000
VERSION     DADMGC010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.385"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            104..>660
                     /locus_tag="JNZ73_004430"
     CDS             104..>660
                     /locus_tag="JNZ73_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY8734417.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLMVSGNRLDS"
BASE COUNT          236 a          121 c          111 g          192 t
ORIGIN      
        1 aagcaatacc tggagaaaga gtacaaagca aactgtatta aacttaataa gttatattgt
       61 atttcactag agttcttatc gttgataaga aattctggtt ataatgatta aatcaacaaa
      121 tatacaggta atcggttctg gtattatgca tcaaataaac aacatacact cgttaactct
      181 attttctttg cctgtgagtt tgagtccatc ttgtaatgaa tattatttaa aagtctggag
      241 tgaatgggaa aagaacggta ccccaggcga gcaacgcaat atcgccttca ataggctaaa
      301 aatatgtttg caaaatcaag gggcggaatt aaatctatct gagttagatt taaaaacatt
      361 acctgattta ccgcctcaga taacaacact ggaaataaga aaaaacctat taacatatct
      421 ccctgatttc ccaccaatgc ttaaggtaat acatgctcaa tttaatcaac tggaaagctt
      481 acctgccttg cccgagacgt tagaagagct taatgtgggt gataacaaga taaaagaatt
      541 accatatctt cctgaaactc taactcattt acggatccat aataaccgat tgcatattct
      601 gccactattg ccaccggagc taaaattact gatggtttct ggaaacagat tagacagcat
//
LOCUS       DADMGC010000377          656 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.386, whole genome shotgun sequence.
ACCESSION   DADMGC010000377 DADMGC010000000
VERSION     DADMGC010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.386"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..276
                     /locus_tag="JNZ73_004431"
     CDS             <1..276
                     /locus_tag="JNZ73_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY8734418.1"
                     /translation="LNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGSDNGGESAA
                     IIYSLLVTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
     gene            complement(521..>656)
                     /locus_tag="JNZ73_004432"
     CDS             complement(521..>656)
                     /locus_tag="JNZ73_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8734419.1"
                     /translation="VVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN
                     "
BASE COUNT          157 a          153 c          172 g          174 t
ORIGIN      
        1 ctgaacgtgt actgtcgtaa tggccaggtg gaaatcgaca acaacatcgg tgaaaacgcg
       61 ctgcgatcgg tggcggttgg aagaaaaaat tatctcttct tcggttctga caacggtgga
      121 gaaagcgcgg cgattatcta cagtctgctg gtcacctgca aactgaacgg agtggagccg
      181 gaggactggt tgcgcgaagt gatcgtgaag ctcaatgact ggccgtcgaa ccgagtgcat
      241 gaactgctgc cctggaactt ctcagctgta aaataatcct tccgctacgt acttctctgg
      301 gcgcttacca ctcaacggca tcgtcaaaat tatcaaagcg cagaacatag atttatcatg
      361 tgttttatgg atggctttct ccatcaggca tcgttcgtca cgggaaattg gtgctatgat
      421 cggcattgat cagtccggtt ggtgatttgt tttgatttgg cgattgatca gatcgcacaa
      481 tccgggctga gttccctcta agtgatctag gttgtgtccc ttaattacgt aagcgttgat
      541 aaaagagtcg gatgcagccc agtttcacca tcgccaggta atttctcgcc gttttgtcgt
      601 aacgcgtggc gatgcggcga tattctttca gccgcccaaa gcaccgttca acgaca
//
LOCUS       DADMGC010000378          656 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.387, whole genome shotgun sequence.
ACCESSION   DADMGC010000378 DADMGC010000000
VERSION     DADMGC010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.387"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(151..648)
                     /locus_tag="JNZ73_004433"
     CDS             complement(151..648)
                     /locus_tag="JNZ73_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY8734420.1"
                     /translation="MTTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDLKE
                     LRVALNYSQNPVSPKARSLILWTERGAARHAKMLETDQAWDVFEKLEDCYFSQRDPST
                     PVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKLVE
                     RLMQL"
BASE COUNT          173 a          159 c          146 g          178 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc ggaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttggcgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaa
//
LOCUS       DADMGC010000379          623 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.389, whole genome shotgun sequence.
ACCESSION   DADMGC010000379 DADMGC010000000
VERSION     DADMGC010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.389"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..>623
                     /locus_tag="JNZ73_004434"
     CDS             <1..>623
                     /locus_tag="JNZ73_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY8734421.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNELPASPVEWLTDN
                     GSCYRANETRQFARMLGLEPKSTAVRSPESNGIAESFVKTIKRDYIS"
BASE COUNT          148 a          152 c          196 g          127 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggatca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aacccgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgtg aggcactgca
      361 ctgggcggtc actacgggcg gcttcaacag tgaaacagta caggacgtca tgctgggagc
      421 ggtggaacgc cgcttcggca acgagcttcc ggcgtctcca gtagagtggc tgacggataa
      481 tggttcatgc taccgggcta atgaaacacg gcagtttgcc cggatgttgg ggcttgaacc
      541 gaagagcacg gcggtgcgga gtccggagag taacggcata gcagagagct tcgtgaaaac
      601 gataaagcgt gactacatca gtg
//
LOCUS       DADMGC010000380          617 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.390, whole genome shotgun sequence.
ACCESSION   DADMGC010000380 DADMGC010000000
VERSION     DADMGC010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.390"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..219
                     /locus_tag="JNZ73_004435"
     CDS             <1..219
                     /locus_tag="JNZ73_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8734422.1"
                     /translation="RKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEI
                     FYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          188 a          153 c          129 g          147 t
ORIGIN      
        1 cgtaaaggta actgttacga caatgctccg atggaaagct tctggggaac gctgaaaaat
       61 gagagcctga gccactatcg ttttaataac cgggatgaag ccatctcagt aatacgggaa
      121 tacattgaga ttttctacaa tcgtcagcgt cgtcactctc gtctggggaa tatctccccg
      181 gcagccttca gggaaaaata tcatcagatg gctgcttaaa aaaagaacaa atggtagtgt
      241 ccgctattgc cagtacacct caccattccc tcgcgatatt aacgtaatat cttaattgta
      301 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
      361 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
      421 gcaaggcgaa tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt
      481 aaaccgtctg gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga
      541 cactatcgta tggaataccg gacctgaatc actgaagctt tacgccttca tttcgaagaa
      601 catttaccac gggttgt
//
LOCUS       DADMGC010000381          613 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.391, whole genome shotgun sequence.
ACCESSION   DADMGC010000381 DADMGC010000000
VERSION     DADMGC010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.391"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(12..>613)
                     /gene="ospZ"
                     /locus_tag="JNZ73_004436"
     CDS             complement(12..>613)
                     /gene="ospZ"
                     /locus_tag="JNZ73_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HAY8734423.1"
                     /translation="HGYFNESPNIYDKKYISGITRSMAQLKIEEFINEKSRRLNYMKT
                     MYSPCPEDFQPISRDEASTPEGSWLTVISGKRPMGQFSVDSLYHPDLHALCELPEISC
                     KIFSKENSDFLYIVVVFRNDSPQGELRANRFIELYDIKREIMQVLRDESPELKSIKSE
                     IIIAREMGELFSYASEEIDSYIKQMNDRLSQIKARMPVT"
BASE COUNT          185 a          123 c           91 g          214 t
ORIGIN      
        1 attaatatac attaagtaac aggcattcga gccttgattt gagaaagcct gtcattcatt
       61 tgttttatgt agctatctat ttcctctgat gcataactaa aaagttctcc catttcacga
      121 gcaataataa tctcagattt aatagacttt aactctggcg actcatctct taacacttgc
      181 ataatctctc tttttatatc atatagttct ataaaccgat ttgcccgtag ttctccttgg
      241 ggagaatcat ttctgaatac aacaactata tacaaaaaat cactattttc tttagagaag
      301 atcttacaac taatctctgg gagctcacat aatgcatgta agtcaggatg gtacaaacta
      361 tccacagaaa attgccccat aggacgtttt ccagatataa ctgtcaacca gcttccttcc
      421 ggtgtactgg cttcgtctct ggaaataggc tgaaaatctt ccggacatgg agaatacatt
      481 gtcttcatat aattaagtcg tctggatttc tcattaataa actcctctat ttttaattgt
      541 gccatacttc tggttatacc agagatatac ttcttgtcat atatgtttgg tgattcatta
      601 aagtaaccat gtt
//
LOCUS       DADMGC010000382          612 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.392, whole genome shotgun sequence.
ACCESSION   DADMGC010000382 DADMGC010000000
VERSION     DADMGC010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.392"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
BASE COUNT          221 a          108 c          128 g          155 t
ORIGIN      
        1 gctgatgggg cgcttacatt aatcccgtac tctctggcca gttgggccac gcaggcccct
       61 agctggtaag atttttcaat aagatcaagc ttaaactcca tagagtcata gagtaagagg
      121 cacgaatcct gcgggggcga agagaatctg acattgatag agtccattaa cgttataaga
      181 ggactctatc atcgccaatc atcagacatt gaaatagacg acactgagat gacgcttaca
      241 attcttttgg tttctgtaat aagtaatctg acattccccg tgcccagaag aggagaggga
      301 tatcaagccc aagggttaat aattgatact acggttaaaa ttgccttcag taaaggaaac
      361 gtgattaaat tcaggggatt tcagagtgaa aaccatacaa catattaaaa tctgttcatt
      421 tagctataat acatacaaga aagtgtccag ttagataatg ttatctaaat aaccacagat
      481 aaaaacgcac ataattgggg gggggggatg aaaatacctg aagcagtaaa tcatattaat
      541 gttcagaata atattgatct tgttgatgga aaaacgaatc cgaataaagc aacaaaagca
      601 ttacaaaaaa at
//
LOCUS       DADMGC010000383          595 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.393, whole genome shotgun sequence.
ACCESSION   DADMGC010000383 DADMGC010000000
VERSION     DADMGC010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.393"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..449)
                     /locus_tag="JNZ73_004437"
     CDS             complement(<1..449)
                     /locus_tag="JNZ73_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY8734424.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSKLREAVATERLTVDQHRYINDVIGVLKACQDRLNG"
BASE COUNT          151 a          128 c          156 g          160 t
ORIGIN      
        1 ccattgagtc tatcctgaca ggccttaagc acaccgataa cgtcattaat atagcgatgc
       61 tggtctacag tcagacgctc ggtcgctaca gcttcccgaa gtttggactt aatgtcatcg
      121 tactggtcaa cgtaaagctg gtaggactcc gggtcgttca ggtcatcacg gctgacacta
      181 agttcccact cgacaatggt cttcaggtgc tcccgtagct cctcataaga ggcgttaggg
      241 ttgtctgcat ggattgcttt aagtgctgac ttgatgtgcc gtgagtgagc catagcggta
      301 cgtctatcct tggtccgtaa agatactgac gctagtttat aacggttgtg agaaggaagc
      361 gaagcggtca ggcgactagt ggtcttcatc ctaagacgga tgtaaaagac accattacgc
      421 tgataaaggt aggttcttga cttgagcatg tactgagacc ttaggtctgc tcactacttc
      481 aacaaagtta gtcacagtgt tggttacagt gagtaaaact atcggaaggc gttaagccgt
      541 atcccttagg attcagtaag ttagtagact ttaagaagtg tcatggagcg ggcga
//
LOCUS       DADMGC010000384          572 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.394, whole genome shotgun sequence.
ACCESSION   DADMGC010000384 DADMGC010000000
VERSION     DADMGC010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.394"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
BASE COUNT          136 a          112 c          164 g          160 t
ORIGIN      
        1 aaaaaactta tgcggggtgg atggtttcac gaagtgaggc catccacctg taagacaggg
       61 ttttgttttt atcccctgtt ttggtgatcg ggtgtgtgga aaaggttggg gtaagccgtt
      121 cgggggtgct tgttttgggg ggttaaaatt gtggttattt tttgcgcaat tctcgcgcgt
      181 gatccttgta tttatactta agggataaat ggcgggtatg aaatagtggt ttagcccagt
      241 aatgacgagg ctttgagtgg gttttgacag gtcaaagaaa atggagcaga attgaggcgt
      301 ttttaatcgg cgttggggag tgcgtcaaca ctccccaaca tttcgaatgt gtcacctcag
      361 cggcaaactc tggtgacatg tactggctcg caatgcacag gtacgtgatg aatataccac
      421 atcaaatcac agcctgccca gatcggagca ggcttaatgt cagaagataa attcctttcg
      481 gactacagcc cccgtgatgc agtttgggat acccagcgca cgcttaccga ttctgtcggg
      541 ggtatctacc agactgctgc tgaattcgag cg
//
LOCUS       DADMGC010000385          571 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.395, whole genome shotgun sequence.
ACCESSION   DADMGC010000385 DADMGC010000000
VERSION     DADMGC010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.395"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
BASE COUNT          138 a          112 c          161 g          160 t
ORIGIN      
        1 aaaaaactta tgcggggtgg atggtttcac gaagtgaggc catccacctg taagacaggg
       61 ttttgttttt attccctgtt ttggtgatcg ggtgcgtgga aaaggttggg gtaagccgtt
      121 cgggggtgct tgttttgggg gttaaaattg tggttatttt ttgcgcaatt ctcgcgcgtg
      181 atccttgtat ttatacttaa gggataaatg gcggatatga aatagtggtt tagcccagta
      241 atgacgaggc tttgagtggg ttttgacagg tcaaagaaaa tggagcagaa ttgaggcgtt
      301 tttaatcggc gttggggagt gcgtcaacac tccccaacat ttcgaatgtg tcacctcagc
      361 ggcaaactct ggtgacatgt actggctcac aatgcacagg tacgtgatga atataccaca
      421 tcaaatcaca gcctgcccag atcggagcag gcttaatgtc agaagataaa ttcctttcgg
      481 actacagccc ccgtgatgca gtttgggata cccagcgcac gcttaccgat tctgtcgggg
      541 gtatctacca gactgctgct gaattcgagc g
//
LOCUS       DADMGC010000386          566 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.396, whole genome shotgun sequence.
ACCESSION   DADMGC010000386 DADMGC010000000
VERSION     DADMGC010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.396"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..276
                     /locus_tag="JNZ73_004438"
     CDS             <1..276
                     /locus_tag="JNZ73_004438"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8734425.1"
                     /translation="KKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQLYNWRSK
                     QQNQQTSSERELEMSTEIARLKRQLAERDEELAILQKAATYFAKRLK"
     gene            273..>566
                     /locus_tag="JNZ73_004439"
     CDS             273..>566
                     /locus_tag="JNZ73_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8734426.1"
                     /translation="MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQ
                     FRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKA"
BASE COUNT          146 a          158 c          154 g          108 t
ORIGIN      
        1 aaaaaacccc gtaaacagca ttcgcctgaa tttcgcagtg aagccctgaa gcttgctgaa
       61 cgcatcggtg ttactgccgc agcccgtgaa ctcagcctgt atgaatcaca gctctacaac
      121 tggcgcagta aacagcaaaa tcagcagacg tcttctgaac gtgaactgga gatgtctacc
      181 gagattgcac gtctcaaacg ccagctggca gaacgggatg aagagctggc tatcctccaa
      241 aaggccgcga catacttcgc gaagcgcctg aaatgaagta tgtctttatt gaaaaacatc
      301 aggctgagtt cagcatcaaa gcaatgtgcc gcgtgctccg ggtggcccgc agcggctggt
      361 atacgtggtg tcagcggcgg acaaggataa gcacgcgtca gcagttccgc caacactgcg
      421 acagcgttgt cctcgcggct tttacccggt caaaacagcg ttacggtgcc ccacgcctga
      481 cggatgaact gcgtgctcag ggttacccct ttaacgtaaa aaccgtggcg gcaagcctgc
      541 gccgtcaggg actgagggca aaggcc
//
LOCUS       DADMGC010000387          566 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.397, whole genome shotgun sequence.
ACCESSION   DADMGC010000387 DADMGC010000000
VERSION     DADMGC010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.397"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..297
                     /locus_tag="JNZ73_004440"
     CDS             <1..297
                     /locus_tag="JNZ73_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HAY8734427.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="JNZ73_004441"
     CDS             complement(405..>566)
                     /locus_tag="JNZ73_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734428.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DADMGC010000388          562 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.398, whole genome shotgun sequence.
ACCESSION   DADMGC010000388 DADMGC010000000
VERSION     DADMGC010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.398"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..329)
                     /locus_tag="JNZ73_004442"
     CDS             complement(<1..329)
                     /locus_tag="JNZ73_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Member of the extracytoplasmic function sigma
                     factors which are active under specific conditions; binds
                     with the catalytic core of RNA polymerase to produce the
                     holoenzyme and directs bacterial core RNA polymerase to
                     specific promoter elements to initiate transcription; this
                     sigma factor regulates the genes for iron dicitrate
                     transport; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HAY8734429.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLET"
BASE COUNT          143 a          122 c          146 g          151 t
ORIGIN      
        1 gtctcgagtt ggctttcgcg ttcctcaggt gaaggcgctc ccccctccgg cataagcgcc
       61 agcatctcca gatacgcttt ttccagcgcg tttcggcgaa acaggtccac catcacgcgt
      121 ttggcgatag tgcagaggaa ggagcgagga tcgcggatcg tcgagagcgt ttcgctgacc
      181 attacccgca aaaaagtgtc ctgggcaatg tcatctgcat caaaagcaga ctggagtttg
      241 cgcgtcagcc agcttttcaa ccagccgtga tgtgtgccat aaagcgactc gaacgttaag
      301 gaagctgtgg tagtggcgcg gtcagacatg cggagtgcat caaaagttaa ttatcacgta
      361 gtcatattaa tatgagaatg gttatcatta caattggaaa taaaattgtt tccaatagac
      421 atttttaaca tgttgttttt ctaagtgtta taaggtaggt ataaaatggg atggagcctc
      481 tgcttctgac atgtgtcggt cagaatgact catgatgtgg tctgctatta ttgacatcct
      541 cactgcccta aaggatgggg at
//
LOCUS       DADMGC010000389          555 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.399, whole genome shotgun sequence.
ACCESSION   DADMGC010000389 DADMGC010000000
VERSION     DADMGC010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.399"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..>555
                     /locus_tag="JNZ73_004443"
     CDS             <1..>555
                     /locus_tag="JNZ73_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HAY8734430.1"
                     /translation="VNMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNEAFNRELAKLN
                     QSLRETLSKFNKTTSKNNYSENASNSLDVLATSAIKAGTAIYGFQTALEAYRKVMEIG
                     LKKEASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETFSSFAKFSAGAGDMNADQ
                     KESLFSNVIGTSRLMGLSTDEING"
BASE COUNT          187 a           87 c          121 g          160 t
ORIGIN      
        1 gagtgaatat gagcaagctt cgtgacttta atcctatgct tgagaagtat tttaaaagcc
       61 agtattacag cttaagcagt aaatccaaga cacttggaaa cgaagctttt aatcgtgagt
      121 tagcaaaatt aaatcaatct cttcgtgaaa cactttctaa gtttaataag accaccagca
      181 aaaataatta cagtgaaaat gcttcaaata gtctggatgt attagctaca agtgcaatca
      241 aagcaggtac agccatttat ggttttcaga cagccttaga agcttatagg aaggttatgg
      301 agattggtct gaaaaaagaa gcatcacaac gagcggcaca attcgtttta ggtgatgaag
      361 gtgctaaacg agctacagaa tttgttaaga atcttgctga tagtagtggt gtagaccaga
      421 ttgaaacatt tagcagtttt gctaagtttt ccgctggtgc tggtgatatg aatgcagacc
      481 agaaagaatc cttattttct aatgtgattg gtacatcccg actaatgggt ttaagcactg
      541 atgagattaa cggga
//
LOCUS       DADMGC010000390          534 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.400, whole genome shotgun sequence.
ACCESSION   DADMGC010000390 DADMGC010000000
VERSION     DADMGC010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.400"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            30..534
                     /locus_tag="JNZ73_004444"
                     /pseudo
     CDS             30..534
                     /locus_tag="JNZ73_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          136 a          134 c          154 g          110 t
ORIGIN      
        1 ctaaacttcc tgagaaagag gtaaacagga tgactaaaaa tactcgtttt tcccccgaag
       61 tccgtcaacg ggcagtccgt atggttctgg aaagtcaggg cgaatatgac tcacaatggg
      121 cgacaatttg ttccattgct ccaaagattg gctgtacgcc ggagactctg cgtgtctggg
      181 ttcgccagca tgagcgggat accgggggcg gtgatggagg gctcaccacc gctgaacgtc
      241 agcgtctgaa agagctggag cgtgaaaatc gtgaactgcg ccgcagtaac gatatccttc
      301 gccaggcttc cgcttatttt gcgaaggcgg agttcgaccg cctctggaaa aaatgatgcc
      361 actgctggat aagctgcgtg agcagtacgg ggtcggaccg ctatgcagcg aactgcatat
      421 tgccccgtca acgtattacc actgtcagca acagcgacat catccggata aacgcagtgc
      481 ccgtgcgcag cgcgatgact ggctgaagaa agagatacag cgcgtatacg atga
//
LOCUS       DADMGC010000391          533 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.401, whole genome shotgun sequence.
ACCESSION   DADMGC010000391 DADMGC010000000
VERSION     DADMGC010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.401"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            5..>533
                     /gene="fimC"
                     /locus_tag="JNZ73_004445"
     CDS             5..>533
                     /gene="fimC"
                     /locus_tag="JNZ73_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAY8734431.1"
                     /translation="MTFCLLAGILMFMAMMVAGRAEAGVALGATRVIYPAGQKQVQLA
                     VTNNDENSTYLIQSWEENADGVKDGRFIVTPPLFAMKGKKENTLRILDATNNQLPQDR
                     ESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPPDQAAEKLRFRRSA
                     NSLTLINPTPYYLTVT"
BASE COUNT          162 a          113 c          126 g          132 t
ORIGIN      
        1 ggaaataaca ttctgcttgc tggcaggtat cctgatgttc atggcaatga tggttgccgg
       61 acgcgctgaa gcaggagtgg ccttaggtgc gactcgcgta atttatccgg cagggcaaaa
      121 acaagtgcaa cttgccgtga caaataatga tgaaaatagt acctatttaa ttcaatcatg
      181 ggaggaaaat gccgatggtg taaaggatgg tcgttttatc gtgacgcctc ctctgtttgc
      241 gatgaaggga aaaaaagaga ataccttacg tattcttgat gcaacaaata accaattgcc
      301 acaggaccgg gaaagtttat tctggatgaa cgttaaagcg attccgtcaa tggataaatc
      361 aaaattgact gagaatacgc tacagctcgc aattatcagc cgcattaaac tgtactatcg
      421 cccggctaaa ttagcgttgc cacccgatca ggccgcagaa aaattaagat ttcgtcgtag
      481 cgcgaattct ctgacgctga ttaacccgac accctattac ctgacggtaa cag
//
LOCUS       DADMGC010000392          513 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.402, whole genome shotgun sequence.
ACCESSION   DADMGC010000392 DADMGC010000000
VERSION     DADMGC010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.402"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..502
                     /locus_tag="JNZ73_004446"
     CDS             <1..502
                     /locus_tag="JNZ73_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY8734432.1"
                     /translation="PTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQS
                     LYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGENALRSVAVG
                     RKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLPW
                     NFSSVK"
BASE COUNT          149 a          109 c          153 g          102 t
ORIGIN      
        1 tccgacagaa atgactcagg aagcgctcag acggatagca gagttatacg acatagaagc
       61 ggagatacgt ggcagtccgg cagaggaacg gcttgcagtc agaaaagcca gaagcgtcca
      121 gttgatgcag tcgttgtacg actggataca gttgcagagg aaaacgctgt cgaaacatgc
      181 ggagatggcg aaggcgttcg actatatcct gaatcactgg aatgcgctga acgagttctg
      241 tcgtgacggc tgggtggaaa tagacaacaa catcggtgaa aacgcgttac gatcggtggc
      301 ggttggaaga aaaaattatc tctttttcgg ctcagacaag ggaggagaaa gtgcggcgat
      361 catctacagt ctgctggtca cctgcaaaca gaacgaagtg gagccggagg actggttgcg
      421 cgaagtgatc gagaagctca atgactggcc gtcgaaccaa gtgcatgaac tgctgccctg
      481 gaacttctcg tctgtaaaat aatccttacg cta
//
LOCUS       DADMGC010000393          478 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.403, whole genome shotgun sequence.
ACCESSION   DADMGC010000393 DADMGC010000000
VERSION     DADMGC010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.403"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..>478
                     /locus_tag="JNZ73_004447"
     CDS             <1..>478
                     /locus_tag="JNZ73_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8734433.1"
                     /translation="RGLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKK
                     LAAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEV
                     ALEVEQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTR"
BASE COUNT           90 a          164 c          129 g           95 t
ORIGIN      
        1 cgcggccttc tgacccagat acatccggca ctggagcgcg ttctcggtcc gagacttgag
       61 cacccggcgg tactcgatct tctccagcga tatccctcac cagaaaaact cgcttcgctg
      121 ggtgagaaga agctggcagc ccagctctgc aaacttgcgc ctcgtctggg taaacgcctt
      181 gcagcagaca tagctcaggc actggccgaa caaaccgtcg tcgttcccgg cacgaatgcc
      241 gctgccgtag tactgccacg tctggcactc cagctcatca cgctgcgtaa gcaaagagac
      301 gaggtggcgc ttgaggtaga acagcgagtt cttgctcacc ctctttaccc ggtcctgacc
      361 agtatgcccg gagtcggtgt caggaccgca gccagactcc tcaccgaggt cgcctgccgc
      421 gccttcgcct ctgccgcaca tctcgctgct tatgctggcc ttgcgccggt aactcggc
//
LOCUS       DADMGC010000394          473 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.404, whole genome shotgun sequence.
ACCESSION   DADMGC010000394 DADMGC010000000
VERSION     DADMGC010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.404"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(88..237)
                     /gene="hokB"
                     /locus_tag="JNZ73_004448"
     CDS             complement(88..237)
                     /gene="hokB"
                     /locus_tag="JNZ73_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HAY8734434.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(337..>473)
                     /locus_tag="JNZ73_004449"
     CDS             complement(337..>473)
                     /locus_tag="JNZ73_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8734435.1"
                     /translation="FSNISELKDAVTEYIEYYNSRRISLKLKGLTPNEYRNQTYMPRV
                     "
BASE COUNT          120 a          110 c          115 g          128 t
ORIGIN      
        1 aacatgcact aactgaagtg taaaaggggt gccatgagaa aatgaatctg ctagtcagat
       61 gcgcggggaa aatccccgcg cttgccctta cctggacgtg caggccatga gcgcagcaac
      121 ctccttatca ccgtcccgga accgcagttc gtagagcgtt tgtcgggtca ggagtgtgaa
      181 tgccagaatc gtaatgcaga taatgagcag acacaccacc agagggttgc gcttcatagc
      241 ctttctcctt gccggatggc gggtaagagg ctaacatctg aattgcttgg ttcattcgtt
      301 ggcctcggtt tgtactgacc ccaaaaagtt ggacagttaa acacgaggca tataggtctg
      361 attccgatat tcatttggag tcagaccttt taatttcagg ctaattcttc tgctgttgta
      421 gtattcaata tattccgtaa cagcatcctt cagttcgctt atattactga act
//
LOCUS       DADMGC010000395          473 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.405, whole genome shotgun sequence.
ACCESSION   DADMGC010000395 DADMGC010000000
VERSION     DADMGC010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.405"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..410)
                     /locus_tag="JNZ73_004450"
     CDS             complement(<1..410)
                     /locus_tag="JNZ73_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HAY8734436.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEADVQTKQERNLTGVQTDVGVVFEH"
BASE COUNT          110 a          117 c           94 g          152 t
ORIGIN      
        1 tcatgttcga acaccactcc aacatctgtt tgaacacctg ttaaatttct ctcttgtttt
       61 gtttgaacat ctgcttcctt tctgcttctt ctggcctgaa cagatgcttt tcctgcggct
      121 gattttttgg ttaatttttc tctgacagat gccagatctt cctcaatccg aagatgcatc
      181 cattcgtcgc cgttatcaca aaaaaactcc cgcaaggatg gttcaacatc aacccatcgc
      241 tcgttagtca gacgggcaat ttttgccaac ctgtttttgg gtattggctt tcctgtttgc
      301 cagtaattga acatcagcaa caaatacgcg ccgtgctcct ctgctgacaa atgcatggtg
      361 tcagccagat aatcagctat gtacagttgc atgtatggta atgcggccat aattgccccg
      421 tatgatgcta cccggttgct tagaataagt gaggtagcct gagtttaacg gac
//
LOCUS       DADMGC010000396          471 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.406, whole genome shotgun sequence.
ACCESSION   DADMGC010000396 DADMGC010000000
VERSION     DADMGC010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.406"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
BASE COUNT          162 a           99 c          104 g          106 t
ORIGIN      
        1 gcaaataacg gatggcgtag ttttacactg agaaatgaaa gggatttgaa aagaaaaaaa
       61 cgcaaaagcg ggcgaaacga tatatacagt aaggaaagca ctctatccaa caaaccaccc
      121 acagttaatc ggaataaaag cagagtgctt atgaatgatc gcacgcccga aggttagtat
      181 ttctgcacag caattttgca aaaaaaggcg atcattcata acttaaacgt ctttcagtca
      241 ctccgggatt tcccatcatc gcagactgaa agactctgac tggagcgggc agcgggagcc
      301 tacgacaaat cgaagacaac cagctaacct attgtttctt aaagacaggt gaatccagtt
      361 ggttgtagtg atagtcacac atttagagat agaaggtcaa gacttgaagc acgtaatatt
      421 ccctctaaag gaatagaact cacggtctga ctacccggcc cttttggtct g
//
LOCUS       DADMGC010000397          470 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.407, whole genome shotgun sequence.
ACCESSION   DADMGC010000397 DADMGC010000000
VERSION     DADMGC010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.407"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..170
                     /locus_tag="JNZ73_004451"
     CDS             <1..170
                     /locus_tag="JNZ73_004451"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8734437.1"
                     /translation="QLYAWRSKLQQQMTSSERESELAAENARLKRQLAEQAEELAILQ
                     KAATYFAKRLK"
     gene            167..>470
                     /locus_tag="JNZ73_004452"
     CDS             167..>470
                     /locus_tag="JNZ73_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8734438.1"
                     /translation="MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTGISPRQQ
                     FRQHCDSVVLAAAFTRSKQRYGAPRLTDELRAQGYHFNVKTVAASLRRQGLRAKASR"
BASE COUNT          110 a          132 c          142 g           86 t
ORIGIN      
        1 ctcagctcta tgcgtggcgc agcaaactgc agcaacagat gacctcgtct gagcgtgaaa
       61 gtgaactggc cgctgaaaat gcccgcctga agcggcaact ggcggagcag gcagaggaac
      121 tggctatcct ccaaaaggcc gcgacatact ttgcgaagcg cctgaaatga agtatgtctt
      181 tattgaaaaa catcaggctg agttcagcat caaggcaatg tgccgcgtgc tccgggtggc
      241 ccgcagcggc tggtatacgt ggtgtcagcg gcggacagga ataagccctc gtcagcagtt
      301 ccgtcagcac tgcgacagcg ttgtcctcgc tgcggccttt acccggtcaa aacagcgtta
      361 cggtgcacca cgcctgacgg atgaactgcg tgctcagggt taccacttta acgtaaaaac
      421 cgtggcggca agcctgcgcc gtcaggggct gagggcaaag gcctcccgga
//
LOCUS       DADMGC010000398          464 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.408, whole genome shotgun sequence.
ACCESSION   DADMGC010000398 DADMGC010000000
VERSION     DADMGC010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.408"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..>464)
                     /locus_tag="JNZ73_004453"
     CDS             complement(<1..>464)
                     /locus_tag="JNZ73_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8734439.1"
                     /translation="TLSLKAAIKVKRYRSYRGEVGQTAPNVLQRDFKATRPNEKWVTD
                     VTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFKKLNPHEHPVLH
                     SDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLD"
BASE COUNT          124 a          107 c           93 g          140 t
ORIGIN      
        1 catcaagata aaaacactcc gactttaagg ttccaaagaa acactccacc acagcattat
       61 ccagacaatt gccttttctg gacatgcttt gtttaatacc atgttcttta aggatatttt
      121 gatatcttct catacgatac tgccatccct ggtcagagtg cagaacagga tgctcgtgag
      181 gattaagctt tttgaatgcc tgatcgagca tattctcaac catgttcatc actggtcttt
      241 ccgaaaggct gtaagaaata acttcgttgt tgaagagatc tattactgga gacaaataca
      301 gcttgcgccc attgactgca aattcagtaa catcggtaac ccacttctcg tttggccgcg
      361 tagccttgaa atctctttgg agaacattag gggcggtttg ccctacctct cctctgtaag
      421 agcggtatcg cttgacctta atcgctgctt taagtgagag ggtt
//
LOCUS       DADMGC010000399          464 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.409, whole genome shotgun sequence.
ACCESSION   DADMGC010000399 DADMGC010000000
VERSION     DADMGC010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.409"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..>464
                     /locus_tag="JNZ73_004454"
     CDS             <1..>464
                     /locus_tag="JNZ73_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY8734440.1"
                     /translation="QHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQ
                     RWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKL
                     HVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHY"
BASE COUNT          113 a          111 c          133 g          107 t
ORIGIN      
        1 ctcaacacgg ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
       61 aaccgggcag tgatgtgatt gtctgcgctg aaatggacga acattggggc tacgtcggtg
      121 ctaaatcacg tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg
      181 cgcacgtctt cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg
      241 cctttgaggt cgtggtatgg atgacggatg gctggccgct gtatgaatca cgcctgaagg
      301 gaaagctgca cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc
      361 tgagacaaca tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc
      421 tgcatgacaa ggtcatcggg cattatctga acataaaaca ctat
//
LOCUS       DADMGC010000400          462 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.410, whole genome shotgun sequence.
ACCESSION   DADMGC010000400 DADMGC010000000
VERSION     DADMGC010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.410"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..>462
                     /locus_tag="JNZ73_004455"
     CDS             <1..>462
                     /locus_tag="JNZ73_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HAY8734441.1"
                     /translation="YLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVG
                     WAMSFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMS
                     RRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRP"
BASE COUNT          111 a          113 c          145 g           93 t
ORIGIN      
        1 actaccttga aaggcagttc gccgtgaccg agccaaatca ggtgtggtgc ggtgatgtga
       61 cctatatctg gacgggtaag cgctgggcgt acctcgccgt tgttctcgac ctgttcgcaa
      121 gaaaaccagt gggctgggcc atgtcgttct cgccggacag caggctcacc atgaaagcgc
      181 tggaaatggc atgggaaacc cgtggtaagc ccggcggggt gatgttccac agcgatcagg
      241 gcagtcatta tacgagcagg cagttccggc agttattgtg gcgataccag atcagacaga
      301 gtatgagccg gcgcggaaac tgctgggata acagcccaat ggaacgcttc ttcaggagtc
      361 tgaagaacga atggatgccg gtggtgggtt acgtaagctt cagcgaggca gctcacgcca
      421 taacggacta tatcgttgga tattacagcg cactaagacc gc
//
LOCUS       DADMGC010000401          462 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.411, whole genome shotgun sequence.
ACCESSION   DADMGC010000401 DADMGC010000000
VERSION     DADMGC010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.411"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..183
                     /locus_tag="JNZ73_004456"
     CDS             <1..183
                     /locus_tag="JNZ73_004456"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734442.1"
                     /translation="QLEKTGITKDAIRKDMVPALRELMQAKHRAQKAVDELVDCVAEL
                     ETRVGKFEKLVQEALR"
     gene            278..>462
                     /locus_tag="JNZ73_004457"
     CDS             278..>462
                     /locus_tag="JNZ73_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HAY8734443.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRG"
BASE COUNT          137 a           91 c          136 g           98 t
ORIGIN      
        1 caactcgaaa aaacgggcat aacaaaagat gctatccgta aagatatggt acccgcgctt
       61 cgggaactga tgcaggcgaa gcatcgcgca cagaaagcag tagatgagct ggtggactgt
      121 gtggcagagc tggaaaccag agtcggaaag tttgaaaaac tggtgcagga ggcgctgcgc
      181 tgatgcgcca tgagtttgtt ttgccctggc ttgtgcgcga tcattatgtc cctggtggaa
      241 agcccgggat aaaaatcacc gaaccggaga gtatttgatg agcgtcaaaa ttcaaacgat
      301 accggaactg cttatccaga cacgaggtaa tatgaccgaa gtttcacgaa tgctgaactg
      361 taatcgcgcc acggtaagaa aatatgctga agataaagag ggcaaagggc acgccattgt
      421 tgatggtgtt ctgattgttc atcgtggatg ggacaggggt aa
//
LOCUS       DADMGC010000402          446 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.412, whole genome shotgun sequence.
ACCESSION   DADMGC010000402 DADMGC010000000
VERSION     DADMGC010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.412"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
BASE COUNT           89 a           97 c          100 g          160 t
ORIGIN      
        1 cagtcagacc ttgctgaaaa cataggaacg cgttaaaacg catacgggcg cgattctggt
       61 gtgtcttttg ttgctgtgtt ttgcctctat tgccagatcg cgctcattcc cctgaacttc
      121 tgttttcccg ctcaggtccc cgtcttcttc gcttccgaaa gcatgaaaaa aatccgtttc
      181 agctgtctct ggctggcaat tcctgcgtga ttcacatggc tgcatagcta tgcatgtggt
      241 taaaatttac cgggtgcgat cgcggcagtt tttcgggtgg tttgttgcca tttttacctg
      301 tctgctgccg tgatcgcgcc gaacgcgttt cagcggtgcg tacaatgaga tgttatggta
      361 aattcaagtc ttagatttta tgatgtttaa ttagcttttt ttgcgtttat aatgttcatc
      421 ttttttatta aaattgattc aatctt
//
LOCUS       DADMGC010000403          442 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.413, whole genome shotgun sequence.
ACCESSION   DADMGC010000403 DADMGC010000000
VERSION     DADMGC010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.413"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            211..>442
                     /locus_tag="JNZ73_004458"
     CDS             211..>442
                     /locus_tag="JNZ73_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY8734444.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          139 a          106 c           63 g          134 t
ORIGIN      
        1 tcgaacctcg cttaccaacg cgttgtcagt catctccccc ctccctggtg catcgttata
       61 tccgtgatga gcgatttttg atattaatat ctttcaccat ccgctatttt caccacttca
      121 gaaaacaaac ttatttttat taaaatcaat tctttttctt tgaacattca actgcgatat
      181 ttgtaaaaac attaaacagg caacaataaa atgacattac caacaactat ttattcattc
      241 cccgcatatt taccccgttt ttcgtccacc gacaaaccag caaaattaaa atttcatcaa
      301 tacgcacgcg ctacattact gtcaaatcgt ggaagagatc ataattgcga tggcagacgt
      361 actgttgaga tccacaaact agatctctct gactggcaag cattcaataa gttagcaacg
      421 aagtgtaatg cttacgatgg ag
//
LOCUS       DADMGC010000404          439 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.414, whole genome shotgun sequence.
ACCESSION   DADMGC010000404 DADMGC010000000
VERSION     DADMGC010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.414"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            26..439
                     /locus_tag="JNZ73_004459"
                     /pseudo
     CDS             26..439
                     /locus_tag="JNZ73_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          124 a          100 c          119 g           96 t
ORIGIN      
        1 cagacaacca gtatcactta tttaagtgat agtcttaata ctagttttta gactagtcat
       61 tggagaacag atgattgatg tcttagggcc ggagaaacgc agacggcgta ccacacagga
      121 aaagatcgca attgttcagc agagctttga accggggatg acggtctccc tcgttgcccg
      181 gcaacatggt gtagcagcca gccagttatt tctctggcgt aagcaatacc aggaaggaag
      241 tcttactgct gtggccgccg gagaacaggt tgttcctgcc tctgaacttg ctgccgccat
      301 gaagcagatt aaagaactcc agcgcctgct cggcaagaaa acgatggaaa atgaactcct
      361 caaagaagcc gttgaatatg gacgggcaaa aaagtggata gcgcacgcgc ccttattgcc
      421 cggggatggg gagtaagct
//
LOCUS       DADMGC010000405          438 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.415, whole genome shotgun sequence.
ACCESSION   DADMGC010000405 DADMGC010000000
VERSION     DADMGC010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.415"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..>438
                     /locus_tag="JNZ73_004460"
     CDS             <1..>438
                     /locus_tag="JNZ73_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY8734445.1"
                     /translation="PLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKT
                     AQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQ
                     MELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYY"
BASE COUNT          100 a          140 c          116 g           82 t
ORIGIN      
        1 cctcttcacc agattgtgaa tcgcctggac atcatgctgc cgggcaatcg ccccttcgtt
       61 gcccccagtg ccgttattca ggcccgccag cgcctgggaa gtgaggctgt ccgccgcgtg
      121 ttcacgaaaa cagcgcagct ctggcataac gccacgccgc atccgcactg gtgcggcctg
      181 accctgctgg ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca
      241 gccttccccc gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc
      301 tgccagatgg aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac
      361 agcgaaaatg agcttgctga gcaacttata gaacaaaccg gcgataacac tctgacgtta
      421 atggataaag gttattac
//
LOCUS       DADMGC010000406          428 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.417, whole genome shotgun sequence.
ACCESSION   DADMGC010000406 DADMGC010000000
VERSION     DADMGC010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.417"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            108..>428
                     /locus_tag="JNZ73_004461"
     CDS             108..>428
                     /locus_tag="JNZ73_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734446.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPCGVNIRGVGKASQLRAKSGLTGS
                     VLRLS"
BASE COUNT          121 a           87 c          105 g          115 t
ORIGIN      
        1 attttccgaa tttgctgaag tatgaccctg atcagttcgg tccggattta attgagcaac
       61 tagctcaatc cggtaagtat tcgcaggata acaccaaagg cgatgccatg attggcgtca
      121 agcagccttt accaaaagca gttttaagaa ctcagcatga caaaaataaa gaagcaataa
      181 gtatcctgga ttttggtgtt attgatgatg gtgtgacaga taattaccag gcaatacaaa
      241 atgcaataga tgccgttgct tcactaccct ccggcgggga gctgtttatc cctgcgagca
      301 accaagcggt ggggtatatt gttggatcca ctttgcttat tccttgcggt gttaacatca
      361 gaggggttgg taaggcatcg caactccgag caaaaagcgg acttacagga tctgtgttaa
      421 ggctgtct
//
LOCUS       DADMGC010000407          426 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.418, whole genome shotgun sequence.
ACCESSION   DADMGC010000407 DADMGC010000000
VERSION     DADMGC010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.418"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..111)
                     /locus_tag="JNZ73_004462"
     CDS             complement(<1..111)
                     /locus_tag="JNZ73_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAY8734447.1"
                     /translation="MPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGW"
BASE COUNT          130 a          110 c          114 g           72 t
ORIGIN      
        1 ccaaccggtt aaggggcgct gttgaataag acttatggca cctttcagaa tacgtatacg
       61 ctggtcattc gatgatgaat gcgtaaagtc agccgttgtc tcccctggca tggcatagtt
      121 tgagataccg acacaccagc acagcaagga ggcgacgaag atcaagcgca acagaccgct
      181 aaggggacgt ccgcgccagc ggggtctgtc cgcgagagcg gccagcacga tgacgcaaaa
      241 taaaccaaga aggccgacac ggctttgtgt aaggactaaa gctgtagaca gagggtataa
      301 cgacaacaaa catatcaacg tcagagaacg agtacgcgcc gttaaagcca gatacaacgc
      361 tacgccgcaa cctgttgcca gccagctcgc aagaacattt cgctgctgga aaatgccata
      421 cggaaa
//
LOCUS       DADMGC010000408          408 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.419, whole genome shotgun sequence.
ACCESSION   DADMGC010000408 DADMGC010000000
VERSION     DADMGC010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.419"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(7..225)
                     /locus_tag="JNZ73_004463"
     CDS             complement(7..225)
                     /locus_tag="JNZ73_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734448.1"
                     /translation="MKAVARANNHPYKSVFADFITGHPSCTVCFWETFHKMYPDSPYE
                     YVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT           90 a           99 c          108 g          111 t
ORIGIN      
        1 tgaaaatcac caccacttcg ggtcatccgc cttcatctcc gcttctgttt cgtataaatc
       61 aaaacgacgg caggtatggc agaacgtgac gtattcgtac ggtgaatccg ggtacatttt
      121 gtggaaggtt tcccagaaac agaccgtgca ggagggatga ccggtgataa agtctgcaaa
      181 tacggactta taagggtgat tattggctct ggcgacggct ttcagaaccc tctttcacca
      241 ttctggtgtg gactttctgg tgctccaggt tgtgtgacat gggaactcat tctggatggt
      301 tactctgaaa gctcatattc tgccacaccc cgatttgcag ccgccaggct gccgtggttc
      361 ttacgccaga agggatttgt aactggtctg gtcggctccg gcgtatca
//
LOCUS       DADMGC010000409          403 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.420, whole genome shotgun sequence.
ACCESSION   DADMGC010000409 DADMGC010000000
VERSION     DADMGC010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.420"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
BASE COUNT          118 a           87 c          106 g           92 t
ORIGIN      
        1 taaatcggaa ccccaaaggg gacccccacg atttagcatt tgacgagatt aactctcgga
       61 gatggcatca tgcttttaac ggactttaaa aaacggtgaa cggatgcgga ttttaatccg
      121 tgtctgtgag gcgttgagca acgagcaaag cgaggcggta gaaagagagc cagcggcagg
      181 ctcaggacga taatccgggg aaaatgtacc actcgcagag tgttaacatt tgacatcgga
      241 ttgtctatcc tgcattggtg tcgagttggc aaggctgagg ataatgctga ggataatgca
      301 ggatagacag agcactagag attgcatgca atctcgtacc tatcacctca gcctgccgct
      361 ggctcttttc cagaactaag aatgcgttct taaaaatcca aac
//
LOCUS       DADMGC010000410          396 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.421, whole genome shotgun sequence.
ACCESSION   DADMGC010000410 DADMGC010000000
VERSION     DADMGC010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.421"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..>396
                     /locus_tag="JNZ73_004464"
     CDS             <1..>396
                     /locus_tag="JNZ73_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8734449.1"
                     /translation="NRKKELTEPQIREGSWRKERRTSYSREFKVRLAKQALQPGAVVA
                     RIAREHDINNNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNK
                     HDERPEGAPGNVPRCELHLKSGVVKLFDP"
BASE COUNT          114 a           98 c          108 g           76 t
ORIGIN      
        1 ccaacagaaa gaaggaatta acagagcctc aaatcagaga aggctcatgg cgaaaagaac
       61 ggcgaaccag ctatagccgt gaatttaagg tccgtctggc taagcaggcg ttacagcctg
      121 gggctgttgt tgcccggatc gccagagaac acgatatcaa taataacctg ctgtttaaat
      181 ggaaaagcca gtacgaggac ggcttactga gcgatgatga catacaggaa tgcatgcctg
      241 tcccggtggc actgactgat acgccggagc cgaccagacc agttacaaat cccttctggc
      301 gtaacaagca tgatgagcgc cctgaggggg ctcccggaaa cgtcccacgg tgcgagctgc
      361 atcttaaatc aggtgtggta aaactgtttg accctc
//
LOCUS       DADMGC010000411          362 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.422, whole genome shotgun sequence.
ACCESSION   DADMGC010000411 DADMGC010000000
VERSION     DADMGC010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.422"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..>362
                     /locus_tag="JNZ73_004465"
     CDS             <1..>362
                     /locus_tag="JNZ73_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY8734450.1"
                     /translation="LRKAVAVAIGGGAIAIASVLITGPGGNDGLEGVSYIPYKDIVGV
                     WTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGALYSFV
                     YNVGAGNFRTSMLLRKIN"
BASE COUNT          101 a           90 c           87 g           84 t
ORIGIN      
        1 cattacgcaa ggctgttgca gttgctattg gtggtggggc tattgccata gcgtctgtgc
       61 tcatcactgg cccaggtggt aacgatggtc tggaaggtgt cagctacata ccatacaaag
      121 atatcgttgg cgtatggact gtatgccacg gacacaccgg aaaagacatc atgctcggta
      181 aaacgtatac cgaagcagaa tgcaaagccc tcctgaataa agaccttgcc actgtcgcca
      241 gacaaattaa cccgtacatc aaagtcgata taccggaaac aacgcgcggc gctctttact
      301 cgttcgttta caacgtgggc gctggcaatt tcagaacatc gatgcttctt cgcaaaataa
      361 ac
//
LOCUS       DADMGC010000412          351 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.423, whole genome shotgun sequence.
ACCESSION   DADMGC010000412 DADMGC010000000
VERSION     DADMGC010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.423"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..208)
                     /locus_tag="JNZ73_004466"
     CDS             complement(<1..208)
                     /locus_tag="JNZ73_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY8734451.1"
                     /translation="MSKIDYQALRAKAEKATKGSYIVGHTSVNRHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDVEVVNK"
     gene            complement(205..>351)
                     /locus_tag="JNZ73_004467"
     CDS             complement(205..>351)
                     /locus_tag="JNZ73_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HAY8734452.1"
                     /translation="PGILPAEMIIRQRVKPMPSREELLKRKSFGSVNDNKYLNAMWRK
                     GGNQ"
BASE COUNT           80 a           94 c           64 g          113 t
ORIGIN      
        1 ttttgttgac cacttcaaca tctgtttcaa ccaggcagtt aacatgacat tccgcaatca
       61 caccaccggg ttctcctttc catttttgac aaacaaaaac tcctgttaaa ttgccgtgcc
      121 gattaacaga tgtatgccct acgatgtagc ttcctttagt tgctttttct gccttggcac
      181 gcagtgcctg atagtcaatc ttgctcactg gttgcctcct ttgcgccaca tcgcattcag
      241 atatttgttg tcattaacag aaccgaaact ctttctctta agcaattcct ctctcgatgg
      301 cattggcttt acgcgctggc gaataatcat ttctgccgga agaatgccgg g
//
LOCUS       DADMGC010000413          346 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.425, whole genome shotgun sequence.
ACCESSION   DADMGC010000413 DADMGC010000000
VERSION     DADMGC010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.425"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            24..100
                     /locus_tag="JNZ73_004468"
     tRNA            24..100
                     /locus_tag="JNZ73_004468"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="JNZ73_004469"
     tRNA            143..218
                     /locus_tag="JNZ73_004469"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           98 a           72 c           87 g           89 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaagtt ttgctcttta aaaatctgga
      301 tcaagctgaa aattgaaaca ctgaacaacg agagttgttc gtgagt
//
LOCUS       DADMGC010000414          340 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.426, whole genome shotgun sequence.
ACCESSION   DADMGC010000414 DADMGC010000000
VERSION     DADMGC010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.426"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            229..336
                     /gene="ldrD"
                     /locus_tag="JNZ73_004470"
     CDS             229..336
                     /gene="ldrD"
                     /locus_tag="JNZ73_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HAY8734453.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           90 a           79 c           99 g           72 t
ORIGIN      
        1 gagacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aaggattccg
       61 gtgaggcgca atgttgcggg ggcttaatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acaccacggg ggccattctt gcatccaaac
      181 aatacaagaa tagccgcgaa acgttgtcat tacaatacag gcggctatat gacgttcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg tattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg
//
LOCUS       DADMGC010000415          338 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.427, whole genome shotgun sequence.
ACCESSION   DADMGC010000415 DADMGC010000000
VERSION     DADMGC010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.427"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            95..250
                     /locus_tag="JNZ73_004471"
                     /pseudo
     CDS             95..250
                     /locus_tag="JNZ73_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT           99 a           80 c           87 g           72 t
ORIGIN      
        1 gagcgtgcgg caacaacagg acgaacaggg gcggttttca atttgcagcc gtcaggctgc
       61 cgtggttcac tccaacaacg gagacaggta aattatggac gaaaagaagc ttaaagccct
      121 tgcggctgaa ctggctaaag gcctcaaaac cgaggccgac ctcaatcagt tttcccgtat
      181 gcagacgaaa ttaactgtcg aaacggtgct taatgcggaa ctaactgacc atctcgggca
      241 tgaaaaaagc tacatccggt agtaaacatt atcagtgggt tatgcgatat gatttgagcc
      301 gttttcagac aatcgcaggg ccagtacctc gtcagtca
//
LOCUS       DADMGC010000416          331 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.428, whole genome shotgun sequence.
ACCESSION   DADMGC010000416 DADMGC010000000
VERSION     DADMGC010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.428"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..>331
                     /locus_tag="JNZ73_004472"
     rRNA            <1..>331
                     /locus_tag="JNZ73_004472"
                     /product="16S ribosomal RNA"
BASE COUNT           85 a           75 c          111 g           60 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga c
//
LOCUS       DADMGC010000417          329 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.430, whole genome shotgun sequence.
ACCESSION   DADMGC010000417 DADMGC010000000
VERSION     DADMGC010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.430"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..290
                     /locus_tag="JNZ73_004473"
     CDS             <1..290
                     /locus_tag="JNZ73_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8734454.1"
                     /translation="EDINKPISIAAANRFVNRIRGGMDLGYWRTHDFRRTLVTRLSEM
                     NVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELHADKLFWHIRSISD"
BASE COUNT          102 a           74 c           80 g           73 t
ORIGIN      
        1 gagaagacat aaacaaaccg atcagcatcg ctgcagccaa ccgattcgta aatagaataa
       61 ggggagggat ggatctaggt tactggcgaa cacatgattt cagaagaacg cttgttacac
      121 ggctgtccga gatgaatgtc gagcctcatg ttactgagcg aatgctcggt catgaacttg
      181 gcgggataat gtccgtatac aataaacacg actggataga ggctcagcgc aaagcgtatg
      241 agcttcacgc tgataaattg ttctggcaca tcaggagcat ttctgattaa cgccaccgtt
      301 aagaatccac ccttcaacag cttcacgaa
//
LOCUS       DADMGC010000418          325 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.431, whole genome shotgun sequence.
ACCESSION   DADMGC010000418 DADMGC010000000
VERSION     DADMGC010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.431"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(1..325)
                     /locus_tag="JNZ73_004474"
                     /pseudo
     CDS             complement(1..325)
                     /locus_tag="JNZ73_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT           94 a           79 c           53 g           99 t
ORIGIN      
        1 ggctgtacgc ttccccatgt gatttgcctc gccacttcac cgggctcagg accccgtagg
       61 atcttcaccg aaaaaaatgc atcaccagtt gctttattat ttgttttagg tagacctgtt
      121 actaatttat acctactctg accaagtctg gataagaaag gcacacctgc acatttctac
      181 ttggaaatgt tttcttttac cattttgctc aaaaatttca acatccaata ttcctcgtac
      241 agaactactc aagtcctgaa tagaaaaagt tccagctggt acagtagagt catatattac
      301 tcgaccttgt tgagatacaa caaca
//
LOCUS       DADMGC010000419          323 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.432, whole genome shotgun sequence.
ACCESSION   DADMGC010000419 DADMGC010000000
VERSION     DADMGC010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.432"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            134..209
                     /locus_tag="JNZ73_004475"
     tRNA            134..209
                     /locus_tag="JNZ73_004475"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:167..169,aa:Val,seq:tac)
     gene            212..287
                     /locus_tag="JNZ73_004476"
     tRNA            212..287
                     /locus_tag="JNZ73_004476"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:245..247,aa:Lys,seq:ttt)
BASE COUNT           78 a           75 c           96 g           74 t
ORIGIN      
        1 gctaaggtgg aagcggtagt aaaatgtgaa ggataacgtt gcatcagcaa cggcgccgaa
       61 gggcgagacg aagtcgagtc atcctgcacg acccaccact aacatagtta gttgtagtat
      121 ccagcgtagt gtcgggtgat tagctcagct gggagagcac ctcccttaca aggagggggt
      181 cggcggttcg atcccgtcat cacccaccac cgggtcgtta gctcagttgg tagagcagtt
      241 gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccacta attacggtgg
      301 gttcggtgga agtagtttgt agt
//
LOCUS       DADMGC010000420          322 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.433, whole genome shotgun sequence.
ACCESSION   DADMGC010000420 DADMGC010000000
VERSION     DADMGC010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.433"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..>322
                     /locus_tag="JNZ73_004477"
     CDS             <1..>322
                     /locus_tag="JNZ73_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734455.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLLGGLSCLTIPCFAWLAD
                     RFGRR"
BASE COUNT           72 a           74 c           83 g           93 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctgttag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gc
//
LOCUS       DADMGC010000421          309 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.434, whole genome shotgun sequence.
ACCESSION   DADMGC010000421 DADMGC010000000
VERSION     DADMGC010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.434"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            71..146
                     /locus_tag="JNZ73_004478"
     tRNA            71..146
                     /locus_tag="JNZ73_004478"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:105..107,aa:Glu,seq:ttc)
BASE COUNT           85 a           68 c           72 g           84 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgata gaaagtgaaa agcaaggcgt cttgcgaagc
       61 agactgatac gtcaccttcg tctagaggcc taggacaccg ccctttcacg gcggtaacag
      121 gggttcgaat cccctagggg acgccacttg ctggtttgtg agtgaaagtc acctgcctta
      181 atatctcaaa actcatcttc gggtgatgtt tgagatattt gctctttaaa aatctggatc
      241 aagctgaaaa ttgaaacact gaacaatgaa agttgttcgt gagtctctca aattttcgca
      301 actctgaag
//
LOCUS       DADMGC010000422          306 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.435, whole genome shotgun sequence.
ACCESSION   DADMGC010000422 DADMGC010000000
VERSION     DADMGC010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.435"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..>306
                     /locus_tag="JNZ73_004479"
     CDS             <1..>306
                     /locus_tag="JNZ73_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY8734456.1"
                     /translation="YREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKM
                     AEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRG"
BASE COUNT           93 a           52 c           95 g           66 t
ORIGIN      
        1 gatacaggga gataaaacag acgatgcaac tgagcaggct gacgctgaga agtaaaaagc
       61 cggagcttgt ggagcaagag ctgtggggtg tcttactggc ttataatctg gtgagatatc
      121 agatgattaa aatggcggaa catctgaaag gttactggcc gaatcaactg agtttctcag
      181 aatcatgcgg aatggtgatg agaatgctga tgacattgca gggcgcttca ccgggacgta
      241 taccggagct gatgcgcgat cttgcaagta tgggacaact tgtgaaatta ccgacgagaa
      301 ggggaa
//
LOCUS       DADMGC010000423          295 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.438, whole genome shotgun sequence.
ACCESSION   DADMGC010000423 DADMGC010000000
VERSION     DADMGC010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.438"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..>295)
                     /locus_tag="JNZ73_004480"
     CDS             complement(<1..>295)
                     /locus_tag="JNZ73_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY8734457.1"
                     /translation="MDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLRYNSHRTK
                     REEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITETVR"
BASE COUNT           70 a           93 c           55 g           77 t
ORIGIN      
        1 ttctcactgt ctccgttatg atgtacacca cttcttccag taaccgccgt ttcgccggac
       61 tcaaaaaacc gtagtacctc accatacgga accccttatc cgccacatgc caggagaacc
      121 tttccatgaa ctcatctcca ctcatcaaca ggtattcttc ccgttttgtt cggtgactgt
      181 tgtaacgcag accgatttca tcctgaccgg cataatgctc cagacgactc atcggcaccg
      241 gtggcttttt caggtaagag ccaaagtaca ccgccacatg ggtggcatta tccat
//
LOCUS       DADMGC010000424          292 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.439, whole genome shotgun sequence.
ACCESSION   DADMGC010000424 DADMGC010000000
VERSION     DADMGC010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.439"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            153..>292
                     /locus_tag="JNZ73_004481"
     CDS             153..>292
                     /locus_tag="JNZ73_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8734458.1"
                     /translation="MLHEEKLARHQRKQAMYTRMVAFPAVKMFEEYDFTFATGAPQKQ
                     LQS"
BASE COUNT           82 a           71 c           77 g           62 t
ORIGIN      
        1 cggtggtcgg tttacgctta ccgcaggaca ggtggtaact gaaaaatagt ttgctgaggc
       61 gactggaaac agaagggcaa gatcgattcc gacagcagtg atggtcagct acctcatcga
      121 taactttgca acagtgccct tcttgaagca tcatgcttca tgaagaaaaa ctggcacgtc
      181 atcaacgtaa acaggcgatg tatacccgaa tggtagcctt cccggcggtg aaaatgttcg
      241 aagagtatga cttcacgttc gccaccggag caccgcagaa gcagctccag tc
//
LOCUS       DADMGC010000425          289 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.denovo.440, whole genome shotgun sequence.
ACCESSION   DADMGC010000425 DADMGC010000000
VERSION     DADMGC010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.denovo.440"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..>289
                     /locus_tag="JNZ73_004482"
     CDS             <1..>289
                     /locus_tag="JNZ73_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8734459.1"
                     /translation="ILRRTDDWMDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWAL
                     LRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESN"
BASE COUNT           71 a           73 c           82 g           63 t
ORIGIN      
        1 cattctcaga cgaaccgatg actggatgga tggccgccgc agtcgtcaca ctgatgatac
       61 ggatgtgctt ctccgtatac accatgttat cggagagctg cccacgtatg gttatcgtcg
      121 ggtatgggcg ctgcttcgca gacaggcaga acttgatggt atgcctgcga tcaatgccaa
      181 acgtgtttac cggatcatgc gccagaatgc gctgttgctt gagcgaaaac ctgctgtacc
      241 gccatcgaaa cgggcacata caggcagagt ggccgtgaaa gaaagcaat
//
LOCUS       DADMGC010000426         4250 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.guided.220, whole genome shotgun sequence.
ACCESSION   DADMGC010000426 DADMGC010000000
VERSION     DADMGC010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.guided.220"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            70..285
                     /locus_tag="JNZ73_004483"
     CDS             70..285
                     /locus_tag="JNZ73_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY8734460.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            278..664
                     /locus_tag="JNZ73_004484"
     CDS             278..664
                     /locus_tag="JNZ73_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HAY8734461.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            672..1358
                     /locus_tag="JNZ73_004485"
     CDS             672..1358
                     /locus_tag="JNZ73_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY8734462.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1336..1959)
                     /gene="tetR(B)"
                     /locus_tag="JNZ73_004486"
     CDS             complement(1336..1959)
                     /gene="tetR(B)"
                     /locus_tag="JNZ73_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HAY8734463.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2041..3246
                     /gene="tet(B)"
                     /locus_tag="JNZ73_004487"
     CDS             2041..3246
                     /gene="tet(B)"
                     /locus_tag="JNZ73_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HAY8734464.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3359..3952)
                     /gene="tetC"
                     /locus_tag="JNZ73_004488"
     CDS             complement(3359..3952)
                     /gene="tetC"
                     /locus_tag="JNZ73_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HAY8734465.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4040..4216
                     /locus_tag="JNZ73_004489"
                     /pseudo
     CDS             4040..4216
                     /locus_tag="JNZ73_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1201 a          759 c          893 g         1397 t
ORIGIN      
        1 tctgaacata aaacactatc aataagttgg agtcattacc gaccactatt aagtcatgta
       61 gctggtttta tttcaattga acgttttcaa agtctagcta cagaaggaaa aatgttatcg
      121 ctatcttggt gggaaaacga atacgcagtt ctgcaatgga aaaatcatgt tttacatgcg
      181 aaagctcaac aagaagggcg agagtcaata tttgattttt acaaaattag tattgctcat
      241 attactcgcg aatattcatt taaaaaggac aaggataatg tttgatgttc acgttgtttt
      301 agataatcaa ataggacaat tagcattact aggaaaaaca ttaggtaata aaggtattgg
      361 attggaaggg ggagggatat ttacggttgg tgatgaatgc catgctcatt ttcttgttga
      421 acaaggaaag gaagctaaaa tagcgctaga gcaagctgga ctgttagtac ttgcgatccg
      481 gacaccatta attcgtaagt taaaacagga aaaaccgggg gaacttggcg aaatagcacg
      541 agtattggcg gagaataaca ttaatatttt agtgcaatac agtgaccatg ctaaccaact
      601 gatattaata acggacaatg atagtatggc tgcatctgtt acgctccctt gggcaataaa
      661 gtgaacttgc gatggctaat ttaatacgaa aagaggttac ctttgagtcc tcaatagccg
      721 cgataggggc ggctatgtct gacatttcac gagttaaaat actcagtgct ttgatggatg
      781 ggcgagcttg gacggccact gagctaagtt ctgtggcgaa tatatcagct tcaacggcga
      841 gcagtcattt atctaaatta ttagattgcc agctaatcac agtagtagct caaggcaagc
      901 atcgttattt tcggctagca ggaaaagata ttgctgaatt gatggaaagt atgatgggga
      961 tctccttaaa ccatggcgta catgccaaag tttccacgcc agtgcattta cgaaaagcac
     1021 gtacttgcta tgatcattta gctggcgaag ttgccgttaa gatctatgat tccctttgtc
     1081 aacagcaatg gatcactgaa aatggttcaa tgatcacatt aagtggtatt caatattttc
     1141 atgaaatggg aattgacgtt ccttccaaac attcacgtaa aatctgttgt gcgtgtttag
     1201 attggagtga acgccgtttc catttaggtg ggtacgttgg agccgcatta ttttcgcttt
     1261 atgaatctaa agggtggtta actcgacatc ttggttaccg tgaagttacc atcacggaaa
     1321 aaggttatgc tgcttttaag acccactttc acatttaagt tgtttttcta atccgcatat
     1381 gatcaattca aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat
     1441 agcttgtcgt aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc
     1501 gacttgatgc tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag
     1561 tgcatataat gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc
     1621 gagagtttca tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg
     1681 atgacttagt aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct
     1741 ttccccttct aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc
     1801 gtcgagcaaa gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag
     1861 cttctgggcg agtttacggg ttgttaaacc ttcgattccg acctcattaa gcagctctaa
     1921 tgcgctgtta atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt
     1981 gacactctat cattgataga gttattttac cactccctat cagtgataga gaaaagtgaa
     2041 atgaatagtt cgacaaagat cgcattggta attacgttac tcgatgccat ggggattggc
     2101 cttatcatgc cagtcttgcc aacgttatta cgtgaattta ttgcttcgga agatatcgct
     2161 aaccactttg gcgtattgct tgcactttat gcgttaatgc aggttatctt tgctccttgg
     2221 cttggaaaaa tgtctgaccg atttggtcgg cgcccagtgc tgttgttgtc attaataggc
     2281 gcatcgctgg attacttatt gctggctttt tcaagtgcgc tttggatgct gtatttaggc
     2341 cgtttgcttt cagggatcac aggagctact ggggctgtcg cggcatcggt cattgccgat
     2401 accacctcag cttctcaacg cgtgaagtgg ttcggttggt taggggcaag ttttgggctt
     2461 ggtttaatag cggggcctat tattggtggt tttgcaggag agatttcacc gcatagtccc
     2521 ttttttatcg ctgcgttgct aaatattgtc gctttccttg tggttatgtt ttggttccgt
     2581 gaaaccaaaa atacacgtga taatacagat accgaagtag gggttgagac gcaatcgaat
     2641 tcggtataca tcactttatt taaaacgatg cccattttgt tgattattta tttttcagcg
     2701 caattgatag gccaaattcc cgcaacggtg tgggtgctat ttaccgaaaa tcgttttgga
     2761 tggaatagca tgatggttgg cttttcatta gcgggtcttg gtcttttaca ctcagtattc
     2821 caagcctttg tggcaggaag aatagccact aaatggggcg aaaaaacggc agtactgctc
     2881 ggatttattg cagatagtag tgcatttgcc tttttagcgt ttatatctga aggttggtta
     2941 gttttccctg ttttaatttt attggctggt ggtgggatcg ctttacctgc attacaggga
     3001 gtgatgtcta tccaaacaaa gagtcatcag caaggtgctt tacagggatt attggtgagc
     3061 cttaccaatg caaccggtgt tattggccca ttactgtttg ctgttattta taatcattca
     3121 ctaccaattt gggatggctg gatttggatt attggtttag cgttttactg tattattatc
     3181 ctgctatcga tgaccttcat gttaacccct caagctcagg ggagtaaaca ggagacaagt
     3241 gcttagttat ttcgtcacca aatgatgtta ttccgcgaaa tataatgacc ctcttgataa
     3301 cccaagaggg cattttttac gataaagaag atttagcttc aaataaaacc tatctatttt
     3361 atttatcttt caagctcaat aaaaagccgc ggtaaatagc aataaattgg ccttttttat
     3421 cggcaagctc ttttaggttt ttcgcatgta ttgcgatatg cataaaccag ccattgagta
     3481 agtttttaag cacatcatca tcataagctt taagttggtt ctcttggatc aatttgctga
     3541 caatggcgtt taccttacca gtaatgtatt caaggctaat tttttcaagt tcattccaac
     3601 caatgatagg catcacttct tggataggga taaggttttt attattatca ataatataat
     3661 caagataatg ttcaaatata ctttctaagg cagaccaacc atttgttaaa tcagtttttg
     3721 ttgtgatgta ggcatcaatc ataattaatt gctgcttata acaggcactg agtaattgtt
     3781 ttttattttt aaagtgatga taaaaggcac ctttggtcac caacgctttt cccgagatct
     3841 catctattga aacagcttga tagccttttt caacaaacaa tattcgtgct gagttaacca
     3901 gtgattgata ggtactctta aaattttctt gttgatgatt tttattttcc atgatagatt
     3961 taaaataaca taccgtcagt atgtttatgg tatcatgatg atgtggtcgt gacaatctta
     4021 agaacattta ggttatttta tgtatattga acagcattct cgctatcaaa ataaagctaa
     4081 taacatccaa ttaagatatg atgataagca gtttcataca acggttatca aagatgttct
     4141 attatggatt gaacataatt tagatcagtc tttactgctt gatgatgtgg cgaataaagc
     4201 gggttatacc aagtgggtaa tgactccaac ttactgatag tgttttatgt
//
LOCUS       DADMGC010000427         3258 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.guided.238, whole genome shotgun sequence.
ACCESSION   DADMGC010000427 DADMGC010000000
VERSION     DADMGC010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.guided.238"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..473
                     /locus_tag="JNZ73_004490"
     CDS             <1..473
                     /locus_tag="JNZ73_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY8734466.1"
                     /translation="MKVEALANFPIERDLVVDMTHFIESLEAIKPYIIGNSRTADQGT
                     NIQTPAQMAKYHQFSGCINCGLCYAACPQFGLNPEFIGPAAITLAHRYNEDSRDHGKK
                     ERMAQLNSQNGVWSCTFVGYCSEVCPKHVDPAAAIQQGKVESSKDFLIATLKPR"
     gene            484..879
                     /gene="frdC"
                     /locus_tag="JNZ73_004491"
     CDS             484..879
                     /gene="frdC"
                     /locus_tag="JNZ73_004491"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY8734467.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            890..1249
                     /gene="frdD"
                     /locus_tag="JNZ73_004492"
     CDS             890..1249
                     /gene="frdD"
                     /locus_tag="JNZ73_004492"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY8734468.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            1312..2445
                     /gene="blaEC"
                     /locus_tag="JNZ73_004493"
     CDS             1312..2445
                     /gene="blaEC"
                     /locus_tag="JNZ73_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY8734469.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            2534..3067
                     /locus_tag="JNZ73_004494"
     CDS             2534..3067
                     /locus_tag="JNZ73_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY8734470.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3064..3258)
                     /locus_tag="JNZ73_004495"
                     /pseudo
     CDS             complement(3064..3258)
                     /locus_tag="JNZ73_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710017.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          787 a          850 c          849 g          772 t
ORIGIN      
        1 gtatgaaggt tgaagcgtta gctaacttcc cgattgaacg cgatctggtg gtcgatatga
       61 cccacttcat cgaaagtctg gaagcgatca aaccgtacat catcggcaac tcccgcaccg
      121 cggatcaggg tactaacatc cagaccccgg cgcagatggc aaagtatcac cagttctccg
      181 gttgcatcaa ctgtggtctg tgctacgccg cgtgcccgca gtttggcctg aacccagagt
      241 tcatcggtcc ggctgccatt acgctggcgc atcgttataa cgaagatagc cgcgaccacg
      301 gtaagaagga gcgtatggcg cagttgaaca gccagaacgg tgtatggagc tgtactttcg
      361 tgggctattg ctccgaagtc tgcccgaaac acgtcgatcc ggctgcggcc attcagcagg
      421 gcaaagtaga aagttcgaaa gactttctta tcgcgaccct gaaaccacgc taaggagtgc
      481 aacatgacga ctaaacgtaa accgtatgta cggccaatga cgtccacctg gtggaaaaaa
      541 ttgccgtttt atcgctttta catgctgcgc gaaggcacgg cggttccggc tgtgtggttc
      601 agcattgaac tgattttcgg gctgtttgcc ctgaaaaatg gcccggaagc ctgggcggga
      661 ttcgtcgact ttttacaaaa cccggttatc gtgatcatta acctgatcac tctgacggca
      721 gctctgctgc acaccaaaac ctggtttgaa ctggcaccga aagcggccaa tatcattgta
      781 aaagacgaaa aaatgggacc agagccaatt atcaaaagtc tctgggcggt aactgtggtt
      841 gccaccatcg taatcctgtt tgttgccctg tactggtaag gagcctgaga tgattaatcc
      901 aaatccaaag cgttctgacg aaccggtatt ctggggcctc ttcggggccg gtggtatgtg
      961 gagcgcaatt attgcgccgg tgatgatcct gctggtgggt attctgctgc cactggggct
     1021 gtttccgggt gatgcgctga gctacgagcg cgttctggcg ttcgcgcaga gcttcattgg
     1081 tcgcgtattc ctgttcctga tgatcgttct gccgctgtgg tgtggtttac accgtatgca
     1141 ccacgcgatg cacgatctga aaatccacgt gcctgcgggc aaatgggttt tctacggtct
     1201 ggctgctatc ctgacagttg tcacgctgat tggtgtcgtt acaatctaac gcatcgccaa
     1261 tgtaaatccg gcccgcctat ggcgggccgt tttgtatgga aaccagacct tatgttcaaa
     1321 acgacgctct gcgccttatt aattaccgcc tcttgctcca catttgctgc ccctcaacaa
     1381 atcaacgata ttgtgcatcg cacaattacc ccgcttatag agcaacaaaa gatccccggt
     1441 atggcggtgg cggtaattta tcagggtaaa ccttattact ttacctgggg ctatgcggac
     1501 atcgccaaaa agcagcccgt cacacagcaa acgttgtttg agttaggttc ggtcagcaaa
     1561 acatttacgg gcgtgcttgg tggcgacgct attgctcgag gggaaatcaa gttaagcgat
     1621 cccgcaacaa aatactggcc tgaacttacc gctaaacagt ggaatgggat cacactatta
     1681 catcttgcga cctacaccgc tggcggcctg ccattgcagg tgccggatga agtgaaatcc
     1741 tcaagcgact tgctgcgctt ctatcaaaac tggcagcctg catgggcccc aggaacacaa
     1801 cgtctgtatg ccaactccag tatcggtttg ttcggcgcac tggctgtgaa gccgtctggt
     1861 ttgagttttg agcaggcgat gcaaactcgt gtcttccagc cactcaaact caaccatacg
     1921 tggattaatg tacctccccc agaagaaaag aattacgcct ggggatatcg cgaaggtaag
     1981 gcagtgcatg tttcgccagg ggcgttagat gctgaagctt atggtgtgaa gtcgaccatt
     2041 gaagatatgg cccgctgggt gcaaagcaat ttaccccccc ttgatatcaa tgagaaaacg
     2101 cttcaacaag ggatacaact ggcacaatct cgctactggc aaaccggcga tatgtatcag
     2161 ggcctgggct gggaaatgct ggactggccg gtcaatcctg acagcatcat taacggcagt
     2221 gacaataaaa ttgcactggc agcacgcccg gtaaaagcga ttaagccccc aactcctgca
     2281 gtacgcgcat catgggtaca taaaacgggg gcgaccggcg gatttggtag ctatgtcgcg
     2341 tttattccag aaaaagagct gggtatcgtg atgctggcta acaaaaacta tcccaatcca
     2401 gagagagtcg ccgccgcctg tcagattctc aacgctctac agtaaaattt catcgggtcc
     2461 gaattttcgg accttttctg cgcttttcct tgctgtcatc tacacttaga aaaaaaccag
     2521 taaggaaaca tttatgcgcc tgctccctct cgttgccgca gcgacagctg catttctggt
     2581 cgttgcctgt agttctccta cgccgccgcg tggcgtgacc gtagtgaata atttcgacgc
     2641 caaacgttat ctcggtacct ggtatgagat tgcccgtttt gatcaccgtt ttgaacgtgg
     2701 actggaaaaa gtcaccgcaa catacagcct gcgtgatgac ggcggcctga atgtcattaa
     2761 taaaggctat aaccctgaca gaggaatgtg gcagcagagt gaagggaaag cgtactttac
     2821 cggcgcacca actcgcgctg cgctgaaagt gtcattcttt ggtcctttct atggcggtta
     2881 taacgttatt gcactcgatc gggaataccg ccatgcgctg gtttgcggcc cggaccgcga
     2941 ctacctgtgg atactctccc gcacgccaac catttctgac gaagtgaaac aggagatgct
     3001 ggcagtcgcg acccgggaag ggtttgatgt cagtaagttt atttgggtac agcagcccgg
     3061 tagttagtga gtgctgagtt tcagaccaat aatccccaat acgattaacg ccagactcgc
     3121 cagacgcatc gggttagcgg actcaccgag cagcacaatg ccggtgatgg ccgcgccgac
     3181 tgcgccaata cccgtccaca cggcatcaaa cggctaaagc cgtgggtata tttcaggcca
     3241 acggcccata ccacttcc
//
LOCUS       DADMGC010000428         3258 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.guided.239, whole genome shotgun sequence.
ACCESSION   DADMGC010000428 DADMGC010000000
VERSION     DADMGC010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.guided.239"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..473
                     /locus_tag="JNZ73_004496"
     CDS             <1..473
                     /locus_tag="JNZ73_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY8734471.1"
                     /translation="MKVEALANFPIERDLVVDMTHFIESLEAIKPYIIGNSRTADQGT
                     NIQTPAQMAKYHQFSGCINCGLCYAACPQFGLNPEFIGPAAITLAHRYNEDSRDHGKK
                     ERMAQLNSQNGVWSCTFVGYCSEVCPKHVDPAAAIQQGKVESSKDFLIATLKPR"
     gene            484..879
                     /gene="frdC"
                     /locus_tag="JNZ73_004497"
     CDS             484..879
                     /gene="frdC"
                     /locus_tag="JNZ73_004497"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY8734472.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            890..1249
                     /gene="frdD"
                     /locus_tag="JNZ73_004498"
     CDS             890..1249
                     /gene="frdD"
                     /locus_tag="JNZ73_004498"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY8734473.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            1312..2445
                     /gene="blaEC"
                     /locus_tag="JNZ73_004499"
     CDS             1312..2445
                     /gene="blaEC"
                     /locus_tag="JNZ73_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY8734474.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            2534..3067
                     /locus_tag="JNZ73_004500"
     CDS             2534..3067
                     /locus_tag="JNZ73_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY8734475.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3064..3258)
                     /locus_tag="JNZ73_004501"
                     /pseudo
     CDS             complement(3064..3258)
                     /locus_tag="JNZ73_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710017.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          787 a          849 c          849 g          773 t
ORIGIN      
        1 gtatgaaggt tgaagcgtta gctaacttcc cgattgaacg cgatctggtg gtcgatatga
       61 cccacttcat cgaaagtctg gaagcgatca aaccgtacat catcggcaac tcccgcaccg
      121 cggatcaggg tactaacatc cagaccccgg cgcagatggc aaagtatcac cagttctccg
      181 gttgcatcaa ctgtggtctg tgctacgccg cgtgcccgca gtttggcctg aacccagagt
      241 tcatcggtcc ggctgccatt acgctggcgc atcgttataa cgaagatagc cgcgaccacg
      301 gtaagaagga gcgtatggcg cagttgaaca gccagaacgg tgtatggagc tgtactttcg
      361 tgggctattg ctccgaagtc tgcccgaaac acgtcgatcc ggctgcggcc attcagcagg
      421 gcaaagtaga aagttcgaaa gactttctta tcgcgaccct gaaaccacgc taaggagtgc
      481 aacatgacga ctaaacgtaa accgtatgta cggccaatga cgtccacctg gtggaaaaaa
      541 ttgccgtttt atcgctttta catgctgcgc gaaggcacgg cggttccggc tgtgtggttc
      601 agcattgaac tgattttcgg gctgtttgcc ctgaaaaatg gcccggaagc ctgggcggga
      661 ttcgtcgact ttttacaaaa cccggttatc gtgatcatta acctgatcac tctgacggca
      721 gctctgctgc acaccaaaac ctggtttgaa ctggcaccga aagcggccaa tatcattgta
      781 aaagacgaaa aaatgggacc agagccaatt atcaaaagtc tctgggcggt aactgtggtt
      841 gccaccatcg taatcctgtt tgttgccctg tactggtaag gagcctgaga tgattaatcc
      901 aaatccaaag cgttctgacg aaccggtatt ctggggcctc ttcggggccg gtggtatgtg
      961 gagcgcaatt attgcgccgg tgatgatcct gctggtgggt attctgctgc cactggggct
     1021 gtttccgggt gatgcgctga gctacgagcg cgttctggcg ttcgcgcaga gcttcattgg
     1081 tcgcgtattc ctgttcctga tgatcgttct gccgctgtgg tgtggtttac accgtatgca
     1141 ccacgcgatg cacgatctga aaatccacgt gcctgcgggc aaatgggttt tctacggtct
     1201 ggctgctatc ctgacagttg tcacgctgat tggtgtcgtt acaatctaac gcatcgccaa
     1261 tgtaaatccg gcccgcctat ggcgggccgt tttgtatgga aaccagacct tatgttcaaa
     1321 acgacgctct gcgccttatt aattaccgcc tcttgctcca catttgctgc ccctcaacaa
     1381 atcaacgata ttgtgcatcg cacaattacc ccgcttatag agcaacaaaa gatccccggt
     1441 atggcggtgg cggtaattta tcagggtaaa ccttattact ttacttgggg ctatgcggac
     1501 atcgccaaaa agcagcccgt cacacagcaa acgttgtttg agttaggttc ggtcagcaaa
     1561 acatttacgg gcgtgcttgg tggcgacgct attgctcgag gggaaatcaa gttaagcgat
     1621 cccgcaacaa aatactggcc tgaacttacc gctaaacagt ggaatgggat cacactatta
     1681 catcttgcga cctacaccgc tggcggcctg ccattgcagg tgccggatga agtgaaatcc
     1741 tcaagcgact tgctgcgctt ctatcaaaac tggcagcctg catgggcccc aggaacacaa
     1801 cgtctgtatg ccaactccag tatcggtttg ttcggcgcac tggctgtgaa gccgtctggt
     1861 ttgagttttg agcaggcgat gcaaactcgt gtcttccagc cactcaaact caaccatacg
     1921 tggattaatg tacctccccc agaagaaaag aattacgcct ggggatatcg cgaaggtaag
     1981 gcagtgcatg tttcgccagg ggcgttagat gctgaagctt atggtgtgaa gtcgaccatt
     2041 gaagatatgg cccgctgggt gcaaagcaat ttaccccccc ttgatatcaa tgagaaaacg
     2101 cttcaacaag ggatacaact ggcacaatct cgctactggc aaaccggcga tatgtatcag
     2161 ggcctgggct gggaaatgct ggactggccg gtcaatcctg acagcatcat taacggcagt
     2221 gacaataaaa ttgcactggc agcacgcccg gtaaaagcga ttaagccccc aactcctgca
     2281 gtacgcgcat catgggtaca taaaacgggg gcgaccggcg gatttggtag ctatgtcgcg
     2341 tttattccag aaaaagagct gggtatcgtg atgctggcta acaaaaacta tcccaatcca
     2401 gagagagtcg ccgccgcctg tcagattctc aacgctctac agtaaaattt catcgggtcc
     2461 gaattttcgg accttttctg cgcttttcct tgctgtcatc tacacttaga aaaaaaccag
     2521 taaggaaaca tttatgcgcc tgctccctct cgttgccgca gcgacagctg catttctggt
     2581 cgttgcctgt agttctccta cgccgccgcg tggcgtgacc gtagtgaata atttcgacgc
     2641 caaacgttat ctcggtacct ggtatgagat tgcccgtttt gatcaccgtt ttgaacgtgg
     2701 actggaaaaa gtcaccgcaa catacagcct gcgtgatgac ggcggcctga atgtcattaa
     2761 taaaggctat aaccctgaca gaggaatgtg gcagcagagt gaagggaaag cgtactttac
     2821 cggcgcacca actcgcgctg cgctgaaagt gtcattcttt ggtcctttct atggcggtta
     2881 taacgttatt gcactcgatc gggaataccg ccatgcgctg gtttgcggcc cggaccgcga
     2941 ctacctgtgg atactctccc gcacgccaac catttctgac gaagtgaaac aggagatgct
     3001 ggcagtcgcg acccgggaag ggtttgatgt cagtaagttt atttgggtac agcagcccgg
     3061 tagttagtga gtgctgagtt tcagaccaat aatccccaat acgattaacg ccagactcgc
     3121 cagacgcatc gggttagcgg actcaccgag cagcacaatg ccggtgatgg ccgcgccgac
     3181 tgcgccaata cccgtccaca cggcatcaaa cggctaaagc cgtgggtata tttcaggcca
     3241 acggcccata ccacttcc
//
LOCUS       DADMGC010000429         1803 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.guided.293, whole genome shotgun sequence.
ACCESSION   DADMGC010000429 DADMGC010000000
VERSION     DADMGC010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.guided.293"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            complement(<1..428)
                     /locus_tag="JNZ73_004502"
     CDS             complement(<1..428)
                     /locus_tag="JNZ73_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HAY8734476.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLT"
     gene            690..1148
                     /gene="gpt"
                     /locus_tag="JNZ73_004503"
     CDS             690..1148
                     /gene="gpt"
                     /locus_tag="JNZ73_004503"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY8734477.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1240..>1803
                     /locus_tag="JNZ73_004504"
     CDS             1240..>1803
                     /locus_tag="JNZ73_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY8734478.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMP"
BASE COUNT          439 a          462 c          464 g          438 t
ORIGIN      
        1 atggtcagca gcacttccag cgggccgtga accacgtttt cgtcagccag aaccgccagt
       61 gcagaggcca taccaatgcc gttatccgca cccagcgtgg tgccgcgcgc tttaacccat
      121 tcgccatcaa tataaggctg gataggatct ttcgtgaagt catgcacggt gtcgttattt
      181 ttctgcggca ccatatcgag gtgggcctgt aagacgaccg gtttacgatt ttccatacct
      241 gcggtagcag gtttacgaat caggatatta cctacctgat cgcgttcgac atggaaacct
      301 ttctcttttg cccaaccaac aatgtattca gcgagttgct cttcatgata ggacgggtga
      361 ggaatagaac agattttggc aaaaatatcc cacagcggct gtggagataa ttgagacagt
      421 tcagacacgt taagtctcct tgtcgatcac ccgcaaaaca gtattgcagg tcacagggtt
      481 agcagaaaat gttgtcaaca caagacaggc ttgcgagata tgtattgaga ataccacttt
      541 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
      601 ttagtgcgcc agatatctat aatctcgcgc aacctatttt cccctcgaac actttttaag
      661 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
      721 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
      781 gcattattgc cgtaagccgt ggcggtctgg taccgggggc gttactggcg cgtgaactgg
      841 gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc
      901 ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg
      961 tggataccgg tggtactgct gttgcgattc gtgaaatgta tccaaaagcg cactttgtca
     1021 ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc
     1081 aagatacctg gatcgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg
     1141 gtcgctaatc ttttcaacgc ctggcattgc cgggcgttgt tctttttaac ttcaggcagg
     1201 ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc
     1261 gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt
     1321 aatcacggcg cacaaccgcc tgtgcagtcg gctcttgatg gtaaaaccat ccctcactgg
     1381 tatcgcatga ttaaccgtct gatgtggatc tggcgcggta ttgacccacg tgaaatcctc
     1441 gacgtccagg cacgtattgt gatgagcgat gctgaacgta ccgacgatga tttatacgat
     1501 accgtgattg gctaccgtgg tggcaactgg atttatgagt gggccaccca ggcgatggtg
     1561 tggcaacaaa aagcctgtac ggaagaagat ccgcaactca gtggtcgaca ctggctgcat
     1621 gcggctacgt tgtacaacat tgccgcctat cctcatctga aaggagatga cctggccgag
     1681 caagcgcagg ctttatcaaa ccgcgcctat gaagaggccg cccagcgtct gccgggcacg
     1741 atgcggcaga tggagtttac cgtacccggc ggtgcgccca tcaccggctt tttgcatatg
     1801 ccg
//
LOCUS       DADMGC010000430         1740 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.guided.298, whole genome shotgun sequence.
ACCESSION   DADMGC010000430 DADMGC010000000
VERSION     DADMGC010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.guided.298"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            264..923
                     /locus_tag="JNZ73_004505"
     CDS             264..923
                     /locus_tag="JNZ73_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HAY8734479.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1124..1501)
                     /locus_tag="JNZ73_004506"
     CDS             complement(1124..1501)
                     /locus_tag="JNZ73_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8734480.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1568..>1740)
                     /locus_tag="JNZ73_004507"
     CDS             complement(1568..>1740)
                     /locus_tag="JNZ73_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8734481.1"
                     /translation="AAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSAKIGA
                     GKFRPLRPLQPA"
BASE COUNT          457 a          406 c          421 g          456 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     1201 ccagagcctg ataaaaacgg ttagcgccgg ggttggattt ttcagcgttc cagctaaggc
     1261 taaggcattc ctgttcaagc gcaagccggg ctataaagcg cattatcgct ttacccgtgc
     1321 ccttatttcg atcgcactga gaaacataca gctctttaat atgcagctga ccgctgtatc
     1381 ggggcgaggg ataaagaata ttgcaacatg ccaggccagt aatgttattg ccgcagcgcg
     1441 ctctgatcac cagggtaccg gaaagccggt tgaataactt tttacagaga taatccttca
     1501 tcaacgcttc ctgaatgata ccttcgccat aataggggtc gtctcagaaa acggaaaata
     1561 aagcacgcta agccggttgc agaggccgta gcggcctgaa cttccccgcg ccgatcttgg
     1621 cgctgctgcg ccataggtaa tcaccggtca ggttgatgtg ctcccagccg agtggcgaca
     1681 ggtactgcaa tagcgagtca tcgacggcat gaccattgcc gcgcaacgca tgcgccgcac
//
LOCUS       DADMGC010000431         1536 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.guided.306, whole genome shotgun sequence.
ACCESSION   DADMGC010000431 DADMGC010000000
VERSION     DADMGC010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.guided.306"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            59..532
                     /locus_tag="JNZ73_004508"
     CDS             59..532
                     /locus_tag="JNZ73_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HAY8734482.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVVIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            625..1416
                     /gene="aadA1"
                     /locus_tag="JNZ73_004509"
     CDS             625..1416
                     /gene="aadA1"
                     /locus_tag="JNZ73_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY8734483.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          420 a          355 c          389 g          372 t
ORIGIN      
        1 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aacctctgag gaagaattgt
       61 gaaactatca ctaatggtag ctatatcgaa gaatggagtt atcgggaatg gccctgatat
      121 tccatggagt gccaaaggtg aacagctcct gtttaaagct attacctata accaatggct
      181 gttggttgga cgcaagactt ttgaatcaat gggagcatta cccaaccgaa agtatgcggt
      241 cgtaacacgt tcaagtttta catctgacaa tgagaacgta gtgatctttc catcaattaa
      301 agatgcttta accaacctaa agaaaataac ggatcatgtc attgtttcag gtggtgggga
      361 gatatacaaa agcctgatcg atcaagtaga tacactacat atatctacaa tagacatcga
      421 gccggaaggt gatgtttact ttcctgaaat ccccagcaat tttaggccag tttttaccca
      481 agacttcgcc tctaacataa attatagtta ccaaatctgg caaaagggtt aacaagtggc
      541 agcaacggat tcgcaaacct gtcacgcctt ttgtaccaaa agccgcgcca ggtttgcgat
      601 ccgctgtgcc aggcgttaaa catcatgagg gaagcggtga tcgccgaagt atcgactcaa
      661 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
      721 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt
      781 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
      841 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
      901 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
      961 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
     1021 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     1081 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     1141 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     1201 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     1261 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
     1321 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgttcactac
     1381 gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc taacaattcg ttcaagccga
     1441 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgcggtg
     1501 atgctgccaa cttactgatt tagtgtatga tggtgt
//
LOCUS       DADMGC010000432         1283 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.guided.321, whole genome shotgun sequence.
ACCESSION   DADMGC010000432 DADMGC010000000
VERSION     DADMGC010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.guided.321"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            289..1104
                     /gene="sul2"
                     /locus_tag="JNZ73_004510"
     CDS             289..1104
                     /gene="sul2"
                     /locus_tag="JNZ73_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAY8734484.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1165..>1283
                     /locus_tag="JNZ73_004511"
     CDS             1165..>1283
                     /locus_tag="JNZ73_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3'') family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAY8734485.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIA"
BASE COUNT          273 a          352 c          339 g          319 t
ORIGIN      
        1 tttgtagatt actatcaatt aatcttccga acatgacacc agtcactgtt gattacggtt
       61 aaaccgctcc gtaacaggtt tccctcgtat tggtgacttg cagcaccaaa gaagcaaaaa
      121 agataaatat atctacttat tgagagatga tatatttatt tccaaaatgc tacagataca
      181 tcatcatgaa acggatcacc gcaaacaggt tactcgcctg gggattccct ttcgacccga
      241 gcatccgtat gagactcatg ctcgattatt attattatag aagcccccat gaataaatcg
      301 ctcatcattt tcggcatcgt caacataacc tcggacagtt tctccgatgg aggccggtat
      361 ctggcgccag acgcagccat tgcgcaggcg cgtaagctga tggccgaggg ggcagatgtg
      421 atcgacctcg gtccggcatc cagcaatccc gacgccgcgc ctgtttcgtc cgacacagaa
      481 atcgcgcgta tcgcgccggt gctggacgcg ctcaaggcag atggcattcc cgtctcgctc
      541 gacagttatc aacccgcgac gcaagcctat gccttgtcgc gtggtgtggc ctatctcaat
      601 gatattcgcg gttttccaga cgctgcgttc tatccgcaat tggcgaaatc atctgccaaa
      661 ctcgtcgtta tgcattcggt gcaagacggg caggcagatc ggcgcgaggc acccgctggc
      721 gacatcatgg atcacattgc ggcgttcttt gacgcgcgca tcgcggcgct gacgggtgcc
      781 ggtatcaaac gcaaccgcct tgtccttgat cccggcatgg ggttttttct gggggctgct
      841 cccgaaacct cgctctcggt gctggcgcgg ttcgatgaat tgcggctgcg cttcgatttg
      901 ccggtgcttc tgtctgtttc gcgcaaatcc tttctgcgcg cgctcacagg ccgtggtccg
      961 ggggatgtcg gggccgcgac actcgctgca gagcttgccg ccgccgcagg tggagctgac
     1021 ttcatccgca cacacgagcc gcgccccttg cgcgacgggc tggcggtatt ggcggcgctg
     1081 aaagaaaccg caagaattcg ttaactgcac attcgggata tttctctata ttcgcgcttc
     1141 atcagaaaac tgaaggaacc tccattgaat cgaactaata ttttttttgg tgaatcgcat
     1201 tctgactggt tgcctgtcag aggcggagaa tctggtgatt ttgtttttcg acgtggtgac
     1261 gggcatgcct tcgcgaaaat cgc
//
LOCUS       DADMGC010000433          990 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.guided.341, whole genome shotgun sequence.
ACCESSION   DADMGC010000433 DADMGC010000000
VERSION     DADMGC010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.guided.341"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..463
                     /locus_tag="JNZ73_004512"
     CDS             <1..463
                     /locus_tag="JNZ73_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8734486.1"
                     /translation="IRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNASELRGL
                     HGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIAQMADA
                     FSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
     gene            complement(435..974)
                     /locus_tag="JNZ73_004513"
     CDS             complement(435..974)
                     /locus_tag="JNZ73_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY8734487.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFGVVCGKGRNPTLRLWPAIFSGEIDV
                     FPGDRRSRLISSMTSVAPA"
BASE COUNT          220 a          297 c          272 g          201 t
ORIGIN      
        1 gatccgggat cgctttgcag ctttgtttca gcgggcgcgc gatgatcaaa acgcaggttg
       61 tcaaactgac tacgtccacg cggcgattat agccgatcaa atgatgagca atgcctcgga
      121 actgcgtggg ctacatggcg atctgcatca tgaaaacatc atgttctcca gtcgcggctg
      181 gctggtgata gatcccgtcg gtctggtcgg tgaagtgggc tttggcgccg ccaatatgtt
      241 ctacgatccg gctgacagag acgacctttg tctcgatcct agacgcattg cacagatggc
      301 ggacgcattc tctcgtgcgc tggacgtcga tccgcgtcgc ctgctcgacc aggcgtacgc
      361 ttatgggtgc ctttccgcag cttggaacgc ggatggagaa gaggagcaac gcgatctagc
      421 tatcgcggcc gcgatcaagc aggtgcgaca gacgtcatac tagatatcaa gcgacttctc
      481 ctatcccctg ggaacacatc aatctcaccg gagaatatcg ctggccaaag ccttagcgta
      541 ggattccgcc ccttcccgca aacgacccca aacaggaaac gcagctgaaa cgggaagctc
      601 aacacccact gacgcatggg ttgttcaggc agtacttcat caaccagcaa ggcggcactt
      661 tcggccatcc gccgcgcccc acagctcggg cagaaaccgc gacgcttaca gctgaaagcg
      721 accaggtgct cggcgtggca agactcgcag cgaacccgta gaaagccatg ctccagccgc
      781 ccgcattgga gaaattcttc aaattcccgt tgcacatagc ccggcaattc ctttccctgc
      841 tctgccataa gcgcagcgaa tgccgggtaa tactcgtcaa cgatctgata gagaagggtt
      901 tgctcgggtc ggtggctctg gtaacgacca gtatcccgat cccggctggc cgtcctggcc
      961 gccacatgag gcatgttccg cgtccttgca
//
LOCUS       DADMGC010000434          801 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180069
            SAMEA1710238-rid11411003.guided.367, whole genome shotgun sequence.
ACCESSION   DADMGC010000434 DADMGC010000000
VERSION     DADMGC010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710238
            Sequence Read Archive: ERR230499
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941159.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 04:11:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 193 of 375
            Pseudo Genes (incomplete)         :: 82 of 375
            Pseudo Genes (internal stop)      :: 135 of 375
            Pseudo Genes (multiple problems)  :: 33 of 375
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710238-rid11411003.guided.367"
                     /strain="ERS180069"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2011"
     gene            <1..638
                     /locus_tag="JNZ73_004514"
     CDS             <1..638
                     /locus_tag="JNZ73_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HAY8734488.1"
                     /translation="QMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGMEIWNSNH
                     TPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLTEAWLESS
                     LKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRT
                     LQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL"
BASE COUNT          305 a          155 c          148 g          193 t
ORIGIN      
        1 cgcaaatggc accagattca actttcaaga tcgcattatc acttatggca tttgatgcgg
       61 aaataataga tcagaaaacc atattcaaat gggataaaac ccccaaagga atggagatct
      121 ggaacagcaa tcatacacca aagacgtgga tgcaattttc tgttgtttgg gtttcgcaag
      181 aaataaccca aaaaattgga ttaaataaaa tcaagaatta tctcaaagat tttgattatg
      241 gaaatcaaga cttctctgga gataaagaaa gaaacaacgg attaacagaa gcatggctcg
      301 aaagtagctt aaaaatttca ccagaagaac aaattcaatt cctgcgtaaa attattaatc
      361 acaatctccc agttaaaaac tcagccatag aaaacaccat agagaacatg tatctacaag
      421 atctggataa tagtacaaaa ctgtatggga aaactggtgc aggattcaca gcaaatagaa
      481 ccttacaaaa cggatggttt gaagggttta ttataagcaa atcaggacat aaatatgttt
      541 ttgtgtccgc acttacagga aacttggggt cgaatttaac atcaagcata aaagccaaga
      601 aaaatgcgat caccattcta aacacactaa atttataaaa aatctaatgg caaaatcgcc
      661 caacccttca atcaagtcgg gacggccaaa agcaagcttt tggctcccct cgctggcgct
      721 cggcgcccct tatttcaaac gttaaacatc atgagggaag tggtgatcgc cgaagtatcg
      781 actcaactat cagaggtagt t
//
